LOCUS       NP_001191949             285 aa            linear   INV 19-NOV-2025
DEFINITION  vacuolar protein sorting-associated protein VTA1 homolog
            [Acyrthosiphon pisum].
ACCESSION   NP_001191949 XP_001948982
VERSION     NP_001191949.1
DBSOURCE    REFSEQ: accession NM_001205020.1
KEYWORDS    RefSeq.
SOURCE      Acyrthosiphon pisum (pea aphid)
  ORGANISM  Acyrthosiphon pisum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Paraneoptera; Hemiptera; Sternorrhyncha;
            Aphidomorpha; Aphidoidea; Aphididae; Macrosiphini; Acyrthosiphon.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            EX631892.1, EX609385.1, HP245475.1 and EX613206.1.
            
            On Mar 19, 2011 this sequence version replaced XP_001948982.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK340185.1 [ECO:0000332]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Acyrthosiphon pisum"
                     /db_xref="taxon:7029"
                     /chromosome="A1"
                     /map="A1"
     Protein         1..285
                     /product="vacuolar protein sorting-associated protein VTA1
                     homolog"
                     /note="Vps20-associated 1 homolog"
                     /calculated_mol_wt=31788
     Region          19..149
                     /region_name="Vta1"
                     /note="Vta1 like; pfam04652"
                     /db_xref="CDD:461380"
     Region          243..280
                     /region_name="Vta1_C"
                     /note="Vta1 C-terminal domain; pfam18097"
                     /db_xref="CDD:465647"
     CDS             1..285
                     /gene="Vta1"
                     /coded_by="NM_001205020.1:446..1303"
                     /db_xref="APHIDBASE:ACYPI009424"
                     /db_xref="GeneID:100168747"
ORIGIN      
        1 maqipecpes lkkiqhhlki alehdskdpv isywcrlyal qaaltldkss kdakmflvsl
       61 mdwlekqknn lkdndmitne taaqahieny aiklfnfadg mdrqanynkn ivklfftagl
      121 lmdvlsvfgd vseeitntqk yakwkatyih ncmkngetpt pgppntesgq igfnfpnqge
      181 diqqptdnca pltptkhtye dypasnpney qqisnmstis ptevqptvsg rpillrdgiq
      241 lspsqitkaq kyckfaasal tyddvsesia nlqkalkllt tgeds
//
LOCUS       NP_001136224             119 aa            linear   INV 19-NOV-2025
DEFINITION  protein anoxia up-regulated protein fau [Bombyx mori].
ACCESSION   NP_001136224
VERSION     NP_001136224.1
DBSOURCE    REFSEQ: accession NM_001142752.2
KEYWORDS    RefSeq.
SOURCE      Bombyx mori (domestic silkworm)
  ORGANISM  Bombyx mori
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Lepidoptera; Glossata;
            Ditrysia; Bombycoidea; Bombycidae; Bombycinae; Bombyx.
REFERENCE   1  (residues 1 to 119)
  AUTHORS   Ou,J., Deng,H.M., Zheng,S.C., Huang,L.H., Feng,Q.L. and Liu,L.
  TITLE     Transcriptomic analysis of developmental features of Bombyx mori
            wing disc during metamorphosis
  JOURNAL   BMC Genomics 15 (1), 820 (2014)
   PUBMED   25261999
  REMARK    Publication Status: Online-Only
REFERENCE   2  (residues 1 to 119)
  AUTHORS   Suetsugu,Y., Futahashi,R., Kanamori,H., Kadono-Okuda,K.,
            Sasanuma,S., Narukawa,J., Ajimura,M., Jouraku,A., Namiki,N.,
            Shimomura,M., Sezutsu,H., Osanai-Futahashi,M., Suzuki,M.G.,
            Daimon,T., Shinoda,T., Taniai,K., Asaoka,K., Niwa,R., Kawaoka,S.,
            Katsuma,S., Tamura,T., Noda,H., Kasahara,M., Sugano,S., Suzuki,Y.,
            Fujiwara,H., Kataoka,H., Arunkumar,K.P., Tomar,A., Nagaraju,J.,
            Goldsmith,M.R., Feng,Q., Xia,Q., Yamamoto,K., Shimada,T. and
            Mita,K.
  TITLE     Large scale full-length cDNA sequencing reveals a unique genomic
            landscape in a lepidopteran model insect, Bombyx mori
  JOURNAL   G3 (Bethesda) 3 (9), 1481-1492 (2013)
   PUBMED   23821615
  REMARK    Publication Status: Online-Only
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from FJ425872.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FJ425872.1, FY740682.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00506772, SAMN35672757
                                           [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..119
                     /organism="Bombyx mori"
                     /db_xref="taxon:7091"
                     /chromosome="25"
                     /map="25"
     Protein         1..119
                     /product="protein anoxia up-regulated protein fau"
                     /calculated_mol_wt=13284
     CDS             1..119
                     /gene="fau"
                     /coded_by="NM_001142752.2:38..397"
                     /db_xref="GeneID:100216499"
ORIGIN      
        1 mvyesdfytt rrpyrstysv ttprhyvvvd rdpiaprrae eqysysysst sqrsagsglp
       61 qrssysntve rrtgsgpggy sytserssnl ggpggysyss tssgrlpygt tyrhysyrv
//
LOCUS       XP_077501005             149 aa            linear   INV 25-NOV-2025
DEFINITION  N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D-like
            [Amblyomma americanum].
ACCESSION   XP_077501005
VERSION     XP_077501005.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644879.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..149
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..149
                     /product="N-acyl-phosphatidylethanolamine-hydrolyzing
                     phospholipase D-like"
                     /calculated_mol_wt=17038
     Region          <16..120
                     /region_name="UlaG"
                     /note="L-ascorbate lactonase UlaG, metallo-beta-lactamase
                     superfamily [Carbohydrate transport and metabolism];
                     COG2220"
                     /db_xref="CDD:441822"
     Region          <16..66
                     /region_name="metallo-hydrolase-like_MBL-fold"
                     /note="mainly hydrolytic enzymes and related proteins
                     which carry out various biological functions; MBL-fold
                     metallohydrolase domain; cl23716"
                     /db_xref="CDD:451500"
     CDS             1..149
                     /gene="LOC144111860"
                     /coded_by="XM_077644879.1:122..571"
                     /db_xref="GeneID:144111860"
ORIGIN      
        1 mltdylgvil fllqvlwggw cvispeyrfy fagdtgycdv fkqigqaygp fdvaaipiga
       61 yeprwfmkyq hvnpeeavli hkdvrsratl avhwgtftla neyyldppvk lresldrhgi
      121 sprefftlkh gesrllhatt snrtsrvlp
//
LOCUS       XP_077501006             696 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 1-like isoform
            X1 [Amblyomma americanum].
ACCESSION   XP_077501006
VERSION     XP_077501006.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644880.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..696
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 1-like isoform X1"
                     /calculated_mol_wt=79763
     Region          <23..82
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          23..50
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(24..25,28..30,32..33,37,40,50,52,54,58..59,62..64,
                     66..67,71,74,83,85,87,91,94,96..97,101,104)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          52..83
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          113..229
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          278..393
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          392..>447
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     Region          <464..617
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..696
                     /gene="LOC144111861"
                     /coded_by="XM_077644880.1:116..2206"
                     /db_xref="GeneID:144111861"
ORIGIN      
        1 mrlekdalia eshdglrrnt lelflscrtg dlarvkylve eqeselnvrd rwdgtplyya
       61 clcghkdvve yllfqgascv antfdgercl yaslsleird llrdrkvits ntmrrdtyde
      121 flrrcledsq hcdvsfsvqg eiipahrcvl aarcdffrrs lldkwagrql ipvthhavdg
      181 avfriitqyl ytgrlemqak hfesflelas rcqlisliad vkealaevln qgsqrsgvsa
      241 esetillept hyreqlqrdf stlpvelasg vtaggaafpa eehnhadici nvdgthflch
      301 kvflcnrsey fraliedhft easrlgsdqq qlpvielqqv tpnifgcvlr hvysnsddrv
      361 natnvwdvlc aadvyllpdl krqcgaqiar lldvesicgt lrasrlfrlp rlenqciefm
      421 akhlaeviel pefheaired akevklrsfl kthfyyyfyp fyhavcyftg citffavasf
      481 krskmpkvlq atawciavac gmccifmktv wyrtrdptte fgklsaaffd rilwsiflaw
      541 itlscatgrg gflnkflsws aftplsrlaf gvyiihlpfi hlclhisrer iffshfsvvs
      601 fffsmlvwsy llsyllfifc eaptgrldkl ifdpqrrkte grekdeapnd nsvqdklpda
      661 vvtfrdrkld iwqtsseknt sngilpgncs rtschl
//
LOCUS       XP_077501007             638 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 1-like isoform
            X2 [Amblyomma americanum].
ACCESSION   XP_077501007
VERSION     XP_077501007.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644881.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..638
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 1-like isoform X2"
                     /calculated_mol_wt=73024
     Region          <23..>106
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          23..50
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(24..25,28..30,32..33,37,40,50,52,54,58..59,62..64,
                     66..67,71,74,83,85,87,91,94,96..97,101,104)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          52..83
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          113..229
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          278..335
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          334..>389
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     Region          <406..559
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..638
                     /gene="LOC144111861"
                     /coded_by="XM_077644881.1:116..2032"
                     /db_xref="GeneID:144111861"
ORIGIN      
        1 mrlekdalia eshdglrrnt lelflscrtg dlarvkylve eqeselnvrd rwdgtplyya
       61 clcghkdvve yllfqgascv antfdgercl yaslsleird llrdrkvits ntmrrdtyde
      121 flrrcledsq hcdvsfsvqg eiipahrcvl aarcdffrrs lldkwagrql ipvthhavdg
      181 avfriitqyl ytgrlemqak hfesflelas rcqlisliad vkealaevln qgsqrsgvsa
      241 esetillept hyreqlqrdf stlpvelasg vtaggaafpa eehnhadici nvdgthflch
      301 kvnatnvwdv lcaadvyllp dlkrqcgaqi arlldvesic gtlrasrlfr lprlenqcie
      361 fmakhlaevi elpefheair edakevklrs flkthfyyyf ypfyhavcyf tgcitffava
      421 sfkrskmpkv lqatawciav acgmccifmk tvwyrtrdpt tefgklsaaf fdrilwsifl
      481 awitlscatg rggflnkfls wsaftplsrl afgvyiihlp fihlclhisr eriffshfsv
      541 vsfffsmlvw syllsyllfi fceaptgrld klifdpqrrk tegrekdeap ndnsvqdklp
      601 davvtfrdrk ldiwqtssek ntsngilpgn csrtschl
//
LOCUS       XP_077501008             584 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 1-like isoform
            X3 [Amblyomma americanum].
ACCESSION   XP_077501008
VERSION     XP_077501008.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644882.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..584
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 1-like isoform X3"
                     /calculated_mol_wt=66904
     Region          1..117
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          166..281
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          280..>335
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     Region          <352..505
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..584
                     /gene="LOC144111861"
                     /coded_by="XM_077644882.1:331..2085"
                     /db_xref="GeneID:144111861"
ORIGIN      
        1 mrrdtydefl rrcledsqhc dvsfsvqgei ipahrcvlaa rcdffrrsll dkwagrqlip
       61 vthhavdgav friitqylyt grlemqakhf esflelasrc qlisliadvk ealaevlnqg
      121 sqrsgvsaes etillepthy reqlqrdfst lpvelasgvt aggaafpaee hnhadicinv
      181 dgthflchkv flcnrseyfr aliedhftea srlgsdqqql pvielqqvtp nifgcvlrhv
      241 ysnsddrvna tnvwdvlcaa dvyllpdlkr qcgaqiarll dvesicgtlr asrlfrlprl
      301 enqciefmak hlaevielpe fheairedak evklrsflkt hfyyyfypfy havcyftgci
      361 tffavasfkr skmpkvlqat awciavacgm ccifmktvwy rtrdpttefg klsaaffdri
      421 lwsiflawit lscatgrggf lnkflswsaf tplsrlafgv yiihlpfihl clhisrerif
      481 fshfsvvsff fsmlvwsyll syllfifcea ptgrldklif dpqrrktegr ekdeapndns
      541 vqdklpdavv tfrdrkldiw qtssekntsn gilpgncsrt schl
//
LOCUS       XP_077501009             149 aa            linear   INV 25-NOV-2025
DEFINITION  N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D-like
            [Amblyomma americanum].
ACCESSION   XP_077501009
VERSION     XP_077501009.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644883.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..149
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..149
                     /product="N-acyl-phosphatidylethanolamine-hydrolyzing
                     phospholipase D-like"
                     /calculated_mol_wt=17038
     Region          <16..120
                     /region_name="UlaG"
                     /note="L-ascorbate lactonase UlaG, metallo-beta-lactamase
                     superfamily [Carbohydrate transport and metabolism];
                     COG2220"
                     /db_xref="CDD:441822"
     Region          <16..66
                     /region_name="metallo-hydrolase-like_MBL-fold"
                     /note="mainly hydrolytic enzymes and related proteins
                     which carry out various biological functions; MBL-fold
                     metallohydrolase domain; cl23716"
                     /db_xref="CDD:451500"
     CDS             1..149
                     /gene="LOC144111862"
                     /coded_by="XM_077644883.1:122..571"
                     /db_xref="GeneID:144111862"
ORIGIN      
        1 mltdylgvil fllqvlwggw cvispeyrfy fagdtgycdv fkqigqaygp fdvaaipiga
       61 yeprwfmkyq hvnpeeavli hkdvrsratl avhwgtftla neyyldppvk lresldrhgi
      121 sprefftlkh gesrllhatt snrtsrvlp
//
LOCUS       XP_077501011             696 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 1-like isoform
            X1 [Amblyomma americanum].
ACCESSION   XP_077501011
VERSION     XP_077501011.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644885.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..696
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 1-like isoform X1"
                     /calculated_mol_wt=79616
     Region          <23..>106
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          23..50
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(24..25,28..30,32..33,37,40,50,52,54,58..59,62..64,
                     66..67,71,74,83,85,87,91,94,96..97,101,104)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          52..83
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          113..229
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          278..393
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          392..>447
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     Region          <463..617
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..696
                     /gene="LOC144111863"
                     /coded_by="XM_077644885.1:76..2166"
                     /db_xref="GeneID:144111863"
ORIGIN      
        1 mrlekdalia eshdglrrnt lelflscrtg dlarvkylve eqeselnvrd rwdgtplyya
       61 clcghkdvve yllsqgascv antfdgercl yaslsleird llrdrkvits ntmrrdtyde
      121 flrrcledsq hcdvsfsvqg eiipahrcvl aarcdffrss lldkwagrql ipvthhavdg
      181 avfriitqyl ytgrlemqak hfesflelas rcqlisliad vkealaevln qgsqrsgvsa
      241 esetillept hyreqlqrdf stlpvelasg vtaggaafpa eehnhadici nvdgthflch
      301 kvflcnrsey fraliedhft easrlgsdqq qlpvielqqv tpnifgcvlr hvysnsddrv
      361 natnvwdvlc aadvyllpdl krqcgaqiar lldvesicgt lrasrlfrlp rlenqciefm
      421 akhlaeviel pefheaired akevklrsfl kthfyyyfyp fyhavcyftg citffavasf
      481 krskmpkvlq atawciavac gmccifiktv wyrtrdptte fgklsaaffd rilwsiflaw
      541 itlscatgrg gflnkflsws aftplsrlaf gvyiihlpfi hlclhisrer iffshfsvvs
      601 fffsmlvwsy llsyllfifc eaptgrldkl ifdpqrrkte grekdeapnd nsvqdklpda
      661 vvtfrdrkld iwqtsseknt sngilpgncs rtschl
//
LOCUS       XP_077501012             658 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 1-like isoform
            X2 [Amblyomma americanum].
ACCESSION   XP_077501012
VERSION     XP_077501012.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644886.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..658
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 1-like isoform X2"
                     /calculated_mol_wt=75054
     Region          75..191
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          240..355
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          354..>409
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     Region          <425..579
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..658
                     /gene="LOC144111863"
                     /coded_by="XM_077644886.1:176..2152"
                     /db_xref="GeneID:144111863"
ORIGIN      
        1 mgrhtsvsgs isrpdkllry yaclcghkdv veyllsqgas cvantfdger clyaslslei
       61 rdllrdrkvi tsntmrrdty deflrrcled sqhcdvsfsv qgeiipahrc vlaarcdffr
      121 sslldkwagr qlipvthhav dgavfriitq ylytgrlemq akhfesflel asrcqlisli
      181 advkealaev lnqgsqrsgv saesetille pthyreqlqr dfstlpvela sgvtaggaaf
      241 paeehnhadi cinvdgthfl chkvflcnrs eyfraliedh fteasrlgsd qqqlpvielq
      301 qvtpnifgcv lrhvysnsdd rvnatnvwdv lcaadvyllp dlkrqcgaqi arlldvesic
      361 gtlrasrlfr lprlenqcie fmakhlaevi elpefheair edakevklrs flkthfyyyf
      421 ypfyhavcyf tgcitffava sfkrskmpkv lqatawciav acgmccifik tvwyrtrdpt
      481 tefgklsaaf fdrilwsifl awitlscatg rggflnkfls wsaftplsrl afgvyiihlp
      541 fihlclhisr eriffshfsv vsfffsmlvw syllsyllfi fceaptgrld klifdpqrrk
      601 tegrekdeap ndnsvqdklp davvtfrdrk ldiwqtssek ntsngilpgn csrtschl
//
LOCUS       XP_077501013             638 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 1-like isoform
            X3 [Amblyomma americanum].
ACCESSION   XP_077501013
VERSION     XP_077501013.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644887.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..638
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 1-like isoform X3"
                     /calculated_mol_wt=72877
     Region          <23..>106
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          23..50
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(24..25,28..30,32..33,37,40,50,52,54,58..59,62..64,
                     66..67,71,74,83,85,87,91,94,96..97,101,104)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          52..83
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          113..229
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          278..335
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          334..>389
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     Region          <405..559
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..638
                     /gene="LOC144111863"
                     /coded_by="XM_077644887.1:76..1992"
                     /db_xref="GeneID:144111863"
ORIGIN      
        1 mrlekdalia eshdglrrnt lelflscrtg dlarvkylve eqeselnvrd rwdgtplyya
       61 clcghkdvve yllsqgascv antfdgercl yaslsleird llrdrkvits ntmrrdtyde
      121 flrrcledsq hcdvsfsvqg eiipahrcvl aarcdffrss lldkwagrql ipvthhavdg
      181 avfriitqyl ytgrlemqak hfesflelas rcqlisliad vkealaevln qgsqrsgvsa
      241 esetillept hyreqlqrdf stlpvelasg vtaggaafpa eehnhadici nvdgthflch
      301 kvnatnvwdv lcaadvyllp dlkrqcgaqi arlldvesic gtlrasrlfr lprlenqcie
      361 fmakhlaevi elpefheair edakevklrs flkthfyyyf ypfyhavcyf tgcitffava
      421 sfkrskmpkv lqatawciav acgmccifik tvwyrtrdpt tefgklsaaf fdrilwsifl
      481 awitlscatg rggflnkfls wsaftplsrl afgvyiihlp fihlclhisr eriffshfsv
      541 vsfffsmlvw syllsyllfi fceaptgrld klifdpqrrk tegrekdeap ndnsvqdklp
      601 davvtfrdrk ldiwqtssek ntsngilpgn csrtschl
//
LOCUS       XP_077501014             584 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 1-like isoform
            X4 [Amblyomma americanum].
ACCESSION   XP_077501014
VERSION     XP_077501014.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644888.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..584
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 1-like isoform X4"
                     /calculated_mol_wt=66817
     Region          1..117
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          166..281
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          280..>335
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     Region          <374..505
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..584
                     /gene="LOC144111863"
                     /coded_by="XM_077644888.1:248..2002"
                     /db_xref="GeneID:144111863"
ORIGIN      
        1 mrrdtydefl rrcledsqhc dvsfsvqgei ipahrcvlaa rcdffrssll dkwagrqlip
       61 vthhavdgav friitqylyt grlemqakhf esflelasrc qlisliadvk ealaevlnqg
      121 sqrsgvsaes etillepthy reqlqrdfst lpvelasgvt aggaafpaee hnhadicinv
      181 dgthflchkv flcnrseyfr aliedhftea srlgsdqqql pvielqqvtp nifgcvlrhv
      241 ysnsddrvna tnvwdvlcaa dvyllpdlkr qcgaqiarll dvesicgtlr asrlfrlprl
      301 enqciefmak hlaevielpe fheairedak evklrsflkt hfyyyfypfy havcyftgci
      361 tffavasfkr skmpkvlqat awciavacgm ccifiktvwy rtrdpttefg klsaaffdri
      421 lwsiflawit lscatgrggf lnkflswsaf tplsrlafgv yiihlpfihl clhisrerif
      481 fshfsvvsff fsmlvwsyll syllfifcea ptgrldklif dpqrrktegr ekdeapndns
      541 vqdklpdavv tfrdrkldiw qtssekntsn gilpgncsrt schl
//
LOCUS       XP_077501015            2400 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111864 [Amblyomma americanum].
ACCESSION   XP_077501015
VERSION     XP_077501015.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644889.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2400
                     /product="uncharacterized protein LOC144111864"
                     /calculated_mol_wt=259520
     Region          <1814..>1974
                     /region_name="PTZ00449"
                     /note="104 kDa microneme/rhoptry antigen; Provisional"
                     /db_xref="CDD:185628"
     CDS             1..2400
                     /gene="LOC144111864"
                     /coded_by="XM_077644889.1:12..7214"
                     /db_xref="GeneID:144111864"
ORIGIN      
        1 mgdrrsltsl ppspphggee skvdqfevtl pdlpsislwl pwslqsvasp pepetsspep
       61 vpestpppgp sqptrlleac vqvsreeqle patleepvvp lasfelpqda aegqehgkeq
      121 mafasisvpe pglvlevtse ppqpkrlsea hmqesldeqq dlamrdvkel valsvssklp
      181 hetagmlehv ndtmalasvs apdlrpvpel tptseppqpt qpsgasvqey leeqlepfts
      241 skleqqnlam eaarelmvts gsfelpqeta mqlehaeeam apvaisalnp gllpevspls
      301 easesrhvlv astqaslsee lksaavskme lqapvmeave eltmtsassk lpqdiagmle
      361 hvagpmalga isvqetgldv parseppqta wppdlfmlsq vaesaarcel kqqnlgqegp
      421 vtalpaspel apasvlesva laassepvqp tttallpqla esglsphpea vfeserrpwv
      481 krrirrplse hspatiswpd qttgtetpeg smtatqghkh ptahgrpwlk rrirhlpseh
      541 spatisrldq atgtetpegs mtatqghkhp tahgdgasde rsmlvnmiae mgdprsltpl
      601 ppspphggee skvdqfevtl pklpskspwl awsplsvasp pepetsppep vpestpppep
      661 sqptrlleac vrvsreeqle patleepvvp lasfelpqda aegqehgkeq tafapistpe
      721 pglvlevtse ppqpmrlsea hmqesldeqq dlamrdvkel valsvssklp hetagtlepv
      781 ndtmalasva apdlrpvpev tptseppqpa qpswdsvqey leeqlepfts skleqrnlam
      841 eaarelmvts gsfelpqeta mqlehaeeam apvaisalkp gllpevspls easesrhvlv
      901 astqaslsqe lksaavskme lqapvmeave eltmtsassk lpqdiagmle hvagpmalga
      961 isvqetgldv parseppqta wppdlfmlsq vaesaarcel kqqnlgqegp vtallaspel
     1021 apapvhesvt laassepvqp tttallpqla esglsphpea vfeserrpwv krrirrplse
     1081 hspatiswpd qttgtetpeg smtatqghkh ptahgrpwlk rrirhlpseh spatisrldq
     1141 atgtetpegs mtatqghkhp tahgdgasde rsmlvnmiae mgdprsltpl ppspphggee
     1201 skvdqfevtl pklpskspwl awsplsvasp pepetsppep vpestpppep sqptrlleac
     1261 vrvsreeqle patleepvvp lasfelpqda aegqehgkeq tafapistpe pglvlevtse
     1321 ppqpmrlsea hmqesldeqq dlamrdvkel valsvssklp hetagtlepv ndtmalasva
     1381 apdlrpvpev tptseppqpa qpswdsvqey leeqlepfts skleqrnlam eaarelmvts
     1441 gsfelpqeta mqlehaeeam apvaisalkp gllpevspls easesrhvlv astqaslsqe
     1501 lksaavskme lqapvmeave eltmtsassk lpqdiagmle hvagpmalga isvqetgldv
     1561 parseppqta wppdlfmlsq vaesaarcel kqqnlgqegp vtallaspel apapvhesvt
     1621 laassepvqp tttallpqla efglsphpea vfesephpaa aaspaeqrdl gaelpvvcpm
     1681 tvrtsaaatl tasgtgqlre gtqpaeaahl psrarpsree ssseeaeqsa ttgqecplqp
     1741 qqmgsvlaal maiveklgqi sdnmqlfrne lglwfrpdlh hptelrdrrc ascgqeqssg
     1801 vapllvrgqe vpgqeqhshd emhngrplev apptkrrhsv hqmseggpfn gleeeeevcr
     1861 krsrpeivsl tspltpphsv perlsreasp ipapllskhs rlprpvevqe tspipaprls
     1921 kdsrlprpve vqeasptpas qltdnsclpr pvevqeaspt lapqltensc lprpvevqea
     1981 sptpasqlnd nsclprpvev qeasptlvpr lieksclprp vevqegkree afaymqflqs
     2041 pahaqlgela lseislskev rafldqlrtl aslseqsdsv avlvsntehn tflcavltll
     2101 srcktnlivk fcqgkckapl ftkmenllli amahaapdrq aallfvliar fcsiwqpksl
     2161 lpqaayvrfm falcrslger avardmiwel mrsdrasflv asavgawpgl fdalspgpvr
     2221 nvisyvlhrc psnelsdtll sycktvldkl gplpcfdgnl yplvddllhp lltthecmdt
     2281 clelrkalee icrrspnsil pwvlrkqllp llqsllsklq lplaqqhatc lirllgplwe
     2341 eskkfpkrld plleqmadcl ahcgsplrga iieafkrlpg fqksrwpael sywflhwnrs
//
LOCUS       XP_077501016            2400 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111864 [Amblyomma americanum].
ACCESSION   XP_077501016
VERSION     XP_077501016.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644890.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2400
                     /product="uncharacterized protein LOC144111864"
                     /calculated_mol_wt=259520
     Region          <1814..>1974
                     /region_name="PTZ00449"
                     /note="104 kDa microneme/rhoptry antigen; Provisional"
                     /db_xref="CDD:185628"
     CDS             1..2400
                     /gene="LOC144111864"
                     /coded_by="XM_077644890.1:12..7214"
                     /db_xref="GeneID:144111864"
ORIGIN      
        1 mgdrrsltsl ppspphggee skvdqfevtl pdlpsislwl pwslqsvasp pepetsspep
       61 vpestpppgp sqptrlleac vqvsreeqle patleepvvp lasfelpqda aegqehgkeq
      121 mafasisvpe pglvlevtse ppqpkrlsea hmqesldeqq dlamrdvkel valsvssklp
      181 hetagmlehv ndtmalasvs apdlrpvpel tptseppqpt qpsgasvqey leeqlepfts
      241 skleqqnlam eaarelmvts gsfelpqeta mqlehaeeam apvaisalnp gllpevspls
      301 easesrhvlv astqaslsee lksaavskme lqapvmeave eltmtsassk lpqdiagmle
      361 hvagpmalga isvqetgldv parseppqta wppdlfmlsq vaesaarcel kqqnlgqegp
      421 vtalpaspel apasvlesva laassepvqp tttallpqla esglsphpea vfeserrpwv
      481 krrirrplse hspatiswpd qttgtetpeg smtatqghkh ptahgrpwlk rrirhlpseh
      541 spatisrldq atgtetpegs mtatqghkhp tahgdgasde rsmlvnmiae mgdprsltpl
      601 ppspphggee skvdqfevtl pklpskspwl awsplsvasp pepetsppep vpestpppep
      661 sqptrlleac vrvsreeqle patleepvvp lasfelpqda aegqehgkeq tafapistpe
      721 pglvlevtse ppqpmrlsea hmqesldeqq dlamrdvkel valsvssklp hetagtlepv
      781 ndtmalasva apdlrpvpev tptseppqpa qpswdsvqey leeqlepfts skleqrnlam
      841 eaarelmvts gsfelpqeta mqlehaeeam apvaisalkp gllpevspls easesrhvlv
      901 astqaslsqe lksaavskme lqapvmeave eltmtsassk lpqdiagmle hvagpmalga
      961 isvqetgldv parseppqta wppdlfmlsq vaesaarcel kqqnlgqegp vtallaspel
     1021 apapvhesvt laassepvqp tttallpqla esglsphpea vfeserrpwv krrirrplse
     1081 hspatiswpd qttgtetpeg smtatqghkh ptahgrpwlk rrirhlpseh spatisrldq
     1141 atgtetpegs mtatqghkhp tahgdgasde rsmlvnmiae mgdprsltpl ppspphggee
     1201 skvdqfevtl pklpskspwl awsplsvasp pepetsppep vpestpppep sqptrlleac
     1261 vrvsreeqle patleepvvp lasfelpqda aegqehgkeq tafapistpe pglvlevtse
     1321 ppqpmrlsea hmqesldeqq dlamrdvkel valsvssklp hetagtlepv ndtmalasva
     1381 apdlrpvpev tptseppqpa qpswdsvqey leeqlepfts skleqrnlam eaarelmvts
     1441 gsfelpqeta mqlehaeeam apvaisalkp gllpevspls easesrhvlv astqaslsqe
     1501 lksaavskme lqapvmeave eltmtsassk lpqdiagmle hvagpmalga isvqetgldv
     1561 parseppqta wppdlfmlsq vaesaarcel kqqnlgqegp vtallaspel apapvhesvt
     1621 laassepvqp tttallpqla efglsphpea vfesephpaa aaspaeqrdl gaelpvvcpm
     1681 tvrtsaaatl tasgtgqlre gtqpaeaahl psrarpsree ssseeaeqsa ttgqecplqp
     1741 qqmgsvlaal maiveklgqi sdnmqlfrne lglwfrpdlh hptelrdrrc ascgqeqssg
     1801 vapllvrgqe vpgqeqhshd emhngrplev apptkrrhsv hqmseggpfn gleeeeevcr
     1861 krsrpeivsl tspltpphsv perlsreasp ipapllskhs rlprpvevqe tspipaprls
     1921 kdsrlprpve vqeasptpas qltdnsclpr pvevqeaspt lapqltensc lprpvevqea
     1981 sptpasqlnd nsclprpvev qeasptlvpr lieksclprp vevqegkree afaymqflqs
     2041 pahaqlgela lseislskev rafldqlrtl aslseqsdsv avlvsntehn tflcavltll
     2101 srcktnlivk fcqgkckapl ftkmenllli amahaapdrq aallfvliar fcsiwqpksl
     2161 lpqaayvrfm falcrslger avardmiwel mrsdrasflv asavgawpgl fdalspgpvr
     2221 nvisyvlhrc psnelsdtll sycktvldkl gplpcfdgnl yplvddllhp lltthecmdt
     2281 clelrkalee icrrspnsil pwvlrkqllp llqsllsklq lplaqqhatc lirllgplwe
     2341 eskkfpkrld plleqmadcl ahcgsplrga iieafkrlpg fqksrwpael sywflhwnrs
//
LOCUS       XP_077501017             759 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077501017
VERSION     XP_077501017.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644891.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..759
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=85529
     Region          76..202
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          296..701
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..759
                     /gene="LOC144111865"
                     /coded_by="XM_077644891.1:269..2548"
                     /db_xref="GeneID:144111865"
ORIGIN      
        1 msvqkvvliv vtvllcgclp cclggsgsdg dssasmteqq nvsyvsklke wiasamnras
       61 psltrkllea evsadcsvgl ltlvrgvrnl epwalrlida takypsgala ttrvdlgafd
      121 ecvqtvvrne yghetaraqy cnvvayggnk tdlddliaaa msithprvak frghiyeqri
      181 pimrlgicvl dvcnqdelql makavlpkvl dvtitdcvta lapdmtrtqv iilaflgtlv
      241 llivlgtavd vycsrrkqkg enkgvllsvl tsfsvvsntr mmlqittnks sdaysmrflh
      301 girffsivyi lvghsygaps diwanmvnai vyadnwanif ttvgymsvdc ffflngflla
      361 fvvhrqkrng vivfviavvr rfirtmvpvf fclmclfllp litsgpdaka yfrklndefq
      421 rqwlylllqv qnydfevtvd tpifghlwyl sldfqfflvs lpillllksr priaaaafvl
      481 lsfvgcsvat wqvagnemtp fivpltesit tflrtgfhyy fypfyhavcy fvgcmtyffv
      541 gifkekkisk acqfaawlfa iacglfcifi kvpwyqvkdp ttefgklsla ffdrilwsil
      601 mswvtiacat grggflntfl swsfftplsr lafgvyiihv pfvqlwlhis rerlffshff
      661 vmsffftilv wsfllsyllf iiceaptgkl eklifeplrr rmskeaigvl phadgaehkl
      721 prvaptrrae kvaslensdd kktsngwhye kgnlttchl
//
LOCUS       XP_077501018             759 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077501018
VERSION     XP_077501018.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644892.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..759
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=85529
     Region          76..202
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          296..701
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..759
                     /gene="LOC144111866"
                     /coded_by="XM_077644892.1:258..2537"
                     /db_xref="GeneID:144111866"
ORIGIN      
        1 msvqkvvliv vtvllcgclp cclggsgsdg dssasmteqq nvsyvsklke wiasamnras
       61 psltrkllea evsadcsvgl ltlvrgvrnl epwalrlida takypsgala ttrvdlgafd
      121 ecvqtvvrne yghetaraqy cnvvayggnk tdlddliaaa msithprvak frghiyeqri
      181 pimrlgicvl dvcnqdelql makavlpkvl dvtitdcvta lapdmtrtqv iilaflgtlv
      241 llivlgtavd vycsrrkqkg enkgvllsvl tsfsvvsntr mmlqittnks sdaysmrflh
      301 girffsivyi lvghsygaps diwanmvnai vyadnwanif ttvgymsvdc ffflngflla
      361 fvvhrqkrng vivfviavvr rfirtmvpvf fclmclfllp litsgpdaka yfrklndefq
      421 rqwlylllqv qnydfevtvd tpifghlwyl sldfqfflvs lpillllksr priaaaafvl
      481 lsfvgcsvat wqvagnemtp fivpltesit tflrtgfhyy fypfyhavcy fvgcmtyffv
      541 gifkekkisk acqfaawlfa iacglfcifi kvpwyqvkdp ttefgklsla ffdrilwsil
      601 mswvtiacat grggflntfl swsfftplsr lafgvyiihv pfvqlwlhis rerlffshff
      661 vmsffftilv wsfllsyllf iiceaptgkl eklifeplrr rmskeaigvl phadgaehkl
      721 prvaptrrae kvaslensdd kktsngwhye kgnlttchl
//
LOCUS       XP_077501019             759 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077501019
VERSION     XP_077501019.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644893.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..759
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=85502
     Region          76..202
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          296..701
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..759
                     /gene="LOC144111867"
                     /coded_by="XM_077644893.1:351..2630"
                     /db_xref="GeneID:144111867"
ORIGIN      
        1 msvqkvvliv vtvllcgclp cclggsgsdg dssasmteqq nvsyvsklke wiasamnras
       61 psltrkllea evsadcsvgl ltlvrgvrnl epwalrlida takypsgala ttrvdlgafd
      121 ecvqtvvrne yghetaraqy cnvvayggnk tdlddliaaa msithprvak frghiyeqri
      181 pimrlgicvl dvcnqdelql makavlpkvl dvtitdcvta lapdmtrtqv iilaflgtlv
      241 llivlgtavd vycsrrkqkg enkgvllsvl tsfsvvsntr mmlqittnks sdaysmrflh
      301 girffsivyi lvghsygaps diwanmvnai vyadnwanif ttvgymsvdc ffflsgflla
      361 fvvhrqkrng vivfviavvr rfirtmvpvf fclmclfllp litsgpdaka yfrklndefq
      421 rqwlylllqv qnydfevtvd tpifghlwyl sldfqfflvs lpillllksr priaaaafvl
      481 lsfvgcsvat wqvagnemtp fivpltesit tflrtgfhyy fypfyhavcy fvgcmtyffv
      541 gifkekkisk acqfaawlfa iacglfcifi kvpwyqvkdp ttefgklsla ffdrilwsil
      601 mswvtiacat grggflntfl swsfftplsr lafgvyiihv pfvqlwlhis rerlffshff
      661 vmsffftilv wsfllsyllf iiceaptgkl eklifeplrr rmskeaigvl phadgaehkl
      721 prvaptrrae kvaslensdd kktsngwhye kgnlttchl
//
LOCUS       XP_077501020             759 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077501020
VERSION     XP_077501020.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644894.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..759
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=85469
     Region          76..202
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          296..701
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..759
                     /gene="LOC144111868"
                     /coded_by="XM_077644894.1:260..2539"
                     /db_xref="GeneID:144111868"
ORIGIN      
        1 msvqkvvliv vtvllcgclp cclggsgsdg dssasmteqq nvsyvsklke wiasamnras
       61 psltrkllea evsadcsvgl ltlvrgvrnl epwalrlida takypsgala ttrvdlgafd
      121 ecvqtvvrne yghetaraqy cnvvayggnk tdlddliaaa msithprvak frghiyeqri
      181 pimrlgicvl dvcnqdelql makavlpkvl dvtitdcvta lapdmtrtqv iilaflgtlv
      241 llivlgtavd vycsrrkqkg enkgvllsvl tsfsvvsntr mmlqittnks sdaysmrflh
      301 girffsivyi lvghsygaps diwanmvnai vyadnwanif ttvgymsvdc ffflngflla
      361 fvvhrqkrng vivfviavvr rfirtmvpvf fclmclfllp litsgpdaka yfrklndefq
      421 rqwlylllqv qnydfevtvd tpifghlwyl sldfqfflvs lpillllksr priaaaafvl
      481 lsfvgcsvat wqvagnemtp fivpltesit tflrtgfhyy fypfyhavcy fvgcmtyffv
      541 gifkekkisk acqfaawlfa iacglfcifi kvpwyqvkdp ttefgklsla ffdrilwsil
      601 mswvtiacat grggflntfl swsfftplsr lafgvyiihv pfvqlwlhis rerlfsshff
      661 vmsffftilv wsfllsyllf iiceaptgkl eklifeplrr rmskeaigvl phadgaehkl
      721 prvaptrrae kvaslensdd kktsngwhye kgnlttchl
//
LOCUS       XP_077501022             877 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111869 [Amblyomma americanum].
ACCESSION   XP_077501022
VERSION     XP_077501022.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644896.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..877
                     /product="uncharacterized protein LOC144111869"
                     /calculated_mol_wt=95980
     CDS             1..877
                     /gene="LOC144111869"
                     /coded_by="XM_077644896.1:24..2657"
                     /db_xref="GeneID:144111869"
ORIGIN      
        1 mtsassklpq diagmlehva epmalgaisv qetgldvlvr seppqtawpp dlfmlsqvae
       61 saarcelkqe nlgqeapvta lpaspelapa pvlesvalaa ssepvqpttt allpqlaesg
      121 lsphpeaafe sephpaaaas paeqrdlgae lpvvcpmtvr tsaaatltas gtgqlregtq
      181 paeaahlpsr arpsreesss eeaeqsattg qecplqpqqm gsvlaalmai veklgqisdn
      241 mqlfrnelgl wfrpdlhhpt elrdrrcasc gqeqssgvap llvrgqevpg qeqhshdemh
      301 ngrplevalp tkrrhsvhqm seggpfngle eeeevcrkrs rpeivsltsp ltpphsvper
      361 lsreaspipa prlskhsrlp rpvevqetsp ipaprlskds rlprpvevqe asptlasqlt
      421 dnsclprpve vqeasptlap qltenscllr pvevqeaspt pasqlndnsc lprpvevqea
      481 sptlvprlie ksclprpvev qegkreeafa ymqflqspah aqlgelalse islskevraf
      541 ldqlrtlasl seqsdsvavl vsntehntfl cavltllsrc ktnlivefcq gkckaplftk
      601 menllliama haapdrqaal lfvliarfcs iwqpksllpq aayvrfmfal crslgerava
      661 rdmiwelmrs drasflvasa vgawpglfda lspgpvrnvi syvlhrcpsn elsdtllsyc
      721 ktvldklgpl pcfdgnlypl vddllhpllt thecmdtcle lrkaleeicr qspnsirpwv
      781 lrkqllpllq sllsklqlpl aqqhatclir llgplweesk kfpkgldpll eqmadclahc
      841 gsplrgaiie afkrlpgfqk srwpaelsyw flhwnrs
//
LOCUS       XP_077501023             877 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111869 [Amblyomma americanum].
ACCESSION   XP_077501023
VERSION     XP_077501023.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644897.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..877
                     /product="uncharacterized protein LOC144111869"
                     /calculated_mol_wt=95980
     CDS             1..877
                     /gene="LOC144111869"
                     /coded_by="XM_077644897.1:24..2657"
                     /db_xref="GeneID:144111869"
ORIGIN      
        1 mtsassklpq diagmlehva epmalgaisv qetgldvlvr seppqtawpp dlfmlsqvae
       61 saarcelkqe nlgqeapvta lpaspelapa pvlesvalaa ssepvqpttt allpqlaesg
      121 lsphpeaafe sephpaaaas paeqrdlgae lpvvcpmtvr tsaaatltas gtgqlregtq
      181 paeaahlpsr arpsreesss eeaeqsattg qecplqpqqm gsvlaalmai veklgqisdn
      241 mqlfrnelgl wfrpdlhhpt elrdrrcasc gqeqssgvap llvrgqevpg qeqhshdemh
      301 ngrplevalp tkrrhsvhqm seggpfngle eeeevcrkrs rpeivsltsp ltpphsvper
      361 lsreaspipa prlskhsrlp rpvevqetsp ipaprlskds rlprpvevqe asptlasqlt
      421 dnsclprpve vqeasptlap qltenscllr pvevqeaspt pasqlndnsc lprpvevqea
      481 sptlvprlie ksclprpvev qegkreeafa ymqflqspah aqlgelalse islskevraf
      541 ldqlrtlasl seqsdsvavl vsntehntfl cavltllsrc ktnlivefcq gkckaplftk
      601 menllliama haapdrqaal lfvliarfcs iwqpksllpq aayvrfmfal crslgerava
      661 rdmiwelmrs drasflvasa vgawpglfda lspgpvrnvi syvlhrcpsn elsdtllsyc
      721 ktvldklgpl pcfdgnlypl vddllhpllt thecmdtcle lrkaleeicr qspnsirpwv
      781 lrkqllpllq sllsklqlpl aqqhatclir llgplweesk kfpkgldpll eqmadclahc
      841 gsplrgaiie afkrlpgfqk srwpaelsyw flhwnrs
//
LOCUS       XP_077501024             295 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111870 [Amblyomma americanum].
ACCESSION   XP_077501024
VERSION     XP_077501024.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644898.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..295
                     /product="uncharacterized protein LOC144111870"
                     /calculated_mol_wt=33466
     CDS             1..295
                     /gene="LOC144111870"
                     /coded_by="XM_077644898.1:272..1159"
                     /db_xref="GeneID:144111870"
ORIGIN      
        1 mlhqilspvf fffstddgpl vlapscsepd khlftvqqpl yelppipldi kadiegtalg
       61 evssrtknri vswitahllt idwypgkfye paakalvlky pvlrdrigtg ydswrvaiky
      121 kmgnirksad ndpnveeark kfgrkgnfqd ksglkkqcrv prdtlettve evdgvvaahv
      181 dfmakemkrc npdiqklkvf mettlpsrrk wikinspste elldkypalt lremihqelm
      241 eickvdferk llgfinghgq rlfsilktsc aaqkifsevq kaiadsegda kkctf
//
LOCUS       XP_077501026             634 aa            linear   INV 25-NOV-2025
DEFINITION  DNA mismatch repair protein Msh6-like, partial [Amblyomma
            americanum].
ACCESSION   XP_077501026
VERSION     XP_077501026.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644900.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on the carboxy end.
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..>634
                     /product="DNA mismatch repair protein Msh6-like"
     Region          63..158
                     /region_name="PWWP"
                     /note="PWWP (Pro-Trp-Trp-Pro) domain; cl02554"
                     /db_xref="CDD:470613"
     Site            order(72,75,102)
                     /site_type="other"
                     /note="putative methyl-histone binding site [polypeptide
                     binding]"
                     /db_xref="CDD:438958"
     Site            73..76
                     /site_type="other"
                     /note="PWWP sequence motif"
                     /db_xref="CDD:438958"
     Region          378..>629
                     /region_name="MutS"
                     /note="DNA mismatch repair ATPase MutS [Replication,
                     recombination and repair]; COG0249"
                     /db_xref="CDD:440019"
     Region          382..498
                     /region_name="MutS_I"
                     /note="MutS domain I; pfam01624"
                     /db_xref="CDD:426350"
     CDS             1..634
                     /gene="LOC144111872"
                     /coded_by="XM_077644900.1:136..>2038"
                     /db_xref="GeneID:144111872"
ORIGIN      
        1 makkntlfdy ftknpnpkaa ptntdseasp aidaegkkst spkgksitng tssskggapc
       61 lhdlvwakvv ghpywpalvt appsgkafvr dhakqplvhv rffesppsra wvrirltkpf
      121 sgarhpevpa qwfkpawnkg veqaekalsi dpddrvllfp ayedheeded ssreeetdde
      181 eeeeeggdep srkkrrisss seyddssede fkpgkddyse sdssssgvde ntisdlepep
      241 pektpqkqsk trkqgdamss npssstkspa vrkladfasp ktpqkqsktc kqrdamssnp
      301 ssstkspavr kladfaspka kseaeqaakg gagatglqtw ahlsydflee gkrrdaagrl
      361 pshpefnprt lsvpvkfkak ltpamrqwwd mkvehfdvvl ffkvgkfyel yhmdavigve
      421 elglvfmkge vahtgfpeia ygrysealiq kgykvarvee tetpkmmeer crkmhrptkf
      481 drvvrreicr itskatrtfg vqdgqlcdpe psyllaiahq lesgesgssi sfgvcfldss
      541 vgkfllgqfn ddhfcsrlrt avshyppvrv lyendqslpa clrqvlegpl hsvprealrp
      601 wrefwdaprt lkqlcegnyf kegypealgh fidp
//
LOCUS       XP_077501027             782 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111873 [Amblyomma americanum].
ACCESSION   XP_077501027
VERSION     XP_077501027.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644901.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..782
                     /product="uncharacterized protein LOC144111873"
                     /calculated_mol_wt=87071
     Region          143..275
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     Region          344..>409
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     Region          464..615
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(476,478,480,504..506,548)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133137"
     Site            476..478
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133137"
     Site            476
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133137"
     Site            order(504..507,510..513)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133137"
     CDS             1..782
                     /gene="LOC144111873"
                     /coded_by="XM_077644901.1:1..2349"
                     /db_xref="GeneID:144111873"
ORIGIN      
        1 merlqkkqaa lrqaidriit aattllqepa vqsgeleeqf yllveqseel kavnesiekr
       61 vdlqelnael aavsvfseki svtktrikra lrnqtssedr sttpsvtgal pepnsqssvn
      121 tgiptsqlps attklpklei akfsgdlrsw qrfwnqfest ihknevlsai akfqyltsyl
      181 tgkaaaaieg lslsnenyav aiktlterfg kedviieehm srllgvrpvh nlhdterlrt
      241 lydeiqsgvr slealgvtss tygvllltgl rksipdqlcl eycrkkatak aspqdelqdf
      301 ldflrieves reraqyssrq sqstvlkprd sslkgktpsa avfavkgres qcsfcgaedh
      361 lpqdcvvsvp lemkkeimak errcfkcakr nhratecrsa kwlkcakcsa rhatgvceln
      421 rptpsharnv paplettvqs slqmaqtnst psvllqtaqa waeakenral vrilldggsq
      481 rtfvkeevsr rlklrvvgke rlaihtfgtt kpsiktcnrv elwlrsrhnd niirlealev
      541 peisgdfllp pdasatslaa dqglqladla pgdhrqntgv ciligadryw dvttgkvkrv
      601 sdklvaveti fgwtlqgivs kttattcltt ttgvmrilvt tqqdndvlsr qlksfwelkh
      661 lgivdreatn kedevlknfq etvtyrdgry qvalpwkhna sdladnkgaa aqrlrsltak
      721 llrsddimqa ydqalreylv sghaeevnrn ggsnltsyve rgsyllskrw vlslpsslav
      781 ft
//
LOCUS       XP_077501028             303 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111874 [Amblyomma americanum].
ACCESSION   XP_077501028
VERSION     XP_077501028.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644902.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..303
                     /product="uncharacterized protein LOC144111874"
                     /calculated_mol_wt=33779
     CDS             1..303
                     /gene="LOC144111874"
                     /coded_by="XM_077644902.1:23..934"
                     /db_xref="GeneID:144111874"
ORIGIN      
        1 mklsahldmt tsthiegfvd lgqfsdhrdk ytkadhglvi mfqplvgswt qiigvfassg
       61 nvksqmlski vleatvlceq agllvhyvct dgatwnrsmw hnlgvygngk nvkckvthpc
      121 dedrflhfis dlphldkcvr ntllkqglnt hlgrahwehv stmwkldnks mtlkvapklt
      181 rshiypngfd kmclsfysla rspkdgnvsp dvlesllsve evlaekesla edsvkalpid
      241 amevaadhhs yaerhsdarl iyyisgyvar krvltthcea craaclcdkd niakklpaep
      301 esl
//
LOCUS       XP_077501029            1114 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111876 [Amblyomma americanum].
ACCESSION   XP_077501029
VERSION     XP_077501029.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644903.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 31% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1114
                     /product="uncharacterized protein LOC144111876"
                     /calculated_mol_wt=121432
     Region          <647..1046
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..1114
                     /gene="LOC144111876"
                     /coded_by="XM_077644903.1:29..3373"
                     /db_xref="GeneID:144111876"
ORIGIN      
        1 mteqleassr qecaplekai ikyegtakrl rmdgvqqeqq vkatsdkqrl avqqstkvkm
       61 elkemkqqwe qvegevefck naiprfiell reeqaqtatl hrsyeeeqql pddvleverk
      121 aqasynsrkr fwlkqdkaen csraliygrt wnqaaseapr dqggqrlkrr carlpskhsp
      181 akilrldqat gtetsegsmt apqghkhpta hgdgafders mlinmraemg drrsltplpp
      241 spphggeesk vdqfqvtlpd lpsislwlpw slqsvasppe petsspepvp estpppgpsq
      301 ptrlleacvq vsreeqlepa tleepvvpla sfelpqdaae gqehgkeqma fasisvpepg
      361 lvlevtsepp qpkrlseahm qesldeqqdl amrdvkelva lsvssklphe tagmlehvnd
      421 tmalasvsap dlrpvpeltp tseppqptqp sgasvqeyle eqlepftssk leqqnlamea
      481 arelmvtsgs felpqetamq lehaeeamap vaisalnpgl lpevsplsea sesrhvlvas
      541 tqaslseelk saavakmelq apvmeaveel tmtsassklp qdiagmlehv agpmalgais
      601 vqetgldvpa rseppqtawp pdlfmlsqva esaarcelkq qnlgqegpvt alpaspelap
      661 asvlesvala assepvqptt tallpqlaes glsphpeavf eserrpwvkr rirrplsehs
      721 patiswpdqt tgtetpegsm tatqghkhpt ahgrpwlkrr irhlpsehsp atisrldqat
      781 gtetpegsmt vtqghkhpta hgdgasders mllnmiaemg dprsltplpp spphggeesk
      841 vdqfevtlpe lpskspwlaw splsvaspse petsppepvp estpppessq ptrlleacvq
      901 vsreeqlepa tleepvvpla sfelpqdaae gqehgkeqta fapisipepg lvlevtsepp
      961 qpmrlseahm qesldeqqdf emrdvkelva lsvssklphe tagmlepvnd tmalasvsap
     1021 dlrpvpeltp tseppqptqp swdsvqeyle eqlepftssk reqqnlamea arelmvtsgs
     1081 felpqetamq lehaeeamap vsisalkpgl lpes
//
LOCUS       XP_077501030             259 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111877 [Amblyomma americanum].
ACCESSION   XP_077501030
VERSION     XP_077501030.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644904.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 20% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..259
                     /product="uncharacterized protein LOC144111877"
                     /calculated_mol_wt=28426
     Region          72..198
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     CDS             1..259
                     /gene="LOC144111877"
                     /coded_by="XM_077644904.1:1..780"
                     /db_xref="GeneID:144111877"
ORIGIN      
        1 mppyvvfkrq tlpkkrfsng iivrvqekgw mseeqqnvsy vsklkewias amdraapslt
       61 rklleadvsa ecsiglltlv rgirnlepla lrlidatgky psglltsara nlgafdkcve
      121 tvghnefgqk targqycnvv ayggnktdld dliaaamsit htrvtrfrgh iyeqtilimr
      181 lgicvldvcs qeelhdvvfs vlpetldati kncitglspr ittaqvtila flgtlglviv
      241 lgnavdlyss rrnekekqg
//
LOCUS       XP_077501032            3439 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111878 [Amblyomma americanum].
ACCESSION   XP_077501032
VERSION     XP_077501032.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644906.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..3439
                     /product="uncharacterized protein LOC144111878"
                     /calculated_mol_wt=373625
     Region          <647..1046
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          <1013..1272
                     /region_name="rne"
                     /note="ribonuclease E; Reviewed; PRK10811"
                     /db_xref="CDD:236766"
     Region          <2853..>3013
                     /region_name="PTZ00449"
                     /note="104 kDa microneme/rhoptry antigen; Provisional"
                     /db_xref="CDD:185628"
     CDS             1..3439
                     /gene="LOC144111878"
                     /coded_by="XM_077644906.1:38..10357"
                     /db_xref="GeneID:144111878"
ORIGIN      
        1 mieqleassr qecaplekai ikyegtakrl rmdgvqqeqq vkatsdkqrl avqqstkvkm
       61 elkemkqqwe qvegevefck naiprfiell reeqaqtatl hrsyeeeqql pddvleverk
      121 aqasynsrkr fwlkqdkaen csraliygrt wnqaaseapr dqggqrlkrr carlpskhsp
      181 akilrldqat gtetsegsmt apqghkhpta hgdgasders mlinmraemg drrsltplpp
      241 spphggeesk vdqfevtlpd lpsislwlpw slqsvasppe petsspepvp estpppgpsq
      301 ptrlleacvq vsreeqlepa tleepvvpla sfelpqdaae gqehgkeqma fasisvpepg
      361 lvlevtsepp qpkrlseahm qesldeqqdl amrdvkelva lsvssklphe tagmlehvnd
      421 tmalasvsap dlrpvpeltp tseppqptqp sgasvqeyle eqlepftssk leqqnlamea
      481 arelmvtsgs felpqetamq lehaeeamap vaisalnpgl lpevsplsea sesrhvlvas
      541 tqaslseelk saavskmelq apvmeaveel tmtsassklp qdiagmlehv agpmalgais
      601 vqetgldvpa rseppqtawp pdlfmlsqva esaarcelkq qnlgqegpvt alpaspelap
      661 asvlesvala assepvqptt tallpqlaes glsphpeavf eserrpwvkr rirrplsehs
      721 patiswpdqt tgtetpegsm tatqghkhpt ahgrpwrkrr irhlpsehsp atisrldqat
      781 gtetpegsmt vtqghkhpta hgdgasders mllnmiaemg dprsltplpp spphggeesk
      841 vdqfevtlpe lpskspwlaw splsvaspse petsppepvp estpppessq ptrlleacvq
      901 vsreeqlepa tleepvvpla sfelpqdaae gqehgkeqta fapisipepg lvlevtsepp
      961 qpmrlseahm qesldeqqdf emrdvkelva lsvssklphe tagmlepvnd tmalasvsap
     1021 dlrpvpeltp tseppqptqp swdsvqeyle eqlepftssk reqqnlamea arelmvtsgs
     1081 felpqetamq lehaeeamap vsisalkpgl lpevsplsea seprhvlvas tqaslseelk
     1141 saavskmelq apvmeaveel tmtsassklp qdiagmlehv aepmalgais vqetgldvlv
     1201 rseppqtawp pdlfmlsqva esaarcelkq enlgqeapvt alpaspelap apvlesvala
     1261 assepvqptt tallpqlaes glsphpeaaf esephpaaaa spaeqrdlga elpvvcpmtv
     1321 rtsaaatlta sgtgqlregt qpaeaahlls rarpsreess seeaeqsatt gqecplqpqq
     1381 mgsvlaalma iveklgqisd nmqlfrnelg lwfrpdlhhp telrdrrcas cgqeqssgva
     1441 pllvrgqevp gqeqqstkvk melkemkqqw eqvegevefc knaiprfivl lreeqaqtat
     1501 lhrsyeeeqq lpddvlever kaqasynsrk rfwlkqdkae ncsraliygr twnqaaseap
     1561 rdqggqrlkr rcarlpskhs pakilrldqa tgtetsegsm tapqghkhpt ahgdgasder
     1621 smlinmraem gdrrsltslp pspphggees kvdqfevtlp dlpsislwlp wslqsvaspp
     1681 epetsspepv pestpppgps qptrlleacv qvsreeqlep atleepvvpl asfelpqdaa
     1741 egqehgkeqm afasisvpep glvlevtsep pqpkrlseah mqesldeqqd lamrdvkelv
     1801 alsvscklph etagmlehvn dtmalasvsa pdlrpvpelt ptseppqptq psgasvqeyl
     1861 eeqlepftss kleqqnlame aarelmvtsg sfelpqetam qlehaeeama pvaisalnpg
     1921 llpevsplse asesrhvlva stqaslseel ksaavskmel qapvmeavee ltmtsasskl
     1981 pqdiagmleh vagpmalgai svqetgldvp arseppqtaw ppdlfmlsqv aesaarcelk
     2041 qqnlgqegpv talpaspela pasvlesval aassepvqpt ttallpqlae sglsphpeav
     2101 feserrpwvk rrirrplseh spatiswpdq ttgtetpegs mtatqghkhp tahgrpwlkr
     2161 rirhlpsehs patisrldqa tgtetpegsm tatqghkhpt ahgdgasder smlvnmiaem
     2221 gdprsltplp pspphggees kvdqfevtlp klpskspwla wsplsvaspp epetsppepv
     2281 pestpppeps qptrlleacv rvsreeqlep atleepvvpl asfelpqdaa egqehgkeqt
     2341 afapistpep glvlevtsep pqpmrlseah mqesldeqqd lamrdvkelv alsvssklph
     2401 etagtlepvn dtmalasvaa pdlrpvpevt ptseppqpaq pswdsvqeyl eeqlepftss
     2461 kleqrnlame aarelmvtsg sfelpqetam qlehaeeama pvaisalkpg llpevsplse
     2521 asesrhvlva stqaslsqel ksaavskmel qapvmeavee ltmtsasskl pqdiagmleh
     2581 vagpmalgai svqetgldvp arseppqtaw ppdlfmlsqv aesaarcelk qqnlgqegpv
     2641 tallaspela papvhesvtl aassepvqpt ttallpqlae fglsphpeav fesephpaaa
     2701 aspaeqrdlg aelpvvcpmt vrtsaaatlt asgtgqlreg tqpaeaahlp srarpsrees
     2761 sseeaeqsat tgqecplqpq qmgsvlaalm aiveklgqis dnmqlfrnel glwfrpdlhh
     2821 ptelrdrrca scgqeqssgv apllvrgqev pgqeqhshde mhngrpleva pptkrrhsvh
     2881 qmseggpfng leeeeevcrk rsrpeivslt spltpphsvp erlsreaspi paprlskhsr
     2941 lprpvevqet spipaprlsk dsrlprpvev qeasptpasq ltdnsclprp vevqeasptl
     3001 apqltenscl prpvevqeas ptpasqlndn sclprpvevq easptlvprl ieksclprpv
     3061 evqegkreea faymqflqsp ahaqlgelal seislskevr afldqlrtla slseqsdsva
     3121 vlvsntehnt flcavltlls rcktnlivkf cqgkckaplf tkmenlllia mahaapdrqa
     3181 allfvliarf csiwqpksll pqaayvrfmf alcrslgera vardmiwelm rsdrasflva
     3241 savgawpglf dalspgpvrn visyvlhrcp snelsdtlls ycktvldklg plpcfdgnly
     3301 plvddllhpl ltthecmdtc lelrkaleei crrspnsilp wvlrkqllpl lqsllsklql
     3361 plaqqhatcl irllgplwee skkfpkrldp lleqmadcla hcgsplrgai ieafkrlpgf
     3421 qksrwpaels ywflhwnrs
//
LOCUS       XP_077501033             259 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111879 [Amblyomma americanum].
ACCESSION   XP_077501033
VERSION     XP_077501033.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644907.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 20% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..259
                     /product="uncharacterized protein LOC144111879"
                     /calculated_mol_wt=28426
     Region          72..198
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     CDS             1..259
                     /gene="LOC144111879"
                     /coded_by="XM_077644907.1:1..780"
                     /db_xref="GeneID:144111879"
ORIGIN      
        1 mppyvvfkrq tlpkkrfsng iivrvqekgw mseeqqnvsy vsklkewias amdraapslt
       61 rklleadvsa ecsiglltlv rgirnlepla lrlidatgky psglltsara nlgafdkcve
      121 tvghnefgqk targqycnvv ayggnktdld dliaaamsit htrvtrfrgh iyeqtilimr
      181 lgicvldvcs qeelhdvvfs vlpetldati kncitglspr ittaqvtila flgtlglviv
      241 lgnavdlyss rrnekekqg
//
LOCUS       XP_077501034            2897 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111880 [Amblyomma americanum].
ACCESSION   XP_077501034
VERSION     XP_077501034.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644908.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 11% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2897
                     /product="uncharacterized protein LOC144111880"
                     /calculated_mol_wt=313406
     Region          <676..1075
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          <1042..1301
                     /region_name="rne"
                     /note="ribonuclease E; Reviewed; PRK10811"
                     /db_xref="CDD:236766"
     CDS             1..2897
                     /gene="LOC144111880"
                     /coded_by="XM_077644908.1:128..8821"
                     /db_xref="GeneID:144111880"
ORIGIN      
        1 mededplkeh cewkeeyedf krkvikrhnm ieqleassrq ecaplekaii kyegtakrlr
       61 mdgvqqeqqv katsdkqrla vqqstkvkme lkemkqqweq vegevefckn aiprfiellr
      121 eeqaqtatlh rsyeeeqqlp ddvleverka qasynsrkrf wlkqdkaenc sraliygrtw
      181 nqaaseaprd qggqrlkrrc arlpskhspa kilrldqatg tetsegsmta pqghkhptah
      241 gdgasdersm linmraemgd rrsltplpps pphggeeskv dqfevtlpdl psislwlpws
      301 lqsvasppep etsspepvpe stpppgpsqp trlleacvqv sreeqlepat leepvvplas
      361 felpqdaaeg qehgkeqmaf asisvpepgl vlevtseppq pkrlseahmq esldeqqdla
      421 mrdvkelval svssklphet agmlehvndt malasvsapd lrpvpeltpt seppqptqps
      481 gasvqeylee qlepftsskl eqqnlameaa relmvtsgsf elpqetamql ehaeeamapv
      541 aisalnpgll pevsplseas esrhvlvast qaslseelks aavskmelqa pvmeaveelt
      601 mtsassklpq diagmlehva gpmalgaisv qetgldvpar seppqtawpp dlfmlsqvae
      661 saarcelkqq nlgqegpvta lpaspelapa svlesvalaa ssepvqpttt allpqlaesg
      721 lsphpeavfe serrpwvkrr irrplsehsp atiswpdqtt gtetpegsmt atqghkhpta
      781 hgrpwrkrri rhlpsehspa tisrldqatg tetpegsmtv tqghkhptah gdgasdersm
      841 llnmiaemgd prsltplpps pphggeeskv dqfevtlpel pskspwlaws plsvaspsep
      901 etsppepvpe stpppessqp trlleacvqv sreeqlepat leepvvplas felpqdaaeg
      961 qehgkeqtaf apisipepgl vlevtseppq pmrlseahmq esldeqqdfe mrdvkelval
     1021 svssklphet agmlepvndt malasvsapd lrpvpeltpt seppqptqps wdsvqeylee
     1081 qlepftsskr eqqnlameaa relmvtsgsf elpqetamql ehaeeamapv sisalkpgll
     1141 pevsplseas eprhvlvast qaslseelks aavskmelqa pvmeaveelt mtsassklpq
     1201 diagmlehva epmalgaisv qetgldvlvr seppqtawpp dlfmlsqvae saarcelkqe
     1261 nlgqeapvta lpaspelapa pvlesvalaa ssepvqpttt allpqlaesg lsphpeaafe
     1321 sephpaaaas paeqrdlgae lpvvcpmtvr tsaaatltas gtgqlregtq paeaahlpsr
     1381 arpsreesss eeaeqsattg qecplqpqqm gsvlaalmai veklgqisdn mqlfrnelgl
     1441 wfrpdlhhpt elrdrrcasc gqeqssgvap llvrgqevpg qeqqstkvkm elkemkqqwe
     1501 qvegevefck nasprfivll reeqaqtatl hrsyeeeqql pddvleverk aqasynsrkr
     1561 fwlkqdkaen csraliygrt wnqaaseapr dqggqrlkrr carlpskhsp akilrldqat
     1621 gtetsegsmt apqghkhpta hgdgasders mlinmraemg drrsltslpp spphggeesk
     1681 vdqfevtlpd lpsislwlpw slqsvasppe petsspepvp estpppgpsq ptrlleacvq
     1741 vsreeqlepa tleepvvpla sfelpqdaae gqehgkeqma fasisvpepg lvlevtsepp
     1801 qpkrlseahm qesldeqqdl amrdvkelva lsvssklphe tagmlehvnd tmalasvsap
     1861 dlrpvpeltp tseppqptqp sgasvqeyle eqlepftssk leqqnlamea arelmvtsgs
     1921 felpqetamq lehaeeamap vaisalnpgl lpevsplsea sesrhvlvas tqaslseelk
     1981 saavskmelq apvmeaveel tmtsassklp qdiagmlehv agpmalgais vqetgldvpa
     2041 rseppqtawp pdlfmlsqva esaarcelkq qnlgqegpvt alpaspelap asvlesvala
     2101 assepvqptt tallpqlaes glsphpeavf eserrpwvkr rirrplsehs patiswpdqt
     2161 tgtetpegsm tatqghkhpt ahgrpwlkrr irhlpsehsp atisrldqat gtetpegsmt
     2221 atqghkhpta hgdgasders mlvnmiaemg dprsltplpp spphggeesk vdqfevtlpk
     2281 lpskspwlaw splsvasppe petsppepvp estpppepsq ptrlleacvr vsreeqlepa
     2341 tleepvvpla sfelpqdaae gqehgkeqta fapistpepg lvlevtsepp qpmrlseahm
     2401 qesldeqqdl amrdvkelva lsvssklphe tagtlepvnd tmalasvaap dlrpvpeatp
     2461 tseppqpaqp swdsvqeyle eqlepftssk leqrnlamea arelmvtsgs felpqetamq
     2521 lehaeeamap vaisalkpgl lpevsplsea sesrhvlvas mqaslsqelk saavskmelq
     2581 apvmeaveel tmtsassklp qdiagmlehv agpmalgais vqetgldvpa rseppqtawp
     2641 pdlfmlsqva esaarcelkq qnlgqegpvt allaspelap apvhesvtla assepvqptt
     2701 tallpqlaef glsphpeavf esephpaaaa spaeqrdlga elpvvcpmtv rtsaaatlta
     2761 sgtgqlregt qpaeaahlps rarpsreess seeaeqsatt gqecplqpqq mgsvlaalma
     2821 iveklgqisd nmqlfrnelg lwfrpelhhp telrdrrcas cgqeqssgva pllvrgqevp
     2881 gqeqvgevrn tpssfaf
//
LOCUS       XP_077501035             259 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111881 [Amblyomma americanum].
ACCESSION   XP_077501035
VERSION     XP_077501035.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644909.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 20% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..259
                     /product="uncharacterized protein LOC144111881"
                     /calculated_mol_wt=28426
     Region          72..198
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     CDS             1..259
                     /gene="LOC144111881"
                     /coded_by="XM_077644909.1:1..780"
                     /db_xref="GeneID:144111881"
ORIGIN      
        1 mppyvvfkrq tlpkkrfsng iivrvqekgw mseeqqnvsy vsklkewias amdraapslt
       61 rklleadvsa ecsiglltlv rgirnlepla lrlidatgky psglltsara nlgafdkcve
      121 tvghnefgqk targqycnvv ayggnktdld dliaaamsit htrvtrfrgh iyeqtilimr
      181 lgicvldvcs qeelhdvvfs vlpetldati kncitglspr ittaqvtila flgtlglviv
      241 lgnavdlyss rrnekekqg
//
LOCUS       XP_077501036            1653 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111882 [Amblyomma americanum].
ACCESSION   XP_077501036
VERSION     XP_077501036.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644910.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1653
                     /product="uncharacterized protein LOC144111882"
                     /calculated_mol_wt=180517
     Region          <676..1075
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          <1042..1301
                     /region_name="rne"
                     /note="ribonuclease E; Reviewed; PRK10811"
                     /db_xref="CDD:236766"
     CDS             1..1653
                     /gene="LOC144111882"
                     /coded_by="XM_077644910.1:128..5089"
                     /db_xref="GeneID:144111882"
ORIGIN      
        1 mededplkeh cewkeeyedf krkvikrhnm ieqleassrq ecaplekaii kyegtakrlr
       61 mdgvqqeqqv katsdkqrla vqqstkvkme lkemkqqweq vegevefckn aiprfiellr
      121 eeqaqtatlh rsyeeeqqlp ddvleverka qasynsrkrf wlkqdkaenc sraliygrtw
      181 nqaaseaprd qggqrlkrrc arlpskhspa kilrldqatg tetsegsmta pqghkhptah
      241 gdgasdersm linmraemgd rrsltplpps pphggeeskv dqfevtlpdl psislwlpws
      301 lqsvasppep etsspepvpe stpppgpsqp trlleacvqv sreeqlepat leepvvplas
      361 felpqdaaeg qehgkeqmaf asisvpepgl vlevtseppq pkrlseahmq esldeqqdla
      421 mrdvkelval svssklphet agmlehvndt malasvsapd lrpvpeltpt seppqptqps
      481 gasvqeylee qlepftsskl eqqnlameaa relmvtsgsf elpqetamql ehaeeamapv
      541 aisalnpgll pevsplseas esrhvlvast qaslseelks aavskmelqa pvmeaveelt
      601 mtsassklpq diagmlehva gpmalgaisv qetgldvpar seppqtawpp dlfmlsqvae
      661 saarcelkqq nlgqegpvta lpaspelapa svlesvalaa ssepvqpttt allpqlaesg
      721 lsphpeavfe serrpwvkrr irrplsehsp atiswpdqtt gtetpegsmt atqghkhpta
      781 hgrpwrkrri rhlpsehspa tisrldqatg tetpegsmtv tqghkhptah gdgasdersm
      841 llnmiaemgd prsltplpps pphggeeskv dqfevtlpel pskspwlaws plsvaspsep
      901 etsppepvpe stpppessqp trlleacvqv sreeqlepat leepvvplas felpqdaaeg
      961 qehgkeqtaf apisipepgl vlevtseppq pmrlseahmq esldeqqdfe mrdvkelval
     1021 svssklphet agmlepvndt malasvsapd lrpvpeltpt seppqptqps wdsvqeylee
     1081 qlepftsskr eqqnlameaa relmvtsgsf elpqetamql ehaeeamapv sisalkpgll
     1141 pevsplseas eprhvlvast qaslseelks aavskmelqa pvmeaveelt mtsassklpq
     1201 diagmlehva epmalgaisv qetgldvlvr seppqtawpp dlfmlsqvae saarcelkqe
     1261 nlgqeapvta lpaspelapa pvlesvalaa ssepvqpttt allpqlaesg lsphpeaafe
     1321 sephpaaaas paeqrdlgae lpvvcpmtvr tsaaatltas gtgqlregtq paeaahlpsr
     1381 arpsreesss eeaeqsattg qecplqpqqm gsvlaalmai veklgqisdn mqlfrnelgl
     1441 wfrpdlhhpt elrdrrcasc gqeqssgvap llvrgqevpg qeqqstkvkm elkemkqqwe
     1501 qvegevefck nasprfivll reeqaqtatl hrsyeeeqql pddvleverk aqasynsrkr
     1561 fwlkqdkaen csraliygrt wnqaaseapr dqggqrlkrr carlpskhsp akilrldqat
     1621 gtetsegsmt apqghkhpta hgertcrlya gfv
//
LOCUS       XP_077501037             259 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111883 [Amblyomma americanum].
ACCESSION   XP_077501037
VERSION     XP_077501037.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644911.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 20% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..259
                     /product="uncharacterized protein LOC144111883"
                     /calculated_mol_wt=28426
     Region          72..198
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     CDS             1..259
                     /gene="LOC144111883"
                     /coded_by="XM_077644911.1:1..780"
                     /db_xref="GeneID:144111883"
ORIGIN      
        1 mppyvvfkrq tlpkkrfsng iivrvqekgw mseeqqnvsy vsklkewias amdraapslt
       61 rklleadvsa ecsiglltlv rgirnlepla lrlidatgky psglltsara nlgafdkcve
      121 tvghnefgqk targqycnvv ayggnktdld dliaaamsit htrvtrfrgh iyeqtilimr
      181 lgicvldvcs qeelhdvvfs vlpetldati kncitglspr ittaqvtila flgtlglviv
      241 lgnavdlyss rrnekekqg
//
LOCUS       XP_077501038             834 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111885 [Amblyomma americanum].
ACCESSION   XP_077501038
VERSION     XP_077501038.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644912.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 21% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..834
                     /product="uncharacterized protein LOC144111885"
                     /calculated_mol_wt=91044
     CDS             1..834
                     /gene="LOC144111885"
                     /coded_by="XM_077644912.1:1..2505"
                     /db_xref="GeneID:144111885"
ORIGIN      
        1 mieqleassr qeltddirhv qgldnapada lsrvtaslas aapplnldfa alakvqqhdt
       61 evmdlrrnpq caplekeiik yegtakrlrm dgvqqeqqvk atsdkqrlav qqftkvkmel
      121 kemkqqweqv egevefckna iprfiallre eqaqtatlhr syeeeqqlpd dvleverkaq
      181 asynsrkrfw lkqdkaencs raliyvrtwn qaaseaprdq ggqrlkrrca rlpskhspak
      241 ilrldqatgt etsegsmtap qghkhptahg dgasdersml inmraemgdr rsltplppsp
      301 phggeeskvd qfevtlpdlp sislwlpwsl qsvasppepe tsspepvpes tpppgpsqpt
      361 rlleacvqvs reeqlepatl eepvvplasf elpqdaaegq ehgkeqmafa sisvpepglv
      421 levtseppqp krlseahmqe sldeqqdlam rdvkelvals vssklpheta gmlehvndtm
      481 alasvsvpdl rpvpeltpts eppqptqpsg asvqeyleeq lepftsskle qqnlameaar
      541 elmvtsgsfe lpqetamqle haeeamapva isalnpgllp evsplsease srhvlvastq
      601 aslseelksa avskmelqap vmeaveeltm tsassklpqd iagmlehvag pmalgaisvq
      661 etgldvpars eppqtawppd lfmlsqvaes aarcelkqqn lgqegpvtal paspelapas
      721 vlesvalaas sepvqpttta llpqlaesgl sphpeavfes errpwvkrri rrplsehspa
      781 tiswpdqttg tetpegsmta tqghkhptah aqpignykrs kemedgslel sekt
//
LOCUS       XP_077501039            2482 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111886 [Amblyomma americanum].
ACCESSION   XP_077501039
VERSION     XP_077501039.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644913.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2482
                     /product="uncharacterized protein LOC144111886"
                     /calculated_mol_wt=265466
     CDS             1..2482
                     /gene="LOC144111886"
                     /coded_by="XM_077644913.1:1..7449"
                     /db_xref="GeneID:144111886"
ORIGIN      
        1 maalvtavsr eeqlepatle epvvplasfe lpqdaaegqe hgkeqmafas isvpepglvl
       61 evtseppqpk rlseahmqes ldeqqdlamr dvkelvalsv ssklphetag mlehvndtma
      121 lasvsvpdlr pvpeltptse ppqptqpsga svqeyleeql epftsskleq qnlameaare
      181 lmvtsgsfel pqetamqleh aeeamapvai salnpgllpe vsplseases rhvlvastqa
      241 slseelksaa vskmelqapv meaveeltmt sassklpqdi agmlehvagp malgaisvqe
      301 tgldvparse ppqtawppdl fmlsqvaesa arcelkqqnl gqegpvtalp aspelapasv
      361 lesvalaass epvqptttal lpqlaesgls phpeavfese rrpwvkrrir rplsehspat
      421 iswpdqttgt etpegsmtat qghkhptahg dgasdersml vnmiaemgdp rsltplppsp
      481 phggeeskvd qfevtlpklp skspwlawsp msvasppepe tsppepvpes tpppepsqpt
      541 rlleacvqvs reeqlepatl eepvvplasf elpqdaaegq ehgkeqmafa sisvpepglv
      601 levtseppqp krlseahmqe sldeqqdlam rdvkelvals vssklpheta gmlehvndtm
      661 alasvsapdl rpvpeltpts eppqptqpsg asvqeyleeq lepftsskle qqnlameaar
      721 elmvtsgsfe lpqetamqle haeeamapva isalnpgllp evsplsease srhvlvastq
      781 aslseelksa avskmelqap vmeaveeltm tsassklpqd iagmlehvag pmalgaisvq
      841 etgldvpars eppqtawppd lfmlsqvaes aarcelkqqn lgqegpvaal paspelapas
      901 vlesvalaas sepvqpttta llpqlaesgl sphpeavfes errpwvkrri rrplsehspa
      961 tiswpdqttg tetpegsmta tqghkhptah grpwlkrrir hlpsehspat isrldqatgt
     1021 etpegsmtvt qghkhptahg dgasdersml lnmiaemgdp rsltplppsp phggeeskvd
     1081 qfevtlpelp skspwlawsp lsvaspsepe tsppepvpes tpppessqpt rlleacvqvs
     1141 reeqlepatl eepvvplasf elpqdaaegq ehgkeqtafa pisipepglv levtseppqp
     1201 mrlseahmqe sldeqqdfem rdvkelvals vssklpheta gmlepvndtm alasvsapdl
     1261 rpvpeltpts eppqptqpsw dsvqeyleeq lepftsskre qqnlameaar elmvtsgsfe
     1321 lpqetamqle haeeamapvs isalkpgllp evsplsease prhvlvastq aslseelksa
     1381 avskmelqap vmkaveeltm tsassklpqd iagmlehvae pmalgaisvq etgldvlvrs
     1441 eppqtawppd lfmlsqvaes aarcelkqen lgqeapvtal paspelapap vlesvalaas
     1501 sepvqpttta llpqlaesgl sphpeaafes ephpaaaasp aeqrdlgael pvvcpmtvrt
     1561 saaatltasg tgqlregtqp aeaahlpsra rpsreessse eaeqsattgq ecplqpqqmg
     1621 svlaalmaiv eklgqisdnm qlfrnelglw frpdlhhpte lrdrrcascg qeqssgvapl
     1681 lvrgqevpgq eqncsraliy vrtwnqaase aprdqggqrl krrcarlpsk hspakilrld
     1741 qatgtetseg smtapqghkh ptahgdgasd ersmlinmra emgdrrsltp lppspphgge
     1801 eskvdqfevt lpdlpsislw lpwslqsvas ppepetsspe pvpestpppg psqptrllea
     1861 cvqvsreeql epatleepvv plasfelpqd aaegqehgke qmafasisvp epglvlevts
     1921 eppqpkrlse ahmqesldeq qdlamrdvke lvalsvsskl phetagmleh vndtmalasv
     1981 sapdlrpvpe ltptseppqp tqpsgasvqe yleeqlepft sskleqqnla meaarelmvt
     2041 sgsfelpqet amqlehaeea mapvaisaln pgllpevspl seasesrhvl vastqaslse
     2101 elksaavskm elqapvmeav eeltmtsass klpqdiagml ehvagpmalg aisvqetgld
     2161 vparseppqt awppdlfmls qvaesaarce lkqqnlgqeg pvtalpaspe lapasvlesv
     2221 alaassepvq ptttallpql aesglsphpe avfeserrpw vkrrirrpls ehspatiswp
     2281 dqttgtetpe gsmtatqghk hptahggqrl krrcarlpsk hspakilrld qatgtetseg
     2341 smtapqghkh ptahgdgasd ersmlinmra emgdrrsltp lppspphgge eskvdqfevt
     2401 lpdlpsislw lpwslqsvas ppepetsspe pvpestpppg psqptrllea cvqvsreeql
     2461 epatleepvg afgflrtatg cg
//
LOCUS       XP_077501040            1244 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111887 [Amblyomma americanum].
ACCESSION   XP_077501040
VERSION     XP_077501040.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644914.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1244
                     /product="uncharacterized protein LOC144111887"
                     /calculated_mol_wt=133207
     CDS             1..1244
                     /gene="LOC144111887"
                     /coded_by="XM_077644914.1:5..3739"
                     /db_xref="GeneID:144111887"
ORIGIN      
        1 mqlehaeeam apvaisalnp gllpevspls easesrhvlv astqaslsee lksaavskme
       61 lqapvmeave eltmtsassk lpqdiagmle hvagpmalga isvqetgldv parseppqta
      121 wppdlfmlsq vaesaarcel kqqnlgqegp vtalpaspel apasvlesva laassepvqp
      181 tttallpqla esglsphpea vfeserrpwv krrirrplse hspatiswpd qttgtetpeg
      241 smtatqghkh ptahggqrlk rrcarlpskh spakilrldq atgtetsegs mtapqghkhp
      301 tahgdgasde rsmlinmrae mgdrrsltpl ppspphggee skvdqfevtl pdlpsislwl
      361 pwslqsvasp pepetsspep vpestpppgp sqptrlleac vqvsreeqle patleepvvp
      421 lasfelpqda aegqehgkeq mafasisvpe pglvlevtse ppqpkrlsea hmqesldeqq
      481 dlamrdvkel valsvssklp hetagmlehv ndtmalasvs apdlrpvpel tptseppqpt
      541 qpsgasvqey leeqlepfts skleqqnlam eaarelmvts gsfelpqeta mqlehaeeam
      601 apvaisalnp gllpevspls easesrhvlv astqaslsee lksaavskme lqapvmeave
      661 eltmtsassk lpqdiagmle hvagpmalga isvqetgldv parseppqta wppdlfmlsq
      721 vaesaarcel kqqnlgqegp vaalpaspel apasvlesva laassepvqp tttallpqla
      781 esglsphpea vfeserrpwv krrirrplse hspatiswpd qttgtetpeg smtatqghkh
      841 ptahgrpwlk rrirhlpseh spatisrldq atgtetpegs mtvtqghkhp tahgdgasde
      901 rsmllnmiae mgdprsltpl ppspphggee skvdqfevtl pelpskspwl awsplsvasp
      961 sepetsppep vpestpppes sqptrlleac vqvsreeqle patleepvvp lasfelpqda
     1021 aegqehgkeq tafapisipe pglvlevtse ppqpmrlsea hmqesldeqq dfemrdvkel
     1081 valsvssklp hetagmlepv ndtmalasvs apdlrpvpel tptseppqpt qpswdsvqey
     1141 leeqlepfts skreqqnlam eaarelmvts gsfelpqeta mqlehaeeam apvsisalkp
     1201 gllpevspls easeprhvlv astqaslsee lksaavskme lqpl
//
LOCUS       XP_077501042             863 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111888 [Amblyomma americanum].
ACCESSION   XP_077501042
VERSION     XP_077501042.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644916.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..863
                     /product="uncharacterized protein LOC144111888"
                     /calculated_mol_wt=94567
     Region          <277..>437
                     /region_name="PTZ00449"
                     /note="104 kDa microneme/rhoptry antigen; Provisional"
                     /db_xref="CDD:185628"
     CDS             1..863
                     /gene="LOC144111888"
                     /coded_by="XM_077644916.1:1..2592"
                     /db_xref="GeneID:144111888"
ORIGIN      
        1 mlehvaepra lgaislqetg ldvlvrsepp qtawppdlfm lsqvaesaar celkqenlgq
       61 eapvtalpas pelapapvle svalaassep vqptttallp qlaesglsph peaafeseph
      121 paaaaspaeq rdlgaelpvv cpmtvrtsaa atltasgtgq lregtqpaea ahlpsrarps
      181 reessseeae qsattgqecp lqpqqmgsvl aalmaivekl gqisdnmqlf rnelglwfrp
      241 dlhhptelrd rrcascgqeq ssgvapllvr gqevpgqeqh shdemhngrp levapptkrr
      301 hsvhqmsegg pfngleeeee vcrkrsrpei vsltspltpp hsvperlsre aspipaprls
      361 khsrlprpve vqetspipap rlskdsrlpr pvevqeaspt pasqltdnsc lprpvevqea
      421 sptlapqlte nsclprpvev qeasptpasq lndnsclprp vevqeasptl vprliekscl
      481 prpvevqegk reeafaymqf lqspahaqlg elalseisls kevrafldql rtlaslseqs
      541 dsvavlvsnt ehntflcavl tllsrcktnl ivkfcqgkck aplftkmenl lliamahaap
      601 drqaallfvl iarfcsiwqp ksllpqaayv rfmfalcrsl geravardmi welmrsdras
      661 flvasavgaw pglfdalspg pvrnvisyvl hrcpsnelsd tllsycktvl dklgplpcfd
      721 gnlyplvddl lhplltthec mdtclelrka leeicrrspn silpwvlrkq llpllqslls
      781 klqlplaqqh atclirllgp lweeskkfpk gldplleqma dclahcgspl rgaiieafkr
      841 lpgfqksrwp aelsywflhw nrs
//
LOCUS       XP_077501043             135 aa            linear   INV 25-NOV-2025
DEFINITION  N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D-like
            [Amblyomma americanum].
ACCESSION   XP_077501043
VERSION     XP_077501043.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644917.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 28% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..135
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..135
                     /product="N-acyl-phosphatidylethanolamine-hydrolyzing
                     phospholipase D-like"
                     /calculated_mol_wt=15504
     Region          <16..120
                     /region_name="UlaG"
                     /note="L-ascorbate lactonase UlaG, metallo-beta-lactamase
                     superfamily [Carbohydrate transport and metabolism];
                     COG2220"
                     /db_xref="CDD:441822"
     Region          <16..66
                     /region_name="metallo-hydrolase-like_MBL-fold"
                     /note="mainly hydrolytic enzymes and related proteins
                     which carry out various biological functions; MBL-fold
                     metallohydrolase domain; cl23716"
                     /db_xref="CDD:451500"
     CDS             1..135
                     /gene="LOC144111889"
                     /coded_by="XM_077644917.1:260..667"
                     /db_xref="GeneID:144111889"
ORIGIN      
        1 mltdylgvil fllqvlwggw cvispeyrfy fagdtgycdv fkqigqaygp fdvaaipiga
       61 yeprwfmkyq hvnpeeavli hkdvrsratl avhwgtftla neyyldppvk lresldrhgi
      121 sprefftlkh amfhg
//
LOCUS       XP_077501044             259 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111890 [Amblyomma americanum].
ACCESSION   XP_077501044
VERSION     XP_077501044.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644918.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 20% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..259
                     /product="uncharacterized protein LOC144111890"
                     /calculated_mol_wt=28426
     Region          72..198
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     CDS             1..259
                     /gene="LOC144111890"
                     /coded_by="XM_077644918.1:1..780"
                     /db_xref="GeneID:144111890"
ORIGIN      
        1 mppyvvfkrq tlpkkrfsng iivrvqekgw mseeqqnvsy vsklkewias amdraapslt
       61 rklleadvsa ecsiglltlv rgirnlepla lrlidatgky psglltsara nlgafdkcve
      121 tvghnefgqk targqycnvv ayggnktdld dliaaamsit htrvtrfrgh iyeqtilimr
      181 lgicvldvcs qeelhdvvfs vlpetldati kncitglspr ittaqvtila flgtlglviv
      241 lgnavdlyss rrnekekqg
//
LOCUS       XP_077501045            2443 aa            linear   INV 25-NOV-2025
DEFINITION  ectopic P granules protein 5 homolog [Amblyomma americanum].
ACCESSION   XP_077501045
VERSION     XP_077501045.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644919.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2443
                     /product="ectopic P granules protein 5 homolog"
                     /calculated_mol_wt=272622
     CDS             1..2443
                     /gene="LOC144111891"
                     /coded_by="XM_077644919.1:275..7606"
                     /db_xref="GeneID:144111891"
ORIGIN      
        1 meqvrvkkkp kkkqhsgqpq astssacess nlavtsakld aegavasqsc deksvvyatp
       61 qpsatgdncd easgaapegv dssacidgtd avlpsypaea vteqqddger cpeelnadqv
      121 tetvtlaapk vptapaesld shyafpsssh aaatercqvg dtkapaavav rsddalsrnr
      181 sigklypels eirdnresmr pftedqilml yesawlkark sivaefvtln eerhlnhhel
      241 yrllenylks reqlastqsv vrslqedvaq qeaclwdivp dvvsgkgrca dnkrvtgehy
      301 fqralynnda asamassaqk llkllgekhv lhmhnahtqr vqidyyfqll ldaapfrdmp
      361 kntpvssgtq pqlagsvael rtcisvlyvf lrkavqddvf vsdvqswlrm lgslllrvas
      421 ladhffllnh vvrcppgihk waislvqips pmpwpentdw rqatvgcqql dyalaalati
      481 llpvesraey lqplqsalea aschepwvvl dsggeeddpp svvywrendv vallnqvpfa
      541 gifqhlllvv sedydaarts eqgvlkliav ashlvsifht glktfnhsry kqltkrisrl
      601 irhtvqyvsd hwksfrltqg andtailmrl qieygqfflr avncifysqs tgawqfmavl
      661 pyscvstvml wqllwllhnn yeekvqqthl spseiceqlq ekgqkqsfee klcsmpqsev
      721 yflltsfanm atsregdgra fthvvalevf qitylnrnlr qlfakegrdl lsslaqkepc
      781 vvsvlldaid dhmmalgvma cylmqsmplh lwvpdkqdld vishfllyyp ldspqsqlar
      841 llienlnygf neqgqlslqh clhqqlalll leaynklcvp feaagyvykq vrylsylatg
      901 ttgetstpmg faawvwttlf qlklhafdsn helqlrlvmd etpppdiapd leqcewlhpl
      961 lraskdlrpp alflsvsigt ighyreqvlg tglklvstli lneqhaaavk cighilplfy
     1021 lhqdmllgnq qfledlqrlv ladntylaaa kslvgseqqg tvlkelaamm tclvqqakcw
     1081 glpslpirlw tallfhlpdt pmhraaykpk grenvmhlld vvvqlayfea dcldsvlahf
     1141 tellatlpap tasqsmmrsv fsymlgsqsg swpamvpfqs aptfpwfsla gmlaeaqlpe
     1201 vatawkslls aiadspqvsm evlvkktmqa pldvlllyrw ayqalqtdad hpalpliwqq
     1261 ffslylqrcp ngnsagprff estsyfsllk kmkrrlsela dhsyqrcant deeepsrdll
     1321 erlfkmyrtf slwleeprlq tasidfsalp sqycpdalca iiqgncqlwh elvsleegqr
     1381 klqslqqlpq rpsasrpcrn qqqhceqape eriisrlkty egqmpapsmp mlrpiipavp
     1441 clapqvrelv saqlrvlvge astvvgrldk ltcldhicre ellpilydnv aafvhlsvpc
     1501 dyreeckgpl hlrlqyyear eapdishkie knrtewiatl telqrappha sccaqvqlea
     1561 cltqlvqrhr qatpgtkeal qklgsevffd vvgflhkemv qylptrqfvt lcldmvgeef
     1621 vanradqclp vlqailndps mvghvapffs pgaaneeqya lmyqsvssci ipslynavfv
     1681 mlskfdlpgw lishepskav rqqllvavek alckcgispq apllplvevf hlhlysllqf
     1741 hfpehyafvl amllrgtesc siavttwgvf lqalgycmpd kvvktydeey akhqclltla
     1801 eaggsidtta dhftglrksd astaksglyg rvrpflpvlp rffsviahcy iweklkapsa
     1861 nlqeavnivl rlygpwlelt dgksmclpwl pgdtkdascm adslvtvlaf fhrccqdawn
     1921 vnvlslawqh yfikyvwtnp peyittavqt afatlpwsqf cpstddmrla cklleakysg
     1981 hldflvnvft qvqwkerlde tlqlaaecvv eyhscfaelv vslawhqnms afvattvgpl
     2041 hehewgmlpv elvslvqkkl acncdvrqll kaspgtdktm ldfvatlscm spgtkrdpnk
     2101 qlsfvttlvg lygpaleaae vqdvtmllpq lldqcqmvqg svpvlgrals lldsgrkgsa
     2161 qeqallggll pwlderpghp vllavlaaac tniasvqhlv cvteaclaaf feentaadgg
     2221 waqaaaafrv peltvadfre qcamqgahlt qmcylmhclp qcrspedera lldqladwvs
     2281 qgraggesep kllllwakll tlslrqldfg gspqvvarll gnfcatlgvl gedrdtggll
     2341 galgmgrrst vsvqfrfccr vvaafvaarl tdnaavanqm lsrlqvlqtt kayqpllqei
     2401 qealnivqdt sltlgdslhm iqtlvnffyc ekaylrvlff gtv
//
LOCUS       XP_077501046            2443 aa            linear   INV 25-NOV-2025
DEFINITION  ectopic P granules protein 5 homolog [Amblyomma americanum].
ACCESSION   XP_077501046
VERSION     XP_077501046.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644920.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2443
                     /product="ectopic P granules protein 5 homolog"
                     /calculated_mol_wt=272622
     CDS             1..2443
                     /gene="LOC144111892"
                     /coded_by="XM_077644920.1:275..7606"
                     /db_xref="GeneID:144111892"
ORIGIN      
        1 meqvrvkkkp kkkqhsgqpq astssacess nlavtsakld aegavasqsc deksvvyatp
       61 qpsatgdncd easgaapegv dssacidgtd avlpsypaea vteqqddger cpeelnadqv
      121 tetvtlaapk vptapaesld shyafpsssh aaatercqvg dtkapaavav rsddalsrnr
      181 sigklypels eirdnresmr pftedqilml yesawlkark sivaefvtln eerhlnhhel
      241 yrllenylks reqlastqsv vrslqedvaq qeaclwdivp dvvsgkgrca dnkrvtgehy
      301 fqralynnda asamassaqk llkllgekhv lhmhnahtqr vqidyyfqll ldaapfrdmp
      361 kntpvssgtq pqlagsvael rtcisvlyvf lrkavqddvf vsdvqswlrm lgslllrvas
      421 ladhffllnh vvrcppgihk waislvqips pmpwpentdw rqatvgcqql dyalaalati
      481 llpvesraey lqplqsalea aschepwvvl dsggeeddpp svvywrendv vallnqvpfa
      541 gifqhlllvv sedydaarts eqgvlkliav ashlvsifht glktfnhsry kqltkrisrl
      601 irhtvqyvsd hwksfrltqg andtailmrl qieygqfflr avncifysqs tgawqfmavl
      661 pyscvstvml wqllwllhnn yeekvqqthl spseiceqlq ekgqkqsfee klcsmpqsev
      721 yflltsfanm atsregdgra fthvvalevf qitylnrnlr qlfakegrdl lsslaqkepc
      781 vvsvlldaid dhmmalgvma cylmqsmplh lwvpdkqdld vishfllyyp ldspqsqlar
      841 llienlnygf neqgqlslqh clhqqlalll leaynklcvp feaagyvykq vrylsylatg
      901 ttgetstpmg faawvwttlf qlklhafdsn helqlrlvmd etpppdiapd leqcewlhpl
      961 lraskdlrpp alflsvsigt ighyreqvlg tglklvstli lneqhaaavk cighilplfy
     1021 lhqdmllgnq qfledlqrlv ladntylaaa kslvgseqqg tvlkelaamm tclvqqakcw
     1081 glpslpirlw tallfhlpdt pmhraaykpk grenvmhlld vvvqlayfea dcldsvlahf
     1141 tellatlpap tasqsmmrsv fsymlgsqsg swpamvpfqs aptfpwfsla gmlaeaqlpe
     1201 vatawkslls aiadspqvsm evlvkktmqa pldvlllyrw ayqalqtdad hpalpliwqq
     1261 ffslylqrcp ngnsagprff estsyfsllk kmkrrlsela dhsyqrcant deeepsrdll
     1321 erlfkmyrtf slwleeprlq tasidfsalp sqycpdalca iiqgncqlwh elvsleegqr
     1381 klqslqqlpq rpsasrpcrn qqqhceqape eriisrlkty egqmpapsmp mlrpiipavp
     1441 clapqvrelv saqlrvlvge astvvgrldk ltcldhicre ellpilydnv aafvhlsvpc
     1501 dyreeckgpl hlrlqyyear eapdishkie knrtewiatl telqrappha sccaqvqlea
     1561 cltqlvqrhr qatpgtkeal qklgsevffd vvgflhkemv qylptrqfvt lcldmvgeef
     1621 vanradqclp vlqailndps mvghvapffs pgaaneeqya lmyqsvssci ipslynavfv
     1681 mlskfdlpgw lishepskav rqqllvavek alckcgispq apllplvevf hlhlysllqf
     1741 hfpehyafvl amllrgtesc siavttwgvf lqalgycmpd kvvktydeey akhqclltla
     1801 eaggsidtta dhftglrksd astaksglyg rvrpflpvlp rffsviahcy iweklkapsa
     1861 nlqeavnivl rlygpwlelt dgksmclpwl pgdtkdascm adslvtvlaf fhrccqdawn
     1921 vnvlslawqh yfikyvwtnp peyittavqt afatlpwsqf cpstddmrla cklleakysg
     1981 hldflvnvft qvqwkerlde tlqlaaecvv eyhscfaelv vslawhqnms afvattvgpl
     2041 hehewgmlpv elvslvqkkl acncdvrqll kaspgtdktm ldfvatlscm spgtkrdpnk
     2101 qlsfvttlvg lygpaleaae vqdvtmllpq lldqcqmvqg svpvlgrals lldsgrkgsa
     2161 qeqallggll pwlderpghp vllavlaaac tniasvqhlv cvteaclaaf feentaadgg
     2221 waqaaaafrv peltvadfre qcamqgahlt qmcylmhclp qcrspedera lldqladwvs
     2281 qgraggesep kllllwakll tlslrqldfg gspqvvarll gnfcatlgvl gedrdtggll
     2341 galgmgrrst vsvqfrfccr vvaafvaarl tdnaavanqm lsrlqvlqtt kayqpllqei
     2401 qealnivqdt sltlgdslhm iqtlvnffyc ekaylrvlff gtv
//
LOCUS       XP_077501047            2443 aa            linear   INV 25-NOV-2025
DEFINITION  ectopic P granules protein 5 homolog [Amblyomma americanum].
ACCESSION   XP_077501047
VERSION     XP_077501047.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644921.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2443
                     /product="ectopic P granules protein 5 homolog"
                     /calculated_mol_wt=272764
     CDS             1..2443
                     /gene="LOC144111893"
                     /coded_by="XM_077644921.1:275..7606"
                     /db_xref="GeneID:144111893"
ORIGIN      
        1 meqvrvkkkp kkkqhsgqpq astssacess nlavtsakld aegavasqsc deksvvyatp
       61 qpsatgdncd easgaapegv dssacidgtd avlpsypaea vteqqddger cpeelnadqv
      121 tetvtlaapk vptapaesld shyafpsssh aaatercqvg dtkapaavav rsddalsrnr
      181 sigklypels eirdnresmr pftedqilml yesawlkark sivaefvtln eerhlnhhel
      241 yrllenylks reqlastqsv mrslqedvaq qeaclwdivp dvvsgkgrca dnkrvtgehy
      301 fqralynnda asamassaqk llkllgekhv lhmhnahtqr vqidyyfqll ldaapfrdmp
      361 kntpvssgtq pqlagsvael rtcisvlyvf lrkavqddvf vsdvqswlrm lgslllrvas
      421 ladhffllnh vvrcppgihk waislvqips pmpwpentdw rqatvgcqql dyalaalati
      481 llpvesraey lqplqsalea aschepwvvl dsdgeeddpp svvywrendv vallnqvpfa
      541 gifqhlllvv sedydaarts eqgvlkliav ashlvsifht glktfnhsry kqltkrisrl
      601 irhtvqyvsd hwksfrltqg andtailmrl qieydqfflr avncifysqs tgawqfmavl
      661 pyscvstvml wqllwllhnn yeekvqqthl spseiceqlq ekgqkqsfee klcsmpqsev
      721 yflltsfanm atsregdgra fthvvalevf qitylnrnlr qlfakegrdl lsslaqkepc
      781 vvsvlldaid dhmmalgvma cylmqsmplh lwvpdkqdld vishfllyyp ldspqsqlar
      841 llienlnygf neqgqlslqh clhqqlalll leaynklcvp feaagyvykq vrylsylatg
      901 ttgetstpmg faawvwttlf qlklhafdsn helqlrlvmd etpppdiapd leqcewlhpl
      961 lraskdlrpp alflsvsigt ighyreqvlg tglklvstli lneqhaaavk cighilplfy
     1021 lhqdmllgnq qfledlqrlv ladntylaaa kslvgseqqg tvlkelaamm tclvqqakcw
     1081 glpslpirlw tallfhlpdt pmhraaykpk grenvmhlld vvvqlayfea dcldsvlahf
     1141 tellatlpap tasqsmmrsv fsymlgsqsg swpamvpfqs aptfpwfsla gmlaeaqlpe
     1201 vatawkslls aiadspqvsm evlvkktmqa pldvlllyrw ayqalqtdad hpalpliwqq
     1261 ffslylqrcp ngnsagprff estsyfsllk kmkrrlsela dhsyqrcant deeepsrdll
     1321 erlfkmyrtf slwleeprlq tasidfsalp sqycpdalca iiqgncqlwh elvsleegqr
     1381 klqslqqlpq rpsasrpcrn qqqhceqape eriisrlkty egqmpapsmp mlrpiipavp
     1441 clapqvrelv saqlrvlvge astvvgrldk ltcldhicre ellpilydnv aafvhlsvpc
     1501 dyreeckgpl hlrlqyyear eapdishkie knrtewiatl telqrappha sccaqvqlea
     1561 cltqlvqrhr qatpgtkeal qklgsevffd vvgflhkemv qylptrqfvt lcldmvgeef
     1621 vanradqclp vlqailndps mvghvapffs pgaaneeqya lmyqsvssci ipslynavfv
     1681 mlskfdlpgw lishepskav rqqllvavek alckcgispq apllplvevf hlhlysllqf
     1741 hfpehyafvl amllrgtesc siavttwgvf lqalgycmpd kvvktydeey akhqclltla
     1801 eaggsidtta dhftglrksd astaksglyg rvrpflpvlp rffsviahcy iweklkapsa
     1861 nlqeavnivl rlygpwlelt dgksmclpwl pgdtkdascm adslvtvlaf fprccqdawn
     1921 vnvlsfawqh yfikyvwtnp peyittavqt afatlpwsqf cpstddmrla cklleakysg
     1981 hldflvnvft qvqwkerlde tlqlaaecvv eyhscfaelv vslawhqnms afvattvgpl
     2041 hehewgmlpv elvslvqkkl acncdvrqll kaspgtdktm ldfvatlscm spgtkrdpnk
     2101 qlsfvttlvg lygpaleaae vqdvtmllpq lldqcqmvqg svpvlgrals lldsgrkgsa
     2161 qeqallggll pwlderpghp vllavlaaac tniasvqhlv cvteaclaaf feentaadgg
     2221 waqaaaafrv peltvadfre qcamqgahlt qmcylmhclp qcrspedera lldqladwvs
     2281 qgraggesep kllllwakll tlslrqldfg gspqvvarll gnfcatlgvl gedrdtggll
     2341 galgmgrrst vsvqfrfccr vvaafvaarl tdnaavanqm lsrlqvlqtt kayqpllqei
     2401 qealnivqdt sltlgdslhm iqtlvnffyc ekaylrvlff gtv
//
LOCUS       XP_077501049            2443 aa            linear   INV 25-NOV-2025
DEFINITION  ectopic P granules protein 5 homolog [Amblyomma americanum].
ACCESSION   XP_077501049
VERSION     XP_077501049.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644923.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2443
                     /product="ectopic P granules protein 5 homolog"
                     /calculated_mol_wt=272924
     CDS             1..2443
                     /gene="LOC144111895"
                     /coded_by="XM_077644923.1:255..7586"
                     /db_xref="GeneID:144111895"
ORIGIN      
        1 meqvrvkkkp kkkqhsgqpq astssacess nlavtsakld aegavasqsc deksvvyatp
       61 qpsatgdncd easgaapegv dssacidgtd avlpsypaea vteqqddger cpeelnadqv
      121 tetvtlaapk vptapaesld shyafpsssh aaatercqvg dtkapaavav rsddalsrnr
      181 sigklypels eirdnresmr pftedqilml yesawlkark sivaefvtln eerhlnhhel
      241 yrllenylks reqlastqsv vrslqedvaq qeaclwdivs dvvsgkgrca dnkrvtgehy
      301 fqralynnda asamassaqk llkllgekhv lhmhnahtqr vqidyyfqll ldaapfrdmp
      361 kntpvssgtq pqlagsvael rtcisvlyvf lrkavqddvf vsdvqswlrm lgslllrvas
      421 ladhffllnh vvrcppgihk waislvqips pmpwpentdw rqatvgcqql dyalaalati
      481 llpvesraey lqplqsalea aschepwvvl dsdgeeddpp svvywrendv vallnqvpfa
      541 gifqhlllvv sedydaarts eqgvlkliav ashlvsifht glktfnhsry kqltkrisrl
      601 irhtvqyvsd hwksfrltqv andtailmrl qieydqfflr avncifysqs tgawqfmavl
      661 pyscvstvml wqllwllhnn yeekvqqthl spseiceqlq ekgqkqsfee klcsmpqsev
      721 yflltsfanm atsregdgra fthvvalevf qitylnrnlr qlfakegrdl lsslaqkepc
      781 vvsvlldaid dhmmalgvma cylmqsmplh lwvpdkqdld vishfllyyp ldspqsqlar
      841 llienlnygf neqgqlslqh clhqqlalll leaynklcvp feaagyvykq vrylsylatg
      901 ttgetstpmg faawvwttlf qlklhafdsn helqlrlvmd etpppdiapd leqcewlhpl
      961 lraskdlrpp alflsvsigt ighyreqvlg tglklvstli lneqhaaavk cighilplfy
     1021 lhqdmllgnq qfledlqrlv ladntylaaa kslvgseqqg tvlkelaamm tclvqqakrw
     1081 glpslpirlw tallfhlpdt pmhrtaykpk grenvmhlld vvvqlayfea dcldsvlahf
     1141 tellatlpap tasqsmmrsv fsymlgsqsg swpamvpfqs aptfpwfsla gmlaeaqlpe
     1201 vatawkslls aiadspqvsm evlvkktmqa pldvlllyrw ayqalqtdad hpalpliwqq
     1261 ffslylqrcp ngnsagprff estsyfsllk kmkrrlsela dhsyqrcant deeepsrdll
     1321 erlfkmyrtf slwleeprlq tasidfralp sqycpdalca iiqgncqlwh elvsleegqr
     1381 klqslqqlpq rpsasrpcrn qqqhceqape eriisrlkty egqmpapsmp mlrpiipavp
     1441 clapqvrelv saqlrvlvge astvvgrldk ltcldhicre ellpilydnv aafvhlsvpc
     1501 dyreeckgpl hlrlqyyear eapdishkie knrtewiatl telqrappha sfcaqvqlea
     1561 cltqlvqrhr qatpgtkeal qklgsevffd vvgflhkemv qylptrqfvt lcldmvgeef
     1621 vanradqclp vlqailndps mvghaapffs pgaaneeqya lmyqsvssci ipslynavfv
     1681 mlskfdlpgw lishepskav rqqllvavek alckcgispq apllplvevf hlhlysllqf
     1741 hfpehyafvl amllrgtesc siavttwgvf lqalgycmpd kvvktydeey akhqclltla
     1801 eaggsidtta dhftglrksd astaksglyg rvrpflpvlp rffsviahcy iweklkapsa
     1861 nlqeavnivl rlygpwlelt dgksmclpwl pgdtkdascm adslvtvlaf fhrccqdawn
     1921 vnvlslawqh yfikyvwtnp peyittavqt afatlpwsqf cpstddmrla cklleakysg
     1981 hldflvnvft qvqwkerlde tlqlaaecvv eyhscfaelv vslawhqnms afvattvgpl
     2041 hehewgmlpv elvslvqkkl acncdvrqll kaspgtdktm ldfvatlscm spgtkrdpnk
     2101 qlsfvttlvg lygpaleaae vqdvtmllpq lldqcqmvqg svpvlgrals lldsgrkgsa
     2161 qeqallggll pwlderpghp vllavlaaac tniasvqhlv cvteaclaaf feentaadgg
     2221 waqaaaafrv peltvadfre qcamqgahlt qmcylmhclp qcrspedera lldqladwvs
     2281 qgraggesep kllllwskll tlslrqldfg gspqvvarll gnfcatlgvl gedrdtggll
     2341 galgmgrrst vsvqfrfccr vvaafvaarl tdnaavanqm lsrlqvlqat kayqpllqei
     2401 qealnivqdt sltlgdslhm iqtlvnffyc ekaylrvlff gtv
//
LOCUS       XP_077501050             759 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077501050
VERSION     XP_077501050.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644924.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..759
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=85481
     Region          76..202
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          296..701
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..759
                     /gene="LOC144111896"
                     /coded_by="XM_077644924.1:149..2428"
                     /db_xref="GeneID:144111896"
ORIGIN      
        1 msvqkvvliv vtvllcgclp cclggsgsdg dssasmteqq nvsyvsklke wiasamnras
       61 psltrkllea evsadcsvgl ltlvrgvrnl epwalrlida takypsgala ttrvdlgafd
      121 ecvqtvvrne yghetaraqy cnvvayggnk tdlddliaaa msithprvak frghiyeqri
      181 pimrlgicvl dvcnqdelql makavlpkvl dvtitdcvta lapdmtrtqv iilaflgtlv
      241 llivlgtavd vycsrrkqkg enkgvllsvl tsfsvvsntr mmlqittnks sdaysmrflh
      301 girffsivyi lvghsygaps diwanmvnai vyadnwanif ttvgymsvdc ffflngflla
      361 fvvhrqkrng vivfviavvr rfirtmvpvf fclmclfllp litsgpdaka yfrklndefq
      421 rqwlylllqv qnydfevtvd tpifghlwyl sldfqfflvs lpillllksr priaaaafvl
      481 lsfvgcsvat wqvagnemtp fivpltesit tflrtgfhyy fypfyhavcy fvgcmtyfvv
      541 gifkekkisk acqfaawlfa iacglfcifi kvpwyqvkdp ttefgklsla ffdrilwsil
      601 mswvtiacat grggflntfl swsfftplsr lafgvyiihv pfvqlwlhis rerlffshff
      661 vmsffftilv wsfllsyllf iiceaptgkl eklifeplrr rmskeaigvl phadgaehkl
      721 prvaptrrae kvaslensdd kktsngwhye kgnlttchl
//
LOCUS       XP_077501051             696 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 1-like isoform
            X1 [Amblyomma americanum].
ACCESSION   XP_077501051
VERSION     XP_077501051.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644925.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..696
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 1-like isoform X1"
                     /calculated_mol_wt=79731
     Region          <23..>106
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          23..50
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(24..25,28..30,32..33,37,40,50,52,54,58..59,62..64,
                     66..67,71,74,83,85,87,91,94,96..97,101,104)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          52..83
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          113..229
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          278..393
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          392..>447
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     Region          <464..617
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..696
                     /gene="LOC144111897"
                     /coded_by="XM_077644925.1:116..2206"
                     /db_xref="GeneID:144111897"
ORIGIN      
        1 mrlekdalia eshdglrrnt lelflscrtg dlarvkylve eqeselnvrd rwdgtplyya
       61 clcghkdvve yllsqgascv antfdgercl yaslsleird llrdrkvits ntmrrdtyde
      121 flrrcledsq hcdvsfsvqg eiipahrcvl aarcdffrrs lldkwagrql ipvthhavdg
      181 avfriitqyl ytgrlemqak hfesflelas rcqlisliad vkealaevln qgsqrsgvsa
      241 esetillept hyreqlqrdf stlpvelasg vtaggaafpa eehnhadici nvdgthflch
      301 kvflcnrsey fraliedhft easrlgsdqq qlpvielqqv tpnifgcvlr hvysnsddrv
      361 natnvwdvlc aadvyllpdl krqcgaqiar lldvesicgt lrasrlfrlp rlenqciefm
      421 akhlaeviel pefheaired akevklrsfl kthfyyyfyp fyhavcyftg citffavasf
      481 krskmpkvlq atawciavac gmccifmktv wyrtrdptte fgklsaaffd rilwsiflaw
      541 itlscatgrg gflnkflsws aftplsrlaf gvyiihlpfi hlclhisrer iffshfsvvs
      601 fffsmlvwsy llsyllfifc evptgrldkl ifdpqrrkte grekdeapnd nsvqdklpda
      661 vvtfrdrkld iwqtsseknt sngilpgncs rtschl
//
LOCUS       XP_077501052             673 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 1-like isoform
            X2 [Amblyomma americanum].
ACCESSION   XP_077501052
VERSION     XP_077501052.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644926.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..673
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 1-like isoform X2"
                     /calculated_mol_wt=76894
     Region          <23..>106
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          23..50
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(24..25,28..30,32..33,37,40,50,52,54,58..59,62..64,
                     66..67,71,74,83,85,87,91,94,96..97,101,104)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          52..83
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          113..229
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          278..393
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          392..>447
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     Region          <464..582
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; cl21495"
                     /db_xref="CDD:473885"
     CDS             1..673
                     /gene="LOC144111897"
                     /coded_by="XM_077644926.1:116..2137"
                     /db_xref="GeneID:144111897"
ORIGIN      
        1 mrlekdalia eshdglrrnt lelflscrtg dlarvkylve eqeselnvrd rwdgtplyya
       61 clcghkdvve yllsqgascv antfdgercl yaslsleird llrdrkvits ntmrrdtyde
      121 flrrcledsq hcdvsfsvqg eiipahrcvl aarcdffrrs lldkwagrql ipvthhavdg
      181 avfriitqyl ytgrlemqak hfesflelas rcqlisliad vkealaevln qgsqrsgvsa
      241 esetillept hyreqlqrdf stlpvelasg vtaggaafpa eehnhadici nvdgthflch
      301 kvflcnrsey fraliedhft easrlgsdqq qlpvielqqv tpnifgcvlr hvysnsddrv
      361 natnvwdvlc aadvyllpdl krqcgaqiar lldvesicgt lrasrlfrlp rlenqciefm
      421 akhlaeviel pefheaired akevklrsfl kthfyyyfyp fyhavcyftg citffavasf
      481 krskmpkvlq atawciavac gmccifmktv wyrtrdptte fgklsaaffd rilwsiflaw
      541 itlscatgrg gflnkflsws aftplsrlaf gvyiihlpfi hlclhisrer iffshfsvvp
      601 tgrldklifd pqrrktegre kdeapndnsv qdklpdavvt frdrkldiwq tssekntsng
      661 ilpgncsrts chl
//
LOCUS       XP_077501053             638 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 1-like isoform
            X3 [Amblyomma americanum].
ACCESSION   XP_077501053
VERSION     XP_077501053.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644927.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..638
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 1-like isoform X3"
                     /calculated_mol_wt=72992
     Region          <23..>106
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          23..50
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(24..25,28..30,32..33,37,40,50,52,54,58..59,62..64,
                     66..67,71,74,83,85,87,91,94,96..97,101,104)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          52..83
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          113..229
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          278..335
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          334..>389
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     Region          <406..559
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..638
                     /gene="LOC144111897"
                     /coded_by="XM_077644927.1:116..2032"
                     /db_xref="GeneID:144111897"
ORIGIN      
        1 mrlekdalia eshdglrrnt lelflscrtg dlarvkylve eqeselnvrd rwdgtplyya
       61 clcghkdvve yllsqgascv antfdgercl yaslsleird llrdrkvits ntmrrdtyde
      121 flrrcledsq hcdvsfsvqg eiipahrcvl aarcdffrrs lldkwagrql ipvthhavdg
      181 avfriitqyl ytgrlemqak hfesflelas rcqlisliad vkealaevln qgsqrsgvsa
      241 esetillept hyreqlqrdf stlpvelasg vtaggaafpa eehnhadici nvdgthflch
      301 kvnatnvwdv lcaadvyllp dlkrqcgaqi arlldvesic gtlrasrlfr lprlenqcie
      361 fmakhlaevi elpefheair edakevklrs flkthfyyyf ypfyhavcyf tgcitffava
      421 sfkrskmpkv lqatawciav acgmccifmk tvwyrtrdpt tefgklsaaf fdrilwsifl
      481 awitlscatg rggflnkfls wsaftplsrl afgvyiihlp fihlclhisr eriffshfsv
      541 vsfffsmlvw syllsyllfi fcevptgrld klifdpqrrk tegrekdeap ndnsvqdklp
      601 davvtfrdrk ldiwqtssek ntsngilpgn csrtschl
//
LOCUS       XP_077501054             584 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 1-like isoform
            X4 [Amblyomma americanum].
ACCESSION   XP_077501054
VERSION     XP_077501054.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644928.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..584
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 1-like isoform X4"
                     /calculated_mol_wt=66932
     Region          1..117
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          166..281
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          280..>335
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     Region          <352..505
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..584
                     /gene="LOC144111897"
                     /coded_by="XM_077644928.1:333..2087"
                     /db_xref="GeneID:144111897"
ORIGIN      
        1 mrrdtydefl rrcledsqhc dvsfsvqgei ipahrcvlaa rcdffrrsll dkwagrqlip
       61 vthhavdgav friitqylyt grlemqakhf esflelasrc qlisliadvk ealaevlnqg
      121 sqrsgvsaes etillepthy reqlqrdfst lpvelasgvt aggaafpaee hnhadicinv
      181 dgthflchkv flcnrseyfr aliedhftea srlgsdqqql pvielqqvtp nifgcvlrhv
      241 ysnsddrvna tnvwdvlcaa dvyllpdlkr qcgaqiarll dvesicgtlr asrlfrlprl
      301 enqciefmak hlaevielpe fheairedak evklrsflkt hfyyyfypfy havcyftgci
      361 tffavasfkr skmpkvlqat awciavacgm ccifmktvwy rtrdpttefg klsaaffdri
      421 lwsiflawit lscatgrggf lnkflswsaf tplsrlafgv yiihlpfihl clhisrerif
      481 fshfsvvsff fsmlvwsyll syllfifcev ptgrldklif dpqrrktegr ekdeapndns
      541 vqdklpdavv tfrdrkldiw qtssekntsn gilpgncsrt schl
//
LOCUS       XP_077501055             561 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 1-like isoform
            X5 [Amblyomma americanum].
ACCESSION   XP_077501055
VERSION     XP_077501055.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644929.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..561
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 1-like isoform X5"
                     /calculated_mol_wt=64095
     Region          1..117
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          166..281
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          280..>335
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     Region          <352..470
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; cl21495"
                     /db_xref="CDD:473885"
     CDS             1..561
                     /gene="LOC144111897"
                     /coded_by="XM_077644929.1:333..2018"
                     /db_xref="GeneID:144111897"
ORIGIN      
        1 mrrdtydefl rrcledsqhc dvsfsvqgei ipahrcvlaa rcdffrrsll dkwagrqlip
       61 vthhavdgav friitqylyt grlemqakhf esflelasrc qlisliadvk ealaevlnqg
      121 sqrsgvsaes etillepthy reqlqrdfst lpvelasgvt aggaafpaee hnhadicinv
      181 dgthflchkv flcnrseyfr aliedhftea srlgsdqqql pvielqqvtp nifgcvlrhv
      241 ysnsddrvna tnvwdvlcaa dvyllpdlkr qcgaqiarll dvesicgtlr asrlfrlprl
      301 enqciefmak hlaevielpe fheairedak evklrsflkt hfyyyfypfy havcyftgci
      361 tffavasfkr skmpkvlqat awciavacgm ccifmktvwy rtrdpttefg klsaaffdri
      421 lwsiflawit lscatgrggf lnkflswsaf tplsrlafgv yiihlpfihl clhisrerif
      481 fshfsvvptg rldklifdpq rrktegrekd eapndnsvqd klpdavvtfr drkldiwqts
      541 sekntsngil pgncsrtsch l
//
LOCUS       XP_077501056             759 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077501056
VERSION     XP_077501056.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644930.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..759
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=85481
     Region          76..202
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          296..701
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..759
                     /gene="LOC144111899"
                     /coded_by="XM_077644930.1:149..2428"
                     /db_xref="GeneID:144111899"
ORIGIN      
        1 msvqkvvliv vtvllcgclp cclggsgsdg dssasmteqq nvsyvsklke wiasamnras
       61 psltrkllea evsadcsvgl ltlvrgvrnl epwalrlida takypsgala ttrvdlgafd
      121 ecvqtvvrne yghetaraqy cnvvayggnk tdlddliaaa msithprvak frghiyeqri
      181 pimrlgicvl dvcnqdelql makavlpkvl dvtitdcvta lapdmtrtqv iilaflgtlv
      241 llivlgtavd vycsrrkqkg enkgvllsvl tsfsvvsntr mmlqittnks sdaysmrflh
      301 girffsivyi lvghsygaps diwanmvnai vyadnwanif ttvgymsvdc ffflngflla
      361 fvvhrqkrng vivfviavvr rfirtmvpvf fclmclfllp litsgpdaka yfrklndefq
      421 rqwlylllqv qnydfevtvd tpifghlwyl sldfqfflvs lpillllksr priaaaafvl
      481 lsfvgcsvat wqvagnemtp fivpltesit tflrtgfhyy fypfyhavcy fvgcmtyfvv
      541 gifkekkisk acqfaawlfa iacglfcifi kvpwyqvkdp ttefgklsla ffdrilwsil
      601 mswvtiacat grggflntfl swsfftplsr lafgvyiihv pfvqlwlhis rerlffshff
      661 vmsffftilv wsfllsyllf iiceaptgkl eklifeplrr rmskeaigvl phadgaehkl
      721 prvaptrrae kvaslensdd kktsngwhye kgnlttchl
//
LOCUS       XP_077501058             696 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 1-like isoform
            X1 [Amblyomma americanum].
ACCESSION   XP_077501058
VERSION     XP_077501058.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644932.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..696
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 1-like isoform X1"
                     /calculated_mol_wt=79731
     Region          <23..>106
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          23..50
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(24..25,28..30,32..33,37,40,50,52,54,58..59,62..64,
                     66..67,71,74,83,85,87,91,94,96..97,101,104)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          52..83
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          113..229
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          278..393
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          392..>447
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     Region          <464..617
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..696
                     /gene="LOC144111900"
                     /coded_by="XM_077644932.1:116..2206"
                     /db_xref="GeneID:144111900"
ORIGIN      
        1 mrlekdalia eshdglrrnt lelflscrtg dlarvkylve eqeselnvrd rwdgtplyya
       61 clcghkdvve yllsqgascv antfdgercl yaslsleird llrdrkvits ntmrrdtyde
      121 flrrcledsq hcdvsfsvqg eiipahrcvl aarcdffrrs lldkwagrql ipvthhavdg
      181 avfriitqyl ytgrlemqak hfesflelas rcqlisliad vkealaevln qgsqrsgvsa
      241 esetillept hyreqlqrdf stlpvelasg vtaggaafpa eehnhadici nvdgthflch
      301 kvflcnrsey fraliedhft easrlgsdqq qlpvielqqv tpnifgcvlr hvysnsddrv
      361 natnvwdvlc aadvyllpdl krqcgaqiar lldvesicgt lrasrlfrlp rlenqciefm
      421 akhlaeviel pefheaired akevklrsfl kthfyyyfyp fyhavcyftg citffavasf
      481 krskmpkvlq atawciavac gmccifmktv wyrtrdptte fgklsaaffd rilwsiflaw
      541 itlscatgrg gflnkflsws aftplsrlaf gvyiihlpfi hlclhisrer iffshfsvvs
      601 fffsmlvwsy llsyllfifc evptgrldkl ifdpqrrkte grekdeapnd nsvqdklpda
      661 vvtfrdrkld iwqtsseknt sngilpgncs rtschl
//
LOCUS       XP_077501059             673 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 1-like isoform
            X2 [Amblyomma americanum].
ACCESSION   XP_077501059
VERSION     XP_077501059.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644933.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..673
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 1-like isoform X2"
                     /calculated_mol_wt=76894
     Region          <23..>106
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          23..50
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(24..25,28..30,32..33,37,40,50,52,54,58..59,62..64,
                     66..67,71,74,83,85,87,91,94,96..97,101,104)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          52..83
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          113..229
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          278..393
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          392..>447
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     Region          <464..582
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; cl21495"
                     /db_xref="CDD:473885"
     CDS             1..673
                     /gene="LOC144111900"
                     /coded_by="XM_077644933.1:116..2137"
                     /db_xref="GeneID:144111900"
ORIGIN      
        1 mrlekdalia eshdglrrnt lelflscrtg dlarvkylve eqeselnvrd rwdgtplyya
       61 clcghkdvve yllsqgascv antfdgercl yaslsleird llrdrkvits ntmrrdtyde
      121 flrrcledsq hcdvsfsvqg eiipahrcvl aarcdffrrs lldkwagrql ipvthhavdg
      181 avfriitqyl ytgrlemqak hfesflelas rcqlisliad vkealaevln qgsqrsgvsa
      241 esetillept hyreqlqrdf stlpvelasg vtaggaafpa eehnhadici nvdgthflch
      301 kvflcnrsey fraliedhft easrlgsdqq qlpvielqqv tpnifgcvlr hvysnsddrv
      361 natnvwdvlc aadvyllpdl krqcgaqiar lldvesicgt lrasrlfrlp rlenqciefm
      421 akhlaeviel pefheaired akevklrsfl kthfyyyfyp fyhavcyftg citffavasf
      481 krskmpkvlq atawciavac gmccifmktv wyrtrdptte fgklsaaffd rilwsiflaw
      541 itlscatgrg gflnkflsws aftplsrlaf gvyiihlpfi hlclhisrer iffshfsvvp
      601 tgrldklifd pqrrktegre kdeapndnsv qdklpdavvt frdrkldiwq tssekntsng
      661 ilpgncsrts chl
//
LOCUS       XP_077501060             638 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 1-like isoform
            X3 [Amblyomma americanum].
ACCESSION   XP_077501060
VERSION     XP_077501060.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644934.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..638
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 1-like isoform X3"
                     /calculated_mol_wt=72992
     Region          <23..>106
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          23..50
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(24..25,28..30,32..33,37,40,50,52,54,58..59,62..64,
                     66..67,71,74,83,85,87,91,94,96..97,101,104)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          52..83
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          113..229
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          278..335
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          334..>389
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     Region          <406..559
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..638
                     /gene="LOC144111900"
                     /coded_by="XM_077644934.1:116..2032"
                     /db_xref="GeneID:144111900"
ORIGIN      
        1 mrlekdalia eshdglrrnt lelflscrtg dlarvkylve eqeselnvrd rwdgtplyya
       61 clcghkdvve yllsqgascv antfdgercl yaslsleird llrdrkvits ntmrrdtyde
      121 flrrcledsq hcdvsfsvqg eiipahrcvl aarcdffrrs lldkwagrql ipvthhavdg
      181 avfriitqyl ytgrlemqak hfesflelas rcqlisliad vkealaevln qgsqrsgvsa
      241 esetillept hyreqlqrdf stlpvelasg vtaggaafpa eehnhadici nvdgthflch
      301 kvnatnvwdv lcaadvyllp dlkrqcgaqi arlldvesic gtlrasrlfr lprlenqcie
      361 fmakhlaevi elpefheair edakevklrs flkthfyyyf ypfyhavcyf tgcitffava
      421 sfkrskmpkv lqatawciav acgmccifmk tvwyrtrdpt tefgklsaaf fdrilwsifl
      481 awitlscatg rggflnkfls wsaftplsrl afgvyiihlp fihlclhisr eriffshfsv
      541 vsfffsmlvw syllsyllfi fcevptgrld klifdpqrrk tegrekdeap ndnsvqdklp
      601 davvtfrdrk ldiwqtssek ntsngilpgn csrtschl
//
LOCUS       XP_077501061             584 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 1-like isoform
            X4 [Amblyomma americanum].
ACCESSION   XP_077501061
VERSION     XP_077501061.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644935.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..584
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 1-like isoform X4"
                     /calculated_mol_wt=66932
     Region          1..117
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          166..281
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          280..>335
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     Region          <352..505
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..584
                     /gene="LOC144111900"
                     /coded_by="XM_077644935.1:333..2087"
                     /db_xref="GeneID:144111900"
ORIGIN      
        1 mrrdtydefl rrcledsqhc dvsfsvqgei ipahrcvlaa rcdffrrsll dkwagrqlip
       61 vthhavdgav friitqylyt grlemqakhf esflelasrc qlisliadvk ealaevlnqg
      121 sqrsgvsaes etillepthy reqlqrdfst lpvelasgvt aggaafpaee hnhadicinv
      181 dgthflchkv flcnrseyfr aliedhftea srlgsdqqql pvielqqvtp nifgcvlrhv
      241 ysnsddrvna tnvwdvlcaa dvyllpdlkr qcgaqiarll dvesicgtlr asrlfrlprl
      301 enqciefmak hlaevielpe fheairedak evklrsflkt hfyyyfypfy havcyftgci
      361 tffavasfkr skmpkvlqat awciavacgm ccifmktvwy rtrdpttefg klsaaffdri
      421 lwsiflawit lscatgrggf lnkflswsaf tplsrlafgv yiihlpfihl clhisrerif
      481 fshfsvvsff fsmlvwsyll syllfifcev ptgrldklif dpqrrktegr ekdeapndns
      541 vqdklpdavv tfrdrkldiw qtssekntsn gilpgncsrt schl
//
LOCUS       XP_077501062             561 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 1-like isoform
            X5 [Amblyomma americanum].
ACCESSION   XP_077501062
VERSION     XP_077501062.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644936.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..561
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 1-like isoform X5"
                     /calculated_mol_wt=64095
     Region          1..117
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          166..281
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          280..>335
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     Region          <352..470
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; cl21495"
                     /db_xref="CDD:473885"
     CDS             1..561
                     /gene="LOC144111900"
                     /coded_by="XM_077644936.1:333..2018"
                     /db_xref="GeneID:144111900"
ORIGIN      
        1 mrrdtydefl rrcledsqhc dvsfsvqgei ipahrcvlaa rcdffrrsll dkwagrqlip
       61 vthhavdgav friitqylyt grlemqakhf esflelasrc qlisliadvk ealaevlnqg
      121 sqrsgvsaes etillepthy reqlqrdfst lpvelasgvt aggaafpaee hnhadicinv
      181 dgthflchkv flcnrseyfr aliedhftea srlgsdqqql pvielqqvtp nifgcvlrhv
      241 ysnsddrvna tnvwdvlcaa dvyllpdlkr qcgaqiarll dvesicgtlr asrlfrlprl
      301 enqciefmak hlaevielpe fheairedak evklrsflkt hfyyyfypfy havcyftgci
      361 tffavasfkr skmpkvlqat awciavacgm ccifmktvwy rtrdpttefg klsaaffdri
      421 lwsiflawit lscatgrggf lnkflswsaf tplsrlafgv yiihlpfihl clhisrerif
      481 fshfsvvptg rldklifdpq rrktegrekd eapndnsvqd klpdavvtfr drkldiwqts
      541 sekntsngil pgncsrtsch l
//
LOCUS       XP_077501063             729 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501063
VERSION     XP_077501063.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644937.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..729
                     /product="nose resistant to fluoxetine protein 6-like
                     isoform X1"
                     /calculated_mol_wt=82214
     Region          75..201
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          295..689
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..729
                     /gene="LOC144111901"
                     /coded_by="XM_077644937.1:176..2365"
                     /db_xref="GeneID:144111901"
ORIGIN      
        1 mrsrkrvisl ltrlffalav viclratscv ahieannqtd vkiveklkew igdaidrapp
       61 sfmrkllead vsskcslgll klvrgvrnle pwalrlldas gkyptgafql sradlgafde
      121 cletvqldey gremvrgqyc nllvyatnns dlndlitsam vfthprvrkf tgslfeqrlp
      181 lvrlgiclld dctqdelqal lvavlplsad vsikncvtat nfaftkgqvl iisflgvlal
      241 vvliatavdi fssqedpkrr nrsallsvlk sfsvasntrm mlhiarekss dacslrflhg
      301 irffsivwiv mghcycspsd vwsrmmnmvl yadqwqsmil tagfigvdsf fflsgyllis
      361 ivwkqkrngv vvflfavvrr lfrtmvpvff llmclqllpl iasgpdskaf fekidedfrr
      421 qwlaillqvq nyafkkdaia pvfghfwyls vdfqfflvtl pillllkkrp kvavgvlall
      481 slvgcsiatw qvaasrktpf ivlvteslst fletgyyyyf ypfyhavcyf tgcitfflvs
      541 rfktrkislp lqaaawcvav scgmgcifmk tawyrtrdpt telgklsaaf fdrilwsvfl
      601 switvacatg rggfvntfls wspftplsrl afgvylihll fiqlflcisr erlffshffa
      661 vslffttivw syllsyllfi cceaptgrld klvfepqskq teksagdaap nangtedglp
      721 qvvaklrda
//
LOCUS       XP_077501064             723 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501064
VERSION     XP_077501064.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644938.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..723
                     /product="nose resistant to fluoxetine protein 6-like
                     isoform X2"
                     /calculated_mol_wt=81634
     Region          75..201
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          289..683
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..723
                     /gene="LOC144111901"
                     /coded_by="XM_077644938.1:177..2348"
                     /db_xref="GeneID:144111901"
ORIGIN      
        1 mrsrkrvisl ltrlffalav viclratscv ahieannqtd vkiveklkew igdaidrapp
       61 sfmrkllead vsskcslgll klvrgvrnle pwalrlldas gkyptgafql sradlgafde
      121 cletvqldey gremvrgqyc nllvyatnns dlndlitsam vfthprvrkf tgslfeqrlp
      181 lvrlgiclld dctqdelqal lvadvsiknc vtatnfaftk gqvliisflg vlalvvliat
      241 avdifssqed pkrrnrsall svlksfsvas ntrmmlhiar ekssdacslr flhgirffsi
      301 vwivmghcyc spsdvwsrmm nmvlyadqwq smiltagfig vdsffflsgy llisivwkqk
      361 rngvvvflfa vvrrlfrtmv pvffllmclq llpliasgpd skaffekide dfrrqwlail
      421 lqvqnyafkk daiapvfghf wylsvdfqff lvtlpillll kkrpkvavgv lallslvgcs
      481 iatwqvaasr ktpfivlvte slstfletgy yyyfypfyha vcyftgcitf flvsrfktrk
      541 islplqaaaw cvavscgmgc ifmktawyrt rdpttelgkl saaffdrilw svflswitva
      601 catgrggfvn tflswspftp lsrlafgvyl ihllfiqlfl cisrerlffs hffavslfft
      661 tivwsyllsy llficceapt grldklvfep qskqteksag daapnangte dglpqvvakl
      721 rda
//
LOCUS       XP_077501065             596 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501065
VERSION     XP_077501065.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644939.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..596
                     /product="nose resistant to fluoxetine protein 6-like
                     isoform X3"
                     /calculated_mol_wt=67324
     Region          162..556
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..596
                     /gene="LOC144111901"
                     /coded_by="XM_077644939.1:3458..5248"
                     /db_xref="GeneID:144111901"
ORIGIN      
        1 mvrgqycnll vyatnnsdln dlitsamvft hprvrkftgs lfeqrlplvr lgicllddct
       61 qdelqallva vlplsadvsi kncvtatnfa ftkgqvliis flgvlalvvl iatavdifss
      121 qedpkrrnrs allsvlksfs vasntrmmlh iarekssdac slrflhgirf fsivwivmgh
      181 cycspsdvws rmmnmvlyad qwqsmiltag figvdsfffl sgyllisivw kqkrngvvvf
      241 lfavvrrlfr tmvpvffllm clqllplias gpdskaffek idedfrrqwl aillqvqnya
      301 fkkdaiapvf ghfwylsvdf qfflvtlpil lllkkrpkva vgvlallslv gcsiatwqva
      361 asrktpfivl vteslstfle tgyyyyfypf yhavcyftgc itfflvsrfk trkislplqa
      421 aawcvavscg mgcifmktaw yrtrdpttel gklsaaffdr ilwsvflswi tvacatgrgg
      481 fvntflswsp ftplsrlafg vylihllfiq lflcisrerl ffshffavsl ffttivwsyl
      541 lsyllficce aptgrldklv fepqskqtek sagdaapnan gtedglpqvv aklrda
//
LOCUS       XP_077501066             729 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077501066
VERSION     XP_077501066.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644940.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..729
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=82392
     Region          75..201
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          295..698
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..729
                     /gene="LOC144111902"
                     /coded_by="XM_077644940.1:359..2548"
                     /db_xref="GeneID:144111902"
ORIGIN      
        1 mrsrkrvisl ltrlffalav viclratpcv ahieannqtd ekiveklkew igdaidrapp
       61 sfmrkllead vsskcslgll klvrgvrnle pwalrlfdas gkyptgafql srahlgafde
      121 cletvqldey gremvrgqyc nllvyatnns dlndliisam afthprvrkf tgslfeqrlp
      181 lvrlgicqld dctqdelqal lvavlpasad vsikncvtav ntaftkgqvl iisflgvlal
      241 cvmistvvdi fssqedpkrk nkgallsvlk sfsvasntrm mlhiardkss dacslrflhg
      301 irffsivwvv mghcycspsd vwsrmmnmii yvdqwqniil tagfigvdsf fflsgyllas
      361 ivwkqkrtgv vvflfavlrr lfrtmvpvff llmclqllpl iasgpdskaf fdkidedfrr
      421 qwlaillqvq nyafkkdaia pvfghfwyll vdfqfflvtl pillllknrp kvtvgvlalv
      481 slvgcliamw qvagsrktpf ivliteslst fletgyyyyf ypfyhavcyf tgcitfflvl
      541 rfttrkislp vqaaawcvvv acgmccifmk tpwyrtrdpt telgklsaaf fdgilwsvfl
      601 switlscatg rggfvntfls wspftplsrl afgvylihll fiqlllcisr ermffshffa
      661 vslffttivw syllsyllfi cceaptgrld klvfepqrkq teksdgdaap nangmedgla
      721 hvvaklrdt
//
LOCUS       XP_077501068             696 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 1-like isoform
            X1 [Amblyomma americanum].
ACCESSION   XP_077501068
VERSION     XP_077501068.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644942.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..696
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 1-like isoform X1"
                     /calculated_mol_wt=79703
     Region          <23..>106
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          23..50
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(24..25,28..30,32..33,37,40,50,52,54,58..59,62..64,
                     66..67,71,74,83,85,87,91,94,96..97,101,104)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          52..83
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          113..229
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          278..393
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          392..>447
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     Region          <464..617
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..696
                     /gene="LOC144111903"
                     /coded_by="XM_077644942.1:116..2206"
                     /db_xref="GeneID:144111903"
ORIGIN      
        1 mrlekdalia eshdglrrnt lelflscrtg dlarvkylve eqeselnvrd rwdgtplyya
       61 clcghkdvve yllsqgascv antfdgercl yaslsleird llrdrkvits ntmrrdtyde
      121 flrrcledsq hcdvsfsvqg eiipahrcvl aarcdffrrs lldkwagrql ipvthhavdg
      181 avfriitqyl ytgrlemqak hfesflelas rcqlisliad vkealaevln qgsqrsgvsa
      241 esetillept hyreqlqrdf stlpvelasg vtaggaafpa eehnhadici nvdgthflch
      301 kvflcnrsey fraliedhft easrlgsdqq qlpvielqqv tpnifgcvlr hvysnsddrv
      361 natnvwdvlc aadvyllpdl krqcgaqiar lldvesicgt lrasrlfrlp rlenqciefm
      421 akhlaeviel pefheaired akevklrsfl kthfyyyfyp fyhavcyftg citffavasf
      481 krskmpkvlq atawciavac gmccifmktv wyrtrdptte fgklsaaffd rilwsiflaw
      541 itlscatgrg gflnkflsws aftplsrlaf gvyiihlpfi hlclhisrer iffshfsvvs
      601 fffsmlvwsy llsyllfifc eaptgrldkl ifdpqrrkte grekdeapnd nsvqdklpda
      661 vvtfrdrkld iwqtsseknt sngilpgncs rtschl
//
LOCUS       XP_077501069             638 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 1-like isoform
            X2 [Amblyomma americanum].
ACCESSION   XP_077501069
VERSION     XP_077501069.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644943.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..638
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 1-like isoform X2"
                     /calculated_mol_wt=72964
     Region          <23..>106
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          23..50
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(24..25,28..30,32..33,37,40,50,52,54,58..59,62..64,
                     66..67,71,74,83,85,87,91,94,96..97,101,104)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          52..83
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          113..229
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          278..335
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          334..>389
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     Region          <406..559
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..638
                     /gene="LOC144111903"
                     /coded_by="XM_077644943.1:116..2032"
                     /db_xref="GeneID:144111903"
ORIGIN      
        1 mrlekdalia eshdglrrnt lelflscrtg dlarvkylve eqeselnvrd rwdgtplyya
       61 clcghkdvve yllsqgascv antfdgercl yaslsleird llrdrkvits ntmrrdtyde
      121 flrrcledsq hcdvsfsvqg eiipahrcvl aarcdffrrs lldkwagrql ipvthhavdg
      181 avfriitqyl ytgrlemqak hfesflelas rcqlisliad vkealaevln qgsqrsgvsa
      241 esetillept hyreqlqrdf stlpvelasg vtaggaafpa eehnhadici nvdgthflch
      301 kvnatnvwdv lcaadvyllp dlkrqcgaqi arlldvesic gtlrasrlfr lprlenqcie
      361 fmakhlaevi elpefheair edakevklrs flkthfyyyf ypfyhavcyf tgcitffava
      421 sfkrskmpkv lqatawciav acgmccifmk tvwyrtrdpt tefgklsaaf fdrilwsifl
      481 awitlscatg rggflnkfls wsaftplsrl afgvyiihlp fihlclhisr eriffshfsv
      541 vsfffsmlvw syllsyllfi fceaptgrld klifdpqrrk tegrekdeap ndnsvqdklp
      601 davvtfrdrk ldiwqtssek ntsngilpgn csrtschl
//
LOCUS       XP_077501070             584 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 1-like isoform
            X3 [Amblyomma americanum].
ACCESSION   XP_077501070
VERSION     XP_077501070.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644944.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526484) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..584
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 1-like isoform X3"
                     /calculated_mol_wt=66904
     Region          1..117
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          166..281
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          280..>335
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     Region          <352..505
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..584
                     /gene="LOC144111903"
                     /coded_by="XM_077644944.1:333..2087"
                     /db_xref="GeneID:144111903"
ORIGIN      
        1 mrrdtydefl rrcledsqhc dvsfsvqgei ipahrcvlaa rcdffrrsll dkwagrqlip
       61 vthhavdgav friitqylyt grlemqakhf esflelasrc qlisliadvk ealaevlnqg
      121 sqrsgvsaes etillepthy reqlqrdfst lpvelasgvt aggaafpaee hnhadicinv
      181 dgthflchkv flcnrseyfr aliedhftea srlgsdqqql pvielqqvtp nifgcvlrhv
      241 ysnsddrvna tnvwdvlcaa dvyllpdlkr qcgaqiarll dvesicgtlr asrlfrlprl
      301 enqciefmak hlaevielpe fheairedak evklrsflkt hfyyyfypfy havcyftgci
      361 tffavasfkr skmpkvlqat awciavacgm ccifmktvwy rtrdpttefg klsaaffdri
      421 lwsiflawit lscatgrggf lnkflswsaf tplsrlafgv yiihlpfihl clhisrerif
      481 fshfsvvsff fsmlvwsyll syllfifcea ptgrldklif dpqrrktegr ekdeapndns
      541 vqdklpdavv tfrdrkldiw qtssekntsn gilpgncsrt schl
//
LOCUS       XP_077501072             150 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111906 [Amblyomma americanum].
ACCESSION   XP_077501072
VERSION     XP_077501072.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644946.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..150
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..150
                     /product="uncharacterized protein LOC144111906"
                     /calculated_mol_wt=17326
     Region          13..100
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..150
                     /gene="LOC144111906"
                     /coded_by="XM_077644946.1:245..697"
                     /db_xref="GeneID:144111906"
ORIGIN      
        1 maaavgprav straswtdre vecflalise kkvsealdsr rqrnqdvfkd lqaemanlgy
       61 hwtwqqlrnc wknlkkrfta erleqersga apsawkwydh msallshrpm vqareygvds
      121 qdvppdygdn sqlgkytpkc vftlvyfyvm
//
LOCUS       XP_077501074             398 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111907 [Amblyomma americanum].
ACCESSION   XP_077501074
VERSION     XP_077501074.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644948.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..398
                     /product="uncharacterized protein LOC144111907"
                     /calculated_mol_wt=44643
     Region          <163..>248
                     /region_name="MscS_porin"
                     /note="Mechanosensitive ion channel porin domain;
                     pfam12795"
                     /db_xref="CDD:432790"
     CDS             1..398
                     /gene="LOC144111907"
                     /coded_by="XM_077644948.1:300..1496"
                     /db_xref="GeneID:144111907"
ORIGIN      
        1 mstdvplfvq vltgffsial gravsadsgf nrhpasadaf rlpcaggvhf shcellswhv
       61 dttfcapsss tatsstlpar ilpikgtpap sissghgnpv vtmstdvplf vqvggrkycc
      121 rsnclflivl pcprtllvcf sdcisivgll lklsgdveen pgpdtmemiq qviasqteil
      181 sklseiqenq tssearildm qsrlsaieqk lqtfdesrdr lskletfaer ceiemttvsr
      241 kldelenrsr rnnlivrgvk eeeseseedl lqkvnndifd kilkqrvdti erihrlgkre
      301 pgrdrpiiik ltdfrdkmki msncfnlkgt qfsvnedfsk rvveirknlw nscaderkng
      361 vkaklifdkl kvkntlyawn eetkqrfkcq vatstsne
//
LOCUS       XP_077501075             296 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111908 [Amblyomma americanum].
ACCESSION   XP_077501075
VERSION     XP_077501075.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644949.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..296
                     /product="uncharacterized protein LOC144111908"
                     /calculated_mol_wt=34122
     CDS             1..296
                     /gene="LOC144111908"
                     /coded_by="XM_077644949.1:150..1040"
                     /db_xref="GeneID:144111908"
ORIGIN      
        1 mstdvplfvq vggrkyccrs nclflivlpc prtllvcfsd cisivglllk lsgdveenpg
       61 pdtmemiqqv iasqteilsk lseiqenqts searildmqs rlsaieqklq tfdesrdrls
      121 kletfaerce iemttvsrkl delenrsrrn nlivrgvkee eseseedllq kvnndifdki
      181 lkqrvdtier ihrlgkrepg rdrpiiiklt dfrdkmkims ncfnlkgtqf svnedfskrv
      241 veirknlwns caderkngvk aklifdklkv kntlyawnee tkqrfkcqva tstsne
//
LOCUS       XP_077501076             327 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111911 [Amblyomma americanum].
ACCESSION   XP_077501076
VERSION     XP_077501076.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644950.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..327
                     /product="uncharacterized protein LOC144111911"
                     /calculated_mol_wt=35033
     CDS             1..327
                     /gene="LOC144111911"
                     /coded_by="XM_077644950.1:1..984"
                     /db_xref="GeneID:144111911"
ORIGIN      
        1 mlhlwshegv argpstnqwe eapavaepve gsrvereede sssafedlha pllraggslr
       61 qrslaarlrr lsrlslsags sadcsapekh arnceaavar pptkgilrrq kvhccsssgh
      121 rgttsfgtes sdrspgrfck ralgrvfgla svvdeavpre pkivsiaepf yksprrggdg
      181 wrhpcatnrp rkpplvfggt fpidepvglr gdapfrsvat fpvdafccaa pnlceghaqh
      241 qpadsslrts rqagaaavts tsesepesrv lsfskvpqag plrlhkhgql ppgssgetkt
      301 vafltqpkwk gssflhflsg dskvhks
//
LOCUS       XP_077501077            2014 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501077
VERSION     XP_077501077.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644951.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2014
                     /product="uncharacterized protein LOC144111913 isoform X1"
                     /calculated_mol_wt=218664
     Region          <357..679
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          642..>876
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1113..>1187
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1161..1525
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1283..1284,1286..1287,1289..1293,1295,1297,1307,
                     1309,1311..1312,1314..1315,1319,1325,1332,1341,1346,1349,
                     1377..1378)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1450,1472..1473,1475..1476,1479..1480,1483,
                     1512..1513,1516,1520)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..2014
                     /gene="LOC144111913"
                     /coded_by="XM_077644951.1:93..6137"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqtccc lamqegwpsa vtgaangsta gsavhcggpa qgdnywnwaa hqepgaggdt
      121 aasapmqswn swgaaqevwt graqppdpat vggtneaava pvvndgtslp pqlgytsppe
      181 ggqqqygsgg vvaegalqeg waaphwsgdt nqwpsnleqq qfvtaqaqwq wqpadassav
      241 gqwqgygsql dpaaagpyes tslagyapld gslqqqqqqp gqdqlaassg envagdmhlv
      301 ayssgpaavd gqggsegtqh leatlgdngm vsmffqdgga ddgqaesreg gptthdqhae
      361 epgppshqqq taatgiaqda isqvapaass desvnqevle pvmrpepqdv lplqaegspp
      421 ageeavlvmp egqcpapakk avirarqgga fkppalarqg ssstetdylr viyqllwylw
      481 halmqqnaiq lhlppenlel lppdevgrhh aslrhrqgva spaavgaglt lldapemqpc
      541 vtlapaappi phdrpasras tgpqvapeat gceeeesvpd ghcqtpvtrm vapplmrqsp
      601 lgreegtgrs taapapslrv atvlapspll adttlperte psgstdgdqp qpvvtaarag
      661 edkeglpvpr pkatstptgh csahheppnv svvlahgkhd skdlgdkgse shtrqeepre
      721 gkqddrnsrd frdsrdfres rdpvhsrdyr dsrdagqarg rgsdgesldw kgsqdnfehd
      781 ssrvgrgdlr ddqdrkrdry yeraddrrea skedrdeyrk agrkigtted rkrkddyydd
      841 rradqledrr edrregfrdd yrktdrrgdr yhdrrdqwkg qesddewker rsdrrsdrys
      901 rhdyryddrh sdrysdrgrp ssrtsqdayl deydqrvgdr rvrdnhnrps srhsesdsss
      961 pdsrrgygtg khrkyreadr apgvyydhqk sygtaggydy nayrrdyyny yhygyaprsg
     1021 ygygyydely rtnpaykqqv dayyarlgys paqlmerlsv hsgrssangd srrdesfasn
     1081 teddcdpsil dnestrldhv pqpeevkkfs rphplarfcg angllklvpt lsggnlpppi
     1141 eihslkhlfq kdpqfhelkq fpgplvrsqt hkndviqfct hkiqsfaedp nlpdrsshil
     1201 lwellvlmlr qngfvsgsdi aelllrghni lepvvsasgl arrggeggem tspegaddgs
     1261 spspdegivv sdraqpgatg ssqtlakfre fllfghkkda lewavkcglw ghalslaskm
     1321 dartyasimt rftsalatnd plqtlyqhls grqpaavtci adekwgdwrp hlamilsnps
     1381 srpevdarsi ttlgdvlasr gclsaahfcy lmaqvefgsy sckssklvll gsshqtllfe
     1441 efasneaiqc teiyeysqsl anagymlphl qaykflhatr laeygfaqea lhycevlaea
     1501 mgrqcgsaql aaqtyelasr lkyhdphfsq gpgeleelge ppwlaafaql vtqllqqhaa
     1561 aappqigaly gqvsscggna vaevsggdsy ldqpgftsst gfsmhdsgms ileqsgnygn
     1621 syldpsacgd saqavspsad qwtalahtap dsrsqhlppt svpsfaatgt svesgwtavq
     1681 psaeysfvst sppvesmvng gqtdgtapss ppvgsqtttf dyykastysq aqhpphhqts
     1741 rerlssvsss tdahdrrdsv dsaalasgrs rlpsgpqspl ttdqsasese rtkpaatnkg
     1801 ktqvagkswl ggifgkllpk gpnqmilpdd kdpdivwdeh kkcwvdknaa pgdadiqsaa
     1861 pptdssfggk ptppsgkpge nrfqmakqrs lrkhyvdvln pgsskasgds pavpgvppvp
     1921 eqpgtqsppq flvpqavddh cdsgydfvsp apmpdhsapa ppppppetvs yqqpsgkvvs
     1981 tpmmfdpadf ssravdsshk maggnlarrr aypt
//
LOCUS       XP_077501078            2013 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501078
VERSION     XP_077501078.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644952.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2013
                     /product="uncharacterized protein LOC144111913 isoform X2"
                     /calculated_mol_wt=218535
     Region          <357..679
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          642..>876
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1113..>1187
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1161..1525
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1283..1284,1286..1287,1289..1293,1295,1297,1307,
                     1309,1311..1312,1314..1315,1319,1325,1332,1341,1346,1349,
                     1377..1378)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1450,1472..1473,1475..1476,1479..1480,1483,
                     1512..1513,1516,1520)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..2013
                     /gene="LOC144111913"
                     /coded_by="XM_077644952.1:93..6134"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqtccc lamqegwpsa vtgaangsta gsavhcggpa qgdnywnwaa hqepgaggdt
      121 aasapmqswn swgaaqevwt graqppdpat vggtneaava pvvndgtslp pqlgytsppe
      181 ggqqqygsgg vvaegalqeg waaphwsgdt nqwpsnleqq qfvtaqaqwq wqpadassav
      241 gqwqgygsql dpaaagpyes tslagyapld gslqqqqqqp gqdqlaassg envagdmhlv
      301 ayssgpaavd gqggsegtqh leatlgdngm vsmffqdgga ddgqaesreg gptthdqhae
      361 epgppshqqq taatgiaqda isqvapaass desvnqevle pvmrpepqdv lplqaegspp
      421 ageeavlvmp egqcpapakk avirarqgga fkppalarqg ssstetdylr viyqllwylw
      481 halmqqnaiq lhlppenlel lppdevgrhh aslrhrqgva spaavgaglt lldapemqpc
      541 vtlapaappi phdrpasras tgpqvapeat gceeeesvpd ghcqtpvtrm vapplmrqsp
      601 lgreegtgrs taapapslrv atvlapspll adttlperte psgstdgdqp qpvvtaarag
      661 edkeglpvpr pkatstptgh csahheppnv svvlahgkhd skdlgdkgse shtrqeepre
      721 gkqddrnsrd frdsrdfres rdpvhsrdyr dsrdagqarg rgsdgesldw kgsqdnfehd
      781 ssrvgrgdlr ddqdrkrdry yeraddrrea skedrdeyrk agrkigtted rkrkddyydd
      841 rradqledrr edrregfrdd yrktdrrgdr yhdrrdqwkg qesddewker rsdrrsdrys
      901 rhdyryddrh sdrysdrgrp ssrtsqdayl deydqrvgdr rvrdnhnrps srhsesdsss
      961 pdsrrgygtg khrkyreadr apgvyydhqk sygtaggydy nayrrdyyny yhygyaprsg
     1021 ygygyydely rtnpaykqqv dayyarlgys paqlmerlsv hsgrssangd srrdesfasn
     1081 teddcdpsil dnestrldhv pqpeevkkfs rphplarfcg angllklvpt lsggnlpppi
     1141 eihslkhlfq kdpqfhelkq fpgplvrsqt hkndviqfct hkiqsfaedp nlpdrsshil
     1201 lwellvlmlr qngfvsgsdi aelllrghni lepvvsasgl arrggeggem tspegaddgs
     1261 spspdegivv sdraqpgatg ssqtlakfre fllfghkkda lewavkcglw ghalslaskm
     1321 dartyasimt rftsalatnd plqtlyqhls grqpaavtci adekwgdwrp hlamilsnps
     1381 srpevdarsi ttlgdvlasr gclsaahfcy lmaqvefgsy sckssklvll gsshqtllfe
     1441 efasneaiqc teiyeysqsl anagymlphl qaykflhatr laeygfaqea lhycevlaea
     1501 mgrqcgsaql aaqtyelasr lkyhdphfsq gpgeleelge ppwlaafaql vtqllqqhaa
     1561 aappqigaly gqvsscggna vaevsggdsy ldqpgftsst gfsmhdsgms ileqsgnygn
     1621 syldpsacgd saqavspsad qwtalahtap dsrsqhlppt svpsfaatgt svesgwtavq
     1681 psaeysfvst sppvesmvng gqtdgtapss ppvgsqtttf dyykastysa qhpphhqtsr
     1741 erlssvssst dahdrrdsvd saalasgrsr lpsgpqsplt tdqsaseser tkpaatnkgk
     1801 tqvagkswlg gifgkllpkg pnqmilpddk dpdivwdehk kcwvdknaap gdadiqsaap
     1861 ptdssfggkp tppsgkpgen rfqmakqrsl rkhyvdvlnp gsskasgdsp avpgvppvpe
     1921 qpgtqsppqf lvpqavddhc dsgydfvspa pmpdhsapap pppppetvsy qqpsgkvvst
     1981 pmmfdpadfs sravdsshkm aggnlarrra ypt
//
LOCUS       XP_077501079            2013 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501079
VERSION     XP_077501079.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644953.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2013
                     /product="uncharacterized protein LOC144111913 isoform X3"
                     /calculated_mol_wt=218535
     Region          <356..678
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          641..>875
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1112..>1186
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1160..1524
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1282..1283,1285..1286,1288..1292,1294,1296,1306,
                     1308,1310..1311,1313..1314,1318,1324,1331,1340,1345,1348,
                     1376..1377)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1449,1471..1472,1474..1475,1478..1479,1482,
                     1511..1512,1515,1519)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..2013
                     /gene="LOC144111913"
                     /coded_by="XM_077644953.1:93..6134"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqtccc lamqegwpsa vtgaangsta gsavhcggpa qgdnywnwaa hqepgaggdt
      121 aasapmqswn swgaaqevwt graqppdpat vggtneaava pvvndgtslp pqlgytsppe
      181 ggqqqygsgg vvaegalqeg waaphwsgdt nqwpsnleqq qfvtaqaqww qpadassavg
      241 qwqgygsqld paaagpyest slagyapldg slqqqqqqpg qdqlaassge nvagdmhlva
      301 yssgpaavdg qggsegtqhl eatlgdngmv smffqdggad dgqaesregg ptthdqhaee
      361 pgppshqqqt aatgiaqdai sqvapaassd esvnqevlep vmrpepqdvl plqaegsppa
      421 geeavlvmpe gqcpapakka virarqggaf kppalarqgs sstetdylrv iyqllwylwh
      481 almqqnaiql hlppenlell ppdevgrhha slrhrqgvas paavgagltl ldapemqpcv
      541 tlapaappip hdrpasrast gpqvapeatg ceeeesvpdg hcqtpvtrmv applmrqspl
      601 greegtgrst aapapslrva tvlapsplla dttlpertep sgstdgdqpq pvvtaarage
      661 dkeglpvprp katstptghc sahheppnvs vvlahgkhds kdlgdkgses htrqeepreg
      721 kqddrnsrdf rdsrdfresr dpvhsrdyrd srdagqargr gsdgesldwk gsqdnfehds
      781 srvgrgdlrd dqdrkrdryy eraddrreas kedrdeyrka grkigttedr krkddyyddr
      841 radqledrre drregfrddy rktdrrgdry hdrrdqwkgq esddewkerr sdrrsdrysr
      901 hdyryddrhs drysdrgrps srtsqdayld eydqrvgdrr vrdnhnrpss rhsesdsssp
      961 dsrrgygtgk hrkyreadra pgvyydhqks ygtaggydyn ayrrdyynyy hygyaprsgy
     1021 gygyydelyr tnpaykqqvd ayyarlgysp aqlmerlsvh sgrssangds rrdesfasnt
     1081 eddcdpsild nestrldhvp qpeevkkfsr phplarfcga ngllklvptl sggnlpppie
     1141 ihslkhlfqk dpqfhelkqf pgplvrsqth kndviqfcth kiqsfaedpn lpdrsshill
     1201 wellvlmlrq ngfvsgsdia elllrghnil epvvsasgla rrggeggemt spegaddgss
     1261 pspdegivvs draqpgatgs sqtlakfref llfghkkdal ewavkcglwg halslaskmd
     1321 artyasimtr ftsalatndp lqtlyqhlsg rqpaavtcia dekwgdwrph lamilsnpss
     1381 rpevdarsit tlgdvlasrg clsaahfcyl maqvefgsys ckssklvllg sshqtllfee
     1441 fasneaiqct eiyeysqsla nagymlphlq aykflhatrl aeygfaqeal hycevlaeam
     1501 grqcgsaqla aqtyelasrl kyhdphfsqg pgeleelgep pwlaafaqlv tqllqqhaaa
     1561 appqigalyg qvsscggnav aevsggdsyl dqpgftsstg fsmhdsgmsi leqsgnygns
     1621 yldpsacgds aqavspsadq wtalahtapd srsqhlppts vpsfaatgts vesgwtavqp
     1681 saeysfvsts ppvesmvngg qtdgtapssp pvgsqtttfd yykastysqa qhpphhqtsr
     1741 erlssvssst dahdrrdsvd saalasgrsr lpsgpqsplt tdqsaseser tkpaatnkgk
     1801 tqvagkswlg gifgkllpkg pnqmilpddk dpdivwdehk kcwvdknaap gdadiqsaap
     1861 ptdssfggkp tppsgkpgen rfqmakqrsl rkhyvdvlnp gsskasgdsp avpgvppvpe
     1921 qpgtqsppqf lvpqavddhc dsgydfvspa pmpdhsapap pppppetvsy qqpsgkvvst
     1981 pmmfdpadfs sravdsshkm aggnlarrra ypt
//
LOCUS       XP_077501080            2013 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077501080
VERSION     XP_077501080.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644954.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2013
                     /product="uncharacterized protein LOC144111913 isoform X4"
                     /calculated_mol_wt=218535
     Region          <357..679
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          642..>876
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1113..>1187
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1161..1525
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1283..1284,1286..1287,1289..1293,1295,1297,1307,
                     1309,1311..1312,1314..1315,1319,1325,1332,1341,1346,1349,
                     1377..1378)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1450,1472..1473,1475..1476,1479..1480,1483,
                     1512..1513,1516,1520)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..2013
                     /gene="LOC144111913"
                     /coded_by="XM_077644954.1:93..6134"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqtccc lamqegwpsa vtgaangsta gsavhcggpa qgdnywnwaa hqepgaggdt
      121 aasapmqswn swgaaqevwt graqppdpat vggtneaava pvvndgtslp pqlgytsppe
      181 ggqqqygsgg vvaegalqeg waaphwsgdt nqwpsnleqq qfvtaqaqwq wqpadassav
      241 gqwqgygsql dpaaagpyes tslagyapld gslqqqqqqp gqdqlaassg envagdmhlv
      301 ayssgpaavd gqggsegtqh leatlgdngm vsmffqdgga ddgqaesreg gptthdqhae
      361 epgppshqqq taatgiaqda isqvapaass desvnqevle pvmrpepqdv lplqaegspp
      421 ageeavlvmp egqcpapakk avirarqgga fkppalarqg ssstetdylr viyqllwylw
      481 halmqqnaiq lhlppenlel lppdevgrhh aslrhrqgva spaavgaglt lldapemqpc
      541 vtlapaappi phdrpasras tgpqvapeat gceeeesvpd ghcqtpvtrm vapplmrqsp
      601 lgreegtgrs taapapslrv atvlapspll adttlperte psgstdgdqp qpvvtaarag
      661 edkeglpvpr pkatstptgh csahheppnv svvlahgkhd skdlgdkgse shtrqeepre
      721 gkqddrnsrd frdsrdfres rdpvhsrdyr dsrdagqarg rgsdgesldw kgsqdnfehd
      781 ssrvgrgdlr ddqdrkrdry yeraddrrea skedrdeyrk agrkigtted rkrkddyydd
      841 rradqledrr edrregfrdd yrktdrrgdr yhdrrdqwkg qesddewker rsdrrsdrys
      901 rhdyryddrh sdrysdrgrp ssrtsqdayl deydqrvgdr rvrdnhnrps srhsesdsss
      961 pdsrrgygtg khrkyreadr apgvyydhqk sygtaggydy nayrrdyyny yhygyaprsg
     1021 ygygyydely rtnpaykqqv dayyarlgys paqlmerlsv hsgrssangd srrdesfasn
     1081 teddcdpsil dnestrldhv pqpeevkkfs rphplarfcg angllklvpt lsggnlpppi
     1141 eihslkhlfq kdpqfhelkq fpgplvrsqt hkndviqfct hkiqsfaedp nlpdrsshil
     1201 lwellvlmlr qngfvsgsdi aelllrghni lepvvsasgl arrggeggem tspegaddgs
     1261 spspdegivv sdraqpgatg ssqtlakfre fllfghkkda lewavkcglw ghalslaskm
     1321 dartyasimt rftsalatnd plqtlyqhls grqpaavtci adekwgdwrp hlamilsnps
     1381 srpevdarsi ttlgdvlasr gclsaahfcy lmaqvefgsy sckssklvll gsshqtllfe
     1441 efasneaiqc teiyeysqsl anagymlphl qaykflhatr laeygfaqea lhycevlaea
     1501 mgrqcgsaql aaqtyelasr lkyhdphfsq gpgeleelge ppwlaafaql vtqllqqhaa
     1561 aappqigaly gqvsscggna vaevsggdsy ldqpgftsst gfsmhdsgms ileqsgnygn
     1621 syldpsacgd saqavspsad qwtalahtap dsrsqhlppt svpsfaatgt svesgwtavq
     1681 psaeysfvst sppvesmvng gqtdgtapss ppvgsqtttf dyykastysq aqhpphhqts
     1741 rerlssvsss tdahdrrdsv dsaalasgrs rlpsgpqspl ttdqsasese rtkpaatnkg
     1801 ktqvagkswl ggifgkllpk gpnqmilpdd kdpdivwdeh kkcwvdknaa pgdadiqsaa
     1861 pptdssfggk ptppsgkpge nrfqmakqrs lrkhyvdvln pgsskasgds pavpgvppvp
     1921 eqpgtqsppq flvpqavddh cdsgydfvsp apmpdhsapa ppppppetvs yqpsgkvvst
     1981 pmmfdpadfs sravdsshkm aggnlarrra ypt
//
LOCUS       XP_077501081            2012 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077501081
VERSION     XP_077501081.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644955.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2012
                     /product="uncharacterized protein LOC144111913 isoform X5"
                     /calculated_mol_wt=218407
     Region          <357..679
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          642..>876
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1113..>1187
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1161..1525
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1283..1284,1286..1287,1289..1293,1295,1297,1307,
                     1309,1311..1312,1314..1315,1319,1325,1332,1341,1346,1349,
                     1377..1378)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1450,1472..1473,1475..1476,1479..1480,1483,
                     1512..1513,1516,1520)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..2012
                     /gene="LOC144111913"
                     /coded_by="XM_077644955.1:93..6131"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqtccc lamqegwpsa vtgaangsta gsavhcggpa qgdnywnwaa hqepgaggdt
      121 aasapmqswn swgaaqevwt graqppdpat vggtneaava pvvndgtslp pqlgytsppe
      181 ggqqqygsgg vvaegalqeg waaphwsgdt nqwpsnleqq qfvtaqaqwq wqpadassav
      241 gqwqgygsql dpaaagpyes tslagyapld gslqqqqqqp gqdqlaassg envagdmhlv
      301 ayssgpaavd gqggsegtqh leatlgdngm vsmffqdgga ddgqaesreg gptthdqhae
      361 epgppshqqq taatgiaqda isqvapaass desvnqevle pvmrpepqdv lplqaegspp
      421 ageeavlvmp egqcpapakk avirarqgga fkppalarqg ssstetdylr viyqllwylw
      481 halmqqnaiq lhlppenlel lppdevgrhh aslrhrqgva spaavgaglt lldapemqpc
      541 vtlapaappi phdrpasras tgpqvapeat gceeeesvpd ghcqtpvtrm vapplmrqsp
      601 lgreegtgrs taapapslrv atvlapspll adttlperte psgstdgdqp qpvvtaarag
      661 edkeglpvpr pkatstptgh csahheppnv svvlahgkhd skdlgdkgse shtrqeepre
      721 gkqddrnsrd frdsrdfres rdpvhsrdyr dsrdagqarg rgsdgesldw kgsqdnfehd
      781 ssrvgrgdlr ddqdrkrdry yeraddrrea skedrdeyrk agrkigtted rkrkddyydd
      841 rradqledrr edrregfrdd yrktdrrgdr yhdrrdqwkg qesddewker rsdrrsdrys
      901 rhdyryddrh sdrysdrgrp ssrtsqdayl deydqrvgdr rvrdnhnrps srhsesdsss
      961 pdsrrgygtg khrkyreadr apgvyydhqk sygtaggydy nayrrdyyny yhygyaprsg
     1021 ygygyydely rtnpaykqqv dayyarlgys paqlmerlsv hsgrssangd srrdesfasn
     1081 teddcdpsil dnestrldhv pqpeevkkfs rphplarfcg angllklvpt lsggnlpppi
     1141 eihslkhlfq kdpqfhelkq fpgplvrsqt hkndviqfct hkiqsfaedp nlpdrsshil
     1201 lwellvlmlr qngfvsgsdi aelllrghni lepvvsasgl arrggeggem tspegaddgs
     1261 spspdegivv sdraqpgatg ssqtlakfre fllfghkkda lewavkcglw ghalslaskm
     1321 dartyasimt rftsalatnd plqtlyqhls grqpaavtci adekwgdwrp hlamilsnps
     1381 srpevdarsi ttlgdvlasr gclsaahfcy lmaqvefgsy sckssklvll gsshqtllfe
     1441 efasneaiqc teiyeysqsl anagymlphl qaykflhatr laeygfaqea lhycevlaea
     1501 mgrqcgsaql aaqtyelasr lkyhdphfsq gpgeleelge ppwlaafaql vtqllqqhaa
     1561 aappqigaly gqvsscggna vaevsggdsy ldqpgftsst gfsmhdsgms ileqsgnygn
     1621 syldpsacgd saqavspsad qwtalahtap dsrsqhlppt svpsfaatgt svesgwtavq
     1681 psaeysfvst sppvesmvng gqtdgtapss ppvgsqtttf dyykastysa qhpphhqtsr
     1741 erlssvssst dahdrrdsvd saalasgrsr lpsgpqsplt tdqsaseser tkpaatnkgk
     1801 tqvagkswlg gifgkllpkg pnqmilpddk dpdivwdehk kcwvdknaap gdadiqsaap
     1861 ptdssfggkp tppsgkpgen rfqmakqrsl rkhyvdvlnp gsskasgdsp avpgvppvpe
     1921 qpgtqsppqf lvpqavddhc dsgydfvspa pmpdhsapap pppppetvsy qpsgkvvstp
     1981 mmfdpadfss ravdsshkma ggnlarrray pt
//
LOCUS       XP_077501082            2011 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077501082
VERSION     XP_077501082.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644956.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2011
                     /product="uncharacterized protein LOC144111913 isoform X6"
                     /calculated_mol_wt=218048
     Region          <354..676
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          639..>873
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1110..>1184
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1158..1522
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1280..1281,1283..1284,1286..1290,1292,1294,1304,
                     1306,1308..1309,1311..1312,1316,1322,1329,1338,1343,1346,
                     1374..1375)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1447,1469..1470,1472..1473,1476..1477,1480,
                     1509..1510,1513,1517)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..2011
                     /gene="LOC144111913"
                     /coded_by="XM_077644956.1:21..6056"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 mdcwpvgatg kaaeaaqpak tlpqpvgevm pqwdgstsfq ptqypaalld pqqqqrqqqs
       61 nlqtccclam qegwpsavtg aangstagsa vhcggpaqgd nywnwaahqe pgaggdtaas
      121 apmqswnswg aaqevwtgra qppdpatvgg tneaavapvv ndgtslppql gytsppeggq
      181 qqygsggvva egalqegwaa phwsgdtnqw psnleqqqfv taqaqwqwqp adassavgqw
      241 qgygsqldpa aagpyestsl agyapldgsl qqqqqqpgqd qlaassgenv agdmhlvays
      301 sgpaavdgqg gsegtqhlea tlgdngmvsm ffqdggaddg qaesreggpt thdqhaeepg
      361 ppshqqqtaa tgiaqdaisq vapaassdes vnqevlepvm rpepqdvlpl qaegsppage
      421 eavlvmpegq cpapakkavi rarqggafkp palarqgsss tetdylrviy qllwylwhal
      481 mqqnaiqlhl ppenlellpp devgrhhasl rhrqgvaspa avgagltlld apemqpcvtl
      541 apaappiphd rpasrastgp qvapeatgce eeesvpdghc qtpvtrmvap plmrqsplgr
      601 eegtgrstaa papslrvatv lapsplladt tlpertepsg stdgdqpqpv vtaaragedk
      661 eglpvprpka tstptghcsa hheppnvsvv lahgkhdskd lgdkgsesht rqeepregkq
      721 ddrnsrdfrd srdfresrdp vhsrdyrdsr dagqargrgs dgesldwkgs qdnfehdssr
      781 vgrgdlrddq drkrdryyer addrreaske drdeyrkagr kigttedrkr kddyyddrra
      841 dqledrredr regfrddyrk tdrrgdryhd rrdqwkgqes ddewkerrsd rrsdrysrhd
      901 yryddrhsdr ysdrgrpssr tsqdayldey dqrvgdrrvr dnhnrpssrh sesdssspds
      961 rrgygtgkhr kyreadrapg vyydhqksyg taggydynay rrdyynyyhy gyaprsgygy
     1021 gyydelyrtn paykqqvday yarlgyspaq lmerlsvhsg rssangdsrr desfasnted
     1081 dcdpsildne strldhvpqp eevkkfsrph plarfcgang llklvptlsg gnlpppieih
     1141 slkhlfqkdp qfhelkqfpg plvrsqthkn dviqfcthki qsfaedpnlp drsshillwe
     1201 llvlmlrqng fvsgsdiael llrghnilep vvsasglarr ggeggemtsp egaddgssps
     1261 pdegivvsdr aqpgatgssq tlakfrefll fghkkdalew avkcglwgha lslaskmdar
     1321 tyasimtrft salatndplq tlyqhlsgrq paavtciade kwgdwrphla milsnpssrp
     1381 evdarsittl gdvlasrgcl saahfcylma qvefgsysck ssklvllgss hqtllfeefa
     1441 sneaiqctei yeysqslana gymlphlqay kflhatrlae ygfaqealhy cevlaeamgr
     1501 qcgsaqlaaq tyelasrlky hdphfsqgpg eleelgeppw laafaqlvtq llqqhaaaap
     1561 pqigalygqv sscggnavae vsggdsyldq pgftsstgfs mhdsgmsile qsgnygnsyl
     1621 dpsacgdsaq avspsadqwt alahtapdsr sqhlpptsvp sfaatgtsve sgwtavqpsa
     1681 eysfvstspp vesmvnggqt dgtapssppv gsqtttfdyy kastysqaqh pphhqtsrer
     1741 lssvssstda hdrrdsvdsa alasgrsrlp sgpqsplttd qsasesertk paatnkgktq
     1801 vagkswlggi fgkllpkgpn qmilpddkdp divwdehkkc wvdknaapgd adiqsaappt
     1861 dssfggkptp psgkpgenrf qmakqrslrk hyvdvlnpgs skasgdspav pgvppvpeqp
     1921 gtqsppqflv pqavddhcds gydfvspapm pdhsapappp pppetvsyqq psgkvvstpm
     1981 mfdpadfssr avdsshkmag gnlarrrayp t
//
LOCUS       XP_077501083            2009 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X7 [Amblyomma
            americanum].
ACCESSION   XP_077501083
VERSION     XP_077501083.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644957.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2009
                     /product="uncharacterized protein LOC144111913 isoform X7"
                     /calculated_mol_wt=218113
     Region          <344..>754
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          637..>871
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1108..>1182
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1156..1520
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1278..1279,1281..1282,1284..1288,1290,1292,1302,
                     1304,1306..1307,1309..1310,1314,1320,1327,1336,1341,1344,
                     1372..1373)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1445,1467..1468,1470..1471,1474..1475,1478,
                     1507..1508,1511,1515)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..2009
                     /gene="LOC144111913"
                     /coded_by="XM_077644957.1:93..6122"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqtccc lamqegwpsa vtgaangsta gsavhcggpa qgdnywnwaa hqepgaggdt
      121 aasapmqswn swgaaqevwt graqppdpat vggtneaava pvvndgtslp pqlgytsppe
      181 ggqqqygsgg vvaegalqeg waaphwsgdt nqwpsnleqq qfvtaqaqwq wqpadassav
      241 gqwqgygsql dpaaagpyes tslagyapld gslqqqqqqp gqdqlaassg envagdmhlv
      301 ayssgpaavd gqggsegtqh leatlgdngm vsmffqdgga ddgqaesreg gptthdqhae
      361 epgppshqqq taatgiaqda isqvapaass desvnqevle pvmrpepqda egsppageea
      421 vlvmpegqcp apakkavira rqggafkppa larqgssste tdylrviyql lwylwhalmq
      481 qnaiqlhlpp enlellppde vgrhhaslrh rqgvaspaav gagltlldap emqpcvtlap
      541 aappiphdrp asrastgpqv apeatgceee esvpdghcqt pvtrmvappl mrqsplgree
      601 gtgrstaapa pslrvatvla psplladttl pertepsgst dgdqpqpvvt aaragedkeg
      661 lpvprpkats tptghcsahh eppnvsvvla hgkhdskdlg dkgseshtrq eepregkqdd
      721 rnsrdfrdsr dfresrdpvh srdyrdsrda gqargrgsdg esldwkgsqd nfehdssrvg
      781 rgdlrddqdr krdryyerad drreaskedr deyrkagrki gttedrkrkd dyyddrradq
      841 ledrredrre gfrddyrktd rrgdryhdrr dqwkgqesdd ewkerrsdrr sdrysrhdyr
      901 yddrhsdrys drgrpssrts qdayldeydq rvgdrrvrdn hnrpssrhse sdssspdsrr
      961 gygtgkhrky readrapgvy ydhqksygta ggydynayrr dyynyyhygy aprsgygygy
     1021 ydelyrtnpa ykqqvdayya rlgyspaqlm erlsvhsgrs sangdsrrde sfasnteddc
     1081 dpsildnest rldhvpqpee vkkfsrphpl arfcgangll klvptlsggn lpppieihsl
     1141 khlfqkdpqf helkqfpgpl vrsqthkndv iqfcthkiqs faedpnlpdr sshillwell
     1201 vlmlrqngfv sgsdiaelll rghnilepvv sasglarrgg eggemtspeg addgsspspd
     1261 egivvsdraq pgatgssqtl akfrefllfg hkkdalewav kcglwghals laskmdarty
     1321 asimtrftsa latndplqtl yqhlsgrqpa avtciadekw gdwrphlami lsnpssrpev
     1381 darsittlgd vlasrgclsa ahfcylmaqv efgsysckss klvllgsshq tllfeefasn
     1441 eaiqcteiye ysqslanagy mlphlqaykf lhatrlaeyg faqealhyce vlaeamgrqc
     1501 gsaqlaaqty elasrlkyhd phfsqgpgel eelgeppwla afaqlvtqll qqhaaaappq
     1561 igalygqvss cggnavaevs ggdsyldqpg ftsstgfsmh dsgmsileqs gnygnsyldp
     1621 sacgdsaqav spsadqwtal ahtapdsrsq hlpptsvpsf aatgtsvesg wtavqpsaey
     1681 sfvstsppve smvnggqtdg tapssppvgs qtttfdyyka stysqaqhpp hhqtsrerls
     1741 svssstdahd rrdsvdsaal asgrsrlpsg pqsplttdqs asesertkpa atnkgktqva
     1801 gkswlggifg kllpkgpnqm ilpddkdpdi vwdehkkcwv dknaapgdad iqsaapptds
     1861 sfggkptpps gkpgenrfqm akqrslrkhy vdvlnpgssk asgdspavpg vppvpeqpgt
     1921 qsppqflvpq avddhcdsgy dfvspapmpd hsapappppp petvsyqqps gkvvstpmmf
     1981 dpadfssrav dsshkmaggn larrraypt
//
LOCUS       XP_077501086            2006 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X8 [Amblyomma
            americanum].
ACCESSION   XP_077501086
VERSION     XP_077501086.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644960.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2006
                     /product="uncharacterized protein LOC144111913 isoform X8"
                     /calculated_mol_wt=217809
     Region          <349..671
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          634..>868
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1105..>1179
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1153..1517
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1275..1276,1278..1279,1281..1285,1287,1289,1299,
                     1301,1303..1304,1306..1307,1311,1317,1324,1333,1338,1341,
                     1369..1370)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1442,1464..1465,1467..1468,1471..1472,1475,
                     1504..1505,1508,1512)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..2006
                     /gene="LOC144111913"
                     /coded_by="XM_077644960.1:93..6113"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqegwp savtgaangs tagsavhcgg paqgdnywnw aahqepgagg dtaasapmqs
      121 wnswgaaqev wtgraqppdp atvggtneaa vapvvndgts lppqlgytsp peggqqqygs
      181 ggvvaegalq egwaaphwsg dtnqwpsnle qqqfvtaqaq wqwqpadass avgqwqgygs
      241 qldpaaagpy estslagyap ldgslqqqqq qpgqdqlaas sgenvagdmh lvayssgpaa
      301 vdgqggsegt qhleatlgdn gmvsmffqdg gaddgqaesr eggptthdqh aeepgppshq
      361 qqtaatgiaq daisqvapaa ssdesvnqev lepvmrpepq dvlplqaegs ppageeavlv
      421 mpegqcpapa kkavirarqg gafkppalar qgssstetdy lrviyqllwy lwhalmqqna
      481 iqlhlppenl ellppdevgr hhaslrhrqg vaspaavgag ltlldapemq pcvtlapaap
      541 piphdrpasr astgpqvape atgceeeesv pdghcqtpvt rmvapplmrq splgreegtg
      601 rstaapapsl rvatvlapsp lladttlper tepsgstdgd qpqpvvtaar agedkeglpv
      661 prpkatstpt ghcsahhepp nvsvvlahgk hdskdlgdkg seshtrqeep regkqddrns
      721 rdfrdsrdfr esrdpvhsrd yrdsrdagqa rgrgsdgesl dwkgsqdnfe hdssrvgrgd
      781 lrddqdrkrd ryyeraddrr easkedrdey rkagrkigtt edrkrkddyy ddrradqled
      841 rredrregfr ddyrktdrrg dryhdrrdqw kgqesddewk errsdrrsdr ysrhdyrydd
      901 rhsdrysdrg rpssrtsqda yldeydqrvg drrvrdnhnr pssrhsesds sspdsrrgyg
      961 tgkhrkyrea drapgvyydh qksygtaggy dynayrrdyy nyyhygyapr sgygygyyde
     1021 lyrtnpaykq qvdayyarlg yspaqlmerl svhsgrssan gdsrrdesfa snteddcdps
     1081 ildnestrld hvpqpeevkk fsrphplarf cgangllklv ptlsggnlpp pieihslkhl
     1141 fqkdpqfhel kqfpgplvrs qthkndviqf cthkiqsfae dpnlpdrssh illwellvlm
     1201 lrqngfvsgs diaelllrgh nilepvvsas glarrggegg emtspegadd gsspspdegi
     1261 vvsdraqpga tgssqtlakf refllfghkk dalewavkcg lwghalslas kmdartyasi
     1321 mtrftsalat ndplqtlyqh lsgrqpaavt ciadekwgdw rphlamilsn pssrpevdar
     1381 sittlgdvla srgclsaahf cylmaqvefg sysckssklv llgsshqtll feefasneai
     1441 qcteiyeysq slanagymlp hlqaykflha trlaeygfaq ealhycevla eamgrqcgsa
     1501 qlaaqtyela srlkyhdphf sqgpgeleel geppwlaafa qlvtqllqqh aaaappqiga
     1561 lygqvsscgg navaevsggd syldqpgfts stgfsmhdsg msileqsgny gnsyldpsac
     1621 gdsaqavsps adqwtalaht apdsrsqhlp ptsvpsfaat gtsvesgwta vqpsaeysfv
     1681 stsppvesmv nggqtdgtap ssppvgsqtt tfdyykasty sqaqhpphhq tsrerlssvs
     1741 sstdahdrrd svdsaalasg rsrlpsgpqs plttdqsase sertkpaatn kgktqvagks
     1801 wlggifgkll pkgpnqmilp ddkdpdivwd ehkkcwvdkn aapgdadiqs aapptdssfg
     1861 gkptppsgkp genrfqmakq rslrkhyvdv lnpgsskasg dspavpgvpp vpeqpgtqsp
     1921 pqflvpqavd dhcdsgydfv spapmpdhsa pappppppet vsyqqpsgkv vstpmmfdpa
     1981 dfssravdss hkmaggnlar rraypt
//
LOCUS       XP_077501087            2005 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X9 [Amblyomma
            americanum].
ACCESSION   XP_077501087
VERSION     XP_077501087.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644961.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2005
                     /product="uncharacterized protein LOC144111913 isoform X9"
                     /calculated_mol_wt=217681
     Region          <349..671
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          634..>868
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1105..>1179
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1153..1517
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1275..1276,1278..1279,1281..1285,1287,1289,1299,
                     1301,1303..1304,1306..1307,1311,1317,1324,1333,1338,1341,
                     1369..1370)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1442,1464..1465,1467..1468,1471..1472,1475,
                     1504..1505,1508,1512)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..2005
                     /gene="LOC144111913"
                     /coded_by="XM_077644961.1:93..6110"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqegwp savtgaangs tagsavhcgg paqgdnywnw aahqepgagg dtaasapmqs
      121 wnswgaaqev wtgraqppdp atvggtneaa vapvvndgts lppqlgytsp peggqqqygs
      181 ggvvaegalq egwaaphwsg dtnqwpsnle qqqfvtaqaq wqwqpadass avgqwqgygs
      241 qldpaaagpy estslagyap ldgslqqqqq qpgqdqlaas sgenvagdmh lvayssgpaa
      301 vdgqggsegt qhleatlgdn gmvsmffqdg gaddgqaesr eggptthdqh aeepgppshq
      361 qqtaatgiaq daisqvapaa ssdesvnqev lepvmrpepq dvlplqaegs ppageeavlv
      421 mpegqcpapa kkavirarqg gafkppalar qgssstetdy lrviyqllwy lwhalmqqna
      481 iqlhlppenl ellppdevgr hhaslrhrqg vaspaavgag ltlldapemq pcvtlapaap
      541 piphdrpasr astgpqvape atgceeeesv pdghcqtpvt rmvapplmrq splgreegtg
      601 rstaapapsl rvatvlapsp lladttlper tepsgstdgd qpqpvvtaar agedkeglpv
      661 prpkatstpt ghcsahhepp nvsvvlahgk hdskdlgdkg seshtrqeep regkqddrns
      721 rdfrdsrdfr esrdpvhsrd yrdsrdagqa rgrgsdgesl dwkgsqdnfe hdssrvgrgd
      781 lrddqdrkrd ryyeraddrr easkedrdey rkagrkigtt edrkrkddyy ddrradqled
      841 rredrregfr ddyrktdrrg dryhdrrdqw kgqesddewk errsdrrsdr ysrhdyrydd
      901 rhsdrysdrg rpssrtsqda yldeydqrvg drrvrdnhnr pssrhsesds sspdsrrgyg
      961 tgkhrkyrea drapgvyydh qksygtaggy dynayrrdyy nyyhygyapr sgygygyyde
     1021 lyrtnpaykq qvdayyarlg yspaqlmerl svhsgrssan gdsrrdesfa snteddcdps
     1081 ildnestrld hvpqpeevkk fsrphplarf cgangllklv ptlsggnlpp pieihslkhl
     1141 fqkdpqfhel kqfpgplvrs qthkndviqf cthkiqsfae dpnlpdrssh illwellvlm
     1201 lrqngfvsgs diaelllrgh nilepvvsas glarrggegg emtspegadd gsspspdegi
     1261 vvsdraqpga tgssqtlakf refllfghkk dalewavkcg lwghalslas kmdartyasi
     1321 mtrftsalat ndplqtlyqh lsgrqpaavt ciadekwgdw rphlamilsn pssrpevdar
     1381 sittlgdvla srgclsaahf cylmaqvefg sysckssklv llgsshqtll feefasneai
     1441 qcteiyeysq slanagymlp hlqaykflha trlaeygfaq ealhycevla eamgrqcgsa
     1501 qlaaqtyela srlkyhdphf sqgpgeleel geppwlaafa qlvtqllqqh aaaappqiga
     1561 lygqvsscgg navaevsggd syldqpgfts stgfsmhdsg msileqsgny gnsyldpsac
     1621 gdsaqavsps adqwtalaht apdsrsqhlp ptsvpsfaat gtsvesgwta vqpsaeysfv
     1681 stsppvesmv nggqtdgtap ssppvgsqtt tfdyykasty saqhpphhqt srerlssvss
     1741 stdahdrrds vdsaalasgr srlpsgpqsp lttdqsases ertkpaatnk gktqvagksw
     1801 lggifgkllp kgpnqmilpd dkdpdivwde hkkcwvdkna apgdadiqsa apptdssfgg
     1861 kptppsgkpg enrfqmakqr slrkhyvdvl npgsskasgd spavpgvppv peqpgtqspp
     1921 qflvpqavdd hcdsgydfvs papmpdhsap appppppetv syqqpsgkvv stpmmfdpad
     1981 fssravdssh kmaggnlarr raypt
//
LOCUS       XP_077501088            2005 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X9 [Amblyomma
            americanum].
ACCESSION   XP_077501088
VERSION     XP_077501088.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644962.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2005
                     /product="uncharacterized protein LOC144111913 isoform X9"
                     /calculated_mol_wt=217681
     Region          <349..671
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          634..>868
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1105..>1179
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1153..1517
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1275..1276,1278..1279,1281..1285,1287,1289,1299,
                     1301,1303..1304,1306..1307,1311,1317,1324,1333,1338,1341,
                     1369..1370)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1442,1464..1465,1467..1468,1471..1472,1475,
                     1504..1505,1508,1512)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..2005
                     /gene="LOC144111913"
                     /coded_by="XM_077644962.1:158..6175"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqegwp savtgaangs tagsavhcgg paqgdnywnw aahqepgagg dtaasapmqs
      121 wnswgaaqev wtgraqppdp atvggtneaa vapvvndgts lppqlgytsp peggqqqygs
      181 ggvvaegalq egwaaphwsg dtnqwpsnle qqqfvtaqaq wqwqpadass avgqwqgygs
      241 qldpaaagpy estslagyap ldgslqqqqq qpgqdqlaas sgenvagdmh lvayssgpaa
      301 vdgqggsegt qhleatlgdn gmvsmffqdg gaddgqaesr eggptthdqh aeepgppshq
      361 qqtaatgiaq daisqvapaa ssdesvnqev lepvmrpepq dvlplqaegs ppageeavlv
      421 mpegqcpapa kkavirarqg gafkppalar qgssstetdy lrviyqllwy lwhalmqqna
      481 iqlhlppenl ellppdevgr hhaslrhrqg vaspaavgag ltlldapemq pcvtlapaap
      541 piphdrpasr astgpqvape atgceeeesv pdghcqtpvt rmvapplmrq splgreegtg
      601 rstaapapsl rvatvlapsp lladttlper tepsgstdgd qpqpvvtaar agedkeglpv
      661 prpkatstpt ghcsahhepp nvsvvlahgk hdskdlgdkg seshtrqeep regkqddrns
      721 rdfrdsrdfr esrdpvhsrd yrdsrdagqa rgrgsdgesl dwkgsqdnfe hdssrvgrgd
      781 lrddqdrkrd ryyeraddrr easkedrdey rkagrkigtt edrkrkddyy ddrradqled
      841 rredrregfr ddyrktdrrg dryhdrrdqw kgqesddewk errsdrrsdr ysrhdyrydd
      901 rhsdrysdrg rpssrtsqda yldeydqrvg drrvrdnhnr pssrhsesds sspdsrrgyg
      961 tgkhrkyrea drapgvyydh qksygtaggy dynayrrdyy nyyhygyapr sgygygyyde
     1021 lyrtnpaykq qvdayyarlg yspaqlmerl svhsgrssan gdsrrdesfa snteddcdps
     1081 ildnestrld hvpqpeevkk fsrphplarf cgangllklv ptlsggnlpp pieihslkhl
     1141 fqkdpqfhel kqfpgplvrs qthkndviqf cthkiqsfae dpnlpdrssh illwellvlm
     1201 lrqngfvsgs diaelllrgh nilepvvsas glarrggegg emtspegadd gsspspdegi
     1261 vvsdraqpga tgssqtlakf refllfghkk dalewavkcg lwghalslas kmdartyasi
     1321 mtrftsalat ndplqtlyqh lsgrqpaavt ciadekwgdw rphlamilsn pssrpevdar
     1381 sittlgdvla srgclsaahf cylmaqvefg sysckssklv llgsshqtll feefasneai
     1441 qcteiyeysq slanagymlp hlqaykflha trlaeygfaq ealhycevla eamgrqcgsa
     1501 qlaaqtyela srlkyhdphf sqgpgeleel geppwlaafa qlvtqllqqh aaaappqiga
     1561 lygqvsscgg navaevsggd syldqpgfts stgfsmhdsg msileqsgny gnsyldpsac
     1621 gdsaqavsps adqwtalaht apdsrsqhlp ptsvpsfaat gtsvesgwta vqpsaeysfv
     1681 stsppvesmv nggqtdgtap ssppvgsqtt tfdyykasty saqhpphhqt srerlssvss
     1741 stdahdrrds vdsaalasgr srlpsgpqsp lttdqsases ertkpaatnk gktqvagksw
     1801 lggifgkllp kgpnqmilpd dkdpdivwde hkkcwvdkna apgdadiqsa apptdssfgg
     1861 kptppsgkpg enrfqmakqr slrkhyvdvl npgsskasgd spavpgvppv peqpgtqspp
     1921 qflvpqavdd hcdsgydfvs papmpdhsap appppppetv syqqpsgkvv stpmmfdpad
     1981 fssravdssh kmaggnlarr raypt
//
LOCUS       XP_077501089            2002 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X10 [Amblyomma
            americanum].
ACCESSION   XP_077501089
VERSION     XP_077501089.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644963.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2002
                     /product="uncharacterized protein LOC144111913 isoform
                     X10"
                     /calculated_mol_wt=217065
     Region          <346..668
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          631..>865
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1102..>1176
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1150..1514
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1272..1273,1275..1276,1278..1282,1284,1286,1296,
                     1298,1300..1301,1303..1304,1308,1314,1321,1330,1335,1338,
                     1366..1367)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1439,1461..1462,1464..1465,1468..1469,1472,
                     1501..1502,1505,1509)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..2002
                     /gene="LOC144111913"
                     /coded_by="XM_077644963.1:24..6032"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 mdcwpvgatg kaaeaaqpak tlpqpvgevm pqwdgstsfq ptqypaalld pqqqqrqqqs
       61 nlqegwpsav tgaangstag savhcggpaq gdnywnwaah qepgaggdta asapmqswns
      121 wgaaqevwtg raqppdpatv ggtneaavap vvndgtslpp qlgytsppeg gqqqygsggv
      181 vaegalqegw aaphwsgdtn qwpsnleqqq fvtaqaqwqw qpadassavg qwqgygsqld
      241 paaagpyest slagyapldg slqqqqqqpg qdqlaassge nvagdmhlva yssgpaavdg
      301 qggsegtqhl eatlgdngmv smffqdggad dgqaesregg ptthdqhaee pgppshqqqt
      361 aatgiaqdai sqvapaassd esvnqevlep vmrpepqdvl plqaegsppa geeavlvmpe
      421 gqcpapakka virarqggaf kppalarqgs sstetdylrv iyqllwylwh almqqnaiql
      481 hlppenlell ppdevgrhha slrhrqgvas paavgagltl ldapemqpcv tlapaappip
      541 hdrpasrast gpqvapeatg ceeeesvpdg hcqtpvtrmv applmrqspl greegtgrst
      601 aapapslrva tvlapsplla dttlpertep sgstdgdqpq pvvtaarage dkeglpvprp
      661 katstptghc sahheppnvs vvlahgkhds kdlgdkgses htrqeepreg kqddrnsrdf
      721 rdsrdfresr dpvhsrdyrd srdagqargr gsdgesldwk gsqdnfehds srvgrgdlrd
      781 dqdrkrdryy eraddrreas kedrdeyrka grkigttedr krkddyyddr radqledrre
      841 drregfrddy rktdrrgdry hdrrdqwkgq esddewkerr sdrrsdrysr hdyryddrhs
      901 drysdrgrps srtsqdayld eydqrvgdrr vrdnhnrpss rhsesdsssp dsrrgygtgk
      961 hrkyreadra pgvyydhqks ygtaggydyn ayrrdyynyy hygyaprsgy gygyydelyr
     1021 tnpaykqqvd ayyarlgysp aqlmerlsvh sgrssangds rrdesfasnt eddcdpsild
     1081 nestrldhvp qpeevkkfsr phplarfcga ngllklvptl sggnlpppie ihslkhlfqk
     1141 dpqfhelkqf pgplvrsqth kndviqfcth kiqsfaedpn lpdrsshill wellvlmlrq
     1201 ngfvsgsdia elllrghnil epvvsasgla rrggeggemt spegaddgss pspdegivvs
     1261 draqpgatgs sqtlakfref llfghkkdal ewavkcglwg halslaskmd artyasimtr
     1321 ftsalatndp lqtlyqhlsg rqpaavtcia dekwgdwrph lamilsnpss rpevdarsit
     1381 tlgdvlasrg clsaahfcyl maqvefgsys ckssklvllg sshqtllfee fasneaiqct
     1441 eiyeysqsla nagymlphlq aykflhatrl aeygfaqeal hycevlaeam grqcgsaqla
     1501 aqtyelasrl kyhdphfsqg pgeleelgep pwlaafaqlv tqllqqhaaa appqigalyg
     1561 qvsscggnav aevsggdsyl dqpgftsstg fsmhdsgmsi leqsgnygns yldpsacgds
     1621 aqavspsadq wtalahtapd srsqhlppts vpsfaatgts vesgwtavqp saeysfvsts
     1681 ppvesmvngg qtdgtapssp pvgsqtttfd yykastysaq hpphhqtsre rlssvssstd
     1741 ahdrrdsvds aalasgrsrl psgpqspltt dqsasesert kpaatnkgkt qvagkswlgg
     1801 ifgkllpkgp nqmilpddkd pdivwdehkk cwvdknaapg dadiqsaapp tdssfggkpt
     1861 ppsgkpgenr fqmakqrslr khyvdvlnpg sskasgdspa vpgvppvpeq pgtqsppqfl
     1921 vpqavddhcd sgydfvspap mpdhsapapp ppppetvsyq qpsgkvvstp mmfdpadfss
     1981 ravdsshkma ggnlarrray pt
//
LOCUS       XP_077501090            2002 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X11 [Amblyomma
            americanum].
ACCESSION   XP_077501090
VERSION     XP_077501090.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644964.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2002
                     /product="uncharacterized protein LOC144111913 isoform
                     X11"
                     /calculated_mol_wt=217065
     Region          <345..667
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          630..>864
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1101..>1175
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1149..1513
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1271..1272,1274..1275,1277..1281,1283,1285,1295,
                     1297,1299..1300,1302..1303,1307,1313,1320,1329,1334,1337,
                     1365..1366)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1438,1460..1461,1463..1464,1467..1468,1471,
                     1500..1501,1504,1508)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..2002
                     /gene="LOC144111913"
                     /coded_by="XM_077644964.1:21..6029"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 mdcwpvgatg kaaeaaqpak tlpqpvgevm pqwdgstsfq ptqypaalld pqqqqrqqqs
       61 nlqegwpsav tgaangstag savhcggpaq gdnywnwaah qepgaggdta asapmqswns
      121 wgaaqevwtg raqppdpatv ggtneaavap vvndgtslpp qlgytsppeg gqqqygsggv
      181 vaegalqegw aaphwsgdtn qwpsnleqqq fvtaqaqwwq padassavgq wqgygsqldp
      241 aaagpyests lagyapldgs lqqqqqqpgq dqlaassgen vagdmhlvay ssgpaavdgq
      301 ggsegtqhle atlgdngmvs mffqdggadd gqaesreggp tthdqhaeep gppshqqqta
      361 atgiaqdais qvapaassde svnqevlepv mrpepqdvlp lqaegsppag eeavlvmpeg
      421 qcpapakkav irarqggafk ppalarqgss stetdylrvi yqllwylwha lmqqnaiqlh
      481 lppenlellp pdevgrhhas lrhrqgvasp aavgagltll dapemqpcvt lapaappiph
      541 drpasrastg pqvapeatgc eeeesvpdgh cqtpvtrmva pplmrqsplg reegtgrsta
      601 apapslrvat vlapspllad ttlperteps gstdgdqpqp vvtaaraged keglpvprpk
      661 atstptghcs ahheppnvsv vlahgkhdsk dlgdkgsesh trqeepregk qddrnsrdfr
      721 dsrdfresrd pvhsrdyrds rdagqargrg sdgesldwkg sqdnfehdss rvgrgdlrdd
      781 qdrkrdryye raddrreask edrdeyrkag rkigttedrk rkddyyddrr adqledrred
      841 rregfrddyr ktdrrgdryh drrdqwkgqe sddewkerrs drrsdrysrh dyryddrhsd
      901 rysdrgrpss rtsqdaylde ydqrvgdrrv rdnhnrpssr hsesdssspd srrgygtgkh
      961 rkyreadrap gvyydhqksy gtaggydyna yrrdyynyyh ygyaprsgyg ygyydelyrt
     1021 npaykqqvda yyarlgyspa qlmerlsvhs grssangdsr rdesfasnte ddcdpsildn
     1081 estrldhvpq peevkkfsrp hplarfcgan gllklvptls ggnlpppiei hslkhlfqkd
     1141 pqfhelkqfp gplvrsqthk ndviqfcthk iqsfaedpnl pdrsshillw ellvlmlrqn
     1201 gfvsgsdiae lllrghnile pvvsasglar rggeggemts pegaddgssp spdegivvsd
     1261 raqpgatgss qtlakfrefl lfghkkdale wavkcglwgh alslaskmda rtyasimtrf
     1321 tsalatndpl qtlyqhlsgr qpaavtciad ekwgdwrphl amilsnpssr pevdarsitt
     1381 lgdvlasrgc lsaahfcylm aqvefgsysc kssklvllgs shqtllfeef asneaiqcte
     1441 iyeysqslan agymlphlqa ykflhatrla eygfaqealh ycevlaeamg rqcgsaqlaa
     1501 qtyelasrlk yhdphfsqgp geleelgepp wlaafaqlvt qllqqhaaaa ppqigalygq
     1561 vsscggnava evsggdsyld qpgftsstgf smhdsgmsil eqsgnygnsy ldpsacgdsa
     1621 qavspsadqw talahtapds rsqhlpptsv psfaatgtsv esgwtavqps aeysfvstsp
     1681 pvesmvnggq tdgtapsspp vgsqtttfdy ykastysqaq hpphhqtsre rlssvssstd
     1741 ahdrrdsvds aalasgrsrl psgpqspltt dqsasesert kpaatnkgkt qvagkswlgg
     1801 ifgkllpkgp nqmilpddkd pdivwdehkk cwvdknaapg dadiqsaapp tdssfggkpt
     1861 ppsgkpgenr fqmakqrslr khyvdvlnpg sskasgdspa vpgvppvpeq pgtqsppqfl
     1921 vpqavddhcd sgydfvspap mpdhsapapp ppppetvsyq qpsgkvvstp mmfdpadfss
     1981 ravdsshkma ggnlarrray pt
//
LOCUS       XP_077501091            2001 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X12 [Amblyomma
            americanum].
ACCESSION   XP_077501091
VERSION     XP_077501091.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644965.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2001
                     /product="uncharacterized protein LOC144111913 isoform
                     X12"
                     /calculated_mol_wt=216937
     Region          <346..668
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          631..>865
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1102..>1176
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1150..1514
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1272..1273,1275..1276,1278..1282,1284,1286,1296,
                     1298,1300..1301,1303..1304,1308,1314,1321,1330,1335,1338,
                     1366..1367)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1439,1461..1462,1464..1465,1468..1469,1472,
                     1501..1502,1505,1509)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..2001
                     /gene="LOC144111913"
                     /coded_by="XM_077644965.1:21..6026"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 mdcwpvgatg kaaeaaqpak tlpqpvgevm pqwdgstsfq ptqypaalld pqqqqrqqqs
       61 nlqegwpsav tgaangstag savhcggpaq gdnywnwaah qepgaggdta asapmqswns
      121 wgaaqevwtg raqppdpatv ggtneaavap vvndgtslpp qlgytsppeg gqqqygsggv
      181 vaegalqegw aaphwsgdtn qwpsnleqqq fvtaqaqwqw qpadassavg qwqgygsqld
      241 paaagpyest slagyapldg slqqqqqqpg qdqlaassge nvagdmhlva yssgpaavdg
      301 qggsegtqhl eatlgdngmv smffqdggad dgqaesregg ptthdqhaee pgppshqqqt
      361 aatgiaqdai sqvapaassd esvnqevlep vmrpepqdvl plqaegsppa geeavlvmpe
      421 gqcpapakka virarqggaf kppalarqgs sstetdylrv iyqllwylwh almqqnaiql
      481 hlppenlell ppdevgrhha slrhrqgvas paavgagltl ldapemqpcv tlapaappip
      541 hdrpasrast gpqvapeatg ceeeesvpdg hcqtpvtrmv applmrqspl greegtgrst
      601 aapapslrva tvlapsplla dttlpertep sgstdgdqpq pvvtaarage dkeglpvprp
      661 katstptghc sahheppnvs vvlahgkhds kdlgdkgses htrqeepreg kqddrnsrdf
      721 rdsrdfresr dpvhsrdyrd srdagqargr gsdgesldwk gsqdnfehds srvgrgdlrd
      781 dqdrkrdryy eraddrreas kedrdeyrka grkigttedr krkddyyddr radqledrre
      841 drregfrddy rktdrrgdry hdrrdqwkgq esddewkerr sdrrsdrysr hdyryddrhs
      901 drysdrgrps srtsqdayld eydqrvgdrr vrdnhnrpss rhsesdsssp dsrrgygtgk
      961 hrkyreadra pgvyydhqks ygtaggydyn ayrrdyynyy hygyaprsgy gygyydelyr
     1021 tnpaykqqvd ayyarlgysp aqlmerlsvh sgrssangds rrdesfasnt eddcdpsild
     1081 nestrldhvp qpeevkkfsr phplarfcga ngllklvptl sggnlpppie ihslkhlfqk
     1141 dpqfhelkqf pgplvrsqth kndviqfcth kiqsfaedpn lpdrsshill wellvlmlrq
     1201 ngfvsgsdia elllrghnil epvvsasgla rrggeggemt spegaddgss pspdegivvs
     1261 draqpgatgs sqtlakfref llfghkkdal ewavkcglwg halslaskmd artyasimtr
     1321 ftsalatndp lqtlyqhlsg rqpaavtcia dekwgdwrph lamilsnpss rpevdarsit
     1381 tlgdvlasrg clsaahfcyl maqvefgsys ckssklvllg sshqtllfee fasneaiqct
     1441 eiyeysqsla nagymlphlq aykflhatrl aeygfaqeal hycevlaeam grqcgsaqla
     1501 aqtyelasrl kyhdphfsqg pgeleelgep pwlaafaqlv tqllqqhaaa appqigalyg
     1561 qvsscggnav aevsggdsyl dqpgftsstg fsmhdsgmsi leqsgnygns yldpsacgds
     1621 aqavspsadq wtalahtapd srsqhlppts vpsfaatgts vesgwtavqp saeysfvsts
     1681 ppvesmvngg qtdgtapssp pvgsqtttfd yykastysaq hpphhqtsre rlssvssstd
     1741 ahdrrdsvds aalasgrsrl psgpqspltt dqsasesert kpaatnkgkt qvagkswlgg
     1801 ifgkllpkgp nqmilpddkd pdivwdehkk cwvdknaapg dadiqsaapp tdssfggkpt
     1861 ppsgkpgenr fqmakqrslr khyvdvlnpg sskasgdspa vpgvppvpeq pgtqsppqfl
     1921 vpqavddhcd sgydfvspap mpdhsapapp ppppetvsyq psgkvvstpm mfdpadfssr
     1981 avdsshkmag gnlarrrayp t
//
LOCUS       XP_077501092            2000 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X13 [Amblyomma
            americanum].
ACCESSION   XP_077501092
VERSION     XP_077501092.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644966.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2000
                     /product="uncharacterized protein LOC144111913 isoform
                     X13"
                     /calculated_mol_wt=217131
     Region          <336..>746
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          629..>863
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1100..>1174
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1148..1512
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1270..1271,1273..1274,1276..1280,1282,1284,1294,
                     1296,1298..1299,1301..1302,1306,1312,1319,1328,1333,1336,
                     1364..1365)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1437,1459..1460,1462..1463,1466..1467,1470,
                     1499..1500,1503,1507)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..2000
                     /gene="LOC144111913"
                     /coded_by="XM_077644966.1:93..6095"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqegwp savtgaangs tagsavhcgg paqgdnywnw aahqepgagg dtaasapmqs
      121 wnswgaaqev wtgraqppdp atvggtneaa vapvvndgts lppqlgytsp peggqqqygs
      181 ggvvaegalq egwaaphwsg dtnqwpsnle qqqfvtaqaq wqwqpadass avgqwqgygs
      241 qldpaaagpy estslagyap ldgslqqqqq qpgqdqlaas sgenvagdmh lvayssgpaa
      301 vdgqggsegt qhleatlgdn gmvsmffqdg gaddgqaesr eggptthdqh aeepgppshq
      361 qqtaatgiaq daisqvapaa ssdesvnqev lepvmrpepq daegsppage eavlvmpegq
      421 cpapakkavi rarqggafkp palarqgsss tetdylrviy qllwylwhal mqqnaiqlhl
      481 ppenlellpp devgrhhasl rhrqgvaspa avgagltlld apemqpcvtl apaappiphd
      541 rpasrastgp qvapeatgce eeesvpdghc qtpvtrmvap plmrqsplgr eegtgrstaa
      601 papslrvatv lapsplladt tlpertepsg stdgdqpqpv vtaaragedk eglpvprpka
      661 tstptghcsa hheppnvsvv lahgkhdskd lgdkgsesht rqeepregkq ddrnsrdfrd
      721 srdfresrdp vhsrdyrdsr dagqargrgs dgesldwkgs qdnfehdssr vgrgdlrddq
      781 drkrdryyer addrreaske drdeyrkagr kigttedrkr kddyyddrra dqledrredr
      841 regfrddyrk tdrrgdryhd rrdqwkgqes ddewkerrsd rrsdrysrhd yryddrhsdr
      901 ysdrgrpssr tsqdayldey dqrvgdrrvr dnhnrpssrh sesdssspds rrgygtgkhr
      961 kyreadrapg vyydhqksyg taggydynay rrdyynyyhy gyaprsgygy gyydelyrtn
     1021 paykqqvday yarlgyspaq lmerlsvhsg rssangdsrr desfasnted dcdpsildne
     1081 strldhvpqp eevkkfsrph plarfcgang llklvptlsg gnlpppieih slkhlfqkdp
     1141 qfhelkqfpg plvrsqthkn dviqfcthki qsfaedpnlp drsshillwe llvlmlrqng
     1201 fvsgsdiael llrghnilep vvsasglarr ggeggemtsp egaddgssps pdegivvsdr
     1261 aqpgatgssq tlakfrefll fghkkdalew avkcglwgha lslaskmdar tyasimtrft
     1321 salatndplq tlyqhlsgrq paavtciade kwgdwrphla milsnpssrp evdarsittl
     1381 gdvlasrgcl saahfcylma qvefgsysck ssklvllgss hqtllfeefa sneaiqctei
     1441 yeysqslana gymlphlqay kflhatrlae ygfaqealhy cevlaeamgr qcgsaqlaaq
     1501 tyelasrlky hdphfsqgpg eleelgeppw laafaqlvtq llqqhaaaap pqigalygqv
     1561 sscggnavae vsggdsyldq pgftsstgfs mhdsgmsile qsgnygnsyl dpsacgdsaq
     1621 avspsadqwt alahtapdsr sqhlpptsvp sfaatgtsve sgwtavqpsa eysfvstspp
     1681 vesmvnggqt dgtapssppv gsqtttfdyy kastysaqhp phhqtsrerl ssvssstdah
     1741 drrdsvdsaa lasgrsrlps gpqsplttdq sasesertkp aatnkgktqv agkswlggif
     1801 gkllpkgpnq milpddkdpd ivwdehkkcw vdknaapgda diqsaapptd ssfggkptpp
     1861 sgkpgenrfq makqrslrkh yvdvlnpgss kasgdspavp gvppvpeqpg tqsppqflvp
     1921 qavddhcdsg ydfvspapmp dhsapapppp ppetvsyqqp sgkvvstpmm fdpadfssra
     1981 vdsshkmagg nlarrraypt
//
LOCUS       XP_077501093            1999 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X14 [Amblyomma
            americanum].
ACCESSION   XP_077501093
VERSION     XP_077501093.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644967.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1999
                     /product="uncharacterized protein LOC144111913 isoform
                     X14"
                     /calculated_mol_wt=216600
     Region          <357..664
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          627..>861
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1098..>1172
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1146..1510
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1268..1269,1271..1272,1274..1278,1280,1282,1292,
                     1294,1296..1297,1299..1300,1304,1310,1317,1326,1331,1334,
                     1362..1363)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1435,1457..1458,1460..1461,1464..1465,1468,
                     1497..1498,1501,1505)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1999
                     /gene="LOC144111913"
                     /coded_by="XM_077644967.1:93..6092"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqtccc lamqegwpsa vtgaangsta gsavhcggpa qgdnywnwaa hqepgaggdt
      121 aasapmqswn swgaaqevwt graqppdpat vggtneaava pvvndgtslp pqlgytsppe
      181 ggqqqygsgg vvaegalqeg waaphwsgdt nqwpsnleqq qfvtaqaqwq wqpadassav
      241 gqwqgygsql dpaaagpyes tslagyapld gslqqqqqqp gqdqlaassg envagdmhlv
      301 ayssgpaavd gqggsegtqh leatlgdngm vsmffqdgga ddgqaesreg gptthdqhae
      361 epgppshqqq taatgiaqda isqvapaass desvnqevle pvmrpepqdv lplqaegspp
      421 ageeavlvmp egqcpapakk avirarqgga fkppalarqg ssstetalmq qnaiqlhlpp
      481 enlellppde vgrhhaslrh rqgvaspaav gagltlldap emqpcvtlap aappiphdrp
      541 asrastgpqv apeatgceee esvpdghcqt pvtrmvappl mrqsplgree gtgrstaapa
      601 pslrvatvla psplladttl pertepsgst dgdqpqpvvt aaragedkeg lpvprpkats
      661 tptghcsahh eppnvsvvla hgkhdskdlg dkgseshtrq eepregkqdd rnsrdfrdsr
      721 dfresrdpvh srdyrdsrda gqargrgsdg esldwkgsqd nfehdssrvg rgdlrddqdr
      781 krdryyerad drreaskedr deyrkagrki gttedrkrkd dyyddrradq ledrredrre
      841 gfrddyrktd rrgdryhdrr dqwkgqesdd ewkerrsdrr sdrysrhdyr yddrhsdrys
      901 drgrpssrts qdayldeydq rvgdrrvrdn hnrpssrhse sdssspdsrr gygtgkhrky
      961 readrapgvy ydhqksygta ggydynayrr dyynyyhygy aprsgygygy ydelyrtnpa
     1021 ykqqvdayya rlgyspaqlm erlsvhsgrs sangdsrrde sfasnteddc dpsildnest
     1081 rldhvpqpee vkkfsrphpl arfcgangll klvptlsggn lpppieihsl khlfqkdpqf
     1141 helkqfpgpl vrsqthkndv iqfcthkiqs faedpnlpdr sshillwell vlmlrqngfv
     1201 sgsdiaelll rghnilepvv sasglarrgg eggemtspeg addgsspspd egivvsdraq
     1261 pgatgssqtl akfrefllfg hkkdalewav kcglwghals laskmdarty asimtrftsa
     1321 latndplqtl yqhlsgrqpa avtciadekw gdwrphlami lsnpssrpev darsittlgd
     1381 vlasrgclsa ahfcylmaqv efgsysckss klvllgsshq tllfeefasn eaiqcteiye
     1441 ysqslanagy mlphlqaykf lhatrlaeyg faqealhyce vlaeamgrqc gsaqlaaqty
     1501 elasrlkyhd phfsqgpgel eelgeppwla afaqlvtqll qqhaaaappq igalygqvss
     1561 cggnavaevs ggdsyldqpg ftsstgfsmh dsgmsileqs gnygnsyldp sacgdsaqav
     1621 spsadqwtal ahtapdsrsq hlpptsvpsf aatgtsvesg wtavqpsaey sfvstsppve
     1681 smvnggqtdg tapssppvgs qtttfdyyka stysqaqhpp hhqtsrerls svssstdahd
     1741 rrdsvdsaal asgrsrlpsg pqsplttdqs asesertkpa atnkgktqva gkswlggifg
     1801 kllpkgpnqm ilpddkdpdi vwdehkkcwv dknaapgdad iqsaapptds sfggkptpps
     1861 gkpgenrfqm akqrslrkhy vdvlnpgssk asgdspavpg vppvpeqpgt qsppqflvpq
     1921 avddhcdsgy dfvspapmpd hsapappppp petvsyqqps gkvvstpmmf dpadfssrav
     1981 dsshkmaggn larrraypt
//
LOCUS       XP_077501094            1998 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X15 [Amblyomma
            americanum].
ACCESSION   XP_077501094
VERSION     XP_077501094.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644968.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1998
                     /product="uncharacterized protein LOC144111913 isoform
                     X15"
                     /calculated_mol_wt=216472
     Region          <357..664
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          627..>861
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1098..>1172
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1146..1510
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1268..1269,1271..1272,1274..1278,1280,1282,1292,
                     1294,1296..1297,1299..1300,1304,1310,1317,1326,1331,1334,
                     1362..1363)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1435,1457..1458,1460..1461,1464..1465,1468,
                     1497..1498,1501,1505)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1998
                     /gene="LOC144111913"
                     /coded_by="XM_077644968.1:93..6089"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqtccc lamqegwpsa vtgaangsta gsavhcggpa qgdnywnwaa hqepgaggdt
      121 aasapmqswn swgaaqevwt graqppdpat vggtneaava pvvndgtslp pqlgytsppe
      181 ggqqqygsgg vvaegalqeg waaphwsgdt nqwpsnleqq qfvtaqaqwq wqpadassav
      241 gqwqgygsql dpaaagpyes tslagyapld gslqqqqqqp gqdqlaassg envagdmhlv
      301 ayssgpaavd gqggsegtqh leatlgdngm vsmffqdgga ddgqaesreg gptthdqhae
      361 epgppshqqq taatgiaqda isqvapaass desvnqevle pvmrpepqdv lplqaegspp
      421 ageeavlvmp egqcpapakk avirarqgga fkppalarqg ssstetalmq qnaiqlhlpp
      481 enlellppde vgrhhaslrh rqgvaspaav gagltlldap emqpcvtlap aappiphdrp
      541 asrastgpqv apeatgceee esvpdghcqt pvtrmvappl mrqsplgree gtgrstaapa
      601 pslrvatvla psplladttl pertepsgst dgdqpqpvvt aaragedkeg lpvprpkats
      661 tptghcsahh eppnvsvvla hgkhdskdlg dkgseshtrq eepregkqdd rnsrdfrdsr
      721 dfresrdpvh srdyrdsrda gqargrgsdg esldwkgsqd nfehdssrvg rgdlrddqdr
      781 krdryyerad drreaskedr deyrkagrki gttedrkrkd dyyddrradq ledrredrre
      841 gfrddyrktd rrgdryhdrr dqwkgqesdd ewkerrsdrr sdrysrhdyr yddrhsdrys
      901 drgrpssrts qdayldeydq rvgdrrvrdn hnrpssrhse sdssspdsrr gygtgkhrky
      961 readrapgvy ydhqksygta ggydynayrr dyynyyhygy aprsgygygy ydelyrtnpa
     1021 ykqqvdayya rlgyspaqlm erlsvhsgrs sangdsrrde sfasnteddc dpsildnest
     1081 rldhvpqpee vkkfsrphpl arfcgangll klvptlsggn lpppieihsl khlfqkdpqf
     1141 helkqfpgpl vrsqthkndv iqfcthkiqs faedpnlpdr sshillwell vlmlrqngfv
     1201 sgsdiaelll rghnilepvv sasglarrgg eggemtspeg addgsspspd egivvsdraq
     1261 pgatgssqtl akfrefllfg hkkdalewav kcglwghals laskmdarty asimtrftsa
     1321 latndplqtl yqhlsgrqpa avtciadekw gdwrphlami lsnpssrpev darsittlgd
     1381 vlasrgclsa ahfcylmaqv efgsysckss klvllgsshq tllfeefasn eaiqcteiye
     1441 ysqslanagy mlphlqaykf lhatrlaeyg faqealhyce vlaeamgrqc gsaqlaaqty
     1501 elasrlkyhd phfsqgpgel eelgeppwla afaqlvtqll qqhaaaappq igalygqvss
     1561 cggnavaevs ggdsyldqpg ftsstgfsmh dsgmsileqs gnygnsyldp sacgdsaqav
     1621 spsadqwtal ahtapdsrsq hlpptsvpsf aatgtsvesg wtavqpsaey sfvstsppve
     1681 smvnggqtdg tapssppvgs qtttfdyyka stysaqhpph hqtsrerlss vssstdahdr
     1741 rdsvdsaala sgrsrlpsgp qsplttdqsa sesertkpaa tnkgktqvag kswlggifgk
     1801 llpkgpnqmi lpddkdpdiv wdehkkcwvd knaapgdadi qsaapptdss fggkptppsg
     1861 kpgenrfqma kqrslrkhyv dvlnpgsska sgdspavpgv ppvpeqpgtq sppqflvpqa
     1921 vddhcdsgyd fvspapmpdh sapapppppp etvsyqqpsg kvvstpmmfd padfssravd
     1981 sshkmaggnl arrraypt
//
LOCUS       XP_077501095            1997 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X16 [Amblyomma
            americanum].
ACCESSION   XP_077501095
VERSION     XP_077501095.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644969.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1997
                     /product="uncharacterized protein LOC144111913 isoform
                     X16"
                     /calculated_mol_wt=216344
     Region          <357..664
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          627..>861
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1098..>1172
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1146..1510
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1268..1269,1271..1272,1274..1278,1280,1282,1292,
                     1294,1296..1297,1299..1300,1304,1310,1317,1326,1331,1334,
                     1362..1363)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1435,1457..1458,1460..1461,1464..1465,1468,
                     1497..1498,1501,1505)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1997
                     /gene="LOC144111913"
                     /coded_by="XM_077644969.1:93..6086"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqtccc lamqegwpsa vtgaangsta gsavhcggpa qgdnywnwaa hqepgaggdt
      121 aasapmqswn swgaaqevwt graqppdpat vggtneaava pvvndgtslp pqlgytsppe
      181 ggqqqygsgg vvaegalqeg waaphwsgdt nqwpsnleqq qfvtaqaqwq wqpadassav
      241 gqwqgygsql dpaaagpyes tslagyapld gslqqqqqqp gqdqlaassg envagdmhlv
      301 ayssgpaavd gqggsegtqh leatlgdngm vsmffqdgga ddgqaesreg gptthdqhae
      361 epgppshqqq taatgiaqda isqvapaass desvnqevle pvmrpepqdv lplqaegspp
      421 ageeavlvmp egqcpapakk avirarqgga fkppalarqg ssstetalmq qnaiqlhlpp
      481 enlellppde vgrhhaslrh rqgvaspaav gagltlldap emqpcvtlap aappiphdrp
      541 asrastgpqv apeatgceee esvpdghcqt pvtrmvappl mrqsplgree gtgrstaapa
      601 pslrvatvla psplladttl pertepsgst dgdqpqpvvt aaragedkeg lpvprpkats
      661 tptghcsahh eppnvsvvla hgkhdskdlg dkgseshtrq eepregkqdd rnsrdfrdsr
      721 dfresrdpvh srdyrdsrda gqargrgsdg esldwkgsqd nfehdssrvg rgdlrddqdr
      781 krdryyerad drreaskedr deyrkagrki gttedrkrkd dyyddrradq ledrredrre
      841 gfrddyrktd rrgdryhdrr dqwkgqesdd ewkerrsdrr sdrysrhdyr yddrhsdrys
      901 drgrpssrts qdayldeydq rvgdrrvrdn hnrpssrhse sdssspdsrr gygtgkhrky
      961 readrapgvy ydhqksygta ggydynayrr dyynyyhygy aprsgygygy ydelyrtnpa
     1021 ykqqvdayya rlgyspaqlm erlsvhsgrs sangdsrrde sfasnteddc dpsildnest
     1081 rldhvpqpee vkkfsrphpl arfcgangll klvptlsggn lpppieihsl khlfqkdpqf
     1141 helkqfpgpl vrsqthkndv iqfcthkiqs faedpnlpdr sshillwell vlmlrqngfv
     1201 sgsdiaelll rghnilepvv sasglarrgg eggemtspeg addgsspspd egivvsdraq
     1261 pgatgssqtl akfrefllfg hkkdalewav kcglwghals laskmdarty asimtrftsa
     1321 latndplqtl yqhlsgrqpa avtciadekw gdwrphlami lsnpssrpev darsittlgd
     1381 vlasrgclsa ahfcylmaqv efgsysckss klvllgsshq tllfeefasn eaiqcteiye
     1441 ysqslanagy mlphlqaykf lhatrlaeyg faqealhyce vlaeamgrqc gsaqlaaqty
     1501 elasrlkyhd phfsqgpgel eelgeppwla afaqlvtqll qqhaaaappq igalygqvss
     1561 cggnavaevs ggdsyldqpg ftsstgfsmh dsgmsileqs gnygnsyldp sacgdsaqav
     1621 spsadqwtal ahtapdsrsq hlpptsvpsf aatgtsvesg wtavqpsaey sfvstsppve
     1681 smvnggqtdg tapssppvgs qtttfdyyka stysaqhpph hqtsrerlss vssstdahdr
     1741 rdsvdsaala sgrsrlpsgp qsplttdqsa sesertkpaa tnkgktqvag kswlggifgk
     1801 llpkgpnqmi lpddkdpdiv wdehkkcwvd knaapgdadi qsaapptdss fggkptppsg
     1861 kpgenrfqma kqrslrkhyv dvlnpgsska sgdspavpgv ppvpeqpgtq sppqflvpqa
     1921 vddhcdsgyd fvspapmpdh sapapppppp etvsyqpsgk vvstpmmfdp adfssravds
     1981 shkmaggnla rrraypt
//
LOCUS       XP_077501096            1996 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X17 [Amblyomma
            americanum].
ACCESSION   XP_077501096
VERSION     XP_077501096.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644970.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1996
                     /product="uncharacterized protein LOC144111913 isoform
                     X17"
                     /calculated_mol_wt=216216
     Region          <356..663
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          626..>860
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1097..>1171
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1145..1509
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1267..1268,1270..1271,1273..1277,1279,1281,1291,
                     1293,1295..1296,1298..1299,1303,1309,1316,1325,1330,1333,
                     1361..1362)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1434,1456..1457,1459..1460,1463..1464,1467,
                     1496..1497,1500,1504)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1996
                     /gene="LOC144111913"
                     /coded_by="XM_077644970.1:93..6083"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqtccc lamqegwpsa vtgaangsta gsavhcggpa qgdnywnwaa hqepgaggdt
      121 aasapmqswn swgaaqevwt graqppdpat vggtneaava pvvndgtslp pqlgytsppe
      181 ggqqqygsgg vvaegalqeg waaphwsgdt nqwpsnleqq qfvtaqaqww qpadassavg
      241 qwqgygsqld paaagpyest slagyapldg slqqqqqqpg qdqlaassge nvagdmhlva
      301 yssgpaavdg qggsegtqhl eatlgdngmv smffqdggad dgqaesregg ptthdqhaee
      361 pgppshqqqt aatgiaqdai sqvapaassd esvnqevlep vmrpepqdvl plqaegsppa
      421 geeavlvmpe gqcpapakka virarqggaf kppalarqgs sstetalmqq naiqlhlppe
      481 nlellppdev grhhaslrhr qgvaspaavg agltlldape mqpcvtlapa appiphdrpa
      541 srastgpqva peatgceeee svpdghcqtp vtrmvapplm rqsplgreeg tgrstaapap
      601 slrvatvlap splladttlp ertepsgstd gdqpqpvvta aragedkegl pvprpkatst
      661 ptghcsahhe ppnvsvvlah gkhdskdlgd kgseshtrqe epregkqddr nsrdfrdsrd
      721 fresrdpvhs rdyrdsrdag qargrgsdge sldwkgsqdn fehdssrvgr gdlrddqdrk
      781 rdryyeradd rreaskedrd eyrkagrkig ttedrkrkdd yyddrradql edrredrreg
      841 frddyrktdr rgdryhdrrd qwkgqesdde wkerrsdrrs drysrhdyry ddrhsdrysd
      901 rgrpssrtsq dayldeydqr vgdrrvrdnh nrpssrhses dssspdsrrg ygtgkhrkyr
      961 eadrapgvyy dhqksygtag gydynayrrd yynyyhygya prsgygygyy delyrtnpay
     1021 kqqvdayyar lgyspaqlme rlsvhsgrss angdsrrdes fasnteddcd psildnestr
     1081 ldhvpqpeev kkfsrphpla rfcgangllk lvptlsggnl pppieihslk hlfqkdpqfh
     1141 elkqfpgplv rsqthkndvi qfcthkiqsf aedpnlpdrs shillwellv lmlrqngfvs
     1201 gsdiaelllr ghnilepvvs asglarrgge ggemtspega ddgsspspde givvsdraqp
     1261 gatgssqtla kfrefllfgh kkdalewavk cglwghalsl askmdartya simtrftsal
     1321 atndplqtly qhlsgrqpaa vtciadekwg dwrphlamil snpssrpevd arsittlgdv
     1381 lasrgclsaa hfcylmaqve fgsysckssk lvllgsshqt llfeefasne aiqcteiyey
     1441 sqslanagym lphlqaykfl hatrlaeygf aqealhycev laeamgrqcg saqlaaqtye
     1501 lasrlkyhdp hfsqgpgele elgeppwlaa faqlvtqllq qhaaaappqi galygqvssc
     1561 ggnavaevsg gdsyldqpgf tsstgfsmhd sgmsileqsg nygnsyldps acgdsaqavs
     1621 psadqwtala htapdsrsqh lpptsvpsfa atgtsvesgw tavqpsaeys fvstsppves
     1681 mvnggqtdgt apssppvgsq tttfdyykas tysaqhpphh qtsrerlssv ssstdahdrr
     1741 dsvdsaalas grsrlpsgpq splttdqsas esertkpaat nkgktqvagk swlggifgkl
     1801 lpkgpnqmil pddkdpdivw dehkkcwvdk naapgdadiq saapptdssf ggkptppsgk
     1861 pgenrfqmak qrslrkhyvd vlnpgsskas gdspavpgvp pvpeqpgtqs ppqflvpqav
     1921 ddhcdsgydf vspapmpdhs apappppppe tvsyqpsgkv vstpmmfdpa dfssravdss
     1981 hkmaggnlar rraypt
//
LOCUS       XP_077501097            1993 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X18 [Amblyomma
            americanum].
ACCESSION   XP_077501097
VERSION     XP_077501097.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644971.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1993
                     /product="uncharacterized protein LOC144111913 isoform
                     X18"
                     /calculated_mol_wt=215921
     Region          <357..659
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          622..>856
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1093..>1167
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1141..1505
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1263..1264,1266..1267,1269..1273,1275,1277,1287,
                     1289,1291..1292,1294..1295,1299,1305,1312,1321,1326,1329,
                     1357..1358)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1430,1452..1453,1455..1456,1459..1460,1463,
                     1492..1493,1496,1500)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1993
                     /gene="LOC144111913"
                     /coded_by="XM_077644971.1:93..6074"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqtccc lamqegwpsa vtgaangsta gsavhcggpa qgdnywnwaa hqepgaggdt
      121 aasapmqswn swgaaqevwt graqppdpat vggtneaava pvvndgtslp pqlgytsppe
      181 ggqqqygsgg vvaegalqeg waaphwsgdt nqwpsnleqq qfvtaqaqwq wqpadassav
      241 gqwqgygsql dpaaagpyes tslagyapld gslqqqqqqp gqdqlaassg envagdmhlv
      301 ayssgpaavd gqggsegtqh leatlgdngm vsmffqdgga ddgqaesreg gptthdqhae
      361 epgppshqqq taatgiaqda isqvapaass desvnqevle pvmrpepqda egsppageea
      421 vlvmpegqcp apakkavira rqggafkppa larqgssste talmqqnaiq lhlppenlel
      481 lppdevgrhh aslrhrqgva spaavgaglt lldapemqpc vtlapaappi phdrpasras
      541 tgpqvapeat gceeeesvpd ghcqtpvtrm vapplmrqsp lgreegtgrs taapapslrv
      601 atvlapspll adttlperte psgstdgdqp qpvvtaarag edkeglpvpr pkatstptgh
      661 csahheppnv svvlahgkhd skdlgdkgse shtrqeepre gkqddrnsrd frdsrdfres
      721 rdpvhsrdyr dsrdagqarg rgsdgesldw kgsqdnfehd ssrvgrgdlr ddqdrkrdry
      781 yeraddrrea skedrdeyrk agrkigtted rkrkddyydd rradqledrr edrregfrdd
      841 yrktdrrgdr yhdrrdqwkg qesddewker rsdrrsdrys rhdyryddrh sdrysdrgrp
      901 ssrtsqdayl deydqrvgdr rvrdnhnrps srhsesdsss pdsrrgygtg khrkyreadr
      961 apgvyydhqk sygtaggydy nayrrdyyny yhygyaprsg ygygyydely rtnpaykqqv
     1021 dayyarlgys paqlmerlsv hsgrssangd srrdesfasn teddcdpsil dnestrldhv
     1081 pqpeevkkfs rphplarfcg angllklvpt lsggnlpppi eihslkhlfq kdpqfhelkq
     1141 fpgplvrsqt hkndviqfct hkiqsfaedp nlpdrsshil lwellvlmlr qngfvsgsdi
     1201 aelllrghni lepvvsasgl arrggeggem tspegaddgs spspdegivv sdraqpgatg
     1261 ssqtlakfre fllfghkkda lewavkcglw ghalslaskm dartyasimt rftsalatnd
     1321 plqtlyqhls grqpaavtci adekwgdwrp hlamilsnps srpevdarsi ttlgdvlasr
     1381 gclsaahfcy lmaqvefgsy sckssklvll gsshqtllfe efasneaiqc teiyeysqsl
     1441 anagymlphl qaykflhatr laeygfaqea lhycevlaea mgrqcgsaql aaqtyelasr
     1501 lkyhdphfsq gpgeleelge ppwlaafaql vtqllqqhaa aappqigaly gqvsscggna
     1561 vaevsggdsy ldqpgftsst gfsmhdsgms ileqsgnygn syldpsacgd saqavspsad
     1621 qwtalahtap dsrsqhlppt svpsfaatgt svesgwtavq psaeysfvst sppvesmvng
     1681 gqtdgtapss ppvgsqtttf dyykastysa qhpphhqtsr erlssvssst dahdrrdsvd
     1741 saalasgrsr lpsgpqsplt tdqsaseser tkpaatnkgk tqvagkswlg gifgkllpkg
     1801 pnqmilpddk dpdivwdehk kcwvdknaap gdadiqsaap ptdssfggkp tppsgkpgen
     1861 rfqmakqrsl rkhyvdvlnp gsskasgdsp avpgvppvpe qpgtqsppqf lvpqavddhc
     1921 dsgydfvspa pmpdhsapap pppppetvsy qqpsgkvvst pmmfdpadfs sravdsshkm
     1981 aggnlarrra ypt
//
LOCUS       XP_077501098            1992 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X19 [Amblyomma
            americanum].
ACCESSION   XP_077501098
VERSION     XP_077501098.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644972.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1992
                     /product="uncharacterized protein LOC144111913 isoform
                     X19"
                     /calculated_mol_wt=216576
     Region          <335..657
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          620..>854
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1091..>1165
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1139..1503
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1261..1262,1264..1265,1267..1271,1273,1275,1285,
                     1287,1289..1290,1292..1293,1297,1303,1310,1319,1324,1327,
                     1355..1356)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1428,1450..1451,1453..1454,1457..1458,1461,
                     1490..1491,1494,1498)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1992
                     /gene="LOC144111913"
                     /coded_by="XM_077644972.1:93..6071"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqtccc lamqegwpsa vtgaangsta gsavhcggpa qgdnywnwaa hqepgaggdt
      121 aasapmqswn swgaaqevwt graqppdpat vggtneaava pvvndgtslp pqlgytsppe
      181 ggqqqygsgg vvaegalqeg waaphwsgdt nqwpsnleqq qfvtaqaqwq wqpadassav
      241 gqwqgygsql dpaaagpyes tslagyapld gslqqqqqqp gqdqlaassg envagtqhle
      301 atlgdngmvs mffqdggadd gqaesreggp tthdqhaeep gppshqqqta atgiaqdais
      361 qvapaassde svnqevlepv mrpepqdvlp lqaegsppag eeavlvmpeg qcpapakkav
      421 irarqggafk ppalarqgss stetdylrvi yqllwylwha lmqqnaiqlh lppenlellp
      481 pdevgrhhas lrhrqgvasp aavgagltll dapemqpcvt lapaappiph drpasrastg
      541 pqvapeatgc eeeesvpdgh cqtpvtrmva pplmrqsplg reegtgrsta apapslrvat
      601 vlapspllad ttlperteps gstdgdqpqp vvtaaraged keglpvprpk atstptghcs
      661 ahheppnvsv vlahgkhdsk dlgdkgsesh trqeepregk qddrnsrdfr dsrdfresrd
      721 pvhsrdyrds rdagqargrg sdgesldwkg sqdnfehdss rvgrgdlrdd qdrkrdryye
      781 raddrreask edrdeyrkag rkigttedrk rkddyyddrr adqledrred rregfrddyr
      841 ktdrrgdryh drrdqwkgqe sddewkerrs drrsdrysrh dyryddrhsd rysdrgrpss
      901 rtsqdaylde ydqrvgdrrv rdnhnrpssr hsesdssspd srrgygtgkh rkyreadrap
      961 gvyydhqksy gtaggydyna yrrdyynyyh ygyaprsgyg ygyydelyrt npaykqqvda
     1021 yyarlgyspa qlmerlsvhs grssangdsr rdesfasnte ddcdpsildn estrldhvpq
     1081 peevkkfsrp hplarfcgan gllklvptls ggnlpppiei hslkhlfqkd pqfhelkqfp
     1141 gplvrsqthk ndviqfcthk iqsfaedpnl pdrsshillw ellvlmlrqn gfvsgsdiae
     1201 lllrghnile pvvsasglar rggeggemts pegaddgssp spdegivvsd raqpgatgss
     1261 qtlakfrefl lfghkkdale wavkcglwgh alslaskmda rtyasimtrf tsalatndpl
     1321 qtlyqhlsgr qpaavtciad ekwgdwrphl amilsnpssr pevdarsitt lgdvlasrgc
     1381 lsaahfcylm aqvefgsysc kssklvllgs shqtllfeef asneaiqcte iyeysqslan
     1441 agymlphlqa ykflhatrla eygfaqealh ycevlaeamg rqcgsaqlaa qtyelasrlk
     1501 yhdphfsqgp geleelgepp wlaafaqlvt qllqqhaaaa ppqigalygq vsscggnava
     1561 evsggdsyld qpgftsstgf smhdsgmsil eqsgnygnsy ldpsacgdsa qavspsadqw
     1621 talahtapds rsqhlpptsv psfaatgtsv esgwtavqps aeysfvstsp pvesmvnggq
     1681 tdgtapsspp vgsqtttfdy ykastysqaq hpphhqtsre rlssvssstd ahdrrdsvds
     1741 aalasgrsrl psgpqspltt dqsasesert kpaatnkgkt qvagkswlgg ifgkllpkgp
     1801 nqmilpddkd pdivwdehkk cwvdknaapg dadiqsaapp tdssfggkpt ppsgkpgenr
     1861 fqmakqrslr khyvdvlnpg sskasgdspa vpgvppvpeq pgtqsppqfl vpqavddhcd
     1921 sgydfvspap mpdhsapapp ppppetvsyq qpsgkvvstp mmfdpadfss ravdsshkma
     1981 ggnlarrray pt
//
LOCUS       XP_077501099            1992 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X20 [Amblyomma
            americanum].
ACCESSION   XP_077501099
VERSION     XP_077501099.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644973.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1992
                     /product="uncharacterized protein LOC144111913 isoform
                     X20"
                     /calculated_mol_wt=215793
     Region          <357..659
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          622..>856
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1093..>1167
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1141..1505
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1263..1264,1266..1267,1269..1273,1275,1277,1287,
                     1289,1291..1292,1294..1295,1299,1305,1312,1321,1326,1329,
                     1357..1358)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1430,1452..1453,1455..1456,1459..1460,1463,
                     1492..1493,1496,1500)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1992
                     /gene="LOC144111913"
                     /coded_by="XM_077644973.1:93..6071"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqtccc lamqegwpsa vtgaangsta gsavhcggpa qgdnywnwaa hqepgaggdt
      121 aasapmqswn swgaaqevwt graqppdpat vggtneaava pvvndgtslp pqlgytsppe
      181 ggqqqygsgg vvaegalqeg waaphwsgdt nqwpsnleqq qfvtaqaqwq wqpadassav
      241 gqwqgygsql dpaaagpyes tslagyapld gslqqqqqqp gqdqlaassg envagdmhlv
      301 ayssgpaavd gqggsegtqh leatlgdngm vsmffqdgga ddgqaesreg gptthdqhae
      361 epgppshqqq taatgiaqda isqvapaass desvnqevle pvmrpepqda egsppageea
      421 vlvmpegqcp apakkavira rqggafkppa larqgssste talmqqnaiq lhlppenlel
      481 lppdevgrhh aslrhrqgva spaavgaglt lldapemqpc vtlapaappi phdrpasras
      541 tgpqvapeat gceeeesvpd ghcqtpvtrm vapplmrqsp lgreegtgrs taapapslrv
      601 atvlapspll adttlperte psgstdgdqp qpvvtaarag edkeglpvpr pkatstptgh
      661 csahheppnv svvlahgkhd skdlgdkgse shtrqeepre gkqddrnsrd frdsrdfres
      721 rdpvhsrdyr dsrdagqarg rgsdgesldw kgsqdnfehd ssrvgrgdlr ddqdrkrdry
      781 yeraddrrea skedrdeyrk agrkigtted rkrkddyydd rradqledrr edrregfrdd
      841 yrktdrrgdr yhdrrdqwkg qesddewker rsdrrsdrys rhdyryddrh sdrysdrgrp
      901 ssrtsqdayl deydqrvgdr rvrdnhnrps srhsesdsss pdsrrgygtg khrkyreadr
      961 apgvyydhqk sygtaggydy nayrrdyyny yhygyaprsg ygygyydely rtnpaykqqv
     1021 dayyarlgys paqlmerlsv hsgrssangd srrdesfasn teddcdpsil dnestrldhv
     1081 pqpeevkkfs rphplarfcg angllklvpt lsggnlpppi eihslkhlfq kdpqfhelkq
     1141 fpgplvrsqt hkndviqfct hkiqsfaedp nlpdrsshil lwellvlmlr qngfvsgsdi
     1201 aelllrghni lepvvsasgl arrggeggem tspegaddgs spspdegivv sdraqpgatg
     1261 ssqtlakfre fllfghkkda lewavkcglw ghalslaskm dartyasimt rftsalatnd
     1321 plqtlyqhls grqpaavtci adekwgdwrp hlamilsnps srpevdarsi ttlgdvlasr
     1381 gclsaahfcy lmaqvefgsy sckssklvll gsshqtllfe efasneaiqc teiyeysqsl
     1441 anagymlphl qaykflhatr laeygfaqea lhycevlaea mgrqcgsaql aaqtyelasr
     1501 lkyhdphfsq gpgeleelge ppwlaafaql vtqllqqhaa aappqigaly gqvsscggna
     1561 vaevsggdsy ldqpgftsst gfsmhdsgms ileqsgnygn syldpsacgd saqavspsad
     1621 qwtalahtap dsrsqhlppt svpsfaatgt svesgwtavq psaeysfvst sppvesmvng
     1681 gqtdgtapss ppvgsqtttf dyykastysa qhpphhqtsr erlssvssst dahdrrdsvd
     1741 saalasgrsr lpsgpqsplt tdqsaseser tkpaatnkgk tqvagkswlg gifgkllpkg
     1801 pnqmilpddk dpdivwdehk kcwvdknaap gdadiqsaap ptdssfggkp tppsgkpgen
     1861 rfqmakqrsl rkhyvdvlnp gsskasgdsp avpgvppvpe qpgtqsppqf lvpqavddhc
     1921 dsgydfvspa pmpdhsapap pppppetvsy qpsgkvvstp mmfdpadfss ravdsshkma
     1981 ggnlarrray pt
//
LOCUS       XP_077501100            1991 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X21 [Amblyomma
            americanum].
ACCESSION   XP_077501100
VERSION     XP_077501100.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644974.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1991
                     /product="uncharacterized protein LOC144111913 isoform
                     X21"
                     /calculated_mol_wt=215746
     Region          <349..656
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          619..>853
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1090..>1164
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1138..1502
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1260..1261,1263..1264,1266..1270,1272,1274,1284,
                     1286,1288..1289,1291..1292,1296,1302,1309,1318,1323,1326,
                     1354..1355)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1427,1449..1450,1452..1453,1456..1457,1460,
                     1489..1490,1493,1497)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1991
                     /gene="LOC144111913"
                     /coded_by="XM_077644974.1:93..6068"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqegwp savtgaangs tagsavhcgg paqgdnywnw aahqepgagg dtaasapmqs
      121 wnswgaaqev wtgraqppdp atvggtneaa vapvvndgts lppqlgytsp peggqqqygs
      181 ggvvaegalq egwaaphwsg dtnqwpsnle qqqfvtaqaq wqwqpadass avgqwqgygs
      241 qldpaaagpy estslagyap ldgslqqqqq qpgqdqlaas sgenvagdmh lvayssgpaa
      301 vdgqggsegt qhleatlgdn gmvsmffqdg gaddgqaesr eggptthdqh aeepgppshq
      361 qqtaatgiaq daisqvapaa ssdesvnqev lepvmrpepq dvlplqaegs ppageeavlv
      421 mpegqcpapa kkavirarqg gafkppalar qgssstetal mqqnaiqlhl ppenlellpp
      481 devgrhhasl rhrqgvaspa avgagltlld apemqpcvtl apaappiphd rpasrastgp
      541 qvapeatgce eeesvpdghc qtpvtrmvap plmrqsplgr eegtgrstaa papslrvatv
      601 lapsplladt tlpertepsg stdgdqpqpv vtaaragedk eglpvprpka tstptghcsa
      661 hheppnvsvv lahgkhdskd lgdkgsesht rqeepregkq ddrnsrdfrd srdfresrdp
      721 vhsrdyrdsr dagqargrgs dgesldwkgs qdnfehdssr vgrgdlrddq drkrdryyer
      781 addrreaske drdeyrkagr kigttedrkr kddyyddrra dqledrredr regfrddyrk
      841 tdrrgdryhd rrdqwkgqes ddewkerrsd rrsdrysrhd yryddrhsdr ysdrgrpssr
      901 tsqdayldey dqrvgdrrvr dnhnrpssrh sesdssspds rrgygtgkhr kyreadrapg
      961 vyydhqksyg taggydynay rrdyynyyhy gyaprsgygy gyydelyrtn paykqqvday
     1021 yarlgyspaq lmerlsvhsg rssangdsrr desfasnted dcdpsildne strldhvpqp
     1081 eevkkfsrph plarfcgang llklvptlsg gnlpppieih slkhlfqkdp qfhelkqfpg
     1141 plvrsqthkn dviqfcthki qsfaedpnlp drsshillwe llvlmlrqng fvsgsdiael
     1201 llrghnilep vvsasglarr ggeggemtsp egaddgssps pdegivvsdr aqpgatgssq
     1261 tlakfrefll fghkkdalew avkcglwgha lslaskmdar tyasimtrft salatndplq
     1321 tlyqhlsgrq paavtciade kwgdwrphla milsnpssrp evdarsittl gdvlasrgcl
     1381 saahfcylma qvefgsysck ssklvllgss hqtllfeefa sneaiqctei yeysqslana
     1441 gymlphlqay kflhatrlae ygfaqealhy cevlaeamgr qcgsaqlaaq tyelasrlky
     1501 hdphfsqgpg eleelgeppw laafaqlvtq llqqhaaaap pqigalygqv sscggnavae
     1561 vsggdsyldq pgftsstgfs mhdsgmsile qsgnygnsyl dpsacgdsaq avspsadqwt
     1621 alahtapdsr sqhlpptsvp sfaatgtsve sgwtavqpsa eysfvstspp vesmvnggqt
     1681 dgtapssppv gsqtttfdyy kastysqaqh pphhqtsrer lssvssstda hdrrdsvdsa
     1741 alasgrsrlp sgpqsplttd qsasesertk paatnkgktq vagkswlggi fgkllpkgpn
     1801 qmilpddkdp divwdehkkc wvdknaapgd adiqsaappt dssfggkptp psgkpgenrf
     1861 qmakqrslrk hyvdvlnpgs skasgdspav pgvppvpeqp gtqsppqflv pqavddhcds
     1921 gydfvspapm pdhsapappp pppetvsyqq psgkvvstpm mfdpadfssr avdsshkmag
     1981 gnlarrrayp t
//
LOCUS       XP_077501101            1990 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X22 [Amblyomma
            americanum].
ACCESSION   XP_077501101
VERSION     XP_077501101.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644975.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1990
                     /product="uncharacterized protein LOC144111913 isoform
                     X22"
                     /calculated_mol_wt=215618
     Region          <349..656
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          619..>853
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1090..>1164
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1138..1502
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1260..1261,1263..1264,1266..1270,1272,1274,1284,
                     1286,1288..1289,1291..1292,1296,1302,1309,1318,1323,1326,
                     1354..1355)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1427,1449..1450,1452..1453,1456..1457,1460,
                     1489..1490,1493,1497)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1990
                     /gene="LOC144111913"
                     /coded_by="XM_077644975.1:93..6065"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqegwp savtgaangs tagsavhcgg paqgdnywnw aahqepgagg dtaasapmqs
      121 wnswgaaqev wtgraqppdp atvggtneaa vapvvndgts lppqlgytsp peggqqqygs
      181 ggvvaegalq egwaaphwsg dtnqwpsnle qqqfvtaqaq wqwqpadass avgqwqgygs
      241 qldpaaagpy estslagyap ldgslqqqqq qpgqdqlaas sgenvagdmh lvayssgpaa
      301 vdgqggsegt qhleatlgdn gmvsmffqdg gaddgqaesr eggptthdqh aeepgppshq
      361 qqtaatgiaq daisqvapaa ssdesvnqev lepvmrpepq dvlplqaegs ppageeavlv
      421 mpegqcpapa kkavirarqg gafkppalar qgssstetal mqqnaiqlhl ppenlellpp
      481 devgrhhasl rhrqgvaspa avgagltlld apemqpcvtl apaappiphd rpasrastgp
      541 qvapeatgce eeesvpdghc qtpvtrmvap plmrqsplgr eegtgrstaa papslrvatv
      601 lapsplladt tlpertepsg stdgdqpqpv vtaaragedk eglpvprpka tstptghcsa
      661 hheppnvsvv lahgkhdskd lgdkgsesht rqeepregkq ddrnsrdfrd srdfresrdp
      721 vhsrdyrdsr dagqargrgs dgesldwkgs qdnfehdssr vgrgdlrddq drkrdryyer
      781 addrreaske drdeyrkagr kigttedrkr kddyyddrra dqledrredr regfrddyrk
      841 tdrrgdryhd rrdqwkgqes ddewkerrsd rrsdrysrhd yryddrhsdr ysdrgrpssr
      901 tsqdayldey dqrvgdrrvr dnhnrpssrh sesdssspds rrgygtgkhr kyreadrapg
      961 vyydhqksyg taggydynay rrdyynyyhy gyaprsgygy gyydelyrtn paykqqvday
     1021 yarlgyspaq lmerlsvhsg rssangdsrr desfasnted dcdpsildne strldhvpqp
     1081 eevkkfsrph plarfcgang llklvptlsg gnlpppieih slkhlfqkdp qfhelkqfpg
     1141 plvrsqthkn dviqfcthki qsfaedpnlp drsshillwe llvlmlrqng fvsgsdiael
     1201 llrghnilep vvsasglarr ggeggemtsp egaddgssps pdegivvsdr aqpgatgssq
     1261 tlakfrefll fghkkdalew avkcglwgha lslaskmdar tyasimtrft salatndplq
     1321 tlyqhlsgrq paavtciade kwgdwrphla milsnpssrp evdarsittl gdvlasrgcl
     1381 saahfcylma qvefgsysck ssklvllgss hqtllfeefa sneaiqctei yeysqslana
     1441 gymlphlqay kflhatrlae ygfaqealhy cevlaeamgr qcgsaqlaaq tyelasrlky
     1501 hdphfsqgpg eleelgeppw laafaqlvtq llqqhaaaap pqigalygqv sscggnavae
     1561 vsggdsyldq pgftsstgfs mhdsgmsile qsgnygnsyl dpsacgdsaq avspsadqwt
     1621 alahtapdsr sqhlpptsvp sfaatgtsve sgwtavqpsa eysfvstspp vesmvnggqt
     1681 dgtapssppv gsqtttfdyy kastysaqhp phhqtsrerl ssvssstdah drrdsvdsaa
     1741 lasgrsrlps gpqsplttdq sasesertkp aatnkgktqv agkswlggif gkllpkgpnq
     1801 milpddkdpd ivwdehkkcw vdknaapgda diqsaapptd ssfggkptpp sgkpgenrfq
     1861 makqrslrkh yvdvlnpgss kasgdspavp gvppvpeqpg tqsppqflvp qavddhcdsg
     1921 ydfvspapmp dhsapapppp ppetvsyqqp sgkvvstpmm fdpadfssra vdsshkmagg
     1981 nlarrraypt
//
LOCUS       XP_077501102            1990 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X22 [Amblyomma
            americanum].
ACCESSION   XP_077501102
VERSION     XP_077501102.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644976.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1990
                     /product="uncharacterized protein LOC144111913 isoform
                     X22"
                     /calculated_mol_wt=215618
     Region          <349..656
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          619..>853
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1090..>1164
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1138..1502
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1260..1261,1263..1264,1266..1270,1272,1274,1284,
                     1286,1288..1289,1291..1292,1296,1302,1309,1318,1323,1326,
                     1354..1355)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1427,1449..1450,1452..1453,1456..1457,1460,
                     1489..1490,1493,1497)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1990
                     /gene="LOC144111913"
                     /coded_by="XM_077644976.1:158..6130"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqegwp savtgaangs tagsavhcgg paqgdnywnw aahqepgagg dtaasapmqs
      121 wnswgaaqev wtgraqppdp atvggtneaa vapvvndgts lppqlgytsp peggqqqygs
      181 ggvvaegalq egwaaphwsg dtnqwpsnle qqqfvtaqaq wqwqpadass avgqwqgygs
      241 qldpaaagpy estslagyap ldgslqqqqq qpgqdqlaas sgenvagdmh lvayssgpaa
      301 vdgqggsegt qhleatlgdn gmvsmffqdg gaddgqaesr eggptthdqh aeepgppshq
      361 qqtaatgiaq daisqvapaa ssdesvnqev lepvmrpepq dvlplqaegs ppageeavlv
      421 mpegqcpapa kkavirarqg gafkppalar qgssstetal mqqnaiqlhl ppenlellpp
      481 devgrhhasl rhrqgvaspa avgagltlld apemqpcvtl apaappiphd rpasrastgp
      541 qvapeatgce eeesvpdghc qtpvtrmvap plmrqsplgr eegtgrstaa papslrvatv
      601 lapsplladt tlpertepsg stdgdqpqpv vtaaragedk eglpvprpka tstptghcsa
      661 hheppnvsvv lahgkhdskd lgdkgsesht rqeepregkq ddrnsrdfrd srdfresrdp
      721 vhsrdyrdsr dagqargrgs dgesldwkgs qdnfehdssr vgrgdlrddq drkrdryyer
      781 addrreaske drdeyrkagr kigttedrkr kddyyddrra dqledrredr regfrddyrk
      841 tdrrgdryhd rrdqwkgqes ddewkerrsd rrsdrysrhd yryddrhsdr ysdrgrpssr
      901 tsqdayldey dqrvgdrrvr dnhnrpssrh sesdssspds rrgygtgkhr kyreadrapg
      961 vyydhqksyg taggydynay rrdyynyyhy gyaprsgygy gyydelyrtn paykqqvday
     1021 yarlgyspaq lmerlsvhsg rssangdsrr desfasnted dcdpsildne strldhvpqp
     1081 eevkkfsrph plarfcgang llklvptlsg gnlpppieih slkhlfqkdp qfhelkqfpg
     1141 plvrsqthkn dviqfcthki qsfaedpnlp drsshillwe llvlmlrqng fvsgsdiael
     1201 llrghnilep vvsasglarr ggeggemtsp egaddgssps pdegivvsdr aqpgatgssq
     1261 tlakfrefll fghkkdalew avkcglwgha lslaskmdar tyasimtrft salatndplq
     1321 tlyqhlsgrq paavtciade kwgdwrphla milsnpssrp evdarsittl gdvlasrgcl
     1381 saahfcylma qvefgsysck ssklvllgss hqtllfeefa sneaiqctei yeysqslana
     1441 gymlphlqay kflhatrlae ygfaqealhy cevlaeamgr qcgsaqlaaq tyelasrlky
     1501 hdphfsqgpg eleelgeppw laafaqlvtq llqqhaaaap pqigalygqv sscggnavae
     1561 vsggdsyldq pgftsstgfs mhdsgmsile qsgnygnsyl dpsacgdsaq avspsadqwt
     1621 alahtapdsr sqhlpptsvp sfaatgtsve sgwtavqpsa eysfvstspp vesmvnggqt
     1681 dgtapssppv gsqtttfdyy kastysaqhp phhqtsrerl ssvssstdah drrdsvdsaa
     1741 lasgrsrlps gpqsplttdq sasesertkp aatnkgktqv agkswlggif gkllpkgpnq
     1801 milpddkdpd ivwdehkkcw vdknaapgda diqsaapptd ssfggkptpp sgkpgenrfq
     1861 makqrslrkh yvdvlnpgss kasgdspavp gvppvpeqpg tqsppqflvp qavddhcdsg
     1921 ydfvspapmp dhsapapppp ppetvsyqqp sgkvvstpmm fdpadfssra vdsshkmagg
     1981 nlarrraypt
//
LOCUS       XP_077501103            1990 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X23 [Amblyomma
            americanum].
ACCESSION   XP_077501103
VERSION     XP_077501103.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644977.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1990
                     /product="uncharacterized protein LOC144111913 isoform
                     X23"
                     /calculated_mol_wt=215618
     Region          <348..655
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          618..>852
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1089..>1163
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1137..1501
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1259..1260,1262..1263,1265..1269,1271,1273,1283,
                     1285,1287..1288,1290..1291,1295,1301,1308,1317,1322,1325,
                     1353..1354)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1426,1448..1449,1451..1452,1455..1456,1459,
                     1488..1489,1492,1496)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1990
                     /gene="LOC144111913"
                     /coded_by="XM_077644977.1:93..6065"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqegwp savtgaangs tagsavhcgg paqgdnywnw aahqepgagg dtaasapmqs
      121 wnswgaaqev wtgraqppdp atvggtneaa vapvvndgts lppqlgytsp peggqqqygs
      181 ggvvaegalq egwaaphwsg dtnqwpsnle qqqfvtaqaq wwqpadassa vgqwqgygsq
      241 ldpaaagpye stslagyapl dgslqqqqqq pgqdqlaass genvagdmhl vayssgpaav
      301 dgqggsegtq hleatlgdng mvsmffqdgg addgqaesre ggptthdqha eepgppshqq
      361 qtaatgiaqd aisqvapaas sdesvnqevl epvmrpepqd vlplqaegsp pageeavlvm
      421 pegqcpapak kavirarqgg afkppalarq gssstetalm qqnaiqlhlp penlellppd
      481 evgrhhaslr hrqgvaspaa vgagltllda pemqpcvtla paappiphdr pasrastgpq
      541 vapeatgcee eesvpdghcq tpvtrmvapp lmrqsplgre egtgrstaap apslrvatvl
      601 apsplladtt lpertepsgs tdgdqpqpvv taaragedke glpvprpkat stptghcsah
      661 heppnvsvvl ahgkhdskdl gdkgseshtr qeepregkqd drnsrdfrds rdfresrdpv
      721 hsrdyrdsrd agqargrgsd gesldwkgsq dnfehdssrv grgdlrddqd rkrdryyera
      781 ddrreasked rdeyrkagrk igttedrkrk ddyyddrrad qledrredrr egfrddyrkt
      841 drrgdryhdr rdqwkgqesd dewkerrsdr rsdrysrhdy ryddrhsdry sdrgrpssrt
      901 sqdayldeyd qrvgdrrvrd nhnrpssrhs esdssspdsr rgygtgkhrk yreadrapgv
      961 yydhqksygt aggydynayr rdyynyyhyg yaprsgygyg yydelyrtnp aykqqvdayy
     1021 arlgyspaql merlsvhsgr ssangdsrrd esfasntedd cdpsildnes trldhvpqpe
     1081 evkkfsrphp larfcgangl lklvptlsgg nlpppieihs lkhlfqkdpq fhelkqfpgp
     1141 lvrsqthknd viqfcthkiq sfaedpnlpd rsshillwel lvlmlrqngf vsgsdiaell
     1201 lrghnilepv vsasglarrg geggemtspe gaddgsspsp degivvsdra qpgatgssqt
     1261 lakfrefllf ghkkdalewa vkcglwghal slaskmdart yasimtrfts alatndplqt
     1321 lyqhlsgrqp aavtciadek wgdwrphlam ilsnpssrpe vdarsittlg dvlasrgcls
     1381 aahfcylmaq vefgsyscks sklvllgssh qtllfeefas neaiqcteiy eysqslanag
     1441 ymlphlqayk flhatrlaey gfaqealhyc evlaeamgrq cgsaqlaaqt yelasrlkyh
     1501 dphfsqgpge leelgeppwl aafaqlvtql lqqhaaaapp qigalygqvs scggnavaev
     1561 sggdsyldqp gftsstgfsm hdsgmsileq sgnygnsyld psacgdsaqa vspsadqwta
     1621 lahtapdsrs qhlpptsvps faatgtsves gwtavqpsae ysfvstsppv esmvnggqtd
     1681 gtapssppvg sqtttfdyyk astysqaqhp phhqtsrerl ssvssstdah drrdsvdsaa
     1741 lasgrsrlps gpqsplttdq sasesertkp aatnkgktqv agkswlggif gkllpkgpnq
     1801 milpddkdpd ivwdehkkcw vdknaapgda diqsaapptd ssfggkptpp sgkpgenrfq
     1861 makqrslrkh yvdvlnpgss kasgdspavp gvppvpeqpg tqsppqflvp qavddhcdsg
     1921 ydfvspapmp dhsapapppp ppetvsyqqp sgkvvstpmm fdpadfssra vdsshkmagg
     1981 nlarrraypt
//
LOCUS       XP_077501104            1989 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X24 [Amblyomma
            americanum].
ACCESSION   XP_077501104
VERSION     XP_077501104.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644978.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1989
                     /product="uncharacterized protein LOC144111913 isoform
                     X24"
                     /calculated_mol_wt=215490
     Region          <349..656
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          619..>853
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1090..>1164
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1138..1502
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1260..1261,1263..1264,1266..1270,1272,1274,1284,
                     1286,1288..1289,1291..1292,1296,1302,1309,1318,1323,1326,
                     1354..1355)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1427,1449..1450,1452..1453,1456..1457,1460,
                     1489..1490,1493,1497)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1989
                     /gene="LOC144111913"
                     /coded_by="XM_077644978.1:152..6121"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqegwp savtgaangs tagsavhcgg paqgdnywnw aahqepgagg dtaasapmqs
      121 wnswgaaqev wtgraqppdp atvggtneaa vapvvndgts lppqlgytsp peggqqqygs
      181 ggvvaegalq egwaaphwsg dtnqwpsnle qqqfvtaqaq wqwqpadass avgqwqgygs
      241 qldpaaagpy estslagyap ldgslqqqqq qpgqdqlaas sgenvagdmh lvayssgpaa
      301 vdgqggsegt qhleatlgdn gmvsmffqdg gaddgqaesr eggptthdqh aeepgppshq
      361 qqtaatgiaq daisqvapaa ssdesvnqev lepvmrpepq dvlplqaegs ppageeavlv
      421 mpegqcpapa kkavirarqg gafkppalar qgssstetal mqqnaiqlhl ppenlellpp
      481 devgrhhasl rhrqgvaspa avgagltlld apemqpcvtl apaappiphd rpasrastgp
      541 qvapeatgce eeesvpdghc qtpvtrmvap plmrqsplgr eegtgrstaa papslrvatv
      601 lapsplladt tlpertepsg stdgdqpqpv vtaaragedk eglpvprpka tstptghcsa
      661 hheppnvsvv lahgkhdskd lgdkgsesht rqeepregkq ddrnsrdfrd srdfresrdp
      721 vhsrdyrdsr dagqargrgs dgesldwkgs qdnfehdssr vgrgdlrddq drkrdryyer
      781 addrreaske drdeyrkagr kigttedrkr kddyyddrra dqledrredr regfrddyrk
      841 tdrrgdryhd rrdqwkgqes ddewkerrsd rrsdrysrhd yryddrhsdr ysdrgrpssr
      901 tsqdayldey dqrvgdrrvr dnhnrpssrh sesdssspds rrgygtgkhr kyreadrapg
      961 vyydhqksyg taggydynay rrdyynyyhy gyaprsgygy gyydelyrtn paykqqvday
     1021 yarlgyspaq lmerlsvhsg rssangdsrr desfasnted dcdpsildne strldhvpqp
     1081 eevkkfsrph plarfcgang llklvptlsg gnlpppieih slkhlfqkdp qfhelkqfpg
     1141 plvrsqthkn dviqfcthki qsfaedpnlp drsshillwe llvlmlrqng fvsgsdiael
     1201 llrghnilep vvsasglarr ggeggemtsp egaddgssps pdegivvsdr aqpgatgssq
     1261 tlakfrefll fghkkdalew avkcglwgha lslaskmdar tyasimtrft salatndplq
     1321 tlyqhlsgrq paavtciade kwgdwrphla milsnpssrp evdarsittl gdvlasrgcl
     1381 saahfcylma qvefgsysck ssklvllgss hqtllfeefa sneaiqctei yeysqslana
     1441 gymlphlqay kflhatrlae ygfaqealhy cevlaeamgr qcgsaqlaaq tyelasrlky
     1501 hdphfsqgpg eleelgeppw laafaqlvtq llqqhaaaap pqigalygqv sscggnavae
     1561 vsggdsyldq pgftsstgfs mhdsgmsile qsgnygnsyl dpsacgdsaq avspsadqwt
     1621 alahtapdsr sqhlpptsvp sfaatgtsve sgwtavqpsa eysfvstspp vesmvnggqt
     1681 dgtapssppv gsqtttfdyy kastysaqhp phhqtsrerl ssvssstdah drrdsvdsaa
     1741 lasgrsrlps gpqsplttdq sasesertkp aatnkgktqv agkswlggif gkllpkgpnq
     1801 milpddkdpd ivwdehkkcw vdknaapgda diqsaapptd ssfggkptpp sgkpgenrfq
     1861 makqrslrkh yvdvlnpgss kasgdspavp gvppvpeqpg tqsppqflvp qavddhcdsg
     1921 ydfvspapmp dhsapapppp ppetvsyqps gkvvstpmmf dpadfssrav dsshkmaggn
     1981 larrraypt
//
LOCUS       XP_077501105            1988 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X25 [Amblyomma
            americanum].
ACCESSION   XP_077501105
VERSION     XP_077501105.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644979.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1988
                     /product="uncharacterized protein LOC144111913 isoform
                     X25"
                     /calculated_mol_wt=215130
     Region          <346..653
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          616..>850
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1087..>1161
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1135..1499
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1257..1258,1260..1261,1263..1267,1269,1271,1281,
                     1283,1285..1286,1288..1289,1293,1299,1306,1315,1320,1323,
                     1351..1352)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1424,1446..1447,1449..1450,1453..1454,1457,
                     1486..1487,1490,1494)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1988
                     /gene="LOC144111913"
                     /coded_by="XM_077644979.1:28..5994"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 mdcwpvgatg kaaeaaqpak tlpqpvgevm pqwdgstsfq ptqypaalld pqqqqrqqqs
       61 nlqegwpsav tgaangstag savhcggpaq gdnywnwaah qepgaggdta asapmqswns
      121 wgaaqevwtg raqppdpatv ggtneaavap vvndgtslpp qlgytsppeg gqqqygsggv
      181 vaegalqegw aaphwsgdtn qwpsnleqqq fvtaqaqwqw qpadassavg qwqgygsqld
      241 paaagpyest slagyapldg slqqqqqqpg qdqlaassge nvagdmhlva yssgpaavdg
      301 qggsegtqhl eatlgdngmv smffqdggad dgqaesregg ptthdqhaee pgppshqqqt
      361 aatgiaqdai sqvapaassd esvnqevlep vmrpepqdvl plqaegsppa geeavlvmpe
      421 gqcpapakka virarqggaf kppalarqgs sstetalmqq naiqlhlppe nlellppdev
      481 grhhaslrhr qgvaspaavg agltlldape mqpcvtlapa appiphdrpa srastgpqva
      541 peatgceeee svpdghcqtp vtrmvapplm rqsplgreeg tgrstaapap slrvatvlap
      601 splladttlp ertepsgstd gdqpqpvvta aragedkegl pvprpkatst ptghcsahhe
      661 ppnvsvvlah gkhdskdlgd kgseshtrqe epregkqddr nsrdfrdsrd fresrdpvhs
      721 rdyrdsrdag qargrgsdge sldwkgsqdn fehdssrvgr gdlrddqdrk rdryyeradd
      781 rreaskedrd eyrkagrkig ttedrkrkdd yyddrradql edrredrreg frddyrktdr
      841 rgdryhdrrd qwkgqesdde wkerrsdrrs drysrhdyry ddrhsdrysd rgrpssrtsq
      901 dayldeydqr vgdrrvrdnh nrpssrhses dssspdsrrg ygtgkhrkyr eadrapgvyy
      961 dhqksygtag gydynayrrd yynyyhygya prsgygygyy delyrtnpay kqqvdayyar
     1021 lgyspaqlme rlsvhsgrss angdsrrdes fasnteddcd psildnestr ldhvpqpeev
     1081 kkfsrphpla rfcgangllk lvptlsggnl pppieihslk hlfqkdpqfh elkqfpgplv
     1141 rsqthkndvi qfcthkiqsf aedpnlpdrs shillwellv lmlrqngfvs gsdiaelllr
     1201 ghnilepvvs asglarrgge ggemtspega ddgsspspde givvsdraqp gatgssqtla
     1261 kfrefllfgh kkdalewavk cglwghalsl askmdartya simtrftsal atndplqtly
     1321 qhlsgrqpaa vtciadekwg dwrphlamil snpssrpevd arsittlgdv lasrgclsaa
     1381 hfcylmaqve fgsysckssk lvllgsshqt llfeefasne aiqcteiyey sqslanagym
     1441 lphlqaykfl hatrlaeygf aqealhycev laeamgrqcg saqlaaqtye lasrlkyhdp
     1501 hfsqgpgele elgeppwlaa faqlvtqllq qhaaaappqi galygqvssc ggnavaevsg
     1561 gdsyldqpgf tsstgfsmhd sgmsileqsg nygnsyldps acgdsaqavs psadqwtala
     1621 htapdsrsqh lpptsvpsfa atgtsvesgw tavqpsaeys fvstsppves mvnggqtdgt
     1681 apssppvgsq tttfdyykas tysqaqhpph hqtsrerlss vssstdahdr rdsvdsaala
     1741 sgrsrlpsgp qsplttdqsa sesertkpaa tnkgktqvag kswlggifgk llpkgpnqmi
     1801 lpddkdpdiv wdehkkcwvd knaapgdadi qsaapptdss fggkptppsg kpgenrfqma
     1861 kqrslrkhyv dvlnpgsska sgdspavpgv ppvpeqpgtq sppqflvpqa vddhcdsgyd
     1921 fvspapmpdh sapapppppp etvsyqqpsg kvvstpmmfd padfssravd sshkmaggnl
     1981 arrraypt
//
LOCUS       XP_077501106            1987 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X26 [Amblyomma
            americanum].
ACCESSION   XP_077501106
VERSION     XP_077501106.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644980.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1987
                     /product="uncharacterized protein LOC144111913 isoform
                     X26"
                     /calculated_mol_wt=215002
     Region          <346..653
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          616..>850
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1087..>1161
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1135..1499
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1257..1258,1260..1261,1263..1267,1269,1271,1281,
                     1283,1285..1286,1288..1289,1293,1299,1306,1315,1320,1323,
                     1351..1352)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1424,1446..1447,1449..1450,1453..1454,1457,
                     1486..1487,1490,1494)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1987
                     /gene="LOC144111913"
                     /coded_by="XM_077644980.1:28..5991"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 mdcwpvgatg kaaeaaqpak tlpqpvgevm pqwdgstsfq ptqypaalld pqqqqrqqqs
       61 nlqegwpsav tgaangstag savhcggpaq gdnywnwaah qepgaggdta asapmqswns
      121 wgaaqevwtg raqppdpatv ggtneaavap vvndgtslpp qlgytsppeg gqqqygsggv
      181 vaegalqegw aaphwsgdtn qwpsnleqqq fvtaqaqwqw qpadassavg qwqgygsqld
      241 paaagpyest slagyapldg slqqqqqqpg qdqlaassge nvagdmhlva yssgpaavdg
      301 qggsegtqhl eatlgdngmv smffqdggad dgqaesregg ptthdqhaee pgppshqqqt
      361 aatgiaqdai sqvapaassd esvnqevlep vmrpepqdvl plqaegsppa geeavlvmpe
      421 gqcpapakka virarqggaf kppalarqgs sstetalmqq naiqlhlppe nlellppdev
      481 grhhaslrhr qgvaspaavg agltlldape mqpcvtlapa appiphdrpa srastgpqva
      541 peatgceeee svpdghcqtp vtrmvapplm rqsplgreeg tgrstaapap slrvatvlap
      601 splladttlp ertepsgstd gdqpqpvvta aragedkegl pvprpkatst ptghcsahhe
      661 ppnvsvvlah gkhdskdlgd kgseshtrqe epregkqddr nsrdfrdsrd fresrdpvhs
      721 rdyrdsrdag qargrgsdge sldwkgsqdn fehdssrvgr gdlrddqdrk rdryyeradd
      781 rreaskedrd eyrkagrkig ttedrkrkdd yyddrradql edrredrreg frddyrktdr
      841 rgdryhdrrd qwkgqesdde wkerrsdrrs drysrhdyry ddrhsdrysd rgrpssrtsq
      901 dayldeydqr vgdrrvrdnh nrpssrhses dssspdsrrg ygtgkhrkyr eadrapgvyy
      961 dhqksygtag gydynayrrd yynyyhygya prsgygygyy delyrtnpay kqqvdayyar
     1021 lgyspaqlme rlsvhsgrss angdsrrdes fasnteddcd psildnestr ldhvpqpeev
     1081 kkfsrphpla rfcgangllk lvptlsggnl pppieihslk hlfqkdpqfh elkqfpgplv
     1141 rsqthkndvi qfcthkiqsf aedpnlpdrs shillwellv lmlrqngfvs gsdiaelllr
     1201 ghnilepvvs asglarrgge ggemtspega ddgsspspde givvsdraqp gatgssqtla
     1261 kfrefllfgh kkdalewavk cglwghalsl askmdartya simtrftsal atndplqtly
     1321 qhlsgrqpaa vtciadekwg dwrphlamil snpssrpevd arsittlgdv lasrgclsaa
     1381 hfcylmaqve fgsysckssk lvllgsshqt llfeefasne aiqcteiyey sqslanagym
     1441 lphlqaykfl hatrlaeygf aqealhycev laeamgrqcg saqlaaqtye lasrlkyhdp
     1501 hfsqgpgele elgeppwlaa faqlvtqllq qhaaaappqi galygqvssc ggnavaevsg
     1561 gdsyldqpgf tsstgfsmhd sgmsileqsg nygnsyldps acgdsaqavs psadqwtala
     1621 htapdsrsqh lpptsvpsfa atgtsvesgw tavqpsaeys fvstsppves mvnggqtdgt
     1681 apssppvgsq tttfdyykas tysaqhpphh qtsrerlssv ssstdahdrr dsvdsaalas
     1741 grsrlpsgpq splttdqsas esertkpaat nkgktqvagk swlggifgkl lpkgpnqmil
     1801 pddkdpdivw dehkkcwvdk naapgdadiq saapptdssf ggkptppsgk pgenrfqmak
     1861 qrslrkhyvd vlnpgsskas gdspavpgvp pvpeqpgtqs ppqflvpqav ddhcdsgydf
     1921 vspapmpdhs apappppppe tvsyqqpsgk vvstpmmfdp adfssravds shkmaggnla
     1981 rrraypt
//
LOCUS       XP_077501107            1987 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X27 [Amblyomma
            americanum].
ACCESSION   XP_077501107
VERSION     XP_077501107.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644981.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1987
                     /product="uncharacterized protein LOC144111913 isoform
                     X27"
                     /calculated_mol_wt=215002
     Region          <345..652
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          615..>849
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1086..>1160
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1134..1498
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1256..1257,1259..1260,1262..1266,1268,1270,1280,
                     1282,1284..1285,1287..1288,1292,1298,1305,1314,1319,1322,
                     1350..1351)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1423,1445..1446,1448..1449,1452..1453,1456,
                     1485..1486,1489,1493)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1987
                     /gene="LOC144111913"
                     /coded_by="XM_077644981.1:28..5991"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 mdcwpvgatg kaaeaaqpak tlpqpvgevm pqwdgstsfq ptqypaalld pqqqqrqqqs
       61 nlqegwpsav tgaangstag savhcggpaq gdnywnwaah qepgaggdta asapmqswns
      121 wgaaqevwtg raqppdpatv ggtneaavap vvndgtslpp qlgytsppeg gqqqygsggv
      181 vaegalqegw aaphwsgdtn qwpsnleqqq fvtaqaqwwq padassavgq wqgygsqldp
      241 aaagpyests lagyapldgs lqqqqqqpgq dqlaassgen vagdmhlvay ssgpaavdgq
      301 ggsegtqhle atlgdngmvs mffqdggadd gqaesreggp tthdqhaeep gppshqqqta
      361 atgiaqdais qvapaassde svnqevlepv mrpepqdvlp lqaegsppag eeavlvmpeg
      421 qcpapakkav irarqggafk ppalarqgss stetalmqqn aiqlhlppen lellppdevg
      481 rhhaslrhrq gvaspaavga gltlldapem qpcvtlapaa ppiphdrpas rastgpqvap
      541 eatgceeees vpdghcqtpv trmvapplmr qsplgreegt grstaapaps lrvatvlaps
      601 plladttlpe rtepsgstdg dqpqpvvtaa ragedkeglp vprpkatstp tghcsahhep
      661 pnvsvvlahg khdskdlgdk gseshtrqee pregkqddrn srdfrdsrdf resrdpvhsr
      721 dyrdsrdagq argrgsdges ldwkgsqdnf ehdssrvgrg dlrddqdrkr dryyeraddr
      781 reaskedrde yrkagrkigt tedrkrkddy yddrradqle drredrregf rddyrktdrr
      841 gdryhdrrdq wkgqesddew kerrsdrrsd rysrhdyryd drhsdrysdr grpssrtsqd
      901 ayldeydqrv gdrrvrdnhn rpssrhsesd ssspdsrrgy gtgkhrkyre adrapgvyyd
      961 hqksygtagg ydynayrrdy ynyyhygyap rsgygygyyd elyrtnpayk qqvdayyarl
     1021 gyspaqlmer lsvhsgrssa ngdsrrdesf asnteddcdp sildnestrl dhvpqpeevk
     1081 kfsrphplar fcgangllkl vptlsggnlp ppieihslkh lfqkdpqfhe lkqfpgplvr
     1141 sqthkndviq fcthkiqsfa edpnlpdrss hillwellvl mlrqngfvsg sdiaelllrg
     1201 hnilepvvsa sglarrggeg gemtspegad dgsspspdeg ivvsdraqpg atgssqtlak
     1261 frefllfghk kdalewavkc glwghalsla skmdartyas imtrftsala tndplqtlyq
     1321 hlsgrqpaav tciadekwgd wrphlamils npssrpevda rsittlgdvl asrgclsaah
     1381 fcylmaqvef gsyscksskl vllgsshqtl lfeefasnea iqcteiyeys qslanagyml
     1441 phlqaykflh atrlaeygfa qealhycevl aeamgrqcgs aqlaaqtyel asrlkyhdph
     1501 fsqgpgelee lgeppwlaaf aqlvtqllqq haaaappqig alygqvsscg gnavaevsgg
     1561 dsyldqpgft sstgfsmhds gmsileqsgn ygnsyldpsa cgdsaqavsp sadqwtalah
     1621 tapdsrsqhl pptsvpsfaa tgtsvesgwt avqpsaeysf vstsppvesm vnggqtdgta
     1681 pssppvgsqt ttfdyykast ysqaqhpphh qtsrerlssv ssstdahdrr dsvdsaalas
     1741 grsrlpsgpq splttdqsas esertkpaat nkgktqvagk swlggifgkl lpkgpnqmil
     1801 pddkdpdivw dehkkcwvdk naapgdadiq saapptdssf ggkptppsgk pgenrfqmak
     1861 qrslrkhyvd vlnpgsskas gdspavpgvp pvpeqpgtqs ppqflvpqav ddhcdsgydf
     1921 vspapmpdhs apappppppe tvsyqqpsgk vvstpmmfdp adfssravds shkmaggnla
     1981 rrraypt
//
LOCUS       XP_077501108            1985 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X28 [Amblyomma
            americanum].
ACCESSION   XP_077501108
VERSION     XP_077501108.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644982.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1985
                     /product="uncharacterized protein LOC144111913 isoform
                     X28"
                     /calculated_mol_wt=215067
     Region          <349..651
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          614..>848
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1085..>1159
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1133..1497
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1255..1256,1258..1259,1261..1265,1267,1269,1279,
                     1281,1283..1284,1286..1287,1291,1297,1304,1313,1318,1321,
                     1349..1350)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1422,1444..1445,1447..1448,1451..1452,1455,
                     1484..1485,1488,1492)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1985
                     /gene="LOC144111913"
                     /coded_by="XM_077644982.1:93..6050"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqegwp savtgaangs tagsavhcgg paqgdnywnw aahqepgagg dtaasapmqs
      121 wnswgaaqev wtgraqppdp atvggtneaa vapvvndgts lppqlgytsp peggqqqygs
      181 ggvvaegalq egwaaphwsg dtnqwpsnle qqqfvtaqaq wqwqpadass avgqwqgygs
      241 qldpaaagpy estslagyap ldgslqqqqq qpgqdqlaas sgenvagdmh lvayssgpaa
      301 vdgqggsegt qhleatlgdn gmvsmffqdg gaddgqaesr eggptthdqh aeepgppshq
      361 qqtaatgiaq daisqvapaa ssdesvnqev lepvmrpepq daegsppage eavlvmpegq
      421 cpapakkavi rarqggafkp palarqgsss tetalmqqna iqlhlppenl ellppdevgr
      481 hhaslrhrqg vaspaavgag ltlldapemq pcvtlapaap piphdrpasr astgpqvape
      541 atgceeeesv pdghcqtpvt rmvapplmrq splgreegtg rstaapapsl rvatvlapsp
      601 lladttlper tepsgstdgd qpqpvvtaar agedkeglpv prpkatstpt ghcsahhepp
      661 nvsvvlahgk hdskdlgdkg seshtrqeep regkqddrns rdfrdsrdfr esrdpvhsrd
      721 yrdsrdagqa rgrgsdgesl dwkgsqdnfe hdssrvgrgd lrddqdrkrd ryyeraddrr
      781 easkedrdey rkagrkigtt edrkrkddyy ddrradqled rredrregfr ddyrktdrrg
      841 dryhdrrdqw kgqesddewk errsdrrsdr ysrhdyrydd rhsdrysdrg rpssrtsqda
      901 yldeydqrvg drrvrdnhnr pssrhsesds sspdsrrgyg tgkhrkyrea drapgvyydh
      961 qksygtaggy dynayrrdyy nyyhygyapr sgygygyyde lyrtnpaykq qvdayyarlg
     1021 yspaqlmerl svhsgrssan gdsrrdesfa snteddcdps ildnestrld hvpqpeevkk
     1081 fsrphplarf cgangllklv ptlsggnlpp pieihslkhl fqkdpqfhel kqfpgplvrs
     1141 qthkndviqf cthkiqsfae dpnlpdrssh illwellvlm lrqngfvsgs diaelllrgh
     1201 nilepvvsas glarrggegg emtspegadd gsspspdegi vvsdraqpga tgssqtlakf
     1261 refllfghkk dalewavkcg lwghalslas kmdartyasi mtrftsalat ndplqtlyqh
     1321 lsgrqpaavt ciadekwgdw rphlamilsn pssrpevdar sittlgdvla srgclsaahf
     1381 cylmaqvefg sysckssklv llgsshqtll feefasneai qcteiyeysq slanagymlp
     1441 hlqaykflha trlaeygfaq ealhycevla eamgrqcgsa qlaaqtyela srlkyhdphf
     1501 sqgpgeleel geppwlaafa qlvtqllqqh aaaappqiga lygqvsscgg navaevsggd
     1561 syldqpgfts stgfsmhdsg msileqsgny gnsyldpsac gdsaqavsps adqwtalaht
     1621 apdsrsqhlp ptsvpsfaat gtsvesgwta vqpsaeysfv stsppvesmv nggqtdgtap
     1681 ssppvgsqtt tfdyykasty saqhpphhqt srerlssvss stdahdrrds vdsaalasgr
     1741 srlpsgpqsp lttdqsases ertkpaatnk gktqvagksw lggifgkllp kgpnqmilpd
     1801 dkdpdivwde hkkcwvdkna apgdadiqsa apptdssfgg kptppsgkpg enrfqmakqr
     1861 slrkhyvdvl npgsskasgd spavpgvppv peqpgtqspp qflvpqavdd hcdsgydfvs
     1921 papmpdhsap appppppetv syqqpsgkvv stpmmfdpad fssravdssh kmaggnlarr
     1981 raypt
//
LOCUS       XP_077501109            1983 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X29 [Amblyomma
            americanum].
ACCESSION   XP_077501109
VERSION     XP_077501109.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644983.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1983
                     /product="uncharacterized protein LOC144111913 isoform
                     X29"
                     /calculated_mol_wt=215594
     Region          <327..649
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          612..>846
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1083..>1157
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1131..1495
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1253..1254,1256..1257,1259..1263,1265,1267,1277,
                     1279,1281..1282,1284..1285,1289,1295,1302,1311,1316,1319,
                     1347..1348)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1420,1442..1443,1445..1446,1449..1450,1453,
                     1482..1483,1486,1490)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1983
                     /gene="LOC144111913"
                     /coded_by="XM_077644983.1:93..6044"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqegwp savtgaangs tagsavhcgg paqgdnywnw aahqepgagg dtaasapmqs
      121 wnswgaaqev wtgraqppdp atvggtneaa vapvvndgts lppqlgytsp peggqqqygs
      181 ggvvaegalq egwaaphwsg dtnqwpsnle qqqfvtaqaq wqwqpadass avgqwqgygs
      241 qldpaaagpy estslagyap ldgslqqqqq qpgqdqlaas sgenvagtqh leatlgdngm
      301 vsmffqdgga ddgqaesreg gptthdqhae epgppshqqq taatgiaqda isqvapaass
      361 desvnqevle pvmrpepqdv lplqaegspp ageeavlvmp egqcpapakk avirarqgga
      421 fkppalarqg ssstetdylr viyqllwylw halmqqnaiq lhlppenlel lppdevgrhh
      481 aslrhrqgva spaavgaglt lldapemqpc vtlapaappi phdrpasras tgpqvapeat
      541 gceeeesvpd ghcqtpvtrm vapplmrqsp lgreegtgrs taapapslrv atvlapspll
      601 adttlperte psgstdgdqp qpvvtaarag edkeglpvpr pkatstptgh csahheppnv
      661 svvlahgkhd skdlgdkgse shtrqeepre gkqddrnsrd frdsrdfres rdpvhsrdyr
      721 dsrdagqarg rgsdgesldw kgsqdnfehd ssrvgrgdlr ddqdrkrdry yeraddrrea
      781 skedrdeyrk agrkigtted rkrkddyydd rradqledrr edrregfrdd yrktdrrgdr
      841 yhdrrdqwkg qesddewker rsdrrsdrys rhdyryddrh sdrysdrgrp ssrtsqdayl
      901 deydqrvgdr rvrdnhnrps srhsesdsss pdsrrgygtg khrkyreadr apgvyydhqk
      961 sygtaggydy nayrrdyyny yhygyaprsg ygygyydely rtnpaykqqv dayyarlgys
     1021 paqlmerlsv hsgrssangd srrdesfasn teddcdpsil dnestrldhv pqpeevkkfs
     1081 rphplarfcg angllklvpt lsggnlpppi eihslkhlfq kdpqfhelkq fpgplvrsqt
     1141 hkndviqfct hkiqsfaedp nlpdrsshil lwellvlmlr qngfvsgsdi aelllrghni
     1201 lepvvsasgl arrggeggem tspegaddgs spspdegivv sdraqpgatg ssqtlakfre
     1261 fllfghkkda lewavkcglw ghalslaskm dartyasimt rftsalatnd plqtlyqhls
     1321 grqpaavtci adekwgdwrp hlamilsnps srpevdarsi ttlgdvlasr gclsaahfcy
     1381 lmaqvefgsy sckssklvll gsshqtllfe efasneaiqc teiyeysqsl anagymlphl
     1441 qaykflhatr laeygfaqea lhycevlaea mgrqcgsaql aaqtyelasr lkyhdphfsq
     1501 gpgeleelge ppwlaafaql vtqllqqhaa aappqigaly gqvsscggna vaevsggdsy
     1561 ldqpgftsst gfsmhdsgms ileqsgnygn syldpsacgd saqavspsad qwtalahtap
     1621 dsrsqhlppt svpsfaatgt svesgwtavq psaeysfvst sppvesmvng gqtdgtapss
     1681 ppvgsqtttf dyykastysa qhpphhqtsr erlssvssst dahdrrdsvd saalasgrsr
     1741 lpsgpqsplt tdqsaseser tkpaatnkgk tqvagkswlg gifgkllpkg pnqmilpddk
     1801 dpdivwdehk kcwvdknaap gdadiqsaap ptdssfggkp tppsgkpgen rfqmakqrsl
     1861 rkhyvdvlnp gsskasgdsp avpgvppvpe qpgtqsppqf lvpqavddhc dsgydfvspa
     1921 pmpdhsapap pppppetvsy qqpsgkvvst pmmfdpadfs sravdsshkm aggnlarrra
     1981 ypt
//
LOCUS       XP_077501110            1983 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X30 [Amblyomma
            americanum].
ACCESSION   XP_077501110
VERSION     XP_077501110.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644984.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1983
                     /product="uncharacterized protein LOC144111913 isoform
                     X30"
                     /calculated_mol_wt=215374
     Region          <357..679
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          642..>876
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1113..>1187
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1161..1525
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1283..1284,1286..1287,1289..1293,1295,1297,1307,
                     1309,1311..1312,1314..1315,1319,1325,1332,1341,1346,1349,
                     1377..1378)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1450,1472..1473,1475..1476,1479..1480,1483,
                     1512..1513,1516,1520)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1983
                     /gene="LOC144111913"
                     /coded_by="XM_077644984.1:93..6044"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqtccc lamqegwpsa vtgaangsta gsavhcggpa qgdnywnwaa hqepgaggdt
      121 aasapmqswn swgaaqevwt graqppdpat vggtneaava pvvndgtslp pqlgytsppe
      181 ggqqqygsgg vvaegalqeg waaphwsgdt nqwpsnleqq qfvtaqaqwq wqpadassav
      241 gqwqgygsql dpaaagpyes tslagyapld gslqqqqqqp gqdqlaassg envagdmhlv
      301 ayssgpaavd gqggsegtqh leatlgdngm vsmffqdgga ddgqaesreg gptthdqhae
      361 epgppshqqq taatgiaqda isqvapaass desvnqevle pvmrpepqdv lplqaegspp
      421 ageeavlvmp egqcpapakk avirarqgga fkppalarqg ssstetdylr viyqllwylw
      481 halmqqnaiq lhlppenlel lppdevgrhh aslrhrqgva spaavgaglt lldapemqpc
      541 vtlapaappi phdrpasras tgpqvapeat gceeeesvpd ghcqtpvtrm vapplmrqsp
      601 lgreegtgrs taapapslrv atvlapspll adttlperte psgstdgdqp qpvvtaarag
      661 edkeglpvpr pkatstptgh csahheppnv svvlahgkhd skdlgdkgse shtrqeepre
      721 gkqddrnsrd frdsrdfres rdpvhsrdyr dsrdagqarg rgsdgesldw kgsqdnfehd
      781 ssrvgrgdlr ddqdrkrdry yeraddrrea skedrdeyrk agrkigtted rkrkddyydd
      841 rradqledrr edrregfrdd yrktdrrgdr yhdrrdqwkg qesddewker rsdrrsdrys
      901 rhdyryddrh sdrysdrgrp ssrtsqdayl deydqrvgdr rvrdnhnrps srhsesdsss
      961 pdsrrgygtg khrkyreadr apgvyydhqk sygtaggydy nayrrdyyny yhygyaprsg
     1021 ygygyydely rtnpaykqqv dayyarlgys paqlmerlsv hsgrssangd srrdesfasn
     1081 teddcdpsil dnestrldhv pqpeevkkfs rphplarfcg angllklvpt lsggnlpppi
     1141 eihslkhlfq kdpqfhelkq fpgplvrsqt hkndviqfct hkiqsfaedp nlpdrsshil
     1201 lwellvlmlr qngfvsgsdi aelllrghni lepvvsasgl arrggeggem tspegaddgs
     1261 spspdegivv sdraqpgatg ssqtlakfre fllfghkkda lewavkcglw ghalslaskm
     1321 dartyasimt rftsalatnd plqtlyqhls grqpaavtci adekwgdwrp hlamilsnps
     1381 srpevdarsi ttlgdvlasr gclsaahfcy lmaqvefgsy sckssklvll gsshqtllfe
     1441 efasneaiqc teiyeysqsl anagymlphl qaykflhatr laeygfaqea lhycevlaea
     1501 mgrqcgsaql aaqtyelasr lkyhdphfsq gpgeleelge ppwlaafaql vtqllqqhaa
     1561 aappqigaly gqvsscggna vaevsggdsy ldqpgftsst gfsmhdsgms ileqsgnygn
     1621 syldpsacgd saqavspsad qwtalahtap dsrsqhlppt svpsfaatgt svesgwtavq
     1681 psaeysfvst sppvesmvng gqtdgtapss ppvgsqtttf dyykastysq aqhpphhqts
     1741 rerlssvsss tdahdrrdsv dsaalasgrs rlpsgpqspl ttdqsasese rtkpaatnkg
     1801 ktqivwdehk kcwvdknaap gdadiqsaap ptdssfggkp tppsgkpgen rfqmakqrsl
     1861 rkhyvdvlnp gsskasgdsp avpgvppvpe qpgtqsppqf lvpqavddhc dsgydfvspa
     1921 pmpdhsapap pppppetvsy qqpsgkvvst pmmfdpadfs sravdsshkm aggnlarrra
     1981 ypt
//
LOCUS       XP_077501111            1982 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X31 [Amblyomma
            americanum].
ACCESSION   XP_077501111
VERSION     XP_077501111.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644985.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1982
                     /product="uncharacterized protein LOC144111913 isoform
                     X31"
                     /calculated_mol_wt=215246
     Region          <357..679
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          642..>876
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1113..>1187
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1161..1525
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1283..1284,1286..1287,1289..1293,1295,1297,1307,
                     1309,1311..1312,1314..1315,1319,1325,1332,1341,1346,1349,
                     1377..1378)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1450,1472..1473,1475..1476,1479..1480,1483,
                     1512..1513,1516,1520)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1982
                     /gene="LOC144111913"
                     /coded_by="XM_077644985.1:93..6041"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqtccc lamqegwpsa vtgaangsta gsavhcggpa qgdnywnwaa hqepgaggdt
      121 aasapmqswn swgaaqevwt graqppdpat vggtneaava pvvndgtslp pqlgytsppe
      181 ggqqqygsgg vvaegalqeg waaphwsgdt nqwpsnleqq qfvtaqaqwq wqpadassav
      241 gqwqgygsql dpaaagpyes tslagyapld gslqqqqqqp gqdqlaassg envagdmhlv
      301 ayssgpaavd gqggsegtqh leatlgdngm vsmffqdgga ddgqaesreg gptthdqhae
      361 epgppshqqq taatgiaqda isqvapaass desvnqevle pvmrpepqdv lplqaegspp
      421 ageeavlvmp egqcpapakk avirarqgga fkppalarqg ssstetdylr viyqllwylw
      481 halmqqnaiq lhlppenlel lppdevgrhh aslrhrqgva spaavgaglt lldapemqpc
      541 vtlapaappi phdrpasras tgpqvapeat gceeeesvpd ghcqtpvtrm vapplmrqsp
      601 lgreegtgrs taapapslrv atvlapspll adttlperte psgstdgdqp qpvvtaarag
      661 edkeglpvpr pkatstptgh csahheppnv svvlahgkhd skdlgdkgse shtrqeepre
      721 gkqddrnsrd frdsrdfres rdpvhsrdyr dsrdagqarg rgsdgesldw kgsqdnfehd
      781 ssrvgrgdlr ddqdrkrdry yeraddrrea skedrdeyrk agrkigtted rkrkddyydd
      841 rradqledrr edrregfrdd yrktdrrgdr yhdrrdqwkg qesddewker rsdrrsdrys
      901 rhdyryddrh sdrysdrgrp ssrtsqdayl deydqrvgdr rvrdnhnrps srhsesdsss
      961 pdsrrgygtg khrkyreadr apgvyydhqk sygtaggydy nayrrdyyny yhygyaprsg
     1021 ygygyydely rtnpaykqqv dayyarlgys paqlmerlsv hsgrssangd srrdesfasn
     1081 teddcdpsil dnestrldhv pqpeevkkfs rphplarfcg angllklvpt lsggnlpppi
     1141 eihslkhlfq kdpqfhelkq fpgplvrsqt hkndviqfct hkiqsfaedp nlpdrsshil
     1201 lwellvlmlr qngfvsgsdi aelllrghni lepvvsasgl arrggeggem tspegaddgs
     1261 spspdegivv sdraqpgatg ssqtlakfre fllfghkkda lewavkcglw ghalslaskm
     1321 dartyasimt rftsalatnd plqtlyqhls grqpaavtci adekwgdwrp hlamilsnps
     1381 srpevdarsi ttlgdvlasr gclsaahfcy lmaqvefgsy sckssklvll gsshqtllfe
     1441 efasneaiqc teiyeysqsl anagymlphl qaykflhatr laeygfaqea lhycevlaea
     1501 mgrqcgsaql aaqtyelasr lkyhdphfsq gpgeleelge ppwlaafaql vtqllqqhaa
     1561 aappqigaly gqvsscggna vaevsggdsy ldqpgftsst gfsmhdsgms ileqsgnygn
     1621 syldpsacgd saqavspsad qwtalahtap dsrsqhlppt svpsfaatgt svesgwtavq
     1681 psaeysfvst sppvesmvng gqtdgtapss ppvgsqtttf dyykastysa qhpphhqtsr
     1741 erlssvssst dahdrrdsvd saalasgrsr lpsgpqsplt tdqsaseser tkpaatnkgk
     1801 tqivwdehkk cwvdknaapg dadiqsaapp tdssfggkpt ppsgkpgenr fqmakqrslr
     1861 khyvdvlnpg sskasgdspa vpgvppvpeq pgtqsppqfl vpqavddhcd sgydfvspap
     1921 mpdhsapapp ppppetvsyq qpsgkvvstp mmfdpadfss ravdsshkma ggnlarrray
     1981 pt
//
LOCUS       XP_077501112            1982 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X32 [Amblyomma
            americanum].
ACCESSION   XP_077501112
VERSION     XP_077501112.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644986.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1982
                     /product="uncharacterized protein LOC144111913 isoform
                     X32"
                     /calculated_mol_wt=215142
     Region          <325..647
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          610..>844
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1081..>1155
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1129..1493
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1251..1252,1254..1255,1257..1261,1263,1265,1275,
                     1277,1279..1280,1282..1283,1287,1293,1300,1309,1314,1317,
                     1345..1346)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1418,1440..1441,1443..1444,1447..1448,1451,
                     1480..1481,1484,1488)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1982
                     /gene="LOC144111913"
                     /coded_by="XM_077644986.1:88..6036"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 mpqwdgstsf qptqypaall dpqqqqrqqq snlqtcccla mqegwpsavt gaangstags
       61 avhcggpaqg dnywnwaahq epgaggdtaa sapmqswnsw gaaqevwtgr aqppdpatvg
      121 gtneaavapv vndgtslppq lgytsppegg qqqygsggvv aegalqegwa aphwsgdtnq
      181 wpsnleqqqf vtaqaqwqwq padassavgq wqgygsqldp aaagpyests lagyapldgs
      241 lqqqqqqpgq dqlaassgen vagdmhlvay ssgpaavdgq ggsegtqhle atlgdngmvs
      301 mffqdggadd gqaesreggp tthdqhaeep gppshqqqta atgiaqdais qvapaassde
      361 svnqevlepv mrpepqdvlp lqaegsppag eeavlvmpeg qcpapakkav irarqggafk
      421 ppalarqgss stetdylrvi yqllwylwha lmqqnaiqlh lppenlellp pdevgrhhas
      481 lrhrqgvasp aavgagltll dapemqpcvt lapaappiph drpasrastg pqvapeatgc
      541 eeeesvpdgh cqtpvtrmva pplmrqsplg reegtgrsta apapslrvat vlapspllad
      601 ttlperteps gstdgdqpqp vvtaaraged keglpvprpk atstptghcs ahheppnvsv
      661 vlahgkhdsk dlgdkgsesh trqeepregk qddrnsrdfr dsrdfresrd pvhsrdyrds
      721 rdagqargrg sdgesldwkg sqdnfehdss rvgrgdlrdd qdrkrdryye raddrreask
      781 edrdeyrkag rkigttedrk rkddyyddrr adqledrred rregfrddyr ktdrrgdryh
      841 drrdqwkgqe sddewkerrs drrsdrysrh dyryddrhsd rysdrgrpss rtsqdaylde
      901 ydqrvgdrrv rdnhnrpssr hsesdssspd srrgygtgkh rkyreadrap gvyydhqksy
      961 gtaggydyna yrrdyynyyh ygyaprsgyg ygyydelyrt npaykqqvda yyarlgyspa
     1021 qlmerlsvhs grssangdsr rdesfasnte ddcdpsildn estrldhvpq peevkkfsrp
     1081 hplarfcgan gllklvptls ggnlpppiei hslkhlfqkd pqfhelkqfp gplvrsqthk
     1141 ndviqfcthk iqsfaedpnl pdrsshillw ellvlmlrqn gfvsgsdiae lllrghnile
     1201 pvvsasglar rggeggemts pegaddgssp spdegivvsd raqpgatgss qtlakfrefl
     1261 lfghkkdale wavkcglwgh alslaskmda rtyasimtrf tsalatndpl qtlyqhlsgr
     1321 qpaavtciad ekwgdwrphl amilsnpssr pevdarsitt lgdvlasrgc lsaahfcylm
     1381 aqvefgsysc kssklvllgs shqtllfeef asneaiqcte iyeysqslan agymlphlqa
     1441 ykflhatrla eygfaqealh ycevlaeamg rqcgsaqlaa qtyelasrlk yhdphfsqgp
     1501 geleelgepp wlaafaqlvt qllqqhaaaa ppqigalygq vsscggnava evsggdsyld
     1561 qpgftsstgf smhdsgmsil eqsgnygnsy ldpsacgdsa qavspsadqw talahtapds
     1621 rsqhlpptsv psfaatgtsv esgwtavqps aeysfvstsp pvesmvnggq tdgtapsspp
     1681 vgsqtttfdy ykastysqaq hpphhqtsre rlssvssstd ahdrrdsvds aalasgrsrl
     1741 psgpqspltt dqsasesert kpaatnkgkt qvagkswlgg ifgkllpkgp nqmilpddkd
     1801 pdivwdehkk cwvdknaapg dadiqsaapp tdssfggkpt ppsgkpgenr fqmakqrslr
     1861 khyvdvlnpg sskasgdspa vpgvppvpeq pgtqsppqfl vpqavddhcd sgydfvspap
     1921 mpdhsapapp ppppetvsyq qpsgkvvstp mmfdpadfss ravdsshkma ggnlarrray
     1981 pt
//
LOCUS       XP_077501113            1980 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X33 [Amblyomma
            americanum].
ACCESSION   XP_077501113
VERSION     XP_077501113.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644987.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1980
                     /product="uncharacterized protein LOC144111913 isoform
                     X33"
                     /calculated_mol_wt=214978
     Region          <324..646
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          609..>843
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1080..>1154
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1128..1492
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1250..1251,1253..1254,1256..1260,1262,1264,1274,
                     1276,1278..1279,1281..1282,1286,1292,1299,1308,1313,1316,
                     1344..1345)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1417,1439..1440,1442..1443,1446..1447,1450,
                     1479..1480,1483,1487)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1980
                     /gene="LOC144111913"
                     /coded_by="XM_077644987.1:21..5963"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 mdcwpvgatg kaaeaaqpak tlpqpvgevm pqwdgstsfq ptqypaalld pqqqqrqqqs
       61 nlqegwpsav tgaangstag savhcggpaq gdnywnwaah qepgaggdta asapmqswns
      121 wgaaqevwtg raqppdpatv ggtneaavap vvndgtslpp qlgytsppeg gqqqygsggv
      181 vaegalqegw aaphwsgdtn qwpsnleqqq fvtaqaqwqw qpadassavg qwqgygsqld
      241 paaagpyest slagyapldg slqqqqqqpg qdqlaassge nvagtqhlea tlgdngmvsm
      301 ffqdggaddg qaesreggpt thdqhaeepg ppshqqqtaa tgiaqdaisq vapaassdes
      361 vnqevlepvm rpepqdvlpl qaegsppage eavlvmpegq cpapakkavi rarqggafkp
      421 palarqgsss tetdylrviy qllwylwhal mqqnaiqlhl ppenlellpp devgrhhasl
      481 rhrqgvaspa avgagltlld apemqpcvtl apaappiphd rpasrastgp qvapeatgce
      541 eeesvpdghc qtpvtrmvap plmrqsplgr eegtgrstaa papslrvatv lapsplladt
      601 tlpertepsg stdgdqpqpv vtaaragedk eglpvprpka tstptghcsa hheppnvsvv
      661 lahgkhdskd lgdkgsesht rqeepregkq ddrnsrdfrd srdfresrdp vhsrdyrdsr
      721 dagqargrgs dgesldwkgs qdnfehdssr vgrgdlrddq drkrdryyer addrreaske
      781 drdeyrkagr kigttedrkr kddyyddrra dqledrredr regfrddyrk tdrrgdryhd
      841 rrdqwkgqes ddewkerrsd rrsdrysrhd yryddrhsdr ysdrgrpssr tsqdayldey
      901 dqrvgdrrvr dnhnrpssrh sesdssspds rrgygtgkhr kyreadrapg vyydhqksyg
      961 taggydynay rrdyynyyhy gyaprsgygy gyydelyrtn paykqqvday yarlgyspaq
     1021 lmerlsvhsg rssangdsrr desfasnted dcdpsildne strldhvpqp eevkkfsrph
     1081 plarfcgang llklvptlsg gnlpppieih slkhlfqkdp qfhelkqfpg plvrsqthkn
     1141 dviqfcthki qsfaedpnlp drsshillwe llvlmlrqng fvsgsdiael llrghnilep
     1201 vvsasglarr ggeggemtsp egaddgssps pdegivvsdr aqpgatgssq tlakfrefll
     1261 fghkkdalew avkcglwgha lslaskmdar tyasimtrft salatndplq tlyqhlsgrq
     1321 paavtciade kwgdwrphla milsnpssrp evdarsittl gdvlasrgcl saahfcylma
     1381 qvefgsysck ssklvllgss hqtllfeefa sneaiqctei yeysqslana gymlphlqay
     1441 kflhatrlae ygfaqealhy cevlaeamgr qcgsaqlaaq tyelasrlky hdphfsqgpg
     1501 eleelgeppw laafaqlvtq llqqhaaaap pqigalygqv sscggnavae vsggdsyldq
     1561 pgftsstgfs mhdsgmsile qsgnygnsyl dpsacgdsaq avspsadqwt alahtapdsr
     1621 sqhlpptsvp sfaatgtsve sgwtavqpsa eysfvstspp vesmvnggqt dgtapssppv
     1681 gsqtttfdyy kastysaqhp phhqtsrerl ssvssstdah drrdsvdsaa lasgrsrlps
     1741 gpqsplttdq sasesertkp aatnkgktqv agkswlggif gkllpkgpnq milpddkdpd
     1801 ivwdehkkcw vdknaapgda diqsaapptd ssfggkptpp sgkpgenrfq makqrslrkh
     1861 yvdvlnpgss kasgdspavp gvppvpeqpg tqsppqflvp qavddhcdsg ydfvspapmp
     1921 dhsapapppp ppetvsyqqp sgkvvstpmm fdpadfssra vdsshkmagg nlarrraypt
//
LOCUS       XP_077501114            1977 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X34 [Amblyomma
            americanum].
ACCESSION   XP_077501114
VERSION     XP_077501114.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644988.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1977
                     /product="uncharacterized protein LOC144111913 isoform
                     X34"
                     /calculated_mol_wt=214513
     Region          <335..642
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          605..>839
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1076..>1150
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1124..1488
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1246..1247,1249..1250,1252..1256,1258,1260,1270,
                     1272,1274..1275,1277..1278,1282,1288,1295,1304,1309,1312,
                     1340..1341)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1413,1435..1436,1438..1439,1442..1443,1446,
                     1475..1476,1479,1483)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1977
                     /gene="LOC144111913"
                     /coded_by="XM_077644988.1:93..6026"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqtccc lamqegwpsa vtgaangsta gsavhcggpa qgdnywnwaa hqepgaggdt
      121 aasapmqswn swgaaqevwt graqppdpat vggtneaava pvvndgtslp pqlgytsppe
      181 ggqqqygsgg vvaegalqeg waaphwsgdt nqwpsnleqq qfvtaqaqwq wqpadassav
      241 gqwqgygsql dpaaagpyes tslagyapld gslqqqqqqp gqdqlaassg envagtqhle
      301 atlgdngmvs mffqdggadd gqaesreggp tthdqhaeep gppshqqqta atgiaqdais
      361 qvapaassde svnqevlepv mrpepqdvlp lqaegsppag eeavlvmpeg qcpapakkav
      421 irarqggafk ppalarqgss stetalmqqn aiqlhlppen lellppdevg rhhaslrhrq
      481 gvaspaavga gltlldapem qpcvtlapaa ppiphdrpas rastgpqvap eatgceeees
      541 vpdghcqtpv trmvapplmr qsplgreegt grstaapaps lrvatvlaps plladttlpe
      601 rtepsgstdg dqpqpvvtaa ragedkeglp vprpkatstp tghcsahhep pnvsvvlahg
      661 khdskdlgdk gseshtrqee pregkqddrn srdfrdsrdf resrdpvhsr dyrdsrdagq
      721 argrgsdges ldwkgsqdnf ehdssrvgrg dlrddqdrkr dryyeraddr reaskedrde
      781 yrkagrkigt tedrkrkddy yddrradqle drredrregf rddyrktdrr gdryhdrrdq
      841 wkgqesddew kerrsdrrsd rysrhdyryd drhsdrysdr grpssrtsqd ayldeydqrv
      901 gdrrvrdnhn rpssrhsesd ssspdsrrgy gtgkhrkyre adrapgvyyd hqksygtagg
      961 ydynayrrdy ynyyhygyap rsgygygyyd elyrtnpayk qqvdayyarl gyspaqlmer
     1021 lsvhsgrssa ngdsrrdesf asnteddcdp sildnestrl dhvpqpeevk kfsrphplar
     1081 fcgangllkl vptlsggnlp ppieihslkh lfqkdpqfhe lkqfpgplvr sqthkndviq
     1141 fcthkiqsfa edpnlpdrss hillwellvl mlrqngfvsg sdiaelllrg hnilepvvsa
     1201 sglarrggeg gemtspegad dgsspspdeg ivvsdraqpg atgssqtlak frefllfghk
     1261 kdalewavkc glwghalsla skmdartyas imtrftsala tndplqtlyq hlsgrqpaav
     1321 tciadekwgd wrphlamils npssrpevda rsittlgdvl asrgclsaah fcylmaqvef
     1381 gsyscksskl vllgsshqtl lfeefasnea iqcteiyeys qslanagyml phlqaykflh
     1441 atrlaeygfa qealhycevl aeamgrqcgs aqlaaqtyel asrlkyhdph fsqgpgelee
     1501 lgeppwlaaf aqlvtqllqq haaaappqig alygqvsscg gnavaevsgg dsyldqpgft
     1561 sstgfsmhds gmsileqsgn ygnsyldpsa cgdsaqavsp sadqwtalah tapdsrsqhl
     1621 pptsvpsfaa tgtsvesgwt avqpsaeysf vstsppvesm vnggqtdgta pssppvgsqt
     1681 ttfdyykast ysqaqhpphh qtsrerlssv ssstdahdrr dsvdsaalas grsrlpsgpq
     1741 splttdqsas esertkpaat nkgktqvagk swlggifgkl lpkgpnqmil pddkdpdivw
     1801 dehkkcwvdk naapgdadiq saapptdssf ggkptppsgk pgenrfqmak qrslrkhyvd
     1861 vlnpgsskas gdspavpgvp pvpeqpgtqs ppqflvpqav ddhcdsgydf vspapmpdhs
     1921 apappppppe tvsyqqpsgk vvstpmmfdp adfssravds shkmaggnla rrraypt
//
LOCUS       XP_077501115            1974 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X35 [Amblyomma
            americanum].
ACCESSION   XP_077501115
VERSION     XP_077501115.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644989.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1974
                     /product="uncharacterized protein LOC144111913 isoform
                     X35"
                     /calculated_mol_wt=214288
     Region          <317..639
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          602..>836
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1073..>1147
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1121..1485
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1243..1244,1246..1247,1249..1253,1255,1257,1267,
                     1269,1271..1272,1274..1275,1279,1285,1292,1301,1306,1309,
                     1337..1338)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1410,1432..1433,1435..1436,1439..1440,1443,
                     1472..1473,1476,1480)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1974
                     /gene="LOC144111913"
                     /coded_by="XM_077644989.1:87..6011"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 mpqwdgstsf qptqypaall dpqqqqrqqq snlqegwpsa vtgaangsta gsavhcggpa
       61 qgdnywnwaa hqepgaggdt aasapmqswn swgaaqevwt graqppdpat vggtneaava
      121 pvvndgtslp pqlgytsppe ggqqqygsgg vvaegalqeg waaphwsgdt nqwpsnleqq
      181 qfvtaqaqwq wqpadassav gqwqgygsql dpaaagpyes tslagyapld gslqqqqqqp
      241 gqdqlaassg envagdmhlv ayssgpaavd gqggsegtqh leatlgdngm vsmffqdgga
      301 ddgqaesreg gptthdqhae epgppshqqq taatgiaqda isqvapaass desvnqevle
      361 pvmrpepqdv lplqaegspp ageeavlvmp egqcpapakk avirarqgga fkppalarqg
      421 ssstetdylr viyqllwylw halmqqnaiq lhlppenlel lppdevgrhh aslrhrqgva
      481 spaavgaglt lldapemqpc vtlapaappi phdrpasras tgpqvapeat gceeeesvpd
      541 ghcqtpvtrm vapplmrqsp lgreegtgrs taapapslrv atvlapspll adttlperte
      601 psgstdgdqp qpvvtaarag edkeglpvpr pkatstptgh csahheppnv svvlahgkhd
      661 skdlgdkgse shtrqeepre gkqddrnsrd frdsrdfres rdpvhsrdyr dsrdagqarg
      721 rgsdgesldw kgsqdnfehd ssrvgrgdlr ddqdrkrdry yeraddrrea skedrdeyrk
      781 agrkigtted rkrkddyydd rradqledrr edrregfrdd yrktdrrgdr yhdrrdqwkg
      841 qesddewker rsdrrsdrys rhdyryddrh sdrysdrgrp ssrtsqdayl deydqrvgdr
      901 rvrdnhnrps srhsesdsss pdsrrgygtg khrkyreadr apgvyydhqk sygtaggydy
      961 nayrrdyyny yhygyaprsg ygygyydely rtnpaykqqv dayyarlgys paqlmerlsv
     1021 hsgrssangd srrdesfasn teddcdpsil dnestrldhv pqpeevkkfs rphplarfcg
     1081 angllklvpt lsggnlpppi eihslkhlfq kdpqfhelkq fpgplvrsqt hkndviqfct
     1141 hkiqsfaedp nlpdrsshil lwellvlmlr qngfvsgsdi aelllrghni lepvvsasgl
     1201 arrggeggem tspegaddgs spspdegivv sdraqpgatg ssqtlakfre fllfghkkda
     1261 lewavkcglw ghalslaskm dartyasimt rftsalatnd plqtlyqhls grqpaavtci
     1321 adekwgdwrp hlamilsnps srpevdarsi ttlgdvlasr gclsaahfcy lmaqvefgsy
     1381 sckssklvll gsshqtllfe efasneaiqc teiyeysqsl anagymlphl qaykflhatr
     1441 laeygfaqea lhycevlaea mgrqcgsaql aaqtyelasr lkyhdphfsq gpgeleelge
     1501 ppwlaafaql vtqllqqhaa aappqigaly gqvsscggna vaevsggdsy ldqpgftsst
     1561 gfsmhdsgms ileqsgnygn syldpsacgd saqavspsad qwtalahtap dsrsqhlppt
     1621 svpsfaatgt svesgwtavq psaeysfvst sppvesmvng gqtdgtapss ppvgsqtttf
     1681 dyykastysq aqhpphhqts rerlssvsss tdahdrrdsv dsaalasgrs rlpsgpqspl
     1741 ttdqsasese rtkpaatnkg ktqvagkswl ggifgkllpk gpnqmilpdd kdpdivwdeh
     1801 kkcwvdknaa pgdadiqsaa pptdssfggk ptppsgkpge nrfqmakqrs lrkhyvdvln
     1861 pgsskasgds pavpgvppvp eqpgtqsppq flvpqavddh cdsgydfvsp apmpdhsapa
     1921 ppppppetvs yqqpsgkvvs tpmmfdpadf ssravdsshk maggnlarrr aypt
//
LOCUS       XP_077501117            1969 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X36 [Amblyomma
            americanum].
ACCESSION   XP_077501117
VERSION     XP_077501117.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644991.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1969
                     /product="uncharacterized protein LOC144111913 isoform
                     X36"
                     /calculated_mol_wt=213659
     Region          <327..634
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          597..>831
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1068..>1142
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1116..1480
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1238..1239,1241..1242,1244..1248,1250,1252,1262,
                     1264,1266..1267,1269..1270,1274,1280,1287,1296,1301,1304,
                     1332..1333)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1405,1427..1428,1430..1431,1434..1435,1438,
                     1467..1468,1471,1475)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1969
                     /gene="LOC144111913"
                     /coded_by="XM_077644991.1:93..6002"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqegwp savtgaangs tagsavhcgg paqgdnywnw aahqepgagg dtaasapmqs
      121 wnswgaaqev wtgraqppdp atvggtneaa vapvvndgts lppqlgytsp peggqqqygs
      181 ggvvaegalq egwaaphwsg dtnqwpsnle qqqfvtaqaq wqwqpadass avgqwqgygs
      241 qldpaaagpy estslagyap ldgslqqqqq qpgqdqlaas sgenvagtqh leatlgdngm
      301 vsmffqdgga ddgqaesreg gptthdqhae epgppshqqq taatgiaqda isqvapaass
      361 desvnqevle pvmrpepqdv lplqaegspp ageeavlvmp egqcpapakk avirarqgga
      421 fkppalarqg ssstetalmq qnaiqlhlpp enlellppde vgrhhaslrh rqgvaspaav
      481 gagltlldap emqpcvtlap aappiphdrp asrastgpqv apeatgceee esvpdghcqt
      541 pvtrmvappl mrqsplgree gtgrstaapa pslrvatvla psplladttl pertepsgst
      601 dgdqpqpvvt aaragedkeg lpvprpkats tptghcsahh eppnvsvvla hgkhdskdlg
      661 dkgseshtrq eepregkqdd rnsrdfrdsr dfresrdpvh srdyrdsrda gqargrgsdg
      721 esldwkgsqd nfehdssrvg rgdlrddqdr krdryyerad drreaskedr deyrkagrki
      781 gttedrkrkd dyyddrradq ledrredrre gfrddyrktd rrgdryhdrr dqwkgqesdd
      841 ewkerrsdrr sdrysrhdyr yddrhsdrys drgrpssrts qdayldeydq rvgdrrvrdn
      901 hnrpssrhse sdssspdsrr gygtgkhrky readrapgvy ydhqksygta ggydynayrr
      961 dyynyyhygy aprsgygygy ydelyrtnpa ykqqvdayya rlgyspaqlm erlsvhsgrs
     1021 sangdsrrde sfasnteddc dpsildnest rldhvpqpee vkkfsrphpl arfcgangll
     1081 klvptlsggn lpppieihsl khlfqkdpqf helkqfpgpl vrsqthkndv iqfcthkiqs
     1141 faedpnlpdr sshillwell vlmlrqngfv sgsdiaelll rghnilepvv sasglarrgg
     1201 eggemtspeg addgsspspd egivvsdraq pgatgssqtl akfrefllfg hkkdalewav
     1261 kcglwghals laskmdarty asimtrftsa latndplqtl yqhlsgrqpa avtciadekw
     1321 gdwrphlami lsnpssrpev darsittlgd vlasrgclsa ahfcylmaqv efgsysckss
     1381 klvllgsshq tllfeefasn eaiqcteiye ysqslanagy mlphlqaykf lhatrlaeyg
     1441 faqealhyce vlaeamgrqc gsaqlaaqty elasrlkyhd phfsqgpgel eelgeppwla
     1501 afaqlvtqll qqhaaaappq igalygqvss cggnavaevs ggdsyldqpg ftsstgfsmh
     1561 dsgmsileqs gnygnsyldp sacgdsaqav spsadqwtal ahtapdsrsq hlpptsvpsf
     1621 aatgtsvesg wtavqpsaey sfvstsppve smvnggqtdg tapssppvgs qtttfdyyka
     1681 stysqaqhpp hhqtsrerls svssstdahd rrdsvdsaal asgrsrlpsg pqsplttdqs
     1741 asesertkpa atnkgktqva gkswlggifg kllpkgpnqm ilpddkdpdi vwdehkkcwv
     1801 dknaapgdad iqsaapptds sfggkptpps gkpgenrfqm akqrslrkhy vdvlnpgssk
     1861 asgdspavpg vppvpeqpgt qsppqflvpq avddhcdsgy dfvspapmpd hsapappppp
     1921 petvsyqqps gkvvstpmmf dpadfssrav dsshkmaggn larrraypt
//
LOCUS       XP_077501118            1968 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X37 [Amblyomma
            americanum].
ACCESSION   XP_077501118
VERSION     XP_077501118.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644992.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1968
                     /product="uncharacterized protein LOC144111913 isoform
                     X37"
                     /calculated_mol_wt=213531
     Region          <327..634
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          597..>831
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1068..>1142
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1116..1480
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1238..1239,1241..1242,1244..1248,1250,1252,1262,
                     1264,1266..1267,1269..1270,1274,1280,1287,1296,1301,1304,
                     1332..1333)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1405,1427..1428,1430..1431,1434..1435,1438,
                     1467..1468,1471,1475)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1968
                     /gene="LOC144111913"
                     /coded_by="XM_077644992.1:93..5999"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqegwp savtgaangs tagsavhcgg paqgdnywnw aahqepgagg dtaasapmqs
      121 wnswgaaqev wtgraqppdp atvggtneaa vapvvndgts lppqlgytsp peggqqqygs
      181 ggvvaegalq egwaaphwsg dtnqwpsnle qqqfvtaqaq wqwqpadass avgqwqgygs
      241 qldpaaagpy estslagyap ldgslqqqqq qpgqdqlaas sgenvagtqh leatlgdngm
      301 vsmffqdgga ddgqaesreg gptthdqhae epgppshqqq taatgiaqda isqvapaass
      361 desvnqevle pvmrpepqdv lplqaegspp ageeavlvmp egqcpapakk avirarqgga
      421 fkppalarqg ssstetalmq qnaiqlhlpp enlellppde vgrhhaslrh rqgvaspaav
      481 gagltlldap emqpcvtlap aappiphdrp asrastgpqv apeatgceee esvpdghcqt
      541 pvtrmvappl mrqsplgree gtgrstaapa pslrvatvla psplladttl pertepsgst
      601 dgdqpqpvvt aaragedkeg lpvprpkats tptghcsahh eppnvsvvla hgkhdskdlg
      661 dkgseshtrq eepregkqdd rnsrdfrdsr dfresrdpvh srdyrdsrda gqargrgsdg
      721 esldwkgsqd nfehdssrvg rgdlrddqdr krdryyerad drreaskedr deyrkagrki
      781 gttedrkrkd dyyddrradq ledrredrre gfrddyrktd rrgdryhdrr dqwkgqesdd
      841 ewkerrsdrr sdrysrhdyr yddrhsdrys drgrpssrts qdayldeydq rvgdrrvrdn
      901 hnrpssrhse sdssspdsrr gygtgkhrky readrapgvy ydhqksygta ggydynayrr
      961 dyynyyhygy aprsgygygy ydelyrtnpa ykqqvdayya rlgyspaqlm erlsvhsgrs
     1021 sangdsrrde sfasnteddc dpsildnest rldhvpqpee vkkfsrphpl arfcgangll
     1081 klvptlsggn lpppieihsl khlfqkdpqf helkqfpgpl vrsqthkndv iqfcthkiqs
     1141 faedpnlpdr sshillwell vlmlrqngfv sgsdiaelll rghnilepvv sasglarrgg
     1201 eggemtspeg addgsspspd egivvsdraq pgatgssqtl akfrefllfg hkkdalewav
     1261 kcglwghals laskmdarty asimtrftsa latndplqtl yqhlsgrqpa avtciadekw
     1321 gdwrphlami lsnpssrpev darsittlgd vlasrgclsa ahfcylmaqv efgsysckss
     1381 klvllgsshq tllfeefasn eaiqcteiye ysqslanagy mlphlqaykf lhatrlaeyg
     1441 faqealhyce vlaeamgrqc gsaqlaaqty elasrlkyhd phfsqgpgel eelgeppwla
     1501 afaqlvtqll qqhaaaappq igalygqvss cggnavaevs ggdsyldqpg ftsstgfsmh
     1561 dsgmsileqs gnygnsyldp sacgdsaqav spsadqwtal ahtapdsrsq hlpptsvpsf
     1621 aatgtsvesg wtavqpsaey sfvstsppve smvnggqtdg tapssppvgs qtttfdyyka
     1681 stysaqhpph hqtsrerlss vssstdahdr rdsvdsaala sgrsrlpsgp qsplttdqsa
     1741 sesertkpaa tnkgktqvag kswlggifgk llpkgpnqmi lpddkdpdiv wdehkkcwvd
     1801 knaapgdadi qsaapptdss fggkptppsg kpgenrfqma kqrslrkhyv dvlnpgsska
     1861 sgdspavpgv ppvpeqpgtq sppqflvpqa vddhcdsgyd fvspapmpdh sapapppppp
     1921 etvsyqqpsg kvvstpmmfd padfssravd sshkmaggnl arrraypt
//
LOCUS       XP_077501119            1968 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X38 [Amblyomma
            americanum].
ACCESSION   XP_077501119
VERSION     XP_077501119.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644993.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1968
                     /product="uncharacterized protein LOC144111913 isoform
                     X38"
                     /calculated_mol_wt=214043
     Region          <311..633
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          596..>830
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1067..>1141
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1115..1479
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1237..1238,1240..1241,1243..1247,1249,1251,1261,
                     1263,1265..1266,1268..1269,1273,1279,1286,1295,1300,1303,
                     1331..1332)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1404,1426..1427,1429..1430,1433..1434,1437,
                     1466..1467,1470,1474)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1968
                     /gene="LOC144111913"
                     /coded_by="XM_077644993.1:93..5999"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqepga ggdtaasapm qswnswgaaq evwtgraqpp dpatvggtne aavapvvndg
      121 tslppqlgyt sppeggqqqy gsggvvaega lqegwaaphw sgdtnqwpsn leqqqfvtaq
      181 aqwqwqpada ssavgqwqgy gsqldpaaag pyestslagy apldgslqqq qqqpgqdqla
      241 assgenvagd mhlvayssgp aavdgqggse gtqhleatlg dngmvsmffq dggaddgqae
      301 sreggptthd qhaeepgpps hqqqtaatgi aqdaisqvap aassdesvnq evlepvmrpe
      361 pqdvlplqae gsppageeav lvmpegqcpa pakkavirar qggafkppal arqgssstet
      421 dylrviyqll wylwhalmqq naiqlhlppe nlellppdev grhhaslrhr qgvaspaavg
      481 agltlldape mqpcvtlapa appiphdrpa srastgpqva peatgceeee svpdghcqtp
      541 vtrmvapplm rqsplgreeg tgrstaapap slrvatvlap splladttlp ertepsgstd
      601 gdqpqpvvta aragedkegl pvprpkatst ptghcsahhe ppnvsvvlah gkhdskdlgd
      661 kgseshtrqe epregkqddr nsrdfrdsrd fresrdpvhs rdyrdsrdag qargrgsdge
      721 sldwkgsqdn fehdssrvgr gdlrddqdrk rdryyeradd rreaskedrd eyrkagrkig
      781 ttedrkrkdd yyddrradql edrredrreg frddyrktdr rgdryhdrrd qwkgqesdde
      841 wkerrsdrrs drysrhdyry ddrhsdrysd rgrpssrtsq dayldeydqr vgdrrvrdnh
      901 nrpssrhses dssspdsrrg ygtgkhrkyr eadrapgvyy dhqksygtag gydynayrrd
      961 yynyyhygya prsgygygyy delyrtnpay kqqvdayyar lgyspaqlme rlsvhsgrss
     1021 angdsrrdes fasnteddcd psildnestr ldhvpqpeev kkfsrphpla rfcgangllk
     1081 lvptlsggnl pppieihslk hlfqkdpqfh elkqfpgplv rsqthkndvi qfcthkiqsf
     1141 aedpnlpdrs shillwellv lmlrqngfvs gsdiaelllr ghnilepvvs asglarrgge
     1201 ggemtspega ddgsspspde givvsdraqp gatgssqtla kfrefllfgh kkdalewavk
     1261 cglwghalsl askmdartya simtrftsal atndplqtly qhlsgrqpaa vtciadekwg
     1321 dwrphlamil snpssrpevd arsittlgdv lasrgclsaa hfcylmaqve fgsysckssk
     1381 lvllgsshqt llfeefasne aiqcteiyey sqslanagym lphlqaykfl hatrlaeygf
     1441 aqealhycev laeamgrqcg saqlaaqtye lasrlkyhdp hfsqgpgele elgeppwlaa
     1501 faqlvtqllq qhaaaappqi galygqvssc ggnavaevsg gdsyldqpgf tsstgfsmhd
     1561 sgmsileqsg nygnsyldps acgdsaqavs psadqwtala htapdsrsqh lpptsvpsfa
     1621 atgtsvesgw tavqpsaeys fvstsppves mvnggqtdgt apssppvgsq tttfdyykas
     1681 tysqaqhpph hqtsrerlss vssstdahdr rdsvdsaala sgrsrlpsgp qsplttdqsa
     1741 sesertkpaa tnkgktqvag kswlggifgk llpkgpnqmi lpddkdpdiv wdehkkcwvd
     1801 knaapgdadi qsaapptdss fggkptppsg kpgenrfqma kqrslrkhyv dvlnpgsska
     1861 sgdspavpgv ppvpeqpgtq sppqflvpqa vddhcdsgyd fvspapmpdh sapapppppp
     1921 etvsyqqpsg kvvstpmmfd padfssravd sshkmaggnl arrraypt
//
LOCUS       XP_077501120            1968 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X39 [Amblyomma
            americanum].
ACCESSION   XP_077501120
VERSION     XP_077501120.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644994.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1968
                     /product="uncharacterized protein LOC144111913 isoform
                     X39"
                     /calculated_mol_wt=213310
     Region          <357..664
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          627..>861
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1098..>1172
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1146..1510
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1268..1269,1271..1272,1274..1278,1280,1282,1292,
                     1294,1296..1297,1299..1300,1304,1310,1317,1326,1331,1334,
                     1362..1363)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1435,1457..1458,1460..1461,1464..1465,1468,
                     1497..1498,1501,1505)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1968
                     /gene="LOC144111913"
                     /coded_by="XM_077644994.1:93..5999"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqtccc lamqegwpsa vtgaangsta gsavhcggpa qgdnywnwaa hqepgaggdt
      121 aasapmqswn swgaaqevwt graqppdpat vggtneaava pvvndgtslp pqlgytsppe
      181 ggqqqygsgg vvaegalqeg waaphwsgdt nqwpsnleqq qfvtaqaqwq wqpadassav
      241 gqwqgygsql dpaaagpyes tslagyapld gslqqqqqqp gqdqlaassg envagdmhlv
      301 ayssgpaavd gqggsegtqh leatlgdngm vsmffqdgga ddgqaesreg gptthdqhae
      361 epgppshqqq taatgiaqda isqvapaass desvnqevle pvmrpepqdv lplqaegspp
      421 ageeavlvmp egqcpapakk avirarqgga fkppalarqg ssstetalmq qnaiqlhlpp
      481 enlellppde vgrhhaslrh rqgvaspaav gagltlldap emqpcvtlap aappiphdrp
      541 asrastgpqv apeatgceee esvpdghcqt pvtrmvappl mrqsplgree gtgrstaapa
      601 pslrvatvla psplladttl pertepsgst dgdqpqpvvt aaragedkeg lpvprpkats
      661 tptghcsahh eppnvsvvla hgkhdskdlg dkgseshtrq eepregkqdd rnsrdfrdsr
      721 dfresrdpvh srdyrdsrda gqargrgsdg esldwkgsqd nfehdssrvg rgdlrddqdr
      781 krdryyerad drreaskedr deyrkagrki gttedrkrkd dyyddrradq ledrredrre
      841 gfrddyrktd rrgdryhdrr dqwkgqesdd ewkerrsdrr sdrysrhdyr yddrhsdrys
      901 drgrpssrts qdayldeydq rvgdrrvrdn hnrpssrhse sdssspdsrr gygtgkhrky
      961 readrapgvy ydhqksygta ggydynayrr dyynyyhygy aprsgygygy ydelyrtnpa
     1021 ykqqvdayya rlgyspaqlm erlsvhsgrs sangdsrrde sfasnteddc dpsildnest
     1081 rldhvpqpee vkkfsrphpl arfcgangll klvptlsggn lpppieihsl khlfqkdpqf
     1141 helkqfpgpl vrsqthkndv iqfcthkiqs faedpnlpdr sshillwell vlmlrqngfv
     1201 sgsdiaelll rghnilepvv sasglarrgg eggemtspeg addgsspspd egivvsdraq
     1261 pgatgssqtl akfrefllfg hkkdalewav kcglwghals laskmdarty asimtrftsa
     1321 latndplqtl yqhlsgrqpa avtciadekw gdwrphlami lsnpssrpev darsittlgd
     1381 vlasrgclsa ahfcylmaqv efgsysckss klvllgsshq tllfeefasn eaiqcteiye
     1441 ysqslanagy mlphlqaykf lhatrlaeyg faqealhyce vlaeamgrqc gsaqlaaqty
     1501 elasrlkyhd phfsqgpgel eelgeppwla afaqlvtqll qqhaaaappq igalygqvss
     1561 cggnavaevs ggdsyldqpg ftsstgfsmh dsgmsileqs gnygnsyldp sacgdsaqav
     1621 spsadqwtal ahtapdsrsq hlpptsvpsf aatgtsvesg wtavqpsaey sfvstsppve
     1681 smvnggqtdg tapssppvgs qtttfdyyka stysqaqhpp hhqtsrerls svssstdahd
     1741 rrdsvdsaal asgrsrlpsg pqsplttdqs asesertkpa atnkgktqiv wdehkkcwvd
     1801 knaapgdadi qsaapptdss fggkptppsg kpgenrfqma kqrslrkhyv dvlnpgsska
     1861 sgdspavpgv ppvpeqpgtq sppqflvpqa vddhcdsgyd fvspapmpdh sapapppppp
     1921 etvsyqqpsg kvvstpmmfd padfssravd sshkmaggnl arrraypt
//
LOCUS       XP_077501121            1967 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X40 [Amblyomma
            americanum].
ACCESSION   XP_077501121
VERSION     XP_077501121.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644995.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1967
                     /product="uncharacterized protein LOC144111913 isoform
                     X40"
                     /calculated_mol_wt=213182
     Region          <357..664
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          627..>861
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1098..>1172
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1146..1510
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1268..1269,1271..1272,1274..1278,1280,1282,1292,
                     1294,1296..1297,1299..1300,1304,1310,1317,1326,1331,1334,
                     1362..1363)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1435,1457..1458,1460..1461,1464..1465,1468,
                     1497..1498,1501,1505)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1967
                     /gene="LOC144111913"
                     /coded_by="XM_077644995.1:93..5996"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqtccc lamqegwpsa vtgaangsta gsavhcggpa qgdnywnwaa hqepgaggdt
      121 aasapmqswn swgaaqevwt graqppdpat vggtneaava pvvndgtslp pqlgytsppe
      181 ggqqqygsgg vvaegalqeg waaphwsgdt nqwpsnleqq qfvtaqaqwq wqpadassav
      241 gqwqgygsql dpaaagpyes tslagyapld gslqqqqqqp gqdqlaassg envagdmhlv
      301 ayssgpaavd gqggsegtqh leatlgdngm vsmffqdgga ddgqaesreg gptthdqhae
      361 epgppshqqq taatgiaqda isqvapaass desvnqevle pvmrpepqdv lplqaegspp
      421 ageeavlvmp egqcpapakk avirarqgga fkppalarqg ssstetalmq qnaiqlhlpp
      481 enlellppde vgrhhaslrh rqgvaspaav gagltlldap emqpcvtlap aappiphdrp
      541 asrastgpqv apeatgceee esvpdghcqt pvtrmvappl mrqsplgree gtgrstaapa
      601 pslrvatvla psplladttl pertepsgst dgdqpqpvvt aaragedkeg lpvprpkats
      661 tptghcsahh eppnvsvvla hgkhdskdlg dkgseshtrq eepregkqdd rnsrdfrdsr
      721 dfresrdpvh srdyrdsrda gqargrgsdg esldwkgsqd nfehdssrvg rgdlrddqdr
      781 krdryyerad drreaskedr deyrkagrki gttedrkrkd dyyddrradq ledrredrre
      841 gfrddyrktd rrgdryhdrr dqwkgqesdd ewkerrsdrr sdrysrhdyr yddrhsdrys
      901 drgrpssrts qdayldeydq rvgdrrvrdn hnrpssrhse sdssspdsrr gygtgkhrky
      961 readrapgvy ydhqksygta ggydynayrr dyynyyhygy aprsgygygy ydelyrtnpa
     1021 ykqqvdayya rlgyspaqlm erlsvhsgrs sangdsrrde sfasnteddc dpsildnest
     1081 rldhvpqpee vkkfsrphpl arfcgangll klvptlsggn lpppieihsl khlfqkdpqf
     1141 helkqfpgpl vrsqthkndv iqfcthkiqs faedpnlpdr sshillwell vlmlrqngfv
     1201 sgsdiaelll rghnilepvv sasglarrgg eggemtspeg addgsspspd egivvsdraq
     1261 pgatgssqtl akfrefllfg hkkdalewav kcglwghals laskmdarty asimtrftsa
     1321 latndplqtl yqhlsgrqpa avtciadekw gdwrphlami lsnpssrpev darsittlgd
     1381 vlasrgclsa ahfcylmaqv efgsysckss klvllgsshq tllfeefasn eaiqcteiye
     1441 ysqslanagy mlphlqaykf lhatrlaeyg faqealhyce vlaeamgrqc gsaqlaaqty
     1501 elasrlkyhd phfsqgpgel eelgeppwla afaqlvtqll qqhaaaappq igalygqvss
     1561 cggnavaevs ggdsyldqpg ftsstgfsmh dsgmsileqs gnygnsyldp sacgdsaqav
     1621 spsadqwtal ahtapdsrsq hlpptsvpsf aatgtsvesg wtavqpsaey sfvstsppve
     1681 smvnggqtdg tapssppvgs qtttfdyyka stysaqhpph hqtsrerlss vssstdahdr
     1741 rdsvdsaala sgrsrlpsgp qsplttdqsa sesertkpaa tnkgktqivw dehkkcwvdk
     1801 naapgdadiq saapptdssf ggkptppsgk pgenrfqmak qrslrkhyvd vlnpgsskas
     1861 gdspavpgvp pvpeqpgtqs ppqflvpqav ddhcdsgydf vspapmpdhs apappppppe
     1921 tvsyqqpsgk vvstpmmfdp adfssravds shkmaggnla rrraypt
//
LOCUS       XP_077501122            1965 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X41 [Amblyomma
            americanum].
ACCESSION   XP_077501122
VERSION     XP_077501122.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644996.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1965
                     /product="uncharacterized protein LOC144111913 isoform
                     X41"
                     /calculated_mol_wt=212915
     Region          <324..631
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          594..>828
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1065..>1139
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1113..1477
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1235..1236,1238..1239,1241..1245,1247,1249,1259,
                     1261,1263..1264,1266..1267,1271,1277,1284,1293,1298,1301,
                     1329..1330)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1402,1424..1425,1427..1428,1431..1432,1435,
                     1464..1465,1468,1472)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1965
                     /gene="LOC144111913"
                     /coded_by="XM_077644996.1:21..5918"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 mdcwpvgatg kaaeaaqpak tlpqpvgevm pqwdgstsfq ptqypaalld pqqqqrqqqs
       61 nlqegwpsav tgaangstag savhcggpaq gdnywnwaah qepgaggdta asapmqswns
      121 wgaaqevwtg raqppdpatv ggtneaavap vvndgtslpp qlgytsppeg gqqqygsggv
      181 vaegalqegw aaphwsgdtn qwpsnleqqq fvtaqaqwqw qpadassavg qwqgygsqld
      241 paaagpyest slagyapldg slqqqqqqpg qdqlaassge nvagtqhlea tlgdngmvsm
      301 ffqdggaddg qaesreggpt thdqhaeepg ppshqqqtaa tgiaqdaisq vapaassdes
      361 vnqevlepvm rpepqdvlpl qaegsppage eavlvmpegq cpapakkavi rarqggafkp
      421 palarqgsss tetalmqqna iqlhlppenl ellppdevgr hhaslrhrqg vaspaavgag
      481 ltlldapemq pcvtlapaap piphdrpasr astgpqvape atgceeeesv pdghcqtpvt
      541 rmvapplmrq splgreegtg rstaapapsl rvatvlapsp lladttlper tepsgstdgd
      601 qpqpvvtaar agedkeglpv prpkatstpt ghcsahhepp nvsvvlahgk hdskdlgdkg
      661 seshtrqeep regkqddrns rdfrdsrdfr esrdpvhsrd yrdsrdagqa rgrgsdgesl
      721 dwkgsqdnfe hdssrvgrgd lrddqdrkrd ryyeraddrr easkedrdey rkagrkigtt
      781 edrkrkddyy ddrradqled rredrregfr ddyrktdrrg dryhdrrdqw kgqesddewk
      841 errsdrrsdr ysrhdyrydd rhsdrysdrg rpssrtsqda yldeydqrvg drrvrdnhnr
      901 pssrhsesds sspdsrrgyg tgkhrkyrea drapgvyydh qksygtaggy dynayrrdyy
      961 nyyhygyapr sgygygyyde lyrtnpaykq qvdayyarlg yspaqlmerl svhsgrssan
     1021 gdsrrdesfa snteddcdps ildnestrld hvpqpeevkk fsrphplarf cgangllklv
     1081 ptlsggnlpp pieihslkhl fqkdpqfhel kqfpgplvrs qthkndviqf cthkiqsfae
     1141 dpnlpdrssh illwellvlm lrqngfvsgs diaelllrgh nilepvvsas glarrggegg
     1201 emtspegadd gsspspdegi vvsdraqpga tgssqtlakf refllfghkk dalewavkcg
     1261 lwghalslas kmdartyasi mtrftsalat ndplqtlyqh lsgrqpaavt ciadekwgdw
     1321 rphlamilsn pssrpevdar sittlgdvla srgclsaahf cylmaqvefg sysckssklv
     1381 llgsshqtll feefasneai qcteiyeysq slanagymlp hlqaykflha trlaeygfaq
     1441 ealhycevla eamgrqcgsa qlaaqtyela srlkyhdphf sqgpgeleel geppwlaafa
     1501 qlvtqllqqh aaaappqiga lygqvsscgg navaevsggd syldqpgfts stgfsmhdsg
     1561 msileqsgny gnsyldpsac gdsaqavsps adqwtalaht apdsrsqhlp ptsvpsfaat
     1621 gtsvesgwta vqpsaeysfv stsppvesmv nggqtdgtap ssppvgsqtt tfdyykasty
     1681 saqhpphhqt srerlssvss stdahdrrds vdsaalasgr srlpsgpqsp lttdqsases
     1741 ertkpaatnk gktqvagksw lggifgkllp kgpnqmilpd dkdpdivwde hkkcwvdkna
     1801 apgdadiqsa apptdssfgg kptppsgkpg enrfqmakqr slrkhyvdvl npgsskasgd
     1861 spavpgvppv peqpgtqspp qflvpqavdd hcdsgydfvs papmpdhsap appppppetv
     1921 syqqpsgkvv stpmmfdpad fssravdssh kmaggnlarr raypt
//
LOCUS       XP_077501123            1964 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X42 [Amblyomma
            americanum].
ACCESSION   XP_077501123
VERSION     XP_077501123.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644997.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1964
                     /product="uncharacterized protein LOC144111913 isoform
                     X42"
                     /calculated_mol_wt=212787
     Region          <324..631
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          594..>828
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1065..>1139
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1113..1477
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1235..1236,1238..1239,1241..1245,1247,1249,1259,
                     1261,1263..1264,1266..1267,1271,1277,1284,1293,1298,1301,
                     1329..1330)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1402,1424..1425,1427..1428,1431..1432,1435,
                     1464..1465,1468,1472)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1964
                     /gene="LOC144111913"
                     /coded_by="XM_077644997.1:22..5916"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 mdcwpvgatg kaaeaaqpak tlpqpvgevm pqwdgstsfq ptqypaalld pqqqqrqqqs
       61 nlqegwpsav tgaangstag savhcggpaq gdnywnwaah qepgaggdta asapmqswns
      121 wgaaqevwtg raqppdpatv ggtneaavap vvndgtslpp qlgytsppeg gqqqygsggv
      181 vaegalqegw aaphwsgdtn qwpsnleqqq fvtaqaqwqw qpadassavg qwqgygsqld
      241 paaagpyest slagyapldg slqqqqqqpg qdqlaassge nvagtqhlea tlgdngmvsm
      301 ffqdggaddg qaesreggpt thdqhaeepg ppshqqqtaa tgiaqdaisq vapaassdes
      361 vnqevlepvm rpepqdvlpl qaegsppage eavlvmpegq cpapakkavi rarqggafkp
      421 palarqgsss tetalmqqna iqlhlppenl ellppdevgr hhaslrhrqg vaspaavgag
      481 ltlldapemq pcvtlapaap piphdrpasr astgpqvape atgceeeesv pdghcqtpvt
      541 rmvapplmrq splgreegtg rstaapapsl rvatvlapsp lladttlper tepsgstdgd
      601 qpqpvvtaar agedkeglpv prpkatstpt ghcsahhepp nvsvvlahgk hdskdlgdkg
      661 seshtrqeep regkqddrns rdfrdsrdfr esrdpvhsrd yrdsrdagqa rgrgsdgesl
      721 dwkgsqdnfe hdssrvgrgd lrddqdrkrd ryyeraddrr easkedrdey rkagrkigtt
      781 edrkrkddyy ddrradqled rredrregfr ddyrktdrrg dryhdrrdqw kgqesddewk
      841 errsdrrsdr ysrhdyrydd rhsdrysdrg rpssrtsqda yldeydqrvg drrvrdnhnr
      901 pssrhsesds sspdsrrgyg tgkhrkyrea drapgvyydh qksygtaggy dynayrrdyy
      961 nyyhygyapr sgygygyyde lyrtnpaykq qvdayyarlg yspaqlmerl svhsgrssan
     1021 gdsrrdesfa snteddcdps ildnestrld hvpqpeevkk fsrphplarf cgangllklv
     1081 ptlsggnlpp pieihslkhl fqkdpqfhel kqfpgplvrs qthkndviqf cthkiqsfae
     1141 dpnlpdrssh illwellvlm lrqngfvsgs diaelllrgh nilepvvsas glarrggegg
     1201 emtspegadd gsspspdegi vvsdraqpga tgssqtlakf refllfghkk dalewavkcg
     1261 lwghalslas kmdartyasi mtrftsalat ndplqtlyqh lsgrqpaavt ciadekwgdw
     1321 rphlamilsn pssrpevdar sittlgdvla srgclsaahf cylmaqvefg sysckssklv
     1381 llgsshqtll feefasneai qcteiyeysq slanagymlp hlqaykflha trlaeygfaq
     1441 ealhycevla eamgrqcgsa qlaaqtyela srlkyhdphf sqgpgeleel geppwlaafa
     1501 qlvtqllqqh aaaappqiga lygqvsscgg navaevsggd syldqpgfts stgfsmhdsg
     1561 msileqsgny gnsyldpsac gdsaqavsps adqwtalaht apdsrsqhlp ptsvpsfaat
     1621 gtsvesgwta vqpsaeysfv stsppvesmv nggqtdgtap ssppvgsqtt tfdyykasty
     1681 saqhpphhqt srerlssvss stdahdrrds vdsaalasgr srlpsgpqsp lttdqsases
     1741 ertkpaatnk gktqvagksw lggifgkllp kgpnqmilpd dkdpdivwde hkkcwvdkna
     1801 apgdadiqsa apptdssfgg kptppsgkpg enrfqmakqr slrkhyvdvl npgsskasgd
     1861 spavpgvppv peqpgtqspp qflvpqavdd hcdsgydfvs papmpdhsap appppppetv
     1921 syqpsgkvvs tpmmfdpadf ssravdsshk maggnlarrr aypt
//
LOCUS       XP_077501124            1962 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X43 [Amblyomma
            americanum].
ACCESSION   XP_077501124
VERSION     XP_077501124.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644998.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1962
                     /product="uncharacterized protein LOC144111913 isoform
                     X43"
                     /calculated_mol_wt=212868
     Region          <305..627
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          590..>824
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1061..>1135
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1109..1473
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1231..1232,1234..1235,1237..1241,1243,1245,1255,
                     1257,1259..1260,1262..1263,1267,1273,1280,1289,1294,1297,
                     1325..1326)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1398,1420..1421,1423..1424,1427..1428,1431,
                     1460..1461,1464,1468)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1962
                     /gene="LOC144111913"
                     /coded_by="XM_077644998.1:315..6203"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 maallvlqst agaqhrgttt gtglltrler aslsqpaspp pplrkapswi saealrwtgl
       61 epgaggdtaa sapmqswnsw gaaqevwtgr aqppdpatvg gtneaavapv vndgtslppq
      121 lgytsppegg qqqygsggvv aegalqegwa aphwsgdtnq wpsnleqqqf vtaqaqwqwq
      181 padassavgq wqgygsqldp aaagpyests lagyapldgs lqqqqqqpgq dqlaassgen
      241 vagdmhlvay ssgpaavdgq ggsegtqhle atlgdngmvs mffqdggadd gqaesreggp
      301 tthdqhaeep gppshqqqta atgiaqdais qvapaassde svnqevlepv mrpepqdvlp
      361 lqaegsppag eeavlvmpeg qcpapakkav irarqggafk ppalarqgss stetdylrvi
      421 yqllwylwha lmqqnaiqlh lppenlellp pdevgrhhas lrhrqgvasp aavgagltll
      481 dapemqpcvt lapaappiph drpasrastg pqvapeatgc eeeesvpdgh cqtpvtrmva
      541 pplmrqsplg reegtgrsta apapslrvat vlapspllad ttlperteps gstdgdqpqp
      601 vvtaaraged keglpvprpk atstptghcs ahheppnvsv vlahgkhdsk dlgdkgsesh
      661 trqeepregk qddrnsrdfr dsrdfresrd pvhsrdyrds rdagqargrg sdgesldwkg
      721 sqdnfehdss rvgrgdlrdd qdrkrdryye raddrreask edrdeyrkag rkigttedrk
      781 rkddyyddrr adqledrred rregfrddyr ktdrrgdryh drrdqwkgqe sddewkerrs
      841 drrsdrysrh dyryddrhsd rysdrgrpss rtsqdaylde ydqrvgdrrv rdnhnrpssr
      901 hsesdssspd srrgygtgkh rkyreadrap gvyydhqksy gtaggydyna yrrdyynyyh
      961 ygyaprsgyg ygyydelyrt npaykqqvda yyarlgyspa qlmerlsvhs grssangdsr
     1021 rdesfasnte ddcdpsildn estrldhvpq peevkkfsrp hplarfcgan gllklvptls
     1081 ggnlpppiei hslkhlfqkd pqfhelkqfp gplvrsqthk ndviqfcthk iqsfaedpnl
     1141 pdrsshillw ellvlmlrqn gfvsgsdiae lllrghnile pvvsasglar rggeggemts
     1201 pegaddgssp spdegivvsd raqpgatgss qtlakfrefl lfghkkdale wavkcglwgh
     1261 alslaskmda rtyasimtrf tsalatndpl qtlyqhlsgr qpaavtciad ekwgdwrphl
     1321 amilsnpssr pevdarsitt lgdvlasrgc lsaahfcylm aqvefgsysc kssklvllgs
     1381 shqtllfeef asneaiqcte iyeysqslan agymlphlqa ykflhatrla eygfaqealh
     1441 ycevlaeamg rqcgsaqlaa qtyelasrlk yhdphfsqgp geleelgepp wlaafaqlvt
     1501 qllqqhaaaa ppqigalygq vsscggnava evsggdsyld qpgftsstgf smhdsgmsil
     1561 eqsgnygnsy ldpsacgdsa qavspsadqw talahtapds rsqhlpptsv psfaatgtsv
     1621 esgwtavqps aeysfvstsp pvesmvnggq tdgtapsspp vgsqtttfdy ykastysqaq
     1681 hpphhqtsre rlssvssstd ahdrrdsvds aalasgrsrl psgpqspltt dqsasesert
     1741 kpaatnkgkt qvagkswlgg ifgkllpkgp nqmilpddkd pdivwdehkk cwvdknaapg
     1801 dadiqsaapp tdssfggkpt ppsgkpgenr fqmakqrslr khyvdvlnpg sskasgdspa
     1861 vpgvppvpeq pgtqsppqfl vpqavddhcd sgydfvspap mpdhsapapp ppppetvsyq
     1921 qpsgkvvstp mmfdpadfss ravdsshkma ggnlarrray pt
//
LOCUS       XP_077501125            1962 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X43 [Amblyomma
            americanum].
ACCESSION   XP_077501125
VERSION     XP_077501125.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644999.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1962
                     /product="uncharacterized protein LOC144111913 isoform
                     X43"
                     /calculated_mol_wt=212868
     Region          <305..627
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          590..>824
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1061..>1135
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1109..1473
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1231..1232,1234..1235,1237..1241,1243,1245,1255,
                     1257,1259..1260,1262..1263,1267,1273,1280,1289,1294,1297,
                     1325..1326)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1398,1420..1421,1423..1424,1427..1428,1431,
                     1460..1461,1464,1468)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1962
                     /gene="LOC144111913"
                     /coded_by="XM_077644999.1:162..6050"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 maallvlqst agaqhrgttt gtglltrler aslsqpaspp pplrkapswi saealrwtgl
       61 epgaggdtaa sapmqswnsw gaaqevwtgr aqppdpatvg gtneaavapv vndgtslppq
      121 lgytsppegg qqqygsggvv aegalqegwa aphwsgdtnq wpsnleqqqf vtaqaqwqwq
      181 padassavgq wqgygsqldp aaagpyests lagyapldgs lqqqqqqpgq dqlaassgen
      241 vagdmhlvay ssgpaavdgq ggsegtqhle atlgdngmvs mffqdggadd gqaesreggp
      301 tthdqhaeep gppshqqqta atgiaqdais qvapaassde svnqevlepv mrpepqdvlp
      361 lqaegsppag eeavlvmpeg qcpapakkav irarqggafk ppalarqgss stetdylrvi
      421 yqllwylwha lmqqnaiqlh lppenlellp pdevgrhhas lrhrqgvasp aavgagltll
      481 dapemqpcvt lapaappiph drpasrastg pqvapeatgc eeeesvpdgh cqtpvtrmva
      541 pplmrqsplg reegtgrsta apapslrvat vlapspllad ttlperteps gstdgdqpqp
      601 vvtaaraged keglpvprpk atstptghcs ahheppnvsv vlahgkhdsk dlgdkgsesh
      661 trqeepregk qddrnsrdfr dsrdfresrd pvhsrdyrds rdagqargrg sdgesldwkg
      721 sqdnfehdss rvgrgdlrdd qdrkrdryye raddrreask edrdeyrkag rkigttedrk
      781 rkddyyddrr adqledrred rregfrddyr ktdrrgdryh drrdqwkgqe sddewkerrs
      841 drrsdrysrh dyryddrhsd rysdrgrpss rtsqdaylde ydqrvgdrrv rdnhnrpssr
      901 hsesdssspd srrgygtgkh rkyreadrap gvyydhqksy gtaggydyna yrrdyynyyh
      961 ygyaprsgyg ygyydelyrt npaykqqvda yyarlgyspa qlmerlsvhs grssangdsr
     1021 rdesfasnte ddcdpsildn estrldhvpq peevkkfsrp hplarfcgan gllklvptls
     1081 ggnlpppiei hslkhlfqkd pqfhelkqfp gplvrsqthk ndviqfcthk iqsfaedpnl
     1141 pdrsshillw ellvlmlrqn gfvsgsdiae lllrghnile pvvsasglar rggeggemts
     1201 pegaddgssp spdegivvsd raqpgatgss qtlakfrefl lfghkkdale wavkcglwgh
     1261 alslaskmda rtyasimtrf tsalatndpl qtlyqhlsgr qpaavtciad ekwgdwrphl
     1321 amilsnpssr pevdarsitt lgdvlasrgc lsaahfcylm aqvefgsysc kssklvllgs
     1381 shqtllfeef asneaiqcte iyeysqslan agymlphlqa ykflhatrla eygfaqealh
     1441 ycevlaeamg rqcgsaqlaa qtyelasrlk yhdphfsqgp geleelgepp wlaafaqlvt
     1501 qllqqhaaaa ppqigalygq vsscggnava evsggdsyld qpgftsstgf smhdsgmsil
     1561 eqsgnygnsy ldpsacgdsa qavspsadqw talahtapds rsqhlpptsv psfaatgtsv
     1621 esgwtavqps aeysfvstsp pvesmvnggq tdgtapsspp vgsqtttfdy ykastysqaq
     1681 hpphhqtsre rlssvssstd ahdrrdsvds aalasgrsrl psgpqspltt dqsasesert
     1741 kpaatnkgkt qvagkswlgg ifgkllpkgp nqmilpddkd pdivwdehkk cwvdknaapg
     1801 dadiqsaapp tdssfggkpt ppsgkpgenr fqmakqrslr khyvdvlnpg sskasgdspa
     1861 vpgvppvpeq pgtqsppqfl vpqavddhcd sgydfvspap mpdhsapapp ppppetvsyq
     1921 qpsgkvvstp mmfdpadfss ravdsshkma ggnlarrray pt
//
LOCUS       XP_077501126            1959 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X44 [Amblyomma
            americanum].
ACCESSION   XP_077501126
VERSION     XP_077501126.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645000.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1959
                     /product="uncharacterized protein LOC144111913 isoform
                     X44"
                     /calculated_mol_wt=212225
     Region          <317..624
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          587..>821
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1058..>1132
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1106..1470
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1228..1229,1231..1232,1234..1238,1240,1242,1252,
                     1254,1256..1257,1259..1260,1264,1270,1277,1286,1291,1294,
                     1322..1323)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1395,1417..1418,1420..1421,1424..1425,1428,
                     1457..1458,1461,1465)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1959
                     /gene="LOC144111913"
                     /coded_by="XM_077645000.1:115..5994"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 mpqwdgstsf qptqypaall dpqqqqrqqq snlqegwpsa vtgaangsta gsavhcggpa
       61 qgdnywnwaa hqepgaggdt aasapmqswn swgaaqevwt graqppdpat vggtneaava
      121 pvvndgtslp pqlgytsppe ggqqqygsgg vvaegalqeg waaphwsgdt nqwpsnleqq
      181 qfvtaqaqwq wqpadassav gqwqgygsql dpaaagpyes tslagyapld gslqqqqqqp
      241 gqdqlaassg envagdmhlv ayssgpaavd gqggsegtqh leatlgdngm vsmffqdgga
      301 ddgqaesreg gptthdqhae epgppshqqq taatgiaqda isqvapaass desvnqevle
      361 pvmrpepqdv lplqaegspp ageeavlvmp egqcpapakk avirarqgga fkppalarqg
      421 ssstetalmq qnaiqlhlpp enlellppde vgrhhaslrh rqgvaspaav gagltlldap
      481 emqpcvtlap aappiphdrp asrastgpqv apeatgceee esvpdghcqt pvtrmvappl
      541 mrqsplgree gtgrstaapa pslrvatvla psplladttl pertepsgst dgdqpqpvvt
      601 aaragedkeg lpvprpkats tptghcsahh eppnvsvvla hgkhdskdlg dkgseshtrq
      661 eepregkqdd rnsrdfrdsr dfresrdpvh srdyrdsrda gqargrgsdg esldwkgsqd
      721 nfehdssrvg rgdlrddqdr krdryyerad drreaskedr deyrkagrki gttedrkrkd
      781 dyyddrradq ledrredrre gfrddyrktd rrgdryhdrr dqwkgqesdd ewkerrsdrr
      841 sdrysrhdyr yddrhsdrys drgrpssrts qdayldeydq rvgdrrvrdn hnrpssrhse
      901 sdssspdsrr gygtgkhrky readrapgvy ydhqksygta ggydynayrr dyynyyhygy
      961 aprsgygygy ydelyrtnpa ykqqvdayya rlgyspaqlm erlsvhsgrs sangdsrrde
     1021 sfasnteddc dpsildnest rldhvpqpee vkkfsrphpl arfcgangll klvptlsggn
     1081 lpppieihsl khlfqkdpqf helkqfpgpl vrsqthkndv iqfcthkiqs faedpnlpdr
     1141 sshillwell vlmlrqngfv sgsdiaelll rghnilepvv sasglarrgg eggemtspeg
     1201 addgsspspd egivvsdraq pgatgssqtl akfrefllfg hkkdalewav kcglwghals
     1261 laskmdarty asimtrftsa latndplqtl yqhlsgrqpa avtciadekw gdwrphlami
     1321 lsnpssrpev darsittlgd vlasrgclsa ahfcylmaqv efgsysckss klvllgsshq
     1381 tllfeefasn eaiqcteiye ysqslanagy mlphlqaykf lhatrlaeyg faqealhyce
     1441 vlaeamgrqc gsaqlaaqty elasrlkyhd phfsqgpgel eelgeppwla afaqlvtqll
     1501 qqhaaaappq igalygqvss cggnavaevs ggdsyldqpg ftsstgfsmh dsgmsileqs
     1561 gnygnsyldp sacgdsaqav spsadqwtal ahtapdsrsq hlpptsvpsf aatgtsvesg
     1621 wtavqpsaey sfvstsppve smvnggqtdg tapssppvgs qtttfdyyka stysqaqhpp
     1681 hhqtsrerls svssstdahd rrdsvdsaal asgrsrlpsg pqsplttdqs asesertkpa
     1741 atnkgktqva gkswlggifg kllpkgpnqm ilpddkdpdi vwdehkkcwv dknaapgdad
     1801 iqsaapptds sfggkptpps gkpgenrfqm akqrslrkhy vdvlnpgssk asgdspavpg
     1861 vppvpeqpgt qsppqflvpq avddhcdsgy dfvspapmpd hsapappppp petvsyqqps
     1921 gkvvstpmmf dpadfssrav dsshkmaggn larrraypt
//
LOCUS       XP_077501127            1958 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X45 [Amblyomma
            americanum].
ACCESSION   XP_077501127
VERSION     XP_077501127.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645001.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1958
                     /product="uncharacterized protein LOC144111913 isoform
                     X45"
                     /calculated_mol_wt=212200
     Region          <349..656
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          619..>853
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1090..>1164
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1138..1502
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1260..1261,1263..1264,1266..1270,1272,1274,1284,
                     1286,1288..1289,1291..1292,1296,1302,1309,1318,1323,1326,
                     1354..1355)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1427,1449..1450,1452..1453,1456..1457,1460,
                     1489..1490,1493,1497)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1958
                     /gene="LOC144111913"
                     /coded_by="XM_077645001.1:93..5969"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqegwp savtgaangs tagsavhcgg paqgdnywnw aahqepgagg dtaasapmqs
      121 wnswgaaqev wtgraqppdp atvggtneaa vapvvndgts lppqlgytsp peggqqqygs
      181 ggvvaegalq egwaaphwsg dtnqwpsnle qqqfvtaqaq wqwqpadass avgqwqgygs
      241 qldpaaagpy estslagyap ldgslqqqqq qpgqdqlaas sgenvagdmh lvayssgpaa
      301 vdgqggsegt qhleatlgdn gmvsmffqdg gaddgqaesr eggptthdqh aeepgppshq
      361 qqtaatgiaq daisqvapaa ssdesvnqev lepvmrpepq dvlplqaegs ppageeavlv
      421 mpegqcpapa kkavirarqg gafkppalar qgssstetal mqqnaiqlhl ppenlellpp
      481 devgrhhasl rhrqgvaspa avgagltlld apemqpcvtl apaappiphd rpasrastgp
      541 qvapeatgce eeesvpdghc qtpvtrmvap plmrqsplgr eegtgrstaa papslrvatv
      601 lapsplladt tlpertepsg stdgdqpqpv vtaaragedk eglpvprpka tstptghcsa
      661 hheppnvsvv lahgkhdskd lgdkgsesht rqeepregkq ddrnsrdfrd srdfresrdp
      721 vhsrdyrdsr dagqargrgs dgesldwkgs qdnfehdssr vgrgdlrddq drkrdryyer
      781 addrreaske drdeyrkagr kigttedrkr kddyyddrra dqledrredr regfrddyrk
      841 tdrrgdryhd rrdqwkgqes ddewkerrsd rrsdrysrhd yryddrhsdr ysdrgrpssr
      901 tsqdayldey dqrvgdrrvr dnhnrpssrh sesdssspds rrgygtgkhr kyreadrapg
      961 vyydhqksyg taggydynay rrdyynyyhy gyaprsgygy gyydelyrtn paykqqvday
     1021 yarlgyspaq lmerlsvhsg rssangdsrr desfasnted dcdpsildne strldhvpqp
     1081 eevkkfsrph plarfcgang llklvptlsg gnlpppieih slkhlfqkdp qfhelkqfpg
     1141 plvrsqthkn dviqfcthki qsfaedpnlp drsshillwe llvlmlrqng fvsgsdiael
     1201 llrghnilep vvsasglarr ggeggemtsp egaddgssps pdegivvsdr aqpgatgssq
     1261 tlakfrefll fghkkdalew avkcglwgha lslaskmdar tyasimtrft salatndplq
     1321 tlyqhlsgrq paavtciade kwgdwrphla milsnpssrp evdarsittl gdvlasrgcl
     1381 saahfcylma qvefgsysck ssklvllgss hqtllfeefa sneaiqctei yeysqslana
     1441 gymlphlqay kflhatrlae ygfaqealhy cevlaeamgr qcgsaqlaaq tyelasrlky
     1501 hdphfsqgpg eleelgeppw laafaqlvtq llqqhaaaap pqigalygqv sscggnavae
     1561 vsggdsyldq pgftsstgfs mhdsgmsile qsgnygnsyl dpsacgdsaq avspsadqwt
     1621 alahtapdsr sqhlpptsvp sfaatgtsve sgwtavqpsa eysfvstspp vesmvnggqt
     1681 dgtapssppv gsqtttfdyy kastysaqhp phhqtsrerl ssvssstdah drrdsvdsaa
     1741 lasgrsrlps gpqsplttdq sasesertkp aatnkgktqi vwdehkkcwv dknaapgdad
     1801 iqsaapptds sfggkptpps gkpgenrfqm akqrslrkhy vdvlnpgssk asgdspavpg
     1861 vppvpeqpgt qsppqflvpq avddhcdsgy dfvspapmpd hsapappppp petvsyqpsg
     1921 kvvstpmmfd padfssravd sshkmaggnl arrraypt
//
LOCUS       XP_077501128            1953 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X46 [Amblyomma
            americanum].
ACCESSION   XP_077501128
VERSION     XP_077501128.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645002.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1953
                     /product="uncharacterized protein LOC144111913 isoform
                     X46"
                     /calculated_mol_wt=211649
     Region          <348..650
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          613..>847
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1084..>1158
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1132..1496
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1254..1255,1257..1258,1260..1264,1266,1268,1278,
                     1280,1282..1283,1285..1286,1290,1296,1303,1312,1317,1320,
                     1348..1349)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1421,1443..1444,1446..1447,1450..1451,1454,
                     1483..1484,1487,1491)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1953
                     /gene="LOC144111913"
                     /coded_by="XM_077645002.1:93..5954"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqegwp savtgaangs tagsavhcgg paqgdnywnw aahqepgagg dtaasapmqs
      121 wnswgaaqev wtgraqppdp atvggtneaa vapvvndgts lppqlgytsp peggqqqygs
      181 ggvvaegalq egwaaphwsg dtnqwpsnle qqqfvtaqaq wwqpadassa vgqwqgygsq
      241 ldpaaagpye stslagyapl dgslqqqqqq pgqdqlaass genvagdmhl vayssgpaav
      301 dgqggsegtq hleatlgdng mvsmffqdgg addgqaesre ggptthdqha eepgppshqq
      361 qtaatgiaqd aisqvapaas sdesvnqevl epvmrpepqd aegsppagee avlvmpegqc
      421 papakkavir arqggafkpp alarqgssst etalmqqnai qlhlppenle llppdevgrh
      481 haslrhrqgv aspaavgagl tlldapemqp cvtlapaapp iphdrpasra stgpqvapea
      541 tgceeeesvp dghcqtpvtr mvapplmrqs plgreegtgr staapapslr vatvlapspl
      601 ladttlpert epsgstdgdq pqpvvtaara gedkeglpvp rpkatstptg hcsahheppn
      661 vsvvlahgkh dskdlgdkgs eshtrqeepr egkqddrnsr dfrdsrdfre srdpvhsrdy
      721 rdsrdagqar grgsdgesld wkgsqdnfeh dssrvgrgdl rddqdrkrdr yyeraddrre
      781 askedrdeyr kagrkigtte drkrkddyyd drradqledr redrregfrd dyrktdrrgd
      841 ryhdrrdqwk gqesddewke rrsdrrsdry srhdyryddr hsdrysdrgr pssrtsqday
      901 ldeydqrvgd rrvrdnhnrp ssrhsesdss spdsrrgygt gkhrkyread rapgvyydhq
      961 ksygtaggyd ynayrrdyyn yyhygyaprs gygygyydel yrtnpaykqq vdayyarlgy
     1021 spaqlmerls vhsgrssang dsrrdesfas nteddcdpsi ldnestrldh vpqpeevkkf
     1081 srphplarfc gangllklvp tlsggnlppp ieihslkhlf qkdpqfhelk qfpgplvrsq
     1141 thkndviqfc thkiqsfaed pnlpdrsshi llwellvlml rqngfvsgsd iaelllrghn
     1201 ilepvvsasg larrggegge mtspegaddg sspspdegiv vsdraqpgat gssqtlakfr
     1261 efllfghkkd alewavkcgl wghalslask mdartyasim trftsalatn dplqtlyqhl
     1321 sgrqpaavtc iadekwgdwr phlamilsnp ssrpevdars ittlgdvlas rgclsaahfc
     1381 ylmaqvefgs ysckssklvl lgsshqtllf eefasneaiq cteiyeysqs lanagymlph
     1441 lqaykflhat rlaeygfaqe alhycevlae amgrqcgsaq laaqtyelas rlkyhdphfs
     1501 qgpgeleelg eppwlaafaq lvtqllqqha aaappqigal ygqvsscggn avaevsggds
     1561 yldqpgftss tgfsmhdsgm sileqsgnyg nsyldpsacg dsaqavspsa dqwtalahta
     1621 pdsrsqhlpp tsvpsfaatg tsvesgwtav qpsaeysfvs tsppvesmvn ggqtdgtaps
     1681 sppvgsqttt fdyykastys aqhpphhqts rerlssvsss tdahdrrdsv dsaalasgrs
     1741 rlpsgpqspl ttdqsasese rtkpaatnkg ktqivwdehk kcwvdknaap gdadiqsaap
     1801 ptdssfggkp tppsgkpgen rfqmakqrsl rkhyvdvlnp gsskasgdsp avpgvppvpe
     1861 qpgtqsppqf lvpqavddhc dsgydfvspa pmpdhsapap pppppetvsy qqpsgkvvst
     1921 pmmfdpadfs sravdsshkm aggnlarrra ypt
//
LOCUS       XP_077501129            1952 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111913 isoform X47 [Amblyomma
            americanum].
ACCESSION   XP_077501129
VERSION     XP_077501129.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645003.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1952
                     /product="uncharacterized protein LOC144111913 isoform
                     X47"
                     /calculated_mol_wt=211852
     Region          <311..618
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          581..>815
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1052..>1126
                     /region_name="Sec16"
                     /note="Vesicle coat trafficking protein Sec16 mid-region;
                     pfam12932"
                     /db_xref="CDD:432885"
     Region          1100..1464
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cd09233"
                     /db_xref="CDD:187750"
     Site            order(1222..1223,1225..1226,1228..1232,1234,1236,1246,
                     1248,1250..1251,1253..1254,1258,1264,1271,1280,1285,1288,
                     1316..1317)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Site            order(1389,1411..1412,1414..1415,1418..1419,1422,
                     1451..1452,1455,1459)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     CDS             1..1952
                     /gene="LOC144111913"
                     /coded_by="XM_077645003.1:93..5951"
                     /db_xref="GeneID:144111913"
ORIGIN      
        1 msrrmlqrry ptgkaaeaaq paktlpqpvg evmpqwdgst sfqptqypaa lldpqqqqrq
       61 qqsnlqepga ggdtaasapm qswnswgaaq evwtgraqpp dpatvggtne aavapvvndg
      121 tslppqlgyt sppeggqqqy gsggvvaega lqegwaaphw sgdtnqwpsn leqqqfvtaq
      181 aqwqwqpada ssavgqwqgy gsqldpaaag pyestslagy apldgslqqq qqqpgqdqla
      241 assgenvagd mhlvayssgp aavdgqggse gtqhleatlg dngmvsmffq dggaddgqae
      301 sreggptthd qhaeepgpps hqqqtaatgi aqdaisqvap aassdesvnq evlepvmrpe
      361 pqdvlplqae gsppageeav lvmpegqcpa pakkavirar qggafkppal arqgssstet
      421 almqqnaiql hlppenlell ppdevgrhha slrhrqgvas paavgagltl ldapemqpcv
      481 tlapaappip hdrpasrast gpqvapeatg ceeeesvpdg hcqtpvtrmv applmrqspl
      541 greegtgrst aapapslrva tvlapsplla dttlpertep sgstdgdqpq pvvtaarage
      601 dkeglpvprp katstptghc sahheppnvs vvlahgkhds kdlgdkgses htrqeepreg
      661 kqddrnsrdf rdsrdfresr dpvhsrdyrd srdagqargr gsdgesldwk gsqdnfehds
      721 srvgrgdlrd dqdrkrdryy eraddrreas kedrdeyrka grkigttedr krkddyyddr
      781 radqledrre drregfrddy rktdrrgdry hdrrdqwkgq esddewkerr sdrrsdrysr
      841 hdyryddrhs drysdrgrps srtsqdayld eydqrvgdrr vrdnhnrpss rhsesdsssp
      901 dsrrgygtgk hrkyreadra pgvyydhqks ygtaggydyn ayrrdyynyy hygyaprsgy
      961 gygyydelyr tnpaykqqvd ayyarlgysp aqlmerlsvh sgrssangds rrdesfasnt
     1021 eddcdpsild nestrldhvp qpeevkkfsr phplarfcga ngllklvptl sggnlpppie
     1081 ihslkhlfqk dpqfhelkqf pgplvrsqth kndviqfcth kiqsfaedpn lpdrsshill
     1141 wellvlmlrq ngfvsgsdia elllrghnil epvvsasgla rrggeggemt spegaddgss
     1201 pspdegivvs draqpgatgs sqtlakfref llfghkkdal ewavkcglwg halslaskmd
     1261 artyasimtr ftsalatndp lqtlyqhlsg rqpaavtcia dekwgdwrph lamilsnpss
     1321 rpevdarsit tlgdvlasrg clsaahfcyl maqvefgsys ckssklvllg sshqtllfee
     1381 fasneaiqct eiyeysqsla nagymlphlq aykflhatrl aeygfaqeal hycevlaeam
     1441 grqcgsaqla aqtyelasrl kyhdphfsqg pgeleelgep pwlaafaqlv tqllqqhaaa
     1501 appqigalyg qvsscggnav aevsggdsyl dqpgftsstg fsmhdsgmsi leqsgnygns
     1561 yldpsacgds aqavspsadq wtalahtapd srsqhlppts vpsfaatgts vesgwtavqp
     1621 saeysfvsts ppvesmvngg qtdgtapssp pvgsqtttfd yykastysaq hpphhqtsre
     1681 rlssvssstd ahdrrdsvds aalasgrsrl psgpqspltt dqsasesert kpaatnkgkt
     1741 qvagkswlgg ifgkllpkgp nqmilpddkd pdivwdehkk cwvdknaapg dadiqsaapp
     1801 tdssfggkpt ppsgkpgenr fqmakqrslr khyvdvlnpg sskasgdspa vpgvppvpeq
     1861 pgtqsppqfl vpqavddhcd sgydfvspap mpdhsapapp ppppetvsyq qpsgkvvstp
     1921 mmfdpadfss ravdsshkma ggnlarrray pt
//
LOCUS       XP_077501130             716 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111918 [Amblyomma americanum].
ACCESSION   XP_077501130
VERSION     XP_077501130.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645004.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..716
                     /product="uncharacterized protein LOC144111918"
                     /calculated_mol_wt=79154
     CDS             1..716
                     /gene="LOC144111918"
                     /coded_by="XM_077645004.1:141..2291"
                     /db_xref="GeneID:144111918"
ORIGIN      
        1 mlllrlsaar alrendslrf nsccpfcifv nssaacgfvr epamerrtgq kyrtkyaygk
       61 rkrkppipkq krpaateshe rrsapissha dvsseaslpl esatssesgg lapsashdia
      121 vqyrpghssv faaasddavp vqrddgevfp kqkrtvqvhl eprfvtleaa eeqacsvrat
      181 lravssterk fgfarqteee sapdedeftl iqasvmnsii gsllcpkcsm krlsvkhetk
      241 lglavklvls cgacgpiash wsskrksegr tfevniramq aiktigkgpt alndfwatmn
      301 vshrglhqkt yqghlkklfk pgaeeaaqni fadavlavkd vyskmqpssp snitvvydgt
      361 wltrghsshi gvgciiefyt glvldctvls nfclgccqqp aesdpsygtw ieqhqcqknt
      421 dvasgqmeve aalilfrrsf ssyglrytnv icdgdsrtyl alckdevygf ipltkedcin
      481 hvqkrmgtal rtlvarakkg eplggkgglt qdlikkltny yglalrrnte vsdmqravma
      541 tlyhvtstdd ephhelcppg pdswcrhrsa qakmepppph kyklprrvae allpvyqrls
      601 dpqllecckg nktqnaaesl hsviwsmisk dqhaslfave tavheavary nfgnlrayte
      661 vcksvgikpg slaleraqek dkqrkrkacs aektkeqrhk ktpaskdtky yspgaf
//
LOCUS       XP_077501132             447 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111919 [Amblyomma americanum].
ACCESSION   XP_077501132
VERSION     XP_077501132.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645006.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 37% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..447
                     /product="uncharacterized protein LOC144111919"
                     /calculated_mol_wt=49301
     Region          73..93
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          99..121
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          101..121
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(106,108,110,112..113,116..117,120,134,136,140..141,
                     144..145,148,162,164,166,168..169,172..173,176)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          113..138
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          129..149
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          142..166
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          155..179
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          157..177
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          169..194
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          185..205
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          197..222
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          213..233
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          241..261
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          <426..443
                     /region_name="CLAMP"
                     /note="Flagellar C1a complex subunit C1a-32; pfam14769"
                     /db_xref="CDD:464306"
     CDS             1..447
                     /gene="LOC144111919"
                     /coded_by="XM_077645006.1:331..1674"
                     /db_xref="GeneID:144111919"
ORIGIN      
        1 mdfqqepnpq vkndvasnpe yppassvlqs pstsrpgcvm hgnwkyqgss matneensvq
       61 yqnpgitgep lrcphcpyts tkhadvkrhi nthapgekfk cplclkafat ssnlkrhmss
      121 htgekpykce nclfaavtns qlvrhrrmht gdkpykcdnc pfsaatsgdl vrhmrrhtge
      181 rpykcdrcpy aaarsdhllw hmrghtgekp hkcevcsyst iqkghldrhm lthksaegfh
      241 cslcslvfss iqslkqhisg hrldgcvlty arvatgtapp hddgsellmd qeelenagap
      301 ppsglsdgpa feqpelsagn eksqgdptae ttgkkadaaa ppeeptkgst tgdhdseepm
      361 dttvasikrs rdfekapatd relrrlerhc igdqlvgtap tesisfanat taslcltrsi
      421 lvpqtthtyf rhfrfyhfsf fpqadds
//
LOCUS       XP_077501133             341 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111920 [Amblyomma americanum].
ACCESSION   XP_077501133
VERSION     XP_077501133.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645007.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..341
                     /product="uncharacterized protein LOC144111920"
                     /calculated_mol_wt=35144
     CDS             1..341
                     /gene="LOC144111920"
                     /coded_by="XM_077645007.1:38..1063"
                     /db_xref="GeneID:144111920"
ORIGIN      
        1 mlwahlnifs ptsarmaawc sssrlraskt serlvgpgpg lsstspesss issdskskak
       61 assgakygrs gpddtaddcs gihgaeesgk csvgdgqgsr prrvpaesqy dstwkantgv
      121 nivqesvscp aeltlcrrln tvasghvidd vvdgggfqak assgakygrs gpddtaddcs
      181 gihgaeesgk csvgdgqgsr prrvpaesqy dstwkantgv nivqesvscp aeltlcrrln
      241 tvasghvidd vvdgggfqak assgakygrs gpddtaddcs gihgaeesgk csvgdgqgsr
      301 prrvpaesqy dstwkantgv nivqesvscp fdtlfivpii a
//
LOCUS       XP_077501134             398 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111922 [Amblyomma americanum].
ACCESSION   XP_077501134
VERSION     XP_077501134.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645008.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526485) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..398
                     /product="uncharacterized protein LOC144111922"
                     /calculated_mol_wt=44599
     Region          169..388
                     /region_name="COG4372"
                     /note="Uncharacterized protein, contains DUF3084 domain
                     [Function unknown]"
                     /db_xref="CDD:443500"
     CDS             1..398
                     /gene="LOC144111922"
                     /coded_by="XM_077645008.1:299..1495"
                     /db_xref="GeneID:144111922"
ORIGIN      
        1 mstdvplfvq vltgffsial gravsadsgf nrhpasadaf rlpcaggvhf shcellswhv
       61 dttfcapsss tatsstlpar ilpikgtpap sissghgnpv vtmstdvplf vqvggrkycc
      121 rsnclflivl pcprtllvcf sdcisivgll lklsgdveen pgpdtmemiq qviasqteil
      181 sklseiqenq tssearildm qsrlsaieqk lqtfdesrdr lskletfaer ceiemttvsr
      241 kldelenrsr rnnlivrgvk eeeseseedl lqkvnndifd kilkqrvdti erihrlgkre
      301 pgrdrpiiik ltdcrdkmki msncfnlkgt qfsvnedfsk rvveirknlw nscaderkng
      361 vkaklifdkl kvkntlyawn eetkqrfkcq vatstsne
//
LOCUS       XP_077501135             251 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111923 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501135
VERSION     XP_077501135.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645009.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526487) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..251
                     /product="uncharacterized protein LOC144111923 isoform X1"
                     /calculated_mol_wt=26972
     CDS             1..251
                     /gene="LOC144111923"
                     /coded_by="XM_077645009.1:563..1318"
                     /db_xref="GeneID:144111923"
ORIGIN      
        1 mlgqpkqksk eedarkkrdr hktgggsaqc tvsaqseqvi avashimtrv gnrtdsdggi
       61 nlppvaslpv irllqpmvdg agneefhyae ddwepplvde pqspaaafgk ggttpeaveq
      121 esnlfspavq padasstaaa gcssatvasg aaagpttaea vatgdagrap rgrmalfers
      181 ladendyraa llreehglcl qlmredhnsi mqerqekkll diqkkklele ileiekqikl
      241 eelrrlreap e
//
LOCUS       XP_077501137             161 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111923 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501137
VERSION     XP_077501137.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645011.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526487) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..161
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..161
                     /product="uncharacterized protein LOC144111923 isoform X2"
                     /calculated_mol_wt=17929
     CDS             1..161
                     /gene="LOC144111923"
                     /coded_by="XM_077645011.1:563..1048"
                     /db_xref="GeneID:144111923"
ORIGIN      
        1 mlgqpkqksk eedarkkrdr hktgggsaqc tvsaqseqvi avashimtrk ttgnlrswms
       61 rshqrlllvr vgqlqkqlsr raisflllcn llmlpaqlqq aaavlqllle lplvplqlkq
      121 wppvtlaglq eggwpfskgl wqmrttierp ycaksmdcvc s
//
LOCUS       XP_077501139             486 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111926 [Amblyomma americanum].
ACCESSION   XP_077501139
VERSION     XP_077501139.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645013.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526487) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 28% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..486
                     /product="uncharacterized protein LOC144111926"
                     /calculated_mol_wt=54701
     CDS             1..486
                     /gene="LOC144111926"
                     /coded_by="XM_077645013.1:1..1461"
                     /db_xref="GeneID:144111926"
ORIGIN      
        1 mdatyvpssr ktlggckkrv qkatdtavty lgeatlpsvf ddilkrgpkf sleprsrkhe
       61 llavvhrvad kapfeekarc vsegvdclmr twtgrrqpsr dlrplvahfq qeglsllqad
      121 keasadafrl pcaggvhfsh cellswhvdt tfcapsssta tsstlparil pikgtpapsi
      181 ssghgnpvvt mstdvplsvq vggrkyccrs nclflivlpc prtllvcfsd cisivglllk
      241 lsgdveenpg pdtmemiqqv iasqteilsk lsemqenqts searildmqs rlsaieqklq
      301 tfdesrdrls kletfaerce iemttvsrkl delenrsrrn nlivrgvkee eseseedllq
      361 kvnndifdki lkqrvdtier ihrlgkrepg rdrpiiiklt dfrdkmkims ncfnlkgtqf
      421 svnedfskrv veirknlwns caderkngvk aklifdklki kntlyawnee tkqrfkcqva
      481 tstsne
//
LOCUS       XP_077501140             390 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111927 [Amblyomma americanum].
ACCESSION   XP_077501140
VERSION     XP_077501140.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645014.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526488) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..390
                     /product="uncharacterized protein LOC144111927"
                     /calculated_mol_wt=43035
     Region          164..>257
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     Region          <169..>368
                     /region_name="COG4372"
                     /note="Uncharacterized protein, contains DUF3084 domain
                     [Function unknown]"
                     /db_xref="CDD:443500"
     CDS             1..390
                     /gene="LOC144111927"
                     /coded_by="XM_077645014.1:277..1449"
                     /db_xref="GeneID:144111927"
ORIGIN      
        1 mqvfaqiiaf aflpvltarp pgivpgiwtp hqegeicarh pvatdtwepl atpplenwtk
       61 qaasaapssp tapfasaikq rhplsahcgl cgcatvrtan pltfvmqvgv pytlfakkss
      121 nyclvqlpsp qcclclavec vnvihallll sgdvetnpgs pntdavlael qklsagqrkl
      181 isevqglknq lvttdktisa lserianlet qfevistlrt eietlqtsta rtthqlseid
      241 trlddlenrs rrnnlvfygl pdpnekesyd qsekiiiqhc ldslnlvidp kdierahrlg
      301 rhsidhqrpi ivklsafktk gvilsngpkl kntdfsiged fsrsvrnark hlvafartks
      361 gpfslryktl hmgpkrymfd qdtqtvkeia
//
LOCUS       XP_077501141             355 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111930, partial [Amblyomma
            americanum].
ACCESSION   XP_077501141
VERSION     XP_077501141.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645015.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526490) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         <1..355
                     /product="uncharacterized protein LOC144111930"
     Region          93..138
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          173..340
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..355
                     /gene="LOC144111930"
                     /coded_by="XM_077645015.1:<1..1068"
                     /db_xref="GeneID:144111930"
ORIGIN      
        1 etdlsmqnis hlieengrlk qrlasleeec saakqkaael enkhtnhaii tkevvmqsak
       61 tvkfytgfvs tamftaflqf llsawkpsca twldaetqli mvlmrlrlgl ltqdlscrfg
      121 isngtvssif hawldvlaan mtkwiiwpsr qtlqanrpaa fqdpffdgvt giidctevfi
      181 qrpilmtars qtyshykhhn tvkilvvisp sgaitfvska wggrtpdkei tlrsglldkv
      241 kegdvflvdr gfrcgemfaa rgatllmpsl tknraqlpga evttsrkvss vrihverair
      301 rlkvfrifqt vlplnfvkrt gdknlatidk vvvvcsalvn lqtpiiqnsq rrsas
//
LOCUS       XP_077501142             696 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 1-like isoform
            X1 [Amblyomma americanum].
ACCESSION   XP_077501142
VERSION     XP_077501142.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645016.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..696
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 1-like isoform X1"
                     /calculated_mol_wt=79763
     Region          <23..82
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          23..50
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(24..25,28..30,32..33,37,40,50,52,54,58..59,62..64,
                     66..67,71,74,83,85,87,91,94,96..97,101,104)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          52..83
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          113..229
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          278..393
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          392..>447
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     Region          <464..617
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..696
                     /gene="LOC144111931"
                     /coded_by="XM_077645016.1:116..2206"
                     /db_xref="GeneID:144111931"
ORIGIN      
        1 mrlekdalia eshdglrrnt lelflscrtg dlarvkylve eqeselnvrd rwdgtplyya
       61 clcghkdvve yllfqgascv antfdgercl yaslsleird llrdrkvits ntmrrdtyde
      121 flrrcledsq hcdvsfsvqg eiipahrcvl aarcdffrrs lldkwagrql ipvthhavdg
      181 avfriitqyl ytgrlemqak hfesflelas rcqlisliad vkealaevln qgsqrsgvsa
      241 esetillept hyreqlqrdf stlpvelasg vtaggaafpa eehnhadici nvdgthflch
      301 kvflcnrsey fraliedhft easrlgsdqq qlpvielqqv tpnifgcvlr hvysnsddrv
      361 natnvwdvlc aadvyllpdl krqcgaqiar lldvesicgt lrasrlfrlp rlenqciefm
      421 akhlaeviel pefheaired akevklrsfl kthfyyyfyp fyhavcyftg citffavasf
      481 krskmpkvlq atawciavac gmccifmktv wyrtrdptte fgklsaaffd rilwsiflaw
      541 itlscatgrg gflnkflsws aftplsrlaf gvyiihlpfi hlclhisrer iffshfsvvs
      601 fffsmlvwsy llsyllfifc eaptgrldkl ifdpqrrkte grekdeapnd nsvqdklpda
      661 vvtfrdrkld iwqtsseknt sngilpgncs rtschl
//
LOCUS       XP_077501143             638 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 1-like isoform
            X2 [Amblyomma americanum].
ACCESSION   XP_077501143
VERSION     XP_077501143.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645017.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..638
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 1-like isoform X2"
                     /calculated_mol_wt=73024
     Region          <23..>106
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          23..50
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(24..25,28..30,32..33,37,40,50,52,54,58..59,62..64,
                     66..67,71,74,83,85,87,91,94,96..97,101,104)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          52..83
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          113..229
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          278..335
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          334..>389
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     Region          <406..559
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..638
                     /gene="LOC144111931"
                     /coded_by="XM_077645017.1:116..2032"
                     /db_xref="GeneID:144111931"
ORIGIN      
        1 mrlekdalia eshdglrrnt lelflscrtg dlarvkylve eqeselnvrd rwdgtplyya
       61 clcghkdvve yllfqgascv antfdgercl yaslsleird llrdrkvits ntmrrdtyde
      121 flrrcledsq hcdvsfsvqg eiipahrcvl aarcdffrrs lldkwagrql ipvthhavdg
      181 avfriitqyl ytgrlemqak hfesflelas rcqlisliad vkealaevln qgsqrsgvsa
      241 esetillept hyreqlqrdf stlpvelasg vtaggaafpa eehnhadici nvdgthflch
      301 kvnatnvwdv lcaadvyllp dlkrqcgaqi arlldvesic gtlrasrlfr lprlenqcie
      361 fmakhlaevi elpefheair edakevklrs flkthfyyyf ypfyhavcyf tgcitffava
      421 sfkrskmpkv lqatawciav acgmccifmk tvwyrtrdpt tefgklsaaf fdrilwsifl
      481 awitlscatg rggflnkfls wsaftplsrl afgvyiihlp fihlclhisr eriffshfsv
      541 vsfffsmlvw syllsyllfi fceaptgrld klifdpqrrk tegrekdeap ndnsvqdklp
      601 davvtfrdrk ldiwqtssek ntsngilpgn csrtschl
//
LOCUS       XP_077501144             584 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 1-like isoform
            X3 [Amblyomma americanum].
ACCESSION   XP_077501144
VERSION     XP_077501144.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645018.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..584
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 1-like isoform X3"
                     /calculated_mol_wt=66904
     Region          1..117
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          166..281
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          280..>335
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     Region          <352..505
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..584
                     /gene="LOC144111931"
                     /coded_by="XM_077645018.1:330..2084"
                     /db_xref="GeneID:144111931"
ORIGIN      
        1 mrrdtydefl rrcledsqhc dvsfsvqgei ipahrcvlaa rcdffrrsll dkwagrqlip
       61 vthhavdgav friitqylyt grlemqakhf esflelasrc qlisliadvk ealaevlnqg
      121 sqrsgvsaes etillepthy reqlqrdfst lpvelasgvt aggaafpaee hnhadicinv
      181 dgthflchkv flcnrseyfr aliedhftea srlgsdqqql pvielqqvtp nifgcvlrhv
      241 ysnsddrvna tnvwdvlcaa dvyllpdlkr qcgaqiarll dvesicgtlr asrlfrlprl
      301 enqciefmak hlaevielpe fheairedak evklrsflkt hfyyyfypfy havcyftgci
      361 tffavasfkr skmpkvlqat awciavacgm ccifmktvwy rtrdpttefg klsaaffdri
      421 lwsiflawit lscatgrggf lnkflswsaf tplsrlafgv yiihlpfihl clhisrerif
      481 fshfsvvsff fsmlvwsyll syllfifcea ptgrldklif dpqrrktegr ekdeapndns
      541 vqdklpdavv tfrdrkldiw qtssekntsn gilpgncsrt schl
//
LOCUS       XP_077501146             696 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 1-like isoform
            X1 [Amblyomma americanum].
ACCESSION   XP_077501146
VERSION     XP_077501146.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645020.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..696
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 1-like isoform X1"
                     /calculated_mol_wt=79703
     Region          <23..>106
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          23..50
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(24..25,28..30,32..33,37,40,50,52,54,58..59,62..64,
                     66..67,71,74,83,85,87,91,94,96..97,101,104)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          52..83
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          113..229
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          278..393
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          392..>447
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     Region          <464..617
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..696
                     /gene="LOC144111932"
                     /coded_by="XM_077645020.1:116..2206"
                     /db_xref="GeneID:144111932"
ORIGIN      
        1 mrlekdalia eshdglrrnt lelflscrtg dlarvkylve eqeselnvrd rwdgtplyya
       61 clcghkdvve yllsqgascv antfdgercl yaslsleird llrdrkvits ntmrrdtyde
      121 flrrcledsq hcdvsfsvqg eiipahrcvl aarcdffrrs lldkwagrql ipvthhavdg
      181 avfriitqyl ytgrlemqak hfesflelas rcqlisliad vkealaevln qgsqrsgvsa
      241 esetillept hyreqlqrdf stlpvelasg vtaggaafpa eehnhadici nvdgthflch
      301 kvflcnrsey fraliedhft easrlgsdqq qlpvielqqv tpnifgcvlr hvysnsddrv
      361 natnvwdvlc aadvyllpdl krqcgaqiar lldvesicgt lrasrlfrlp rlenqciefm
      421 akhlaeviel pefheaired akevklrsfl kthfyyyfyp fyhavcyftg citffavasf
      481 krskmpkvlq atawciavac gmccifmktv wyrtrdptte fgklsaaffd rilwsiflaw
      541 itlscatgrg gflnkflsws aftplsrlaf gvyiihlpfi hlclhisrer iffshfsvvs
      601 fffsmlvwsy llsyllfifc eaptgrldkl ifdpqrrkte grekdeapnd nsvqdklpda
      661 vvtfrdrkld iwqtsseknt sngilpgncs rtschl
//
LOCUS       XP_077501147             638 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 1-like isoform
            X2 [Amblyomma americanum].
ACCESSION   XP_077501147
VERSION     XP_077501147.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645021.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..638
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 1-like isoform X2"
                     /calculated_mol_wt=72964
     Region          <23..>106
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          23..50
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(24..25,28..30,32..33,37,40,50,52,54,58..59,62..64,
                     66..67,71,74,83,85,87,91,94,96..97,101,104)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          52..83
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          113..229
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          278..335
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          334..>389
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     Region          <406..559
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..638
                     /gene="LOC144111932"
                     /coded_by="XM_077645021.1:116..2032"
                     /db_xref="GeneID:144111932"
ORIGIN      
        1 mrlekdalia eshdglrrnt lelflscrtg dlarvkylve eqeselnvrd rwdgtplyya
       61 clcghkdvve yllsqgascv antfdgercl yaslsleird llrdrkvits ntmrrdtyde
      121 flrrcledsq hcdvsfsvqg eiipahrcvl aarcdffrrs lldkwagrql ipvthhavdg
      181 avfriitqyl ytgrlemqak hfesflelas rcqlisliad vkealaevln qgsqrsgvsa
      241 esetillept hyreqlqrdf stlpvelasg vtaggaafpa eehnhadici nvdgthflch
      301 kvnatnvwdv lcaadvyllp dlkrqcgaqi arlldvesic gtlrasrlfr lprlenqcie
      361 fmakhlaevi elpefheair edakevklrs flkthfyyyf ypfyhavcyf tgcitffava
      421 sfkrskmpkv lqatawciav acgmccifmk tvwyrtrdpt tefgklsaaf fdrilwsifl
      481 awitlscatg rggflnkfls wsaftplsrl afgvyiihlp fihlclhisr eriffshfsv
      541 vsfffsmlvw syllsyllfi fceaptgrld klifdpqrrk tegrekdeap ndnsvqdklp
      601 davvtfrdrk ldiwqtssek ntsngilpgn csrtschl
//
LOCUS       XP_077501148             584 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 1-like isoform
            X3 [Amblyomma americanum].
ACCESSION   XP_077501148
VERSION     XP_077501148.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645022.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..584
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 1-like isoform X3"
                     /calculated_mol_wt=66904
     Region          1..117
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          166..281
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          280..>335
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     Region          <352..505
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..584
                     /gene="LOC144111932"
                     /coded_by="XM_077645022.1:332..2086"
                     /db_xref="GeneID:144111932"
ORIGIN      
        1 mrrdtydefl rrcledsqhc dvsfsvqgei ipahrcvlaa rcdffrrsll dkwagrqlip
       61 vthhavdgav friitqylyt grlemqakhf esflelasrc qlisliadvk ealaevlnqg
      121 sqrsgvsaes etillepthy reqlqrdfst lpvelasgvt aggaafpaee hnhadicinv
      181 dgthflchkv flcnrseyfr aliedhftea srlgsdqqql pvielqqvtp nifgcvlrhv
      241 ysnsddrvna tnvwdvlcaa dvyllpdlkr qcgaqiarll dvesicgtlr asrlfrlprl
      301 enqciefmak hlaevielpe fheairedak evklrsflkt hfyyyfypfy havcyftgci
      361 tffavasfkr skmpkvlqat awciavacgm ccifmktvwy rtrdpttefg klsaaffdri
      421 lwsiflawit lscatgrggf lnkflswsaf tplsrlafgv yiihlpfihl clhisrerif
      481 fshfsvvsff fsmlvwsyll syllfifcea ptgrldklif dpqrrktegr ekdeapndns
      541 vqdklpdavv tfrdrkldiw qtssekntsn gilpgncsrt schl
//
LOCUS       XP_077501149             149 aa            linear   INV 25-NOV-2025
DEFINITION  N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D-like
            [Amblyomma americanum].
ACCESSION   XP_077501149
VERSION     XP_077501149.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645023.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..149
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..149
                     /product="N-acyl-phosphatidylethanolamine-hydrolyzing
                     phospholipase D-like"
                     /calculated_mol_wt=17038
     Region          <16..120
                     /region_name="UlaG"
                     /note="L-ascorbate lactonase UlaG, metallo-beta-lactamase
                     superfamily [Carbohydrate transport and metabolism];
                     COG2220"
                     /db_xref="CDD:441822"
     Region          <16..66
                     /region_name="metallo-hydrolase-like_MBL-fold"
                     /note="mainly hydrolytic enzymes and related proteins
                     which carry out various biological functions; MBL-fold
                     metallohydrolase domain; cl23716"
                     /db_xref="CDD:451500"
     CDS             1..149
                     /gene="LOC144111933"
                     /coded_by="XM_077645023.1:152..601"
                     /db_xref="GeneID:144111933"
ORIGIN      
        1 mltdylgvil fllqvlwggw cvispeyrfy fagdtgycdv fkqigqaygp fdvaaipiga
       61 yeprwfmkyq hvnpeeavli hkdvrsratl avhwgtftla neyyldppvk lresldrhgi
      121 sprefftlkh gesrllhatt snrtsrvlp
//
LOCUS       XP_077501151            1914 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111934 [Amblyomma americanum].
ACCESSION   XP_077501151
VERSION     XP_077501151.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645025.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1914
                     /product="uncharacterized protein LOC144111934"
                     /calculated_mol_wt=207931
     Region          <523..922
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          <889..1148
                     /region_name="rne"
                     /note="ribonuclease E; Reviewed; PRK10811"
                     /db_xref="CDD:236766"
     CDS             1..1914
                     /gene="LOC144111934"
                     /coded_by="XM_077645025.1:144..5888"
                     /db_xref="GeneID:144111934"
ORIGIN      
        1 madedplneh cewkencsra liyvrtwnqa aseaprdqgg qrlkrrcarl pskhspakil
       61 rldqatgtet segsmtapqg hkhptahgdg asdersmlin mraemgdrrs ltplppspph
      121 ggeeskvdqf evtlpdlpsi slwlpwslqs vasppepets spepvpestp ppgpsqptrl
      181 leacvqvsre eqlepatlee pvvplasfel pqdaaegqeh gkeqmafasi svpepglvle
      241 vtseppqpkr lseahmqesl deqqdlamrd vkelvalsvs sklphetagm lehvndtmal
      301 asvsapdlrp vpeltptsep pqptqpsgas vqeyleeqle pftsskleqq nlameaarel
      361 mvtsgsfelp qetamqleha eeamapvais alnpgllpev splseasesr hvlvastqas
      421 lseelksaav skmelqapvm kaveeltmts assklpqdia gmlehvagpm algaisvqet
      481 gldvparsep pqtawppdlf mlsqvaesaa rcelkqqnlg qegpvtalpa spelapasvl
      541 esvalaasse pvqatttall pqlaesglsp hpeavfeser rpwvkrrirr plsehspati
      601 swpdqttgte tpegsmtatq ghkhptahgr pwlkrrirhl psehspatis rldqatgtet
      661 pegsmtvtqg hkhptahgdg asdersmlln miaemgdprs ltplppspph ggeeskvdqf
      721 evtlpelpsk spwlawspls vaspsepets ppepvpestp ppessqptrl leacvqvsre
      781 eqlepatlee pvvplasfel pqdaaegqeh gkeqtafapi sipepglvle vtseppqpmr
      841 lseahmqesl deqqdfemrd vkelvalsvs sklphetagm lepvndtmal asvsapdlrp
      901 vpeltptsep pqptqpswds vqeyleeqle pftsskreqq nlameaarel mvtsgsfelp
      961 qetamqleha eeamapvsis alkpgllpev splseasepr hvlvastqas lseelksaav
     1021 skmelqapvm eaveeltmts assklpqdia gmlehvaepm algaisvqet gldvlvrsep
     1081 pqtawppdlf mlsqvaesaa rcelkqenlg qeapvtalpa spelapapvl esvalaasse
     1141 pvqptttall pqlaesglsp hpeaafesep hpaaaaspae qrdlgaelpv vcpmtvrtsa
     1201 aatltasgtg qlregtqpae aahlpsrarp sreessseda eqsattgqec plqpqqmgsv
     1261 laalmaivek lgqisdnmql frnelglwfr pdlhhptelr drrcascgqe qssgvapllv
     1321 rgqevpgqeq hshdemhngr plevalptkr rhsvhqmseg gpfngleeee evcrkrsrpe
     1381 ivsltspltp phsvperlsr easpipaprl skhcrlprpv evqetspipa prlskdsrlp
     1441 rpvevqeasp tlasqltdns clprpvevqe asptlapqlt enscllrpve vqeasptpas
     1501 qlndnsclpr pvevqeaspt lvprlieksc lprpvevqeg kreeafaymq flqspahaql
     1561 gelalseisl skevrafldq lrtlaslseq sdsvavlvsn tehntflcav ltllsrcktn
     1621 livefcqgkc kaplftkmen llliamahaa pdrqaallfv liarfcsiwq pksllpqaay
     1681 vrfmfalcrs lgeravardm iwelmrsdra sflvasavga wpglfdalsp gpvrnvisyv
     1741 lhrcpsnels dtllsycktv ldklgplpcf dgnlyplvdd llhplltthe cmdtclelrk
     1801 aleeicrqsp nsirpwvlrk qllpllqsll sklqlplaqq hatclirllg plweeskkfp
     1861 kgldplleqm adclahcgsp lrgaiieafk rlpgfqksrw paelsywflh wnrs
//
LOCUS       XP_077501152             759 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077501152
VERSION     XP_077501152.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645026.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..759
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=85589
     Region          76..202
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          296..701
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..759
                     /gene="LOC144111935"
                     /coded_by="XM_077645026.1:260..2539"
                     /db_xref="GeneID:144111935"
ORIGIN      
        1 msvqkvvliv vtvllcgclp cclggsgsdg dssasmteqq nvsyvsklke wiasamnras
       61 psltrkllea evsadcsvgl ltlvrgvrnl epwalrlida takypsgala ttrvdlgafd
      121 ecvqtvvrne yghetaraqy cnvvayggnk tdlddliaaa msithprvak frghiyeqri
      181 pimrlgicvl dvcnqdelql makavlpkvl dvtitdcvta lapdmtrtqv iilaflgtlv
      241 llivlgtavd vycsrrkqkg enkgvllsvl tsfsvvsntr mmlqittnks sdaysmrflh
      301 girffsivyi lvghsygaps diwanmvnai vyadnwanif ttvgymsvdc ffflngflla
      361 fvvhrqkrng vivfviavvr rfirtmvpvf fclmclfllp litsgpdaka yfrklndefq
      421 rqwlylllqv qnydfevtvd tpifghlwyl fldfqfflvs lpillllksr priaaaafvl
      481 lsfvgcsvat wqvagnemtp fivpltesit tflrtgfhyy fypfyhavcy fvgcmtyffv
      541 gifkekkisk acqfaawlfa iacglfcifi kvpwyqvkdp ttefgklsla ffdrilwsil
      601 mswvtiacat grggflntfl swsfftplsr lafgvyiihv pfvqlwlhis rerlffshff
      661 vmsffftilv wsfllsyllf iiceaptgkl eklifeplrr rmskeaigvl phadgaehkl
      721 prvaptrrae kvaslensdd kktsngwhye kgnlttchl
//
LOCUS       XP_077501153             759 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077501153
VERSION     XP_077501153.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645027.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..759
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=85469
     Region          76..202
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          296..701
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..759
                     /gene="LOC144111936"
                     /coded_by="XM_077645027.1:225..2504"
                     /db_xref="GeneID:144111936"
ORIGIN      
        1 msvqkvvliv vtvllcgclp cclggsgsdg dssasmteqq nvsyvsklke wiasamnras
       61 psltrkllea evsadcsvgl ltlvrgvrnl epwalrlida takypsgala ttrvdlgafd
      121 ecvqtvvrne yghetaraqy cnvvayggnk tdlddliaaa msithprvak frghiyeqri
      181 pimrlgicvl dvcnqdelql makavlpkvl dvtitdcvta lapdmtrtqv iilaflgtlv
      241 llivlgtavd vycsrrkqkg enkgvllsvl tsfsvvsntr mmlqittnks sdaysmrflh
      301 girffsivyi lvghsygaps diwanmvnai vyadnwanif ttvgymsvdc ffflngflla
      361 fvvhrqkrng vivfviavvr rfirtmvpvf fclmclfllp litsgpdaka yfrklndefq
      421 rqwlylllqv qnydfevtvd tpifghlwyl sldfqfflvs lpillllksr priaaaafvl
      481 lsfvgcsvat wqvagnemtp fivpltesit tflrtgfhyy fypfyhavcy fvgcmtyffv
      541 gifkekkisk acqfaawlfa iacglfcifi kvpwyqvkdp ttefgklsla ffdrilwsil
      601 mswvtiacat grggflntfl swsfftplsr lafgvyiihv pfvqlwlhis rerlfsshff
      661 vmsffftilv wsfllsyllf iiceaptgkl eklifeplrr rmskeaigvl phadgaehkl
      721 prvaptrrae kvaslensdd kktsngwhye kgnlttchl
//
LOCUS       XP_077501154             149 aa            linear   INV 25-NOV-2025
DEFINITION  N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D-like
            [Amblyomma americanum].
ACCESSION   XP_077501154
VERSION     XP_077501154.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645028.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..149
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..149
                     /product="N-acyl-phosphatidylethanolamine-hydrolyzing
                     phospholipase D-like"
                     /calculated_mol_wt=17038
     Region          <16..120
                     /region_name="UlaG"
                     /note="L-ascorbate lactonase UlaG, metallo-beta-lactamase
                     superfamily [Carbohydrate transport and metabolism];
                     COG2220"
                     /db_xref="CDD:441822"
     Region          <16..66
                     /region_name="metallo-hydrolase-like_MBL-fold"
                     /note="mainly hydrolytic enzymes and related proteins
                     which carry out various biological functions; MBL-fold
                     metallohydrolase domain; cl23716"
                     /db_xref="CDD:451500"
     CDS             1..149
                     /gene="LOC144111937"
                     /coded_by="XM_077645028.1:154..603"
                     /db_xref="GeneID:144111937"
ORIGIN      
        1 mltdylgvil fllqvlwggw cvispeyrfy fagdtgycdv fkqigqaygp fdvaaipiga
       61 yeprwfmkyq hvnpeeavli hkdvrsratl avhwgtftla neyyldppvk lresldrhgi
      121 sprefftlkh gesrllhatt snrtsrvlp
//
LOCUS       XP_077501155             558 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111938 [Amblyomma americanum].
ACCESSION   XP_077501155
VERSION     XP_077501155.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645029.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..558
                     /product="uncharacterized protein LOC144111938"
                     /calculated_mol_wt=62165
     Region          <22..>163
                     /region_name="PHA03379"
                     /note="EBNA-3A; Provisional"
                     /db_xref="CDD:223066"
     CDS             1..558
                     /gene="LOC144111938"
                     /coded_by="XM_077645029.1:42..1718"
                     /db_xref="GeneID:144111938"
ORIGIN      
        1 mseggpfngl eeeeevcrkr srpeivslts pltpphsvpe rlsreaspip aprlskhsrl
       61 prpvevqets pipaprlskd srlprpvevq easptpasql tdnsclprpv evqeasptla
      121 pqltensclp rpvevqeasp tpasqlndns clprpvevqe asptlvprli eksclprpve
      181 vqegkreeaf aymqflqspa haqlgelals eislskevra fldqlrtlas lseqsdsvav
      241 lvsntehntf lcavltllsr cktnlivkfc qgkckaplft kmenllliam ahaapdrqaa
      301 llfvliarfc siwqpksllp qaayvrfmfa lcrslgerav ardmiwelmr sdrasflvas
      361 avgawpglfd alspgpvrnv isyvlhrcps nelsdtllsy cktvldklgp lpcfdgnlyp
      421 lvddllhpll tthecmdtcl elrkaleeic rrspnsilpw vlrkqllpll qsllsklqlp
      481 laqqhatcli rllgplwees kkfpkrldpl leqmadclah cgsplrgaii eafkrlpgfq
      541 ksrwpaelsy wflhwnrs
//
LOCUS       XP_077501156             558 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111938 [Amblyomma americanum].
ACCESSION   XP_077501156
VERSION     XP_077501156.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645030.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..558
                     /product="uncharacterized protein LOC144111938"
                     /calculated_mol_wt=62165
     Region          <22..>163
                     /region_name="PHA03379"
                     /note="EBNA-3A; Provisional"
                     /db_xref="CDD:223066"
     CDS             1..558
                     /gene="LOC144111938"
                     /coded_by="XM_077645030.1:42..1718"
                     /db_xref="GeneID:144111938"
ORIGIN      
        1 mseggpfngl eeeeevcrkr srpeivslts pltpphsvpe rlsreaspip aprlskhsrl
       61 prpvevqets pipaprlskd srlprpvevq easptpasql tdnsclprpv evqeasptla
      121 pqltensclp rpvevqeasp tpasqlndns clprpvevqe asptlvprli eksclprpve
      181 vqegkreeaf aymqflqspa haqlgelals eislskevra fldqlrtlas lseqsdsvav
      241 lvsntehntf lcavltllsr cktnlivkfc qgkckaplft kmenllliam ahaapdrqaa
      301 llfvliarfc siwqpksllp qaayvrfmfa lcrslgerav ardmiwelmr sdrasflvas
      361 avgawpglfd alspgpvrnv isyvlhrcps nelsdtllsy cktvldklgp lpcfdgnlyp
      421 lvddllhpll tthecmdtcl elrkaleeic rrspnsilpw vlrkqllpll qsllsklqlp
      481 laqqhatcli rllgplwees kkfpkrldpl leqmadclah cgsplrgaii eafkrlpgfq
      541 ksrwpaelsy wflhwnrs
//
LOCUS       XP_077501157             365 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111940 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501157
VERSION     XP_077501157.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645031.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..365
                     /product="uncharacterized protein LOC144111940 isoform X1"
                     /calculated_mol_wt=38606
     Region          <118..>170
                     /region_name="APC10-like"
                     /note="APC10-like DOC1 domains in E3 ubiquitin ligases
                     that mediate substrate ubiquitination; cl02148"
                     /db_xref="CDD:382862"
     CDS             1..365
                     /gene="LOC144111940"
                     /coded_by="XM_077645031.1:429..1526"
                     /db_xref="GeneID:144111940"
ORIGIN      
        1 msgihrdrrq ragspkrepk psgrprpssq cmasvgfett gstlagnrvd cscvpsafis
       61 ttrrsptray irsvwrrsap rqlprrslai wwaaaashqh sfppqgdllr ahifavsgvg
      121 rlrnncldar wpssrlqlpp isiqfhckvt iqgicicadc mrgescnpnr rfgsvhrhsq
      181 pqatavrrdv apeptppvhf ccpasqspas pppspqgqen sevtcaddtt pkaagradtd
      241 hgcfevltsr ppvglqpsdi tgavtplcsl iqqhqaafva htsearklvd ecaaanvstl
      301 sllqstvqgl qeqqtaqmat glnimgltsp tagavgvsss sggvgqchhi saisisspsa
      361 dpgpg
//
LOCUS       XP_077501158             365 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111940 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501158
VERSION     XP_077501158.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645032.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..365
                     /product="uncharacterized protein LOC144111940 isoform X1"
                     /calculated_mol_wt=38606
     Region          <118..>170
                     /region_name="APC10-like"
                     /note="APC10-like DOC1 domains in E3 ubiquitin ligases
                     that mediate substrate ubiquitination; cl02148"
                     /db_xref="CDD:382862"
     CDS             1..365
                     /gene="LOC144111940"
                     /coded_by="XM_077645032.1:420..1517"
                     /db_xref="GeneID:144111940"
ORIGIN      
        1 msgihrdrrq ragspkrepk psgrprpssq cmasvgfett gstlagnrvd cscvpsafis
       61 ttrrsptray irsvwrrsap rqlprrslai wwaaaashqh sfppqgdllr ahifavsgvg
      121 rlrnncldar wpssrlqlpp isiqfhckvt iqgicicadc mrgescnpnr rfgsvhrhsq
      181 pqatavrrdv apeptppvhf ccpasqspas pppspqgqen sevtcaddtt pkaagradtd
      241 hgcfevltsr ppvglqpsdi tgavtplcsl iqqhqaafva htsearklvd ecaaanvstl
      301 sllqstvqgl qeqqtaqmat glnimgltsp tagavgvsss sggvgqchhi saisisspsa
      361 dpgpg
//
LOCUS       XP_077501159             343 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111940 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501159
VERSION     XP_077501159.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645033.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..343
                     /product="uncharacterized protein LOC144111940 isoform X2"
                     /calculated_mol_wt=37176
     Region          <118..>170
                     /region_name="APC10-like"
                     /note="APC10-like DOC1 domains in E3 ubiquitin ligases
                     that mediate substrate ubiquitination; cl02148"
                     /db_xref="CDD:382862"
     CDS             1..343
                     /gene="LOC144111940"
                     /coded_by="XM_077645033.1:427..1458"
                     /db_xref="GeneID:144111940"
ORIGIN      
        1 msgihrdrrq ragspkrepk psgrprpssq cmasvgfett gstlagnrvd cscvpsafis
       61 ttrrsptray irsvwrrsap rqlprrslai wwaaaashqh sfppqgdllr ahifavsgvg
      121 rlrnncldar wpssrlqlpp isiqfhckvt iqgicicadc mrgescnpnr rfgsvhrhsq
      181 pqatavrrdv apeptppvhf ccpasqspas pppspqgqen sevtcaddtt pkaagradtd
      241 hgcfevltsr ppvglqpsdi tgavtplcsl iqqhqaafva htsearklvd ecaaanvstl
      301 sllqstvqgl qeqqtaqmat gllqrrcgtv pphlryfhll pic
//
LOCUS       XP_077501160             337 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111940 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501160
VERSION     XP_077501160.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645034.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..337
                     /product="uncharacterized protein LOC144111940 isoform X3"
                     /calculated_mol_wt=36372
     Region          <118..>170
                     /region_name="APC10-like"
                     /note="APC10-like DOC1 domains in E3 ubiquitin ligases
                     that mediate substrate ubiquitination; cl02148"
                     /db_xref="CDD:382862"
     CDS             1..337
                     /gene="LOC144111940"
                     /coded_by="XM_077645034.1:428..1441"
                     /db_xref="GeneID:144111940"
ORIGIN      
        1 msgihrdrrq ragspkrepk psgrprpssq cmasvgfett gstlagnrvd cscvpsafis
       61 ttrrsptray irsvwrrsap rqlprrslai wwaaaashqh sfppqgdllr ahifavsgvg
      121 rlrnncldar wpssrlqlpp isiqfhckvt iqgicicadc mrgescnpnr rfgsvhrhsq
      181 pqatavrrdv apeptppvhf ccpasqspas pppspqgqen sevtcaddtt pkaagradtd
      241 hgcfevltsr ppvglqpsdi tgavtplcsl iqqhqaafva htsearklvd ecaaanvstl
      301 sllqstvqgl qeqqtaqmat glreelfadp vlhvyga
//
LOCUS       XP_077501161             307 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111940 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077501161
VERSION     XP_077501161.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645035.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..307
                     /product="uncharacterized protein LOC144111940 isoform X4"
                     /calculated_mol_wt=31984
     Region          32..>112
                     /region_name="APC10-like"
                     /note="APC10-like DOC1 domains in E3 ubiquitin ligases
                     that mediate substrate ubiquitination; cl02148"
                     /db_xref="CDD:382862"
     Site            order(72,99,106,108)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176484"
     CDS             1..307
                     /gene="LOC144111940"
                     /coded_by="XM_077645035.1:345..1268"
                     /db_xref="GeneID:144111940"
ORIGIN      
        1 mqglliwssg arrhfvqpcs adtmssgyvr hpsrsetkgr vpeagaqavw sassqqpvsg
       61 vgrlrnncld arwpssrlql ppisiqfhck vtiqgicica dcmrgescnp nrrfgsvhrh
      121 sqpqatavrr dvapeptppv hfccpasqsp aspppspqgq ensevtcadd ttpkaagrad
      181 tdhgcfevlt srppvglqps ditgavtplc sliqqhqaaf vahtsearkl vdecaaanvs
      241 tlsllqstvq glqeqqtaqm atglnimglt sptagavgvs sssggvgqch hisaisissp
      301 sadpgpg
//
LOCUS       XP_077501162             284 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111940 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077501162
VERSION     XP_077501162.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645036.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..284
                     /product="uncharacterized protein LOC144111940 isoform X5"
                     /calculated_mol_wt=29441
     Region          9..>89
                     /region_name="APC10-like"
                     /note="APC10-like DOC1 domains in E3 ubiquitin ligases
                     that mediate substrate ubiquitination; cl02148"
                     /db_xref="CDD:382862"
     Site            order(49,76,83,85)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176484"
     CDS             1..284
                     /gene="LOC144111940"
                     /coded_by="XM_077645036.1:404..1258"
                     /db_xref="GeneID:144111940"
ORIGIN      
        1 mssgyvrhps rsetkgrvpe agaqavwsas sqqpvsgvgr lrnncldarw pssrlqlppi
       61 siqfhckvti qgicicadcm rgescnpnrr fgsvhrhsqp qatavrrdva peptppvhfc
      121 cpasqspasp ppspqgqens evtcaddttp kaagradtdh gcfevltsrp pvglqpsdit
      181 gavtplcsli qqhqaafvah tsearklvde caaanvstls llqstvqglq eqqtaqmatg
      241 lnimgltspt agavgvssss ggvgqchhis aisisspsad pgpg
//
LOCUS       XP_077501163             110 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111941 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501163
VERSION     XP_077501163.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645037.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..110
                     /product="uncharacterized protein LOC144111941 isoform X1"
                     /calculated_mol_wt=11937
     CDS             1..110
                     /gene="LOC144111941"
                     /coded_by="XM_077645037.1:375..707"
                     /db_xref="GeneID:144111941"
ORIGIN      
        1 mrrqlpplkr ntdvklddwt lltpassrng ihpmwrllll sgasystgav wsgitvctfg
       61 ihpmwrllll sgapystgav wsgitvctfg ihpmwrllll sgapystgav
//
LOCUS       XP_077501164              87 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111941 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501164
VERSION     XP_077501164.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645038.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..87
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..87
                     /product="uncharacterized protein LOC144111941 isoform X2"
                     /calculated_mol_wt=9244
     CDS             1..87
                     /gene="LOC144111941"
                     /coded_by="XM_077645038.1:35..298"
                     /db_xref="GeneID:144111941"
ORIGIN      
        1 mihtktgihp mwrllllsga systgavwsg itvctfgihp mwrllllsga pystgavwsg
       61 itvctfgihp mwrllllsga pystgav
//
LOCUS       XP_077501165             330 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111942 [Amblyomma americanum].
ACCESSION   XP_077501165
VERSION     XP_077501165.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645039.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..330
                     /product="uncharacterized protein LOC144111942"
                     /calculated_mol_wt=35964
     Region          223..>330
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     CDS             1..330
                     /gene="LOC144111942"
                     /coded_by="XM_077645039.1:402..1394"
                     /db_xref="GeneID:144111942"
ORIGIN      
        1 mregeaseif qggsarrrrc cycgdcgsqp nafevlqgvv hreggwnhrk calhlhgrpw
       61 vmlhthvaal lfrveataqy glndpsptdv vckwieaskg staapvseie ffkpkigrap
      121 pqvseapdys vpvltdeqvs rflhqvkeia pntliltsis dsedtdsape taalepvrvg
      181 rckqlsakpp laeiyqdldi degkkvelta accseieeat rgqarsprwl qerrgritas
      241 vmhrvaacst gaaglvaeim gyvktpqvsn lrwgsetekk avqafietes qkhvdfslrd
      301 cglfiarsmp figaspdalv scsccpksvl
//
LOCUS       XP_077501166            1299 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111944 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501166
VERSION     XP_077501166.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645040.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1299
                     /product="uncharacterized protein LOC144111944 isoform X1"
                     /calculated_mol_wt=141818
     Region          <107..307
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          <274..533
                     /region_name="rne"
                     /note="ribonuclease E; Reviewed; PRK10811"
                     /db_xref="CDD:236766"
     Region          <713..>839
                     /region_name="PTZ00449"
                     /note="104 kDa microneme/rhoptry antigen; Provisional"
                     /db_xref="CDD:185628"
     CDS             1..1299
                     /gene="LOC144111944"
                     /coded_by="XM_077645040.1:96..3995"
                     /db_xref="GeneID:144111944"
ORIGIN      
        1 mtatqghkhp tahgrpwlkr rirhlpsehs patisrldqa tgtetpegsm tvtqghkhpt
       61 ahgdgasder smllnmiaem gdprsltplp pspphggees kvdqfevtlp elpskspwla
      121 wsplsvasps epetsppepv pestpppess qptrlleacv qvsreeqlep atleepvvpl
      181 asfelpqdaa egqehgkeqt afapisipep glvlevtsep pqpmrlseah mqesldeqqd
      241 femrdvkelv alsvssklph etagmlepvn dtmalasvsa pdlrpvpelt ptseppqptq
      301 pswdsvqeyl eeqlepftss kreqqnlame aarelmvtsg sfelpqetam qlehaeeama
      361 pvsisalkpg llpevsplse aseprhvlva stqaslseel ksaavskmel qapvmeavee
      421 ltmtsasskl pqdiagmleh vaepralgai svqetgldvl vrseppqtaw ppdlfmlsqv
      481 aesaarcelk qenlgqeapv talpaspela papvlesval aassepvqpt ttallpqlae
      541 sglsphpeaa fesephpaaa aspaeqrdlg aelpvvcpmt vrtsaaatlt asgtgqlreg
      601 tqpaeaahlp srarpsrees sseeaeqsat tgqecplqpq qmgsvlaalm aiveklgqis
      661 dnmqlfrnel glwfrpdlhh ptelrdrrca scgqeqssgv apllvrgqev pgqeqhshde
      721 mhngrtleva pptkrrhsvh qmseggpfng leeeeevcrk rsrpeivslt spltpphsvp
      781 erlsreaspi paprlskhsr lprpvevqet spipaprlsk dsrlprpvev qeasptpasq
      841 ltdnsclprp vevqeasptl asqlndnscl prpvevqeas ptpasqlndn sclprpvevq
      901 easptlvprl ieksclprpv evqegkreea faymqflqsp ahaqlgelal seislskevr
      961 afldqlrtla slseqsdsva vlvsntehnt flcavltlls rcktnlivkf cqgkckaplf
     1021 tkmenlllia mahaapdrqa allfvliarf csiwqpksll pqaayvrfmf alcrslgera
     1081 vardmiwelm rsdrasflva savgawpglf dalspgpvrn visyvlhrcp snelsdtlls
     1141 ycktvldklg plpcfdgnly plvddllhpl ltthecmdtc lelrkaleei crrspnsilp
     1201 wvlrkqllpl lqsllsklql plaqqhatcl irllgplwee skkfpkrldp lleqmadcla
     1261 hcgsplrgai ieafkrlpgf qksrwpaels ywflhwnrs
//
LOCUS       XP_077501167            1299 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111944 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501167
VERSION     XP_077501167.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645041.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1299
                     /product="uncharacterized protein LOC144111944 isoform X1"
                     /calculated_mol_wt=141818
     Region          <107..307
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          <274..533
                     /region_name="rne"
                     /note="ribonuclease E; Reviewed; PRK10811"
                     /db_xref="CDD:236766"
     Region          <713..>839
                     /region_name="PTZ00449"
                     /note="104 kDa microneme/rhoptry antigen; Provisional"
                     /db_xref="CDD:185628"
     CDS             1..1299
                     /gene="LOC144111944"
                     /coded_by="XM_077645041.1:97..3996"
                     /db_xref="GeneID:144111944"
ORIGIN      
        1 mtatqghkhp tahgrpwlkr rirhlpsehs patisrldqa tgtetpegsm tvtqghkhpt
       61 ahgdgasder smllnmiaem gdprsltplp pspphggees kvdqfevtlp elpskspwla
      121 wsplsvasps epetsppepv pestpppess qptrlleacv qvsreeqlep atleepvvpl
      181 asfelpqdaa egqehgkeqt afapisipep glvlevtsep pqpmrlseah mqesldeqqd
      241 femrdvkelv alsvssklph etagmlepvn dtmalasvsa pdlrpvpelt ptseppqptq
      301 pswdsvqeyl eeqlepftss kreqqnlame aarelmvtsg sfelpqetam qlehaeeama
      361 pvsisalkpg llpevsplse aseprhvlva stqaslseel ksaavskmel qapvmeavee
      421 ltmtsasskl pqdiagmleh vaepralgai svqetgldvl vrseppqtaw ppdlfmlsqv
      481 aesaarcelk qenlgqeapv talpaspela papvlesval aassepvqpt ttallpqlae
      541 sglsphpeaa fesephpaaa aspaeqrdlg aelpvvcpmt vrtsaaatlt asgtgqlreg
      601 tqpaeaahlp srarpsrees sseeaeqsat tgqecplqpq qmgsvlaalm aiveklgqis
      661 dnmqlfrnel glwfrpdlhh ptelrdrrca scgqeqssgv apllvrgqev pgqeqhshde
      721 mhngrtleva pptkrrhsvh qmseggpfng leeeeevcrk rsrpeivslt spltpphsvp
      781 erlsreaspi paprlskhsr lprpvevqet spipaprlsk dsrlprpvev qeasptpasq
      841 ltdnsclprp vevqeasptl asqlndnscl prpvevqeas ptpasqlndn sclprpvevq
      901 easptlvprl ieksclprpv evqegkreea faymqflqsp ahaqlgelal seislskevr
      961 afldqlrtla slseqsdsva vlvsntehnt flcavltlls rcktnlivkf cqgkckaplf
     1021 tkmenlllia mahaapdrqa allfvliarf csiwqpksll pqaayvrfmf alcrslgera
     1081 vardmiwelm rsdrasflva savgawpglf dalspgpvrn visyvlhrcp snelsdtlls
     1141 ycktvldklg plpcfdgnly plvddllhpl ltthecmdtc lelrkaleei crrspnsilp
     1201 wvlrkqllpl lqsllsklql plaqqhatcl irllgplwee skkfpkrldp lleqmadcla
     1261 hcgsplrgai ieafkrlpgf qksrwpaels ywflhwnrs
//
LOCUS       XP_077501168            1250 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111944 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501168
VERSION     XP_077501168.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645042.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1250
                     /product="uncharacterized protein LOC144111944 isoform X2"
                     /calculated_mol_wt=136371
     Region          <58..258
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          <225..484
                     /region_name="rne"
                     /note="ribonuclease E; Reviewed; PRK10811"
                     /db_xref="CDD:236766"
     Region          <664..>790
                     /region_name="PTZ00449"
                     /note="104 kDa microneme/rhoptry antigen; Provisional"
                     /db_xref="CDD:185628"
     CDS             1..1250
                     /gene="LOC144111944"
                     /coded_by="XM_077645042.1:79..3831"
                     /db_xref="GeneID:144111944"
ORIGIN      
        1 mtatqghkhp tahgdgasde rsmllnmiae mgdprsltpl ppspphggee skvdqfevtl
       61 pelpskspwl awsplsvasp sepetsppep vpestpppes sqptrlleac vqvsreeqle
      121 patleepvvp lasfelpqda aegqehgkeq tafapisipe pglvlevtse ppqpmrlsea
      181 hmqesldeqq dfemrdvkel valsvssklp hetagmlepv ndtmalasvs apdlrpvpel
      241 tptseppqpt qpswdsvqey leeqlepfts skreqqnlam eaarelmvts gsfelpqeta
      301 mqlehaeeam apvsisalkp gllpevspls easeprhvlv astqaslsee lksaavskme
      361 lqapvmeave eltmtsassk lpqdiagmle hvaepralga isvqetgldv lvrseppqta
      421 wppdlfmlsq vaesaarcel kqenlgqeap vtalpaspel apapvlesva laassepvqp
      481 tttallpqla esglsphpea afesephpaa aaspaeqrdl gaelpvvcpm tvrtsaaatl
      541 tasgtgqlre gtqpaeaahl psrarpsree ssseeaeqsa ttgqecplqp qqmgsvlaal
      601 maiveklgqi sdnmqlfrne lglwfrpdlh hptelrdrrc ascgqeqssg vapllvrgqe
      661 vpgqeqhshd emhngrtlev apptkrrhsv hqmseggpfn gleeeeevcr krsrpeivsl
      721 tspltpphsv perlsreasp ipaprlskhs rlprpvevqe tspipaprls kdsrlprpve
      781 vqeasptpas qltdnsclpr pvevqeaspt lasqlndnsc lprpvevqea sptpasqlnd
      841 nsclprpvev qeasptlvpr lieksclprp vevqegkree afaymqflqs pahaqlgela
      901 lseislskev rafldqlrtl aslseqsdsv avlvsntehn tflcavltll srcktnlivk
      961 fcqgkckapl ftkmenllli amahaapdrq aallfvliar fcsiwqpksl lpqaayvrfm
     1021 falcrslger avardmiwel mrsdrasflv asavgawpgl fdalspgpvr nvisyvlhrc
     1081 psnelsdtll sycktvldkl gplpcfdgnl yplvddllhp lltthecmdt clelrkalee
     1141 icrrspnsil pwvlrkqllp llqsllsklq lplaqqhatc lirllgplwe eskkfpkrld
     1201 plleqmadcl ahcgsplrga iieafkrlpg fqksrwpael sywflhwnrs
//
LOCUS       XP_077501170             342 aa            linear   INV 25-NOV-2025
DEFINITION  AP-3 complex subunit mu-2-like [Amblyomma americanum].
ACCESSION   XP_077501170
VERSION     XP_077501170.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645044.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 28% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..342
                     /product="AP-3 complex subunit mu-2-like"
                     /calculated_mol_wt=38182
     Region          3..111
                     /region_name="AP3_Mu_N"
                     /note="AP-3 complex subunit mu N-terminal domain; cd14837"
                     /db_xref="CDD:341441"
     Region          135..>260
                     /region_name="AP_MHD_Cterm"
                     /note="C-terminal domain of adaptor protein (AP) complexes
                     medium mu subunits and its homologs (MHD); cl10970"
                     /db_xref="CDD:472082"
     CDS             1..342
                     /gene="LOC144111946"
                     /coded_by="XM_077645044.1:1..1029"
                     /db_xref="GeneID:144111946"
ORIGIN      
        1 mihsvffing sgdvftekhw ksvircsvcd ffdvqkkaas pvyippvisa phhyliniyc
       61 nkmffvavtv yegfyridws qlldemldsg fplatesnil kelikppnil rtlantvtgr
      121 tnlssqhpag qlsnvpwrrt gvkyanneay fdvveevdai inksglvisa eiqgyvdyci
      181 klsgmpdlsl tfvnpclfgn vsfhpcvrfr hwemssrser vlsfvppdgn frlvsyhies
      241 qsivfvlifc rhnisrrrga cgpplrqgiw ahedkvvseh imdvsdvvev sgelthelqt
      301 edepkasapn ngkgppastd kqespspdpk ppdpclpgsa tw
//
LOCUS       XP_077501171             193 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111947 [Amblyomma americanum].
ACCESSION   XP_077501171
VERSION     XP_077501171.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645045.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..193
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..193
                     /product="uncharacterized protein LOC144111947"
                     /calculated_mol_wt=19650
     CDS             1..193
                     /gene="LOC144111947"
                     /coded_by="XM_077645045.1:183..764"
                     /db_xref="GeneID:144111947"
ORIGIN      
        1 maraqatfla hnaavaahta gparievqfl avqealqkda agppaaaavq lwpqvpchpt
       61 sapgmlvvag lpsllppafs tplpqalsis vgarssvais hasalvgavs saerralkhg
      121 vrspccsggg ssdedllldv eserdgfsgp sgspnasqer srlcalhrsk ldpvrpqvmp
      181 plceecvfld aal
//
LOCUS       XP_077501172             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111950 [Amblyomma americanum].
ACCESSION   XP_077501172
VERSION     XP_077501172.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645046.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144111950"
                     /calculated_mol_wt=17728
     CDS             1..158
                     /gene="LOC144111950"
                     /coded_by="XM_077645046.1:78..554"
                     /db_xref="GeneID:144111950"
ORIGIN      
        1 mscafqdlvl llqenvasvv lvfkvlsrhf deprkaqfed rrrrqlnrrl satlkrlsgv
       61 hilnpehrfl dasgkpmlsl faadryhvar drgidqmski ivaalvkiyg pgiasasrar
      121 pgevyvvhrc rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077501174            3146 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111953 [Amblyomma americanum].
ACCESSION   XP_077501174
VERSION     XP_077501174.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645048.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 24% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..3146
                     /product="uncharacterized protein LOC144111953"
                     /calculated_mol_wt=339163
     CDS             1..3146
                     /gene="LOC144111953"
                     /coded_by="XM_077645048.1:1..9441"
                     /db_xref="GeneID:144111953"
ORIGIN      
        1 mvffdwvdna padalsrvta slasaappln ldfaalakvq qhdtevmdlr rnpqcaplek
       61 eiikyegtak rllmdgvqqe qqvkatsdkq rlavqqftkv kmelkemkqq weqvegevef
      121 cknaiprfia llreeqaqta tlhrsyeeeq qlpddvleve rkaqasynsr krfwlkqdka
      181 encsraliyv rtwnqaasea prdqggqrlk rrcarlpskh spakilrldq atgtetsegs
      241 mtapqghkhp tahgdgasde rsmlinmrae mgdrrsltpl ppspphggee skvdqfevtl
      301 pdlpsislwl pwslqsvasp pepetsspep vpestpppgp sqptrlleac vqvsreeqle
      361 patleepvvp lasfelpqda aegqehgkeq mafasisvpe pglvlevtse ppqpkrlsea
      421 hmqesldeqq dlamrdvkel valsvssklp hetagmlehv ndtmalasvs apdlrpvpel
      481 tptseppqpt qpsgasvqey leeqlepfts skleqqnlam eaarelmvts gsfelpqeta
      541 mqlehaeeam apvaisalnp gllpevspls easesrhvlv astqaslsee lksaavskme
      601 lqapvmeave eltmtsassk lpqdiagmle hvagpmalga isvqetgldv parseppqta
      661 wppdlfmlsq vaesaarcel kqqnlgqegp vtalpaspel apasvlesva laassepvqp
      721 tttallpqla esglsphpea vfeserrpwv krrirrplse hspatiswpd qttgtetpeg
      781 smtatqghkh ptahggqrlk rrcarlpskh spakilrldq atgtetsegs mtapqghkhp
      841 tahgdgasde rsmlinmkae mgdrrsltpl ppspphggee skvdqfevtl pdlpsislwl
      901 pwslqsvasp pepetsspep vpestpppgp sqptrlleac vqvsreeqle patleepvvp
      961 lasfelpqda aegqehgkeq mafasisvpe pglvlevtse ppqpkrlsea hmqesldeqq
     1021 dlamrdvkel valsvssklp hetagmlehv ndtmalasvs apdlrpvpel tptseppqpt
     1081 qpsgasvqey leeqlepfts skleqqnlam eaarelmvts gsfelpqeta mqlehaeeam
     1141 apvaisalnp gllpevspls easesrhvlv astqaslsee lksaavskme lqapvmeave
     1201 eltmtsassk lpqdiagmle hvagpmalga isvqetgldv parseppqta wppdlfmlsq
     1261 vaesaarcel kqqnlgqegp vtalpaspel apasvlesva laassepvqp tttallpqla
     1321 esglsphpea vfeserrpwv krrirrplse hspatiswpd qttgtetpeg smtatqghkh
     1381 ptahgrpwlk rrirhlpseh spatisrldq atgtetpegs mtvtqghkhp tahgdgasde
     1441 rsmlvnmiae mgdprsltpl ppspphggee skvdqfevtl pklpskspwl awspmsvasp
     1501 pepetsppep vpestpppep sqptrlleac vrvsreeqle patleepvvp lasfelpqda
     1561 aegqehgkeq tafapistpe pglvlevtse ppqpmrlsea hmqesldeqq dlamrdvkel
     1621 vvlsvssklp hetagtlepv ndtmalasva apdlrpvpev tptseppqpa qpswdsvqey
     1681 leeqlepfts skleqqnlam eaarelmvts gsfelpqeta mqlehaeeam apvaisalkp
     1741 gllpevspls easesrhvlv astqaslsqe lkcaavskme lqapvmeave eltmtsassk
     1801 lpqdiagmle hvagpmalga isvqetgldv parseppqta wppdlfmlsq vaesaarcel
     1861 kqqnlgqegp vtallaspel apapvhesvt laassepvqp tttallpqla efglsphpea
     1921 vfeserrpwl krrircplse hspatisrld qatgtekpeg smtatqghkh ptahgrpwlk
     1981 rrirhlpseh spatisrldq atgtetpegs mtvtqghkhp tahgdgasde rsmllnmiae
     2041 mgdprsltpl ppspphggee skvdqfevtl pelpskspwl awsplsvasp sepetsppep
     2101 vpestpppes sqptrlleac vqvsreeqle patleepvvp lasfelpqda aegqehgkeq
     2161 tafapisipe pglvlevtse ppqpmrlsea hmqesldeqq dfemrdvkel valsvssklp
     2221 hetagmlepv ndtmalasvs apdlrpvpel tptseppqpt qpswdsvqey leeqlepfts
     2281 skreqqnlam eaarelmvts gsfelpheta mqlehaeeam apvsisalkp gllpevspls
     2341 easeprhvlv astqaslsee lksaavskme lqapvmkave eltmtsassk lpqdiagmle
     2401 hvaepmalga isvqetgldv lvrseppqta wppdlfmlsq vaesaarcel kqenlgqeap
     2461 vtalpaspel apapvlesva laassepvqp tttallpqla esglsphpea afesephpaa
     2521 aaspaeqrdl gaelpvvcpm tvrtsaaatl tasgtgqlre gtqpaeaahl psrarpsree
     2581 ssseeaeqsa ttgqecplqp qqmgsvlaal maiveklgqi sdnmqlfrne lglwfrpdlh
     2641 hptelrdrrc ascgqeqssg vapllvrgqe vpgqeqncsr aliyvrtwnq aaseaprdqg
     2701 gqrlkrrcar lpskhspaki lrldqatgte tsegsmtapq ghkhptahgd gasdersmli
     2761 nmraamgdrr sltplppspp hggeeskvdq fevtlpdlps islwlpwslq svasppepet
     2821 sspepvpest pppgpsqptr lleacvqvsr eeqlepatle epvvplasfe lpqdaaegqe
     2881 hgkeqmafas isvpepglvl evtseppqpk rlseahmqes ldeqqdlamr dvkelvalsv
     2941 ssklphetag mlehvndtma lasvsapdlr pvpeltptse ppqptqpsga svqeyleeql
     3001 epftsskleq qnlameaare lmvtsgsfel pqetamqleh aeeamapvai salnpgllpe
     3061 vsplseases rhvlvastqa slseelksaa vskmelqapv meaveeltmt sassklpqdi
     3121 agmrqdwtcl lgqnhhrqlg hqtflc
//
LOCUS       XP_077501175             176 aa            linear   INV 25-NOV-2025
DEFINITION  sentrin-specific protease 1-like, partial [Amblyomma americanum].
ACCESSION   XP_077501175
VERSION     XP_077501175.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645049.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: incomplete on the carboxy end.
FEATURES             Location/Qualifiers
     source          1..176
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..>176
                     /product="sentrin-specific protease 1-like"
     Region          55..175
                     /region_name="Peptidase_C48"
                     /note="Ulp1 protease family, C-terminal catalytic domain;
                     cl23802"
                     /db_xref="CDD:420019"
     CDS             1..176
                     /gene="LOC144111954"
                     /coded_by="XM_077645049.1:1..>527"
                     /db_xref="GeneID:144111954"
ORIGIN      
        1 metssivahl egdvkhhsrl tfamqeevse alkpgppqqv lveafsiavq krggprvyaf
       61 sthfftklyr cgyegvkrwt rgvdlfayei llvpvnfada shwclavvdf rthemviyds
      121 lgprgghaes idalahylqe eshlrgrdld wggwrfyprt vplqknasdc gvficr
//
LOCUS       XP_077501176             621 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111955 [Amblyomma americanum].
ACCESSION   XP_077501176
VERSION     XP_077501176.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645050.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..621
                     /product="uncharacterized protein LOC144111955"
                     /calculated_mol_wt=70338
     Region          72..198
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          <306..562
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..621
                     /gene="LOC144111955"
                     /coded_by="XM_077645050.1:1..1866"
                     /db_xref="GeneID:144111955"
ORIGIN      
        1 mppyvvfkrq tlpkkrfsng iivrvqekgw mseeqqnvsy vsklkewias amdraapslt
       61 rklleadvsa ecsiglltlv rgirnlepla lrlidatgky psglltsara nlgafdkcve
      121 tvghnefgqk targqycnvm ayggnktdld dliaaamsit htrvtrfrgh iyeqtilimr
      181 lgicvldvcs qeelhdvvfs vlpetldati kncitglspr ittaqvtila flgtlglviv
      241 lgnavdltma plffvlmcif lmplitsgpd dkgyfhkfkd efqrqwlyll lqvknydfkv
      301 tadaplfghl wylsmdfqff lvslpillll kslrlavaaf vvlslvgcsv atwqvagnem
      361 tpfifpltes itsflkthfy yyfypfyhav cyftgcitff avasfkrskm pkvlqatawc
      421 iavacrmcci fmktvwyrtr dpttefgkls aaffdrilws iflawitlsc atgrggflnk
      481 flswsaftpl srlafgvyii hlpfihlclh isreriffsh fsvvsfffsm lvwsyllsyl
      541 lfifceaptg rldklifdpq rrktegrekd eapndnsvqd klpdavvtfr drkldiwqts
      601 sekntsngil pgncsrtsch l
//
LOCUS       XP_077501177             140 aa            linear   INV 25-NOV-2025
DEFINITION  N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D-like
            [Amblyomma americanum].
ACCESSION   XP_077501177
VERSION     XP_077501177.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645051.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..140
                     /product="N-acyl-phosphatidylethanolamine-hydrolyzing
                     phospholipase D-like"
                     /calculated_mol_wt=15976
     Region          <3..57
                     /region_name="metallo-hydrolase-like_MBL-fold"
                     /note="mainly hydrolytic enzymes and related proteins
                     which carry out various biological functions; MBL-fold
                     metallohydrolase domain; cl23716"
                     /db_xref="CDD:451500"
     Region          <7..111
                     /region_name="UlaG"
                     /note="L-ascorbate lactonase UlaG, metallo-beta-lactamase
                     superfamily [Carbohydrate transport and metabolism];
                     COG2220"
                     /db_xref="CDD:441822"
     CDS             1..140
                     /gene="LOC144111958"
                     /coded_by="XM_077645051.1:1..423"
                     /db_xref="GeneID:144111958"
ORIGIN      
        1 matkkvlwgg wcvispeyrf yfagdtgycd vfkqigqayg pfdvaaipig ayeprwfmky
       61 qhvnpeeavl ihkdvrsrat lavhwgtftl aneyyldppv klresldrhg isprefftlk
      121 hgesrllhat tsnrtsrvlp
//
LOCUS       XP_077501179             867 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111959 [Amblyomma americanum].
ACCESSION   XP_077501179
VERSION     XP_077501179.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645053.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 18% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..867
                     /product="uncharacterized protein LOC144111959"
                     /calculated_mol_wt=94504
     CDS             1..867
                     /gene="LOC144111959"
                     /coded_by="XM_077645053.1:1..2604"
                     /db_xref="GeneID:144111959"
ORIGIN      
        1 mteqleassr qelttdirhv qgldnapada lsrvtaslas aapplnldfa alakvqqhdt
       61 evmdlrrnpq caplekeiik yegtakrllm dgvqqeqqvk atsdkqrlav qqftkvkmel
      121 kemkqqweqv egevefckna iprfiallre eqaqtatlhr syeeeqqlpd dvleverkaq
      181 asynsrkrfw lkqdkaencs raliyvrtwn qaaseaprdq ggqrlkrrca rlpskhspak
      241 ilrldqatgt etsegsmtap qghkhptahg dgasdersml inmraemgdr rsltplppsp
      301 phggeeskvd qfevtlpdlp sislwlpwsl qsvasppepe tsspepvpes tpppgpsqpt
      361 rlleacvqvs reeqlepatl eepvvplasf elpqdaaegq ehgkeqmafa sisvpepglv
      421 levtseppqp krlseahmqe sldeqqdlam rdvkelvals vssklpheta gmlehvndtm
      481 alasvsapdl rpvpeltpts eppqptqpsg asvqeyleeq lepftsskle qqnlameaar
      541 elmvtsgsfe lpqetamqle haeeamapva isalnpgllp evsplsease srhvlvastq
      601 aslseelksa avskmelqap vmeaveeltm tsassklpqd iagmlehvag pmalgaisvq
      661 etgldvpars eppqtawppd lfmlsqvaes aarcelkqqn lgqegpvaal paspelapas
      721 vlesvalaas sepvqpttta llpqlaesgl sphpeavfes errpwvkrri rrplsehspa
      781 tiswpdqttg tetpegsmta tqghkhptah grpwlkrrir hlpsehspat isrldqatgt
      841 etpegsmtvt qghkhptahg kttqclt
//
LOCUS       XP_077501180            2443 aa            linear   INV 25-NOV-2025
DEFINITION  ectopic P granules protein 5 homolog [Amblyomma americanum].
ACCESSION   XP_077501180
VERSION     XP_077501180.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645054.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2443
                     /product="ectopic P granules protein 5 homolog"
                     /calculated_mol_wt=272815
     CDS             1..2443
                     /gene="LOC144111961"
                     /coded_by="XM_077645054.1:255..7586"
                     /db_xref="GeneID:144111961"
ORIGIN      
        1 meqvrvkkkp kkkqhsgqpq astssacess nlavtsakld aegavasqsc deksvvyatp
       61 qpsatgdncd easgaapegv dssacidgtd avlpsypaea vteqqddger cpeelnadqv
      121 tetvtlaapk vptapaesld shyafpsssh aaatercqvg dtkapaavav rsddalsrnr
      181 sigklypels eirdnresmr pftedqilml yesawlkark sivaefvtln eerhlnhhel
      241 yrllenylks reqlastqsv vrslqedvaq qeaclwdivp dvvsgkgrca dnkrvtgehy
      301 fqralynnda asamassaqk llkllgekhv lhmhnahtqr vqidyyfqll ldaapfrdmp
      361 kntpvssgtq pqlagsvael rtcisvlyvf lrkavqddvf vsdvqswlrm lgslllrvas
      421 ladhffllnh vvrcppgihk waislvqips pmprpentdw rqatvgcqql dyalaalati
      481 llpvesraey lqplqsalea aschepwvvl dsdgeeddpp svvywrendv vallnqvpfa
      541 gifqhlllvv sedydaarts eqgvlkliav ashlvsifht glktfnhsry kqltkrisrl
      601 irhtvqyvsd hwksfrltqg andtailmrl qieydqfflr avncifysqs tgawqfmavl
      661 pyscvstvml wqllwllhnn yeekvqqthl spseiceqlq ekgqkqsfee klcsmpqsev
      721 yflltsfanm atsregdgra fthvvalevf qitylnrnlr qlfakegrdl lsslaqkepc
      781 vvsvlldaid dhmmalgvma cylmqsmplh lwvpdkqdld vishfllyyp ldspqsqlar
      841 llienlnygf neqgqlslqh clhqqlalll leaynklcvp feaagyvykq vrylsylatg
      901 ttgetstpmg faawvwttlf qlklhafdsn helqlrlvmd etpppdiapd leqcewlhpl
      961 lraskdlrpp alflsvsigt ighyreqvlg tglklvstli lneqhaaavk cighilplfy
     1021 lhqdmllgnq qfledlqrlv ladntylaaa kslvgseqqg tvlkelaamm tclvqqakrw
     1081 glpslpirlw tallfhlpdt pmhraaykpk grenvmhlld vvvqlayfea dcldsvlahf
     1141 tellatlpap tasqsmmrsv fsymlgsqsg swpamvpfqs aptfpwfsla gmlaeaqlpe
     1201 vatawkslls aiadspqvsm evlvkktmqa pldvlllyrw ayqalqtdad hpalpliwqq
     1261 ffslylqrcp ngnsagprff estsyfsllk kmkrrlsela dhsyqrcant deeepsrdll
     1321 erlfkmyrtf slwleeprlq tasidfsalp sqycpdalca iiqgncqlwh ellsleegqr
     1381 klqslqqlpq rpsasrpcrn qqqhceqape eriisrlkty egqmpapsmp mlrpiipavh
     1441 clapqvrelv saqlrvlvge astvvgrldk ltcldhicre ellpilydnv aafvhlsvpc
     1501 dyreeckgpl hlrlqyyear eapdishkie knrtewiatl telqrappha sccaqvqlea
     1561 cltqlvqrhr qatpgtkeal qklgsevffd vvgflhkemv qylptrqfvt lcldmvgeef
     1621 vanradqclp vlqailndps mvghvapffs pgaaneeqya lmyqsvssci ipslynavfv
     1681 mlskfdlpgw lishepskav rqqllvavek alckcgispq apllplvevf hlhlysllqf
     1741 hfpehyafvl amllrgtesc siavttwgvf lqalgycmpd kvvktydeey akhqclltla
     1801 eaggsidtta dhftglrksd astaksglyg rvrpflpvlp rffsviahcy iweklkapsa
     1861 nlqeavnivl rlygpwlelt dgksmclpwl pgdtkdascm adslvtvlaf fhrccqdawn
     1921 vnvlslawqh yfikyvwtnp peyittavqt afatlpwsqf cpstddmrla cklleakysg
     1981 hldflvnvft qvqwkerlde tlqlaaecvv eyhscfaelv vslawhqnms afvattvgpl
     2041 hehewgmlpv elvslvqkkl acncdvrqll kaspgtdktm ldfvatlscm spgtkrdpnk
     2101 qlsfvttlvg lygpaleaae vqdvtmllpq lldqcqmvqg svpvlgrals lldsgrkgsa
     2161 qeqallggll pwlderpghp vllavlaaac tniasvqhlv cvteaclaaf feentaadgg
     2221 waqaaaafrv peltvadfre qcamqgahlt qmcylmhclp qcrspedera lldqladwvs
     2281 qgraggesep kllllwakll tlslrqldfg gspqvvarll gnfcatlgvl gedrdtggll
     2341 galgmgrrst vsvqfrfccr vvaafvaarl tdnaavanqm lsrlqvlqtt kayqpllqei
     2401 qealnivqdt sltlgdslhm iqtlvnffyc ekaylrvlff gtv
//
LOCUS       XP_077501182             759 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077501182
VERSION     XP_077501182.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645056.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..759
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=85529
     Region          76..202
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          296..701
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..759
                     /gene="LOC144111964"
                     /coded_by="XM_077645056.1:225..2504"
                     /db_xref="GeneID:144111964"
ORIGIN      
        1 msvqkvvliv vtvllcgclp cclggsgsdg dssasmteqq nvsyvsklke wiasamnras
       61 psltrkllea evsadcsvgl ltlvrgvrnl epwalrlida takypsgala ttrvdlgafd
      121 ecvqtvvrne yghetaraqy cnvvayggnk tdlddliaaa msithprvak frghiyeqri
      181 pimrlgicvl dvcnqdelql makavlpkvl dvtitdcvta lapdmtrtqv iilaflgtlv
      241 llivlgtavd vycsrrkqkg enkgvllsvl tsfsvvsntr mmlqittnks sdaysmrflh
      301 girffsivyi lvghsygaps diwanmvnai vyadnwanif ttvgymsvdc ffflngflla
      361 fvvhrqkrng vivfviavvr rfirtmvpvf fclmclfllp litsgpdaka yfrklndefq
      421 rqwlylllqv qnydfevtvd tpifghlwyl sldfqfflvs lpillllksr priaaaafvl
      481 lsfvgcsvat wqvagnemtp fivpltesit tflrtgfhyy fypfyhavcy fvgcmtyffv
      541 gifkekkisk acqfaawlfa iacglfcifi kvpwyqvkdp ttefgklsla ffdrilwsil
      601 mswvtiacat grggflntfl swsfftplsr lafgvyiihv pfvqlwlhis rerlffshff
      661 vmsffftilv wsfllsyllf iiceaptgkl eklifeplrr rmskeaigvl phadgaehkl
      721 prvaptrrae kvaslensdd kktsngwhye kgnlttchl
//
LOCUS       XP_077501183             494 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 1-like
            [Amblyomma americanum].
ACCESSION   XP_077501183
VERSION     XP_077501183.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645057.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526491) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..494
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 1-like"
                     /calculated_mol_wt=55960
     Region          <23..82
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          23..50
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          52..83
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          113..229
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          278..393
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          392..463
                     /region_name="BACK_ABTB1_BPOZ"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     ankyrin repeat and BTB/POZ domain-containing protein 1
                     (ABTB1); cd18497"
                     /db_xref="CDD:350572"
     CDS             1..494
                     /gene="LOC144111967"
                     /coded_by="XM_077645057.1:68..1552"
                     /db_xref="GeneID:144111967"
ORIGIN      
        1 mrlekdalia eshdglrrnt lelflscrtg dlarvkylve eqeselnvrd rwdgtplyya
       61 clcghkdvve yllfqgascv antfdgercl yaslsleird llrdrkvits ntmrrdtyde
      121 flrrcledsq hcdvsfsvqg eiipahrcvl aarcdffrrs lldkwagrql ipvthhavdg
      181 avfriitqyl ytgrlemqak hfesflelas rcqlisliad vkealaevln qgsqrsgvsa
      241 esetillept hyreqlqrdf stlpvelasg vtaggaafpa eehnhadici nvdgthflch
      301 kvllcnrsey fraliedhft easrlgsdqq qlpvielqqv tpnifgcvlr hvysnsddrv
      361 natnvwdvlc aadvyllpdl krqcgaqiar lldvesicgt lrasrlfrlp rlenqciefm
      421 akhlaeviel pefneaired akevklrqet dsitviddir yyisanarst aeildanvkl
      481 kliddllttl glda
//
LOCUS       XP_077501185             306 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111971 [Amblyomma americanum].
ACCESSION   XP_077501185
VERSION     XP_077501185.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645059.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526492) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..306
                     /product="uncharacterized protein LOC144111971"
                     /calculated_mol_wt=35462
     Region          <78..>188
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          117..>165
                     /region_name="HOOK"
                     /note="HOOK protein coiled-coil region; pfam05622"
                     /db_xref="CDD:461694"
     CDS             1..306
                     /gene="LOC144111971"
                     /coded_by="XM_077645059.1:1..921"
                     /db_xref="GeneID:144111971"
ORIGIN      
        1 mpldehcgsc dmqleeldsk idcsdcrsvy htgkcagisd aaikskgeqy rsawrcsncr
       61 rskgrtphld rssndtddqd vrallksine kleyllplke tvdsaedtvq ymsdqydell
      121 trveknerev relkhrvdki ekqddeitql knemdclewr srklnlefhg ikamkdenli
      181 deinklatln nlpqlqesdi vsthrlpskq dkipgiicrf akqsvrdkww qnrktlcqgn
      241 dsifvlenlt krtstlltet klwakannyk yvwhnnnkvl vrkadgqnav viscsrdrdk
      301 lkqkis
//
LOCUS       XP_077501186             306 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111972 [Amblyomma americanum].
ACCESSION   XP_077501186
VERSION     XP_077501186.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645060.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526492) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..306
                     /product="uncharacterized protein LOC144111972"
                     /calculated_mol_wt=35395
     Region          79..>168
                     /region_name="PRK05771"
                     /note="V-type ATP synthase subunit I; Validated"
                     /db_xref="CDD:235600"
     CDS             1..306
                     /gene="LOC144111972"
                     /coded_by="XM_077645060.1:1..921"
                     /db_xref="GeneID:144111972"
ORIGIN      
        1 mpldehcgsc dmqleeldsk idcsdcrsvy htgkcagisd atikskgeqy rsawrcsncr
       61 rskgrtppld rssndtddqd vrallksine kleyllplke tvgsvedtvq ymsdqydell
      121 trveknerev relkhrvdki ekqddeitql knemdclewr srklnlefhg ikamkdenli
      181 deinklatln nlpqlqesdi vsthrlpskq dkipgiicrf akqsvrdkww qnrktlcqgs
      241 dsifvlenlt krtrtlltet klwakannyk yvwhnnnkvl vrkadgqnav viscssdrdk
      301 lkqqis
//
LOCUS       XP_077501187             234 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111974 [Amblyomma americanum].
ACCESSION   XP_077501187
VERSION     XP_077501187.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645061.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526492) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..234
                     /product="uncharacterized protein LOC144111974"
                     /calculated_mol_wt=26457
     Region          23..>120
                     /region_name="PRK05431"
                     /note="seryl-tRNA synthetase; Provisional"
                     /db_xref="CDD:235461"
     Region          81..230
                     /region_name="Baculo_FP"
                     /note="Baculovirus FP protein; pfam03258"
                     /db_xref="CDD:367421"
     CDS             1..234
                     /gene="LOC144111974"
                     /coded_by="XM_077645061.1:1..705"
                     /db_xref="GeneID:144111974"
ORIGIN      
        1 mgdkppgtik dllkyvndqn avlrkeveel kqsmtfmnds feafrttgee lealkkehaa
       61 lklekdelaq tlaktqkeli elkqysrlsn veikgipete deslvevvqk igekigmdve
      121 ttdidvvhrv ptkektkknv ivkfasraar dkflqsarkk rlsandigfs ettpvylneh
      181 lcpeykallg maiarkkann wkfvwvsnsk ilarkaenst vihiatrddl skiv
//
LOCUS       XP_077501189             693 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111975 [Amblyomma americanum].
ACCESSION   XP_077501189
VERSION     XP_077501189.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645063.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526493) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..693
                     /product="uncharacterized protein LOC144111975"
                     /calculated_mol_wt=77442
     Region          235..378
                     /region_name="Transposase_21"
                     /note="Transposase family tnp2; cl29371"
                     /db_xref="CDD:475188"
     CDS             1..693
                     /gene="LOC144111975"
                     /coded_by="XM_077645063.1:658..2739"
                     /db_xref="GeneID:144111975"
ORIGIN      
        1 metevmskwr ryriiekeyr ellgelgirr ppsthlkssg skrtggeaal dvvsapplpl
       61 psgaskrpns eacasgacas gsgratsslp adpsmpsvqs elneqayvse scaeslsseh
      121 tatscdssde tsstnsltae nvemgqpree spelcyrhls taqelasias khnlthacin
      181 dlldfcrrrg ivelpkdart imqterkanl ehgdsfvhfg laegiqhavg qgpapenvel
      241 hanidglplh rssqtalwpi lcrvinikks epfmvsaycg agkppclqey lrpfidevhn
      301 lscnglmvkg vhvnvclkav icdaparsfv kaitghtgyh acercsqkgh ylenrmtfpd
      361 lhapqrtdss frsqedrrhh tgvspftsln vdmvkcfpte ymhlvclgvm rrllrnwick
      421 ghaarlsrad rsalndklre aatafpkyfq rkprgteeld rwkatefrtf llylgpvllk
      481 silpdelykh flylhvsvrv laspqhhrdl nqfahdllry fvqefgrlyg qkqlvynvht
      541 lahlaeqcle hgtvdefsaf pfesylgkvk krlrttnkpl aqlsrrmsev ractplstgq
      601 vggdvkvgdc flvdncavvi vdllggnaka gilpngreff rvpidssrmd vrrysalgqe
      661 tkiwpisyik nkvrciklqy krghvvfpll hlq
//
LOCUS       XP_077501190             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111976 [Amblyomma americanum].
ACCESSION   XP_077501190
VERSION     XP_077501190.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645064.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526493) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144111976"
                     /calculated_mol_wt=23436
     CDS             1..217
                     /gene="LOC144111976"
                     /coded_by="XM_077645064.1:1347..2000"
                     /db_xref="GeneID:144111976"
ORIGIN      
        1 mppkpfavvk fpmeddslav vpvgwlshdw qhchwpkrra aevirlaree adpsapgcew
       61 frcpvavvtt crsyknaekk akkyemssav essaisgneg pdgpdvrlps ppprlpsppp
      121 rlpsppprlp spppqlpspp rlisppslls shsmeaatpa ganctagcpa gsastagdle
      181 dveeatsdlk aflflylymk slkrrecksh nllktdq
//
LOCUS       XP_077501191             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111977 [Amblyomma americanum].
ACCESSION   XP_077501191
VERSION     XP_077501191.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645065.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526493) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 16% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144111977"
                     /calculated_mol_wt=17966
     CDS             1..158
                     /gene="LOC144111977"
                     /coded_by="XM_077645065.1:224..700"
                     /db_xref="GeneID:144111977"
ORIGIN      
        1 mtdaeqhraq paapvtsllp ppkaldttgd vwhsweawkq efelfstaty ldqqpkespv
       61 qrlmgrqtrt llpvptshlq pqtvppktvr rrledirskq rcfynrgtrp lpelrtgsta
      121 tmynvpsrsw spvtvvqrae tprayivkte egqhipld
//
LOCUS       XP_077501192             114 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111978 [Amblyomma americanum].
ACCESSION   XP_077501192
VERSION     XP_077501192.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645066.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526493) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 18% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..114
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..114
                     /product="uncharacterized protein LOC144111978"
                     /calculated_mol_wt=13129
     CDS             1..114
                     /gene="LOC144111978"
                     /coded_by="XM_077645066.1:224..568"
                     /db_xref="GeneID:144111978"
ORIGIN      
        1 mtdaeqhraq paapvtsllp ppkaldttgd vwhsweawkq efelfstaty ldqqpkespv
       61 qrlmgrqtrt llpvptshlq pqtvppktvr rrlegirskq rcfynrvsrw iepe
//
LOCUS       XP_077501193             253 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111979 [Amblyomma americanum].
ACCESSION   XP_077501193
VERSION     XP_077501193.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645067.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526493) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..253
                     /product="uncharacterized protein LOC144111979"
                     /calculated_mol_wt=26518
     CDS             1..253
                     /gene="LOC144111979"
                     /coded_by="XM_077645067.1:21..782"
                     /db_xref="GeneID:144111979"
ORIGIN      
        1 mlkvlrllle irqqqreilq rvsrleqarq eprtrspspp vsslpplcpq lpafsiedfe
       61 aaeaavrddr vaaalkkqll rlggnnlkev aanvmgavmg vavqrlfslr grkgkrpfvg
      121 tklcrvatda icerqgvdil aaqgfigrwl pgacdrgggr krryaqalep peshaqappa
      181 npcagsapcp gapsascpat ppgmailsps gvhpssappt asssrpatlh pssapspapp
      241 pphsllvtps hpt
//
LOCUS       XP_077501194             907 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 1-like [Amblyomma americanum].
ACCESSION   XP_077501194
VERSION     XP_077501194.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645068.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526494) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..907
                     /product="endothelin-converting enzyme 1-like"
                     /calculated_mol_wt=101000
     Region          29..>247
                     /region_name="DUF612"
                     /note="Protein of unknown function, DUF612; pfam04747"
                     /db_xref="CDD:282585"
     Region          303..905
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..907
                     /gene="LOC144111980"
                     /coded_by="XM_077645068.1:38..2761"
                     /db_xref="GeneID:144111980"
ORIGIN      
        1 mdldpppmds tktskafras kkakkkvaqq skdterekds srtsrkkrki gaersperhs
       61 gtrtqevade rnqasdqagt healadtpti kaadvpgttt tdeqptlsql ptaehgdgta
      121 mrlqpqglte tegtnmphss egqallptas ddlaaapsga padlttvpae pvesadteph
      181 httrtirkvt raqasveaee tikpschedq psarsqsaqa qptvertqdg kkppgfrmms
      241 pdvpkrstci kccagtscvl lavivavhla lrapsrerhy etcdtlacaa yskrlletin
      301 ssvkpcdsft hyvcdgwnsr yeasvhdval lsaltrmyyi vrdmnvpksg qnimeraaaf
      361 ylscecvgka rcnellnvav vleaanvvwp lkaqrpdllh tllhvsirlg wagilafevk
      421 atgpkkyvll rppqefyilg qrakeqlrsp gsrqhyfevl ktslkaassa avhgdsvtfe
      481 etrnielkiw ddlwkraalh ptsimkeglf ynfvpnltle rwkqalrdyr vglredervv
      541 fmsdsipfvh affnmwkkyg eeemhlllsw ctvqvaawfa nqellinfyg teqkveivhr
      601 afcfsktfvv tgdallhrfs datlparirp qaralildvr qslhgrllrw kfydenltvv
      661 hnwnstsval lyfkkpgggd sddkseatdg hqeglvdmge slvanwqlaa qgsflnpssw
      721 rlskviqelg lyvlhadtee pdasllpyaf sfpyfdhgat dalnyagigs rvaealsqlf
      781 fsayftspea ldafknqfhc menstsnkiy rrsyapylep lslktalyay rarnaatdvr
      841 vrglefltpe qlffvaacyv rcvrpsgppg tvdgqcdktf qhvaefadaf qcapgtrmnp
      901 shrcell
//
LOCUS       XP_077501195             226 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111981 [Amblyomma americanum].
ACCESSION   XP_077501195
VERSION     XP_077501195.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645069.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526494) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..226
                     /product="uncharacterized protein LOC144111981"
                     /calculated_mol_wt=24135
     CDS             1..226
                     /gene="LOC144111981"
                     /coded_by="XM_077645069.1:48..728"
                     /db_xref="GeneID:144111981"
ORIGIN      
        1 mdstktsres rtskkakkkg aqhskdtere kdssrtsrkk rkcgterspe rhsgartqel
       61 adeskqasgq asthealvda ptikaadvpg ttgsdqqptl shlptaergd vttsrlqpqc
      121 laetkgtnmp pssegqallp kasddlaaap sgappdlttv paeqvqsadt eprhttrtir
      181 katrvqpgve aeettrpscp eeqpnarsqs aqaqptvert edgkkh
//
LOCUS       XP_077501196             210 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 1-like [Amblyomma americanum].
ACCESSION   XP_077501196
VERSION     XP_077501196.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645070.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526494) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..210
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..210
                     /product="endothelin-converting enzyme 1-like"
                     /calculated_mol_wt=23293
     Region          <56..208
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..210
                     /gene="LOC144111983"
                     /coded_by="XM_077645070.1:33..665"
                     /db_xref="GeneID:144111983"
ORIGIN      
        1 mgeslvanwq laargsflnl ysrrlskviq elglyilhad tkepdvsllp yafsfpyfdh
       61 gatdalnyag igsrmaeals qlffsayfts pkasaafknq fhcmvnatss kidrtsyapy
      121 leplsletal yayrarntgt dvrvrglefl tpeqlffvaa cyvrcvrpsg prgvkdgqcd
      181 kafrhvaefa dafqcapgtr mnpshrcell
//
LOCUS       XP_077501197             472 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111984 [Amblyomma americanum].
ACCESSION   XP_077501197
VERSION     XP_077501197.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645071.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526494) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 18% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..472
                     /product="uncharacterized protein LOC144111984"
                     /calculated_mol_wt=52133
     Region          <211..272
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     Region          325..404
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(338,340,342,363..365,402)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133137"
     Site            338..340
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133137"
     Site            338
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133137"
     Site            order(363..366,370,371..372)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133137"
     CDS             1..472
                     /gene="LOC144111984"
                     /coded_by="XM_077645071.1:1..1419"
                     /db_xref="GeneID:144111984"
ORIGIN      
        1 mklelqraan hsaevhstre egsglridas rllprfdekr ndldaflrrf egiaesqnws
       61 dgecatvlst clsgealsvf grlspddaas yskvkaallk rfrltterfr dkfrtgkptd
      121 getttqyaar lshyfdrwie lseiaqeyst lrellikeqf lsschsslsl ylkerraktl
      181 semldladqf leaqggtnlt kikntnsesa gktdsrdarf spkappkcyl cnklghyasn
      241 crnsatnssp tvcfkcgqrg hraavcpnps kpipqvscvr assghtleed engfvelkns
      301 kkvpvvnaiv nalpeaemnr mplrkgkvge ktismlrdtg ssaviikrrl vrgkdltgtk
      361 slvrledgsf qclpeakvha qtpyysgklt alcmerpiyd lilgnikgak fpkgpeteaq
      421 fvatvesvfa rnfcctfthf iglvatahhc hdvficggyf tcnaagvgdd dd
//
LOCUS       XP_077501198             212 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111985 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501198
VERSION     XP_077501198.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645072.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526494) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..212
                     /product="uncharacterized protein LOC144111985 isoform X1"
                     /calculated_mol_wt=23343
     Region          164..194
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(168,176,187..188)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(180,183,187,193..194)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            190..194
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     CDS             1..212
                     /gene="LOC144111985"
                     /coded_by="XM_077645072.1:119..757"
                     /db_xref="GeneID:144111985"
ORIGIN      
        1 msniqrvhrs favipfllls lficmhrafa dpnkyfntss lcmtgphyti frtidgavlt
       61 sesennrdcf ivfqtdsilk rfmlrfekla ldcndhlkvf dgsqpignhk vdlscgstht
      121 dvgtiftltn fvtleyttdn ssppengfkl iitaykdiqp lglncqdfec lnsfcissnl
      181 tcdgvnhcgd nsdetshasc igstlssavt ap
//
LOCUS       XP_077501199             202 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111985 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501199
VERSION     XP_077501199.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645073.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526494) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..202
                     /product="uncharacterized protein LOC144111985 isoform X2"
                     /calculated_mol_wt=22428
     Region          164..194
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(168,176,187..188)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(180,183,187,193..194)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            190..194
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     CDS             1..202
                     /gene="LOC144111985"
                     /coded_by="XM_077645073.1:119..727"
                     /db_xref="GeneID:144111985"
ORIGIN      
        1 msniqrvhrs favipfllls lficmhrafa dpnkyfntss lcmtgphyti frtidgavlt
       61 sesennrdcf ivfqtdsilk rfmlrfekla ldcndhlkvf dgsqpignhk vdlscgstht
      121 dvgtiftltn fvtleyttdn ssppengfkl iitaykdiqp lglncqdfec lnsfcissnl
      181 tcdgvnhcgd nsdetshasc ig
//
LOCUS       XP_077501201             900 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 1-like [Amblyomma americanum].
ACCESSION   XP_077501201
VERSION     XP_077501201.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645075.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526494) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..900
                     /product="endothelin-converting enzyme 1-like"
                     /calculated_mol_wt=99805
     Region          1..>240
                     /region_name="DUF612"
                     /note="Protein of unknown function, DUF612; pfam04747"
                     /db_xref="CDD:282585"
     Region          296..898
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..900
                     /gene="LOC144111988"
                     /coded_by="XM_077645075.1:1..2703"
                     /db_xref="GeneID:144111988"
ORIGIN      
        1 mditktskes rtskkakkkg aqhsvdtere kdssrssrkk rksgaerspe rdsgartqel
       61 ddeskqasgq agthealvda ptikaadvpg ttgsdqqptl sylptakcgd vtasrlqpqg
      121 ltetdgtnmp pssegqallp tasddlaaap sgappdlttv paeqvqsadt eprhttrsir
      181 katrvkpgve aeettkpscp aeqpnarsqr aqaqptvert edgkkpsafr imspdvpkgs
      241 tcikccagts cvllavivav llalqapsre rhyetcdtla caayskrlle tinssvkpcd
      301 sfthyvcdgw nsryeasvnd vallsaltrm yhivrdmnvp ksgqnemera aafylscecv
      361 gkgrcnelln valaleaanv vwphkaqrpd llrtllhvsi qlrwagilaf eveatgpkry
      421 vllrlpqefy ilgrrakeql rspgsrqhyf evlktslkaa ssaavhgdsv tfeetrniel
      481 kiwdvlwnra tvhptfimkd glfynfvpnl tlerwklalr dygvalrdne rvvfvsdsip
      541 flhaffnmwk kygeekmhll lswctvqvav wfanqellin fygteqkvei mhrafcfskt
      601 fvvtgdallh rlsdaalpag irpqaralil dvrqslhsrl lrwkfydenv tvvhdwnsts
      661 vallyfekpg evdsddksea tdvhqeglvd mgeslvanwq laarasflnp ssrrlskviq
      721 elglyvlhad teepdvsllp yafsfpyfdh gatdalnyag igsrmaeals qlffsayfts
      781 peasaafknq fycmmngtss kmdqksygpy leplslktal yayrarntgt dvrvrglefl
      841 tpeqlffvaa cyvrcvrpsg ppgvadgqcd kafqhvaefa dafqcppgtr mnpshrcell
//
LOCUS       XP_077501202             439 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111989 [Amblyomma americanum].
ACCESSION   XP_077501202
VERSION     XP_077501202.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645076.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526494) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..439
                     /product="uncharacterized protein LOC144111989"
                     /calculated_mol_wt=43866
     Region          <49..429
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..439
                     /gene="LOC144111989"
                     /coded_by="XM_077645076.1:1..1320"
                     /db_xref="GeneID:144111989"
ORIGIN      
        1 mrpldsflss sclpdglpti vleeaaavds caseedlava saeatfaipa vpvappatav
       61 prkgtsdtaa adttvtlgqg avartpagqc dsatpdssla pvgeeaaald scgseedfav
      121 ataettfavp amsttpsaav vsrkvpsktv aaavttmtlg rgaiprrpsg qplprrsmlp
      181 ppsqragrls vpsrltaprg rlsvaptaad ttfgankatp dgrlsapksa lqqpsqrqaa
      241 pvsqpataip gprvsslrrs vagasvgtaa aasrrslqap asgalascva krgpasrlsv
      301 ipataaasrt slkppakpaa tglrkslagp qktqtpagqp taqmakqapk pgsqpapqpk
      361 pkaalapqlp rsrlpppksr giggmprpap ssgvsssqpp prlllpives pvptrlsstp
      421 vrsdaplcgg pdltpirkr
//
LOCUS       XP_077501203             100 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111990 [Amblyomma americanum].
ACCESSION   XP_077501203
VERSION     XP_077501203.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645077.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526496) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..100
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..100
                     /product="uncharacterized protein LOC144111990"
                     /calculated_mol_wt=10569
     CDS             1..100
                     /gene="LOC144111990"
                     /coded_by="XM_077645077.1:4413..4715"
                     /db_xref="GeneID:144111990"
ORIGIN      
        1 mndtaknavc amfvaivygq clhasvsvsa tpldtvlfea ktkslakstl esklascrnf
       61 vkspqreeae daavgvtalg rthlkqrals spqefvplaf
//
LOCUS       XP_077501204             570 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111992 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501204
VERSION     XP_077501204.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645078.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526498) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..570
                     /product="uncharacterized protein LOC144111992 isoform X1"
                     /calculated_mol_wt=62731
     Region          106..>212
                     /region_name="SWIM"
                     /note="Uncharacterized protein, contains SWIM-type Zn
                     finger domain [Function unknown]; COG4715"
                     /db_xref="CDD:443750"
     Region          307..498
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(362,401,415,417)
                     /site_type="active"
                     /db_xref="CDD:411747"
     Region          521..561
                     /region_name="PHD_SF"
                     /note="PHD finger superfamily; cl22851"
                     /db_xref="CDD:473978"
     Site            order(521,532..535,539,556)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:276966"
     CDS             1..570
                     /gene="LOC144111992"
                     /coded_by="XM_077645078.1:123..1835"
                     /db_xref="GeneID:144111992"
ORIGIN      
        1 mssdyfkkld teaqvryrak lsfrgeelpd aldddvvrfa ftsgprnlpp vtvadiylyl
       61 vegvcfytne qfksykleda ynaficgkvk qvksfkagah gdgvvviaat veasqtlsks
      121 ykawcivked gtiesahctc maglgeccth vaallfrvea taqyglndps ptdvackwie
      181 askgstaapv seieffkpki grappqvsea pdysvpvltd eqvsrflhqv keiapntlil
      241 tsisdsedtd sapetaalep vrvgrckqls akpplaeiyq dldidegkkv eltaaccsei
      301 eeatrgqars prwlqerrgr itasvmhrva acstgaaglv aeimgyvktp qvsnlrwgse
      361 tekkavqafi etesqkhvdf slrdcglfia rsmpfigasp dalvscsccp ksvlevkcpa
      421 tmkdasltkg ltklaylned lqlkhnhayy tqiqaqmalt glcqayfvvf tgssltkeii
      481 sfdetfwaat klkaanffft yifpelqsmh ifsqiecakk tchcrgtklg hivecslcea
      541 tfhlkcvklr rtprqwicta cqhngqtade
//
LOCUS       XP_077501205             236 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111992 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501205
VERSION     XP_077501205.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645079.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526498) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..236
                     /product="uncharacterized protein LOC144111992 isoform X2"
                     /calculated_mol_wt=25822
     Region          106..>212
                     /region_name="SWIM"
                     /note="Uncharacterized protein, contains SWIM-type Zn
                     finger domain [Function unknown]; COG4715"
                     /db_xref="CDD:443750"
     CDS             1..236
                     /gene="LOC144111992"
                     /coded_by="XM_077645079.1:123..833"
                     /db_xref="GeneID:144111992"
ORIGIN      
        1 mssdyfkkld teaqvryrak lsfrgeelpd aldddvvrfa ftsgprnlpp vtvadiylyl
       61 vegvcfytne qfksykleda ynaficgkvk qvksfkagah gdgvvviaat veasqtlsks
      121 ykawcivked gtiesahctc maglgeccth vaallfrvea taqyglndps ptdvackwie
      181 askgstaapv seieffkpki grappqvsea pdysvpvltd eqvsrflhqv kvrtsh
//
LOCUS       XP_077501207             110 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111995 [Amblyomma americanum].
ACCESSION   XP_077501207
VERSION     XP_077501207.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645081.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526498) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..110
                     /product="uncharacterized protein LOC144111995"
                     /calculated_mol_wt=12032
     Region          37..>86
                     /region_name="BEN"
                     /note="The BEN domain is found in diverse animal proteins;
                     cl11158"
                     /db_xref="CDD:472128"
     CDS             1..110
                     /gene="LOC144111995"
                     /coded_by="XM_077645081.1:193..525"
                     /db_xref="GeneID:144111995"
ORIGIN      
        1 malsytfhfq idigggvlie etaleklrss cpgapakfar glmrqlftad elrgkslfgr
       61 ksnshpdasl realdpvrvn aiigftiref kadpirlkss lssllsreik
//
LOCUS       XP_077501208             119 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111996 [Amblyomma americanum].
ACCESSION   XP_077501208
VERSION     XP_077501208.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645082.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526498) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..119
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..119
                     /product="uncharacterized protein LOC144111996"
                     /calculated_mol_wt=12894
     Region          46..>95
                     /region_name="BEN"
                     /note="The BEN domain is found in diverse animal proteins;
                     cl11158"
                     /db_xref="CDD:472128"
     CDS             1..119
                     /gene="LOC144111996"
                     /coded_by="XM_077645082.1:28..387"
                     /db_xref="GeneID:144111996"
ORIGIN      
        1 mlddmksida isavrpcpqi digggvliee taleklrssc pgapakfarg lmrqlftade
       61 lrgkslfgrk snshpdsslr ealdpvrvna iigftirefk adpirlkssl ssllsreik
//
LOCUS       XP_077501209             255 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111998 [Amblyomma americanum].
ACCESSION   XP_077501209
VERSION     XP_077501209.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645083.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526498) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..255
                     /product="uncharacterized protein LOC144111998"
                     /calculated_mol_wt=28365
     CDS             1..255
                     /gene="LOC144111998"
                     /coded_by="XM_077645083.1:44..811"
                     /db_xref="GeneID:144111998"
ORIGIN      
        1 mrgknaaiqa ealeaqiskl pprqqeavrq cfaaskvkpn glrytkmwvl dciimkmksp
       61 rlyehlrrns ilslpckstl kcyvsayrtv fgfsdkvlrq lkvksaelda wkkhgglvkl
      121 sehfavktsg viegfvdlgp ftaakdkgvp cdhgmvilfv pfqgkwsqvi gyfathgnmk
      181 gdilakvvie atvlaeksgl fvdfitcdga swnrrmwril giqasaestt ckiqhpsdas
      241 rnlffisdfp hliks
//
LOCUS       XP_077501210             142 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111999 [Amblyomma americanum].
ACCESSION   XP_077501210
VERSION     XP_077501210.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645084.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526498) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 11% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..142
                     /product="uncharacterized protein LOC144111999"
                     /calculated_mol_wt=15903
     CDS             1..142
                     /gene="LOC144111999"
                     /coded_by="XM_077645084.1:118..546"
                     /db_xref="GeneID:144111999"
ORIGIN      
        1 mdhilikwtq gekwdvypir amvntavgfr lltepgaidd vrgtielftw kdseppapae
       61 vldigkarvl etkrarlasa glrdegdvmt eslecspnee fcsqcenmrr eldvvkrrne
      121 dleaahgmsd hhgavkgcyn ly
//
LOCUS       XP_077501212             360 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112000 [Amblyomma americanum].
ACCESSION   XP_077501212
VERSION     XP_077501212.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645086.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526498) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..360
                     /product="uncharacterized protein LOC144112000"
                     /calculated_mol_wt=39873
     Region          109..155
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          190..350
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..360
                     /gene="LOC144112000"
                     /coded_by="XM_077645086.1:1..1083"
                     /db_xref="GeneID:144112000"
ORIGIN      
        1 mavdaaqkdk scqtddiarc sgtfsvflcs argneartqv nqkivcdaev qckpevvshq
       61 sgrdsrtacf tgydsvqaca aamkdlcgvs pdiyalllsi mprsaertcd vpitdrlvlf
      121 lmklklgity sslavlfcvs rntvsrhfks vlktlsiatq kwiyrppdhv irqtipncfk
      181 vhyqdcsmii dcrevrteqp ytvqqqrvly shykggytlk flvgitpcga vcfgskaygg
      241 rlsdsfitvd sgfldlvqpg dvvmadkgfp gikagleevr avlvmppflq gqdqfsesev
      301 hetyqiaqvr ihveriiqci kmfnilntri pteliavmse vfhvccalan lqppiisseq
//
LOCUS       XP_077501213             358 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112001 [Amblyomma americanum].
ACCESSION   XP_077501213
VERSION     XP_077501213.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645087.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526498) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..358
                     /product="uncharacterized protein LOC144112001"
                     /calculated_mol_wt=39827
     Region          96..141
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          176..343
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..358
                     /gene="LOC144112001"
                     /coded_by="XM_077645087.1:103..1179"
                     /db_xref="GeneID:144112001"
ORIGIN      
        1 metetdlsmq nishlieeng rlkqrlasle eecsaakqka gelenkhtnh aiitkevvmq
       61 saktvkfytg fvstamftaf lqfvlsawkp scatwldaet qlimvlmrlr lglltqdlsc
      121 rfgisnatvs sifhawldvl aanmtkliiw psrqtlqanr paafqdpffd gvtgiidcte
      181 vfiqrpilmt arsqtyshyk hhntvkilvv ispsgaitfv skawggrtpd keitlrsgll
      241 dkvkegdvfl vdrgfkcgem faargathlm psltknraql pgaevttsrk vssvrihver
      301 aiqrlkvfri lqtvlplnfv krtgdknlat idkvvvvcsa lvnlhtpiik nsqrrsas
//
LOCUS       XP_077501214             161 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112002, partial [Amblyomma
            americanum].
ACCESSION   XP_077501214
VERSION     XP_077501214.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645088.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526498) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 14% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: incomplete on the carboxy end.
FEATURES             Location/Qualifiers
     source          1..161
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..>161
                     /product="uncharacterized protein LOC144112002"
     CDS             1..161
                     /gene="LOC144112002"
                     /coded_by="XM_077645088.1:47..>531"
                     /db_xref="GeneID:144112002"
ORIGIN      
        1 mdedsifwri frcflrseny fnrrgiydld ggdpgenara pepsneygrl irpnllrgpa
       61 ichrcflaeh lryynmvkrr mgrqeeaska egppaaaapa ngkrkttdes qstnvdnqek
      121 ykegqqeqqq qqhqqqqqqq qqlkqqqqlk qlkqqqqqqq q
//
LOCUS       XP_077501215             103 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112003 [Amblyomma americanum].
ACCESSION   XP_077501215
VERSION     XP_077501215.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645089.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526498) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 11% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..103
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..103
                     /product="uncharacterized protein LOC144112003"
                     /calculated_mol_wt=10085
     CDS             1..103
                     /gene="LOC144112003"
                     /coded_by="XM_077645089.1:56..367"
                     /db_xref="GeneID:144112003"
ORIGIN      
        1 miaavsgepv skgyvqsrss qslllfqeda dgasptgstl nqacshasgf imvqsdhtya
       61 gpsteasass lepgtagvaa tlsasadlsp galfssspls yeg
//
LOCUS       XP_077501216             483 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112008 [Amblyomma americanum].
ACCESSION   XP_077501216
VERSION     XP_077501216.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645090.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526498) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..483
                     /product="uncharacterized protein LOC144112008"
                     /calculated_mol_wt=52204
     Region          22..463
                     /region_name="AFD_class_I"
                     /note="Adenylate forming domain, Class I superfamily;
                     cl17068"
                     /db_xref="CDD:473059"
     Site            order(124,127..132,134..135)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341237"
     Site            order(127,243..244,266..271,349,361,364,375,463)
                     /site_type="other"
                     /note="AMP binding site [chemical binding]"
                     /db_xref="CDD:341237"
     Site            order(127,169..170,216,218..219,222,243..244,266..271,349,
                     361,364,372..375,444)
                     /site_type="active"
                     /db_xref="CDD:341237"
     Site            order(169,218..219,222,243,372..374,437,444)
                     /site_type="other"
                     /note="CoA binding site [chemical binding]"
                     /db_xref="CDD:341237"
     CDS             1..483
                     /gene="LOC144112008"
                     /coded_by="XM_077645090.1:1..1452"
                     /db_xref="GeneID:144112008"
ORIGIN      
        1 mrlpnlppke ptaeaalvtg mvdaksgsry tfgelldtcr rvaaglrrlg lqpgnvvafk
       61 aatssellva mcgtlfaggi talikviigi tgasggatsl sqlkqspldd cesrnlcpds
      121 vlaiiyssgt tgspkgvqlt hrnviaqvvm ygllgpsvfd kddivictap vmhiagfwvv
      181 fgnlghgckl illhtldigl ileaidrhkp tmltlyptfl akfeehplle hvdttsvtkl
      241 mtggslvsss vlkkitlklg lkgviqvygm telgsciays tpemddfrsa gkpgpfveik
      301 vveentrkal gpyqhgeicv kspgafksyl nnpkataety engfvrtgdl gyytpdgrfy
      361 vcsrlkdlik cmdqqvapae leellssdpe vrqvvvvgvp hpeygeaara fvvprrllqp
      421 gsheeqreaq rlrdlvagrl aahkhlyagv eflediprta ngknlrsslk eayvqlhckc
      481 vkk
//
LOCUS       XP_077501218             117 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112009 [Amblyomma americanum].
ACCESSION   XP_077501218
VERSION     XP_077501218.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645092.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526498) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..117
                     /product="uncharacterized protein LOC144112009"
                     /calculated_mol_wt=12819
     Region          <10..99
                     /region_name="AFD_class_I"
                     /note="Adenylate forming domain, Class I superfamily;
                     cl17068"
                     /db_xref="CDD:473059"
     CDS             1..117
                     /gene="LOC144112009"
                     /coded_by="XM_077645092.1:1..354"
                     /db_xref="GeneID:144112009"
ORIGIN      
        1 matslfiypg dvgyynpdgr ffvcgrlkel tkcidqqvap aeleellssd sgvlhvvvvg
       61 vphheygeaa rafvvprqrl qpgsleeqre aqrlqdlvaa lappalyvyc rrnsfgg
//
LOCUS       XP_077501219             755 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112012 [Amblyomma americanum].
ACCESSION   XP_077501219
VERSION     XP_077501219.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645093.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526498) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..755
                     /product="uncharacterized protein LOC144112012"
                     /calculated_mol_wt=84062
     Region          5..86
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..755
                     /gene="LOC144112012"
                     /coded_by="XM_077645093.1:848..3115"
                     /db_xref="GeneID:144112012"
ORIGIN      
        1 mptvclvrqc rttyengsnv rfhklpgdpq rralwlrain rdmgsqtgyv csehflpgdy
       61 etnldvlrsl gmdlknarik rdaiptqnls pgappkkrrr seelpepqdv eailtaeeaq
      121 tspqmvdsct qtprntftva cqtdgrdvrt rytqvnlhis vrkdihvqtd agqttsahce
      181 actntstapa sttqpvsslp pppcqtvpsl pidgrapspi sfaprqsssp ieseasdcee
      241 ehntlqssef fpslnetflg ldssteevlp qddkkfiife sclkelfqvc rkcfaacksv
      301 shvsgtwlqi qtlcanhhfl twssqpming kaagnvlmsa amlfagtspt svlrmfnyvn
      361 vqvftprtfy nyqrgyllpa idkiwqqqqe elfrqlegct vdlagdgrcd spgfcakflt
      421 yslhvaqlnk ilhfeqvqvg ecadvqssts mekfafvksl nmvkerglkv asvttdrhgq
      481 vtkymrtqep tirhyydcwh iskgikkkla ahakragcsv lkiwiqpasn hlywcaalsd
      541 gsaelltdmw ksmerhaani htghpglyth cahdelgere wlvpgttahn kfvevvsspr
      601 ilkdirqlap cthtfsleaf hsvligfapk svcfspegmr artqlailhf nenaskgqat
      661 taeglsrwki khpkarkgta vacpvkaept ydyvrlllke tvhcceqwps fkaafaenls
      721 tapppmsrsf prpsknelva arrsrfassm gsttv
//
LOCUS       XP_077501220             204 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112013 [Amblyomma americanum].
ACCESSION   XP_077501220
VERSION     XP_077501220.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645094.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526498) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..204
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..204
                     /product="uncharacterized protein LOC144112013"
                     /calculated_mol_wt=22585
     Region          <74..198
                     /region_name="P2RX7_C"
                     /note="P2X purinoreceptor 7 intracellular domain;
                     pfam20478"
                     /db_xref="CDD:466627"
     CDS             1..204
                     /gene="LOC144112013"
                     /coded_by="XM_077645094.1:16..630"
                     /db_xref="GeneID:144112013"
ORIGIN      
        1 madlpelqrr llersrllgf epygdtplrv pdqpssedds ggsdngcedg argqqlavva
       61 rgttatfgsp dreppepgrv gnthwcfcgr cvsmptales tccreipaat vkqpsgcite
      121 hphfytlcld evvltvalqm lldhglrveh tgryryisys qlahwlwgrl grhhrkvlpa
      181 cavcqirerf psegytgfqy aqgl
//
LOCUS       XP_077501222             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112020 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501222
VERSION     XP_077501222.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645096.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526501) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144112020 isoform X1"
                     /calculated_mol_wt=16660
     CDS             1..158
                     /gene="LOC144112020"
                     /coded_by="XM_077645096.1:419..895"
                     /db_xref="GeneID:144112020"
ORIGIN      
        1 msplsrfgav lvcacsayia ehniinrtma atcswfsghg kskktgasef kvekamydtv
       61 kgipmdavfh krtaipiwst eavvlcsssg kcsggdsgrq gkgivatqle sscyqgnsty
      121 stlpektham mlgasiltil pilstneakd afvqisks
//
LOCUS       XP_077501223             142 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112020 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501223
VERSION     XP_077501223.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645097.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526501) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..142
                     /product="uncharacterized protein LOC144112020 isoform X2"
                     /calculated_mol_wt=15372
     CDS             1..142
                     /gene="LOC144112020"
                     /coded_by="XM_077645097.1:419..847"
                     /db_xref="GeneID:144112020"
ORIGIN      
        1 msplsrfgav lvcacsayia ehniinrtma atcswfsghg kskktgasef kvekamydtv
       61 kgipmdavfh krtaipiwst eavvlcsssg kcsggdsgrh cgytagvkll sgkfnlfdfa
      121 rkdarydawg lhldylanfv yq
//
LOCUS       XP_077501224             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112021 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501224
VERSION     XP_077501224.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645098.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526501) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144112021 isoform X1"
                     /calculated_mol_wt=16644
     CDS             1..158
                     /gene="LOC144112021"
                     /coded_by="XM_077645098.1:10..486"
                     /db_xref="GeneID:144112021"
ORIGIN      
        1 msplsrfgav lvcacsayia ehniinrtma atcswfsghg kskktgasef kvekamydtv
       61 kgipmdavfh krtaipiwst eavvlssssg kcsggdsgrq gkgivatqle sscyqgnsty
      121 stlpektham mlgasiltil pilstneakd afvqisks
//
LOCUS       XP_077501225             142 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112021 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501225
VERSION     XP_077501225.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645099.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526501) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..142
                     /product="uncharacterized protein LOC144112021 isoform X2"
                     /calculated_mol_wt=15344
     CDS             1..142
                     /gene="LOC144112021"
                     /coded_by="XM_077645099.1:10..438"
                     /db_xref="GeneID:144112021"
ORIGIN      
        1 msplsrfgav lvcacsayia ehniinrtma atcswfsghg kskktgasef kvekamydtv
       61 kgipmdavfh krtaipiwst eavvlssssg kcsggdsgrh cgytagvkll sgkfnlfdfa
      121 rkdarydawg lhldytanfv yq
//
LOCUS       XP_077501226             121 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112024 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501226
VERSION     XP_077501226.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645100.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526501) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..121
                     /product="uncharacterized protein LOC144112024 isoform X1"
                     /calculated_mol_wt=12754
     CDS             1..121
                     /gene="LOC144112024"
                     /coded_by="XM_077645100.1:259..624"
                     /db_xref="GeneID:144112024"
ORIGIN      
        1 mrrvlvsttr ststiaaakq ekkavtgirr vfvssiinrt maatcswfsg hgkskktgas
       61 efkvekamyd tvkgipkdav fhkrtaipiw steavvlcss sgkcsggdsg rqgkgspqgs
      121 e
//
LOCUS       XP_077501227             109 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112024 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501227
VERSION     XP_077501227.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645101.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526501) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..109
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..109
                     /product="uncharacterized protein LOC144112024 isoform X2"
                     /calculated_mol_wt=11357
     CDS             1..109
                     /gene="LOC144112024"
                     /coded_by="XM_077645101.1:56..385"
                     /db_xref="GeneID:144112024"
ORIGIN      
        1 msplsrfgav lvcacsayia ehniinrtma atcswfsghg kskktgasef kvekamydtv
       61 kgipkdavfh krtaipiwst eavvlcsssg kcsggdsgrq gkgspqgse
//
LOCUS       XP_077501229             109 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112026 [Amblyomma americanum].
ACCESSION   XP_077501229
VERSION     XP_077501229.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645103.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526501) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 11% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..109
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..109
                     /product="uncharacterized protein LOC144112026"
                     /calculated_mol_wt=11344
     CDS             1..109
                     /gene="LOC144112026"
                     /coded_by="XM_077645103.1:420..749"
                     /db_xref="GeneID:144112026"
ORIGIN      
        1 msplsrfgav lvcacsayia ehniinrtma atcswfsghg kskktgasef kvekamydtv
       61 kgipmdavfh krtaipiwst eavvlssssg kcsggdsgrq gkgspqgse
//
LOCUS       XP_077501230             376 aa            linear   INV 25-NOV-2025
DEFINITION  protein phosphatase 1L isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501230
VERSION     XP_077501230.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645104.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526502) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..376
                     /product="protein phosphatase 1L isoform X1"
                     /calculated_mol_wt=41238
     Region          105..367
                     /region_name="PP2Cc"
                     /note="Serine/threonine phosphatases, family 2C, catalytic
                     domain; The protein architecture and deduced catalytic
                     mechanism of PP2C phosphatases are similar to the PP1,
                     PP2A, PP2B family of protein Ser/Thr phosphatases, with
                     which PP2C shares no sequence...; cd00143"
                     /db_xref="CDD:238083"
     Site            order(115,119..120,139..141,318,358)
                     /site_type="active"
                     /db_xref="CDD:238083"
     CDS             1..376
                     /gene="LOC144112028"
                     /coded_by="XM_077645104.1:138..1268"
                     /db_xref="GeneID:144112028"
ORIGIN      
        1 medeledelv qeifwahvsl vsraaradlr pmadaaafyl rlyvlrpgtl avfvallaav
       61 llcqstteaw lrlllrrcel rgdavkqmlr rgpperrswe akaehvgvfa lqgrrprmed
      121 rfsilsdhph nlhlygvfdg hggevasdya qqhlfaslmp klvaaakrrr pdepveqlla
      181 eftqlltdai lqldadllai lkerhdlsgs tllvalvygs rlvvanvgds rgvladlrgq
      241 alplsfdhkp qqlkehkrir eaggfitfng vwrvagvlat sralgdyplk erhllvaqpd
      301 vltfdlsega acavlasdgl wdalsneqaa alalahldep lhgarelalr aysagsldni
      361 talvvdlrna sakvng
//
LOCUS       XP_077501231             376 aa            linear   INV 25-NOV-2025
DEFINITION  protein phosphatase 1L isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501231
VERSION     XP_077501231.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645105.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526502) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..376
                     /product="protein phosphatase 1L isoform X1"
                     /calculated_mol_wt=41238
     Region          105..367
                     /region_name="PP2Cc"
                     /note="Serine/threonine phosphatases, family 2C, catalytic
                     domain; The protein architecture and deduced catalytic
                     mechanism of PP2C phosphatases are similar to the PP1,
                     PP2A, PP2B family of protein Ser/Thr phosphatases, with
                     which PP2C shares no sequence...; cd00143"
                     /db_xref="CDD:238083"
     Site            order(115,119..120,139..141,318,358)
                     /site_type="active"
                     /db_xref="CDD:238083"
     CDS             1..376
                     /gene="LOC144112028"
                     /coded_by="XM_077645105.1:138..1268"
                     /db_xref="GeneID:144112028"
ORIGIN      
        1 medeledelv qeifwahvsl vsraaradlr pmadaaafyl rlyvlrpgtl avfvallaav
       61 llcqstteaw lrlllrrcel rgdavkqmlr rgpperrswe akaehvgvfa lqgrrprmed
      121 rfsilsdhph nlhlygvfdg hggevasdya qqhlfaslmp klvaaakrrr pdepveqlla
      181 eftqlltdai lqldadllai lkerhdlsgs tllvalvygs rlvvanvgds rgvladlrgq
      241 alplsfdhkp qqlkehkrir eaggfitfng vwrvagvlat sralgdyplk erhllvaqpd
      301 vltfdlsega acavlasdgl wdalsneqaa alalahldep lhgarelalr aysagsldni
      361 talvvdlrna sakvng
//
LOCUS       XP_077501232             376 aa            linear   INV 25-NOV-2025
DEFINITION  protein phosphatase 1L isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501232
VERSION     XP_077501232.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645106.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526502) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..376
                     /product="protein phosphatase 1L isoform X1"
                     /calculated_mol_wt=41238
     Region          105..367
                     /region_name="PP2Cc"
                     /note="Serine/threonine phosphatases, family 2C, catalytic
                     domain; The protein architecture and deduced catalytic
                     mechanism of PP2C phosphatases are similar to the PP1,
                     PP2A, PP2B family of protein Ser/Thr phosphatases, with
                     which PP2C shares no sequence...; cd00143"
                     /db_xref="CDD:238083"
     Site            order(115,119..120,139..141,318,358)
                     /site_type="active"
                     /db_xref="CDD:238083"
     CDS             1..376
                     /gene="LOC144112028"
                     /coded_by="XM_077645106.1:138..1268"
                     /db_xref="GeneID:144112028"
ORIGIN      
        1 medeledelv qeifwahvsl vsraaradlr pmadaaafyl rlyvlrpgtl avfvallaav
       61 llcqstteaw lrlllrrcel rgdavkqmlr rgpperrswe akaehvgvfa lqgrrprmed
      121 rfsilsdhph nlhlygvfdg hggevasdya qqhlfaslmp klvaaakrrr pdepveqlla
      181 eftqlltdai lqldadllai lkerhdlsgs tllvalvygs rlvvanvgds rgvladlrgq
      241 alplsfdhkp qqlkehkrir eaggfitfng vwrvagvlat sralgdyplk erhllvaqpd
      301 vltfdlsega acavlasdgl wdalsneqaa alalahldep lhgarelalr aysagsldni
      361 talvvdlrna sakvng
//
LOCUS       XP_077501233             334 aa            linear   INV 25-NOV-2025
DEFINITION  protein phosphatase 1L isoform X2 [Amblyomma americanum].
ACCESSION   XP_077501233
VERSION     XP_077501233.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645107.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526502) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..334
                     /product="protein phosphatase 1L isoform X2"
                     /calculated_mol_wt=36501
     Region          104..325
                     /region_name="PP2Cc"
                     /note="Serine/threonine phosphatases, family 2C, catalytic
                     domain; The protein architecture and deduced catalytic
                     mechanism of PP2C phosphatases are similar to the PP1,
                     PP2A, PP2B family of protein Ser/Thr phosphatases, with
                     which PP2C shares no sequence...; cd00143"
                     /db_xref="CDD:238083"
     CDS             1..334
                     /gene="LOC144112028"
                     /coded_by="XM_077645107.1:138..1142"
                     /db_xref="GeneID:144112028"
ORIGIN      
        1 medeledelv qeifwahvsl vsraaradlr pmadaaafyl rlyvlrpgtl avfvallaav
       61 llcqstteaw lrlllrrcel rgdavkqmlr rgpperrswe akvasdyaqq hlfaslmpkl
      121 vaaakrrrpd epveqllaef tqlltdailq ldadllailk erhdlsgstl lvalvygsrl
      181 vvanvgdsrg vladlrgqal plsfdhkpqq lkehkrirea ggfitfngvw rvagvlatsr
      241 algdyplker hllvaqpdvl tfdlsegaac avlasdglwd alsneqaaal alahldeplh
      301 garelalray sagsldnita lvvdlrnasa kvng
//
LOCUS       XP_077501234             211 aa            linear   INV 25-NOV-2025
DEFINITION  elongation factor 1-beta-like [Amblyomma americanum].
ACCESSION   XP_077501234
VERSION     XP_077501234.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645108.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526502) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..211
                     /product="elongation factor 1-beta-like"
                     /calculated_mol_wt=23314
     Region          <3..63
                     /region_name="GST_C_eEF1b_like"
                     /note="Glutathione S-transferase C-terminal-like, alpha
                     helical domain of eukaryotic translation Elongation Factor
                     1 beta; cd10308"
                     /db_xref="CDD:198341"
     Site            order(19..23,51..52,54..55,59,62..63)
                     /site_type="other"
                     /note="putative protein interface 2 [polypeptide binding]"
                     /db_xref="CDD:198341"
     Region          90..>111
                     /region_name="EF-1_beta_acid"
                     /note="Eukaryotic elongation factor 1 beta central acidic
                     region; pfam10587"
                     /db_xref="CDD:463158"
     Region          128..211
                     /region_name="EF1_GNE"
                     /note="EF-1 guanine nucleotide exchange domain; pfam00736"
                     /db_xref="CDD:459919"
     CDS             1..211
                     /gene="LOC144112031"
                     /coded_by="XM_077645108.1:92..727"
                     /db_xref="GeneID:144112031"
ORIGIN      
        1 mafgdlktdt gvsklnehlv lrsyvegyqp sqadattysa lsgapsaekf phaarwyrhi
       61 gsysasersa fpgqkgepak ataddddvdl fgsddevdee aekarqervk ayaekkakkp
      121 gviakssvvl dvkpwddetd mkelerlvrt vtcdghvwgt sklvplaygi hklqivcvve
      181 dekvsidwli eeienfkdhv qsvdiaafqk i
//
LOCUS       XP_077501235             175 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112032 [Amblyomma americanum].
ACCESSION   XP_077501235
VERSION     XP_077501235.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645109.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526502) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..175
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..175
                     /product="uncharacterized protein LOC144112032"
                     /calculated_mol_wt=19807
     CDS             1..175
                     /gene="LOC144112032"
                     /coded_by="XM_077645109.1:72..599"
                     /db_xref="GeneID:144112032"
ORIGIN      
        1 mlserrfcld rgddpreias hikdlvlllq envasvvlvf kvlprhfdep rkaqfedrrr
       61 rqlnrrlsat lkrlsgvhil npehrfldas gkpmlslfaa dryhvardrg idqmskiiva
      121 alvkiygpgi apasrarpge vyvvhrcrrc gakghktdhc waycsprrha aagrg
//
LOCUS       XP_077501237             107 aa            linear   INV 25-NOV-2025
DEFINITION  small ubiquitin-related modifier-like [Amblyomma americanum].
ACCESSION   XP_077501237
VERSION     XP_077501237.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645111.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526502) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..107
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..107
                     /product="small ubiquitin-related modifier-like"
                     /calculated_mol_wt=11598
     Region          18..93
                     /region_name="Ubl_SUMO1"
                     /note="ubiquitin-like (Ubl) domain found in small
                     ubiquitin-related modifier 1 (SUMO-1) and similar
                     proteins; cd16114"
                     /db_xref="CDD:340531"
     Site            order(18,20,24,26,28,30,32..36,38..40,43..44,47,51,57..58,
                     60,62,65,67,86,88..93)
                     /site_type="other"
                     /note="SUMO1-SAE2-SAE1 complex [polypeptide binding]"
                     /db_xref="CDD:340531"
     Site            order(18,20,29..35,39..40,43..44,47,51..52)
                     /site_type="other"
                     /note="peptide binding site 1 [polypeptide binding]"
                     /db_xref="CDD:340531"
     Site            order(18,23,29,31..36,38..40,43..44,47..48,50..52)
                     /site_type="other"
                     /note="peptide binding site 2 [polypeptide binding]"
                     /db_xref="CDD:340531"
     Site            order(18,20,30..34,39,43..44,47,51)
                     /site_type="other"
                     /note="SUMO1-Ysmb-9 interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:340531"
     Site            order(22,24..26,63..64,78..80,82..84,86..88)
                     /site_type="other"
                     /note="SUMO1-UBC9 interaction site [polypeptide binding]"
                     /db_xref="CDD:340531"
     Site            order(26..36,39,43,47,51,58,60,89,91..93)
                     /site_type="other"
                     /note="SUMO1-E2I-RanBP2 interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:340531"
     Site            order(26..28,55,57..58,60,64..67,86,88..93)
                     /site_type="other"
                     /note="SUMO1-SAE2-SAE1-ATP-Mg complex [polypeptide
                     binding]"
                     /db_xref="CDD:340531"
     Site            order(26,57,60,62,64..65,67,72,76,86,88..93)
                     /site_type="other"
                     /note="SUMO1-SENP1-Rangap complex [polypeptide binding]"
                     /db_xref="CDD:340531"
     Site            order(45,51)
                     /site_type="other"
                     /note="key conserved lysines"
                     /db_xref="CDD:340531"
     Site            order(55,89..93)
                     /site_type="other"
                     /note="SUMO1-UBE2K interaction site [polypeptide binding]"
                     /db_xref="CDD:340531"
     Site            order(57,60,62,64..67,69,72,86,88..93)
                     /site_type="other"
                     /note="SUMO1-SENP2 interaction site [polypeptide binding]"
                     /db_xref="CDD:340531"
     Site            order(57,60,62,64..65,67,69,71..72,76..77,86,88..93)
                     /site_type="other"
                     /note="SUMO1-SENP1 interaction site [polypeptide binding]"
                     /db_xref="CDD:340531"
     CDS             1..107
                     /gene="LOC144112033"
                     /coded_by="XM_077645111.1:275..598"
                     /db_xref="GeneID:144112033"
ORIGIN      
        1 magsgqdpkp egsdsseyik lkvvgqdgne ihfkvkmttq mgklkksyse rvamsvsslr
       61 flfdgkrisd detpkqlemv nddvievyqe qtggsrvaav sgirpas
//
LOCUS       XP_077501238             153 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112034 [Amblyomma americanum].
ACCESSION   XP_077501238
VERSION     XP_077501238.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645112.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526502) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..153
                     /product="uncharacterized protein LOC144112034"
                     /calculated_mol_wt=15895
     CDS             1..153
                     /gene="LOC144112034"
                     /coded_by="XM_077645112.1:45..506"
                     /db_xref="GeneID:144112034"
ORIGIN      
        1 mhqclrsaqr rlfgdseagc svrpatafpw taggrwssar tvthwllfag llgtaatlml
       61 vvsgvrtgre tmaalaasqp rqpaaghgga tsghscaayr tclwfrhati qvplfnlllp
      121 asrwfsggdg flsaaasgva qepcgrfggd scs
//
LOCUS       XP_077501239             199 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112036 [Amblyomma americanum].
ACCESSION   XP_077501239
VERSION     XP_077501239.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645113.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526502) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..199
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..199
                     /product="uncharacterized protein LOC144112036"
                     /calculated_mol_wt=22354
     Region          <20..146
                     /region_name="TesA"
                     /note="Lysophospholipase L1 or related esterase. Includes
                     spore coat protein LipC/YcsK [Cell cycle control, cell
                     division, chromosome partitioning, Lipid transport and
                     metabolism]; COG2755"
                     /db_xref="CDD:442045"
     CDS             1..199
                     /gene="LOC144112036"
                     /coded_by="XM_077645113.1:1..600"
                     /db_xref="GeneID:144112036"
ORIGIN      
        1 mtskkrlgaa eiipetcfrh adfvvlyvgg ndldrgddrr eiashikdlv lllqenvasv
       61 vlvfkvlprh fdeprkaqfe drrrrqlnrr lsatlkrlsg vhilnpehrf ldasgkpmls
      121 lfaadryhva rdrgidqmsk iivaalvkiy gpgiasasra rpgevyvvhr crrcgakghk
      181 tdhcwaycsp rrhaaagrg
//
LOCUS       XP_077501240             171 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112037, partial [Amblyomma
            americanum].
ACCESSION   XP_077501240
VERSION     XP_077501240.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645114.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526502) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         <1..171
                     /product="uncharacterized protein LOC144112037"
     CDS             1..171
                     /gene="LOC144112037"
                     /coded_by="XM_077645114.1:<1..516"
                     /db_xref="GeneID:144112037"
ORIGIN      
        1 kfgiliwafs isvqrppssi tnasiaylag fvaraveerr tcsfcpalhq sdgspllmgl
       61 islqsrgglt yprselvsvl vaikkavdia lphikksnvr qqlaelllpr iqqcplfvcg
      121 aredhaastv svvfdkfvrp llsnvgatvt draayrkkla skplhrkvlr v
//
LOCUS       XP_077501241             515 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112039 [Amblyomma americanum].
ACCESSION   XP_077501241
VERSION     XP_077501241.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645115.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526502) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..515
                     /product="uncharacterized protein LOC144112039"
                     /calculated_mol_wt=58920
     Region          186..275
                     /region_name="SCAN"
                     /note="SCAN domain; pfam02023"
                     /db_xref="CDD:460417"
     Region          343..358
                     /region_name="zf-CCHC"
                     /note="Zinc knuckle; pfam00098"
                     /db_xref="CDD:395050"
     CDS             1..515
                     /gene="LOC144112039"
                     /coded_by="XM_077645115.1:1..1548"
                     /db_xref="GeneID:144112039"
ORIGIN      
        1 mdlmkllrsd llslcdelgv dvekmkkshi ctllletane etvsdtwell kneeterkre
       61 enerqrlaae reerelrrlq landqkklec essrgmtper vsqaesfpmd rlmqpykige
      121 dlgyylvnfe rtcekrgyak etwpqkllmv lpceaativa rldakdandy ekvkasllrk
      181 yrlsaeefrq rfrgaskrss esyaefafsl kanltewlkg aeaydtkdkv vecvgleqfy
      241 nsipeavklw vqdrenvstv ekaaeladey atrrklsses sesekkafgl rkrfipknrs
      301 qfrnretveg tkqapekted rakqagaqka ltkefearkp frcfncqetg hiaarctktr
      361 lafsyandsd enlellrpyi helqvngklc rvlrdsgatm divhpsyvee edftgevawi
      421 rqvlenqsvc lpiarvvisg pfgelvteaa vssavslqyp ylfsnksnql lcdrgqklae
      481 gtvkaltrsk srelasklky sqeseatevw gevds
//
LOCUS       XP_077501242             203 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112040 [Amblyomma americanum].
ACCESSION   XP_077501242
VERSION     XP_077501242.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645116.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526502) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..203
                     /product="uncharacterized protein LOC144112040"
                     /calculated_mol_wt=21083
     CDS             1..203
                     /gene="LOC144112040"
                     /coded_by="XM_077645116.1:625..1236"
                     /db_xref="GeneID:144112040"
ORIGIN      
        1 mlgqpkqksk eedarkkrdr hktgggsaqc tvsaqseqvi avashimtrv gnqtdsdggi
       61 dlppvaslpv irllqpmvdg agneefhyae ddwepplvde pqspaaavgk sgatpeaieq
      121 esnlffpavq padasstaaa gcssatvasg aaagpttaea vatgdagrap rgrmallers
      181 ladeddyraa llreehelre ktp
//
LOCUS       XP_077501244              85 aa            linear   INV 25-NOV-2025
DEFINITION  SRA stem-loop-interacting RNA-binding protein, mitochondrial-like
            [Amblyomma americanum].
ACCESSION   XP_077501244
VERSION     XP_077501244.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645118.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526502) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..85
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..85
                     /product="SRA stem-loop-interacting RNA-binding protein,
                     mitochondrial-like"
                     /calculated_mol_wt=9203
     Region          8..80
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..85
                     /gene="LOC144112043"
                     /coded_by="XM_077645118.1:144..401"
                     /db_xref="GeneID:144112043"
ORIGIN      
        1 masrsalrvf vanlpwtvgr kelrehfsqy gyvassnvvf dkktgmskgf gfvtfgtrdg
       61 fvsatkhqqh vlegsslvvh pasns
//
LOCUS       XP_077501245             245 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112044 [Amblyomma americanum].
ACCESSION   XP_077501245
VERSION     XP_077501245.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645119.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526502) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..245
                     /product="uncharacterized protein LOC144112044"
                     /calculated_mol_wt=27118
     Region          76..150
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..245
                     /gene="LOC144112044"
                     /coded_by="XM_077645119.1:134..871"
                     /db_xref="GeneID:144112044"
ORIGIN      
        1 mlllrsfrrp ctsllchrsv rfaprctaaa vfsvlsfpsa cgqcavffrl ctlrvlllkr
       61 lalhfkmslr hvkkrcsvvh cnsvdsttgv klhrfpldfh rcrawvqfcr nvslekkaps
      121 qlrelricsr hfepsvylts grlrhdalpt rasiadtsse cssdmncsqs lsetsasvrr
      181 agepaavsge pvskgyvqsr ssqslllfqe dadgarptgs tlnqacshas gfimvqsdht
      241 crtkh
//
LOCUS       XP_077501246             428 aa            linear   INV 25-NOV-2025
DEFINITION  growth/differentiation factor 8-like [Amblyomma americanum].
ACCESSION   XP_077501246
VERSION     XP_077501246.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645120.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526506) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..428
                     /product="growth/differentiation factor 8-like"
                     /calculated_mol_wt=47065
     Region          80..298
                     /region_name="TGFb_propeptide"
                     /note="TGF-beta propeptide; cl46657"
                     /db_xref="CDD:480997"
     Region          323..427
                     /region_name="TGF_beta_INHB"
                     /note="transforming growth factor beta (TGF-beta) like
                     domain found in inhibin beta A chain (INHBA), B chain
                     (INHBB), C chain (INHBC), E chain (INHBE) and similar
                     proteins; cd13752"
                     /db_xref="CDD:381630"
     Site            order(391..392,394,415,418..419)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:381630"
     CDS             1..428
                     /gene="LOC144112047"
                     /coded_by="XM_077645120.1:730..2016"
                     /db_xref="GeneID:144112047"
ORIGIN      
        1 mhrhenqlpc hgtlsecvfc cvfltafssa vvlnsppvay navggsrhhp atvytssacd
       61 dcaarklaeq gnseeyryar ielikqqilk klrlreppnv riprsvlpsv ladkevllsq
      121 tgaddggekn srrqddfygt mqtavifpqn dshecasrgp hrgscfvfhl sseltsrsva
      181 hadlwvfkdl dpaassaavn htfvvselsv aeshrsnrhh qpmlrkrvva fessssvpew
      241 lrfdltslva qwagrrsrpr gleiscktcg slrnvapsps spgtpigstg dkqpflvvrt
      301 dpnasgssrs rrsvdcnasi srccresfyv sfkeigwdsw iiqpsgysan fcrgscihdi
      361 svnkyhhttv lqrflhssaa aginntlkls lcctpsrltp islifidddq vlkqkslpnm
      421 vveaceca
//
LOCUS       XP_077501248             281 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112048 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501248
VERSION     XP_077501248.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645122.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526509) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..281
                     /product="uncharacterized protein LOC144112048 isoform X1"
                     /calculated_mol_wt=30951
     Region          15..97
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..281
                     /gene="LOC144112048"
                     /coded_by="XM_077645122.1:1532..2377"
                     /db_xref="GeneID:144112048"
ORIGIN      
        1 mrrqppship ssprprakvl fliqeyknfk sdpskrlktk rqlrenladl vnskfgchlt
       61 hsqienkwrn lerkykmvrq hnsqsgadrr tcefeeelae ifekahsinp pyllgpglvr
      121 svqdsqaepa aspqpgpsrv tataspipgs sqpstsrgst tagdsqppsp saqpsplqqq
      181 pgparkrprt dtsgavlesa vahlevaeak rqqrhrehma legrkvaaee grtaalerva
      241 ealdrrelvs lcspihapah spihspirsp vrspsppyyn a
//
LOCUS       XP_077501249             280 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112048 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501249
VERSION     XP_077501249.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645123.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526509) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..280
                     /product="uncharacterized protein LOC144112048 isoform X2"
                     /calculated_mol_wt=30864
     Region          15..97
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..280
                     /gene="LOC144112048"
                     /coded_by="XM_077645123.1:1620..2462"
                     /db_xref="GeneID:144112048"
ORIGIN      
        1 mrrqppship ssprprakvl fliqeyknfk sdpskrlktk rqlrenladl vnskfgchlt
       61 hsqienkwrn lerkykmvrq hnsqsgadrr tcefeeelae ifekahsinp pyllgpglvr
      121 svqdqaepaa spqpgpsrvt ataspipgss qpstsrgstt agdsqppsps aqpsplqqqp
      181 gparkrprtd tsgavlesav ahlevaeakr qqrhrehmal egrkvaaeeg rtaalervae
      241 aldrrelvsl cspihapahs pihspirspv rspsppyyna
//
LOCUS       XP_077501250             294 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112049 [Amblyomma americanum].
ACCESSION   XP_077501250
VERSION     XP_077501250.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645124.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526509) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..294
                     /product="uncharacterized protein LOC144112049"
                     /calculated_mol_wt=32418
     CDS             1..294
                     /gene="LOC144112049"
                     /coded_by="XM_077645124.1:394..1278"
                     /db_xref="GeneID:144112049"
ORIGIN      
        1 meteaalriw krtdlyatpl kyttflsdgd rkayaavael diygsvpvqk edctnhvakk
       61 lgtalrkakf pggeklkdkt iaklqgyfqv aitsnrgnvr dmycaawasy fhscsrdgas
      121 shkfcpdgem swckhkqala lgqpapvhtp ilsvsqgkav lpiykrltde kllqrcvkgq
      181 tqnaaeslns kiwllcpktk fatrtvveta taiavlwfnk ghtgfeevlq elgilpskal
      241 lslskevdkr rissmstkat aearahhrnv tkkarleepa rrdcegptyg agaf
//
LOCUS       XP_077501251             767 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112051 [Amblyomma americanum].
ACCESSION   XP_077501251
VERSION     XP_077501251.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645125.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526509) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 47% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..767
                     /product="uncharacterized protein LOC144112051"
                     /calculated_mol_wt=87077
     Region          78..167
                     /region_name="SCAN"
                     /note="SCAN domain; pfam02023"
                     /db_xref="CDD:460417"
     Region          231..247
                     /region_name="ZnF_C2HC"
                     /note="zinc finger; smart00343"
                     /db_xref="CDD:197667"
     Region          705..>763
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     CDS             1..767
                     /gene="LOC144112051"
                     /coded_by="XM_077645125.1:39..2342"
                     /db_xref="GeneID:144112051"
ORIGIN      
        1 mkdlmqpyrk gediglflvn fertcekagf tratwpqcll tllsgeaanv iarlckedsd
       61 nydkvkssll kkyrmsaeaf rqkfrnaekq rdesypdfay klmanlgewl keakaydese
      121 kmmqcfgleq fyrrlpedir hwvqdrqdvt tvskaadlae efvsrraldr kgspkkeyqn
      181 rkasgergql fkvkeqarsv essekgagik eeapeaeerq kkafkkrrap vcyncqkpgh
      241 maavcknpkv vclsvdsnee nlkllepyir dlvvngkpcr vlrdsaatmd vvhpsyvepg
      301 qftgecawik qaveansvcl pianisiegp fgvlhteaav saslpmqypy lfsnrsdqll
      361 rqkdlvfgeg tvyaltrska rkiaakampl easssvgtve seppdasgkv ratateevni
      421 lgrpessknc melkeasvdw daclpatmfa lrtasheatg ftpaelvygr slrsplrilr
      481 eswegrgddp tvveyvlall erlhkaqeqv gremskaqvk akhyydrtar srcfeagdev
      541 mllrpskknk levqwegpak viqrlsdtny iinmpgkrkv qqiyhtnllk pyrerekivn
      601 mslnvpeetp ldfpelgapk negstdlaik amveatelsv dqnrdltelv refkdmfsdk
      661 pgrttmivhd ieltssqpvr skayrvsprq reimeaeikr mldldiieac esdytsplil
      721 vevpgkeprp cidyrrlnli tkdqtypipn ieeriekwge atvdhlr
//
LOCUS       XP_077501252             456 aa            linear   INV 25-NOV-2025
DEFINITION  TBC1 domain family member 13 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501252
VERSION     XP_077501252.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645126.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526509) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..456
                     /product="TBC1 domain family member 13 isoform X1"
                     /calculated_mol_wt=52479
     Region          <245..418
                     /region_name="TBC"
                     /note="Domain in Tre-2, BUB2p, and Cdc16p. Probable
                     Rab-GAPs; smart00164"
                     /db_xref="CDD:214540"
     CDS             1..456
                     /gene="LOC144112053"
                     /coded_by="XM_077645126.1:209..1579"
                     /db_xref="GeneID:144112053"
ORIGIN      
        1 mstyrtrvhd lqrlleseqi dmkrlrelcf qgipddsglr plcwrlllgf lppnrsewpa
       61 vlakqrslye qfvddmivsn qeggtsptep ddhplpwyhp aqrntatcwt alpintapll
      121 phprhapptv atktgcpvgk sgcfpsatet siawplsmsp ssrwqayfrd nevllqidkd
      181 vrrlcpdmcf fqrrtehpct riaadprvqg lrervqrsml haanvtrsrq gitnisacvr
      241 arplhepleq lgegqeahwe vverilflya klnpglgyvq gmneiigpiy ytlvadpnpe
      301 wrkyaeadcf fcftslmsei rdffiktlde sssgigamme rlmqllrrrd drlyghlkql
      361 qvepqyysfr wimlllsqdf plpdvlriwd slfadtkrfn fliyicyaml sklrdklmmg
      421 dfpsnikllq nfpdvdvsel iaqalsaqqe dlmsss
//
LOCUS       XP_077501253             427 aa            linear   INV 25-NOV-2025
DEFINITION  TBC1 domain family member 13 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077501253
VERSION     XP_077501253.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645127.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526509) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..427
                     /product="TBC1 domain family member 13 isoform X2"
                     /calculated_mol_wt=48832
     Region          124..389
                     /region_name="TBC"
                     /note="Domain in Tre-2, BUB2p, and Cdc16p. Probable
                     Rab-GAPs; smart00164"
                     /db_xref="CDD:214540"
     CDS             1..427
                     /gene="LOC144112053"
                     /coded_by="XM_077645127.1:2653..3936"
                     /db_xref="GeneID:144112053"
ORIGIN      
        1 mtgipddsgl rplcwrlllg flppnrsewp avlakqrsly eqfvddmivs nqeggtspte
       61 pddhplpwyh paqrntatcw talpintapl lphprhappt vatktgcpvg ksgcfpsate
      121 tsiawplsms pssrwqayfr dnevllqidk dvrrlcpdmc ffqrrtehpc triaadprvq
      181 glrervqrsm lhaanvtrsr qgitnisacv rarplheple qlgegqeahw evverilfly
      241 aklnpglgyv qgmneiigpi yytlvadpnp ewrkyaeadc ffcftslmse irdffiktld
      301 esssgigamm erlmqllrrr ddrlyghlkq lqvepqyysf rwimlllsqd fplpdvlriw
      361 dslfadtkrf nfliyicyam lsklrdklmm gdfpsnikll qnfpdvdvse liaqalsaqq
      421 edlmsss
//
LOCUS       XP_077501255             395 aa            linear   INV 25-NOV-2025
DEFINITION  TBC1 domain family member 13 isoform X3 [Amblyomma americanum].
ACCESSION   XP_077501255
VERSION     XP_077501255.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645129.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526509) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..395
                     /product="TBC1 domain family member 13 isoform X3"
                     /calculated_mol_wt=45978
     Region          31..357
                     /region_name="TBC"
                     /note="Domain in Tre-2, BUB2p, and Cdc16p. Probable
                     Rab-GAPs; smart00164"
                     /db_xref="CDD:214540"
     CDS             1..395
                     /gene="LOC144112053"
                     /coded_by="XM_077645129.1:217..1404"
                     /db_xref="GeneID:144112053"
ORIGIN      
        1 mstyrtrvhd lqrlleseqi dmkrlrelcf qgipddsglr plcwrlllgf lppnrsewpa
       61 vlakqrslye qfvddmivsn qeggtsptep ddhplsmsps srwqayfrdn evllqidkdv
      121 rrlcpdmcff qrrtehpctr iaadprvqgl rervqrsmlh aanvtrsrqg itnisacvra
      181 rplhepleql gegqeahwev verilflyak lnpglgyvqg mneiigpiyy tlvadpnpew
      241 rkyaeadcff cftslmseir dffiktldes ssgigammer lmqllrrrdd rlyghlkqlq
      301 vepqyysfrw imlllsqdfp lpdvlriwds lfadtkrfnf liyicyamls klrdklmmgd
      361 fpsnikllqn fpdvdvseli aqalsaqqed lmsss
//
LOCUS       XP_077501256             381 aa            linear   INV 25-NOV-2025
DEFINITION  TBC1 domain family member 13 isoform X4 [Amblyomma americanum].
ACCESSION   XP_077501256
VERSION     XP_077501256.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645130.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526509) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..381
                     /product="TBC1 domain family member 13 isoform X4"
                     /calculated_mol_wt=43548
     Region          78..343
                     /region_name="TBC"
                     /note="Domain in Tre-2, BUB2p, and Cdc16p. Probable
                     Rab-GAPs; smart00164"
                     /db_xref="CDD:214540"
     CDS             1..381
                     /gene="LOC144112053"
                     /coded_by="XM_077645130.1:230..1375"
                     /db_xref="GeneID:144112053"
ORIGIN      
        1 mivsnqeggt sptepddhpl pwyhpaqrnt atcwtalpin tapllphprh apptvatktg
       61 cpvgksgcfp satetsiawp lsmspssrwq ayfrdnevll qidkdvrrlc pdmcffqrrt
      121 ehpctriaad prvqglrerv qrsmlhaanv trsrqgitni sacvrarplh epleqlgegq
      181 eahwevveri lflyaklnpg lgyvqgmnei igpiyytlva dpnpewrkya eadcffcfts
      241 lmseirdffi ktldesssgi gammerlmql lrrrddrlyg hlkqlqvepq yysfrwimll
      301 lsqdfplpdv lriwdslfad tkrfnfliyi cyamlsklrd klmmgdfpsn ikllqnfpdv
      361 dvseliaqal saqqedlmss s
//
LOCUS       XP_077501257             366 aa            linear   INV 25-NOV-2025
DEFINITION  TBC1 domain family member 13 isoform X5 [Amblyomma americanum].
ACCESSION   XP_077501257
VERSION     XP_077501257.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645131.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526509) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..366
                     /product="TBC1 domain family member 13 isoform X5"
                     /calculated_mol_wt=42332
     Region          3..328
                     /region_name="TBC"
                     /note="Domain in Tre-2, BUB2p, and Cdc16p. Probable
                     Rab-GAPs; smart00164"
                     /db_xref="CDD:214540"
     CDS             1..366
                     /gene="LOC144112053"
                     /coded_by="XM_077645131.1:2666..3766"
                     /db_xref="GeneID:144112053"
ORIGIN      
        1 mtgipddsgl rplcwrlllg flppnrsewp avlakqrsly eqfvddmivs nqeggtspte
       61 pddhplsmsp ssrwqayfrd nevllqidkd vrrlcpdmcf fqrrtehpct riaadprvqg
      121 lrervqrsml haanvtrsrq gitnisacvr arplhepleq lgegqeahwe vverilflya
      181 klnpglgyvq gmneiigpiy ytlvadpnpe wrkyaeadcf fcftslmsei rdffiktlde
      241 sssgigamme rlmqllrrrd drlyghlkql qvepqyysfr wimlllsqdf plpdvlriwd
      301 slfadtkrfn fliyicyaml sklrdklmmg dfpsnikllq nfpdvdvsel iaqalsaqqe
      361 dlmsss
//
LOCUS       XP_077501258             134 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112054 [Amblyomma americanum].
ACCESSION   XP_077501258
VERSION     XP_077501258.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645132.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526509) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..134
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..134
                     /product="uncharacterized protein LOC144112054"
                     /calculated_mol_wt=14731
     Region          <37..86
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..134
                     /gene="LOC144112054"
                     /coded_by="XM_077645132.1:904..1308"
                     /db_xref="GeneID:144112054"
ORIGIN      
        1 mrrripfgrr clsvaavaaa avpyrqsttn wqpwmtafnn ahsrqrvvve gslgllkarf
       61 qrllyidvar isqavhivla acvlhnkarr rgdamemlee vdsgtnvspt epadddeeca
      121 vsagtlrdsv aqrl
//
LOCUS       XP_077501260             203 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-(1,3)-fucosyltransferase C-like [Amblyomma americanum].
ACCESSION   XP_077501260
VERSION     XP_077501260.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645134.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526510) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 34% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..203
                     /product="alpha-(1,3)-fucosyltransferase C-like"
                     /calculated_mol_wt=23557
     Region          41..203
                     /region_name="Glyco_transf_10"
                     /note="Glycosyltransferase family 10 (fucosyltransferase)
                     C-term; pfam00852"
                     /db_xref="CDD:459962"
     CDS             1..203
                     /gene="LOC144112056"
                     /coded_by="XM_077645134.1:1..612"
                     /db_xref="GeneID:144112056"
ORIGIN      
        1 mfllgrndav aldsdvldty gevkvkkngh hffkrtlkrl wqgkfkmadw aasscntsgt
       61 rehfvhelrk hiyvdvygkc gdqncsrsia cyrslaksyy ylsfensick dcvteklfkv
      121 lqydivpvvl grvsysraap agsfmdalsf kfpkdlalyi knvasdfily ssffkwkgmy
      181 qvkywtyytf cllcrklhsk sfr
//
LOCUS       XP_077501261             131 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112057 [Amblyomma americanum].
ACCESSION   XP_077501261
VERSION     XP_077501261.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645135.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526512) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..131
                     /product="uncharacterized protein LOC144112057"
                     /calculated_mol_wt=14142
     CDS             1..131
                     /gene="LOC144112057"
                     /coded_by="XM_077645135.1:82..477"
                     /db_xref="GeneID:144112057"
ORIGIN      
        1 mvlradavpv vhparrvpla lrdplrqele rmekaaiikk vdeptewfvp gkdllladml
       61 srapvptqas sasseadcdi havqvvssik maalrancck adasalpcln ttripvsqce
      121 sianlrgerf n
//
LOCUS       XP_077501262             443 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112058 [Amblyomma americanum].
ACCESSION   XP_077501262
VERSION     XP_077501262.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645136.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526512) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 41% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="uncharacterized protein LOC144112058"
                     /calculated_mol_wt=48238
     Region          <180..226
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(180..183,187..190)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133137"
     CDS             1..443
                     /gene="LOC144112058"
                     /coded_by="XM_077645136.1:83..1414"
                     /db_xref="GeneID:144112058"
ORIGIN      
        1 mvlradavpv vhparrvpla lrdplrqele rmekaaiikk vdeptewfvp gkdllladml
       61 srapvptqas sasseadcdi havqvvssiv stpmkerlek eirddpylse kmaalrancc
      121 kadasalpcl nttripvsqc esianlrger seaaktaill saageealdv ynnfsfeavl
      181 kayngsaikh vrvdreilvl ngvahvvtff vvkkgrqail glqtcqqfgl gpeavdsvew
      241 netapekafp dlfqgtgcvg rqyrmvlrad avpvvhparr vplalrdplr qdlermekaa
      301 iikkvdepte wfvpgkdlll admlsrapvp tqassassea dcdihavqvv ssivstpmke
      361 rlekeirddp ylsekmaalr ancckadasa lpclnttrip vsqcesianl rgerseaakt
      421 aillsaagee aldvynnfsf eag
//
LOCUS       XP_077501264             168 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112059 [Amblyomma americanum].
ACCESSION   XP_077501264
VERSION     XP_077501264.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645138.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526512) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 28% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..168
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..168
                     /product="uncharacterized protein LOC144112059"
                     /calculated_mol_wt=18155
     CDS             1..168
                     /gene="LOC144112059"
                     /coded_by="XM_077645138.1:83..589"
                     /db_xref="GeneID:144112059"
ORIGIN      
        1 mvlradavpv vhparrvpla lrdplrqele rmekaaiikk vdeptewfvp gkdllladml
       61 srapvptqas sasseadcdi havqvvssiv stpmkerlek eirddpylse kmaalrancc
      121 kadasalpcl kttripvsqs eaaktaills aageealdvy nnfsfeag
//
LOCUS       XP_077501265             168 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112060 [Amblyomma americanum].
ACCESSION   XP_077501265
VERSION     XP_077501265.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645139.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526512) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 28% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..168
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..168
                     /product="uncharacterized protein LOC144112060"
                     /calculated_mol_wt=18155
     CDS             1..168
                     /gene="LOC144112060"
                     /coded_by="XM_077645139.1:61..567"
                     /db_xref="GeneID:144112060"
ORIGIN      
        1 mvlradavpv vhparrvpla lrdplrqele rmekaaiikk vdeptewfvp gkdllladml
       61 srapvptqas sasseadcdi havqvvssiv stpmkerlek eirddpylse kmaalrancc
      121 kadasalpcl kttripvsqs eaaktaills aageealdvy nnfsfeag
//
LOCUS       XP_077501266             168 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112061 [Amblyomma americanum].
ACCESSION   XP_077501266
VERSION     XP_077501266.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645140.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526512) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 27% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..168
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..168
                     /product="uncharacterized protein LOC144112061"
                     /calculated_mol_wt=18155
     CDS             1..168
                     /gene="LOC144112061"
                     /coded_by="XM_077645140.1:83..589"
                     /db_xref="GeneID:144112061"
ORIGIN      
        1 mvlradavpv vhparrvpla lrdplrqele rmekaaiikk vdeptewfvp gkdllladml
       61 srapvptqas sasseadcdi havqvvssiv stpmkerlek eirddpylse kmaalrancc
      121 kadasalpcl kttripvsqs eaaktaills aageealdvy nnfsfeag
//
LOCUS       XP_077501267             179 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112062 [Amblyomma americanum].
ACCESSION   XP_077501267
VERSION     XP_077501267.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645141.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526512) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 27% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..179
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..179
                     /product="uncharacterized protein LOC144112062"
                     /calculated_mol_wt=19384
     CDS             1..179
                     /gene="LOC144112062"
                     /coded_by="XM_077645141.1:83..622"
                     /db_xref="GeneID:144112062"
ORIGIN      
        1 mvlradavpv vhparrvpla lrdplrqele rmekaaiikk vdeptewfvp gkdllladml
       61 srapvptqas sasseadcdi havqvvssiv stpmkerlek eirddpylse kmaalrancc
      121 kadasalpcl kttripvsqc esianlrger seaaktaill saageealdv ynnfsfeag
//
LOCUS       XP_077501268             214 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112064 [Amblyomma americanum].
ACCESSION   XP_077501268
VERSION     XP_077501268.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645142.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526514) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 36% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..214
                     /product="uncharacterized protein LOC144112064"
                     /calculated_mol_wt=24359
     CDS             1..214
                     /gene="LOC144112064"
                     /coded_by="XM_077645142.1:4..648"
                     /db_xref="GeneID:144112064"
ORIGIN      
        1 mpfakffggt eneaanieav vsthsttads sfnfkrkirn vmfilvavli lwknldlyfe
       61 ilstffsmvm ttgivviltv alvwfklllr ipnwpmrlld ehihqqaiid ttantggsge
      121 rnldyqndhk dkddlhdldg qehqedlgdl sdledldsqd desdecdqgy ltdvddqddm
      181 edfdnlfrhh gfllaiaesd dnglfveqnv elhf
//
LOCUS       XP_077501269             165 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112065 [Amblyomma americanum].
ACCESSION   XP_077501269
VERSION     XP_077501269.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645143.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526514) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..165
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..165
                     /product="uncharacterized protein LOC144112065"
                     /calculated_mol_wt=17988
     CDS             1..165
                     /gene="LOC144112065"
                     /coded_by="XM_077645143.1:53..550"
                     /db_xref="GeneID:144112065"
ORIGIN      
        1 mpsarltfsr tesetaiiea iestessatk gwfnskfkkm aayviyvvaa ililwqirgl
       61 hlnapsasgy vvlgiqiffa itlvlacvkl alglrefhsq lhneeyipsk dpesgasadg
      121 gggderddfe dfcrlqgfpl pgqpksdaes ddeesgempq vqlrf
//
LOCUS       XP_077501270             382 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112067 [Amblyomma americanum].
ACCESSION   XP_077501270
VERSION     XP_077501270.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645144.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526514) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..382
                     /product="uncharacterized protein LOC144112067"
                     /calculated_mol_wt=40579
     Region          300..371
                     /region_name="IL17"
                     /note="Interleukin-17; pfam06083"
                     /db_xref="CDD:461823"
     CDS             1..382
                     /gene="LOC144112067"
                     /coded_by="XM_077645144.1:409..1557"
                     /db_xref="GeneID:144112067"
ORIGIN      
        1 mlalcillpl vltggrcaav dgidkgpdna tvpdpspgpg stagdvggsr tasarfsval
       61 paspeynvsg sepgwatdlv gepvaeiilv rhgasdfate tdvtesinee anvnrnpnwl
      121 laylkkkara aamlqdgksn gtaaaaradd mrsqatavsl saqaadgtse apgqdvalaa
      181 avparteatq dcagkarrrs qrrrsgaerq vlplprmptr kaasrksgrs alrkvkvvls
      241 rgmrtvrtsr gnrtsptstp pspllppplv srtpwadgdt ltlrergclp letspavpdr
      301 stcpfrfits fdrrrlprll tsvrcmcpgs pcnarrgyrc leiskpvslm rrvgrmyvdq
      361 leempvacvc ayvgprvrgr sp
//
LOCUS       XP_077501271             108 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112068 [Amblyomma americanum].
ACCESSION   XP_077501271
VERSION     XP_077501271.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645145.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526514) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..108
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..108
                     /product="uncharacterized protein LOC144112068"
                     /calculated_mol_wt=12193
     CDS             1..108
                     /gene="LOC144112068"
                     /coded_by="XM_077645145.1:7..333"
                     /db_xref="GeneID:144112068"
ORIGIN      
        1 mpfakffgrt eneaanieav vsthsttags sfnfkrkirn vmlivvavlv lwknldlyfe
       61 vistgismlm mpagivlilt valpwfkllq ripnwhvrll dededeim
//
LOCUS       XP_077501273             214 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112070 [Amblyomma americanum].
ACCESSION   XP_077501273
VERSION     XP_077501273.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645147.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526514) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..214
                     /product="uncharacterized protein LOC144112070"
                     /calculated_mol_wt=24373
     CDS             1..214
                     /gene="LOC144112070"
                     /coded_by="XM_077645147.1:1..645"
                     /db_xref="GeneID:144112070"
ORIGIN      
        1 mpfakffgrt eneaanieav vsthsttads sfnfkrkirn vmfilvavli lwknldlyfe
       61 ilstffsvvm ttgivviltv alvwfklllr ipnwpvrlld ehihqqaiid ttantggsge
      121 gnldyqndrk dkddlhdlds qehqddlgdp sdledldyqd desdqcdqgy ltdgddqddm
      181 edfdnlfrhh gfllpiaesd dnglfveqnv elhf
//
LOCUS       XP_077501274             165 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112071 [Amblyomma americanum].
ACCESSION   XP_077501274
VERSION     XP_077501274.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645148.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526514) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..165
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..165
                     /product="uncharacterized protein LOC144112071"
                     /calculated_mol_wt=18188
     CDS             1..165
                     /gene="LOC144112071"
                     /coded_by="XM_077645148.1:50..547"
                     /db_xref="GeneID:144112071"
ORIGIN      
        1 mpsarltfrr tesetaiiea iestessaak gwftskfkkm aayviyvlva ivilwqirgl
       61 hldassasgy ivlsiqiffa itlvlacvkl alsfrefhsq lhnedyipsk dpesgasadg
      121 gggderddfe dfcrlqgfpl pgqpksdaes ddeesgemqr velrf
//
LOCUS       XP_077501275             220 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112072 [Amblyomma americanum].
ACCESSION   XP_077501275
VERSION     XP_077501275.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645149.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526514) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..220
                     /product="uncharacterized protein LOC144112072"
                     /calculated_mol_wt=24926
     CDS             1..220
                     /gene="LOC144112072"
                     /coded_by="XM_077645149.1:31..693"
                     /db_xref="GeneID:144112072"
ORIGIN      
        1 mpfakffgrt eneaanieav vsthsttags sfnfkrkirn vmlivvavla fwknldlyse
       61 vistgismlm ptgilliliv alpwlklllr ipnwhmrlla ehihqqaitd ttaktdgsge
      121 gnldhqndhn dqddlhdfnd lhdlssqdrq ddlgdlsdle dldfqddesd qcdqgyladv
      181 ddqddkedfd nllryhglll pieesdedgf leeqdvelhf
//
LOCUS       XP_077501276             165 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112073 [Amblyomma americanum].
ACCESSION   XP_077501276
VERSION     XP_077501276.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645150.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526514) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..165
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..165
                     /product="uncharacterized protein LOC144112073"
                     /calculated_mol_wt=18244
     CDS             1..165
                     /gene="LOC144112073"
                     /coded_by="XM_077645150.1:62..559"
                     /db_xref="GeneID:144112073"
ORIGIN      
        1 mpsarltfrr tesetaiiea iestessaak gwftskfkkm aayviyvvva ivilwqirgl
       61 hldapsasgy ivlsiqiffa itlvlacvkl flgfrefhsq lhneeyipsk dpesgasadg
      121 gggderddfe dfcrlqgfpl pgqpksdaes ddeesgemqr velrf
//
LOCUS       XP_077501277             165 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112074 [Amblyomma americanum].
ACCESSION   XP_077501277
VERSION     XP_077501277.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645151.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526514) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..165
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..165
                     /product="uncharacterized protein LOC144112074"
                     /calculated_mol_wt=18224
     CDS             1..165
                     /gene="LOC144112074"
                     /coded_by="XM_077645151.1:11..508"
                     /db_xref="GeneID:144112074"
ORIGIN      
        1 mpsarltfrr tesetaiiev iestessaak gwfnskfkkm aayviyvvva ivilwqirgl
       61 hldapsasgy ivlsiqiffa itlvlacvkl alsfrefhsq lhnedyipsk dpesgasadg
      121 gggderndfe dfcrlqgfpl pgqpksdaes ddeesgemqr velrf
//
LOCUS       XP_077501278             112 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112076 [Amblyomma americanum].
ACCESSION   XP_077501278
VERSION     XP_077501278.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645152.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526519) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..112
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..112
                     /product="uncharacterized protein LOC144112076"
                     /calculated_mol_wt=11722
     CDS             1..112
                     /gene="LOC144112076"
                     /coded_by="XM_077645152.1:61..399"
                     /db_xref="GeneID:144112076"
ORIGIN      
        1 mqsligylff lvrigiesas plqstaapve vmlghdylpl psplglgtia gadsavhdtd
       61 dphssrapql tdvqttpppp adeegaaqst pirviasasw dspdygkysr kh
//
LOCUS       XP_077501280             143 aa            linear   INV 25-NOV-2025
DEFINITION  importin-7-like [Amblyomma americanum].
ACCESSION   XP_077501280
VERSION     XP_077501280.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645154.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526521) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..143
                     /product="importin-7-like"
                     /calculated_mol_wt=16099
     CDS             1..143
                     /gene="LOC144112079"
                     /coded_by="XM_077645154.1:15..446"
                     /db_xref="GeneID:144112079"
ORIGIN      
        1 mtlatrvsts qrewrgapfp vegdeseegi estmtpldee hrpveacqvs kgamrgiqnt
       61 dplwfgeltl dplsvkqprs pqevcgltgq rkaarvepdr eewrirvpep dgalvvqlwr
      121 htllratvlq prrlptpfrp srp
//
LOCUS       XP_077501281             138 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112080 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501281
VERSION     XP_077501281.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645155.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526521) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..138
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..138
                     /product="uncharacterized protein LOC144112080 isoform X1"
                     /calculated_mol_wt=15041
     CDS             1..138
                     /gene="LOC144112080"
                     /coded_by="XM_077645155.1:325..741"
                     /db_xref="GeneID:144112080"
ORIGIN      
        1 mqyftrglpl qsgqqkqlyy avpvasaqev taegivatql esscyqgnat sstmsekmht
       61 mmlgvsilti lpilstneak dasvqmtdmp wstlhgllrs lrsavlrhip qnaglskris
      121 rrfcgrgnfv srgqtfds
//
LOCUS       XP_077501282             114 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112080 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501282
VERSION     XP_077501282.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645156.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526521) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..114
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..114
                     /product="uncharacterized protein LOC144112080 isoform X2"
                     /calculated_mol_wt=12410
     CDS             1..114
                     /gene="LOC144112080"
                     /coded_by="XM_077645156.1:14..358"
                     /db_xref="GeneID:144112080"
ORIGIN      
        1 maatcswfsg hvkgkktgvs efkvekamyd tmkgipkdav fhkrtaitiw steavvlcss
       61 sgkcsggdsg rhcgytagvk llsgkcnlfd yvrkdahyda rclhldytan fvyq
//
LOCUS       XP_077501283             391 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112081 [Amblyomma americanum].
ACCESSION   XP_077501283
VERSION     XP_077501283.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645157.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526521) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..391
                     /product="uncharacterized protein LOC144112081"
                     /calculated_mol_wt=42866
     Region          <170..>367
                     /region_name="COG4372"
                     /note="Uncharacterized protein, contains DUF3084 domain
                     [Function unknown]"
                     /db_xref="CDD:443500"
     CDS             1..391
                     /gene="LOC144112081"
                     /coded_by="XM_077645157.1:2..1177"
                     /db_xref="GeneID:144112081"
ORIGIN      
        1 mqttvlafas lllpslagls tgassvcalp ytgddpgsch lppdecssll dvppeelhtp
       61 eitvpwvats tsatmptcik elhpltplrg lgdcttaqpp npltfamqvs tsyalfakks
      121 snyilvqfps phccvaiave cahvihsllm lsgdvetnpg pegnaavlae lqklnagqtl
      181 liteiqdlkt qlnttdqtia sldkrmadle ahyqtllhlr ndieimqstt inqakkieel
      241 etrlddaenq srrnnlifyg ipdpasaetw aeseklvidv crnnlditle pndierahrl
      301 gnhsadrtrp vivkflshkt kdallsngrk lkdtnysige dfsrtvrhar kqllvfakan
      361 snkyslrfkt lhigskryvf dassqtvkei a
//
LOCUS       XP_077501285             317 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112084 [Amblyomma americanum].
ACCESSION   XP_077501285
VERSION     XP_077501285.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645159.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526521) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..317
                     /product="uncharacterized protein LOC144112084"
                     /calculated_mol_wt=36008
     Region          <194..254
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     CDS             1..317
                     /gene="LOC144112084"
                     /coded_by="XM_077645159.1:1..954"
                     /db_xref="GeneID:144112084"
ORIGIN      
        1 mhdlpvnwkh elsefwsggv llafpeahqd nkffkknats fprfhvvhse heeqtarqvs
       61 pfivtrtlts aigegykikt langdlllev lynhqqekls evksfgdlpv iisthrslnt
      121 vrgvisdddl kyvtdeelle glkeqnvtnv yrikirrdne eiptkqialt fafsilpdsv
      181 eigyiklqlr eyipnprrcf kcqryghssq scrgqltcak csfhehdaen cavephlcvn
      241 cegshpaysr acpvwkqeke ivtikvkeni tfrearkrva sihkptfsdv vqrgtapqpl
      301 laptqatrrt vgdsnit
//
LOCUS       XP_077501286             198 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112088 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501286
VERSION     XP_077501286.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645160.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526522) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..198
                     /product="uncharacterized protein LOC144112088 isoform X1"
                     /calculated_mol_wt=21363
     CDS             1..198
                     /gene="LOC144112088"
                     /coded_by="XM_077645160.1:279..875"
                     /db_xref="GeneID:144112088"
ORIGIN      
        1 mrrvlvsttr ststiaaakq ekkavtgirr vfvssiinrt maatcswfsg hgkskktgas
       61 efkvekamyd tvkgipmdtv fhkrtaipiw steavvlcss sgkcsggdsg rqgkgivatq
      121 lesscyqgns tystlpekth ammlgasilt ilpilstnea kdafvqiaes qqppwkatte
      181 wlrscwgret gmvvekfg
//
LOCUS       XP_077501287             186 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112088 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501287
VERSION     XP_077501287.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645161.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526522) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..186
                     /product="uncharacterized protein LOC144112088 isoform X2"
                     /calculated_mol_wt=19966
     CDS             1..186
                     /gene="LOC144112088"
                     /coded_by="XM_077645161.1:439..999"
                     /db_xref="GeneID:144112088"
ORIGIN      
        1 msplsrfgav lvcacsayia ehniinrtma atcswfsghg kskktgasef kvekamydtv
       61 kgipmdtvfh krtaipiwst eavvlcsssg kcsggdsgrq gkgivatqle sscyqgnsty
      121 stlpektham mlgasiltil pilstneakd afvqiaesqq ppwkattewl rscwgretgm
      181 vvekfg
//
LOCUS       XP_077501288             477 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112092 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501288
VERSION     XP_077501288.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645162.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526524) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..477
                     /product="uncharacterized protein LOC144112092 isoform X1"
                     /calculated_mol_wt=51393
     Region          3..86
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..477
                     /gene="LOC144112092"
                     /coded_by="XM_077645162.1:145..1578"
                     /db_xref="GeneID:144112092"
ORIGIN      
        1 mrcvavgckt grksglrihs fprdpkrrle wavkvniaen gklwvppkdc cygvcelflq
       61 ahfeedqyep rrtdrklkpf aiptlfshrk vpkrrrmlvr gnpaenssag ttasftgaga
      121 vtptstvats qqaglcsatn sadaaawaqr aaqstvtsre paftvatsgg niilvpattl
      181 sqnqgliidq ppvavqarat tgvntitvat snkfqavnpr rsrcqhkmlr ygqlgrtdaf
      241 tsvpvpraaa aparpqnivl pplgclpnag piilvpmsqp slltavpram peqssavrsa
      301 vpgqggsppm pvilsvesls gkassvtsgv vgpppliarp ptydelrrev ttlkqqsadl
      361 rrdiceaarq aarvkaalsr qserlshflr adqverlvql pgdapvrwte ptlrfaldiy
      421 rcspkayrtm laahyplpse ralrafcveh gvqdgvppel llqaepgeed ganivwl
//
LOCUS       XP_077501289             473 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112092 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501289
VERSION     XP_077501289.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645163.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526524) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..473
                     /product="uncharacterized protein LOC144112092 isoform X2"
                     /calculated_mol_wt=50892
     Region          3..82
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..473
                     /gene="LOC144112092"
                     /coded_by="XM_077645163.1:142..1563"
                     /db_xref="GeneID:144112092"
ORIGIN      
        1 mrcvavgckt grksglrihs fprdpkrrle wavkvniaen gklwvppkdc cygvceahfe
       61 edqyeprrtd rklkpfaipt lfshrkvpkr rrmlvrgnpa enssagttas ftgagavtpt
      121 stvatsqqag lcsatnsada aawaqraaqs tvtsrepaft vatsggniil vpattlsqnq
      181 gliidqppva vqarattgvn titvatsnkf qavnprrsrc qhkmlrygql grtdaftsvp
      241 vpraaaapar pqnivlpplg clpnagpiil vpmsqpsllt avprampeqs savrsavpgq
      301 ggsppmpvil sveslsgkas svtsgvvgpp pliarpptyd elrrevttlk qqsadlrrdi
      361 ceaarqaarv kaalsrqser lshflradqv erlvqlpgda pvrwteptlr faldiyrcsp
      421 kayrtmlaah yplpseralr afcvehgvqd gvppelllqa epgeedgani vwl
//
LOCUS       XP_077501290             470 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112092 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501290
VERSION     XP_077501290.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645164.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526524) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..470
                     /product="uncharacterized protein LOC144112092 isoform X3"
                     /calculated_mol_wt=50763
     Region          3..86
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..470
                     /gene="LOC144112092"
                     /coded_by="XM_077645164.1:145..1557"
                     /db_xref="GeneID:144112092"
ORIGIN      
        1 mrcvavgckt grksglrihs fprdpkrrle wavkvniaen gklwvppkdc cygvcelflq
       61 ahfeedqyep rrtdrklkpf aiptlfshrk vpkrrrmlvr gnpaenssag ttasftgaga
      121 vtptstvats qqaglcsaaw aqraaqstvt srepaftvat sggniilvpa ttlsqnqgli
      181 idqppvavqa rattgvntit vatsnkfqav nprrsrcqhk mlrygqlgrt daftsvpvpr
      241 aaaaparpqn ivlpplgclp nagpiilvpm sqpslltavp rampeqssav rsavpgqggs
      301 ppmpvilsve slsgkassvt sgvvgpppli arpptydelr revttlkqqs adlrrdicea
      361 arqaarvkaa lsrqserlsh flradqverl vqlpgdapvr wteptlrfal diyrcspkay
      421 rtmlaahypl pseralrafc vehgvqdgvp pelllqaepg eedganivwl
//
LOCUS       XP_077501291             466 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112092 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077501291
VERSION     XP_077501291.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645165.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526524) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..466
                     /product="uncharacterized protein LOC144112092 isoform X4"
                     /calculated_mol_wt=50318
     Region          3..86
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..466
                     /gene="LOC144112092"
                     /coded_by="XM_077645165.1:145..1545"
                     /db_xref="GeneID:144112092"
ORIGIN      
        1 mrcvavgckt grksglrihs fprdpkrrle wavkvniaen gklwvppkdc cygvcelflq
       61 ahfeedqyep rrtdrklkpf aiptlfshrk vpkrrrmlvr gnpaenssag ttasftgaga
      121 vtptstvats qqaglcsatn sadaaawaqr aaqstvtsre paftvatsgg niilvpattl
      181 sqnqgliidq ppvavqarat tgvntitvat snkfqavnpr rsrcqhkmlr ygqlgrtdaf
      241 tsvpvpraaa aparpqnigp iilvpmsqps lltavpramp eqssavrsav pgqggsppmp
      301 vilsveslsg kassvtsgvv gpppliarpp tydelrrevt tlkqqsadlr rdiceaarqa
      361 arvkaalsrq serlshflra dqverlvqlp gdapvrwtep tlrfaldiyr cspkayrtml
      421 aahyplpser alrafcvehg vqdgvppell lqaepgeedg anivwl
//
LOCUS       XP_077501292             459 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112092 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077501292
VERSION     XP_077501292.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645166.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526524) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..459
                     /product="uncharacterized protein LOC144112092 isoform X5"
                     /calculated_mol_wt=49164
     Region          3..86
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..459
                     /gene="LOC144112092"
                     /coded_by="XM_077645166.1:143..1522"
                     /db_xref="GeneID:144112092"
ORIGIN      
        1 mrcvavgckt grksglrihs fprdpkrrle wavkvniaen gklwvppkdc cygvcelflq
       61 ahfeedqyep rrtdrklkpf aiptlfshrk vpkrrrmlvr gnpaenssag ttasftgaga
      121 vtptstvats qqaglcsatn sadaaawaqr aaqstvtsre paftvatsgg niilvpattl
      181 sqnqgliidq ppvavqarat tgvntitvat snkfqavnpn aftsvpvpra aaaparpqni
      241 vlpplgclpn agpiilvpms qpslltavpr ampeqssavr savpgqggsp pmpvilsves
      301 lsgkassvts gvvgppplia rpptydelrr evttlkqqsa dlrrdiceaa rqaarvkaal
      361 srqserlshf lradqverlv qlpgdapvrw teptlrfald iyrcspkayr tmlaahyplp
      421 seralrafcv ehgvqdgvpp elllqaepge edganivwl
//
LOCUS       XP_077501293             448 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112092 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077501293
VERSION     XP_077501293.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645167.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526524) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..448
                     /product="uncharacterized protein LOC144112092 isoform X6"
                     /calculated_mol_wt=48088
     Region          3..86
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          107..>258
                     /region_name="PHA03255"
                     /note="BDLF3; Provisional"
                     /db_xref="CDD:165513"
     CDS             1..448
                     /gene="LOC144112092"
                     /coded_by="XM_077645167.1:144..1490"
                     /db_xref="GeneID:144112092"
ORIGIN      
        1 mrcvavgckt grksglrihs fprdpkrrle wavkvniaen gklwvppkdc cygvcelflq
       61 ahfeedqyep rrtdrklkpf aiptlfshrk vpkrrrmlvr gnpaenssag ttasftgaga
      121 vtptstvats qqaglcsatn sadaaawaqr aaqstvtsre paftvatsgg niilvpattl
      181 sqnqgliidq ppvavqarat tgvntitvat snkfqavnpn aftsvpvpra aaaparpqni
      241 gpiilvpmsq pslltavpra mpeqssavrs avpgqggspp mpvilsvesl sgkassvtsg
      301 vvgpppliar pptydelrre vttlkqqsad lrrdiceaar qaarvkaals rqserlshfl
      361 radqverlvq lpgdapvrwt eptlrfaldi yrcspkayrt mlaahyplps eralrafcve
      421 hgvqdgvppe lllqaepgee dganivwl
//
LOCUS       XP_077501294             387 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112092 isoform X7 [Amblyomma
            americanum].
ACCESSION   XP_077501294
VERSION     XP_077501294.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645168.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526524) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..387
                     /product="uncharacterized protein LOC144112092 isoform X7"
                     /calculated_mol_wt=41023
     CDS             1..387
                     /gene="LOC144112092"
                     /coded_by="XM_077645168.1:54..1217"
                     /db_xref="GeneID:144112092"
ORIGIN      
        1 mgceseycre rqtlgasqgl llrclrgaga vtptstvats qqaglcsatn sadaaawaqr
       61 aaqstvtsre paftvatsgg niilvpattl sqnqgliidq ppvavqarat tgvntitvat
      121 snkfqavnpr rsrcqhkmlr ygqlgrtdaf tsvpvpraaa aparpqnivl pplgclpnag
      181 piilvpmsqp slltavpram peqssavrsa vpgqggsppm pvilsvesls gkassvtsgv
      241 vgpppliarp ptydelrrev ttlkqqsadl rrdiceaarq aarvkaalsr qserlshflr
      301 adqverlvql pgdapvrwte ptlrfaldiy rcspkayrtm laahyplpse ralrafcveh
      361 gvqdgvppel llqaepgeed ganivwl
//
LOCUS       XP_077501295             380 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112092 isoform X8 [Amblyomma
            americanum].
ACCESSION   XP_077501295
VERSION     XP_077501295.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645169.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526524) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..380
                     /product="uncharacterized protein LOC144112092 isoform X8"
                     /calculated_mol_wt=40392
     CDS             1..380
                     /gene="LOC144112092"
                     /coded_by="XM_077645169.1:54..1196"
                     /db_xref="GeneID:144112092"
ORIGIN      
        1 mgceseycre rqtlgasqgl llrclrgaga vtptstvats qqaglcsaaw aqraaqstvt
       61 srepaftvat sggniilvpa ttlsqnqgli idqppvavqa rattgvntit vatsnkfqav
      121 nprrsrcqhk mlrygqlgrt daftsvpvpr aaaaparpqn ivlpplgclp nagpiilvpm
      181 sqpslltavp rampeqssav rsavpgqggs ppmpvilsve slsgkassvt sgvvgpppli
      241 arpptydelr revttlkqqs adlrrdicea arqaarvkaa lsrqserlsh flradqverl
      301 vqlpgdapvr wteptlrfal diyrcspkay rtmlaahypl pseralrafc vehgvqdgvp
      361 pelllqaepg eedganivwl
//
LOCUS       XP_077501296             379 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112092 isoform X9 [Amblyomma
            americanum].
ACCESSION   XP_077501296
VERSION     XP_077501296.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645170.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526524) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..379
                     /product="uncharacterized protein LOC144112092 isoform X9"
                     /calculated_mol_wt=39949
     Region          3..86
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..379
                     /gene="LOC144112092"
                     /coded_by="XM_077645170.1:145..1284"
                     /db_xref="GeneID:144112092"
ORIGIN      
        1 mrcvavgckt grksglrihs fprdpkrrle wavkvniaen gklwvppkdc cygvcelflq
       61 ahfeedqyep rrtdrklkpf aiptlfshrk vpkrrrmlvr gnpaenssag ttasftgaga
      121 vtptstvats qqaglcsatn sadaaawaqr aaqstvtsre paftvatsgg niilvpattl
      181 sqnqgliidq ppvavqarat tgvntitvat snkfqavnpr rsrcqhkmlr ygqlgrtdaf
      241 tsvpvpraaa aparpqnivl pplgclpnag piilvpmsqp slltavpram peqssavrsa
      301 vpgqggsppm pvilsvesls gkassvtsgv vgpppliarl ashqhvnavr dtsicfprgg
      361 kpgdlpdqgl ppmtscdgr
//
LOCUS       XP_077501297             377 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112092 isoform X10 [Amblyomma
            americanum].
ACCESSION   XP_077501297
VERSION     XP_077501297.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645171.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526524) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..377
                     /product="uncharacterized protein LOC144112092 isoform
                     X10"
                     /calculated_mol_wt=40861
     Region          3..86
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..377
                     /gene="LOC144112092"
                     /coded_by="XM_077645171.1:144..1277"
                     /db_xref="GeneID:144112092"
ORIGIN      
        1 mrcvavgckt grksglrihs fprdpkrrle wavkvniaen gklwvppkdc cygvcelflq
       61 ahfeedqyep rrtdrklkpf aiptlfshrk vpkrrrmlvr gnpaenssag ttasftgaga
      121 vtptstvats qqaglcsdaf tsvpvpraaa aparpqnivl pplgclpnag piilvpmsqp
      181 slltavpram peqssavrsa vpgqggsppm pvilsvesls gkassvtsgv vgpppliarp
      241 ptydelrrev ttlkqqsadl rrdiceaarq aarvkaalsr qserlshflr adqverlvql
      301 pgdapvrwte ptlrfaldiy rcspkayrtm laahyplpse ralrafcveh gvqdgvppel
      361 llqaepgeed ganivwl
//
LOCUS       XP_077501298             366 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112092 isoform X11 [Amblyomma
            americanum].
ACCESSION   XP_077501298
VERSION     XP_077501298.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645172.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526524) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..366
                     /product="uncharacterized protein LOC144112092 isoform
                     X11"
                     /calculated_mol_wt=39786
     Region          3..86
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..366
                     /gene="LOC144112092"
                     /coded_by="XM_077645172.1:145..1245"
                     /db_xref="GeneID:144112092"
ORIGIN      
        1 mrcvavgckt grksglrihs fprdpkrrle wavkvniaen gklwvppkdc cygvcelflq
       61 ahfeedqyep rrtdrklkpf aiptlfshrk vpkrrrmlvr gnpaenssag ttasftgaga
      121 vtptstvats qqaglcsdaf tsvpvpraaa aparpqnigp iilvpmsqps lltavpramp
      181 eqssavrsav pgqggsppmp vilsveslsg kassvtsgvv gpppliarpp tydelrrevt
      241 tlkqqsadlr rdiceaarqa arvkaalsrq serlshflra dqverlvqlp gdapvrwtep
      301 tlrfaldiyr cspkayrtml aahyplpser alrafcvehg vqdgvppell lqaepgeedg
      361 anivwl
//
LOCUS       XP_077501299             310 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112095 [Amblyomma americanum].
ACCESSION   XP_077501299
VERSION     XP_077501299.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645173.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526524) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 16% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..310
                     /product="uncharacterized protein LOC144112095"
                     /calculated_mol_wt=35513
     CDS             1..310
                     /gene="LOC144112095"
                     /coded_by="XM_077645173.1:1..933"
                     /db_xref="GeneID:144112095"
ORIGIN      
        1 mwyscewaec fdivnqllal sgdvelnpgp ntrhqvqdsl pdvqskqled kfailealnt
       61 rpakiqldhk afiksiddlk ksqlkledls emnkrltnve rktvtidete kevhpfqqlv
      121 dqlssensqr rarlseledr qrrdnllfyg mpdaqseswa qseeklvkvl sscleipsse
      181 ilidrahrlg gfasekcrpi iakfssfkvk qrillncskl kdekitlsed fsfatrhark
      241 klvefgksqp ltfklrfnkl yiknkcysyn hsddyvheip safplptdrs adptndthsa
      301 sapptelvtn
//
LOCUS       XP_077501301             266 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112098 [Amblyomma americanum].
ACCESSION   XP_077501301
VERSION     XP_077501301.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645175.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526530) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..266
                     /product="uncharacterized protein LOC144112098"
                     /calculated_mol_wt=29342
     CDS             1..266
                     /gene="LOC144112098"
                     /coded_by="XM_077645175.1:1..801"
                     /db_xref="GeneID:144112098"
ORIGIN      
        1 mtgsvgaasv amsgrgnrft deenayqvvl pglptgrfvv ntvflhadir arpyrvedfr
       61 dalaqharlp diiamgayrm shvwavtlkd seavtkilsv cemlvkesrc lvidptnqdv
      121 rlklhwllhn vpdedvraaf apygkvteia rerwrvrgvp ekgsttrlvt lklktgvkfe
      181 dlphqlnvsg elalvvvpgr pplclrcrst dhirkecrvp rcgacrrfgh edgqcsrtya
      241 siagpgtsed saellvdead aedaar
//
LOCUS       XP_077501316             385 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112334 [Amblyomma americanum].
ACCESSION   XP_077501316
VERSION     XP_077501316.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645190.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526537) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..385
                     /product="uncharacterized protein LOC144112334"
                     /calculated_mol_wt=42987
     Region          308..>382
                     /region_name="MULE"
                     /note="MULE transposase domain; pfam10551"
                     /db_xref="CDD:463145"
     CDS             1..385
                     /gene="LOC144112334"
                     /coded_by="XM_077645190.1:253..1410"
                     /db_xref="GeneID:144112334"
ORIGIN      
        1 megpsappae spvrdkyqcp vcqaefvleq srrrhvrkqh peaasslsgs akasyscdkc
       61 tnvlarrgql lqhheqnhgf vsrlsthqfs sysdfaawke meemkekvkf iassgpkhss
      121 sgvmqmcllc nrsgmhvkag egkrqpklvg svkcnkhcfa tmavtqegen vtveyqaery
      181 ghdksvkhqr ltstqknaia aklslgvpsk rilldahatg gdelnvlhtl nrhdvynvkr
      241 kfhigyeeqk dpdefvsmrt widelqetsa llyankellt eqgigkfkma lmnstqkefl
      301 leygkksvsi dstygtnshg lqlttllvig kdwsgipcay llsrstteet mvaffrqskt
      361 dlvrhsalss scqmvrqhit mlgqg
//
LOCUS       XP_077501318             137 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112335 [Amblyomma americanum].
ACCESSION   XP_077501318
VERSION     XP_077501318.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645192.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526537) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..137
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..137
                     /product="uncharacterized protein LOC144112335"
                     /calculated_mol_wt=15043
     Region          <19..76
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..137
                     /gene="LOC144112335"
                     /coded_by="XM_077645192.1:206..619"
                     /db_xref="GeneID:144112335"
ORIGIN      
        1 msastawvvq mrkstetaka ispsmcryqk ahiktrnvve rtfgvwkrrf pcldmglqhk
       61 pkqaamiita caalqniacl mkepqppies apiqdappar tsttaarqpe hlppvdavsd
      121 slsgmqarwv liqksft
//
LOCUS       XP_077501320             297 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112337 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501320
VERSION     XP_077501320.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645194.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526537) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..297
                     /product="uncharacterized protein LOC144112337 isoform X1"
                     /calculated_mol_wt=34119
     Region          175..249
                     /region_name="DD"
                     /note="Death Domain Superfamily of protein-protein
                     interaction domains; cl14633"
                     /db_xref="CDD:472698"
     CDS             1..297
                     /gene="LOC144112337"
                     /coded_by="XM_077645194.1:155..1048"
                     /db_xref="GeneID:144112337"
ORIGIN      
        1 mgsskvprrl hpsqeqrchr rhtsasvgie gddqddvrfk ellrnlrmlw sherflekqe
       61 vvwrllkrdv prrarfscnt vddllnilvd reviraqevv flgrlaavfl vkeasehiqa
      121 yeeipldnkn cilcavprql pllchpssqe nnsrkelvca ilylceykcp wknvvrafpk
      181 nlsikeaeid rleamkcasq parkrcptca gfevleswrk khpqhatvkl llqylaserv
      241 nrsfaddies gfhmsmtvgl kclllksgye sqaergrsvp vqlvsaqils rdnsvnf
//
LOCUS       XP_077501321             267 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112337 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501321
VERSION     XP_077501321.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645195.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526537) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..267
                     /product="uncharacterized protein LOC144112337 isoform X2"
                     /calculated_mol_wt=30783
     Region          145..219
                     /region_name="DD"
                     /note="Death Domain Superfamily of protein-protein
                     interaction domains; cl14633"
                     /db_xref="CDD:472698"
     CDS             1..267
                     /gene="LOC144112337"
                     /coded_by="XM_077645195.1:163..966"
                     /db_xref="GeneID:144112337"
ORIGIN      
        1 mddqddvrfk ellrnlrmlw sherflekqe vvwrllkrdv prrarfscnt vddllnilvd
       61 reviraqevv flgrlaavfl vkeasehiqa yeeipldnkn cilcavprql pllchpssqe
      121 nnsrkelvca ilylceykcp wknvvrafpk nlsikeaeid rleamkcasq parkrcptca
      181 gfevleswrk khpqhatvkl llqylaserv nrsfaddies gfhmsmtvgl kclllksgye
      241 sqaergrsvp vqlvsaqils rdnsvnf
//
LOCUS       XP_077501322             250 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112337 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501322
VERSION     XP_077501322.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645196.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526537) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..250
                     /product="uncharacterized protein LOC144112337 isoform X3"
                     /calculated_mol_wt=28637
     Region          128..202
                     /region_name="DD"
                     /note="Death Domain Superfamily of protein-protein
                     interaction domains; cl14633"
                     /db_xref="CDD:472698"
     CDS             1..250
                     /gene="LOC144112337"
                     /coded_by="XM_077645196.1:302..1054"
                     /db_xref="GeneID:144112337"
ORIGIN      
        1 mlwsherfle kqevvwrllk rdvprrarfs cntvddllni lvdreviraq evvflgrlaa
       61 vflvkeaseh iqayeeipld nkncilcavp rqlpllchps sqennsrkel vcailylcey
      121 kcpwknvvra fpknlsikea eidrleamkc asqparkrcp tcagfevles wrkkhpqhat
      181 vklllqylas ervnrsfadd iesgfhmsmt vglkclllks gyesqaergr svpvqlvsaq
      241 ilsrdnsvnf
//
LOCUS       XP_077501323             237 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112338 [Amblyomma americanum].
ACCESSION   XP_077501323
VERSION     XP_077501323.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645197.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526537) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..237
                     /product="uncharacterized protein LOC144112338"
                     /calculated_mol_wt=26529
     Region          <22..70
                     /region_name="REC"
                     /note="phosphoacceptor receiver (REC) domain of response
                     regulators (RRs) and pseudo response regulators (PRRs);
                     cl19078"
                     /db_xref="CDD:473134"
     Site            order(49..52,54)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:381085"
     CDS             1..237
                     /gene="LOC144112338"
                     /coded_by="XM_077645197.1:724..1437"
                     /db_xref="GeneID:144112338"
ORIGIN      
        1 maatvltaqk nsaprlrftf addlallrev naqnpfkdaa egknilrnvn facgkpftar
       61 alrerldlll gqfiannrtn lrksgteeey eekekllqei sdlarefryk cksrkvqaaa
      121 nktfatgvrd nacaaaarvs phhndsypip sgtatdtpet asyvlqqmcn drdvvddyft
      181 tdaenfqsaq qtasanvttd vqeeteehep qptaemkrrk rkpktagdvn heflekr
//
LOCUS       XP_077501324             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112339 [Amblyomma americanum].
ACCESSION   XP_077501324
VERSION     XP_077501324.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645198.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526537) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144112339"
                     /calculated_mol_wt=22715
     CDS             1..217
                     /gene="LOC144112339"
                     /coded_by="XM_077645198.1:67..720"
                     /db_xref="GeneID:144112339"
ORIGIN      
        1 mlgqpkqksk eedarkkrdr hktgggsaqc tvsaqseqvi avashimtrv gnqtdsdggi
       61 dlppvaslpv irllqpmvdg agneefhyae ddwepplvde pqspaaavgk sgatpeaieq
      121 esnlffpavq padasstaaa gcssatvasg daagpttaea vatgdagrap rgrmallers
      181 ladeddyraa llreehglrl qlmredhnsi lqergpp
//
LOCUS       XP_077501325             116 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112340 [Amblyomma americanum].
ACCESSION   XP_077501325
VERSION     XP_077501325.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645199.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526537) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..116
                     /product="uncharacterized protein LOC144112340"
                     /calculated_mol_wt=12667
     CDS             1..116
                     /gene="LOC144112340"
                     /coded_by="XM_077645199.1:214..564"
                     /db_xref="GeneID:144112340"
ORIGIN      
        1 mpcvgilnpd rasanggvyp qilicsypsk snfqhrflda mkapkrhlfa adgyhlsage
       61 gieelaevfv rglakfygaa ilapfsdapa ryiihqchqc gskghrhgdc yeycrp
//
LOCUS       XP_077501326             148 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112341 [Amblyomma americanum].
ACCESSION   XP_077501326
VERSION     XP_077501326.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645200.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526537) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="uncharacterized protein LOC144112341"
                     /calculated_mol_wt=16759
     CDS             1..148
                     /gene="LOC144112341"
                     /coded_by="XM_077645200.1:78..524"
                     /db_xref="GeneID:144112341"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedrrr rhlnrrltat mkrlsgvhvl
       61 nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar pgevyvvhrc
      121 rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077501327             296 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112344 [Amblyomma americanum].
ACCESSION   XP_077501327
VERSION     XP_077501327.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645201.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526537) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 39% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..296
                     /product="uncharacterized protein LOC144112344"
                     /calculated_mol_wt=33034
     CDS             1..296
                     /gene="LOC144112344"
                     /coded_by="XM_077645201.1:33..923"
                     /db_xref="GeneID:144112344"
ORIGIN      
        1 megpsappae spvrdkyqcp vcqaefvleq srkrhvrkqh peaasslsgs akasyscdqc
       61 tnvlarrgql lqhheqnhgf vsrlsthqfs sysdfaawke meemkekvnf iassvpkssf
      121 sgvmqmyllc nrsgmhvktg egkrqpklvg svkcnkhcfa tmavtqegen vtveyqaehy
      181 ghdksrlqck rldkllitlr qftnerllkq lvsedvhaiq sapvpiswag sggnrsdhgk
      241 qlrdvlpwfy gelgsapeyt advvrkfnia knmdgalrlg ppivlhlqkp gkqqmp
//
LOCUS       XP_077501328             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112345 [Amblyomma americanum].
ACCESSION   XP_077501328
VERSION     XP_077501328.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645202.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526540) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144112345"
                     /calculated_mol_wt=20970
     CDS             1..181
                     /gene="LOC144112345"
                     /coded_by="XM_077645202.1:1..546"
                     /db_xref="GeneID:144112345"
ORIGIN      
        1 mrqvkenqsl lsqqfdkvne kmleiesnll nmrkkeekle alvhdlgvti rsqqqkivem
       61 edrgrrnnll vfgisetdse tsvelkkkvl ddvfherlgv rvntverihr lgkrgvdkvr
      121 pvivrffdyn ekmnlfrnsk klkgsntsls hdysqatlhk rkklwasaqh ekdngadsar
      181 a
//
LOCUS       XP_077501330             298 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112352 [Amblyomma americanum].
ACCESSION   XP_077501330
VERSION     XP_077501330.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645204.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526541) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 14% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..298
                     /product="uncharacterized protein LOC144112352"
                     /calculated_mol_wt=33570
     Region          <223..258
                     /region_name="Dimer_Tnp_hAT"
                     /note="hAT family C-terminal dimerization region;
                     pfam05699"
                     /db_xref="CDD:399013"
     CDS             1..298
                     /gene="LOC144112352"
                     /coded_by="XM_077645204.1:1..897"
                     /db_xref="GeneID:144112352"
ORIGIN      
        1 magrftgvqk ritdsgprsi fvhcsnhcld lalqevarnc evvgdalniv kdvshailes
       61 knrktvysnv vlfpgndane qdcsrpntll pfrrerfnsq tslrslqrlt ashyieksff
      121 aaidritsei rrrfeqpgme qlislerilt daaggrnfaa hdlnerlgah aadfdtsrls
      181 aqlvllptll rdqgpaaser ilqilqgksa dmremmdqvv rylqlvfsvp asaasgersf
      241 salrrvktfl rnrmtqrrlt hllllhvhkr raadlqldvi mkefvsrtae rtatfgll
//
LOCUS       XP_077501332             163 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112356 [Amblyomma americanum].
ACCESSION   XP_077501332
VERSION     XP_077501332.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645206.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526544) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..163
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..163
                     /product="uncharacterized protein LOC144112356"
                     /calculated_mol_wt=17669
     CDS             1..163
                     /gene="LOC144112356"
                     /coded_by="XM_077645206.1:40..531"
                     /db_xref="GeneID:144112356"
ORIGIN      
        1 mypalslvll yaiatnraar gldcfkcvsl dhenpacedp fhnnytadll espcmssrkn
       61 rnglfpatac iklngvyeet gqtmvvrsca ldsgtltvdt eivrmshcgg fyfedryvrg
      121 clqscfedac naasrtprsh pllllllpla pflaagtvlp lac
//
LOCUS       XP_077501333             177 aa            linear   INV 25-NOV-2025
DEFINITION  deoxycytidylate deaminase-like [Amblyomma americanum].
ACCESSION   XP_077501333
VERSION     XP_077501333.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645207.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526544) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..177
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..177
                     /product="deoxycytidylate deaminase-like"
                     /calculated_mol_wt=20133
     Region          37..164
                     /region_name="ComEB"
                     /note="Deoxycytidylate deaminase [Nucleotide transport and
                     metabolism]; COG2131"
                     /db_xref="CDD:441734"
     CDS             1..177
                     /gene="LOC144112358"
                     /coded_by="XM_077645207.1:60..593"
                     /db_xref="GeneID:144112358"
ORIGIN      
        1 mdstveeekr raaeelsdca arflkisepn crqrctkrsd ylswqdyfms saylaamrsk
       61 dpntqvvfir mitppspgyq qllivchadm naifnrngfe lrgctlytth fpcnecakmv
      121 vqsgirqvvf ldnkhpdepi yaasgliltk agvtfrrhip etkkivndfe qlaafki
//
LOCUS       XP_077501334             296 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112360 [Amblyomma americanum].
ACCESSION   XP_077501334
VERSION     XP_077501334.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645208.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526544) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..296
                     /product="uncharacterized protein LOC144112360"
                     /calculated_mol_wt=34110
     CDS             1..296
                     /gene="LOC144112360"
                     /coded_by="XM_077645208.1:66..956"
                     /db_xref="GeneID:144112360"
ORIGIN      
        1 mstdvplfvq vggrkyccrs nclflivlpc prtllvcfsd cisivglllk lsgdveenpg
       61 pdtmkiiqqv iasqteilsk lseiqenqts searildmqs rlsaieqklq tfdesrdrls
      121 kletfaerce iemttvsrkl delenrsrrn nlivrgvkee eseseedllq kvnndifdki
      181 lkhrvdtiqr ihrlgkrepg rdrpiiiklt dfrdkmkims ncfnlkgtqf svnedfskrv
      241 veirknlwns canerkngvk aklifdklkv kntlyawnee tkqrfkcqva tstsne
//
LOCUS       XP_077501335             186 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112363 [Amblyomma americanum].
ACCESSION   XP_077501335
VERSION     XP_077501335.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645209.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526545) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..186
                     /product="uncharacterized protein LOC144112363"
                     /calculated_mol_wt=19971
     CDS             1..186
                     /gene="LOC144112363"
                     /coded_by="XM_077645209.1:333..893"
                     /db_xref="GeneID:144112363"
ORIGIN      
        1 msplsrfaav lvcacsayia ehniinrtma atcswfsgqg kskktgasef kvekamydtv
       61 kgipiyavfh krtaipiwst eavvlcsssg kcsggdsgrq gkgivatqle sscyqgnsty
      121 stlpektham mlgasiltil pilstneakd afvqiaesqq ppwkattewl rscwgretgm
      181 vvekfg
//
LOCUS       XP_077501337             166 aa            linear   INV 25-NOV-2025
DEFINITION  histone-lysine N-methyltransferase set-17-like [Amblyomma
            americanum].
ACCESSION   XP_077501337
VERSION     XP_077501337.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645211.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526545) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 25% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..166
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..166
                     /product="histone-lysine N-methyltransferase set-17-like"
                     /calculated_mol_wt=18474
     Region          37..155
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(53..54,108..113,124..126,147..150)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Site            order(53..54,109..113,149)
                     /site_type="other"
                     /note="SAM binding site [polypeptide binding]"
                     /db_xref="CDD:380914"
     CDS             1..166
                     /gene="LOC144112364"
                     /coded_by="XM_077645211.1:1..501"
                     /db_xref="GeneID:144112364"
ORIGIN      
        1 mglnvqrdpq faverlvnaa elytqvrsqd aslvgeslpe lltlgkstvn gcpvgvftlk
       61 alpkglhfgp yqgvkvddie nggctwpvrr cgeffvvdgr pqqrnhwmnd vnyspskrrh
      121 nlvalfvngd iyyrtlknvg pgeemlvrys tsfakvcwgt qenqgr
//
LOCUS       XP_077501338             289 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112365 [Amblyomma americanum].
ACCESSION   XP_077501338
VERSION     XP_077501338.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645212.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526545) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 22% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..289
                     /product="uncharacterized protein LOC144112365"
                     /calculated_mol_wt=31298
     Region          155..275
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(171..172,226..231,242..244,265..268)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Site            order(171..172,227..231,267)
                     /site_type="other"
                     /note="SAM binding site [polypeptide binding]"
                     /db_xref="CDD:380914"
     CDS             1..289
                     /gene="LOC144112365"
                     /coded_by="XM_077645212.1:59..928"
                     /db_xref="GeneID:144112365"
ORIGIN      
        1 mreaieafge thresppsts pdalpdhwea pgsggpnlpe eppsreeqhe dnpidgsgtf
       61 asvytalnea ekeasdafgd thresppsts pdalpdhwea pgsgdqhlle epssreeqhd
      121 knpcdgrgmv asadtaladv erevrsqdas lvgeslpell tlgkstvngc pagvftlkal
      181 pkglhfgpyh gvkvdrieng gctwqvrrcg effvvdgrph qrnhwmndvn ytpskrrqnl
      241 vafladgdiy yhtlrnvspd eellvgysts ftksllgspr gpvalqggl
//
LOCUS       XP_077501339             465 aa            linear   INV 25-NOV-2025
DEFINITION  organic cation transporter protein-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501339
VERSION     XP_077501339.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645213.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526545) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..465
                     /product="organic cation transporter protein-like isoform
                     X1"
                     /calculated_mol_wt=52251
     Region          <1..392
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..465
                     /gene="LOC144112366"
                     /coded_by="XM_077645213.1:51..1448"
                     /db_xref="GeneID:144112366"
ORIGIN      
        1 magalvgvll fgemsdrlgr rptfftcvlv ilvfgslssl apgigwynag rfvvamgvag
       61 sqattvslrk wppflcpsar dgfgkvmeim ppqyrmmmnv gfgigytipl lllpllayyi
      121 rdwgylqlat glsalmmvpf wfilqesprw lltkgrmkra ersvvrilri nrrpvpdmdq
      181 vmsdlvlfak seskkttafh fvdffrtpkl rrntlvlfal wfmgsfvyyn lvlsstsapg
      241 npyfnyaisa aseipaallg lwvarrcsrr rsqalfllla alaivplpfl pkdspgwlri
      301 gsnvlvrflt lnagfikwim alevfptsar sfgfacvltc srfgamvapf lrdlgeatdp
      361 svpyviqalf lllasgisll lpetlnkplp dtfheaesis silqtlngsw eerqksikei
      421 qqitsqnkyk ywhrirnrhs atvnkifckr rrcslvdqsg vhyiv
//
LOCUS       XP_077501340             448 aa            linear   INV 25-NOV-2025
DEFINITION  organic cation transporter protein-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501340
VERSION     XP_077501340.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645214.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526545) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..448
                     /product="organic cation transporter protein-like isoform
                     X2"
                     /calculated_mol_wt=50321
     Region          <1..375
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..448
                     /gene="LOC144112366"
                     /coded_by="XM_077645214.1:51..1397"
                     /db_xref="GeneID:144112366"
ORIGIN      
        1 magalvgvll fgemsdrlgr rptfftcvlv ilvfgslssl apgigwynag rfvvamgvag
       61 sqattvsllm eimppqyrmm mnvgfgigyt ipllllplla yyirdwgylq latglsalmm
      121 vpfwfilqes prwlltkgrm kraersvvri lrinrrpvpd mdqvmsdlvl fakseskktt
      181 afhfvdffrt pklrrntlvl falwfmgsfv yynlvlssts apgnpyfnya isaaseipaa
      241 llglwvarrc srrrsqalfl llaalaivpl pflpkdspgw lrigsnvlvr fltlnagfik
      301 wimalevfpt sarsfgfacv ltcsrfgamv apflrdlgea tdpsvpyviq alflllasgi
      361 slllpetlnk plpdtfheae sissilqtln gsweerqksi keiqqitsqn kykywhrirn
      421 rhsatvnkif ckrrrcslvd qsgvhyiv
//
LOCUS       XP_077501341             421 aa            linear   INV 25-NOV-2025
DEFINITION  organic cation transporter protein-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501341
VERSION     XP_077501341.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645215.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526545) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..421
                     /product="organic cation transporter protein-like isoform
                     X3"
                     /calculated_mol_wt=46752
     Region          <1..392
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..421
                     /gene="LOC144112366"
                     /coded_by="XM_077645215.1:51..1316"
                     /db_xref="GeneID:144112366"
ORIGIN      
        1 magalvgvll fgemsdrlgr rptfftcvlv ilvfgslssl apgigwynag rfvvamgvag
       61 sqattvslrk wppflcpsar dgfgkvmeim ppqyrmmmnv gfgigytipl lllpllayyi
      121 rdwgylqlat glsalmmvpf wfilqesprw lltkgrmkra ersvvrilri nrrpvpdmdq
      181 vmsdlvlfak seskkttafh fvdffrtpkl rrntlvlfal wfmgsfvyyn lvlsstsapg
      241 npyfnyaisa aseipaallg lwvarrcsrr rsqalfllla alaivplpfl pkdspgwlri
      301 gsnvlvrflt lnagfikwim alevfptsar sfgfacvltc srfgamvapf lrdlgeatdp
      361 svpyviqalf lllasgisll lpetlnkplp dtfheaesis rkkipkppkq laeykianaa
      421 p
//
LOCUS       XP_077501342             367 aa            linear   INV 25-NOV-2025
DEFINITION  organic cation transporter protein-like isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077501342
VERSION     XP_077501342.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645216.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526545) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..367
                     /product="organic cation transporter protein-like isoform
                     X4"
                     /calculated_mol_wt=40861
     Region          <1..351
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..367
                     /gene="LOC144112366"
                     /coded_by="XM_077645216.1:51..1154"
                     /db_xref="GeneID:144112366"
ORIGIN      
        1 magalvgvll fgemsdrlgr rptfftcvlv ilvfgslssl apgigwynag rfvvamgvag
       61 sqattvslrk wppflcpsar dgfgkvmeim ppqyrmmmnv gfgigytipl lllpllayyi
      121 rdwgylqlat glsalmmvpf wfilqesprw lltkgrmkra ersvvrilri nrrpvpdmdq
      181 vmsdlvlfak seskkttafh fvdffrtpkl rrntlvlfal wfmgsfvyyn lvlsstsapg
      241 npyfnyaisa aseipaallg lwvarrcsrr rsqalfllla alaivplpfl pkdspgwlri
      301 gsnvlvrflt lnagfikwim alevfptsar sfgfacvltc srfgamvapf lrdleedsqa
      361 tqaagrv
//
LOCUS       XP_077501343             167 aa            linear   INV 25-NOV-2025
DEFINITION  histone-lysine N-methyltransferase set-17-like [Amblyomma
            americanum].
ACCESSION   XP_077501343
VERSION     XP_077501343.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645217.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526545) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 55% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..167
                     /product="histone-lysine N-methyltransferase set-17-like"
                     /calculated_mol_wt=18271
     Region          34..151
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(47..48,102..107,118..120,141..144)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Site            order(47..48,103..107,143)
                     /site_type="other"
                     /note="SAM binding site [polypeptide binding]"
                     /db_xref="CDD:380914"
     CDS             1..167
                     /gene="LOC144112367"
                     /coded_by="XM_077645217.1:1..504"
                     /db_xref="GeneID:144112367"
ORIGIN      
        1 mvagadstsd taladvksem isedaslvsk nifeflalgk stvkgvpmgv ftlkalpkgl
       61 hfgpyhgvkv dcieyggctw pvrrcaeffv vdgrqqeryh wmnnvnysps krrqnlvavl
      121 fngniyyrtl knvgpgeell vgystsfaes llgnprgrga lkegecr
//
LOCUS       XP_077501344             142 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger and BTB domain-containing protein 41-like [Amblyomma
            americanum].
ACCESSION   XP_077501344
VERSION     XP_077501344.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645218.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526545) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..142
                     /product="zinc finger and BTB domain-containing protein
                     41-like"
                     /calculated_mol_wt=16101
     Region          2..22
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          2..22
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          14..39
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          30..51
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..142
                     /gene="LOC144112368"
                     /coded_by="XM_077645218.1:212..640"
                     /db_xref="GeneID:144112368"
ORIGIN      
        1 mchvckkrfs kpdhlkvhmm ihsgekpyec gqcgkrfrqr vharrheevi hfrrypltcq
       61 rcgagaedvf klrhhtckpl gttedkeglk rcrgrprlra dgtspkvrpq nttegtplqg
      121 gvkrgrgrpr kavtqdasap ra
//
LOCUS       XP_077501345             214 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112369, partial [Amblyomma
            americanum].
ACCESSION   XP_077501345
VERSION     XP_077501345.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645219.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526546) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on the carboxy end.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..>214
                     /product="uncharacterized protein LOC144112369"
     CDS             1..214
                     /gene="LOC144112369"
                     /coded_by="XM_077645219.1:71..>713"
                     /db_xref="GeneID:144112369"
ORIGIN      
        1 mptlhsgkhv dyhhlnsacn sdvestpqdt vshnaaldca gssswnldsa phhhadehap
       61 dgipiirtrt pmgasgflss qhalgvggag qegispeqqr fnhhivklll eirsdikelk
      121 gnysatldme tgsdavmvat slekladiee vladkdqral qirklaligg ettdettkli
      181 mktlvktpva lrytlegrge kqsfknlrly saiv
//
LOCUS       XP_077501346             238 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112370, partial [Amblyomma
            americanum].
ACCESSION   XP_077501346
VERSION     XP_077501346.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645220.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526547) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: incomplete on the carboxy end.
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..>238
                     /product="uncharacterized protein LOC144112370"
     Region          9..73
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..238
                     /gene="LOC144112370"
                     /coded_by="XM_077645220.1:112..>825"
                     /db_xref="GeneID:144112370"
ORIGIN      
        1 maprrlnhcf apgcktgcvh vkgapkpslf svpkdealrr lweknlhrad kpledtsavc
       61 elhfepryvi rdyvhiiggk evriprgkpr gkrgvvycts vltsrnevcs ekvvmffqsq
      121 thsyykvfvr gillkestlq tsgeaedvlr atdsmsvctg vmrledyeer yfmknlkshv
      181 vtleqmyfss nclgqvaerg tqcvscrylk ralqirrarv krtdkksgrs vvlkcdlq
//
LOCUS       XP_077501347             255 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112371 [Amblyomma americanum].
ACCESSION   XP_077501347
VERSION     XP_077501347.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645221.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526547) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..255
                     /product="uncharacterized protein LOC144112371"
                     /calculated_mol_wt=28365
     CDS             1..255
                     /gene="LOC144112371"
                     /coded_by="XM_077645221.1:44..811"
                     /db_xref="GeneID:144112371"
ORIGIN      
        1 mrgknaaiqa ealeaqiskl pprqqeavrq cfaaskvkpn glrytkmwvl dciimkmksp
       61 rlyehlrrns ilslpckstl kcyvsayrtv fgfsdkvlrq lkvksaelda wkkhgglvkl
      121 sehfavktsg viegfvdlgp ftaakdkgvp cdhgmvilfv pfqgkwsqvi gyfathgnmk
      181 gdilakvvie atvlaeksgl fvdfitcdga swnrrmwril giqasaestt ckiqhpsdas
      241 rnlffisdfp hliks
//
LOCUS       XP_077501348             121 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112372 [Amblyomma americanum].
ACCESSION   XP_077501348
VERSION     XP_077501348.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645222.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526547) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..121
                     /product="uncharacterized protein LOC144112372"
                     /calculated_mol_wt=13321
     CDS             1..121
                     /gene="LOC144112372"
                     /coded_by="XM_077645222.1:20..385"
                     /db_xref="GeneID:144112372"
ORIGIN      
        1 mvntavgfrl ltepgaiddv rgtielftwk dseppapaev ldigkarvle tkrarlasag
       61 lrdegdvmte slecspneef csqcenmrre ldvvkrrned leaahgmsdh hgavkgcynl
      121 y
//
LOCUS       XP_077501349             120 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112373 [Amblyomma americanum].
ACCESSION   XP_077501349
VERSION     XP_077501349.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645223.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526547) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 14% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..120
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..120
                     /product="uncharacterized protein LOC144112373"
                     /calculated_mol_wt=12129
     CDS             1..120
                     /gene="LOC144112373"
                     /coded_by="XM_077645223.1:195..557"
                     /db_xref="GeneID:144112373"
ORIGIN      
        1 mgvrlanpfd sdrhqadpgg esqptpsvaa llsrtqdgsi pgasgdaedp lwswhyeegs
       61 pqqdeqmeee pvamaaslts attataapsv lpaastaraa gtsrmssqrq saavppapsg
//
LOCUS       XP_077501350             337 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112374, partial [Amblyomma
            americanum].
ACCESSION   XP_077501350
VERSION     XP_077501350.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645224.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526547) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         <1..337
                     /product="uncharacterized protein LOC144112374"
     Region          86..132
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          167..327
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..337
                     /gene="LOC144112374"
                     /coded_by="XM_077645224.1:<1..1014"
                     /db_xref="GeneID:144112374"
ORIGIN      
        1 fsvflcsarg neartqvnqk ivcdaevqck pavvshqsgr dsrtacftgy dsvqacaaam
       61 kdlcgvspdi yalllsimpr saertcdvpi tdrlvlflmk lklgityssl avlfcvsrnt
      121 vsrhfksvlk tlsiatqkwi yrppdhvirq tipncfkvhy qdcsmiidcr evrteqpytv
      181 qqqrvlyshy kggytlkflv gitpcgavcf gskayggrls dsfitvdsgf ldlvqpgdvv
      241 madkgfpgik agleevravl vmppflqgqd qfsesevhet yqiaqvrihv eriiqcikmf
      301 nilntripte liavmsevfh vccalanlqp piisseq
//
LOCUS       XP_077501351             358 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112375 [Amblyomma americanum].
ACCESSION   XP_077501351
VERSION     XP_077501351.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645225.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526547) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..358
                     /product="uncharacterized protein LOC144112375"
                     /calculated_mol_wt=39855
     Region          96..141
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          176..343
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..358
                     /gene="LOC144112375"
                     /coded_by="XM_077645225.1:103..1179"
                     /db_xref="GeneID:144112375"
ORIGIN      
        1 metetdlsmq nishlieeng rlkqrlasle eecsaakqka gelenkhtnh aiitkevvmq
       61 saktvkfytg fvstamftaf lqfvlsawkp scatwldaet qlimvlmrlr lglltqdlsc
      121 rfgisnatvs sifhawldvl aanmtkliiw psrqtlqanr paafqdpffd gvtgiidcte
      181 vfiqrpilmt arsqtyshyk hhntvkilvv ispsgaitfv skawggrtpd keitlrsgll
      241 dkvkegdvfl vdrgfrcgem faargathlm psltknraql pgaevttsrk vssvrihver
      301 aiqrlkvfri lqtvlplnfv krtgdknlat idkvvvvcsa lvnlhtpiik nsqrrsas
//
LOCUS       XP_077501352             605 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112376 [Amblyomma americanum].
ACCESSION   XP_077501352
VERSION     XP_077501352.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645226.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526547) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 23% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..605
                     /product="uncharacterized protein LOC144112376"
                     /calculated_mol_wt=65053
     Region          114..585
                     /region_name="AFD_class_I"
                     /note="Adenylate forming domain, Class I superfamily;
                     cl17068"
                     /db_xref="CDD:473059"
     Site            order(246,249..254,256..257)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341237"
     Site            order(249,365..366,388..393,471,483,486,497,585)
                     /site_type="other"
                     /note="AMP binding site [chemical binding]"
                     /db_xref="CDD:341237"
     Site            order(249,291..292,338,340..341,344,365..366,388..393,471,
                     483,486,494..497,566)
                     /site_type="active"
                     /db_xref="CDD:341237"
     Site            order(291,340..341,344,365,494..496,559,566)
                     /site_type="other"
                     /note="CoA binding site [chemical binding]"
                     /db_xref="CDD:341237"
     CDS             1..605
                     /gene="LOC144112376"
                     /coded_by="XM_077645226.1:1..1818"
                     /db_xref="GeneID:144112376"
ORIGIN      
        1 mtenqpdqal pnlecvsaaq agdntpeapa wtkadilgaq gyttralgpr qrpgppggei
       61 yapsccpnsf hsrpkpmrlp nlppkeptae aalvtgmgvv pvplyldlps yaipqvdaks
      121 gsrytfgell dtcrrvaagl rrlglqpgnv vafkaatsse llvamcgtlf aggitalika
      181 slteartcka cltltlqlss ytlrgsavii gitgasggat slsqlkqspl ddcesrnlcp
      241 dsvlaiiyss gttgspkgvq lthrnviaqv vmygllgpsv fdkddivict apvmhiagfw
      301 vvfgnlghgc klillhtldi glileaidrh kptmltlypt flakfeehpl lehvdttsvt
      361 klmtggslvs ssvlkkitlk lglkgviqvy gmtelgscia ystpemddfr sagkpgpfve
      421 ikvveentrk algpyqhgei cvkspgafks ylnnpkatae tyengfvrtg dlgyytpdgr
      481 fyvcsrlkdl ikcmdqqvap aeleellssd pevrqvvvvg vphpeygeaa rafvvprrll
      541 qpgsheeqre aqrlrdlvag rlaahkhlya gvefledipr tangknlrss lkeayvqlhc
      601 kcvkk
//
LOCUS       XP_077501353             117 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112377 [Amblyomma americanum].
ACCESSION   XP_077501353
VERSION     XP_077501353.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645227.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526547) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..117
                     /product="uncharacterized protein LOC144112377"
                     /calculated_mol_wt=12819
     Region          <10..99
                     /region_name="AFD_class_I"
                     /note="Adenylate forming domain, Class I superfamily;
                     cl17068"
                     /db_xref="CDD:473059"
     CDS             1..117
                     /gene="LOC144112377"
                     /coded_by="XM_077645227.1:1..354"
                     /db_xref="GeneID:144112377"
ORIGIN      
        1 matslfiypg dvgyynpdgr ffvcgrlkel tkcidqqvap aeleellssd sgvlhvvvvg
       61 vphheygeaa rafvvprqrl qpgsleeqre aqrlqdlvaa lappalyvyc rrnsfgg
//
LOCUS       XP_077501354             570 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112379 [Amblyomma americanum].
ACCESSION   XP_077501354
VERSION     XP_077501354.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645228.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526547) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..570
                     /product="uncharacterized protein LOC144112379"
                     /calculated_mol_wt=62731
     Region          106..>212
                     /region_name="SWIM"
                     /note="Uncharacterized protein, contains SWIM-type Zn
                     finger domain [Function unknown]; COG4715"
                     /db_xref="CDD:443750"
     Region          307..498
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(362,401,415,417)
                     /site_type="active"
                     /db_xref="CDD:411747"
     Region          521..561
                     /region_name="PHD_SF"
                     /note="PHD finger superfamily; cl22851"
                     /db_xref="CDD:473978"
     Site            order(521,532..535,539,556)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:276966"
     CDS             1..570
                     /gene="LOC144112379"
                     /coded_by="XM_077645228.1:123..1835"
                     /db_xref="GeneID:144112379"
ORIGIN      
        1 mssdyfkkld teaqvryrak lsfrgeelpd aldddvvrfa ftsgprnlpp vtvadiylyl
       61 vegvcfytne qfksykleda ynaficgkvk qvksfkagah gdgvvviaat veasqtlsks
      121 ykawcivked gtiesahctc maglgeccth vaallfrvea taqyglndps ptdvackwie
      181 askgstaapv seieffkpki grappqvsea pdysvpvltd eqvsrflhqv keiapntlil
      241 tsisdsedtd sapetaalep vrvgrckqls akpplaeiyq dldidegkkv eltaaccsei
      301 eeatrgqars prwlqerrgr itasvmhrva acstgaaglv aeimgyvktp qvsnlrwgse
      361 tekkavqafi etesqkhvdf slrdcglfia rsmpfigasp dalvscsccp ksvlevkcpa
      421 tmkdasltkg ltklaylned lqlkhnhayy tqiqaqmalt glcqayfvvf tgssltkeii
      481 sfdetfwaat klkaanffft yifpelqsmh ifsqiecakk tchcrgtklg hivecslcea
      541 tfhlkcvklr rtprqwicta cqhngqtade
//
LOCUS       XP_077501355             119 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112380 [Amblyomma americanum].
ACCESSION   XP_077501355
VERSION     XP_077501355.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645229.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526547) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..119
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..119
                     /product="uncharacterized protein LOC144112380"
                     /calculated_mol_wt=12894
     Region          46..>95
                     /region_name="BEN"
                     /note="The BEN domain is found in diverse animal proteins;
                     cl11158"
                     /db_xref="CDD:472128"
     CDS             1..119
                     /gene="LOC144112380"
                     /coded_by="XM_077645229.1:14..373"
                     /db_xref="GeneID:144112380"
ORIGIN      
        1 mlddmksida isavrpcpqi digggvliee taleklrssc pgapakfarg lmrqlftade
       61 lrgkslfgrk snshpdsslr ealdpvrvna iigftirefk adpirlkssl ssllsreik
//
LOCUS       XP_077501356             755 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112381 [Amblyomma americanum].
ACCESSION   XP_077501356
VERSION     XP_077501356.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645230.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526547) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..755
                     /product="uncharacterized protein LOC144112381"
                     /calculated_mol_wt=84062
     Region          5..86
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..755
                     /gene="LOC144112381"
                     /coded_by="XM_077645230.1:848..3115"
                     /db_xref="GeneID:144112381"
ORIGIN      
        1 mptvclvrqc rttyengsnv rfhklpgdpq rralwlrain rdmgsqtgyv csehflpgdy
       61 etnldvlrsl gmdlknarik rdaiptqnls pgappkkrrr seelpepqdv eailtaeeaq
      121 tspqmvdsct qtprntftva cqtdgrdvrt rytqvnlhis vrkdihvqtd agqttsahce
      181 actntstapa sttqpvsslp pppcqtvpsl pidgrapspi sfaprqsssp ieseasdcee
      241 ehntlqssef fpslnetflg ldssteevlp qddkkfiife sclkelfqvc rkcfaacksv
      301 shvsgtwlqi qtlcanhhfl twssqpming kaagnvlmsa amlfagtspt svlrmfnyvn
      361 vqvftprtfy nyqrgyllpa idkiwqqqqe elfrqlegct vdlagdgrcd spgfcakflt
      421 yslhvaqlnk ilhfeqvqvg ecadvqssts mekfafvksl nmvkerglkv asvttdrhgq
      481 vtkymrtqep tirhyydcwh iskgikkkla ahakragcsv lkiwiqpasn hlywcaalsd
      541 gsaelltdmw ksmerhaani htghpglyth cahdelgere wlvpgttahn kfvevvsspr
      601 ilkdirqlap cthtfsleaf hsvligfapk svcfspegmr artqlailhf nenaskgqat
      661 taeglsrwki khpkarkgta vacpvkaept ydyvrlllke tvhcceqwps fkaafaenls
      721 tapppmsrsf prpsknelva arrsrfassm gsttv
//
LOCUS       XP_077501357             204 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112383 [Amblyomma americanum].
ACCESSION   XP_077501357
VERSION     XP_077501357.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645231.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526547) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..204
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..204
                     /product="uncharacterized protein LOC144112383"
                     /calculated_mol_wt=22566
     Region          <71..198
                     /region_name="P2RX7_C"
                     /note="P2X purinoreceptor 7 intracellular domain;
                     pfam20478"
                     /db_xref="CDD:466627"
     CDS             1..204
                     /gene="LOC144112383"
                     /coded_by="XM_077645231.1:16..630"
                     /db_xref="GeneID:144112383"
ORIGIN      
        1 madlpelqrr llersrllgf epygdtplrv pdqpssedds ggsdngcedg argqqlavva
       61 rgttatfgsp dheppepgrv gnthwcfcgr cvsmptales tccreipaat vkqpsgcite
      121 hphfytlcld evvltvalqm lldhglrveh tgryryisys qlahwlwgrl grhhrkvlpa
      181 cavcqirerf psegytgfqy aqgl
//
LOCUS       XP_077501358             457 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112389 [Amblyomma americanum].
ACCESSION   XP_077501358
VERSION     XP_077501358.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645232.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526548) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..457
                     /product="uncharacterized protein LOC144112389"
                     /calculated_mol_wt=48494
     Region          <352..>454
                     /region_name="PHA02682"
                     /note="ORF080 virion core protein; Provisional"
                     /db_xref="CDD:177464"
     CDS             1..457
                     /gene="LOC144112389"
                     /coded_by="XM_077645232.1:159..1532"
                     /db_xref="GeneID:144112389"
ORIGIN      
        1 mppkpfavvk fpmeddslav vpvgwlshdw qhchwpkrra aevirlaree adpsapgcew
       61 frcpvavvtt crsyknaekk akkyemssav essaisgneg pdgpdvrlps ppprlpsppp
      121 rlpsppprlp spppqlpspp rlisppslls shsmeaatpa ganctagcpa gsastagdle
      181 dveeatsdpt mlkvlrllle irqqqreilq rvsrleqarq eprtrspspp vsslpplcpq
      241 lpafsiedfe aaeaavrddr vaaalkkqll rlggnnlkev aanvmgavmg vavqrlfslr
      301 grkgkrpfvg tklcrvatda icerqgvdil aaqgfigrwl pgacdrgggr krryaqtlep
      361 peshaqappa npcagsapcp gapsascpat ppgmailsps gvhpssapph sllltpshpp
      421 pklcpfpcpp ptasssrpat prstlatwvi aprahek
//
LOCUS       XP_077501359             695 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112392 [Amblyomma americanum].
ACCESSION   XP_077501359
VERSION     XP_077501359.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645233.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526548) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..695
                     /product="uncharacterized protein LOC144112392"
                     /calculated_mol_wt=77723
     CDS             1..695
                     /gene="LOC144112392"
                     /coded_by="XM_077645233.1:79..2166"
                     /db_xref="GeneID:144112392"
ORIGIN      
        1 msmetevmsk wrryriieke yrellgelgi rrppsthlks sgskrtggea aldvvsappl
       61 plpsgaskrp nseayasgac asgsgratss lpadpsmpsv qselneqayv sescaeslss
      121 ehtatscdss detsstnslt aenvemgqpr eespelcyrh lstaqelasi askhnlthac
      181 indlldfcrr rvivelpkda rtimqterka nlehgdsfvh fglaegiqha vgqgpapenv
      241 elhanidglp lhrssqtalw pilcrvinik ksepfmvsay cgagkppclq eylrpfidav
      301 rnlscnglmv kgvhvnvclk avicdapars fvkaitghtg yhacercsqk ghylenrmtf
      361 pdlhapqrtd ssfrsqedrr hhtgvspfts lnvdmvkcfp teymhlvclg vmrrllrnwi
      421 ckghaarlsr adrsalndkl reaatafpky fqrkprgtee ldrwkatefr tfllylgpvl
      481 lksilpdely khflylhvsv rvlaspqhhr dlnqfahdll ryfvqefgrl ygqkqlvynv
      541 htlahlaeqc lehgtvdefs afpfesylgk vkkrlrttnk plaqlsrrms evractplst
      601 gqvggdvkvg dcflvdncav vivdllggna kagilpngre ffrvpidssr mdvrrysalg
      661 qetkiwpisy iknkvrcikl qykrghvvfp llhlq
//
LOCUS       XP_077501360             304 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112393 [Amblyomma americanum].
ACCESSION   XP_077501360
VERSION     XP_077501360.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645234.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526548) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..304
                     /product="uncharacterized protein LOC144112393"
                     /calculated_mol_wt=31568
     CDS             1..304
                     /gene="LOC144112393"
                     /coded_by="XM_077645234.1:224..1138"
                     /db_xref="GeneID:144112393"
ORIGIN      
        1 meaatpagan ctagcpagsa stagdledve eatsdptmlk vlrllleirq qqreilqrvs
       61 rleqarqepr trspsppvss lpplcpqlpa fsiedfeaae aavrddrvaa alkkqllrlg
      121 gnnlkevaan vmgavmgvav qrlfslrgrk gkrpfvgtkl crvatdaice rqgvdilaaq
      181 gfigrwlpga cdrgggrkrr yaqaleppgs haqappanpc agsapcpgap sascpatppg
      241 mailspsgvh pssappqppp haqppstqal plplppppta sssrpatprs tlatwviapr
      301 ahek
//
LOCUS       XP_077501361             599 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112394 [Amblyomma americanum].
ACCESSION   XP_077501361
VERSION     XP_077501361.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645235.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526548) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..599
                     /product="uncharacterized protein LOC144112394"
                     /calculated_mol_wt=67566
     Region          141..284
                     /region_name="Transposase_21"
                     /note="Transposase family tnp2; cl29371"
                     /db_xref="CDD:475188"
     CDS             1..599
                     /gene="LOC144112394"
                     /coded_by="XM_077645235.1:186..1985"
                     /db_xref="GeneID:144112394"
ORIGIN      
        1 mpsvqselne qayvsescae slssehtats cnssdetsst nsltaenvem gqpreespel
       61 cyrhlstaqe lasiaskhnl thacindlld fcrrrgivel pkdartimqt krkanlehgd
      121 sfvhfglaeg iqhavgqgpa penvelhani dglplhrssq talwpilcrv inikksepfm
      181 vsaycgagkp pclqeylrpf idevrnlscn glmvkgvhvn vclkavicda parsfvkait
      241 ghtgyhacer csqkghylen rmtfpdlhap qrtdssfrsq edrrhhtgvs pftslnvdmv
      301 kcfpteymhl vclgvmrrll rnwickghaa rlsradrsal ndklreaata fpkyfqrkpr
      361 gteeldrwka tefrtfllyl gpvllksilp delykhflyl hvsvrvlasp qhhrdlnqfa
      421 hdllryfvqe fgrlygqkql vynvhtlahl aeqclehgtv defsafpfes ylgkvkkrlr
      481 ttnkplaqls rrmsevract plstgqvggd vkvgdcflvd ncavvivdll ggnakagilp
      541 ngreffrvpi dssrmdvrry salgqetkiw pisyiknkvr ciklqykrgh vvfpllhlq
//
LOCUS       XP_077501363             115 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112395 [Amblyomma americanum].
ACCESSION   XP_077501363
VERSION     XP_077501363.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645237.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526550) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..115
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..115
                     /product="uncharacterized protein LOC144112395"
                     /calculated_mol_wt=12530
     CDS             1..115
                     /gene="LOC144112395"
                     /coded_by="XM_077645237.1:291..638"
                     /db_xref="GeneID:144112395"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrppfa altsvlccqr pasigpcvlp afgcvippdp
       61 fmlktkshme vvqatklrvi fardleqagk lvdgqwysls kasffckcsf whgia
//
LOCUS       XP_077501364             146 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112397 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501364
VERSION     XP_077501364.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645238.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526554) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..146
                     /product="uncharacterized protein LOC144112397 isoform X1"
                     /calculated_mol_wt=15897
     CDS             1..146
                     /gene="LOC144112397"
                     /coded_by="XM_077645238.1:283..723"
                     /db_xref="GeneID:144112397"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdptrrppfa altsvlccqr pasigpcvlp afdcvippdp
       61 fmlktkscme vlqatklrvi fardleqagk lvdgqwysls nasffsktkt iflgswamyl
      121 gtcgqdnagq hasasskkkh etvnky
//
LOCUS       XP_077501365             140 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112397 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501365
VERSION     XP_077501365.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645239.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526554) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..140
                     /product="uncharacterized protein LOC144112397 isoform X2"
                     /calculated_mol_wt=15257
     CDS             1..140
                     /gene="LOC144112397"
                     /coded_by="XM_077645239.1:283..705"
                     /db_xref="GeneID:144112397"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdptrrppfa altsvlccqr pasigpcvlp afdcvippdp
       61 fmlktkscme vlqatklrvi fardleqagk lvdgqwyslt ktktiflgsw amylgtcgqd
      121 nagqhasass kkkhetvnky
//
LOCUS       XP_077501366            1090 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112398 [Amblyomma americanum].
ACCESSION   XP_077501366
VERSION     XP_077501366.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645240.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526560) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1090
                     /product="uncharacterized protein LOC144112398"
                     /calculated_mol_wt=123010
     Region          237..293
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          <297..464
                     /region_name="transpos_IS481"
                     /note="IS481 family transposase; NF033577"
                     /db_xref="CDD:468094"
     Region          701..875
                     /region_name="RT_LTR"
                     /note="Reverse transcriptases (RTs) from retrotransposons
                     and retroviruses which have long terminal repeats (LTRs)
                     in their DNA copies but not in their RNA template. RT
                     catalyzes DNA replication from an RNA template, and is
                     responsible for the replication of...; cd01647"
                     /db_xref="CDD:238825"
     Site            order(764..769,798..799,824,826..827,871..872)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238825"
     Site            order(764..769,798,826)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238825"
     Site            799
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238825"
     Region          971..1089
                     /region_name="RNase_HI_RT_Ty3"
                     /note="Ty3/Gypsy family of RNase HI in long-term repeat
                     retroelements; cd09274"
                     /db_xref="CDD:260006"
     Site            order(975..978,1011..1013,1016,1041,1074,1088)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260006"
     CDS             1..1090
                     /gene="LOC144112398"
                     /coded_by="XM_077645240.1:1..3273"
                     /db_xref="GeneID:144112398"
ORIGIN      
        1 mpvvegeihg qritvlrdsg tntdlvrrsl vgkkeltgrl asvllvdstv rylpeamici
       61 stpyysgpvv vkcldeplyd lvlgnitgvr kvddpdadwk psmeqgagpe grseeetlvm
      121 tranvvpreg ngglepaegk rdetpmissa vqtragkaaq gktaklkvpa aellkvtpes
      181 laqeqrndss ladcfkkvge ilrrkkcrtt fqflvqdgll yrkctfssgr evlqlvipke
      241 lrpavmclgh dsimaghqga kntgnrinee fywpgvqsdv kryvrscdic qrtipkgkvg
      301 kaplgtmpti eipfqrvavd iigpitpssk kgnryvitlv dvatrypdai alktidttev
      361 aeallemfsr ygvpkemltd rgsnftsdlm kevsrlmslk qllttpyhpm cnglverfng
      421 tikhmmkkmc qerpadwdry lpallfayre vpqsslgfsp femlygravr gpltilrelw
      481 snnkletdqk ttytyvlelr erleetceia hrnldasker ykkfynrksv nrqlrpgera
      541 lillpseknk ltmqwkgpfp vtgkknevdy ellvsgkkki fhinmlkrye ertanadqmi
      601 scmviseesd agevptfnsr ktmgpedvvl nptldagqta qlkcllkryg tvfsdlpgkt
      661 kiiacklrlt teqpvhvrqy plpliahksv ekeveemlrl giiertesay nsplivvknt
      721 dgsnrlcvdf rrlnsvlipd sepipridvv fasvaqkkyf skfdlakgyw qipmeesske
      781 ktafssangl fqfrfmpfgl ktaaatftkl mrqvlgglhq vhhyiddilv atdtweehlm
      841 aletlferlr eanltvkptk cevgfesvsf lgheigmgri atkpdiveki qaaprpttkk
      901 qvqsflgltg fyrefiphya dmalplvett kkgapntvnw sdrqqeafnk lkesmaqppv
      961 llapnvnkqf vlrtdasgta lgavlmqeed gilhpvayas kklslceqny stvekeclal
     1021 vwaikkfhly lyethfrvqt dhqplqcltk tkssngrlmr wsltlqeyef hveyikgren
     1081 lgadflsrln
//
LOCUS       XP_077501367             294 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112399 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501367
VERSION     XP_077501367.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645241.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526562) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..294
                     /product="uncharacterized protein LOC144112399 isoform X1"
                     /calculated_mol_wt=33191
     Region          113..161
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          194..>278
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..294
                     /gene="LOC144112399"
                     /coded_by="XM_077645241.1:995..1879"
                     /db_xref="GeneID:144112399"
ORIGIN      
        1 maadqqnssl sreetemkvi ykkmdsqnni kdcllfqvek kdnstqwele epvpdhtyad
       61 tndssntplt mrsihfqylt pqdvhfytgl pfdafnklil llrangtkvg gilgleqqll
      121 lthmrlrlgl lcgdlarrfs isiatvstif ssmmstlakv medivvwlpr svvydsmpyt
      181 fvkngydrtt cifdcteawl qrpkklmara qsysaykgss tikfltviap nglimfvsdv
      241 yggrasdkyi vrtcgvedyl lpgdeimadr gfklephlea qgirmnvpaf tram
//
LOCUS       XP_077501368             294 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112399 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501368
VERSION     XP_077501368.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645242.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526562) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..294
                     /product="uncharacterized protein LOC144112399 isoform X1"
                     /calculated_mol_wt=33191
     Region          113..161
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          194..>278
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..294
                     /gene="LOC144112399"
                     /coded_by="XM_077645242.1:786..1670"
                     /db_xref="GeneID:144112399"
ORIGIN      
        1 maadqqnssl sreetemkvi ykkmdsqnni kdcllfqvek kdnstqwele epvpdhtyad
       61 tndssntplt mrsihfqylt pqdvhfytgl pfdafnklil llrangtkvg gilgleqqll
      121 lthmrlrlgl lcgdlarrfs isiatvstif ssmmstlakv medivvwlpr svvydsmpyt
      181 fvkngydrtt cifdcteawl qrpkklmara qsysaykgss tikfltviap nglimfvsdv
      241 yggrasdkyi vrtcgvedyl lpgdeimadr gfklephlea qgirmnvpaf tram
//
LOCUS       XP_077501369             294 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112399 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501369
VERSION     XP_077501369.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645243.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526562) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..294
                     /product="uncharacterized protein LOC144112399 isoform X1"
                     /calculated_mol_wt=33191
     Region          113..161
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          194..>278
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..294
                     /gene="LOC144112399"
                     /coded_by="XM_077645243.1:920..1804"
                     /db_xref="GeneID:144112399"
ORIGIN      
        1 maadqqnssl sreetemkvi ykkmdsqnni kdcllfqvek kdnstqwele epvpdhtyad
       61 tndssntplt mrsihfqylt pqdvhfytgl pfdafnklil llrangtkvg gilgleqqll
      121 lthmrlrlgl lcgdlarrfs isiatvstif ssmmstlakv medivvwlpr svvydsmpyt
      181 fvkngydrtt cifdcteawl qrpkklmara qsysaykgss tikfltviap nglimfvsdv
      241 yggrasdkyi vrtcgvedyl lpgdeimadr gfklephlea qgirmnvpaf tram
//
LOCUS       XP_077501370             275 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112399 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501370
VERSION     XP_077501370.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645244.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526562) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..275
                     /product="uncharacterized protein LOC144112399 isoform X2"
                     /calculated_mol_wt=30909
     Region          94..142
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          175..>259
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..275
                     /gene="LOC144112399"
                     /coded_by="XM_077645244.1:1255..2082"
                     /db_xref="GeneID:144112399"
ORIGIN      
        1 maadqqnssl sreetemkve kkdnstqwel eepvpdhtya dtndssntpl tmrsihfqyl
       61 tpqdvhfytg lpfdafnkli lllrangtkv ggilgleqql llthmrlrlg llcgdlarrf
      121 sisiatvsti fssmmstlak vmedivvwlp rsvvydsmpy tfvkngydrt tcifdcteaw
      181 lqrpkklmar aqsysaykgs stikfltvia pnglimfvsd vyggrasdky ivrtcgvedy
      241 llpgdeimad rgfklephle aqgirmnvpa ftram
//
LOCUS       XP_077501371             830 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112404 [Amblyomma americanum].
ACCESSION   XP_077501371
VERSION     XP_077501371.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645245.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526565) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..830
                     /product="uncharacterized protein LOC144112404"
                     /calculated_mol_wt=89177
     Region          172..263
                     /region_name="FHA"
                     /note="forkhead associated (FHA) domain superfamily;
                     cl00062"
                     /db_xref="CDD:469597"
     Region          <381..>828
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..830
                     /gene="LOC144112404"
                     /coded_by="XM_077645245.1:137..2629"
                     /db_xref="GeneID:144112404"
ORIGIN      
        1 mdcsqspset sasvrragep aavsgepvsk gyvqsrssqs lllfqkdadg asptgstlnq
       61 acshasgfim vqsdhtyagp steasassle pgtagvaatl sasadlspga pfsssplsce
      121 gsftspiner rplqklrakl rqlrnrnkrl qglllqrrrm kttaelmeyy pkivtlrrng
      181 tgfsvlplit grcsvggaag cdiriprsga ycclflvgkn eqahiincsg tpdlklngkd
      241 vhdhsvlshg divqisyrkf rfdygekrkk ensskvaaas hqpkaagdat ptsnnkrkka
      301 steqagavac eegddeeevv llqsardisq scqeslehms vvaespkvtv skqtfqyssq
      361 ileisresap ekatlptrsg rssqtssneg klkvqekeke atvgsnqkps skpskakrtp
      421 askrataadv ttelekagms asknlafdnt dvtaaafgra traarpkyvg tdkrtdvkmp
      481 rqeakrtvsp lktleaeqrp asnkelgetp kqeaadtkks srkilqakta rstrkvnege
      541 eapelgtagv spskcssatn vhvldeparp trgcrtrgsr kdiveapkek kvdqlqstka
      601 traehtqgsr kvckegetlq dvtgsslvma sttqrkdgtf dkhlqgeasp tkevsvtrnt
      661 rakrkvdlke esiaekhtta edligtvaek sgghqqrvas geyapqendv qcsnsakqvi
      721 npkitrdkev vddapaspnv evatvepasa ggkalattme pkrskrtmaa vkvdvaqtan
      781 eatprktrgr raealktale waddkpgasr rtakdrseee pppkrtrkmv
//
LOCUS       XP_077501372             122 aa            linear   INV 25-NOV-2025
DEFINITION  lysosomal alpha-glucosidase-like [Amblyomma americanum].
ACCESSION   XP_077501372
VERSION     XP_077501372.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645246.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526565) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 46% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..122
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..122
                     /product="lysosomal alpha-glucosidase-like"
                     /calculated_mol_wt=14110
     CDS             1..122
                     /gene="LOC144112405"
                     /coded_by="XM_077645246.1:5..373"
                     /db_xref="GeneID:144112405"
ORIGIN      
        1 mkllvalngs deavgdlywd ggetldaqll geyvslkfra annsleicsy qykklfnstl
       61 relaimsvra lgicwrptrv eqdgyyhish rqcrwhhsnk vmdlkqilmp lrknttvrwf
      121 ms
//
LOCUS       XP_077501373             174 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112408 [Amblyomma americanum].
ACCESSION   XP_077501373
VERSION     XP_077501373.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645247.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526565) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..174
                     /product="uncharacterized protein LOC144112408"
                     /calculated_mol_wt=19601
     Region          <8..>88
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..174
                     /gene="LOC144112408"
                     /coded_by="XM_077645247.1:1..525"
                     /db_xref="GeneID:144112408"
ORIGIN      
        1 mmyskawilq ciimqmkspr lyehmrrqni ltlpskvtlq rylksyrtgf gfnenvlnil
       61 kqktstmdif krhgglivde mklsenlcvs ssghiqgfvd rskftprsem hnvadhetvi
      121 mfvpfagkwt hilacfatrg ntkgdlltkv mleavilaen aglfvdvias dgat
//
LOCUS       XP_077501374             221 aa            linear   INV 25-NOV-2025
DEFINITION  casein kinase II subunit beta-like [Amblyomma americanum].
ACCESSION   XP_077501374
VERSION     XP_077501374.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645248.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526565) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..221
                     /product="casein kinase II subunit beta-like"
                     /calculated_mol_wt=25228
     Region          8..191
                     /region_name="CK_II_beta"
                     /note="Casein kinase II regulatory subunit; smart01085"
                     /db_xref="CDD:198153"
     CDS             1..221
                     /gene="LOC144112412"
                     /coded_by="XM_077645248.1:51..716"
                     /db_xref="GeneID:144112412"
ORIGIN      
        1 mssseevswi srfcglrgne ffcevdedyi qdevnltgld eqvphyrqal dmildlepdd
       61 emddnpnqsd lieqaaekly gliharyilt nrgmalmiek yqngdfgycp rvycesqkml
      121 piglsdvpge vmvklycpkc mdvygpkssr hhrtdgayfg tgfpnmlfma hpeyrpkspk
      181 nefvprlygf kihplaykqe kqeaanfkap igamnsgnen r
//
LOCUS       XP_077501375             221 aa            linear   INV 25-NOV-2025
DEFINITION  casein kinase II subunit beta-like [Amblyomma americanum].
ACCESSION   XP_077501375
VERSION     XP_077501375.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645249.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526565) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..221
                     /product="casein kinase II subunit beta-like"
                     /calculated_mol_wt=25228
     Region          8..191
                     /region_name="CK_II_beta"
                     /note="Casein kinase II regulatory subunit; smart01085"
                     /db_xref="CDD:198153"
     CDS             1..221
                     /gene="LOC144112412"
                     /coded_by="XM_077645249.1:51..716"
                     /db_xref="GeneID:144112412"
ORIGIN      
        1 mssseevswi srfcglrgne ffcevdedyi qdevnltgld eqvphyrqal dmildlepdd
       61 emddnpnqsd lieqaaekly gliharyilt nrgmalmiek yqngdfgycp rvycesqkml
      121 piglsdvpge vmvklycpkc mdvygpkssr hhrtdgayfg tgfpnmlfma hpeyrpkspk
      181 nefvprlygf kihplaykqe kqeaanfkap igamnsgnen r
//
LOCUS       XP_077501377             221 aa            linear   INV 25-NOV-2025
DEFINITION  casein kinase II subunit beta-like [Amblyomma americanum].
ACCESSION   XP_077501377
VERSION     XP_077501377.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645251.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526565) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..221
                     /product="casein kinase II subunit beta-like"
                     /calculated_mol_wt=25228
     Region          8..191
                     /region_name="CK_II_beta"
                     /note="Casein kinase II regulatory subunit; smart01085"
                     /db_xref="CDD:198153"
     CDS             1..221
                     /gene="LOC144112412"
                     /coded_by="XM_077645251.1:51..716"
                     /db_xref="GeneID:144112412"
ORIGIN      
        1 mssseevswi srfcglrgne ffcevdedyi qdevnltgld eqvphyrqal dmildlepdd
       61 emddnpnqsd lieqaaekly gliharyilt nrgmalmiek yqngdfgycp rvycesqkml
      121 piglsdvpge vmvklycpkc mdvygpkssr hhrtdgayfg tgfpnmlfma hpeyrpkspk
      181 nefvprlygf kihplaykqe kqeaanfkap igamnsgnen r
//
LOCUS       XP_077501378             225 aa            linear   INV 25-NOV-2025
DEFINITION  casein kinase II subunit beta-like [Amblyomma americanum].
ACCESSION   XP_077501378
VERSION     XP_077501378.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645252.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526565) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..225
                     /product="casein kinase II subunit beta-like"
                     /calculated_mol_wt=25408
     Region          12..195
                     /region_name="CK_II_beta"
                     /note="Casein kinase II regulatory subunit; smart01085"
                     /db_xref="CDD:198153"
     CDS             1..225
                     /gene="LOC144112413"
                     /coded_by="XM_077645252.1:101..778"
                     /db_xref="GeneID:144112413"
ORIGIN      
        1 mseiisssee vswisrfcgl rgneffcevd edyiqdevnl igldeqvphy rqaldiildl
       61 etddemddnp nqsdlieqaa emlyglihar yiltnrgial miekyrngdf gycprvyces
      121 qqmlpigmtd vpgeamvkly cpkcmdvygp kssrhhhtdg ayfgtgfphm lfmvhpeyrp
      181 ksptnhlvpr lngfkihpla dqpqqqvaan fkapigamnf gngkg
//
LOCUS       XP_077501379             131 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112414 [Amblyomma americanum].
ACCESSION   XP_077501379
VERSION     XP_077501379.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645253.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526567) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..131
                     /product="uncharacterized protein LOC144112414"
                     /calculated_mol_wt=14561
     Region          3..>89
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     CDS             1..131
                     /gene="LOC144112414"
                     /coded_by="XM_077645253.1:1..396"
                     /db_xref="GeneID:144112414"
ORIGIN      
        1 mqpisltscv gkmmermvrr rlqkrpggtd qmletmygfk qhlstqdvmi klhemvakpa
       61 trhaprgila hdlkgafdnv sdatvlqsln ktrcgrktfg yikdflsnrt atisigeeks
      121 dpvelgergt p
//
LOCUS       XP_077501380             229 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112416 [Amblyomma americanum].
ACCESSION   XP_077501380
VERSION     XP_077501380.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645254.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526568) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..229
                     /product="uncharacterized protein LOC144112416"
                     /calculated_mol_wt=26651
     Region          <28..76
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..229
                     /gene="LOC144112416"
                     /coded_by="XM_077645254.1:1..690"
                     /db_xref="GeneID:144112416"
ORIGIN      
        1 mpsfylllld yyaryardvg plkkfrskra mfeqiacdmf gilgihrtav qcetrfktvs
       61 krkrneekhn ntsgnsrcrv eyeqefdaik aiddslepev lrgvdtvryk kastrkaspr
      121 pstsadvqen sefsvdtlsl issggdcsfe agtdsqdeep kkkradrrlr psnsrmqhmq
      181 lffeemnklh rekekkkedr eqrkeqrhqe llkahsehma llktlaeke
//
LOCUS       XP_077501381             257 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112417 [Amblyomma americanum].
ACCESSION   XP_077501381
VERSION     XP_077501381.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645255.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526568) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..257
                     /product="uncharacterized protein LOC144112417"
                     /calculated_mol_wt=29088
     Region          26..77
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          107..242
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..257
                     /gene="LOC144112417"
                     /coded_by="XM_077645255.1:1..774"
                     /db_xref="GeneID:144112417"
ORIGIN      
        1 mcrlpskslf lffvsrfenv alyipnrctv adavqvvltk lrlallnidi ihrfglsett
       61 vprimvknip iiaselkrfi iwpsrkdacr tmpmmvrrky ptcmaiidct evriqkllgy
      121 strsktyshy kssntikfmv sitpcgaisf vtkcwggras dkeiilksgf sdklqegdiv
      181 ladrgflird vahrgarlev paltrgkrql sakevedarq isrvrihver aigrikvfhi
      241 lradcpslcc gmqqtll
//
LOCUS       XP_077501383             156 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112420 [Amblyomma americanum].
ACCESSION   XP_077501383
VERSION     XP_077501383.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645257.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526569) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 28% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..156
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..156
                     /product="uncharacterized protein LOC144112420"
                     /calculated_mol_wt=16965
     CDS             1..156
                     /gene="LOC144112420"
                     /coded_by="XM_077645257.1:1..471"
                     /db_xref="GeneID:144112420"
ORIGIN      
        1 mkgnthrmlm tcrkkkkqrk eamlslhegg dfhvegvivd ssevlsatqa edtassgdgd
       61 gttslddtdr hgsispleer qaerghaglh ddewhcahar rraaaagfcl lcclvflssa
      121 avtclvmlvl rndqrisalf srlidaalel rlgnyd
//
LOCUS       XP_077501384             142 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112421 [Amblyomma americanum].
ACCESSION   XP_077501384
VERSION     XP_077501384.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645258.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526569) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 32% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..142
                     /product="uncharacterized protein LOC144112421"
                     /calculated_mol_wt=15361
     CDS             1..142
                     /gene="LOC144112421"
                     /coded_by="XM_077645258.1:13..441"
                     /db_xref="GeneID:144112421"
ORIGIN      
        1 mthvfvkwqe ekwdvyaira ldadvgmqll akesaieelr gimvmvewea sqppasatll
       61 gfgteramew qrtrlaleae vgavpntpet esgtqpptkr qknkivqnev sdigraagrq
      121 ctgdsvlsgi gggycdppis gf
//
LOCUS       XP_077501385             120 aa            linear   INV 25-NOV-2025
DEFINITION  evasin P1243-like [Amblyomma americanum].
ACCESSION   XP_077501385
VERSION     XP_077501385.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645259.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526569) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..120
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..120
                     /product="evasin P1243-like"
                     /calculated_mol_wt=13024
     Region          43..116
                     /region_name="EVA_Class_A"
                     /note="Evasins Class A; pfam19429"
                     /db_xref="CDD:437261"
     CDS             1..120
                     /gene="LOC144112422"
                     /coded_by="XM_077645259.1:48..410"
                     /db_xref="GeneID:144112422"
ORIGIN      
        1 mhltiayvcv salalfvaln grsraenydg nlteydynee cscpsphlnn tngtfpkplg
       61 chyfcnvtlc tapdgyscyn ltaqqvrtlt typktscavg vckkgtcvkn gtmeqcfktp
//
LOCUS       XP_077501386             657 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112423 [Amblyomma americanum].
ACCESSION   XP_077501386
VERSION     XP_077501386.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645260.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526572) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..657
                     /product="uncharacterized protein LOC144112423"
                     /calculated_mol_wt=71554
     Region          <483..629
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..657
                     /gene="LOC144112423"
                     /coded_by="XM_077645260.1:302..2275"
                     /db_xref="GeneID:144112423"
ORIGIN      
        1 mdnaerlgda nstpaalpeh kddaalaeqp psleaagaid sgdcnadcsp siqravtspl
       61 vdlfkddssg rhlaingnnk pseaqsdrsn pctveptlle tcmvpangdv kngprkiims
      121 aelhvcgags akeesqpkga clfsrpeapg griafhades pdtvmrnqfs cdsdlgssnp
      181 fkgdvlnitp sgsdkleavn ddaakspvtg navakvpplr svrpaagdkl tkagapagss
      241 rncsqlamde macvsdqlll vdtctqapve knlpaeqnkg nevkqkpstv cpklslcglq
      301 peelrrktyq llrrqrdeay dhvgrlekei allqkfydrh cefllnklrk legenrrlsa
      361 anavqrrlmq ellprlgdss lpgtvsdqlk lqgeggakpp lhsvtpaggd elikagspag
      421 sshnsrqlav gemaclsdkl lpvdsgtpas vqenlhaehn ngknvkqkpg vvcpnlslca
      481 qpaeelrrqr aqaydrverl ekeivllrnf ydrhseflln kvrklneknr tlsatnaeqr
      541 llmqellsrr gnsslpdair eqeelqaevk rlrkqillre eelqdlkanf lrmrkasten
      601 yraathdink vdtmlqrvhe afeaafddvc rspalkqlll nlrsggggwk dttpssk
//
LOCUS       XP_077501387             481 aa            linear   INV 25-NOV-2025
DEFINITION  venom metalloproteinase antarease TserMP_A-like [Amblyomma
            americanum].
ACCESSION   XP_077501387
VERSION     XP_077501387.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645261.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526581) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..481
                     /product="venom metalloproteinase antarease TserMP_A-like"
                     /calculated_mol_wt=54173
     Region          162..370
                     /region_name="ZnMc"
                     /note="Zinc-dependent metalloprotease. This super-family
                     of metalloproteases contains two major branches, the
                     astacin-like proteases and the adamalysin/reprolysin-like
                     proteases. Both branches have wide phylogenetic
                     distribution, and contain sub-families, which...; cl00064"
                     /db_xref="CDD:469599"
     Site            order(302..303,306,312)
                     /site_type="active"
                     /db_xref="CDD:239033"
     CDS             1..481
                     /gene="LOC144112427"
                     /coded_by="XM_077645261.1:60..1505"
                     /db_xref="GeneID:144112427"
ORIGIN      
        1 mlminehisl nlqktspfte rfslvyekdg iqqveersah slsgelykdp dneaailisr
       61 rdglrlkgvi keniidhddd avlqdgavrh rlsarenpae knyhgddlay tseaserflg
      121 rfqerrdiam sersfeskgf lkvtvrtsvl msqeyivgfk rsnysavatv idyvavffas
      181 vnnflekfdk ktldlqlsvt slvlltksse tfvqvlpser tvingstlvk lnqfadsrrd
      241 ligkddiviy lskytistdq asgpktpraa gfaslggact rnrsalvqdn pktfagvftt
      301 vhetlhligv vhegdaapry lennlgaqdc sgystsimap vmqhtelkls hctqrqvlay
      361 lrsrrgeclr lrnpnrhqnd ikrnliravl vntsnlckkm vpdkpnvkyv ekldekhnmg
      421 tcyiicrwve gdsayyqytk apdftpcgkn qhgplkacly gkcseisthm ktfhkkikkn
      481 l
//
LOCUS       XP_077501389             161 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112430 [Amblyomma americanum].
ACCESSION   XP_077501389
VERSION     XP_077501389.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645263.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526585) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..161
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..161
                     /product="uncharacterized protein LOC144112430"
                     /calculated_mol_wt=17614
     CDS             1..161
                     /gene="LOC144112430"
                     /coded_by="XM_077645263.1:586..1071"
                     /db_xref="GeneID:144112430"
ORIGIN      
        1 mfrrlpnggn lkdvpvhsgv ledqneqtpt delpgtpqqc vplpkvtdsm etpagrelsg
       61 isassaddiv agqhchgteh sprhgdpvrs ipaglaglhv cmkhcyresl hsqgfpeahl
      121 envmksfnry iskeisdvqk takrwqskgc tcaqrrarrp k
//
LOCUS       XP_077501390             157 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112431 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501390
VERSION     XP_077501390.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645264.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526585) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..157
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..157
                     /product="uncharacterized protein LOC144112431 isoform X1"
                     /calculated_mol_wt=16951
     CDS             1..157
                     /gene="LOC144112431"
                     /coded_by="XM_077645264.1:230..703"
                     /db_xref="GeneID:144112431"
ORIGIN      
        1 mfrrlpnggn lkdvpvhsgv ledqneqtpt delpgtpqqc vplpkvtdsm etpagrelsg
       61 isassaddiv agqhchgteh sprhgdpvrs ipaglaglhv cmkpgivkca litqpaaasf
      121 lhlwhhclme qewanyntdn fqgsftmnyl elfschs
//
LOCUS       XP_077501391             157 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112431 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501391
VERSION     XP_077501391.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645265.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526585) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..157
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..157
                     /product="uncharacterized protein LOC144112431 isoform X1"
                     /calculated_mol_wt=16951
     CDS             1..157
                     /gene="LOC144112431"
                     /coded_by="XM_077645265.1:230..703"
                     /db_xref="GeneID:144112431"
ORIGIN      
        1 mfrrlpnggn lkdvpvhsgv ledqneqtpt delpgtpqqc vplpkvtdsm etpagrelsg
       61 isassaddiv agqhchgteh sprhgdpvrs ipaglaglhv cmkpgivkca litqpaaasf
      121 lhlwhhclme qewanyntdn fqgsftmnyl elfschs
//
LOCUS       XP_077501392             157 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112431 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501392
VERSION     XP_077501392.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645266.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526585) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..157
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..157
                     /product="uncharacterized protein LOC144112431 isoform X1"
                     /calculated_mol_wt=16951
     CDS             1..157
                     /gene="LOC144112431"
                     /coded_by="XM_077645266.1:187..660"
                     /db_xref="GeneID:144112431"
ORIGIN      
        1 mfrrlpnggn lkdvpvhsgv ledqneqtpt delpgtpqqc vplpkvtdsm etpagrelsg
       61 isassaddiv agqhchgteh sprhgdpvrs ipaglaglhv cmkpgivkca litqpaaasf
      121 lhlwhhclme qewanyntdn fqgsftmnyl elfschs
//
LOCUS       XP_077501393             157 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112431 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501393
VERSION     XP_077501393.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645267.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526585) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..157
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..157
                     /product="uncharacterized protein LOC144112431 isoform X1"
                     /calculated_mol_wt=16951
     CDS             1..157
                     /gene="LOC144112431"
                     /coded_by="XM_077645267.1:128..601"
                     /db_xref="GeneID:144112431"
ORIGIN      
        1 mfrrlpnggn lkdvpvhsgv ledqneqtpt delpgtpqqc vplpkvtdsm etpagrelsg
       61 isassaddiv agqhchgteh sprhgdpvrs ipaglaglhv cmkpgivkca litqpaaasf
      121 lhlwhhclme qewanyntdn fqgsftmnyl elfschs
//
LOCUS       XP_077501394             157 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112431 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501394
VERSION     XP_077501394.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645268.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526585) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..157
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..157
                     /product="uncharacterized protein LOC144112431 isoform X1"
                     /calculated_mol_wt=16951
     CDS             1..157
                     /gene="LOC144112431"
                     /coded_by="XM_077645268.1:124..597"
                     /db_xref="GeneID:144112431"
ORIGIN      
        1 mfrrlpnggn lkdvpvhsgv ledqneqtpt delpgtpqqc vplpkvtdsm etpagrelsg
       61 isassaddiv agqhchgteh sprhgdpvrs ipaglaglhv cmkpgivkca litqpaaasf
      121 lhlwhhclme qewanyntdn fqgsftmnyl elfschs
//
LOCUS       XP_077501395             151 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112431 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501395
VERSION     XP_077501395.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645269.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526585) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..151
                     /product="uncharacterized protein LOC144112431 isoform X2"
                     /calculated_mol_wt=16262
     CDS             1..151
                     /gene="LOC144112431"
                     /coded_by="XM_077645269.1:230..685"
                     /db_xref="GeneID:144112431"
ORIGIN      
        1 mfrrlpnggn lkdvpvhsgv ledqneqtpt delpgtpqqc vplpkvtdsm etpagrelsg
       61 isassaddiv agqhchgteh sprhgdpvrs ipaglaglhv cmkpgivkca litqpaaasf
      121 lhlwhhclme qewanyntdn fqgsftmnyl d
//
LOCUS       XP_077501396             132 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112431 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501396
VERSION     XP_077501396.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645270.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526585) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..132
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..132
                     /product="uncharacterized protein LOC144112431 isoform X3"
                     /calculated_mol_wt=14327
     CDS             1..132
                     /gene="LOC144112431"
                     /coded_by="XM_077645270.1:230..628"
                     /db_xref="GeneID:144112431"
ORIGIN      
        1 mfrrlpnggn lkdvpvhsgv ledqneqtpt delpgisass addivagqhc hgtehsprhg
       61 dpvrsipagl aglhvcmkpg ivkcalitqp aaasflhlwh hclmeqewan yntdnfqgsf
      121 tmnylelfsc hs
//
LOCUS       XP_077501397              94 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112431 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077501397
VERSION     XP_077501397.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645271.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526585) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..94
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..94
                     /product="uncharacterized protein LOC144112431 isoform X4"
                     /calculated_mol_wt=9826
     CDS             1..94
                     /gene="LOC144112431"
                     /coded_by="XM_077645271.1:230..514"
                     /db_xref="GeneID:144112431"
ORIGIN      
        1 mfrrlpnggn lkdvpvhsgv ledqneqtpt delpgtpqqc vplpkvtdsm etpagrelsg
       61 isassaddiv agqhchgteh sprhgdplal ssvh
//
LOCUS       XP_077501399              94 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112431 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077501399
VERSION     XP_077501399.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645273.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526585) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..94
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..94
                     /product="uncharacterized protein LOC144112431 isoform X4"
                     /calculated_mol_wt=9826
     CDS             1..94
                     /gene="LOC144112431"
                     /coded_by="XM_077645273.1:230..514"
                     /db_xref="GeneID:144112431"
ORIGIN      
        1 mfrrlpnggn lkdvpvhsgv ledqneqtpt delpgtpqqc vplpkvtdsm etpagrelsg
       61 isassaddiv agqhchgteh sprhgdplal ssvh
//
LOCUS       XP_077501400             212 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112433 [Amblyomma americanum].
ACCESSION   XP_077501400
VERSION     XP_077501400.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645274.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526595) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..212
                     /product="uncharacterized protein LOC144112433"
                     /calculated_mol_wt=24820
     CDS             1..212
                     /gene="LOC144112433"
                     /coded_by="XM_077645274.1:154..792"
                     /db_xref="GeneID:144112433"
ORIGIN      
        1 mssdqckvyi cftkvnpllr llslsescgc emwwskhwal afrykehiiv ydatningql
       61 qgskkwitek vfekayseke ciadptlpkq rplargdrad raaeedcial dswtghsrpv
      121 llvakllrvt tiperlldet lksmncleds tyrlnknncq twvklflrkl rdclprslfd
      181 dinlfffyqs pilvflsrcw tlprrrfsrd ei
//
LOCUS       XP_077501401             468 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112434 [Amblyomma americanum].
ACCESSION   XP_077501401
VERSION     XP_077501401.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645275.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526599) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..468
                     /product="uncharacterized protein LOC144112434"
                     /calculated_mol_wt=51995
     CDS             1..468
                     /gene="LOC144112434"
                     /coded_by="XM_077645275.1:328..1734"
                     /db_xref="GeneID:144112434"
ORIGIN      
        1 mpqencamql silaltdaaq dvppevklte sceggrhksv qatvwpstqs kavqacvkrs
       61 tasrhsqtkp htvsigiqvg spmidaatqt tmpiqreaie nenttedegg denyddedys
      121 pceefeekqg cvgnstddsg nkiflvqeky lwklfqlcte clapravrfe cegtllkvyg
      181 ecasghllyw cnqdikkqqp mlnvqlcaav lfsgsnptat lrmlasigvp vvsnrtffti
      241 qrsylwpaid rvwkeeqksl lqelqgqqvn lagdgrsdsp gfsakygtyt lmdverhkvl
      301 hfevvqsnda ggscrmeleg lkqglafles anikvavlvt drhtqikcfl rnnktaithe
      361 fdvwtqlaal hynensgrgq artkdgtlrw cvrypkaagg dptacpvkep pthgyvkcll
      421 dkvvamaest qpqerktape srpplsssfp kvpkeklvek rksrfnrq
//
LOCUS       XP_077501402             117 aa            linear   INV 25-NOV-2025
DEFINITION  P2X purinoceptor 7-like [Amblyomma americanum].
ACCESSION   XP_077501402
VERSION     XP_077501402.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645276.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526599) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..117
                     /product="P2X purinoceptor 7-like"
                     /calculated_mol_wt=13452
     Region          <8..112
                     /region_name="P2RX7_C"
                     /note="P2X purinoreceptor 7 intracellular domain;
                     pfam20478"
                     /db_xref="CDD:466627"
     CDS             1..117
                     /gene="LOC144112435"
                     /coded_by="XM_077645276.1:149..502"
                     /db_xref="GeneID:144112435"
ORIGIN      
        1 mtekedclcc qevpeitgkq krarcitttk kfkdvclnka vlevalarqe srasrndrgs
       61 vnkrmryaay rqfvylvwkr lgrhkreilp scvlsavrkk ylsadgsytg fkhpytc
//
LOCUS       XP_077501403             117 aa            linear   INV 25-NOV-2025
DEFINITION  P2X purinoceptor 7-like [Amblyomma americanum].
ACCESSION   XP_077501403
VERSION     XP_077501403.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645277.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526599) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..117
                     /product="P2X purinoceptor 7-like"
                     /calculated_mol_wt=13452
     Region          <8..112
                     /region_name="P2RX7_C"
                     /note="P2X purinoreceptor 7 intracellular domain;
                     pfam20478"
                     /db_xref="CDD:466627"
     CDS             1..117
                     /gene="LOC144112436"
                     /coded_by="XM_077645277.1:149..502"
                     /db_xref="GeneID:144112436"
ORIGIN      
        1 mtekedclcc qevpeitgkq krarcitttk kfkdvclnka vlevalarqe srasrndrgs
       61 vnkrmryaay rqfvylvwkr lgrhkreilp scvlsavrkk ylsadgsytg fkhpytc
//
LOCUS       XP_077501404             468 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112437 [Amblyomma americanum].
ACCESSION   XP_077501404
VERSION     XP_077501404.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645278.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526599) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..468
                     /product="uncharacterized protein LOC144112437"
                     /calculated_mol_wt=51995
     CDS             1..468
                     /gene="LOC144112437"
                     /coded_by="XM_077645278.1:328..1734"
                     /db_xref="GeneID:144112437"
ORIGIN      
        1 mpqencamql silaltdaaq dvppevklte sceggrhksv qatvwpstqs kavqacvkrs
       61 tasrhsqtkp htvsigiqvg spmidaatqt tmpiqreaie nenttedegg denyddedys
      121 pceefeekqg cvgnstddsg nkiflvqeky lwklfqlcte clapravrfe cegtllkvyg
      181 ecasghllyw cnqdikkqqp mlnvqlcaav lfsgsnptat lrmlasigvp vvsnrtffti
      241 qrsylwpaid rvwkeeqksl lqelqgqqvn lagdgrsdsp gfsakygtyt lmdverhkvl
      301 hfevvqsnda ggscrmeleg lkqglafles anikvavlvt drhtqikcfl rnnktaithe
      361 fdvwtqlaal hynensgrgq artkdgtlrw cvrypkaagg dptacpvkep pthgyvkcll
      421 dkvvamaest qpqerktape srpplsssfp kvpkeklvek rksrfnrq
//
LOCUS       XP_077501405             130 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112439 [Amblyomma americanum].
ACCESSION   XP_077501405
VERSION     XP_077501405.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645279.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526610) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..130
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..130
                     /product="uncharacterized protein LOC144112439"
                     /calculated_mol_wt=13672
     CDS             1..130
                     /gene="LOC144112439"
                     /coded_by="XM_077645279.1:21..413"
                     /db_xref="GeneID:144112439"
ORIGIN      
        1 maatcswfsg hgkskktgas efkvekamyd tvkgiqmdav fhkrtaipiw steavvlcss
       61 sgkcsggdsg rqgkgivatq lesscyqgns tystlpekth ammlgasilt ilpilstnea
      121 kdafvqisks
//
LOCUS       XP_077501407             160 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112440 [Amblyomma americanum].
ACCESSION   XP_077501407
VERSION     XP_077501407.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645281.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526610) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..160
                     /product="uncharacterized protein LOC144112440"
                     /calculated_mol_wt=17656
     Region          <1..58
                     /region_name="DDE_3"
                     /note="DDE superfamily endonuclease; cl46863"
                     /db_xref="CDD:481203"
     CDS             1..160
                     /gene="LOC144112440"
                     /coded_by="XM_077645281.1:1..483"
                     /db_xref="GeneID:144112440"
ORIGIN      
        1 mdqgviqnvk vhyrrqllhr mllcadsgkd yvidllsaih ilaraweqvq attvqkcfhh
       61 agfeahdpia heeagtadee adtilnqivp sapfsrqdye didanvcscr egsieeiiae
      121 vqaedhssss decedpvpsa vpdsaarnav alllqyferq
//
LOCUS       XP_077501409             734 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112452 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501409
VERSION     XP_077501409.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645283.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526615) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..734
                     /product="uncharacterized protein LOC144112452 isoform X1"
                     /calculated_mol_wt=66222
     Region          58..108
                     /region_name="CBM_14"
                     /note="Chitin binding Peritrophin-A domain; pfam01607"
                     /db_xref="CDD:426342"
     CDS             1..734
                     /gene="LOC144112452"
                     /coded_by="XM_077645283.1:124..2328"
                     /db_xref="GeneID:144112452"
ORIGIN      
        1 mkitggllll lalasplhck rtkrwdeskg qnqegwvrgt agvdypdyke iprtsfrcsq
       61 qpyegmyadl etqcqvyhvc hngrkesfkc gpgtifnqqi racdywysfe cdeapnwyyl
      121 naevnvespq plqplpggtg gaggvggggr pgpaylpggt ggaaggggap gaggrpgagg
      181 apgaggapga ggypgsggag gypgrpgspg sggypggsgs pgsgrypgsg gapgsggapg
      241 sggypggagg ypsggagrpg gagypgsgag gpgaggypgs gagrpgaggy pgaggygpga
      301 gsrpgaggqy rkpsdgpggp ssggyggsgg pggpggygga ggpagpggpg gsggygpsgp
      361 ggpggpggpg ccggpsgpgg pggpggpggp kgpggpggpg gpggpggpgg pgaaggpggy
      421 pgsggygapg sggagaapgg pgpyiasggp gsgsypgagg vrpgggsrps aggygvgpsg
      481 pgpapstypg ssgaggypgg agagpgasgp gsysstysar ssysssssys sytdsgsgsn
      541 ypgsvgpgvy piagtggaga pgsggdvygs ypksagshfp kkgshefesp sspgpgsagf
      601 pkpgagarrp ggggvgggga ggggaygepl sypgsggydt aplspiqgaq pgggaawqgp
      661 eypspnaggg gsgggggyyt dasggysdgs gypgqdaagq qpgatpqrpr gggrgrrpqg
      721 gyggpmmmgg aggd
//
LOCUS       XP_077501410             734 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112452 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501410
VERSION     XP_077501410.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645284.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526615) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..734
                     /product="uncharacterized protein LOC144112452 isoform X1"
                     /calculated_mol_wt=66222
     Region          58..108
                     /region_name="CBM_14"
                     /note="Chitin binding Peritrophin-A domain; pfam01607"
                     /db_xref="CDD:426342"
     CDS             1..734
                     /gene="LOC144112452"
                     /coded_by="XM_077645284.1:160..2364"
                     /db_xref="GeneID:144112452"
ORIGIN      
        1 mkitggllll lalasplhck rtkrwdeskg qnqegwvrgt agvdypdyke iprtsfrcsq
       61 qpyegmyadl etqcqvyhvc hngrkesfkc gpgtifnqqi racdywysfe cdeapnwyyl
      121 naevnvespq plqplpggtg gaggvggggr pgpaylpggt ggaaggggap gaggrpgagg
      181 apgaggapga ggypgsggag gypgrpgspg sggypggsgs pgsgrypgsg gapgsggapg
      241 sggypggagg ypsggagrpg gagypgsgag gpgaggypgs gagrpgaggy pgaggygpga
      301 gsrpgaggqy rkpsdgpggp ssggyggsgg pggpggygga ggpagpggpg gsggygpsgp
      361 ggpggpggpg ccggpsgpgg pggpggpggp kgpggpggpg gpggpggpgg pgaaggpggy
      421 pgsggygapg sggagaapgg pgpyiasggp gsgsypgagg vrpgggsrps aggygvgpsg
      481 pgpapstypg ssgaggypgg agagpgasgp gsysstysar ssysssssys sytdsgsgsn
      541 ypgsvgpgvy piagtggaga pgsggdvygs ypksagshfp kkgshefesp sspgpgsagf
      601 pkpgagarrp ggggvgggga ggggaygepl sypgsggydt aplspiqgaq pgggaawqgp
      661 eypspnaggg gsgggggyyt dasggysdgs gypgqdaagq qpgatpqrpr gggrgrrpqg
      721 gyggpmmmgg aggd
//
LOCUS       XP_077501411             597 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112452 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501411
VERSION     XP_077501411.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645285.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526615) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..597
                     /product="uncharacterized protein LOC144112452 isoform X2"
                     /calculated_mol_wt=53366
     Region          58..108
                     /region_name="CBM_14"
                     /note="Chitin binding Peritrophin-A domain; pfam01607"
                     /db_xref="CDD:426342"
     CDS             1..597
                     /gene="LOC144112452"
                     /coded_by="XM_077645285.1:123..1916"
                     /db_xref="GeneID:144112452"
ORIGIN      
        1 mkitggllll lalasplhck rtkrwdeskg qnqegwvrgt agvdypdyke iprtsfrcsq
       61 qpyegmyadl etqcqvyhvc hngrkesfkc gpgtifnqqi racdywysfe cdeapnwyyl
      121 naevnvespq plqplpggtg gaggvggggr pgpaylpggt ggaaggggap gaggrpgagg
      181 apgaggapga ggypgsggag gypgrpgspg sggypggsgs pgsgrypgsg gapgsggapg
      241 sggypggagg ypsggagrpg gagypgsgag gpgaggypgs gagrpgaggy pgaggygpga
      301 gsrpgaggqy rkpsdgpggp ssggyggsgg pggpggygga ggpagpggpg gsggygpsgp
      361 ggpggpggpg ccggpsgpgg pggpggpggp kgpggpggpg gpggpggpgg pgaaggpggy
      421 pgsggygapg sggagaapgg pgpyiasggp gsgsypgagg vrpgggsrps aggygvgpsg
      481 pgpapstypg ssgaggypgg agagpgasgp gaqpgggaaw qgpeypspna ggggsggggg
      541 yytdasggys dgsgypgqda agqqpgatpq rprgggrgrr pqggyggpmm mggaggd
//
LOCUS       XP_077501412             114 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112454 [Amblyomma americanum].
ACCESSION   XP_077501412
VERSION     XP_077501412.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645286.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526617) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..114
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..114
                     /product="uncharacterized protein LOC144112454"
                     /calculated_mol_wt=11940
     CDS             1..114
                     /gene="LOC144112454"
                     /coded_by="XM_077645286.1:199..543"
                     /db_xref="GeneID:144112454"
ORIGIN      
        1 mtaacsgker pavpqrllag sssqhqgeal lvpadalcsl skatmltkgk rsprilqale
       61 qlcgrgfpcv rveeptadae eqaalegrce elecgnsrin gklksalsyl qtaa
//
LOCUS       XP_077501413             262 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112455 [Amblyomma americanum].
ACCESSION   XP_077501413
VERSION     XP_077501413.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645287.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526629) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..262
                     /product="uncharacterized protein LOC144112455"
                     /calculated_mol_wt=29043
     Region          21..108
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..262
                     /gene="LOC144112455"
                     /coded_by="XM_077645287.1:151..939"
                     /db_xref="GeneID:144112455"
ORIGIN      
        1 maapmpvscv ssqesradmk rvswshhatm tlirlweenl palracsrna riyasisael
       61 saglhgegpy tskqvrqkid nlhkkyrkik rtgtttgsgg qdwpfywhlh qflgslpmnd
      121 emlieenvev pvvaempeda elvswgrlaf aendatasep ligneatkts rlnttaadvs
      181 nralsssadp gpssispark rqsssavqql lhfhseeskr slkaakknrk lqkkvvellq
      241 esnniqnkmv dmmgdflaek kk
//
LOCUS       XP_077501414             187 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112458 [Amblyomma americanum].
ACCESSION   XP_077501414
VERSION     XP_077501414.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645288.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526631) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="uncharacterized protein LOC144112458"
                     /calculated_mol_wt=21178
     CDS             1..187
                     /gene="LOC144112458"
                     /coded_by="XM_077645288.1:840..1403"
                     /db_xref="GeneID:144112458"
ORIGIN      
        1 mrerrkikar sgaspdelyq pkwahykslm fldtscssqp sfstlhtqee eeeterqceh
       61 wnnesadqem dfpcssqnca epslpaqqig vgtsrpgkkr rqnddeteqr rvllttavet
      121 lqrsqqrlaa ndecdafgsm mahavrqipp gpdrqlamln ahqaivkinl srhsvpveml
      181 nvlnmqe
//
LOCUS       XP_077501415             270 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112459 [Amblyomma americanum].
ACCESSION   XP_077501415
VERSION     XP_077501415.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645289.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526636) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..270
                     /product="uncharacterized protein LOC144112459"
                     /calculated_mol_wt=28945
     CDS             1..270
                     /gene="LOC144112459"
                     /coded_by="XM_077645289.1:258..1070"
                     /db_xref="GeneID:144112459"
ORIGIN      
        1 mesdesgnfr kapkqksspk esdpdaskli gtqkqvnpgq pkprrtrkka tvaagavdls
       61 pgkpsisrrs aqppsprepv alkdgvpkap aptsgktalq knhcvkktag lpgseatgps
      121 tdevkilhrr grtsgtprea sditdggart rstsrrpgsk srsdrgekrh rssrhqsrss
      181 gggrrrprsh rqsragssar sisgkrtskk rrhsrskssk rssgrkrtgg rhhrstsmss
      241 asgaakiypk kiprsrspgs arrkhgasky
//
LOCUS       XP_077501417             189 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112460 [Amblyomma americanum].
ACCESSION   XP_077501417
VERSION     XP_077501417.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645291.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526636) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 28% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..189
                     /product="uncharacterized protein LOC144112460"
                     /calculated_mol_wt=21684
     Region          71..127
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..189
                     /gene="LOC144112460"
                     /coded_by="XM_077645291.1:1..570"
                     /db_xref="GeneID:144112460"
ORIGIN      
        1 mretnatral raaqvyafvs eprvtgrwar rqgarksrrq rgrlgaadeg asfclsdava
       61 qscldkdgkk khtrptfsgh qiyvlektfe qtkylagper aklayalgms esqvkvwfqn
      121 rrtkwrkkha aematakkkh dneaeqlrhe dvsdhdeleh gdakkmrqed pllrhhhrdd
      181 rrpdslasi
//
LOCUS       XP_077501418             294 aa            linear   INV 25-NOV-2025
DEFINITION  ribose-5-phosphate isomerase isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501418
VERSION     XP_077501418.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645292.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526636) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..294
                     /product="ribose-5-phosphate isomerase isoform X1"
                     /calculated_mol_wt=31878
     Region          55..282
                     /region_name="RPI_A"
                     /note="Ribose 5-phosphate isomerase type A (RPI_A)
                     subfamily; RPI catalyzes the reversible conversion of
                     ribose-5-phosphate to ribulose 5-phosphate, the first step
                     of the non-oxidative branch of the pentose phosphate
                     pathway. This reaction leads to the...; cd01398"
                     /db_xref="CDD:238692"
     Site            order(89,118,121..122,131,133,160..161,225..226,235,237,
                     241)
                     /site_type="other"
                     /note="tetramer (dimer of dimers) interface [polypeptide
                     binding]"
                     /db_xref="CDD:238692"
     Site            order(89,91..92,145..146,148,158..161,167,185)
                     /site_type="active"
                     /db_xref="CDD:238692"
     Site            order(134,138,166,173,204..205,207..209,212,261..262,267)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:238692"
     CDS             1..294
                     /gene="Rpi"
                     /coded_by="XM_077645292.1:15..899"
                     /db_xref="GeneID:144112462"
ORIGIN      
        1 mgvlfsdkvv rglgfltnvs cphthvlshs rlafrlsplv trrsisggmd lvekakeaaa
       61 qravdnhvkv ngrsmlhfse nnfivgigsg stvvyavehl arrvkehgwk iscvptsfqa
      121 kelirkhglv mtdleespel nvaidgadei dakltlikgg ggcltqekii ascakefvvv
      181 adyrkdsstl genwkkgipv evipmsyvpv qrkiekllgh kaelrqaakk cgpvvtdngn
      241 fildckftpq kdwsavntal kmipgvvetg lfvgmahkay yglndgsvne kkaa
//
LOCUS       XP_077501419             283 aa            linear   INV 25-NOV-2025
DEFINITION  ribose-5-phosphate isomerase isoform X2 [Amblyomma americanum].
ACCESSION   XP_077501419
VERSION     XP_077501419.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645293.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526636) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..283
                     /product="ribose-5-phosphate isomerase isoform X2"
                     /calculated_mol_wt=30620
     Region          55..271
                     /region_name="RPI_A"
                     /note="Ribose 5-phosphate isomerase type A (RPI_A)
                     subfamily; RPI catalyzes the reversible conversion of
                     ribose-5-phosphate to ribulose 5-phosphate, the first step
                     of the non-oxidative branch of the pentose phosphate
                     pathway. This reaction leads to the...; cd01398"
                     /db_xref="CDD:238692"
     Site            order(78,107,110..111,120,122,149..150,214..215,224,226,
                     230)
                     /site_type="other"
                     /note="tetramer (dimer of dimers) interface [polypeptide
                     binding]"
                     /db_xref="CDD:238692"
     Site            order(78,80..81,134..135,137,147..150,156,174)
                     /site_type="active"
                     /db_xref="CDD:238692"
     Site            order(123,127,155,162,193..194,196..198,201,250..251,256)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:238692"
     CDS             1..283
                     /gene="Rpi"
                     /coded_by="XM_077645293.1:13..864"
                     /db_xref="GeneID:144112462"
ORIGIN      
        1 mgvlfsdkvv rglgfltnvs cphthvlshs rlafrlsplv trrsisggmd lvekakeaaa
       61 qravdnhvkn nfivgigsgs tvvyavehla rrvkehgwki scvptsfqak elirkhglvm
      121 tdleespeln vaidgadeid akltlikggg gcltqekiia scakefvvva dyrkdsstlg
      181 enwkkgipve vipmsyvpvq rkiekllghk aelrqaakkc gpvvtdngnf ildckftpqk
      241 dwsavntalk mipgvvetgl fvgmahkayy glndgsvnek kaa
//
LOCUS       XP_077501420              88 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112463 [Amblyomma americanum].
ACCESSION   XP_077501420
VERSION     XP_077501420.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645294.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526636) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..88
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..88
                     /product="uncharacterized protein LOC144112463"
                     /calculated_mol_wt=9994
     Region          22..>78
                     /region_name="Corazonin"
                     /note="Pro-corazonin; pfam17308"
                     /db_xref="CDD:375119"
     CDS             1..88
                     /gene="LOC144112463"
                     /coded_by="XM_077645294.1:101..367"
                     /db_xref="GeneID:144112463"
ORIGIN      
        1 mshsvavcgv llvclvmsay cqtfqysrgw tngkrrdgai valpsrltae hrvldeflsk
       61 ftprdrfile rlghllrald htdeeqey
//
LOCUS       XP_077501421             218 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112464 [Amblyomma americanum].
ACCESSION   XP_077501421
VERSION     XP_077501421.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645295.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526641) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 27% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..218
                     /product="uncharacterized protein LOC144112464"
                     /calculated_mol_wt=24813
     CDS             1..218
                     /gene="LOC144112464"
                     /coded_by="XM_077645295.1:1..657"
                     /db_xref="GeneID:144112464"
ORIGIN      
        1 mvamsrgawp spthapllia tivattcrar llgfyqrkgl ipsevtglfg lfkpsfghak
       61 rvckilrsel wnqvrllqdl lraacyaqnq ewlagirhrq fmklavqtte vwwkcsinqa
      121 irrmeelrkv kmkaperqdv lvlggaevpe efasvlkegp kfsvpailkp hewlslnrrl
      181 anrteqenqe rclldgidti trsqgwkrqa slipsfll
//
LOCUS       XP_077501422             117 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112465 [Amblyomma americanum].
ACCESSION   XP_077501422
VERSION     XP_077501422.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645296.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526641) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..117
                     /product="uncharacterized protein LOC144112465"
                     /calculated_mol_wt=13382
     CDS             1..117
                     /gene="LOC144112465"
                     /coded_by="XM_077645296.1:116..469"
                     /db_xref="GeneID:144112465"
ORIGIN      
        1 mklavqttev wwkcsinqai rrmeelrkvk mkaperqdvl vlggaevpee fasvlkegpk
       61 fsvpailkph ewlslnrrla nrteqenqer clldgidtit rsqgwkrqas lipsfll
//
LOCUS       XP_077501423             189 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112467 [Amblyomma americanum].
ACCESSION   XP_077501423
VERSION     XP_077501423.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645297.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526643) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 26% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..189
                     /product="uncharacterized protein LOC144112467"
                     /calculated_mol_wt=20868
     CDS             1..189
                     /gene="LOC144112467"
                     /coded_by="XM_077645297.1:1..570"
                     /db_xref="GeneID:144112467"
ORIGIN      
        1 mdadsltrsa tmwsdygdrp esddtvlemy prpnqeallt papellavap reekrrathr
       61 rrrerwasqr dlyaarsrar rlsrlwlllp vltavavvaf icwllfvpll tpsrrrrgga
      121 arrggvadnv ttvgddtaad laallsstmg ddvielmpah fwrnssvlva slmvrsaass
      181 nssqegsfs
//
LOCUS       XP_077501425             170 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112469 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501425
VERSION     XP_077501425.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645299.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526644) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..170
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..170
                     /product="uncharacterized protein LOC144112469 isoform X1"
                     /calculated_mol_wt=17969
     CDS             1..170
                     /gene="LOC144112469"
                     /coded_by="XM_077645299.1:228..740"
                     /db_xref="GeneID:144112469"
ORIGIN      
        1 mrrvlvsttr ststiaaakq ekkavtgirr vfvssiinrt maatcswfsg hgkskktgas
       61 efkvekavyd tvkgipmdav fhkrtaipiw steavvlcss sgkcsggdsg rqgkgivatq
      121 lesscyqgns tystlpekth ammvgasilt ilpilstnea kdafgqisks
//
LOCUS       XP_077501426             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112469 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501426
VERSION     XP_077501426.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645300.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526644) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144112469 isoform X2"
                     /calculated_mol_wt=16586
     CDS             1..158
                     /gene="LOC144112469"
                     /coded_by="XM_077645300.1:388..864"
                     /db_xref="GeneID:144112469"
ORIGIN      
        1 msplsrfgav llcacsayia ehniinrtma atcswfsghg kskktgasef kvekavydtv
       61 kgipmdavfh krtaipiwst eavvlcsssg kcsggdsgrq gkgivatqle sscyqgnsty
      121 stlpektham mvgasiltil pilstneakd afgqisks
//
LOCUS       XP_077501427             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112470 [Amblyomma americanum].
ACCESSION   XP_077501427
VERSION     XP_077501427.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645301.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526645) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144112470"
                     /calculated_mol_wt=13485
     CDS             1..133
                     /gene="LOC144112470"
                     /coded_by="XM_077645301.1:456..857"
                     /db_xref="GeneID:144112470"
ORIGIN      
        1 maaragqvac pvaqgpsprr rerppagsap avsatqampq kkaprkktpk aasppatkna
       61 grhrksasss pplgrpagsg nyagpkfsep pspgalprpp vqwmscgaqq aaapsevcle
      121 ianqlklllk vna
//
LOCUS       XP_077501428            1379 aa            linear   INV 25-NOV-2025
DEFINITION  intraflagellar transport protein Oseg6 [Amblyomma americanum].
ACCESSION   XP_077501428
VERSION     XP_077501428.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645302.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526645) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1379
                     /product="intraflagellar transport protein Oseg6"
                     /calculated_mol_wt=154252
     Region          <44..206
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          99..135
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          143..177
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          184..220
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          228..270
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          276..313
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          315..>374
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          319..355
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          362..408
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          548..604
                     /region_name="WD40_3"
                     /note="WD domain, G-beta repeat; pfam15911"
                     /db_xref="CDD:464937"
     Region          819..1047
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Region          881..904
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(883..884,887..888,890,906,909..910,913..914,
                     916..917,935,938..939,942..943,946)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          905..931
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          934..960
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          1291..1356
                     /region_name="DZR_2"
                     /note="Double zinc ribbon domain; pfam18912"
                     /db_xref="CDD:465906"
     CDS             1..1379
                     /gene="Oseg6"
                     /coded_by="XM_077645302.1:132..4271"
                     /db_xref="GeneID:144112472"
ORIGIN      
        1 mfsrsaqaka akasaaregt pspmatslvy ttagyrayig asmkavftlp ekthgpgpvf
       61 ftwqrssgny lvttgydqtv nvydrhgdrk dqislpgmcs glgydkdgdt lgiitdrsar
      121 lflwdantqq laqvdtglrd vltlllwakt gpflaigtsk gnlliyhhrs crkvpilgkh
      181 akritygaws qqnmlalvge dnvltvsnqe gdtlcqttlk seatlvqfsc mkkderstae
      241 ntvslvlnkk tlllldiydp enpyvlafqe mygkiieykw ygagyillgf tngyfvtist
      301 spkdigqemm qvrchkqsls hiaiclsqnk vascsdniik vydlsdlqdv qsvvtvddvr
      361 kiewldwsdd gqflgaggag gslhvfltkl ptlgnslghr fayltsltev tvdsvtepel
      421 hmtlrvdiep sfialgpfhl vvgmnnhawf yalgdksmvp lhdkeylgtv rsmklngdya
      481 aalfdgklql qlietensdl eeresklfpd saqghvkilc htvtedfviy gtdtgfieff
      541 fledwtainr ykhssgirql hldpsntrlv vlderlqgfl ynpvtdhvid ipnipsaarg
      601 vfwdqhldnr gcfvvfddin ayvymyvsyt vvgalvetir kvsipsgqyp lllhdgsltc
      661 qtqsgktall lvtgtedkgr gtvaqqrqal deylklrrfs dawqvcetln skedwiafgn
      721 salsnldvdl airvfrkigd vgmvwslrel ceiedkslla aylamfkgdf gvaqelflas
      781 sqpaaalqmr rdllhweqal qlakrlaqkq ipfisreyaq qlelagdhas altyyesgit
      841 ndlshtdhdk acraglarts lrcgdirkgv kvakeldsrl llrecaeile smkqhqeaap
      901 lyeqgnyydk aanlyirlkn wskvgellkh vtspkiyaqy akakeadgrf aeaakayeqa
      961 hdienvvriq lntlnnleea vrlvkeskst egarlvakff qklgdsvsai qflvlskcsd
     1021 eafqlaksck kmdayanaig esgspddfhs iaiyyeeern nfeagkfyfk agqykqamkl
     1081 llkattkddd epinlivqaa aaaaddqitr qvieflmget dgipkdfkhl frlymglgqy
     1141 reasktavvi areeqnagny rnahdvllnm yqelrkqqvk vpaemhhnlm llhsyilvkv
     1201 hirrgdhmqa arllervans isrfpahvvp iltsaviecq raglknsafi yastlmrpey
     1261 rsqidtkyrk kveaivrkph reqeeaahsp cpfcscslae mellcshcta nlpfclatgk
     1321 hivkddftqc prcgfpaihs qfcavlasea scpmclepvs adqlqlaqfe pqvhadtee
//
LOCUS       XP_077501429            1001 aa            linear   INV 25-NOV-2025
DEFINITION  coiled-coil domain-containing protein 186-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077501429
VERSION     XP_077501429.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645303.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526645) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1001
                     /product="coiled-coil domain-containing protein 186-like
                     isoform X1"
                     /calculated_mol_wt=111737
     Region          <323..748
                     /region_name="Mplasa_alph_rch"
                     /note="helix-rich Mycoplasma protein; TIGR04523"
                     /db_xref="CDD:275316"
     CDS             1..1001
                     /gene="LOC144112473"
                     /coded_by="XM_077645303.1:181..3186"
                     /db_xref="GeneID:144112473"
ORIGIN      
        1 messildgta dvsteaarrg pesssiacaa qllpseheph lveepphpqg vsdqaasecv
       61 vitssesclv slseecdapg ckqesrssls lqncqlsgag pssaahcpsn aslgdiavaa
      121 plpevaalsa passndgfhl sdraksadya dspvrenaps tyralsarsa dmdcpavlrv
      181 sssrsspevr lfvdhcasse sdsssrhcer ldkkseshnl cryssdshlp rcvrtptels
      241 scsqesddsd allselesel egvdgsessk lawglhngsq avalrckrca lsydiverle
      301 qsnnqlqqql tvlnektsar eaesqrllaq traellskne klckqleavt kekdsmvvky
      361 atsekevila rkaledtekk yrevvkerdq hfsrvrvlnn erarlcssld tkladmaklq
      421 keqerlkeel sardikikwa qnklktevda hretqakleq aqlrlrdlre eaeqvrrdcq
      481 emirryqeae eiksvsldhq lkeklselee qkmekesqee vyhvmkqeld slkkkhkmsi
      541 denngltiki hnlekerldy eqtlsklkek lnalkqeivd lngkldekrn velqlerere
      601 kvvasqqeve rlrqsaaemq tdmeachlke gellefterl ttksvqlqse hsllelkaqn
      661 leeqnsklmq evaqlkkqnq elassveseq kqrqqetqll arklaektkt iekltmqved
      721 aeneqrvmkr rhissikels relqltkkrl elqqdsqgsp rqvgapeplg lgsrtsstas
      781 letlgsgpiq aatsnhptvt fvnagrstvp pkesrlhaaa vtvlngsarn cigspgasga
      841 sscgspehas tapaqleldk qmlverivrl qknlarrndk iefleehikq lllemkkksk
      901 iiqnyiiree agalvptsmd cnkaeiskkg gimasvynsh avdatmtlel sleinqklqa
      961 vledtllkni tlkenintlg eeiarlskdy rnldatqcrr k
//
LOCUS       XP_077501430            1001 aa            linear   INV 25-NOV-2025
DEFINITION  coiled-coil domain-containing protein 186-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077501430
VERSION     XP_077501430.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645304.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526645) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1001
                     /product="coiled-coil domain-containing protein 186-like
                     isoform X1"
                     /calculated_mol_wt=111737
     Region          <323..748
                     /region_name="Mplasa_alph_rch"
                     /note="helix-rich Mycoplasma protein; TIGR04523"
                     /db_xref="CDD:275316"
     CDS             1..1001
                     /gene="LOC144112473"
                     /coded_by="XM_077645304.1:199..3204"
                     /db_xref="GeneID:144112473"
ORIGIN      
        1 messildgta dvsteaarrg pesssiacaa qllpseheph lveepphpqg vsdqaasecv
       61 vitssesclv slseecdapg ckqesrssls lqncqlsgag pssaahcpsn aslgdiavaa
      121 plpevaalsa passndgfhl sdraksadya dspvrenaps tyralsarsa dmdcpavlrv
      181 sssrsspevr lfvdhcasse sdsssrhcer ldkkseshnl cryssdshlp rcvrtptels
      241 scsqesddsd allselesel egvdgsessk lawglhngsq avalrckrca lsydiverle
      301 qsnnqlqqql tvlnektsar eaesqrllaq traellskne klckqleavt kekdsmvvky
      361 atsekevila rkaledtekk yrevvkerdq hfsrvrvlnn erarlcssld tkladmaklq
      421 keqerlkeel sardikikwa qnklktevda hretqakleq aqlrlrdlre eaeqvrrdcq
      481 emirryqeae eiksvsldhq lkeklselee qkmekesqee vyhvmkqeld slkkkhkmsi
      541 denngltiki hnlekerldy eqtlsklkek lnalkqeivd lngkldekrn velqlerere
      601 kvvasqqeve rlrqsaaemq tdmeachlke gellefterl ttksvqlqse hsllelkaqn
      661 leeqnsklmq evaqlkkqnq elassveseq kqrqqetqll arklaektkt iekltmqved
      721 aeneqrvmkr rhissikels relqltkkrl elqqdsqgsp rqvgapeplg lgsrtsstas
      781 letlgsgpiq aatsnhptvt fvnagrstvp pkesrlhaaa vtvlngsarn cigspgasga
      841 sscgspehas tapaqleldk qmlverivrl qknlarrndk iefleehikq lllemkkksk
      901 iiqnyiiree agalvptsmd cnkaeiskkg gimasvynsh avdatmtlel sleinqklqa
      961 vledtllkni tlkenintlg eeiarlskdy rnldatqcrr k
//
LOCUS       XP_077501431             979 aa            linear   INV 25-NOV-2025
DEFINITION  coiled-coil domain-containing protein 186-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077501431
VERSION     XP_077501431.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645305.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526645) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..979
                     /product="coiled-coil domain-containing protein 186-like
                     isoform X2"
                     /calculated_mol_wt=109448
     Region          <315..>784
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..979
                     /gene="LOC144112473"
                     /coded_by="XM_077645305.1:181..3120"
                     /db_xref="GeneID:144112473"
ORIGIN      
        1 messildgta dvsteaarrg pesssiacaa qllpseheph lveepphpqg vsdqaasecv
       61 vitssesclv slseecdapg ckqesrssls lqncqlsgag pssaahcpsn aslgdiavaa
      121 plpevaalsa passndgfhl sdraksadya dspvrenaps tyralsarsa dmdcpavlrv
      181 sssrsspevr lfvdhcasse sdsssrhcer ldkkseshnl cryssdshlp rcvrtptels
      241 scsqesddsd allselesel egvdgsessk lawglhngsq avalrckrca lsydiverle
      301 qsnnqlqqql tvlnektsar eaesqrllaq traellskne klckqleavt kekdsmvvky
      361 atsekevila rkaledtekk yrevvkerdq hfsrvrvlnn erarlcssld tkladmaklq
      421 keqerlkeel sardikikwa qnklktevda hretqakleq aqlrlrdlre eaeqvrrdcq
      481 emirryqeae eiksvsldhq lkeklselee qkmekesqee vyhvmkqeld slkkkhkmsi
      541 denngltiki hnlekerldy eqtlsklkek lnalkqeivd lngkldekrn velqlerere
      601 kvvasqqeve rlrqsaaemq tdmeachlke gellefterl ttksvqlqse hsllelkaqn
      661 leeqnsklmq evaqlkkqnq elassveseq kqrqqetqll arklaektkt iekltmqved
      721 aeneqrvmkr rhissikels relqltkkrl elqqdsqgsp rqvgapeplg lgsrtsstas
      781 letlgsgpiq aatsnhptvt fvnagrstvp gspgasgass cgspehasta paqleldkqm
      841 lverivrlqk nlarrndkie fleehikqll lemkkkskii qnyiireeag alvptsmdcn
      901 kaeiskkggi masvynshav datmtlelsl einqklqavl edtllknitl kenintlgee
      961 iarlskdyrn ldatqcrrk
//
LOCUS       XP_077501432             203 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112474 [Amblyomma americanum].
ACCESSION   XP_077501432
VERSION     XP_077501432.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645306.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526645) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..203
                     /product="uncharacterized protein LOC144112474"
                     /calculated_mol_wt=21884
     CDS             1..203
                     /gene="LOC144112474"
                     /coded_by="XM_077645306.1:162..773"
                     /db_xref="GeneID:144112474"
ORIGIN      
        1 mnaaasllal cllseavhga pkpavealva aravlssles apvrdvvlqd rvfrvglgsf
       61 sgeaalsngt lrsvaslrai nltpsegsli ranlllsrls irynanvtfg arteaaemdv
      121 evdslelvve lshdgqldlq svealemdem rvnfrglrvf laqsellsll frgafrfdie
      181 devvavfeel lrelvdaynv nnl
//
LOCUS       XP_077501433             108 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112475 [Amblyomma americanum].
ACCESSION   XP_077501433
VERSION     XP_077501433.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645307.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526647) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..108
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..108
                     /product="uncharacterized protein LOC144112475"
                     /calculated_mol_wt=11710
     CDS             1..108
                     /gene="LOC144112475"
                     /coded_by="XM_077645307.1:40..366"
                     /db_xref="GeneID:144112475"
ORIGIN      
        1 mrvsyafqlf gdtvlnglrl ykteleasgg slepvltffg mirdlieimt srfpakalrp
       61 gsvaegqlls flayltewel hagvqggfls astavglrvt ellvltrk
//
LOCUS       XP_077501434             212 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112478 [Amblyomma americanum].
ACCESSION   XP_077501434
VERSION     XP_077501434.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645308.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526658) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..212
                     /product="uncharacterized protein LOC144112478"
                     /calculated_mol_wt=22143
     CDS             1..212
                     /gene="LOC144112478"
                     /coded_by="XM_077645308.1:291..929"
                     /db_xref="GeneID:144112478"
ORIGIN      
        1 mlgqpkqksk eedarkkrdr hktgggsaqc tvsaqseqvi avashimtrv gnqtdsdggi
       61 dlppvaslpv irllqpvvdg agneefhyae ddwepplvde pqspaaavgk sgatpeaieq
      121 esnlffpavq padasstaaa gctsatvasg aaaspttaea vatgdagrap rgrmallers
      181 ladendyraa llreehglrl qlmredhnsh tl
//
LOCUS       XP_077501435             153 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112479 [Amblyomma americanum].
ACCESSION   XP_077501435
VERSION     XP_077501435.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645309.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526658) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..153
                     /product="uncharacterized protein LOC144112479"
                     /calculated_mol_wt=16824
     CDS             1..153
                     /gene="LOC144112479"
                     /coded_by="XM_077645309.1:48..509"
                     /db_xref="GeneID:144112479"
ORIGIN      
        1 mmmflkdqvd igstfsnshv qdsetaesvl esiysetedg aeppedgafs plvpsrsttp
       61 ephtpngssq astaastlaa prsgrgkkrk ersrwqddie kvdalitedk dqasvygqlv
      121 ahklracpkh akrdmemdll qfiakytfhd npe
//
LOCUS       XP_077501436             256 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112480 [Amblyomma americanum].
ACCESSION   XP_077501436
VERSION     XP_077501436.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645310.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526658) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..256
                     /product="uncharacterized protein LOC144112480"
                     /calculated_mol_wt=28504
     Region          183..>232
                     /region_name="BEN"
                     /note="The BEN domain is found in diverse animal proteins;
                     cl11158"
                     /db_xref="CDD:472128"
     CDS             1..256
                     /gene="LOC144112480"
                     /coded_by="XM_077645310.1:83..853"
                     /db_xref="GeneID:144112480"
ORIGIN      
        1 mdhilikwtq eekwdvypir amvntavgfr lltepgaidd vrgtielftw kdseppapae
       61 vldigktrvl etkrarlasa glrdegdvmt eslecspnee fcsqcenmsr eldvvkrrne
      121 dleaahesqk vlkklkkmld dmksidaisa vrpcpqidig ggvlieetal eklrsscpga
      181 pakfarglmr qlftadelrg kslfgrksns hpdaslreal dpvrvnaiig ftirefkadp
      241 irlksslssl lsreik
//
LOCUS       XP_077501437             572 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112484, partial [Amblyomma
            americanum].
ACCESSION   XP_077501437
VERSION     XP_077501437.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645311.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526667) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         <1..572
                     /product="uncharacterized protein LOC144112484"
     Region          43..153
                     /region_name="PAS_11"
                     /note="PAS domain; pfam14598"
                     /db_xref="CDD:464214"
     CDS             1..572
                     /gene="LOC144112484"
                     /coded_by="XM_077645311.1:<1..1719"
                     /db_xref="GeneID:144112484"
ORIGIN      
        1 vvlvqghfvs ylplcsrnep vflatctpva mpetrecvvq gstsvftsih smdmkflhld
       61 rngefhlgyq ksslqsiswy elvhwehlre aqskhrlitq seqerscill vrlqtsrscw
      121 iwvhavlqvk dgsdssqqpv ivctnqvlse reaavmrans wlyqyyslhs kmhyglayea
      181 trlpayyptp vmpypppppp ppprevpqap lphspyplph hlngapvpqh phhpgvlqyg
      241 gpshhsglpy snvsasvaaa aaavgtpsis spasspdhrk wtsptssstg lkrpaspgpg
      301 ssassagnvs ggssassspt lpkaprpsyg aftelppllp sgyyggpgqv laatapvpaa
      361 fraptklpdd rladveqslt ptntedvsql isgggpgylr ptymslddpe vakaarsvaa
      421 asyptalepp ayyprylels ltleladgke pyaaaaaaaa aaaaaqssad lregflyhrg
      481 afapprlsap cgtgseeeav aaldasaaah aaavaaaahh hhphhywhrq vggggppglg
      541 gpgsaidsta sclpqpqpgh rgiaeepasv es
//
LOCUS       XP_077501438             128 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112486 [Amblyomma americanum].
ACCESSION   XP_077501438
VERSION     XP_077501438.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645312.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526667) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..128
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..128
                     /product="uncharacterized protein LOC144112486"
                     /calculated_mol_wt=14278
     CDS             1..128
                     /gene="LOC144112486"
                     /coded_by="XM_077645312.1:105..491"
                     /db_xref="GeneID:144112486"
ORIGIN      
        1 mvrlllwtsa mislliliqq itaydadegm mqrpieltpp phlftkkgsn mattpktkrk
       61 lgqycsqnhp cdkglccvrr rrfggsscqa lagryrrcsd sptkgdiyhr ycpcaigiya
      121 cengyclp
//
LOCUS       XP_077501439             211 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112487, partial [Amblyomma
            americanum].
ACCESSION   XP_077501439
VERSION     XP_077501439.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645313.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526681) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on the carboxy end.
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..>211
                     /product="uncharacterized protein LOC144112487"
     CDS             1..211
                     /gene="LOC144112487"
                     /coded_by="XM_077645313.1:1129..>1761"
                     /db_xref="GeneID:144112487"
ORIGIN      
        1 maawcsssrl rasktserlv gpgpglssts pesssissds kskakassga kygrsgpddt
       61 addcsgihga eesgkcsvgd gqgsrprrvp aesqydstwk antgvnivqe svscpaeltl
      121 crrlntvasg hviddvvdgg gfqakassga kygrsgpddt addcsgihga eesgkcsvgd
      181 gqgsrprrvp aesqydstwk antgvnivqe s
//
LOCUS       XP_077501440             494 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077501440
VERSION     XP_077501440.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645314.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526696) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..494
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=55186
     Region          81..494
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..494
                     /gene="LOC144112488"
                     /coded_by="XM_077645314.1:548..2032"
                     /db_xref="GeneID:144112488"
ORIGIN      
        1 mavagvlllf falaavgtay dvarsyrssh kkpsssrsvd ttmasneglv nggslgdggs
       61 tfsrsvlafs wlsngskifn tsgkkesiqv ihglrfysma wiilghtfsf skqwityrnm
      121 ndllvihtdi itqglangtv tvdtfffisg llvvyvtlkd mastwgvnrw llfyvhrywr
      181 mtplmmaaia fcaellpycg dgprwkesva tydgtcqrnw winamylqnf ihttdmclnh
      241 twysavdyqf yfiaplilma lqrksklglf ligllllast sftavytaik dypaapymsd
      301 iisleklndf mgnvyikpyf rigpfligma agyvlyrtqg sciirkryvw lgwttcafmm
      361 lgvlyamwpa ntgesvpsva waavyggfar siwamglawi ifasvagygg vvtkilscra
      421 lvplsrltys ayiihpvlmv ifygsreesf dyspyllmyf avgnlvltym aslvlslvfe
      481 apvlalekvl lgrk
//
LOCUS       XP_077501441             264 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112489 [Amblyomma americanum].
ACCESSION   XP_077501441
VERSION     XP_077501441.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645315.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526696) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..264
                     /product="uncharacterized protein LOC144112489"
                     /calculated_mol_wt=29937
     CDS             1..264
                     /gene="LOC144112489"
                     /coded_by="XM_077645315.1:113..907"
                     /db_xref="GeneID:144112489"
ORIGIN      
        1 mkavcvivaa isagcvwags ptdqekvlht lfanigpdnl sladskfnir eagapnnstl
       61 yrfhlkngkl rnlhtklmpk fdkefcrvsv dkrldtlmvk cdfdlkdmva tydgflsyga
      121 nvvqnftvra aitehsynnn pallcvsthg eagcfadkck lhdyfvfita felnttttps
      181 fynftsnpkf qnfkrnqsla revwtdfnvn mytkirfiii nalnstyqme ipqrilklgv
      241 ldkkkfrepg tkrnarkkfl wslg
//
LOCUS       XP_077501442             264 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112489 [Amblyomma americanum].
ACCESSION   XP_077501442
VERSION     XP_077501442.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645316.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526696) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..264
                     /product="uncharacterized protein LOC144112489"
                     /calculated_mol_wt=29937
     CDS             1..264
                     /gene="LOC144112489"
                     /coded_by="XM_077645316.1:134..928"
                     /db_xref="GeneID:144112489"
ORIGIN      
        1 mkavcvivaa isagcvwags ptdqekvlht lfanigpdnl sladskfnir eagapnnstl
       61 yrfhlkngkl rnlhtklmpk fdkefcrvsv dkrldtlmvk cdfdlkdmva tydgflsyga
      121 nvvqnftvra aitehsynnn pallcvsthg eagcfadkck lhdyfvfita felnttttps
      181 fynftsnpkf qnfkrnqsla revwtdfnvn mytkirfiii nalnstyqme ipqrilklgv
      241 ldkkkfrepg tkrnarkkfl wslg
//
LOCUS       XP_077501443             593 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112492 [Amblyomma americanum].
ACCESSION   XP_077501443
VERSION     XP_077501443.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645317.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526698) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 50% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..593
                     /product="uncharacterized protein LOC144112492"
                     /calculated_mol_wt=66053
     Region          359..>527
                     /region_name="EzrA"
                     /note="Septation ring formation regulator, EzrA;
                     pfam06160"
                     /db_xref="CDD:428797"
     CDS             1..593
                     /gene="LOC144112492"
                     /coded_by="XM_077645317.1:1..1782"
                     /db_xref="GeneID:144112492"
ORIGIN      
        1 mftaillfqm ltcwlpssac pssdyqpstt aftlsgakkw nppwlepppv aansnpariq
       61 hfawqrtasp tspvtrpdra llkqsgcash lgpdtvrctm ftaillfqml tcwlpssacp
      121 ssdyqpstta ftlsgakkwn ppwlepppva ansnpariqh fawqrtaspt spvtrpdral
      181 lkqsgcashl gpdtvrctmf taillfqlal glilhgtasg iepteiawvt spvkgtcfha
      241 cdsltsaefl rphypgaatc ehlsthdllg apphewatyt lprncigsgl ggttsmrcan
      301 pfvfivqvsr rfgkcfrssd rflvvlpcpq lclevfdvav flckllllsg gvetnpgpem
      361 aqiakqlkdi aedikeikek rltdienkld aitlletkvq scqteisrmn hvieslekki
      421 ddlenrsrrs nlivyglped eeestetleq avnksiiqdt lkldpvafer ihrlgrpqan
      481 knrpvifkll darnksailk qgfklkdtky aigedfspri reirkklwns akenrqkgdk
      541 vslafdklfi nscafvwdhk tndkvplkkn eeqeetvekr sppttrgrar lkh
//
LOCUS       XP_077501445             117 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112493 [Amblyomma americanum].
ACCESSION   XP_077501445
VERSION     XP_077501445.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645319.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526716) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..117
                     /product="uncharacterized protein LOC144112493"
                     /calculated_mol_wt=12825
     CDS             1..117
                     /gene="LOC144112493"
                     /coded_by="XM_077645319.1:445..798"
                     /db_xref="GeneID:144112493"
ORIGIN      
        1 mqyftrelpf psgqekqlyy avpvasaqev taegivatql esscyqgnst ystlpekmha
       61 mmlgasilti lpilstneak dafvqiaesq qppwkattew lrscwgretg mvvgkfg
//
LOCUS       XP_077501447             548 aa            linear   INV 25-NOV-2025
DEFINITION  prolyl 4-hydroxylase subunit alpha-1-like [Amblyomma americanum].
ACCESSION   XP_077501447
VERSION     XP_077501447.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645321.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526749) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..548
                     /product="prolyl 4-hydroxylase subunit alpha-1-like"
                     /calculated_mol_wt=62220
     Region          38..170
                     /region_name="P4Ha_N"
                     /note="Prolyl 4-Hydroxylase alpha-subunit, N-terminal
                     region; pfam08336"
                     /db_xref="CDD:462433"
     Region          347..526
                     /region_name="P4Hc"
                     /note="Prolyl 4-hydroxylase alpha subunit homologues;
                     smart00702"
                     /db_xref="CDD:214780"
     CDS             1..548
                     /gene="LOC144112494"
                     /coded_by="XM_077645321.1:1840..3486"
                     /db_xref="GeneID:144112494"
ORIGIN      
        1 mwattrrhcg acvlvavasl avlppyssgq lqrdfyssvs nllslvqyeq evrlmllaya
       61 erlkalqssi isfvearqpy hdlrtqsevs dylkhpvhaf hftkrmivdl gfieaqiqql
      121 hasdplqnlt amrvnrllpw dedfngiaas lvrlqdtyll dmndlvaghl ttrdgpgkst
      181 vpgrvpltar dcyfmgqvav hngfydrave wveravdkts qekppsiske elspyldali
      241 kkhddvldsm gkagqhwqty gvpvsqrnkk alqfrtklfd ekltsaqeth nykrlcrgeq
      301 lrtpkmdsql rcryfhgrdg ffrlqpvkie easlspyiii fhdiirdrni ndllafatpr
      361 llrsthygeh gmetslirts snawlgdeda pvalrlnrfl qsllglgtky skgeaehyql
      421 anygvggqyv ahhdflaeiy kdpnrkmddf ervsgdriat lmfylsdvee ggatvfphlg
      481 vrltpkkgna afwwnlnsdg egeqltkhgg cpvlygskwi ankwfrsnsn vfrlpcarnr
      541 seplaplv
//
LOCUS       XP_077501448             338 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112495 [Amblyomma americanum].
ACCESSION   XP_077501448
VERSION     XP_077501448.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645322.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526751) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..338
                     /product="uncharacterized protein LOC144112495"
                     /calculated_mol_wt=37188
     CDS             1..338
                     /gene="LOC144112495"
                     /coded_by="XM_077645322.1:197..1213"
                     /db_xref="GeneID:144112495"
ORIGIN      
        1 maacytdasp taptaaqarl lakksrkcyt aqedlcllre vaatrpfgdd ikwmtvianv
       61 keaisreltm rgvkdridll igywrqqdar nlkksgteeq ysekeqilqd lsdyarainy
      121 epriiprsgg svqnrkrqaa tdrttsvkrs vmdtragstt lvqaaegdcs asvaapsqsc
      181 vlgeiaepes pavrlllsti gqnppdtppp adtaaygeae qpetdgregs pstgglqqqq
      241 qqqqqqhhdp agqshqqtsr sargphqssa grqllpvttt girglqnlgl qlltmreane
      301 yelrqreiqn ekekiaiekq kadnetrria leerkldl
//
LOCUS       XP_077501449             354 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112496 [Amblyomma americanum].
ACCESSION   XP_077501449
VERSION     XP_077501449.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645323.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526751) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..354
                     /product="uncharacterized protein LOC144112496"
                     /calculated_mol_wt=40090
     Region          172..322
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..354
                     /gene="LOC144112496"
                     /coded_by="XM_077645323.1:58..1122"
                     /db_xref="GeneID:144112496"
ORIGIN      
        1 mpksilqdpl llsrlkwsei edllclevfg etrrdplsvr glinidamds gvfrtyfrfe
       61 kddlrklqsa lripemvltp qkvsvpgdea lcitlrrlay pnrlcdledl fgrhssvlss
      121 ltnevlrrvd ecffhllddf nnqkwlnlnt lekfsqaiha kgapltncwa fidgtaraic
      181 rpsrdqklyf sghkrfhalk yqsimcpngi icqldgsypg skhdagnfgi skvysklekl
      241 vqgqsyciyg dpayplrpll lkpyggvslt peqcefnkam sgvrqavewg fgkiaglfaf
      301 vdfkknqklr lqnlpriyka salfanchtc lygaqvshyf gvdppelety ltsl
//
LOCUS       XP_077501451             215 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112502 [Amblyomma americanum].
ACCESSION   XP_077501451
VERSION     XP_077501451.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645325.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526771) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..215
                     /product="uncharacterized protein LOC144112502"
                     /calculated_mol_wt=21957
     CDS             1..215
                     /gene="LOC144112502"
                     /coded_by="XM_077645325.1:257..904"
                     /db_xref="GeneID:144112502"
ORIGIN      
        1 maawcsssrl rasktserlv gpgpglssts pesssissds kskakassga kygrsgpddt
       61 addcsgihga eesgkcsvgd gqgsrprrvp aesqydstwk antgvnivqe svscpaeltl
      121 crrlntvasg hviddvvdgg gfqakassga kygrsgpddt addcsgihga eesgkcsvgd
      181 gqgsrprrvp aesqydstwk antgvnivqe svscf
//
LOCUS       XP_077501452             119 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112504, partial [Amblyomma
            americanum].
ACCESSION   XP_077501452
VERSION     XP_077501452.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645326.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526795) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: incomplete on the carboxy end.
FEATURES             Location/Qualifiers
     source          1..119
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..>119
                     /product="uncharacterized protein LOC144112504"
     Region          80..>119
                     /region_name="RT_RNaseH_2"
                     /note="RNase H-like domain found in reverse transcriptase;
                     pfam17919"
                     /db_xref="CDD:465567"
     CDS             1..119
                     /gene="LOC144112504"
                     /coded_by="XM_077645326.1:1..>358"
                     /db_xref="GeneID:144112504"
ORIGIN      
        1 mkcsfgqrqi kvlshlvdgs garpdpykvr avrefpspcs ssdvrcflgl ssyfrrftpn
       61 fadttrsltt ilkkdvpfpw gfdqarpftt lfaaltappv tahfrpsapt elrsdasgh
//
LOCUS       XP_077501454             106 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112508, partial [Amblyomma
            americanum].
ACCESSION   XP_077501454
VERSION     XP_077501454.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645328.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526804) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on the carboxy end.
FEATURES             Location/Qualifiers
     source          1..106
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..>106
                     /product="uncharacterized protein LOC144112508"
     Region          <91..101
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..106
                     /gene="LOC144112508"
                     /coded_by="XM_077645328.1:513..>831"
                     /db_xref="GeneID:144112508"
ORIGIN      
        1 mtragtggnn gaadggatat ggvanggvag atassrrrlg krlrakaaql aretlgrqll
       61 qglvarsllk garartaalt gaplpwlall mhhpiqmkpa dsenrn
//
LOCUS       XP_077501455             675 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 2-like, partial [Amblyomma
            americanum].
ACCESSION   XP_077501455
VERSION     XP_077501455.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645329.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526815) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: incomplete on the carboxy end.
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..>675
                     /product="endothelin-converting enzyme 2-like"
     Region          143..669
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..675
                     /gene="LOC144112517"
                     /coded_by="XM_077645329.1:142..>2166"
                     /db_xref="GeneID:144112517"
ORIGIN      
        1 msqskreank rssmasvvds ttpkqpepgv pnliganmlm sqtfspapts sktlmspvql
       61 kvetppmsvs eeeeymtegp gwlrmlaiav gvlgttvilg vltflvfsap qgtvfttppp
      121 slcttfgcie hayylnksml rsvspcdnfy qfvcgswtap rrrqtsvgqn lhesvagaiq
      181 aianlhppes rtkgtrkafd myiscveahd ignvlgnlnd lkrfmdqrgl aisaedtgad
      241 aldqlvdlav nwnlnfifem aphkpqgqte wmlviypgsl iehftvseqt gaafgeylrh
      301 hlargfgnat dviaafpafr kqveqatavl neplnstkyp wlrftfgeie ehapnidsah
      361 wtttvnrhfa aysvrfrnds ivfvtdrrvl qalnslmrag rpafrlpvta yiawkfvelf
      421 gwvlddkise irfgetdkql lqearrvaca sfvertfafa msarylfdsv egitrglvss
      481 litsiqdaml dftdnsdwmd pktkgdfklr vsdiertiwi tgisaypgkl talyshfpvm
      541 ihnfvvnwie snralsrrlv slladessat sesdrrtaya eyransdpsq slylyhlnsm
      601 impmsllfkp myvpegtqam nyagfgalys qlqiklldtv giqfnenrql aswmtaspma
      661 rydakvrcff sresq
//
LOCUS       XP_077501456             603 aa            linear   INV 25-NOV-2025
DEFINITION  glycogen binding subunit 76A [Amblyomma americanum].
ACCESSION   XP_077501456
VERSION     XP_077501456.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645330.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526835) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..603
                     /product="glycogen binding subunit 76A"
                     /calculated_mol_wt=64704
     Region          482..582
                     /region_name="CBM_21"
                     /note="Carbohydrate/starch-binding module (family 21);
                     pfam03370"
                     /db_xref="CDD:427265"
     CDS             1..603
                     /gene="Gbs-76A"
                     /coded_by="XM_077645330.1:312..2123"
                     /db_xref="GeneID:144112520"
ORIGIN      
        1 mtpsewqssc aiglascrlr aeslarqlyc kpfpsmddrp tavvrcppcr qqsasstsvn
       61 gstgswpspp plpapqdasn flvrtgraly drlfsliqdq atdcvvqyce qgsnaddpss
      121 svdesadape qgvlqkqlpp vdscrredvv veeprsaasp erplqsaatg avsrreeaks
      181 dsdelktedq prrypaerdv vraalahrap ddsfpfpivq elvrpgvlvq nesdsscdls
      241 ddafhdaadg laeefrdgsg ssetsneekn aaaaattdvi ctvaaatpad apsaaamptp
      301 dsvsspssss tqetspsrpr ssrfakhrfa eapprkmtpr apelpspehp psdessssss
      361 etlspfadaq ppppatppkr dsaadsdded ggrgvlvrst slksgksppg tpfkkkivrf
      421 adalgldlaa vrtivseelp mvpasafshl qlapvvrspp pctlelkalf aqpgasptfl
      481 srltsqrvcl esvvssgcal havirvlnva feklvvlrys ldnwrsfcdl ravylpgssd
      541 gisdrfgvdv yltpqgdaqv qmcvryvvge qefwdnnega nycfefrdsp kdepevtplm
      601 nyl
//
LOCUS       XP_077501457             179 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112521 [Amblyomma americanum].
ACCESSION   XP_077501457
VERSION     XP_077501457.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645331.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526835) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..179
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..179
                     /product="uncharacterized protein LOC144112521"
                     /calculated_mol_wt=18382
     Region          102..153
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(102,105,124,127,130,133,151)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:259829"
     CDS             1..179
                     /gene="LOC144112521"
                     /coded_by="XM_077645331.1:138..677"
                     /db_xref="GeneID:144112521"
ORIGIN      
        1 measrcssgc sselasasra agqgprpacs acgrpatrpl gqdlycaacy sgalllspgl
       61 apsrcspaps acsssgswsl vtactpnqga gsrqgyggge acarchetvy faerqvgtsg
      121 rvyhvrcfrc hacgqrlasg syrsaggely cepchrrhlg prgygyglgs alrcfpgrl
//
LOCUS       XP_077501458             273 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112522 [Amblyomma americanum].
ACCESSION   XP_077501458
VERSION     XP_077501458.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645332.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526842) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..273
                     /product="uncharacterized protein LOC144112522"
                     /calculated_mol_wt=29535
     Region          <71..105
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..273
                     /gene="LOC144112522"
                     /coded_by="XM_077645332.1:1..822"
                     /db_xref="GeneID:144112522"
ORIGIN      
        1 mggcaspsqf detsgkwpay hfrleaffeg ndvtgdkkkr alliavltth tvdllngrch
       61 pnravpvmaa arilrwalll saydcvikhq pgkdnvlvdv lsrlptsats qpetldetgd
      121 gdtpakllmg yelrsrldna vappgplapq spvdrhvpsv lrpgepdwvk nfgrgepwip
      181 aritssdgtr ivnadgpqge nirrhtdqik prvgehgrdd gtgdherqdq takpqvvgts
      241 gpgpgirkaa pstptpalrr sgrtrkppdr yvp
//
LOCUS       XP_077501459             350 aa            linear   INV 25-NOV-2025
DEFINITION  MAM and LDL-receptor class A domain-containing protein 1-like,
            partial [Amblyomma americanum].
ACCESSION   XP_077501459
VERSION     XP_077501459.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645333.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526842) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on both ends.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         <1..>350
                     /product="MAM and LDL-receptor class A domain-containing
                     protein 1-like"
     Region          <1..110
                     /region_name="MAM"
                     /note="Meprin, A5 protein, and protein tyrosine
                     phosphatase Mu (MAM) domain. MAM is an extracellular
                     domain which mediates protein-protein interactions and is
                     found in a diverse set of proteins, many of which are
                     known to function in cell adhesion. Members...; cd06263"
                     /db_xref="CDD:99706"
     Region          120..152
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(126,134,145..146)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(138,141,145,151..152)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            148..152
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          178..347
                     /region_name="MAM"
                     /note="MAM domain, meprin/A5/mu; pfam00629"
                     /db_xref="CDD:459878"
     CDS             1..350
                     /gene="LOC144112523"
                     /coded_by="XM_077645333.1:<1..>1053"
                     /codon_start=3
                     /db_xref="GeneID:144112523"
ORIGIN      
        1 hflyfgvrpg gasapvnvss pwfrqaalqr ctlvfdmhas nmadgalvvf lsnpnhtrev
       61 hkvpgnslgl wqrfkaplgr qeppfivtie vlpgksapgh lalddirlen carqprrmgt
      121 cletqfrcan stcidrqevc dirvncadge dekqacdqvp rgarcsleee dgesdssdpt
      181 icgwrndvrt sngqwrrvta enatsrlgpq hdhttdspkg hyacatgkqf gheavlespe
      241 fpklpyyhmn htsayfnsch vrlhyyahrs vhpygltlya verdprhasg lrhelwsasg
      301 kddavwlrvv ralpalrhpf ilrlfaqhgq gwkrciavdd itlgpecfgi
//
LOCUS       XP_077501460             508 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112525 [Amblyomma americanum].
ACCESSION   XP_077501460
VERSION     XP_077501460.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645334.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526860) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..508
                     /product="uncharacterized protein LOC144112525"
                     /calculated_mol_wt=57685
     CDS             1..508
                     /gene="LOC144112525"
                     /coded_by="XM_077645334.1:788..2314"
                     /db_xref="GeneID:144112525"
ORIGIN      
        1 mstvrclail nnrrkvinvg gafresdvlq almesdlqqf ipvsariqly dkdfeefvdm
       61 dsesvvqehs klnvivendl gdalnmetpp patgiretar llqkekhnyc lpempyhisr
      121 qlaqisesae vssdlrraii rwlhadllsy gpypgnlyad agrrlvikhp vlsdktgssf
      181 vrlfysfqiy akykcrlfff lipdvrfssa eglgswqial kfrtknerrk itdveevnda
      241 rkkarrsagg cpivaskaat krgeipvndt edehsiqalk efmkketkkc vedqniprlq
      301 damrrtfaer rrwmaqsnat vleviaeypa latleavhee ftritkqnge enllsfinkh
      361 gerllerark lksaaaiika hdeassrams eeekkycfas avlqlmpcfv kenpkrffsq
      421 eenckfpsvv aagdpfqlfp qvaaveglkv dtvdfisala cvfelywafn ihydaqlrqt
      481 favfefafcv easfapgvla trmvtsmk
//
LOCUS       XP_077501461             191 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112526 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501461
VERSION     XP_077501461.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645335.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526863) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..191
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..191
                     /product="uncharacterized protein LOC144112526 isoform X1"
                     /calculated_mol_wt=20931
     CDS             1..191
                     /gene="LOC144112526"
                     /coded_by="XM_077645335.1:292..867"
                     /db_xref="GeneID:144112526"
ORIGIN      
        1 mscsaastlp pssarcskki lmflltylkt skfgtgkksv sedcqvriad vldepqqaeq
       61 srvtatyslt yslpavpndi qfsieptkvd stskhaaels ngsimtsayt pctlasctmr
      121 lrklwcpgtq twlthlapam tlgeklsvsr qnmsgeksvs srmmgqivhr qknqrkmgkw
      181 prlaempcsv f
//
LOCUS       XP_077501462             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112526 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501462
VERSION     XP_077501462.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645336.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526863) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144112526 isoform X2"
                     /calculated_mol_wt=17205
     CDS             1..158
                     /gene="LOC144112526"
                     /coded_by="XM_077645336.1:285..761"
                     /db_xref="GeneID:144112526"
ORIGIN      
        1 mscsaastlp pssarcskki lmflltylkt skfgtgkksv sedcqvriad vldepqqaeq
       61 srvtatyslt yslpavpndi qfsieptkvd stskhaaels ngsimtsayt pctlasctmr
      121 lrklwcpgtq twlthlapam qlhqtgrmrr vllatske
//
LOCUS       XP_077501463             119 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112526 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501463
VERSION     XP_077501463.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645337.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526863) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..119
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..119
                     /product="uncharacterized protein LOC144112526 isoform X3"
                     /calculated_mol_wt=12673
     CDS             1..119
                     /gene="LOC144112526"
                     /coded_by="XM_077645337.1:292..651"
                     /db_xref="GeneID:144112526"
ORIGIN      
        1 mscsaastlp pssarcskki lmflltylkt skfgtgkksv sedcqvriad vldepqqaeq
       61 srvtatyslt yslpavpndi qfsieptkvd stskhaaels ngsimtsayt pcnctrrgg
//
LOCUS       XP_077501464             173 aa            linear   INV 25-NOV-2025
DEFINITION  pre-rRNA-processing protein TSR2 homolog, partial [Amblyomma
            americanum].
ACCESSION   XP_077501464
VERSION     XP_077501464.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645338.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526864) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..173
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         <1..173
                     /product="pre-rRNA-processing protein TSR2 homolog"
     Region          1..78
                     /region_name="WGG"
                     /note="Pre-rRNA-processing protein TSR2; pfam10273"
                     /db_xref="CDD:463040"
     CDS             1..173
                     /gene="LOC144112527"
                     /coded_by="XM_077645338.1:<1..522"
                     /db_xref="GeneID:144112527"
ORIGIN      
        1 hvsvktligd whglqiaien gmsgqqarqk eqwlvgvlee yfaengnlhl devadliaev
       61 idnefdiite gdsldqisal lckhyqmcke fkenevlqsl sqrlpptrtl rvsiageyds
      121 ddeaevqnhi ntevssqrgs lllnepgpsr qqqprrqgpd edgwavvrhg rrt
//
LOCUS       XP_077501465            1485 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112529 [Amblyomma americanum].
ACCESSION   XP_077501465
VERSION     XP_077501465.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645339.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526864) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1485
                     /product="uncharacterized protein LOC144112529"
                     /calculated_mol_wt=165329
     Region          <317..>352
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     Region          402..>441
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            416..418
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133137"
     Site            416
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133137"
     Region          617..676
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          693..789
                     /region_name="rve"
                     /note="Integrase core domain; pfam00665"
                     /db_xref="CDD:459897"
     Region          1084..1255
                     /region_name="RT_LTR"
                     /note="Reverse transcriptases (RTs) from retrotransposons
                     and retroviruses which have long terminal repeats (LTRs)
                     in their DNA copies but not in their RNA template. RT
                     catalyzes DNA replication from an RNA template, and is
                     responsible for the replication of...; cd01647"
                     /db_xref="CDD:238825"
     Site            order(1147..1152,1181..1182,1208,1210..1211,1255)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238825"
     Site            order(1147..1152,1181,1210)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238825"
     Site            1182
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238825"
     Region          1355..1475
                     /region_name="RNase_HI_RT_Ty3"
                     /note="Ty3/Gypsy family of RNase HI in long-term repeat
                     retroelements; cd09274"
                     /db_xref="CDD:260006"
     Site            order(1359..1362,1396..1398,1401,1426,1459,1473)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260006"
     CDS             1..1485
                     /gene="LOC144112529"
                     /coded_by="XM_077645339.1:819..5276"
                     /db_xref="GeneID:144112529"
ORIGIN      
        1 mvrtrgmmas tadseteesg mtpktstdet snlgesrapp ggtaqvetgs elpgvaadie
       61 pdsyalrrle ldfelqrlkl eaeeriamrr aelefggarq sppvsdwsgq rqtgldpttq
      121 cakvlkglrl padsdvpmwf devermfaty nvpdssrvhl iiptltervr yllrglgdea
      181 crdyeavkaa vlselqlspp eylerfegas krkdetwaqf asrvktyfey ylrsrqassk
      241 eevvslivad rmkaslsaeg lehirlrege kwlrpdevar amqtfeqakg knrgvrqasa
      301 qpaskeqagp rsaslapsra ahdgkrqptc yvcraaghla kdcprviqkk ddnrrdaran
      361 avtiadpgtv rdapashsvn vlvaeikvpq reeirksrlq qvqldcagvp tvaildtgse
      421 vtvvredlvp nayrettgni rlvaafgnti eaklvslpig lrssalllep qkvriicalt
      481 nklaegvdcl lskedwells qgsqgeavav faetpksdat igqpgsefgt gsetvseaii
      541 letsaeappa elrdeevtda petlsqrekf raaqvsdesl rkawsdaksg kggmfisegl
      601 lyhwdtiaga rvkqlvlpce rrqevlllah eslwgghlgp rktrarikys fywpgleqev
      661 kqhcrtchgc qirsdrsird rvpitpltrp ehpfqvvnvd iigpidvpsa rghkyalclv
      721 dlctrwpevi clrsltarat cdalltvfsr tgvpevicsd qgtnftsqlt qaflarlgcs
      781 prfstpehpe sngtverwnr vlknmifhvv gkdaknwdkl ipfvlwayre vphdttgvap
      841 frllygrnpt gplailqrtw tgeirvpstl reapakylqq lreqmeiaae vagltatarq
      901 gsyaqaynra trvksfnvgd qvllfdtdrg gklspkwlgp fpvvgrerqn syridtgark
      961 tsvvhanrlr pyyarvshvg vvfdedeefg nveyapcaea giqgqtpiea tkmahlkeeq
     1021 rseiaelfar ypevfsetpg iaevgthriv ledgyrpkra fpyrvpellr kevsrqveel
     1081 lelgliypve sdcahpvvcv gkkdgsvrlc vdyralnagt ktdafpmmha qelimkvgqs
     1141 kfitvvdlrr gywqvpveqe sqhltafvth dgqyawrvmp fglknaaatf qrmmnvllsk
     1201 hqgyatayld dvaifsqsws ehlehlesim sllreaklkv ssqkcqigqa rirylghvvg
     1261 ggthgpdpdk laairdihap ktrkelrsfl glcgyyreyv kgyaevaspl taltkrgvpn
     1321 pipwsdeaqr afeqmkmslc ratmlstpdl skpywlftda seiaagacla qmddggrerp
     1381 iafashrftp tqsrwstier eafaviwalk kfdywvfgae invvsdhnpl syltactphg
     1441 akltrwalal qrynvaithr kgannanada lsrlpnvtwe gadra
//
LOCUS       XP_077501466             364 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112533 [Amblyomma americanum].
ACCESSION   XP_077501466
VERSION     XP_077501466.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645340.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526875) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..364
                     /product="uncharacterized protein LOC144112533"
                     /calculated_mol_wt=41274
     Region          <98..>201
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..364
                     /gene="LOC144112533"
                     /coded_by="XM_077645340.1:1..1095"
                     /db_xref="GeneID:144112533"
ORIGIN      
        1 mnswrapgal sghenstrmr ppnplcffvq vssvtslyak ksndvclllf prpgvllcvf
       61 secfdivnql lalsgdveln pgpntrhqvq dslpdvqskq ledmfailet lntrtakmql
      121 dqkafiksid dlkksqlkle edlsemnkrl tnverktvti dktekevhpf qqlvdqlsse
      181 nsqlrarlse ledrqrrdnl lfygipdaqs eswaqseekl vkvlssclei psseflidra
      241 hrlgglasek crpiiakfss fkvkqrilln csklkdekit lsedfsfatr harkklvefg
      301 ksqpltfklr fnklyiknkc ysynhsddsv heipsafplp tdrsedptnd thsasappte
      361 lvtn
//
LOCUS       XP_077501467             211 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like [Amblyomma americanum].
ACCESSION   XP_077501467
VERSION     XP_077501467.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645341.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526878) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..211
                     /product="tubulin beta chain-like"
                     /calculated_mol_wt=24202
     Region          <1..198
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..211
                     /gene="LOC144112535"
                     /coded_by="XM_077645341.1:129..764"
                     /db_xref="GeneID:144112535"
ORIGIN      
        1 msgvttclrf pgqlnadlrk lavnmvpfpr lqffmtgfap ltsrgcqqyr altvpeltqq
       61 mfdaknmmaa cdprhgrylt vaavfrgrms nrevddqmln iqnkdsnyfv ewipnnvkta
      121 vcdipprglk msatfignst aiqelfkris eqftpmfrrk aflhwytgeg mdefefteae
      181 snlnelvsey qqyqeatped eeefeetqae a
//
LOCUS       XP_077501468             297 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-1 chain-like [Amblyomma americanum].
ACCESSION   XP_077501468
VERSION     XP_077501468.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645342.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526878) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..297
                     /product="tubulin beta-1 chain-like"
                     /calculated_mol_wt=33977
     Region          <1..284
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..297
                     /gene="LOC144112536"
                     /coded_by="XM_077645342.1:16..909"
                     /db_xref="GeneID:144112536"
ORIGIN      
        1 mgtlliskir eeypdrimnt ysvvpspkvs dtvvepynat lsvhqlvent detycidnea
       61 lydicfrtlk lttptygdln hlvsatmsgv ttclrfpgql nadlrklavn mvpfprlhff
      121 mtgfapltsr gcqqyraltl peltqqmfda knmmafcdpr hgryltvaav frgrmsmrev
      181 ddqmlniqnk dssyfvewip nnvktavcdi pprglkmsat fignstaiqe lfkriseqft
      241 amfrrkaflh wytgegmdef efteaesnln elvseyqqyq eatpedeeef eetqaea
//
LOCUS       XP_077501469             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-1 chain-like [Amblyomma americanum].
ACCESSION   XP_077501469
VERSION     XP_077501469.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645343.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526878) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-1 chain-like"
                     /calculated_mol_wt=49483
     Region          1..430
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144112539"
                     /coded_by="XM_077645343.1:87..1418"
                     /db_xref="GeneID:144112539"
ORIGIN      
        1 mreivhiqtg qcgnqigakf welisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gavdavrsgp ygplfrpdnf vfgqsgagnn wakghytega elvdsaldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrgcqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkdssy fvewipnnvk tavcdipprg
      361 lkmsatfign staiqdlfkr iseqftamfr rkaflhwytg egmdefefte aesnlnelvs
      421 eyqqyqeatp edeeefeetq aea
//
LOCUS       XP_077501470             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501470
VERSION     XP_077501470.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645344.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526878) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like isoform X1"
                     /calculated_mol_wt=49511
     Region          1..430
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144112540"
                     /coded_by="XM_077645344.1:87..1418"
                     /db_xref="GeneID:144112540"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gavdavrsgp ygplfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrgcqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkdssy fvewipnnvk tavcdipprg
      361 lkmsatfign staiqelfkr iseqftamfr rkaflhwytg egmdefefte aesnlnelvs
      421 eyqqyqeatp edeeefeetq aea
//
LOCUS       XP_077501471             442 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077501471
VERSION     XP_077501471.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645345.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526878) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..442
                     /product="tubulin beta-4 chain-like isoform X2"
                     /calculated_mol_wt=49454
     Region          1..429
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..442
                     /gene="LOC144112540"
                     /coded_by="XM_077645345.1:87..1415"
                     /db_xref="GeneID:144112540"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasgkyvp
       61 railvdlepg avdavrsgpy gplfrpdnfv fgqsgagnnw akghytegae lvdsvldvvr
      121 keaescdclq gfqlthslgg gtgsgmgtll iskireeypd rimntysvvp spkvsdtvve
      181 pynatlsvhq lventdetyc idnealydic frtlklttpt ygdlnhlvsa tmsgvttclr
      241 fpgqlnadlr klavnmvpfp rlhffmtgfa pltsrgcqqy raltvpeltq qmfdaknmma
      301 acdprhgryl tvaavfrgrm smrevddqml niqnkdssyf vewipnnvkt avcdipprgl
      361 kmsatfigns taiqelfkri seqftamfrr kaflhwytge gmdefeftea esnlnelvse
      421 yqqyqeatpe deeefeetqa ea
//
LOCUS       XP_077501472             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501472
VERSION     XP_077501472.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645346.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526878) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like isoform X1"
                     /calculated_mol_wt=49511
     Region          1..430
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144112541"
                     /coded_by="XM_077645346.1:205..1536"
                     /db_xref="GeneID:144112541"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gavdavrsgp ygplfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrgcqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkdssy fvewipnnvk tavcdipprg
      361 lkmsatfign staiqelfkr iseqftamfr rkaflhwytg egmdefefte aesnlnelvs
      421 eyqqyqeatp edeeefeetq aea
//
LOCUS       XP_077501473             442 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077501473
VERSION     XP_077501473.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645347.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526878) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..442
                     /product="tubulin beta-4 chain-like isoform X2"
                     /calculated_mol_wt=49454
     Region          1..429
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..442
                     /gene="LOC144112541"
                     /coded_by="XM_077645347.1:204..1532"
                     /db_xref="GeneID:144112541"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasgkyvp
       61 railvdlepg avdavrsgpy gplfrpdnfv fgqsgagnnw akghytegae lvdsvldvvr
      121 keaescdclq gfqlthslgg gtgsgmgtll iskireeypd rimntysvvp spkvsdtvve
      181 pynatlsvhq lventdetyc idnealydic frtlklttpt ygdlnhlvsa tmsgvttclr
      241 fpgqlnadlr klavnmvpfp rlhffmtgfa pltsrgcqqy raltvpeltq qmfdaknmma
      301 acdprhgryl tvaavfrgrm smrevddqml niqnkdssyf vewipnnvkt avcdipprgl
      361 kmsatfigns taiqelfkri seqftamfrr kaflhwytge gmdefeftea esnlnelvse
      421 yqqyqeatpe deeefeetqa ea
//
LOCUS       XP_077501474             173 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112544 [Amblyomma americanum].
ACCESSION   XP_077501474
VERSION     XP_077501474.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645348.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526879) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 16% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..173
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..173
                     /product="uncharacterized protein LOC144112544"
                     /calculated_mol_wt=18717
     CDS             1..173
                     /gene="LOC144112544"
                     /coded_by="XM_077645348.1:1..522"
                     /db_xref="GeneID:144112544"
ORIGIN      
        1 mlrvfrlnap lrvpgelrfp ggrvhdvrvl glpalqrhgd vaaslkggvl glsgglglde
       61 llvqcqytag prrplqlage vdgrvtnlta rmtlgvnvar atasldvfkf rnignvqvtr
      121 ltgvtppfnw lgvaiinlil qdqadrlrqk leeeakkaln eslentrili tin
//
LOCUS       XP_077501475             683 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: conserved oligomeric Golgi complex subunit
            4-like [Amblyomma americanum].
ACCESSION   XP_077501475
VERSION     XP_077501475.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645349.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526879) and transcript sequence (GBZX01001257.1) annotated
            using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts  :: corrected 1 indel
            assembly gap :: added 371 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /dev_stage="adult"
     Protein         1..683
                     /product="LOW QUALITY PROTEIN: conserved oligomeric Golgi
                     complex subunit 4-like"
                     /calculated_mol_wt=77534
     Region          93..400
                     /region_name="COG4"
                     /note="COG4 transport protein; pfam08318"
                     /db_xref="CDD:462425"
     CDS             1..683
                     /gene="LOC144112546"
                     /coded_by="XM_077645349.1:1..2052"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 1 base in 1 codon;
                     added 371 bases not found in genome assembly"
                     /db_xref="GeneID:144112546"
ORIGIN      
        1 misftstlae nisskvrqld laksrvtecv qrvedildlk fctdgvqtal qnedyeqaaa
       61 hihrflslde svlkrstadi qegssleeaf sslreaenrl rvivmkkfke avnegdiasv
      121 erffkifpll nehdeglhkf slylssqise tahknlkqaq aasstdkrgn viyadtvtll
      181 fegiartvei hqplvetyyg hgrlhtvvel lqkecdcqvk kiledfkknr qfkkkaqqvq
      241 mllrsskqmd kldprdldvl laevallnsr aelylrfirr rvssdfdvay qdpairsdkv
      301 qqfdrkikes dlcksmqeiv styiimeeyf liesvrkaie vdtieensqc ssmlddiffi
      361 lkkclkrafs sasvdracam lnhscsllet dfadelserl klgfppsgil dlsqaysmiq
      421 ssfqqgriqp aetvekarav fvttlnnvem areytktlas slqedlsksf ssatexetak
      481 lescltdlnn sslkfqslls hgvaelcnaa vkphikswad tfqstdhtlt eddftsyean
      541 dgirpflqtf ivtldgalka fkadlipany dslvnltaae ttfqlekalf kcsfnrlggl
      601 qfdkelryli symtsvttws irdkfsrvsq istllnmemv seildiwgtn agpmtwrltp
      661 tevrqvlslr ndfrqedirr lkl
//
LOCUS       XP_077501476             255 aa            linear   INV 25-NOV-2025
DEFINITION  aurora kinase A-like [Amblyomma americanum].
ACCESSION   XP_077501476
VERSION     XP_077501476.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645350.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526879) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..255
                     /product="aurora kinase A-like"
                     /calculated_mol_wt=28170
     Region          <94..226
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     CDS             1..255
                     /gene="LOC144112547"
                     /coded_by="XM_077645350.1:101..868"
                     /db_xref="GeneID:144112547"
ORIGIN      
        1 mprakkafvr refhgnryah rekakgcprp neipnaeras sstsklsrfs lcfqeedvlq
       61 sssqyalmdm dgicaaitqi cvcrecggsv elveivtkry vyqlcqalkc chslkvihrd
      121 ikpenlllgl ngeikladfg wsvhaptsrr vtlcgtpvyl ppemveqcay dekvdhrclg
      181 iliyeflvgk ppfnadtvek igeeilkgqv qfpdhvsada rdligknpar kevpgrsmgc
      241 llgncsisga ilcla
//
LOCUS       XP_077501478             319 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112548 [Amblyomma americanum].
ACCESSION   XP_077501478
VERSION     XP_077501478.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645352.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526879) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..319
                     /product="uncharacterized protein LOC144112548"
                     /calculated_mol_wt=33633
     CDS             1..319
                     /gene="LOC144112548"
                     /coded_by="XM_077645352.1:1508..2467"
                     /db_xref="GeneID:144112548"
ORIGIN      
        1 mlalrqsrps lsglsgirty fsappktspr ptswpgnspl tqvrvnflsl vmrnltgrct
       61 gesspftsgr prllpatkss gvtvispspa sskshllrws psraercral ssitqrsrqy
      121 estsapsllv tgvgtighrv dvcpcpveer csycgqnvgk aalgmaphec kpacmvcgqa
      181 hltgsqackg kfrrlqqapg tpaarslkgl pptrhsespg ivqggasggk agstatnnaa
      241 lpnarrptrt chifpprsrq rgrhqrrhmn rvlalafqag efpdlggsgp staaaskkss
      301 kknpartevp grimgcllg
//
LOCUS       XP_077501479             719 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112551 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501479
VERSION     XP_077501479.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645353.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526880) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..719
                     /product="uncharacterized protein LOC144112551 isoform X1"
                     /calculated_mol_wt=76939
     Region          2..224
                     /region_name="DHPR_SDR_c_like"
                     /note="dihydropteridine reductase (DHPR), classical (c)
                     SDRs; cd05334"
                     /db_xref="CDD:187595"
     Site            order(8..9,11..14,33,46,55,78..81,102,106,127..128,142,
                     146,174..177,179,182)
                     /site_type="other"
                     /note="NAD binding site [chemical binding]"
                     /db_xref="CDD:187595"
     Site            order(56,86..89,92..94,96..97,108,112,115,132,134..137,
                     140,143..144,148,151..152,154..156,158..162)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187595"
     Site            order(142,146)
                     /site_type="active"
                     /db_xref="CDD:187595"
     Region          <430..576
                     /region_name="LGT"
                     /note="Prolipoprotein diacylglyceryl transferase; cl00478"
                     /db_xref="CDD:469786"
     Region          <514..671
                     /region_name="rne"
                     /note="ribonuclease E; Reviewed; PRK10811"
                     /db_xref="CDD:236766"
     CDS             1..719
                     /gene="LOC144112551"
                     /coded_by="XM_077645353.1:147..2306"
                     /db_xref="GeneID:144112551"
ORIGIN      
        1 magrvvvygg kgalgsaivs ffrqkqwwva svdhlpneea danvvvqpcd awasqhervl
       61 egvdkvlqdg kidalicvag gwaggnaasv dyvkncdslw kssvwtsvia sslaskylke
      121 ggllaltgak aaleptpgmi gygmakaavh hlvqslagak sglpqgatvv ailpvtldtp
      181 mnrkfmpkad fsswtplsfv aelffkwtag qerpangslv klvteggrts vqilklaatk
      241 eemeremrvk rvhvppirms evdnvtecgv paaarkkslk qqekakqqnq itskkqqyed
      301 vlkrhvshsl qknaaipghs sigrkaskkr kpqilddste sedesvvssr elknavkeak
      361 ywkqryrleq ehtaslkrel dflkrkvelq lssfqqtlde vakrqqdlca dalpvvapsi
      421 lpngaveasr rdptaargaa teqaaaaekd vggaasvvpt pagpseeapa sscftadapr
      481 saeleasvld daqdftltsg glapmdvaes qetlagpsqq epmdvtesqe tlagpsqqep
      541 mdvtesqetl agpsqqepmd vtesqetlag psqqepmdvt esqetlagps qqepmdvtes
      601 qetlagpsqq epmdvtesqe tlagpsqqep mdvtesqetl agpsqqapmd vtesqetlpg
      661 psqqasmdva tmldiqpgps qafqltselp tlptapvsvg tprirrpamv ngpvkcayf
//
LOCUS       XP_077501480             715 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112551 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501480
VERSION     XP_077501480.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645354.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526880) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..715
                     /product="uncharacterized protein LOC144112551 isoform X2"
                     /calculated_mol_wt=76536
     Region          2..224
                     /region_name="DHPR_SDR_c_like"
                     /note="dihydropteridine reductase (DHPR), classical (c)
                     SDRs; cd05334"
                     /db_xref="CDD:187595"
     Site            order(8..9,11..14,33,46,55,78..81,102,106,127..128,142,
                     146,174..177,179,182)
                     /site_type="other"
                     /note="NAD binding site [chemical binding]"
                     /db_xref="CDD:187595"
     Site            order(56,86..89,92..94,96..97,108,112,115,132,134..137,
                     140,143..144,148,151..152,154..156,158..162)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187595"
     Site            order(142,146)
                     /site_type="active"
                     /db_xref="CDD:187595"
     Region          <426..572
                     /region_name="LGT"
                     /note="Prolipoprotein diacylglyceryl transferase; cl00478"
                     /db_xref="CDD:469786"
     Region          <510..667
                     /region_name="rne"
                     /note="ribonuclease E; Reviewed; PRK10811"
                     /db_xref="CDD:236766"
     CDS             1..715
                     /gene="LOC144112551"
                     /coded_by="XM_077645354.1:147..2294"
                     /db_xref="GeneID:144112551"
ORIGIN      
        1 magrvvvygg kgalgsaivs ffrqkqwwva svdhlpneea danvvvqpcd awasqhervl
       61 egvdkvlqdg kidalicvag gwaggnaasv dyvkncdslw kssvwtsvia sslaskylke
      121 ggllaltgak aaleptpgmi gygmakaavh hlvqslagak sglpqgatvv ailpvtldtp
      181 mnrkfmpkad fsswtplsfv aelffkwtag qerpangslv klvteggrts vqilklaatk
      241 eemeremrvk rvhvppirms evdnvtecgv paaarkkslk qqekakqqnq itskkqqyed
      301 vlkrhvshsl qknaaipghs sigrkaskkr kpqilddste sedesvvssr elknavkeak
      361 ywkqryrleq ehtaslkrel dflkrkvelq lssfqqtlde vakrqqdalp vvapsilpng
      421 aveasrrdpt aargaateqa aaaekdvgga asvvptpagp seeapasscf tadaprsael
      481 easvlddaqd ftltsgglap mdvaesqetl agpsqqepmd vtesqetlag psqqepmdvt
      541 esqetlagps qqepmdvtes qetlagpsqq epmdvtesqe tlagpsqqep mdvtesqetl
      601 agpsqqepmd vtesqetlag psqqepmdvt esqetlagps qqapmdvtes qetlpgpsqq
      661 asmdvatmld iqpgpsqafq ltselptlpt apvsvgtpri rrpamvngpv kcayf
//
LOCUS       XP_077501481             706 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112551 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501481
VERSION     XP_077501481.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645355.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526880) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..706
                     /product="uncharacterized protein LOC144112551 isoform X3"
                     /calculated_mol_wt=75414
     Region          2..224
                     /region_name="DHPR_SDR_c_like"
                     /note="dihydropteridine reductase (DHPR), classical (c)
                     SDRs; cd05334"
                     /db_xref="CDD:187595"
     Site            order(8..9,11..14,33,46,55,78..81,102,106,127..128,142,
                     146,174..177,179,182)
                     /site_type="other"
                     /note="NAD binding site [chemical binding]"
                     /db_xref="CDD:187595"
     Site            order(56,86..89,92..94,96..97,108,112,115,132,134..137,
                     140,143..144,148,151..152,154..156,158..162)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187595"
     Site            order(142,146)
                     /site_type="active"
                     /db_xref="CDD:187595"
     Region          <417..563
                     /region_name="LGT"
                     /note="Prolipoprotein diacylglyceryl transferase; cl00478"
                     /db_xref="CDD:469786"
     Region          <501..658
                     /region_name="rne"
                     /note="ribonuclease E; Reviewed; PRK10811"
                     /db_xref="CDD:236766"
     CDS             1..706
                     /gene="LOC144112551"
                     /coded_by="XM_077645355.1:147..2267"
                     /db_xref="GeneID:144112551"
ORIGIN      
        1 magrvvvygg kgalgsaivs ffrqkqwwva svdhlpneea danvvvqpcd awasqhervl
       61 egvdkvlqdg kidalicvag gwaggnaasv dyvkncdslw kssvwtsvia sslaskylke
      121 ggllaltgak aaleptpgmi gygmakaavh hlvqslagak sglpqgatvv ailpvtldtp
      181 mnrkfmpkad fsswtplsfv aelffkwtag qerpangslv klvteggrts vqilklaatk
      241 eemeremrvk rvhvppirms evdnvtecgv paaarkkslk qqekakqqnq itskkqqyed
      301 vlkrhvshsl qknaaipghs sigrkaskkr kpqilddste sedesvvssr elknavkeak
      361 ywkqryrleq ehtaslkrel dflkrkvelq lssylcadal pvvapsilpn gaveasrrdp
      421 taargaateq aaaaekdvgg aasvvptpag pseeapassc ftadaprsae leasvlddaq
      481 dftltsggla pmdvaesqet lagpsqqepm dvtesqetla gpsqqepmdv tesqetlagp
      541 sqqepmdvte sqetlagpsq qepmdvtesq etlagpsqqe pmdvtesqet lagpsqqepm
      601 dvtesqetla gpsqqepmdv tesqetlagp sqqapmdvte sqetlpgpsq qasmdvatml
      661 diqpgpsqaf qltselptlp tapvsvgtpr irrpamvngp vkcayf
//
LOCUS       XP_077501482             702 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112551 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077501482
VERSION     XP_077501482.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645356.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526880) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..702
                     /product="uncharacterized protein LOC144112551 isoform X4"
                     /calculated_mol_wt=75012
     Region          2..224
                     /region_name="DHPR_SDR_c_like"
                     /note="dihydropteridine reductase (DHPR), classical (c)
                     SDRs; cd05334"
                     /db_xref="CDD:187595"
     Site            order(8..9,11..14,33,46,55,78..81,102,106,127..128,142,
                     146,174..177,179,182)
                     /site_type="other"
                     /note="NAD binding site [chemical binding]"
                     /db_xref="CDD:187595"
     Site            order(56,86..89,92..94,96..97,108,112,115,132,134..137,
                     140,143..144,148,151..152,154..156,158..162)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187595"
     Site            order(142,146)
                     /site_type="active"
                     /db_xref="CDD:187595"
     Region          336..>698
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          <497..654
                     /region_name="rne"
                     /note="ribonuclease E; Reviewed; PRK10811"
                     /db_xref="CDD:236766"
     CDS             1..702
                     /gene="LOC144112551"
                     /coded_by="XM_077645356.1:147..2255"
                     /db_xref="GeneID:144112551"
ORIGIN      
        1 magrvvvygg kgalgsaivs ffrqkqwwva svdhlpneea danvvvqpcd awasqhervl
       61 egvdkvlqdg kidalicvag gwaggnaasv dyvkncdslw kssvwtsvia sslaskylke
      121 ggllaltgak aaleptpgmi gygmakaavh hlvqslagak sglpqgatvv ailpvtldtp
      181 mnrkfmpkad fsswtplsfv aelffkwtag qerpangslv klvteggrts vqilklaatk
      241 eemeremrvk rvhvppirms evdnvtecgv paaarkkslk qqekakqqnq itskkqqyed
      301 vlkrhvshsl qknaaipghs sigrkaskkr kpqilddste sedesvvssr elknavkeak
      361 ywkqryrleq ehtaslkrel dflkrkvelq lssyalpvva psilpngave asrrdptaar
      421 gaateqaaaa ekdvggaasv vptpagpsee apasscftad aprsaeleas vlddaqdftl
      481 tsgglapmdv aesqetlagp sqqepmdvte sqetlagpsq qepmdvtesq etlagpsqqe
      541 pmdvtesqet lagpsqqepm dvtesqetla gpsqqepmdv tesqetlagp sqqepmdvte
      601 sqetlagpsq qepmdvtesq etlagpsqqa pmdvtesqet lpgpsqqasm dvatmldiqp
      661 gpsqafqlts elptlptapv svgtprirrp amvngpvkca yf
//
LOCUS       XP_077501483             675 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112551 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077501483
VERSION     XP_077501483.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645357.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526880) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..675
                     /product="uncharacterized protein LOC144112551 isoform X5"
                     /calculated_mol_wt=71931
     Region          2..224
                     /region_name="DHPR_SDR_c_like"
                     /note="dihydropteridine reductase (DHPR), classical (c)
                     SDRs; cd05334"
                     /db_xref="CDD:187595"
     Site            order(8..9,11..14,33,46,55,78..81,102,106,127..128,142,
                     146,174..177,179,182)
                     /site_type="other"
                     /note="NAD binding site [chemical binding]"
                     /db_xref="CDD:187595"
     Site            order(56,86..89,92..94,96..97,108,112,115,132,134..137,
                     140,143..144,148,151..152,154..156,158..162)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187595"
     Site            order(142,146)
                     /site_type="active"
                     /db_xref="CDD:187595"
     Region          <386..532
                     /region_name="LGT"
                     /note="Prolipoprotein diacylglyceryl transferase; cl00478"
                     /db_xref="CDD:469786"
     Region          <470..627
                     /region_name="rne"
                     /note="ribonuclease E; Reviewed; PRK10811"
                     /db_xref="CDD:236766"
     CDS             1..675
                     /gene="LOC144112551"
                     /coded_by="XM_077645357.1:147..2174"
                     /db_xref="GeneID:144112551"
ORIGIN      
        1 magrvvvygg kgalgsaivs ffrqkqwwva svdhlpneea danvvvqpcd awasqhervl
       61 egvdkvlqdg kidalicvag gwaggnaasv dyvkncdslw kssvwtsvia sslaskylke
      121 ggllaltgak aaleptpgmi gygmakaavh hlvqslagak sglpqgatvv ailpvtldtp
      181 mnrkfmpkad fsswtplsfv aelffkwtag qerpangslv klvteggrts vqilklaatk
      241 eemeremrvk rvhvppirms evdnvtecgv paaarkkslk qaskkrkpqi lddstesede
      301 svvssrelkn avkeakywkq ryrleqehta slkreldflk rkvelqlssf qqtldevakr
      361 qqdlcadalp vvapsilpng aveasrrdpt aargaateqa aaaekdvgga asvvptpagp
      421 seeapasscf tadaprsael easvlddaqd ftltsgglap mdvaesqetl agpsqqepmd
      481 vtesqetlag psqqepmdvt esqetlagps qqepmdvtes qetlagpsqq epmdvtesqe
      541 tlagpsqqep mdvtesqetl agpsqqepmd vtesqetlag psqqepmdvt esqetlagps
      601 qqapmdvtes qetlpgpsqq asmdvatmld iqpgpsqafq ltselptlpt apvsvgtpri
      661 rrpamvngpv kcayf
//
LOCUS       XP_077501484             629 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112551 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077501484
VERSION     XP_077501484.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645358.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526880) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..629
                     /product="uncharacterized protein LOC144112551 isoform X6"
                     /calculated_mol_wt=67293
     Region          2..224
                     /region_name="DHPR_SDR_c_like"
                     /note="dihydropteridine reductase (DHPR), classical (c)
                     SDRs; cd05334"
                     /db_xref="CDD:187595"
     Site            order(8..9,11..14,33,46,55,78..81,102,106,127..128,142,
                     146,174..177,179,182)
                     /site_type="other"
                     /note="NAD binding site [chemical binding]"
                     /db_xref="CDD:187595"
     Site            order(56,86..89,92..94,96..97,108,112,115,132,134..137,
                     140,143..144,148,151..152,154..156,158..162)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187595"
     Site            order(142,146)
                     /site_type="active"
                     /db_xref="CDD:187595"
     Region          <414..>627
                     /region_name="PRK07003"
                     /note="DNA polymerase III subunit gamma/tau"
                     /db_xref="CDD:235906"
     CDS             1..629
                     /gene="LOC144112551"
                     /coded_by="XM_077645358.1:146..2035"
                     /db_xref="GeneID:144112551"
ORIGIN      
        1 magrvvvygg kgalgsaivs ffrqkqwwva svdhlpneea danvvvqpcd awasqhervl
       61 egvdkvlqdg kidalicvag gwaggnaasv dyvkncdslw kssvwtsvia sslaskylke
      121 ggllaltgak aaleptpgmi gygmakaavh hlvqslagak sglpqgatvv ailpvtldtp
      181 mnrkfmpkad fsswtplsfv aelffkwtag qerpangslv klvteggrts vqilklaatk
      241 eemeremrvk rvhvppirms evdnvtecgv paaarkkslk qqekakqqnq itskkqqyed
      301 vlkrhvshsl qknaaipghs sigrkaskkr kpqilddste sedesvvssr elknavkeak
      361 ywkqryrleq ehtaslkrel dflkrkvelq lssfqqtlde vakrqqdlca dalpvvapsi
      421 lpngaveasr rdptaargaa teqaaaaekd vggaasvvpt pagpseeapa sscftadapr
      481 saeleasvld daqdftltsg glapmdvaes qetlagpsqq epmdvtesqe tlagpsqqep
      541 mdvtesqetl agpsqqapmd vtesqetlpg psqqasmdva tmldiqpgps qafqltselp
      601 tlptapvsvg tprirrpamv ngpvkcayf
//
LOCUS       XP_077501485             623 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112551 isoform X7 [Amblyomma
            americanum].
ACCESSION   XP_077501485
VERSION     XP_077501485.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645359.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526880) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..623
                     /product="uncharacterized protein LOC144112551 isoform X7"
                     /calculated_mol_wt=68578
     Region          2..224
                     /region_name="DHPR_SDR_c_like"
                     /note="dihydropteridine reductase (DHPR), classical (c)
                     SDRs; cd05334"
                     /db_xref="CDD:187595"
     Site            order(8..9,11..14,33,46,55,78..81,102,106,127..128,142,
                     146,174..177,179,182)
                     /site_type="other"
                     /note="NAD binding site [chemical binding]"
                     /db_xref="CDD:187595"
     Site            order(56,86..89,92..94,96..97,108,112,115,132,134..137,
                     140,143..144,148,151..152,154..156,158..162)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187595"
     Site            order(142,146)
                     /site_type="active"
                     /db_xref="CDD:187595"
     CDS             1..623
                     /gene="LOC144112551"
                     /coded_by="XM_077645359.1:146..2017"
                     /db_xref="GeneID:144112551"
ORIGIN      
        1 magrvvvygg kgalgsaivs ffrqkqwwva svdhlpneea danvvvqpcd awasqhervl
       61 egvdkvlqdg kidalicvag gwaggnaasv dyvkncdslw kssvwtsvia sslaskylke
      121 ggllaltgak aaleptpgmi gygmakaavh hlvqslagak sglpqgatvv ailpvtldtp
      181 mnrkfmpkad fsswtplsfv aelffkwtag qerpangslv klvteggrts vqilklaatk
      241 eemeremrvk rvhvppirms evdnvtecgv paaarkkslk qqekakqqnq itskkqqyed
      301 vlkrhvshsl qknaaipghs sigrkaskkr kpqilddste sedesvvssr elknavkeak
      361 ywkqryrleq ehtaslkrel dflkrkvelq lssfqqtlde vakrqqdlca dalpvvapsi
      421 lpngaveasr rdptaargaa teqaaaaekd vggaasvvpt pagpseeapa sscftadapr
      481 saeleasvld daqdftltsg glapmdvaes qetlagpsqq pgdwqahhnr nlwmslkarr
      541 lwqahhnrnl wmslkarrlw qahhnrhlwm slkarklyqa hhnrhlwmwp qcwtfsqghl
      601 kpfnlhqnfr lcqppqsllg rhe
//
LOCUS       XP_077501486             623 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112551 isoform X8 [Amblyomma
            americanum].
ACCESSION   XP_077501486
VERSION     XP_077501486.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645360.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526880) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..623
                     /product="uncharacterized protein LOC144112551 isoform X8"
                     /calculated_mol_wt=68578
     Region          2..224
                     /region_name="DHPR_SDR_c_like"
                     /note="dihydropteridine reductase (DHPR), classical (c)
                     SDRs; cd05334"
                     /db_xref="CDD:187595"
     Site            order(8..9,11..14,33,46,55,78..81,102,106,127..128,142,
                     146,174..177,179,182)
                     /site_type="other"
                     /note="NAD binding site [chemical binding]"
                     /db_xref="CDD:187595"
     Site            order(56,86..89,92..94,96..97,108,112,115,132,134..137,
                     140,143..144,148,151..152,154..156,158..162)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187595"
     Site            order(142,146)
                     /site_type="active"
                     /db_xref="CDD:187595"
     CDS             1..623
                     /gene="LOC144112551"
                     /coded_by="XM_077645360.1:146..2017"
                     /db_xref="GeneID:144112551"
ORIGIN      
        1 magrvvvygg kgalgsaivs ffrqkqwwva svdhlpneea danvvvqpcd awasqhervl
       61 egvdkvlqdg kidalicvag gwaggnaasv dyvkncdslw kssvwtsvia sslaskylke
      121 ggllaltgak aaleptpgmi gygmakaavh hlvqslagak sglpqgatvv ailpvtldtp
      181 mnrkfmpkad fsswtplsfv aelffkwtag qerpangslv klvteggrts vqilklaatk
      241 eemeremrvk rvhvppirms evdnvtecgv paaarkkslk qqekakqqnq itskkqqyed
      301 vlkrhvshsl qknaaipghs sigrkaskkr kpqilddste sedesvvssr elknavkeak
      361 ywkqryrleq ehtaslkrel dflkrkvelq lssfqqtlde vakrqqdlca dalpvvapsi
      421 lpngaveasr rdptaargaa teqaaaaekd vggaasvvpt pagpseeapa sscftadapr
      481 saeleasvld daqdftltsg glapmdvaes qetlagpsqq pgdwqahhnr nlwmslkarr
      541 lwqahhnrnl wmslkarrlw qahhnrhlwm slkarklyqa hhnrhlwmwp qcwtfsqghl
      601 kpfnlhqnfr lcqppqsllg rhe
//
LOCUS       XP_077501487             623 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112551 isoform X9 [Amblyomma
            americanum].
ACCESSION   XP_077501487
VERSION     XP_077501487.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645361.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526880) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..623
                     /product="uncharacterized protein LOC144112551 isoform X9"
                     /calculated_mol_wt=68578
     Region          2..224
                     /region_name="DHPR_SDR_c_like"
                     /note="dihydropteridine reductase (DHPR), classical (c)
                     SDRs; cd05334"
                     /db_xref="CDD:187595"
     Site            order(8..9,11..14,33,46,55,78..81,102,106,127..128,142,
                     146,174..177,179,182)
                     /site_type="other"
                     /note="NAD binding site [chemical binding]"
                     /db_xref="CDD:187595"
     Site            order(56,86..89,92..94,96..97,108,112,115,132,134..137,
                     140,143..144,148,151..152,154..156,158..162)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187595"
     Site            order(142,146)
                     /site_type="active"
                     /db_xref="CDD:187595"
     CDS             1..623
                     /gene="LOC144112551"
                     /coded_by="XM_077645361.1:146..2017"
                     /db_xref="GeneID:144112551"
ORIGIN      
        1 magrvvvygg kgalgsaivs ffrqkqwwva svdhlpneea danvvvqpcd awasqhervl
       61 egvdkvlqdg kidalicvag gwaggnaasv dyvkncdslw kssvwtsvia sslaskylke
      121 ggllaltgak aaleptpgmi gygmakaavh hlvqslagak sglpqgatvv ailpvtldtp
      181 mnrkfmpkad fsswtplsfv aelffkwtag qerpangslv klvteggrts vqilklaatk
      241 eemeremrvk rvhvppirms evdnvtecgv paaarkkslk qqekakqqnq itskkqqyed
      301 vlkrhvshsl qknaaipghs sigrkaskkr kpqilddste sedesvvssr elknavkeak
      361 ywkqryrleq ehtaslkrel dflkrkvelq lssfqqtlde vakrqqdlca dalpvvapsi
      421 lpngaveasr rdptaargaa teqaaaaekd vggaasvvpt pagpseeapa sscftadapr
      481 saeleasvld daqdftltsg glapmdvaes qetlagpsqq pgdwqahhnr nlwmslkarr
      541 lwqahhnrnl wmslkarrlw qahhnrhlwm slkarklyqa hhnrhlwmwp qcwtfsqghl
      601 kpfnlhqnfr lcqppqsllg rhe
//
LOCUS       XP_077501489             611 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112551 isoform X10 [Amblyomma
            americanum].
ACCESSION   XP_077501489
VERSION     XP_077501489.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645363.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526880) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..611
                     /product="uncharacterized protein LOC144112551 isoform
                     X10"
                     /calculated_mol_wt=65364
     Region          2..224
                     /region_name="DHPR_SDR_c_like"
                     /note="dihydropteridine reductase (DHPR), classical (c)
                     SDRs; cd05334"
                     /db_xref="CDD:187595"
     Site            order(8..9,11..14,33,46,55,78..81,102,106,127..128,142,
                     146,174..177,179,182)
                     /site_type="other"
                     /note="NAD binding site [chemical binding]"
                     /db_xref="CDD:187595"
     Site            order(56,86..89,92..94,96..97,108,112,115,132,134..137,
                     140,143..144,148,151..152,154..156,158..162)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187595"
     Site            order(142,146)
                     /site_type="active"
                     /db_xref="CDD:187595"
     CDS             1..611
                     /gene="LOC144112551"
                     /coded_by="XM_077645363.1:145..1980"
                     /db_xref="GeneID:144112551"
ORIGIN      
        1 magrvvvygg kgalgsaivs ffrqkqwwva svdhlpneea danvvvqpcd awasqhervl
       61 egvdkvlqdg kidalicvag gwaggnaasv dyvkncdslw kssvwtsvia sslaskylke
      121 ggllaltgak aaleptpgmi gygmakaavh hlvqslagak sglpqgatvv ailpvtldtp
      181 mnrkfmpkad fsswtplsfv aelffkwtag qerpangslv klvteggrts vqilklaatk
      241 eemeremrvk rvhvppirms evdnvtecgv paaarkkslk qqekakqqnq itskkqqyed
      301 vlkrhvshsl qknaaipghs sigrkaskkr kpqilddste sedesvvssr elknavkeak
      361 ywkqryrleq ehtaslkrel dflkrkvelq lssfqqtlde vakrqqdlca dalpvvapsi
      421 lpngaveasr rdptaargaa teqaaaaekd vggaasvvpt pagpseeapa sscftadapr
      481 saeleasvld daqdftltsg glapmdvaes qetlagpsqq epmdvtesqe tlagpsqqap
      541 mdvtesqetl pgpsqqasmd vatmldiqpg psqafqltse lptlptapvs vgtprirrpa
      601 mvngpvkcay f
//
LOCUS       XP_077501490             611 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112551 isoform X11 [Amblyomma
            americanum].
ACCESSION   XP_077501490
VERSION     XP_077501490.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645364.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526880) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..611
                     /product="uncharacterized protein LOC144112551 isoform
                     X11"
                     /calculated_mol_wt=65364
     Region          2..224
                     /region_name="DHPR_SDR_c_like"
                     /note="dihydropteridine reductase (DHPR), classical (c)
                     SDRs; cd05334"
                     /db_xref="CDD:187595"
     Site            order(8..9,11..14,33,46,55,78..81,102,106,127..128,142,
                     146,174..177,179,182)
                     /site_type="other"
                     /note="NAD binding site [chemical binding]"
                     /db_xref="CDD:187595"
     Site            order(56,86..89,92..94,96..97,108,112,115,132,134..137,
                     140,143..144,148,151..152,154..156,158..162)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187595"
     Site            order(142,146)
                     /site_type="active"
                     /db_xref="CDD:187595"
     CDS             1..611
                     /gene="LOC144112551"
                     /coded_by="XM_077645364.1:145..1980"
                     /db_xref="GeneID:144112551"
ORIGIN      
        1 magrvvvygg kgalgsaivs ffrqkqwwva svdhlpneea danvvvqpcd awasqhervl
       61 egvdkvlqdg kidalicvag gwaggnaasv dyvkncdslw kssvwtsvia sslaskylke
      121 ggllaltgak aaleptpgmi gygmakaavh hlvqslagak sglpqgatvv ailpvtldtp
      181 mnrkfmpkad fsswtplsfv aelffkwtag qerpangslv klvteggrts vqilklaatk
      241 eemeremrvk rvhvppirms evdnvtecgv paaarkkslk qqekakqqnq itskkqqyed
      301 vlkrhvshsl qknaaipghs sigrkaskkr kpqilddste sedesvvssr elknavkeak
      361 ywkqryrleq ehtaslkrel dflkrkvelq lssfqqtlde vakrqqdlca dalpvvapsi
      421 lpngaveasr rdptaargaa teqaaaaekd vggaasvvpt pagpseeapa sscftadapr
      481 saeleasvld daqdftltsg glapmdvaes qetlagpsqq epmdvtesqe tlagpsqqap
      541 mdvtesqetl pgpsqqasmd vatmldiqpg psqafqltse lptlptapvs vgtprirrpa
      601 mvngpvkcay f
//
LOCUS       XP_077501491             711 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112553 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501491
VERSION     XP_077501491.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645365.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526880) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..711
                     /product="uncharacterized protein LOC144112553 isoform X1"
                     /calculated_mol_wt=76404
     Region          60..256
                     /region_name="DHPR_SDR_c_like"
                     /note="dihydropteridine reductase (DHPR), classical (c)
                     SDRs; cd05334"
                     /db_xref="CDD:187595"
     Site            order(88,118..121,124..126,128..129,140,144,147,164,
                     166..169,172,175..176,180,183..184,186..188,190..194)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187595"
     Site            order(174,178)
                     /site_type="active"
                     /db_xref="CDD:187595"
     Region          <458..604
                     /region_name="LGT"
                     /note="Prolipoprotein diacylglyceryl transferase; cl00478"
                     /db_xref="CDD:469786"
     CDS             1..711
                     /gene="LOC144112553"
                     /coded_by="XM_077645365.1:25..2160"
                     /db_xref="GeneID:144112553"
ORIGIN      
        1 mvrqpagcps vvnkskrpin aahvtncker wgrrnrlgcl gffrrpvsas rkptpgeytw
       61 vasvdhlpne eadanvvvqp cdawasqher vlegvdkvlq dgkidalicv aggwaggnaa
      121 svdyvkncds lwkssvwtsv iasslaskyl keggllaltg akaaleptpg migygmakaa
      181 vhhlvqslag aksglpqgat vvailpvtld tpmnrkfmpk adfsswtpls fvaelffkwt
      241 agqerpangs lvklvteggr tsvqilklaa tkeemeremr vkrvhvppir msevdnvtec
      301 gvpaaarkks lkqqekakqq nqitskkqqy edvlkrhvsh slqknaaipg hssigrkask
      361 krkpqildds tesedesvvs srelknavke akywkqryrl eqehtaslkr eldflkrkve
      421 lqlssfqqtl devakrqqda lpvvapsilp ngaveasrrd ptaargaate qaaaaekdvg
      481 gaasvvptpa gpseeapass cftadaprsa eleasvldda qdftltsggl apmdvaesqe
      541 tlagpsqqep mdvtesqetl agpsqqepmd vtesqetlag psqqepmdvt esqetlagps
      601 qqepmdvtes qetlagpsqq epmdvtesqe tlagpsqqap mdvtesqetl pgpsqqasmd
      661 vatmldiqpg psqafqltse lptlptapvs vgtprirrpa mvngpvkcay f
//
LOCUS       XP_077501492             679 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112553 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501492
VERSION     XP_077501492.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645366.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526880) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..679
                     /product="uncharacterized protein LOC144112553 isoform X2"
                     /calculated_mol_wt=72678
     Region          2..224
                     /region_name="DHPR_SDR_c_like"
                     /note="dihydropteridine reductase (DHPR), classical (c)
                     SDRs; cd05334"
                     /db_xref="CDD:187595"
     Site            order(8..9,11..14,33,46,55,78..81,102,106,127..128,142,
                     146,174..177,179,182)
                     /site_type="other"
                     /note="NAD binding site [chemical binding]"
                     /db_xref="CDD:187595"
     Site            order(56,86..89,92..94,96..97,108,112,115,132,134..137,
                     140,143..144,148,151..152,154..156,158..162)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187595"
     Site            order(142,146)
                     /site_type="active"
                     /db_xref="CDD:187595"
     Region          <426..572
                     /region_name="LGT"
                     /note="Prolipoprotein diacylglyceryl transferase; cl00478"
                     /db_xref="CDD:469786"
     CDS             1..679
                     /gene="LOC144112553"
                     /coded_by="XM_077645366.1:148..2187"
                     /db_xref="GeneID:144112553"
ORIGIN      
        1 magrvvvygg kgalgsaivs ffrqkqwwva svdhlpneea danvvvqpcd awasqhervl
       61 egvdkvlqdg kidalicvag gwaggnaasv dyvkncdslw kssvwtsvia sslaskylke
      121 ggllaltgak aaleptpgmi gygmakaavh hlvqslagak sglpqgatvv ailpvtldtp
      181 mnrkfmpkad fsswtplsfv aelffkwtag qerpangslv klvteggrts vqilklaatk
      241 eemeremrvk rvhvppirms evdnvtecgv paaarkkslk qqekakqqnq itskkqqyed
      301 vlkrhvshsl qknaaipghs sigrkaskkr kpqilddste sedesvvssr elknavkeak
      361 ywkqryrleq ehtaslkrel dflkrkvelq lssfqqtlde vakrqqdalp vvapsilpng
      421 aveasrrdpt aargaateqa aaaekdvgga asvvptpagp seeapasscf tadaprsael
      481 easvlddaqd ftltsgglap mdvaesqetl agpsqqepmd vtesqetlag psqqepmdvt
      541 esqetlagps qqepmdvtes qetlagpsqq epmdvtesqe tlagpsqqep mdvtesqetl
      601 agpsqqapmd vtesqetlpg psqqasmdva tmldiqpgps qafqltselp tlptapvsvg
      661 tprirrpamv ngpvkcayf
//
LOCUS       XP_077501493             142 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112554 [Amblyomma americanum].
ACCESSION   XP_077501493
VERSION     XP_077501493.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645367.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526880) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..142
                     /product="uncharacterized protein LOC144112554"
                     /calculated_mol_wt=15410
     CDS             1..142
                     /gene="LOC144112554"
                     /coded_by="XM_077645367.1:193..621"
                     /db_xref="GeneID:144112554"
ORIGIN      
        1 mkrlsgvhvl nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar
       61 pgevyvvhrc rrcgakghkt dhcgptahra atplqvavev llktalfrat slfpgrsras
      121 rfvpvfplcr nrrqtsksrg vs
//
LOCUS       XP_077501494             173 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112556, partial [Amblyomma
            americanum].
ACCESSION   XP_077501494
VERSION     XP_077501494.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645368.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526881) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: incomplete on the carboxy end.
FEATURES             Location/Qualifiers
     source          1..173
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..>173
                     /product="uncharacterized protein LOC144112556"
     Region          <1..61
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..173
                     /gene="LOC144112556"
                     /coded_by="XM_077645368.1:1..>520"
                     /db_xref="GeneID:144112556"
ORIGIN      
        1 mfrfpadsnr rkrwlaqvkr dcweptaasr icsahfeets feqnrqdglk klrpdavptl
       61 ftyraipkhr kplkkrscfr patptqspvl dptgnepcgs aalepvfast aeqtqdsavs
      121 dsmssqdtvs plqcaqvpgy dlpltttllp pkavdtviya dsvahsflrt dka
//
LOCUS       XP_077501496             553 aa            linear   INV 25-NOV-2025
DEFINITION  neprilysin-1-like [Amblyomma americanum].
ACCESSION   XP_077501496
VERSION     XP_077501496.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645370.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526885) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..553
                     /product="neprilysin-1-like"
                     /calculated_mol_wt=61408
     Region          93..>549
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..553
                     /gene="LOC144112561"
                     /coded_by="XM_077645370.1:1..1662"
                     /db_xref="GeneID:144112561"
ORIGIN      
        1 magegckaps ttsggldrrq ravalghlls sllgvaltls aitiglsvlr hqrgsegsgv
       61 fgmgrsswnt cnsklcmahf rrlkdslnrs tapcgdfyrf acgawhpraa ksetvfrdll
      121 avaqqdairy leavndasnk mvlptgkvll pdsppvlrin raerahqvcl hsgsstkdaa
      181 lltefladrs lswperttap aqpldvlldl dinwnlglwf svrvspipgc nqrqvrvgsg
      241 ivsydwkral dtmvtrgvyk arvcrfhdal rarsfaamsd kemkelrrye stivgaqcgs
      301 vsggqnelaf plkqmqnlat prlaaalwdt llnwqlnkal rvteadrvia tsvkllqavd
      361 yllsslpaev vlhhlawslv qmigwmadsa lpgrptayem edmrsadcfw vthrafgvth
      421 lsgyinttyp lhwrssvdai lmtitqtain fflktswiha esraaavdkl rrmntslwps
      481 dsylnaseva ailenfpepr edgavlrywl dalsarrall grqwdvadaf ypgdlqppra
      541 lfryhyylne lav
//
LOCUS       XP_077501497             802 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 2-like [Amblyomma americanum].
ACCESSION   XP_077501497
VERSION     XP_077501497.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645371.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526885) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..802
                     /product="endothelin-converting enzyme 2-like"
                     /calculated_mol_wt=87722
     Region          93..726
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..802
                     /gene="LOC144112562"
                     /coded_by="XM_077645371.1:1..2409"
                     /db_xref="GeneID:144112562"
ORIGIN      
        1 magegckaps ttsggldrrq ravalghlls sllgvaltls aitiglsvlr hqrgsegsgv
       61 fgmgrsswnt cnsklcmahf rrlkdslnrs tapcgdfyrf acgawhpraa ksetvfrdll
      121 avaqqdairy leavndasnk mvlptgkvll pdsrpvlrin raerahqvcl hsgsstkdaa
      181 lltefladrs lswperttap aqpldvlldl dinwnlglwf svrvspipgc nqrqvrvgsg
      241 ivsydwkral dtmvtrgvyk arvrrfhdal rarsfaamsd kemkelrrye stivgalcgs
      301 vsggqnelaf plkqmqnlat prlaaaqwdt llnwqlnkal rvteadrvia tsvkllqavd
      361 yllsslpaev vlhqlawslv qmigwmadsa lpgrptayem edmrsadcfw vthrafgvth
      421 lsgyinttyp lhwlssvdai lmtitqtain fflktswiha esraaavdkl rrmntslwps
      481 dsylnaseva ailenfpepr edgavlrywl dalsarrall grqwdvadaf ypgdlqppra
      541 lfryhyyrne lavslhalrs plfvegvgla vnmgglgahy avalarafdp rgvlldgrgs
      601 tsglwwgkss yrdyerrtac pataasgaeg gvqafegvaa ieiaygafna srsiadqgtr
      661 thqrrriflg lsedlaffva fceascargp serqqlsctl plknfrefat aftcpvaspm
      721 npavrcgffd asdqraarkn ggesedagks astnasanvg adgavtswtr pparrsgsdd
      781 qdpltlatrg laghmvrqpp vl
//
LOCUS       XP_077501498             802 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 2-like [Amblyomma americanum].
ACCESSION   XP_077501498
VERSION     XP_077501498.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645372.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526885) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..802
                     /product="endothelin-converting enzyme 2-like"
                     /calculated_mol_wt=87914
     Region          93..726
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..802
                     /gene="LOC144112563"
                     /coded_by="XM_077645372.1:1..2409"
                     /db_xref="GeneID:144112563"
ORIGIN      
        1 magegckaps ttsggldrrq ravalghlls sllgvaltls aitiglsvlr hqrgsegsgv
       61 fgmgrsswnt cnsklcmahf rrlkdslnrs tapcgdfyrf acgawhpraa ksetvfrdll
      121 avaqqdairy leavnddsnk mvlptgkvll pdsrpvlrin raerahqvcl hsgsstkdaa
      181 lltefladrs lswperttap aqpldvlldl dinwnlglwf svrvspipgc nqrqvrvgsg
      241 ivsydwkral dtmvtrgvyk arvrrfhdal rarsfaamsd kgmkelrrye stivralcgs
      301 vsggqnelaf plkqmqnlat prlaaaqwdt llnwqlnkal rvteadrvia tsvkllqavd
      361 yllsslpaev vlhqlawslv qmigwmadsa lpgrptayem edmrsadcfw vthrafgvth
      421 lsgyinttyp lhwlssvdai lmtitqtain fflktswiha esraaavdkl rrmntslwps
      481 dsylnaseva ailenfpepr edgavlrywl dalsarrall grqwdvadaf ypgdlqppra
      541 lfryhyyrne lavslhalrs plfvegvgla vnmgglgahy avalarafdp rgvlldgrgs
      601 tsglwwgkss yrdyerrtac pataasgaeg gvqafegvaa ieiaygafna srsiadqgtr
      661 thqrrriflg lsedqaffva fceascargp serqqlsctl plknfrefat aftcpvaspm
      721 npalrcgffe asdqraarkn ggesedagks astnasanvg adgavtswtr pparrsgsdd
      781 qdpwtlmttg laghmvrqpp vl
//
LOCUS       XP_077501499             802 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 2-like [Amblyomma americanum].
ACCESSION   XP_077501499
VERSION     XP_077501499.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645373.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526885) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..802
                     /product="endothelin-converting enzyme 2-like"
                     /calculated_mol_wt=87795
     Region          93..726
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..802
                     /gene="LOC144112564"
                     /coded_by="XM_077645373.1:1..2409"
                     /db_xref="GeneID:144112564"
ORIGIN      
        1 magegckaps ttsggldrrq ravalghlls sllgvaltls aitiglsvlr hqrgsegsgv
       61 fgmgrsswnt cnsklcmahf rrlkdslnrs tapcgdfyrf acgawhpraa ksetvfrdll
      121 avaqqdairy leavndasnk mvlptgkvll pdsrpvlrin raerahqvcl hsgsstkdaa
      181 lltefladrs lswperttap aqpldvlldl dinwnlglwf svrvspipgc nqrqvrvgsg
      241 ivsydwkral dtmvtrgvyk arvrrfhdal rarsfaamsd kgmkelrrye stivralcgs
      301 vsggqnelaf plkqmqnlat prlaaaqwdt llnwqlnkal rvteadrvia tsvkllqavd
      361 yllsslpaev vlhqlawslv hmigwmadsa lpgrptayem edmrsadcfw vthrafgvth
      421 lsgyinttyp lhwlssvdai lmtitqtain fflktswiha esraaavdkl rrmntslwps
      481 dsylnaseva ailenfpepr edgavlrywl dalsarrall grqwdvadaf ypgdlqppra
      541 lfryhyyrne lavslhalrs plfvegvgla vnmgglgahy avalarvfdp rgvlldgrgs
      601 tsglwwgkss yrdyerrtac pataasgaeg gvqafegvaa ieiaygafna srsiadqgtr
      661 thqrrriflg lsedqaffva fceascargp serqqlsctl plknfrefat aitcpvaspm
      721 npalrcgffe asdqraarkn ggesedagks astnasanvg adgavtswtr pparrsgsdd
      781 qdpltlatrg laghmvrqpp vl
//
LOCUS       XP_077501500             802 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 2-like [Amblyomma americanum].
ACCESSION   XP_077501500
VERSION     XP_077501500.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645374.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526885) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..802
                     /product="endothelin-converting enzyme 2-like"
                     /calculated_mol_wt=87914
     Region          93..726
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..802
                     /gene="LOC144112565"
                     /coded_by="XM_077645374.1:1..2409"
                     /db_xref="GeneID:144112565"
ORIGIN      
        1 magegckaps ttsggldrrq ravalghlls sllgvaltls aitiglsvlr hqrgsegsgv
       61 fgmgrsswnt cnsklcmahf rrlkdslnrs tapcgdfyrf acgawhpraa ksetvfrdll
      121 avaqqdairy leavnddsnk mvlptgkvll pdsrpvlrin raerahqvcl hsgsstkdaa
      181 lltefladrs lswperttap aqpldvlldl dinwnlglwf svrvspipgc nqrqvrvgsg
      241 ivsydwkral dtmvtrgvyk arvrrfhdal rarsfaamsd kgmkelrrye stivralcgs
      301 vsggqnelaf plkqmqnlat prlaaaqwdt llnwqlnkal rvteadrvia tsvkllqavd
      361 yllsslpaev vlhqlawslv qmigwmadsa lpgrptayem edmrsadcfw vthrafgvth
      421 lsgyinttyp lhwlssvdai lmtitqtain fflktswiha esraaavdkl rrmntslwps
      481 dsylnaseva ailenfpepr edgavlrywl dalsarrall grqwdvadaf ypgdlqppra
      541 lfryhyyrne lavslhalrs plfvegvgla vnmgglgahy avalarafdp rgvlldgrgs
      601 tsglwwgkss yrdyerrtac pataasgaeg gvqafegvaa ieiaygafna srsiadqgtr
      661 thqrrriflg lsedqaffva fceascargp serqqlsctl plknfrefat aftcpvaspm
      721 npalrcgffe asdqraarkn ggesedagks astnasanvg adgavtswtr pparrsgsdd
      781 qdpwtlmttg laghmvrqpp vl
//
LOCUS       XP_077501502             452 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112569 [Amblyomma americanum].
ACCESSION   XP_077501502
VERSION     XP_077501502.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645376.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526890) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 45% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..452
                     /product="uncharacterized protein LOC144112569"
                     /calculated_mol_wt=50622
     Region          78..>135
                     /region_name="SWIM"
                     /note="Uncharacterized protein, contains SWIM-type Zn
                     finger domain [Function unknown]; COG4715"
                     /db_xref="CDD:443750"
     Region          250..439
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(302,336,347,349)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..452
                     /gene="LOC144112569"
                     /coded_by="XM_077645376.1:1..1359"
                     /db_xref="GeneID:144112569"
ORIGIN      
        1 mdagwvddtk lwpklqrpfv ynymvempdt dgkltvnyrg faegsnllds ghvgvalyqq
       61 kngvkcikan irssqtvsrt yqtqinfgqp fsasctckag lsercshica llmkvveats
      121 kgltgqsstd kacawnaata knvtpatmev vychkktqsk tqqflsekem llhfekcpld
      181 vsklqntmlh tvlkprrkpp aggdedgraf lqqehgqhdc krlcqlcqdf fvtnvllspe
      241 qaatlanhpq gsaawhkerf lritasears ipikaapekw vskhihpsfy gngstrygre
      301 seakarawyk ktkttdvvec gliirpatsw lgaspdgiip dsdtvleikc ptpltvqkhg
      361 sldalfksgr ydvikkenkl tlskagknry ylqvqlqlfc cersrcdfvi wapneayiie
      421 vpydkefisk qvahledfyf shllpavvds kq
//
LOCUS       XP_077501503             170 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112570 [Amblyomma americanum].
ACCESSION   XP_077501503
VERSION     XP_077501503.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645377.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526890) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..170
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..170
                     /product="uncharacterized protein LOC144112570"
                     /calculated_mol_wt=19149
     Region          <48..106
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..170
                     /gene="LOC144112570"
                     /coded_by="XM_077645377.1:54..566"
                     /db_xref="GeneID:144112570"
ORIGIN      
        1 mwksrgktcs vfdckncdad lkvwnvsvce thgpqlhedc pctrpfamhr fpqgeknklv
       61 rqhwianlqr kdfnpgssar vcsvhfidgr pskdnpyptl hlgtptahhk grrklvrhdv
      121 kplepetcst aeplshhlsd eqdeesaaad dhlpiniate dvshwygnvv
//
LOCUS       XP_077501505             137 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112578 [Amblyomma americanum].
ACCESSION   XP_077501505
VERSION     XP_077501505.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645379.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526896) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..137
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..137
                     /product="uncharacterized protein LOC144112578"
                     /calculated_mol_wt=15286
     Region          58..78
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(63,65,67,69..70,73..74,77,91,93,97..98,101..102,105,
                     119,121,123,125..126,129..130,133)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          83..>137
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          86..106
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          98..123
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          114..133
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..137
                     /gene="LOC144112578"
                     /coded_by="XM_077645379.1:132..545"
                     /db_xref="GeneID:144112578"
ORIGIN      
        1 mlgatllevp crnlrqvvrl kallsssphq ecrdekciel hnpwqraeal sctgavrcdl
       61 cgfpsanrpa mlahqrlhvg rlplscvfcp rvftqkphlr dhvrthtger pfvcpvccag
      121 faqksvltrh srtthks
//
LOCUS       XP_077501506             124 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-scruin-like [Amblyomma americanum].
ACCESSION   XP_077501506
VERSION     XP_077501506.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645380.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526896) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 40% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..124
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..124
                     /product="alpha-scruin-like"
                     /calculated_mol_wt=13609
     Region          <52..>107
                     /region_name="NanM"
                     /note="N-acetylneuraminic acid mutarotase [Cell
                     wall/membrane/envelope biogenesis]; COG3055"
                     /db_xref="CDD:442289"
     CDS             1..124
                     /gene="LOC144112579"
                     /coded_by="XM_077645380.1:1..375"
                     /db_xref="GeneID:144112579"
ORIGIN      
        1 mdpnlsmvgr mdgsflrtks alglraapew qsvglrelqw trqpcelnfp villvggldp
       61 rnllgsgsav lkyhpfkdrw mlvgamiepr nyhaavyind klfvteggil tgaspresil
      121 tlis
//
LOCUS       XP_077501507             252 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112581 [Amblyomma americanum].
ACCESSION   XP_077501507
VERSION     XP_077501507.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645381.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526898) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 31% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..252
                     /product="uncharacterized protein LOC144112581"
                     /calculated_mol_wt=28036
     CDS             1..252
                     /gene="LOC144112581"
                     /coded_by="XM_077645381.1:1..759"
                     /db_xref="GeneID:144112581"
ORIGIN      
        1 miiitlccsk wqlaeiigdc vlptrpircg vviffqadgt askttqpitk teepakgivv
       61 gydserpvev dvsdlknnpa assadspgav ssvisdqlri iesrcndaen rlrrcnllff
      121 glsddisetw psaetkvidl ckkelditvd ssqierahri gkydpekcrp vmvkftlfkd
      181 kqqiigngik lkgtnyavre dfsfqtrlar rkliefakpq kvpyklnvdk lrindsvyvy
      241 daatdtvkls sa
//
LOCUS       XP_077501508             108 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112583 [Amblyomma americanum].
ACCESSION   XP_077501508
VERSION     XP_077501508.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645382.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526898) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..108
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..108
                     /product="uncharacterized protein LOC144112583"
                     /calculated_mol_wt=12400
     Region          <57..104
                     /region_name="SVWC"
                     /note="Single domain von Willebrand factor type C;
                     pfam15430"
                     /db_xref="CDD:464713"
     CDS             1..108
                     /gene="LOC144112583"
                     /coded_by="XM_077645382.1:134..460"
                     /db_xref="GeneID:144112583"
ORIGIN      
        1 mvklvavlfl fgadlalsgk dyngardqsl hvqvddsvke cwyrgynltk nipkhmetpc
       61 erwtcryknh yprvvvdgcd pvifseryve evsqnpenvf pkccgrkk
//
LOCUS       XP_077501510             177 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112588 [Amblyomma americanum].
ACCESSION   XP_077501510
VERSION     XP_077501510.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645384.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526898) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..177
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..177
                     /product="uncharacterized protein LOC144112588"
                     /calculated_mol_wt=20037
     CDS             1..177
                     /gene="LOC144112588"
                     /coded_by="XM_077645384.1:1..534"
                     /db_xref="GeneID:144112588"
ORIGIN      
        1 miamyvepdr rnrnaalpfv tfvynsalqr ttgyspfflv fgrshttlld acffsapass
       61 spslheqcls rvargrhlar lnteacnedr kshydtthhv vlfnpgdevl lstrlrtpgl
      121 cdkfqprfig pslvltqtsp vnyrvtpvdp ppdrryrgte ivhvyrlkpf irrsasd
//
LOCUS       XP_077501512             431 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112591 [Amblyomma americanum].
ACCESSION   XP_077501512
VERSION     XP_077501512.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645386.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526899) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..431
                     /product="uncharacterized protein LOC144112591"
                     /calculated_mol_wt=49103
     Region          166..>225
                     /region_name="Lebercilin"
                     /note="Ciliary protein causing Leber congenital amaurosis
                     disease; pfam15619"
                     /db_xref="CDD:464776"
     CDS             1..431
                     /gene="LOC144112591"
                     /coded_by="XM_077645386.1:93..1388"
                     /db_xref="GeneID:144112591"
ORIGIN      
        1 mrgyhhhqya ttfvshprpa flppdrpsrs arfccqryvg avvrsssfgv vrviamfayv
       61 rfrdddmktv lpisrfrsfq pkglkdfatn kwyevfwedn eqsgyyqaqi vrlfdtkeea
      121 erqkripvpp rpsdteteet tpssddylre vmrkaaaekk kkknnsqril rekqlqqlme
      181 nepdeqahel eqlrrenkdl rerlktmeka lcskifdaeq lmsrhcsckq krpeirtser
      241 vpakgaverm palsappgpe kvppaapeak padpparesq rpvavlappq pslghnpatp
      301 eecaadqleg lpfqatgskv flghgvsltr egfnhlmsrq kdsvfvreac vqifstaglv
      361 gksitgnmcn rtkadpkppl dqqkyaalsa ffqhyleary eadearrrhr slnkivagkl
      421 advmkmqrrd v
//
LOCUS       XP_077501513             210 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112592 [Amblyomma americanum].
ACCESSION   XP_077501513
VERSION     XP_077501513.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645387.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526899) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..210
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..210
                     /product="uncharacterized protein LOC144112592"
                     /calculated_mol_wt=23865
     Region          <26..205
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          47..67
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(52,54,56,58..59,62..63,66,80,82,86..87,90..91,94,
                     108,110,112,114..115,118..119,122)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          75..95
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          103..123
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          131..150
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          159..179
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          187..204
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..210
                     /gene="LOC144112592"
                     /coded_by="XM_077645387.1:397..1029"
                     /db_xref="GeneID:144112592"
ORIGIN      
        1 madqnvgvfp geippdekcp vnysvpfist smsthrdkpt gkqihqcthc gnifkqrshl
       61 vehirthagd rpyrcsycds afvsrgslvr hlrthtgdrp yrcdhcgstf siksnldrhq
      121 rthtgdrpyr cdhcgstfve mghlvthvri qtgekpfqcq lcpmafaqkg slvahvrshk
      181 gekpfqcdlc skafsvkwkl khhrdktlke
//
LOCUS       XP_077501514             210 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112592 [Amblyomma americanum].
ACCESSION   XP_077501514
VERSION     XP_077501514.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645388.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526899) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..210
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..210
                     /product="uncharacterized protein LOC144112592"
                     /calculated_mol_wt=23865
     Region          <26..205
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          47..67
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(52,54,56,58..59,62..63,66,80,82,86..87,90..91,94,
                     108,110,112,114..115,118..119,122)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          75..95
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          103..123
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          131..150
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          159..179
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          187..204
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..210
                     /gene="LOC144112592"
                     /coded_by="XM_077645388.1:589..1221"
                     /db_xref="GeneID:144112592"
ORIGIN      
        1 madqnvgvfp geippdekcp vnysvpfist smsthrdkpt gkqihqcthc gnifkqrshl
       61 vehirthagd rpyrcsycds afvsrgslvr hlrthtgdrp yrcdhcgstf siksnldrhq
      121 rthtgdrpyr cdhcgstfve mghlvthvri qtgekpfqcq lcpmafaqkg slvahvrshk
      181 gekpfqcdlc skafsvkwkl khhrdktlke
//
LOCUS       XP_077501516             424 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112595 [Amblyomma americanum].
ACCESSION   XP_077501516
VERSION     XP_077501516.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645390.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526899) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..424
                     /product="uncharacterized protein LOC144112595"
                     /calculated_mol_wt=47177
     CDS             1..424
                     /gene="LOC144112595"
                     /coded_by="XM_077645390.1:47..1321"
                     /db_xref="GeneID:144112595"
ORIGIN      
        1 mlfkydfrvg sgtikssyir qayekdremq irsipklsar hfnltfaskm svklaaqvfs
       61 nhcaaalctl vtfqqlpsea ihtarfveri drlfdslnts qkrgkspyas aicagsvhee
      121 fleeciavfe nievlgcarq plcirgfcqt travlllwnh lasrygliyl ltrhlnqdal
      181 entfaivrsk sgsnsksscr efqaafrhll vsnlfklses sncaedmssl latlpvclsk
      241 sapslctaat slpvavevlp ydyqspilen nivyfagwla ykfmndhrcv tgphtcevkl
      301 enaafadqsq vllylsvknp gegdfgslsv ptpsfvafin aceqlfvass snivgkeqvg
      361 hdlcmllhak veksltvcgd dvydglfalf vrvrlhwlar rknlelhsqt tkrkatkqal
      421 rlsk
//
LOCUS       XP_077501517             108 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112596 [Amblyomma americanum].
ACCESSION   XP_077501517
VERSION     XP_077501517.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645391.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526899) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..108
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..108
                     /product="uncharacterized protein LOC144112596"
                     /calculated_mol_wt=12102
     CDS             1..108
                     /gene="LOC144112596"
                     /coded_by="XM_077645391.1:165..491"
                     /db_xref="GeneID:144112596"
ORIGIN      
        1 mslrhvmkrc svvhqgytat awivplvsry tdsrwtftga ergrssaats awrrrrrrsy
       61 ensgfargts nhlcicrqeg cdtmvclleq alltpqanaa atwtvhnl
//
LOCUS       XP_077501518             108 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112596 [Amblyomma americanum].
ACCESSION   XP_077501518
VERSION     XP_077501518.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645392.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526899) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..108
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..108
                     /product="uncharacterized protein LOC144112596"
                     /calculated_mol_wt=12102
     CDS             1..108
                     /gene="LOC144112596"
                     /coded_by="XM_077645392.1:165..491"
                     /db_xref="GeneID:144112596"
ORIGIN      
        1 mslrhvmkrc svvhqgytat awivplvsry tdsrwtftga ergrssaats awrrrrrrsy
       61 ensgfargts nhlcicrqeg cdtmvclleq alltpqanaa atwtvhnl
//
LOCUS       XP_077501519             238 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112597 [Amblyomma americanum].
ACCESSION   XP_077501519
VERSION     XP_077501519.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645393.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526899) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..238
                     /product="uncharacterized protein LOC144112597"
                     /calculated_mol_wt=26758
     Region          <24..193
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          49..69
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(54,56,58,60..61,64..65,68,82,84,88..89,92..93,96,
                     110,112,114,116..117,120..121,124)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          77..97
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          105..125
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          133..153
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(138,140,142,144..145,148..149,152,166,168,172..173,
                     176..177,180,194,196,198,200..201,204..205,208)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          161..181
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          189..209
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          201..225
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          217..236
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..238
                     /gene="LOC144112597"
                     /coded_by="XM_077645393.1:1821..2537"
                     /db_xref="GeneID:144112597"
ORIGIN      
        1 mattdckagv ilgqnlphqk cpvnhcvpti stsmsnhrdk ptgkqaqqct hcgnilkkrs
       61 dlaahvctht gdrpyhccyc dstfayksyl vrhlrthtgd rpyccdhcgs tfaaksslar
      121 hqrmhtgdrp ypcdhcdskf alkssldnhv rthtgdrpyr cdhcgnafvq kihlvqhvri
      181 htgekpfqcq lcpmafaqna alvvhmrshk gekpfqcdlc tkafsvkcsl khhketrh
//
LOCUS       XP_077501520             236 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112598 [Amblyomma americanum].
ACCESSION   XP_077501520
VERSION     XP_077501520.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645394.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526899) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..236
                     /product="uncharacterized protein LOC144112598"
                     /calculated_mol_wt=26992
     Region          <44..191
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          47..67
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          75..95
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(80,82,84,86..87,90..91,94,108,110,114..115,118..119,
                     122,136,138,140,142..143,146..147,150)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          103..123
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          131..151
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          159..179
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          187..207
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          215..234
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..236
                     /gene="LOC144112598"
                     /coded_by="XM_077645394.1:110..820"
                     /db_xref="GeneID:144112598"
ORIGIN      
        1 madqnvgvfp geippdekcp vnysvpfist smrthrdkpt gkqihqcthc gnifkqrshl
       61 vehirthagd rpyrcsycds afvsrgslvr hlrthtgdrp yrcdhcgstf siksnldrhq
      121 rthtgdrpyr cdhcgstfsi ksnldrhqrt htgdrpyrcd hcgstfvekg hlvthvriht
      181 gekpfqcqlc pmafaqkgsl vahvrshkge kpfqcdlcsk afsvkwklkh hketrh
//
LOCUS       XP_077501521             238 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112599 [Amblyomma americanum].
ACCESSION   XP_077501521
VERSION     XP_077501521.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645395.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526899) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..238
                     /product="uncharacterized protein LOC144112599"
                     /calculated_mol_wt=26795
     Region          <24..193
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          49..69
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          77..97
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          105..125
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          133..153
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(138,140,142,144..145,148..149,152,166,168,172..173,
                     176..177,180,194,196,198,200..201,204..205,208)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          161..181
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          189..209
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          201..225
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          217..236
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..238
                     /gene="LOC144112599"
                     /coded_by="XM_077645395.1:101..817"
                     /db_xref="GeneID:144112599"
ORIGIN      
        1 mattdckagv ilgqnlphqk cpvnhcvpti stsmsnhrdk ptgkqahqct hcgnilkkra
       61 dlaahvctht gdrpyhccyc dstfayksyl vrhlrthtgd rpyccdhcgs tfaakssldr
      121 hqrmhtgdrp ypcdhcdskf alkssldnhv rthtgdrpyr cdhcgnafvq kihlvqhvri
      181 htgekpfqcq lcpmafaqna alvvhmrshk gekpfqcdlc tkafsvkcsl khhketrh
//
LOCUS       XP_077501523             180 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112600 [Amblyomma americanum].
ACCESSION   XP_077501523
VERSION     XP_077501523.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645397.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526899) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..180
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..180
                     /product="uncharacterized protein LOC144112600"
                     /calculated_mol_wt=20328
     Region          47..67
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(52,54,56,58..59,62..63,66,80,82,86..87,90..91,94,
                     108,110,112,114..115,118..119,122)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          75..95
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          87..111
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          103..123
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          115..140
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          131..151
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          159..178
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..180
                     /gene="LOC144112600"
                     /coded_by="XM_077645397.1:6..548"
                     /db_xref="GeneID:144112600"
ORIGIN      
        1 madhnfgvfp gqsppdekcl lnhsvpstst svnthrdkpt gkqihqcthc gkifqkrsdl
       61 vahirthtgd rpyhcsdcds tfayknslvr hlrthtgdrp yrcdhcgsaf vekghlvthv
      121 rihtgekpfq cqlcpmafar kgtlvahvrs hkgekpfqcd scskafsvkw klkhhketrh
//
LOCUS       XP_077501524             363 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112601 [Amblyomma americanum].
ACCESSION   XP_077501524
VERSION     XP_077501524.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645398.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526899) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..363
                     /product="uncharacterized protein LOC144112601"
                     /calculated_mol_wt=40723
     Region          27..>310
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          47..67
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(52,54,56,58..59,62..63,66,80,82,86..87,90..91,94,
                     108,110,112,114..115,118..119,122)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          75..95
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          103..123
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          131..151
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          159..179
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          254..274
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(259,261,263,265..266,269..270,273,287,289,293..294,
                     297..298,301,315,317,319,321..322,325..326,329)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          282..302
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          310..330
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          322..347
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     CDS             1..363
                     /gene="LOC144112601"
                     /coded_by="XM_077645398.1:73..1164"
                     /db_xref="GeneID:144112601"
ORIGIN      
        1 madhnvgvfp geippdekcp vnysvpfist smsthrdkpt gkqihqcthc gnifkqrshl
       61 vehirthagd rpyrcsycds afvsrgslvr hlrthtgdrp yrcdhcgstf siksnldrhq
      121 rmhtgdrpyr cdhcgsafve kghlvthvri htgekpfqcq lcpmafaqka slvahvrshk
      181 gekplfsldp waaegpcvmv npsgtsvmad hnfgvfpgqs ppdekcllnh svpststsvn
      241 thrdkptgkq ihqctpcgnv fkkrshlveh irthtgdrpy hcsdcdstfa yknslvrhlr
      301 thtgdrpyrc dhcgnafver hhlvkhvrih tgekpfqcql cpmafaqkvl pgslgsrrsl
      361 rdg
//
LOCUS       XP_077501525             119 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger protein 764-like [Amblyomma americanum].
ACCESSION   XP_077501525
VERSION     XP_077501525.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645399.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526899) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 15% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..119
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..119
                     /product="zinc finger protein 764-like"
                     /calculated_mol_wt=13561
     Region          47..67
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(52,54,56,58..59,62..63,66,80,82,86..87,89,103,105,
                     107,109..110,113..114,117)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          59..84
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          98..117
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..119
                     /gene="LOC144112602"
                     /coded_by="XM_077645399.1:17..376"
                     /db_xref="GeneID:144112602"
ORIGIN      
        1 madhnfgvfp gqsppdeqrl lnhsvpstrt svnthrdkpt gkqihqctpc gnvfkkrshl
       61 vehirthtgd rpyhcsdcds tfaykdsrsh kgekpfqcds cskafsvkwk lkhhketrh
//
LOCUS       XP_077501526             190 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112603 [Amblyomma americanum].
ACCESSION   XP_077501526
VERSION     XP_077501526.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645400.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526899) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..190
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..190
                     /product="uncharacterized protein LOC144112603"
                     /calculated_mol_wt=21461
     Region          <12..159
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          47..67
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(52,54,56,58..59,62..63,66,80,82,86..87,90..91,94,
                     108,110,112,114..115,118..119,122)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          75..95
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          87..111
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          103..123
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          131..151
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          143..168
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          159..176
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..190
                     /gene="LOC144112603"
                     /coded_by="XM_077645400.1:3..575"
                     /db_xref="GeneID:144112603"
ORIGIN      
        1 madrnfgvfl gqsppdeqrl lnhsvpstst svnthwdkpt gkqihqcthc gnvfkqrsyl
       61 vkhirthtgd rpyhcsdcds tfaykdslvr hlrthtgdrp yrcdhcdskf svksslhnhl
      121 rthtgdrpyc cdhcgsafve rshlvkhvri htgekpyqcq mcpmafaqkg tlvahvlpgs
      181 lgsrrslrdg
//
LOCUS       XP_077501527             294 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112604 [Amblyomma americanum].
ACCESSION   XP_077501527
VERSION     XP_077501527.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645401.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526899) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 50% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..294
                     /product="uncharacterized protein LOC144112604"
                     /calculated_mol_wt=32368
     Region          44..>64
                     /region_name="PHA00616"
                     /note="hypothetical protein"
                     /db_xref="CDD:177288"
     Region          47..67
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          59..84
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          75..95
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(80,82,84,86..87,90..91,94,108,110,114..115,118..119,
                     122,136,138,140,142..143,146..147,150)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          87..111
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          103..123
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          115..140
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          131..151
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..294
                     /gene="LOC144112604"
                     /coded_by="XM_077645401.1:102..986"
                     /db_xref="GeneID:144112604"
ORIGIN      
        1 madhnfglfp gqsppdekrl fnhsvpstst slnthrdkpt gkqiyqcthc gnifkkrsyl
       61 vehirthtgd rpyhcsdcds tfayknslvr hlrthtsdrp yrcdhcdrkf svkssldnhl
      121 rthtgdrpyr cdhcdsayvq kshlvdhvri htapkgisgn stkaadepas qpptvdcprf
      181 anetsgngac ptepedveak epsediheks garvtsvtpp vtttakrphd qtspegdkvv
      241 apgveeppak taqsrrpklr prpnlsddkr aggkvlheqa dvlppddtgg hsgv
//
LOCUS       XP_077501528             212 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112605 [Amblyomma americanum].
ACCESSION   XP_077501528
VERSION     XP_077501528.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645402.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526899) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 30% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..212
                     /product="uncharacterized protein LOC144112605"
                     /calculated_mol_wt=23876
     Region          27..>109
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          47..67
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(52,54,56,58..59,62..63,66,80,82,86..87,90..91,94,
                     108,110,112,114..115,118..119,122)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          75..95
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          103..123
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          115..139
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          131..151
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          143..168
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          159..175
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..212
                     /gene="LOC144112605"
                     /coded_by="XM_077645402.1:73..711"
                     /db_xref="GeneID:144112605"
ORIGIN      
        1 madhnvgvfp geippdekcp vnysvpfist smsthrdkpt gkqihqcthc gnifkqrshl
       61 vehirthagd rpyrcsycds afvsrgslvr hlrthtgdrp yrcdhcgstf siksnldrhq
      121 rmhtgdrpyr cdhcgsafve kghlvthvri htgekpfqcq lcpmafaqka slvahsigrv
      181 sgvrsrlrql rfyggkalrr lcgvrspppp rk
//
LOCUS       XP_077501530             356 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112606 [Amblyomma americanum].
ACCESSION   XP_077501530
VERSION     XP_077501530.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645404.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526899) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 28% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..356
                     /product="uncharacterized protein LOC144112606"
                     /calculated_mol_wt=40598
     Region          47..80
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(52,54,56,58..59,75..76,79,93,95,99..100,103..104,
                     107,121,123,125,127..128,131..132,135)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          72..96
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          88..108
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          100..124
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          116..135
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          173..>255
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          193..213
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(198,200,202,204..205,208..209,212,226,228,232..233,
                     236..237,240,254,256,258,260..261,264..265,268)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          221..241
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          249..269
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          261..285
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          277..296
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          305..325
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          333..350
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..356
                     /gene="LOC144112606"
                     /coded_by="XM_077645404.1:1..1071"
                     /db_xref="GeneID:144112606"
ORIGIN      
        1 madhnfavfp ghsppdekrp vkhsepsist smsthrdkst gkqthqcthc gnvfkkrshl
       61 vehirthtvk ssldrhqrth tgdrpyrcdh cdrkfsvkss ldnhvrthtg drpyrcdhcg
      121 safvershlv khvriamelf hvsgsvmadq nvgvfpgeip pdekcpvnys vpfistsmst
      181 hrdkptgkqi hqcthcgnif kqrshlvehi rthagdrpyr csycdsafvs rgslvrhlrt
      241 htgdrpyrcd hcgstfsiks nldrhqrtht gdrpyrcdhc gstfvemghl vthvriqtge
      301 kpfqcqlcpm afaqkgslva hvrshkgekp fqcdlcskaf svkwklkhhr dktlke
//
LOCUS       XP_077501531             206 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112607 [Amblyomma americanum].
ACCESSION   XP_077501531
VERSION     XP_077501531.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645405.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526899) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..206
                     /product="uncharacterized protein LOC144112607"
                     /calculated_mol_wt=23431
     Region          <44..191
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          47..67
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          75..95
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          103..123
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(108,110,112,114..115,118..119,122,136,138,142..143,
                     146..147,150,164,166,168,170..171,174..175,178)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          131..151
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          159..179
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          187..203
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..206
                     /gene="LOC144112607"
                     /coded_by="XM_077645405.1:112..732"
                     /db_xref="GeneID:144112607"
ORIGIN      
        1 madhnfgvfp gqsppdekrl lnhsvpstst svnthrdkpt gkqihqcthc gnvfkkrsyl
       61 vehirthtgd rpyhcsdcds tfayknslvr hlrthtddrp yccdhcgstf svkssldrhq
      121 rthtgdrpyr cdhcdrkfsv kssldnhlrt htgdrpyrcd hcgsafvers hlvkhiriht
      181 gekpyqcqlc pmafalkgtl vahssp
//
LOCUS       XP_077501532              93 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112608 [Amblyomma americanum].
ACCESSION   XP_077501532
VERSION     XP_077501532.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645406.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526899) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 41% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..93
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..93
                     /product="uncharacterized protein LOC144112608"
                     /calculated_mol_wt=10809
     Region          14..38
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          16..36
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(21,23,25,27..28,31..32,35,50..51,54..55,58,72,74,76,
                     78..79,82..83,86)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          39..>93
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          51..76
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          67..87
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..93
                     /gene="LOC144112608"
                     /coded_by="XM_077645406.1:1..282"
                     /db_xref="GeneID:144112608"
ORIGIN      
        1 migvlrthtg drpyrcdhcd rkfsvkssld nhvrthtgdr pyrcdhctrn slvrhlrtht
       61 gdrpyrcsfc dsafsrkdnl vrhvcihtge kpf
//
LOCUS       XP_077501533             256 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112609 [Amblyomma americanum].
ACCESSION   XP_077501533
VERSION     XP_077501533.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645407.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526899) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 14% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..256
                     /product="uncharacterized protein LOC144112609"
                     /calculated_mol_wt=29220
     Region          <45..211
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          47..67
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          75..95
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          123..143
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(128,130,132,134..135,138..139,142,156,158,162..163,
                     166..167,170,184,186,188,190..191,194..195,198)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          135..159
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          151..171
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          179..199
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          191..216
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          207..227
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          235..254
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..256
                     /gene="LOC144112609"
                     /coded_by="XM_077645407.1:3..773"
                     /db_xref="GeneID:144112609"
ORIGIN      
        1 madhnfgvfp ghsppdekrp vkhsepsist smsthrdkst gkqthqcthc gnvfkkrshl
       61 vehirthtgd rpyhcsdcds tfaykdslvr hlrthtgdrp yccdhnslvr hlrthtgdrp
      121 yccehcgstf siksnldrhq rthtgdrpyr cdhcsnkfaa ksnldshlrt htgdrpyrcd
      181 hcvsafveks hlvthvriht gekpyqcqlc pmafaqkatl vahvrshkge kpfqcdscsk
      241 afsvksnlkh hketrh
//
LOCUS       XP_077501534             174 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112610 [Amblyomma americanum].
ACCESSION   XP_077501534
VERSION     XP_077501534.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645408.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526899) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..174
                     /product="uncharacterized protein LOC144112610"
                     /calculated_mol_wt=19627
     Region          47..97
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          109..129
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          121..146
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          137..157
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          149..173
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     CDS             1..174
                     /gene="LOC144112610"
                     /coded_by="XM_077645408.1:47..571"
                     /db_xref="GeneID:144112610"
ORIGIN      
        1 mgdhnvcmfp gqsppdekhp vnhsvpsist smsthqdkpt gkqihqcthc gkifqersdl
       61 vvmadhnfgv fpgqsppdeq rllnhsvpst stsvnthrdk ptgkqiyqct hcgnvfkkrs
      121 ylvqhirtht gdrpyhcsdc dstfaykdsl vrhlrthtgd rpyrcdhcds kftm
//
LOCUS       XP_077501535             169 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112612 [Amblyomma americanum].
ACCESSION   XP_077501535
VERSION     XP_077501535.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645409.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526899) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..169
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..169
                     /product="uncharacterized protein LOC144112612"
                     /calculated_mol_wt=19040
     CDS             1..169
                     /gene="LOC144112612"
                     /coded_by="XM_077645409.1:1..510"
                     /db_xref="GeneID:144112612"
ORIGIN      
        1 mtvyrgddpr eiashikdlv lllqenvasv vlvfkvlprh fdeprkaqfe drrrrqlnrr
       61 lsatlkrlsg vhilnpehrf ldasgkpmls lfaadryhva rdrgidqmsk iivaalvkiy
      121 gpgiasasra rpgevyvvhr crrcgakghk tdhcwaycsp rrhaaagrg
//
LOCUS       XP_077501537             208 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112613 [Amblyomma americanum].
ACCESSION   XP_077501537
VERSION     XP_077501537.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645411.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526899) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..208
                     /product="uncharacterized protein LOC144112613"
                     /calculated_mol_wt=24021
     Region          47..67
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          59..84
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          75..95
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          87..111
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          103..123
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(108,110,112,114..115,118..119,122,136,138,142..143,
                     146..147,150,164,166,168,170..171,174..175,178)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          131..151
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          143..168
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          159..179
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          187..206
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..208
                     /gene="LOC144112613"
                     /coded_by="XM_077645411.1:87..713"
                     /db_xref="GeneID:144112613"
ORIGIN      
        1 madhnfglfp gqsppdekrl fnhsvpstst slnthrdkpt gkqiyqcthc gnvfkkrsyl
       61 vehirthtgd rpyhcsdcds tfayknslvr hlrthtsdrp yrcdhcdrkf svkssldnhl
      121 rthtgdrpyr cdhcdsayvq kshlvdhvri htgekpfqcq lcpmafarkg tlvahvrshk
      181 gekpfqcdsc skafsvkwkl khhketrh
//
LOCUS       XP_077501538             292 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112614 [Amblyomma americanum].
ACCESSION   XP_077501538
VERSION     XP_077501538.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645412.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526899) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..292
                     /product="uncharacterized protein LOC144112614"
                     /calculated_mol_wt=32857
     CDS             1..292
                     /gene="LOC144112614"
                     /coded_by="XM_077645412.1:2..880"
                     /db_xref="GeneID:144112614"
ORIGIN      
        1 mhrtlkhsfl egkknhrvdr litalmdmty eslmkrvqhi vkakrtskms rieknhrsav
       61 aittpakrne dgtwtvqsqs trgltyrisk ekgsaccplk csecmvcvhs yscdceehrm
      121 sltickhihc vviagapegr dqkilkatea vssnskasea fhilesvqta eastsmtksd
      181 lvlakmellk eyvaeqeipq evhnkaskll dpvlkiyegt kkqemphtsr epankkiehq
      241 vrfhstkkqr lsssgtsfsk ptelqkenik kqilnsndea tviitspghn yi
//
LOCUS       XP_077501539             515 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112615 [Amblyomma americanum].
ACCESSION   XP_077501539
VERSION     XP_077501539.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645413.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526899) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..515
                     /product="uncharacterized protein LOC144112615"
                     /calculated_mol_wt=57327
     Region          4..97
                     /region_name="THAP"
                     /note="The THAP domain is a putative DNA-binding domain
                     (DBD) and probably also binds a zinc ion; smart00980"
                     /db_xref="CDD:214951"
     Region          239..291
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          321..489
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..515
                     /gene="LOC144112615"
                     /coded_by="XM_077645413.1:133..1680"
                     /db_xref="GeneID:144112615"
ORIGIN      
        1 mvlcaivgcs nrsdssrrkk kptntnfyri priiadkcer sktlstnrrt lwlsrikrad
       61 ldpdnpnfrv cgahfttgap adlfdntnpd wapslhlgys trpvgsdrhd rsqqrrqrkr
      121 caetemtgse epcgvaapvp dvppsphqae evdgageetg vyvqtdmgms digaleqeci
      181 rlndcvyttr sekvqlemte eslrssepkv vfytgiasft tllaifkmle pfvshsvnns
      241 lakfqefvlf lmkiklnlqn adlafrfnvs estvsrifdk wlhvahcrlk sqvmwpersa
      301 lqrtmpqafy dsfgsdvavi idcfeikier pssylprset wstykgsnta kyligiapqg
      361 ivtfisegwg grvsdkhite hcglldnllp gdvvladrgf nvadsvgfyr arlhlpaytk
      421 gkkqlsaaev gstrklanvr ihvervigli rnkflllksv lpidyvtcrq gdsitpldki
      481 vtvccalsnl cpsivaalke pskenvpesv dgcpp
//
LOCUS       XP_077501540             104 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112616 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501540
VERSION     XP_077501540.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645414.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526899) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..104
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..104
                     /product="uncharacterized protein LOC144112616 isoform X1"
                     /calculated_mol_wt=11418
     CDS             1..104
                     /gene="LOC144112616"
                     /coded_by="XM_077645414.1:78..392"
                     /db_xref="GeneID:144112616"
ORIGIN      
        1 marlvkrinl arrsqnapfa kfssslhvas lspfgaergr ssaatsawrr rrrrsyensg
       61 fargtsnhlc icrqegcdtm vclleqallt pqanaaatwt vhnl
//
LOCUS       XP_077501541             104 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112616 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501541
VERSION     XP_077501541.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645415.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526899) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..104
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..104
                     /product="uncharacterized protein LOC144112616 isoform X1"
                     /calculated_mol_wt=11418
     CDS             1..104
                     /gene="LOC144112616"
                     /coded_by="XM_077645415.1:78..392"
                     /db_xref="GeneID:144112616"
ORIGIN      
        1 marlvkrinl arrsqnapfa kfssslhvas lspfgaergr ssaatsawrr rrrrsyensg
       61 fargtsnhlc icrqegcdtm vclleqallt pqanaaatwt vhnl
//
LOCUS       XP_077501542              99 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112616 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501542
VERSION     XP_077501542.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645416.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526899) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..99
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..99
                     /product="uncharacterized protein LOC144112616 isoform X2"
                     /calculated_mol_wt=11064
     CDS             1..99
                     /gene="LOC144112616"
                     /coded_by="XM_077645416.1:157..456"
                     /db_xref="GeneID:144112616"
ORIGIN      
        1 mlrgtpryta tawivplvsr ytdsrwtftg aergrssaat sawrrrrrrs yensgfargt
       61 snhlcicrqe gcdtmvclle qalltpqana aatwtvhnl
//
LOCUS       XP_077501543             207 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112619 [Amblyomma americanum].
ACCESSION   XP_077501543
VERSION     XP_077501543.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645417.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526901) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..207
                     /product="uncharacterized protein LOC144112619"
                     /calculated_mol_wt=23787
     Region          60..203
                     /region_name="Baculo_FP"
                     /note="Baculovirus FP protein; pfam03258"
                     /db_xref="CDD:367421"
     CDS             1..207
                     /gene="LOC144112619"
                     /coded_by="XM_077645417.1:1..624"
                     /db_xref="GeneID:144112619"
ORIGIN      
        1 mtslketvss iemsvqhmsd tydkllevsn rhdkeiellr krvndieanq dgqqirklrq
       61 elndlsqysr rqnmeihgip vtagedllsk vnslavkldl ptlvakdieg lhrlpsqpdk
      121 vpvvlirfan rstktdwisk rkeceddirf fdnltassrn llwlakmkak emkhafvwsm
      181 egkiyarkqp garairiace edidkmv
//
LOCUS       XP_077501544             324 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-4-like [Amblyomma americanum].
ACCESSION   XP_077501544
VERSION     XP_077501544.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645418.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526901) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..324
                     /product="protein argonaute-4-like"
                     /calculated_mol_wt=36271
     Region          39..>312
                     /region_name="Piwi-like"
                     /note="PIWI domain. Domain found in proteins involved in
                     RNA silencing. RNA silencing refers to a group of related
                     gene-silencing mechanisms mediated by short RNA molecules,
                     including siRNAs, miRNAs, and heterochromatin-related
                     guide RNAs. The central component...; cl00628"
                     /db_xref="CDD:412485"
     Site            order(151,155,167..170,173,182,185,189,193)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     CDS             1..324
                     /gene="LOC144112620"
                     /coded_by="XM_077645418.1:1..975"
                     /db_xref="GeneID:144112620"
ORIGIN      
        1 mvaavvvhds klvkngthph yraneirqsv rdlvgssdqc lcefgikint eatqlmswvl
       61 dplslvfenn svgkprdgtw elrghhfykp amltrwtlln lselgmrieq pldmtttdan
      121 rkpirnillk eqrkttnlem viivlakntn yaeikqvaet eiglrtqcvm dnnvikkcna
      181 alitnlchki naklggtnns lltqekpdis qkpviiigad vthlapgdkl rpsiaacvgs
      241 ldsipskfha firvqmedsa atsrleiikd lkdmmkdmlk acycatkhrp eriiiyrdgv
      301 segqfmgvrk resylstahl qvli
//
LOCUS       XP_077501545             369 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112621 [Amblyomma americanum].
ACCESSION   XP_077501545
VERSION     XP_077501545.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645419.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526901) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..369
                     /product="uncharacterized protein LOC144112621"
                     /calculated_mol_wt=41520
     Region          <15..102
                     /region_name="Btz"
                     /note="CASC3/Barentsz eIF4AIII binding; smart01044"
                     /db_xref="CDD:214991"
     CDS             1..369
                     /gene="LOC144112621"
                     /coded_by="XM_077645419.1:1..1110"
                     /db_xref="GeneID:144112621"
ORIGIN      
        1 mkesagpsgl qvkvldcded krnpqyipkk gefyqhddrl vgdgdkseip eeknkdakgs
       61 rwqkklwred svwvhdmyye eeqgpktsee lvsiygydir tetmpprgrr rerygrgpnk
      121 yqrnwedeea ytprsgsgrg rgmrggrrpr ggghvgnppf qvkdipdlna krsvsesstt
      181 llqyqamvkv pnkpaafkrp dhgtrgnnsp qhgelehvak ssgtarrgwg krppqppvrl
      241 tntsskhhed qvkenmssdr haskpapqsp lktaessyrp psvgrsraar hmlmeqersr
      301 snaelhektv pkvelpdgga ayltknsaat veprggligg telagkprry sslrqrplae
      361 ylhqpkhql
//
LOCUS       XP_077501546             369 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112622 [Amblyomma americanum].
ACCESSION   XP_077501546
VERSION     XP_077501546.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645420.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526901) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..369
                     /product="uncharacterized protein LOC144112622"
                     /calculated_mol_wt=41520
     Region          <15..102
                     /region_name="Btz"
                     /note="CASC3/Barentsz eIF4AIII binding; smart01044"
                     /db_xref="CDD:214991"
     CDS             1..369
                     /gene="LOC144112622"
                     /coded_by="XM_077645420.1:1..1110"
                     /db_xref="GeneID:144112622"
ORIGIN      
        1 mkesagpsgl qvkvldcded krnpqyipkk gefyqhddrl vgdgdkseip eeknkdakgs
       61 rwqkklwred svwvhdmyye eeqgpktsee lvsiygydir tetmpprgrr rerygrgpnk
      121 yqrnwedeea ytprsgsgrg rgmrggrrpr ggghvgnppf qvkdipdlna krsvsesstt
      181 llqyqamvkv pnkpaafkrp dhgtrgnnsp qhgelehvak ssgtarrgwg krppqppvrl
      241 tntsskhhed qvkenmssdr haskpapqsp lktaessyrp psvgrsraar hmlmeqersr
      301 snaelhektv pkvelpdgga ayltknsaat veprggligg telagkprry sslrqrplae
      361 ylhqpkhql
//
LOCUS       XP_077501547             735 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112623 [Amblyomma americanum].
ACCESSION   XP_077501547
VERSION     XP_077501547.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645421.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526901) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..735
                     /product="uncharacterized protein LOC144112623"
                     /calculated_mol_wt=80984
     Region          89..196
                     /region_name="Btz"
                     /note="CASC3/Barentsz eIF4AIII binding; smart01044"
                     /db_xref="CDD:214991"
     CDS             1..735
                     /gene="LOC144112623"
                     /coded_by="XM_077645421.1:71..2278"
                     /db_xref="GeneID:144112623"
ORIGIN      
        1 mekegeasvs hgaagavqeg sheaasdslk spgnldsdqe epqeelelhp daskgdrnen
       61 sainddircl dkleddldek eeyclkgerq qgdgeetsdp sglqvkvldc dedkrnpqyi
      121 pkkgefyqhd drlasdgdkp eipeekneda rgsrwqkklw rddsvwvhdk yheeeqgpkt
      181 seelvstygy dirtepmppr grrrerygrg pnkyqrnwed eeaytprsgs grgrgmrggr
      241 rprgggqdgn ppfhvkdfpe lnvkrsvses sttlhqdqav vkvptkpacf nrpdrgtrdn
      301 hspqhreweh vakssgtarr gwgkcppqpp grltdtsskq hedevkenks sdrhaskpap
      361 qsplktaess hrppsvgrsr aarhmlmeqe rsrsnanlhe ktapkvelpd gaykssvfga
      421 sspffirlrl rschdviipr eihrhgqklf tivvslkknl yydrkkcavi ygtrngttas
      481 lycgaagltr nsaatvaprg gliggtevag kprrysslrh qplsevssyt kaavaapqlp
      541 naqappqrps qpqqailtaa hfqgpyppyp ddympppaqa pavrpppsqp apvmappaqm
      601 sspffpspdv ilsfppqhpl pytfpaqcaa papaappptq tqaqkppyyp gdtiyyntqs
      661 qqhkqrptpl rrpkaaipiv pppechgtvq egtceadgsa thvsksdddp ddsgavvtes
      721 alqeglagqq averl
//
LOCUS       XP_077501549             208 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112625 [Amblyomma americanum].
ACCESSION   XP_077501549
VERSION     XP_077501549.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645423.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526901) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 31% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..208
                     /product="uncharacterized protein LOC144112625"
                     /calculated_mol_wt=23079
     Region          <15..62
                     /region_name="RHOD"
                     /note="Rhodanese Homology Domain (RHOD); an alpha beta
                     fold domain found duplicated in the rhodanese protein. The
                     cysteine containing enzymatically active version of the
                     domain is also found in the Cdc25 class of protein
                     phosphatases and a variety of proteins...; cl00125"
                     /db_xref="CDD:444705"
     CDS             1..208
                     /gene="LOC144112625"
                     /coded_by="XM_077645423.1:1..627"
                     /db_xref="GeneID:144112625"
ORIGIN      
        1 mnitnakrlr eagypndail lgcnkiarkl kasaavpgsd rvkernkvav lllhlhqifl
       61 lhlacavvvr feisvqqsvt vcftffsqfh ltggiiiyeg qvekiltkkk ptlvckdtaq
      121 avwsnevlat ksfsgavapk qwalgekqkq pltpqkvefm iatmkhrgti kgvdiseavr
      181 nvpqmlteki qdvkkalrkf dvlnafkk
//
LOCUS       XP_077501550             703 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112626 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501550
VERSION     XP_077501550.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645424.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526901) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..703
                     /product="uncharacterized protein LOC144112626 isoform X1"
                     /calculated_mol_wt=77102
     Region          89..196
                     /region_name="Btz"
                     /note="CASC3/Barentsz eIF4AIII binding; smart01044"
                     /db_xref="CDD:214991"
     CDS             1..703
                     /gene="LOC144112626"
                     /coded_by="XM_077645424.1:71..2182"
                     /db_xref="GeneID:144112626"
ORIGIN      
        1 mekegeasvs hgaagavqeg sheaasdslk spgnldsdqe epqeelelhp daskgdrnen
       61 sainddircl dkleddldek eeyclkgerq qgdgeetsdp sglqvkvldc dedkrnpqyi
      121 pkkgefyqhd drlasdgdkp eipeekneda rgsrwqkklw rddsvwvhdk yheeeqgpkt
      181 seelvstygy dirtepmppr grrrerygrg pnkyqrnwed eeaytprsgs grgrgmrggr
      241 rprgggqdgn ppfhvkdfpe lnvkrsvses sttlhqdqav vkvptkpasf nrpdrgtrdn
      301 hspqhreweh vakssgtarr gwgkrppqpp grltdtsskq hedevkenks sdrhaskpap
      361 qsplktaess hrppsvgrsr aarhmlmeqe rsrsnaklhe ktapkvelpd gggaagltrn
      421 saatvaprgg liggtevagk prrysslrhq plseqpqist gqptevrfgc sgrllnirkq
      481 pvdvrrpsdg spkvssytka avaapqlpna qappqrpsqp qqailtaahf qgpyppypdd
      541 ympppaqapa vrpppsqpap vmappaqmss pffpspdvil sfppqhplpy tfpaqcaapa
      601 paappptqtq aqkppyypgd tiyyntqsqq hkqrptplrr pkaaipivpp pechgtvqeg
      661 tceadgsath vsksdddpdd sgavvtesal qeglagqqav erl
//
LOCUS       XP_077501551             700 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112626 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501551
VERSION     XP_077501551.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645425.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526901) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..700
                     /product="uncharacterized protein LOC144112626 isoform X2"
                     /calculated_mol_wt=76749
     Region          89..196
                     /region_name="Btz"
                     /note="CASC3/Barentsz eIF4AIII binding; smart01044"
                     /db_xref="CDD:214991"
     CDS             1..700
                     /gene="LOC144112626"
                     /coded_by="XM_077645425.1:71..2173"
                     /db_xref="GeneID:144112626"
ORIGIN      
        1 mekegeasvs hgaagavqeg sheaasdslk spgnldsdqe epqeelelhp daskgdrnen
       61 sainddircl dkleddldek eeyclkgerq qgdgeetsdp sglqvkvldc dedkrnpqyi
      121 pkkgefyqhd drlasdgdkp eipeekneda rgsrwqkklw rddsvwvhdk yheeeqgpkt
      181 seelvstygy dirtepmppr grrrerygrg pnkyqrnwed eeaytprsgs grgrgmrggr
      241 rprgggqdgn ppfhvkdfpe lnvkrsvses sttlhqdqav vkvptkpasf nrpdrgtrdn
      301 hspqhreweh vakssgtarr gwgkrppqpp grltdtsskq hedevkenks sdrhaskpap
      361 qsplktaess hrppsvgrsr aarhmlmeqe rsrsnaklhe ktapkvelpd gggaagltrn
      421 saatvaprgg liggtevagk prrysslrhq plseistgqp tevrfgcsgr llnirkqpvd
      481 vrrpsdgspk vssytkaava apqlpnaqap pqrpsqpqqa iltaahfqgp yppypddymp
      541 ppaqapavrp ppsqpapvma ppaqmsspff pspdvilsfp pqhplpytfp aqcaapapaa
      601 ppptqtqaqk ppyypgdtiy yntqsqqhkq rptplrrpka aipivpppec hgtvqegtce
      661 adgsathvsk sdddpddsga vvtesalqeg lagqqaverl
//
LOCUS       XP_077501552             664 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112626 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501552
VERSION     XP_077501552.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645426.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526901) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..664
                     /product="uncharacterized protein LOC144112626 isoform X3"
                     /calculated_mol_wt=72814
     Region          89..196
                     /region_name="Btz"
                     /note="CASC3/Barentsz eIF4AIII binding; smart01044"
                     /db_xref="CDD:214991"
     Region          <411..612
                     /region_name="PRK12323"
                     /note="DNA polymerase III subunit gamma/tau; cl46901"
                     /db_xref="CDD:481241"
     CDS             1..664
                     /gene="LOC144112626"
                     /coded_by="XM_077645426.1:71..2065"
                     /db_xref="GeneID:144112626"
ORIGIN      
        1 mekegeasvs hgaagavqeg sheaasdslk spgnldsdqe epqeelelhp daskgdrnen
       61 sainddircl dkleddldek eeyclkgerq qgdgeetsdp sglqvkvldc dedkrnpqyi
      121 pkkgefyqhd drlasdgdkp eipeekneda rgsrwqkklw rddsvwvhdk yheeeqgpkt
      181 seelvstygy dirtepmppr grrrerygrg pnkyqrnwed eeaytprsgs grgrgmrggr
      241 rprgggqdgn ppfhvkdfpe lnvkrsvses sttlhqdqav vkvptkpasf nrpdrgtrdn
      301 hspqhreweh vakssgtarr gwgkrppqpp grltdtsskq hedevkenks sdrhaskpap
      361 qsplktaess hrppsvgrsr aarhmlmeqe rsrsnaklhe ktapkvelpd gggaagltrn
      421 saatvaprgg liggtevagk prrysslrhq plsevssytk aavaapqlpn aqappqrpsq
      481 pqqailtaah fqgpyppypd dympppaqap avrpppsqpa pvmappaqms spffpspdvi
      541 lsfppqhplp ytfpaqcaap apaappptqt qaqkppyypg dtiyyntqsq qhkqrptplr
      601 rpkaaipivp ppechgtvqe gtceadgsat hvsksdddpd dsgavvtesa lqeglagqqa
      661 verl
//
LOCUS       XP_077501553             652 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112626 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077501553
VERSION     XP_077501553.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645427.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027526901) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..652
                     /product="uncharacterized protein LOC144112626 isoform X4"
                     /calculated_mol_wt=71293
     Region          89..196
                     /region_name="Btz"
                     /note="CASC3/Barentsz eIF4AIII binding; smart01044"
                     /db_xref="CDD:214991"
     CDS             1..652
                     /gene="LOC144112626"
                     /coded_by="XM_077645427.1:71..2029"
                     /db_xref="GeneID:144112626"
ORIGIN      
        1 mekegeasvs hgaagavqeg sheaasdslk spgnldsdqe epqeelelhp daskgdrnen
       61 sainddircl dkleddldek eeyclkgerq qgdgeetsdp sglqvkvldc dedkrnpqyi
      121 pkkgefyqhd drlasdgdkp eipeekneda rgsrwqkklw rddsvwvhdk yheeeqgpkt
      181 seelvstygy dirtepmppr grrrerygrg pnkyqrnwed eeaytprsgs grgrgmrggr
      241 rprgggqdgn ppfhvkdfpe lnvkrsvses sttlhqdqav vkvptkpasf nrpdrgtrdn
      301 hspqhreweh vakssgtarr gwgkrppqpp grltdtsskq hedevkenks sdrhaskpap
      361 qsplktaess hrppsvgrsr aarhmlmeqe rsrsnaklhe ktapkvelpd gggaagltrn
      421 saatvaprgg liggtevagk prrysslrhq plseqpqist gqptevrfgc sgrllnirkq
      481 pvdvrrpsdg spkvssytka avaapqlpna qappqrpsqp qqailtaahf qgpyppypdd
      541 ympppaqapa vrpppsqpap vmappaqmss pffpspdvil sfppqhplpy tfpaqcaapa
      601 paappptegt ceadgsathv sksdddpdds gavvtesalq eglagqqave rl
//
LOCUS       XP_077503811             354 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113915 [Amblyomma americanum].
ACCESSION   XP_077503811
VERSION     XP_077503811.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647685.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 29% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..354
                     /product="uncharacterized protein LOC144113915"
                     /calculated_mol_wt=38626
     Region          8..87
                     /region_name="GT1"
                     /note="myb-like, SANT family; cl23759"
                     /db_xref="CDD:474047"
     CDS             1..354
                     /gene="LOC144113915"
                     /coded_by="XM_077647685.1:1..1065"
                     /db_xref="GeneID:144113915"
ORIGIN      
        1 mektekgkin csrveralll dlvdkhkavl enkskrtdav svarkrkewe lietqfnssh
       61 nvsprtwlql kkcwenwknk wrkakaddnr eifktgggsa apsqltedlq rvgsvashmg
      121 vrlanpfdsd rhqadpgges qptpsvaall srtqdgsipg asgdaedplw swhyeegspq
      181 qdeqmeeepv amaasltsat tatatpsvlp aastaraagt srmssqrqsp avppapsgff
      241 vpatpaaaap spsvlpaasa esaagtsrta gmhrqslveq elaarlndis teraqkckeh
      301 rlrmrllrpd hrdklakraa vhqlemenlm lkndlkrskk illelqikav kgel
//
LOCUS       XP_077506928             100 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115895 [Amblyomma americanum].
ACCESSION   XP_077506928
VERSION     XP_077506928.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650802.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 11% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..100
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..100
                     /product="uncharacterized protein LOC144115895"
                     /calculated_mol_wt=10657
     CDS             1..100
                     /gene="LOC144115895"
                     /coded_by="XM_077650802.1:202..504"
                     /db_xref="GeneID:144115895"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrppfa altsvlccqr pasigpcvlp afgcvilpdp
       61 fmlktkscme vvqasklrvi catdleqagk ladgqwysls
//
LOCUS       XP_077507356             293 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116732 [Amblyomma americanum].
ACCESSION   XP_077507356
VERSION     XP_077507356.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651230.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 32% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..293
                     /product="uncharacterized protein LOC144116732"
                     /calculated_mol_wt=33303
     Region          55..143
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     CDS             1..293
                     /gene="LOC144116732"
                     /coded_by="XM_077651230.1:1..882"
                     /db_xref="GeneID:144116732"
ORIGIN      
        1 mapssttpvt lhnpkvpecf rgnafedvec rldqservak fnqwsvdqkl cnvyfalqdn
       61 astwfenhep rlstwddfrr hlvdtfaspd rrenalrlle vraqkpnesv amfeedmarl
      121 fcradpdmte qkklrhlmhg vkeqgfaglv rnppptisef kdataieral qewchqydrp
      181 fngvptaaaa ltatdritlr eliremvldk lrritatsvq ppevpaavrk eiryalspnt
      241 avitapyyep epqppsyavv lrrpppdhaq psspsrkqtg ffqgpqwapp grs
//
LOCUS       XP_077508359             265 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119342 [Amblyomma americanum].
ACCESSION   XP_077508359
VERSION     XP_077508359.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652233.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 47% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..265
                     /product="uncharacterized protein LOC144119342"
                     /calculated_mol_wt=29566
     CDS             1..265
                     /gene="LOC144119342"
                     /coded_by="XM_077652233.1:1..798"
                     /db_xref="GeneID:144119342"
ORIGIN      
        1 msmaasllas llpppkpldt sgnaclgspm wkkvlkglgq lkdfeynvkl krgaagvavp
       61 arrvplalqd kveaelqrmk eqgvikkplv ryqghiftee glrvdpqrvq dvlqmpahks
      121 yfvsaskdrl rdllaatdgd pallqlrgya etswpddkgd vpqvvrpyws yrqaihtqdg
      181 llfrnskspv qrlmgrqtrt lllvpaehlv petvpsiavh nrlqeirqcq rtyysrssrh
      241 lpplsqgqqi ttttrfigpg hllps
//
LOCUS       XP_077512728             189 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC144123568
            [Amblyomma americanum].
ACCESSION   XP_077512728
VERSION     XP_077512728.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656602.1
KEYWORDS    RefSeq; corrected model; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio            :: 6% of CDS bases
            frameshifts          :: corrected 1 indel
            internal stop codons :: corrected 1 genomic stop codon
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..189
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC144123568"
                     /calculated_mol_wt=20996
     CDS             1..189
                     /gene="LOC144123568"
                     /coded_by="XM_077656602.1:96..665"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 1 base in 1 codon;
                     substituted 1 base at 1 genomic stop codon"
                     /db_xref="GeneID:144123568"
ORIGIN      
        1 megpnsppae spardkylcp vckaefafxe srhvxnkhle aasssarekt facdlccnaf
       61 lhrrellqhh eqehgfmarr stvqfssiae fqawkettem eeqahfiakr gvtrlsprat
      121 krylechrtg vavqsgqgkr qpkavgsvkc nkhcfagmvv tqdgekvtve yqaehyghek
      181 sdtpalvrh
//
LOCUS       XP_077514806             517 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125153 [Amblyomma americanum].
ACCESSION   XP_077514806
VERSION     XP_077514806.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658680.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 20% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..517
                     /product="uncharacterized protein LOC144125153"
                     /calculated_mol_wt=57398
     CDS             1..517
                     /gene="LOC144125153"
                     /coded_by="XM_077658680.1:9..1562"
                     /db_xref="GeneID:144125153"
ORIGIN      
        1 millltcvgt llaaifvgly hkpwpfvllg iwhrqaedvp glrrltayhd ppqvaaplpp
       61 tldyvheatg rvdfrsfesv tqalrvvhsa keaipveslt iivplllslv ltgwwwykrr
      121 qsssttsads rtscrsnssl htlqesdeak hrrpggptlr syrlgrrrhs vpipsvqfpa
      181 lanfgpaest gtddsasvpv lrqrspltgf ltaseeqlfp lsvssdtdqp lnvggrlkrw
      241 lrdavwpdll gynpticggv ledfsaaeyh ggtlrteela prpqtssygg ctrddvnrad
      301 danrasteaf etpllddiyc rrsdasedgt detevvegnw adldesrpqr lewqpshhhq
      361 rderrrsgla dwelshvnns glsedtayla lpenkcpsee vlgacsfdqh qqnaccaaaa
      421 avapcfvspv eplraprhsq dlfeqllkac rqpeavafse ilqelgvetc vklhesehtd
      481 ifrvsgvrca pmvlkvfdcs yiarhvrclc neintsw
//
LOCUS       XP_077516015             227 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125789 [Amblyomma americanum].
ACCESSION   XP_077516015
VERSION     XP_077516015.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659889.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 27% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..227
                     /product="uncharacterized protein LOC144125789"
                     /calculated_mol_wt=24123
     Region          <162..200
                     /region_name="Kunitz-type"
                     /note="Kunitz/Bovine pancreatic trypsin inhibitor (BPTI)
                     domain; cl00101"
                     /db_xref="CDD:444694"
     CDS             1..227
                     /gene="LOC144125789"
                     /coded_by="XM_077659889.1:1..684"
                     /db_xref="GeneID:144125789"
ORIGIN      
        1 myartadmte dtlsvggnqv nklrkpdpri fdltlgpppt dapaphqgsl kmkdapsspe
       61 thtttqppte trspttessg ghttrttskt slgnmttekp tptrvpeqtt apdrspgane
      121 tasdkqgmkd ppvpttnssn dkdgtvcptv hksfcnvnga aqffydpdkg sclsatvqra
      181 hvcnrspnqf asiedclaac vkgrnaqkag drdirrpees apenssk
//
LOCUS       XP_077521348             148 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132437 [Amblyomma americanum].
ACCESSION   XP_077521348
VERSION     XP_077521348.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665222.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 39% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="uncharacterized protein LOC144132437"
                     /calculated_mol_wt=16037
     CDS             1..148
                     /gene="LOC144132437"
                     /coded_by="XM_077665222.1:1..447"
                     /db_xref="GeneID:144132437"
ORIGIN      
        1 mlgevvrlpd klklcddidp ytlrpgvdts advsgfpeis hgdivtylvf sanfvtlenm
       61 kaykaleshn yftsgwvkhl sakefqdgkv vllgevnhsq rlghkllqvw ilckkdgavi
      121 tahctcvaga geacsyvgac lqlrpask
//
LOCUS       XP_077522502             281 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133122 [Amblyomma americanum].
ACCESSION   XP_077522502
VERSION     XP_077522502.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666376.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 21% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..281
                     /product="uncharacterized protein LOC144133122"
                     /calculated_mol_wt=31795
     Region          50..236
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(108,147,161,163)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..281
                     /gene="LOC144133122"
                     /coded_by="XM_077666376.1:26..871"
                     /db_xref="GeneID:144133122"
ORIGIN      
        1 mhdarlrnly seemlahdld vlvkkandvm psiiiseetv kavealtrqq ssssnwflyr
       61 agrvtasvmk rvchtsidnp sisrlklicy pekqslktpa itwgvknepr afrayqtsea
      121 ekhdmfkcsk srlhlstryp fvgatpdglv ccafcgkgvv elkcpfslre vkdvtemdta
      181 gscletvngv vqlrrqhgyf yqvqtqmavc dvqwcdfvvw tpnwlhveri qkgrhfylpv
      241 npcgnaldal rtiishtvsp vgiilcqlmc gkflfvvlly r
//
LOCUS       XP_077524728             429 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136105 [Amblyomma americanum].
ACCESSION   XP_077524728
VERSION     XP_077524728.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668602.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 48% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..429
                     /product="uncharacterized protein LOC144136105"
                     /calculated_mol_wt=47743
     Region          177..251
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     CDS             1..429
                     /gene="LOC144136105"
                     /coded_by="XM_077668602.1:1..1290"
                     /db_xref="GeneID:144136105"
ORIGIN      
        1 macsgidfqs llwlsdmasq qqqvdaavtt tagvfaeada spcdlisfep sgdsrdtlaq
       61 tsgdarhfsp samppgmest aqpaqpcssh aapqpelpgq anpqeaalrn alrltesltg
      121 ivqnnlqtpa avarprlrvd lpsyggyhdp vsaneyldrm lnyqqatglt dgevlvrvvp
      181 vsltdqaarw frltghrsrt meefrarfre kflpadydrr lrrelelrtq hpdetlleyv
      241 ramdelyria dpsaqngekv ervtrqahpt faaylrgvqf rdlndlasad krieadilas
      301 rayrppppas qvleprcawn gdtfrirsrg aetvafsdgq lrngwelsdr aldpytyamr
      361 aahaarsqdv rndrpkkple tpsaelpyqp vdrterksak gtppfpcdpl fplrwhsapr
      421 rhaglptrs
//
LOCUS       XP_077485253             204 aa            linear   INV 25-NOV-2025
DEFINITION  THAP domain-containing protein 1-like [Amblyomma americanum].
ACCESSION   XP_077485253
VERSION     XP_077485253.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629127.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..204
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..204
                     /product="THAP domain-containing protein 1-like"
                     /calculated_mol_wt=23537
     Region          5..80
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..204
                     /gene="LOC144095013"
                     /coded_by="XM_077629127.1:1..615"
                     /db_xref="GeneID:144095013"
ORIGIN      
        1 mvgscvaydc tnsvkttpgv tfhkfpkdae vrtlwecavr regckakdgd rlcsvhftpe
       61 rfdrtgqttr lrpgtvpavf pafperlqap ikrkrpppks rdppcsedvk iwqhlmmmkr
      121 llylrlarne wfkdrlhase eetvhlkkki kilkqtkrrl qkrnetdlei irtikerkll
      181 sdegaevfga afssawvlak tfic
//
LOCUS       XP_077485587             316 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096152 [Amblyomma americanum].
ACCESSION   XP_077485587
VERSION     XP_077485587.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629461.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 25% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..316
                     /product="uncharacterized protein LOC144096152"
                     /calculated_mol_wt=34880
     Region          5..94
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..316
                     /gene="LOC144096152"
                     /coded_by="XM_077629461.1:1..951"
                     /db_xref="GeneID:144096152"
ORIGIN      
        1 mpedccvprc rgnydggerv rvfafpsdea rraewirair radftpgkrl mvcghhfkps
       61 dmvnrtsymd akigkvieva lklvrlrpda ipsilpdcla ylsapnasts reapnekgtr
      121 gesaasqdai slsieahqve edrnkihnfq allkcipcik vskfwtvvsq hdfilflnlt
      181 vdgaqaikks fkvtedlslk lyfhdvevtk ldgveiipkt vndvrcltgl ldavessdka
      241 pafrqpedkt ggilklcgvr rmhtkqpvkv aasgrapras sraardprsp aeeqssrslg
      301 glllrgvaeh atercy
//
LOCUS       XP_077486366             167 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097228, partial [Amblyomma
            americanum].
ACCESSION   XP_077486366
VERSION     XP_077486366.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630240.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         <1..167
                     /product="uncharacterized protein LOC144097228"
     CDS             1..167
                     /gene="LOC144097228"
                     /coded_by="XM_077630240.1:<1..504"
                     /db_xref="GeneID:144097228"
ORIGIN      
        1 ssssssssss ssssssssss ssssssssss ssssssssss ssssssssss ssssssssss
       61 ssssssssss ssssssssss ssssssssss ssssssssss ssssssssss ssssssssss
      121 ssssssssss ssssssssss ssssssssss ssssssrnsg fppptpg
//
LOCUS       XP_077487209             283 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098207 [Amblyomma americanum].
ACCESSION   XP_077487209
VERSION     XP_077487209.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631083.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..283
                     /product="uncharacterized protein LOC144098207"
                     /calculated_mol_wt=31127
     Region          27..141
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     Site            order(31..34,62..63,66,85,122,140)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:259998"
     CDS             1..283
                     /gene="LOC144098207"
                     /coded_by="XM_077631083.1:1..852"
                     /db_xref="GeneID:144098207"
ORIGIN      
        1 mskedhsgrr laraeslayh ygdkhgvfyv dasgphrggw ytaavvhkka tvnrltfraq
       61 nithaeevai alaaadrdsr viitdssgac rnidqgyihf layrilqnsd ypgapaprti
      121 vwapahlgle gnemadasar altlrvttps nptaanpdpk pvftfkeitq lyqsehsivp
      181 kpckdltkae ehillrlftk tllcpavlkf fdpactgkcp hceekssdiy hmvwacqstp
      241 nldlkpnptp edweeallnc sdltsqkalv vrvraalevn gll
//
LOCUS       XP_077489258             414 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099628 [Amblyomma americanum].
ACCESSION   XP_077489258
VERSION     XP_077489258.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633132.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..414
                     /product="uncharacterized protein LOC144099628"
                     /calculated_mol_wt=43783
     Region          <201..>246
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     Region          240..>397
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..414
                     /gene="LOC144099628"
                     /coded_by="XM_077633132.1:1..1245"
                     /db_xref="GeneID:144099628"
ORIGIN      
        1 mgshgaaiaa vagrgnrfas dadkdyeivl ptlptgrvvl ntvflhgdvr arpyrvedfr
       61 dalanagalp dvvalgayqi nhvwavtfst adaakklaat kelevkgrrc ivfdpedhqv
      121 klrlhwmlhg vadedirtaf aafgnvtevt rerwrvagmk ekgsttrtvl lklkpgvkvd
      181 dlphqvrvag elalvvvpgr pmqclrcggt ghvrrdckvp rcskcrrygh seadcvrtya
      241 satghvaasv saelmdvvea eeaasgaddt gkaegvpvqp avassntdta vaqesgspat
      301 dpsstpvetp kdgasssvhv ereaaseshd ekddsrinvg vttpakrpha etkadgekaa
      361 epsvgeppak tpqarraglr prpkvpaekr ggdkappdqe hvlapddtgg dgiv
//
LOCUS       XP_077496357             422 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106952 [Amblyomma americanum].
ACCESSION   XP_077496357
VERSION     XP_077496357.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640231.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..422
                     /product="uncharacterized protein LOC144106952"
                     /calculated_mol_wt=45742
     Region          <200..>285
                     /region_name="COG4372"
                     /note="Uncharacterized protein, contains DUF3084 domain
                     [Function unknown]"
                     /db_xref="CDD:443500"
     CDS             1..422
                     /gene="LOC144106952"
                     /coded_by="XM_077640231.1:1..1269"
                     /db_xref="GeneID:144106952"
ORIGIN      
        1 mvgteddqlw epgngvsssd sgenadevns sdgirllaca slllpslagl stgassvcal
       61 pytgddpgsc hlppdecssl ldvppeelht peitvpwvat stsatmptci kelhpltplr
      121 glgdcttalp pnpltfamqv stsyalfakk ssnyilmqfp sphccvaiav ecahvihsll
      181 mlsgdvetnp gpggnaavla elqklnagqt lliteiqglk iqlnttdqti asldkrmadl
      241 eahyqtllhl rndieimqst ttnqakkiee letrlddaen qsrrnnlify gipdpasaet
      301 waeseklvid vcrnnlditl epndierahr lgnhsadrtr pvivkflshk tkdallsngr
      361 klkdtnysig edfsrtvlha ckqllvfaka nsnkcslrfk tlhigskryv fdassqtvke
      421 ia
//
LOCUS       XP_077497371             146 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107743 [Amblyomma americanum].
ACCESSION   XP_077497371
VERSION     XP_077497371.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641245.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..146
                     /product="uncharacterized protein LOC144107743"
                     /calculated_mol_wt=15848
     CDS             1..146
                     /gene="LOC144107743"
                     /coded_by="XM_077641245.1:283..723"
                     /db_xref="GeneID:144107743"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrppfg altsvlccqr lasigpcvlp afgcvippdl
       61 fmlkskscme vvqatklrmm fardleqagk lvdgqwysls kasffsktkt iflgswamhl
      121 gtcgqdnagq hasasskkkh etvnky
//
LOCUS       XP_077498899             225 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109701 [Amblyomma americanum].
ACCESSION   XP_077498899
VERSION     XP_077498899.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642773.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..225
                     /product="uncharacterized protein LOC144109701"
                     /calculated_mol_wt=26033
     CDS             1..225
                     /gene="LOC144109701"
                     /coded_by="XM_077642773.1:1469..2146"
                     /db_xref="GeneID:144109701"
ORIGIN      
        1 mnpprsgtee eyeekekllq eisdlarefr ykcksrkaqa aanktfatgv rdnacaaaar
       61 vsphhsdsyp tpsgtandtp etasyvlqqm csdrdvvddy fttdaenfqs aqqtasanvt
      121 tdvqeeteeh epqptaemkr rkrkpktagd enheflekrw sheramkekd mqlemeraal
      181 ekvrleikqr ryekrqqmks ikmenefklr elelearane vqarr
//
LOCUS       XP_077499929             356 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144110491 [Amblyomma americanum].
ACCESSION   XP_077499929
VERSION     XP_077499929.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643803.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 39% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..356
                     /product="uncharacterized protein LOC144110491"
                     /calculated_mol_wt=39245
     CDS             1..356
                     /gene="LOC144110491"
                     /coded_by="XM_077643803.1:1..1071"
                     /db_xref="GeneID:144110491"
ORIGIN      
        1 maasslpfec eldsdncven vscfrvalnt dgdayewlts ygvatrttwi vdwevakpkr
       61 iafhkkwkcq hsnrnkitgq tatncpafvd ikiknitrdt rkrdpflkrv tplraivnvr
      121 ddhnhaldca dvlrllrtta dtravfhsyf hdgltpaqai amhqqkleae ddaaeklasg
      181 avnpsagtvy ywfrlwrdhh ygsavdpvsk lsekapcyld kgadvrttrs edgtcwavlv
      241 vtqvmkrtqg lsaakeiifv dstascdesq ssltvvltat avgamplavl lhssqssesy
      301 kaafgllkls yptcfggthi myastandln taiaemkaks hkgyvarvlg flerka
//
LOCUS       XP_077500978             720 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111265 [Amblyomma americanum].
ACCESSION   XP_077500978
VERSION     XP_077500978.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644852.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..720
                     /product="uncharacterized protein LOC144111265"
                     /calculated_mol_wt=80848
     CDS             1..720
                     /gene="LOC144111265"
                     /coded_by="XM_077644852.1:250..2412"
                     /db_xref="GeneID:144111265"
ORIGIN      
        1 mgesalkshm ksakhsacmk raaagsvrey fsasasarpv dtpsqssdvn iackvnelvt
       61 daeilwtlkl vtthcsyrss seagelfkrm fpdseiakgf scgerkcayi tchgltpffl
      121 sslqrdierc enyvvlfdes akewlqekqm dvhirfwdss rkvvtkyyts vfmghataed
      181 ikdkllsale plplakilqi smdgpnvnlk ffkmlqshlq qnfqvhcldl gtcslhtvyn
      241 aykagiiask wgldtllssl salfedapar rhdfsaltgk avfplkyvah rwvenvavie
      301 ralelwsdvk kfvesarkkq vslpkcvsfq nivefiqdtl vvaklnfals vaatlkpfll
      361 efqtdklmlf llardletiv rklmtkfikc stlssatgit gmrqidiedp knhtplekvd
      421 vghtieqilk kcnasakdif qfkmeckqfl vavtkkvlek splrftvvrg lssldtrkmc
      481 crpneclagl kkvlealiaa grltdqrrds vlaeytellq ekhqlrlfek atsldtffse
      541 llqfnasyae lwkvvksllv lshgqatver gfsinrqisv enlkglsyis qrivcdavek
      601 aggifnivii kdlrkavsaa thhysaylea qkrleqecvk qskkrcaeaq ldslnkkrki
      661 leaavsdlta saddhaekae aandltyiik snymrkaaka kaeqllnlnk eieaklqevs
//
LOCUS       XP_077501712             191 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112737 [Amblyomma americanum].
ACCESSION   XP_077501712
VERSION     XP_077501712.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645586.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..191
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..191
                     /product="uncharacterized protein LOC144112737"
                     /calculated_mol_wt=20779
     Region          103..173
                     /region_name="BEN"
                     /note="The BEN domain is found in diverse animal proteins;
                     smart01025"
                     /db_xref="CDD:214981"
     CDS             1..191
                     /gene="LOC144112737"
                     /coded_by="XM_077645586.1:1..576"
                     /db_xref="GeneID:144112737"
ORIGIN      
        1 mrlmadtnai kelwnshvpm nleygeppem aklldcgeht akmqrrraki sqpgtassss
       61 agdssaespq ggddleyigg clvekfvlkm ldvglprkta styarellrq vfdpaelvnk
      121 sitgrqsnah krkdakpqld pfrvnaviky tckkfglire apirqslssi lnkhkaeved
      181 lnggaagdgg k
//
LOCUS       XP_077501793             189 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112825 [Amblyomma americanum].
ACCESSION   XP_077501793
VERSION     XP_077501793.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645667.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 27% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..189
                     /product="uncharacterized protein LOC144112825"
                     /calculated_mol_wt=21186
     Region          2..189
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(43,79,92,100)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..189
                     /gene="LOC144112825"
                     /coded_by="XM_077645667.1:1..570"
                     /db_xref="GeneID:144112825"
ORIGIN      
        1 mqscsttwnf erskrltash fgdvvsrqkp adekffkrll riedaavqry igkrtvpvrs
       61 yccglcvnpg vpvlgatpdr vveekgdfdl levrtlsaak ergdelenav ntasylknrv
      121 lkgtgmfglp calkpmhkyf yqvqgqlalt glswcdfvvd ngtdstfqri afenslwisk
      181 mlpcllecy
//
LOCUS       XP_077501881             609 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112903 [Amblyomma americanum].
ACCESSION   XP_077501881
VERSION     XP_077501881.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645755.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 24% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..609
                     /product="uncharacterized protein LOC144112903"
                     /calculated_mol_wt=68886
     Region          <178..262
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     Region          283..377
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(299,301,303,331..333,370)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133137"
     Site            299..301
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133137"
     Site            299
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133137"
     Site            order(331..334,338..341)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133137"
     Region          <468..492
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Region          <557..592
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     CDS             1..609
                     /gene="LOC144112903"
                     /coded_by="XM_077645755.1:163..1992"
                     /db_xref="GeneID:144112903"
ORIGIN      
        1 mavgrigeyr lgtnaswdey verlemfcea nqitkeeqkr avllsscgee vyglivtlvk
       61 perptaatye eiktavrkhl hpkpselyar flfyrrnqaa eesvadyvta lrklaedcgf
      121 gsaqlpldvm mrdrfvcglr negvqqrlla ehsltfkvay dlattaeata kqqrdirkhg
      181 ryetdcqemv qatrakqdtp aegsscyrcn gkhaphlcrf rkatcfnckr vghiakacrs
      241 kddsrtarkt ayeqkkkgdk skgvyemsal fslcevneqe qkfmvqvtie gqevpmevds
      301 gascsivsed tfrvvernhg kiplqdsgtt ivtwskealp vlgqasvlve fkgrkaklpl
      361 lvvqkqgnsl igrnwfnplg iglrgiqqvn vedvpsrfpe vfrrdlpgfn gppvhielkd
      421 daqpvflksr pvplalkddv anevdrlvqq gvwepvsysn watplvvkaq lrvnkdkcef
      481 nqtsieflgh riddsgvhps rsktdaiqqa papaskkelq aflgllnfys sflkgrteaa
      541 eplyrlldrd hewkwtgmsa mkasarglmw wpkmdheiee fvrhchpcqn nrqsdakapv
      601 hfwikpdqp
//
LOCUS       XP_077501985             481 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112976 [Amblyomma americanum].
ACCESSION   XP_077501985
VERSION     XP_077501985.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645859.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..481
                     /product="uncharacterized protein LOC144112976"
                     /calculated_mol_wt=53677
     Region          4..94
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          262..462
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..481
                     /gene="LOC144112976"
                     /coded_by="XM_077645859.1:403..1848"
                     /db_xref="GeneID:144112976"
ORIGIN      
        1 mgrccvpgcr gnydtgpkvr vfafpkdasr reswiraipr kdftpsvhsk vcelhfqend
       61 lirklshfda asgktltvei ervhlvsdav psifpncpay lstnhtrres paskrarmed
      121 ealgkaikss metkkdeeqr iaiasfqdlk hklsgignsd fwtisvnsrc vmflrieenp
      181 sphvrfsvtv fadlslsvsa gmiklnslpc ggaipeavrd tstlsvlleq lekhmtetse
      241 ettasktttv lqhigsllne lteesdatke lsalirllhe qitltlagpi rrysadtlvf
      301 ssilhsisph aynfarsasm ltlphpstlr rvcskhggdp mkeqneahfl syirervkfm
      361 eshektvtlm ideihikpyf dykggsivgs sansqeaatt ahvfmiqsll sankdvihil
      421 pvakmnseil hdyckriile meswglkvia vitdnkslnr kmmslfsenr edavnkskkp
      481 p
//
LOCUS       XP_077502373             255 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113241 [Amblyomma americanum].
ACCESSION   XP_077502373
VERSION     XP_077502373.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646247.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..255
                     /product="uncharacterized protein LOC144113241"
                     /calculated_mol_wt=29342
     CDS             1..255
                     /gene="LOC144113241"
                     /coded_by="XM_077646247.1:171..938"
                     /db_xref="GeneID:144113241"
ORIGIN      
        1 mdllkvpepl rlsgnlsenw lrfkqkfely lqatavkkap ksdaskaall lsfagdeald
       61 ifnnfqyspg eskksygtvv qkfnayfsev sneiheryvf rtksqmekep fekfarylkr
      121 qatrcnfgdq hdslirdqiv fgtnnsklre rmlaekdltl lkaeelaalr sqvwnksedc
      181 vdsissrtvs tkkqqqrdkk lcrnnqeycc rkcnrkhrpk qcpafgiich schkfnhfas
      241 csrksttvse vgkad
//
LOCUS       XP_077502477             465 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113316 [Amblyomma americanum].
ACCESSION   XP_077502477
VERSION     XP_077502477.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646351.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 18% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..465
                     /product="uncharacterized protein LOC144113316"
                     /calculated_mol_wt=50951
     Region          <300..>432
                     /region_name="PRK03918"
                     /note="DNA double-strand break repair ATPase Rad50"
                     /db_xref="CDD:235175"
     CDS             1..465
                     /gene="LOC144113316"
                     /coded_by="XM_077646351.1:1..1398"
                     /db_xref="GeneID:144113316"
ORIGIN      
        1 mvycvptvtr rrsaprrtts tydlavssln fflasgclav vgfmfgfgtf atgllasacl
       61 aamaiplafe asprrtrfrw scraalaaas rclsnfagqp whgsslsgnm lrnpfcfvlq
      121 vvalllrtel lscsfraalr hneslgaypv sstcvgdicl vddptapvii atpmptavts
      181 dhmtrfpaik getalslisg hgsslsgnml rnpfcfvlqv srylptkcyr sdnrflvllp
      241 splplmnvfd yvisiprdll vcgdveanpg ptekemlsel ldgqgkltaa vealhysqtd
      301 iernisaltd rieglekqlv glhalnnrvn viedtvakvd kqlsaliskv ddlenrsrrn
      361 nlviyglaee deetvadler klitevfqdk leltvtgter chrlgrkigd kkrpvivkfi
      421 dyrekvsalk sayklkgsat sisedfyqna gntkapveec htgkr
//
LOCUS       XP_077502569             184 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113372 [Amblyomma americanum].
ACCESSION   XP_077502569
VERSION     XP_077502569.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646443.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 21% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..184
                     /product="uncharacterized protein LOC144113372"
                     /calculated_mol_wt=20108
     CDS             1..184
                     /gene="LOC144113372"
                     /coded_by="XM_077646443.1:1..555"
                     /db_xref="GeneID:144113372"
ORIGIN      
        1 mdmnqqekae ftnrgraygv dsevkdllek ssdpylallt yrdtpgvsgv spaqlsigrk
       61 lqtrlprteh qplpshemfr aqdsatnvql gknytcrhsa gppsplkpgd dvwvkdigcs
      121 gqvlspaqrp rsyvvetpgs ilqrnrrhlv rfstgqnile qvaaspskpe rsqqssscsl
      181 pvgp
//
LOCUS       XP_077503354             494 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113849 [Amblyomma americanum].
ACCESSION   XP_077503354
VERSION     XP_077503354.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647228.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..494
                     /product="uncharacterized protein LOC144113849"
                     /calculated_mol_wt=54541
     Region          32..>167
                     /region_name="SWIM"
                     /note="Uncharacterized protein, contains SWIM-type Zn
                     finger domain [Function unknown]; COG4715"
                     /db_xref="CDD:443750"
     Region          288..481
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(351,390,408,410)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..494
                     /gene="LOC144113849"
                     /coded_by="XM_077647228.1:79..1563"
                     /db_xref="GeneID:144113849"
ORIGIN      
        1 mmacpgqhlv pggflgltdg wvrsprqaav pteeqilqyl qssgmrcdrq lskgrrfrde
       61 gyirnimfne ispvsevgvf rciclpsmkg gyyivhavae kttgditeah chcpaglsgt
      121 cqhvvgllla vvgaaaepep tctdlpcawi vpssakkvev akplsditfk ttdpngrqgf
      181 krkrsydpcp ngmpsdagcf rrklqnafpr alwlrynkea teqpsvslea neneqptaev
      241 tsahqgvpal mdlikehcql tgpeflayik qaytgedisq ieektklqsl ssqwmvyrrg
      301 mvtasrarac ltrtrsldke prphnlrgiv nlvlqsatfk ssamneglrk eaeakafyis
      361 nleksghtas iadvgltifr evpivgaspd givtsncecc mgtvrvlevk cplqlknsfk
      421 dlnekkpkli yqtqmnvtmg ilnipecdfi vycnkdevat vtikydaagf neflqaaqkl
      481 fieylypalq cect
//
LOCUS       XP_077503693             311 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger protein klumpfuss [Amblyomma americanum].
ACCESSION   XP_077503693
VERSION     XP_077503693.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647567.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 20% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..311
                     /product="zinc finger protein klumpfuss"
                     /calculated_mol_wt=32888
     Region          176..197
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          205..>262
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          209..229
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(214,216,218,220..221,224..225,228,242,244,248..249,
                     252..253,256,270,272,274,276..277,280..281,284)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          237..257
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          265..285
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..311
                     /gene="klu"
                     /coded_by="XM_077647567.1:1..936"
                     /db_xref="GeneID:144114065"
ORIGIN      
        1 mvigpaedgq apfakeagtd nsrtylhglm lnvlrnrrpr pparaagavr sgggaaapig
       61 apggrprrrs appdaeraat ttgvsadgrr raawaaaaag rsmaepdsss mtsgaearkr
      121 rrlsavldkl rsrsdddafs ppsdsssslc gcrqcaaeld apldlslaaa aaahvcsacg
      181 qafaqrdrla khvasrhraa asdgrvyacd vcgrsfarsd mltrhmrlht glkpytcrvc
      241 gqvfsrsdhl sthqrthtge kpykcplcpy aacrrdmitr hmrtharyep pdssgssldg
      301 daalskepgg r
//
LOCUS       XP_077503923             303 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114184 [Amblyomma americanum].
ACCESSION   XP_077503923
VERSION     XP_077503923.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647797.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..303
                     /product="uncharacterized protein LOC144114184"
                     /calculated_mol_wt=34353
     Region          8..97
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..303
                     /gene="LOC144114184"
                     /coded_by="XM_077647797.1:11..922"
                     /db_xref="GeneID:144114184"
ORIGIN      
        1 msrrqshcfv pgcttgyksc keklslfgvp kdeelflkwq rnipradkpl ernaavcelh
       61 fdpqfvlrhf ehniqgktvr mersrpvlqp davptkfpnv pkylsmpvpk krnpkerldp
      121 qvvpskrrrt vpqasdvsae apasaepdll rtsdarnvtl psdkwgkhvf ceepltvsys
      181 vclpgpnmgl lhaqklvlfr segtavkhqv fvrgveltai dekcdpasvl qevdsmqvca
      241 gaglltefch vscskmikws egfyhirckg avsenemrcv ackylrrlli nqrcrekkrn
      301 vre
//
LOCUS       XP_077504372             366 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger Y-chromosomal protein-like [Amblyomma americanum].
ACCESSION   XP_077504372
VERSION     XP_077504372.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648246.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..366
                     /product="zinc finger Y-chromosomal protein-like"
                     /calculated_mol_wt=42678
     Region          20..122
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(73..78,89..91,112..115)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Region          <143..327
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          149..175
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          180..200
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(185,187,189,191..192,195..196,199,213,215,219..220,
                     223..224,227,241,243,245,247..248,251..252,256)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          208..228
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          236..257
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          265..303
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          311..332
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          340..356
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..366
                     /gene="LOC144114459"
                     /coded_by="XM_077648246.1:1..1101"
                     /db_xref="GeneID:144114459"
ORIGIN      
        1 mskfefltsg kstvkgvpeg vftlkalpkg rhfgpyhgvk vdgienggrt wpvcrcaeff
       61 vvdgrqqery hwmnkvnysp skrrqnlval lfngdiyyrt irnigpgeel lvgysvsfae
      121 sllgnprgrg alkeedgdiv plnslflace krgdlfstqy nlemhtrrkh wnrthgihrc
      181 tyctytssrk fniirherth vgrreslcpl chkrfshlkn lkvhmkihse ekpyecgqcg
      241 krfrqrvhar rheqvihsrr yqlhcprcga gaedvtklkr htcnplkksf yqpknlyvhm
      301 kihsgekpye cvqcgkrfrq rlhgrrheqv ihsrryplhc prcgagaedv tklrrhtckp
      361 lkvkkn
//
LOCUS       XP_077504486             108 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114523 [Amblyomma americanum].
ACCESSION   XP_077504486
VERSION     XP_077504486.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648360.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..108
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..108
                     /product="uncharacterized protein LOC144114523"
                     /calculated_mol_wt=11698
     CDS             1..108
                     /gene="LOC144114523"
                     /coded_by="XM_077648360.1:392..718"
                     /db_xref="GeneID:144114523"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrppfa altsvlccqr pasigpcvlp afgcvippdp
       61 fmvktkshme vvqatklrvi fardleqagk lvdgqwysls kasffsmp
//
LOCUS       XP_077504601             305 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114593 [Amblyomma americanum].
ACCESSION   XP_077504601
VERSION     XP_077504601.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648475.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 21% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..305
                     /product="uncharacterized protein LOC144114593"
                     /calculated_mol_wt=33842
     CDS             1..305
                     /gene="LOC144114593"
                     /coded_by="XM_077648475.1:183..1100"
                     /db_xref="GeneID:144114593"
ORIGIN      
        1 mekfafvksl nmvkerglkv asvttdrhgq vtkymrtqep tirhyydcwh iskgikkkla
       61 ahakragcsv lkiwiqpasn hlywcaalsd gsaelltdmc ksmerhaani htghpglyth
      121 cahdelgere wlvpgttahn kfvevvsspr ilkdirqlap cthtfsleaf hsvligfapk
      181 svcfspegmr artqlailhf nenaskgqat taeglsrwki khpkarkgta vacpvkaeps
      241 ydyvglllke tvhcceqwps fkaafaenls tapppmsrsf prpsknelva arrsrfassm
      301 gsttv
//
LOCUS       XP_077504711             129 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114666 [Amblyomma americanum].
ACCESSION   XP_077504711
VERSION     XP_077504711.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648585.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 38% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..129
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..129
                     /product="uncharacterized protein LOC144114666"
                     /calculated_mol_wt=14427
     CDS             1..129
                     /gene="LOC144114666"
                     /coded_by="XM_077648585.1:125..514"
                     /db_xref="GeneID:144114666"
ORIGIN      
        1 mingkaagnv lmsaamlfag tsptsvlrmf nyvnvqvftp rtfynyqrgy llpaidkiwq
       61 qqqeelfrql egctvdlagd grcdspgfca kfltyslhva qlnkilhfeq vqvgevghsf
      121 likwlcdvt
//
LOCUS       XP_077504923             298 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114795 [Amblyomma americanum].
ACCESSION   XP_077504923
VERSION     XP_077504923.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648797.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 22% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..298
                     /product="uncharacterized protein LOC144114795"
                     /calculated_mol_wt=33899
     CDS             1..298
                     /gene="LOC144114795"
                     /coded_by="XM_077648797.1:602..1498"
                     /db_xref="GeneID:144114795"
ORIGIN      
        1 mnpwedpriy cetlatirlv dvlahhhsiv vveltsfvar nhfllnllqe kygirkltis
       61 dvpyceanvl ealqklanps eqnyaharke yfcfsvrlsl slfaqeqgsv tlttldvani
      121 ymsaggagql idtllqnnti sdltvgacvl tyggvyllqg fvgylekers plkkltvrtp
      181 earrralesl vkgitptttl eelvididiy dvedkahfte iiarnrtlrt lsvlwrkktg
      241 qrrfhqrtge heykdealle rgdhqisssp rrkpveakee vfksfsfwas ftkvrrla
//
LOCUS       XP_077505030             152 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114866 [Amblyomma americanum].
ACCESSION   XP_077505030
VERSION     XP_077505030.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648904.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 28% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..152
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..152
                     /product="uncharacterized protein LOC144114866"
                     /calculated_mol_wt=16428
     CDS             1..152
                     /gene="LOC144114866"
                     /coded_by="XM_077648904.1:1..459"
                     /db_xref="GeneID:144114866"
ORIGIN      
        1 mvrlgvkmsl hfdgavtpiv lasaiafvgl pllvflgvmm rhrqsalpll tdlkvpaliv
       61 ieslifagte vvldlcleal hkgrhsasfa vlvltrllyt vwlavlfras dpllasglav
      121 rvaklarial ldltcmqrle etlelclgdm tr
//
LOCUS       XP_077505244             221 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114993 [Amblyomma americanum].
ACCESSION   XP_077505244
VERSION     XP_077505244.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649118.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 40% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..221
                     /product="uncharacterized protein LOC144114993"
                     /calculated_mol_wt=22577
     CDS             1..221
                     /gene="LOC144114993"
                     /coded_by="XM_077649118.1:121..786"
                     /db_xref="GeneID:144114993"
ORIGIN      
        1 mdcsqslset sasvrragep aavsgepvsk gyvqsrssqs lllfqedadg asptgstlnq
       61 acshasgfim vqsdhtyagp steasassle pgtagvaatl sasadlspga pfsssplsye
      121 gsftspiner rplqkvraql rqllvsksge igasaagava gpphgaaisp rslaarvprv
      181 ttnfplalar vfittdkflv risatllvng rvdeecekks v
//
LOCUS       XP_077505547             331 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115170 [Amblyomma americanum].
ACCESSION   XP_077505547
VERSION     XP_077505547.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649421.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 34% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..331
                     /product="uncharacterized protein LOC144115170"
                     /calculated_mol_wt=37340
     CDS             1..331
                     /gene="LOC144115170"
                     /coded_by="XM_077649421.1:1..996"
                     /db_xref="GeneID:144115170"
ORIGIN      
        1 mdlkalkkpe slslakelgr qiaeskrkpe ileaieaiga dnveleewre siaekeeerk
       61 rleeeekeel astddacsts dqpainemla ellagqrkma ediadikrfq qrvnsrfeal
      121 esrvfaless sptsngdasd gavqrqlnsl yevvsklasr ndelenrsrr nnlifhglde
      181 edeetnkalf iavtkvfidn lqldcpkier yhrlgrkqeg gprpvivkll dfrektqalk
      241 nahrlkhsgi slpedfsahv rlirkkiwda mqshrdsgsk vhlrydhvfi dsvlynwddv
      301 ekslvkdsks tppdtaaaan ataakppads d
//
LOCUS       XP_077505661             258 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115220 [Amblyomma americanum].
ACCESSION   XP_077505661
VERSION     XP_077505661.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649535.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 18% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..258
                     /product="uncharacterized protein LOC144115220"
                     /calculated_mol_wt=29246
     Region          10..102
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     CDS             1..258
                     /gene="LOC144115220"
                     /coded_by="XM_077649535.1:1..777"
                     /db_xref="GeneID:144115220"
ORIGIN      
        1 mprelfnell iaevakrpll wdmkdnnfrn rawkealwee vgdalrknya tvtteevmnr
       61 wknlkdtfrk kikdeadskr sgaaagikss wpymqqmefl rdtvetrrsf cnlaptdlsv
      121 gsaelhmasp vsnekepetp ldeleaifgg pgytegseag llpaptqsts avplrgikrq
      181 kkrqaiqeae relhsvrnai tslrpmdass hfllslkpqm datpphmlmh lkmellhvce
      241 qysqgqyptd mavrvphe
//
LOCUS       XP_077505861            1131 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: WD repeat-containing protein 11-like
            [Amblyomma americanum].
ACCESSION   XP_077505861
VERSION     XP_077505861.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649735.1
KEYWORDS    RefSeq; corrected model; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio   :: 1% of CDS bases
            frameshifts :: corrected 2 indels
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1131
                     /product="LOW QUALITY PROTEIN: WD repeat-containing
                     protein 11-like"
                     /calculated_mol_wt=124643
     Region          35..83
                     /region_name="WD40"
                     /note="WD40 repeats; smart00320"
                     /db_xref="CDD:197651"
     Region          49..95
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          352..752
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          417..453
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          458..504
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          512..547
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          642..676
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          682..725
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          726..761
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..1131
                     /gene="LOC144115348"
                     /coded_by="XM_077649735.1:1..3396"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 2 bases in 2 codons"
                     /db_xref="GeneID:144115348"
ORIGIN      
        1 mvhcklpkac vvtgfnsllg ecchkxvvvi dtkykkvfqt lsxhqhnvtk vcwsrnlhyh
       61 dlvnpyhlkl asadssgtll vwdvsqatvr stlveldrtl lelswllggd hhlllalysl
      121 gtlvlwhadt gaqlwrkcya eplisfsldp fcestvcfla vdhlllvedl sgqqapasgg
      181 hkwssiascp sdaaaeaksk prsrllsgvk slirtgdart sieealplne clqlcyhqav
      241 rhqlfllypk kifvmdtqvq qvlgtipler ngsaflrcrp cwqcdflfcl hesgsvsars
      301 rhegsgslag eldqacgivg ysthctseal rlskhskvag mavclssqck valllgdgri
      361 vflklsvtek rlapekplrl atligdsrag slrlitagll ealapgphls rmcppvtfgn
      421 wstyrpllav gsttgsvqvf dlssgtlere lnlhatpvrg iewvsltsfl sfayanlvtv
      481 gskvrnevvl tsiqsgrvqh vradaneesp itcikvsylk qyfvvlfkdq pfelwdlrnl
      541 vllrtmpdsf pcvtalewsp lvsskaqlra rhlagskdve pifansateq lerqlgqqta
      601 spllreqlvf tdteaqlyyf svegnvvrdc trnppeagma sitsiawksd hivlgdaegt
      661 ltvwdlkgkv lralptqrgy vkklkfapgk gnmkilalfa dginawdaqd vqlfiqvrcp
      721 qdlpgitdvd waksdklvva tsdgcirvmd memkscsspv rdhlqsdrvp fvpqllppka
      781 alllksllqh pdltpgiapk qlpeeaslye rlqlaslqcr vparadvvqr slavagwfgd
      841 eeglhfwsva qyyltknqqn veeggtapla lyydmlcpda aykalqlerl llherqrvlh
      901 shtqrcasrl lllgqtdqav qllletepgs ehfysdslra clvaslktes kaqsvvklva
      961 tnliangrtw dgvqllclig kgldacrylq aagqwedsvw lakcilsgda lsevvskwae
     1021 hliqlnqkgk avlallsvgq ytrclqtlht armveraalf ldacmehgll hepsslsqnv
     1081 wldyarylhs ignkraslhf cclagelaad lrkevkmlld eeegriseae p
//
LOCUS       XP_077506060             621 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115492 [Amblyomma americanum].
ACCESSION   XP_077506060
VERSION     XP_077506060.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649934.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..621
                     /product="uncharacterized protein LOC144115492"
                     /calculated_mol_wt=70728
     Region          173..591
                     /region_name="RecD"
                     /note="ATPase/5#-3# helicase helicase subunit RecD of the
                     DNA repair enzyme RecBCD (exonuclease V) [Replication,
                     recombination and repair]; COG0507"
                     /db_xref="CDD:440273"
     Region          246..>412
                     /region_name="DEAD-like_helicase_N"
                     /note="N-terminal helicase domain of the DEAD-box helicase
                     superfamily; cl28899"
                     /db_xref="CDD:475120"
     Site            order(273..279,366)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:350670"
     CDS             1..621
                     /gene="LOC144115492"
                     /coded_by="XM_077649934.1:1..1866"
                     /db_xref="GeneID:144115492"
ORIGIN      
        1 msaqeaawvl lqfhmsetst dvifvntvwp eerirskktk demdrehlea nstdiwresa
       61 idkyeerppe ledltlaefm teysskgklt krtkrailrc rdydindvvn ykrehvmlyv
      121 pfrrenefld rnkfetifde nkerlmevke kfnsgvtsae lleyvknine smgieddera
      181 vaaaegeerg atrakivven ddtdivpena vasalvasnc pavkkredcm pledfyarmr
      241 mtnhrqrmii eevihrlttd dseplrifft gpagcgktft lhlimdvynr yckskkiayg
      301 dteisrvnaf vacattgkaa valngvtvhs afkmvmshsr edkglsaadl stfrtlfkgv
      361 rcviidevsm lssdllrqvd rrlrqiradr mrepfgafdv ilcgdmrqlp pvrssevykr
      421 pksngavfst evmawhnley fpltqvvrqs dvvfsnlltk igdgavledf evelqesrfv
      481 daeeagsrse avrlyysnad vdayndkiak rsqdkvdhpt rdfitgyrsq dekdaaaral
      541 etsgrvetgn lpatialcae kpymllknid itdglvngmv gklklieyda qgepcrvwlr
      601 cpleleswpr qswpsaatde t
//
LOCUS       XP_077506150             143 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115571 [Amblyomma americanum].
ACCESSION   XP_077506150
VERSION     XP_077506150.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650024.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 44% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..143
                     /product="uncharacterized protein LOC144115571"
                     /calculated_mol_wt=15945
     CDS             1..143
                     /gene="LOC144115571"
                     /coded_by="XM_077650024.1:83..514"
                     /db_xref="GeneID:144115571"
ORIGIN      
        1 msfnlkrsmv dpastqhprp trkvkhfask eeladhikks pygelfnipg tllhntlmtt
       61 rteiqqpsqa pasvqrqqat hhsekspkck erqlnkvepp vddleitler plcnlnfgkl
      121 lgmditkadd aqqelpppct avs
//
LOCUS       XP_077506254             243 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115648 [Amblyomma americanum].
ACCESSION   XP_077506254
VERSION     XP_077506254.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650128.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 50% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..243
                     /product="uncharacterized protein LOC144115648"
                     /calculated_mol_wt=25737
     Region          <137..181
                     /region_name="Piwi-like"
                     /note="PIWI domain. Domain found in proteins involved in
                     RNA silencing. RNA silencing refers to a group of related
                     gene-silencing mechanisms mediated by short RNA molecules,
                     including siRNAs, miRNAs, and heterochromatin-related
                     guide RNAs. The central component...; cl00628"
                     /db_xref="CDD:412485"
     CDS             1..243
                     /gene="LOC144115648"
                     /coded_by="XM_077650128.1:38..769"
                     /db_xref="GeneID:144115648"
ORIGIN      
        1 matrgagtqp hskpgigvat gpetccdmgq sarvraiqlp alatvrdflt vslitrtcdt
       61 eagirestsg gpavfpaaaf rlfmtrhtav gtavvccrmv lhkgkttaya cmysspyide
      121 lhrggreaql scvefdgtsr psqycivwdd fsftadelqk lvsipapvyy allaayrarn
      181 hvmskvdvss sssdsfggsa gsvspnpvst wgpsrgsspf ptpctsceed hclmsnaqft
      241 eps
//
LOCUS       XP_077506361             138 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115720 [Amblyomma americanum].
ACCESSION   XP_077506361
VERSION     XP_077506361.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650235.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..138
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..138
                     /product="uncharacterized protein LOC144115720"
                     /calculated_mol_wt=14508
     CDS             1..138
                     /gene="LOC144115720"
                     /coded_by="XM_077650235.1:66..482"
                     /db_xref="GeneID:144115720"
ORIGIN      
        1 matrgagtqp hskpgigvat gpetscdmgq sarvraiqlp alatvrdflt vslitrtcdt
       61 eagirestsg gpavfpaaaf rlfmtrhtav gtavvccrmv lhkgkttaya cmysspyide
      121 lhrggreaql scvefdvd
//
LOCUS       XP_077507029             592 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: rho-related BTB domain containing [Amblyomma
            americanum].
ACCESSION   XP_077507029
VERSION     XP_077507029.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650903.1
KEYWORDS    RefSeq; corrected model; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio            :: 10% of CDS bases
            frameshifts          :: corrected 2 indels
            internal stop codons :: corrected 2 genomic stop codons
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..592
                     /product="LOW QUALITY PROTEIN: rho-related BTB domain
                     containing"
                     /calculated_mol_wt=67575
     Region          32..245
                     /region_name="RhoBTB"
                     /note="RhoBTB protein is an atypical member of the Rho
                     family of small GTPases; cd01873"
                     /db_xref="CDD:133275"
     Site            order(35,71..72,76..77,79,81,98,102..103,105..107,113,116)
                     /site_type="other"
                     /note="putative GEF (guanine nucleotide exchange factor)
                     interaction site [polypeptide binding]"
                     /db_xref="CDD:133275"
     Site            40..47
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:133275"
     Site            order(43..48,103..104,106,160,162,228..229)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:133275"
     Site            70..76
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:133275"
     Site            71
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:133275"
     Site            order(72,105,113,115)
                     /site_type="active"
                     /note="putative GDI (guanine nucleotide dissociation
                     inhibitor) interaction [active]"
                     /db_xref="CDD:133275"
     Site            order(72..73,107,113)
                     /site_type="other"
                     /note="putative GAP (GTPase-activating protein)
                     interaction site [polypeptide binding]"
                     /db_xref="CDD:133275"
     Site            order(73..74,113,116)
                     /site_type="active"
                     /note="putative effector interaction site [active]"
                     /db_xref="CDD:133275"
     Site            103..106
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:133275"
     Site            order(106..107,113..116,119..121)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:133275"
     Site            159..162
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:133275"
     Site            227..229
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:133275"
     CDS             1..592
                     /gene="RhoBTB"
                     /coded_by="XM_077650903.1:251..2029"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 2 bases in 1 codon;
                     deleted 1 base in 1 codon; substituted 2 bases at 2
                     genomic stop codons"
                     /db_xref="GeneID:144116161"
ORIGIN      
        1 merrtrrngr hqerrqedsw pstmnneqpn qelvkcvvvg dtavgktrli caracdtrvt
       61 lsqlltthip tvwaidqyri yrevlqrsle vvdsvsvslr lwdtfgdhdk drrfaygrsd
      121 vvllcfsian pvslrnckvi wypeirqfcp ntpiilvgck ndlrymyrde aylslcrdrs
      181 pffrghhpcv scrfpefgrp lresdllrpe qgrllakeig aayyetsvlt nfgvaevfsn
      241 viraallark qqrfwmtnlk hvqhpavqps vivgwatlga ryetdvsyii sdglgpyfea
      301 kvvqeldmpd tfytimiees plpeaxvqql vvlvhyycan tenvvvehlq pfhlghatad
      361 elfsciedtl sdvrkknmic fysdspnamr svkrklkkev spawltsvna astvhnafaa
      421 gldmfcmeve slvtdvhycf kfatrhadmk ellndpglpq xrvlrhgnkr wltllpsvex
      481 vlksydalta ffskagqprr ssmrhgflls afcdktlrak sfflenaaqi fdrfqtlfqs
      541 kdpllhvfyd emlvlvkflk lqtkllsvaf nvtsllqkcs eilykllqtc sv
//
LOCUS       XP_077507327             502 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116458 [Amblyomma americanum].
ACCESSION   XP_077507327
VERSION     XP_077507327.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651201.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..502
                     /product="uncharacterized protein LOC144116458"
                     /calculated_mol_wt=55740
     Region          26..113
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     Region          422..441
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(426,428,430,432..433,436..437,440,456,458,462..463,
                     466..467,470,484,486,488,490..491,494..495,498)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          <433..499
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          449..471
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          479..499
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..502
                     /gene="LOC144116458"
                     /coded_by="XM_077651201.1:1..1509"
                     /db_xref="GeneID:144116458"
ORIGIN      
        1 mdetvptsat ssgernpvwt nrkeaqlikg yrcmrclwdr qnpdyaderm rrlaldiiav
       61 yldlglngvp dvvgkiqelr dqyakeleke srrrptrhlf yrwvhlerme flrsavtade
      121 reqaqvdvpl lepeqgvprv rpevpdneaa nstqtlepnl ssytgeqasm pvlvpyavep
      181 nlssyageqt smpvlvpyav erepkealsv lhnecsaegr gvvsppaetr dlpgmrgncp
      241 vdcplgslds asftraqqsv pgrrrdtpdd eetgtaflep dlsshagesa gsprqffsvp
      301 apmvpweagr ipdqrqppeg ldpivdgsqy epnvpaaqlf pmetehhviq cgnqtpphvd
      361 tvtgpcssae gpsasteigs pnpetgprcs cavlavpegr dpggqqgttr vkggavyics
      421 ypgcgrrhtk rrhfeahlrs htgekpyecd wancgmkfar sdalsrhyrk htgerrypce
      481 rcgrafyrne hllthqkthr ge
//
LOCUS       XP_077507339             373 aa            linear   INV 25-NOV-2025
DEFINITION  kelch domain-containing protein 3-like [Amblyomma americanum].
ACCESSION   XP_077507339
VERSION     XP_077507339.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651213.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..373
                     /product="kelch domain-containing protein 3-like"
                     /calculated_mol_wt=41555
     Region          12..295
                     /region_name="NanM"
                     /note="N-acetylneuraminic acid mutarotase [Cell
                     wall/membrane/envelope biogenesis]; COG3055"
                     /db_xref="CDD:442289"
     Region          13..62
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          121..168
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          172..223
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          226..270
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     CDS             1..373
                     /gene="LOC144116575"
                     /coded_by="XM_077651213.1:1..1122"
                     /db_xref="GeneID:144116575"
ORIGIN      
        1 mwtvrlegvp evashtavpi ngkiyffdpy ckdmdctvrk sidvyvfdpa tyqwnqlqtq
       61 tlpdgspwhs ddgrtvvayr esaylwssra ffeagsvvyr fdtrtmawss lnasgqlpri
      121 rcgqsaclvg srvylyggwp lygpslpqih fldletmqwh svptngelpe srsyhsatai
      181 germyvwggc nqvsaggvya rslvfldtst ltwvlprvhg rppqgredhs tfvyngelyi
      241 fggvdvdqgr yfrtihkysp ekscwsvltp krsgpsarrf pgccviedrv fifggrgles
      301 narveqqves aidetsavle vgltdmhvln lcptlrtmcl mavidagvhv shlppaikke
      361 mialtsysst sss
//
LOCUS       XP_077507347             422 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116893 [Amblyomma americanum].
ACCESSION   XP_077507347
VERSION     XP_077507347.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651221.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..422
                     /product="uncharacterized protein LOC144116893"
                     /calculated_mol_wt=45670
     Region          30..>84
                     /region_name="JMTM_Notch_APP"
                     /note="juxtamembrane and transmembrane (JMTM) domain found
                     in Notch and APP family proteins; cl41775"
                     /db_xref="CDD:425406"
     Site            order(49..50,52..53,55..61,65..70)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:411983"
     CDS             1..422
                     /gene="LOC144116893"
                     /coded_by="XM_077651221.1:42..1310"
                     /db_xref="GeneID:144116893"
ORIGIN      
        1 maagrshsrr aapdsndtak qpqwqdgdpg gpreeqasgl psawvaaiav nvvlvalflv
       61 fvlycyirsr krakgapfpe ergfetqmdk attqplalea prplawppsa avehrqldlp
      121 visthsasvh rtksvpqlia emsarmfmhe epfdlsgste tpiiegvddd eepelkdlsp
      181 deseeelpse plpafgdalg seplvtlpas rvhqaannga flpgaptspk gilyqvclla
      241 gggrdcvgsl elpleqpvtl aelraafsha pdpvlrelat lsrfvteahr plspaeeaty
      301 vvhqvypsra vmlrdttevp easppfwegp ppssatppvi shdpevnpsl ttkvmptrpw
      361 rkmnnsvhsy vgplgvcqrp dcnqagrvkc idcglasyct srclkadaar hslscyrppw
      421 rt
//
LOCUS       XP_077507350             353 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144117051 [Amblyomma americanum].
ACCESSION   XP_077507350
VERSION     XP_077507350.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651224.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..353
                     /product="uncharacterized protein LOC144117051"
                     /calculated_mol_wt=39502
     Region          124..>220
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     CDS             1..353
                     /gene="LOC144117051"
                     /coded_by="XM_077651224.1:1..1062"
                     /db_xref="GeneID:144117051"
ORIGIN      
        1 mcqpcaitas pdraltspvh ctldrqletp spstsspapa fkqlrppasl cglggcatmr
       61 slnpfcfamq vskpcclyak kssdyfliql pspqcclaiv tecsdviisl lllsgdietn
      121 pgpasletvv telkklsagq stlfaevtdl knqllttdnl isdlsrrisa leghyqtiqs
      181 lrteieglst httqatrqlc dledrideae nqsrrnnlif ynipdpdpse twadseklli
      241 rhcsefldit lnpktidrth rlgrhepdrc rpliakfalf ktkdeilang rkfkntdysv
      301 gedfsrrvrn vrkhlvtfak sktnrfslry ktlfigsrry ifdepsqsvk eip
//
LOCUS       XP_077507357             373 aa            linear   INV 25-NOV-2025
DEFINITION  kelch domain-containing protein 3-like [Amblyomma americanum].
ACCESSION   XP_077507357
VERSION     XP_077507357.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651231.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..373
                     /product="kelch domain-containing protein 3-like"
                     /calculated_mol_wt=41717
     Region          15..295
                     /region_name="NanM"
                     /note="N-acetylneuraminic acid mutarotase [Cell
                     wall/membrane/envelope biogenesis]; COG3055"
                     /db_xref="CDD:442289"
     Region          15..62
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          121..168
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          172..223
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          226..270
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     CDS             1..373
                     /gene="LOC144117209"
                     /coded_by="XM_077651231.1:1..1122"
                     /db_xref="GeneID:144117209"
ORIGIN      
        1 mwtvrlegvp evaihtavpi ngkiyffdpy ckymdctvrk sidvyvfdpa tyqwnqlqtq
       61 tlpngrpwhs ddgrtvvayr esaylwssra ffeagsvvyr fdtrtmawss lnasgqlpki
      121 rcgqsaclvg srvylyggwp lygpslpqih fldletmqwh svptngelpe nrsyhsatai
      181 germyvwggc nqvsaggvya rslvfldtst ltwvrprvdg rppqgredhs tfvyngelyi
      241 fggvdvdqgr yfrtihkynp ekscwsvltp krsgpsarrf pgccviedrv fifggrgles
      301 narveqqves aidetsavle vgltdmhvld lcptlqtmcl mavidagvhv shlppaikke
      361 mialtsysst sss
//
LOCUS       XP_077507425             564 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118316 [Amblyomma americanum].
ACCESSION   XP_077507425
VERSION     XP_077507425.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651299.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 50% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..564
                     /product="uncharacterized protein LOC144118316"
                     /calculated_mol_wt=62938
     Region          209..289
                     /region_name="FH_FOX"
                     /note="Forkhead (FH) domain found in Forkhead box (FOX)
                     family of transcription factors and similar proteins;
                     cl00061"
                     /db_xref="CDD:469596"
     Site            order(213,231..232,235,258..259,261..262,265,271,285..287,
                     289)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410651"
     CDS             1..564
                     /gene="LOC144118316"
                     /coded_by="XM_077651299.1:97..1791"
                     /db_xref="GeneID:144118316"
ORIGIN      
        1 mddsdwtwdg dgfqptkrpk legssstrks krprqkgsgg drsrakprer skhplkknee
       61 cgakrgrrgr drvptkekkk lgssavaqpp pgttmecghv pnteeeppav ynedlledla
      121 reyletikpd lstvvrlkgr dssltshsrr srslgyvdtt krrrassepa krsgrtrtms
      181 spagtctvak lppgplaell pagtivrrpp gklvqlvaqa ihesplqvlr vrhvyealkk
      241 rypyfmfmdk kdettwkssv rhalsqrwfk kvhthppdrn aahgksnfwe inyhnrprgw
      301 ampeeeemss tgfgespept psvwsqdldm eldllpplqs pppqqqactp yqvllspqta
      361 mqrqqppvar pmpthssapt lqsssaepsl msplymlhms ppawctgmde ipvgselgpl
      421 caqekhqeqe avmpqeqslp rlgpsnsqeg tlphpelplp pqfpwppqep plpggptspp
      481 rqeetgpwgt gpgfrprsgr lqlpgwpyvr epvfaaianp rravmmedgt wyilvypafa
      541 endeclvrav whweegtpad aval
//
LOCUS       XP_077507516             706 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118418 [Amblyomma americanum].
ACCESSION   XP_077507516
VERSION     XP_077507516.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651390.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 37% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..706
                     /product="uncharacterized protein LOC144118418"
                     /calculated_mol_wt=76633
     Region          448..554
                     /region_name="Fasciclin"
                     /note="Fasciclin domain; pfam02469"
                     /db_xref="CDD:396845"
     Region          573..694
                     /region_name="Fasciclin"
                     /note="Fasciclin domain; pfam02469"
                     /db_xref="CDD:396845"
     CDS             1..706
                     /gene="LOC144118418"
                     /coded_by="XM_077651390.1:1..2121"
                     /db_xref="GeneID:144118418"
ORIGIN      
        1 mekmiisnps tradflqilq rlsqaslmgl aarglsiqan avylttehna wgmaipaaht
       61 datcqqspai slasgqphvl ptckatshgq qkghagkttk llhryrgpfr llrqvaennw
      121 eiinrvgrrr dvinverlkp chirldaeee addespesdy ggdggqakkl sreaflarps
      181 srellggegt rlpngvrgpf agdairvrfk eegdwsrgrl laalkkrrrr lgattrpaam
      241 twnavpwlla iaaiaasgql trprvedggw qpvlgrttgk asakkgraae srqdipafyv
      301 wlptelldhi hpngpnfaqi qppagfqrsr pnylpaidda ldiatrslqa prsaaygplq
      361 seeesdesag krpygslvql tggkgrdryq eapakrpsae tagcsptssa aaavtslgnr
      421 slrqvadslg agrfldrlgi teiellsltg hdgaytlflp addsvelvsp qlldswrsdr
      481 nsrqsallnh vvpsrlalee mmrggsfptr aggrlnvrrn anavvtvngq rviaadgsgp
      541 rggilhvisg plsptadrdv lttlsdcaky dgfltlatgt gvaellrrgq rtlflpsnda
      601 lskvppeelk vyqknvtalk efltyhvvdg iyyshdlrdg qylksmhndl pirvgvnvdg
      661 cnrrlyeann splyradipa sngvihvvdw illpgdhkwc tgvalp
//
LOCUS       XP_077507590             238 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118522 [Amblyomma americanum].
ACCESSION   XP_077507590
VERSION     XP_077507590.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651464.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..238
                     /product="uncharacterized protein LOC144118522"
                     /calculated_mol_wt=26563
     Region          166..>235
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..238
                     /gene="LOC144118522"
                     /coded_by="XM_077651464.1:482..1198"
                     /db_xref="GeneID:144118522"
ORIGIN      
        1 mlghdylplp stlglgtiag adsavhdtdd phssrapqlt dvqttppppa deegaaqstp
       61 irviasaswd spdyaeagps glsgakesvr qpvmtpasgt pksrrrkgav vytprtkkrf
      121 eklhsrsdry rkalsrirkr hdtrrvtqqe alnklephlp qdlfelvkaq irlcsfpksk
      181 khwskqmkqf alnlffhspk ayrflremlh lphvrtlrrw ladipmtpgi ipgamdai
//
LOCUS       XP_077507660             385 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118632 [Amblyomma americanum].
ACCESSION   XP_077507660
VERSION     XP_077507660.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651534.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..385
                     /product="uncharacterized protein LOC144118632"
                     /calculated_mol_wt=43192
     Region          <2..74
                     /region_name="RT_LTR"
                     /note="Reverse transcriptases (RTs) from retrotransposons
                     and retroviruses which have long terminal repeats (LTRs)
                     in their DNA copies but not in their RNA template. RT
                     catalyzes DNA replication from an RNA template, and is
                     responsible for the replication of...; cd01647"
                     /db_xref="CDD:238825"
     Region          98..>169
                     /region_name="RT_RNaseH_2"
                     /note="RNase H-like domain found in reverse transcriptase;
                     pfam17919"
                     /db_xref="CDD:465567"
     CDS             1..385
                     /gene="LOC144118632"
                     /coded_by="XM_077651534.1:1..1158"
                     /db_xref="GeneID:144118632"
ORIGIN      
        1 mavailqrym kellhgidgv lvflddilig gkdevqhyah lqevlkrihe dglrlkfdkc
       61 qfcveevcyl rfrvnsagaa hvleplcrll dkgrewhwte aeadafcqar gllqsshvlv
      121 hynvnkplvl acdasphglg avlshvgedg rllhhakpqv lsprmlrwtl mlsaydyeie
      181 yrpaelsnad afnclplras kspakllmgr klstvlerlh pdlqsgvqqe qvmqrrnqav
      241 rlfevgdrvf mrnylygpkw lpgivdavtg pvsylvvtkd grqwrrhvdq lrsrsasyal
      301 dsstsgmsee eeplvaslag cselgippsp edpgrrdqge ireeaatpst esgscgedre
      361 vsaslqvqrp qrqhshpayl kdfvm
//
LOCUS       XP_077507863            1203 aa            linear   INV 25-NOV-2025
DEFINITION  structural maintenance of chromosomes protein 3-like [Amblyomma
            americanum].
ACCESSION   XP_077507863
VERSION     XP_077507863.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651737.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1203
                     /product="structural maintenance of chromosomes protein
                     3-like"
                     /calculated_mol_wt=136202
     Region          19..1184
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     CDS             1..1203
                     /gene="LOC144118946"
                     /coded_by="XM_077651737.1:1..3612"
                     /db_xref="GeneID:144118946"
ORIGIN      
        1 mtvdddtalg qkhcclawvt legflsykne atlgplspgi niivgengag ksnllyaiet
       61 lisfetgsla qlqhlavlst apgmrsnvat vkallnnksr hlpqeedvvt vtrtigpsaa
      121 tlkignslvg kkdfidylha agfssentfh iirqgqvrev lqmtpkerlr mvqrfagwdv
      181 llqleklale caelgdrdrk rieeiinecr vklqeddqni keaklykale ldirtvnlal
      241 ldkdadffka tsqqiednia etqalqamak qrhlrlssgl qenkhklkql reevqnrlsr
      301 lddissqlcq vegdkvresl hqnqfqgeis aaeteqqila dsrthshqav kdmtlklnkt
      361 tealhalers edqlksrvee ltnenhavmq cvwhghtfrt tqerniwvdg evqklesilq
      421 diqkqceelq leiesctaer drciseekka qaeldslrql dgsmkvqlmq lpkqryaaan
      481 kyvlaqrlee dcqhalskar ndllriqprm vsamgkdvfn awqglkrlla nrddsdaqey
      541 igmlvdhlrv pddltlavav algihlftpl vrssksatvl lnkfrdlnvp gclgflaldk
      601 ariqeeypdp hgggvrrlld vvqctnadla pllpywlgsw llcpdlevaq eacrkyeanc
      661 vtpegdivrf rgamtggyhd pkkngfavye efcrlsdvlq saesslsess gavrrcaaes
      721 temssklqvl dmkvaqntna issceanlas lrlrlsqied gikakeqalh krletaasyq
      781 hqkkalleer ssdrvehlta saeqkllert qslqrlnerl klireerlkt qhkknieest
      841 lkycllpeld rlnresqale hrkvaleref elskmrlaaa qetkdslenq aaelmnntdd
      901 qscilnlern vqemseeisr slddlgaask hmemlmmkra gtldalkeve kkarmlgvvs
      961 rdlegyrsmd rsqleherqh llarlsrmdp pdlsagrlve easkklddvc rqlqdlvlts
     1021 rmaremtdsq eeqkfndief tlkqvskyfe dlfskfvprg raimvfhkkr qrgerrtdek
     1081 tyesiefkas fsaqgvlqti ksfsggqktv valcfilalq kcdpapfyif devdacldae
     1141 hrqcladvle elssksqfic ttfrpeltsk gkvfkvtqhq gvssiaeitq realqllqar
     1201 gdy
//
LOCUS       XP_077508064             410 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119238 [Amblyomma americanum].
ACCESSION   XP_077508064
VERSION     XP_077508064.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651938.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 49% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..410
                     /product="uncharacterized protein LOC144119238"
                     /calculated_mol_wt=45791
     Region          347..>405
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     CDS             1..410
                     /gene="LOC144119238"
                     /coded_by="XM_077651938.1:4..1236"
                     /db_xref="GeneID:144119238"
ORIGIN      
        1 mdrmlnyqqa tgltdgevla gvvpvsltdq aarwfcltgh rsrtmeefra rfreeflpad
       61 ydrrlrrele lrtqhpdesl leyvramdel yriadpsapn gekvervtrq ahptfaaylr
      121 gvqfrdldkl asaakriqad ilashayrpp ppasqalepr cawngdtfri rsrgaetvaf
      181 sdgqlrngwe lsdraldpyt yamraahaar nqdvrndrpk kpletpsael pyqpvdraer
      241 kssegnpslp vrppvppava lcaaaacrps hpllnnsggl sgeenarlsl llnkydavft
      301 erpgcttlvr hkietgdavp wkcnprpvsl akrkaldnal delldtgvve rsnspwgfpe
      361 vlvpkkdgtt rlcvdyrrln evtwkdaypl psipsivsnv ggakycddrp
//
LOCUS       XP_077508137             149 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119343 [Amblyomma americanum].
ACCESSION   XP_077508137
VERSION     XP_077508137.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652011.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..149
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..149
                     /product="uncharacterized protein LOC144119343"
                     /calculated_mol_wt=16523
     CDS             1..149
                     /gene="LOC144119343"
                     /coded_by="XM_077652011.1:137..586"
                     /db_xref="GeneID:144119343"
ORIGIN      
        1 mwlllmrsct sqddfrmkrg lvsscidlfv sfgkemasdd dlgdlpscpf icakgwtflt
       61 ansyticvdt hpvlhaskpd eafkllffah fafniqyqke tslcleftqr aiaginparg
      121 tkvqknggkq hclsprvaal atalkdydf
//
LOCUS       XP_077508360             160 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119631 [Amblyomma americanum].
ACCESSION   XP_077508360
VERSION     XP_077508360.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652234.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..160
                     /product="uncharacterized protein LOC144119631"
                     /calculated_mol_wt=16741
     CDS             1..160
                     /gene="LOC144119631"
                     /coded_by="XM_077652234.1:91..573"
                     /db_xref="GeneID:144119631"
ORIGIN      
        1 mssdganegd kagavsgthr crvretkkvg vepvlglras slnavdvnqa srslaswrrh
       61 lvptkpseag qrrgsrrpaq qppkpvaapd psvagtesim nvlkrvseiq vtveyidspl
      121 ssiierikpc tpeasddeae rsgagsdsap cdsattaarq
//
LOCUS       XP_077508511             284 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119833 [Amblyomma americanum].
ACCESSION   XP_077508511
VERSION     XP_077508511.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652385.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 36% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..284
                     /product="uncharacterized protein LOC144119833"
                     /calculated_mol_wt=31719
     Region          <154..219
                     /region_name="rve"
                     /note="Integrase core domain; pfam00665"
                     /db_xref="CDD:459897"
     CDS             1..284
                     /gene="LOC144119833"
                     /coded_by="XM_077652385.1:1..855"
                     /db_xref="GeneID:144119833"
ORIGIN      
        1 megpseppae spvrdkyqcp vcqaefvldq srrrhvrkqh peaasslsgs akasyscdqc
       61 tnvfarrgql lqhheqnhgf vsrlsthqfs syydfaawke teemkeqvqf iassgpkrss
      121 sgvtkmyllc srsgmhektg egkrqpklvg svkcnkhcfa tmdisqegen vtveyqaehy
      181 ghdrkmvvsd ngtqfsssdf akfaqeygfk hvtsspehpq sngitedavk imktsmkktq
      241 dpyltllsyr stplkngysp sellmarqlr tknpltpgld tcsg
//
LOCUS       XP_077508699             415 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120102 [Amblyomma americanum].
ACCESSION   XP_077508699
VERSION     XP_077508699.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652573.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 20% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..415
                     /product="uncharacterized protein LOC144120102"
                     /calculated_mol_wt=46704
     Region          1..355
                     /region_name="transpos_IS630"
                     /note="IS630 family transposase; NF033545"
                     /db_xref="CDD:468076"
     CDS             1..415
                     /gene="LOC144120102"
                     /coded_by="XM_077652573.1:142..1389"
                     /db_xref="GeneID:144120102"
ORIGIN      
        1 mtgvsprtvv rfkkeglsgg vkspqkrpkr maisssrsvk ydsftvnair lklhcmyaer
       61 qiptldsilh evngdddlpd mskatlwrll kemgfkfekr krtlaliers divawrrryl
      121 raikafrsqg rcivyldetw vnaghtrehv wkdstvkssq daflrglttg lpapsgkggr
      181 lilvhagssl tgfvsgaadf frakkgasad yhseidgkyf eewfsskllp nipnssiivm
      241 dnapyhsval ekppttstrk ieiqawltrk giswstdmvr aelldlckrt tsghtvyaid
      301 tlaakhghev lrlppyhcef npieqvwsmv kgfvekrnka ftlaeveell pqalahvske
      361 dwqkfcahae riedeawerd iifddevdpv vfaigsssss seessselsg ieele
//
LOCUS       XP_077508757             449 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120196 [Amblyomma americanum].
ACCESSION   XP_077508757
VERSION     XP_077508757.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652631.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 33% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..449
                     /product="uncharacterized protein LOC144120196"
                     /calculated_mol_wt=49896
     Region          <3..55
                     /region_name="Forkhead"
                     /note="Forkhead domain; pfam00250"
                     /db_xref="CDD:459732"
     CDS             1..449
                     /gene="LOC144120196"
                     /coded_by="XM_077652631.1:1..1350"
                     /db_xref="GeneID:144120196"
ORIGIN      
        1 msrnkfpfyr fvdmgaqatw kssvrhalcq cwfikvpssk arnsfwslny serprvwimp
       61 ekeeqgqple ttealtsagd lglmpssart ppnlepprdi imssvattga sqlpmvssqs
      121 qeesstsnsp lpvllprstq atpksspssc npvsptpqyr dtavsnsapa raepapvgrc
      181 igvkdtptvk ypgpfpvqep aprpssappr lgratsqqta lplvvgrsre qhqpqagpqp
      241 qpqlqpprqf vgwphgnaer fyalhvpslq dyaqqemqpp ygrrrpgdny slswdphdrv
      301 gnvlsednfy sgrpygtqsp prpqttihsa rhgpelqwtp tdasfvpllp qresplsrqe
      361 ppsrrerpas pqglsseqdr qsllhedlls eheqpllrsy rpqqqgmprv eldairlngr
      421 pyvvrnarrs addpravtie cyaalerpt
//
LOCUS       XP_077508825             484 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120286 [Amblyomma americanum].
ACCESSION   XP_077508825
VERSION     XP_077508825.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652699.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 15% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..484
                     /product="uncharacterized protein LOC144120286"
                     /calculated_mol_wt=53578
     Region          <55..258
                     /region_name="COG5025"
                     /note="Transcription factor of the Forkhead/HNF3 family
                     [Transcription]"
                     /db_xref="CDD:227358"
     Region          85..151
                     /region_name="FH_FOX"
                     /note="Forkhead (FH) domain found in Forkhead box (FOX)
                     family of transcription factors and similar proteins;
                     cl00061"
                     /db_xref="CDD:469596"
     Site            order(99..100,103,126..127,129..130,139,146..148,150)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410651"
     Region          <211..>311
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          402..470
                     /region_name="Forkhead"
                     /note="Forkhead domain; pfam00250"
                     /db_xref="CDD:459732"
     CDS             1..484
                     /gene="LOC144120286"
                     /coded_by="XM_077652699.1:1..1455"
                     /db_xref="GeneID:144120286"
ORIGIN      
        1 mpdmstvvqa asdvpssagh qhrcgepstk krrrgaawqw tvrrqklpsi edtctvdrtp
       61 pgplgellpa gtclrrppgf lvqhianvih ksprqmlrvq hiyealrnkf pffrfvdkda
      121 hatfkssvrh alfqrwfikv pssnatnsfw slnyserphd wimpkkeeqg rpnettealn
      181 fasdlgpvps partppdlep prdirmssvp ttgasqlpmv ssqsqektst sssplpvlls
      241 rttqatpkss psdsnplspt pqyrdtgvss saparaeptp vgmctglket ptvdcpgplp
      301 vqveeetdal snayvssimp dmstivqaas dvpscagrqh lcdepstkrr rgaglqstfr
      361 rqnqpntedt ctvdrappgp lgellpagtc lrrpsgflve hianaihksp rqmlrashiy
      421 ealrnkfpfy rfvdmdaqat wmssvrhalc qcwfikvpss karnsfwsln yseryayvsl
      481 prav
//
LOCUS       XP_077508869             368 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120369 [Amblyomma americanum].
ACCESSION   XP_077508869
VERSION     XP_077508869.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652743.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 29% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..368
                     /product="uncharacterized protein LOC144120369"
                     /calculated_mol_wt=41751
     Region          5..>42
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..368
                     /gene="LOC144120369"
                     /coded_by="XM_077652743.1:1..1107"
                     /db_xref="GeneID:144120369"
ORIGIN      
        1 mfrpsiilla ivdhqyrflc lsvgspgrch dahvygrskl kkivdsdrfy ftvsviegtp
       61 vapiilecfd ivnqllalsg dvelnpgpnt rhqvqdslpd vqskqledmf amletlntrt
      121 akmhldqkaf iksiddlkks qlklgdlsem nkiltnverk tvtideteke vhpfqqlvdq
      181 lssensqlra rlseledrqr rddllfygip daqseswaqs eeklvkvlss cleipsseil
      241 idrahrlggf asekcrpiia kfssfkvker illncsklkd ekitlsedfs fatrharkkl
      301 vefgksqplt fklrfnklyi knkcysynhs ddavheipsa fplptdrsed ptndthsasa
      361 pptelvtn
//
LOCUS       XP_077508992             639 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120436 [Amblyomma americanum].
ACCESSION   XP_077508992
VERSION     XP_077508992.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652866.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 43% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..639
                     /product="uncharacterized protein LOC144120436"
                     /calculated_mol_wt=70407
     CDS             1..639
                     /gene="LOC144120436"
                     /coded_by="XM_077652866.1:1..1920"
                     /db_xref="GeneID:144120436"
ORIGIN      
        1 mtlrtsmvat larikdavne hcaqsvaggw lvlitallqv aptqrpqiyk pdaiykdead
       61 gitcdtrikd avnehcaqsv agvwlvlitt llqvaptqrp qiykpdaiyk deadgitcdt
      121 rikdavnehc aqsvagvwlv littllqvap tqrpqiykpd aiykdeadgi tcdtrikgav
      181 nehcaqsvag vwlvlittll qvastqrpqi ykpdaiykde adgitcdtri kgavnehcaq
      241 svagvwlvli tallqvlaav vthallpalv sagvecvpsd lpgypcsdth vrclnaisas
      301 nlpasslepw tlpepwthps sstapgtpci kerqspaalc glggctmkqt tnplllimqf
      361 snpyalsakk tsnyclvqlp npqccvdvai ecletirill llskdietnp gpasaeavla
      421 elqklssgqa tiisdaqdik ntlpttekti tdliqriadl eshyqnlatl ktdvecmqcn
      481 tekatqlvrd ledrfdyaen rsrrcnlify glsdptpses fadseeviir hcsdqldlsl
      541 dskdierahr lgrhnterqr piivkfasfk tkqmilsngr klkdtdfsig edfsrrvqni
      601 rkqlvvfaks kttkfslryk tlhigpkryf fyeseqtvk
//
LOCUS       XP_077509371             211 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120610 [Amblyomma americanum].
ACCESSION   XP_077509371
VERSION     XP_077509371.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653245.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..211
                     /product="uncharacterized protein LOC144120610"
                     /calculated_mol_wt=22840
     CDS             1..211
                     /gene="LOC144120610"
                     /coded_by="XM_077653245.1:152..787"
                     /db_xref="GeneID:144120610"
ORIGIN      
        1 mkideddlwr gtsggrlssr asrvvqlvgp vsaggvgrpy cqqlaapcqq qeraspphpl
       61 rpprghrasv gsipgtsglq reahsaagtq qptalptlps spsppsppvp grpvlwrsqq
      121 deevaellre trrtvdlaea rnrrdaefqa rllehlneaa anrrqitatv adlaaavrdt
      181 reqsvrlheq lvvavsflvl mlhyqvhppp s
//
LOCUS       XP_077509606             191 aa            linear   INV 25-NOV-2025
DEFINITION  chitinase-3-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077509606
VERSION     XP_077509606.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653480.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..191
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..191
                     /product="chitinase-3-like protein 1"
                     /calculated_mol_wt=21232
     Region          <19..149
                     /region_name="GH18_chitinase-like"
                     /note="The GH18 (glycosyl hydrolase, family 18) type II
                     chitinases hydrolyze chitin, an abundant polymer of
                     beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a
                     major component of the cell wall of fungi and the
                     exoskeleton of arthropods. Chitinases have...; cl10447"
                     /db_xref="CDD:471972"
     CDS             1..191
                     /gene="LOC144120759"
                     /coded_by="XM_077653480.1:1..576"
                     /db_xref="GeneID:144120759"
ORIGIN      
        1 mhdaqvease aflgelrsrk rvalelslfg yayrlrntgs yelrasvrgp apprpfider
       61 gllvyfeicl efssdlsvpl ynkeadckia vnrdkgqwig ydkatilnnv vfakrmlmgs
      121 iavvtvdmdd yngncgqrnv llgqlhnaln eldrpikrls anlslsesas ssttapaava
      181 qwnvqerymh c
//
LOCUS       XP_077510075            1470 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121016 [Amblyomma americanum].
ACCESSION   XP_077510075
VERSION     XP_077510075.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653949.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1470
                     /product="uncharacterized protein LOC144121016"
                     /calculated_mol_wt=166177
     Region          506..1066
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1470
                     /gene="LOC144121016"
                     /coded_by="XM_077653949.1:1..4413"
                     /db_xref="GeneID:144121016"
ORIGIN      
        1 mapttafcar drednyrema lrvhcldsgw gtptasqlrr llglqcavgq vvtgdcgeva
       61 vyesptwtan vtlqlerspk dsfmhivlkw cdqcqnasnn gaaagksgpp ewwlaseele
      121 efcdpystqi atehkdvpvn afalgcflgy ntfvahfdst ytmppppspl sstkssssls
      181 gksmsvkrgk kgravksssp sdgtsssakt scpnseltss egfkvtqcna hfehcsngly
      241 lyfvdvrkrs stlfrllvry sticriilhk tsptstivyl qllhppllyk vcagnsatsq
      301 cnregdylpq fdlsdttpwd ravefgegpf kcgsdvlgrs rmmrltlsgp rhwaplcnli
      361 lrtrknaqvf yapmrelhar elkavarpsv qdfgvvyall svwsesfqvt delamnaqsc
      421 retllelsar aeecpralee alfeiyfsls qgkvfwfrsa lerlyfryra hrlridvftg
      481 sviaqelpsh mvkvrkvilt ptrrvymppq lvcksrllqh cdpdyalriv vrdddcklis
      541 fslgackddf lsavikppls sglkiggrrf mflgcstsql rshgvwfyah dnqgrtaeqi
      601 rdgigdlsti vsvpkfmarl gqafsqslgl ltvpsqntsv epdirhaapg rssdgyhrey
      661 ifsdgigris hgllrkvhha leleegeepc avqiryggck gmllldptls gcrivfresm
      721 skfhsdhsdl yvlktskprv lylnrpmiti leqsgikaev flmlqnkild sfidsmmdph
      781 eaahvlrsyc alrlpykela gvgidltvep ffralvravn kkvlkelrtk arilvppnyg
      841 rtmfgvldet gtleygqvfv qysndmlryk vndpatileg dvivtknpcm npgdirklea
      901 vnvpqlhhvr dcivfpqkge rphpdemags dldgdeysvl wyedlifnnn cnpmhyhsdp
      961 pkerkasigv qdmvdffcqy ikgdkiglia nahlvwadil dsginshrcr elarkcavnl
     1021 dfakcgdlkg fqnsekppmy pdfmekldtk ntycsrkvlg qlyrnckkve lsteclevve
     1081 eslpdprlll egreqflkea tsaykryakk irallksyri eteseafsga vsklskymke
     1141 ndptdmamvl esqvehvvrr treeffseql deaheklkas awyqvtyelq sseggiqsfp
     1201 wvvsdvlmri vvntssclpe pasrnsfcqr lgalllglph pgggdqdgtq hgdtqvltnl
     1261 lrlmydwids sreflfvknt eelgvyksim reacfkvsrs isgdmpphkl vilclrfaca
     1321 wclkifqggs dgeviskecr rryrlghlal itlnrlsmsg nlaylrrape gcpsteliri
     1381 yinredeeff eilrryedii kkimmdwsgv ediqcdlktd rmdewflqlm vtgsrwaler
     1441 lkeivvypsf revlllafer eknaiggtlq
//
LOCUS       XP_077510314             449 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121151 [Amblyomma americanum].
ACCESSION   XP_077510314
VERSION     XP_077510314.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654188.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..449
                     /product="uncharacterized protein LOC144121151"
                     /calculated_mol_wt=47340
     CDS             1..449
                     /gene="LOC144121151"
                     /coded_by="XM_077654188.1:722..2071"
                     /db_xref="GeneID:144121151"
ORIGIN      
        1 mppkpfavvk fpmeddslav vpvgwlshdw qhchwpkrra aevirlaree adpsapgcew
       61 frcpvavvtt crsyknaekk akkyemssav essaisgneg pdgpdvrlps ppprlpsppp
      121 rlpspppqlp spprlispps llsshsmeaa tpaganctag cpagsastag dledveeats
      181 dptmlkvlrl lleirqqqre ilqrvsrleq arqeprtrsp sppvsslppl cpqlpafsie
      241 dfeaaeaavr ddrvaaalkk qllrlggnnl kevaanvmga vmgvavqrlf slrgrkgkrp
      301 fvgtklcrva tdaicerqgv dilaaqgfig rwlpgacdrg ggrkrryaqa leppeshaqa
      361 ppanpcagsa pcpgapsasc patppgmail spsgvhpssa pptasssrpa tlhpssapsp
      421 apptasssrp atphstlatw viaprahek
//
LOCUS       XP_077510439             149 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121199 [Amblyomma americanum].
ACCESSION   XP_077510439
VERSION     XP_077510439.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654313.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 25% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..149
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..149
                     /product="uncharacterized protein LOC144121199"
                     /calculated_mol_wt=16370
     Region          <2..69
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..149
                     /gene="LOC144121199"
                     /coded_by="XM_077654313.1:57..506"
                     /db_xref="GeneID:144121199"
ORIGIN      
        1 msflmtplad anppntpegr yqaahiktrn sverafgvwk rrfpcldmgl qnrpersavi
       61 itacgalhnl avlrkdpepp avalpviqqp rpsvqpqqlh vppqpsantv ngshvrvgli
      121 aspsstpisg hrwhiigenw reyrpfigi
//
LOCUS       XP_077510670             440 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121335 [Amblyomma americanum].
ACCESSION   XP_077510670
VERSION     XP_077510670.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654544.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..440
                     /product="uncharacterized protein LOC144121335"
                     /calculated_mol_wt=49171
     Region          <90..126
                     /region_name="MULE"
                     /note="MULE transposase domain; pfam10551"
                     /db_xref="CDD:463145"
     CDS             1..440
                     /gene="LOC144121335"
                     /coded_by="XM_077654544.1:1..1323"
                     /db_xref="GeneID:144121335"
ORIGIN      
        1 madnkedlek lmdicgvdvk vhseggagsw avllvtpimr raqqlessse vifvdstasc
       61 dstrssvttq gnvarspklt ynaakllppk apetfmthns aaekaaileh wpearqllch
      121 fyvaqaewqw lapakhgvsp serkslmaif qkimdadskd tletakqdly sqqhkgfith
      181 vvtllereee wvllfrlstk trghntnnys easirvlkdi ilhrtrafnv vtlaeflsvv
      241 wekyffhril dhahsritgh mllyerllkk lpkdteknir cngdghyvvp sgsspdskly
      301 tvysdiglct crsgqqgafc khqalvhkvf ggifpnapll tiearynlgk lalgdncpgi
      361 dffeglhetq vlqnaacska ptdaldcwdt pststapttq ysppesrets taqlmnqvsc
      421 drllkrrrte gnstqpmyke
//
LOCUS       XP_077510889             228 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121494, partial [Amblyomma
            americanum].
ACCESSION   XP_077510889
VERSION     XP_077510889.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654763.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         <1..228
                     /product="uncharacterized protein LOC144121494"
     Region          56..216
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..228
                     /gene="LOC144121494"
                     /coded_by="XM_077654763.1:<1..687"
                     /db_xref="GeneID:144121494"
ORIGIN      
        1 pnyhqtslyh pfsilhflks stslsigqpf saiflpskee ivehlpkqfs narvvvdcte
       61 ialgqpnclq calrvyshyk rgftakymva vtpagmtaym segyggrasd kaifeqngli
      121 dalepisdai madrgllide icahylidli rppfkkqkkq lskgealrtq tiasarvhve
      181 raiqrmktfk ilatkvpwnm vrqlddilmi tagitnlssp iiaahrfl
//
LOCUS       XP_077511001             265 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121564 [Amblyomma americanum].
ACCESSION   XP_077511001
VERSION     XP_077511001.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654875.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..265
                     /product="uncharacterized protein LOC144121564"
                     /calculated_mol_wt=30730
     Region          <11..235
                     /region_name="transpos_IS630"
                     /note="IS630 family transposase; NF033545"
                     /db_xref="CDD:468076"
     CDS             1..265
                     /gene="LOC144121564"
                     /coded_by="XM_077654875.1:1..798"
                     /db_xref="GeneID:144121564"
ORIGIN      
        1 mrctragllf gvapstvsra lqikgwrpyr vrvhqhlspa dkaqrvsfcr seqqrlaadp
       61 grldflmfsd eavfhldgqv nrqctrfwsk nnphfthtra vnsprtvvwc aiwregvvgp
      121 fffedsvtad sylrllkerf lpelveyhmq qrlcifqqdg apahyaskvr qwlneefpgr
      181 wmgrgspnms wpprspdltp cdffvwgyik srvyragslh sverlhehik kefdtisddm
      241 raavfseyah rlqrcidaag ghfed
//
LOCUS       XP_077511105             265 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121634 [Amblyomma americanum].
ACCESSION   XP_077511105
VERSION     XP_077511105.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654979.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..265
                     /product="uncharacterized protein LOC144121634"
                     /calculated_mol_wt=30730
     Region          <11..235
                     /region_name="transpos_IS630"
                     /note="IS630 family transposase; NF033545"
                     /db_xref="CDD:468076"
     CDS             1..265
                     /gene="LOC144121634"
                     /coded_by="XM_077654979.1:1..798"
                     /db_xref="GeneID:144121634"
ORIGIN      
        1 mrctragllf gvapstvsra lqikgwrpyr vrvhqhlspa dkaqrvsfcr seqqrlaadp
       61 grldflmfsd eavfhldgqv nrqctrfwsk nnphfthtra vnsprtvvwc aiwregvvgp
      121 fffedsvtad sylrllkerf lpelveyhmq qrlcifqqdg apahyaskvr qwlneefpgr
      181 wmgrgspnms wpprspdltp cdffvwgyik srvyragslh sverlhehik kefdtisddm
      241 raavfseyah rlqrcidaag ghfed
//
LOCUS       XP_077511215             265 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121701 [Amblyomma americanum].
ACCESSION   XP_077511215
VERSION     XP_077511215.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655089.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..265
                     /product="uncharacterized protein LOC144121701"
                     /calculated_mol_wt=30730
     Region          <11..235
                     /region_name="transpos_IS630"
                     /note="IS630 family transposase; NF033545"
                     /db_xref="CDD:468076"
     CDS             1..265
                     /gene="LOC144121701"
                     /coded_by="XM_077655089.1:1..798"
                     /db_xref="GeneID:144121701"
ORIGIN      
        1 mrctragllf gvapstvsra lqikgwrpyr vrvhqhlspa dkaqrvsfcr seqqrlaadp
       61 grldflmfsd eavfhldgqv nrqctrfwsk nnphfthtra vnsprtvvwc aiwregvvgp
      121 fffedsvtad sylrllkerf lpelveyhmq qrlcifqqdg apahyaskvr qwlneefpgr
      181 wmgrgspnms wpprspdltp cdffvwgyik srvyragslh sverlhehik kefdtisddm
      241 raavfseyah rlqrcidaag ghfed
//
LOCUS       XP_077511320             334 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121773 [Amblyomma americanum].
ACCESSION   XP_077511320
VERSION     XP_077511320.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655194.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..334
                     /product="uncharacterized protein LOC144121773"
                     /calculated_mol_wt=37519
     CDS             1..334
                     /gene="LOC144121773"
                     /coded_by="XM_077655194.1:1..1005"
                     /db_xref="GeneID:144121773"
ORIGIN      
        1 mdlgpstslq gdepaagale cvedcrssts dvkvlrcnll dgkaaelwvr qystntntsw
       61 icekiqtkct rmvfhkvwrc qhnprdkssh rntscpakld ikikkvnrdt mkndlflcrq
      121 tplqavvkvs qhnhslqsad slrvlrpsee traafqeyfe dgmttaqais hhervltekp
      181 nghtllansm anppprsvyr whqewrsqky gpqgnpslkl qenkprylaq gtdvsitsva
      241 ddswavlvvt pimhraqqle sardiifvds tsscdetrst itvlltatkt gavpialvih
      301 ssqtregykq afqqlksqhp lcfrnspvcc fiel
//
LOCUS       XP_077511415             706 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121841 [Amblyomma americanum].
ACCESSION   XP_077511415
VERSION     XP_077511415.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655289.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 16% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..706
                     /product="uncharacterized protein LOC144121841"
                     /calculated_mol_wt=75422
     CDS             1..706
                     /gene="LOC144121841"
                     /coded_by="XM_077655289.1:1..2121"
                     /db_xref="GeneID:144121841"
ORIGIN      
        1 maevrscdfp vvrvpdeyfc gdserkgral yesghvhdva avwdnpvrir vkcvaqtsvt
       61 kpsyevelva veialsveaa sknvtecrss satqedfqkf saeeslardk dnpgraarga
      121 swsgadeggc pelrlvarns rrhgehrenv cglpaatsaa ypsspssvgl ayeamgptpl
      181 gfcwpvsspq ffgggrrpfe vaggvrdaln lgrflglsss qllecssprv pylasvvnss
      241 pdatlllsgl htvepfsatn gqpidnasrs aqflhghgpy lqaswlslsr lnagtapvhp
      301 ahawtcakka arsgapfsgl gstpamvran vlrflvqflg lsssqllecs sprvpylasv
      361 vnsspdatll lsglhtvepf satngqpidn asrsaqflhg hgpylqaswl slsrlnagta
      421 pvhpahawtc akkaarsgap fsglgstpam aranvlrflv qasvpdfsld dgncsckagp
      481 ggrckqvaal vqyinreesa sctsgpgvwg kpsvrslgty kkgaclegff gktvediepm
      541 tpavpppqda tavsalldel wdhncllvta ikhelsvtid kepveetfvy gppvvqfnlr
      601 kpglyccqat sensdedmsy ymanivqtae qiaetkkntr grktfcngkk cghaasqpav
      661 ghttylnqek spmywlssfc pnghfghhlq pmgmplkkpy shflgk
//
LOCUS       XP_077511510             251 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121911 [Amblyomma americanum].
ACCESSION   XP_077511510
VERSION     XP_077511510.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655384.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..251
                     /product="uncharacterized protein LOC144121911"
                     /calculated_mol_wt=27738
     Region          <60..100
                     /region_name="THAP"
                     /note="The THAP domain is a putative DNA-binding domain
                     (DBD) and probably also binds a zinc ion; smart00980"
                     /db_xref="CDD:214951"
     CDS             1..251
                     /gene="LOC144121911"
                     /coded_by="XM_077655384.1:1..756"
                     /db_xref="GeneID:144121911"
ORIGIN      
        1 mqastttaap qvaallptsg resrrlplts vflrrgffcl ccffdeirfv rrtfiatgar
       61 rkdrtlwelt khsricsnhf vngekcndpr spaylptlfp seyrctktps vdiyerfrsr
      121 eqsreptssc vqepaadeeg ydstngliiv eiqtdpcawt lepdmqslfl ccmqdssadt
      181 qatfpskket kdaawgtsyg vkastcdpan idslkstsgf rgypsvsacs iekasdilks
      241 iagvslpvfs k
//
LOCUS       XP_077511609             465 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase makorin-3-like [Amblyomma americanum].
ACCESSION   XP_077511609
VERSION     XP_077511609.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655483.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 12% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..465
                     /product="E3 ubiquitin-protein ligase makorin-3-like"
                     /calculated_mol_wt=50786
     Region          110..128
                     /region_name="zf_CCCH_4"
                     /note="Zinc finger domain; pfam18345"
                     /db_xref="CDD:465719"
     Region          <237..384
                     /region_name="PHA03096"
                     /note="p28-like protein; Provisional"
                     /db_xref="CDD:222981"
     Region          293..352
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(295,298,319,321,324,327,345,348)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     CDS             1..465
                     /gene="LOC144121975"
                     /coded_by="XM_077655483.1:1..1398"
                     /db_xref="GeneID:144121975"
ORIGIN      
        1 mwcnrarhhs sfrpplrpha vslpqghpcp rwqqlalrhh pkkrpsgpqa gihgsrtgss
       61 alrrlplngk maeggsveer cgclaggvcr ngrgcpharc hptrrgnnvc qfylsgrciy
      121 gsrcwynhgq rsgagslpsg gsehsqdaag ydglrlgpgy grsaaarapf agplparrpn
      181 ytgparggga vmpratswad apefyprgws tpasagsaqr tddpppskra slppppidss
      241 gagsscvqhe lcdlcqtpsl hphdeeqrrq hreqcarrkg edrtpagqrg egktcgicmd
      301 vvmekepase qrfgllercn hsfcltcirr wrnckefdne vvmacpmcrr pssfvtpsss
      361 wvdmgeekdk llaaykvams qkpcryfkeg rgycpfagsc lyrheypdgs saagsrprlr
      421 sfqssdsdta dspylwdild vvdspqfanf ylgleaegas ddsdp
//
LOCUS       XP_077511812             433 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122117 [Amblyomma americanum].
ACCESSION   XP_077511812
VERSION     XP_077511812.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655686.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..433
                     /product="uncharacterized protein LOC144122117"
                     /calculated_mol_wt=47762
     Region          58..196
                     /region_name="PTZ00184"
                     /note="calmodulin; Provisional"
                     /db_xref="CDD:185504"
     Region          287..426
                     /region_name="PTZ00184"
                     /note="calmodulin; Provisional"
                     /db_xref="CDD:185504"
     CDS             1..433
                     /gene="LOC144122117"
                     /coded_by="XM_077655686.1:157..1458"
                     /db_xref="GeneID:144122117"
ORIGIN      
        1 mgdsvkekkk kskkesepdk apaaapaaaa paaapaaapa ksstkkraqr stsnvfamft
       61 qhqvqefkea fqfidqdkdg fiskndirat fdslgrltnd nelesmisea pgpinftmfl
      121 tifgdritgv ddeevivnaf nlfdqgngmc sedrlrqmlt tfgeklndqe andafaeapv
      181 dsagnvdlkk fasiltkgve eeegavhflp llhlkkkmpk prslscdela plaeklddel
      241 hlapppgpvs qgslrrgrqr aereskrgks dstkrraqra tsnvfamfeq nqiaefkeaf
      301 qmidsnkdgf vckndlratw dslgriipek dldgmiaeat gpinftmflt ifgervsgtd
      361 pedvirnafk tfdksgtgki sekelkkalm twgekfqese vdevlkeapi drdgnidins
      421 yvkiicggtd dee
//
LOCUS       XP_077511927             166 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122288 [Amblyomma americanum].
ACCESSION   XP_077511927
VERSION     XP_077511927.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655801.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..166
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..166
                     /product="uncharacterized protein LOC144122288"
                     /calculated_mol_wt=18575
     Region          108..>166
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..166
                     /gene="LOC144122288"
                     /coded_by="XM_077655801.1:1..501"
                     /db_xref="GeneID:144122288"
ORIGIN      
        1 mspemfdtll sfvtddltrq yvvreplepg erlaitlsyl akgqdikdia layrvgieta
       61 rvtihfccri iwarledqfm kvpsegdwve iakgfkkrwq fpsclgavdg khvaitalan
      121 sgsayfnykh tfsivlmtii dscykyvlid vgaegrqsnd gvlkns
//
LOCUS       XP_077511932             134 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122448 [Amblyomma americanum].
ACCESSION   XP_077511932
VERSION     XP_077511932.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655806.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..134
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..134
                     /product="uncharacterized protein LOC144122448"
                     /calculated_mol_wt=14742
     CDS             1..134
                     /gene="LOC144122448"
                     /coded_by="XM_077655806.1:279..683"
                     /db_xref="GeneID:144122448"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrppfa altsvlccqr pasigpcvlp afgcvippdp
       61 fmlktkshme vacdfcqgpg agweisgwsv vfthqdknhf swlmgdvsrn lrpgqhwtac
      121 isklkkkhet vnky
//
LOCUS       XP_077512000             400 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122881 [Amblyomma americanum].
ACCESSION   XP_077512000
VERSION     XP_077512000.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655874.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 14% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..400
                     /product="uncharacterized protein LOC144122881"
                     /calculated_mol_wt=45238
     Region          183..345
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..400
                     /gene="LOC144122881"
                     /coded_by="XM_077655874.1:1..1203"
                     /db_xref="GeneID:144122881"
ORIGIN      
        1 mveaadlevv ieaakcqtds vkrrllfahg lliaatvacd gtvqrerwmy nrnerwfedt
       61 lphlgerffk qsfrvtpptf ryiveacrpl lqrvdtnmrl aisvekrvav amyklcssae
      121 drsvanlfgl grstvntiyr efcdavvcll edkwvnmpsp damgghiref gavcdfpqav
      181 galdgchfpv sppkesapdy ynykgwhsiv llalvdhryr fryvnvgspg rchdayiyqr
      241 sqlaefiegp tfqapavvfs gtavpplilc dqafpltthl ikpfshsahl dadmkrlnyh
      301 lskarrvven afgrlkarfr fcmkrmecsm dtaplvirac ctlnniceaf gdqvmqdwtp
      361 daqvsealyq qpqrvtddqt aigaavraai leyykqraqn
//
LOCUS       XP_077512131            1961 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC144123072, partial
            [Amblyomma americanum].
ACCESSION   XP_077512131
VERSION     XP_077512131.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656005.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         <1..1961
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC144123072"
     Region          1299..1959
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..1961
                     /gene="LOC144123072"
                     /coded_by="XM_077656005.1:<1..5886"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:144123072"
ORIGIN      
        1 vghhggfhav tgraaqpavp rtkqfvmpgk rafageptte fsmmeiadvn ldslrskrpl
       61 aetvppaepe vnepfrlepf tvhwrsalgr kdaaldtkrp gfwlgangtl svtpnlvrrf
      121 mgrrgppvvs ttlaavpssg vvgisninin airperntsy rsadnssagv dagdrvtsta
      181 dsvntsaggv dlssssegaa vpfikwegap evpdnksqts kvgiftgava hpaglvrgks
      241 dvipdtteqs lemvsdihdl fnlsgdnqsg aahnkekeds slfgspssss tsetthgvgs
      301 qptvsanrlq dkaagdiert frsspgagav ssleglshst ehavsaaage vgrlnesmpa
      361 ngtpsiftel tgeeiaavkn nsenleprlk gtlssepaah tfnvsevpts gvsaaaertf
      421 vrlnesnvla hfsnkpsisy esgapfttss lsrefgagsl smpaggfspr svisnadinl
      481 pafstsgtvr kvykniaese atprsgrats snissanles qsstvtkgre pkvppapstg
      541 eknrdraild ignfssgges alkpsterlg gaesttarin netsasagng aslfgkater
      601 tedyvpyqny sqfppgmlsn atvgalknds nvapnkysll svsstsitna esplssthgt
      661 esnvkndggl lvvgkvpega dkvnvsaltv fpkgagetss rptsaandss svakaingtk
      721 leegtetpsd qnnvthdyft vwpfaategv negkarseqk ateehslkha aqgsvpggsg
      781 kpeesngtss gisseiaeif psgsthtphd lldakssaei rptgdtrevg lskgysmgss
      841 gqdgnetqea avsskadiss rrsthspget dettvaqist lrtrrkirms rrgryrrrst
      901 atattvdatt tsqstvette haqnrypsrk rlkkkrhtrp tsaaklssef asadaskayl
      961 nstpsteraf nasdvageea arnttenetg gatgsykqni kavavthrhd phkkqnvsvr
     1021 asqgtmtpsr sarsqagtdd atndlglfsd qtndtdaldg tyqhagmnlw eipnsasglq
     1081 kehpshkrka rprdsrrlpp kampkhialt rlpytvpiew taerkrsvrt lpststlnps
     1141 ntsallpnlt egswsaspak rerdespepv netqeapnts aafaptiktf nnglsvvgvn
     1201 itevfsedas assepaikda qptsressge qtndsattvg kfasndssei sqspvpteps
     1261 ttpptlvpfd dsvpvvrcgt adcmeeggrl haftdlsqkp ctnfyefscs gwvrthpyps
     1321 nrrkisvddl ivqvteqktm eyiyenaldh atytdpllhn lvklfqgcsd ksflmknpal
     1381 alqtalddvr ltdwpydeep mdleipevma faarklavhp ffapsletds ttgsgwifvl
     1441 keprpvvaed aylagdvask yqkhvtksms lvnsdkniqa lsepvldvdq evykfveknr
     1501 rftdytkehh rkglqhldkr refdvgsfls tlldgiavld sevefviptq sfledvqnit
     1561 mafeknvlln yigfrvvlsl apllphndgr dlahllfare iptrtpakrw rfclrlmenv
     1621 yklpllklqv dsfekkdtfh tinrtmellk khffrtarns srlgqrprei vilklrglrv
     1681 qsssshligd pevrerhyhg vpdvdpsnvl tdyiqtltiv lknywsyygn mgpqfrwhgs
     1741 vfdtvatynh fentlyipps lfmedvkynt fhsplyfprs grrimealyg mleregsffg
     1801 lhnevvkssn wdyattdgyl slrgclkeqf rraamphlvp glmsavhpsr plrqdvrdsa
     1861 ivnivfeafk etmldfgykp gvfvlpgfae tsmdqlffll ygtsqcelss qeaalsdevi
     1921 naahprryrl nnalqnngaf srafmcgngt dmnpknkckv w
//
LOCUS       XP_077512201             166 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123169 [Amblyomma americanum].
ACCESSION   XP_077512201
VERSION     XP_077512201.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656075.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..166
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..166
                     /product="uncharacterized protein LOC144123169"
                     /calculated_mol_wt=18467
     Region          <40..94
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..166
                     /gene="LOC144123169"
                     /coded_by="XM_077656075.1:1..501"
                     /db_xref="GeneID:144123169"
ORIGIN      
        1 mylkatrcqt detahhvdrt gdlgsasryg knidsfraek rnakvyetia qglarsgink
       61 srkqvqskid nltqayrill haassyhspf paysgayvqi tnelncfrnc akqrttgsap
      121 vawiyfseih rflgslpand hslvdesmtv eqvgssffrh cinacn
//
LOCUS       XP_077512280             241 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123266, partial [Amblyomma
            americanum].
ACCESSION   XP_077512280
VERSION     XP_077512280.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656154.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on the carboxy end.
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..>241
                     /product="uncharacterized protein LOC144123266"
     Region          9..106
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..241
                     /gene="LOC144123266"
                     /coded_by="XM_077656154.1:149..>871"
                     /db_xref="GeneID:144123266"
ORIGIN      
        1 mwksrgktcs vfdckncdad lkvwnvsvce thgpqlhedc pctrpfamhr fpqgeknklv
       61 rqhwianlqr kdfnpgssar vcsvhfidgr pskdnpyptl hlgtptahhk grrklvrhdv
      121 kplepetcst aeplshhlsd eqdeesaaad dllpiniate dishrregae slvdgclacg
      181 crckitetse kgtqvepaaa hdhlyattpr qapvtadsac qtifsaystl apshfrfftg
      241 l
//
LOCUS       XP_077512444             254 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123468 [Amblyomma americanum].
ACCESSION   XP_077512444
VERSION     XP_077512444.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656318.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..254
                     /product="uncharacterized protein LOC144123468"
                     /calculated_mol_wt=27885
     CDS             1..254
                     /gene="LOC144123468"
                     /coded_by="XM_077656318.1:1..765"
                     /db_xref="GeneID:144123468"
ORIGIN      
        1 mnqpsdagsp tvavldvklp pfwtgdpelw fvqvesqfaa rritadstky hhvvgslppa
       61 tasevrdlll tppaenayqt lketlirrvt pteperlqql lreaelgdrr psqllrhmqq
      121 laggttasdg rlvrelflqr lptsvriglt asgetdlakm aeladklmav avptvasvha
      181 dapsanflqe mreeisrlad tvaalhssgn qrprqrtasq pqqrrvcwyh rkfgnaarnc
      241 vppcensgna pgqr
//
LOCUS       XP_077512538             405 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123569 [Amblyomma americanum].
ACCESSION   XP_077512538
VERSION     XP_077512538.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656412.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 50% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..405
                     /product="uncharacterized protein LOC144123569"
                     /calculated_mol_wt=44672
     CDS             1..405
                     /gene="LOC144123569"
                     /coded_by="XM_077656412.1:895..2112"
                     /db_xref="GeneID:144123569"
ORIGIN      
        1 miaavsgepv skgyvqsrss qslllfqeda dgasptgstl nqacshasgf imvqsdhtya
       61 gpsteasass lepgtagvaa tlsasadlsp gapfssspls yegsftspin errplqklra
      121 klrqlrnrnk rlqglllqwr rmkttaelvd slrehltpav aafveaqlkm hnvsrfgrrw
      181 csqnktfalg lyfhrpkgsr ycrkllklps eakdavlaar glsvkggyca iidpyrkeik
      241 vkihwvpfhi psetprrals efgevveirh eewnvpgfes adsttrvasm vlkagvtaee
      301 lphlfkfhgg svhvvvpgra payircrrrg hihgdcqtpr ctkcrafghv redcmrtyan
      361 etgaasedcd dkedimdmrk lnrrplmtvv rrarvvnkva svtet
//
LOCUS       XP_077512729             584 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123847 [Amblyomma americanum].
ACCESSION   XP_077512729
VERSION     XP_077512729.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656603.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..584
                     /product="uncharacterized protein LOC144123847"
                     /calculated_mol_wt=64639
     CDS             1..584
                     /gene="LOC144123847"
                     /coded_by="XM_077656603.1:62..1816"
                     /db_xref="GeneID:144123847"
ORIGIN      
        1 mspeqslsss ssrdagsska askddtshag aedpghqeqr pegdgvafer gdtsmflsal
       61 rfgepgcrtg tdpeesaeee nweldiapgn sgyipsghly lqrvqhqsrr trrrmhmeqa
      121 edykrreaer lltenmresl egdkrqvtae eesehprpqt sfvpvpieei eartpwrcds
      181 pqfgradaal ktdypirtdp eesaeeenwe ldiapgnsgy ipsghlylqr vqhqsrrtrr
      241 rmhmeqaedy krreaerllt enmreslegd krqvtaeees ehprprqtsf vpvpieeiea
      301 rtpwrcdspl fgradaalkt dypirtdpee sveednwelg dfralgwsfa mggpltpapq
      361 gtlpwlpygt ridsatnvlm pvqpapghqd nastmiyaht semaailctp ackldhapkk
      421 kkffsyrgpa krvvlwywhy wlpifpqaaa qqsedagvya eggalcdapl esfaiiaeql
      481 rehtvsksgv stpvgpppea taggwqsavl pepasndkhv ppppassdgs cpassedpgg
      541 sggsrvcrrr yggcipagrm dqggacrpgh yynpakargg rqgv
//
LOCUS       XP_077512954             224 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124143 [Amblyomma americanum].
ACCESSION   XP_077512954
VERSION     XP_077512954.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656828.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..224
                     /product="uncharacterized protein LOC144124143"
                     /calculated_mol_wt=24474
     Region          <115..182
                     /region_name="TesA"
                     /note="Lysophospholipase L1 or related esterase. Includes
                     spore coat protein LipC/YcsK [Cell cycle control, cell
                     division, chromosome partitioning, Lipid transport and
                     metabolism]; COG2755"
                     /db_xref="CDD:442045"
     CDS             1..224
                     /gene="LOC144124143"
                     /coded_by="XM_077656828.1:1..675"
                     /db_xref="GeneID:144124143"
ORIGIN      
        1 mlslaepnlt lpvpclvygc trrsrpahsa saattpcalq tlrgastcca qisvysvrgr
       61 qrpleaqhks agdlrqhqgp hpaaatrrgp cgsrgegvlp rwfitadaea srrwlnrrhq
      121 llnrrltatl krmpsvfiln pdhrfldaak apkkhlfagd gyhlsagegv eelaeilvrs
      181 lekvygpgil asfsrvptrh iiyachhcgt kghvngdcye ccrp
//
LOCUS       XP_077513039             128 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124243 [Amblyomma americanum].
ACCESSION   XP_077513039
VERSION     XP_077513039.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656913.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..128
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..128
                     /product="uncharacterized protein LOC144124243"
                     /calculated_mol_wt=14478
     CDS             1..128
                     /gene="LOC144124243"
                     /coded_by="XM_077656913.1:348..734"
                     /db_xref="GeneID:144124243"
ORIGIN      
        1 mpsspasstl ehdlqaedie vhestklpfp gdqetathsa driatpepla sdlehasthq
       61 allnrisqle rlheesqrkl gaakrrylks eeekerlksk fkhifsddqm rsmerlsaqg
      121 mrweatvc
//
LOCUS       XP_077513233             178 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124428 [Amblyomma americanum].
ACCESSION   XP_077513233
VERSION     XP_077513233.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657107.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..178
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..178
                     /product="uncharacterized protein LOC144124428"
                     /calculated_mol_wt=19751
     Region          86..>177
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..178
                     /gene="LOC144124428"
                     /coded_by="XM_077657107.1:1..537"
                     /db_xref="GeneID:144124428"
ORIGIN      
        1 mreaipvekr vaiglyrlrs saedqtiahl fgvgrstvnl ltkeffaavi dvlggkwlnm
       61 vsrqempnhi refyafsgfp qalgamdgch ipisppqeha adyynykgci illaivdrky
      121 sfrylnvgsp grchdafvyg rsqlskhiks dyfqspvsvi egvavppvvl cdqafplt
//
LOCUS       XP_077513342             378 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124483 [Amblyomma americanum].
ACCESSION   XP_077513342
VERSION     XP_077513342.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657216.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..378
                     /product="uncharacterized protein LOC144124483"
                     /calculated_mol_wt=41750
     CDS             1..378
                     /gene="LOC144124483"
                     /coded_by="XM_077657216.1:209..1345"
                     /db_xref="GeneID:144124483"
ORIGIN      
        1 mqvhspsiiw lmlnavfcat wataierapl rgaadgrtlc aattnppiqv nltlsadwea
       61 pssattltvp sikeqrpsta pcglggcatt rlhnplvfam qvhspsisih kkktnyflvq
      121 lpspqcclat ciecfhvvrs lllmcgdies npgpdkldvi lgelkkmstg qtelvkevqe
      181 lkgqllsidl kmtnlttrls alethyesls tlrndlegiq atsagvlear lddaenrsrr
      241 nnllfyglpd tnptetyslt edliirhcsd hldtqlspnn ierahrlgrh skdrkgpiiv
      301 kftsfktkes ilskgskikg tdysvgedfs rrvqncrkhl vafaraksvp fslrfktlhi
      361 gpksyvfdel sesvreip
//
LOCUS       XP_077513649             292 aa            linear   INV 25-NOV-2025
DEFINITION  nuclear hormone receptor HR96 [Amblyomma americanum].
ACCESSION   XP_077513649
VERSION     XP_077513649.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657523.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..292
                     /product="nuclear hormone receptor HR96"
                     /calculated_mol_wt=33300
     Region          96..268
                     /region_name="NR_LBD_F1"
                     /note="Ligand-binding domain of nuclear receptor family 1;
                     cd06929"
                     /db_xref="CDD:132727"
     Site            order(101..102,106,140,144,147,181)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132727"
     Site            order(111,114,118,123,128..129,131..132,135..136)
                     /site_type="other"
                     /note="coactivator recognition site [polypeptide binding]"
                     /db_xref="CDD:132727"
     CDS             1..292
                     /gene="Hr96"
                     /coded_by="XM_077657523.1:1..879"
                     /db_xref="GeneID:144124668"
ORIGIN      
        1 mssptpsdqt agpsgskads cssedlesss sdssqlpwrr wpqcgssqav hdrairsqfy
       61 rvpgtsevlt daemhriqel atanqvlkvp lacdpepslv dvinmtdhai rrlikmskri
      121 aafkslcqed qvallkggst elmllrsvms ydaerdcwkg pdprlmsirv dilkeargnv
      181 yeehkrfina frpewrlden imlllsaitl ftperpnlvh rdvvmfeqdt ylyllrryld
      241 trysgcesrs vflqlirnle dmrtlnenqv silvdlnphe vepllieifd lk
//
LOCUS       XP_077513863             146 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124808 [Amblyomma americanum].
ACCESSION   XP_077513863
VERSION     XP_077513863.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657737.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..146
                     /product="uncharacterized protein LOC144124808"
                     /calculated_mol_wt=16881
     Region          5..25
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          33..53
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(38,40,42,44..45,48..49,52,66,68,72..73,76..77,80,94,
                     96,98,100..101,104..105,108)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          61..81
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          85..>146
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          89..109
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          117..134
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..146
                     /gene="LOC144124808"
                     /coded_by="XM_077657737.1:142..582"
                     /db_xref="GeneID:144124808"
ORIGIN      
        1 myfwcelcgk vfarcqavls hcrrshgirr fvctycgaaf lqrvhltkht rthtgerpfp
       61 cqmcqshftq kvhlvehmrt htgerpyrcd icqatftqka hlvghtrtht verpfqgpac
      121 pkafatssal kqhryvthrn tendnn
//
LOCUS       XP_077513967             145 aa            linear   INV 25-NOV-2025
DEFINITION  protein Skeletor, isoforms B/C-like [Amblyomma americanum].
ACCESSION   XP_077513967
VERSION     XP_077513967.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657841.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..145
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..145
                     /product="protein Skeletor, isoforms B/C-like"
                     /calculated_mol_wt=16179
     CDS             1..145
                     /gene="LOC144124877"
                     /coded_by="XM_077657841.1:1..438"
                     /db_xref="GeneID:144124877"
ORIGIN      
        1 myfppfsqgh rllagvvlda rnkvdvskgt grycewthkt vdksdesstw edfkqslnlq
       61 cdvgqpgtft wtldkdtpdv vyyqcfthyy lgwkimvtdp dqpergrgkd rmesgrgedr
      121 mesgagptta llpllllgvi vskaa
//
LOCUS       XP_077514083             223 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124951 [Amblyomma americanum].
ACCESSION   XP_077514083
VERSION     XP_077514083.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657957.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..223
                     /product="uncharacterized protein LOC144124951"
                     /calculated_mol_wt=25488
     Region          53..169
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..223
                     /gene="LOC144124951"
                     /coded_by="XM_077657957.1:1..672"
                     /db_xref="GeneID:144124951"
ORIGIN      
        1 mggsmttfdt dadkwqqhvm riedelalel lrtaehmrec yavtgfpqai galdgchfai
       61 sppkkdaagy ynykgwhsti llaivdhqyr flylnvgspg rchdahvygr sklkrivdtl
      121 fnynlstsrr ivenafvrvk arfrfimkri eckpsnakqa tkasctlhni ceafrdnvde
      181 ewvedmhtfn alyqqpshns dactgdgqdv raplaeyfwk raq
//
LOCUS       XP_077514205             210 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125014 [Amblyomma americanum].
ACCESSION   XP_077514205
VERSION     XP_077514205.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658079.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..210
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..210
                     /product="uncharacterized protein LOC144125014"
                     /calculated_mol_wt=23535
     Region          124..>186
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..210
                     /gene="LOC144125014"
                     /coded_by="XM_077658079.1:1..633"
                     /db_xref="GeneID:144125014"
ORIGIN      
        1 mggsmttfdt dadkafrvsp atfryevell qsvlqrqvtn mrkpitvekr vavglyrlcs
       61 saedrtiahl fgigrstvnt vsrefcqavv nnlestwldm prqmaehmre cyavtgfpqa
      121 igaldgchfa isppkkdaad yynykgwhsi illaivdhqy rflylnvgsp grchdahvyg
      181 rsklkrivds drfnppvsvi egtcctnysv
//
LOCUS       XP_077514327             163 aa            linear   INV 25-NOV-2025
DEFINITION  protein Skeletor, isoforms B/C-like [Amblyomma americanum].
ACCESSION   XP_077514327
VERSION     XP_077514327.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658201.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 18% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..163
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..163
                     /product="protein Skeletor, isoforms B/C-like"
                     /calculated_mol_wt=18140
     CDS             1..163
                     /gene="LOC144125085"
                     /coded_by="XM_077658201.1:1..492"
                     /db_xref="GeneID:144125085"
ORIGIN      
        1 maeqfgtpsv spavqearfg fehaakghrr lagvvldirk evdvskgtgr ycewthktvd
       61 ksdesstwed fkqtlnlqcd vgqqgtftwt pdkdtpdvvy yqcfthyylg wkimvtdpdq
      121 pergrgkdrm esgrgedrme sgagprtall pllllgvivs kaa
//
LOCUS       XP_077514448             228 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125154 [Amblyomma americanum].
ACCESSION   XP_077514448
VERSION     XP_077514448.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658322.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..228
                     /product="uncharacterized protein LOC144125154"
                     /calculated_mol_wt=25706
     Region          142..>204
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..228
                     /gene="LOC144125154"
                     /coded_by="XM_077658322.1:1..687"
                     /db_xref="GeneID:144125154"
ORIGIN      
        1 mggsmttfdt dadkwqqhvm riedelalel lrafrvspat fryvveslqs vlqrqvtnmr
       61 kpitvekrva vglyrlcssa edrkiahllg igrstvntvf refcqavvnn lestwldmpr
      121 qmaehmrecy avtgfpqaig aldgchfais ppkkdaadyc nykgwhsiil laivdhqyqf
      181 lylnvgspgr chdahvygrs klkrivdsdr fnppvsvieg tcctnysv
//
LOCUS       XP_077514571             222 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125203 [Amblyomma americanum].
ACCESSION   XP_077514571
VERSION     XP_077514571.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658445.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 40% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..222
                     /product="uncharacterized protein LOC144125203"
                     /calculated_mol_wt=24619
     CDS             1..222
                     /gene="LOC144125203"
                     /coded_by="XM_077658445.1:1..669"
                     /db_xref="GeneID:144125203"
ORIGIN      
        1 medsctassa qnrkpkahwt deerayfsks gkitsakrnl qvyvamaecl raqeiarflg
       61 rlpindrslm detvddeyvs vemlidsmqq eppspaddes smtqaavsep pspppyesrc
      121 qpsalpspss plpaapstdh rlqtappnse aaehhhqtpa pnshhqtppp nshqtakpsr
      181 hatqksllqd lvntncelca dltrsrreem elkrmemeil qe
//
LOCUS       XP_077514807             242 aa            linear   INV 25-NOV-2025
DEFINITION  inactive selenide, water dikinase-like protein [Amblyomma
            americanum].
ACCESSION   XP_077514807
VERSION     XP_077514807.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658681.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..242
                     /product="inactive selenide, water dikinase-like protein"
                     /calculated_mol_wt=26106
     Region          42..241
                     /region_name="PurM-like"
                     /note="AIR (aminoimidazole ribonucleotide) synthase
                     related protein. This family includes Hydrogen
                     expression/formation protein HypE, AIR synthases, FGAM
                     (formylglycinamidine ribonucleotide) synthase and
                     Selenophosphate synthetase (SelD). The N-terminal
                     domain...; cl10019"
                     /db_xref="CDD:471963"
     Site            order(67..70,72,96,107,110,113,134..135,139)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:100027"
     CDS             1..242
                     /gene="LOC144125343"
                     /coded_by="XM_077658681.1:1..729"
                     /db_xref="GeneID:144125343"
ORIGIN      
        1 mharyngrnh ptifiisatl tpvecksavl vlfamvidie shdaqpvpgv digmdssvvp
       61 lgdfgyslaq tidyfyssve dtyiagkiac anvlsdlyal gvtrchntlv vmaastklte
      121 qqrdivvpll ikgikvgsap tavkpkmpdn aqhgdvlvlt kplgtqvavn vhqwrekper
      181 raqlgkagis eedvrrvyvr avdcmarlnl ngawlmhkhg chgatdvtgf gllghaqnlv
      241 qv
//
LOCUS       XP_077514926             438 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125406 [Amblyomma americanum].
ACCESSION   XP_077514926
VERSION     XP_077514926.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658800.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 80% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..438
                     /product="uncharacterized protein LOC144125406"
                     /calculated_mol_wt=49684
     Region          <4..>105
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(4..9,64)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238185"
     Site            65
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     Region          <70..330
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..438
                     /gene="LOC144125406"
                     /coded_by="XM_077658800.1:1..1317"
                     /db_xref="GeneID:144125406"
ORIGIN      
        1 maldikgafd nvsheavmer inntrcgrri hnyvraflsn rtvtvglgdl rsetfntpnk
       61 gtpqgsvisp vlfnmamigl arklskieee lrtkarilvp pnygrtmfgv ldetgtleyg
      121 qvfvqysndm lrykvndpat ilegdvivtk npcmhpgdir kleavnvpql hhvrdcivfp
      181 qkgerphpde magsvldgde ysvlwyedli fnnncnpmhy ysdppkdrka pigvqdmvdf
      241 ffqyikgvki glisnahlvw vdildssins hrcrelackc avnldfakcg dlkglqnsek
      301 ppmypdfmen ldtkntycsr kvlgqlyrnc kkvelstecl evvedslpdp rlllegreqf
      361 lkeatsaykr yaekirallk scriesesea lsgavsklsk ymkendptdm amvlesqveh
      421 vvhrtreeff sdqldeah
//
LOCUS       XP_077515056             112 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125483 [Amblyomma americanum].
ACCESSION   XP_077515056
VERSION     XP_077515056.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658930.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..112
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..112
                     /product="uncharacterized protein LOC144125483"
                     /calculated_mol_wt=13210
     CDS             1..112
                     /gene="LOC144125483"
                     /coded_by="XM_077658930.1:1..339"
                     /db_xref="GeneID:144125483"
ORIGIN      
        1 msgnlaylrr apegcpstel iriyinrede elfeilrrye dilteimmdw sgiqdiqcdl
       61 ktdrmdewfl qlmvtgsrwa lerlkeivvy psfrevllla ferkknayag tl
//
LOCUS       XP_077515298             517 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125606 [Amblyomma americanum].
ACCESSION   XP_077515298
VERSION     XP_077515298.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659172.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..517
                     /product="uncharacterized protein LOC144125606"
                     /calculated_mol_wt=55465
     Region          348..382
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(353,361,372..373)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(365,368,372,378..379)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            375..379
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          387..421
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(392,400,411..412)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(404,407,411,417..418)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            414..418
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          426..458
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(432,440,451..452)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(444,447,451,457..458)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            454..458
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          469..503
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(474,482,493..494)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(486,489,493,499..500)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            496..500
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     CDS             1..517
                     /gene="LOC144125606"
                     /coded_by="XM_077659172.1:1..1554"
                     /db_xref="GeneID:144125606"
ORIGIN      
        1 maqaaltpge ekagrevswr rlprqvgraa qpsvaaapaa ttdrdcsgat ssaavdthpd
       61 lkmkwsvqsa etltllqayv vlfvcrgvgv tipmrppgil psslalgsgc rsslecllfv
      121 anshcnwhkg avcdcqpyhv rqndtaclkt sllsygcald eqcslrvahs rcgsdglcqc
      181 rsgylplrld kclppakldd yclseqqcrl adpfsyckyi ipgvygkckc pngylltran
      241 eclpylesac rtdedcsrvt rhsrcgrkgd tsvcvcargf rmsrskmrce qdssgtsane
      301 valqaaslgq pcnataecrl rdkhsacvrg lcdclapttl csarstgcln dtfqcrsgec
      361 lswyfvcdgl kncadgsdes scvphrcpaa afqcddgtck sraavcngrw ecpdgsdear
      421 cyrgvkcdra afrcrsgqcv pqyalcnavv dcadgsdede qacvhgqrcp etsfrcanga
      481 crssailcsg vdgcgdnsde agcrvcqcar vqeadrs
//
LOCUS       XP_077515418             707 aa            linear   INV 25-NOV-2025
DEFINITION  atrial natriuretic peptide-converting enzyme-like [Amblyomma
            americanum].
ACCESSION   XP_077515418
VERSION     XP_077515418.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659292.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..707
                     /product="atrial natriuretic peptide-converting
                     enzyme-like"
                     /calculated_mol_wt=76729
     Region          220..336
                     /region_name="CRD_FZ"
                     /note="CRD_domain cysteine-rich domain, also known as Fz
                     (frizzled) domain; cd07066"
                     /db_xref="CDD:143549"
     Site            order(228,230,232,235..237)
                     /site_type="other"
                     /note="putative Wnt binding site [polypeptide binding]"
                     /db_xref="CDD:143549"
     Region          <348..366
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(349,352,356,362..363)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            359..363
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          369..403
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(374,382,393..394)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(386,389,393,399..400)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            396..400
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          512..680
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cl21584"
                     /db_xref="CDD:473915"
     Site            order(548,591,661)
                     /site_type="active"
                     /db_xref="CDD:238113"
     CDS             1..707
                     /gene="LOC144125662"
                     /coded_by="XM_077659292.1:1..2124"
                     /db_xref="GeneID:144125662"
ORIGIN      
        1 mpcdsgvsrh aqlrplqrgg hritftqrra gctlagclca slaalcltlg avagvyygvr
       61 flegqrtaer vyrgaftvle gdkfseslsn sladdfvrka dayrakldrl fnssaafrgt
      121 eviafqrred reltvhfnvh fspygpvdag elylamadel lrgrlgvfqg lkvdpeslsl
      181 qerrataslp eswtprrryp gylaglstsr pavgrqaelr cepltldhcr aslpynstay
      241 pnavghatar dvyrdlvayr qladsechpl aaelvcrllq pqcddaerrv vlpcrefcqe
      301 fwsscqqqmp laiaghidcg rlpeysaags chtkpgcane lkargkeshi cdgvvdcpdf
      361 sdetscdncg pgllhcgdrq cvdiaqrcda rldcdngade qnclslpelg gylrayqrgh
      421 frkvcvdglp egrrrdsllk qladtacaal nlgppsrvel hhdgnssdsy lqlldppglr
      481 fsspgacrsd lvvyllcspp ecgqssalpt tqevrhgdwp whvmllqagr hvcdgtlvad
      541 rwvltagacl pqgrdpsgew qarlgsvrke saspfdvraa iaavlrsadg vlallrldaa
      601 vpqsravrpa clprtpppqa grcatlaygv kgdqleqvnl easecgpavl cgrqascvhe
      661 slagrqlmcw hgdrwqlagv aaepcsggaq rfhglgphla waeaals
//
LOCUS       XP_077515657             465 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125790 [Amblyomma americanum].
ACCESSION   XP_077515657
VERSION     XP_077515657.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659531.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..465
                     /product="uncharacterized protein LOC144125790"
                     /calculated_mol_wt=51728
     Region          68..>148
                     /region_name="Ty3_capsid"
                     /note="Ty3 transposon capsid-like protein; pfam19259"
                     /db_xref="CDD:437091"
     Region          115..200
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     Region          <283..319
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     Region          347..437
                     /region_name="Asp_protease_2"
                     /note="Aspartyl protease; pfam13650"
                     /db_xref="CDD:433378"
     CDS             1..465
                     /gene="LOC144125790"
                     /coded_by="XM_077659531.1:1..1398"
                     /db_xref="GeneID:144125790"
ORIGIN      
        1 msetrsgata gttdgqdggk vsdsisvsdf lsamrekdrl lasllemlal passgapgva
       61 aatttyqvmp dlsknisdfd gsgdaasake wlgnlqttat lhrwpepfkl etakchlvgp
      121 akdwlrsrke vstswenfdy hfrrtfcgqt raaerwrkmq grvqqrnest ttyfhskvrl
      181 creanldfcd treqvltglr srqlctmllg rthdddddll hdimeferid rereqrfggt
      241 rekrhfpaat ervaerppar kstydrfgkd rrppltnerg qfkcynsgrf ghisrdcpee
      301 rhemkclrcg ksghtqrhcs msqsnetntv tdteqkiaag allkqpmlng tttlvgmidk
      361 gssgcllras aaarcgarlt kdatalygfg sqgtpsarai gkcytdleid gvkgenvpfl
      421 avpddtltvd llvgrtytel pyvayarpgg clrfwhksvc pfshl
//
LOCUS       XP_077515897             363 aa            linear   INV 25-NOV-2025
DEFINITION  transmembrane protease serine 2-like [Amblyomma americanum].
ACCESSION   XP_077515897
VERSION     XP_077515897.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659771.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 24% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..363
                     /product="transmembrane protease serine 2-like"
                     /calculated_mol_wt=38672
     Region          <3..22
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(5,8,12,18..19)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            15..19
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          25..59
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(30,38,49..50)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(42,45,49,55..56)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            52..56
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          168..336
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cl21584"
                     /db_xref="CDD:473915"
     CDS             1..363
                     /gene="LOC144125918"
                     /coded_by="XM_077659771.1:1..1092"
                     /db_xref="GeneID:144125918"
ORIGIN      
        1 makshicdgv vdcpdfsdet scdncgpgll hcgdrqcvdi aqrcdarldc dncadeqncl
       61 slpelggylr ayqrghfrkv cvdglpegrr rdsllkqlad tacaalnlgp psrvelhhdg
      121 nssdsylqll dppglrfssp gacrsdlvvy llcsppecgq ssalpttqev rhgdwpwhvm
      181 llqagrhvcd gtlvadrwvl tagaclpqgr dpsgewqarl gsvrkesasp fdvraaiaav
      241 lrsadgvlal lrldaavpqs ravrpaclpr tpppqagrca tlaygvkgdq seqvnlease
      301 cgpavlcgrq ascvhealag rqlmcwhgdr wqlagvaaep csggaqrfhg lgphlawaea
      361 als
//
LOCUS       XP_077516134             925 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126062 [Amblyomma americanum].
ACCESSION   XP_077516134
VERSION     XP_077516134.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660008.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..925
                     /product="uncharacterized protein LOC144126062"
                     /calculated_mol_wt=102483
     Region          257..344
                     /region_name="RP_Saci_like"
                     /note="retropepsin family; cd06094"
                     /db_xref="CDD:133158"
     Site            order(271,273,275,294..296,333)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133158"
     Site            271..273
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133158"
     Site            271
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133158"
     Region          423..598
                     /region_name="RT_LTR"
                     /note="Reverse transcriptases (RTs) from retrotransposons
                     and retroviruses which have long terminal repeats (LTRs)
                     in their DNA copies but not in their RNA template. RT
                     catalyzes DNA replication from an RNA template, and is
                     responsible for the replication of...; cd01647"
                     /db_xref="CDD:238825"
     Site            order(487..492,521..522,547,549..550,594..595)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238825"
     Site            order(487..492,521,549)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238825"
     Site            522
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238825"
     Region          665..>716
                     /region_name="RT_RNaseH_2"
                     /note="RNase H-like domain found in reverse transcriptase;
                     pfam17919"
                     /db_xref="CDD:465567"
     CDS             1..925
                     /gene="LOC144126062"
                     /coded_by="XM_077660008.1:1..2778"
                     /db_xref="GeneID:144126062"
ORIGIN      
        1 mnqpsdagsp tvavldvklp pfwtgdpelw fvqvesqfaa rritadstky hhvvgslppa
       61 tasevrdlll tppaenayqt lketlirrvt pteperlqql lreaelgdrr psqllrhmqq
      121 laggttasdg rlvrelflqr lptsvriglt asgetdlakm aeladklmav avptvasvha
      181 dapsanslqe mreeisrlad tvaalhssgn qrprqrtasq pqqrrvcwyh rkfgnaarns
      241 lrvtsapalp dsrpsvfflt drergarflv dtgaevsvvp aspadrkrpc aaplqavnnt
      301 tiptygqrsl sldlglkrtf rwifvvadvk failgadflr nfgllvdvrn rrlqdplsha
      361 evrgkpsrhp plsptlvapp gatrftgilg efpeltrppq asaavkhnlq aarqefdhml
      421 elgiirpsas pwasplhmvp kstpgdwrpc gdyralnrat vpdrypvphi hdvtcslhgv
      481 aifskidlvr ayhqipvape dvsktaittp fglfeflrmp fglrnsgqtf qrfingvlhg
      541 ldfchayldd lliastssde heahlrsvfq rlaehgilvn tdkcelgaek ptflghvvss
      601 sdiqphpdkv kavekfprpt tkrqlreflg lvnyyrrfvp rcvavlhplh lllsthegaa
      661 rellwtddae aafqeikrhl adatllaypq pgvpqcvtvd asdaavgavl qqlnkglkaa
      721 lmasehdswv ealpivllgt rtaceadigc saaelvygtt lrlpgdffas dqqrarisas
      781 dyasrlrdim nklratpprq paerrthvsn elescshvfl rndavrrplq apydgpfkvl
      841 rrgrktftie mrgrrevvsl drlkpaymet qsmtptsips pgthtrvprp raavstreen
      901 astpdtrtyh trsgrrlnap srlnl
//
LOCUS       XP_077516377             683 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier organic anion transporter family member 4A1-like
            [Amblyomma americanum].
ACCESSION   XP_077516377
VERSION     XP_077516377.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660251.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..683
                     /product="solute carrier organic anion transporter family
                     member 4A1-like"
                     /calculated_mol_wt=75144
     Region          75..634
                     /region_name="OATP"
                     /note="Organic Anion Transporter Polypeptide (OATP)
                     family; pfam03137"
                     /db_xref="CDD:460821"
     CDS             1..683
                     /gene="LOC144126199"
                     /coded_by="XM_077660251.1:1..2052"
                     /db_xref="GeneID:144126199"
ORIGIN      
        1 meldqqqrvw asvlprcgep rvveeeeegr skqhcgtsda mlrsrqareg seadthcgww
       61 gwhpaplqrf asaewvlvcf cvagfvqgmv isgfinvslp tlerrfhlrs veaglivsmy
      121 nvgslmlmtp vtffgsemhk primangvff mgvgsflftl phfvapqyky sqevkdlcpf
      181 eedkatiinp egsralrnyr fvfmvanlvh gcstvpfytl aiaylddnlt arksswyigi
      241 yytaaiigpa vgfilggmll nvytditvdp rslglspssn vwigawwigf litgcsailv
      301 svpisgfpkt lpgtaemlaq krvevdtral eaehdavrlr lsdmpkavyh llrnntfvfi
      361 slaatvetmi gsgysnfatk lfenqfgmta asaalllgmi aipsaslgcf lggyvvskld
      421 lscaniirmc ivvsvvtwfv ffmllvscpn lhyegiditk klvltpkcts ecgcpltfnp
      481 icgrdfkmym spclagcrna aqneeavtvy gncdciptes sdnsstefvv aaervrctqe
      541 csaylfytlg tficlfstfy isapgisvti rcvtakqksf algiqwamir llgtipapli
      601 fgktvdmace iwssvfdgla nesgncvlyn naslsrnmst llillktmsv vlftcaslsy
      661 rpaaedmrst tqemsphene kma
//
LOCUS       XP_077516493             861 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126257 [Amblyomma americanum].
ACCESSION   XP_077516493
VERSION     XP_077516493.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660367.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 24% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..861
                     /product="uncharacterized protein LOC144126257"
                     /calculated_mol_wt=96455
     Region          <433..>605
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     CDS             1..861
                     /gene="LOC144126257"
                     /coded_by="XM_077660367.1:24..2609"
                     /db_xref="GeneID:144126257"
ORIGIN      
        1 mrfafsllcf vsaaqgsvhq rrgegdmepa syqgrilpad lssqghaddr ttgyshrrrr
       61 gridaghghv grlpdnegva vttrapdgpr rrasdeerat pyatkdpklv trrlgrivfc
      121 lvvacvalla lllclailvl wlrsryrtrg kalyntlsvt dpdvptpaas skshqwrlsd
      181 dslsseatva atdrhsraar qslsepivws mkttdmrdgs phrhssesgl ssatpseigf
      241 qkattpayad vdpggarkrr kslrvkstda mlpqrsysir rtdvaerqpk rdrrnkrydt
      301 krtrrkrrss vdhrpseqgs vvsgpaihps vsftdkandl sedyqqysys ddvrldvrlg
      361 plptfirqsv tktvrrwrth asdarstlsa erraeagrpf rrpwsarssf esltisepqe
      421 egchiktdat pwtqllalck qskstdflqi iqegceatrl hwdegggdvf rvlagsaare
      481 rlvrvvqlrp qdvhwhagrl rlarelrglr ngvhnrtsaf sldvrtslvr dtyprilwda
      541 lrhdqdirsp keleqpkhfl vtemqpgwrp ltsfqlsdps qaasvlqqac walavaeaal
      601 qfehrlpgan avllrptrsp rvefmlhgrl erlasagvki rlqglqmarm sidrtpeytd
      661 lrrfhefvtg eeatavfrkm arllrepvel npgeagwktn esglfvtteg vatsstsesv
      721 veeqpvpaae iedggeqtrv slppesdsva tievvgteek aslalqsega lgqspedgee
      781 srrfteksey cmpdcrssdr eeersmskre kapahlpltr apsvknrnlf gvnpraarrp
      841 vpmrkvmkil pvrkvkvqck e
//
LOCUS       XP_077516579             304 aa            linear   INV 25-NOV-2025
DEFINITION  chitotriosidase-1-like [Amblyomma americanum].
ACCESSION   XP_077516579
VERSION     XP_077516579.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660453.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 41% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..304
                     /product="chitotriosidase-1-like"
                     /calculated_mol_wt=33596
     Region          17..>286
                     /region_name="GH18_chitinase-like"
                     /note="The GH18 (glycosyl hydrolase, family 18) type II
                     chitinases hydrolyze chitin, an abundant polymer of
                     beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a
                     major component of the cell wall of fungi and the
                     exoskeleton of arthropods. Chitinases have...; cl10447"
                     /db_xref="CDD:471972"
     CDS             1..304
                     /gene="LOC144126768"
                     /coded_by="XM_077660453.1:248..1162"
                     /db_xref="GeneID:144126768"
ORIGIN      
        1 mganvvvlgm sggrtavvcy rnspsllraa pkdydlgklc ahccthlfyt garvdpnglr
       61 lqfqeddtgl ksvrdmdalk tshpqlrlvl svqhvgsgpl nltegtdaas rralladsma
      121 hwlgefnfeg veldwdrlpv dlnpvyvglv ramridtkki llltlvsihm kggpshnvea
      181 rhsmsclliv hayskedtlk tavppiifke dllaaliqrs mhdaqveace tflgelrfrq
      241 rvvlalfgya yrlrnsgrye lrasvrgpap pgpftkergl lvyfevlmsc llyaatnasr
      301 rhgh
//
LOCUS       XP_077516582             390 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126926 [Amblyomma americanum].
ACCESSION   XP_077516582
VERSION     XP_077516582.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660456.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..390
                     /product="uncharacterized protein LOC144126926"
                     /calculated_mol_wt=43401
     Region          <180..240
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     Region          259..>364
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..390
                     /gene="LOC144126926"
                     /coded_by="XM_077660456.1:1..1173"
                     /db_xref="GeneID:144126926"
ORIGIN      
        1 mhdlpvnwkh elsefwsggv llafpdfprf hvvhseheeq tarqvspfiv trtltsaige
       61 gykiktlang dlllevlynh qqeklsevks fgdipviist hrslntvrgv isdddlkyvt
      121 deelleglke qnvtnvyrik irrdneeipt kqialtfafs ilpdsveigy iklqlrqyip
      181 nprrcfkcqr yghssqscrg qltcakcsfh ehdaencave phlcvncegs hpaysracpv
      241 wkqekeivti kvkenitfre arkrvasihk ptfsdvvqrg tapqpllapt qatrsepvag
      301 ppvpkvaaas aappptkqda rasrsagpkv pphterpnmk tnvrlarsss vsedamdtmd
      361 tsqsspkast skgrrgsidr kkrkapnkga
//
LOCUS       XP_077516594             175 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127244 [Amblyomma americanum].
ACCESSION   XP_077516594
VERSION     XP_077516594.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660468.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..175
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..175
                     /product="uncharacterized protein LOC144127244"
                     /calculated_mol_wt=18956
     CDS             1..175
                     /gene="LOC144127244"
                     /coded_by="XM_077660468.1:1..528"
                     /db_xref="GeneID:144127244"
ORIGIN      
        1 mtrqgiarvg rpaagsawsa gilfvsvggd arvsgavqdl vfllrehvas vvlvfkilql
       61 hyddphkvrf edrrrrhlnr rrdshdealv grarsqsras fpgcfwrada vlicrgpipr
      121 getpghrpqc afvplqwqvq gksghdnsat ticplgslsa atsfpesvqr aglgg
//
LOCUS       XP_077516734             429 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127628 [Amblyomma americanum].
ACCESSION   XP_077516734
VERSION     XP_077516734.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660608.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 20% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..429
                     /product="uncharacterized protein LOC144127628"
                     /calculated_mol_wt=46649
     CDS             1..429
                     /gene="LOC144127628"
                     /coded_by="XM_077660608.1:1..1290"
                     /db_xref="GeneID:144127628"
ORIGIN      
        1 maslgdtrsn skfvlrdatl yrrhwlknrk keasissyfl llsagrcstp fttspqegtl
       61 anpaqemcsl rkrvkeyqhk nkpwkadarr rhfvflpttl vqrdnharrr eevplcaqgt
      121 glrdghvagg dtspspldng gllradcsrv ridtapvtns ditqasareq stldrleptp
      181 atarktalfa dasgasagsd angtasyaii dlsvanvlld avqcktcrrc vrlitsdqsy
      241 glavklivlc dncgeiaaew nsrrvdgekk cnpfelnila sramlstgna qtamndifsv
      301 mrlsqrgmhn ktfqwhlknt lqpaatraav aamaqatqav akvyddlcfg hrgniavvgy
      361 tgipssspap aatvtytgal atrnverrhp rdsahfrpst dvtsrqraaf cgegstpkld
      421 gndllrgrq
//
LOCUS       XP_077516769             374 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127717 [Amblyomma americanum].
ACCESSION   XP_077516769
VERSION     XP_077516769.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660643.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..374
                     /product="uncharacterized protein LOC144127717"
                     /calculated_mol_wt=40336
     Region          <155..226
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     Region          <285..321
                     /region_name="zf-RVT"
                     /note="zinc-binding in reverse transcriptase; pfam13966"
                     /db_xref="CDD:433612"
     CDS             1..374
                     /gene="LOC144127717"
                     /coded_by="XM_077660643.1:1..1125"
                     /db_xref="GeneID:144127717"
ORIGIN      
        1 mrtfkdyqnl aettteflwn krrqslpkap mkqteyhtpl cktfdsrvva fdafchentg
       61 qanvqavhgk dfcvstlagt vgkgaevdas sdgcvlsffd gtdgsvlpat gqaaaacvap
      121 algaalscrl rvpanstaae laglhlaadl llllpgqqpa viltdsraal qllrqhqprq
      181 htvanltarl maiqdagrpv slqwlpshvg itgneaadql agsahtngsp vsskvtqldf
      241 arpalrqavr alhpdprvas gvrfmrvpdc lprrertlll rlrtgcswtp arlhrhgrap
      301 spacsfcgad etlghllcac pnleaarrdm tagyrnlglp sysdqdllhp ersqteafrl
      361 lleflqstrl atrl
//
LOCUS       XP_077516866             289 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127791 [Amblyomma americanum].
ACCESSION   XP_077516866
VERSION     XP_077516866.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660740.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..289
                     /product="uncharacterized protein LOC144127791"
                     /calculated_mol_wt=33076
     Region          53..>180
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     Region          <176..>249
                     /region_name="V_Alix_like"
                     /note="Protein-interacting V-domain of mammalian Alix and
                     related domains; cl14654"
                     /db_xref="CDD:353824"
     CDS             1..289
                     /gene="LOC144127791"
                     /coded_by="XM_077660740.1:1..870"
                     /db_xref="GeneID:144127791"
ORIGIN      
        1 mvhrnevnnr ikvvenashp evaprqgtvr vcrtgrcley arrlrntlnt slnpcesftr
       61 fvcdglrrrn rlsvreeafl gvvrklssll lsvpvpsrgq tslqraaafy rscddvlqgr
      121 adelrkvkka lldagigwpr lpkdsnrsdl lstwfhasir lhwspllhvm lqqdlnvttv
      181 vleplsdfer ilrkeksleg sgheakiyfe elrreflanc ehdngkkdnt tvtyaitkrl
      241 veshvtplrh afgsmhlfds gldtafmfrk msgltkqcwl qeldyyvgp
//
LOCUS       XP_077517077             320 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127918, partial [Amblyomma
            americanum].
ACCESSION   XP_077517077
VERSION     XP_077517077.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660951.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         <1..320
                     /product="uncharacterized protein LOC144127918"
     Region          74..125
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          155..314
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..320
                     /gene="LOC144127918"
                     /coded_by="XM_077660951.1:<1..963"
                     /db_xref="GeneID:144127918"
ORIGIN      
        1 kenkkqerdi dvlkkalhys keraaeaeqn vqqgrfsanc iteqcvsvyt glpskalflf
       61 fvslfknval yipnrctvad avlvvlmklr lallnidish rfglsettva rimvknipii
      121 aselkrfiiw psrkdacrtm pmmvrrkypt cmaiidctev riqkplgysa rsktyshyks
      181 sntikfmvsi tpcgaisfvt kcwggrasdk eiilksgfld klqegdivla drgfliredv
      241 ahrgarlevp altrgkrqls akevedvrhi srvrihvera igrikvfhil rdrlsitmlr
      301 hatdivvica aitnmkprla
//
LOCUS       XP_077517266             395 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128059 [Amblyomma americanum].
ACCESSION   XP_077517266
VERSION     XP_077517266.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661140.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 28% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..395
                     /product="uncharacterized protein LOC144128059"
                     /calculated_mol_wt=44388
     Region          133..>169
                     /region_name="FH_FOX"
                     /note="Forkhead (FH) domain found in Forkhead box (FOX)
                     family of transcription factors and similar proteins;
                     cl00061"
                     /db_xref="CDD:469596"
     CDS             1..395
                     /gene="LOC144128059"
                     /coded_by="XM_077661140.1:1..1188"
                     /db_xref="GeneID:144128059"
ORIGIN      
        1 mnpnrgiagr grtassepak rsrrtrtmss hgdtctmpql hpgplaellp agtivrrppg
       61 rlhdygryrd cllhghlaye nlahlarqaf pphphhvqpr rhvhhapats gspgraapcw
      121 hhrpqasgqg klvqlvaqai hespikalrv rhvyealkkr ypyfmfmdkk elttwegses
      181 rpssrlssss qsllvhtplp sfigspgrql ghmepqlhpl vewelkterf atppspsiyl
      241 nmeqerkpkp vyswsrgcyd lyghqsqgys devstaadtq llneplpslp ylcsprlthd
      301 tpveptsrsy ghpsphqepq lpltleqhsr pelsfarqqi qpeqeammpr qqflprpels
      361 nsregtlphp elplppqfpw ppqgpslprg ptlpp
//
LOCUS       XP_077517371             395 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128125 [Amblyomma americanum].
ACCESSION   XP_077517371
VERSION     XP_077517371.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661245.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 28% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..395
                     /product="uncharacterized protein LOC144128125"
                     /calculated_mol_wt=44360
     Region          133..>169
                     /region_name="FH_FOX"
                     /note="Forkhead (FH) domain found in Forkhead box (FOX)
                     family of transcription factors and similar proteins;
                     cl00061"
                     /db_xref="CDD:469596"
     CDS             1..395
                     /gene="LOC144128125"
                     /coded_by="XM_077661245.1:1..1188"
                     /db_xref="GeneID:144128125"
ORIGIN      
        1 mnpnrgiagr grtassepak rsrrtrtmss hgdtctmpql hpgplaellp agtivrrppg
       61 rlhdygryrd cllhghlaye nlahlarqaf pphphhvqpr rhvhhapats gspgraapcw
      121 hhrpqasgqg klvqlvaqai hespikalrv rhvyealkkr ypyfmfmdkk elttwegses
      181 rpssrlssss qsllvhtplp sfigspgrql ghmepqlhpl vewelkterf atppspsiyl
      241 nmeqerkpkp vyswsrgcyd lyghqsqgys devstaadtq llneplpslp ylcsprlthd
      301 tpveptsrsy ghpsphqepq lpltleqhsr pelsfarqqi qpeqeammpq qqflprpels
      361 nsregtlphp elplppqfpw ppqgpslprg ptlpp
//
LOCUS       XP_077517611             174 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128218 [Amblyomma americanum].
ACCESSION   XP_077517611
VERSION     XP_077517611.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661485.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..174
                     /product="uncharacterized protein LOC144128218"
                     /calculated_mol_wt=19442
     CDS             1..174
                     /gene="LOC144128218"
                     /coded_by="XM_077661485.1:143..667"
                     /db_xref="GeneID:144128218"
ORIGIN      
        1 mvmvcvdavs aqnaaefpmq hflyrepmss alagfalkkk cyreptssqc tceifltvpd
       61 garypagyar scsiwanqrm lscgapmrrv lrgrrpptra qpldeqrell aqqaptkhrg
      121 lalqwrhlpp keglyvtgeq lppivgpkay rcfyhaeids rhlaleragg lawt
//
LOCUS       XP_077517721             341 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128270 [Amblyomma americanum].
ACCESSION   XP_077517721
VERSION     XP_077517721.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661595.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..341
                     /product="uncharacterized protein LOC144128270"
                     /calculated_mol_wt=38078
     Region          159..242
                     /region_name="retropepsin_like_LTR_1"
                     /note="Retropepsins_like_LTR; pepsin-like aspartate
                     protease from retrotransposons with long terminal repeats;
                     cd05481"
                     /db_xref="CDD:133148"
     Site            167..169
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133148"
     Site            167
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133148"
     CDS             1..341
                     /gene="LOC144128270"
                     /coded_by="XM_077661595.1:1..1026"
                     /db_xref="GeneID:144128270"
ORIGIN      
        1 mtelpppepl rfganvadnw trfkqrvely fiatestepg rqrsnaqkaa illhlagqea
       61 idvyntfdlt aedkkdydkl vqafeayclp qsnetferyv frnrtqaevc ckaraadvne
      121 vaerpddddd ftvlhvstac kkasaadard wevatrisgr evrlkvdtga qanllprglf
      181 ealgtkqqpe paravlrsyg gneivhmgkv clpvrigadt rlveffvvkk grqailglqa
      241 ceqfglvnri ravkkdeals ecvrrqypkl fmgigklkre ysmtlqdgar pvaeparrvp
      301 hairsrlkee ldrleaagli akvrepsdwp fpssmvatgg l
//
LOCUS       XP_077517835             222 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128336 [Amblyomma americanum].
ACCESSION   XP_077517835
VERSION     XP_077517835.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661709.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..222
                     /product="uncharacterized protein LOC144128336"
                     /calculated_mol_wt=23707
     Region          <125..>158
                     /region_name="tetraspanin_LEL"
                     /note="Tetraspanin, extracellular domain or large
                     extracellular loop (LEL). Tetraspanins are trans-membrane
                     proteins with 4 trans-membrane segments. Both the N- and
                     C-termini lie on the intracellular side of the membrane.
                     This alignment model spans the...; cl02781"
                     /db_xref="CDD:351888"
     CDS             1..222
                     /gene="LOC144128336"
                     /coded_by="XM_077661709.1:1..669"
                     /db_xref="GeneID:144128336"
ORIGIN      
        1 mqgplkkavg afaavllmsa vamiavgshw hgrlspyskf ytgrlgtpii livmgvlsfp
       61 lalllvpglr rdnyralyvf agilacmvac eigcavasfe yrhvigaavr svvlrdldgc
      121 qgtgldfaqr tlaccggpng shdyrarglp ippsccphgc qrygahrasc dyrleglfnr
      181 amidvgatai allcirfmgv llvcwqaqrv sadnalvytv nq
//
LOCUS       XP_077517951             167 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128409 [Amblyomma americanum].
ACCESSION   XP_077517951
VERSION     XP_077517951.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661825.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..167
                     /product="uncharacterized protein LOC144128409"
                     /calculated_mol_wt=18786
     CDS             1..167
                     /gene="LOC144128409"
                     /coded_by="XM_077661825.1:474..977"
                     /db_xref="GeneID:144128409"
ORIGIN      
        1 mrphryisks nfeddgqrks prkdhqfypa ppsdfpsstl sdcialakpm ltapettahg
       61 atqdhgedma dllipqqaya tpnqgqstaa paiperefqr qvmrqlqvir liveqnrdtl
      121 enmlgsrkee eaaltlvcqp fddlqewrvf dntipdvkdq lvsakhc
//
LOCUS       XP_077518069             705 aa            linear   INV 25-NOV-2025
DEFINITION  neprilysin-1-like [Amblyomma americanum].
ACCESSION   XP_077518069
VERSION     XP_077518069.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661943.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..705
                     /product="neprilysin-1-like"
                     /calculated_mol_wt=78567
     Region          97..702
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..705
                     /gene="LOC144128476"
                     /coded_by="XM_077661943.1:1..2118"
                     /db_xref="GeneID:144128476"
ORIGIN      
        1 maernhtdaf aaalstfpka ttrlqlatqv getnagrgat avkrcvalca aslcgfailv
       61 wvcvrlaaet kasrcgtpec dryaailhns sasavgpced fydyvcarws gaqdsvqhvl
      121 rtevqrdvft rllrmdvptk nqrawhkaak llqtcldvaq rsqspvepli rfmrhvgllw
      181 plpshvivld llvkmslywg ipvwgafqvd slnrdlrhrp llvfgetvef dqwlqrkham
      241 yhavqydqyl saylrafglp tnrtndtila iaaadalvdr rlipllgllg dsivtfaglp
      301 ggvnltdsln rlaywtkeky ttgdivrvhn lnllyeilnl atlapadvls lsfgwsvarq
      361 lgqyvsrdla mvqyfafeaa deslrtdcfd fvwsfmtism gtpyvaqkvt telheditgl
      421 vkqlqdnler iifessilsh dykenasfil rsmkkvlfwp egmrgerdvn rlfgdvpdlr
      481 mpvfldnwvl traalrnvtv siipqvyrfp slgaapvydv gnnalavpvg atsfpmysse
      541 lvpaanyggl artltaamag alywehhpwl rgatlslyrr rldclvsmfh tyqgparvga
      601 pgrygaadgv dqsavfvasw tlaplhaayr savagsrrtv lsgarnldae qlffvsfcls
      661 qcsnedsldn rllcnvplrn fpsfasafrc ardtymnpqr rclvw
//
LOCUS       XP_077518312             478 aa            linear   INV 25-NOV-2025
DEFINITION  palmitoyltransferase ZDHHC20-B-like [Amblyomma americanum].
ACCESSION   XP_077518312
VERSION     XP_077518312.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662186.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 23% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..478
                     /product="palmitoyltransferase ZDHHC20-B-like"
                     /calculated_mol_wt=53254
     Region          1..297
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; cl19890"
                     /db_xref="CDD:418707"
     Region          <394..478
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..478
                     /gene="LOC144128613"
                     /coded_by="XM_077662186.1:29..1465"
                     /db_xref="GeneID:144128613"
ORIGIN      
        1 matssggses arrrrsnhef vawlpvamvv glftwayyvy ifvfcgslvk esaqrfafst
       61 vfhlllllcl wsfvqttvta vppipgyfgl sesdqrllek caddeargef ldilaanrgv
      121 ltrgpsggvr fcercqqvkp nrahhcsqcr rcfpkmnhhc pwfnncvcfs tykfflltif
      181 hvvalcvfgl tsathhvaga wssnastyvt lnctflyafg vmlvlvlgsf lylhftmvcn
      241 nvttledlrp hlfkddrdsf digfrdniae vfghrkalwf lpvftslgdg arfptrlhpd
      301 ryvygppvsv vveerrpsvp vpplpvadev pelpvltscr rpvpstegpl ggltltpyva
      361 pprgslsflp pttitvvppt eaaasvrlse skkdlppivv aseftsvesf aalssrlssv
      421 pgmfrrktfl hwytgegmne yefkeaesnl ndlvseyqky qeataedege feeaqaea
//
LOCUS       XP_077518559             171 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128742 [Amblyomma americanum].
ACCESSION   XP_077518559
VERSION     XP_077518559.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662433.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 47% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..171
                     /product="uncharacterized protein LOC144128742"
                     /calculated_mol_wt=19586
     Region          51..71
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(56,58,60,62..63,66..67,70,84,86,90..91,94..95,98,
                     112,114,116,118..119,122..123,126)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          <73..151
                     /region_name="SFP1"
                     /note="Putative transcriptional repressor regulating G2/M
                     transition [Transcription / Cell division and chromosome
                     partitioning]; COG5189"
                     /db_xref="CDD:227516"
     Region          79..99
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          107..127
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          135..155
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          147..171
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     CDS             1..171
                     /gene="LOC144128742"
                     /coded_by="XM_077662433.1:1..516"
                     /db_xref="GeneID:144128742"
ORIGIN      
        1 mealptvdgk rsaapprifc dtcgalfpaq dllemhrstm hpprpqgrhr caycdfsrds
       61 kshvtrhert htgdrpfvce vcdkgftqrw nlsrhmlmhl gqkphqcpec gqrfsrgshv
      121 trherthtgd cplvcevcdk gftqrwnlsr hmlmhlgqkp hqcpecgqcf s
//
LOCUS       XP_077518803             775 aa            linear   INV 25-NOV-2025
DEFINITION  attractin-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077518803
VERSION     XP_077518803.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662677.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..775
                     /product="attractin-like protein 1"
                     /calculated_mol_wt=85302
     Region          <25..112
                     /region_name="NanM"
                     /note="N-acetylneuraminic acid mutarotase [Cell
                     wall/membrane/envelope biogenesis]; COG3055"
                     /db_xref="CDD:442289"
     Region          34..92
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          289..333
                     /region_name="PSI"
                     /note="Plexin repeat; pfam01437"
                     /db_xref="CDD:396154"
     Region          406..451
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(406,408,414,420,424,433)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          452..499
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(453,455,462,469,471,481)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     CDS             1..775
                     /gene="LOC144128867"
                     /coded_by="XM_077662677.1:1..2328"
                     /db_xref="GeneID:144128867"
ORIGIN      
        1 mfffaskgye lndqrytatt ksvetllaps ashrylhsav llhrmmlvfg dnthndtafs
       61 tgdkcfsadl laydiecdsw qtlsvpsssy lqlerfghsa vvynesmyif ggfngqmlts
      121 ivkftpgscr sfstpencit satgikcawn pdkkvcqsfh lfnatkgiht cprksaanlt
      181 vlcekqtsfp sclqnnygcv wcgtvcahkr cpkgskglad arenqtdveq cqqsessncd
      241 klhnchacht efhcgwqndh rcytfvrdsg nktekavlsd nyrvkcdnsc ssrttckaci
      301 lgscmwcisq qrcletnaya atfplaqcte wittkcsals cedfqtcsac qehercgwcd
      361 dgsntgkgqc legsasgpls pagstyrvng slcpapnwyf tdcpkcqcng hstcngssec
      421 lqscehrteg phcehciagy hgnpanggnc tpcfcnnhgt fchretgkcn cttkgiigpn
      481 cdrcdeqnhy vgspqeeggt cfynlsidfq ytfnlssedy yrninfmnvp ikvvndveft
      541 iscsgkayvn isigsggqpp sradgdaepa lldsrkcdni klpfydekhn fgsqnttffv
      601 yvfnfttpfv lqisfsqhra ldllqffitf sscflsllii aailwkikqk ydlyrrrqql
      661 fvemeqmasr pfagvtleld sepdgsskqf qdgmkqaqgh ptpvalepca sgkaavltli
      721 vrmptggqeh tatgqsglai asalvslgtl cksdtikedg ksdcwkpssh pgtci
//
LOCUS       XP_077518924             121 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128936 [Amblyomma americanum].
ACCESSION   XP_077518924
VERSION     XP_077518924.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662798.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..121
                     /product="uncharacterized protein LOC144128936"
                     /calculated_mol_wt=11885
     CDS             1..121
                     /gene="LOC144128936"
                     /coded_by="XM_077662798.1:921..1286"
                     /db_xref="GeneID:144128936"
ORIGIN      
        1 mdcsqspset sasvrragep aavsgepvsk gyvqsrssqs lllfqkdadg asptgstlnq
       61 acshasgfim vqsdhtyagp steasassle pgtagvaatl svsadlspga pfsssplcce
      121 g
//
LOCUS       XP_077519041             154 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129010 [Amblyomma americanum].
ACCESSION   XP_077519041
VERSION     XP_077519041.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662915.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 42% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..154
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..154
                     /product="uncharacterized protein LOC144129010"
                     /calculated_mol_wt=17121
     CDS             1..154
                     /gene="LOC144129010"
                     /coded_by="XM_077662915.1:1..465"
                     /db_xref="GeneID:144129010"
ORIGIN      
        1 mealptvdgk rsaapprifc dtcgalfpaq dllemhrstm hpprpqgrhr caycdfsrds
       61 kmkkgfrnmk ismargfgmr adpdtmflld nddfdpmnyk skrdirrisl statgfgkrl
      121 spgssdeqpa atwlaeevak gdlnhggaly qdtf
//
LOCUS       XP_077519152             259 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129088 [Amblyomma americanum].
ACCESSION   XP_077519152
VERSION     XP_077519152.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663026.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..259
                     /product="uncharacterized protein LOC144129088"
                     /calculated_mol_wt=27467
     Region          <139..197
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..259
                     /gene="LOC144129088"
                     /coded_by="XM_077663026.1:307..1086"
                     /db_xref="GeneID:144129088"
ORIGIN      
        1 mstafiptgq ptaekgnges eeeassssnp dgsrgnrarq grragaarga rpgreapaes
       61 gdepaggled slkgaaapgg srglparrpe grrgrlrggr iargqlhadi amsvgnedev
      121 aaptsslarr arqagqaarf pqgeknkivr qhwianlerk dfnrgsssrv csvhfidgrp
      181 tkdnpyptlh lgtptvhhng irklarhdvt plepdacsta eplshhssde qdkesasadh
      241 hlpinvgted lsdryskvv
//
LOCUS       XP_077519275             279 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129158 [Amblyomma americanum].
ACCESSION   XP_077519275
VERSION     XP_077519275.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663149.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 48% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..279
                     /product="uncharacterized protein LOC144129158"
                     /calculated_mol_wt=30498
     CDS             1..279
                     /gene="LOC144129158"
                     /coded_by="XM_077663149.1:1..840"
                     /db_xref="GeneID:144129158"
ORIGIN      
        1 matppkrprm svaqglllie lleqhpplar aaselspeyp aarrgelwer iaaalnregp
       61 avksaelwgt sggrlsgrag hvvqlvgpeq aspphslspp rghrasvgsv pgtsglqreh
      121 rsaaddtnms wagasettpl elleeeqqhq haeiastqqp talstlpsfa sppsppvpgr
      181 pvwrrrqrde evadllretr rtadltearn rrdaefqarl lehlneaaad rrqltatvad
      241 laaavrdtre qsvrlheqlv vavsflllml hyqihppph
//
LOCUS       XP_077519737            1387 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129423 [Amblyomma americanum].
ACCESSION   XP_077519737
VERSION     XP_077519737.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663611.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1387
                     /product="uncharacterized protein LOC144129423"
                     /calculated_mol_wt=157207
     Region          423..983
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1387
                     /gene="LOC144129423"
                     /coded_by="XM_077663611.1:9..4172"
                     /db_xref="GeneID:144129423"
ORIGIN      
        1 mhivlkwcdq cqnasnngaa agksgppeww laseeleefc dpystqiate hkdvpvnafa
       61 lgcflgyntf vahfdstytm ppppsplssa ksssslsgks msvrrgkkgr avkssspsdg
      121 tsssaktscp nseltssegf kvtqcnahfe hcynglylyf vdvrkrsstl frllvrysti
      181 criilrktsp tstivylqll hppllykvca gnsatsqcnr egdylpqfdl sdttpwdrav
      241 efgegpfkcg sdvlgrsrmm rltlsgprhw aplcnlilrt rknaqvfyap vrelharefk
      301 avarpsvqdf gvvyallsvw sesfqvtdel amdaqscret llelsaraee cpraleealf
      361 eiyfslsqgk vfwfrsaleh lyfryrahrq ridvftgsvi aqelpshmvk vhkviltptr
      421 rvymppqlvc ksrllqhcdp dyalrivvrd ddcklisfsl gackddflsa vikpplssgl
      481 kiggrrfmfl gcstsqlrsh gvwfyahdnq grtaeqirdg igdlstivsv pkfmarlgqa
      541 fsqslglltv psqntsvepd irhaapgrss dgshreyifs dgigrishgl lrkvhhalel
      601 eegeepcavq iryggckgml lldptlsgcr ivfresmrkf hsdhsdlyvl ktskprvlyl
      661 nrpmitileq sgikaevflm lqnkildsfi dsmmdpheaa hvlrsycalr lpykelagvg
      721 idltvepffr alvravnkkv lkelrtkari lvppnygrtm fgvldetgtl eygqvfvqys
      781 ndmlrykvnd patilegdvi vtknpcmnpg dirkleavnv pqlhhvrdci vfpqkgerph
      841 pdemagsdld gdeysvlwye dlifnnncnp mhyhsdppke rkasigvqdm vdffcqyikg
      901 dkiglianah lvwadildsg inshrcrela rkcavnldfa kcgdlkgfqn sekppmypdf
      961 mekldtknty csrkvlgqly rnckkvelst eglevveesl pdprlllegr eqflkeatsa
     1021 ykryakkira llksyriete seafsgavsk lskymkendp tdmamvlesq vehvvrrtre
     1081 effseqldea heklkasawy qvtyelqsse ggiqsfpwvv ydvlmrivvn tssclpvpas
     1141 rnsfcqrlga lllglphpgg gdqdgtqhgd tqvltnllrl myewidssre flfvknteel
     1201 gvyksimrea cfkvsrsisr dmpphklvil clrfacawcl kifqggsdge viskecrrry
     1261 rlghlalitl nrlsmsgnla ylrrapegcp steliriyin redeeffeil rryediikki
     1321 lmdwsgvedi qcdlktdrmd ewflqlmvtg srwalerlke ivvypsfrev lllaferekn
     1381 aiggtle
//
LOCUS       XP_077519863             320 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129585 [Amblyomma americanum].
ACCESSION   XP_077519863
VERSION     XP_077519863.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663737.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 22% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..320
                     /product="uncharacterized protein LOC144129585"
                     /calculated_mol_wt=35205
     Region          23..111
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..320
                     /gene="LOC144129585"
                     /coded_by="XM_077663737.1:1..963"
                     /db_xref="GeneID:144129585"
ORIGIN      
        1 maasvqgmea aaavsqqsss qrrvnwpdvt taaliriwed qlpalrgntr nariyaamaa
       61 elnaglapge grytakqvkq kmdnlnkkyr qlrrtgtatg spgvpwqfyw qlhsflgslp
      121 inddgrvees lelpvvnevp gtaevlasge deplgdradp qllsmsplav lpstsgnmgc
      181 snrgetsggg rsrrkrdsss vvhqllalhk deaaqavkae krahkfrkkm lrmtqegndi
      241 nanlvrlldk yikskedken ndrkikrtgt ttgsggqdwp fywhlhqflg slpmndemli
      301 eenvevpvva empedaelvs
//
LOCUS       XP_077519922             428 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129665 [Amblyomma americanum].
ACCESSION   XP_077519922
VERSION     XP_077519922.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663796.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..428
                     /product="uncharacterized protein LOC144129665"
                     /calculated_mol_wt=46755
     Region          <308..366
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..428
                     /gene="LOC144129665"
                     /coded_by="XM_077663796.1:43..1329"
                     /db_xref="GeneID:144129665"
ORIGIN      
        1 mfklsrirgf vkrkliqqal vpiiwaarrl qrqqqvverr pnpgleeeqv edgdlegkir
       61 lqgrresnml gpsfkyylkk svcvrahqql raqvggpqsv riiwrpvgpr tpprpplplp
      121 grkknfavqs kidsglqrkr aaaaayefql atkdfsvhqi iakcfaalpm stafiptgqp
      181 taekgngese eeassssnpd gsrgnrarqg rragaargar pgreapaesg aepaggleds
      241 lkgaaapggs rglparrpeg rrgrlrggri argqlhadia tsvgnedeva aptsslarra
      301 rqagqaarfp qgeknkivrq hwianlerkd fnrgsssrvc svhfidgrpt kdnpyptlhl
      361 gtptvhhnei rklarhdvtp lepdacstae plshhssdeq dnesasadhh lpinvgtedl
      421 shryskvv
//
LOCUS       XP_077519989             227 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129761 [Amblyomma americanum].
ACCESSION   XP_077519989
VERSION     XP_077519989.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663863.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 35% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..227
                     /product="uncharacterized protein LOC144129761"
                     /calculated_mol_wt=24967
     Region          <175..226
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     CDS             1..227
                     /gene="LOC144129761"
                     /coded_by="XM_077663863.1:302..985"
                     /db_xref="GeneID:144129761"
ORIGIN      
        1 mlpdyweapg sgrqdlledp asweeqrdee pcdgsgivar adtaladver eafeagweth
       61 resppsssad alpdhweapa ssgrdlreep ssleeqheek pydrsgmvah agctsdtawa
      121 hvksevksgd aslvgksmhg lgevhsqgcp agsvhvecaa qgtalrhvrr cedffvvdgh
      181 pqernhwmnd vnyspskrrq nlvallvngd iyyrtirnvg peeelll
//
LOCUS       XP_077520072             279 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129856 [Amblyomma americanum].
ACCESSION   XP_077520072
VERSION     XP_077520072.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663946.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 48% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..279
                     /product="uncharacterized protein LOC144129856"
                     /calculated_mol_wt=30498
     CDS             1..279
                     /gene="LOC144129856"
                     /coded_by="XM_077663946.1:1..840"
                     /db_xref="GeneID:144129856"
ORIGIN      
        1 matppkrprm svaqglllie lleqhpplar aaselspeyp aarrgelwer iaaalnregp
       61 avksaelwgt sggrlsgrag hvvqlvgpeq aspphslspp rghrasvgsv pgtsglqreh
      121 rsaaddtnms wagasettpl elleeeqqhq haeiastqqp talstlpsfa sppsppvpgr
      181 pvwrrrqrde evadllretr rtadltearn rrdaefqarl lehlneaaad rrqltatvad
      241 laaavrdtre qsvrlheqlv vavsflllml hyqihppph
//
LOCUS       XP_077520228             192 aa            linear   INV 25-NOV-2025
DEFINITION  chitinase-3-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077520228
VERSION     XP_077520228.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664102.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 16% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..192
                     /product="chitinase-3-like protein 1"
                     /calculated_mol_wt=21405
     Region          <19..150
                     /region_name="GH18_chitinase-like"
                     /note="The GH18 (glycosyl hydrolase, family 18) type II
                     chitinases hydrolyze chitin, an abundant polymer of
                     beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a
                     major component of the cell wall of fungi and the
                     exoskeleton of arthropods. Chitinases have...; cl10447"
                     /db_xref="CDD:471972"
     CDS             1..192
                     /gene="LOC144130048"
                     /coded_by="XM_077664102.1:1..579"
                     /db_xref="GeneID:144130048"
ORIGIN      
        1 mhdaqvease aflgelrsrk rvalelslfg yayrlrntgs yelrasvrgp apprpfider
       61 gllvyfeicl efssdlsvpl ynkeadcksa vnrdkgqwig ydnkatilnn vlfakrmrmg
      121 siavvtvdmd dyngncgqrn vllgqlhnal neldrpikrl sanlslsesa sssttapavv
      181 aqwnvqerym hc
//
LOCUS       XP_077520353             192 aa            linear   INV 25-NOV-2025
DEFINITION  chitinase-3-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077520353
VERSION     XP_077520353.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664227.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..192
                     /product="chitinase-3-like protein 1"
                     /calculated_mol_wt=21435
     Region          <19..150
                     /region_name="GH18_chitinase-like"
                     /note="The GH18 (glycosyl hydrolase, family 18) type II
                     chitinases hydrolyze chitin, an abundant polymer of
                     beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a
                     major component of the cell wall of fungi and the
                     exoskeleton of arthropods. Chitinases have...; cl10447"
                     /db_xref="CDD:471972"
     CDS             1..192
                     /gene="LOC144130235"
                     /coded_by="XM_077664227.1:1..579"
                     /db_xref="GeneID:144130235"
ORIGIN      
        1 mhdaqvease aflgelrsrk rvalelslfg yayrlrntgs yelqasvrgp apprpfider
       61 gllvyfeicl efssdlsvpl ynkeadckia vnrdkgqwig ydnnatilnn vlfakrmrmg
      121 siavvtvdmd dyngncgqrn vllgqlhnal neldrpikrl sanlspfesa sssttapaav
      181 aqwneqerym hc
//
LOCUS       XP_077520423            1470 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130334 [Amblyomma americanum].
ACCESSION   XP_077520423
VERSION     XP_077520423.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664297.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1470
                     /product="uncharacterized protein LOC144130334"
                     /calculated_mol_wt=166217
     Region          506..1066
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1470
                     /gene="LOC144130334"
                     /coded_by="XM_077664297.1:1..4413"
                     /db_xref="GeneID:144130334"
ORIGIN      
        1 mapttafcar dredncrema lrvhcldsgw gtptasqlrr llglqcavgq vvagdcgeva
       61 vyesptwtan vtlqlerspr dsfmhivlkw cdqcqnasnn gaaagksgpp ewwlaseele
      121 vfcdpystqi atehkdvpvn afalgcflgy ntfvahfdst ytmppppspl ssakssssls
      181 gksmsvkrgk kgravksssp sdgtsssakt pcpnseltss egfkvtqcna hfehcyngly
      241 lyfvdvrkrs stlfrllvry sticriilrk tsptstivyl qllhppllyk vcagnsatsq
      301 cnregdylpq fdlsdttpwd ravefgegpf kcgsdvlgrs rmmrltlsgp rhwaplcnli
      361 lrtrknaqvf yapvrellar elkavarpsv qdfgvvyall svwsesfqvt delamdaqsc
      421 retllelsar aeecpralee alfeiyfsls qgkvfwfrsa lehlyfryra hrqridvftg
      481 sviaqelpsh mvkvrkvilt ptrrvymppq lvcksrllqh cdpdyalriv vrdddcklis
      541 fslgackddf lsavikppls sglkiggrrf mflgcstsql rshgvwfyah dnqgrtaeqi
      601 rdgigdlsti vsvpkfmarl gqafsqslgl lmvpsqntsv epdirhaapg rssdgshrey
      661 ifsdgigris hgllrkvhha leleegeepc avqiryggck gmllldptls gcrivfresm
      721 rkfhsdhsdl yvlktskprv lylnrpmiti leqsgikaev flmlqnkild sfidsmmdph
      781 eaahvlrsyc alrlpykela gvgidltvep ffralvcavn kkvlkelrtk arilvppnyg
      841 rtmfgvldet gtleygqvfv qysndmlryk vndpatileg dvivtknpcm npgdirklea
      901 vnvpqlhhvr dcivfpqkge rphpdemags dldgdeysvl wyedlifnnn cnpmhyhsdp
      961 pkerkasigv qdmvdffcqy ikgdkiglia nahlvwadil dsginshrcr elarkcavnl
     1021 dfakcgdlkg fqnsekppmy pdfmekldtk ntycsrkvlg qlyrnckkve lsteclevve
     1081 eslpdprlll egreqflkea tsaykryakk irallksyri eteseafsga vsklskymke
     1141 ndptdmamvl esqvehvvrr treeffseql deaheklkas awyqvtyelq sseggiqsfp
     1201 wvvydvlmri vvntssclpv pasrnsfcqr lgalllglph pgggdqdgtq hgdtqvltnl
     1261 lrlmydwids sreflfvknt eelgvyksim reacfkvsrs isrdmpphkl vilclrfaca
     1321 wclkifqggs dgeviskecr rryrlghlal itlnrlsmsg nlaylrrape gcpsteliri
     1381 yinredeeff eilrryedii kkimmdwsgv ediqcdlktd rmdewflqlm vtgsrwaler
     1441 lkeivvypsf revlllafer eknaiggtle
//
LOCUS       XP_077520521             627 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130440 [Amblyomma americanum].
ACCESSION   XP_077520521
VERSION     XP_077520521.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664395.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..627
                     /product="uncharacterized protein LOC144130440"
                     /calculated_mol_wt=69996
     Region          4..94
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          262..460
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..627
                     /gene="LOC144130440"
                     /coded_by="XM_077664395.1:58..1941"
                     /db_xref="GeneID:144130440"
ORIGIN      
        1 mgrccvpgcr gnydtgpkvr vfafpkdasr reswiraipr kdftpsvhsk vcelhfqend
       61 lirklshfda asgktltvei ervhlvsdav psifpncpay lstnhtrres paskrarmed
      121 ealgkaikss metkkdeeqr iaiasfqdlk hklsgignsd fwtisvnsrc vmflrieenp
      181 sphvrfsvtv fadlslsvsa gmiklnslpc ggaipeavrd tstlsvlleq lekhmtetse
      241 ettasktttv lqhigsllne lteesdatke lsalirllhe qitltlagpi rrysadtlvf
      301 ssilhsisph aynfarsasm ltlphpstlr rvcskhggdp mkeqneahfl syirervkfm
      361 eshektvtlm ideihikpyf dykggsivgs sansqeaatt ahvfmiqsll sankdvihil
      421 pvakmnseil hdyckriile meslglkvia vitdnnslnr kmmslfsenr evsivyphpa
      481 dksrplfyvv dtvhvlkcir nnwinqknqg tclyfpemsl sgvlpdgppr mkaasfgavr
      541 qlyalektsl lklgyklnsk avyptpmerq nvklaldvin pfvsnalemr gkalkimnss
      601 stalfiniia twwkivnnav nkskkpp
//
LOCUS       XP_077520555             192 aa            linear   INV 25-NOV-2025
DEFINITION  chitinase-3-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077520555
VERSION     XP_077520555.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664429.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..192
                     /product="chitinase-3-like protein 1"
                     /calculated_mol_wt=21385
     Region          <19..150
                     /region_name="GH18_chitinase-like"
                     /note="The GH18 (glycosyl hydrolase, family 18) type II
                     chitinases hydrolyze chitin, an abundant polymer of
                     beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a
                     major component of the cell wall of fungi and the
                     exoskeleton of arthropods. Chitinases have...; cl10447"
                     /db_xref="CDD:471972"
     CDS             1..192
                     /gene="LOC144130722"
                     /coded_by="XM_077664429.1:1..579"
                     /db_xref="GeneID:144130722"
ORIGIN      
        1 mhdaqvease aflgelrsrk rvalelslfg yayrlrntgs yelrasvrgp apprpfider
       61 gllvyfeicl efssdlsvpl ynkeadckia vnrdkgqwig ydnnatilnn vlfakrmrmg
      121 siavvtvdmd dyngncgqrn vllgqlhnal neldrpikrl sanlspsesa sssttapaav
      181 aqwneqeryi hc
//
LOCUS       XP_077520559            1470 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130882 [Amblyomma americanum].
ACCESSION   XP_077520559
VERSION     XP_077520559.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664433.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1470
                     /product="uncharacterized protein LOC144130882"
                     /calculated_mol_wt=166369
     Region          506..1066
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1470
                     /gene="LOC144130882"
                     /coded_by="XM_077664433.1:1..4413"
                     /db_xref="GeneID:144130882"
ORIGIN      
        1 mapttafcar dredncrema lrvhcldsgw gtptasqlrr llglqcavgq vvagdcgeva
       61 vyesptwtan vtlqlerspr dsfmhivlkw cdqcqnasnn gaaagksgpp ewwlaseele
      121 efcdpystqi atehkdvpvn afalgcflgy ntfvahfdst ytmppppspl ssakssssls
      181 gksmsvkrgk kgravksssp sdgtsssakt scpnseltss egfkvtqcna hfehcyngly
      241 lyfvdvrkrs stlfrllvry sticriilrk tsptstivyl qllhppllyk vcagnsatsq
      301 cnregdylpq fdlsdttpwd ravefgegpf kcgadvlgrs rmmrltlsgp rhwaplcnli
      361 lrtrknaqvf yapvrelhar efkavarpsv qdfgvvyall svwsesfqvt delamdaqsc
      421 retllelsar aeecpralee alfeiyfsls qgkvfwfrsa lehlyfryra hrqridvftg
      481 sviaqelpsh ivkvrkvilt ptrrvymppq lvcksrllqh cdpdyalriv vrdddcklis
      541 fslgackddf lsavikppls sglkiggrrf mflgcstsql rshgvwfyah dnqgrtaeqi
      601 rdgigdlsti vsvpkfmarl gqafsqslgl ltvpsqntsv epdirhaapg rssdgshrey
      661 ifsdgigris hgllrkvhha leleeseepc avqiryggck gmllldptls gcrivfresm
      721 rkfysdhsdl yvlktskprv lylnrpmiti leqsgikaev flmlqnkild sfidsmmdph
      781 eaahvlrsyc alrlpykela gvgidltvep ffralvravn kkvlkelrtk arilvppnyg
      841 rtmfgvldet gtleygqvfv qysndmlryk vndpatileg dvivtknpcm npgdirklea
      901 vnvpqlhhvr dcivfpqkge rphpdemags dldgdeysvl wyedlifnnn cnpmhyhsdp
      961 pkerkasigv qdmvdffcqy ikgdkiglia nahlvwadil dsginshrcr elarkcavnl
     1021 dfakcgdlkg fqnseqppmy pdfmekldtk ntycsrkvlg qlyrnckkve lsteclevve
     1081 eslpdprlll egreqflkea msaykryakk irallksyri eteseafsga vsklskymke
     1141 ndptdmamvl esqvehvvrr treeffseql deaheklkas awyqvtyelq sseggiqsfp
     1201 wvvydvlmri vvntssclpv pasrnsfcqr lgalllglph pgggdqdgtq hgdtqvltnl
     1261 lrlmydwids sreflfvknt eelgvyksim reacfkvsrs isrdmpphkl vilclrfaca
     1321 wclkifqggs dgeviskecr rryrlghlal itlnrlsmsg nlaylrrape gcpsteliri
     1381 yinredeeff eilrryedii kkimmdwsgv ediqcdlktd rmdewflqlm vtgsrwaler
     1441 lkeivvypsf rkvlllafer eknaiggtle
//
LOCUS       XP_077520568             192 aa            linear   INV 25-NOV-2025
DEFINITION  chitinase-3-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077520568
VERSION     XP_077520568.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664442.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..192
                     /product="chitinase-3-like protein 1"
                     /calculated_mol_wt=21104
     Region          <19..150
                     /region_name="GH18_chitinase-like"
                     /note="The GH18 (glycosyl hydrolase, family 18) type II
                     chitinases hydrolyze chitin, an abundant polymer of
                     beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a
                     major component of the cell wall of fungi and the
                     exoskeleton of arthropods. Chitinases have...; cl10447"
                     /db_xref="CDD:471972"
     CDS             1..192
                     /gene="LOC144131205"
                     /coded_by="XM_077664442.1:1..579"
                     /db_xref="GeneID:144131205"
ORIGIN      
        1 mhdaqvease aflgelrsrk rvalelslfg yayrlrntgs yelrasvrgp apprpfider
       61 gllvyfeicl efssdlsvpl ynkgadckia vnrdkgqwig ydnnatilnn vlfakrmrmg
      121 siavvtvdmd dyngncgqrn vllgqlhnal neldrpikrl sanlspsesa sssttapaav
      181 aqcvhgniri qe
//
LOCUS       XP_077520587             244 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144131686 [Amblyomma americanum].
ACCESSION   XP_077520587
VERSION     XP_077520587.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664461.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..244
                     /product="uncharacterized protein LOC144131686"
                     /calculated_mol_wt=26297
     Region          <12..191
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(54..59,107..108,154,156..157)
                     /site_type="active"
                     /db_xref="CDD:238827"
     Site            order(54..59,107,156)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238827"
     Site            108
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238827"
     CDS             1..244
                     /gene="LOC144131686"
                     /coded_by="XM_077664461.1:1..735"
                     /db_xref="GeneID:144131686"
ORIGIN      
        1 maeraqilge lqngfrpnhr lednlfvvts sieiaakekr elvlgfsgys pgiplsgtqs
       61 tvggpgdtgy lgatttlvea lytetqavih wrasttrpiy vyiglrqgcp mppiqfklft
      121 srlerrlvec gvgfnltcmk dgttrthrlp vllyaddivl lvesvgdlqs lvdmcssegd
      181 dlglrfnaak slsaatpppl lsdhleqltr tlvttlqavl qlfpvasqpp qcgagpaqqp
      241 nhhg
//
LOCUS       XP_077520602             185 aa            linear   INV 25-NOV-2025
DEFINITION  myb/SANT-like DNA-binding domain-containing protein 1 [Amblyomma
            americanum].
ACCESSION   XP_077520602
VERSION     XP_077520602.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664476.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 31% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..185
                     /product="myb/SANT-like DNA-binding domain-containing
                     protein 1"
                     /calculated_mol_wt=19964
     Region          23..111
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..185
                     /gene="LOC144132007"
                     /coded_by="XM_077664476.1:1..558"
                     /db_xref="GeneID:144132007"
ORIGIN      
        1 maasvqgmea aaavsqqsss qrrvnwpdvt taaliriwed qlpalrgntr nariyaamaa
       61 elnaglapge grytakqvkq kmdnlnkkyr qlrrtgtatg spgvpwqfyw qlhsflgslp
      121 inddgrvees lelpvinevp gtaevlasge deplgdradp qllsmsplav lpilllaikl
      181 lamsd
//
LOCUS       XP_077520715             297 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132262 [Amblyomma americanum].
ACCESSION   XP_077520715
VERSION     XP_077520715.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664589.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 15% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..297
                     /product="uncharacterized protein LOC144132262"
                     /calculated_mol_wt=32016
     Region          <101..159
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..297
                     /gene="LOC144132262"
                     /coded_by="XM_077664589.1:1..894"
                     /db_xref="GeneID:144132262"
ORIGIN      
        1 mieqylkrve gvrpgreapa esgaepaggl edslkgaaap ggsrglparr pegrrgrlrg
       61 griargqlha diamsvgned evaaptssla rrarqagqaa rfpqgeknki vrqhwianle
      121 rkdfnrgsss rvcsvhfidg rptkdnpypt lhlgtptvhh ngirklachd vtplepdacs
      181 taeplshhss deqdkesasa dhhlpinvgt edlshrrega qdlvdgclac gcrckiaets
      241 dkgtqvepat vrdhlyatpr kqapvtadsa cqtifsaysa lapshfrffy rcvkssi
//
LOCUS       XP_077520791             106 aa            linear   INV 25-NOV-2025
DEFINITION  histone-lysine N-methyltransferase set-17-like [Amblyomma
            americanum].
ACCESSION   XP_077520791
VERSION     XP_077520791.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664665.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..106
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..106
                     /product="histone-lysine N-methyltransferase set-17-like"
                     /calculated_mol_wt=11778
     Region          6..105
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(12..13,68..72)
                     /site_type="other"
                     /note="SAM binding site [polypeptide binding]"
                     /db_xref="CDD:380914"
     CDS             1..106
                     /gene="LOC144132320"
                     /coded_by="XM_077664665.1:1..321"
                     /db_xref="GeneID:144132320"
ORIGIN      
        1 masgkstvkg aplgvftlka lpkglhfgpy hgvkvdsien ggctwpvrrc edffvvdghp
       61 qernhwmndv nyspskrrqn lvallvngdi yyrtirnvgp eeelll
//
LOCUS       XP_077520902             279 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132381 [Amblyomma americanum].
ACCESSION   XP_077520902
VERSION     XP_077520902.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664776.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 48% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..279
                     /product="uncharacterized protein LOC144132381"
                     /calculated_mol_wt=30498
     CDS             1..279
                     /gene="LOC144132381"
                     /coded_by="XM_077664776.1:1..840"
                     /db_xref="GeneID:144132381"
ORIGIN      
        1 matppkrprm svaqglllie lleqhpplar aaselspeyp aarrgelwer iaaalnregp
       61 avksaelwgt sggrlsgrag hvvqlvgpeq aspphslspp rghrasvgsv pgtsglqreh
      121 rsaaddtnms wagasettpl elleeeqqhq haeiastqqp talstlpsfa sppsppvpgr
      181 pvwrrrqrde evadllretr rtadltearn rrdaefqarl lehlneaaad rrqltatvad
      241 laaavrdtre qsvrlheqlv vavsflllml hyqihppph
//
LOCUS       XP_077521014             167 aa            linear   INV 25-NOV-2025
DEFINITION  histone-lysine N-methyltransferase set-17-like [Amblyomma
            americanum].
ACCESSION   XP_077521014
VERSION     XP_077521014.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664888.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..167
                     /product="histone-lysine N-methyltransferase set-17-like"
                     /calculated_mol_wt=18242
     Region          39..142
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(39,93..98,109..111,132..135)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Site            order(39,94..98,134)
                     /site_type="other"
                     /note="SAM binding site [polypeptide binding]"
                     /db_xref="CDD:380914"
     CDS             1..167
                     /gene="LOC144132438"
                     /coded_by="XM_077664888.1:1..504"
                     /db_xref="GeneID:144132438"
ORIGIN      
        1 magwvsfrlp vqsqdsslvg tslfeflasg kstvkgvplg vftlkalpkg lhlgpyhavk
       61 vdsienggct wpvrrcgeff vvdgrnqdrs hwmnninysp skckqnlval lfnediyyra
      121 lrnigpdeel lvgysasfak sllgnprgrg alkegeekli lqslagr
//
LOCUS       XP_077521224             192 aa            linear   INV 25-NOV-2025
DEFINITION  chitinase-3-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077521224
VERSION     XP_077521224.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665098.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 16% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..192
                     /product="chitinase-3-like protein 1"
                     /calculated_mol_wt=21431
     Region          <19..150
                     /region_name="GH18_chitinase-like"
                     /note="The GH18 (glycosyl hydrolase, family 18) type II
                     chitinases hydrolyze chitin, an abundant polymer of
                     beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a
                     major component of the cell wall of fungi and the
                     exoskeleton of arthropods. Chitinases have...; cl10447"
                     /db_xref="CDD:471972"
     CDS             1..192
                     /gene="LOC144132574"
                     /coded_by="XM_077665098.1:1..579"
                     /db_xref="GeneID:144132574"
ORIGIN      
        1 mhdaqvease aflgelrsrk rvalelslfg yayrlrntgs yelrasvrgp apprpfider
       61 gllvyfeicl efssdlsvpl ynkeadckia vnrdkgqwig ydnkatilnn vlfakrmrmg
      121 siavvtvdmd dyngncgqrn vllgqlhnal neldrpikrl sanlslsesa sssttapavv
      181 aqwnvqerym hc
//
LOCUS       XP_077521349             382 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132633, partial [Amblyomma
            americanum].
ACCESSION   XP_077521349
VERSION     XP_077521349.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665223.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: incomplete on the carboxy end.
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..>382
                     /product="uncharacterized protein LOC144132633"
     Region          <1..94
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..382
                     /gene="LOC144132633"
                     /coded_by="XM_077665223.1:1..>1146"
                     /db_xref="GeneID:144132633"
ORIGIN      
        1 mvdffcqyik gdkigliana hlvwadilds ginshrcrel arkcavnldf akcgdlkgfq
       61 nsekppmypd fmekldtknt ycsrkvlgql yrnckkvels seclevvees lphprllleg
      121 reqflkeats aykryakkir allksyriet eseafsgavs klskymkend ptdmsmvles
      181 qvehvvrrtr eeffseqlee ahenlkasaw yqvtyelqss eggiqsfpwv vcdvlmrivv
      241 ntssclpvpa srnsfcqrlg alllglphpg ggdqdgtqhg dtqvltnllr lmydwidssr
      301 eflfvkntee lgvyksimre acfkvsrsis rdmpphklvi lclrfacawc lkifqggsdg
      361 eviskecrhr yrlghlalim ln
//
LOCUS       XP_077521444             489 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132695 [Amblyomma americanum].
ACCESSION   XP_077521444
VERSION     XP_077521444.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665318.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 15% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..489
                     /product="uncharacterized protein LOC144132695"
                     /calculated_mol_wt=54936
     Region          16..488
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..489
                     /gene="LOC144132695"
                     /coded_by="XM_077665318.1:1..1470"
                     /db_xref="GeneID:144132695"
ORIGIN      
        1 mvkvrkvilt ptrrvymppq lvcksrllqh cdpdyalriv vrdddcklis fslgackddf
       61 lsavikppls sglkiggrrf mflgcstsql rshgvwfyah dnqgrtaeqi rdgigdlsti
      121 vsvpkfmarl gqafsqslgl ltvpsqntsv epdirhaapg rssdgshrey ifsdgigris
      181 hgllrkvhha leleegeepc avqiryggck gmllldptls gcrivfresm rkfhsdhsdl
      241 yvlktskprv lylnrpmiti leqsgikaev flmlqnkild sfidsmmdph eaahvlcsyc
      301 awrlpykela gvgidltvep ffralvravn kkvlkelrtk arilvppnyg rtmfgvldet
      361 gtleygqvfv qysndmlryk vndpatileg dvivtknpcm npgdirklea vnvpqlhhvr
      421 dcivfpqkge rphpdemags dldgdeysvl wyedlifnnn cnpmhyhsdp pkerkasigv
      481 sniayfvvs
//
LOCUS       XP_077521561             238 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132755 [Amblyomma americanum].
ACCESSION   XP_077521561
VERSION     XP_077521561.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665435.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..238
                     /product="uncharacterized protein LOC144132755"
                     /calculated_mol_wt=25400
     CDS             1..238
                     /gene="LOC144132755"
                     /coded_by="XM_077665435.1:336..1052"
                     /db_xref="GeneID:144132755"
ORIGIN      
        1 meppakpflp gpmapttafc ardredncre malrvhclds gwgtptasql rrllglqcav
       61 gqvvagdcge vavyesptwt anvtlqlers pkdsfmhivl kwcdqcqnas nngaaagksg
      121 ppewwlasee leescdpyst qiatehkdvp vhafalgcfl gyntfvahfd stytmpppps
      181 plssakssss lsgksmsvkr gkkgrafkss spsdgtsssa ktscpnselt ssegfkvr
//
LOCUS       XP_077521797             192 aa            linear   INV 25-NOV-2025
DEFINITION  chitinase-3-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077521797
VERSION     XP_077521797.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665671.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..192
                     /product="chitinase-3-like protein 1"
                     /calculated_mol_wt=21417
     Region          <19..150
                     /region_name="GH18_chitinase-like"
                     /note="The GH18 (glycosyl hydrolase, family 18) type II
                     chitinases hydrolyze chitin, an abundant polymer of
                     beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a
                     major component of the cell wall of fungi and the
                     exoskeleton of arthropods. Chitinases have...; cl10447"
                     /db_xref="CDD:471972"
     CDS             1..192
                     /gene="LOC144132905"
                     /coded_by="XM_077665671.1:1..579"
                     /db_xref="GeneID:144132905"
ORIGIN      
        1 mhdaqvease aflgelrsrk rvalelslfg yayrlrntgs yelrasvrgp apprpfider
       61 gllvyfeicl efssdlsvpl ynkeadckia inrdkgqwig ydnnatilnn vlfakrmrmg
      121 siavvtvdmd dyngncgqrn vllgqlhnal neldrpikrl sanlspsesa sssttapaav
      181 aqwneqerym hc
//
LOCUS       XP_077521917             411 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132979 [Amblyomma americanum].
ACCESSION   XP_077521917
VERSION     XP_077521917.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665791.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 25% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..411
                     /product="uncharacterized protein LOC144132979"
                     /calculated_mol_wt=45198
     CDS             1..411
                     /gene="LOC144132979"
                     /coded_by="XM_077665791.1:1..1236"
                     /db_xref="GeneID:144132979"
ORIGIN      
        1 mapttafcar dredncrema lrvhcldsgw gtptasqlrr llglqcavgq vvagdcgeva
       61 vyesptwtan vtlqlerspr dsfmhivlkw cdqcqnasnn gaaagksgpp ewwlaseele
      121 efcdpystqi atehkdvpvn afalgcflgy ntfvahfdst ytmppppspl ssakssssls
      181 gksmsvkrgk kgravksssp sdgtsssakt pcpnseltss egfkvtqcna hfehcsngly
      241 lyfvdvrkrs stlfrllvry sticriilrk tsptstivyl qllhppllyk vcagnsatsq
      301 cnregdylpq fdlsdttpwd ravefgegpf kcgadvlgrs rmmrltlsgp rhwaplcnli
      361 lrtrknaqvf yapvrelhar efkavarpsv qdfgvvyall svwsesfqvg i
//
LOCUS       XP_077522037             215 aa            linear   INV 25-NOV-2025
DEFINITION  inactive selenide, water dikinase-like protein [Amblyomma
            americanum].
ACCESSION   XP_077522037
VERSION     XP_077522037.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665911.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 15% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..215
                     /product="inactive selenide, water dikinase-like protein"
                     /calculated_mol_wt=23271
     Region          1..199
                     /region_name="PurM-like"
                     /note="AIR (aminoimidazole ribonucleotide) synthase
                     related protein. This family includes Hydrogen
                     expression/formation protein HypE, AIR synthases, FGAM
                     (formylglycinamidine ribonucleotide) synthase and
                     Selenophosphate synthetase (SelD). The N-terminal
                     domain...; cl10019"
                     /db_xref="CDD:471963"
     CDS             1..215
                     /gene="LOC144133046"
                     /coded_by="XM_077665911.1:1..648"
                     /db_xref="GeneID:144133046"
ORIGIN      
        1 mdssgvplgd sgyslvqtid yfypsvedpy iagkiacanv lsdlyalgmt rcdnvlmvma
       61 vstkltekqr divvpllirg sqgrigpccs hgclselmpd naqpgdvlvl tkplctqvav
      121 nahqwlekpe rhayllaahg kgdsdnlatn ikdcrarlmh khgchgatgv tgfgllghtq
      181 nlvqmqqrsv rfvihtlpir anflpcwppr appsn
//
LOCUS       XP_077522260             192 aa            linear   INV 25-NOV-2025
DEFINITION  chitinase-3-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077522260
VERSION     XP_077522260.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666134.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..192
                     /product="chitinase-3-like protein 1"
                     /calculated_mol_wt=21417
     Region          <19..150
                     /region_name="GH18_chitinase-like"
                     /note="The GH18 (glycosyl hydrolase, family 18) type II
                     chitinases hydrolyze chitin, an abundant polymer of
                     beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a
                     major component of the cell wall of fungi and the
                     exoskeleton of arthropods. Chitinases have...; cl10447"
                     /db_xref="CDD:471972"
     CDS             1..192
                     /gene="LOC144133203"
                     /coded_by="XM_077666134.1:1..579"
                     /db_xref="GeneID:144133203"
ORIGIN      
        1 mhdaqlease aflgelrsrk rvalelslfg yayrlrntgs yelrasvrgp apprpfider
       61 gllvyfeicl efssdlsvpl ynkeadckia vnrdkgqwig ydnnatilnn vlfakrmrmg
      121 siavvtvdmd dyngncgqrn vllgqlhnal neldrpikrl sanlspsesa sssttapaav
      181 aqwneqerym hc
//
LOCUS       XP_077522384            1470 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133269 [Amblyomma americanum].
ACCESSION   XP_077522384
VERSION     XP_077522384.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666258.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1470
                     /product="uncharacterized protein LOC144133269"
                     /calculated_mol_wt=166203
     Region          506..1066
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1470
                     /gene="LOC144133269"
                     /coded_by="XM_077666258.1:1..4413"
                     /db_xref="GeneID:144133269"
ORIGIN      
        1 mapttafcar dredncrema lrvhcldsgw gtptasqlrr llglqcavgq vvagdcgeva
       61 vyesptwtan vtlqlerspr dsfmhivlkw cdqcqnasnn gaaagksdpp ewwlaseele
      121 vfcdpystqi atehkdvpvn afalgcflgy ntfvahfdst ytmppppspl ssakssssls
      181 gksmsgkrgk kgravksssp sdgtsssakt pcpnseltss egfkvtqcna hfehcyngly
      241 lyfvdvrkrs stlfrllvry sticriilrk tsptstivyl qllhppllyk vcagnsatsq
      301 cnregdylpq fdlsdttpwd ravefgegpf kcgsdvlgrs rmmrltlsgp rhwaplcnli
      361 lrtrknaqvf yapvrellar elkavarpsv qdfgvvyall svwsesfqvt delamdaqsc
      421 retllelsar aeecpralee alfeiyfsls qgkvfwfrsa lehlyfryra hrqridvftg
      481 sviaqelpsh mvkvrkvilt ptrrvymppq lvcksrllqh cdpdyalriv vrdddcklis
      541 fslgackddf lsavikppls sglkiggrrf mflgcstsql rshgvwfyah dnqgrtaeqi
      601 rdgigdlsti vsvpkfmarl gqafsqslgl ltvpsqntsv epdirhaapg rssdgshrey
      661 ifsdgigris hgllrkvhha leleegeepc avqiryggck gmllldptls gcrivfresm
      721 rkfhsdhsdl yvlktskprv lylnrpmiti leqsgikaev flmlqnkild sfidsmmdph
      781 eaahvlrsyc alrlpykela gvgidltvep ffralvcavn kkvlkelrtk arilvppnyg
      841 rtmfgvldet gtleygqvfv qysndmlryk vndpatileg dvivtknpcm npgdirklea
      901 vnvpqlhhvr dcivfpqkge rphpdemags dldgdeysvl wyedlifnnn cnpmhyhsdp
      961 pkerkasigv qdmvdffcqy ikgdkiglia nahlvwadil dsginshrcr elarkcavnl
     1021 dfakcgdlkg fqnsekppmy pdfmekldtk ntycsrkvlg qlyrnckkve lsteclevve
     1081 eslpdprlll egreqflkea tsaykryakk irallksyri eteseafsga vsklskymke
     1141 ndptdmamvl esqvehvvrr treeffseql deaheklkas awyqvtyelq sseggiqsfp
     1201 wvvydvlmri vvntssclpv pasrnsfcqr lgalllglph pgggdqdgtq hgdtqvltnl
     1261 lrlmydwids sreflfvknt eelgvyksim reacfkvsrs isrdmpphkl vilclrfaca
     1321 wclkifqggs dgeviskecr rryrlghlal itlnrlsmsg nlaylrrape gcpsteliri
     1381 yinredeeff eilrryedii kkimmdwsgv ediqcdlktd rmdewflqlm vtgsrwaler
     1441 lkeivvypsf revlllafer eknaiggtle
//
LOCUS       XP_077522628             481 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133386 [Amblyomma americanum].
ACCESSION   XP_077522628
VERSION     XP_077522628.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666502.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..481
                     /product="uncharacterized protein LOC144133386"
                     /calculated_mol_wt=53589
     Region          4..94
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          262..460
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..481
                     /gene="LOC144133386"
                     /coded_by="XM_077666502.1:58..1503"
                     /db_xref="GeneID:144133386"
ORIGIN      
        1 mgrccvpgcr gnydtgpkvr vfafpkdasr reswiraipr kdftpsvhsk vcelhfqend
       61 lirklshfda asgktltvei ervhlvsdav psifpncpay lstnhtrres paskrarmed
      121 ealgkaikss metkkdeeqr iaiasfqdlk hklsgignsd fwtisvnsrc vmflrieenp
      181 sphvrfsvtv fadlslsvsa gmiklnslpc ggaipeavrd tstlsvlleq lekhmtetse
      241 ettasktttv lqhigsllne lteesdatke lsalirllhe qitltlagpi rrysadtlvf
      301 ssilhsisph aynfarsasm ltlphpstlr rvcskhggdp mkeqneahfl syirervkfm
      361 eshektvtlm ideihikpyf dykggsivgs sansqeaatt ahvfmiqsll sankdvihil
      421 pvakmnseil hdyckriile meslglkvia vitdnnslnr kmmslfsenr enavnkskkp
      481 p
//
LOCUS       XP_077522858             192 aa            linear   INV 25-NOV-2025
DEFINITION  chitinase-3-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077522858
VERSION     XP_077522858.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666732.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..192
                     /product="chitinase-3-like protein 1"
                     /calculated_mol_wt=21443
     Region          <19..150
                     /region_name="GH18_chitinase-like"
                     /note="The GH18 (glycosyl hydrolase, family 18) type II
                     chitinases hydrolyze chitin, an abundant polymer of
                     beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a
                     major component of the cell wall of fungi and the
                     exoskeleton of arthropods. Chitinases have...; cl10447"
                     /db_xref="CDD:471972"
     CDS             1..192
                     /gene="LOC144133533"
                     /coded_by="XM_077666732.1:1..579"
                     /db_xref="GeneID:144133533"
ORIGIN      
        1 mhdaqvease aflgelrsrk rvalelslfg yayrlrntgs yelrasvrgp apprpfider
       61 gllvyfeicl efssdlsvpl ynkeadckia vnrdkgqwig ydnnatilnn vlfakrmrmg
      121 siavvtvdmd dyngncdqrn vllgqlhnal neldrpikrl sanlspsesa sssttapaav
      181 aqwneqeryi hc
//
LOCUS       XP_077522937            1470 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133608 [Amblyomma americanum].
ACCESSION   XP_077522937
VERSION     XP_077522937.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666811.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1470
                     /product="uncharacterized protein LOC144133608"
                     /calculated_mol_wt=166344
     Region          506..1066
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1470
                     /gene="LOC144133608"
                     /coded_by="XM_077666811.1:1..4413"
                     /db_xref="GeneID:144133608"
ORIGIN      
        1 mapttafcar dredncrema lrvhcldsgw gtptasqlrr llglqcavgq vvagdcgeva
       61 vyesptwtan vtlqlerspr dsfmhivlkw cdqcqnasnn gaaagksgpp ewwlaseele
      121 efcdpystqi atehkdvpvn afalgcflgy ntfvahfdst ytmppppspl ssakssssls
      181 gksmsvkrgk kgravksssp sdgtsssakt scpnseltss egfkvtqcna hfehcyngly
      241 lyfvdvrkrs stlfrllvry sticriilrk tsptstivyl qllhppllyk vcagnsatsq
      301 cnregdylpq fdlsdttpwd ravefgegaf kcgadvlgrs rmmrltlsgp rhwaplcnli
      361 lrtrknaqvf yapvrelhar efkavarpsv qdfgvvyall svwsesfqvt delamdaqsc
      421 retllelsar aeecpralee alfeiyfsls qgkvfwfrsa lehlyfryra hrqridvftg
      481 sviaqelpsh ivkvrkvilt ptrrvymppq lvcksrllqh cdpdyalriv vrdddcklis
      541 fslgackddf lsavikppls sglkiggrrf mflgcstsql rshgvwfyah dnqgrtaeqi
      601 rdgigdlsti vsvpkfmarl gqafsqslgl ltvpsqntsv epdirhaapg rssdgshrey
      661 ifsdgigris hgllrkvhha leleeseepc avqiryggck gmllldptls gcrivfresm
      721 rkfysdhsdl yvlktskprv lylnrpmiti leqsgikaev flmlqnkild sfidsmmdph
      781 eaahvlrsyc alrlpykela gvgidltvep ffralvravn kkvlkelrtk arilvppnyg
      841 rtmfgvldet gtleygqvfv qysndmlryk vndpatileg dvivtknpcm npgdirklea
      901 vnvpqlhhvr dcivfpqkge rphpdemags dldgdeysvl wyedlifnnn cnpmhyhsdp
      961 pkerkasigv qdmvdffcqy ikgdkiglia nahlvwadil dsginshrcr elarkcavnl
     1021 dfakcgdlkg fqnseqppmy pdfmekldtk ntycsrkvlg qlyrnckkve lsteclevve
     1081 eslpdprlll egreqflkea msaykryakk irallksyri eteseafsga vsklskymke
     1141 ndptdmamvl esqvehvvrr treeffseql deaheklkas awyqvtyelq sseggiqsfp
     1201 wvvydvlmri vvntssclpv pasrnsfcqr lgalllglph pgggdqdgtq hgdtqvltnl
     1261 lrlmydwids sreflfvknt eelgvyksim reacfkvsrs isrdmpphkl vilclrfaca
     1321 wclkifqggs dgeviskecr rryrlghlal itlnrlsmsg nlaylrrape gcpsteliri
     1381 yinredeeff eilrryedii kkimmdwsgv ediqcdlktd rmdewflqlm vtgsrwaler
     1441 lkeivvypsf revlllafer eknaiggtle
//
LOCUS       XP_077523212             228 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134069 [Amblyomma americanum].
ACCESSION   XP_077523212
VERSION     XP_077523212.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667086.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..228
                     /product="uncharacterized protein LOC144134069"
                     /calculated_mol_wt=24897
     Region          <12..196
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(54..59,107..108,154,156..157)
                     /site_type="active"
                     /db_xref="CDD:238827"
     Site            order(54..59,107,156)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238827"
     Site            108
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238827"
     CDS             1..228
                     /gene="LOC144134069"
                     /coded_by="XM_077667086.1:1..687"
                     /db_xref="GeneID:144134069"
ORIGIN      
        1 maegaqilge lqngfrpnhr lednlfvvts sieiaakekr elvlgfsgys pgiplsgtqs
       61 tvggpgdtgy lgatttlvea lytetqavih wrasttrpiy vyiglrqgcp mppiqfmlft
      121 srlerrlvec gvgfnltcmk dgttrthrlp vllyaddivl lvesvgdlqs lvdmcssegd
      181 dlglrfnaak slsvrfqpea sedsgqethp cifriavsfg crptytwq
//
LOCUS       XP_077523291             446 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134173 [Amblyomma americanum].
ACCESSION   XP_077523291
VERSION     XP_077523291.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667165.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 34% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..446
                     /product="uncharacterized protein LOC144134173"
                     /calculated_mol_wt=49587
     CDS             1..446
                     /gene="LOC144134173"
                     /coded_by="XM_077667165.1:1..1341"
                     /db_xref="GeneID:144134173"
ORIGIN      
        1 msdlpmdght llrtprkpqe qcpvtplapg hycyfgladg llrllrlven ppaliplsfn
       61 idglplskss kmqlwpiqcl ahdcgdetpf lvgafagksk pdssveflrp fvselrtlls
      121 eglifngrvl hvimayaive flhheqvevv psswiddrqc awpdhakgdr ivslvrkaap
      181 pdsswkrfdi alkglfgayk lgrrkldksq ytselssdge pphkrtrrpp qawsedevsd
      241 tpttkvpyre nvpsvphdfp qgdfqelvri gdkdlfdkgh egearlsdrr dsvdedfkqh
      301 virllnilrf tqqqhgdmln klcevlpsqa iitkpllise pftsvddllk fnekldkenc
      361 tsagpsnles eavsaqpprk rprgkrnsth dseplspspr rrtssstrpp syksssapia
      421 atcaplgpal hpcaspaevs rlpaqd
//
LOCUS       XP_077523362             183 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134264 [Amblyomma americanum].
ACCESSION   XP_077523362
VERSION     XP_077523362.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667236.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="uncharacterized protein LOC144134264"
                     /calculated_mol_wt=18455
     CDS             1..183
                     /gene="LOC144134264"
                     /coded_by="XM_077667236.1:328..879"
                     /db_xref="GeneID:144134264"
ORIGIN      
        1 mstafiptgq ptaekgnges eeeassssnp dgsrgnrarq grragaarga rpgreapaes
       61 gaepaggldd slkgaaapgg srglparrpe grrgrlrggr iargqlhadi atsvgnedev
      121 aaptsslarr arqagqaarl rggriargql hadiatsvgn edevaaptss larrarqagq
      181 aar
//
LOCUS       XP_077523442             106 aa            linear   INV 25-NOV-2025
DEFINITION  histone-lysine N-methyltransferase set-17-like [Amblyomma
            americanum].
ACCESSION   XP_077523442
VERSION     XP_077523442.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667316.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..106
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..106
                     /product="histone-lysine N-methyltransferase set-17-like"
                     /calculated_mol_wt=11778
     Region          6..105
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(12..13,68..72)
                     /site_type="other"
                     /note="SAM binding site [polypeptide binding]"
                     /db_xref="CDD:380914"
     CDS             1..106
                     /gene="LOC144134358"
                     /coded_by="XM_077667316.1:1..321"
                     /db_xref="GeneID:144134358"
ORIGIN      
        1 masgkstvkg aplgvftlka lpkglhfgpy hgvkvdsien ggctwpvrrc edffvvdghp
       61 qernhwmndv nyspskrrqn lvallvngdi yyrtirnvgp eeelll
//
LOCUS       XP_077523536             279 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134466 [Amblyomma americanum].
ACCESSION   XP_077523536
VERSION     XP_077523536.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667410.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 48% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..279
                     /product="uncharacterized protein LOC144134466"
                     /calculated_mol_wt=30498
     CDS             1..279
                     /gene="LOC144134466"
                     /coded_by="XM_077667410.1:1..840"
                     /db_xref="GeneID:144134466"
ORIGIN      
        1 matppkrprm svaqglllie lleqhpplar aaselspeyp aarrgelwer iaaalnregp
       61 avksaelwgt sggrlsgrag hvvqlvgpeq aspphslspp rghrasvgsv pgtsglqreh
      121 rsaaddtnms wagasettpl elleeeqqhq haeiastqqp talstlpsfa sppsppvpgr
      181 pvwrrrqrde evadllretr rtadltearn rrdaefqarl lehlneaaad rrqltatvad
      241 laaavrdtre qsvrlheqlv vavsflllml hyqihppph
//
LOCUS       XP_077523614             153 aa            linear   INV 25-NOV-2025
DEFINITION  HEAT repeat-containing protein 1-like [Amblyomma americanum].
ACCESSION   XP_077523614
VERSION     XP_077523614.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667488.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..153
                     /product="HEAT repeat-containing protein 1-like"
                     /calculated_mol_wt=16514
     CDS             1..153
                     /gene="LOC144134572"
                     /coded_by="XM_077667488.1:1..462"
                     /db_xref="GeneID:144134572"
ORIGIN      
        1 mseevlhnvt svftykgasl lwqvdncsfq tvmptvktvm pallqactge sksaeaaesa
       61 vasvtrvfvg pfpdipehww lslfaklatt lgagdhlwil aaqmaehqvt kmggtdvdev
      121 tfvrymsgrm kfrltlrcsc crtlsgadvp pag
//
LOCUS       XP_077523758             192 aa            linear   INV 25-NOV-2025
DEFINITION  chitinase-3-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077523758
VERSION     XP_077523758.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667632.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 16% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..192
                     /product="chitinase-3-like protein 1"
                     /calculated_mol_wt=21448
     Region          <19..150
                     /region_name="GH18_chitinase-like"
                     /note="The GH18 (glycosyl hydrolase, family 18) type II
                     chitinases hydrolyze chitin, an abundant polymer of
                     beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a
                     major component of the cell wall of fungi and the
                     exoskeleton of arthropods. Chitinases have...; cl10447"
                     /db_xref="CDD:471972"
     CDS             1..192
                     /gene="LOC144134771"
                     /coded_by="XM_077667632.1:1..579"
                     /db_xref="GeneID:144134771"
ORIGIN      
        1 mhdaqvease aflgelrsrk rvalelslfg yayrlrntgs yelrasvrgp apprpfider
       61 gllvyfeicl efssdlsvpl ynkeadckia vnrdkgqwig fdnkatilnn vlfakrmrmg
      121 siavmtvdmd dyngncgqrn vllgqlhnal neldrpikrl sanlslsesa sssttapavv
      181 aqwnvqerym hc
//
LOCUS       XP_077523835            1482 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134870 [Amblyomma americanum].
ACCESSION   XP_077523835
VERSION     XP_077523835.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667709.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1482
                     /product="uncharacterized protein LOC144134870"
                     /calculated_mol_wt=167415
     Region          518..1078
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1482
                     /gene="LOC144134870"
                     /coded_by="XM_077667709.1:336..4784"
                     /db_xref="GeneID:144134870"
ORIGIN      
        1 meppakpflp gpmapttafc ardredncre malrvhclds gwgtptasql rrllglqcav
       61 gqvvagdcge vavyesptwt anvtlqlers pkdsfmhivl kwcdqcqnas nngaaagksg
      121 ppewwlasee leescdpyst qiatehkdvp vnafalgcfl gyntfvahfd stytmpppps
      181 plssakssss lsgksmsvkr gkkgrafkss spsdgtsssa ktscpnselt ssegfkvtqc
      241 nahfehcsng lylyfvnvrk rsstlfrllv rysticriil rktsptstiv ylqllhppll
      301 ykvcagnsat sqcnregdyl pqfdlsdttp wdravefgeg sfkcgsdvlg rsrmmrltls
      361 gprhwaplcn lilrtrknaq vfyapvrelh arelkavarp svqdfgvvya llsvwsegfq
      421 vtdelamdaq scretllels araeecpral eealfeiyfs lsqgkvfwfr salehlyfry
      481 wahrqridvf tgsviaqelp shmvkvrkvi ltptrrvymp pqlvcksrll qhcdpdyalr
      541 ivvrdddckl isfslgackd dflsavikpp lssglkiggr rfmflgcsts qlrshgvwfy
      601 ahdnqgrtae qirdgigdls tivsvpkfma rlgqafsqsl glltvpsqnt svepdirhaa
      661 pgrssdgshr eyifsdgigr ishgllrkvh haleleegee pcavqirygg ckgmllldpt
      721 lsgcrivfre smrkfhsdhs dlyvlktskp rvlylnrpmv tileqsgika evflmlqnki
      781 ldsfidsmmd pheaahvlrs ycawrlpyke lagvgidltv epffralvra vnkkvlkelr
      841 tkarilvppn ygrtmfgvld etgtleygqv fvqysndmlr ykvndpatil egdvivtknp
      901 cmnpgdirkl eavnvpqlhh vrdcivfpqk gerphpdema gsdldgdeys vlwyedlifn
      961 nncnpmhyhs dppkerkasi giqdmvdffc qyikgdkigl ianahlvwad ildsginshr
     1021 crelarkcav nldfakcgdl kgfqnsekpp mypdfmekld tkntycsrkv lgqlyrnckk
     1081 velsseclev veeslpdprl llegreqflk eatsaykrya kkirallksy rieteseafs
     1141 gavsklskym kendptdmsm vlesqvehvv rrtreeffse qleeahenlk asawyqvtye
     1201 lqsseggiqs fpwvvcdvlm rivvntsscl pvpasrnsfc qrlgalllgl phpgggdqdg
     1261 tqhgdtqvlt nllrlmydwi dssreflfvk nteelgvyks imreacfkvs rsisrdmpph
     1321 klvilclrfa cawclkifqg gsdgeviske crhryrlghl alitlnrlsm sgnlaylrra
     1381 pegcpsteli riyinredee ffeilrryed iikkimmdws gvediqcdlk tdrmdewflq
     1441 lmvtgsrwal erlkeivvyp sfrevlllaf ereknaiggt le
//
LOCUS       XP_077523985             192 aa            linear   INV 25-NOV-2025
DEFINITION  chitinase-3-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077523985
VERSION     XP_077523985.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667859.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..192
                     /product="chitinase-3-like protein 1"
                     /calculated_mol_wt=21417
     Region          <19..150
                     /region_name="GH18_chitinase-like"
                     /note="The GH18 (glycosyl hydrolase, family 18) type II
                     chitinases hydrolyze chitin, an abundant polymer of
                     beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a
                     major component of the cell wall of fungi and the
                     exoskeleton of arthropods. Chitinases have...; cl10447"
                     /db_xref="CDD:471972"
     CDS             1..192
                     /gene="LOC144135070"
                     /coded_by="XM_077667859.1:1..579"
                     /db_xref="GeneID:144135070"
ORIGIN      
        1 mhdaqvease aflgelrsrk rvalelslfg yayrlrntgs yelrasvrgp apprpfider
       61 gllvyfeicl efssdlsvpl ynkeadckia inrdkgqwig ydnnatilnn vlfakrmrmg
      121 siavvtvdmd dyngncgqrn vllgqlhnal neldrpikrl sanlspsesa sssttapaav
      181 aqwneqerym hc
//
LOCUS       XP_077524069             413 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135172 [Amblyomma americanum].
ACCESSION   XP_077524069
VERSION     XP_077524069.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667943.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 27% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..413
                     /product="uncharacterized protein LOC144135172"
                     /calculated_mol_wt=45334
     CDS             1..413
                     /gene="LOC144135172"
                     /coded_by="XM_077667943.1:1..1242"
                     /db_xref="GeneID:144135172"
ORIGIN      
        1 mapttafcar dredncrema lrvhcldsgw gtptasqlrr llglqcavgq vvagdcgeva
       61 vyesptwtan vtlqlerspr dsfmhivlkw cdqcqnasnn gaaagksgpp ewwlaseele
      121 efcdpystqi atehkdvpvn afalgcflgy ntfvahfdst ytmppppspl ssakssssls
      181 gksmsvkrgk kgravksssp sdgtsssakt pcpnseltss egfkvlgdqc nahfehcsng
      241 lylyfvdvrk rsstlfrllv rysticriil rktsptstiv dlqllhppll ykvcagnsat
      301 sqcnregdyl pqfdlsdttp wdravefgeg pfkcgadvlg rsrmmrltls gprhwaplcn
      361 lilrtrknaq vfyapvrelh arefkavarp svqdfgvvya llsvwsesfq vgi
//
LOCUS       XP_077524217             179 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135392 [Amblyomma americanum].
ACCESSION   XP_077524217
VERSION     XP_077524217.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668091.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..179
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..179
                     /product="uncharacterized protein LOC144135392"
                     /calculated_mol_wt=19283
     CDS             1..179
                     /gene="LOC144135392"
                     /coded_by="XM_077668091.1:1..540"
                     /db_xref="GeneID:144135392"
ORIGIN      
        1 mcsaivlsll spvqgireva vslaqaalns shkgcsrphl lfkeidqhaa evaadteell
       61 elivtsscgl kkqntllvll nhpqvpvaml ngvlqlvthv dskvtsclql galytleare
      121 cndsptsfvs fhsedfdhhw qpcwtasswi kasgstmsst lvvdkncwrv pelfsgaat
//
LOCUS       XP_077524333             192 aa            linear   INV 25-NOV-2025
DEFINITION  chitinase-3-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077524333
VERSION     XP_077524333.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668207.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..192
                     /product="chitinase-3-like protein 1"
                     /calculated_mol_wt=21350
     Region          <19..150
                     /region_name="GH18_chitinase-like"
                     /note="The GH18 (glycosyl hydrolase, family 18) type II
                     chitinases hydrolyze chitin, an abundant polymer of
                     beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a
                     major component of the cell wall of fungi and the
                     exoskeleton of arthropods. Chitinases have...; cl10447"
                     /db_xref="CDD:471972"
     CDS             1..192
                     /gene="LOC144135624"
                     /coded_by="XM_077668207.1:1..579"
                     /db_xref="GeneID:144135624"
ORIGIN      
        1 mhdaqvease aflgelrsrk rvalelslfg yayrlrntgs yelrasvrgp apprpfider
       61 gllvyfeicl efssdlsvpl ynkeadckia vnrdkgqwig ydnnatilnn vlfakrmrmg
      121 siavvtvdmd dyngncgqrn vllgqlhnal neldrpikrl sanlspsesa sssttapaav
      181 aqwneqecym hc
//
LOCUS       XP_077524337            1470 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135783 [Amblyomma americanum].
ACCESSION   XP_077524337
VERSION     XP_077524337.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668211.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1470
                     /product="uncharacterized protein LOC144135783"
                     /calculated_mol_wt=166246
     Region          506..1066
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1470
                     /gene="LOC144135783"
                     /coded_by="XM_077668211.1:1..4413"
                     /db_xref="GeneID:144135783"
ORIGIN      
        1 mapttafcar dredncrema lrvhcldsgw gtptasqlrr llglqcavgq vvagdcgeva
       61 vyesptwtan vtlqlerspr dsfmhivlkw cdqcqnasnn gaaagksgpp ewwlasaele
      121 vfcdpystqi atehkdvpvn afalgcflgy ntfvahfdst ytmppppspl ssakssssls
      181 gksmsvkrgk kgravksssp sdgtsssakt pcpnseltss egfkvtqcna hfehcyngly
      241 lyfvdvrkrs stlfrllvry sticriilrk tsptstivyl qllhppllyk vcagnsatsq
      301 cnregdylpq fdlsdttpwd ravefgegpf kcgsdvlgrs rmmrltlsgp rhwaplcnli
      361 lrtrknaqvf yapvrellar elkavarpsv qdfgvvyall svwsesfqvt delamdaqsc
      421 retllelsar aeecpralee alfeiyfsls qgkvfwfrsa lehlyfryra hrqridvftg
      481 sviaqelpsh mvkvrkvilt ptrrvymppq lvcksrllqh cdpdyalriv vrdddcklis
      541 fslgackddf lsavikppls sglkiggrrf mflgcstsql rshgvwfyah dnqgrtpeqi
      601 rdgigdlsti vsvpkfmarl gqafsqslgl ltvpsqntsv epdirhaapg rssdgshrey
      661 ifsdgigris hgllrkvhha leleegeepc avqiryggck gmllldptls gcrivfresm
      721 rkfhsdhsdl yvlktskprv lylnrpmiti leqsgikaev flmlqnkild sfidsmmdph
      781 eaahvlryyc alrlpykela gvgidltvep ffralvcavn kkvlkelrtk arilvppnyg
      841 rtmfgvldet gtleygqvfv qysndmlryk vndpatileg dvivtknpcm npgdirklea
      901 vnvpqlhhvr dcivfpqkge rphpdemags dldgdeysvl wyedlifnnn cnpmhyhsdp
      961 pkerkasigv qdmvdffcqy ikgdkiglia nahlvwadil dsginshrcr elarkcavnl
     1021 dfakcgdlkg fqnsekppmy pdfmekldtk ntycsrkvlg qlyrnckkve lsteclevve
     1081 eslpdprlll egreqflkea tsaykryakk irallksyri eteseafsga vsklskymke
     1141 ndptdmamvl esqvehvvrr treeffseql deaheklkas awyqvtyelq sseggiqsfp
     1201 wvvynvlmri vvntssclpv pasrnsfcqr lgalllgllh pgggdqdgtq hgdtqvltnl
     1261 lrlmydwids sreflfvknt eelgvyksim reacfkvsrs isrdmpphkl vilclrfaca
     1321 wclkifqggs dgeviskecr rryrlghlal itlnrlsmsg nlaylrrape gcpsteliri
     1381 yinredeeff eilrryedii kkimmdwsgv ediqcdlktd rmdewflqlm vtgsrwaler
     1441 lkeivvypsf revlllafer eknaiggtle
//
LOCUS       XP_077524350             192 aa            linear   INV 25-NOV-2025
DEFINITION  chitinase-3-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077524350
VERSION     XP_077524350.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668224.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..192
                     /product="chitinase-3-like protein 1"
                     /calculated_mol_wt=21385
     Region          <19..150
                     /region_name="GH18_chitinase-like"
                     /note="The GH18 (glycosyl hydrolase, family 18) type II
                     chitinases hydrolyze chitin, an abundant polymer of
                     beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a
                     major component of the cell wall of fungi and the
                     exoskeleton of arthropods. Chitinases have...; cl10447"
                     /db_xref="CDD:471972"
     CDS             1..192
                     /gene="LOC144136106"
                     /coded_by="XM_077668224.1:1..579"
                     /db_xref="GeneID:144136106"
ORIGIN      
        1 mhdaqvease aflgelrsrk rvalelslfg yayrlrntgs yelrasvrgp apprpfider
       61 gllvyfeicl efssdlsvpl ynkeadckia vnrdkgqwig ydnnatilnn vlfakrmrmg
      121 siavvtvdmd dyngncgqrn vllgqlhnal neldrpikrl sanlspsesa sssttapaav
      181 aqwneqeryi hc
//
LOCUS       XP_077524469             215 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136204 [Amblyomma americanum].
ACCESSION   XP_077524469
VERSION     XP_077524469.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668343.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 20% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..215
                     /product="uncharacterized protein LOC144136204"
                     /calculated_mol_wt=24412
     Region          <7..201
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..215
                     /gene="LOC144136204"
                     /coded_by="XM_077668343.1:1..648"
                     /db_xref="GeneID:144136204"
ORIGIN      
        1 mlrykvndpa tilegdvivt knpcmnpgdi rkleavnvpq lhhvrdcivf pqkgerphpd
       61 emagsdldgd eysvlwyedl ifnnncnpmh yhsdppkerk asigvqdmvd ffcqyikgdk
      121 iglianahlv wadildsgin shrcrelark cavnldfakc gdlkgfqnse qppmypdfme
      181 kldtkntycs rkvlgqlyrn ckkvqfitss tmtep
//
LOCUS       XP_077524607             121 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136247 [Amblyomma americanum].
ACCESSION   XP_077524607
VERSION     XP_077524607.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668481.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 23% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..121
                     /product="uncharacterized protein LOC144136247"
                     /calculated_mol_wt=13138
     CDS             1..121
                     /gene="LOC144136247"
                     /coded_by="XM_077668481.1:1..366"
                     /db_xref="GeneID:144136247"
ORIGIN      
        1 mcsaivlsll spvqgirela vslaqaalds shkgcsrphl lfkeidqhaa evaadteell
       61 elivtwshgl kkqntllvll nhpqvpvamq ngilqlvthv dsktaqaata arrsnfrrde
      121 w
//
LOCUS       XP_077525221             244 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136590 [Amblyomma americanum].
ACCESSION   XP_077525221
VERSION     XP_077525221.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077669095.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..244
                     /product="uncharacterized protein LOC144136590"
                     /calculated_mol_wt=26192
     Region          <12..191
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(54..59,107..108,154,156..157)
                     /site_type="active"
                     /db_xref="CDD:238827"
     Site            order(54..59,107,156)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238827"
     Site            108
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238827"
     CDS             1..244
                     /gene="LOC144136590"
                     /coded_by="XM_077669095.1:1..735"
                     /db_xref="GeneID:144136590"
ORIGIN      
        1 maegaqilge lqngfrpnhr mednlfvvts sieiaakekr elvlgfsgys pgiplsgtqs
       61 tvggpgdtgy lgatttlvea lytetqavih wrasttrpiy vyiglrqgcp mppiqfmlft
      121 srlerrlvec gvgfnltcmk dgttrthrlp vllyaddivl lvesvgdlqs lvdmcssegd
      181 dlglrfsaak slsaatpppl lsdhleqltr tlvttlqavl qlfpvasqpp qcgagpaqqp
      241 nhhg
//
LOCUS       XP_077525342             257 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136657 [Amblyomma americanum].
ACCESSION   XP_077525342
VERSION     XP_077525342.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077669216.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..257
                     /product="uncharacterized protein LOC144136657"
                     /calculated_mol_wt=27644
     Region          <12..196
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            108
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238827"
     CDS             1..257
                     /gene="LOC144136657"
                     /coded_by="XM_077669216.1:1..774"
                     /db_xref="GeneID:144136657"
ORIGIN      
        1 maegaqilge lqngfrpnhr lednlfvvts sieiaakekr elvlgfsgys pgiplsgtqs
       61 tvggpgdtgy lgatttlvea lytetqavih wrasttrpiy lyiglrqgcp mppiqfklft
      121 srlerrlvec gvgfnltcmk dgttrthrlp vllyaddivl lvesvgdlqs lvdmcssegd
      181 dlglrfnaak slsvrfqpea sedsgqaatp ppllsdhleq ltrtlvttlq avlqlfpvas
      241 qppqcgagpa qqpnhhg
//
LOCUS       XP_077525468             364 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136729 [Amblyomma americanum].
ACCESSION   XP_077525468
VERSION     XP_077525468.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077669342.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..364
                     /product="uncharacterized protein LOC144136729"
                     /calculated_mol_wt=41067
     CDS             1..364
                     /gene="LOC144136729"
                     /coded_by="XM_077669342.1:1..1095"
                     /db_xref="GeneID:144136729"
ORIGIN      
        1 msdlpmdght llrtprkpqe qcpvtplapg hycyfaladg llrllrlven ppaliplsfn
       61 idglplskss kmqlwpiqcl ahdcgdetpf lvgafagksk pdssveflrp fvselrtlls
      121 eglifngrvl hvimayaive flhheqvevv psswiddrqc awpdhakgdr ivslvrkaap
      181 pdsswkrfdi alkglfgayk lgrrkldksq ytselssdge pphkrtrrpp qawsedevsd
      241 tpttkvpyre nvpsvphdfp qgdfqelvri gdkdlfdkgh egearlsdrr dsvdedfkqh
      301 virllnilrf tqqqhgdmln klcevlpsqa iitkpllise pftsvddllk fnekldkenc
      361 tses
//
LOCUS       XP_077482967             407 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093429 [Amblyomma americanum].
ACCESSION   XP_077482967
VERSION     XP_077482967.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077626841.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..407
                     /product="uncharacterized protein LOC144093429"
                     /calculated_mol_wt=43169
     CDS             1..407
                     /gene="LOC144093429"
                     /coded_by="XM_077626841.1:50..1273"
                     /db_xref="GeneID:144093429"
ORIGIN      
        1 mfklsrirgf vkrkliqqal vpiiwaarrl qrqqqvverr pnpgleeeqv edgdfegkir
       61 lqgrlesnil gpsfkyylkk svcvrahqql raqvggpqsv riiwrpvgpr tpprpplplp
      121 grkknfavqs kidsglqrkr aaaaacefqr atkgkgnges eeeassssnp dgsrgnrarq
      181 grragaarga rpgreapaes gaepaggled slkgaaapgg srglparrpe grrgrlrggr
      241 iargqlhadi atsvgnedev aaptsslarr arqagqaarg nqafeeeass ssnpdgsrgn
      301 rarqgrraga argarpgrea paetraepag gaidllkgaa apdasrglpa rraegrrgrp
      361 rgrhaqpgat igdgggdkga teaqsgeghm akpgrgrqrr rprngsr
//
LOCUS       XP_077483094             106 aa            linear   INV 25-NOV-2025
DEFINITION  histone-lysine N-methyltransferase set-17-like [Amblyomma
            americanum].
ACCESSION   XP_077483094
VERSION     XP_077483094.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077626968.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..106
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..106
                     /product="histone-lysine N-methyltransferase set-17-like"
                     /calculated_mol_wt=11783
     Region          6..105
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(12..13,68..72)
                     /site_type="other"
                     /note="SAM binding site [polypeptide binding]"
                     /db_xref="CDD:380914"
     CDS             1..106
                     /gene="LOC144093488"
                     /coded_by="XM_077626968.1:1..321"
                     /db_xref="GeneID:144093488"
ORIGIN      
        1 masgkstvkg apvgvftlka lpkglhfgpy hgvkvdsien ggctwpvrrc edffvvdgrp
       61 qernhwmndv nyspskrrqn lvallvngdi yyrtirnvgp eeelll
//
LOCUS       XP_077483218             279 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093530 [Amblyomma americanum].
ACCESSION   XP_077483218
VERSION     XP_077483218.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627092.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 48% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..279
                     /product="uncharacterized protein LOC144093530"
                     /calculated_mol_wt=30498
     CDS             1..279
                     /gene="LOC144093530"
                     /coded_by="XM_077627092.1:1..840"
                     /db_xref="GeneID:144093530"
ORIGIN      
        1 matppkrprm svaqglllie lleqhpplar aaselspeyp aarrgelwer iaaalnregp
       61 avksaelwgt sggrlsgrag hvvqlvgpeq aspphslspp rghrasvgsv pgtsglqreh
      121 rsaaddtnms wagasettpl elleeeqqhq haeiastqqp talstlpsfa sppsppvpgr
      181 pvwrrrqrde evadllretr rtadltearn rrdaefqarl lehlneaaad rrqltatvad
      241 laaavrdtre qsvrlheqlv vavsflllml hyqihppph
//
LOCUS       XP_077483577             192 aa            linear   INV 25-NOV-2025
DEFINITION  chitinase-3-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077483577
VERSION     XP_077483577.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627451.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..192
                     /product="chitinase-3-like protein 1"
                     /calculated_mol_wt=21304
     Region          <19..150
                     /region_name="GH18_chitinase-like"
                     /note="The GH18 (glycosyl hydrolase, family 18) type II
                     chitinases hydrolyze chitin, an abundant polymer of
                     beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a
                     major component of the cell wall of fungi and the
                     exoskeleton of arthropods. Chitinases have...; cl10447"
                     /db_xref="CDD:471972"
     CDS             1..192
                     /gene="LOC144093733"
                     /coded_by="XM_077627451.1:1..579"
                     /db_xref="GeneID:144093733"
ORIGIN      
        1 mhdaqvease aflgelrsrk rvalelslfg yayglrntgs yelrasvrgp apprpfider
       61 gllvyfeicl efssdlsvpl ynkeadckia vnrdkgqwig ydnkatilnn vlfakrmrmg
      121 siavvtvdmd dyngncgqrn vllgqlhnal neldrpikrl sanlslsesa sssttapaav
      181 aqwnvqerym hc
//
LOCUS       XP_077483684            1482 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093813 [Amblyomma americanum].
ACCESSION   XP_077483684
VERSION     XP_077483684.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627558.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1482
                     /product="uncharacterized protein LOC144093813"
                     /calculated_mol_wt=167282
     Region          518..1078
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1482
                     /gene="LOC144093813"
                     /coded_by="XM_077627558.1:336..4784"
                     /db_xref="GeneID:144093813"
ORIGIN      
        1 meppakpflp gpmapttafc ardredncre malrvhclds gwgtptasql rrllglqcav
       61 gqvvagdcge vavyesptwt anvtlqlers pkdsfmhivl kwcdqcqnas nngaaagksg
      121 ppewwlasee leescdpyst qiaaehkdvp vnafalgcfl gyntfvahfd stytmpppps
      181 plssakssss lsgksmsvkr gkkgravkss spsdgtsssa ktscpnselt ssegfkvtqc
      241 nahfehcsng lylyfvdvrk rsstlfrllv rysticriil rktsptstiv ylqllhppll
      301 ykvcagnsat sqcnregdyl pqfdlsdttp wdravefgeg pfkcgsdvlg rsrmmrltls
      361 gprhwaplcn lilrtrknaq vfyapvrell arelkavarp svqdfgvvya llsvwsesfq
      421 vtdelamdaq scretllels araeecpral eealfeiyfs lsqgkvfwfr salehlyfry
      481 rahrqridvf tgsviaqelp shmvkvrkvi ltptrrvymp pqllcksrll qhcdpdyalr
      541 ivvrdddckl isfslgackd dflsavikpp lssglkiggr rfmflgcsts qlrshgvwfy
      601 ahdnqgrtae qirdgigdls tivsvpkfma rlgqafsqsl glltvpsqnt svepdirhaa
      661 pgrssdgshr eyifsdgigr ishgllrkvh haleleeaee pcavqirygg ckgmllldpt
      721 lsgcrivfre smrkfhsdhs dlyvlktskp rvlylnrpmi tileqsgika evflmlqnki
      781 ldsfidsmmd pheaahvlrs ycalrlpyke lagvgidltv epffralvra vnkkvlkelr
      841 tkarilvppn ygrtmfgvld etgtleygqv fvqysndmlq ykvndpatil egdvivtknp
      901 cmnpgdirkl eavnvpqlhh vrdcivfpqk gerphpdema gsdldgdeys vlwyedlifn
      961 nncnpmhyhs dppkerkasi gvqdmveffc qyikgdkigl ianahlvwad ildsginshr
     1021 crelarkcav nldfakcgdl kgfqnsekpp mypdfmekld tkntycsrkv lgqlyrnckk
     1081 velsteclev veeslpdprl llegreqflk eatsaykrya kkirallksy rieteseafs
     1141 gavsklskym kendptdmam vlesqvehvv rrtreeffse qldeaheklk asawyqvtye
     1201 lqsseggiqs fpwvvcdvlm rivvntsscl pvpasrnsfc qrlgalllgl phpgggdqdg
     1261 tqhgdtqvlt nllrlmydwi dssreflfvk nteelgvyks imreacfkvs rsisrdmpph
     1321 klvilclrfa cawclkifqg gsdgeviske crrryrlghl alitlnrlsm sgnlaylrra
     1381 pegcpsteli riyinredee ffeilrryed iikkimmdws gvediqcdlk tdrmdewflq
     1441 lmvtgsrwal erlkeivvyp sfrevlllaf ereknaiggt le
//
LOCUS       XP_077483916            1482 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093966 [Amblyomma americanum].
ACCESSION   XP_077483916
VERSION     XP_077483916.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627790.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1482
                     /product="uncharacterized protein LOC144093966"
                     /calculated_mol_wt=167325
     Region          518..1078
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1482
                     /gene="LOC144093966"
                     /coded_by="XM_077627790.1:336..4784"
                     /db_xref="GeneID:144093966"
ORIGIN      
        1 meppakpflp gpmapttafc ardredncre malrvhclds gwgtptasql rrllglqcav
       61 gqvvagdcge vavyesptwt anvtlqlers pkdsfmhivl kwcdqcqnas nngaaagkss
      121 ppewwlasee leescdpyst qiatehkdvp vnafalgcfl gyntfvahfd stntmpppps
      181 plssakssss lsgksmsvkr gkkgravkss spsdgtsssa ktscpnselt ssegfkvtqc
      241 nahfehcsng lylyfvdvrk rsstlfrllv rysticriil rktsptstiv ylqllhppll
      301 ykvcagnsat sqcnregdyl pqfdlsdttp wdravefgeg pfkcgsdvlg rsrmmrltls
      361 gprhwaplcn lilrtrknaq vfyapvrell arelkavarp svqdfgvvya llsvwsesfq
      421 vtdelamdaq scretllels araeecpral eealfeiyfs lsqgkvfwfr salehlyfry
      481 rahrqridvf tgsviaqelp shmvkvrkvi ltptrrvymp pqlvcksrll qhcdpdyalr
      541 ivvrdddckl isfslgackd dflsavikpp lssglkiggr rfmflgcsts qlrshgvwfy
      601 ahdnqgrtae qirdgigdls tivsvpkfma rlgqafsqsl glltvpsqnt svepdirhaa
      661 pgrssdgshr eyifsdgigr ishgllrkvh haleleegee pcavqirygg ckgmllldpt
      721 lsgcrivfre smrkfhsdhs dlyvlktskp rvlylnrpmi tileqsgika evflmlqnki
      781 ldsfidsmmd pheaahvlrs ycalrlpyke lagvgidltf epffralvra vnkkvlkelr
      841 tkarilvppn ygrtmfgvld etgtleygqv fvqysndilq ykvndpatil egdvivtknp
      901 cmnpgdirkl eavnvpqlhh vrdcivfpqk gewphpdema gsdldgdeys vlwyedlifn
      961 nncnpmhyhs dppkerkasi gvqdmveffc qyikgdkigl ianahlvwad ildsginshr
     1021 crelarkcav nldfakcgdl kgfqnsekpp mypdfmekld tkntycsrkv lgqlyrnckk
     1081 velsteclev veeslpdprl llegreqflk eatsaykrya kkirallksy rieteseafs
     1141 gavsklskym kendptdmam vlesqvehvv rrtreeffse qldeaheklk asawyqvtye
     1201 lqsseggiqs fpwvvcdvlm rivvntsscl pvpasrnsfc qrlgalllgl phpgggdqdg
     1261 tqhgdtqvlt nllrlmydwi dssreflfvk nteelgvyks imreacfkvs rsisrdmpph
     1321 klvilclrfa cawclkifqg gsdgeviske crrryrlghl alitlnrlsm sgnlaylrra
     1381 pegcpsteli riyinredee ffeilrryed iikkimmdws gvediqcdlk tdrmdewflq
     1441 lmvtgsrwal erlkeivvyp sfrevlllaf ereknaiggt le
//
LOCUS       XP_077484030             333 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094023 [Amblyomma americanum].
ACCESSION   XP_077484030
VERSION     XP_077484030.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627904.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..333
                     /product="uncharacterized protein LOC144094023"
                     /calculated_mol_wt=36279
     CDS             1..333
                     /gene="LOC144094023"
                     /coded_by="XM_077627904.1:324..1325"
                     /db_xref="GeneID:144094023"
ORIGIN      
        1 meppakpflp gpmapttafc ardredncre malrvhclds gwgtptasql rrllglqcav
       61 gqvvagdcge vavyesptwt anvtlqlers pkdsfmhivl kwcdqcqnas nngaaagksg
      121 ppewwlasee leefcdpyst qiatehkdvp vnafalgcfl gyntfvahfd stytmpppps
      181 plssakssss lsgksmsvkr gkkgravkss spsdgtsssa ktscpnselt ssegfkvtqc
      241 nahfehcsng lylyfvdvrk rsstlfrllv rysticriil rktsptstiv ylqllhppll
      301 ykvcagnsat sqcnregdyl pqfplvvefy ita
//
LOCUS       XP_077484229             259 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094159 [Amblyomma americanum].
ACCESSION   XP_077484229
VERSION     XP_077484229.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628103.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 14% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..259
                     /product="uncharacterized protein LOC144094159"
                     /calculated_mol_wt=29265
     CDS             1..259
                     /gene="LOC144094159"
                     /coded_by="XM_077628103.1:1..780"
                     /db_xref="GeneID:144094159"
ORIGIN      
        1 makqsfgpdg halwhmgpls iiwfltclvt qcnahfehcs nglylhfvdv rkrsstrfrl
       61 lvrysticri ilrktsptst ivylqllhpp llykvcagns atsqcnregd ylpqfdlsdt
      121 tpwdravefg egpfkcgadv lgrsrmmrlt lsgprhwapl cnlilrtrkn aqvfyapvre
      181 lqarelkava rpsvqdfgvv yallsvwset fqvtdelamd aqscretlle lsaraeecpr
      241 aleealfeiy fslsqgkai
//
LOCUS       XP_077484330             208 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094233 [Amblyomma americanum].
ACCESSION   XP_077484330
VERSION     XP_077484330.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628204.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 36% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..208
                     /product="uncharacterized protein LOC144094233"
                     /calculated_mol_wt=22014
     CDS             1..208
                     /gene="LOC144094233"
                     /coded_by="XM_077628204.1:1..627"
                     /db_xref="GeneID:144094233"
ORIGIN      
        1 malsvhclds gwgtptasql rrllglqctv gqvvtgdcge vavyesptwt anvtlqlers
       61 pkdsfmhivl kwcdqcqnas nngaaagksg ppewwlasee leefcdpyst qiatehkdvp
      121 vnafalgcfl gyntfvahfd stntmpppps plssakssss lsgksmsvkr gkkgravkst
      181 spsdgtsssa ktscpnselt ssegfkvr
//
LOCUS       XP_077484449             564 aa            linear   INV 25-NOV-2025
DEFINITION  F-box only protein 41-like [Amblyomma americanum].
ACCESSION   XP_077484449
VERSION     XP_077484449.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628323.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 31% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..564
                     /product="F-box only protein 41-like"
                     /calculated_mol_wt=62693
     Region          242..282
                     /region_name="F-box"
                     /note="F-box domain; pfam00646"
                     /db_xref="CDD:425796"
     CDS             1..564
                     /gene="LOC144094330"
                     /coded_by="XM_077628323.1:1..1695"
                     /db_xref="GeneID:144094330"
ORIGIN      
        1 mtttvltsgc hcclpecsma ppvyrcpicq aasksfrsll hhvqwwhgfc hdsppgpehr
       61 gesngvpnyd lvrkrdaylr eqgklrakid dffhrteasm kldrealsyq yaadtspphi
      121 yprerppaat vyqefcpltv pkegqhpvht vtdssnvgsr dkveaavqaa eirrhqsnel
      181 fnfpkdaycp akdmtgsyds shgdeviera tqtkprprrl agkprvkfal nardtapsks
      241 adlaenvlet viqflstedl licrrvcraw kriverphhw lhidlnhtsv saelfsemaa
      301 wcsateslsl qaleattdan krvpgahleh glekllmacs ktlkvltvvg cdnlltdrqv
      361 lgaenwyafi vqvlwlasac pfleavayvt ashvtlpeal wslgagcrri kalivppldp
      421 qkrqgyaghr vcqmlgkcwp qlqvacvggr sltlasvccv lrscvnlvsf eidrgpeide
      481 asaeamveag lqrierlyft htvishravv ilqgacprmk qtsiilhgrd fgcasalnnc
      541 vaslkvlqrt kpfdimlrlv ltde
//
LOCUS       XP_077484576             120 aa            linear   INV 25-NOV-2025
DEFINITION  putative nicotine oxidoreductase [Amblyomma americanum].
ACCESSION   XP_077484576
VERSION     XP_077484576.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628450.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..120
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..120
                     /product="putative nicotine oxidoreductase"
                     /calculated_mol_wt=13336
     Region          <3..>120
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(37..42,97)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238185"
     Site            98
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     CDS             1..120
                     /gene="LOC144094505"
                     /coded_by="XM_077628450.1:1..363"
                     /db_xref="GeneID:144094505"
ORIGIN      
        1 mlgfranlst qdvllqlkee vletmtkqge nvfmalnikg pfdnvghadi merlnntkcg
       61 rrfydyvkdf ftkrtatvgl velrsdvftt pskgtpqgsv ispilinvam iglarrlsri
//
LOCUS       XP_077484682             147 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094603 [Amblyomma americanum].
ACCESSION   XP_077484682
VERSION     XP_077484682.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628556.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 25% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..147
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..147
                     /product="uncharacterized protein LOC144094603"
                     /calculated_mol_wt=16486
     CDS             1..147
                     /gene="LOC144094603"
                     /coded_by="XM_077628556.1:161..604"
                     /db_xref="GeneID:144094603"
ORIGIN      
        1 mpepivpppq vprrtinaaa iefsrllhet dasvfdspes alkifqrgde lfrgtnddvp
       61 pltapcngdg ertrcwalny qrmlnrmlne rgvelfemqe sallcqtisc tdvpqasvhs
      121 krhrervyyg asppqevlep paikris
//
LOCUS       XP_077484777             641 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094705 [Amblyomma americanum].
ACCESSION   XP_077484777
VERSION     XP_077484777.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628651.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..641
                     /product="uncharacterized protein LOC144094705"
                     /calculated_mol_wt=71394
     Region          423..>641
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..641
                     /gene="LOC144094705"
                     /coded_by="XM_077628651.1:1..1926"
                     /db_xref="GeneID:144094705"
ORIGIN      
        1 mhivlkwcdq cqnasnngaa agksgppeww laseeleefc dpystqiate hkdvpvnafa
       61 lgcflgyntf vahfdstntm ppppsplssa ksssslsgks msvkrgkkgr avkstspsdg
      121 tsssaktscp nseltssegf kvtqcnahfe hcsnglylyf vdvrkrsstr frllvrysti
      181 criilrktsp tstivylqll hppllykvca gnsatsqcnr egdylpqfdl sdttpwdrav
      241 efgegpfkcg advlgrsrmm rltlsgprhw aplcnlilrt rknaqvfyap vrelqardlk
      301 avarpsvqdf gvvyallsvw sesfqvtdel amdaqscret llelsaraee cpraleealf
      361 eiyfslsqgk vfwfrsaleh lyfrfrahrq ridvftgsvi aqelpshmvk vrkviltptr
      421 rvymppqlvc ksrllqhcdp dyalrivvrd ddcklisfsl gackddflsa vikpplssgl
      481 kiggrrfmfl gcstsqlrsh glwfyahdnq grtaeqirdg igdlstivsv pkfmarlgqa
      541 fsqslglltv psqntsvepd irhaapgrss dgshreyifs dgigrishgl lrkvhhalel
      601 eegeepcavq iryggckgml lldptlsgcr ivfrksmrkf h
//
LOCUS       XP_077484854             132 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094811 [Amblyomma americanum].
ACCESSION   XP_077484854
VERSION     XP_077484854.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628728.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 25% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..132
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..132
                     /product="uncharacterized protein LOC144094811"
                     /calculated_mol_wt=14406
     CDS             1..132
                     /gene="LOC144094811"
                     /coded_by="XM_077628728.1:1..399"
                     /db_xref="GeneID:144094811"
ORIGIN      
        1 mrdesgagte ksrtasphps igmplkknir scvnlvsfei drgreideas aeamvkaglq
       61 rvehlyftht vishravvil qgacprmket siilhgrdfg casalndcva slkmlqrtkp
      121 fdtvlklvls de
//
LOCUS       XP_077484937             126 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094911 [Amblyomma americanum].
ACCESSION   XP_077484937
VERSION     XP_077484937.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628811.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 30% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..126
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..126
                     /product="uncharacterized protein LOC144094911"
                     /calculated_mol_wt=14464
     CDS             1..126
                     /gene="LOC144094911"
                     /coded_by="XM_077628811.1:11..391"
                     /db_xref="GeneID:144094911"
ORIGIN      
        1 mydwidssre flfvkdaeel glyknimrea cvkvsrslsr dmpphklvil clrfacawcl
       61 kifqggsdge viskecrrry rlghlalitl nrfsisgnlt ylkrapegca steliryish
      121 lyskpe
//
LOCUS       XP_077484986             351 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095014 [Amblyomma americanum].
ACCESSION   XP_077484986
VERSION     XP_077484986.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628860.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 12% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..351
                     /product="uncharacterized protein LOC144095014"
                     /calculated_mol_wt=40224
     CDS             1..351
                     /gene="LOC144095014"
                     /coded_by="XM_077628860.1:1..1056"
                     /db_xref="GeneID:144095014"
ORIGIN      
        1 mrppnplcff vqvssvtsly akksndvcll lfprpgvllc vfsecfdivn qllalsgdve
       61 lnpgrntrhq vqdslpdvqs kqledmfail etlntrtakm qldqkafiks iddlkksqlk
      121 ledlsemnkr ltnverktvt idetekdvhp fqqlvdqlss ensqlrarls eledrqrrdn
      181 llfygipdaq seswaqseek lvkvlsscld ipsseilidr ahrlggftse kcrpiiakfy
      241 sikvkqrill ncsklkdeki tlsedfsfat lharnklvef gksqpltfkl rfnklyiknk
      301 cysynhsdds vheipstfpl ptvrsedptn dtlratdrtc yqlalregqw n
//
LOCUS       XP_077485077             463 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095119 [Amblyomma americanum].
ACCESSION   XP_077485077
VERSION     XP_077485077.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628951.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..463
                     /product="uncharacterized protein LOC144095119"
                     /calculated_mol_wt=50894
     CDS             1..463
                     /gene="LOC144095119"
                     /coded_by="XM_077628951.1:1..1392"
                     /db_xref="GeneID:144095119"
ORIGIN      
        1 malsvhclds gwgtptasql rrllglqctv gqvvagdcge vavyesptwt anvtlqlers
       61 pkdsfmhivl kwcdqcqnas nngaaagksg ppewwlasee leefcdpyst qiatehkdvp
      121 vnafalgcfl gyntfvahfd stntmpppps plssakssss lsgksmsvkr gkkgravkss
      181 spsdgtsssa ktscpnselt ssegfkvtqc nahfehcsng lylyfvdvrk rsstrfrllv
      241 rysticriil rktsptstiv ylqllhppll ykvcagnsat sqcnregdyl pqfdlsdttp
      301 wdravefgeg pfkcgadvlg rsrmmrltls gprhwaplcn lilrtrknaq vfyapvrelq
      361 arelkavarp svqdfgvvya llsvwsesfq vtdelamdaq scretllels araeecpral
      421 eealfeiyfs lsqgknsvlk taaetvfler kldsshynsq ghv
//
LOCUS       XP_077485254             272 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095332 [Amblyomma americanum].
ACCESSION   XP_077485254
VERSION     XP_077485254.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629128.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..272
                     /product="uncharacterized protein LOC144095332"
                     /calculated_mol_wt=30291
     Region          <185..261
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     CDS             1..272
                     /gene="LOC144095332"
                     /coded_by="XM_077629128.1:406..1224"
                     /db_xref="GeneID:144095332"
ORIGIN      
        1 mpepivpppq vqcrttnaaa fefsrllhet dasvfdspes alkifqrgde lfrgtnddvp
       61 pltapcngdg ertrcwalny qrmlnrmlne rgvelfemhe sallcqtisc tdvpqgtdda
      121 miptvllwll rqhqcirsvi vnasimaphp lkkfwsllll selvksvelr dveensfsih
      181 rvfhehaerl tvlvlmgfnl svadathlaa lvarnkglkk lalvdlyida asmeeivasi
      241 eghdgldele ltalrrtadg ksiarlleah fa
//
LOCUS       XP_077485320             295 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095428 [Amblyomma americanum].
ACCESSION   XP_077485320
VERSION     XP_077485320.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629194.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..295
                     /product="uncharacterized protein LOC144095428"
                     /calculated_mol_wt=32332
     CDS             1..295
                     /gene="LOC144095428"
                     /coded_by="XM_077629194.1:147..1034"
                     /db_xref="GeneID:144095428"
ORIGIN      
        1 msrtpkaaag pnqlrdelrq qllttfaank resamgegtq avedscsaas gkplkkqplp
       61 vvsatscgch kyddlhvhvt vlcpcsrptt tdkatqtdma rtdeqqrstp rgvgisvdil
      121 htsnermfsq tcshelaasv pngtpvtprl qgvpnsadis vqafedegnv siedgskeew
      181 ypahqvfnga dtsrispadr rrsvhealar vrdtlfealr dtpramaats raailysrac
      241 fppcqprrlr hhplrsrspr cfgfyrrvrq rhsvrgkctl tsmdsfssss dvtpv
//
LOCUS       XP_077485366             393 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095515 [Amblyomma americanum].
ACCESSION   XP_077485366
VERSION     XP_077485366.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629240.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 46% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..393
                     /product="uncharacterized protein LOC144095515"
                     /calculated_mol_wt=43033
     CDS             1..393
                     /gene="LOC144095515"
                     /coded_by="XM_077629240.1:1..1182"
                     /db_xref="GeneID:144095515"
ORIGIN      
        1 malsvhclds gwgtptasql rrllglqctv gqvvagdcge vtvyesptwt anvtlqlers
       61 pkdsfmhivl kwcdqcqnas nngaaagksg ppewwlasee leefcdpyst qiatehkdvp
      121 vnafalgcfl gyntfvahfd stntmpppps plssakssss lsgksmsvkr gkkgravkst
      181 spsdgtsssa ktscpnselt ssegfkvtqc nahfehcsng lylyfvdvrk rsstrfrllv
      241 rysticriil rktsptstiv ylqllhppll ykvcagnsat sqcnregdyl pqfdlsdttp
      301 wdravefgdg pfkcgadvlg rsrmmrltls gprhwaplcn lilrtrknaq vfyapvrelq
      361 arelkavarp svqdfgvvya llsvwsesfq vgi
//
LOCUS       XP_077485422             131 aa            linear   INV 25-NOV-2025
DEFINITION  F-box only protein 41-like [Amblyomma americanum].
ACCESSION   XP_077485422
VERSION     XP_077485422.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629296.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 22% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..131
                     /product="F-box only protein 41-like"
                     /calculated_mol_wt=14302
     CDS             1..131
                     /gene="LOC144095598"
                     /coded_by="XM_077629296.1:405..800"
                     /db_xref="GeneID:144095598"
ORIGIN      
        1 mlgkcwpqlq vacvggrslt lasvccvlrs cvnlvsfeid rgreideasa eamveaglqr
       61 ierlyfthtv ishravvilq gacprmkqts iilhgrdfgc asalnncvas lkvlqrtkpf
      121 dimlrlvlad e
//
LOCUS       XP_077485484             171 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095684 [Amblyomma americanum].
ACCESSION   XP_077485484
VERSION     XP_077485484.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629358.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 40% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..171
                     /product="uncharacterized protein LOC144095684"
                     /calculated_mol_wt=19263
     CDS             1..171
                     /gene="LOC144095684"
                     /coded_by="XM_077629358.1:39..554"
                     /db_xref="GeneID:144095684"
ORIGIN      
        1 mihqefmeic kvdferkllg finghgqrlf silktsrtaq kifsevqkai adsegdakky
       61 cfavgvlqll phlvkeeafy lrgpdsypci vmndkphtat nvcatfenmk veaidvisgf
      121 atvmeiywvm nieyskknkn tfavlehfcg lkskplkpvt lraialleka n
//
LOCUS       XP_077485489             394 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095831 [Amblyomma americanum].
ACCESSION   XP_077485489
VERSION     XP_077485489.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629363.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 40% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..394
                     /product="uncharacterized protein LOC144095831"
                     /calculated_mol_wt=44488
     Region          <185..277
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     CDS             1..394
                     /gene="LOC144095831"
                     /coded_by="XM_077629363.1:246..1430"
                     /db_xref="GeneID:144095831"
ORIGIN      
        1 mpepivpppq vprrtinaas iefsrllhet dasvfdspes alkifqrgde lfrgtnddvp
       61 pltapcngdg ertrcwalny qrmlnrmlne rgvelfemqe sallcqtisc tdvpqgtdda
      121 miptvllwll rqhqcirsvi vnasimaphp lkkfwsllll selvksvelr dveensfsih
      181 rvfhehaerl tvlvlmgfnl svadathlaa lvarnkglkk lalvdlyida asmeeivasi
      241 dghdgldele ltalrrtadg ksiarlleah itklrlgvec dwepfldalv tnidlvelai
      301 vsrstlafsl hrlvravsis etlesleldl dtswaidddr iweelaaive rlyksearry
      361 kpphysegqh ydfevellsd iekylnlwnv slse
//
LOCUS       XP_077485498             197 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095992 [Amblyomma americanum].
ACCESSION   XP_077485498
VERSION     XP_077485498.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629372.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..197
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..197
                     /product="uncharacterized protein LOC144095992"
                     /calculated_mol_wt=21830
     CDS             1..197
                     /gene="LOC144095992"
                     /coded_by="XM_077629372.1:41..634"
                     /db_xref="GeneID:144095992"
ORIGIN      
        1 martdeqqrs tprgvgisvd ilhtsnermf sqtcshelaa sapngtpvtp rlqgvpnsad
       61 isvqafedeg nvsiedgske ewypahqvfn gadtsrispa drrrsvheal arvrdtlfea
      121 lrdtpravaa tsraailysr acfppcqprr lrhhplrsrs prcfgfyrrv rqrhsvrgkc
      181 pltsmdsfss ssdvtpv
//
LOCUS       XP_077485508             388 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096153 [Amblyomma americanum].
ACCESSION   XP_077485508
VERSION     XP_077485508.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629382.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 42% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..388
                     /product="uncharacterized protein LOC144096153"
                     /calculated_mol_wt=43475
     Region          225..265
                     /region_name="F-box"
                     /note="F-box domain; pfam00646"
                     /db_xref="CDD:425796"
     CDS             1..388
                     /gene="LOC144096153"
                     /coded_by="XM_077629382.1:1..1167"
                     /db_xref="GeneID:144096153"
ORIGIN      
        1 mappvyrcpi cqaasksfrs llhhvqwwrg fchdsppape hrgesnavpn ydlvrkrday
       61 lreqgklrak iddffqrtea rmkldreals yqmyaadtsp phiyprerpp aatvyqefcs
      121 ltvpkegqhp vhtvtdssnv gcrdkveaav qaaeilshqs nelfifpkda ycpgkdmtgs
      181 ygsshgdeai eratqtklrp rrlagkprvn falnardtap sktadlaenv letviqflst
      241 edllicrrvc rawkriverp qhwlhidlnh tsvsaelfse maalcsates lslqaleata
      301 dankrvpgah lehglekllm acsktlkvlt vvgcdnlltd rqvlgavnwy afivqlannp
      361 wlhpahkate sappnllwir npnrmsrg
//
LOCUS       XP_077485517             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096312 [Amblyomma americanum].
ACCESSION   XP_077485517
VERSION     XP_077485517.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629391.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 28% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144096312"
                     /calculated_mol_wt=14235
     CDS             1..127
                     /gene="LOC144096312"
                     /coded_by="XM_077629391.1:309..692"
                     /db_xref="GeneID:144096312"
ORIGIN      
        1 mpepivpppq vprrtinaaa iefsrllhet dasvfdspes alkifqrgde lfrgtnddvp
       61 pltapcngdg ertrcwalny qrmlnrmlne rgvelfemqe sallcqtisc tdvpqasvhs
      121 krhrecv
//
LOCUS       XP_077485523             112 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatase Herzog-like [Amblyomma americanum].
ACCESSION   XP_077485523
VERSION     XP_077485523.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629397.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..112
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..112
                     /product="phosphatase Herzog-like"
                     /calculated_mol_wt=12202
     Region          41..>111
                     /region_name="HAD_like"
                     /note="Haloacid Dehalogenase-like Hydrolases; cl21460"
                     /db_xref="CDD:473868"
     CDS             1..112
                     /gene="LOC144096473"
                     /coded_by="XM_077629397.1:93..431"
                     /db_xref="GeneID:144096473"
ORIGIN      
        1 matyssmdly sscptamfvg taptengkfl lppvryqdmh kicliidlde tlvhssikpi
       61 snadfvvpve tggtvhqvyv lkrpyvdefl qrvgdayecv lftaghakaa sc
//
LOCUS       XP_077485679             162 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096684 [Amblyomma americanum].
ACCESSION   XP_077485679
VERSION     XP_077485679.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629553.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..162
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..162
                     /product="uncharacterized protein LOC144096684"
                     /calculated_mol_wt=18229
     CDS             1..162
                     /gene="LOC144096684"
                     /coded_by="XM_077629553.1:290..778"
                     /db_xref="GeneID:144096684"
ORIGIN      
        1 mtgtedggrp slllvspmhv vtlsdrrswg vrlaqrlhai sqrwfthvll lvllaayaal
       61 galafryleg pyesrakagl qrvrrllvee lrrgcgaadd hrwrrlaqrh lqvyeaqmra
      121 lcqagvttda ehqlwtfwga mfycgtvftt iaalltptpw vl
//
LOCUS       XP_077485880             122 aa            linear   INV 25-NOV-2025
DEFINITION  lysosomal alpha-glucosidase-like [Amblyomma americanum].
ACCESSION   XP_077485880
VERSION     XP_077485880.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629754.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 23% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..122
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..122
                     /product="lysosomal alpha-glucosidase-like"
                     /calculated_mol_wt=14114
     CDS             1..122
                     /gene="LOC144096914"
                     /coded_by="XM_077629754.1:57..425"
                     /db_xref="GeneID:144096914"
ORIGIN      
        1 mkflvalngs deavgdlywd ggetldaqll geyvslkfra annsleicsy qykklfnstl
       61 relaimsvra lgicrrptrv eqdgyyhish rqcrwhhsnk vmdlkqilmp lrknttvrwf
      121 ms
//
LOCUS       XP_077485968             124 aa            linear   INV 25-NOV-2025
DEFINITION  annexin B11-like [Amblyomma americanum].
ACCESSION   XP_077485968
VERSION     XP_077485968.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629842.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..124
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..124
                     /product="annexin B11-like"
                     /calculated_mol_wt=13951
     Region          31..96
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     CDS             1..124
                     /gene="LOC144097019"
                     /coded_by="XM_077629842.1:1..375"
                     /db_xref="GeneID:144097019"
ORIGIN      
        1 mlmylvedak salrgdledi ivgllyplhe ylarelrkai aglgtdeecl ieilctqsnh
       61 dmrlikdhyq rifkkdlerd vigdtsgdfh rlllsmcnaq rdentpldpv rarndaavss
      121 ailp
//
LOCUS       XP_077486293             305 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097418 [Amblyomma americanum].
ACCESSION   XP_077486293
VERSION     XP_077486293.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630167.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..305
                     /product="uncharacterized protein LOC144097418"
                     /calculated_mol_wt=34196
     Region          <172..>206
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     CDS             1..305
                     /gene="LOC144097418"
                     /coded_by="XM_077630167.1:1..918"
                     /db_xref="GeneID:144097418"
ORIGIN      
        1 madrlsstvv lntvflhadl kgrpyrapdf rdalaqildl reilctgqyq mshvwmvtce
       61 sssskqklvn kaefpvkglr cmvfdpdtkn vkvkllwlpr ymehrrivea lepygtvqsv
      121 erekwrcpgm ehmetanrel sltlkdgvsa stiphtlnvy gvqalvhipg rpplclrcsr
      181 vghvrrqcrt prctqcrrfg htaencvlsy adrlrqgqws reddvasehi mdvsevvdat
      241 gelshehrid geqktstpqn nteyeatghl astppgrkpp dpgppvsvtk tavlaaqehl
      301 vatgr
//
LOCUS       XP_077486517             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097719 [Amblyomma americanum].
ACCESSION   XP_077486517
VERSION     XP_077486517.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630391.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 14% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144097719"
                     /calculated_mol_wt=14732
     CDS             1..133
                     /gene="LOC144097719"
                     /coded_by="XM_077630391.1:177..578"
                     /db_xref="GeneID:144097719"
ORIGIN      
        1 mkacqegkrp vksdlnemem asdddlgdlp scpficvkgw tfltansyti cvdthpvlha
       61 skpdeafkll ffahfafniq yqketslcle ftqraiagin pargtkvqkn ggkqhylspr
      121 vaalvtalkd ydf
//
LOCUS       XP_077486852             135 aa            linear   INV 25-NOV-2025
DEFINITION  histone-lysine N-methyltransferase set-17-like [Amblyomma
            americanum].
ACCESSION   XP_077486852
VERSION     XP_077486852.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630726.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..135
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..135
                     /product="histone-lysine N-methyltransferase set-17-like"
                     /calculated_mol_wt=14847
     Region          10..120
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(16..17,71..76,87..89,110..113)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Site            order(16..17,72..76,112)
                     /site_type="other"
                     /note="SAM binding site [polypeptide binding]"
                     /db_xref="CDD:380914"
     CDS             1..135
                     /gene="LOC144098208"
                     /coded_by="XM_077630726.1:1..408"
                     /db_xref="GeneID:144098208"
ORIGIN      
        1 mlefltsgks tvkgapvgvf tlkalpkglh fgpyhgvkgd gienggctwp vrrcgeffvv
       61 dgrpeqrnhw mndvnyspsk rtqnleaslv ngdiyyrtlr kvdpgeellv gystsftksl
      121 lrnttgrgal kedlq
//
LOCUS       XP_077487210             204 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098421 [Amblyomma americanum].
ACCESSION   XP_077487210
VERSION     XP_077487210.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631084.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..204
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..204
                     /product="uncharacterized protein LOC144098421"
                     /calculated_mol_wt=22867
     Region          89..>123
                     /region_name="fbiC"
                     /note="FO synthase; Reviewed; PRK09234"
                     /db_xref="CDD:236422"
     CDS             1..204
                     /gene="LOC144098421"
                     /coded_by="XM_077631084.1:1..615"
                     /db_xref="GeneID:144098421"
ORIGIN      
        1 mappdrsrsp ttpttppfvi mapprdpgtf cgtdgsdved winlyervsn ynrwdptlml
       61 anvifylngt arvwykthee eltswdtfke klktlfgrpd drqlaakkel atraqsstes
      121 yvayiqdila lchkvdaams etdkvghvlk giaddafhll gckdctsids iinkcrrfeq
      181 ingrrispsf srlpntaais sccr
//
LOCUS       XP_077487330             167 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098479 [Amblyomma americanum].
ACCESSION   XP_077487330
VERSION     XP_077487330.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631204.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 48% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..167
                     /product="uncharacterized protein LOC144098479"
                     /calculated_mol_wt=18575
     CDS             1..167
                     /gene="LOC144098479"
                     /coded_by="XM_077631204.1:1..504"
                     /db_xref="GeneID:144098479"
ORIGIN      
        1 mtngplvtgp pllhalmfat arleqpttae laptrvmdee eeegrvfdsh qdeadndnnq
       61 ddtndqcqec pkrmvfkevm rgiqkadplw fvaltsgpqf lvqqrspqkv nvltghrkaa
      121 aesirieksg gyvfqnqtap ssfssggtrf vkpqnfereg fhdlgwp
//
LOCUS       XP_077487451             167 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098551 [Amblyomma americanum].
ACCESSION   XP_077487451
VERSION     XP_077487451.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631325.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 23% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..167
                     /product="uncharacterized protein LOC144098551"
                     /calculated_mol_wt=17912
     CDS             1..167
                     /gene="LOC144098551"
                     /coded_by="XM_077631325.1:1..504"
                     /db_xref="GeneID:144098551"
ORIGIN      
        1 masgtarfkl caalqlvqye dllglfhnvr ggaptgppvp vgvlrrwqaa chrhrpgprg
       61 gaahaedfrg qapntpaaan rrntssspng qcdelgdgcs rhpsaasevl amamhepypg
      121 rqwrsvtfdd akrrviatdq daevdtlnmk ctvgrlptlr psrtdcc
//
LOCUS       XP_077487573             123 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098623 [Amblyomma americanum].
ACCESSION   XP_077487573
VERSION     XP_077487573.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631447.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..123
                     /product="uncharacterized protein LOC144098623"
                     /calculated_mol_wt=13629
     CDS             1..123
                     /gene="LOC144098623"
                     /coded_by="XM_077631447.1:1..372"
                     /db_xref="GeneID:144098623"
ORIGIN      
        1 msvqepqpgr qcrsvsfddg krrviatdld peveplalkt svgrpptprp rltdatplrr
       61 ptasvlpmam hepypgrqwr svtfddakrr viatdqdaev dtlnlksiig rpptlrpsrt
      121 dcc
//
LOCUS       XP_077488285             866 aa            linear   INV 25-NOV-2025
DEFINITION  piwi-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077488285
VERSION     XP_077488285.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632159.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..866
                     /product="piwi-like protein 1"
                     /calculated_mol_wt=98313
     Region          271..395
                     /region_name="PAZ_piwi_like"
                     /note="PAZ domain, Piwi_like subfamily. In multi-cellular
                     organisms, the Piwi protein appears to be essential for
                     the maintenance of germline stem cells. In the Drosophila
                     male germline, Piwi was shown to be involved in the
                     silencing of retrotransposons in the...; cd02845"
                     /db_xref="CDD:239211"
     Site            order(325,338,345,349,367,382,384)
                     /site_type="other"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]"
                     /db_xref="CDD:239211"
     Region          404..849
                     /region_name="Piwi_piwi-like_Euk"
                     /note="PIWI domain, Piwi-like subfamily found in
                     eukaryotes. This domain is found in Piwi and closely
                     related proteins, where it is believed to perform a
                     crucial role in germline cells, via RNA silencing. RNA
                     silencing refers to a group of related...; cd04658"
                     /db_xref="CDD:240016"
     Site            order(572,576,588..591,594,604,607,611,615)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240016"
     Site            order(636,638,707,841)
                     /site_type="active"
                     /db_xref="CDD:240016"
     CDS             1..866
                     /gene="LOC144099063"
                     /coded_by="XM_077632159.1:1..2601"
                     /db_xref="GeneID:144099063"
ORIGIN      
        1 mappaalprt pgvlgpatvp lpvpsvapsm paldsvegls fsidsmaigh gahlqqmaam
       61 apsavvpqqf qfaaavtpae ngdcgrpetr rrrdefavie trpvqidnkr gtsgspikvl
      121 anyfrllsmp qccihkyhve ftptvessrm rrallvdfmh mfdkclvfdg msdlkspkrl
      181 dqdinevisq rrtdgvitcv rfkwvqelap ldpellrlfn tqmrrnlerl dfvqinrhff
      241 drravasipq yglelwqglv aaigqyesgv mlvtdtlykv lrrdsvfdlm sqiqhvpnyk
      301 decvkrvagc ivmtpynnkt yrvddidwdk npactfetke gpktyadyyr dhyekrirdm
      361 rqpllvcrpk ekdlragrtq niylvpelcv ltgltdemra nvtvmrdmaq htrvepskrw
      421 rnllvfmeri knneairndm drwgirfdds lvkidarvlq pekvmqgsnt yrysaatadf
      481 sretrdrplh vavaieswiv lchrceeanv tqfvrtlmsv cppmgvkirq prlvlldddr
      541 psgfvyalrq lasggniela livlpnsrkd rydiikkeac vdlglhtqvi lartignrrn
      601 ihsvatkvav qlncklggea wcleiplvst mvigydtypd sstpnrsaga fvasinkslt
      661 rwyqrvcfhd theglakslp sllrdalrky sqcndgaspd riifyrdgvs dgqipqvmew
      721 einpivaslq glfpgvnhkl afvvvtkris trfflpsreh vtnpppgtvv dsevtrlery
      781 dfflvsqsvr qgtvapthyn vvydttglkp dhmqrlaykl thlyfnwpgt irvpapcqya
      841 hklaflagqs lhaehnprls stlfyl
//
LOCUS       XP_077488397             866 aa            linear   INV 25-NOV-2025
DEFINITION  piwi-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077488397
VERSION     XP_077488397.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632271.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..866
                     /product="piwi-like protein 1"
                     /calculated_mol_wt=98318
     Region          271..395
                     /region_name="PAZ_piwi_like"
                     /note="PAZ domain, Piwi_like subfamily. In multi-cellular
                     organisms, the Piwi protein appears to be essential for
                     the maintenance of germline stem cells. In the Drosophila
                     male germline, Piwi was shown to be involved in the
                     silencing of retrotransposons in the...; cd02845"
                     /db_xref="CDD:239211"
     Site            order(325,338,345,349,367,382,384)
                     /site_type="other"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]"
                     /db_xref="CDD:239211"
     Region          404..849
                     /region_name="Piwi_piwi-like_Euk"
                     /note="PIWI domain, Piwi-like subfamily found in
                     eukaryotes. This domain is found in Piwi and closely
                     related proteins, where it is believed to perform a
                     crucial role in germline cells, via RNA silencing. RNA
                     silencing refers to a group of related...; cd04658"
                     /db_xref="CDD:240016"
     Site            order(572,576,588..591,594,604,607,611,615)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240016"
     Site            order(636,638,707,841)
                     /site_type="active"
                     /db_xref="CDD:240016"
     CDS             1..866
                     /gene="LOC144099135"
                     /coded_by="XM_077632271.1:1..2601"
                     /db_xref="GeneID:144099135"
ORIGIN      
        1 mappaalprt pgvlgpatvp lpvpsvapsm paldsvegls fsidsmaigh gahlqqmaam
       61 apsavvpqqf qfaaavtpae ngdcgrpetr rrrdefavie trpvqidnkr gtsgspikvl
      121 anyfrllsmp qccihkyhve ftptvessrm rrallvdfmh mfdkclvfdg msdlkspkrl
      181 dqditevisq rrtdgvitcv rfkwvqelap ldpellrlfn tqmrrnlerl dfvqinrhff
      241 drravasipq yglelwqglv aaigqyesgv mlvtdtlykv lrrdsvfdlm sqiqhvpnyk
      301 decvkrvagc ivmtpynnkt yrvddidwdk npactfetke gpktyadyyr dhyekrirdm
      361 rqpllvcrpk ekdlragrtq niylvpelcv ltgltdemra nvtvmrdmaq htrvepskrw
      421 rnllvfmeri nnneairndm drwgirfdds lvkidarvlq pekvmqgsnt yrysaatadf
      481 sretrdrplh vavaveswiv lchrceeanv tqfvrtlmsv cppmgvkirq prlvlldddr
      541 psgfvyalrq lasggniela livlpnsrkd rydiikkeac vdlglhtqvi lartignrrn
      601 ihsvatkvav qlncklgcea wcleiplvst mvigydtypd sstpnrsaga fvasinkslt
      661 rwyqrvcfhd theglakslp sllrdalrky sqcndgaspd riifyrdgvs dgqipqvmew
      721 einpivaslq glfpgvnhkl afvvvtkris trfflpsreh vtnpppgtvv dsevtrlery
      781 dfflvsqsvr qgtvapthyn vvydttglkp dhmqrlaykl thlyfnwpgt irvpapcqya
      841 hklaflagqs lhaehnprls stlfyl
//
LOCUS       XP_077488503             866 aa            linear   INV 25-NOV-2025
DEFINITION  piwi-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077488503
VERSION     XP_077488503.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632377.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..866
                     /product="piwi-like protein 1"
                     /calculated_mol_wt=98285
     Region          271..395
                     /region_name="PAZ_piwi_like"
                     /note="PAZ domain, Piwi_like subfamily. In multi-cellular
                     organisms, the Piwi protein appears to be essential for
                     the maintenance of germline stem cells. In the Drosophila
                     male germline, Piwi was shown to be involved in the
                     silencing of retrotransposons in the...; cd02845"
                     /db_xref="CDD:239211"
     Site            order(325,338,345,349,367,382,384)
                     /site_type="other"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]"
                     /db_xref="CDD:239211"
     Region          404..849
                     /region_name="Piwi_piwi-like_Euk"
                     /note="PIWI domain, Piwi-like subfamily found in
                     eukaryotes. This domain is found in Piwi and closely
                     related proteins, where it is believed to perform a
                     crucial role in germline cells, via RNA silencing. RNA
                     silencing refers to a group of related...; cd04658"
                     /db_xref="CDD:240016"
     Site            order(572,576,588..591,594,604,607,611,615)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240016"
     Site            order(636,638,707,841)
                     /site_type="active"
                     /db_xref="CDD:240016"
     CDS             1..866
                     /gene="LOC144099200"
                     /coded_by="XM_077632377.1:1..2601"
                     /db_xref="GeneID:144099200"
ORIGIN      
        1 mappaalprt pgvlgpatvp lpvpsvapsm paldsvegls fsidsmaigh gahlqqmaam
       61 apsavvpqqf qfaaavtpae ngdcgrpetr rrrdefavie trpvqidnkr gtsgspikvl
      121 anyfrllsmp qccihkyhve ftptvessrm rrallvdfmh mfdkclvfdg msdlkspkrl
      181 dqditevisq rrtdgvitcv rfkwvqelap ldpellrlfn tqmrrnlerl dfvqinrhff
      241 drravasipq yglelwqglv aaigqyesgv mlvtdtlykv lrrdsvfdlm sqiqhvpnyk
      301 decvkrvagc ivmtpynnkt yrvddidwdk npactfetke gpktyadyyr dhyekrirdm
      361 rqpllvcrpk ekdlragrtq niylvpelcv ltgltdemra nvtvmrdmaq htrvepskrw
      421 rnllvfmeri knneairnnm drwgirfdds lvkidarvlq pekvmqgsnt yrysaatadf
      481 sretrdrplh vavaveswiv lchrceeanv tqfvrtlmsv cppmgvkirq prlvlldddr
      541 psgfvyalrq lasggniela livlpnsrkd rydiikkeac vdlglhtqvi lartignrrn
      601 ihsvatkvav qlncklggea wcleiplvst mvigydtypd sstpnrsaga fvasinkslt
      661 rwyqrvcfhd theglakslp sllrdalrky sqcndgaspd riifyrdgvs dgqipqvmew
      721 einpivaslq glfpgvnhkl afvvvtkris trfflpsreh vtnpppgtvv dsevtrlery
      781 dfflvsqsvr qgtvapthyn vvydttglkp dhmqrlaykl thlyfnwpgt irvpapcqya
      841 hklaflagqs lhaehnprls stlfyl
//
LOCUS       XP_077488618             866 aa            linear   INV 25-NOV-2025
DEFINITION  piwi-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077488618
VERSION     XP_077488618.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632492.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..866
                     /product="piwi-like protein 1"
                     /calculated_mol_wt=98272
     Region          271..395
                     /region_name="PAZ_piwi_like"
                     /note="PAZ domain, Piwi_like subfamily. In multi-cellular
                     organisms, the Piwi protein appears to be essential for
                     the maintenance of germline stem cells. In the Drosophila
                     male germline, Piwi was shown to be involved in the
                     silencing of retrotransposons in the...; cd02845"
                     /db_xref="CDD:239211"
     Site            order(325,338,345,349,367,382,384)
                     /site_type="other"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]"
                     /db_xref="CDD:239211"
     Region          404..849
                     /region_name="Piwi_piwi-like_Euk"
                     /note="PIWI domain, Piwi-like subfamily found in
                     eukaryotes. This domain is found in Piwi and closely
                     related proteins, where it is believed to perform a
                     crucial role in germline cells, via RNA silencing. RNA
                     silencing refers to a group of related...; cd04658"
                     /db_xref="CDD:240016"
     Site            order(572,576,588..591,594,604,607,611,615)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240016"
     Site            order(636,638,707,841)
                     /site_type="active"
                     /db_xref="CDD:240016"
     CDS             1..866
                     /gene="LOC144099271"
                     /coded_by="XM_077632492.1:1..2601"
                     /db_xref="GeneID:144099271"
ORIGIN      
        1 mappaalprt pgvlgpatvp lpvpsvapsm paldsvegls fsidsmaigh gahlqqmaam
       61 apsavvpqqf qfaaavtpae ngdcgrpetr rrrdefavie trpvqidnkr gtsgspikvl
      121 anyfrllsmp qccihkyhve ftptvessrm rrallvdfmh mfdkclvfdg msdlkspkrl
      181 dqditevisq rrtdgvitcv rfkwvqelap ldpellrlfn tqmrrnlerl dfvqinrhff
      241 drravasipq yglelwqglv aaigqyesgv mlvtdtlykv lrrdsvfdlm sqiqhvpnyk
      301 decvkrvagc ivmtpynnkt yrvddidwdk npactfetke gpktyadyyr dhyekrirdm
      361 rqpllvcrpk ekdlragrtq niylvpelcv ltgltdemra nvtvmrdmaq htrvepskrw
      421 rnllvfmeri nnneairndm drwgirfdds lvkidarvlq pekvmqgsnt yrysaatadf
      481 sretrdrplh vavaveswiv lchrceeanv tqfvrtlmsv cppmgvkirq prlvlldddr
      541 psgfvyalrq lasggniela livlpnsrkd rydiikkeac vdlglhtqvi lartignrrn
      601 ihsvatkvav qlncklggea wcleiplvst mvigydtypd sstpnrsaga fvasinkslt
      661 rwyqrvcfhd theglakslp sllrdalrky sqcndgaspd riifyrdgvs dgqipqvmew
      721 einpivaslq glfpgvnhkl afvvvtkris trfflpsreh vtnpppgtvv dsevtrlery
      781 dfflvsqsvr qgtvapthyn vvydttglkp dhmqrlaykl thlyfnwpgt irvpapcqya
      841 hklaflagqs lhaehnprls stlfyl
//
LOCUS       XP_077488732             866 aa            linear   INV 25-NOV-2025
DEFINITION  piwi-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077488732
VERSION     XP_077488732.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632606.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..866
                     /product="piwi-like protein 1"
                     /calculated_mol_wt=98302
     Region          271..395
                     /region_name="PAZ_piwi_like"
                     /note="PAZ domain, Piwi_like subfamily. In multi-cellular
                     organisms, the Piwi protein appears to be essential for
                     the maintenance of germline stem cells. In the Drosophila
                     male germline, Piwi was shown to be involved in the
                     silencing of retrotransposons in the...; cd02845"
                     /db_xref="CDD:239211"
     Site            order(325,338,345,349,367,382,384)
                     /site_type="other"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]"
                     /db_xref="CDD:239211"
     Region          404..849
                     /region_name="Piwi_piwi-like_Euk"
                     /note="PIWI domain, Piwi-like subfamily found in
                     eukaryotes. This domain is found in Piwi and closely
                     related proteins, where it is believed to perform a
                     crucial role in germline cells, via RNA silencing. RNA
                     silencing refers to a group of related...; cd04658"
                     /db_xref="CDD:240016"
     Site            order(572,576,588..591,594,604,607,611,615)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240016"
     Site            order(636,638,707,841)
                     /site_type="active"
                     /db_xref="CDD:240016"
     CDS             1..866
                     /gene="LOC144099343"
                     /coded_by="XM_077632606.1:1..2601"
                     /db_xref="GeneID:144099343"
ORIGIN      
        1 mappaalprt pgvlgpatvp lpvpsvapsm paldsvegls fsidsmaigh gahlqqmaam
       61 apsavvpqqf qfaaavtpae ngdcgrpetr rrrdefavie trpvqidnkr gtsgspikvl
      121 anyfrllsmp qccihkyhve ftptvessrm rrallvdfmh mfdkclvfdg msdlkspkrl
      181 dqditevisq rrtdgvitcv rfkwvqelap ldpellrlfn tqmrrnlerl dfvqinrhff
      241 drravasipq yglelwqglv aaigqyesgv mlvtdtlykv lrrdsvfdlm sqiqhvpnyk
      301 decvkrvagc ivmtpynnkt yrvddidwdk npactfetke gpktyadyyr dhyekrirdm
      361 rqpllvcrpk ekdlragrtq niylvpelcv ltgltdemra nvtvmrdmaq htrvepskrw
      421 rnllvfmeri nnneairndm drwgirfdds lvkidarvlq pekvmqgsnt yrysaatadf
      481 sretrdrplh vavaveswiv lchrceeanv tqfvrtlmsv cppmgvkirq prlvlldddr
      541 psgfvyalrq ltsggniela livlpnsrkd rydiikkeac vdlglhtqvi lartignrrn
      601 ihsvatkvav qlncklggea wcleiplvst mvigydtypd sstpnrsaga fvasinkslt
      661 rwyqrvcfhd theglakslp sllrdalrky sqcndgaspd riifyrdgvs dgqipqvmew
      721 einpivaslq glfpgvnhkl afvvvtkris trfflpsreh vtnpppgtvv dsevtrlery
      781 dfflvsqsvr qgtvapthyn vvydttglkp dhmqrlaykl thlyfnwpgt irvpapcqya
      841 hklaflagqs lhaehnprls stlfyl
//
LOCUS       XP_077488857             866 aa            linear   INV 25-NOV-2025
DEFINITION  piwi-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077488857
VERSION     XP_077488857.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632731.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..866
                     /product="piwi-like protein 1"
                     /calculated_mol_wt=98214
     Region          271..395
                     /region_name="PAZ_piwi_like"
                     /note="PAZ domain, Piwi_like subfamily. In multi-cellular
                     organisms, the Piwi protein appears to be essential for
                     the maintenance of germline stem cells. In the Drosophila
                     male germline, Piwi was shown to be involved in the
                     silencing of retrotransposons in the...; cd02845"
                     /db_xref="CDD:239211"
     Site            order(325,338,345,349,367,382,384)
                     /site_type="other"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]"
                     /db_xref="CDD:239211"
     Region          404..849
                     /region_name="Piwi_piwi-like_Euk"
                     /note="PIWI domain, Piwi-like subfamily found in
                     eukaryotes. This domain is found in Piwi and closely
                     related proteins, where it is believed to perform a
                     crucial role in germline cells, via RNA silencing. RNA
                     silencing refers to a group of related...; cd04658"
                     /db_xref="CDD:240016"
     Site            order(572,576,588..591,594,604,607,611,615)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240016"
     Site            order(636,638,707,841)
                     /site_type="active"
                     /db_xref="CDD:240016"
     CDS             1..866
                     /gene="LOC144099410"
                     /coded_by="XM_077632731.1:1..2601"
                     /db_xref="GeneID:144099410"
ORIGIN      
        1 mappaalprt pgvlgpatvp lpvpsvapsm paldsvegls fsidsmaigh gahlqqmaam
       61 apsavvpqqf qfaaavtpae ngdcgrpetr rrrdefavie trpvqidnkr gtsgspikvl
      121 anyfrllsmp qccihkyhve ftppvessrm rrallvdfmh mfdkclvfdg msdlkspkrl
      181 dqditevisq rrtdgvitcv rfkwvqelap ldpellrlfn tqmrrnlerl dfvqinrhff
      241 drravasipq yglelwqglv aaigqyesgv mlvtdtlykv lrrdsvfdlm sqiqhvpnyk
      301 decvkrvagc ivmtpynnkt yrvddidwdk npactfetke gpktyadyyr dhyekrirdm
      361 rqpllvcrpk ekdlragrte niylvpelcv ltgltdemra nvtvmrdmaq htrvepskrw
      421 rnllvfmeri nnneairndm drwgirfdds lvkidarvlq pekvmqgsnt yrysaatadf
      481 sretrdrplh vavaveswiv lchrceeanv tqfvrtlmsv cppmgvkirq prlvlleddr
      541 psgfvyalrq lasggniela livlpnsrkd rydiikkeac vdlglhtqvi lartignrrn
      601 ihsvatkvav qlncklggea wcleiplvst mvigydtypd sstpnrsaga fvasinkslt
      661 rwyqrvcfhd theglakslp sllrdalrky sqcndgaspd riifyrdgvs dgqipqvmew
      721 einpivaslq glfpgvnhkl afvvvtkris trfflpsreh vtnpppgtvv dsevtrlery
      781 dfflvsqsvr qgtvapthyn vvydttglkp dhmqrlaykl thlyfnwpgt isvpapcqya
      841 hklaflagqs lhaehnprls stlfyl
//
LOCUS       XP_077489092             206 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099541 [Amblyomma americanum].
ACCESSION   XP_077489092
VERSION     XP_077489092.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632966.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 23% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..206
                     /product="uncharacterized protein LOC144099541"
                     /calculated_mol_wt=22730
     Region          11..100
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..206
                     /gene="LOC144099541"
                     /coded_by="XM_077632966.1:162..782"
                     /db_xref="GeneID:144099541"
ORIGIN      
        1 myknyssgrh caivgcknnq rkrklvmden caehlckrrv cmcgvyslhr fpaapekcre
       61 weialnrknf kpsklarvcs vhfidgkpta lnpcpmlklg yakkvtrgrr pilrtlqpka
      121 asvqimdsqa tqhpvsavtp aqaasaasss twdtsdllqq tsdsdecyss vldlscrgpv
      181 stqegrasqk cnkscqaemq vcscqq
//
LOCUS       XP_077489138             207 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099629 [Amblyomma americanum].
ACCESSION   XP_077489138
VERSION     XP_077489138.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633012.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..207
                     /product="uncharacterized protein LOC144099629"
                     /calculated_mol_wt=23107
     Region          <84..152
                     /region_name="COG3335"
                     /note="Transposase [Mobilome: prophages, transposons]"
                     /db_xref="CDD:442564"
     CDS             1..207
                     /gene="LOC144099629"
                     /coded_by="XM_077633012.1:1..624"
                     /db_xref="GeneID:144099629"
ORIGIN      
        1 mdgnyneksf tdkllpnfpa nsivimnnar hlsvalekgp tksmrksdiq lwltkkgvpw
       61 sqdmvrtell gpsqkvntps vvyridtlaa thghevlrlp pyhcqfnpie lvwgqvkghi
      121 asrnkcftlv evenllpeal asvsqknwrn ccvhvgkvea eawqrdtied aeikavvfst
      181 gdpcnsssdw ssthfsdedf sgieelv
//
LOCUS       XP_077489414              66 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100271 [Amblyomma americanum].
ACCESSION   XP_077489414
VERSION     XP_077489414.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633288.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..66
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..66
                     /product="uncharacterized protein LOC144100271"
                     /calculated_mol_wt=7118
     CDS             1..66
                     /gene="LOC144100271"
                     /coded_by="XM_077633288.1:1..201"
                     /db_xref="GeneID:144100271"
ORIGIN      
        1 mklkpdsvgv mvparrvpva lqdkvaaelq rmeaqgvisk pevrylghil steglrldpg
       61 rvtdil
//
LOCUS       XP_077489564              66 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100474 [Amblyomma americanum].
ACCESSION   XP_077489564
VERSION     XP_077489564.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633438.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..66
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..66
                     /product="uncharacterized protein LOC144100474"
                     /calculated_mol_wt=7118
     CDS             1..66
                     /gene="LOC144100474"
                     /coded_by="XM_077633438.1:1..201"
                     /db_xref="GeneID:144100474"
ORIGIN      
        1 mklkpdsvgv mvparrvpva lqdkvaaelq rmeaqgvisk pevrylghil steglrldpg
       61 rvtdil
//
LOCUS       XP_077489679             421 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100675 [Amblyomma americanum].
ACCESSION   XP_077489679
VERSION     XP_077489679.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633553.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..421
                     /product="uncharacterized protein LOC144100675"
                     /calculated_mol_wt=46894
     Region          245..296
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     CDS             1..421
                     /gene="LOC144100675"
                     /coded_by="XM_077633553.1:1..1266"
                     /db_xref="GeneID:144100675"
ORIGIN      
        1 mdfkkvdree lllmdsaatl dvvrpsyvts edftgavtwi kqvleehsvc lpmarveisg
       61 pfgklvteaa vsktvpleyp ylfsnrsdrl lrergqifge gkvqaltrsk trqlaaqltq
      121 dmgqgeaeig aepeimepnl vagqgvevva rsgdidaaaa psepqeiepv gasilapssr
      181 sierlleidr dilkaeqknd pslrrlhata ekgiarrnit ilekggllyr hyrdrkgkpc
      241 dqlvipekyr adllrlchgn swsghlgmkk tkerllmeyy wpgcfkdver yvkscdaseq
      301 dteptttslh eeecpvskvm rgiqntdpmw lgaltwgpqs veqkrspqev nvltdrrkaa
      361 aetrrieesg gyvfqnqtva ppfssggtpf cephiyqqka stscltlesr fdkrltasgp
      421 d
//
LOCUS       XP_077489773             348 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100768 [Amblyomma americanum].
ACCESSION   XP_077489773
VERSION     XP_077489773.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633647.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 20% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..348
                     /product="uncharacterized protein LOC144100768"
                     /calculated_mol_wt=39038
     Region          248..304
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     CDS             1..348
                     /gene="LOC144100768"
                     /coded_by="XM_077633647.1:1..1047"
                     /db_xref="GeneID:144100768"
ORIGIN      
        1 msqgyahvng kpckvlrdsa atldvvrpsy vtsedftgav twikqvleeh svclpmarve
       61 isgpfgklvt eaavsktvpl eypylfsnrs drllrergqf fgegkvqalt rsktrqlaaq
      121 ltqdmgqgea eigaepeikk pnlvaeqgve vvarsgdida aaapsepqei epvgasilap
      181 ssrsierlle idrdmlkaeq kndpslrrlh ataekgiarr nitilekggl lyrhyrdrkg
      241 kpcdqlvipe kyradllrlc hgnswsghlg mkktkerllm eyywpgcfkd veryvkscda
      301 cqrvgkpenk mevqwegpve iqhrlsainy vlkkigkrne vtiyhlql
//
LOCUS       XP_077490341             309 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101141 [Amblyomma americanum].
ACCESSION   XP_077490341
VERSION     XP_077490341.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634215.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 18% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..309
                     /product="uncharacterized protein LOC144101141"
                     /calculated_mol_wt=33666
     CDS             1..309
                     /gene="LOC144101141"
                     /coded_by="XM_077634215.1:117..1046"
                     /db_xref="GeneID:144101141"
ORIGIN      
        1 mddndnsqpf pmdiaymred llvgcllqpf hdddvecgrr nrwsssccks rhdadlsgdg
       61 ntgtqlrggn sqtvrspkkf ngavknfgra dafpfpggdv qsndqnlldi gylengdkss
      121 gaqsfhknsd viilrkfpgt rvnfgladsg flgedgvqgd teelmdtdeg pevaersqgf
      181 llceedskhf ippgdqespr hvrgfpfmae gfpnkgille tgfkciqdkg gdvqdsvevm
      241 hfecegrpda tgdtgkdyrs stpsrisptt tshdkspppt hltiqkafnt ptlqtlpptr
      301 spcncgtkk
//
LOCUS       XP_077490452             109 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101219 [Amblyomma americanum].
ACCESSION   XP_077490452
VERSION     XP_077490452.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634326.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 15% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..109
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..109
                     /product="uncharacterized protein LOC144101219"
                     /calculated_mol_wt=11868
     CDS             1..109
                     /gene="LOC144101219"
                     /coded_by="XM_077634326.1:6..335"
                     /db_xref="GeneID:144101219"
ORIGIN      
        1 mtgrgrpavd dvgmdwpnsf glavyasysm diaringrtl gvhiltrgcf ggqdyvaklk
       61 avniegenvr pqaapkvlrr alrkrrspqv hrasgtaace rrarescrt
//
LOCUS       XP_077490941             165 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101647 [Amblyomma americanum].
ACCESSION   XP_077490941
VERSION     XP_077490941.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634815.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..165
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..165
                     /product="uncharacterized protein LOC144101647"
                     /calculated_mol_wt=18440
     CDS             1..165
                     /gene="LOC144101647"
                     /coded_by="XM_077634815.1:1..498"
                     /db_xref="GeneID:144101647"
ORIGIN      
        1 maaccddsfs efvdfvrrae dvtsdeacsf vhplrrnlrd rqnpiqvynn qeflaryrfs
       61 kaavlellsm lplhwntdgr gcpvppllql lvtlpfygag afqvvsgglv nvsqptvsrv
      121 vtrvstmiaa tlypalvkfp eagkmhqvmy dlnkiaqfpr sdwlc
//
LOCUS       XP_077491045             109 aa            linear   INV 25-NOV-2025
DEFINITION  endoplasmic reticulum-Golgi intermediate compartment protein 1-like
            [Amblyomma americanum].
ACCESSION   XP_077491045
VERSION     XP_077491045.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634919.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..109
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..109
                     /product="endoplasmic reticulum-Golgi intermediate
                     compartment protein 1-like"
                     /calculated_mol_wt=12097
     Region          5..92
                     /region_name="ERGIC_N"
                     /note="Endoplasmic Reticulum-Golgi Intermediate
                     Compartment (ERGIC); pfam13850"
                     /db_xref="CDD:464000"
     CDS             1..109
                     /gene="LOC144101743"
                     /coded_by="XM_077634919.1:1..330"
                     /db_xref="GeneID:144101743"
ORIGIN      
        1 mafdvrrfdi ylkipkdltq ptvtgavist lscffiyilf lsefisymsp elvselyvdn
       61 psstekipms iditllkldc svvgldiqdd mgrdeagfve dtcglrlsv
//
LOCUS       XP_077491440             182 aa            linear   INV 25-NOV-2025
DEFINITION  Krueppel-like factor 1 [Amblyomma americanum].
ACCESSION   XP_077491440
VERSION     XP_077491440.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635314.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 42% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..182
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..182
                     /product="Krueppel-like factor 1"
                     /calculated_mol_wt=20834
     Region          <8..163
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          83..103
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(88,90,92,94..95,98..99,102,118,120,124..125,
                     128..129,132,146,148,150,152..153,156..157,160)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          111..133
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          141..161
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..182
                     /gene="LOC144102025"
                     /coded_by="XM_077635314.1:1..549"
                     /db_xref="GeneID:144102025"
ORIGIN      
        1 mtgkeraspi frpwaqqqse arivqpepvy llpppavsal qpafpsqlta wevaqhhhap
       61 vllqqelaar wkakkqrpkr fqcphckvsf snngqlkghv rihtgerpfa cehegcgktf
      121 trneeltrhr rihtglrpfp cplcqkpfgr kdhlkkhvkt herallpglp yffgatpdff
      181 fm
//
LOCUS       XP_077491538             376 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102094 [Amblyomma americanum].
ACCESSION   XP_077491538
VERSION     XP_077491538.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635412.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 30% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..376
                     /product="uncharacterized protein LOC144102094"
                     /calculated_mol_wt=40978
     Region          <254..>292
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     CDS             1..376
                     /gene="LOC144102094"
                     /coded_by="XM_077635412.1:1..1131"
                     /db_xref="GeneID:144102094"
ORIGIN      
        1 mtvpfmkcny vaenvqpivr aiesldseev lvchvnsimp pvrkirlpat erdgraptgs
       61 vgaataamsg rgnrilasds dyqvvlpicp tgrfventvf lhgdirarpf rvedfrdala
      121 plslmpdvia lgayrmnhvw avtfkdadav kkivsagdlq vkntrcfvid tanrdvrmkl
      181 hwllhsvpdd dvrlafavsg kvtdisrerw ralgvsdkss ttrlvtlkln agvqlddlph
      241 qvsvsgelal vvvpgraplc lrcrgtghir recriprcgt crrfgneegq cprtyasvtg
      301 pgngdrssel ymdeadaeea tqaekpaavl tkptldvqva aepcpsvsty pslkkdakqe
      361 esaglqdvsk eltted
//
LOCUS       XP_077491640             410 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102168 [Amblyomma americanum].
ACCESSION   XP_077491640
VERSION     XP_077491640.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635514.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..410
                     /product="uncharacterized protein LOC144102168"
                     /calculated_mol_wt=44259
     Region          <212..>252
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     CDS             1..410
                     /gene="LOC144102168"
                     /coded_by="XM_077635514.1:1..1233"
                     /db_xref="GeneID:144102168"
ORIGIN      
        1 mirlpaterd grdimssvga vsaaqsgrgn rffaatadhy qvilpnlpsg rivantvfmh
       61 gdprarpyrv edyrdtlanl galpdvvalg ayqmnhvwav tmttadaaqk lllavdvkvk
      121 drpcllidpn nrevrvklhw llhgvtdedv rvalspygkv levtrekwra lgvtekgstt
      181 rtvglklhad vkiddiphql kiageltlvv vpsraplclr ckstghirrd ckvprcsrcr
      241 rfghvdadca ktyasvagpv qandisdhlm deedseetar tsrnlvtqga ssnfqgeqli
      301 dggdkpadkr rhddaeggne kdeasttghp spgggekppt epapvtvmds dsisaaakrp
      361 hdagengkel svvvadeppa kttvgrrpsf rplpntsaar ktaetppmpp
//
LOCUS       XP_077491737             185 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102272 [Amblyomma americanum].
ACCESSION   XP_077491737
VERSION     XP_077491737.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635611.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 16% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..185
                     /product="uncharacterized protein LOC144102272"
                     /calculated_mol_wt=21831
     Region          <49..105
                     /region_name="zf-RVT"
                     /note="zinc-binding in reverse transcriptase; pfam13966"
                     /db_xref="CDD:433612"
     CDS             1..185
                     /gene="LOC144102272"
                     /coded_by="XM_077635611.1:1..558"
                     /db_xref="GeneID:144102272"
ORIGIN      
        1 mlhvrfsmey lcnvkkkkly vdlvgtmlpl piyrlphiag pgadvlkrfk kmpilpaiks
       61 fffklhtntl svktwmhdkg ifvpwstncf lchnpetieh vfldcwdpif fwdvlqrtlk
      121 kdlpldaygi rflpfhktdy fpfdavmllg lnslwktrme fnnadeqvkp vrehfiqnvv
      181 rfrea
//
LOCUS       XP_077491818             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077491818
VERSION     XP_077491818.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635692.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=49636
     Region          1..430
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144102377"
                     /coded_by="XM_077635692.1:301..1632"
                     /db_xref="GeneID:144102377"
ORIGIN      
        1 mreivyiqtg qcgnqigakf wevisdehgi dpsgeyygds dlqlerinvy yneapggkyv
       61 prsilvdlep gtmdavrsgp fgqlfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeakscdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv psskvsdtvv
      181 epynatlslh qlventdetf cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrgcqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmreiddqm lniqnkdssy fvewipnnvk tavcdipprg
      361 lkmsatfian staiqelfkr iseqftamfr rkaflhwytg egmdelefte aesnlnelvs
      421 eyqqyqeatv edegefeete aea
//
LOCUS       XP_077491893             410 aa            linear   INV 25-NOV-2025
DEFINITION  palmitoyltransferase ZDHHC20-B-like [Amblyomma americanum].
ACCESSION   XP_077491893
VERSION     XP_077491893.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635767.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..410
                     /product="palmitoyltransferase ZDHHC20-B-like"
                     /calculated_mol_wt=45461
     Region          130..249
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; pfam01529"
                     /db_xref="CDD:396215"
     CDS             1..410
                     /gene="LOC144102481"
                     /coded_by="XM_077635767.1:37..1269"
                     /db_xref="GeneID:144102481"
ORIGIN      
        1 matssggses arrrrsnhef vawlpvamvv glfawayyvy ifvfcgslvk egaqrfafst
       61 vfhlllllcl wsfvqttvta vppipgyfgl sesdqrlleq caddeargef ldilaenrgv
      121 ltrgtsggvr fcercqqvkp drahhcsqcl rcvpkmdhhc pwfnkcvcfs tykfflltif
      181 yvvalcvfgl asathhvada wssnastyvt lnctflyafg vmlvlvlgsf lylhfimvcn
      241 nvttledlrp hlfkddrdsf digfrcniae vfghrkalwf lpvftslgdg arfptrlhpd
      301 ryvygppvsv mveerrpsvp vpplpvadev pelpvltscr rpvpstegpl ggltltpfva
      361 pprgslsflp pttitivppt eaaasvrlse skkdlppivv asaftsmevr
//
LOCUS       XP_077492105             161 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103027 [Amblyomma americanum].
ACCESSION   XP_077492105
VERSION     XP_077492105.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635979.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 25% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..161
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..161
                     /product="uncharacterized protein LOC144103027"
                     /calculated_mol_wt=16607
     CDS             1..161
                     /gene="LOC144103027"
                     /coded_by="XM_077635979.1:100..585"
                     /db_xref="GeneID:144103027"
ORIGIN      
        1 mspatelvga vadhmatrlp npfdsdgahv geavlslppa alleamvngn etsqddvpaq
       61 spllnsrpin edelpvqeec lnasrrtvvv pnqsssegip ptqtartrag gprvaavert
      121 lapelaarit aieaeelrsg arksctismc nsagaswpss g
//
LOCUS       XP_077492128             539 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103186 [Amblyomma americanum].
ACCESSION   XP_077492128
VERSION     XP_077492128.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636002.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..539
                     /product="uncharacterized protein LOC144103186"
                     /calculated_mol_wt=57992
     Region          <2..65
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Region          266..379
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     Site            order(269..272,301..303,306,330,369)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260008"
     CDS             1..539
                     /gene="LOC144103186"
                     /coded_by="XM_077636002.1:1..1620"
                     /db_xref="GeneID:144103186"
ORIGIN      
        1 malqraldav ksfltskglv lsatkseail vhprgarata eirtlqidgt nlpwrqqvry
       61 lgltidhrlt wipadkplll hldrvgrtav rllargngcs atwairlfea agtsavlyal
      121 plvtlkaarl nrleiarrkw irrllgvprt sqtpatlaea gvlplnllll qrglthtdrl
      181 hhvpdgeapc arllgrphsr tgmlagayle slgrpnshla lpplptrppl qvsldfggts
      241 krrtpaaaik qaaaalleec ggstllftdg svlpasgraa aalvapqlgv tktcrlsfpg
      301 sstaaelagl hlaadlmaal ppgpvavlcd srsalqlltr phdavattsq lrarlhaled
      361 aghritlhwv pghsgiegny ladaaaksah adgspvslav tqhdfarsll lqvvrtpypd
      421 rriaarkpfr rlsdqlprrd rslflrlrig ctwtpsrlyv hgrasspacp scgdtgslgh
      481 lllaypthdl prqrlesgyr alglpttted dflfpvrnql qahrvllsfl gesglslll
//
LOCUS       XP_077492256             373 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103374 [Amblyomma americanum].
ACCESSION   XP_077492256
VERSION     XP_077492256.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636130.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..373
                     /product="uncharacterized protein LOC144103374"
                     /calculated_mol_wt=41702
     Region          5..95
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..373
                     /gene="LOC144103374"
                     /coded_by="XM_077636130.1:1..1122"
                     /db_xref="GeneID:144103374"
ORIGIN      
        1 mpnkccvpgc tgnydtgrki hvfflpkdyd tfkkwlraip rkdfvptsht kacadhfdas
       61 cietttsytd prrgnvltvr lpvprlrrgs vptvfpncps hlsapdnskr eapdanrsrl
      121 easqlalavk gslasyeaeq erhqfsslee qkarlqvasv svqwtvihke kctmflnitn
      181 drepwlkasl tvfenlqvpa cyqgsaiknl gsavvpdsvc kvsslleilk nlcmlsdesg
      241 ercssrhlsl avnslldkle gsinenkkga vnflkeqltl lsaeriqyst qlmmfacily
      301 tisphaykyl rktstlalph pstirevcss vqmrpeidsc datflqyvsq rfthlqphek
      361 tapsktgnsv rfk
//
LOCUS       XP_077492494             346 aa            linear   INV 25-NOV-2025
DEFINITION  PR domain zinc finger protein 14-like [Amblyomma americanum].
ACCESSION   XP_077492494
VERSION     XP_077492494.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636368.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 40% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..346
                     /product="PR domain zinc finger protein 14-like"
                     /calculated_mol_wt=38703
     Region          10..120
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(16..17,71..76,87..89,110..113)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Site            order(16..17,72..76,112)
                     /site_type="other"
                     /note="SAM binding site [polypeptide binding]"
                     /db_xref="CDD:380914"
     Region          147..163
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          178..198
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(183,185,187,189..190,193..194,197,211,213,217..218,
                     221..222,225,239,241,243,245..246,249..250)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          205..226
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          206..226
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          218..241
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          234..250
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          263..279
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..346
                     /gene="LOC144103675"
                     /coded_by="XM_077636368.1:1..1041"
                     /db_xref="GeneID:144103675"
ORIGIN      
        1 mlefltsgks tvkgapvgvf tlkalpkglh fgpyhgvkgd gienggctwp vrrcgeffvv
       61 dgrpeqrnhw mndvnyspsk rtqnleaslv ngdiyyrtlr kvdpgeellv gystsftksl
      121 lgnttgrgal kegdvsivsl dslflacgmc gdlfttqykm emhtrqkhwr ntkgihqcth
      181 ctftstrkad iirherthvn krnsvcslcq krfsqpgnlk vhmmihtgek pyecgqcgkr
      241 freraharrh eevihsrryp lhcprcgada advtrlrrht ckqlgttegk egfkrgrgrp
      301 rlmadgtapt vrprkttega pqqggmkrgr grprkalaqd asmpsp
//
LOCUS       XP_077492708             215 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103847 [Amblyomma americanum].
ACCESSION   XP_077492708
VERSION     XP_077492708.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636582.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 50% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..215
                     /product="uncharacterized protein LOC144103847"
                     /calculated_mol_wt=24286
     CDS             1..215
                     /gene="LOC144103847"
                     /coded_by="XM_077636582.1:1..648"
                     /db_xref="GeneID:144103847"
ORIGIN      
        1 mnfscpelqr gtgvveglpf hplhlwsrmr tlgtehrilq wnqprkkygd srepkarcer
       61 cgacrrdrqf lyhkglyvtg eelppivgpk arqvqifqas qsqsapnsss sdvqgtsrtg
      121 istaqgvapg egitneeqea pcprrsreee gmatekyhrw kpayfsnetw rklpddlkkr
      181 ygnvnkdgsm kerkgvptge eepmvvepse pylka
//
LOCUS       XP_077492802             138 aa            linear   INV 25-NOV-2025
DEFINITION  histone-lysine N-methyltransferase set-17-like [Amblyomma
            americanum].
ACCESSION   XP_077492802
VERSION     XP_077492802.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636676.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..138
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..138
                     /product="histone-lysine N-methyltransferase set-17-like"
                     /calculated_mol_wt=15067
     Region          10..120
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(16..17,71..76,87..89,110..113)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Site            order(16..17,72..76,112)
                     /site_type="other"
                     /note="SAM binding site [polypeptide binding]"
                     /db_xref="CDD:380914"
     CDS             1..138
                     /gene="LOC144103924"
                     /coded_by="XM_077636676.1:1..417"
                     /db_xref="GeneID:144103924"
ORIGIN      
        1 mlefltsgks tikgapvgvf tlkalpkglh fgpyhgvkad gienggctwp vrrcgeffvv
       61 dgrpeqrnhw mndvnyspsk rsqnleaslv ngdiyyrtlr kvdpgeellv gystsftkrl
      121 lgnttgrgal neahllgd
//
LOCUS       XP_077493017             312 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104065 [Amblyomma americanum].
ACCESSION   XP_077493017
VERSION     XP_077493017.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636891.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 36% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..312
                     /product="uncharacterized protein LOC144104065"
                     /calculated_mol_wt=34870
     Region          44..153
                     /region_name="RNase_HI_RT_Ty3"
                     /note="Ty3/Gypsy family of RNase HI in long-term repeat
                     retroelements; cd09274"
                     /db_xref="CDD:260006"
     Region          <188..>287
                     /region_name="PRK14959"
                     /note="DNA polymerase III subunits gamma and tau;
                     Provisional"
                     /db_xref="CDD:184923"
     CDS             1..312
                     /gene="LOC144104065"
                     /coded_by="XM_077636891.1:1..939"
                     /db_xref="GeneID:144104065"
ORIGIN      
        1 meqrveqvat rvdianavkv gteqdptqnr etkavmarhi pdfkddkdkw qppigfrsrt
       61 itqaerkysq lerealalvf gvtkfrdyhl greftlltdy qpllsllrsd kqtppvaaar
      121 iqrwahhlga ypyrlqyapg rqmlnadals rlpqrfpead ppalpgpken kydmlpllls
      181 evyarnygag siwtpshvet tsgarmvtve tsdgvvqrhv cqvrprpeap rdnpavttat
      241 thcsapdqat qlqhpetvsp degisarrtp ngtgspdlpa pdipvaplnt gvaqqtlrrs
      301 trehkpvqrf hf
//
LOCUS       XP_077493237             215 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104207 [Amblyomma americanum].
ACCESSION   XP_077493237
VERSION     XP_077493237.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637111.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 50% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..215
                     /product="uncharacterized protein LOC144104207"
                     /calculated_mol_wt=24286
     CDS             1..215
                     /gene="LOC144104207"
                     /coded_by="XM_077637111.1:1..648"
                     /db_xref="GeneID:144104207"
ORIGIN      
        1 mnfscpelqr gtgvveglpf hplhlwsrmr tlgtehrilq wnqprkkygd srepkarcer
       61 cgacrrdrqf lyhkglyvtg eelppivgpk arqvqifqas qsqsapnsss sdvqgtsrtg
      121 istaqgvapg egitneeqea pcprrsreee gmatekyhrw kpayfsnetw rklpddlkkr
      181 ygnvnkdgsm kerkgvptge eepmvvepse pylka
//
LOCUS       XP_077493346             138 aa            linear   INV 25-NOV-2025
DEFINITION  histone-lysine N-methyltransferase set-17-like [Amblyomma
            americanum].
ACCESSION   XP_077493346
VERSION     XP_077493346.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637220.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..138
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..138
                     /product="histone-lysine N-methyltransferase set-17-like"
                     /calculated_mol_wt=15067
     Region          10..120
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(16..17,71..76,87..89,110..113)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Site            order(16..17,72..76,112)
                     /site_type="other"
                     /note="SAM binding site [polypeptide binding]"
                     /db_xref="CDD:380914"
     CDS             1..138
                     /gene="LOC144104284"
                     /coded_by="XM_077637220.1:1..417"
                     /db_xref="GeneID:144104284"
ORIGIN      
        1 mlefltsgks tikgapvgvf tlkalpkglh fgpyhgvkad gienggctwp vrrcgeffvv
       61 dgrpeqrnhw mndvnyspsk rsqnleaslv ngdiyyrtlr kvdpgeellv gystsftkrl
      121 lgnttgrgal neahllgd
//
LOCUS       XP_077493912             150 aa            linear   INV 25-NOV-2025
DEFINITION  cell growth-regulating nucleolar protein [Amblyomma americanum].
ACCESSION   XP_077493912
VERSION     XP_077493912.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637786.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 23% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..150
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..150
                     /product="cell growth-regulating nucleolar protein"
                     /calculated_mol_wt=17080
     Region          32..59
                     /region_name="zf-LYAR"
                     /note="LYAR-type C2HC zinc finger; pfam08790"
                     /db_xref="CDD:462605"
     CDS             1..150
                     /gene="LOC144104625"
                     /coded_by="XM_077637786.1:576..1028"
                     /db_xref="GeneID:144104625"
ORIGIN      
        1 mvvftcnacg dslkknqvek hyytkcrscd mltcidcnkd fwgdaykehi kcvteaeryg
       61 gknfkppvfk gevkqqewte llskiiathd vaprvrgvfe rmqsfnnvpr kekpfkieir
      121 parfdpstva saanraamny csgwkqdsav
//
LOCUS       XP_077494019             403 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104701 [Amblyomma americanum].
ACCESSION   XP_077494019
VERSION     XP_077494019.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637893.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 25% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..403
                     /product="uncharacterized protein LOC144104701"
                     /calculated_mol_wt=43266
     CDS             1..403
                     /gene="LOC144104701"
                     /coded_by="XM_077637893.1:1..1212"
                     /db_xref="GeneID:144104701"
ORIGIN      
        1 mtssvgaast ampdcgnrsf asalssgsey rvvlpslptg rfvvnivflh advrarpyrv
       61 edfrdafaql sllpevvalg ayrmnhvwav tfkddetlkq ivragelkvk sarrlvtdpa
      121 nqevrmkvhw llhsvpdedv rlafapfgqv tdvsrerwrv hgvsdkgstt rlvtlrlnag
      181 vkledlphqv svagelalvv vpgraslclr crgtghirre criprcgswr rfgheeeqca
      241 rtyanvtgrv ssedsselfm deadaeeask aaskpatqes tltppqtdqp gtlpkvtaat
      301 gqlvvapvdt tpvasmprsa setpmdsses vtqssaepmd vlsaatksaa gkrthneagc
      361 sapqldaggg depptkapgm krstlkprpn vpvdsrqagk ppp
//
LOCUS       XP_077494249             464 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104866 [Amblyomma americanum].
ACCESSION   XP_077494249
VERSION     XP_077494249.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638123.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 42% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..464
                     /product="uncharacterized protein LOC144104866"
                     /calculated_mol_wt=49626
     Region          <247..>281
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     Region          <303..461
                     /region_name="LGT"
                     /note="Prolipoprotein diacylglyceryl transferase; cl00478"
                     /db_xref="CDD:469786"
     CDS             1..464
                     /gene="LOC144104866"
                     /coded_by="XM_077638123.1:1..1395"
                     /db_xref="GeneID:144104866"
ORIGIN      
        1 mrehswasff gaentigtpc rfatffmclc gatfyfrpse rdgrrimisg gaataalagr
       61 gnriegnddt dyqvilpqlp tggvvlntlf lhgdvrvrpf rvedfrdala qagtlpdvvv
      121 lgayqinhvw avtfktaega krllafkelk vkgrrcliid pedqqfklrl hwmlegvade
      181 dirtafaafg kvtevtrerw rvqgvhdkgs ttrtvllklk pglkvddlph qlrvagelal
      241 vvapgrpmqc lrchgtghvr reckvprcsk crryghadsd cirtyavatg lamgvdtteh
      301 imgvaeteea ekgtdvttkp avttessghp sgdgnttsgg gkepgteiaa gtnegsssdg
      361 tsnpaknvdq pptseeqmdq sgarvttvaa pakrphdltd negdkpaagg veeppaktaq
      421 srrstlrptp nlaagkriae kalplppqqn qasapdgsgg gngv
//
LOCUS       XP_077494401             167 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105054 [Amblyomma americanum].
ACCESSION   XP_077494401
VERSION     XP_077494401.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638275.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 22% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..167
                     /product="uncharacterized protein LOC144105054"
                     /calculated_mol_wt=18194
     CDS             1..167
                     /gene="LOC144105054"
                     /coded_by="XM_077638275.1:119..622"
                     /db_xref="GeneID:144105054"
ORIGIN      
        1 mddvmrlagv vrervkchaf edghaqqadg qssiwtsedn gcpsprlpph cypvavpcgs
       61 nllpgkgrrr lwrqlpdvsa vqtaplrrwk rrytapsatl lpcsstlrfq paawkrptal
      121 mvqlpdapav qtapsaplet vvysvlhhaa tlyqytacss nlppgnd
//
LOCUS       XP_077494460             287 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105148 [Amblyomma americanum].
ACCESSION   XP_077494460
VERSION     XP_077494460.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638334.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..287
                     /product="uncharacterized protein LOC144105148"
                     /calculated_mol_wt=32556
     Region          58..>154
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     CDS             1..287
                     /gene="LOC144105148"
                     /coded_by="XM_077638334.1:1..864"
                     /db_xref="GeneID:144105148"
ORIGIN      
        1 mlskeppyap letakssdyf liqlpspqcc laivtecsdv iisllllsgd ietnpgpasl
       61 etvvtelkkl stgqstliae vtdlknqllt tdnlisdlsr risaleghyq tiqslrteie
      121 glsthttqat rqlcdledri deaenqsrrn nlifynipdp dpsetwadse kllirhcsef
      181 lditldpkti drthrlgrhe pdrcrpliak falfktkdei langrkfkna dysvgedfsr
      241 rvrnvrkhlv tfaksktnrf slryktlfig srryifdeps qsvkeip
//
LOCUS       XP_077495254             106 aa            linear   INV 25-NOV-2025
DEFINITION  histone-lysine N-methyltransferase PRDM7-like [Amblyomma
            americanum].
ACCESSION   XP_077495254
VERSION     XP_077495254.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639128.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..106
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..106
                     /product="histone-lysine N-methyltransferase PRDM7-like"
                     /calculated_mol_wt=11759
     Region          5..106
                     /region_name="PR-SET_PRDM-like"
                     /note="PR-SET domain found in PRDM (PRDI-BF1 and RIZ
                     homology domain) family of proteins; cd10534"
                     /db_xref="CDD:380932"
     Site            order(16..17,34,72..76,78,87..88)
                     /site_type="active"
                     /db_xref="CDD:380932"
     Site            order(16..17,72..76,78)
                     /site_type="other"
                     /note="SAM binding site"
                     /db_xref="CDD:380932"
     CDS             1..106
                     /gene="LOC144106324"
                     /coded_by="XM_077639128.1:1..321"
                     /db_xref="GeneID:144106324"
ORIGIN      
        1 mlefltsgks tvkgapvgvf tlkalskglh fgpyhlvkvh gietggctrp vhrcgeffvv
       61 egrpeernhw mndvnyspsk rtqnleaslv nsdiyyrtlr kvdpge
//
LOCUS       XP_077495652             205 aa            linear   INV 25-NOV-2025
DEFINITION  myb/SANT-like DNA-binding domain-containing protein 3 [Amblyomma
            americanum].
ACCESSION   XP_077495652
VERSION     XP_077495652.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639526.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 30% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..205
                     /product="myb/SANT-like DNA-binding domain-containing
                     protein 3"
                     /calculated_mol_wt=23077
     Region          8..85
                     /region_name="Myb_DNA-bind_5"
                     /note="Myb/SANT-like DNA-binding domain; pfam13873"
                     /db_xref="CDD:433544"
     CDS             1..205
                     /gene="LOC144106653"
                     /coded_by="XM_077639526.1:182..799"
                     /db_xref="GeneID:144106653"
ORIGIN      
        1 mektekgkin fsrveralll dlvdkhkavl enkrtdavsv arkrkeweli etqfnsshnv
       61 sprtwlqlkk cwenwknkwr kakaddnrei fktgggsaap sqltedlqrv gsvashmglr
      121 lanpfdsdrh qadpggesqp tpsvaallsr tqdgsipgas dhrdklekra avhqlemenl
      181 klkndvkrsk killelqika vkgel
//
LOCUS       XP_077495750             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106741 [Amblyomma americanum].
ACCESSION   XP_077495750
VERSION     XP_077495750.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639624.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144106741"
                     /calculated_mol_wt=20215
     CDS             1..181
                     /gene="LOC144106741"
                     /coded_by="XM_077639624.1:1..546"
                     /db_xref="GeneID:144106741"
ORIGIN      
        1 mlsvkkrlva askntgcgdl rhwvqpvvnh lywcvsvsag dgkllvaiwk smlnhvanvh
       61 tghgdpyprc lhdsipngkw lspgtpayar lvaitskrll lkdieklsas gqtynlesfh
      121 silnkfapks vsfstdimra ryilhktqlv ilhhnentcr aqavtkdger kwkrkqlrag
      181 k
//
LOCUS       XP_077495978             216 aa            linear   INV 25-NOV-2025
DEFINITION  zinc transporter Zip99C [Amblyomma americanum].
ACCESSION   XP_077495978
VERSION     XP_077495978.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639852.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..216
                     /product="zinc transporter Zip99C"
                     /calculated_mol_wt=22710
     Region          <61..211
                     /region_name="Zip"
                     /note="ZIP Zinc transporter; pfam02535"
                     /db_xref="CDD:396884"
     CDS             1..216
                     /gene="Zip99C"
                     /coded_by="XM_077639852.1:1..651"
                     /db_xref="GeneID:144106872"
ORIGIN      
        1 msgaesptqa hltlglwvlv gvftfivlel vftatspete qtfshsdvnq ngvtklvpal
       61 enlktihvtg ylnlmangid nfthglavaa sflvgpkmgv vttlailihe iphevgdfai
      121 llksgfnrwd aakaqvltaa vgvagavtal sadslenvdm stswilpfts ggflnialvs
      181 vlpdllkeed pwesskqlgc vccgiavmaa mqafle
//
LOCUS       XP_077496080             343 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106953 [Amblyomma americanum].
ACCESSION   XP_077496080
VERSION     XP_077496080.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639954.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..343
                     /product="uncharacterized protein LOC144106953"
                     /calculated_mol_wt=38609
     CDS             1..343
                     /gene="LOC144106953"
                     /coded_by="XM_077639954.1:13..1044"
                     /db_xref="GeneID:144106953"
ORIGIN      
        1 metpppatgi retarllqke khnyclpemp yhisrqlaqi sesaevssdl rraiirwlha
       61 dllsygpypg nlyaeaarrl vikhpvlsdk tgssfgswqi alkfrtkner rkitdveevn
      121 darkkarrsa ggcpivaska atkrgeipvn dtedehsiqa lkefmkketk kcvedqnipr
      181 lqdamrrtfa errrwmaqsn atvleviaey palatleavh eeftritkqn geenllsfin
      241 khgerllera rklksaaaii kahdeassra mseeekkycf asavlqlmpc fvkenpkrff
      301 sqeenckfps vvaagdpfql fpqvaavegl kvdtsvealt aka
//
LOCUS       XP_077496358             436 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: F-box and WD repeat domain-containing
            11-A-like [Amblyomma americanum].
ACCESSION   XP_077496358
VERSION     XP_077496358.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640232.1
KEYWORDS    RefSeq; corrected model; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio   :: 13% of CDS bases
            frameshifts :: corrected 2 indels
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..436
                     /product="LOW QUALITY PROTEIN: F-box and WD repeat
                     domain-containing 11-A-like"
                     /calculated_mol_wt=49802
     Region          89..125
                     /region_name="Beta-TrCP_D"
                     /note="D domain of beta-TrCP; cl26390"
                     /db_xref="CDD:474793"
     Region          125..175
                     /region_name="F-box_SF"
                     /note="F-box domain superfamily; cl45894"
                     /db_xref="CDD:459239"
     Site            order(137..141,144..145,148,159..161,163,166)
                     /site_type="other"
                     /note="F-box motif"
                     /db_xref="CDD:438852"
     Site            order(137,140,143..144,147..148,152,154..155,159..161,163)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438852"
     Region          251..>436
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cd00200"
                     /db_xref="CDD:238121"
     Region          253..288
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          294..328
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          333..370
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          377..410
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..436
                     /gene="LOC144107200"
                     /coded_by="XM_077640232.1:1..1311"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 2 bases in 2 codons"
                     /db_xref="GeneID:144107200"
ORIGIN      
        1 maqmprcwva epfvstrlrp vcmrvsflkl vgpvrhssda wvikesdqrk dnqshckatq
       61 lcestkqcsg xgsrhaasqa drerclvsfd twpeevqtqf veallarmsh cqhsristfl
      121 mlmlqrdfis llpkkgldhv aeailsylda rslcaaeavc rewrrvvaeg glwrklierr
      181 vstdplwrgl aerrglvqyl fkpppgephp dhmfyrrlyp riiqdiesie nnwrcgrhnl
      241 qrincrsenx kgvyclqydd tkivsglrdn tikiwdrasl qcvkvltght gavlclqydd
      301 kviisgsyhs tvrvwdvktg emvntlihqr eavlhlrfnn dmmvtcskdh siavwdmvtp
      361 reinlrrvlv ghraavnvvd fderyvvsas gdrtikvwgt pncefvrtln ghkrgiaclq
      421 yrdrlvvsgs sdntir
//
LOCUS       XP_077496742            1545 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107528 [Amblyomma americanum].
ACCESSION   XP_077496742
VERSION     XP_077496742.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640616.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1545
                     /product="uncharacterized protein LOC144107528"
                     /calculated_mol_wt=164790
     Region          13..>121
                     /region_name="DUF4603"
                     /note="Domain of unknown function (DUF4603); pfam15376"
                     /db_xref="CDD:464684"
     Region          <941..1413
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..1545
                     /gene="LOC144107528"
                     /coded_by="XM_077640616.1:333..4970"
                     /db_xref="GeneID:144107528"
ORIGIN      
        1 mkyikattvp qeihswfqge lesrgidpvy shtvmslllh pdqefgnrga aveclksate
       61 qkfglealvd elcrrlnsyl npeshaeaaa appveepvrr ksprelaqmy yaafpalens
      121 pqgqqaqqra pwdgrriiel hereqqhaes vnwellqeqq lrskldspsl laiwgrqast
      181 giethifdff nsvwalsged kvmlkvlsrt gcegkepvca erfvqdegts kgelhgsgas
      241 snaeplgaal akiiamlvek hncsmavfkd etlfwnwakn vsddpllqai siagpaifre
      301 arhlmrktrf pnagqgdcla tdfvpashws dkeplaenla nlalgemask psisggkdll
      361 amgdvttepa kdsddswcgg fdptrefdyl dfeqediasa egrlaecsgl wpntsvssrs
      421 esqmtqisgr tcsssslpgk nskeeatdnl sqfgafmked ssragtwnpt wledfiracp
      481 naevppifqk irdqmegaaa rkaaaahsea ygteskasss gsfadsslsk nkrkdpslqk
      541 ghsaghnsas psvqvdcgaa ewawkagsng dmttaapwsp irlgnkvfpe inssnieelr
      601 neleaeldti lrnlyelsqa wdtlfstcga sqgtlplqqp gaappnhpyd fvphgtlpnt
      661 rlpyaqaqaa fttafnqqlp lgprptgffm pnmhtrpetp rqnfpefnah lgafaygqpe
      721 snqymprysm pssvgaplhs slissnsgtg gfvssdaran lavpsaeqva hgatfsstpg
      781 rsrsytanls eashkistrs cppncsvevs tytnelppty vpnfnatmkv kpsslatpsl
      841 qhtpvyatnl pgagyssvst meaahtvppa girkiqmpqa htpqprtfel sntqsygfpq
      901 killpnrsma nnaqgstsda stseasnisg kpflnvfhsn atnelavpep slpgplqagt
      961 dasskslpnl lqstnlppgt slpdssvcat aasdtlshdt lttvlpknsp spptlpasvg
     1021 nlnfgtipwt lprpsshpsh etsprppawh qrgpvpwstq garagpvncp lpghvapvrv
     1081 pqlaanqppg ctlvsphlnc svavhvppvr asppsahrns tntlaspqfk splaghapav
     1141 rasspsvnrp palanpqmrl gprpvprqpw htswapswpa harwpmsswp plasanrnpp
     1201 ehvprplarp mhgsalspat wpplragpav vrqpitelts vcgivpspsf gqvaklpahr
     1261 ppiatstaga stpqtpvtds llgprpqlpp ppssgstsss cstapqpnvk paltnatssa
     1321 svtsttstnt sppstappqp tavhvasnva apisrysavp aprhaariaq grcfkagvap
     1381 gtktallnpv prlpprlask latrqpatps cvsnsnsskl cpeadkpvnq pckdtqmvpe
     1441 atdqqpandd pkecdvkaqt ktedceqntk gecspctaps vgpgsawrkt pnikglagap
     1501 ghnlqdgtgk qtetapappe knrqetaavs nthphptsgr rrkrr
//
LOCUS       XP_077497061             200 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107744 [Amblyomma americanum].
ACCESSION   XP_077497061
VERSION     XP_077497061.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640935.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 39% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..200
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..200
                     /product="uncharacterized protein LOC144107744"
                     /calculated_mol_wt=21966
     CDS             1..200
                     /gene="LOC144107744"
                     /coded_by="XM_077640935.1:1..603"
                     /db_xref="GeneID:144107744"
ORIGIN      
        1 mastdtavrt lpscavvpqq tklvdigdka alwnsgtkrn vepamlehig fklqkgtvdg
       61 sprkcprprf vpmtqeeikk mheeppyael ftlpgtilye slhapdrqlk tpplpvktth
      121 gdhtdnsctq cnqfydkyva lsplaasale letvspatsl wqfsrklrlt asniskvhtv
      181 qpvlrqvrgf iasrsqcsga
//
LOCUS       XP_077497683             130 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase NRDP1-like [Amblyomma americanum].
ACCESSION   XP_077497683
VERSION     XP_077497683.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641557.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..130
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..130
                     /product="E3 ubiquitin-protein ligase NRDP1-like"
                     /calculated_mol_wt=14363
     Region          <17..130
                     /region_name="USP8_interact"
                     /note="USP8 interacting; pfam08941"
                     /db_xref="CDD:430333"
     CDS             1..130
                     /gene="LOC144108253"
                     /coded_by="XM_077641557.1:1..393"
                     /db_xref="GeneID:144108253"
ORIGIN      
        1 mslptcgllv ldyyvwweak lqrahvtrvg smistpdaml qamvkralte sgcsqhilqv
       61 ltenahkrcs ppglstldth qmnrrhyeay vckripgkqa vvvaagenql lnygmvlepg
      121 lvmifshgve
//
LOCUS       XP_077498385             272 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109431 [Amblyomma americanum].
ACCESSION   XP_077498385
VERSION     XP_077498385.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642259.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..272
                     /product="uncharacterized protein LOC144109431"
                     /calculated_mol_wt=29438
     Region          10..124
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     CDS             1..272
                     /gene="LOC144109431"
                     /coded_by="XM_077642259.1:1..819"
                     /db_xref="GeneID:144109431"
ORIGIN      
        1 marcyqpqtg qgaaalvapq lnatrtcrll fpdssaaael aglhlaadli stippgpvav
       61 lcdsrpalqq lsrphdnvvt tsllraslqt ledaghqisl hwvpghsgie gndladalak
      121 sahtdgspvs favtqhdfar pfimqmvral hpdrriaayk pfrrlsdqlp mrdralllll
      181 rigctwthsr lyacykahsp acpscgdtgt lghlllacpt hesprrrles gywalglptt
      241 mehdllfpar sqllaqrnll sflgktglsi ll
//
LOCUS       XP_077498653            1497 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109702 [Amblyomma americanum].
ACCESSION   XP_077498653
VERSION     XP_077498653.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642527.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1497
                     /product="uncharacterized protein LOC144109702"
                     /calculated_mol_wt=165934
     Region          94..200
                     /region_name="Btz"
                     /note="CASC3/Barentsz eIF4AIII binding; smart01044"
                     /db_xref="CDD:214991"
     Region          <288..554
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          <588..672
                     /region_name="Btz"
                     /note="CASC3/Barentsz eIF4AIII binding; smart01044"
                     /db_xref="CDD:214991"
     Region          <930..995
                     /region_name="Btz"
                     /note="CASC3/Barentsz eIF4AIII binding; cl09653"
                     /db_xref="CDD:447763"
     Region          <1251..1335
                     /region_name="Btz"
                     /note="CASC3/Barentsz eIF4AIII binding; cl09653"
                     /db_xref="CDD:447763"
     CDS             1..1497
                     /gene="LOC144109702"
                     /coded_by="XM_077642527.1:1..4494"
                     /db_xref="GeneID:144109702"
ORIGIN      
        1 mtgekqvpwe asvsrsaaga gqeggheaaa dslespgnlg sdqeepddel elhpdvskdd
       61 lnensavndg ircledeled dldeeeeycl kgerqqgdge esgdpsdlqd kvldfdedkr
      121 npqyipkkga fyqhddrlvg dgdeseipee kneddkgsrr qkklwndegv wghdmyreee
      181 qgpktseelv siygydirte tmpprarrrr rygrgpnkyq rnwedeeayt prsgsgrggg
      241 mrggrrprgg gqfgnppfqd edfpdlnakh sesessailn qdqavvkvpt kpaafkspdh
      301 gapdnnspqq gewehvakss gtarrgwgkr psqpprrpad tspkqhedvv kenmssdhha
      361 skpapqsplk taesfhspps lgrsralrhv lteqersrrk aeinektvpk velpdggaad
      421 ltrnsaatva prggpiggte vagkprryss lrqrplaetp pyteatvaap qlpnaqappp
      481 gpsqpqqail taahfqgpya pypddymlpp aqapavmppp sqpppvmapp tqmsspflpp
      541 pggilsfppq ypppytfpaq yaapapaapp ptecgdpsdl qgkvlgided ksnpqcttkk
      601 gafhqhddrl vghgdeseip eerneddkgs rrqnklcrde gvwshdmyre deqgpktsee
      661 lvsiygydir tetmppraqs rrlyghgsnk yqrnwedeea htprsgsgrg rgiscdlrpr
      721 gggqfgnppf qdatlhqdqa vvkvptkpaa fkstdhgtpd khspqqvweh vakssgtarw
      781 dwgecpsqlp rkpadtspkq hedevkknms sdhhaskpap qsplktaess ynepaanaka
      841 ilqvllerrl srrnaemhek tvpddlalas satevrvawk agnqpdgtry tscgsrqqhp
      901 rssgkhgcit lewqesgdps dlqdkvlgid edksnpqcnp kkrafhqhdd rgsrhekklc
      961 rdegvwshdm yreeeqgpkt seelvsiygy dirtetmppr aqsrrlyghg snkyqrnwed
     1021 eeahtprsgs grgrgiscdf rprgggqfgn ppfqdatlhq dqavvkvptk paafkstdhg
     1081 tpdkhspqqv wehvakssgt arwdwgecps qpprkpadts pkqhedevkk nmssdhhask
     1141 papqsplkta essynepaan akailqvlle rrrsrrnaer dektvpkvel pnggaadltr
     1201 nsaatvaprg gpiggtevag kprrysslrq rplaeecgdp sdlqgkvlgi dedksnpqct
     1261 tkkgafhqhd drlvghgdes eipeernqdd kgsrrqnklc rdegvwshdm yreeeqgpkt
     1321 seelvsiygy dirtetmppr aqsrrlyghg snkyqrnwed eeahtprsgs grgrgiscdr
     1381 rprgggqfgn ppsqdatlhq dqavvkvptk paaskspyhg tpdnspqqge wehlakssgt
     1441 arwdwgecps qpprtpadts skqhedeail qvllerrrsr rnaerdektv pkvelpk
//
LOCUS       XP_077499362             106 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144110353 [Amblyomma americanum].
ACCESSION   XP_077499362
VERSION     XP_077499362.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643236.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..106
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..106
                     /product="uncharacterized protein LOC144110353"
                     /calculated_mol_wt=11108
     Region          12..79
                     /region_name="DM9"
                     /note="Repeats found in Drosophila proteins; smart00696"
                     /db_xref="CDD:128937"
     CDS             1..106
                     /gene="LOC144110353"
                     /coded_by="XM_077643236.1:1..321"
                     /db_xref="GeneID:144110353"
ORIGIN      
        1 mgavlasdsa smawlpssgg evssgaiqgg vtssgeplni grghhkgnlt igkfhpshkt
       61 vyvpfsgvey gysqyeilvh etinfreatk rltefrgtps hppggr
//
LOCUS       XP_077499476             106 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144110427 [Amblyomma americanum].
ACCESSION   XP_077499476
VERSION     XP_077499476.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643350.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..106
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..106
                     /product="uncharacterized protein LOC144110427"
                     /calculated_mol_wt=11108
     Region          12..79
                     /region_name="DM9"
                     /note="Repeats found in Drosophila proteins; smart00696"
                     /db_xref="CDD:128937"
     CDS             1..106
                     /gene="LOC144110427"
                     /coded_by="XM_077643350.1:1..321"
                     /db_xref="GeneID:144110427"
ORIGIN      
        1 mgavlasdsa smawlpssgg evssgaiqgg vtssgeplni grghhkgnlt igkfhpshkt
       61 vyvpfsgvey gysqyeilvh etinfreatk rltefrgtps hppggr
//
LOCUS       XP_077499606             532 aa            linear   INV 25-NOV-2025
DEFINITION  putative fatty acyl-CoA reductase CG5065 [Amblyomma americanum].
ACCESSION   XP_077499606
VERSION     XP_077499606.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643480.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..532
                     /product="putative fatty acyl-CoA reductase CG5065"
                     /calculated_mol_wt=59555
     Region          35..354
                     /region_name="FAR-N_SDR_e"
                     /note="fatty acyl CoA reductases (FARs), extended (e)
                     SDRs; cd05236"
                     /db_xref="CDD:187547"
     Site            order(41,43..46,68..70,135..137,154,175..177,228,232,
                     253..256)
                     /site_type="other"
                     /note="putative NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:187547"
     Site            order(155,177,228,232)
                     /site_type="active"
                     /db_xref="CDD:187547"
     Site            order(177,228,255,267,283,289)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:187547"
     Region          380..471
                     /region_name="Sterile"
                     /note="Male sterility protein; pfam03015"
                     /db_xref="CDD:460779"
     CDS             1..532
                     /gene="LOC144110492"
                     /coded_by="XM_077643480.1:1..1599"
                     /db_xref="GeneID:144110492"
ORIGIN      
        1 masslsgqql qpslptaavt qgsgdsqiah fyqdrvvfvt ggtgfigkvl lekllrscpg
       61 lkqvyllirs kreeqpqarl atilnsqvfe rlkqeqpaal ekvravsgdi tlpslglsrs
      121 dwatlveevs vvfhsaatik fdeplnvavq lnllgtrrvl elckqipnls alvhvstsys
      181 ncdqngelhe viyqphvqak klieavegqn dnyiattddi lcglpntytf tkrlaesllr
      241 dergaipvai vrpsivtasw repfpgwidn fasctglivs lglglspslm vrkdstidii
      301 pvdivantli cvawhtattr pehvavyhct sgafqrhtwg ewtevvqknv lryplpqavr
      361 ypkfevtgsp lwhsanhwcl hylpacagdl alrimgrepr faaryqkvrk fldtaqyftt
      421 hgwlfrsdnv vgligalspk dkklfrhdiq niewgpyweq hmlgivkylf kaedeklpda
      481 rrqlkrlyav hlslklllla lvspllmtqa awemgysmkt vvtglyemvf tl
//
LOCUS       XP_077499713             174 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144110545 [Amblyomma americanum].
ACCESSION   XP_077499713
VERSION     XP_077499713.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643587.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 35% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..174
                     /product="uncharacterized protein LOC144110545"
                     /calculated_mol_wt=18422
     Region          15..163
                     /region_name="DUF3421"
                     /note="Protein of unknown function (DUF3421); pfam11901"
                     /db_xref="CDD:463390"
     CDS             1..174
                     /gene="LOC144110545"
                     /coded_by="XM_077643587.1:1..525"
                     /db_xref="GeneID:144110545"
ORIGIN      
        1 mdtqqqapsa gwqgvggekm yvgrvelwgd llpgkvvpsh gacyascggn eiaytcagps
       61 haqfagehsp sdrvrlpgml pglqsgsqsn rhaikrtpva tgviaasqvl vtddyslvwr
      121 pasrgavppg avraehqgsl tvgkvhlshr clyipfggse hryteyevlv yhtc
//
LOCUS       XP_077499829             329 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144110629 [Amblyomma americanum].
ACCESSION   XP_077499829
VERSION     XP_077499829.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643703.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..329
                     /product="uncharacterized protein LOC144110629"
                     /calculated_mol_wt=36311
     Region          62..145
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..329
                     /gene="LOC144110629"
                     /coded_by="XM_077643703.1:1..990"
                     /db_xref="GeneID:144110629"
ORIGIN      
        1 maaplriile gvereifeav ddtgapivhg nqrlyatadn vqvwlnapaa pvphtiepee
       61 lwprakvlfl iqeyknfksd pskrlktkrq lwenladlvn skfgchlths qienkwrnle
      121 rkyktvrqhn gqsgadrrtc efeeelaeif ekahsinppy llgqglvrsv qdsqaepaas
      181 pqpgpsrvta taspipgssq pstsrgstta gdsqppspsa qpsplqqqpg parkrprtdt
      241 sravlesava hlevaeanrq qrhrermale grkvaaeerr taalervaaa lerrelvslc
      301 spihapahsp ihspirspvr spsppyyna
//
LOCUS       XP_077499930             163 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144110708 [Amblyomma americanum].
ACCESSION   XP_077499930
VERSION     XP_077499930.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643804.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..163
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..163
                     /product="uncharacterized protein LOC144110708"
                     /calculated_mol_wt=19114
     CDS             1..163
                     /gene="LOC144110708"
                     /coded_by="XM_077643804.1:93..584"
                     /db_xref="GeneID:144110708"
ORIGIN      
        1 magadddvlt tmsvlvivcs lllrrrraqk arrrrfwvhp cwryrdvegq anallprlra
       61 rdegffrdfl rmppssfdtl lhlvrpvier qdtpfrrsis ahdrlamtvr tingwndrkg
      121 nlccimgnts anlckistns rrmeknchrh acrvefsqlh wmy
//
LOCUS       XP_077500201             130 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144110965 [Amblyomma americanum].
ACCESSION   XP_077500201
VERSION     XP_077500201.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644075.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 26% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..130
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..130
                     /product="uncharacterized protein LOC144110965"
                     /calculated_mol_wt=13572
     CDS             1..130
                     /gene="LOC144110965"
                     /coded_by="XM_077644075.1:1..393"
                     /db_xref="GeneID:144110965"
ORIGIN      
        1 mgsddqlqrq aglqtrtsmm ctyaamplpe sassrtcplq qpgssllpns dsasmpeats
       61 lqqcpsgesr dstnatchav pkssatvpli mdalpkqslf eeaanlmlra satsseslgt
      121 cynllisedv
//
LOCUS       XP_077500427             117 aa            linear   INV 25-NOV-2025
DEFINITION  natterin-3-like, partial [Amblyomma americanum].
ACCESSION   XP_077500427
VERSION     XP_077500427.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644301.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         <1..117
                     /product="natterin-3-like"
     Region          <35..96
                     /region_name="DUF3421"
                     /note="Protein of unknown function (DUF3421); pfam11901"
                     /db_xref="CDD:463390"
     CDS             1..117
                     /gene="LOC144111121"
                     /coded_by="XM_077644301.1:<1..354"
                     /db_xref="GeneID:144111121"
ORIGIN      
        1 casfcernhs pseeraipte atwpatdalv segatlewlp gsdgsvpsga lpggktasge
       61 qlyigrarhe gmlipgkvhp sikcafipye gkeyryaeye vlvsktisfr eakerrd
//
LOCUS       XP_077500773             326 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111339 [Amblyomma americanum].
ACCESSION   XP_077500773
VERSION     XP_077500773.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644647.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..326
                     /product="uncharacterized protein LOC144111339"
                     /calculated_mol_wt=34629
     CDS             1..326
                     /gene="LOC144111339"
                     /coded_by="XM_077644647.1:385..1365"
                     /db_xref="GeneID:144111339"
ORIGIN      
        1 mgalvcrsre paepgatvlt dspelwakpa aasrgqagrl pwhrtrrakp sgpaavvspt
       61 gdncpcccag aaaaaaaklq pegtplerae sppnkisfsr sawetppspl ldktssvhee
      121 eplyveeatd lltdteqqcq fafsgsddmt qveeitesgg epctfctlyp dgtlattddd
      181 ddfkgaaeql calypgaarq paaagepcsl cllfpgdsga aprddapral ddgwsnldee
      241 vtimvdrvss lssiveeklr vknktrqdvw kgtfgiyvhh akdgtkkivi rdgwtsyhiq
      301 gdgtgylkpt iasmkgwgla sdsrdg
//
LOCUS       XP_077500878            1028 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111419 [Amblyomma americanum].
ACCESSION   XP_077500878
VERSION     XP_077500878.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644752.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1028
                     /product="uncharacterized protein LOC144111419"
                     /calculated_mol_wt=113004
     Region          <12..56
                     /region_name="EEP"
                     /note="Exonuclease-Endonuclease-Phosphatase (EEP) domain
                     superfamily; cl00490"
                     /db_xref="CDD:469791"
     Region          288..551
                     /region_name="RT_nLTR_like"
                     /note="RT_nLTR: Non-LTR (long terminal repeat)
                     retrotransposon and non-LTR retrovirus reverse
                     transcriptase (RT). This subfamily contains both non-LTR
                     retrotransposons and non-LTR retrovirus RTs. RTs catalyze
                     the conversion of single-stranded RNA into...; cd01650"
                     /db_xref="CDD:238827"
     Site            order(378..383,437..438,470,472..473,548..549)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238827"
     Site            order(378..383,437,472)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238827"
     Site            438
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238827"
     Region          753..880
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     Site            order(757..760,789..791,794,819,858,876)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260008"
     Region          <939..975
                     /region_name="zf-RVT"
                     /note="zinc-binding in reverse transcriptase; pfam13966"
                     /db_xref="CDD:433612"
     CDS             1..1028
                     /gene="LOC144111419"
                     /coded_by="XM_077644752.1:1..3087"
                     /db_xref="GeneID:144111419"
ORIGIN      
        1 meatlraglg llkppsptfv tlrhsstldl afttpgirye crkpadtwgs dhfplfltrt
       61 srrpqedrty aviswpefrv rskeippgqs fmdhvatcar eattlvrvpa sspvpdlrll
      121 niranrrrqe rialntslpa dwtayrrida acrrharrrr rhswtgvcss iqrnshsgka
      181 wrllkalvhp eiprrpilai aiaraisied laelmadqfn ppggpcyaai tahelqavld
      241 rprkrtapga dgishqmlrn ldtpertrll eafnaiwgsa tlpedwltav vvpvlkprks
      301 srlpssyrpv sltsaacktm eaialfrltw iarvtnflpe qltgfrrgrc tadsiadlvs
      361 tledarhdgd avmlvlldvk aafdtlphnv ihealsrlgv tgplmafiqa flqgrtfrvr
      421 vgrqlssprp vaagvpqgsv lspflfnlam aglpaslksr sshrvrcsvy addvalwvrg
      481 tprrirsmrt slqgaldaaq aylssigltv spakteally hprgrrahit tlrlgadgpt
      541 wrnqvkylgl ltdrrltwlp avrallprlr rigqavqrlq ardkgcspsw alrlyhaaat
      601 slvtyalplv tlpparlrtl elghrsvlrq clglprispi aathaeagsw plsllllqtg
      661 lrhvnrlyha pdgrallsrl rrapdsrmgl lystfhrlfg ppvnqapfpp pptrpplpis
      721 vdirscsrrr tpvaalqqta atplqeslgg hlqvytdgsv lpatgqaaaa cvapalgaal
      781 scrlrfpans taaelaglhl aadlllllpg qqpaviltds raalqllrqh qprqhtvanl
      841 tarlmaiqda grpvslqwlp shvgitgnea adqlagaaht ngspvsskvt qldfarpalr
      901 qavralhpdp rvasgvrfmr vpdclprrer ililrlrtgc swtqarlhrh grapspacsf
      961 cgadetlghl lcacpnleap rrdmtagyrn lglpsysdqd llhpersqte afrllleflq
     1021 stglatrl
//
LOCUS       XP_077500979             839 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111498 [Amblyomma americanum].
ACCESSION   XP_077500979
VERSION     XP_077500979.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644853.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 43% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..839
                     /product="uncharacterized protein LOC144111498"
                     /calculated_mol_wt=94191
     Region          144..471
                     /region_name="Zinc_peptidase_like"
                     /note="Zinc peptidases M18, M20, M28, and M42; cl14876"
                     /db_xref="CDD:472712"
     Region          702..>806
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     CDS             1..839
                     /gene="LOC144111498"
                     /coded_by="XM_077644853.1:21..2540"
                     /db_xref="GeneID:144111498"
ORIGIN      
        1 mgcwalllll lrlvgsiygc avptglthll kmpkrsvasp ivhspdpawp rsrqwrlpeg
       61 rsgrrpsqsf rttvvltdee yfaslhpaes qnspeaadqk pffdgypygr lrgadeaepa
      121 dersttdper dsetetlkss advlrdhleg flsdmrndle pslkerarfr ifnefldygl
      181 etryqnfsge lyrelmdhsq ptsgvniigv lsgkhrrtpd dhlivlgahy dtfkktkgvn
      241 dngsgiaall eaarvltahr cdldytvifv aldmeeigcv gsyyfvndfl itsellrdgs
      301 kfqgaiildt ilhyndtlys qdipedfqha splvaqaiva dgfrgnflas msrwrmderl
      361 arsfsrawhq qsgphfglhs laiplgpqpl aanlakhlnf lrsdhvmfwy hshpvykdsl
      421 sailltdtgp frgtmrecyh nvcddatalt dvnlqfvqkt tdslistivd lakgscdagv
      481 sdapiteaev waalqqlrtt sarspegitt kiattpssrf qrtadtratr pptskpelss
      541 agqrtkeaes yanllarrsw ddfsdqlndt lctektwrii kalldptktr sesnkaiqri
      601 ihtfegddne llkrieekvy stekpdaydk eyrgednphl drqitreevy aaakaatrnt
      661 aagadrirnf mirnlsdeav rqltqfineh wekgtvpeew khatvvmipk pgtklqienl
      721 rpisltsclg klferivtrr lqqhleekgh ypdsmsgfra klsaqdvllq ikeevltqvp
      781 yngesvimal dvkdafdnag haaimeglnn ancgkkihyy traslsnrtv tvgigelrg
//
LOCUS       XP_077500990             294 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111631 [Amblyomma americanum].
ACCESSION   XP_077500990
VERSION     XP_077500990.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644864.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..294
                     /product="uncharacterized protein LOC144111631"
                     /calculated_mol_wt=33169
     Region          65..146
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..294
                     /gene="LOC144111631"
                     /coded_by="XM_077644864.1:241..1125"
                     /db_xref="GeneID:144111631"
ORIGIN      
        1 mgeeaqrlfm amvnasplta tpaaidalas renieerpde ntagsdager glqpapgvkr
       61 gggapewtpr etklmldyyy kyfpqvgpfk kfknkkmlfk qvsqdlatvl gchktpqqce
      121 nryktvmrqk kkasdnnnks gaspcpvpfd demrkiqsid dslepevqrd sfgvtykpsa
      181 sssadssadp pspllessst atnssesgds tkgsaevkkr efrastsrmq qmqfffdqmr
      241 aisaermarr eerenekekr raerraertq erqerrkmhe dklqlireal glrq
//
LOCUS       XP_077501001             185 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111787 [Amblyomma americanum].
ACCESSION   XP_077501001
VERSION     XP_077501001.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644875.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..185
                     /product="uncharacterized protein LOC144111787"
                     /calculated_mol_wt=20298
     Region          39..59
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          67..87
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(72,74,76,78..79,82..83,86,100,102,106..107,110..111,
                     114,128,130,132,134..135,138..139,142)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          80..104
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          95..115
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          119..>180
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          123..143
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          151..170
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..185
                     /gene="LOC144111787"
                     /coded_by="XM_077644875.1:452..1009"
                     /db_xref="GeneID:144111787"
ORIGIN      
        1 mappvdpcfm gtlqpvpqav paaaaagppg ldagtgfwcd lcgkvfdrrq allshgcrsh
       61 gicrfvcthc gaafrqssql tkhnrthtge rpfpcqvcqr sfaqksnlve hvrthtgerp
      121 yrchicqatf srkahlvght rkhtgerpfq cpacpkafst ssalnkhrrv thrspendns
      181 prpll
//
LOCUS       XP_077501084             164 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111898 [Amblyomma americanum].
ACCESSION   XP_077501084
VERSION     XP_077501084.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644958.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..164
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..164
                     /product="uncharacterized protein LOC144111898"
                     /calculated_mol_wt=19134
     Region          23..43
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          51..71
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(56,58,60,62..63,66..67,70,84,86,90..91,94..95,98,
                     112,114,116,118..119,122..123,126)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          79..99
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          103..>164
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          107..127
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          135..152
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..164
                     /gene="LOC144111898"
                     /coded_by="XM_077644958.1:86..580"
                     /db_xref="GeneID:144111898"
ORIGIN      
        1 mwlccrwvsd prayvppypy fwcelcgkvf arrqavlshc rrshgilrfv csycgeafrq
       61 rvhltkhtrt htgerpfpcq mcqshftqkv hlvehmrtht gerpyrchic qatftqkahl
      121 vghtrthtge rpfqgpacpk afatssalnq hryvthrntk ndnn
//
LOCUS       XP_077501181             185 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111939 [Amblyomma americanum].
ACCESSION   XP_077501181
VERSION     XP_077501181.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645055.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..185
                     /product="uncharacterized protein LOC144111939"
                     /calculated_mol_wt=20271
     Region          39..59
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          67..87
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(72,74,76,78..79,82..83,86,100,102,106..107,110..111,
                     114,128,130,132,134..135,138..139,142)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          80..104
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          95..115
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          119..>180
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          123..143
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          151..170
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..185
                     /gene="LOC144111939"
                     /coded_by="XM_077645055.1:133..690"
                     /db_xref="GeneID:144111939"
ORIGIN      
        1 mappvdpcfm gtlqpvpqav paaaaagppg ldagtgfwcd lcgkvfdrrq allshgcrsh
       61 gicrfvcthc gaafrqssql tkhnrthtge rpfpcqvcqr sfaqksslve hvrthtgerp
      121 yrchicqatf srkahlvght rkhtgerpfq cpacpkafst ssalnkhrrv thrspendns
      181 prpll
//
LOCUS       XP_077501259             489 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112027 [Amblyomma americanum].
ACCESSION   XP_077501259
VERSION     XP_077501259.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645133.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 33% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..489
                     /product="uncharacterized protein LOC144112027"
                     /calculated_mol_wt=54131
     Region          39..60
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          <41..162
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          67..87
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(72,74,76,78..79,82..83,86,100,102,106..107,110..111,
                     114,128,130,132,134..135,138..139,142)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          95..115
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          119..485
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          123..143
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          151..172
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          252..269
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          343..364
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          371..391
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(376,378,380,382..383,386..387,390,404,406,410..411,
                     414..415,418,432,434,436,438..439,442..443,446)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          399..419
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          427..447
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          455..476
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..489
                     /gene="LOC144112027"
                     /coded_by="XM_077645133.1:133..1602"
                     /db_xref="GeneID:144112027"
ORIGIN      
        1 mappvdpcfm gtlqpvpqav paaaaagppg ldagtgfwcd lcgkvfdrrq allshgcrsh
       61 gicrfvcthc gaafrqssql tkhnrthtge rpfpcqvcqr sfaqksslve hvrthtgerp
      121 yrchicqatf srkahlvght rkhtgerpfq cpacpkafst ssalnkhrrv thrspendns
      181 pasnvskkkr wrqlleekkk kkknrnsrvl yavpylrtti qvderidaim rkatkraldf
      241 pvatsnaklk acdicqatlt qkahlvghtr thtgerpfqg pacpkasqgl rpampaippa
      301 ppqfmaprst lvswgprsrl pqavpaaaaa dppgldagtg fwcdlcgkvf drrqallshg
      361 crshgicrfv cthcgaafrq ssqltkhnrt htgerpfpcq vcqrsfaqks nlvehvrtht
      421 gerpyrchic qatfsrkanl vghtrthtge rpfqcpacpk afstssalnq hrrvthrspe
      481 kdnsprpll
//
LOCUS       XP_077501302             192 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112121 [Amblyomma americanum].
ACCESSION   XP_077501302
VERSION     XP_077501302.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645176.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..192
                     /product="uncharacterized protein LOC144112121"
                     /calculated_mol_wt=21049
     CDS             1..192
                     /gene="LOC144112121"
                     /coded_by="XM_077645176.1:1..579"
                     /db_xref="GeneID:144112121"
ORIGIN      
        1 mverlhpklm aalvarldre hwvdrlsmgl lglcavlrrd lgclaaelvf gaplrlsrdl
       61 fvpspplsqp lqylqhvqdc ihhlrpvppr ssdrhvfmhp dvasstrrll ppsyngpyrv
      121 lnktkklati llngceevvs ldglkpayle tppqvlpldv aslsadqpaa kaapslphrr
      181 vhfavpsgrg al
//
LOCUS       XP_077501314             572 aa            linear   INV 25-NOV-2025
DEFINITION  beta-scruin-like [Amblyomma americanum].
ACCESSION   XP_077501314
VERSION     XP_077501314.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645188.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..572
                     /product="beta-scruin-like"
                     /calculated_mol_wt=62677
     Region          20..318
                     /region_name="NanM"
                     /note="N-acetylneuraminic acid mutarotase [Cell
                     wall/membrane/envelope biogenesis]; COG3055"
                     /db_xref="CDD:442289"
     Region          80..132
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          136..181
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          184..233
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          235..283
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          286..331
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     CDS             1..572
                     /gene="LOC144112276"
                     /coded_by="XM_077645188.1:14..1732"
                     /db_xref="GeneID:144112276"
ORIGIN      
        1 mtprtvdern eilgsffdvs fygdlatalq ltyngpcrnl mliggisptk laddahgaam
       61 lsygpghdrw rpygfmaepr cdrtvvivrn ndvmlaggld plcvttsghm qpsnkaflfs
      121 srwkrrielp amsrerafha avgwhgrmmv fggfhrtgrm lssaevyffn tnqsllirpm
      181 smalmrmaga pidtrvyiig gtscgpdgha lqasiyifds stdswtklaa mmekraffsa
      241 lavqrdcwla ggvmqlrpkv kcttcidvlr lasstmqrrs vmpsprhsvc aektgcevyl
      301 fggqdennrp lgdvimldsa rrsfmqcepl prilagsata vmpedgldwl rlrsiwpgkk
      361 atpnireraa tviqkayrny rrgerlqprl kntwpppars tetleshwda ngaeasgatq
      421 ylrqqtlren mdpnlgmvgr mdgsflktnk tatappdvla ptthptpqvh pvllsspahp
      481 aslvpaalka spadraplai kappappalp alptstahpt lppppalppp pahpahaadq
      541 epsvlpapqd sppppalrrs qrirrkpdrw gy
//
LOCUS       XP_077501376             513 aa            linear   INV 25-NOV-2025
DEFINITION  beta-scruin-like [Amblyomma americanum].
ACCESSION   XP_077501376
VERSION     XP_077501376.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645250.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..513
                     /product="beta-scruin-like"
                     /calculated_mol_wt=56609
     Region          4..260
                     /region_name="NanM"
                     /note="N-acetylneuraminic acid mutarotase [Cell
                     wall/membrane/envelope biogenesis]; COG3055"
                     /db_xref="CDD:442289"
     Region          21..73
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          77..122
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          125..174
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     CDS             1..513
                     /gene="LOC144112382"
                     /coded_by="XM_077645250.1:1..1542"
                     /db_xref="GeneID:144112382"
ORIGIN      
        1 mlfygpghdr wrpygfmaep rcdrtvvivr nndvmlaggl dplcvttsgh mqpsnkaflf
       61 ssrwkrriel pamsrerafh aavgwhgrmm vfggfhrtgr mlssaevyff ntnqsllirp
      121 msmalmgmaa apidtrvyii ggtscgpdgh alqasiyifd pstdswtkla ammekraffs
      181 altvqrdwwl aggvmqlrpe vkcttcfdvl rlasstmerr yvmpsprhsv caektgyevy
      241 lfggqdennr plgdvimfdr arrsfmqcep lprilagsat avmpedgldw lrlrsiwpgk
      301 katpnirera atviqkayrn yrrgerlqpr lkntwpppar stetleshwd angaeasgat
      361 qylrqqtlpe nmdpnlgmvg rmdgsflktn ktatappdvl aptthptpqv hpvllsspah
      421 paslvpaalk aspadtapqa ikappsppal palpkstahp tlpappalpp ppahpahaad
      481 qepsvlpapp dsppppalrr sqrirrkpdr wgy
//
LOCUS       XP_077501446             263 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112468 [Amblyomma americanum].
ACCESSION   XP_077501446
VERSION     XP_077501446.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645320.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 11% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..263
                     /product="uncharacterized protein LOC144112468"
                     /calculated_mol_wt=27622
     Region          <16..>174
                     /region_name="PRK12323"
                     /note="DNA polymerase III subunit gamma/tau; cl46901"
                     /db_xref="CDD:481241"
     Region          239..>256
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     CDS             1..263
                     /gene="LOC144112468"
                     /coded_by="XM_077645320.1:1..792"
                     /db_xref="GeneID:144112468"
ORIGIN      
        1 melpimtvkq qvvglaeera spgpppacdr qapcppastv epalcfapaa pwtlpervre
       61 plsvqvcneg rwtpweavlf prraelplaa agrdgaaerp rsgsavraer rrtspsatas
      121 agehgaetld lspaasgagr krdvngamav araagqpppa sspvyagrfs vasplqdvpk
      181 dgltdfipkk slrrsvanes prswmdpklm lsaspdtnfs pkisgipcva aasrytapvh
      241 idvggtiyts sletltnler ecv
//
LOCUS       XP_077501509             315 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112558 [Amblyomma americanum].
ACCESSION   XP_077501509
VERSION     XP_077501509.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645383.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..315
                     /product="uncharacterized protein LOC144112558"
                     /calculated_mol_wt=36117
     Region          4..58
                     /region_name="PHD_SF"
                     /note="PHD finger superfamily; cl22851"
                     /db_xref="CDD:473978"
     Site            order(17..20,24,51)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:276966"
     Region          82..>157
                     /region_name="COG6"
                     /note="Conserved oligomeric complex COG6; cl19764"
                     /db_xref="CDD:473207"
     CDS             1..315
                     /gene="LOC144112558"
                     /coded_by="XM_077645383.1:1..948"
                     /db_xref="GeneID:144112558"
ORIGIN      
        1 mdtcfsckvs ltddkvfltc aeckscyhig tcsgvaenay kkmsqatkka wkcptckatk
       61 arcssltsdn teatvdlaqe iaeirrmlae vltikeklet letikitvdd ieksvelmst
      121 kydeviarme kqsadisdlg krvekletkl kegeikqlkr evnnleqysr rqnleihglq
      181 khnnenllek lnllandldl pplsesdvea ihrlptrkde nnvdkvapvl vrfasrvvrd
      241 kwlakkkelr dadskiflne nltthnkkll wlmktkatek eykfawvkng klfvrrnard
      301 kiinittied lelir
//
LOCUS       XP_077501579             275 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112641 [Amblyomma americanum].
ACCESSION   XP_077501579
VERSION     XP_077501579.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645453.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..275
                     /product="uncharacterized protein LOC144112641"
                     /calculated_mol_wt=30844
     Region          80..240
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..275
                     /gene="LOC144112641"
                     /coded_by="XM_077645453.1:234..1061"
                     /db_xref="GeneID:144112641"
ORIGIN      
        1 mpfedqillt lmrlrlglll ndlslrfgas taaisrifcs ivtklakfar eflvfwlprt
       61 airrtlpyvf eenysvtciv dcfeifidrp gqlhrrntty ssyksqntak ilqaiapsgf
      121 imflsklygg rasdrfitld sgfldhltqg deiladrgfn iddllppsvk vslpsfskgr
      181 pqicrsdvvs srrlarlrin versirrlkc frilkqfpas iftkkpland vlvaiaglcn
      241 lqpnlirdhg dasepgaqsp shpgkqvevv dntpe
//
LOCUS       XP_077501589             371 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112651 [Amblyomma americanum].
ACCESSION   XP_077501589
VERSION     XP_077501589.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645463.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..371
                     /product="uncharacterized protein LOC144112651"
                     /calculated_mol_wt=38761
     Region          30..113
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     CDS             1..371
                     /gene="LOC144112651"
                     /coded_by="XM_077645463.1:81..1196"
                     /db_xref="GeneID:144112651"
ORIGIN      
        1 mgsqqfclkw nnhqsnmlav fyqmlsneal vdvtlacegl slkahkmvls acspffqalf
       61 aenpckhpiv ilkdmryvdl raivefmyrg evvvsqedlm allktaetlk vkglvtsvdg
      121 aadsgpvgsp raespplskr krsrrprrrs psdsnksdse dappatrikv pdspeiiedg
      181 smssdhmtdp htaspasras nvasstavps garhgssvqa sisqhtqdsi gddvgegdda
      241 dfdvepldvm eesattesvp vfpdvasssq algdesqqsh htgstsgnsa lvpaqpslps
      301 dipitsqvdi epsptsltpy ddktiapvva aspaaassgd gastsmaatd aygvpaiagp
      361 sgyqqatpsh g
//
LOCUS       XP_077501620             371 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112675 [Amblyomma americanum].
ACCESSION   XP_077501620
VERSION     XP_077501620.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645494.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..371
                     /product="uncharacterized protein LOC144112675"
                     /calculated_mol_wt=38743
     Region          30..113
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     CDS             1..371
                     /gene="LOC144112675"
                     /coded_by="XM_077645494.1:81..1196"
                     /db_xref="GeneID:144112675"
ORIGIN      
        1 mgsqqfclkw nnhqsnmlav fyqmlsneal vdvtlacegl slkahkmvls acspffqalf
       61 aenpckhpiv ilkdmryvdl raivefmyrg evvvsqedlm allktaetlk vkglvtsvdg
      121 aadsgpvgsp raespplskr krsrrprrrs psdsnksdse dappatrikv pdspeiiedg
      181 smssdhmtdp htaspasras nvasstavps garhgssvqa sisqhtqdsi gddvgegdda
      241 dfdvepldvm eesattesvp vipdvasssq algdesqqsh htgstsgnsa ivpaqpslps
      301 dipitsqvdi epsptsltpy ddktiapvva aspaaassgd gastsmaatd aygvpaiagp
      361 sgyqqstpsh g
//
LOCUS       XP_077501629             214 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112681 [Amblyomma americanum].
ACCESSION   XP_077501629
VERSION     XP_077501629.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645503.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 37% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..214
                     /product="uncharacterized protein LOC144112681"
                     /calculated_mol_wt=23886
     Region          67..87
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          95..115
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(100,102,104,106..107,110..111,114,128,130,134..135,
                     138..139,142,156,158,160,162..163,166..167,170)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          107..132
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          123..143
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          147..>210
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          151..171
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          179..200
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..214
                     /gene="LOC144112681"
                     /coded_by="XM_077645503.1:1..645"
                     /db_xref="GeneID:144112681"
ORIGIN      
        1 mwlccrwvsd prayipanph yhqphhqvkt ppveprfvgp pkpepqevpa aaaagppgld
       61 agtgfscdlc gkvfvsrqal lshgrrshgi rrfvcthcga afqqsghlsk htrthtgerp
      121 fpcqicqsrf tqkvhlvehm rthtgerpyr chicqatfsr kahlvghtrt htgerpfqcp
      181 dcpkafatss avnqhrrvth rspendndsa rpll
//
LOCUS       XP_077501642             277 aa            linear   INV 25-NOV-2025
DEFINITION  palmitoyltransferase ZDHHC20-B-like [Amblyomma americanum].
ACCESSION   XP_077501642
VERSION     XP_077501642.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645516.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..277
                     /product="palmitoyltransferase ZDHHC20-B-like"
                     /calculated_mol_wt=30225
     Region          <19..116
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; pfam01529"
                     /db_xref="CDD:396215"
     CDS             1..277
                     /gene="LOC144112687"
                     /coded_by="XM_077645516.1:1..834"
                     /db_xref="GeneID:144112687"
ORIGIN      
        1 mkvrsgapgr nvynggpscv pkmdhhcpwf nncvcfstyk fflltifcvv alcvfglasa
       61 thhvagawss ntstyvtlnc tflyafgvml vlvlgsflyl hftmvcnnvt tledlrphlf
      121 kddrdsfdig frdniaevfg hrkalwflpv ftslgdgarf ptrlhpdhyv ygppvsvvve
      181 errpsvpvpp lpvadevpel pvltscrrpv pstegplggl tltpfvappr gslsflpptt
      241 itvvppteaa asvrlseskk dlppivvasa ftsmevr
//
LOCUS       XP_077501678             317 aa            linear   INV 25-NOV-2025
DEFINITION  flap structure-specific endonuclease 1 [Amblyomma americanum].
ACCESSION   XP_077501678
VERSION     XP_077501678.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645552.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..317
                     /product="flap structure-specific endonuclease 1"
                     /calculated_mol_wt=35878
     Region          1..316
                     /region_name="PIN_SF"
                     /note="PIN (PilT N terminus) domain: Superfamily; cl28905"
                     /db_xref="CDD:475124"
     Site            order(7,34,36..37,40,42,44..48,53..54,61,65,69..70,73,86,
                     93,100,128,133,158,160,179,181,192,195,314..316)
                     /site_type="active"
                     /db_xref="CDD:350215"
     Site            order(28..30,80,167..169,208,210..211,297..298,300)
                     /site_type="other"
                     /note="FEN1-PCNA interface [polypeptide binding]"
                     /db_xref="CDD:350215"
     Site            order(92..97,132..135)
                     /site_type="other"
                     /note="helical arch"
                     /db_xref="CDD:350215"
     CDS             1..317
                     /gene="Fen1"
                     /coded_by="XM_077645552.1:177..1130"
                     /db_xref="GeneID:144112718"
ORIGIN      
        1 mgitglakvi adnapdaike seiknyfgrk iaidasmcly qfliavrqen nmltnseget
       61 tshlvwffyr tirmiengik pvyvfdgkpp gmksselekr qerreaaeke lekaeeagnq
      121 eevnkfskrl akvtkqhsed ckkllslmgv pfieapceae aqcaelvkgg kvygtatedm
      181 dgltfgtnvl lrhmtysear kmpikefsle kvlsglemnr nefidlcill gcdycesirg
      241 igpkraleli kqhkciekii ssidtkkypv pedwpykear qlflepevta adevqlkwgd
      301 pdeeglvkfl ceengfr
//
LOCUS       XP_077501688             313 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112735 [Amblyomma americanum].
ACCESSION   XP_077501688
VERSION     XP_077501688.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645562.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..313
                     /product="uncharacterized protein LOC144112735"
                     /calculated_mol_wt=33769
     CDS             1..313
                     /gene="LOC144112735"
                     /coded_by="XM_077645562.1:86..1027"
                     /db_xref="GeneID:144112735"
ORIGIN      
        1 msssgapsya yvkyfdgaka ivhlslirey tpsslsdlak kkdvywgggk eeeegfysaq
       61 vmelgvtkad lirrlarrki pvpdfdfees epvatvsqen avsakdaann vktakrqklq
      121 elmdgsssdd dvlpkkllee rrknatllrr lkqvrkekae lqarhdried iflnkfeswl
      181 aarchlclke hssctcrter sgasplqqvr eclpyselta plaaattlqp splgdfknme
      241 askstglvmp qnlgalaess gqastsmapg plqnhpggvl gvsgqastsm apgplqnhpg
      301 gvlgvsgqan isq
//
LOCUS       XP_077501695             161 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112738 [Amblyomma americanum].
ACCESSION   XP_077501695
VERSION     XP_077501695.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645569.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 12% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..161
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..161
                     /product="uncharacterized protein LOC144112738"
                     /calculated_mol_wt=17269
     CDS             1..161
                     /gene="LOC144112738"
                     /coded_by="XM_077645569.1:190..675"
                     /db_xref="GeneID:144112738"
ORIGIN      
        1 mlgqpkqksk eedarekrdr hktgggsaqc tvraqseqvi avashimtrv gnqtdsdggi
       61 dlppvaslpv irllqpmvdg agneefyyae ddwepplvde pqspaaavgk sgatpeaieq
      121 esnlffpavq padasstaaa gcsrlqqcys cfwscrwahy c
//
LOCUS       XP_077501699             830 aa            linear   INV 25-NOV-2025
DEFINITION  serine_rich_CAS and FAT-like_CAS_C domain-containing protein
            p130CAS [Amblyomma americanum].
ACCESSION   XP_077501699
VERSION     XP_077501699.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645573.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..830
                     /product="serine_rich_CAS and FAT-like_CAS_C
                     domain-containing protein p130CAS"
                     /calculated_mol_wt=89939
     Region          17..72
                     /region_name="SH3_CAS"
                     /note="Src homology 3 domain of CAS (Crk-Associated
                     Substrate) scaffolding proteins; cd11844"
                     /db_xref="CDD:212778"
     Site            order(22,24,27,31,52..53,66,68..69)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212778"
     Region          455..611
                     /region_name="Serine_rich_CAS"
                     /note="Serine rich Four helix bundle domain of CAS
                     (Crk-Associated Substrate) scaffolding proteins; a protein
                     interaction module; cd11549"
                     /db_xref="CDD:211403"
     Region          630..825
                     /region_name="CAS_C"
                     /note="Crk-Associated Substrate C-terminal domain;
                     pfam12026"
                     /db_xref="CDD:463437"
     CDS             1..830
                     /gene="p130CAS"
                     /coded_by="XM_077645573.1:1..2493"
                     /db_xref="GeneID:144112750"
ORIGIN      
        1 maavarelwv vasqnclara lydniaespe elafrkndvl tvleqnpgdl egwwlcalrg
       61 rqglapgnrl rlmpgmydpt glgyaapaaa astdlgavpp agtypgpgqr harrrswrvd
      121 pnkvvtpskv gdvylydvpv gakvteeydt pahstlprtt ghvhidtivp apqegrkpvr
      181 alpnmaydep rpsslsydmp rpslnvrlel psstvsgtes ydvprpkady dvpnsepvgt
      241 ydtpppargv akgasssvyd vpldtgtprc vlaasydvpt sgrssgvsll stssssagvs
      301 passqsslnt slslsslcgs nrssleqghe mydiptsssr rvadappsrt qsprgnlsav
      361 ppppghgmtl lqfydvptnn apapfydtpp kraptaqavc megvydvppq vtrdaplpqp
      421 ltdetdasrr hsssssdsrg srdyetvgdl kelhlerdaa levlvkvqqe ltsaigklfs
      481 lthstlkrqe sgtaetrlyd iktschklrt slreflnfsq galanashve drklyqklar
      541 ltkplldsyq vvvattqtld ekgwqlcsms reggpdeldq liacarglte dvqqvastiq
      601 gnstlifkks plisesgrpa qerplplppk keddqwasdy dyvnleskas vereneeika
      661 alpaelsqsf dkllqqsqli vegivqepqa lsgapgldqn dlqilnfyga qidthilflt
      721 naidaflati ennqppkvfi ghskfvvisa hklvymgdtv yrnivnvdik nqvlqcssnl
      781 cealkvlins tkkaalqfps vaavqemvds ivdvshland lkvvvmqakr
//
LOCUS       XP_077501713             316 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: cytochrome c1, heme protein,
            mitochondrial-like, partial [Amblyomma americanum].
ACCESSION   XP_077501713
VERSION     XP_077501713.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645587.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         <1..316
                     /product="LOW QUALITY PROTEIN: cytochrome c1, heme
                     protein, mitochondrial-like"
     Region          84..301
                     /region_name="Cytochrom_C1"
                     /note="Cytochrome C1 family; pfam02167"
                     /db_xref="CDD:426633"
     CDS             1..316
                     /gene="LOC144112764"
                     /coded_by="XM_077645587.1:<1..951"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:144112764"
ORIGIN      
        1 tssavphqng csrgrfgnas flrlqsgsli skthastytp lsrgkkvffs tvgvvgagva
       61 gviyalnesv kasdlvlhpp klpwphketl issldhasir rgyevykqvc aachslrfia
      121 yrnlvgvsht eaeakaeaes vqitdgpddq gnmyqrpgkl sdyfpspypn deaaraanng
      181 alppdlsfit narhggedyl fhlltgycdp pagvkmqegq yynpyfpgga igmaqalyne
      241 aieysdgtpa tasqmakdvs tflkyctepe fderkrmfik ammilvalta vtwyykrfkw
      301 tvmktrkivy qpkkye
//
LOCUS       XP_077501745             258 aa            linear   INV 25-NOV-2025
DEFINITION  inactive selenide, water dikinase-like protein [Amblyomma
            americanum].
ACCESSION   XP_077501745
VERSION     XP_077501745.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645619.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..258
                     /product="inactive selenide, water dikinase-like protein"
                     /calculated_mol_wt=28322
     Region          <15..228
                     /region_name="PurM-like"
                     /note="AIR (aminoimidazole ribonucleotide) synthase
                     related protein. This family includes Hydrogen
                     expression/formation protein HypE, AIR synthases, FGAM
                     (formylglycinamidine ribonucleotide) synthase and
                     Selenophosphate synthetase (SelD). The N-terminal
                     domain...; cl10019"
                     /db_xref="CDD:471963"
     CDS             1..258
                     /gene="LOC144112802"
                     /coded_by="XM_077645619.1:1..777"
                     /db_xref="GeneID:144112802"
ORIGIN      
        1 mlreirvges nretgktaca nvlsglyalg vtrcdnmlmv maastkltek pllikgikwg
       61 pplwpdnaqp gdvlvltkpl gtqvavnahq wleklerraq ldkagiseed vrrvyvramd
      121 cmarlnlnar lmhkhgchga tdvtgfsllg haqnlvqvqq rsvgfvihtl piiakvpall
      181 patgtsfklm hghsaetsgg llmclpkena eayckeieqq egdpawiied vegewvarlm
      241 dsprmlevps adkesklw
//
LOCUS       XP_077501760             366 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112816 [Amblyomma americanum].
ACCESSION   XP_077501760
VERSION     XP_077501760.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645634.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..366
                     /product="uncharacterized protein LOC144112816"
                     /calculated_mol_wt=40616
     Region          <165..220
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..366
                     /gene="LOC144112816"
                     /coded_by="XM_077645634.1:51..1151"
                     /db_xref="GeneID:144112816"
ORIGIN      
        1 mykraldlpi ttsnqrlmgl gmvntfaelr eahltnqytr lsktpsgrrl larlhihhpt
       61 lteervripe iwryalhvrp lpanmtrddh sgrrlaraea larhygnkhd ilyvdasgph
      121 hggwytaavv hqkivvnglt fraqdithae eiaialaaad kesrvivtds rgacrnikqg
      181 hiplfahril qnsnylgapa srtivwapah mglegnetad aaaraltlra tpsdpldtdp
      241 epnpaltfre itelfqfgha iyhkpckglt kveertilrl ytktllcpav lkhfdpactg
      301 ecphcaekss difhmvwacq stpnlapkpn ptredweaal lgcsdlasqk vlvhrarvaa
      361 vangfl
//
LOCUS       XP_077501768             171 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112826 [Amblyomma americanum].
ACCESSION   XP_077501768
VERSION     XP_077501768.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645642.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 26% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..171
                     /product="uncharacterized protein LOC144112826"
                     /calculated_mol_wt=18786
     Region          <55..>90
                     /region_name="PRK09752"
                     /note="AIDA-I family autotransporter YfaL"
                     /db_xref="CDD:182059"
     CDS             1..171
                     /gene="LOC144112826"
                     /coded_by="XM_077645642.1:1..516"
                     /db_xref="GeneID:144112826"
ORIGIN      
        1 meafcdgsvd yvdenfcevd vedglgvarg evkdvvpdrg delgvsascr qntrsptdpt
       61 pvpspqpsqq htpepplqps tratpsllgk rrrddgasdv lrqmlyeshr ldsltdtrnr
      121 qdaafqqsml eaiwevtdav qasngqqell irnvnlltli lqkqleppap h
//
LOCUS       XP_077501903             431 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112937 [Amblyomma americanum].
ACCESSION   XP_077501903
VERSION     XP_077501903.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645777.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 42% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..431
                     /product="uncharacterized protein LOC144112937"
                     /calculated_mol_wt=47327
     Region          64..179
                     /region_name="V-set"
                     /note="Immunoglobulin V-set domain; pfam07686"
                     /db_xref="CDD:462230"
     Region          181..269
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; smart00060"
                     /db_xref="CDD:214495"
     Site            order(182,252,267)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     CDS             1..431
                     /gene="LOC144112937"
                     /coded_by="XM_077645777.1:1..1296"
                     /db_xref="GeneID:144112937"
ORIGIN      
        1 msalvtrvvr eyagavgahv ragfavsspp aavtrrqfgs rrseycrrtf fdcppphprr
       61 slhepvslav hvgsqidlpc ksllandlpl rinwfkddsp mpvysvtyas nqsfhrtnim
      121 qgrhaarsdw sqrayfsvlg spatlkvndl sfqdtgtyvc qvvisrgtqr natvhlvvve
      181 ppeplhncqi cnltetslgv tcwtghgspd aahgsaglnt tyvlelyssq qasqvlnvsg
      241 sqpvfsvrnl qprteyfivl ygvnslghsn avklyastlt saealldteh awnvlglgvy
      301 ssilivvlvi lclvllvlaf vvnrwrkkht lreagdrkkt clatidrnat vrketydade
      361 eevsvldikg lptaasapfg asidegasyv retasiagrl pchttdmyyt lpqlhsrhng
      421 irlqtfsrev m
//
LOCUS       XP_077501927             870 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112953 [Amblyomma americanum].
ACCESSION   XP_077501927
VERSION     XP_077501927.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645801.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 43% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..870
                     /product="uncharacterized protein LOC144112953"
                     /calculated_mol_wt=97273
     Region          115..200
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     Region          <272..319
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     Region          345..437
                     /region_name="Asp_protease_2"
                     /note="Aspartyl protease; pfam13650"
                     /db_xref="CDD:433378"
     Site            order(359,361,363,386..388,432)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133137"
     Site            359..361
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133137"
     Site            359
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133137"
     Site            order(386..389,393..396)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133137"
     Region          664..839
                     /region_name="RT_LTR"
                     /note="Reverse transcriptases (RTs) from retrotransposons
                     and retroviruses which have long terminal repeats (LTRs)
                     in their DNA copies but not in their RNA template. RT
                     catalyzes DNA replication from an RNA template, and is
                     responsible for the replication of...; cd01647"
                     /db_xref="CDD:238825"
     Site            order(727..732,761..762,789,791..792,836..837)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238825"
     Site            order(727..732,761,791)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238825"
     Site            762
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238825"
     CDS             1..870
                     /gene="LOC144112953"
                     /coded_by="XM_077645801.1:1..2613"
                     /db_xref="GeneID:144112953"
ORIGIN      
        1 msktpsgata gttdgqdggk vsdsisvsdf lsamrekdrl lasllerlal passgapgva
       61 aatttyqvmp dlsknisdfd gsgdavsake wlgnlqttat lhrwpepfkl etakchlvgp
      121 akdwlrsrke vstswenfdy hfrrtfcgqt raaerwrkmq ervqqrnest tayfhskvrl
      181 creanldfcd treqvltglr srqlctmllg rtheddddll hdimeferid rereqrfggm
      241 rekrqftaat ervaetppar kttydrfgkd rrppltnerg qfkcyncgrf ghiscdcpee
      301 rhemkclrcg ksghtqrhcs msqsnetntv tdterkiapg allkqvmlng tttlvgmidn
      361 gssgcllras aaarcgarlt kdatalygfg sqgtpsarai gkcytdleid gvkgengpfl
      421 vvpddtltvd llvgrtytel pyvayarlgg clrfwhksvc pfshleplne prlrvkatre
      481 tvihsnsinw itlssdsdls gpvlfctlgs eilvdmrege isvpiftsgd kdllvkrgrr
      541 lgrvmevdvv eipdsqkqpa rvsesdatep kvfcvaterr piedqqlevg psvtkqqkae
      601 llgllnkyrd cfanniselg ctpvlkmdit etpgsspvav rpcrtnaaer etireivgew
      661 kredivsets spyaspvilv qkkngekrlv vdyrrlnaqr lkekyplpsm deqleklsga
      721 klytvldlah gylqvplsde ansktafitp detgqfermt fgltnapsvf qrlmnrvlgp
      781 lrgtiamcyl ddvliparnw nellvrlrqv leafkranlt lrlskcafak dsveflgfrl
      841 kegtvqpgea kvqaielfpv pknahdvrrf
//
LOCUS       XP_077501936             167 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112963 [Amblyomma americanum].
ACCESSION   XP_077501936
VERSION     XP_077501936.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645810.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..167
                     /product="uncharacterized protein LOC144112963"
                     /calculated_mol_wt=18926
     Region          1..81
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            2
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238180"
     CDS             1..167
                     /gene="LOC144112963"
                     /coded_by="XM_077645810.1:1..504"
                     /db_xref="GeneID:144112963"
ORIGIN      
        1 mgasisvtma nltiediesr slatfnpkpk lflryvnncf cvvktseven llihlnstep
       61 aiqftvereq qgslpfldvl vtrhdnglrf svyrkpthtg rylhfnsnhp tpqkasaits
      121 llkradtics sheekrtenr vvsdlqkngy pssfirsswe prcrcel
//
LOCUS       XP_077501949             460 aa            linear   INV 25-NOV-2025
DEFINITION  ATP-binding cassette sub-family A member 17-like [Amblyomma
            americanum].
ACCESSION   XP_077501949
VERSION     XP_077501949.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645823.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..460
                     /product="ATP-binding cassette sub-family A member
                     17-like"
                     /calculated_mol_wt=51309
     Region          113..328
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            148..155
                     /site_type="other"
                     /note="Walker A/P-loop"
                     /db_xref="CDD:213179"
     Site            order(151..152,154..156,195,270..271,304)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:213179"
     Site            192..195
                     /site_type="other"
                     /note="Q-loop/lid"
                     /db_xref="CDD:213179"
     Site            246..255
                     /site_type="other"
                     /note="ABC transporter signature motif"
                     /db_xref="CDD:213179"
     Site            266..271
                     /site_type="other"
                     /note="Walker B"
                     /db_xref="CDD:213179"
     Site            274..277
                     /site_type="other"
                     /note="D-loop"
                     /db_xref="CDD:213179"
     Site            300..306
                     /site_type="other"
                     /note="H-loop/switch region"
                     /db_xref="CDD:213179"
     CDS             1..460
                     /gene="LOC144112970"
                     /coded_by="XM_077645823.1:1..1383"
                     /db_xref="GeneID:144112970"
ORIGIN      
        1 mmpvyiaaka enktarllyp lqpfhetgfy eavsmviqgt lypailslad aqafynlrwy
       61 larrfhggee sdeapeqqrg prlvavsmdp everegnlvn qvcrtkafkd vamvirnmqk
      121 avgfitpakv ldgvsillnr geclglvgin nsgkstllev lvglqvptgg gahtaalslg
      181 vdlralqqci gyapdgisea nmpaltvgel ldlmarlrgv lwrrqavlga lslvgrlked
      241 qminkcshge mkmllivaav mgvppvvlld epysdveply rneiirmvqv lkgaqamsii
      301 mtshrmshce vlcdrvavme agkiealgda lqmnqkygri yivtlrlppe rrfdyqfqri
      361 lvdmmqeefh qcsfgynykg mmsfrvgkty tswselftkm vafksnqelp efsvcditle
      421 hifvglarrq ilitgvrphn ylsgglsrlp srvetprrty
//
LOCUS       XP_077501966             502 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112985 [Amblyomma americanum].
ACCESSION   XP_077501966
VERSION     XP_077501966.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645840.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 44% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..502
                     /product="uncharacterized protein LOC144112985"
                     /calculated_mol_wt=55960
     Region          38..491
                     /region_name="Ebp2"
                     /note="Eukaryotic rRNA processing protein EBP2; pfam05890"
                     /db_xref="CDD:461770"
     CDS             1..502
                     /gene="LOC144112985"
                     /coded_by="XM_077645840.1:158..1666"
                     /db_xref="GeneID:144112985"
ORIGIN      
        1 msdsesldtd eelqeslakg elkpglyaia phvkkefinn taalkqklae meldldwvet
       61 ltmvnglapl tpelseqfgd melqknrkgt vvmgsnedpv hhdfkremaf yrqaqaavle
      121 giprlhqlgv vtrrpddyfa qmaksdthmt kvreklltkk valersekar klrelkkfgk
      181 kivegvavai tehgdiaqpi lfnrngepeh qpadeptfat ippfasgaah vvatlergee
      241 lddgliaqaa isrkesyvpe restvvtdaf tpsaacafdt eqpsdsdyvv lsnfwagctr
      301 appkedpeye iwlsnhdcqk ntyrksglme veaavtlfer slsrhglrys vmlcdgdsrp
      361 fnaiqeasvy gyipvrkedc vnhvkkrmgt alcnilnkhk grgqgsldfl entgkqkgpk
      421 paaagnqpgk gkrlsksqlk rqykdkkygy ggqrkrskfn tasssadveg fsrkkhnmap
      481 gagkkrpgka rrqnmknkqr rk
//
LOCUS       XP_077501986             167 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113000 [Amblyomma americanum].
ACCESSION   XP_077501986
VERSION     XP_077501986.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645860.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..167
                     /product="uncharacterized protein LOC144113000"
                     /calculated_mol_wt=18942
     Region          1..81
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            2
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238180"
     CDS             1..167
                     /gene="LOC144113000"
                     /coded_by="XM_077645860.1:1..504"
                     /db_xref="GeneID:144113000"
ORIGIN      
        1 mgasisvtma nltiediesr slatfnlkpk lflryvnncf cvvktseven llihlnstep
       61 aiqftvereq qgslpfldvl vtrhdnglrf svykkpthtg rylhfnsnhp tpqkasvits
      121 llkradtics sheekrtenr vvsdlqkngy pssfirsswe prcrcel
//
LOCUS       XP_077501998             720 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113008 [Amblyomma americanum].
ACCESSION   XP_077501998
VERSION     XP_077501998.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645872.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..720
                     /product="uncharacterized protein LOC144113008"
                     /calculated_mol_wt=77346
     Region          <42..215
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(76..81,116..117,149,211..212)
                     /site_type="active"
                     /db_xref="CDD:238827"
     Site            order(76..81,116)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238827"
     Site            117
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238827"
     Region          416..541
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     Site            order(419..422,451..453,456,480,519,537)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260008"
     CDS             1..720
                     /gene="LOC144113008"
                     /coded_by="XM_077645872.1:1..2163"
                     /db_xref="GeneID:144113008"
ORIGIN      
        1 mlrnldaheq arlldainsi wdsgilpesw staivvpirk tgsrrhccta asiadvvscq
       61 eeakqqgdva llilldvqaa fdglphstie qaldelgvrv ggtvssprpi ttgvpqgsvl
      121 spflfnlvma glpsaiqqgl chrvfcsvya qalqralvev qcflasrglv lsatksealf
      181 vhqrgarats rirtfridgt dlpwrqkvry lgltidhrlt ciladkphll hldhvgraae
      241 rllargnscs stwairlfea agtsavlyal plvtlrttrl rrletsrrkw icrllgvpqs
      301 sqvavtlaee gvlplnllll qrglthtdrl hhvpdgralr arlsgrphsr mgmlsaayme
      361 slgppashpa lpppptlppl qvsldfgsts krhtpaaaln qsaaafldei kgstllftds
      421 svlpatgqga aalvapqlna trtcrlpfpg ssfaaelagl hlaadliati phgpvvvlcd
      481 slpalqllpr pldnvattsl lsarlqtlea aghqislhwv pdhsgiegnd ladalaksah
      541 tdgspvsfav tqhdfarpli tqmvralhpd rriaaykpfr qlsdqlpgrd ralplrlrig
      601 ctwtpsswfc sqaaaclgla aaglamaerf vadepqptsr lsalradhvv ssdfesdltd
      661 ngsgdtifpd eclepvpplt shvrllsdqd selnllavsv aelssqstea daelhllwat
//
LOCUS       XP_077502011             621 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113013 [Amblyomma americanum].
ACCESSION   XP_077502011
VERSION     XP_077502011.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645885.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 33% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..621
                     /product="uncharacterized protein LOC144113013"
                     /calculated_mol_wt=69765
     Region          434..516
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     CDS             1..621
                     /gene="LOC144113013"
                     /coded_by="XM_077645885.1:1..1866"
                     /db_xref="GeneID:144113013"
ORIGIN      
        1 mcerdhkqvd pvqkvrsgpr eeqqaykcqs ctgcvkrhyh fvlrddavpv vqparrvpls
       61 lreplreelq rmeqggiiak vteptdwrkg snvppeqgnr rfsqdlrvas qtaefmwrqv
      121 lprlprrkap ptspqqdvvv lgnasvplkv nnllvlgpqy cehprldktq llslvrtaar
      181 rvrtdeagrc ikegvdslpq evkkgstrrl taavsalkda gvklvqsdke ggfvvvvndl
      241 ykekaqaava anfrqvktvk pskvqeqavq lceraglsrl assvkaskas slfvffsakt
      301 hkvsfpfrvi vsergtwqre vavflqkcls hlevedpfli rkpetvsefl qnecpahvga
      361 fsvdvkdlyy slpqsvviae iskcidrhgg tkfqnssgin vsmflemlrf ylkstfarvd
      421 dsyfiqkqgv ciesslapvl sdlflascdr qlqakldgti vkvmryvddf lvfyrksnaq
      481 qtvaqifdvf stelnnlqlt telptsnklr fldlelvlst tpleaaappa ailqgrklrt
      541 tlpaiqdspg lhvvkhrqtm nnreplppli qgdvvrvkkl fcdkkasvtq hcaqpksynv
      601 ftedgrllrr nrkhlmptke a
//
LOCUS       XP_077502047             587 aa            linear   INV 25-NOV-2025
DEFINITION  neuroglian-like [Amblyomma americanum].
ACCESSION   XP_077502047
VERSION     XP_077502047.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645921.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..587
                     /product="neuroglian-like"
                     /calculated_mol_wt=65665
     Region          137..208
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          245..249
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409416"
     Region          <247..306
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          259..263
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409416"
     Region          281..285
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409416"
     Region          295..300
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409416"
     Region          321..405
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     CDS             1..587
                     /gene="LOC144113034"
                     /coded_by="XM_077645921.1:1..1764"
                     /db_xref="GeneID:144113034"
ORIGIN      
        1 mfcaglanei tviknqtgkl ycnvtlstgg gsgdenpsqi rwykgaeliy mltsegrnvp
       61 irqmthrpsa swrdrlqvra trlpiwlsid pvglhdqghy qclvyygtdv krnvshflrv
      121 ieppenvvii fgtrpneirl spnqkvrqne gedlylechv iggkpeprir wlesgrkgsv
      181 sygsdrllkv prvprrfdnt sytceasnnn iteplrtsvt vllnlkpvnv sirnkdtplk
      241 agvlarvece sygsrpapqf swwldgkelv nnsrsvendl svlyfeptvq nngqkltcis
      301 rnplirdsdc vdiwtldvhf kpqitllfkg vrvqfvnitr gatvslecrt sgnprtgnit
      361 wylnkqrllr ktspkkgvff rdlsvvdivn anrnhsgsys csgsnavgst tsnevylrvl
      421 yepecmqgsd sylsaavdem vrvpcrvaad ppqvsyswsf rssldematr nltaprngsg
      481 gllfyqpkva adfgtvycwa qndigpmakp cifhvvlkes edamtsyvll aviitflfvl
      541 ilvpvvvviv vqtrerdvrk kgqnasvpav tpkdhtlpks mygdhsv
//
LOCUS       XP_077502059             161 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113042 [Amblyomma americanum].
ACCESSION   XP_077502059
VERSION     XP_077502059.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645933.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 21% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..161
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..161
                     /product="uncharacterized protein LOC144113042"
                     /calculated_mol_wt=18202
     CDS             1..161
                     /gene="LOC144113042"
                     /coded_by="XM_077645933.1:1..486"
                     /db_xref="GeneID:144113042"
ORIGIN      
        1 mreqtcerqh mgttnsqptr lqrclalplt sgsrphsrph rnawgapate pttpfkntin
       61 rdgkpssral ywlfrverlp tvqpyislvi aralktdgsl kqrtrkawss vsprnlaqhq
      121 rftvgyrrrf lplgphlavv tvtasimepy lplcrstcld d
//
LOCUS       XP_077502093             151 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113066 [Amblyomma americanum].
ACCESSION   XP_077502093
VERSION     XP_077502093.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645967.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 21% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..151
                     /product="uncharacterized protein LOC144113066"
                     /calculated_mol_wt=16759
     Region          <57..>122
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          59..88
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          90..121
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..151
                     /gene="LOC144113066"
                     /coded_by="XM_077645967.1:2..457"
                     /db_xref="GeneID:144113066"
ORIGIN      
        1 mvrkkqldrt kkrsrwktdd eeqkwdlalc halealqaiv fqsandrska allkelykra
       61 pllaaaycgh kpsveallka gsdlnvtard ggtpvmlais qghtdialql leanadativ
      121 nqasiaytrq pgreeqeqge kayfffecrv e
//
LOCUS       XP_077502135             200 aa            linear   INV 25-NOV-2025
DEFINITION  mesoderm induction early response protein 2-like [Amblyomma
            americanum].
ACCESSION   XP_077502135
VERSION     XP_077502135.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646009.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..200
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..200
                     /product="mesoderm induction early response protein
                     2-like"
                     /calculated_mol_wt=22771
     Region          47..94
                     /region_name="ELM2"
                     /note="ELM2 domain; pfam01448"
                     /db_xref="CDD:460214"
     Region          137..181
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cl21498"
                     /db_xref="CDD:473887"
     Site            order(137,167..168,170..171,173..175,177..179)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:212559"
     CDS             1..200
                     /gene="LOC144113087"
                     /coded_by="XM_077646009.1:1..603"
                     /db_xref="GeneID:144113087"
ORIGIN      
        1 msgskdtscs assastarpp sepghsrtyq lrskgpvelp skaarpievg pqyqadiper
       61 lsehetppye tedellwdps klsdsdvkhy lersnaggst rgeeealsll qhcehsirea
      121 lkrqrmgtat ttktrpwsee erqsfengfr lygkdfckiq vrelparsvg elveyyylwk
      181 kterhdtfvr lpkkrskkde
//
LOCUS       XP_077502142             118 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113094 [Amblyomma americanum].
ACCESSION   XP_077502142
VERSION     XP_077502142.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646016.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 26% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..118
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..118
                     /product="uncharacterized protein LOC144113094"
                     /calculated_mol_wt=12894
     CDS             1..118
                     /gene="LOC144113094"
                     /coded_by="XM_077646016.1:1..357"
                     /db_xref="GeneID:144113094"
ORIGIN      
        1 mcaacnrprr aaarvpassm qpssshgrgp swaallllll acvheseqgk wlklagaaal
       61 lgggrgiipi pipihygrdd hhhhhhhphh yhhhhhptyh hhhghghygg hggyggww
//
LOCUS       XP_077502173             612 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113131 [Amblyomma americanum].
ACCESSION   XP_077502173
VERSION     XP_077502173.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646047.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..612
                     /product="uncharacterized protein LOC144113131"
                     /calculated_mol_wt=64489
     Region          409..>454
                     /region_name="ZapB"
                     /note="Cell division protein ZapB; pfam06005"
                     /db_xref="CDD:428718"
     Region          <428..>560
                     /region_name="COG4372"
                     /note="Uncharacterized protein, contains DUF3084 domain
                     [Function unknown]"
                     /db_xref="CDD:443500"
     CDS             1..612
                     /gene="LOC144113131"
                     /coded_by="XM_077646047.1:1..1839"
                     /db_xref="GeneID:144113131"
ORIGIN      
        1 mapvgdpsal aapnqvtgyn pqslqivtmd thqsedttqg edplpteddy lqdmvrvwrg
       61 knaaaasrda gagatqqqap assahtqggs alnaaskssk qlrwrpqntp riekddivvv
      121 lkprntvglk atfgpgraga adrsilgdda naglevwpvw dqnvlvcalk svdaaekllr
      181 divlpvgere lpfrghpkps gafcrgvvtv spdetsesvk rklsaeqnvv svrklggtpv
      241 avitfagtkv prtvlysyer lpvrlykktv pachrcgtvg hradacprpq pgrckrcgvq
      301 vldatpdgpa ehdcvpscli cggghptgap gcagwfrkai kpsspsgskp ktktgpkqap
      361 ppkakkapad qetkakpgts kpvksatqav ppvlnardfp plapvqpqes swsravsgpp
      421 trpsptetal qqqveelrrq nqilarkiqe leakqagsse pmqeaepdde ddgssvtscl
      481 tsvsrqcgdt vvgsagrvag lealqrqteq leeqvaalpn qimsavress qdmfkaaltq
      541 alpdlmaqvt dsvlkaaqpw vstqiknats cesphlkrkt asrqaaeehg sgsvaggsvr
      601 piagvalapg qs
//
LOCUS       XP_077502252             308 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113184 [Amblyomma americanum].
ACCESSION   XP_077502252
VERSION     XP_077502252.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646126.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..308
                     /product="uncharacterized protein LOC144113184"
                     /calculated_mol_wt=33341
     CDS             1..308
                     /gene="LOC144113184"
                     /coded_by="XM_077646126.1:261..1187"
                     /db_xref="GeneID:144113184"
ORIGIN      
        1 mkptetpkla rrvglssest kptteirrpr dpphsskdva atpsstnshh hhqlahmkra
       61 ssvgaasrqs vapkavhfqa ppavltskls stksstndpp vvgkslirrs ksfshndpas
      121 tnasflgeyq glvkkaqhdp salsthqlme lvsvvchtai edvqnaepaa qlclffiake
      181 rgetfleslm cacrewfser dqllrqldas tavpcrwtay vcflaqllvg lqglsgaags
      241 ddhgaattlt anrtlclvtl lydcchiilr ppslgnaaea sspllffwsr qcirahvvsw
      301 dsfnvpsh
//
LOCUS       XP_077502266              98 aa            linear   INV 25-NOV-2025
DEFINITION  MIF4G domain-containing protein-like [Amblyomma americanum].
ACCESSION   XP_077502266
VERSION     XP_077502266.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646140.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..98
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..98
                     /product="MIF4G domain-containing protein-like"
                     /calculated_mol_wt=10838
     Region          <11..65
                     /region_name="MIF4G"
                     /note="MIF4G domain; cl02652"
                     /db_xref="CDD:413423"
     CDS             1..98
                     /gene="LOC144113187"
                     /coded_by="XM_077646140.1:1..297"
                     /db_xref="GeneID:144113187"
ORIGIN      
        1 mttargasqm eclrsvltia gegiercapq rmetlvqrlr eafllpdlsa qarktllevi
       61 elrasgwqfn laqelyhfpy tqasstaces ptppkcrp
//
LOCUS       XP_077502286             171 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113198 [Amblyomma americanum].
ACCESSION   XP_077502286
VERSION     XP_077502286.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646160.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 37% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..171
                     /product="uncharacterized protein LOC144113198"
                     /calculated_mol_wt=17815
     Region          88..151
                     /region_name="SVWC"
                     /note="Single domain von Willebrand factor type C;
                     pfam15430"
                     /db_xref="CDD:464713"
     CDS             1..171
                     /gene="LOC144113198"
                     /coded_by="XM_077646160.1:1..516"
                     /db_xref="GeneID:144113198"
ORIGIN      
        1 merlqivkal araanvqyrg rvlgcesseg eradalqdma nsvgfrhglv htvakskhal
       61 taalacvlwl vgdasvgmvg vqikngrcvl ngteiqngks vsletpcesh vcdataksvq
      121 imgcgpveap pnctpvngtg ifpkccrhal ckssstqplk vtalvsnltk c
//
LOCUS       XP_077502411             154 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113293 [Amblyomma americanum].
ACCESSION   XP_077502411
VERSION     XP_077502411.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646285.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..154
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..154
                     /product="uncharacterized protein LOC144113293"
                     /calculated_mol_wt=17388
     CDS             1..154
                     /gene="LOC144113293"
                     /coded_by="XM_077646285.1:1..465"
                     /db_xref="GeneID:144113293"
ORIGIN      
        1 mhaksevpal nsvlkavned ddrpnfkkkt lcklmkdvif tsekgkwnla liersdiiaw
       61 rrrciiylde twvkaghtke yvwqdktvks sqdaflkglt telaapsgkg arlilvhvvg
      121 satgfvrdaa dffrakkgnh adyhsemncl yfkk
//
LOCUS       XP_077502436             334 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113317 [Amblyomma americanum].
ACCESSION   XP_077502436
VERSION     XP_077502436.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646310.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..334
                     /product="uncharacterized protein LOC144113317"
                     /calculated_mol_wt=38002
     CDS             1..334
                     /gene="LOC144113317"
                     /coded_by="XM_077646310.1:1..1005"
                     /db_xref="GeneID:144113317"
ORIGIN      
        1 masstasvat spvrvfskvy tsklsrhakq vianvysrtr trfpeksvre alsaasedtg
       61 vsprtvakik aerlcgplay pkkrprdvki sslrtakkdn ftvhvirlkg hsmyakrdvp
      121 tldsvlkavs edddlpnlkk ttlwmlmkdv gftfekrkrn laliersdii awrriylrai
      181 kefrsqgrci iylhetwida ghikeyvwrd ktvkssqdaf lkglttelaa psgkgarlil
      241 vhvggsatgf vrdaagffra kkrkhadyys eiddyfescf tdsflqtfrp islsswsmlh
      301 iiarllknhl pslraklifs fgsqrkvfrg hrtw
//
LOCUS       XP_077502450             788 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113322 [Amblyomma americanum].
ACCESSION   XP_077502450
VERSION     XP_077502450.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646324.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..788
                     /product="uncharacterized protein LOC144113322"
                     /calculated_mol_wt=86876
     Region          <653..>770
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..788
                     /gene="LOC144113322"
                     /coded_by="XM_077646324.1:223..2589"
                     /db_xref="GeneID:144113322"
ORIGIN      
        1 msemrsnglg sssrsgedes skspeplrvr hvtlernhae rlqeqescva aaglteggdi
       61 qpafgdspsl ldvmnmfknv idtevrkdse gassarnlee snaalrrlvn tllehievlv
      121 tcldevkrea enrtdqmqrr lrssadvtrs tvytlsewge evqslvsrkc raeeaahdmk
      181 hmvdalqken nqlrqhsanl shdiqsllti isearatgnw vmdcvtfcei spedvygpvf
      241 nlsaqqadvv vphavideds evhlenpgle vqeagisevc pqsnilfqrp vianedtlsl
      301 glsssgsdsp prfstpyshr ngqqvthkvn apveddpeni rcvsqlhare tqsseksesl
      361 pllplaaqec pelynesaye qrgeslesml iswkgrlnnv rrrlifnspr gepaapehkf
      421 irsesvgmtn svnslvatds encthvtvgi qtgatvtasn aevhyhprqf qdvvtqtraa
      481 grsfdvvdtq tdaeyeerdd lstsfpasis mlelenilel gsgisfltrs aseessglfs
      541 swrksgrsvq kkrllfntrr esgvpdhhrr tqqvnsmvnt eralvgidae gstpvsvsiq
      601 tdativacce sahchppelk dvitqteayd ktfgvtgtqt dvewenfpst ghqegdrelr
      661 slkerlnlaa eeaqsknmla tqlqthldss lkqvemsaqa lqnvekklql sreecetlkk
      721 qlistqgkle laekaaever manaqskaei qnlaltvqhl qaalvnlkkn ggppprgsss
      781 gmpkvhdv
//
LOCUS       XP_077502462             107 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-scruin-like [Amblyomma americanum].
ACCESSION   XP_077502462
VERSION     XP_077502462.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646336.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 26% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..107
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..107
                     /product="alpha-scruin-like"
                     /calculated_mol_wt=11853
     Region          <57..>107
                     /region_name="NanM"
                     /note="N-acetylneuraminic acid mutarotase [Cell
                     wall/membrane/envelope biogenesis]; COG3055"
                     /db_xref="CDD:442289"
     CDS             1..107
                     /gene="LOC144113329"
                     /coded_by="XM_077646336.1:1..324"
                     /db_xref="GeneID:144113329"
ORIGIN      
        1 msspttgeta aqpieyrtpe hgaksalglr vepewqsagl relqwtrqpc dlhfpvillv
       61 ggldprnplg sgsavlkyhp fkdrwmlvga mpeprnyhaa vyvnrvy
//
LOCUS       XP_077502478            1399 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113335 [Amblyomma americanum].
ACCESSION   XP_077502478
VERSION     XP_077502478.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646352.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 38% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1399
                     /product="uncharacterized protein LOC144113335"
                     /calculated_mol_wt=140830
     Region          <140..465
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          737..>920
                     /region_name="NanM"
                     /note="N-acetylneuraminic acid mutarotase [Cell
                     wall/membrane/envelope biogenesis]; COG3055"
                     /db_xref="CDD:442289"
     Region          747..792
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          795..842
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          846..895
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          897..943
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     CDS             1..1399
                     /gene="LOC144113335"
                     /coded_by="XM_077646352.1:1..4200"
                     /db_xref="GeneID:144113335"
ORIGIN      
        1 mpserspwkd fgmrgfwsvk ygvgaqaigv vapvigvvvp giekvptppa lpepllayta
       61 llaaparpap paplalpqal aflappaqsa lpalqaaltl paaptrpeal ppppaasals
      121 aalappatss ppapparrpg kpgprdapds pgspdppnap gppsglgppd rvsspsgpgp
      181 lgapalpaas avpvpsplle isvlpapppt sgppsgpepl dmpgpsgalg slggtdapgt
      241 ssppggvgnp gvlgplsths ppgspyspsd fgspgdpspp ggignpgisg ppstpgpssg
      301 paspgapgnq dglalpsgpg ppgapgphdg lalpsgleps gharpsgrsw psymprsphy
      361 srpsrnpgfs swpvlsrpqp sqrpwpsrrp qpsrlprpsq rhwpsrcqrp srcprpsrha
      421 rpsrcphpyr hnlfslslpv lpvtpglpgg pdtpvglcpp gdptphgafg pssgrgppga
      481 tgppgapglp gapsprdtpg lpgalgpprg lgplatlgps ggpnspggph slgglgnpgd
      541 pgppgmpgpp gtpvtpsglg prftpgppgg pdspsgrgfp stprlpadli sfgaprphdg
      601 pgppgtpalp arlaltaasa lpallallaa salpapltll aasahpaplt lfaasalpap
      661 lalpappgda vlsydpgsns wstygfmpqp mcyhtavvvg kndvvvaggl dpqcltssgs
      721 mqpsgkaflf cntrkrwmel ptmhrgrafh aavgcydrmi vfggidrtgr tlnsaemydl
      781 kakqwsfihp mpmalmgmaa atidrrvyiv ggithgldgd tlqnsvyafd lskdtwtelp
      841 amtekrafcs avpvqpdlwl vggvvelkpe vkctsridvl rlasstlerr cdmpsprhsv
      901 clakigyevy lvggqddnss pcddvvlfnp lrrsvipcep lpsdlvgaat avmpgdcfdr
      961 plcrwiwptt kttphnrdra vtviqkarlh retcipapkg dsdhnasgff gaaqyrpssd
     1021 paappswrqr kplhcrhpap palpqavqtl qaasalpapp ahpaslaipa ppahparpal
     1081 hraltlpapp avttaspfkv apalpaaaal papadllapp vlrvpsralq trqairalla
     1141 llhasvpsll salqearilq lartlpasal aeplafpapp glttasalpa alalpvpkai
     1201 pvpqalparq alpvpppvpa lpvllatsah ssrpalksal tlpedcalpa sppltapsal
     1261 pvaaalpapp vllappvfpv ppsprdtpgh pgalgpprgl gplatlgpsg gpdypggpns
     1321 fgglgnpgep gppgtpgppg tpvppsglgp rftpgppggp dsprgrgfrs tpglpadlvs
     1381 fgapgphdgl gppgtpgtp
//
LOCUS       XP_077502490             577 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113340 [Amblyomma americanum].
ACCESSION   XP_077502490
VERSION     XP_077502490.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646364.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..577
                     /product="uncharacterized protein LOC144113340"
                     /calculated_mol_wt=58132
     Region          <238..507
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..577
                     /gene="LOC144113340"
                     /coded_by="XM_077646364.1:1..1734"
                     /db_xref="GeneID:144113340"
ORIGIN      
        1 melqegrilh vawtlpaasa lpaplafsap palttasafs aapalpvppv llvppalpap
       61 qalpvppavp arpalqafla flaasaqssh palqaartvp vesalpapla clatssvsap
      121 pavttvpall lppallapav lpgpdlarvd lpaapnlrtc lrlwaplalp vartlpappa
      181 hpaasaipap parpaalaip appalpaass plaalalpap pglpapldlp vasgaltpps
      241 psanpaaspl mapsdlpvpp alraasalsa tpahpaasaf paplvllaas tllaaavlla
      301 tlalpaspal tavpalsapr lsrrarpsvp aalseprhpp svsalsppaa hpstpalsaa
      361 pvlsesppks pvcrrrppgv lgypgapspp sglgypgtsg pparqalqav mtlpappalp
      421 ttspfpaata llapstlpll alqkvsvpsl rtelqearih qvsqtlqaas alsaphafsa
      481 pptlttassy qaapalqapp vllatlvalf prpfeharps gcswpfwrpr ytehaqpsrw
      541 pglsqrprls rrpqpirrpr lsrhlrsaqh grpsrrp
//
LOCUS       XP_077502501             533 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113347 [Amblyomma americanum].
ACCESSION   XP_077502501
VERSION     XP_077502501.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646375.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 32% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..533
                     /product="uncharacterized protein LOC144113347"
                     /calculated_mol_wt=54183
     Region          <1..115
                     /region_name="PHA03098"
                     /note="kelch-like protein; Provisional"
                     /db_xref="CDD:222983"
     Region          5..52
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          53..84
                     /region_name="Kelch_5"
                     /note="Kelch motif; pfam13854"
                     /db_xref="CDD:433528"
     Region          56..101
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     CDS             1..533
                     /gene="LOC144113347"
                     /coded_by="XM_077646375.1:17..1618"
                     /db_xref="GeneID:144113347"
ORIGIN      
        1 mtekrafcsa vpvqpdlwlv ggvvelkpev kctsridvlr lasstlerrc dmpsprhsvc
       61 lakigyevyl vggqddnssp cddvvlfnpl rrsvmprepl psglagaatt vmpgdcfdrp
      121 lcrwiwarlh retcipaskg ysdhnasgff gaaqyrpssd paappswrqr kplhcrhpap
      181 palpqavesl qaasalpapp ahpaslaipa ppahparpal hpaltlpapp avttaspfpv
      241 apalpaaaaq papadllapp vlrvpsralq trqairtlla llhasvpsll salqearilq
      301 lartlpasal aetlafpapp alttasalpa elalpvpkai pvpqalparq alpvpppvpa
      361 lpvllatsah ssrpalksal tlpedcalpa spplmapsal pappvllapp vfpvppsprd
      421 tpghpgalgp prglgplatl gpsggpdypg gpnslgglgn pgepgppgtp gppgtpvpps
      481 glgprftpgp pggpdsprgr gfrstpglpa dlvsfgapgp hdglgpsgtp gtp
//
LOCUS       XP_077502514             221 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113349 [Amblyomma americanum].
ACCESSION   XP_077502514
VERSION     XP_077502514.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646388.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 21% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..221
                     /product="uncharacterized protein LOC144113349"
                     /calculated_mol_wt=24266
     Region          127..169
                     /region_name="Kelch_3"
                     /note="Galactose oxidase, central domain; pfam13415"
                     /db_xref="CDD:433188"
     CDS             1..221
                     /gene="LOC144113349"
                     /coded_by="XM_077646388.1:712..1377"
                     /db_xref="GeneID:144113349"
ORIGIN      
        1 mgprlsqnrg khievgryec rapeagtiet vdhrmaeekr rhtevdrdkc capgsylmkt
       61 mnrrarefra chssqncvaa skesephycs fltkiltqvk pktgdkrdyi ttdvltrkvl
      121 trkimsfggi tpprpndcpk gdavlsydpg snswstygfm pqpmcyhtav vvgkndvvva
      181 asaaaayncs aytsdpvtrh rqcaclsatv psaqaawtps a
//
LOCUS       XP_077502527            1067 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113353 [Amblyomma americanum].
ACCESSION   XP_077502527
VERSION     XP_077502527.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646401.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 21% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1067
                     /product="uncharacterized protein LOC144113353"
                     /calculated_mol_wt=114072
     Region          307..349
                     /region_name="Kelch_3"
                     /note="Galactose oxidase, central domain; pfam13415"
                     /db_xref="CDD:433188"
     Region          390..637
                     /region_name="NanM"
                     /note="N-acetylneuraminic acid mutarotase [Cell
                     wall/membrane/envelope biogenesis]; COG3055"
                     /db_xref="CDD:442289"
     Region          400..445
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          448..495
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          499..548
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          550..595
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          <737..1067
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..1067
                     /gene="LOC144113353"
                     /coded_by="XM_077646401.1:1..3204"
                     /db_xref="GeneID:144113353"
ORIGIN      
        1 mkardeprdf yvpshdgedw gcgppeagfe ktmyhpssek narrtevvrg eceapggyiv
       61 esmnhgtrln rallaqvgrd ecsketidrr isqkrggisv agraqsrile fdlierierr
      121 irenkewcad vgrheyrdpg pdivetmerf esesgwwrrk rdreqseapa svfaegmgpr
      181 lsqyrgkhie vgrykctape agtietvdhr vaeerrrhte vdrdkccapg sylmktmnrr
      241 arefrachss pncvaaskes ephycsfltk iltqvkpktg dkrafyrdyi ttdvltrkvl
      301 trkimsfggi tpprpndcpk gdavlsydpg snswstygfm pqpmcyhtav vvgkndvvva
      361 ggldpqcvts sgsmqpsgka flfcntrkrw ielptmhhgr afhaaigcyd rmivfggidr
      421 tgrtlnsaem ydlnakqwsf ihpmpmalmg maaatidrrv yivggithgl dgdtlqnsvy
      481 afdlskdtwt elpamtekra fcsavpvqpd lwlvggvvel kpevkctsri dvlrlasstl
      541 errcdmpspr hsvclakigy evylvggqdd nsspcddvvl fnplrrsvmp ceplprglag
      601 aaaavmpgdc fdwplfrwiw pttkttphnr dravtvtqka rlhretcipa pkgysdrnas
      661 gffgaaqyrp ssdtaappsw rqrkplhsrh palpasmalt appalpqava tlqaasalla
      721 ppahpaslai pappahparp alhpaltlpa pptiktaspy paapalpapq avttaspfpv
      781 apalkaaaal papadllapp vlrvpsralq trqairalla llhasvpsll salqearvlq
      841 lartlpasal aarlafsapp vlttpsafpa alalpvpkai pvpqalpvpp pvpalpvlla
      901 tsalssrpal qaaltlpedc alpatpplta psalpvaaal pappvllapp vfpvplsprd
      961 tpghpdalgp prglgplatl gpsggpdypg gpnslgglgn pgepgppgtp vppsglgsrf
     1021 tpgppggtds psgrgfrstp glpadlvsfg apgphdgpgp qgtpgtp
//
LOCUS       XP_077502536             419 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113359 [Amblyomma americanum].
ACCESSION   XP_077502536
VERSION     XP_077502536.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646410.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 16% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..419
                     /product="uncharacterized protein LOC144113359"
                     /calculated_mol_wt=47082
     Region          <303..>418
                     /region_name="NanM"
                     /note="N-acetylneuraminic acid mutarotase [Cell
                     wall/membrane/envelope biogenesis]; COG3055"
                     /db_xref="CDD:442289"
     Region          340..393
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     CDS             1..419
                     /gene="LOC144113359"
                     /coded_by="XM_077646410.1:72..1331"
                     /db_xref="GeneID:144113359"
ORIGIN      
        1 mrardeprdf yvpshdgedw gcgppeagfe ktmyhpssek narrtevvrg eceapggyiv
       61 esmnhgtsln rallaqvgrd ecskgtidrr isqkrggisv agraqsrile fdliermerr
      121 isenkewcad vgrheyrdpg pdivetmerf isesgwwrrk rdreqseapa svfaeamgpr
      181 lsqnrgkhie vgryecrape agtietvdhr maeerrhhte vdhdkccapg sylmktmnrr
      241 arefrachss pncvaaskes ephyssfvtk iltqvkpktg dkrdyittdv ltrkvltrki
      301 msfggitppr pndcpkgdav lsydpgsnsw stygfmpqpm cyhtavvvgk ndvvvaggld
      361 pqcvtssgsm qpsgkaflfc ntrkrwielp tmhrgrafha avgcydrmiv fggidrtgr
//
LOCUS       XP_077502556             171 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113382 [Amblyomma americanum].
ACCESSION   XP_077502556
VERSION     XP_077502556.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646430.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..171
                     /product="uncharacterized protein LOC144113382"
                     /calculated_mol_wt=19117
     CDS             1..171
                     /gene="LOC144113382"
                     /coded_by="XM_077646430.1:22..537"
                     /db_xref="GeneID:144113382"
ORIGIN      
        1 mttivipasa nksdmaslkv ackylryyqe cvssqstlmc seegpsvvds ikqsaseyyn
       61 aynwtcdatk dlpscsketf qeqaktcfes yitkiviphs raslssnyts lkiacaavhe
      121 yqdcvstkll ttcpeeykrn drllemftdg vygmynwtca vrstqentki a
//
LOCUS       XP_077502570             175 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113398 [Amblyomma americanum].
ACCESSION   XP_077502570
VERSION     XP_077502570.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646444.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 20% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..175
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..175
                     /product="uncharacterized protein LOC144113398"
                     /calculated_mol_wt=18860
     CDS             1..175
                     /gene="LOC144113398"
                     /coded_by="XM_077646444.1:1..528"
                     /db_xref="GeneID:144113398"
ORIGIN      
        1 mvigsspsip rpkadtryss trlawsthgw rtlfhnvrgg aptgppvpvg vlrrwqaach
       61 rhrpgprgga ahaedfrgqa pntpaaanrr ntssspngqc delgngcsrh psaasevlam
      121 amhepypgrq wrsvtfddak rrviatdqda evdtlnmkct vgtlptlrps rtdcc
//
LOCUS       XP_077502585             763 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113403 [Amblyomma americanum].
ACCESSION   XP_077502585
VERSION     XP_077502585.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646459.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..763
                     /product="uncharacterized protein LOC144113403"
                     /calculated_mol_wt=86034
     Region          7..101
                     /region_name="EEP"
                     /note="Exonuclease-Endonuclease-Phosphatase (EEP) domain
                     superfamily; cl00490"
                     /db_xref="CDD:469791"
     Region          370..640
                     /region_name="RT_nLTR_like"
                     /note="RT_nLTR: Non-LTR (long terminal repeat)
                     retrotransposon and non-LTR retrovirus reverse
                     transcriptase (RT). This subfamily contains both non-LTR
                     retrotransposons and non-LTR retrovirus RTs. RTs catalyze
                     the conversion of single-stranded RNA into...; cd01650"
                     /db_xref="CDD:238827"
     Site            order(458..463,518..519,549,551..552,636..637)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238827"
     Site            order(458..463,518,551)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238827"
     Site            519
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238827"
     CDS             1..763
                     /gene="LOC144113403"
                     /coded_by="XM_077646459.1:1..2292"
                     /db_xref="GeneID:144113403"
ORIGIN      
        1 mqkfklllqk asqlagdhpl ivagdfnapy hvwgykhdiv kgrdlwqnaa efdltlvtdp
       61 afptrigtst crdttpdlcf vknaskpswc nlaegldsdh yinqvvfeve grlpraltfi
      121 dwdkfrmire ergrsgkefe gpyrpsledw vtqvredvng atkeistdle vdcgdsrlah
      181 lieakrsvla rwkgqrlnrr lrkkiselnk lieehcklls rqqwnevcns vdeqarngrs
      241 wgllkhllnd sntrvsqghv laravheavg saseeelver lskkylpvad pdalltehay
      301 tgednfslde afpveevrtv lhnlnsksap gpdgisnkll knlddrsiky lteevntmwr
      361 dgvvpeqwkt altvlipkag kapsidnlrp isltscvrkv aehaalnrlt ryleenevyp
      421 htmigfragl stqdamklik hqvidgdrrg vkailgldle kafdsvrhsy ilksisdlgl
      481 garfhdyfrs flscrkatlr vaelesktln lvdrgtpqga visptlfnla tvglakklle
      541 ieaikhtmya ddaaiwcpgn segyiecvlq eavdvvetyl dptglrcsps kselllyspt
      601 rrgpkprgwi pvdklsiqlr trngdliprv dkirvlgmli esngantlti qkiiaktesa
      661 vrlvkrvanr qtglkedsli rlmhafvlch fthvaamhkw kpsekekldt hirkttkkvl
      721 gvpmctsndr llqleihtld eiaeaqertq mvrlsrlapv grf
//
LOCUS       XP_077502605             414 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113412 [Amblyomma americanum].
ACCESSION   XP_077502605
VERSION     XP_077502605.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646479.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..414
                     /product="uncharacterized protein LOC144113412"
                     /calculated_mol_wt=46924
     Region          22..43
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          54..73
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          312..406
                     /region_name="MULE"
                     /note="MULE transposase domain; pfam10551"
                     /db_xref="CDD:463145"
     CDS             1..414
                     /gene="LOC144113412"
                     /coded_by="XM_077646479.1:61..1305"
                     /db_xref="GeneID:144113412"
ORIGIN      
        1 masvfpnqse rqpehgdhdr rcgvcdkvft qrknmlqhmr mvhkletkga flhcdqcssl
       61 lstlenlvhh ynvahkfpae nlqlsfgndk efhdwkgqee asqvckfvis tgekrltngc
      121 trtyyacnrs gvarpgrgnk tramksqgsc rsgrtclsfl ivtrdegtvk vsyqrthygh
      181 dadvehlsmt dqerasiadd lskgvpmrti lkkiresvts kltplhltnr mdlhnikrqy
      241 nltvperchd ndavsvsiwv qsmrnkgqpv vrlykeqevv dptntfstkd falalmtepq
      301 eellkvlgtg tvcvdsthgt tgyqfelvtv lvldeygagl pmayficsti nektlsaffe
      361 slksstgals artfmtddas eyfkawstvm gppqhrllct whvdknwkrr isrr
//
LOCUS       XP_077502617             300 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113418 [Amblyomma americanum].
ACCESSION   XP_077502617
VERSION     XP_077502617.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646491.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..300
                     /product="uncharacterized protein LOC144113418"
                     /calculated_mol_wt=33312
     Region          38..83
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          118..285
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..300
                     /gene="LOC144113418"
                     /coded_by="XM_077646491.1:1..903"
                     /db_xref="GeneID:144113418"
ORIGIN      
        1 mqsaktvkfy tgfvstamft aflqfvlsaw kpscatwlda etqlimvlir lhlglltqdl
       61 scrfgisngt vssifhawfd vlaanmtkli iwpsrqtlqa nrpvafqdpf fdgvtgiidc
      121 tevfiqrpil mtarsqtysh ykhhntvkil viispsgait fvskawggrt pdkeitlrsg
      181 lldkvkegdv flvdrgfrcg emfagrgatl lmlsltknra qlpgvevtts rkvssvrihv
      241 ekaiqrltvf rvfqtvlpln fvkrtgdknl atidkvvvvc salvnlqtpi iknsqrrsas
//
LOCUS       XP_077502636             371 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113435 [Amblyomma americanum].
ACCESSION   XP_077502636
VERSION     XP_077502636.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646510.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 26% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..371
                     /product="uncharacterized protein LOC144113435"
                     /calculated_mol_wt=39660
     CDS             1..371
                     /gene="LOC144113435"
                     /coded_by="XM_077646510.1:178..1293"
                     /db_xref="GeneID:144113435"
ORIGIN      
        1 mvtvdaqsri pevadpqhqe tasekpssst stadlenapr esflpnkrke eadgqefvgl
       61 kpadtlvang aeaygvdqrs prqinrhppr alchmsspsp gesedttafg faevigrnir
      121 rgkktgitpr dfdlsslgsp sliaavpkfp elpsphkvtt asaedsnske lrtshqkfpe
      181 vlptlpssah sekvprlrip pnkekeptng pdldslvpay spvanggees cgidqrspqq
      241 isrhasrssp ehaedstgly flrligrnit crrrtgitsc evdvdsekgs hasfarsken
      301 epaavtglgg mgpsthpaat iepvpgvdqg nsrptkrqga ktsrrrgasr etsedgttsc
      361 eyapsyglls v
//
LOCUS       XP_077502650             157 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113442 [Amblyomma americanum].
ACCESSION   XP_077502650
VERSION     XP_077502650.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646524.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..157
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..157
                     /product="uncharacterized protein LOC144113442"
                     /calculated_mol_wt=17662
     CDS             1..157
                     /gene="LOC144113442"
                     /coded_by="XM_077646524.1:87..560"
                     /db_xref="GeneID:144113442"
ORIGIN      
        1 maspgasslv kfnirwgnrs nqssimntse yidtlkkrlr avimknkila ralaaskkei
       61 selkksngrq qkvfnlglpq vktwlkmarq hshtmyqyqs raltlldeim npgvdftlar
      121 vsdadddadi qdrcfpsgks tflayvrgvr cvfksfv
//
LOCUS       XP_077502661             224 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113448 [Amblyomma americanum].
ACCESSION   XP_077502661
VERSION     XP_077502661.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646535.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 48% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..224
                     /product="uncharacterized protein LOC144113448"
                     /calculated_mol_wt=24683
     Region          <1..60
                     /region_name="GT1"
                     /note="myb-like, SANT family; cl23759"
                     /db_xref="CDD:474047"
     CDS             1..224
                     /gene="LOC144113448"
                     /coded_by="XM_077646535.1:1..675"
                     /db_xref="GeneID:144113448"
ORIGIN      
        1 mrtkfyvies ksdgaslerk ktwkllteqy ncrtdvrprt eaqlrkcwdn lkekwrraka
       61 advkaifgtg ggppppsqld eelhrvgdva shmgtrvynp fdtdgshysn sgplqctptv
      121 galleatqse pqaeadefdf pwcratknsq aesdeaaeva evapddqeay snqdlqeqqc
      181 sdgdhnapil attcevilpa aqpagyqrrk aapapkesvc lrqs
//
LOCUS       XP_077502668             203 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113455 [Amblyomma americanum].
ACCESSION   XP_077502668
VERSION     XP_077502668.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646542.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 14% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..203
                     /product="uncharacterized protein LOC144113455"
                     /calculated_mol_wt=22771
     CDS             1..203
                     /gene="LOC144113455"
                     /coded_by="XM_077646542.1:85..696"
                     /db_xref="GeneID:144113455"
ORIGIN      
        1 maspgasslv kfnirwgnrs nqssimntse yidtlkkrlr tvimknkila qalaaskrei
       61 selkksngrq qkvfnlglpq vktwlkmarq hsqtmyqyqs raltlldeim npgvdftlar
      121 vsdadddadi qdrrpslhpp gkmskaqrge lsvipeeded vnasrrmllg tftldsetwl
      181 raadtssrrl rcqvpstpas fma
//
LOCUS       XP_077502679             551 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113464 [Amblyomma americanum].
ACCESSION   XP_077502679
VERSION     XP_077502679.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646553.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..551
                     /product="uncharacterized protein LOC144113464"
                     /calculated_mol_wt=59649
     CDS             1..551
                     /gene="LOC144113464"
                     /coded_by="XM_077646553.1:1..1656"
                     /db_xref="GeneID:144113464"
ORIGIN      
        1 mlvkfnirwg nrsnqssimn tseyidtlkk rlravimknk ilaralaask keiselkksn
       61 grqqkvfnlg lpqvktwlkm arqhshtmyq yqsraltlld eimnpgvdft larvsdaddd
      121 adiqdrrpsl hppgkmskaq rgelsvipee dedvnasrrm llgtftldse trlraaetie
      181 rksrgsmsss erpsgssgkp raptpkgsvq slgckvrgpw wtdggagdld yaflespnpt
      241 aplrqgnepg ekmvtvyaqs ripevadpqh qetasekpss ststadlena presflpnkr
      301 keeadgqefv glkpadtlva ngaeaygvdq rsprqinrhp pralcrmssp spgesedtta
      361 fdflrligrn itcrrrtgit scdldlssle spspaaavpe isdlasaqmv tratgqekss
      421 hevgtasqka pdepptlasr tdsekgshas farskenepa avtglggmgp sthpaatiep
      481 vpgvdqgnsr ptkrqgakts rrrgarrets edgttssvas stvdgesvar stsgrplrsa
      541 aigknyresd i
//
LOCUS       XP_077502688             316 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: L-fucose kinase-like, partial [Amblyomma
            americanum].
ACCESSION   XP_077502688
VERSION     XP_077502688.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646562.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 2 indels
            ##RefSeq-Attributes-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         <1..316
                     /product="LOW QUALITY PROTEIN: L-fucose kinase-like"
     Region          <16..301
                     /region_name="fkp"
                     /note="bifunctional
                     fucokinase/L-fucose-1-P-guanylyltransferase; Provisional;
                     PRK13412"
                     /db_xref="CDD:237379"
     CDS             1..316
                     /gene="LOC144113470"
                     /coded_by="XM_077646562.1:<1..951"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 2 bases in 2 codons"
                     /db_xref="GeneID:144113470"
ORIGIN      
        1 hiviillhgl vpekieitgi edlldysqpg argallkacl vgsgvveids knklseqvla
       61 rhsggielqs wskmplgsgl gtssilaaai vaalwtavgq tfdkxavihc vlhveqlltt
      121 gggwqdqvgg mtgrlvhgss qphlplhvdv exsatillly tgkvrlaknl lqtvirnwyt
      181 retkvvscfk ellhlcktsv kesflqgdle asgkwldhyw qlkrilavgc epsfvarlmg
      241 llkpyvhgql llgaggggfl calakescqa gfiqrlldrs qgmpkvtihe vevdmtglrl
      301 sigteniqla ssmhpc
//
LOCUS       XP_077502699             281 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113480, partial [Amblyomma
            americanum].
ACCESSION   XP_077502699
VERSION     XP_077502699.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646573.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         <1..281
                     /product="uncharacterized protein LOC144113480"
     Region          <103..211
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          106..129
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320343"
     Region          145..168
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320343"
     Region          179..204
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320343"
     CDS             1..281
                     /gene="LOC144113480"
                     /coded_by="XM_077646573.1:<1..846"
                     /db_xref="GeneID:144113480"
ORIGIN      
        1 vssalahllv salllpstcv ailagvtrdp glchfqwall vvclvaslls faclaasqgl
       61 alrcsrgavl aaalaswava atvalgqrgl glgptfcgga paasnvplqa avaalcillp
      121 vlltvlgfgv alararrrep dgaalellks hvavylltla mwvpgvalaa vslsrqvpph
      181 lvdvvwwlal shsclysyvy aathrtfrva fnklffyccc kshvtfsrps reqrigpqgs
      241 gvglrvhiip amniysakkd ssqaavkhcv qgkglhcsyd l
//
LOCUS       XP_077502711             148 aa            linear   INV 25-NOV-2025
DEFINITION  la-related protein 6-like [Amblyomma americanum].
ACCESSION   XP_077502711
VERSION     XP_077502711.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646585.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="la-related protein 6-like"
                     /calculated_mol_wt=16812
     Region          69..143
                     /region_name="LAM"
                     /note="LA motif RNA-binding domain; cl02755"
                     /db_xref="CDD:470666"
     Site            order(73,76..77,82,85..86,88,109..111)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:153396"
     CDS             1..148
                     /gene="LOC144113490"
                     /coded_by="XM_077646585.1:1..447"
                     /db_xref="GeneID:144113490"
ORIGIN      
        1 msgegrsdns sasgaaegap maegdgmawe ervnrslrwd deecanlqds gylscyngqs
       61 reeeeieyri vtelesyfsd egldrnkflq nqvrrnrggf vslklmasfr klknltkdwe
      121 vvkkavlwss qlelnakktk vrrrlplp
//
LOCUS       XP_077502721             359 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113499 [Amblyomma americanum].
ACCESSION   XP_077502721
VERSION     XP_077502721.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646595.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 41% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..359
                     /product="uncharacterized protein LOC144113499"
                     /calculated_mol_wt=38788
     CDS             1..359
                     /gene="LOC144113499"
                     /coded_by="XM_077646595.1:1..1080"
                     /db_xref="GeneID:144113499"
ORIGIN      
        1 mslysqgvfa nlvvallvag ttchklhylk hlqptvnavp afyhttiahr plvlpaplva
       61 prplvqpllv qqrplppppp ihstvviqta prpappptqv lvqpllqprl vapppvlvpt
      121 rlapppvvvt hhhhhplvvs gvvaaapprs llvhapapaa lpappppppp ppavqplfvv
      181 qeprpalaav plqprlvhha vvptkldeap prvvhvvhqh tklvqeappp papavlpapv
      241 ptalvqplat vtpllepprq vqvirevirp tyvierlskk qrkfrkikml kncsllkklk
      301 sqlassaesy hlydphghyd ddahvdsysh vhvpypggsa avevyrsihg hgfakrngr
//
LOCUS       XP_077502734             611 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113506 [Amblyomma americanum].
ACCESSION   XP_077502734
VERSION     XP_077502734.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646608.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..611
                     /product="uncharacterized protein LOC144113506"
                     /calculated_mol_wt=66047
     Region          <297..562
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..611
                     /gene="LOC144113506"
                     /coded_by="XM_077646608.1:1..1836"
                     /db_xref="GeneID:144113506"
ORIGIN      
        1 mrdarekqgh lgwgpqartl lrrsggsqwv dgilrtadsa nrrrraerde raasrakpvl
       61 lgddvkdgat dgasepggav qarrksaqtc ccgdksgfra pninkddvtg ivvaiggrfp
      121 clhiglygls divryfvald faqissgang evdvhsrpyl cdpyytprpn nrfglewmdd
      181 vlffnlttia nggiefgagp mkvgaeyepa lrlvelryda wpagnstsrf slestaflvv
      241 slsselrvss kamisdlllv rksemvrngc rplqpprqql qpqhpqhhlq qqaqhqgpgs
      301 griicqqrsp llatptlppq lqaewlklfe lrdgirssaa grlggeaapm ppgllpqqfp
      361 ppalpgapvr ppalgapfpr pidskpltad ypggawrpph lqpenqqllq lshqlpqpic
      421 pgawctarql tclatashmr qgpmfqgiqq nseafpplip dprgvpllph palsefpyga
      481 gllptpefpp pllpygpvlt qppphywlsg pppilnalpp mrpsydpmpa gyqdwighar
      541 nnlprqphaa asgldveqqp epdmevaiqv gisvlesdde delfavggag pteakpdsfl
      601 apseanaasp p
//
LOCUS       XP_077502745            1515 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113513 [Amblyomma americanum].
ACCESSION   XP_077502745
VERSION     XP_077502745.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646619.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1515
                     /product="uncharacterized protein LOC144113513"
                     /calculated_mol_wt=170277
     Region          55..174
                     /region_name="Transglut_N"
                     /note="Transglutaminase family; pfam00868"
                     /db_xref="CDD:459971"
     Region          326..414
                     /region_name="TGc"
                     /note="Transglutaminase/protease-like homologues;
                     smart00460"
                     /db_xref="CDD:214673"
     Region          540..640
                     /region_name="Transglut_C"
                     /note="Transglutaminase family, C-terminal ig like domain;
                     pfam00927"
                     /db_xref="CDD:460002"
     Region          653..749
                     /region_name="Transglut_C"
                     /note="Transglutaminase family, C-terminal ig like domain;
                     pfam00927"
                     /db_xref="CDD:460002"
     Region          825..949
                     /region_name="Transglut_N"
                     /note="Transglutaminase family; pfam00868"
                     /db_xref="CDD:459971"
     Region          1097..1188
                     /region_name="TGc"
                     /note="Transglutaminase/protease-like homologues;
                     smart00460"
                     /db_xref="CDD:214673"
     Region          1304..1393
                     /region_name="Transglut_C"
                     /note="Transglutaminase family, C-terminal ig like domain;
                     pfam00927"
                     /db_xref="CDD:460002"
     CDS             1..1515
                     /gene="LOC144113513"
                     /coded_by="XM_077646619.1:294..4841"
                     /db_xref="GeneID:144113513"
ORIGIN      
        1 msghgrrhlt dpasdayrah maavlermrk eyerkqreea errgkhrgda ikvddvemya
       61 kdnakqhhte mfdnlessda pliirrgqsf flairfkrey qpgtdvinld fsigpnpems
      121 kgtfvslrvp dtkgdftrpp qswdvrvthh dravlslqvf vpvgvsvglw rmavlsrsrv
      181 dpeardkfrh pldiyvlfnp wckddlvymd nedwrreyvl ndvgkiymgs fkqpegrqwi
      241 fgqfgdmvlp acslildksn ltyaaranpv kvvrsisamv nsaddggvvt gnwsgnyddg
      301 tapwmwtgss ailekyvksg gtsvnygqcw vfsgvcntvc ralgipcrpv tcyasahdtd
      361 esitidryfd kegdelkkys rdsiwnfhvw nevwmarkdl ppgyggwqai datpqetsdg
      421 yyqvgpcsvi avkkgeigym ydspfvfaev nadiihwked pesafgwtrs ktltyhvglg
      481 vmtkkpgvki qegmadaedi tesyknqegt eeermavlna arsgglsvlf dlpsearqdv
      541 slklqdidsv migepfsvtv smrnesneqr tvnavlcsss vyytgilark ikrdkatfvl
      601 qpkdeevlsl tvqpeeyldk lvdyamvkiy vianvqetrq twseeddfti tkpklnieve
      661 gpvkvnqpfr lvmrftnpln vplqdctlsv egpgvttahi vhlsnvapha dwvhretfvp
      721 lrtgprsima vfncrelfni egstqtmvae ypcprcnqke rprqrstmdt sdeailemlr
      781 qrmlansnfl qymeiviesl rsgaefermp rlmahtggtn plqvktldyh ipantrhhkt
      841 dkflltqssd atlvvrrgap vtisiqfdrq ydgsrdqvfl ilntglepte sdgsrvrlev
      901 ptsrgsffsv dnspysiclv lteknsvtvk vmlppcaavg lwrlevetrl rgstdssdkc
      961 lfkvketfyv lfnpwcpedl vympqeellk eyifndtgkl yqgnfdyptg krwffgqftd
     1021 vvlptcnylf elgnvsvsdr sspikvaral aalvdskden gvlvgswdnn ygdgvsptaw
     1081 tssvtileqy mhtggapvky gqcwvysglv ttlcralgip crsitnfssa hdtdgnllie
     1141 rffdennnii agksedsiwn fhlwnelwmm rpdlpgdygg wqvidatpqt isdglfrvgp
     1201 cpvsaiyngh leldydtrfv yaevnadlvs wkrdpatgqf rqsnvrgyne aeaagmliyt
     1261 kkigemteht aedaedltkl ykgvkvrpnk svmglsgapp pedvelkmee issvlagqpv
     1321 rlavtcsnqs kvprkvsltl sahsvfytgg eykllkkqaf nvevppgkte tvcltvtade
     1381 yqlklapqnv vklfahgtye gsgqgtwyrq tritianpti nvevhgptnv srpmeyclsf
     1441 dnplnvpltg csvtldipgi mqksevipvp dvpakgkfeh kcriipkrpg eksilavfns
     1501 reltdirgnk vvevk
//
LOCUS       XP_077502833             431 aa            linear   INV 25-NOV-2025
DEFINITION  regulator of chromosome condensation-like [Amblyomma americanum].
ACCESSION   XP_077502833
VERSION     XP_077502833.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646707.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..431
                     /product="regulator of chromosome condensation-like"
                     /calculated_mol_wt=45697
     Region          48..405
                     /region_name="ATS1"
                     /note="Alpha-tubulin suppressor ATS1 and related RCC1
                     domain-containing proteins [Cell cycle control, cell
                     division, chromosome partitioning, Cytoskeleton]; COG5184"
                     /db_xref="CDD:444065"
     CDS             1..431
                     /gene="LOC144113563"
                     /coded_by="XM_077646707.1:1..1296"
                     /db_xref="GeneID:144113563"
ORIGIN      
        1 mtrrkkksra vgpfrkrgkp aenapsyakr irveereevv verrkipgqv ytvgqgdvgq
       61 lgfgqevmek srpalvpnld nvvdvvaggm htlcltvdgk vwsfgcndeg alglqtssld
      121 gaettpglve lpepvaklsa gdshsaaltv tgrafmwgnf rdssgqmgli vegqaekepk
      181 lvatgiveia sgsdhvamlt adgnlvtrgc aeqgqlgrva rmfasrggrr sstyllepgt
      241 vrlqrpkgck ssafdriwtg ayttyarlqe tgaiygfgln nyhqlgpskg dailhmpell
      301 dscagrrwka vsggqhhtlr lskegsvfsm grkeygrlgl gaanqpgnnd qetpgqvpgl
      361 sncteiscge avsfavdgeg slhswgfgtn gqlgrgnded sfeptvvagr ladrkvlvvs
      421 gggqhtvila s
//
LOCUS       XP_077502884             154 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113589 [Amblyomma americanum].
ACCESSION   XP_077502884
VERSION     XP_077502884.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646758.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 60% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..154
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..154
                     /product="uncharacterized protein LOC144113589"
                     /calculated_mol_wt=16665
     Region          55..149
                     /region_name="DUF3421"
                     /note="Protein of unknown function (DUF3421); pfam11901"
                     /db_xref="CDD:463390"
     CDS             1..154
                     /gene="LOC144113589"
                     /coded_by="XM_077646758.1:1..465"
                     /db_xref="GeneID:144113589"
ORIGIN      
        1 mrsrsrdnfl wlcgrsyere psayrvshyr ppchwvlchg erippnvvps gedkggsaif
       61 maravhegdv ipdkvvpsrg fcyvlvsdga slawlpssgg dvpsgaiqgg vtlsgeplyv
      121 grgrhegnlt igkfhpsrkt vhvlfggvey gysq
//
LOCUS       XP_077502894             192 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113596 [Amblyomma americanum].
ACCESSION   XP_077502894
VERSION     XP_077502894.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646768.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..192
                     /product="uncharacterized protein LOC144113596"
                     /calculated_mol_wt=21914
     Region          <80..122
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     CDS             1..192
                     /gene="LOC144113596"
                     /coded_by="XM_077646768.1:780..1358"
                     /db_xref="GeneID:144113596"
ORIGIN      
        1 mvtgiksdyv vnlvnwpqta ddlvlhyelr lqtlpegwer lrlssqpqlk sliasapdtg
       61 idnlfiapea lrpfvsscph vlqrwrlplm rrefllsklg eeplvrtsee skellieamr
      121 yhlmpappls rwliemqtpm hdllepydpl lntaltrrss llmspsisls wlqstalvrt
      181 tiiknppnni wa
//
LOCUS       XP_077502945             270 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113634 [Amblyomma americanum].
ACCESSION   XP_077502945
VERSION     XP_077502945.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646819.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 41% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..270
                     /product="uncharacterized protein LOC144113634"
                     /calculated_mol_wt=28786
     CDS             1..270
                     /gene="LOC144113634"
                     /coded_by="XM_077646819.1:1..813"
                     /db_xref="GeneID:144113634"
ORIGIN      
        1 maaiggavee plttakrasl nlihgreavt kkrgtaawcs tpsqkwprrk arlkilrcps
       61 arstdgskia fatgpaarss cvplvpscls svsygsykpt skramnflrg aaasceqlir
      121 klvprplnvl giqggdyard dlhrlesnsr takvrkkqap tslssvapcs hvqlpdvhar
      181 lfpcslhstd dvtpqpdqaa avvqpdcllg sgtfppkwlq eindqgqhsi rftqpaacaa
      241 saastnsrkd pmdpsgceds gqhrgmdtys
//
LOCUS       XP_077502954             167 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113641 [Amblyomma americanum].
ACCESSION   XP_077502954
VERSION     XP_077502954.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646828.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 21% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..167
                     /product="uncharacterized protein LOC144113641"
                     /calculated_mol_wt=17721
     CDS             1..167
                     /gene="LOC144113641"
                     /coded_by="XM_077646828.1:14..517"
                     /db_xref="GeneID:144113641"
ORIGIN      
        1 msspspgese dttafgfaev igrnirrgkk tgitprdfdl sslgspslia avpkfpelps
       61 phkvtrasae dsnskelrts hqkfpevlpt lpssahsekv prlsippnke keptngpdld
      121 slvpayspva nggeescgid qrspqqisrh asrsspehae dstglcq
//
LOCUS       XP_077502964              78 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113646 [Amblyomma americanum].
ACCESSION   XP_077502964
VERSION     XP_077502964.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646838.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 32% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..78
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..78
                     /product="uncharacterized protein LOC144113646"
                     /calculated_mol_wt=8122
     Region          8..>78
                     /region_name="DUF3421"
                     /note="Protein of unknown function (DUF3421); pfam11901"
                     /db_xref="CDD:463390"
     CDS             1..78
                     /gene="LOC144113646"
                     /coded_by="XM_077646838.1:1..237"
                     /db_xref="GeneID:144113646"
ORIGIN      
        1 mardarrdlv pgkvvpspdc cyvsydgteh shqeyqalvt ngtpmawvpa vggsvptgai
       61 qggscscgep lyigrtfh
//
LOCUS       XP_077502979            1030 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113650 [Amblyomma americanum].
ACCESSION   XP_077502979
VERSION     XP_077502979.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646853.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1030
                     /product="uncharacterized protein LOC144113650"
                     /calculated_mol_wt=115682
     Region          8..97
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          <321..>422
                     /region_name="transpos_IS110"
                     /note="IS110 family transposase; NF033542"
                     /db_xref="CDD:468073"
     Region          <429..614
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..1030
                     /gene="LOC144113650"
                     /coded_by="XM_077646853.1:1..3093"
                     /db_xref="GeneID:144113650"
ORIGIN      
        1 mtcilgkcfa pfcntgyksc kekyslfkpp ndearleawr raiprkdril qrtdrvcekh
       61 fashfilktw saeidshvlm sgtrraglsk davpsffega psylskkiks prkhvkrlal
      121 pvaasvsdrn sdsssalmsa snidvfpads metssddnac vakssendss hssfeqlfsg
      181 vscislpqps waahlinvag vrdvvfidaa vahrtsdgss vlfnrkavhi ksdmtvqvyi
      241 fdklidptav gispvatspl evqtmlkvvd ntdvcgggps lkdfpdvspe cafvdsqknw
      301 rhnkcllvts rgaicrlcsg ladtlrihad rraarakqqi pfkrfrllvg pvqqqklsal
      361 rrarsalqrs rarlakrnkl llgqlqaamk qltdlheqdi keklkgldip paqlllleec
      421 iaaakcasks srrytddwil lclllhirsp atysflrnnd ilplpcvttm rkyismvgqk
      481 cgfdenffka lkikvaakta fqrrgilild eiqvrkkvav nsqtmtytgl vdhgeknnqs
      541 neiaghglvf afapfgetyl qpvavfaskg ptkgtllsql liqcilenag lfvdgvvcdg
      601 astnramwkq lgitgslghv rnsfehpmdr srnvylpsdt phlfkctrnr lldkkvlkrd
      661 gklirwscyd alfvadsknk telkvcpkit yshinpsrml rmrvklatqi fsnsvakgil
      721 fyakrgaprl tnveptveft lflndlfdal nrrfpaeglk leckdfgvle sasewldswe
      781 qqvvsgeihk ylflrqaggq nehptfptfl qlyrmlslys lvkppkfgnc tvldkrqaav
      841 vtladiraaq hagklhelkq rldglidden wecddvfdfd dpdtdatvvd civyyvtgfv
      901 srklrnvttc svckgalegh kglanvpead lvnckttgwl thpnmhlfdl fkyteerfak
      961 yaddrdaydt ttnavlenfh ftfpcgthke emlakllhyy iclrmrqycr qlknkhekks
     1021 qdlrkmsklv
//
LOCUS       XP_077502993             795 aa            linear   INV 25-NOV-2025
DEFINITION  kinesin-like protein KIF20A [Amblyomma americanum].
ACCESSION   XP_077502993
VERSION     XP_077502993.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646867.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 42% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..795
                     /product="kinesin-like protein KIF20A"
                     /calculated_mol_wt=84641
     Region          301..700
                     /region_name="Motor_domain"
                     /note="Myosin and Kinesin motor domain; cl22853"
                     /db_xref="CDD:473979"
     Site            order(308..309,383..390,563..564,605..610)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:276812"
     CDS             1..795
                     /gene="LOC144113654"
                     /coded_by="XM_077646867.1:1..2388"
                     /db_xref="GeneID:144113654"
ORIGIN      
        1 madrapsddq sqpggsslcg vnpavvseqs sdnllpsgtp ldphpayhsg galslkppvv
       61 lespkatrle lstdadamkv heeivtaild kenktgpaar gvllplgdaa naaldvssgp
      121 pgrspgggsr pdhlphkfll tsvvasadvg sevaalikan idrevegigt vtlqpsrral
      181 mvasrdpael drlkaaitri eatrasinvk cigqedsyqi wadppvwpsq drnnyqfsdm
      241 eskkptagvf prprahqgpg nqlrrwgtes slmsasrvhr eqgdgtssll ettrtvptad
      301 vksfvrirpr fsgalfkepa feavcdttvv ttttttecnt qkrfrfhkvm pqrstqeevh
      361 aavmtgalqs ylkgghvlvl ccgptgagkt htlegnyrdp gilpralsil frprgpevsg
      421 aagvrpnlfs dvkplsaaea eellgykgkl lqekgarapp dfaafalddq rcalestnst
      481 gerqrkvglw vssfevyrdg iydlllpsse arnkepgkrr vplkigedga krafvkglle
      541 vpvhsadeah rllclaranq ttaetqlggr avrshlvltv rmatsaapgd ddqgswgvsv
      601 ltfielvsse kpakaggdgs llreaghina sllvlgrcme avglketskk gpvpfrdsrl
      661 tqviqpfftt gaevalltni spalstfees lnalkfaats vneaplplvp lmprqercrr
      721 aarrltevwl thvrggdase pgstlepdag aaplnaaqtd elfetiaalq agarqhsaps
      781 athwcgapgc cgrsa
//
LOCUS       XP_077503001             439 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113661 [Amblyomma americanum].
ACCESSION   XP_077503001
VERSION     XP_077503001.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646875.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..439
                     /product="uncharacterized protein LOC144113661"
                     /calculated_mol_wt=49466
     Region          <2..125
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..439
                     /gene="LOC144113661"
                     /coded_by="XM_077646875.1:1..1320"
                     /db_xref="GeneID:144113661"
ORIGIN      
        1 mrssswtkat vegalklrla cgsrgyeylr etgwplpaer tlqshiedik malvatlqve
       61 elyaavlide illtpgldyd nstrtviast fdaskllell fdvirrseqi gicvdcvtsd
      121 mgagnqavrl dyikrlcetd ekhdlklaph lkpkylspnh yekmnfvhqw falmssrtsk
      181 malsdlcpek gteavnfksv ievftklqif dagksssvwk pvqsgviitt ctalrlrelm
      241 ikerglkyll lsrfvqdale nlfntvrlks pvprardfky alrmiilaqf fqpskkgsyq
      301 iddgvqlaqf vstkpnvlkp leeeveqndv slpaeeqecl hylagyitrk virknklcgv
      361 csaalkgsse dsgrllkmkn yvegkeslci psqpvtgllq eaelyfrsnk knitevhlis
      421 llsklaegei snkqfprtr
//
LOCUS       XP_077503029             117 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113672 [Amblyomma americanum].
ACCESSION   XP_077503029
VERSION     XP_077503029.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646903.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 50% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..117
                     /product="uncharacterized protein LOC144113672"
                     /calculated_mol_wt=12529
     CDS             1..117
                     /gene="LOC144113672"
                     /coded_by="XM_077646903.1:79..432"
                     /db_xref="GeneID:144113672"
ORIGIN      
        1 mkrlsavhvl npehrfldas gepmlslfaa dryhvarrrg idpsaplflc sgksrvkvat
       61 iimplgslsa atqiiasvaa fdppeaaqrp vkcsgvlaig ierhvhnthl ttqntrp
//
LOCUS       XP_077503051             338 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113685 [Amblyomma americanum].
ACCESSION   XP_077503051
VERSION     XP_077503051.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646925.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 21% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..338
                     /product="uncharacterized protein LOC144113685"
                     /calculated_mol_wt=34063
     Region          <22..255
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..338
                     /gene="LOC144113685"
                     /coded_by="XM_077646925.1:1..1017"
                     /db_xref="GeneID:144113685"
ORIGIN      
        1 mqspawsrka ntttstsegt tddspasfgl ngqataapis cttpsdlpaw dpattasppc
       61 kartnsvqat ddspasfgln gqataapisc ttpsdlpawd patiasspgk artnsvqatd
      121 dspasfglng qataapisct tpsdlpawdp atiasspgka rtnsvqatdd spasfglngq
      181 ataapisctt psdlpawdpa tiasspgkar tnsvqatdds pasfglngqa taapiscttp
      241 sdlpawdpat iasspckart nsvqateate saegaestqd ktivrkpsfa vscymrrnhl
      301 qqpktpnnag heaalqplas ayirgiahnr lapasnlg
//
LOCUS       XP_077503064             929 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113689 [Amblyomma americanum].
ACCESSION   XP_077503064
VERSION     XP_077503064.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646938.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..929
                     /product="uncharacterized protein LOC144113689"
                     /calculated_mol_wt=96582
     Region          82..>274
                     /region_name="Chi1"
                     /note="Chitinase [Carbohydrate transport and metabolism];
                     COG3469"
                     /db_xref="CDD:442692"
     Region          193..>401
                     /region_name="Chi1"
                     /note="Chitinase [Carbohydrate transport and metabolism];
                     COG3469"
                     /db_xref="CDD:442692"
     CDS             1..929
                     /gene="LOC144113689"
                     /coded_by="XM_077646938.1:85..2874"
                     /db_xref="GeneID:144113689"
ORIGIN      
        1 mglrhrrlps lvrhqrpghg gphqlhhplq sprmglrhrr lpslvrhqrp ghggphqlhh
       61 pllcprmglr hpsvkthpen mddspassai nglataapts cttpsyvpaw dsatddspas
      121 saingqataa ptscttpsyv pawdsatdds passaingqa taahnscttp sylpawdsvt
      181 ddspassain gqataaptsc ttlsnlpawd satddspass aingldtaap tscttpsyvp
      241 awdsatddsp assainglat aaptscttps yvpawdsatd dspassaing lataaptsct
      301 tpsyvpawds atddspassa ingldtaapt scttpsyvpa wdsatddspa ssainglata
      361 aptscttpsy vpawdsatdd spassaingq ataaptsctt psyvpawdsa tddssassai
      421 igqataapts cttpsylpaw dpatiasspc karthsvqat eategaegae skqdktivrk
      481 psfavsyymr rnhlqqpktp nnaghvarlk rmvrnmlkln kelavalaaa rtevtkmeen
      541 dlrraeahqv lpqiissmee gsaehggtyg qhiralrllh vlltpgaevt ltdsndgsee
      601 ggpcqqenva qgpeecgfsv eedkkhkkdp evprrstavr darlihrrss ppsacnlpai
      661 pesyeefscq slceaqqpea dgtprqgaqp qelqggslpa gllpfsvpqq qqaglqnsar
      721 lphgdagsqq aadsssrrqq kratsmvglm dtdpirrqaa gavpmegdpt gpasikprsm
      781 enevsvpstv gpenghlrta qlrrpassdq ddtvtttpvs sgrylekvpa rssagasasa
      841 gtdeteleaa gpsaaataga geiagesslq iknvrnkrqg aprrapalev lpdtceqdip
      901 ltasgrplrq aavgknykep gggrktrrr
//
LOCUS       XP_077503073             932 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113694 [Amblyomma americanum].
ACCESSION   XP_077503073
VERSION     XP_077503073.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646947.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..932
                     /product="uncharacterized protein LOC144113694"
                     /calculated_mol_wt=105058
     Region          13..85
                     /region_name="Propep_M14"
                     /note="Carboxypeptidase activation peptide; pfam02244"
                     /db_xref="CDD:460505"
     Region          108..401
                     /region_name="M14_CP_A-B_like"
                     /note="Peptidase M14 carboxypeptidase subfamily A/B-like;
                     cd03860"
                     /db_xref="CDD:349433"
     Site            order(164,167,222,238..239,291,344,346,367)
                     /site_type="active"
                     /db_xref="CDD:349433"
     Region          535..607
                     /region_name="Propep_M14"
                     /note="Carboxypeptidase activation peptide; pfam02244"
                     /db_xref="CDD:460505"
     Region          630..925
                     /region_name="M14_CP_A-B_like"
                     /note="Peptidase M14 carboxypeptidase subfamily A/B-like;
                     cd03860"
                     /db_xref="CDD:349433"
     Site            order(688,691,746,763..764,816,868,870,891)
                     /site_type="active"
                     /db_xref="CDD:349433"
     CDS             1..932
                     /gene="LOC144113694"
                     /coded_by="XM_077646947.1:1..2799"
                     /db_xref="GeneID:144113694"
ORIGIN      
        1 mrrallsrvy klfsvyignd tatldvisal renpevdfwn gptlnrnvsf lippglveqi
       61 qnllknagie yqiltddiqr widredeenr pdvfleqrdi tnyafdkyhr ideitgyldl
      121 laqkyshiat vrnigmtqeg rpikgvilst gssrpvlwfd agiharewva pstllyflhk
      181 mttgygsdst vtallqtfdf yifpvinpdg yaytftsdrl wrknrsggrr gcrgvdpnrn
      241 fatvfggagt sghpcsdiyr gaqafseaes rairdailel gsrtkgyvtv hsysqllmvp
      301 yghgrgtytr dyadqiasar avsraiqirs gvyyqvgtis sllgssagss sdwvydgaki
      361 kyslavelrd kgrygfllpn flivptadea aegfkafgkf iarrelnrwr pratdsrrpr
      421 apsevdkplr avsalsfhgq gegtcpwaps sargslsgds pgaqtsrrql rvvaaadgga
      481 ssfpffpvrk lrqdyfqear kmhrsimrvl lswllfalfs acnarvsfqg hklvraylns
      541 mssvellrnl qtnnthldfw depvapdqni tirlppseas mfqkhlelsg iqytvltdnl
      601 qewidrerid nqrdedlnpr dagtfrtdmy htldeiygfl dssaqkfpsi vtrvtigktd
      661 egasirgvki ssggnssrpa mwidsgihar ewistasaly vidrllkgyn dsdditklvd
      721 tfdwyifpvi nadgykytwt thrlwrknrv rnvaslcrgv dpnrnfdvrf glagssanpc
      781 aenyagtfpf seaesraird ginnlkdrlk ayinlhsysq mvmipygysk gyttdyrsqy
      841 ealeklvtai rkrnsafyrh gtagqtlyit sgaaldwvyd kakvkhtfvi elrdrglfgf
      901 ilprefitpt gdelfsglka lafhiiksel ds
//
LOCUS       XP_077503086             339 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113699 [Amblyomma americanum].
ACCESSION   XP_077503086
VERSION     XP_077503086.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646960.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..339
                     /product="uncharacterized protein LOC144113699"
                     /calculated_mol_wt=36215
     Region          <82..>222
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     CDS             1..339
                     /gene="LOC144113699"
                     /coded_by="XM_077646960.1:136..1155"
                     /db_xref="GeneID:144113699"
ORIGIN      
        1 mdgsssdddv lpkklleerr knatllrrlk qvrkekaelq arhdrvedif lnkfeswlaa
       61 rchlclkehs sctcrtersg asplqqvrec spyseltapl aaattlqpsp lgdfknmeas
      121 kstglvmpqn lgalaessgq astsmapgpl qnhpggvlgv sgqastsmas aplqdhpgal
      181 vevsgqasss malapsqnlg ilsgvsrqas tsmapaplqd hpgalvevsg qvhlgngmyv
      241 saerwrwlls rgrdslfckd takllwgvsn lrgksvtgaa crrflkagat qkpgltpekl
      301 aavgnafday iianptaedq kawrgkmnrf ladmlrdik
//
LOCUS       XP_077503125             186 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113718 [Amblyomma americanum].
ACCESSION   XP_077503125
VERSION     XP_077503125.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646999.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..186
                     /product="uncharacterized protein LOC144113718"
                     /calculated_mol_wt=21427
     CDS             1..186
                     /gene="LOC144113718"
                     /coded_by="XM_077646999.1:175..735"
                     /db_xref="GeneID:144113718"
ORIGIN      
        1 mamssavlim mltlhqlyfm tllmswisfh nmllsaavih yrarcqrirs isnsrtplta
       61 gwsvhevavm ygleslappp krrmwmknrs sdwwdnivcs nfddgewken frmsranfce
      121 lvaalapfms pecnyvrepv pvdkriaial yklascceyr vvanlfgihk stvqkflylf
      181 citkvl
//
LOCUS       XP_077503136             261 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113725 [Amblyomma americanum].
ACCESSION   XP_077503136
VERSION     XP_077503136.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647010.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..261
                     /product="uncharacterized protein LOC144113725"
                     /calculated_mol_wt=28892
     Region          16..112
                     /region_name="THAP"
                     /note="The THAP domain is a putative DNA-binding domain
                     (DBD) and probably also binds a zinc ion; smart00980"
                     /db_xref="CDD:214951"
     CDS             1..261
                     /gene="LOC144113725"
                     /coded_by="XM_077647010.1:1..786"
                     /db_xref="GeneID:144113725"
ORIGIN      
        1 mvptlracns agvivycavv gctsrtqska qklktgqadc rffkipkirl hecekakqls
       61 yrrrrewlar inrsetdanp dkykvcalhf vsgrpsklfd dsspdwtpsl hlgcalkkpd
      121 qsryhrrkar shrqfssaga vggsardepa tsegasmcde gteplvcsla dtacqtdlga
      181 adirgledqi sglrqqlqas eeascstsfi eealhgcder vafytglptf vmlhslfklv
      241 kdhishsrkh sltqlqemvl f
//
LOCUS       XP_077503172             160 aa            linear   INV 25-NOV-2025
DEFINITION  cytoplasmic dynein 2 intermediate chain 2-like [Amblyomma
            americanum].
ACCESSION   XP_077503172
VERSION     XP_077503172.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647046.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..160
                     /product="cytoplasmic dynein 2 intermediate chain 2-like"
                     /calculated_mol_wt=17933
     CDS             1..160
                     /gene="LOC144113746"
                     /coded_by="XM_077647046.1:5..487"
                     /db_xref="GeneID:144113746"
ORIGIN      
        1 mhdeprltag eilsmfsael vhfdefspsg kecrdtchts vqtepravre agvqrrhwcd
       61 aevqaspevv asvepraydw pslatfmrqa yprmtqeler asrssafrgy rlladtsegs
      121 asrlyrdtaf prelhctdas wnstgsivaf syplalrncp
//
LOCUS       XP_077503184             160 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113750 [Amblyomma americanum].
ACCESSION   XP_077503184
VERSION     XP_077503184.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647058.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..160
                     /product="uncharacterized protein LOC144113750"
                     /calculated_mol_wt=17804
     Region          <89..132
                     /region_name="Dimer_Tnp_hAT"
                     /note="hAT family C-terminal dimerization region;
                     pfam05699"
                     /db_xref="CDD:399013"
     CDS             1..160
                     /gene="LOC144113750"
                     /coded_by="XM_077647058.1:1..483"
                     /db_xref="GeneID:144113750"
ORIGIN      
        1 meqlvkledi lnsaaggsry tadeleaalg dhavdfdlcr lsaqfmllsa llngadsatt
       61 lgileilqre sqnirelmdq vvryvqlrfs ipasvaseer cfsalrrikt ylrnrttqkr
      121 sshllilhih kertallssd diirefvsrt geraatfgpa
//
LOCUS       XP_077503197            1077 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113760 [Amblyomma americanum].
ACCESSION   XP_077503197
VERSION     XP_077503197.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647071.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 12% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1077
                     /product="uncharacterized protein LOC144113760"
                     /calculated_mol_wt=119718
     Region          <317..>352
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     Region          402..>441
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            416..418
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133137"
     Site            416
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133137"
     Region          617..676
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          693..789
                     /region_name="rve"
                     /note="Integrase core domain; pfam00665"
                     /db_xref="CDD:459897"
     CDS             1..1077
                     /gene="LOC144113760"
                     /coded_by="XM_077647071.1:1225..4458"
                     /db_xref="GeneID:144113760"
ORIGIN      
        1 mvrtrgmmas tadseteesg mtpktstdet snlgesrapp ggtaqvetgs elpgvaadie
       61 pdsyalrrle ldfelqrlkl eaeeriamrr aelefggarq sppvsdwsgq rqtgldpttq
      121 cakvlkglrl padsdvpmwf devermfaty nvpdssrvhl iiptltervr yllrglgdea
      181 crdyeavkaa vlselqlspp eylerfegas krkdetwaqf asrvktyfey ylrsrqassk
      241 eevvslivad rmkaslsaeg lehirlrege kwlrpdevar amqtfeqakg knrgvrqasa
      301 qpaskeqagp rsaslapsra ahdgkrqptc yvcraaghla kdcprviqkk ddnrrdaran
      361 avtiadpgtv rdapashsvn vlvaeikvpq reeirksrlq qvqldcagvp tvaildtgse
      421 vtvvredlvp nayrettgni rlvaafgnti eaklvslpig lrssalllep qkvriicalt
      481 nklaegvdcl lskedwells qgsqgeavav faetpksdat igqpgsefgt gsetvseaii
      541 letsaeappa elrdeevtda petlsqrekf raaqvsdesl rkawsdaksg kggmfisegl
      601 lyhwdtiaga rvkqlvlpce rrqevlllah eslwgghlgp rktrarikys fywpgleqev
      661 kqhcktchgc qirsdrsird rvpitpltrp ehpfqvvnvd iigpidvpsa rghkyalclv
      721 dlctrwpevi clrsltarat cdalltvfsr tgvpevicsd qgtnftsqlt qaflarlgcs
      781 prfstpehpe sngtverwnr vlknmifhvm gkdaknwdkl ipfvlwayre vphdttgvap
      841 frllygrnpt gplailqrtw tgeirvpstl reapakylqq lreqmeiaae vagltatarq
      901 gsyaqaynra trvksfnvgd qvllfdtdrg gklspkwlgp fpvvgrerqn syridtgark
      961 tsvvhanrlr pyyarvshvg vvfdedeefg nveyapcaea giqgqtpiea tkmahlkeeq
     1021 rseiaelfar ypevfsetpg iaevgthriv ledgyrpkra fpyrvpellr keqrske
//
LOCUS       XP_077503216             235 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113777 [Amblyomma americanum].
ACCESSION   XP_077503216
VERSION     XP_077503216.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647090.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 31% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..235
                     /product="uncharacterized protein LOC144113777"
                     /calculated_mol_wt=25457
     CDS             1..235
                     /gene="LOC144113777"
                     /coded_by="XM_077647090.1:1..708"
                     /db_xref="GeneID:144113777"
ORIGIN      
        1 mtrqalwsrt gtsqaesrah ergatvhgrl pplkwpelhl srlktrrrpc avhalntsfh
       61 fledqvrvsa agastnsaaa pfyeaggvyg gtplsllalp ascvpdvsak aalpaaavar
      121 vspepgpgsr delrrlvsep dlllasevdv dvgfvckkcq lvfpaeplvv ahqraacfaa
      181 gkppnvgadf kpflrlvqra wecrrcrdrl htarefkfhc dterhcgksk eaghn
//
LOCUS       XP_077503261             103 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113800 [Amblyomma americanum].
ACCESSION   XP_077503261
VERSION     XP_077503261.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647135.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..103
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..103
                     /product="uncharacterized protein LOC144113800"
                     /calculated_mol_wt=10019
     CDS             1..103
                     /gene="LOC144113800"
                     /coded_by="XM_077647135.1:137..448"
                     /db_xref="GeneID:144113800"
ORIGIN      
        1 mggggsghsp spataaccep arplvgespg aapasaasgp vcslpaagsy dsrllssypq
       61 latprfynag ygdqaaaaad yanlavdssa fyptlcdelg sky
//
LOCUS       XP_077503270             889 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113807 [Amblyomma americanum].
ACCESSION   XP_077503270
VERSION     XP_077503270.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647144.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..889
                     /product="uncharacterized protein LOC144113807"
                     /calculated_mol_wt=93071
     CDS             1..889
                     /gene="LOC144113807"
                     /coded_by="XM_077647144.1:277..2946"
                     /db_xref="GeneID:144113807"
ORIGIN      
        1 msadfdhnpy edptglmras qetlqtgrpp pvicatapsg prpqvkylkt rhlwpgsatr
       61 gktyrkyksn milsespkia gtypaevppe tnvsirgenm gldhsnlvav hggpapkapr
      121 igptphaett arslppiaas cisedecfgs rdrlliasre qrgtgkrsal shllnakryr
      181 ynstkvpltg tgasaqatpd ipapvaasav rdclpasnmc csaaspakqr aaakrsailr
      241 lfsgakgtys vhggpppkap rigptphaet tarslppiaa scisedeslg rrdrqliasr
      301 eqrgtgkrsa lphllnakky rynstkvplt gtgasaqatp dipapvaasa vrdclpasnm
      361 ccsaaspakq raaakrsail rlfsgakgty svhggpppka prigptphae ttarslppia
      421 ascisedesl grrdrqlias reqrgtgkrs alphllnakk yrynstkvpl tgtgasaqst
      481 pyipapvaas adrdclpasn mycsaaspak qraaakrsai lrlfngakgt ysvhggpppk
      541 aprigptpha ettarslppi aascisedes fgrrdrqlia sreqrgtgkr salshllnat
      601 syrynptkvp ltgkaasapa apdspapvaa savrdclpas nmgcpagspa kqraaaarsa
      661 ilrlfsgtkg tfsvhggpap kaprigptph aettarslpp iaascisenq clgrrnrqli
      721 asreqrgtgk rsalshllna kryrynstkv pltgtgasaq atpdipapva asavrdclpa
      781 snmccsaasp gkqraaatrs ailrlfsgak gtysvhggpa pkaprigptp haettarslp
      841 piaascisen qclgrrnrql iasreqrgtg krsalshlln akryryses
//
LOCUS       XP_077503282             151 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113817 [Amblyomma americanum].
ACCESSION   XP_077503282
VERSION     XP_077503282.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647156.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..151
                     /product="uncharacterized protein LOC144113817"
                     /calculated_mol_wt=17084
     CDS             1..151
                     /gene="LOC144113817"
                     /coded_by="XM_077647156.1:1..456"
                     /db_xref="GeneID:144113817"
ORIGIN      
        1 maaqipwvkg qdkdvlihnd kqgtlifstp dmdtvwkvtk iksihiddde yevtaylaph
       61 edcgrgvvhg idpkltieel teafgnprnp pilgvrkmgk sssaivifkh etvprwvycy
      121 dvplkcvlyk kryevcyrcg elghrsdvrp s
//
LOCUS       XP_077503294             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113825 [Amblyomma americanum].
ACCESSION   XP_077503294
VERSION     XP_077503294.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647168.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144113825"
                     /calculated_mol_wt=17915
     CDS             1..158
                     /gene="LOC144113825"
                     /coded_by="XM_077647168.1:1..477"
                     /db_xref="GeneID:144113825"
ORIGIN      
        1 mgerfhrqlk aalmaseqhc waeaspvmrl girtvfradi gcsaaelvys tmlrlpdefs
       61 asgqqqaris agdyafrlrd vmnklqatpp rqpserrthv rnefdscsqv flrndavrrp
      121 lqaphngslk vlhrgrktlt iemrgrrevv lldreksa
//
LOCUS       XP_077503304             242 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113834 [Amblyomma americanum].
ACCESSION   XP_077503304
VERSION     XP_077503304.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647178.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..242
                     /product="uncharacterized protein LOC144113834"
                     /calculated_mol_wt=27538
     CDS             1..242
                     /gene="LOC144113834"
                     /coded_by="XM_077647178.1:141..869"
                     /db_xref="GeneID:144113834"
ORIGIN      
        1 mqkcliwlit lcsvgepmkl dqralelayr rdvwlansll dliverlare psvsgaldpl
       61 nldevamdrg rifnvsvegl srlhrsadta lrlrnsftvt iegqlsfgnl siksmyhfkp
      121 isvleleghm dvlltgltvg ieisvkhevp vltqfkvvef gvadvtrftg atfalnwlgr
      181 ilttellrqy keyivitiet kaksfidsvl eniklpfrsk flqltnhark ahrerrvirr
      241 kh
//
LOCUS       XP_077503314             844 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 1-like [Amblyomma americanum].
ACCESSION   XP_077503314
VERSION     XP_077503314.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647188.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..844
                     /product="endothelin-converting enzyme 1-like"
                     /calculated_mol_wt=93604
     Region          225..837
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..844
                     /gene="LOC144113841"
                     /coded_by="XM_077647188.1:1..2535"
                     /db_xref="GeneID:144113841"
ORIGIN      
        1 mrkgglwknl rplsggsrvp pevtqnkral sadllmqrtk gvksskldvl qrsspaascn
       61 rdgkhvlkvl kasglsgcra tpensktpaq alagshpddl adhdfpfesv kktssatpls
      121 eppaveqqqp fgriilmrkk aviytailvv laltlgvsas ylhnkfvnah stqfpaaras
      181 kslllagngs fvgpdaiked ydtcsttacr ldgaylkgll swdldpcdnf yrfvcsrwkg
      241 gggvvkgita aaasdelshq leqsvhvliq rssspedfap lkalfyecmn trqldlddwn
      301 pmlellwlvs legfpfsstp rnstslwkia akvlkmtgae allsmravah ptkgdtgils
      361 vgvptmlapk gsdqahatqf ynstafacit alkkqrnptv yslevaafas rlemlvytft
      421 vlaglpsgri kpiktqpels aflvqvflns dsslysgsht elhiqspaff ealitlvrnt
      481 eqyivmnylg vrlmievaaf apssgrplvh tlvhhlygrp rpllprwrlc iraaekalpd
      541 lflhagklaf kthyaldmvq rllealrhql lhslsklail drdsgaraqa llnstrfrlf
      601 rpawlsnhek legymsalpr vvrgqslrsf ytlhshsfte slrrdeadrw qgsafdhdct
      661 fdgnvvyvpv llfnftlfsy dadqslqlpn sgvrivrcll qlllnsvnsg gsvspvasrv
      721 wwpdmsssll avaqlclqqy gvrqlgplel lqhtaalkpa mdlfqeqrri dvrfetfqty
      781 svfhlffvyy alalcednsa dntvasfltn vplwnnrlfq etfrcpvgsn mnpsrkcivw
      841 reth
//
LOCUS       XP_077503321             138 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113850 [Amblyomma americanum].
ACCESSION   XP_077503321
VERSION     XP_077503321.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647195.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..138
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..138
                     /product="uncharacterized protein LOC144113850"
                     /calculated_mol_wt=15780
     CDS             1..138
                     /gene="LOC144113850"
                     /coded_by="XM_077647195.1:1..417"
                     /db_xref="GeneID:144113850"
ORIGIN      
        1 meslidgvlf sfllllrgka aarlsyqril gekecdsdra qkkyefcagd fmrmlqlmei
       61 gtdnericqi rekyreclet ssretncdfk shalsrlqtl ttlltrdygi vcespdegds
      121 agrgnrrfrr mfattena
//
LOCUS       XP_077503342             352 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077503342
VERSION     XP_077503342.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647216.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 18% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..352
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=39011
     Region          7..337
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..352
                     /gene="LOC144113859"
                     /coded_by="XM_077647216.1:1..1059"
                     /db_xref="GeneID:144113859"
ORIGIN      
        1 mtdeyrgkyv prailldlep gtmdsvrsgp fgqlfrpdnf ifgqsgagnn wakghytega
       61 elvdsvldvv rkeaescdcl qgfqlahslg ggtgsgmgtl liskireeyp drimntfsvm
      121 pspkvsdtvv epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs
      181 vtmsgvttcl rfpgqlnadl rklavnmvpf prlhffmpgf apltsrgsqq yraltvpelt
      241 qqmfdaknmt aacdprhgry ltvatifrgr msmkevdeqm lnvqnknssy fvewipnnvk
      301 tavcdipprg lkmsatfign stdiqelfkr iseqftvdnp ngdkgpspsl ar
//
LOCUS       XP_077503355             396 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like [Amblyomma americanum].
ACCESSION   XP_077503355
VERSION     XP_077503355.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647229.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..396
                     /product="tubulin beta chain-like"
                     /calculated_mol_wt=44411
     Region          7..380
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..396
                     /gene="LOC144113866"
                     /coded_by="XM_077647229.1:1..1191"
                     /db_xref="GeneID:144113866"
ORIGIN      
        1 mtdeyrgkyv prailldlep gtmdsvrsgp fgqlfrpdnf ifgqsgagnn wakghytega
       61 elvdsvldvv rkeaescdcl qgfqlahslg ggtgsgmgtl liskireeyp drimntfsvm
      121 pspkvsdtvv epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs
      181 vtmsgvttcl rfpgqldadl rklavnmvpf prlhffmpgf apltsrgsqq yraltvpelt
      241 qqmfdaknmm aacdprhgry ltvatifrgr msmkevdeqm lnvqnknssy fvewipnnvk
      301 tavcdipprg lkmsatfign staiqelfkr iseqftamfr rkaflhwytk egmdemefte
      361 aesnmndlvs eyqqyqeata ddegefdded aladta
//
LOCUS       XP_077503369             397 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077503369
VERSION     XP_077503369.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647243.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..397
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=44478
     Region          7..378
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..397
                     /gene="LOC144113871"
                     /coded_by="XM_077647243.1:1..1194"
                     /db_xref="GeneID:144113871"
ORIGIN      
        1 mtdeyrgkyv prailldlei gtmdsvrsgp fsqlfrpdnf ifgqsgagnn wakghytega
       61 elvdsvldvv rkeaescdcl qgfqlahslg ggtgsgmgtl liskireeyp drimntfsvm
      121 pspkvsdtvv dsynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs
      181 vtmsgvttcl rfpgqlnadl rklavnmvpf prlhffmpgf apltsrgsqq yraltvpelt
      241 qqmfdaknmm aacdprhgry ltvatifrgr msmkevdeqm lnvqnknssy fvewipnnvk
      301 tavcdiplrg lkmsatfign staiqelfkr iseqftamfh rkaflhwytg egmdemefte
      361 aesnmndlvs eyqhneaads laresrsraq adltdkp
//
LOCUS       XP_077503381             396 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like [Amblyomma americanum].
ACCESSION   XP_077503381
VERSION     XP_077503381.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647255.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..396
                     /product="tubulin beta chain-like"
                     /calculated_mol_wt=44401
     Region          7..396
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..396
                     /gene="LOC144113879"
                     /coded_by="XM_077647255.1:1..1191"
                     /db_xref="GeneID:144113879"
ORIGIN      
        1 mtdeyrgkyv prailldlep gtmdsvrsgp fgqlfrpdnf ifgqsgagnn wakghytega
       61 elvdsvldvv rkeaescycl qgfqlahslg ggtgsgmgtl liskireeyp drimntfsvm
      121 pspkvsdtvv epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs
      181 vtmsgvttcl rfpgqlnadl rklavnmvpf prlhffmpgf apltsrgsqq ywaltvpelt
      241 qqmfdaknmm aacdprhgry ltvatifrgr msmkevdeqm lnvqnknssy fvewipnnvk
      301 tavcdipprg lkmsatfign staiqelfkr iseqftamfr rkaflhwytg egmdemefte
      361 aesnmndlvs eyqqyqeata dvegefdded aladta
//
LOCUS       XP_077503393             517 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like [Amblyomma americanum].
ACCESSION   XP_077503393
VERSION     XP_077503393.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647267.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 59% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..517
                     /product="tubulin beta chain-like"
                     /calculated_mol_wt=57644
     Region          67..>113
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          128..501
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..517
                     /gene="LOC144113886"
                     /coded_by="XM_077647267.1:1..1554"
                     /db_xref="GeneID:144113886"
ORIGIN      
        1 mvwgcvwvda tcdehgatav vrnphiaslt felpsgttpe gaekttiala ytnvgyiisf
       61 dmpnkycvpm cssnyktgnk vqvfplpkde qlykwlsaip rkdfvptann ktcpmwrphs
      121 slfggargky vprailldle pgtmdsvrsg pfgqlfrpdn fifgqsgagn nwakghyteg
      181 aelvdsvldv vrkeaescdc lqgfqlahsl gggtgsgmdt lliskireey pdrimntfsv
      241 mpspkvsdtv vepynatlsv hqlventdet ycidnealyd icfrtlkltt ptygdlnhlv
      301 svtmsgvttc lrfpgqlnad lrklavnmvp fprlhffmpg fapltsrgsq qyraltvpel
      361 tqqmfdaknm maacdprhgr yltvatifrg rmsmkevdeq mlnvqnknss yfvewipnnv
      421 ktavcdippr glkmsatfig nstaiqelfk riseqftamf rrkaffhwyt gegmdemeft
      481 eaesnmndlv seyqqyqeat addegesdde daladta
//
LOCUS       XP_077503405             448 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4B chain-like [Amblyomma americanum].
ACCESSION   XP_077503405
VERSION     XP_077503405.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647279.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 12% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..448
                     /product="tubulin beta-4B chain-like"
                     /calculated_mol_wt=50227
     Region          2..>44
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          59..432
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..448
                     /gene="LOC144113893"
                     /coded_by="XM_077647279.1:1..1347"
                     /db_xref="GeneID:144113893"
ORIGIN      
        1 mcssnyktgn kvqvfplpkd eqlykwlsai prkdfvptan nktcpmwrph sslfggdrgk
       61 yvprailldl epgtmdsvrs gpfgqlfrpd nfifgqsgag nnwakghyte gaelvdsvld
      121 vvrkeaescd clqgfqlahs lgggtgsgmg tlliskiree ypdrimntfs vmpspkvsdt
      181 vvepynatls vhqlventde tycidnealy dicfrtlklt tptygdlnhl vsvtmsgvtt
      241 clrfpgqlna dlrkqavnmv pfprlhffmp gfapltsrgs qqyraltvpe ltqqmfdakn
      301 mmaacdprhg ryltvatifr grmsmkevde qmlnvqnkns syfvewipnn vktavcdipp
      361 rglkmsatfi gnstaiqelf kriseqftam frrkaflhwy tgegmdemef teaesnmndl
      421 vseyqqyqea taddegefdd edaladta
//
LOCUS       XP_077503425             293 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like [Amblyomma americanum].
ACCESSION   XP_077503425
VERSION     XP_077503425.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647299.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..293
                     /product="tubulin beta chain-like"
                     /calculated_mol_wt=33035
     Region          <21..277
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..293
                     /gene="LOC144113909"
                     /coded_by="XM_077647299.1:1..882"
                     /db_xref="GeneID:144113909"
ORIGIN      
        1 mamsssgtgp kvriqahskl qvsdtvvepy natlsvhqlv entdetycid nealydicfr
       61 tlklttptyg dlnhlvsvtm sgvttclrfp gqlnadlrkl avnmvpfprl hffmpgfapl
      121 tsrgsqqyra ltvpeltqqm fdaknmmaac dprhgryltv atifrgrmsm kevdeqmlnv
      181 qnknssyfve wipnnvktav cdipprglkm sstfignsta iqelfkrise qftamfrrqa
      241 flhwytgegm demefteaes nmndlvseyq qyqeatadde gefddedala dta
//
LOCUS       XP_077503437             366 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like [Amblyomma americanum].
ACCESSION   XP_077503437
VERSION     XP_077503437.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647311.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..366
                     /product="tubulin beta chain-like"
                     /calculated_mol_wt=40810
     Region          <13..350
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..366
                     /gene="LOC144113917"
                     /coded_by="XM_077647311.1:1..1101"
                     /db_xref="GeneID:144113917"
ORIGIN      
        1 mqvayiravt fagqsgagnn wakghytega elvdsvldvv rkeaescdcl qgfqlahslg
       61 ggtgsgmgtl liskireeyp drimntfsvm pspkvsdtvv epynatlsvh qlventdety
      121 cidnealydi cfrtlklttp tygdlnhlvs vtmsgvitcl sfpgqlnadl rklavnmvpf
      181 prlhffmpgf apltsrgsqq yraltvpelt qqmfdaknmm aacdprhgry ltvatifrgr
      241 msmkevdeqm lnvqnknssy fvewipknvk tavcdipprg lkmsatfign staihelfnr
      301 iseqftamfr rkaflhwytg egmdemefte aesnmndlvs eyqqyqeata ddegefdded
      361 aladta
//
LOCUS       XP_077503449             322 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like [Amblyomma americanum].
ACCESSION   XP_077503449
VERSION     XP_077503449.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647323.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..322
                     /product="tubulin beta chain-like"
                     /calculated_mol_wt=35424
     Region          <13..307
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..322
                     /gene="LOC144113925"
                     /coded_by="XM_077647323.1:1..969"
                     /db_xref="GeneID:144113925"
ORIGIN      
        1 mqvayicavt fagqsgtgnn wakghytega elvdsvldvv rkeaescdcl qgfqlahslg
       61 ggtgsgmgtl liskireeyp drimntfsvm pspkvsdtvv epynatlsvh qlventdety
      121 cidnealydi cfrtlklttp tygdlnhlvs vtmsgvttcl sfpgqlnadl rklavnmvpf
      181 prlhffmpgf apltsrgsqq yraltvpelt qqmfdaknmm aacdprhgry ltvatifrgr
      241 msmkevdeqm lnvqnknssy fvewipnnvk tavcdipprg lkmsatfign staiqelfkr
      301 iseqftvdnp ngdkgpspsl ar
//
LOCUS       XP_077503463             396 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like [Amblyomma americanum].
ACCESSION   XP_077503463
VERSION     XP_077503463.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647337.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..396
                     /product="tubulin beta chain-like"
                     /calculated_mol_wt=44324
     Region          7..396
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..396
                     /gene="LOC144113931"
                     /coded_by="XM_077647337.1:1..1191"
                     /db_xref="GeneID:144113931"
ORIGIN      
        1 mtdeyrgkyv prailldlep gtmdsvrsgp fgqlfrpdnf ifgqsgagnn wakghytega
       61 elvdsvldvv rkeaescdcl qgfqlahslg ggtgsgmgtl liskireeyp drimntfsvm
      121 pspkvsdtvv epynatlsvh qlvensdety cidnealydi cfrtlklttp tygdlnhlvs
      181 vtmsgvttcl rfpgqlnadl rklavnmvpf prlhffmpgf apltsrgsqq yraltvpelt
      241 qqmfdaknmm aacdprhgry ltvatifrgr msmkevdeqm lnvqnknssy fvewipnnvk
      301 tavcdipprg lkmsatfign staiqelfkr iseqftamfr rkaflhwytg egmdemefte
      361 aesnmndlvs eyqqyqeata ddegefdned aladta
//
LOCUS       XP_077503494             396 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like [Amblyomma americanum].
ACCESSION   XP_077503494
VERSION     XP_077503494.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647368.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..396
                     /product="tubulin beta chain-like"
                     /calculated_mol_wt=44323
     Region          7..380
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..396
                     /gene="LOC144113950"
                     /coded_by="XM_077647368.1:1..1191"
                     /db_xref="GeneID:144113950"
ORIGIN      
        1 mtdeyrgkyv prailldlep gtmdsvrsgp fgqlfrpdnf ifgqsgagnn wakghytega
       61 elvdsvldvv rkeaescdcl qgfqlahslg ggtgsgmgtl liskireeyp drimntfsvm
      121 pspkvsdtvv epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs
      181 vtmsgvttcl rfpgqlnadl rklavnmvpf prlhffmpgf apltsrgsqq yraltvpelt
      241 qqmfdaknmm aasdprhgry ltvatifrgr msmkevdeqm lnvqnknssy fvewipnnvk
      301 tavcdipprg lkmsatfign staiqelfkr iseqftamfr rkaflhwytg egmdemefte
      361 aesnmndlvs eyqqyqeata ddegefdded aladta
//
LOCUS       XP_077503503             454 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4B chain-like [Amblyomma americanum].
ACCESSION   XP_077503503
VERSION     XP_077503503.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647377.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 22% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..454
                     /product="tubulin beta-4B chain-like"
                     /calculated_mol_wt=50989
     Region          <1..36
                     /region_name="Tubulin_FtsZ_Cetz-like"
                     /note="Tubulin protein family of FtsZ and CetZ-like;
                     cl10017"
                     /db_xref="CDD:471962"
     Region          65..438
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..454
                     /gene="LOC144113958"
                     /coded_by="XM_077647377.1:1..1365"
                     /db_xref="GeneID:144113958"
ORIGIN      
        1 mfrrkaflhw ytgegmdeme fteaesnmnd lvseyqhnea adslaresrs raqadlvgdf
       61 skergkyvpr ailldlepgt mdsvrsgpfg qlfrpdnfif gqsgagnnwa kghytegael
      121 vdsvqdvvrk eaescdclqg fqlahslggg tgsgmgtlli skireeypdr imntfsvmps
      181 pkvsdtvvep ynatlsvhql ventdetyci dnealydicf rtlklttpty gdlnhlvsvt
      241 msgvttclrf pgqlnadlrk lavnmvpfpr lhffmpgfap ltsrgsqqyr altvpeltqq
      301 mfdaknmmaa cdprhgrylt vatifrgrms mkevdeqmln vqnktssyfv ewipnnvkta
      361 vcdipprglk tsatfignst aiqelfkris eqftamfrrk aflhwytgeg mdemefteae
      421 snmndlvsey qqyqeatadd egefddedal adta
//
LOCUS       XP_077503514             303 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain [Amblyomma americanum].
ACCESSION   XP_077503514
VERSION     XP_077503514.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647388.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..303
                     /product="tubulin beta chain"
                     /calculated_mol_wt=33508
     Region          <13..303
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..303
                     /gene="LOC144113967"
                     /coded_by="XM_077647388.1:1..912"
                     /db_xref="GeneID:144113967"
ORIGIN      
        1 mqvayicavt fagqsgagnn wakghytega elvdsvldvv rkeaescdcl qgfqlahslg
       61 ggtgsgmgtl liskireeyp drimntfsvm pspkvsdtvv epynatlsvh qlventdety
      121 cidnealydi cfrtlklttp tygdlnhlvs vtmsgvttcl rfpgqlnadl rklavnmvpf
      181 prlhffmpgf apltsrgsqq yraltvpelt qqmfdaknmm aacdprhgry ltvatifrgr
      241 msmkevdeqm lnvqnknsny fvewipnnvk tavcdipprg lkmsatfign staiqelfkr
      301 ise
//
LOCUS       XP_077503537             322 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like [Amblyomma americanum].
ACCESSION   XP_077503537
VERSION     XP_077503537.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647411.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..322
                     /product="tubulin beta chain-like"
                     /calculated_mol_wt=35478
     Region          <13..307
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..322
                     /gene="LOC144113985"
                     /coded_by="XM_077647411.1:1..969"
                     /db_xref="GeneID:144113985"
ORIGIN      
        1 mqvayicavt fagqsgagnn wakghytega elvdsvldvv rkvaescdcl qgfqlahslg
       61 ggtgsgmgtl liskireeyp drimntfsvm pspkvsdtvv epynatlsvh qlventdety
      121 cidnealydi cfrtlklttp tygdlnhlvs vtmsgvttcl rfpgqlnadl rklavsmvpf
      181 prlhffmpgf apltsrgsqq yraltvpelt qqmfdaknmm aacdprhgry ltvatifrgr
      241 msmkevdeqm lnvqnknssy fvewipnnvk tavcdipprg lkmsatfign staiqelfkr
      301 iseqftvdnp nedkgpspsl ar
//
LOCUS       XP_077503556             449 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4B chain-like [Amblyomma americanum].
ACCESSION   XP_077503556
VERSION     XP_077503556.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647430.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 12% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..449
                     /product="tubulin beta-4B chain-like"
                     /calculated_mol_wt=49730
     Region          12..433
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..449
                     /gene="LOC144114003"
                     /coded_by="XM_077647430.1:1..1350"
                     /db_xref="GeneID:144114003"
ORIGIN      
        1 mvwgcvwvda tcdehgatav vrnphiaslt felpsgttpe gaektaiala ytnvgyiisg
       61 kyvprailld lepgtmdsvr sgpfgqlfrp dnfifgksga gnnwakghyt egaelvdsvl
      121 dvvrkeaesc dslqgfqlah slgggtgsgm gtlliskire eypdrimntf svmpspkvsv
      181 tvvepynatl svhqlventd etycidneal ydicfrtlkl ttptygdlnh lvsvtmsgvt
      241 tclrfpgqln adlrklavnm vpfprlhffm pgfapltsrg sqqyraltvp eltqqmfdak
      301 nmmaacdprh gryltvatif rgrmsmkevd eqmlnvqnkn ssyfvewipn nvktavcdip
      361 prglkmsatf ignstaiqel fkriseqfta mfrrkaflhw ytgegmdeme fteaesnmnd
      421 lvseyqqyqe ataddegefd dedaladta
//
LOCUS       XP_077503577             399 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077503577
VERSION     XP_077503577.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647451.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..399
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=43897
     Region          12..389
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..399
                     /gene="LOC144114013"
                     /coded_by="XM_077647451.1:1..1200"
                     /db_xref="GeneID:144114013"
ORIGIN      
        1 mvwgcvwvda tcdehgatav vrnphiaslt felpsgttpe gaektaiala ytnvgyiisg
       61 kyvprailld lepgtmdsvr sgpfgqlfrp dnfifgksga gnnwakghyt egaelvdsvl
      121 dvvrkeaesc dslqgfqlah slgggtgsgm gtlliskire eypdrimntf svmpspkvsv
      181 tvvepynatl svhqlventd etycidneal ydicfrtlkl ttptygdlnh lvsvtmsgvt
      241 tclrfpgqln adlrklavnm vpfprlhffm pgfapltsrg sqqyraltvp eltqqmfdak
      301 nmmaacdprh gryltvatif rgrmsmkevd eqmlnvqnkn ssyfvewipn nvktavcdip
      361 prglkmsatf ignstaiqel fkriseqfte lppfnpahl
//
LOCUS       XP_077503588             366 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like [Amblyomma americanum].
ACCESSION   XP_077503588
VERSION     XP_077503588.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647462.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..366
                     /product="tubulin beta chain-like"
                     /calculated_mol_wt=40814
     Region          <13..350
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..366
                     /gene="LOC144114023"
                     /coded_by="XM_077647462.1:1..1101"
                     /db_xref="GeneID:144114023"
ORIGIN      
        1 mpvayicavt fagqsgagnn wakghytega elvdsvldvv rkeaescdcl qgfqlahslg
       61 ggtgsgmgtl liskireeyp drimntfsvm pspkvsdtvv epynatlsvh qlventdety
      121 cidnealydi cfrtlklttp tygdlnhlvs vtmsgvttcl rfpgqlnadl rklavnmvpf
      181 prlhffmpgf apltsrgsqq yraltvpelt qqmfdaknmm aacdprhgry ltvatifrgr
      241 msmkevdeqm lnvqnknlsy fvewipnnvk tavcdipprg lkmsatfigk staiqelfkr
      301 iseqftamfr rkaflhwytg egmdemefte aesnmndlvs eyqqyqeata ddegefdded
      361 aladta
//
LOCUS       XP_077503622             385 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077503622
VERSION     XP_077503622.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647496.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 11% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..385
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=43264
     Region          7..374
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..385
                     /gene="LOC144114047"
                     /coded_by="XM_077647496.1:1..1158"
                     /db_xref="GeneID:144114047"
ORIGIN      
        1 mtdeyrgkyv prailldlep gtmdsvrsgp fgqlfrpdnf ifgqsgagnn wakghytega
       61 elvdsvldvv rkeaescdcl qgfqlahslg ggtgsgmgtl liskireeyp drimntfsvm
      121 pspkvsdtvv epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs
      181 vtmsgvttci rfpgqlnadl rklavnmvpf prlhffmpgf apltsrgsqq yraltvpelt
      241 qqmfdaknmm aacdprhgry ltvatifrgr msmkeveeqm lnvqnknssy fvewipnnvk
      301 tavcdipprg lkmsatfign staiqelfkr iseqftamfr rkaflhwytg egmdemefte
      361 aesnmndlvy eyqqiwapgq gnird
//
LOCUS       XP_077503633             330 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like [Amblyomma americanum].
ACCESSION   XP_077503633
VERSION     XP_077503633.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647507.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 12% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..330
                     /product="tubulin beta chain-like"
                     /calculated_mol_wt=36422
     Region          1..315
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..330
                     /gene="LOC144114052"
                     /coded_by="XM_077647507.1:1..993"
                     /db_xref="GeneID:144114052"
ORIGIN      
        1 mdsvrsgpfg qlfrpdnfif gqsgagnnwa kghytegael vdsvldvvrk eaescdclqg
       61 fqlahslggg tgsgmgtlli skirveypdr imntfsvmps pkvsdtvvep ynatlsvhql
      121 ventdetyci dnealydicf rtlklttpty gdlnhlvsvt msgvttclrf pgqlnadlrk
      181 lavnmvpfpr lhffmpgfap ltsrgsqqyr altvpeltqq mfdaknmmaa cdprhgrylt
      241 vatifrgrms mkevdeqmln vqnenssyfv ewipnnvkta vcdipprglk msatfignst
      301 aiqelfkris eqftvdnpng dkgaspslar
//
LOCUS       XP_077503652             366 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like [Amblyomma americanum].
ACCESSION   XP_077503652
VERSION     XP_077503652.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647526.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..366
                     /product="tubulin beta chain-like"
                     /calculated_mol_wt=40920
     Region          <13..350
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..366
                     /gene="LOC144114066"
                     /coded_by="XM_077647526.1:1..1101"
                     /db_xref="GeneID:144114066"
ORIGIN      
        1 mqvayicavt fagksgagnn wakghytega elvdsvldvv rkeaescdsl qgfqlahslg
       61 ggtgsgmgtl liskireeyp drimntfsvm pspkvsdtvv epynatlsvh qlventdety
      121 cidnealydi cfrtlklttp tygdlnhlvs vtmsgvttcl rfpgqlnadl rklavnmvpf
      181 prlhffmpgf apltsrgsqq yraltvrelt qqmfdaknmm aacdprhgry ltvatifrgr
      241 msmkevdeqm lnvqnknssy fvewipnnvk tavcdipprg lkmsatfign staiqelfkr
      301 iseqftamfr rkaflhwyte egmdemefte aesnmndlvs eyqqyqeata ddegefdded
      361 aladta
//
LOCUS       XP_077503663             398 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077503663
VERSION     XP_077503663.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647537.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 27% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..398
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=44209
     Region          1..356
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..398
                     /gene="LOC144114076"
                     /coded_by="XM_077647537.1:1..1197"
                     /db_xref="GeneID:144114076"
ORIGIN      
        1 mdsvrsgpfg qlfrpdnfmf gqsgagnnwa kghytegael vdsvldvvrk eaescdclqg
       61 fqlahslggg tgsgmgtlli skireeypdr imntfsvmps pkvsdtvvep ynatlsvdql
      121 ventdetyci dnealydicf rtlklttpty gdlnhlvsvt msgvttclrf pgqlnadlrk
      181 lavnmvpfpr lhffmpgfap ltsrgsqqyr altvpeltqq msdaknmmaa cdprhgrylt
      241 vatifrgrms mkevdeqmln vqnknssyfv ewipnnvkta vcdipprglk msatfignst
      301 aiqelfkris eqftamfrre aflhwytgeg mnemefteae snmndlvsey qhneaadsla
      361 resrsraqav latlahilps cpaspppagl vplrtwed
//
LOCUS       XP_077503679             376 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077503679
VERSION     XP_077503679.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647553.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..376
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=41962
     Region          7..338
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..376
                     /gene="LOC144114078"
                     /coded_by="XM_077647553.1:1..1131"
                     /db_xref="GeneID:144114078"
ORIGIN      
        1 mtdeyrgkyv prailldlep gtmdsvrsgp fgqlfrpdnf ifgqsgagnn wakghytega
       61 elvdsvldvv rkeaescdcl qgfqlahslg ggtgpgmgtl liskireeyp driintfsvm
      121 pspkvsdtvv epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs
      181 vtmsgvttcl rfpgqlnadl rklavnmvpf prlhffmpgf apltsrgsqq yraltvpelt
      241 qqmfdaknmm aacdprhgry ltvatifrgr msmkevdeqm lnvqkknssy fvewipnnvk
      301 tavcdipprg lkmsatfign staiqelfkr iseqftdmph vanpqfpvrw rssaphafsi
      361 rncvadslam lqftqr
//
LOCUS       XP_077503694             271 aa            linear   INV 25-NOV-2025
DEFINITION  methylthioribose-1-phosphate isomerase-like [Amblyomma americanum].
ACCESSION   XP_077503694
VERSION     XP_077503694.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647568.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 18% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..271
                     /product="methylthioribose-1-phosphate isomerase-like"
                     /calculated_mol_wt=29296
     Region          3..>185
                     /region_name="PRK08535"
                     /note="ribose 1,5-bisphosphate isomerase; cl00348"
                     /db_xref="CDD:444854"
     Region          196..>236
                     /region_name="PTZ00010"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:240228"
     CDS             1..271
                     /gene="LOC144114081"
                     /coded_by="XM_077647568.1:140..955"
                     /db_xref="GeneID:144114081"
ORIGIN      
        1 mlqsirykag tleildqrrl pheihyltla dakdvseair fmkvqgapai amvaalgvav
       61 dltqrarlpp sradlaalvh flldyistsr praanlayva ahlgsmadtl akyeglsvea
      121 mrdiicaeae gmlaadfaan qligqygadh vvancraaag ddklgvlthc nagtlatagy
      181 gtalgeeaka llleefwevi sdehgidptg syhgdsdlql erinvyynea tdmphvatpq
      241 fpvrwrssap hafsirncva dllamfqftq r
//
LOCUS       XP_077503706             842 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114088 [Amblyomma americanum].
ACCESSION   XP_077503706
VERSION     XP_077503706.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647580.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 12% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..842
                     /product="uncharacterized protein LOC144114088"
                     /calculated_mol_wt=94920
     Region          198..287
                     /region_name="SCAN"
                     /note="SCAN domain; pfam02023"
                     /db_xref="CDD:460417"
     Region          355..370
                     /region_name="zf-CCHC"
                     /note="Zinc knuckle; pfam00098"
                     /db_xref="CDD:395050"
     Region          637..693
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          713..805
                     /region_name="rve"
                     /note="Integrase core domain; pfam00665"
                     /db_xref="CDD:459897"
     CDS             1..842
                     /gene="LOC144114088"
                     /coded_by="XM_077647580.1:1027..3555"
                     /db_xref="GeneID:144114088"
ORIGIN      
        1 mdlmkllrsd llslceelgv dveekmkksh ictllletan eetvsdtwel lkaerkkked
       61 erkreeterk keenerqrla aereerelrr lqlandqkkl egessrgmtp ervsqaesfp
      121 mdrlmqpyki gedlgyylvn fertcekrgy aketwpqkll mvlpceaati varldakdad
      181 dyekvkasll rkyrlsaeef rqrfrgaskr ssesyaefaf slkanltewl kgaeaydtkd
      241 kvvecvgleq fynsipeavk lwvqdrenvs tvekaaelad eyatrrklss essesekkaf
      301 glrkrfipkn rsqfrnretv egtkqapekt edrakpagaq kaltkefear kpfrcfncqe
      361 tghiaarctk trlafsyand sdenlellrp yihelqvngk lcrvlrdsga tmdivhpsyv
      421 keedftgeva wirqvlenqs vclpmarvvi sgpfgelvte aavssavslq ypylfsnksn
      481 qllcdrgqkl aegtvkaltr sksrelaskl rysqeseatg eregipeiae pskenavrah
      541 eqqqepvtec spidaslqea cspdsmllpt slcmdrllrv drdsliteqe rdpslsklhf
      601 iakegiarrn vtlhhkggll yrhyrdkkgk tldqlvvpek yrsdllglch gnswsghlgv
      661 nktkerllge yywpgcfrda eryvqscdac qrtgkpgerw kaplklvpli sepfrrlvid
      721 tvgplprtks gykyvltmic patkfpeavp lkelssaeiv dgllsvfari gfpaeiqadq
      781 gtvftsaltt tflqrcgvgp gpgraltrpk aepgraacgs vgpgpgagse lrpvhssssa
      841 ar
//
LOCUS       XP_077503735             454 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-4-like [Amblyomma americanum].
ACCESSION   XP_077503735
VERSION     XP_077503735.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647609.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 24% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..454
                     /product="protein argonaute-4-like"
                     /calculated_mol_wt=50053
     Region          <8..>78
                     /region_name="Gag_spuma"
                     /note="Spumavirus gag protein; pfam03276"
                     /db_xref="CDD:460872"
     Region          140..>449
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     CDS             1..454
                     /gene="LOC144114113"
                     /coded_by="XM_077647609.1:1..1365"
                     /db_xref="GeneID:144114113"
ORIGIN      
        1 mhngkkksds adgrpavpsv sggnqatvsp pgpsrvsfeq alqqgfaasp qpravsgsqt
       61 paaersaqps vepsgyrpiy gaaaaagaan vaasspwpas aaaavsgcqp aaaapppspp
      121 ctppiavteq tpeagdkttp gddvkirele rtlpsnfprr pahgqlgrti qllanhfsie
      181 iptgsvyhyd vdissetaqe tkvpeqnkyg clstkinriv iellvkkyrq dlancipafd
      241 grknlytrrq vnfrertftv dleedqrsqk fiikiqyaat vnlealhdvf ekrvqtvpqe
      301 vlqainivlr hspsinlapv rrsffrppgp nehndlgggr evwfgyytsv rpaqwkpmln
      361 vdmsatafye slplvdfmcr flsdsrrvlt padfrslrdn qyvrlnrelk glrvkvthlp
      421 hprkykvvni trepakdiyf esedgsdfel qqrt
//
LOCUS       XP_077503749             365 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114117 [Amblyomma americanum].
ACCESSION   XP_077503749
VERSION     XP_077503749.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647623.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 41% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..365
                     /product="uncharacterized protein LOC144114117"
                     /calculated_mol_wt=39384
     Region          207..>314
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     CDS             1..365
                     /gene="LOC144114117"
                     /coded_by="XM_077647623.1:1..1098"
                     /db_xref="GeneID:144114117"
ORIGIN      
        1 mcespciaaq qgqaatsssp sgapidepet lakrlrlktl skgdgcpvfc frwciyacrn
       61 drydrkrsry ikptsnghfr laavpsvsgg nqatasppgp srvsfeqalq qgvaaspppr
      121 avsgsqtpaa ersaqpsvep sgyrpsygaa aaagaanvaa sspwpasaaa avsgcqpaaa
      181 apppsppctp pvvvteqtpe agdqttpgdd vkirelertl psnfprrpah gqlgrtiqll
      241 anhfsieipt gsvyhydvdi ssetaqetkv peqnnkkyrq dlancipafd grknqytrrq
      301 vnvrertftv dleedqrsqk iiikiqyaat vnlealhglf ekrrrikwdt ksfsvspety
      361 apvkw
//
LOCUS       XP_077503760             388 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114124 [Amblyomma americanum].
ACCESSION   XP_077503760
VERSION     XP_077503760.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647634.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 42% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..388
                     /product="uncharacterized protein LOC144114124"
                     /calculated_mol_wt=41212
     Region          140..>310
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     CDS             1..388
                     /gene="LOC144114124"
                     /coded_by="XM_077647634.1:1..1167"
                     /db_xref="GeneID:144114124"
ORIGIN      
        1 mhngkkksds adgrpavpsa sggnqatasp agpsrvsfeq alqqgvaasp ppravsgsqt
       61 paaersaqps vepsgyrpsy gaaaaagaan vaasspwsas aaaavsgcqp aaaapppspp
      121 ctspvvvteq tpeagdqttp gddvkirelk rtlpshfprr pahgqlgrti qllanhfsie
      181 iptgsvyhyg vdissetaqe tkvpeqkkyr clstkinriv iellvkkyrq dlancipafd
      241 grknlytrll vnfrertftv nleedqrsqk fvikiqyaat vnletlhgvf gkrvqtvpqe
      301 vlqaidivlr ggdagtesqd vdprelmdvv igsigevsqp sspqsqddgg nsrrvpleps
      361 ptispedlss eiqqplysfg pfasfvlw
//
LOCUS       XP_077503770             854 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-4-like [Amblyomma americanum].
ACCESSION   XP_077503770
VERSION     XP_077503770.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647644.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..854
                     /product="protein argonaute-4-like"
                     /calculated_mol_wt=95833
     Region          157..838
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     CDS             1..854
                     /gene="LOC144114129"
                     /coded_by="XM_077647644.1:1..2565"
                     /db_xref="GeneID:144114129"
ORIGIN      
        1 mdepetlskr lrlktlsrar nkryikptsn ghfrlaavps vsggnqatas ppgpsrvsfe
       61 qalqqgvaas pqpravsgsq tpaaersaqp sgyrpsyraa aasgaanvaa sspwpasaaa
      121 avsgyqpaaa apppspprtp pvvvteqtpe agdqttpgdd vkirelertl pshfprrpah
      181 sklgrtiqll anhfsieipt givyhydvdi ssetaqetkv peqkkyrclc tkinriviel
      241 lvkkyrqdla ncipafdgrk nlytrrqvnf rertftvdle edqrsqkfii kiqyaatvnl
      301 ealhgvfekr vqtvpqevlq aidvvlrhsp sfnlapvgrs ffrppgpneh ndlgggrevw
      361 fgyytsvrpa qwkpmlnvdm satafyeslp lvdfmcrfls dsrrvltpad frslrdnqyv
      421 rlnrelrvkv thlphprkyk vvkitrepak diyfesegsq isvadnfqsr yrrlsypnfp
      481 cvrsgspthp vyiplevcql aegqhcrkkl desqtaemik rtgkppakrf heirqsvcdm
      541 vsssdkylre fgvkintept qvvgrvldpp slvfenntmc kprdgtwdlr gqrfymamsm
      601 tqwivlnvsr fahkdclenf kmlirigqel gmhiaqplav itfdtnrkam rtvlteqrkq
      661 hpqlemvvav itkatnyaei kqvaenelsl rtqcildnvv qkcnaaliqn lcqkinakmg
      721 ginnsllmqe kpkllhrpvi vigadvshps pgdkvrpsia acvgslgsvp skfhatirvq
      781 iedseakarv eiikdlkdmm kelllvfcrt tghkperivf yrdgvsegqf levrnhevsn
      841 cayyntaptk rssc
//
LOCUS       XP_077503782             569 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-4-like [Amblyomma americanum].
ACCESSION   XP_077503782
VERSION     XP_077503782.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647656.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..569
                     /product="protein argonaute-4-like"
                     /calculated_mol_wt=63092
     Region          138..>553
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     CDS             1..569
                     /gene="LOC144114139"
                     /coded_by="XM_077647656.1:1..1710"
                     /db_xref="GeneID:144114139"
ORIGIN      
        1 mhngkkksds adgrpavpsv sggnqatasp agpsrvsfeq alqqgvaasp qpravsdsqt
       61 paaersarps vepggyrpsh gaaagaanva asspwpasav aavsgcqpaa aapppsppct
      121 ppvavteqtp eagdkttpgd dvkirelert lpshfprrpa hsqlgrtiql lanhfsieip
      181 tgivyhydvd issetaqetk vpeqkkyrcl stkinrivie llvkkyrqdl ancipafdgr
      241 knlytrrqvn frertftvdl eedqrsqkfi ikiqyvatvn lealhgvfkk rvqtvppevl
      301 qaidivlrhs psinlapvgr sffrppgpke hndlgggrev wfgyytsvrp aqwkpmlnvd
      361 msatafyesl plvdfmcrfl sdsrrvltpa dfrslrdnqy vrlnrelkgl rvkvthlpyp
      421 rkykvvkitr epakdiyfes egsqisvady fqsryrrlsy pnfpcvqsgs pthplyiple
      481 vcelaegqhc rkkldesqta emikrtakpp akrfheirqs vrdmvsssdk ylrefgekin
      541 teptqvvgrv ldptgwassa tflparpss
//
LOCUS       XP_077503795            1961 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: putative ATP-dependent RNA helicase TDRD12
            [Amblyomma americanum].
ACCESSION   XP_077503795
VERSION     XP_077503795.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647669.1
KEYWORDS    RefSeq; corrected model; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio   :: 1% of CDS bases
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1961
                     /product="LOW QUALITY PROTEIN: putative ATP-dependent RNA
                     helicase TDRD12"
                     /calculated_mol_wt=218139
     Region          15..140
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          700..1067
                     /region_name="DEAD-like_helicase_N"
                     /note="N-terminal helicase domain of the DEAD-box helicase
                     superfamily; cl28899"
                     /db_xref="CDD:475120"
     Site            order(767,771..775,884)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:350670"
     Region          1205..1341
                     /region_name="Tudor_SF"
                     /note="Tudor domain superfamily; cl02573"
                     /db_xref="CDD:470623"
     Site            order(1270,1273,1275,1296,1299,1301)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:410449"
     Region          1457..1573
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          1793..1887
                     /region_name="alpha-crystallin-Hsps_p23-like"
                     /note="alpha-crystallin domain (ACD) found in
                     alpha-crystallin-type small heat shock proteins, and a
                     similar domain found in p23 (a cochaperone for Hsp90) and
                     in other p23-like proteins; cl00175"
                     /db_xref="CDD:469641"
     CDS             1..1961
                     /gene="LOC144114147"
                     /coded_by="XM_077647669.1:145..6030"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:144114147"
ORIGIN      
        1 meafrseegw ksrvnvvvlk vqspsfflir eapgpdmssy trdflrlead ikknmeklsm
       61 kpwdpllpei gsvvlvknpk drewyrglvn qllddtdgfk vkvtlvdyge tlfvdrkqlh
      121 glfhqrllev pfqcikfslp elrpvqwaig ketlttkyia sefwdtaave yvklavrrad
      181 saqvqvlgst ssggltgklf igcnkaticl neelvklnya fqdvedgeaf mlqeerrllr
      241 tgqseqplvk eprleysghq iysgiqqsva aaafqnvyed ilerapgvhc ipvkvsqaea
      301 kvsppssppa ssassptqgs ssstsisiga astgsdtppt lpglgrglal mlrfdecrrp
      361 splqesrveg stndlaqesr spvlglrqdv asytptavat psknlssset agitdstnsk
      421 tstpvtqqtp vskdrpaltt skpcvpstrr nrkpkprgra rmaqpylack aqydpsvkvr
      481 pprtansdss kkvqeagtka enseasvqsk tsefrasdtg spvktdssly rsagqgharl
      541 qfkptschvf gqqvsslple pmnaqqkdsv elcegpesgs sqckplptcv dfsdtspcpv
      601 skaqrqkdll qqlesalnga plrstcsles qavedaspav qkpewhleft fhqapmnvsq
      661 gledlqfhdl qsssefqpds dpseyfsgpv dfitnydsfn ltesgyskvr vlchglrvpi
      721 aqedldktii ykplkenlrs qgiraptsiq svvwpavdsg rtivavgsph sgktlaylip
      781 lvsrvltetd yerlpegpgp lvlilastwk gvcriyeqfk lltekidgpr ctvlyaggse
      841 kgkevevvng cdilvatpnt flrylenfnr lvvnlyrcch lvlddgerll dkyasevakv
      901 vnelwksqac rhpsirleqi ivcstvwsrc vdlflhkllv nksplvffss ffeaaiyakv
      961 qtvthyaepd mhrdtllgil rgsqgqkvvv ctstreaava lnrllslenv ysllihdrlp
     1021 makisevqre wmgeyariqt pilvvqdsvl wlakirdatv lvhydiptss ktsfgfrysc
     1081 lasymqsfra kdnislpsgg pvahmivssn ernasvrlve fltkcgssvp gelmelaaqe
     1141 eargssnaev alcpnlkafg hceqpageks csyrhrilpa adhspvwsdl psegnvqivv
     1201 trvvsascfy awilqqwdtp sgsttvtnle velqeamlsl nehlshsenc arlkeeavpi
     1261 igqvyalevs vkrfervlvt slvpgelapp yatvshidyg gestvatvrl ielpqrlaql
     1321 rpfavevycc riqpqdrdis wtyqadllty nhysrkelfg kvvlrlgntl wldplvmrer
     1381 lpsvnvevtf gnvrstlite glactnenhm ktlhkmvvda gitvpplppg kkerneidsa
     1441 daatvsrhcs sylnstdfch vylwtvvsps qfyvqtlefn scleqlendi qraverqqvg
     1501 klkavhigvc clarysndrw yrgevlkihd dkavdiffpd ygdtetysrd qllepaswmm
     1561 llpyqgiscs lagigpqtee wsptaqslle dfgyndedmn rvlclrvask ragthpgnnh
     1621 yevflfdgch dgrisigdll vekgiavrtk lpelefdvdr phvslfeqnv agpsndsddd
     1681 edddpaisqc rknleehvyk vfsdvrdqvl apataeimee malstgnlec gdtacssqvq
     1741 gvggpstqfn qdarkktase nsmgkkmsqe cslkdiptlg sifktpsshq akiswwqddr
     1801 fvyvdilvak aqnynfwvst tsllcrvfta eheylvhepl faailpekvr lvkkaeclki
     1861 tlekaqskiy wdfltghrrk ipyihydleh ivvsdddddi vsslqaykdp dftgyerpev
     1921 ilpydpaahv drlleiegde dehsdssddm ycskdpndif g
//
LOCUS       XP_077503827             584 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114149 [Amblyomma americanum].
ACCESSION   XP_077503827
VERSION     XP_077503827.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647701.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 48% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..584
                     /product="uncharacterized protein LOC144114149"
                     /calculated_mol_wt=63772
     Region          <365..413
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     CDS             1..584
                     /gene="LOC144114149"
                     /coded_by="XM_077647701.1:1..1755"
                     /db_xref="GeneID:144114149"
ORIGIN      
        1 mstqspgrgs spwsaslspd llpletflrs gvgsipvvlv pqdggaikmt npgavraalq
       61 satshyesis evrqfgrggi lcrspdldcv rellkassfa slqvssfips hlactkgivr
      121 gvdsslspae tlellsaagv vavhrcsrdv nnmrvptesm iatfvgtscp seikawhlif
      181 rvdtlssrpv qcnncwrygh saggcksnlr ccncgdghat stcteqnekw clcggahsan
      241 ysncpsraqe iqileiidrk rcsrrdaitq lkerahgyag vtarqgvmld smlsqaiaaa
      301 veasmskmae rivasvsecl tnvlatqlth avqagverkl ldaqlgftls yisggmkdrw
      361 ilpglvfadd vvlmaethae lqalinvcae amaplglnfn akinlldith keclergqra
      421 vgrlapwchg rvtneavqgv lgwstfearg arskiaydgr lrlmhkdrwa rrvfycvsqn
      481 clritwverl qyvrrkfgff snevqagsar kwakaveaqv raqereawra ategkstlev
      541 hraakqdicv eslydnsvgs rplfearaga mptltyrkrf dttv
//
LOCUS       XP_077503852             507 aa            linear   INV 25-NOV-2025
DEFINITION  protein unc-93 homolog A-like [Amblyomma americanum].
ACCESSION   XP_077503852
VERSION     XP_077503852.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647726.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..507
                     /product="protein unc-93 homolog A-like"
                     /calculated_mol_wt=56797
     Region          91..497
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(93..94,97,125,175..176,178..180,183,218,221..222,
                     225,331,334..335,338..340,343,370,374,419..420,424,428,
                     451,454..455,458..459,462)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:349949"
     CDS             1..507
                     /gene="LOC144114170"
                     /coded_by="XM_077647726.1:102..1625"
                     /db_xref="GeneID:144114170"
ORIGIN      
        1 meqsqleqqe eqqhehkqeq qleqertqer aqerahtreh rrdhrhsskt rashsnvraa
       61 eedmrpspse akkqivrnll vvslsflllf tayqsmqnlq sslnpeaglg taslasiyfa
      121 lvvscmfvps ymirrlglky tmvtsvtmym lyfvanmeps wytmlpaslv lgtggaplwt
      181 skcaylayla qeyadlagal dsapfvagff giffmlfqtt qiwgnliafi vlrppnvtav
      241 aadielcgas fhptqdlavl nqtnlgavnr vqrytlsgvy tccvlssivl lvaflgpvrk
      301 evpdpnppmq llvetmnhmr hgyqqliipl tiysgmqqaf fsgdftqayi scawgvqfvg
      361 fvmisyglad accsmtfgfl vkrvgrvpiv llaallnagv milmltwrpr pseaynffvi
      421 allwgaadas wqtqinafyg iifseareaa fanyrlwesm gfivmfaiqs qlrvyvkvav
      481 lmvwlalgil gylviefhvs sdtrsll
//
LOCUS       XP_077503879             444 aa            linear   INV 25-NOV-2025
DEFINITION  protein unc-93 homolog A-like [Amblyomma americanum].
ACCESSION   XP_077503879
VERSION     XP_077503879.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647753.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 25% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..444
                     /product="protein unc-93 homolog A-like"
                     /calculated_mol_wt=49182
     Region          28..434
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(30..31,34,62,112..113,115..117,120,155,158..159,162,
                     268,271..272,275..277,280,307,311,356..357,361,365,388,
                     391..392,395..396,399)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:349949"
     CDS             1..444
                     /gene="LOC144114178"
                     /coded_by="XM_077647753.1:1..1335"
                     /db_xref="GeneID:144114178"
ORIGIN      
        1 mwpspseakk qvvrnllvvs lsflllftay qsmqnlqssl npeaglgtas lasiyfalvv
       61 scmfvpsymi rrlglkytmv tsvtmymlyf vanmepswyt mlpaslllgt ggaplwtskc
      121 aylaylaqey adlagaldsa pfvagffgif fmlfqttqiw gnliafivlr ppnvtavaad
      181 ielcgasfhp tqdlavlnqt nlgavnrvqr ytlsgvytcc vlssivllva fldpvrkevp
      241 dpnppmqllv etmnhmrhgy qqliipltiy sgmqqaffsg dftqayisca wgvqfvgfvm
      301 isygltdacc smtfgflvkr vgrvpivlla allnagvmil mltwrprpse aynffviall
      361 wgaadaswqt qinafygiif seareaafan yrlwesmgfi vmfaiqsqlr vyvkvavlmv
      421 wlalgilgyl viefhvssdt rsll
//
LOCUS       XP_077503892             475 aa            linear   INV 25-NOV-2025
DEFINITION  protein unc-93 homolog A-like [Amblyomma americanum].
ACCESSION   XP_077503892
VERSION     XP_077503892.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647766.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 12% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..475
                     /product="protein unc-93 homolog A-like"
                     /calculated_mol_wt=52589
     Region          28..434
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(30..31,34,62,112..113,115..117,120,155,158..159,162,
                     268,271..272,275..277,280,307,311,356..357,361,365,388,
                     391..392,395..396,399)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:349949"
     CDS             1..475
                     /gene="LOC144114185"
                     /coded_by="XM_077647766.1:1..1428"
                     /db_xref="GeneID:144114185"
ORIGIN      
        1 mwpspseakk qvvrnllvvs lsflllftay qsmqnlqssh npeaglgtas lasinfalvv
       61 scmfvpsymi rrlglkysmv tsvtmymlyf vanmepswyt mlpaslvlgt ggaplwtskc
      121 aylaylaqey adlagaldsa pfvagffgif fmlfqttqiw gnliafvvlr ppnvtavaad
      181 ielcgasfhp tqdlavlnqt nlgavnrvqr ytlsgvytcc vlssivllva fldpvrkevp
      241 dpnppmqllv etmnhmrhgy qqliipltiy sgmqqaffsg dftqayisca wgvqfvgfvm
      301 isygltdacc smtfgflvkr vgrvpivlla allnagvmil mltwhprpse aynffviall
      361 wgaadaswqt qiktfygiif seareaafan yrlwesmgfi vmfaiqsqlr vyvkvavlmv
      421 wlalgilgyl viefhmkdkh rrritnsata lnnycsiftt dngelplins sagsq
//
LOCUS       XP_077503944             125 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114218 [Amblyomma americanum].
ACCESSION   XP_077503944
VERSION     XP_077503944.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647818.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..125
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..125
                     /product="uncharacterized protein LOC144114218"
                     /calculated_mol_wt=13676
     CDS             1..125
                     /gene="LOC144114218"
                     /coded_by="XM_077647818.1:1..378"
                     /db_xref="GeneID:144114218"
ORIGIN      
        1 mgsvrffvvl sllslvvawt qaacpsirgq fsdcrfeqct keqcqrrgle ccpkpcggtw
       61 cvegvpnslm llaqythrcp qveppfpgqc evksqitced lncheqgaic cklacgeplc
      121 vpfte
//
LOCUS       XP_077503952             371 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114227 [Amblyomma americanum].
ACCESSION   XP_077503952
VERSION     XP_077503952.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647826.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..371
                     /product="uncharacterized protein LOC144114227"
                     /calculated_mol_wt=38792
     Region          30..113
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     CDS             1..371
                     /gene="LOC144114227"
                     /coded_by="XM_077647826.1:1..1116"
                     /db_xref="GeneID:144114227"
ORIGIN      
        1 mgsqqfclkw nnhqsnmlav fyqmlsneal vdvtlacegl slkahkmvls acspffqalf
       61 aenpckhpiv ilkdvryvdl raivefmyrg evvvpqedlm allktaetlk vkglvtsvdg
      121 aadsgpvgsp raespplskr krsrrprrrs psdsnksdse dappatrikv pdspeiiedg
      181 smssehmtdp htasppsras nvasstavps garhgssvqa sisqhtqdsi gddvgegdda
      241 dfdvepldvm eesattesvp vfpdvasssq algdesqqsh htgstsgnsa fvpaqpslps
      301 dipiksqvdi epsptsltpy ddkiiapvva aspaaassgd gastsmaatd asgvpaiagp
      361 sgyqqstpsh g
//
LOCUS       XP_077503973             113 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114242 [Amblyomma americanum].
ACCESSION   XP_077503973
VERSION     XP_077503973.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647847.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..113
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..113
                     /product="uncharacterized protein LOC144114242"
                     /calculated_mol_wt=12883
     CDS             1..113
                     /gene="LOC144114242"
                     /coded_by="XM_077647847.1:81..422"
                     /db_xref="GeneID:144114242"
ORIGIN      
        1 msvhyklcwh eeivykfkle wppdsadilh heqgiiesiv qmsregqcps slstaefavn
       61 iqlsewklsg liffekdgvt yepyhhqfll riqkkhvdlp ptaqmtpsvs ssg
//
LOCUS       XP_077503998             216 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114260 [Amblyomma americanum].
ACCESSION   XP_077503998
VERSION     XP_077503998.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647872.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..216
                     /product="uncharacterized protein LOC144114260"
                     /calculated_mol_wt=24161
     Region          <50..>216
                     /region_name="YkfC"
                     /note="Retron-type reverse transcriptase [Mobilome:
                     prophages, transposons]; COG3344"
                     /db_xref="CDD:442573"
     Region          <138..216
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            144
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238827"
     CDS             1..216
                     /gene="LOC144114260"
                     /coded_by="XM_077647872.1:1..651"
                     /db_xref="GeneID:144114260"
ORIGIN      
        1 mscdrgirks trtreipyvv rgqaygndgq ekteciedqe llsttmfclr arlsahdvll
       61 qlnrevigpl ygsqrdraif alylkgtfdn vkrsktqenl nttncgkrty ryvkdflmdr
      121 kayirtydsk lgpiemgtrg tpqgavmspl lfyiammdlp grlnaingvr hgiyadditl
      181 wsssgsigqi edalqeaasa vhkyaeecgl rcaptk
//
LOCUS       XP_077504021             197 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114272 [Amblyomma americanum].
ACCESSION   XP_077504021
VERSION     XP_077504021.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647895.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..197
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..197
                     /product="uncharacterized protein LOC144114272"
                     /calculated_mol_wt=22161
     CDS             1..197
                     /gene="LOC144114272"
                     /coded_by="XM_077647895.1:135..728"
                     /db_xref="GeneID:144114272"
ORIGIN      
        1 mkelsessti tfegrklqdq ynitvtwddi nskkqslpai iyiagfcaha alnklpceac
       61 arnltredrd ippdencliq gltrgalkfp qpvvvnavlh teivlekltt kenaaqfhst
      121 rhqreqllcl lkhllsddee ldvccnghhp dkvisnilwa stntymknyv qmktdlltak
      181 kaaaqerklk kfrclgg
//
LOCUS       XP_077504031             674 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114279 [Amblyomma americanum].
ACCESSION   XP_077504031
VERSION     XP_077504031.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647905.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..674
                     /product="uncharacterized protein LOC144114279"
                     /calculated_mol_wt=76646
     Region          4..93
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          275..459
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..674
                     /gene="LOC144114279"
                     /coded_by="XM_077647905.1:53..2077"
                     /db_xref="GeneID:144114279"
ORIGIN      
        1 mgrccvpncr gnydggpkvr vfsfpkddrr ikwkqairrd dldidtlrdp kvcelhfkpe
       61 ylrsttsytd adgktievam nltrltpdav ptilpncasy lsnsapiree pdakrrrrea
      121 trlqdaiems latheeeerr nkvssygqll slleglhvsd fwvmrktdaa vaffhvnens
      181 appeiersvv vnnslkvsaf wrkvelrgge mtvpdrledm rslesvlksv qqfkapdrce
      241 kdeklkacfq llfsllddls sddllpvekm etlrflkeql hllnrertaf rysselvils
      301 sifftispha ykfvrisgkl ilphpctitr lcnkynvspa aeqsdqgfls yitkkanllk
      361 phertvtlmm deihiqqyfe ykggsltgta snsseaakta hvfmtqslls ghkdvahilp
      421 vfsieasdlh evlkkvilgl ekaglkviav vsdnnsinrk vmslfskkka idivyphpad
      481 narplffavd pvhllkcirn nwlnqknpdt sflypefkst eskldikkas fqtlrdlyts
      541 erhnlskfay gltykalhpt nierqnvkla lkvinpfvaq alktpgtkpg fpyaadtaef
      601 idlihrwwci vntktpskgr hlrdpyqepv rnmtgvqlqf lgefvdwldt wndlkmsgsr
      661 sgsltpktha alra
//
LOCUS       XP_077504045             482 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077504045
VERSION     XP_077504045.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647919.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 41% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..482
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=54333
     Region          165..482
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..482
                     /gene="LOC144114286"
                     /coded_by="XM_077647919.1:1..1449"
                     /db_xref="GeneID:144114286"
ORIGIN      
        1 maaaraatnr ippsrkstaa plqaptfaie tssggsesar rrrsnhefva wlpvamvvgl
       61 fawayyvyif vfcgslvkeg aqrlafsivf hlllllclws fvqttvtavp pipgyfglse
      121 sdqrlleqya ddeargefld ilaenrgvlt rgpsggvrfc ercqqfwevi sdehgidpsr
      181 ayygdsdlql erinvyynea pggkyvprai lvdlepgtmd avrsgrfgql frpdnfvfvt
      241 fissvrrgpk thathnsfrt lklttptygd lnhlvsatms rvttclrfpg qlnadlrkla
      301 vnmvpfprlh ffmtgfaplt srdgqqyral tvpeltqqmf daknmmaacd prhgryltva
      361 avfrgrmsmr evddqmvniq nkdssyfvew ipnnvktavc dipprglkms atfignstai
      421 qelfkriseq ftamfrrkaf lhwytgegmd elefteaesn lnelvseyeq yqeataedeg
      481 ef
//
LOCUS       XP_077504056             757 aa            linear   INV 25-NOV-2025
DEFINITION  myosin phosphatase targeting subunit 75D [Amblyomma americanum].
ACCESSION   XP_077504056
VERSION     XP_077504056.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647930.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 30% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..757
                     /product="myosin phosphatase targeting subunit 75D"
                     /calculated_mol_wt=82168
     Region          <252..497
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          268..299
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(301,303,307..308,311..313,315..316,320,323,394,396,
                     398,402..403,406..408,410..411,415,418,427,429,431,
                     435..436,439..441,443..444,448,451)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          301..394
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          396..427
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          429..459
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <474..600
                     /region_name="metallo-dependent_hydrolases"
                     /note="Superfamily of metallo-dependent hydrolases (also
                     called amidohydrolase superfamily) is a large group of
                     proteins that show conservation in their 3-dimensional
                     fold (TIM barrel) and in details of their active site. The
                     vast majority of the members have a...; cl00281"
                     /db_xref="CDD:469705"
     CDS             1..757
                     /gene="MYPT-75D"
                     /coded_by="XM_077647930.1:1..2274"
                     /db_xref="GeneID:144114294"
ORIGIN      
        1 mseaerarek ergdaiirel krqlavaeek lrqereriee eersrraarv lpktarakpy
       61 sisandavet lvevgnnedh cggiqsapie nrqqesqvla ndaavvsaee prvvestknv
      121 dtdqqsfipk veasstdmsv qnsnsdqtlq vecceaaeqv iggangtder ndmncsvgpi
      181 apndlllfte apacqaekce sgmcvqpaca altaaskkpa pqmapslaai fcgvllsrrh
      241 vvcpevsalr fvrrllmlgv spdstnedgl talhqccidd seemmkllid fdanvnakds
      301 eqwtplhaaa tcghihlvry lisrgadlla vnadgnmpyd icedeptldy iesemakrgi
      361 tqemidetra anevmmlndl kslsetgsdl efrdeqgatp lhiaaangyv svveflldqh
      421 vstdvcdndq wqpihaaacw ghpdvlemlv qagadlnart kngetpydic edpdlkeriv
      481 qlrsemetkr amqpnklrrs qsqntrsqsv rrtsirekgm isrreareea rirqeqqapl
      541 gkdddeeavr rksegdvmng dsgsltspgs dgerpastvp svplslapat vavevagsss
      601 psrcglegvp epgslgslap mpppsysevs pmparhqgga aeavkveihv tvntqqasvg
      661 fspqngtlsd lkkhradlrn srgsltlsas qqqeivgkma asatalgqng hqhhfsatvr
      721 kpsyeappsp stslrrfrsd pseivgephr kdcctvm
//
LOCUS       XP_077504067             117 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114301 [Amblyomma americanum].
ACCESSION   XP_077504067
VERSION     XP_077504067.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647941.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..117
                     /product="uncharacterized protein LOC144114301"
                     /calculated_mol_wt=12725
     CDS             1..117
                     /gene="LOC144114301"
                     /coded_by="XM_077647941.1:1..354"
                     /db_xref="GeneID:144114301"
ORIGIN      
        1 mdmrcftegh lssllqclef kilcrnarvl lpwwntertr sswrtpdmtp epqsaaqhly
       61 vgdaflaani iswaitsvsd lpamitrptf gmfgltlsll cldggpsatc svaaagf
//
LOCUS       XP_077504079             164 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114303 [Amblyomma americanum].
ACCESSION   XP_077504079
VERSION     XP_077504079.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647953.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 16% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..164
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..164
                     /product="uncharacterized protein LOC144114303"
                     /calculated_mol_wt=18149
     CDS             1..164
                     /gene="LOC144114303"
                     /coded_by="XM_077647953.1:1..495"
                     /db_xref="GeneID:144114303"
ORIGIN      
        1 mdmgcfaegr lssllqclef kilcrnarvl lpwwnaeqts sswripdmtp epqsaaragl
       61 lvgrhrvgdv mklwvmrtet rlfsgrerna ttfrypvehc pfdkhlclgd aflaaniisw
      121 ritsdcdlpa mitrptfgmf gltlsllcld ggpspicsva atgl
//
LOCUS       XP_077504090             164 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114310 [Amblyomma americanum].
ACCESSION   XP_077504090
VERSION     XP_077504090.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647964.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 24% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..164
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..164
                     /product="uncharacterized protein LOC144114310"
                     /calculated_mol_wt=18161
     CDS             1..164
                     /gene="LOC144114310"
                     /coded_by="XM_077647964.1:1..495"
                     /db_xref="GeneID:144114310"
ORIGIN      
        1 mdmgcfaegh lssllqclef kilcrnarvl lpwwnaerts iswripdmtp epesaaqagl
       61 lvgrhrvgdv mklwvmrtet rlfsgrerna ttfrypvehc pfdkhlclgd aflaanifsw
      121 pitsdcdlpa mitlptygmf gftlsllcld ggpsptcyva atga
//
LOCUS       XP_077504100            1566 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: maestro heat like repeat family protein c11.1
            [Amblyomma americanum].
ACCESSION   XP_077504100
VERSION     XP_077504100.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647974.1
KEYWORDS    RefSeq; corrected model; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio   :: 1% of CDS bases
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1566
                     /product="LOW QUALITY PROTEIN: maestro heat like repeat
                     family protein c11.1"
                     /calculated_mol_wt=171309
     Region          <703..795
                     /region_name="PRK15111"
                     /note="peptide ABC transporter permease SapC"
                     /db_xref="CDD:185066"
     Region          1294..1315
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(1307..1308,1311,1314..1315,1351..1352,1355,1358,
                     1362,1365,1393..1394,1397,1400..1401,1404,1438..1439,1442,
                     1445..1446)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          1331..1365
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          1375..1405
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          1421..1446
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..1566
                     /gene="c11.1"
                     /coded_by="XM_077647974.1:226..4926"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 1 base in 1 codon"
                     /db_xref="GeneID:144114317"
ORIGIN      
        1 matdtafktg gqvdgivaal veslgdkqds vcravthslq vignrwpnlv lscchdyvst
       61 esklsashrs llfsvmhevc kesvdkldkt llsnvcktti eemtrskeva tdnvlcgivi
      121 algrqhphet melvqakfqp gsmphplvle tlaglalanp fgimpflkal lgtmlpmlsg
      181 arseqlkwam ayalgrfsea iveylsniek apdpsvqkas fateivsafd fllanwllar
      241 eakvraesav tlgymaqlls kpkleehlpk lllslvvlhr kqhdcfsvsk glclvleaat
      301 ehkcqaleqh ldainnslfv pvcsppdyte pasvrnynes lrcftviatm fpdkltafll
      361 mkvengnekm rigaltvlkh iinsagtseq dpnldermrn iigglqssfi epsnkakkvl
      421 aqvivalahh gyldangghs mieflvrqca lpddtakrpt dfdyvsneal rtmcenvlql
      481 ltttvddidk lmwpflfeyl lceeltpavg iickclanfa skkreagypi einyekhpnv
      541 pkpqxllarl lvllgsplmh hhrgvhvlrl lrclptlvhr nlvalwdtic pddssaaeks
      601 glvgcgffsv gcalavgmca ashldavvvk leqvakddlr knsgflsflk ditgdnyhek
      661 llatvvlsyg hvslrapasi lttrietpil rsaakfyqas kdpsvrhaml rtvkmiaeav
      721 hpdrlqeqyv fysrgelvae mqlllkaenp vlvtsttral vlmalaslvk ldpplppsev
      781 salvtmavsr vyplpvrsps vslddgaagd qphnhtelvs sclsaldqfl aellskdrtp
      841 dnlhliinvl vpwltskqef ererclnsiw elmmlysshi etgvfrifsc fgfilgalvp
      901 rctdssisir ntaltcihls leinnksqsl sskedtdmhg vddlqaciqk sdsesfhvis
      961 qelaktlakk vpaiqllsla ntllqglkdp vtssscgaas vlsgllqlrg aeirsevvhl
     1021 idslhqqlsh vhcpqthsea levvrtlarh hsslvvhkll iyplpydkhv cecwsalakd
     1081 asmgasivnq llemldnlga telqpdpksq rgtirvaard vlaaicalqe ifntpemeac
     1141 akeafgqvfs allltaasyv gvssvspfra ngssskqdsg agiklfplrc alqafeaflk
     1201 ragqqeivas mekdghwdsm eqdevfpdsv tvlagllcqq cpwqvagvvs glqnrlgsnp
     1261 leaqrlavla lfaellsqpf egapllaepl mshllgslad pslavrrlcl rslahlgrse
     1321 ghlvhrysgt vlsamisgld dkddteqkim leainglttl lshadpqdvg sfittvalrl
     1381 rpmfekekge vraaafklfg dlarfaegef rdafveqais nvitllvhln esdkhvvkac
     1441 katlkklgpl lgseelntmf qrhlpdgshl hysefisdla kvmvidleeh mstfvtlclg
     1501 hikslnpaiq cnavlllgcl lgnarpesrk dlpceracsa lilllkgelp eqrcraaeal
     1561 sllhsc
//
LOCUS       XP_077504107             248 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114329 [Amblyomma americanum].
ACCESSION   XP_077504107
VERSION     XP_077504107.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647981.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 32% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..248
                     /product="uncharacterized protein LOC144114329"
                     /calculated_mol_wt=28139
     CDS             1..248
                     /gene="LOC144114329"
                     /coded_by="XM_077647981.1:13..759"
                     /db_xref="GeneID:144114329"
ORIGIN      
        1 mvcmlkflll saalvsnqms sscveilrgy mantnranwq lvaqfvaada ivqlqrrrrr
       61 kpirrllrdr rqlgeyftlv rdmrngdgtd ffqyfrmsve rfdrlvellr plieprrstv
      121 vslsagekla ftlrylapgs tmriiaksyr isdsmagriv rqtclalrqv lepfiavdts
      181 tgtdllplle vqticgippp eevaridarl ptassperem rsararngvv hincplftmr
      241 cqshhqgv
//
LOCUS       XP_077504119             247 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase RNF212B-like [Amblyomma americanum].
ACCESSION   XP_077504119
VERSION     XP_077504119.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647993.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..247
                     /product="E3 ubiquitin-protein ligase RNF212B-like"
                     /calculated_mol_wt=27318
     Region          5..45
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(7,10,25,27,30,33,42,45)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438222"
     Region          92..>131
                     /region_name="PRK03992"
                     /note="proteasome-activating nucleotidase; Provisional"
                     /db_xref="CDD:179699"
     Region          <116..139
                     /region_name="ZIP_TSC22D-like"
                     /note="leucine zipper found in the TSC22 domain leucine
                     zipper transcription factors, c-Myc-binding protein, and
                     similar proteins; cl40461"
                     /db_xref="CDD:424092"
     CDS             1..247
                     /gene="LOC144114338"
                     /coded_by="XM_077647993.1:273..1016"
                     /db_xref="GeneID:144114338"
ORIGIN      
        1 mnklvycnnc faqptgtslf fvtncahvfc rgckeectrs kckicnatcs taplsqnmsa
       61 dvrelfkdpk iimqrammic gfqvsqlsgl saylrqqvns lkaslkaatd nwikhkqene
      121 rlrkevaalr sevkelkdqq spgieigqsp tfltrpaypi ppafeqptaa ppvarmedfg
      181 qrtpsmqnfs ggtigytpsr ratammastd lsqqrghrty ahddqpasrl dvaqatlafg
      241 ssprerr
//
LOCUS       XP_077504141             815 aa            linear   INV 25-NOV-2025
DEFINITION  piwi-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077504141
VERSION     XP_077504141.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648015.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..815
                     /product="piwi-like protein 1"
                     /calculated_mol_wt=93307
     Region          233..365
                     /region_name="PAZ"
                     /note="This domain is named PAZ after the proteins Piwi
                     Argonaut and Zwille; smart00949"
                     /db_xref="CDD:198017"
     Site            order(274,287,294,298,316,331,333)
                     /site_type="other"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]"
                     /db_xref="CDD:239211"
     Region          353..798
                     /region_name="Piwi_piwi-like_Euk"
                     /note="PIWI domain, Piwi-like subfamily found in
                     eukaryotes. This domain is found in Piwi and closely
                     related proteins, where it is believed to perform a
                     crucial role in germline cells, via RNA silencing. RNA
                     silencing refers to a group of related...; cd04658"
                     /db_xref="CDD:240016"
     Site            order(521,525,537..540,543,553,556,560,564)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240016"
     Site            order(585,587,656,790)
                     /site_type="active"
                     /db_xref="CDD:240016"
     CDS             1..815
                     /gene="LOC144114353"
                     /coded_by="XM_077648015.1:1..2448"
                     /db_xref="GeneID:144114353"
ORIGIN      
        1 mafynryesl rpscvshqfq fapadtpgds gdggrpvgrr hrdefavvet rpahidnkrg
       61 tsgspinvva nyirlismpq fcihqyhvef tptvessrmr rallvdhmhm fnkclvfdgm
      121 sdlksptrlq qditevfpqr rtdgmiicvr fkwvqelapt hpellrlfnt qmrrnlerld
      181 fvqinrhffd rcavsripqh glelwqglvt aigqydsgvm lvtdtlhkvl rrdsvydlms
      241 qiehvsnykd ecvkrvagcf vmtpynnrty rvddidwdkn pactfetkeg pktyadyyrd
      301 hykkhirdmc qpllvvrpkd kdlragrtqn iylvpglcfl tgltdemran vtvmrdlaqh
      361 trvepskrwr nlfvfmerin nneairndmd swgvrfddsl vsinarvlqp ekvmqgsnty
      421 rysaatgdfs retrdrplhv avaveswivl chrceeanvt efvrtlmsvc ppmgvkirqp
      481 llvlldddrp sgfvyalrql asggnielal ivlpnsrkdr ydiikkeacv dlglhtqvil
      541 trtisnrknv rsvatkvavq lncklggeaw cleiplvstm vigydtypds sapnrsagaf
      601 vasmnksltr wyqrvsfydt heglgkslat llrdalrkys qcndgaspdr iiffrdgvsd
      661 gqipqvkvwe ideilaslqa lfpgvehklg fvvvtkrist rffvsgrehv tnpppgtvvd
      721 sevtrperyd fflvsqsvrq gtvaptqynv iydttglkpd hmqrlayklt hlyfnwpgti
      781 rvpapcqyah klvflagqsl haehnprlfs slfyl
//
LOCUS       XP_077504179             121 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114369 [Amblyomma americanum].
ACCESSION   XP_077504179
VERSION     XP_077504179.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648053.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 12% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..121
                     /product="uncharacterized protein LOC144114369"
                     /calculated_mol_wt=12295
     CDS             1..121
                     /gene="LOC144114369"
                     /coded_by="XM_077648053.1:1..366"
                     /db_xref="GeneID:144114369"
ORIGIN      
        1 mptacpgqgs svhakglsta sassrspasl dqthqytpnl taggrdsspa grectlcgap
       61 hatldhilfg cpadpppqgq paigtweewe allasqnpdt qlrcvnrgas amalrgldtc
      121 a
//
LOCUS       XP_077504227             888 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077504227
VERSION     XP_077504227.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648101.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 16% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..888
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=98827
     Region          128..861
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     CDS             1..888
                     /gene="LOC144114390"
                     /coded_by="XM_077648101.1:1..2667"
                     /db_xref="GeneID:144114390"
ORIGIN      
        1 mcespciaaq qgqaatsssp spvpsasggn qatasppgps rvsfeqalqq gvaaspqpra
       61 vsgsqtpaae rsaqpsveps gyrpsygaaa gaanvaassp wpasaaaavs gcqpaaaapp
      121 lsppctspgd dvkieelert lpshfprrpa hgqlgrtiql lanhfsieip tgsvyhydvd
      181 issetvqetk vpeqkkyrcl stkinrivie llvkkyrqdl ancipafdgh knlytrrqln
      241 frertftvdl eedqrsqkfi ikiqyaatvn lealhgvfek rvqtvpqevl qaidivlrhs
      301 psinlapvgr sffrppgpne hndlgggrev wfgyytsvrp aqwkpmlnvd msatafyesl
      361 plvdfmcrfl sdsrrvltpa dfrslrdnqy vrlnrelrvk vthlpyprky kvvkitrepa
      421 kdiyfesegs qisvadyfqs ryrrlsypnf pcvqsgspth pvyiplevce laegehcrkk
      481 ldesqtaemi krtakppakr fheirqsvrd mvsssdkylr efgvkintep sqvvgrvldp
      541 pslvfenntm ckprdgtwdl qgqrfykams mtqwivlnvs cfahkdclen fikmlirigq
      601 elgmhiaqpl avitfdpnrk amrtvlteqr kqhpqlemvv avitkatnca eikqvaetel
      661 slrtqcildn navqkcnaal iqnlcqkina kmgginnsll mpekpkllhr pvivigadvs
      721 hpspgdkvrp siavcvgsld svpskfhati rvqiedseak trveiikdlk dmmkelllvf
      781 yrttghkper ivfyrdgvse gqflevrnre vsvtrlacae msptetyeps ltftvvqkrr
      841 hprfmpands dgvgkfrnap psttvvfvvv ssntnysivi ssvivanl
//
LOCUS       XP_077504242             121 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114393 [Amblyomma americanum].
ACCESSION   XP_077504242
VERSION     XP_077504242.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648116.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 15% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..121
                     /product="uncharacterized protein LOC144114393"
                     /calculated_mol_wt=13135
     CDS             1..121
                     /gene="LOC144114393"
                     /coded_by="XM_077648116.1:1..366"
                     /db_xref="GeneID:144114393"
ORIGIN      
        1 mtsfrdqrgl virlrashgl llqslalaas alhliqatry llkhkpggda sdaaeeatcg
       61 flietriarf ksrpppvppv pvrldssalq trrdstavaa rrestavpsr ressvtypey
      121 l
//
LOCUS       XP_077504252             278 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114398 [Amblyomma americanum].
ACCESSION   XP_077504252
VERSION     XP_077504252.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648126.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..278
                     /product="uncharacterized protein LOC144114398"
                     /calculated_mol_wt=32055
     Region          64..>132
                     /region_name="CwlO1"
                     /note="Uncharacterized N-terminal coiled-coil domain of
                     peptidoglycan hydrolase CwlO [Function unknown]; COG3883"
                     /db_xref="CDD:443091"
     CDS             1..278
                     /gene="LOC144114398"
                     /coded_by="XM_077648126.1:1..837"
                     /db_xref="GeneID:144114398"
ORIGIN      
        1 mrfaqnpfsf ivqvrkqyge cfcssdpcli vlpcprllsf vfcmtsclrk llllggdiet
       61 npgpdmaqia kqlkeiasdi kdikenkldd iekkldaltr lensvasfhq qlanmdcvik
      121 klekkiddln nrsrrsnlii yglpendget ndslestvnk eiiqdtleld pvaierihrf
      181 grpgpdktrp vifklldtrq ksailknchk lketeysige dfslkirelr kklwekakph
      241 rdkrekvsma fdklyinsri fvwddeindi vpfkktti
//
LOCUS       XP_077504304             179 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114435 [Amblyomma americanum].
ACCESSION   XP_077504304
VERSION     XP_077504304.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648178.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..179
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..179
                     /product="uncharacterized protein LOC144114435"
                     /calculated_mol_wt=20088
     CDS             1..179
                     /gene="LOC144114435"
                     /coded_by="XM_077648178.1:18..557"
                     /db_xref="GeneID:144114435"
ORIGIN      
        1 mdivkppepl qfsgnlrrnw htfkqklelf ftatpttppr seaaktaill saageealdv
       61 ynnfsfeaed grspgellqg rrlrtslpey nedssvpvrk hcqssrgkvl pplkegssvr
      121 vhgdnwsrta tviekaaprs yvvktenqrl lrrnrqhllh ipgsdesmqr dqdvdslgk
//
LOCUS       XP_077504315             509 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114441 [Amblyomma americanum].
ACCESSION   XP_077504315
VERSION     XP_077504315.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648189.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..509
                     /product="uncharacterized protein LOC144114441"
                     /calculated_mol_wt=56832
     Region          4..97
                     /region_name="THAP"
                     /note="The THAP domain is a putative DNA-binding domain
                     (DBD) and probably also binds a zinc ion; smart00980"
                     /db_xref="CDD:214951"
     Region          239..291
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          321..489
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..509
                     /gene="LOC144114441"
                     /coded_by="XM_077648189.1:120..1649"
                     /db_xref="GeneID:144114441"
ORIGIN      
        1 mvlcaivgcs nrsdssrrkk kptntnfyri priiadkcer sktlstnrrt lwlsrikrad
       61 ldpdnpnfrv cgahfttgap adlfdntnpd wapslhlgys trpvgsdrhd rsqqrrqrkr
      121 caetemtgse epcgvaapvp dvppsphqae evdgageetg vyvqtdmgms digaleqeci
      181 rlndcvyttr sekvqlemte ellrssepkv vfytgiasft tllaifkmle pfvshsvnns
      241 lakfqefvlf lmkiklnlqn adlafrfnvs estvsrifdk wlhvahcrlk sqvmwpersa
      301 lqrtmpqafy dsfgsdvavi idcfeikier pssylprset wstykgsnta kyligiapqg
      361 ivtfisegwg grvsdkhite hcglldnllp gdvvladrgf nvadsvgfyr arlhlpaytk
      421 gkkqlsaaev gstrklanvr ihvervigli rnkflllksv lpidyvtcrq gdsitpldki
      481 vtvccalsnl cpsivaalke psrecaric
//
LOCUS       XP_077504324             306 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-(1,3)-fucosyltransferase fut-1-like [Amblyomma americanum].
ACCESSION   XP_077504324
VERSION     XP_077504324.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648198.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..306
                     /product="alpha-(1,3)-fucosyltransferase fut-1-like"
                     /calculated_mol_wt=35005
     Region          34..221
                     /region_name="Glyco_transf_10"
                     /note="Glycosyltransferase family 10 (fucosyltransferase)
                     C-term; pfam00852"
                     /db_xref="CDD:459962"
     CDS             1..306
                     /gene="LOC144114451"
                     /coded_by="XM_077648198.1:1..921"
                     /db_xref="GeneID:144114451"
ORIGIN      
        1 mslrrdadvv apyknftsyd eervlpvnyl atailrkpkt aalfvsgcee plrnntdgiy
       61 vwdtphsten ylkhinhtld vyifsdcgrp lcnsrmdcie maarefyfif vlesspcfdh
      121 pleliydsfa wdivpvyfgq sslgdvvppg svydttpert sfhivdklni lrdniteylp
      181 yltwkevyyp tevenplcal cdalyrtpea hsshhkdvme wwdhrqncgy vypspghpmi
      241 vveytqdgas vepdeiadip grirrtpppp rppvttkycc eyedsspkne ensagnaveh
      301 ppheml
//
LOCUS       XP_077504335             391 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-(1,3)-fucosyltransferase C-like [Amblyomma americanum].
ACCESSION   XP_077504335
VERSION     XP_077504335.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648209.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..391
                     /product="alpha-(1,3)-fucosyltransferase C-like"
                     /calculated_mol_wt=45033
     Region          35..151
                     /region_name="Glyco_tran_10_N"
                     /note="Fucosyltransferase, N-terminal; pfam17039"
                     /db_xref="CDD:465336"
     Region          171..354
                     /region_name="Glyco_transf_10"
                     /note="Glycosyltransferase family 10 (fucosyltransferase)
                     C-term; pfam00852"
                     /db_xref="CDD:459962"
     CDS             1..391
                     /gene="LOC144114460"
                     /coded_by="XM_077648209.1:1..1176"
                     /db_xref="GeneID:144114460"
ORIGIN      
        1 mrykkeveea smfytrwrpw ygrdlpgvyl ddevdnpril lwsvptlewe kdeplpahge
       61 rvlqacpdrd dgrtcfvnrk rhylgradai lfdadnlnyy dypalrhrgq vwviwtrgkp
      121 plnpsydlls maakfnwtms yrgdadvtvp ysmwapppmg lsyeslnqti rkkktvgvwl
      181 vseceddrla elalvkleei rsdfelarfv alatssriik ncggdecknr heclknfqnq
      241 yfflfvvess pcfehpheim fdalhysiip iyfgssslgs tvppfsvfdt taaasaveav
      301 gailniyvry eryldcmkwk ekyriaapgn dlcalcdaly enrnrttndd vvqwwkrgsd
      361 ckilpesrrr aftsapgspf ssspnqasrl q
//
LOCUS       XP_077504346             191 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-(1,3)-fucosyltransferase C-like [Amblyomma americanum].
ACCESSION   XP_077504346
VERSION     XP_077504346.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648220.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..191
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..191
                     /product="alpha-(1,3)-fucosyltransferase C-like"
                     /calculated_mol_wt=21390
     Region          <5..21
                     /region_name="Glyco_tran_10_N"
                     /note="Fucosyltransferase, N-terminal; pfam17039"
                     /db_xref="CDD:465336"
     CDS             1..191
                     /gene="LOC144114467"
                     /coded_by="XM_077648220.1:1..576"
                     /db_xref="GeneID:144114467"
ORIGIN      
        1 mnakfnwtms hrgdadvtvp yrmwappsmg fsyedlnqti nkkssaavwl vseceakriv
       61 eanvprvddi kndfeyasfv arltdaniis gcggvecknr heclerfqne yffllvvess
      121 pcfehphqim fdalqysiip iyfgssslgs tvppfsvfdt taaasvldvv ereklsdqla
      181 lerqaedfpg i
//
LOCUS       XP_077504359              94 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114475 [Amblyomma americanum].
ACCESSION   XP_077504359
VERSION     XP_077504359.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648233.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..94
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..94
                     /product="uncharacterized protein LOC144114475"
                     /calculated_mol_wt=10179
     CDS             1..94
                     /gene="LOC144114475"
                     /coded_by="XM_077648233.1:1..285"
                     /db_xref="GeneID:144114475"
ORIGIN      
        1 mnsqlvvlaa llacalaglh hhggghyhhf hhyggygheg fdygpklvdv ryvkapvtri
       61 kyvskpvfgi vkvpvakist vikpvvhvdh vpaw
//
LOCUS       XP_077504373            1377 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114481 [Amblyomma americanum].
ACCESSION   XP_077504373
VERSION     XP_077504373.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648247.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1377
                     /product="uncharacterized protein LOC144114481"
                     /calculated_mol_wt=152316
     Region          <32..>235
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Region          <182..>312
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Region          1084..1250
                     /region_name="Innate_immun"
                     /note="Invertebrate innate immunity transcript family;
                     pfam12782"
                     /db_xref="CDD:432781"
     CDS             1..1377
                     /gene="LOC144114481"
                     /coded_by="XM_077648247.1:1..4134"
                     /db_xref="GeneID:144114481"
ORIGIN      
        1 mvragvvrwl sslksqqlvr rphkprvlte gpaspplnit arptgpnsik isweppkeed
       61 wnghlkgyyi syrpvgsseq hyhktvevhn phqrqeihlt nlrlsmsysv aiqaftgkgt
      121 gpmsrevlvk tlddvppspp slevvsvsts svtlgwslkt sfgnpvteyv lhqrkeadhw
      181 qetpisttqp vytvrdlecg ttyqfymtah nslgrsepsd viraktdgaa plsptkeefi
      241 qasqrhasls lrswksggce lldftvrlrq gpaqawstva eglpanqsql llrnltpgat
      301 ynvhvvarst agateaqyef ttpngtahva sveatssqpk rsslpsmtdl eilvpilvss
      361 cvvlivivvg cilcsreslc sdrhcgrpdl ratyseevvd mkdlantaec marcedgvhh
      421 sasqmnsrfp agqsiyaqrp vveteddrhp yampydvlhg gggggggaae gatadegtad
      481 gascqnhldg rntlkrrsdl kdhrsnniyi srqvglvcma lvqhshggeq rhpssfemss
      541 sgvsknqldl akellrlfnl hhvlasmnvs rsaqmksgse gsfvpsnpyw ilmnhtqrrq
      601 papqsfvsaa nrthankptg ydtkipsrag krnyttgdka sltysmlkah gpekrpssey
      661 rrnytaprak nqtfvaplpm ekftfedapk srpllhtlhk evldekiera ktsfpterth
      721 garakirsse idseaaasdf klqpamriit spsssikhei pvsfnhafiv lgqpaerkka
      781 lasdyevsvl gavlgdlidq sesghkrdkt rqykhvfnig dkpviqitev snthseqask
      841 lntsavkeel siksavhkhw ssprmlahkk vaasgvltls hssptaphgd kkhhfeqpys
      901 kvitprmgdi slgasaself qttykpisqh pssqfayrrl rqesagtgph ifnlrsqhda
      961 pqsqwiplkk sfvkrdvslk ndaiakapnv taalsalldd tntikpgkkw rgilkpryye
     1021 tlnftvpkll peviftpdlr gtpissispf desnigsvhr vvtiygkntn hkplapvtvv
     1081 vaegsqpast vmqqtslvkr rhdepdppkp kyihslsdkf gerppppdrk ddyllhkrak
     1141 gfrgdgeagr pplgggrrqs plepkfdpef ersrflkppp lfperdrpah hgrhppddhp
     1201 rhrdgppftv yghrhpdkhy gngeppyrpg krpyhdvpdr seglppredg dfeedldpsr
     1261 fddyeawrhl trpplpasgf qppfmpgfgg yppfrgppfp gggppfmgsp gigtilpgkk
     1321 mekfkyllkg gfrgsgglrp sffaapqgwy cpcnyyppag rfithgypsf wgtmrgd
//
LOCUS       XP_077504400            1403 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114492 [Amblyomma americanum].
ACCESSION   XP_077504400
VERSION     XP_077504400.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648274.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1403
                     /product="uncharacterized protein LOC144114492"
                     /calculated_mol_wt=157785
     Region          432..992
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1403
                     /gene="LOC144114492"
                     /coded_by="XM_077648274.1:1..4212"
                     /db_xref="GeneID:144114492"
ORIGIN      
        1 mgdslallav wkeqttgidq nvsllqrfft tidiplrarf hissvaqqpg thteeryyqa
       61 vckytfpdtg sravptsalq avsarvtqqw cllgrmmpfr ghlqwliper ergfwcrwmp
      121 headiqlasl sfgtftglcl faqshvfysk pgapgytlsc afkhderllq vmlklvhkkg
      181 cshtcssykl qipyhsifrv vlneaetyhd algiylqlvt vpllyrsayd vneweastgd
      241 gvwydrvfql gcpcwwvmrs ydiggssvvk ltlrnklkar qaisrlcarc kpettfqysp
      301 mltkkvgakl reikmrchsk ikpnlcfptc yafsavlqqg hdgfaqmall acekleefee
      361 llirllhtsg palelalyai rsaieeheiv diisclpklf akfctpaaal sktpagtclv
      421 rrcivtpskv illppqihcq nrilrkfdpe fsrrvsfrdd nmqnlsyslm sghcrhmvie
      481 rvvartlrdg lvagnrrfrl latscsqlrd hgawlyatda kgyssemiry wmgdfsgias
      541 takkmarmgq cfssteesvh vpllsnsvre vpdilggkhp vtaehyifsd gigmispgll
      601 kevtdkiglt vspsaiqiry agykgmlcvn pllsgrqivl rksmrkfncv nsecieviki
      661 saprpvflnr qlitlleqlg vpsrvffclq qrmvtlftea lvsdsvalqv letyvgpalp
      721 fgqllrhgfs ltrdpfvrsi lfavyrtsme sllsktriav prnlgrnmfg vldetgtlky
      781 gqvfvqftsl gsstrqqngp pvfilsgtvl vtkcpclhpg dvrkftavnv palhhikdcv
      841 vfpangfrph sdemagsdld gdeyiviwdr nlffpghnqe pmiygqkvqq qrselslvdg
      901 makfisdfik ndnvgvmsna hlamadklkd gvfskqclsi aakistcldf aktgvaavle
      961 kherpreypd fmakgchknt yrsnrvlghl yrfqrflesv vstsfnsqlv dgrsniklle
     1021 fhgwmsyrsv veelraayes dmdrilkqyg ikteaevvsg findtssfnk snyeksnvev
     1081 lvtrqyraia dstrkrffkk veeacylesa fsteeqttil lrmasacfmv tysnaqktcs
     1141 sfpwifseli llvmasasrr empyrqgnll iacldsqlpp assqlsaksi alevvlkwav
     1201 kedlllqdag vrcaricrhc letlfinsar aedaiangeg apspgcyvks ffryvgstsv
     1261 vfpkcdscsw ssqthtltma alrtysllai fcdlchlglp cdarlhepvq eiqegndvri
     1321 kvtspgfqdm llhgqdevvs llavwsgvhe vrirrgadrg ggrdivvssv grdwqrwfle
     1381 elllqpwlat avekrsleqf irg
//
LOCUS       XP_077504420             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114503 [Amblyomma americanum].
ACCESSION   XP_077504420
VERSION     XP_077504420.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648294.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 44% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144114503"
                     /calculated_mol_wt=13776
     CDS             1..127
                     /gene="LOC144114503"
                     /coded_by="XM_077648294.1:1..384"
                     /db_xref="GeneID:144114503"
ORIGIN      
        1 mddantdwvp tqhlgygpge rpavgmsarf artkarqkka aakaiadaat rqssedecta
       61 evtpvddpph escslrlesr aeiavqtdmt vqdiqavedd nkrlaaelra ttaekkqard
      121 arviisw
//
LOCUS       XP_077504441             238 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114516 [Amblyomma americanum].
ACCESSION   XP_077504441
VERSION     XP_077504441.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648315.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 28% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..238
                     /product="uncharacterized protein LOC144114516"
                     /calculated_mol_wt=25749
     Region          4..97
                     /region_name="THAP"
                     /note="The THAP domain is a putative DNA-binding domain
                     (DBD) and probably also binds a zinc ion; smart00980"
                     /db_xref="CDD:214951"
     CDS             1..238
                     /gene="LOC144114516"
                     /coded_by="XM_077648315.1:119..835"
                     /db_xref="GeneID:144114516"
ORIGIN      
        1 mvncavvgcs nrsdvtgrkk rptsarffsl pkvienqcer tktlsakrrs lwlarikran
       61 fnhespnvrv cgvhfikgep snlfdetnpd wapslslghg trhadsarhd rrakrlahkr
      121 radveatava seaspracpd spqppqaeev sadenetgia vqtdmttqdi qalekhsvll
      181 nehlgahlve paematasta apsvgarlrr cyatsaqgrs sataygirmk satsqngs
//
LOCUS       XP_077504454             351 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114524 [Amblyomma americanum].
ACCESSION   XP_077504454
VERSION     XP_077504454.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648328.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 48% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..351
                     /product="uncharacterized protein LOC144114524"
                     /calculated_mol_wt=39005
     Region          4..97
                     /region_name="THAP"
                     /note="The THAP domain is a putative DNA-binding domain
                     (DBD) and probably also binds a zinc ion; smart00980"
                     /db_xref="CDD:214951"
     CDS             1..351
                     /gene="LOC144114524"
                     /coded_by="XM_077648328.1:108..1163"
                     /db_xref="GeneID:144114524"
ORIGIN      
        1 mvncavvgcs nrsdvtgrkk rptsarffsl pkvienqcer tktlsakrrs lwlarikran
       61 fnhvspnvrv cgvhfikgep snlfdetnpd wapslslghg trhadsarhd rrakrlahkr
      121 radveaiava seaspracpd spqppqaeev sadenetgia vqtdmttqdi qaleqhsvvl
      181 nehlytlqke keqlenariq hhlvrtacel scfhiwtsen yrqqsrepsp ngvpgasskp
      241 ayqhnsapep setiieleew glncasqasl vhevlthepl psrheslpsr heshassqsv
      301 rslqapvgll eqeasvlhss artqskrkrr recaddalae hvgklrctad l
//
LOCUS       XP_077504466             387 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114531 [Amblyomma americanum].
ACCESSION   XP_077504466
VERSION     XP_077504466.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648340.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 31% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..387
                     /product="uncharacterized protein LOC144114531"
                     /calculated_mol_wt=41784
     Region          <198..>235
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     CDS             1..387
                     /gene="LOC144114531"
                     /coded_by="XM_077648340.1:1..1164"
                     /db_xref="GeneID:144114531"
ORIGIN      
        1 mtgsvgaata amsrrgnrff etdneyevil pslptgrivf ntvflhadvr arpyrvedfr
       61 dalahlkllp dvtalgayrm shvwavtfds ahavkkmlaa gemqvkerrc liidpanqdv
      121 rmkihwllpg vsdedvraaf vpygkvtdvk aerwrvqgvt dkgsttrlvs rslragvkld
      181 nfphqvsiag elalvvvpgr aplclrcrgk ghvrrdckip rcdacrrfgh eesecartya
      241 svtgrssneg asdlmmdeld aeeaatakgg nvletlvpls tsvaegsgrl epassvlqdg
      301 lpdekaavle daaarpneas enaesqqeem vvslaasssq agkrprekns daphtpeega
      361 eeppakapgv rrpscswsea svfqaaa
//
LOCUS       XP_077504475             192 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114542 [Amblyomma americanum].
ACCESSION   XP_077504475
VERSION     XP_077504475.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648349.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 43% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..192
                     /product="uncharacterized protein LOC144114542"
                     /calculated_mol_wt=21083
     CDS             1..192
                     /gene="LOC144114542"
                     /coded_by="XM_077648349.1:1..579"
                     /db_xref="GeneID:144114542"
ORIGIN      
        1 mddvntdwap tphlgygpge rpavgmsarf artkarqkka aakaiadaat rqssedecta
       61 evtpaddpph escslrlesr aeiavqtamt vqdiqaveed nkrlaaelra ttaekknkle
      121 msessfhgdd akvtfytgln sfpmlfaice lvechvkhta qnglgkfeef vvfpmklkwn
      181 fpvqglgyrf cv
//
LOCUS       XP_077504487             198 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114551 [Amblyomma americanum].
ACCESSION   XP_077504487
VERSION     XP_077504487.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648361.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..198
                     /product="uncharacterized protein LOC144114551"
                     /calculated_mol_wt=21578
     CDS             1..198
                     /gene="LOC144114551"
                     /coded_by="XM_077648361.1:1..597"
                     /db_xref="GeneID:144114551"
ORIGIN      
        1 mappccsilf pvnvveglrg adhdcvvivg tglnaehlkg dvlckyrepp aciqrgecas
       61 rrrlrtvdlk adeevfvlpl sddyairrlv ysptgpfnrd fddvrvfada aykglkrala
      121 agsakplvvl paqhgqfdgc dlatvlgafh alyvpleirq dipirkqkvl qlgfsvlpet
      181 klsfdktlla ahaielgr
//
LOCUS       XP_077504501             374 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114553 [Amblyomma americanum].
ACCESSION   XP_077504501
VERSION     XP_077504501.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648375.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..374
                     /product="uncharacterized protein LOC144114553"
                     /calculated_mol_wt=39065
     Region          30..113
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     CDS             1..374
                     /gene="LOC144114553"
                     /coded_by="XM_077648375.1:28..1152"
                     /db_xref="GeneID:144114553"
ORIGIN      
        1 mgsqqfclkw nnhqsnmlav fyqmlsneal vdvtlacegl slkahkmvls acspffqalf
       61 aenpckhpiv ilkdvryvdl raivefmyrg evvvsqedlm allktaetlk vrglvtsvdg
      121 aadtgpvgsp raeapplskr krsrrprrrs psdsnksdse dappatrikv pdspeiiddg
      181 smssdhmtdp htaspasras nvasstavps garhgssvqa sisqhtqdsi gddvgegdda
      241 dfdvepldvm eesattesvp vfpdvasssq algdesqqsh htgstsgnsa lvpaqpslps
      301 dipitslvdi epsptsltpy ddktiapvaa aspaaassgd gastsmaatd vsgvpaiagp
      361 sgyqqstpsh dppf
//
LOCUS       XP_077504535             774 aa            linear   INV 25-NOV-2025
DEFINITION  glutamate carboxypeptidase 2-like [Amblyomma americanum].
ACCESSION   XP_077504535
VERSION     XP_077504535.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648409.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..774
                     /product="glutamate carboxypeptidase 2-like"
                     /calculated_mol_wt=86069
     Region          213..390
                     /region_name="PA"
                     /note="Protease-associated (PA) domain. The PA domain is
                     an insert domain in a diverse fraction of proteases. The
                     significance of the PA domain to many of the proteins in
                     which it is inserted is undetermined. It may be a
                     protein-protein interaction domain. At...; cl28883"
                     /db_xref="CDD:333703"
     Site            308..310
                     /site_type="other"
                     /note="PA/protease or protease-like domain interface
                     [polypeptide binding]"
                     /db_xref="CDD:238300"
     Region          <401..632
                     /region_name="Zinc_peptidase_like"
                     /note="Zinc peptidases M18, M20, M28, and M42; cl14876"
                     /db_xref="CDD:472712"
     Region          661..769
                     /region_name="TFR_dimer"
                     /note="Transferrin receptor-like dimerization domain;
                     pfam04253"
                     /db_xref="CDD:461238"
     CDS             1..774
                     /gene="LOC144114572"
                     /coded_by="XM_077648409.1:1..2325"
                     /db_xref="GeneID:144114572"
ORIGIN      
        1 mavhaskecr svghckgsne qqtvkfskta krdsdsfsss ecqskprmap fhprysrtpa
       61 ermllnwvlc liafagvttv smlfvlmnvy qspllgprsr elaelygkit adtysaedgf
      121 ndallakitd pdvvhmlraf evaknpvdal qtvynywsni gmdilkvkky rvlrswshqv
      181 knntvkmyks dsllveakst dspsntskqn yrpaflaysp prdvngtfvy vnyaeesdfe
      241 flkknnidva griclvrvgk itksqqvmna qarhalgvvf flsmedvrva rdrnvtfpen
      301 vwvgdnalqh gtvasalgdp ltpgypsegt lhrltvqdag lpfvpaqpin eedaqaifqh
      361 itevacppew dphlnvpcgi vsepnvklql ttynqmkisv iqdvigvwkg siepdrlvvv
      421 gsrttgsata tirehrwlav tqlllqslaf rhlrrigwrp rrslafalwq tdqssalgst
      481 ewvdehagql rqqavlyvdn npvtgalfep haspslapsl revanmvylk gsdstlkaaw
      541 taqnstpkdd rgeplplavn ghwddgpfaf avgvptlavs fgepdrpyge wpahrvlpda
      601 aqqddvvhks viaaqvgalm arlwadstvl pydpvalanw lldaldrfer rhghvletcd
      661 lslgsakrls ktfhsvaasf mrraqevtra nplvtrmind iltglekvfa iprgvptrhf
      721 ahrsilyspe glpfpgledl vrqtpidkta frehlallme siiqatelmi eypf
//
LOCUS       XP_077504555             152 aa            linear   INV 25-NOV-2025
DEFINITION  protein NYNRIN-like [Amblyomma americanum].
ACCESSION   XP_077504555
VERSION     XP_077504555.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648429.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..152
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..152
                     /product="protein NYNRIN-like"
                     /calculated_mol_wt=17053
     Region          12..100
                     /region_name="rve"
                     /note="Integrase core domain; pfam00665"
                     /db_xref="CDD:459897"
     CDS             1..152
                     /gene="LOC144114588"
                     /coded_by="XM_077648429.1:1..459"
                     /db_xref="GeneID:144114588"
ORIGIN      
        1 mdsqlyivvv qvkpgpfppa gpyrycliai drytrwpeaw plegitaedv alaffagwia
       61 rcrpphcvtt dqgrqfeshl frllgltigf ersrttsyhp cangmiehfh rqfkaaimch
      121 pdstwleaip avtlglratf kpdiqatpak lv
//
LOCUS       XP_077504568             175 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114594 [Amblyomma americanum].
ACCESSION   XP_077504568
VERSION     XP_077504568.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648442.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..175
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..175
                     /product="uncharacterized protein LOC144114594"
                     /calculated_mol_wt=18670
     CDS             1..175
                     /gene="LOC144114594"
                     /coded_by="XM_077648442.1:1..528"
                     /db_xref="GeneID:144114594"
ORIGIN      
        1 mnlrfsgiai aasrtyllhd cphwgapana pcfgithihf ceeflgtdng grpaegsiya
       61 vrgscsasqg nvratstrss gsveqlarar ernasstaaa fdssmdgtpv haratagdqt
      121 cddgrrkprq hiakggainq ypgsdsckdg kcshqqwfps hrrfvfreed daage
//
LOCUS       XP_077504579             149 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114599 [Amblyomma americanum].
ACCESSION   XP_077504579
VERSION     XP_077504579.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648453.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..149
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..149
                     /product="uncharacterized protein LOC144114599"
                     /calculated_mol_wt=15641
     CDS             1..149
                     /gene="LOC144114599"
                     /coded_by="XM_077648453.1:1..450"
                     /db_xref="GeneID:144114599"
ORIGIN      
        1 mpqmfapkgh wgapanapcf githihfcee flgtdnggrp aegsiyavrg scsasqgnvc
       61 atstrssgsv eqlararern asstaaafds smdgtpvhar atagdqtcdd grrkprqhia
      121 kggainqypg sdpckdgkcs hqqwfpshh
//
LOCUS       XP_077504589             143 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114608 [Amblyomma americanum].
ACCESSION   XP_077504589
VERSION     XP_077504589.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648463.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..143
                     /product="uncharacterized protein LOC144114608"
                     /calculated_mol_wt=14970
     CDS             1..143
                     /gene="LOC144114608"
                     /coded_by="XM_077648463.1:1..432"
                     /db_xref="GeneID:144114608"
ORIGIN      
        1 meghwgapan apcfgithih fceeflgtdn ggrpaegsiy avrgscsasq gnvcatstrs
       61 sgsveqlara rernasstaa afdssmdgtp vharatagdq tcddgrrkpr qhiakggain
      121 qypgsdpckd gkcshqqwfp shh
//
LOCUS       XP_077504602             421 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114617 [Amblyomma americanum].
ACCESSION   XP_077504602
VERSION     XP_077504602.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648476.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..421
                     /product="uncharacterized protein LOC144114617"
                     /calculated_mol_wt=49889
     Region          14..>153
                     /region_name="PRP38"
                     /note="PRP38 family; pfam03371"
                     /db_xref="CDD:460901"
     CDS             1..421
                     /gene="LOC144114617"
                     /coded_by="XM_077648476.1:159..1424"
                     /db_xref="GeneID:144114617"
ORIGIN      
        1 mapggpadkk nnvlplwgne ktmnlnnlil tnilsspyfk vnlyklktyh evvdeiyynv
       61 qhlepwekgs rktsgqtgmc ggvrgvgagg ivstafcily klftlkltrk qlvglmnhcd
      121 spyiralgfm yirftqppad lvdwyepyld dderwlqghl rgvcamhpys lyvvpgwlrp
      181 hthtclalei gtfqsmycfh tclshepscp kcpeapthga spscrepprd sswdrerqri
      241 rrsrsrerhr ehhhrsrspr dvrlyrshdr prderereer srddrprder prderlrddr
      301 srddrsrddk srddrsrddr srddrsrddr srddrsrddr srddrsrsdr srddrarddr
      361 lrdeksrese rrkesgsyry rsspehskhg drprehsssh dhrtssrker syspaarnhh
      421 k
//
LOCUS       XP_077504614             115 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114625 [Amblyomma americanum].
ACCESSION   XP_077504614
VERSION     XP_077504614.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648488.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..115
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..115
                     /product="uncharacterized protein LOC144114625"
                     /calculated_mol_wt=12142
     CDS             1..115
                     /gene="LOC144114625"
                     /coded_by="XM_077648488.1:1..348"
                     /db_xref="GeneID:144114625"
ORIGIN      
        1 mprstagtvp seragglgwn parkevpsrs mgcllancsi ssaspevqgs gcthsgsrsp
       61 srllrrgkma ashtpcrapr reqslhrlrn dnvqlrgqnp artevpgrim gcllg
//
LOCUS       XP_077504667             489 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114658 [Amblyomma americanum].
ACCESSION   XP_077504667
VERSION     XP_077504667.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648541.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..489
                     /product="uncharacterized protein LOC144114658"
                     /calculated_mol_wt=55265
     Region          297..380
                     /region_name="retropepsin_like_LTR_1"
                     /note="Retropepsins_like_LTR; pepsin-like aspartate
                     protease from retrotransposons with long terminal repeats;
                     cd05481"
                     /db_xref="CDD:133148"
     Site            305..307
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133148"
     Site            305
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133148"
     CDS             1..489
                     /gene="LOC144114658"
                     /coded_by="XM_077648541.1:1..1470"
                     /db_xref="GeneID:144114658"
ORIGIN      
        1 mtelpppepl rfganvadnw trfkqrvely fiatestepg rqrsnaqkaa illhlagqea
       61 idvfntfdlt aedkkdydkl vqafeaywlp qsnetferyv frnrtqaege pfeqfyrdlq
      121 lksktcnfdn lresmlrdqi vygtssktlr ekllakkdlt lttavewaka eeitaaqskv
      181 wsedksvdav geeknkhqqh rndalgygkg rhqekkcgyc nrshrprecp aygkkcarcg
      241 rqnhfavcck araadvneva erpdddddft vlhvstackk asaadardwe vatrisgrev
      301 rlkvdtgaqa nllprglfea lgtkqqpepa ravlrsyggn eivhmgkvcl pvrigadtrl
      361 veffvvkkgr qailglqace qfglvnrira vkkdealsec vrrqypklft gigklkreys
      421 mtlqdgarpv aeparrvpha irsrlkeeld rleaagiiak vrepsdwvsp lviarknngq
      481 lrvcmapkk
//
LOCUS       XP_077504677            1316 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114667 [Amblyomma americanum].
ACCESSION   XP_077504677
VERSION     XP_077504677.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648551.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 15% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1316
                     /product="uncharacterized protein LOC144114667"
                     /calculated_mol_wt=145695
     Region          148..831
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          1126..>1309
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     CDS             1..1316
                     /gene="LOC144114667"
                     /coded_by="XM_077648551.1:1..3951"
                     /db_xref="GeneID:144114667"
ORIGIN      
        1 mdaewvddtk lwpklqrpsv psasggnqat ppapspagps wvsfaqalqq gaaaspqpra
       61 vsggpttaad psahptaqps asrpsygaaa aagsakvass sprpasaaaa vsgtqpaaaa
      121 lppsppstpp vavtqqtpaa gdqatsgdvv rikelertlp shfprrpahg qlgrtiqlla
      181 nhfsieiptg svyhydvdis setaketkvp eqkkyrclst kinriviell vkkyrqdlan
      241 cipafdgrkn lytrrqlnfr ertftvdlee dqrsqkfiik iqyaatvnle ilhgvfqkrv
      301 qtvpqevlqa ihivlrhsps inlapvgrsf frppgpneyn dlgggrevwf gyytsvrpaq
      361 wkpmlnvdms attfyeslpl vdfmcrflsd srrvltpadf rslrdnqyvr lnrelkglrv
      421 kvthlpyprk ykvvkitrep akeiyfeseg sqisvadyfq sryrrlsypn fpcvqsgspt
      481 hpvyiplevc elaegqhcrk kldesqtaem ikrtakppak rfqkirqsvr dmvsssdkyl
      541 refgvkinte ptqvvgrvld ppslvfennt mckprdgtwd lrgqrfykam smtkwivlnv
      601 srfahkdsle nfirmlirig qelgmriaqp lavitfdtnr kpmrtvlteq rkqypqlemv
      661 vavitkatny aeikqvaete lslrtqcild nnvvqkcnaa liqnlcqkin akmgginnsl
      721 lmqekpklfh rpvivigadv shpspgdkvr psiaacvgsl dsvpskfhat irvqiedska
      781 karveiirdl kdmikellla fhhatrhkpe rivfyrdgvs egqflevrny eswrhsvcqp
      841 cydgaaakrs hcpldkepfq eedmawttis rgnvlgrkvr rwnsehgcda ecvasamleh
      901 fanayqyham ispcswrhsv cqpcydgaaa krshcpldke pfqeedmawt tisrgnvlgr
      961 kvrrwnsehg cdaecvasam lehfanayqy hamispsvps asggnqatpp apspagpswv
     1021 sfaqalqqga aaspqpravs ggpttaadps ahptaqpsas rpsygaaaaa gsakvasssp
     1081 rpasaaaavs gtqpaaaalp psppstppva vtqqtpaagd qatsgdvvri kelertlpsh
     1141 fprrpahgql grtiqllanh fsieiptgsv yhydvdisse taketkvpeq kkyrclstki
     1201 nriviellvk kyrqdlanci pafdgrknly trrqlnfrer tftvdleedq rsqkfiikiq
     1261 yaatvnleil hgvfqkrvqt vpqevlqaih ivlrhspsin lapvgrsffr ppgpne
//
LOCUS       XP_077504688            2746 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114676 [Amblyomma americanum].
ACCESSION   XP_077504688
VERSION     XP_077504688.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648562.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 18% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2746
                     /product="uncharacterized protein LOC144114676"
                     /calculated_mol_wt=307349
     Region          31..69
                     /region_name="zf-C3HC4_3"
                     /note="Zinc finger, C3HC4 type (RING finger); pfam13920"
                     /db_xref="CDD:464042"
     Region          274..>938
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          1150..>1814
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          2071..2745
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     CDS             1..2746
                     /gene="LOC144114676"
                     /coded_by="XM_077648562.1:1..8241"
                     /db_xref="GeneID:144114676"
ORIGIN      
        1 maptslqtvf gfdrgfdwrp tcfvnpfppt rvcivcglvp ssaamlpclh llcqscsega
       61 gakrnhcpfh kepfqeedvv wsaftrdsil grkvrcwnae hgcdaevfas amlehfanac
      121 qfhvvrcpec sesvrhrdia ehlapvpsas ggnqatppap spagpswvsf aqalqqgaaa
      181 spqpravsgg pttaadpsah ptaqpsasrp sygaaaaags akvasssprp asaaaavsgt
      241 qpaaaalpps ppstppvavt qqtpaagdqa tsgdvvrike lertlpshfp rrpahgqlgr
      301 tiqllanhfs ieiptgsvyh ydvdisseta ketkvpeqkk yrclstkinr iviellvkky
      361 rqdlancipa fdgrknlytr rqlnfrertf tvdleedqrs qkfiikiqya atvnleilhg
      421 vfqkrvqtvp qevlqaihiv lrhspsinla pvgrsffrpp gpneyndlgg grevwfgyyt
      481 svrpaqwkpm lnvdmsattf yeslplvdfm crflsdsrrv ltpadfrslr dnqyvrlnre
      541 lkglrvkvth lpyprkykvv kitrepakei yfesegsqis vadyfqsryr rlsypnfpcv
      601 qsgspthpvy iplevcelae gqhcrkklde sqtaemikrt akppakrfqk irqsvrdmvs
      661 ssdkylrefg vkinteptqv vgrvldppsl vfenntmckp rdgtwdlrgq rfykamsmtk
      721 wivlnvsrfa hkdslenfir mlirigqelg mriaqplavi tfdtnrkpmr tvlteqrkqy
      781 pqlemvvavi tkatnyaeik qvaetelslr tqcildnnvv qkcnaaliqn lcqkinakmg
      841 ginnsllmqe kpklfhrpvi vigadvshps pgdkvrpsia acvgsldsvp skfhatirvq
      901 iedskakarv eiirdlkdmi kelllafhha trhkperisw rhsvcqpcyd gaaakrshcp
      961 ldkepfqeed mawttisrgn vlgrkvrrwn sehgcdaecv asamlehfan ayqyhamisp
     1021 svpsasggnq atppapspag pswvsfaqal qqgaaaspqp ravsggptta adpsahptaq
     1081 psasrpsyga aaaagsakva sssprpasaa aavsgtqpaa aalppsppst ppvavtqqtp
     1141 aagdqatsgd vvrikelert lpshfprrpa hgqlgrtiql lanhfsieip tgsvyhydvd
     1201 issetaketk vpeqkkyrcl stkinrivie llvkkyrqdl ancipafdgr knlytrrqln
     1261 frertftvdl eedqrsqkfi ikiqyaatvn leilhgvfqk rvqtvpqevl qaihivlrhs
     1321 psinlapvgr sffrppgpne yndlgggrev wfgyytsvrp aqwkpmlnvd msattfyesl
     1381 plvdfmcrfl sdsrrvltpa dfrslrdnqy vrlnrelkgl rvkvthlpyp rkykvvkitr
     1441 epakeiyfes egsqisvady fqsryrrlsy pnfpcvqsgs pthpvyiple vcelaegqhc
     1501 rkkldesqta emikrtakpp akrfqkirqs vrdmvsssdk ylrefgvkin teptqvvgrv
     1561 ldppslvfen ntmckprdgt wdlrgqrfyk amsmtkwivl nvsrfahkds lenfirmlir
     1621 igqelgmria qplavitfdt nrkpmrtvlt eqrkqypqle mvvavitkat nyaeikqvae
     1681 telslrtqci ldnnvvqkcn aaliqnlcqk inakmgginn sllmqekpkl fhrpviviga
     1741 dvshpspgdk vrpsiaacvg sldsvpskfh atirvqieds kakarveiir dlkdmikell
     1801 lafhhatrhk periswrhsv cqpcydgaaa krshcpldke pfqeedmawt tisrgnvlgr
     1861 kvrrwnsehg cdaecvasam lehfanayqy hamispcswr hsvcqpcydg aaakrshcpl
     1921 dkepfqeedm awttisrgnv lgrkvrrwns ehgcdaecva samlehfant yqyhamispc
     1981 pravsggptt aadpsahpta epsasrpsyg aaaaagsakv asssprpasa aaavsgtqpa
     2041 aaalppspps tppvavtqqt paagdqatsg dvvrikeler tlpshfprrp ahgqlgrtiq
     2101 llanhfsiei ptgsvyhydv dissetaket kvpeqkkyrc lstkinrivi ellvkkyrqd
     2161 lancipafdg rknlytrrql nfrertftvd leedqrsqkf iikiqyaatv nleilhgvfq
     2221 krvqtvpqev lqaihivlrh spsinlapvg rsffrppgpn eyndlgggre vwfgyytsvr
     2281 paqwkpmlnv dmsattfyes lplvdfmcrf lsdsrrvltp adfrslrdnq yvrlnrelkg
     2341 lrvkvthlpy prkykvvkit repakeiyfe segsqisvad yfqsryrrls ypnfpcvqsg
     2401 spthpvyipl evcelaegqh crkkldesqt aemikrtakp pakrfqkirq svrdmvsssd
     2461 kylrefgvki nteptqvvgr vldppslvfe nntmckprdg twdlrgqrfy kamsmtkwiv
     2521 lnvsrfahkd slenfirmli rigqelgmri aqplavitfd tnrqpmrtvl teqrkqypql
     2581 emvvavitka tnyaeikqva etelslrtqc ildnnvvqkc naaliqnlcq kinakmggin
     2641 nsllmqekpk lfhrpvivig advshpspgd kvrpsiaacv gsldtvpskf hatirvqied
     2701 seakarveii rdlkdmikel llafhratrh kperivfyrd gavssp
//
LOCUS       XP_077504698             206 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114685 [Amblyomma americanum].
ACCESSION   XP_077504698
VERSION     XP_077504698.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648572.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..206
                     /product="uncharacterized protein LOC144114685"
                     /calculated_mol_wt=21630
     CDS             1..206
                     /gene="LOC144114685"
                     /coded_by="XM_077648572.1:204..824"
                     /db_xref="GeneID:144114685"
ORIGIN      
        1 mlttpaselc issslhfgss ggsvdtspnl wlflqqgrrp qgraaattds grcisstpld
       61 akaralnryp gsettfqrrc qggegvwpaa lggtardrrh cekaehtpsv lcsswpepsy
      121 ttssrfissc tptasvards cslaaaatis alrlhsqqha aflgssttae lmgirlgldl
      181 lltlgppppr sallcdsprr pqppai
//
LOCUS       XP_077504712             748 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077504712
VERSION     XP_077504712.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648586.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..748
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=83949
     Region          83..209
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          300..684
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..748
                     /gene="LOC144114693"
                     /coded_by="XM_077648586.1:28..2274"
                     /db_xref="GeneID:144114693"
ORIGIN      
        1 mrlcshivlv sllcspmlsd aaataksaea aatatqpsvq nlstvqptpe lvmalkglvn
       61 silgspsspl srkliaaevs pdcslglvnf vravrnmepw ayrlidatgk yptglfqgtl
      121 sdvgafdeci etvvrdhygr etvrahycnv yldgnatdvl ryltpaitls hpraidfale
      181 sgnnrfvegv rigicfisdc rdsdiqslle alvegaiqln vkdcvtslnt pmsktqraia
      241 allgvlvati cvssgidiyi srvkanrsff akvvtsfsvr gnleilldva kdpdsdayty
      301 kffhglrffs mfmivighsy nifdfintsr mvnalhyadn isfslimtgy lsvdvfffls
      361 gfllahniep kphnrllmal yaivrrhirt tapvffamms cllvplvvsg pnlkalmekf
      421 yyefrnqwgh illqirnlyc etevgmfghl wfisadfqlf lvalptilil krhywpliav
      481 fisfsllccs vtawqvfntp qppfmtpvva digilldtln dvyvlpfyha mcyfsgcvty
      541 lllkkyrqik iskmkqvgmw cltltcalat vflkydwnrg rgppgqwlki plvfidrimw
      601 sfcvgwtvfa cstnrggvvr rflswsafvp lsrlcfgiyi vhvpfffiks niarerifys
      661 hfslvlqtfg vfvassllsf vmylaceapv grleklllmp thnreearls cpgrqikmlr
      721 hlpctisdaq sanaqdrkes ikgyssrl
//
LOCUS       XP_077504742             390 aa            linear   INV 25-NOV-2025
DEFINITION  DNA-directed RNA polymerase III subunit RPC4-like [Amblyomma
            americanum].
ACCESSION   XP_077504742
VERSION     XP_077504742.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648616.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..390
                     /product="DNA-directed RNA polymerase III subunit
                     RPC4-like"
                     /calculated_mol_wt=41645
     Region          264..376
                     /region_name="RNA_pol_Rpc4"
                     /note="RNA polymerase III RPC4; pfam05132"
                     /db_xref="CDD:461558"
     CDS             1..390
                     /gene="LOC144114711"
                     /coded_by="XM_077648616.1:473..1645"
                     /db_xref="GeneID:144114711"
ORIGIN      
        1 msatpngsps katrtpplpr gvlgrsgtpl lpgtrlpslr gprdltltgn asgvkrtfap
       61 kvdaprktps skddegparg rsseggrgas rhggrgqrrg rgggpsdrgg rfiqtqgatf
      121 sqawkaprll lsqacpelsa igaarwsrse svpvekpkyq apsssssqta eeklqdekkl
      181 evllrsdfid dgpadeswfg pttlplagap vpvpehkpav vvdgqvikre pldkpdvnss
      241 vpctalkkls lvdlctdpni petgellffq lpdslpglqv avqptqkeap ssfvkteeqq
      301 vhngqtklkh fpegyigklq vmksgrvrlv lgsvaltvdm gtnvtfhqel mslrmtegaa
      361 disilgqvmy kliclpemee llkpadsedc
//
LOCUS       XP_077504751             134 aa            linear   INV 25-NOV-2025
DEFINITION  sphingosine kinase 2-like [Amblyomma americanum].
ACCESSION   XP_077504751
VERSION     XP_077504751.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648625.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..134
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..134
                     /product="sphingosine kinase 2-like"
                     /calculated_mol_wt=14808
     Region          <47..115
                     /region_name="YegS_C"
                     /note="YegS C-terminal NAD kinase beta sandwich-like
                     domain; cl29908"
                     /db_xref="CDD:453080"
     CDS             1..134
                     /gene="LOC144114719"
                     /coded_by="XM_077648625.1:1..405"
                     /db_xref="GeneID:144114719"
ORIGIN      
        1 megggeepvl sptdgfpalt dpepddwtve egcfiirysr mvshlfiapd srlddgiawl
       61 llirgdasri qvlsyfkaqe agehvdlpfv rlipmrasrl etftdstitv dgevvktnil
      121 qarelpsmgq iltf
//
LOCUS       XP_077504755             348 aa            linear   INV 25-NOV-2025
DEFINITION  palmitoyltransferase ZDHHC20-B-like [Amblyomma americanum].
ACCESSION   XP_077504755
VERSION     XP_077504755.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648629.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 12% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..348
                     /product="palmitoyltransferase ZDHHC20-B-like"
                     /calculated_mol_wt=39191
     Region          28..297
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; cl19890"
                     /db_xref="CDD:418707"
     CDS             1..348
                     /gene="LOC144114724"
                     /coded_by="XM_077648629.1:1..1047"
                     /db_xref="GeneID:144114724"
ORIGIN      
        1 mvtssggses arrrrsnhef vawlpvamvv glfawayyvy ifvfcgslvk egaqrfafst
       61 vfhlllllcl wsfvqtnvta vppipgyfgf sesdqrlleq caddeareef ldilaenrgv
      121 ltrgpsggvr fcercqqvkp drahhcsqcr rcvpkmdhhc pwfnncvyfs sykfflliif
      181 yvvalcvfgl asathhvagv wssnassyvt lnctflyafg vmlvlvlgsf lylhftmvcn
      241 nvttledlrp hlfkddrdsf digfrdniae vfghrkalwf lpvftslgds arfptrlhpd
      301 ryvygpsvsv vveerrpsvp vpllpvppar sidggpargp hadsfrfp
//
LOCUS       XP_077504764             592 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114732 [Amblyomma americanum].
ACCESSION   XP_077504764
VERSION     XP_077504764.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648638.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..592
                     /product="uncharacterized protein LOC144114732"
                     /calculated_mol_wt=66382
     Region          <42..92
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Region          228..447
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(290..295,336..337,372,374..375,443..444)
                     /site_type="active"
                     /db_xref="CDD:238822"
     Site            order(290..295,336,374)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238822"
     Site            337
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238822"
     Region          465..>534
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     CDS             1..592
                     /gene="LOC144114732"
                     /coded_by="XM_077648638.1:1..1779"
                     /db_xref="GeneID:144114732"
ORIGIN      
        1 mvkvktkkgk tcelevletd vicdqyiptp pvtltdrlri lgyepadftn dggprnigll
       61 igsdhiwelt tgrstkidgk lravetavgw tiqgpvqgid egphcmqtit lrtsvteist
      121 tdiltkfwtl esigindnea ktgknaavef fedtvqlkgg ryevalpwkp vtalednrev
      181 alrrlhqltr rlqnstelln dydaairqys alnmaeivet kdsgantvyh mphqavirgd
      241 hklrvvfdas shnrsaksln dnlesgpnlt adlvgmllnf rrhkialvad ieqaflqisv
      301 rpedrdslrf lwyetkpepg gplppiltwr mkrvpfgtta spfllsatlq hhlknteeqf
      361 pitasrlqrs lyvddilmga eneqaalnly neanaifgaa smqlhkwasn seklcqrfqq
      421 dskgakplgy lsgvlkvlgl twepstdllt fcpctdrist tepptkrsvl qttariydpl
      481 gwlspfivra kvlfqqlwrl niewddalpe giaaewskwl delsylpevk vprysdstqr
      541 kilgdwrkta gkensqgvry lsalprttsq rglratskgq nnrgspvssd ws
//
LOCUS       XP_077504794             125 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114737 [Amblyomma americanum].
ACCESSION   XP_077504794
VERSION     XP_077504794.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648668.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 12% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..125
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..125
                     /product="uncharacterized protein LOC144114737"
                     /calculated_mol_wt=13006
     CDS             1..125
                     /gene="LOC144114737"
                     /coded_by="XM_077648668.1:1..378"
                     /db_xref="GeneID:144114737"
ORIGIN      
        1 mgrassssed ekgalgtkfd eddllrsspa hlssrqsfsg qrardkqpae ggqsavddas
       61 etswleetge tsaellveel qqhrqradta sthqqqptap pssagssrps iaveevagrg
      121 ggraa
//
LOCUS       XP_077504816             129 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114747 [Amblyomma americanum].
ACCESSION   XP_077504816
VERSION     XP_077504816.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648690.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 15% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..129
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..129
                     /product="uncharacterized protein LOC144114747"
                     /calculated_mol_wt=13400
     CDS             1..129
                     /gene="LOC144114747"
                     /coded_by="XM_077648690.1:1..390"
                     /db_xref="GeneID:144114747"
ORIGIN      
        1 masdslagwl qeaaqkgalg tkfdeddllr sspahlssrq sfsgqrardk qpaeggqsaa
       61 ddasetswle etgetsaell veelqqhrqr adtasthqqq ptappssags srpsiaveev
      121 agrgggraa
//
LOCUS       XP_077504848             507 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome P450 3A5-like [Amblyomma americanum].
ACCESSION   XP_077504848
VERSION     XP_077504848.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648722.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..507
                     /product="cytochrome P450 3A5-like"
                     /calculated_mol_wt=57273
     Region          68..490
                     /region_name="CYP3A-like"
                     /note="cytochrome P450 family 3, subfamily A and similar
                     cytochrome P450s; cd11055"
                     /db_xref="CDD:410678"
     Site            order(77,105..108,120..121,211,213..214,216,241,308,
                     311..312,316,380,382,489..490)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410678"
     Site            order(105,119..120,127,131,138,309,312..313,316,381,384,
                     442..445,448..452,456)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410678"
     CDS             1..507
                     /gene="LOC144114767"
                     /coded_by="XM_077648722.1:1..1524"
                     /db_xref="GeneID:144114767"
ORIGIN      
        1 mdvspwaala alaasvafvg vfwvlkrkyd hsffrrhgip gpkpelllgn ykqldkdrik
       61 vmdrwvkqyg kvfgfymaei pyviisdvem ikecfvkhvh afherppmli pvepllssll
      121 yvtgdkwkkv rsvvnpsfss tkmklmtrti vgccettlev isdhvadgkf vnmtklfkgl
      181 sldviskcal awevgcqgdp ndpllkvvrs vaedtedtlv egalkvpafr salrwvfpft
      241 pisriycell erlrqiiylr rsgdkawtld mlqllldaqq gsgrgsidgr klqcplitdd
      301 qllgncfvfl vagyettata msiamyelav hpdeqdaiye elkqalefsg geltyevihq
      361 lkrldmfvce clrmyppial itsrqcnket vvlgqrfpag vnvmaatwqv hhdplvwpdp
      421 fhfdperfrd gvrahhtgaf ipfglgprec lgkrfsalei kaaicttlrk ckvllceetv
      481 vpmkpvvrsv flrsetdiml klglrsp
//
LOCUS       XP_077504887             379 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114796 [Amblyomma americanum].
ACCESSION   XP_077504887
VERSION     XP_077504887.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648761.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 42% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..379
                     /product="uncharacterized protein LOC144114796"
                     /calculated_mol_wt=40156
     Region          284..>340
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     CDS             1..379
                     /gene="LOC144114796"
                     /coded_by="XM_077648761.1:1..1140"
                     /db_xref="GeneID:144114796"
ORIGIN      
        1 mdllkppepl qlsgnlsqnw krfkqklelf ikattpkddp rsgaakasll lsvagdeald
       61 vfntftfgeq edkedydtlv qkfeaycaey vpgkhlvlad mllrsttpgg vdnagatedv
      121 evhalqllng kvtvclgkgm rkdkkkgdsw wessgvfgqr wqpshrscsv alrsaagfvr
      181 astaagprgq paaaadsdsq tpaaersaqp tvahsaepsg srpsygaaaa caanvaassp
      241 rpasaaaavs gsqpaaaalp psppctppva vtehtpeagd ratpgddvki kevgrtvash
      301 fprrpahghl grpiqlianh fnieiptgsv yhyvvgifse taketkpmhq hedqqeshra
      361 pskevparpg klhpgfrrp
//
LOCUS       XP_077504901             826 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114802 [Amblyomma americanum].
ACCESSION   XP_077504901
VERSION     XP_077504901.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648775.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..826
                     /product="uncharacterized protein LOC144114802"
                     /calculated_mol_wt=90159
     Region          <8..128
                     /region_name="EEP"
                     /note="Exonuclease-Endonuclease-Phosphatase (EEP) domain
                     superfamily; cl00490"
                     /db_xref="CDD:469791"
     Site            order(24,54,124)
                     /site_type="other"
                     /note="putative phosphate binding site [ion binding]"
                     /db_xref="CDD:197311"
     Region          193..414
                     /region_name="RT_nLTR_like"
                     /note="RT_nLTR: Non-LTR (long terminal repeat)
                     retrotransposon and non-LTR retrovirus reverse
                     transcriptase (RT). This subfamily contains both non-LTR
                     retrotransposons and non-LTR retrovirus RTs. RTs catalyze
                     the conversion of single-stranded RNA into...; cd01650"
                     /db_xref="CDD:238827"
     Site            order(251..256,310..311,342,344..345)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238827"
     Site            order(251..256,310,344)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238827"
     Site            311
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238827"
     Region          608..722
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     Site            order(611..614,644..646,649,700,718)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260008"
     CDS             1..826
                     /gene="LOC144114802"
                     /coded_by="XM_077648775.1:1..2481"
                     /db_xref="GeneID:144114802"
ORIGIN      
        1 mgpmevsair vrfgdtdtsv asvyvspsvp wdpscltaly srlgkdalic ggfnahhrdw
       61 ggqttttrgr klldavsaag lhllrpraat fihpghstvi dlalhseacr yswkraadsl
      121 gsdhfpifls pgqqqrpkdr eyrlikwpqf reltkeipfa gdfldclksc aqaatvsvsc
      181 haqapvpdlr llacksvefv alqriswiar asdfsecqtg frrhrymads iadvvsclee
      241 akqhrevall dvqaafdslp hstveqalde lgvtgrlrrf vtaflsnrfm qfqvggtlss
      301 prpitagvpq gsvlspflfn lvitglpnai qqdrhrvfcf lyaddialwv raprrhiisa
      361 rqalqralna vksfltskcl vlsakkseal fvhpegarat srirtvqidg tdlrwrrqpl
      421 llhldrvgra aerllargng csanlairlf eaavtpavly vlplvtlktt rlsrleiirr
      481 kwirrllgvp rntqipatla eagvlplnll flqrglthtd rlhhvadgea lfarllgrph
      541 srmgmlsgay leslgrpnsh lalpplptrp ilqvsldfgg tskrhtpaaa irqtaaafle
      601 ecggstllft dgsvltatgr aaaalvapql gvtktcrlsf ppgsstaael aglplaadli
      661 aaipagpvav qalqqrgpts qlrarlhale dagrritlhw vpghsgiegn dladasaksa
      721 hadgspvsla vtqhdfarhl llqvvqtlhp drriaarkpf rrlsdqlrrr drallhrmrs
      781 gctwtpsrly vrgnasspac pscgdtgtlg hlllacpthd lprrsl
//
LOCUS       XP_077504912             273 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114810 [Amblyomma americanum].
ACCESSION   XP_077504912
VERSION     XP_077504912.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648786.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 25% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..273
                     /product="uncharacterized protein LOC144114810"
                     /calculated_mol_wt=29630
     Region          30..55
                     /region_name="ZM"
                     /note="ZASP-like motif; smart00735"
                     /db_xref="CDD:128974"
     Region          96..268
                     /region_name="TBP_eukaryotes"
                     /note="eukaryotic TATA box binding protein (TBP): Present
                     in archaea and eukaryotes, TBPs are transcription factors
                     that recognize promoters and initiate transcription. TBP
                     has been shown to be an essential component of three
                     different transcription initiation...; cd04516"
                     /db_xref="CDD:239952"
     Site            order(100..101,103,130..131,135,137,144,146,150,152,154,
                     156,186,191,193,222..223,228,239,241,243,247)
                     /site_type="other"
                     /note="DNA interaction surface [nucleotide binding]"
                     /db_xref="CDD:239952"
     Site            order(115,122..124,126,131,137,139)
                     /site_type="other"
                     /note="TFIIA interaction surface [polypeptide binding]"
                     /db_xref="CDD:239952"
     Site            order(130,227..228,233,264..265)
                     /site_type="other"
                     /note="NC2 interaction surface [polypeptide binding]"
                     /db_xref="CDD:239952"
     Site            order(203..204,216..220,223,228)
                     /site_type="other"
                     /note="TFIIB interaction surface [polypeptide binding]"
                     /db_xref="CDD:239952"
     CDS             1..273
                     /gene="LOC144114810"
                     /coded_by="XM_077648786.1:1..822"
                     /db_xref="GeneID:144114810"
ORIGIN      
        1 mnspwfglqa svalsggsgg rstpgvdgpk ivhnvynssi hlystqnvae ilaaqagvkl
       61 gqgnlqpraq rpnlmpptsg ppmapitpal adpgivpqik nivstvnlgc rlnlkmlalq
      121 arnvdynpkr cnalimrire prttasifss gkmicagaks eeesrlaark yariiqklgf
      181 daefldfrir nivascdvsf alrleglvlt hspfaryepe lypglifrmv kprivllifs
      241 sgkvvltgak vrseiyeafn niypilksig ink
//
LOCUS       XP_077504936             514 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114823 [Amblyomma americanum].
ACCESSION   XP_077504936
VERSION     XP_077504936.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648810.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..514
                     /product="uncharacterized protein LOC144114823"
                     /calculated_mol_wt=57089
     CDS             1..514
                     /gene="LOC144114823"
                     /coded_by="XM_077648810.1:1..1545"
                     /db_xref="GeneID:144114823"
ORIGIN      
        1 mftaillfqm ltcwlpssac pssdyqpstt afalsgakkw nppwlepppv aansnpariq
       61 hfawqrtasp tspvtrpdra llkqsgcash lgpdtvrctm ftaillfqml tcwlpssacp
      121 ssdyqpstta ftlsgakkwn ppwlepppva ansnpariqh fawqrtaspt spvtrpdral
      181 lkqsgcashl gpdtvrctmf taillfqvgy lpckeyrssn rclvavscpn fvdwrgvmrl
      241 scdmlalgml srlllllsgd velnpgppkk ddkaildalq rleeqhaafs telkqlrttq
      301 itheklissl tnrvlalets qqnlsihtnl netgklkhei avlkaasidt snrmrrnnll
      361 flgledtene swaesetqvl sfcsneldie idptsierah rlgkfnaekk rpiivklahf
      421 ktkdtilacg pklkdtsyai redfafatrl arskliqfir pkkcafklnv dklhvghkcy
      481 vydpvsdsvi esakqpagra qsehlsykps qstd
//
LOCUS       XP_077504948            1162 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114833 [Amblyomma americanum].
ACCESSION   XP_077504948
VERSION     XP_077504948.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648822.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1162
                     /product="uncharacterized protein LOC144114833"
                     /calculated_mol_wt=123934
     Region          7..85
                     /region_name="BRCT_2"
                     /note="BRCT domain, a BRCA1 C-terminus domain; pfam16589"
                     /db_xref="CDD:465186"
     Region          115..183
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cl21498"
                     /db_xref="CDD:473887"
     Region          <178..314
                     /region_name="DMP1"
                     /note="Dentin matrix protein 1 (DMP1); pfam07263"
                     /db_xref="CDD:462128"
     CDS             1..1162
                     /gene="LOC144114833"
                     /coded_by="XM_077648822.1:96..3584"
                     /db_xref="GeneID:144114833"
ORIGIN      
        1 mpkkqrsral fakadgspml fslvpcserk eirelieygg gvlvppekvp dairlvpssv
       61 tvtdssddvf sanyiracak rgkllrlief kipmapavvd asdsktrrlh strsrreytp
      121 geeiaiaefv aknpgvhvhg navykemasa gvlpgkhswq slkehylkni fpvkhlyesp
      181 hevnvlsgkq ssesagdrgs spllaaassv ivedsdseqe arepqrpsgs glqdegapae
      241 vvaetesssp lqssgtspdr aridkesdng relllfasps aksaeksses gldsplakkn
      301 dpdhststsd qgstavalrk hslptpsevl ckkqkelsis tasvcvrsrh slrkastsrv
      361 dvpdtpgqep ilneerglrs slegkcknst prkqriprhm ksgsqisgig rsarttvses
      421 rntlqrtpvk fhtltpvkpn saalqdcsss hlgkqplpas sgssilgdvv kctpcpsrhv
      481 plrislrkrp ntppqeltns hsnsqsspks sgdvavmpkv krgtlspgrf hggdsgameg
      541 lfertpflsr qpvarssprk rqspepqkdc gshsstwrcc esaddtmpdp edllppshar
      601 rrllpcpspa nshkkkvipl rsehrpdsvs steykctdhs htsnssprkq rkireeghnn
      661 vshqnpspss smpthemass atapstrtss giytrscsll qqpslelglr aptakttqgr
      721 kkgprapppp qcgedghsss asqaagtgkn hsaplgpgae vedldsaded llmeallqet
      781 alrnlgpmpg vaevhpvgat keahrddvsl qkpslesglr aptaktargs kkapqapslp
      841 qygedghsss asqgagrgkn qsaplgtgae vedldsaded llmeallqea alrnlgpmpg
      901 vaevhpvgat keahrddvsl qkpslesglr aptaktargs kkapqapslp qygedghsss
      961 asqgagtgkn qsaplgtgae vedldsaded llmeallqea avrnlgpipg vaethpvgat
     1021 keahrdnrsd agspastlsv pstviisdsg hspdedstpe feqdevskvr rrlvrllgml
     1081 regknteshe tcpahcgcpr ttryhrtvre vislsaflay hgeplpsesp giedpeiall
     1141 ieillrdreg rdkneqdgsg sa
//
LOCUS       XP_077504960             988 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114841 [Amblyomma americanum].
ACCESSION   XP_077504960
VERSION     XP_077504960.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648834.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..988
                     /product="uncharacterized protein LOC144114841"
                     /calculated_mol_wt=106483
     Region          7..85
                     /region_name="BRCT_2"
                     /note="BRCT domain, a BRCA1 C-terminus domain; pfam16589"
                     /db_xref="CDD:465186"
     Region          115..183
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cl21498"
                     /db_xref="CDD:473887"
     CDS             1..988
                     /gene="LOC144114841"
                     /coded_by="XM_077648834.1:94..3060"
                     /db_xref="GeneID:144114841"
ORIGIN      
        1 mpkkqrsral fakadgspml fslvpcserk eirelieygg gvlvqpekvp dairlvpssv
       61 tvtdssddvf sanyiracak rdkllrlief kipmapavvd asdsktrrlh strsrreytl
      121 geeiaiaefv akkpgvhvhg navykemasa gvlpgkhswq slkehylkni fpvkhlyesp
      181 hevnvlsgkq ssesagdrgs spllaaassv ivedsdseqe arepqrpsgs glqdegapae
      241 vvaetesssp lqssgtstdr arihkesdng relllfasps aksaeksses gldsplakkn
      301 dpdhststsd qgstavalrk hslptpsevl ckkqkelsis tasvsvrsrh slreastsrv
      361 dvpdtpgqep ilneerglrs slegkcknst prkqpiprym kpgsqisgig rsarttvses
      421 rntlqrtpvk fhtltpvkpn saalqecsss hlgkqplpas sgssilgdvv kctpcpsrhi
      481 plrislrkrp ntppqeltns hsnsqsspks sgdvavmpkv krgtlspgrf hggdsgameg
      541 lfertpflsr qpvarssprk rqspepqkdc gshsstwrcc espddtmpdp edplppshar
      601 rrllpcpspa nshkkkvipl rsehrpdsvs steykctdhs htsnssprkq rkireeghnn
      661 vshqnpspss smpthemass atapstrtss giysrscsll qqpslelglr aptaagtgkn
      721 hsaplgpgae vedldsaded llmeallqet alrnlgpipg vaevhpvgat keahrddyge
      781 dghsssasqg egrlknqsap lgtgaevedl dsadedllme allqeaavrn lgpipgvaev
      841 hpvgatkeah rdnrsdagsp astlsvrstv iisdsghspd edstpefeqd evskvrrrlv
      901 rllgmlregk nteshetcpa hcgcprttry hrtvrevisl saflayhgep lpsespgied
      961 peiallieil lrdregrdkn eqdgsgsa
//
LOCUS       XP_077504973             170 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114847 [Amblyomma americanum].
ACCESSION   XP_077504973
VERSION     XP_077504973.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648847.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 11% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..170
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..170
                     /product="uncharacterized protein LOC144114847"
                     /calculated_mol_wt=18895
     CDS             1..170
                     /gene="LOC144114847"
                     /coded_by="XM_077648847.1:1..513"
                     /db_xref="GeneID:144114847"
ORIGIN      
        1 mnaifasgvr ssdskeqpsp prprpntplf psefqwlasr grpacgerpv cswhppaaah
       61 cthlhrqfra vhcgggssrt rrwtsclgrq gtqqtslrha tgrmpnfqah llehlneaae
      121 drsqltatms dlattvrdar resarlheql vlaisfivvl lqyqlwppst
//
LOCUS       XP_077504977            1081 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114851 [Amblyomma americanum].
ACCESSION   XP_077504977
VERSION     XP_077504977.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648851.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 45% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1081
                     /product="uncharacterized protein LOC144114851"
                     /calculated_mol_wt=106658
     CDS             1..1081
                     /gene="LOC144114851"
                     /coded_by="XM_077648851.1:206..3451"
                     /db_xref="GeneID:144114851"
ORIGIN      
        1 mqalricila alaaatlaas ssikskkihk tprlqrrlgl arielpeprl khlahrllea
       61 krgflsaqvt andghlvgls vkmkhggkpg yhvsvgqgys kkgwhappdl dhvghtslss
      121 gpysggsfqs lfrgseglsf gdasssllse gqpyyykdta slsglypfts yqvpgyvtav
      181 pkyqvtklvd tvpsvlpavs aeydqaslva tplhtstvlq glqlakikal dghasaglgt
      241 klpatrivhk vplsvlpkka afvqqfpatl hgahaklfsp avyaaakvny pamlnngghh
      301 vlslqklpvs klaahlpqsh yvmhtvkpvs saigvirtpi yaatgsgely pvdvdggalq
      361 edyavgadgl qsghanafgg vggqasagyg ngdveglqyg gpqggqlngg gggkygngkg
      421 aaggaengvf qsgqfngggg rkydhgngaa ggadsggfqg gqsngggggk yghgngaagg
      481 fdnggfqggq fnggaggkyg hgngaaggad sggfqggqfn gggggkyghg ngaaggfdng
      541 gfqggqfhgg gggkyghgng aaggadsagf qggqfngggg gkygngngaa ggvdnegfqg
      601 gqfnggggky ghgngaaggv dnggyqeggq lngdggnyah gngaaggfsn ggfngggggk
      661 fahgnsaerg lsnggyqggg ggggggkygf gngaaggign gglqgghfng gggnygqvng
      721 fqqgfgngge gsqevypnkk ghggtdaygn gggpqgfppg lgyqgnsngg glahknygna
      781 gagpqagfan gehqggngft ggaqsgyang gaasngfing gfqgphpggg rpqqhafphg
      841 vtgfnvvpgg lsakhglkga lpaeltyggv pvgglqvqtv qahpfpdipg peesvargqf
      901 ngkargsang raksggvaee gelqggasge gfsavtqvng dyknpqgals gydvkgegaa
      961 ggqhrtlgkq ngeagykngg gwkpivdsry eakfsggsln aeqsseeher entsplvpks
     1021 qpsteaslav kelsasgpse ghrnlrlsqi rcaqkehsaa ngtrksdcpl rpdyngpllt
     1081 n
//
LOCUS       XP_077504985             436 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077504985
VERSION     XP_077504985.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648859.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..436
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=48729
     Region          1..436
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..436
                     /gene="LOC144114856"
                     /coded_by="XM_077648859.1:86..1396"
                     /db_xref="GeneID:144114856"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gtmdavrsgr fgplfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrdcqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqskdssy fvewipnnvk tavcdipprg
      361 lkmsatfign staiqelfkr iseqftamfr rkaflhwytg egmdelefte aesslnelvs
      421 eyqqyqeata edegef
//
LOCUS       XP_077504997             429 aa            linear   INV 25-NOV-2025
DEFINITION  luciferin 4-monooxygenase-like [Amblyomma americanum].
ACCESSION   XP_077504997
VERSION     XP_077504997.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648871.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 11% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..429
                     /product="luciferin 4-monooxygenase-like"
                     /calculated_mol_wt=46584
     Region          <36..410
                     /region_name="AFD_class_I"
                     /note="Adenylate forming domain, Class I superfamily;
                     cl17068"
                     /db_xref="CDD:473059"
     Site            order(76,79..84,86..87)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341237"
     Site            order(79,193..194,216..221,300,312,315,326,410)
                     /site_type="other"
                     /note="AMP binding site [chemical binding]"
                     /db_xref="CDD:341237"
     Site            order(79,121..122,168,170..171,174,193..194,216..221,300,
                     312,315,323..326,391)
                     /site_type="active"
                     /db_xref="CDD:341237"
     Site            order(121,170..171,174,193,323..325,384,391)
                     /site_type="other"
                     /note="CoA binding site [chemical binding]"
                     /db_xref="CDD:341237"
     CDS             1..429
                     /gene="LOC144114867"
                     /coded_by="XM_077648871.1:1..1290"
                     /db_xref="GeneID:144114867"
ORIGIN      
        1 mnqsdasssh vtyeravrsn alhalllwpf llillqnvii fgeaeglvsf sellqsgqtg
       61 efcvprgadp nsmmgafyss gttgvakrva ishrqwiaql lstrelaanq lgpddlvlca
      121 aplshisglw lccgclamgv pllllpsfdq allvpavrkh rvttimiyps ylkslidnap
      181 tdafesvdkv figgstapaa llkeaqvkfs fkfisqgygl tetcgsvtst glkdcgfgsv
      241 gkpvpmvqvk vvdsktgrkl gpgekgeicv kapftfmgyv nkaretaevy ddegfvqtgd
      301 vgfydergdl yvtgrlkeli kcmdlqiepa eierlllqdp avcealvagv pheqfgeavr
      361 afvvlsqgra pdedtrerlr rtvaealpnh ehlhggvefv dhipksetgk siravlrday
      421 lqrqvsirq
//
LOCUS       XP_077505074             329 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114919 [Amblyomma americanum].
ACCESSION   XP_077505074
VERSION     XP_077505074.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648948.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 20% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..329
                     /product="uncharacterized protein LOC144114919"
                     /calculated_mol_wt=36117
     Region          155..>233
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     CDS             1..329
                     /gene="LOC144114919"
                     /coded_by="XM_077648948.1:1..990"
                     /db_xref="GeneID:144114919"
ORIGIN      
        1 mdsvgaaeaa msgrgnqvpa teeyqvilpt lptgrlalnt vflqadtqtr pyrvedfrdm
       61 larqsmltdv lalgayrmsh vwavtfkdae atkkmvslge vkvkqrrcvv idpanqgtri
      121 kvqwllhtvp dedvrvafap ygkvtevske rwrvqeiadn gsttrlvtli lkagtkledl
      181 phqmsiagel alvvvagrqp lclrcrsqgh irrdcrvprc ntcrrfgrte eqcvrtyana
      241 vgvvsgedns elfmdeadae evaktagnna skvvspappl eptteletlv etpraqasga
      301 dnsansketv sdtnnvpvit qsqqhetwk
//
LOCUS       XP_077505144             277 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114950 [Amblyomma americanum].
ACCESSION   XP_077505144
VERSION     XP_077505144.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649018.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 21% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..277
                     /product="uncharacterized protein LOC144114950"
                     /calculated_mol_wt=29980
     Region          <71..105
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..277
                     /gene="LOC144114950"
                     /coded_by="XM_077649018.1:1..834"
                     /db_xref="GeneID:144114950"
ORIGIN      
        1 mggyaspsqf detsdkwpay qirvlaffeg ngltddkknr amlvaaltth tadllsgrca
       61 pdkavpvmaa ariqrwalll saydyvikhq pekddvpada lsrlptsats qpetleetgd
      121 geypgvvipe kllrgyklrs rldnvvappg plarqssvdr pvpgvlrpge pvwvrnfgrg
      181 epwiparits sdgtrmvnad gpeeenirht dqikprvgeh dlhdgagesk rqdgtaspqv
      241 vgtpgqgpgi reaapstatp slrrsartrk ppdryvp
//
LOCUS       XP_077505157             250 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114958 [Amblyomma americanum].
ACCESSION   XP_077505157
VERSION     XP_077505157.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649031.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 35% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..250
                     /product="uncharacterized protein LOC144114958"
                     /calculated_mol_wt=23938
     CDS             1..250
                     /gene="LOC144114958"
                     /coded_by="XM_077649031.1:1..753"
                     /db_xref="GeneID:144114958"
ORIGIN      
        1 mherlnkaak rervwparlf vgvesratlp prilacvlvh ftclalpggy hdygfddhdy
       61 hgygghghdg yykevpgpsf liktvhhtft cvfsalagvt lagvfgggfg gggfggggfg
      121 gghgggitav pvsgvaishg gggfggggfg ggfgggfggg fgggfgggfg ggggkivagp
      181 sylvktihhv nklhgggqll gysggggfgg ggfgggfggg fggggfgggg fggghggtig
      241 iikikgggwh
//
LOCUS       XP_077505180             152 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114970 [Amblyomma americanum].
ACCESSION   XP_077505180
VERSION     XP_077505180.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649054.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 14% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..152
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..152
                     /product="uncharacterized protein LOC144114970"
                     /calculated_mol_wt=14127
     CDS             1..152
                     /gene="LOC144114970"
                     /coded_by="XM_077649054.1:1..459"
                     /db_xref="GeneID:144114970"
ORIGIN      
        1 mktprisvna haeypskfll lfqvlvlfal tavasagllg gggddygggg gfgggggfgg
       61 ggggddidva svsyvktpvv svkyvakpvv syvakpvatv shalkpvlty ttissggggg
      121 ggfggggggg gyggggggyg gggghgggwp ws
//
LOCUS       XP_077505202             152 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114982 [Amblyomma americanum].
ACCESSION   XP_077505202
VERSION     XP_077505202.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649076.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..152
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..152
                     /product="uncharacterized protein LOC144114982"
                     /calculated_mol_wt=17736
     Region          29..>152
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(51..56,93..94,130,132..133)
                     /site_type="active"
                     /db_xref="CDD:238822"
     Site            order(51..56,93,132)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238822"
     Site            94
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238822"
     CDS             1..152
                     /gene="LOC144114982"
                     /coded_by="XM_077649076.1:1..459"
                     /db_xref="GeneID:144114982"
ORIGIN      
        1 mayqdcstsl iinkvvgsri qvkrsvrqdl insllgvlir frkepmaitv dikhmfnwfl
       61 vrddhrnylr flwfrnngiy sdvveyrmkv hvfgnspspa vatyglrrta regeeefgsd
      121 vrqfierdfy vddglnslsk dkdvislirr tq
//
LOCUS       XP_077505220             148 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114994 [Amblyomma americanum].
ACCESSION   XP_077505220
VERSION     XP_077505220.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649094.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="uncharacterized protein LOC144114994"
                     /calculated_mol_wt=12890
     CDS             1..148
                     /gene="LOC144114994"
                     /coded_by="XM_077649094.1:1..447"
                     /db_xref="GeneID:144114994"
ORIGIN      
        1 mrasilvlpa lvavasagii lggggggggf gggggfgggg gfggggggfg ggggggghvi
       61 sssvsyvktp vvsvnyvakp vvsyvakpva tvshalkpvl tytissgggg fgggggfggg
      121 gfggggggfg gggfgggggg ghggwdww
//
LOCUS       XP_077505253             237 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115013 [Amblyomma americanum].
ACCESSION   XP_077505253
VERSION     XP_077505253.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649127.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 34% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..237
                     /product="uncharacterized protein LOC144115013"
                     /calculated_mol_wt=26183
     CDS             1..237
                     /gene="LOC144115013"
                     /coded_by="XM_077649127.1:1..714"
                     /db_xref="GeneID:144115013"
ORIGIN      
        1 mgrsssssed easagvraqg gfcakpprwr nyvleaedrr pppakeqsys rtrsrpspmv
       61 glldmascti igrsmwsssa hlssrqsfsg qrardklpae ggqsaaddas etswleetge
      121 tsaellveel qqhrqradta strqptsssp rhhphrqfqa vhcgggssrt rrwtsclgrq
      181 daqqtslrha tgrmpnfqar lleiwadrir hilcnrsscm qrnvptstvp nlcltpl
//
LOCUS       XP_077505291             115 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115035 [Amblyomma americanum].
ACCESSION   XP_077505291
VERSION     XP_077505291.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649165.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..115
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..115
                     /product="uncharacterized protein LOC144115035"
                     /calculated_mol_wt=12924
     CDS             1..115
                     /gene="LOC144115035"
                     /coded_by="XM_077649165.1:1..348"
                     /db_xref="GeneID:144115035"
ORIGIN      
        1 mkapptrpag flnpfephsr pfqqigmtll gpfpkskrii vatdyltrya einrlterln
       61 ktiadmpamy adaehktwvv iltyvvfayn aavqeettqm tpfrlvygse gtttl
//
LOCUS       XP_077505304            1565 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115047 [Amblyomma americanum].
ACCESSION   XP_077505304
VERSION     XP_077505304.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649178.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1565
                     /product="uncharacterized protein LOC144115047"
                     /calculated_mol_wt=168324
     Region          <105..692
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          <662..1055
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1077..>1473
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..1565
                     /gene="LOC144115047"
                     /coded_by="XM_077649178.1:69..4766"
                     /db_xref="GeneID:144115047"
ORIGIN      
        1 mdfnssdppt rrrrdpfiyr pgpkrgnssd trarhlldpf icrpeseggs srdtprpsvd
       61 ssmsvpgler dnsadistvp rpdpstgtpe sergnrsdia avpqpnpsta tpgpergnss
      121 disagprpds skstpgpeqg nglevpakpr lcmefnssdp ptrrrrdpfi yrpgpkrgns
      181 sdtrarhlld pficrpesee gssrdtprps vdpsmsvpgp erdnsadtsa vprpdpstgt
      241 pesergnrsd iatvpqpnps tatpgsergn ssdipagprp dsskstpgpe qgngleipak
      301 trlcikfnss dpptrrrrdp fiyrpgpkrg nssdtparhl ldpficrpes eggssqdtpk
      361 psvdssmsvp gpernnsadi savprpdpst gtpesergnr sdiaavpqpn pstatpgser
      421 gnssdipagp rpdsskstpg peqgngleip aktrlcmefn ssdpptrrrr dpfiyrpgpk
      481 rgnssdtrar hlldpficrp eseggssrdt prpsvdssms vpglerdnsa distvprpdp
      541 stgtpeserg nrsdiaavpq pnpstatpgp ergnssdipa gprpdsskst pgseqgngle
      601 ipakprlcme fnssdpptrr rrdpfiyrpg pkrgnssdtr arhlldpfic rpeseggssr
      661 dtprpsvdps msvpgperdn sadtsavprp dpstgtpese rgnrsdiatv pqpnpstatp
      721 gpergnssdi pagprpdssk stpgpeqgng leipaktrlc mefnssdppt rrrrdpfiyr
      781 pgpkrgnssd tparhlldpf icrpeseggs sqdtpkpsvd ssmsvpgper nnsadisavp
      841 rpdpstgtpe sergnrsdia avpqpnpsta tpgsergnss dipagprpds skstpgpeqg
      901 ngleipakpr lgtflslpwl eagnssdqps kywmdpfvkh pqsepddspy iparrrldpl
      961 lypplsvqgn spdvssgpmp dpliniaelq lgnssengng ldipprrprl dsfislpgld
     1021 rrnsleapak rrrdpfiyrp gserakssdp sekpplcnse glttscdssa rsrldrftgi
     1081 srrqerssse tptrrrldpf iyrpgserak ssdpsakppl csskapassc dssarsrldr
     1141 vtgisrrqer sssevptrrr ldpfiyrpgs erakssdpta kpplcsskap asscdssars
     1201 rldrvtgisr rqerssseap trrrldpfiy rpgserakss dpsakpplcs teaparrrld
     1261 pfiyrpgser akssdpsekp plcnsegpts scdssarsrl drftgisrrq ersssgtptr
     1321 rrldpfiyrp gserakssds saktplcssk apasscdssa rsrldrvtgi srrqerssse
     1381 aptrrrldpf iyrpgserak ssdpsakppp cpitpvpepe ggkrsgsrsr rrqldpvici
     1441 pgperareap qpdqpaggrr rcasgpaagt rpggdqgedg cyslwprpqa ddpgptfppl
     1501 dpltpqpddy arslfpatpt eeevwphral ffsspsplpd pptadaegss papgkklglq
     1561 vrlat
//
LOCUS       XP_077505321             654 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115060 [Amblyomma americanum].
ACCESSION   XP_077505321
VERSION     XP_077505321.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649195.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..654
                     /product="uncharacterized protein LOC144115060"
                     /calculated_mol_wt=72376
     Region          <3..210
                     /region_name="RT_nLTR_like"
                     /note="RT_nLTR: Non-LTR (long terminal repeat)
                     retrotransposon and non-LTR retrovirus reverse
                     transcriptase (RT). This subfamily contains both non-LTR
                     retrotransposons and non-LTR retrovirus RTs. RTs catalyze
                     the conversion of single-stranded RNA into...; cd01650"
                     /db_xref="CDD:238827"
     Site            order(38..43,98..99,129,131..132,206..207)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238827"
     Site            order(38..43,98,131)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238827"
     Site            99
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238827"
     Region          396..514
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     Site            order(400..401,405..406,431..433,436,459,496,514)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260008"
     CDS             1..654
                     /gene="LOC144115060"
                     /coded_by="XM_077649195.1:1..1965"
                     /db_xref="GeneID:144115060"
ORIGIN      
        1 migfrsglst qdaflllkee vlahvpsgge qhlvmaldlk gafdnvshda ilrelnnlnc
       61 gerifhyvqn flsnrtatig iggtrwdpqd mphkgtpqgs iispllfnia mvgvarslqt
      121 vpnigftlya dditicttkg slatkeetlq qaatcvedta sqcglrcape kseiiriegr
      181 nykspgdiqv llhgvpvret rhirilglwl qsnrkathai kllstitqqv akmirrvarn
      241 rtgmreedtl rlvqalvvnr vsnglpylql trqeltkvna mlrtaykyal gipiytsnae
      301 lealglsnsf eeiqkavlls qrarllrtdt grqtlervgy padvqiiqst tnlpttyrsr
      361 vhispipknm lsgagsgrrs aridylkrta arsphtvyvd aathsevara tavavnsshn
      421 evvsaslkht titgaevaai llaiqygdar nqgvhvmtds lavcrlllag ripkkllgiv
      481 tnsmsahssl rhqitwvpgh aglegnevad alarghtnra gsnpassflp evpftyntrl
      541 hflrtqrqhf ppphkklnaq eawdwrqlqt ytfpnlykys iifleryrge cpwcedrpda
      601 yhitwgckgp kppditthrt keqwetslls selaqqrtlv aqaraaakas gvlk
//
LOCUS       XP_077505349             544 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115080 [Amblyomma americanum].
ACCESSION   XP_077505349
VERSION     XP_077505349.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649223.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..544
                     /product="uncharacterized protein LOC144115080"
                     /calculated_mol_wt=60478
     Region          42..249
                     /region_name="EEP"
                     /note="Exonuclease-Endonuclease-Phosphatase (EEP) domain
                     superfamily; cl00490"
                     /db_xref="CDD:469791"
     Site            order(48,77,143,174,176,226,248..249)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197311"
     Site            order(77,248)
                     /site_type="other"
                     /note="putative metal binding site [ion binding]"
                     /db_xref="CDD:197311"
     Site            order(143,176,249)
                     /site_type="other"
                     /note="putative phosphate binding site [ion binding]"
                     /db_xref="CDD:197311"
     CDS             1..544
                     /gene="LOC144115080"
                     /coded_by="XM_077649223.1:1..1635"
                     /db_xref="GeneID:144115080"
ORIGIN      
        1 maraprsyav apaqsaamrv arhsdtskmg thpkssqnae hieiwqwncr gykrkagllk
       61 qfiattpirp dliclqevga dkaptlagyc tisqplmpsv ailvakdvtv vehalpvpda
      121 ehltveivpn krgrastfvi nvymppkprk gdftdlllaa shkaqcnqfv ivgdfnaphr
      181 ewgycdntar gqsivkaiht lglelltnpv lptregnsvs rdscpdltlv knvshskwtn
      241 lietlgsdhc ilstpipssk irksigpari tdwpkfrnse fptghiesiq dwsaalrqah
      301 statevvqht netpeadrhl lklwdqrakl vrrwktkkln rqlrtrivel teeaqtyatq
      361 lshqnwqqfc eslrgtlgts rtwailrnli dptksksegt rtlkrishlf pgddaallaa
      421 lrdryigsat ttpstityqg spnraldapi ttaevyaaar scvrntaaga dkitnaiirn
      481 lgkdqiqdlt qyindelwem niipqewkhs eiivipkpgk lptlealrsa psrsrrvwas
      541 strg
//
LOCUS       XP_077505362             654 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115084 [Amblyomma americanum].
ACCESSION   XP_077505362
VERSION     XP_077505362.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649236.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..654
                     /product="uncharacterized protein LOC144115084"
                     /calculated_mol_wt=72302
     Region          <3..210
                     /region_name="RT_nLTR_like"
                     /note="RT_nLTR: Non-LTR (long terminal repeat)
                     retrotransposon and non-LTR retrovirus reverse
                     transcriptase (RT). This subfamily contains both non-LTR
                     retrotransposons and non-LTR retrovirus RTs. RTs catalyze
                     the conversion of single-stranded RNA into...; cd01650"
                     /db_xref="CDD:238827"
     Site            order(38..43,98..99,129,131..132,206..207)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238827"
     Site            order(38..43,98,131)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238827"
     Site            99
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238827"
     Region          396..514
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     Site            order(400..401,405..406,431..433,436,459,496,514)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260008"
     CDS             1..654
                     /gene="LOC144115084"
                     /coded_by="XM_077649236.1:1..1965"
                     /db_xref="GeneID:144115084"
ORIGIN      
        1 migfrsglst qdaflllkee vlahvpsgge qhlvmaldlk gafdnvshda ilrelnnlnc
       61 gerifhyvqn flsnrtatig iggtrwdpqd mphkgtpqgs iispllfnia mvgvarslqt
      121 vpnigftlya dditicttkg slatkeetlq qaatcvedta sqcglrcape kseiiriegr
      181 nynspgdiqv llhgvpvret rhirilglwl qsnrkathai kllstitqqv akmirrvarn
      241 rtgmreedtl rlvqalvvnr vsnglpylql trqeltkvna mlrtaykyal gipiytsnae
      301 lealglsnsf eeiqkavlls qrarllrtdt grqtlervgy padvqiiqst tnlpttyrsr
      361 vhispipknm ssgagsgrrs aridylkrta arsphtvyvd aathsevara tavavnsshn
      421 evvsaslkht titgaevaai llaiqygdar nqgvhvmtds lavcrlllag ripkkllgiv
      481 tnsmsahssl rhqitwvpgh aglegnevad alarghtnra gsnpassflp evpityntrl
      541 hflrtqrqhf ppphkklnaq eawdwrqlqt ytfpnlykys iifleryrge cpwcedrpda
      601 yhitwgckgp kppditthrt keqwetslls selaqqrtlv aqaraaakas gvlk
//
LOCUS       XP_077505369             206 aa            linear   INV 25-NOV-2025
DEFINITION  putative L-type amino acid transporter 1-like protein MLAS
            [Amblyomma americanum].
ACCESSION   XP_077505369
VERSION     XP_077505369.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649243.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..206
                     /product="putative L-type amino acid transporter 1-like
                     protein MLAS"
                     /calculated_mol_wt=21957
     Region          36..>172
                     /region_name="2A0308"
                     /note="L-type amino acid transporter; TIGR00911"
                     /db_xref="CDD:273332"
     CDS             1..206
                     /gene="LOC144115088"
                     /coded_by="XM_077649243.1:34..654"
                     /db_xref="GeneID:144115088"
ORIGIN      
        1 maengfqset spllpkgpsi qqdvlpidrt wtstmelkkr lglsngvsmi vgtivgagif
       61 vspagvilys gsiglalvvw vltglaclig alcytelgtm ipksggdyay isaafgpfpa
      121 flqlwtmlvi vkpagnaiva ltfanyvlrp fyddvdchpp ssavmliaal vlckdsgrfc
      181 cvfvsksavl rflpkrsytk dlvqer
//
LOCUS       XP_077505392             249 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115102 [Amblyomma americanum].
ACCESSION   XP_077505392
VERSION     XP_077505392.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649266.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..249
                     /product="uncharacterized protein LOC144115102"
                     /calculated_mol_wt=26828
     CDS             1..249
                     /gene="LOC144115102"
                     /coded_by="XM_077649266.1:1..750"
                     /db_xref="GeneID:144115102"
ORIGIN      
        1 mllsasrcvl lvavataima enatvernig drgrqccpdf nltfpefdee eeqreevrtp
       61 pvqqrgpyyt arpahiltvr qsnlnyllrp yqmpytvvfr rpalrpqrpm altlhpqrts
      121 anrngtlsat pvrvggaigv plysripayg apyyrpyyhh vyrgatlapi paagfvmpyp
      181 appqtglqpp ahvtvpvfin vgtggipaey qpdgdgasgf laavgvppfk gtapqtggfs
      241 akgnasvtv
//
LOCUS       XP_077505399             628 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115110 [Amblyomma americanum].
ACCESSION   XP_077505399
VERSION     XP_077505399.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649273.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..628
                     /product="uncharacterized protein LOC144115110"
                     /calculated_mol_wt=64793
     Region          <13..84
                     /region_name="H15"
                     /note="linker histone 1 and histone 5 domains; the basic
                     subunit of chromatin is the nucleosome, consisting of an
                     octamer of core histones, two full turns of DNA, a linker
                     histone (H1 or H5) and a variable length of linker DNA;
                     H1/H5 are chromatin-associated...; cl00073"
                     /db_xref="CDD:469603"
     Site            38..49
                     /site_type="DNA binding"
                     /note="DNA-binding site [nucleotide binding]"
                     /db_xref="CDD:238028"
     Region          <69..627
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Site            73..84
                     /site_type="active"
                     /note="AKP helix motif (fragment) [active]"
                     /db_xref="CDD:238028"
     CDS             1..628
                     /gene="LOC144115110"
                     /coded_by="XM_077649273.1:1..1887"
                     /db_xref="GeneID:144115110"
ORIGIN      
        1 mrlaaeleva dpkgasaafi rnfifgkypd vygarfktsf kkafisaiek kvlvrtkate
       61 aaegvsgrvk latkakrker aaapaaqpes drsknrrpst ktaskkskss eavategrak
      121 stkarkppgl eekagenakp avadiksknt tgkkaastga eakarkstgv aakdekitga
      181 aakagrstga aaragrstgs takagrspga aakagrstga aakagrstga aakagrssga
      241 atkagksrga atkagksrga atkslkssgt avtvekgnav egkadeenav egkedeenav
      301 egkedegnav evkvgkrrgv pakrvkdkaf ddenkfldle lssengtsva eprsegknsv
      361 aepesdgens saeplsdges ssaepqsede ssaaelqsee entaaepqrd kgfaedavrl
      421 gdignsveen mqgskgenta tekraecgns ssesedtsge sdeplstihm kkepgkkias
      481 kklmglkkta esntarqddf sgtgeldssa ssalkiskls vppqdvgtes sesedtsges
      541 deplntirmk kepgkkiask kltglkktae snsarqddfs gtgepdssas saklsappqd
      601 gptgdsvehd eepkakkqrp kparrakn
//
LOCUS       XP_077505408             205 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115115 [Amblyomma americanum].
ACCESSION   XP_077505408
VERSION     XP_077505408.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649282.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..205
                     /product="uncharacterized protein LOC144115115"
                     /calculated_mol_wt=22967
     Region          18..205
                     /region_name="Cupin_8"
                     /note="Cupin-like domain; pfam13621"
                     /db_xref="CDD:463936"
     CDS             1..205
                     /gene="LOC144115115"
                     /coded_by="XM_077649282.1:1..618"
                     /db_xref="GeneID:144115115"
ORIGIN      
        1 mpashspklv gdsmhaysgv pvvvtdgasn wtalqsfsln ffkdlyqdaa lrmsrsacqf
       61 fpyktefksl eevlnmtaer sklpwyigws ncnpsvanil rthyqapyfl pphsegstld
      121 wifmgspgyg ahlhidhvgk pswqaqirgt klwtlkpvpe cystchelqv vvhpgeifvv
      181 dsnlwyhkte ivgdeisiai gseyd
//
LOCUS       XP_077505432             268 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115129 [Amblyomma americanum].
ACCESSION   XP_077505432
VERSION     XP_077505432.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649306.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 27% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..268
                     /product="uncharacterized protein LOC144115129"
                     /calculated_mol_wt=30634
     Region          187..>248
                     /region_name="DDE_Tnp_1_7"
                     /note="Transposase IS4; pfam13843"
                     /db_xref="CDD:433521"
     CDS             1..268
                     /gene="LOC144115129"
                     /coded_by="XM_077649306.1:1..807"
                     /db_xref="GeneID:144115129"
ORIGIN      
        1 mefddwwqnv ltlhfitgai lirllesess scllsqysra erflsntldn wwrhlctffe
       61 lklltfppkh fenggvqraa fygtcrrgdk prvsdetaea vlqriaqant sdidlsdsdd
      121 dtivdpnfsa pledegtdet sddegpstsg tgraektwkr hqgeittwip dfgdnidsse
      181 qrcswapidy fvqyipnevh ekmalamnrs yvemtgkslq tsaeelktff aisltmsclc
      241 ypqirmywqk kntsttscsr qhvqrsif
//
LOCUS       XP_077505441             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077505441
VERSION     XP_077505441.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649315.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=49516
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144115137"
                     /coded_by="XM_077649315.1:57..1388"
                     /db_xref="GeneID:144115137"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gtmdavrsgp fgplfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkevescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrgcqq yraltvpelt qqmfdvknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkqssh fvewipdnvk tavcdipprg
      361 lkmsatfign staiqelfrr iseqftamfr rkaflhwytg egmdefefte aesnlnelvs
      421 eyqqyqeasd edegefedtp aea
//
LOCUS       XP_077505450             391 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-1 chain-like [Amblyomma americanum].
ACCESSION   XP_077505450
VERSION     XP_077505450.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649324.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..391
                     /product="tubulin beta-1 chain-like"
                     /calculated_mol_wt=43307
     Region          1..390
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..391
                     /gene="LOC144115145"
                     /coded_by="XM_077649324.1:57..1232"
                     /db_xref="GeneID:144115145"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gtmdavrsgp fgplfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tfgdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrgcqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkqssh fvewipdnvk tavcdipprg
      361 lkmsatfign staiqelfrr iseqftgdhr v
//
LOCUS       XP_077505495             713 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 1-like [Amblyomma americanum].
ACCESSION   XP_077505495
VERSION     XP_077505495.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649369.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 30% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..713
                     /product="endothelin-converting enzyme 1-like"
                     /calculated_mol_wt=80907
     Region          105..701
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..713
                     /gene="LOC144115166"
                     /coded_by="XM_077649369.1:1..2142"
                     /db_xref="GeneID:144115166"
ORIGIN      
        1 mpchicdidp plghalpgcv ttpvcaasqv lsqsgqqetp agyctfrtvl lslvsafsfa
       61 ilvaiifill regqpplthk dvvceyrech khaslvgrgl nrsldpcqdf safvcsgwrp
      121 mvdysysvem darirwitsl vaqlsegras glkmmskpea lyrmctlrar tdtakavqel
      181 atfmrelglp wpdrppvpad pmdlmvklsf awdlpswiql sldplrhrpq rvlvrpaahv
      241 ygaerhgqla rlsdpkrfrd ywaafyqtfv agrakideer verfrrmeny vaqafrklla
      301 aetqnpaltt lsamtnesav dwtsalnrhm qpprrftdgd evlmsdrrlr stlsdlltry
      361 rddiqehlgw afvqkyggva neslhavryq anqllsryaa tfacmrdted vfglllaahw
      421 rliiysprer rrldnllrka rehamrelrd apwpmasgtr hetvqrlral svriwpsdrf
      481 hseqalsrvf rrfpstafsy vemwvacrre lsallvgdee vrrelrrlpp agagpllryd
      541 plsnsvsaaa aalgapfyya ygisamwygg lvsvftrelv ramspksava glrtrpsrra
      601 anntdcepgd apwevpnfel paleltfdmw qyqggnaylk dinytaaqif fitvchvmck
      661 kdyqgrkiap gcndavrnfe pfaktfqcea napmnpahkc sffpirnqtp fsq
//
LOCUS       XP_077505518             324 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115173 [Amblyomma americanum].
ACCESSION   XP_077505518
VERSION     XP_077505518.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649392.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..324
                     /product="uncharacterized protein LOC144115173"
                     /calculated_mol_wt=34720
     Region          45..141
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(130..131,133..134)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          162..202
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cl21504"
                     /db_xref="CDD:473889"
     CDS             1..324
                     /gene="LOC144115173"
                     /coded_by="XM_077649392.1:274..1248"
                     /db_xref="GeneID:144115173"
ORIGIN      
        1 mlcsgavrra lcawlalvfa aappvrtkei nefdaktteh qwplgpprev tidaiaehsf
       61 tlrwkpprph pnkhvklqgy mltyaptgka afgeekalml paviqsklvt rlregteyav
      121 vlsavypnvq tasapkltvr talpvaelpv gddsyldnki qcncseegmv actrsalqdv
      181 acscfpsyag rwcescasgy frvgkrcaac pcsnatstge ceaasaegge gvscraclpg
      241 hrgtlcsaca agyhwkgdrc episclsfal carepdnpgc rdchlvqnsl ppvaqkssgt
      301 wpppqtaslr llaiheaacp llrr
//
LOCUS       XP_077505532             390 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115177, partial [Amblyomma
            americanum].
ACCESSION   XP_077505532
VERSION     XP_077505532.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649406.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         <1..390
                     /product="uncharacterized protein LOC144115177"
     Region          <171..234
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     CDS             1..390
                     /gene="LOC144115177"
                     /coded_by="XM_077649406.1:<1..1173"
                     /db_xref="GeneID:144115177"
ORIGIN      
        1 sssergrtdv tsqffdkkkt ifpkyhivhs arkekparli spfivskalt dslgpkykvt
       61 klasgdllle lvgitqiskl adietfgdip vsvtphrsln tlrgiisdnd flhlteeeml
      121 eglseqnvtn vyrikicken keiptkhlvl tfasctlpes iqvgyakisv rpyipnprrg
      181 fkcqrfghgs rscrgrntca kcasndhkse dcdaaprcan cegnhaaysr lcpvwkneke
      241 iitiktkenv tfkearqrya mtrssfsfta htnfadvvrg hgapqrpsap aqltlsevea
      301 gpsapladaa raampppqts pstsksaark aspirekstt pllvgsprns sasteamdkt
      361 rsspqpqrrr gsldhkkekt litgpqqggt
//
LOCUS       XP_077505548            1533 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115179 [Amblyomma americanum].
ACCESSION   XP_077505548
VERSION     XP_077505548.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649422.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1533
                     /product="uncharacterized protein LOC144115179"
                     /calculated_mol_wt=168344
     CDS             1..1533
                     /gene="LOC144115179"
                     /coded_by="XM_077649422.1:618..5219"
                     /db_xref="GeneID:144115179"
ORIGIN      
        1 mlkadkgrrs lprrqppkrv afaddvlvdg asaassdagy gtssadtvvp slrpdaaaks
       61 llslhpdggm ddpyeaseag cgsaarslas gvpgpiffli keqaksllai kelqdrvhal
      121 egfrndiffa vqdiqqtmes ssdrppqvrl lssikepskp vsecrgetra psggpesksw
      181 tvnakprrhk detlmttvcg rarakgagvg sgepgqgvva kpttvaslss kgdtasaile
      241 sdkqdsglds dcrehgsres tlapkdells lldiidersv qlkervrqme katgwdsarq
      301 gsrsevlefh ekelqarlnq ievertkhrm vvrqlqatih rmegekiace eklqaslser
      361 kelekkvhsl hmqyvrggps slplakpeas gawpstslhs lgaqklagec aqgitseegk
      421 akvstilkes splelkklll lytlenqalr ekaneveqhw ftklsewkas easlrtdikn
      481 liqqreeylh alrryqrdma vlqakykele mtvwaldnpe dqrayrrged lppdgnrflc
      541 paepgssrsc ygnvsckdss skaqslppvl lnqakntpll srrgayatef rrnslaensl
      601 eeagfsnedm ayelvdprhq kptafsesqv pqtqktfwqd rrlgaavmps eavrlmnled
      661 dreegaksgp qsniemicne fdplseaerq caskpldfed sldlsiplkp irvsavnask
      721 psrssvnmny ltpasgsski tldqhrtfgk mgaagpvgli fgshtfagar pdvllrhent
      781 fitiliacrr mssffgapav rvaegdrprr grgngrvpra agsrpfagar sfglfavlis
      841 pmypasvksp hrrfswatpa tvcgktqpeg gmdgpyease agcgsaarsl asgvpgpsff
      901 likeqpksll aikelqdrvh alegfrndif avqdiqqtmq sssnqpppvr llssikqpsk
      961 pvsecrgetr apsggaesls wtvnakprrh kggtltttvc grarakyagv gsgepgqgvv
     1021 akpatvasvs skgdtasail etdkqdsgld shcrdhgsre stlapkdell slldiiderc
     1081 vqlkerirqm ekasgwdsaq qgsrsvvlev yekelqarln qievertkhr mvvrqlqati
     1141 hrmegekvac eeklqaslse rkelekkvds lhmqyvsggp sslplakpea aevwpsmslh
     1201 slgaqklage caqgitseeg kakvstifee cnpielkkql llytlenqal rekankdeqh
     1261 wfaklsewka teaslrndiq nliqereeyl halkryqrgk pisqvkykel emtvwaldnp
     1321 edqrayrrge dlppggnrfl rpadpgsmrs cnenfsckds sskaqslppm llnqakntpl
     1381 isqrgayate frrnslaens leeagfsneg madelvdrrl gaavmpseav rlmnleddgd
     1441 kgakpgpqsn iemicnefdp lskaerqcas kpydfedsld lsiplkptrv savnaskpsr
     1501 ssvnmnmnyl ttgsgssrin ldqkhrtfgk nkn
//
LOCUS       XP_077505583             227 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115191 [Amblyomma americanum].
ACCESSION   XP_077505583
VERSION     XP_077505583.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649457.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..227
                     /product="uncharacterized protein LOC144115191"
                     /calculated_mol_wt=19832
     CDS             1..227
                     /gene="LOC144115191"
                     /coded_by="XM_077649457.1:1..684"
                     /db_xref="GeneID:144115191"
ORIGIN      
        1 mrslaslavl aglaatafgg vtvgglgdgl agvgvggsvv linsgrgvgv lkslpgpayv
       61 vksidhlhrv dgggafvtpg gavsfggggg ggggggfagg fgggygggyg ggfgggyggg
      121 ygrglgggyg rgfgggyggg fgggygggfg ggygggfggg ygggfgggyg ggyggyeecf
      181 dggygggygg ggygggfggg ygggfgggfg ggygggyggl ggygykg
//
LOCUS       XP_077505595             131 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115197 [Amblyomma americanum].
ACCESSION   XP_077505595
VERSION     XP_077505595.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649469.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..131
                     /product="uncharacterized protein LOC144115197"
                     /calculated_mol_wt=11657
     CDS             1..131
                     /gene="LOC144115197"
                     /coded_by="XM_077649469.1:1..396"
                     /db_xref="GeneID:144115197"
ORIGIN      
        1 mkayavvlvl sslvaaatag alvagggggy gggyggvgtg lggsvvllsg gygagktvvg
       61 psflvktvhh vnkvsgggav vahsglsgvg ggvgggygdg glayvpvlgl ggyggygggy
      121 ggghgggygv w
//
LOCUS       XP_077505637             322 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115229 [Amblyomma americanum].
ACCESSION   XP_077505637
VERSION     XP_077505637.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649511.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..322
                     /product="uncharacterized protein LOC144115229"
                     /calculated_mol_wt=34906
     CDS             1..322
                     /gene="LOC144115229"
                     /coded_by="XM_077649511.1:1..969"
                     /db_xref="GeneID:144115229"
ORIGIN      
        1 msgppssrsl lrasyppall spntppssps vcfeytslcd lmrssiactp plsrrgseag
       61 spqreplfsr passldngst ewdgdlwsrs tqteaaaens tptdellyer ntgfifrrll
      121 dvqwntsfla svgvlsavvf tvavvatlsa tsnankmads ldlvsdavqr epasvergva
      181 iiapstarqt atvlrrrdag yghisrradr kkarvprrrl rtkakrhrap ppprtssypk
      241 srrqsptlst irrrtaqttd sdssgsfglq qhqlnrrnls pvtigddkvr tprtahngma
      301 sggrkpeapp aspvwdaged vm
//
LOCUS       XP_077505650             387 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115236 [Amblyomma americanum].
ACCESSION   XP_077505650
VERSION     XP_077505650.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649524.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..387
                     /product="uncharacterized protein LOC144115236"
                     /calculated_mol_wt=41653
     CDS             1..387
                     /gene="LOC144115236"
                     /coded_by="XM_077649524.1:1..1164"
                     /db_xref="GeneID:144115236"
ORIGIN      
        1 msetmsrpps srslrrassp pallylktpp pesplrrtts spsicfqyts lcdllrspss
       61 tptpppkepg agsaladper repikvsspa lsrdcnwgcg lwsrstqtep vaesstqtdr
      121 dttdaellqg gqaghtfrrf lgvqwdgsfl mllgvlavvi ftvaviatls gttrankkfd
      181 sldlmpdafe gdpssaqqdv evtapstsre avtvlrssda gkrhsikvhk avskvpkqvp
      241 nvrkavskvs kvpkrpkask vpkavskvrk alskvsevpk vpkattapsr rlktktrkrg
      301 alsaatrpst sphakrwrrr rvlstlrrtr gkvavgrrgl srraqrhvpa rkrstaaisn
      361 dnamaemkag ndvaagseka ssfavda
//
LOCUS       XP_077505662             658 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115241 [Amblyomma americanum].
ACCESSION   XP_077505662
VERSION     XP_077505662.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649536.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..658
                     /product="uncharacterized protein LOC144115241"
                     /calculated_mol_wt=74035
     Region          44..>451
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..658
                     /gene="LOC144115241"
                     /coded_by="XM_077649536.1:125..2101"
                     /db_xref="GeneID:144115241"
ORIGIN      
        1 mcascvaaig lalllmqeeg drlcrsdqcl efagqlnasl dlaadpcdsf ydficgnwns
       61 snafgetlhh rwqhrfrvsv iesnrkqvpt egqtaqqkaa tffqscekiv aqnsseahrl
      121 qqalaeaglf spawtqysel lnatlflntr vrletmvqvs lskgqrtlgd vqdpsgtlll
      181 ymrpsrafle haslrraevr ehfdqvyhyv reylenkeaa aipadelyal hqttrselik
      241 alrqsgqvgv tsnrsevlpf vseaewadll esccglasrh iryrfhrpaf lkeyfalptk
      301 lgpvaaqqyl rwfvmathip vfdaasfvsr ygnsaratae rsrfcfrvvr samgeaftcq
      361 tvlrsfhkdv vanienllpl vyagfvsvfk knawfkpafh vpdyrndsgl vfgileackp
      421 arleetyaey gdmtgsfien wrrvalamla rlkdshvvls aeeheearvh yedkgfaaes
      481 pyyrtrplsa dfellpeasa lpsfgdegpl alrlavagav aadalasllf qeydewdses
      541 rqefavqvrc hlgrritlpe lrpaqrrvmh sflslmalas avqdseeqhh rqgvqglprg
      601 lrgirslfaa wclqhcgrpg greacnrpla emmlfhnaff cgppdtmwkd dacavlfk
//
LOCUS       XP_077505680             391 aa            linear   INV 25-NOV-2025
DEFINITION  glutamine--fructose-6-phosphate aminotransferase [isomerizing]
            2-like [Amblyomma americanum].
ACCESSION   XP_077505680
VERSION     XP_077505680.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649554.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..391
                     /product="glutamine--fructose-6-phosphate aminotransferase
                     [isomerizing] 2-like"
                     /calculated_mol_wt=44067
     Region          <166..390
                     /region_name="PLN02981"
                     /note="glucosamine:fructose-6-phosphate aminotransferase"
                     /db_xref="CDD:215531"
     CDS             1..391
                     /gene="LOC144115253"
                     /coded_by="XM_077649554.1:1..1176"
                     /db_xref="GeneID:144115253"
ORIGIN      
        1 marqfkradp nmteenlrhl mrgvkqelfa glangavidf kdarvtfstk qavarsgsrd
       61 pcsnplhive ndvtvpprci vlvlvwndlf hdyeglvegh aplllelglv varglvrlcd
      121 ghthilltnf sneyrhlikg taiaffhnva evpdsaihrl krtlgenvnr eiitlkmeiq
      181 qiikgnfssf mqkeifeqpe svintmrgrl sfeketvvlg gikdyiaevk rcrrllligc
      241 gtsyhsaiat rqileeltel pvmvelasdf ldrntpvfrd dvcffisqsd qikevlkldd
      301 evkqlaqhly qqksllvmgr gynhatclea hasitegkki kelmymhseg ipagelkhgp
      361 ltlvdkampv mmvltrdpvf pkcmnalqql k
//
LOCUS       XP_077505725             329 aa            linear   INV 25-NOV-2025
DEFINITION  glutamine--fructose-6-phosphate aminotransferase [isomerizing]
            2-like [Amblyomma americanum].
ACCESSION   XP_077505725
VERSION     XP_077505725.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649599.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 31% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..329
                     /product="glutamine--fructose-6-phosphate aminotransferase
                     [isomerizing] 2-like"
                     /calculated_mol_wt=36757
     Region          <108..>324
                     /region_name="PLN02981"
                     /note="glucosamine:fructose-6-phosphate aminotransferase"
                     /db_xref="CDD:215531"
     CDS             1..329
                     /gene="LOC144115277"
                     /coded_by="XM_077649599.1:1..990"
                     /db_xref="GeneID:144115277"
ORIGIN      
        1 mrcicnplhi vendvtvppr csvlvlvwnd lfhdyeglae ghaplllelg lvvarglvrl
       61 cdghthillt nfsneyrhli kgtaiaffhn vaevpdsaih rlkrtlgenv nreiitlkme
      121 iqqiikgnfs sfmqkeifeq pesvintmrg rlsfeketvv lggikdyiae vkrcrrllli
      181 gcgtsyhsai atrqileelt elpvmvelas dfldrntpvf rddvcffisq sgetadtlma
      241 lryckqrgal ivgitntvgs sicreshcgv hinagpeiga astkaytsqf vslvmfslmm
      301 wegrismqpr rteiiqglhr lsgtyiapl
//
LOCUS       XP_077505731             515 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115284 [Amblyomma americanum].
ACCESSION   XP_077505731
VERSION     XP_077505731.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649605.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..515
                     /product="uncharacterized protein LOC144115284"
                     /calculated_mol_wt=57711
     Region          238..436
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(296,335,349,351)
                     /site_type="active"
                     /db_xref="CDD:411747"
     Region          <464..511
                     /region_name="TNG2"
                     /note="Chromatin remodeling protein, contains PhD zinc
                     finger [Chromatin structure and dynamics]; COG5034"
                     /db_xref="CDD:227367"
     CDS             1..515
                     /gene="LOC144115284"
                     /coded_by="XM_077649605.1:152..1699"
                     /db_xref="GeneID:144115284"
ORIGIN      
        1 mkaykalesh nyftsgwvkh lsakefqdgk vvllgevnhs qrlgqkplqv wilckkdgav
       61 itahctcmag ageacshvga clfavetgik mmksrsctqk dnmwlpayve kvqykrlkdi
      121 nftsskgkkr qldsnsfeag pvkerahvpp lseqertkly gsiqdagivp sifsvlpgyd
      181 ghfqnpvpmh darlrnlyse emlahdldvl vkkandvmps iiiseetvka vealtrqqss
      241 ssnwflyrag rvtasvmkrv chtsidnpsi sllkliccpe kqslktpait wgvknepraf
      301 rayqtsetek hdmfkcsksg lhlstrypfv gatpdglvcc accgkgvvel kcpfslrevk
      361 dvtematags cletvngvvq lrrqhgyfyq vqtqmavcdv qwcdfvvwtp nllhveriqk
      421 drhfceeila agrkffihai lpelfstyit rqhagpvvns psdvycfcrg petgkmvacd
      481 naschykwfh fscvalkrap kttlwfcpec krpkg
//
LOCUS       XP_077505765            1107 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115299 [Amblyomma americanum].
ACCESSION   XP_077505765
VERSION     XP_077505765.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649639.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 50% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1107
                     /product="uncharacterized protein LOC144115299"
                     /calculated_mol_wt=119770
     Region          <4..>214
                     /region_name="ASY3-like"
                     /note="Meiosis-specific protein ASY3-like; pfam20435"
                     /db_xref="CDD:466584"
     Region          259..>367
                     /region_name="ArsB_NhaD_permease"
                     /note="Anion permease ArsB/NhaD. These permeases have been
                     shown to translocate sodium, arsenate, antimonite, sulfate
                     and organic anions across biological membranes in all
                     three kingdoms of life. A typical anion permease contains
                     8-13 transmembrane helices...; cl21473"
                     /db_xref="CDD:473875"
     Region          <780..1008
                     /region_name="CitT"
                     /note="Di- and tricarboxylate antiporter [Carbohydrate
                     transport and metabolism]; COG0471"
                     /db_xref="CDD:440239"
     CDS             1..1107
                     /gene="LOC144115299"
                     /coded_by="XM_077649639.1:1..3324"
                     /db_xref="GeneID:144115299"
ORIGIN      
        1 mskkhthnsd grknvrkrhg qdtdsagrss rsgphkryrp ssdeasgtvv gaasqrvsrr
       61 gtrsstprsr hspmptkqik tppnapsaat sstcleqslp rasplqpdsp vpvshdsptp
      121 aassgappsa slgttpadpt anatpalles gspagaspaq ldglcalppv csespevqnr
      181 dlgapppaas nddkkssngq ssvnpawlkr lsllvapkss rgsgpscigy slplylvpfa
      241 lanqemrsvy cilvtvvtla tgflpqpita fvpviliqvt gimapeemaa elmnikvita
      301 cllfmviiig dqtpvfsrla lrvlcnlgfr apllfsytva asfvstfllp sdicviftta
      361 vverfmanle edlsvleqqw srtsrkrtss dtfdqfgesg vrnrskrlrv slgpemvrmt
      421 eamsrksrtt lvgaglgpaq sdwpapsgpl lvkeykihpe pstdakeeaq ttkraprkts
      481 fepfgvvtkw aphlpvrrir sfseqgaanq akrssilkgt ktpespappt dsaqsgpspd
      541 pslldaappe sidvivttsp fsrrpsilkp tggsvqassr rsppspeasp sprmrtasrr
      601 ssilkdvaai rmgskidskr asvlsfeidg qkrssdggif arksgplkmv edystrlfig
      661 pprpayrlpr fiagqqsasf esqpntstds rrvqtmcvsl rtafllgatl tavfgnlasf
      721 wnvaaraail rnlskyatap lvhgvlaasv ygmwrlvceq afifrcrgtr dspeatssye
      781 pitawnwftv tlpvaltgcl vcaaliyvvy lvpfeeldds iqedikkcak arlrelsnrc
      841 vqetimtywl lgfpfyfasn isrsteaayy meapllsisa ivmtmlpaag trplalrrla
      901 swelirsrmp wavvvtygtv ssltklaqer nvvnvlfdwi gprfwerrsa htaqlllcvl
      961 aaflsefign stlndlmvpv vvriasaart hplyfvvpvg viasanvilp mslpllmlre
     1021 sldipctrmi ltglvlktal vlsmlftmnt lgtlifssqe ieqvlrmvsl tpagtnvtrl
     1081 ppqrchsvqs nvtdrnpaet dtdasks
//
LOCUS       XP_077505779             387 aa            linear   INV 25-NOV-2025
DEFINITION  lysosomal aspartic protease-like [Amblyomma americanum].
ACCESSION   XP_077505779
VERSION     XP_077505779.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649653.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..387
                     /product="lysosomal aspartic protease-like"
                     /calculated_mol_wt=42426
     Region          58..371
                     /region_name="Asp"
                     /note="Eukaryotic aspartyl protease; pfam00026"
                     /db_xref="CDD:394983"
     Site            order(76,78,80,118..120,166)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133137"
     Site            76..78
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133137"
     Site            76
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133137"
     Site            order(118..121,125..128)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133137"
     CDS             1..387
                     /gene="LOC144115317"
                     /coded_by="XM_077649653.1:159..1322"
                     /db_xref="GeneID:144115317"
ORIGIN      
        1 mkalvlfsal lsavlavqst natkqlkipl myvgqnrsmn lgrsplterl ssdegmryvg
       61 vvslgtppqs fkvlfdtgsa nlwvpsskcv dsnmgcrars kydsskssty kkngqifkvk
      121 yesgqvrgtl srddlgiagv svrgqlfgei thttgdvfkv vtfdgilglg ypeisvknlv
      181 ppfdnmlkqe lvtdplfsvy lsstpsqedg geilfggvna eryteeitya pvskkgywqf
      241 smdgleigsk tfissptqav vdsgttimag pkevielink ylkatpgprg tyrvkcssvp
      301 kfpkavftig grqfvfegkd ytlqvqtgdk lrcysafedh gesfwvlgqa fmrriytifd
      361 rgndrigfal avqqplhsdc aelrsqn
//
LOCUS       XP_077505829             417 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115349 [Amblyomma americanum].
ACCESSION   XP_077505829
VERSION     XP_077505829.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649703.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..417
                     /product="uncharacterized protein LOC144115349"
                     /calculated_mol_wt=46399
     Region          38..126
                     /region_name="retropepsin_like"
                     /note="Retropepsins; pepsin-like aspartate proteases;
                     cd00303"
                     /db_xref="CDD:133136"
     Site            order(51,53,55,78..80,121)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133136"
     Site            51..53
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133136"
     Site            51
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133136"
     Site            order(78..81,85..88)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133136"
     Region          346..>417
                     /region_name="RT_LTR"
                     /note="Reverse transcriptases (RTs) from retrotransposons
                     and retroviruses which have long terminal repeats (LTRs)
                     in their DNA copies but not in their RNA template. RT
                     catalyzes DNA replication from an RNA template, and is
                     responsible for the replication of...; cd01647"
                     /db_xref="CDD:238825"
     CDS             1..417
                     /gene="LOC144115349"
                     /coded_by="XM_077649703.1:1..1254"
                     /db_xref="GeneID:144115349"
ORIGIN      
        1 mpvhrtnnet tkpeaeqpsq ylpassssel pnikyvktav vnqrsydalv dtgssvctik
       61 asdamgccsc feednlelyg frqspvkcls rfrtaidiyd vkgeyvmlhv vpdtaqhfsv
      121 iightwtelp niayvkvddt fkimekrddt fghlepfsqn qaikittknd valeprsvnf
      181 imssadlege tdvlyqrkgt itgsvfhvas kqlsvpvant sncekvlkkg tslgrveevn
      241 idtaklstts ettevktata dpihekdvat gndvttkerq dllkllnqhr ecfatnieel
      301 gctnliemdi vlkevrrfll dpteqakksv klyrklsasa wkaavivtes rspyaspvll
      361 vckkngeprl vvdyrklntq tvkdkyplpl idgaseqler sklfttldla hgflqvp
//
LOCUS       XP_077505866             163 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115375 [Amblyomma americanum].
ACCESSION   XP_077505866
VERSION     XP_077505866.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649740.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..163
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..163
                     /product="uncharacterized protein LOC144115375"
                     /calculated_mol_wt=17921
     CDS             1..163
                     /gene="LOC144115375"
                     /coded_by="XM_077649740.1:1..492"
                     /db_xref="GeneID:144115375"
ORIGIN      
        1 mtwinqgike mkkysvrcta qcrrqgwqlh kaplperrvt slpfgvappa vraergnrad
       61 heslragrnp gaelhlcrsc qeneassilp tdtqaedest emhatipect sseshqkgdg
      121 iqrlesagts tirlltgstv slkqslqarg thtchqkssr lyt
//
LOCUS       XP_077505877             186 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115379 [Amblyomma americanum].
ACCESSION   XP_077505877
VERSION     XP_077505877.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649751.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 16% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..186
                     /product="uncharacterized protein LOC144115379"
                     /calculated_mol_wt=21398
     Region          80..136
                     /region_name="CBM_14"
                     /note="Chitin binding Peritrophin-A domain; pfam01607"
                     /db_xref="CDD:426342"
     CDS             1..186
                     /gene="LOC144115379"
                     /coded_by="XM_077649751.1:1..561"
                     /db_xref="GeneID:144115379"
ORIGIN      
        1 mtvydrlyma fmevptvyas emnsmpfnsr afdlpvglli lafvsahdva eelrmkrqty
       61 alphgselll eplrtrficr hdgyfadvdn ncrvyhictr ssetrqlqrf sflcgnltmf
      121 nqltltcarp eesvpcrnap vfyyvndnig yqdtpflydd dvsnadqfih nnrllqaana
      181 lpkqpf
//
LOCUS       XP_077505920             276 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115407, partial [Amblyomma
            americanum].
ACCESSION   XP_077505920
VERSION     XP_077505920.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649794.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: incomplete on the carboxy end.
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..>276
                     /product="uncharacterized protein LOC144115407"
     CDS             1..276
                     /gene="LOC144115407"
                     /coded_by="XM_077649794.1:1..>828"
                     /db_xref="GeneID:144115407"
ORIGIN      
        1 maasslpfec eldsdncven vscfrvalnn dgdayewlts ygvatrttwi vdwevakpkr
       61 iafhkkwkcq hsnrnkitgq tatncpafvd ikiknitrds rkrdpflkra tplraivkvr
      121 ddhnhaldca dalrllrtta dtravfhsyf hdgltpaqai amhqqkleae ddaaeklasg
      181 avnpsagtvy ywyrlwrdhh ygsavdpvsk lsekapcyld kgadvrttrs edgtcwavlv
      241 vtqvmkrtqg lsaakeiifv dstascdesq ssltvv
//
LOCUS       XP_077505965             115 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115436 [Amblyomma americanum].
ACCESSION   XP_077505965
VERSION     XP_077505965.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649839.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 33% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..115
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..115
                     /product="uncharacterized protein LOC144115436"
                     /calculated_mol_wt=11829
     CDS             1..115
                     /gene="LOC144115436"
                     /coded_by="XM_077649839.1:38..385"
                     /db_xref="GeneID:144115436"
ORIGIN      
        1 mtrvgnqtds dggidlppva slpvirllqp mvdgagneef hyaeddwepp lvdepqspaa
       61 avgksgatpe aieqesnfff pavqpadass taaagcstrl ggnrhtapsp pcmqv
//
LOCUS       XP_077505984             274 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115457 [Amblyomma americanum].
ACCESSION   XP_077505984
VERSION     XP_077505984.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649858.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 31% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..274
                     /product="uncharacterized protein LOC144115457"
                     /calculated_mol_wt=28992
     CDS             1..274
                     /gene="LOC144115457"
                     /coded_by="XM_077649858.1:11..835"
                     /db_xref="GeneID:144115457"
ORIGIN      
        1 meaatpagan ctagcpassa stagdledve eatsdptmlk vlrllleirq qqreilqrls
       61 rleqarqepr trspsppvss lpplcpqlpa ftiedfeaae aavrddrvaa alkkqllrlg
      121 gnslkevaan vmgavmgvav qrlfslrgrk gkrpfvgtkl ctvatdaice rqgvdilaaq
      181 gfigrwlpga cdrgggrkrr yaqafeppes haqappvnpc aggppklcpp qppphaqpps
      241 tqalplplpp hsllltpshp tqhpchmghc ptgt
//
LOCUS       XP_077505993             389 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115466 [Amblyomma americanum].
ACCESSION   XP_077505993
VERSION     XP_077505993.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649867.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 37% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..389
                     /product="uncharacterized protein LOC144115466"
                     /calculated_mol_wt=43674
     Region          <165..236
                     /region_name="MULE"
                     /note="MULE transposase domain; pfam10551"
                     /db_xref="CDD:463145"
     CDS             1..389
                     /gene="LOC144115466"
                     /coded_by="XM_077649867.1:1..1170"
                     /db_xref="GeneID:144115466"
ORIGIN      
        1 mefhkvwrcq hshrnkpvsq rnlgcpakld ikikkvnqnt krndpflrre tplpavikls
       61 yrhnhstesa dalrllhpsp etkaeflayf eagmspcdai rhqewidvht ttkddywavv
      121 iatpimrriq amgsaqniff vdstascdtt kttitvllap tkagavpiav llhsaqnteg
      181 ysqafsllkq hypscfgnrk apavfmtdns rpekdalqat wpsahqllch fhvlqaewrw
      241 ltstpsnvpk dkrqqfmaaf qkvwkfctis kdelkaakea lvttchegyi krvfkvldqe
      301 ehcwagsqga fckhqavvqk afgglfpnsp qlttadcill aqlalgdrcp pqaffaplqt
      361 aqqvempppp lnaaddhnys qknpsvhiq
//
LOCUS       XP_077506052             440 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115514 [Amblyomma americanum].
ACCESSION   XP_077506052
VERSION     XP_077506052.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649926.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..440
                     /product="uncharacterized protein LOC144115514"
                     /calculated_mol_wt=47243
     Region          45..433
                     /region_name="MenE/FadK"
                     /note="O-succinylbenzoic acid-CoA ligase MenE or related
                     acyl-CoA synthetase (AMP-forming) [Lipid transport and
                     metabolism]; COG0318"
                     /db_xref="CDD:440087"
     CDS             1..440
                     /gene="LOC144115514"
                     /coded_by="XM_077649926.1:1..1323"
                     /db_xref="GeneID:144115514"
ORIGIN      
        1 mafedclpkg lhvpkkasvt vkidngvvaa haqgvtvpdv dfstffrdvf gtykdetavv
       61 dartgaqytf gelydaslrv aaglrrlglr pgdvvgfqga ngaqlvvams gtffaggtaa
      121 laktkltern ihyqfadaqp kfvvcdlgda rdmlkaceki tsveatsmll fpmfvrklnv
      181 hpllgktdts tlkqlivggn tvsspvlqsv aeklrlkgvi qaygmteltg cvtfsiprhd
      241 dfksvgqpva faemkvvdld trktlgplek geicvrgact fkgylgrped tarawengfi
      301 rtgdmgfyap dgrffvcgri kelikcmdqp vapaeleell atdpgvrhvv vagvphpefg
      361 eaarafvvle lqggvaegae aaarlkalva gqlslhkhlh ggveflesip htdsgkdlrr
      421 alqqsylqkh kgaskhaqad
//
LOCUS       XP_077506061             411 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115521 [Amblyomma americanum].
ACCESSION   XP_077506061
VERSION     XP_077506061.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649935.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 25% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..411
                     /product="uncharacterized protein LOC144115521"
                     /calculated_mol_wt=42826
     CDS             1..411
                     /gene="LOC144115521"
                     /coded_by="XM_077649935.1:1..1236"
                     /db_xref="GeneID:144115521"
ORIGIN      
        1 mrdfeeivcf agqsiadeac pvvrrkpsgs gsslvesggd dlhttelrng cqagaattvt
       61 maqpdeswtt pagpfvvmap prdpgtfsgt dvddwlnlsk lntrfillla aacagayevs
      121 narrgslvgl rsnafdfsps gtsqkvvftg pnalermlsq sighsleqgl vsgtggqrai
      181 vqeigtgfep ggghiightg vhglqqvfaa pamgqgvaaf gglqglgqgf grnvgkglqh
      241 aivpsftpql tqlsqlphma qlpqqggsrg lsqgvaiaaa rgvghtfghg lthakasapq
      301 rsvgrqvlha vapagsyasa sgfgssqglv rafqpdygss faspyrssvh ddfafgeskv
      361 ihgptylvht fgterdpyyg srksysgksh rgskpyvrks imikdkgrhr p
//
LOCUS       XP_077506071             492 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115528 [Amblyomma americanum].
ACCESSION   XP_077506071
VERSION     XP_077506071.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649945.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..492
                     /product="uncharacterized protein LOC144115528"
                     /calculated_mol_wt=40945
     CDS             1..492
                     /gene="LOC144115528"
                     /coded_by="XM_077649945.1:62..1540"
                     /db_xref="GeneID:144115528"
ORIGIN      
        1 mrsliviasl igltvagslg gyggpssgda agdsggggsg wqpeaggggk ggssgggwqp
       61 qggdsgaggg gggsggwqas ggtggggggg gsgwqpqggg gsggagwqpa ggggaggwqg
      121 gglgaaagag agsghggsgg dgvkhiyiir tvtttggggg ggggghgggg hggagksghg
      181 ggavggwqag ggsggsgwqa gggaggwqag ggggasgwqv ggstggwpsg ggggagsggw
      241 psggggsgag gwpsgggsag wqsggsggwq tggggghggk ggggaaggat khvyiiktls
      301 sssgggshgg tgghgghsgg gahgggghgg tsghggagah ggagghgghg ggqhgasggg
      361 apesiivirt dgggaggghg ghggghgghg gghggvssfg gaaavshgtv asaaagavwr
      421 atlggggaga gwpaavsdga gagwqsggss ggwqsggggg sgggwqpsgg dvgggwqpag
      481 gaagggggag ww
//
LOCUS       XP_077506092             276 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115536 [Amblyomma americanum].
ACCESSION   XP_077506092
VERSION     XP_077506092.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649966.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 36% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..276
                     /product="uncharacterized protein LOC144115536"
                     /calculated_mol_wt=30764
     Region          <146..209
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     CDS             1..276
                     /gene="LOC144115536"
                     /coded_by="XM_077649966.1:1..831"
                     /db_xref="GeneID:144115536"
ORIGIN      
        1 mstasapgpd giptsfyarf ytvleehllr mvkdfvrdgv ssqhgdedgi vqveattepa
       61 etttkpwqtt tsmltttlgs laefcpdsgn idvyverfdl ystaketdas kklqvfltil
      121 gekayvtlrs lllpkkptev khekatealr khyapkrsvv tapyhfyqrk qepneslqqf
      181 ivelkrlaat csfgsfleea lrdrliagig tdsircrlla lsddevtwer vckiatalet
      241 aqkdtreilp vlrppdtrrl tlrntsavat nfgaim
//
LOCUS       XP_077506106             576 aa            linear   INV 25-NOV-2025
DEFINITION  luciferin 4-monooxygenase-like [Amblyomma americanum].
ACCESSION   XP_077506106
VERSION     XP_077506106.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649980.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 12% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..576
                     /product="luciferin 4-monooxygenase-like"
                     /calculated_mol_wt=62497
     Region          73..555
                     /region_name="AFD_class_I"
                     /note="Adenylate forming domain, Class I superfamily;
                     cl17068"
                     /db_xref="CDD:473059"
     Site            order(218,221..226,228..229)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341237"
     Site            order(221,337..338,360..365,443,455,458,469,555)
                     /site_type="other"
                     /note="AMP binding site [chemical binding]"
                     /db_xref="CDD:341237"
     Site            order(221,263..264,310,312..313,316,337..338,360..365,443,
                     455,458,466..469,536)
                     /site_type="active"
                     /db_xref="CDD:341237"
     Site            order(263,312..313,316,337,466..468,529,536)
                     /site_type="other"
                     /note="CoA binding site [chemical binding]"
                     /db_xref="CDD:341237"
     CDS             1..576
                     /gene="LOC144115553"
                     /coded_by="XM_077649980.1:1..1731"
                     /db_xref="GeneID:144115553"
ORIGIN      
        1 miytseikln vqkldlaplr qlpseapcel gkiccmmdkr ppsyyaqrpl iqemlslsnl
       61 yaqsndarvp fsqvdartga qytfgelyda slrvaaglcr lglrpgdvvg fqgangaqlv
      121 vamcgtffag gtaalaktkl tereiyyqfa dtkpkfvvcd lgdarnmlka cekitsveav
      181 ivttgsyega lsvselmkth vedsdtpvkl spdavlaviy ssgstglpkg vqlthrniig
      241 qvvsygclda svfkkgdifl csaslmhvgg fwltwcylgh gckvvhvhts dfsvvlpaie
      301 kykattmlly ptfvhklkvh plldqsdtst fkklivagnt vsspvlqsva rklrlkgviq
      361 aygmteltgc vtfsiprhdd fksvgqplaf aemkvvdpdt rkalgplekg eicvrgastf
      421 kgylgrpedt arawedgfir tgdvgfyapd grffvcgrik elvkcmdqqv apaeleella
      481 tdpgvrhvvv agvphpefge aarafvvler hggvaeeaev aarlkalvag qlslhkhlhg
      541 gveflesiph tdsgkdlrra lqqsylqkhk gaskha
//
LOCUS       XP_077506151             178 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115600 [Amblyomma americanum].
ACCESSION   XP_077506151
VERSION     XP_077506151.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650025.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 23% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..178
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..178
                     /product="uncharacterized protein LOC144115600"
                     /calculated_mol_wt=18845
     CDS             1..178
                     /gene="LOC144115600"
                     /coded_by="XM_077650025.1:228..764"
                     /db_xref="GeneID:144115600"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrppfa altsvlccqr pasigpcvlp afgcvippdp
       61 fmlktkscme vvqatklrvi fardleqagk lvdgqwysls kasffsktkt iflgswamyl
      121 gtcsqdnagq hasasskkkh etlsdgvvgg aalvlnqnge vvcigselgl fnlaettg
//
LOCUS       XP_077506160             226 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115610 [Amblyomma americanum].
ACCESSION   XP_077506160
VERSION     XP_077506160.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650034.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 41% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..226
                     /product="uncharacterized protein LOC144115610"
                     /calculated_mol_wt=25927
     Region          <1..46
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..226
                     /gene="LOC144115610"
                     /coded_by="XM_077650034.1:67..747"
                     /db_xref="GeneID:144115610"
ORIGIN      
        1 manlgyhwtw qqlrncwknl kkrftaerle qersgaapsa wkwydhmsal lshrpmvqar
       61 eygvdsqdvp pdygdnsqld isdadtrtee ddadilqeqs epstssrspp qkrrrmgeak
      121 lekvllnvqr knefwtkqae iqfqqqkqll adenkhmqel lqgvsttflq gtqtlmgqff
      181 sqqaalvasl qhmpsfpqfm gppnmnlqhq fpppppatnt pptpkh
//
LOCUS       XP_077506189             233 aa            linear   INV 25-NOV-2025
DEFINITION  paired mesoderm homeobox protein 2-like [Amblyomma americanum].
ACCESSION   XP_077506189
VERSION     XP_077506189.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650063.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..233
                     /product="paired mesoderm homeobox protein 2-like"
                     /calculated_mol_wt=24633
     Region          128..184
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..233
                     /gene="LOC144115635"
                     /coded_by="XM_077650063.1:1..702"
                     /db_xref="GeneID:144115635"
ORIGIN      
        1 mrpsprsgrr aggtgpssla arrkragppr rwaaagaqsa smgpenqaaa qgfsvqrlld
       61 qapaaqrarq pstgatasrs alpprgpwee tfgerlapia spaatagrgs ssggggnesv
      121 dgraeaarrs rtaftaqqls alekafersh ypdafareel aatvnlsear vqvwfqnrra
      181 kfrrnersaq rvargeaseq pvmaapphat wphcaaapll phppalhspa rwc
//
LOCUS       XP_077506202             772 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077506202
VERSION     XP_077506202.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650076.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..772
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=86620
     Region          109..237
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          333..724
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; cl46765"
                     /db_xref="CDD:481105"
     CDS             1..772
                     /gene="LOC144115641"
                     /coded_by="XM_077650076.1:19..2337"
                     /db_xref="GeneID:144115641"
ORIGIN      
        1 mrlllvllaf vpllcaedgl teaveggstt ltpmttettt etestttewt testtestte
       61 ttalgftdtp esttldfeea idgfvkdisk rmgpfaslil sdenitedcs gallkvmlgl
      121 rskspwallm ldanarpasg tlngatgsyg dyeecldvhh qpsngdaaiy gqyctlfmtl
      181 plgfarksaq rmqkdgyflg rrspdffssd arsyfkgqrl glclpqlcti ddvrrmislt
      241 ldpygfkhhv sgcdsilnee rgwnpirigm vslvsvlafl lvvssgldlf lkkdsrarse
      301 dspwkylmaf satintrkll ncnfkegsdn qilasfagik vlsttwvilg htyfivdisa
      361 vrsyfkvida teelfftlig nafasiatff figgfllsfn vlkrmknlkg nylivlwiml
      421 irryirltvp vmfmvgwgyv msmcvrgpaf fevlgqndln cnnswwmvll hvnnwqsfln
      481 mvsigtgkla nrasarilai nhrvdaftdp rklrvlpvms trcmerrwar ekrdnqnkhn
      541 lgavclcrrp sdtvdyvyvr pfthvgsyct gilfgyitlk yghvkiskmv qsalwmasta
      601 valcvvmvpy ewqkgnfpsq lesgfyaglh rtawavaigw lvfacvtgra gpvnrflsws
      661 ffvplsrlsl gayltsvfil airvtyrrei aeyhhfpmvm lflgatmlsy maayvlflfv
      721 ecpiglieky lfmskavver hdpaahtnga fngsvekite aekekrfvve rf
//
LOCUS       XP_077506217             250 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115649 [Amblyomma americanum].
ACCESSION   XP_077506217
VERSION     XP_077506217.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650091.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 21% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..250
                     /product="uncharacterized protein LOC144115649"
                     /calculated_mol_wt=26834
     CDS             1..250
                     /gene="LOC144115649"
                     /coded_by="XM_077650091.1:195..947"
                     /db_xref="GeneID:144115649"
ORIGIN      
        1 mkdvrplepg gsaaaaqrkr fhqsseassq atvlhsatse dslagdgfvl vmskkakrrl
       61 rksedsssss tsavittrga pahtvlftpe tpidnfnlln rqatsvflea rvpgvikdvr
      121 iksrrnllai dvtersales latikkigdi pvrslvledn gttaggiydi danipdsdlp
      181 ilikpatnsi eitkvqrlgk sssthqpppp argyppppwv hlpipplptf tlqkfhppqv
      241 llleahtaae
//
LOCUS       XP_077506229             198 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115654 [Amblyomma americanum].
ACCESSION   XP_077506229
VERSION     XP_077506229.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650103.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 38% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..198
                     /product="uncharacterized protein LOC144115654"
                     /calculated_mol_wt=21850
     CDS             1..198
                     /gene="LOC144115654"
                     /coded_by="XM_077650103.1:51..647"
                     /db_xref="GeneID:144115654"
ORIGIN      
        1 msypgaypys gdyatvlqpr yqhpmmmdpr mmgmygptmp yldgpqhsdn htqnllfyic
       61 slsfmclfvv vmvysaiemr rrihelatda pnrtayatvs sstaettvrp frwdtgidkl
      121 lddsdddrlv pdhkgraggg ggdttstpdf gcgavvrlgw ppspessves marvqvnvng
      181 ylsrqwifae lgcsslra
//
LOCUS       XP_077506242             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115660 [Amblyomma americanum].
ACCESSION   XP_077506242
VERSION     XP_077506242.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650116.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 33% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115660"
                     /calculated_mol_wt=20779
     CDS             1..181
                     /gene="LOC144115660"
                     /coded_by="XM_077650116.1:1..546"
                     /db_xref="GeneID:144115660"
ORIGIN      
        1 mrepisdpet vfgkparavi llerrhfets qivrnensfk psprwrpsav csvrgmpvwp
       61 rghsgtpgyr elqskvaele aklrerqdgs qqtspstpmr vhsldrsnhe mdclrndsvq
      121 prgqwksllp kwhgmmktwt vycihdgtrt cvqcfrqlmd ptamcrcaic vvchenktyv
      181 q
//
LOCUS       XP_077506265             123 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115675 [Amblyomma americanum].
ACCESSION   XP_077506265
VERSION     XP_077506265.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650139.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..123
                     /product="uncharacterized protein LOC144115675"
                     /calculated_mol_wt=11469
     CDS             1..123
                     /gene="LOC144115675"
                     /coded_by="XM_077650139.1:221..592"
                     /db_xref="GeneID:144115675"
ORIGIN      
        1 mntlaifasi lllaaavnag fvggyggygg ygggygggyg ggygyggygr glgyggyggy
       61 ggfggygggf gkiigigygg yggyggyggy ggyggyggyg gyggyggygg yggyggyggy
      121 gky
//
LOCUS       XP_077506277             332 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115680 [Amblyomma americanum].
ACCESSION   XP_077506277
VERSION     XP_077506277.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650151.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 37% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..332
                     /product="uncharacterized protein LOC144115680"
                     /calculated_mol_wt=36914
     Region          219..243
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          253..273
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(258,260,262,264..265,268..269,272,284,286,290..291,
                     294..295,298,312,314,316,318..319,322..323,326)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          279..299
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          307..326
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..332
                     /gene="LOC144115680"
                     /coded_by="XM_077650151.1:1..999"
                     /db_xref="GeneID:144115680"
ORIGIN      
        1 mvrvwrgrkk kttsrnadad aaqqrvagrs ahaqsehapr aalkqakqpr wrpqhtprig
       61 kediivvlkp retidlkatl gpgqasaair silgdaphrp ssnergdtki apagsarvps
      121 raplnrgtsl adrcglpaas lsplllrrpv lreepgatfp qttaslltvr fpaltemeas
      181 pgspkermgs svspevshvs nrtrrlyiql gtentihvcw pckvsfklps kfiahrlykh
      241 gihgtprrhk vecpichrky knqtimtqhm gfhmgerqcf mcgaqfaslr saiihkrlhr
      301 nggrfqcgfc reafahkgdq eehlrldhaq qq
//
LOCUS       XP_077506289             247 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115689 [Amblyomma americanum].
ACCESSION   XP_077506289
VERSION     XP_077506289.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650163.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..247
                     /product="uncharacterized protein LOC144115689"
                     /calculated_mol_wt=27175
     CDS             1..247
                     /gene="LOC144115689"
                     /coded_by="XM_077650163.1:1..744"
                     /db_xref="GeneID:144115689"
ORIGIN      
        1 measdvrkka epqryrrehs nlfvtaagav vtlfctavfr clrrsaskka ftarcssagc
       61 eaqllgddvm spskdihdyk ctskrsghhr fgrafwehav gaaeslvrsa dgscrialpa
      121 allykpcqdv afrkkvetep lrgplgdvgp qercsswvfw csimlmpsmt nsdatsqdhs
      181 sfdealeahq dsysrdvdgf sllrrsvlll aqfspafhcr rghpmvafla craipgeqnl
      241 lvaavce
//
LOCUS       XP_077506297             194 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115696 [Amblyomma americanum].
ACCESSION   XP_077506297
VERSION     XP_077506297.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650171.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 41% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..194
                     /product="uncharacterized protein LOC144115696"
                     /calculated_mol_wt=21406
     CDS             1..194
                     /gene="LOC144115696"
                     /coded_by="XM_077650171.1:1..585"
                     /db_xref="GeneID:144115696"
ORIGIN      
        1 measdvckkd ehqryrrqhs nlfatatgav vtlfctavfr rsaskeplap rcssdgceah
       61 llgddvmspt kdihdykcts krsghhrfgr afwehavgaa eslvrsadgs crialpaall
      121 ykpcqdvafr kkveteplrg plgdvgpqer csswvfwcsi mlmpsmtnsd atsqdhssfd
      181 ealeahqhsy srdg
//
LOCUS       XP_077506305             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115705 [Amblyomma americanum].
ACCESSION   XP_077506305
VERSION     XP_077506305.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650179.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 18% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115705"
                     /calculated_mol_wt=20042
     CDS             1..181
                     /gene="LOC144115705"
                     /coded_by="XM_077650179.1:1..546"
                     /db_xref="GeneID:144115705"
ORIGIN      
        1 mrdvpqrhra laqiqtrehf rmrsppgmmf fssrrvlrsl rpleiqalpp lesagfagkv
       61 qgphqpeeva qssdegpfgd dcrfqliasv salmgrtdla shaeifkgsa lfmnhwpgay
      121 anvtstewkr ltmlleangs cshrtlrelp dpavacgrav pctawdynll akqailqens
      181 q
//
LOCUS       XP_077506318             201 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115713 [Amblyomma americanum].
ACCESSION   XP_077506318
VERSION     XP_077506318.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650192.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 16% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..201
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..201
                     /product="uncharacterized protein LOC144115713"
                     /calculated_mol_wt=22361
     CDS             1..201
                     /gene="LOC144115713"
                     /coded_by="XM_077650192.1:1..606"
                     /db_xref="GeneID:144115713"
ORIGIN      
        1 mrdvaqrhra ltqiqtrehf rmrsppammf fssrrvlrsl rpletqalpp lesagfagkv
       61 qgphqpeeva qssdegpfgd dcrfqlvapv salmgrtdla shaeifkgsa lfmnhwpgay
      121 anvtstewkr ltmlleadgs cshrtlrelp dpavacgrav pctawdynll pkqailqgsa
      181 lfmdhwpaay anmnaaewkr q
//
LOCUS       XP_077506328             229 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115721 [Amblyomma americanum].
ACCESSION   XP_077506328
VERSION     XP_077506328.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650202.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 41% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..229
                     /product="uncharacterized protein LOC144115721"
                     /calculated_mol_wt=25031
     CDS             1..229
                     /gene="LOC144115721"
                     /coded_by="XM_077650202.1:1..690"
                     /db_xref="GeneID:144115721"
ORIGIN      
        1 mffssrrvlr slrpletqaq ptwqsasfar kgqgprqaed vaqssdegpf gddgrfqlva
       61 lasalmartd fashaesfkg salfmdhwpa ayanmnstec kwlavllegd dscshrtlre
      121 lpapayscgr ilpcttwdyn llpkqailqc salvmdhwpa ayanmnaaew krpvqqlvgd
      181 csdspcvplk ptvpavpygr avtstdpdhn palrgssavs ewslvcdrl
//
LOCUS       XP_077506341             440 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115727 [Amblyomma americanum].
ACCESSION   XP_077506341
VERSION     XP_077506341.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650215.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 39% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..440
                     /product="uncharacterized protein LOC144115727"
                     /calculated_mol_wt=48392
     CDS             1..440
                     /gene="LOC144115727"
                     /coded_by="XM_077650215.1:1..1323"
                     /db_xref="GeneID:144115727"
ORIGIN      
        1 mnkedhnarr qaranalhsh yaskkhdfyv dtagpyhtrg ewnrvhtrrv pccrrdgcrl
       61 kvalsnfcrg lsehaaasga nrvvaalpal esagfagkvq gphqpedvaq ssdegpfgdd
      121 rrfqlvalvs almgrtdlas haesfkgsal fmdpwpaaya nmnstewkrl avllecshra
      181 lrelpapava cgrvvrsiaw dynllakrai lqlefqivhq rrsrsletqa lptlesagfa
      241 gkgqgphqpe dveqssdeet fgddcrfllv alvsahmart dlashaesik gralfmdhws
      301 aayanmnste wkrlavlfvg dgscshrtlr elpapayscg rilpcttwdy nllpkqailq
      361 gsalvmdhwp aayanmnaae wkrpvqqlvg dcsdipclpl kptipavpyv ravtstdpdh
      421 npavrgssav sewslvcdrh
//
LOCUS       XP_077506354             237 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115732 [Amblyomma americanum].
ACCESSION   XP_077506354
VERSION     XP_077506354.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650228.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..237
                     /product="uncharacterized protein LOC144115732"
                     /calculated_mol_wt=25679
     CDS             1..237
                     /gene="LOC144115732"
                     /coded_by="XM_077650228.1:1..714"
                     /db_xref="GeneID:144115732"
ORIGIN      
        1 mafgtspsgl raasrvaraa daraederhs lfkrktslkl wavlssfevr gvlapattat
       61 qparrlpapt asakgpsdsc rvvgetlrap dagardmaaa vltsaqqpqg attpeasrrv
      121 qrallklelp dwfrqhyrpp rrdagsrlgd dgaprwpkwr appakdalrp aqtatttlpa
      181 drrnlvipkr vtfrekgssr spvrnwayrs lrtpylgwrs aelgaaaptp hqaaavr
//
LOCUS       XP_077506362             443 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome P450 4V2-like [Amblyomma americanum].
ACCESSION   XP_077506362
VERSION     XP_077506362.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650236.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="cytochrome P450 4V2-like"
                     /calculated_mol_wt=50667
     Region          102..423
                     /region_name="cytochrome_P450"
                     /note="cytochrome P450 (CYP) superfamily; cl41757"
                     /db_xref="CDD:477761"
     Site            order(152,156,163,208,237,240..241,244,297,306,308,331,
                     365..367,371..375,378..379)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410651"
     CDS             1..443
                     /gene="LOC144115742"
                     /coded_by="XM_077650236.1:275..1606"
                     /db_xref="GeneID:144115742"
ORIGIN      
        1 msahekiaas asgyspvaal vllalatavl akvvmalarw irmywclrnv phpkqrwpfs
       61 laidmwqsla kmdpnleita rifnyfeemf ktihdqevtv ayygpqpfli aatpkalepv
      121 lnnsqnlnka flykmmkpwi gngiliidks lwrsrrklit pafhfrvmdd yapimnrrar
      181 emlckldavg teffdvlpim rlaafgilfg lrpkkgeral dafvpcahqs ekqayhgfet
      241 tasaiaytlf llgnhpevqa kvleeidsif gddqerdvti edmkqlkyme cvfkesmrly
      301 ppvpliarnv dedmkvgeyt vpkgavavaa iyfiqrhpky fekpdcfipe rfldtkeknp
      361 fqyipfsggl rncigqrfan leekilltqi mrrytvtskl rmdqlqlsie vvlkatqgme
      421 ikikprekvk kqaesvtgna hsq
//
LOCUS       XP_077506383            1141 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC144115757
            [Amblyomma americanum].
ACCESSION   XP_077506383
VERSION     XP_077506383.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650257.1
KEYWORDS    RefSeq; corrected model; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio   :: 5% of CDS bases
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1141
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC144115757"
                     /calculated_mol_wt=130481
     Region          104..533
                     /region_name="cytochrome_P450"
                     /note="cytochrome P450 (CYP) superfamily; cl41757"
                     /db_xref="CDD:477761"
     Site            order(155,159,166,213,342..343,346..347,350..351,354,407,
                     416,418,441,477..479,483..487,490..491)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410651"
     Site            order(239..240,342,345..346,350,414..417)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410651"
     Region          703..1136
                     /region_name="cytochrome_P450"
                     /note="cytochrome P450 (CYP) superfamily; cl41757"
                     /db_xref="CDD:477761"
     Site            order(759,763,770,817,945..946,949..950,953..954,957,1010,
                     1019,1021,1044,1080..1082,1086..1090,1093..1094)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410651"
     Site            order(843..844,945,948..949,953,1017..1020)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410651"
     CDS             1..1141
                     /gene="LOC144115757"
                     /coded_by="XM_077650257.1:313..3738"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 1 base in 1 codon"
                     /db_xref="GeneID:144115757"
ORIGIN      
        1 msiflassgl aeltsvcikg aaltlvtill ltgvfvwlar wiqwktfrhl khlphrneyl
       61 pfqglwimfk acsqpdspvr fstlvfsaic glharfqryg rhvvyvgftp lltlykaefi
      121 eeilssqkil skgvxygllh cwlgtgllts tgekwrarrr lftpafhfri ledfsptina
      181 qsiilankle rlsqyggkfd tvplvtlctl diicetimgt svsaqsnens pyvaavnria
      241 elfvertmsp flqrdfiykr tasgkefykc lnilhnftrq viserkkele aeiskgtikl
      301 dgssecetgi knrrpfldll isehlkneks itkedireev dtfmfeghdt tamgiswalf
      361 iighspeeqr kiheeldsif gddkeryvts edlrkmkyle caikeaqriy psvplisrtc
      421 eepfeidgat lpagtvvqia ayflhrnpev fpkpeefhpe rffpensngr hpfaylpfsa
      481 gprncigqrf alaeekiiia nilrrfslks ldqrdqvelv selvlrprsg lriqytrrhf
      541 agterattar taelekiema alclrrthke svttlvlsar yffpniyqig kkneclamfs
      601 ysfhdvsgll tlsgatkias fvglllalal lttlslaily lsavkfiqvk klrhlknvph
      661 rweplpymnl walyksteqa gpeflssslf savvgfhriy kkhgmhmffi gttpsvslqk
      721 aefveevlss nsllakgpey gllhnwlgtg lltstgnkwr srrrlftpaf hfriledfls
      781 tiniqstila dklgtmcrpg nhtdivplvt lctldiicet imgtsiraqy derspyvmav
      841 nslgelfirr mlnpffssef ifkrsgigre yykclntlhs ftrkviaerk tllqkqghle
      901 iqrdsdidde eqnkqkrpfl dllitehlkn pssiteehir eevdtfmfeg hdttamgisw
      961 alflighhps eqqqihdeld aifgddkqrp vtsedlremk ylesclkeaq rlypsvsfit
     1021 raceepfqig gvtfptgtvv rlstyclhrd envfpkpeef hperffaenv kgrhpfayvp
     1081 fsagprncig qkfalseeki vianilrrfk lksldqrdqv alvselvlrp knglriqfea
     1141 r
//
LOCUS       XP_077506394            1082 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115765 [Amblyomma americanum].
ACCESSION   XP_077506394
VERSION     XP_077506394.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650268.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1082
                     /product="uncharacterized protein LOC144115765"
                     /calculated_mol_wt=124751
     Region          103..531
                     /region_name="cytochrome_P450"
                     /note="cytochrome P450 (CYP) superfamily; cl41757"
                     /db_xref="CDD:477761"
     Site            order(158,162,169,215,340..341,344..345,348..349,352,405,
                     414,416,439,475..477,481..485,488..489)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410651"
     Site            order(241..242,340,343..344,348,412..415)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410651"
     Region          641..1072
                     /region_name="CYP4"
                     /note="cytochrome P450 family 4; cd20628"
                     /db_xref="CDD:410721"
     Site            order(672,689..690,697,701,708,882,885..886,889,951,955,
                     1016..1018,1022..1026,1029..1030,1034)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410721"
     Site            order(690,884..885,889,954,956)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410721"
     CDS             1..1082
                     /gene="LOC144115765"
                     /coded_by="XM_077650268.1:426..3674"
                     /db_xref="GeneID:144115765"
ORIGIN      
        1 msaggeatlf pmtltgflpf sshrvlwslt lllscivpva vhiarrrria eliskipgpt
       61 aahpilgnld vlyelkkyrh llaphilllq tmcglaqihd kdrifrfwlg frpvvsffka
      121 etvevilssn tvldksfdyt llhpwlgtgl ltssgnkwrr rrkmltpafh friledfipv
      181 fneqavifvk nlkeqqnkky idivplvtlc tldiicetam gvkvdaqlns nshyvrslye
      241 vgetfmarvm rpwlwpnyvf ymssfgrkfk dnlaqlhnft rkvirerkae lleqkvidgl
      301 tigeqvigqk rrqafldlll shhiqdsslt eedireevdt fmfeghdtta mgiswamyli
      361 glytdvqqki heeldgifge dreraitpdd lkemkyleca lkesqrlfps vpfigrelme
      421 dvvvngytvp rgttcflftf mlhrdkeifp npevfdpdrf rpencvgrhp fayvpfsagp
      481 rncigqkfal meekvvlcsv lrnfciqsvd frdkihlvae lvtrskhglk irlrphhitd
      541 psrsntectt mfalltravv wlvlssslal aclavavlga vhaarratwk wlrcfpgpne
      601 diplrwvvqq hrhassvknk tpynvsmlev riayyhmfrk hglfrfyigp hptvavfrad
      661 yleivlnsks tmeksvdyev lhswlgtgll tssgekwktr rrlltpafhf rildsfvqpm
      721 nararglvqk ilqrstdpwi divplvaect lsilletimg vfpeeeeehc rsyvravhyl
      781 ssqissrvqm pwllldaiyf rtaggkeykk sadivhnfta kvikkrreel kstdqgnlve
      841 ekeesvynrk rlctfldtll rysiendtsf tdddireevd tfmfeghdtt svaiswslym
      901 iglhqdhqrk iqeeldlile dcsgnditse nlkelkyldc vikecqrlfp svpitgresv
      961 edfklgdyti pkgstidvfi yalhrdpdvy pnperfdpsr flpencisrh pyafipfsag
     1021 srncigqrya amelkiiiat vlrnfnvial dhrdkvrlss dlvlraadgi rlsfsprfhr
     1081 st
//
LOCUS       XP_077506408             375 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC144115782
            [Amblyomma americanum].
ACCESSION   XP_077506408
VERSION     XP_077506408.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650282.1
KEYWORDS    RefSeq; corrected model; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio            :: 27% of CDS bases
            internal stop codons :: corrected 2 genomic stop codon
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..375
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC144115782"
                     /calculated_mol_wt=41057
     CDS             1..375
                     /gene="LOC144115782"
                     /coded_by="XM_077650282.1:1..1128"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: substituted 2 bases at 2
                     genomic stop codons"
                     /db_xref="GeneID:144115782"
ORIGIN      
        1 mllaensasa vgrglptsev atdsykvvlp rlptgnvvln tvfphddlqd rayrdpdfrd
       61 alaqivdlre milcngqykm shvwmvtres ssskqklidk aelhvkglkc ivfdpgtksv
      121 klkllwlpcy mehrriveal mpfgtvltie rekwrcpgme hmktgnrgis ltlkygvsan
      181 tiphtlnvvg vqalvvipgr pplclrcnrv ghvrrqcrtp rctqcrrfgh rvdncvmsya
      241 yrfrxgnxah edevvsehim dvsdvveasg elshevrted gpkasapnng kappestdkq
      301 espspdiack ygqtgiavsr pkatgpgsal vyylessacc leptchstft raerpccrvs
      361 ggasasecat cllcr
//
LOCUS       XP_077506434             180 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115810, partial [Amblyomma
            americanum].
ACCESSION   XP_077506434
VERSION     XP_077506434.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650308.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on the carboxy end.
FEATURES             Location/Qualifiers
     source          1..180
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..>180
                     /product="uncharacterized protein LOC144115810"
     Region          96..170
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..180
                     /gene="LOC144115810"
                     /coded_by="XM_077650308.1:156..>695"
                     /db_xref="GeneID:144115810"
ORIGIN      
        1 magvfrsslq ttveiavgdi tktvtavrss dgscipdgng ylyegadger yvlvfedeht
       61 pasqprasde sgigasasae avapipasae adlelwsgak trflidkyvq fkdlvgqkgg
      121 frtkkmfwtr ltnllnhefg gtlsavqven kwkslersyk rartknntsg hhrvpceyee
//
LOCUS       XP_077506445             868 aa            linear   INV 25-NOV-2025
DEFINITION  lysosomal alpha-mannosidase-like [Amblyomma americanum].
ACCESSION   XP_077506445
VERSION     XP_077506445.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650319.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..868
                     /product="lysosomal alpha-mannosidase-like"
                     /calculated_mol_wt=98220
     Region          54..763
                     /region_name="PLN02701"
                     /note="alpha-mannosidase"
                     /db_xref="CDD:178304"
     Region          54..>237
                     /region_name="GH38-57_N_LamB_YdjC_SF"
                     /note="Catalytic domain of glycoside hydrolase (GH)
                     families 38 and 57, lactam utilization protein LamB/YcsF
                     family proteins, YdjC-family proteins, and similar
                     proteins; cl49606"
                     /db_xref="CDD:483946"
     CDS             1..868
                     /gene="LOC144115817"
                     /coded_by="XM_077650319.1:223..2829"
                     /db_xref="GeneID:144115817"
ORIGIN      
        1 mpqklvlcsr lhlsaislvl fsfgnisvte kvaeddmass ctyescfvgr pdmlnvhvvc
       61 hshndvgwtq tpdviyseyv qdiyssvtda lhksrdrkyv saetafferw wqeqlpprrt
      121 svrkliqqgq lefvgggwvq sdeatthysa ivdqmtlglr wlndtfgecg rprvawqidp
      181 yghslvenaa llrpartvha faqmgfdavf lgrvhvadke yrrkqrslef lwnadrnivr
      241 pfvqflnglg rlsspvdhfv mackqiavta glrasihkfk ravatlqhhd aitgtcseka
      301 argysrligd sifqcekvis dgivsllwrn pdsyeeplqf chrmnetvcy vtenesqflv
      361 vaynplgqrv khyvrlpvss kegykvetrq hetletqvvp mantamdlpl kksiatnelv
      421 fqvdmpplga vayhvsktav rkaasenydi fkidleeasi eseryrlvvd vttgllkeii
      481 ilergetlql rqsfycyesq rsggkaglgg ptsgayifsp addepydlgm hatyriikgr
      541 vvqeiqqvfd swltqvirly rgldiiefew mlgpipiesr rskfvgreiv trfttnllnd
      601 gifytdsngr stierrrgpt arkgndnwst tasqyypvvs wvyikdvirn iqmtilpdsa
      661 qgtaslhdge velmvhrrlv eddgcglgea lndtsvvrgr hwvhfgnsew psmrqralql
      721 vyapvlayak mssesvdgal akgnfsglre elpesvhilt lealsssrll lrlehlllek
      781 edkeandkpi pitrllkgnh ledvreatlt ghmlldemrr lrwrqtqtsa antvlggsgl
      841 vrdqegykvr ladgrirtyy atlvpkar
//
LOCUS       XP_077506452             341 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115823 [Amblyomma americanum].
ACCESSION   XP_077506452
VERSION     XP_077506452.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650326.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..341
                     /product="uncharacterized protein LOC144115823"
                     /calculated_mol_wt=36913
     Region          <198..>238
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     CDS             1..341
                     /gene="LOC144115823"
                     /coded_by="XM_077650326.1:1..1026"
                     /db_xref="GeneID:144115823"
ORIGIN      
        1 mssvgavsaa qsgrgnrffa ataedyqvil pnlpsgriva ntvfmhgdpr arpyrvedyr
       61 dtlanlgalp dvvalgayqm nhvwavtmtt adaaqkllla vdvkvkdrpc llidpnnrev
      121 rvklhwllhg vtdedvrval spygkvlevt rekwralgvt ekgsttrtvg lklhadvkid
      181 diphqlkiag eltlvvvpgr aplclrckst ghirrdckvp rcsrcrrfgh vdadcaktya
      241 svagpvqand isdhlmdeed seetartsrn lvtqgassnf qgeqlidggd kpadkrrhdd
      301 aeggnekdea sttghpspgg sekpptepap vtvmdsdsge r
//
LOCUS       XP_077506463             336 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115831 [Amblyomma americanum].
ACCESSION   XP_077506463
VERSION     XP_077506463.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650337.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..336
                     /product="uncharacterized protein LOC144115831"
                     /calculated_mol_wt=37432
     Region          35..109
                     /region_name="GT1"
                     /note="myb-like, SANT family; cl23759"
                     /db_xref="CDD:474047"
     CDS             1..336
                     /gene="LOC144115831"
                     /coded_by="XM_077650337.1:576..1586"
                     /db_xref="GeneID:144115831"
ORIGIN      
        1 msdldisdfs ttpsasasda tqrakkrpac prfiftvder hmfrnimaky rdvienkrtd
       61 nttkkakdea wsqlcddyns lagtrtvcva qlrklwdnmk srwkkkksee treifrtggg
      121 tlecrpmspa telvgavadh matrlpnpfd sdgahvseav lslppvalle amandnepsq
      181 ddfsskycmc vhvfagevpa ptsmeaapsp wlnsrpltde alpvqeecls agrptvlvpv
      241 vpscqsvsed itpaqiartr aggprvaave rtlapevaar ikaieaedrr keelhqldlq
      301 lrrsqlaeqr lknkmqrkll sldfeikkrq lealkr
//
LOCUS       XP_077506470             213 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115840 [Amblyomma americanum].
ACCESSION   XP_077506470
VERSION     XP_077506470.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650344.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..213
                     /product="uncharacterized protein LOC144115840"
                     /calculated_mol_wt=23722
     CDS             1..213
                     /gene="LOC144115840"
                     /coded_by="XM_077650344.1:1..642"
                     /db_xref="GeneID:144115840"
ORIGIN      
        1 mnhvwavtlk deerekeilq geafdvkehr cvvidpsnqg vrlklywllh gmrdndvrsa
       61 lapfgkvtdi aknkwkvsgc vdkastthsv tlklnaglti edlppqlrva evvalvyvhg
      121 raplclrwrg tghirrecrv srcgicrrfd hdetqcvrty gsvagparkd emaehlmdev
      181 daaeatregs iegtskdtpp elpgpdgmrt vaw
//
LOCUS       XP_077506481             208 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115846 [Amblyomma americanum].
ACCESSION   XP_077506481
VERSION     XP_077506481.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650355.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 38% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..208
                     /product="uncharacterized protein LOC144115846"
                     /calculated_mol_wt=21977
     Region          121..175
                     /region_name="bHLH_SOHLH_like"
                     /note="basic helix-loop-helix (bHLH) domain found in the
                     spermatogenesis- and oogenesis-specific basic
                     helix-loop-helix-containing protein (SOHLH) family and
                     similar proteins; cd19683"
                     /db_xref="CDD:381526"
     Site            order(121..124,127..128,130..131,153..154)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381526"
     Site            order(131,133..134,137..138,140..141,144,154..156,
                     158..159,161..162,164..166,168..169)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:381526"
     CDS             1..208
                     /gene="LOC144115846"
                     /coded_by="XM_077650355.1:1..627"
                     /db_xref="GeneID:144115846"
ORIGIN      
        1 meyiilevfp gvtkhhfavv dtleaavraa ylplpassha adstaanlga gprtsvaaaa
       61 agtdgtlhaa lggddvtaaa agadganndi mgvtrvanmg qmqhmvgpld nmpyympfng
      121 renheqerkr rtriknacqt lrslvpalse qtdkatvfef tvqyllhlrg hlgskhdksi
      181 sqpalfgfwk aalssnesgt gsicqtdd
//
LOCUS       XP_077506512             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115849 [Amblyomma americanum].
ACCESSION   XP_077506512
VERSION     XP_077506512.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650386.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144115849"
                     /calculated_mol_wt=24225
     Region          10..104
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     CDS             1..217
                     /gene="LOC144115849"
                     /coded_by="XM_077650386.1:1..654"
                     /db_xref="GeneID:144115849"
ORIGIN      
        1 msrglfnevl iievskrpll wdvkdnfrnk stkeslweev rdairaiddt vtveeiiarw
       61 knlkdtyrrk tkdekdgkks gsgataktaw phlkqmeflr dsmetrrsfc nldavshvqv
      121 asaaaetsha scspaspgsk eveaspnefe cmysvpglpd epgpscmaat spevlphrgn
      181 krqkkrrqev eaadrdiqav rsaiaahrpm vtphvrt
//
LOCUS       XP_077506527             334 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115850 [Amblyomma americanum].
ACCESSION   XP_077506527
VERSION     XP_077506527.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650401.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 32% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..334
                     /product="uncharacterized protein LOC144115850"
                     /calculated_mol_wt=35721
     Region          30..113
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          <111..223
                     /region_name="PLN02217"
                     /note="probable pectinesterase/pectinesterase inhibitor"
                     /db_xref="CDD:215130"
     CDS             1..334
                     /gene="LOC144115850"
                     /coded_by="XM_077650401.1:1..1005"
                     /db_xref="GeneID:144115850"
ORIGIN      
        1 mgsqqfclkw nnhqsnmlav fyqmlsneal vdvtlacegl slkahkmvls acspffqalf
       61 aenpckhpiv ilkdvryvdl raivdfmyrg evvvpqedlt allktaetlk vkglvtsvdg
      121 aadsgpvgsp raespplskr krsrrprrcs psdsnksdse dappatrikv pdspeiiedg
      181 smssdhmtdp htaspasras nvasstavps galhgssvqa sisqptqdsi gddvgegdda
      241 dfdvepldvm eesattesvp vfpdvasssq algdesqqsh htgstsgnsa lvpaqpslps
      301 dipitsqedi epsptsltpy ddwfkhwcds fkhq
//
LOCUS       XP_077506542             288 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115862 [Amblyomma americanum].
ACCESSION   XP_077506542
VERSION     XP_077506542.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650416.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 11% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..288
                     /product="uncharacterized protein LOC144115862"
                     /calculated_mol_wt=32673
     Region          71..275
                     /region_name="Cut8"
                     /note="nuclear proteasome tether protein; pfam08559"
                     /db_xref="CDD:462518"
     CDS             1..288
                     /gene="LOC144115862"
                     /coded_by="XM_077650416.1:78..944"
                     /db_xref="GeneID:144115862"
ORIGIN      
        1 mpyfgspsps tppdeevvrr rgrriispgn qfaltppqtp tkrqlppkss sstehpqtps
       61 kaqlslspvr ssprkrlllp aeisdaigas pskklvspvk rlrlqsrdae aetevaltvl
      121 tkaqlvtllk dtmgrspdak riihellpkp dlahsmqkle kllhqiyraf pqkhwgdsrd
      181 afcfrrvqip ietfkrkcle raqqlcqaeq wcsllqyaqq awnfvrkmpd wedashnkak
      241 qlcfrslath sttalkkasq rntkwrpqmr qsgwltsalr pgslrppr
//
LOCUS       XP_077506555             191 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115867 [Amblyomma americanum].
ACCESSION   XP_077506555
VERSION     XP_077506555.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650429.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..191
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..191
                     /product="uncharacterized protein LOC144115867"
                     /calculated_mol_wt=21237
     CDS             1..191
                     /gene="LOC144115867"
                     /coded_by="XM_077650429.1:1..576"
                     /db_xref="GeneID:144115867"
ORIGIN      
        1 mgdptasscf frrrlksrfy lvvqseriti ieaseghgpp ernlhetsgp kidednlsrg
       61 dergasascr qdtrsptdpt pvpspqpsqq htpepplqps tratpsllgk rrrddgankv
      121 lrqmlyeshr ldsltdarnr qdaafqqsml eaiwevtdav qasngqqell iqnvnlltli
      181 lqkqleppap h
//
LOCUS       XP_077506585             423 aa            linear   INV 25-NOV-2025
DEFINITION  chromosome-associated kinesin KIF4A-like [Amblyomma americanum].
ACCESSION   XP_077506585
VERSION     XP_077506585.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650459.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..423
                     /product="chromosome-associated kinesin KIF4A-like"
                     /calculated_mol_wt=46594
     Region          <10..240
                     /region_name="KISc"
                     /note="Kinesin motor, catalytic domain. ATPase;
                     smart00129"
                     /db_xref="CDD:214526"
     Region          <245..>417
                     /region_name="GumC"
                     /note="Exopolysaccharide export protein/domain GumC/Wzc1
                     [Cell wall/membrane/envelope biogenesis]; COG3206"
                     /db_xref="CDD:442439"
     CDS             1..423
                     /gene="LOC144115868"
                     /coded_by="XM_077650459.1:44..1315"
                     /db_xref="GeneID:144115868"
ORIGIN      
        1 mgircsknad degiipraig difknmtttt gksflvrvsf leiyqeqifd llsnsrgrvp
       61 lairedktgt vkvpnltqhk vatadetlrl vgigsasrit aatnmnicss rshailtltv
      121 eqqnesggat tvakmnfvdl agseraektk aigerfkegm kinhgllslg nvvsslserk
      181 fhvpyrnskl trllqdslgg sshtvmiaci spasgnredt ldtlryadra rkiknkptan
      241 idptqaeian lrqklqeaea almrctthev qnpiasggae daellkdklk lalahnaqle
      301 tenrrlaakl qqaqdqarkq seqvispmek tvsklgeeha paecmsrike leakvqrler
      361 gferkilevk kelqelslfk hsansttesh qdicaslveg legqaqeiyk lqnaqdepkk
      421 kte
//
LOCUS       XP_077506595             319 aa            linear   INV 25-NOV-2025
DEFINITION  chromosome-associated kinesin KIF4A-like [Amblyomma americanum].
ACCESSION   XP_077506595
VERSION     XP_077506595.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650469.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..319
                     /product="chromosome-associated kinesin KIF4A-like"
                     /calculated_mol_wt=35262
     Region          <1..130
                     /region_name="Motor_domain"
                     /note="Myosin and Kinesin motor domain; cl22853"
                     /db_xref="CDD:473979"
     Region          <131..>308
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..319
                     /gene="LOC144115875"
                     /coded_by="XM_077650469.1:1..960"
                     /db_xref="GeneID:144115875"
ORIGIN      
        1 mnicssrsha iltltveqqn esggattvak mnfvdlagse raektkaige rfkegmkinh
       61 gllslgnvvs slserkfhvp yrnskltrll qdslggssht vmiacispas gnredtldtl
      121 ryadrarkik nkptanidpt qaeianlrqk lqeaeaalmr ctthevqnpi asggaedael
      181 lkdklelala hnaqletenr rlaaklqqaq dqarkqseqv ispmektvsk lgeehapaec
      241 msrikeleak vqrlergfer kilevkkelq elslfkhsan stteshqdic aslveglegq
      301 aqeiyklqna qdepkkkte
//
LOCUS       XP_077506610             423 aa            linear   INV 25-NOV-2025
DEFINITION  chromosome-associated kinesin KIF4A-like [Amblyomma americanum].
ACCESSION   XP_077506610
VERSION     XP_077506610.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650484.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 12% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..423
                     /product="chromosome-associated kinesin KIF4A-like"
                     /calculated_mol_wt=46595
     Region          <10..240
                     /region_name="KISc"
                     /note="Kinesin motor, catalytic domain. ATPase;
                     smart00129"
                     /db_xref="CDD:214526"
     Region          <235..>412
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..423
                     /gene="LOC144115880"
                     /coded_by="XM_077650484.1:1..1272"
                     /db_xref="GeneID:144115880"
ORIGIN      
        1 mgircsknad degiipraig difknmtttt gksflvrvsf leiyqekifd llsnsrgrvp
       61 lairedktgt vkvpnltqhk vatadetlrl vgigsasrit aatnmnicss rshailtltv
      121 eqqnesggat tvakmnfvdl agseraektk aigerfkegm kinhgllslg nvvsslserk
      181 fhvpyrnskl trllqdslgg sshtvmiaci spasgnredt ldtlryadra rkiknkptan
      241 idptqaeian lrqklqeaea almrctthev qnpiasggae daellkdkle lalahnaqle
      301 tenrrlaakl qqaqdqarkq seqvispmek tvsklgeeha paecmsrike leakvqrler
      361 gferkilevk kelqelslfk hsansttesh qdicaslveg legqaqeiyk lqnaqdepkk
      421 kte
//
LOCUS       XP_077506622             393 aa            linear   INV 25-NOV-2025
DEFINITION  chromosome-associated kinesin KIF4A-like [Amblyomma americanum].
ACCESSION   XP_077506622
VERSION     XP_077506622.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650496.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..393
                     /product="chromosome-associated kinesin KIF4A-like"
                     /calculated_mol_wt=43236
     Region          <10..210
                     /region_name="Motor_domain"
                     /note="Myosin and Kinesin motor domain; cl22853"
                     /db_xref="CDD:473979"
     Region          <214..>382
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..393
                     /gene="LOC144115883"
                     /coded_by="XM_077650496.1:1..1182"
                     /db_xref="GeneID:144115883"
ORIGIN      
        1 mgircsknad degiipraig difknmtttt gksflvrvsf levpnltehk vatadetvrl
       61 vvigsasrit aatnmnicss rshailtltv eqqnesggat tvakmnfvdl agseraektk
      121 aigerfkegm kinhgllslg nvvsslserk fhvpyrnskl trllqdslgg sshtvmiaci
      181 spasgnreet ldtlryadra rkiknkptvn idptqaeian lrqklqeaea almrctthev
      241 qnpiasggae daellkdkle lalahnaqle tenrrlaakl qqaqdqarkq seqvispmek
      301 tvsklgeeha paecmsrike leakvqrler gferkilevk kelqelslfk hsansttesh
      361 qdicaslveg legqaqeiyk lqnaqdepkk kte
//
LOCUS       XP_077506634             423 aa            linear   INV 25-NOV-2025
DEFINITION  chromosome-associated kinesin KIF4A-like [Amblyomma americanum].
ACCESSION   XP_077506634
VERSION     XP_077506634.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650508.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 11% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..423
                     /product="chromosome-associated kinesin KIF4A-like"
                     /calculated_mol_wt=46547
     Region          <10..240
                     /region_name="KISc"
                     /note="Kinesin motor, catalytic domain. ATPase;
                     smart00129"
                     /db_xref="CDD:214526"
     Region          <235..>412
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..423
                     /gene="LOC144115887"
                     /coded_by="XM_077650508.1:1..1272"
                     /db_xref="GeneID:144115887"
ORIGIN      
        1 mgircsknad degiipraig difknmtttt gksvlvrvsf leiyqeqifd llsnsrgrvp
       61 lairedktgt vkvpnltqhk vatadetlrl vgigsasrit aatnmnicss rshailtltv
      121 eqqnesggat tvakmnfvdl agseraektk aigerfkegm kinhgllslg nvvsslserk
      181 fhvpyrnskl trllqdslgg sshtvmiaci spasgnredt ldtlryadra rkiknkptan
      241 idptqaeian lrqklqeaea almrctthev qnpiasggae daellkdkle lalahnaqle
      301 tenrrlaakl qqaqdqarkq seqvispmek tvsklgeeha paecmsrike leakvqrler
      361 gferkilevk kelqelslfk hsansttesh qdicaslveg legqaqeiyk lqnaqdepkk
      421 kte
//
LOCUS       XP_077506656             414 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115896 [Amblyomma americanum].
ACCESSION   XP_077506656
VERSION     XP_077506656.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650530.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 36% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..414
                     /product="uncharacterized protein LOC144115896"
                     /calculated_mol_wt=45723
     CDS             1..414
                     /gene="LOC144115896"
                     /coded_by="XM_077650530.1:1..1245"
                     /db_xref="GeneID:144115896"
ORIGIN      
        1 mpkdssptsa alpptvvcag alrqrdpavf sgtgdhdved wlslyervsi hnkwddarkl
       61 nnvifyltdv anlwyrnlea ditlwsafkq nfagvfglpa vrklraeqrl raraqlpgen
      121 ftsyiedvvy lcrrvdssmp erakidhilk gidddafqml laknpatvvd vlaqcqsyde
      181 lrkrhlltrs plsnsepmga ltsdcdhspl lpqikefire evarqlslvn pspdassplm
      241 pslqrvireq vaealptlpe vrsvpplsya eaaahynpst pvaapltyaa vaaqphpvaf
      301 spsppvlrpa pvppaaplhp pssphynpwc tpdnrpicfs cgfpghvarh crrrmvappa
      361 fapsaglpql lraapppeay asdqrpyrtr rspsprhrsl spirrrptpa yegn
//
LOCUS       XP_077506666             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115904 [Amblyomma americanum].
ACCESSION   XP_077506666
VERSION     XP_077506666.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650540.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 26% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144115904"
                     /calculated_mol_wt=15667
     CDS             1..133
                     /gene="LOC144115904"
                     /coded_by="XM_077650540.1:61..462"
                     /db_xref="GeneID:144115904"
ORIGIN      
        1 mrkypamffr yyrmnpqqfd garkivrvdl erhhlcrviv cseerlaial rflssgmaik
       61 qvamvfrvaq stcrlvihdt craiwkhlep lympasigdl lfcskyvryv lrekvelghv
      121 tnhlaaqprr rfr
//
LOCUS       XP_077506673             498 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115915 [Amblyomma americanum].
ACCESSION   XP_077506673
VERSION     XP_077506673.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650547.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..498
                     /product="uncharacterized protein LOC144115915"
                     /calculated_mol_wt=53833
     Region          <8..61
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Region          225..346
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     Site            order(228..231,260..262,265,289,328,346)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260008"
     CDS             1..498
                     /gene="LOC144115915"
                     /coded_by="XM_077650547.1:1..1497"
                     /db_xref="GeneID:144115915"
ORIGIN      
        1 mevfctvyad dvalwvrasr rhiasarqvl qraldevqcf rasrglvlsa tksealfvhp
       61 rgaratsrir tlrltastsp ggsktnrlrr letsrrkwir rlvgvpqssh iaatlaeagv
      121 lplnllllqr glthtdrlhh vpdcralrar lsgrpapgwa csplptwspw alppashpal
      181 pvpptrpplq vsldfggtsk rhtpaaainq taaafldeik gstllftdgs vlpatgqgaa
      241 slvapqlnat rtcrlpfpgs spaaelaglh laadlnattp pgtvvvlcds rpalqllsrp
      301 ldnvaatsll rdrlqtlega ghqislhwlp ghsgiegndl adalarsfht ngspvcfavt
      361 qhdfarllim qmvgalhpdr riaackhfrr lsdqlprrdr allqrlpigc twtpsrlyac
      421 grahspacps cedtgtlghl llacpthdtp rrrlesgyra lglpsttehe llfparsqll
      481 ahrvllsfle ktglsill
//
LOCUS       XP_077506683             414 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115924 [Amblyomma americanum].
ACCESSION   XP_077506683
VERSION     XP_077506683.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650557.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 42% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..414
                     /product="uncharacterized protein LOC144115924"
                     /calculated_mol_wt=45890
     CDS             1..414
                     /gene="LOC144115924"
                     /coded_by="XM_077650557.1:1..1245"
                     /db_xref="GeneID:144115924"
ORIGIN      
        1 mpkdssptsa alpptfvcag alrqrdpavf sgtgdhdved wlssyervsi hykcddarkl
       61 nnvifyltdf anlwyrnlea ditlwsafkq nfagvfglpa vrklraeqrl raraqlpgen
      121 ftsyiedvvy lcrrvdssmp erakidhilk giddnafqml laknpatvvd vlaqcqsyde
      181 lrkrhlltrs plsksepmga ltsdcdhspl lpqikefire evarqlslvn pspdassllm
      241 pslqrvireq vaealptlpe vrsvpplsya eaaahynpst pvaapltyaa vaaqphpvaf
      301 spsppflrpa pvppaaplhp pssphynpwr tpdnrpicfs cgfpghvarh crrrmvappa
      361 fapsaglpql lraapppeay asdqrpyrtr rspsprhrsl spirrrptpa yegn
//
LOCUS       XP_077506709             367 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115946 [Amblyomma americanum].
ACCESSION   XP_077506709
VERSION     XP_077506709.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650583.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..367
                     /product="uncharacterized protein LOC144115946"
                     /calculated_mol_wt=40938
     Region          4..79
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          <187..>324
                     /region_name="DUF3584"
                     /note="Protein of unknown function (DUF3584); pfam12128"
                     /db_xref="CDD:432349"
     Region          <232..>297
                     /region_name="PRK13922"
                     /note="rod shape-determining protein MreC; Provisional;
                     cl19252"
                     /db_xref="CDD:473155"
     CDS             1..367
                     /gene="LOC144115946"
                     /coded_by="XM_077650583.1:220..1323"
                     /db_xref="GeneID:144115946"
ORIGIN      
        1 mpgccvpqcs nhcrkgtrmf rfpadsnrrk rwlaqvkrdc weptaasric sahfeetsfe
       61 qnrqdglkkl rpdavptlft yraipkhrkp pkkrscfrpa tptqspvldp tgnepcgsaa
      121 lepvfastae qtqdsavsds mssqdtvspl qcaqvpgydl pltttllppk avdtviyaes
      181 vahsflrtdk asgedstdas rlplpaaspm eaeslsaers qhsnersske ipqaaftlgs
      241 stastdlslq rqnaelrkrn kdlaqkyrnl qrlheqtkrk lrslvkksqs pqqkmitleq
      301 lpflkhdqrr altvkttkgl kwliehyvet tcvgarhhar svvprarpfv lsarlngntk
      361 ykghiae
//
LOCUS       XP_077506727             752 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115959 [Amblyomma americanum].
ACCESSION   XP_077506727
VERSION     XP_077506727.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650601.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..752
                     /product="uncharacterized protein LOC144115959"
                     /calculated_mol_wt=82045
     Region          32..244
                     /region_name="RT_nLTR_like"
                     /note="RT_nLTR: Non-LTR (long terminal repeat)
                     retrotransposon and non-LTR retrovirus reverse
                     transcriptase (RT). This subfamily contains both non-LTR
                     retrotransposons and non-LTR retrovirus RTs. RTs catalyze
                     the conversion of single-stranded RNA into...; cd01650"
                     /db_xref="CDD:238827"
     Site            order(122..127,150,159,191,193..194,240..241)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238827"
     Site            order(122..127,150,193)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238827"
     Site            159
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238827"
     Region          448..575
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     Site            order(451..454,484..486,489,514,553,571)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260008"
     Region          <640..669
                     /region_name="zf-RVT"
                     /note="zinc-binding in reverse transcriptase; pfam13966"
                     /db_xref="CDD:433612"
     Region          <707..735
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..752
                     /gene="LOC144115959"
                     /coded_by="XM_077650601.1:1..2259"
                     /db_xref="GeneID:144115959"
ORIGIN      
        1 mlrnlgedhw asllssfnev wrtgilpdaw rtalvvpvlk mgkppsaiss yrpvsltsvp
       61 gkvmeamale rlswvgivtg hfpeqqsgfr hhrctadsia dlvstledak lnrhvvclal
      121 ldiqgafdni rheailealn imsisnnllq yvrgvsvwgs vlspllfnav lallpnflpk
      181 dlrlpihvai yaddiaiwpq hlpfktqaml lntrvgarpg rtflhvggrd lawsrllkyl
      241 gllidvrltw npavrhflaq ssriqgairs llakgngitp rlglqlyqgm avpqstyalp
      301 lvrlsvaqhl aierklraai rlclglprss pvaatlaeag swplellsrr tslrhierlh
      361 kapdgrplld rlltrpnskm gqaaqyfedl vggqpesfpl pprpdlghly evhpslpgsr
      421 tkrntaavgl rqevaavlqa eppgtvpift dgsvlpepcp svaaactass lhssrqsrll
      481 fvassttael aaihlaadil nehpgikeaa iytdsraalq lllrpfkgpp iarrlawrld
      541 elctrgtrir ltwapahvgv lgneeadsla kaerspgtpl sknlcsfdlv rsivdrllll
      601 dhpdprvang hpphllpnlg lhckdrslll rlrvgcanig srlfriglsd spgcslcnyp
      661 eettqhllvi pksdrvvvvv lfvcgvgiag aassygvvgg gsssfvwslr lqefitvvyk
      721 sgrkhtdadc lsrapvdkpr eeddedaffg aq
//
LOCUS       XP_077506738             586 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115966 [Amblyomma americanum].
ACCESSION   XP_077506738
VERSION     XP_077506738.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650612.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 50% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..586
                     /product="uncharacterized protein LOC144115966"
                     /calculated_mol_wt=63540
     Region          <198..>238
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     Region          <392..>425
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     CDS             1..586
                     /gene="LOC144115966"
                     /coded_by="XM_077650612.1:1..1761"
                     /db_xref="GeneID:144115966"
ORIGIN      
        1 mssvgavsaa qsgrgnrffa ataddyqvil pnlpsgriva ntvfmhgdpr arpyrvedyr
       61 dtlgnlgalp dvvalgayqm nhvwavtmtt adaaqkllla vdvkvkdrpc llidpnnrev
      121 rvklhwlphg vtdedvrval spygkvlevt rekwralgvt ekgsttrtvg lklhadvkid
      181 diphqlkiag eltlvvvpgr aplclrckst ghirrdckvp rcsrcrrfgh vdadcaktya
      241 svagpvqand isdhlmdeed seetartsrn lvtqgassnf qgeqlidggd kpadkrrhnd
      301 aeggnekded vrlklhwlly nvpdedvrva iapygnvsev arerwrvhgv lekgsitrhi
      361 tlklkagvkl ddlshelnvg vklalvvpgk pplclrcrgt ghirkecpvr rcgacrrfgh
      421 kdahcartya siagpwnsed ssellmdead aeeaaratgq ilaqkapslt pllkksattt
      481 savkpsedag lepqqesddk aenatkaave slesveanht damdvsqeaa glsagkrshe
      541 esagekpqpc vggdepppkt agsrrstlkh rpnfsaesrq agkpps
//
LOCUS       XP_077506743             312 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115974 [Amblyomma americanum].
ACCESSION   XP_077506743
VERSION     XP_077506743.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650617.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..312
                     /product="uncharacterized protein LOC144115974"
                     /calculated_mol_wt=35012
     Region          172..311
                     /region_name="MATH"
                     /note="MATH (meprin and TRAF-C homology) domain; an
                     independent folding unit with an eight-stranded
                     beta-sandwich structure found in meprins, TRAFs and other
                     proteins. Meprins comprise a class of extracellular
                     metalloproteases which are anchored to the membrane...;
                     cl02446"
                     /db_xref="CDD:445786"
     CDS             1..312
                     /gene="LOC144115974"
                     /coded_by="XM_077650617.1:37..975"
                     /db_xref="GeneID:144115974"
ORIGIN      
        1 mtdsvapshd evegdfnpqv saggdggqat tsdvsnpttl tptsvnpsdd allfllsgyi
       61 kpvnrflcti fkgindvyda leefepedlt lvlkdceelr asmreeclgw gairdqiqqe
      121 vakvfdairg qvhimsgedl fedpvgkkid glmnmgkhil rerirdsvpv qwhldrwsel
      181 karavatgta adyysgirtt ffgyfivpgl llektasklm lrfsfyvrkg dfddflqwpi
      241 ekeftlsilh ptdkekvrrl vvdtrmglte ryarpgnetr epvsstkslk anyidangfv
      301 egdklllqfe vk
//
LOCUS       XP_077506773             224 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115991 [Amblyomma americanum].
ACCESSION   XP_077506773
VERSION     XP_077506773.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650647.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..224
                     /product="uncharacterized protein LOC144115991"
                     /calculated_mol_wt=25103
     Region          88..224
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..224
                     /gene="LOC144115991"
                     /coded_by="XM_077650647.1:1..675"
                     /db_xref="GeneID:144115991"
ORIGIN      
        1 mnmrlaisve krvavamykp cssaenrfva nhfglgrstv nticwefcda vvcllddkwi
       61 hmpssdamgd hirefsavcd ypqaigaldg chlpvsppke sasdyynykg cimllalvdh
      121 ryrfryvnvg spgrchdvyi yqrsqlaefi egptfqapav viggtavppl ilcdqafrlt
      181 shlikpfshs ahldadmkkl kyhtlkarrv vgntfgrlka rfrf
//
LOCUS       XP_077506782             495 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115995 [Amblyomma americanum].
ACCESSION   XP_077506782
VERSION     XP_077506782.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650656.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..495
                     /product="uncharacterized protein LOC144115995"
                     /calculated_mol_wt=55084
     Region          127..259
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     Region          <356..>431
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     CDS             1..495
                     /gene="LOC144115995"
                     /coded_by="XM_077650656.1:1..1488"
                     /db_xref="GeneID:144115995"
ORIGIN      
        1 meklkakrrs rrgqntiiin eatavlptad vhqltallhr lqvsndelrk lnddmedhlp
       61 eeafetefte aieyddramn tigllkaria asqpttpavh saesmqaslp tprhsgiklp
      121 klhlesfngg lanwqtfwec fretvhenrs lskaekchyl kslltgpass avrglqatea
      181 cyddavqilq rrfgdkgqie qehltmlril psvsssgdvr glrrlndhvq ahicglaglg
      241 vtthaysaml cdivqtalpr dmvvefhrlt sraaktdals sagsspaspa qqsasthted
      301 rsdgrlneil efllvetecr erckqdpvtv aekkpyhidr fgkrpnqhta saavlstqse
      361 kaekcafcds skhgtefcta altnkektek lakakrcfrc tkwghrskdc rrkiscehce
      421 rrhatsmcnp nwtidktdap tevtatnvha aanrketqet alqtfrafav gdtkygyirg
      481 vmdggsqktf lredi
//
LOCUS       XP_077506793             401 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116007 [Amblyomma americanum].
ACCESSION   XP_077506793
VERSION     XP_077506793.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650667.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..401
                     /product="uncharacterized protein LOC144116007"
                     /calculated_mol_wt=42944
     Region          <10..>152
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(48..53,107..108,140,142..143)
                     /site_type="active"
                     /db_xref="CDD:238827"
     Site            order(48..53,107,142)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238827"
     Site            108
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238827"
     Region          176..299
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     Site            order(177..180,209..211,214,239,278,296)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260008"
     CDS             1..401
                     /gene="LOC144116007"
                     /coded_by="XM_077650667.1:1..1206"
                     /db_xref="GeneID:144116007"
ORIGIN      
        1 madvrgavap eqsgfrhsrc tadciaeisa yleeakykkq aaylvlldiq safdslahas
       61 ildalinkgv aenlyryvks flsgrtlrvk vgdsvstprh itkgvpqgsv lspflfnivl
      121 anipnliprs plhqvrvsiy addialycsg ltlsgrtket aallhedcsg rveintdgsv
      181 leggasaaaa cvvpslgiar qscltcsass ttaevaalhl aadliletpd lspavilsds
      241 raalttlrra hhtlpmyanl dsklrqarel grdvllqwlp shagisgneq adqlakqahv
      301 dgtpectams pfdvarhnik arivaqhpda rvaagkalkp issrdfdlid rttllrmrig
      361 casvdttdeg agtsfegqeh invpeirlly vvpkahseec r
//
LOCUS       XP_077506801             371 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116014 [Amblyomma americanum].
ACCESSION   XP_077506801
VERSION     XP_077506801.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650675.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 32% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..371
                     /product="uncharacterized protein LOC144116014"
                     /calculated_mol_wt=41547
     CDS             1..371
                     /gene="LOC144116014"
                     /coded_by="XM_077650675.1:1..1116"
                     /db_xref="GeneID:144116014"
ORIGIN      
        1 mrdlrrfate wkeknmarsm felfdmisaf fpleilrenh lvriegargl apaesptpta
       61 vvaeprdpgt fcvtddvdve nwlamfervs nhnrwdetli lanvivylag tarvwfethe
      121 edltswgtck qklrdlfgkp igrkfgakkd latraqsste syisyiqyvl alcrkvdsam
      181 seaekvghil kgiaddafnl llckdcatvd svlqvyrqfe qakqrriahr fdrlpntaat
      241 sscedlptvq qpsnpanitr ivrreleamt pvafapqppp atialiqavv hqefanvgln
      301 sglpyalpct ptiaplvaaa sptpatppry hyrnpadwrt sddrpicftc spvdhisrhc
      361 hsrtqalfkp p
//
LOCUS       XP_077506809             511 aa            linear   INV 25-NOV-2025
DEFINITION  nuclear hormone receptor FTZ-F1 beta-like [Amblyomma americanum].
ACCESSION   XP_077506809
VERSION     XP_077506809.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650683.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..511
                     /product="nuclear hormone receptor FTZ-F1 beta-like"
                     /calculated_mol_wt=55089
     Region          100..192
                     /region_name="NR_DBD_like"
                     /note="DNA-binding domain of nuclear receptors is composed
                     of two C4-type zinc fingers; cl02596"
                     /db_xref="CDD:413390"
     Site            order(100,103,117,120,136,142,152,155)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143484"
     Site            order(110..112,118..119,121,123,126,129,149..150,153,156,
                     167,170)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143484"
     Region          305..462
                     /region_name="NR_LBD_F2"
                     /note="Ligand-binding domain of nuclear receptor family 2;
                     cd06930"
                     /db_xref="CDD:132728"
     Site            order(306..307,310,313..314,343..344,347..348,351,354,364,
                     387)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132728"
     Site            order(318,322,332..333,335..336,339..340)
                     /site_type="other"
                     /note="coactivator recognition site [polypeptide binding]"
                     /db_xref="CDD:132728"
     CDS             1..511
                     /gene="LOC144116020"
                     /coded_by="XM_077650683.1:1..1536"
                     /db_xref="GeneID:144116020"
ORIGIN      
        1 mrrvvqacrg pkarlrratl avalrasfrr astdaegqvk mgqpassasv assasdesna
       61 sdlssttrrp saeggggdpl galgespgdv srqqqlsaqc picgdrisgf hygalscesc
      121 kcffmrtvqi krhyvcprga tcpvvmatrk mcpacrfdkc lragmkqeav rqhgtrgrrr
      181 tyqcsyseps qllggdarla sdsgglpaep ipllqkesrs lssgsplegs pqpfvgqqqp
      241 pvvpslvqei vsvedlwhyt dcefsrlsep fghkvsatga taldaggpds pgalgadggp
      301 sllrsvcnia dlrvykivkw ckslplfrdi qeddqialls nswyelllls ccyrstgtpn
      361 girfsmgksv tlgqarhlgm gpviermlgl tdllrrlqvd qrefvclkvi illtsdasgl
      421 repekarsck kqvlealqay tisqypsqpt kfgelllcvp eleraclvsk esmapfwria
      481 ssagasslsa pmadpqtssf nlllellrgd h
//
LOCUS       XP_077506830             126 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116036 [Amblyomma americanum].
ACCESSION   XP_077506830
VERSION     XP_077506830.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650704.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 32% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..126
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..126
                     /product="uncharacterized protein LOC144116036"
                     /calculated_mol_wt=13664
     CDS             1..126
                     /gene="LOC144116036"
                     /coded_by="XM_077650704.1:36..416"
                     /db_xref="GeneID:144116036"
ORIGIN      
        1 mvdsqsagal plaqanassf ttevlaslpl vaewtgvrew ireanasrlr rrswrhyhws
       61 qsglayengy grpmpavydg gpgattpggr vdwrmrmdtg geakvnasrp ggkrpwwtar
      121 apapsl
//
LOCUS       XP_077506841             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116054 [Amblyomma americanum].
ACCESSION   XP_077506841
VERSION     XP_077506841.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650715.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 39% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144116054"
                     /calculated_mol_wt=20072
     Region          <61..110
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..181
                     /gene="LOC144116054"
                     /coded_by="XM_077650715.1:1..546"
                     /db_xref="GeneID:144116054"
ORIGIN      
        1 mihrvcgana sagnaeaerq qttanaqaip klrdrystrr tnwsqleqcd qrhvahvllr
       61 evvqhiwlel lekprenvhk tyrlcskhft rdcftsdact rltreavpsv kvqeprlral
      121 vhpdfyeegv aqaigsaass qstavpvevm lghdylplpp tvepatiaga dsdvydtasv
      181 e
//
LOCUS       XP_077506847             750 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077506847
VERSION     XP_077506847.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650721.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..750
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=83742
     Region          90..226
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          320..721
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..750
                     /gene="LOC144116060"
                     /coded_by="XM_077650721.1:1..2253"
                     /db_xref="GeneID:144116060"
ORIGIN      
        1 mkgrtaraac yvvalvvvae lwpgvagqah rrsaaipnep tttteapdne hafsadelre
       61 larsmmrdat kklfpyvqdl aydqnlshec isslfklsrg irsnklwafk fldamgrppa
      121 gllagstgal gaydeclais vpggdeptsa eafrgqyctl yvkpemtdrl mdvlqrlldq
      181 yppalrkraq nvtqlkrfyd hwlvggmrlg vclpsrcsgk dlkymvdkfa kpfrintkvs
      241 acavqkpvtf ttlqlslicm lcvlglllls atslelwltf vqsgskkdpd kplclrlllt
      301 fsvidntrtl frragretea hrlsclhgir visylwvtlc hnyaiidphv tvqgmkildi
      361 idgfaftvvv ngmlsvetff imsgflvaig ilnevkkkkk siwfllpvta vmryirltvp
      421 gvflvgiffl yplvvsgplv ddlvqylvnn vcrrnwwhvl thvsnlrdpv dvclptlwyi
      481 acdyqlflat lllvfllakk slagvvclva valgacvatg vhhylsafep lpythisdid
      541 lffkghaamy lwptghltsf vagvlvafcf hkyshvrfgk vtyavcwlls vagglavvys
      601 vfdwnhrgay vqawastyag lqrlvwatav cwvvfvcttg qavlvnrlls mdvfvplsrl
      661 tycgylvqml vistrlvtlr hsvyythwsm vrdylghtvl ismfaylmyl lcecptvkls
      721 kmalsgltrt fsrggktgsq dsvnqnccrl
//
LOCUS       XP_077506869             398 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116081 [Amblyomma americanum].
ACCESSION   XP_077506869
VERSION     XP_077506869.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650743.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..398
                     /product="uncharacterized protein LOC144116081"
                     /calculated_mol_wt=45517
     Region          182..343
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..398
                     /gene="LOC144116081"
                     /coded_by="XM_077650743.1:28..1224"
                     /db_xref="GeneID:144116081"
ORIGIN      
        1 msvvlsssvd lalsrrkvre irrrllanna llialassrr tvrrqvwayp rnerwfedtl
       61 pglgsrhfrq sfrvsetsfr ylvdvcrpam erqitnmrep ipvekrvava syklcscaed
      121 ravadvfgig rstvnelyre fcetvvttle hewvkmptav dmrahirefe amcgfpqavg
      181 aldgchiavs ppkehasdyy nykgwysvil lalvdhkyhf rfvnagspgr chdsyvyqns
      241 mlarmvespi fsaptatinr vivpplilcd qafpltpnlv kpfrsldtcd ghrsynyhls
      301 kcrrivenaf grlkarfrli skrmdanvkn vaqiiraccv lhnicehfnd svaqqwlhev
      361 eiddavhvqp ahssdvvtan gadvrealve yyrqfeqh
//
LOCUS       XP_077506901             157 aa            linear   INV 25-NOV-2025
DEFINITION  histone H2A type 2-C-like [Amblyomma americanum].
ACCESSION   XP_077506901
VERSION     XP_077506901.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650775.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..157
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..157
                     /product="histone H2A type 2-C-like"
                     /calculated_mol_wt=16754
     Region          2..78
                     /region_name="HFD_H2A"
                     /note="histone-fold domain found in histone H2A and
                     similar proteins; cd00074"
                     /db_xref="CDD:467020"
     Site            order(2..3,14..15,18,22,63,65..67,70)
                     /site_type="other"
                     /note="Hif1 binding site [polypeptide binding]"
                     /db_xref="CDD:467020"
     Site            order(2..3,6,14..15,18,28..31,60..61,63)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:467020"
     Site            order(3..5,10..12,15..16,18..20,28,30,32..33,35..50,
                     52..54,56,61,63..66,69,73,78)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467020"
     Site            order(24,27)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:467020"
     CDS             1..157
                     /gene="LOC144116097"
                     /coded_by="XM_077650775.1:1..474"
                     /db_xref="GeneID:144116097"
ORIGIN      
        1 msrsaraglr lpvgrihrlm cngkyaerig staavymaav leyltaellq lasngardna
       61 ktrimpchiq lairnhddlr klrrklrrea segngamtlg agdamapggg gdamtpgggv
      121 lpyipaehpp kergtsssqk crkdgksgag rqdylwi
//
LOCUS       XP_077506919             215 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116112 [Amblyomma americanum].
ACCESSION   XP_077506919
VERSION     XP_077506919.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650793.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..215
                     /product="uncharacterized protein LOC144116112"
                     /calculated_mol_wt=23200
     CDS             1..215
                     /gene="LOC144116112"
                     /coded_by="XM_077650793.1:257..904"
                     /db_xref="GeneID:144116112"
ORIGIN      
        1 mydrvssgpl prltticahp mtscssltsg qcrysngtlp tcslqqrsle dslaveaycl
       61 tpfiqaarie easlgpwvse aaggvcslia vavlqmkfre letlsvalhl tafvllvdpr
      121 ssldailidv atmhvidlfp sgstakypll atlhfhpmts cssltsgqcr ysngtlptcs
      181 lqqrsledcl aveaycltpf iqasriecvn krfel
//
LOCUS       XP_077506940             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116124 [Amblyomma americanum].
ACCESSION   XP_077506940
VERSION     XP_077506940.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650814.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 25% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144116124"
                     /calculated_mol_wt=13772
     CDS             1..127
                     /gene="LOC144116124"
                     /coded_by="XM_077650814.1:287..670"
                     /db_xref="GeneID:144116124"
ORIGIN      
        1 mpdfsevtal qeqlyqalas dlrgraevlr vmwsaerres sdararasta eaglqvaatp
       61 vpqptfgevl rgpgsrpavs gsasaepsti nqrptvapqh rpwerhsrtl rclrdkdgii
      121 ltlklnt
//
LOCUS       XP_077506950             196 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116127 [Amblyomma americanum].
ACCESSION   XP_077506950
VERSION     XP_077506950.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650824.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..196
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..196
                     /product="uncharacterized protein LOC144116127"
                     /calculated_mol_wt=21168
     CDS             1..196
                     /gene="LOC144116127"
                     /coded_by="XM_077650824.1:1..591"
                     /db_xref="GeneID:144116127"
ORIGIN      
        1 myivqnvsds rdaiasaktg sgvssvflll klpalvtana rgssresqhq qpfqgvhmle
       61 attsrlksiv qiekalseka cfwmqgkdap lldtahisgm ccqigtpsva prgygrqwml
      121 pitvpevipv lprepvlssv flsvgilrga dfvvllpsfy vpefekrmni racwdsdvfv
      181 aimgetaagm lalfla
//
LOCUS       XP_077506962             216 aa            linear   INV 25-NOV-2025
DEFINITION  putative ATP-dependent RNA helicase DDX4 [Amblyomma americanum].
ACCESSION   XP_077506962
VERSION     XP_077506962.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650836.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..216
                     /product="putative ATP-dependent RNA helicase DDX4"
                     /calculated_mol_wt=23408
     Region          <82..148
                     /region_name="DEAD-like_helicase_N"
                     /note="N-terminal helicase domain of the DEAD-box helicase
                     superfamily; cl28899"
                     /db_xref="CDD:475120"
     CDS             1..216
                     /gene="LOC144116134"
                     /coded_by="XM_077650836.1:1..651"
                     /db_xref="GeneID:144116134"
ORIGIN      
        1 mtcvplglat edhwgasyie tspiemyvvs yllarrdfda rartnsstla vamqlmleal
       61 ekgsapgtsr ecqhrppphg lrtsdllgne evssekacff vlgkgdslld mafeprvsfq
      121 vrrpsvprrc srrplmssvi vqeliralpp eptlncavls tgilggagsd vqppllfvtq
      181 seehlkrvdv lacrdsevvv aimnemaagt lafivp
//
LOCUS       XP_077506996             355 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116162 [Amblyomma americanum].
ACCESSION   XP_077506996
VERSION     XP_077506996.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650870.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..355
                     /product="uncharacterized protein LOC144116162"
                     /calculated_mol_wt=39458
     Region          <70..172
                     /region_name="Ty3_capsid"
                     /note="Ty3 transposon capsid-like protein; pfam19259"
                     /db_xref="CDD:437091"
     Region          <215..>276
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     Region          304..>352
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(311,313,315,341..343)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133137"
     Site            311..313
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133137"
     Site            311
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133137"
     Site            order(341..344,347..350)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133137"
     CDS             1..355
                     /gene="LOC144116162"
                     /coded_by="XM_077650870.1:1..1068"
                     /db_xref="GeneID:144116162"
ORIGIN      
        1 maqalgaqpl faappqfdsn aetwtnyser lesyfdanei tddarkrail itclnpevyg
       61 rlrsllapkk prdepyeaii allkqhlnpa pseiyktyrf qtrvqktgek vgeylaelrk
      121 iadncgfgsa lernlrdrfv iglrekmvqr vllakpkslt lqeavdiats telaiqnver
      181 lphastaesa nevnatafkk ktnpqyrhtk aktprpcnrc gdcshdapns phktstcykc
      241 hkqghlatrc fggvkakrpt kfqanavsge agdssctpvl diytvtsegt avepvyktvk
      301 wgpvnldmqv dtgspvciip eeiyyfhakq wpalkptnke lhcylgklpi sgcfi
//
LOCUS       XP_077507005             795 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116171 [Amblyomma americanum].
ACCESSION   XP_077507005
VERSION     XP_077507005.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650879.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 14% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..795
                     /product="uncharacterized protein LOC144116171"
                     /calculated_mol_wt=85492
     Region          <9..563
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <479..>774
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..795
                     /gene="LOC144116171"
                     /coded_by="XM_077650879.1:1..2388"
                     /db_xref="GeneID:144116171"
ORIGIN      
        1 mqqqqptsrs fklqkqeqqa tanskdqqet srssskkqpa sskhqqtass skqkaviisn
       61 kqqqtasskk qqqtasimqp kqaastkqqq qtarcstqqq easrkqqqva ssskkqtear
      121 skqqqtasss kkqaaakskh qatcsrskqq valrsisskq qqaassskhq eaatnskhha
      181 aearskqqaa gskqpktast kqhaaeensk qhqeaaaasr kkqeqrastm qqkqaastrs
      241 khqgaaansr qqkvasskqp paaaskgkqq tastrtkatn skqqqseatr kpeaaarskq
      301 qeapiskqqq tassskqqns issskhaass srqlqesssi kqqapcsrsk qqapaastmq
      361 qkqaastssk hhaaeastqq qtassrkkka arnskkqaan ssrskqqval rsisskqqqa
      421 assskhqeaa tnskhhaaea rskqqaagsk qpktastkqh aaeenskqhq eaaaasrekq
      481 eqrstkqqta gnrkqqqtan rsknqaekrs klqaaartkp qaagrtskkq qqaterkkqq
      541 aaankkqeas lkqqqeasss nqqgassrkq qhsassckkq aasnihqqaa vaatssskhq
      601 aawnskkhaa srckqqaavr sthqqqapcs ssnqqaaass crskkqtaas kskqqaaais
      661 skkeagsrsk khaansskkq vanssrrqqe assssknqaa prtkkkeats kqqsapsskr
      721 qqtaisskqe aesrnqqqaa ristkhqepa agsssskhha aeasrmqqqq aeaacsskkq
      781 aasskeqlet srkqq
//
LOCUS       XP_077507017             410 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116178 [Amblyomma americanum].
ACCESSION   XP_077507017
VERSION     XP_077507017.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650891.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 15% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..410
                     /product="uncharacterized protein LOC144116178"
                     /calculated_mol_wt=43164
     Region          185..>349
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     CDS             1..410
                     /gene="LOC144116178"
                     /coded_by="XM_077650891.1:1..1233"
                     /db_xref="GeneID:144116178"
ORIGIN      
        1 mtkklqnrda trsaeqsqpr kltrrssaca ptaaaavvtr damaasyvwa rrrrklrrre
       61 assnkskqqp aassskkqea rtsiqqaatr kqktganske qqaalsckpq qptpptnkqq
      121 apcsrsskeq taaasskqqq aaqaaissnk qaaarskpqa aarskqqaaa rtkqqtgsss
      181 elhaaaigkq qgpafsnqqq aaarskqqaa tssskkqsaa sskhhgaeas nsskqqaans
      241 khqtaaassk hhasrkqqqe gsskqqraaa rikqqqaast ssthhaaeas tkqqttsssr
      301 nkaasssknq aassskkqaa sssnqqgans sklqqepsrk qqqeasrkqk qaassskkqa
      361 eisnqkqaas tskqeaasss kqqltanskh haaaatrkqq lqaaaasssk
//
LOCUS       XP_077507030             273 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116181 [Amblyomma americanum].
ACCESSION   XP_077507030
VERSION     XP_077507030.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650904.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 41% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..273
                     /product="uncharacterized protein LOC144116181"
                     /calculated_mol_wt=29711
     CDS             1..273
                     /gene="LOC144116181"
                     /coded_by="XM_077650904.1:1..822"
                     /db_xref="GeneID:144116181"
ORIGIN      
        1 mpvrsagtlp lavarhlrap alhevimrvg ahqkkqrtkk rsgarerrds shrmrtradt
       61 scaaelpsed lrgskptrll vtfmagqlqc nlrlhnirks vqraarmvgn dvtrspfefl
      121 slvplwpkql vhsadvpdia vfisaagvta cftrqaclgs pmctpligsm lcpfkrklce
      181 llggwlstic dtlsplasga fclsfqfahm aaecackavl lylewfivas flvsslcrir
      241 hflsatvtak kpaashqgss kskkngtgaq llk
//
LOCUS       XP_077507049             238 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116193 [Amblyomma americanum].
ACCESSION   XP_077507049
VERSION     XP_077507049.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650923.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..238
                     /product="uncharacterized protein LOC144116193"
                     /calculated_mol_wt=26743
     Region          59..>152
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     CDS             1..238
                     /gene="LOC144116193"
                     /coded_by="XM_077650923.1:2..718"
                     /db_xref="GeneID:144116193"
ORIGIN      
        1 mqvgvpytlf akkssnyclv qlpspqccfc lavecvnvir allllsgdve tnpgppntda
       61 vlaelqklsa gqrklisevq glknqlvttd ktisalseri anietqfevi stlrteietl
      121 qtstartthq lseidtrldd lenrsrrnnl vfyglpdpnd kesydqleki iiqhcldsln
      181 pvidpkdier ahrlgrhsid rqrpiivkls afktkppkty nseavcfqnq rghsfewp
//
LOCUS       XP_077507074             239 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116216 [Amblyomma americanum].
ACCESSION   XP_077507074
VERSION     XP_077507074.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650948.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..239
                     /product="uncharacterized protein LOC144116216"
                     /calculated_mol_wt=26738
     CDS             1..239
                     /gene="LOC144116216"
                     /coded_by="XM_077650948.1:1..720"
                     /db_xref="GeneID:144116216"
ORIGIN      
        1 mfaqveavfd lrlitsqcvk ylhvvstlsp eemdefgdvl ktpdpsspcd hfkvtvldrk
       61 iisershlqq lpstedlgdr qpsqllhcmq qllgdrpqnv ssplhrelfl qrqdlvvvla
      121 aagdvsldkl velahrvshy sgprsvavip siasstlvdq qssleakiyh ltstvaalra
      181 siprrfqrss dqrlrhrsss shrsspardg fcwyhckfna aarqrrqhcl wsgnapash
//
LOCUS       XP_077507081             232 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116224 [Amblyomma americanum].
ACCESSION   XP_077507081
VERSION     XP_077507081.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650955.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 11% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..232
                     /product="uncharacterized protein LOC144116224"
                     /calculated_mol_wt=25773
     Region          33..109
                     /region_name="DUF4806"
                     /note="Domain of unknown function (DUF4806); pfam16064"
                     /db_xref="CDD:465001"
     CDS             1..232
                     /gene="LOC144116224"
                     /coded_by="XM_077650955.1:1..699"
                     /db_xref="GeneID:144116224"
ORIGIN      
        1 mvatltkskl drqlavlski aaeviceaqs adtvapddmp iypvktqeel dilepstkda
       61 atfatlvktl gqiggtdirg ttrrvfrslv sddiavqynw terkgnkrag klaiirlvfa
      121 avrvtrsaid demaraladw lrfsnsrlta akasnsltfa cilmwqykaa vfrskhvvml
      181 tgrnsleysy mgqffrflqa pgmqispgif fsqekllcic rvitenhelk ai
//
LOCUS       XP_077507087             379 aa            linear   INV 25-NOV-2025
DEFINITION  alanine--glyoxylate aminotransferase 2, mitochondrial-like
            [Amblyomma americanum].
ACCESSION   XP_077507087
VERSION     XP_077507087.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650961.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 16% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..379
                     /product="alanine--glyoxylate aminotransferase 2,
                     mitochondrial-like"
                     /calculated_mol_wt=40495
     Region          <42..60
                     /region_name="transpos_IS481"
                     /note="IS481 family transposase; NF033577"
                     /db_xref="CDD:468094"
     Region          <64..375
                     /region_name="ArgD"
                     /note="Acetylornithine aminotransferase/4-aminobutyrate
                     aminotransferase [Amino acid transport and metabolism];
                     COG0160"
                     /db_xref="CDD:439930"
     CDS             1..379
                     /gene="LOC144116234"
                     /coded_by="XM_077650961.1:1..1140"
                     /db_xref="GeneID:144116234"
ORIGIN      
        1 mfvtvdcdit gthgiaetnm slllgqqevm rlsgtsyska tayhpqtngl terlnktiad
       61 tismnayhgm spycaslcgv gnykhhipss fgtfhamnpd pyrgvwggak crdspvqtdr
      121 tcscgtecea aslyadqlse vlqqsvskkk vaaffaesiq gvggsvqypk gylkkahkli
      181 kdkgglfvad evqtgfgrtg dhywgfeghg impdivtmak gigngfplaa vittpeiska
      241 ldaasffntf ggnpvatavg savvevldde gcmenarvmg nrllqalesl rkefpvigdv
      301 rgkglmlgve lvenketkvp lgaekvnail ehcremgvli gkggqyghvl rikppmcite
      361 advdftaavl nraiahvqq
//
LOCUS       XP_077507092             513 aa            linear   INV 25-NOV-2025
DEFINITION  DNA-dependent metalloprotease dvc-1-like [Amblyomma americanum].
ACCESSION   XP_077507092
VERSION     XP_077507092.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650966.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 20% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..513
                     /product="DNA-dependent metalloprotease dvc-1-like"
                     /calculated_mol_wt=56977
     Region          64..232
                     /region_name="SprT"
                     /note="SprT homologues; smart00731"
                     /db_xref="CDD:214794"
     CDS             1..513
                     /gene="LOC144116246"
                     /coded_by="XM_077650966.1:199..1740"
                     /db_xref="GeneID:144116246"
ORIGIN      
        1 msmasedeee lalemaeeha yhvsllelne psieepaats vathslrkdl svvdpfwqqc
       61 dpypdvqrlf vefneayfyg kldqvavswn whmtlcaglc eyegdglctv klsepllklr
      121 trkdlvetll hemihaylfv tdgnmdhdah gpefhkhmdr vnkasganit vfhnfheeln
      181 nyrthwwrcn grcqhrfpff givrramnrp psdydrwwad hqrncggtft kirepdpkgs
      241 kgkrkdgtlp vkndakqpkl mdgadirapl vpphsvkstv svpdsqshqg trpkhvssfp
      301 ntmtklpsnv htlpqhatdl qwpqvvpftg egrrlggcss ksrllgesnc vlggvssqml
      361 hidaneatsw ssavsgpgqs lskcvtetqv kpapqkpsds twptlkdfld qeigsdifss
      421 ntesdddcvi teadmtvkeq gkctgkldea sassssslts tdvgatanev mrlsgtsysk
      481 atayhpqtng lterlnktia dtismyadad hrn
//
LOCUS       XP_077507102             122 aa            linear   INV 25-NOV-2025
DEFINITION  sentrin-specific protease 1-like [Amblyomma americanum].
ACCESSION   XP_077507102
VERSION     XP_077507102.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650976.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..122
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..122
                     /product="sentrin-specific protease 1-like"
                     /calculated_mol_wt=14395
     Region          <11..118
                     /region_name="Peptidase_C48"
                     /note="Ulp1 protease family, C-terminal catalytic domain;
                     cl23802"
                     /db_xref="CDD:420019"
     CDS             1..122
                     /gene="LOC144116256"
                     /coded_by="XM_077650976.1:1..369"
                     /db_xref="GeneID:144116256"
ORIGIN      
        1 mvpvrsqdas hwwlavvdfr tpemviydsl gpcqkcaesi dalaqyleev shlpdcdldw
       61 sgwhfypriv plqkntsdsa vfmccytecl trevqilfqq khmpyfrrri vyeilhrrll
      121 sr
//
LOCUS       XP_077507161             267 aa            linear   INV 25-NOV-2025
DEFINITION  sentrin-specific protease 1-like [Amblyomma americanum].
ACCESSION   XP_077507161
VERSION     XP_077507161.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651035.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..267
                     /product="sentrin-specific protease 1-like"
                     /calculated_mol_wt=30650
     Region          <15..226
                     /region_name="Peptidase_C48"
                     /note="Ulp1 protease family, C-terminal catalytic domain;
                     cl23802"
                     /db_xref="CDD:420019"
     CDS             1..267
                     /gene="LOC144116292"
                     /coded_by="XM_077651035.1:1..804"
                     /db_xref="GeneID:144116292"
ORIGIN      
        1 maaisiegdv nhhrpltsam qeevsealkw sppeqvvveg fsmaltrrdi atliegkrln
       61 dnivkfylam iaerserrgg ppvyafssff ftklyrcgpg gakrwtqgad lfsydnllvp
      121 vrsqdashww lavvdfrtpe mviydslgpr qkcaesidal aqyleevshl pdcdldwsgw
      181 hfcprivplq kntsdsavfm crytecltre vqilfeqkhm pffrrricvr dftpqapfmv
      241 kptcnnmsrf kcpciqymaf gwlyacs
//
LOCUS       XP_077507188             248 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116318 [Amblyomma americanum].
ACCESSION   XP_077507188
VERSION     XP_077507188.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651062.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..248
                     /product="uncharacterized protein LOC144116318"
                     /calculated_mol_wt=27615
     CDS             1..248
                     /gene="LOC144116318"
                     /coded_by="XM_077651062.1:1..747"
                     /db_xref="GeneID:144116318"
ORIGIN      
        1 matrgfpnqi pefngsswss wvgrlqfyfe anitdpalkr anlltlcgeq tydtvcaliq
       61 pstpaavsyd divaalqkhy dprpsevysr arfqrrdqle getvsayiaa lkklaahcnf
      121 gtltttatgq erdaassant tmlpldvmlr drfvcglsde slqqrmfaek dltfnkaydi
      181 avraesadhq qgqirrntep eerdghlpal dgvdlrlglq rallvdagep rraaapwrra
      241 erqpqqev
//
LOCUS       XP_077507215             272 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116338 [Amblyomma americanum].
ACCESSION   XP_077507215
VERSION     XP_077507215.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651089.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 23% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..272
                     /product="uncharacterized protein LOC144116338"
                     /calculated_mol_wt=30030
     Region          143..163
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          174..196
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          174..194
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(179,181,183,185..186,189..190,193,207,209,213..214,
                     217..218,221,235,237,239,241..242)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          186..211
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          202..222
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..272
                     /gene="LOC144116338"
                     /coded_by="XM_077651089.1:1..819"
                     /db_xref="GeneID:144116338"
ORIGIN      
        1 mdpgvffgkr pfcrlseale vaadvavhae epvdivivpp epsvdtdvee adddgttdtn
       61 sqpeyhwatp trgtkereln lkslffgksp nevfstildd evlthivvht gkyakenneh
      121 gfviniedeg gnsevrpkcv fscgacgelf saqdllekhr rrkhpqrpeg rhccahcpys
      181 sdrkpnvvrh vrthtgwrpf lcqvcgkgfa rldhltghql vhsgeglhec pecgqrfsqa
      241 snqtgcspqg aalcgrrgcl apvyplrevv qp
//
LOCUS       XP_077507223             314 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin-like domain-containing C-terminal domain phosphatase 1
            [Amblyomma americanum].
ACCESSION   XP_077507223
VERSION     XP_077507223.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651097.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..314
                     /product="ubiquitin-like domain-containing C-terminal
                     domain phosphatase 1"
                     /calculated_mol_wt=36208
     Region          4..76
                     /region_name="Ubl1_cv_Nsp3_N-like"
                     /note="first ubiquitin-like (Ubl) domain located at the
                     N-terminus of coronavirus SARS-CoV non-structural protein
                     3 (Nsp3) and related proteins; cl28922"
                     /db_xref="CDD:475130"
     Region          111..302
                     /region_name="HAD_like"
                     /note="Haloacid Dehalogenase-like Hydrolases; cl21460"
                     /db_xref="CDD:473868"
     Site            order(137..141,177..178,224,245..246,249..250)
                     /site_type="active"
                     /db_xref="CDD:319823"
     CDS             1..314
                     /gene="Ublcp1"
                     /coded_by="XM_077651097.1:1..945"
                     /db_xref="GeneID:144116348"
ORIGIN      
        1 msflcitvqf agkeyelsdl pldgcmmdlk naifektglr peqqilggik weakplseev
       61 rlselkfdst aknmmvwsle synvpedipd vmndlnnded kdmpienpdd eiakliktyk
      121 ikwlnesrtg krllvldidy tiiakwtttk smeelmrpfl heflesayqd ydiafwsatk
      181 mmwidaklwq lgvtrnekyk iaflldsgam islhtskygl vnvkplpliw glvsayrpen
      241 timiddiprn flmnpqsglr vrpfrnadph qnndrelvhl arylkdiaas vsdftelnhr
      301 nwktsaeddv lgst
//
LOCUS       XP_077507237             321 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116352 [Amblyomma americanum].
ACCESSION   XP_077507237
VERSION     XP_077507237.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651111.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 36% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..321
                     /product="uncharacterized protein LOC144116352"
                     /calculated_mol_wt=36363
     Region          <1..64
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     CDS             1..321
                     /gene="LOC144116352"
                     /coded_by="XM_077651111.1:1..966"
                     /db_xref="GeneID:144116352"
ORIGIN      
        1 meellknvpg tqvflddvli aedknsygkt vnkvleifre ngirlrkdkc vfgaqevtyl
       61 ghkidrhglh paekkldtiv rapaprnvqe hrsflglity yrsflpnmst alaspddepe
      121 lwssrktnff lnkykemdfv gktrvlrtri qlwlklceli nkkvecnmsp tqvenkwksl
      181 dlaykkskna dsssghhrvs ceheeelati lekehsvnpr lllepgkail psgspsttgg
      241 gppedlpvpv eggvtpkrir hnrnqlgpll dglqkmadak tkalearkkw edarakrhdd
      301 lmkrfdrlid nlakqqgsna q
//
LOCUS       XP_077507274             467 aa            linear   INV 25-NOV-2025
DEFINITION  sodium-coupled monocarboxylate transporter 1-like [Amblyomma
            americanum].
ACCESSION   XP_077507274
VERSION     XP_077507274.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651148.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..467
                     /product="sodium-coupled monocarboxylate transporter
                     1-like"
                     /calculated_mol_wt=50706
     Region          1..422
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     Site            order(45,48,331,334..335)
                     /site_type="other"
                     /note="Na binding site [ion binding]"
                     /db_xref="CDD:271356"
     CDS             1..467
                     /gene="LOC144116375"
                     /coded_by="XM_077651148.1:14..1417"
                     /db_xref="GeneID:144116375"
ORIGIN      
        1 maanlglglr fsfrrgtsap dstagevflg srtlrslpla lsvvasmvss tgligftahl
       61 yafgfhilwt lvplvavlpl atelvvpvfy tlritsvfqy lrmrfnscis ltacvsyval
      121 tqsvgavvif aasiaiatvf svpltwcslg iglcgtlytt lggmrgvvwt dcmqvlfill
      181 apatvigkvi ydarsglarl rpwddldfha yaldfrldig ndenvwstll attainiyrt
      241 sfdqvvvqry iasrtlkear rtavvglvlt tfmylaeaam alaltlwfrg cdpesmgqiq
      301 kldqilpfyv ktylvqfpgt vglflsgivs aatstissli nsqaailyvd vvspqlpise
      361 vhvaritkci afvvgvvmti ystlvpymgs itrifmmana aisgplaglm lmavifpfvn
      421 akvrigtlpl fhlslysktr rfrselliam lclsepvsra pcsnltr
//
LOCUS       XP_077507279             187 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116384 [Amblyomma americanum].
ACCESSION   XP_077507279
VERSION     XP_077507279.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651153.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="uncharacterized protein LOC144116384"
                     /calculated_mol_wt=20274
     CDS             1..187
                     /gene="LOC144116384"
                     /coded_by="XM_077651153.1:138..701"
                     /db_xref="GeneID:144116384"
ORIGIN      
        1 maerllrnhr lhnirksvqr aapilgndvt rspfeflslv plgpkplvhc adipdtavfv
       61 saagvtacft rqacleprmc tplfvsilcp fkrslcellg gwlgticdil splaagvfhl
      121 sfqfarmvge cackavllyi ewfiaasflv pllswtrhfl fatvtgkkpa asdqdsskgn
      181 kngtgaq
//
LOCUS       XP_077507287             230 aa            linear   INV 25-NOV-2025
DEFINITION  collagen alpha-1(V) chain-like [Amblyomma americanum].
ACCESSION   XP_077507287
VERSION     XP_077507287.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651161.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..230
                     /product="collagen alpha-1(V) chain-like"
                     /calculated_mol_wt=25841
     Region          34..>201
                     /region_name="COLFI"
                     /note="Fibrillar collagen C-terminal domain; cl02436"
                     /db_xref="CDD:470578"
     CDS             1..230
                     /gene="LOC144116394"
                     /coded_by="XM_077651161.1:1..693"
                     /db_xref="GeneID:144116394"
ORIGIN      
        1 mtrvtlcrfq gplgegaqip peillqrdlh nrftlyilrp eieqirrllg srpnpsrscr
       61 dpwlahpdss dgwhwvdpnw gvpddavrvf cgmraggetc vfvdgdtdsa rlssnasswf
      121 sslkggfrlr nrtvgdvqlr flsllstgey rnftfvcnnv nawysqrtkd haralrfrgr
      181 ndaqmsfgkd scrvpvndcq tiavvslipa lrsvaqnvsa nrrsenlsdi
//
LOCUS       XP_077507291             267 aa            linear   INV 25-NOV-2025
DEFINITION  collagen alpha-1(V) chain-like [Amblyomma americanum].
ACCESSION   XP_077507291
VERSION     XP_077507291.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651165.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..267
                     /product="collagen alpha-1(V) chain-like"
                     /calculated_mol_wt=30020
     Region          87..>264
                     /region_name="COLFI"
                     /note="Fibrillar collagen C-terminal domain; cl02436"
                     /db_xref="CDD:470578"
     CDS             1..267
                     /gene="LOC144116410"
                     /coded_by="XM_077651165.1:1..804"
                     /db_xref="GeneID:144116410"
ORIGIN      
        1 mttvqlcrlq gppgegpqnp pemlfqrdlh nrftrslekv dnacteedya vvtgddgndd
       61 ddkistaave pvdelayeki srtlthifak vyilrpeieq irrpvgsrpn qsrscrdlwl
      121 ahpdstdgwy wvdpnwgvpd davrvfcgmr aggetsvlad gvtdsarlsw wpasasaasn
      181 asswfsrqkg gfrlryrtvg dvqlrflsll stgeyrnftf vcnnvnawys qrtkdharal
      241 rfrgrneaqm sfakdsptvp vddcqgy
//
LOCUS       XP_077507322            1021 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116469 [Amblyomma americanum].
ACCESSION   XP_077507322
VERSION     XP_077507322.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651196.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 20% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1021
                     /product="uncharacterized protein LOC144116469"
                     /calculated_mol_wt=116651
     Region          10..>158
                     /region_name="COLFI"
                     /note="Fibrillar collagen C-terminal domain; cl02436"
                     /db_xref="CDD:470578"
     Region          185..273
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          454..639
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..1021
                     /gene="LOC144116469"
                     /coded_by="XM_077651196.1:1..3066"
                     /db_xref="GeneID:144116469"
ORIGIN      
        1 mtrgdnvyil rpeieqirrl lgsrpnpsrs crdpwlahpd ssdgwhwvdp nwgvpddavr
       61 vfcgmragge tcvfvdgdtd sarlssnass wfsslkggfr lrnrtvgdvq lrflsllstg
      121 eyrkftfvcn nvnawysqrt kdharalrfr grndaqmsfg kdscrvsvkd cqndphhgpr
      181 lnvhkncrgg ydngpkvrlf sfpsdptrks lwqravrrdd vdvstlkdpk vcerhfkpeh
      241 lrttstytdv dgrtievpmk itritsdavp tlfpdcpsyl satrqsreep evkrrrnena
      301 qlqeaiqqsa aafemeeamn kiqsledisa rlqrlhqkdy wtnvccdkci ifahlepttq
      361 apellasvtv sadmcvrvfw khvqlasnee leipgqvldf rvlerlldnv qgyctdrshq
      421 kqdrvsaalk livallddis ddeitehera dalfflkeqf elmgkknngc rysaellvls
      481 avfhtispha ynflrssgkv vlphattikr vcaaqdvsps keqteegflr ymkrraglln
      541 phernivlmm deihlqqffe ykggrftgaa ancaepakta hvfmvqslls sykdvahilp
      601 vsritaeelh rvlrgvimnl edaglhvvav itdnnsinrk vmslfgrnhk plivyphpaq
      661 qqlplfhiid tvhllkcirn nwlnqktcdk clyfppfese qeqgvkmqga sftslkklye
      721 aeqdsvvkva ygltykslnp tnlerqnvkl amkifssfvs salrlrgtel klilpeetae
      781 fidlvlrwwn ivnvktpqkg wrlrdpwqep isamsckqie fldkfatwld nwksrgmqtg
      841 tltnethsal rqstyaliev arycldelgf kyvllgkfqt nalearfgky rrlcgshynv
      901 sitevfeaet kiriqntlll kdftdsskee nepepcpeal ikkykvclsd aeikkprkdm
      961 paivyiagfc ahaalrrqpc ddcllqltir erelqpsdhv lindmsrggl kfpqpfvvht
     1021 v
//
LOCUS       XP_077507329             119 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116499 [Amblyomma americanum].
ACCESSION   XP_077507329
VERSION     XP_077507329.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651203.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 22% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..119
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..119
                     /product="uncharacterized protein LOC144116499"
                     /calculated_mol_wt=12884
     CDS             1..119
                     /gene="LOC144116499"
                     /coded_by="XM_077651203.1:165..524"
                     /db_xref="GeneID:144116499"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrppfa altsvlccqr pasigpcvlp afgcvippdp
       61 fmlktkscme vvqatklrvi fsrdleqagk lvdgqwysls aahvfkmcgw gvgrtishf
//
LOCUS       XP_077507332             461 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116506 [Amblyomma americanum].
ACCESSION   XP_077507332
VERSION     XP_077507332.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651206.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..461
                     /product="uncharacterized protein LOC144116506"
                     /calculated_mol_wt=50902
     Region          <122..455
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..461
                     /gene="LOC144116506"
                     /coded_by="XM_077651206.1:1..1386"
                     /db_xref="GeneID:144116506"
ORIGIN      
        1 mniekhvlsi ldavrdtriq arpavsslrr iaapvreatl pnwleplqaa fwwgsfdent
       61 svsvqdvrvl laadqlllsh ssldlleqls wwlvqrltal gwpqgyiava ggkevanhtv
      121 kedcyslaat rfglllases avklftrdsr rqaqaflrdl ltvfrelaas aswlsekara
      181 vvnktlqelq ivlwppdvai skaslwqlys nfcftcetay qaavdggaqa elapdgrpta
      241 mdhwmhanra fsrldpslre rmqilwqgga aepfvfehwk nrlrvshaav gtplyhvtsa
      301 egggeeanyg glgaaflshv lrsfdppaen iddnlvpwie isgaaykags lkcgadfipn
      361 lreiaalgia whalkkrraa avkgsdsdgr rqlpatalsi pdsngevhvy tgdqlfflnh
      421 crsrcrgqlp rdsrrgedcn vavrhvrdfg ptfgcatrrg w
//
LOCUS       XP_077507335             198 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116543 [Amblyomma americanum].
ACCESSION   XP_077507335
VERSION     XP_077507335.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651209.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 27% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..198
                     /product="uncharacterized protein LOC144116543"
                     /calculated_mol_wt=20515
     CDS             1..198
                     /gene="LOC144116543"
                     /coded_by="XM_077651209.1:1..597"
                     /db_xref="GeneID:144116543"
ORIGIN      
        1 messkpppvs tlpavflfld ekdstnsatv wkelaleggf lrtergascv wktarpvlls
       61 hpeqattlll llalavcnts gqgqgkcvgr gqgqghgqgr gkhgggpavg gplvadgrpl
      121 laggggqfre sggfrqthgh rveegfeqtd fynqgrgnga aflgtnsgvl qsgllqevkg
      181 kfkimgkvrg ytassalq
//
LOCUS       XP_077507336             111 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116559 [Amblyomma americanum].
ACCESSION   XP_077507336
VERSION     XP_077507336.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651210.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 40% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..111
                     /product="uncharacterized protein LOC144116559"
                     /calculated_mol_wt=12052
     CDS             1..111
                     /gene="LOC144116559"
                     /coded_by="XM_077651210.1:1..336"
                     /db_xref="GeneID:144116559"
ORIGIN      
        1 mtlpteswvc acfggapppt stltgplrgg wsrhskhsst lyrelvrgsq rhqskhgerr
       61 ldaaywnhtt pqshrtnrwk vaasppaplp gaehsdppel rdllaaamva p
//
LOCUS       XP_077507337             227 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116576 [Amblyomma americanum].
ACCESSION   XP_077507337
VERSION     XP_077507337.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651211.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..227
                     /product="uncharacterized protein LOC144116576"
                     /calculated_mol_wt=25329
     Region          4..97
                     /region_name="THAP"
                     /note="The THAP domain is a putative DNA-binding domain
                     (DBD) and probably also binds a zinc ion; smart00980"
                     /db_xref="CDD:214951"
     CDS             1..227
                     /gene="LOC144116576"
                     /coded_by="XM_077651211.1:108..791"
                     /db_xref="GeneID:144116576"
ORIGIN      
        1 mvncavvgcs nrsdvtgrkk rptsarffsl pkvienqcer tktlsakrrs lwlarikran
       61 fnhespnvrv cgvhfikgep snlfdetnpd wapslslghg trhadsarhd rrakrlahkr
      121 radveatava seaspracpn spqppqaeev sadenetgia vqtdmtmqdt qaleqhsvvl
      181 nehlytlqke keqlevteds lksceakvrl yqglrnffgk ehvpppp
//
LOCUS       XP_077507338             515 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116592 [Amblyomma americanum].
ACCESSION   XP_077507338
VERSION     XP_077507338.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651212.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..515
                     /product="uncharacterized protein LOC144116592"
                     /calculated_mol_wt=57709
     Region          238..436
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(296,335,349,351)
                     /site_type="active"
                     /db_xref="CDD:411747"
     Region          <464..511
                     /region_name="TNG2"
                     /note="Chromatin remodeling protein, contains PhD zinc
                     finger [Chromatin structure and dynamics]; COG5034"
                     /db_xref="CDD:227367"
     CDS             1..515
                     /gene="LOC144116592"
                     /coded_by="XM_077651212.1:1..1548"
                     /db_xref="GeneID:144116592"
ORIGIN      
        1 mkaykalqsh nyftsgwvkh lsakefqdgk vvllgevnhs qrlghkplqv wilckkdgav
       61 itahctcmag ageacshvga clfavetgik mmksrsctqk dnmwlpayve kvqykrlkdi
      121 nftsskgkkr qldsnsfeag pvkerahvpp lsqqertkly gsiqdggivp sifsvlpgyd
      181 ghfqnpvpmh darlrnlyse emlahdldvl vkkandvmps iiiseetvka vealtrqqss
      241 ssnwflyrag rvtasvmkrv chtsidnpsi sllklicype kqslktpait wgvknehraf
      301 rayqtseaek hdmfkcsksg lhlstrypfv gatpdglvcc accgkavvel kcpfslrevk
      361 dvtematags cletvngvvq lrrqhgyfyq vqtqmavcdv qwcdfvvwtp nllhveriqk
      421 drhfceeila agrkffihai lpelfstyft rqhggpvvns psdvycfcrg petgkmvacd
      481 naschykwfh fscvalkrap kttlwfcpec kgpkg
//
LOCUS       XP_077507340             215 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116624 [Amblyomma americanum].
ACCESSION   XP_077507340
VERSION     XP_077507340.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651214.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..215
                     /product="uncharacterized protein LOC144116624"
                     /calculated_mol_wt=22898
     Region          <126..171
                     /region_name="PHA02030"
                     /note="hypothetical protein"
                     /db_xref="CDD:222843"
     CDS             1..215
                     /gene="LOC144116624"
                     /coded_by="XM_077651214.1:1..648"
                     /db_xref="GeneID:144116624"
ORIGIN      
        1 mrlgwslplv stcaalvcwv aaapqqlgdn pllerqaept pqpprapqpp pgwfnfpgft
       61 vvvpsfqvpn fqvavvnwtf pgfqmpqgpw mpwmpqmpqm pqmpqmpqmp qmpqfnftlp
      121 gfgsfpslps lpglpsvpam pampsfvdls gdipdvpdip dvpdipdvpd vpdviggtga
      181 srtmsvpqdl ppsapissdk ipaassiasd serss
//
LOCUS       XP_077507341             481 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116656 [Amblyomma americanum].
ACCESSION   XP_077507341
VERSION     XP_077507341.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651215.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..481
                     /product="uncharacterized protein LOC144116656"
                     /calculated_mol_wt=53529
     Region          258..354
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..481
                     /gene="LOC144116656"
                     /coded_by="XM_077651215.1:1..1446"
                     /db_xref="GeneID:144116656"
ORIGIN      
        1 mearlqqaas ivdayamdcw lqcapaksel lhvranpkdr taihislsgg pireveelri
       61 lglfihhrlr pdstivklkr vgeqvgrmir rvsnkrgglr grdalrlvya fvtsrilyav
      121 pclrttkqde alidaiirka tkraldlpva tsnaklkalg vlnsyqelre ahivnqytrl
      181 mqtapgrrll nrlhiqhdct peetelipvl wrhmlwvspl prnvdtdmhe nrrqararal
      241 erqhgsrpgv yyvdvggpsp tgfytaavvh qemhvdglsf rapnsmraee vaialaatha
      301 nsriiitdsr kacdhylage isplaacilr rtatdptpkr iiwapghqgv rgneaadaaa
      361 raliyraphp sssgsdtnqp llgfreiith ysdnhrlfpa pakglsktee rtlrrlqtgt
      421 llcpailkhy dpntdgrcph cgetcdifhm vwactqnphl plplplperp tsvvatlakg
      481 l
//
LOCUS       XP_077507342             254 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116734 [Amblyomma americanum].
ACCESSION   XP_077507342
VERSION     XP_077507342.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651216.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..254
                     /product="uncharacterized protein LOC144116734"
                     /calculated_mol_wt=28656
     Region          52..198
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..254
                     /gene="LOC144116734"
                     /coded_by="XM_077651216.1:1..765"
                     /db_xref="GeneID:144116734"
ORIGIN      
        1 maqvdivyre ffaavikvle arwlrmvrre gmaahirefh afsgfphaig amdgchipas
       61 dyyaqrctvv dhrycfryln vgspgrchda ymygrskfcg lvesasfqsp vstiegtvvp
      121 piilcgqafp stsnllkpha nartgtreal fnyqisktri vvngfgrlka rfrytakrme
      181 cklrtakqai raactmhnlc easkdpiegq weqelyefna lyqqpshatd vvtegpevra
      241 alssyfwnqs hqaq
//
LOCUS       XP_077507343             275 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116750 [Amblyomma americanum].
ACCESSION   XP_077507343
VERSION     XP_077507343.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651217.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..275
                     /product="uncharacterized protein LOC144116750"
                     /calculated_mol_wt=31353
     Region          <125..197
                     /region_name="DUF4779"
                     /note="Domain of unknown function (DUF4779); pfam16009"
                     /db_xref="CDD:464971"
     CDS             1..275
                     /gene="LOC144116750"
                     /coded_by="XM_077651217.1:4..831"
                     /db_xref="GeneID:144116750"
ORIGIN      
        1 mchaddatid nvtevvivdn vplltqedva kavsashgyg dyggrhshgh yggghrhhgn
       61 shgrhrgsys graqrhgghr ynryhgnhhg rhhyggyggr hhgseryggy grhggrhagn
      121 hyrnkghrsh gyknvyhkee ygdhktyydd yhegdhrnyy nnhhnyrdhh ggrerygnhh
      181 sgyegrhkhg dsyhrhgggy hsshrgshgn hghqhdrsgh gykrgggsyh rgdqgyhahp
      241 hhrdhyarat nralvyftgh ndqtcareqr rlaap
//
LOCUS       XP_077507344             583 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116766 [Amblyomma americanum].
ACCESSION   XP_077507344
VERSION     XP_077507344.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651218.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..583
                     /product="uncharacterized protein LOC144116766"
                     /calculated_mol_wt=56860
     Region          <61..>305
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <230..>516
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     CDS             1..583
                     /gene="LOC144116766"
                     /coded_by="XM_077651218.1:1..1752"
                     /db_xref="GeneID:144116766"
ORIGIN      
        1 mafaapsiqe wkllvpgcta lssppgkrgk rgkqgdagep gppgasgppg kpgfpgaigi
       61 dgpkgdtgdk gdkgekgdsg idlvsnvkgv krsvkmphgg iadatdfkel anigvtfspg
      121 dvfmmkgsqg eagppgppgp pgppglpgfd ggtgppgpig ppgevgpsgp pglvgpvgkd
      181 glpgpkgekg dkgerglttt ldgntfptgf iegpagppgp pgpagppgrk gdvgipgppg
      241 edgakgdkge rgrrgkkgnt grdgrsirgl pgqkgepgtk gmkgdfglpg hrgekgsvgh
      301 pgrpgedgvp grpgppgdpg akgekgsage tsppglmgmp glpgppgkpg pqgekgdkgd
      361 sapvrymrqk skrrppdhhh hfasdhgggt qgdsafetsy dfvigppgpp glpgtpgfpg
      421 ppgikgdkgi dgqkgepgek gdqghmgpmg lpgpmgpvgp aglpgkegrt gitgppgldg
      481 mkgergapgn kgekgepglp gtdgipglpg pkgdrgetgi lgprgkrgkk gdkgdkgdqg
      541 vpgldapcpl gpdglplpgc gwkppntlgg pvppvppeep lds
//
LOCUS       XP_077507345             150 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116813 [Amblyomma americanum].
ACCESSION   XP_077507345
VERSION     XP_077507345.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651219.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..150
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..150
                     /product="uncharacterized protein LOC144116813"
                     /calculated_mol_wt=16819
     CDS             1..150
                     /gene="LOC144116813"
                     /coded_by="XM_077651219.1:1..453"
                     /db_xref="GeneID:144116813"
ORIGIN      
        1 mnisrtanrt tvhnlssqkl ddaelsvlsl glnfnlgpep dikkmvcave tavknvepsr
       61 rdkartrvvs vlsklnrhqg lnplllkerk alkrlrgnen ivilpadkgn atvvldkrey
      121 vnkmtgmled ertylplnrd ptskvqrdlh
//
LOCUS       XP_077507346             181 aa            linear   INV 25-NOV-2025
DEFINITION  histone-lysine N-methyltransferase PRDM7-like [Amblyomma
            americanum].
ACCESSION   XP_077507346
VERSION     XP_077507346.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651220.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="histone-lysine N-methyltransferase PRDM7-like"
                     /calculated_mol_wt=20596
     Region          113..>169
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     CDS             1..181
                     /gene="LOC144116845"
                     /coded_by="XM_077651220.1:1..546"
                     /db_xref="GeneID:144116845"
ORIGIN      
        1 maseddftal rkyftddewk glsdymktpc snvrenyelm lklasletss srsfyprrqr
       61 eevnyiecee nsdeehlfcd vcggdhpgdc hkhgplthvk haevdagdpl ranktlpegl
      121 sigrstfkga qygmftlklq pkrvyfgpye gvkvedngra ngytwqvrrt qdafrtikav
      181 v
//
LOCUS       XP_077507348             183 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144116989 [Amblyomma americanum].
ACCESSION   XP_077507348
VERSION     XP_077507348.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651222.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 25% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="uncharacterized protein LOC144116989"
                     /calculated_mol_wt=19801
     CDS             1..183
                     /gene="LOC144116989"
                     /coded_by="XM_077651222.1:1..552"
                     /db_xref="GeneID:144116989"
ORIGIN      
        1 mvgrgraags grvrpsggiq epsghdegrr kgsmlqglnq etigvssipv alvpsdggai
       61 rlnnpeavqa avqstskhfl ritdirqfgr ggilcrspyk dciedllkct sfanhpaggk
      121 mdlqysketh fvsgrrdqtp yphspcvdna arrrglrgrq lkavrihkcl erstfgvvpc
      181 dqr
//
LOCUS       XP_077507349             130 aa            linear   INV 25-NOV-2025
DEFINITION  palmitoyltransferase ZDHHC2-like [Amblyomma americanum].
ACCESSION   XP_077507349
VERSION     XP_077507349.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651223.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..130
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..130
                     /product="palmitoyltransferase ZDHHC2-like"
                     /calculated_mol_wt=14375
     Region          <51..>130
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; cl19890"
                     /db_xref="CDD:418707"
     CDS             1..130
                     /gene="LOC144117035"
                     /coded_by="XM_077651223.1:1..393"
                     /db_xref="GeneID:144117035"
ORIGIN      
        1 matssggses arrrrsnhef faclpvamve gaqrfafstv fhlllllclw sfvqttvtav
       61 ppipgyfgls esdqrlleqc addeargdfl dilaenwgvl trgpsggvrf cercqqvkpn
      121 rahhcsqcrr
//
LOCUS       XP_077507351             413 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144117099 [Amblyomma americanum].
ACCESSION   XP_077507351
VERSION     XP_077507351.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651225.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..413
                     /product="uncharacterized protein LOC144117099"
                     /calculated_mol_wt=42840
     Region          14..97
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     CDS             1..413
                     /gene="LOC144117099"
                     /coded_by="XM_077651225.1:20..1261"
                     /db_xref="GeneID:144117099"
ORIGIN      
        1 mlaefwqlls nealvdvtla ceglslkahk vvlsacspff qalfvenpck hpivilpamr
       61 yidlkaivef myrgevnlpl dqiaallkta etlkvkglae ctnenknggl vtsvdgaada
      121 gpvgtpraes aplskrkgsh gpssrasnva assstavpsg arhgssvqas isqhtqdsig
      181 ddveegddad fdleildvme eftttenvpe fpdvasssqv lgyesqqshh tgftsdnsal
      241 vpaqrslpsd fsitsqvdie pspyslipyd dqaippvvaa fpaaalsgdg asiamavtda
      301 sgvpavagps gyqqstpshg typvpdhpth lhlffsasrf phgdpslcga sgfsftcnag
      361 rcahatiasg pterrslvkl pclwatippv vaaspaaals gdgtsiamav mdv
//
LOCUS       XP_077507352             571 aa            linear   INV 25-NOV-2025
DEFINITION  phosrestin-2-like [Amblyomma americanum].
ACCESSION   XP_077507352
VERSION     XP_077507352.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651226.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..571
                     /product="phosrestin-2-like"
                     /calculated_mol_wt=61113
     Region          156..338
                     /region_name="Arrestin_N"
                     /note="Arrestin (or S-antigen), N-terminal domain;
                     cl22903"
                     /db_xref="CDD:451447"
     Region          362..521
                     /region_name="Arrestin_C"
                     /note="Arrestin (or S-antigen), C-terminal domain;
                     smart01017"
                     /db_xref="CDD:214976"
     CDS             1..571
                     /gene="LOC144117161"
                     /coded_by="XM_077651226.1:1..1716"
                     /db_xref="GeneID:144117161"
ORIGIN      
        1 mkermevqdl mcttetpgpa asqahgarrs sfgvrvtvag aldilaqggv tppghgrdga
       61 adpagpadph syaasaadka gtssedvaia etytppddgd eeelgphttk egaltavtss
      121 artlkeaegn ahpgasgver ihgyrvykkq spnsrltlyl strefvhass qnsvsaidgv
      181 vlvdptylkd rrvfamvllt frygredeev mglkffteai ldfkqvypep sassasgadg
      241 ggpaanrasg cdgssgtlee ltplqrnllk klggdayplt lrispqapps vrlhparpyq
      301 gsplgvsydl rvfigdradd kphkrntvrm alrcvqyada aadaltvrpp sasvrktfff
      361 cpgrldleas ldretyhqgd vvrihvtmsn gssktvqrlk vavlqhvhvc mfthgrfknl
      421 igsadtgpgr tvppgstvsr dfevrlqsce kfpvalaves efheedttay slssttmlln
      481 preknpygvn vsyevkvkai lgcidrplvl rlpfrimlpr pthganrppl pkagtsalkp
      541 edsadsvadc vaedvarltv nsqpsgepda t
//
LOCUS       XP_077507353             106 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144117177 [Amblyomma americanum].
ACCESSION   XP_077507353
VERSION     XP_077507353.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651227.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..106
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..106
                     /product="uncharacterized protein LOC144117177"
                     /calculated_mol_wt=11522
     CDS             1..106
                     /gene="LOC144117177"
                     /coded_by="XM_077651227.1:277..597"
                     /db_xref="GeneID:144117177"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdptyrppfa altsvlccqr pasigpcvlp afgcvippdp
       61 fmlktkscme vvqatklrvi fardleqagk lvddqwysls kfgheg
//
LOCUS       XP_077507354             460 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144117193 [Amblyomma americanum].
ACCESSION   XP_077507354
VERSION     XP_077507354.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651228.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 15% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..460
                     /product="uncharacterized protein LOC144117193"
                     /calculated_mol_wt=51455
     Region          237..339
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     CDS             1..460
                     /gene="LOC144117193"
                     /coded_by="XM_077651228.1:36..1418"
                     /db_xref="GeneID:144117193"
ORIGIN      
        1 mrhpqamcvh tvppdqsaaq thqigcrlql amqndlgqga ascrescrni atcatrpsgq
       61 ppdfelskcf vasgprstig gqaagdhhhl rgkgeevlkl reapaqkytq patavsrspq
      121 pagiklwvgq rqsavvslsp aatvqamvll kmymdgftys hiseaatapi stqytsfnlk
      181 elfitflnil teykvcyrcy krtnfwhwts stcvivstqt etvpefslwr ylgtreladv
      241 eivvhwehap eqhasfkahr milalqnvvf ramffghfar edrltimdmh pdgvegllry
      301 fyseqlqves vhqatctrsv avkymvpela arcvayvery mepddvcpfl dyiltmcedg
      361 vdgsakavlh nnglfvlask tfescllyta nyildnvhna temsvlqavh acgrrqcler
      421 gkddrepagl rsavrpfflk lrflvltvte fvrgpnrgvc
//
LOCUS       XP_077507355             140 aa            linear   INV 25-NOV-2025
DEFINITION  BTB/POZ domain-containing protein 6-B-like [Amblyomma americanum].
ACCESSION   XP_077507355
VERSION     XP_077507355.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651229.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..140
                     /product="BTB/POZ domain-containing protein 6-B-like"
                     /calculated_mol_wt=15690
     Region          <2..75
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     CDS             1..140
                     /gene="LOC144117226"
                     /coded_by="XM_077651229.1:1..423"
                     /db_xref="GeneID:144117226"
ORIGIN      
        1 milalqndvf ramffghfar edrsdlhpdg vegllryiys gqfevesvhq atctrsaavk
       61 ymvpelaarc vaylerymep ddvcpfldyi ltmgedgvdg cgkavlhnng lfvlasktfe
      121 sclhytvnyi ldnvhnapem
//
LOCUS       XP_077507358             233 aa            linear   INV 25-NOV-2025
DEFINITION  BTB/POZ domain-containing protein 6-B-like [Amblyomma americanum].
ACCESSION   XP_077507358
VERSION     XP_077507358.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651232.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 33% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..233
                     /product="BTB/POZ domain-containing protein 6-B-like"
                     /calculated_mol_wt=26108
     Region          <2..79
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     CDS             1..233
                     /gene="LOC144117258"
                     /coded_by="XM_077651232.1:15..716"
                     /db_xref="GeneID:144117258"
ORIGIN      
        1 milalqndvf ramffghfar edrltimdlh pdgvegllry fyseqlqves vhqatctrsa
       61 aveymvpela arcvayvery mepddvcpfl dyiltmgedg adgsakvvlh nnglfvlase
      121 tfesclhyta nyildnvhna pemsvlqavh accsrqcler gkvggepagl hsvvrpfflk
      181 lrflvltvte fvrgpnvwgm lyaeeslail cdiieedslp mpadfctlrt qrv
//
LOCUS       XP_077507359             170 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144117274 [Amblyomma americanum].
ACCESSION   XP_077507359
VERSION     XP_077507359.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651233.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..170
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..170
                     /product="uncharacterized protein LOC144117274"
                     /calculated_mol_wt=18110
     CDS             1..170
                     /gene="LOC144117274"
                     /coded_by="XM_077651233.1:1..513"
                     /db_xref="GeneID:144117274"
ORIGIN      
        1 msvvpaslpt rrspvvvvps rfslcalllp gcalcflrhf aflcvarwka aktqsrnvwa
       61 aaenqlqrve haaegglegl pttphlavig ssmgdikkii vavdstrsle kamglllgaf
      121 fvfnvayppd cpltmellqr ylgqsnptkg rgkgracgia pkllsllnal
//
LOCUS       XP_077507360             140 aa            linear   INV 25-NOV-2025
DEFINITION  BTB/POZ domain-containing protein 6-B-like [Amblyomma americanum].
ACCESSION   XP_077507360
VERSION     XP_077507360.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651234.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..140
                     /product="BTB/POZ domain-containing protein 6-B-like"
                     /calculated_mol_wt=15753
     Region          <2..75
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     CDS             1..140
                     /gene="LOC144117290"
                     /coded_by="XM_077651234.1:1..423"
                     /db_xref="GeneID:144117290"
ORIGIN      
        1 milalqndvf ramffghfar edrpdlhpdg vegllryfys gqfevesvhq atctrsaavk
       61 ymlpelaarc vayverymep ddvcpfldyi ltmgedgvdg cgkavlhnng lfvlasktfe
      121 sclhytvnyi ldnvrnapem
//
LOCUS       XP_077507361             177 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144117306 [Amblyomma americanum].
ACCESSION   XP_077507361
VERSION     XP_077507361.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651235.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..177
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..177
                     /product="uncharacterized protein LOC144117306"
                     /calculated_mol_wt=20664
     Region          91..164
                     /region_name="BTB_POZ_ZBTB_KLHL-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in zinc finger and
                     BTB domain-containing (ZBTB) proteins, Kelch-like (KLHL)
                     proteins, and similar proteins; cd18186"
                     /db_xref="CDD:349497"
     CDS             1..177
                     /gene="LOC144117306"
                     /coded_by="XM_077651235.1:1..534"
                     /db_xref="GeneID:144117306"
ORIGIN      
        1 mvllkmymdg fpyshiseaa tapistqyts fnlnelfitf lnilteykvc yrcykrtnfw
       61 hwtsstrviv stqtetvpef slwrylgtre ladveivvhw ehapehhasf kahrmilalq
      121 ndvframffg hfaredrlti mdlhpdgveg llryfyseql qvesvhqatc trsaave
//
LOCUS       XP_077507362             396 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144117354 [Amblyomma americanum].
ACCESSION   XP_077507362
VERSION     XP_077507362.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651236.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 36% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..396
                     /product="uncharacterized protein LOC144117354"
                     /calculated_mol_wt=42141
     Region          14..97
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     CDS             1..396
                     /gene="LOC144117354"
                     /coded_by="XM_077651236.1:9..1199"
                     /db_xref="GeneID:144117354"
ORIGIN      
        1 mlaefwqlls nealvdvtla ceglslkahk vvlsacspff qalfvenpce hpivilpamr
       61 yidlkaivef myrgevnvpl eqiaallkta etlkvkglae ctnenknggh vtsvdgaada
      121 gpvgtpraes aplskrkgsr gpssrasnva vssstavpsg arhgssvqas isqhtqdsig
      181 ddmeegddad fdleildvme eftttenvpe fpdvasssqv lgyesqqshh tgftsdnsal
      241 vpaqrslpsd fsitsqvdie pspyslipyd dqaippvvaa fpaaalsgdg asiamavtda
      301 sgvpavagps gyqqstpshg typvpdhpth lhlffsayla rgqdikdial ayrvgietar
      361 vaihfccrii warlkdqfmk vpsegdwvei aegfkk
//
LOCUS       XP_077507363             156 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144117387 [Amblyomma americanum].
ACCESSION   XP_077507363
VERSION     XP_077507363.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651237.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 21% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..156
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..156
                     /product="uncharacterized protein LOC144117387"
                     /calculated_mol_wt=17717
     Region          28..>142
                     /region_name="TBP_TLF"
                     /note="TATA box binding protein (TBP): Present in archaea
                     and eukaryotes, TBPs are transcription factors that
                     recognize promoters and initiate transcription. TBP has
                     been shown to be an essential component of three different
                     transcription initiation complexes:...; cl08263"
                     /db_xref="CDD:447593"
     Site            order(42,49..51,53,58,64,66)
                     /site_type="other"
                     /note="TFIIA interaction surface [polypeptide binding]"
                     /db_xref="CDD:239601"
     CDS             1..156
                     /gene="LOC144117387"
                     /coded_by="XM_077651237.1:1..471"
                     /db_xref="GeneID:144117387"
ORIGIN      
        1 magectvmrq hedavgelql lplahrrniv atvnlccqld lrqialrarn veynprrfaa
       61 vimrsrehrt tallfqsgki vytgvrselq ariaarkfar itqkfgynar fadfkvqniv
      121 ascdvgfcik leslalnhsq fsrrkvrpdh sarygq
//
LOCUS       XP_077507364             567 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144117403 [Amblyomma americanum].
ACCESSION   XP_077507364
VERSION     XP_077507364.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651238.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 45% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..567
                     /product="uncharacterized protein LOC144117403"
                     /calculated_mol_wt=60883
     Region          112..304
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     Region          <323..>479
                     /region_name="PRK07003"
                     /note="DNA polymerase III subunit gamma/tau"
                     /db_xref="CDD:235906"
     CDS             1..567
                     /gene="LOC144117403"
                     /coded_by="XM_077651238.1:39..1742"
                     /db_xref="GeneID:144117403"
ORIGIN      
        1 mvqsdhtyag psteasassl epgtagvaat lsasadlspg apfsssplsy egsftspine
       61 rrplqklrak lrqlrnrnkr lqglllqrrr mkttaelvds lrehltpava sfveaqlkmh
      121 nvsrfgrrwc sqnktfalgl yfhspkgyry crkllklpsv rslqlwlarv rlrvgfypev
      181 fdliekraas fprqdracti ifdemhvske lsynpvpdrf egleeysaaq gpnlankalv
      241 fmakgictpw kqplgyffad kaapatvlyd llfkchkmlv gaglqpvavv cdqgnqnvsl
      301 fsklvtpekp laqyalqatk ifaagsvlaa gnevpllqma apvvyvtvfr lppsvsddal
      361 aaalspygkp aagveplsgr pespaepvvp saaesddtsp tvaitgepag aipevvsdee
      421 kaekssspee dseastsssv psgvtdteid rfhsqptagq rnqddlepes sgtpseasaf
      481 vfpkgdpswt npisqvadll hpgtqqscsi pdtpstasla alqrqrsapr lsgtrmvskg
      541 hgideakrql sssdggaasa yptvlkk
//
LOCUS       XP_077507365             152 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144117451 [Amblyomma americanum].
ACCESSION   XP_077507365
VERSION     XP_077507365.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651239.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..152
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..152
                     /product="uncharacterized protein LOC144117451"
                     /calculated_mol_wt=17184
     Region          28..>142
                     /region_name="TBP_TLF"
                     /note="TATA box binding protein (TBP): Present in archaea
                     and eukaryotes, TBPs are transcription factors that
                     recognize promoters and initiate transcription. TBP has
                     been shown to be an essential component of three different
                     transcription initiation complexes:...; cl08263"
                     /db_xref="CDD:447593"
     Site            order(42,49..51,53,58,64,66)
                     /site_type="other"
                     /note="TFIIA interaction surface [polypeptide binding]"
                     /db_xref="CDD:239601"
     CDS             1..152
                     /gene="LOC144117451"
                     /coded_by="XM_077651239.1:1..459"
                     /db_xref="GeneID:144117451"
ORIGIN      
        1 magectvmrq hedavgelql lplahrrniv atvnlccqld lrqialrarn veynprrfaa
       61 vimrsrehrt tallfqsgki vytgvrselq ariaarkfar itqkfgynar fadfkvqniv
      121 ascdvgfcik leslalnhsq fsrvisdgif mr
//
LOCUS       XP_077507366             509 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144117483 [Amblyomma americanum].
ACCESSION   XP_077507366
VERSION     XP_077507366.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651240.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 50% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..509
                     /product="uncharacterized protein LOC144117483"
                     /calculated_mol_wt=54665
     Region          <278..>421
                     /region_name="PRK07003"
                     /note="DNA polymerase III subunit gamma/tau"
                     /db_xref="CDD:235906"
     CDS             1..509
                     /gene="LOC144117483"
                     /coded_by="XM_077651240.1:1..1530"
                     /db_xref="GeneID:144117483"
ORIGIN      
        1 mcfffmmqvs tyfvkeafny dkdnvtlkam pgltlshldp nnfekmrvtl afqlfgdrvl
       61 rglhhykdrl essygkgaid atekffrmin eliktmtsry ksealwpnsr gaaalsafle
      121 yitkweectv ngvgfisktt avgfrvtlsg vlrlleyltk evgfkylmta klsqdpvenl
      181 fgvvrqssgc ndhptpeqfl itvnclsfys larpvdgasv eplvltalld tgntdlsnst
      241 tledtidsyi srgdldsled aasqkgngvp tydhettacq vacrrcggth atsdcvaprs
      301 yaaalatlpa qpaagveplp grpesptepv vpsaaesddt sptvaitgep agaipevvsd
      361 eekaeksssp eedseastss svpsgvtdte idrfhsqpta gqrnqddlep essdtpsevs
      421 afvlpkgdps wtnpisqvad llhpgtqqsc sipdtpstas laalqrqrsa prlsgtrmvs
      481 kghgideakr qlsssdggaa sayptalkk
//
LOCUS       XP_077507367              69 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin-like domain-containing CTD phosphatase 1 [Amblyomma
            americanum].
ACCESSION   XP_077507367
VERSION     XP_077507367.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651241.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..69
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..69
                     /product="ubiquitin-like domain-containing CTD phosphatase
                     1"
                     /calculated_mol_wt=8195
     Region          <9..67
                     /region_name="HAD_like"
                     /note="Haloacid Dehalogenase-like Hydrolases; cl21460"
                     /db_xref="CDD:473868"
     CDS             1..69
                     /gene="LOC144117499"
                     /coded_by="XM_077651241.1:42..251"
                     /db_xref="GeneID:144117499"
ORIGIN      
        1 middirrnvd nirrnllmnp qsglrvrpfr nahphrntdr elvrlarylk diacsvsdft
       61 elnhrnwkt
//
LOCUS       XP_077507368              69 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin-like domain-containing CTD phosphatase 1 [Amblyomma
            americanum].
ACCESSION   XP_077507368
VERSION     XP_077507368.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651242.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..69
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..69
                     /product="ubiquitin-like domain-containing CTD phosphatase
                     1"
                     /calculated_mol_wt=8195
     Region          <9..67
                     /region_name="HAD_like"
                     /note="Haloacid Dehalogenase-like Hydrolases; cl21460"
                     /db_xref="CDD:473868"
     CDS             1..69
                     /gene="LOC144117531"
                     /coded_by="XM_077651242.1:42..251"
                     /db_xref="GeneID:144117531"
ORIGIN      
        1 middirrnvd nirrnllmnp qsglrvrpfr nahphrntdr elvrlarylk diacsvsdft
       61 elnhrnwkt
//
LOCUS       XP_077507369             121 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144117547 [Amblyomma americanum].
ACCESSION   XP_077507369
VERSION     XP_077507369.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651243.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..121
                     /product="uncharacterized protein LOC144117547"
                     /calculated_mol_wt=11918
     CDS             1..121
                     /gene="LOC144117547"
                     /coded_by="XM_077651243.1:500..865"
                     /db_xref="GeneID:144117547"
ORIGIN      
        1 mdcsqslset sasvrragep aavsgepvsk gyvqsrssqs lllfqedadg asptgstlnq
       61 acshasgfim vqsdhtyagp steasassle pgtagvaatl sasadlspga pfsssplsye
      121 g
//
LOCUS       XP_077507370              69 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin-like domain-containing CTD phosphatase 1 [Amblyomma
            americanum].
ACCESSION   XP_077507370
VERSION     XP_077507370.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651244.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..69
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..69
                     /product="ubiquitin-like domain-containing CTD phosphatase
                     1"
                     /calculated_mol_wt=8195
     Region          <9..67
                     /region_name="HAD_like"
                     /note="Haloacid Dehalogenase-like Hydrolases; cl21460"
                     /db_xref="CDD:473868"
     CDS             1..69
                     /gene="LOC144117579"
                     /coded_by="XM_077651244.1:36..245"
                     /db_xref="GeneID:144117579"
ORIGIN      
        1 middirrnvd nirrnllmnp qsglrvrpfr nahphrntdr elvrlarylk diacsvsdft
       61 elnhrnwkt
//
LOCUS       XP_077507371             827 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144117595 [Amblyomma americanum].
ACCESSION   XP_077507371
VERSION     XP_077507371.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651245.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..827
                     /product="uncharacterized protein LOC144117595"
                     /calculated_mol_wt=91733
     Region          181..373
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..827
                     /gene="LOC144117595"
                     /coded_by="XM_077651245.1:317..2800"
                     /db_xref="GeneID:144117595"
ORIGIN      
        1 mdcsqslset sasvrragep aavsgepvsk gyvqsrssqs lllfqedadg asptgstlnq
       61 acshasgfim vqsdhtyagp steasassve pgtagvaatl sasadlspga pfsssplsye
      121 gsftspiner rplqklrakl rqlrnrnkrl qglllqrrrm kttaelvdsl rehltpavas
      181 fvepqlkmhn vsrfgrrwcs qnktfalgly fhspkgyryc rkflklpsvr slqlwlarvp
      241 lrvgfypevf dliekraasf prqdraftii fdemhvskel synpvpdrfe gleeysaaqg
      301 pnlankalvf makgictpwk qplgyffadk aapatvlydl lfkchkmlvg aglqpvavvc
      361 dqgnqnvslf sklvtpekpy lsvngeplfv ifdpphllkc lrnmlfkydf rvgsdtikss
      421 yirqayekdr emqirsipkl sawhfkltfa skmsakiaaq vfsnycaaal ctlvtfqqlp
      481 seaihtarfi eridrlfdsl ntsqkrgksp yasaicagsv heefleecia vfenievlgc
      541 arqplcirgf cqtmravlll wnhlasrygl tylltrhlnq dalentfaiv rsksgsnsns
      601 scrqfqaafr hllisnlfkl sessncaedm ssllatlpvc lsksapslct aatslpvave
      661 vlphdyqspi lennivyfag wlaykfmndh rcvtgphtck vrlknaafad qsqvllylsv
      721 knpgegdfgs lsvptpsfva finaceqvfv asssnivgke qvghdlcmll hakveksltv
      781 cgddvydglf alfarvrlhw larrknlelh sqttkrkatk qalrlsk
//
LOCUS       XP_077507372             786 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077507372
VERSION     XP_077507372.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651246.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..786
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=86257
     Region          109..236
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          328..734
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..786
                     /gene="LOC144117627"
                     /coded_by="XM_077651246.1:1..2361"
                     /db_xref="GeneID:144117627"
ORIGIN      
        1 mapwvivafs lvlveitssq qtlsftehas qsqagapgqp svsslgaada gsaaaavase
       61 raldaggdsd depapdveaf vhdtidaayk kalpmvtrfg fdpsvsaecs qsllkmalaf
      121 rrlepwairm ldasgkvasg llvgtigdlg nfdeclattv enmygerdft grfctvymnf
      181 knntfvakmv arfqekgylr dyknplnwiq nkmahgvrqs acipstcsak dlsyivntlg
      241 kkigmkalir gckaegetki lgwqagilyt latvailvaa ataadvalrt vlkrrmstkp
      301 kgilydvvyg fsvvsntekl lktdpsdpad sklkfihglr fysstwvmlg hafliadpta
      361 tgslmnvvdl vedflttlia nafpsvetfl fisgflvsyn vqktlrnktk lifwvpliil
      421 rryirltvpa mfvvgvwlll plmadgpvln dykatfldtc qrrwwtvmlh tnnfspflem
      481 clghlwyini dfqlyvallf iplvmirrpy lgalfaalat vvssaytgvv sylnnyqpav
      541 ifthtdvsyt igtanqiyfk pfahvgsfci gvllgyyiyv apkmklskpv qvlcwcaata
      601 cnlaivlaph kwytdhsysi kqpecllyas lhrvawtlgv awlvfacatg rggivmslls
      661 wpalvplsrl sygaflchvv illvqmmtnr eriaytymvk tmnylavvma sylcayllyl
      721 fceapvatle rtllggigsp rrggpasrlt vivpkaaeqh lppgsslrel patpaaaees
      781 sqrshl
//
LOCUS       XP_077507373             429 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144117643 [Amblyomma americanum].
ACCESSION   XP_077507373
VERSION     XP_077507373.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651247.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..429
                     /product="uncharacterized protein LOC144117643"
                     /calculated_mol_wt=44953
     Region          30..113
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     CDS             1..429
                     /gene="LOC144117643"
                     /coded_by="XM_077651247.1:82..1371"
                     /db_xref="GeneID:144117643"
ORIGIN      
        1 merpqfcvkw khhhsnmlae fwqllsneal vdvtlacegl slkahkvvls acspffqalf
       61 venpcehpiv ilpamryidl kaivefmyrg evnvpleqia allktaetlk vkglaectne
      121 nkngglvtsv dgaadagpvc tpraesapls krkgsrgpss rasnvaasss tavpsgarhg
      181 ssvqasisqh tqdsigddve egddadfdle ildvmeeftt tenvpefpdv asssqvlgye
      241 sqqshhtgft sdnsalvpaq rslpsdfsit sqvdiepspy slipyddqai ppvvaafpaa
      301 alsgdgasia mavtdasgvp avagpsgyqq stpshgtypv pdhpthlhlf fsasrfphgd
      361 pslcgasgfs ftcnagrcah atiasgpter rslvklpclw atippvvaas paaalsgdgt
      421 siamavmdv
//
LOCUS       XP_077507374             419 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144117706 [Amblyomma americanum].
ACCESSION   XP_077507374
VERSION     XP_077507374.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651248.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 40% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..419
                     /product="uncharacterized protein LOC144117706"
                     /calculated_mol_wt=44680
     Region          <13..>76
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     CDS             1..419
                     /gene="LOC144117706"
                     /coded_by="XM_077651248.1:202..1461"
                     /db_xref="GeneID:144117706"
ORIGIN      
        1 mggalakflt kdhplqslsl incsfdsrga thiasalsan ssleclnvqn slpaeelvsk
       61 lcsglrankt lrslvlpeit stssllqkgs ptlellrsel rvqfslfvrl pslvspalgq
      121 alrcatkvmi ehsalagdim ssvyaslaln hsllslsiey vgdgpagdgt tegghigalc
      181 stlktcarlq tfkldvrray desgdmvlak vfdalnhttc lrklsvvtsr ltlntaqsls
      241 alvaqckrcl ielhivsvgp ipdavlgvlh emvirnvfls risvggsawd davrasvaid
      301 dakmhnqrll nkaarfvmrl dgmpealpdh ncaaafdemc gaaslryhlv alsgkseaqv
      361 slnvkrarry llenfmvlag vvhaavvcna gvgttqldal ngdcwcaiaq ylklsdvir
//
LOCUS       XP_077507375             193 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144117722 [Amblyomma americanum].
ACCESSION   XP_077507375
VERSION     XP_077507375.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651249.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..193
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..193
                     /product="uncharacterized protein LOC144117722"
                     /calculated_mol_wt=21998
     CDS             1..193
                     /gene="LOC144117722"
                     /coded_by="XM_077651249.1:230..811"
                     /db_xref="GeneID:144117722"
ORIGIN      
        1 meleglkqgl aflesanikv avlvtdrhtq ikcflrnnka aithefdvwh makgitkklh
       61 taakqnscke lapwiksvcn hlywaaasse gkaqlivpkw lsllnhirdi hthdqalfpm
      121 clhgdiepkq wlreeseafq klediakska llqdmprlst rhqtygleaf hsllvhftpk
      181 tyhysysgmr asn
//
LOCUS       XP_077507376             337 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144117738 [Amblyomma americanum].
ACCESSION   XP_077507376
VERSION     XP_077507376.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651250.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 37% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..337
                     /product="uncharacterized protein LOC144117738"
                     /calculated_mol_wt=37244
     Region          <63..252
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..337
                     /gene="LOC144117738"
                     /coded_by="XM_077651250.1:1..1014"
                     /db_xref="GeneID:144117738"
ORIGIN      
        1 mtdeyavqag vrgevygetv akkkvatlkv evkrlqrlhq svgklsergk lvfhqnamka
       61 naksaravry kkewlcdcll lkidstaayt flhedgflpl pnprtlysym knlkadfgfd
      121 aslftllket lqevpereqr gvlmfdemsv qkslhgresd mkvlgkvnlg ehtrpgdqek
      181 dadhvlvflf rpflggwskt vgtlcassas pgsviaklll eciahllnsg vvvdavtcdn
      241 stsnrsalrs lgddsngqip pearnrtnsm dsdrkvsarq pwllrkegvs sleirarlva
      301 vygeqaaslr tvfyrvegwk agrkssamgp aldgrql
//
LOCUS       XP_077507377             166 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier organic anion transporter family member 4A1-like
            [Amblyomma americanum].
ACCESSION   XP_077507377
VERSION     XP_077507377.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651251.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..166
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..166
                     /product="solute carrier organic anion transporter family
                     member 4A1-like"
                     /calculated_mol_wt=17719
     Region          32..>154
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     CDS             1..166
                     /gene="LOC144117754"
                     /coded_by="XM_077651251.1:436..936"
                     /db_xref="GeneID:144117754"
ORIGIN      
        1 mytvharsrs fwrvlarsrp vkgavvvdgv qvfrsmlnva scimvtpvsy hggmggrpvi
       61 lgigalvtsv gamifasphf iapqyrvlen gtasvcperg pgkcslsgra tnanafkyff
      121 mlyhamhgvg sspfftvgva yldqnvptgs asvymvnykl awscsd
//
LOCUS       XP_077507378             143 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144117770 [Amblyomma americanum].
ACCESSION   XP_077507378
VERSION     XP_077507378.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651252.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 21% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..143
                     /product="uncharacterized protein LOC144117770"
                     /calculated_mol_wt=16082
     CDS             1..143
                     /gene="LOC144117770"
                     /coded_by="XM_077651252.1:674..1105"
                     /db_xref="GeneID:144117770"
ORIGIN      
        1 mrkkpchrql pfdrnqndre aavaglrqgt gnaqatarps lqarrvavmp sgkkdtpeka
       61 flrhqvkrya aihvftkkki krlqqaqrrl qrkvarlssv iddlsknkil iegniaalqs
      121 lagskenllm rhivektdii kma
//
LOCUS       XP_077507379             144 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144117786 [Amblyomma americanum].
ACCESSION   XP_077507379
VERSION     XP_077507379.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651253.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..144
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..144
                     /product="uncharacterized protein LOC144117786"
                     /calculated_mol_wt=15435
     Region          16..>133
                     /region_name="DEAD-like_helicase_N"
                     /note="N-terminal helicase domain of the DEAD-box helicase
                     superfamily; cl28899"
                     /db_xref="CDD:475120"
     CDS             1..144
                     /gene="LOC144117786"
                     /coded_by="XM_077651253.1:1..435"
                     /db_xref="GeneID:144117786"
ORIGIN      
        1 mpvwiaevka afgsgdsmag akrgsgtasp hvhqalrtlv ghnfksrank wvhvlpplip
       61 sptplwrvlc vhkcaldsiq naavvcgarr wtsssscrhr lslgvhvvaa ttgrlkdfve
      121 ksnifgklrf smvgatrlps dmgr
//
LOCUS       XP_077507380             942 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077507380
VERSION     XP_077507380.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651254.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..942
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=104576
     Region          <44..>161
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     Region          126..912
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          544..913
                     /region_name="Piwi-like"
                     /note="PIWI domain. Domain found in proteins involved in
                     RNA silencing. RNA silencing refers to a group of related
                     gene-silencing mechanisms mediated by short RNA molecules,
                     including siRNAs, miRNAs, and heterochromatin-related
                     guide RNAs. The central component...; cl00628"
                     /db_xref="CDD:412485"
     Site            order(676,680,692..695,698,707,710,714,718)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     CDS             1..942
                     /gene="LOC144117880"
                     /coded_by="XM_077651254.1:57..2885"
                     /db_xref="GeneID:144117880"
ORIGIN      
        1 mgtgkrkdkk kndsaagppa vpsasggnqa papapspagp srvsfaqalq qgaapspqpr
       61 avsrgptpaa etraqptaqp taqpsasrps ygaaaaaagd anvaassprp asaaaavsrs
      121 qpaaaalpps ssctppvavt eqtpeagdqa tpgddvrike lertlpshfp rrpahgqlgr
      181 tiqllanhfs ieiptgsvyh ydvdisseta ketkvpeqkn yrclstkinr iviellvkky
      241 rqdlancipa fdgrknlytr rqlnfrertf tvdleedqgs qtfiikiqya atvnldalhg
      301 vfqkrvqtvp qevlqaidiv lrhspsinla pvgrsffrpp gpnehndlgg grevwfgyyt
      361 svrpaqwkpm lnvdmsataf yeslplvdfm crffsdsrrv ltpadlrslr dnqyarlnre
      421 lkglrvkvth lpyprkykvv kitreaakdi yfesegsqis vadyfqsryr plsypnfpcv
      481 qsgspthpvy iplevcelae gqhcrkklee sqtaemikrt akppakrfle irqsvrdmvs
      541 tsdkylrefg vkinpeptqv vgrildppsl vfennsmckp rdgtwdlrgq rfykamsmtq
      601 wivlnvsrfa qkecldnfik mlirigqelg mriaqplavi tfdtnrkpmr tvlteqrkqr
      661 pqlemvvavi tkatnyaeik qvaetelslr tqcildnnvv qkcnaaliqn lcqkinakmg
      721 ginnsllmqe kpkllhrpvi vigadvshps pgdkirpsia acvgsldsvp skfhatirvq
      781 iedskakarv eiikdlkdmi kelllvfyrt tghkplqivf yrdgvsegqf levrnhevsa
      841 irlacaemcp tetyeppltf ivvqkrhhtr fmpandrdgv gkfrnvppgt tvdsvvthpl
      901 dfdfflcshf giqvgdlfsa lvaivsgkkw savfssinlr rk
//
LOCUS       XP_077507381             358 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144117896 [Amblyomma americanum].
ACCESSION   XP_077507381
VERSION     XP_077507381.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651255.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 45% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..358
                     /product="uncharacterized protein LOC144117896"
                     /calculated_mol_wt=39642
     Region          163..300
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..358
                     /gene="LOC144117896"
                     /coded_by="XM_077651255.1:1..1077"
                     /db_xref="GeneID:144117896"
ORIGIN      
        1 mrdkalgawp sshdaraarr aaqpfrcdst rlpdrdnsts edpsnkslqm alsviktttq
       61 elaiqrkqev rkyaldsilc hasvrpspss agdagpeapa ppvnrrfcgp tmsmevtveg
      121 tdldpehyds swvkvlqrkv eakhqrklle gskenakspg endaevfrnr kgyfsinvqa
      181 itgpklqfld lvaswpgsah dsrifdnsra rvqyeqgdip gillgdmgya crpylmtplr
      241 ddvlpgspqy kynksqirtr csiertfgvw krrfpcldmt lqiktatvpi vitacaalhn
      301 lgrllkdpvp pallydatrd ctapvpvsra aqppatvplv dsasgfrtrd riiaqyfh
//
LOCUS       XP_077507382             847 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077507382
VERSION     XP_077507382.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651256.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..847
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=94162
     Region          <37..>146
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     Region          119..831
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     CDS             1..847
                     /gene="LOC144117958"
                     /coded_by="XM_077651256.1:1..2544"
                     /db_xref="GeneID:144117958"
ORIGIN      
        1 mnkitnfqgc yqpvpsasgg nqapapapsp agpsrvsfaq alqqgaapsp qpravsrgpt
       61 paaetraqpt aqptaqpsas rpsygaaaaa agdanvaass prpasaaaav srsqpaaaal
      121 ppsssctppv avteqtpeag dqatpgddlr ikelertlps hfprrpahgq lgrtiqllan
      181 hfsieiptgs vyhydvdiss etaketkvpe qknyrclstk inriviellv kkyrqdlanc
      241 ipafdgrknl ytrrqlnfre rtftvdleed qgsqtfiiki qyaatvnlda lhgvfqkrvq
      301 tvpqevlqai divlrhspsi nlapvgrsff rppgpnehnd lgggrevwfg yytsvrpaqw
      361 kpmlnvdmsa tafyeslplv dfmcrffsds rrvltpadlr slrdnqyarl nrelkglrvk
      421 vthlpyprky kvvkitreaa kdiyfesegs qisvadyfqs ryrplsypnf pcvqsgspth
      481 pvyiplevce laegqhcrkk leesqtaemi krtakppakr fleirqsvrd mvstsdkylr
      541 efgvkinpep tqvvgrildp pslvfennsm ckprdgtwdl rgqrfykams mtqwivlnvs
      601 rfaqkecldn fikmlirigq elgmriaqpl avitfdtnrk pmrtvlteqr kqrpqlemvv
      661 avitkatnya eikqvaetel slrtqcildn nvvqkcnaal iqnlcqkina kmgginnsll
      721 mqekpkllhr pvivigadvs hpspgdkirp siaacvgsld svpskfhati rvqiedskak
      781 arveiikdlk dmikelllvf yrttghkplq ivfyrdgvse gqflevrnhe vsncaycntv
      841 stklssc
//
LOCUS       XP_077507383             212 aa            linear   INV 25-NOV-2025
DEFINITION  myb/SANT-like DNA-binding domain-containing protein 3 [Amblyomma
            americanum].
ACCESSION   XP_077507383
VERSION     XP_077507383.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651257.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..212
                     /product="myb/SANT-like DNA-binding domain-containing
                     protein 3"
                     /calculated_mol_wt=23472
     Region          8..85
                     /region_name="Myb_DNA-bind_5"
                     /note="Myb/SANT-like DNA-binding domain; pfam13873"
                     /db_xref="CDD:433544"
     CDS             1..212
                     /gene="LOC144117974"
                     /coded_by="XM_077651257.1:1..639"
                     /db_xref="GeneID:144117974"
ORIGIN      
        1 mekmekgkin fsrveralll dlvdkhkavl enkrtdavsv arkrkeweli etqfnsshnv
       61 sprtwlqlkk cwenwknkwr kakaddnrei fktgggsaap sqltedlqrv gsvashmgvr
      121 lanpfdsdrh qadpggesqp tpsvaallsr tqdgsipgas gdaedplwsw hyeegspqqd
      181 eqmeeeqvam aasltsatta taapstgraf wl
//
LOCUS       XP_077507384             530 aa            linear   INV 25-NOV-2025
DEFINITION  serine/threonine-protein kinase PLK1-like [Amblyomma americanum].
ACCESSION   XP_077507384
VERSION     XP_077507384.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651258.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..530
                     /product="serine/threonine-protein kinase PLK1-like"
                     /calculated_mol_wt=59221
     Region          25..279
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(33..36,39,41,54,56,88,104..107,150,154..155,157,
                     167..168)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     Region          345..433
                     /region_name="POLO_box_1"
                     /note="First polo-box domain (PBD) of polo-like kinases
                     Plk1, Plk2, Plk3, and Plk5; cd13118"
                     /db_xref="CDD:240561"
     Site            order(351..354,423,425..429)
                     /site_type="other"
                     /note="phosphopeptide binding site [polypeptide binding]"
                     /db_xref="CDD:240561"
     Region          450..526
                     /region_name="POLO_box_2"
                     /note="Second polo-box domain (PBD) of polo-like kinases
                     Plk1, Plk2, Plk3, and Plk5; cd13117"
                     /db_xref="CDD:240560"
     Site            order(455,474,476,478)
                     /site_type="other"
                     /note="phosphopeptide binding site [polypeptide binding]"
                     /db_xref="CDD:240560"
     CDS             1..530
                     /gene="LOC144118021"
                     /coded_by="XM_077651258.1:1..1593"
                     /db_xref="GeneID:144118021"
ORIGIN      
        1 mdrgtsaaat fnicpeivvd ptsnrqyarg aflgqggcas cfefvdiktq aayagkvisk
       61 tlfadgvgke tvdreihihk slnhkhvvsl eshfedeehv yiilelcngg slydllqtrk
      121 tltedeaanf lrqlllacry lrqqkvlhrd lkpgnlllnd nmelkvgdfg hstrvdeese
      181 miasmqgtig ytapevltgk ecsyeadiws igcilytmlv grppfeadtv qetsrrikqc
      241 dysvpctvss saraliekil dpkpkrrpsi eailrddfit ssepedasss tateeewvpg
      301 pvlngtqqem hvgelqwllq dfhsvspper dielldeaad pavgpdfwis rwadytddng
      361 lgyqlcdssv gvlfcdntcv mlqndgvnvi yvdedglenr hllkdypssl kkkiillkst
      421 ccfleqrlvn tgrcvrrpae gdslvqppcl gtwlrsqdsi sfyltngtlq infkdhtkvi
      481 lcpsmaavsy insrgfhtyr lqtlkqgcpq dlltrlkfar svlqhlgsef
//
LOCUS       XP_077507385             861 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118037 [Amblyomma americanum].
ACCESSION   XP_077507385
VERSION     XP_077507385.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651259.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 32% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..861
                     /product="uncharacterized protein LOC144118037"
                     /calculated_mol_wt=93433
     Region          <367..440
                     /region_name="Prominin"
                     /note="pfam05478"
                     /db_xref="CDD:461660"
     Region          <592..861
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     CDS             1..861
                     /gene="LOC144118037"
                     /coded_by="XM_077651259.1:1..2586"
                     /db_xref="GeneID:144118037"
ORIGIN      
        1 mavrstghng grrlalallv lwqactsaga seasqnqtds nasatiatys veddgstcif
       61 mssigtgill gitdspedas vleeaaqypn agavkelisy hqrgvnvfft vlwgviiffa
      121 ifmiaaiiqf iyimctgqfq hhvresnmgv yifcviaviv lllvmivllv clmgcrsalv
      181 dstddkvpav ysstfdnlrk fvrvtavess rvddrsprvk hdalerlwps nnrslllski
      241 tpskvmrncv ndsvpslals rklnhrkyve rnfyhdpaqr lrmeyelhns plsshrdsni
      301 nsaddsfpfd eilclpngst slleqptrtc rgdvrkslgk ltalggtgvq lymddvektm
      361 lrkapfyfsr fvervvdavh ssastgdgrc stlrsilssf mdvvcytyvl elvsywaslm
      421 latfaailvi pfcfvlskyf frtkrkkikk rrvrevaess slsrrrvref eesssltelp
      481 pqadtssgys vslsfttqee ptdevtrrvv yqsrsshiyd pfahiyapai rrqtsftsll
      541 triplqhirr ihsaasyapq piafsrsvic hhhnpptlpv maapvhspsc ilsvppapvv
      601 lqsprstrvv hqmhshtava phlhrrsrta vvstnrnval spsvvcvssp ptiltqpptv
      661 laapsrvltk sttvltqqpt vvtqqptllt ppsvlapppp ptvplvvspp ppappvttsp
      721 vgsgpaenqq aatahmtinf ygspaggaft mpmtgyplgy pttgyvaaag qpyyyaaapv
      781 gyaatapaat ptvaaaqpas faapacqyac avpqpqsvst hsvstrsvcs rpsspvvarv
      841 paattmtaqp artqlyavpp p
//
LOCUS       XP_077507386             430 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118069 [Amblyomma americanum].
ACCESSION   XP_077507386
VERSION     XP_077507386.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651260.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..430
                     /product="uncharacterized protein LOC144118069"
                     /calculated_mol_wt=47754
     Region          256..415
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..430
                     /gene="LOC144118069"
                     /coded_by="XM_077651260.1:1..1293"
                     /db_xref="GeneID:144118069"
ORIGIN      
        1 mldmgyekks msdykdadtp leeyeaclap ddmveepksd evatavvvpd maeeaildqv
       61 liptmataet aeemtedelt pavaapemad dgipdeaapa meqspevrta stqwedqnne
      121 hsycqrglgt lsdkqqlsaa tlgeddcvfy tglgkthfqn lvtavkvesr klftvlpyed
      181 qllltlmrlr lgllyghlar ifqisvgsvc nifrhvlsvl ctimekvvtw lprsiirnsm
      241 pksfidngyd tttcivdcse inlqrpkklt praqtyssyk ahntvkflia iapdgyimyv
      301 spayggrasd kyitkdckle dhlgpgdevm adrgftltts miiqgvklnv paftkgksql
      361 seeevtrtrr iasvrihver ainriktyri fnqalpiksk ktisdmilvc aglcnlkgpl
      421 iatrreptte
//
LOCUS       XP_077507387             202 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118085 [Amblyomma americanum].
ACCESSION   XP_077507387
VERSION     XP_077507387.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651261.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 11% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..202
                     /product="uncharacterized protein LOC144118085"
                     /calculated_mol_wt=22404
     Region          17..181
                     /region_name="TesA"
                     /note="Lysophospholipase L1 or related esterase. Includes
                     spore coat protein LipC/YcsK [Cell cycle control, cell
                     division, chromosome partitioning, Lipid transport and
                     metabolism]; COG2755"
                     /db_xref="CDD:442045"
     CDS             1..202
                     /gene="LOC144118085"
                     /coded_by="XM_077651261.1:1..609"
                     /db_xref="GeneID:144118085"
ORIGIN      
        1 myqrgnlgse rlerrmvlvv gdsnvarveg vvlatvkadr rvqveaqsgk cmvdamakaq
       61 evvggnmgge hlvvihagln dvlkgrsqnl ekqlkvgmhr lredsermhv tictipevqr
      121 qaseterrvv eanrvirlms rrlrygatev nrevyevrph plaqdgihyg gatgkrlgsr
      181 igrqataffl gsralrdqcf ac
//
LOCUS       XP_077507388            1248 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077507388
VERSION     XP_077507388.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651262.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 34% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1248
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=138198
     Region          35..79
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(37,48,50,53,56,67,70)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          <180..>391
                     /region_name="COG4913"
                     /note="Uncharacterized conserved protein, contains a
                     C-terminal ATPase domain [Function unknown]"
                     /db_xref="CDD:443941"
     Region          <417..>494
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     Region          559..1244
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     CDS             1..1248
                     /gene="LOC144118116"
                     /coded_by="XM_077651262.1:1..3747"
                     /db_xref="GeneID:144118116"
ORIGIN      
        1 mapasaqtvf gfgtgldwrp tcfvspfpss rvcspcclvp ssaamlacrh llcqscydga
       61 sakrshcpld kepfqeddva wttigrdgil grkvrcwnae hgcdnegvas amlehftnac
      121 qfhavsctrc seevlhrdia ehlecdcvps rpqeqplddn fgnafmevke algkilegna
      181 avrrklysfe drlrpeagst fatqststad taivalensi pvlraqaeat lleyravqaa
      241 lnklseenav lhtkldllee hldmknamat lstivsdals tavlktaavc raetaaaldk
      301 rwgqapteir qamagsvrav kgvisrecqr nvlglkasiv eafrgdrrla daratsseta
      361 eklteivdde lkamellvva slsstndfqn ksvpyewtve dwtefctkap lsaqyftavp
      421 sasggnqata papspagata papspagpsr vlfaqalqqg aapspqprav srgptpaaep
      481 rtqptaqpta qpsayrpsyg taaaagdanv aassprpasa aaavscsqpa aaalppsppc
      541 tppvavteqt peagdqatpg ddvrikeler tlpphfprrp ahgqlgrtiq llanhfriei
      601 ptgsvyhydv distetaqet kfpeqkkyrc lstkinrivi ellvkkyrqd lancipafdg
      661 rkklytrrql nfrertftvd leedqrsqkf iikiqyaatv nldalhgvfq krvqtvpqev
      721 lqaidivlrh spsinlapdg rsffrppgpn ehndlgggre vwfgyytsvr paqwkpmlnv
      781 dmsatafyds lplvdfmcrf fsdsrrvltp adlrslrdnq yarlnrelkg lrvkvthlpy
      841 prkykvvkit seaakdiyfe segsqisvad yfqsryrrls ypnfpcvqsg spthpvyipl
      901 evcelaegqh crkkleesqt aemikrtdtp pakrfleirq svrdmvstsd kylrefgvki
      961 npeptqvvgr ildppslvfe nnsmckprdg twdlrgqrfy kamsmtqwiv lnvshfaqkd
     1021 cldnfikmli rigqelgmri aqplavimfd tnrkpmrtvl teqrkqhpql emvvavitka
     1081 tnyaeikqva etelslrtqc ildnnvvqkc naaliqnlcq kinakmggin nsllmqekpk
     1141 llhrpvivig advshpspgd kirpsiaacv gsldsvpskf hatirvqied skakarveii
     1201 kdlkdmikel llvfyrttgh kplrivfyrd gvsegqflev rnhehgry
//
LOCUS       XP_077507389             266 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118148 [Amblyomma americanum].
ACCESSION   XP_077507389
VERSION     XP_077507389.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651263.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 22% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..266
                     /product="uncharacterized protein LOC144118148"
                     /calculated_mol_wt=30785
     CDS             1..266
                     /gene="LOC144118148"
                     /coded_by="XM_077651263.1:1..801"
                     /db_xref="GeneID:144118148"
ORIGIN      
        1 mdllkvpepl rlsgnlsenw qrfkqkfely lqatavekap ksdaskaall lsfagdeald
       61 ifnnfqygpg eskesygtvv qkfdayfsev sneiheryvf rtrrqmeket fekfvrdlkr
      121 qatqcnlgdq hdslirdqiv fgtnnsklre rmlaekdltl lkaeelckaa esaalwsqvw
      181 iksedcvdsi ssrtvstkke qqrdkklcrd nqqyccrkcn rtsskgwaas rfrtawssnq
      241 aqfqpfsrpv gfhwhfknly rknssa
//
LOCUS       XP_077507390             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118165 [Amblyomma americanum].
ACCESSION   XP_077507390
VERSION     XP_077507390.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651264.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144118165"
                     /calculated_mol_wt=14256
     CDS             1..133
                     /gene="LOC144118165"
                     /coded_by="XM_077651264.1:12..413"
                     /db_xref="GeneID:144118165"
ORIGIN      
        1 mcydvlssvk anedscavll sldeshdrcq lpasigpcvv pafacvippd lfmlktksci
       61 evvqatklrv icardlehag klvdgqwysl skasffsktk ttslgswamy letcgqdnae
      121 qgasasskks tkq
//
LOCUS       XP_077507391             363 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118181 [Amblyomma americanum].
ACCESSION   XP_077507391
VERSION     XP_077507391.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651265.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..363
                     /product="uncharacterized protein LOC144118181"
                     /calculated_mol_wt=40759
     Region          213..>320
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     CDS             1..363
                     /gene="LOC144118181"
                     /coded_by="XM_077651265.1:1..1092"
                     /db_xref="GeneID:144118181"
ORIGIN      
        1 mdpeespasg eaaaaaqttt tataqipreg elmailaais qnnerlgkmy arleavesqp
       61 htpsvrykhl gpkvpstsgk lssgktwcll rhlidplssk tatnrnltkv lntykvdghr
      121 lledlkakyl ktakgqypvs ernerpdnee lerqftmtel wraiddskkr sapgrdaity
      181 kllgnmsrta argllehine aweslrltke wkdaevrfip kpgkaltidn mrpisftscc
      241 vgkvvermvl rrlqkhleet dqmpekmygf rqhlstqdim iqlhglvlkp atrhaprgtl
      301 aldlkeafds ashesvlqsl nktrlldmga hntvekliea hfsnqiiwls hteqgravlr
      361 ktg
//
LOCUS       XP_077507392             111 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118212 [Amblyomma americanum].
ACCESSION   XP_077507392
VERSION     XP_077507392.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651266.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 33% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..111
                     /product="uncharacterized protein LOC144118212"
                     /calculated_mol_wt=12154
     Region          1..>61
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     CDS             1..111
                     /gene="LOC144118212"
                     /coded_by="XM_077651266.1:1..336"
                     /db_xref="GeneID:144118212"
ORIGIN      
        1 mdssgkipdg vlegtlvalg synecvdiva rdedqereyf rgqycaldik pflppkpkky
       61 npqhmfrnis evlpilkass lvlipapppa fcswqwdvaa vrdtlcssas a
//
LOCUS       XP_077507393             157 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118228 [Amblyomma americanum].
ACCESSION   XP_077507393
VERSION     XP_077507393.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651267.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..157
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..157
                     /product="uncharacterized protein LOC144118228"
                     /calculated_mol_wt=17190
     Region          <16..100
                     /region_name="AAT_I"
                     /note="Aspartate aminotransferase (AAT) superfamily (fold
                     type I) of pyridoxal phosphate (PLP)-dependent enzymes.
                     PLP combines with an alpha-amino acid to form a compound
                     called a Schiff base or aldimine intermediate, which
                     depending on the reaction, is the...; cl18945"
                     /db_xref="CDD:450240"
     CDS             1..157
                     /gene="LOC144118228"
                     /coded_by="XM_077651267.1:1..474"
                     /db_xref="GeneID:144118228"
ORIGIN      
        1 mhglaadvks sypqslacpi gslvagpkdf iekvarvrkc lgegmrqaei iataglvllr
       61 tivprlhegh ektqrlargv slkdnayisi dldtmqtnmv tereyedpqq aiivkmlpii
      121 ssqaravlyn dvnaddvdad vvkiryvide lcrsvda
//
LOCUS       XP_077507394             785 aa            linear   INV 25-NOV-2025
DEFINITION  neprilysin-like [Amblyomma americanum].
ACCESSION   XP_077507394
VERSION     XP_077507394.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651268.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..785
                     /product="neprilysin-like"
                     /calculated_mol_wt=86118
     Region          170..777
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..785
                     /gene="LOC144118276"
                     /coded_by="XM_077651268.1:1..2358"
                     /db_xref="GeneID:144118276"
ORIGIN      
        1 mrhsaecavf aaakpyhpag ilkapsvgrh tsaagqqavp dmgvvplpsw agtpdgtadr
       61 kcmpepespg agaagvqssd tsllthqgkk arfgvpslkl vspltltssr rgviilkmaa
      121 vlalltcavg atliiifwlr gdgppddmyc dsadcrhhea ligarlnrsi npcddfgafv
      181 csawmtssay hrgspstqqd vvdawsmrfe silqagltql kapgekafem frlckelpts
      241 apipaiealr kfmdnhnisw pqqplpsarp lallldlels wgvtlwfhvr nlrkqgkrer
      301 alivrpakri sdwlqlhrel vasggffdyw rrlfellapp gtasatenes rrvadvqtav
      361 lqklldagrt pspaqvllkd ieknitsips qewiallnkn lrteptfage driivthsal
      421 vdaldeitrt ygnmeivdsl awlfvqafap atdigllapa ranataaaag qhaafcaarv
      481 esayrfliis lftllnfsne erreidtrlr airgaaadkv aaagrldqls krraqeklne
      541 tttqlwpiae lldktaisim ytsfpdddts fvalwlrtlt slrglsrsyv hdetlylpan
      601 lalplldydy vlndirismq alsppvfylq gthamfyggl gflyasqlvr aldsrgmrig
      661 sdaqvaglwl sstwrhaatd vdsclgages hfpeipalev sygaleqalg qsddlhqrlr
      721 dgfterqlff itlcflmcsr pgtnpmgncn kavmnfppfa hefgcatnsk mkpakycpff
      781 dqgvm
//
LOCUS       XP_077507395             219 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118292 [Amblyomma americanum].
ACCESSION   XP_077507395
VERSION     XP_077507395.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651269.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..219
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..219
                     /product="uncharacterized protein LOC144118292"
                     /calculated_mol_wt=23833
     Region          6..174
                     /region_name="SGNH_hydrolase"
                     /note="or GDSL_hydrolase, is a diverse family of lipases
                     and esterases. The tertiary fold of the enzyme is
                     substantially different from that of the alpha/beta
                     hydrolase family and unique among all known hydrolases;
                     its active site closely resembles the typical...; cd00229"
                     /db_xref="CDD:238141"
     Site            order(11,36,64,155,158)
                     /site_type="active"
                     /db_xref="CDD:238141"
     Site            order(11,155,158)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238141"
     Site            order(11,36,64)
                     /site_type="active"
                     /note="oxyanion hole [active]"
                     /db_xref="CDD:238141"
     CDS             1..219
                     /gene="LOC144118292"
                     /coded_by="XM_077651269.1:1..660"
                     /db_xref="GeneID:144118292"
ORIGIN      
        1 mamrgvlvgd sqlkrlcrsr lrlcpsvetc tfsfggydav hlasavrrln lkpadfvily
       61 iggndlarpn aspqaicvnv kalvllllrd vapvvlvakv lprcfastda easrrllnrn
      121 ltaalkrmpc vgilnpdhrf ldamkapkrh lfaadgyhls agegieelae vfvkglakfy
      181 gagilapfsd aparyiihqc hqcgskghrn gdcyeycrp
//
LOCUS       XP_077507406             518 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118317 [Amblyomma americanum].
ACCESSION   XP_077507406
VERSION     XP_077507406.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651280.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 48% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..518
                     /product="uncharacterized protein LOC144118317"
                     /calculated_mol_wt=56943
     Region          <299..370
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     Region          <429..465
                     /region_name="zf-RVT"
                     /note="zinc-binding in reverse transcriptase; pfam13966"
                     /db_xref="CDD:433612"
     CDS             1..518
                     /gene="LOC144118317"
                     /coded_by="XM_077651280.1:1..1557"
                     /db_xref="GeneID:144118317"
ORIGIN      
        1 mpnitnvhln pnnfekmkvn yafhlfssev lrglflyree isksctyrep teqfitimqk
       61 lisvmtsrvp sealrknsee eavlqnaldy isrweantgp tkagflssst aeglrvtlkg
      121 tlelltylte cvgykyllts rlsqdplenl fgiirqfsgc idhptssqll tavnclsfyn
      181 lvkalntgnc agdaitslvg vneaanhadt lidkgkldea ssvldkselg dhiypqqtsd
      241 arltyylagy varkrvmgtk cpecfeqllt taedadeans taaelaglhl aadlllllpg
      301 qqpaviltds raalqllrqh qprqhtvanl tarlmaiqda grpvslqwlp shvgitknea
      361 adqlagaaht ngspvsskvt qldfarpalr qavralhpdp rvasgvrfmr vpdclprrer
      421 tlilrlrtgc swtqarlhrh grapspacsf cgadetlghl lcscpnleaa rrdmtagyrn
      481 lglpsysdqd llhpersqte afrllleflp stgsatrl
//
LOCUS       XP_077507446             394 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118366 [Amblyomma americanum].
ACCESSION   XP_077507446
VERSION     XP_077507446.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651320.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..394
                     /product="uncharacterized protein LOC144118366"
                     /calculated_mol_wt=44429
     CDS             1..394
                     /gene="LOC144118366"
                     /coded_by="XM_077651320.1:1..1185"
                     /db_xref="GeneID:144118366"
ORIGIN      
        1 mvsdfpleym hlvclgamrk llhcwvqrgs hkaklpaglq iaisgmlmgl qkyapqeiaq
       61 kprslceles wkatefinfl fyfgpavlkn niekcyynhy mklsvalfil acpalcqskl
      121 naagpllref vvdaeslyge giyvynihsl ghlpadvkky gyidsfsafr fesflgelkq
      181 dlksanrpll qivkrvyeqn elpracpvrq tslhkplgsg ltlprltgeq frkinlpdmv
      241 aevntahssl lvedgnvava tnvlsrdgdi ayaygrfftq lpnfhqgiad ttelhhykas
      301 gpsrvnavwp fqkvrskysy evarrralra qdqsdltsep qpvkrarrpp kqledftses
      361 dtskydsihk ssnddctefi tqpsqppapk ysed
//
LOCUS       XP_077507457             336 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118377 [Amblyomma americanum].
ACCESSION   XP_077507457
VERSION     XP_077507457.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651331.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..336
                     /product="uncharacterized protein LOC144118377"
                     /calculated_mol_wt=38102
     Region          14..98
                     /region_name="MADF_DNA_bdg"
                     /note="Alcohol dehydrogenase transcription factor
                     Myb/SANT-like; pfam10545"
                     /db_xref="CDD:463144"
     Site            order(264,266..267,270..271,274,290,292,296..297,300..301,
                     304,318,320,322,324..325,328..329,332)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          267..294
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          283..305
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          298..322
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          313..333
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..336
                     /gene="LOC144118377"
                     /coded_by="XM_077651331.1:87..1097"
                     /db_xref="GeneID:144118377"
ORIGIN      
        1 mgervwtnke eaqlirlysn arclwdrehp syadeskrrc ahdiiadsld nefsvpevve
       61 kiqelrgqyl kelkkesrrr sgkhfiyrwv hlermeflrs avtadereqa pgdvtllrpg
      121 qgvpggfhdt pdddtkgtaf pdlsshages tgssrkffsv papmmpevgr fpdqrqpaeg
      181 ldpivdesky avnvpatqll pvkaehdlll cenqerqslq eqqqhhteqk tpphvdtmth
      241 scsspngqga staigspnpe agpkrthfda hvrshtgekp yacdwpncgg kfarsgtlkr
      301 hyykhtgerr ylcdrcgrtf yrnehllghl kthkse
//
LOCUS       XP_077507480             318 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118407 [Amblyomma americanum].
ACCESSION   XP_077507480
VERSION     XP_077507480.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651354.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..318
                     /product="uncharacterized protein LOC144118407"
                     /calculated_mol_wt=36746
     CDS             1..318
                     /gene="LOC144118407"
                     /coded_by="XM_077651354.1:1..957"
                     /db_xref="GeneID:144118407"
ORIGIN      
        1 mifndeggeh riqeftlevv deykylgvwi nngaeylteh ekyvmnkvsr naavmknral
       61 wnynryvvlr giwkgvmvps ltfgnavlcm gsdvqarlei kqrgvgrlal gahgntpnqg
      121 vhghmgwasl esreasskia feerlrkige kqwakkvfgc lyirtvdtkw rkqlsvkkkv
      181 keteralwkt gmlrksalgt yrifkqeigk enisdncrgs flifearsga lrtktyrvry
      241 heidmlyigt lcvacgeeee tavhfilfcr glhpavessr adlpkalwfk dsegkvdfkr
      301 veetkrrlyd wwlkarhq
//
LOCUS       XP_077507490            3374 aa            linear   INV 25-NOV-2025
DEFINITION  protocadherin Fat 4-like [Amblyomma americanum].
ACCESSION   XP_077507490
VERSION     XP_077507490.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651364.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..3374
                     /product="protocadherin Fat 4-like"
                     /calculated_mol_wt=373825
     Region          56..153
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(59..60,116,119,149,151..152)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          161..263
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(168..169,225,227,260,262..263)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          298..379
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          388..488
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(394..395,447,449,484,486..487)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          498..576
                     /region_name="Cadherin"
                     /note="Cadherin domain; pfam00028"
                     /db_xref="CDD:394985"
     Region          609..700
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(616..617,667,669,697,699..700)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          785..869
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          878..980
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(885..886,939,944,976,978..979)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          991..1084
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(995..996,1048,1050,1081,1083..1084)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1093..1183
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          1193..1293
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1200..1201,1256,1258,1289,1291..1292)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1302..1391
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1308..1309,1357,1359,1387,1389..1390)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1400..1490
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          1505..1600
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1511..1512,1563,1565,1596,1598..1599)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1611..1696
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          1722..1806
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          1816..1916
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1823..1824,1877,1879,1912,1914..1915)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1925..2018
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1931..1932,1984,1986,2015,2017..2018)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2027..2130
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2034..2035,2089,2091,2127,2129..2130)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2140..>2216
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          2243..>2313
                     /region_name="CA_like"
                     /note="Cadherin repeat-like domain; cl46864"
                     /db_xref="CDD:481204"
     Region          2344..2445
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2351..2352,2410,2412,2441,2443..2444)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2453..2546
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2460..2461,2513,2515,2542,2544..2545)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2554..2653
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2561..2562,2617,2619,2650,2652..2653)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2662..2757
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2669..2670,2721,2723,2754,2756..2757)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2766..2851
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          2865..2981
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2871..2872,2922,2924,2977,2979..2980)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2991..3089
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     CDS             1..3374
                     /gene="LOC144118419"
                     /coded_by="XM_077651364.1:222..10346"
                     /db_xref="GeneID:144118419"
ORIGIN      
        1 maaaspqvpr cvrmrhqrtl wrwllasfal allhapgvhs qscqfyplge nrrfervsen
       61 iqvgqevfrv evhprrdlrv eamdnslsdi nyfrhedidd rtvsikvaks ledlvdrpdp
      121 qsvlkfkltc rgasgtdeaf lpvtvyiqdv ndhapefqnv pyhlevdelt pvgltvfrgv
      181 haidrdkpnt pnsdvtyaiv ggnennsfal sdplegiivi nkpldydhgp reyrleiqar
      241 dhgspesfqs vtsvtirvkd addqnpvftr qvykanvtea avitgarlrv klntdapvha
      301 fdqdlginap lrysiihgne lgifelqeqt aelfmvrevd lealpsptft lqvqatqmdn
      361 slryaltkve vlvtdindnv pvfdydmyni tvmenlppgf tvvqvhavdk dsgdnadfly
      421 hlvdetggfs idekegwirv rdpskldrek vdkltmrvla lerkpnvnpd akasttivev
      481 slldandnnp qflpegvyvf svtetdppgt vigsvvavdp dmgdngrvvf fkqndtlssk
      541 vpfgvypfng svyvldqfre iavkpdrytf fviasdvaks vherrtavav vhvnvtdvnn
      601 nvpefvgapy eahvgeslpa galvtqvsak dldantvlty siiagnddet fqmnaysgvi
      661 ttgtvldyek kqsydllvqv sdgfntavsp ltvhvvdind qqpvfthnyy nfsvveemtd
      721 nvtvgtirvs papssalsls lklhfpptsf rlvrwfsasl apkrvhcagd inalimlagt
      781 rthpqakdad lgdnavmkyr iiggnddgsf avnedtgavs tskrlnfetq peyalqvaaf
      841 nvkpfqgpna aimgnpvvnl ivkvqdvnde lvtferssyn friwenttrs etvgflnatn
      901 qhresgnqqv tfwiaegnaq gkfwinpstg elvlmdsvdr dppenqvqfr lkvfardlqs
      961 iapfntsvpv iidvldvndn aptfgeekfa lelpeslpvg telpafyrar didagpngqi
     1021 vrfmiegsed kffvnnmtga ltliseldye taetheftvf aidggtppkt gsasiiisvq
     1081 ninefspefi glpynfyvae naargtsvgq iqaqdkdgnk itytvsdgds effgieadtg
     1141 klyvrknles rteyalvvaa tddgspetrt lgvqvtvyvk evnnyppvfs hssykgtvle
     1201 kyatdrviik lfasdkdlqn ntitynissg nddniflidh qtgdirvnpa tvnlldydrk
     1261 kqfvmlvqak dshntplygl smvtidvlds ndhaptfsks aysaslpenl psghcflsih
     1321 adsgdsvdql tyeilnkdvp ftvnartgdv ctsqvldrer rdsyefavaa sdgkfdvkap
     1381 vsievvdend npprfekery tvsiphdsqa grsviqlhai dldvanngei tywiknthgi
     1441 feidaktglv rmaanlplna mrnlsyemev faqdhgaasn igktlllvrv ssthnsppvf
     1501 drfaysvsvd enaanvplvq vhasdpdpgs agkviyriar atrretfrid aqtgkitlva
     1561 pldyeqvkhl evfveardda aepqfattiv qvtvndvndn apeflsmprl lripettsqn
     1621 elvyrvqaid ldssaggnnd ikfelhppsq rfsihpktgq vfatqplqpg tellrivatd
     1681 ssiyplmsav emrvevykds vdeptpafsa vqytvdsasi ieagstvlea ratvpngspi
     1741 wynitgddgs hvrrfsinhd tgrivtttrl daekqsvyhf mvvavnrndp qhqaeagvvv
     1801 rltdknvrcp kfpfteyfas iqensppdti vlpnliaedv ekfgkisyqi tednsddnfm
     1861 ldtkqnlisl rvkkpldrds mptilqgvyt ltvtasnykc sgssrvkvfv edvndnspvf
     1921 eqndyvvele entppgqtvt hlsaadrdgl dlgkiryyii egnegedfsm dettgiisvr
     1981 iipdrektpd fmlkvlavds anntgrasvh ivikdtndwt ptflndtffl nvtegppsvg
     2041 ayvqlpvady ddginrqvel yivdgnnenq frlgvnenga vlrvvseldr dkykvpeaal
     2101 hlvivaardm gtpsrtgstt vavfiqdvnd sppqfekeay yefvsesasv gtvidtvkat
     2161 dpdspmntnm tysfargtsk rvpfmidpvl gtvnitrald isesqeyslt veandglwka
     2221 aapmkiyvse aeerdprfdq lhykfkvlen ipgaivgqve lqtrtirans qmrysiinte
     2281 vrpffnitkd geiftkvgld rekrskytft vmleekrptt kvsvsevsve vvdvndevpt
     2341 fmqsyqgtir ensppgtlvt vnptimavdn dsgnnsvvhy slggsgsdlf tildsgvvif
     2401 sggnpvtvld reladryell vtardlgnls sttyltikve dendnaptfq hgplrvllpe
     2461 tarpgarvaq vsaidadekg pnskveysiv sgargnvrid rqtgeifvvg alkpetvyll
     2521 nvsasdgrgl anlivvnitv ldvndhkptf vkpeysfsil egnykeqrar lgvlravded
     2581 rgkngavdya ivarkdqnlp fhvdvhtgel fatgildret kfkyyfhila ldngdppqns
     2641 tvnitvtved andekprfft dpylaqipen ldpgqkvtqi rafdpdtgen glvfyklggg
     2701 hdnkfyidsk dgtvwtlskl dfekqefynm tvvaydqgkp smsataklwv tvtdrndvvp
     2761 efpksvytle vaentrvgdv vytldagpgn flytldnpne vdtfsvepnt geirlakald
     2821 sihqshyrlv vsatddgdpp ksdhaevnii igsgqgvrlf plrmyevlvf enqlapqvil
     2881 dlnateeiah rpvqyaivgn nynglfaiep dtgrlsvtss ldreaipkyv vkvramnkkr
     2941 vgravresav akkgkkseee sahvafdeal vvvnvgdend nspvfdhqgk pivaavplea
     3001 sfgyqvtklh ardadtgfng airyeiiqkp edasskfqid pvtgvvrsvv tfsldggkmy
     3061 gfdvkavdre gseggnaava nvfvyvlpet klvlfvtdtp pvhveqhidr ilgylgnitg
     3121 fqvkmaklep hhedemehpe ssdlflyain kdtneivdtd tllnvlnens qvivryletf
     3181 rirriqgvsv qekisqmgtt eiaiialssv iflgavlgiv llcsscqrkk lrkrqktweq
     3241 qrlysiknpl mgkavanpyg ggrvsagppp igvppsnlqy nnvddvpmdy ndsitdtysv
     3301 nkhnrmklrq tapdgassln pprsprfery gknengwydn rdvetvksdm tgsnftswss
     3361 rrshqsrskp vtel
//
LOCUS       XP_077507505             500 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome P450 4V2-like [Amblyomma americanum].
ACCESSION   XP_077507505
VERSION     XP_077507505.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651379.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..500
                     /product="cytochrome P450 4V2-like"
                     /calculated_mol_wt=57050
     Region          112..490
                     /region_name="CYP4"
                     /note="cytochrome P450 family 4; cd20628"
                     /db_xref="CDD:410721"
     Site            order(143,160..161,168,172,179,346,349..350,353,415,419,
                     480..482,486..490)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410721"
     Site            order(161,348..349,353,418,420)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410721"
     CDS             1..500
                     /gene="LOC144118440"
                     /coded_by="XM_077651379.1:774..2276"
                     /db_xref="GeneID:144118440"
ORIGIN      
        1 mlsivastfs avssatsafs wryvmlltvt tatqrvllat iavaacvvvc qalhrfyrlw
       61 kvlrpmagpp dwfpplfnvn clraaaryrg ifntstglfs llvgltyffe rervfktylg
      121 fqpvvvlfkp eavesvlncs tnlkkpilys llnawlgtgl ltssgskwks rrkmltpafh
      181 friledflpi mneqgeifsq ilqhqthgvi ditqfvtact ldiicetamg vkvnaqtnps
      241 styvsniyrv gsnfinravr pwlwmdslyr ltrngrlyhh diraihtftk kvirerkdvk
      301 laekrlitds gdelkkrpmf ldvlldhhin ensiseedir eevdtfmfeg hdttsaaisw
      361 ciyllgqnpe aqkkvqdemd lifasdrnry atvadlkemk yleccikesl rlfpsvpiig
      421 reiykefcin grvvpegsiv iifsymlhrd rksfpqpeef rperflpens vgrhpfayvp
      481 fsagprncig ecaakistts
//
LOCUS       XP_077507517             277 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118449 [Amblyomma americanum].
ACCESSION   XP_077507517
VERSION     XP_077507517.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651391.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..277
                     /product="uncharacterized protein LOC144118449"
                     /calculated_mol_wt=30376
     Region          <77..255
                     /region_name="COG5025"
                     /note="Transcription factor of the Forkhead/HNF3 family
                     [Transcription]"
                     /db_xref="CDD:227358"
     Region          107..163
                     /region_name="FH_FOX"
                     /note="Forkhead (FH) domain found in Forkhead box (FOX)
                     family of transcription factors and similar proteins;
                     cl00061"
                     /db_xref="CDD:469596"
     CDS             1..277
                     /gene="LOC144118449"
                     /coded_by="XM_077651391.1:1..834"
                     /db_xref="GeneID:144118449"
ORIGIN      
        1 meplsaeveq etealsvayv ssimpdmstv vqaandvpss tgrqhrcdkp stkrrcgaal
       61 qstvrrrklp siedtctvdm appghlgell pmgtclrrps gflvqhiana ihksprrmlr
      121 vshiyealrn kfpffrfvdv daqatwkssv rhalyqnwfi kvpcsranns fwslnyserp
      181 rdwimpekee qgqplettea ltlasdlglv psaartppnl esprdirmss vptagvsqlp
      241 vvsslsqees stsnspqtvg taaavdagka evvslef
//
LOCUS       XP_077507525             111 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118459 [Amblyomma americanum].
ACCESSION   XP_077507525
VERSION     XP_077507525.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651399.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..111
                     /product="uncharacterized protein LOC144118459"
                     /calculated_mol_wt=12158
     CDS             1..111
                     /gene="LOC144118459"
                     /coded_by="XM_077651399.1:1..336"
                     /db_xref="GeneID:144118459"
ORIGIN      
        1 mctdlkdttt vkypgplpvq epaprpssap prlgtatsqq talalgvgrs reqhqlqaga
       61 qpqpqlqpqw qfvawphrna esfyllhvps lqdcaqqdmq pshgrqwpgd n
//
LOCUS       XP_077507534             277 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118469 [Amblyomma americanum].
ACCESSION   XP_077507534
VERSION     XP_077507534.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651408.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 11% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..277
                     /product="uncharacterized protein LOC144118469"
                     /calculated_mol_wt=30391
     Region          <77..255
                     /region_name="COG5025"
                     /note="Transcription factor of the Forkhead/HNF3 family
                     [Transcription]"
                     /db_xref="CDD:227358"
     Region          107..163
                     /region_name="FH_FOX"
                     /note="Forkhead (FH) domain found in Forkhead box (FOX)
                     family of transcription factors and similar proteins;
                     cl00061"
                     /db_xref="CDD:469596"
     CDS             1..277
                     /gene="LOC144118469"
                     /coded_by="XM_077651408.1:1..834"
                     /db_xref="GeneID:144118469"
ORIGIN      
        1 meplsaeieq etealsvayv ssimpdmstv vqaandvpss tgrqhrcdkp stkrrcgaal
       61 qstvrrrklp siedtctvdm appghlgell pmgtclrrps gflvqhiana ihksprrmlr
      121 vshiyealrn kfpffrfvdv daqatwkssv rhalyqnwfi kvpcsranns fwslnyserp
      181 rdwimpekee qgqplettea ltlasdlglv pstartppnv esprdirmss vptagasqlp
      241 vvsslsqees ttsnspqtvg taaavdagka kvvslef
//
LOCUS       XP_077507539             301 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118481 [Amblyomma americanum].
ACCESSION   XP_077507539
VERSION     XP_077507539.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651413.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 50% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..301
                     /product="uncharacterized protein LOC144118481"
                     /calculated_mol_wt=32770
     Region          <101..279
                     /region_name="COG5025"
                     /note="Transcription factor of the Forkhead/HNF3 family
                     [Transcription]"
                     /db_xref="CDD:227358"
     Region          131..205
                     /region_name="Forkhead"
                     /note="Forkhead domain; pfam00250"
                     /db_xref="CDD:459732"
     CDS             1..301
                     /gene="LOC144118481"
                     /coded_by="XM_077651413.1:61..966"
                     /db_xref="GeneID:144118481"
ORIGIN      
        1 mqapemtdsd tvttamhhes gaadmeqlsa eveqeteals vayvssimpd mstvaqaand
       61 vpssagrqhr cdkpstkrrc gaalqstvrr rklpgsedtc tvdmappgpl gellpmgtcl
      121 rrpsgflvqh ianaihkspr rmlrvshiye alrnkfpfyr fldvdaqatw kssvrhalyq
      181 nwfikvpcsr aknsfwslny serprdwimp ekeeqgqple ttealtlasd lglvpsaart
      241 ppnvesprdi rmssvptaga sqlpvvssls qeesstsnsp qtvgtaaavd agkaevvsle
      301 f
//
LOCUS       XP_077507545             198 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118491 [Amblyomma americanum].
ACCESSION   XP_077507545
VERSION     XP_077507545.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651419.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..198
                     /product="uncharacterized protein LOC144118491"
                     /calculated_mol_wt=21770
     Region          28..102
                     /region_name="Forkhead"
                     /note="Forkhead domain; pfam00250"
                     /db_xref="CDD:459732"
     CDS             1..198
                     /gene="LOC144118491"
                     /coded_by="XM_077651419.1:64..660"
                     /db_xref="GeneID:144118491"
ORIGIN      
        1 mappghlgel lpmgtclrrp sgflvqhian aihksprrml rvshiyealr nkfpffrfvd
       61 vdaqatwkss vrhalyqnwf ikvpcsrann sfwslnyser prdwimpeke eqgqplette
      121 altlasdlgl vpsaartppn lesprdirms svptagvsql pvvsslsqee sstsnspqtv
      181 gtaaavdagk aevvslef
//
LOCUS       XP_077507552             277 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118501 [Amblyomma americanum].
ACCESSION   XP_077507552
VERSION     XP_077507552.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651426.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..277
                     /product="uncharacterized protein LOC144118501"
                     /calculated_mol_wt=30392
     Region          <77..255
                     /region_name="COG5025"
                     /note="Transcription factor of the Forkhead/HNF3 family
                     [Transcription]"
                     /db_xref="CDD:227358"
     Region          107..163
                     /region_name="FH_FOX"
                     /note="Forkhead (FH) domain found in Forkhead box (FOX)
                     family of transcription factors and similar proteins;
                     cl00061"
                     /db_xref="CDD:469596"
     CDS             1..277
                     /gene="LOC144118501"
                     /coded_by="XM_077651426.1:1..834"
                     /db_xref="GeneID:144118501"
ORIGIN      
        1 meplsaeieq etealsvayv ssimpdmstv vqaandvpss tgrqhrcdkp stkrrcgaal
       61 qstvrrrklp siedtctvdm appghlgell pmgtclrrps gflvqhiana ihksprrmlr
      121 vshiyealrn kfpffrfvdv daqatwkssv rhalyqnwfi kvpcsranns fwslnyserp
      181 rdwimpekee qgqplettea ltlasdlglv pstartppnv esprdirmss vptagasqlp
      241 vvsslsqees ttsnspqtvg taaavdagka evvslef
//
LOCUS       XP_077507562             301 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118511 [Amblyomma americanum].
ACCESSION   XP_077507562
VERSION     XP_077507562.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651436.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 27% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..301
                     /product="uncharacterized protein LOC144118511"
                     /calculated_mol_wt=32770
     Region          <101..279
                     /region_name="COG5025"
                     /note="Transcription factor of the Forkhead/HNF3 family
                     [Transcription]"
                     /db_xref="CDD:227358"
     Region          131..205
                     /region_name="Forkhead"
                     /note="Forkhead domain; pfam00250"
                     /db_xref="CDD:459732"
     CDS             1..301
                     /gene="LOC144118511"
                     /coded_by="XM_077651436.1:54..959"
                     /db_xref="GeneID:144118511"
ORIGIN      
        1 mqapemtdsd tvttamhhes gaadmeqlsa eveqeteals vayvssimpd mstvaqaand
       61 vpssagrqhr cdkpstkrrc gaalqstvrr rklpgsedtc tvdmappgpl gellpmgtcl
      121 rrpsgflvqh ianaihkspr rmlrvshiye alrnkfpfyr fldvdaqatw kssvrhalyq
      181 nwfikvpcsr aknsfwslny serprdwimp ekeeqgqple ttealtlasd lglvpsaart
      241 ppnvesprdi rmssvptaga sqlpvvssls qeesstsnsp qtvgtaaavd agkaevvsle
      301 f
//
LOCUS       XP_077507578             231 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118533 [Amblyomma americanum].
ACCESSION   XP_077507578
VERSION     XP_077507578.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651452.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..231
                     /product="uncharacterized protein LOC144118533"
                     /calculated_mol_wt=26173
     Region          73..161
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     CDS             1..231
                     /gene="LOC144118533"
                     /coded_by="XM_077651452.1:1..696"
                     /db_xref="GeneID:144118533"
ORIGIN      
        1 mpmaytvaek gskevrlrta gyekqaadqs pcviihllty tgyhdstgan eyldrlphyh
       61 qvtgiadaam larvvpvslt eqasawyrla gnrartmeef casfrdeflp anyewslrre
      121 lelrtqhpde slleyvramd elyrladppa tntdkvervt rqahpifray lrgdrfrdld
      181 elaaeakriq gdiltarayr ppppaslsle lrcawngssa rspsrreasa r
//
LOCUS       XP_077507598             236 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118563 [Amblyomma americanum].
ACCESSION   XP_077507598
VERSION     XP_077507598.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651472.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 15% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..236
                     /product="uncharacterized protein LOC144118563"
                     /calculated_mol_wt=24940
     CDS             1..236
                     /gene="LOC144118563"
                     /coded_by="XM_077651472.1:51..761"
                     /db_xref="GeneID:144118563"
ORIGIN      
        1 mpsprpslpe rsatpavgnv ssslepvspe tyytpqsggt ptdlsisatf lsplpetycp
       61 psqssglppe gisspislsp ipgpfqhrtw gvaptplpry ssgvyppyat pplsaqsmyg
      121 srsgysgsgy srsdyagpvs gmpyppttpn tapardaayy etspryydgt vagescyapg
      181 kgtlkthmnf nvqqdvealk kamrgwivir aqrdentpld pvrahndaav ssiill
//
LOCUS       XP_077507607             493 aa            linear   INV 25-NOV-2025
DEFINITION  annexin-B12-like [Amblyomma americanum].
ACCESSION   XP_077507607
VERSION     XP_077507607.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651481.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..493
                     /product="annexin-B12-like"
                     /calculated_mol_wt=53611
     Region          <62..214
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          245..310
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          317..382
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          401..466
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     CDS             1..493
                     /gene="LOC144118574"
                     /coded_by="XM_077651481.1:140..1621"
                     /db_xref="GeneID:144118574"
ORIGIN      
        1 msgssggasg gynpgcapgy ppgypptsss syptdavrpr gfvdpeeppr rmpsprpsfp
       61 ersatpavgn vssslepvsp etyytpqssg tptdlsisat flsplpetyc ppsqssglpp
      121 egisspisls pipgpfqhrt wgvaptplpr yssgvyppya tpplsaqsmy gsrsgysgsg
      181 ysrsdyagpv sgmpyppttp ntapardaay yetspryydg tvtgtscyap gkgtlkthmn
      241 fnvqqdveal kkamrgwttd haaiidilca rsepqrqeiv ttykqsfgrd lvedakssfr
      301 gdlediivgl lyplheylar elrkaiaglg tdeeclveil ctqsnqdirl ikdhyqrifk
      361 kdlekdvigd tsgafrrllv smcnaqreen tpldpvrarn daaelhkagv qrwgtdvsaf
      421 nriiatqsye qlrlvfreyn avanhtvmea iksemsgdlk ngflaivkcv ysppfyfaet
      481 lyhamkrsge asv
//
LOCUS       XP_077507613             325 aa            linear   INV 25-NOV-2025
DEFINITION  annexin-B12-like [Amblyomma americanum].
ACCESSION   XP_077507613
VERSION     XP_077507613.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651487.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..325
                     /product="annexin-B12-like"
                     /calculated_mol_wt=36249
     Region          77..142
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          149..214
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          233..298
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     CDS             1..325
                     /gene="LOC144118586"
                     /coded_by="XM_077651487.1:274..1251"
                     /db_xref="GeneID:144118586"
ORIGIN      
        1 mygsrsgysg sgysrsdyag pvsgmpyppt tpntaparda ayyetspryy dgtvtgtscy
       61 apgkgtlkth mnfnvqqdve alkkamrgwt tdhaaiidil carsepqrqe ivttykqsfg
      121 rdlvedakss frgdlediiv gllyplheyl arelrkaiag lgtdeeclve ilctqsnqdi
      181 rlikdhyqri fkkdlekdvi gdtsgafrrl lvsmcnaqre entpldpvra rndaaelhka
      241 gvqrwgtdvs afnriiatqs yeqlrlvfre ynavanhtvm eaiksemsgd lkngflaivk
      301 cvysppfyfa etlyhamkrs geasv
//
LOCUS       XP_077507620             594 aa            linear   INV 25-NOV-2025
DEFINITION  annexin A4-like [Amblyomma americanum].
ACCESSION   XP_077507620
VERSION     XP_077507620.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651494.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 11% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..594
                     /product="annexin A4-like"
                     /calculated_mol_wt=64857
     Region          <62..214
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          245..310
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          317..382
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          401..466
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          476..>527
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     CDS             1..594
                     /gene="LOC144118597"
                     /coded_by="XM_077651494.1:165..1949"
                     /db_xref="GeneID:144118597"
ORIGIN      
        1 msgssggasg gynpgcapgy ppgypptsss syptdavrpr gfvdpeeppr rmpsprpsfp
       61 epsvtpavgn vssslepvsp etyytpqsgg aptelsisat flsplpetyc ppsqssglpp
      121 egisspisls pipgpfqhrt wgvaptplpr yssgvyppya tpplsaqsmy gsrsgysgsg
      181 ysrsdyagpv sgmpyhpttp ntapardaay yetspryydd tvagascyap gkgtlkthmn
      241 fnvqqdveal kkamrgwttd haaiidilca rsepqrqeiv ttykqsfgrd lvedakcsfr
      301 gdlediiegl lyplheylar elrkaiaglg tdeeclveil ctqsnqdirl ikdhyqrifk
      361 kdlekdvigd tsgafrrllv smcnaqrden tpldpvrarn daaelhkagv qrwgtdvsaf
      421 nriiatqsye qlrlvfreyh avanhtvmea iksemsgdlk ngflaivkcv ysppfyfaet
      481 lyhamkglgt ndrvlkrvvv srcekdleli kqeyldkykv slasaiqatt traganlqet
      541 vavkaeatlq atttiqqnaa lplcfsplst sqsypqrypq ggtyqvqmry ppvr
//
LOCUS       XP_077507628             512 aa            linear   INV 25-NOV-2025
DEFINITION  annexin-B12-like [Amblyomma americanum].
ACCESSION   XP_077507628
VERSION     XP_077507628.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651502.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..512
                     /product="annexin-B12-like"
                     /calculated_mol_wt=55776
     Region          39..>175
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          212..277
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          284..349
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          368..433
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          443..508
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     CDS             1..512
                     /gene="LOC144118608"
                     /coded_by="XM_077651502.1:161..1699"
                     /db_xref="GeneID:144118608"
ORIGIN      
        1 msgssggasg gynpgcapgy ppgypptsss syptdavrpr gfvdpeeppr lmpstkpsfp
       61 eksptpavgn vssslepvsp etyytpqsgg aptelsisat flsplpetyc ppsqssglpp
      121 egisspisls pipgpsqhrt wgvaptplpr sdyagpvsgm pyppttpnta pardaayyet
      181 spryydgtva gascyapgkg tlkthmnfnv qqdvealkka mrgwttdqaa iidilcarse
      241 pqrqeivtty kqsfgrdlve daksafrgdl ediiegllyp lheylarelr kaiaglgtde
      301 eclveilctq snqdirlikd hyqrifkkdl ekdvigdtsg afrrllvsmc naqrdentpl
      361 dpvrarndaa elhkagvqrw gtdvsafnri iatqsyeqlr lvfreynava nhtvmeaiks
      421 emsgdlkngf laivkcvysp pfyfaetlyh amkgigtndr vlkrvvvsrc ekdlelikqe
      481 yldkynvsla saiqsdtsgh yrkallaivq gn
//
LOCUS       XP_077507632             351 aa            linear   INV 25-NOV-2025
DEFINITION  annexin-B12-like [Amblyomma americanum].
ACCESSION   XP_077507632
VERSION     XP_077507632.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651506.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 26% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..351
                     /product="annexin-B12-like"
                     /calculated_mol_wt=39359
     Region          26..77
                     /region_name="ANX"
                     /note="Annexin repeats; smart00335"
                     /db_xref="CDD:197661"
     Region          84..159
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          178..243
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          253..325
                     /region_name="Annexin"
                     /note="cl02574"
                     /db_xref="CDD:413385"
     CDS             1..351
                     /gene="LOC144118620"
                     /coded_by="XM_077651506.1:1..1056"
                     /db_xref="GeneID:144118620"
ORIGIN      
        1 mneegeidde ediidkspdf ydspttdhga tidilcarse pqrqeivtty kqsfgrdlee
       61 daksalrgsl ediilgllyp lheylarelr kaiaglgkde eclieilctq gnqlghpahq
      121 gpltensdsl sghfvfkkdw ekdvigatsg dvrrllvsmc nakrdentpl dpvrarndaa
      181 elhkagvqrw gtdvsafnri iatqsyeqlr lvfreynava nhtvmeaiks emsgdlknsf
      241 laivkcvysp pfyfaetlyh arkryrehst qdslvdlsqg lgtndrvlkr vvvsrcekdl
      301 elikqeyldk ykvslasaiq ltasgavvef rgrqdrkpvq tdaiedggak y
//
LOCUS       XP_077507646             462 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118642 [Amblyomma americanum].
ACCESSION   XP_077507646
VERSION     XP_077507646.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651520.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..462
                     /product="uncharacterized protein LOC144118642"
                     /calculated_mol_wt=51476
     Region          78..206
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     CDS             1..462
                     /gene="LOC144118642"
                     /coded_by="XM_077651520.1:295..1683"
                     /db_xref="GeneID:144118642"
ORIGIN      
        1 mgkarnsrlp llsyvvahlm aaaegvyett tsaagaavnq appqsfeadm dealsllskk
       61 vlplvsefvg saelsgacts slikfflhlr lkepwavrmv tanglvptnl legslvslgg
      121 yeqclktrfr gyegevlfkg ryctlfselp kdamrgfver fqaagmlrgr rnpltattdt
      181 afqsidirvg icvpslcsqs evdflfssim rqygvnatvr gcrtddpkpl tllqacsial
      241 fsslalllcv gtitewyler qdptkpskka vaapaavlcw fsvasntrrl fdvsyyegtp
      301 rqnllffsga klilcfwvvf fhsytliqpe fyhsgfkifd lgdrvyfqlv tnaflsissl
      361 ffiqgftfsy mvnvgdqell pkkclrsylh ilvhryvrlt vpvvlvvffa fllpamaegp
      421 adqelfgkqi drcaknwwtv vthmnnfnal netasiriyt fa
//
LOCUS       XP_077507652             546 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118654 [Amblyomma americanum].
ACCESSION   XP_077507652
VERSION     XP_077507652.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651526.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 11% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..546
                     /product="uncharacterized protein LOC144118654"
                     /calculated_mol_wt=56455
     CDS             1..546
                     /gene="LOC144118654"
                     /coded_by="XM_077651526.1:147..1787"
                     /db_xref="GeneID:144118654"
ORIGIN      
        1 mgwpsaaaam lpllvamlcv avrcpaaevt qqpeqfdstr vttarpeaaa klraddgknd
       61 vqerpvtsgv eledyddnad elpgadlktt sgstvptdes ddddddydst ddfedvetgp
      121 teaseattap apttaktanv iaaaeenvvt ksstdektka aanatdvsvv qttqasvtta
      181 stpsttaatt tttsteapss vvvmatsspt aststegttt lkmdstsdvq sttavaestt
      241 sgksttenpd vttavkdgsd vtaksgiese ftgdnqiaay egaasslsvt avesttsstt
      301 ttepsttess ttestttest ttestttest ttestttett ttettsestt tettttesst
      361 tpstedstta gatsesddse tkttssdste tastqsgseg taaggareme dldgadgprp
      421 ttespaeptt vrdyepdlna vinqfmtqil plttrflsdt nvsadcvnhl wkvyqgfrrq
      481 dawalrsrrd gglvqqrkgk pcvgrsspia snnarvdrlg salphavrcf etcvdlcgpl
      541 gageer
//
LOCUS       XP_077507661             115 aa            linear   INV 25-NOV-2025
DEFINITION  transcription factor Adf-1-like [Amblyomma americanum].
ACCESSION   XP_077507661
VERSION     XP_077507661.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651535.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..115
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..115
                     /product="transcription factor Adf-1-like"
                     /calculated_mol_wt=13576
     Region          10..109
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     CDS             1..115
                     /gene="LOC144118663"
                     /coded_by="XM_077651535.1:1..348"
                     /db_xref="GeneID:144118663"
ORIGIN      
        1 maamqefner lieqvqeerv lwdmsythyk sqqrrnvawr riaatlgstv pdvkarwknl
       61 rdtfrrlyka rnptprsgap aedededcfd gddsatkwff ydrllflrdt vtgrp
//
LOCUS       XP_077507662             328 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118676 [Amblyomma americanum].
ACCESSION   XP_077507662
VERSION     XP_077507662.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651536.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..328
                     /product="uncharacterized protein LOC144118676"
                     /calculated_mol_wt=36715
     Region          9..94
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..328
                     /gene="LOC144118676"
                     /coded_by="XM_077651536.1:1..987"
                     /db_xref="GeneID:144118676"
ORIGIN      
        1 madddggscr snydggpsvr vykfpadetq rtawtkaisc edfvptkhtv vcekhfqtsd
       61 fvrfatytde nsgnvievpl qrvrlkpaav psvfpgcpky lsqppssrea paekkarlea
      121 aslrkaiels vaaqeeedrr nkicsfgelc salpklktsd fwtvvttqak vlflelsldq
      181 apavhssvvv lddlsvqvfl ggsrlhnigd eyvpsqmkdl reldkvlqfv eklqscasgs
      241 ekehqllatv lmlldevnkd yelqehhgwn lqvlkfvrsq velvlknvpr yppdvlvfsg
      301 llytisphay rfirsslkik lphpdtir
//
LOCUS       XP_077507686             199 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118712 [Amblyomma americanum].
ACCESSION   XP_077507686
VERSION     XP_077507686.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651560.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 32% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..199
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..199
                     /product="uncharacterized protein LOC144118712"
                     /calculated_mol_wt=22053
     CDS             1..199
                     /gene="LOC144118712"
                     /coded_by="XM_077651560.1:1..600"
                     /db_xref="GeneID:144118712"
ORIGIN      
        1 msrqrqpsss vaagqrpreq sgsqsivhdv passvnayrd mavllrvvav lldaenvasv
       61 vlvfkvltrh fdeprkaqfe drrrrqlnrr lsatlkrlsg vhilnpehrf ldasgkpmls
      121 lfaadryhva rdrgidqmsk iivaalvkiy gpgiasasra rpgevyvvhr crrcgakghk
      181 tdhcwaycsp rrhaaagrg
//
LOCUS       XP_077507711             254 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118738 [Amblyomma americanum].
ACCESSION   XP_077507711
VERSION     XP_077507711.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651585.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..254
                     /product="uncharacterized protein LOC144118738"
                     /calculated_mol_wt=26553
     CDS             1..254
                     /gene="LOC144118738"
                     /coded_by="XM_077651585.1:91..855"
                     /db_xref="GeneID:144118738"
ORIGIN      
        1 msqhgsmagg enkrasekaa vtvastvkav asrgkavala grndklarvp ktairmkrqa
       61 vrnarpgetl vslagspvks qpvetpvhvh idnaevvtla cdassresvk nvckaiqems
      121 vdeaamadeh gemsqhgsma ggenkrasek aavtvastvk avasrgrava lagrndklar
      181 vpktairmkr qavrnarpge tlvslagspv ksepvetpvh vhidnvevvt lacdassres
      241 vqnvckaiqe mcek
//
LOCUS       XP_077507719             209 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118749, partial [Amblyomma
            americanum].
ACCESSION   XP_077507719
VERSION     XP_077507719.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651593.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 21% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         <1..209
                     /product="uncharacterized protein LOC144118749"
     CDS             1..209
                     /gene="LOC144118749"
                     /coded_by="XM_077651593.1:<1..630"
                     /db_xref="GeneID:144118749"
ORIGIN      
        1 hwfvdgafsv apavfyqlyt ihalqsgrvl rlaycllkrk ttatytrifr kmlelepals
       61 pmslltdfeq gaiqafrsev fdyfertyig vafgrnrtrk dtpfppthws chdhvldglp
      121 ktnnsveawh dslqqslgfr hpdpyrliaa lkqeqslteq kqaeagrwna vsqraayaki
      181 aerlqgvlan ysttpliavm lgcrftyel
//
LOCUS       XP_077507720             216 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118760 [Amblyomma americanum].
ACCESSION   XP_077507720
VERSION     XP_077507720.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651594.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 47% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..216
                     /product="uncharacterized protein LOC144118760"
                     /calculated_mol_wt=23572
     CDS             1..216
                     /gene="LOC144118760"
                     /coded_by="XM_077651594.1:1..651"
                     /db_xref="GeneID:144118760"
ORIGIN      
        1 merrgrngkr srkerspfcv vafvdegdsv aavptkwlsr skdtcmwppk gfgsysvkql
       61 vrrlakptde wrvcpcrvlv yadtygearq kaaiaeqtsh ldsseesede avkghdgvla
      121 mptlhsgkhv dyhhlnsacn sdvestpqdt vshnaaldca gssswnldsa phhhadehap
      181 dgipiirtrt pmgasgflss qhalgvegqd ghyiee
//
LOCUS       XP_077507748             351 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118810 [Amblyomma americanum].
ACCESSION   XP_077507748
VERSION     XP_077507748.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651622.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 11% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..351
                     /product="uncharacterized protein LOC144118810"
                     /calculated_mol_wt=37154
     Region          <36..209
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          <269..>351
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..351
                     /gene="LOC144118810"
                     /coded_by="XM_077651622.1:1..1056"
                     /db_xref="GeneID:144118810"
ORIGIN      
        1 mpvccafgcs trsgkgkrlf svpldgfpss lphnsppvsp raqegirqpl allatssvqv
       61 patpkplpgq sllrpkaaap alglsqqfel avpatpkllp gqsllrpkaa apalglsqqf
      121 elavpatpkl lsgqsllrpk aaapalglsp qfelevpatp kllpgqsllr pkaaapalgl
      181 spqfelavpa tpkplpgqsl lkpkaaipav rsqhfdlaga dcdalkdevr dlkrklslaq
      241 rkvsslenen knlrqgisdy lnddqgysva relgiplpse rtlqrkiegf kfvpgilsev
      301 idllrakmgq lsgqechavl mydemqltks ldfdsstnml lgrlspsqva v
//
LOCUS       XP_077507753             655 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-4-like [Amblyomma americanum].
ACCESSION   XP_077507753
VERSION     XP_077507753.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651627.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..655
                     /product="protein argonaute-4-like"
                     /calculated_mol_wt=74448
     Region          108..>655
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     CDS             1..655
                     /gene="LOC144118821"
                     /coded_by="XM_077651627.1:1..1968"
                     /db_xref="GeneID:144118821"
ORIGIN      
        1 mntfsrhggl fldenniseh lsvkaagnie gfvdlgdyts sdhkevvgts ragaganvta
       61 fitrgggtag ngarivapte ssptrspstt aapmragncv gntdsqndel kiqeiertlp
      121 shfprrpaeg kvgrpiqltg nhfnieipsg nvfhydveic setrnearvp ekrkyrcist
      181 kinrlviell akkyrvdlng cmpafdgrkn lytrrelkfr ersltvdfee dqrnqkfivk
      241 iqyaaavnld allavfdkrv shvphevlqa mdivlrhgps ikptpvgrsf fkppspndyn
      301 ilgggreawf gyytsvrpaq wkpmlnvdms atdfyeplpv tdfmcelfie grremsardf
      361 kelrdfqnvr lnkelkglrv kvthlpyprk ykvvlvtkep akqlffdmed gsccsvadyf
      421 qnryhrlryp nlpciqtgna arpvylplev cvivegqhck kkldenqtse mikhtpqppa
      481 krfneirqsv wdlpatlsrw tllnlsrfaq rdsldnfvkm lirvgqelgm rieqpldvtt
      541 tdanrkpvrn ilpeeqrrta slemvisvla kntnyaeikq kinvkldgin nsllpkempk
      601 mfqkpviiig admddsasts rlevikdlkv mmkdmlkafy hatkhkperi iffrd
//
LOCUS       XP_077507761             398 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118831 [Amblyomma americanum].
ACCESSION   XP_077507761
VERSION     XP_077507761.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651635.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 37% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..398
                     /product="uncharacterized protein LOC144118831"
                     /calculated_mol_wt=42268
     Region          <201..>246
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     Region          240..>381
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..398
                     /gene="LOC144118831"
                     /coded_by="XM_077651635.1:33..1229"
                     /db_xref="GeneID:144118831"
ORIGIN      
        1 msshgaaiaa vagrgnrfas dadkdyeivl ptlptgrvvl ntvflhgdvr arpyrvedfr
       61 dalanagalp dvvalgayqi nhvwavtfst adaakklaat kelevkgrrc ivfdpedhqv
      121 klrlhwmlhg vadedirtaf aafgnvtevt rerwrvagmk ekgsttrtvl lklkpgvkvd
      181 dlphqvrvag elalvvvpgr pmqclrcggt ghvrrdckvp rcskcrrygh seadcvrtya
      241 satghvaasv saelmdvvea eeaasgaddt gkaegvaesg spatdpsstp vetpkdgass
      301 sahverkaas eshdekddsr mnvgvttpak rphaetkadg ekavepsvge ptaktpqarr
      361 aglrprpkvp aekrggdkap pdqehvlapd dtggdgiv
//
LOCUS       XP_077507796             153 aa            linear   INV 25-NOV-2025
DEFINITION  superkiller complex protein 2-like [Amblyomma americanum].
ACCESSION   XP_077507796
VERSION     XP_077507796.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651670.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..153
                     /product="superkiller complex protein 2-like"
                     /calculated_mol_wt=17225
     Region          <1..54
                     /region_name="rRNA_proc-arch"
                     /note="rRNA-processing arch domain; pfam13234"
                     /db_xref="CDD:463813"
     Region          80..>141
                     /region_name="DSHCT"
                     /note="DSHCT (NUC185) domain; pfam08148"
                     /db_xref="CDD:462374"
     CDS             1..153
                     /gene="LOC144118884"
                     /coded_by="XM_077651670.1:110..571"
                     /db_xref="GeneID:144118884"
ORIGIN      
        1 metvdlvkrs qqleekllks kcltcplfea hfeqehrrqr lseevrhlqh qlsedslasm
       61 pdyrnhvlal erlgyvepsg tltlkgrvar slssyevmls dlllqesllt lgapevaglf
      121 sclvyeqrsn seavvprsvk avsnccllrq vsq
//
LOCUS       XP_077507804             140 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118892 [Amblyomma americanum].
ACCESSION   XP_077507804
VERSION     XP_077507804.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651678.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..140
                     /product="uncharacterized protein LOC144118892"
                     /calculated_mol_wt=15152
     CDS             1..140
                     /gene="LOC144118892"
                     /coded_by="XM_077651678.1:293..715"
                     /db_xref="GeneID:144118892"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrppfa altsvlccqr pasigpcvlp afgcvippdp
       61 fmlktkscme vvqatklrvi fardleqagk lvdgqwyslt ktktiflgsw amylgtcgqd
      121 nagqhasass kkkhetvnny
//
LOCUS       XP_077507811             119 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118904 [Amblyomma americanum].
ACCESSION   XP_077507811
VERSION     XP_077507811.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651685.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..119
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..119
                     /product="uncharacterized protein LOC144118904"
                     /calculated_mol_wt=12957
     CDS             1..119
                     /gene="LOC144118904"
                     /coded_by="XM_077651685.1:1..360"
                     /db_xref="GeneID:144118904"
ORIGIN      
        1 mhalcaqgls flalillsgr lyeaskeiaf lrlvlaawip seadlmlgga addhkslvld
       61 rsrqtltnlk gdleafllel tsarttlals vvfviymlsg wwmvfalkqa kdnvsvvhp
//
LOCUS       XP_077507820             564 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077507820
VERSION     XP_077507820.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651694.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 64% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..564
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=63125
     Region          29..390
                     /region_name="Piwi-like"
                     /note="PIWI domain. Domain found in proteins involved in
                     RNA silencing. RNA silencing refers to a group of related
                     gene-silencing mechanisms mediated by short RNA molecules,
                     including siRNAs, miRNAs, and heterochromatin-related
                     guide RNAs. The central component...; cl00628"
                     /db_xref="CDD:412485"
     Site            order(144,148,160..163,166,167,171)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(198,200,241,381)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..564
                     /gene="LOC144118925"
                     /coded_by="XM_077651694.1:1..1695"
                     /db_xref="GeneID:144118925"
ORIGIN      
        1 mikhtaqppa krfneirqsv qdlvsssdqc lrkfgikist kpaqlrgrvl dppslvfens
       61 siashatrwt llnlsrfaqr dsldnfvkml irigqelgmr ieqpldvttt nanrkpvrni
      121 lpeeqrrtas lemvisvlak ntnyaeikqv aeteiglgtq cvmdnnkinv kldginnsll
      181 pkekpkmfqk pviiigadme ksattsrlei ikdlkdmmkd mlkafyratr hkperiiffp
      241 dgvsegqfme vrnrevsair lacqelspne tyepaltfiv vqkrhhmrfm pandrdgvgr
      301 crnvppgttv dsavahpfdf dfylcshfcl qgtsrpshyy ivwddssfta delqklcyyl
      361 chtyascsrs vsipapvyya hlaayrarnh vmskvdvtss ssdssggsad svfisqyfea
      421 vkvletlqna ctsceeahcr smprynacvg ascthetlsr krwspesnll eaggpprsvp
      481 gsrvmlhvml grtfhvmlgr tlvkpmwlls pdhevglaip vpgvvcpmah davgftcywv
      541 lcgddemsdl rgqpssppgr levn
//
LOCUS       XP_077507836             422 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118947 [Amblyomma americanum].
ACCESSION   XP_077507836
VERSION     XP_077507836.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651710.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..422
                     /product="uncharacterized protein LOC144118947"
                     /calculated_mol_wt=47980
     Region          208..370
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..422
                     /gene="LOC144118947"
                     /coded_by="XM_077651710.1:1..1269"
                     /db_xref="GeneID:144118947"
ORIGIN      
        1 mahskpvpda vdrgcgflam faacqtemqq rrlrqlaild eleerqaeqe qaertcrrlq
       61 alaarlcsre rsvwtypret swyettlphl pesgfrenfr mtrstfqyiv gvcecmkrrd
      121 tnmrkaiplr krvaiaiyrl atsaeertva nvfgvsrssv nnivrefcdt mvevlepryi
      181 kmprthelae hlrqfaavag fpqgvgavdg chievcpple havdyhnykg wystillava
      241 dhrykflytn sgspgrnhda avfevsrlpk ildsdlfkre vreiegvqig plfladqafp
      301 lqknvmkpfp hsgiigsdaq efnfrlssar rvvenafgrl karfrillkg lecdivnvpy
      361 viraccvlhn iceeltdscd snwldvvhve drrrpqpvct ssqvepsgva vrnalakhla
      421 sk
//
LOCUS       XP_077507854             404 aa            linear   INV 25-NOV-2025
DEFINITION  degenerin deg-1-like [Amblyomma americanum].
ACCESSION   XP_077507854
VERSION     XP_077507854.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651728.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..404
                     /product="degenerin deg-1-like"
                     /calculated_mol_wt=45544
     Region          6..385
                     /region_name="ASC"
                     /note="Amiloride-sensitive sodium channel; pfam00858"
                     /db_xref="CDD:459966"
     CDS             1..404
                     /gene="LOC144118966"
                     /coded_by="XM_077651728.1:1..1215"
                     /db_xref="GeneID:144118966"
ORIGIN      
        1 mspagalvai gclvgflyqs qrvitsylry dfsvvqwevs rmgiefpavt vclddwmstd
       61 rlcnytrgkc skkdmtityg vfhpqyipdl rihgaylpqe ifqcsfased prcepfdcvs
      121 slkrtyfrpp tqqcytvdaw ndshfrqcps pwswelqlya swdpqntmvf fdtgslatvv
      181 hqartcpvdk ltavalqpgk lltvavsqrr vqrlpspyks kcsdyiargi ypafegylny
      241 dssayiwrpd gqscmqhcsm elerercgcv rpqhefagav gwtncqlqes gacfhalnas
      301 gaytscerrc glpceeivyd vklsgiteeg gfkhdrknsv glevkfssdv qqvvqyvpvi
      361 trvevlgyls gylgiwlgls latvalqahk rfcpahwpss terp
//
LOCUS       XP_077507864             169 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118975 [Amblyomma americanum].
ACCESSION   XP_077507864
VERSION     XP_077507864.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651738.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..169
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..169
                     /product="uncharacterized protein LOC144118975"
                     /calculated_mol_wt=17223
     CDS             1..169
                     /gene="LOC144118975"
                     /coded_by="XM_077651738.1:90..599"
                     /db_xref="GeneID:144118975"
ORIGIN      
        1 mlgqpkqksk eedarkkrdr hktgggsaqc tvsaqseqvi avashimtrv gnqtdsdggi
       61 dlppvanlpv irllqpmvdg agneefhyae ddwepplvde pqspaaavgk sgatpeaieq
      121 esnlffpavq padasstaaa gsssatvasg aaadpttaea vatgspfqt
//
LOCUS       XP_077507872             146 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144118985 [Amblyomma americanum].
ACCESSION   XP_077507872
VERSION     XP_077507872.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651746.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..146
                     /product="uncharacterized protein LOC144118985"
                     /calculated_mol_wt=15787
     CDS             1..146
                     /gene="LOC144118985"
                     /coded_by="XM_077651746.1:216..656"
                     /db_xref="GeneID:144118985"
ORIGIN      
        1 mnrvaavvpa ilrarnssqa rdpthrppfa altsvlccqr pasigpcvlp afgcvippdp
       61 fmlktkscme vvqatklrvi fardleqagk lvdgqwysls kasffsktkt iflgswamyl
      121 gtcgqdnagq hasasskkkh etvnky
//
LOCUS       XP_077507884             129 aa            linear   INV 25-NOV-2025
DEFINITION  histone-lysine N-methyltransferase set-17-like [Amblyomma
            americanum].
ACCESSION   XP_077507884
VERSION     XP_077507884.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651758.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..129
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..129
                     /product="histone-lysine N-methyltransferase set-17-like"
                     /calculated_mol_wt=14109
     Region          3..111
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(5..6,61..66,77..79,100..103)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Site            order(5..6,62..66,102)
                     /site_type="other"
                     /note="SAM binding site [polypeptide binding]"
                     /db_xref="CDD:380914"
     CDS             1..129
                     /gene="LOC144119004"
                     /coded_by="XM_077651758.1:1..390"
                     /db_xref="GeneID:144119004"
ORIGIN      
        1 mtgaqygvft leplpkrvyf gpyegvkmkd ngegnsytwq vhkngevflv dglpldksnw
       61 mryvnyaagp eeqnlvaytr ggniyyrtik avgtgqelfv wngtssareq gllakpcssg
      121 lstkalfaa
//
LOCUS       XP_077507891             183 aa            linear   INV 25-NOV-2025
DEFINITION  histone-lysine N-methyltransferase PRDM7-like [Amblyomma
            americanum].
ACCESSION   XP_077507891
VERSION     XP_077507891.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651765.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 16% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="histone-lysine N-methyltransferase PRDM7-like"
                     /calculated_mol_wt=19954
     Region          <6..32
                     /region_name="zf_PR_Knuckle"
                     /note="PR zinc knuckle motif; pfam18445"
                     /db_xref="CDD:375871"
     Region          41..165
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(59..60,115..120,131..133,154..157)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Site            order(59..60,116..120,156)
                     /site_type="other"
                     /note="SAM binding site [polypeptide binding]"
                     /db_xref="CDD:380914"
     CDS             1..183
                     /gene="LOC144119012"
                     /coded_by="XM_077651765.1:1..552"
                     /db_xref="GeneID:144119012"
ORIGIN      
        1 melakfcyec gadhpgdcpv hgpwthvkdt kvvagdplra nktlpkglsi rrstmtgaqy
       61 gvftleplpk gvyfgpyegv kmkdngegns ytwqvhknge vflldgrpld ksnwmryvny
      121 aagpeeqnlv aytrggniyy rtikavgtgq elfvwngtss areqgllakp cssglstkal
      181 faa
//
LOCUS       XP_077507894             195 aa            linear   INV 25-NOV-2025
DEFINITION  histone-lysine N-methyltransferase PRDM7-like [Amblyomma
            americanum].
ACCESSION   XP_077507894
VERSION     XP_077507894.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651768.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 57% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..195
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..195
                     /product="histone-lysine N-methyltransferase PRDM7-like"
                     /calculated_mol_wt=21171
     Region          <16..41
                     /region_name="zf_PR_Knuckle"
                     /note="PR zinc knuckle motif; pfam18445"
                     /db_xref="CDD:375871"
     Region          50..177
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(68..69,127..132,143..145,166..169)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Site            order(68..69,128..132,168)
                     /site_type="other"
                     /note="SAM binding site [polypeptide binding]"
                     /db_xref="CDD:380914"
     CDS             1..195
                     /gene="LOC144119023"
                     /coded_by="XM_077651768.1:1..588"
                     /db_xref="GeneID:144119023"
ORIGIN      
        1 melaknlgvd ailtvcyecg adhpgdcpvh gpwthvkdtk vvagdplran ktlpkglsir
       61 rstmtgaqyg vftleplpkg vyfgpyegvk mkdngegnsy twqvrlvhkn gevflldgrp
      121 ldksnwmryv nyaagpeeqn lvaytrggni yyrtikavgt gqelfvwngt ssareqglla
      181 kpcssglstk alfaa
//
LOCUS       XP_077507912             160 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119056 [Amblyomma americanum].
ACCESSION   XP_077507912
VERSION     XP_077507912.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651786.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..160
                     /product="uncharacterized protein LOC144119056"
                     /calculated_mol_wt=17823
     Region          <85..122
                     /region_name="Dimer_Tnp_hAT"
                     /note="hAT family C-terminal dimerization region;
                     pfam05699"
                     /db_xref="CDD:399013"
     CDS             1..160
                     /gene="LOC144119056"
                     /coded_by="XM_077651786.1:1..483"
                     /db_xref="GeneID:144119056"
ORIGIN      
        1 meqlvkledi ltsaavcsry tadeleaalg vhaanfdlcr lsaqfmllst llngadsttt
       61 lgileilqre sqnirelmdq vvryaqllfs ipasvasger sfsalrriit ylrnrmtqkr
      121 lshllishih kermallcld dvirefvsrt aeraatfgpa
//
LOCUS       XP_077507920             237 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119066 [Amblyomma americanum].
ACCESSION   XP_077507920
VERSION     XP_077507920.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651794.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..237
                     /product="uncharacterized protein LOC144119066"
                     /calculated_mol_wt=26424
     Region          70..226
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..237
                     /gene="LOC144119066"
                     /coded_by="XM_077651794.1:141..854"
                     /db_xref="GeneID:144119066"
ORIGIN      
        1 mrlglfekdl adrfcastat vsrigitwas faftkltqip lwmskehvqk emplsfqgky
       61 satrvilhvt eikcqaassl alqsatfsly ksantfkgli gispngtvtf vsnlfpgsis
      121 dkecvsksgf lafpfndgdd vmadkgfkie dmlktinval nnppflrrgq fseeeikete
      181 eiaslrihvq rriqhiksfh ifdrlvplsl gpiinemwiv cailtnfqsp lvqlsde
//
LOCUS       XP_077507943             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119099 [Amblyomma americanum].
ACCESSION   XP_077507943
VERSION     XP_077507943.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651817.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144119099"
                     /calculated_mol_wt=15306
     CDS             1..141
                     /gene="LOC144119099"
                     /coded_by="XM_077651817.1:141..566"
                     /db_xref="GeneID:144119099"
ORIGIN      
        1 mtpepesaaq agnlvgrhri gdvmkllvrr tearlsaaye giatncrhpv qhypshehly
       61 lgdaflaani iswaitsvfd lpavitqptl dmfgfplsll chdggwstpv aeqpagcsgl
      121 ryarrrnhga tiqrhavtdh s
//
LOCUS       XP_077507959             680 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119128 [Amblyomma americanum].
ACCESSION   XP_077507959
VERSION     XP_077507959.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651833.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..680
                     /product="uncharacterized protein LOC144119128"
                     /calculated_mol_wt=73025
     Region          31..297
                     /region_name="RT_nLTR_like"
                     /note="RT_nLTR: Non-LTR (long terminal repeat)
                     retrotransposon and non-LTR retrovirus reverse
                     transcriptase (RT). This subfamily contains both non-LTR
                     retrotransposons and non-LTR retrovirus RTs. RTs catalyze
                     the conversion of single-stranded RNA into...; cd01650"
                     /db_xref="CDD:238827"
     Site            order(121..126,180..181,213,215..216,293..294)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238827"
     Site            order(121..126,180,215)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238827"
     Site            181
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238827"
     Region          483..583
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     Site            order(486..488,502..504,507,531,561,579)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260008"
     CDS             1..680
                     /gene="LOC144119128"
                     /coded_by="XM_077651833.1:1..2043"
                     /db_xref="GeneID:144119128"
ORIGIN      
        1 mtyaaqltai clamsagrse klpvlpesws taivvpirka rkpltafssy rpvsltsaac
       61 ksmefvalqr lswmacacgf lsecqtgfrr hrctadsiad vvscleeakq lgdvallvll
      121 dvqaafdslp hssieqalde lgvtgrlrqf vtaflsnrsm qvrvggtlss prpittgvpq
      181 gsvlspflfn lvmaglpsai qqglchrvfc svytddvalw vrasrrhits asqalqrald
      241 evqcflvsrs lvlsatksea lfvhprcara ssrictlrid gtdlpwrpqv rylgltidhc
      301 ltwipadkpl llhldrvgra aehllargng csstwafrlf eaagtsavly alplatlttt
      361 rlrrllgvpq ssqitatlae agalplnlil lqrglthtdr lrhvpdgral rarphsrmgm
      421 lyaaymeslg ppashpalpp pptrpplqvs ldfggmskrh tpaaalnqta aafldemkgt
      481 tllftdgsln atrtcrlpfp gtssaaelag lhlaadliat ippgsvvvlc dswpalqlls
      541 rpldnvattl edaghqislh wvpghsgieg ndladtlaks ahtdgspvsy avtqhdfarp
      601 limqmvralh sdrriarrqa lppalgpaaq aglspppapp hrlhvdalqv vrvwqgplsc
      661 lpllrghgda wpppagvpdp
//
LOCUS       XP_077508028             212 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077508028
VERSION     XP_077508028.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651902.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..212
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=23579
     Region          <1..192
                     /region_name="Piwi-like"
                     /note="PIWI domain. Domain found in proteins involved in
                     RNA silencing. RNA silencing refers to a group of related
                     gene-silencing mechanisms mediated by short RNA molecules,
                     including siRNAs, miRNAs, and heterochromatin-related
                     guide RNAs. The central component...; cl00628"
                     /db_xref="CDD:412485"
     Site            order(12,16,28..31,34,43,46,50,54)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     CDS             1..212
                     /gene="LOC144119228"
                     /coded_by="XM_077651902.1:1..639"
                     /db_xref="GeneID:144119228"
ORIGIN      
        1 mviivltknt nyaeikqvae teiglrtqcv mdnnvikkrn aalitnlcqk inakldgins
       61 llpkeqpkif qkpviiigad vthpapgdkl rlsiaahkpe riiffrdgvs egqfmevsai
      121 rlacqelspn etyepaltft vvqkrhhtrf mptndrdgvg qcrnvppgtt vdsvvthpld
      181 fdfylcshfg nqvhsngmps gapsvnsryw wl
//
LOCUS       XP_077508038             322 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-4-like [Amblyomma americanum].
ACCESSION   XP_077508038
VERSION     XP_077508038.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651912.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..322
                     /product="protein argonaute-4-like"
                     /calculated_mol_wt=37225
     Region          2..>322
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     CDS             1..322
                     /gene="LOC144119239"
                     /coded_by="XM_077651912.1:1..969"
                     /db_xref="GeneID:144119239"
ORIGIN      
        1 msqeiertlp shfpcrpaqa gklgrpiqlt anyfnieipa gnvfyydvei csetrkeakv
       61 penrkyrcis tkinrlviel lvkkyrvdln gcmpafdgrk nlytrrepkf rtrtftvdfe
      121 enqriqkfiv kiqyagtvnl dalhavfdkc vsqvpqavdi vlrhgpsikl tpvgrpffkp
      181 pspndyntlg ggrevwfgyy tsvrpaqwkp mldvdmsapa fyepllhfre lrdsqnvrln
      241 kelkvlrvkv thlpcprkyk vvrvtkepak klffnmedgs rcsvadyfyk ryhrlsypnl
      301 pciqtgsath pvylplevcv iv
//
LOCUS       XP_077508044             802 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 2-like [Amblyomma americanum].
ACCESSION   XP_077508044
VERSION     XP_077508044.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651918.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..802
                     /product="endothelin-converting enzyme 2-like"
                     /calculated_mol_wt=87610
     Region          93..726
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..802
                     /gene="LOC144119249"
                     /coded_by="XM_077651918.1:1..2409"
                     /db_xref="GeneID:144119249"
ORIGIN      
        1 magegckaps ttsggldrrq ravalghlls sllgvaltls aitiglsvlr hqrgsegsgv
       61 fgmgrsswnt cnsklcmahf rrlkdslnrs tapcgdfyrf acgawhpraa ksetvfrdll
      121 avaqqdairy leavndasnk mvlptgkvll pdsrpvlrin raerahqvcl hsgsstkdaa
      181 lltefladrs lswperttap aqpldvlldl dinwnlglwf svrvspipgc nqrqvrvgsg
      241 ivsydwkral dtmvtrgvyk arvrrfhdal rarsfaamsd kgmkelrrye stivgalcgs
      301 vsggqnelaf plkqmqnlat prlaaaqwdt llnwqlnkal rvteadrvia tsvkllqavd
      361 yllsslpaev vlhqlawslv qmigwmadsa lpgrptayem edmrsadcfw vthrafgvth
      421 lsgyinttyp lhwlssvdai lmtitqtain fflktswiha esraaavdkl rrmntslwps
      481 dsylnaseva ailenfpepr edgavlrywl dalsarrall grqwdvadaf ypgdlqppra
      541 lfryhyyrne lavslhalrs plfvegvgla vnmgglgahy avalarafdp rgvlldgrgs
      601 tsglwwgkss yrdyerrtac pataasgaeg gvqafegvaa ieiaygafna srsiadqgtr
      661 thqrrriflg lsedqaffva fceascargp serqqlsctl plknfrefat aftcpvaspm
      721 npalrcgffe asdqraarkn ggesedagks astnasanvg adgavtswtr pparrsgsdd
      781 qdpltlattg laghmarqpp vl
//
LOCUS       XP_077508053             767 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 2-like [Amblyomma americanum].
ACCESSION   XP_077508053
VERSION     XP_077508053.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651927.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..767
                     /product="endothelin-converting enzyme 2-like"
                     /calculated_mol_wt=83939
     Region          93..726
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..767
                     /gene="LOC144119256"
                     /coded_by="XM_077651927.1:1..2304"
                     /db_xref="GeneID:144119256"
ORIGIN      
        1 magegckaps ttsggldrrq ravalghlls sllgvaltls aitiglsvlr hqrgsegsgv
       61 fgmgrsswnt cnsklcmahf rrlkdslnrs tapcgdfyrf acgawhpraa ksetvfrdll
      121 avaqqdairy leavndasnk mvlptgkvll pdsrpvlrin raerahqvcl hsgsstkdaa
      181 lltefladrs lswperttap aqpldvlldl dinwnlglwf svrvspipgc nqrqvrvgsg
      241 ivsydwkral dtmvtrgvyk arvrrfhdal rarsfaamsd kemkelrrye stivgalcgs
      301 vsggqnelaf plkqmqnlat prlaaaqwdt llnwqlnkal rvteadrvia tsvkllqavd
      361 yllsslpaev vlhhlawslv qmigwmadsa lpgrptayem edmrsadcfw vthrafgvth
      421 lsgyinttyp lhwlssvdai lmtitqtain fflktswiha esraaavdkl rrmntslwps
      481 dsylnaseva ailenfpepr edgavlrywl dalsarrall grqwdvadaf ypgdlqppra
      541 lfryhyyrne lavslhalrs plfvegvgla vnmgglgahy avalarafdp rgvlldgrgs
      601 tsglwwgkss yrdyerrtac pataasgaeg gvqafegvaa ieiaygafna srsiadqgtr
      661 thqrrriflg lsedqaffva fceascargp serqqlsctl plknfrefat aftcpvaspm
      721 npalrcgffe asdqraarkn ggesedagks astnasanvg adgavts
//
LOCUS       XP_077508065             802 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 2-like [Amblyomma americanum].
ACCESSION   XP_077508065
VERSION     XP_077508065.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651939.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..802
                     /product="endothelin-converting enzyme 2-like"
                     /calculated_mol_wt=87816
     Region          93..726
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..802
                     /gene="LOC144119266"
                     /coded_by="XM_077651939.1:1..2409"
                     /db_xref="GeneID:144119266"
ORIGIN      
        1 magegckaps ttsggldrrq ravalghlls sllgvaltls aitiglsvlr hqrgsegsgv
       61 fgmgrsswnt cnsklcmahf rrlkdslnrs tapcgdfyrf acgawhpraa ksetvfrdll
      121 avaqqdairy leavnddsnk mvlptgkvll pdsrpvlrin raerahqvcl hsgsstkdaa
      181 lltefladrs lswperttap aqpldvlldl dinwnlglwf svrvspipgc nqrqvrvgsg
      241 ivsynwkral dtmvtrgvyk arvrrfhdal rarsfaamsd kemkelrrye stivgalcgs
      301 vsggqnelaf plkqmqnlat prlaaaqwdt llnwqlnkal rvteadrvia tsvkllqavd
      361 yllsslpaev vlhqlawslv qmigwmadsa lpgrptayem edmrsadcfw vthrafgvth
      421 lsgyinttyp lhwlssvdai lmtitqtain fflktswiha esraaavdkl rrmntslwpc
      481 dsylnaseva ailenfpepr edgavlrywl dalsarrall grqwdvadaf ypgdlqppra
      541 lfryhyyrne lavslhalrs plfvegvgla vnmgglgahy avalarafdp rsvlldgrgs
      601 tsglwwgkss yrdyerrtac pataasgaeg gvqafegvaa ieiaygafna srsiadqgtr
      661 thqrrriflg lsedqaffva fceascargp serqqlsctl plknfrefat aftcpvaspm
      721 npalrcgffe asdqraarkn ggesedagks astnasanvg adgavtswtr pparrsgsdd
      781 qdpltlastg laghmvrqpk vl
//
LOCUS       XP_077508072             802 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 2-like [Amblyomma americanum].
ACCESSION   XP_077508072
VERSION     XP_077508072.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651946.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..802
                     /product="endothelin-converting enzyme 2-like"
                     /calculated_mol_wt=87858
     Region          93..726
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..802
                     /gene="LOC144119278"
                     /coded_by="XM_077651946.1:1..2409"
                     /db_xref="GeneID:144119278"
ORIGIN      
        1 magegckaps ttsggldrrq ravalghlls sllgvaltls aitiglsvlr hqrgsegsgv
       61 frmgrnswnt cnsklcmahf rrlkdslnrs tapcgdfyrf acgawhpraa ksetvfrdll
      121 avaqqdairy lesvndasnk mvlptgkvll pdsrpvlrin raerahqvcl hsgsstkdaa
      181 lltefladrs lswperttap aqplgvlldl dinwnlglwf svrvspipgc nqrqvrvgsg
      241 ivsydwkral dtmvtrgvyk arvrrfhdal rarsfaamsd kemkelrrye stivgalcgs
      301 vsggqnelaf plkqmqnlat prlaatqwdt llnwqlnkal rvteadrvia tsvkllqavd
      361 yllsslpaev vlhqlawslv qmigwmadsa lpgrptayem edmrsadcfw vthrafgvth
      421 lsgyinttyp lhwlssvdai lmtitqtain fflktswiha esraaavdkl rrmntslwps
      481 dsylnaseva ailenfpepr edgavlrywl dalsarrall grqwdvadaf ypgdlqppra
      541 lfryhyyrne lavslhalrs plfvegvgla vnmgglgahy avalarafdp rgvlldgrgs
      601 tsglwwgkss yrdyerrtac pataasgaeg gvqafegvaa ieiaygafna srsiadqgtr
      661 thqrrriffg lsedqaffva fceascargp serqqlsctl plknfrefat aftcpvaspm
      721 npalrcgffe asdqraarkn ggesedagks astnasanvg adgavtswtr pparrsgsdd
      781 qdpltlattg laghmvrqpp vl
//
LOCUS       XP_077508078             802 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 2-like [Amblyomma americanum].
ACCESSION   XP_077508078
VERSION     XP_077508078.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651952.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..802
                     /product="endothelin-converting enzyme 2-like"
                     /calculated_mol_wt=87925
     Region          93..726
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..802
                     /gene="LOC144119288"
                     /coded_by="XM_077651952.1:1..2409"
                     /db_xref="GeneID:144119288"
ORIGIN      
        1 magegckaps ttsggldrrq ravalghlls sllgvaltls aitiglsvlr hqrgsegsgv
       61 frmgrnswnt cnsklcmahf rrlkdslnrs tapcgdfyrf acgawhpraa ksetvfrdll
      121 avaqqdairy lesvndasnk mvlptgkvll pdsrpvlrin raerahqvcl hsgsstkdaa
      181 lltefladrs lswperttap aqplgvlldl dinwnlglwf svrvspipgc nqrqvrvgsg
      241 ivsydwkral dtmvtrgvyk arvrrfhdal rarsfaamsd kerkelrrye stivgalcgs
      301 vsggqnelaf plkqmqnlat prlaatqwdt llnwqlnkal rvteadrvia tsvkllqavd
      361 yllsslpaev vlhqlawslv qmigwmadsa lpgrptayem edmrsadcfw vthrafgvth
      421 lsgyinttyp lhwlssvdai lmtitqtain fflktswiha esraaavdkl rrmntslwps
      481 dsylnaseva ailenfpepr edgavlrywl dalsarrall grqwdvadaf ypgdlqppra
      541 lfryhyyrne lavslhalrs plfvegvgla vnmgglgahy avalarafdp rgvlldgrgs
      601 tsglwwgkss yrdyerrtac pataasgaeg gvqafegvaa ieiaygafna srsiadqgtr
      661 thqrrriffg lsedqaffva fceascargp serqqlsctl plknfrefat aftcpvaspm
      721 npplrcgffe asdqraarkn ggesedagks sstnasanvg adgavtswtr pparrsgsdd
      781 qdpltlattg laghmvrqpp vl
//
LOCUS       XP_077508088             421 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 1-like [Amblyomma americanum].
ACCESSION   XP_077508088
VERSION     XP_077508088.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651962.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..421
                     /product="endothelin-converting enzyme 1-like"
                     /calculated_mol_wt=45808
     Region          <23..345
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..421
                     /gene="LOC144119298"
                     /coded_by="XM_077651962.1:1..1266"
                     /db_xref="GeneID:144119298"
ORIGIN      
        1 migwtadsal pgrptayeme dmrsadcfwv thrafgvthl sgyinttypl hwrssvdail
       61 mtitqtainf flktswihae sraaavdklr rmntslwpsd sylnasevaa ilekfpepre
      121 dgavlrywld alsarrallg rqwdvadafy pgdlqppral fryhyylnel avslhalrsp
      181 lfvegvglav nmgglgahya valarafdpr gvlldgrgst sglwwgkssy rdyerrtacp
      241 ataasgaegg vqafegvaai eiaygafnas rsiayqgtrt hqrrriflgl sedqgffvaf
      301 cqascargps erqqlsctlp lknfrefata ftcpvaspmn palrcgffea sdqraarkng
      361 gesedagksa stnasanvga dgavtswtrp parrsgsddq dpltlattgl aghmvrqppv
      421 l
//
LOCUS       XP_077508098             802 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 2-like [Amblyomma americanum].
ACCESSION   XP_077508098
VERSION     XP_077508098.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651972.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..802
                     /product="endothelin-converting enzyme 2-like"
                     /calculated_mol_wt=87760
     Region          93..726
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..802
                     /gene="LOC144119307"
                     /coded_by="XM_077651972.1:1..2409"
                     /db_xref="GeneID:144119307"
ORIGIN      
        1 magegckaps ttsggldrrq rvvalghlls sllgvaltls aitiglsvlr hqrgsegsgv
       61 fgmgrsswnt cnsklcmahf rrlkdslnrs tapcgdlyrf acgawhpraa ksetvfrdll
      121 avaqqdairy leavndasnk mvlptgkvll pdsrpvlrin raerahqvcl hsgsstkdaa
      181 lltefladrs lswperttap aqpldvlldl dinwnlglwf svrvspipgc nqrqvrvgsg
      241 ivsydwkral dtmvtrgvyk arvrrfhdal rarsfaamsd kemkelrrye stivgalcgs
      301 vsggqnelaf plkqmqnlat prlaaaqwdt llnwqlnkal rvteadrvia tsvkllqavd
      361 yllsslpaev vlhhlawslv qmigwmadsa lpgrptayem edmrsadcfw vthrafgvth
      421 lsgyinttyp lhwrssvdai lmtitqtaik fflktswiha esraaavdkl rrmntslwps
      481 dsylnaseva ailenfpepr edgavlrywl dalsarralf grqwdvadaf ypgdlqppra
      541 lfryhyylne lavslhalrs plfvegvgla vnmgglgahy avalarafdp rgvlldgrgs
      601 tsglwwgkss yrdyerrtac pataasgaeg gvqafegvaa ieiaygafna srsiadqgtr
      661 thqrrriflg lsedqgffva fcqascargp serqqlsctl plknirefat aftcpvaspm
      721 npalrcgffe atdqraarkn ggesedagks astnasanvg adgavtswtr pparrsgsdd
      781 qdpftlattg laghmvrqpp vl
//
LOCUS       XP_077508105             802 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 2-like [Amblyomma americanum].
ACCESSION   XP_077508105
VERSION     XP_077508105.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651979.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..802
                     /product="endothelin-converting enzyme 2-like"
                     /calculated_mol_wt=87864
     Region          93..726
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..802
                     /gene="LOC144119318"
                     /coded_by="XM_077651979.1:1..2409"
                     /db_xref="GeneID:144119318"
ORIGIN      
        1 magegckaps ttsggldrrq ravalghlls sllgvaltls aitiglsvlr hqrgsegsgv
       61 frmgrnswnt cnsklcmahf rrlkdslnrs tapcgdfyrf acgawhpraa ksetvfrdll
      121 avaqqdairy lesvndasnk mvlptgkvll pdsrpvlrin raerahqvcl hsgsstkdaa
      181 lltefladrs lswperttap aqplgvlldl dinwnlglwf svrvspipgc nqrqvrvgsg
      241 ivsydwkral dtmvtrgvyk arvrrfhdal rarsfaamsd kemkelrrye stivgalcgs
      301 vsggqnelaf plkqmqnlat prlaatqwdt llnwqlnkal rvteadrvia tsvkllqavd
      361 yllsslpaev vlhqlewslv qmigwmadsa lpgrptayem edmrsancfw vthrafgvth
      421 lsgyinttyp lhwlssvdai lmtitqtain fflktswiha esraaavdkl rrmntslwps
      481 dsylnaseva ailenfpepr edgavlrywl dalsarrall grqwdvadaf ypgdlqppra
      541 lfryhyyrne lavslhalrs plfvegvgla vnmgglgahy avalarafdp rgvlldgrgs
      601 tsglwwgkss yrdyerrtac pataasgaeg gvqafegvaa ieiaygafna srsiadqgtr
      661 thqrrriflg lsedqaffva fceascargp serqqlsctl plenfrefat aftcpvaspm
      721 npalrcgffe asdqraarkn ggesedagks astnasanvg adgavtswtr pparrsgsdd
      781 qdpltlattg laghlvrqpp vl
//
LOCUS       XP_077508107             802 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 2-like [Amblyomma americanum].
ACCESSION   XP_077508107
VERSION     XP_077508107.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651981.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..802
                     /product="endothelin-converting enzyme 2-like"
                     /calculated_mol_wt=87866
     Region          93..726
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..802
                     /gene="LOC144119330"
                     /coded_by="XM_077651981.1:1..2409"
                     /db_xref="GeneID:144119330"
ORIGIN      
        1 magegckaps ttsggldrrq ravalghlls sllgvaltls aitiglsvlr hqrgsegsgv
       61 frmgrnswnt cnsklcmahf rrlkdslnrs tapcgdfyrf acgawhpraa ksetvfrdll
      121 avaqqdairy lesvndasnk mvlptgkvll pdsrpvlrin raerahqvcl hsgsstkdaa
      181 lltefladrs lswperttap aqplgvildl dinwnlglwf svrvspipgc nqrqvrvgsg
      241 ivsydwkral dtmvtrgvyk arvrrfhdal rarsfaamsd kemkelrrye stivgalcgs
      301 vsggqnelaf plkqmqnlat prlaatqwdt llnwqlnkal rvteadrvia tsvkllqavd
      361 yllsslpaev vlhqlawslv qmigwmadsa lpgrptayem edmrsadcfw vthrafgvth
      421 lsgyinttyp lhwlssvdai lmtitqtain fflktswiha esraaavdkl rrmntslwps
      481 dsylnaseva ailenfpepr edgavlrywl dalsarrall grqwdvadaf ypgdlqppra
      541 lfryhyyrne lavslhalrs plfvegvgla vnmgglgahy avalarafdp rgvlldgrgs
      601 tsglwwgkss yrdyerrtac pataasgaeg gvqafegvaa ieiaygafna srsiadqgtr
      661 thqrrriflg lsedqaffva fceascargp serqqlsctl plknfrefat aftcpvaspm
      721 npalrcgffe asdqraarkn ggesedagks astnasanvv adgavtswtr pparrsgsdd
      781 qdpltlattg laghmvrqpp vl
//
LOCUS       XP_077508117             802 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 2-like [Amblyomma americanum].
ACCESSION   XP_077508117
VERSION     XP_077508117.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651991.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..802
                     /product="endothelin-converting enzyme 2-like"
                     /calculated_mol_wt=87803
     Region          93..726
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..802
                     /gene="LOC144119344"
                     /coded_by="XM_077651991.1:1..2409"
                     /db_xref="GeneID:144119344"
ORIGIN      
        1 magegckaps ttsggldrrq savalghlls sllgvaltls aitiglsvlr hqrgsegsgv
       61 fgmgrsswnt cnsklcmahf rrlkdslnrs tapcgdfyrf acgawhpraa ksdsvfrdll
      121 avaqqdairy leavndasnk mvlptgkvll pdsrpvlrin raerahqvcl hsgsstkdaa
      181 lltefladrs lswperttep aqpldvlldl dinwnlglwf svrvspipgc nqrqvrvgsg
      241 ivsydwkral dtmvtrgvyk arvrrfhdal rarsfaamsd kemkelrrye stivgalcgs
      301 vsggqnelaf plkqmqnlat prlaavqwdt llnwqlnkal rvteadrvia tsvkllqavd
      361 yllsslpaev vlhhlawslv qmigwmadsa lpgrptayem edmrsadcfw vthrafgvth
      421 lsgyinttyp lhwrssvdai lmtitqtain fflktswiha esraaavdkl rrmntslwps
      481 dsylnaseva ailenfpepr edgavlrywl dalsarrall grqwdvadaf ypgdlqppra
      541 lfryhyylne lavslhalrs plfvegvgla vnmgglgahy avalarafdp rgvlldgrgs
      601 tsglwwgkss yrdyerrtac pataasgaeg gvqafegvaa ieiaygalna srsiadqgtw
      661 thqrrriflg lsedqaffva fcqascargp serqqlswtl plknvrefat aftcpvaspm
      721 npalrcgffe asdqraarkn ggesedagkr astnasanvg adgavtswtr pparrsgsdd
      781 qdpltlaptg laghmvrqpp vl
//
LOCUS       XP_077508124             767 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 2-like [Amblyomma americanum].
ACCESSION   XP_077508124
VERSION     XP_077508124.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077651998.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..767
                     /product="endothelin-converting enzyme 2-like"
                     /calculated_mol_wt=84060
     Region          93..726
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..767
                     /gene="LOC144119353"
                     /coded_by="XM_077651998.1:1..2304"
                     /db_xref="GeneID:144119353"
ORIGIN      
        1 magegckaps ttsggldrrq ravalghlls sllgvaltls aitiglsvlr hqrgsegsgv
       61 fgmgrsswnt cnsklcmahf rrlkdslnrs tapcgdfyry acgawhpraa ksetvfrdll
      121 avaqqdairy leavndasnk mvlptekvll pdsrpvlrin laerahqvcl hsgsstkdaa
      181 lltefladrs vswperttap aqpldvlldl dinwnlglwf svrvspipgc nqrqvrvgsg
      241 ivsydwkral dtmvtrgvyk arvrrfhdal rarsfaamsd kemkehrrye stivgalcgs
      301 vsggqnelaf plkqmqnlat prlataqwdt llnwqlnkal rvteadrvia tnvkllqavd
      361 yllsslpaev vlhqlawslv qmigwmadsa lpgrptayem edmrsadcfw vthrafgvth
      421 lsgyinttyp lhwrssvdai lmtiiqttin fflktswiha esraaavdkl rrmntslwps
      481 dsylnaseva ailenfpepr edgavlqywl dalsarrall grqwdvadaf ypgdllppra
      541 lfryhyylne lavslhalrs plfvegvgla vnmgglgahy avalarafdp rgvlldgrgs
      601 ssglwwgkss yrdyerrtac pataasgakg gvqafegvaa ieiaygafna srsiadqgtr
      661 thqrrriflg lsedqaffva fcqascargp serqqlswtl plknvrefat aftcpvaspm
      721 npalrcgffe asdqraarkn ggesedagks astnasanvg adgavts
//
LOCUS       XP_077508131             748 aa            linear   INV 25-NOV-2025
DEFINITION  neprilysin-1-like [Amblyomma americanum].
ACCESSION   XP_077508131
VERSION     XP_077508131.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652005.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..748
                     /product="neprilysin-1-like"
                     /calculated_mol_wt=82452
     Region          91..726
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..748
                     /gene="LOC144119362"
                     /coded_by="XM_077652005.1:1..2247"
                     /db_xref="GeneID:144119362"
ORIGIN      
        1 magegceaps ttsggldrrq ravplgplls sflgvaltls aitiglsvlr hrrgsegsgv
       61 fgmgrsswnt cnsklcmahf rrlkdslnrs aapcgdfyrf acgawhpraa ksetvfrdll
      121 avaqqdairy leavsdasnk mvlptgkvll pdsrpvlcin raerayqvcl hsgsstrdaa
      181 llteflanrs lswperttap aqpldvlldl dinwnlglwf svrvspipgr nqrqvrvgsg
      241 ivsydwkral dtmvtrgvyk arvrrfhdal rarsfaamsd keveelrrye stivialsgs
      301 vsggqnelaf plkqmqslat prlataqwvt llnwqlnkal rvteadrvia tnvkllqavg
      361 yllsslsaev vlhqltwslv qmigwmadsa lpgrptayem rdmrsadcfw athrafgvtl
      421 lsgyinttyp lhwrssvdai lmtitktain lflktswida esraaavdkl rrmntslwps
      481 dsylnaseva ailedfpepr edgavlrywl dalsarrall drqwdvadaf ypgdlqppra
      541 lfrchyylne lavslhalrr plfvegvgla vnmgglgahf aaalarafdp rgvlldgrgs
      601 tsglwwgkss yrdyerrtac pataasgaeg gvqafegvaa ieiayqafna srciadqvtr
      661 ihqrrrtflg lsedqaffva fceascargp sertqrscrl plknvrefat aftcpvaspm
      721 npalrcgffd asdqraarkn ggesgrcr
//
LOCUS       XP_077508138             748 aa            linear   INV 25-NOV-2025
DEFINITION  neprilysin-1-like [Amblyomma americanum].
ACCESSION   XP_077508138
VERSION     XP_077508138.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652012.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..748
                     /product="neprilysin-1-like"
                     /calculated_mol_wt=82452
     Region          91..726
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..748
                     /gene="LOC144119372"
                     /coded_by="XM_077652012.1:1..2247"
                     /db_xref="GeneID:144119372"
ORIGIN      
        1 magegceaps ttsggldrrq ravplgplls sflgvaltls aitiglsvlr hrrgsegsgv
       61 fgmgrsswnt cnsklcmahf rrlkdslnrs aapcgdfyrf acgawhpraa ksetvfrdll
      121 avaqqdairy leavsdasnk mvlptgkvll pdsrpvlcin raerayqvcl hsgsstrdaa
      181 llteflanrs lswperttap aqpldvlldl dinwnlglwf svrvspipgr nqrqvrvgsg
      241 ivsydwkral dtmvtrgvyk arvrrfhdal rarsfaamsd keveelrrye stivialsgs
      301 vsggqnelaf plkqmqslat prlataqwvt llnwqlnkal rvteadrvia tnvkllqavg
      361 yllsslsaev vlhqltwslv qmigwmadsa lpgrptayem rdmrsadcfw athrafgvtl
      421 lsgyinttyp lhwrssvdai lmtitktain lflktswida esraaavdkl rrmntslwps
      481 dsylnaseva ailedfpepr edgavlrywl dalsarrall drqwdvadaf ypgdlqppra
      541 lfrchyylne lavslhalrr plfvegvgla vnmgglgahf aaalarafdp rgvlldgrgs
      601 tsglwwgkss yrdyerrtac pataasgaeg gvqafegvaa ieiayqafna srciadqvtr
      661 ihqrrrtflg lsedqaffva fceascargp sertqrscrl plknvrefat aftcpvaspm
      721 npalrcgffd asdqraarkn ggesgrcr
//
LOCUS       XP_077508141             802 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 2-like [Amblyomma americanum].
ACCESSION   XP_077508141
VERSION     XP_077508141.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652015.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..802
                     /product="endothelin-converting enzyme 2-like"
                     /calculated_mol_wt=87768
     Region          93..726
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..802
                     /gene="LOC144119381"
                     /coded_by="XM_077652015.1:1..2409"
                     /db_xref="GeneID:144119381"
ORIGIN      
        1 magegckaps ttsggldrrq ravalghlls sllgvaltls aitiglsvlr hqrgsegsgv
       61 fgmgrsswnt cnsklcmahf rrlkdslnrs tapcgdfyrf acgawhpraa ksetvfrdll
      121 avaqqdairy leavndasnk mvlptgkvll pdsrpvlrin raerahqvcl hsgsstkdaa
      181 lltefladrs lswperttap aqpldvlqdl dinwnlglwf svrvspipgc nqrqvrvgsg
      241 ivsydwkral dtmvtrgvyk arvrrfhdal rarsfaamsd kgmkelrrye stivgalcgs
      301 vsggqnelaf plkqmqnlat prlaaaqwdt llnwqlnkal rvteadrvia tsvkllqavd
      361 yllsslpaev vlhqlawslv qmigwmadsa lpgrptayem edmrsadcfw vthrafgvth
      421 lsgyinttyp lhwlssvdai lmtitqtaik fflktswiha esraaavdkl rrmntslwps
      481 dsylnaseva ailenfpepr edgavlrywl dalsarrall grqwdvadaf ypgdlqppra
      541 lfryhyyrne lavslhalrs plfvegvgla vnmgglgahy avalarafdp rgvlldgrgs
      601 tsglwwgkss yrdyerrtac pataasgaeg gvqafegvaa ieiaygafna srsiadqgtr
      661 thqrrriflg lsedqaffva fceascargp serqqlsctl plknfrefat aftcpvaspm
      721 npalrccffe asdqraarkn ggesedagks astnasanvg adgavtswtr pparrsgsdd
      781 qdpltlatrg laghmvrqpp vl
//
LOCUS       XP_077508146             802 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 2-like [Amblyomma americanum].
ACCESSION   XP_077508146
VERSION     XP_077508146.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652020.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..802
                     /product="endothelin-converting enzyme 2-like"
                     /calculated_mol_wt=87576
     Region          93..726
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..802
                     /gene="LOC144119393"
                     /coded_by="XM_077652020.1:1..2409"
                     /db_xref="GeneID:144119393"
ORIGIN      
        1 magegckaps ttsggldrrq ravalghlls sllgvaltls aitiglsvlr hqrgsegsgv
       61 fgmgrsswnt cnsklcmahf rrlkdslnrs tapcgdfyrf acgawhpraa ksetvfrdll
      121 avaqqdairy leavidasnk mvlptgkvll pdsrpvlrin raerahqvcl hsgsstkdaa
      181 lltefladrs lswperttap aqpldvlldl dmnwnlglwf svcvspipgc nqrqvrvgsg
      241 ivsynwkral dtmvtrgvyk arvrrfhdal rarsfaamsd kemkelrrye stivgalcgs
      301 vsggqnelaf plkqmqnlat prlaaaqwdt llnwqlnkal rvteadrvia tsvkllqavd
      361 yllsslpaev vlhqlawslv qmigwmadsa lpgrptayem edmrsadcfw vthrafgvth
      421 lsgyinttyp lhwlssvdai lmtitqtain fflktswiha esraaavdkl rrmntslwps
      481 dsylnaseva ailenfpepr edgavlrywl dalsarrall grqwdvadaf ypgdlqppra
      541 lfryhyyrne lavslhalrs plfvegvgla vnmgglgahy avalarafdp rgvlldgrgs
      601 tsglwwgkss yrdyerrtac pataasgaeg gvqafegvaa ieiaygafna srsiadqgtr
      661 thqrrriflg lsedqaffva fceascargp serqqlsctl plknfrefat aftcpvaspm
      721 npalrcgffe asdqraarkn ggesedagks astnasanvg adgavtsctr pparrsgsdd
      781 qdpltlatsg laghmvrqpp vl
//
LOCUS       XP_077508154             802 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 2-like [Amblyomma americanum].
ACCESSION   XP_077508154
VERSION     XP_077508154.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652028.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..802
                     /product="endothelin-converting enzyme 2-like"
                     /calculated_mol_wt=87760
     Region          93..726
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..802
                     /gene="LOC144119401"
                     /coded_by="XM_077652028.1:1..2409"
                     /db_xref="GeneID:144119401"
ORIGIN      
        1 magegckaps ttsggldrrq ravalghlls sllgvaltls aitiglsvlr hqrgsegsgv
       61 fgmgrsswnt cnsklcmahf rrlkdslnrs tapcgdfyrf acgawhprta ksdsvfrdll
      121 avaqqdairy leavndasnk mvlptgkvll pdsrpvlrin raerahqvcl hsgsstkdaa
      181 lltefladrs lswperttap aqpldvlldl dinwnlglwf svrvspipgc nqrqvrvgsg
      241 ivsydwkral dtmvtrgvyk arlrrfhdal rarsfaamsd kemkelrrye ssivgalcgs
      301 vsggqnelaf plkqmqnlat prlaavqwdt llnwqlnkal rvteadrvia tsvkllqavd
      361 yllsslpaev vlhhlawslv qmigwmadsa lpgrptayem edmrsadcfw vthrafgvth
      421 lsgyinttyp lhwrssvdai lmiitqtain fflktswiha esraaavdkl rrmntslwps
      481 dsylnaseva ailekfpepr edgavlrywl dalsarrall grqwdvadaf ypgdlqppra
      541 lfryhyylne lavslhalrs plfvegvgla vnmgglgahy avalarafdp rgvlldgrgs
      601 tsglwwgkss yrdyerrtac pataasgaeg gvqafegvaa ieiaygafna srsiadqgtr
      661 thqrrriflg lsedqgffva fcqascargp serqqlsctl plknfrefat aftcpvaspm
      721 npalrcgffe asdqraarkn ggesedagks astnasanvg adgavtswtr pparrsgsdd
      781 qdpltlattg laghmvrqpp vl
//
LOCUS       XP_077508161             802 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 2-like [Amblyomma americanum].
ACCESSION   XP_077508161
VERSION     XP_077508161.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652035.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..802
                     /product="endothelin-converting enzyme 2-like"
                     /calculated_mol_wt=87879
     Region          93..726
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..802
                     /gene="LOC144119411"
                     /coded_by="XM_077652035.1:1..2409"
                     /db_xref="GeneID:144119411"
ORIGIN      
        1 magegckaps ttsggldrrq rvvalghlls sllgvaltls aitiglsvlr hqrgsegsgv
       61 fgmgrsswnt cnsklcmahf rrlkdslnrs tapcgdlyrf acgawhpraa ksetvfrdll
      121 avaqqdairy leavndasnk mvlptgkvll pdsrpvlrin raerahqvcl hsgsstkdaa
      181 lltefladrs lswperttap aqpldvlldl dinwnlglwf svrvspipgc nqrqvrvgsg
      241 ivsydwkral dtmvtrgvyk arvrrfhdal rarsfaamsd kemkelrrye stivgalcgs
      301 vsggqnelaf plkqmqnlat prlaaaqwdt llnwqlnkal rvteadrvia tsvkllqavd
      361 yllsslpaev vlhhlawslv qmigwmadsa lpgrptayem edmrsadcfw vthrafgvth
      421 lsgyinttyp lhwrssvdai lmtitqtain fflktswiha esraaavdkl rrmntslwps
      481 dsylnaseva ailenfpepr edgavlrywl dalsarralf grqwdvadaf ypgdlqppra
      541 lfryhyylne lavslhalrs plfvegvgla vnmgglgahy avalarafdp rgvlldgrgs
      601 tsglwwrkss yrdyerrtac pataasgaeg gvqafegvaa ieiaygafna srsiadqgtr
      661 thqrrriflg lsedqgffva fcqascargp serqqlsctl plknfrefat aftcpvaspm
      721 npalrcgffe atdqraarkn ggesedagks astnasanvg adgavtswtr pparrsgsdd
      781 qdpftlattg laghmvrqpp vl
//
LOCUS       XP_077508169             802 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 2-like [Amblyomma americanum].
ACCESSION   XP_077508169
VERSION     XP_077508169.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652043.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..802
                     /product="endothelin-converting enzyme 2-like"
                     /calculated_mol_wt=87781
     Region          93..726
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..802
                     /gene="LOC144119418"
                     /coded_by="XM_077652043.1:1..2409"
                     /db_xref="GeneID:144119418"
ORIGIN      
        1 magegckaps ttsggldrrq ravalghlls sllgvaltls aitiglsvlr hqrgsegsgv
       61 fgmgrsswnt cnsklcmahf rrlkdslnrs tapcgdfyrf acgawhpraa ksetvfrdll
      121 avaqqdairy leavndasnk mvlptgkvll pdsrpvlrin raerahqvcl hsgsstkdaa
      181 lltefladrs lswperttap aqpldvlqdl dinwnlglwf svrvspipgc nqrqvrvgsg
      241 ivsydwkral dtmvtrgvyk arvrrfhdal rarsfaamsd kemkelrrye stivgalcgs
      301 vsggqnelaf plkqmqnlat prlaaaqwdt llnwqlnkal rvteadrvia tsvkllqavd
      361 yllsslpaev vlhqlawslv qmigwmadsa lpgrptayem edmrsadcfw vthrafgvth
      421 lsgyinttyp lhwhssvdai lmtitqtain fflktswiha esraaavdkl rrmntslwps
      481 dsylnaseva ailenfpepr edgavlrywl dalsarrall grqwdvadaf ypgdlqppra
      541 lfryhyylne lavslhalrc plfvegvgla vnmgglgahy avalarafdp rgvlldgrgs
      601 tsglwwgkss yrdyerrtac pataasgaeg gvqafegvaa ieiaygafna srsiadqgtr
      661 thqrrriflg lsedqgffva fcqsscargp serqqlsctl plknfrefst aftcpvaspm
      721 npalrcgffe asdqraarkn ggesedagks astnaisnvg adgavtswtr pparrsgsdd
      781 qdpltlattg laghmvrqpp vl
//
LOCUS       XP_077508173             740 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme-like 1 [Amblyomma americanum].
ACCESSION   XP_077508173
VERSION     XP_077508173.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652047.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..740
                     /product="endothelin-converting enzyme-like 1"
                     /calculated_mol_wt=81975
     Region          114..735
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..740
                     /gene="LOC144119429"
                     /coded_by="XM_077652047.1:1..2223"
                     /db_xref="GeneID:144119429"
ORIGIN      
        1 mngqlavprr hewrpqrmws rmagegceap sttsggvdrr qravalghll ssllgvaltl
       61 saitiglsvl rhqrgsegsg vfgmgrsswn tcnsklcmah frrlkdslnr stapcgdfyr
      121 facgawhpra aksetafrdl lavaqqdair yleavndasn kmvlptgkvl vpdsrpvlri
      181 nraeracqvc lhsgsstrda allteflanr slswpertta pappldvlld ldinwnlglw
      241 fwvrvspipg rnqrqvrvgs givssdwkra ldtmvtrgvy karvrrfhda lrarsfaams
      301 dkemkelrry estivgalcg svsggqnela fplkqmqnla tprlaaaqwd tllnwqlnka
      361 lrvteadrvi atsvkllqav dyllsslpae vvlhhlawsl vqmigwmads alpgrptaye
      421 medmrsadcf wvthrafgvt hlsgyintty plhwhssvda ilmtitqtai nfflktswih
      481 aesraaavdk lrrmntslwp sdsylnasev aailenfpep redgavlryw ldalsarral
      541 lgrqwdvada fypgdlqppr alfryhyyln elavslhalr splfvegvgl avnmgglgah
      601 yavvlarafd prgvlldgrg stsglwwgks syrdyerrta cpataasgae ggvqafegva
      661 aieiaygafn asrsiadqgt rthqrrrifl glsedqgffv afcqsscarg pserqqlsct
      721 lplknfpssp phlrvpwprp
//
LOCUS       XP_077508175             802 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 2-like [Amblyomma americanum].
ACCESSION   XP_077508175
VERSION     XP_077508175.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652049.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..802
                     /product="endothelin-converting enzyme 2-like"
                     /calculated_mol_wt=87632
     Region          93..726
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..802
                     /gene="LOC144119440"
                     /coded_by="XM_077652049.1:1..2409"
                     /db_xref="GeneID:144119440"
ORIGIN      
        1 magegceaps ttsggldrrq ravalghlls sllgvaltls aitiglsllr hqrgsegsgv
       61 fgmgrsswnt cnsklcmahf rrlkdslnrs tapcgdfyrf acgawhpraa ksetafrdll
      121 avaqqdairy leavndasnk mvlptgkvlv pdsrpvlrin raeracqvcl hsgsstrdaa
      181 llteflanrs lswperttap aqpldvlldl ginwnlglwf wvrvspipgr nqrqvrvgsg
      241 ivssdwkral dtmvtrgvyk arvrrfhdal rarsfaaksd keveelrrye stivgalsgs
      301 vsggqnelaf plkqmqnlat prlataqwvt llnwqfnkal rvteadrvia tnvkllqavd
      361 yllsslpaev vlhqlawslv qmlgwmadsa lpgrptayem gdmrsadcfw athrafgvtl
      421 lsgyinttyp lhwrssvdal lmtitptain lflktswida esraaavdkl rrmntflwps
      481 dsylnaseva ailenfpelr edgavlrywl dalsarrall grqwdvadaf ypgdlqppra
      541 lfryhyylne lavslhalrs plfvegvgla vnmgglgahy aaalarafdp rgvlldgrgs
      601 tsglwwgkss yrdyerrtac pataasgadg gvqafvgvaa teiayqafna srsiadqgtr
      661 thqrrriflg lsedqaffva fcqascargs serqqlyctl plknfrefat aftcpvaspm
      721 npalrcgffe asdqraarkn ggesedagks astnasanvg adgavtswtr pparrsgsdd
      781 qdpltlaptg laghmvrqpp vl
//
LOCUS       XP_077508183             802 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 2-like [Amblyomma americanum].
ACCESSION   XP_077508183
VERSION     XP_077508183.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652057.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..802
                     /product="endothelin-converting enzyme 2-like"
                     /calculated_mol_wt=87611
     Region          93..726
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..802
                     /gene="LOC144119450"
                     /coded_by="XM_077652057.1:1..2409"
                     /db_xref="GeneID:144119450"
ORIGIN      
        1 magegceaps ttsggldrrq ravalghlls sllgvaltls aitiglsllr hqrgsegsgv
       61 fgmgrsswnt cnsklcmahf rrlkdslnrs tapcgdfyrf acgawhpraa ksetafrdll
      121 avaqqdairy leavndasnk mvlptgkvlv pdsrpvlrin raeracqvcl hsgsstrdaa
      181 llteflanrs lswperttap aqpldvlldl dinwnlglws wvrvspipgr nqrqvrvgsg
      241 ivssdwkral dtmvtrgvyk arvrrfhdal rarsfaaksd keveelrrye stivgalsgs
      301 vsggqnelaf plkqmqnlat prlataqwvt llnwqfnkal rvteadrvia tnvkllqavd
      361 yllsslpaev vlhqlawslv qmlgwmadsa lpgrptayem gdmrsadcfw athrafgvtl
      421 lsgyinttyp lhwrssvdal lmtitptain lflktswida esrgaavdkl rrmntslwps
      481 dsylnaseva ailenfpelr edgavlrywl dalsarrall grqwdvadaf ypgdlqppra
      541 lfryhyylne lavslhalrs plfvegvgla vnmgglgahy aaalarafdp rgvlldgrgs
      601 tsglwwgkss yrdyerrrac pataasgadg gvqafvgvaa teiayqafna srsiadqgtr
      661 thqrrriflg lsedqaffva fcqascargs serqqlyctl plknfrefat aftcpvaspm
      721 npalrcgffe asdqraarkn ggesedagks astnasanvg adgavtswtr pparrsgsdd
      781 qdpltlaptg laghmvrqpp vl
//
LOCUS       XP_077508195             274 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119463 [Amblyomma americanum].
ACCESSION   XP_077508195
VERSION     XP_077508195.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652069.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..274
                     /product="uncharacterized protein LOC144119463"
                     /calculated_mol_wt=30460
     CDS             1..274
                     /gene="LOC144119463"
                     /coded_by="XM_077652069.1:1..825"
                     /db_xref="GeneID:144119463"
ORIGIN      
        1 mgrtrstssv ivqvastdda cstsnqpain emlaellagq rkmaediadi krfqqivnsr
       61 fealesrvsa lesssptsng dasdgavqhq lnslsevvsk lasrndelen rsrrnnlifh
      121 glaeedeetn kalfiavtkv fidnlqldcp kieryhrlgr kqegrprpvi vklldfrekt
      181 qalknahrlk nsgislsedf sahvrlirkk iwdatqsypd sgskvhlryd hvfidsvryn
      241 wddvekslvk dsksmppdta aanataakpp adsd
//
LOCUS       XP_077508200             241 aa            linear   INV 25-NOV-2025
DEFINITION  YEATS domain-containing protein 4-like [Amblyomma americanum].
ACCESSION   XP_077508200
VERSION     XP_077508200.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652074.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..241
                     /product="YEATS domain-containing protein 4-like"
                     /calculated_mol_wt=27509
     Region          23..159
                     /region_name="YEATS_GAS41_like"
                     /note="YEATS domain found in YEATS domain-containing
                     protein 4 and similar proteins; cd16909"
                     /db_xref="CDD:341128"
     CDS             1..241
                     /gene="LOC144119472"
                     /coded_by="XM_077652074.1:62..787"
                     /db_xref="GeneID:144119472"
ORIGIN      
        1 mavvmpgiis eivqepggrm kgltivkpiv ygnvaryfgk kredghthqw tvylkpyrne
       61 dmssyvqkvh fklhesypnq nrlmvtkppy evtetgwgef eivikiyfmd tterpvtvyh
      121 ilklfqsetn imlvkkqlvs esydelifse ptvimhqhlt ntrplsvgpy khefdfkewk
      181 ektldsilsa knkirlevad lkerlkvare tigkykaeva klesqddadt gggavapsaa
      241 s
//
LOCUS       XP_077508211             276 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119489 [Amblyomma americanum].
ACCESSION   XP_077508211
VERSION     XP_077508211.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652085.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 26% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..276
                     /product="uncharacterized protein LOC144119489"
                     /calculated_mol_wt=30616
     Region          <89..125
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..276
                     /gene="LOC144119489"
                     /coded_by="XM_077652085.1:1..831"
                     /db_xref="GeneID:144119489"
ORIGIN      
        1 mrlvtglpqf cpvedlhacs kinalqdvae qhlqaqrvrl sttaagrqil ralgyntdkl
       61 ellsspappw elidlveayt acraggadya vlsairtear rlreegytiv irwipahegi
      121 tgnekahqaa raaalsaplp gctlvsaswa rddvahpssp pmdraetwhr aqcyrrrilq
      181 hvanpeqlyr rpprtfrrve avllrraqtg qllspaklnl inphsyptpq cpacpargta
      241 ehllwrcpaf dvvreralqs lgghrpgtlh ewavel
//
LOCUS       XP_077508226             536 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119507 [Amblyomma americanum].
ACCESSION   XP_077508226
VERSION     XP_077508226.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652100.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..536
                     /product="uncharacterized protein LOC144119507"
                     /calculated_mol_wt=58315
     Region          261..388
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     Site            order(265..268,297..299,302,327,366,384)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260008"
     CDS             1..536
                     /gene="LOC144119507"
                     /coded_by="XM_077652100.1:1..1611"
                     /db_xref="GeneID:144119507"
ORIGIN      
        1 mlfsprrlaa ntmpplqlqg tplpwqsrgk ylglhinakl nykaaivspa kteallyhpr
       61 grrarittlr lgadgltwrd qvkylgllid rrltwlpavr allprlrrig qvvqrlqard
      121 kgcspswair lfhaaatslv tyalplvtlp parlrtlelg hrsvlrrvca mstayimlqt
      181 egpfclgsae hrtpawgscv ahstgssapl strhhsrpqt kmpplpisvd irscsrrrtp
      241 vaalqqtaat mlqeslgghl qvytdgsvlp atgqaaaacv apalgaalsc rlrfpansta
      301 aelaglhlaa dlllllpgqq paviltdsra alqllrqhqp rqhtvanlta rlmaiqdagr
      361 pvslqwlpsh vgitgneaad qlagaahtng spvsskvtql dfahpalqqa vralhpdprv
      421 asgvrfmrlp dclprrertl llrlrtgcsw tqarlhrhar apsqacsfcg adetlghllc
      481 acpnleaarr ditagyrnlg lpsysdqdll hpersqteaf rllleflqst glatrl
//
LOCUS       XP_077508241             173 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119527 [Amblyomma americanum].
ACCESSION   XP_077508241
VERSION     XP_077508241.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652115.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..173
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..173
                     /product="uncharacterized protein LOC144119527"
                     /calculated_mol_wt=18920
     Region          <57..>92
                     /region_name="PRK09752"
                     /note="AIDA-I family autotransporter YfaL"
                     /db_xref="CDD:182059"
     CDS             1..173
                     /gene="LOC144119527"
                     /coded_by="XM_077652115.1:1..522"
                     /db_xref="GeneID:144119527"
ORIGIN      
        1 mkqnkdihar itiievsegh gppernlhat sgpkidednl rgdergasas crqdtlsptd
       61 ptpvpsphps qqhtpepplq psscatpsll gkrrrddgan evlhqtlyes hhldsltear
      121 nrqdaafqqs mleairevad avqasngqqe llirnvnlpt lilqkqlepp aph
//
LOCUS       XP_077508259             140 aa            linear   INV 25-NOV-2025
DEFINITION  putative nicotine oxidoreductase [Amblyomma americanum].
ACCESSION   XP_077508259
VERSION     XP_077508259.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652133.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..140
                     /product="putative nicotine oxidoreductase"
                     /calculated_mol_wt=15175
     Region          2..>132
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(36..41,95..96,126,128..129)
                     /site_type="active"
                     /db_xref="CDD:238185"
     Site            order(36..41,95,128)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238185"
     Site            96
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     CDS             1..140
                     /gene="LOC144119548"
                     /coded_by="XM_077652133.1:1..423"
                     /db_xref="GeneID:144119548"
ORIGIN      
        1 mfgfraklst qdvllqikev ltqvpyngen vimaldvkgt cdnvghavid glnnanfgkk
       61 ihdytrafls nrtamvgige lrgekintpn kgtpqgsvis pvlfnivmie larrlgkied
      121 igfaiyaddi kiwanqgtlg
//
LOCUS       XP_077508269             289 aa            linear   INV 25-NOV-2025
DEFINITION  sodium-coupled monocarboxylate transporter 1-like [Amblyomma
            americanum].
ACCESSION   XP_077508269
VERSION     XP_077508269.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652143.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 22% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..289
                     /product="sodium-coupled monocarboxylate transporter
                     1-like"
                     /calculated_mol_wt=30925
     Region          <3..227
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     CDS             1..289
                     /gene="LOC144119559"
                     /coded_by="XM_077652143.1:1..870"
                     /db_xref="GeneID:144119559"
ORIGIN      
        1 mamsiiywyr dcdprlagai prydqivpyy inenlsilpg mrgvflagvv gasmstvssi
       61 inshsavfyv dvvtpyfska kkrpsyvisi iaiasgvmmt gfatllpymg taarvtmvic
      121 nsisgplcgl fllallfpws ntkgaasstl vslalllwhs vgrsfsgiep prmdvsidgc
      181 lanvtavsts alrhvrldvf pfyrisanwm svfsvlftia tglvvslatg grrevdkslq
      241 lsskqvlcvw aqvgllsrkt lvsdgspems tskeaislip rsgeeagac
//
LOCUS       XP_077508279             169 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119570 [Amblyomma americanum].
ACCESSION   XP_077508279
VERSION     XP_077508279.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652153.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..169
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..169
                     /product="uncharacterized protein LOC144119570"
                     /calculated_mol_wt=18507
     CDS             1..169
                     /gene="LOC144119570"
                     /coded_by="XM_077652153.1:1..510"
                     /db_xref="GeneID:144119570"
ORIGIN      
        1 mvatrniqga idgpfsllmc itmmrwpfpg sllqamvvli laqaaharcs vltptlrllp
       61 lhishpengs swehtaahlq lmvhlngggg pqtadrttef lwqcalpqlr aitkkmhspv
      121 htygevslpe qvqgvlrrgp kfaveprqsa sellametyr eplmglshc
//
LOCUS       XP_077508306             196 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119602 [Amblyomma americanum].
ACCESSION   XP_077508306
VERSION     XP_077508306.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652180.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..196
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..196
                     /product="uncharacterized protein LOC144119602"
                     /calculated_mol_wt=21366
     Region          <80..>115
                     /region_name="PRK09752"
                     /note="AIDA-I family autotransporter YfaL"
                     /db_xref="CDD:182059"
     CDS             1..196
                     /gene="LOC144119602"
                     /coded_by="XM_077652180.1:1..591"
                     /db_xref="GeneID:144119602"
ORIGIN      
        1 maafkktadp tasscfftrr lksgfylvvq saritiievs eghgppernl hatsgpkide
       61 dnlrgderga sascrqdtls ptdptpvpsp hpsqqhtpep plqpsscatp sllgkrrrdd
      121 ganevlhqtl yeshhldslt earnrqdaaf qqsmleaire vadavqasng qqellirnvn
      181 lptlilqkql eppaph
//
LOCUS       XP_077508331             710 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-4-like [Amblyomma americanum].
ACCESSION   XP_077508331
VERSION     XP_077508331.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652205.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..710
                     /product="protein argonaute-4-like"
                     /calculated_mol_wt=81098
     Region          149..208
                     /region_name="ArgoN"
                     /note="N-terminal domain of argonaute; pfam16486"
                     /db_xref="CDD:465134"
     Region          221..335
                     /region_name="PAZ"
                     /note="PAZ domain; pfam02170"
                     /db_xref="CDD:460472"
     Region          362..690
                     /region_name="Piwi-like"
                     /note="PIWI domain. Domain found in proteins involved in
                     RNA silencing. RNA silencing refers to a group of related
                     gene-silencing mechanisms mediated by short RNA molecules,
                     including siRNAs, miRNAs, and heterochromatin-related
                     guide RNAs. The central component...; cl00628"
                     /db_xref="CDD:412485"
     Site            order(494,498,510..513,516,525,528,532,536)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     CDS             1..710
                     /gene="LOC144119632"
                     /coded_by="XM_077652205.1:1..2133"
                     /db_xref="GeneID:144119632"
ORIGIN      
        1 mevvegdevd preftapgqw fqcrgkkvvp leksrreiea eqfrlrqagr klaafseier
       61 tlpshfpcrp aqgklgrpiq ltanyfniei pagnvfyydv eicsetrkea kvpenrkycc
      121 istkinrlvi ellvkkyrvd lngcmpafdg rknlytriep kfrtrtftvd feenqriqkf
      181 ivkiqyagtv nlealhavfd nrvsqvphgv pharagarcr pdfrelrdsq nvrlnkelkg
      241 lrvkvthlpy prkykvirvt kepakklffn medgfccava dyfykryhrl sypnlpciqt
      301 gsathpvylp levcvivegq hckkkldenq tsemikrtaq ppakrfnetr qtvrdlvsss
      361 dqclhefgik isteqtqlrg rvldppslvf ennsigkpre gtwelrgrhf yepamltrwt
      421 llnlsrfaqr dslhnfvrml irvgqelgmc ieqpldvttt danrkpvrni lleeqrktvs
      481 lemviimlak ntnyaeikqv aeteiglrtq cvmdnnvikk csaaliinlc qkinakldgi
      541 nsllpkekpk ifqkpviiig advthpapgd klrlsiaacv gsldsipskf hasiriqmed
      601 saatspwrss risrvsairl acqelspnet yepaltftvv qkrhhtrfmp tndrdgvgqc
      661 rnvppgttvd svvthpldfd fylcshfgnq vhsngmpsga psvnsrywwl
//
LOCUS       XP_077508339             464 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119642 [Amblyomma americanum].
ACCESSION   XP_077508339
VERSION     XP_077508339.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652213.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..464
                     /product="uncharacterized protein LOC144119642"
                     /calculated_mol_wt=47725
     Region          <45..258
                     /region_name="KAR9"
                     /note="Yeast cortical protein KAR9; pfam08580"
                     /db_xref="CDD:430088"
     Region          <183..422
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..464
                     /gene="LOC144119642"
                     /coded_by="XM_077652213.1:183..1577"
                     /db_xref="GeneID:144119642"
ORIGIN      
        1 mskdgatrgr tnghkqerlt qrshllprvp tagnhgpsgr sqaapsalpl ngtaqrsfaq
       61 vvqervvtaa ppqlrsrtan vtthgegtts ngartvaptk gsptnlplpm aapppllrsr
      121 tanvttrgeg ttshgartva ptkgrptnlp sptaapppll rsrtanvttr gegttshgsr
      181 tvaptkgspt nlpsptaapp pklrsrrtan vttrgegtis hgartvaptk gsptnlpspt
      241 aapppqlhsr rtanvttrge gttsnrartv aptkgsptnl lsptaapppk rrsrrtanvt
      301 trgegttsnr artvaptkgs ptnllsptaa pppkrrsrrt anvttrgegt tsngartvap
      361 tkgsptnmps ptaapppqrr srrtanvttr gegttsnrar tvaptkgspt nlpaptaapv
      421 eavqarngvg naklqndelk iqvsggcccl pprtclnsvd gcgr
//
LOCUS       XP_077508349             902 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077508349
VERSION     XP_077508349.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652223.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..902
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=103193
     Region          138..207
                     /region_name="ArgoN"
                     /note="N-terminal domain of argonaute; pfam16486"
                     /db_xref="CDD:465134"
     Region          217..268
                     /region_name="ArgoL1"
                     /note="Argonaute linker 1 domain; pfam08699"
                     /db_xref="CDD:462567"
     Region          303..404
                     /region_name="PAZ"
                     /note="PAZ domain; pfam02170"
                     /db_xref="CDD:460472"
     Region          433..859
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(563,567,579..582,585,594,597,601,605)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(632,634,710,850)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..902
                     /gene="LOC144119653"
                     /coded_by="XM_077652223.1:1..2709"
                     /db_xref="GeneID:144119653"
ORIGIN      
        1 mafptveqst vefsnfqnlv qecwtrvvrc gnlnlsrgls rrglqeiera lpshfprrpa
       61 qgtlgrsihl ianyfsveip sgnffhydve icsktqkkak vpekrkyrci stkinrlvme
      121 llvkkyrvdl ngcmpaydgr knlytrrelk frertftvdf eedqriqkfi vkiqyaatvn
      181 ldvlhavfdn risqvpqevl qavdivmrhg psikltpvgr yffqapsykd yytlgggrev
      241 wfgyyasvrp aqwkpmlnid msatafyqpl pvtnfmckll sedccemsvk dfrelhdsqi
      301 vrlnkelkgl rikvthlpyp rkykvvcvtk esakklffnm edgsrcsvad yfqnryrrls
      361 ypnlpciqtg sttrpvylpl evcvivegqh skkkldenqt semikrtaqp pamrfnqihr
      421 tvrdvvscse qrlhefeini steatqlrgr vldppslvfe nnsigkpreg twelrgrhfy
      481 kpatltrwtl lnlsrfveqd sldnfvkmli rvgqelgmri eqpldvtttd asrklvrnil
      541 leeqrktasl emviivltkn tnyaeikqva eteiglrtqc vmdnnvikkc naalitnlcq
      601 kinakldgin nsllpkempk mfekpviiig advthpapee klrpsiaacv gsldaipskf
      661 hasiriqmed saatsrleii mdlkgmmkem lvafyratrh kpekiiffrd gvsegqfmev
      721 rnrevsairl acqelspnet yepaltfivv qkrhhtrfvp dndrdgvgkc gnvppgttvd
      781 svvthpldfd fylcshlglq gtsrpshyyi vwddsshtad elqklcyylc htyarcsrsv
      841 sipapvyyah laayrarnhv mskvdvssls sdssgggans ittqyveavn vrdslktamy
      901 fv
//
LOCUS       XP_077508361             227 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119664 [Amblyomma americanum].
ACCESSION   XP_077508361
VERSION     XP_077508361.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652235.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..227
                     /product="uncharacterized protein LOC144119664"
                     /calculated_mol_wt=25001
     Region          59..>122
                     /region_name="Arginase_HDAC"
                     /note="Arginase-like and histone-like hydrolases; cl17011"
                     /db_xref="CDD:450134"
     Region          <154..220
                     /region_name="Arginase_HDAC"
                     /note="Arginase-like and histone-like hydrolases; cl17011"
                     /db_xref="CDD:450134"
     CDS             1..227
                     /gene="LOC144119664"
                     /coded_by="XM_077652235.1:1..684"
                     /db_xref="GeneID:144119664"
ORIGIN      
        1 mcgsiystlf nndgsddqts qanvsrskls elpssvsavp nvgyehcasg tstksrvgyv
       61 hspghlfece rlprirkrcf lvhgllkgyn llknlhpihp flasredlcl fhsadyvdfl
      121 esceaaedld gedicvlviq swvlplrllg gggyeaanca rcwtavtaal lgqeldddip
      181 ehrflmaygp gyelsmepgf rrdlndaqyv dgilqevmkh vslfgrs
//
LOCUS       XP_077508371             236 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119675 [Amblyomma americanum].
ACCESSION   XP_077508371
VERSION     XP_077508371.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652245.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..236
                     /product="uncharacterized protein LOC144119675"
                     /calculated_mol_wt=26903
     CDS             1..236
                     /gene="LOC144119675"
                     /coded_by="XM_077652245.1:1..711"
                     /db_xref="GeneID:144119675"
ORIGIN      
        1 mdvftaevkt eitamrtsnn eihselegig eamgfmnesf eafkrdvgkf rrevaevkfq
       61 ntrrqqeipq mqkefvearr eiielkqygr sqnidikgip vvkeddlraa lgkiaasvgv
      121 dvsddaidvv hrvrskdngt pnvvvrfssr avrdkffsaa kktrldtgil gfekseplyi
      181 nehfcvektf lskarklrle kkwkfvwvsq gkilmrktet stvlriesen dlagve
//
LOCUS       XP_077508411             395 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119736 [Amblyomma americanum].
ACCESSION   XP_077508411
VERSION     XP_077508411.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652285.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..395
                     /product="uncharacterized protein LOC144119736"
                     /calculated_mol_wt=43513
     Region          160..>243
                     /region_name="COG4372"
                     /note="Uncharacterized protein, contains DUF3084 domain
                     [Function unknown]"
                     /db_xref="CDD:443500"
     CDS             1..395
                     /gene="LOC144119736"
                     /coded_by="XM_077652285.1:17..1204"
                     /db_xref="GeneID:144119736"
ORIGIN      
        1 mqvfafvana llpallalpp astsstavyc sddrifawpq pdvitsscpa apppgyhfpg
       61 pswkasspat satgyissdr ppsplcglgg casvhttnpf vyimqvsyph slyakrssny
      121 flvqlpspqc ciflvlecah vvrcllilag dietnpgpnn leavfaelkk lsagqtqlis
      181 evqglknqli stdgaiagls krmtdlethl eamtaiksel eiiksentrl srqietlear
      241 iddsenrsrr nnlifygipd snpsetfads egevirhcaq hlsltihpee verahrlgrh
      301 kpnrsrpiiv kfisyktkdt ilsngrkfkg tkfsvgedfs hrvrsvrkql vtfakansdk
      361 falryktlhi gprryifddi sqsvkeitia tpqsd
//
LOCUS       XP_077508426             356 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119753 [Amblyomma americanum].
ACCESSION   XP_077508426
VERSION     XP_077508426.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652300.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..356
                     /product="uncharacterized protein LOC144119753"
                     /calculated_mol_wt=40332
     Region          <4..34
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          256..351
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..356
                     /gene="LOC144119753"
                     /coded_by="XM_077652300.1:1..1071"
                     /db_xref="GeneID:144119753"
ORIGIN      
        1 mtelresfwi psarqqlkki iascpvccrq rlkaatapma plpcdrvsgn qafevvgidf
       61 agplytrrkd gssksyialf tcattraihl elvsnlttec fllalrrfva rrglpstiys
      121 dnaltfkkas kdlqmlwnil rscetqdyit sqqlswkfiv ekaawwggww ermvktvket
      181 lrkilrhqsl sfeelatvla dveatinsrp layasadpre ssvltpshli tgkrlialpe
      241 artasipsat atdvgrrwky rqtiinsfwk rwlkeyilql rsahfasssp asviavddlv
      301 ivhkenaprh mwqtarvvdv fkgrdghirt ctvkmpsgvi irrpvqllyp letnik
//
LOCUS       XP_077508435             483 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119764 [Amblyomma americanum].
ACCESSION   XP_077508435
VERSION     XP_077508435.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652309.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..483
                     /product="uncharacterized protein LOC144119764"
                     /calculated_mol_wt=54273
     Region          8..210
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(73..78,140,142..143,206..207)
                     /site_type="active"
                     /db_xref="CDD:238822"
     Site            order(73..78,142)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238822"
     Region          228..387
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     CDS             1..483
                     /gene="LOC144119764"
                     /coded_by="XM_077652309.1:1..1452"
                     /db_xref="GeneID:144119764"
ORIGIN      
        1 mgakttnvyy mphqpvfrse stttrlrvvf dasshapdkv slndhlekgp kligdlvril
       61 vrfrlheval vadiekaflq igvrpedrda lrflwyrstp vaqeplpsve ewimstllhh
      121 fdkqkgalke tagilkdsfy vddlvtgaks ehealkiate aagivrnagm rlrkwmsnsi
      181 tvrkalrned cgaseetqkv lglhwtpttd rmgislagvl ntistentkr gvlqasasvf
      241 dplglvspfv irakilfqrm wergiewdeq lpsdlkaewt swcsdlsslr qlcfprctap
      301 ndqlswqlhi fcdasptayg ccaylriged sgtvivnlil aksrvaplka lslprlelma
      361 avigtrmaty rrgaidhqln vsfltdsmit lhwirstakn wkpfvqnrvt eiqsasnpes
      421 wrhypgshnp adcltrgmka eslcnskiww hgpewleksa sswpktqsal pcspevvaee
      481 rkr
//
LOCUS       XP_077508445             349 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119775 [Amblyomma americanum].
ACCESSION   XP_077508445
VERSION     XP_077508445.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652319.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..349
                     /product="uncharacterized protein LOC144119775"
                     /calculated_mol_wt=38513
     Region          72..204
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     CDS             1..349
                     /gene="LOC144119775"
                     /coded_by="XM_077652319.1:1..1050"
                     /db_xref="GeneID:144119775"
ORIGIN      
        1 medhlpeeaf etefteaiey ddramntigl lkariaasqp tpavhsaesm qaslptprhs
       61 giklpklhle sfngelanwq tfwecfretv henlslskae kfhylksllt gpassavrgl
      121 qateasydda vqilqrrfgd kgrieqehlt klrmlpsvss sgdvrglrrl ydhvqahicg
      181 laglgvttha ysamlcdivl talprdmvve fhrltgratk tdalssagss paspaqqsas
      241 thtedrsdgr lneilefllv etecrerckq dpvtvaekkp yhidrfgkrp nqhtasaavl
      301 stqpekaekc afcdsskhgt efctaaltkk revrqgkkvl svhevgahi
//
LOCUS       XP_077508471             225 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119805, partial [Amblyomma
            americanum].
ACCESSION   XP_077508471
VERSION     XP_077508471.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652345.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         <1..225
                     /product="uncharacterized protein LOC144119805"
     CDS             1..225
                     /gene="LOC144119805"
                     /coded_by="XM_077652345.1:<1..678"
                     /db_xref="GeneID:144119805"
ORIGIN      
        1 weeeqavlla eaqqkaatla gdgradsprf sakfgtytll dveqnkilhf evvqsnetsg
       61 scqmerrgle laltyleshg ikfegmvtdr hsqiksffek ersdtkhefd vwhvakgvyr
      121 klqtaakkag qeeleprnqs innhlywsaa ssnghkeliv pkwtsllnhi rnvhtheeql
      181 fpvclhgdah piqwlqsgpd lqhfiimrta kedkhaqqtv wlggm
//
LOCUS       XP_077508475             291 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119815 [Amblyomma americanum].
ACCESSION   XP_077508475
VERSION     XP_077508475.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652349.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 30% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..291
                     /product="uncharacterized protein LOC144119815"
                     /calculated_mol_wt=30688
     CDS             1..291
                     /gene="LOC144119815"
                     /coded_by="XM_077652349.1:135..1010"
                     /db_xref="GeneID:144119815"
ORIGIN      
        1 mfagaglneh eilalleavr dvlrpqnpvl lglsggidls egttgdqqvl scdaavqgps
       61 gqqttntdtd steyytnymt yvpdavneht fyaaaevpae dlgtvvtaay vlgsplsfns
      121 tqsdaedgqt ataageeast hnghqlqdqp tvneaapiss ssheqqhttp vsstrrpsaa
      181 aagqtaalpr stprqsrsmq qaghleeviq lnrenvelet aslqaqkraa etvqattqta
      241 adavtkmvha akhflklnda avktiekfgc ladalteltq vqtelpsvhh d
//
LOCUS       XP_077508491             151 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119834 [Amblyomma americanum].
ACCESSION   XP_077508491
VERSION     XP_077508491.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652365.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..151
                     /product="uncharacterized protein LOC144119834"
                     /calculated_mol_wt=16800
     CDS             1..151
                     /gene="LOC144119834"
                     /coded_by="XM_077652365.1:1..456"
                     /db_xref="GeneID:144119834"
ORIGIN      
        1 mlitdaraga smqqhsayst ddstqvtsqk ekkaqkrkls tkscalqlks cssdeeliek
       61 galdakpkei eklqfeirsl rkklaeewkr nhdlqevvvt gigevqehlk krrmcqcseq
      121 qqvcnldvma avesseqrda pdeglpelaa a
//
LOCUS       XP_077508497             312 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119845 [Amblyomma americanum].
ACCESSION   XP_077508497
VERSION     XP_077508497.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652371.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..312
                     /product="uncharacterized protein LOC144119845"
                     /calculated_mol_wt=34539
     CDS             1..312
                     /gene="LOC144119845"
                     /coded_by="XM_077652371.1:1..939"
                     /db_xref="GeneID:144119845"
ORIGIN      
        1 mlirflffmk lvyciasvll sgdvetnpgp shsnpgskpg trstsssvnv qvastddacs
       61 tsnqpainem laellagqrk maediadikr fqqrvnsrfe aleprvsale sssptsngda
      121 sdgavqhqln slsevvskla srndelenrs rrnnllfhgl aeedeetnka lfiavtkvfi
      181 dnlqldcpki eryhrlgrkq egrprpvivk vldfrektqa lknahrlkns gislsedfsa
      241 hvrlirkkif datqsyrdsg skvhlrydhv fidsvrynwd dvekslvkds kstppdtaaa
      301 nataakppad sd
//
LOCUS       XP_077508512             414 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119865 [Amblyomma americanum].
ACCESSION   XP_077508512
VERSION     XP_077508512.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652386.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..414
                     /product="uncharacterized protein LOC144119865"
                     /calculated_mol_wt=43802
     Region          <201..>246
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     Region          240..>397
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..414
                     /gene="LOC144119865"
                     /coded_by="XM_077652386.1:22..1266"
                     /db_xref="GeneID:144119865"
ORIGIN      
        1 msshgaaiaa vagrgnrfas dadedyeivl ptlptgrvvl ntvflhgdvr arpyrvedfr
       61 dalanagalp dvvalgayqi nhvwavtfst adaakklaat kelevkgrrc ivfdpedhqv
      121 klrlhwmlhg vadedirtaf aafgnvtevt rehwrvagmk ekgsttrtvl lklkpgvkvd
      181 dlphqvrvag elalvvvsgr pmqclrcggt ghvrrdckvp rcskcrrygh seadcvrtya
      241 satghvaasv saelmdvvea eeaasgaddt gkaegvpvqp avassntdta vaqesgspat
      301 dpsstpvetp kdgasssvhv erkaaseshd ekddsrmnvg vttpakrpha etkadgekaa
      361 epsvgeppak tpqarraglr prpkvpaekr ggdkappdqe hvlapddtgg dgiv
//
LOCUS       XP_077508515             366 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119867 [Amblyomma americanum].
ACCESSION   XP_077508515
VERSION     XP_077508515.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652389.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..366
                     /product="uncharacterized protein LOC144119867"
                     /calculated_mol_wt=40532
     Region          <119..165
                     /region_name="transpos_IS481"
                     /note="IS481 family transposase; NF033577"
                     /db_xref="CDD:468094"
     CDS             1..366
                     /gene="LOC144119867"
                     /coded_by="XM_077652389.1:1..1101"
                     /db_xref="GeneID:144119867"
ORIGIN      
        1 mptygalepf hgeggawtey lervklffda nsvpeekkks vfltccgsst nsllrslltp
       61 ktpdqvaide ifsvlsghyi pkpsvvvcrf rfnsrsrqpe esvsdytasl kklsanyiiv
      121 sdngtaftsk kyadflskng irrvlippyh pesngtterv vqtikgklkk avpgdfqtni
      181 drillsyrtt phaftgslpa ellmgrqlqm piqrmhpglr ahadfkqlkq kmrcdskark
      241 sclpspgepv fvgnfrpgpa wiagtvrhpt sssslqiele dgtlwkrhad hvrprgtpgv
      301 lsydeepppa laattdteql spprcslste qsttasarqq qssaapvhpq lrrstrvrkp
      361 viyygl
//
LOCUS       XP_077508530             416 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119891 [Amblyomma americanum].
ACCESSION   XP_077508530
VERSION     XP_077508530.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652404.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..416
                     /product="uncharacterized protein LOC144119891"
                     /calculated_mol_wt=45973
     Region          <195..258
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     Region          <347..382
                     /region_name="ZIP_TSC22D-like"
                     /note="leucine zipper found in the TSC22 domain leucine
                     zipper transcription factors, c-Myc-binding protein, and
                     similar proteins; cl40461"
                     /db_xref="CDD:424092"
     Site            order(350,354..355,357..362,364..365,367..368,371..372,
                     374..375,378..379,381..382)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:409275"
     CDS             1..416
                     /gene="LOC144119891"
                     /coded_by="XM_077652404.1:1..1251"
                     /db_xref="GeneID:144119891"
ORIGIN      
        1 mdelrsrdhv nyargvaaav tkaarmpsim pkddiqiivr prgglniart easiimsaii
       61 aaagvpkmea neattctnva qniiavstpg qrraglylrm rglfiggksh evstyqaaqe
      121 davkgvirgv aiegspsein hnivnpanpl diqahrignt ttvivlskgp kvpnyvkygp
      181 illrcalyqk hfdvcrqcgk vghrsdvcpf pdtrvclacg eanpnadher hckpkcklcg
      241 gqhgtgarec tnkfkvsyvv krrqwnrkme dlrsqqlhrl pereadfppl qqtsvectrg
      301 rsqtrgssrq rsnserslsr nrqpsqtrer ivwadtlkep kiperrpsae yaaikalrae
      361 neqlkqkiaa lehtnksisa kldqllalqq qpqpappqpq lrdqppaepi tqgsmp
//
LOCUS       XP_077508549             159 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119918 [Amblyomma americanum].
ACCESSION   XP_077508549
VERSION     XP_077508549.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652423.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..159
                     /product="uncharacterized protein LOC144119918"
                     /calculated_mol_wt=17549
     CDS             1..159
                     /gene="LOC144119918"
                     /coded_by="XM_077652423.1:1..480"
                     /db_xref="GeneID:144119918"
ORIGIN      
        1 mvaccddsfs efvdfvrrae dvtsdeaysf vhplrrnlrd rqnpievhnn qgflaryrfy
       61 kaavlellsm lprhgntvgr gcpvppllql lvtlcfygag tiqvvsgglv nvsqptvsrv
      121 vtrvstmiaa tlysalvkfs eagkmhqamn dlnkiaqfp
//
LOCUS       XP_077508558             159 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119929 [Amblyomma americanum].
ACCESSION   XP_077508558
VERSION     XP_077508558.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652432.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..159
                     /product="uncharacterized protein LOC144119929"
                     /calculated_mol_wt=17643
     CDS             1..159
                     /gene="LOC144119929"
                     /coded_by="XM_077652432.1:1..480"
                     /db_xref="GeneID:144119929"
ORIGIN      
        1 mvaccddsfs efvdfvrrae dvtsdeaysl vhplrrnlrd rqnpievynn qgflaryrfy
       61 kaavlellsm lprhgntvgr gcpvppllql lvtlrfygag tiqvvsgglv nvsqptvsrv
      121 vtrvstmiaa tlysalvkfs eagkmhqamy dlnkiaqfp
//
LOCUS       XP_077508583             209 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119956 [Amblyomma americanum].
ACCESSION   XP_077508583
VERSION     XP_077508583.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652457.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..209
                     /product="uncharacterized protein LOC144119956"
                     /calculated_mol_wt=22639
     CDS             1..209
                     /gene="LOC144119956"
                     /coded_by="XM_077652457.1:35..664"
                     /db_xref="GeneID:144119956"
ORIGIN      
        1 mcdypsspdr ptaqlhttgs glhlaiegdh gqgadccrer srdiatcvtr sagklpvaev
       61 akryvasdrr ltltigeqga ddyhhnndyh kgrlgsekrm cgnasyraql savppatrtw
      121 sktstwasri aacavqtpqa kaqaamgrhd ipdavarras apasrcrkwh acalrlasll
      181 arkfvdiqsm fdvsswapei sfgsldlif
//
LOCUS       XP_077508590             182 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119966 [Amblyomma americanum].
ACCESSION   XP_077508590
VERSION     XP_077508590.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652464.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..182
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..182
                     /product="uncharacterized protein LOC144119966"
                     /calculated_mol_wt=19634
     CDS             1..182
                     /gene="LOC144119966"
                     /coded_by="XM_077652464.1:35..583"
                     /db_xref="GeneID:144119966"
ORIGIN      
        1 mcdypsspdr ptaqlhttgs glhlaiegdh gqgadccrer srdiatcvtr sagklpvaev
       61 akryvasdrr ltltigeqga deyhhnndyh kgrlgsekrm cgnasyraql savppatrtw
      121 sktstwasri aacavqtpqa kaqaamgrhd ipdavarras apasrcrkwh acalrlasyv
      181 fs
//
LOCUS       XP_077508596             195 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144119976 [Amblyomma americanum].
ACCESSION   XP_077508596
VERSION     XP_077508596.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652470.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..195
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..195
                     /product="uncharacterized protein LOC144119976"
                     /calculated_mol_wt=21062
     CDS             1..195
                     /gene="LOC144119976"
                     /coded_by="XM_077652470.1:1..588"
                     /db_xref="GeneID:144119976"
ORIGIN      
        1 mcdypsspdr ptaqlhttgs glhlaiegdh gqgadccrer srdiatcvtr sagklpvaev
       61 akryvasdrr ltltigeqga ddyhhnndyh kgrlgsekrm cgnasyraql savppatrtw
      121 sktstwasri aacavqtpqa kaqaamgrhd ipdavarras apasrcrkwh acalrlasll
      181 arkfvdiqsm fdvss
//
LOCUS       XP_077508612             844 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-4-like [Amblyomma americanum].
ACCESSION   XP_077508612
VERSION     XP_077508612.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652486.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..844
                     /product="protein argonaute-4-like"
                     /calculated_mol_wt=96067
     Region          283..342
                     /region_name="ArgoN"
                     /note="N-terminal domain of argonaute; pfam16486"
                     /db_xref="CDD:465134"
     Region          355..469
                     /region_name="PAZ"
                     /note="PAZ domain; pfam02170"
                     /db_xref="CDD:460472"
     Region          496..824
                     /region_name="Piwi-like"
                     /note="PIWI domain. Domain found in proteins involved in
                     RNA silencing. RNA silencing refers to a group of related
                     gene-silencing mechanisms mediated by short RNA molecules,
                     including siRNAs, miRNAs, and heterochromatin-related
                     guide RNAs. The central component...; cl00628"
                     /db_xref="CDD:412485"
     Site            order(628,632,644..647,650,659,662,666,670)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     CDS             1..844
                     /gene="LOC144119996"
                     /coded_by="XM_077652486.1:1..2535"
                     /db_xref="GeneID:144119996"
ORIGIN      
        1 mevvegdevd preftapgqw fqcrgkkvvp leksrreiea eqfrlrqagr klaafsvekq
       61 eaqipegadk nilrpksgld rllqrgpayl trmikasagv niqeteeedi vipntkqhsi
      121 liattsgerk mkyvsinlik lgeeeqvpvf ayvatpdgcg kgvvhgvdri ftqeeimqml
      181 rhkanpevig eiertlplhf pcrpaqgklg rpiqltanyf nieipagnvf yydveicset
      241 rkeakvpenr kyrcistkin rlviellvkk yrvdlngcmp afdgrknlyt rrepkfrtrt
      301 ftvdfeenqr iqkfivkiqy agtvnldalh avfdnrvsqv phgvpharag arcrpdfrel
      361 rdsqnvrlnk elkglrvkvt hlpyprkykv irvtkepakk lffnmedgfc cavadyfykr
      421 yhrlsypnlp ciqtgsathp vylplevcvi vegqhckkkl denqtsemik rtaqppakrf
      481 netrqtvrdl vsssdqclhe fgikisteqt qlrgrvldpp slvfennfig kpregtwelr
      541 grhfyepaml trwtllnlsr faqrdsldnf vrtlirvgqe lgmcieqpld vtttdanrkp
      601 vrnilleeqr ktvslemvii mlakntnyae ikqvaeteig lrtqcvmdsn vikkcnaali
      661 tnlcqkmnak ldginsllpk ekpkifqkpv iiigadvthp apgdklrlsi aacvgsldsi
      721 pskfhasiri qmedsaatsp wrssrisrvs airlacqels pnetyepalt ftvvqkrhht
      781 rfmptndrdg vgqcrnvppg ttvdsvvthp ldfdfylcsh fgnqvhsngm psgapsvnsr
      841 ywwl
//
LOCUS       XP_077508622             142 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120006 [Amblyomma americanum].
ACCESSION   XP_077508622
VERSION     XP_077508622.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652496.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 36% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..142
                     /product="uncharacterized protein LOC144120006"
                     /calculated_mol_wt=15750
     CDS             1..142
                     /gene="LOC144120006"
                     /coded_by="XM_077652496.1:1..429"
                     /db_xref="GeneID:144120006"
ORIGIN      
        1 mamslgsfal iyllsldtap erayerydfs tariflayle qrkrlkkkkf afltcfacsc
       61 cslvllvvpd cslvlvwrda gaarfqfdlv kpilrpslsp adyvdssgsp gnvftegpcd
      121 srslmnaswa saegnarrrk qf
//
LOCUS       XP_077508629             132 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120018 [Amblyomma americanum].
ACCESSION   XP_077508629
VERSION     XP_077508629.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652503.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 23% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..132
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..132
                     /product="uncharacterized protein LOC144120018"
                     /calculated_mol_wt=14066
     CDS             1..132
                     /gene="LOC144120018"
                     /coded_by="XM_077652503.1:330..728"
                     /db_xref="GeneID:144120018"
ORIGIN      
        1 mhpaghsrqp slqrparqrs srgsrcqwsk afglrsvhat lcggdsqgtt qvaiafvmma
       61 lqldleggpa allllplpva saagpqvhah prsfrlvpgs hgeswralls lefvgirsaf
      121 grpwavtrar rs
//
LOCUS       XP_077508634             130 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120026 [Amblyomma americanum].
ACCESSION   XP_077508634
VERSION     XP_077508634.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652508.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..130
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..130
                     /product="uncharacterized protein LOC144120026"
                     /calculated_mol_wt=14210
     CDS             1..130
                     /gene="LOC144120026"
                     /coded_by="XM_077652508.1:1..393"
                     /db_xref="GeneID:144120026"
ORIGIN      
        1 meydlgqrad cchersrnia trvtrpagkp pvaevtkrfv ayvrrlglaf geqgagpyyh
       61 hdynkgrfgs ekrvcgnapf qaqppaippe tgtwsktstw gprrrrmcga dsagsspgfh
      121 grpcmtaetl
//
LOCUS       XP_077508645             347 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120044 [Amblyomma americanum].
ACCESSION   XP_077508645
VERSION     XP_077508645.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652519.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 45% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..347
                     /product="uncharacterized protein LOC144120044"
                     /calculated_mol_wt=36624
     Region          <30..>128
                     /region_name="PTZ00249"
                     /note="variable surface protein Vir28; Provisional"
                     /db_xref="CDD:140276"
     CDS             1..347
                     /gene="LOC144120044"
                     /coded_by="XM_077652519.1:1..1044"
                     /db_xref="GeneID:144120044"
ORIGIN      
        1 msgaavssvv dgrgaayrqp plgpvpseqp vprghlgshr tpssksaslp rvhtgpvpse
       61 qpvprghlgs hrtpssksas lprghgaplp sekpvlrfll gshrtpssqs assprghtra
      121 vpteqpvphf hlgshrtpss psassprgha apmedktlvt ttttsvakak rfklreapaq
      181 kctqpvtavs lpplpagqrr nprtasaeeq ditfrfthrh vsqaavarra sdrgiqysfl
      241 cqmvdqdkps ervqlstqsq wvaallsspr arrfdsavtg raeqvaerhr qsaicfglee
      301 epaagskvtg slrgeasppq aapnasssik ftsssssqkd pgsssse
//
LOCUS       XP_077508653             297 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120053 [Amblyomma americanum].
ACCESSION   XP_077508653
VERSION     XP_077508653.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652527.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 21% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..297
                     /product="uncharacterized protein LOC144120053"
                     /calculated_mol_wt=31813
     Region          <82..>180
                     /region_name="PTZ00249"
                     /note="variable surface protein Vir28; Provisional"
                     /db_xref="CDD:140276"
     CDS             1..297
                     /gene="LOC144120053"
                     /coded_by="XM_077652527.1:1..894"
                     /db_xref="GeneID:144120053"
ORIGIN      
        1 msinpddiii iiiiiiissr llelsmlert ilakeltaav raratgtsqp vprfhlgshr
       61 tpisqsalsp rghagpvpse qpvprghlgs hrtpssksas lprvhtgpvp seqpvprghl
      121 gshrtpssks aslprghgap lpsekpvlrf llgshrtpss qsassprght ravpteqpvp
      181 hfhlgshrtp sspsassprg haapmedktl vtttttsvak akrfklreap aqkctqpvta
      241 vslpplpagq rrnprtasae eqditfrfth rhvsqaavar rasdrgiqys flcqmve
//
LOCUS       XP_077508662             180 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120068 [Amblyomma americanum].
ACCESSION   XP_077508662
VERSION     XP_077508662.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652536.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 18% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..180
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..180
                     /product="uncharacterized protein LOC144120068"
                     /calculated_mol_wt=19202
     CDS             1..180
                     /gene="LOC144120068"
                     /coded_by="XM_077652536.1:1..543"
                     /db_xref="GeneID:144120068"
ORIGIN      
        1 milakeltaa vsaraagtsq pmprfhlcsh rtpssqsass prghagpvps eqpvprfqld
       61 rhqtpssksa slprghgapv pseqpvphfh lgshrtpssp sassprghaa pmedktlvtt
      121 tttsvakakr ylssekrlrr narsrsqpla fphylqanda alaprllrsk tlpsaprivt
//
LOCUS       XP_077508671             130 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120078 [Amblyomma americanum].
ACCESSION   XP_077508671
VERSION     XP_077508671.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652545.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..130
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..130
                     /product="uncharacterized protein LOC144120078"
                     /calculated_mol_wt=14210
     CDS             1..130
                     /gene="LOC144120078"
                     /coded_by="XM_077652545.1:1..393"
                     /db_xref="GeneID:144120078"
ORIGIN      
        1 meydlgqrad cchersrnia trvtrpagkp pvaevtkrfv ayvrrlglaf geqgagpyyh
       61 hdynkgrfgs ekrvcgnapf qaqppaippe tgtwsktstw gprrrrmcga dsagsspgfh
      121 grpcmtaetl
//
LOCUS       XP_077508710             675 aa            linear   INV 25-NOV-2025
DEFINITION  high-affinity choline transporter 1-like [Amblyomma americanum].
ACCESSION   XP_077508710
VERSION     XP_077508710.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652584.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..675
                     /product="high-affinity choline transporter 1-like"
                     /calculated_mol_wt=71475
     Region          23..501
                     /region_name="SLC5sbd_CHT"
                     /note="Na(+)- and Cl(-)-dependent choline cotransporter
                     CHT and related proteins; solute-binding domain; cd11474"
                     /db_xref="CDD:271368"
     Site            order(76,79,351,354..355)
                     /site_type="other"
                     /note="Na binding site [ion binding]"
                     /db_xref="CDD:271368"
     CDS             1..675
                     /gene="LOC144120149"
                     /coded_by="XM_077652584.1:1..2028"
                     /db_xref="GeneID:144120149"
ORIGIN      
        1 melhdvgpta tatteaprnt paiavlvafy aatlavaiwa knrvqdpddd gasnlcqfll
       61 vkrglgpllg tatmtatwig ggyvhsaaes iyryglvwcq apfgyacslf vggsffavkm
      121 raaeyetmld pfqehfgpgm gallfipafm gelfwmsaif salgatlhvs lgishneail
      181 gpafllmfyt ftggfcavay tdalqlsatv iglwaavpfi vlhpsvdkvs ypannwlggv
      241 eaeyyafyvd rmlmitlggi cwqvyfqril asrtpevsli vslasafgcl fvaapaviig
      301 aaaraanftq agfegpsplp aedvprvlpl smlhlsptiv ssvgltgvsa avmasadssi
      361 lsaasmfsqn vykvllrdqa sleemnlvir lavvvvgima tyisqavgav qglwylssdm
      421 vyvvlfpqlv cvayfrdlsn tygsaaayvl avmlrvgage kalslpplip ypyydeqtgr
      481 qlfpvrtftm lasliillav shlstwlfta rhlplrydvf gcfqldnall skpcakkall
      541 fkhppsrpqg feasvvgtta vsscedplsa dsiaatphqt ggrakskepp avvaaapghs
      601 vprpvadapq adlspqsasv asvgslepag vppvavqeak eepksegkkk krrkkakpsd
      661 dskgaaaaae sgsfp
//
LOCUS       XP_077508715             237 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120158 [Amblyomma americanum].
ACCESSION   XP_077508715
VERSION     XP_077508715.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652589.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..237
                     /product="uncharacterized protein LOC144120158"
                     /calculated_mol_wt=27317
     Region          <1..44
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     CDS             1..237
                     /gene="LOC144120158"
                     /coded_by="XM_077652589.1:29..742"
                     /db_xref="GeneID:144120158"
ORIGIN      
        1 mqinvsktkt itfttnnqlq nhcysmnsil ienvstvkyl gvhfssdltw knhidaivsk
       61 sskalgfirr hlhqahpaak flayttlvrs eieyasliws phqlylihkl eaiqnkaarf
      121 itknysrqss itainaslkl pslekrrila llshihrlyh shssfasyyi rpthrifprl
      181 dhalkielmf arthlfshsp lflavhhwnk lpsdivftln hdafvqklkg flqinsd
//
LOCUS       XP_077508719             305 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120167 [Amblyomma americanum].
ACCESSION   XP_077508719
VERSION     XP_077508719.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652593.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 28% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..305
                     /product="uncharacterized protein LOC144120167"
                     /calculated_mol_wt=34169
     CDS             1..305
                     /gene="LOC144120167"
                     /coded_by="XM_077652593.1:1..918"
                     /db_xref="GeneID:144120167"
ORIGIN      
        1 mvlrvdavpv vqparrvpla lreplreelq rmeqggiiak vteptdwrph tsrparltep
       61 lysnrpktrd wrpllrrqap tqlarlrrrr avvalkrrgd qrerhqhggq qqhpgsalls
      121 iringmtplr ggarsdewrg eprppkplpg rrggglgapa agrtprvvrw ravagrssgd
      181 thgtqqqqqq appprtsgap pgpdrrrrrw easapsrdas dprratrcgr reqalgprrr
      241 tpcapgfpwr pphrrtrppr epswttagsy rggltralgr paglsplepr gryggrrpvp
      301 rrial
//
LOCUS       XP_077508728             921 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077508728
VERSION     XP_077508728.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652602.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..921
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=102326
     Region          133..891
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          523..892
                     /region_name="Piwi-like"
                     /note="PIWI domain. Domain found in proteins involved in
                     RNA silencing. RNA silencing refers to a group of related
                     gene-silencing mechanisms mediated by short RNA molecules,
                     including siRNAs, miRNAs, and heterochromatin-related
                     guide RNAs. The central component...; cl00628"
                     /db_xref="CDD:412485"
     Site            order(655,659,671..674,677,686,689,693,697)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     CDS             1..921
                     /gene="LOC144120177"
                     /coded_by="XM_077652602.1:1..2766"
                     /db_xref="GeneID:144120177"
ORIGIN      
        1 mgqavpsasg gnqatppaps pagpswvsfa qalqqgaaas pqpravsggp ttaadpsahp
       61 taqpsasrps ygaaaaagsa kvasisprpa saaaavsgtq paaaalppsp pstppvavtq
      121 qtpaagdqat sgddvrikel ertlpshfpr rpahgqlgrt iqllanhfsi eiptgsvyhy
      181 dvdissetak etkvpeqkny rclstkinri vielvvkkyr qdlancipaf dgrknlytrr
      241 qlnfrertft vdleedqrsq kfiikiqyaa tvnldalhgv fqkrvqtvpq evlqaihivl
      301 rhspsinlap vgrlffrppg pnehndlggg relwfgyyas vrpaqwkpml nvdmsatafy
      361 eslplvdfmc rffsdsrrvs tpadlrslrd nqyarlnrel kglrvkvthl pyprkykvvk
      421 itreaakdiy fesegsqisv adyfqsryrr lsypnfpcvq sgspthpvyi plevcelaeg
      481 qhcrkklees qtaemikraa kppakrflei rqsvrdmvst sdkylrefgv kinpeptqvv
      541 grildppslv fennsickpr dgtwdlrgqr fykamsmtqw ivlnvsrfaq kdcldnfikm
      601 lirigqelgm riaqplavit fdtnrkpmrt vlteqrkqhp qlemvvavit katnyaeikq
      661 vaetelslrt qcildnnvvq kcnaaliqnl cqkinakmgg innsllmqek pkllhrpviv
      721 igadvshpsp gdkirpsiaa cvgsldsvps kfhatirvqi edskakacve iikdlkdmik
      781 elllvfyrtt ghkplrivfy rdgvsegqfl evrnhevsai rlacaemcpt etyeppltfi
      841 vvqkrhhtrf mpandrdgvg kfrnvppgtt vdsvvthpld fdfflcshfg iqvgdlfsal
      901 vaivsgkkws avfsfinlrr k
//
LOCUS       XP_077508736             183 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077508736
VERSION     XP_077508736.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652610.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=20202
     Region          <4..139
                     /region_name="Piwi-like"
                     /note="PIWI domain. Domain found in proteins involved in
                     RNA silencing. RNA silencing refers to a group of related
                     gene-silencing mechanisms mediated by short RNA molecules,
                     including siRNAs, miRNAs, and heterochromatin-related
                     guide RNAs. The central component...; cl00628"
                     /db_xref="CDD:412485"
     CDS             1..183
                     /gene="LOC144120187"
                     /coded_by="XM_077652610.1:1..552"
                     /db_xref="GeneID:144120187"
ORIGIN      
        1 mkgvsairla ckelspnety epaitfivvq krhhtrfmpa ndrdgvgkcr nvppgttvds
       61 vvthpldfdf flcshfgiqg tsrpahyyvv wddskfsadd lqkhsfylch tysrcarsvs
      121 ipapvyyahl aafrakhhia skletsgavs qssegggdtv lttaqyveav kvlqelqasm
      181 yfv
//
LOCUS       XP_077508742            1108 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077508742
VERSION     XP_077508742.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652616.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 14% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1108
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=123032
     Region          222..299
                     /region_name="ArgoN"
                     /note="N-terminal domain of argonaute; pfam16486"
                     /db_xref="CDD:465134"
     Region          309..360
                     /region_name="ArgoL1"
                     /note="Argonaute linker 1 domain; pfam08699"
                     /db_xref="CDD:462567"
     Region          363..477
                     /region_name="PAZ_argonaute_like"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; cd02846"
                     /db_xref="CDD:239212"
     Site            order(415,430,439,443,460,467,469)
                     /site_type="other"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]"
                     /db_xref="CDD:239212"
     Region          522..951
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(654,658,670..673,676,685,688,692,696)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(723,725,801,941)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..1108
                     /gene="LOC144120197"
                     /coded_by="XM_077652616.1:1..3327"
                     /db_xref="GeneID:144120197"
ORIGIN      
        1 mssvpsasgg nqatppapsp agpswvsfaq alqqgaaasp qpravsggpt taadpsahpt
       61 aqpsasrpsy gaaaaagsak vasisprpas aaaavsgtqp aaaalppspp stppvavtqq
      121 tpaagdqats gddvrikele rtlpshfprr pahgqlgrti qllanhfsie iptgsvyhyd
      181 vdissetake tkvpeqknyr clstkinriv ielvvkkyrq dlancipafd grknlytrrq
      241 lnfrertftv dleedqrsqk fiikiqyaat vnldalhgvf qkrvqtvpqe vlqaihivlr
      301 hspsinlapv grlffrppgp nehndlgggr elwfgyyasv rpaqwkpmln vdmsatafye
      361 slplvdfmcr ffsdsrrvst padlrslrdn qyarlnrelk glrvkvthlp yprkykvvki
      421 treaakdiyf esegsqisva dyfqsryrrl sypnfpcvqs gspthpvyip levcelaegq
      481 hcrkkleesq taemikraak ppakrfleir qsvrdmvsts dkylrefgvk inpeptqvvg
      541 rildppslvf ennsickprd gtwdlrgqrf ykamsmtqwi vlnvsrfaqk dcldnfikml
      601 irigqelgmr iaqplavitf dtnrkpmrtv lteqrkqhpq lemvvavitk atnyaeikqv
      661 aetelslrtq cildnnvvqk cnaaliqnlc qkinakmggi nnsllmqekp kllhrpvivi
      721 gadvshpspg dkirpsiaac vgsldsvpsk fhatirvqie dskakarvei ikdlkdmike
      781 lllvfyrttg hkplrivfyr dgvsegqffe vrnhevsair lacaemcpte tyeppltfiv
      841 vqkrhhtrfm pandrdgvgk frnvppgttv dsvvthpldf dfflcshfgi qgtsrpahyy
      901 ivwddsnfsa ddlqklsfyl chtysrcars vsipapvyya hlaafrakhh iaskletgap
      961 cndgaaakrs hcpldnkpfq egdvafsaer csemvlhqdi dehlesdcvp srarkpddtf
     1021 dnvvmqvkea lgkiqeenaa lrmkldsveq clrpetentv apqstiiasa vtdalenkis
     1081 vlktlaetal aehqrevase ithavagn
//
LOCUS       XP_077508749             282 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120207 [Amblyomma americanum].
ACCESSION   XP_077508749
VERSION     XP_077508749.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652623.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 26% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..282
                     /product="uncharacterized protein LOC144120207"
                     /calculated_mol_wt=29307
     Region          <3..>88
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     Region          149..>205
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          <242..>277
                     /region_name="Piwi-like"
                     /note="PIWI domain. Domain found in proteins involved in
                     RNA silencing. RNA silencing refers to a group of related
                     gene-silencing mechanisms mediated by short RNA molecules,
                     including siRNAs, miRNAs, and heterochromatin-related
                     guide RNAs. The central component...; cl00628"
                     /db_xref="CDD:412485"
     CDS             1..282
                     /gene="LOC144120207"
                     /coded_by="XM_077652623.1:1..849"
                     /db_xref="GeneID:144120207"
ORIGIN      
        1 mtdavpsasg gdqatapaps pagatapaps pagpsrvlfa qalqqgavps pqpravsrgp
       61 tpaaepraqp taqptaqpta kpsayrpsyg aaaaagdanv aassprpasa aaavsrsqpa
      121 aaalppsppc tppvavteqt peagdqatpg ddvrikeler tlpshfprrp ahgqlgrtiq
      181 llanhfsiei ptgsvyhydv dissetaket kvpeqknyrc lstkikrivi elviedskak
      241 acveiikdlk dmikelllvf yrttghkplr ivfyrdgals sp
//
LOCUS       XP_077508779             202 aa            linear   INV 25-NOV-2025
DEFINITION  superkiller complex protein 2-like [Amblyomma americanum].
ACCESSION   XP_077508779
VERSION     XP_077508779.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652653.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..202
                     /product="superkiller complex protein 2-like"
                     /calculated_mol_wt=23134
     Region          <11..54
                     /region_name="rRNA_proc-arch"
                     /note="rRNA-processing arch domain; pfam13234"
                     /db_xref="CDD:463813"
     Region          <113..185
                     /region_name="DSHCT"
                     /note="DSHCT (NUC185) domain; pfam08148"
                     /db_xref="CDD:462374"
     CDS             1..202
                     /gene="LOC144120251"
                     /coded_by="XM_077652653.1:866..1474"
                     /db_xref="GeneID:144120251"
ORIGIN      
        1 metvdfvncs qqleekllen kcltcllfea hfeqehrrrr lseevrhlqr qlsqdslafm
       61 pdyrnhvlal erlgyvepsg tltlkrhvar slsshevmlt elllqesllt lvapevaglf
      121 fcfvyeqrsn sepvvprsmk ahfahimqlt vvqegiivrc iqrldellkd fktavgivgn
      181 pelrampmsa vvfpksvvrk fs
//
LOCUS       XP_077508786             417 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120260 [Amblyomma americanum].
ACCESSION   XP_077508786
VERSION     XP_077508786.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652660.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 44% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..417
                     /product="uncharacterized protein LOC144120260"
                     /calculated_mol_wt=46448
     Region          162..207
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          243..402
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..417
                     /gene="LOC144120260"
                     /coded_by="XM_077652660.1:1..1254"
                     /db_xref="GeneID:144120260"
ORIGIN      
        1 midhvicfiq eppetaqads qhppdsdtsa eppgaaqads qqfqdtdtst qppeealads
       61 qhfqetdtst vptqaaradv pqvqnedtvt vskrdkstql eeighnghty skcglqhifg
      121 kqqglssnft eedcqfytgl skpvfdslvk aatiesnktf lklsvedqll lsimrmrfgl
      181 fyghlarifv lfisyvhklf iytlsvlqkv mkkvvvwlpr slirnsmpes ftlngyektt
      241 giidcsevfh qrpekllara qtyssykvhn tvkfltaiap ngymmfvsra yggrasdkfi
      301 msdcgvedli gpgdevmadr gfvlstnldi qgvklnvpsf trgksqlses eetqtsrias
      361 vrihverain rtktfrifkh alpirskkti sdmifvcggl cnlkgpliar svegrde
//
LOCUS       XP_077508802             233 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120277 [Amblyomma americanum].
ACCESSION   XP_077508802
VERSION     XP_077508802.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652676.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..233
                     /product="uncharacterized protein LOC144120277"
                     /calculated_mol_wt=26206
     CDS             1..233
                     /gene="LOC144120277"
                     /coded_by="XM_077652676.1:1..702"
                     /db_xref="GeneID:144120277"
ORIGIN      
        1 maaykrmlvq tertssssgn csqdivsiln ptttgalvge ssmltdmrrs silqaddhny
       61 tyridcpesl safvgavvpy iagfvvqkvr statcelcia alhsdelapl frqksrggli
      121 spsqdvvglc eavekglwll qaeydtikmv tvrskhlile vlgtcteknw fqkledhild
      181 fdpldnhiyi lckkiaeeyi kvrihhmtke rnreliknkv rpllsrviif nhl
//
LOCUS       XP_077508815             302 aa            linear   INV 25-NOV-2025
DEFINITION  superkiller complex protein 2-like [Amblyomma americanum].
ACCESSION   XP_077508815
VERSION     XP_077508815.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652689.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 12% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..302
                     /product="superkiller complex protein 2-like"
                     /calculated_mol_wt=34240
     Region          <31..118
                     /region_name="rRNA_proc-arch"
                     /note="rRNA-processing arch domain; pfam13234"
                     /db_xref="CDD:463813"
     Region          <177..302
                     /region_name="DSHCT"
                     /note="DSHCT (NUC185) domain; pfam08148"
                     /db_xref="CDD:462374"
     CDS             1..302
                     /gene="LOC144120296"
                     /coded_by="XM_077652689.1:1..909"
                     /db_xref="GeneID:144120296"
ORIGIN      
        1 mnaavrlqlw sadwitppss dasqsiqpqc rnvlgqelle lvethpggpp vlnvvkdlhl
       61 pametvdfvn csqqleekll enkcltcllf eahisfeqeh rrrrlseear hlqrqlseds
      121 lafmpdyrnh vlalerlgyv epsgtltlkr hvarslsshe vmltelllqe slltlvapev
      181 aglffcfvye qrsnsepvvp rsmkaaiekf vevavkighv qresgfdeps eqfleqfsfg
      241 lynvvyhwak gmhfahimql tvvqegiivr ciqrldellk dfktavgivg npelramvre
      301 as
//
LOCUS       XP_077508829             417 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120320 [Amblyomma americanum].
ACCESSION   XP_077508829
VERSION     XP_077508829.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652703.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 44% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..417
                     /product="uncharacterized protein LOC144120320"
                     /calculated_mol_wt=46448
     Region          162..207
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          243..402
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..417
                     /gene="LOC144120320"
                     /coded_by="XM_077652703.1:1..1254"
                     /db_xref="GeneID:144120320"
ORIGIN      
        1 midhvicfiq eppetaqads qhppdsdtsa eppgaaqads qqfqdtdtst qppeealads
       61 qhfqetdtst vptqaaradv pqvqnedtvt vsrrdkstql eeighnghty skcglqhifg
      121 kqqglssnft eedcqfytgl skpvfdslvk aatiesnktf lklsvedqll lsimrmrfgl
      181 fyghlarifv lfisyvhklf iytlsvlqkv mkkvvvwlpr slirnsmpes ftlngyektt
      241 giidcsevfh qrpekllara qtyssykahn tvkfltaiap ngymmfvsra yggrasdkfi
      301 msdcgvedli gpgdevmadr gfvlstnldi qgvklnvpsf trgksqlses eetqtsrias
      361 vrihverain rtktfrifkh alpirskkti sdmifvcggl cnlkgpliar svegrde
//
LOCUS       XP_077508846             334 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120354 [Amblyomma americanum].
ACCESSION   XP_077508846
VERSION     XP_077508846.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652720.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 47% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..334
                     /product="uncharacterized protein LOC144120354"
                     /calculated_mol_wt=36554
     Region          162..331
                     /region_name="TBP_eukaryotes"
                     /note="eukaryotic TATA box binding protein (TBP): Present
                     in archaea and eukaryotes, TBPs are transcription factors
                     that recognize promoters and initiate transcription. TBP
                     has been shown to be an essential component of three
                     different transcription initiation...; cd04516"
                     /db_xref="CDD:239952"
     Site            order(162,164,191,193,197,199,206,208,212,214,216,218,248,
                     253,255,284..285,290,301,303,305,309)
                     /site_type="other"
                     /note="DNA interaction surface [nucleotide binding]"
                     /db_xref="CDD:239952"
     Site            order(176,183..185,187,193,199,201)
                     /site_type="other"
                     /note="TFIIA interaction surface [polypeptide binding]"
                     /db_xref="CDD:239952"
     Site            order(191,289..290,295,326..327)
                     /site_type="other"
                     /note="NC2 interaction surface [polypeptide binding]"
                     /db_xref="CDD:239952"
     Site            order(265..266,278..282,285,290)
                     /site_type="other"
                     /note="TFIIB interaction surface [polypeptide binding]"
                     /db_xref="CDD:239952"
     CDS             1..334
                     /gene="LOC144120354"
                     /coded_by="XM_077652720.1:1..1005"
                     /db_xref="GeneID:144120354"
ORIGIN      
        1 mssaedrvaa essacsgvns aenpaptscp pkknacddve dvflsigpst psgddylaek
       61 rhlqdlaryl daftvaehst aaaavrphqt pgdastveew legssrkals wrptavpctp
      121 qeaaarddav atpstvraid dsavkreavg elqlllfahr rnvvatvnlc cqldlrdial
      181 rahnaeynpe hciaavimri reprttallf rsgkivctga rselqariaa rkfaritqkl
      241 gynarftdfk vqnivascdv gfriklesla liesqfscye pelfpglvyr meqpsvlllv
      301 fvsgkvvltg akvkehiyqa fdkiypklqn hrkv
//
LOCUS       XP_077508864             146 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120387 [Amblyomma americanum].
ACCESSION   XP_077508864
VERSION     XP_077508864.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652738.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..146
                     /product="uncharacterized protein LOC144120387"
                     /calculated_mol_wt=15773
     CDS             1..146
                     /gene="LOC144120387"
                     /coded_by="XM_077652738.1:435..875"
                     /db_xref="GeneID:144120387"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrppfa altsvlccqr pasigpcvlp afgcvippdp
       61 fmlktkscme vvqatklrvi fardleqagk lvdgqwysls kasfssktkt iflgswamyl
      121 gtcgqdnagq hasasskkkh etlnky
//
LOCUS       XP_077508872             131 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120403 [Amblyomma americanum].
ACCESSION   XP_077508872
VERSION     XP_077508872.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652746.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..131
                     /product="uncharacterized protein LOC144120403"
                     /calculated_mol_wt=14881
     CDS             1..131
                     /gene="LOC144120403"
                     /coded_by="XM_077652746.1:1..396"
                     /db_xref="GeneID:144120403"
ORIGIN      
        1 megnlglkdt rkllrcllep entedfqqrn airivhkldv ddstliknlk yryltttart
       61 tlpeysgppn peldedistw evraalhklr ttsapggdki tnktlrnlde nsiqavtklf
      121 nkhwregtlp p
//
LOCUS       XP_077508897             206 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120417 [Amblyomma americanum].
ACCESSION   XP_077508897
VERSION     XP_077508897.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652771.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..206
                     /product="uncharacterized protein LOC144120417"
                     /calculated_mol_wt=21965
     CDS             1..206
                     /gene="LOC144120417"
                     /coded_by="XM_077652771.1:46..666"
                     /db_xref="GeneID:144120417"
ORIGIN      
        1 mmrrgalcvh psspdrptaq rhptgsglhl ameddlgqgt eccrersrni aarvtrpagk
       61 ppvaevakrf vasvrrltlt igkqgaddhh hndyrkeslg sekrvfgnag cqaqpsavpp
      121 atgtpitpss nplpveqeic fgpthrrlcg adsagngpgl lplrqggqda lprvcpaefe
      181 maacaadkpg dkflliisyf willic
//
LOCUS       XP_077508911             227 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120421 [Amblyomma americanum].
ACCESSION   XP_077508911
VERSION     XP_077508911.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652785.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..227
                     /product="uncharacterized protein LOC144120421"
                     /calculated_mol_wt=24519
     CDS             1..227
                     /gene="LOC144120421"
                     /coded_by="XM_077652785.1:6..689"
                     /db_xref="GeneID:144120421"
ORIGIN      
        1 mqddlsqgtd rccesescry iatcaalpsg qtpdaelekr fvnsgprrts tigatfaalp
       61 sgqtpdaele krfvaseprs tstigatcaa lpsgqppdae lakrfvaswp cstngrqdag
      121 ddhhhlrgkg eqilklreap aqkctqpvta vslpplparq rrnprtasae eqditfrsty
      181 rhvsqaavar rasdrgiqfc clgqlvevvv tvtsdddirl rlnsdrd
//
LOCUS       XP_077508925             206 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120426 [Amblyomma americanum].
ACCESSION   XP_077508925
VERSION     XP_077508925.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652799.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..206
                     /product="uncharacterized protein LOC144120426"
                     /calculated_mol_wt=21965
     CDS             1..206
                     /gene="LOC144120426"
                     /coded_by="XM_077652799.1:46..666"
                     /db_xref="GeneID:144120426"
ORIGIN      
        1 mmrrgalcvh psspdrptaq rhptgsglhl ameddlgqgt eccrersrni aarvtrpagk
       61 ppvaevakrf vasvrrltlt igkqgaddhh hndyrkeslg sekrvfgnag cqaqpsavpp
      121 atgtpitpss nplpveqeic fgpthrrlcg adsagngpgl lplrqggqda lprvcpaefe
      181 maacaadkpg dkflliisyf willic
//
LOCUS       XP_077508939             169 aa            linear   INV 25-NOV-2025
DEFINITION  17S U2 SnRNP complex component HTATSF1-like [Amblyomma americanum].
ACCESSION   XP_077508939
VERSION     XP_077508939.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652813.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 14% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..169
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..169
                     /product="17S U2 SnRNP complex component HTATSF1-like"
                     /calculated_mol_wt=18229
     Region          110..>169
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..169
                     /gene="LOC144120433"
                     /coded_by="XM_077652813.1:1..510"
                     /db_xref="GeneID:144120433"
ORIGIN      
        1 msspsavdrd lfdlgsvvsv fliaaqrcsp plslllvted flaayqanyg ttdeappegs
       61 gdassssqkp pvpeksasgs nysaadpkqt ksgkakpsep swfelddahn twayvsnlpd
      121 gideeeflel mskcgllkkd ekghykiely rtregelkgd alcfyikva
//
LOCUS       XP_077508953             817 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 2-like [Amblyomma americanum].
ACCESSION   XP_077508953
VERSION     XP_077508953.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652827.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..817
                     /product="endothelin-converting enzyme 2-like"
                     /calculated_mol_wt=89189
     Region          114..741
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..817
                     /gene="LOC144120437"
                     /coded_by="XM_077652827.1:1..2454"
                     /db_xref="GeneID:144120437"
ORIGIN      
        1 mngqlavphr hewwaqrmws rmagegceap sttsggldrr qravalghll ssllgvaltl
       61 saitiglsvl rhqrgsegsg afgmgrsswn tcnsklcmah frrlkdslnr stapcgdfyr
      121 facgawhpra aksetafryl lavaqqdair yleavndasn kmvlptgkvl lpdsrpvlri
      181 nraerayqvc lhsgsstkda alltefladr slswpehtta paqpldvlld qdinwnlglw
      241 fsvrvspipg rnqrqvrvgs givsydwkqa ldtmvtrgvy karvrrfhda lrarsfaams
      301 dkemeelrry estivgalcg svsggqnela fplkqmqnla tprlataqwd tllnwqlnka
      361 lrvteadrvi atnvkllqav dyllsslpae vvlhqlawsl vqmigwmads alpgrptaye
      421 medmrsadcf wvthrafgvt hlsgyinaty plqwrssvda ilmtitqtai nfflktswih
      481 aesraaavdk lrrmntslwp sdsylnasev aailenfpep redgavlryw ldacrlaers
      541 lagsgmwptp stletcsrrg pcsattivsl halrsplfve gvglavnmgg lgahygvala
      601 rafdprgvll dgrgstsglw cgkssyrdye rrtacpataa sgaeggvqaf egvaaieiay
      661 gafnasrsia dqgtrthqrr rvflglsedq affvafcqas cargpserkq lsctlplknf
      721 refataftcp vaspmnpalr cgffeasdqr aarknggese dagksastna sanvgadgav
      781 tswtrpparr sgsddqdplt lattglaghm vrqppvl
//
LOCUS       XP_077508966             802 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 2-like [Amblyomma americanum].
ACCESSION   XP_077508966
VERSION     XP_077508966.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652840.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..802
                     /product="endothelin-converting enzyme 2-like"
                     /calculated_mol_wt=87687
     Region          93..726
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..802
                     /gene="LOC144120443"
                     /coded_by="XM_077652840.1:1..2409"
                     /db_xref="GeneID:144120443"
ORIGIN      
        1 magegceaps ttsggldrrq ravalghlls sllgvaltls aitiglsvlr hqrgsegsga
       61 fgmgrsswnt cnsklcmahf rrlkdslnrs tapcgdfysf acgawhpraa ksetafrylq
      121 avaqqdairy leavndasnk mvlptgkvll pdsrpvlrin raerayqvcl hsgsstkdaa
      181 lltefladrs lswpehttap aqpldvlldq dinwnlglwf svrvspipgr nqrqvrvgsg
      241 ivsydwkral dtmvtrgvyk arvrrfhdal rarsfaamsd kemeelrrye stivgalcgs
      301 vsggqnelaf plkqmqnlat prlataqwdt vlnwqlnkal rvteadrvia tnvkllqavd
      361 yllsslpaev vlhqlawslv qmigwmadsa lpgrptayem edmrsadcfw vthrafgvth
      421 lsgyinttyp lqwrssvdai lmtitqtain fflktswiha esraaavdkl rrmntslwps
      481 dsylnaseva ailenfpepr edgavlrywl dalsarrall grqwdvpdaf ypgdlqppra
      541 lfryhyylne lavslhalrs plfvegvgla vnmgglgahy avalarafdp rgvlldgrgs
      601 tsglwcgkss yrdyerrtac pataasgaeg gvqafegvaa ieiaygafna srsiadqgtr
      661 thqrrrvflg lsedqaffva fcqascargp serkqlsctl plknfrefat aftcpvaspm
      721 npalrcgffe asdqraarkn ggesedagks astnasanvg adgavtswtr pparrsgsdd
      781 qdpltlattg laghmvrqpp vl
//
LOCUS       XP_077508978             802 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 2-like [Amblyomma americanum].
ACCESSION   XP_077508978
VERSION     XP_077508978.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652852.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..802
                     /product="endothelin-converting enzyme 2-like"
                     /calculated_mol_wt=87719
     Region          93..726
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..802
                     /gene="LOC144120450"
                     /coded_by="XM_077652852.1:1..2409"
                     /db_xref="GeneID:144120450"
ORIGIN      
        1 magegceaps ttsggldrrq ravalghlls sllgvaltls aitiglsvlr hqrgsegsgv
       61 fgmgrsswnt cnsklcmahf rrlkdslnrs tapcgdfyrf acgawhpraa ksetvfrdll
      121 avaqqdairy leavndalnk mvlptgkvll pdsrpvlrin raerayqvcl hsgsstkdaa
      181 lltefladrs lswperttap aqpldvlldl dinwnlglwv svrvspipgr nqrqvrvgsg
      241 ivsydwkral dtmvmrgvyk arerlfhdal rarsfaamsd kemeelrrye stivgalcgs
      301 vsggqnelal plkqmqnlat prlataqwdt vlnwqlnkal rvteadrvia tnvkllqavd
      361 yllsslpaev vlhqlawslv qmigwmadsa lpgrptayem edmrsadcfw vthrafgvth
      421 lsgyinttyp lhwrssvdai lmtitqtain fflktswiha esraaavdkl rrmntslwps
      481 dsylnaseva ailenfpepr edgavlrywl dalsarrall grqwdvadaf ypgdlqppra
      541 lfryhyylne lavslhalrs plfvegvgla vnmgglgahy avalarafdp rgvlldgrgs
      601 tsglwwgkss yrdyerrtac pataasgaeg gvqafegvaa ieiacgafna srsiadqgtr
      661 thqrrriflg lsedqaffva fcqascargp serkqlsctl plknfrefat aftcpvaspm
      721 npalrcgffe asdlraarkn ggesedagks astnasanvg adgavtswtr pparrsgsdd
      781 qdpltlattg laghmvrqpp vl
//
LOCUS       XP_077508993             469 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme-like 1 [Amblyomma americanum].
ACCESSION   XP_077508993
VERSION     XP_077508993.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652867.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..469
                     /product="endothelin-converting enzyme-like 1"
                     /calculated_mol_wt=52657
     Region          17..>449
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..469
                     /gene="LOC144120454"
                     /coded_by="XM_077652867.1:1..1410"
                     /db_xref="GeneID:144120454"
ORIGIN      
        1 mahfrrlkds lnrstapcgd fyrfacgawh praaksetvf rdllavaqqd airyleavnd
       61 asnkmvlptg kvlvpdsrpv lrinraerac qvclhsgsst rnaalltefl anrslswper
      121 ptapaqpldv lldldinwnl glwfwvrvsp ipgrnqrqvr vgsgivssdw kraldtmvtr
      181 gvykarvrrf hdalrarsfa amsdkeveel rryesrivga lsgsvsggqn elafplkqmq
      241 nlatprlata qwvtllnwqf nkalrvtean rviatnvkll qavdyllssl paevvlhqla
      301 wslvqmlgwm aasalpgrpt ayemgdmrsa dcfwathraf gvtllsgyin ttyplhwrss
      361 vdallmtitq tainfflkts widaesraaa vdklrrmnts lwpsdsylna sevaailenf
      421 pelredgavl rywldalsar rallgrqwdv adafspgdlq ppralfryh
//
LOCUS       XP_077509005             549 aa            linear   INV 25-NOV-2025
DEFINITION  neprilysin-1-like [Amblyomma americanum].
ACCESSION   XP_077509005
VERSION     XP_077509005.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652879.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..549
                     /product="neprilysin-1-like"
                     /calculated_mol_wt=61440
     Region          93..>525
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..549
                     /gene="LOC144120463"
                     /coded_by="XM_077652879.1:1..1650"
                     /db_xref="GeneID:144120463"
ORIGIN      
        1 magegceaps ttsggldrrq ravalghlls sllgvaltls aitiglsvlh hqrgsegsgv
       61 fgmgrtswnt cnsklcmahf rrlkdslnrs tapcgdfyrf acgawhpraa ksetvfrdll
      121 avaqqdairy leavndasnk mvlptgkvll pdsrpvlrin raerayqvcl hsgsstkdaa
      181 llteflagrs lswperttap aqpldvlldq dinwnlglwf svrvspipgr nqrqvrvgfg
      241 ivsydwkral dtmvtrgvyk arvrrfhdal rarrfaamsd kemeelrrye stivgalcgs
      301 vsggqnelaf plkqmqnlat prlataqwdt llnwqlnkar rvteadrvia tnvkllqavd
      361 yllsslpaev vlhqlawsvv qmigwmvdsa lpgrptayem edmrsadcfw vthrafgvth
      421 fsgyinttyp lhwrssvdai lmtitqtain fflktswiha esraaavdkl rrmntslwps
      481 dsylnaseva ailenfpepr edcavlrywl dalsarrall grqwdvaddf ypgdlqpppa
      541 lfryhyyln
//
LOCUS       XP_077509017             121 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120465 [Amblyomma americanum].
ACCESSION   XP_077509017
VERSION     XP_077509017.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652891.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 34% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..121
                     /product="uncharacterized protein LOC144120465"
                     /calculated_mol_wt=13698
     CDS             1..121
                     /gene="LOC144120465"
                     /coded_by="XM_077652891.1:1..366"
                     /db_xref="GeneID:144120465"
ORIGIN      
        1 mdkllspclr srevaghasr llpqlrahde eyyrdflrmp prtfdtlldl vrssitvedt
       61 rfrsaipaad flvitirhak qvhdklthhf vtevavpwqn amvtgdhkkl kvspstslpd
      121 a
//
LOCUS       XP_077509029             148 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120474 [Amblyomma americanum].
ACCESSION   XP_077509029
VERSION     XP_077509029.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652903.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="uncharacterized protein LOC144120474"
                     /calculated_mol_wt=17062
     Region          10..100
                     /region_name="MADF_DNA_bdg"
                     /note="Alcohol dehydrogenase transcription factor
                     Myb/SANT-like; pfam10545"
                     /db_xref="CDD:463144"
     CDS             1..148
                     /gene="LOC144120474"
                     /coded_by="XM_077652903.1:1..447"
                     /db_xref="GeneID:144120474"
ORIGIN      
        1 mprelfnell iaevakrpll wdvkdnkfrn rawkealwee vrdalrknya tvtteevmnr
       61 wknlkdtfrk kikneadtks sgaeagikss wpymqqmefl rdtvetrrsl cnlaptdlsv
      121 gsaelhmasp vsnekepevp ldelevpv
//
LOCUS       XP_077509042             189 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120481 [Amblyomma americanum].
ACCESSION   XP_077509042
VERSION     XP_077509042.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652916.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..189
                     /product="uncharacterized protein LOC144120481"
                     /calculated_mol_wt=21222
     Region          <55..133
                     /region_name="DDE_3"
                     /note="DDE superfamily endonuclease; cl46863"
                     /db_xref="CDD:481203"
     CDS             1..189
                     /gene="LOC144120481"
                     /coded_by="XM_077652916.1:1..570"
                     /db_xref="GeneID:144120481"
ORIGIN      
        1 mhpalqslrl viwaylsgry dpryvhdvka ssrtsvnvwe vitkdglgpl hridgrlrst
       61 tyvaiidlql lphvlygpyr dgdfllqhyl spihlahvvt ahlenlgert ldwpptgadm
      121 niienvwgil kanrskmgih tatpvelcva tsrqweelka dsdlvpalyd slprrtsaav
      181 gndsdftry
//
LOCUS       XP_077509078             362 aa            linear   INV 25-NOV-2025
DEFINITION  adrenocorticotropic hormone receptor-like [Amblyomma americanum].
ACCESSION   XP_077509078
VERSION     XP_077509078.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652952.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..362
                     /product="adrenocorticotropic hormone receptor-like"
                     /calculated_mol_wt=39998
     Region          25..298
                     /region_name="7tm_classA_rhodopsin-like"
                     /note="rhodopsin receptor-like class A family of the
                     seven-transmembrane G protein-coupled receptor
                     superfamily; cd00637"
                     /db_xref="CDD:410626"
     Region          25..47
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410626"
     Region          54..89
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410626"
     Site            order(85,88..89,97..102,104..105,108,153,155..159,181,
                     184..186,188..190,192,247,250..251,253..254,263,273..274,
                     276..278,281,284..285)
                     /site_type="other"
                     /note="putative ligand binding pocket [chemical binding]"
                     /db_xref="CDD:410626"
     Region          97..127
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410626"
     Region          140..159
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410626"
     Region          181..205
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410626"
     Region          227..263
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410626"
     Region          274..298
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410626"
     CDS             1..362
                     /gene="LOC144120502"
                     /coded_by="XM_077652952.1:1..1089"
                     /db_xref="GeneID:144120502"
ORIGIN      
        1 mesnssvprg lgedqravya vavpvllvgc slafvfnagv llslrwlrrp msptlcfsls
       61 ltfadaysal llatglvlns ylpvygvslg sqercivlil evfrlsglla safhllalav
      121 nhyvgilrpl hyaamvtrrs selaiavlwa lpvlvflvyf ssvpgqglqs pgcvevgfvr
      181 grafsltlla lfsaplvvmt fiyshifvii rrhraglvsf psarqlhrnv kaayttlwil
      241 gtyligwmpa vafivltctd cafpiydlpm strmtlgilt naliickgli dpfiyaarmf
      301 dvkealqnml rcrprrtksv crrsflvtkt dtltsslrli qlrresfiva kprpeicrcs
      361 ra
//
LOCUS       XP_077509089             155 aa            linear   INV 25-NOV-2025
DEFINITION  WD repeat-containing protein 47-like [Amblyomma americanum].
ACCESSION   XP_077509089
VERSION     XP_077509089.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652963.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..155
                     /product="WD repeat-containing protein 47-like"
                     /calculated_mol_wt=17090
     Region          75..124
                     /region_name="CTLH"
                     /note="C-terminal to LisH motif; smart00668"
                     /db_xref="CDD:128914"
     CDS             1..155
                     /gene="LOC144120509"
                     /coded_by="XM_077652963.1:145..612"
                     /db_xref="GeneID:144120509"
ORIGIN      
        1 mqcagcrgsg snhrcvvflf geqpnptete maahlcleea evvrlvlefl anwelsisql
       61 vleresgvin daisddllfl rqlildgqwd nvldfvqple gmgafdskrf kfsvlkhkfl
      121 ellcirseag lvqsaelgve lggrpwspga mrpvs
//
LOCUS       XP_077509115             320 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120519 [Amblyomma americanum].
ACCESSION   XP_077509115
VERSION     XP_077509115.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077652989.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..320
                     /product="uncharacterized protein LOC144120519"
                     /calculated_mol_wt=34313
     Region          <198..>235
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     CDS             1..320
                     /gene="LOC144120519"
                     /coded_by="XM_077652989.1:1..963"
                     /db_xref="GeneID:144120519"
ORIGIN      
        1 mtgsvgaata amsgrgnrff etdneyevil pslptgrivf ntvflhadvr arpygvedfr
       61 dtlahlkllp dvtalgayrm shvlavafds ahavkkmlaa gemqvkerrc liidpanqdv
      121 rmkihwllpg vpdeelraaf vpygkvtevk aerwrvqgit dkgsttrlgs lslkagvkld
      181 dlphqvsiag elalvvvpgr aplclrcrgk ghvrreckip rcgacrrsgh aesecartya
      241 svtgrssnea aselmmdeld aeeaatgktg kggnvletlv phstsvaegs grlepapsvp
      301 qddlpdekaa vledaaarpn
//
LOCUS       XP_077509130             111 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120524 [Amblyomma americanum].
ACCESSION   XP_077509130
VERSION     XP_077509130.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653004.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..111
                     /product="uncharacterized protein LOC144120524"
                     /calculated_mol_wt=12638
     Region          <5..105
                     /region_name="P2RX7_C"
                     /note="P2X purinoreceptor 7 intracellular domain;
                     pfam20478"
                     /db_xref="CDD:466627"
     CDS             1..111
                     /gene="LOC144120524"
                     /coded_by="XM_077653004.1:21..356"
                     /db_xref="GeneID:144120524"
ORIGIN      
        1 mpialestcc reisaatvkq psgcitehph fytlcldevv ltvalqmlld hglhvehtrr
       61 yryisysqlg hwlwgrlgrh hrkvlpacav hqirerlpte gytgfqyaqg l
//
LOCUS       XP_077509146             320 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120525 [Amblyomma americanum].
ACCESSION   XP_077509146
VERSION     XP_077509146.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653020.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..320
                     /product="uncharacterized protein LOC144120525"
                     /calculated_mol_wt=34313
     Region          <198..>235
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     CDS             1..320
                     /gene="LOC144120525"
                     /coded_by="XM_077653020.1:1..963"
                     /db_xref="GeneID:144120525"
ORIGIN      
        1 mtgsvgaata amsgrgnrff etdneyevil pslptgrivf ntvflhadvr arpygvedfr
       61 dtlahlkllp dvtalgayrm shvlavafds ahavkkmlaa gemqvkerrc liidpanqdv
      121 rmkihwllpg vpdeelraaf vpygkvtevk aerwrvqgit dkgsttrlgs lslkagvkld
      181 dlphqvsiag elalvvvpgr aplclrcrgk ghvrreckip rcgacrrsgh aesecartya
      241 svtgrssnea aselmmdeld aeeaatgktg kggnvletlv phstsvaegs grlepapsvp
      301 qddlpdekaa vledaaarpn
//
LOCUS       XP_077509162             111 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120526 [Amblyomma americanum].
ACCESSION   XP_077509162
VERSION     XP_077509162.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653036.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..111
                     /product="uncharacterized protein LOC144120526"
                     /calculated_mol_wt=12638
     Region          <5..105
                     /region_name="P2RX7_C"
                     /note="P2X purinoreceptor 7 intracellular domain;
                     pfam20478"
                     /db_xref="CDD:466627"
     CDS             1..111
                     /gene="LOC144120526"
                     /coded_by="XM_077653036.1:21..356"
                     /db_xref="GeneID:144120526"
ORIGIN      
        1 mpialestcc reisaatvkq psgcitehph fytlcldevv ltvalqmlld hglhvehtrr
       61 yryisysqlg hwlwgrlgrh hrkvlpacav hqirerlpte gytgfqyaqg l
//
LOCUS       XP_077509219             225 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120555, partial [Amblyomma
            americanum].
ACCESSION   XP_077509219
VERSION     XP_077509219.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653093.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 26% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         <1..225
                     /product="uncharacterized protein LOC144120555"
     CDS             1..225
                     /gene="LOC144120555"
                     /coded_by="XM_077653093.1:<1..678"
                     /db_xref="GeneID:144120555"
ORIGIN      
        1 llsdnctykh pegiaawide trewpsicsp dvvfylldsk awdlqamrny rslesynyrq
       61 sgyegkilfh kisadaryvk aavcpsqsvn aaprnawacv krtgevetag cscmagngkt
      121 cshvgvllwk vqigvseglt gtsctdkaaf wnsgtkrnve pamlehidfk lqkgtvdgss
      181 rkrprprfvp mtqeeiqkmh eeppyaelft fpgtvlyesl hapdr
//
LOCUS       XP_077509231             962 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120563 [Amblyomma americanum].
ACCESSION   XP_077509231
VERSION     XP_077509231.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653105.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..962
                     /product="uncharacterized protein LOC144120563"
                     /calculated_mol_wt=110070
     Region          207..296
                     /region_name="SCAN"
                     /note="SCAN domain; pfam02023"
                     /db_xref="CDD:460417"
     Region          364..379
                     /region_name="zf-CCHC"
                     /note="Zinc knuckle; pfam00098"
                     /db_xref="CDD:395050"
     Region          646..702
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          722..817
                     /region_name="rve"
                     /note="Integrase core domain; pfam00665"
                     /db_xref="CDD:459897"
     CDS             1..962
                     /gene="LOC144120563"
                     /coded_by="XM_077653105.1:873..3761"
                     /db_xref="GeneID:144120563"
ORIGIN      
        1 mdlmkllrsd llslceelgv dveekmkksh ictllletan eetvsdtwel lkaerkkked
       61 erkkeederk keedererkr eeterqrlva ereerelrrl qlandqkkle cessrgmtpe
      121 rvsqaesfpm drlmqpykig edlgyylvnf ertcekrgya ketwpqkllm vlpceaativ
      181 arldakdadd yekvkasllr kyrlsaeefr qrfrgaskrs sesyaefafs lkanltewlk
      241 gaeaydtkdk vvecvgleqf ynsipeavkl wvqdrenvst vekaaelade yatrrklsse
      301 ssesekkafg lrkrfipknr sqfrnretve gikqapekte drakqagaqk altkefeark
      361 pfrcfncqet ghiaarctkt rlafsyands denlellrpy ihelqvngkl crvlrdsgat
      421 mdivhpsyvk eedftgevaw irqvlenqsv clpmarvvis gpfgelatea avssavslqy
      481 pylfsnksnq llcdrgqkla egtvkaltrs ksrelasklr ysqeseatge regipeiaep
      541 skenavrahe qqqepvtegs pidaslqeac spdsmllpts lcmdrllrvd rdsliteqer
      601 dpslsklhft akegiarrnv tlhhkgglly rhyrdkkgkt ldqlvvpeky rsdllglchg
      661 nswsghlgvn ktkerllgey ywpgcfrdae ryvqscdacq rtgkpgerwk aplklvplis
      721 epfrrlvidt vgplprtksg ykyvltmicp atkfpeavpl kelssaeivd gllsvfarig
      781 fpaeiqadqg tvftsalttt flqrcgvrli hssvyhpqsn svekwhsvlk rvlrascyeh
      841 kddwesclpa tlfalrtvph eatgftpael vygralrspl rmlremwegt gesqsvveyv
      901 lqllnrlsat relvdrnlkt aqdtakkyyd knarlrtfkt gdhldvrdel dtverynqic
      961 lm
//
LOCUS       XP_077509243             259 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077509243
VERSION     XP_077509243.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653117.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 20% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..259
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=28975
     Region          <48..208
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; cl46765"
                     /db_xref="CDD:481105"
     CDS             1..259
                     /gene="LOC144120573"
                     /coded_by="XM_077653117.1:1..780"
                     /db_xref="GeneID:144120573"
ORIGIN      
        1 mtlgalaals llgciisawt vsdsevmpfm mvpdfkssvl mktmnayyvr psyhalcyfs
       61 gcitflimdd frrlkistgt qlvgwcvats cclcvvfmka pwyhrpnpas qfvkilatvs
      121 drllwslfla wttlacstgr gglfarllsw nvfvplsklt fgvylihfpf itlmlhasre
      181 riyvshftql tlffgvltws lllaylaflm cegpsttlyk safqifirrg ktaqrnrghq
      241 agtngasaek petshvhsq
//
LOCUS       XP_077509259             760 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077509259
VERSION     XP_077509259.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653133.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..760
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=84944
     Region          76..202
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          295..695
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..760
                     /gene="LOC144120576"
                     /coded_by="XM_077653133.1:1..2283"
                     /db_xref="GeneID:144120576"
ORIGIN      
        1 mlvpslvflf lvcaprlgrc leseggqegs ssgggppedv fdgyfddmrr kigeviartp
       61 pslmrklqsa dvsarcslgl lkllrgvrnl epwamklfda sakyptglfh gtrvdlgafd
      121 eclettvaga sghitsrgqy cnlrfyikna tawqkkmgpf lsvlhptmry fkdyffleev
      181 piirlgicfl edctqrdlqa lvdsvkpsil dievsnclta erepwsttqk siviflgflv
      241 itvigctcvd lfmqskrklg gkrsalvplv tafsatantq alvkaadkds dlyvlqflhg
      301 lrlfslayiv lghcyqlmsd twsrllnmlv svdewsnmmv vagfscvdtf fflsgfflta
      361 tvmrqkrngp mvfvigllrr lvrtciplff iimclcvlpl ivagpdmdsf ysklykdvag
      421 hwwqlllqir nyfelnqdtv lghtwylaad fqlflvsllv llllksrktl algafaglcl
      481 lgcaiatwtv snpeitpfvv fpaktpqmtm ktaneyyvrp fyhavcyfsg cmaflviddf
      541 rkrklskamq fvgwcaamgc svscvfmkla wyttatstse agdlfgaffd rilwslflaw
      601 vtlacstgqg gvlgrflsmk vfvpfsrlsf gvylihypfi eimlhasrer llwshfsqvt
      661 lffgvlcwsf ilaylayllc eaptaaldkl afqrltgkgn arsqvpqpds gdvvpekrae
      721 ggrvvstlts gknngyglpd pwngavrgls wnsqwmpnrl
//
LOCUS       XP_077509273             419 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-(1,3)-fucosyltransferase C-like [Amblyomma americanum].
ACCESSION   XP_077509273
VERSION     XP_077509273.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653147.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..419
                     /product="alpha-(1,3)-fucosyltransferase C-like"
                     /calculated_mol_wt=47061
     Region          <131..209
                     /region_name="Glyco_tran_10_N"
                     /note="Fucosyltransferase, N-terminal; pfam17039"
                     /db_xref="CDD:465336"
     Region          <300..394
                     /region_name="Glyco_transf_10"
                     /note="Glycosyltransferase family 10 (fucosyltransferase)
                     C-term; pfam00852"
                     /db_xref="CDD:459962"
     CDS             1..419
                     /gene="LOC144120578"
                     /coded_by="XM_077653147.1:1..1260"
                     /db_xref="GeneID:144120578"
ORIGIN      
        1 mccrsaialf vvlgavvail llrafqtpsr eekygseyrp ltlapigsev eshlrrgtst
       61 vssqlddast glsgvsmlgh lqprillwae pgerrpvegv hlpaegeial rycsfvlqna
      121 lpdavvatfp pdcyvtrdys qlnqsdaivf dafavhgtip pifrvssqvw vfwthhgsap
      181 tadlgsmttd afnwtmgrrm dadvaipygr wtrreraapr dfakkrhakn gtalfftsdc
      241 drkedmqvlt evmdalqgsa irgcgasdcg rfqlcvreha eqfhflfvan ssgcyehple
      301 aiydafrydl ipvyfgqktl vglpehsfvd afamvratdi idylsallkd wdlycaffkw
      361 keeyvlssrd dlcylcdalk knlqrkttdv vawwkksnlc plnhrisqah sqpheayvh
//
LOCUS       XP_077509305             384 aa            linear   INV 25-NOV-2025
DEFINITION  lysine-specific demethylase 8-like [Amblyomma americanum].
ACCESSION   XP_077509305
VERSION     XP_077509305.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653179.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..384
                     /product="lysine-specific demethylase 8-like"
                     /calculated_mol_wt=43703
     Region          191..384
                     /region_name="Cupin_8"
                     /note="Cupin-like domain; pfam13621"
                     /db_xref="CDD:463936"
     CDS             1..384
                     /gene="LOC144120603"
                     /coded_by="XM_077653179.1:324..1478"
                     /db_xref="GeneID:144120603"
ORIGIN      
        1 mcdccrwpis tdftlvftmt slaekhlgcl vtfvtddivd gdepvvfllr kcvdfvnhah
       61 acedmtalqe nfetaraird msweminigh wkdvplgwrr mysygsllka lcelgqakpm
      121 edilntilga pvmenvlakl ativhkelpe lissqvlkkv sifvlnflay dsttggakll
      181 lvsfffssir hlvtvaggrt vpvelgsryt deewsqklmt lasfvrtyil keegpdvgvg
      241 ymaqhqlfhq ipelrddici ptycclsekd eepdinlwfg pegtvsplhh dpknnllaqv
      301 fgekyirlyg kaqtpflypy eeqllentsq vdiedpdfdk fplfekaeyt ecvlkageml
      361 fippkcwhfv rslspslsis fwwe
//
LOCUS       XP_077509319             427 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120609 [Amblyomma americanum].
ACCESSION   XP_077509319
VERSION     XP_077509319.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653193.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..427
                     /product="uncharacterized protein LOC144120609"
                     /calculated_mol_wt=46114
     Region          378..412
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..427
                     /gene="LOC144120609"
                     /coded_by="XM_077653193.1:1..1284"
                     /db_xref="GeneID:144120609"
ORIGIN      
        1 maaqgsreqs qysrrssrsq rrgrlprala aedrlseara srelpppppp pppqqhpwad
       61 srqsagrsta sslisrlgkh ssrppssmld eeaamrrsat svagaasgmt pvrltryygp
      121 prlgrerqee eaaefgphvd gsrtwevgge mpgrrldrap laadaggsrv srrsasrqar
      181 tvaecvapas tpprparskd agdrphrtle mtfamgafvc vatlvvafvv rawmpyears
      241 dpfcrsdacl ahvrlieari drgvdpcvdf dayacsrwkp asefyghasa ltnvlldnwr
      301 rwalngaara vspaevaver eqpdeddemr eaaaaeaaav avapaapaqp cpvgmrpaav
      361 lthhlaagga aaqecssdpe elfclslaad lkrllprerh marmkmlqtl hdlefgenig
      421 eaalgep
//
LOCUS       XP_077509345             308 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120619 [Amblyomma americanum].
ACCESSION   XP_077509345
VERSION     XP_077509345.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653219.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 16% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..308
                     /product="uncharacterized protein LOC144120619"
                     /calculated_mol_wt=35103
     Region          93..255
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..308
                     /gene="LOC144120619"
                     /coded_by="XM_077653219.1:1..927"
                     /db_xref="GeneID:144120619"
ORIGIN      
        1 mqrehtnmre aisvekrigi glfklcsaae drvvaivfgv grstvntiyr efaetvvdvl
       61 epewarmmap nemnehvsef yamtgfpqam galdgchfpl spphlnaedy rnykgwysvi
      121 llavvdhkyr fryvsagspg rchdshvysq sdlaetverp lfktpvtvin gtavppiilc
      181 dqafaltghl mkpfphngpl qeekrlfnyh lsktrriven afgrmkarfr ytmkrmecei
      241 rhvpviirac ctlnnmcedf garlpqqwld evsqhdrafe qpahttqlev dsgasvraal
      301 vhyfqqlq
//
LOCUS       XP_077509355             239 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120629 [Amblyomma americanum].
ACCESSION   XP_077509355
VERSION     XP_077509355.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653229.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..239
                     /product="uncharacterized protein LOC144120629"
                     /calculated_mol_wt=22908
     CDS             1..239
                     /gene="LOC144120629"
                     /coded_by="XM_077653229.1:1..720"
                     /db_xref="GeneID:144120629"
ORIGIN      
        1 mqglskkgee hvagaifdsl qdmcrmlpss anivllvvat yqlftiaavt alvagsvfae
       61 dvaknkdkkd ekkeveargg ilgagvglgg lggygagvgp glvgeglggp givgtgavgt
      121 pglvgagvag gigggflsgy gssagghlsg yqvgssgqnq gsgafasgas crkvdsfsnn
      181 qgyshssdfs vsdskifgsg qkqgtsgfsn gasghqagfg qsayrksagv gvggaglhg
//
LOCUS       XP_077509372             411 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120633 [Amblyomma americanum].
ACCESSION   XP_077509372
VERSION     XP_077509372.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653246.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 50% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..411
                     /product="uncharacterized protein LOC144120633"
                     /calculated_mol_wt=45189
     Region          <3..53
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          307..>405
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..411
                     /gene="LOC144120633"
                     /coded_by="XM_077653246.1:1..1236"
                     /db_xref="GeneID:144120633"
ORIGIN      
        1 mftlhrfpts palrqqciaa vnrknfdate asrvcsrhfv ggkkteenav pmldmgyekk
       61 smsddkdtdt pleeyeacla pddmveepks devatavvvp dmaeaavldq vliptmaaae
      121 taeemtedkl tpavatpema ddgipdeaap aleqspevrt astqwedqnn ehsycqhglg
      181 tlsnkqqlsa stlgeddcvf ytglgkthfq nlvtavkves rklftvlpye dqllltlmrl
      241 rlgllyghla rifqisvgsv cnifmhvlsv lctimgkvlt wlprsiirns mpksfidngy
      301 dtttcivdcs einlqrpkkl mpraqtyssy kahntvkfli aiapdgyimy vspayggras
      361 dkyitkdckl eddlgpgdev tadrgftltt smiiegvkln vpaftkgkgi v
//
LOCUS       XP_077509383             234 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120641 [Amblyomma americanum].
ACCESSION   XP_077509383
VERSION     XP_077509383.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653257.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 16% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..234
                     /product="uncharacterized protein LOC144120641"
                     /calculated_mol_wt=25976
     Region          79..>153
                     /region_name="SWIM"
                     /note="Uncharacterized protein, contains SWIM-type Zn
                     finger domain [Function unknown]; COG4715"
                     /db_xref="CDD:443750"
     CDS             1..234
                     /gene="LOC144120641"
                     /coded_by="XM_077653257.1:125..829"
                     /db_xref="GeneID:144120641"
ORIGIN      
        1 mgtenearda sasssassss rhvlisetmt ythpddikdw sedmrdwpei rgpyivfylv
       61 qtkvcdllev raykslesyn ylqsgrvgkv lahrvsddiv mlksevtpsq avssrphqvw
      121 lsakstgevl ragcscmagq arvcshvgav lwkvdcavsr gftgrtctda aakcnrgtkr
      181 nveprrldqi nfklkektvd pevqahsfmq rsmqhhgspa dfgkrqhhma amks
//
LOCUS       XP_077509396             342 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120648 [Amblyomma americanum].
ACCESSION   XP_077509396
VERSION     XP_077509396.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653270.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..342
                     /product="uncharacterized protein LOC144120648"
                     /calculated_mol_wt=38378
     Region          194..280
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..342
                     /gene="LOC144120648"
                     /coded_by="XM_077653270.1:1..1029"
                     /db_xref="GeneID:144120648"
ORIGIN      
        1 mlytrrkdgs skscialftc attrsihlel vsnlttecfl lalrrfvarr glpstiysdt
       61 altfkkapkd lqmlwnilrs retqdyttsq qlswkfivek aawwggwwer mvktvketll
      121 kilghqslsf eelatvladv eatinsrpla yasadtteps iltpshlitg krlialpkar
      181 tasipsatat digrrwkyrq timnsfwkrw lkeyilqlrs lhfassspas viavddsviv
      241 hkenaprhml qtvrvvevfk grdgharsct vkmpsgviir rpmatglvtk astifivllt
      301 vstvgyyife wkdappwlka aftgtstnqs vgldtthptq lg
//
LOCUS       XP_077509421             223 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120662 [Amblyomma americanum].
ACCESSION   XP_077509421
VERSION     XP_077509421.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653295.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 11% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..223
                     /product="uncharacterized protein LOC144120662"
                     /calculated_mol_wt=25267
     CDS             1..223
                     /gene="LOC144120662"
                     /coded_by="XM_077653295.1:232..903"
                     /db_xref="GeneID:144120662"
ORIGIN      
        1 maslpkeraq sraqdatrkt rqeknhrdsn ldlpalylak gslfgwisdp gsdtssegsa
       61 gdlalgvetv defpyvkisr aemkkqraml anieqmlkfv aknlteksvv mpkvmlnkfl
      121 evehiksemc kmqeenrrlk eelhrrdefi ranglelsga gaglviqqas epfedtsvlq
      181 eqqqnaclpd ssqrqaqdam lrkmlwnark eeikrkhkqr pae
//
LOCUS       XP_077509450             185 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120686 [Amblyomma americanum].
ACCESSION   XP_077509450
VERSION     XP_077509450.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653324.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..185
                     /product="uncharacterized protein LOC144120686"
                     /calculated_mol_wt=19942
     CDS             1..185
                     /gene="LOC144120686"
                     /coded_by="XM_077653324.1:1..558"
                     /db_xref="GeneID:144120686"
ORIGIN      
        1 miatrniqaa vdgpfsllmc itmmrwpfpg sllqamvvli laqaaharcs vlpptllllp
       61 lhishpengs sgehtaahlq lmvhlngggg pqtadrttef lwqcalpqlr aitkkmhspv
      121 htygevslpe qvqgvlrrgp kfaveprqsa sellamvrqv sslavapevd rcvsdgvyil
      181 akttr
//
LOCUS       XP_077509464             169 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120691 [Amblyomma americanum].
ACCESSION   XP_077509464
VERSION     XP_077509464.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653338.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..169
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..169
                     /product="uncharacterized protein LOC144120691"
                     /calculated_mol_wt=18532
     CDS             1..169
                     /gene="LOC144120691"
                     /coded_by="XM_077653338.1:1..510"
                     /db_xref="GeneID:144120691"
ORIGIN      
        1 miatrniqaa vdgpfsllmc itimrwpfpg sllqamfvli laqaaharrs vlpptlrllp
       61 lhishpengs sgehtaahlq lmvhlngggg pqtadrttef lwqcalpqlr aitkkmhspv
      121 htygevslpe qvqgvlrrgp kfaveprqsa sellametyr rplmgfshc
//
LOCUS       XP_077509487             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120706 [Amblyomma americanum].
ACCESSION   XP_077509487
VERSION     XP_077509487.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653361.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 25% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144120706"
                     /calculated_mol_wt=17527
     Region          1..>60
                     /region_name="SanA"
                     /note="Uncharacterized periplasmic protein SanA, affects
                     membrane permeability for vancomycin [Cell
                     wall/membrane/envelope biogenesis]; COG2949"
                     /db_xref="CDD:442192"
     CDS             1..158
                     /gene="LOC144120706"
                     /coded_by="XM_077653361.1:342..818"
                     /db_xref="GeneID:144120706"
ORIGIN      
        1 mrkalstckk tlvaiwaahs ylapvlslqi vsmqtspcvs elisdlpptp mvvvlgtsif
       61 dcdctcivtl dgakcfegls flaavetlya ayfclnmkyn qevqatlefi qrqlvgmnpk
      121 egsrqlnrri tsvhpkvhsa lqqldklkkk wqdcmsri
//
LOCUS       XP_077509498             887 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-4-like [Amblyomma americanum].
ACCESSION   XP_077509498
VERSION     XP_077509498.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653372.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..887
                     /product="protein argonaute-4-like"
                     /calculated_mol_wt=100933
     Region          122..191
                     /region_name="ArgoN"
                     /note="N-terminal domain of argonaute; pfam16486"
                     /db_xref="CDD:465134"
     Region          201..252
                     /region_name="ArgoL1"
                     /note="Argonaute linker 1 domain; pfam08699"
                     /db_xref="CDD:462567"
     Region          258..388
                     /region_name="PAZ"
                     /note="PAZ domain; pfam02170"
                     /db_xref="CDD:460472"
     Region          415..844
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(547,551,563..566,569,578,581,585,589)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(616,618,694,834)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..887
                     /gene="LOC144120713"
                     /coded_by="XM_077653372.1:1..2664"
                     /db_xref="GeneID:144120713"
ORIGIN      
        1 maphsrqqra clgtfaalds cqtashglei ertlpshfpr rptqgklgrp iqltanhfsv
       61 eipsgnvfhy dveicsesrk etnkvpekrk yrcistkinr lviellvkky rvdlsgcmpa
      121 fdgrknlytr relnfrertf tvdfeedqri qkfivkiqya atvnldalqa vfdrrvshvp
      181 qevlqavdiv lrhgpsiklt pvgrsffrpp apgeyntlgg grevwfgyyt svrpaqwkpm
      241 lnvdisataf yeplpvtdfm cklfsdcrre msagdfknlr dfqnvrlnke lkglrvkvth
      301 lpyprkykvi rvtkepakkl yfemedgtrc svadyfqnry drlkypnlpc iqtgsenhpv
      361 ylplevcviv egqhckkkld enqtsemikr taqppakrfn eirqsvrdlv sssdqclref
      421 gikisteptq lkgrvldpps lvfennnigk pregtwelrg rhfyksatlt rwtllnlsqf
      481 aqrdsldnfv kmlirvgqel gmsieqpldv tttdtnrkpv rnilleeqrk tanlemviiv
      541 lakntsyadi kqvaeteigl rtqcvmdnnv ikkcnaalit nlcqkmnakl gginnsllpk
      601 ekpkifqkpv iiigadvthp apgdklrpsi aacvgsldai pykfhasvrv qmadsaatsr
      661 leiikdlkdm mkdtlkafyh atrhkperii fyrdgvsegq fmevrnrevs airlacqels
      721 pnetyepalt fivvqkrhht rfmpandrdg vgkcrnvppg ttvdsvvthp ldfdfylcsh
      781 fgiqgtsrps hyyilwddss ftaddvqyls yylchtyarc trsvsipapv yyahlaayra
      841 kkhlmskvdv sssssdssgg sadavstrqy aeavkvlesl ktgmyfv
//
LOCUS       XP_077509519             520 aa            linear   INV 25-NOV-2025
DEFINITION  sodium-coupled monocarboxylate transporter 1-like [Amblyomma
            americanum].
ACCESSION   XP_077509519
VERSION     XP_077509519.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653393.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 15% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..520
                     /product="sodium-coupled monocarboxylate transporter
                     1-like"
                     /calculated_mol_wt=56491
     Region          9..501
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     Site            order(68,71,356,359..360)
                     /site_type="other"
                     /note="Na binding site [ion binding]"
                     /db_xref="CDD:271356"
     CDS             1..520
                     /gene="LOC144120729"
                     /coded_by="XM_077653393.1:66..1628"
                     /db_xref="GeneID:144120729"
ORIGIN      
        1 maapplyvad yltvgsisal gigvglaatf fgrrpkaeaa tsqdndqeea flgsrrlyai
       61 plavsmfasa vtstsvvsft ahfyvyglhv vwgavskamv apimahafvp vvyglrltsv
      121 fqylrmrynd glgvtacviy fflsvifggl sictcslals tilganfimw nlviglagta
      181 ytvlgkirgv vwadtvqglv mfaspvavva kvvydcynee dplrrrpiwd fdfrrfifrt
      241 dfdlsndeti waatlgllpl ffvrygfdqi vaqrylsars lrdaqsvayr gcalgaifll
      301 vvgvmamsii ywyrdcdprl agaiprydqi vpyyinenls ilpgmrgvfl agvvgasmst
      361 vssiinshsa vfyvdvvtpy fskakkrpsy visiiaiasg vmmtgfatll pymgtaarvt
      421 mvicnsisgp lcglfllall fpwsntkgaa sstlvslall lwhsvgrsfs giepprmdvs
      481 idgclanvta vstsalrhvs pkqlrertvq hlqdtatkql
//
LOCUS       XP_077509567             862 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-4-like [Amblyomma americanum].
ACCESSION   XP_077509567
VERSION     XP_077509567.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653441.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..862
                     /product="protein argonaute-4-like"
                     /calculated_mol_wt=98095
     Region          97..166
                     /region_name="ArgoN"
                     /note="N-terminal domain of argonaute; pfam16486"
                     /db_xref="CDD:465134"
     Region          176..227
                     /region_name="ArgoL1"
                     /note="Argonaute linker 1 domain; pfam08699"
                     /db_xref="CDD:462567"
     Region          233..363
                     /region_name="PAZ"
                     /note="PAZ domain; pfam02170"
                     /db_xref="CDD:460472"
     Region          390..819
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(522,526,538..541,544,553,556,560,564)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(591,593,669,809)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..862
                     /gene="LOC144120760"
                     /coded_by="XM_077653441.1:1..2589"
                     /db_xref="GeneID:144120760"
ORIGIN      
        1 maqeiertlp shfprrpaqg klgrpiqlta nhfsveipsg nvfhydveic sesrketnkv
       61 pekrkyrcis tkinrlviel lvkkyrvdls gcmpafdgrk nlytrrelnf rertftvdfe
      121 edqriqkfiv kiqyaatvnl dalqavfdrr vsqvpqevlq avdivlrhgp sikltpigrs
      181 ffkppapgey ntlgggrevw fgyytsvraa qwkpmlnvdi satafyeplp vtdfmcklfs
      241 dcrremsagd fknlrdfqnv rlnkelkglr vkvthlpypr kykvvrvtke pakkiyfkme
      301 dgtlcsvady fqnrydrlky pnlpcietgs tnhpvylple vcvivegqhc kkkldetqts
      361 emikrtaqpp akrfneirqs vrdlvsssdq clrefgikis teptqlkgrv ldppslvfen
      421 nyigkpregt welrgrqfyk satltrwtll nlsqfaqrds ldnfvkmlir vgqelgmsie
      481 qpldvtttda nrkpvrnill eeqrktanle mviivlaknt syadikqvae teiglrtqcv
      541 mdnnvikkcn aalitnlcqk inaklgginn sllpkekpki fqkpviiiga dvthpapgdk
      601 lrpsiaacvg sldaipykfh asvrvqmaas aatsrleiik dlkdmmkdtl kafyhatrhk
      661 periifyrdg vsegqfmevr nrevsairla cqelspnety epaltfivvq krhhtrfmpa
      721 ndrdgvgkcr nmppgttvds vvthpldfdf ylcshfgiqg tsrpshyyil wddssltade
      781 vqylsyylch tyarctrsvs ipapvyyahl aayrakkhlm skvdvsssss dssggsadsv
      841 stsqyveavk vleslktamy fv
//
LOCUS       XP_077509581             865 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-4-like [Amblyomma americanum].
ACCESSION   XP_077509581
VERSION     XP_077509581.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653455.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..865
                     /product="protein argonaute-4-like"
                     /calculated_mol_wt=98564
     Region          100..169
                     /region_name="ArgoN"
                     /note="N-terminal domain of argonaute; pfam16486"
                     /db_xref="CDD:465134"
     Region          179..230
                     /region_name="ArgoL1"
                     /note="Argonaute linker 1 domain; pfam08699"
                     /db_xref="CDD:462567"
     Region          236..366
                     /region_name="PAZ"
                     /note="PAZ domain; pfam02170"
                     /db_xref="CDD:460472"
     Region          393..822
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(525,529,541..544,547,556,559,563,567)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(594,596,672,812)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..865
                     /gene="LOC144120762"
                     /coded_by="XM_077653455.1:1..2598"
                     /db_xref="GeneID:144120762"
ORIGIN      
        1 mipdqmeier alpshfprrp aqgklgrpiq ltanhfsvei psgnvfhydv eicsesrket
       61 nkvpekrkyr cistkinrlv iellvkkyrv dlsgcmpafd grknlytrre lnfrertftv
      121 dfeedqriqk fivkiqyaat vnldalqavf drrvsqvpqe vlqavdivlr hgpsikltpi
      181 grsffkppap geyntlgggr evwfgyytsv rtaqwkpmln vdisatafye plpvtdfmck
      241 lfsdcrrems agdfqnlrdf qnvrlnkelk glrvkvthlp yprkykvvrv tkepakkiyf
      301 kmedgtrcsv adyfqnrydr lkypnlpcie tgsenhpvyl plevcviveg qhckkkldet
      361 qtsemikrta qppakrfnei rqsvrdlvss sdqclrefgi kisteptqlk grvldppslv
      421 fennnigkpr egtwelrgrh fyksatltrw tllnlsqfaq rdsldnfvkm lirvgqelgm
      481 sieqpldvtt tdanrkpvrn illeeqrkts nlemviivla kntsyadikq vaeteiglrt
      541 qcvmdnnvik kcnaalitnl cqkinaklgg innsllpkek pkifqkpvii igadvthpap
      601 gdklrpsiaa cvgsldaipy kfhasvrvqm adsaatsrle iikdlkdmmk dtlkafyhat
      661 rhkperiify rdgvsegqfm evrnrevsai rlacqelspn etyepaltfi vvqkrhhtrf
      721 mpandrdgvg kcrnvppgtt vdsvvthpld fdfylcshfg iqgtsrpshy yilwddstft
      781 addvqnlsyy lchtyarctr svsipapvyy ahlaayrakk hlmskvdvss sssdssggsa
      841 davstrqyve avkvleslkt gmyfv
//
LOCUS       XP_077509593             107 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120771 [Amblyomma americanum].
ACCESSION   XP_077509593
VERSION     XP_077509593.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653467.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 14% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..107
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..107
                     /product="uncharacterized protein LOC144120771"
                     /calculated_mol_wt=10606
     CDS             1..107
                     /gene="LOC144120771"
                     /coded_by="XM_077653467.1:1..324"
                     /db_xref="GeneID:144120771"
ORIGIN      
        1 metaprsqrr scgtgrptgr spgapsalsf aqavqqrvvr aappqilsss aagaalitra
       61 egtngngaht vaptegvptt stsltaapvq arngvgntks qndelsi
//
LOCUS       XP_077509628             745 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme homolog [Amblyomma americanum].
ACCESSION   XP_077509628
VERSION     XP_077509628.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653502.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..745
                     /product="endothelin-converting enzyme homolog"
                     /calculated_mol_wt=84964
     Region          147..612
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     Region          563..739
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..745
                     /gene="LOC144120793"
                     /coded_by="XM_077653502.1:13..2250"
                     /db_xref="GeneID:144120793"
ORIGIN      
        1 meargfheil yttprlrpnn pmgspltvty lmgvlvflal mtlfafvfsy vvykegadsl
       61 vgsgingsrr epiiyrvcnd tycrnaalyy mgrlrapldm cdqvatslcr atagrnassi
      121 spvrmvkghl hlhgididam lkekyfpvgq lkrflhmcik strksgindf vaimrrfemh
      181 qwpvtpktan mtrllaaiat qlgycplvti slgrgyesrg pvirldkptl llrkvgsqtd
      241 mtmaatllft lakylrdfcd rfaiclqrge aehivttiik feqllsfymr apgkqtrmkl
      301 rdmktnkihw ptllaavlgk hfnataetdv viksphylng ldevleksse svvalyvgyt
      361 ifswfapliv nldastntil spsteqnrqw akfqclllse gvfgfaylha fsslpafdsa
      421 qravinatkm iiktlqeftl ltpwfrkeda amvhkklgsl eviafvpdva kirrftqeyy
      481 ndvpalnmed gflrnlfkmq kfvtersldl aiganaeayt wpvsslrkrc lldmqankly
      541 ipaihtilvd qstrtvkflq mpvlayyvlh allpmffqag svfdergayn ewwnfenees
      601 faraedclvq qylessrvda srdeakgmaa qvmkqnfiia psfrafessl easgysmlnf
      661 rlhpnflads kqvfyaahmt alcdetdtaq nsfiyrmfne dlfdffrqnv pfynfpdyed
      721 qvsdcepaqa nelsrirhcq iwnty
//
LOCUS       XP_077509838             815 aa            linear   INV 25-NOV-2025
DEFINITION  O-acyltransferase like protein-like [Amblyomma americanum].
ACCESSION   XP_077509838
VERSION     XP_077509838.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653712.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..815
                     /product="O-acyltransferase like protein-like"
                     /calculated_mol_wt=90489
     Region          95..224
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          325..710
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..815
                     /gene="LOC144120911"
                     /coded_by="XM_077653712.1:1..2448"
                     /db_xref="GeneID:144120911"
ORIGIN      
        1 mtmrprtptr cvivllvlas aayasaqhds dsatlvsttn atttatttmr tttakpdllk
       61 ekfeamsekv trlvvrelyp iiseliydtr lstgclgslm klgpalrnfd iwavqmvdam
      121 grphagtmqg riaaygaydq clavrhdegl fqgkycmlhl eldgsefsps lrvlvnrfve
      181 hyglqyvgnl srqaeagtpl wvplykvgac vpstcqkedl qiiidhftkd lgsqwkvkwc
      241 tveepvkldr rqsvilcifg vwisfilfgt gydiyrtvls vgdneetgke assgylsrav
      301 tsfslrkafk klmdmpnwgd ysndlgfvhg mrvfsatwvi lghthlirdi hassdsigfl
      361 rriredfsft vqlnafmgve tflvitgfls gylvmkngrl kmspilviiv alfrryirmi
      421 vpmaavmgsi yllpavvdgp mlrehwrgfe vpcdknwwrl flmahnyvqf sdlcvphywy
      481 iavdyqlaiv stiilaavmp kwpklslwlm gaiaavasla taiqtyvmdt lpygliittd
      541 ikrigdmhse iyikpyahag plfvgvifgc lavrrhrmsr lvqgaawala atvslaallg
      601 vrtwsvgrqp erlesafygg lhrvswglgv swvmyacatg rggfvnkila wpvmyplgrl
      661 sfavylvhle vlaattvlgr elvsqqpflh aqmylamtmm sygfaiivyl lfecpvagld
      721 ntafskvmpk qgnqsasktn giaheiksfh tfhcngtstn melssaphqi ingfpdltkd
      781 vgngrrlngy ansvcesdan dddpqgtaaa vsvkl
//
LOCUS       XP_077509853             446 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144120914 [Amblyomma americanum].
ACCESSION   XP_077509853
VERSION     XP_077509853.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653727.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 50% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..446
                     /product="uncharacterized protein LOC144120914"
                     /calculated_mol_wt=49661
     Region          116..285
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     CDS             1..446
                     /gene="LOC144120914"
                     /coded_by="XM_077653727.1:1..1341"
                     /db_xref="GeneID:144120914"
ORIGIN      
        1 mvsmkpsmsg llllfavias qciciraqld eytagtvmsi mnattspast qststtiklk
       61 ttmtsttttt stttaepdrl qvafrelsek mtrivtrefy pvvsdliydp nlssscigsl
      121 mkigtalrtl diwavqsapn ntrhlvahvt fsaqlgllfa apahhsergm qgyvtlidam
      181 grppagmmqg rlsgygaydq clavrhdegl fqgkycmvhl gyngsqmtps lrrvvrkfak
      241 hlgfesagnl trmvnnkgft giaqlykfgv cvpshceped lqvimdhltk dfnlqlkakw
      301 caidepvkln qrqtlilcif gvwvsfilfg taydfykaml wgdeytdsak tfagymsrav
      361 rafslrratk klvqmpnwgd ysnelgfvhg irvlsatwvv lghsymirdp ynnsnfvaff
      421 trlrddflft vqsnsfmave tflvit
//
LOCUS       XP_077509910             410 aa            linear   INV 25-NOV-2025
DEFINITION  cholesterol 7-desaturase nvd 1-like [Amblyomma americanum].
ACCESSION   XP_077509910
VERSION     XP_077509910.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653784.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 15% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..410
                     /product="cholesterol 7-desaturase nvd 1-like"
                     /calculated_mol_wt=46037
     Region          64..408
                     /region_name="PobA"
                     /note="Chlorophyllide a oxygenase/letal leaf spot protein
                     [Coenzyme transport and metabolism]; COG5749"
                     /db_xref="CDD:444459"
     CDS             1..410
                     /gene="LOC144120952"
                     /coded_by="XM_077653784.1:1..1233"
                     /db_xref="GeneID:144120952"
ORIGIN      
        1 mdtslthgga cdgstgwlae gayafilwtv lagalawich rlfmqkrvvr klrnerkyip
       61 ssappvfpng wvplllstdl rvnqvkpvta vgkdlvafrt edgvahvmda ycphlgahlg
      121 vmgrvvgdci ecpfhgwrfq adtgacahvp yvstipaftk vktwtcrety glifvwfhad
      181 geqpewvled iaelssgsfr qmssfettsy ghvqdiaena adtahlncvh qaswllsade
      241 yarqaatpgs wksrflsvfw easweprgfs acapftfdms vfgwtlpllr crgtilqfgp
      301 ylsvvrsktv fgrfvsvmsm tpegpfrqhi vhrifakpgl fawvacmgme vgsanmlerd
      361 fiimankivl knpslvkeen glpkfrrwye qfysansptw qdvreqslqw
//
LOCUS       XP_077509922             385 aa            linear   INV 25-NOV-2025
DEFINITION  cholesterol 7-desaturase nvd-like [Amblyomma americanum].
ACCESSION   XP_077509922
VERSION     XP_077509922.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653796.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..385
                     /product="cholesterol 7-desaturase nvd-like"
                     /calculated_mol_wt=43503
     Region          42..383
                     /region_name="PobA"
                     /note="Chlorophyllide a oxygenase/letal leaf spot protein
                     [Coenzyme transport and metabolism]; COG5749"
                     /db_xref="CDD:444459"
     CDS             1..385
                     /gene="LOC144120960"
                     /coded_by="XM_077653796.1:1..1158"
                     /db_xref="GeneID:144120960"
ORIGIN      
        1 mvpsrggppp kvkvkvngqt knaskksqek tksrrampga ippvfpngwi pvlesselvv
       61 gkiksisvlg qelvavrced gtarvfdayc phlgahlgvm grvvgdciec pfhgwrfdck
      121 dgtctnvpys akapsfvkak awpckellgl vfvwyhadge eptwdvpedi lgetleseat
      181 grfehlvhch iqdiaengad lghfnqihka ssfvggdefq ktlgeswkgr lfqhhwtatw
      241 spkkheaavi iessisilgw kpnylrhhie arqvgpalva icvwgrtghy vaiqslrpea
      301 pfdirvvhrl yfepqvswll rkfytmtlrn mvdrdvavwn yktylrrpal vkedqsiaay
      361 rkwysqfysa ssptwqeire qslew
//
LOCUS       XP_077509947             823 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 2-like [Amblyomma americanum].
ACCESSION   XP_077509947
VERSION     XP_077509947.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653821.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..823
                     /product="endothelin-converting enzyme 2-like"
                     /calculated_mol_wt=90603
     Region          114..747
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..823
                     /gene="LOC144120975"
                     /coded_by="XM_077653821.1:1..2472"
                     /db_xref="GeneID:144120975"
ORIGIN      
        1 mngqlavprr hewwaqrmws rmagegceap sttsggldrr qravalghll ssllgvaltl
       61 saitiglsvl rhqrgsegsg vfgmgrsswn tcnsklcmah frrlkdslnr stapcgdiyr
      121 facgawhpra aksetvfrdl lavaqqdtir yleavndasn kmvlptgkvl lpdsrpvlri
      181 nraerayqvc lhsgsstkda alltefladr slswpertta paqpldvlld ldinwnlglw
      241 fsvrvspipg rnqrqvrvgs givsydwkra ldsmvtrgvy karvrrfhda lrarsfaams
      301 dkemeelrry estivgalcg svsggqnela fplkqmqnla tprlataqwd tllnwqlnka
      361 lrvteadrvi atnvkllqav dyllsshpae vvlhqlawsl vqmlgwmads alpgrstaye
      421 mgdmrsadcf wathrafgvt llsgyintty plhwrssvda llmtitqtai nlflktswid
      481 aesraaavdk lrrmntslwp sdsylnasev aailenfpel redgtvlryw ldalsarral
      541 lgrqwdvada fypgdlqppr alfryhyyln eqavflhalr splfveglgl avnmgglgah
      601 yaaalarafd prgvlldgrg sisglwwgks syrdyerrta cpataasgae ggvqafegva
      661 aielayqafn asrsiadqgt rthqrrrifl glsedqaffv afcqascarg pserqqlyct
      721 lplknfrefa taftcpvasp mnpalrcgff easdqraark nggesedagk sastnasanv
      781 gadgavtswt rpparrsgsd dqdpltlapt glaghmvrqp pvl
//
LOCUS       XP_077509962             802 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 2-like [Amblyomma americanum].
ACCESSION   XP_077509962
VERSION     XP_077509962.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653836.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..802
                     /product="endothelin-converting enzyme 2-like"
                     /calculated_mol_wt=87809
     Region          93..726
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..802
                     /gene="LOC144120976"
                     /coded_by="XM_077653836.1:1..2409"
                     /db_xref="GeneID:144120976"
ORIGIN      
        1 magegceaps ttsggldrrq ravalghlls sllgvaltls aitiglsvlr hqrgsegsgv
       61 fgmgrsswnt cnsklcmahf rrlkdslnrs tapcgdiyrf acgawhpraa ksetvfrdll
      121 avaqqdtiry leavndasnk mvlptgkvll pdsrpvlrin raerayqvcl hsgsstkdaa
      181 lltefladrs lswperttap aqpldvlldl dinwnlglwf svrvspipgr nqrqvrvgsg
      241 ivsydwkral dsmvtrgvyk arvrrfhdal rarsfaamsd kemeelrrye stivgalcgs
      301 vsggqnelaf plkqmqnlat hrlataqwdt llnwqlnkal rvteadrvia tnvkllqavd
      361 yllsslpaev vlhqlarslv qmigwmadsa lpgrptayem edmrsadcfw vthrafgvth
      421 lsgyinttyp lhwrssvdai lmtitqtain fflktswiha esraaavdkl rrmntslwps
      481 dsylnaseva ailenfpepr edgavlrywl dalsarrall grqwdladaf ypgdlqppra
      541 lfcyhyylne lavslhalrs plfvegvgla vnmgglgahy avalarafdp rgvlldgrgs
      601 tsglwwgkss yrdyerrtac pataasgaeg gvqafegvaa ieiayqafna srsiadlgtr
      661 thqrrriflg lsedqafiva fcqascargp serqelsctl plknfrefat aftcpvaspm
      721 npalrcgffe asdqraarkn ggesedagkt astnasanvg adgavtswtr pparqsgsdd
      781 qdpltlattg laghmvrqpp vl
//
LOCUS       XP_077509974             375 aa            linear   INV 25-NOV-2025
DEFINITION  neprilysin-1-like [Amblyomma americanum].
ACCESSION   XP_077509974
VERSION     XP_077509974.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653848.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..375
                     /product="neprilysin-1-like"
                     /calculated_mol_wt=40507
     Region          <27..299
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..375
                     /gene="LOC144120984"
                     /coded_by="XM_077653848.1:1..1128"
                     /db_xref="GeneID:144120984"
ORIGIN      
        1 miligwrqva sfeagasigl rrhlglktsw ihaesraaav dklrrmntsl wpsdsylnas
       61 evaailenfp epredgavlr ywldalsarr allgrqwdva dalypgdlqp pralfryhyy
      121 lnelavslha lrsplfvegv glavnmgglg ahyavalara fdprgvlldg rgstsglwwg
      181 kssyrdyerr tacpataasg aeggvqafeg vaaieiayga fnasrsiadq gtrthqrrri
      241 flglsedqaf fvafcqasca rgpnerqqms ctlplknfre fataftcpva spmnpalrcg
      301 ffeasdqraa rknggeseda gkrastnasa nvgadgavts wtrpparrsg sddqdpltla
      361 ttglaghmvr qppvl
//
LOCUS       XP_077510113             458 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-4-like [Amblyomma americanum].
ACCESSION   XP_077510113
VERSION     XP_077510113.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077653987.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..458
                     /product="protein argonaute-4-like"
                     /calculated_mol_wt=52011
     Region          10..>361
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          <359..>412
                     /region_name="Piwi-like"
                     /note="PIWI domain. Domain found in proteins involved in
                     RNA silencing. RNA silencing refers to a group of related
                     gene-silencing mechanisms mediated by short RNA molecules,
                     including siRNAs, miRNAs, and heterochromatin-related
                     guide RNAs. The central component...; cl00628"
                     /db_xref="CDD:412485"
     CDS             1..458
                     /gene="LOC144121056"
                     /coded_by="XM_077653987.1:1..1377"
                     /db_xref="GeneID:144121056"
ORIGIN      
        1 mrvcilvcav lslspitsqe lgrtvpshfp rrpahgqlgr piqlianhfs ieiptgsvyh
       61 yvvgifseta ketkvpeqkv lihqhedqqd shrapmdlee dhriqkiiik iqyavtvnld
      121 alhgvfqnrv nnvpqeviqa idivlrffsd srcvrtpadr slhdnqyvrl nreleglrvk
      181 lkhlpyprky kvvkitreaa kdvcfesegs qtsvadyfqs ryrrlsypnl pcvqggspsh
      241 pvyipldvce lavgqhcrkk ldenqttemi krtakspakr fqeirqsvrd lvssmdeylr
      301 efgvkintep tqvmgrmldp psldfenntm ckprygtwel rgqrfykams mtqwivhnvs
      361 airlacsems ptetyepplt fivvqkrhht rfmpandsdg vgkfrnvppg tthiasklet
      421 cgvvsessgg cgdavlttae yveavkmlqe lqasmyfv
//
LOCUS       XP_077510126             313 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121064 [Amblyomma americanum].
ACCESSION   XP_077510126
VERSION     XP_077510126.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654000.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..313
                     /product="uncharacterized protein LOC144121064"
                     /calculated_mol_wt=35081
     Region          129..263
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     CDS             1..313
                     /gene="LOC144121064"
                     /coded_by="XM_077654000.1:1..942"
                     /db_xref="GeneID:144121064"
ORIGIN      
        1 mdvlkkkrka vrsqatrltk etddilaaee aptnekvsvl lerlrlvkqq lndvdaaiep
       61 nvkddeaeae feqvieygdk iatcicrlnl kkeqteqask dkeirfqatv gsasaaqrik
      121 lpkleltkfd gkrknwqpfw eqfevavhkn telsnidrln ylktlltgea vtaiaglqas
      181 gdcysdavdi lrqrfgspea lvqdhmqgli dvkpvfsarn vralrglyde iqvhmrglka
      241 lgigddgyqa mlypvllral pkelildyhr shaenqeeat pavvddsgst tsssdssqlt
      301 plhsllrffr fel
//
LOCUS       XP_077510139             510 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121070 [Amblyomma americanum].
ACCESSION   XP_077510139
VERSION     XP_077510139.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654013.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..510
                     /product="uncharacterized protein LOC144121070"
                     /calculated_mol_wt=56072
     Region          182..211
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          212..246
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          269..298
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          299..321
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     CDS             1..510
                     /gene="LOC144121070"
                     /coded_by="XM_077654013.1:47..1579"
                     /db_xref="GeneID:144121070"
ORIGIN      
        1 merveqfgds wflllaedyi vlkletpwpa dvplpggysm rdddgrllvt nqaslvsats
       61 slretrisea lfagcaafes snvyeasare vlrfagevci vhnrdtifrl irmfpnakvl
      121 slphdlcvha veldepsrir gaplvptsel relegstqig cassllftee tvlqilrtcp
      181 nvrrfdvasv qraflqlans sppggrhnas nfthlmlgfe akisncghvf tagaddiela
      241 agkfpllevl qvivgslpaf aaisafrylr slsvslagsi rfadvgaelq sllktwprle
      301 slslwccggl rlaslamlcp elktlrlvsc eissddtpld ygafpqlrtv qlcvklpmvv
      361 fdgffyatsg tlrnlliagd aicrgflhlc clhdrqisfp cleeltldta ltvvalqltp
      421 erlhhllnsl palrhlstds ydlrlfveny cvprgsvsls clalfggmda iahtgtcsgy
      481 ldpartqrye rpkskrrrqg skvqlcdqqn
//
LOCUS       XP_077510148            1984 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121077 [Amblyomma americanum].
ACCESSION   XP_077510148
VERSION     XP_077510148.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654022.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1984
                     /product="uncharacterized protein LOC144121077"
                     /calculated_mol_wt=221357
     Region          222..299
                     /region_name="ArgoN"
                     /note="N-terminal domain of argonaute; pfam16486"
                     /db_xref="CDD:465134"
     Region          309..360
                     /region_name="ArgoL1"
                     /note="Argonaute linker 1 domain; pfam08699"
                     /db_xref="CDD:462567"
     Region          363..477
                     /region_name="PAZ_argonaute_like"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; cd02846"
                     /db_xref="CDD:239212"
     Site            order(415,430,439,443,460,467,469)
                     /site_type="other"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]"
                     /db_xref="CDD:239212"
     Region          522..925
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(654,658,670..673,676,685,688,692,696)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Region          1121..1983
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          1511..1940
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(1643,1647,1659..1662,1665,1674,1677,1681,1685)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(1712,1714,1790,1930)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..1984
                     /gene="LOC144121077"
                     /coded_by="XM_077654022.1:1..5955"
                     /db_xref="GeneID:144121077"
ORIGIN      
        1 mssvpsasgg nqatppapsp agpswvsfaq alqqgaaasp qpravsggpt taadpsahpt
       61 aqpsasrpsy gaaaaagsak vasisprpas aaaavsgtqp aaaalppspp stppvavtqq
      121 tpaagdqats gddvrikele rtlpshfprr pahgqlgrti qllanhfsie iptgsvyhyd
      181 vdissetake tkvpeqkkyr clstkinria iellvkkyrq dlancipafd grknlytrrq
      241 lnfrertftv dleedqrsek fiikiqyaat vnldalhgvf qkrvqtvpqe vlqaidivlr
      301 hspsinlapv grsffrppgp nehndlgggr evwfgyytsv rpaqwkpmln vdmsattfye
      361 slplvdfmcr flsdsrrvlt padfrslrdn qyvrlnrelk glrvkvthlp yprkykvvki
      421 trepakeiyf esegsqisva dyfqsryrrl sypnfpcvqs gspthpvyip levcelaegq
      481 hcrkkldesq taemikhtak ppakrfqkir qsvrdmvsss dkylrefgvk inteptqvvg
      541 rvldppslvf enntmckprd gtwdlrgqrf ykamsmtkwi vlnvsrsahk dslenfirml
      601 irigqelgmr iaqplavitf dtnrqpmrtv lteqrkqypq lemvvavitk atnyaeikqv
      661 aeteislrtq cildnnvvqk cnaaliqnlc qkinakmggi nnsllmqekp klfhtpvivi
      721 gadvshpspg dkvrpsiaac vgsldtvpsk fhatirvqie dseakarvei irdlkdmike
      781 lllafhratr hkperivfyr dgvsegqfle vrnyevsair lackelspne tyepaitfiv
      841 vqkrhhtrfm pandrdgvgk crnvppgttv dsvvthpldf dfflcshfgi qgtsrpahyy
      901 vvwddskfsa ddlqklsfyl chtyvqgahc pldkepfqee dmawttisrg nvlgrkvrrw
      961 nsehgcdaec vasamlehfa ntyqyhamis psvpsasggn qatppapspa gpswvsfaqa
     1021 lqqgaaaspq pravsggptt aadpsahpta qpsasrpsyg aaaaagsakv asisprpasa
     1081 aaavsgtqpa aaalppspps tppvavtqqt paagdqatsg ddvrikeler tlpshfprrp
     1141 ahgqlgrtiq llanhfsiei ptgsvyhydv dissetaket kvpeqkkyrc lstkinriai
     1201 ellvkkyrqd lancipafdg rknlytrrql nfrertftvd leedqrsekf iikiqyaatv
     1261 nldalhgvfq krvqtvpqev lqaidivlrh spsinlapvg rsffrppgpn ehndlgggre
     1321 vwfgyytsvr paqwkpmlnv dmsattfyes lplvdfmcrf lsdsrrvltp adfrslrdnq
     1381 yvrlnrelkg lrvkvthlpy prkykvvkit repakeiyfe segsqisvad yfqsryrrls
     1441 ypnfpcvqsg spthpvyipl evcelaegqh crkkldesqt aemikhtakp pakrfqkirq
     1501 svrdmvsssd kylrefgvki nteptqvvgr vldppslvfe nntmckprdg twdlrgqrfy
     1561 kamsmtkwiv lnvsrsahkd slenfirmli rigqelgmri aqplavitfd tnrqpmrtvl
     1621 teqrkqypql emvvavitka tnyaeikqva etelslrtqc ildnnvvqkc naaliqnlcq
     1681 kinakmggin nsllmqekpk lfhtpvivig advshpspgd kvrpsiaacv gsldtvpskf
     1741 hatirvqied seakarveii rdlkdmikel llafhratrh kperivfyrd gvsegqflev
     1801 rnyevsairl ackelspnet yepaitfivv qkrhhtrfmp andrdgvgkc rnvppgttvd
     1861 svvthpldfd fflcshfgiq gtsrpahyyv vwddskfsad dlqklsfylc htysrcarsv
     1921 sipapvyyah laafrakhhi askletsgav sqssegggdt vlttaqyvea vkvlqelqas
     1981 myfv
//
LOCUS       XP_077510159            1084 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077510159
VERSION     XP_077510159.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654033.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1084
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=120069
     Region          221..1083
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          611..1040
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(743,747,759..762,765,774,777,781,785)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(812,814,890,1030)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..1084
                     /gene="LOC144121086"
                     /coded_by="XM_077654033.1:1..3255"
                     /db_xref="GeneID:144121086"
ORIGIN      
        1 malaehrrev aseirdalag ngrsmkevvm pecertalfm kdkvvealce drtlgdagfl
       61 asaasagkqa asvdgeakae eqlviaslni gavpsasggn qatppapspa gpswvsfaqa
      121 lqqgaaaspq pravsggptt aadpsahpta qpsasrpsyg aaaaagsakv asisprpasa
      181 aaavsgtqpa aaalppspps tppvavtqqt paagdqatsg ddvrikeler tlpshfprrp
      241 ahgqlgrtiq llanhfsiei ptgsvyhydv dissetaket kvpeqkkyrc lstkinriai
      301 ellvkkyrqd lancipafdg rknlytrrql nfrertftvd leedqrsekf iikiqyaatv
      361 nldalhgvfq krvqtvpqev lqaidivlrh spsinlapvg rsffrppgpn ehndlgggre
      421 vwfgyytsvr paqwkpmlnv dmsattfyes lplvdfmcrf lsdsrrvltp adfrslrdnq
      481 yvrlnrelkg lrvkvthlpy prkykvvkit repakeiyfe segsqisvad yfqsryrrls
      541 ypnfpcvqsg spthpvyipl evcelaegqh crkkldesqt aemikhtakp pakrfqkirq
      601 svrdmvsssd kylrefgvki nteptqvvgr vldppslvfe nntmckprdg twdlrgqrfy
      661 kamsmtkwiv lnvsrsahkd slenfirmli rigqelgmri aqplavitfd tnrqpmrtvl
      721 teqrkqypql emvvavitka tnyaeikqva etelslrtqc ildnnvvqkc naaliqnlcq
      781 kinakmggin nsllmqekpe lfhtpvivig advshpspgd kvrpsiaacv gsldtvpskf
      841 hatirvqied seakarveii rdlkdmikel llafhratrh kperivfyrd gvsegqflev
      901 rnyevsairl ackelspnet yepaitfivv qkrhhtrfmp andrdgvgkc rnvppgttvd
      961 svvthpldfd fflcshfgiq gtsrpahyyv vwddskfsad dlqklsfylc htysrcarsv
     1021 sipapvyyah laafrakhhi askletsgav sqssegggdt vlttaqyvea vkvlqelqas
     1081 myfv
//
LOCUS       XP_077510170             995 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077510170
VERSION     XP_077510170.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654044.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..995
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=110714
     Region          132..994
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          522..951
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(654,658,670..673,676,685,688,692,696)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(723,725,801,941)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..995
                     /gene="LOC144121094"
                     /coded_by="XM_077654044.1:1..2988"
                     /db_xref="GeneID:144121094"
ORIGIN      
        1 mssvpsasgg nqatppapsp agpswvsfaq alqqgaaasp qpravsggpt taadpsahpt
       61 aqpsasrpsy gaaaaagsak vasisprpas aaaavsgtqp aaaalppspp stppvavtqq
      121 tpaagdqats gddvrikele rtlpshfprr pahgqlgrti qllanhfsie iptgsvyhyd
      181 vdissetake tkvpeqkkyr clstkinria iellvkkyrq dlancipafd grknlytrrq
      241 lnfrertftv dleedqrsek fiikiqyaat vnldalhgvf qkrvqtvpqe vlqaidivlr
      301 hspsinlapv grsffrppgp nehndlgggr evwfgyytsv rpaqwkpmln vdmsattfye
      361 slplvdfmcr flsdsrrvlt padfrslrdn qyvrlnrelk glrvkvthlp yprkykvvki
      421 trepakeiyf esegsqisva dyfqsryrrl sypnfpcvqs gspthpvyip levcelaegq
      481 hcrkkldesq taemikhtak ppakrfqkir qsvrdmvsss dkylrefgvk inteptqvvg
      541 rvldppslvf enntmckprd gtwdlrgqrf ykamsmtkwi vlnvsrsahk dslenfirml
      601 irigqelgmr iaqplavitf dtnrqpmrtv lteqrkqypq lemvvavitk atnyaeikqv
      661 aetelslrtq cildnnvvqk cnaaliqnlc qkinakmggi nnsllmqekp elfhtpvivi
      721 gadvshpspg dkvrpsiaac vgsldtvpsk fhatirvqie dseakarvei irdlkdmike
      781 lllafhratr hkperivfyr dgvsegqfle vrnyevsair lackelspne tyepaitfiv
      841 vqkrhhtrfm pandrdgvgk crnvppgttv dsvvthpldf dfflcshfgi qgtsrpahyy
      901 vvwddskfsa ddlqklsfyl chtysrcars vsipapvyya hlaafrakhh iaskletsga
      961 vsqssegggd tvlttaqyve avkvlqelqa smyfv
//
LOCUS       XP_077510181            1055 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077510181
VERSION     XP_077510181.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654055.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1055
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=116889
     Region          192..1054
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          582..1011
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(714,718,730..733,736,745,748,752,756)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(783,785,861,1001)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..1055
                     /gene="LOC144121103"
                     /coded_by="XM_077654055.1:1..3168"
                     /db_xref="GeneID:144121103"
ORIGIN      
        1 mpecertalf mkdkvvealc edrtlgdagf lasaasagkq aasvdgeaka eeqlviasln
       61 igavpsasgg nqatppapsp agpswvsfaq alqqgaaasp qpravsggpt taadpsahpt
      121 aqpsasrpsy gaaaaagsak vasisprpas aaaavsgtqp aaaalppspp stppvavtqq
      181 tpaagdqats gddvrikele rtlpshfprr pahgqlgrti qllanhfsie iptgsvyhyd
      241 vdissetake tkvpeqkkyr clstkinria iellvkkyrq dlancipafd grknlytrrq
      301 lnfrertftv dleedqrsek fiikiqyaat vnldalhgvf qkrvqtvpqe vlqaidivlr
      361 hspsinlapv grsffrppgp nehndlgggr evwfgyytsv rpaqwkpmln vdmsattfye
      421 slplvdfmcr flsdsrrvlt padfrslrdn qyvrlnrelk glrvkvthlp yprkykvvki
      481 trepakeiyf esegsqisva dyfqsryrrl sypnfpcvqs gspthpvyip levcelaegq
      541 hcrkkldesq taemikhtak ppakrfqkir qsvrdmvsss dkylrefgvk inteptqvvg
      601 rvldppslvf enntmckprd gtwdlrgqrf ykamsmtkwi vlnvsrsahk dslenfirml
      661 irigqelgmr iaqplavitf dtnrqpmrtv lteqrkqypq lemvvavitk atnyaeikqv
      721 aetelslrtq cildnnvvqk cnaaliqnlc qkinakmggi nnsllmqekp klfhtpvivi
      781 gadvshpspg dkvrpsiaac vgsldtvpsk fhatirvqie dseakarvei irdlkdmike
      841 lllafhratr hkperivfyr dgvsegqfle vrnyevsair lackelspne tyepaitfiv
      901 vqkrhhtrfm pandrdgvgk crnvppgttv dsvvthpldf dfflcshfgi qgtsrpahyy
      961 vvwddskfsa ddlqklsfyl chtysrcars vsipapvyya hlaafrakhh iaskletsga
     1021 vsqssegggd tvlttaqyve avkvlqelqa smyfv
//
LOCUS       XP_077510193             994 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077510193
VERSION     XP_077510193.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654067.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..994
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=111412
     Region          131..993
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          521..950
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(653,657,669..672,675,684,687,691,695)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(722,724,800,940)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..994
                     /gene="LOC144121112"
                     /coded_by="XM_077654067.1:1..2985"
                     /db_xref="GeneID:144121112"
ORIGIN      
        1 mpecertalf mkdkvvealc edrtlgdagf lasaasagkq aasvdgeaka eeqlviasln
       61 iggdfldnyg aaaaagsakv afisprpasa aaavsgtqpa aaalppspps tppvavtqqt
      121 paagdqatsg ddvrikeler tlpshfprrp ahgqlgrtiq llanhfsiei ptgsvyhydv
      181 dissetaket kvpeqkkyrc lstkinriai ellvkkyrqd lancipafdg rknlytrrql
      241 nfrertftvd leedqrsekf iikiqyaatv nldalhgvfq krvqtvpqev lqaidivlrh
      301 rpsinlapvg rsffrppgpn ehndlgggre vwfgyytsvr paqwkpmlnv dmsattfyes
      361 lplvdfmcrf lsdsrrvltp adfrslrdnq yvrlnrelkg lrvkvthlpy prkykvvkit
      421 repakeiyfe segsqisvad yfqsryrrls ypnfpcvqsg spthpvyipl evcelaegqh
      481 crkkldesqt aemikhtakp pakrfqkirq svrdmvsssd kylrefgvki nteptqvvgr
      541 vldppslvfe nntmckprdg twdlrgqrfy kamsmtkwiv lnvsrsahkd slenfirmli
      601 rigqelgmri aqplavitfd tnrqpmrtvl teqrkqypql emvvavitka tnyaeikqva
      661 etelslrtqc ildnnvvqkc naaliqnlcq kinakmggin nsllmqekpk lfhtpvivig
      721 advshpspgd kvrpsiaacv gsldtvpskf hatirvqied seakarveii rdlkdmikel
      781 llafhratrh kperivfyrd gvsegqflev rnyevsairl ackelspnet yepaitfivv
      841 qkrhhtrfmp andrdgvgkc rnvppgttvd svvthpldfd fflcshfgiq gtsrpahyyv
      901 vwddsrfsad dlqklsfylc htysrcarsv sipapvyyah laafrakhhi askletsgav
      961 sqssegggdt vlttaqyvea vkvlqelqas myfv
//
LOCUS       XP_077510206            1084 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077510206
VERSION     XP_077510206.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654080.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1084
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=120095
     Region          221..1083
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          611..1040
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(743,747,759..762,765,774,777,781,785)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(812,814,890,1030)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..1084
                     /gene="LOC144121118"
                     /coded_by="XM_077654080.1:1..3255"
                     /db_xref="GeneID:144121118"
ORIGIN      
        1 malaehrrev aseirdalag ngrsmkevvm pecertalfm kdkvvealce drtlgdagfl
       61 asaasagkqa asvdgeakae eqlviaslni gavpsasggn qatppapspa gpswvsfaqa
      121 lqqgaaaspq pravsggptt aadpsahpta qpsasrpsyg aaaaagsakv asisprpasa
      181 aaavsgtqpa aaalppspps tppvavtqqt paagdqatsg ddvrikeler tlpshfprrp
      241 ahgqlgrtiq llanhfsiei ptgsvyhydv dissetaket kvpeqkkyrc lstkinriai
      301 ellvkkyrqd lancipafdg rknlytrrql nfrertftvd leedqrsekf iikiqyaatv
      361 nldalhgvfq krvqtvpqev lqaidivlrh spsinlapvg rsffrppgpn ehndlgggre
      421 vwfgyytsvr paqwkpmlnv dmsattfyes lplvdfmcrf lsdsrrvltp adfrslrdnq
      481 yvrlnrelkg lrvkvthlpy prkykvvkit repakeiyfe segsqisvad yfqsryrrls
      541 ypnfpcvqsg spthpvyipl evcelaegqh crkkldesqt aemikhtakp pakrfqkirq
      601 svrdmvsssd kylrefgvki nteptqvvgr vldppslvfe nntmckprdg twdlrgqrfy
      661 kamsmtkwiv lnvsrsahkd slenfirmli rigqelgmri aqplavitfd tnrqpmrtvl
      721 teqrkqypql emvvavitka tnyaeikqva etelslrtqc ildnnvvqkc naaliqnlcq
      781 kinakmggin nsllmqekpe lfhtpvivig advshpspgd kvrpsiaacv gsldtvpskf
      841 hatirvqied seakarveii rdlkdmikel llafhratrh kperivfyrd gvsegqflev
      901 rnyevsairl ackelspnet yepaitfivv qkrhhtrfmp andrdgvgkc rnvppgttvd
      961 svvthpldfd fflcshfgiq gtsrpahyyv vwddskfsad dlqklsfylc htysrcarsv
     1021 sipapvyyah laafrakhhi askletsgav sqlsegggdt vlttaqyvea vkvlqelqas
     1081 myfv
//
LOCUS       XP_077510221             477 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121124 [Amblyomma americanum].
ACCESSION   XP_077510221
VERSION     XP_077510221.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654095.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 14% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..477
                     /product="uncharacterized protein LOC144121124"
                     /calculated_mol_wt=51216
     Region          180..>298
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          <298..433
                     /region_name="Piwi-like"
                     /note="PIWI domain. Domain found in proteins involved in
                     RNA silencing. RNA silencing refers to a group of related
                     gene-silencing mechanisms mediated by short RNA molecules,
                     including siRNAs, miRNAs, and heterochromatin-related
                     guide RNAs. The central component...; cl00628"
                     /db_xref="CDD:412485"
     CDS             1..477
                     /gene="LOC144121124"
                     /coded_by="XM_077654095.1:1..1434"
                     /db_xref="GeneID:144121124"
ORIGIN      
        1 mawttisrgn vlgrkvrrwn sehgcdaecv asamlehfan tyqyhamisp svpsasggnq
       61 atppapspag pswvsfaqal qqgaaaspqp ravsggptta adpsahptaq psasrpsyga
      121 aaaagsakva sisprpasaa aavsgtqpaa aalppsppst ppvavtqqtp aagdqatsgd
      181 dvrikelert lpshfprrpa hgqlgrtiql lanhfsieip tgsvyhydvd issetaketk
      241 vpeqkkyrcl stkinriaie llvkkyrqdl ancipafdgr knlytrrqln fsertftvsa
      301 irlackelsp netyepaitf ivvqkrhhtr fmpandrdgv gkcrnvppgt tvdsvvthpl
      361 dfdfflcshf giqgtsrpah yyvvwddskf saddlqklsf ylchtysrca rsvsipapvy
      421 yahlaafrak hhiasklets gavsqssegg gdtvlttaqy veavkvlqel qasmyfv
//
LOCUS       XP_077510231             807 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-4-like [Amblyomma americanum].
ACCESSION   XP_077510231
VERSION     XP_077510231.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654105.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..807
                     /product="protein argonaute-4-like"
                     /calculated_mol_wt=91465
     Region          43..806
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          433..763
                     /region_name="Piwi-like"
                     /note="PIWI domain. Domain found in proteins involved in
                     RNA silencing. RNA silencing refers to a group of related
                     gene-silencing mechanisms mediated by short RNA molecules,
                     including siRNAs, miRNAs, and heterochromatin-related
                     guide RNAs. The central component...; cl00628"
                     /db_xref="CDD:412485"
     Site            order(565,569,581..584,587,596,599,603,607)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     CDS             1..807
                     /gene="LOC144121130"
                     /coded_by="XM_077654105.1:1..2424"
                     /db_xref="GeneID:144121130"
ORIGIN      
        1 mssaavsgtq paaaalppsp prtppvavtq qtpaagdqat sgddvrikel ertlpshfpr
       61 rpahgqlgrt iqllanhfsi eiptgsvyhy dvdissetak etkvpeqkky rclstkinri
      121 aiellvkkyr qdlancipaf dgrknlytrr qlnfrertft vdleedqrse kfiikiqyaa
      181 tvnldalhgv fqkrvqtvpq evlqaidivl rhrpsinlap vgrsffrppg pnehndlggg
      241 revwfgyyts vrpaqwkpml nvdmsattfy eslplvdfmc rflsdsrrvl tpadfrslrd
      301 nqyvrlnrel kglrvkvthl pyprkykvvk itrepakeiy fesegsqisv adyfqsryrr
      361 lsypnfpcvq sgspthpvyi plevcelaeg qhcrkkldes qtaemikhta kppakrfqki
      421 rqsvrdmvss sdkylrefgv kinteptqvv grvldppslv fenntmckpr dgtwdlrgqr
      481 fykamsmtkw ivlnvsrsah kdslenfirm lirigqelgm riaqplavit fdtnrqpmrt
      541 vlteqrkqyp qlemvvavit katnyaeikq vaetelslrt qcildnnvvq kcnaaliqnl
      601 cqkmnakmgg inngllmqek pklfhtpvsa irlackelsp netyepaitf ivvqkrhhtr
      661 fmpandrdgv gkcrnvppgt tvdsvvthpl dfdfflcshf giqgtsrpah yyvvwddskf
      721 saddlqklsf ylchtysrca rsvsipapvy yahlaafrak hhiasklets gavsqssegg
      781 gdtvlttaqy veavkvlqel qasmyfv
//
LOCUS       XP_077510242            1055 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077510242
VERSION     XP_077510242.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654116.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1055
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=116889
     Region          192..1054
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          582..1011
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(714,718,730..733,736,745,748,752,756)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(783,785,861,1001)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..1055
                     /gene="LOC144121137"
                     /coded_by="XM_077654116.1:1..3168"
                     /db_xref="GeneID:144121137"
ORIGIN      
        1 mpecertalf mkdkvvealc edrtlgdagf lasaasagkq aasvdgeaka eeqlviasln
       61 igavpsasgg nqatppapsp agpswvsfaq alqqgaaasp qpravsggpt taadpsahpt
      121 aqpsasrpsy gaaaaagsak vasisprpas aaaavsgtqp aaaalppspp stppvavtqq
      181 tpaagdqats gddvrikele rtlpshfprr pahgqlgrti qllanhfsie iptgsvyhyd
      241 vdissetake tkvpeqkkyr clstkinria iellvkkyrq dlancipafd grknlytrrq
      301 lnfrertftv dleedqrsek fiikiqyaat vnldalhgvf qkrvqtvpqe vlqaidivlr
      361 hspsinlapv grsffrppgp nehndlgggr evwfgyytsv rpaqwkpmln vdmsattfye
      421 slplvdfmcr flsdsrrvlt padfrslrdn qyvrlnrelk glrvkvthlp yprkykvvki
      481 trepakeiyf esegsqisva dyfqsryrrl sypnfpcvqs gspthpvyip levcelaegq
      541 hcrkkldesq taemikhtak ppakrfqkir qsvrdmvsss dkylrefgvk inteptqvvg
      601 rvldppslvf enntmckprd gtwdlrgqrf ykamsmtkwi vlnvsrsahk dslenfirml
      661 irigqelgmr iaqplavitf dtnrqpmrtv lteqrkqypq lemvvavitk atnyaeikqv
      721 aetelslrtq cildnnvvqk cnaaliqnlc qkinakmggi nnsllmqekp klfhtpvivi
      781 gadvshpspg dkvrpsiaac vgsldtvpsk fhatirvqie dseakarvei irdlkdmike
      841 lllafhratr hkperivfyr dgvsegqfle vrnyevsair lackelspne tyepaitfiv
      901 vqkrhhtrfm pandrdgvgk crnvppgttv dsvvthpldf dfflcshfgi qgtsrpahyy
      961 vvwddskfsa ddlqklsfyl chtysrcars vsipapvyya hlaafrakhh iaskletsga
     1021 vsqssegggd tvlttaqyve avkvlqelqa smyfv
//
LOCUS       XP_077510252            1055 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077510252
VERSION     XP_077510252.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654126.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1055
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=116919
     Region          192..1054
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          582..1011
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(714,718,730..733,736,745,748,752,756)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(783,785,861,1001)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..1055
                     /gene="LOC144121141"
                     /coded_by="XM_077654126.1:1..3168"
                     /db_xref="GeneID:144121141"
ORIGIN      
        1 mpecertalf mkdkvvealc edrtlgdagf lasaasagkq aasvdgeaka eeqlviasln
       61 igavpsasgg nqatppapsp agpswvsfaq alqqgaaasp qpravsgglt taadpsahpt
      121 aqpsasrpsy gaaaaagsak vasisprpas aaaavsgtqp aaaalppspp stppvavtqq
      181 tpaagdqats gddvrikele rtlpshfprr pahgqlgrti qllanhfsie iptgsvyhyd
      241 vdissetake tkvpeqknyr clstkinriv iellvkkyrq dlancipafd grknlytrrq
      301 lnfrertftv dleedqrsek fiikiqyaat vnldalhgvf qkrvqtvpqe vlqaidivlr
      361 hspsinlapv grsffrppgp nehndlgggr evwfgyytsv rpaqwkpmln vdmsattfye
      421 slplvdfmcr flsdsrrvlt padfrslrdn qyvrlnrelk glrvkvthlp yprkykvvki
      481 trepakeiyf esegsqisva dyfqsryrrl sypnfpcvqs gspthpvyip levcelaegq
      541 hcrkkldesq taemikhtak ppakrfqkir qsvrdmvsss dkylrefgvk inteptqvvg
      601 rvldppslvf enntmckprd gtwdlrgqrf ykamsmtkwi vlnvsrsahk dslenfirml
      661 irigqelgmr iaqplavitf dtnrqpmrtv lteqrkqypq lemvvavitk atnyaeikqv
      721 aetelslrtq cildnnvvqk cnaaliqnlc qkinakmggi nnsllmqekp klfhtpvivi
      781 gadvshpspg dkvrpsiaac vgsldtvpsk fhatirvqie dseakarvei irdlkdmike
      841 lllafhratr hkperivfyr dgvsegqfle vrnyevsair lackelspne tyepaitfiv
      901 vqkrhhtrfm pandrdgvgk crnvppgttv dsvvthpldf dfflcshfgi qgtsrpahyy
      961 vvwddskfsa ddlqklsfyl chtysrcars vsipapvyya hlaafrakhh iaskletsga
     1021 vsqssegggd tvlttaqyve avkvlqelqa smyfv
//
LOCUS       XP_077510266             995 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077510266
VERSION     XP_077510266.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654140.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..995
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=110788
     Region          132..994
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          522..951
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(654,658,670..673,676,685,688,692,696)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(723,725,801,941)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..995
                     /gene="LOC144121144"
                     /coded_by="XM_077654140.1:1..2988"
                     /db_xref="GeneID:144121144"
ORIGIN      
        1 mssvpsasgg nqatppapsp agpswvsfaq alqqgaaasp qpravsggpt taadpsahpt
       61 aqpsasrpsy gaaaaagsak vasssprpas aaaavsgtqp aaaalppspp stppvaltqq
      121 tpaagdqats gddvrikele rtlpshfprr pahgqlgrti qllanhfsie iptgsvyhyd
      181 vdissetake tkvpeqkkyr clstkinriv iellvkkyrq dlancipafd grknlytrhq
      241 lnfrertftv dleedqrsek fiikiqyaat vnldalhgvf qkrvqtvpqe vlqaidivlr
      301 hspsinlapv grsffrppgp nehndlgggr evwfgyytsv rpaqwkpmln vdmsattfye
      361 slplvdfmcr flsdsrrvlt padfrslrdn qyvrlnrelk glrvkvthlp yprkykvvki
      421 trepakeiyf esegsqisva dyfqsryrrl sypnfpcvqs gspthpvyip levcelaegq
      481 hcrkkldesq taemikhtak ppakrfqkir qsvrdmvsss dkylrefgvk inteptqvvg
      541 rvldppslvf enntmckprd gtwdlrgqrf ykamsmtkwi vlnvsrfahk dslenfirml
      601 irigqelgmr iaqplavitf dtnrqpmrtv lteqrkqypq lemvvavitk atnyaeikqv
      661 aetelslrtq cildnnvvqk cnaaliqnlc qkinakmggi nnsllmqekp klfhtpvivi
      721 gadvshpspg dkvrpsiaac vgsldtvpsk fhatirvqie dseakarvei irdlkdmike
      781 lllafhratr hkperivfyr dgvsegqfle vrnyevsair lackelspne tyepaitfiv
      841 vqkrhhtrfm pandrdgvgk crnvppgttv dsvvthpldf dfflcshfgi qgtsrpahyy
      901 vvwddskfsa ddlqklsfyl chtysrcars vsipapvyya hlaafrakhh iaskletsga
      961 vsqssegggd tvlttaqyve avkvlqemqa smyfv
//
LOCUS       XP_077510300             658 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121164 [Amblyomma americanum].
ACCESSION   XP_077510300
VERSION     XP_077510300.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654174.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..658
                     /product="uncharacterized protein LOC144121164"
                     /calculated_mol_wt=72049
     Region          <22..233
                     /region_name="RT_nLTR_like"
                     /note="RT_nLTR: Non-LTR (long terminal repeat)
                     retrotransposon and non-LTR retrovirus reverse
                     transcriptase (RT). This subfamily contains both non-LTR
                     retrotransposons and non-LTR retrovirus RTs. RTs catalyze
                     the conversion of single-stranded RNA into...; cd01650"
                     /db_xref="CDD:238827"
     Site            order(58..63,117..118,150,152..153,229..230)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238827"
     Site            order(58..63,117,152)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238827"
     Site            118
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238827"
     Region          436..563
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     Site            order(439..442,472..474,477,502,541,559)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260008"
     CDS             1..658
                     /gene="LOC144121164"
                     /coded_by="XM_077654174.1:1..1977"
                     /db_xref="GeneID:144121164"
ORIGIN      
        1 mlsyslsqfl gghrhwplpg qqsgfrhhrc tadsiadlvs tledaklnch vlclalldiq
       61 gafdnirhea ilealnimsi snnlleyvrg fladhrgcvr lgnvtsslrh iwrgvpqgsv
      121 lspllfnavl arlpnflpkd lrlpihvaih addiaiwrrg lrrcrvhtik nvqrainsva
      181 afleraglsi spsktqakll ntwvgarpgh tflhvggrdl twsrqvkylg llidvrltwn
      241 pavrhflaqs sriqgairsl lakgngitpr lglqlyqgma vpqstyalpl vrltvaqhlt
      301 ierklraair lclglprssp vaatlaeags wplellsrrt slrhlerlhn eidgrplldr
      361 lltrpnskmg qvaqyfdlvg gqpesfplpp rpdlghlyev hlslpgsrtr rntaavglrq
      421 evaavlqaep hgtvpiftdg svlpgpcpsa aaactapslh ssrqarlpfv assttaelaa
      481 ihlaadilne hpgikeaaiy tdsraalqll lrpfkgppia rrlawrldel ctrgtrirlt
      541 lvpahvgvlg neeadslakv ahspgtplsk nlcsldlars ivdrlllldh pdprvanghp
      601 thplpnlsks wpalqgaqpp pgaqgrlrqh rlsplpplrl pwvlplqlpr rdnpappv
//
LOCUS       XP_077510326             102 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121172 [Amblyomma americanum].
ACCESSION   XP_077510326
VERSION     XP_077510326.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654200.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..102
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..102
                     /product="uncharacterized protein LOC144121172"
                     /calculated_mol_wt=11115
     CDS             1..102
                     /gene="LOC144121172"
                     /coded_by="XM_077654200.1:226..534"
                     /db_xref="GeneID:144121172"
ORIGIN      
        1 mlktrhafae tfnrnlasia glrhstvfli craftdvyts atsavvrclr empilsaeiv
       61 rfnveltraa yaegvygshd dnrgesirth prpsakttsg gt
//
LOCUS       XP_077510382             298 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121183 [Amblyomma americanum].
ACCESSION   XP_077510382
VERSION     XP_077510382.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654256.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..298
                     /product="uncharacterized protein LOC144121183"
                     /calculated_mol_wt=32967
     CDS             1..298
                     /gene="LOC144121183"
                     /coded_by="XM_077654256.1:1..897"
                     /db_xref="GeneID:144121183"
ORIGIN      
        1 magghdlsss dvifggalss lvawykhtrl svpekqairn lrcnrsivvl padkgnatvl
       61 ldttdydekl cglledrtty talasdptgv lqrklqella dtfkfmppdr krlyyqllct
      121 ngsapaiywl lkihkpgvpl rpivdfsrsp lyslsnylhs vigplagraa thvanscdfv
      181 ekvrniqvde edimvsfdvt slftsmpvsm avskceellq rdttlaertp fdvpdlsnll
      241 riclsntyft ycsklyqqth dtamgasisv ttanivmeal etevlskftp qpklftat
//
LOCUS       XP_077510393             419 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121192 [Amblyomma americanum].
ACCESSION   XP_077510393
VERSION     XP_077510393.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654267.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 25% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..419
                     /product="uncharacterized protein LOC144121192"
                     /calculated_mol_wt=47385
     CDS             1..419
                     /gene="LOC144121192"
                     /coded_by="XM_077654267.1:1..1260"
                     /db_xref="GeneID:144121192"
ORIGIN      
        1 mayvavtlrg qpfesrtirk rssreslqea alfshknymg secaaparpa vhraaradrs
       61 rlvynsrfdt yvdvpeghtf sdgdkinlvg ssssdeqnfp letgtddgpl vlapscsepd
      121 krlftvqqpl yelppipldi kadiegttlg evssrtknri vswitahllt idwypgkfye
      181 paakalvlky pvlrdtigtg ydswrvafky kmgnirksvd ndpnveeark kfgrkgnfqd
      241 ksglkkqcrv rkwikinsps teelldkypa ltlremihqe fmeickvdfe rkllgfingh
      301 gqrlfsilkt srtaqkifse vqkaiadseg dakkycfavg vlqllphlvk eeafylrgpd
      361 sypcivmndk phtatnvcat fenikveain visgfatvme iywdmnieys kktrtpllf
//
LOCUS       XP_077510406             306 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121200 [Amblyomma americanum].
ACCESSION   XP_077510406
VERSION     XP_077510406.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654280.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..306
                     /product="uncharacterized protein LOC144121200"
                     /calculated_mol_wt=31902
     CDS             1..306
                     /gene="LOC144121200"
                     /coded_by="XM_077654280.1:42..962"
                     /db_xref="GeneID:144121200"
ORIGIN      
        1 mssavessai sgnegpdgpd vrlpsppprl psppprlpsp pprlpspppq lpspprlisp
       61 psllsshsme aatpaganct agcpagsast agdledveea tsdptmlkvl rllleirqqq
      121 reilqrvsrl eqarqeprtr spsppvsslp plcpqlpafs iedfeaaeaa vrddrvaaal
      181 kkqllrlggn nlkevaanvm gavmgvavqr lfslrgrkgk rpfvgtklcr vatdaicerq
      241 gvdilaaqgf igrwlpgacd rgggrkrrya qaleppesha qappanpcag sapcpgapsa
      301 scpatl
//
LOCUS       XP_077510419             372 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121207 [Amblyomma americanum].
ACCESSION   XP_077510419
VERSION     XP_077510419.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654293.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..372
                     /product="uncharacterized protein LOC144121207"
                     /calculated_mol_wt=38439
     CDS             1..372
                     /gene="LOC144121207"
                     /coded_by="XM_077654293.1:738..1856"
                     /db_xref="GeneID:144121207"
ORIGIN      
        1 mssavessai sgnegpdgpd vrlpsppprl psppprlpsp pprlpspppq lpspprlisp
       61 psllsshsme aatpaganct agcpagsast agdledveea tsdptmlkvl rllleirqqq
      121 reilqrvsrl eqarqeprtr spsppvsslp plcpqlpafs iedfeaaeaa vrddrvaaal
      181 kkqllrlggn nlkevaanvm gavmgvavqr lfslrgrkgk rpfvgtklcr vatdaicerq
      241 gvdilaaqgf igrwlpgacd rgggrkrrya qaleppesha qappanpcag sapcpgapsa
      301 scpatppgma ilspsgvhps sapptasssr patlhpssap spappptass srpatprstl
      361 atwviaprah ek
//
LOCUS       XP_077510428             179 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121215 [Amblyomma americanum].
ACCESSION   XP_077510428
VERSION     XP_077510428.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654302.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 44% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..179
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..179
                     /product="uncharacterized protein LOC144121215"
                     /calculated_mol_wt=20074
     CDS             1..179
                     /gene="LOC144121215"
                     /coded_by="XM_077654302.1:1..540"
                     /db_xref="GeneID:144121215"
ORIGIN      
        1 mgvqlgitgi sctdkvaawn rgpkrnvepa llnsmsfnlk rstvdpastq hprptrkvrh
       61 faskeeladh ikkspygelf nipgtllhnt lmttrreiqq psqapasvqr qqathhsekv
      121 afqspkckem qlnkveppvd dfeitlerpl cnltsescle tvdsflkdnk yehfkprga
//
LOCUS       XP_077510451             265 aa            linear   INV 25-NOV-2025
DEFINITION  microtubule-associated protein RP/EB family member 1-like
            [Amblyomma americanum].
ACCESSION   XP_077510451
VERSION     XP_077510451.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654325.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..265
                     /product="microtubule-associated protein RP/EB family
                     member 1-like"
                     /calculated_mol_wt=30054
     Region          29..>95
                     /region_name="CH_SF"
                     /note="calponin homology (CH) domain superfamily; cl00030"
                     /db_xref="CDD:469584"
     CDS             1..265
                     /gene="LOC144121225"
                     /coded_by="XM_077654325.1:1..798"
                     /db_xref="GeneID:144121225"
ORIGIN      
        1 mdlekrprrs sacpssavrf ttgrmgvlaw inrtlgydik dlnelasgta ycelvnhafp
       61 gtirktliky gdnlkkheke qhfrtlrrsl srvgedveln ieklvnggye ehlnfarwim
      121 efcerrriks seavddvqec avssseangg apkeaaapss eanvdapkek lwdekenqdp
      181 gesessvfmk katffrqere ktlkfitrqr nayfdylmdi gkicrtakkq glkskwldqi
      241 eeiieaslrv pltpdselsg etpsk
//
LOCUS       XP_077510464             265 aa            linear   INV 25-NOV-2025
DEFINITION  microtubule-associated protein RP/EB family member 1-like
            [Amblyomma americanum].
ACCESSION   XP_077510464
VERSION     XP_077510464.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654338.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..265
                     /product="microtubule-associated protein RP/EB family
                     member 1-like"
                     /calculated_mol_wt=30054
     Region          29..>95
                     /region_name="CH_SF"
                     /note="calponin homology (CH) domain superfamily; cl00030"
                     /db_xref="CDD:469584"
     CDS             1..265
                     /gene="LOC144121232"
                     /coded_by="XM_077654338.1:1..798"
                     /db_xref="GeneID:144121232"
ORIGIN      
        1 mdlekrprrs sacpssavrf ttgrmgvlaw inrtlgydik dlnelasgta ycelvnhafp
       61 gtirktliky gdnlkkheke qhfrtlrrsl srvgedveln ieklvnggye ehlnfarwim
      121 efcerrriks seavddvqec avssseangg apkeaaapss eanvdapkek lwdekenqdp
      181 gesessvfmk katffrqere ktlkfitrqr nayfdylmdi gkicrtakkq glkskwldqi
      241 eeiieaslrv pltpdselsg etpsk
//
LOCUS       XP_077510497             391 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121254 [Amblyomma americanum].
ACCESSION   XP_077510497
VERSION     XP_077510497.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654371.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..391
                     /product="uncharacterized protein LOC144121254"
                     /calculated_mol_wt=41762
     Region          <1..102
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            22
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238827"
     Region          164..260
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     Site            order(167..170,200..201,204,238,256)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:259998"
     Region          <321..350
                     /region_name="HybF"
                     /note="Hydrogenase maturation factor HypA/HybF,
                     metallochaperone involved in Ni insertion
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG0375"
                     /db_xref="CDD:440144"
     CDS             1..391
                     /gene="LOC144121254"
                     /coded_by="XM_077654371.1:1..1176"
                     /db_xref="GeneID:144121254"
ORIGIN      
        1 mqvrvggtls spppittgvt kgsvlspflf nlvmaglpsv iqqglchrvf csvyvddval
       61 lvlasrrhia sarqalqral devqcfqasr dlvlaatkse alfvlprgsr atsrirtlri
      121 cgsdlpleai sldfggtskr hkpaaalnqp atafldaike ptllftdgsv lpatrkgaat
      181 lvapqlkatr trklpfpgss taaelaglhl vadliatips gpacrpstda ghqiflhlep
      241 ghsgiegndl adtlaksaht dgspivralh rdrrtaaykp frqlsdqlpr rgralllrlr
      301 igctwtpsrl yacgrghtpa cpscgytgtl ghlllacpth dsprlrlesg yralglpptm
      361 khdllfpahs qllahrvlls fleetglsil l
//
LOCUS       XP_077510518             262 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121269 [Amblyomma americanum].
ACCESSION   XP_077510518
VERSION     XP_077510518.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654392.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..262
                     /product="uncharacterized protein LOC144121269"
                     /calculated_mol_wt=29666
     Region          9..59
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          88..248
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..262
                     /gene="LOC144121269"
                     /coded_by="XM_077654392.1:58..846"
                     /db_xref="GeneID:144121269"
ORIGIN      
        1 mlmqgkrfcl snedavlltl iklhhnmsfa llgvlfgihr ttaseifkas vrilaeimkn
       61 avfwpskeav vdnlmvyfke yssvravldc teisiqrpkd mesqpltysw ykgvytakvl
      121 vcetpgghis yvskayggra sdshitkesk ilelflpsiy svmldkgflt dklciehqit
      181 mvrppflrkr kqltknehek nqsiagarih leraiermkv fkiftgnlgt elfayiddir
      241 eviagivnls kpifcsdnvl vq
//
LOCUS       XP_077510527             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121279 [Amblyomma americanum].
ACCESSION   XP_077510527
VERSION     XP_077510527.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654401.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144121279"
                     /calculated_mol_wt=17707
     Region          <9..117
                     /region_name="Lipase_GDSL_2"
                     /note="GDSL-like Lipase/Acylhydrolase family; pfam13472"
                     /db_xref="CDD:463889"
     CDS             1..158
                     /gene="LOC144121279"
                     /coded_by="XM_077654401.1:135..611"
                     /db_xref="GeneID:144121279"
ORIGIN      
        1 miretcfrha dfvalyvggn dldrgddpre iashikdlvl llqelvasvv lvfkvlprhc
       61 ddphkarfed rrrcqlnrrl tatmkrlsgv hvlnpehrfl dasgkpmlsl faadryhvar
      121 grgidqmski ivaalvkiyg pgiasiqgqd qerlaspe
//
LOCUS       XP_077510576             203 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121303 [Amblyomma americanum].
ACCESSION   XP_077510576
VERSION     XP_077510576.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654450.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..203
                     /product="uncharacterized protein LOC144121303"
                     /calculated_mol_wt=21272
     CDS             1..203
                     /gene="LOC144121303"
                     /coded_by="XM_077654450.1:51..662"
                     /db_xref="GeneID:144121303"
ORIGIN      
        1 mdsaslksap fgpsvgsspa sprfptppcs rgaaeraqgs ypedcgrnvd sakrslaghp
       61 sssstaafsr sdpaianted gevvpaaaae lhvdvrrgcc tgehpagprr qhvihllpwc
      121 fdnegltang pgsaprhtwt atsvlgrpri plrgrhcllg rapplspprl rgcwqclsee
      181 vvaerargtr rtaisaafph ppv
//
LOCUS       XP_077510589             203 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121309 [Amblyomma americanum].
ACCESSION   XP_077510589
VERSION     XP_077510589.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654463.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..203
                     /product="uncharacterized protein LOC144121309"
                     /calculated_mol_wt=21132
     CDS             1..203
                     /gene="LOC144121309"
                     /coded_by="XM_077654463.1:53..664"
                     /db_xref="GeneID:144121309"
ORIGIN      
        1 mdsaslksap fgptvgsspa sprsptppcs rtaaeraqgs ypedcgrnvd sakrslaghp
       61 sssstaafsr sdpaianted gevvpaaaae lhvdvrrgcc pgerpagprr qhvthllpwc
      121 fdnegltasv pgsaprhtwt atsvlgrpri plrgrncrlg rapapspprl rgswqcfsge
      181 vvaerargtr gtaisaafpr ppv
//
LOCUS       XP_077510602             245 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121315 [Amblyomma americanum].
ACCESSION   XP_077510602
VERSION     XP_077510602.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654476.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..245
                     /product="uncharacterized protein LOC144121315"
                     /calculated_mol_wt=26132
     CDS             1..245
                     /gene="LOC144121315"
                     /coded_by="XM_077654476.1:34..771"
                     /db_xref="GeneID:144121315"
ORIGIN      
        1 mpiilssavs ghaqflfedr pawsfcrsdv clatppnssv lacaaekslg fclessgrnm
       61 dsakrapgrp pvselwqgrg schgncgryv nsakrppvse lqqtlvvwvr sydsghgdqg
      121 ggdghcqrdp rqrpprlhar vsaqpgravs rpsihhhral tttayppapr mrslanaerd
      181 lclglpvypp mreallsapl tasaatttkv lavlrpeerg gkrtrdpaha dlgcfspssa
      241 vsgis
//
LOCUS       XP_077510634             397 aa            linear   INV 25-NOV-2025
DEFINITION  protein ABHD1-like [Amblyomma americanum].
ACCESSION   XP_077510634
VERSION     XP_077510634.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654508.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..397
                     /product="protein ABHD1-like"
                     /calculated_mol_wt=44041
     Region          61..376
                     /region_name="YheT"
                     /note="Predicted hydrolase of the alpha/beta-hydrolase
                     fold [General function prediction only]; COG0429"
                     /db_xref="CDD:440198"
     CDS             1..397
                     /gene="LOC144121336"
                     /coded_by="XM_077654508.1:1..1194"
                     /db_xref="GeneID:144121336"
ORIGIN      
        1 mgvaqhlkap tkgelllcla csayvvyyvl vvvrkprvvc rtghvrrflc vqmegfvkgy
       61 frtpvwciga nlqrlvgyly hwwlprvnyr rellvlsdrg lvaldwlnee argpvvlvlv
      121 aggytdsqsr pwrsllpala aldfpcvvvn grgcggvplt thrityaasv sdfaevvkkv
      181 rqryphecll gvgvslggll vslylcqrgr qaqldaalav sppfqpamaa ralanpwgfn
      241 qllnacitrg lvgrlrdned vvrasrvira ddvfrcwtla sfdrhyaapa fgfqsvqdfy
      301 encslkgklc mvqrplvflv seddslnpet alpedevlks pwlgavvtpr gghmgfvdgw
      361 llprqpfyae rfvvafavgl rkvckargvr vlhtlgn
//
LOCUS       XP_077510648             397 aa            linear   INV 25-NOV-2025
DEFINITION  protein ABHD1-like [Amblyomma americanum].
ACCESSION   XP_077510648
VERSION     XP_077510648.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654522.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..397
                     /product="protein ABHD1-like"
                     /calculated_mol_wt=44138
     Region          61..376
                     /region_name="YheT"
                     /note="Predicted hydrolase of the alpha/beta-hydrolase
                     fold [General function prediction only]; COG0429"
                     /db_xref="CDD:440198"
     CDS             1..397
                     /gene="LOC144121339"
                     /coded_by="XM_077654522.1:1..1194"
                     /db_xref="GeneID:144121339"
ORIGIN      
        1 mgvvphanaa tkaglllcla csayvvyyvl vvvrkprvvc rtghvrrflc vqmegfvkgy
       61 frtpvwciga dlqrlvgcly hwwlprvnyr rellvlsdrg lvaldwlnee argpvvlvlv
      121 aggytdsqsr pwrsllpala aldfpcvvvn grgcggeplt thritnaasv rdfaevvkkv
      181 rqryprkcll gvgvslggll lcrylcqrgp raqldaalav sppfqpvvaa ralanpwgfn
      241 rllnacitrg lvgrlrdned vvrasraira dhlfrcwtla sfyrryaaps frfqslpdfy
      301 encslkgklc mvqrplvflv seddslnpet alpedevlks pwlgavvtpr gghmgfvdgw
      361 llprqpfyae rfvvafavgl rkvckargvr vlqtlgd
//
LOCUS       XP_077510660             327 aa            linear   INV 25-NOV-2025
DEFINITION  protein ABHD1-like [Amblyomma americanum].
ACCESSION   XP_077510660
VERSION     XP_077510660.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654534.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..327
                     /product="protein ABHD1-like"
                     /calculated_mol_wt=36010
     Region          <55..306
                     /region_name="YheT"
                     /note="Predicted hydrolase of the alpha/beta-hydrolase
                     fold [General function prediction only]; COG0429"
                     /db_xref="CDD:440198"
     CDS             1..327
                     /gene="LOC144121348"
                     /coded_by="XM_077654534.1:1..984"
                     /db_xref="GeneID:144121348"
ORIGIN      
        1 mlcrtghvrr flcvkmegfv kgyfrtpvwc iganlqrtfv svsgrgsgrs rarqrgflst
       61 lyaenshpla tlgfpcvvvn ghgcggvplt thrvtyaasv rdfaevvkkv rqryphecll
      121 gvgvslggll vslylcqrgr qaqldaalav sppfqpamaa ralanpwgfn rllnacitrg
      181 lvgrlrdned vvrasrvira ddvfrcwtla sfdrhyaapa fgfqsvqdfy encslkgklc
      241 mvqrplvflv seddalnpet alpedevlks pwlgavvtpr gghmgfvdgw llprqpfyae
      301 rfvvafavgl rkvckargvr vlhtlgn
//
LOCUS       XP_077510671             397 aa            linear   INV 25-NOV-2025
DEFINITION  protein ABHD1-like [Amblyomma americanum].
ACCESSION   XP_077510671
VERSION     XP_077510671.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654545.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..397
                     /product="protein ABHD1-like"
                     /calculated_mol_wt=44143
     Region          61..376
                     /region_name="YheT"
                     /note="Predicted hydrolase of the alpha/beta-hydrolase
                     fold [General function prediction only]; COG0429"
                     /db_xref="CDD:440198"
     CDS             1..397
                     /gene="LOC144121355"
                     /coded_by="XM_077654545.1:1..1194"
                     /db_xref="GeneID:144121355"
ORIGIN      
        1 mgvvphanaa tkaglllcla csayvvyyvl vvvrkprvvc rtghvrrflc vqmegfvkgy
       61 frtpvwciga dlqrlvgcly hwwlprvnyr rellvlsdrg lvaldwlnee argpvvlvlv
      121 aggytdsqsr pwrsllpala aldfpcvvvn grgcggeplt thritnaasv rdfaevvkkv
      181 rqryprtcll gvgmslggll lcrylcqrgp raqldaalav sppfqpvvaa ralanpwgfn
      241 rllnacitrg lvgrlrdned vvrasraira dhlfrcwtla sfyrryaaps frfqslpdfy
      301 encslkgklc mvqrplvflv seddslnpet alpedevlks pwlgavvtpr gghmgfvdgw
      361 llprqpfyae rfvvafavgl rkvckargvr vlqtlgd
//
LOCUS       XP_077510681             327 aa            linear   INV 25-NOV-2025
DEFINITION  protein ABHD1-like [Amblyomma americanum].
ACCESSION   XP_077510681
VERSION     XP_077510681.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654555.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..327
                     /product="protein ABHD1-like"
                     /calculated_mol_wt=35960
     Region          <55..306
                     /region_name="YheT"
                     /note="Predicted hydrolase of the alpha/beta-hydrolase
                     fold [General function prediction only]; COG0429"
                     /db_xref="CDD:440198"
     CDS             1..327
                     /gene="LOC144121363"
                     /coded_by="XM_077654555.1:1..984"
                     /db_xref="GeneID:144121363"
ORIGIN      
        1 mlcrtghvrr flcvkmegfv kgyfrtpvwc iganlqrtfv svsgrgsgrs rarqrgflst
       61 lyaenshpla tlgfpcvvvn ghgcggvplt thrvtyaasv rdfaevvkkv ckryprecll
      121 vvgvslggll vslylcqrgr qaqldaalav sppfqpavaa ralanpwgfn rllnacitrg
      181 lvgrlrdned vvrasrvira ddvfrcstla sfdrhyaasa fgfqsvlhfy enyslkgklc
      241 mvqrplvflv seddslnpet alpedevlks pwlgavvtpr gghmgfvdgw llprqpfyae
      301 rfvvafavgl rkvckargvr vlhtlgn
//
LOCUS       XP_077510692             278 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121373 [Amblyomma americanum].
ACCESSION   XP_077510692
VERSION     XP_077510692.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654566.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..278
                     /product="uncharacterized protein LOC144121373"
                     /calculated_mol_wt=29868
     CDS             1..278
                     /gene="LOC144121373"
                     /coded_by="XM_077654566.1:104..940"
                     /db_xref="GeneID:144121373"
ORIGIN      
        1 maehgngesg acggsgdagg gdhskearre aaerarlaar eqlrqisekr rllaeasyde
       61 gvemaleiva rdrnygakfs cgslraanrc arlgfpgsri rlflraagas elqrrerava
      121 vyldtvarhs nlslrlplrf hdteyaglvg gdgsvladfe aeswdqecdd pllvvedidd
      181 sedeeteepd dpampadpam padpgmaama amaampanpa kpgreaaate avaemesdsd
      241 tdtesdsdte veddisdrei deeqayeady svesdddf
//
LOCUS       XP_077510731             155 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121409 [Amblyomma americanum].
ACCESSION   XP_077510731
VERSION     XP_077510731.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654605.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..155
                     /product="uncharacterized protein LOC144121409"
                     /calculated_mol_wt=17112
     Region          9..84
                     /region_name="THAP"
                     /note="The THAP domain is a putative DNA-binding domain
                     (DBD) and probably also binds a zinc ion; smart00980"
                     /db_xref="CDD:214951"
     CDS             1..155
                     /gene="LOC144121409"
                     /coded_by="XM_077654605.1:1..468"
                     /db_xref="GeneID:144121409"
ORIGIN      
        1 mslwhvkkrc svvhcdsvds tigvtlhrfp ldfhrcrawa qfcrnvslek kapsqlrelr
       61 icsrhfepsv yltsgrlrhd alptrasiad tssecssdmd csqslsetsa svrragepaa
      121 vsgepvskgy vqsrssqsll lfqegdcyfv sarta
//
LOCUS       XP_077510737             155 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121421 [Amblyomma americanum].
ACCESSION   XP_077510737
VERSION     XP_077510737.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654611.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..155
                     /product="uncharacterized protein LOC144121421"
                     /calculated_mol_wt=17112
     Region          9..84
                     /region_name="THAP"
                     /note="The THAP domain is a putative DNA-binding domain
                     (DBD) and probably also binds a zinc ion; smart00980"
                     /db_xref="CDD:214951"
     CDS             1..155
                     /gene="LOC144121421"
                     /coded_by="XM_077654611.1:1..468"
                     /db_xref="GeneID:144121421"
ORIGIN      
        1 mslwhvkkrc svvhcdsvds tigvtlhrfp ldfhrcrawa qfcrnvslek kapsqlrelr
       61 icsrhfepsv yltsgrlrhd alptrasiad tssecssdmd csqslsetsa svrragepaa
      121 vsgepvskgy vqsrssqsll lfqegdcyfv sarta
//
LOCUS       XP_077510783             312 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121452 [Amblyomma americanum].
ACCESSION   XP_077510783
VERSION     XP_077510783.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654657.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..312
                     /product="uncharacterized protein LOC144121452"
                     /calculated_mol_wt=34227
     Region          17..75
                     /region_name="DM3"
                     /note="Zinc finger domain in CG10631, C. elegans LIN-15B
                     and human P52rIPK; smart00692"
                     /db_xref="CDD:128933"
     CDS             1..312
                     /gene="LOC144121452"
                     /coded_by="XM_077654657.1:1..939"
                     /db_xref="GeneID:144121452"
ORIGIN      
        1 mlrvhcnsvd sttgvklhrf pldfhrcraw aqfcrnvsle kkapsqlrel ricsrhfeps
       61 vyltsgrlrh dalptrasia dtssecssdm ncsqslsets asvrragepa avsgepvskr
      121 yvqsrssqll llfqedadga sptgstlnqd cshaigfimv qsdhtyagps teasasslep
      181 gmagvaatls astdlspgap fsssplsyeg sftspinerr plqklraklr qlrnrnkrlq
      241 glllqrrrmk ttaelvdslr ehltpavaaf veaqlkmhnv srfgrrwcsq nktfalglyf
      301 hsqkvtdiag ns
//
LOCUS       XP_077510796             400 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121459 [Amblyomma americanum].
ACCESSION   XP_077510796
VERSION     XP_077510796.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654670.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..400
                     /product="uncharacterized protein LOC144121459"
                     /calculated_mol_wt=45192
     Region          183..345
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..400
                     /gene="LOC144121459"
                     /coded_by="XM_077654670.1:1..1203"
                     /db_xref="GeneID:144121459"
ORIGIN      
        1 mvvaadleve ieaaksqtds mkrrllfang lciaaavacd gtvqrerlmy nrnerwfedt
       61 lphlgerffk qsfrvtpptf ryiveacrpl lqrvdtnmrl aisvenraav amyklcssae
      121 drsvanlfgl grstvniiyr efcdavvcll edkwinmpsp damgdhiref gtvcdfpqai
      181 galdgchfpv sppkesapdy ynykgwhsiv llalvdhryr fryvnvgspg rchdayiyqr
      241 sqlaefiegp tfqapavvfs gtavpplilc dqafpltshl ikpfshsayl dadmkrlnyh
      301 lskarrvven afgrlkarfr fcmkrmecsi dtaplvirac ctlnniceaf gdqvmqdwts
      361 daqvsealyq qpqrvtddqt atgsavraai leyykqraqn
//
LOCUS       XP_077510822             400 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121470 [Amblyomma americanum].
ACCESSION   XP_077510822
VERSION     XP_077510822.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654696.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 11% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..400
                     /product="uncharacterized protein LOC144121470"
                     /calculated_mol_wt=44035
     CDS             1..400
                     /gene="LOC144121470"
                     /coded_by="XM_077654696.1:113..1315"
                     /db_xref="GeneID:144121470"
ORIGIN      
        1 mdvpvmtdvc qggsphastp cmelgvtesl dalqtglggn yedcgegrgl vpkaviicpq
       61 trqeglsgvd skkevalwal qkrccmvrde rsnrteeryg nimrlggkms lrfdgavtpt
      121 vlasaipfvg lpllvflgvm mrhrqstlpl ltdvkvpali vieslifvgp egvlhlclea
      181 lhkgrhsasf avlvltrlly tvwlvvsfrv ngpllasgla mmrgrfamav flyaydgysk
      241 ylvclprtls ylygsetctl ptwcrkpsrr skedifeaca clyemlekse haeviqmhne
      301 kygphmrnfv rdntvgacvw vcalclavlv ilrmfkliwg diaavvflck rytaerrkgf
      361 trndafqaae lallatdtsp mkpllaagss vvgiitkkii
//
LOCUS       XP_077510832             247 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121478 [Amblyomma americanum].
ACCESSION   XP_077510832
VERSION     XP_077510832.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654706.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 23% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..247
                     /product="uncharacterized protein LOC144121478"
                     /calculated_mol_wt=28076
     CDS             1..247
                     /gene="LOC144121478"
                     /coded_by="XM_077654706.1:51..794"
                     /db_xref="GeneID:144121478"
ORIGIN      
        1 mrqasgldat fegfkgpspl lrlkgldlvw glppdymhcv legvtkqlve qwlsvtgtaf
       61 yigrhvrlld srlctikppi nfsriarpll eraywkatew rywllfysvp cfhsfpclsd
      121 llprpymvhf allvkalfll lqdtisesdi staevlllsf vqqvarlygd tamtfnvhql
      181 lhlpkatrmf gplwglstfp fedeigrtlr lvsaaklvpm qvaertqtat ehvlsvcrtl
      241 fqnflst
//
LOCUS       XP_077510855             695 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121495 [Amblyomma americanum].
ACCESSION   XP_077510855
VERSION     XP_077510855.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654729.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..695
                     /product="uncharacterized protein LOC144121495"
                     /calculated_mol_wt=77859
     Region          237..383
                     /region_name="Transposase_21"
                     /note="Transposase family tnp2; cl29371"
                     /db_xref="CDD:475188"
     CDS             1..695
                     /gene="LOC144121495"
                     /coded_by="XM_077654729.1:1..2088"
                     /db_xref="GeneID:144121495"
ORIGIN      
        1 msmekevmsk wrryriieke yrellgelgi rrppsthlks sgskrtggea aldvvsappl
       61 plpsgaskrp nseacasgac asgsgraiss lpadpsmpsv qselneqayv sescaeslss
      121 ehtptscdss detsstnnlt aenvemgqpr eespelcyrh lstaqelasi askhnlthac
      181 indlldfcrr rgivelpkda rtimqterka nlehgdsfvh fglaegiqha vgqgpapenv
      241 elhanidglp lhrssqtalw pilcrvinik ksepfmvsay cgaekppclq eylrpfidev
      301 rnlscnglmv kgvhvnvclk avicdapars fvkaitghtg yhacercsqk ghylenrmtf
      361 pdlyapqrtd ssfrsqedrr hhpgvspfts lnvdmvkcfp teymhlvclg vmrlllrnwi
      421 ckghaarlsr adrsalndkl qeaatafpky fqrkprgtee ldrwkatefr tfllylgpvl
      481 lksilpdely khflylhvsv rvlaspqhhr dlnqfahdll ryfvqefgrl ygqkqlvynv
      541 htlahlaeqc lehgtvdefs afsfesylgk akkrlrtink plaqlsrrms evractplst
      601 gqvggdvkvg dcflvdncav vivdllrgna kagilpngre ffrvpidssr mdvrrysalg
      661 qetkiwpisy iknkvrcikl qykrghvvfp llqlq
//
LOCUS       XP_077510890             321 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121521 [Amblyomma americanum].
ACCESSION   XP_077510890
VERSION     XP_077510890.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654764.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 47% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..321
                     /product="uncharacterized protein LOC144121521"
                     /calculated_mol_wt=34939
     Region          <89..142
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     CDS             1..321
                     /gene="LOC144121521"
                     /coded_by="XM_077654764.1:1..966"
                     /db_xref="GeneID:144121521"
ORIGIN      
        1 mafgsriref dlsensswvd yverielsca anklttdddk ravllsccgp etysliatlv
       61 klsrppnvgy qvivdavkkh inpkpselys rtmdtvlagm phvatylddi liasesieeh
      121 ermlaevlsr laksglgvqa ekrrstrasg prgrcqgrsp vvvvpsrfsl calllpgcai
      181 cilrhfaflc varwkaaktq sknvwaaaen qlqrvkhaae ggleglpttl hlavigssmg
      241 dikkiivavd svefistrsl ekamglllga ffvfkvaypp dcpltmeflq rylgqinptk
      301 grgkgracgi apkllsllqa l
//
LOCUS       XP_077511008             365 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121592 [Amblyomma americanum].
ACCESSION   XP_077511008
VERSION     XP_077511008.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654882.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..365
                     /product="uncharacterized protein LOC144121592"
                     /calculated_mol_wt=40626
     CDS             1..365
                     /gene="LOC144121592"
                     /coded_by="XM_077654882.1:1..1098"
                     /db_xref="GeneID:144121592"
ORIGIN      
        1 mdafdvllya nsvaantqeq rqqnqetkfr lllgrqteet atscvhnlss ykldhaelsl
       61 lnkglnfntg pvpdaksmtc avelavrkid aslryqarsr aigalsklrk ekdsalprpe
      121 kealkrlqkn eavvilpadk gnatvvldrt eyikkmsall edkntytkin rdptrkieae
      181 lqklladvfk yvppsdgqyi ynkllchnga apaiygvpkv hkkgtplrpi vdftrsplnk
      241 lsgylhrvla plagktathv rnasdfvekl knvpiddtqv masfdvkslf tsvpvdfave
      301 cckkalnedt tlpertpiea ddlcrllkfc lsntyftfnk nfyrqtfgta mgasvsvave
      361 aqehd
//
LOCUS       XP_077511021             556 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121596 [Amblyomma americanum].
ACCESSION   XP_077511021
VERSION     XP_077511021.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654895.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 12% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..556
                     /product="uncharacterized protein LOC144121596"
                     /calculated_mol_wt=59675
     CDS             1..556
                     /gene="LOC144121596"
                     /coded_by="XM_077654895.1:1..1671"
                     /db_xref="GeneID:144121596"
ORIGIN      
        1 mrlvhqisgg ggrceratqs pdggvrrrpg rrfvgrnvna pakratgskd kafskwkprp
       61 mpkptpedyi ivikpresvs lheaftetgy gtaisaylgl kraraisvlp sreqnliivh
      121 tpdieaadql igdfavnsdn gsiplrgylr qdggnichgv ivvrnsdtse tlkdrvcwht
      181 gtileirkfg tsnkaritfa gkekpryvhy dnmvvsvrny yktipacgqc gvvghradac
      241 pnlrsntcgl cgfqtplveg vraphncvpr cslcggshat nsrdcavkfr tpktkkkkta
      301 skkksrylgp pgdqhgqkis ntenpptgga akqpskvspp hgglakqste trgdtkarvn
      361 avknghqvsg sggaaslspl stlfprahsa eqdqiaalra qnemllkkin eleskinqss
      421 sppsppaaev meselvvpka vtsleaafea rlgatearfa aienqismmi naisklqeti
      481 pamiaqqian slrssrrpgd plpgrphkts rrtpevaddd ncslsgmedt pllasvgsga
      541 vsvqpllnln dhgeqp
//
LOCUS       XP_077511040             263 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121607 [Amblyomma americanum].
ACCESSION   XP_077511040
VERSION     XP_077511040.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654914.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..263
                     /product="uncharacterized protein LOC144121607"
                     /calculated_mol_wt=28863
     CDS             1..263
                     /gene="LOC144121607"
                     /coded_by="XM_077654914.1:1..792"
                     /db_xref="GeneID:144121607"
ORIGIN      
        1 mapagrsrsp ttpttppfvi mapprdpgtf sgtdgsdved winlyervgn ynrgdptlml
       61 anvifslngt arvwyethee eltswdtfke klktlfgrpd drqlaakkql atraqsstes
      121 yvayiqdila lchkvdaams etdkvghvlk giaddafhll vckdctsids iinecrrfeq
      181 vkgrrispsf srlpytaats scedhrspsr tpsdapavtd svtrivrrel ealapvispp
      241 svpdttlpav sliqavvrqd sli
//
LOCUS       XP_077511050             737 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121615 [Amblyomma americanum].
ACCESSION   XP_077511050
VERSION     XP_077511050.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654924.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 23% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..737
                     /product="uncharacterized protein LOC144121615"
                     /calculated_mol_wt=82577
     Region          97..>507
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..737
                     /gene="LOC144121615"
                     /coded_by="XM_077654924.1:1..2214"
                     /db_xref="GeneID:144121615"
ORIGIN      
        1 mpwntrsgps slptkracps sphscggsaa crsqpadvss lrsvpryavf vagvafiasv
       61 cllvvpaaiv snaasceglg kclslekdll rsldksvhpc ddfyqhvcgg wddpvsghdk
      121 spserysghf kdnflwnmlr rkipqrplta adkvsfmlls clkqlgrdgs delraflrdl
      181 glpwphrspa traqllqslv klsltlgmpt fwafmigrhp arpsqntlym tmdrrllews
      241 hdvdklhshg alhqylrrca evlggtgqsy slmianvlea henisdivqe hwqlfcipry
      301 dnlsdaelrr avnghlpdds qlwpedeivn lqptlfdvld rfhltpdenr erfklflgay
      361 lvwtvaplvt sqlavamlgd mgyrdspvsf msrkcfssvs fvlplvawkl qtnsvqskhl
      421 lwsvfqvvka allkalgsyr eaiavranhv asrllvnafn msatwatvdn iyafldmggp
      481 dsisyfhmfr hvsrdtvtly kqslrkprht mfyvpgvare piyrllvtre vtvpnhwtal
      541 altgsthslp vmaavlgmgq vaqmlallnf vffydeqftr ypyeelkddf ggwlahlkrf
      601 ervlekhsll pdlttsdkrq lmrlslsalv gahasarpgr dtvgtapefa saqrgqwsts
      661 gpaslhgipk eelfflvscf ldcglkgrqr llqqtycnva lpsvpafgst fqcneshrmk
      721 tnftwqkvdd dinvdds
//
LOCUS       XP_077511060             293 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121624 [Amblyomma americanum].
ACCESSION   XP_077511060
VERSION     XP_077511060.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654934.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..293
                     /product="uncharacterized protein LOC144121624"
                     /calculated_mol_wt=33171
     Region          86..240
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..293
                     /gene="LOC144121624"
                     /coded_by="XM_077654934.1:1..882"
                     /db_xref="GeneID:144121624"
ORIGIN      
        1 mrkpisaekh vavglhrlcs naedrtvahl fgigrstvdi vyrefcgavv dvlegawlhm
       61 qreeemvehi recyavtgfp qaigaidgch caisppkkda adycnyngwy sryhyrflyl
      121 nvrspsrchd ahvygrsrln kyvesdnfks lvfvieatpv ariilcdqaf pvtphllkpy
      181 pnatsgskqa ifndnlsksr rivgnafgra karfkfiikr meskllnakq virasctlhn
      241 icetfqdvee qwildahtyn alyeqpshnt eacagegqdv raalaeylwk qaq
//
LOCUS       XP_077511083             257 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121642 [Amblyomma americanum].
ACCESSION   XP_077511083
VERSION     XP_077511083.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654957.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..257
                     /product="uncharacterized protein LOC144121642"
                     /calculated_mol_wt=28518
     CDS             1..257
                     /gene="LOC144121642"
                     /coded_by="XM_077654957.1:1..774"
                     /db_xref="GeneID:144121642"
ORIGIN      
        1 mhpihhkerr ekraealekr fknskdvlyv daadygngrm alaigeqsai alevastsak
       61 iivgasevav mnfarsrisp eanrilqtcr dsrkfeiiwa pahaflagnk aahelarglt
      121 lragglaegf tgrdrllcyr eitqhyrlgr vkyppadisl nksesstwrr lqtnsfpspv
      181 scslyypgqy ssqcklckar adrnhilwec pqapsegsik sleqwetlll gsdpeiqlkt
      241 vrlaetaarv qgllavv
//
LOCUS       XP_077511106             106 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121651 [Amblyomma americanum].
ACCESSION   XP_077511106
VERSION     XP_077511106.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077654980.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..106
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..106
                     /product="uncharacterized protein LOC144121651"
                     /calculated_mol_wt=10701
     CDS             1..106
                     /gene="LOC144121651"
                     /coded_by="XM_077654980.1:85..405"
                     /db_xref="GeneID:144121651"
ORIGIN      
        1 mdensgdkap ppvgrargrs rdrartvvvl pgaqaaptvp qfpaamapla alprepgvlr
       61 patvtlpsmp thdsvaglss sfdrmaigqg ahlqqtaama psavsi
//
LOCUS       XP_077511128             119 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121666 [Amblyomma americanum].
ACCESSION   XP_077511128
VERSION     XP_077511128.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655002.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..119
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..119
                     /product="uncharacterized protein LOC144121666"
                     /calculated_mol_wt=12792
     CDS             1..119
                     /gene="LOC144121666"
                     /coded_by="XM_077655002.1:1..360"
                     /db_xref="GeneID:144121666"
ORIGIN      
        1 mlsadttfal cspgieigqs ptfltrpdyl ippafeqpta appvarmedf gqrtpsmqnf
       61 sggaigytps rratammast dlsqqrghrt yahddqpasr ldvaqatlaf ghyhapirl
//
LOCUS       XP_077511140             547 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121674 [Amblyomma americanum].
ACCESSION   XP_077511140
VERSION     XP_077511140.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655014.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 28% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..547
                     /product="uncharacterized protein LOC144121674"
                     /calculated_mol_wt=60237
     CDS             1..547
                     /gene="LOC144121674"
                     /coded_by="XM_077655014.1:1..1644"
                     /db_xref="GeneID:144121674"
ORIGIN      
        1 mdgdvtkkrr kryfykdepf vvpkttlyrr qqevrlraqh casstsaasa dvdvegddeg
       61 dvngggvvgd llnllsgeqp aadqaaqpas ptlsahdeqa ggssyadded lfrtddfdrl
      121 getpedsssm sgetesddge rdflasdfve leaailpgst ttkaaaiimi mafvvthglt
      181 sealndllrl idglfgfkgd vlprskfvfr klwssrkerl vkrffycdtc gsvlvsvpgm
      241 ssmrcpdcev stelcvlkar ghffiildmk eqmkcllaks kaalferlvn lkaviqqgga
      301 allqditsgs maeelrksgk igsmdltiti ntegspvfks ssssvwpiqf linelppcdr
      361 lkncligglw fgqhpymrtf ltkfveeinq fgkitwkagh tllssglhvl cccvdapara
      421 svinvvqfng lfgcpwcync aefhewaqry msvtvgeert pgemskdmef aeikdavngl
      481 kgqsplnhlk hfnivfghtv eymhcvhigv tkcltdqwfd sansqqpfyi aasaifsasl
      541 cefvcsm
//
LOCUS       XP_077511152             392 aa            linear   INV 25-NOV-2025
DEFINITION  degenerin mec-4-like [Amblyomma americanum].
ACCESSION   XP_077511152
VERSION     XP_077511152.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655026.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 53% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..392
                     /product="degenerin mec-4-like"
                     /calculated_mol_wt=45089
     Region          10..358
                     /region_name="ASC"
                     /note="Amiloride-sensitive sodium channel; pfam00858"
                     /db_xref="CDD:459966"
     CDS             1..392
                     /gene="LOC144121680"
                     /coded_by="XM_077655026.1:1..1179"
                     /db_xref="GeneID:144121680"
ORIGIN      
        1 msasiclrvf iwtgclvgfl yqtssmlslf feypfsvtlv edvrknllfp aattlldper
       61 whflrkevea rlkvahlpde lflchmrskv tecasfacna airltyyrhp nemcytiday
      121 qwtnsqhpvq rcespwnyel vlrafwerna tvalhepdal plvvhspysc ppdklssvmi
      181 qpgmtytiav sqekverlpa pyksncidyi srgnqkkyfg yytqdicvqn cemslemkhc
      241 qcvrthhefa atfggqvcdi irddictrkv eslgtyqfcl sqcrvacqet kydvrlsslt
      301 vdksdfgkdm nkfsfiikfs sdtqrvlqyq pnlsiveafg yfggyvgiwl gfsllsvlln
      361 leahmakgfa krlkrvktad eavvvfrlkq rf
//
LOCUS       XP_077511165              94 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121685 [Amblyomma americanum].
ACCESSION   XP_077511165
VERSION     XP_077511165.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655039.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..94
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..94
                     /product="uncharacterized protein LOC144121685"
                     /calculated_mol_wt=11070
     Region          7..91
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     CDS             1..94
                     /gene="LOC144121685"
                     /coded_by="XM_077655039.1:166..450"
                     /db_xref="GeneID:144121685"
ORIGIN      
        1 masfneklvq evkkhtqlwd qysrlhkeag yreaawmeia aalavtveec qtrwrtlrdt
       61 ylkrkkrrra gangqwnvle cdlgflddfl rprt
//
LOCUS       XP_077511171             541 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121692 [Amblyomma americanum].
ACCESSION   XP_077511171
VERSION     XP_077511171.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655045.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 27% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..541
                     /product="uncharacterized protein LOC144121692"
                     /calculated_mol_wt=59486
     CDS             1..541
                     /gene="LOC144121692"
                     /coded_by="XM_077655045.1:1..1626"
                     /db_xref="GeneID:144121692"
ORIGIN      
        1 mdgdvtkkrr kryfykdepf vvpkttlyrk qqevrlraqh casstsaasa andgnvnggg
       61 vvgdllnlss geqpaaeqaa qpasptlsah deqaggssya ddedlfrtdd fdrlgetped
      121 sssmsgeten ddgerdflas dfveleaail pgstttkaaa iimimafvvt hgltwealnd
      181 llrlidglfg fkgdvlprsk fvfrklwssr kerlvkrffy cdtcgsvlvs vpgmssmrcp
      241 dcevstelcv lkarghffii lgmkeqmkcl lakskaalfe rlvnlkaviq qggaallqdi
      301 tsgsmaeelr ksgkigsmdl titintegsp vfksssssvw piqflinelp pcdrlkncli
      361 gglwfgqhpy mrtfltkfve einhfgkitw kaghtvlssg lyalcccvda parasvinmv
      421 qfnglfgcpw cyncaefheg aqrymsvtvg eertpgemsk dmefaeikda vnglkgqspl
      481 nhlkhfnivf ghtveymhcv higvtkcltd qwfdsansqq pfyiaasaif saslcefvcs
      541 m
//
LOCUS       XP_077511180             392 aa            linear   INV 25-NOV-2025
DEFINITION  degenerin mec-4-like [Amblyomma americanum].
ACCESSION   XP_077511180
VERSION     XP_077511180.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655054.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 54% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..392
                     /product="degenerin mec-4-like"
                     /calculated_mol_wt=45017
     Region          10..358
                     /region_name="ASC"
                     /note="Amiloride-sensitive sodium channel; pfam00858"
                     /db_xref="CDD:459966"
     CDS             1..392
                     /gene="LOC144121702"
                     /coded_by="XM_077655054.1:1..1179"
                     /db_xref="GeneID:144121702"
ORIGIN      
        1 msasiclrvf iwtgclvgfl yqtssmlslf feypfsvtlv edvrknllfp aattlldper
       61 whflrkegea rlkvahlpde lflchmrskv tecasfacna airltyyrhp nemcytiday
      121 qwtnsqhpvq rcespwnyel vlrafwerna tvalhepdal plvvhspysc ppdklssvmi
      181 qpgmtytiav sqekverlpa pyksncidyi srgnqkkyfg yytqdicvqn cemslemkhc
      241 qcvrthhefa atfggqvcdi irddictrkv eslgtyqfcl sqcrvacqet kydvrlssla
      301 vdksdfgkdm nkfsfiikfs sdtqrvlqyq pnlsiveafg yfggyvgiwl gfsllsvlln
      361 leahmakgfa krlkrvktad eavvvfrlkq rf
//
LOCUS       XP_077511191             542 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121712 [Amblyomma americanum].
ACCESSION   XP_077511191
VERSION     XP_077511191.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655065.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 27% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..542
                     /product="uncharacterized protein LOC144121712"
                     /calculated_mol_wt=59868
     CDS             1..542
                     /gene="LOC144121712"
                     /coded_by="XM_077655065.1:1..1629"
                     /db_xref="GeneID:144121712"
ORIGIN      
        1 mdgdvtkkrr kryfykdepf vvpkttlyrr qqevrlraqh casstsaasa andgdvnggg
       61 vvgdllnlss geqpaaeqaa qpasptlsah deqaggssya ddedlfrtdd fdrlgetped
      121 sssmsgeten ddgerdflas dfveleaail pgstttkaaa iimimafvvt hdltwkvlnd
      181 llrlidglfg fkgdvlprsk fvfrklwssr kerlvkrffy cdtcgsvlvs vpgmssmrcp
      241 dcevstelcv lkarghffii ldmkeqmkcl lakskaalfe rlvnlkaviq qggaallqdi
      301 tsgsmaeefr ksgkigsmdl titintegsp vfksssssvw piqflinelp pcdrlkncli
      361 gglwfgqhpy mrtfltqfve einqfgkitw kaghtllssv lhvlcccvda parasvinmv
      421 qfnglfgcpw cyncaefheg aqrymsvtvg eertpgemsk dkefaekikd avnglkgqsp
      481 lnhlkhfniv fghtveymhc vhigvtkclt dqwfdsadsq qpfyiaasai fsaslcefvc
      541 sm
//
LOCUS       XP_077511202             392 aa            linear   INV 25-NOV-2025
DEFINITION  degenerin mec-4-like [Amblyomma americanum].
ACCESSION   XP_077511202
VERSION     XP_077511202.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655076.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 53% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..392
                     /product="degenerin mec-4-like"
                     /calculated_mol_wt=45059
     Region          10..358
                     /region_name="ASC"
                     /note="Amiloride-sensitive sodium channel; pfam00858"
                     /db_xref="CDD:459966"
     CDS             1..392
                     /gene="LOC144121716"
                     /coded_by="XM_077655076.1:1..1179"
                     /db_xref="GeneID:144121716"
ORIGIN      
        1 msasiclrvf iwtgclvgfl yqtssmlslf feypfsvtlv edvrknllfp aattlldper
       61 whflrkevea rlkvahlpde lflchmrskv tecasfacna airltyyrhp nemcytiday
      121 qwtnsqhpvq rcespwnyel vlrafwerna tvalhepdal plvvhspysc ppdklssvmi
      181 qpgmtytiav sqekverlpa pyksncidyi srgnqkkyfg yytqdicvqn cemslemkhc
      241 qcvrthhefa atfggqvcdi irddictrkv eslgtyqfcl sqcrvacqet kydvrlssla
      301 vdksdfgkdm nkfsfiikfs sdtqrvlqyq pnlsiveafg yfggyvgiwl gfsllsvlln
      361 leahmakgfa krlkrvktad eavvvfrlkq rf
//
LOCUS       XP_077511216             541 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121725 [Amblyomma americanum].
ACCESSION   XP_077511216
VERSION     XP_077511216.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655090.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 27% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..541
                     /product="uncharacterized protein LOC144121725"
                     /calculated_mol_wt=59477
     CDS             1..541
                     /gene="LOC144121725"
                     /coded_by="XM_077655090.1:1..1626"
                     /db_xref="GeneID:144121725"
ORIGIN      
        1 mdgdvtkkrr kryfykdepf vvpkttlyrk qqevrlraqh casstsaasa andgnvnggg
       61 vvgdllnlss geqpaaeqaa qpasptlsah deqaggssya ddedlfrtdd fdrlgetped
      121 sssmsgeten ddgerdflas dfveleaail pgstttkaaa iimimafvvt hgltwealnd
      181 llrlidglfg fkgdvlprsk fvfrklwssr kerlvkrffy cdtcgsvlvs vpgmssmrcp
      241 dcevstelcv lkarghffii lgmkeqmkcl lakskaalfe rlvnlkaviq qggaallqdi
      301 tsgsmaeelr ksgkigsmdl titintegsp vfksssssvw piqflinelp pcdrlkncli
      361 gglwfgqhpy mrtfltkfve einqfgkitw kaghtvlssg lyalcccvda parasvinmv
      421 qfnglfgcpw cyncaefheg aqrymsvtvg eertpgemsk dmefaeikda vnglkgqspl
      481 nhlkhfnivf ghtveymhcv higvtkcltd qwfdsansqq pfyiaasaif saslcefvcs
      541 m
//
LOCUS       XP_077511227             377 aa            linear   INV 25-NOV-2025
DEFINITION  degenerin mec-4-like [Amblyomma americanum].
ACCESSION   XP_077511227
VERSION     XP_077511227.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655101.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 52% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..377
                     /product="degenerin mec-4-like"
                     /calculated_mol_wt=43438
     Region          10..343
                     /region_name="ASC"
                     /note="Amiloride-sensitive sodium channel; pfam00858"
                     /db_xref="CDD:459966"
     CDS             1..377
                     /gene="LOC144121732"
                     /coded_by="XM_077655101.1:1..1134"
                     /db_xref="GeneID:144121732"
ORIGIN      
        1 msasiclrvf iwtgclvgfl yqtssmlslf feypfsvtlv edvrknllfp aattlldper
       61 whflrkevea rlkvahlpde lflchmrskv tecasfacna airltyyrhp nemcytiday
      121 qwtnsqhpvq rcespwnyel vlrafwerna tvalhepdal plvvhspysc ppdklssekv
      181 erlpapyksn cidyisrgnq kkyfgyytqd icvqncemsl emkhcqcvrt hhefaatfgg
      241 qvcdiirddi ctrkveslgt yqfclsqcrv acqetkydvr lsslavdksd fgkdmnkfsf
      301 iikfssdtqr vlqyqpnlsi veafgyfggy vgiwlgfsll svllnleahm akgfakrlkr
      361 vktadeavvv frlkqrf
//
LOCUS       XP_077511239             542 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121741 [Amblyomma americanum].
ACCESSION   XP_077511239
VERSION     XP_077511239.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655113.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 27% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..542
                     /product="uncharacterized protein LOC144121741"
                     /calculated_mol_wt=59864
     CDS             1..542
                     /gene="LOC144121741"
                     /coded_by="XM_077655113.1:1..1629"
                     /db_xref="GeneID:144121741"
ORIGIN      
        1 mdgdvtkkrr kryfykdepf vvpkttlyrr qqevrlraqh casstsaasa andgdvnggg
       61 vvgdllnlss geqpaaeqaa qpasptlsah deqaggssya ddedlfrtdd fdrlgetped
      121 sssmsgeten ddgerdflas dfveleaail pgstttkaat iimimafvvt hdltwevlnd
      181 llrlidglfg fkgdvlprsk fvfrklwssr kerlvkrffy cdtcgsvlvs vpgmssmrcp
      241 dcevstelcv lkarghffii ldmkeqmkcl lakskaalfe rlvnlkaviq qggaallqdi
      301 tsgsmaeelr ksgqigsmdl titintegsp vfksssssvw piqflinelp pcdrlkncli
      361 gglwfgqhpy mrtfltqfve einqfgkitw kaghtllssv lhvlcccvda parasvinmv
      421 qfnglfgcpw cyncaefheg aqrymsvtvg eertpgemsk dkefaekikd avnglkgqsp
      481 lnhlkhfniv fghtveymhc vhigvtkclt dqwfdsansq qpfyiaasai fsaslcefvc
      541 sm
//
LOCUS       XP_077511247             392 aa            linear   INV 25-NOV-2025
DEFINITION  degenerin mec-4-like [Amblyomma americanum].
ACCESSION   XP_077511247
VERSION     XP_077511247.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655121.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 53% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..392
                     /product="degenerin mec-4-like"
                     /calculated_mol_wt=45059
     Region          10..358
                     /region_name="ASC"
                     /note="Amiloride-sensitive sodium channel; pfam00858"
                     /db_xref="CDD:459966"
     CDS             1..392
                     /gene="LOC144121750"
                     /coded_by="XM_077655121.1:1..1179"
                     /db_xref="GeneID:144121750"
ORIGIN      
        1 msasiclrvf iwtgclvgfl yqtssmlslf feypfsvtlv edvrknllfp aattlldper
       61 whflrkevea rlkvahlpde lflchmrskv tecasfacna airltyyrhp nemcytiday
      121 qwtnsqhpvq rcespwnyel vlrafwerna tvalhepdal plvvhspysc ppdklssvmi
      181 qpgmtytiav sqekverlpa pyksncidyi srgnqkkyfg yytqdicvqn cemslemkhc
      241 qcvrthhefa atfggqvcdi irddictrkv eslgtyqfcl sqcrvacqet kydvrlssla
      301 vdksdfgkdm nkfsfiikfs sdtqrvlqyq pnlsiveafg yfggyvgiwl gfsllsvlln
      361 leahmakgfa krlkrvktad eavvvfrlkq rf
//
LOCUS       XP_077511260             435 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121759 [Amblyomma americanum].
ACCESSION   XP_077511260
VERSION     XP_077511260.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655134.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..435
                     /product="uncharacterized protein LOC144121759"
                     /calculated_mol_wt=49869
     Region          5..104
                     /region_name="SNARE_NTD_STX6_STX10"
                     /note="N-terminal domain of syntaxin-6, syntaxin-10, and
                     similar proteins; cd21443"
                     /db_xref="CDD:410569"
     Region          235..319
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     Region          386..420
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..435
                     /gene="LOC144121759"
                     /coded_by="XM_077655134.1:210..1517"
                     /db_xref="GeneID:144121759"
ORIGIN      
        1 msledpffvv kdeivkaian nktvferwnq yqdpsslpsk edidwttsel rkglrsiewd
       61 ledleetvai veknpkkfki dekeiksrka fieqsrnevk cmkeailesk aknkrmrpss
      121 melfnssrta kytslrneve spvhrlldht qqqqqqrtsq rkksawrrce rmlavpvhvr
      181 fprqlvvalw waersvshrr kdeedggtrl rspiptttis apfhrvrama sfneklvqev
      241 kkhtqlwdqq sklhkeagyr eaawmeiaaa ldvtveecqt rwrtlrdtyl krkkrrraga
      301 ngqwnvlerd lgflddflrp rtrrstlnaa apadvevkqe qpgedaemre aavaaaaapp
      361 lamrpaavmp hrlaaggaaa qelssdpeei fclclaahlr glpprdrsia mmkmlqtlhd
      421 lesgenigef algdp
//
LOCUS       XP_077511268             130 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121765 [Amblyomma americanum].
ACCESSION   XP_077511268
VERSION     XP_077511268.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655142.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 23% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..130
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..130
                     /product="uncharacterized protein LOC144121765"
                     /calculated_mol_wt=13920
     CDS             1..130
                     /gene="LOC144121765"
                     /coded_by="XM_077655142.1:1..393"
                     /db_xref="GeneID:144121765"
ORIGIN      
        1 mvlrpgavpv vqparrvpfs lreplreeln rmekasiikr vdeptewfip gkdllladml
       61 srapalravl estedvdvha vqilssigrr lrttlpecgv ekgapgsqap pvvqgqstaa
      121 taarrhgags
//
LOCUS       XP_077511284             542 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121768 [Amblyomma americanum].
ACCESSION   XP_077511284
VERSION     XP_077511284.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655158.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 27% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..542
                     /product="uncharacterized protein LOC144121768"
                     /calculated_mol_wt=59834
     CDS             1..542
                     /gene="LOC144121768"
                     /coded_by="XM_077655158.1:1..1629"
                     /db_xref="GeneID:144121768"
ORIGIN      
        1 mdgdvtkkrr kryfykdepf vvpkttlyrr qqevrlraqh casstsaasa andgdvnggg
       61 ivgdllnlss geqpaaeqaa qpasptlsah deqaggssya ddedlfrtdd fdrlgetped
      121 sssmsgeten ddgerdflas dfveleaail pgstttkaaa iimimafvvt hdltwevlnd
      181 vlrlidglfg fkgdvlprsk fvfrklwssr kerlvkrffy cdtcgsvlvs vpgmssmrcp
      241 dcevstelcv lkarghffii ldmkeqmkcl lakskaalfe rlvnlkaviq qggaallqdi
      301 tsgsmaeelr ksgqigsmdl titintegsp vfksssssvw piqflinelp pcdrlkncli
      361 gglwfgqhpy mrtfltqfve einqfgkitw kaghtllssv lhvlcccvda parasvinmv
      421 qfnglfgcpw cyncaefheg aqrymsvtvg eertpgemsk dkefaekikd avnglkgqsp
      481 lnhlkhfniv fghtveymhc vhigvtkclt dqwfdsansq qpfyiaasai fsaslcefvc
      541 sm
//
LOCUS       XP_077511295             392 aa            linear   INV 25-NOV-2025
DEFINITION  degenerin mec-4-like [Amblyomma americanum].
ACCESSION   XP_077511295
VERSION     XP_077511295.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655169.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 53% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..392
                     /product="degenerin mec-4-like"
                     /calculated_mol_wt=45059
     Region          10..358
                     /region_name="ASC"
                     /note="Amiloride-sensitive sodium channel; pfam00858"
                     /db_xref="CDD:459966"
     CDS             1..392
                     /gene="LOC144121774"
                     /coded_by="XM_077655169.1:1..1179"
                     /db_xref="GeneID:144121774"
ORIGIN      
        1 msasiclrvf iwtgclvgfl yqtssmlslf feypfsvtlv edvrknllfp aattlldper
       61 whflrkevea rlkvahlpde lflchmrskv tecasfacna airltyyrhp nemcytiday
      121 qwtnsqhpvq rcespwnyel vlrafwerna tvalhepdal plvvhspysc ppdklssvmi
      181 qpgmtytiav sqekverlpa pyksncidyi srgnqkkyfg yytqdicvqn cemslemkhc
      241 qcvrthhefa atfggqvcdi irddictrkv eslgtyqfcl sqcrvacqet kydvrlssla
      301 vdksdfgkdm nkfsfiikfs sdtqrvlqyq pnlsiveafg yfggyvgiwl gfsllsvlln
      361 leahmakgfa krlkrvktad eavvvfrlkq rf
//
LOCUS       XP_077511306             541 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121780 [Amblyomma americanum].
ACCESSION   XP_077511306
VERSION     XP_077511306.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655180.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 27% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..541
                     /product="uncharacterized protein LOC144121780"
                     /calculated_mol_wt=59834
     CDS             1..541
                     /gene="LOC144121780"
                     /coded_by="XM_077655180.1:1..1626"
                     /db_xref="GeneID:144121780"
ORIGIN      
        1 mdgdvtkkrr kryfykdepf vvpkttlyrr qqevrlraqh casstsaasa andgdvnggg
       61 vvgdllnlss geqpaeeqaa qpasptlsah deqaggssya ddedlfrtdd fdrlgetped
      121 sssmsgeten ddgerdflas dfveleaail pgstttkaaa iimimafvvt hgltwealnd
      181 llrlidglfg fkgdvlprsk fvfrklwssr kerlvkrffy cdtcgsvlvs vpgmssmrcp
      241 dcevstelcv lkarghffii lgmkeqmkcl lakskaalfe rlvnlkaviq qggaalwqdi
      301 tsgsmaeelr krgkigwmdl titintdgsp vfkslsssvw piqflinelp pcdrlkncli
      361 gglwfgqhpy mrtfltkfve einqfgkitw kaghtllssg lhvlcccvda parasvinmv
      421 qfnglfgcpw cyncaefheg aqrymsvtvg eertpgemsk dmefaeikda vnglkgqspl
      481 nhlkhfnivf ghtveymhcv higvtkcltd qwfdsansqq pfyiaasaif saslcefvcs
      541 m
//
LOCUS       XP_077511313             417 aa            linear   INV 25-NOV-2025
DEFINITION  acid-sensing ion channel 1A-like [Amblyomma americanum].
ACCESSION   XP_077511313
VERSION     XP_077511313.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655187.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 56% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..417
                     /product="acid-sensing ion channel 1A-like"
                     /calculated_mol_wt=48017
     Region          10..383
                     /region_name="ASC"
                     /note="Amiloride-sensitive sodium channel; pfam00858"
                     /db_xref="CDD:459966"
     CDS             1..417
                     /gene="LOC144121787"
                     /coded_by="XM_077655187.1:1..1254"
                     /db_xref="GeneID:144121787"
ORIGIN      
        1 msasiclrvf iwtgclvgfl yqtssmlslf feypfsvtlv edvrknllfp aatvclhrwi
       61 disklcrhyv sqcageeetl ldperwhflr kevearlkva hlpdelflch mrskvtecas
      121 facnaairlt yyrhpnemcy tidayqwtns qhpvqrcesp wnyelvlraf wernatvalh
      181 epdalplvvh spyscppdkl ssvmiqpgmt ytiavsqekv erlpapyksn cidyisrgnq
      241 kkyfgyytqd icvqncemsl emkhcqcvrt hhefaatfgg qvcdiirddi ctrkveslgt
      301 yqfclsqcrv acqetkydvr lsslavdksd fgkdmnkfsf iikfssdtqr vlqyqpnlsi
      361 veafgyfggy vgiwlgfsll svllnleahm akgfakrlkr vktadeavvv frlkqrf
//
LOCUS       XP_077511369             327 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121828 [Amblyomma americanum].
ACCESSION   XP_077511369
VERSION     XP_077511369.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655243.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 37% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..327
                     /product="uncharacterized protein LOC144121828"
                     /calculated_mol_wt=34941
     CDS             1..327
                     /gene="LOC144121828"
                     /coded_by="XM_077655243.1:1..984"
                     /db_xref="GeneID:144121828"
ORIGIN      
        1 mhrraaslae lpldceslat eaactgerqa sqsghwiasr wpprlhaper gkpgraatgl
       61 rvlgnrgcmh rraaslaerp ldcesfatea actgerqasq sghwiaspwp prlhapesgn
      121 pgraatglrv lgnrgcmhrr aaslaekppd cefqateaac tgerqawqsr hwtaspwppr
      181 lhvperskpg ravsrepsah rpprcfdgeg fsaegpdsal qrtgscrsls avpptstctm
      241 visrkapsaa pvtssttaat glavrqkrgr lgrsgrgskh gcfgcaaasa ftraiashqd
      301 alhpfhdnli paflkmqgvw twlvlts
//
LOCUS       XP_077511378             201 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC144121835
            [Amblyomma americanum].
ACCESSION   XP_077511378
VERSION     XP_077511378.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655252.1
KEYWORDS    RefSeq; corrected model; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio            :: 17% of CDS bases
            frameshifts          :: corrected 1 indel
            internal stop codons :: corrected 1 genomic stop codon
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..201
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..201
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC144121835"
                     /calculated_mol_wt=22189
     CDS             1..201
                     /gene="LOC144121835"
                     /coded_by="XM_077655252.1:1..606"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon;
                     substituted 1 base at 1 genomic stop codon"
                     /db_xref="GeneID:144121835"
ORIGIN      
        1 mhrvirascm pllpqdniki vlrpkdglnl qnigptltte diicpnitqn iivastpsre
       61 navkyaavra iriadkdyav sayetaprdt ckgviryidt gddhrtiern tmndrnrlal
      121 aakriknsrx liilfdglrv pnfvrygptl vpcylyhkqv dicfvcgkfg hradvcpepg
      181 stecrgcgip nptpshvcds k
//
LOCUS       XP_077511388             199 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121842 [Amblyomma americanum].
ACCESSION   XP_077511388
VERSION     XP_077511388.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655262.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 49% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..199
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..199
                     /product="uncharacterized protein LOC144121842"
                     /calculated_mol_wt=21896
     CDS             1..199
                     /gene="LOC144121842"
                     /coded_by="XM_077655262.1:130..729"
                     /db_xref="GeneID:144121842"
ORIGIN      
        1 mqesvemped pahtgqeivf eyednlipvp atipctleeg ndstagsepa greqgtsger
       61 cgksnirrkr psssllrelm amhqqaeera akaakessel rkqlielqkg snevqkdglw
      121 pspvfymhhi qpvrvtavav dsfyalreac dccklpemfg qlgrqrsseq nvkaqtdgdl
      181 lgsvipvepl iavtklfml
//
LOCUS       XP_077511453             134 aa            linear   INV 25-NOV-2025
DEFINITION  piwi-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077511453
VERSION     XP_077511453.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655327.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..134
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..134
                     /product="piwi-like protein 1"
                     /calculated_mol_wt=15012
     Region          <31..117
                     /region_name="Piwi-like"
                     /note="PIWI domain. Domain found in proteins involved in
                     RNA silencing. RNA silencing refers to a group of related
                     gene-silencing mechanisms mediated by short RNA molecules,
                     including siRNAs, miRNAs, and heterochromatin-related
                     guide RNAs. The central component...; cl00628"
                     /db_xref="CDD:412485"
     CDS             1..134
                     /gene="LOC144121893"
                     /coded_by="XM_077655327.1:1..405"
                     /db_xref="GeneID:144121893"
ORIGIN      
        1 mewvinpiva slrglcpgvk hklalvvhvt npppgtvvds evtrperydf flvsqsvrqg
       61 tvaptqynvi ydttglkpdh mqrlpyklth lyfnwpgtir vpapcqyaqk laflagqslh
      121 aehnlrlsst lfyl
//
LOCUS       XP_077511467             173 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121898 [Amblyomma americanum].
ACCESSION   XP_077511467
VERSION     XP_077511467.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655341.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..173
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..173
                     /product="uncharacterized protein LOC144121898"
                     /calculated_mol_wt=17540
     CDS             1..173
                     /gene="LOC144121898"
                     /coded_by="XM_077655341.1:138..659"
                     /db_xref="GeneID:144121898"
ORIGIN      
        1 mgavmgvavq rlfslrgrkg krpfvgtklc rvatdaicer qgvdilaaqg figrwlpgac
       61 drgggrkrry aqafeppesh aqappvnpca gsapcpgaps ascpatppgm ailspsgvhp
      121 ssapptasss rpatvhpssa pspappptas ssrpatprst latwviapra hek
//
LOCUS       XP_077511488             473 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121920 [Amblyomma americanum].
ACCESSION   XP_077511488
VERSION     XP_077511488.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655362.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..473
                     /product="uncharacterized protein LOC144121920"
                     /calculated_mol_wt=53372
     Region          271..351
                     /region_name="retropepsin_like_LTR_1"
                     /note="Retropepsins_like_LTR; pepsin-like aspartate
                     protease from retrotransposons with long terminal repeats;
                     cd05481"
                     /db_xref="CDD:133148"
     Site            278..280
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133148"
     Site            278
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133148"
     Region          429..>465
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     CDS             1..473
                     /gene="LOC144121920"
                     /coded_by="XM_077655362.1:1..1422"
                     /db_xref="GeneID:144121920"
ORIGIN      
        1 meqlkppdql lltgniankw alfkekfelf leatastkep rteaaktall lsvagddale
       61 vfnnftfsae eskndyatvv skfeaycqeq qnevyeryvf rsriqaegep feqflrdlrk
      121 laqscnfstl sdgmirdqiv fgtnnpklrk klfkvenltl lkaeqsckaa evsahqnetw
      181 gqmerqvasv krrerfrcsr cnrlhernkc pafgktcfac kganhfaacc qkepavsavv
      241 hdeenfdiln vrvctvdrta dwtveakvsn qnvvfkvdtg sqanllpysv frkfkatpcl
      301 tpstsvlrsy sgdvikhfgv atlpvvindk sgtftffivk kskqailglh atkalgfvvr
      361 svnavdtaks dkllqefspl fqgtgcvarp yrmvlredai pviqparrip lalreplree
      421 lqrmeeahii ervneptewv splvivrkkd gklrlcmdpr nkrmpetgal sda
//
LOCUS       XP_077511498             220 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121927 [Amblyomma americanum].
ACCESSION   XP_077511498
VERSION     XP_077511498.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655372.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..220
                     /product="uncharacterized protein LOC144121927"
                     /calculated_mol_wt=23785
     CDS             1..220
                     /gene="LOC144121927"
                     /coded_by="XM_077655372.1:1..663"
                     /db_xref="GeneID:144121927"
ORIGIN      
        1 midsgssgcl lrasaagrcg arltkdttal ygfgsqgtps sraigrcytn lkidgvkgei
       61 lvdmregein vpiftsggkd vlvkrgrrpg rvievdvvei pdgqnqpacm svsdttepkv
      121 fcvatqrrpi edqqlvvgls vtneqksell gllneyrdcf annisklgrt tvlkmditet
      181 hgslpvavrp yrtnaieret ireiagewkr adivsetssp
//
LOCUS       XP_077511552             562 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121952 [Amblyomma americanum].
ACCESSION   XP_077511552
VERSION     XP_077511552.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655426.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 36% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..562
                     /product="uncharacterized protein LOC144121952"
                     /calculated_mol_wt=61626
     Region          <253..307
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     CDS             1..562
                     /gene="LOC144121952"
                     /coded_by="XM_077655426.1:268..1956"
                     /db_xref="GeneID:144121952"
ORIGIN      
        1 megsaaalth gsqvsqmcvq vegeeitpee frrspgwlhi gghtsrtrlq gtqgestsra
       61 dtpssrqkpk fnknvrsavi raarmpampv eeikivvrpr dgldifkaga acvanaimaa
      121 agietadrae dmacpntqqn imvlstpske raekyariqa invrgkiyev raygttahdt
      181 vkgvirgial edseaeimqq ivtkynplaa gakrignttt vivvfngikv pkyvrygstl
      241 ypcslyrkqi evckvcgkvg hrrdvcptpd vrvclacgvs npreghkcvp kcklcgeehp
      301 tgdrlcraky kvpyvvrrrr werrnaeeeq kqalesssfp rlgrsrsssa srsgsrsasr
      361 sasrhasrha srhasrsasr grdssdgnsf gvqrsrsrdr vswadaakgg vksssassss
      421 atnatlvett rrlaqelaea kkelqaqraq patvpaqsse trtpaviteq ktdtpshqgt
      481 qsmdttqdep qpeqttdptp kkrartssrq ykyqletqle akieafanty astaavtdqr
      541 larleelitt sfctiqerma rn
//
LOCUS       XP_077511561             758 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077511561
VERSION     XP_077511561.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655435.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..758
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=85984
     Region          41..198
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          291..673
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..758
                     /gene="LOC144121967"
                     /coded_by="XM_077655435.1:1..2277"
                     /db_xref="GeneID:144121967"
ORIGIN      
        1 mrptkkarff dnirstikql tstggggftr qlleadisad cvlgllqfmr afrelepwal
       61 rqpdkkkntr lkqltvhgtr nkfslsrhni havfdataky ptgflqatsa elgaydecie
      121 tvvrdedgae kvrgqycnmr lsitdedsfl eeilpaiaft hrraskfgsy fvdkklpglr
      181 lglcfvsscs aqdltniaqt magnrakivv dncvtsvddg isgtqagiia ylailamviv
      241 aatgldllte rwdknrknai pykcamafsa lsnsrlimgi nkdknsdnys frfmhgirfl
      301 sifwiclghs ygtisenfsr mlnavqyfer wetlivtagy lavdtfffls gfllyytlsr
      361 qqrnravvtv vaiirryvri tvplffmlmc myllpliasg pnskdlynrf yaeirkhwwd
      421 llflmrnwra dqevtilphl wylsidyqlf lvgvvviqvl ktrkwlvact fallslvccg
      481 isaweiygtn mtpfvvavae nfstvvdtln ysylhpfyhg vcffsgcitf llvekyskak
      541 iskmivaslw cislfcglyc lfmklewyrs nergsetrrt fhaftdrifw svgvawfvft
      601 catgrgglvn rflswdgfvp lsrlsfcvyl ihspifvvty hvareriffs hytlvsqcfa
      661 vlvwsyivsy flfiacdapt ghleklvfmp qgrretsqng apangvsnrg dsngnnvkdl
      721 pigiieegvi knnfngvsls pgdaaecdgs ctkkcyrl
//
LOCUS       XP_077511572             105 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121976 [Amblyomma americanum].
ACCESSION   XP_077511572
VERSION     XP_077511572.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655446.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 34% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..105
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..105
                     /product="uncharacterized protein LOC144121976"
                     /calculated_mol_wt=10862
     CDS             1..105
                     /gene="LOC144121976"
                     /coded_by="XM_077655446.1:99..416"
                     /db_xref="GeneID:144121976"
ORIGIN      
        1 mvqgdarlde sdgestvifq sedglsdatl laspapdisa agpstederv appcsldevs
       61 epaarrrqgg ravaveqpls aelearlqaa eddkrsgslk eqatl
//
LOCUS       XP_077511598             138 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144121988 [Amblyomma americanum].
ACCESSION   XP_077511598
VERSION     XP_077511598.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655472.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..138
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..138
                     /product="uncharacterized protein LOC144121988"
                     /calculated_mol_wt=16071
     CDS             1..138
                     /gene="LOC144121988"
                     /coded_by="XM_077655472.1:1..417"
                     /db_xref="GeneID:144121988"
ORIGIN      
        1 mplvdtpfrr vaidiigplk pttrkghhfi ltivdyetrf peavalpsie terkwavgcl
       61 ftsrttspyh pmanglverl nwtlkkmlrr mcteqpkewd rfiepllfay revpqeydfg
      121 vesikeknnv dsdylsrv
//
LOCUS       XP_077511610             238 aa            linear   INV 25-NOV-2025
DEFINITION  PR domain zinc finger protein 14-like [Amblyomma americanum].
ACCESSION   XP_077511610
VERSION     XP_077511610.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655484.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..238
                     /product="PR domain zinc finger protein 14-like"
                     /calculated_mol_wt=26583
     Region          18..120
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(71..76,87..89,110..113)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Site            order(161,163,165..166,169..170,173,187,189,193..194,
                     197..198,202,216,218,220,222..223,226..227)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          166..188
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          182..203
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          211..228
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..238
                     /gene="LOC144121993"
                     /coded_by="XM_077655484.1:1..717"
                     /db_xref="GeneID:144121993"
ORIGIN      
        1 mfeflasgks tvkgvpvgvf tlkalpkglh igpyhgvkvd cienggctwp vrrcgdlfvv
       61 egrpqqrnhw mndvnyspsm rrqnlvallf ngdiyyrtlr nvgpdeellv gystsfaksl
      121 lgnprgpgtl kraagylvpp gtvrlmceca vaaralgtmr fsrpdnlkvh mmiysgekpy
      181 ecsqcgkrlr ervharrhee vihsrryqln cprcgagved vtrlrrhrck plgategk
//
LOCUS       XP_077511627             212 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122002 [Amblyomma americanum].
ACCESSION   XP_077511627
VERSION     XP_077511627.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655501.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 15% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..212
                     /product="uncharacterized protein LOC144122002"
                     /calculated_mol_wt=23785
     CDS             1..212
                     /gene="LOC144122002"
                     /coded_by="XM_077655501.1:1..639"
                     /db_xref="GeneID:144122002"
ORIGIN      
        1 mapprdpgtf sgtdgsdved winlyervsn ynrweptlll anvifylngt arvwyethee
       61 elrswdtfke klktlfgrpd drqlaakkql atraqsstgs yvayiqdila lchkvdaats
      121 etdkvghvlk giaddafhll vckdctsids iinecrrfeq vkgrrispsf srlpntatts
      181 scedhrspsr tpsdapavtd sltrivrrcs ql
//
LOCUS       XP_077511640             192 aa            linear   INV 25-NOV-2025
DEFINITION  carnitine O-acetyltransferase-like [Amblyomma americanum].
ACCESSION   XP_077511640
VERSION     XP_077511640.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655514.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..192
                     /product="carnitine O-acetyltransferase-like"
                     /calculated_mol_wt=21222
     Region          <1..175
                     /region_name="Carn_acyltransf"
                     /note="Choline/Carnitine o-acyltransferase; pfam00755"
                     /db_xref="CDD:459926"
     CDS             1..192
                     /gene="LOC144122008"
                     /coded_by="XM_077655514.1:126..704"
                     /db_xref="GeneID:144122008"
ORIGIN      
        1 mamqlayyki hrapgatyes astrkfvhgr tetirstsse slkfckafes getklpekve
       61 rlrkavqnhk dytnlaingc gvdrllfglk miaieneipv peiykdaayk istefkisss
      121 qvvtqcdavm fsgplvkdgy gccynpqahi vtfglssfns saatftakfr daleesltqi
      181 hdclrlsqas kl
//
LOCUS       XP_077511654             509 aa            linear   INV 25-NOV-2025
DEFINITION  carboxy-terminal kinesin 2-like [Amblyomma americanum].
ACCESSION   XP_077511654
VERSION     XP_077511654.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655528.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 11% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..509
                     /product="carboxy-terminal kinesin 2-like"
                     /calculated_mol_wt=56797
     Region          <25..>167
                     /region_name="COG4913"
                     /note="Uncharacterized conserved protein, contains a
                     C-terminal ATPase domain [Function unknown]"
                     /db_xref="CDD:443941"
     Region          164..502
                     /region_name="Motor_domain"
                     /note="Myosin and Kinesin motor domain; cl22853"
                     /db_xref="CDD:473979"
     Site            order(174..175,251..258,363..364,406..411)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:276817"
     CDS             1..509
                     /gene="LOC144122014"
                     /coded_by="XM_077655528.1:1..1530"
                     /db_xref="GeneID:144122014"
ORIGIN      
        1 mkmrvvsehh trvartysgv lknhqekdrq aedpefptia etqqqveegr tlflqgttea
       61 kltsevserd slalqltsav ghaatlqrsl aeatgalagl rlectalmap qeyrevqrat
      121 laadkgrles lvadlcakaa rkqeqlnasa qerrathnsl qklkgnirvf chlrfllppe
      181 kapqrpfltl pderttemlr tnpeaqqerf ttvsfdrvfp gvasqaavya evsqlvqsal
      241 ngyhvcifay gqagagktnt megdeddelr giilralhqv feasqkqpqw tytmvvsfve
      301 vynetqrdll vppsqadkap plrlmmiskg epvtvaslte vpvksaqqig qllwcarknr
      361 avaatkcner ssrshpvfrp ditghnehte lscrvwlwtr rlnrvnlass erlsesqsvg
      421 arlreahnin rslanlgnvi ralynradhv pyrdsklthl lidslrgnsk tlmllnispr
      481 vdtigetins lrfattanrc higtarknl
//
LOCUS       XP_077511727             335 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122081 [Amblyomma americanum].
ACCESSION   XP_077511727
VERSION     XP_077511727.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655601.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 14% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..335
                     /product="uncharacterized protein LOC144122081"
                     /calculated_mol_wt=37364
     Region          <6..61
                     /region_name="SCAN"
                     /note="SCAN domain; pfam02023"
                     /db_xref="CDD:460417"
     CDS             1..335
                     /gene="LOC144122081"
                     /coded_by="XM_077655601.1:1..1008"
                     /db_xref="GeneID:144122081"
ORIGIN      
        1 msnmqewlke ekafgdheki lqcfgleqfy srlpenvryw vldrpdvstv akaaelaeef
       61 vtrgargakd gqkgefgsks erpkftpmra rgdtrsadas esspterket aaaeaerrkr
      121 fetrqarvcy tfqkpghfsa qcpetkskvv flslcstden mkllepymrd flvngkecrv
      181 lrdsaatmdv vhpsyvepdm ftgecawikq aveahsvclp vakvliegpf gaieteaavs
      241 smlppqypyl fsnrsdhllr ekgllfgeas vqaltrsrvr elaakavvag ptlsnnekgs
      301 eaqqadtqsi kedivssfqq psvlemvkgk ftnqq
//
LOCUS       XP_077511737             309 aa            linear   INV 25-NOV-2025
DEFINITION  carboxy-terminal kinesin 2-like [Amblyomma americanum].
ACCESSION   XP_077511737
VERSION     XP_077511737.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655611.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..309
                     /product="carboxy-terminal kinesin 2-like"
                     /calculated_mol_wt=33468
     Region          59..299
                     /region_name="Motor_domain"
                     /note="Myosin and Kinesin motor domain; cl22853"
                     /db_xref="CDD:473979"
     CDS             1..309
                     /gene="LOC144122088"
                     /coded_by="XM_077655611.1:1..930"
                     /db_xref="GeneID:144122088"
ORIGIN      
        1 meakltsavs erdslalqlt savgqaatlq rslaeatgal aglrlgctal maqreriptf
       61 sfdrvfpgva sqaavyaevs qlvqsaldgy hvftfaygqa gagkthtmeg deddelrgii
      121 pralrqvfea sqkqpqwtyt mvvifvevyn etqrdllvpp sqadkapplq ltmtskggpv
      181 avasltgasq vsptvwlwtr rlnrvdlags erlsqsqsag arlweahnin rslanlgnvi
      241 ralcnqadhv phrdsklthl rmdslggnsk tlmllnispq vetigetins lrfattanry
      301 hiatarknl
//
LOCUS       XP_077511775             200 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122118 [Amblyomma americanum].
ACCESSION   XP_077511775
VERSION     XP_077511775.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655649.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 32% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..200
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..200
                     /product="uncharacterized protein LOC144122118"
                     /calculated_mol_wt=23197
     CDS             1..200
                     /gene="LOC144122118"
                     /coded_by="XM_077655649.1:266..868"
                     /db_xref="GeneID:144122118"
ORIGIN      
        1 mykeqtedyk rreaerlmqi vqeslegdrr qdaaeeereh ppprvskddt shaaarglgh
       61 qerlqgdgvg cergyaslsl stlslgeaac rtgteadesv eeenweldit pwtsryiprg
      121 hgyfqwarhc srrtrrrmyk eqtvdykrre aerlmqivqe slegdrrqda aeeerehppp
      181 riqgqdrara prvsgvedvf
//
LOCUS       XP_077511800             257 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122128 [Amblyomma americanum].
ACCESSION   XP_077511800
VERSION     XP_077511800.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655674.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 39% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..257
                     /product="uncharacterized protein LOC144122128"
                     /calculated_mol_wt=28398
     Region          20..>125
                     /region_name="GAS"
                     /note="Growth-arrest specific micro-tubule binding;
                     pfam13851"
                     /db_xref="CDD:464001"
     CDS             1..257
                     /gene="LOC144122128"
                     /coded_by="XM_077655674.1:1..774"
                     /db_xref="GeneID:144122128"
ORIGIN      
        1 mllgqefmsn fsemllefvr nqkeckegwh aaevenselk tlittltkkn lelkqqsana
       61 realkreqlr rekcekevev qkkqlevvre flekktkmln nilqklplqe pgqeaklhrr
      121 gnrlevtrnc ggsmvpssgg cfpknksghc ganstslpek nnvhgtvfkr plpvakkekg
      181 idkeqahikg pvsklqkaea ikngkkvlap sravnqpvtp cqppplptas vskppccpta
      241 gapprclptk pkftsgq
//
LOCUS       XP_077511813             142 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122133 [Amblyomma americanum].
ACCESSION   XP_077511813
VERSION     XP_077511813.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655687.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..142
                     /product="uncharacterized protein LOC144122133"
                     /calculated_mol_wt=15591
     CDS             1..142
                     /gene="LOC144122133"
                     /coded_by="XM_077655687.1:2558..2986"
                     /db_xref="GeneID:144122133"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrphfa altsvhccqr payigpcvlp afgcvippdp
       61 fmlktkscme vvqatklrvi fardlkqark imdgqwysls kanffsktkt iflgswamyl
      121 gtcgqdntgq hasasskkst kq
//
LOCUS       XP_077511837             598 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122143 [Amblyomma americanum].
ACCESSION   XP_077511837
VERSION     XP_077511837.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655711.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..598
                     /product="uncharacterized protein LOC144122143"
                     /calculated_mol_wt=64799
     Region          <37..156
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            41
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238827"
     Region          320..447
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     Site            order(323..326,356..358,361,386,425,443)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260008"
     Region          <512..544
                     /region_name="zf-RVT"
                     /note="zinc-binding in reverse transcriptase; pfam13966"
                     /db_xref="CDD:433612"
     CDS             1..598
                     /gene="LOC144122143"
                     /coded_by="XM_077655711.1:1..1797"
                     /db_xref="GeneID:144122143"
ORIGIN      
        1 mstsnnllqh vrgvladrrg cvrlgnvtss lrhtwrgvpq esvlspllfn avlallpnfl
       61 pkdlrlpihv aiyandiaiw crgprryrvh tiknvqrain avaaflerag lsispsktqa
      121 mllntrvgar pgrtflqvgg rnlawsrqvk ylgllidvrl twnpavshfl aqssriqgti
      181 rsllakgngi tprlglqlyq gmavpqfaya lplvrltvaq hlaierklra airlclglpr
      241 sspvaatlae avgqaaqyfe divggepesf pipprpdlgh lyevhlslpg srtrrntaav
      301 glqqevaevl qaeppgtvpi ftdgsvlpgp cpsaaaacta pslhssrqar lpfvasstta
      361 elaaihlaad ilnehpgikd aaiytdsraa lqlllrpfkg ppiarrlawr ldklctrgtr
      421 irliwvpthv gvlgneeads lakaahspgt plsknlcsld larsivdrll lldhpdprva
      481 nghppyplpn lgllckersl llrlrvgcan igsrlfrigl sdspgcslcn ypeettqhll
      541 cdcpafvser timvahyrsi glpstsaddl lfpgghhpvk afrhllsfle atglsgrl
//
LOCUS       XP_077511866             270 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122162 [Amblyomma americanum].
ACCESSION   XP_077511866
VERSION     XP_077511866.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655740.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 27% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..270
                     /product="uncharacterized protein LOC144122162"
                     /calculated_mol_wt=28405
     Region          20..104
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..270
                     /gene="LOC144122162"
                     /coded_by="XM_077655740.1:1..813"
                     /db_xref="GeneID:144122162"
ORIGIN      
        1 maasgsaect rsksrssiyc cvygchnsyk ntagklpkik fssfpwrpye eerrqrwvra
       61 vrraspdgsp wqpnrcstri csahfvgnek stiaghpayi ptlfpasygr cdgvcsetki
      121 gryeraqrrg ltteastsgk lrtaaepvgs qsavsgadgl saeatapdep ettadasspk
      181 aaasgaggar sssdsvatml svatqtegsl hqgqctlfls vasqgcpstq vchndtvdts
      241 vqaqvatest fagpeerscv flgygytlkg
//
LOCUS       XP_077511876             186 aa            linear   INV 25-NOV-2025
DEFINITION  myb/SANT-like DNA-binding domain-containing protein 3 [Amblyomma
            americanum].
ACCESSION   XP_077511876
VERSION     XP_077511876.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655750.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..186
                     /product="myb/SANT-like DNA-binding domain-containing
                     protein 3"
                     /calculated_mol_wt=20518
     Region          31..108
                     /region_name="GT1"
                     /note="myb-like, SANT family; cl23759"
                     /db_xref="CDD:474047"
     CDS             1..186
                     /gene="LOC144122170"
                     /coded_by="XM_077655750.1:1..561"
                     /db_xref="GeneID:144122170"
ORIGIN      
        1 mtqpsqqpls qqsqlplssl dnnaspqrka aprftveekr lltsvvnehk rfleckktdv
       61 aslakksevw kevakifnsh hgvtrrdhlq lkkcwanmkq kwkeedakqk relhktgggp
      121 apaamdplte qvgavaghma triynefdsd gandlppvht lpvvrllqpm idgggdtecd
      181 ftggge
//
LOCUS       XP_077511885             251 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122179 [Amblyomma americanum].
ACCESSION   XP_077511885
VERSION     XP_077511885.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655759.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..251
                     /product="uncharacterized protein LOC144122179"
                     /calculated_mol_wt=28121
     Region          52..131
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     Region          202..236
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..251
                     /gene="LOC144122179"
                     /coded_by="XM_077655759.1:1..756"
                     /db_xref="GeneID:144122179"
ORIGIN      
        1 mvalqahvrp hhkrrgrrwh aapkpdpddd dqysvpsgpr ngllqrkarp gvkkhtqlwd
       61 qhsklhkeag yreaawmeia aalavtveec qtrwrtlrdt ylkrkkrrri dpkgqwsvld
      121 relgflddfl rprtrrstln aaapadvevk qeqpgedaem reagvaaaaa ppqscplamr
      181 paavmphrla aggaaaqals sdpeelfcls laarlkrllp rerhmarmkm lqtlhdlesg
      241 enigefalgd p
//
LOCUS       XP_077511892             122 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122188 [Amblyomma americanum].
ACCESSION   XP_077511892
VERSION     XP_077511892.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655766.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..122
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..122
                     /product="uncharacterized protein LOC144122188"
                     /calculated_mol_wt=14452
     CDS             1..122
                     /gene="LOC144122188"
                     /coded_by="XM_077655766.1:29..397"
                     /db_xref="GeneID:144122188"
ORIGIN      
        1 magadddvlt tmsvlvivcs lllrrrraqk arrrrfwvhp cwryrdvegq anallprlra
       61 rdegffrdfl rmppssfdtl lhlvrpvier qdtpfrrsis ahdrlamtvr tingwndrlh
      121 wr
//
LOCUS       XP_077511901             263 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122192 [Amblyomma americanum].
ACCESSION   XP_077511901
VERSION     XP_077511901.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655775.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..263
                     /product="uncharacterized protein LOC144122192"
                     /calculated_mol_wt=28311
     CDS             1..263
                     /gene="LOC144122192"
                     /coded_by="XM_077655775.1:596..1387"
                     /db_xref="GeneID:144122192"
ORIGIN      
        1 mrkiffaate asakfknard rwlkivsgtd atcrsgapgn ggnatkkwal fplmdgmlrs
       61 tphyasritt nvpkktwqsp gpsqrsgsgf atpestytsq sspgmellei adintaspae
      121 tqllglyddt vvnpdeddcs papaqarcag pgspspdeme vlgryaapvr lskgtysggp
      181 psrrrkktdd sfedcvkatl dkcagslani tsnqrtevqc edesdatgrl vtvklralpp
      241 rqrsqaifqi skilyeaeqe tle
//
LOCUS       XP_077511909             192 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122197 [Amblyomma americanum].
ACCESSION   XP_077511909
VERSION     XP_077511909.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655783.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..192
                     /product="uncharacterized protein LOC144122197"
                     /calculated_mol_wt=21883
     CDS             1..192
                     /gene="LOC144122197"
                     /coded_by="XM_077655783.1:38..616"
                     /db_xref="GeneID:144122197"
ORIGIN      
        1 meleglkqgl aflesesikv avlvtdrhtq ikcflrnnka aiahefdvwh makgitkklh
       61 taakqnncke lapwiksvcn hlywaaasse gkaelivpkw lsllnhirdi hthdqasfpt
      121 clhgdiepkq wlreeskafq klqdianska llqdmprlst rhqtygleaf hsllvhfapk
      181 tynysylgmr ar
//
LOCUS       XP_077511926             436 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122256 [Amblyomma americanum].
ACCESSION   XP_077511926
VERSION     XP_077511926.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655800.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..436
                     /product="uncharacterized protein LOC144122256"
                     /calculated_mol_wt=49121
     CDS             1..436
                     /gene="LOC144122256"
                     /coded_by="XM_077655800.1:75..1385"
                     /db_xref="GeneID:144122256"
ORIGIN      
        1 mvsyfsiilv fpqmfeedfn vfvdipedfe irdrakikvs edcqvriadv ldepqqaeqs
       61 rvtatyslpa vpndikfsie rhqsgqyfra rsrvvqwlyh dlclytmypg klyneaaqal
      121 vsrypnlads sgtgydswre alrfkakyer rkirieqndg adsppskktm kdgkvatvgg
      181 nalqrvsrps vatapdgede dsiaghiegm ksavqkarpd mayiidcmrr tfptrrkwig
      241 sedpsvevai ekfpalamss iaqlefelit svpvlerlee alqrtkekiv haarkkrhle
      301 gfledfdarm dgtseaavye ttvtaavcll psmvkervet fvrpfdpaav hyiptvvhrg
      361 giltttdfsv clekiciket sllaavatqm alywafnivf dkkaqrsfdl lcrlinvdsg
      421 lrptplvrla qtvlgk
//
LOCUS       XP_077511928             272 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122337 [Amblyomma americanum].
ACCESSION   XP_077511928
VERSION     XP_077511928.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655802.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 21% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..272
                     /product="uncharacterized protein LOC144122337"
                     /calculated_mol_wt=30139
     CDS             1..272
                     /gene="LOC144122337"
                     /coded_by="XM_077655802.1:1..819"
                     /db_xref="GeneID:144122337"
ORIGIN      
        1 mphrpvtwlp phqreesgrp ksgcgvtarc lkagtssvsm pkttnwppcg svrdrqrqne
       61 lfsrekgisf ddecreilse ewvrrresll irrslledel qlkdletkqm nrithaghei
      121 gpmwcsseps elqveeeava nvipvtqwld eerppddfep etevqvsagw hpvidfsfqa
      181 qqnedfpgts aanachsrha shkcslsgss ldegqpkllt srssvldsnl cfldppspsy
      241 lalpsiaars apksddedtd agvsavekpa qn
//
LOCUS       XP_077511929             348 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122353 [Amblyomma americanum].
ACCESSION   XP_077511929
VERSION     XP_077511929.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655803.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 35% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..348
                     /product="uncharacterized protein LOC144122353"
                     /calculated_mol_wt=38945
     Region          105..197
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     CDS             1..348
                     /gene="LOC144122353"
                     /coded_by="XM_077655803.1:1..1047"
                     /db_xref="GeneID:144122353"
ORIGIN      
        1 mppgmestaq paqpcsshaa pqselpgqan pqeavlrnal rliesltgiv qnnlqtpaav
       61 arprlhvdlp syggyhdlvs aneyldrmln yqqatgltdg evlarivpvs ltdqaarwfg
      121 ltghrsrtme efrarfreef lpadydrrlr relelrtqhp deslleyvra mdelyriadp
      181 sapngekver vtrqarptfa aylggvqfrd ldelasaakr iqanilasra yrppppasqa
      241 leprcawngy tfrirsrgae tvafsdgqlr ndwelsdral dpytyamraa haarsqerqr
      301 nhsflapele nnsledsvel lvrsmaakek qggtaepqqp psnvtdpw
//
LOCUS       XP_077511930             288 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122431 [Amblyomma americanum].
ACCESSION   XP_077511930
VERSION     XP_077511930.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655804.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..288
                     /product="uncharacterized protein LOC144122431"
                     /calculated_mol_wt=32605
     CDS             1..288
                     /gene="LOC144122431"
                     /coded_by="XM_077655804.1:1..867"
                     /db_xref="GeneID:144122431"
ORIGIN      
        1 mgpgkrekwr crvcrgeslp vkpndkptee psdvfkkvaa leeklmvvse lkisidslla
       61 lraqldklvm tiqglqmtvt evqtsveffa skydtllatc ndavqatktl eqkvaflegt
      121 ideqasmidr iqwelneseq ysrlsnleis glpvqpqedl raevaqmaet levrdfnqse
      181 iaslhrlssr qgkipvvlvr fsspslrekw lqvksklrtl neshklssvf vnenltrmns
      241 elfwkarirg keksfkfvwv kngkvfarke vgasvikias lsdidkmt
//
LOCUS       XP_077511931             451 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122481 [Amblyomma americanum].
ACCESSION   XP_077511931
VERSION     XP_077511931.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655805.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..451
                     /product="uncharacterized protein LOC144122481"
                     /calculated_mol_wt=50208
     Region          58..>436
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..451
                     /gene="LOC144122481"
                     /coded_by="XM_077655805.1:1..1356"
                     /db_xref="GeneID:144122481"
ORIGIN      
        1 mitveavlvp hcpgkpvlpv sghrqridvf tgsvigqelp shvfkvrkvi ltptrriymp
       61 pqfvcksrll qhfdpdyapr iivrdddckl vffslvackd dflsavikpp lssglniags
      121 rfmllgcsts qlcnhgvwfy ahdnqgrtae qirdgigdls tilsvakfma rlgqafsqpl
      181 glltvpsqnt svepdirhaa pgcrsdsshr eyifsdsigr ispgllrkhr pqqaprdths
      241 srgslqgssp tslrlrsdgv lylnrpmiti leqsgikaev flmlqnkiln sfidkmmdph
      301 eaahvlcvyc alrlpykera cgcidltiep ffralvravn kkvlkelrtk arilvlsdyg
      361 ltmfgvldet gtleygqvfv qysndrhatv qgdvivtksp cmhtgdipql kavnvpqlhh
      421 vrdctvfpqk gerplprrdd ryapflasal c
//
LOCUS       XP_077511933             204 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122576 [Amblyomma americanum].
ACCESSION   XP_077511933
VERSION     XP_077511933.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655807.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..204
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..204
                     /product="uncharacterized protein LOC144122576"
                     /calculated_mol_wt=22953
     Region          <74..198
                     /region_name="P2RX7_C"
                     /note="P2X purinoreceptor 7 intracellular domain;
                     pfam20478"
                     /db_xref="CDD:466627"
     CDS             1..204
                     /gene="LOC144122576"
                     /coded_by="XM_077655807.1:1..615"
                     /db_xref="GeneID:144122576"
ORIGIN      
        1 mvylpelqrr wleksrllgf epygdkplrv qdrpssedds cgsdngcede acgrqlavva
       61 rgttatcgsp dreppepgrv gnthwcvcgw cvsmptales tccreipaat vkqpsgcite
      121 hphfytlcld evvltvalqm llghglhveh trryryisys qlshwlwgrl drhhrkvlpa
      181 cavhqiregf psegytrfqy aqgl
//
LOCUS       XP_077511934             178 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122607 [Amblyomma americanum].
ACCESSION   XP_077511934
VERSION     XP_077511934.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655808.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..178
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..178
                     /product="uncharacterized protein LOC144122607"
                     /calculated_mol_wt=18685
     CDS             1..178
                     /gene="LOC144122607"
                     /coded_by="XM_077655808.1:1..537"
                     /db_xref="GeneID:144122607"
ORIGIN      
        1 mvmvlgiril schprreeel rpgglepqap dvsrrdpdgi vsavgpfvdq qqlhgvlaaa
       61 vrggtqrlal rsdhmvniqm edqgvrglcg rntalhighg nklssggsas rkdpfrqpgg
      121 tpwrawapge aaertprgsn rgqrvqhagg gcvkdkelhf lgcslllgsd ggapgrgg
//
LOCUS       XP_077511935             868 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122623 [Amblyomma americanum].
ACCESSION   XP_077511935
VERSION     XP_077511935.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655809.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..868
                     /product="uncharacterized protein LOC144122623"
                     /calculated_mol_wt=97159
     CDS             1..868
                     /gene="LOC144122623"
                     /coded_by="XM_077655809.1:1..2607"
                     /db_xref="GeneID:144122623"
ORIGIN      
        1 mvkptsngsv ssgqrsapst estawdntdd avgsteprdk vhstdypdvl piegaaqdga
       61 sgaaadaepp sydflneelq pesdsctdas snmsgdvgmn sdydfendel qpesdtcsra
      121 ssdvggdigl ssgslteese ssededdelp ssfaqfsnek lpnaevtkmg aiaavmvyav
      181 shgltwaalg dliklinflf gqnvlpqsty afrelwckek qdilqyhcfc etcgallnvi
      241 eksaqclncr ytsalqklkd rgsffiilnl hkqlsfaies tkvqlhenle klqepqsvis
      301 dvtsaqcysr mrerlpkddl tltintdrsp vwkssktsvw plqfivnelp phlrfkhpvl
      361 aglwfgkkhp nmqlflkkfv ievnttevvk wtyltttnys kpavlccsvd aparaavlnm
      421 vpfnryfgcp wcflrrehve gsmryiikeq pevrttymvi rdkklaegyd diingfkgps
      481 almnlgglhl vngfsvdymh svllgvvtqv petilstsns gqhfyvgrps tledidsrlf
      541 sikpphcvtr lprpirerrh wkasewrqwi lfyalpyleg ilhpdywrhl cklsealhil
      601 lrekltlsei akaelllesf viqckrlygv asltfsvhvl lhlancarsl gplwaysafv
      661 fedgnghivq qvsaansipq qiverliihq qlhkvmsssf lclhekdfcm dtfgyprlkr
      721 athvgklcll gkgndvalsa eeqdavnnrl gtstftalqy erfvqakqvf hstaykrpsr
      781 sdstfiktcs gsyeriekvl lleakvcvlm crpviiadrd aapphikecf vssgralnvv
      841 lpeevadccl fiefcskkrh sfatcrig
//
LOCUS       XP_077511936             338 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122639 [Amblyomma americanum].
ACCESSION   XP_077511936
VERSION     XP_077511936.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655810.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 23% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..338
                     /product="uncharacterized protein LOC144122639"
                     /calculated_mol_wt=36665
     Region          17..91
                     /region_name="Myb_DNA-bind_5"
                     /note="Myb/SANT-like DNA-binding domain; pfam13873"
                     /db_xref="CDD:433544"
     CDS             1..338
                     /gene="LOC144122639"
                     /coded_by="XM_077655810.1:5..1021"
                     /db_xref="GeneID:144122639"
ORIGIN      
        1 mslqssqdds csqrrlaprf tfeekelllg lvktyqhvle ckktdvssla kkkatwkeva
       61 glfnshhgvt rrdpnqlkkc wanmkqkske edatkkrdrh ktgggpakch vsaqseqvma
      121 vashistrag natdsdggid lppvaslpvi rllqpmvdep gdaechysed ewetpfmdqp
      181 qssvaalgrs gatpeaieqe snlffpavqp vaepadasst aaagcisatv asgaaagstt
      241 aeavatgepa raprgrmall ekspadendf rvallreere lrlklmreeh kstlderskr
      301 vmmstlsgat qdpplhfanl filplellpv ipitlkfl
//
LOCUS       XP_077511937             391 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122670 [Amblyomma americanum].
ACCESSION   XP_077511937
VERSION     XP_077511937.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655811.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 38% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..391
                     /product="uncharacterized protein LOC144122670"
                     /calculated_mol_wt=43122
     CDS             1..391
                     /gene="LOC144122670"
                     /coded_by="XM_077655811.1:145..1320"
                     /db_xref="GeneID:144122670"
ORIGIN      
        1 mihaggssya qrsvslssaa tstqhqsnhq krccnfgkrq gptydsqlfl mpcrvfeets
       61 srralfiqrl ssnvdgnrsk niqgvrrsll pseywallls lkpqpclvpr yhscsakavv
      121 wpfvaeqqqp vivaererki rdsplyaaaa gsskveghrv hgrrrssypr vekvqsgqlp
      181 hkipstadps trrpgeknlr shrnltrppe qpgcrpvlhl eskarnlppa rphlgevhad
      241 laapaalryp vypgecpalq fqeimnrrgf ktrhsnnagl iaptaagshr wyhpvwnwse
      301 tgdsafsedv errqgvgpdk vaaplfcrla ctgypperrr gggldklgap llcqpactsy
      361 plerrggggp dkmgapllcc sastgypheq r
//
LOCUS       XP_077511938             454 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122686 [Amblyomma americanum].
ACCESSION   XP_077511938
VERSION     XP_077511938.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655812.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 46% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..454
                     /product="uncharacterized protein LOC144122686"
                     /calculated_mol_wt=49510
     CDS             1..454
                     /gene="LOC144122686"
                     /coded_by="XM_077655812.1:77..1441"
                     /db_xref="GeneID:144122686"
ORIGIN      
        1 mihaggssya qrsvalssaa tstqhqsnhq krccnfgkrq gptydsplfl mpcrvfeets
       61 srralfiqrl ssnvdgnrsk niqgvrrsll pseywallls lkpqpclvpr yhscsakavv
      121 wpfvaeqqqp vivaererki rdsplyaaaa gsskveghrv hgrrrssypr vekvqsgqlp
      181 hkipptadps trrpgeknlr shrnltrppe qpgcrpvlhl eskarnlppa rphlgevhad
      241 laapaalrer rrggslgdlg apilcrpact sypqerrrrg gpyklsavll yclastgypk
      301 errrgggpdk mdapliccsa stgysqerrr gggadkmgap lfccpastgf pqerrrgdgp
      361 dkmgsplhcc sactgypqer rrgggadkmg alliccpast gypqerrrgg gadkmgprlf
      421 ifprllatqr sgdeeaapas wapgssvfpr plvt
//
LOCUS       XP_077511939             543 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122702 [Amblyomma americanum].
ACCESSION   XP_077511939
VERSION     XP_077511939.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655813.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 27% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..543
                     /product="uncharacterized protein LOC144122702"
                     /calculated_mol_wt=58936
     CDS             1..543
                     /gene="LOC144122702"
                     /coded_by="XM_077655813.1:77..1708"
                     /db_xref="GeneID:144122702"
ORIGIN      
        1 mihaggssya qrsvalssaa tstqhqsnhq krccnfgkrq gptydsplfl mpcrvfeets
       61 srralfiqrl ssnvdgnrsk niqgvrrsll pseywallls lkpqpclvpr yhscsakavv
      121 wpfvaeqqqp vivaererki rdsplyaaaa gsskveghrv hgrrrssypr vekvqsgqlp
      181 hkipptadps trrpgeknlr shrnltrppe qpgcrpvlhl eskarnlppa rphlgevhad
      241 laapaalrll agvvvvrllc tvwtvefwth isccflawvt ahsqaallvl lraagvtagt
      301 tlcgtgpkqe tppsrrtwsg dkesvptglv phssvvshal athrsgdegv asailaprss
      361 vgpraqatlr sgdvvvapts cllcffivsr platqrsgde eaaptkwmpr sfvastgysq
      421 errrgggadk mvhhsfvvpr plvfpqerrr gdgpdkmgsp lhccsactgy pqerrrggga
      481 dkmggpysfv vplplathrs gdeeaaptrw vpgclffrvs wlprgaergg cpaswapgss
      541 vfp
//
LOCUS       XP_077511940             372 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122718 [Amblyomma americanum].
ACCESSION   XP_077511940
VERSION     XP_077511940.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655814.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 34% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..372
                     /product="uncharacterized protein LOC144122718"
                     /calculated_mol_wt=41077
     CDS             1..372
                     /gene="LOC144122718"
                     /coded_by="XM_077655814.1:313..1431"
                     /db_xref="GeneID:144122718"
ORIGIN      
        1 mthaggssya qrsaalssaa tstqhqsnhq krccnfgkrq gptydsqlfl mpcrvfeets
       61 srralfiqrh srnvgrnrsk nvqgvrrtll ppedgawler ipalntqpci vpryhrgsak
      121 avvwpfvaeq hqavivaate rkirdsphya aapgsskveg hrvhgrrrss yprvekvqsg
      181 rlphtipstv dpstrrpgek nllshrnlrp ppeqpgfgll hlesqarnlp parphlgevh
      241 adlaaaaalr errrgsgpdn lgaprlcrpa ctdypqerrr gggpgrlgal liclcavfee
      301 tssrralfiq rlsscsakav vwpfvaeqqq pvivaererk irdsppyaaa agsskveghr
      361 vhgrrrssyp rg
//
LOCUS       XP_077511941             401 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122734 [Amblyomma americanum].
ACCESSION   XP_077511941
VERSION     XP_077511941.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655815.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..401
                     /product="uncharacterized protein LOC144122734"
                     /calculated_mol_wt=45701
     Region          160..>229
                     /region_name="CwlO1"
                     /note="Uncharacterized N-terminal coiled-coil domain of
                     peptidoglycan hydrolase CwlO [Function unknown]; COG3883"
                     /db_xref="CDD:443091"
     CDS             1..401
                     /gene="LOC144122734"
                     /coded_by="XM_077655815.1:1..1206"
                     /db_xref="GeneID:144122734"
ORIGIN      
        1 mrftdnpfll lvqlflalld hagdrsmqlp srmthgsdta dvrppdqlha ptwdhrwilq
       61 sattcadhym hlgqvatppd waiychptra aatghgsatr mrftdnpfll lvqvrkrygv
      121 cirsnnpfll vlpcprllfd vtvslrklll lggdieanpg pdtaqiltql qeiasdikei
      181 kekrladidn kldalarlek qvtscqeqva qmsevierle eriddldnrs rrcnlivygl
      241 seseeetket lrervnkdiv qdkldldava ierihrlgqp agnkirpvim klldyrekes
      301 ilkrgfklkn sdlsigedfs rkirdirkkl wdsakpnrdn gdkvslsfnk lyingqafvw
      361 dsenavkvpf gekndkattr esqndnvssp rmtrrransk k
//
LOCUS       XP_077511942             213 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122782 [Amblyomma americanum].
ACCESSION   XP_077511942
VERSION     XP_077511942.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655816.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..213
                     /product="uncharacterized protein LOC144122782"
                     /calculated_mol_wt=23622
     CDS             1..213
                     /gene="LOC144122782"
                     /coded_by="XM_077655816.1:1..642"
                     /db_xref="GeneID:144122782"
ORIGIN      
        1 marpnrviqf ilsssqpnkq fyfrrpfrps ssllrlpspl rdniaafgrg ssilqlifil
       61 ryrqpfaate ltgrpskfen hpdfvlsvcn yvrspgdaav lrhsrwltrh kgeletpara
      121 akrrvaapdd maefqqpang nrdldtnatq dnradedsva lqssanqiad vatssprdvt
      181 taqedtaelq etengikvht gqiavvksah ltr
//
LOCUS       XP_077511948             336 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122825 [Amblyomma americanum].
ACCESSION   XP_077511948
VERSION     XP_077511948.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655822.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 26% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..336
                     /product="uncharacterized protein LOC144122825"
                     /calculated_mol_wt=38310
     Region          74..162
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     CDS             1..336
                     /gene="LOC144122825"
                     /coded_by="XM_077655822.1:1..1011"
                     /db_xref="GeneID:144122825"
ORIGIN      
        1 mtlvevlgst gllpavsqvi apstptpatl hnpkvpvcfr gnafedveew ldqfervakf
       61 nqwsanqklc niyfallnna rtwfknhets lstwndfrrq lvdtfscpsr renalrllev
      121 riqklnesfa mfaadmarlf rradpdmteq rklrhlmhgv keqvlaglgr nppqsvsefv
      181 kdaiaieral qgrcrlydrl fngvptaaaa ltatdrttlr efireielee lqritatsvq
      241 spqavsvavr keqryalsps lpyneshpvd naaaivnqtt chnhappsst srqqrrffqe
      301 pqwappgrsq prrtdmwrsy drrplcffcg qpghvy
//
LOCUS       XP_077511954             378 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122834 [Amblyomma americanum].
ACCESSION   XP_077511954
VERSION     XP_077511954.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655828.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 37% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..378
                     /product="uncharacterized protein LOC144122834"
                     /calculated_mol_wt=41322
     Region          125..366
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..378
                     /gene="LOC144122834"
                     /coded_by="XM_077655828.1:1..1137"
                     /db_xref="GeneID:144122834"
ORIGIN      
        1 mptfyhlaaa qslvaaeirq srpkvpkrqc ildgvcrlpd sceyagidet pdssssstgc
       61 mddyptsisl qsslqgcsda dstsprlqss lqscsdvdft cpllqnslqg nsskevfcaa
      121 deaalhkria evedelkshe rklsicqrqk naalqeksal krrtchflan dqlksmerst
      181 mrgtpwstst iekglkirls cgsrgynvvr eigtplpser tlqrhleglk flpgilheil
      241 pslsakvnlm kdyekhavli ldemqltpgi aydqgtetvi gkptillsng tlpdnvmtth
      301 glvfmlpggt trwkqtvayh ltsnsfcsnt vkhgildiik scesigikid aivcdmgggn
      361 qapwkadsft swqtlpic
//
LOCUS       XP_077511960             210 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122844 [Amblyomma americanum].
ACCESSION   XP_077511960
VERSION     XP_077511960.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655834.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..210
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..210
                     /product="uncharacterized protein LOC144122844"
                     /calculated_mol_wt=22909
     CDS             1..210
                     /gene="LOC144122844"
                     /coded_by="XM_077655834.1:284..916"
                     /db_xref="GeneID:144122844"
ORIGIN      
        1 mhervpvssp mlrslrgtar iagmrpslvf ppaqagtykc vrvvechrgl lvtadmevif
       61 vkfelhhapn gdedayqgim gkclsregtp lpkmdvpdvt edqqsstect khriereqql
      121 vgrhrggtpr cclgssagaa fptgavtsae aalpprclps vrelrmrlpg lrssrswgrk
      181 parrirrhlp waptagscss ytrlsspses
//
LOCUS       XP_077511965             263 aa            linear   INV 25-NOV-2025
DEFINITION  FK506-binding protein 15-like [Amblyomma americanum].
ACCESSION   XP_077511965
VERSION     XP_077511965.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655839.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..263
                     /product="FK506-binding protein 15-like"
                     /calculated_mol_wt=28622
     Region          90..180
                     /region_name="FkpA"
                     /note="FKBP-type peptidyl-prolyl cis-trans isomerase
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG0545"
                     /db_xref="CDD:440311"
     CDS             1..263
                     /gene="LOC144122853"
                     /coded_by="XM_077655839.1:1..792"
                     /db_xref="GeneID:144122853"
ORIGIN      
        1 mmkyklilyk ekqqqvavaa inaqfvirlf gnnflsfade krcnwsvkfg seeavvsfak
       61 qaviakalsl ggsvtelnyy icqdlvvpge gdgdslvhgs savikytawl ltsehslkkv
      121 fdttesdepq kikigrgkhv kgleegllgc kknsrrllvl ppsfgygskg lgttippdsv
      181 lvydvsvssv skpkpaakds tprsstpvsa aavdadkrea rssshgsatq lhrvlphhhf
      241 hhearraqrf vasfhvpvrl qvv
//
LOCUS       XP_077511971             157 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122861 [Amblyomma americanum].
ACCESSION   XP_077511971
VERSION     XP_077511971.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655845.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 23% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..157
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..157
                     /product="uncharacterized protein LOC144122861"
                     /calculated_mol_wt=16749
     CDS             1..157
                     /gene="LOC144122861"
                     /coded_by="XM_077655845.1:1..474"
                     /db_xref="GeneID:144122861"
ORIGIN      
        1 mslggdnavd nglgssltam gsretdgitg nvgistssgg qcsrlpvvpd lfspdstnyr
       61 elfhqdllef yhnsvrfivt avapvevwds attsplfasp pplltrarvs pvvrlvprhc
      121 lsmvmecsee atcstihgtt alreelfadp vlhvyra
//
LOCUS       XP_077511976             184 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122871 [Amblyomma americanum].
ACCESSION   XP_077511976
VERSION     XP_077511976.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655850.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 20% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..184
                     /product="uncharacterized protein LOC144122871"
                     /calculated_mol_wt=20646
     CDS             1..184
                     /gene="LOC144122871"
                     /coded_by="XM_077655850.1:1..555"
                     /db_xref="GeneID:144122871"
ORIGIN      
        1 mlelleeppa kkprtswvsp clqrrkqqga yenlmrelal edsenyrrwi rmdtatfeel
       61 lgevrphitk kdtpfreaiq agerlaitlr ylatgnsems lqfafyvahn tisgiisevs
      121 qaiyrvlkpk vlkaptchde wlqiaqgfqe lwqfphciga ldgkhvvitp ppgsgaashg
      181 tcgc
//
LOCUS       XP_077511981             748 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122882 [Amblyomma americanum].
ACCESSION   XP_077511981
VERSION     XP_077511981.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655855.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 11% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..748
                     /product="uncharacterized protein LOC144122882"
                     /calculated_mol_wt=82921
     Region          <405..720
                     /region_name="CitT"
                     /note="Di- and tricarboxylate antiporter [Carbohydrate
                     transport and metabolism]; COG0471"
                     /db_xref="CDD:440239"
     CDS             1..748
                     /gene="LOC144122882"
                     /coded_by="XM_077655855.1:155..2401"
                     /db_xref="GeneID:144122882"
ORIGIN      
        1 msqgssnerr nadgrpstvr rrqppeitit ttnnadsvtf fgmwspvilf piiligtnea
       61 rcaycvcllv allltdalsp qviallpfvl fldrsvddhp lyfkipmqil helamvlmva
      121 svdatsllcr talyavslfg trvrtllclc ltgavlftll mngtaavlim aaiadnmvql
      181 lqndlvqvfq qralfekatv glsslrrrll qdilwprrfg drepattpag nvpspalsas
      241 sdettmrliq klssaqqgar hgadietipk lanpkyansw vldflidtnv addqlvnhem
      301 srasapvstd saeespprhs ppkqptvpnk ssllslprqa grrkktsile dgqvvipgqs
      361 spehkpvkgi npyhrvqlpp tfqryllqqi iawkkeryhi ihnqllmgvv vasslssmls
      421 rvqgsvqrdv igylesrfqr simsnaiwwi mlfpaemvgl aafwlrlkcn flsiydaped
      481 qqaqiaihll ienlsrdigl frhpelvptt vlltwvmahs lnvldwrgpy nfvdyhehld
      541 fdylgvilvf sipwvqcylg rpvdfrkvar rlpwgaffly lsslqlgfvt kefglaawit
      601 krmtsiptpn lllsqstlav csallteltg ddattsllmp ivvelavsnq chplyfaipv
      661 lvgastsvif pvgsmalall ysitdlgard mmvaglmtkv asiaitllta ntvgyyifew
      721 nvappwldgm stsatatnrs hdidpqlm
//
LOCUS       XP_077511997             254 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122898 [Amblyomma americanum].
ACCESSION   XP_077511997
VERSION     XP_077511997.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655871.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..254
                     /product="uncharacterized protein LOC144122898"
                     /calculated_mol_wt=28787
     Region          113..>213
                     /region_name="SRPBCC"
                     /note="START/RHO_alpha_C/PITP/Bet_v1/CoxG/CalC (SRPBCC)
                     ligand-binding domain superfamily; cl14643"
                     /db_xref="CDD:472699"
     CDS             1..254
                     /gene="LOC144122898"
                     /coded_by="XM_077655871.1:1..765"
                     /db_xref="GeneID:144122898"
ORIGIN      
        1 mnsatvqrpr hepsftacsy laprfrklsf aappgtggsn atperpapda rykkrltvia
       61 despplvaas ryyrfflrkr nakangwlsf vavclagaav lyqvevdqdr wvasqaitlk
      121 asagdafrfv tsseymskwl pfvssvread gkqmsvgkey ratcelpilg elgmsyrvve
      181 yapqrrvave seswlrprle lrfepgdsqc rmhvlvsfrr rsylfqytls pvlhfvsnqq
      241 lqrslfllrm vfpq
//
LOCUS       XP_077512006             348 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122917 [Amblyomma americanum].
ACCESSION   XP_077512006
VERSION     XP_077512006.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655880.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 35% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..348
                     /product="uncharacterized protein LOC144122917"
                     /calculated_mol_wt=39219
     Region          5..82
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          116..166
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          195..>305
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..348
                     /gene="LOC144122917"
                     /coded_by="XM_077655880.1:1..1047"
                     /db_xref="GeneID:144122917"
ORIGIN      
        1 mathcsvpws kqrgpkypgg nkvsyhrfpr ddtlyktwii aikrdegkyf kvakctrvcs
       61 sfgndrfkys nedfldytgi psyqhflalm tyinpgkngs nvlrnagikd sggrrrklta
      121 enelflvllr lrlglfehdl ahrfgtqsta sricvswinf myirlcklpl wvsravldgt
      181 mtaafstypt trvildatei kcevpsslsl qsssyftyka sntfkrlivv spnglvtfvs
      241 elytgsisdr eavlksgfln lkfdkndsvm adkgfliqdl ltsknvklni ppflhrnals
      301 eqeeapppwr iiafaagfrp varefllssl prslvevlpp apfklptc
//
LOCUS       XP_077512018             279 aa            linear   INV 25-NOV-2025
DEFINITION  RYamide receptor-like [Amblyomma americanum].
ACCESSION   XP_077512018
VERSION     XP_077512018.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655892.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..279
                     /product="RYamide receptor-like"
                     /calculated_mol_wt=32275
     Region          <32..223
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          50..66
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410628"
     Region          96..118
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410628"
     Region          149..171
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410628"
     Region          192..217
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410628"
     CDS             1..279
                     /gene="LOC144122935"
                     /coded_by="XM_077655892.1:1..840"
                     /db_xref="GeneID:144122935"
ORIGIN      
        1 msllrkctss keeqsegerl stalraqldt wyyaimspls frwtrkrgph iilviwlvaa
       61 llssvqfvhg ratpftwgdg tyydchenwd ekggkvytav iftvtfltpm lvltftyssi
      121 gwkmwrhtsp gnadiqrdqq qltakmkvvk mlatvvlmfa vcwlpihlmn lilyfdraam
      181 qpdtaeqeyl yiaaffschw fsmansfvnp iiycfmsdnf radvmqlilk rsltgldwar
      241 ttkrwsqqqq qqqqqkqhke lratysmnga lpgvatphr
//
LOCUS       XP_077512029             200 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144122955 [Amblyomma americanum].
ACCESSION   XP_077512029
VERSION     XP_077512029.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655903.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..200
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..200
                     /product="uncharacterized protein LOC144122955"
                     /calculated_mol_wt=22376
     Region          35..>188
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(89..94,135..136,171,173..174)
                     /site_type="active"
                     /db_xref="CDD:238822"
     Site            order(89..94,135,173)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238822"
     Site            136
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238822"
     CDS             1..200
                     /gene="LOC144122955"
                     /coded_by="XM_077655903.1:1..603"
                     /db_xref="GeneID:144122955"
ORIGIN      
        1 msiadapvll plvqlciepr acgqasskip kdralksrkt klgivfdafs heqgssslnd
       61 hlekgpclla dlvrllirfr lhqialtadi ekallqisvr kedrdslrfl wfnglpskde
      121 pdpeveewrm krvpfgtkas pfllnailqy hlktveepkk rtaallvesf yvddlllrad
      181 tekdaeeifv qandilgacg
//
LOCUS       XP_077512083             446 aa            linear   INV 25-NOV-2025
DEFINITION  monocarboxylate transporter 12-like [Amblyomma americanum].
ACCESSION   XP_077512083
VERSION     XP_077512083.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655957.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..446
                     /product="monocarboxylate transporter 12-like"
                     /calculated_mol_wt=47849
     Region          23..427
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(27..28,31..32,35,70,121..122,124..126,129,148,
                     151..152,155,258,261..262,265..267,270,297,301,352..353,
                     357,361,377,380..381,384..385,388)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:349949"
     CDS             1..446
                     /gene="LOC144123031"
                     /coded_by="XM_077655957.1:1..1341"
                     /db_xref="GeneID:144123031"
ORIGIN      
        1 mvkgaaerss ivfdscwtlp plsalsamlt sliikngali yvaiidefgv shesanwppt
       61 vhvlmahlag lvvsllqkkl ctnhialags vlyfiavvas avapdiiwma vtlgvfvglg
      121 ygiimlslsi daalyfdkyr gtasgvkytg mtlaplvfpl alsaliqgyg lhgtllivsa
      181 vafntlpvam lmskprpsee dsdkndlksg algqnyvaak largqgvlvt gssdvkessg
      241 disnffrnpf lyffvatfti seytvntfem tvldyalekg tprneaepii tyvaaaeifg
      301 pltvpflwdr atlalctlva lcltvtatsl aamphtssfa yvgmsavttg lscgcvvtlk
      361 pvllsdhfgv hmlslcwgms giamlplsfg gplllgvfrd tmgsydnlyr mlpglciifa
      421 gtlfafafye rrtrrrreev qnlhch
//
LOCUS       XP_077512096             560 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome P450 4C1-like [Amblyomma americanum].
ACCESSION   XP_077512096
VERSION     XP_077512096.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655970.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..560
                     /product="cytochrome P450 4C1-like"
                     /calculated_mol_wt=63504
     Region          98..551
                     /region_name="CYP4"
                     /note="cytochrome P450 family 4; cd20628"
                     /db_xref="CDD:410721"
     Site            order(128,146..147,154,158,165,361,364..365,368,430,434,
                     495..497,501..505,508..509,513)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410721"
     Site            order(147,363..364,368,433,435)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410721"
     CDS             1..560
                     /gene="LOC144123054"
                     /coded_by="XM_077655970.1:123..1805"
                     /db_xref="GeneID:144123054"
ORIGIN      
        1 mssmalvaaq wlwlwnscra faaalvafgv cklllwwwsq qrlyralqsi pgpggrwpvl
       61 fapryffhsl stnprragst veffqwirgl seihrstgif kiyaglepvv flcraetaep
      121 llsstsnlkk svlyknmhpw lgsgglitsc gerwrshrkl ltpafhfrvl enflpvineq
      181 gdrliddlrr langdyvnlf ptlskcalat icetamglkv hspertsavs ayekdlelai
      241 klfmkrvmqp wtwidavyga tekgamfdkv vnrlhcfttk vivdrqeada arraaarsgd
      301 veeerpglra svgkvmstpa vadaqgalgg aerpafldll seyyrrgvfs iedmrqevdt
      361 fmfaghdtsa qtltwtlfal ainpeiqrkv hmeldsvfaq qstrnmtksd vasltyldrv
      421 iketmrifti vpwvgrslte plkignytvp egctcyvfmy gvhrdpthyr dpevfdpdrf
      481 lpdkcsrshp fafmpfsagp rncigqkfam lelkvllakv lrsfsvsscn hrddllfdad
      541 illrtrrpir irlhprraae
//
LOCUS       XP_077512103             561 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome P450 4V2-like [Amblyomma americanum].
ACCESSION   XP_077512103
VERSION     XP_077512103.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655977.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..561
                     /product="cytochrome P450 4V2-like"
                     /calculated_mol_wt=64833
     Region          98..535
                     /region_name="CYP4"
                     /note="cytochrome P450 family 4; cd20628"
                     /db_xref="CDD:410721"
     Site            order(129,147..148,155,159,166,345,348..349,352,414,418,
                     479..481,485..489,492..493,497)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410721"
     Site            order(148,347..348,352,417,419)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410721"
     CDS             1..561
                     /gene="LOC144123073"
                     /coded_by="XM_077655977.1:1..1686"
                     /db_xref="GeneID:144123073"
ORIGIN      
        1 mfkdvlsgfs lvrtkfqtpw elaalvtlay gawalvvrpv vrwlrkwqal rplpgpcdgv
       61 pfwytlrtyw sksrsnsikd atsvffqiic dvceqyrgtt fkaylgmtpi vvlhspdavq
      121 tlltsktnlr kplvynfiss wlgdhnilta vgdswrfkrr lmtpafhfkv ledyvstfny
      181 ngtllvervd kltakqpgep ipafrivqnc aldvitkvlm gvdlgsqidt tdtsfanhfn
      241 mlmfligvrv frpwmwhdsl ydrtlegrly hqsiremeky tlavlerrkg klqqiaeele
      301 svpqdyavtg deeerdsviv drflkahlqd srysidevkk didcllfagt dtttsavgwa
      361 fymlglhpka lakvhqeldd ifcgdtvrdi tgddlkrmky lemcfkeslr lfppvpiigr
      421 vleedltidg ykipkgvtcf inmyslhrnp eyykdpdmyi perfltpevr nrhpfsyipf
      481 sggpknclgq rfahmeaktl lakvlrkytm estrpvsqlr ityelilkar gglriwfrer
      541 traeraakit dledkhtnyg l
//
LOCUS       XP_077512112             237 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123080 [Amblyomma americanum].
ACCESSION   XP_077512112
VERSION     XP_077512112.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077655986.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..237
                     /product="uncharacterized protein LOC144123080"
                     /calculated_mol_wt=27321
     CDS             1..237
                     /gene="LOC144123080"
                     /coded_by="XM_077655986.1:1..714"
                     /db_xref="GeneID:144123080"
ORIGIN      
        1 mqinvsktrt itftinnqlq nhcysvnsip ienastvkyl gvhfssdltw nnhidaivsk
       61 sskalgfirr hlhqadpatk playttlahs kieyasliwn pnqlylihkl esiqnkatrl
      121 itknysrhss ikaikvslkl pslekrrila llshfhrlyh shssfasyyi rpahrifprl
      181 dhalkiepmf arthlfshsp lflavhhwnk lpgytvftln hdafvqklkf llqinsd
//
LOCUS       XP_077512145             435 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123114 [Amblyomma americanum].
ACCESSION   XP_077512145
VERSION     XP_077512145.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656019.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..435
                     /product="uncharacterized protein LOC144123114"
                     /calculated_mol_wt=46180
     Region          351..>434
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     CDS             1..435
                     /gene="LOC144123114"
                     /coded_by="XM_077656019.1:357..1664"
                     /db_xref="GeneID:144123114"
ORIGIN      
        1 mppkpfavvk fpmeddslav vpvgwlshdw qhchwpkrra aevirlaree adpsapgcew
       61 frcpvavvtt crsyknaekk akkyemssav essaisgneg pdgpevrlps ppprlpsppp
      121 rlpspppqlp saprlispps llshsmeaat paganctagc passastagd ledveeatsd
      181 ptmlkvlrll leirqqqrei lqrlsrleqa rqeprtqsps ppvsslpplc pqlpafsied
      241 feaaeaavrd drvaaalkkq llrlggnnlk evaanvmgav mgvavqrlfs lrgrkgkrpf
      301 vgtklcrvat daicerqgvd ilaaqgfigr wlpgacdrgg crkrrfaqal eppeshaqap
      361 pvnpcagsap cpgapsascp atppgmails psgvipssap pphsllltps hpppklcpfp
      421 cppphsllvt pshlt
//
LOCUS       XP_077512160             463 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123148 [Amblyomma americanum].
ACCESSION   XP_077512160
VERSION     XP_077512160.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656034.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..463
                     /product="uncharacterized protein LOC144123148"
                     /calculated_mol_wt=50515
     Region          <64..>207
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..463
                     /gene="LOC144123148"
                     /coded_by="XM_077656034.1:1..1392"
                     /db_xref="GeneID:144123148"
ORIGIN      
        1 meekksvecs lvaynvpkeh waqlvfpvla grvaylstrf taeqhrdydk lkavvldelk
       61 lsaaeyqrrl sgatkrreet wkafvtrvqs ylnfylksrc vssfdgllkl lvadqlkagl
      121 seeahkyvkl regekwlnad eiaallqtye eaagkgsaqr kadsfkgfdt ppkprteapp
      181 tqkhgaergd siaavrpgge kkkvsrsggc frcgswrhvv ancpfdrenp adrrenpprd
      241 drltarvstq alqstrpalt dikircreav igaivdtgad iivvreslvp eefvsphgti
      301 dlvsafgekv eaklavvpla lcrgaplien vddatpvlca ltdkltqadc lisadaweql
      361 hdsgktdseg lvqavgtlsp paedrpdrsg pdvgtepala fadetlpqqt vdsnevepsq
      421 aggtaelsga qkfraeqhed dtlrqawqna kqgkagmsiv egv
//
LOCUS       XP_077512193             139 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123189 [Amblyomma americanum].
ACCESSION   XP_077512193
VERSION     XP_077512193.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656067.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 21% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..139
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..139
                     /product="uncharacterized protein LOC144123189"
                     /calculated_mol_wt=14661
     CDS             1..139
                     /gene="LOC144123189"
                     /coded_by="XM_077656067.1:245..664"
                     /db_xref="GeneID:144123189"
ORIGIN      
        1 msqslrnrrs eeaqaliaqr ttdakamfep stctgayasq asaafseqtn lkgtasqaea
       61 pavqnnhgaa lassptklty trnqlqdaqt tclgaappep ahnqswedeg mqallatapg
      121 rhsivsalai dsliwfhlh
//
LOCUS       XP_077512210             244 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123208 [Amblyomma americanum].
ACCESSION   XP_077512210
VERSION     XP_077512210.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656084.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 14% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..244
                     /product="uncharacterized protein LOC144123208"
                     /calculated_mol_wt=25675
     Region          <20..138
                     /region_name="PHA03378"
                     /note="EBNA-3B; Provisional"
                     /db_xref="CDD:223065"
     CDS             1..244
                     /gene="LOC144123208"
                     /coded_by="XM_077656084.1:154..888"
                     /db_xref="GeneID:144123208"
ORIGIN      
        1 mrplrrsisl sslhtnklqa arpsklpspa avrrqplgpn paggrgrfrq qtrgvatraa
       61 ppttplgprp afgvrrplrr slsassvqag kaalpcklps paavgrqplg qnpaggrgrf
      121 wqqargalsr pagpstephf qaaspllver skgcttampt grcgdtssal snvkplaggh
      181 itdgrsegal arpadeelle egrdldikqq atgsevprtd kapslleklf klseledelr
      241 ssfs
//
LOCUS       XP_077512224             339 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123228 [Amblyomma americanum].
ACCESSION   XP_077512224
VERSION     XP_077512224.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656098.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..339
                     /product="uncharacterized protein LOC144123228"
                     /calculated_mol_wt=36785
     Region          81..207
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     Site            order(84..87,116..118,121,146,185,203)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260008"
     Region          <267..303
                     /region_name="zf-RVT"
                     /note="zinc-binding in reverse transcriptase; pfam13966"
                     /db_xref="CDD:433612"
     CDS             1..339
                     /gene="LOC144123228"
                     /coded_by="XM_077656098.1:1..1020"
                     /db_xref="GeneID:144123228"
ORIGIN      
        1 mnatlnfqst sscahkdpva sktcrifhpy ltkladfyqr lslsietail gvrsekntpp
       61 calqqetaal lheeysgrve iytdgsvlda gasvaaacai pslgitrqcr lpfsasstta
      121 evaalhlaad hiletpdlsp avvlsdsraa latlqradht lpmyaslesk lrrvrnmcrd
      181 vslqwlpshv giqgneqadq lakqahadst pvctatssfd varqnirari vaqhpddrma
      241 agnaprpirs sdfepidrsi llkmrigcvs taermhrlrg atsplckdcg aietldhlls
      301 kcpallkhrn nlqacyrrla lpcdsaqqll fpagpreit
//
LOCUS       XP_077512230             113 aa            linear   INV 25-NOV-2025
DEFINITION  BEN domain-containing protein 5-like [Amblyomma americanum].
ACCESSION   XP_077512230
VERSION     XP_077512230.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656104.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..113
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..113
                     /product="BEN domain-containing protein 5-like"
                     /calculated_mol_wt=12510
     Region          28..103
                     /region_name="BEN"
                     /note="BEN domain; cl46346"
                     /db_xref="CDD:480686"
     CDS             1..113
                     /gene="LOC144123240"
                     /coded_by="XM_077656104.1:1..342"
                     /db_xref="GeneID:144123240"
ORIGIN      
        1 mkvqlgngvh vsadvwaalm mqtadaafck rlavalwgvk vlakrsvfgk qsnkdiakgk
       61 tmvhpplspm klvgmssafa hflkekecpe eelgkrhkql vryfaqkfad lrr
//
LOCUS       XP_077512246             164 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123259 [Amblyomma americanum].
ACCESSION   XP_077512246
VERSION     XP_077512246.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656120.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..164
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..164
                     /product="uncharacterized protein LOC144123259"
                     /calculated_mol_wt=17461
     CDS             1..164
                     /gene="LOC144123259"
                     /coded_by="XM_077656120.1:5..499"
                     /db_xref="GeneID:144123259"
ORIGIN      
        1 mmrrraicin pscpdrptdq chptgsglql amnddlgqga dccrersrni atrvtrpagl
       61 ppvaevanrf vasvrvinla igekgadqyy rhnyhkgrlg sekpvcgnap craqpsavpp
      121 atgtpttqss ksfpvvqeid lgpthhrlcg ansarrslgc hglp
//
LOCUS       XP_077512251             164 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123267 [Amblyomma americanum].
ACCESSION   XP_077512251
VERSION     XP_077512251.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656125.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..164
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..164
                     /product="uncharacterized protein LOC144123267"
                     /calculated_mol_wt=17461
     CDS             1..164
                     /gene="LOC144123267"
                     /coded_by="XM_077656125.1:5..499"
                     /db_xref="GeneID:144123267"
ORIGIN      
        1 mmrrraicin pscpdrptdq chptgsglql amnddlgqga dccrersrni atrvtrpagl
       61 ppvaevanrf vasvrvinla igekgadqyy rhnyhkgrlg sekpvcgnap craqpsavpp
      121 atgtpttqss ksfpvvqeid lgpthhrlcg ansarrslgc hglp
//
LOCUS       XP_077512260             513 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123277 [Amblyomma americanum].
ACCESSION   XP_077512260
VERSION     XP_077512260.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656134.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 35% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..513
                     /product="uncharacterized protein LOC144123277"
                     /calculated_mol_wt=56988
     Region          373..>438
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(387,389,391,413..415)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133137"
     Site            387..389
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133137"
     Site            387
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133137"
     Site            order(413..416,420..423)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133137"
     CDS             1..513
                     /gene="LOC144123277"
                     /coded_by="XM_077656134.1:1..1542"
                     /db_xref="GeneID:144123277"
ORIGIN      
        1 mdqnegatme ddfsvesrlq addqgqtlsl gatgtdtekm virrmelkve melrmreies
       61 rerlalkeme lglsngrmsp lvpgqsderc igsdsisqca kvlkgyrmpg dadvplwfee
      121 veklfttfnv pdssrvhlil paltervryl lrnldraect dyeavktaal eelkliptey
      181 lerfekaakr kgetwaqlas rvktylmfyi qarevdskea ltylmvsdhi kaslspegie
      241 yvrlregesw lkpkeiskal qtfeqakgke raarqitvpv lqtgqkqgnv ekpavkchqc
      301 hglghvarnc prvtaneprq qqayghrrrm qkvtrvdetg vkdaavnqga altaevgvpm
      361 qnsraaasrl qfvelvcgdv ctealldtgs emmiiresil pqartepsgt vklvsafgrs
      421 iqaklatvpl rishtgtiad dqkigivcal tneltegvhc llssedwqll ekhsdraftt
      481 ststavsmaa nqeptdaanq ncealcmmll piq
//
LOCUS       XP_077512270             374 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123288 [Amblyomma americanum].
ACCESSION   XP_077512270
VERSION     XP_077512270.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656144.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 32% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..374
                     /product="uncharacterized protein LOC144123288"
                     /calculated_mol_wt=40839
     Region          103..>172
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     Region          334..>374
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     CDS             1..374
                     /gene="LOC144123288"
                     /coded_by="XM_077656144.1:1..1125"
                     /db_xref="GeneID:144123288"
ORIGIN      
        1 msgraesaaq raavsgvtee gstpggsapd asallqdatr liqsisgalq aslvaskhsr
       61 pavkvavpty rgyadtvsvt dfieslthyq aavglddsem ltrivpvalv eraaqwyrls
      121 grrattldef kaalrleflp vdyqrrirre lelrtqasee slleyvrtme elfqtaepsa
      181 sndervervv rqahptfaay lrggrfrdld dlaaeakriq gdilamrtyr ppppaseave
      241 prcawtgafp yarhdspaqa vyavgadtrq ewklserald pftfhrrags aaaaascypn
      301 ksrtsaqgre qrdtfpkrpe grsagctkqe eafprrgntp aravrcfqcg elghisrlct
      361 rgpvqqgngv ggqa
//
LOCUS       XP_077512306             590 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier family 22 member 7-like [Amblyomma americanum].
ACCESSION   XP_077512306
VERSION     XP_077512306.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656180.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..590
                     /product="solute carrier family 22 member 7-like"
                     /calculated_mol_wt=63733
     Region          41..482
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..590
                     /gene="LOC144123327"
                     /coded_by="XM_077656180.1:1..1773"
                     /db_xref="GeneID:144123327"
ORIGIN      
        1 mdqpgpaamk qpglaaldqp alaigqeatg eeepavpfge glyqllalfn smlsgiaflc
       61 hlfsvrltah vmdhwcrqpp efanlsaaew kelaipraed gayshcrtcr rdppdggset
      121 vlcdswdfdl seygdnivse wqlvchrrrl ldlalyttfl asvlwlpmmg laadrvgrki
      181 vvyisipall itgfassvtg sfqfyvalrt vvsasivsvm tvafvvlyea tpagrrtlyc
      241 ftalalaqtf avvpfavvsv fklswkivyl lvmvptsllv itlytmdesy pwllatwrtk
      301 daerlaldaa rmngvshevc rasmshsyrg gnaeiaagrs ahsifsaelr rrtliiscfw
      361 aainfsysqa nlsdifplhp aaevagilll gpvhvvlcpa iryiglrrvm vgsalifsvv
      421 slflaatyrm qtvlsgnivv valrlmgslt tslgsflava aypttarctg lafafavgrl
      481 agscgeilfh yvsnerkdfp vaamsismal vtmaaeqhlp dvdagvaaav dsahsprigv
      541 tssgvramqd tldpmargpa grprgrrhhk rdsapeapvq atcipkglsh
//
LOCUS       XP_077512316             213 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123334 [Amblyomma americanum].
ACCESSION   XP_077512316
VERSION     XP_077512316.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656190.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 30% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..213
                     /product="uncharacterized protein LOC144123334"
                     /calculated_mol_wt=24160
     CDS             1..213
                     /gene="LOC144123334"
                     /coded_by="XM_077656190.1:243..884"
                     /db_xref="GeneID:144123334"
ORIGIN      
        1 mwwvryhisd dlallrevvs lnpfadqsqw gtiaenvtia tgrdfwtrsv kerlllmlrk
       61 flhnerqvlk qsgteegyte rdqllhqagd lskefgrslr pstkrvpkrp sctatarstd
      121 rtstqdgrva aamqfyeepa dpaaeenehi hcyhnltadr nddatadptd drvddhtddi
      181 iddpiddgnq aphrrvvkel lpppkflvad dgl
//
LOCUS       XP_077512329             145 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123358 [Amblyomma americanum].
ACCESSION   XP_077512329
VERSION     XP_077512329.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656203.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 18% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..145
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..145
                     /product="uncharacterized protein LOC144123358"
                     /calculated_mol_wt=16473
     CDS             1..145
                     /gene="LOC144123358"
                     /coded_by="XM_077656203.1:69..506"
                     /db_xref="GeneID:144123358"
ORIGIN      
        1 mepllayvly kdgekavvpv slikdycpks tkdlaknklv ywrdkdapdg gdedyypgdi
       61 eelaataeel akklnkrrir wpkhvfddaa heaaqqsaqq vmsqkqkkaq kkeachkklr
      121 fsfeesssld edlmektald gkkkk
//
LOCUS       XP_077512339             872 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123367 [Amblyomma americanum].
ACCESSION   XP_077512339
VERSION     XP_077512339.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656213.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..872
                     /product="uncharacterized protein LOC144123367"
                     /calculated_mol_wt=97835
     CDS             1..872
                     /gene="LOC144123367"
                     /coded_by="XM_077656213.1:1..2619"
                     /db_xref="GeneID:144123367"
ORIGIN      
        1 mdgdvtkkrr kryfykdepf vvpkttlyrr qqevrlraqh casstsaand gdvngggvvg
       61 dllnlpsgeq paaeqaaqpa sptlsahdeq aggtsyddde dlfqtddfdr lgetpqdsss
      121 mpgetenddg ereflasdfv eleaavipgs tttkaaaiim imafvvthgl twealndllr
      181 lidglfgfkg dvlprskfvf rklwssrker lvkrffycdt cgsvpvsvpg mssmrcpdce
      241 vstelcvlka rghffiildl keqmkcllak skaalferlv nlkaviqqgg aallqditsg
      301 smaeelrksg kigwmdltit intdgspvfk sssssvwpiq flinelppcd rlkncliggl
      361 wfgqhpymrt fltkfveein qfgkitwkag htllssglhv lcccvdapar asvinmvqfn
      421 glfgcpwcyn caefhegaqr ymsvtvgeer tpgemskdme faekikdavn glkgqsplnh
      481 lkhfnivfgh tveymhcvli gvtkcltdqw fdsansqqpf yigrpvtmak vdrrllaikp
      541 phsftrlprs lkdkchwkas ewrhwllfya lpcclgilpq rylnhfallv eaiftlllee
      601 ltlqqinhag rlledfvsrm palygarlmt fnvhqllhla kaaknfgplw ahsafvfetg
      661 ngrvlraits angapdqvie rmimlqqvdl amalctvqeq arnfictmlg hpltlnatrv
      721 gnsymfgacd pfpvlsteek nslmlsfgyv psleehfrfs frstvlhstr yerakksnst
      781 aicttendff vinrifqvte netrecillc tkvvcmesqh klpphimecf rslsgvitvl
      841 klkdihapcv liefaeeegl yicklpnfie rd
//
LOCUS       XP_077512348             336 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123376 [Amblyomma americanum].
ACCESSION   XP_077512348
VERSION     XP_077512348.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656222.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..336
                     /product="uncharacterized protein LOC144123376"
                     /calculated_mol_wt=37417
     Region          <302..>332
                     /region_name="YqaJ"
                     /note="YqaJ-like viral recombinase domain; cl09232"
                     /db_xref="CDD:447697"
     CDS             1..336
                     /gene="LOC144123376"
                     /coded_by="XM_077656222.1:45..1055"
                     /db_xref="GeneID:144123376"
ORIGIN      
        1 mapheplnag yyseligsir lryedklklc ddidpytlrp gvdtsadvsg fpeishgdiv
       61 tylvfsanfv tlenmkayka leshnyftsg wvkhlsakef qdgkvvllge vnhsqrlghk
      121 plqvwilckk dgavitahct cmtgageacs hvgaclfave tgikmmksrs ctqkdnmwlp
      181 ayvekvqykr lkdinftssk gkkrqldsns feagpvkera hvpplseqer tklygsiqda
      241 givpsifsvl pgydghfqnp vpmhdarlrn lyseemlahd ldvlvkkand vmpsiiisee
      301 tvkavealtr qqssssnwfl yragrvtasv mkrvnn
//
LOCUS       XP_077512357             405 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123388 [Amblyomma americanum].
ACCESSION   XP_077512357
VERSION     XP_077512357.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656231.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..405
                     /product="uncharacterized protein LOC144123388"
                     /calculated_mol_wt=42516
     CDS             1..405
                     /gene="LOC144123388"
                     /coded_by="XM_077656231.1:1..1218"
                     /db_xref="GeneID:144123388"
ORIGIN      
        1 mdkfvkvaeq fytdlyiiqs rsfknaekka kkyemssave ssaisgnegp dgpdvrlpsp
       61 pprlpspppr lpsppprlps pppqlpsppr lisppsllss hsmeaatpaa anctagcpag
      121 sastagdled veeatsdptm lkvlrlllei rqqqreilqr vsrleqarqe prtrspsppv
      181 sslpplcpql pafsiedfea aeaavrddrv aaalkkqllr lggnnlkeva anvmgavmgv
      241 avqrlfslrg rkgkrpfvgt klcrvatdai cerqgvdila aqgfigrwlp gacdrgggrk
      301 rryaqtlepp eshaqappan pcagsapcpg apsascpatp pgmailspsg vhpssappta
      361 sssrpatlhp ssapspappt asssrpatpr stlatwviap rahek
//
LOCUS       XP_077512365             361 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123399 [Amblyomma americanum].
ACCESSION   XP_077512365
VERSION     XP_077512365.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656239.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..361
                     /product="uncharacterized protein LOC144123399"
                     /calculated_mol_wt=40484
     Region          121..>179
                     /region_name="SWIM"
                     /note="Uncharacterized protein, contains SWIM-type Zn
                     finger domain [Function unknown]; COG4715"
                     /db_xref="CDD:443750"
     Region          <323..>353
                     /region_name="YqaJ"
                     /note="YqaJ-like viral recombinase domain; cl09232"
                     /db_xref="CDD:447697"
     CDS             1..361
                     /gene="LOC144123399"
                     /coded_by="XM_077656239.1:50..1135"
                     /db_xref="GeneID:144123399"
ORIGIN      
        1 mlsnswhrhr sqlffirfvp amapheplna gyyseligsi rlryedklnl cddidpytlr
       61 pgvdtsadvs gfpeishgdi vtylvfsanf vtlenmkayk aleshnyfts gwvkhlsake
      121 fqdgkvvllg evnhsqrlgh kplqvwilck kdgavitahc tcmagageac shvgaclfav
      181 etgikmmksr sctqkdnmwl payvekvqyk rlkdinftss kgkkrqldsn sfeagpvker
      241 ahvpplseqe rtklygsiqd agivpsifsv lpgydghfqn pvpmhdarlr nlyseemlah
      301 dldvlvkkan dvmpsiiise etvkavealt rqqssssnwf lyragrvtas vmkrqqlmht
      361 s
//
LOCUS       XP_077512383             542 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123429 [Amblyomma americanum].
ACCESSION   XP_077512383
VERSION     XP_077512383.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656257.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 25% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..542
                     /product="uncharacterized protein LOC144123429"
                     /calculated_mol_wt=59867
     CDS             1..542
                     /gene="LOC144123429"
                     /coded_by="XM_077656257.1:1..1629"
                     /db_xref="GeneID:144123429"
ORIGIN      
        1 mngdvtkkrr kryfykdepf vvpkttlyrr qqevrlraqh casstsaasa andgdvnggg
       61 vvgdllnlps geqpaaeqaa qpasptlsah deqaggssya ddedlfqtdd fdrlgetped
      121 sssmsreten ddgereflas dfveleaail pgstttkaaa iimimafvvt hgltwealnd
      181 llrlidglfg fkgdvlprsk fvfqklwssr kerlvkrffy cdtcgsvlvs vpgmssmrcp
      241 dcevstelcv lkarghffii ldmkeqmkcl lakskaalfe rlvnlkaviq qggaallqdi
      301 tsgsmaeelr ksgkigwmdl tipintdgsp vfksssssvw tiqflinelp pcdrlkncli
      361 gglwfgqhpy mrtfltkfve eisqfgkvtw kaghtvlssg lyvlcccvda parasvinmv
      421 qfnglfgcpw cyncaefheg vqrymsvtvg eertpgemsk diefaekikd avnglkgqsp
      481 lnhlkhfniv fghtveyrhc vligvtkclt dqwfdsansq qpfyiaasai fsaslcefvc
      541 sr
//
LOCUS       XP_077512392             266 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123439 [Amblyomma americanum].
ACCESSION   XP_077512392
VERSION     XP_077512392.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656266.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 46% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..266
                     /product="uncharacterized protein LOC144123439"
                     /calculated_mol_wt=29555
     Region          177..257
                     /region_name="BEN"
                     /note="The BEN domain is found in diverse animal proteins;
                     cl11158"
                     /db_xref="CDD:472128"
     CDS             1..266
                     /gene="LOC144123439"
                     /coded_by="XM_077656266.1:1..801"
                     /db_xref="GeneID:144123439"
ORIGIN      
        1 mepllayvly kdgekvvvpv slikdycpks tkdlaknklv ywrdkdapdg gdedyypgdi
       61 eelaataeel akklnkrrir wpkhvfddaa qqsaqvcnsd ipatrepelg seslgardvp
      121 geglaelaaa aptaveaaas sslipaeqgl pvqvhlacgv tlprvkrayl qtqpkdslfv
      181 remakalwgm nelynrsitg rpchrflhke gvselperra ltpeklkalr tafemhmekh
      241 pssktreerl rsinrslgdm lkvlkn
//
LOCUS       XP_077512419             144 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123469 [Amblyomma americanum].
ACCESSION   XP_077512419
VERSION     XP_077512419.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656293.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..144
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..144
                     /product="uncharacterized protein LOC144123469"
                     /calculated_mol_wt=15625
     CDS             1..144
                     /gene="LOC144123469"
                     /coded_by="XM_077656293.1:41..475"
                     /db_xref="GeneID:144123469"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpshrppfa altsvlccqr pesigpcvlp afgcvippdp
       61 fmlktkscme vvqatklrvi fardleqagk lvdgqwysls kasffsktkt iflgswamyl
      121 gtcgqdyagq hasvsskkka rnsk
//
LOCUS       XP_077512436             346 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123490 [Amblyomma americanum].
ACCESSION   XP_077512436
VERSION     XP_077512436.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656310.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..346
                     /product="uncharacterized protein LOC144123490"
                     /calculated_mol_wt=37444
     Region          221..309
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(235,237,239,259..261,298)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133137"
     Site            235..237
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133137"
     Site            235
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133137"
     Site            order(259..262,266..269)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133137"
     CDS             1..346
                     /gene="LOC144123490"
                     /coded_by="XM_077656310.1:1..1041"
                     /db_xref="GeneID:144123490"
ORIGIN      
        1 mpadgpssav sapslrppqf spadpqlwla qvnsqftigr ittqsqkshn vaasvppeia
       61 aevcdllitp pasapldtla aelikrtavs ehrrlqqlis snklgdrkpt qslrrlqqll
      121 gdkaatfgqa fllyqpptpp sslptrvglq slrddfhakm nvsatksphc rppalfrlpv
      181 arphladvvl phlmkpasag klpstpleas sgvtpdnsrl fficdhisgf rflvdtgaev
      241 svlpcsipss psrlpgpslq aanltsiaty gehalalnlg lrrvfrwvfl vvdvrypilr
      301 adfrrhynll vnmhtkclvd atanlsvqal rlreplasip sphqsi
//
LOCUS       XP_077512445             188 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123500 [Amblyomma americanum].
ACCESSION   XP_077512445
VERSION     XP_077512445.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656319.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 30% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..188
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..188
                     /product="uncharacterized protein LOC144123500"
                     /calculated_mol_wt=19636
     CDS             1..188
                     /gene="LOC144123500"
                     /coded_by="XM_077656319.1:1..567"
                     /db_xref="GeneID:144123500"
ORIGIN      
        1 msvvssvvpp stnattvaqp tkklrkevqk khdtelvdds eeqsgdadrn leggpgssrv
       61 aancngsshs alpptpttft ltaptqinph ssavpsterv isdsgqdefe kfsgeedina
      121 ddgncqleva maggedsiig stqddgktck vlgicsrcmq alvcgwtvvp giyysghlht
      181 kcsaalrl
//
LOCUS       XP_077512455             241 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123510 [Amblyomma americanum].
ACCESSION   XP_077512455
VERSION     XP_077512455.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656329.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..241
                     /product="uncharacterized protein LOC144123510"
                     /calculated_mol_wt=27232
     Region          80..240
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..241
                     /gene="LOC144123510"
                     /coded_by="XM_077656329.1:1..726"
                     /db_xref="GeneID:144123510"
ORIGIN      
        1 mpfedqillt lmrlrlglll ndlslgfgas taaisrifcf ivtklakfar eflvfwlprt
       61 airrtlpyvf eenysvtciv dcfeifidrp gqlhrrntty ssykshntak ilhviapsgf
      121 imflskpygg rasdrfitld sgflnhltqg devladrgfn iddllppsak vslpsfskgr
      181 pqicrsdvvs srrlarlrih veqsirrlkc frilkqfpas iftkkpfand vlvaiaglcn
      241 l
//
LOCUS       XP_077512466             108 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123520 [Amblyomma americanum].
ACCESSION   XP_077512466
VERSION     XP_077512466.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656340.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 26% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..108
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..108
                     /product="uncharacterized protein LOC144123520"
                     /calculated_mol_wt=12284
     CDS             1..108
                     /gene="LOC144123520"
                     /coded_by="XM_077656340.1:12..338"
                     /db_xref="GeneID:144123520"
ORIGIN      
        1 meqwrkrkea rkcarktdip prkkarleeq akgvakknae rellssqvgn speeskvahl
       61 eslvrdlkae lerckevigd kekemtnlrs lneslqkalc tkilqngm
//
LOCUS       XP_077512475             173 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123528 [Amblyomma americanum].
ACCESSION   XP_077512475
VERSION     XP_077512475.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656349.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..173
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..173
                     /product="uncharacterized protein LOC144123528"
                     /calculated_mol_wt=18693
     CDS             1..173
                     /gene="LOC144123528"
                     /coded_by="XM_077656349.1:196..717"
                     /db_xref="GeneID:144123528"
ORIGIN      
        1 mrcsqpvvke pssaarlaap srpvrrcllr srrhrarqap hplgpllqes trtprleaam
       61 ghaievkglg rrrhgqytvs plitrlattk varqtvanmq avtpvdasts sahfvlvgsg
      121 gpstlvrssm drepwwgsap ahpnascggr gasghlcdcg yflkrrplrp wkp
//
LOCUS       XP_077512495             348 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123549, partial [Amblyomma
            americanum].
ACCESSION   XP_077512495
VERSION     XP_077512495.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656369.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 29% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: incomplete on the carboxy end.
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..>348
                     /product="uncharacterized protein LOC144123549"
     CDS             1..348
                     /gene="LOC144123549"
                     /coded_by="XM_077656369.1:1..>1044"
                     /db_xref="GeneID:144123549"
ORIGIN      
        1 mrttvhdyvg nspdepgnki flvqekylwk lfqlctecla prtvrfeceg tllevygeca
       61 sghfvywcnq yvekqqpiln lqlcaavlfs gcnptatlrm lasigvpvvs nrtffatqrs
      121 ylwpaidrvw keeqksllqe lqgqqvnlag dgradspgfs akygtytlld verhkvlhfe
      181 vvqsndaggs crmeleglkq glaflesesi kvavlvtdrh tqikcflrnn kaaithefdv
      241 whmeskafqk lqdianskal lqdmprlstr hqtygledfh sllvhfapkt yhysylgmra
      301 rtqlavlhyn ensargqart mggtlrwcvr ypkaadgdpt acpvkepp
//
LOCUS       XP_077512539             174 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123603 [Amblyomma americanum].
ACCESSION   XP_077512539
VERSION     XP_077512539.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656413.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..174
                     /product="uncharacterized protein LOC144123603"
                     /calculated_mol_wt=19340
     CDS             1..174
                     /gene="LOC144123603"
                     /coded_by="XM_077656413.1:1339..1863"
                     /db_xref="GeneID:144123603"
ORIGIN      
        1 mrvehqgqql dailkrlsss apqssctted vlqqpfgdie sfeifdskls endslktrll
       61 hqlsglggts igsatrrmls lllspkvaik fswlgqkgkk ssrhwaikkn faeatrndve
      121 svikvwlrhs gdklkklelk aanvqqegef svkiaakhll acglllhywl veec
//
LOCUS       XP_077512554             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123623 [Amblyomma americanum].
ACCESSION   XP_077512554
VERSION     XP_077512554.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656428.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144123623"
                     /calculated_mol_wt=17165
     Region          109..143
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..158
                     /gene="LOC144123623"
                     /coded_by="XM_077656428.1:1..477"
                     /db_xref="GeneID:144123623"
ORIGIN      
        1 mprkrkkrrr adaagqwsvl drelgflddf lrprtrrstl ngaapaassa evevkreqpd
       61 edaevresaa vvaaaaappq pwplamrpaa vmphrlaagg aaaqalssdp eelfcpslaa
      121 rlkrllprer hmarmkmlqt lhdlesgenl gefalgdp
//
LOCUS       XP_077512561             544 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123634 [Amblyomma americanum].
ACCESSION   XP_077512561
VERSION     XP_077512561.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656435.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 16% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..544
                     /product="uncharacterized protein LOC144123634"
                     /calculated_mol_wt=60969
     Region          4..79
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          164..404
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..544
                     /gene="LOC144123634"
                     /coded_by="XM_077656435.1:173..1807"
                     /db_xref="GeneID:144123634"
ORIGIN      
        1 mtgccvplct gswkkglrtf rfprdperrk kweaqverek wkatdtskic erhfepdqye
       61 narqdgrrll kstavptlfd frpqpkrrkp pvrrrslads atsatesfqs tdvaspgsss
      121 lndqeldtge lcisdsteee eqeghcdild vdslstsemr kalqdmqkkn sqlkeslsva
      181 kkkmrtatrq aakletkvtm ltanlkylne dqktalerqd rtcrtwsadt vknalqlkfa
      241 cgstgydlli eqgyplpsrr tlcrrlqhls fkpgvlheil samqtkissm kdiekdcilf
      301 ldemeirsgi eldrgndtll gmitlpssdq pakhalvfmv ggtntrwkqv iafhytgayv
      361 sgdvlkdfvf hliklctdig lrvrcvtsdm rssnhamwrs lnlsssrnav tvssvphpfi
      421 snetlhfmpd pahvlknvrg hfvrkdpmhl sseivsrfns ptteitvehr cmgpfsgiyv
      481 agvevefegf grvslaalfa asyelpvqmp megmpvtlal wappgwvata leaplavtgg
      541 cvnr
//
LOCUS       XP_077512568             114 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123644 [Amblyomma americanum].
ACCESSION   XP_077512568
VERSION     XP_077512568.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656442.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..114
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..114
                     /product="uncharacterized protein LOC144123644"
                     /calculated_mol_wt=12335
     CDS             1..114
                     /gene="LOC144123644"
                     /coded_by="XM_077656442.1:13..357"
                     /db_xref="GeneID:144123644"
ORIGIN      
        1 mfrrssrarh svtasvqgrv lgfpgwcgyl vpedrlagrq gvdaammpgp mgvvaadvla
       61 ptlprqmvyv fptlgqfdpr cvrlvtldqd pataiacllg ffkvvceagy rhrl
//
LOCUS       XP_077512590             448 aa            linear   INV 25-NOV-2025
DEFINITION  dynactin subunit 4 [Amblyomma americanum].
ACCESSION   XP_077512590
VERSION     XP_077512590.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656464.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (DAPXPI010007753.1) annotated
            using gene prediction method: Gnomon, supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 359 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..448
                     /product="dynactin subunit 4"
                     /calculated_mol_wt=51071
     Region          21..>304
                     /region_name="Dynactin_p62"
                     /note="Dynactin p62 family; pfam05502"
                     /db_xref="CDD:428497"
     CDS             1..448
                     /gene="DCTN4-p62"
                     /coded_by="XM_077656464.1:11..1357"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 359 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144123676"
ORIGIN      
        1 madiftnerv lylcscdsrr pitriyfcrh csklrcsdcv shevdsnycs ncleympspe
       61 arlkknrcsn cfecpscght lsvrattiqv qtpedpsktv akkvyymacg lcrwstkdag
      121 lpdqalatgg wqefespwnk rvnalfehyr lmaqrdkmer errkssnrpg ylqfadrygv
      181 saavakkfag fispvskkee dlkkiedmkp avatdelval peeyftepvn iaqvcsigqr
      241 lsqpelqsey taflypkrkp llikrsqrcr ecehnlskpe fspssikfki qmaafhhipe
      301 ikirsvtafd igeecyvqls mynptphvth vtllplkqai egitakvllp mceftlpard
      361 dtaefddsse nifvddpsvv tfrkanslgf yirvipseee avivafrlkh dftnmvvqlq
      421 adhrepqvvw mthtvvvnlg arsyrpps
//
LOCUS       XP_077512595             136 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123684 [Amblyomma americanum].
ACCESSION   XP_077512595
VERSION     XP_077512595.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656469.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..136
                     /product="uncharacterized protein LOC144123684"
                     /calculated_mol_wt=13730
     CDS             1..136
                     /gene="LOC144123684"
                     /coded_by="XM_077656469.1:129..539"
                     /db_xref="GeneID:144123684"
ORIGIN      
        1 masslgatee vpdytvdgwp llneeptsat agdviarpal hihssgprsp sspstsllsg
       61 saipptghsa ltvcgttwlq dknrrcrgkl shgcapmpae pladlaatat tacartsgrl
      121 lgaldkcrgq amagpv
//
LOCUS       XP_077512602             195 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123691 [Amblyomma americanum].
ACCESSION   XP_077512602
VERSION     XP_077512602.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656476.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 14% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..195
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..195
                     /product="uncharacterized protein LOC144123691"
                     /calculated_mol_wt=20935
     CDS             1..195
                     /gene="LOC144123691"
                     /coded_by="XM_077656476.1:25..612"
                     /db_xref="GeneID:144123691"
ORIGIN      
        1 mgrghvvrvc lplfdypmdr rlasagqvpd rtldgwpsmn eeatsarpah rvclggrrsr
       61 sspnafplyg satthtghsa lamggttrlh dkdsgwrgkh shgsapttad ssadlpatat
      121 pptkawpvgy snikkpfcai dvvsavgavl iiwcawpttt avpaahrcrr lneplwatdd
      181 flravtaacp lrwre
//
LOCUS       XP_077512611             192 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123701 [Amblyomma americanum].
ACCESSION   XP_077512611
VERSION     XP_077512611.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656485.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..192
                     /product="uncharacterized protein LOC144123701"
                     /calculated_mol_wt=20483
     CDS             1..192
                     /gene="LOC144123701"
                     /coded_by="XM_077656485.1:1..579"
                     /db_xref="GeneID:144123701"
ORIGIN      
        1 mddesevtga aagrrktpds cwcgdgigfs vrhtrsddlt gvlvvrggrf pclhiglvgl
       61 hpdlrnfval dfvevdcvde sssyeltepy faprpnnrsg mqwmggvlcf nlnsianggl
      121 qesgvstllf iapvqpsmpp taevstskvm gyhnvppiag lrpslvfisc ctqlsvding
      181 tleltpprta im
//
LOCUS       XP_077512614              92 aa            linear   INV 25-NOV-2025
DEFINITION  dynactin subunit 4-like [Amblyomma americanum].
ACCESSION   XP_077512614
VERSION     XP_077512614.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656488.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..92
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..92
                     /product="dynactin subunit 4-like"
                     /calculated_mol_wt=10236
     CDS             1..92
                     /gene="LOC144123707"
                     /coded_by="XM_077656488.1:48..326"
                     /db_xref="GeneID:144123707"
ORIGIN      
        1 mlhshhipei kirsvtafdi geecyvqlsm ynptphvthv tllpleqaik gitakvllpm
       61 ceftlpardd taefddssen ifvddprqvs sd
//
LOCUS       XP_077512621             241 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123716 [Amblyomma americanum].
ACCESSION   XP_077512621
VERSION     XP_077512621.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656495.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..241
                     /product="uncharacterized protein LOC144123716"
                     /calculated_mol_wt=26273
     CDS             1..241
                     /gene="LOC144123716"
                     /coded_by="XM_077656495.1:1..726"
                     /db_xref="GeneID:144123716"
ORIGIN      
        1 madrlhqqvd davslrfpcl higlvglhpd lrnfvaldfv evdcvdesss yeltepyfap
       61 rpnnrsgmqw mggvlcfnln siangglqes gvstllfiap vqpsmpptae vstskvmgyh
      121 nvppiaglrp slvfiscctq lsvdindwkk srcgrnigrl lrsgdprppv hhaadeaagh
      181 hqlakgptpg rgcfpactrs wnspsprfhd lativgmrss lvllfcpsdi gmemkervrr
      241 s
//
LOCUS       XP_077512629             273 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123726 [Amblyomma americanum].
ACCESSION   XP_077512629
VERSION     XP_077512629.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656503.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..273
                     /product="uncharacterized protein LOC144123726"
                     /calculated_mol_wt=29463
     CDS             1..273
                     /gene="LOC144123726"
                     /coded_by="XM_077656503.1:1..822"
                     /db_xref="GeneID:144123726"
ORIGIN      
        1 mdesevtgaa agrrktpdsc wcgdgigfsv rhtrsddltg vlvvrggrfp clhiglvglr
       61 pdltnfvald fvevdcvdes ssyeltepyf aprpnnrsgm qwmggvlcfn lnsiangglq
      121 esgvstllfi apvqpsmppt aevstskvmg yhnvppiagl rpslvfiscc tqlsvdindw
      181 kksrcgrnig rllrsgdprp pvhhaadeaa ghhqlakgpt pgrgcfpact rswnspsprf
      241 hdlativgmr sslvllfcps digmemkerv rrs
//
LOCUS       XP_077512633             410 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123737 [Amblyomma americanum].
ACCESSION   XP_077512633
VERSION     XP_077512633.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656507.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 32% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..410
                     /product="uncharacterized protein LOC144123737"
                     /calculated_mol_wt=44485
     CDS             1..410
                     /gene="LOC144123737"
                     /coded_by="XM_077656507.1:1..1233"
                     /db_xref="GeneID:144123737"
ORIGIN      
        1 mpkdssptsa alpptvvcag alrqrdsavf sgtgdhdved wltsyervst hnkwddalkl
       61 nnvifyltdv anlwyrnhea gitswsafkq nfadvfgrsa vrklraeqll raraqlpgen
      121 ftsyiedvvd lcsradssmp erakidhilk gidddafqml laknpatvvn viaqcqsyde
      181 lrkrrlltrs plsnpeplga ltsdsdhstl lpqikqfvre evarqlslih pspdapsplg
      241 fslhrvireq vaealptlse vhsvpplsyt eaathysplh scggvldvrr mgcaasacgv
      301 ftqfacpasg tgptsgpsss slfatlkpla htgqsaymlf vrfrrprgtp lsspygrpss
      361 vcafslltpa fsgcstsgil cqrpasvydl sipvptspft vayapspyvr
//
LOCUS       XP_077512638             148 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123745 [Amblyomma americanum].
ACCESSION   XP_077512638
VERSION     XP_077512638.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656512.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="uncharacterized protein LOC144123745"
                     /calculated_mol_wt=16654
     CDS             1..148
                     /gene="LOC144123745"
                     /coded_by="XM_077656512.1:1..447"
                     /db_xref="GeneID:144123745"
ORIGIN      
        1 mpslvfiscc tqlsvdindw kksrcgrnig rllrsgdprp pvhhaadeaa ghhqlakgpt
       61 pgrgcfpact rswnspsprf hdlativgmr sslvllfchs digmemkerv rrsswplfas
      121 leehskrprg srdtwnlfis tfllyrir
//
LOCUS       XP_077512644             207 aa            linear   INV 25-NOV-2025
DEFINITION  dynactin subunit 4-like [Amblyomma americanum].
ACCESSION   XP_077512644
VERSION     XP_077512644.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656518.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..207
                     /product="dynactin subunit 4-like"
                     /calculated_mol_wt=23057
     Region          <29..>97
                     /region_name="Dynactin_p62"
                     /note="Dynactin p62 family; pfam05502"
                     /db_xref="CDD:428497"
     CDS             1..207
                     /gene="LOC144123753"
                     /coded_by="XM_077656518.1:2608..3231"
                     /db_xref="GeneID:144123753"
ORIGIN      
        1 mkpavatdel valpeeyfae pvnvaqvcsi gqrlsqpelq leytaflypk rkpllikrsq
       61 rcrecehnls kpefspssik fkiqmaafhh ipeikirsvt afdigeecyv qlsmynptph
      121 vthvtllple qaiggitakv llpmceftlp arddtsefdd ssenifvddp svvtfrkans
      181 lgfyirvips eeeavigqte ssptpas
//
LOCUS       XP_077512650             170 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123763 [Amblyomma americanum].
ACCESSION   XP_077512650
VERSION     XP_077512650.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656524.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 20% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..170
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..170
                     /product="uncharacterized protein LOC144123763"
                     /calculated_mol_wt=17674
     CDS             1..170
                     /gene="LOC144123763"
                     /coded_by="XM_077656524.1:53..565"
                     /db_xref="GeneID:144123763"
ORIGIN      
        1 masslgatee vpdctvdgwp llneeptsat agdviarpal hihssgprsp sspstsllsg
       61 saipptghsa ltvcgttwlq dknrrcrgkl shgcapmpae pladlaatat ppteawpvay
      121 ssakspfrap risvsaeapl ltmafawpts ttaaglaahr frrrirplws
//
LOCUS       XP_077512658             277 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123774 [Amblyomma americanum].
ACCESSION   XP_077512658
VERSION     XP_077512658.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656532.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 44% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..277
                     /product="uncharacterized protein LOC144123774"
                     /calculated_mol_wt=29684
     CDS             1..277
                     /gene="LOC144123774"
                     /coded_by="XM_077656532.1:1..834"
                     /db_xref="GeneID:144123774"
ORIGIN      
        1 mdrrlasagq vpdrtldgwp smneeatsar pahrvclggr rswsspnafs lygsatahtg
       61 hsalamggtt rlhdkdsgwr gkhshgsapt taetsadlpa tatppteawp vgysnikkpf
      121 caidivsavg avlmiwcawp tttavpaahh crrlseplwa tddffravta acplrwrgvm
      181 ddesevtgaa agrrktpdsc wcgdgigfsv rhtrsddltg vlvvrggrfp clhiglvglr
      241 pdlrnfvald fvevdcvdes ssyeltgisr vtiniiq
//
LOCUS       XP_077512671             307 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123792 [Amblyomma americanum].
ACCESSION   XP_077512671
VERSION     XP_077512671.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656545.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 15% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..307
                     /product="uncharacterized protein LOC144123792"
                     /calculated_mol_wt=34724
     CDS             1..307
                     /gene="LOC144123792"
                     /coded_by="XM_077656545.1:27..950"
                     /db_xref="GeneID:144123792"
ORIGIN      
        1 megpsappae spvrdkyqcp vcqaefvleq srrrhvrkqh peaasslscs akasyscdqc
       61 tnvfarrgql lqhheqnhgf vsrlsthqfc sysdfsawke meemkekvkf iassgpkrss
      121 sgvtqmyllc nrsemhvktg egkrqpklvg svkcnkhcfa tmavtqegen vtveyqaehy
      181 ghdksvkqqr ltstqknaia aklslgvpsk rilldahatg gdelnvlhtl nrhdvynvkr
      241 kfhigyeeqk dpdefvsmrm widelqetsa llyadkellt eqgkakfkma lmnstqkefl
      301 leyepgc
//
LOCUS       XP_077512677             246 aa            linear   INV 25-NOV-2025
DEFINITION  etoposide-induced protein 2.4 homolog [Amblyomma americanum].
ACCESSION   XP_077512677
VERSION     XP_077512677.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656551.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..246
                     /product="etoposide-induced protein 2.4 homolog"
                     /calculated_mol_wt=27337
     Region          <107..211
                     /region_name="EI24"
                     /note="Etoposide-induced protein 2.4 (EI24); pfam07264"
                     /db_xref="CDD:462129"
     CDS             1..246
                     /gene="LOC144123802"
                     /coded_by="XM_077656551.1:1..741"
                     /db_xref="GeneID:144123802"
ORIGIN      
        1 maddvdappe qqqrqgilqw lrnddalpls mstflmfvlc tlacdwtfll vakalacall
       61 fsgcedspdt wprmqalleh tsealwllpa lvltkvmscl sfqeladiay rahprvprtt
      121 mptlsdllan awfgvafptl fllqshllsm lapgvlgsfv stvhlsllys fsafeyvwfs
      181 rgwaphrrvs fvednwpyfv gfgmplafvt slsgslcirr ilfsiafpll fmsslaatpn
      241 sgtpfk
//
LOCUS       XP_077512689             163 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123820 [Amblyomma americanum].
ACCESSION   XP_077512689
VERSION     XP_077512689.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656563.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..163
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..163
                     /product="uncharacterized protein LOC144123820"
                     /calculated_mol_wt=17890
     CDS             1..163
                     /gene="LOC144123820"
                     /coded_by="XM_077656563.1:212..703"
                     /db_xref="GeneID:144123820"
ORIGIN      
        1 mgrfrstvtr ppndeglqkd tmveflpgad lhppakaeld nfddhqlpqe stpgssmslp
       61 prqqqlldda ktaspsfvsp speqvdsvpa madadssrwg paepavpqae pqrrlrsagn
      121 srwrlrtrcc fmglvcrhcr lrrlapgaal psslrwphsg lsw
//
LOCUS       XP_077512707             162 aa            linear   INV 25-NOV-2025
DEFINITION  Na(+)/citrate cotransporter-like [Amblyomma americanum].
ACCESSION   XP_077512707
VERSION     XP_077512707.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656581.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..162
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..162
                     /product="Na(+)/citrate cotransporter-like"
                     /calculated_mol_wt=17056
     Region          <1..124
                     /region_name="CitT"
                     /note="Di- and tricarboxylate antiporter [Carbohydrate
                     transport and metabolism]; COG0471"
                     /db_xref="CDD:440239"
     CDS             1..162
                     /gene="LOC144123848"
                     /coded_by="XM_077656581.1:1..489"
                     /db_xref="GeneID:144123848"
ORIGIN      
        1 mqefglaawi srrvlaipap nlplsqvalt acsallteit gdvstasllm pivvdvavin
       61 qchplyfaip vlvgastsii fpvgsmalal lysmtdqgar emmatglltk vstifivllt
      121 vntvgyyife wrdappwlea avtgtstnqs vgldstnptq lg
//
LOCUS       XP_077512714             231 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123858 [Amblyomma americanum].
ACCESSION   XP_077512714
VERSION     XP_077512714.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656588.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..231
                     /product="uncharacterized protein LOC144123858"
                     /calculated_mol_wt=25796
     Region          48..97
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          128..>215
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..231
                     /gene="LOC144123858"
                     /coded_by="XM_077656588.1:1..696"
                     /db_xref="GeneID:144123858"
ORIGIN      
        1 mvthevlrrs vletlsqddi vfytglpcpa fsrlaklisa ltnkptllsp edkllmclmr
       61 lrlgllyghl arifqvsvss vgnyikymle llsqimkrav vwlprkairn smpasfikng
      121 ygsttcildc tevalqrpkk lraraqtyss ykarntvkfl iaispsgcim fvskayagra
      181 sdkyktrdsh igdylvlgde imadrgftld eelevqgirl ntpaftkgmn s
//
LOCUS       XP_077512743             356 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123901 [Amblyomma americanum].
ACCESSION   XP_077512743
VERSION     XP_077512743.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656617.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 29% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..356
                     /product="uncharacterized protein LOC144123901"
                     /calculated_mol_wt=39765
     Region          <208..255
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..356
                     /gene="LOC144123901"
                     /coded_by="XM_077656617.1:1..1071"
                     /db_xref="GeneID:144123901"
ORIGIN      
        1 mrsepeviki hvatferrtv vysetrhvcr nnvtvatrpe rtlevkndvs qagkqetaaa
       61 eiptvadhql peyddqsdnw kayitkveay featgvsdsg kkrallvapl strtvqvlsg
      121 qvaprkpnsl tyeeavkvld ayfdpkrhei tesyrffnrc qlegsshtpa mhavlsefkd
      181 vfspelglid ghpvhlklke gampkfyhqp lvgllkadrp tpalaaariq rwalylggfr
      241 yqlqyspgpa neadgwplpl peatvyarnf grgekwtpgi vesttgsrmv sirtpagsvr
      301 rhvdqvrdre datppfskdq eagatlnpsd hlkpcgalhv kenqysdfti keeeml
//
LOCUS       XP_077512754             297 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123912 [Amblyomma americanum].
ACCESSION   XP_077512754
VERSION     XP_077512754.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656628.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 23% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..297
                     /product="uncharacterized protein LOC144123912"
                     /calculated_mol_wt=33222
     Region          63..154
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     CDS             1..297
                     /gene="LOC144123912"
                     /coded_by="XM_077656628.1:138..1031"
                     /db_xref="GeneID:144123912"
ORIGIN      
        1 mpnnaplapq ppaaplvcsg tprqrdpvvf sglgdsdven wlasyervst ynrwddamkl
       61 nnvifyladv anlwfrnhea diatwstfkt sftevfgrpg vrklraeqhl reraqqpget
      121 ftsyiedvvd lckrvnsdmt eadkiqnimk gidedafqml lakqpatvap vislcqsyee
      181 lrkqrvltrr tvqpadvlsa dvetaggqql rhdiqkfirq eiarqlalis gvrnhppals
      241 ptlqqtiraq pprvqadplp pafstnrqaa tshrsssprr rslspmrrra spsaaen
//
LOCUS       XP_077512765             358 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123923 [Amblyomma americanum].
ACCESSION   XP_077512765
VERSION     XP_077512765.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656639.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..358
                     /product="uncharacterized protein LOC144123923"
                     /calculated_mol_wt=39784
     Region          96..141
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          176..343
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..358
                     /gene="LOC144123923"
                     /coded_by="XM_077656639.1:1..1077"
                     /db_xref="GeneID:144123923"
ORIGIN      
        1 metetdlsmq nishlieeng rlkqrlasle eecsaakqka gelgnkhtnh aiitkevvmq
       61 saktvkfytg fvstamftaf lqfvlsawkp scatwldaet qlimvlmrlr lglltqdlsc
      121 rfgisnatvs sifhawldvl aanmtkliiw psrqtlqanr paafqdpffd gvtgiidcte
      181 vfiqrpilmt arsqtyshyk hhntvkilvv ispsgaitfv skawggrtpd keitlrsgll
      241 dkvkegdvfl vdrgfrcgem faargatllm psltknraql pgaevttsrk vssvrihver
      301 aiqrlkvfri fqtvlplnfv krtgdknlat idkvvvvcsa lvnlqtpiik nsqrrsas
//
LOCUS       XP_077512779             242 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123944 [Amblyomma americanum].
ACCESSION   XP_077512779
VERSION     XP_077512779.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656653.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 29% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..242
                     /product="uncharacterized protein LOC144123944"
                     /calculated_mol_wt=26278
     Region          21..>68
                     /region_name="PWWP"
                     /note="PWWP (Pro-Trp-Trp-Pro) domain; cl02554"
                     /db_xref="CDD:470613"
     Site            order(32,35,59)
                     /site_type="other"
                     /note="putative methyl-histone binding site [polypeptide
                     binding]"
                     /db_xref="CDD:438958"
     Site            33..36
                     /site_type="other"
                     /note="PWWP sequence motif"
                     /db_xref="CDD:438958"
     Region          67..>114
                     /region_name="PWWP"
                     /note="PWWP (Pro-Trp-Trp-Pro) domain; cl02554"
                     /db_xref="CDD:470613"
     Site            order(79,82,106)
                     /site_type="other"
                     /note="putative methyl-histone binding site [polypeptide
                     binding]"
                     /db_xref="CDD:438958"
     Site            80..83
                     /site_type="other"
                     /note="PWWP sequence motif"
                     /db_xref="CDD:438958"
     CDS             1..242
                     /gene="LOC144123944"
                     /coded_by="XM_077656653.1:1..729"
                     /db_xref="GeneID:144123944"
ORIGIN      
        1 masidqlerg kpktnlwnft vgalvwarlg sarrwpavvv masmcgqrpa apgclwvcwl
       61 aeyrisqvga lvwarlgsar rwpavvvmas mcgqrpaapg clwvcwlaey risqeiaars
      121 ctspcadpma waqatflahn aavaahtagp ggcpgvrpag lctplascgg hgvsicgqrp
      181 aapgrlwvcw laeyrisqld lkssswlsrr rcrrtrrdhq lrlpfscgrr cpatplqlpa
      241 cw
//
LOCUS       XP_077512786             147 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123952 [Amblyomma americanum].
ACCESSION   XP_077512786
VERSION     XP_077512786.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656660.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 11% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..147
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..147
                     /product="uncharacterized protein LOC144123952"
                     /calculated_mol_wt=16818
     CDS             1..147
                     /gene="LOC144123952"
                     /coded_by="XM_077656660.1:78..521"
                     /db_xref="GeneID:144123952"
ORIGIN      
        1 mwrllllsga systgavwsg itvctfgihp mwrllllsga systgavwsg itvctfaakq
       61 wcdlqgwfrp hdsyknwhss hvetaapkws tilnwssvew yhclhlwhss hvetaapkws
      121 tilnwssvew yhclhlwdcc cdfevvl
//
LOCUS       XP_077512794             660 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123963 [Amblyomma americanum].
ACCESSION   XP_077512794
VERSION     XP_077512794.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656668.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 30% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..660
                     /product="uncharacterized protein LOC144123963"
                     /calculated_mol_wt=74458
     Region          <178..262
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     Region          283..377
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(299,301,303,331..333,370)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133137"
     Site            299..301
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133137"
     Site            299
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133137"
     Site            order(331..334,338..341)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133137"
     Region          <466..490
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     CDS             1..660
                     /gene="LOC144123963"
                     /coded_by="XM_077656668.1:44..2026"
                     /db_xref="GeneID:144123963"
ORIGIN      
        1 mavgrigeyr lgtnaswdey verlemfcea nqitkeeqkr avllsscgee vyglivtlvk
       61 perptaatye eiktavrkhl hpkpselyar flfyrrnqaa eesvadyvta lrklaedcgf
      121 gsaqlpldvm mrdrfvcglr negvqqrlla ehsltfkvay dlattaeata kqqrdirkhg
      181 ryetdcqemv qatrakqdtp aegsscyrcn gkhaphlcrf rkatcfnckr vghiakacrs
      241 kddsrtarkt ayeqkkkgdk skgvyemsal fslcevneqe qkfmvqvtie gqevpmevds
      301 gascsivsed tfrvvernhg kiplqdsgtt ivtwskealp vlgqasvlve fkgrkaklpl
      361 lvvqkqgnsl igrnwfnplg iglrgiqqvn vedvpsrfpe vfrrdlpgfn gppvhielkd
      421 daqpvflksr pvplalkddv anevdrlvqq gvwepvsysn watplkaqlr vnkdkcefnq
      481 tsieflghri ddsgvhpsrs ktdaiqqapa paskkevqaf lgllnfyssf lkgrteaaep
      541 lyrlldrdhe wkwtgprwka asvvavrgpv syvvqlnnge rhhrhrnqlr sawtrlvvdp
      601 vtsedyhvrl pptarqpqpl llaqkqplgr vqakppkyrs sredqrvfdg lscvmalmas
//
LOCUS       XP_077512797            1389 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC144123973
            [Amblyomma americanum].
ACCESSION   XP_077512797
VERSION     XP_077512797.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656671.1
KEYWORDS    RefSeq; corrected model; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio   :: 34% of CDS bases
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1389
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC144123973"
                     /calculated_mol_wt=152084
     Region          <2..39
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          194..232
                     /region_name="Tudor_dTUD-like"
                     /note="Tudor domain found in Drosophila melanogaster
                     maternal protein Tudor (dTUD) and similar proteins;
                     cd20379"
                     /db_xref="CDD:410450"
     Site            order(202,209,225,228,230)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:410450"
     Region          405..570
                     /region_name="DDE_3"
                     /note="DDE superfamily endonuclease; cl46863"
                     /db_xref="CDD:481203"
     Region          <786..841
                     /region_name="Tudor_SF"
                     /note="Tudor domain superfamily; cl02573"
                     /db_xref="CDD:470623"
     Site            order(796,801,803,821,824,826)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:410449"
     Region          947..1063
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          1233..1338
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     CDS             1..1389
                     /gene="LOC144123973"
                     /coded_by="XM_077656671.1:3798..7967"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 2 bases in 1 codon"
                     /db_xref="GeneID:144123973"
ORIGIN      
        1 mvyfidygnv apvapenirp lpdlylqlpa qavrcrlhgv hpasaatgwt meaytmlatm
       61 fqagpvvana lklvddmhev elaskkggqs lnqqlvaegl aaslkaaasp aatsvvasaa
      121 epgmmphrrp mhavldlvki gdilsmqvtv sakgivwcla lmpdmpapll qvekalhdea
      181 tklggqsrah evtvgdyvas kstkdghwyr ayvtemtgsk htvryvdygn dednttifll
      241 askhkqvpaa avrlltsdpt sfrmgqllsf tvesidggta qgtvsseedr ksigglglvt
      301 whadlirggs aktsssspst passgwlhrf karhaivgki lsgestsadt tgsaswvdte
      361 vpkilahydp vdvynadeta lfyqmlpcrt lalkgekchg gkhsklrisv llcanmdgsd
      421 krvplvigrs kkprcfsnsr qleiqyasnq kawmtrdifr gwlqafdadm ktagrkvcvi
      481 ldncsahhie dlelsqvevk flppnctsli qpldqgiins vkcayrrrli dkllldlrle
      541 rptkvdlfqa lemlsaswns tskeifencf rkvgfktaea asseaalpal eeealderwq
      601 rlrsdgaipe gvelddylfg gscvlateev tvetivndvl gnsqddsege addecdiptt
      661 revadaidvl rrfasaeedq daiqslwaye rrlsptepap pksalakptp aepastpsaq
      721 papslqqsnv dakvlhvpqr klpsdrpdmv lvwrtpdglf vqqqsltgal nawvksqspe
      781 psttkyskag yvcalfseds iwyrgqvlse qspdgtylvl svdfgnrerm lasslrplpp
      841 sfaekplfaf cvvpqnvpte nsrlaallqq epftavqadt traglavvrl erkdgtcinn
      901 lisrsphkdp aqstvtgsap qeepqkppva vqtlvnvpdc plpkrpfdav vasvegnlvy
      961 iqpiirasgl lkftealank arrdpgykft emppvnqcvl avysdddqwy rarvvsades
     1021 gcmvrvqfld ygntetmsik lllpmqeela kepasvlavr legtpvlspw ageilsrepr
     1081 vvvqlvgsds gdgpplvrlm lkrkcinsma acmqqagisg falrrrtsfc qklpeayedk
     1141 laefrsyvir lrqqhgymlg qisnadetpv wfnmpssttw vngdnpktpt grlrrpplat
     1201 vatwasrawh slpndmvtpk kaylerplps ertkavithv dgdwlvylqq lslaselqav
     1261 mselnssvpt tplpapsvgd aacarypadn lwyrvcvlst pedgkckvsf vyfgnedfvp
     1321 lpdiralpdk mkdaqlfanc valqavktvs rdsasellnv evefevvdss qpckaklfsg
     1381 dqclndlnd
//
LOCUS       XP_077512804             321 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144123983 [Amblyomma americanum].
ACCESSION   XP_077512804
VERSION     XP_077512804.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656678.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 44% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..321
                     /product="uncharacterized protein LOC144123983"
                     /calculated_mol_wt=34689
     CDS             1..321
                     /gene="LOC144123983"
                     /coded_by="XM_077656678.1:1..966"
                     /db_xref="GeneID:144123983"
ORIGIN      
        1 msplynihls ppawctgide ipvggelspl saqerhpeqe avmplqqflp qpgpsnsqeg
       61 tlphpglplp pqfpwppqgp plpggptslp rqeetgpwgk pvfaaianpr ravmmddgtw
      121 yilvypafpe ndeclarals assarrvslr arklnldgne nspltttcsv qtilptftas
      181 slyvrylaly palgvpvvgs gfgtpgedgq vqlgngihls advwaalmmq tpdaafckrh
      241 avalwgvevl pkrsvvgkqs nkdiakgktm vhpplsamkl vgmssafahf fkekgcpeee
      301 lgkrhkqlvr ylaqkcadlr r
//
LOCUS       XP_077512877             380 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124082 [Amblyomma americanum].
ACCESSION   XP_077512877
VERSION     XP_077512877.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656751.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 39% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..380
                     /product="uncharacterized protein LOC144124082"
                     /calculated_mol_wt=41660
     CDS             1..380
                     /gene="LOC144124082"
                     /coded_by="XM_077656751.1:1..1143"
                     /db_xref="GeneID:144124082"
ORIGIN      
        1 maalayadgi vlvasslael rtnldvpvrh fpkqalklnp tktqyfgwhy daywlkwfdr
       61 rilelrlesa vvkpkapvwp taqlddtlgt tneagevhvg hgsyvpvaaw nlllalgses
      121 tfckqlavcl wgnktlaqsn ltgsdqelnl wrtcfhppfr cgpssssaag tdsprlgeeg
      181 slphggkrta tgapprrlrg ilrnrrealr dpasvvsgws rtlsspsaas trgsvvpcrw
      241 tsgsrqrsec skralwspsp anarewlclk naartgepgr hyrriqtfaa dtsevaplsq
      301 peryaefkrs cpgllsivwv sacclaamsh raaespassl qrlreggqpl ylptkarrre
      361 qhppdtlild acrgtrtgaw
//
LOCUS       XP_077512894            1012 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124102, partial [Amblyomma
            americanum].
ACCESSION   XP_077512894
VERSION     XP_077512894.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656768.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         <1..1012
                     /product="uncharacterized protein LOC144124102"
     CDS             1..1012
                     /gene="LOC144124102"
                     /coded_by="XM_077656768.1:<1..3039"
                     /db_xref="GeneID:144124102"
ORIGIN      
        1 tlththtlsh thshththth ththshthtl ththththth shthtlthth tlththshth
       61 tlshthtlth tlshthshth tthshthsht htlthththt htphththth ththththth
      121 ththththth ththththth thtlshthsl ththshthtl shthslthtl shthtlshth
      181 slthtlshth tlshthslth thshthttlt hthtlththt lshththsht hthtlththt
      241 lththtltht htlshthtls hthtlththt lththtlsht lslthtlsht hslthtlslt
      301 htlshtlsht htlshthslt htlshthtls hthtssptht lshthshtht lthtlshths
      361 lthtlshtls hthsltltls hsltlshtls htlshttrth trthtrthtr sltlshshtl
      421 thshshtltl thshthtlsh shtitlthsh thtlshshtl tlshthtlth thshthtlsh
      481 thtlthtlth tlthtlthtl thtlthtlth tlthtlthth thtltlshth tlthshtlth
      541 thtlthshth shtlshshtl thtltlshtl shtlthsltl shtltlshsh tltlshthtl
      601 tlshthsltl shtlthtlth tlthtlthtr thtrthtrth trsltlshsh tlthslshtl
      661 tlthththtl shshthtlsh thtshththt htlshthtlt lthththths hshthtlths
      721 hthtlshsht ltltpthsht ltphtthsht htlthshtlt lshshtlslt htlslththt
      781 htlshthsht htlshtlsht lslthslsht lshthtrsls hthshsltht hshtlshtht
      841 hthththsht htlththtlt hththththt htlththsth tlthtlthtl thtlthtlth
      901 tlthtlthtl thtlthtlth tlshthshtl shtlthtlsh thshtlthth htthtlshtl
      961 thththshth shthshthsh thshtltlsh shtltigdpd vfyanddatl as
//
LOCUS       XP_077512904             445 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124113 [Amblyomma americanum].
ACCESSION   XP_077512904
VERSION     XP_077512904.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656778.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 23% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..445
                     /product="uncharacterized protein LOC144124113"
                     /calculated_mol_wt=48668
     Region          174..262
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     CDS             1..445
                     /gene="LOC144124113"
                     /coded_by="XM_077656778.1:1..1338"
                     /db_xref="GeneID:144124113"
ORIGIN      
        1 mspgqpvlfl fdscetpsan eglgpniarr eprapplrnl rapgearraa adlrqprlap
       61 efsrrtglra parvraricc gplsggggrq agaltegsap dasallqdat rliqsisgal
      121 qasleaskhs rpavkvavpt yrgyadtvsv tdfieslthy qaavglddse mltrivpval
      181 veraaqwyrl sgrrattlde fkaalrrefl pvdyqrrmrr elelrtqald eslleyvrtm
      241 eelfqiaeps asnderverv vrqahptfaa ylrggrfrdl edlaaeakri qgdilamgay
      301 rppppaseav eprcawtgaf syarhdspaq avyavgadtr qewklseral dpftfhrras
      361 saaaaascyp nksrtssqgr eqrdtfpkrp egrsagctkq eeafprrgnt pargvrcfqc
      421 gelghisrlc trgpvqqgng vggqa
//
LOCUS       XP_077512924             436 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124134 [Amblyomma americanum].
ACCESSION   XP_077512924
VERSION     XP_077512924.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656798.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..436
                     /product="uncharacterized protein LOC144124134"
                     /calculated_mol_wt=49348
     CDS             1..436
                     /gene="LOC144124134"
                     /coded_by="XM_077656798.1:91..1401"
                     /db_xref="GeneID:144124134"
ORIGIN      
        1 mpplklwsgy htvkkignas tlsevvkqaq ekgvctsena kvflrdwtev eedffqevie
       61 hllleerifi vtdtapttep tlqghsgtqn msssnpeget aalqvdftfk vdtgsfpetv
      121 laclrpggpl lrpkdrrdlv qcivgqmvak cslkdrgvdg hllgrgydsl fqqmenrven
      181 mtrgkrqpsq vpdrkskkra nvsygclnwq pilnssgdaa qekvrflrqe glksareinl
      241 plalqymqet ygdqrdyins dnpvhqtrei kaqwpllfha rffyghadql lgkdvkaise
      301 edsnkkevly yyvqmelees rgnrgakeaa flpllaalfk ddigqlfrvf eegtsiterl
      361 helpctptvv algsffqnqc yiiceqqvlf septgfgeat rlaflayyvf nmsypvkaat
      421 tleflqreif dinptk
//
LOCUS       XP_077512941             306 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124154 [Amblyomma americanum].
ACCESSION   XP_077512941
VERSION     XP_077512941.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656815.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 37% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..306
                     /product="uncharacterized protein LOC144124154"
                     /calculated_mol_wt=34347
     CDS             1..306
                     /gene="LOC144124154"
                     /coded_by="XM_077656815.1:1..921"
                     /db_xref="GeneID:144124154"
ORIGIN      
        1 mddfylllsl mlcnlprgwg sklgtdwfqn hvndlleffp nvsepprvpl ysekqadais
       61 rfgeqwphtr aalcstllkt rfqgpmqqvq eyvfvgwrfa gmkhvylile flggrnscvl
      121 dvtpeqrarr drleaflcay qrlgadgpym nllnlaeaat tlrkhlglhv aaayalavle
      181 danwlqykgv psdlyermgl cappkdvlqr hrrltatsap wtaperaspp lphelaqqrt
      241 tatatlatss ppqgsqrggg rewdgsapnp htaierpqnm pplsprvahy rqlsqaprpl
      301 sfrrpi
//
LOCUS       XP_077512972             165 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124193 [Amblyomma americanum].
ACCESSION   XP_077512972
VERSION     XP_077512972.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656846.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..165
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..165
                     /product="uncharacterized protein LOC144124193"
                     /calculated_mol_wt=17987
     Region          <1..73
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            4
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     Region          107..>163
                     /region_name="RT_RNaseH_2"
                     /note="RNase H-like domain found in reverse transcriptase;
                     pfam17919"
                     /db_xref="CDD:465567"
     CDS             1..165
                     /gene="LOC144124193"
                     /coded_by="XM_077656846.1:1..498"
                     /db_xref="GeneID:144124193"
ORIGIN      
        1 mpfglcnaaq tfqrtineft rgldfvfayi ddlvasssgs ehevilrtlf hrldeyglvm
       61 nlnkflfgca sflkpltvll atkndfaapp ewtedtaatf aaakktlada tlliypvpga
      121 plclvtdass navstvleqh vsswqplgff sqklkppenk ystfg
//
LOCUS       XP_077513004             194 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124232 [Amblyomma americanum].
ACCESSION   XP_077513004
VERSION     XP_077513004.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656878.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..194
                     /product="uncharacterized protein LOC144124232"
                     /calculated_mol_wt=20946
     CDS             1..194
                     /gene="LOC144124232"
                     /coded_by="XM_077656878.1:95..679"
                     /db_xref="GeneID:144124232"
ORIGIN      
        1 mkktyadnhk pttaiagaea vpskqstntr kpsenatnpr kaatgagdly sppstrsqkd
       61 rckkemekve dsmseaskps kpkfdadtgr sdlkelrsap rakgarsspn isagpsaasl
      121 gherardkys fhegqgtakg paegavqnwq lrqiqvkvss pweqllkige avsgsshrsm
      181 trqtqlthyf sfaf
//
LOCUS       XP_077513040             429 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124273 [Amblyomma americanum].
ACCESSION   XP_077513040
VERSION     XP_077513040.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656914.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..429
                     /product="uncharacterized protein LOC144124273"
                     /calculated_mol_wt=46914
     Region          <190..>356
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          193..213
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          215..239
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          241..263
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          264..286
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          287..306
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..429
                     /gene="LOC144124273"
                     /coded_by="XM_077656914.1:1..1290"
                     /db_xref="GeneID:144124273"
ORIGIN      
        1 msaifafavv faaslhgvgr adgnieklft tflyrghati qmrrpkhdvf iteadiselr
       61 qflcllrqvq rgdildqcvf srppskvtrt qealvvnsqh kyppqgfpst lkslrieasq
      121 regrmdvvta degmgaggqv ladeyelrmt ggrddrsnva kcvaqvsdth tghffqgcim
      181 vqpgsdvlal anlsvlnlka dgiyalptem snlplrfltv senritelqp elfenrlssc
      241 llcldvsnnq ltvlpetlcn arklmslaag rnrlhklpdg lgdlpdlwml dltsnaltll
      301 pasllklswi siwgnplsst agqpmehtaa swqvplplte laaravvavg nlesiakdvp
      361 rrmlqvlrka tpcygcqrpv fpataasvtf rmelrgtvlp gggqlvwvpt hdgrvpvral
      421 icstrclph
//
LOCUS       XP_077513050             699 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124283 [Amblyomma americanum].
ACCESSION   XP_077513050
VERSION     XP_077513050.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656924.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..699
                     /product="uncharacterized protein LOC144124283"
                     /calculated_mol_wt=78284
     Region          463..545
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            466
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238180"
     CDS             1..699
                     /gene="LOC144124283"
                     /coded_by="XM_077656924.1:1..2100"
                     /db_xref="GeneID:144124283"
ORIGIN      
        1 mpatranpsg adsspadrpc trgrtvtvhr afrtlpkavg klgknpelpc qsrrpfpaee
       61 ipfavsgdgn ayawkllwgi ngmhmqraay aeprarhapg garstvfrpm gfrptlefin
      121 lhrsygqrai teicrftsvt traasfkwsl elqhdicktl glipqslrln rpvstsfgys
      181 ivakaerfll qarildckqt irklenkaff arcrlecqys avflsvqlha nfqaklsaar
      241 aeqshedely rlthqdrpah snvtlstvhs lyshkpdpal msvlslglnf kwhrpqtken
      301 ilyavenais kvepsrreea rtrtlgallr lrplqasstl ssvqcnaikq lqanadlvil
      361 padkgnatvl ldkvtyktkc wpcfrnvrld ncdmmvsfdv ktlfmsvstd lavevctaal
      421 esdatspgrs pidvpdlgrl rrfclsntyf tfdqvfykqv hgtamgasvs vtaanltmew
      481 peshalnsfs pqpkifvwyv ddcfaiikkd vlfdftahln steravqftv ekeengqlpf
      541 ldvlvkrsqs mlsskvyres thtgcyphfh sfhpashkrs vvaslflqan rictepedvk
      601 adrqgvrkdf secgyllpfv naverkllqp prsdsgsaak krgpvpyvpg fndalarilr
      661 sydaevshva akkllvnair ghkcglrviv ryqgtyqyp
//
LOCUS       XP_077513059             283 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124294 [Amblyomma americanum].
ACCESSION   XP_077513059
VERSION     XP_077513059.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656933.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 25% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..283
                     /product="uncharacterized protein LOC144124294"
                     /calculated_mol_wt=31519
     Region          <134..205
                     /region_name="MULE"
                     /note="MULE transposase domain; pfam10551"
                     /db_xref="CDD:463145"
     CDS             1..283
                     /gene="LOC144124294"
                     /coded_by="XM_077656933.1:45..896"
                     /db_xref="GeneID:144124294"
ORIGIN      
        1 mtpsqainhh esllavqpdg pqllasgfvn ptkravyymh dhwrtdkygp cgsplvklqe
       61 kmpqyaaqgi mpstdvytss kvddcwaalv vtpimrraqs lesaqelifv dstascdttr
      121 stvtvlltat kagavpiavl ihssqsregy slafqllkhc yptcfgnrea paafmsdssr
      181 pekdalrdvw psaqqllchf hilqaewrwi tsshhkvdkd drrrfmaafq kvlysktkee
      241 leeakaalcs lphqdyvqrv dsllkteeew vamyraagsa gtg
//
LOCUS       XP_077513078             284 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124314 [Amblyomma americanum].
ACCESSION   XP_077513078
VERSION     XP_077513078.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656952.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 30% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..284
                     /product="uncharacterized protein LOC144124314"
                     /calculated_mol_wt=30736
     CDS             1..284
                     /gene="LOC144124314"
                     /coded_by="XM_077656952.1:172..1026"
                     /db_xref="GeneID:144124314"
ORIGIN      
        1 mpenaaasir tlgsnlfevp sckedgkiye vwqdvgtctc ragqqgafck hqalvhsthg
       61 ggfpnapvls tqdrhqlawl algdkcqnpa ffrdfresaw dqpgsgsges gnepvqpqpq
      121 plvqdatslp qdtmeveeql avagpssnhq cpilgfsqda aevlknitee lwrqynlaad
      181 npfyltllqr dlallkrart epqvvamhla staakasslr rgriikvqpt glarrrpgvt
      241 rgaarvhagr plktagsrkt krlhslhlsv kdavphaksh gtgh
//
LOCUS       XP_077513114             163 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124355 [Amblyomma americanum].
ACCESSION   XP_077513114
VERSION     XP_077513114.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077656988.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 39% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..163
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..163
                     /product="uncharacterized protein LOC144124355"
                     /calculated_mol_wt=18068
     CDS             1..163
                     /gene="LOC144124355"
                     /coded_by="XM_077656988.1:1..492"
                     /db_xref="GeneID:144124355"
ORIGIN      
        1 msenerdcvl fldeleiwrg veldgssdcf lgkitlpesd qpanhalvfm nmthcvmstf
       61 sfpkatlaps pqpspqpsqq ltpepllqps prttpslfge rrrddeanev lrqtlyeqrr
      121 ladltqarnr qdaafqqsml epirevadav qasmgskscs sgt
//
LOCUS       XP_077513142             198 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124385 [Amblyomma americanum].
ACCESSION   XP_077513142
VERSION     XP_077513142.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657016.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..198
                     /product="uncharacterized protein LOC144124385"
                     /calculated_mol_wt=21699
     CDS             1..198
                     /gene="LOC144124385"
                     /coded_by="XM_077657016.1:1..597"
                     /db_xref="GeneID:144124385"
ORIGIN      
        1 malarmefrt inqrsptlqq tiqaqiaeal pllpqgsihc svyckrilqa fkkeplnkra
       61 ekdhvsqpaq tsasackpgr pssvpsknds hpcetalvki lwelelikve qarqaavlsk
      121 iaakviseaq sadavapddm pvypvktqee ldileastkd aatfaslvkt lghrlcraqf
      181 afqrtinstp qcmmllsy
//
LOCUS       XP_077513160             350 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124406 [Amblyomma americanum].
ACCESSION   XP_077513160
VERSION     XP_077513160.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657034.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..350
                     /product="uncharacterized protein LOC144124406"
                     /calculated_mol_wt=38921
     Region          102..147
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          183..341
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..350
                     /gene="LOC144124406"
                     /coded_by="XM_077657034.1:1..1053"
                     /db_xref="GeneID:144124406"
ORIGIN      
        1 mqqetqtdhe gagsgsfsvl iccaqgcdvs tqiahretrd cevqhkpavt stfsgpdhrt
       61 syfagydsie kssgaledlc ivnsvvfall lsmlsisrer kcdvskenrl liflmklklg
      121 isysslasff tinetsasrh fksvlkthvf atkqwffrpp trviqatmpt cfkahypgct
      181 miidctevrt erpptiqqqr alystykggy tlkflvgilp sgaicfhsna yggrmsdthi
      241 tvdsgfldll eagdliladk gfpgirtvlg trnavlvmpp flqgpqftaq evrdtynvaq
      301 vrihvdrmiq rikmhilnnr vpveliplmt dvfhmccvla nlqpplikck
//
LOCUS       XP_077513171             130 aa            linear   INV 25-NOV-2025
DEFINITION  peroxynitrite isomerase THAP4-like [Amblyomma americanum].
ACCESSION   XP_077513171
VERSION     XP_077513171.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657045.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 23% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..130
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..130
                     /product="peroxynitrite isomerase THAP4-like"
                     /calculated_mol_wt=14935
     Region          14..95
                     /region_name="THAP"
                     /note="The THAP domain is a putative DNA-binding domain
                     (DBD) and probably also binds a zinc ion; smart00980"
                     /db_xref="CDD:214951"
     CDS             1..130
                     /gene="LOC144124412"
                     /coded_by="XM_077657045.1:1..393"
                     /db_xref="GeneID:144124412"
ORIGIN      
        1 mdvgrtrkkt nrmyccakdc hsregngdvk lfrfparpfe strrlkwvva vrrvsgedfs
       61 nwspndntri cskhfvngek stiethpgyl ptiflpvyrk rsrdsagqls rfnrwmqrsa
      121 vpatagpqeq
//
LOCUS       XP_077513202             250 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124429 [Amblyomma americanum].
ACCESSION   XP_077513202
VERSION     XP_077513202.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657076.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..250
                     /product="uncharacterized protein LOC144124429"
                     /calculated_mol_wt=26723
     Region          5..119
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     CDS             1..250
                     /gene="LOC144124429"
                     /coded_by="XM_077657076.1:1..753"
                     /db_xref="GeneID:144124429"
ORIGIN      
        1 mdilgraaaa lvapqlgvtk tcrlsfpgss taaelaglhl aadlmaalpp gpvavlcdsr
       61 palqlltrsh dvvattsqlr arlhaledag rritlhwvpg hsgiegndla dasaksahad
      121 gspvvrtlhp drriaarkpf rrlsdqlprr drslllrlri gctwtpsrly vhgrasspac
      181 pscgdtgtlg hlllacpahd lprqrlesgy ralglrttte ddllfpvrnq lqahrvllsf
      241 lgesglslll
//
LOCUS       XP_077513234             205 aa            linear   INV 25-NOV-2025
DEFINITION  myb/SANT-like DNA-binding domain-containing protein 3 [Amblyomma
            americanum].
ACCESSION   XP_077513234
VERSION     XP_077513234.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657108.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 39% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..205
                     /product="myb/SANT-like DNA-binding domain-containing
                     protein 3"
                     /calculated_mol_wt=23033
     Region          8..85
                     /region_name="Myb_DNA-bind_5"
                     /note="Myb/SANT-like DNA-binding domain; pfam13873"
                     /db_xref="CDD:433544"
     CDS             1..205
                     /gene="LOC144124447"
                     /coded_by="XM_077657108.1:1..618"
                     /db_xref="GeneID:144124447"
ORIGIN      
        1 mektekgkin fsrverdlll dlvdkhkavl enkrtdavsv arkrkeweli etqfnsshnv
       61 sprtwlqlkk cwenwknkwr kakaddnrei fktgggsaap sqltedlqrv gsvashmgvr
      121 lanpfdsdrh qadpggesqp apsvaallsr tqdgsipgas dhrdklakra avhqlemenl
      181 klkndlkrsk killelqika vkgel
//
LOCUS       XP_077513245             236 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124451 [Amblyomma americanum].
ACCESSION   XP_077513245
VERSION     XP_077513245.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657119.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 37% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..236
                     /product="uncharacterized protein LOC144124451"
                     /calculated_mol_wt=24070
     CDS             1..236
                     /gene="LOC144124451"
                     /coded_by="XM_077657119.1:195..905"
                     /db_xref="GeneID:144124451"
ORIGIN      
        1 mvpeqavalg tpahvdaatn ivschlpvet slvmneamaw dtpahgallm plpyptatat
       61 ltmscqqtaa snlpavpata vsslanvata tpmascgisa asdllvnpam vvgtpadaqq
      121 lgraatkkpa alvsktlgvl vvgigfgtpg edgqtvstyv fvsdmpttgs tarpkvegve
      181 arggaarewc ppafahflke kgcteeelgk rhkqlvrylh ksvvicgane rgatal
//
LOCUS       XP_077513266             350 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124461 [Amblyomma americanum].
ACCESSION   XP_077513266
VERSION     XP_077513266.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657140.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..350
                     /product="uncharacterized protein LOC144124461"
                     /calculated_mol_wt=38598
     Region          7..103
                     /region_name="rve"
                     /note="Integrase core domain; pfam00665"
                     /db_xref="CDD:459897"
     CDS             1..350
                     /gene="LOC144124461"
                     /coded_by="XM_077657140.1:1..1053"
                     /db_xref="GeneID:144124461"
ORIGIN      
        1 mplvdtpfrr vavdiigplk pttrkgnryi mtlvdyatrf peavalpsie tervaeallq
       61 ifarvgvpge mlsdrgsnft sefmaevgrl lslrlqttsp yhpmanglvg gpqastgfsp
      121 fgllygrnvr gpltilkelw tgdkveetkt ayqyvvdlre rlettcqmah ealeeagery
      181 kryydrgsks ralqvgdqvl lllptehnkl tmkwkgpyva kgnkgevdyv vdvegnpktf
      241 hvnmlkkyfs rvpetdshvp vvaavasnie dgalvwplge qadhrdvaas kkltppqaqe
      301 lrflisrhke lfsdgpgytt wavcnlttti ivqahpgqav paslrsasgs
//
LOCUS       XP_077513329             456 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124495 [Amblyomma americanum].
ACCESSION   XP_077513329
VERSION     XP_077513329.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657203.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..456
                     /product="uncharacterized protein LOC144124495"
                     /calculated_mol_wt=48104
     CDS             1..456
                     /gene="LOC144124495"
                     /coded_by="XM_077657203.1:159..1529"
                     /db_xref="GeneID:144124495"
ORIGIN      
        1 mppkpfavvk fpmeddslav vpvgwlshdw qhchwpkrra aevirlaree adpsapgcew
       61 frcpvavvtt crsyknaekk akkyemssav essaisgneg pdgpdvrlps ppprlpsppp
      121 rlpsppprlp spppqlpspp rlisppslls shsmeaatpa ganctagcpa gsastagdle
      181 dveeatsdpt mlkvlrllle irqqqreilq rvsrleqarq eprtrspspp vsslpplcpq
      241 lpafsiedfe aaeaavrddr vaaalkkqll rlggnnlkev aanvmgavmg vavqrlfslr
      301 grkgkrpfvg tklcrvatda icerqgvdil aaqgfigrwl pgacdrgggr krryaqalep
      361 peshaqappa npcagsapcp gapsascpat ppgmailsps gvhpssappt asssrpatlh
      421 pssapspapp tasssrpatp rstlatwvia prahek
//
LOCUS       XP_077513366             436 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124516 [Amblyomma americanum].
ACCESSION   XP_077513366
VERSION     XP_077513366.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657240.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..436
                     /product="uncharacterized protein LOC144124516"
                     /calculated_mol_wt=47498
     Region          163..252
                     /region_name="retropepsin_like"
                     /note="Retropepsins; pepsin-like aspartate proteases;
                     cd00303"
                     /db_xref="CDD:133136"
     Site            order(177,179,181,205..207,245)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133136"
     Site            177..179
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133136"
     Site            177
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133136"
     Site            order(205..208,212..215)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133136"
     CDS             1..436
                     /gene="LOC144124516"
                     /coded_by="XM_077657240.1:1..1311"
                     /db_xref="GeneID:144124516"
ORIGIN      
        1 mgkrtydyis dfltvrytqi cagnlqpdnk ahytqmgaag plryvqrtrd hfalafstpv
       61 fafwvplclr ttwfldptsg klapatfgge aaerrsdakl ppphrptrpd verriaaqfs
      121 ptlhpnrpdv atpvvdqlpp phrlspspqp stgraegris ldipvlvdtk evsalvdtga
      181 dysilsgrla ttlkkvttpw egpdirtagg hvvaplgmca arvtvrgctf vatflvlrec
      241 srdvilgidf lrehgaiidl pnrlvtfstk lataepahqg sqsalrvaed nitvpprasi
      301 larvtcdepq rglrvvepnv slvltkgiav arglsylhdg wtevlitnfc nehrhllrgt
      361 aiayvdrvqe vaecfaceed ttggdaeald nvninpglss aekgqlqdll refracftts
      421 skaaqattsl fyfpyg
//
LOCUS       XP_077513401             234 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124531 [Amblyomma americanum].
ACCESSION   XP_077513401
VERSION     XP_077513401.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657275.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..234
                     /product="uncharacterized protein LOC144124531"
                     /calculated_mol_wt=26201
     CDS             1..234
                     /gene="LOC144124531"
                     /coded_by="XM_077657275.1:36..740"
                     /db_xref="GeneID:144124531"
ORIGIN      
        1 meleqrisnw lpaldkeive ltvqklqqcg veslehlkyv veedlqflkp isrrillerf
       61 hsaatpnppv slspndiqsp tstqcasfst pwevfppqlm kacqegkrpv ksdlnemema
      121 sdddlgdlps cpficakgwt fltansytic vdthpvlhas kpdeafkllf fahfafniqy
      181 qketslclef tqraiaginp argtkvqkng gkqhclsprv aalvtalkdy dffl
//
LOCUS       XP_077513410             689 aa            linear   INV 25-NOV-2025
DEFINITION  acetylcholinesterase-1-like [Amblyomma americanum].
ACCESSION   XP_077513410
VERSION     XP_077513410.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657284.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 45% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..689
                     /product="acetylcholinesterase-1-like"
                     /calculated_mol_wt=76782
     Region          157..643
                     /region_name="Abhydrolase"
                     /note="alpha/beta hydrolases; cl21494"
                     /db_xref="CDD:473884"
     Site            order(259..261,340..342,345,504,505..506,535,581,584)
                     /site_type="other"
                     /note="substrate binding pocket [chemical binding]"
                     /db_xref="CDD:238191"
     Site            order(341,482,580)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238191"
     CDS             1..689
                     /gene="LOC144124539"
                     /coded_by="XM_077657284.1:1..2070"
                     /db_xref="GeneID:144124539"
ORIGIN      
        1 msiesledld ppryfeerfe iasldsllpr psvpcydrwk lvadgstpls dagcscstrs
       61 gnvspcsqrq hrvpsewppv heqtqaddea yrsemqtprs qmgsseimqq cfsqhtqqyg
      121 hvgthstrnk illycfiftv iiliafvlil garaggdvsv ttpygrcwga rlhadgrevf
      181 hfrsvpfaes plgdrrfapp vplsgpgrsq rtdgcvcpqh qpllyslaft tlcedclhan
      241 iwtpvlgagr lpvlvlfhgl hftyggneqr lldgatlaar anlvvvvpny rlnlfgfftd
      301 ntsdapgnvg lldqrlaldw lrvsvssfgg dphsmvlhgy gsgaaslglq llspnphwlq
      361 qrarrlllqs gsplqpfkrn vivlfeqlgf llvelrctsm seakkclres svdtllrsmn
      421 rtryafaprf qtsflpcerv hdfsllragp ekklillgnv wgegvvlydm mrqlyskgre
      481 dsalwpairh dlldyglrqv drlwslyass dgdakytnlt gdgsdsergp maemlgdvfh
      541 vcplqyfaqe lsaaghqvfv yvfsakpsqs qwpeeqrpal yedlpflfga pfsepgptwe
      601 rllsarmidl ladfvhrgvl ptlddgtawp pykhlrpavv eigssglrml asgfrdgicr
      661 rlrphllgnr grtasiagcv rgndpfrre
//
LOCUS       XP_077513416             147 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124546 [Amblyomma americanum].
ACCESSION   XP_077513416
VERSION     XP_077513416.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657290.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 29% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..147
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..147
                     /product="uncharacterized protein LOC144124546"
                     /calculated_mol_wt=15944
     CDS             1..147
                     /gene="LOC144124546"
                     /coded_by="XM_077657290.1:59..502"
                     /db_xref="GeneID:144124546"
ORIGIN      
        1 mdetvddenv sfemlidsmq qeppspadde wsmtqaavse ppspppydsg cqpsalpsps
       61 splpaapstd hrlqtappns eatehhhqtp ppnshqaakp srhatqesvt rpcqhksqvv
      121 rrpqkkperr nrveengdgn spginsh
//
LOCUS       XP_077513466             220 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: pancreatic triacylglycerol lipase-like
            [Amblyomma americanum].
ACCESSION   XP_077513466
VERSION     XP_077513466.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657340.1
KEYWORDS    RefSeq; corrected model; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio   :: 5% of CDS bases
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..220
                     /product="LOW QUALITY PROTEIN: pancreatic triacylglycerol
                     lipase-like"
                     /calculated_mol_wt=23911
     Region          <3..198
                     /region_name="Abhydrolase"
                     /note="alpha/beta hydrolases; cl21494"
                     /db_xref="CDD:473884"
     Site            26..30
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238362"
     Site            order(28,142)
                     /site_type="other"
                     /note="catalytic triad"
                     /db_xref="CDD:238362"
     CDS             1..220
                     /gene="LOC144124580"
                     /coded_by="XM_077657340.1:1..663"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:144124580"
ORIGIN      
        1 mevvkvgasr lqetfggdpk smhiighslf ahvagyagan isslgrital dpaepyfekt
       61 ptvvllnqtd aefvdviqtd shpflaklwe iegmgmhdav ghvdfytigg khmpgcdtas
      121 rilklftqgv fkgvldivsc nhqravdyml esienqdclp layecpsfea dkraqcsdcg
      181 pdgagcaamg eraiqwsrfk gseprrmytv tdarsnfcgk
//
LOCUS       XP_077513481             466 aa            linear   INV 25-NOV-2025
DEFINITION  pancreatic lipase-related protein 2-like [Amblyomma americanum].
ACCESSION   XP_077513481
VERSION     XP_077513481.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657355.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..466
                     /product="pancreatic lipase-related protein 2-like"
                     /calculated_mol_wt=52375
     Region          58..348
                     /region_name="Pancreat_lipase_like"
                     /note="Pancreatic lipase-like enzymes. Lipases are
                     esterases that can hydrolyze long-chain acyl-triglycerides
                     into di- and monoglycerides, glycerol, and free fatty
                     acids at a water/lipid interface. A typical feature of
                     lipases is 'interfacial activation,' the...; cd00707"
                     /db_xref="CDD:238363"
     Site            176..180
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238363"
     Site            order(178,201,292)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238363"
     Site            order(266,279,289..290)
                     /site_type="active"
                     /note="active site flap/lid"
                     /db_xref="CDD:238363"
     CDS             1..466
                     /gene="LOC144124581"
                     /coded_by="XM_077657355.1:133..1533"
                     /db_xref="GeneID:144124581"
ORIGIN      
        1 mrwtavvlpi slallvanar dgekcygdlg cleldepfhd pvhrpvvytp adrnematrf
       61 llytwcsdpr kytelrwdsd ldeiraapyn asretkflvh gyidrtsfgp wmndmknayl
      121 khadvnvvvv dwsdanlgmy egavtnariv gaeialfvrk lqetfggdpk smhiighslg
      181 ahvagyagan itslgrttal dpaepyfekt ppvvrldqtd aefvdlihtd shpflaklwe
      241 segmgmydav ghvdfypngv kympgcdtas rifklfthgv fkgvrdivsc nhqravdyml
      301 esienqdclp layecpsfea ykraqcsdcg pdgagcavmg eraiqwsrfk gsdprrmytv
      361 tgarsnfcvh qyvvtltmck kvgpvsgrgh iylidrrqga qeikmtsrpm nfdleqnytf
      421 lvttpipise nskllieyks tlihflynvp llrvsvrqmt salter
//
LOCUS       XP_077513492             546 aa            linear   INV 25-NOV-2025
DEFINITION  putative transporter YutK [Amblyomma americanum].
ACCESSION   XP_077513492
VERSION     XP_077513492.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657366.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..546
                     /product="putative transporter YutK"
                     /calculated_mol_wt=58172
     Region          263..>527
                     /region_name="nupC"
                     /note="nucleoside transporter; TIGR00804"
                     /db_xref="CDD:273278"
     CDS             1..546
                     /gene="LOC144124585"
                     /coded_by="XM_077657366.1:1..1641"
                     /db_xref="GeneID:144124585"
ORIGIN      
        1 maaqegngnv lvpedacpag pkeeppaedg kmsttdhgpt stpcplqqgp leglvpvqqc
       61 yvmlppsrhq llpddtalpt dplpvwsdgv sarglhicps ryiidelvgm ptkaeesgrf
      121 kphlatnlgp lfalgtylvf ssyrasaqga dgwcsvrnla vvgaiivtvv plvgrvasda
      181 ldnwpefrek aeafvadlvc galseltlai hisvkhvsrs fvlwyvsava llewkalvpf
      241 ltglgselra taqlsssgld aifggfavgi nitsplqrtg fefrdvldve apfllrvlpr
      301 fffvafwinv vhcssplrtg fvsvfrmlrf csgttlteti ssvsnffvpm aeslllahpy
      361 msvmtpsevf taltcgfatw sanvvpelvd agvslndimt sailgslgcv cmsklvipev
      421 dkvkvktggf sidnsqpfwc vetlsselmv cltlaasmsg cliasvtfha aagtiiswia
      481 dsagvepiga daavgtmfrp lacvfglpwn htkvvsrvms lklllsepda atylkkhaev
      541 igcsnd
//
LOCUS       XP_077513504             313 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124592 [Amblyomma americanum].
ACCESSION   XP_077513504
VERSION     XP_077513504.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657378.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 52% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..313
                     /product="uncharacterized protein LOC144124592"
                     /calculated_mol_wt=35337
     Region          5..89
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..313
                     /gene="LOC144124592"
                     /coded_by="XM_077657378.1:1..942"
                     /db_xref="GeneID:144124592"
ORIGIN      
        1 mptqcvavgc snthsvpgis lhrfpkcdkl rklwvlalkr drwepskasv lcsahfkped
       61 fvrdidvaas lpcrqlkrll kpeaipsvfa hckpsiqnlr pafekrrrie mnmsrwsptg
      121 atatdvlsep kyivfesclv ellgpcrtcl aqcstevkrs gslvqvtaic pfghvwswqs
      181 qpslhrkaag nillaniivt drhsairall rkeypeilhl fdcwhmakgk kkkllaaskr
      241 rgvevlekwa raavnhlyws aassdghpeq ivpkwssltr hvinvhqhds sifprcvhgd
      301 lsedkrlwld egk
//
LOCUS       XP_077513526             347 aa            linear   INV 25-NOV-2025
DEFINITION  palmitoyltransferase ZDHHC15A-like [Amblyomma americanum].
ACCESSION   XP_077513526
VERSION     XP_077513526.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657400.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 25% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..347
                     /product="palmitoyltransferase ZDHHC15A-like"
                     /calculated_mol_wt=38877
     Region          141..259
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; pfam01529"
                     /db_xref="CDD:396215"
     CDS             1..347
                     /gene="LOC144124606"
                     /coded_by="XM_077657400.1:1..1044"
                     /db_xref="GeneID:144124606"
ORIGIN      
        1 mlpfdgrptd lksdgasaca earrtlaqmf awvtlllvlg lfmwayyayv lvfcrllmdr
       61 nsvamvsffg tgfhilfffc lwsylqttat sapaiprvys lsveeqiala cchddykrra
      121 vldalatqrg vltvgldgca rhceqcqlik pdrchhcsnc hrcvpkmdhh cpwfnncvhf
      181 anykfflltl iylvllsvfs vattgtyvvl swlgatdips svhmkfllvv gtvlafamgv
      241 flwthlsmvl snqttledmc gpifrnpeds fdigryenfv evfgprvwlw mlpvftsigd
      301 gvrfpsrlhp vpgslerlst faalrtpapd gpsllnhpvl dpkpsar
//
LOCUS       XP_077513548             212 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124620 [Amblyomma americanum].
ACCESSION   XP_077513548
VERSION     XP_077513548.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657422.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..212
                     /product="uncharacterized protein LOC144124620"
                     /calculated_mol_wt=23400
     Region          9..99
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..212
                     /gene="LOC144124620"
                     /coded_by="XM_077657422.1:286..924"
                     /db_xref="GeneID:144124620"
ORIGIN      
        1 maprrlnhcf apgcktgyvh vkgapkpslf svpkdealrr lweknlhrad kpledtsavc
       61 elhfepryvi rdyvhiiggk evriprgkpl lssdavptil pnlpayltkk avkerparkr
      121 ksescaspak rrpcsnsdtt vsytsaatia eqgaasstea gvepdadaef scplgqqepm
      181 llfdslqvps eqwtkhspie kslihyrgsc rr
//
LOCUS       XP_077513552             571 aa            linear   INV 25-NOV-2025
DEFINITION  luciferin 4-monooxygenase-like [Amblyomma americanum].
ACCESSION   XP_077513552
VERSION     XP_077513552.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657426.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..571
                     /product="luciferin 4-monooxygenase-like"
                     /calculated_mol_wt=62460
     Region          62..547
                     /region_name="AFD_class_I"
                     /note="Adenylate forming domain, Class I superfamily;
                     cl17068"
                     /db_xref="CDD:473059"
     Site            order(208,211..216,218..219)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341237"
     Site            order(211,327..328,350..355,433,445,448,459,547)
                     /site_type="other"
                     /note="AMP binding site [chemical binding]"
                     /db_xref="CDD:341237"
     Site            order(211,253..254,300,302..303,306,327..328,350..355,433,
                     445,448,456..459,528)
                     /site_type="active"
                     /db_xref="CDD:341237"
     Site            order(253,302..303,306,327,456..458,521,528)
                     /site_type="other"
                     /note="CoA binding site [chemical binding]"
                     /db_xref="CDD:341237"
     CDS             1..571
                     /gene="LOC144124624"
                     /coded_by="XM_077657426.1:1..1716"
                     /db_xref="GeneID:144124624"
ORIGIN      
        1 mtarsnspee pltsddqplh sevylviedn vvktcvknai vpdvnfvtff tnmwqkhedk
       61 valvdvksga qytfgellda srrvaaglrq lglrtgdvvg fhcangteli valcgtffag
      121 giaafvktnl tqgearhqlr dveptfvvcd iedikkikda cdglasvqll ivttgtcdea
      181 lslselkrsh peqiaaplnl nadsvlaily ssgstglpkg vqlnhrnlis qvishgyldp
      241 svfessdvyi avapimhvgg fwlifsylgq gckviavhtt dfdvvlpaie rhrptmtalf
      301 ptlllkldqh pllgkvdtss mkklfvggtt vsssllqsvv rklglkgvtq aygmteltgc
      361 itfstpldgd frsvgrpapf velkvadvct rkalgpnkqg eicvkgactf kaylrkpket
      421 ddiyedgfic tgdvgyyvpd grffvcgrlk elikcmdqqv apaeledlla adwgvrhvvv
      481 agvphaeyge tarafvvsrq rlrpgsieeq revqrlqelv aaelayhkhl hggvefldsi
      541 pltetgkyqr ralrdayiqr qglrtkepvl n
//
LOCUS       XP_077513582             502 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124647 [Amblyomma americanum].
ACCESSION   XP_077513582
VERSION     XP_077513582.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657456.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 30% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..502
                     /product="uncharacterized protein LOC144124647"
                     /calculated_mol_wt=56275
     CDS             1..502
                     /gene="LOC144124647"
                     /coded_by="XM_077657456.1:1..1509"
                     /db_xref="GeneID:144124647"
ORIGIN      
        1 marsstnaap dlppddeepf dvqaeqhdss ssdygyqlak llfkwkegrr lpestvrela
       61 ndiidfletv adhqqtepld gpaisvkeic klqldqlrtr sgrttywkah fpfvesrtvv
      121 lcgndtikyv plcdvlkcil krsehsnefs ndsrtdgymr svfdgcafqn hehfagntsk
      181 lclqlysyef eacnplgskr gkhkmtavyf svlnfdaklr salqginlvl lvkdkhiaty
      241 gfskilepfi tdvkiianhg itvnrnlvkg svfiftgdnl sshriggfkc tfsqgricry
      301 cmalrietdy khlqgdfvlr spqghkhhls mlraglptls lygvkepsal lcpgfdptqh
      361 lppdimhdlh egvlsfalrh iisalikerf fslsdlnksi sewlydphdi cnkpeaiakp
      421 ylqgkavlka satqskyssr sfcdgdevtf adevreqsga aeyceasdhk idlegtvgai
      481 cwtprgtrae gfeswsiqpm mg
//
LOCUS       XP_077513605             502 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124662 [Amblyomma americanum].
ACCESSION   XP_077513605
VERSION     XP_077513605.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657479.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 30% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..502
                     /product="uncharacterized protein LOC144124662"
                     /calculated_mol_wt=56209
     Region          156..>273
                     /region_name="Transposase_21"
                     /note="Transposase family tnp2; cl29371"
                     /db_xref="CDD:475188"
     CDS             1..502
                     /gene="LOC144124662"
                     /coded_by="XM_077657479.1:1..1509"
                     /db_xref="GeneID:144124662"
ORIGIN      
        1 maristnaap dlppddeepf dvqaeqhdss ssdygyqlak llfkwkegrr lpestvrela
       61 ndiidfletv adhqqtepld gpaisvkeic klqldqlrtr sgrttywkvh fpfvesrtvv
      121 lcgndtikyv plcdvlkcil krsehsnefs ndsrtdgymr svfdgcafkn hehfagntsk
      181 lclqlysdef evcnplgskr gkhkmtavyf svlnfdaklr salqginlvl lvkdkhiaty
      241 gfskilepfi tdvkiianhg itvnrnlvkg svfiftgdnl sshriggfkc tfsqgricry
      301 cmalrigtdy khlqgdfvlr spqghkhhls mlraglptls lcgvkepsal lcpgfdptqh
      361 lppnimhdlh egvlsfalrh iisalfeery fslsdlnksi sewlydphdi cnkpeaiakp
      421 ylqgkavlka satqskyssr sfcdgdevtf adevreqsga aeyceasdhk idlegtvgai
      481 cwtprgtrae gfeswsiqpm lg
//
LOCUS       XP_077513660             448 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124696 [Amblyomma americanum].
ACCESSION   XP_077513660
VERSION     XP_077513660.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657534.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..448
                     /product="uncharacterized protein LOC144124696"
                     /calculated_mol_wt=49928
     CDS             1..448
                     /gene="LOC144124696"
                     /coded_by="XM_077657534.1:12..1358"
                     /db_xref="GeneID:144124696"
ORIGIN      
        1 mdedsifwri frcflrseny fnrrgiydld ggdpgenara pepsneygrl irpnllrgpa
       61 ichrcflaeh lryynmvkrr mgrqeeaska egppaaaapa ngkrkttdes qstnvdnqek
      121 ykegqqeqqq qqhqrqqqhq qqqqqqqlkq qqqlkqqqlk qqqqqqqqqq qqqqqqqqqq
      181 qqqqqqqqqq qqqqqqhsta qqqqqqqqqq qqqqqqqqqq qqqqqqqqqq qqqqqqqywa
      241 ksldltsffa slniskifta tpkeadksag grgtdggtag gtpgqgsada hghsarhsrc
      301 gavtspcarg agcvakrgav gwnrrvrvlk levpvrlrrg tagpsgacap aeaaanianr
      361 eplrnsvved ggpasrlrsk tgvaktaaad stdrtavggr garrvdaqga adqpgegsat
      421 arggstrqrp strstrtaap rasgrrpr
//
LOCUS       XP_077513670             103 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124700 [Amblyomma americanum].
ACCESSION   XP_077513670
VERSION     XP_077513670.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657544.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 11% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..103
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..103
                     /product="uncharacterized protein LOC144124700"
                     /calculated_mol_wt=10085
     CDS             1..103
                     /gene="LOC144124700"
                     /coded_by="XM_077657544.1:56..367"
                     /db_xref="GeneID:144124700"
ORIGIN      
        1 miaavsgepv skgyvqsrss qslllfqeda dgasptgstl nqacshasgf imvqsdhtya
       61 gpsteasass lepgtagvaa tlsasadlsp galfssspls yeg
//
LOCUS       XP_077513753             263 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124760 [Amblyomma americanum].
ACCESSION   XP_077513753
VERSION     XP_077513753.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657627.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..263
                     /product="uncharacterized protein LOC144124760"
                     /calculated_mol_wt=27978
     CDS             1..263
                     /gene="LOC144124760"
                     /coded_by="XM_077657627.1:1..792"
                     /db_xref="GeneID:144124760"
ORIGIN      
        1 mtsslagify havidcarah vafsvlpdtl addppsccgt klvvaadtnv ppasavlvpv
       61 satsgssktv lfapspvlas rrhlplpfai vdirhgatel vvsnpppyqf tllrgeclgh
      121 veqldllmpf ddetlcggts vaaltspsav sdpfspemfh rsvasdldpa hhekllallh
      181 kfrssfdhqt tslgsattvv hcidtgphap lhqspyrvsp perrviaeqv ddmlhrgviq
      241 sssspwpspv vlvkegwfds vlc
//
LOCUS       XP_077513787             278 aa            linear   INV 25-NOV-2025
DEFINITION  cytoglobin-1-like [Amblyomma americanum].
ACCESSION   XP_077513787
VERSION     XP_077513787.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657661.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..278
                     /product="cytoglobin-1-like"
                     /calculated_mol_wt=31027
     Region          26..160
                     /region_name="Mb-like"
                     /note="myoglobin-like; M family globin domain; cd01040"
                     /db_xref="CDD:381254"
     Site            order(58..59,79,82..83,86,107,110..111,117,121..122,125)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:381254"
     CDS             1..278
                     /gene="LOC144124782"
                     /coded_by="XM_077657661.1:68..904"
                     /db_xref="GeneID:144124782"
ORIGIN      
        1 mgniqrktge lpdeltglte nevklvqqtw rkfssenpey gvllflamfl khpeyiqlfk
       61 pfrgksvavl kedptfrahg csigyhitsm veslhdpatf eilvrrnate hlrrkgvkpl
      121 hfevmgqciv dtlqatderh mtpaaveawg kfltcmvaii ndvydkaaae raekssssst
      181 saseyhsrtt tmldeamlts stkpvadgsr paadsrkrpp riirklsagp irdmsgteer
      241 gvdadavlmq ersaaydnkg trekaprkek gilrkhkp
//
LOCUS       XP_077513822             117 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124803 [Amblyomma americanum].
ACCESSION   XP_077513822
VERSION     XP_077513822.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657696.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 32% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..117
                     /product="uncharacterized protein LOC144124803"
                     /calculated_mol_wt=13354
     CDS             1..117
                     /gene="LOC144124803"
                     /coded_by="XM_077657696.1:70..423"
                     /db_xref="GeneID:144124803"
ORIGIN      
        1 melgvtkadl irrlarrkip vpdfdfeese pvatvsqena vsakdaannv ktakrqklqe
       61 lldgsssddd vlpkklleer rknaillrrl krvrkekael qarhdrvddh llnkfde
//
LOCUS       XP_077513835             461 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124809 [Amblyomma americanum].
ACCESSION   XP_077513835
VERSION     XP_077513835.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657709.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 27% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..461
                     /product="uncharacterized protein LOC144124809"
                     /calculated_mol_wt=51491
     CDS             1..461
                     /gene="LOC144124809"
                     /coded_by="XM_077657709.1:1..1386"
                     /db_xref="GeneID:144124809"
ORIGIN      
        1 mklvmlqkts vnglkgpspl wslkyldlvs cytveymhcv llgvtrqfte ywfdssscre
       61 kyyigrpstv nalnkrltsi rpphhvtrlp rtiqerhfwk ahewrnwllf ycvpcclntl
      121 paqflrhfal lceaifillq eqlssaainy adrllqrfvr raaalygerc msfnvhqlth
      181 ltqaarnfgp lwahsafgfe sgnghivklv taangvpdqi leraimsqel hlvlslnaip
      241 lrsqqlcsef lhypnvskah yvqgacmfgv pkevplllpa eraalelmln niprvqeyfr
      301 ffyrgtvmhs qqyqrgtksd ssviesqdge ffvikrifev vdggspsnge vvllcrkivc
      361 edcdlkkssc dggalasssq agavlrvspa dsgprpsism atngpprgpa freppskahc
      421 fnfvlknkas vdgivdgiea vvgpkglesl qhqgcfklsi a
//
LOCUS       XP_077513864             286 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124832 [Amblyomma americanum].
ACCESSION   XP_077513864
VERSION     XP_077513864.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657738.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 46% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..286
                     /product="uncharacterized protein LOC144124832"
                     /calculated_mol_wt=31581
     Region          1..>91
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     CDS             1..286
                     /gene="LOC144124832"
                     /coded_by="XM_077657738.1:1..861"
                     /db_xref="GeneID:144124832"
ORIGIN      
        1 mipkpgkmlq ienlrpislt scqgkpyeri vtkriqphle nkrlytdimf gvranlstqd
       61 lllqlkavle tmpkqeknvv maldikgafd nakadkqaig lahraveeei lfpksaldaq
      121 hmqdkvqlle akvksledkl rrqklemdsv vklnrqlqea mlikifraev vpvaeeanfd
      181 vlrdsqgnea llvdtapapa iasfhrsset smvpeqaval gtpahvdaat nkvschlpve
      241 tslvmneama wytpahalea cqshthdelp adssiklagc pcyscq
//
LOCUS       XP_077513903             456 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124859 [Amblyomma americanum].
ACCESSION   XP_077513903
VERSION     XP_077513903.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657777.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 33% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..456
                     /product="uncharacterized protein LOC144124859"
                     /calculated_mol_wt=48638
     Region          <243..>288
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     Region          282..>439
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..456
                     /gene="LOC144124859"
                     /coded_by="XM_077657777.1:1..1371"
                     /db_xref="GeneID:144124859"
ORIGIN      
        1 mtlspeenvw fesqflsesd pmksseakci llpaterdgr rimsshgaai aavagrgnrf
       61 asdadkdyei vlptlptgrv vlntvflhgd vrarpyrved frdalanaga lpdvvalgay
      121 qinhvwavtf stadaakkla atkelvvkgr rcivfdpedh qvklrlhwml hgvagedirt
      181 afaafgnvte vtrerwrvag mkekgsttrt vllklkpgvk vddlphqvrv agelalvvvp
      241 grpmqrlrcg gtghvrrdck vprcskcrry ghseadcvrn yasatghvaa svsaelmdvv
      301 eaeeaasgad dtgkaegvav qpavassntd tavaqesgsp ttdpsstpve tpkdgasssv
      361 hverkaases hdekdesrmn vgvttpakrp haetkadgek aaepsvgepp aktpqarrag
      421 lrprpkvpae krggdkappd qehvlapddt ggdgiv
//
LOCUS       XP_077513916             564 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124866 [Amblyomma americanum].
ACCESSION   XP_077513916
VERSION     XP_077513916.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657790.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..564
                     /product="uncharacterized protein LOC144124866"
                     /calculated_mol_wt=62336
     Region          330..505
                     /region_name="RT_LTR"
                     /note="Reverse transcriptases (RTs) from retrotransposons
                     and retroviruses which have long terminal repeats (LTRs)
                     in their DNA copies but not in their RNA template. RT
                     catalyzes DNA replication from an RNA template, and is
                     responsible for the replication of...; cd01647"
                     /db_xref="CDD:238825"
     Site            order(394..399,428..429,454,456..457,501..502)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238825"
     Site            order(394..399,428,456)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238825"
     Site            429
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238825"
     CDS             1..564
                     /gene="LOC144124866"
                     /coded_by="XM_077657790.1:1..1695"
                     /db_xref="GeneID:144124866"
ORIGIN      
        1 mnqpsdagsp tvavldvklp pfwtgdpelw fvqvesqfaa rritadstky hhvvgslpha
       61 tasevrdlll appaenayqt lketlirrvt pteperlqqp lreaelgdrr psqllchmqq
      121 laggttasdg rlvrelflqr lptsvriglt asgdtdlakm aeladklmav avptvasvha
      181 dappanslqa mreeisrlad tvaalhssgn qrprqrtasp qqrrvcwyhr kfgnaarncp
      241 lshaevrgkp srhpplsptl vappgatrft gilgefpelt rppqasaavk hnvchhivtt
      301 gppvyarprr lspqklqaar qefdhmlelg iirpsaspwa splhmvpkst lgdwrpcgdy
      361 ralnratvpd rypvphihdv tsslhgvaif skidlvrayh qipvapedvs ktaittpfgl
      421 feflrmpfgl rnsgqtfqrf ingvlhgldf chaylddlli astssdehea hlrsvfqrla
      481 ehgilvntdk celgaekltf lghvvsssgi qphpdkvkav ekfprpttkr qlreflglvl
      541 yrersiplhr plsvasyval irsf
//
LOCUS       XP_077513952             357 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124886 [Amblyomma americanum].
ACCESSION   XP_077513952
VERSION     XP_077513952.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657826.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..357
                     /product="uncharacterized protein LOC144124886"
                     /calculated_mol_wt=40812
     Region          <14..47
                     /region_name="bZIP"
                     /note="Basic leucine zipper (bZIP) domain of bZIP
                     transcription factors: a DNA-binding and dimerization
                     domain; cl21462"
                     /db_xref="CDD:473870"
     Site            order(14..15,18..19,21..22,25..26,28..29,32..33,35..36,
                     39..40,42..43)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269834"
     Region          110..158
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          189..348
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..357
                     /gene="LOC144124886"
                     /coded_by="XM_077657826.1:1..1074"
                     /db_xref="GeneID:144124886"
ORIGIN      
        1 mgppdaasaa tlrkvahlhe hceklehens lfrkdkselq kevedlkpql hdvkvealke
       61 klkvhdfvsd ekrlvfytgf nsfkkfdafv ehvecmyqal ktgagrppal tmteqliavl
      121 crlrvglleq dlayrlkvsv atvsamysfw vnflyemliq vpmwpsrntv dlfmpdkfke
      181 lypstrivld cteifienps dyktqsdtfs sykghntakg ligitpngsv sfvsnlapgr
      241 lsdreitrhs gmyelleegd svmadigfli eddlrqlgvk lnmppmlngr tqlsladela
      301 trqiaslrih vervirevkn frilravfpn vmaeklnkiw kiccylnnfm hkpilnr
//
LOCUS       XP_077513979             116 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124903 [Amblyomma americanum].
ACCESSION   XP_077513979
VERSION     XP_077513979.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657853.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 41% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..116
                     /product="uncharacterized protein LOC144124903"
                     /calculated_mol_wt=13083
     CDS             1..116
                     /gene="LOC144124903"
                     /coded_by="XM_077657853.1:1..351"
                     /db_xref="GeneID:144124903"
ORIGIN      
        1 mehnawqmll rtptdpmffr satqvfwmpd elksrsvtgm lsnkcrslgr teakpaltpe
       61 kvsslkgasa ffllplfhtn mgdvptelae krlkavrkhl sqklgdmlrr trsppe
//
LOCUS       XP_077514000             138 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124922 [Amblyomma americanum].
ACCESSION   XP_077514000
VERSION     XP_077514000.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657874.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 14% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..138
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..138
                     /product="uncharacterized protein LOC144124922"
                     /calculated_mol_wt=15343
     Region          64..>122
                     /region_name="BEN"
                     /note="BEN domain; pfam10523"
                     /db_xref="CDD:463133"
     CDS             1..138
                     /gene="LOC144124922"
                     /coded_by="XM_077657874.1:78..494"
                     /db_xref="GeneID:144124922"
ORIGIN      
        1 msflflllav pakmvkrler mleskkepdq pvvpcakvdi gngvlvyrti lqmlerqcqs
       61 spgkfarall rnvfteeelr gkslygmknn vqkglpvkea ldpvrlsavi gytcdhfpvv
      121 nvaylknsla allarelk
//
LOCUS       XP_077514014             129 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124929 [Amblyomma americanum].
ACCESSION   XP_077514014
VERSION     XP_077514014.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657888.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..129
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..129
                     /product="uncharacterized protein LOC144124929"
                     /calculated_mol_wt=14031
     Region          <12..38
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Region          99..>129
                     /region_name="RT_RNaseH_2"
                     /note="RNase H-like domain found in reverse transcriptase;
                     pfam17919"
                     /db_xref="CDD:465567"
     CDS             1..129
                     /gene="LOC144124929"
                     /coded_by="XM_077657888.1:1..390"
                     /db_xref="GeneID:144124929"
ORIGIN      
        1 mapprapgas lfrhaglpln sskcrfarlq irilghvvda tgvhpdpaki ravkdfpepr
       61 fcndvrsfsg lcsyfrrfvp adiarplsdl lkkdmtvtwg plqenpfgal isklitspvl
      121 ahfdvaapt
//
LOCUS       XP_077514045             172 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124952 [Amblyomma americanum].
ACCESSION   XP_077514045
VERSION     XP_077514045.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657919.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 39% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..172
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..172
                     /product="uncharacterized protein LOC144124952"
                     /calculated_mol_wt=18499
     Region          5..>53
                     /region_name="GT1"
                     /note="myb-like, SANT family; cl23759"
                     /db_xref="CDD:474047"
     CDS             1..172
                     /gene="LOC144124952"
                     /coded_by="XM_077657919.1:1..519"
                     /db_xref="GeneID:144124952"
ORIGIN      
        1 magerkkinf tederrilid lvdrrksvie nkrtdavsvn ekkvgleieq qfnpilnpss
       61 ftwgggsavp sslteelegv gavashmsev idnpfdgdrp rqeganaasa decraavagl
      121 lapmqtqait aasvalngne gvdellqfiy egdtevmppe styqlppppp rh
//
LOCUS       XP_077514060             132 aa            linear   INV 25-NOV-2025
DEFINITION  high-affinity choline transporter 1-like [Amblyomma americanum].
ACCESSION   XP_077514060
VERSION     XP_077514060.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657934.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 16% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..132
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..132
                     /product="high-affinity choline transporter 1-like"
                     /calculated_mol_wt=14459
     Region          11..>111
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     CDS             1..132
                     /gene="LOC144124955"
                     /coded_by="XM_077657934.1:28..426"
                     /db_xref="GeneID:144124955"
ORIGIN      
        1 msvsilgalv milyyiavvs vgvwsgrkvh gdlshnyssg rrkgeykpdn deyllrlfla
       61 nrnvplalgi ismtatwvcg vfingtaeav frrgiiwcqa pfghalslmv gewtrplvlp
      121 raacprssyc tl
//
LOCUS       XP_077514072             160 aa            linear   INV 25-NOV-2025
DEFINITION  high-affinity choline transporter 1-like [Amblyomma americanum].
ACCESSION   XP_077514072
VERSION     XP_077514072.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657946.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..160
                     /product="high-affinity choline transporter 1-like"
                     /calculated_mol_wt=17442
     Region          39..>139
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     CDS             1..160
                     /gene="LOC144124956"
                     /coded_by="XM_077657946.1:271..753"
                     /db_xref="GeneID:144124956"
ORIGIN      
        1 masrgagara iyeekpgvns eqrqrgeams vsilgalvmi lyyiavvsvg vwsgrkvhgd
       61 lshnyssgrr kgeykpdnde yllrlflanr nvplalgiis mtatwvggvf ingtaeavfr
      121 rgiiwcqapf ghalslmvge wtrplvlpra vcprssyctl
//
LOCUS       XP_077514084             555 aa            linear   INV 25-NOV-2025
DEFINITION  high-affinity choline transporter 1-like [Amblyomma americanum].
ACCESSION   XP_077514084
VERSION     XP_077514084.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657958.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..555
                     /product="high-affinity choline transporter 1-like"
                     /calculated_mol_wt=58374
     Region          1..379
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     Region          <452..>542
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     CDS             1..555
                     /gene="LOC144124963"
                     /coded_by="XM_077657958.1:19..1686"
                     /db_xref="GeneID:144124963"
ORIGIN      
        1 mrvtkaltml dpfqqhygnw isiiltvpav cgevfwtasi lsalgdtvht mvhvnselii
       61 ivsavvilfy tslgglfsvi ytdlfqmgst mvglwscvpy vmmskasgki gdsdwvgeie
      121 pkeasqvidq lfmtvfggip wqvyfqrvls tdsifsakml syvscmgcil vavpsvivga
      181 qakttnftal gyigphnlte ehrkdvlpqa irymtpslva ilgqlgitaa vmssmdssml
      241 sasslitrnv yhflirpsas dgeislvlrs miclvgatat vtalsvesvf glwalssdlv
      301 yvllfpqfva lfymrkksna ygavfgfllg lilrvlcgep vmdvpavikl pmyddergqq
      361 fpfrtmcmlv sligvllgss iagylfraai lpprldvcrs faelhprpes ptsaaaeaat
      421 asdaapvast taeasdgapg aaattatvea asgaaapgvs aasnvtsgaa tsvtspavav
      481 qaagqpqphp qtqqphaqpq prpqeappkq pqeappglsa vasptsadvv asgsalavpa
      541 apkkvkgqks kkkrk
//
LOCUS       XP_077514106             281 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144124980 [Amblyomma americanum].
ACCESSION   XP_077514106
VERSION     XP_077514106.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077657980.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..281
                     /product="uncharacterized protein LOC144124980"
                     /calculated_mol_wt=31825
     Region          180..259
                     /region_name="GIY-YIG_SF"
                     /note="GIY-YIG nuclease domain superfamily; cl15257"
                     /db_xref="CDD:472790"
     Site            order(180..182,192..194)
                     /site_type="other"
                     /note="GIY-YIG motif/motif A"
                     /db_xref="CDD:198380"
     Site            order(182,192,194,203,207,251)
                     /site_type="active"
                     /db_xref="CDD:198380"
     Site            order(203,251)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:198380"
     Site            251
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:198380"
     CDS             1..281
                     /gene="LOC144124980"
                     /coded_by="XM_077657980.1:1..846"
                     /db_xref="GeneID:144124980"
ORIGIN      
        1 mptnneirfl dirfkflenh vcwsyspran kplipytsah sklvkrgiin lclnnaleks
       61 cphliseslh eqiarfheag ypadicvsva erilkkkrqe sllpsgpser kekiavipym
      121 hclshdlkki gtkanvrvvf sapdklials kktlpaderp qkmqctikhr ekfvkctegi
      181 vydiplscgk ryvgqsgrcl nerlrehnnn lrtltgsnla lhckecgcvp yldkcsifar
      241 hrdqltrevi eaariaalqd kcvskpsisl skrelaflki n
//
LOCUS       XP_077514155             225 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125005 [Amblyomma americanum].
ACCESSION   XP_077514155
VERSION     XP_077514155.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658029.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..225
                     /product="uncharacterized protein LOC144125005"
                     /calculated_mol_wt=25000
     CDS             1..225
                     /gene="LOC144125005"
                     /coded_by="XM_077658029.1:1..678"
                     /db_xref="GeneID:144125005"
ORIGIN      
        1 mptygalapf hgeggawtey lervklflda nsvpeeekrf vfltccgsst ysllrslltp
       61 ktpdqvside ifsvlsghyi qnpsvvvccf rfssrprqpe esvsdytaal kklsancecg
      121 tflpdmlrdr lvggindasm qrrlleepnl tfesaveili ametakrdss mlmqaqgmte
      181 aqtvhstvki mnlsftvivg yippcvnfdv drlqdivrst lrhts
//
LOCUS       XP_077514166             186 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125015 [Amblyomma americanum].
ACCESSION   XP_077514166
VERSION     XP_077514166.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658040.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 24% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..186
                     /product="uncharacterized protein LOC144125015"
                     /calculated_mol_wt=21864
     CDS             1..186
                     /gene="LOC144125015"
                     /coded_by="XM_077658040.1:105..665"
                     /db_xref="GeneID:144125015"
ORIGIN      
        1 mdretiarll tiripslaem eakrkaanes ydsdvppeeq rhmsdqrrrl lnirpagedt
       61 ihwcwlcdrr fhlpsrliah qiyqhdfdge prpikvpcpi cyrlcrnefi mglhlgthig
      121 errcfkcgae fasatsahah kllhhnrgnf qcrfcrklfa lkskrddhcr lehlrqlkrq
      181 rsatsf
//
LOCUS       XP_077514179             205 aa            linear   INV 25-NOV-2025
DEFINITION  mRNA-capping enzyme-like [Amblyomma americanum].
ACCESSION   XP_077514179
VERSION     XP_077514179.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658053.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 29% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..205
                     /product="mRNA-capping enzyme-like"
                     /calculated_mol_wt=23291
     Region          <64..80
                     /region_name="Adenylation_DNA_ligase_like"
                     /note="Adenylation domain of proteins similar to
                     ATP-dependent polynucleotide ligases; cl12015"
                     /db_xref="CDD:448381"
     Region          84..178
                     /region_name="mRNA_cap_C"
                     /note="mRNA capping enzyme, C-terminal domain; pfam03919"
                     /db_xref="CDD:461093"
     CDS             1..205
                     /gene="LOC144125022"
                     /coded_by="XM_077658053.1:1..618"
                     /db_xref="GeneID:144125022"
ORIGIN      
        1 mkariedgiv lspypsieip mcsfynlare klltnpgkta lllsdkfapk hatragwthl
       61 patmdpyvfg rckevlkwkp pqlntidfrl kitresgmgl vpksigllfv ggkeqpysri
      121 tkvtkqlkal dnkiiecrve dgqwvllrer tdksfpnsfs taegvmesir npidkeylld
      181 fiskhlwrkk ptdselmppp nrvah
//
LOCUS       XP_077514193             474 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125029 [Amblyomma americanum].
ACCESSION   XP_077514193
VERSION     XP_077514193.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658067.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..474
                     /product="uncharacterized protein LOC144125029"
                     /calculated_mol_wt=52389
     CDS             1..474
                     /gene="LOC144125029"
                     /coded_by="XM_077658067.1:1..1425"
                     /db_xref="GeneID:144125029"
ORIGIN      
        1 mpyqrcadtw rivgneasai gltlsfflss krtwlhyvkf gtaifigtfl vagglfviir
       61 qvtgrhatrp ragtykpvvs vgakvhhhaf ndddddappq lsrmpfactv glsldllkha
      121 nlllredglc eyvifqstfv adgdgkvdlw dghnhtrsfr tflefarvar ksaygvaith
      181 rksrealrql gsaagigefv sywskgvrhh avldvdersv sgprdlrstf allkafrllq
      241 qaqtdvnnss gayiffgctt avpknsllhd ilkvelrslp vdvmilttha mvsedsekvv
      301 vppttlrale gshlpaildv ahhaageeyl hrlsavaltl pvsvlayevp ertregaacd
      361 sgaievsvke vcsksnypld vkygnntiig rfysptmgrl ylfdtketfh fkmctlretf
      421 pelvhgwvvf gvdladydgv cngasrtldr vrairktfde ylkalpgqpp deac
//
LOCUS       XP_077514218             207 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125043 [Amblyomma americanum].
ACCESSION   XP_077514218
VERSION     XP_077514218.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658092.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..207
                     /product="uncharacterized protein LOC144125043"
                     /calculated_mol_wt=23641
     CDS             1..207
                     /gene="LOC144125043"
                     /coded_by="XM_077658092.1:1..624"
                     /db_xref="GeneID:144125043"
ORIGIN      
        1 mssklesles svsllsdkfd efqtrllaqe kstkdvtkrv erlekaewac eiaqlnkdvd
       61 slewrsrrln ieihglpete nedlltkvne igtkleldev cssditalhr lpakpgkirg
      121 vivrfakqev rdawlakrqa lrddntgkym cenmtrytrt lltvakewak esgyafvwhv
      181 ngkvlvrkqa garavvirdk ddlanlr
//
LOCUS       XP_077514242             364 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125056 [Amblyomma americanum].
ACCESSION   XP_077514242
VERSION     XP_077514242.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658116.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..364
                     /product="uncharacterized protein LOC144125056"
                     /calculated_mol_wt=41540
     Region          5..80
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          153..362
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..364
                     /gene="LOC144125056"
                     /coded_by="XM_077658116.1:56..1150"
                     /db_xref="GeneID:144125056"
ORIGIN      
        1 mvascvaygc anrlkkgcgl tfhlfpkype lrslweravr rerwhakdgd rlccvhfape
       61 cfdrtgqttr lragsvpsvf pafpehlqkp vkrkrpgrkh edipanscsp qhekseaeee
      121 ppvpsptkqw ykekvsasee eithlkkkvk tlqqtkrrlt krnetaqeii keirdqklls
      181 eegsqvftss issdiqqlln ragnnqkgvy tpelrafalt lhfyspdayd yvrskfndal
      241 psqwtlrqwy rsvhgapgft deafcflqkf tqsrnepfyc aliaddmair khvelvgnki
      301 vgyvnfgtgl dddslpearn vcvymivgvn vrikmpvayf lidtltgaer aeltkqcihk
      361 ldls
//
LOCUS       XP_077514266             122 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125071 [Amblyomma americanum].
ACCESSION   XP_077514266
VERSION     XP_077514266.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658140.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 30% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..122
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..122
                     /product="uncharacterized protein LOC144125071"
                     /calculated_mol_wt=12951
     CDS             1..122
                     /gene="LOC144125071"
                     /coded_by="XM_077658140.1:1..369"
                     /db_xref="GeneID:144125071"
ORIGIN      
        1 mphhckkahm kataavalea pkaaafeeea asserkaead eeevvgaasl essphseril
       61 hegevaaaqy eevskelhgr gcltvqiwgc sslcpfsmhc saqalhaasf rvpvqatsri
      121 aw
//
LOCUS       XP_077514302             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125089 [Amblyomma americanum].
ACCESSION   XP_077514302
VERSION     XP_077514302.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658176.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 37% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144125089"
                     /calculated_mol_wt=23794
     CDS             1..217
                     /gene="LOC144125089"
                     /coded_by="XM_077658176.1:207..860"
                     /db_xref="GeneID:144125089"
ORIGIN      
        1 mnrmaaavpa ilrarnssqa rdpthrppfa altsvlccqq pasigpcvlp afscvippdp
       61 fmlktkscme avqatklrvi fardleqagk lvdgqwysls kasffsktkt iflgswamyl
      121 etcgqdnagq hasasskkkh etdktwdviv pcvvfaynta lqeatqmtpf rlahgrkstt
      181 tfdamlqtvt deenfnvaay lqradearla glrikdl
//
LOCUS       XP_077514315             229 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125097 [Amblyomma americanum].
ACCESSION   XP_077514315
VERSION     XP_077514315.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658189.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 48% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..229
                     /product="uncharacterized protein LOC144125097"
                     /calculated_mol_wt=25661
     Region          <62..>170
                     /region_name="PspC_subgroup_1"
                     /note="pneumococcal surface protein PspC, choline-binding
                     form; NF033838"
                     /db_xref="CDD:468201"
     CDS             1..229
                     /gene="LOC144125097"
                     /coded_by="XM_077658189.1:59..748"
                     /db_xref="GeneID:144125097"
ORIGIN      
        1 myvlvryage drsyilqhtd vrdfvphdie dydpkkvydi fwagdsstcg gyydgkiihm
       61 tdteeemeqw rkrkearkca rktdipprkk arleeqakgv akknaerell ssrvgnspek
      121 skvahleslv rdlkaelerc kevigdkeke mknlrslnes lqkalscrsa mplatsrgtt
      181 aveqgvgcpc aalarasass fpgtptwlgi qareieqpwe qilsslaas
//
LOCUS       XP_077514328             232 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125105 [Amblyomma americanum].
ACCESSION   XP_077514328
VERSION     XP_077514328.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658202.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..232
                     /product="uncharacterized protein LOC144125105"
                     /calculated_mol_wt=26118
     CDS             1..232
                     /gene="LOC144125105"
                     /coded_by="XM_077658202.1:1..699"
                     /db_xref="GeneID:144125105"
ORIGIN      
        1 mrdvyeqgrp vhgvkgpssl nqlqgfnlvw clppdfmhcv legvtqqite lwlgatgkaf
       61 higrnfhele rrikvlrppi sfcrlprpis erknwkatew lfwllyyslp cvsglipsry
      121 vshfsllcqg vflllqtsvs dadlqeadql lscfvnrics lygessatfn vhqllhlaks
      181 vemlgplwgt stfpfengng qllklvtaaq gvplqiaerc itkswlktsk vc
//
LOCUS       XP_077514340             298 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125114 [Amblyomma americanum].
ACCESSION   XP_077514340
VERSION     XP_077514340.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658214.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..298
                     /product="uncharacterized protein LOC144125114"
                     /calculated_mol_wt=32924
     Region          <62..>169
                     /region_name="PspC_subgroup_1"
                     /note="pneumococcal surface protein PspC, choline-binding
                     form; NF033838"
                     /db_xref="CDD:468201"
     CDS             1..298
                     /gene="LOC144125114"
                     /coded_by="XM_077658214.1:41..937"
                     /db_xref="GeneID:144125114"
ORIGIN      
        1 myvlvryage drsyilqhtd vrdfvphdie dydpkkvydi fwagdsstcg gyydgkiihm
       61 tdteeemkqw rkrkearkca rktdipprkk arleeqakgv akknaerell ssrvgnspee
      121 skvahleslv rdlkaelerc kemigdkeke mktlrslnes lqkalcanil qndhsqnenv
      181 saaidkvfwe gdsatqavns amhshslgti apvlpldvas plaappvapp radvapprad
      241 vappradvap pradvtpavp parpmskapr kaaqdldltv gttnetgeal ccwplrra
//
LOCUS       XP_077514353             349 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125119 [Amblyomma americanum].
ACCESSION   XP_077514353
VERSION     XP_077514353.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658227.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..349
                     /product="uncharacterized protein LOC144125119"
                     /calculated_mol_wt=39557
     CDS             1..349
                     /gene="LOC144125119"
                     /coded_by="XM_077658227.1:109..1158"
                     /db_xref="GeneID:144125119"
ORIGIN      
        1 mtskclvtfr ernfvlpfdg tltrrglver vgdhelfrgi dvatliftmf eedfnvfvdi
       61 pedfeirdra kikvsedcqv riadvldepq qaeqsrvtat ysltyslpav pndikfsier
      121 hqsgqyfrar srvvqwlyhd lclytmypgk lyneaaqalv srypnladss gtgyaqlefe
      181 litsvpvler leealqrtke knvhaarkkr hlegfledfd armdgtseaa vyettvtaav
      241 cllpsmvker vetfvrpfdp aavhyiptvv hnggiltttd fsvclekici ketsllaava
      301 tqmalywafn ivfdkkaqrs fdllcrlinv dsglrptplv rlaqtvmgk
//
LOCUS       XP_077514461             113 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125176 [Amblyomma americanum].
ACCESSION   XP_077514461
VERSION     XP_077514461.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658335.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..113
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..113
                     /product="uncharacterized protein LOC144125176"
                     /calculated_mol_wt=12202
     Region          49..>113
                     /region_name="RT_RNaseH_2"
                     /note="RNase H-like domain found in reverse transcriptase;
                     pfam17919"
                     /db_xref="CDD:465567"
     CDS             1..113
                     /gene="LOC144125176"
                     /coded_by="XM_077658335.1:1..342"
                     /db_xref="GeneID:144125176"
ORIGIN      
        1 mkefrvprsg ndvqsflalc syirpfvpgy aaiarplcdr lkkdmastwg plqenafaav
       61 isklttspvl ahfdvvvpte vrtdasahgi gpvlsrrqsg sdrviayasr pte
//
LOCUS       XP_077514474             183 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125180 [Amblyomma americanum].
ACCESSION   XP_077514474
VERSION     XP_077514474.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658348.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="uncharacterized protein LOC144125180"
                     /calculated_mol_wt=21248
     CDS             1..183
                     /gene="LOC144125180"
                     /coded_by="XM_077658348.1:1..552"
                     /db_xref="GeneID:144125180"
ORIGIN      
        1 mtfecfcnmd glshdaqlrs rrtrneeerr hherdlarca srrlrpctpa rlstglgkkk
       61 qgaaerrrlq rrrleilssr rppyflvpcs hprafppldd yrslcehhls fyerirgaqi
      121 pgsvavvnqq pprnqelrea eretslqsrl qsalsdavqt lshllnfaig ralhavrrif
      181 lyr
//
LOCUS       XP_077514487             183 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125186 [Amblyomma americanum].
ACCESSION   XP_077514487
VERSION     XP_077514487.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658361.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="uncharacterized protein LOC144125186"
                     /calculated_mol_wt=21262
     CDS             1..183
                     /gene="LOC144125186"
                     /coded_by="XM_077658361.1:1..552"
                     /db_xref="GeneID:144125186"
ORIGIN      
        1 mtfecfcnmd glshdaqlrs rrtrneeerr hherdlarca srrlrpctpa rlstglgkkk
       61 qgaaerrrlq rrrleilssr rppyflvpcs hprafppldd yrslcehhls fyerirgaqi
      121 pgsvavvnqq pprnqelrea eretslqsri qsalsdalqt lshllnfaig ralhavrrif
      181 lyr
//
LOCUS       XP_077514503             257 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125187 [Amblyomma americanum].
ACCESSION   XP_077514503
VERSION     XP_077514503.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658377.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 34% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..257
                     /product="uncharacterized protein LOC144125187"
                     /calculated_mol_wt=29223
     CDS             1..257
                     /gene="LOC144125187"
                     /coded_by="XM_077658377.1:1..774"
                     /db_xref="GeneID:144125187"
ORIGIN      
        1 mtfecfcnmd glshdaqlrs rrtrneeerr hherdlarca srrlrpctpa rpstglgkkk
       61 qgaaerrrlq rrrleilssr rppyflvpcs hprafppldd yrslcehhls fyerirgaqi
      121 pgsvavvnqq pprnqelrev aretslqsri qsalsdalqt lshllnfaig ralhasekrl
      181 lqsctdgckq rlffptfmkk ptpqsmrtdt sniglgdvys snskvawkvs srmqaagpla
      241 lqqtekecla lvwaamk
//
LOCUS       XP_077514518             183 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125188 [Amblyomma americanum].
ACCESSION   XP_077514518
VERSION     XP_077514518.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658392.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="uncharacterized protein LOC144125188"
                     /calculated_mol_wt=21216
     CDS             1..183
                     /gene="LOC144125188"
                     /coded_by="XM_077658392.1:1..552"
                     /db_xref="GeneID:144125188"
ORIGIN      
        1 mtfecfcnmd glshdaqlrs rrtrneeerr hherdlarca srrlrpctpa rpstglgkkk
       61 qgaaerrrlq rrrleilssr rppyflvpcs hprafppldd yrslcehhls fyerirgaqi
      121 pgsvavvnqq pprnqelrev aretslqsri qsalsdalqt lshllnfaig ralhavrrif
      181 lyr
//
LOCUS       XP_077514548             169 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125209 [Amblyomma americanum].
ACCESSION   XP_077514548
VERSION     XP_077514548.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658422.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 16% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..169
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..169
                     /product="uncharacterized protein LOC144125209"
                     /calculated_mol_wt=17178
     CDS             1..169
                     /gene="LOC144125209"
                     /coded_by="XM_077658422.1:1..510"
                     /db_xref="GeneID:144125209"
ORIGIN      
        1 mlgqpkqksk evdarkkrdr hktgggsaqc tvsaqseqvi avashimtrv gnqtdsdggi
       61 dlppvaslpv irllqpmvdg agneefhyae ddwepplvde pqspaaavgk sgatpeaieq
      121 esnlffpavq padasstaaa gcsnatvasg aaagpttaea vatgnpfqt
//
LOCUS       XP_077514559            1363 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125219 [Amblyomma americanum].
ACCESSION   XP_077514559
VERSION     XP_077514559.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658433.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1363
                     /product="uncharacterized protein LOC144125219"
                     /calculated_mol_wt=153216
     Region          79..216
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     Region          489..708
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(551..556,597..598,633,635..636,704..705)
                     /site_type="active"
                     /db_xref="CDD:238822"
     Site            order(551..556,597,635)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238822"
     Site            598
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238822"
     Region          725..887
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          <856..939
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     Region          1108..1167
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     CDS             1..1363
                     /gene="LOC144125219"
                     /coded_by="XM_077658433.1:1..4092"
                     /db_xref="GeneID:144125219"
ORIGIN      
        1 mvpeedaene yqqvydyqdr iasclaglql rvdsgssttq aavnsagsgt aiqtttpapi
       61 tttsvktrvk lpkielirft grrsdwqafw evfeqvvdkn edltdtdrfh ylrasvtgda
      121 aaalaglpst srcyndaiql lrkrfgneel lvqeylksli dlkpvrssed vrglrrlydt
      181 laahirglea lgrkldtfss mllpiaqram prdilldfnr rcvvdtdrla seqqgsvsda
      241 sphttdetgk ghqeerefri vrvtlktrrg cerelevlet dvicdqpips tprnvlqkli
      301 slgyeaadfs tdsgaekvel ligsdhlwdf ttgrcvklgk rlravetsig wtvqgpiesd
      361 ieqtnclhtv tlrtsvieke ttdiltkvwt lesigvnese dnekpceale ffednikrdg
      421 nryevalpwk seislednte ialkrlnqla nrlrrapdll qeydttirqy gvsdiaerve
      481 detsrnhciy hmahqavird srklrvvfda ssreknsksl ndnletgpnl tadlvgllln
      541 frkyrvalva diqqaflqig irepdrdafr ffwfretpqp gsplppietw rmkrvsfgtt
      601 assfllsatl qhhlkscveg fphtasqlqq glyvdelltg addeeeavkl yteanailha
      661 asmmlhkwss nnerlrqlfg qdcnetkplg fvsgvlkvlg lawdpttdrl tfascvtddt
      721 gkpdtkrsml qttariydpl gwlspiivra kilfqnlwrr nvewddplps dvaeswltwh
      781 gelkhlsevq vplyygadvs rqatktdlhi fadaspvayg avayltiase dglnstvvms
      841 ksrvaplkel ilarlelmac llaarlcsyi lstiglhpgr vilwtdsaia lhwirgdarr
      901 wqqfvrnrvc eiqhvtggye wrhcpgkknp adlltrgvsv shlaasdvww tgpqwlrkpd
      961 gewpqglvqn afsdaelelk qptkalpams sqvpppslmd iqryssfirl irvtawvlrf
     1021 vsrmrrrnde vgaltaeeln qaeifwirne qregfhrelk svhnqqafpv dsplrnaslm
     1081 vddkgilrid grlqrshldy dakhpvvipk qshlskllal ychrkvlhgg irctltqlre
     1141 rywvigarql vkrtlkecvt cqrfnaqpan qvtaplpkdr iteaqpfqvt gvdfagplvv
     1201 kgtsptkcyi alftcavtra ihlelvsdms agsfllafrr ftarrglpst iysdnaltfk
     1261 kvnkdlgrlw dvmrsgqvqe yigtlslkem vhdalipkls sapwryvlng kvetmngggg
     1321 sngqaaadgl paclpvgglr dfpiqtslka seikvslpfa asp
//
LOCUS       XP_077514572             306 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125227 [Amblyomma americanum].
ACCESSION   XP_077514572
VERSION     XP_077514572.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658446.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 25% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..306
                     /product="uncharacterized protein LOC144125227"
                     /calculated_mol_wt=34796
     Region          <1..44
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     CDS             1..306
                     /gene="LOC144125227"
                     /coded_by="XM_077658446.1:114..1034"
                     /db_xref="GeneID:144125227"
ORIGIN      
        1 meinvsktkt mtftrasnvh lssyfmnsic ienvytlkyl gvhltsnltw ndhideiisk
       61 anktlafirr nlylanqstk lltytalvrs kieyasiiwn pnqtylinkl eslqnkaarf
      121 itktysrass itaikkslql psletrrlla llshfhrlyh apssftashi kppqkifprl
      181 dhpfkvrpmf artnllrqsp lflaihhwtn lpkqiasird hdsfvsnlkr sfthvglpqd
      241 vqkvlalgpk fvgqekrsap elvsfvgrva rcasaeeplr cvsdamdvsh rckqpvpkvq
      301 edvkcg
//
LOCUS       XP_077514595             111 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125243 [Amblyomma americanum].
ACCESSION   XP_077514595
VERSION     XP_077514595.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658469.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..111
                     /product="uncharacterized protein LOC144125243"
                     /calculated_mol_wt=12728
     CDS             1..111
                     /gene="LOC144125243"
                     /coded_by="XM_077658469.1:1..336"
                     /db_xref="GeneID:144125243"
ORIGIN      
        1 meralqvttr gddldlllrv pleehmtand aqlsqfvmnt wvafirrgse tptmnmrrpw
       61 pqfrkgaevk vtvasdglrl sstwrsdife klylilfrlq kgssvkplys i
//
LOCUS       XP_077514606             541 aa            linear   INV 25-NOV-2025
DEFINITION  acetylcholinesterase-like [Amblyomma americanum].
ACCESSION   XP_077514606
VERSION     XP_077514606.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658480.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..541
                     /product="acetylcholinesterase-like"
                     /calculated_mol_wt=59409
     Region          11..493
                     /region_name="Abhydrolase"
                     /note="alpha/beta hydrolases; cl21494"
                     /db_xref="CDD:473884"
     Site            order(122..124,203..205,208,352,379,441,444)
                     /site_type="other"
                     /note="substrate binding pocket [chemical binding]"
                     /db_xref="CDD:238191"
     Site            order(204,327,440)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238191"
     CDS             1..541
                     /gene="LOC144125253"
                     /coded_by="XM_077658480.1:133..1758"
                     /db_xref="GeneID:144125253"
ORIGIN      
        1 mgvrssnrpv vvetregdve gimmitptqe rgsvfaflgv pygrdtsgpr rfappepalr
       61 rphllaanhr rpacpqfaqv pvldeipagt sedclhlnvw tptiapcatg cqlkpvlvff
      121 ygrdfehgfs dyqyfdgasl sgytdtvvvv psyrlglfgf lngntndtpg nvglldqlla
      181 lnwiknniaa fhgdprnval vgydtgassl gylmmadgsa pfsrfffhsg splrllpens
      241 gaaglanmra vadlvscsha sdertvaclr rasindlvaa aqklslalgp rfgpsigvpp
      301 llgppqlqea avyaadrevi ightadegrf qlyqleqtvl kdevgnttrl amgleqvire
      361 flhnvniswq qrmqldelat dinraglpsl sgwrnfvgsl lfscpvqyft etlaqssrat
      421 vyafefshrr lghrvqvttr gddldllfgv pleehmtand aqlskfvmnt wvafirrgse
      481 tptmnmrrpw pqfrkgaevk vtvasdglrl sstwrsdice klhpilfrlq egssvkplss
      541 i
//
LOCUS       XP_077514633             334 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125265 [Amblyomma americanum].
ACCESSION   XP_077514633
VERSION     XP_077514633.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658507.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 30% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..334
                     /product="uncharacterized protein LOC144125265"
                     /calculated_mol_wt=36093
     Region          <246..>320
                     /region_name="half-pint"
                     /note="poly-U binding splicing factor, half-pint family;
                     TIGR01645"
                     /db_xref="CDD:130706"
     CDS             1..334
                     /gene="LOC144125265"
                     /coded_by="XM_077658507.1:126..1130"
                     /db_xref="GeneID:144125265"
ORIGIN      
        1 mlkvlrllle irqqqreilq rlsrleqcpq lpaftiedfe aaeaavrder vaaalrkqlv
       61 rlggnnlkev avnvmsavmg vavqrlfslr grkgkrpfvg tklcrvkqqm psikpiisic
      121 rivirchelp cngrfdvdcs vfmiahffaf yyctvlilfv gipcvsvtcg fsasnhelgf
      181 pyacrkcivl kvaflglnah spctdaicer qgvdilaaqg figrwlpgac drgggrkrry
      241 aqafeppesh apseepqtls gsappvnpca gsvtrpgapt ascpatppgm aitspavahp
      301 ssapspalpp hsllltpshp tqhpchmdrc ptgt
//
LOCUS       XP_077514644             457 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077514644
VERSION     XP_077514644.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658518.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..457
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=50460
     Region          79..215
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          304..>412
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..457
                     /gene="LOC144125270"
                     /coded_by="XM_077658518.1:102..1475"
                     /db_xref="GeneID:144125270"
ORIGIN      
        1 mkirwfpvac allatiahas vgseasskne thgsnpgadv varwkaieag akslvqkvis
       61 ssyrealslv headlssdcf isltsvlrgl qelntdiirl ldswgkipsg fsegslaelg
      121 dydlclrlvv hktarsgrga plfrgqycsf tldpplpprp avirnskpvi dsssfanhta
      181 vkealdvswg lyttrlrmgi cvpdqcspde igrilqavsg kmhlsttllg cevqeelsln
      241 nsqivaiail gafiaavlta tacdlilgsl rdrgiykekq gaslkafltl svprttsavf
      301 tvgtsksslk vfnglricgv fwvvafhtyi ypdistyral relqknavyi pfqfinnawl
      361 cvdmfffisg fliiynqrki pakanlyrfy lkgliqrywr liplallvml ilfleplfgs
      421 gpiwkekits darncelrww svlggfsnff hidnttt
//
LOCUS       XP_077514655             201 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125279 [Amblyomma americanum].
ACCESSION   XP_077514655
VERSION     XP_077514655.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658529.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 40% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..201
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..201
                     /product="uncharacterized protein LOC144125279"
                     /calculated_mol_wt=23024
     CDS             1..201
                     /gene="LOC144125279"
                     /coded_by="XM_077658529.1:311..916"
                     /db_xref="GeneID:144125279"
ORIGIN      
        1 mrgglleklt tqlyatyhic gdhfttddfm dtgmtslkka aipavqptgc ldcqlakgcf
       61 sttisfltll rpprrrlsgr sssahreyra lssilqkdli dwcapldcst krcrklsavy
      121 alpytrvqkv etqlcrrrms rdrmgeqata pvrkrllwev grrwnrecdy ekwttdnhft
      181 snhcdflset glhsffelsi s
//
LOCUS       XP_077514666             494 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125286 [Amblyomma americanum].
ACCESSION   XP_077514666
VERSION     XP_077514666.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658540.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..494
                     /product="uncharacterized protein LOC144125286"
                     /calculated_mol_wt=54493
     CDS             1..494
                     /gene="LOC144125286"
                     /coded_by="XM_077658540.1:23..1507"
                     /db_xref="GeneID:144125286"
ORIGIN      
        1 mrslgvsvkg arlnhdaaps lflhkrsmpp pprsafakrr khevlaellg egssvekldp
       61 klgspsgspt dvtteagadl camrhenfgv enslsilalt dsahdatlev kltescegrh
      121 ksvqatmrpa tkstavqacv krsmashhsq tkphtvsigi qvgspminva tqttmpiqre
      181 aienddsted eggdedydde dytpceefee kqgcvgnspd epgnkiflvq ekylwklfql
      241 cteclaprtv rfecegtlle vygecasghf iywcnqdvek qqpmlnlqlc aavlfsgcnp
      301 tatlrmlasi gvpvvsnrtf faiqrsylwp aigrvrshce evssflcmvk vqctlfcfqv
      361 wkeeqksllq elqgqqvnla gdgradspgf sakygtytlm dverhkvlhf evvqsndagg
      421 scrmeleglk qglafleses ikvavlvtdr htqikcflrn nkaaiaheld vwhkqkvsss
      481 cgfmvadkvl fsay
//
LOCUS       XP_077514679             524 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125294 [Amblyomma americanum].
ACCESSION   XP_077514679
VERSION     XP_077514679.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658553.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..524
                     /product="uncharacterized protein LOC144125294"
                     /calculated_mol_wt=58763
     Region          19..113
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          274..>311
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          353..513
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..524
                     /gene="LOC144125294"
                     /coded_by="XM_077658553.1:309..1883"
                     /db_xref="GeneID:144125294"
ORIGIN      
        1 maavcdlrsn lwkgrgktcc vvdckncdrd lkvwdesvce lhgpelhkdc pclrpfamhc
       61 fpagernklv rqrwianlqr kdfnpgkaar vcsihfidgr ptpnnpyptl hlgtsavrhk
      121 grrvlkhrlv iapmvetsst gastshntsi qhsptqeeee slsetddtrp tatylrpars
      181 ngertaagcq tcgcrcetvk meqavqvdmk gdedhtycaa sspqrsteaa cqtaftvcst
      241 lqphtfkfft glsknlfdel vralastmkt pfelpfedqv lltlmrlrlg llvhdlsfhf
      301 aistaavsri fctissglak fsrqylvfwl ardtikasip dsfnryelst ciidcfeifi
      361 erpgnlkrrn rtysrykshn takvlhaiap cgfvmfaskp yggrasdrfi tqhsgflkfl
      421 apgdtiladr gftisdllpa gvklmlpsfs kgrsqisrpe lvesrrlarl rihversirr
      481 ikcfrllkel pssffakkpl vddilvvvag lcnlqpplik etlc
//
LOCUS       XP_077514691             420 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125301 [Amblyomma americanum].
ACCESSION   XP_077514691
VERSION     XP_077514691.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658565.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 46% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..420
                     /product="uncharacterized protein LOC144125301"
                     /calculated_mol_wt=46203
     CDS             1..420
                     /gene="LOC144125301"
                     /coded_by="XM_077658565.1:1..1263"
                     /db_xref="GeneID:144125301"
ORIGIN      
        1 masqqqqvda avtttadasp cdlisfepsg dsrdtlaqts gnarhfspsa mspgrestaq
       61 paqpcsshaa pqpelpgqan pqeavlrnal rliesltgiv qnnlqtpapv arprirvdlp
      121 syggyhdlvs aneyldrmln yqqatgltdg evlarvvpvs ltdhaarwfr ltghrsrtme
      181 efrvrfrefl padydrrlrr elelrtqhpd eslleyvram delyriadps apngekverv
      241 trqahptfaa ylrgvqfrdl dglasaakri qadilasray rppppasqal eprcawngdt
      301 frirsrgaet vafsdgqlrn gwelsdrald pytyamraah aarsqvedgg taapdeggca
      361 daatkagrrk tvqkpevsea sqprqraael kqrphgagsa rrkwahslpf sicmakrsse
//
LOCUS       XP_077514701             700 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125308 [Amblyomma americanum].
ACCESSION   XP_077514701
VERSION     XP_077514701.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658575.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 43% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..700
                     /product="uncharacterized protein LOC144125308"
                     /calculated_mol_wt=79455
     Region          82..167
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     Region          <250..286
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     Region          312..401
                     /region_name="Asp_protease_2"
                     /note="Aspartyl protease; pfam13650"
                     /db_xref="CDD:433378"
     Site            order(326,328,330,353..355,399)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133137"
     Site            326..328
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133137"
     Site            326
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133137"
     Site            order(353..356,360..363)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133137"
     Region          417..512
                     /region_name="rve"
                     /note="Integrase core domain; pfam00665"
                     /db_xref="CDD:459897"
     CDS             1..700
                     /gene="LOC144125308"
                     /coded_by="XM_077658575.1:1..2103"
                     /db_xref="GeneID:144125308"
ORIGIN      
        1 mrekdrllas llerlalpas sgapgvaaat ttyqvmpdls knisdfdgsg daasakewlg
       61 nlqttatlhr wpepfmleta kwhlvgpakd wlrsrkevst swenfdyhfr rtfcgqtraa
      121 erwrkmqerv qqrnesttay fhskvrlcre anldfcdtre qvltglrsrq lctmllgrth
      181 eddddllhdi meferidrer eqrfggtrek rqftaaterv aetpparktt ydrfgkdrtp
      241 pltnergqfk cyscgrfghi srdcpeerhe mkclrcgksg htqrhcsmsq snetntvtdt
      301 erkiapgall kqamlngttt lvgmidngss gcllrasaaa rcgarltkda talygfgsqg
      361 tpsaraigkc ytdleingvk genvpflvvp ddtltvdllv grtregllhp ippgkrpfev
      421 ihmdhmgpfv pskkrnqyll vfidnltrfv rliatrdtsa khvlraleef vlerglprtv
      481 isdrgscfts rafaefctar cirhvlnstr hpqanglver vnrtvlpvia tsmsdpkqrd
      541 wdeklskaer dinnavnkts gktpfemlyg yqpefhstal rrivgsdeaq wenpeelrtr
      601 vrqriiaeqe kskaeydrrh fparlydvgd ivfmtkapeq tgkptkaqph vsqlkgwtst
      661 hpqdrledde ssteeedtar evesvpqplr rserkravps
//
LOCUS       XP_077514736             282 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125328 [Amblyomma americanum].
ACCESSION   XP_077514736
VERSION     XP_077514736.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658610.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 23% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..282
                     /product="uncharacterized protein LOC144125328"
                     /calculated_mol_wt=31140
     CDS             1..282
                     /gene="LOC144125328"
                     /coded_by="XM_077658610.1:62..910"
                     /db_xref="GeneID:144125328"
ORIGIN      
        1 msisglappp pflpvpgrpa vawpqwfrif enyvlasgas dctpdrrkal llhslgvegq
       61 rifytlplps ldnvklgeqt atektsedsk gtgdtrpevs sydiavaalh dhfsatsnvv
      121 aerhrfsrrv qqagesvney italrelsat csfpveedsl rdqivagiss rslrerllle
      181 gsslsfarav llarqfeqay ndlqefpsvd vgrintrgnt ptpvptffvf arkvdalnnh
      241 lpelnyiptl lpnlpaylsk ttpdskapkk ktaarramry qm
//
LOCUS       XP_077514795             672 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125356 [Amblyomma americanum].
ACCESSION   XP_077514795
VERSION     XP_077514795.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658669.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..672
                     /product="uncharacterized protein LOC144125356"
                     /calculated_mol_wt=76069
     Region          11..103
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          345..>490
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..672
                     /gene="LOC144125356"
                     /coded_by="XM_077658669.1:1..2019"
                     /db_xref="GeneID:144125356"
ORIGIN      
        1 mekrkkadth cfspgcktgy pghrvengrk lsvfaapkda errkvwernl krddkplaet
       61 saicerhfee hfilrdyihv idgkeirlpr gkpalapdav ptlvpglpky lskpsskpra
      121 ertrrevaaa gprkksrlsr cdehenensd rssnsigdfk qaqkvvqtgd lhfiaenvtv
      181 pklwtrlntp dldtllyatt hatktpftvf hekvvwfssd gasgivakcy ingkekecre
      241 nnylheagdi lkeadcaric agamsaetyh elnemltfkm kntlnllnsk dfskfclgav
      301 taeggfcvsc rylrkalvtr ksklntktvk tnlsqrlrit sqktkrlslr lrnlaskitt
      361 mkeensktse atfqrriesl ttkqqeaalh mfkaskrksr rgmmyskvwi leciimrmkg
      421 prlyehmrrq niltlpskvt lrrylksyri gfgfnenvln ilkqktstmd ickrhggliv
      481 eemklsenlc vsssghiqgf vdmliftprs emhnvadhgm vimfvpfagk wtqilacfat
      541 rgnmkgdflt kvmleavtla enaglfvdfi tshgatwnrk mwtvmgiqat asstkckaqh
      601 pvdgkgflhf dsnfphvvkc vrngllhsgf ntpaghvvrr cadkeeeppq prpppankrq
      661 ekqssaekrf gs
//
LOCUS       XP_077514832             337 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125376 [Amblyomma americanum].
ACCESSION   XP_077514832
VERSION     XP_077514832.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658706.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..337
                     /product="uncharacterized protein LOC144125376"
                     /calculated_mol_wt=38186
     Region          172..322
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..337
                     /gene="LOC144125376"
                     /coded_by="XM_077658706.1:13..1026"
                     /db_xref="GeneID:144125376"
ORIGIN      
        1 mpksilqdpl llsrlkwsei edllclevfg etrrdplsvr glinidamds gvfrtyfrye
       61 kddlrklqsa lripkmvvtp qnvsvpgdea lcitfrrlay pnrlcdledl fgrhssalss
      121 ltndvlrhvd ecffhllddf nnhmwlnlnt lekfsqdiha kgapltncwg fidgttraic
      181 rpsrnqklyf sgnkrfhalk yqsimclngi icqldgsypg skhdagnfgi skvysklekl
      241 vqgqsyciyg dpayplrpll lkpyggvslt peqcefnkpi sgvrqavewg sgkivrlfaf
      301 vdfkknqklh lqnlpriyka salsanchtc lygaqkp
//
LOCUS       XP_077514870             282 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125391 [Amblyomma americanum].
ACCESSION   XP_077514870
VERSION     XP_077514870.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658744.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 44% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..282
                     /product="uncharacterized protein LOC144125391"
                     /calculated_mol_wt=30732
     CDS             1..282
                     /gene="LOC144125391"
                     /coded_by="XM_077658744.1:1..849"
                     /db_xref="GeneID:144125391"
ORIGIN      
        1 manadgnasa mpgdgpssav saqslrlpqf spadpqlwla lvnnqftvgr iisqaqkfhh
       61 vaaslppeia tevrdilinp pasapfdtla aelirraavs ehrrlqqlis seelgdrkpt
      121 qllrrlqqll gdkgatfdqa flrefflqll pssvrlvlvp aqglslekla elsdsvmdvs
      181 cstlslgavs mpatppsplp shvdlqslrd dfqaqftris dqiatlstsr sllpsrrras
      241 ssrrsatppl eastcwyhrt fgeaahrctp pcrfqgnfqr hh
//
LOCUS       XP_077514883             931 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125401 [Amblyomma americanum].
ACCESSION   XP_077514883
VERSION     XP_077514883.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658757.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..931
                     /product="uncharacterized protein LOC144125401"
                     /calculated_mol_wt=101892
     Region          220..485
                     /region_name="RT_nLTR_like"
                     /note="RT_nLTR: Non-LTR (long terminal repeat)
                     retrotransposon and non-LTR retrovirus reverse
                     transcriptase (RT). This subfamily contains both non-LTR
                     retrotransposons and non-LTR retrovirus RTs. RTs catalyze
                     the conversion of single-stranded RNA into...; cd01650"
                     /db_xref="CDD:238827"
     Site            order(310..315,369..370,402,404..405,481..482)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238827"
     Site            order(310..315,369,404)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238827"
     Site            370
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238827"
     Region          688..815
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     Site            order(692..695,725..727,730,755,794,812)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260008"
     CDS             1..931
                     /gene="LOC144125401"
                     /coded_by="XM_077658757.1:1..2796"
                     /db_xref="GeneID:144125401"
ORIGIN      
        1 mykvihwdkf rdllrsfslq ddilthvarc aeaaticcev psgqpipdll llqlraqrrq
       61 aerralrtdr pehwtlhnrl dakgsarawr lfrsllepsi qrapylaiav aggltyqqla
      121 dklaeallpr epppaglhpr rhcvqpacsh avditavcsr altkgelssa llhskrrsap
      181 gpdgvtyqml rnlddamqts llqtynevws tgrlpeawrt alvvpvlkag kppadllsyr
      241 pvsltsdpgk lmesmalgrl ewiaaatrfl peqqsgfrrh rctadaiadi vssledarhs
      301 hhvvcltlld vkgafdnvyh eaileavncl gvtgnlrgyi qgflrdrtsc vraggatstp
      361 qaltrgvpqg svlspmlfnl vmaqlptclp dglklpvria iyaddialwc rgprrqrdqv
      421 iknvqraitr vgsylervgl qlsptkseam llnirpgarp gktylsvagr elpwrhscry
      481 lgllidrhlt wrpavkafcr qatrvrgair sllaggngit pqmglrffqa magaqflyal
      541 plvqmtiqqq laiersqraa vrlclglprg shisatlaea gvwpmmlqaq rtalrhaerl
      601 hlapdgrlll erllghphsr mgkvaqefeq lvggqpehlp spprpdlghq frvlmpprgt
      661 rpkrltpavg lrqeaaatle edlagctqly tdgsvlmgac psaaaactap slgesrqarl
      721 pfvassttae laalhlaadi leshpalqtv ailsdsrval qllqeprsrl pivrklaarl
      781 dsicshgcsi slawipshvg vpgneeadal akaahghrtp cstavvpldv argiaerqll
      841 mehpdprvaq gkppqrlpdk gitrplrsqv legrifglqm srtvafpskv lngkratfvp
      901 rictpyafgy ndliiigpcg arvaealygq e
//
LOCUS       XP_077514927             615 aa            linear   INV 25-NOV-2025
DEFINITION  gamma-glutamyl phosphate reductase-like [Amblyomma americanum].
ACCESSION   XP_077514927
VERSION     XP_077514927.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658801.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 24% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..615
                     /product="gamma-glutamyl phosphate reductase-like"
                     /calculated_mol_wt=67119
     Region          <23..60
                     /region_name="MDR"
                     /note="Medium chain reductase/dehydrogenase
                     (MDR)/zinc-dependent alcohol dehydrogenase-like family;
                     cl16912"
                     /db_xref="CDD:450120"
     Region          <154..613
                     /region_name="PLN02418"
                     /note="delta-1-pyrroline-5-carboxylate synthase"
                     /db_xref="CDD:215230"
     CDS             1..615
                     /gene="LOC144125429"
                     /coded_by="XM_077658801.1:1..1848"
                     /db_xref="GeneID:144125429"
ORIGIN      
        1 mdqpgcggmm snvgsdggnv tswksvdqvp qlvelylnkk lklddlithr vhlerindaf
       61 tfrqgslsqp fhardgpvtg qwqtasadsr nytswmyspr arenllpfes vhsktekrvi
      121 gtfclrgale krcvhmvhds lnnqnawnvn lcfqikaasw alehvvsvvi gsgfeemait
      181 rivqgkrvgt fftehqskmi slestaanaw kasrtleslt atqrsaavla madalearte
      241 dimeanerdl tvareqglsa pmlarlaltp amlsaladsl rtiaktspsv lgrvlrktrv
      301 aegmelsqvt vpigvllvif earpdclpqv aalavatang lllkggheaa htnrclwqll
      361 qqclkpydaa dalalastre dvdellhleg yidlviprgs nqlvrdiqre srgipvlghs
      421 dgichvyvdr eadkqkamri vveskcdypa acnametllv hrdhveggll selcsslkth
      481 gvslysgprm aqcngpqsad evvdsvdeal ehinthgssh tdaivtknkd taerfprgay
      541 sacvfhncst rfadgyrfgl gaevdistgr ihargpvgme gllttkwllr gsgnvvqdfg
      601 esgtrtylhe nlpgs
//
LOCUS       XP_077514939             625 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077514939
VERSION     XP_077514939.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658813.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..625
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=70844
     Region          <46..125
                     /region_name="PAZ"
                     /note="PAZ domain; pfam02170"
                     /db_xref="CDD:460472"
     Region          152..581
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(284,288,300..303,306,315,318,322,326)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(353,355,431,571)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..625
                     /gene="LOC144125439"
                     /coded_by="XM_077658813.1:1..1878"
                     /db_xref="GeneID:144125439"
ORIGIN      
        1 mhaarrgdta dprswrpsys rrrcqdqanh fsieiptgsi yqydvdisse iaqetkeiyf
       61 esegsqisva dyfqsryrrl sypkfpcvqs gspthpvyip levcelaegq hcrkkldesq
      121 taemikrtsk ppakrfqeir qsvrdmvsss dkylrefgvk inteptqvvg rvldppslvf
      181 enntmckprd gtwdlrgqrf ykamsmtkwi vlnvsrfahk dslenfirml irigqelgmr
      241 iaqplavitf dtirqpmrtv laeqrkqhpq lemvvavitk atnyaeikqv aetelslrtq
      301 cildnnvvqk cnaaliqnlc qkinakmggi nnsllmqekp klfhrpvivi gadvshpspg
      361 ekvrpsiaac vgsldtvpsk fhatirvqie dseakarvei irdlkdmike lllafhratr
      421 qkperivfyr dgvsegqfle vrnhevsair lackelspne tyepaltfiv vqkrhhtrfm
      481 pandrdgvgk crnvppgttv dsvvthpldf dfflcshfgi kgtsrpahyy ivwddskfsa
      541 ddlqklsfyl chtysrcars vsvpapvyya hlaafrakqh iaskletsgv vreppegggd
      601 avlttaqyve avkvlqalqa smyfv
//
LOCUS       XP_077514951             183 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-4-like [Amblyomma americanum].
ACCESSION   XP_077514951
VERSION     XP_077514951.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658825.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="protein argonaute-4-like"
                     /calculated_mol_wt=20180
     Region          <4..139
                     /region_name="Piwi-like"
                     /note="PIWI domain. Domain found in proteins involved in
                     RNA silencing. RNA silencing refers to a group of related
                     gene-silencing mechanisms mediated by short RNA molecules,
                     including siRNAs, miRNAs, and heterochromatin-related
                     guide RNAs. The central component...; cl00628"
                     /db_xref="CDD:412485"
     CDS             1..183
                     /gene="LOC144125447"
                     /coded_by="XM_077658825.1:1..552"
                     /db_xref="GeneID:144125447"
ORIGIN      
        1 mkgvsairla ckelspnety epaitfivvq krhhtrfmpa ndrdgvgkcr nvppgttvds
       61 vvthpldfnf flcshfgiqg tsrpahyyvv wddsmfsadd lqklsfylch tysrcarsvs
      121 ipapvyyahl aafrakhhia skletsgavs qssegggdtv lttaqyveav kvlqelqasm
      181 yfv
//
LOCUS       XP_077514962            1391 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077514962
VERSION     XP_077514962.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658836.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 38% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1391
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=155756
     Region          31..69
                     /region_name="zf-C3HC4_3"
                     /note="Zinc finger, C3HC4 type (RING finger); pfam13920"
                     /db_xref="CDD:464042"
     Region          298..1056
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          688..1057
                     /region_name="Piwi-like"
                     /note="PIWI domain. Domain found in proteins involved in
                     RNA silencing. RNA silencing refers to a group of related
                     gene-silencing mechanisms mediated by short RNA molecules,
                     including siRNAs, miRNAs, and heterochromatin-related
                     guide RNAs. The central component...; cl00628"
                     /db_xref="CDD:412485"
     Site            order(820,824,836..839,842,851,854,858,862)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     CDS             1..1391
                     /gene="LOC144125455"
                     /coded_by="XM_077658836.1:1..4176"
                     /db_xref="GeneID:144125455"
ORIGIN      
        1 maptslqtvf gfdrgfdwrp tcfvnpfppt rvcivcglvp ssaamlpclh llcqscsega
       61 gakrnhcpfh kepfqeedvv wsaftrdsil grkvrcwnae hgcdaevvas amlehfanac
      121 qfhvppkfse lttgveaala edqreviaei rdalayiqrt mkeknsegsv psasggnqat
      181 ppapspagps wvsfaqalqq gaaaspqpra vsggpttaad psahptaqps asrpsygaaa
      241 aagsakvass sprpasaaaa vsgtqpaaaa lppsppstpp vavtqptpaa gdqatsgdvv
      301 rikelertlp shfprrpahg qlgrtiqlla nhfsieiptg svyhydvdis setaketkvp
      361 eqkkyrclst kinriviell vkkyrqdlan cipafdgrkn lytrrqlnfr ertftvdlee
      421 dqrsqkfiik iqyaatvnld alhgvfqkrv qtvpqevlqa idivlrhsps inlapvgrsf
      481 frppgpnehn dlgggreawf gyytsvrpaq wkpmlnvdms attfyeslpl vdfmcrflsd
      541 srrvltpadf rslrdnqyvr lnrelkglrv kvthlpyprk ykvvkitrep akeiyfeseg
      601 sqisvadyfq sryrrlsypn fpcvqsgspt hpvyiplevc elaegqhcrk kldesqtadm
      661 ikrtakppak rfqkirqsvr dmvsssdkyl refgvkinte ptqvvgrvld ppslvfennt
      721 mckprdgtwd lrgqrfykam smtkwivlnv srfahkdsle nfirmlirig qelgmriaqp
      781 lavitfdtnr qpmrtvlteq rkqypqlemv vavitkatny aeikqvaete lslrtqcild
      841 nnvvqkcnaa liqnlcqkin akmgginnsl lmqekpklfh rpvivigadv shpspgdkvr
      901 psiaacvgsl dtvpfkfhat irvqiedska ktrveiirdl kdmikellla fhratrhkpe
      961 qivfyrdgvs egqflevrny evsairlack elspnetyep aitfivvqkr hhtrfmpand
     1021 rdgvgkcrnv ppgttvdsvv thpldfdffl cshfgiqswr hsvcqpcydg aaakrshcpl
     1081 dkepfqeedm awttisrgnv lgqkvrrcns ehgcdaecva samlehfant yqyhamispc
     1141 swrhsvcqpc ydgaaakrsh cpldkepfqe edmawttisr gnvlgrkvrr wnsehgcdae
     1201 cvasamlehf antyqyhami spcpcypvyt ssaspaprap vpkrnhcpfh kepfqeedvv
     1261 wsaftrdsil grkvrcwnae hgcdaevvas amlehfanac qfhvvrcpec sesvrhrdia
     1321 ehlardcepp rardqasedn fsnafmevkr tlakywkkml pcarrwirsr tvctlipvap
     1381 lqrcapvspt q
//
LOCUS       XP_077514974             937 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-4-like [Amblyomma americanum].
ACCESSION   XP_077514974
VERSION     XP_077514974.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658848.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 35% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..937
                     /product="protein argonaute-4-like"
                     /calculated_mol_wt=106292
     Region          18..148
                     /region_name="PAZ"
                     /note="PAZ domain; pfam02170"
                     /db_xref="CDD:460472"
     Region          175..>487
                     /region_name="Piwi-like"
                     /note="PIWI domain. Domain found in proteins involved in
                     RNA silencing. RNA silencing refers to a group of related
                     gene-silencing mechanisms mediated by short RNA molecules,
                     including siRNAs, miRNAs, and heterochromatin-related
                     guide RNAs. The central component...; cl00628"
                     /db_xref="CDD:412485"
     Site            order(307,311,323..326,329,338,341,345,349)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Region          <754..893
                     /region_name="Piwi-like"
                     /note="PIWI domain. Domain found in proteins involved in
                     RNA silencing. RNA silencing refers to a group of related
                     gene-silencing mechanisms mediated by short RNA molecules,
                     including siRNAs, miRNAs, and heterochromatin-related
                     guide RNAs. The central component...; cl00628"
                     /db_xref="CDD:412485"
     CDS             1..937
                     /gene="LOC144125463"
                     /coded_by="XM_077658848.1:707..3520"
                     /db_xref="GeneID:144125463"
ORIGIN      
        1 mlnvdmsatt fyeslplvdf mcrflsdsrr vltpadfrsl rdnqyvrlnr elkglrvkvt
       61 hlpyprkykv vkitrepake iyfesegsqi svadyfqsry rrlshpnfpc vqsgspthpv
      121 yillevcela egqhcrkkld esqtaemikr takppakrfq kirqsvrdmv sssdkylref
      181 gvkinteptq vvgrvldpps lvfenntmck prdgtwdlrg qrfykamsmt kwivlnvsrf
      241 ahkdslenfi rmlirigqel gmriaqplav itfdtnrqpm rtvlteqrkq ypqlemvvav
      301 itkatnyaei kqvaetelsl rtqcildnnv vqkcnaaliq nlcqkinakm gginnsllmq
      361 ekpklfhrpv ivigadvshp spgdkvrpsi aacvgsldtv pskfhatirv qiedskakar
      421 veiirdlkdm ikelllafhr atrhkpeqiv fyrdgvsegq flevrnyevs airlackels
      481 pnetyepswr hsvcqpcydg aaakrshcpl dkepfqeedm awttisrgnv lgrkscsega
      541 gakrnhcpfh kepfqeedvv wsaftrdsil grkvrcwnae hgcdtevfas amlehfanac
      601 qfhvswrhsv cqpcydgaaa krshcpldke plqeedmawt tisrgnvlgr kvrrcnsehg
      661 cdaecvasam lehfantyqy hamispcscs egpgakrnhc pfhkepfqee dvvwsaftrd
      721 silgrkvrcw naehgcdaev vasamlehfa nacqfhvvsa irlackelsp netyepaitf
      781 ivvqkrhhtr fmpandrdgv gkcrkvppgt tvdsvvthpl dfdfflcshf giqgtsrpah
      841 yyvvwddsmf saddlqklsf ylchtysrca rsvsipapvy yahlaafrak hhiasklets
      901 gavsqssegg gdtvlttaqy veavkvlqel qasmyfv
//
LOCUS       XP_077514987             744 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-4-like [Amblyomma americanum].
ACCESSION   XP_077514987
VERSION     XP_077514987.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658861.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 15% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..744
                     /product="protein argonaute-4-like"
                     /calculated_mol_wt=82936
     Region          230..>704
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     CDS             1..744
                     /gene="LOC144125470"
                     /coded_by="XM_077658861.1:1..2235"
                     /db_xref="GeneID:144125470"
ORIGIN      
        1 mlpclhllcq scsegagakr nhcpfhkepf qeedvvwsaf trdsilgrkv rcwnaehgcd
       61 aevvasamle hfanacqfhv vrcpecsesv rhqdiaehla pvpsasggnq atppasspag
      121 pswvsfaqal qqgaaaspqp ravsggptta adpsahptaq psasrpsyga aaaagsakva
      181 sssprpasaa aavsgtqpaa aalppsppst ppvavtqqtp aagdqatsgd dvrikelert
      241 lpshfprrpa hgqlrrtiql lanhfsieip tgsvyhydvd issetaketk vpeqkkyrcl
      301 stkinrivie llvkkyrqdl ancipafdgr knlytrrqln frertftvdl eedqrsqkfi
      361 ikiqyaatvn ldalhgvfqk rvqtvpqevl qaidivlrhs psinlapvgr sffrppgpne
      421 yndlgggrev wfgyytsvrp aqwkpmlnvd msattfyesl plvdfmcrfl sdsrrvltpa
      481 dfrslrdnqy vrlnrelkgl rvkvthlpyp rkykvvkitr epakeiyfes egsqisvady
      541 fqsryrrlsy pnfpcvqsgs pthpvyiple vcelaegqhc rkkldasqta emikrtakpp
      601 akrfqkirqs vrdmvsssdk ylrefgvkin teptqvvgrv ldppslvfen ntmckprdgt
      661 wdlrgqrfyk amsmtkwivl nvsrfahkds lenfirmlir saknwacalr srlrssrstq
      721 paspcgpfsr ssasstrswr wlwq
//
LOCUS       XP_077514997             304 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077514997
VERSION     XP_077514997.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658871.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 42% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..304
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=33717
     Region          <26..68
                     /region_name="PEX10"
                     /note="RING-finger-containing E3 ubiquitin ligase
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG5574"
                     /db_xref="CDD:227861"
     Region          <121..260
                     /region_name="Piwi-like"
                     /note="PIWI domain. Domain found in proteins involved in
                     RNA silencing. RNA silencing refers to a group of related
                     gene-silencing mechanisms mediated by short RNA molecules,
                     including siRNAs, miRNAs, and heterochromatin-related
                     guide RNAs. The central component...; cl00628"
                     /db_xref="CDD:412485"
     CDS             1..304
                     /gene="LOC144125477"
                     /coded_by="XM_077658871.1:1..915"
                     /db_xref="GeneID:144125477"
ORIGIN      
        1 maptslqtvf gfdrgfdwrp tcfvnpfppt rvcivcglvp ssaamlpclh llcqscsega
       61 gakrnhcpfh kepfqeedvv wsaftrdsil grkvrcwnae hgcdtevfas amlehfanac
      121 qfhvvsairl ackelspnet yepaitfivv qkrhhtrfmp andrdgvgkc rnvppgttvd
      181 svvtrpldfd fflcshfgiq gtsrpahyyv vwddsmfsad dlqklsfylc htysrcarsv
      241 sipapvyyah laafrakhhi askletsgav sqssegggdt vlttaqyvea vkvlqelqas
      301 myfv
//
LOCUS       XP_077515012             504 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125484 [Amblyomma americanum].
ACCESSION   XP_077515012
VERSION     XP_077515012.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658886.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 12% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..504
                     /product="uncharacterized protein LOC144125484"
                     /calculated_mol_wt=55911
     CDS             1..504
                     /gene="LOC144125484"
                     /coded_by="XM_077658886.1:117..1631"
                     /db_xref="GeneID:144125484"
ORIGIN      
        1 msayyepsay tqaygqygyd qtaygqttyd ytqattqalt skpedtkpts qyrmtstmvi
       61 tcilaivial vafiallvva lgtgfgigae tdvpedmdrp plpsvypvvr vpipssvmtt
      121 tatdtsitpt astqsprtvm dqhplvctmg srtsflqmfp pdglceyiff dsvekgnrnp
      181 lafpkrwgnd lrifitayar ynttafgigf aydssllesf wrknifhfgi vdtatrnare
      241 advelalnvl kalnthaetq rrqgnpsfif fagvvfnqnw tnfyndkftn iykphlfiaq
      301 ghffygdntf sdcrvmppta ptrpqglggs yqhdltlaad sfsklssrgl siigalsitm
      361 kgrwtrtlpv qgpsdflsgc vhntsapsfg ryseicrnpk ylqrsysqth dailavhdtd
      421 nvlfaydnev glcrklcavk gthsgvrfgi avydleyedf snacgvfnky gafsrvhair
      481 gilnyfktrf qsapdavgcr elit
//
LOCUS       XP_077515043             262 aa            linear   INV 25-NOV-2025
DEFINITION  alcohol dehydrogenase class-3-like [Amblyomma americanum].
ACCESSION   XP_077515043
VERSION     XP_077515043.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658917.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..262
                     /product="alcohol dehydrogenase class-3-like"
                     /calculated_mol_wt=28236
     Region          <46..260
                     /region_name="MDR"
                     /note="Medium chain reductase/dehydrogenase
                     (MDR)/zinc-dependent alcohol dehydrogenase-like family;
                     cl16912"
                     /db_xref="CDD:450120"
     Site            order(61,65,86..91,110..111,115,130,155..156,179..180,
                     204..206)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:176178"
     CDS             1..262
                     /gene="LOC144125486"
                     /coded_by="XM_077658917.1:1..789"
                     /db_xref="GeneID:144125486"
ORIGIN      
        1 mdrnkhkgsp lceeenagda aasvtqrplh wgllrdelce arrltqisia apldkvwllc
       61 cavsrgydaa lnasmitkes kvgiwglgav glsavmgake rgaktiigid ineanfpkak
      121 efgctecvnp ntlsktivqh laeitnggld itfecignvs tmrdalesch agwgesfilg
      181 aaptdevist lpsqltagra wrgcafggwk svdqvpqlvv elyldkklkl ddliphrvhl
      241 erindaftfm qgglsirtai sf
//
LOCUS       XP_077515057             129 aa            linear   INV 25-NOV-2025
DEFINITION  all-trans-retinol dehydrogenase [NAD(+)] ADH4-like [Amblyomma
            americanum].
ACCESSION   XP_077515057
VERSION     XP_077515057.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658931.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 35% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..129
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..129
                     /product="all-trans-retinol dehydrogenase [NAD(+)]
                     ADH4-like"
                     /calculated_mol_wt=13439
     Region          <2..>56
                     /region_name="MDR"
                     /note="Medium chain reductase/dehydrogenase
                     (MDR)/zinc-dependent alcohol dehydrogenase-like family;
                     cl16912"
                     /db_xref="CDD:450120"
     CDS             1..129
                     /gene="LOC144125490"
                     /coded_by="XM_077658931.1:87..476"
                     /db_xref="GeneID:144125490"
ORIGIN      
        1 msrgygaaln aamitkeskv giwglgavgl savmgakerg aktiigidin eakfqkegca
       61 gvmprrlgrv lhpgssthgq gdlhsalaah rrpglarmrl rsftggalrl svavmrrpse
      121 eapvrteap
//
LOCUS       XP_077515066             168 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125499 [Amblyomma americanum].
ACCESSION   XP_077515066
VERSION     XP_077515066.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658940.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 40% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..168
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..168
                     /product="uncharacterized protein LOC144125499"
                     /calculated_mol_wt=18741
     Region          136..155
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..168
                     /gene="LOC144125499"
                     /coded_by="XM_077658940.1:260..766"
                     /db_xref="GeneID:144125499"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrppfa altsvlccqr pasigpcvlp afgcvippdp
       61 fmlktkscme ivqatklrvi fargleqaek lvdgqwysls kasfffsvpm eatwlhvgdl
      121 nrhlrsihlq vkplmcghch kkfakeatli rhmrtshrqm llqsvlka
//
LOCUS       XP_077515080             548 aa            linear   INV 25-NOV-2025
DEFINITION  delta-1-pyrroline-5-carboxylate synthase-like [Amblyomma
            americanum].
ACCESSION   XP_077515080
VERSION     XP_077515080.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658954.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..548
                     /product="delta-1-pyrroline-5-carboxylate synthase-like"
                     /calculated_mol_wt=59484
     Region          <72..546
                     /region_name="PLN02418"
                     /note="delta-1-pyrroline-5-carboxylate synthase"
                     /db_xref="CDD:215230"
     CDS             1..548
                     /gene="LOC144125505"
                     /coded_by="XM_077658954.1:1..1647"
                     /db_xref="GeneID:144125505"
ORIGIN      
        1 mdytswmysp rarenllpfe svhcktekrv iasvvsaiec yfflccnvll tgfyhaectc
       61 vqnvwnvnlc fqikaaswal ehgvsvvigs gfdemaitri vqgkrvgtff tehqskmisl
      121 estaanarka srtldsltat qrsaavlama daleartadi meanerdltv areqglsapm
      181 larlgltpak lsaladglrt iaktspsvlg lvlrktrvae gmelsqvtvp igvllvifea
      241 rpdclpqvaa lavatangll lkggdeaaht nrclwqllqq clkpydaada lalastredv
      301 dellhlegyi dlviprgsnq lvrdiqresr gipvlghsdg ichvyvdrea dkqkamrivv
      361 dskcdypaac nametllvhr dhveggllce lcsslkahgv slysgsrmvq lltfgppaap
      421 smrieysrpq cavevvdsvd ealehtnthg sshtdaivte nkdtaerflr vadsacvfhn
      481 cstrfadgyr fglgaevgis tgrihargpv smegllttkw llrgsgnvaq dyeasgnmty
      541 lhenlpas
//
LOCUS       XP_077515094             491 aa            linear   INV 25-NOV-2025
DEFINITION  delta-1-pyrroline-5-carboxylate synthase-like [Amblyomma
            americanum].
ACCESSION   XP_077515094
VERSION     XP_077515094.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077658968.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..491
                     /product="delta-1-pyrroline-5-carboxylate synthase-like"
                     /calculated_mol_wt=52715
     Region          <16..489
                     /region_name="PLN02418"
                     /note="delta-1-pyrroline-5-carboxylate synthase"
                     /db_xref="CDD:215230"
     CDS             1..491
                     /gene="LOC144125508"
                     /coded_by="XM_077658968.1:1..1476"
                     /db_xref="GeneID:144125508"
ORIGIN      
        1 mnltttpdnq nnfgsikaas waldhgmsvv igsgfeemai trivqgkrvg tfftehqskm
       61 islestatna rkasrtlesl tatqrsatll aiadaleart adimkanerd ltvareqgrs
      121 apmlarlalt paklsaladg lrtiaktsps vlgrvlrktr vaegmelsqv tvpigallvi
      181 feahpdcllq vaalavatan glllkgghea ahtnrclwql lqqclkpyda adalalvsts
      241 edvdellhlq gyidlviprg snqlvrdiqr esrgipvlgh sdgichvyvd readkqkamr
      301 ivvdskcdyp aacnametll vhrdhveggl lselcsslka qgvslysgpr mvqlltfglp
      361 aapcmrieys gpqcsvevvd svdealedin thgcshsdai vtenkdtaqr flrgadsacv
      421 fhncstrfad gyrfglgaev gistgrihar gpvgmegllt tkwllrgsgn vvqdfgesgt
      481 mnylhenlpa s
//
LOCUS       XP_077515131             342 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125525 [Amblyomma americanum].
ACCESSION   XP_077515131
VERSION     XP_077515131.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659005.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 47% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..342
                     /product="uncharacterized protein LOC144125525"
                     /calculated_mol_wt=38752
     CDS             1..342
                     /gene="LOC144125525"
                     /coded_by="XM_077659005.1:1..1029"
                     /db_xref="GeneID:144125525"
ORIGIN      
        1 megpsappae spvrdkdqcp vcqaefvleq srrrhvqkqh peaasslsgs akasyscdqc
       61 tnvfarrgql lqhheqnhgf vsrlsthqfs sysdfaawke meemkekvkf masswpkrws
      121 sgvtqmylwn rsgmhvktge gkrqpklvgs vkcnkhcfat mavtqegenv tveyqadhyg
      181 hdksvkhqrl tstqnaiaak lslgvpskri lldahatggd elnvlhtlnr hdvynvkhkf
      241 higyeeqkdp defvsmrmwi delqetsall yadkellteq gigkfkmalv nstqkeflle
      301 yagkidrnhd hqpyspalhc nrkakewqsc vkvchcqall gl
//
LOCUS       XP_077515208             304 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125585 [Amblyomma americanum].
ACCESSION   XP_077515208
VERSION     XP_077515208.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659082.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 32% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..304
                     /product="uncharacterized protein LOC144125585"
                     /calculated_mol_wt=32170
     CDS             1..304
                     /gene="LOC144125585"
                     /coded_by="XM_077659082.1:7..921"
                     /db_xref="GeneID:144125585"
ORIGIN      
        1 mssavessai sgnegpdgpe vrlplplrlp sppprlpspp prlpspppql pspprlispp
       61 sllshsmeaa tpaganctag cpassastag dledveeats dptmlkvlrl lleirqqqre
      121 ilqrlsrleq arqeprtrsp sppvsslppl cpqppaftie dfeaaeaavr ddrvaaalkk
      181 qllrlggnnl kevaanvmga vmgvavqrlf slrgrkgkrp fvgtklcrva tdaicerqgv
      241 dilaaqgfig rwlpgacdrg ggrkrryaqa feppeshaps eepqvppvnp cagdvrailg
      301 iqig
//
LOCUS       XP_077515221              85 aa            linear   INV 25-NOV-2025
DEFINITION  DNA topoisomerase 2 top-2-like [Amblyomma americanum].
ACCESSION   XP_077515221
VERSION     XP_077515221.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659095.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..85
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..85
                     /product="DNA topoisomerase 2 top-2-like"
                     /calculated_mol_wt=9563
     Region          <7..79
                     /region_name="TopoII_MutL_Trans"
                     /note="MutL_Trans: transducer domain, having a ribosomal
                     S5 domain 2-like fold, conserved in the C-terminal domain
                     of type II DNA topoisomerases (Topo II) and DNA mismatch
                     repair (MutL/MLH1/PMS2) proteins. This transducer domain
                     is homologous to the second...; cl02783"
                     /db_xref="CDD:445919"
     Site            60
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:238202"
     CDS             1..85
                     /gene="LOC144125588"
                     /coded_by="XM_077659095.1:1..258"
                     /db_xref="GeneID:144125588"
ORIGIN      
        1 mvlsasgefr qmsfvsdiat tkgcsnvdcv adhlvkkikd yqwmfvncli enptfdsqtk
       61 esitllakkf gsnyelsekv ynhvy
//
LOCUS       XP_077515234             447 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125595 [Amblyomma americanum].
ACCESSION   XP_077515234
VERSION     XP_077515234.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659108.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 35% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..447
                     /product="uncharacterized protein LOC144125595"
                     /calculated_mol_wt=46328
     Region          <160..216
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     CDS             1..447
                     /gene="LOC144125595"
                     /coded_by="XM_077659108.1:1..1344"
                     /db_xref="GeneID:144125595"
ORIGIN      
        1 merpcpavap yegavfpvys agflftvpep vpadhlidil eevvggmtyl qhlgglrfla
       61 amasmaqvsn tlargsllia gkdvpleqmg vpvvdvtvyr lppcvsdadl iaaftphgka
      121 flsrnrigng trlvklemtk sppnfltvae tramieyrgm rrvcsrcgge ghfgaacksv
      181 rcgrcgaygh vgadcrpvck rcggahptpd cirprsyaaa vasaaakapq epgdeprlse
      241 ssqaagavss gaaeeavtsl aepavqrdda vtspaddqag kelasssssq saaeqtegts
      301 daeksasvds ddslgdssle eppsldacll dvptaaqhyd teadtespas pspsddlhtp
      361 vndaqgsdss seprpqaphi pvalpprgpc vakvragark vhdledpkrq lssssegnap
      421 ssatsapkka rtadddddvl mdvtgne
//
LOCUS       XP_077515299             114 aa            linear   INV 25-NOV-2025
DEFINITION  uridine phosphorylase 1-like [Amblyomma americanum].
ACCESSION   XP_077515299
VERSION     XP_077515299.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659173.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..114
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..114
                     /product="uridine phosphorylase 1-like"
                     /calculated_mol_wt=12692
     Region          <16..106
                     /region_name="NP-I"
                     /note="nucleoside phosphorylase-I family; cl00303"
                     /db_xref="CDD:444819"
     CDS             1..114
                     /gene="LOC144125622"
                     /coded_by="XM_077659173.1:1..345"
                     /db_xref="GeneID:144125622"
ORIGIN      
        1 maclhshcat sfrrpgqarl dgtichysee dkfaflerlr dlgvvnieme ssqfaamrhh
       61 agvkgavlct tlldrtqgdq vdvrkdvmat ewqqrpqlla ihfmahrlgv alca
//
LOCUS       XP_077515315             318 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125623 [Amblyomma americanum].
ACCESSION   XP_077515315
VERSION     XP_077515315.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659189.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..318
                     /product="uncharacterized protein LOC144125623"
                     /calculated_mol_wt=35288
     Region          <32..>236
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..318
                     /gene="LOC144125623"
                     /coded_by="XM_077659189.1:1..957"
                     /db_xref="GeneID:144125623"
ORIGIN      
        1 msysadpvtp lmddkavqtd egaaaereae gqlaaagvel eslrclleem ssqlacevqa
       61 rrdaedyaqq aheelstvse kvaeevahss nlqqrieqve rdagealqha tselgqeleq
      121 lrrhmqehel slrkqlddkd kalrgaelsl halkedlere vavrkaaeer csklameled
      181 ttesaceakq slrnmqscle kanaklpdcr kelaarelss hkaamelvea keeelrclrp
      241 aadennplpt qrttrtsrat rmtsstrkea tdresdlgtr aqrprrvkks mavskdplcd
      301 nflqespknl saspgnap
//
LOCUS       XP_077515326             342 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125630 [Amblyomma americanum].
ACCESSION   XP_077515326
VERSION     XP_077515326.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659200.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 47% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..342
                     /product="uncharacterized protein LOC144125630"
                     /calculated_mol_wt=38738
     CDS             1..342
                     /gene="LOC144125630"
                     /coded_by="XM_077659200.1:23..1051"
                     /db_xref="GeneID:144125630"
ORIGIN      
        1 megpsappae spvrdkyqcp vcqaefvleq srrrhvrkqh petasslsgs akasyscdqc
       61 tnvfgrrgql lqhhkqnhgf vsrlsthqfs sysdfaalke ieemkekvkf iasswpkrws
      121 sgvtqmylwn rsgmhvktge gkrqpklvgs vkcnkhcfat mavtqegenv tveyqadhyg
      181 hdksvkhqrl tstqnaiaak lslgvpskri lldahatggd elnvlhtlnr hdvynvkhkf
      241 higyeeqkdp defvsmrmwi delqetsall yadkellteq gignfkmavm nstqkeflle
      301 yagkidrnhd hqpyspalhc nrkakewqsc vkvchcqall gl
//
LOCUS       XP_077515358             152 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125651 [Amblyomma americanum].
ACCESSION   XP_077515358
VERSION     XP_077515358.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659232.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 29% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..152
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..152
                     /product="uncharacterized protein LOC144125651"
                     /calculated_mol_wt=16892
     Region          <3..45
                     /region_name="GT1"
                     /note="myb-like, SANT family; cl23759"
                     /db_xref="CDD:474047"
     CDS             1..152
                     /gene="LOC144125651"
                     /coded_by="XM_077659232.1:608..1066"
                     /db_xref="GeneID:144125651"
ORIGIN      
        1 mnrwknlkdt frkkikdead skrsgaaagi ksswpymqqm eflrdtvetr rsfcnlaptd
       61 lsvgsaelhm aspvsnekep etpldeleai fggpgytegs eagllpaptq stklqavfnr
      121 eliresepps mlfevmqyth kriligngtm lv
//
LOCUS       XP_077515372             248 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125654, partial [Amblyomma
            americanum].
ACCESSION   XP_077515372
VERSION     XP_077515372.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659246.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         <1..248
                     /product="uncharacterized protein LOC144125654"
     CDS             1..248
                     /gene="LOC144125654"
                     /coded_by="XM_077659246.1:<1..747"
                     /db_xref="GeneID:144125654"
ORIGIN      
        1 lfqrhfglar sfggdeshpt vmnfsqifrl lslctpikta lrgsvqgepn avlvsveetl
       61 ktaaeahrsn kaslrneiea rllemttfpc vtpdqmsdgh syfrgspdds vvyylsgyvv
      121 hkvsnrtecq lclhdigsaa pvvgsdaylt ryrsfkegsl rhpsikmlhf vrvvnesvsl
      181 sldeeglcgd lfwkvlnelg cdqhkptftc qvlyffivtr mhfyardvnr rlqtrekvai
      241 askkarll
//
LOCUS       XP_077515402             278 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125680 [Amblyomma americanum].
ACCESSION   XP_077515402
VERSION     XP_077515402.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659276.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..278
                     /product="uncharacterized protein LOC144125680"
                     /calculated_mol_wt=29741
     CDS             1..278
                     /gene="LOC144125680"
                     /coded_by="XM_077659276.1:1..837"
                     /db_xref="GeneID:144125680"
ORIGIN      
        1 miyaskikln vqnldlaplr qfpseglqqr krpydeeslv aqstrilprs edsaggrpkd
       61 kegatgpeqp angssgrpga gasssvqgrt cgetaaraka adqrrkaagr enraatgplp
      121 satetaaags tpsatrrrpg vchqrderep hkdvdlfeat steclqtgrp iineaeatld
      181 asdfttatqe dgtgfsspip tpspasstss gpqkrkrghd kafkkeigil gelaskrada
      241 selfglfmgd khrrcpqrlr gqfemellqv aaryvdne
//
LOCUS       XP_077515442             130 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125695 [Amblyomma americanum].
ACCESSION   XP_077515442
VERSION     XP_077515442.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659316.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 12% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..130
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..130
                     /product="uncharacterized protein LOC144125695"
                     /calculated_mol_wt=14765
     CDS             1..130
                     /gene="LOC144125695"
                     /coded_by="XM_077659316.1:302..694"
                     /db_xref="GeneID:144125695"
ORIGIN      
        1 mehelsgirq smvriaaale rqvhltearq gsvlqpscft kgcagqegkl hlfvkirlnv
       61 qesfpirrqg snfckteyhr vtmglqasry stfedfiweq taiqlpqlps rmeyaltvpr
      121 lseetpcsdh
//
LOCUS       XP_077515550             197 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125752 [Amblyomma americanum].
ACCESSION   XP_077515550
VERSION     XP_077515550.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659424.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..197
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..197
                     /product="uncharacterized protein LOC144125752"
                     /calculated_mol_wt=21886
     Region          25..185
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..197
                     /gene="LOC144125752"
                     /coded_by="XM_077659424.1:1..594"
                     /db_xref="GeneID:144125752"
ORIGIN      
        1 mlptkeqile hlpkqfkqfs nvrvvdctki algqpnclqc alreyshykr gftakymvav
       61 tpagmtayis egyggresdk aifeqsglid apepiseaim adrgflidei cahylidlir
      121 ppfkkpkqql skgealrtqt iasarvhver aiqrmktlki latkvpwnmv gqlddilmii
      181 agitnisssi iaahlfl
//
LOCUS       XP_077515563             465 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125761 [Amblyomma americanum].
ACCESSION   XP_077515563
VERSION     XP_077515563.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659437.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..465
                     /product="uncharacterized protein LOC144125761"
                     /calculated_mol_wt=51738
     Region          <6..179
                     /region_name="RT_nLTR_like"
                     /note="RT_nLTR: Non-LTR (long terminal repeat)
                     retrotransposon and non-LTR retrovirus reverse
                     transcriptase (RT). This subfamily contains both non-LTR
                     retrotransposons and non-LTR retrovirus RTs. RTs catalyze
                     the conversion of single-stranded RNA into...; cd01650"
                     /db_xref="CDD:238827"
     Site            order(32..37,55..56,102,104..105,175..176)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238827"
     Site            order(32..37,55,104)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238827"
     Site            56
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238827"
     Region          <374..395
                     /region_name="zf-RVT"
                     /note="zinc-binding in reverse transcriptase; pfam13966"
                     /db_xref="CDD:433612"
     CDS             1..465
                     /gene="LOC144125761"
                     /coded_by="XM_077659437.1:1..1398"
                     /db_xref="GeneID:144125761"
ORIGIN      
        1 mggrlednlf vvtssieiaa kekrelilgf ldiaqaydsv ehnrlpisvs rglrqgcpms
       61 pilfmlfisg meqrlvesgv gfsltymedg atrtrrlpal lyaddivlla dntgdlqsll
      121 dicssqgdgl glrfnakksa avrfppease dsgqetpplh lqnnsvlwlp syrylgieis
      181 agrnyvrdfe kqlrtkairr raflssralw afnrfevtrs lwkavavpgl tfgnavicls
      241 svtrqgletr qrevgrmamg ahgsvpneav qgdlgwssfe areavaklsy errptssfqp
      301 ngrqkireqv rceeqrrwqq tarekpalav yqqgkskvek eplydntrgs gllcearsgv
      361 lrtrclraky tegmstmcel cggadetieh ivlecaglhp svsdaqdnss vgpmppdais
      421 gsnslgalam algfrrpdep pqwklvertk rrlehwwrtr hghap
//
LOCUS       XP_077515587             633 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125773 [Amblyomma americanum].
ACCESSION   XP_077515587
VERSION     XP_077515587.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659461.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..633
                     /product="uncharacterized protein LOC144125773"
                     /calculated_mol_wt=71379
     Region          100..165
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          383..435
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          464..623
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..633
                     /gene="LOC144125773"
                     /coded_by="XM_077659461.1:1..1902"
                     /db_xref="GeneID:144125773"
ORIGIN      
        1 maaegsgcls tlpqqkntre gtseefpada crelterlkr gqpllptrdv aalamvggyl
       61 hygrsaggac drvapdnpgl alsplarqwt qrerlicffv syhrfpkead tyrkwiaaik
      121 rdegplftvs katrvcslhf vesdfvanva nghrhlhqaa vpsvfpfkem kaarkppakr
      181 aplqnvrapr rkrrvvdvqp aqrkmhlsvn ssldedlvdm qnkqaayegt ddftiqenls
      241 hianrqresp gktvrssvct cnetiallee rlksskarel earaevqdlk lqlisknsev
      301 dllrrelvla qkniqkleea ppfgieqfkd nnddiefytg lpsynhflsl lnfldvgkhg
      361 eniiryeans cglrstenvg rphkitvenq iflvlvklrv gllhkhlghl fhisvstasr
      421 ifstwidymf lqltemtlwl srqavdaamp pafrerypst rvildateir cdvptsfvtq
      481 sqvysaykst htlkglvgis phgvltfvse lytgstsdre cvvqsgflnm pfdpddsvma
      541 dkgfkiadll eskniklnlp pfltrgqftp teveetqeia airihverki rkikgyhifd
      601 hlipitlvpl vnqmwtvcai ltnfqppllr dae
//
LOCUS       XP_077515626             134 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125791 [Amblyomma americanum].
ACCESSION   XP_077515626
VERSION     XP_077515626.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659500.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 35% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..134
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..134
                     /product="uncharacterized protein LOC144125791"
                     /calculated_mol_wt=14573
     CDS             1..134
                     /gene="LOC144125791"
                     /coded_by="XM_077659500.1:93..497"
                     /db_xref="GeneID:144125791"
ORIGIN      
        1 mageaeelrl lfvnktrrev dviclnyegr gvkcrspspn qlqawealts mvdlleeihv
       61 vrdmtsaasf dtivapaakv sfqeasywdi faptlvnvht ivtttplehv gvvvsvsemp
      121 stqcgklgqk patk
//
LOCUS       XP_077515669             306 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125823 [Amblyomma americanum].
ACCESSION   XP_077515669
VERSION     XP_077515669.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659543.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..306
                     /product="uncharacterized protein LOC144125823"
                     /calculated_mol_wt=34195
     Region          6..82
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..306
                     /gene="LOC144125823"
                     /coded_by="XM_077659543.1:72..992"
                     /db_xref="GeneID:144125823"
ORIGIN      
        1 mshmyccakw cstsgksgeh lfrfpsdhrf siwlkyanrl elletprekv yktyrlcskh
       61 ftrdcftsda ctrltheavp svkvheprlr alvhpdfyee gaaqgiesas plqstaapve
      121 vmlghdylpl pstlglgtia gadsavhdtd dphssrapql tdvqttpppp adeegaaqst
      181 pirviasasw dspdyaeagp sglsgakesv rqpvmtpasg tpksrrrkga vvytprtkkr
      241 feklhsrsdr yrkalsrirk rhdtrrvtqq ealnklephl pqdlfelvka qirlcsfpks
      301 kkhwsk
//
LOCUS       XP_077515688             106 aa            linear   INV 25-NOV-2025
DEFINITION  importin-7-like [Amblyomma americanum].
ACCESSION   XP_077515688
VERSION     XP_077515688.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659562.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..106
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..106
                     /product="importin-7-like"
                     /calculated_mol_wt=11395
     CDS             1..106
                     /gene="LOC144125837"
                     /coded_by="XM_077659562.1:1..321"
                     /db_xref="GeneID:144125837"
ORIGIN      
        1 mrpptspsta qrgwhgtlsp epsasfrdda vdsvkpeqgm eptttpldee ecpvdeylvs
       61 kevmrgiqnt dpmwfgalts gpqsvkqkrs pleanvlagq rnaaae
//
LOCUS       XP_077515711             658 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125853 [Amblyomma americanum].
ACCESSION   XP_077515711
VERSION     XP_077515711.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659585.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..658
                     /product="uncharacterized protein LOC144125853"
                     /calculated_mol_wt=74536
     Region          <191..649
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..658
                     /gene="LOC144125853"
                     /coded_by="XM_077659585.1:24..2000"
                     /db_xref="GeneID:144125853"
ORIGIN      
        1 mavtvvaffy lfrappshrs rqlicitddc rlhalfldyn inntfspcad fdghicstwt
       61 psqtytqmga talgqvavnw vdnflpllrr aadpikiaek plevyeacvq eredataada
      121 ahfaaflkev nlvwpersgt nalgllinva fkwqmtfwls frfrmvsqkh mkffivagdp
      181 dvlllfamnh ryvmqrgsyv eywmshyttl ygnksvppas qiktsareqt dilrvltdvr
      241 kkkrslvtvv pvgntanfng rvtlfdwleq lkthlpqeqf ppsgdqvlgd stlleaigll
      301 fnkysdeqlv yhlswqfiql yfltlertpl qivhwgktys sayvpvyctw fveevfrpll
      361 salhvkmaia pgdttrlnfa lgglmeklvs tvnssqlesg pkraamaryq smhiqiwppg
      421 kyfndeetek ayscyptkrd sfvqywiesh rclqrrvgta fhrestgmhr llssspvtyd
      481 pitnvlavaa salahplyyp hgtpamfygg lgflivaeal kaldraslav lsnastplsn
      541 sttgiikepr cftvqendtr atyfaalnia hsafvedean eaqerrrpis rehseenvff
      601 lticrmmcra rtlqnagmin cnalfkscpy fanafacppe splnpvercf sfqanrnd
//
LOCUS       XP_077515746             577 aa            linear   INV 25-NOV-2025
DEFINITION  serine/threonine-protein kinase PLK1-like [Amblyomma americanum].
ACCESSION   XP_077515746
VERSION     XP_077515746.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659620.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..577
                     /product="serine/threonine-protein kinase PLK1-like"
                     /calculated_mol_wt=65265
     Region          28..282
                     /region_name="STKc_PLK"
                     /note="Catalytic domain of the Serine/Threonine Kinases,
                     Polo-like kinases; cd14099"
                     /db_xref="CDD:271001"
     Site            order(36..40,44,57,59,91,107..110,114,116,153,155,
                     157..158,160,171,174,190..193)
                     /site_type="active"
                     /db_xref="CDD:271001"
     Site            order(36..40,44,57,59,91,108..110,160,171)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271001"
     Site            order(40,114,116,153,155,157,174,190..193)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271001"
     Site            order(170..178,183..193)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271001"
     Region          390..477
                     /region_name="POLO_box_1"
                     /note="First polo-box domain (PBD) of polo-like kinases
                     Plk1, Plk2, Plk3, and Plk5; cd13118"
                     /db_xref="CDD:240561"
     Site            order(396..399,468,470..474)
                     /site_type="other"
                     /note="phosphopeptide binding site [polypeptide binding]"
                     /db_xref="CDD:240561"
     Region          494..572
                     /region_name="POLO_box_2"
                     /note="Second polo-box domain (PBD) of polo-like kinases
                     Plk1, Plk2, Plk3, and Plk5; cd13117"
                     /db_xref="CDD:240560"
     Site            order(499,518,521,523)
                     /site_type="other"
                     /note="phosphopeptide binding site [polypeptide binding]"
                     /db_xref="CDD:240560"
     CDS             1..577
                     /gene="LOC144125869"
                     /coded_by="XM_077659620.1:1..1734"
                     /db_xref="GeneID:144125869"
ORIGIN      
        1 masgflgsst anvnrpvpkd vidreanrky llgkflgkgg fakcyelvde atntmfagkv
       61 itkdrlvkny hkemlaqevr ihrslthkhi vgfkgytede knvyiimelc trrslmemlr
      121 rrktltetea ryilhqllla crymvqkrvi hrdlklanll lnedmelkvc dfgmatridh
      181 vgerkknlcg tpnfmapeil sntghsfevd vwsigcilyt llvgsppfat ssmeetcyri
      241 qknvfhmpst lsstasalir rilqieperr psiediledd fltygplpsl lptsclvtep
      301 rfdslnaslp vggrrvvler neasgctaas pgaaavsaqp avsprpapkg sqqemhlkel
      361 erlldrlcsk rpperhfesp detedpasap vfwiskwvdh tnkyglayel cdnsvgvlfn
      421 ddtrimrlnn yvsvryvdee wlehnhtlde yppslhkkmt llkhfcgymk ekllaagkav
      481 rpregvslvr lpclgtwlrt rmaisfyltn gtvqvnflhd htkiilcplm aavsyidsdq
      541 afhtyrlatl drgcppeifs rlkyarsiva rlagaps
//
LOCUS       XP_077515760             247 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125877 [Amblyomma americanum].
ACCESSION   XP_077515760
VERSION     XP_077515760.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659634.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 42% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..247
                     /product="uncharacterized protein LOC144125877"
                     /calculated_mol_wt=26871
     Region          118..198
                     /region_name="retropepsin_like"
                     /note="Retropepsins; pepsin-like aspartate proteases;
                     cd00303"
                     /db_xref="CDD:133136"
     Site            order(132,134,136,157..159,196)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133136"
     Site            132..134
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133136"
     Site            132
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133136"
     Site            order(157..160,164..167)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133136"
     CDS             1..247
                     /gene="LOC144125877"
                     /coded_by="XM_077659634.1:1..744"
                     /db_xref="GeneID:144125877"
ORIGIN      
        1 mnlkevveer rvktlsqrld ladqfveaqg gtnltklkni nseiagktds rdarfylkgs
       61 tkvlpvqkvs cvrassddhl eeqddhgfve lkggekipvv navmrpppdr faegtpvlpg
      121 kvagravsvl cdtgastviv krdlvqdeel tgtksavfvv dgtirflpea kvtvdtpyys
      181 gevtalcmkd plydlilgni qgvreaenpe sltkikgqpv pakskrhere eatvaavtre
      241 eskdqgk
//
LOCUS       XP_077515803             411 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125886 [Amblyomma americanum].
ACCESSION   XP_077515803
VERSION     XP_077515803.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659677.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..411
                     /product="uncharacterized protein LOC144125886"
                     /calculated_mol_wt=45510
     Region          71..138
                     /region_name="BEN"
                     /note="BEN domain; pfam10523"
                     /db_xref="CDD:463133"
     CDS             1..411
                     /gene="LOC144125886"
                     /coded_by="XM_077659677.1:1..1236"
                     /db_xref="GeneID:144125886"
ORIGIN      
        1 mthilvkwis elkwdvypvr sltdvkigmr llmetntisg lrgrtniggg vmvektilsr
       61 lhahcrtapa kfarnllkal ftldelrgms lygkgtnsrk agqvkealdp vcvkavigey
      121 atvcreshdh awrtlspegr ggqssqsrfk atqagkrgkl frphlcltts vraglqawaw
      181 nlrravsask klrrvavgts ppssgtsssl ppgsrlfiir wnpqlgrtst gplllgqhrg
      241 dgtahrievr irrsdqrspq isrhrsgrlm qqhghvtrrs aarsqllrrf myaiaivlsv
      301 vlplpvkeas tgsrmkvgnr mwgmrqflhn rswfnlraap fddyqlgapr atagsrpvae
      361 fprtsldlnr tiwaatgtad gfilanigts eparrhsrma sepaaarrrv s
//
LOCUS       XP_077515861             340 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125919 [Amblyomma americanum].
ACCESSION   XP_077515861
VERSION     XP_077515861.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659735.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..340
                     /product="uncharacterized protein LOC144125919"
                     /calculated_mol_wt=37683
     Region          <118..>318
                     /region_name="COG4372"
                     /note="Uncharacterized protein, contains DUF3084 domain
                     [Function unknown]"
                     /db_xref="CDD:443500"
     Region          118..>211
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     CDS             1..340
                     /gene="LOC144125919"
                     /coded_by="XM_077659735.1:1..1023"
                     /db_xref="GeneID:144125919"
ORIGIN      
        1 mqplatpple nwtkqaasaa pssptapfas aikqrhppsa hcglcgcatv rtanpltfvm
       61 qvgvpytlfa kkssnyclvq lpspqccfcl tvecvnvira llllsgdvet npgppntdav
      121 laelqklsag qrklisevqg lknqlvttdk tisalseria nietqfevis tlrteietlq
      181 tstartthql seidtrldyl enrsrrnnlv fyglpdpnek esydqsekii qhcldslnlv
      241 idperahrlg rhsidrqrpi ivklsafktk gvilsngpkl kntdfsiged fsrpvrnark
      301 hlvafartks gpfslryktl hmgpkrymfd qdtqtvkeia
//
LOCUS       XP_077515875             168 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125926 [Amblyomma americanum].
ACCESSION   XP_077515875
VERSION     XP_077515875.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659749.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..168
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..168
                     /product="uncharacterized protein LOC144125926"
                     /calculated_mol_wt=18275
     Region          <54..>136
                     /region_name="P2RX7_C"
                     /note="P2X purinoreceptor 7 intracellular domain;
                     pfam20478"
                     /db_xref="CDD:466627"
     CDS             1..168
                     /gene="LOC144125926"
                     /coded_by="XM_077659749.1:1..507"
                     /db_xref="GeneID:144125926"
ORIGIN      
        1 mprcppyenv tsvgsdclll vasqysqgrs grhidvvcdf amdpelekrl reatavmgle
       61 pyaftpfrdp qiddisdspa gspdspcgre ndsvpwftcg ccesmptvde qlccrsvpqv
      121 vneqgdgcit ghpafesvcc tpqvlravyi alaeenvdnt rhdddvnk
//
LOCUS       XP_077515898             160 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125940 [Amblyomma americanum].
ACCESSION   XP_077515898
VERSION     XP_077515898.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659772.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..160
                     /product="uncharacterized protein LOC144125940"
                     /calculated_mol_wt=16462
     CDS             1..160
                     /gene="LOC144125940"
                     /coded_by="XM_077659772.1:459..941"
                     /db_xref="GeneID:144125940"
ORIGIN      
        1 mlgqpkqksk eedarkkrdr hktgggsaqc tvsaqseqvi avashimtrv gnqtdsdggi
       61 dlppvaslpv irllqpmvdg agneefryae ddwepplvde pqspaaavgk sgatpeaieq
      121 esnlffpavq padasstaaa gcssataasg aalvplllkq
//
LOCUS       XP_077515935             425 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144125962 [Amblyomma americanum].
ACCESSION   XP_077515935
VERSION     XP_077515935.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659809.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..425
                     /product="uncharacterized protein LOC144125962"
                     /calculated_mol_wt=48362
     Region          113..201
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     Region          368..383
                     /region_name="zf-CCHC"
                     /note="Zinc knuckle; pfam00098"
                     /db_xref="CDD:395050"
     CDS             1..425
                     /gene="LOC144125962"
                     /coded_by="XM_077659809.1:1..1278"
                     /db_xref="GeneID:144125962"
ORIGIN      
        1 mrermhqaeg dppvitdltp vhqrrsrrlr gespefgplp aatrttqprt seepaamste
       61 srlpahilcq qprtppsfhg dvfedaedwl etfervaryn gwsderklhn vyfaleesar
      121 twfenheasf psweafcrnl latfpnadrr eqaeaalhsr nqrpnesvcm ffedmtrlfk
      181 raypsmsedk klrhlmrgvk qelfaglvrs pprtvaefls eaatmektlq qrarqynrdv
      241 nafgpdtcsm hlgcapddlr elirsvvree lqalrtpsnm psfasltdvv rdevrlaaqq
      301 qasevplqld arptyasvlr qaptsgntin aaaplvrpps psrdpalpay ertrksqvwr
      361 apdrrplcyh cgeaghlyra cphrrmglrr fapddpcprn gerpleiash llerqnlvts
      421 vaaah
//
LOCUS       XP_077516028             160 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126022 [Amblyomma americanum].
ACCESSION   XP_077516028
VERSION     XP_077516028.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077659902.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..160
                     /product="uncharacterized protein LOC144126022"
                     /calculated_mol_wt=18040
     Region          38..>108
                     /region_name="SWIM"
                     /note="Uncharacterized protein, contains SWIM-type Zn
                     finger domain [Function unknown]; COG4715"
                     /db_xref="CDD:443750"
     CDS             1..160
                     /gene="LOC144126022"
                     /coded_by="XM_077659902.1:149..631"
                     /db_xref="GeneID:144126022"
ORIGIN      
        1 mirlqcgfkk icqldsffrk anlikgekll nhvysveqii ennearlkgk cvsqvsdsvv
       61 ydvclelaws chekqellvr egrctckagi aakckhaaav clyvdqhevk sctsepqkwg
      121 kpkgnpkrsl elflfphgmk nvafeslark hmpyaqnevt
//
LOCUS       XP_077516135             429 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126083 [Amblyomma americanum].
ACCESSION   XP_077516135
VERSION     XP_077516135.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660009.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 27% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..429
                     /product="uncharacterized protein LOC144126083"
                     /calculated_mol_wt=45469
     Region          <2..160
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     CDS             1..429
                     /gene="LOC144126083"
                     /coded_by="XM_077660009.1:1..1290"
                     /db_xref="GeneID:144126083"
ORIGIN      
        1 mryltpsfis ilglgaitsa vmssadssvl sasslitnni yysilrpras vreistvvrv
       61 mvclvgvatt vmalsvqsvy tlwslcadav yvvlfpqlvc lfymdhvtnt yglicgvstg
      121 lvirllcgep amhvpvvlkl plydedagqk fphrtlcmll nlgatlvgsl fwervfrlgy
      181 vpksfdycrc faetcdephv aaaaaldtkp fensradasq ldvsrlersh agvsragasr
      241 sgwkpadtta gdadrtegsq lffpessrmh srapnfpvgs qtalpatkaa srlsthtksq
      301 pggasvhstk ssqsskstts sepshkrpes diasakssds gaakdapsvr saageherqt
      361 agadskvprs kprvnatkme aaapntdgaa pveetthvkg lpkpcpskas rkikasrssi
      421 slgsqkkal
//
LOCUS       XP_077516145             274 aa            linear   INV 25-NOV-2025
DEFINITION  high-affinity choline transporter 1-like [Amblyomma americanum].
ACCESSION   XP_077516145
VERSION     XP_077516145.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660019.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 38% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..274
                     /product="high-affinity choline transporter 1-like"
                     /calculated_mol_wt=29853
     Region          9..>266
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     CDS             1..274
                     /gene="LOC144126089"
                     /coded_by="XM_077660019.1:1..825"
                     /db_xref="GeneID:144126089"
ORIGIN      
        1 malntfaalc vtmyyvgitt lgvwsgrklh igqrtgrrgs rlssraqdda dsfllryflc
       61 drrmplllgf ssmtatwvgg gylngtaeav yrfglircqa pfgyaislil ggayfvsklr
      121 qtdaltmldp fqrrygkwvs llllppavcg elfwtaavls algstveviv qldarmaivv
      181 saavtffyts lgglysvtht dsfqaagiti glwacvpfcl gnkavglapy pdnewrgeih
      241 madigqivde fattvlggip wqvrvflllf ipsm
//
LOCUS       XP_077516182             352 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126110 [Amblyomma americanum].
ACCESSION   XP_077516182
VERSION     XP_077516182.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660056.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 35% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..352
                     /product="uncharacterized protein LOC144126110"
                     /calculated_mol_wt=38561
     CDS             1..352
                     /gene="LOC144126110"
                     /coded_by="XM_077660056.1:165..1223"
                     /db_xref="GeneID:144126110"
ORIGIN      
        1 mappgrsrsp ttpttppfvi mapprdpgtf sgtdgsdied wislyervsn ynrwdptlml
       61 anvifylngt arvwyethee eltswntfkq klktlfgrpd drqlaakkql atraqsstes
      121 yvayiqdila lchkvdaats etdkvghvlk giaddafhll vckdctsids iinecrrfeq
      181 vkgrrispsf arlpnmaats scedhrspsr tpsdapavtd svtrivrrel qaqapvispp
      241 svpdttlpav sliqavvhvg hgsyvpvaaw nrllalgses tfckqlavcl wgnetlaqrs
      301 ltdrlgqitw ltlgnktfam tayvnapdds ckgainnvpt nntptggirr sn
//
LOCUS       XP_077516208             561 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126120 [Amblyomma americanum].
ACCESSION   XP_077516208
VERSION     XP_077516208.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660082.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..561
                     /product="uncharacterized protein LOC144126120"
                     /calculated_mol_wt=60508
     Region          <61..181
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            67
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238827"
     Region          286..413
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     Site            order(290..293,322..324,327,352,391,409)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260008"
     Region          <472..508
                     /region_name="zf-RVT"
                     /note="zinc-binding in reverse transcriptase; pfam13966"
                     /db_xref="CDD:433612"
     CDS             1..561
                     /gene="LOC144126120"
                     /coded_by="XM_077660082.1:1..1686"
                     /db_xref="GeneID:144126120"
ORIGIN      
        1 mslcaasncg lleaashsvi healsrlgvt gpllafiqaf lqgrtfrvrv ggqlssprpv
       61 aagvpqgsvl spflfnlama glqaslksrs shrvrcsvya ddvalwvrgt prrirsmrts
      121 lqralnaaqa ylssigltvs pakteallyh prgrrahitt lrlgadgltw rnqvkylgll
      181 idrrltwlpa vgallprlrr idqavqrlqa rdkgcspswa lrlyhaaats lvtyalplap
      241 fpppptrppl pisvdirscs rrrtpvaalq qtaatllqes lgghlqvytd gsvlpatgqa
      301 aeacvapalg aalschlrfp anstaaelag lhlaadllll lpgqqpavil tdsraalqll
      361 rqhqprqhtv anlkarlmai qdagrpvslq wlplhvgitg neaadqlaga ahtngspvss
      421 kvtqldfarp alrqavralh pdprvasgvr fmrvpdclpr reralilrlr tgcswtqarl
      481 hrhgrapspa csfcgadetl ghllcacpnl eaarrdmtag yrnlglpsys dqdllhpkrs
      541 qteafrllle flqstgfatr l
//
LOCUS       XP_077516220            1189 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126127 [Amblyomma americanum].
ACCESSION   XP_077516220
VERSION     XP_077516220.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660094.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 21% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1189
                     /product="uncharacterized protein LOC144126127"
                     /calculated_mol_wt=132633
     Region          4..93
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          <356..546
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..1189
                     /gene="LOC144126127"
                     /coded_by="XM_077660094.1:19..3588"
                     /db_xref="GeneID:144126127"
ORIGIN      
        1 mptcfvpgct sgyrrntekr hffspptdpn llakwsraip radkvlgktc kvcdahfqeg
       61 dilksykhvi ngevieierg vwslrsdaip tifpnlplyf ssnrkrrksp kkratvsrda
      121 vatqsdaaed idiereqpep vqdsclhvne lveksaslrl prqwrsaale dengrrsqiv
      181 fyragleadv ytalksvvvr edmsyvvsar rklvptlpmd laitsladve aivqvvqtmq
      241 achgctssrg gtvyhkqcrv lteaekgvci kcakmtkdlk rrsglkpkkk vclaapvlrk
      301 rliraaaawe qkkkeisqls eklkqafsts lgevtqdlpe aqqmviqaai mqqaaksprg
      361 hryttdwlmi clllrltspk cyrtlsdmki lplpsanrlv qllkglpcgy glnkfalgsi
      421 klhmagkpeh ytygcliide vklregteyn rssykfngfv dygditkvgt dqladhalvv
      481 mfnpmfaswv qpiatfaakg aapgwvlakv vmssvlqlhe hgarvlavis dgagnnksmw
      541 thlgisgkve hpqckiehpc lpegtllhfi sdvphivkci rnhmmkhqyg qvgeyqanfq
      601 hyerlfeaek kaaikvvpkl tehhvkpqkl qtmtvrlatq lfsrsvsigl kvyrqmmvpg
      661 fansfgteqf tmllndlfdi lnskvpaagi rkgspkikfl ddflamlnet esipnvklfa
      721 srqtieslrv tlmsvlslve flfnqgvsyi ltaslnqdpl erffglvrsf ggdedhptvt
      781 kfsqlfrlls lytpvklatk gnceagderv llstfeslge krreallqkr kvkdevfqrl
      841 tslpsrglsa pfdddvysvs spekaalyyl agyvahkmmk viscmecksd tmasqdslpp
      901 eamlvieref vtgslvcpsr klfacvksie etkkeafgdl fwnpqaatlm gvvregvqqa
      961 lgtpsatqpq amtnaalirp pppqrhplrh rrdeplapgc hlpasarqpy errsilgnat
     1021 pgelpstdrc afiveevtvp pgssviltvg ttkainaeai ieenmqmlld rgfiiarria
     1081 hfsngkaesr slqefdvtvi yksgrkrsya drlsrapvda tppdddedaf lvsispksfa
     1141 qqqhsdldlk hlieyledat clsgvlfcrt fplftvsgpr qswlgqawt
//
LOCUS       XP_077516273             154 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126163 [Amblyomma americanum].
ACCESSION   XP_077516273
VERSION     XP_077516273.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660147.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 16% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..154
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..154
                     /product="uncharacterized protein LOC144126163"
                     /calculated_mol_wt=16560
     CDS             1..154
                     /gene="LOC144126163"
                     /coded_by="XM_077660147.1:567..1031"
                     /db_xref="GeneID:144126163"
ORIGIN      
        1 mpailrarns shardpthrp pfaaltsvlc cqrpasigpc vlpafgcvil pdpfmlktks
       61 cvevvqatkl rvicatdleq agkladgqwy slskasffsk akttflgswa mylgtcgqdn
      121 agqhasarsk kkhktgttlp tyqsqgmqpd trec
//
LOCUS       XP_077516286             338 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126168 [Amblyomma americanum].
ACCESSION   XP_077516286
VERSION     XP_077516286.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660160.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 14% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..338
                     /product="uncharacterized protein LOC144126168"
                     /calculated_mol_wt=37506
     CDS             1..338
                     /gene="LOC144126168"
                     /coded_by="XM_077660160.1:1..1017"
                     /db_xref="GeneID:144126168"
ORIGIN      
        1 msllmypgkl yneaaqalvs rypnlvdasg tgydswreal rfkakyerrk iriqqndgad
       61 sppskkptkd gkvatvggka lqrvsrpsvp gvqcevgsfc lqatapdged eesiaghieg
      121 mksavqkarp nmvyiidcmr rtfptrrkwi gsedpsveva iqkfpalams siaqlefeli
      181 tsvpvlerle eavhrteeki vhaarkkrhl egfledfdar idgtseaavy ettvtaavcl
      241 lpsmvkerve tfvrpfdpaa vhyiptvvhr ggilttsdfs vclekicike tsllaavatq
      301 malywafniv fdkkaqrsfd llcrlinvds glrptplw
//
LOCUS       XP_077516297             482 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126178 [Amblyomma americanum].
ACCESSION   XP_077516297
VERSION     XP_077516297.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660171.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..482
                     /product="uncharacterized protein LOC144126178"
                     /calculated_mol_wt=54642
     CDS             1..482
                     /gene="LOC144126178"
                     /coded_by="XM_077660171.1:1..1449"
                     /db_xref="GeneID:144126178"
ORIGIN      
        1 mefeqrisnw lpaldkeive ltvqklqkcg veflehlkyv veedlqflmp isrrillerf
       61 hsaatpnppv slspndiqsp tstqcasfst pwevfppqlm kacqegkrpv ksdlnemvrl
      121 vsdhilainr kpgrkilrai aaeivshypk tfedtlngav igsgietllw klencvnnkr
      181 rpsseqphqf epddeldlsv krvkiqrdsy gcvawqpklp sdetadsqek kkddllsqfr
      241 lafpdetmva qlmsetyasq rqlinaskni rdierswpfl fqakhltnhv nillgsnvak
      301 tfddrlktvg rqvfryahsq vkkecvkscl qeieaaktnr ksmaveyeam pllllaifge
      361 dkelfyklte emasdddlgd lpscpficvk gwtfltansy ticvdthpvl haskpdeafk
      421 llffahfafn iqyqketslc leftqraiag inpargtkvq knggkqhcls prvaalvtal
      481 kd
//
LOCUS       XP_077516333             195 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126200 [Amblyomma americanum].
ACCESSION   XP_077516333
VERSION     XP_077516333.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660207.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..195
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..195
                     /product="uncharacterized protein LOC144126200"
                     /calculated_mol_wt=20940
     CDS             1..195
                     /gene="LOC144126200"
                     /coded_by="XM_077660207.1:1..588"
                     /db_xref="GeneID:144126200"
ORIGIN      
        1 marreeiadn csasfecdvs cnvafphrgg llrhheqvhg fvpgrirlef ssltasdcga
       61 stpgrstcse aldsdleqkp egtsavlgqg aavpyfvepf spvthgwngm tsvdksgsaa
      121 invsklepdt dfidsgppif eqrescdhve iylnsrrigy vgfvagtlad mhltlllhmq
      181 igsgcpiiir kllhv
//
LOCUS       XP_077516346             286 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126207 [Amblyomma americanum].
ACCESSION   XP_077516346
VERSION     XP_077516346.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660220.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 11% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..286
                     /product="uncharacterized protein LOC144126207"
                     /calculated_mol_wt=30736
     Region          189..277
                     /region_name="Spaetzle"
                     /note="pfam16077"
                     /db_xref="CDD:465007"
     CDS             1..286
                     /gene="LOC144126207"
                     /coded_by="XM_077660220.1:1..861"
                     /db_xref="GeneID:144126207"
ORIGIN      
        1 mrlpallgav egvqhalyad ditlwatspt qvvfhdelia sgapsrsstg tlqaqdfgta
       61 ttapatptht paaavttaea fcvrgghasn tprenlltgt attepavvdf epsyaagepf
      121 theppehpqs yassssstta aeplfsllds nsidgasplg qsrpasqehq degdqaplpg
      181 pqplrrgvna cpvkqevlap ywanntrgkv lallnvypfe qyvhaekcaf eneqmfcrdg
      241 crceqqfrlh rllafdprne crgifadwfr fpsccvcicy dvgspp
//
LOCUS       XP_077516362             366 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126209 [Amblyomma americanum].
ACCESSION   XP_077516362
VERSION     XP_077516362.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660236.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..366
                     /product="uncharacterized protein LOC144126209"
                     /calculated_mol_wt=41440
     Region          224..310
                     /region_name="RT_like"
                     /note="Reverse transcriptase (RT, RNA-dependent DNA
                     polymerase)_like family. An RT gene is usually indicative
                     of a mobile element such as a retrotransposon or
                     retrovirus. RTs occur in a variety of mobile elements,
                     including retrotransposons, retroviruses, group...;
                     cd00304"
                     /db_xref="CDD:238185"
     Site            235
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     CDS             1..366
                     /gene="LOC144126209"
                     /coded_by="XM_077660236.1:1..1101"
                     /db_xref="GeneID:144126209"
ORIGIN      
        1 mkveggrrde artrvvsila krptrqashm lpeernairs lrrnreivil padkanatvi
       61 lnstdydkkm mellkrqehh kalyyrilch ngsapalygl pkihkegvpm rprvdftrsp
      121 lcqlsgylhr vfaplvgkgp tfirnsfdft ekvrdavved ddiivsfdvt slftsvpvdl
      181 avdvcaaalq ndeklsertp leaqdllrll kfclkntyfv frgsfykqvh gtamgasisv
      241 taanltmeai enralasfhp rpnvflwyid dcfcivkkda laaftrhins mekaiqftve
      301 eewfdklshn gwtlsqlqyp sgfhlcvmlp hvaenvtnqf vsdlnecnvt scgrafrlss
      361 sgrkke
//
LOCUS       XP_077516378             143 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126212 [Amblyomma americanum].
ACCESSION   XP_077516378
VERSION     XP_077516378.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660252.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 26% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..143
                     /product="uncharacterized protein LOC144126212"
                     /calculated_mol_wt=15989
     CDS             1..143
                     /gene="LOC144126212"
                     /coded_by="XM_077660252.1:1..432"
                     /db_xref="GeneID:144126212"
ORIGIN      
        1 mdpdsaddpq dvimkeclrd lkktgdkwtg ylqsheelqa fekklaavhv qfsttssrnh
       61 kcgasqlmfs snrgtvpvsl dmpfqvvrqv dkdcvlgidh hkdkcestst aqdptdwsse
      121 vfckkkrssc keprrrgalq aci
//
LOCUS       XP_077516494             434 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126275 [Amblyomma americanum].
ACCESSION   XP_077516494
VERSION     XP_077516494.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660368.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 42% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..434
                     /product="uncharacterized protein LOC144126275"
                     /calculated_mol_wt=47661
     Region          <14..95
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            19
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238827"
     Region          <229..286
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..434
                     /gene="LOC144126275"
                     /coded_by="XM_077660368.1:1..1305"
                     /db_xref="GeneID:144126275"
ORIGIN      
        1 mvgdtqgarq pnrtgvpqga vispalfnia msglplilts ipglnfavya dditlwtnsg
       61 spaeqelalq tglnavhdyi kkcgmktspe kteyvaving prlraeaern lislhlgdsv
      121 tcrkqtiril gmlidedgga kswiyntvls chqalhlmrr vsargrgike vglcqialal
      181 itskilyays ymrlnatqrl qlerinrkam rlvtglpqfc pvedpapcty hvytdslaay
      241 tacraggady avlsairtea rrlreegyti virwipaheg itgnekahqa araaalyapl
      301 pgctlvsasw arddvahpss ppmdraetwh raqcyrrril qhvanpeqly hrpprtfrrv
      361 eavllrraqt gqllspakln linphsyptp qcpacpasaa lerrvsdadv arinfhipsf
      421 fcgtrdgmva grpg
//
LOCUS       XP_077516512             134 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126289 [Amblyomma americanum].
ACCESSION   XP_077516512
VERSION     XP_077516512.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660386.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..134
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..134
                     /product="uncharacterized protein LOC144126289"
                     /calculated_mol_wt=15173
     CDS             1..134
                     /gene="LOC144126289"
                     /coded_by="XM_077660386.1:1..405"
                     /db_xref="GeneID:144126289"
ORIGIN      
        1 mspemfdtll sfvtddltrq yvmreplepg ewlaitlsyl atgqdikdig lacrvgieka
       61 rvtihfccri iwarlkdqfm kvpsagdwve isegfkkqwq fpnclgavdg khvaimapan
      121 tgsayfnykt fwmk
//
LOCUS       XP_077516531             618 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126326 [Amblyomma americanum].
ACCESSION   XP_077516531
VERSION     XP_077516531.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660405.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..618
                     /product="uncharacterized protein LOC144126326"
                     /calculated_mol_wt=67614
     Region          <349..595
                     /region_name="CYCLIN_SF"
                     /note="Cyclin box fold superfamily; cl40454"
                     /db_xref="CDD:477360"
     CDS             1..618
                     /gene="LOC144126326"
                     /coded_by="XM_077660405.1:1..1857"
                     /db_xref="GeneID:144126326"
ORIGIN      
        1 myhaggggvp gvlrslshgp rrgrsscpeg ilveqgwwqp iqespistea pvdvssiqnl
       61 rltpttdhgr valhesgept tgpsgtfpar tnasvtgner drkqagnsll rmavqgggda
      121 ppsfnaalli smlgrgrgrg waisdagpla lgssqprqgh rlsahassal slqqdrrhsa
      181 tgtssvvper shtsksherp rhqaqvpqhs lvkaansivr paiqdvrqlp wprpvspvap
      241 agqeapppem gpakerrpts vqkltkgdgs appsfpankc tlgrhkgrgr irgaaplvsv
      301 rsaqpylgrl vsskppsavs sqqrstqdcd etasrtlfsd pkeeflrvls erssatnaap
      361 qpessstsrv pdsdaddyif lqetkwpikp aflmaqkevd rsirqhkvqw lidtrnalqf
      421 spetlflavs ladrylegcv vdkrqmellw aaalcvavkh kepeyhvvyr syvasakrmg
      481 esreqelcnm eedvsravgw sldlalpldf lrrnckaaea skqehltamy ilevclvdyi
      541 mawmppskka asalycamhy qgrknpcwsq kmqlcsghai gslqpivtrm qelliaaces
      601 diwtpyeiye kkwshtlg
//
LOCUS       XP_077516533             277 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126335 [Amblyomma americanum].
ACCESSION   XP_077516533
VERSION     XP_077516533.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660407.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 16% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..277
                     /product="uncharacterized protein LOC144126335"
                     /calculated_mol_wt=29361
     CDS             1..277
                     /gene="LOC144126335"
                     /coded_by="XM_077660407.1:51..884"
                     /db_xref="GeneID:144126335"
ORIGIN      
        1 mhhagggdvp gvlrslshah ergctssseg lsdkqgrpqp iqespisaaa qadvssiqnl
       61 rlsptidhgr valhesgept tgpsgtfpar tnasvsgner drkqagnslr rmavqgggnt
      121 psfyaalrml grgrgrgqti sdagpltlgs sqprqghlls ehassavssq qnlpqsscih
      181 dgrlavkdrr hsatgtsrvv pershtresn erpqrqvqvp lhslvkaans igrpavqvnf
      241 fqlkreaesd hptdvgrwqa aaepeslprs pnnkkpg
//
LOCUS       XP_077516538             670 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126344 [Amblyomma americanum].
ACCESSION   XP_077516538
VERSION     XP_077516538.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660412.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..670
                     /product="uncharacterized protein LOC144126344"
                     /calculated_mol_wt=75163
     Region          16..94
                     /region_name="DUF4363"
                     /note="Domain of unknown function (DUF4363); pfam14276"
                     /db_xref="CDD:464121"
     Region          132..267
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     Region          322..>436
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     CDS             1..670
                     /gene="LOC144126344"
                     /coded_by="XM_077660412.1:1..2013"
                     /db_xref="GeneID:144126344"
ORIGIN      
        1 mdrlkeerta rreqgtrlis eakaavetad wktiteliek lesnhdelvk inneyepyis
       61 eedfaaeyea svkyeeaake mlgqlkaqea vlrnakcpps dspavpvrsg htegtalqri
      121 plnlpalqlq pfsgelctwp sfwekfkvlv hdndaltkee kfqylksllt gaaaesidgf
      181 qatgdcydna idiltrrygg llpifqehma rlrstpsves sqdvrglrnl fdhvqrhiga
      241 lqalnvpsva ysamtadvly ralpadiiig yhrrialsnp pttplalptc vgsgqemtvv
      301 piapaalapd ryvqdfltyl rievesrers girdmrriye mqgmqtrpps leheysilgt
      361 ppkptdmccf fcgsqwhave dcdialtkeq klqrlardsr cfrctmrghl akqcprtvtc
      421 kwcngrhias mcdrnrkpap ilpvaplppa mhmgshlclh tevllqtfrl wivtdqrcay
      481 irglmdggsq rtfiredvsr tlnlreviht nlrlhtfght gsvlehrriv evplrsqydc
      541 tvyrinaiev pfvcdeipqv ppesffvkvl esrgqkaadk llqqnmpavp gmslligadn
      601 lwkfltgalg rmsgnaaede hlvlvcdkll rrpvdsatlp qalgfsfplp yegaqvalpr
      661 psswlppsdg
//
LOCUS       XP_077516558             263 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126376 [Amblyomma americanum].
ACCESSION   XP_077516558
VERSION     XP_077516558.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660432.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 34% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..263
                     /product="uncharacterized protein LOC144126376"
                     /calculated_mol_wt=28190
     CDS             1..263
                     /gene="LOC144126376"
                     /coded_by="XM_077660432.1:1..792"
                     /db_xref="GeneID:144126376"
ORIGIN      
        1 mlafvryedr mkaivpvali krfsptnegd fnsekkheiy wcsedgvvlg yypayvlala
       61 gaqlpevmlt pttsatgatg aqlpeamlta ttsatgatap pstspeevav pcedttqakt
      121 patlslntld avngqvqvll vlsiteakwn fllqnrsdak ftiemalvvw srqeaasrsl
      181 tgeacrsmag slrkmpatpe kveavanclq kyvelhpaae ppvhsrvgaa rkylrsffte
      241 agrqgkrgka vktksaaevl ena
//
LOCUS       XP_077516562             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126401 [Amblyomma americanum].
ACCESSION   XP_077516562
VERSION     XP_077516562.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660436.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 12% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144126401"
                     /calculated_mol_wt=14107
     CDS             1..133
                     /gene="LOC144126401"
                     /coded_by="XM_077660436.1:431..832"
                     /db_xref="GeneID:144126401"
ORIGIN      
        1 mlgqpkqksk eedarkkrdr hktgggsaqc tvsaqseqvi avashimtrv gnqtdsdggi
       61 dlppvaslpv irllqpmvdg agneefhyae ddwepplvde pqspaaavgk sgttpeaieq
      121 esnlffptle vat
//
LOCUS       XP_077516563             114 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126417 [Amblyomma americanum].
ACCESSION   XP_077516563
VERSION     XP_077516563.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660437.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 20% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..114
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..114
                     /product="uncharacterized protein LOC144126417"
                     /calculated_mol_wt=12256
     CDS             1..114
                     /gene="LOC144126417"
                     /coded_by="XM_077660437.1:138..482"
                     /db_xref="GeneID:144126417"
ORIGIN      
        1 mmmflkdqvd igstfsnshv qdgetaesvl esiysetedg aeppedgafs plvpsrstsp
       61 ephtpngssq astaartlaa prsctgkktk ersrwqddie kvdalitedk dqar
//
LOCUS       XP_077516564             360 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126433 [Amblyomma americanum].
ACCESSION   XP_077516564
VERSION     XP_077516564.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660438.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..360
                     /product="uncharacterized protein LOC144126433"
                     /calculated_mol_wt=38328
     Region          85..212
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     Site            order(89..92,121..123,126,151,190,208)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260008"
     Region          <271..307
                     /region_name="zf-RVT"
                     /note="zinc-binding in reverse transcriptase; pfam13966"
                     /db_xref="CDD:433612"
     CDS             1..360
                     /gene="LOC144126433"
                     /coded_by="XM_077660438.1:1..1083"
                     /db_xref="GeneID:144126433"
ORIGIN      
        1 mlqtegpscl gsaehrtpaw gsciahstgs sappvnqapf pppptrpplp isvdirscsr
       61 rrtpvaalqq ttatllqesl gghlqvytdg svlpatgqaa aacvapalga alscrlrfpa
      121 nstaaelagl hlaadlllll pgqqpavilt dsraalqllr qhqprqhtva nltarlmaiq
      181 dagrpvslqw lpshvgitgn egadqlagaa hsngspvssk vtqldfarpa lrqavralhp
      241 darvasgvrf mrvpdclprr ertlilrlrt gcswtqarlh rhgrapspac sfcgadetlg
      301 hllcacpnle aarrdmtagy rnlrlpsysd qdllhpersq teafrlllef lqstglatrl
//
LOCUS       XP_077516565             333 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126513 [Amblyomma americanum].
ACCESSION   XP_077516565
VERSION     XP_077516565.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660439.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 45% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..333
                     /product="uncharacterized protein LOC144126513"
                     /calculated_mol_wt=36641
     Region          218..312
                     /region_name="RT_RNaseH_2"
                     /note="RNase H-like domain found in reverse transcriptase;
                     pfam17919"
                     /db_xref="CDD:465567"
     CDS             1..333
                     /gene="LOC144126513"
                     /coded_by="XM_077660439.1:40..1041"
                     /db_xref="GeneID:144126513"
ORIGIN      
        1 mascldadsg lpkemlcdap ssagdshgne tadqpleegh kemiekpval feavdgqvli
       61 gpqlwmpaak wiyvmkntsv akcclevarh lwtlseaaer sltgqqcrsi ataarklpat
      121 pekvevmknc layfvdrhhv pelpkdhrva avrkhlrsff teaarqgkrv rgaaaprdtq
      181 tneltlaarc svekfshiae pltletkadv tfkleapqad aftelqrhll sapglgqfde
      241 naktkihafa ssmglgtvhi qktdglekvl kyasrslska eanysttake claiiwamsk
      301 fspylyrrpf nivgdqqmlc wlaslkhpss rla
//
LOCUS       XP_077516566             222 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126529 [Amblyomma americanum].
ACCESSION   XP_077516566
VERSION     XP_077516566.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660440.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 41% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..222
                     /product="uncharacterized protein LOC144126529"
                     /calculated_mol_wt=24125
     CDS             1..222
                     /gene="LOC144126529"
                     /coded_by="XM_077660440.1:100..768"
                     /db_xref="GeneID:144126529"
ORIGIN      
        1 mlrtkytslq cvslhgwccg rctiknfgag ggtlecrpms patelvgava dhmatrlpnp
       61 fdsdgahvge avlslppaal leamvngnet sqddvpaqsp llnslpined elpvqeecln
      121 asrrtvvvsn qsssegippt qtartraggp rvaavertla pevatritai eaeeqrkeel
      181 hqldvqlrrs qlaeqrlkvk mqrkllsldi qirkkqleam qr
//
LOCUS       XP_077516567             578 aa            linear   INV 25-NOV-2025
DEFINITION  proto-oncogene serine/threonine-protein kinase mos [Amblyomma
            americanum].
ACCESSION   XP_077516567
VERSION     XP_077516567.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660441.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..578
                     /product="proto-oncogene serine/threonine-protein kinase
                     mos"
                     /calculated_mol_wt=63625
     Region          329..562
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(332..335,338,340,351,353,375,392..395,438,442..443,
                     445,454..455)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..578
                     /gene="Mos"
                     /coded_by="XM_077660441.1:1..1737"
                     /db_xref="GeneID:144126545"
ORIGIN      
        1 maetyqfter easehlchkd delhtdddda dhlalsdaia ksileseewp syverlrqvg
       61 dddehieell raerpcqqyp vwhahhaile nagttlppge eaaaadlpss slqehtelqt
      121 tlqvsvradd srdanasqlf pdlsrssisd pqqgtcfkpd hstksssrsv flsspftplk
      181 kldiieedvg nreskrpspy nkppqgtpav masrfalgrs ksrksetgsr gttqsknspe
      241 wspdieqlrg sfvssgssge gdlfkssfri sksgssilal erlfleafea ssqaefcmaa
      301 skdqrfalpg tsaqrqagas cttskglplq rlgrgafgrv fgvqikgrlv avkdvrnvda
      361 pseqhasqlv hrhvvrtlcv apleprrflv vmeyggprtl qhlldergpl gtldvcrfgr
      421 qmasalhych rnnvlhldvk psnvlvhngd ckladfgssa irgskpkvcg tvqymapevl
      481 igqppefsad iyslgvvlwq lqtgsrpfdd lhqhavmfqv vrlrarpkfg eddgvpelre
      541 lvkrcwshes derpsvgavl lelsalhket lksravpg
//
LOCUS       XP_077516568             225 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126577 [Amblyomma americanum].
ACCESSION   XP_077516568
VERSION     XP_077516568.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660442.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..225
                     /product="uncharacterized protein LOC144126577"
                     /calculated_mol_wt=25626
     CDS             1..225
                     /gene="LOC144126577"
                     /coded_by="XM_077660442.1:1..678"
                     /db_xref="GeneID:144126577"
ORIGIN      
        1 mryiikeqpe arttymvird iklaegyddi ingikgpsal mnlggpdlvn gfsveymhsv
       61 llgvvrqvte tilstsnsgq hfyvgrpstl edidsrllai kpphcvtrlp rpirelghwk
      121 asewrqwilf yalpclegil hpdywrhlck lsealhillr eeltlseiak tgnllhsffi
      181 qckrlygvas ltfnvhvllh lancarslgs lwahfyfvlk vemdt
//
LOCUS       XP_077516569             558 aa            linear   INV 25-NOV-2025
DEFINITION  tenascin-like [Amblyomma americanum].
ACCESSION   XP_077516569
VERSION     XP_077516569.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660443.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 24% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..558
                     /product="tenascin-like"
                     /calculated_mol_wt=60542
     CDS             1..558
                     /gene="LOC144126609"
                     /coded_by="XM_077660443.1:1..1677"
                     /db_xref="GeneID:144126609"
ORIGIN      
        1 mdimivgesp srslrkwagp kewsgvlsty avtvctsfsa cdaeagdmdc tnyetavmkl
       61 qfdstadiay cvmvtattrc ggdvirsrpa atvvrtpafr ppdvskvkvt avgndsftvs
      121 wqrpegqfdy ywmevtdsna vlgssehrhv gscvsgtllr phqtqvscsq vksctnaslt
      181 iythvngppe qtshgvtveg ifiprkdlhp prnitfawks ssltrvqlnp savawapigt
      241 ysveicsghg tcdaaeklvs caqsetskpw mefesiagte ycvlvsssar crsgvvrspp
      301 asatfrtppf vppdvtnmtl edigvdsftv swerpkghfd ynwvavtdde gssgrsehfh
      361 ldscrngtil hpeqtritcg hleacnnmss nirthsngpp artsagvtlg gifipgaapg
      421 dfradvtats ntsvqivihv sdvksclldd crgycyrlyg gdqhsgitsl avtttdspll
      481 spwmalkvtl invheaqnmw kfkflyfdtp ksfvrsltra arcsvqdhyc pppslfttga
      541 mvqreigcld sctpiets
//
LOCUS       XP_077516570             130 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126625 [Amblyomma americanum].
ACCESSION   XP_077516570
VERSION     XP_077516570.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660444.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 24% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..130
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..130
                     /product="uncharacterized protein LOC144126625"
                     /calculated_mol_wt=14237
     CDS             1..130
                     /gene="LOC144126625"
                     /coded_by="XM_077660444.1:1..393"
                     /db_xref="GeneID:144126625"
ORIGIN      
        1 mlpfielhsf aekyssqlry galgsrgvsd acaaasftal vatgsawyms alfqrrltwa
       61 mpcwmhtfgl qtssclpsvq qetswpafci avlysdisid paglfsvlev flrpnlcrvh
      121 sqkcgdqapy
//
LOCUS       XP_077516571             268 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126641 [Amblyomma americanum].
ACCESSION   XP_077516571
VERSION     XP_077516571.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660445.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 21% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..268
                     /product="uncharacterized protein LOC144126641"
                     /calculated_mol_wt=28980
     CDS             1..268
                     /gene="LOC144126641"
                     /coded_by="XM_077660445.1:1..807"
                     /db_xref="GeneID:144126641"
ORIGIN      
        1 makrdvktpe qkeeriasfs atllrilrlv levrqqqrdl mqrvqrieen mqlrsdrqtd
       61 tgsarpsspl lppstlalpa ytveelesae aaiedesvaa gsesswsawg gkglkevaan
      121 vmtaimgvav qrlysrhghk eknwfishkl ckvatdaice ktgvdvaeaq gfiikwlpgs
      181 vdrsggrkrr faeaflpdta aslatavnpc asapstvsha apstvshaap ppkippptwp
      241 pppripphaw pslppkvppr aphslarv
//
LOCUS       XP_077516572             298 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126657 [Amblyomma americanum].
ACCESSION   XP_077516572
VERSION     XP_077516572.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660446.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..298
                     /product="uncharacterized protein LOC144126657"
                     /calculated_mol_wt=33900
     Region          3..56
                     /region_name="PHD_SF"
                     /note="PHD finger superfamily; cd15489"
                     /db_xref="CDD:276966"
     Site            order(3,17..20,24,51)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:276966"
     CDS             1..298
                     /gene="LOC144126657"
                     /coded_by="XM_077660446.1:312..1208"
                     /db_xref="GeneID:144126657"
ORIGIN      
        1 mlrccacrqp vddscqslcc seceftyhvg scsgvseetf iskrakawka wrcetckgsr
       61 kaedtlhgqq sdrelitrlf smeskidsll amssklesle ssvsllsdkf defqtrllaq
      121 ekstkdvtkr verlekaewa ceiaqlnkdv dslewrsrrl nieihglpet enedllkkvn
      181 eigtklelde vcssditalh rlpakpgktr gvivrfakqe vrdawlakrq alrddntgky
      241 mcenmtrftr tlltvakewa kesgyafvwh vngkvlvrkq agaravvird kddlanlr
//
LOCUS       XP_077516573             207 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126673 [Amblyomma americanum].
ACCESSION   XP_077516573
VERSION     XP_077516573.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660447.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..207
                     /product="uncharacterized protein LOC144126673"
                     /calculated_mol_wt=23680
     CDS             1..207
                     /gene="LOC144126673"
                     /coded_by="XM_077660447.1:1..624"
                     /db_xref="GeneID:144126673"
ORIGIN      
        1 mssklesles svsllsdkfd efqtrllaqe kstkdvtkrv erhekaewac eiaqlnkdvd
       61 slewrsrrln ieihglpete nedllkkvne igtkleldev cssditalhr lpakpgktrg
      121 vivrfakqev rdawlakrqa lrddntgkym cenmtrytrt lltvakewak esgyafvwhv
      181 ngkvlvrkqa garavvirdk ddlanlr
//
LOCUS       XP_077516574             294 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126689 [Amblyomma americanum].
ACCESSION   XP_077516574
VERSION     XP_077516574.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660448.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..294
                     /product="uncharacterized protein LOC144126689"
                     /calculated_mol_wt=31718
     Region          <201..>235
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     CDS             1..294
                     /gene="LOC144126689"
                     /coded_by="XM_077660448.1:1..885"
                     /db_xref="GeneID:144126689"
ORIGIN      
        1 mssggaataa lagrgnritd sddtdyqlil pqlptgsivl ntvflhgdvr arpyrvedfr
       61 dalvptgllp dvvalgayqv nhvwavtlng akatrrllaf kelkvknrrc viidpqdhqv
      121 rlslhwllhg vadedvrsal vafgivmdvs rerwlvqgms dkgsmtrtav lklkmgmtve
      181 dlpheirvgg elalvvapgr pmqclrchgt ghvrreckvp rcsncrrfgh edsqcvrtya
      241 samgpgesdg aehlmdatea edaakgtgep lptavaeqaa lagepqdtsa erps
//
LOCUS       XP_077516575             328 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126705, partial [Amblyomma
            americanum].
ACCESSION   XP_077516575
VERSION     XP_077516575.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660449.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: incomplete on the carboxy end.
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..>328
                     /product="uncharacterized protein LOC144126705"
     CDS             1..328
                     /gene="LOC144126705"
                     /coded_by="XM_077660449.1:331..>1314"
                     /db_xref="GeneID:144126705"
ORIGIN      
        1 mthrenvpsp ahghnrrsrq rgrtmavsap atpvalrplh qscvrlrkqe ekgwgtglhd
       61 difkmpygrh taaraltrhl kkmheteslc fdtedniafk sscsstiwrq wkftngcswd
      121 rrvrrnliqg rkrqtlaala sctfflpggp nrscekalfa qvallsapvd sspksfkymv
      181 etrqpttegs gspyrrnslt pqkqglfltq ednlldglar sqvemaasva vpqnsfandd
      241 edvgtqdaaa eagrrgalrn layvfypvlv vfwllrwlcs qvlvvfafvl rgsssgvere
      301 shltaadgte vmaaasgepd ayllirqk
//
LOCUS       XP_077516576             207 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126736 [Amblyomma americanum].
ACCESSION   XP_077516576
VERSION     XP_077516576.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660450.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..207
                     /product="uncharacterized protein LOC144126736"
                     /calculated_mol_wt=23684
     CDS             1..207
                     /gene="LOC144126736"
                     /coded_by="XM_077660450.1:1..624"
                     /db_xref="GeneID:144126736"
ORIGIN      
        1 mssklesles svsllsdkfd efqtrllaqe rstkdvtkrv erlekaewac eiaqlnkdvd
       61 slewrsrrln ieihglpete nedllkkvne igtkleldev cssditalhr lpakpgktrg
      121 vivrfakqev rdawlakrqa lrddntgkym cenmtrytrt lltvakewak esgyafvwhv
      181 ngkvlvrkqa garavvirdk ddlanlr
//
LOCUS       XP_077516577             293 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126752 [Amblyomma americanum].
ACCESSION   XP_077516577
VERSION     XP_077516577.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660451.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..293
                     /product="uncharacterized protein LOC144126752"
                     /calculated_mol_wt=33302
     Region          3..56
                     /region_name="PHD"
                     /note="PHD zinc finger; smart00249"
                     /db_xref="CDD:214584"
     Site            order(3,17..20,24,51)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:276966"
     CDS             1..293
                     /gene="LOC144126752"
                     /coded_by="XM_077660451.1:257..1138"
                     /db_xref="GeneID:144126752"
ORIGIN      
        1 mlrccacrqp vddscqslcc seceftyhvg scsgvseetf iskrakawka wrcetckgsr
       61 kaedtlhgqq sdrelitrlf smeskidsll amssklesle ssvsllsdkf defqtrllaq
      121 ekstkdvtkr verlekaewa ceiaqlnkdv dslewrsrrl nieihglpet enedllkkvn
      181 eigtklelde vcssditalh rlpakpgktr gvivrfakqe vrdawlakrq alrddntgky
      241 mcenmtrytr tlltvakewg kesgyafvwh vngkvlvpvh eqlssairmt spi
//
LOCUS       XP_077516578             228 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126769 [Amblyomma americanum].
ACCESSION   XP_077516578
VERSION     XP_077516578.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660452.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..228
                     /product="uncharacterized protein LOC144126769"
                     /calculated_mol_wt=24231
     CDS             1..228
                     /gene="LOC144126769"
                     /coded_by="XM_077660452.1:206..892"
                     /db_xref="GeneID:144126769"
ORIGIN      
        1 mqrvqrieen mqlrsdrqtd tgsarpsspl lppstlalpa ytveelesae aaiedesvaa
       61 gsesswsawg gkglkevaan vmtaimgvav qrlysrhghk eknwfishkl ckvatdaice
      121 ktgvdvakaq gfiikwlpgs vdrsggrkrr faeaflpdta aslatavnpc asapstvsha
      181 apstvshaap ppkippptwp pppripphaw pslppkvppr aphslarv
//
LOCUS       XP_077516580             142 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126849 [Amblyomma americanum].
ACCESSION   XP_077516580
VERSION     XP_077516580.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660454.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..142
                     /product="uncharacterized protein LOC144126849"
                     /calculated_mol_wt=16149
     CDS             1..142
                     /gene="LOC144126849"
                     /coded_by="XM_077660454.1:210..638"
                     /db_xref="GeneID:144126849"
ORIGIN      
        1 mqrfsfetgr klkktffkks lniffwkkgf llsptlhpql nlhckaisee ktalcasala
       61 nflrdsnkke vlyyyvqmel eesrgnrgak eaaflpllaa lfkddvgqlf rvfeegtsit
      121 erlhelpctp tvvahgklly ts
//
LOCUS       XP_077516581             142 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144126927 [Amblyomma americanum].
ACCESSION   XP_077516581
VERSION     XP_077516581.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660455.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..142
                     /product="uncharacterized protein LOC144126927"
                     /calculated_mol_wt=16331
     CDS             1..142
                     /gene="LOC144126927"
                     /coded_by="XM_077660455.1:85..513"
                     /db_xref="GeneID:144126927"
ORIGIN      
        1 mhrfesdlls tltftmvgtd dvfkklgpwh ypvmafcflr gfpaayhama ptfiapqlkh
       61 wcakpaqltn wstemwlekg vpweerngia qpsrcemyaf eealdgtprf lnesrlecss
      121 weydlgdytd tltntspter lh
//
LOCUS       XP_077516583             206 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077516583
VERSION     XP_077516583.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660457.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 14% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..206
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=23058
     Region          <1..162
                     /region_name="Piwi-like"
                     /note="PIWI domain. Domain found in proteins involved in
                     RNA silencing. RNA silencing refers to a group of related
                     gene-silencing mechanisms mediated by short RNA molecules,
                     including siRNAs, miRNAs, and heterochromatin-related
                     guide RNAs. The central component...; cl00628"
                     /db_xref="CDD:412485"
     CDS             1..206
                     /gene="LOC144127022"
                     /coded_by="XM_077660457.1:1..621"
                     /db_xref="GeneID:144127022"
ORIGIN      
        1 mikelilafh ratrhkperi ifyrdgvsai rlackelspn etyepaitfi vvqkrhhtrf
       61 mpandrdgvg kcrnvppgtt vdsvvthpld fdfflcshfg iqgtsrpahy yvvwddsmfs
      121 addlqklsfy lchtysrcar svsipapvyy ahlaafrakh hiaskletsg avsqsseggg
      181 dtvlttaqyv eavkvlqelq asmyfv
//
LOCUS       XP_077516584             260 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077516584
VERSION     XP_077516584.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660458.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 32% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..260
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=28970
     Region          <77..216
                     /region_name="Piwi-like"
                     /note="PIWI domain. Domain found in proteins involved in
                     RNA silencing. RNA silencing refers to a group of related
                     gene-silencing mechanisms mediated by short RNA molecules,
                     including siRNAs, miRNAs, and heterochromatin-related
                     guide RNAs. The central component...; cl00628"
                     /db_xref="CDD:412485"
     CDS             1..260
                     /gene="LOC144127038"
                     /coded_by="XM_077660458.1:1..783"
                     /db_xref="GeneID:144127038"
ORIGIN      
        1 mlpclhllcq scsegagakr nhcpfhkepf qeedvvwsaf trdsifgrkv rcwnaehgcd
       61 aevvesamle hfanacqfhv vsairlacke lspnetyepa itfivvqkrh htrfmptndr
      121 dgvgkcrnvp pgttvdsvvt hpldfdfflc shfgiqgtsr pahyyvvwdd smfsaddlqk
      181 lsfylchtys rcarsvsipa pvyyahlaaf rakhhiaskl etsgavsqss egggdtvltt
      241 aqyveavkvl qelqasmyfv
//
LOCUS       XP_077516585             183 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-4-like [Amblyomma americanum].
ACCESSION   XP_077516585
VERSION     XP_077516585.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660459.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="protein argonaute-4-like"
                     /calculated_mol_wt=20211
     Region          <4..139
                     /region_name="Piwi-like"
                     /note="PIWI domain. Domain found in proteins involved in
                     RNA silencing. RNA silencing refers to a group of related
                     gene-silencing mechanisms mediated by short RNA molecules,
                     including siRNAs, miRNAs, and heterochromatin-related
                     guide RNAs. The central component...; cl00628"
                     /db_xref="CDD:412485"
     CDS             1..183
                     /gene="LOC144127054"
                     /coded_by="XM_077660459.1:1..552"
                     /db_xref="GeneID:144127054"
ORIGIN      
        1 mkgvsairla ckelspnety epaitfivvq krhhtrfmpa ndrdgvgkcr nvppgttvds
       61 vvthpldfdf flcshfgiqg tsrpahyyvv wddsmfsadd lqklsfylch tysrcarsvs
      121 ipapvyyahl aafrakhhia skletsgavs qssegsgdtv lttaqyveav kvlqelqasm
      181 yfv
//
LOCUS       XP_077516586             969 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-4-like [Amblyomma americanum].
ACCESSION   XP_077516586
VERSION     XP_077516586.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660460.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 27% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..969
                     /product="protein argonaute-4-like"
                     /calculated_mol_wt=108041
     Region          <21..51
                     /region_name="YjgF_YER057c_UK114_family"
                     /note="YjgF, YER057c, and UK114 belong to a large family
                     of proteins present in bacteria, archaea, and eukaryotes
                     with no definitive function. The conserved domain is
                     similar in structure to chorismate mutase but there is no
                     sequence similarity and no...; cl10015"
                     /db_xref="CDD:447879"
     Region          391..>969
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     CDS             1..969
                     /gene="LOC144127070"
                     /coded_by="XM_077660460.1:1..2910"
                     /db_xref="GeneID:144127070"
ORIGIN      
        1 maptslqtvf gfdrgfdwrp tcfvnpfppt rvcivcglvp ssaamlpclh llcqsysega
       61 gakrnhcpfq kepfqeedvv wsaftrdsil grkvrcwnae hgcdtevvas amlehfanac
      121 qfhvswrhsv cqpcydgaaa krshcpldke pfqeedmawt tisrgnvlgr kscsegagak
      181 rnhcpfhkep fqeedvvwsa ftrdsilgrk vrcwnaehgc daevvasaml ehfanacqfh
      241 vvrcpecses vrhrdiaehl apvpsasggn qatppapspa gpswvsfaqa lqqgaaaspq
      301 pravsggptt aadpsahpta qpsasrpsyg ataaagsakv asssprpasa aaavsgtqpa
      361 aaalppspps tppvavtqqt paagdqatsg ddvrikeler tlpshfprrp ahgqlgrtiq
      421 lianhfsiei ptgsvyhydv dissetaket kvpeqkkyrc lstkinrivi ellvkkyrqd
      481 lancipafdg rknlytrrql nfrertftvd leedqrsqkf iikiqyaatv nldalhgvfq
      541 krvqtvpqev lqaidivlrh spsinlapvg rsffrppgpn ehndlgggre vwfgyytsvr
      601 paqwkpmlnv dmsattfyes lplvdfmcrf lsdsrrvltp adfrslrdnq yvrlnrelkg
      661 lrvkvthlpy prkykvvkit repakeiyfe segsqisvad yfqsryrrls ypnfpcvqsg
      721 spthpvyipl evcelaegqh crkkldesqt aemikrtakp pakrfqkirq svrdmvsssd
      781 kylrefgvki nteptqvvgr vldppslvfe nntlckprdg twdlrgqrfy kamsmtkwiv
      841 lnvsrfahkd slenfikmli rigqelgmri aqplavitfd tnrqpmrtvl teqrkqytql
      901 emvvavitka tnyaeikqva etelslrtqc ildtnvvqkc naaliqnlcq kinakmggin
      961 nsllmqekp
//
LOCUS       XP_077516587             652 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-4-like [Amblyomma americanum].
ACCESSION   XP_077516587
VERSION     XP_077516587.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660461.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 30% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..652
                     /product="protein argonaute-4-like"
                     /calculated_mol_wt=72800
     Region          219..>651
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     CDS             1..652
                     /gene="LOC144127086"
                     /coded_by="XM_077660461.1:1..1959"
                     /db_xref="GeneID:144127086"
ORIGIN      
        1 mawttisrgk vlgrkvrrwn sehgcdaecv asamlehfan tyqyhamvsp cscsegagak
       61 rnhcpfhkep fqeedvvwsa ftrdsilgrk vrcwnaehgc daevvasaml ehfanacqfh
      121 vgaaaspqqr avsggpttaa dpsahptaqp sasrpsygat adagsakvas ssprpasaaa
      181 avsgtqpaaa alppsppstp pvavtqqtpa agdqatsgdd vrikelertl pshfprrpah
      241 gqlgrtiqll anhfsieipt gsvyhydvdi ssetaketkv peqkkyrcls tkinriviel
      301 llkkyrqdla ncipafdgrk nlytrrqlnf rertftvdle edqrsqkfii kiqyaatvnl
      361 dalhgvfqkr vqtvpqevlq tidivlrhsp sinlapvgrs ffrppgpneh ndlgggrevw
      421 fgyytsvrpa qwkpmlnvdm sattfyeslp lvdfmcrfls dsrrvltpad frslrdnqyv
      481 rlnrelkglr vkvthlpypr kykvvkitre pakeiyfese gsqisvadyl qsryrrlsyp
      541 nfpcvqsgsp thpvyiplev celaegqhcr kkldesqtae mikrtakppa krfqkirqsv
      601 rdmvsssdky lrefgvkint eptqvvgrvl dppslvfenn tmckprdstw dl
//
LOCUS       XP_077516588             183 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-4-like [Amblyomma americanum].
ACCESSION   XP_077516588
VERSION     XP_077516588.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660462.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="protein argonaute-4-like"
                     /calculated_mol_wt=20211
     Region          <4..139
                     /region_name="Piwi-like"
                     /note="PIWI domain. Domain found in proteins involved in
                     RNA silencing. RNA silencing refers to a group of related
                     gene-silencing mechanisms mediated by short RNA molecules,
                     including siRNAs, miRNAs, and heterochromatin-related
                     guide RNAs. The central component...; cl00628"
                     /db_xref="CDD:412485"
     CDS             1..183
                     /gene="LOC144127102"
                     /coded_by="XM_077660462.1:1..552"
                     /db_xref="GeneID:144127102"
ORIGIN      
        1 mkgvsairla ckelspnety epaitfivvq krhhtrfmpa ndrdgvgkcr nvppgttvds
       61 vvthpldfdf flcshfgiqg tswpahyyvv wddsmfsadd lqklsfylch tysrcarsvs
      121 ipapvyyahl aafrakhhia skletsgavs qssegggdtv lttaqyveav kvlqelqasm
      181 yfv
//
LOCUS       XP_077516589             311 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127118 [Amblyomma americanum].
ACCESSION   XP_077516589
VERSION     XP_077516589.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660463.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..311
                     /product="uncharacterized protein LOC144127118"
                     /calculated_mol_wt=32521
     Region          225..267
                     /region_name="MSL2_CXC"
                     /note="DNA-binding cysteine-rich domain of male-specific
                     lethal 2 and related proteins; cl25608"
                     /db_xref="CDD:452415"
     CDS             1..311
                     /gene="LOC144127118"
                     /coded_by="XM_077660463.1:1..936"
                     /db_xref="GeneID:144127118"
ORIGIN      
        1 msvgsrlckm cvgqtmrlns pcaecnssta gftqnlqlri vlqcykkicr ylsapsfqkk
       61 wgslnggmdi tfrdivvkgs rladdyrhae ptstpstssa vpflaasssf ssslasagva
      121 assttvppqa arvvnrtsav kaaavtksvn gdrtrrvall ngdvmalahd hayaapykli
      181 kvppvlelss kgsdsseysg ttstaalpta avlhypqppv sasgrrgclc gaasinpgnl
      241 tclgqrcpcy vagsscakcr ckgcrnpidk itqiwgealq vgahesaksg adgkppkgrt
      301 hvvhilhldh k
//
LOCUS       XP_077516590             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127134 [Amblyomma americanum].
ACCESSION   XP_077516590
VERSION     XP_077516590.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660464.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 33% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144127134"
                     /calculated_mol_wt=12817
     CDS             1..127
                     /gene="LOC144127134"
                     /coded_by="XM_077660464.1:56..439"
                     /db_xref="GeneID:144127134"
ORIGIN      
        1 mgvrlanpfd sdrhqadpgg esqptpsvaa llsrtqdgsi pgasgdaedp lwswhyeegs
       61 pqqdeqmeee pvamaaslts attatatpsv lpaastaraa evlsppppss sslasveels
      121 slsdggt
//
LOCUS       XP_077516591             296 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127165 [Amblyomma americanum].
ACCESSION   XP_077516591
VERSION     XP_077516591.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660465.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 43% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..296
                     /product="uncharacterized protein LOC144127165"
                     /calculated_mol_wt=30969
     Region          210..252
                     /region_name="MSL2_CXC"
                     /note="DNA-binding cysteine-rich domain of male-specific
                     lethal 2 and related proteins; cl25608"
                     /db_xref="CDD:452415"
     CDS             1..296
                     /gene="LOC144127165"
                     /coded_by="XM_077660465.1:1..891"
                     /db_xref="GeneID:144127165"
ORIGIN      
        1 mrlnspcaec nsstagftqn lqlrivlqcy kkicrylsap sfqkkwgsln ggmditfrdi
       61 vvegsrladd yrhaeptstp stssavpfla asssfsssla sagvaasstt vppqaarvvn
      121 rtsavkaaav tksvngdrtr rvallngdvm alahdhayaa pyklikvppv lelssngsds
      181 seysgttsta alptaavlhy pqppvsasgr rgcrcgaasi npgnltclgq rcpcyvagss
      241 cakcrckgcr npidkitqiw gealqvgahe saksgadgkp pkgrthvvhi lhldhk
//
LOCUS       XP_077516592             243 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127181 [Amblyomma americanum].
ACCESSION   XP_077516592
VERSION     XP_077516592.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660466.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 44% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..243
                     /product="uncharacterized protein LOC144127181"
                     /calculated_mol_wt=26185
     Region          8..85
                     /region_name="GT1"
                     /note="myb-like, SANT family; cl23759"
                     /db_xref="CDD:474047"
     CDS             1..243
                     /gene="LOC144127181"
                     /coded_by="XM_077660466.1:1..732"
                     /db_xref="GeneID:144127181"
ORIGIN      
        1 mektekgkin csrveralll dlvdkhkavl enkrtdavsv arnrkqweli etqfnsshnl
       61 sprtwlqlkk cwenwknkwr kakaddnrei fktgggsaap sqltedlqrv gsvashmgvr
      121 lanpfdsdrh qadpggesqp tpsvaallsr tqdgsipgas gdaedplwsw hyeegspqqd
      181 eqmeeepvam aasltsatta tatpsvlpaa staraaevls ppppssssla sveelsslsd
      241 ggt
//
LOCUS       XP_077516593             142 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127212 [Amblyomma americanum].
ACCESSION   XP_077516593
VERSION     XP_077516593.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660467.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..142
                     /product="uncharacterized protein LOC144127212"
                     /calculated_mol_wt=16119
     Region          <1..115
                     /region_name="COG3335"
                     /note="Transposase [Mobilome: prophages, transposons]"
                     /db_xref="CDD:442564"
     CDS             1..142
                     /gene="LOC144127212"
                     /coded_by="XM_077660467.1:1..429"
                     /db_xref="GeneID:144127212"
ORIGIN      
        1 mdgnyfekwf tdkllpnipa nsivvmdnap yhsvalekap tkttckadiq lwltkkgvpr
       61 sqdmvgaell elsqkvntps vvyridtlaa thghkvlrlp pyhcefnpie lvwsqsrdts
      121 qvatnvslwq kwksscqnhw rl
//
LOCUS       XP_077516595             183 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127293 [Amblyomma americanum].
ACCESSION   XP_077516595
VERSION     XP_077516595.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660469.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="uncharacterized protein LOC144127293"
                     /calculated_mol_wt=20853
     CDS             1..183
                     /gene="LOC144127293"
                     /coded_by="XM_077660469.1:1..552"
                     /db_xref="GeneID:144127293"
ORIGIN      
        1 mryiikeqpe arttymviry mklaegyddi ingfkgpsal mnlggkdlvn gfsveymhsv
       61 llgvvrqvte tilstsnsgq hfyvgrpstl edidsrllai khphcvtrlp rpirerghwk
      121 asewrqwilf nalpclegil hpdywrhlck lsealhillr eeltlseiak agnllqvsfv
      181 nia
//
LOCUS       XP_077516596             190 aa            linear   INV 25-NOV-2025
DEFINITION  mitotic spindle and nuclear protein [Amblyomma americanum].
ACCESSION   XP_077516596
VERSION     XP_077516596.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660470.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..190
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..190
                     /product="mitotic spindle and nuclear protein"
                     /calculated_mol_wt=21045
     Region          <30..>144
                     /region_name="PTZ00449"
                     /note="104 kDa microneme/rhoptry antigen; Provisional"
                     /db_xref="CDD:185628"
     CDS             1..190
                     /gene="Mink"
                     /coded_by="XM_077660470.1:452..1024"
                     /db_xref="GeneID:144127309"
ORIGIN      
        1 mwprvaalkt rissqrkris prnaghhmds lpegrkpdke apqgnhtpgt trtrrpskvk
       61 kppnfarlhe lefkkmksit dmpmrrklpe stktmparks algsarvtas pgneasgtrp
      121 psggtisekp vpmnmepsge ppkkatplra dvadhmckrr eqrrnelstv radgrsdvvn
      181 akrgkfphlk
//
LOCUS       XP_077516597             278 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127356 [Amblyomma americanum].
ACCESSION   XP_077516597
VERSION     XP_077516597.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660471.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..278
                     /product="uncharacterized protein LOC144127356"
                     /calculated_mol_wt=31443
     CDS             1..278
                     /gene="LOC144127356"
                     /coded_by="XM_077660471.1:1..837"
                     /db_xref="GeneID:144127356"
ORIGIN      
        1 mlaeersavn ilrrnqgivi lpadkgnatv mlnrpdyekk mrdllqdrst yvalkkdpts
       61 klerelqkll vdvfhfvpph hksvyfrllc qngsapalyd lpkihkegvp mgrivdftrf
      121 llyellgylh rvfaplvgkg stfvrnsyef iekvcdisvd dddlmvsfdv kslftsvpvd
      181 lavdvcgaal hsdkklgert pfealdqlrl lkfclentyf vfrgtfykev hgtamgaais
      241 vtaanltlea iedqalasfq prpkvflryi ddcfciik
//
LOCUS       XP_077516598             300 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127372 [Amblyomma americanum].
ACCESSION   XP_077516598
VERSION     XP_077516598.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660472.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 47% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..300
                     /product="uncharacterized protein LOC144127372"
                     /calculated_mol_wt=32873
     Region          <186..247
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..300
                     /gene="LOC144127372"
                     /coded_by="XM_077660472.1:1..903"
                     /db_xref="GeneID:144127372"
ORIGIN      
        1 manasaipgd gpssavsals lrlplfspad pqlwlaqvns qftigcntth ekkfhhvaas
       61 lppeisaevr dllinsyata pfdtlaaeli krtavsehrr lqqlisseel vdrkptqslr
      121 rlqqllgdka ttfdqaflle vflqrlpssv rlvlvpaqgl sleklaqlad svmhvscyti
      181 sldavsaads rystfgrell amyisvrqfk hllegrqfyi vtdhkrlifv iksgrgldns
      241 padalsrvta ltsaapprnl dfaalekaqq haskvmdlcq nprslvlrei llpscpvpii
//
LOCUS       XP_077516599             134 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127434 [Amblyomma americanum].
ACCESSION   XP_077516599
VERSION     XP_077516599.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660473.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..134
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..134
                     /product="uncharacterized protein LOC144127434"
                     /calculated_mol_wt=15010
     CDS             1..134
                     /gene="LOC144127434"
                     /coded_by="XM_077660473.1:1..405"
                     /db_xref="GeneID:144127434"
ORIGIN      
        1 mnlrpcvlll avavllyala vksseamhhk khkkimkvll ahalfkkrvi pipipfpqes
       61 hhtnivpvpn pipfkvpiht ihkhhhhtkh vhhhkhtkgr ntllildrqh eggnhgggyh
      121 ggggyhggyh gsyw
//
LOCUS       XP_077516600             108 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127450 [Amblyomma americanum].
ACCESSION   XP_077516600
VERSION     XP_077516600.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660474.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..108
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..108
                     /product="uncharacterized protein LOC144127450"
                     /calculated_mol_wt=11329
     CDS             1..108
                     /gene="LOC144127450"
                     /coded_by="XM_077660474.1:110..436"
                     /db_xref="GeneID:144127450"
ORIGIN      
        1 mptlhsgkhv dyhhlnsacn sdvestpqdt vshnaaldca gssswnldsa phhhadehap
       61 dgipiirtrt pmgasgflss qhalgvggag qegispeqqr fnhhivks
//
LOCUS       XP_077516601             246 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127466 [Amblyomma americanum].
ACCESSION   XP_077516601
VERSION     XP_077516601.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660475.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..246
                     /product="uncharacterized protein LOC144127466"
                     /calculated_mol_wt=28173
     Region          <73..124
                     /region_name="HTH_Tnp_Tc3_2"
                     /note="Transposase; pfam01498"
                     /db_xref="CDD:366677"
     CDS             1..246
                     /gene="LOC144127466"
                     /coded_by="XM_077660475.1:1..741"
                     /db_xref="GeneID:144127466"
ORIGIN      
        1 mtqksvfias qetnyeakhg cepfygrish lqgirgdegr irdapregrp pattpdedla
       61 ivaavscelt lslaevrnel gprvslstnr rrlneaglrs rvacqrpwls qrhkqqrlef
      121 trehiswgpe ewrlvifsde stfvsrwdqq qrvwrlidal qelgvqlvsw ppkgadfnli
      181 eqiwgmmkrn lamrtfqhqs kaelwaamse eweklsglad lvprlyasil kriadavhan
      241 gaftrn
//
LOCUS       XP_077516605             344 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase Siah1-like [Amblyomma americanum].
ACCESSION   XP_077516605
VERSION     XP_077516605.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660479.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 43% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..344
                     /product="E3 ubiquitin-protein ligase Siah1-like"
                     /calculated_mol_wt=38105
     Region          7..191
                     /region_name="Sina"
                     /note="Seven in absentia protein family; pfam03145"
                     /db_xref="CDD:460824"
     Region          <268..>319
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            269
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238827"
     CDS             1..344
                     /gene="LOC144127482"
                     /coded_by="XM_077660479.1:78..1112"
                     /db_xref="GeneID:144127482"
ORIGIN      
        1 mngcegtvff pcqffmrgcd allchtneae heqacefrpy vcpfpgvtcq wrgsvrqmms
       61 hfvhwhgcti cegehlaflm esinipcsas wivvqscfgh hfilvlekke rydghqqffa
      121 dvqligsrrq aenftyrlel ighkrrlawe astrsthegv qatsmrsdcl vfdtsmaqrf
      181 aesgnlviev tiflkspard amtrrhgppf iprtkdkpps kpggpstrkp elpltvpppp
      241 radvekrtga pppgssrtwt qamiqgsqga vlsallftla mkkhptllge vpgvqhalya
      301 dditfwgthg sdweaallgc stvldqralv searaaaeat vvpd
//
LOCUS       XP_077516614             673 aa            linear   INV 25-NOV-2025
DEFINITION  lysosomal alpha-mannosidase-like [Amblyomma americanum].
ACCESSION   XP_077516614
VERSION     XP_077516614.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660488.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..673
                     /product="lysosomal alpha-mannosidase-like"
                     /calculated_mol_wt=75980
     Region          <2..>79
                     /region_name="GH38-57_N_LamB_YdjC_SF"
                     /note="Catalytic domain of glycoside hydrolase (GH)
                     families 38 and 57, lactam utilization protein LamB/YcsF
                     family proteins, YdjC-family proteins, and similar
                     proteins; cl49606"
                     /db_xref="CDD:483946"
     Region          <108..>171
                     /region_name="PLN02701"
                     /note="alpha-mannosidase"
                     /db_xref="CDD:178304"
     Region          363..526
                     /region_name="Glyco_hydro_38C"
                     /note="Glycosyl hydrolases family 38 C-terminal domain;
                     cl20343"
                     /db_xref="CDD:418856"
     CDS             1..673
                     /gene="LOC144127494"
                     /coded_by="XM_077660488.1:1..2022"
                     /db_xref="GeneID:144127494"
ORIGIN      
        1 mtthytatvd emslglrlas asgrarvawq idplgharqe aalfaqmgfd glflgrihld
       61 dkdwrqhnkq meflwkadky iasereprar lllacylkal hdsgvwrpef sddffpyadq
      121 ehaywtgyyt srpnffkcfa rrangilqty srapsrfcaa ssllqcashv lspslpekac
      181 kqlkvigqld dsadvdtlgr vnkayrrlll sssggtafqq dicltfcpll nissceitet
      241 adeffvfvyn plstprpagk faltlcrkmt hilvkwkden kwdvypvkmi gdapvgirll
      301 tqenaiselr gqevrvrwee gqdaapaell afdaykmvkr lkgmmakfek spgpctpadk
      361 kcrlfvdpvt gllsrvmlln tglevpfrqs lfayianegv vekssgaysf spaddqpvdl
      421 grrgpllqei hqtfndwash virlykdedl iefdwvigpi pfrrnaqrpw tktiveptay
      481 nyypvfswiy lsneshnlql tvfpdrpqcg sglregelel myddsfgaee plneigvdkr
      541 glvargthrv flgsvedser ilrtmanrmv fgpqfafytr drqlrdipvp rasglqyplp
      601 anlhlptleh ignsraiarl ehlypgvkdn rlnrpaslsl kadpddpsss hspprpsmhs
      661 essastpqpe sps
//
LOCUS       XP_077516625             475 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127504 [Amblyomma americanum].
ACCESSION   XP_077516625
VERSION     XP_077516625.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660499.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 30% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..475
                     /product="uncharacterized protein LOC144127504"
                     /calculated_mol_wt=52543
     CDS             1..475
                     /gene="LOC144127504"
                     /coded_by="XM_077660499.1:1..1428"
                     /db_xref="GeneID:144127504"
ORIGIN      
        1 mgevqthtas taaaataseq evlheedviq tslqpsnepv qylcdassaa eiagpsqsdp
       61 egtttaickd pplaeqhsvs peemaaeqpk eqevlmewei gssgtisaee eipiavsppm
      121 qkkpchrqlp fdrnqsdhea atcgykydyk sdrdydylyy ykydnkhdyd ykydnkhdyh
      181 ykegkieees smpaftagld alwaqsishf gflnaylqya svthvtklln vmktvdrnfw
      241 skqtstrsty malgvapvnp mefsaladll qqvflpqifv aighisfsdd lrpdcvifpp
      301 nvfafpqgft ptyghtmlal glilhgtasg iepteiawvt spvngtrfya cdsltsaefl
      361 rphypgaatc ehlsthdllg apphewatyt lprncigsgl ggttsmrcan pfvfivqgaa
      421 iellqhierv prhpalvlsl tllareyvar rpdrqktpta saplhsstrv tdhaf
//
LOCUS       XP_077516661             396 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127540 [Amblyomma americanum].
ACCESSION   XP_077516661
VERSION     XP_077516661.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660535.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 45% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..396
                     /product="uncharacterized protein LOC144127540"
                     /calculated_mol_wt=43884
     CDS             1..396
                     /gene="LOC144127540"
                     /coded_by="XM_077660535.1:1..1191"
                     /db_xref="GeneID:144127540"
ORIGIN      
        1 masqlttict vqnpcaafgd dirnmlsfdp gaaivedsks kkerllsdva laamsrslis
       61 pvtgwqhdlt aipplsyqsi refcesksct qrtynrsysf hvesyanvaa lntnvskitn
      121 pgskpgtrst sssvnvqves tddacstsnq painemlael lagqrkmaed iadikrfqqr
      181 vnsrfeales rvsalesssp tsngdasdga vqrqlnslse vvsklasrnd elenrsrrnn
      241 lifhglveed eetnkalfia vtkvfidnlq ldcpkieryh rlgrkqearp rpvivklldf
      301 rektqalkna hrlknsgvsl sedfsahvrl irnkiwdatq syrdsgskvh lrydhvfids
      361 vrynwddvek slvkdskstp pdtaaataak ppadsd
//
LOCUS       XP_077516678             309 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127560 [Amblyomma americanum].
ACCESSION   XP_077516678
VERSION     XP_077516678.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660552.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 20% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..309
                     /product="uncharacterized protein LOC144127560"
                     /calculated_mol_wt=34140
     Region          70..152
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     CDS             1..309
                     /gene="LOC144127560"
                     /coded_by="XM_077660552.1:49..978"
                     /db_xref="GeneID:144127560"
ORIGIN      
        1 mqqtghedgm qpeltmastg gklpefdpds gnldvfierf elhitandvs dakklhlfls
       61 aigekgyvtl rslllpgtps tstyknvvte lqkhytptrs vvseryhfnq kkqgpqesvt
      121 dfvvrlkksa ascdfssfle qalrdrliag lhseaircrl lamsdteltw drassiatav
      181 emaakdtlam vsettcqhss sgsdihwqsk ssaspkgsev rvcschapdk pkpktstqrt
      241 ksdchrcvgr dasiicdvpp ltmkhkligk cayftpptlp prrclplsrr leldrafqtt
      301 shlitnqfc
//
LOCUS       XP_077516680             203 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127570 [Amblyomma americanum].
ACCESSION   XP_077516680
VERSION     XP_077516680.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660554.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 18% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..203
                     /product="uncharacterized protein LOC144127570"
                     /calculated_mol_wt=23080
     Region          61..152
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     CDS             1..203
                     /gene="LOC144127570"
                     /coded_by="XM_077660554.1:1..612"
                     /db_xref="GeneID:144127570"
ORIGIN      
        1 mpndqavqvs paavicagal rqrdpaifsg tddhdvedwl asyervsayn kwyaeikldn
       61 vifcltgvan lwyrnheadm tswsvfksnf sevfgrpsvr klraeqrlra rsqqvgenft
      121 syiedvvdlc krvnaqmpea dkirhilkgi dddafqmlla rdvrtvaevv plcqsydelr
      181 kqraltreht tnvdslagln dvw
//
LOCUS       XP_077516725             146 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127639 [Amblyomma americanum].
ACCESSION   XP_077516725
VERSION     XP_077516725.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660599.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..146
                     /product="uncharacterized protein LOC144127639"
                     /calculated_mol_wt=15820
     CDS             1..146
                     /gene="LOC144127639"
                     /coded_by="XM_077660599.1:281..721"
                     /db_xref="GeneID:144127639"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrppfa altsvlccqr pasigpcvlp afgcvippdp
       61 fmlktkscme vvqatklrvi fardleqagk ladgqwysls kasffsktkt iflgswvmyl
      121 gtcgqdnagq hasassekkh etvnky
//
LOCUS       XP_077516745             293 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127690 [Amblyomma americanum].
ACCESSION   XP_077516745
VERSION     XP_077516745.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660619.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 12% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..293
                     /product="uncharacterized protein LOC144127690"
                     /calculated_mol_wt=32513
     Region          4..97
                     /region_name="THAP"
                     /note="The THAP domain is a putative DNA-binding domain
                     (DBD) and probably also binds a zinc ion; smart00980"
                     /db_xref="CDD:214951"
     Region          <104..>157
                     /region_name="PRK06991"
                     /note="electron transport complex subunit RsxB"
                     /db_xref="CDD:235903"
     CDS             1..293
                     /gene="LOC144127690"
                     /coded_by="XM_077660619.1:110..991"
                     /db_xref="GeneID:144127690"
ORIGIN      
        1 mvncavvgcs nrsdvtgrkk kptsarffsl pkvienqcer tktlsakrrs lwlarikran
       61 fnhespnvrv cgvhfikgep snlfdetnpd wapslslghg trhadsarhd rrakrlahkr
      121 radveatava seaspracpd spqppqaeev sadenetgia vqtdmtmqdi qaleqhsvvl
      181 nehlytlqke keqlevteds lksceakvrl ytgmpnfavl favfealasf ishnvnnklt
      241 kfqefvlfmm klkvnlqnad ltfrtpsles svfgsgengm fvaviiilae ngt
//
LOCUS       XP_077516747             171 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127698 [Amblyomma americanum].
ACCESSION   XP_077516747
VERSION     XP_077516747.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660621.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..171
                     /product="uncharacterized protein LOC144127698"
                     /calculated_mol_wt=18484
     CDS             1..171
                     /gene="LOC144127698"
                     /coded_by="XM_077660621.1:12..527"
                     /db_xref="GeneID:144127698"
ORIGIN      
        1 meaattpaga dftagcpass astagdledv eeatsdptml kvlrllleir qqqweilqrl
       61 srleqarqel ktrspspvss lppqcpqlpa fniedfeaae aslrddrvaa alrkqlvcqg
      121 gnnlkevaan vmsavmgvai yaetiqpage krktpirwhk avhggnryis f
//
LOCUS       XP_077516756             186 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127727 [Amblyomma americanum].
ACCESSION   XP_077516756
VERSION     XP_077516756.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660630.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..186
                     /product="uncharacterized protein LOC144127727"
                     /calculated_mol_wt=21416
     CDS             1..186
                     /gene="LOC144127727"
                     /coded_by="XM_077660630.1:169..729"
                     /db_xref="GeneID:144127727"
ORIGIN      
        1 mamssavlim mltlhqlyfm tllmswisfh nmllsaavih yrarcqrirs isnsrtplta
       61 gwsvhevavm ygleslapps krrmwmknrs sdwwdnivcs nfddgewken frmsrvnfce
      121 lvaalapfms pecnyvrepv pvdkriaial yklasccvyr vvanqfgihk stvlkflylf
      181 citevl
//
LOCUS       XP_077516770             130 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127744 [Amblyomma americanum].
ACCESSION   XP_077516770
VERSION     XP_077516770.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660644.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 22% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..130
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..130
                     /product="uncharacterized protein LOC144127744"
                     /calculated_mol_wt=14789
     Region          <5..41
                     /region_name="MULE"
                     /note="MULE transposase domain; pfam10551"
                     /db_xref="CDD:463145"
     CDS             1..130
                     /gene="LOC144127744"
                     /coded_by="XM_077660644.1:1..393"
                     /db_xref="GeneID:144127744"
ORIGIN      
        1 mlpteapqaf mtdnsaaeka alqatwpegk qllchfhvaq aewrwlhasr dtsrddkrdl
       61 mttfqkdaae vlknitealw rqyslaadnp fyltllqrdl allkrartep qeedcaseee
      121 eqpntlvtae
//
LOCUS       XP_077516786             179 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127765 [Amblyomma americanum].
ACCESSION   XP_077516786
VERSION     XP_077516786.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660660.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 14% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..179
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..179
                     /product="uncharacterized protein LOC144127765"
                     /calculated_mol_wt=19861
     Region          <63..>98
                     /region_name="PRK09752"
                     /note="AIDA-I family autotransporter YfaL"
                     /db_xref="CDD:182059"
     CDS             1..179
                     /gene="LOC144127765"
                     /coded_by="XM_077660660.1:1..540"
                     /db_xref="GeneID:144127765"
ORIGIN      
        1 mfrsagfylv vqseritiie aseghepper nlhetsgpki dednlsrgde rgasascrqd
       61 trsptdptpv pspqpsqqht pepplqpstr atpsllgkrr rddgankvlr qmlyeshrld
      121 sltdarnrqd aafqqsmlea iwevtdavqa sngqqelliq nvnlltlilq kqleppaph
//
LOCUS       XP_077516797             308 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127773 [Amblyomma americanum].
ACCESSION   XP_077516797
VERSION     XP_077516797.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660671.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 21% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..308
                     /product="uncharacterized protein LOC144127773"
                     /calculated_mol_wt=34401
     CDS             1..308
                     /gene="LOC144127773"
                     /coded_by="XM_077660671.1:1..927"
                     /db_xref="GeneID:144127773"
ORIGIN      
        1 mkrqketwkg raqelqesel ekekeywksr aqelqddnvf lkaqvaslqr cleskifqhk
       61 nafcshvicf nmtadvlfvn lfifffctve qqgkahysrg mttglfpvgt scdaletmhg
      121 qcygalylle lalharappv aaadedlvgl aclsaateae ertsspgeeq qqqhfltppa
      181 ptgefsymed gsfyltkgiv isgtcaekif knkkptlvik dtaqaiwgnd vlvtrsvtgt
      241 acskkralge lpkqpltpek llvvsetlkh wgvqksvste eaektltrtl tkkiqdllks
      301 klrkqikf
//
LOCUS       XP_077516818             381 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127784 [Amblyomma americanum].
ACCESSION   XP_077516818
VERSION     XP_077516818.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660692.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..381
                     /product="uncharacterized protein LOC144127784"
                     /calculated_mol_wt=43057
     Region          <202..272
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(226..229,233..236)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133137"
     CDS             1..381
                     /gene="LOC144127784"
                     /coded_by="XM_077660692.1:1..1146"
                     /db_xref="GeneID:144127784"
ORIGIN      
        1 mvsevtssra kkkmtelppp eplrvganva dnwtrfkqrv elyfiatest epgrqrsnaq
       61 kaaillhlag qeaidvyntf dltaedkdyd klvqafeayc lpqsnetfer yvfrnrtqae
      121 gepfeqlyrd lqlksktcnf dnlresmlrd qivygtsskt lrekllakkd ltlttavewa
      181 kaeeitaaqs kvwsedksvd aanllprglf ealgtkqqpe paravlrsyg gneivhmgkv
      241 clpvrigadt rlveffvvkk grqailglqa ceqfglvnri ravkkdeals ecvrrqypkl
      301 ftgigklkre ysmtlqdgar pvaeparrvp hairsrlkee ldrleaagii akvrepsdwv
      361 splviarkkn gqlrvcmdpk k
//
LOCUS       XP_077516867             540 aa            linear   INV 25-NOV-2025
DEFINITION  organic cation transporter protein-like, partial [Amblyomma
            americanum].
ACCESSION   XP_077516867
VERSION     XP_077516867.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660741.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         <1..540
                     /product="organic cation transporter protein-like"
     Region          60..531
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..540
                     /gene="LOC144127805"
                     /coded_by="XM_077660741.1:<1..1623"
                     /db_xref="GeneID:144127805"
ORIGIN      
        1 atyhwrsfep grfsgfrpci klyqinysya lyiwailfpl aerqreqvlr samdildvlg
       61 gfglyhvltv flvvlrafpt awtnmmtpiv apdmdhwcar pegapssiin msadewkswa
      121 vpefanrfsk chlyrvfeda ngswtvdrnq tllcnrweyd tsvyqstivn ewnlvcgrsy
      181 hrsvaqsvvm agalvgvllf gemsdrlgrr ptfftcvlvi lvfgslssla pgigwynagr
      241 fvvamgvags qattvsllme imppqyrmmm nvgfgigyti pllllpllay yirdwgylql
      301 atglsalmmv pfwlacnyls grtpslpctr eskkatafhf vdffrtpklr rntlvlfalw
      361 fmgsfvyynl vlsstsapgn pyfnyaisaa seipaallgl wvarrcsrrr sqalflllaa
      421 laivplpflp kdspgwlrig snvlvrflal nagfikwima levfptlars fgfacvltcs
      481 rfgamvapfl rdlgeatdps vpyviqalfl llasgislll petlnkplpd tfheaesisr
//
LOCUS       XP_077516889             513 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127820 [Amblyomma americanum].
ACCESSION   XP_077516889
VERSION     XP_077516889.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660763.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 39% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..513
                     /product="uncharacterized protein LOC144127820"
                     /calculated_mol_wt=57164
     Region          <110..150
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     Region          300..387
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(315,317,319,341..343,380)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133137"
     Site            315..317
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133137"
     Site            315
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133137"
     Site            order(341..344,348..351)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133137"
     Region          460..>512
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     CDS             1..513
                     /gene="LOC144127820"
                     /coded_by="XM_077660763.1:1..1542"
                     /db_xref="GeneID:144127820"
ORIGIN      
        1 mseetkvssq pflvqppasv nfdktsewpg wieefgdyrf asglnertqe aqvrtllytm
       61 grqarnifkt fnlseedskk ydvvktqfht hfvatrnlvy esacfhrrhq epgesvdqyv
      121 telhtltdrc dygkmkermi ldrfvvglrd aklsealqmd aaltlksalt karlkeavqq
      181 qqqdlrsvvd reehesltta evdivhrrts nqrrpdhqtc aacgrgphsr sscsargalc
      241 yvcqrrghfa avcsrthrtt epeawpsevg irsprgeqln mncvtfsvgd lavssakarf
      301 vqiaingavv sakvdtgadv nvvpgsfpal pkvlqptdvi lmgpggdrln lkgkftadil
      361 wkraavrqnv fvvdsvsnvi lglpaiqalg ivkfvdeasv hqvvqtrfgs lfrglgtmkg
      421 eysirlkpda vpyaihiapr ipiplrdgvk qeleemekng virkvqgpte wcagivpvlk
      481 psrkvricvd ltqlnksvqr erfvlptvdk tlg
//
LOCUS       XP_077516897             158 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome P450 3A29-like [Amblyomma americanum].
ACCESSION   XP_077516897
VERSION     XP_077516897.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660771.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 71% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="cytochrome P450 3A29-like"
                     /calculated_mol_wt=17492
     Region          <27..137
                     /region_name="cytochrome_P450"
                     /note="cytochrome P450 (CYP) superfamily; cl41757"
                     /db_xref="CDD:477761"
     CDS             1..158
                     /gene="LOC144127824"
                     /coded_by="XM_077660771.1:1..477"
                     /db_xref="GeneID:144127824"
ORIGIN      
        1 msetkkvrhl mrgvkvqlfv gddsgreldy dqlhslsrld aiireclrly ppvvafiama
       61 csqgtkvagh eipagvsvlv pswalvekfl rrkknfpiph rrthhpasyv pfgpgprecl
      121 grrfalleln ttgfsvlsaq avqdslltvl mdasersd
//
LOCUS       XP_077516919             377 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127834 [Amblyomma americanum].
ACCESSION   XP_077516919
VERSION     XP_077516919.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660793.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 30% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..377
                     /product="uncharacterized protein LOC144127834"
                     /calculated_mol_wt=41330
     Region          <76..>211
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     CDS             1..377
                     /gene="LOC144127834"
                     /coded_by="XM_077660793.1:1..1134"
                     /db_xref="GeneID:144127834"
ORIGIN      
        1 mgfnfrrvpr vivrmltsla vivvgihmya rtadmtedth svggnqvnkl rkpdprifdl
       61 tlgppptdap aphqgslkmk dapsspetht ttqpptetrs pttessgght trttsktslg
      121 nmttekptpt rvpeqttapd rspganetas dkqgmkdppv pttnvsdsps sndkdgtvcp
      181 tvhksfcnvn gaaqffydpd egsclsatvq rahvcnrspn qfasiedcla acvkgrwape
      241 hrcvqkprfv actaedargd wwfhdgrkcv pwsfaggrcp lasapvfdta avcgaqcnds
      301 rtrrergcll pvsqlcepsq lrlpffaals vtdkgtafqc hrvewefllr hrcllgsnaf
      361 vnrdechetc rrrtrqy
//
LOCUS       XP_077516933             352 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127840 [Amblyomma americanum].
ACCESSION   XP_077516933
VERSION     XP_077516933.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660807.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..352
                     /product="uncharacterized protein LOC144127840"
                     /calculated_mol_wt=38907
     Region          103..150
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          184..343
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..352
                     /gene="LOC144127840"
                     /coded_by="XM_077660807.1:1..1059"
                     /db_xref="GeneID:144127840"
ORIGIN      
        1 mqnatgnsdh esagsgsfsv liccaqgcdv stqithretr dcevqhkpav tstfsgpdhr
       61 tsyfagydsi ekssgaledl csvncvvfal llsmlpishe rkcdvskenr lliflmklkl
      121 gisysslasl fninetsasr hfksvlktla fatkqwffrp ptrviqatmp tcfeahypgc
      181 tmiidctevr terpptiqqq ralystykgg ytlkflvgil psgaicfhsn ayggrmsdth
      241 itvdsgfldl veagdlilae kgfpgirtvl gtrnavlvmp sflqgpqfta qevrdtynva
      301 qvrihvkrmi qrikmnniln nrvpvelipl mtdvlhmccv lanlqpplik ck
//
LOCUS       XP_077516944             245 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127850 [Amblyomma americanum].
ACCESSION   XP_077516944
VERSION     XP_077516944.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660818.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 16% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..245
                     /product="uncharacterized protein LOC144127850"
                     /calculated_mol_wt=27432
     CDS             1..245
                     /gene="LOC144127850"
                     /coded_by="XM_077660818.1:1..738"
                     /db_xref="GeneID:144127850"
ORIGIN      
        1 mksnslrkta kskqqeildi dvkiqekqss raftifvlfl sqvcerhfkp fdmvnrtsyt
       61 dtktgkvieg alklvrlrpd avsrilpdcp aylpapnagt sreapdekrt rreaaalqda
      121 inlsveahqa eeernkidhi qallkcipci kvsefwtvvs qhdfilfldl tvdgapaikk
      181 svkltedlsl klyfrdvevt klegveiipk tlndircltg lldavesali rllpsanlkt
      241 tqvay
//
LOCUS       XP_077516964             275 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127864 [Amblyomma americanum].
ACCESSION   XP_077516964
VERSION     XP_077516964.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660838.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 20% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..275
                     /product="uncharacterized protein LOC144127864"
                     /calculated_mol_wt=30930
     Region          80..240
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..275
                     /gene="LOC144127864"
                     /coded_by="XM_077660838.1:1..828"
                     /db_xref="GeneID:144127864"
ORIGIN      
        1 mpfedqillt lmrlrlglll ndlslrfgas taaisrifcf ivtklakfar ecllfwllrt
       61 airrtlpyvf eenysvtciv dcfeifidrp gqlhrrntty ssyksyntak ilhvivpngf
      121 imflskpygg rasdrfitln sgfldhltqg devladrgfn iddllppsvk vslpsfskgr
      181 pqicrsdvvs srrlarlrih vdrsirrlkc frilkqlpas iftkkpland vlvaiaglcn
      241 llpnlirghg dasepgaqsp shpgrqlevv dntpe
//
LOCUS       XP_077516983             476 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127876 [Amblyomma americanum].
ACCESSION   XP_077516983
VERSION     XP_077516983.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660857.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..476
                     /product="uncharacterized protein LOC144127876"
                     /calculated_mol_wt=53126
     CDS             1..476
                     /gene="LOC144127876"
                     /coded_by="XM_077660857.1:1..1431"
                     /db_xref="GeneID:144127876"
ORIGIN      
        1 mrrafrhrlp vvttmyllgp rpfeldyngi ecrnfgrmpr avicesrgiv qrllavcalm
       61 ltkfsallpa ahldgdgkip gqftsnasrg sisescnngr nrnrfyevnl dvraqvaapl
      121 pptldyvhea tgrvdfrsfe svtqvlrvvh sakeaipves ltiivpllls lvltgwwwyk
      181 rrqsssttsa dsrtscrsns slhtlqesde akhrrpggpt lrsyrlgrrr hsvpipsvqf
      241 palanfgpae stgrdlalad alsrircdsd eppeselenv athataglst lvsdttakrm
      301 aketseddel rtapleagqa pcellmgrrl rarlpdfadr ratevkkhtq ahpqrrplea
      361 lgehdtvrll dkggwttkal aenavaprsy mvrkkdvnql rrnrqhllrt neefdpslet
      421 ipdctedsas dcaslpglrh hrspwatrap rvtikprlpt rlksilsqgn ivlwkp
//
LOCUS       XP_077516989             330 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127885 [Amblyomma americanum].
ACCESSION   XP_077516989
VERSION     XP_077516989.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660863.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 24% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..330
                     /product="uncharacterized protein LOC144127885"
                     /calculated_mol_wt=36556
     CDS             1..330
                     /gene="LOC144127885"
                     /coded_by="XM_077660863.1:1..993"
                     /db_xref="GeneID:144127885"
ORIGIN      
        1 myrsqitrvv aceqqrslgg hcllgfareq vpigrgsrcl firpaptkrl vrrgsccrpv
       61 lriagraasg tpsfagrvra aaeglqaagg crllrdpagt elqaqdfghr ytvrgydsfv
      121 dcgrltfldp lpkarvcclc hdvlpvqyla pcgnavcasc ldvsikeapa kcrerregts
      181 peghakfsmr gvtrldfnak dlgtlrvycp niwygctqss elrhleehyl kncphhpkmc
      241 fhcrredipp gdfvphiysc krrqeearsv drqsppvqqs lsesrasqte dehlcatqal
      301 laevtergge vhpepvehln qlaadikrap
//
LOCUS       XP_077516998             282 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127895 [Amblyomma americanum].
ACCESSION   XP_077516998
VERSION     XP_077516998.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660872.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..282
                     /product="uncharacterized protein LOC144127895"
                     /calculated_mol_wt=30038
     Region          <68..>198
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     CDS             1..282
                     /gene="LOC144127895"
                     /coded_by="XM_077660872.1:412..1260"
                     /db_xref="GeneID:144127895"
ORIGIN      
        1 mltslamllt lavivvgihm yartadmted tlsvggnqvn klrkpdprif dltlgppptd
       61 apaphqgslk mkdapsspet htttqpptet rspttessgg httrttskts lgnmttekpt
      121 ptrvpeqtta pdrspganet asdkqgmkdp pvpttnssnd kdgtvcptvh ksfcnvngaa
      181 qffydpdegs clsatvqrah vcnrspnqfa siedclaacv kgrwapehrc vqkprfvact
      241 aedargdwwf hdgrkcvpws faggrcplas apvfdtaavc ga
//
LOCUS       XP_077517011             260 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127902 [Amblyomma americanum].
ACCESSION   XP_077517011
VERSION     XP_077517011.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660885.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 39% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..260
                     /product="uncharacterized protein LOC144127902"
                     /calculated_mol_wt=27937
     Region          <6..>133
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     CDS             1..260
                     /gene="LOC144127902"
                     /coded_by="XM_077660885.1:33..815"
                     /db_xref="GeneID:144127902"
ORIGIN      
        1 mkdapsspet htttqpptet rspttessgg httrttskts lgnmttekpt ptrvpeqtta
       61 pdrspganet asdkqgmkdp pvpttnvsds pssndkdgtv cqtvhksfcn vngaaqffyd
      121 pdegsclsat vqrahvcnrs pnqfasiedc laacvkgrwa pehrcaqkpr fvactaedar
      181 gdwwfhdgrk cvpwsfaggr cplasapvfd taavcgaqcn dsrsrrergc llpvsqfnaq
      241 kagdrdmrrp eesapenssk
//
LOCUS       XP_077517021             356 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127908 [Amblyomma americanum].
ACCESSION   XP_077517021
VERSION     XP_077517021.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660895.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..356
                     /product="uncharacterized protein LOC144127908"
                     /calculated_mol_wt=40655
     Region          172..326
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..356
                     /gene="LOC144127908"
                     /coded_by="XM_077660895.1:26..1096"
                     /db_xref="GeneID:144127908"
ORIGIN      
        1 mvrtsilsdp yclaflswed ieavmmrere qqrrlyasrr gllnldtmpd stfrshfrfq
       61 kadfrtlvna levpeyvtsa qgvrvsgrea lciclrrlay pnrlcdlqef fgrhysviss
      121 isnktmlhie qrfgslldnl lchkwlstsy lkemsnavhl kgapldncwa fidgtarpic
      181 rpsegqrvyf sghkrvhalk yqalmcpngl icqldgpypg srhdagilre sklyetleel
      241 vpgndaviyg dpayplrpll mkpyggarlt taqenfnaam stvrqavewg fgrvisqfaf
      301 ldfkknqrll lqavprmyrv aviltnchac mygnqvsnyf dleppclfdy lhpnqs
//
LOCUS       XP_077517040             679 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127919 [Amblyomma americanum].
ACCESSION   XP_077517040
VERSION     XP_077517040.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660914.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..679
                     /product="uncharacterized protein LOC144127919"
                     /calculated_mol_wt=74837
     CDS             1..679
                     /gene="LOC144127919"
                     /coded_by="XM_077660914.1:1..2040"
                     /db_xref="GeneID:144127919"
ORIGIN      
        1 millltcvgt llaaifvgly hkpwpfvllg iwhrqaedvp glrrltayhd ppqvaaplpp
       61 tldyvheatg rvdflsfesv tqalrvvhsa keaipveslt iivplllslv ltgwwwykrr
      121 qsssttsads rtscrsnssl htlqesdeak hrrpggptlr syrlgrrrhs vpipsmqfpa
      181 lanfgpaest gtddsasvpv lrqrspltgf ltaseeqlfp lsvssdtdqp lnvggrlkrw
      241 lrdavwpdll gynpticggv ledfsaaeyh ggtlrteela prpqtssygg ctrddvnrad
      301 danrasteaf etpllddiyc rrsdasedgt detevvegnw adldesrpqr lewqpshhhq
      361 rderrrsgla dwelshvnns glsedtayla lpenkcpsee vlgacsfdqh qqnawcaaaa
      421 avapcfvspv eplraprhsq dvfeqllkac rqpeavafse ilqelncrpk isdigtpcgd
      481 ttpsstaags lslihcpkrg cvacattccr ssillraamp yapaawtfps krpppsagkd
      541 gkalrpkavp ssacvasrdl tstprtwsse lrhleehylk ncphhpkmcf hcrredippg
      601 dfvphiysck rrqeearsvd rqsppvqqsl sespasqted ehlcatqall aevterggev
      661 hpepvehlnq laadikrap
//
LOCUS       XP_077517053             714 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127922 [Amblyomma americanum].
ACCESSION   XP_077517053
VERSION     XP_077517053.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660927.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 44% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..714
                     /product="uncharacterized protein LOC144127922"
                     /calculated_mol_wt=79602
     CDS             1..714
                     /gene="LOC144127922"
                     /coded_by="XM_077660927.1:9..2153"
                     /db_xref="GeneID:144127922"
ORIGIN      
        1 millltcvgt llaaifvgly hkpwpfvllg iwhrqaedvp glrrltayhd ppqvaaplpp
       61 tldyvheatg rvdfrsfesv tqalrvvhsa keaipveslt iivplllslv ltgwwwykrr
      121 qsssttpads rtscrsnssl htlqesdeak hrrpggptlr syrlgrrrhs vpipsvqfpa
      181 lanfgpaest grdlaladal srircdsdep peselenvat hataglstlv sdttakrmak
      241 etseddelrt apleagqalc ellmgrrlra rlpdfadrra pevkkhtqah pqrrplealg
      301 ehdtvrlldk ggwttkalae navaprsymv rkkdvnqlrr nrqhllrtne efdpsletip
      361 dctedsasdc aslpglrhhr spwatraprv tikprlptrl ksilsqvsvs sdtdqplnvg
      421 grlkrwlrda vwpdllgynp ticggvledf saaeyhggtl rteelaprpq tssyggctrd
      481 dvnraddanr asteafetpl lddiycrrsd asedgtdete vvegnwadld esrpqrlewq
      541 psrhhqrder rrsgladwel shvnnsglse dtaylalpen kcpseevlga csfdqhqqna
      601 wcaaaaavap cfvspveplr aprhsqdvfe qllkacrqpe avafseilqe lnapklrtpr
      661 sfvdrwrrha sqepacaslf qsscttarts agqrrfpaaw rsmgeqssss wdvc
//
LOCUS       XP_077517064             403 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127929 [Amblyomma americanum].
ACCESSION   XP_077517064
VERSION     XP_077517064.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660938.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..403
                     /product="uncharacterized protein LOC144127929"
                     /calculated_mol_wt=43846
     Region          343..>378
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     CDS             1..403
                     /gene="LOC144127929"
                     /coded_by="XM_077660938.1:1..1212"
                     /db_xref="GeneID:144127929"
ORIGIN      
        1 mallalgrpt lrsyrlgrrr hsvpipsvqf palanfgpae stgtddsasv pvlrqrsplt
       61 gfltaseeql fplsvssdtd qplnvggrlk rwlrdavwpd llgynpticg gvledfsaae
      121 yhggtlrtee laprpqtssy ggctrddvnr addanraste afetpllddi ycrrsdased
      181 gtdetevveg nwadldesrp qrlewqpshh hqrderrrsg ladwelshvn nsglsedtay
      241 lalpenkcps eevlgacsfd qhqqnawcaa aaavapcfvs pveplrapps fagrvraaae
      301 glqaaggcrl lrdpagtqcs qaahteklrg qmakarvara rmrfavpvil ydgknicrsa
      361 tlsggleiyg rtiikflgcm ltgrlessev dvthslcgwk ipc
//
LOCUS       XP_077517078             379 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127934 [Amblyomma americanum].
ACCESSION   XP_077517078
VERSION     XP_077517078.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660952.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..379
                     /product="uncharacterized protein LOC144127934"
                     /calculated_mol_wt=41408
     Region          <78..>212
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     CDS             1..379
                     /gene="LOC144127934"
                     /coded_by="XM_077660952.1:1..1140"
                     /db_xref="GeneID:144127934"
ORIGIN      
        1 mdavprvilr mltslamllt lavivfgihm yartadmted tlsvggnqvn klrkpdprif
       61 dltlgppptd apaphqgslk mkdapsspet htttqpptet rspttessgg httrttskts
      121 lgnmttekpt ptrvpeqtta pdrspganet asdkqgmkdp pvpttnvsds pssndkdgtv
      181 cptvhksfcn vngaaqffyd pdegsclsat vqmahvcnrs pnqfasiedc laacvkgrwa
      241 pehrcvqkpr fvactaedar gdwwfhdgrk cvpwsfaggr cplasapvfd taavcgaqcn
      301 dsrtrrergc llpvsqlcep sqlrlpffaa psvtdkgtaf qchrvewefl lrhrcllgsn
      361 afvnrdeche tcrrrmrqy
//
LOCUS       XP_077517097             432 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127950 [Amblyomma americanum].
ACCESSION   XP_077517097
VERSION     XP_077517097.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660971.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..432
                     /product="uncharacterized protein LOC144127950"
                     /calculated_mol_wt=48082
     CDS             1..432
                     /gene="LOC144127950"
                     /coded_by="XM_077660971.1:231..1529"
                     /db_xref="GeneID:144127950"
ORIGIN      
        1 mgtyasiaaa lhvvyhmkec lppgslmiiv pvllsliltr rrrtqessrp aspmacctfv
       61 tpsrddvrwd rsrteedaai gpplvptekr eledsrraqr cslhkldrhr prrnkkeptr
      121 tgssgnhgas gcqeqrrpqs apagewlasc degkdsrlps tdalgngfhg rpghgssasq
      181 esvevarrqc ddddeekvli tdlswnsfdv tdeshpsgta dswpmgikth fpermsampa
      241 ephavaeadg eelsdrhcrd hcltavakpe vgttaetfgg reldellqlc gqkrpatfsd
      301 llqelgvdkc vklrerdgee ifrvstgrgd slltvlhddy ltrhwkrtld dimahsgpsp
      361 evtadsfnnd agwddirkcy cvrdkysevl nkacinyfyl kgctkfgede ramilpymvv
      421 lkplpgqtsn yl
//
LOCUS       XP_077517105             489 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127957 [Amblyomma americanum].
ACCESSION   XP_077517105
VERSION     XP_077517105.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660979.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..489
                     /product="uncharacterized protein LOC144127957"
                     /calculated_mol_wt=55456
     Region          141..263
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(159..160,214..219,230..232,253..256)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Site            order(159..160,215..219,255)
                     /site_type="other"
                     /note="SAM binding site [polypeptide binding]"
                     /db_xref="CDD:380914"
     Region          290..313
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          321..341
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          325..>401
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Site            order(326,328,330,332..333,336..337,340,354,356,360..361,
                     364..365,368,382,384,386,388..389,392..393,397)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          349..369
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          361..386
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          377..398
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          406..422
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..489
                     /gene="LOC144127957"
                     /coded_by="XM_077660979.1:1..1470"
                     /db_xref="GeneID:144127957"
ORIGIN      
        1 meteeyhclk pvyfsedtwh kppddikkcy gnvkkdgslk ekkgvstgde epmvvepvev
       61 irpqvsqsec ppapeymlld hwealgsdgp dqpeepasre ehrdetpfdg sgmvaradst
      121 sgtmladvks vvnsedasfv gmsifeflts gkstitgvpe gvftlkalpk glhfgpyhrv
      181 kvdgienggc twpvrrcaef fvvdgrqqeh nhwmnnvnys pskrrqnlva llfngdiyyr
      241 tlrnvgpgee llvgystsft esllgnprgr galkeqdvni vplhslflac ekcgdlfttq
      301 ynlemhtrrk hwhstegihh cthctytstr kvdvfrhert hvarresvch lcqkrfsqpd
      361 nlkvhlmiht gekpyecsqc gkrfrqrvha rrhehvihsr qyplhcprcg agaedvtrlr
      421 rhtckplgtt dskeelkwgr grprlradgt apkvrprntt egtpqqgrvk rgrgrprkav
      481 kqdvspprp
//
LOCUS       XP_077517114             153 aa            linear   INV 25-NOV-2025
DEFINITION  histone-lysine N-methyltransferase PRDM9-like [Amblyomma
            americanum].
ACCESSION   XP_077517114
VERSION     XP_077517114.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077660988.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..153
                     /product="histone-lysine N-methyltransferase PRDM9-like"
                     /calculated_mol_wt=16753
     Region          10..128
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(24..25,79..84,95..97,118..121)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Site            order(24..25,80..84,120)
                     /site_type="other"
                     /note="SAM binding site [polypeptide binding]"
                     /db_xref="CDD:380914"
     CDS             1..153
                     /gene="LOC144127964"
                     /coded_by="XM_077660988.1:1..462"
                     /db_xref="GeneID:144127964"
ORIGIN      
        1 mpvssvracp elltlgkstv ngcpvgvftl kalpkglhfg pyqgvkvddi enggctwpvr
       61 rcgeffvvdg rpqqrnhwmn dvnyspskrr hnlvalfvng diyyrtlknv gpgeemlvry
      121 stsfaksllg nprepgalng geeklilhsl aar
//
LOCUS       XP_077517127             360 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127972 [Amblyomma americanum].
ACCESSION   XP_077517127
VERSION     XP_077517127.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661001.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..360
                     /product="uncharacterized protein LOC144127972"
                     /calculated_mol_wt=40740
     Region          218..241
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          249..269
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(254,256,258,260..261,264..265,268,282,284,288..289,
                     292..293,296,310,312,314,316..317,320..321,325)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          277..297
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          282..>339
                     /region_name="transpos_IS66"
                     /note="IS66 family transposase; NF033517"
                     /db_xref="CDD:468053"
     Region          291..312
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          305..326
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          334..350
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..360
                     /gene="LOC144127972"
                     /coded_by="XM_077661001.1:94..1176"
                     /db_xref="GeneID:144127972"
ORIGIN      
        1 mhffqgvase edepmvvepn eetqserppa peyilleqwe aygsggpylp eepasweeqr
       61 dkkaydgrei varadaalde vmreaieafg ethresppst spdalpdhwe apgsggpnlp
      121 eeppsreeqh ednpidgsgt fasvytalne aekeasdafg dthresppst spdalpdhwe
      181 apgsgdqhll eepssreeqh dknqdvntip lhslflacek csdlftkqyn lemhtrrkhw
      241 hstegihhcs hctytstrka diirherthv grrdsvchlc qkrfsrpdhq kvhmiihsge
      301 kpyecsqcgk mfreraharr heqvihsrry pltcprcgag aedvtrlrrh tckplgttdg
//
LOCUS       XP_077517137             449 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127979 [Amblyomma americanum].
ACCESSION   XP_077517137
VERSION     XP_077517137.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661011.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 11% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..449
                     /product="uncharacterized protein LOC144127979"
                     /calculated_mol_wt=50178
     CDS             1..449
                     /gene="LOC144127979"
                     /coded_by="XM_077661011.1:1..1350"
                     /db_xref="GeneID:144127979"
ORIGIN      
        1 mrrafrhrlp vvttmyllgp rpfeldyngi ecrnfgrmpr avicesrgig kipgqftsna
       61 srgsisescn ngrnrnrfye vnldvraqva aplpptldyv heatgrvdfr sfesvtqalr
      121 vvhstkeais vesltiivpl llslvltgww wykrrqssst tsadsrtscr snsslhtlqe
      181 sdeakhrrpg gptlrsyrlg rrrhsvpips vqfpalanfg paestgrdla lagalsrirc
      241 dsdeppesel envathatag lstlvsdtta krmaketsed delrtaplea gqapcellmg
      301 rrlrarlpdf adrrapevkk htqahpqrrp lealgehdtv rlldkggwtt kalaenaaap
      361 rsymvrkkdv nqlrrnrqhl lrtneefdps letipdcted sasdcaslpg lrhhrspwat
      421 raprvtikpr lptrlksils qgnivlwkp
//
LOCUS       XP_077517151             414 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127987 [Amblyomma americanum].
ACCESSION   XP_077517151
VERSION     XP_077517151.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661025.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..414
                     /product="uncharacterized protein LOC144127987"
                     /calculated_mol_wt=46156
     CDS             1..414
                     /gene="LOC144127987"
                     /coded_by="XM_077661025.1:9..1253"
                     /db_xref="GeneID:144127987"
ORIGIN      
        1 millltcvgt llaaifvgly hkpwpfvllg iwhrqaedvp glrrltayhd ppqvaaplpp
       61 tldyvheatg rvdfrsfesv tqalrvvhsa keaipveslt iivplllslv ltgwwwykrr
      121 qsssttpads rtscrsnssl htlqesdeak hrrpggptlr syrlgrrrhs vpipsvqfpa
      181 lanfgpaest grdlaladal srircdsdep peselenvat hataglstlv sdttakrmak
      241 etseddelrt apleagqapc ellmgrrlra rlpdfadrra pevkkhtqah pqrrplealg
      301 ehdtvrlldk ggwttkalae navaprsymv rkkdvnqlrr nrqhllrtne efdpsletip
      361 dctedsasdc aslpglrhhr spwasraprv tikprlptrl ksilsqgniv lwkp
//
LOCUS       XP_077517163             373 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127992 [Amblyomma americanum].
ACCESSION   XP_077517163
VERSION     XP_077517163.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661037.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 36% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..373
                     /product="uncharacterized protein LOC144127992"
                     /calculated_mol_wt=41509
     CDS             1..373
                     /gene="LOC144127992"
                     /coded_by="XM_077661037.1:1..1122"
                     /db_xref="GeneID:144127992"
ORIGIN      
        1 millltcvgt llaaifvgly lkpwpfvllg iwhrqaedvp glrrltayhd ppqvaaplpp
       61 tldyvheatg rvdfrsfesv tqalrvvhsa keaipveslt iivplllslv ltgwwwykrr
      121 qsssttpads rtscrsnssl htlqesdeak hrrpggptlr syrlgrrrhs vpipsvqfpa
      181 lanfgpaest gtddsasvpv lrqrspltgf ltaseeqlfp lsvssdtdqp lnvggrlkrw
      241 lrdavwpdll gynpticggv ledfsadeyy ggtlrteela prpqtssygg ctrddvnrad
      301 danrasteaf etpllddiyc rrsdasedgt detevvegnw adldesrpqr lewqpshhhq
      361 rderrrsgvs rlg
//
LOCUS       XP_077517170             284 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144127996 [Amblyomma americanum].
ACCESSION   XP_077517170
VERSION     XP_077517170.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661044.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..284
                     /product="uncharacterized protein LOC144127996"
                     /calculated_mol_wt=31442
     CDS             1..284
                     /gene="LOC144127996"
                     /coded_by="XM_077661044.1:1..855"
                     /db_xref="GeneID:144127996"
ORIGIN      
        1 mdavprvivr mltslamllt lavivvgihm yartadmted tlsvggnqvn klrkpdprif
       61 dltlgppptd apaphqssnd kdgtvcptvr ksfcnvngaa qffydpdegs clsatvqrah
      121 vcnrspnqfa siedclaacv kgrwapehrc vqkprfvact aedargdwwf hdgrkcvpws
      181 faggrcplas apvfdtaavc gaqcndsrtr rergcllpvs qlcepsqlrl pffaapsvtd
      241 kgtafqchrv ewefllrhrc llgsnafvnr dechetcrrr trqy
//
LOCUS       XP_077517181             379 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128006 [Amblyomma americanum].
ACCESSION   XP_077517181
VERSION     XP_077517181.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661055.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 21% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..379
                     /product="uncharacterized protein LOC144128006"
                     /calculated_mol_wt=41300
     Region          <78..>212
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     CDS             1..379
                     /gene="LOC144128006"
                     /coded_by="XM_077661055.1:1..1140"
                     /db_xref="GeneID:144128006"
ORIGIN      
        1 mdavprvivr mltslamllt lavtvvgihm yarttdmted tlsvggnqvn klrkpdprif
       61 dltlgppptd apaphqgslk mkdapsspet htttqpptet rspttessgg httrttskts
      121 lgnmttekpt ptrvpeqtta pdrspganet asdkqgmkdp pvpttnvsds pssndkdgtv
      181 cptvhksfcn vngaaqffyd pdegsclsat vqmahvcnrs pnqfasiedc laacvkgrwa
      241 pehrcvqkpr fvactaedar gdwwfhdgrk cvpwsfaggr cplasapvfd taavcgaqcn
      301 dsrtrrergc llpvsqlcep sqlrlpifaa psvtdkgtaf qchrvewefl lrhrcllgsn
      361 afvnrdeche tcrrrtrqy
//
LOCUS       XP_077517193             362 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128015 [Amblyomma americanum].
ACCESSION   XP_077517193
VERSION     XP_077517193.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661067.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 12% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..362
                     /product="uncharacterized protein LOC144128015"
                     /calculated_mol_wt=40250
     CDS             1..362
                     /gene="LOC144128015"
                     /coded_by="XM_077661067.1:1..1089"
                     /db_xref="GeneID:144128015"
ORIGIN      
        1 mssqcgrhrs wwwykrrqss sttsadsrts crsnsslhtl qesdeakhrr pggptlrsyr
       61 lgrrrhsvpi psvqfpalan fgpaestgtd dsasvpvlrq rspltgflta seeqlfplsv
      121 ssdtdqplnv ggrlkrwlrd avwpdllgyn pticggvled fsaaeyhggt lrteelaprp
      181 qtssyggctr ddvnraddan rasteafetp llddiycrrs dasedgtdet evvegnwadl
      241 desrpqrlew qpshhhqrde rrrsgladwe lshvnnsgls edtaylalpe nkcpseevlg
      301 acsfdqhqqn awcaaaaava pcfvspvepl raprhsqdvf eqllkacrqp eavtfseilq
      361 el
//
LOCUS       XP_077517204             380 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128021 [Amblyomma americanum].
ACCESSION   XP_077517204
VERSION     XP_077517204.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661078.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..380
                     /product="uncharacterized protein LOC144128021"
                     /calculated_mol_wt=41492
     Region          <78..>213
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     CDS             1..380
                     /gene="LOC144128021"
                     /coded_by="XM_077661078.1:1..1143"
                     /db_xref="GeneID:144128021"
ORIGIN      
        1 mdavprvivr mltslamllt savivvgihm yarttdmted tlsvggnqvn klrkpdprif
       61 dltlgppptd apaphqgslk mkdapsspet htttqpptet rspttessgg httrttskts
      121 lgnmttekpt ptrvpeqtta pdrspganet asdkqgmkdp pvpttnvsds pssndkdgtv
      181 cptvhksfcn vngaaqffyd pdegsclsat vqrahvcnrs pnqfasiedc laacvkgrwa
      241 pehrcvqkpr fvactaedar gdwwfhdgrk cvpwsfaggr cplasapvfd taavcgaqcn
      301 dsrtrrergc llpvsqlcep sqlrlpfffa apsvtdkgta fqchrvewef llrhrcllgs
      361 nafvnrdech etcrrrtrqy
//
LOCUS       XP_077517214             437 aa            linear   INV 25-NOV-2025
DEFINITION  PR domain zinc finger protein 5-like [Amblyomma americanum].
ACCESSION   XP_077517214
VERSION     XP_077517214.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661088.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 38% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..437
                     /product="PR domain zinc finger protein 5-like"
                     /calculated_mol_wt=49437
     Region          89..211
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(107..108,162..167,178..180,201..204)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Site            order(107..108,163..167,203)
                     /site_type="other"
                     /note="SAM binding site [polypeptide binding]"
                     /db_xref="CDD:380914"
     Region          238..261
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          269..289
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          273..>349
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Site            order(274,276,278,280..281,284..285,288,302,304,308..309,
                     312..313,316,330,332,334,336..337,340..341,345)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          297..317
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          309..334
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          325..346
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          354..370
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..437
                     /gene="LOC144128036"
                     /coded_by="XM_077661088.1:150..1463"
                     /db_xref="GeneID:144128036"
ORIGIN      
        1 mvvepvevir pqvsqsecpp apeymlldhw ealgsdgpdq peepasreeh rdetpfdgsg
       61 mvaradstsg tmladvksev nsedasfvgm sifefltsgk stitgvpggv ftlkalpkgl
      121 hfgpyhrvkv dgienggctw pvrrcaeffv vdgrqqehnh wmnnvnysps krrqnlvall
      181 fngdiyyrtl rnvgpgeell vgystsftes llgnprgrga lkeqdvnivp lhslflacek
      241 cgdlfttqyn lemhtrrkhw hstegihhct hctytsthkv dvfrherthv arreslchlc
      301 qkrfsqpdnl kvhlmihtge kpyecsqcgk rfrqrvharr hehvihsrqy plhcprcgag
      361 aedvtrlrrh tckplgttds keelkrdrgr prlradgtap kvrprntteg tpqqgrvkrg
      421 rgrprkavkq dvspprp
//
LOCUS       XP_077517220             139 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128046 [Amblyomma americanum].
ACCESSION   XP_077517220
VERSION     XP_077517220.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661094.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..139
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..139
                     /product="uncharacterized protein LOC144128046"
                     /calculated_mol_wt=15270
     CDS             1..139
                     /gene="LOC144128046"
                     /coded_by="XM_077661094.1:88..507"
                     /db_xref="GeneID:144128046"
ORIGIN      
        1 matngvasrq deakavepne etqsgrppap eyilleqwea hgsggpyfpe nsasceeqrd
       61 ekpfdgreiv aradaalddv mreaieafge tlrkslpwss pdalpdhwea pgsgdpnlpe
      121 epssrevqhd enrectird
//
LOCUS       XP_077517234             489 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128053 [Amblyomma americanum].
ACCESSION   XP_077517234
VERSION     XP_077517234.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661108.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 37% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..489
                     /product="uncharacterized protein LOC144128053"
                     /calculated_mol_wt=55525
     Region          141..263
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(159..160,214..219,230..232,253..256)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Site            order(159..160,215..219,255)
                     /site_type="other"
                     /note="SAM binding site [polypeptide binding]"
                     /db_xref="CDD:380914"
     Region          290..313
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          321..341
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          325..>401
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Site            order(326,328,330,332..333,336..337,340,354,356,360..361,
                     364..365,368,382,384,386,388..389,392..393,397)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          349..369
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          361..386
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          377..398
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          406..422
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..489
                     /gene="LOC144128053"
                     /coded_by="XM_077661108.1:1..1470"
                     /db_xref="GeneID:144128053"
ORIGIN      
        1 meteeyhclk pvyfsedtwh klpddikkcy gnvkkdgslk ekkgvstgde epmvvepvev
       61 irpqvsqsec ppapeymlld hwealgsdgp dqpeepasre ehrdetpfdg sgmvaradst
      121 sgtmladvks evnsedasfv gmsifeflts gkstitgvpe gvftlkalpk glhfgpyhrv
      181 kvdgienggc twpvrrcaef fvvdgrqqeh nhwmnnvnys pskrrqnlva llfngdiyyr
      241 tlrnvgpgee llvgystsft esllgnprgr galkeqdvni vplhslflac ekcgdlfttq
      301 ynlemhtrrk hwhstegihh cthctytsth kvdvfrhert hvarreslch lcqkrfsqpd
      361 nlkvhlmiht gekpyecsqc gkrfrqrvha rrhehvihsr qyplhcprcg agaedvtrlr
      421 rhtckplgtt dskeelkrdr grprlradgt apkvrprntt egtpqqgrvk rgrgrprkav
      481 kqdvspprp
//
LOCUS       XP_077517243             163 aa            linear   INV 25-NOV-2025
DEFINITION  histone-lysine N-methyltransferase PRDM9-like [Amblyomma
            americanum].
ACCESSION   XP_077517243
VERSION     XP_077517243.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661117.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 23% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..163
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..163
                     /product="histone-lysine N-methyltransferase PRDM9-like"
                     /calculated_mol_wt=18492
     Region          1..104
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(1,55..60,71..73,94..97)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Site            order(1,56..60,96)
                     /site_type="other"
                     /note="SAM binding site [polypeptide binding]"
                     /db_xref="CDD:380914"
     CDS             1..163
                     /gene="LOC144128060"
                     /coded_by="XM_077661117.1:1..492"
                     /db_xref="GeneID:144128060"
ORIGIN      
        1 mgvftlkalp kglhfgpyhg vkvdcieygg ctwpvrrcae ffvvdgrqqe ryhwmnnvny
       61 spskrrqnlv avlfngniyy rtlknvgpge ellvgystsf aesllgnprg rgalkeedvn
      121 ivplhslfla cencgdlfst qhnlemhtrr khwhsthgil rct
//
LOCUS       XP_077517248             633 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128066 [Amblyomma americanum].
ACCESSION   XP_077517248
VERSION     XP_077517248.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661122.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..633
                     /product="uncharacterized protein LOC144128066"
                     /calculated_mol_wt=69375
     Region          <17..>152
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     CDS             1..633
                     /gene="LOC144128066"
                     /coded_by="XM_077661122.1:1..1902"
                     /db_xref="GeneID:144128066"
ORIGIN      
        1 mtlgppptda paphqgslkm kdapsspeth tttqpptetr spttessggh ttrttsktsl
       61 gnmttekptp trvpeqttap drspganeta sdkqgmkdpp vpttnvsdsp ssndkdgtvc
      121 ptvhksfcnv ngaaqffydp degsclsatv qrahvcnrsp nqfasiedcl aacvkgrwap
      181 ehrcvqkprf vactaedarg dwwfhdgrkc vpwsfaggrc plasapvfdt aaigkilgqf
      241 tsnasrgsis escnngrnrn rfyevnldvr aqvaaplppt ldyvheatgr vdfrsfesvt
      301 qalrvvhsak eaipveslti ivplllslvl tgwwwykrrq sssttsadsr tscrsnsslh
      361 tlqesdeakh rrpggptlrs yrlgrrrhsv pipsvqfpal anfgpaestg rdlalagals
      421 rircdsdepp eielenvath ataglstlvs dttakrmake tseddelrta pleagqapce
      481 llmnrrlrar lpdfadrrap evkkhtqahp qrrplealge hdtvrlldkg gwttkalaen
      541 avaprsymvr kkdvnqlrrn rqhllrtnee fdpsletipd ctedsasdca slpglrhhrs
      601 pwatraprvt ikqrlptrlk silsqgnivl wkp
//
LOCUS       XP_077517255             746 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128072 [Amblyomma americanum].
ACCESSION   XP_077517255
VERSION     XP_077517255.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661129.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 39% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..746
                     /product="uncharacterized protein LOC144128072"
                     /calculated_mol_wt=83159
     Region          552..588
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(553,556,568,570,573,576,588)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438301"
     CDS             1..746
                     /gene="LOC144128072"
                     /coded_by="XM_077661129.1:9..2249"
                     /db_xref="GeneID:144128072"
ORIGIN      
        1 millltcvgt llaaifvgly hkpwpfvllg iwhrqaedvp glrrltayhd ppqvaaplpp
       61 tldyvheatg rvdfrsfesv tqalrvvhsa keaipveslt iivplllslv ltgwwwykrr
      121 qsssttsads rtscrsnssl htlqesdeak hrrpggptlr syrlgrrrhs vpipsvqfpa
      181 lanfgpaest grdlaladal srircdsdep peselenvat hataglstlv sdttakrmak
      241 etseddeprt apleagqapc ellmgrrlra rlpdfadrlr hhrspwatra prvtikprlp
      301 trlksilsqv svssdtdqpl nvggrlkrwl wdavwpdllg ynpticggvl edfsaaeyhg
      361 gtlrteelap rpqtssyggc trddvnradd anrasteafe tpllddiycr rsdasedgtd
      421 etevvegnwa dldesrpqrl ewqpshhrqr derrrsglad welshvnnsg lsedtaylal
      481 penkcpseev lgrvraaaeg lqaaggcrll rdpagtelqa qdfghrytvr gydsfvdcrr
      541 ltfldplpka rvcclchdvl pvqylapcgn avcascldvs ikeapakcre rregtspegh
      601 akfsmrgvtr ldfnakdlgt lrvycpniwy gctqsselrh leehylkncp hhpkmcfhcr
      661 redippgdfv phiysckrrq eearsvdrqs ppvqqslses pasqtedehl catqallaev
      721 terggeahpe pvehlnqlaa dikrap
//
LOCUS       XP_077517267             210 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128078 [Amblyomma americanum].
ACCESSION   XP_077517267
VERSION     XP_077517267.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661141.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..210
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..210
                     /product="uncharacterized protein LOC144128078"
                     /calculated_mol_wt=23536
     CDS             1..210
                     /gene="LOC144128078"
                     /coded_by="XM_077661141.1:1..633"
                     /db_xref="GeneID:144128078"
ORIGIN      
        1 mlssndkdgt vcptvhksfc nvnraaqffy dpdegsclsa tvqrahvcnr spnqfasied
       61 claacvkgrw apehrcvqkp rfvactaeda rgdwwfhdgr kcvpwsfagg rcplasapvf
      121 dtaavcgaqc ndsrtrrerg cllpvsqlce psqlrlpffa apsvtdkgta fqchrvewef
      181 llrhrcllgs nafvnrdech etcrrrtrqy
//
LOCUS       XP_077517282             681 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128083 [Amblyomma americanum].
ACCESSION   XP_077517282
VERSION     XP_077517282.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661156.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 33% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..681
                     /product="uncharacterized protein LOC144128083"
                     /calculated_mol_wt=75656
     CDS             1..681
                     /gene="LOC144128083"
                     /coded_by="XM_077661156.1:9..2054"
                     /db_xref="GeneID:144128083"
ORIGIN      
        1 millltcvgt llaaifvgly hkpwpfvllg iwhrqaedvp glrrltayhd ppqvaaplpp
       61 tldyvheatg rvdfrsfesv tqalrvvhsa keaipveslt iivplllslv ltgwwwykrr
      121 qsssttsads rtscrsnssl htlqesdeak hrrpggptlr syrlgrrrhs vpipsvqfpa
      181 lanfgpaest grdlaladal srircdsdep peselenvan hataglstlv sdttakrmak
      241 etsvddelrt apleagqapc ellmgrrlrs rlpdfadrra pevkkhtqah pqrrplealg
      301 ehdtvrlldk ggwttkalae navaprsymv rkkdvnqlrr nrqhllrtne efdpsletip
      361 dctedsasdc aslpglrhhr spwatraprv tikprlptrl ksilsqgtdd sasvpvlrqr
      421 spltgfltas eeqlfplsvs sdtdqplnvg grlkrwlrda vwpdllgynp ticggvledf
      481 saaeyhggtl rteelaprpq tssyggctrd dvnraddanr asteafetpl lddiycrrsd
      541 asedgtdete vvegnwadld esrpqrlewq pshhhqrder rrsgladwel shvnnsglse
      601 dtaylalpen kcpseevlga csfdqhqqna wcaaaaavap cfvspveplr aprhsqdvle
      661 qllkacrqte avafseilqe l
//
LOCUS       XP_077517291             358 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128088 [Amblyomma americanum].
ACCESSION   XP_077517291
VERSION     XP_077517291.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661165.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 32% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..358
                     /product="uncharacterized protein LOC144128088"
                     /calculated_mol_wt=39662
     CDS             1..358
                     /gene="LOC144128088"
                     /coded_by="XM_077661165.1:231..1307"
                     /db_xref="GeneID:144128088"
ORIGIN      
        1 mgtyasiaaa lhvvyhmkec lppgslmiiv pvllsliltr rrrtqessrp aspmacctfv
       61 tpsrddvrwd rsrteedaai gpplvptekr eledsrraqr cslhkldrhr prrnkkeptr
      121 tgssgnhgas gcqeqrrpqs apagewlasc degkdsrlps tealgngfhg rpghgssasq
      181 esvevarrqc ddddeekvli tdlswnsfdv tdeshpsgta dswpmgikth fpermsampa
      241 ephavaeadg eelsdrhcrd hcltavakpe vgttaetfgg reldellqlc gqkrqatfsd
      301 llqelgvdkc vklrerdgee ifrvskgrgd slltvlhddy ltrhwkrtld dimahsgp
//
LOCUS       XP_077517304             365 aa            linear   INV 25-NOV-2025
DEFINITION  PR domain zinc finger protein 14-like [Amblyomma americanum].
ACCESSION   XP_077517304
VERSION     XP_077517304.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661178.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..365
                     /product="PR domain zinc finger protein 14-like"
                     /calculated_mol_wt=41262
     Region          19..139
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(35..36,90..95,106..108,129..132)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Site            order(35..36,91..95,131)
                     /site_type="other"
                     /note="SAM binding site [polypeptide binding]"
                     /db_xref="CDD:380914"
     Region          166..189
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(206,208..209,212..213,216,230,232,236..237,240..241,
                     244,258,260,262,264..265,268..269)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          225..245
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          225..245
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          237..262
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          253..269
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..365
                     /gene="LOC144128094"
                     /coded_by="XM_077661178.1:1..1098"
                     /db_xref="GeneID:144128094"
ORIGIN      
        1 mfvepnevrs qdaslvgesl pelltlgkst vngcpvgvft lkalpkglhf gpyqgvkvdd
       61 ienggctwpv rrcgeffvvd grpqqrnhwm ndvtywpskr rqnlvallvn gdiyyrtlkn
      121 vgpgeellvg ystsfaesll gnprepgaln gedvnivplh sllhacekcg dlfstqykle
      181 thtrrkhwhk tegirrgthc pnksshkadi irharthvdk rksmchvckk rfskpdhlkv
      241 hmmihsgekp yecgqcgkrf rqrvharrhe evihfrrypl tcqrcgagae dvfklrhhtc
      301 kplgttedke glkrcrgrpr lredgtapkv rpqnttegtp lqggvkrgrg rprkevtqda
      361 sapra
//
LOCUS       XP_077517319             516 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128111 [Amblyomma americanum].
ACCESSION   XP_077517319
VERSION     XP_077517319.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661193.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 34% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..516
                     /product="uncharacterized protein LOC144128111"
                     /calculated_mol_wt=58207
     Region          <36..152
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            40
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238827"
     Region          317..338
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          <326..428
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          348..368
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(353,355,357,359..360,363..364,367,381,383,387..388,
                     391..392,395,409,411,413,415..416,419..420,424)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          376..396
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          404..425
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          433..449
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..516
                     /gene="LOC144128111"
                     /coded_by="XM_077661193.1:1..1551"
                     /db_xref="GeneID:144128111"
ORIGIN      
        1 mwpkdirlhk yflsnrttti rvgeeksdtv elgdrgtlqg svlspllfnl allplpdlln
       61 qiesvdhsfy tgditvwtiq agsdawteka lqraattvhe yakssglscs pqqselvmiq
      121 pgklkelppi miitidgtvi kptqqirvlg llhqidrkah aaitriktrt erilsvasrq
      181 deakavepne etqsgrppap eyilleqwea hgsggpyfpe nsasceeqrd ekpfdgreiv
      241 aradaalddv mreaieafge tlrkslpwss pdalpdhwea pgsgdpnlpe epssrevqhd
      301 enqdiniipl hslflacekc gdlftkqynm emhtrwkhwn smegihncth csytstrkvd
      361 iirherthvd rresvchlcq krfsrpdnlk lhmmihsgdk pyecgqcskr frqrvharrh
      421 eqvihsrryp ltcprcgaga edvtklrrht ckplgttegk egfkrgrgrp rlmadgtapk
      481 vrprntvegt qlqvgvkrgr grprkvkaqd asapap
//
LOCUS       XP_077517331             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128116 [Amblyomma americanum].
ACCESSION   XP_077517331
VERSION     XP_077517331.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661205.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144128116"
                     /calculated_mol_wt=14525
     CDS             1..133
                     /gene="LOC144128116"
                     /coded_by="XM_077661205.1:82..483"
                     /db_xref="GeneID:144128116"
ORIGIN      
        1 matngvasrq deakavepne etqsgrppap eyilleqwea hgsggpyfpe nsasceeqrd
       61 ekpfdgreiv aradaalddv mreaieafge tlrkslpwss pdalpdhwea pgsgdpnlpe
      121 epssqevqhd enr
//
LOCUS       XP_077517342             318 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128126 [Amblyomma americanum].
ACCESSION   XP_077517342
VERSION     XP_077517342.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661216.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..318
                     /product="uncharacterized protein LOC144128126"
                     /calculated_mol_wt=34622
     Region          <17..>152
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     CDS             1..318
                     /gene="LOC144128126"
                     /coded_by="XM_077661216.1:1..957"
                     /db_xref="GeneID:144128126"
ORIGIN      
        1 mtlgppptda paphqgslkm kdapsspeth tttqpptetr spttessggh ttrttsktsl
       61 gnmttekptp trvpeqttap drspganeta sdkqgmkdpp vpttnvsdsp ssndkdgtvc
      121 ptvhksfcnv ngaaqffydp degsclsatv qrahvcnrsp nqfasiedcl aacvkgrwap
      181 ehrcvqkprf vactaedarg dwwfhdgrkc vpwsfaggrc plasapvfdt aavcgaqcnd
      241 srtrrergcl lpvsqlceps qlrlpffaap svtdkgtafq chrvewefll rhrcllgsna
      301 fvnrdechet crrrtrqy
//
LOCUS       XP_077517349             318 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128133 [Amblyomma americanum].
ACCESSION   XP_077517349
VERSION     XP_077517349.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661223.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..318
                     /product="uncharacterized protein LOC144128133"
                     /calculated_mol_wt=34622
     Region          <17..>152
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     CDS             1..318
                     /gene="LOC144128133"
                     /coded_by="XM_077661223.1:1..957"
                     /db_xref="GeneID:144128133"
ORIGIN      
        1 mtlgppptda paphqgslkm kdapsspeth tttqpptetr spttessggh ttrttsktsl
       61 gnmttekptp trvpeqttap drspganeta sdkqgmkdpp vpttnvsdsp ssndkdgtvc
      121 ptvhksfcnv ngaaqffydp degsclsatv qrahvcnrsp nqfasiedcl aacvkgrwap
      181 ehrcvqkprf vactaedarg dwwfhdgrkc vpwsfaggrc plasapvfdt aavcgaqcnd
      241 srtrrergcl lpvsqlceps qlrlpffaap svtdkgtafq chrvewefll rhrcllgsna
      301 fvnrdechet crrrtrqy
//
LOCUS       XP_077517359             442 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128137 [Amblyomma americanum].
ACCESSION   XP_077517359
VERSION     XP_077517359.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661233.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 32% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..442
                     /product="uncharacterized protein LOC144128137"
                     /calculated_mol_wt=49351
     CDS             1..442
                     /gene="LOC144128137"
                     /coded_by="XM_077661233.1:1..1329"
                     /db_xref="GeneID:144128137"
ORIGIN      
        1 mggrlkrwlr davwpdllgy npticggvle dfsaaeyhgg tlrteelapr pqtssyggct
       61 rddvnradda nrasteafet pllddiycrr sdatedgtde tevvegnwad ldesrpqrle
      121 wqpshhhqrd errrsgladw eqvpigrgsr clfirpaptk rlvrrgsccr pvlriagraa
      181 sgtpsfagrv raaaeglqaa ggcrllrdpa gtelqaqdfg hrytvrgyds fvdcrrltfl
      241 dplpkarvcc lchdvlpvqy lapcgnavca scldvsikea pakcrerreg tspeghakfs
      301 mrgvtrldfn akdlgtlrvy cpniwygctq sselrhleeh ylkncphhpk mcfhcrredi
      361 ppgdfvphiy sckrrqeear svdrqsppvq qslsespasq tedehlcatq allaevterg
      421 gevhpepveh lnqlaadikr ap
//
LOCUS       XP_077517372             380 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128142 [Amblyomma americanum].
ACCESSION   XP_077517372
VERSION     XP_077517372.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661246.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..380
                     /product="uncharacterized protein LOC144128142"
                     /calculated_mol_wt=41471
     Region          <78..>213
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     CDS             1..380
                     /gene="LOC144128142"
                     /coded_by="XM_077661246.1:1..1143"
                     /db_xref="GeneID:144128142"
ORIGIN      
        1 mdavprvivr mltslamllt savivvgihm yartadkted tlsvggnqvn klrkpdprif
       61 dltlgppptd apaphqgslk mkdapsspet htttqpptet rspttessgg httrttskts
      121 lgnmtpekpt ptrvpeqtta pdrspganet asdkqgmkdp lvpttnvsds pssndkdgtv
      181 cptvhksfcn vngaaqffyd pdegsclsat vqrahvcnrs pnqfasiedc laacvkgrwa
      241 pehrcvqkpr fvactaedar gdwwfhdgrk cvpwsfaggr cplasapvfd taavcgaqcn
      301 dsrtrrergc llpvsqlcep sqlrlpfffa apsvtdkgta fqchrvewef llrhrcllgs
      361 nafvnrdech etcrrrtrqy
//
LOCUS       XP_077517382             309 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128152 [Amblyomma americanum].
ACCESSION   XP_077517382
VERSION     XP_077517382.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661256.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 32% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..309
                     /product="uncharacterized protein LOC144128152"
                     /calculated_mol_wt=34307
     CDS             1..309
                     /gene="LOC144128152"
                     /coded_by="XM_077661256.1:1..930"
                     /db_xref="GeneID:144128152"
ORIGIN      
        1 mvsvssdtdq plnvggrlkr wlrdavwpdl lgynpticgg vlevfsaaey hggtlrteel
       61 aprpqtssyg gctrddvnra ddanrastea fetpllddiy crrsdasedg tdetevvegn
      121 wadldesrpq rlewqpshhh qrderrrsgl adwelshvnn sglsedtayl alpenkcpse
      181 evlgacsfdq hqqnawcaaa aavapcfvsp veplraprhs qdvfeqllka crqpeavafs
      241 eilqelnapk lrtprsfvdr wrrhasqepa caslfqssct tartsagqrr fpaawrsmge
      301 qsssswdvc
//
LOCUS       XP_077517392             403 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128160 [Amblyomma americanum].
ACCESSION   XP_077517392
VERSION     XP_077517392.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661266.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..403
                     /product="uncharacterized protein LOC144128160"
                     /calculated_mol_wt=44131
     Region          <102..>237
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     CDS             1..403
                     /gene="LOC144128160"
                     /coded_by="XM_077661266.1:1..1212"
                     /db_xref="GeneID:144128160"
ORIGIN      
        1 mdavprvivr mltslamllt savivvgihm yartadmted tlsvggnqvn klrkpdprif
       61 dltlgppptd apaphqitrl hyskvldrys firnavvvei gslkmkdaps spethtttqp
      121 ptetrsptte ssgghttrtt sktslgnmtt ekptptrvpe qttapdrspg anetasdkqg
      181 mkdppvpttn vsdspssndk dgtvcptvhk sfcnvngaaq ffydpdegsc lsatvqrahv
      241 cnrspnqfas iegclaacvk grwapehrcv qkprfvacta edargdwwfh dgrkcvpwsf
      301 aggrcplasa pvfdtaavcg aqcndsrtrr ergcllpvsq lcepsqlrlp ffaapsvtdk
      361 gtafqchrve wefllrhrcl lgsnafvnrd echetcrrrt rqy
//
LOCUS       XP_077517400             124 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128168 [Amblyomma americanum].
ACCESSION   XP_077517400
VERSION     XP_077517400.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661274.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 35% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..124
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..124
                     /product="uncharacterized protein LOC144128168"
                     /calculated_mol_wt=13542
     CDS             1..124
                     /gene="LOC144128168"
                     /coded_by="XM_077661274.1:43..417"
                     /db_xref="GeneID:144128168"
ORIGIN      
        1 meatnpdvvp aspsssprae hnvgvedcet giavqtdlkm gdiealenec valnerlyas
       61 rtenkllelt edalkcsdak vvfytaglaa ifthmtqhsp qsirgrvtgq ywepvdiivh
      121 knmr
//
LOCUS       XP_077517454             112 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128184 [Amblyomma americanum].
ACCESSION   XP_077517454
VERSION     XP_077517454.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661328.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 31% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..112
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..112
                     /product="uncharacterized protein LOC144128184"
                     /calculated_mol_wt=11834
     CDS             1..112
                     /gene="LOC144128184"
                     /coded_by="XM_077661328.1:40..378"
                     /db_xref="GeneID:144128184"
ORIGIN      
        1 mstfhremqt pqddtaasdv asedmdisde pvtvgvlsdl sasqdvlpgs srqhdeimda
       61 ssppslqasn ilerpstsqc emaimwelqs eggtddgrgg ppilrsvpse dt
//
LOCUS       XP_077517561             112 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128211 [Amblyomma americanum].
ACCESSION   XP_077517561
VERSION     XP_077517561.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661435.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 44% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..112
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..112
                     /product="uncharacterized protein LOC144128211"
                     /calculated_mol_wt=12662
     Region          22..93
                     /region_name="GT1"
                     /note="myb-like, SANT family; cl23759"
                     /db_xref="CDD:474047"
     CDS             1..112
                     /gene="LOC144128211"
                     /coded_by="XM_077661435.1:1..339"
                     /db_xref="GeneID:144128211"
ORIGIN      
        1 maasmaamaa dpscvifsve mllnaikahp ilydkrhlnf kdtvicqkkf knlkdtfmki
       61 rnqqresmks gagladvpta kwrhfeqmqa fmetvsrepm lsssmdftlt qe
//
LOCUS       XP_077517587             660 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylinositol-3,5-bisphosphate 3-phosphatase MTMR14-like
            [Amblyomma americanum].
ACCESSION   XP_077517587
VERSION     XP_077517587.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661461.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..660
                     /product="phosphatidylinositol-3,5-bisphosphate
                     3-phosphatase MTMR14-like"
                     /calculated_mol_wt=73862
     Region          114..264
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     CDS             1..660
                     /gene="LOC144128227"
                     /coded_by="XM_077661461.1:87..2069"
                     /db_xref="GeneID:144128227"
ORIGIN      
        1 memaelvqpn dvtelvaifa nsvfdnttgd pektrmyaei qqkcvdlfak dyvvkvidne
       61 kgslsptypn rivslafpvr spegstayra ahalrtrklr ghmararsar ahkrfavpvi
      121 lydgkhicrs atlssgleiy wrtvleflgi sdrergeese sasekeaelc qysgavssyd
      181 vaigearpvl depstprkes vspltkadss rhselrmedi lllrelsvgq icdfmvegkk
      241 ekysvkvsss ekvdkwnryr dftifklpyp gceffeefka kgynaeglvf gwnqnfvnve
      301 levpkdqfas elgidwaayk ewdlvqltkn ylkyllkvvf dgssgllvhc icgwdrtplf
      361 iamlrlslwa dgaihqnlsa veiayltvay dwflfchdlp nrvnkseeif ffcfnmlkyi
      421 dtedfsvltl ktrkddlems dsniqsthlg eqeldvgtsy taresgsasg wdlgepsvyf
      481 qvpsdsteqr sfdhgagvri ggedassaks sdeddsavvv pstgaaaspw dtlsvrqrht
      541 sgcssfgdgs wqvvnetgsv ggsppsllsr rkygrsqsps prsrpdilek ptdtssklls
      601 saellqrrre rleavrrmvs rpyqsivadk tnrragrrps swtrrllsia ralfpgggcf
//
LOCUS       XP_077517635             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128247 [Amblyomma americanum].
ACCESSION   XP_077517635
VERSION     XP_077517635.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661509.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 12% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144128247"
                     /calculated_mol_wt=17715
     CDS             1..158
                     /gene="LOC144128247"
                     /coded_by="XM_077661509.1:1..477"
                     /db_xref="GeneID:144128247"
ORIGIN      
        1 mscafqdlvl llqenvasvv lvfkvlprhf deprkaqfed rrrrqlnrrl satlkrlsgv
       61 hilnpehrfl dasgkpmlsl faadrynvar drgidqmski ivaalvkiyg pgiasasrar
      121 pgevyvvhrc rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077517656             601 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: kinesin-like protein KIF20A [Amblyomma
            americanum].
ACCESSION   XP_077517656
VERSION     XP_077517656.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661530.1
KEYWORDS    RefSeq; corrected model; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio   :: 1% of CDS bases
            frameshifts :: corrected 2 indels
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..601
                     /product="LOW QUALITY PROTEIN: kinesin-like protein
                     KIF20A"
                     /calculated_mol_wt=66270
     Region          45..446
                     /region_name="Motor_domain"
                     /note="Myosin and Kinesin motor domain; cl22853"
                     /db_xref="CDD:473979"
     Site            order(51..52,126..133,312..313,350..355)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:276819"
     CDS             1..601
                     /gene="LOC144128261"
                     /coded_by="XM_077661530.1:1..1806"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 3 bases in 2 codons"
                     /db_xref="GeneID:144128261"
ORIGIN      
        1 mhrparpyed stesaqlpem epelspvesv rqkldksiva ssaskvylrl rprvggkpft
       61 npafephset tvasttatle hqqqkcfnfn mvfpegssqe qlfqgvvqds ldafiegrnv
      121 llfahgptas gkthtmqgpc tdpgivlrtl drlfrllgtq icqgaavrpd cfdevvqlst
      181 teeaavlqlk nqllgekgar saadcstyhl phngsdaslf lslsssgsnd sagqvllwls
      241 fyeinneriy dlllpsaeap tkkakgtcrt llrlgedhak rffirgliev pihtadeahr
      301 llclayhnrs iaethlnrns scshsvftvr lvasgagsqd whvsslmlcd lagsekpsks
      361 gtngsqlrea grintslmvl srcleglcrn kdaakkvpvp frdskltkvm qayftmggqi
      421 slvvnicpsm amfqeslnal kfsgiacqvv plqleprher crkavrrlte vwsnarastg
      481 egdsfmaads sagpaildra etdelfdtvt alqqeldntq kalrwnerml sasraevtdy
      541 knlvaemekt hellriadke matrirnace itrldlyrta eagstmehlg rleeaerrva
      601 e
//
LOCUS       XP_077517670             189 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128267 [Amblyomma americanum].
ACCESSION   XP_077517670
VERSION     XP_077517670.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661544.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 31% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..189
                     /product="uncharacterized protein LOC144128267"
                     /calculated_mol_wt=20397
     CDS             1..189
                     /gene="LOC144128267"
                     /coded_by="XM_077661544.1:1..570"
                     /db_xref="GeneID:144128267"
ORIGIN      
        1 maaeercskl ameledttes aceakqsnan lpdcleelaa slppggdepa adenhprttw
       61 rttrasratr ttsatrkeat dgesdcrtra qrprrvkksv avsedplddd ddiacrgsvl
      121 vdgnsefspl kcpgemvvgm fsrssndgat astcktrrll ppdqdfveve deepppsalk
      181 ttgcrrakh
//
LOCUS       XP_077517695             189 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128277 [Amblyomma americanum].
ACCESSION   XP_077517695
VERSION     XP_077517695.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661569.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..189
                     /product="uncharacterized protein LOC144128277"
                     /calculated_mol_wt=20408
     CDS             1..189
                     /gene="LOC144128277"
                     /coded_by="XM_077661569.1:38..607"
                     /db_xref="GeneID:144128277"
ORIGIN      
        1 mralgisiks arlkpgvvps vfphnrstsp ppraafakrk rrevlvqlle niapqevaas
       61 lspspsealv aesvaeaddv annvpdttdk sfqvvvrppt lckglqanvk apktsrktqt
      121 qpqgvsvgvq vgsflvdaat qvnfmvepye eddiltdcep epeyhddysp dsdswhecdn
      181 cdghlcsqq
//
LOCUS       XP_077517763             351 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128315 [Amblyomma americanum].
ACCESSION   XP_077517763
VERSION     XP_077517763.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661637.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..351
                     /product="uncharacterized protein LOC144128315"
                     /calculated_mol_wt=39689
     Region          214..302
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(214..218,222..223,256,258..259,298..299)
                     /site_type="active"
                     /db_xref="CDD:238180"
     Site            order(214..218,222,258)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238180"
     Site            223
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238180"
     CDS             1..351
                     /gene="LOC144128315"
                     /coded_by="XM_077661637.1:1..1056"
                     /db_xref="GeneID:144128315"
ORIGIN      
        1 msssivvlpa dkgnstvvln qsdyirkmtt llqdeamykk vkrdptkkve telqsllsdv
       61 fkfippekkh lyfrllchng sapaiyglpk vhkpdvplrp ivdftrsplh alsgylhqvi
      121 rpvvgrrtty vkdsshfvnk lkdvtidded vlvsfdvksm ftsvpvdfav ssckrllend
      181 aslpsrtpie vvdlcrlldf clrntyfvfr kqhykqlfgt amgasvsvvc anivleriea
      241 ealgsfhpap klflryvddc fcvirrqdas hflehlnsfq ptiqftkekq qneaipfldv
      301 lvqrtsngla tcvyrkptht grylsfhsah pvahkrsvas salfgrafrl c
//
LOCUS       XP_077517798             153 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128337 [Amblyomma americanum].
ACCESSION   XP_077517798
VERSION     XP_077517798.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661672.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..153
                     /product="uncharacterized protein LOC144128337"
                     /calculated_mol_wt=17247
     CDS             1..153
                     /gene="LOC144128337"
                     /coded_by="XM_077661672.1:44..505"
                     /db_xref="GeneID:144128337"
ORIGIN      
        1 meaesaaava taalvilell eeppakkprt swvspwlqrr kqqgayenlm relaledsen
       61 yrrwirmdta tfeellgevr phitkkdtpf reaiqagerl aitlrylatg nsemslqfaf
      121 yvahntisgi isevsqaiyr vpkpkvlkdg nqc
//
LOCUS       XP_077517836             154 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128359 [Amblyomma americanum].
ACCESSION   XP_077517836
VERSION     XP_077517836.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661710.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 34% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..154
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..154
                     /product="uncharacterized protein LOC144128359"
                     /calculated_mol_wt=16890
     Region          116..>146
                     /region_name="FH_FOX"
                     /note="Forkhead (FH) domain found in Forkhead box (FOX)
                     family of transcription factors and similar proteins;
                     cl00061"
                     /db_xref="CDD:469596"
     CDS             1..154
                     /gene="LOC144128359"
                     /coded_by="XM_077661710.1:1..465"
                     /db_xref="GeneID:144128359"
ORIGIN      
        1 mhhesgaadv eplcveveee tdalsnayvs simpdmstiv qaasdvpsca grqhlcdeps
       61 tkrrrgaglq stfrrqnqpn tedtctvdrv ppgplgellp agtclrrpsg flvehianai
      121 hksprqmlra shiyealrnk fpfyrfvdmd aqat
//
LOCUS       XP_077517849             361 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128362 [Amblyomma americanum].
ACCESSION   XP_077517849
VERSION     XP_077517849.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661723.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 24% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..361
                     /product="uncharacterized protein LOC144128362"
                     /calculated_mol_wt=40238
     CDS             1..361
                     /gene="LOC144128362"
                     /coded_by="XM_077661723.1:1..1086"
                     /db_xref="GeneID:144128362"
ORIGIN      
        1 mpekeeqgqp lettealtsa gdlglmpssa rtppnleppr dikmssvptt gasqlpmvss
       61 qsqeesstsn splpepaprp ssapprlgra tsqqtalplv vgrsreqhqp qagpqpqpql
      121 qpqrqfvgwp hgnaerfyal hvpslqdyap qpqpqlqpqr qfvgwphgna esfyalhvps
      181 lqdyaqqemq ppygrrrpgd nyslswdpha rvgnvlsedn fysgrpygtq spprpqttih
      241 sarhgpelqw tptdasfvql lpqresplsr qeppsrrerp aspqglssqq drqsllhedl
      301 lseheqpllr syrpqqqgmp rveldairln grpyvvrnar rsaddpravi iecyaalerp
      361 t
//
LOCUS       XP_077517861             829 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128370 [Amblyomma americanum].
ACCESSION   XP_077517861
VERSION     XP_077517861.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661735.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 36% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..829
                     /product="uncharacterized protein LOC144128370"
                     /calculated_mol_wt=91095
     Region          <22..294
                     /region_name="COG5025"
                     /note="Transcription factor of the Forkhead/HNF3 family
                     [Transcription]"
                     /db_xref="CDD:227358"
     Region          117..183
                     /region_name="FH_FOX"
                     /note="Forkhead (FH) domain found in Forkhead box (FOX)
                     family of transcription factors and similar proteins;
                     cl00061"
                     /db_xref="CDD:469596"
     Site            order(131..132,135,158..159,161..162,171,178..180,182)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410651"
     Region          <249..>343
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          434..508
                     /region_name="Forkhead"
                     /note="Forkhead domain; pfam00250"
                     /db_xref="CDD:459732"
     CDS             1..829
                     /gene="LOC144128370"
                     /coded_by="XM_077661735.1:1..2490"
                     /db_xref="GeneID:144128370"
ORIGIN      
        1 mhhesgtadv kplgaeveee tdalsnayvs simpdmstvv qaasdvpssa ghqhrcgkps
       61 tkkrhrgaaw qwtvrrqklp siedtctvdr tppgplgell pagtclrrpp gflvqhianv
      121 ihksprqmlr vqhiyealrn kfpffrfvdk dahatfkssv rhalfqrwfi kvpssnatns
      181 fwsvnyserp hdwimpkkee qgrpnettea lnfasdlgpv pspartppdl epprdirmss
      241 vptagasqlp mvssqsqekt stsssplpvl lsrttqatpk sspsdsnpls ptpqyrdtgv
      301 sssaparaep tpvgmctglk etptvdcpgp lpmqveeetd alsnayvssi mpdmstivqa
      361 asdvpscagr qhlcdepstk rrrgaglqst frrqnqpnte dtctvdrapp gllgellpag
      421 tclrrpsgfl vehianaihk sprqmlrash iyealrnkfp fyrfvdmdaq atwmssvrha
      481 lcqcwfikvp sskarnsfws lnyserprvw impekeeqgq pletteasts agdlglmpss
      541 artppnlepp rdirmssvpt tgasqlpmvs sqsqeessts nsplpvllpr stqatpkssp
      601 sscnpvsptp qyrdtavsns aparaepapv grcigvkdtp tvkypgpfpv qepaprpssa
      661 pprlgratsq qtalplvvgr sreqhqpqag pqpqpqlqpp rqfvgwphgn eesfyalsva
      721 slqdyaqqem qpshgrrckh pynppfcggm svelssqqdr qsllqeells eheqpvlrrc
      781 rpqqqgvprv eldvtrlngr pyvvqnarrs addpraviie cyaalerpt
//
LOCUS       XP_077517872             290 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128380 [Amblyomma americanum].
ACCESSION   XP_077517872
VERSION     XP_077517872.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661746.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 37% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..290
                     /product="uncharacterized protein LOC144128380"
                     /calculated_mol_wt=32122
     CDS             1..290
                     /gene="LOC144128380"
                     /coded_by="XM_077661746.1:1..873"
                     /db_xref="GeneID:144128380"
ORIGIN      
        1 maasgidfqs llwlsdmasq eqqvdaartl tgivqnnllt papvarprlr vdlpsyggyh
       61 dhvsaneyld rmlnyqqatg ltddevlarv vpvsltdqaa rwfrltghrl rtmeefrakf
      121 ceeflpadce rrlrrelelr iqppdeslle yvrtmdelyr iadppapnge kvervtrqah
      181 ptfaaylrgv qfrdvdelal aakriqadil ashayrpppp asqaleprci wnggtfrirs
      241 rgaetvafgd gqggtvnrre ettappgngr ipsatsatne gttpgnadap
//
LOCUS       XP_077517883             565 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128388 [Amblyomma americanum].
ACCESSION   XP_077517883
VERSION     XP_077517883.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661757.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 18% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..565
                     /product="uncharacterized protein LOC144128388"
                     /calculated_mol_wt=63036
     Region          210..290
                     /region_name="FH_FOX"
                     /note="Forkhead (FH) domain found in Forkhead box (FOX)
                     family of transcription factors and similar proteins;
                     cl00061"
                     /db_xref="CDD:469596"
     Site            order(214,232..233,236,259..260,262..263,266,272,286..288,
                     290)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410651"
     CDS             1..565
                     /gene="LOC144128388"
                     /coded_by="XM_077661757.1:132..1829"
                     /db_xref="GeneID:144128388"
ORIGIN      
        1 mddsdwtwdg dgfqptkrpk legssstrks krprrkrsgg drsrakprer skhplkknee
       61 cgakrgrrgr drvptkekkk lgssavaqpp pgttmecghv pnteeeppav vynedlledl
      121 areyletikp dlstvvrlkg rdssltshsr rsrslgyvdt tkrrrassep akrsgrtrtm
      181 sspagtctva klppgplael lpagtivrrp pgklvqlvaq aihesplqvl rvrhvyealk
      241 krypyfmfmd kkdettwkss vrhalsqrwf kkvhthppdr naargksnfw einyhnrprg
      301 wampeeeems stgfgespes tpsvwsqdld meldllpplq spppqqqact pyqvllspqt
      361 amqrqqppva rpmpthssap tlqsssaeps lmsplymlhm sppawctgmd eipvgselgp
      421 lcaqekhqeq eavmpqeqsl prlgpsnsqe gtlphpelpl ppqfswppqe pplpggptsp
      481 prqeetgpwg tgpgfrprsg rlqlpgwpyv gepvfaaian prravmmedg twyilvypaf
      541 aendeclara vwhweegtpa daval
//
LOCUS       XP_077517896             195 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128392 [Amblyomma americanum].
ACCESSION   XP_077517896
VERSION     XP_077517896.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661770.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 12% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..195
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..195
                     /product="uncharacterized protein LOC144128392"
                     /calculated_mol_wt=21729
     CDS             1..195
                     /gene="LOC144128392"
                     /coded_by="XM_077661770.1:1..588"
                     /db_xref="GeneID:144128392"
ORIGIN      
        1 mtgesvtsty vlrrsiarad lsrtdtprsv attpevadli aerpvtkplc aafltavgnp
       61 lsipalalmv ftrresvllr riqtgtilnp flvdrfhwdg rmppvsgsws rcncganmnl
      121 fwecplytpp rlralatfkq epwtssiwtw acpdtsspdc aielwrallv fihdseaslv
      181 derlrnaler htast
//
LOCUS       XP_077517918            1470 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128410 [Amblyomma americanum].
ACCESSION   XP_077517918
VERSION     XP_077517918.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661792.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1470
                     /product="uncharacterized protein LOC144128410"
                     /calculated_mol_wt=166128
     Region          506..1066
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1470
                     /gene="LOC144128410"
                     /coded_by="XM_077661792.1:1..4413"
                     /db_xref="GeneID:144128410"
ORIGIN      
        1 mapttafcar dredncrema lrvhcldsgw gtptasqlrr llglqcavgq vvagdcgeva
       61 vyesptwtan vtlqlerspk dsfmhivlkw cdqcqnasnn gaaagksgpp ewwlaseele
      121 efcdpystqi atehkdvpvn afalgcflgy ntfvahfdst ytmppppspl ssakssssvs
      181 gksmsvkrgk kgravksssp sdgtsssakt scpnseltss egfkvtqcna hfehcsngly
      241 lyfvdvrkrs stlfrllvry sticriilrk tsptstivyl qllhppllyk vcagnsatsq
      301 cnregdylpq fdlsdttpwd ravefgegpf kcgadvlgrs rmmrltlsgp rhwaplcnli
      361 lrtrknaqvf yapvrelhar elkavarpsv qdfgvvyall svwsesfqvt delamdaqsc
      421 retllelsar aeecpralee alfeiyfsls qgkvfwfrsa lehlyfryrv hrqridvftg
      481 sviaqelpsh mvkvrkvilt ptrrvymppq lvcksrllqh cdpdyalriv vrdddcklis
      541 fslgackddf lsavikppls sglkiggrrf mflgcstsql rshgvwfyah dnqgrtaeqi
      601 rdgigdlsti vsvpkfmarl gqafsqslgl ltvpsqntsv epdirhaapg rssdgshrey
      661 ifsdgigris hgllrkvhha leleegeepc avqiryggck gmllmdptls gcrivfresm
      721 rkfhsdhsdl yvlktskprv lylnrpmiti leqsgikaev flmlqnkild sfidsmmdph
      781 eaahvlhsyc alrlpykela gvgidltvep ffralvravn kkvlkelrtk arilvppnyg
      841 rtmfgvldet gtleygqvfv qysndmlryk vndpatileg dvivtknpcm npgdirklea
      901 vnvpqlhhvr dcivfpqkge rphpdemags dldgdeysvl wyedlifnnn cnpmhyhsdp
      961 pkerkasigv qdmvdffcqy ikgdkiglia nahlvwadil dsginshrcr elarkcavnl
     1021 dfakcgdlkg fqnsekppmy pdfmekldtk ntycsrkvlg qlyrnckkve lsteclevve
     1081 eslpdprlll egreqflkea tsaykryakk irallksyri eteseafsga vsklskymke
     1141 ndptdmamvl esqvehvvrr treeffseql deaheklkas awyqvtyelq sseggiqsfp
     1201 wvvsdvlmri vvntssclpv pasrnsfcqr lgalllglph pgggdqdgtq hgdtqvltnl
     1261 lrlmydwids sreflfvknt eelgvyksim reacfkvsrs isrdmpphkl vilclrfaca
     1321 wclkifqggs dgeviskecr rryrlghlal itlnrlsmsg nlaylrrape gcpsteliri
     1381 yinredeeff eilrryedii krimmdwsgv ediqcdlktd rmdewflqlm vtgsrwaler
     1441 lkeivvypsf revlllafer eknaiggtlq
//
LOCUS       XP_077517952             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128431 [Amblyomma americanum].
ACCESSION   XP_077517952
VERSION     XP_077517952.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661826.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 15% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144128431"
                     /calculated_mol_wt=24251
     Region          10..104
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     CDS             1..217
                     /gene="LOC144128431"
                     /coded_by="XM_077661826.1:1..654"
                     /db_xref="GeneID:144128431"
ORIGIN      
        1 msrglfnevl iievskrpll wdvkdnfrnk stkeslweev rdairaiddt vtveeiiarw
       61 knlkdtyrrk ikdekdgkks gsgataktaw phlkqmeflr dsmetrrsfc nldavshvqv
      121 asaaaetsha scspaspdsk eveatpnefe cmysvpglpd epgpscmaat spevlphrgn
      181 kpqkkrrqev eaadrdiqav rsaiaahrpm vtphvrt
//
LOCUS       XP_077518000             164 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128454 [Amblyomma americanum].
ACCESSION   XP_077518000
VERSION     XP_077518000.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661874.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 25% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..164
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..164
                     /product="uncharacterized protein LOC144128454"
                     /calculated_mol_wt=18865
     CDS             1..164
                     /gene="LOC144128454"
                     /coded_by="XM_077661874.1:1..495"
                     /db_xref="GeneID:144128454"
ORIGIN      
        1 msddpqrqel ilrcylsnlh teggywygyl kcendllrfe rslaarheky svrtsrefda
       61 apkarfvssm ihcghkigld lqfrvtketv kvcmfklnvk gtkkkgchat lslkrielfp
      121 dfewgygptf vrattttatl yilegqqeli qqvrraakas rilk
//
LOCUS       XP_077518014             386 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4B chain-like [Amblyomma americanum].
ACCESSION   XP_077518014
VERSION     XP_077518014.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661888.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..386
                     /product="tubulin beta-4B chain-like"
                     /calculated_mol_wt=42688
     Region          <46..386
                     /region_name="PTZ00010"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:240228"
     CDS             1..386
                     /gene="LOC144128462"
                     /coded_by="XM_077661888.1:1..1161"
                     /db_xref="GeneID:144128462"
ORIGIN      
        1 mtegrfekte qasdltwnea fsleepkkpg sagrghnacd eqgsmgqsga gnnwakghyt
       61 egaelvdsvl dvvrkeaesc dclqgfqlth slgggtgsgm gtlliskire eypdrvmnty
      121 svvpspkvsd tvvepynatl svhklventd etycidneal ydicfrtlkl ttptygdlnh
      181 lvsatmsgvt tclrfpgqln gdlrklvvnm vpfprlhffm tgfaplvprl pavpgsdcag
      241 adatdvrcqn lmaacdprhg ryltvaavfr grmsmrevdd qmlniqnkhs syfvewipnk
      301 vktavcdipp rglkmsatfi gnstavrelf rriseqftam frrkaflhwy tvegmdelef
      361 teaesnlndl vseyqqyhea taedgg
//
LOCUS       XP_077518023             213 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128469 [Amblyomma americanum].
ACCESSION   XP_077518023
VERSION     XP_077518023.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661897.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..213
                     /product="uncharacterized protein LOC144128469"
                     /calculated_mol_wt=23758
     Region          67..88
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          95..115
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(100,102,104,106..107,110..111,114,128,130,134..135,
                     138..139,142,156,158,160,162..163,166..167,170)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          107..132
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          123..143
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          147..>209
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          151..171
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          179..200
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..213
                     /gene="LOC144128469"
                     /coded_by="XM_077661897.1:451..1092"
                     /db_xref="GeneID:144128469"
ORIGIN      
        1 mwlrchwvsd prayvppcpp yhqphhevkt ppveprfvgt lqlvpqavpa aaaagppgld
       61 agtgfwcdlc gkvsdrrqal lshgcrshgi crfvcthcga afrqsghltn htrthtgerp
      121 fpcqvcqrsf aqksnlvdhm rthtgerpyr cdicqstftr kahlvghtrm htgerpfqcp
      181 acpkafatss alnqhrhfth rnpendnspr pll
//
LOCUS       XP_077518033             448 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128477 [Amblyomma americanum].
ACCESSION   XP_077518033
VERSION     XP_077518033.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661907.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..448
                     /product="uncharacterized protein LOC144128477"
                     /calculated_mol_wt=50720
     Region          23..44
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          67..83
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          213..307
                     /region_name="MULE"
                     /note="MULE transposase domain; pfam10551"
                     /db_xref="CDD:463145"
     CDS             1..448
                     /gene="LOC144128477"
                     /coded_by="XM_077661907.1:124..1470"
                     /db_xref="GeneID:144128477"
ORIGIN      
        1 mqtdasagaa sattgknser kwckfcdkaf sntsnlrkhv knvhgegalq hvkdeealtm
       61 kvrsfscdlc pkhfvilrdl rrhhvdehnf eeevenlvfd sntgrlaegv paktvvhnir
      121 agtsgssprm qlvttkdlrn iadkfrigke eqrdpddiks vdlwveemra enespllyym
      181 kkgdpnshdg dlelalmtfp qrellsklgt eklcidsthg tnkykfqltt lltisedgsg
      241 ipcayviskr vnmgtmvrff eaikektntv qcqafmsdda pvygnaweqv mgtlksrllc
      301 swhvlhnwga rlntvtnkdc rdtirdmlht lmkctdetef qqllpaflsi yskeqqlnrl
      361 apkeleeldg firycnvkmc tpkgkniftg kqltatqpnn rareaqqqkr pssssrskre
      421 rtkherskhk hnrsitpggk stvpvlsg
//
LOCUS       XP_077518058             437 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128493 [Amblyomma americanum].
ACCESSION   XP_077518058
VERSION     XP_077518058.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661932.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..437
                     /product="uncharacterized protein LOC144128493"
                     /calculated_mol_wt=50083
     Region          <194..>324
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Region          263..358
                     /region_name="MULE"
                     /note="MULE transposase domain; pfam10551"
                     /db_xref="CDD:463145"
     CDS             1..437
                     /gene="LOC144128493"
                     /coded_by="XM_077661932.1:16..1329"
                     /db_xref="GeneID:144128493"
ORIGIN      
        1 mkvrsfscel cpkhfvilrd lrrhhvdehn feeevenlvf dsntefctwk dgeesrtcch
       61 fiastgrkes assvfkrylh chrsgthkel pieqrqrqkk gqgscrigkh cfatlevtek
      121 dskvyvvfqk khyghfnevc hqrlsqdear vlagrlaegv paktvvhnir agtsgssprm
      181 qlvttkdlrn iadkfrigke eqrnpddfks vdlwveemra enespllyym kkgdpnshdg
      241 dlelalmtfp qrellsklgt eklcidsthg tnkykfqltt lltisedgsg ipcayviskr
      301 vnmgtmvrff eaikkkqtnt vqcqafmsdd apvygnaweq vmgtlksrll cswhvlhnwg
      361 arlntvtnkd crdtirdmlh tlmkctdete fqqllpafls iyskeqqlnr lapkeleeld
      421 gfisvtalnt fqgkcsk
//
LOCUS       XP_077518080             414 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128510 [Amblyomma americanum].
ACCESSION   XP_077518080
VERSION     XP_077518080.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661954.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 16% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..414
                     /product="uncharacterized protein LOC144128510"
                     /calculated_mol_wt=44271
     CDS             1..414
                     /gene="LOC144128510"
                     /coded_by="XM_077661954.1:39..1283"
                     /db_xref="GeneID:144128510"
ORIGIN      
        1 mhkteclndt ssqlgactst dcglstcflp aspildappl ldavvdsvwq rgtltadrav
       61 qkcapfdapl ghgtataipr splmylqvll liflgrpwtm hkteclndts sqlgactstd
      121 cglstcflpa spildappll davvdsvwqr gtltadravq kcapfdaplg hgtataiprs
      181 plmylqvlll iflgrpwtmh kteclndtss qlgactstdc glstcflpas pildapplld
      241 avvdsvwqrg tltadravqk capfdaplgh gtataiprsp lmylqvgyyd aysyrdddvc
      301 llavswpphv lksfragvsy lwcllvlggd velnpgpmtk aqeekldlva ssilrleasn
      361 avlqdsvnkv lqihadlkid lekltkrvhd leqkfataps aelvgnsdkg lana
//
LOCUS       XP_077518091             360 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128519 [Amblyomma americanum].
ACCESSION   XP_077518091
VERSION     XP_077518091.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661965.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..360
                     /product="uncharacterized protein LOC144128519"
                     /calculated_mol_wt=40343
     Region          5..95
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          277..>341
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..360
                     /gene="LOC144128519"
                     /coded_by="XM_077661965.1:1..1083"
                     /db_xref="GeneID:144128519"
ORIGIN      
        1 mpgcccapgc rsnydggpsv rvykfpadet qraawtkais redfvptkht vvcekhfqts
       61 dfvrfatytd entgnvievp lqrvrlkpaa vpsvfpgcpk ylsqppssre apaekkarle
      121 aaslrkaiel svaaqeeedr rnkicsfgel csalsklkts dfwtvvttqa kvlflelsld
      181 qapavrssvv vlddlsvqvf lggsrlhnlg decvpsqikd lreldkvlqs veklqscasg
      241 sekehqllat vlmlldevnk dyelqehhgw nlqvlkfvrs qvelvlknvp ryppdvlvfs
      301 gllytisphv yrfirsslki klphpdtirr lcssyhmnpa leqqdpcfln yaktvlprel
//
LOCUS       XP_077518103             406 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128528 [Amblyomma americanum].
ACCESSION   XP_077518103
VERSION     XP_077518103.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661977.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 50% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..406
                     /product="uncharacterized protein LOC144128528"
                     /calculated_mol_wt=44997
     Region          54..136
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     Region          275..>302
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            289..291
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133137"
     Site            289
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133137"
     CDS             1..406
                     /gene="LOC144128528"
                     /coded_by="XM_077661977.1:43..1263"
                     /db_xref="GeneID:144128528"
ORIGIN      
        1 msrlgridey dpkvqnfdsy lerfehfvsa nevseakels vfltvlgaea yevlknlvvp
       61 alpgektfae ikillknhys pqtsviaerc rfnrrvqleq esvedfvlel khlarkcnfg
      121 dflqdalrdr lvagirneet qralfttegl tfqgackial drelatqqaa llqqrgrsea
      181 lnavgtherc drktkdstrg krqkqkcsrc gkahtsdscw ykrykcrrcs kvghlqnmcq
      241 asreelkahl vsesseeest lyscdatpsk ksyvvsvnve ghdlpmqvdt gaavtvvpes
      301 vyaaklshvk sslsqqaslp seqprespgs agptvettcp eetssagtna pasppspaad
      361 stvpldasgd sresslpand davvpprrsq rmrrppdwfr psdfkk
//
LOCUS       XP_077518116             151 aa            linear   INV 25-NOV-2025
DEFINITION  palmitoyltransferase ZDHHC15A-like [Amblyomma americanum].
ACCESSION   XP_077518116
VERSION     XP_077518116.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077661990.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..151
                     /product="palmitoyltransferase ZDHHC15A-like"
                     /calculated_mol_wt=16880
     Region          1..>151
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; cl19890"
                     /db_xref="CDD:418707"
     CDS             1..151
                     /gene="LOC144128533"
                     /coded_by="XM_077661990.1:1..456"
                     /db_xref="GeneID:144128533"
ORIGIN      
        1 matssggses arrrrsnhef vawlpvamvv glfvwayyvy ifvfsgslvk egalrfafst
       61 vfhlllllcl wsfvqttvtt vppipgyfgl sesdqrlleq caddeargef ldileenrga
      121 ltrgpsggvr fcercqqvkp drahhcsqcr r
//
LOCUS       XP_077518190             411 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128566 [Amblyomma americanum].
ACCESSION   XP_077518190
VERSION     XP_077518190.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662064.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..411
                     /product="uncharacterized protein LOC144128566"
                     /calculated_mol_wt=46069
     CDS             1..411
                     /gene="LOC144128566"
                     /coded_by="XM_077662064.1:326..1561"
                     /db_xref="GeneID:144128566"
ORIGIN      
        1 mlnlqlcaav lfsgcnptat lrmlasigap vvsnrtffai qrsylwpaid rvwkeeqksl
       61 lqelqgqqvn lagdgradsp gfsakygtyt lmdverhkvl hfevvqsnda ggccrmeleg
      121 lkqglafles esikvavlvt drhtqikcfl rnnkaaiahe fdvwhmakgi tkklhtaakq
      181 nnckelapwi ksvcnhlywa aassegkael ivpkwlslln hirdihthdq asfptclhgd
      241 igpkqwlree skafqklqdi atskallqdm prlstryqty gleafhslla hfapktyhys
      301 ylgmrartql avlhynensg rgqartkdgt lrwcvrypka aggdptacpv kespthgyvk
      361 rlldkvvama esmqpqerkt apesrpplss sfpkapkekl vekrksgfnr q
//
LOCUS       XP_077518203             243 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128574 [Amblyomma americanum].
ACCESSION   XP_077518203
VERSION     XP_077518203.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662077.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..243
                     /product="uncharacterized protein LOC144128574"
                     /calculated_mol_wt=25518
     CDS             1..243
                     /gene="LOC144128574"
                     /coded_by="XM_077662077.1:1..732"
                     /db_xref="GeneID:144128574"
ORIGIN      
        1 mtrakgtggg rlcglrgril alvgrssavg vcegffeeds slppsaqsld ghasslqltp
       61 ttavqpitap adpatprlrr fpsrvsvgdr pgtsglsrts qqqerctpsr rpaaedflpe
      121 ivagyqksld laaeikselq qlkdevgava tsfqreaetm lgqsatlnrl aaatealads
      181 qarqataler maaiqengla nesanqariv aaverlaitg qfilqqvqwl stvtpisgep
      241 pak
//
LOCUS       XP_077518227             300 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128585 [Amblyomma americanum].
ACCESSION   XP_077518227
VERSION     XP_077518227.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662101.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 24% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..300
                     /product="uncharacterized protein LOC144128585"
                     /calculated_mol_wt=33236
     Region          212..282
                     /region_name="BEN"
                     /note="The BEN domain is found in diverse animal proteins;
                     smart01025"
                     /db_xref="CDD:214981"
     CDS             1..300
                     /gene="LOC144128585"
                     /coded_by="XM_077662101.1:1..903"
                     /db_xref="GeneID:144128585"
ORIGIN      
        1 mtyvlvqwvk tgsaavfdvi svrdivetkf tsailrnakd tsvwnthvhv rwrgekhpak
       61 iiavgtqtqm ekmcrqeikr pettcdgaae psnvicetct slyiengdlk eqlallqeen
      121 irlkatidlm teqkggieef teamrdsrll hgqlqaagtl rvnhysgakf qemleftsvv
      181 hvddledigg clvaksvskm ldvgprktas tyarellrqv fdpaefvnks itgrqsnahk
      241 gkdakpqldp frmnavikyt ckkfglirea pirqslssml nkhkaevedl nggaagdggq
//
LOCUS       XP_077518243             335 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128588 [Amblyomma americanum].
ACCESSION   XP_077518243
VERSION     XP_077518243.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662117.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 61% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..335
                     /product="uncharacterized protein LOC144128588"
                     /calculated_mol_wt=37695
     Region          <2..275
                     /region_name="transpos_IS630"
                     /note="IS630 family transposase; NF033545"
                     /db_xref="CDD:468076"
     CDS             1..335
                     /gene="LOC144128588"
                     /coded_by="XM_077662117.1:32..1039"
                     /db_xref="GeneID:144128588"
ORIGIN      
        1 mskatlwrll kemgfkfekr krtlaliers divawrrryl raikafrsqg rcivyldetw
       61 vnaghtrqhv wkdstvkssq daflrglttg lpapsgkggr lilvhagssl tgfvsgaadf
      121 frakkgasad hhsemdgkyf eewfsskllp nipnssiivm dnapyhsval ekppttstrk
      181 ieiqawltrk giswstdmvr aelldlckrt tsghtvyaid tlaakhghev lrlppyhcef
      241 npieqvwsmv kgfvakrnka ftlaeveell pqalahvske dwqkfcahae riedeawerd
      301 iifddevdpv vfaigsssss seessselsg ieele
//
LOCUS       XP_077518254             199 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128595 [Amblyomma americanum].
ACCESSION   XP_077518254
VERSION     XP_077518254.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662128.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..199
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..199
                     /product="uncharacterized protein LOC144128595"
                     /calculated_mol_wt=22254
     Region          15..174
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..199
                     /gene="LOC144128595"
                     /coded_by="XM_077662128.1:1..600"
                     /db_xref="GeneID:144128595"
ORIGIN      
        1 mpaairtypt trvildatei kcevpfslsl qsssystykt sntfkghigv spnclvsfvs
       61 elytgsisdr eaivksdfln lkfdkndsvm adkglliedl lasknvkldi ppflhrnals
      121 eqqvretkei aslrihverr irrvksfhif drpipltlap iinqiwtvaa iltnfqsplg
      181 kpsqpsrqql atptelmne
//
LOCUS       XP_077518266             747 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128605 [Amblyomma americanum].
ACCESSION   XP_077518266
VERSION     XP_077518266.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662140.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 22% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..747
                     /product="uncharacterized protein LOC144128605"
                     /calculated_mol_wt=83465
     Region          488..>658
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     Region          656..>745
                     /region_name="DR0291"
                     /note="Predicted nucleic acid-binding protein DR0291,
                     contains C4-type Zn-ribbon domain [General function
                     prediction only]; COG1579"
                     /db_xref="CDD:441187"
     CDS             1..747
                     /gene="LOC144128605"
                     /coded_by="XM_077662140.1:1..2244"
                     /db_xref="GeneID:144128605"
ORIGIN      
        1 malrvhclds gwgtptasql rrllglqcav gqvvtgdcge vevyespnwm anvtlklers
       61 pkdsflhivl twcdqyqngs nngaaagksg ppewwlasee leefcdpyst qiatehkdip
      121 vnafalgcfl gyntfvahfd stytmpppps plssakssss hsgksmsvkr gkkgrgakss
      181 cpsdgtsssa ktacpnselt spegfkvtqc nahfehcsng lylyfvdvrk rsstrfrllv
      241 rysticriil rktsptstiv ylqllhppll ykvcagnsat sqcnregdyl pqfdlsdttp
      301 wdravefgeg pfkcgadvlg rsrvmrltls gprhwaplcn lilhtrknaq vfyapvrelq
      361 arelkavarp svqdfgimya llsvwsesfq vtdelamdaq scretllela ahaeecpral
      421 eealfeiyfs lsqgkvfwfr salehlyfry rahrqridvf tgsviaqdlp shmvkvrkvi
      481 ltptrrvymp pqlvcksrll qhcdpdyalr ivvrdddckl isfslgackd dflsavikpp
      541 lssglkiggr rfmflgcsts qlrnhgiwfy ahdnqgrtae qirdgigdls tilsvpkfma
      601 rlgqafsqsl glltvprqnt svepdirhaa pgrssdgshr eyifsdgigr ishgllrkkk
      661 qeredakqwk krsidkeiet lrrkkkklea avaeltasad syaekagatr dltyimksns
      721 lrktakskqq eildidlkiq ekqneat
//
LOCUS       XP_077518299             275 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128629 [Amblyomma americanum].
ACCESSION   XP_077518299
VERSION     XP_077518299.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662173.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..275
                     /product="uncharacterized protein LOC144128629"
                     /calculated_mol_wt=30873
     Region          80..240
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..275
                     /gene="LOC144128629"
                     /coded_by="XM_077662173.1:210..1037"
                     /db_xref="GeneID:144128629"
ORIGIN      
        1 mpfedqillt lmrlrlglll ndlslpfgas taaisrifcf ivtklakfar eflvfwlprt
       61 airrtlpyvf eenysvtciv dcfeifidrp gqlhrrntty ssykshntak ilhviapsgf
      121 imflsqpygg rasdrfitld sgfldhltqg devladrgfn iddllppsvk vslpsfskgr
      181 pqicrsdvvs srclarlrih versirrlkc frilkqfpas iftkkpland vlvaivglcn
      241 lqpnlirdhg dasepgaqsp shpgkqlevv dntpe
//
LOCUS       XP_077518313             754 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128638 [Amblyomma americanum].
ACCESSION   XP_077518313
VERSION     XP_077518313.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662187.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..754
                     /product="uncharacterized protein LOC144128638"
                     /calculated_mol_wt=80784
     Region          664..747
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cl49608"
                     /db_xref="CDD:483948"
     Site            order(675..681,694,697,728,732,735..736)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467202"
     CDS             1..754
                     /gene="LOC144128638"
                     /coded_by="XM_077662187.1:16..2280"
                     /db_xref="GeneID:144128638"
ORIGIN      
        1 minrpwvkvl lrrqptavri vqssnvclra ptaaaacpia slcgdrpsvd sslkafmaql
       61 vrapspdsac eqdepwsshi pdaqrrippa gkasanqdsd gdsgidgsve rfshrissts
      121 saytepccnl pdssgarvvs vakrassval hdpslatqap gerrqdsapv sgrrppfskq
      181 smrrscrqlm aalyssstld prtagryrsf tlreqdakrl svphalpssg aklsvasaid
      241 gavapqwrss lasdcqlssl hnsaslpdha tgkhqassvr dvfkvpqgsa rrwatgtlek
      301 aprakcpffs alhergsrce rdadeywprt gawrfclggp cpclrkvsgg ranfgdetil
      361 rpedhpvcad dpqgcdrvqp avsshstasl sgpkttlppk alplslpgtp pkspsrcgpk
      421 saptmrvlfa rpasvvpdtp sassqrrgaa phcsrrgvae gnavsrpavg nsfedldeda
      481 srtqpsftll dlpkstslpt lvqqaadgrg gpgkggleys vlarkyhhav lspipritss
      541 pcvmddapla lprqsmhksd lalhsvgsgs saeratllgr vqelgrsars allrafster
      601 iyrpqkteta ardgpvglra rmslrkrrpt naapesscsd plwagghpth msaqllqqnl
      661 dgtqvvelrr ppgrsfgffl argrvhahhg vfvsrmpdsr tqqvlqglld igdeilevng
      721 tdvrdadime vnslmtnqst llltvlpyic rkdv
//
LOCUS       XP_077518339             108 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128649 [Amblyomma americanum].
ACCESSION   XP_077518339
VERSION     XP_077518339.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662213.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..108
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..108
                     /product="uncharacterized protein LOC144128649"
                     /calculated_mol_wt=12555
     CDS             1..108
                     /gene="LOC144128649"
                     /coded_by="XM_077662213.1:1..327"
                     /db_xref="GeneID:144128649"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedcrr rhlnrrltat mkrlsgvhvl
       61 nhehrflgas gepmlslfaa dryhvarrqg idpqcafvpl qwqvqvvs
//
LOCUS       XP_077518424             194 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128691 [Amblyomma americanum].
ACCESSION   XP_077518424
VERSION     XP_077518424.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662298.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..194
                     /product="uncharacterized protein LOC144128691"
                     /calculated_mol_wt=21958
     CDS             1..194
                     /gene="LOC144128691"
                     /coded_by="XM_077662298.1:1..585"
                     /db_xref="GeneID:144128691"
ORIGIN      
        1 mqfyssaeff qaiseekpal casalanflr dsnkkevlyy yvqmeleesr gnrgakeaaf
       61 lpllaalfmd dvgqlfrvfe egtsiterlh elpctptvva lgsffqnqcy iiceqqvlfs
      121 eptgfgeatr laflayyvfn msypvkaatt leflqreifd inptkgtkwn gkqkcrtivh
      181 arimklaanl rlnt
//
LOCUS       XP_077518461             251 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like [Amblyomma americanum].
ACCESSION   XP_077518461
VERSION     XP_077518461.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662335.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..251
                     /product="tubulin beta chain-like"
                     /calculated_mol_wt=28797
     Region          <8..251
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..251
                     /gene="LOC144128705"
                     /coded_by="XM_077662335.1:1..756"
                     /db_xref="GeneID:144128705"
ORIGIN      
        1 mpivsqpvhq lventyetyc idnealydic frtlklttpt ygdlnhlvfa tmsgvttclr
       61 fpgqlnadlr klavnmvpfp rlhffmtgfa pltsrgcqqy raltvpeltq qmfdaknmma
      121 acdprhgryl tvaavfrgrm smrevddqml niqnkdssyf vewipnnvkt avcdipprgl
      181 kmsatfigns taiqelfkri seqftamfrr kaflywytge gmdefeftea esnlnellse
      241 yqqyqeatpe d
//
LOCUS       XP_077518476             426 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077518476
VERSION     XP_077518476.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662350.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..426
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=47675
     Region          3..426
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..426
                     /gene="LOC144128711"
                     /coded_by="XM_077662350.1:1..1281"
                     /db_xref="GeneID:144128711"
ORIGIN      
        1 mlfwevisde hgidpsgayh gdsdlqleri nvyyneasgg kyvprailvd lepgavdavr
       61 sgpygrlfrp dnfvfgqsga gnnwakghyt egaelgdsvl dvvrkeaesc dclqgfqlth
      121 slgggtgsgm gtllfskire eypdrimnty svvpspkvsd tvvepynatl svhqlventd
      181 etycidneal ydicfrtlkl ttptygdlnh lvsatmsgvt tclrfpgqln adlrklavnm
      241 vpfprlhffm tgfapltarg cqqyraltvp eltqqmfdak nmmaacdprh gryltvaavf
      301 rgrmsmrevd dqmlniqnkd ssyfvewipn nvktavcdip prglkmsatf ignstaiqel
      361 fkriseqfta mfrrkaflhw ytgegmdefe fteaesnlne lvseyqqnqe atpedeeefe
      421 ktqaea
//
LOCUS       XP_077518498             106 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128726 [Amblyomma americanum].
ACCESSION   XP_077518498
VERSION     XP_077518498.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662372.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 18% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..106
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..106
                     /product="uncharacterized protein LOC144128726"
                     /calculated_mol_wt=11618
     CDS             1..106
                     /gene="LOC144128726"
                     /coded_by="XM_077662372.1:116..436"
                     /db_xref="GeneID:144128726"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrppfa altsvlccqr pasigpcvlp afgcvippdp
       61 fmlktkscme vvqatklrvi fardleqawe lvdgqcviyl mhklik
//
LOCUS       XP_077518545             333 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128757 [Amblyomma americanum].
ACCESSION   XP_077518545
VERSION     XP_077518545.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662419.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 37% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..333
                     /product="uncharacterized protein LOC144128757"
                     /calculated_mol_wt=37511
     Region          4..79
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          160..>330
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..333
                     /gene="LOC144128757"
                     /coded_by="XM_077662419.1:127..1128"
                     /db_xref="GeneID:144128757"
ORIGIN      
        1 mpgccvplca gsakkgarsf rfprdegrrk iweakvkren wkandnakic erhfeedqfe
       61 gnrmdgrrll kstavptlfd frpepkrrkp paprlfpgss tpdatkgssp tpettpdqee
      121 tlampentre dsgeqidifs lstselrkel qdekrkrsql eesllqankk lqkaakrtkq
      181 leenlttlta nlsflnqdqk aalekknrkn cswgantikk alqlkfacgs agydllleqg
      241 qpllsrrtlc rrlqhltflp gvlteiltlm eakvaavsei erdcvlflde leirrgveld
      301 rssdcflgki tlpesnqpan halvfmvggl ntr
//
LOCUS       XP_077518560             247 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128760 [Amblyomma americanum].
ACCESSION   XP_077518560
VERSION     XP_077518560.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662434.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 25% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..247
                     /product="uncharacterized protein LOC144128760"
                     /calculated_mol_wt=27501
     CDS             1..247
                     /gene="LOC144128760"
                     /coded_by="XM_077662434.1:234..977"
                     /db_xref="GeneID:144128760"
ORIGIN      
        1 maekseqdlt tssgivkfke lwnsnitchg infhvaqsge lekfkgyafl qrvwylqrvm
       61 agfrgkqwlv pyilagfyli stdyrqdrkn ltdalrdvgr sskvnllvln thsmkhadqi
      121 pyqllpgqrc vvtgptpmyg dkssdislvc snelqnrkhe skplvetalm tsipgtivaw
      181 esaagvldka gnmtkwlpkv elgfsvdhve ysyvaakaqf kcqnlsdypi vsavrnatgk
      241 ppleqig
//
LOCUS       XP_077518572             135 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128769 [Amblyomma americanum].
ACCESSION   XP_077518572
VERSION     XP_077518572.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662446.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..135
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..135
                     /product="uncharacterized protein LOC144128769"
                     /calculated_mol_wt=14276
     CDS             1..135
                     /gene="LOC144128769"
                     /coded_by="XM_077662446.1:11..418"
                     /db_xref="GeneID:144128769"
ORIGIN      
        1 maeepilsrs psvaaspeda psptsprdlh plskgpataw dtttatsped dsvptppqqe
       61 vrkpsedave pkeaftvgsk ndavgpavps lekdadqkkv sdimvsteeq qaasrrrtsi
      121 lrerpdihdg getlp
//
LOCUS       XP_077518586             268 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128777 [Amblyomma americanum].
ACCESSION   XP_077518586
VERSION     XP_077518586.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662460.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..268
                     /product="uncharacterized protein LOC144128777"
                     /calculated_mol_wt=29548
     CDS             1..268
                     /gene="LOC144128777"
                     /coded_by="XM_077662460.1:1..807"
                     /db_xref="GeneID:144128777"
ORIGIN      
        1 mydfavttsd trfrstfqay ccsqahsttv qikpslrlpi lvrtptgrqt iaqaekrllq
       61 ariletkcti krlendiyca qqfleklipa dffdvllhan segaknqelk hlvqnakfkq
      121 llasppeggv tasicklssh tldphersll lrglnfntga ppnavnmtca veeairkvap
      181 slqsearsra igalsklaks ktrtitpaeq taltrlqknk divilpadkg natvvldrtd
      241 yvrkmdalle dtttyaklsr gptkkies
//
LOCUS       XP_077518600             216 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128778 [Amblyomma americanum].
ACCESSION   XP_077518600
VERSION     XP_077518600.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662474.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 31% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..216
                     /product="uncharacterized protein LOC144128778"
                     /calculated_mol_wt=24336
     Region          <99..165
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..216
                     /gene="LOC144128778"
                     /coded_by="XM_077662474.1:1..651"
                     /db_xref="GeneID:144128778"
ORIGIN      
        1 msrctiring eaipyershs flgividrtl swspriydtr krlisiapvl sflegkswga
       61 svrsmlqryr slflgclrys lpifsairnt niqtlqgvqa ealricfglp kcaefisqep
      121 phdwivftds kaalqalqas lrpwnmgesc nlrlkaldpg lrlrlprnla rceaillcrl
      181 wigvaftnhy dfrmgmansp acescaceet iahllc
//
LOCUS       XP_077518637             263 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128795 [Amblyomma americanum].
ACCESSION   XP_077518637
VERSION     XP_077518637.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662511.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 34% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..263
                     /product="uncharacterized protein LOC144128795"
                     /calculated_mol_wt=29007
     Region          <88..132
                     /region_name="GT1"
                     /note="myb-like, SANT family; cl23759"
                     /db_xref="CDD:474047"
     Region          214..248
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..263
                     /gene="LOC144128795"
                     /coded_by="XM_077662511.1:1..792"
                     /db_xref="GeneID:144128795"
ORIGIN      
        1 mtssdattda rrgifwfvfr lkpctgwegs kadhsqnenv saaidkvfwe gdsatqavnl
       61 atprkaaqdl dltvgttnet gevasmavee crtrwrtird tylkrkqhrr anakgqwnvl
      121 drelgflddf lqprtrrwtl haaapvdsra eegakrkqpe edagereaat aeaaavaaav
      181 aeppeplava mrpaavlphh lapggaeaqe rcsdpeelfc lslaahlkrl lprernmakm
      241 kmlqtlhdle fgengeeaal eep
//
LOCUS       XP_077518650             311 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128801 [Amblyomma americanum].
ACCESSION   XP_077518650
VERSION     XP_077518650.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662524.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..311
                     /product="uncharacterized protein LOC144128801"
                     /calculated_mol_wt=35153
     Region          16..106
                     /region_name="THAP"
                     /note="The THAP domain is a putative DNA-binding domain
                     (DBD) and probably also binds a zinc ion; smart00980"
                     /db_xref="CDD:214951"
     Region          252..303
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     CDS             1..311
                     /gene="LOC144128801"
                     /coded_by="XM_077662524.1:80..1015"
                     /db_xref="GeneID:144128801"
ORIGIN      
        1 mqfartvyfd gvamvicavv gcsnrtcwak stntnffrlp kvikhqgdrt kalcakrrev
       61 wltrikradl ntertgirvc gahfvkgkps klweetdpdw tptlllsysa ihgdpgrhar
      121 aarqrtqkra aqaecqaees gtmeatnpdv vpaspssspr aehnvgvedc etgiavqtdl
      181 kmgdiealen ecvalnerly asrnenklle ltedalkcsd akvvfytgma nfrilyalfn
      241 vlerfvshnt nncllkfqef llfmmkfkln lhvtdlafrf nvsdatvsri fdkwlhtays
      301 rlkytmlcgl h
//
LOCUS       XP_077518663             216 aa            linear   INV 25-NOV-2025
DEFINITION  large ribosomal subunit protein uL1-like [Amblyomma americanum].
ACCESSION   XP_077518663
VERSION     XP_077518663.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662537.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 28% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..216
                     /product="large ribosomal subunit protein uL1-like"
                     /calculated_mol_wt=24010
     Region          1..216
                     /region_name="Ribosomal_L1"
                     /note="Ribosomal protein L1. The L1 protein, located near
                     the E-site of the ribosome, forms part of the L1 stalk
                     along with 23S rRNA. In bacteria and archaea, L1 functions
                     both as a ribosomal protein that binds rRNA, and as a
                     translation repressor that binds...; cl00322"
                     /db_xref="CDD:469720"
     CDS             1..216
                     /gene="LOC144128806"
                     /coded_by="XM_077662537.1:48..698"
                     /db_xref="GeneID:144128806"
ORIGIN      
        1 msikvsqdvl sdcmtsives asrqrrkrle nielwiklkd pdpdqhkrlf gkvkmkhail
       61 prsracivgd ekhceeaean nlncidlqal kniskstksi ralarvydif lvstllisqv
      121 nclmgyglsa ldkspinlth tqpisaqvdq vrttakirrk aghlvvavgn vkmtpenlva
      181 ntnqtvrcvt kllkkhgshv hsvcvrtimg pilkif
//
LOCUS       XP_077518709             535 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128830 [Amblyomma americanum].
ACCESSION   XP_077518709
VERSION     XP_077518709.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662583.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 14% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..535
                     /product="uncharacterized protein LOC144128830"
                     /calculated_mol_wt=60416
     CDS             1..535
                     /gene="LOC144128830"
                     /coded_by="XM_077662583.1:1..1608"
                     /db_xref="GeneID:144128830"
ORIGIN      
        1 mwhsapsnvf sisvnidglp lfkssctqvw pilvhvhhpr skqseiifpv gifcgtskpt
       61 cldeylkafn eemgallsng nlhksiaykv vlrailcdpp akafvkatkl fsgyyggdkc
      121 ctkgvylgri thqsmdsplr tdqafadqtq aehhkgispl tsigipmvtr fpldymhlvc
      181 lgvvrkligy wlrgplkvrl ahfyvvqlsg hlatmrqhit sdfarnprav dmferwkate
      241 frqfllysgp vvakkilprv myehflqlhv giailscssm dcfidyadql lrsfvskcks
      301 iygkeslfyn vhglihlane crqlgcldkf icfafenylk dlkahvrfgn kpleqlysri
      361 meqqacsvve nrssaefsay evlhsschss gpallypalk qyrkatwres virrkqpdnc
      421 lllsgnrivr asnfllsgse ilivgklckn egdfytrpcr ssllsvfnvs fdiveftdeg
      481 styvvpeswv kmeadgatcy wppttakkei drmillavpv eddwegckar ilatk
//
LOCUS       XP_077518723             447 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128837 [Amblyomma americanum].
ACCESSION   XP_077518723
VERSION     XP_077518723.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662597.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 22% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..447
                     /product="uncharacterized protein LOC144128837"
                     /calculated_mol_wt=49453
     Region          54..136
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     Region          275..349
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(289,291,293,318..320)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133137"
     Site            289..291
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133137"
     Site            289
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133137"
     Site            order(318..321,325..328)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133137"
     CDS             1..447
                     /gene="LOC144128837"
                     /coded_by="XM_077662597.1:272..1615"
                     /db_xref="GeneID:144128837"
ORIGIN      
        1 msrlgridey dpkvqnfdsy lerfehfvsa nevseakkls vfltvlgaea yevlknlvvp
       61 alpgektfae ikillknhys pqtsviaerc rfnrrvqleq esvedfvlel khlarkcnfg
      121 dflqdalrdr lvagirneet qralfttegl tfqgackial drelatqqaa llqqrgrsea
      181 lnavgtherc drktkdstrg ksqkqkcsrc gkahtsdscw ykrykcrrcs kvghlqnmcq
      241 asreelkahl vsesseeest lyscdatpsk ksyvvsvnve ghdlpmqvdt gaavtvvpes
      301 vyaaklshvk ceppklglkt ytgekmhvig qcnvtaryeg qcavlpivvr easlsqqasl
      361 pgeqprespg sagptvettc peetssagtn apasppspaa dstvpldasd dsresslpan
      421 ddavvpprrs qrmrrppdwf rpsdfkk
//
LOCUS       XP_077518735             105 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128844 [Amblyomma americanum].
ACCESSION   XP_077518735
VERSION     XP_077518735.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662609.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..105
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..105
                     /product="uncharacterized protein LOC144128844"
                     /calculated_mol_wt=11361
     CDS             1..105
                     /gene="LOC144128844"
                     /coded_by="XM_077662609.1:262..579"
                     /db_xref="GeneID:144128844"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrppfa altsvlccqr pasigpcvlp afgcvippdp
       61 fmlktkscme vvqatklrvi fardlvqagk lvdgqlysls kermh
//
LOCUS       XP_077518758             294 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128859 [Amblyomma americanum].
ACCESSION   XP_077518758
VERSION     XP_077518758.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662632.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..294
                     /product="uncharacterized protein LOC144128859"
                     /calculated_mol_wt=32767
     Region          165..>197
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          201..>263
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..294
                     /gene="LOC144128859"
                     /coded_by="XM_077662632.1:1..885"
                     /db_xref="GeneID:144128859"
ORIGIN      
        1 mtcmrapavl vdntnpdwap slhlgyrtrr vgsdrhdrsq qrrqrkrcse tsmtgseepc
       61 dvaapvpdvp ppphqaeevd ageetgvyvq tdmgmsdiga seqecirlsd cvytrrsekv
      121 qlemleeslr sselkvvfyt gmasftmlle ifkmlepfvs hsvnnslakf qefvlflmki
      181 klnlqnadla frfnvdvavi idcfeikier pssywarset wstykgsnta kyligiapqg
      241 ivtfisegwg grkmgictpd kvvsdrtnfq cssiipanla tvatvpivlv lhva
//
LOCUS       XP_077518790             126 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128885 [Amblyomma americanum].
ACCESSION   XP_077518790
VERSION     XP_077518790.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662664.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..126
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..126
                     /product="uncharacterized protein LOC144128885"
                     /calculated_mol_wt=14066
     CDS             1..126
                     /gene="LOC144128885"
                     /coded_by="XM_077662664.1:1..381"
                     /db_xref="GeneID:144128885"
ORIGIN      
        1 maganpeesv dlvqkrwksl cdkfcllyta rqlgrksgag addieptdfv wahyeqplfl
       61 rdtmvtrpkp lvccwlkwsl lgasgsedlr klqaaiqear skrgargkqy agvpaqqcpr
      121 airdhh
//
LOCUS       XP_077518815              69 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128899 [Amblyomma americanum].
ACCESSION   XP_077518815
VERSION     XP_077518815.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662689.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 25% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..69
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..69
                     /product="uncharacterized protein LOC144128899"
                     /calculated_mol_wt=7689
     CDS             1..69
                     /gene="LOC144128899"
                     /coded_by="XM_077662689.1:1..210"
                     /db_xref="GeneID:144128899"
ORIGIN      
        1 mavssclrpl ncvcyqeypa avqkqsglgc vteryrfapy hqcvlrmgrr lgknnrrplp
       61 scilkaltc
//
LOCUS       XP_077518839             242 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128915 [Amblyomma americanum].
ACCESSION   XP_077518839
VERSION     XP_077518839.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662713.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 23% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..242
                     /product="uncharacterized protein LOC144128915"
                     /calculated_mol_wt=27684
     Region          158..>206
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..242
                     /gene="LOC144128915"
                     /coded_by="XM_077662713.1:1..729"
                     /db_xref="GeneID:144128915"
ORIGIN      
        1 mtrltrlape rmrrrsahrl rrkrirtmyi reifdkrlrr vdpeydfkyf rmtkasfdrl
       61 lelihdkilh ppnhrrpisp gerlavtlrf lasgcsmqdi gmsycmhast vsqilketlp
      121 alwgilslvl krptaaqwen iqqaysikwn fphcigsidg khlaircpdh sgadfrnykr
      181 vfsivflgvv danyrfvpvd vgaegrfpna gsekrslsws sqaagvnssq splmlpagdc
      241 vl
//
LOCUS       XP_077518888             159 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128937 [Amblyomma americanum].
ACCESSION   XP_077518888
VERSION     XP_077518888.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662762.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 24% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..159
                     /product="uncharacterized protein LOC144128937"
                     /calculated_mol_wt=17489
     CDS             1..159
                     /gene="LOC144128937"
                     /coded_by="XM_077662762.1:1..480"
                     /db_xref="GeneID:144128937"
ORIGIN      
        1 mymaphealn agyyseligs irlryedklk lcddidpytl rpgvdtsadv sgfpeishgd
       61 ivtylvfsan fvtlenmkay kaleshnyft sgwvkhlsak efqdgkvvll gevnhsqrlg
      121 hkplqvwivc kkdgavitah ctcmagaggl pcwslfvcs
//
LOCUS       XP_077518925             166 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128959 [Amblyomma americanum].
ACCESSION   XP_077518925
VERSION     XP_077518925.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662799.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..166
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..166
                     /product="uncharacterized protein LOC144128959"
                     /calculated_mol_wt=17818
     Region          <5..124
                     /region_name="TesA"
                     /note="Lysophospholipase L1 or related esterase. Includes
                     spore coat protein LipC/YcsK [Cell cycle control, cell
                     division, chromosome partitioning, Lipid transport and
                     metabolism]; COG2755"
                     /db_xref="CDD:442045"
     CDS             1..166
                     /gene="LOC144128959"
                     /coded_by="XM_077662799.1:1..501"
                     /db_xref="GeneID:144128959"
ORIGIN      
        1 mesrilyvgg ndlarpnasp qaicvnvkal vllllsdvap vvlvakvlpr cfastdaeas
       61 lrllnrklta alkrmpcvgi lnpdhrflda vkapkrhlfa adgyhlsage gieelaevfv
      121 rglakfygag ilapfsgapa ryivhqchqc gskghrhggc yeycrp
//
LOCUS       XP_077518949             103 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128973 [Amblyomma americanum].
ACCESSION   XP_077518949
VERSION     XP_077518949.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662823.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 28% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..103
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..103
                     /product="uncharacterized protein LOC144128973"
                     /calculated_mol_wt=11900
     Region          <1..46
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..103
                     /gene="LOC144128973"
                     /coded_by="XM_077662823.1:120..431"
                     /db_xref="GeneID:144128973"
ORIGIN      
        1 manlgyhwtw qqlrncwknl kkrftaerle qersgaapsa wkwydhmsal lshrpmvqar
       61 eygvdsqdvp pdygdnsqld isdgdtrtee ddadilqeqs eqs
//
LOCUS       XP_077518971             190 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144128989 [Amblyomma americanum].
ACCESSION   XP_077518971
VERSION     XP_077518971.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662845.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..190
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..190
                     /product="uncharacterized protein LOC144128989"
                     /calculated_mol_wt=20891
     CDS             1..190
                     /gene="LOC144128989"
                     /coded_by="XM_077662845.1:602..1174"
                     /db_xref="GeneID:144128989"
ORIGIN      
        1 mmflrdtmtt rptsgnynre aqaatmsaqq dsspaqqtva esvfeleewr ldsesqasln
       61 dvvpvadidv vpvmdrlqas ldssassqsv qspqafvdlc aqsqasapps aqsapgkrnk
      121 rkrtaqdeel vghverltqa lsacdtlttr epedecelfa ltlakkmrrv paerqtqlqi
      181 kllevfqtfe
//
LOCUS       XP_077518995             153 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129003 [Amblyomma americanum].
ACCESSION   XP_077518995
VERSION     XP_077518995.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662869.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..153
                     /product="uncharacterized protein LOC144129003"
                     /calculated_mol_wt=17199
     CDS             1..153
                     /gene="LOC144129003"
                     /coded_by="XM_077662869.1:346..807"
                     /db_xref="GeneID:144129003"
ORIGIN      
        1 meaesaaava taalvilell eeppakkprt swvspwlqrr kqqgayenlm relaledsen
       61 yrrwirmdta tveellgevr phitkkdtpf reaiqagerl aitlrylatg nsemslqfaf
      121 yvahntisgi isevsqaiyr vpkpkvlkdg nqc
//
LOCUS       XP_077519004             518 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129011 [Amblyomma americanum].
ACCESSION   XP_077519004
VERSION     XP_077519004.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662878.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 41% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..518
                     /product="uncharacterized protein LOC144129011"
                     /calculated_mol_wt=58461
     Region          63..154
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     CDS             1..518
                     /gene="LOC144129011"
                     /coded_by="XM_077662878.1:1..1557"
                     /db_xref="GeneID:144129011"
ORIGIN      
        1 mpnnaplapq ppaaplvcsg tprqrdpavf sglgdsdved wlasyervst hnrwddamkl
       61 nnvifyladv anlwfrnhea diatwftfkt sfievfgrpg vrklraeqrl reraqqpget
      121 ftsyiedvvd lckrvnsdmt eadkiqnimk gidedafqml lakqpatvas vislcqsyee
      181 lrkqrvltrr avqpadvlsa dvetaggqql rhdihkfire elarqlalis gvrnpppals
      241 ptlqqtiraq vtealpplpq qpvaapltya avlarppshp lspfqdatqa pppstpppdf
      301 syraqmaqps prftpavshy gqrkqlkeke vtksrrmatg igclarqyhm vrredarpvv
      361 qpvrrvpfal leplreeldr meragivakv sertdwvcdp nlkiiavdpr cpgsnhdafs
      421 wryswlrsei eqvlledgef lleggdvdat dpddnepppa avytqgaasr itylrgwgrg
      481 gavrngiver frktrlqrla ylrtvrrqlr rqlqrrqh
//
LOCUS       XP_077519016             711 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129018 [Amblyomma americanum].
ACCESSION   XP_077519016
VERSION     XP_077519016.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662890.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..711
                     /product="uncharacterized protein LOC144129018"
                     /calculated_mol_wt=77900
     Region          421..673
                     /region_name="7tmB3_Methuselah-like"
                     /note="Methuselah-like subfamily B3, member of the class B
                     family of seven-transmembrane G protein-coupled receptors;
                     cd15039"
                     /db_xref="CDD:410632"
     Region          422..447
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410632"
     Site            order(427,431,473,476,479,487,494,579..580,582,584,639,
                     642,654,658)
                     /site_type="other"
                     /note="putative polypeptide ligand binding pocket
                     [polypeptide binding]"
                     /db_xref="CDD:410632"
     Region          456..477
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410632"
     Region          487..514
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410632"
     Region          530..550
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410632"
     Region          576..605
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410632"
     Region          620..647
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410632"
     Region          651..673
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410632"
     CDS             1..711
                     /gene="LOC144129018"
                     /coded_by="XM_077662890.1:1..2136"
                     /db_xref="GeneID:144129018"
ORIGIN      
        1 mtaplwclal fvtqvavlcq gqnmscaehm acpatavvha sgtrfarptc dcaplcvdyg
       61 sccldaslga splgppvmsc ravhladvss svymrtrcar dwkddevrre cedlaaetdp
      121 fltipvtsrs tnvtyknvrc avcnrdfnvk mwttflrsls dntvqacphf gdpsqcpvfl
      181 gcpdfmhgkr pqqyfrvcrs dlvsdcpadf hdrnsrlrdg chayfapvqd kltgvnyknf
      241 ycalcygvsa tdiacveegs vveypwiegd qseevshkdv lpsysllidv dfsrggakvg
      301 lqdrcapnqa ydpwkqtcrn lvcgklfelr dglcvasalr aaygssavsh allasncskv
      361 aldadeftlw enhtatlrad agtgellvpg eyelridgsi lvcaesgllp arrmavykfr
      421 dtdgyitlag sivslacllv kmalqicspg pwklpdllis mlslsifaaq llflfvmvaa
      481 rqralctlig mlvhyfflas flwtnalald lywsvasied ptkrrgghsf clfsaycwsv
      541 pcsvvvlsaa mdlwilptsh plspgygrgv cwisnrlall affaapiaai vavnmalfal
      601 cvvrisgtlt hadklrrgah trwrlvvyvk lalvmgatwt lgfvapfara qalwhafvil
      661 nslqgafvff aftrlalrhr laswlqvgrs kesssrrtiv saattvtvcs l
//
LOCUS       XP_077519042             166 aa            linear   INV 25-NOV-2025
DEFINITION  lysyl oxidase homolog 2B-like [Amblyomma americanum].
ACCESSION   XP_077519042
VERSION     XP_077519042.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662916.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 48% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..166
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..166
                     /product="lysyl oxidase homolog 2B-like"
                     /calculated_mol_wt=17918
     Region          47..>90
                     /region_name="Lysyl_oxidase"
                     /note="Lysyl oxidase; pfam01186"
                     /db_xref="CDD:460102"
     CDS             1..166
                     /gene="LOC144129033"
                     /coded_by="XM_077662916.1:2..502"
                     /db_xref="GeneID:144129033"
ORIGIN      
        1 mllsgvqcmg lqknladclh dqlgagavsc aglgesitgv vcvreqadlv ideheversa
       61 yledrqllyl qcameehcis asayalkadp evqqalgnpt atephamtya lqhtllspnd
      121 npyvfsemrs hffqnagfka ppaqpandvv mhngahgvhh fgiiye
//
LOCUS       XP_077519061             267 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129051 [Amblyomma americanum].
ACCESSION   XP_077519061
VERSION     XP_077519061.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662935.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 31% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..267
                     /product="uncharacterized protein LOC144129051"
                     /calculated_mol_wt=28418
     CDS             1..267
                     /gene="LOC144129051"
                     /coded_by="XM_077662935.1:1..804"
                     /db_xref="GeneID:144129051"
ORIGIN      
        1 mqrlsrleqa rqeprtrsps ppvsslppqc pqlpaftfed feaaeaavrd drvaaalrwa
       61 tanfsfqkqq llrlggnnlk evaanvmsav mgvavqrlfs lrgrkgkrpf vgtklcrvat
      121 gipdfellnh elgfpyacrk civlkvaflg lnahspctda icerqgvdil aaqgfisrwl
      181 pgvcdreggr krryaqaleh pesqapseep qaspvnpcag sahcpgapsa scpaappgma
      241 vmcpsgvhps sapspapppp pqpppha
//
LOCUS       XP_077519071             154 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129056 [Amblyomma americanum].
ACCESSION   XP_077519071
VERSION     XP_077519071.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662945.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..154
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..154
                     /product="uncharacterized protein LOC144129056"
                     /calculated_mol_wt=16431
     CDS             1..154
                     /gene="LOC144129056"
                     /coded_by="XM_077662945.1:12..476"
                     /db_xref="GeneID:144129056"
ORIGIN      
        1 mapaasvspa pspapqasqv mdehdycsvq enegqpgtra apppiqaaph qqggsirrph
       61 alpprqqdhp gsvqqrhnai aalieqqgrl atiqdqqlrq ittdlahirt hlagieslqv
      121 rqvnaieqlv qalrdinstl qgrnqapcen ggdp
//
LOCUS       XP_077519093             236 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129074 [Amblyomma americanum].
ACCESSION   XP_077519093
VERSION     XP_077519093.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662967.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 16% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..236
                     /product="uncharacterized protein LOC144129074"
                     /calculated_mol_wt=25515
     CDS             1..236
                     /gene="LOC144129074"
                     /coded_by="XM_077662967.1:1..711"
                     /db_xref="GeneID:144129074"
ORIGIN      
        1 mgtggappls plddslarve eavphvavrv pnsfdsdrps qspfsmpssq avtdiiggmg
       61 lgnaqpedsd defvmslgeg dqgafldgse lsspapplsh ggpstddtak rascsqtted
      121 graqegssae avarrrkcsr ltavdtalst elearllsae eernqrrieh errlqmikde
      181 hafktkhyav errkrsalhk mkvrvlkake qvqklkreip lkklqllapg gnaepe
//
LOCUS       XP_077519105             185 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129082 [Amblyomma americanum].
ACCESSION   XP_077519105
VERSION     XP_077519105.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077662979.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 45% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..185
                     /product="uncharacterized protein LOC144129082"
                     /calculated_mol_wt=20474
     Region          105..>161
                     /region_name="BEN"
                     /note="BEN domain; cl46346"
                     /db_xref="CDD:480686"
     CDS             1..185
                     /gene="LOC144129082"
                     /coded_by="XM_077662979.1:1..558"
                     /db_xref="GeneID:144129082"
ORIGIN      
        1 mlptaptyay vvykdggkai vaislikdca lsdvndvqkn kkvywrsevs greaeegyyd
       61 gdvvvlgrvl hrsactlgsv gskvqpiwvf saipakqpgv imqpqlmral wqekdlkdrs
      121 vtgkacnrkv qtgaqakpal tseklgavrd glreyirlhr vpnlldadrl rkmkqyiacl
      181 lrdvc
//
LOCUS       XP_077519164             414 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129116 [Amblyomma americanum].
ACCESSION   XP_077519164
VERSION     XP_077519164.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663038.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..414
                     /product="uncharacterized protein LOC144129116"
                     /calculated_mol_wt=43666
     Region          <201..>246
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     Region          240..>397
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..414
                     /gene="LOC144129116"
                     /coded_by="XM_077663038.1:18..1262"
                     /db_xref="GeneID:144129116"
ORIGIN      
        1 msshgaaiaa vagcgnrfas dadkdyeivl ptlptgrgvl ntvslhgdvr arpyrvedfr
       61 dalanagalp dvvalgayqi nhvwavtfst adaakklaat kelevkgrrc ivfdpedhqv
      121 klrlhwmlhg vadedirtaf aafgnvtevt rerwrvagmk ekgsttrtvl lklkpgvkvd
      181 dlphqvrvag elalvvvpgr pmqclrcggt ghvrrdckvp rcskcrrygh seadcvrtya
      241 satghvaasv saelmdvvea eeaasgaddt gkaegvpvqp avassntdta vaqesgssat
      301 dpsstpvetp kdgasssvhv ereaaseshd ekddsrmnvg vttpakrpha etkadgekaa
      361 epsvgeppak tpqarraglr prpkvpaekr ggdkappdqe hvlapddtgg dgiv
//
LOCUS       XP_077519188             325 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129131 [Amblyomma americanum].
ACCESSION   XP_077519188
VERSION     XP_077519188.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663062.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 12% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..325
                     /product="uncharacterized protein LOC144129131"
                     /calculated_mol_wt=36450
     Region          4..82
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          174..>311
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..325
                     /gene="LOC144129131"
                     /coded_by="XM_077663062.1:183..1160"
                     /db_xref="GeneID:144129131"
ORIGIN      
        1 mpgccafgcs nrtesgktff siptgkndre rrsawlhrig renfkptknt rvcedhfcae
       61 dfekarvdgk krlkhkavps ifshrvpkki rkppfarlpn spannlaeeq aepeeenlli
      121 aadsiadglk sskephdhde sadvatevpi tsdiqedapp nmfsevdvlk rqlnlegrkr
      181 araemecdal rssfngllae dqlrvlqkgt mrgsswtqkt iqeslkvrla cggrgyeylr
      241 gngwplpaer tlqkhiedik ftpgileevf palaskvatf hseeryavlm ldeiqltpgl
      301 dydittrsfi gtvwpipkel hagdv
//
LOCUS       XP_077519202             480 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129138 [Amblyomma americanum].
ACCESSION   XP_077519202
VERSION     XP_077519202.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663076.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 32% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..480
                     /product="uncharacterized protein LOC144129138"
                     /calculated_mol_wt=53053
     Region          40..107
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          263..465
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..480
                     /gene="LOC144129138"
                     /coded_by="XM_077663076.1:1..1443"
                     /db_xref="GeneID:144129138"
ORIGIN      
        1 maetlnalhv lhrtvssasf csflhvlrla wtafstapcc cafgcrtren dgkrlfrips
       61 anrdaarrki wlqrigradl iptqwsrlce dhftadsqel rgsavpscst pfespqdnsv
      121 agcmdadpts islqsslqda saiechsstl yiggvncyft iynlflccsd vdftcpllqn
      181 llqgiqtpst stsyealpat vtdvdesssk eafcaadeaa lhkriaeled elkshkrkls
      241 icqrqknaal qeksalkrrt chflangqlk smerytmrgt pwsastiekg lkirlscgsw
      301 gynvvreigt plpsertlhr hledlkflpg ilheilpsls vkvnlmkdhe khavlildem
      361 qltpgiaydq gtetvigkpt iplsngtlpd nvmamhglvf mlagvttrwk qtvayhltsn
      421 sfcsntvkhv ileiiksces igikidaivc dmgggnqalw kefrivvgrh crskcksspe
//
LOCUS       XP_077519215             219 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129145 [Amblyomma americanum].
ACCESSION   XP_077519215
VERSION     XP_077519215.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663089.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 32% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..219
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..219
                     /product="uncharacterized protein LOC144129145"
                     /calculated_mol_wt=24186
     CDS             1..219
                     /gene="LOC144129145"
                     /coded_by="XM_077663089.1:1..660"
                     /db_xref="GeneID:144129145"
ORIGIN      
        1 maaqercskl ameleappsr lvrpssqmpt cqtvtrswrq escpptrqpr slwkqkrksc
       61 vacgslppgg ggggdelaad enyprptrrt trasratrta sptrkeatdk esdcrtraqr
      121 prrvnksvav sedplddddd vacrgsvlvd dnsefsplkc pgemvvgmfs rssndgatas
      181 tyktrrhrll ppdldffeve deepppsark ttgrrrakh
//
LOCUS       XP_077519236             240 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129159 [Amblyomma americanum].
ACCESSION   XP_077519236
VERSION     XP_077519236.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663110.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..240
                     /product="uncharacterized protein LOC144129159"
                     /calculated_mol_wt=26325
     CDS             1..240
                     /gene="LOC144129159"
                     /coded_by="XM_077663110.1:773..1495"
                     /db_xref="GeneID:144129159"
ORIGIN      
        1 mgpggkglfq wlmpspteay platrpvdrd iallsvsfgt llglgafveh qivwrwlvkd
       61 hldatfqheq rslhvnlhln lcnacpqqva eyrlvvpyin vlkglinlkk ipamllgkvm
      121 ndyhvktgsp aaaaplrwlc swfltlgrph pqaghpgplp gvagcaltaq cprhpfllcc
      181 spnllphcdc hlqdpqdlga lgleglqhwa rvhnlaiqsw ttlvkmsinp litflpsphs
//
LOCUS       XP_077519250             213 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129164 [Amblyomma americanum].
ACCESSION   XP_077519250
VERSION     XP_077519250.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663124.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..213
                     /product="uncharacterized protein LOC144129164"
                     /calculated_mol_wt=23732
     CDS             1..213
                     /gene="LOC144129164"
                     /coded_by="XM_077663124.1:1..642"
                     /db_xref="GeneID:144129164"
ORIGIN      
        1 mtigferlrt tsyhpcangm ierfhrqska aimcqpdstw leatpavalg lhatfkpdir
       61 atpaeliyge plrrpgefla apqsstvtsd ltdfvarlrr titalrlspa ahhcktaffv
      121 fkdlatctht flcddtvrrp fqlpyrglyl vlcringndv hvsidrlkpa yidcaepgnt
      181 saptcvhppp tlqpasvtth ygrqvrytdf ykp
//
LOCUS       XP_077519276             616 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129171 [Amblyomma americanum].
ACCESSION   XP_077519276
VERSION     XP_077519276.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663150.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 18% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..616
                     /product="uncharacterized protein LOC144129171"
                     /calculated_mol_wt=69002
     Region          81..353
                     /region_name="Transposase_21"
                     /note="Transposase family tnp2; cl29371"
                     /db_xref="CDD:475188"
     CDS             1..616
                     /gene="LOC144129171"
                     /coded_by="XM_077663150.1:1..1851"
                     /db_xref="GeneID:144129171"
ORIGIN      
        1 msgetesddg ereflasdfv eleaailpgs tttkaaaiii imafvvthgl twealndllr
       61 lidglfgfkg dvlpqskfvf rklwssrker lvkrffycdt cgsvlvsvpg mssmrcpnce
      121 vstelcvlka rghffiildm keqmkcllak skaalferlv nlkaviqqgg aallqditsg
      181 smaeelrksg kigwmdltit intdgspvfk sssssvwpiq flinelppcd rlkncliggl
      241 wfgqhpymrt fltkfveein qfgkitwkag htllstrlhv lcccvdapar asvinmvqfn
      301 glfgcpwcyn caefhegaqr ymsvtggeer tpgemskdme faekikdavn glkgqsppnh
      361 lkhfnivfgh tveymlcvli gvtkcltdqw fdsansqqpf yigrpvtmak vdrrllaikp
      421 phsftrlprs lkdkcpwkas ewrhwllfya lpcclgilpq rylnhfallv eamftlllee
      481 ltlqqinhag rlledfvsrm palygarlmt fnvhqllhla kaaknfgplw ahsafvfetg
      541 ngrvlraits angapdqvie rmimlqqvdl amalctvqeq arnfictmlg hpltlnatrv
      601 gnsymfgacd pfphkv
//
LOCUS       XP_077519297             284 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129190 [Amblyomma americanum].
ACCESSION   XP_077519297
VERSION     XP_077519297.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663171.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 25% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..284
                     /product="uncharacterized protein LOC144129190"
                     /calculated_mol_wt=32330
     Region          99..253
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..284
                     /gene="LOC144129190"
                     /coded_by="XM_077663171.1:1..855"
                     /db_xref="GeneID:144129190"
ORIGIN      
        1 mdrglfrsyf rfeennlsrl qsalflpeii sapqgvsitg tealcitlhy risypecdnf
       61 vhllkdannh awldlrtlge fsqavyskaa plsngwgfid gtarafsrps tnqklffsgh
      121 krihaekyqa imcpngiiaq lngpytssrh dagilrksrt yqklenlvhr rsyclygdpa
      181 yplrplllkp yggaalnarq qafnkeisrv rqavecgfgk iaglfafldf rknqklypqn
      241 lprmyqvsai lancytcmye sqveqyfgle ppsletylvs qrhy
//
LOCUS       XP_077519310            1674 aa            linear   INV 25-NOV-2025
DEFINITION  hemicentin-1-like [Amblyomma americanum].
ACCESSION   XP_077519310
VERSION     XP_077519310.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663184.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1674
                     /product="hemicentin-1-like"
                     /calculated_mol_wt=179654
     Region          <51..229
                     /region_name="ChlD"
                     /note="vWFA (von Willebrand factor type A) domain of Mg
                     and Co chelatases [Coenzyme transport and metabolism];
                     COG1240"
                     /db_xref="CDD:440853"
     Region          449..528
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          459..463
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          472..476
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          508..513
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          535..606
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          633..706
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          641..645
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          654..658
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          686..691
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          699..702
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          709..789
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          806..892
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          824..828
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          837..841
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          859..862
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          872..877
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          885..888
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          905..984
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          915..919
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          928..932
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          950..954
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          964..969
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          977..980
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          999..1082
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1006..1010
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409405"
     Region          1019..1023
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409405"
     Region          1048..1052
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409405"
     Region          1062..1067
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409405"
     Region          1075..1078
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409405"
     Region          1096..1176
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1106..1110
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1119..1123
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1142..1146
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1156..1161
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1169..1172
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1185..1270
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1199..1203
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1212..1216
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1234..1240
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1250..1255
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1263..1266
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1273..1366
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1293..1297
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1306..1310
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1330..1337
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1346..1351
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1359..1362
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1468..1563
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1492..1496
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1505..1509
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1528..1533
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1543..1548
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1556..1559
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1566..1645
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     CDS             1..1674
                     /gene="LOC144129199"
                     /coded_by="XM_077663184.1:384..5408"
                     /db_xref="GeneID:144129199"
ORIGIN      
        1 mareqrflsv lypsavcvaf llcvqmgats rtfsdvgdgw aendselegg aapdnavslv
       61 fvldvsgsmh rhlqqvadgl qsilnaslsq kvfynyvlvp fhdpvvgppl vtrdpghfeq
      121 rlrslslrgg gdcpemalta lrealrlslr gsqvylftda rakdyhllaa vlafvqlkes
      181 qvvffltgdc gdptqpglva fqqiaaassg hvfrlretdv kevanfvkas mqpdkvslvs
      241 vdarsqdihl lemnidstvr elmvsisger pqlvlinpqg rslaagadmd valdvanala
      301 vvvrapaagr wqmrvssadg rhtvratgvs eagfrhgfsr qpvegmrhtq rtplsgspay
      361 vlvngssssi vlrelhlvsv sggqlaklvl npvsrratly nastsfvppd dffflkvvgt
      421 dkagypvnrl tptalraepp dipevvtrqr llvatganat lrcsirsllp avaqwqrdgs
      481 plagpqrlrg sfqfplhisr vsledagtyl csasndigvs ssavlleikv asvapqlrls
      541 paevhfrtgd lvrvtceake gsptpsvrws vspplrhrvd vngstvtlhd aregdelqel
      601 aciasnaags dvrrpslqyv dgpkitvreg avvtvakgss rtlacfasgt pkpqvrwskd
      661 gsevtkglqp dgslliddva eadagdytcd atstagsdsy tvrvhvqlpp qilasasqdv
      721 yqvvenaafs ldcrslgspa pklewykdgr lvdaavghlg itfsaqqddl rvaaaklihd
      781 gvykcvasna agsterrfrv ivllppqiqg pprevvrvve gqevtlscra egtpapsatf
      841 lrdgvswpsd ergdgyrely fatamaerda gvyvcratne agsaqklfel vvlvppaied
      901 hetlgvvrvr vgskavlrca atgvpspevs wllgasrisg adgrlrpgas elvietarqe
      961 dagryrcsav nevgtasref lvdvlvppgf ksggrvswel legepvtfdc tatgnpapdv
     1021 vwfkgsseii pgaawllqgg tdvirgghal smfhvvreha gpyscaaine lgratrnfsl
     1081 svlvrptmea gpsqrhqlqv akgnsvalfc nvtglpspvv rwlrddqpvq fragvslfqs
     1141 grglnissie lsdagkymci gtnnvgsask efqvevqerp sinssslerh qtallhqpvt
     1201 lacpasgspp pelswfhdgv pllesdpkll ffqggrlvtl qpalpssagi ytceavnavg
     1261 rasidyilev lvppsiptrd letrlkvveg aqttlrcpaq grpaptivwm rgpelvanda
     1321 ddtrievptg dqqslvirrv qlgdrrftcl agnaagtaei sfvldvmvrp kmepgdeepv
     1381 navvavlnrp awlrcpvvgv pppsvrwlra grllnhrggd pfvqpsadgr rlhlrrarlr
     1441 dagnftcvai neagklekhf tvdvqvpphi sslpssapda aesvhvvand tlsllcrvta
     1501 qpkasvvwlk esvplkaegs rhqispdset ltidhvqvsd rgkytcvain nagtdekdfh
     1561 vsvlvpptis gpavvdvntv enlpitiscd vdslplatvy wtknslpyii qnsesdvtsk
     1621 ygarvlsfpa vqssdrgiys ciaeneagla ekmfrievqg kstnisadhy vsta
//
LOCUS       XP_077519321            2227 aa            linear   INV 25-NOV-2025
DEFINITION  hemicentin-1-like [Amblyomma americanum].
ACCESSION   XP_077519321
VERSION     XP_077519321.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663195.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2227
                     /product="hemicentin-1-like"
                     /calculated_mol_wt=237209
     Region          15..92
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          109..198
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          126..130
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          140..144
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          164..168
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          178..183
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          191..194
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          218..292
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          221..225
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          234..238
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          259..262
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          272..277
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          309..400
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          316..320
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          329..333
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          364..370
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          380..385
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          393..396
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          403..497
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          423..427
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          436..440
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          463..467
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          477..482
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          490..493
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          498..599
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          521..525
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          538..542
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          565..569
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          579..584
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          592..595
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          611..683
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          621..625
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          634..637
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          649..653
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          663..668
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          676..679
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          698..777
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          706..710
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          719..723
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          743..747
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          757..762
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          770..773
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          789..868
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          800..804
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          813..817
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          840..843
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          848..853
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          861..864
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          884..967
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          892..896
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          905..909
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          931..935
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          945..950
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          960..963
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          968..1043
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          1068..1143
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          1079..1083
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1092..1096
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1109..1113
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1123..1128
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1136..1139
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1146..1219
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          1236..1322
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1253..1257
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1266..1270
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1288..1292
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1302..1307
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1315..1318
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1325..1404
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          1429..1507
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          1439..1443
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1452..1456
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1473..1477
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1487..1492
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1500..1503
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1511..1596
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1528..1532
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1541..1545
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1565..1569
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1579..1584
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1592..1595
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1603..1690
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1620..1624
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1633..1637
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1656..1660
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1670..1675
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1683..1686
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1701..1781
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          1711..1715
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1724..1728
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1747..1751
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1761..1766
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1774..1777
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1792..1845
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1851..1903
                     /region_name="TSP1"
                     /note="Thrombospondin type 1 repeats; smart00209"
                     /db_xref="CDD:214559"
     Region          1908..1961
                     /region_name="TSP1"
                     /note="Thrombospondin type 1 repeats; smart00209"
                     /db_xref="CDD:214559"
     Region          2034..2062
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     Region          2064..2100
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(2064,2067,2083)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     CDS             1..2227
                     /gene="LOC144129205"
                     /coded_by="XM_077663195.1:1..6684"
                     /db_xref="GeneID:144129205"
ORIGIN      
        1 mlllmgspti aadlpprirv pekalsvseh spvklrcias gepqpsvvwm rddspldkrd
       61 skdvafaeng qilqipnatl ahigkyscva rndagmaeeq illsvlappr itklqppapv
      121 vlgrparlec rvhqgspras vewtkdgell verqpllqva dggqvvhvvr atehmegaya
      181 cratnaagsd ehtlflsvlv pprlladekq hvwrlaqgsd vqmecfvfgr paprivwykd
      241 nqevsvrpgr vsllqdnqil yltgvtrmea grylctaenv agnvtadfdv hvqsppeieg
      301 afdeatvfsa rlgeslelsc aaagdpdpvv awvregdasq fyssvpsssp tspdplvrvr
      361 ghgshvvlel krlrasdaga ytciatssag vaekrflvnv svpasivkps nglelsvalg
      421 esatleclvt gfpapslrwk kdgaplfans lqdsgftfls ggqtllvhat tasdagvytc
      481 datnaegsde ihyvlsviep pdfgaktgtt nvlmarqgdd vtvrcpfivk dtdssavstt
      541 wrldgrpmal dslpanlala agdaselrvg hvqpedagyy tctasntage asftsflhvl
      601 vppyfeeada eevyvvegga vslecfprgi pepavswlls enklpvesaf lrvpnasaed
      661 aghyvcqasn dagvaekdfi lvvlvppsfn atdnsvavtv legeearlac pasgvpdprr
      721 lwyregrrrv ypgpsmqlsd dgrvltlspa rlhdggrftc vafseagtae lnitltvyvp
      781 psirpaadpg pvvavrghpa rlaceatgvp aptvswfktd ahgfarvpvg tgpfldftdg
      841 istdhegcyi claeneagsq ersvvlnvla pptvertdta evsvmavagh naslsctaag
      901 spqpdiswlr rgalagkhek rlltsvgkga stltildvea tdagaylcva tngggvaave
      961 yridvvvpph vsstnataee vvlaqhpfsl pcdadgdplp siawyheglp vprdslttvr
     1021 agwlhvsaak rhhaghyvce asnvggavri aynvtvwerp qiigpdspvr lsavlgesvq
     1081 lqcsatgtpt pilswsndgr aleafnates vtlevvreed agiyactaan qagvttremv
     1141 ltvlvppqir rqagsnltev vagrpavlec aadgfprprv swsgpaplad avtwtahgav
     1201 riarvgkeha gnytctatne agsdtmsvql svlsapsvlr stdnvvvvag qpailwcnnt
     1261 adpkpdvtwr kddhglgddn esfeildgal yiysanvtds gryvctvhnh agstqavrnl
     1321 dvldppvitl fypehkvvvl geeislicqa kghpkptisw ehknvlises ntsidqryif
     1381 vtggglkipv aksrdageyr csarsqagwd srtttvavhv aphieddvse vsvqrggvav
     1441 lsckahgqpp plvtwtkegd pvdsprarhe aggnlviaga epedsgsyvc saeneaglrq
     1501 rlvalevlvp psistlprdq evaagkplrl gcralgsptp ritwthngsp vpkrtyspva
     1561 edgaselvls nvapgdqgsy tcvaengagq hkatatvvvg vvpsiaetps hsqvasgkel
     1621 rlnctvkgdp apklywlrgg hplddedgri qrldngslrv shiqvddage yhclasnkfg
     1681 maqvnvtvav ltppsfhltt kgvvvrenet atldcaasgq ptptyvwrrv ghslvedggr
     1741 wsafangtlr vtsaqasdgg lyrceatnav gtasaevslt vkagieeeva wgpwgpcsas
     1801 cgpglkerlc vfvasgqpla aetsspsstc aertrnvvac fdsacpvhgg wgswsewssc
     1861 sascgggtrt rrracdsppp esggrdcqge peqedfchpe acpvhggwse wspwgecsat
     1921 cgrrrhrrrf rtcdnpspsa ggrgclgsdy etrrcppkpc pqgglhpsal mavkapfgts
     1981 tgdlrnntli tgwlqelraa ppigfirews cpegylpvrg acedddecsn ydqcqheckn
     2041 tpgsyecvcp pgyrldadgy tchdvdecqk awpdcgpdha cfntrggyec vhvpcppnye
     2101 raaeenscrl rcsltedcvr gssdvvtyws valpsdvrat spvarlvard hdgavlgnta
     2161 ftslsgdtqp fdvsdergvg vvfatrdlrp rtkyrvtlha vsrddansta lfttrffllv
     2221 ttgnypf
//
LOCUS       XP_077519328             594 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: putative ATP-dependent RNA helicase DDX43
            [Amblyomma americanum].
ACCESSION   XP_077519328
VERSION     XP_077519328.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663202.1
KEYWORDS    RefSeq; corrected model; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio            :: 12% of CDS bases
            internal stop codons :: corrected 2 genomic stop codon
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..594
                     /product="LOW QUALITY PROTEIN: putative ATP-dependent RNA
                     helicase DDX43"
                     /calculated_mol_wt=66270
     Region          75..144
                     /region_name="KH_1"
                     /note="KH domain; pfam00013"
                     /db_xref="CDD:459630"
     Region          266..594
                     /region_name="SrmB"
                     /note="Superfamily II DNA and RNA helicase [Replication,
                     recombination and repair]; COG0513"
                     /db_xref="CDD:440279"
     CDS             1..594
                     /gene="LOC144129216"
                     /coded_by="XM_077663202.1:1..1785"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: substituted 2 bases at 2
                     genomic stop codons"
                     /db_xref="GeneID:144129216"
ORIGIN      
        1 magavtgdie paplvmsrtv dpapvqfeer iwieeeyvpp ppqqayeapr fggrsnrsgg
       61 gdrrdrngtd anantevmvv ppkdigniig kggskirqlq dssgarirvl rdegdgsyns
      121 fyeakiafsr pdearhkaqq likeliyppe txsmxqsrea dssedqprpl idwerliaks
      181 dkeremhsaa fapviknlys edpniacmsr aqveafrlen nnivvkylvp dgipppdvat
      241 npvttfeqaf anypeifdei yknkleklsp iqsqawpill qgrdligiaq tgtgktmafl
      301 lpamihidsq pvpreertgp sclvlaptre laqqierear kyhyrgikcv riygggsrre
      361 qiqtvnlgae iviatpgrln dlvmnkiidl nyvtfllsst tfqahvldea estldmgfep
      421 qikkvlldir pgrqtvmtsa twpegvrrlg qqyttnpfqv figsldlaav htvmrtiiic
      481 detkkflqlk gfldsmppen kvivfvdrka mvdhlssdfi legincdsih gsreqcdreq
      541 alhdfrdgkv riliatdvaa rgldikdvth kfnydfprnv eeyvhqvgrt grag
//
LOCUS       XP_077519342             270 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129221 [Amblyomma americanum].
ACCESSION   XP_077519342
VERSION     XP_077519342.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663216.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 49% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..270
                     /product="uncharacterized protein LOC144129221"
                     /calculated_mol_wt=29880
     CDS             1..270
                     /gene="LOC144129221"
                     /coded_by="XM_077663216.1:332..1144"
                     /db_xref="GeneID:144129221"
ORIGIN      
        1 mnrmdavvpa ilrarnssqa rdpthrppla altsvlccqr pasigpyvlp afgcvippdp
       61 fmlktkshme vvqatklrvi fardleqagk lvdgqwysls kasffsktkt iflgswamyl
      121 gtcgqdnagq hasqsskkst kqrsfqmqrt fqtmrekktg veteatrass qrvvydimtg
      181 qllavssefq hqrdrellar legashysst mqqakqalpi fpqriprtrn klttgahqln
      241 fccqskfgaf vnfqptckam laqgageqva
//
LOCUS       XP_077519366             680 aa            linear   INV 25-NOV-2025
DEFINITION  microtubule-associated serine/threonine-protein kinase 2-like
            [Amblyomma americanum].
ACCESSION   XP_077519366
VERSION     XP_077519366.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663240.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 11% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..680
                     /product="microtubule-associated serine/threonine-protein
                     kinase 2-like"
                     /calculated_mol_wt=77014
     Region          26..299
                     /region_name="S_TKc"
                     /note="Serine/Threonine protein kinases, catalytic domain;
                     smart00220"
                     /db_xref="CDD:214567"
     Site            order(32..35,38,40,55,84,100..103,146,150..151,153,
                     163..164)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     Region          455..524
                     /region_name="PDZ"
                     /note="Domain present in PSD-95, Dlg, and ZO-1/2;
                     smart00228"
                     /db_xref="CDD:214570"
     Site            order(455..461,473,476,505,509,512..513)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467153"
     Region          <474..616
                     /region_name="degP_htrA_DO"
                     /note="periplasmic serine protease, Do/DeqQ family;
                     TIGR02037"
                     /db_xref="CDD:273938"
     Region          <603..640
                     /region_name="PDZ"
                     /note="Domain present in PSD-95, Dlg, and ZO-1/2;
                     smart00228"
                     /db_xref="CDD:214570"
     CDS             1..680
                     /gene="LOC144129234"
                     /coded_by="XM_077663240.1:1..2043"
                     /db_xref="GeneID:144129234"
ORIGIN      
        1 mrtsflrtss sapppfwafi pkmrdfetlk ilgsggfgvv hlaafkpail mttvklvnme
       61 rftrqkqaam dkilasvvsn pfivkyyccf vckqayvtvm eyvpgvdlmr lvnherylei
      121 davkvimaql ilalehmhlr gflhrdvkvs nmllmpggrl klidfdttkv chglfsrrim
      181 rsffrktpfe fndaesagtv pymapevlrr rpygrsvdww sagivmyklm tgrvpfrgkt
      241 kqalreriit aplrwprpee rehsattpak dmtfrmlrkn averlgsrsy vdlqthpffd
      301 rfnwkrlrhe qhlceipslv plirsirsrd tidtttasdk rrllklgdlk dvsadgqrpl
      361 lcyasdsfkk lilaverane piavsdsfme ttletepeis yaplpapppi sadllssfsp
      421 slpedgassp vsvlvgaeki dlvlyrkkry skywgfgfal qrlrgednin yfvdvkpdsp
      481 aeksqllvld fllkvngetv ldmpmgrvkt liaasgdqml ltvmsaspyr altmrrdmma
      541 vlrgapresv vlypvratya lqksygvell epdvwddrgk qfvkvyvvka anvnpgnqps
      601 lypgdvistv dgtpvelmtr eqlyrmlnga rsqlpvtvvp lsplrsahrv hisklretti
      661 sdttlvtrln aaeydneesd
//
LOCUS       XP_077519379             167 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129241 [Amblyomma americanum].
ACCESSION   XP_077519379
VERSION     XP_077519379.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663253.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 11% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..167
                     /product="uncharacterized protein LOC144129241"
                     /calculated_mol_wt=18628
     CDS             1..167
                     /gene="LOC144129241"
                     /coded_by="XM_077663253.1:255..758"
                     /db_xref="GeneID:144129241"
ORIGIN      
        1 mlspqspqst aspqpvekaa varrtkksak tlstlfprip ktaatlleef lrresrvsly
       61 vtplltfard qlclhaedcl lklrkqvity qdvrnmvenl iglqkmcfkr epemakplgi
      121 slrkliiivg evasslekms dvpvtdwvai geevvkkhek vnqcals
//
LOCUS       XP_077519414             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129272 [Amblyomma americanum].
ACCESSION   XP_077519414
VERSION     XP_077519414.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663288.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 28% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144129272"
                     /calculated_mol_wt=19756
     CDS             1..181
                     /gene="LOC144129272"
                     /coded_by="XM_077663288.1:280..825"
                     /db_xref="GeneID:144129272"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrppfg altsvlccqr lasigpcvlp afgcvippdl
       61 fmlktkscme vvqatklrii fardleqagk lvdgqwysls kasffsktkt iflgswamhl
      121 gtcgqdnagq htsasskkkh etlpwsggcl mqipsflcgt rdrddgrkar mnltralhpd
      181 h
//
LOCUS       XP_077519426             436 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129281 [Amblyomma americanum].
ACCESSION   XP_077519426
VERSION     XP_077519426.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663300.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..436
                     /product="uncharacterized protein LOC144129281"
                     /calculated_mol_wt=48608
     CDS             1..436
                     /gene="LOC144129281"
                     /coded_by="XM_077663300.1:271..1581"
                     /db_xref="GeneID:144129281"
ORIGIN      
        1 mdsehgveee kpgpvlfdpk rlfhyseeew sriwwrsqld gvyshatttk hvstdmksdg
       61 lapaspsavk rtlrpipepg pststeapka vvelpdqrws nscaqvthlq gvkdasnmds
      121 eygaaeekpg pvlfdpkrlf hysekewsli wwrsqldgig sqatttkhvs tdmksdglap
      181 aspseakrtl rpipepgptt steapkaavg fpeqsranac aeqallqgfk rschmdsehh
      241 tgqeqldwqs pgvdifmkvt dapatltreq iwsvlrtvwy svakypsmtr laaayfaril
      301 kkehpgifld fllgacysrf nltlptiatv yytgdhwitl lhflgelvva mpqddmrrdl
      361 qelrtvlaiq fcaccyella spvayytsya qrilevvvra gkdieelaph smqalaahat
      421 araaemksln leelce
//
LOCUS       XP_077519552             128 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129354 [Amblyomma americanum].
ACCESSION   XP_077519552
VERSION     XP_077519552.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663426.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..128
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..128
                     /product="uncharacterized protein LOC144129354"
                     /calculated_mol_wt=14550
     CDS             1..128
                     /gene="LOC144129354"
                     /coded_by="XM_077663426.1:1..387"
                     /db_xref="GeneID:144129354"
ORIGIN      
        1 mtarppefdd ttgswssyrv rleaffeghg ftdpgkkral lvsalsdnvv rvlqgqcqse
       61 svnslsyevv iqhldnhfdp kaneiaasya ffmrnqaege kvrdyitdlr hlakdcsfek
      121 fldrmlrd
//
LOCUS       XP_077519583             203 aa            linear   INV 25-NOV-2025
DEFINITION  disabled homolog 1-like [Amblyomma americanum].
ACCESSION   XP_077519583
VERSION     XP_077519583.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663457.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..203
                     /product="disabled homolog 1-like"
                     /calculated_mol_wt=22169
     Region          30..166
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     CDS             1..203
                     /gene="LOC144129379"
                     /coded_by="XM_077663457.1:1..612"
                     /db_xref="GeneID:144129379"
ORIGIN      
        1 mtsrfstwlg garwsnrggs pkmdqegsla gnpsrfegeg llfrakligr qavpdghgyr
       61 vcqdamaklk aavrasrqhk rrvhlslslq girirdeksg ellfhhpvqq imfisrdtgd
      121 araigyiyma qdrsfhyiai kteraaaelg malvglfqvv leilqqgrql pgdvqeaiss
      181 ppdvgsaepp vpetipaveg lle
//
LOCUS       XP_077519648             597 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129398 [Amblyomma americanum].
ACCESSION   XP_077519648
VERSION     XP_077519648.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663522.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..597
                     /product="uncharacterized protein LOC144129398"
                     /calculated_mol_wt=66692
     Region          125..>151
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            134..136
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133137"
     Site            134
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133137"
     Region          413..468
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     CDS             1..597
                     /gene="LOC144129398"
                     /coded_by="XM_077663522.1:715..2508"
                     /db_xref="GeneID:144129398"
ORIGIN      
        1 mlpwpkllkc velgntaksk vkkysskncq vevnavlqmk srcnkcarvh nsetcpakgr
       61 tclkcgkrhh faaacktksa nlcakeqkva sleansdgqa dnfwlqtlsp sskkddrwta
      121 ivkieetpva ckldtgancs visrsqlqki tqkqpencav vlntffgfqk katkrshlhv
      181 tgpegtfett ffvmeddipv tlsgsvaekl glirriasle hqelydvvkp yedvfsglge
      241 lkgvvyhlkl kpgsqgvvka arripfaled svkaeldrme aagvvsnvte ptewsshmvt
      301 vikndkesvk ylghvltrdg lsldpgrled ifhvqapcnr kelqtflasd rrleqvlret
      361 drdpsmvklr qyastawpid kadvttelrs ywsfrdelht esglvfrsnr liiparlrne
      421 vlaslhaaht gaekmkarar tvvfwpsins dieevaqrcq tcqkyksrna rlpllsheip
      481 tlpwesigad ichhngaeyl vvvdfysfff eirlvqapsa nkviaacndi fathgfprrl
      541 ctdngppfss qqfkdyadki glqhdqmntr vsvsadvlse aekdatfads staeeet
//
LOCUS       XP_077519663             187 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129404 [Amblyomma americanum].
ACCESSION   XP_077519663
VERSION     XP_077519663.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663537.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 23% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="uncharacterized protein LOC144129404"
                     /calculated_mol_wt=19304
     CDS             1..187
                     /gene="LOC144129404"
                     /coded_by="XM_077663537.1:87..650"
                     /db_xref="GeneID:144129404"
ORIGIN      
        1 maaahkqkga gtaedpelhi edaevgvvne gatglcavse gdvwpasagv ncgqhshggr
       61 hrdtggsans ysgptpesgr snagedgckc spcstlpsly dghhretgrp eagcqqmvll
      121 lalqavslph wsrpcpfsal kscescdffr klsaafddqe galsigpiww syralasgvs
      181 lgsialp
//
LOCUS       XP_077519727             293 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129431 [Amblyomma americanum].
ACCESSION   XP_077519727
VERSION     XP_077519727.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663601.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..293
                     /product="uncharacterized protein LOC144129431"
                     /calculated_mol_wt=33196
     Region          126..265
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     CDS             1..293
                     /gene="LOC144129431"
                     /coded_by="XM_077663601.1:1..882"
                     /db_xref="GeneID:144129431"
ORIGIN      
        1 mehickkrkv lrtqvtklik eclerldrlt qsdasvlhar lmslhaelna vnsrfeeild
       61 ddqaeteyeq ifeyndrivt vlaklqqcis nptgnppttq hennaaqatt tskirlklpr
      121 leltrfsgrr hdwqpfwevf eqvvdkneel ssvdkfhylr asvtgdaaaa laglpptarc
      181 ysdavellkk rfgneelliq ehmkklidiq pvrssddvrg lrrlydtlaa hikgletlgq
      241 khdsyssllm pivqrasptd illdysrqcl iattsssded eesptddase scn
//
LOCUS       XP_077519738             156 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129438, partial [Amblyomma
            americanum].
ACCESSION   XP_077519738
VERSION     XP_077519738.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663612.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..156
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         <1..156
                     /product="uncharacterized protein LOC144129438"
     CDS             1..156
                     /gene="LOC144129438"
                     /coded_by="XM_077663612.1:<1..471"
                     /db_xref="GeneID:144129438"
ORIGIN      
        1 tstecsilfh iagflvkgil kainnceqck atllgssdae nayltrlkey lengdnlhyp
       61 spevmkvlks ceehfkgite waeslltmks pvqavitylr qmvpfnvetc qvhneiiqkl
      121 lystyartrl rihlrqleek kvnghasktc agvslp
//
LOCUS       XP_077519758             164 aa            linear   INV 25-NOV-2025
DEFINITION  histone H2A.V-like [Amblyomma americanum].
ACCESSION   XP_077519758
VERSION     XP_077519758.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663632.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 23% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..164
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..164
                     /product="histone H2A.V-like"
                     /calculated_mol_wt=17770
     Region          21..149
                     /region_name="PTZ00017"
                     /note="histone H2A; Provisional"
                     /db_xref="CDD:185399"
     CDS             1..164
                     /gene="LOC144129447"
                     /coded_by="XM_077663632.1:1..495"
                     /db_xref="GeneID:144129447"
ORIGIN      
        1 mvseetlqap earvtegtma dgksgkdlgk akaksvsrsa raglqafpcg rihrhlkyrt
       61 ighdrvgata avysaavley ltaevlelag iaskdmkvkh itprylqlai rgdeeldfli
      121 katiaggdvv phihkyligk kgsqktpkpy tslsqcsyrq grff
//
LOCUS       XP_077519765             334 aa            linear   INV 25-NOV-2025
DEFINITION  MICOS complex subunit Mic60-like [Amblyomma americanum].
ACCESSION   XP_077519765
VERSION     XP_077519765.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663639.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 20% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..334
                     /product="MICOS complex subunit Mic60-like"
                     /calculated_mol_wt=38439
     Region          <25..315
                     /region_name="Mitofilin"
                     /note="Mitochondrial inner membrane protein; pfam09731"
                     /db_xref="CDD:430783"
     CDS             1..334
                     /gene="LOC144129457"
                     /coded_by="XM_077663639.1:1..1005"
                     /db_xref="GeneID:144129457"
ORIGIN      
        1 mvayksdakf caflkmssaw nqmteqerae nfqrqqerma rnldrqrael aavlevkqqe
       61 afehelqqqr rrqvaarteh lkealeeqke elsrtfalel enkmaeeraq hnaalcssva
      121 klqgmdeylr ardelyrlsq qakglwlacq glrhtlvhgp aggpqslstd vqlveraapm
      181 spyvplvlts ilkealawgv ypevalkarf prveracqrv alvdykfpss llrylasylq
      241 slciiypklp keeltdeqrv npemwdtfdv lsrvrywlqh knleqalrya sqlrgqprlv
      301 aqdwihelrl hlevrmaasa lmafaaasia edae
//
LOCUS       XP_077519774             476 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129473 [Amblyomma americanum].
ACCESSION   XP_077519774
VERSION     XP_077519774.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663648.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 12% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..476
                     /product="uncharacterized protein LOC144129473"
                     /calculated_mol_wt=53624
     CDS             1..476
                     /gene="LOC144129473"
                     /coded_by="XM_077663648.1:1..1431"
                     /db_xref="GeneID:144129473"
ORIGIN      
        1 mssapieclv twegrkqvlf vggdrkksdv inaamladsw aslqcdriev ynsrfdtyvd
       61 vpeghtfsdg dkinlvgsss sdeqnlplet gtddgplvla pscsepdkrl ftvqqplyel
      121 ppipldikad iegttlgevs srtknrivsw itahlltidw ypgkfyepaa kalvlkypvl
      181 rdtigtgyds wrvafkykmg nirksvdnea rkkfgrkgnf qdksglkkqc rvprdtlett
      241 veevdgvvaa hvdfmakemk lcnpdiqklk vsmqttlpsr rkwininsps teelldkypa
      301 ltlremihqe fmeickvdfe rkllgfingh gqrlfsilkt srtaqkifse vqkaiadseg
      361 dakkycfavg vlqllphlvk eeafylrrpd sypcivmndk phtatnvcat fenmkveaid
      421 visgfatvme iywvmnieys kknkntfavl ehfcglkskp lkpvtlraia llekan
//
LOCUS       XP_077519785             524 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129492 [Amblyomma americanum].
ACCESSION   XP_077519785
VERSION     XP_077519785.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663659.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 43% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..524
                     /product="uncharacterized protein LOC144129492"
                     /calculated_mol_wt=58856
     Region          190..363
                     /region_name="RT_LTR"
                     /note="Reverse transcriptases (RTs) from retrotransposons
                     and retroviruses which have long terminal repeats (LTRs)
                     in their DNA copies but not in their RNA template. RT
                     catalyzes DNA replication from an RNA template, and is
                     responsible for the replication of...; cd01647"
                     /db_xref="CDD:238825"
     Site            order(254..259,288..289,314,316..317,359..360)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238825"
     Site            order(254..259,288,316)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238825"
     Site            289
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238825"
     CDS             1..524
                     /gene="LOC144129492"
                     /coded_by="XM_077663659.1:1..1575"
                     /db_xref="GeneID:144129492"
ORIGIN      
        1 mahhqlpeyd dqsdnwkayi tkveayfeat gvsdsgkkra llvaalstrt vqvlsgqvap
       61 rkpnsltyee avkvldayfd pkrheitesy rffnrcqled epiqqfivev riadgcnfgd
      121 mldrmlrdri vcgeaaippp cipelgliyg hpvhlklkeg nmpkfckars lpydlrdkvs
      181 keidrlvtlg vlspvthaew atpivpvmkn gevricvdfk vtlnpvcele qypfpaiddi
      241 faclnggdcf stldlrdayn qvpldedsrk tcvinthrgl fcynrlpfgi asapaifqrk
      301 ietvlqgltg vkaylddvlv seklgstdrl rqvlqrfleh gvklrlekcs frqeavtylg
      361 hkidreglhp iekhvgaiaq apnpqnvqhd swplplpeat vyarnfgrgk kwtpgivest
      421 tgsrvvsirt pagsvrrhvd qvrnredatp pfgkdqeaak rdsrtgtpsd ypllprtpae
      481 qggitepehe lqpelagsna epvrspqalr rstrqrkpvq rlhy
//
LOCUS       XP_077519795             691 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129508 [Amblyomma americanum].
ACCESSION   XP_077519795
VERSION     XP_077519795.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663669.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..691
                     /product="uncharacterized protein LOC144129508"
                     /calculated_mol_wt=77685
     Region          <224..682
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..691
                     /gene="LOC144129508"
                     /coded_by="XM_077663669.1:1..2076"
                     /db_xref="GeneID:144129508"
ORIGIN      
        1 mqdmgaltrg mdqedittsr vkqaclatca aiavavtvva fyylfrasps hwprqlicvt
       61 ddcrlhalfl dyninnsfsp cadfdghict twmpsqtyte lgatalgqva vnwvdnflpl
      121 lrraadpiki aekplemyea cvkerddata adaarfaafl kevnlvwper sgtnalglli
      181 nmafnwqmtf wlsfrwrivs ekhlkffivs gnsdvlllfa mnhryvmqrd syleywmshy
      241 ttlygntslp parqiktsar eqtdilsvlt dvgkkkrslv tvvpvgnsan fsgritssdw
      301 leqlkarlpt eqfppsgdqv lgdstlleai gllfnkysde qlvyhlswqf iqlyfltlqr
      361 tplqivhwgk tyssayvpvy ctlfveevfr pllsalhikm aiapsdtarl nfalggliek
      421 lvstvnssql epgpkraama ryqsmqmqiw ppgkyfndeg tekayscypt nrdsfvqywi
      481 eshrclqrta gtafhressg mhrllssspv tydpiinela vaasalahpl yyphgtpamf
      541 ygglgfliva ealkaldrps lavlsnasaa lsnsttgssk eprcftvlen dtratyfaal
      601 niaysafved eanefqerrr pisrehseet vfflticrmm crartlqnag vincnalfks
      661 cpyfanafac ppespmnpae rcfsfqtnrd d
//
LOCUS       XP_077519803             449 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129518 [Amblyomma americanum].
ACCESSION   XP_077519803
VERSION     XP_077519803.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663677.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 37% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..449
                     /product="uncharacterized protein LOC144129518"
                     /calculated_mol_wt=50084
     Region          <215..>317
                     /region_name="PRK03918"
                     /note="DNA double-strand break repair ATPase Rad50"
                     /db_xref="CDD:235175"
     CDS             1..449
                     /gene="LOC144129518"
                     /coded_by="XM_077663677.1:1..1350"
                     /db_xref="GeneID:144129518"
ORIGIN      
        1 msnpllfymq fflfgttsvs alqdsaclvi tipatygvca yepphhddrw tsmrpgpsts
       61 srayirsqpp stpssghvgw ppdsmsnpll fymqfflfgt tsvsalqdsa clvitipaty
      121 gvsayepphh ddrwtsmrpg pstssrayir sqppstpssg hvgwppdsms npllfymqvs
      181 nksstvkssd rcfiqltcpp ilctlclvvr dfvfsllmls gdvepnpgpt teemlaeiln
      241 gqrkiekrle eiemklkvve esasavkeis kkvsglektv rslqdkfidl edrsrrnnll
      301 vfgvkekdee tqddlkeavi egvfentlgv qvssverihr igrkqgtrpr pviiklfdhr
      361 ekmtilkncs klknkafsvs edfsahtrlk rkklwdstse iraaggkvql vydkiringe
      421 lflwdsasng riptsrptvd ahnppsppq
//
LOCUS       XP_077519810             235 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129529 [Amblyomma americanum].
ACCESSION   XP_077519810
VERSION     XP_077519810.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663684.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..235
                     /product="uncharacterized protein LOC144129529"
                     /calculated_mol_wt=26257
     Region          <6..185
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..235
                     /gene="LOC144129529"
                     /coded_by="XM_077663684.1:171..878"
                     /db_xref="GeneID:144129529"
ORIGIN      
        1 mryddewile cmlmrmrnpk lyehlrrqsi mvlpgrtclk kylqrfkggf glstkvfdal
       61 nektktmday srhgglvide iklsehlnvk sagdiegfvd lgghtpadqk nvladhgmiv
      121 lfqpftgkwt qilgvfaskg nvkaptlaki ilettvlaek aglfvdcitc dgaswnrsmw
      181 rlfgiqgsps hvrsstkhpv dpkrqlyfls dfplllkrta avaewlsirl acgsg
//
LOCUS       XP_077519817             240 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129539 [Amblyomma americanum].
ACCESSION   XP_077519817
VERSION     XP_077519817.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663691.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..240
                     /product="uncharacterized protein LOC144129539"
                     /calculated_mol_wt=26223
     Region          <4..37
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..240
                     /gene="LOC144129539"
                     /coded_by="XM_077663691.1:69..791"
                     /db_xref="GeneID:144129539"
ORIGIN      
        1 maaariqrwa vylggynykl qyvpgkqlln sdalsrltqq ttrdggegpt egshdwtrpp
       61 dsslyvrnyg qgekwipghv ksatgarmvt iepptaivkr hvdqvrrrsd sspripvtdt
      121 targpgprqt kgpstaanvt tpleaaapde spdtaqiagn niqpltsllp ptlrpaeasq
      181 qavrrstrqr kpvqrfnfgg egrvvfvmle ccacakhfns ffplplpftl qvikaitrpk
//
LOCUS       XP_077519823             346 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129550 [Amblyomma americanum].
ACCESSION   XP_077519823
VERSION     XP_077519823.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663697.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..346
                     /product="uncharacterized protein LOC144129550"
                     /calculated_mol_wt=36950
     Region          <142..203
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..346
                     /gene="LOC144129550"
                     /coded_by="XM_077663697.1:352..1392"
                     /db_xref="GeneID:144129550"
ORIGIN      
        1 mlprepgplr ggeevqpged saacaawrdr gdqpcscccc sgaclwrqqq npqtsggppv
       61 vhdnmvgpfe rgtgvpddlh glnfkpasyg svptqdpkap rggdgptcda vtvvehcpcg
      121 dhrgvddmhk hsddatgtaa agreftlvtd dqplvsplrp drqtptmtaa riqrwalylg
      181 gynyklqyvp gkqllnsdal srltqqttrd rgegptegsh dwtrppdssl yvrnygqgek
      241 wipghvksat garmvtvepp taivkrqvdq vrrrsdsspr fpvtdttarg pgpsqtkgpc
      301 taanvttple aaapdyspdt aqiagnniqp rtsllpptls paeasq
//
LOCUS       XP_077519830             445 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129558 [Amblyomma americanum].
ACCESSION   XP_077519830
VERSION     XP_077519830.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663704.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 44% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..445
                     /product="uncharacterized protein LOC144129558"
                     /calculated_mol_wt=47803
     Region          <242..302
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..445
                     /gene="LOC144129558"
                     /coded_by="XM_077663704.1:116..1453"
                     /db_xref="GeneID:144129558"
ORIGIN      
        1 mlprepgplr ggeevqpged saacaawrdr gdqpcscccc sgawlwrqqq npqtsggppv
       61 vhdnmvgpfe rgtgvpddlh glnfkpasyg svptqdpkap rggdgptcda vtvvehcpcg
      121 dhrgvddmhk hsddatgtaa agdltlrvgf rdvlvdcplm vfdcegpslc grdlltlqdn
      181 agapvlhltp acgatetset dtckiksifs dyqdvfttel glmkgppasl rlkdeaipkf
      241 creftlvtdd qplvsplrpd rqtptmtaar iqrwalylgg ynyklqyvpg kqllnsdals
      301 rltqqttrdr gegptegshd wtrppdssly vrnygqgekw ipghvksatg armvtveppt
      361 aiikrqvdqv rhrsdssprf pvtdttargp gpsqtkgpct aanvttplea aapdyspdra
      421 qiagnniqpr tsllpptlsp aeasq
//
LOCUS       XP_077519835             282 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129568 [Amblyomma americanum].
ACCESSION   XP_077519835
VERSION     XP_077519835.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663709.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 23% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..282
                     /product="uncharacterized protein LOC144129568"
                     /calculated_mol_wt=30184
     Region          <78..139
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..282
                     /gene="LOC144129568"
                     /coded_by="XM_077663709.1:1..849"
                     /db_xref="GeneID:144129568"
ORIGIN      
        1 mvgpfergtg vpddlhglnf kpasygsvpt qdpkaprggd gptcdavtvv ehcpcgdhrg
       61 vddmhkysdd atgtaaagre ftlvtddqpl vsplrpdrqt ptmtaariqr walylggyny
      121 klqyvpgkql lnsdalsrlt qqttrdrgeg ptegshdwtr ppdsslyvrn ygqgekwipg
      181 hvksatgarm vtvepptaiv krqvdqvrrr sdssprfpvt dttargpgps qtkgpctaan
      241 vttpleaaap dyspdtaqia gnniqprtsl lpptlspaea sq
//
LOCUS       XP_077519839             346 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129575 [Amblyomma americanum].
ACCESSION   XP_077519839
VERSION     XP_077519839.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663713.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 18% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..346
                     /product="uncharacterized protein LOC144129575"
                     /calculated_mol_wt=36951
     Region          <142..203
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..346
                     /gene="LOC144129575"
                     /coded_by="XM_077663713.1:157..1197"
                     /db_xref="GeneID:144129575"
ORIGIN      
        1 mlprepgplr ggeevqpged saacaawrdr gdqpcscccc sgaclwrqqq npqtsggppv
       61 vhdnmvgpfe rgtgvpddlh glnfkpasyg svptqdpkap rggdgptcda vtvvehcpcg
      121 dhrgvddmhk hsddatgtaa agreftlvtd dqplvsplrp drqtptmtaa riqrwalylg
      181 gynyklqyvp gkqllnsdal srltqqttrd rgegptegsh dwtrppdssl yvrnygqgek
      241 wipghvksat garmvtvepp taivkrqvdq vrrrsdsspr fpvtdttarg pgpsqtkgpc
      301 taanvttple aaapdyspdt aeiagnniqp rtsllpptls paeasq
//
LOCUS       XP_077519854             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129603 [Amblyomma americanum].
ACCESSION   XP_077519854
VERSION     XP_077519854.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663728.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 16% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144129603"
                     /calculated_mol_wt=15953
     Region          16..140
                     /region_name="Tudor_SF"
                     /note="Tudor domain superfamily; cl02573"
                     /db_xref="CDD:470623"
     Site            order(69,72,75,94,97,99)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:410449"
     CDS             1..141
                     /gene="LOC144129603"
                     /coded_by="XM_077663728.1:229..654"
                     /db_xref="GeneID:144129603"
ORIGIN      
        1 mgpklpesgc igcchlshir gdgliwvqis gpgittlnkf tyavkeyykn nngtvdkpef
       61 gkiyarqyrv dltfyratlt srdtlpsgef qlrfvdfgke dqaylselrd ldnlgeffvr
      121 llkqgcdvlc kakplrcdik f
//
LOCUS       XP_077519867             112 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129619 [Amblyomma americanum].
ACCESSION   XP_077519867
VERSION     XP_077519867.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663741.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..112
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..112
                     /product="uncharacterized protein LOC144129619"
                     /calculated_mol_wt=12195
     CDS             1..112
                     /gene="LOC144129619"
                     /coded_by="XM_077663741.1:324..662"
                     /db_xref="GeneID:144129619"
ORIGIN      
        1 maavvpailr arnssqardp thrppfaalt svlccqrpas igpcvlpafg cvippdpfkl
       61 ktkscmevvq atklrvifar dleqagklvd gqwyslskas fffkysfwhg ia
//
LOCUS       XP_077519878             505 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129631 [Amblyomma americanum].
ACCESSION   XP_077519878
VERSION     XP_077519878.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663752.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 23% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..505
                     /product="uncharacterized protein LOC144129631"
                     /calculated_mol_wt=56672
     Region          165..253
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     CDS             1..505
                     /gene="LOC144129631"
                     /coded_by="XM_077663752.1:1..1518"
                     /db_xref="GeneID:144129631"
ORIGIN      
        1 mraariltpc vltrqfalss sgvvpgstpw itprcvpcap atanitsits pgtwtgswyh
       61 aandaasdvs lsssdatstl atglmqrsis tepcprstdf vaekivaeat smaapvspiv
      121 lqqprqpptf rgstfedpes wletfervat fnswasddkl rhvyfameda artwfenrea
      181 tlttwnlfrs sfmqtftsvl rkervqalle traqlpneai aifteemsrl frhadpemse
      241 ekklrllmrg vkeelfcami rsppttveef lreatsiekt lemrnrpfnr ranstnyagv
      301 qllatddlre tiravvreel qrifpssqpq vasvaeivke vqrslgvpev qpqssqphpe
      361 amtyaavarr qgppprprqd pvtpqfrrpp pppptrppvd rcsyprktdv wrapdhrplc
      421 yhcgeaghvy rrcpyrdlgl rgfavdaprp rvgerprdia dylaatrwsp rrpsrspspg
      481 rylsppcrpy tgpargrsas pypen
//
LOCUS       XP_077519883             156 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129639 [Amblyomma americanum].
ACCESSION   XP_077519883
VERSION     XP_077519883.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663757.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..156
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..156
                     /product="uncharacterized protein LOC144129639"
                     /calculated_mol_wt=17811
     CDS             1..156
                     /gene="LOC144129639"
                     /coded_by="XM_077663757.1:1..471"
                     /db_xref="GeneID:144129639"
ORIGIN      
        1 mtetggpste qnshpqdvas lrlrlpsfwp qnpqvsflqi etlfslhrit sevtryyqvv
       61 stlppnhlkt kvlerlmlse harlqqllta edpgdrrpfq llrwirqllg erdvpyytap
      121 lrepflnclp qpirlvltaa gdvtldrtae lanfvl
//
LOCUS       XP_077519890             139 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129649 [Amblyomma americanum].
ACCESSION   XP_077519890
VERSION     XP_077519890.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663764.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..139
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..139
                     /product="uncharacterized protein LOC144129649"
                     /calculated_mol_wt=15657
     CDS             1..139
                     /gene="LOC144129649"
                     /coded_by="XM_077663764.1:44..463"
                     /db_xref="GeneID:144129649"
ORIGIN      
        1 mascyqklqs allykavkdi scgdssscgt hqqsqcnsnc fmkhiqrtvl rcenqiifse
       61 asdfteaact afilyyimnq eypegaaisl kfvqrqfdln lpnesrqkdv krsrtsvpsk
      121 imnlvnklgl qvisrrfal
//
LOCUS       XP_077519900             200 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129666 [Amblyomma americanum].
ACCESSION   XP_077519900
VERSION     XP_077519900.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663774.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..200
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..200
                     /product="uncharacterized protein LOC144129666"
                     /calculated_mol_wt=22372
     Region          <8..62
                     /region_name="transpos_IS481"
                     /note="IS481 family transposase; NF033577"
                     /db_xref="CDD:468094"
     CDS             1..200
                     /gene="LOC144129666"
                     /coded_by="XM_077663774.1:1..603"
                     /db_xref="GeneID:144129666"
ORIGIN      
        1 msrfgcsdtv ttdrrrqyec tlfaaidril garhsrttky hpcaigmver lhrqlkaala
       61 srlncahwvd slplgllglr avlradlqcs aaellyassl rlpadfsisa qlpaqpqdfl
      121 qrlvdnakdl rpilprphhh ntiivhrdla tsthvkfvcr davrapltsa ydgpfrvlrr
      181 tlktatlfqn gheecqygpp
//
LOCUS       XP_077519912             275 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129682 [Amblyomma americanum].
ACCESSION   XP_077519912
VERSION     XP_077519912.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663786.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 20% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..275
                     /product="uncharacterized protein LOC144129682"
                     /calculated_mol_wt=31064
     Region          80..240
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..275
                     /gene="LOC144129682"
                     /coded_by="XM_077663786.1:204..1031"
                     /db_xref="GeneID:144129682"
ORIGIN      
        1 mpfedqillt lmwlrlglll ndlslrfgas taaisrifcf ivtklakfsr eflvfwlprt
       61 airrtlpyvf eenysvtciv dcfeifidrp gqlhrrntty ssykshntak ilhviapsgf
      121 imflskqygd rasdrfitld sgfldhltqg devladrgfn iddllppsvk vslpsfskgr
      181 pqicrsdvvs srrlarlrih versirrlkc fkilkqfpas iftkkpland vlvaiaglcn
      241 lqpnlirdhg dasepgaqsp shpgkqlevv dntpe
//
LOCUS       XP_077519955             384 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129742 [Amblyomma americanum].
ACCESSION   XP_077519955
VERSION     XP_077519955.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663829.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 20% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..384
                     /product="uncharacterized protein LOC144129742"
                     /calculated_mol_wt=43252
     Region          155..>251
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     CDS             1..384
                     /gene="LOC144129742"
                     /coded_by="XM_077663829.1:1..1155"
                     /db_xref="GeneID:144129742"
ORIGIN      
        1 manrypdgip lktidsiqva eglvevfsry gfpreifsdr gsnftselmk evnrllqlet
       61 pspstsspap afkqlrppas lcglggcatm rslnpfclam qvskpcclya kkssdyfliq
      121 lpspqcclai vtecsdviis lillpgdiet npspasletv vtelkklsag qstliaevtd
      181 lknqllttdn lisdlsrris aleghyqtiq slrteiegls thttkatrql cdledridea
      241 enqsrrnnli fynipdpdps etwadsekll irhcsefldi tldpkridrt hrlgrhepdr
      301 crpliakfal fktkdeilan grkfkntdys vgedfsrrvr nvrkhlvtfa ksktnrfslr
      361 yktlfigsrr yifdepsqsv keip
//
LOCUS       XP_077519974             170 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129770 [Amblyomma americanum].
ACCESSION   XP_077519974
VERSION     XP_077519974.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663848.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 39% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..170
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..170
                     /product="uncharacterized protein LOC144129770"
                     /calculated_mol_wt=18481
     CDS             1..170
                     /gene="LOC144129770"
                     /coded_by="XM_077663848.1:517..1029"
                     /db_xref="GeneID:144129770"
ORIGIN      
        1 mhavfkggae dgarntpspn edptaqeaed sltasltslg slkekatmks prrclqlldt
       61 irghlqdtep deevitwlep llektaerln iasahlpqss vqankklths dglyrrnanv
      121 qqsalkdgse yvptvrvlgi qtdvvppepl pvtrfalwgm lssalrcadr
//
LOCUS       XP_077519979             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129779, partial [Amblyomma
            americanum].
ACCESSION   XP_077519979
VERSION     XP_077519979.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663853.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         <1..181
                     /product="uncharacterized protein LOC144129779"
     Region          11..170
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..181
                     /gene="LOC144129779"
                     /coded_by="XM_077663853.1:<1..546"
                     /db_xref="GeneID:144129779"
ORIGIN      
        1 qgkysatrvi ldvteikcqa asslalqsat fssykpantf kgligispdg tvtvvsnlfp
       61 gsisdkecvs ksgflalpfn dgdvvmadkg fkieemleti nvalnnppfv rrgqfsgeei
      121 keteeiaslr ihverriqri ksihifdrlv plslgpiine mwivcgiitn fqsplvqlsd
      181 e
//
LOCUS       XP_077520006             124 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129808 [Amblyomma americanum].
ACCESSION   XP_077520006
VERSION     XP_077520006.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663880.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..124
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..124
                     /product="uncharacterized protein LOC144129808"
                     /calculated_mol_wt=13168
     CDS             1..124
                     /gene="LOC144129808"
                     /coded_by="XM_077663880.1:542..916"
                     /db_xref="GeneID:144129808"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrppfa altsvlccqr pasigpcvlp afgcvippdp
       61 fmlktkscme vaqatklrvi fardleqagk lvdgqwysls kasffsgavr rggqtlsvep
      121 spgp
//
LOCUS       XP_077520030             341 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129836 [Amblyomma americanum].
ACCESSION   XP_077520030
VERSION     XP_077520030.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663904.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..341
                     /product="uncharacterized protein LOC144129836"
                     /calculated_mol_wt=36541
     Region          66..193
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     Site            order(70..73,102..104,107,132,171,189)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260008"
     Region          <252..288
                     /region_name="zf-RVT"
                     /note="zinc-binding in reverse transcriptase; pfam13966"
                     /db_xref="CDD:433612"
     CDS             1..341
                     /gene="LOC144129836"
                     /coded_by="XM_077663904.1:1..1026"
                     /db_xref="GeneID:144129836"
ORIGIN      
        1 mgllystfhr lfgppvnqap fpppptrppl pisvdirscs rrrtpvaalq qtaatllqes
       61 lgghlqvytd gsvlpatgqa aaacvapalg aalschlrfp anstaaelag lhlaadllll
      121 lpgqqpavil tdsraalqll rqhqprqhtv anlkarlmai qdagrpvslq wlpshvgitg
      181 neaadqlaga ahtngspvss kvtqldfarp alrqavralh pdprvasgvr fmrvpdclpr
      241 reralilrlr tgcswtqarl hrhgrapspa csfcgadetl ghllcacpnl eaarrdmtag
      301 yrnlglpsys dqdllhpkrs qteafrllle flqstglatr l
//
LOCUS       XP_077520040             356 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129845 [Amblyomma americanum].
ACCESSION   XP_077520040
VERSION     XP_077520040.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663914.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..356
                     /product="uncharacterized protein LOC144129845"
                     /calculated_mol_wt=39855
     Region          56..117
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     Region          271..>345
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     CDS             1..356
                     /gene="LOC144129845"
                     /coded_by="XM_077663914.1:1..1071"
                     /db_xref="GeneID:144129845"
ORIGIN      
        1 matrgfpnqi qefngsswvg rlqfyfeann itdpalkran lltlcgeqty dtvcaliqps
       61 tpaavsyddi vaalqkhydp rpsevysrar fqrrdqlege tvsayiaelk rlaahcnfgt
      121 littatgqer daassanptm lpldvmlrdr fvcglcdesl qqrmfaekdl tfnkayitvr
      181 aesaghqqre irrntepvhq tsnqtpsqdv vqqlqekyei vfseeipgne gppvtlellg
      241 naqpkflkar pvpfalrtsv ekeldkleqq gvieptqhsq watplvvvrk kngticlcgd
      301 ywstvnlatk assyplptpe evfsifrgar vfstldltqa yqqlkdrlra asssws
//
LOCUS       XP_077520057             275 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129866 [Amblyomma americanum].
ACCESSION   XP_077520057
VERSION     XP_077520057.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663931.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..275
                     /product="uncharacterized protein LOC144129866"
                     /calculated_mol_wt=29666
     CDS             1..275
                     /gene="LOC144129866"
                     /coded_by="XM_077663931.1:1..828"
                     /db_xref="GeneID:144129866"
ORIGIN      
        1 magrrkerai vlhdpalaha asplsggyta arrrelwqql aallnaagpa aetvlqwrht
       61 wqvwctrckk qeaqflaagr gtgggrlpgl agrvcklrsp glvqgvaapm yssssqnqpr
      121 vsvgempgts glqqaearsp satqeqrdvv agrpcqqqrs twaeevhqll aetrrtndll
      181 earsaedatf rvrlleeqrq tsravrslta avtqltaait etgahmvrta daaradtvrl
      241 teqvvlaval ivrvlnnqtf pipasspala cwrrg
//
LOCUS       XP_077520082             306 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129893 [Amblyomma americanum].
ACCESSION   XP_077520082
VERSION     XP_077520082.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663956.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..306
                     /product="uncharacterized protein LOC144129893"
                     /calculated_mol_wt=35527
     Region          <63..>192
                     /region_name="COG2433"
                     /note="Possible nuclease of RNase H fold, RuvC/YqgF family
                     [General function prediction only]"
                     /db_xref="CDD:441980"
     Region          117..>165
                     /region_name="HOOK"
                     /note="HOOK protein coiled-coil region; pfam05622"
                     /db_xref="CDD:461694"
     CDS             1..306
                     /gene="LOC144129893"
                     /coded_by="XM_077663956.1:160..1080"
                     /db_xref="GeneID:144129893"
ORIGIN      
        1 mpldehcgsc dmqleeldsk idcsdcrsvy htgkcagisd atikskgeqy rsawrcsncr
       61 rskgrtpqld rssndtddqd vrallksine kleyllplke tvdsvedtvq ymsdqydell
      121 trveknerev relkhrvdki ekqddeitql knemdclewr srklnlefhg iksmkdenli
      181 deinklatln nlpqlqesdi vsthrlpskq dkipgiicrf akqsvrdkww qnrktlcqgn
      241 dsifvlenlt krtrtlltet klwakannyk yvwhnnnkvl vrkadgqnav viscssdrdk
      301 lkqqis
//
LOCUS       XP_077520091             250 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129903 [Amblyomma americanum].
ACCESSION   XP_077520091
VERSION     XP_077520091.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077663965.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 24% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..250
                     /product="uncharacterized protein LOC144129903"
                     /calculated_mol_wt=27179
     Region          120..>232
                     /region_name="YqaJ"
                     /note="YqaJ-like viral recombinase domain; cl09232"
                     /db_xref="CDD:447697"
     Region          212..>250
                     /region_name="PDDEXK_nuclease-like"
                     /note="PDDEXK family nucleases; cl40440"
                     /db_xref="CDD:477358"
     CDS             1..250
                     /gene="LOC144129903"
                     /coded_by="XM_077663965.1:1..753"
                     /db_xref="GeneID:144129903"
ORIGIN      
        1 mvlvdiglvr vnnwtcqqle yamckrevih mlkdgadnvy dvfygahllg eccthvaafl
       61 frveataqyg lndpsptdva ckwieaskgs taapvseief fkpkigrapp qvseapdysv
      121 pvltdeqvsr flhqvkeiap ntliltsisd sedtdsapet aalepvrvgr ckqlsakppl
      181 aeiyqdldid egkkveltaa ccseieeatr gqarsprwlq errgritasv mhrvaacstg
      241 aaglvaeimg
//
LOCUS       XP_077520136             120 aa            linear   INV 25-NOV-2025
DEFINITION  peroxynitrite isomerase THAP4-like [Amblyomma americanum].
ACCESSION   XP_077520136
VERSION     XP_077520136.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664010.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 20% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..120
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..120
                     /product="peroxynitrite isomerase THAP4-like"
                     /calculated_mol_wt=13732
     Region          4..79
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..120
                     /gene="LOC144129953"
                     /coded_by="XM_077664010.1:174..536"
                     /db_xref="GeneID:144129953"
ORIGIN      
        1 mpgccvpqcs nhsrngwrmf hfprdqkrrl lwlvrikrdk wqptnsscvc gahledksfe
       61 qnradgwnkl kpnavptvfp lrelakerrp prdrsvhvpa lfsddaaaqg sspevdklrt
//
LOCUS       XP_077520161             193 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129983 [Amblyomma americanum].
ACCESSION   XP_077520161
VERSION     XP_077520161.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664035.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..193
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..193
                     /product="uncharacterized protein LOC144129983"
                     /calculated_mol_wt=20515
     CDS             1..193
                     /gene="LOC144129983"
                     /coded_by="XM_077664035.1:1..582"
                     /db_xref="GeneID:144129983"
ORIGIN      
        1 mavlastarg rksgrqdgrl qcacvtfqni anssqdasir kliavlaagt taegpqqett
       61 saaagpgyda aasceaasas eppflstgfv aicplgihhc apatstssqs eriklgiecp
      121 lvsgrggtmm ksindlhtff vlqlplhncm dmvahfhaqi kylqlhwslw kqvlhsgqls
      181 mypagfkhfw dsi
//
LOCUS       XP_077520172             312 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144129994 [Amblyomma americanum].
ACCESSION   XP_077520172
VERSION     XP_077520172.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664046.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 47% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..312
                     /product="uncharacterized protein LOC144129994"
                     /calculated_mol_wt=35405
     CDS             1..312
                     /gene="LOC144129994"
                     /coded_by="XM_077664046.1:1..939"
                     /db_xref="GeneID:144129994"
ORIGIN      
        1 mchnldlsnp avlspliavc icrartaswh credicpqdv qalcgwlrps tghtkrvcki
       61 lrsewmrqar flkdvlwsrc asgtplqnqr vfrywcrttd qlteflwnvv rsslpkakrr
      121 syaggkllvl gdiavdprha qtlrlgpkfc lesgmmtver lelardvsyk apaadrtkce
      181 sdcidavsrc vsskgkynqf rslvdylydn rlrtvqsdke gffvvmsedq fsekahgavm
      241 knfksvtrnp vlskkravtl lrkfeleerl lhtsgevpme giqdehawtp qecarspipd
      301 didpthqgas si
//
LOCUS       XP_077520197             177 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130030 [Amblyomma americanum].
ACCESSION   XP_077520197
VERSION     XP_077520197.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664071.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 34% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..177
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..177
                     /product="uncharacterized protein LOC144130030"
                     /calculated_mol_wt=19235
     Region          62..82
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(67,69,71,73..74,77..78,81,95,97,101..102,105..106,
                     109,123,125,127,129..130,133..134,137)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          70..>139
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          90..110
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          118..138
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          146..162
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..177
                     /gene="LOC144130030"
                     /coded_by="XM_077664071.1:1..534"
                     /db_xref="GeneID:144130030"
ORIGIN      
        1 mrpsktgrtp dsgpveggds ardgnmlght lpfgcierna vagrsevkta ttakaaersf
       61 scgqcgkrfk rsstlsthll ihsdtrpypc hfcgkrfhqk sdmkkhtyih tgekphkcvv
      121 cgkafsqssn lithtrkhsg fkpfacrvcg rafqrkvdlr rhadsqhcaa aglsaap
//
LOCUS       XP_077520200             330 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130039 [Amblyomma americanum].
ACCESSION   XP_077520200
VERSION     XP_077520200.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664074.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 18% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..330
                     /product="uncharacterized protein LOC144130039"
                     /calculated_mol_wt=36833
     Region          <241..>284
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     CDS             1..330
                     /gene="LOC144130039"
                     /coded_by="XM_077664074.1:14..1006"
                     /db_xref="GeneID:144130039"
ORIGIN      
        1 msisgleppp pflpvpgrpa vawpqwfrif enyvlasgas dctpdrrkal llhslgvegq
       61 rifytlplpp ldnvklgeqt atektsedsk gtgdtrpevs sydiavaalh ahfsatsnvv
      121 aerhrfsrrv qqagesvney italrelsvs csfpaeedsl rdqfvagiss rslrerllle
      181 gsslsfakav llarqfelay ndlqefpsvd vgrintrgnt ptrtpesdes skwshnrqyr
      241 cyrcgssrhn aasvrcpaks krchhcgvmg hfrsvcqkrs sapvlasrkv lcitskrdpr
      301 csqcnlnfga frsrydhwsr kssigwkiwt
//
LOCUS       XP_077520207             350 aa            linear   INV 25-NOV-2025
DEFINITION  dorsal-ventral patterning tolloid-like protein 1 [Amblyomma
            americanum].
ACCESSION   XP_077520207
VERSION     XP_077520207.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664081.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 23% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..350
                     /product="dorsal-ventral patterning tolloid-like protein
                     1"
                     /calculated_mol_wt=39233
     Region          85..194
                     /region_name="CUB"
                     /note="CUB domain; pfam00431"
                     /db_xref="CDD:395345"
     Site            order(95,97,124,129,167,191,193,195..196)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     Region          198..313
                     /region_name="CUB"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; cd00041"
                     /db_xref="CDD:238001"
     Site            order(207,209,214,241,246,284,308,310,312..313)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     CDS             1..350
                     /gene="LOC144130049"
                     /coded_by="XM_077664081.1:1..1053"
                     /db_xref="GeneID:144130049"
ORIGIN      
        1 mdangelwvr itvdcallst rladritqvh rdederfspr sdhnrlllsf fhlastwrac
       61 pgpmrvpekv lrscgsavca ergtcthqis aplgeinspn ypdaypsrkd cawlftttpg
      121 hrlklifndf elephqecay dhislydgds tdaptlgrfc gakvphpils ttnrmymvfk
      181 sdasvqrkgf kashstacgg rllatgrtve hlyshakygd qnyenredcd wlveaesgrv
      241 rlrfltfele heqdcgydfv qlfdgyddsa pqlgrfcgnk mpgeftsssg slllrfrsdd
      301 ninakgfsaa ysavdaaggd lsqqhaptpp aasfaethhr drrlrlhrrh
//
LOCUS       XP_077520212             449 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130059 [Amblyomma americanum].
ACCESSION   XP_077520212
VERSION     XP_077520212.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664086.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..449
                     /product="uncharacterized protein LOC144130059"
                     /calculated_mol_wt=51309
     Region          108..196
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     Region          <364..>401
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     CDS             1..449
                     /gene="LOC144130059"
                     /coded_by="XM_077664086.1:1..1350"
                     /db_xref="GeneID:144130059"
ORIGIN      
        1 myrtpptsrd pspdrkentn vvpdnrasrr lqqlppehgl lpkknkkiva eatpmaapas
       61 pivlqqprep ptfrgstfed peswletyer vatfnswasd dklrhvyfal edaartwfen
      121 reatlttwdl frssflqtft svlrkeraqv lletraqlpn etiailteem srvfrhadpe
      181 mskekkvrll mrgvkeelfg amtrsppttv eeflreatsi ektlemrnrq ynrrtnstny
      241 agaqslatdd lrktiiavvr eelqrifpss qpqvasvaei vkeevqrplg vrevppqssq
      301 pqpeamayaa varrqgpppr prqdpvtpqf rrpppppptr ppvdqrsypr ktdvwrapdh
      361 rplcyhcgea ghvyrrcpyr dpglrgfavd aprprggerp rdiadylaat qwsprrpsrs
      421 pspgryvspq rrpytgptrg rsaspypen
//
LOCUS       XP_077520219              96 aa            linear   INV 25-NOV-2025
DEFINITION  importin-7-like [Amblyomma americanum].
ACCESSION   XP_077520219
VERSION     XP_077520219.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664093.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..96
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..96
                     /product="importin-7-like"
                     /calculated_mol_wt=10526
     CDS             1..96
                     /gene="LOC144130068"
                     /coded_by="XM_077664093.1:85..375"
                     /db_xref="GeneID:144130068"
ORIGIN      
        1 mesttmslhe eqcpvdeypv akevmraiqn tdpmcfgalt sgpqfveqkr sqqeicvltd
       61 qtkaaaesnr ieksggyvfq nqtlpppfss ggkpfr
//
LOCUS       XP_077520229             303 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130077 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077520229
VERSION     XP_077520229.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664103.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..303
                     /product="uncharacterized protein LOC144130077 isoform X1"
                     /calculated_mol_wt=33683
     Region          5..>66
                     /region_name="THAP"
                     /note="The THAP domain is a putative DNA-binding domain
                     (DBD) and probably also binds a zinc ion; smart00980"
                     /db_xref="CDD:214951"
     CDS             1..303
                     /gene="LOC144130077"
                     /coded_by="XM_077664103.1:39..950"
                     /db_xref="GeneID:144130077"
ORIGIN      
        1 mpttcvaygc naqyekgskl gffrfpnasr dprrreawik avrrlddhgr pwapssgsrl
       61 cgnhfetgtd evlclnacav yhelfcswtg rprmsprhpd fvpslfsfsc kkakrasavi
      121 rfqramerat kkklreqgsi psgtlasavq gggdddmdid gdsegdlhgt qsqqssdyaa
      181 agassnrsqn ecdavhglll lqtaatetsd indiqaseer cpqssatllq enlalkkevq
      241 kwkvkykkle rrtlsvdnid ptsfkfytgl anrglfdaly nhvlnnanim qywdcaekkk
      301 grk
//
LOCUS       XP_077520235             281 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130077 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077520235
VERSION     XP_077520235.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664109.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..281
                     /product="uncharacterized protein LOC144130077 isoform X2"
                     /calculated_mol_wt=31226
     Region          5..85
                     /region_name="THAP"
                     /note="The THAP domain is a putative DNA-binding domain
                     (DBD) and probably also binds a zinc ion; smart00980"
                     /db_xref="CDD:214951"
     CDS             1..281
                     /gene="LOC144130077"
                     /coded_by="XM_077664109.1:39..884"
                     /db_xref="GeneID:144130077"
ORIGIN      
        1 mpttcvaygc naqyekgskl gffrfpnasr dprrreawik avrrlddhgr pwapssgsrl
       61 cgnhfetgrp rmsprhpdfv pslfsfsckk akrasavirf qrameratkk klreqgsips
      121 gtlasavqgg gdddmdidgd segdlhgtqs qqssdyaaag assnrsqnec davhgllllq
      181 taatetsdin diqaseercp qssatllqen lalkkevqkw kvkykklerr tlsvdnidpt
      241 sfkfytglan rglfdalynh vlnnanimqy wdcaekkkgr k
//
LOCUS       XP_077520269             142 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130128 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077520269
VERSION     XP_077520269.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664143.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..142
                     /product="uncharacterized protein LOC144130128 isoform X1"
                     /calculated_mol_wt=14799
     Region          64..>95
                     /region_name="DM4_12"
                     /note="DM4/DM12 family; cl11657"
                     /db_xref="CDD:472236"
     CDS             1..142
                     /gene="LOC144130128"
                     /coded_by="XM_077664143.1:146..574"
                     /db_xref="GeneID:144130128"
ORIGIN      
        1 marsllllll lllavargdl sdlvssvtls ptfkaklasa gpvtatygvs fplelelsgp
       61 tadrrallra aqeameragl dgaacvrraq ceaaaggrrq gllgelldll lrgprcdgan
      121 esvdgvncsh efrscpyslf ka
//
LOCUS       XP_077520275             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130128 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077520275
VERSION     XP_077520275.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664149.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144130128 isoform X2"
                     /calculated_mol_wt=13968
     Region          64..>95
                     /region_name="DM4_12"
                     /note="DM4/DM12 family; cl11657"
                     /db_xref="CDD:472236"
     CDS             1..133
                     /gene="LOC144130128"
                     /coded_by="XM_077664149.1:152..553"
                     /db_xref="GeneID:144130128"
ORIGIN      
        1 marsllllll lllavargdl sdlvssvtls ptfkaklasa gpvtatygvs fplelelsgp
       61 tadrrallra aqeameragl dgaacvrraq ceaaaggrrq gllgelldll lrclpslcnc
      121 fsplyhlppv hqf
//
LOCUS       XP_077520285             628 aa            linear   INV 25-NOV-2025
DEFINITION  cubilin-like [Amblyomma americanum].
ACCESSION   XP_077520285
VERSION     XP_077520285.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664159.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..628
                     /product="cubilin-like"
                     /calculated_mol_wt=70748
     Region          27..136
                     /region_name="CUB"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; cd00041"
                     /db_xref="CDD:238001"
     Site            order(35,37,39,66,71,107,131,133,135..136)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     Region          255..347
                     /region_name="CUB"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; cd00041"
                     /db_xref="CDD:238001"
     Region          367..479
                     /region_name="CUB"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; cd00041"
                     /db_xref="CDD:238001"
     Site            order(375,377,379,406,411,450,474,476,478..479)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     Region          481..575
                     /region_name="CUB"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; cd00041"
                     /db_xref="CDD:238001"
     CDS             1..628
                     /gene="LOC144130144"
                     /coded_by="XM_077664159.1:29..1915"
                     /db_xref="GeneID:144130144"
ORIGIN      
        1 mppaqvrsll vlllvlwpaa warnascggf ltqfrghfqs pnfpdhydds avcrwyifap
       61 kefailiekh aidieddasr sdcpydflry sfgnwkktfc gkgvvepill dtnfvtvvmk
      121 tdasmsgmgf nisyrfvlpp cvrvftsseg llevpedkrh tlirrrrwcf rsargnsyqf
      181 tfdyfdlpge ppkcrhmymk vleksegqvr shprlcgkls pepvhtrsss vsllflsdra
      241 ldrsglhvsw rvlgcneehr aasgrlrfpr dgvyaafpat crwtvyagsd qtveltltrl
      301 dlqrcdleyl rifegedegr tllddlcrld apmvitstrr ilslqlrlys lrrdaveasy
      361 qmkatpcggl yeasegsivs padlaqadsg ltclwtirvk nnylprvefr eftfgesenc
      421 sagyieayfl sqgsqmqlmp rrcsaepppp vvgidnevri klhverrdpr thfrlrfrsv
      481 cetvvianrt ggvsqsfgfp klyehesecs wnvyapkger lalfvtelrf skskycttsa
      541 ltvtdgtqpt spvvavlsra esraflssed alalricggv vkfvymsvpa adtcskaeps
      601 teptkrevag kprvgaavvw eeidqtps
//
LOCUS       XP_077520290             253 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130154 [Amblyomma americanum].
ACCESSION   XP_077520290
VERSION     XP_077520290.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664164.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..253
                     /product="uncharacterized protein LOC144130154"
                     /calculated_mol_wt=27168
     CDS             1..253
                     /gene="LOC144130154"
                     /coded_by="XM_077664164.1:12..773"
                     /db_xref="GeneID:144130154"
ORIGIN      
        1 melkpaalll lllllspgtr pqemrvesgq alaacrdthg rlpsgalsyd ytkallarle
       61 acvvfnrrfm tenlpielse lrvspkiqvp psselkrlte eqaflriyql lvayatfaqh
      121 vantthgvas csghatskrs sagaatalqa aansqrnadq lqelrcdvrl ssaahgsqaq
      181 faaadasaag llldtyadcs krllahysva lgvqqllhsl qgyllhlvrl kspypgaena
      241 eapavevfeq aty
//
LOCUS       XP_077520297             242 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130163 [Amblyomma americanum].
ACCESSION   XP_077520297
VERSION     XP_077520297.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664171.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..242
                     /product="uncharacterized protein LOC144130163"
                     /calculated_mol_wt=26145
     CDS             1..242
                     /gene="LOC144130163"
                     /coded_by="XM_077664171.1:103..831"
                     /db_xref="GeneID:144130163"
ORIGIN      
        1 mvqplrragp plllgglvll vmsesvasmp aaewrrisde ayfsdllypc sddlpqvqpa
       61 gpptdtfaie yvkilgertt aclghatkll avnlpescss lqdvmeapdq dtlqslsqaq
      121 altnlygflv shaayaffvs eqaekyanss caipnehsla ensrtlaaql rdllctiklt
      181 asvhggnvef saseervaal rvcdestcsr rllrdcqvva svtnvlhtlq rylmglqpde
      241 as
//
LOCUS       XP_077520305             136 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130179 [Amblyomma americanum].
ACCESSION   XP_077520305
VERSION     XP_077520305.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664179.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..136
                     /product="uncharacterized protein LOC144130179"
                     /calculated_mol_wt=14962
     CDS             1..136
                     /gene="LOC144130179"
                     /coded_by="XM_077664179.1:461..871"
                     /db_xref="GeneID:144130179"
ORIGIN      
        1 msehaeviqm hhekygphmr nfvrdntvga cvwvfalsla vlvilrmlkm iwgdiaavvh
       61 lykrytaerr kgvtrnvafh atalallaad tsrmealpvv grshttsera edqaqeptss
      121 akdhvtagmq alptsl
//
LOCUS       XP_077520319             176 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130209 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077520319
VERSION     XP_077520319.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664193.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..176
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..176
                     /product="uncharacterized protein LOC144130209 isoform X1"
                     /calculated_mol_wt=18872
     CDS             1..176
                     /gene="LOC144130209"
                     /coded_by="XM_077664193.1:375..905"
                     /db_xref="GeneID:144130209"
ORIGIN      
        1 mkpyhvdsrn tsmdvpvmtd mcqggsshvs tpcmevgvte slgalqtvfd gnyedcdegr
       61 glvpkavifc pqtrqeglsg adskkevalw alqkrccmvr dersnrtegc sgnmvrlggk
      121 mslqfdgavt ptvlastiaf vglplvvflg gmmrhrqsal plftdlkvpa livves
//
LOCUS       XP_077520325             176 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130209 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077520325
VERSION     XP_077520325.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664199.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..176
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..176
                     /product="uncharacterized protein LOC144130209 isoform X1"
                     /calculated_mol_wt=18872
     CDS             1..176
                     /gene="LOC144130209"
                     /coded_by="XM_077664199.1:252..782"
                     /db_xref="GeneID:144130209"
ORIGIN      
        1 mkpyhvdsrn tsmdvpvmtd mcqggsshvs tpcmevgvte slgalqtvfd gnyedcdegr
       61 glvpkavifc pqtrqeglsg adskkevalw alqkrccmvr dersnrtegc sgnmvrlggk
      121 mslqfdgavt ptvlastiaf vglplvvflg gmmrhrqsal plftdlkvpa livves
//
LOCUS       XP_077520326              99 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130209 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077520326
VERSION     XP_077520326.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664200.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..99
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..99
                     /product="uncharacterized protein LOC144130209 isoform X2"
                     /calculated_mol_wt=11508
     CDS             1..99
                     /gene="LOC144130209"
                     /coded_by="XM_077664200.1:1071..1370"
                     /db_xref="GeneID:144130209"
ORIGIN      
        1 mmrdrfamav flyaydgysk ylvclprtls ylygsetctl ptwcrkpsgr skedifeaca
       61 ylydmlamse haeviqmhhe kyephmrnfv rdntlgacv
//
LOCUS       XP_077520332              99 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130209 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077520332
VERSION     XP_077520332.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664206.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..99
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..99
                     /product="uncharacterized protein LOC144130209 isoform X2"
                     /calculated_mol_wt=11508
     CDS             1..99
                     /gene="LOC144130209"
                     /coded_by="XM_077664206.1:725..1024"
                     /db_xref="GeneID:144130209"
ORIGIN      
        1 mmrdrfamav flyaydgysk ylvclprtls ylygsetctl ptwcrkpsgr skedifeaca
       61 ylydmlamse haeviqmhhe kyephmrnfv rdntlgacv
//
LOCUS       XP_077520336              99 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130209 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077520336
VERSION     XP_077520336.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664210.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..99
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..99
                     /product="uncharacterized protein LOC144130209 isoform X2"
                     /calculated_mol_wt=11508
     CDS             1..99
                     /gene="LOC144130209"
                     /coded_by="XM_077664210.1:602..901"
                     /db_xref="GeneID:144130209"
ORIGIN      
        1 mmrdrfamav flyaydgysk ylvclprtls ylygsetctl ptwcrkpsgr skedifeaca
       61 ylydmlamse haeviqmhhe kyephmrnfv rdntlgacv
//
LOCUS       XP_077520340             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130240 [Amblyomma americanum].
ACCESSION   XP_077520340
VERSION     XP_077520340.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664214.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144130240"
                     /calculated_mol_wt=16955
     CDS             1..158
                     /gene="LOC144130240"
                     /coded_by="XM_077664214.1:244..720"
                     /db_xref="GeneID:144130240"
ORIGIN      
        1 mepllagkeq rckvisilgp itasravsah nvghnqkcpd vnpavapevv dlhgapessd
       61 alhpsldnas qshvlvevrq tlkpqgapqh crrftgfdan iflndaedpf msrhligrac
      121 vvsivspvtk rdalatrlal rlvmfritts aasrplql
//
LOCUS       XP_077520342             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130251 [Amblyomma americanum].
ACCESSION   XP_077520342
VERSION     XP_077520342.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664216.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144130251"
                     /calculated_mol_wt=13983
     CDS             1..127
                     /gene="LOC144130251"
                     /coded_by="XM_077664216.1:936..1319"
                     /db_xref="GeneID:144130251"
ORIGIN      
        1 mhhekygphm wnfvrdntvg acvwvfalsl avlvilrmlk miwgdiaavv hlykrytaer
       61 rkgvtrndaf hatalallaa dtsrmealpv vgrshttser aedqaqepts sakdhvtagm
      121 qalptsl
//
LOCUS       XP_077520350             161 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130259 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077520350
VERSION     XP_077520350.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664224.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..161
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..161
                     /product="uncharacterized protein LOC144130259 isoform X1"
                     /calculated_mol_wt=18647
     Region          <51..136
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          88..108
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(93,95,97,99..100,103..104,107,121,123,127..128,
                     131..132,135,149,151,153,155..156)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          116..136
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          128..150
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     CDS             1..161
                     /gene="LOC144130259"
                     /coded_by="XM_077664224.1:160..645"
                     /db_xref="GeneID:144130259"
ORIGIN      
        1 mmpgtlsrlh gsrrtcansc sikidakelm rkrttaglfl sakdvdivpl nslffpcekc
       61 gdlfstqynl emhtrrkhwh sthgirrcty ctytssrkfn iirherthvg rreslcplcq
      121 ksfsqpknlk vhmnihsgek pyecgqcgkr lrqrvharrl e
//
LOCUS       XP_077520357             132 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130259 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077520357
VERSION     XP_077520357.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664231.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..132
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..132
                     /product="uncharacterized protein LOC144130259 isoform X2"
                     /calculated_mol_wt=15488
     Region          <22..107
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          59..79
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(64,66,68,70..71,74..75,78,92,94,98..99,102..103,106,
                     120,122,124,126..127)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          87..107
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          99..121
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     CDS             1..132
                     /gene="LOC144130259"
                     /coded_by="XM_077664231.1:363..761"
                     /db_xref="GeneID:144130259"
ORIGIN      
        1 mrkrttaglf lsakdvdivp lnslffpcek cgdlfstqyn lemhtrrkhw hsthgirrct
       61 yctytssrkf niirherthv grreslcplc qksfsqpknl kvhmnihsge kpyecgqcgk
      121 rlrqrvharr le
//
LOCUS       XP_077520380             136 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130302 [Amblyomma americanum].
ACCESSION   XP_077520380
VERSION     XP_077520380.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664254.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..136
                     /product="uncharacterized protein LOC144130302"
                     /calculated_mol_wt=14874
     CDS             1..136
                     /gene="LOC144130302"
                     /coded_by="XM_077664254.1:972..1382"
                     /db_xref="GeneID:144130302"
ORIGIN      
        1 msehaeviqm hhekygphmr nfvrdntvga cvwvcalsla vlvilrmlkm iwgdiaavvh
       61 lykrytaerr kgvtrngafh avalallaad tsrmealpvv grshttsera edqaqeptss
      121 akdhvtagmq alptsl
//
LOCUS       XP_077520399             205 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130339 [Amblyomma americanum].
ACCESSION   XP_077520399
VERSION     XP_077520399.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664273.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..205
                     /product="uncharacterized protein LOC144130339"
                     /calculated_mol_wt=22164
     CDS             1..205
                     /gene="LOC144130339"
                     /coded_by="XM_077664273.1:164..781"
                     /db_xref="GeneID:144130339"
ORIGIN      
        1 mlkmiwgdia avvhlykryt aerrkgvtqn gafhavalal latdtspmep llagkeqrck
       61 visilgpita sravsahnvg hnqkcpdvnp avapevvdlh gapessdalh psldnasqsh
      121 vlvevrqtlk pqgapqhcrr ftgfdanifl ndaedpfmsr hligracvvr ivspvtkraa
      181 latrlalrlv mfrittssas rplql
//
LOCUS       XP_077520408             295 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130349 [Amblyomma americanum].
ACCESSION   XP_077520408
VERSION     XP_077520408.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664282.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..295
                     /product="uncharacterized protein LOC144130349"
                     /calculated_mol_wt=31409
     CDS             1..295
                     /gene="LOC144130349"
                     /coded_by="XM_077664282.1:78..965"
                     /db_xref="GeneID:144130349"
ORIGIN      
        1 mkpyhldsrn tsmdvpvmtd mcqggsshvs tpcmevgvte slgalqtvfd gnyedcdegr
       61 glvpkavifc pltrqeglsg adskkevalw alqkrccmvr dersnrtegc sgnmvrlggn
      121 vslqfdgavt ptvlasaiaf vglplvvflg gmmrhltahf aavhrpegas adcdrvlnlr
      181 rprgctales rgatqgeaqc ilcrlspdqa alhgvagrlv qgewspaglg vsddarpfcy
      241 ggvlvlvrrl qqvprvpaph avvpirlgdl ytshvvpeav gsvegrhlrg vrisl
//
LOCUS       XP_077520417             205 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130361 [Amblyomma americanum].
ACCESSION   XP_077520417
VERSION     XP_077520417.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664291.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..205
                     /product="uncharacterized protein LOC144130361"
                     /calculated_mol_wt=22126
     CDS             1..205
                     /gene="LOC144130361"
                     /coded_by="XM_077664291.1:53..670"
                     /db_xref="GeneID:144130361"
ORIGIN      
        1 mlkmiwgdia aavhlykryt aerrkgvtrn gafhavalal latdtspmea llagkeqrck
       61 visilgpita sravsahnvg hnqkcpdvnp avapevvdlh gapessdalh psldnasqsh
      121 vlvevrqtlk pqgapqhcrr ftgfdanifl sdaedpfmsr hqigracvvr ivspvtkraa
      181 latrlalrlv mfrittssas rplql
//
LOCUS       XP_077520432             205 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130380 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077520432
VERSION     XP_077520432.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664306.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..205
                     /product="uncharacterized protein LOC144130380 isoform X1"
                     /calculated_mol_wt=22195
     CDS             1..205
                     /gene="LOC144130380"
                     /coded_by="XM_077664306.1:333..950"
                     /db_xref="GeneID:144130380"
ORIGIN      
        1 mlkmiwgdia avvhlykryt aerrkgvtrn gafhavalal latdtspmea llagkvqrck
       61 visilgpita sravsahnvg hnqkcpdvnp avapevvdlh gapessdalh psldnasqsh
      121 vlvevrqtlk pqgvpqhcrr ftgfdanifl ndaedpfmsr hqigrscvvr ivspvtkraa
      181 latrlalrlv mfrittssas rplql
//
LOCUS       XP_077520438              92 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130380 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077520438
VERSION     XP_077520438.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664312.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..92
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..92
                     /product="uncharacterized protein LOC144130380 isoform X2"
                     /calculated_mol_wt=10572
     CDS             1..92
                     /gene="LOC144130380"
                     /coded_by="XM_077664312.1:15..293"
                     /db_xref="GeneID:144130380"
ORIGIN      
        1 maaflyaydg yskylvclpr tlsylygset ctlptwcrkp sgrskedife acaylydmla
       61 msehaeviqm hhekyephmr nfvrdntlga cv
//
LOCUS       XP_077520450             205 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130395 [Amblyomma americanum].
ACCESSION   XP_077520450
VERSION     XP_077520450.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664324.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..205
                     /product="uncharacterized protein LOC144130395"
                     /calculated_mol_wt=22302
     CDS             1..205
                     /gene="LOC144130395"
                     /coded_by="XM_077664324.1:252..869"
                     /db_xref="GeneID:144130395"
ORIGIN      
        1 mlkmiwgdia avvhlykryt aerrkgvtqn gafhavalal latdtspmep llagkeqrck
       61 visilgpita sravsahnvv hnqkcpkvnp avalevvdlh gapessdalh psldnasqsh
      121 vlvefrqtlk pqgapqhcrr ftgfdanifl ndaedpfmsr hqiaracvvk ivspvtkraa
      181 latrlalrlv mfrmttssas rplql
//
LOCUS       XP_077520471             520 aa            linear   INV 25-NOV-2025
DEFINITION  EBF transcription factor knot isoform X1 [Amblyomma americanum].
ACCESSION   XP_077520471
VERSION     XP_077520471.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664345.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..520
                     /product="EBF transcription factor knot isoform X1"
                     /calculated_mol_wt=56462
     Region          58..259
                     /region_name="COE_DBD"
                     /note="Colier/Olf/Early B-cell factor (EBF) DNA Binding
                     Domain; cd11606"
                     /db_xref="CDD:212156"
     Site            order(75..79,169,172..175,181..184,186,209,211,213..217,
                     245,247..252)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:212156"
     Region          270..354
                     /region_name="IPT_COE"
                     /note="IPT domain of the COE family (Col/Olf-1/EBF) of
                     non-basic, helix-loop-helix (HLH)-containing transcription
                     factors. COE family proteins are all transcription factors
                     and play an important role in variety of developmental
                     processes. Mouse EBF is involved...; cd01175"
                     /db_xref="CDD:238580"
     Region          355..398
                     /region_name="COE1_HLH"
                     /note="Transcription factor COE1 helix-loop-helix domain;
                     pfam16423"
                     /db_xref="CDD:406751"
     CDS             1..520
                     /gene="kn"
                     /coded_by="XM_077664345.1:115..1677"
                     /db_xref="GeneID:144130419"
ORIGIN      
        1 mfglqdaglr gpmaglkeep laplraagwm qpamldqtrn meavcseylv psqrmlfsvg
       61 lgrahfekqp psnlrksnff hfvialydka gqpveverta fvgfiekgqe sdgqktnngi
      121 hyrlqllyan gvrqeqdlyv rlidsvtkqa ivyegqdknp emcrvllthe vmcsrccdkk
      181 scgnrnetps dpviidrffl kfflkcnqnc lknagnprdm rrfqvvisnq vsvegpllav
      241 sdnmfvhnns khgrrakrld ptevpfaatp cikaispseg wttggstvii vgdnffdglq
      301 vvfgtmlvws elitshairv qtpprhipgv vevtlsyksk qfckgapgrf vyvslnepti
      361 dygfqrlakl iprhpgdpek lpkeiilkra adlaealysm prqnqlslqg prspalsggs
      421 tgfnayaaag qlavnvqene ygrsqsssvs prgsnastph sssgaaggpy aagplpgspg
      481 llngmggivp shfaamnpfa lqptcngqsy ggsivtsgak
//
LOCUS       XP_077520477             476 aa            linear   INV 25-NOV-2025
DEFINITION  EBF transcription factor knot isoform X2 [Amblyomma americanum].
ACCESSION   XP_077520477
VERSION     XP_077520477.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664351.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..476
                     /product="EBF transcription factor knot isoform X2"
                     /calculated_mol_wt=52308
     Region          58..259
                     /region_name="COE_DBD"
                     /note="Colier/Olf/Early B-cell factor (EBF) DNA Binding
                     Domain; cd11606"
                     /db_xref="CDD:212156"
     Site            order(75..79,169,172..175,181..184,186,209,211,213..217,
                     245,247..252)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:212156"
     Region          270..354
                     /region_name="IPT_COE"
                     /note="IPT domain of the COE family (Col/Olf-1/EBF) of
                     non-basic, helix-loop-helix (HLH)-containing transcription
                     factors. COE family proteins are all transcription factors
                     and play an important role in variety of developmental
                     processes. Mouse EBF is involved...; cd01175"
                     /db_xref="CDD:238580"
     Region          355..398
                     /region_name="COE1_HLH"
                     /note="Transcription factor COE1 helix-loop-helix domain;
                     pfam16423"
                     /db_xref="CDD:406751"
     CDS             1..476
                     /gene="kn"
                     /coded_by="XM_077664351.1:115..1545"
                     /db_xref="GeneID:144130419"
ORIGIN      
        1 mfglqdaglr gpmaglkeep laplraagwm qpamldqtrn meavcseylv psqrmlfsvg
       61 lgrahfekqp psnlrksnff hfvialydka gqpveverta fvgfiekgqe sdgqktnngi
      121 hyrlqllyan gvrqeqdlyv rlidsvtkqa ivyegqdknp emcrvllthe vmcsrccdkk
      181 scgnrnetps dpviidrffl kfflkcnqnc lknagnprdm rrfqvvisnq vsvegpllav
      241 sdnmfvhnns khgrrakrld ptevpfaatp cikaispseg wttggstvii vgdnffdglq
      301 vvfgtmlvws elitshairv qtpprhipgv vevtlsyksk qfckgapgrf vyvslnepti
      361 dygfqrlakl iprhpgdpek lpkeiilkra adlaealysm prqnqlslqg prspalsggs
      421 tgfnayaaag qlavnvqeng mggivpshfa amnpfalqpt cngqsyggsi vtsgak
//
LOCUS       XP_077520495             902 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130447 [Amblyomma americanum].
ACCESSION   XP_077520495
VERSION     XP_077520495.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664369.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..902
                     /product="uncharacterized protein LOC144130447"
                     /calculated_mol_wt=101560
     Region          4..79
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          213..430
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..902
                     /gene="LOC144130447"
                     /coded_by="XM_077664369.1:128..2836"
                     /db_xref="GeneID:144130447"
ORIGIN      
        1 mpgccvpqct nhsrkgwrmf qfprdpkrrl lwtvktkrdk wqptntscic sahfepssfe
       61 qhrsdgwkkl kpnavptlfs fkplpkerka pkeraaseis lsngksdatp veassssceg
      121 itlsatteqs qfrpplqedt semdsfqvsn viaecpqqvq dadrreqage psgtqtassy
      181 saelkkrlad itrkytdlqd hhaaarnsik glknqvqrle seirnfsknv kflnedqlra
      241 lsrsnnrgns wtpetvkqgl qikfasgttg yetlrklgyp lpsnrtltrr leglkfllgi
      301 lhevidvmrc kamameevek dcilfldeme issgyeldra edallggktl ppkpdepanh
      361 alvfmlsgln qrwkqviase itghhvegsv lkhyvlelvq lcsqvslrvr vvtsdmgasn
      421 ramwrdfgfs shrnsstvcs iphpcldgkq lfftadpahv lknlrgqlls ssvftlsdat
      481 vkennlpsse vklehvqavl dydaehelkv apnlcevhts sghftkmkvg vavqffreap
      541 aairfliras vieeeaetta wflelifkwy slmsrnptma lsrrsmakyy aalgtlhtam
      601 etirstkmgs tslwkpcqag lliattvvih lqevllgneg ydffltsrll qdclenlfsv
      661 vrlrkpvpsa ydmkcalrlv svsqflftpr ttsydlddre ylvdllsqak kerteveade
      721 indseilfie elsttecsil hyiggfilkg ilpsvscqqc kdallgcasd ehasltalme
      781 yvsegdnliy psddvmhmlk iyeehfvgin swctgklvam kspvrsltay lgkvcckqay
      841 rprlklcash deeirktltd ryarllmill mirllliyfn pavrflvrsm nsfqsstrva
      901 vc
//
LOCUS       XP_077520507             302 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130457 [Amblyomma americanum].
ACCESSION   XP_077520507
VERSION     XP_077520507.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664381.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..302
                     /product="uncharacterized protein LOC144130457"
                     /calculated_mol_wt=33700
     Region          10..99
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..302
                     /gene="LOC144130457"
                     /coded_by="XM_077664381.1:284..1192"
                     /db_xref="GeneID:144130457"
ORIGIN      
        1 msgrrrqrhc fapgcktgyq wtkgeqpslf avpkdesrrk qwernlhrkd kvldetcsvc
       61 elhfepacil rdyihiidgk evriprgkpe llpnavptll pnapsylskk aplqrpprkr
      121 kstsachdea krrsgchsvd deeasctdvr tacsvltndp dtcengetsl pddftrldvp
      181 seywscnrip dysgtvyckl rmkknqaves ervvvlsrdd gpgvaysahl cghlietgri
      241 asceegnqll rkvdsyrlcr galstssvpk ssltkgleaq stvhegtyfs krcagkepse
      301 gm
//
LOCUS       XP_077520543             130 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130504 [Amblyomma americanum].
ACCESSION   XP_077520543
VERSION     XP_077520543.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664417.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..130
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..130
                     /product="uncharacterized protein LOC144130504"
                     /calculated_mol_wt=14637
     CDS             1..130
                     /gene="LOC144130504"
                     /coded_by="XM_077664417.1:10..402"
                     /db_xref="GeneID:144130504"
ORIGIN      
        1 mqhprptrkv khfaskeela dhikkspyge lfsipgtllh ntltttrrei qqpsqapasv
       61 qrqqathhse kvafqspkck erqlnkvepp vddfeitler plcnlnfgkl lgseravdkl
      121 sltmtsakkn
//
LOCUS       XP_077520544             282 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130531 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077520544
VERSION     XP_077520544.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664418.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..282
                     /product="uncharacterized protein LOC144130531 isoform X1"
                     /calculated_mol_wt=31288
     CDS             1..282
                     /gene="LOC144130531"
                     /coded_by="XM_077664418.1:132..980"
                     /db_xref="GeneID:144130531"
ORIGIN      
        1 mqcrffqesn cslhdfqask kkrsnqassk kdnynsilkm qigqahrkns tvthqmrsfv
       61 kkcapllgrn lqrtkmlgcq hdvllsgtvq qrvrrhycrl isylkclili llliftakqp
      121 thkavastst agtstrlpqa sgsatgtske ppqdftylpd gslhlrngiv ltkvqasrii
      181 shkksslvvk etaqaiwtqs glamrsvkgg vaprkrgtae takprltpek vevvaatleh
      241 wgkvnkndit aamknltnil cekiqdcvks errqqltqqa ll
//
LOCUS       XP_077520545             227 aa            linear   INV 25-NOV-2025
DEFINITION  BEN domain-containing protein 5-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077520545
VERSION     XP_077520545.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664419.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..227
                     /product="BEN domain-containing protein 5-like isoform X2"
                     /calculated_mol_wt=24890
     CDS             1..227
                     /gene="LOC144130531"
                     /coded_by="XM_077664419.1:518..1201"
                     /db_xref="GeneID:144130531"
ORIGIN      
        1 mrsfvkkcap llgrnlqrtk mlgcqhdvll sgtvqqrvrr hycrlisylk clililllif
       61 takqpthkav aststagtst rlpqasgsat gtskeppqdf tylpdgslhl rngivltkvq
      121 asriishkks slvvketaqa iwtqsglamr svkggvaprk rgtaetakpr ltpekvevva
      181 atlehwgkvn knditaamkn ltnilcekiq dcvkserrqq ltqqall
//
LOCUS       XP_077520546             211 aa            linear   INV 25-NOV-2025
DEFINITION  BEN domain-containing protein 5-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077520546
VERSION     XP_077520546.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664420.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..211
                     /product="BEN domain-containing protein 5-like isoform X3"
                     /calculated_mol_wt=22478
     CDS             1..211
                     /gene="LOC144130531"
                     /coded_by="XM_077664420.1:701..1336"
                     /db_xref="GeneID:144130531"
ORIGIN      
        1 mdfatgppde vfckevcpat gqksakdena glparcvaer dcsttakqpt hkavaststa
       61 gtstrlpqas gsatgtskep pqdftylpdg slhlrngivl tkvqasriis hkksslvvke
      121 taqaiwtqsg lamrsvkggv aprkrgtaet akprltpekv evvaatlehw gkvnkndita
      181 amknltnilc ekiqdcvkse rrqqltqqal l
//
LOCUS       XP_077520547             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130568 [Amblyomma americanum].
ACCESSION   XP_077520547
VERSION     XP_077520547.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664421.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144130568"
                     /calculated_mol_wt=14086
     CDS             1..127
                     /gene="LOC144130568"
                     /coded_by="XM_077664421.1:642..1025"
                     /db_xref="GeneID:144130568"
ORIGIN      
        1 mehsknqvlh lqvhgdeglh gkeqrtpien kwksldrvyk rtkkdnsssg hhrvtceyek
       61 elaeilekeh sinprlllep gkailpsgcs gtntteasae llpvpvedrl saaaedspas
      121 ktkrrsk
//
LOCUS       XP_077520548             148 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130584 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077520548
VERSION     XP_077520548.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664422.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="uncharacterized protein LOC144130584 isoform X1"
                     /calculated_mol_wt=16807
     CDS             1..148
                     /gene="LOC144130584"
                     /coded_by="XM_077664422.1:424..870"
                     /db_xref="GeneID:144130584"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedrrr rhlnrrltat mkrlsgvhvl
       61 nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar pgevyvvhrc
      121 rrcgakghkt yhcwaycspr rhaaagrg
//
LOCUS       XP_077520549              98 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130584 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077520549
VERSION     XP_077520549.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664423.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..98
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..98
                     /product="uncharacterized protein LOC144130584 isoform X2"
                     /calculated_mol_wt=10652
     CDS             1..98
                     /gene="LOC144130584"
                     /coded_by="XM_077664423.1:503..799"
                     /db_xref="GeneID:144130584"
ORIGIN      
        1 mkrlsgvhvl nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar
       61 pgevyvvhrc rrcgakghkt yhcwaycspr rhaaagrg
//
LOCUS       XP_077520550              98 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130584 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077520550
VERSION     XP_077520550.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664424.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..98
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..98
                     /product="uncharacterized protein LOC144130584 isoform X2"
                     /calculated_mol_wt=10652
     CDS             1..98
                     /gene="LOC144130584"
                     /coded_by="XM_077664424.1:482..778"
                     /db_xref="GeneID:144130584"
ORIGIN      
        1 mkrlsgvhvl nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar
       61 pgevyvvhrc rrcgakghkt yhcwaycspr rhaaagrg
//
LOCUS       XP_077520551             252 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130620 [Amblyomma americanum].
ACCESSION   XP_077520551
VERSION     XP_077520551.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664425.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..252
                     /product="uncharacterized protein LOC144130620"
                     /calculated_mol_wt=28542
     CDS             1..252
                     /gene="LOC144130620"
                     /coded_by="XM_077664425.1:155..913"
                     /db_xref="GeneID:144130620"
ORIGIN      
        1 mvdegqairg gfylrklfei qtqllvkpvr fltrahvepn nlektkvrra vqifsppvia
       61 tlqylqensq chpdasefqd csatitfmrm iakwyslhni asvkpwkqqe esftvsdder
      121 lswleldfvc yvediqiqgg rskrkltket yeatllttrs tvalieylld hvnfryvltq
      181 alnsdpvesl fsslrkfngg ndrvdaraav ftsekllkvg ilqaaktana pmnseakaav
      241 klpiqdgkty af
//
LOCUS       XP_077520552             308 aa            linear   INV 25-NOV-2025
DEFINITION  beta-3 adrenergic receptor-like [Amblyomma americanum].
ACCESSION   XP_077520552
VERSION     XP_077520552.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664426.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..308
                     /product="beta-3 adrenergic receptor-like"
                     /calculated_mol_wt=33840
     Region          2..261
                     /region_name="7tmA_amine_R-like"
                     /note="amine receptors and similar proteins, member of the
                     class A family of seven-transmembrane G protein-coupled
                     receptors; cd14967"
                     /db_xref="CDD:320098"
     Region          5..32
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320098"
     Region          43..73
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320098"
     Site            order(46,50..51,54..55,129,132..133,136,209,212..213,216,
                     232,236,240)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:320098"
     Region          83..103
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320098"
     Region          125..155
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320098"
     Region          189..219
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320098"
     Region          229..254
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320098"
     CDS             1..308
                     /gene="LOC144130652"
                     /coded_by="XM_077664426.1:117..1043"
                     /db_xref="GeneID:144130652"
ORIGIN      
        1 mrsptnvlla slatadvava llvmpprlay dlvrawrlgl lacrlwiscd vmcctasilh
       61 llavaldrfw aitrplryrv srrrlalava aiwlcsaais fvpvllgwhs dgsapdasdc
      121 glrvnavyal vssltsfylp lpvmlyvylr illvagrqar qirllersla gpaspglrhs
      181 lrrrsrqllh dtkavrslgi vlgvfcacwl pfflmylita ccpacdlgyd wscaitwlgy
      241 snsafnpciy aflnrdfraa fgrllgcgsr rrtadaalgp arrhslvrps antgraaiqd
      301 ssegaerr
//
LOCUS       XP_077520553             128 aa            linear   INV 25-NOV-2025
DEFINITION  importin-7-like [Amblyomma americanum].
ACCESSION   XP_077520553
VERSION     XP_077520553.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664427.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..128
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..128
                     /product="importin-7-like"
                     /calculated_mol_wt=14042
     CDS             1..128
                     /gene="LOC144130711"
                     /coded_by="XM_077664427.1:14..400"
                     /db_xref="GeneID:144130711"
ORIGIN      
        1 mtrssrvssv rrewrgapfp ssptsikase qgmestttsl heekcpvdey paskevmrai
       61 qntdpmwfga ltsglqsveq nrtpqeicvl tdqrkaaaes nrieksggyv fqnrtvppsf
      121 ssggtpfr
//
LOCUS       XP_077520554             340 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130728 [Amblyomma americanum].
ACCESSION   XP_077520554
VERSION     XP_077520554.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664428.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..340
                     /product="uncharacterized protein LOC144130728"
                     /calculated_mol_wt=36318
     CDS             1..340
                     /gene="LOC144130728"
                     /coded_by="XM_077664428.1:8..1030"
                     /db_xref="GeneID:144130728"
ORIGIN      
        1 mprtrvvgal lwlaaaaaaa rgelrsrpry phhvasyvpv lvpkhlvlgv gsqelsgery
       61 lrrrppirds teeesqrptd esgepsesve ddraprlvfa rrpaaerrpa tlwiprasrg
      121 adpplstthw rgmdqpdanq sawpsrpagg rirrrraaaa akplattara aasgfipiag
      181 pfggqelays afldggsstp fpagfssyas psrqqhskqa ygggfdygrs ttsafsagyd
      241 slgsgnfqli rggvyaddqa ssshvpyyvq gpsngypays fdedaggpvl gfqgfenfgs
      301 plhnalskhs hvvgastehr ihastlkppa anedqmrate
//
LOCUS       XP_077520556             421 aa            linear   INV 25-NOV-2025
DEFINITION  neuropeptide F receptor-like [Amblyomma americanum].
ACCESSION   XP_077520556
VERSION     XP_077520556.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664430.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..421
                     /product="neuropeptide F receptor-like"
                     /calculated_mol_wt=46891
     Region          72..354
                     /region_name="7tmA_NPYR-like"
                     /note="neuropeptide Y receptors and related proteins,
                     member of the class A family of seven-transmembrane G
                     protein-coupled receptors; cd15203"
                     /db_xref="CDD:320331"
     Region          93..118
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320331"
     Site            order(115,118..119,131..136,138..139,142,184,186..190,225,
                     228..230,232..234,236..237,298,301..302,304..305,308,
                     321..322,324..326,329,332..333)
                     /site_type="other"
                     /note="putative peptide ligand binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:320331"
     Region          131..161
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320331"
     Region          171..192
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320331"
     Region          225..254
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320331"
     Region          278..308
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320331"
     Region          322..347
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320331"
     CDS             1..421
                     /gene="LOC144130798"
                     /coded_by="XM_077664430.1:193..1458"
                     /db_xref="GeneID:144130798"
ORIGIN      
        1 maelgaatrv megvgmspge llallsknrs tnmshdldql esrllsfldr raidgdtlaa
       61 llaaytllil tgaignglvc lavarrpkmr tarnllivnl aaadlllclf tmpfslveia
      121 lkfwplggtt cklvaglqat svfvstlsit aiaidrylvi vyptkqhwcf salvgmpliw
      181 llalllsvpm fvfrvldpid lpdpergtyv hleycvehwp ieegrfyysi fsivfqylmp
      241 ittvtvayas icrrlrvrld akagplnqsa prqqhdkrrm hrtnvllvai aaifaaswlp
      301 lhilnlvads tpsltedsrl yrvyfacchm vgmssacsnp llygwlndnf rrefrrilfl
      361 ccprlrarra satappsesr pltgqalvlf rrqprtedtc rdaqpslrvh ieaseanaei
      421 a
//
LOCUS       XP_077520557             421 aa            linear   INV 25-NOV-2025
DEFINITION  neuropeptide F receptor-like [Amblyomma americanum].
ACCESSION   XP_077520557
VERSION     XP_077520557.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664431.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..421
                     /product="neuropeptide F receptor-like"
                     /calculated_mol_wt=46891
     Region          72..354
                     /region_name="7tmA_NPYR-like"
                     /note="neuropeptide Y receptors and related proteins,
                     member of the class A family of seven-transmembrane G
                     protein-coupled receptors; cd15203"
                     /db_xref="CDD:320331"
     Region          93..118
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320331"
     Site            order(115,118..119,131..136,138..139,142,184,186..190,225,
                     228..230,232..234,236..237,298,301..302,304..305,308,
                     321..322,324..326,329,332..333)
                     /site_type="other"
                     /note="putative peptide ligand binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:320331"
     Region          131..161
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320331"
     Region          171..192
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320331"
     Region          225..254
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320331"
     Region          278..308
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320331"
     Region          322..347
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320331"
     CDS             1..421
                     /gene="LOC144130798"
                     /coded_by="XM_077664431.1:178..1443"
                     /db_xref="GeneID:144130798"
ORIGIN      
        1 maelgaatrv megvgmspge llallsknrs tnmshdldql esrllsfldr raidgdtlaa
       61 llaaytllil tgaignglvc lavarrpkmr tarnllivnl aaadlllclf tmpfslveia
      121 lkfwplggtt cklvaglqat svfvstlsit aiaidrylvi vyptkqhwcf salvgmpliw
      181 llalllsvpm fvfrvldpid lpdpergtyv hleycvehwp ieegrfyysi fsivfqylmp
      241 ittvtvayas icrrlrvrld akagplnqsa prqqhdkrrm hrtnvllvai aaifaaswlp
      301 lhilnlvads tpsltedsrl yrvyfacchm vgmssacsnp llygwlndnf rrefrrilfl
      361 ccprlrarra satappsesr pltgqalvlf rrqprtedtc rdaqpslrvh ieaseanaei
      421 a
//
LOCUS       XP_077520558             174 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130855 [Amblyomma americanum].
ACCESSION   XP_077520558
VERSION     XP_077520558.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664432.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (GKSP01014466.1) annotated
            using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 94 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..174
                     /product="uncharacterized protein LOC144130855"
                     /calculated_mol_wt=18147
     CDS             1..174
                     /gene="LOC144130855"
                     /coded_by="XM_077664432.1:1..525"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 94 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144130855"
ORIGIN      
        1 manlkarlll lvayvvsvav vvsverywns sgyrlsdlln pnnelaaavl lfavigllft
       61 gvvfaaglts lasralashs rpccegrggg shgtarhqes pnsidvgvhp tdttiaaggp
      121 alvaphynvs lmhlaeprpp apscpppdms ypssqsisqt spsikvepky yars
//
LOCUS       XP_077520560             311 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130931 [Amblyomma americanum].
ACCESSION   XP_077520560
VERSION     XP_077520560.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664434.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..311
                     /product="uncharacterized protein LOC144130931"
                     /calculated_mol_wt=34792
     CDS             1..311
                     /gene="LOC144130931"
                     /coded_by="XM_077664434.1:34..969"
                     /db_xref="GeneID:144130931"
ORIGIN      
        1 mlflllhsgd vetnpgpntr sesalenlsf lsepseqmaa ifnllkdvha csvqsaknqa
       61 klaadikaik asqksienki griqerldtl eektdvldqf avkptyvqns lqapstqhds
      121 lqtrlyeled rsrcnnllfr dipdsrkswk etedsvkdtl tgidnlpdls iecthrlcqy
      181 spskcrpiiv kfnnfklkec vlstrsklkd kcitvsedfs patriarikl veftknlpgs
      241 psfqlrynki tvneklytnn astdsivgit mppktdafhg gapkhntait dstvesvsri
      301 spentrterm d
//
LOCUS       XP_077520561             187 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144130947 [Amblyomma americanum].
ACCESSION   XP_077520561
VERSION     XP_077520561.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664435.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="uncharacterized protein LOC144130947"
                     /calculated_mol_wt=20855
     Region          <42..161
                     /region_name="Gag_spuma"
                     /note="Spumavirus gag protein; pfam03276"
                     /db_xref="CDD:460872"
     CDS             1..187
                     /gene="LOC144130947"
                     /coded_by="XM_077664435.1:604..1167"
                     /db_xref="GeneID:144130947"
ORIGIN      
        1 mlgksktali tfdgpfipnm vnwwggkykc hpyrptrqvc yncgqqghrs dvcptldara
       61 crqcgaqntp eghqrqpkcl vcrgdhaags rdckeclkqf kelrgsaygg qqsrqksrsg
      121 qqqrkrwlss kgehsqeqrg sswsrsqnqs rtndspssqg tkggkqqlqq qqqqkkqnpt
      181 ttittag
//
LOCUS       XP_077520562             428 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144131005 [Amblyomma americanum].
ACCESSION   XP_077520562
VERSION     XP_077520562.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664436.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..428
                     /product="uncharacterized protein LOC144131005"
                     /calculated_mol_wt=48315
     Region          57..381
                     /region_name="7tm_classA_rhodopsin-like"
                     /note="rhodopsin receptor-like class A family of the
                     seven-transmembrane G protein-coupled receptor
                     superfamily; cd00637"
                     /db_xref="CDD:410626"
     Region          57..82
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410626"
     Region          89..115
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410626"
     Site            order(111,114..115,127..132,134..135,138,178,180..184,214,
                     218..220,222..224,226..227,345,348..349,351..352,355,
                     365..366,368..370,376,379..380)
                     /site_type="other"
                     /note="putative ligand binding pocket [chemical binding]"
                     /db_xref="CDD:410626"
     Region          127..157
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410626"
     Region          214..240
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410626"
     Region          266..355
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410626"
     CDS             1..428
                     /gene="LOC144131005"
                     /coded_by="XM_077664436.1:240..1526"
                     /db_xref="GeneID:144131005"
ORIGIN      
        1 manasveekl sdqlniqqip dkyigyimyf mlkfqndtse fsqpqlrrtf rssyplflvl
       61 ygslfvssli ancwmvlhin rekrpretvc vylfanalnd ifkllvvmpi slvtlclhnw
      121 vfgsflyfas pivqdfpfyr tlrtfvaiac dryryamnpm krqippllcl lgvclvagil
      181 tlpyaaytkf ldlqkylgqq frgagicain ltenieeyir flfivlyalp valviylhvk
      241 vsveiraqet sqslalqarr esnggeetsv cslassmvpe svhstgscat fpvatrdscp
      301 rseacrgssa afykdssped svdwmkerrt qntvmmmvav yavclvplnv lrliahvvhe
      361 gqehsvhfdl ssafavliaf lptlgtpylf rvwhlstrls vdpcqyrklr rvsgasgggr
      421 klrsnsra
//
LOCUS       XP_077520563             393 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144131037 [Amblyomma americanum].
ACCESSION   XP_077520563
VERSION     XP_077520563.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664437.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..393
                     /product="uncharacterized protein LOC144131037"
                     /calculated_mol_wt=43428
     Region          <167..>254
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     CDS             1..393
                     /gene="LOC144131037"
                     /coded_by="XM_077664437.1:46..1227"
                     /db_xref="GeneID:144131037"
ORIGIN      
        1 mqtinqlfvs lllptltsll sgasfdcapp csidepgryr lplvscpprm taatteeqwl
       61 papkeswmas ssrtstafph ikappppapf cglgdctsaq psnqlifamq vsksytlfak
      121 rssnyilvqf pspqccvaia aecvtvvhsl lllsgdietn pgpndssavl aelqkltagq
      181 tqliaevqgl ksqltttdkt itdlsnrmad lethyqallp lrndiekmqa dtsnvariiq
      241 eletriedae nrsrrnnllf ygipdttdse twaesekpii dvcrnnlqit vepheierah
      301 rlgrhsedkn rpiivkftss ktkesllsng rnlkdtvysv gedfshavrh arkqlvafak
      361 arsakfslrf ktlhiglnry ifdtssntvk qla
//
LOCUS       XP_077520564             407 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-1A adrenergic receptor-like [Amblyomma americanum].
ACCESSION   XP_077520564
VERSION     XP_077520564.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664438.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..407
                     /product="alpha-1A adrenergic receptor-like"
                     /calculated_mol_wt=44253
     Region          51..341
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          70..92
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410628"
     Region          109..131
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410628"
     Region          154..170
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410628"
     Region          191..214
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410628"
     Region          275..297
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410628"
     Region          309..334
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410628"
     CDS             1..407
                     /gene="LOC144131065"
                     /coded_by="XM_077664438.1:409..1632"
                     /db_xref="GeneID:144131065"
ORIGIN      
        1 megngsepwl lsfvenassw tanatepagl apgallkggl llaiillais gnlmvllavf
       61 lnaslrtatn clivnlalad lllgasvlpf saalevldkq wffgqifchv waatdvlcct
      121 asinslcvis vdryigvtrp ltyssvvthr ralvacvlvw llsavisvgp llgwrepppa
      181 dpfvcevtkq tgyvifsvsf sfyiptlvil vlyfriykaa arqtkflrtg vktikgepgq
      241 ltlrvhtpsv agdeaqrvag lqgrlvkfkr qkkaaktlgi vvgvfiicwf pfffllplgt
      301 lcqrctvppl lfdfcfwtgy fnsclnpfiy astsreykra fhailrcqws rpregasflp
      361 pdqawpgnfk vaavpradra gsvaqpplra alrsgscghe gsahavr
//
LOCUS       XP_077520565             136 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144131081 [Amblyomma americanum].
ACCESSION   XP_077520565
VERSION     XP_077520565.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664439.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..136
                     /product="uncharacterized protein LOC144131081"
                     /calculated_mol_wt=14914
     Region          <19..75
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     Region          41..>125
                     /region_name="class_II_aaRS-like_core"
                     /note="Class II tRNA amino-acyl synthetase-like catalytic
                     core domain. Class II amino acyl-tRNA synthetases (aaRS)
                     share a common fold and generally attach an amino acid to
                     the 3' OH of ribose of the appropriate tRNA. PheRS is an
                     exception in that it attaches...; cl00268"
                     /db_xref="CDD:444800"
     CDS             1..136
                     /gene="LOC144131081"
                     /coded_by="XM_077664439.1:87..497"
                     /db_xref="GeneID:144131081"
ORIGIN      
        1 msastawvvq mrkstetaka ispsmcryqk ahiktrnvve rtfgvwkrrf pcldmglqhk
       61 pkqaavitaf aalqnfaclm kepqppiesa piqdappart sttaarrpeh lppvdavsds
      121 lsgmqaprvl iqksft
//
LOCUS       XP_077520566             192 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144131129 [Amblyomma americanum].
ACCESSION   XP_077520566
VERSION     XP_077520566.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664440.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..192
                     /product="uncharacterized protein LOC144131129"
                     /calculated_mol_wt=21564
     CDS             1..192
                     /gene="LOC144131129"
                     /coded_by="XM_077664440.1:426..1004"
                     /db_xref="GeneID:144131129"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedrrr rhlnrrltat mkrlsgvhvl
       61 nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar pgevyvvhrc
      121 rrcgakghkt dhcgptahra atplqvavev llqtalfrat slfpgrsras rfvpvfplcr
      181 nrrqtsksrg vs
//
LOCUS       XP_077520567             207 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144131172 [Amblyomma americanum].
ACCESSION   XP_077520567
VERSION     XP_077520567.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664441.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..207
                     /product="uncharacterized protein LOC144131172"
                     /calculated_mol_wt=22598
     CDS             1..207
                     /gene="LOC144131172"
                     /coded_by="XM_077664441.1:100..723"
                     /db_xref="GeneID:144131172"
ORIGIN      
        1 mlgpftldnl ryvysikrrh yhdelkdkta rtksgeleyt grwkffdcls flcvhsepfr
       61 aavaspmlhf eeeqlielee esdgtllddg dtsvveaees silpdsilpt adalrvaakd
      121 eppatpstsl eyalstfppp lknarasssa vaapasslav qlliqtpsds csssvvprve
      181 nvqslsspvv gyydecdqfg nivanym
//
LOCUS       XP_077520569             146 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144131260 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077520569
VERSION     XP_077520569.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664443.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..146
                     /product="uncharacterized protein LOC144131260 isoform X1"
                     /calculated_mol_wt=15848
     CDS             1..146
                     /gene="LOC144131260"
                     /coded_by="XM_077664443.1:310..750"
                     /db_xref="GeneID:144131260"
ORIGIN      
        1 mnrmaavvpv ilrarnssqa rdpthrppfa altsvlccqr pasigpcvlp afgcvippdp
       61 fmlktksrme vvqatklcvi fardleqagk lvdgqwysls kasffsktkt iflgswamyl
      121 gtcgqdnagq hasasskkkh etvnky
//
LOCUS       XP_077520570             140 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144131260 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077520570
VERSION     XP_077520570.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664444.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..140
                     /product="uncharacterized protein LOC144131260 isoform X2"
                     /calculated_mol_wt=15194
     CDS             1..140
                     /gene="LOC144131260"
                     /coded_by="XM_077664444.1:317..739"
                     /db_xref="GeneID:144131260"
ORIGIN      
        1 mnrmaavvpv ilrarnssqa rdpthrppfa altsvlccqr pasigpcvlp afgcvippdp
       61 fmlktksrme vvqatklcvi fardleqagk lvdgqwyslt ktktiflgsw amylgtcgqd
      121 nagqhasass kkkhetvnky
//
LOCUS       XP_077520571             137 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144131318 [Amblyomma americanum].
ACCESSION   XP_077520571
VERSION     XP_077520571.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664445.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..137
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..137
                     /product="uncharacterized protein LOC144131318"
                     /calculated_mol_wt=15054
     Region          15..97
                     /region_name="DUF4806"
                     /note="Domain of unknown function (DUF4806); pfam16064"
                     /db_xref="CDD:465001"
     CDS             1..137
                     /gene="LOC144131318"
                     /coded_by="XM_077664445.1:77..490"
                     /db_xref="GeneID:144131318"
ORIGIN      
        1 magrlsrdvv vsreqnplit tpfkdyealk efdvtlhgsk redfiqelsg kgganvtsst
       61 rrllrhlled slacqfswlg kkgkhafndl rlcsclisai krghdandyv vgaaihewlr
      121 haparhcsat gknnavg
//
LOCUS       XP_077520572             505 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144131349 [Amblyomma americanum].
ACCESSION   XP_077520572
VERSION     XP_077520572.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664446.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..505
                     /product="uncharacterized protein LOC144131349"
                     /calculated_mol_wt=53937
     Region          29..>103
                     /region_name="ASH"
                     /note="Abnormal spindle-like microcephaly-assoc'd,
                     ASPM-SPD-2-Hydin; pfam15780"
                     /db_xref="CDD:464865"
     CDS             1..505
                     /gene="LOC144131349"
                     /coded_by="XM_077664446.1:7..1524"
                     /db_xref="GeneID:144131349"
ORIGIN      
        1 magklltfsp pskvpskvve gravdaevpi lllqpfsksp qvtfeqipvg qeavrqlkvs
       61 npsntdhmvt lsdtlrskgf radpevfkll pgqsqlvsff wkpngnetss rlavpistdk
      121 gykgrfvllg tvreepkplk pprknlvkss iltssqkpnl ssrattsvqt pkkvnstpar
      181 ackkvvppir atipkefkss anknagrenl tskvsasaqv skkaatamle pckktvppvr
      241 vtvpkcfasr pnknatsnrp assslpasit cdpliggdaq sllgggtisv vpasnksggl
      301 yplktatcte eirremfacg apaleatanv tvedsedsfk tanatfensl emsknafnds
      361 lepgkladst avdaaatvnn seehsdfgng nndtfvldls aemlyqqiie kqscassqke
      421 epittsaeqa kpllctpqea diegslwppq lqaisneqrs sspkcpakas kedslpdien
      481 lltelelvag rdprcsvdds lleml
//
LOCUS       XP_077520573             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144131366 [Amblyomma americanum].
ACCESSION   XP_077520573
VERSION     XP_077520573.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664447.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144131366"
                     /calculated_mol_wt=15118
     CDS             1..133
                     /gene="LOC144131366"
                     /coded_by="XM_077664447.1:355..756"
                     /db_xref="GeneID:144131366"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedrrr rhlnrrltat mkrlsgvhvl
       61 nhehrfldas gepmlslfaa drgidqmsqi ivaalvkiyg pgiasasrar pgevyvvhrc
      121 rrcgakghkt dhc
//
LOCUS       XP_077520574             438 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144131382 [Amblyomma americanum].
ACCESSION   XP_077520574
VERSION     XP_077520574.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664448.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..438
                     /product="uncharacterized protein LOC144131382"
                     /calculated_mol_wt=46688
     CDS             1..438
                     /gene="LOC144131382"
                     /coded_by="XM_077664448.1:51..1367"
                     /db_xref="GeneID:144131382"
ORIGIN      
        1 mvtlsdtlrs kgfradpevf kllpgqsqlv sffwkpngne tssrlavpis tdkgykgrfv
       61 llgtvreepk plkpprknlv kssiltssqk pnlssratts vqtpkkvnst parackkavp
      121 piratipkef kssanknagr enltskvsas aqvskkaata mlepckktvp pvrvtvpkcf
      181 asrpnknats nrpassslpa sitcdpligg daqsllgggt isvvpasnks gglyplktat
      241 cteeirremf acgapaleat anvtvedsed sfktanatfe nslemsknaf ndslepgkla
      301 dstavdaaat vnnseehsdf gngnndtfvl dlsaemlyqq iiekqscass qkeepittsa
      361 eqakpllctp qeadiegslw ppqlqaisne qrssspkcpa kaskedslpd ienlltelel
      421 vagrdprcsv ddslleml
//
LOCUS       XP_077520575             137 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144131398 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077520575
VERSION     XP_077520575.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664449.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..137
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..137
                     /product="uncharacterized protein LOC144131398 isoform X1"
                     /calculated_mol_wt=15054
     Region          15..97
                     /region_name="DUF4806"
                     /note="Domain of unknown function (DUF4806); pfam16064"
                     /db_xref="CDD:465001"
     CDS             1..137
                     /gene="LOC144131398"
                     /coded_by="XM_077664449.1:77..490"
                     /db_xref="GeneID:144131398"
ORIGIN      
        1 magrlsrdvv vsreqnplit tpfkdyealk efdvtlhgsk redfiqelsg kgganvtsst
       61 rrllrhlled slacqfswlg kkgkhafndl rlcsclisai krghdandyv vgaaihewlr
      121 haparhcsat gknnavg
//
LOCUS       XP_077520576             117 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144131398 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077520576
VERSION     XP_077520576.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664450.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..117
                     /product="uncharacterized protein LOC144131398 isoform X2"
                     /calculated_mol_wt=12876
     CDS             1..117
                     /gene="LOC144131398"
                     /coded_by="XM_077664450.1:660..1013"
                     /db_xref="GeneID:144131398"
ORIGIN      
        1 mmrgllhqav spdhrpaqls dedpkmllet dpmlgvtskl itdnqlsqkq glplpasqga
       61 vehwsrrklm lpedsrtfge rwsecnline ttpaapsggq pgmpvfvagq kretrfq
//
LOCUS       XP_077520577             148 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144131424 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077520577
VERSION     XP_077520577.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664451.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="uncharacterized protein LOC144131424 isoform X1"
                     /calculated_mol_wt=16759
     CDS             1..148
                     /gene="LOC144131424"
                     /coded_by="XM_077664451.1:421..867"
                     /db_xref="GeneID:144131424"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedrrr rhlnrrltat mkrlsgvhvl
       61 nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar pgevyvvhrc
      121 rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077520578              98 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144131424 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077520578
VERSION     XP_077520578.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664452.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..98
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..98
                     /product="uncharacterized protein LOC144131424 isoform X2"
                     /calculated_mol_wt=10604
     CDS             1..98
                     /gene="LOC144131424"
                     /coded_by="XM_077664452.1:500..796"
                     /db_xref="GeneID:144131424"
ORIGIN      
        1 mkrlsgvhvl nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar
       61 pgevyvvhrc rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077520579             224 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144131466 [Amblyomma americanum].
ACCESSION   XP_077520579
VERSION     XP_077520579.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664453.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..224
                     /product="uncharacterized protein LOC144131466"
                     /calculated_mol_wt=24356
     Region          88..108
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          88..108
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Site            order(88,91,104,108)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          <113..220
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          116..136
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(116,119,132,136)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(121,123,125,127..128,131..132,135,149,151,155..156,
                     159..160,163,177,179,181,183..184,187..188,191)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          144..164
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          172..192
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          200..220
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..224
                     /gene="LOC144131466"
                     /coded_by="XM_077664453.1:125..799"
                     /db_xref="GeneID:144131466"
ORIGIN      
        1 msvnlsmnmt mgvagwepsy aqwgpppyhh hpqeelyasp gyaadmaapp pppppplphq
       61 vapggqvaaa gqgapargsa adgrvnlcgi cgkayarpst lkthlrthsg erpyrclqcs
      121 kcfsqaanlt ahlrthsgek pfrcpvcerr fsqsssvtth mrthsgerpy rcclckkafs
      181 dsstltkhlr thsgekpyqc rlcllrfsqs gnlnrhmrvh anas
//
LOCUS       XP_077520580             209 aa            linear   INV 25-NOV-2025
DEFINITION  homeobox protein MSX-1-like [Amblyomma americanum].
ACCESSION   XP_077520580
VERSION     XP_077520580.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664454.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..209
                     /product="homeobox protein MSX-1-like"
                     /calculated_mol_wt=22731
     Region          95..151
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..209
                     /gene="LOC144131498"
                     /coded_by="XM_077664454.1:35..664"
                     /db_xref="GeneID:144131498"
ORIGIN      
        1 mtglqpakkl tpfsvealla dvvtsgprdv qeaqvtssss naepdlclpp eqalhwgaaw
       61 ftrsppapgh leapggpckl papvkcqlrk hksnrkprtp fttqqllsle rkfrakqyls
      121 iaeraefsss lsltetqvki wfqnrrakek rlkeaeierl rmaarppvlp ffgpfgalca
      181 apgaglhapv fapavfappp ppperqppp
//
LOCUS       XP_077520581             225 aa            linear   INV 25-NOV-2025
DEFINITION  profilin-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077520581
VERSION     XP_077520581.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664455.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..225
                     /product="profilin-like isoform X1"
                     /calculated_mol_wt=23254
     Region          95..222
                     /region_name="Profilin"
                     /note="pfam00235"
                     /db_xref="CDD:459724"
     CDS             1..225
                     /gene="LOC144131526"
                     /coded_by="XM_077664455.1:16..693"
                     /db_xref="GeneID:144131526"
ORIGIN      
        1 mhsgpsthds rngahadrar stsavtrlel gvpgaslgva aaaaatssst aardaavats
       61 acsnrapsar parsaaswpg vcricccskv tlrkmswqay vdnqicaqvq ctvaaiaals
      121 ngaiwakyek dpnvtvsqqe lqaiadtmrn nptnfnengi fiagtkyvcl sadnslvrgr
      181 kgtsafiava tktcllvaat vdgfppgqln tvveklgdyl ksngy
//
LOCUS       XP_077520582             131 aa            linear   INV 25-NOV-2025
DEFINITION  profilin-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077520582
VERSION     XP_077520582.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664456.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..131
                     /product="profilin-like isoform X2"
                     /calculated_mol_wt=13923
     Region          1..128
                     /region_name="Profilin"
                     /note="pfam00235"
                     /db_xref="CDD:459724"
     CDS             1..131
                     /gene="LOC144131526"
                     /coded_by="XM_077664456.1:190..585"
                     /db_xref="GeneID:144131526"
ORIGIN      
        1 mswqayvdnq icaqvqctva aiaalsngai wakyekdpnv tvsqqelqai adtmrnnptn
       61 fnengifiag tkyvclsadn slvrgrkgts afiavatktc llvaatvdgf ppgqlntvve
      121 klgdylksng y
//
LOCUS       XP_077520583             150 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144131579 [Amblyomma americanum].
ACCESSION   XP_077520583
VERSION     XP_077520583.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664457.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..150
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..150
                     /product="uncharacterized protein LOC144131579"
                     /calculated_mol_wt=16149
     CDS             1..150
                     /gene="LOC144131579"
                     /coded_by="XM_077664457.1:459..911"
                     /db_xref="GeneID:144131579"
ORIGIN      
        1 maswtvsysd atrhhpqrti iqppsnkyis fwllgslsaa llllvvllgc ctrrlahrar
       61 rasreqvhvl pggnaaprlp pgrprlpvgd phsreillcp acrsaqeemf qtakaapaaa
      121 sparwspppp yspsptllvq phtagahshl
//
LOCUS       XP_077520584             150 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144131579 [Amblyomma americanum].
ACCESSION   XP_077520584
VERSION     XP_077520584.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664458.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..150
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..150
                     /product="uncharacterized protein LOC144131579"
                     /calculated_mol_wt=16149
     CDS             1..150
                     /gene="LOC144131579"
                     /coded_by="XM_077664458.1:561..1013"
                     /db_xref="GeneID:144131579"
ORIGIN      
        1 maswtvsysd atrhhpqrti iqppsnkyis fwllgslsaa llllvvllgc ctrrlahrar
       61 rasreqvhvl pggnaaprlp pgrprlpvgd phsreillcp acrsaqeemf qtakaapaaa
      121 sparwspppp yspsptllvq phtagahshl
//
LOCUS       XP_077520585             468 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144131616 [Amblyomma americanum].
ACCESSION   XP_077520585
VERSION     XP_077520585.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664459.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..468
                     /product="uncharacterized protein LOC144131616"
                     /calculated_mol_wt=50858
     Region          3..427
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          38..77
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          83..117
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          122..223
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          230..273
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          279..315
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          322..355
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          361..383
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..468
                     /gene="LOC144131616"
                     /coded_by="XM_077664459.1:44..1450"
                     /db_xref="GeneID:144131616"
ORIGIN      
        1 mgsacstrcr kkaasagdae avrwphlser leqhrgginc lalsedhsll vsgsedgrvl
       61 lwssqstpve llgtlsgheg yvthctvhgn yvvtgsadgt vrkwsivdak cvhvfhghga
      121 rvnrvlctgd lvistshdkt arawrfnadl dvqemeerrl lrqvassgrs srgssatkkk
      181 sdsrastaee dseddgstss gfseeadsri rtgevidrit vfrghtksvf pvvfvpadgl
      241 saadvedivv tgshdctart wglfsgeclk ilrghsapin amvvdaegtf vftgggdgvi
      301 rswhvrsgec qhvltghqgp ivcllahskm lysgssdnta rawvmefgec trvyrghqht
      361 vdclcyhdrm lytgsgdkva rmfeaksgtl krsfrghehg vvcievvksk lftgsydgsl
      421 fvwdttgvvd etvfgdqale ededddlped seevkmavnf lepfiheg
//
LOCUS       XP_077520586             275 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144131643 [Amblyomma americanum].
ACCESSION   XP_077520586
VERSION     XP_077520586.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664460.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..275
                     /product="uncharacterized protein LOC144131643"
                     /calculated_mol_wt=30969
     Region          80..240
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..275
                     /gene="LOC144131643"
                     /coded_by="XM_077664460.1:1..828"
                     /db_xref="GeneID:144131643"
ORIGIN      
        1 mpfedqillt lmrlrlglll ndlslhfgas taaisrifcf ivtklakfar eflvfwlprt
       61 airrtlpyvf eenysvtciv dcfeifidrp gqlhrrntty ssykshntak ilhviapsgf
      121 imslskpydg rasdrfitld sgflnhltqg devladrgfn iddllppsvk vslpsfskgr
      181 pqicrsdvvs srrlarlrih versirrlkc frilkqfpas iftkkpfand vlvaiaglcn
      241 lqpnlirdhg dasepgaqsp shpgkqlevv dntpe
//
LOCUS       XP_077520588              66 aa            linear   INV 25-NOV-2025
DEFINITION  kelch-like protein 20 [Amblyomma americanum].
ACCESSION   XP_077520588
VERSION     XP_077520588.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664462.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..66
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..66
                     /product="kelch-like protein 20"
                     /calculated_mol_wt=7391
     Region          <1..33
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     CDS             1..66
                     /gene="LOC144131750"
                     /coded_by="XM_077664462.1:22..222"
                     /db_xref="GeneID:144131750"
ORIGIN      
        1 mrrefllfkv geepllrtse egkellieam ryhlmpewra amagprtsak qlaprdadth
       61 arpsga
//
LOCUS       XP_077520589              66 aa            linear   INV 25-NOV-2025
DEFINITION  kelch-like protein 20 [Amblyomma americanum].
ACCESSION   XP_077520589
VERSION     XP_077520589.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664463.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..66
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..66
                     /product="kelch-like protein 20"
                     /calculated_mol_wt=7391
     Region          <1..33
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     CDS             1..66
                     /gene="LOC144131766"
                     /coded_by="XM_077664463.1:48..248"
                     /db_xref="GeneID:144131766"
ORIGIN      
        1 mrrefllfkv geepllrtse egkellieam ryhlmpewra amagprtsak qlaprdadth
       61 arpsga
//
LOCUS       XP_077520590             174 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144131809 [Amblyomma americanum].
ACCESSION   XP_077520590
VERSION     XP_077520590.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664464.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..174
                     /product="uncharacterized protein LOC144131809"
                     /calculated_mol_wt=18876
     CDS             1..174
                     /gene="LOC144131809"
                     /coded_by="XM_077664464.1:145..669"
                     /db_xref="GeneID:144131809"
ORIGIN      
        1 mvsmyiiflc ivplaviisf styyfqchlk twwmtnarka aikshaqqvg ldrrggrpqs
       61 kfnidaetik alnisapvtq pmvpqqplap stppmppsqh qkshrsvsrv rgsfrnvdis
      121 gpcaaelkqq pcgstrpapa rpaparpaqg gprvsrglic vhhksvclii vhga
//
LOCUS       XP_077520591             344 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144131825 [Amblyomma americanum].
ACCESSION   XP_077520591
VERSION     XP_077520591.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664465.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..344
                     /product="uncharacterized protein LOC144131825"
                     /calculated_mol_wt=38502
     Region          65..157
                     /region_name="GT1"
                     /note="myb-like, SANT family; cl23759"
                     /db_xref="CDD:474047"
     CDS             1..344
                     /gene="LOC144131825"
                     /coded_by="XM_077664465.1:72..1106"
                     /db_xref="GeneID:144131825"
ORIGIN      
        1 mrkkrrpcsr lascedsgva palpdgsmqq psssgaaqls aafeqrlavv kpahtdesla
       61 elfaliesnp clwkndsrsf knlrlkrrlw drfaghlqkr fpmlgpftpd nlryvysikr
      121 rqyydelkdk tartksgele ytgrwkffdc lsflrvhsep fraavvspml hfekeqliel
      181 eeesdetlld dgdtsvveae essilpdsil ptadalrvaa ndeppatpst sleyalstsp
      241 pplkkarass savaapassv avqpliqtpl dscsstvmpr venvqslssp vggyydecdq
      301 fgnivanymr rlseedrlef hchimsctkd ffkcfsrfgv krrl
//
LOCUS       XP_077520592             344 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144131841 [Amblyomma americanum].
ACCESSION   XP_077520592
VERSION     XP_077520592.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664466.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..344
                     /product="uncharacterized protein LOC144131841"
                     /calculated_mol_wt=38482
     Region          65..157
                     /region_name="GT1"
                     /note="myb-like, SANT family; cl23759"
                     /db_xref="CDD:474047"
     CDS             1..344
                     /gene="LOC144131841"
                     /coded_by="XM_077664466.1:84..1118"
                     /db_xref="GeneID:144131841"
ORIGIN      
        1 mrkkrrpcsr lascedsgva palpdgsmqq psssgaaqls aafeqrlavv kpahtdesla
       61 elfaliesnp clwkndsrsf knlrlkrrlw drfaghlqkr fpmlgpftpd nlryvysikr
      121 rqyydelkdk tartksgele ytgrwkffdc lsflrvhsep fraavvspml hfekeqliel
      181 eeesdethld dgdtsvveae essilpdsil ptadalrvaa neeppatpst sleyalstsp
      241 pplkkarass savaapassv avqpliqtps dscsstvvpr venvqslssp vggyydecdq
      301 fgnivanymr rlseedrlef hchimsctkd ffkcfsrfgv krrl
//
LOCUS       XP_077520593             344 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144131873 [Amblyomma americanum].
ACCESSION   XP_077520593
VERSION     XP_077520593.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664467.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..344
                     /product="uncharacterized protein LOC144131873"
                     /calculated_mol_wt=38430
     Region          65..157
                     /region_name="GT1"
                     /note="myb-like, SANT family; cl23759"
                     /db_xref="CDD:474047"
     CDS             1..344
                     /gene="LOC144131873"
                     /coded_by="XM_077664467.1:59..1093"
                     /db_xref="GeneID:144131873"
ORIGIN      
        1 mrkkrrpcsr lascedsgva palpdgsmqq psssgaaqls aafeqrlavv kpahtdesla
       61 elfaliesnp clwkndsrsf knlrlkrrlw drfaghlqkr fpmlgpftpd nlryvysikr
      121 rqyydelkdk tartksgele ytgrwkffdc lsflrvhsep fraavvspml hfekeqliel
      181 eeesdetlld dgdtsvveae esstlpdsil ptadalrvaa neeppatpst sleyalstsp
      241 pplkkarasa savaapassv avqpliqtps dscsstvvpr venvqslssp vggyydecdq
      301 fgnivanymr rlseedrlef hchimsctkd ffkcfsrfgv krrl
//
LOCUS       XP_077520594             213 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144131889 [Amblyomma americanum].
ACCESSION   XP_077520594
VERSION     XP_077520594.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664468.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..213
                     /product="uncharacterized protein LOC144131889"
                     /calculated_mol_wt=23650
     Region          67..88
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(67,70,83,88)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          95..115
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(95,98,111,115)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(100,102,104,106..107,110..111,114,128,130,134..135,
                     138..139,142,156,158,160,162..163,166..167,170)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          108..132
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          123..143
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          147..>209
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          151..171
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          179..200
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..213
                     /gene="LOC144131889"
                     /coded_by="XM_077664468.1:215..856"
                     /db_xref="GeneID:144131889"
ORIGIN      
        1 mwlccrwvsd prayvppcpp yhqphnqfma ppvdpcfmgt pqpvpqavpa aaaagppgld
       61 agtgfwcdlc gkvfdrrqal lshgcrshgi crfvcthcga afrqsgqltk hnrthtgerp
      121 fpcqvcqrsf tqksnlvehv rthtgerpyr chicqatfsr kahlvghtrt htgerpfqcp
      181 acpkafatss alnqhrrvth rspendnspr pll
//
LOCUS       XP_077520595             250 aa            linear   INV 25-NOV-2025
DEFINITION  protein Skeletor, isoforms B/C-like [Amblyomma americanum].
ACCESSION   XP_077520595
VERSION     XP_077520595.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664469.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..250
                     /product="protein Skeletor, isoforms B/C-like"
                     /calculated_mol_wt=27406
     CDS             1..250
                     /gene="LOC144131905"
                     /coded_by="XM_077664469.1:1..753"
                     /db_xref="GeneID:144131905"
ORIGIN      
        1 mapilpppag grtgfggrdi wksfnitsfr aqvgptggsr gyervtgqsg wgiswwinee
       61 lipvlhverg ktytfvvegg vdasnsaryh pfyttdnskg ggskedpavl skkghrllag
      121 vvldarnkvd vskgtgryce wthktvdksd esstwedfkq tlnlqcdvgq panftwtpdk
      181 dtpdvvyyqc fthyylgwki mvtdpdqper grgkdrmesg rgedrmesga glttallpll
      241 llgvivskaa
//
LOCUS       XP_077520596             185 aa            linear   INV 25-NOV-2025
DEFINITION  receptor expression-enhancing protein 6-like [Amblyomma
            americanum].
ACCESSION   XP_077520596
VERSION     XP_077520596.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664470.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..185
                     /product="receptor expression-enhancing protein 6-like"
                     /calculated_mol_wt=20400
     Region          67..142
                     /region_name="TB2_DP1_HVA22"
                     /note="TB2/DP1, HVA22 family; pfam03134"
                     /db_xref="CDD:460820"
     CDS             1..185
                     /gene="LOC144131948"
                     /coded_by="XM_077664470.1:95..652"
                     /db_xref="GeneID:144131948"
ORIGIN      
        1 maqeineelg rvlrergvig daldgverks kvkreqivyg vavvilllfm vnilgaktam
       61 littvwpmlg siraidradv lalqkwtayw imyalvnvfl nflfvavhry fkriylikll
      121 llawcaapmq nngsniifqk vfagkifkeg pmvpggngap aaapmpppaa ggkkespdts
      181 rkmrk
//
LOCUS       XP_077520597             272 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144131964 [Amblyomma americanum].
ACCESSION   XP_077520597
VERSION     XP_077520597.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664471.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..272
                     /product="uncharacterized protein LOC144131964"
                     /calculated_mol_wt=29171
     CDS             1..272
                     /gene="LOC144131964"
                     /coded_by="XM_077664471.1:96..914"
                     /db_xref="GeneID:144131964"
ORIGIN      
        1 msrtpkaaag pnqlreelrq qllttfaakk resamgegtq avedscsaas geplkkqplp
       61 vvsatscgch kyddlhvhvt vlcpcsrptp tdkatqtdma rtdeqqrstp rgvgisvdil
      121 htsnermfsq tcshelaasv pngtpvtprl qgvpnsadis vqaledegnv siedgskeew
      181 ypahqvfnga dtsrispadr rrsvhealar vrdtlfealr dtpramaats rtaifesvfp
      241 amptsptpsp ptaqplssll rvlqassats qr
//
LOCUS       XP_077520598             515 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144131991 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077520598
VERSION     XP_077520598.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664472.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..515
                     /product="uncharacterized protein LOC144131991 isoform X1"
                     /calculated_mol_wt=54542
     CDS             1..515
                     /gene="LOC144131991"
                     /coded_by="XM_077664472.1:10..1557"
                     /db_xref="GeneID:144131991"
ORIGIN      
        1 mssserpsss sgkpraptpk gsvqslgckv rgpwwtdgga gdldyafles pnptaplrqg
       61 nepgekmvtv daqsripeva dpqhqetase kpssststad ldnapresfl pnkrkeeadg
      121 qefvglkpad tlvangaeay gvdqrsprqi nrhppralch msspspgese dttafgfaev
      181 igrnirrgkk tgitprdfdl sslgspslia avpkfpelps phkvttasae dsnskelrts
      241 hqkfpevlpt lpssahsekv prlrippnke keptngpdld slvpayspva nggeescgid
      301 qrspqqisrh asrsspehae dstglyflrl igrnitcrrr tgitscdldl sslespspaa
      361 avpeisdlas aqmvtratgq eksshevgta sqkapdeppt lasrtdsekg shasfarske
      421 nepaamtglg gmgpsthpaa tiepvpgvdq gnsrptkrqg aktsrrrgar retsedgtts
      481 svasstvdge svarstsgrp lrsaaigkny resdi
//
LOCUS       XP_077520599             365 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144131991 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077520599
VERSION     XP_077520599.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664473.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..365
                     /product="uncharacterized protein LOC144131991 isoform X2"
                     /calculated_mol_wt=38402
     CDS             1..365
                     /gene="LOC144131991"
                     /coded_by="XM_077664473.1:10..1107"
                     /db_xref="GeneID:144131991"
ORIGIN      
        1 mssserpsss sgkpraptpk gsvqslgckv rgpwwtdgga gdldyafles pnptaplrqg
       61 nepgekmvtv daqsripeva dpqhqetase kpssststad ldnapresfl pnkrkeeadg
      121 qefvglkpad tlvangaeay gvdqrsprqi nrhppralch msspspgese dttafdflrl
      181 igrnitcrrr tgitscdldl sslespspaa avpeisdlas aqmvtratgq eksshevgta
      241 sqkapdeppt lasrtdsekg shasfarske nepaamtglg gmgpsthpaa tiepvpgvdq
      301 gnsrptkrqg aktsrrrgar retsedgtts svasstvdge svarstsgrp lrsaaigkny
      361 resdi
//
LOCUS       XP_077520600              73 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier organic anion transporter family member 4A1-like
            [Amblyomma americanum].
ACCESSION   XP_077520600
VERSION     XP_077520600.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664474.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..73
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..73
                     /product="solute carrier organic anion transporter family
                     member 4A1-like"
                     /calculated_mol_wt=7566
     Region          <1..>59
                     /region_name="OATP"
                     /note="Organic Anion Transporter Polypeptide (OATP)
                     family; pfam03137"
                     /db_xref="CDD:460821"
     CDS             1..73
                     /gene="LOC144132018"
                     /coded_by="XM_077664474.1:22..243"
                     /db_xref="GeneID:144132018"
ORIGIN      
        1 miaipsacgg tllggylidk ldltcaniir msvfasmftw lvmgfvlfhc pnssfagmsf
       61 taaaaslarc qal
//
LOCUS       XP_077520601              91 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier organic anion transporter family member 4A1-like
            [Amblyomma americanum].
ACCESSION   XP_077520601
VERSION     XP_077520601.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664475.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..91
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..91
                     /product="solute carrier organic anion transporter family
                     member 4A1-like"
                     /calculated_mol_wt=10082
     Region          <2..>57
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     CDS             1..91
                     /gene="LOC144132050"
                     /coded_by="XM_077664475.1:13..288"
                     /db_xref="GeneID:144132050"
ORIGIN      
        1 miaipsacge tllggylidk ldltcaniir msvfasmltw lvmgfvlfnc pnssfagmsf
       61 twlglrhrrr etghqgllch qpighhreid h
//
LOCUS       XP_077520603             306 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132077, partial [Amblyomma
            americanum].
ACCESSION   XP_077520603
VERSION     XP_077520603.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664477.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         <1..306
                     /product="uncharacterized protein LOC144132077"
     Region          60..111
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          141..300
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..306
                     /gene="LOC144132077"
                     /coded_by="XM_077664477.1:<1..921"
                     /db_xref="GeneID:144132077"
ORIGIN      
        1 kavhyskera aeaeqnvqqg rfsanciteq cvsvytglps kalflffvsl fknvalyipn
       61 hctvadavlv vlmklrlall nidishrfgl settvarimv knipiiasel krfiiwpsre
      121 dacrtmpmmv rrkyptcmai idctevriqk plgysarskt yshykssnti kfmvsitpcg
      181 aisfvtkcwg grasdkeiil ksgfldklqe rdivladrgf liredvahrg arlevpaltr
      241 gkrklsakev edarhisrvr ihveraigri nvfhilrdrl sitmlrhatd ivvicaaitn
      301 mkprla
//
LOCUS       XP_077520604             615 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier organic anion transporter family member 4A1-like
            [Amblyomma americanum].
ACCESSION   XP_077520604
VERSION     XP_077520604.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664478.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..615
                     /product="solute carrier organic anion transporter family
                     member 4A1-like"
                     /calculated_mol_wt=66100
     Region          48..587
                     /region_name="OATP"
                     /note="Organic Anion Transporter Polypeptide (OATP)
                     family; pfam03137"
                     /db_xref="CDD:460821"
     CDS             1..615
                     /gene="LOC144132115"
                     /coded_by="XM_077664478.1:564..2411"
                     /db_xref="GeneID:144132115"
ORIGIN      
        1 mirkshlspp pgapeedvac gwggvqppcl qplrtpqaal aafcsaaflq gltvngyvnv
       61 vlptiekrfq lrsvqagsii slynvgsllc stpvayygst rhkprimaag tlvmatgslv
      121 faaphfaaps yhtrmdvrdt cpnelsaaal cplgqgnlad yrflfmmgnf lhgcgaspfy
      181 tlgvayldds vptktsavyl giyfamailg pavgfisggy flslytditr tdvkitrfsk
      241 vwvgawwvgf vaaaclallv aiplsafpke lpgarelraa rkqeagdhgl priglekreq
      301 ffamvidllt nrtyvlltia gtietmmasg isafitkile sqfgltssaa atllgmiaip
      361 sacggtllgg ylidkldltc aniirmsvfa smftwlvmgf vlfhcpnssf agmsftkgyh
      421 fdpnaacnah ckcseilysp vcgadnvtyy spcfagcrre nivqgtvvys dcscianvre
      481 kdvlsvqael trcqttckal vpfsvglfla lfgtflnsap gmsatirtvg eptkpvalgl
      541 qwvsvrlfgt ilapilfgsi idrscltwqg lcggqrgtci iyenssmsyn lfgfliflkt
      601 lsvvfffaaw vtirn
//
LOCUS       XP_077520605             461 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132131 [Amblyomma americanum].
ACCESSION   XP_077520605
VERSION     XP_077520605.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664479.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..461
                     /product="uncharacterized protein LOC144132131"
                     /calculated_mol_wt=51592
     Region          39..>138
                     /region_name="SWIM"
                     /note="Uncharacterized protein, contains SWIM-type Zn
                     finger domain [Function unknown]; COG4715"
                     /db_xref="CDD:443750"
     Region          253..450
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(316,355,369,371)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..461
                     /gene="LOC144132131"
                     /coded_by="XM_077664479.1:7..1392"
                     /db_xref="GeneID:144132131"
ORIGIN      
        1 mnnadsgdri svalplssin cyvdkacetg rclieavday yaghvvecsi eaagngrfnf
       61 lafvsqttal nnaphevkit vcnsevdrar csckagnykc khivasllhi ngartfdrls
      121 ptdqpqkwgk aqkdrikqky epramvdlpc tkkakikepa qpegnilgrl lknlghqsaa
      181 kmpsesttei avadcgenpd rcepeprdit lmgtlhelrr llgncdlqkl lehinnarsc
      241 ediaaieqst ghqaksglww qhrvgmitas iaysvftrvk tlrtkmgphd lrpllkkvmr
      301 qtnvctaarr rgsmleesak kcyaqqqqsq hkdlivtqcg livmggrpyi garpdglvqc
      361 eccpkrvlev kcpeslekfv meitekndkt tteklkrast yfcqiqvqmg laglkqgelf
      421 vflndeknmc ipvpfdeyfr deverstyff keyvlphilc v
//
LOCUS       XP_077520606             137 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132163 [Amblyomma americanum].
ACCESSION   XP_077520606
VERSION     XP_077520606.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664480.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..137
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..137
                     /product="uncharacterized protein LOC144132163"
                     /calculated_mol_wt=14935
     Region          <48..100
                     /region_name="GH18_chitinase-like"
                     /note="The GH18 (glycosyl hydrolase, family 18) type II
                     chitinases hydrolyze chitin, an abundant polymer of
                     beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a
                     major component of the cell wall of fungi and the
                     exoskeleton of arthropods. Chitinases have...; cl10447"
                     /db_xref="CDD:471972"
     CDS             1..137
                     /gene="LOC144132163"
                     /coded_by="XM_077664480.1:1351..1764"
                     /db_xref="GeneID:144132163"
ORIGIN      
        1 msmhtrcplt tcmrctraqi cleissdlsv plykeadcki avnrdkgqwi gydnkatiln
       61 nvlfakrmrm ggicvvtvdm dyngdcsqrn vllgqlhnal nemdrpikrl sanlslpesa
      121 sssttatsrs taamast
//
LOCUS       XP_077520607             144 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132180 [Amblyomma americanum].
ACCESSION   XP_077520607
VERSION     XP_077520607.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664481.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..144
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..144
                     /product="uncharacterized protein LOC144132180"
                     /calculated_mol_wt=16090
     CDS             1..144
                     /gene="LOC144132180"
                     /coded_by="XM_077664481.1:44..478"
                     /db_xref="GeneID:144132180"
ORIGIN      
        1 mestmtplde kqhsvdechv skgamrgiqn tdplwfgglt ldpqsvkqsr spqevcgltg
       61 qrkaarvepd reewrirvpe pdgalvvqlw rhtlfeatgl rprrlptpfr pwrhflgkkt
      121 spawnrllad sggadgatah aqyl
//
LOCUS       XP_077520608             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132218 [Amblyomma americanum].
ACCESSION   XP_077520608
VERSION     XP_077520608.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664482.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144132218"
                     /calculated_mol_wt=17606
     Region          1..>60
                     /region_name="SanA"
                     /note="Uncharacterized periplasmic protein SanA, affects
                     membrane permeability for vancomycin [Cell
                     wall/membrane/envelope biogenesis]; COG2949"
                     /db_xref="CDD:442192"
     CDS             1..158
                     /gene="LOC144132218"
                     /coded_by="XM_077664482.1:397..873"
                     /db_xref="GeneID:144132218"
ORIGIN      
        1 mrkalstckk tlvaiwaahs ylapvlslqi vsmqtstcvs elisdlpptp mvvvlgtsif
       61 dcdctcivtl dgakcfegls flaametlya ayfclnmkyn qevqatlhfi qrqlvgmnpe
      121 egsrqlnrri tsvhpkvhsa lqqldkfkkk wqdcmsri
//
LOCUS       XP_077520624             185 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132243 [Amblyomma americanum].
ACCESSION   XP_077520624
VERSION     XP_077520624.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664498.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..185
                     /product="uncharacterized protein LOC144132243"
                     /calculated_mol_wt=20975
     Region          1..81
                     /region_name="RT_like"
                     /note="Reverse transcriptase (RT, RNA-dependent DNA
                     polymerase)_like family. An RT gene is usually indicative
                     of a mobile element such as a retrotransposon or
                     retrovirus. RTs occur in a variety of mobile elements,
                     including retrotransposons, retroviruses, group...;
                     cd00304"
                     /db_xref="CDD:238185"
     Site            2
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     CDS             1..185
                     /gene="LOC144132243"
                     /coded_by="XM_077664498.1:3..560"
                     /db_xref="GeneID:144132243"
ORIGIN      
        1 mgasisvtma nlavedvesr alatfnpepk lflryvddcf cvvktseven llihlnstep
       61 aiqftvereq qgslpfldvl vtrhdkglrf svyrkpthtg rylhfnsnhp tpqkasvits
      121 llkradtics sheeksnekn rvisdlrkne yrssfirrvs qkqkktalfl pqahartgnv
      181 fayht
//
LOCUS       XP_077520636             143 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132250 [Amblyomma americanum].
ACCESSION   XP_077520636
VERSION     XP_077520636.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664510.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..143
                     /product="uncharacterized protein LOC144132250"
                     /calculated_mol_wt=15681
     CDS             1..143
                     /gene="LOC144132250"
                     /coded_by="XM_077664510.1:153..584"
                     /db_xref="GeneID:144132250"
ORIGIN      
        1 msemaspcee diraaaaead esldatlvad gseasqsdds vsvesapsss atmtpekatt
       61 drslsdgrlv raaepdlpea lvrrledrii drtrhlddhl rdmhielslt qhtfmgemqt
      121 tlqevfdevl alrtiidemr qpi
//
LOCUS       XP_077520663             112 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132258 [Amblyomma americanum].
ACCESSION   XP_077520663
VERSION     XP_077520663.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664537.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..112
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..112
                     /product="uncharacterized protein LOC144132258"
                     /calculated_mol_wt=11847
     CDS             1..112
                     /gene="LOC144132258"
                     /coded_by="XM_077664537.1:226..564"
                     /db_xref="GeneID:144132258"
ORIGIN      
        1 mdaswayakg yarprkkfrl sdfmpvlads slslstgpsf rksqmaspce ediraaaasa
       61 aaaaagsnsa hrtrtlgeqp qdttavavft falvlknnga rtflvvsvyd eq
//
LOCUS       XP_077520751             364 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132307 [Amblyomma americanum].
ACCESSION   XP_077520751
VERSION     XP_077520751.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664625.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..364
                     /product="uncharacterized protein LOC144132307"
                     /calculated_mol_wt=37822
     CDS             1..364
                     /gene="LOC144132307"
                     /coded_by="XM_077664625.1:160..1254"
                     /db_xref="GeneID:144132307"
ORIGIN      
        1 msemaspcee diraaaaeaa vpsasggnqa tapapppagp srlsfaqalq qgvaanpqpr
       61 avnggpttaa aprarssaqs sgqpsgsrps ygaaagsaki aasspwpaaa aatvsgsqpa
      121 aaalpqslpc tppgevtest peagdqatpg ddvtvkgqcd grnlmhtswa saegnarrrk
      181 qfrlsdflpv leddwslppl estslesssl estslslstw kspmaspcee dmwaaaasaa
      241 aaeadessdd tlvangseas qshhsvsves vssssatmsp ekapedrsls dgpaepdlpe
      301 alvrrledri idrtrhlddh lrhmhtdlsl mqrtfmgemq tmlqevfdev lelrtvidem
      361 repi
//
LOCUS       XP_077520760             361 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132312 [Amblyomma americanum].
ACCESSION   XP_077520760
VERSION     XP_077520760.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664634.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..361
                     /product="uncharacterized protein LOC144132312"
                     /calculated_mol_wt=37638
     Region          <11..146
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     CDS             1..361
                     /gene="LOC144132312"
                     /coded_by="XM_077664634.1:59..1144"
                     /db_xref="GeneID:144132312"
ORIGIN      
        1 mhkkyknkgd sagrrpavps asggnqatap apspagpsrl sfaqalqqgv aanpqpravn
       61 ggpttaaapr arssakssaq psgsrpsyga aaasakvaas spwpesaaaa vsgsqpaaaa
      121 lpqslpctpp gevtestpea gdqatpgddv tvkgqcdgrn lmhtswasae gnarrrkqfr
      181 lsdflpvled dwslpplest slestslest slsvstwnsp maspceedig aaaasaaaae
      241 adeslddtlv angseasqsh hsvsvepvss ssatmtpeka tedrslsdgp aepdlpealv
      301 rrledriidr trhlddhlrh mhtelslmqr tfmgemqaml qevfdevlel rtvidemrep
      361 i
//
LOCUS       XP_077520774             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077520774
VERSION     XP_077520774.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664648.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=49633
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144132324"
                     /coded_by="XM_077664648.1:249..1580"
                     /db_xref="GeneID:144132324"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasegkyv
       61 prailvdlep gtmdavrsgq fgqlfrpdnf vfgqsgagsn wakghytega elvdsvldvv
      121 rkeaescdcm qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsmh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prfhffltgf apltsrgcqe yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgq msmrevddqm lniqnkqssh fvewipnsvk tavcdipprg
      361 lkmsatfign staiqeifrr iseqftamfr rkaflhwytg egmdeyefie aesnlndlvs
      421 eyqqyqeatp edegefeetq aea
//
LOCUS       XP_077520785             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077520785
VERSION     XP_077520785.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664659.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like isoform X1"
                     /calculated_mol_wt=49691
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144132331"
                     /coded_by="XM_077664659.1:154..1485"
                     /db_xref="GeneID:144132331"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasegkyv
       61 prailvdlep gtmdavrsgq fgqlfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaescdcm qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsmh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prfhffltgf apltsrgcqe yraltvlelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgq msmrevddqm lniqnkqssh fvewipnsvk tavcdipprg
      361 lkmsatfign staiqeifrr iseqftamfr rkaflhwytg egmdeyefie aesnlnnlvs
      421 eyqqyqestp edegefeetq aea
//
LOCUS       XP_077520788             442 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077520788
VERSION     XP_077520788.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664662.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..442
                     /product="tubulin beta-4 chain-like isoform X2"
                     /calculated_mol_wt=49562
     Region          1..442
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..442
                     /gene="LOC144132331"
                     /coded_by="XM_077664662.1:155..1483"
                     /db_xref="GeneID:144132331"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasgkyvp
       61 railvdlepg tmdavrsgqf gqlfrpdnfv fgqsgagnnw akghytegae lvdsvldvvr
      121 keaescdcmq gfqlthslgg gtgsgmgtll iskireeypd rimntysvvp spkvsdtvve
      181 pynatlsmhq lventdetyc idnealydic frtlklttpt ygdlnhlvsa tmsgvttclr
      241 fpgqlnadlr klavnmvpfp rfhffltgfa pltsrgcqey raltvleltq qmfdaknmma
      301 acdprhgryl tvaavfrgqm smrevddqml niqnkqsshf vewipnsvkt avcdipprgl
      361 kmsatfigns taiqeifrri seqftamfrr kaflhwytge gmdeyefiea esnlnnlvse
      421 yqqyqestpe degefeetqa ea
//
LOCUS       XP_077520792             406 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077520792
VERSION     XP_077520792.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664666.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..406
                     /product="tubulin beta-4 chain-like isoform X3"
                     /calculated_mol_wt=45538
     Region          1..406
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..406
                     /gene="LOC144132331"
                     /coded_by="XM_077664666.1:153..1373"
                     /db_xref="GeneID:144132331"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasgqsga
       61 gnnwakghyt egaelvdsvl dvvrkeaesc dcmqgfqlth slgggtgsgm gtlliskire
      121 eypdrimnty svvpspkvsd tvvepynatl smhqlventd etycidneal ydicfrtlkl
      181 ttptygdlnh lvsatmsgvt tclrfpgqln adlrklavnm vpfprfhffl tgfapltsrg
      241 cqeyraltvl eltqqmfdak nmmaacdprh gryltvaavf rgqmsmrevd dqmlniqnkq
      301 sshfvewipn svktavcdip prglkmsatf ignstaiqei frriseqfta mfrrkaflhw
      361 ytgegmdeye fieaesnlnn lvseyqqyqe stpedegefe etqaea
//
LOCUS       XP_077520800             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077520800
VERSION     XP_077520800.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664674.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like isoform X1"
                     /calculated_mol_wt=49676
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144132345"
                     /coded_by="XM_077664674.1:70..1401"
                     /db_xref="GeneID:144132345"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasegkyv
       61 prailvdlep gtmdavrsgq fgqlfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaescdcm qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsmh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prfhffltgf apltsrgcqe yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgq msmrevddqm lniqnkqssh fvewipnsvk tavcdipprg
      361 lkmsatfign staiqeifrr iseqftamfr rkaflhwytg egmdeyefie aesnlndlvs
      421 eyqqyqestp edegefeetq aea
//
LOCUS       XP_077520808             442 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077520808
VERSION     XP_077520808.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664682.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..442
                     /product="tubulin beta-4 chain-like isoform X2"
                     /calculated_mol_wt=49547
     Region          1..442
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..442
                     /gene="LOC144132345"
                     /coded_by="XM_077664682.1:70..1398"
                     /db_xref="GeneID:144132345"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasgkyvp
       61 railvdlepg tmdavrsgqf gqlfrpdnfv fgqsgagnnw akghytegae lvdsvldvvr
      121 keaescdcmq gfqlthslgg gtgsgmgtll iskireeypd rimntysvvp spkvsdtvve
      181 pynatlsmhq lventdetyc idnealydic frtlklttpt ygdlnhlvsa tmsgvttclr
      241 fpgqlnadlr klavnmvpfp rfhffltgfa pltsrgcqey raltvpeltq qmfdaknmma
      301 acdprhgryl tvaavfrgqm smrevddqml niqnkqsshf vewipnsvkt avcdipprgl
      361 kmsatfigns taiqeifrri seqftamfrr kaflhwytge gmdeyefiea esnlndlvse
      421 yqqyqestpe degefeetqa ea
//
LOCUS       XP_077520817             406 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077520817
VERSION     XP_077520817.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664691.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..406
                     /product="tubulin beta-4 chain-like isoform X3"
                     /calculated_mol_wt=45523
     Region          1..406
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..406
                     /gene="LOC144132345"
                     /coded_by="XM_077664691.1:70..1290"
                     /db_xref="GeneID:144132345"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasgqsga
       61 gnnwakghyt egaelvdsvl dvvrkeaesc dcmqgfqlth slgggtgsgm gtlliskire
      121 eypdrimnty svvpspkvsd tvvepynatl smhqlventd etycidneal ydicfrtlkl
      181 ttptygdlnh lvsatmsgvt tclrfpgqln adlrklavnm vpfprfhffl tgfapltsrg
      241 cqeyraltvp eltqqmfdak nmmaacdprh gryltvaavf rgqmsmrevd dqmlniqnkq
      301 sshfvewipn svktavcdip prglkmsatf ignstaiqei frriseqfta mfrrkaflhw
      361 ytgegmdeye fieaesnlnd lvseyqqyqe stpedegefe etqaea
//
LOCUS       XP_077520827             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077520827
VERSION     XP_077520827.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664701.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=49689
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144132357"
                     /coded_by="XM_077664701.1:65..1396"
                     /db_xref="GeneID:144132357"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasegkyv
       61 prailvdlep gtmdavrsgq fgqlfrpdnf vfgqsgaann wakghytega elvdsvldvv
      121 rkeaescdcm qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsmh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prfhffltgf apltsrgcqe yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgq msmrevddqm lniqnkqssh fvewipnsvk tavcdipprg
      361 lkmsatfign staiqeifrr iseqftamfr rkaflhwytg egmdeyefie aesnlnnlvs
      421 eyqqyqestp edegefeetq aea
//
LOCUS       XP_077520838             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077520838
VERSION     XP_077520838.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664712.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like isoform X1"
                     /calculated_mol_wt=49706
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144132363"
                     /coded_by="XM_077664712.1:297..1628"
                     /db_xref="GeneID:144132363"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasegkyv
       61 prailvdlep gtmdavrsgq fgqlfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rketescdcm qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsmh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prfhffltgf apltsrgcqe yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgq msmrevddqm lniqnkqssh fvewipnsvk tavcdipprg
      361 lkmsatfign staiqeifrr iseqftamfr rkaflhwytg egmdeyefie aesnlndlvs
      421 eyqqyqestp edegefeetq aea
//
LOCUS       XP_077520846             442 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077520846
VERSION     XP_077520846.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664720.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..442
                     /product="tubulin beta-4 chain-like isoform X2"
                     /calculated_mol_wt=49577
     Region          1..442
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..442
                     /gene="LOC144132363"
                     /coded_by="XM_077664720.1:299..1627"
                     /db_xref="GeneID:144132363"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasgkyvp
       61 railvdlepg tmdavrsgqf gqlfrpdnfv fgqsgagnnw akghytegae lvdsvldvvr
      121 ketescdcmq gfqlthslgg gtgsgmgtll iskireeypd rimntysvvp spkvsdtvve
      181 pynatlsmhq lventdetyc idnealydic frtlklttpt ygdlnhlvsa tmsgvttclr
      241 fpgqlnadlr klavnmvpfp rfhffltgfa pltsrgcqey raltvpeltq qmfdaknmma
      301 acdprhgryl tvaavfrgqm smrevddqml niqnkqsshf vewipnsvkt avcdipprgl
      361 kmsatfigns taiqeifrri seqftamfrr kaflhwytge gmdeyefiea esnlndlvse
      421 yqqyqestpe degefeetqa ea
//
LOCUS       XP_077520852             406 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077520852
VERSION     XP_077520852.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664726.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..406
                     /product="tubulin beta-4 chain-like isoform X3"
                     /calculated_mol_wt=45553
     Region          1..406
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..406
                     /gene="LOC144132363"
                     /coded_by="XM_077664726.1:296..1516"
                     /db_xref="GeneID:144132363"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasgqsga
       61 gnnwakghyt egaelvdsvl dvvrketesc dcmqgfqlth slgggtgsgm gtlliskire
      121 eypdrimnty svvpspkvsd tvvepynatl smhqlventd etycidneal ydicfrtlkl
      181 ttptygdlnh lvsatmsgvt tclrfpgqln adlrklavnm vpfprfhffl tgfapltsrg
      241 cqeyraltvp eltqqmfdak nmmaacdprh gryltvaavf rgqmsmrevd dqmlniqnkq
      301 sshfvewipn svktavcdip prglkmsatf ignstaiqei frriseqfta mfrrkaflhw
      361 ytgegmdeye fieaesnlnd lvseyqqyqe stpedegefe etqaea
//
LOCUS       XP_077520864             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077520864
VERSION     XP_077520864.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664738.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=49744
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144132378"
                     /coded_by="XM_077664738.1:266..1597"
                     /db_xref="GeneID:144132378"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasegkyv
       61 prailvdlep gtmdavrsgq fgqlfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaesfdcm qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsmh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prfhffltgf aphtsrgcqe yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgq msmrevddqm lniqnkqssh fvewipnsvk tavcdipprg
      361 lkmsatfign staiqeifrr iseqftamfr rkaflhwytg egmdeyefie aesnlndlvs
      421 eyqqyqestp edegefeetq aea
//
LOCUS       XP_077520878             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077520878
VERSION     XP_077520878.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664752.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=49616
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144132385"
                     /coded_by="XM_077664752.1:295..1626"
                     /db_xref="GeneID:144132385"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy cneasegkyv
       61 prailvdlep gtmdavrsgq fgqlfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaescdcm qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsmh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prfhffltgf apltsrgcqe yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgq msmrevddqm lniqnkqssh fvewipnsvk tavcdipprg
      361 lkmsatfign staiqeifrr iseqftamfr rkaflhwytg egmdeyefie aesnlndlvs
      421 eyqqyqestp edegefeetq aea
//
LOCUS       XP_077520893             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077520893
VERSION     XP_077520893.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664767.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like isoform X1"
                     /calculated_mol_wt=49676
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144132388"
                     /coded_by="XM_077664767.1:291..1622"
                     /db_xref="GeneID:144132388"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasegkyv
       61 prailvdlep gtmdavrsgq fgqlfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaescdcm qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsmh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prfhffltgf apltsrgcqe yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgq msmrevddqm lniqnkqssh fvewipnsvk tavcdipprg
      361 lkmsatfign staiqeifrr iseqftamfr rkaflhwytg egmdeyefie aesnlndlvs
      421 eyqqyqestp edegefeetq aea
//
LOCUS       XP_077520898             442 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077520898
VERSION     XP_077520898.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664772.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..442
                     /product="tubulin beta-4 chain-like isoform X2"
                     /calculated_mol_wt=49547
     Region          1..442
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..442
                     /gene="LOC144132388"
                     /coded_by="XM_077664772.1:293..1621"
                     /db_xref="GeneID:144132388"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasgkyvp
       61 railvdlepg tmdavrsgqf gqlfrpdnfv fgqsgagnnw akghytegae lvdsvldvvr
      121 keaescdcmq gfqlthslgg gtgsgmgtll iskireeypd rimntysvvp spkvsdtvve
      181 pynatlsmhq lventdetyc idnealydic frtlklttpt ygdlnhlvsa tmsgvttclr
      241 fpgqlnadlr klavnmvpfp rfhffltgfa pltsrgcqey raltvpeltq qmfdaknmma
      301 acdprhgryl tvaavfrgqm smrevddqml niqnkqsshf vewipnsvkt avcdipprgl
      361 kmsatfigns taiqeifrri seqftamfrr kaflhwytge gmdeyefiea esnlndlvse
      421 yqqyqestpe degefeetqa ea
//
LOCUS       XP_077520903             406 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077520903
VERSION     XP_077520903.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664777.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..406
                     /product="tubulin beta-4 chain-like isoform X3"
                     /calculated_mol_wt=45523
     Region          1..406
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..406
                     /gene="LOC144132388"
                     /coded_by="XM_077664777.1:289..1509"
                     /db_xref="GeneID:144132388"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasgqsga
       61 gnnwakghyt egaelvdsvl dvvrkeaesc dcmqgfqlth slgggtgsgm gtlliskire
      121 eypdrimnty svvpspkvsd tvvepynatl smhqlventd etycidneal ydicfrtlkl
      181 ttptygdlnh lvsatmsgvt tclrfpgqln adlrklavnm vpfprfhffl tgfapltsrg
      241 cqeyraltvp eltqqmfdak nmmaacdprh gryltvaavf rgqmsmrevd dqmlniqnkq
      301 sshfvewipn svktavcdip prglkmsatf ignstaiqei frriseqfta mfrrkaflhw
      361 ytgegmdeye fieaesnlnd lvseyqqyqe stpedegefe etqaea
//
LOCUS       XP_077520915             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077520915
VERSION     XP_077520915.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664789.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like isoform X1"
                     /calculated_mol_wt=49684
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144132401"
                     /coded_by="XM_077664789.1:300..1631"
                     /db_xref="GeneID:144132401"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdeygi dpsgayhgds dlqlerinvy yneasegkyv
       61 prailvdlep gtmdavrsgq fgqlfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaescdcm qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsmh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prfhffltgf apltsrgcqe yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgq ismrevddqm lniqnkqssh fvewipnsvk tavcdipprg
      361 lkmsatfign staiqeifrr iseqftamfr rkaflhwytg egmdeyefie aesnlndlvs
      421 eyqqyqestp edegefeetq aea
//
LOCUS       XP_077520923             442 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077520923
VERSION     XP_077520923.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664797.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..442
                     /product="tubulin beta-4 chain-like isoform X2"
                     /calculated_mol_wt=49555
     Region          1..442
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..442
                     /gene="LOC144132401"
                     /coded_by="XM_077664797.1:301..1629"
                     /db_xref="GeneID:144132401"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdeygi dpsgayhgds dlqlerinvy yneasgkyvp
       61 railvdlepg tmdavrsgqf gqlfrpdnfv fgqsgagnnw akghytegae lvdsvldvvr
      121 keaescdcmq gfqlthslgg gtgsgmgtll iskireeypd rimntysvvp spkvsdtvve
      181 pynatlsmhq lventdetyc idnealydic frtlklttpt ygdlnhlvsa tmsgvttclr
      241 fpgqlnadlr klavnmvpfp rfhffltgfa pltsrgcqey raltvpeltq qmfdaknmma
      301 acdprhgryl tvaavfrgqi smrevddqml niqnkqsshf vewipnsvkt avcdipprgl
      361 kmsatfigns taiqeifrri seqftamfrr kaflhwytge gmdeyefiea esnlndlvse
      421 yqqyqestpe degefeetqa ea
//
LOCUS       XP_077520932             406 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077520932
VERSION     XP_077520932.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664806.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..406
                     /product="tubulin beta-4 chain-like isoform X3"
                     /calculated_mol_wt=45531
     Region          1..406
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..406
                     /gene="LOC144132401"
                     /coded_by="XM_077664806.1:299..1519"
                     /db_xref="GeneID:144132401"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdeygi dpsgayhgds dlqlerinvy yneasgqsga
       61 gnnwakghyt egaelvdsvl dvvrkeaesc dcmqgfqlth slgggtgsgm gtlliskire
      121 eypdrimnty svvpspkvsd tvvepynatl smhqlventd etycidneal ydicfrtlkl
      181 ttptygdlnh lvsatmsgvt tclrfpgqln adlrklavnm vpfprfhffl tgfapltsrg
      241 cqeyraltvp eltqqmfdak nmmaacdprh gryltvaavf rgqismrevd dqmlniqnkq
      301 sshfvewipn svktavcdip prglkmsatf ignstaiqei frriseqfta mfrrkaflhw
      361 ytgegmdeye fieaesnlnd lvseyqqyqe stpedegefe etqaea
//
LOCUS       XP_077520939             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077520939
VERSION     XP_077520939.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664813.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like isoform X1"
                     /calculated_mol_wt=49676
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144132413"
                     /coded_by="XM_077664813.1:291..1622"
                     /db_xref="GeneID:144132413"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasegkyv
       61 prailvdlep gtmdavrsgq fgqlfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaescdcm qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsmh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prfhffltgf apltsrgcqe yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgq msmrevddqm lniqnkqssh fvewipnsvk tavcdipprg
      361 lkmsatfign staiqeifrr iseqftamfr rkaflhwytg egmdeyefie aesnlndlvs
      421 eyqqyqestp edegefeetq aea
//
LOCUS       XP_077520948             442 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077520948
VERSION     XP_077520948.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664822.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..442
                     /product="tubulin beta-4 chain-like isoform X2"
                     /calculated_mol_wt=49547
     Region          1..442
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..442
                     /gene="LOC144132413"
                     /coded_by="XM_077664822.1:293..1621"
                     /db_xref="GeneID:144132413"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasgkyvp
       61 railvdlepg tmdavrsgqf gqlfrpdnfv fgqsgagnnw akghytegae lvdsvldvvr
      121 keaescdcmq gfqlthslgg gtgsgmgtll iskireeypd rimntysvvp spkvsdtvve
      181 pynatlsmhq lventdetyc idnealydic frtlklttpt ygdlnhlvsa tmsgvttclr
      241 fpgqlnadlr klavnmvpfp rfhffltgfa pltsrgcqey raltvpeltq qmfdaknmma
      301 acdprhgryl tvaavfrgqm smrevddqml niqnkqsshf vewipnsvkt avcdipprgl
      361 kmsatfigns taiqeifrri seqftamfrr kaflhwytge gmdeyefiea esnlndlvse
      421 yqqyqestpe degefeetqa ea
//
LOCUS       XP_077520957             406 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077520957
VERSION     XP_077520957.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664831.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..406
                     /product="tubulin beta-4 chain-like isoform X3"
                     /calculated_mol_wt=45523
     Region          1..406
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..406
                     /gene="LOC144132413"
                     /coded_by="XM_077664831.1:289..1509"
                     /db_xref="GeneID:144132413"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasgqsga
       61 gnnwakghyt egaelvdsvl dvvrkeaesc dcmqgfqlth slgggtgsgm gtlliskire
      121 eypdrimnty svvpspkvsd tvvepynatl smhqlventd etycidneal ydicfrtlkl
      181 ttptygdlnh lvsatmsgvt tclrfpgqln adlrklavnm vpfprfhffl tgfapltsrg
      241 cqeyraltvp eltqqmfdak nmmaacdprh gryltvaavf rgqmsmrevd dqmlniqnkq
      301 sshfvewipn svktavcdip prglkmsatf ignstaiqei frriseqfta mfrrkaflhw
      361 ytgegmdeye fieaesnlnd lvseyqqyqe stpedegefe etqaea
//
LOCUS       XP_077520965             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077520965
VERSION     XP_077520965.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664839.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like isoform X1"
                     /calculated_mol_wt=49698
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144132430"
                     /coded_by="XM_077664839.1:262..1593"
                     /db_xref="GeneID:144132430"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasegkyv
       61 prailvdlep gtmdavrsgq fgqlfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaescdcm qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsmh qlventdety cihnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prfhffltgf apltsrgcqe yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgq msmrevddqm lniqnkqssh fvewipnsvk tavcdipprg
      361 lkmsatfign staiqeifrr iseqftamfr rkaflhwytg egmdeyefie aesnlndlvs
      421 eyqqyqestp edegefeetq aea
//
LOCUS       XP_077520971             442 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077520971
VERSION     XP_077520971.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664845.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..442
                     /product="tubulin beta-4 chain-like isoform X2"
                     /calculated_mol_wt=49569
     Region          1..442
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..442
                     /gene="LOC144132430"
                     /coded_by="XM_077664845.1:263..1591"
                     /db_xref="GeneID:144132430"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasgkyvp
       61 railvdlepg tmdavrsgqf gqlfrpdnfv fgqsgagnnw akghytegae lvdsvldvvr
      121 keaescdcmq gfqlthslgg gtgsgmgtll iskireeypd rimntysvvp spkvsdtvve
      181 pynatlsmhq lventdetyc ihnealydic frtlklttpt ygdlnhlvsa tmsgvttclr
      241 fpgqlnadlr klavnmvpfp rfhffltgfa pltsrgcqey raltvpeltq qmfdaknmma
      301 acdprhgryl tvaavfrgqm smrevddqml niqnkqsshf vewipnsvkt avcdipprgl
      361 kmsatfigns taiqeifrri seqftamfrr kaflhwytge gmdeyefiea esnlndlvse
      421 yqqyqestpe degefeetqa ea
//
LOCUS       XP_077520979             406 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077520979
VERSION     XP_077520979.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664853.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..406
                     /product="tubulin beta-4 chain-like isoform X3"
                     /calculated_mol_wt=45545
     Region          1..406
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..406
                     /gene="LOC144132430"
                     /coded_by="XM_077664853.1:261..1481"
                     /db_xref="GeneID:144132430"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasgqsga
       61 gnnwakghyt egaelvdsvl dvvrkeaesc dcmqgfqlth slgggtgsgm gtlliskire
      121 eypdrimnty svvpspkvsd tvvepynatl smhqlventd etycihneal ydicfrtlkl
      181 ttptygdlnh lvsatmsgvt tclrfpgqln adlrklavnm vpfprfhffl tgfapltsrg
      241 cqeyraltvp eltqqmfdak nmmaacdprh gryltvaavf rgqmsmrevd dqmlniqnkq
      301 sshfvewipn svktavcdip prglkmsatf ignstaiqei frriseqfta mfrrkaflhw
      361 ytgegmdeye fieaesnlnd lvseyqqyqe stpedegefe etqaea
//
LOCUS       XP_077520987             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077520987
VERSION     XP_077520987.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664861.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like isoform X1"
                     /calculated_mol_wt=49676
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144132446"
                     /coded_by="XM_077664861.1:262..1593"
                     /db_xref="GeneID:144132446"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasegkyv
       61 prailvdlep gtmdavrsgq fgqlfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaescdcm qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsmh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prfhffltgf apltsrgcqe yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgq msmrevddqm lniqnkqssh fvewipnsvk tavcdipprg
      361 lkmsatfign staiqeifrr iseqftamfr rkaflhwytg egmdeyefie aesnlndlvs
      421 eyqqyqestp edegefeetq aea
//
LOCUS       XP_077520995             442 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077520995
VERSION     XP_077520995.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664869.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..442
                     /product="tubulin beta-4 chain-like isoform X2"
                     /calculated_mol_wt=49547
     Region          1..442
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..442
                     /gene="LOC144132446"
                     /coded_by="XM_077664869.1:264..1592"
                     /db_xref="GeneID:144132446"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasgkyvp
       61 railvdlepg tmdavrsgqf gqlfrpdnfv fgqsgagnnw akghytegae lvdsvldvvr
      121 keaescdcmq gfqlthslgg gtgsgmgtll iskireeypd rimntysvvp spkvsdtvve
      181 pynatlsmhq lventdetyc idnealydic frtlklttpt ygdlnhlvsa tmsgvttclr
      241 fpgqlnadlr klavnmvpfp rfhffltgfa pltsrgcqey raltvpeltq qmfdaknmma
      301 acdprhgryl tvaavfrgqm smrevddqml niqnkqsshf vewipnsvkt avcdipprgl
      361 kmsatfigns taiqeifrri seqftamfrr kaflhwytge gmdeyefiea esnlndlvse
      421 yqqyqestpe degefeetqa ea
//
LOCUS       XP_077521004             406 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077521004
VERSION     XP_077521004.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664878.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..406
                     /product="tubulin beta-4 chain-like isoform X3"
                     /calculated_mol_wt=45523
     Region          1..406
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..406
                     /gene="LOC144132446"
                     /coded_by="XM_077664878.1:261..1481"
                     /db_xref="GeneID:144132446"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasgqsga
       61 gnnwakghyt egaelvdsvl dvvrkeaesc dcmqgfqlth slgggtgsgm gtlliskire
      121 eypdrimnty svvpspkvsd tvvepynatl smhqlventd etycidneal ydicfrtlkl
      181 ttptygdlnh lvsatmsgvt tclrfpgqln adlrklavnm vpfprfhffl tgfapltsrg
      241 cqeyraltvp eltqqmfdak nmmaacdprh gryltvaavf rgqmsmrevd dqmlniqnkq
      301 sshfvewipn svktavcdip prglkmsatf ignstaiqei frriseqfta mfrrkaflhw
      361 ytgegmdeye fieaesnlnd lvseyqqyqe stpedegefe etqaea
//
LOCUS       XP_077521019             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077521019
VERSION     XP_077521019.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664893.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like isoform X1"
                     /calculated_mol_wt=49676
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144132463"
                     /coded_by="XM_077664893.1:257..1588"
                     /db_xref="GeneID:144132463"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasegkyv
       61 prailvdlep gtmdavrsgq fgqlfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaescdcm qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsmh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prfhffltgf apltsrgcqe yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgq msmrevddqm lniqnkqssh fvewipnsvk tavcdipprg
      361 lkmsatfign staiqeifrr iseqftamfr rkaflhwytg egmdeyefie aesnlndlvs
      421 eyqqyqestp edegefeetq aea
//
LOCUS       XP_077521027             442 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077521027
VERSION     XP_077521027.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664901.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..442
                     /product="tubulin beta-4 chain-like isoform X2"
                     /calculated_mol_wt=49547
     Region          1..442
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..442
                     /gene="LOC144132463"
                     /coded_by="XM_077664901.1:259..1587"
                     /db_xref="GeneID:144132463"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasgkyvp
       61 railvdlepg tmdavrsgqf gqlfrpdnfv fgqsgagnnw akghytegae lvdsvldvvr
      121 keaescdcmq gfqlthslgg gtgsgmgtll iskireeypd rimntysvvp spkvsdtvve
      181 pynatlsmhq lventdetyc idnealydic frtlklttpt ygdlnhlvsa tmsgvttclr
      241 fpgqlnadlr klavnmvpfp rfhffltgfa pltsrgcqey raltvpeltq qmfdaknmma
      301 acdprhgryl tvaavfrgqm smrevddqml niqnkqsshf vewipnsvkt avcdipprgl
      361 kmsatfigns taiqeifrri seqftamfrr kaflhwytge gmdeyefiea esnlndlvse
      421 yqqyqestpe degefeetqa ea
//
LOCUS       XP_077521036             406 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077521036
VERSION     XP_077521036.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664910.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..406
                     /product="tubulin beta-4 chain-like isoform X3"
                     /calculated_mol_wt=45523
     Region          1..406
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..406
                     /gene="LOC144132463"
                     /coded_by="XM_077664910.1:254..1474"
                     /db_xref="GeneID:144132463"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasgqsga
       61 gnnwakghyt egaelvdsvl dvvrkeaesc dcmqgfqlth slgggtgsgm gtlliskire
      121 eypdrimnty svvpspkvsd tvvepynatl smhqlventd etycidneal ydicfrtlkl
      181 ttptygdlnh lvsatmsgvt tclrfpgqln adlrklavnm vpfprfhffl tgfapltsrg
      241 cqeyraltvp eltqqmfdak nmmaacdprh gryltvaavf rgqmsmrevd dqmlniqnkq
      301 sshfvewipn svktavcdip prglkmsatf ignstaiqei frriseqfta mfrrkaflhw
      361 ytgegmdeye fieaesnlnd lvseyqqyqe stpedegefe etqaea
//
LOCUS       XP_077521047             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077521047
VERSION     XP_077521047.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664921.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like isoform X1"
                     /calculated_mol_wt=49649
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144132477"
                     /coded_by="XM_077664921.1:123..1454"
                     /db_xref="GeneID:144132477"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasegkyv
       61 prailvdlep gtmdavrsgq fgqlfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaescdcm qgfqlthslg ggtgsgmgtl liskireeyp drimstysvv pspkvsdtvv
      181 epynatlsmh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prfhffltgf apltsrgcqe yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgq msmrevddqm lniqnkqssh fvewipnsvk tavcdipprg
      361 lkmsatfign staiqeifrr iseqftamfr rkaflhwytg egmdeyefie aesnlndlvs
      421 eyqqyqestp edegefeetq aea
//
LOCUS       XP_077521056             442 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077521056
VERSION     XP_077521056.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664930.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..442
                     /product="tubulin beta-4 chain-like isoform X2"
                     /calculated_mol_wt=49520
     Region          1..442
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..442
                     /gene="LOC144132477"
                     /coded_by="XM_077664930.1:123..1451"
                     /db_xref="GeneID:144132477"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasgkyvp
       61 railvdlepg tmdavrsgqf gqlfrpdnfv fgqsgagnnw akghytegae lvdsvldvvr
      121 keaescdcmq gfqlthslgg gtgsgmgtll iskireeypd rimstysvvp spkvsdtvve
      181 pynatlsmhq lventdetyc idnealydic frtlklttpt ygdlnhlvsa tmsgvttclr
      241 fpgqlnadlr klavnmvpfp rfhffltgfa pltsrgcqey raltvpeltq qmfdaknmma
      301 acdprhgryl tvaavfrgqm smrevddqml niqnkqsshf vewipnsvkt avcdipprgl
      361 kmsatfigns taiqeifrri seqftamfrr kaflhwytge gmdeyefiea esnlndlvse
      421 yqqyqestpe degefeetqa ea
//
LOCUS       XP_077521063             406 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077521063
VERSION     XP_077521063.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664937.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..406
                     /product="tubulin beta-4 chain-like isoform X3"
                     /calculated_mol_wt=45496
     Region          1..406
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..406
                     /gene="LOC144132477"
                     /coded_by="XM_077664937.1:122..1342"
                     /db_xref="GeneID:144132477"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasgqsga
       61 gnnwakghyt egaelvdsvl dvvrkeaesc dcmqgfqlth slgggtgsgm gtlliskire
      121 eypdrimsty svvpspkvsd tvvepynatl smhqlventd etycidneal ydicfrtlkl
      181 ttptygdlnh lvsatmsgvt tclrfpgqln adlrklavnm vpfprfhffl tgfapltsrg
      241 cqeyraltvp eltqqmfdak nmmaacdprh gryltvaavf rgqmsmrevd dqmlniqnkq
      301 sshfvewipn svktavcdip prglkmsatf ignstaiqei frriseqfta mfrrkaflhw
      361 ytgegmdeye fieaesnlnd lvseyqqyqe stpedegefe etqaea
//
LOCUS       XP_077521076             214 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132494 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077521076
VERSION     XP_077521076.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664950.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..214
                     /product="uncharacterized protein LOC144132494 isoform X1"
                     /calculated_mol_wt=24575
     CDS             1..214
                     /gene="LOC144132494"
                     /coded_by="XM_077664950.1:78..722"
                     /db_xref="GeneID:144132494"
ORIGIN      
        1 mayvrfrwsq elqsssglmn rpraaapgig prfgqggaiy gpllrrphph pvedvlgelw
       61 plindfcgci neyldkqhpy rfqrrpgytp ewstvwevyk hvslaqeymg nllkrsgdep
      121 gtlmdlaeaf gneleklskv lrrkaglpea ewnltasnvl dhivnprere vllrrhqeiv
      181 tlhdslvyca mellpqphrr qtpkadphrr qtpk
//
LOCUS       XP_077521081             206 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132494 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077521081
VERSION     XP_077521081.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664955.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..206
                     /product="uncharacterized protein LOC144132494 isoform X2"
                     /calculated_mol_wt=23560
     CDS             1..206
                     /gene="LOC144132494"
                     /coded_by="XM_077664955.1:78..698"
                     /db_xref="GeneID:144132494"
ORIGIN      
        1 mayvrfssgl mnrpraaapg igprfgqgga iygpllrrph phpvedvlge lwplindfcg
       61 cineyldkqh pyrfqrrpgy tpewstvwev ykhvslaqey mgnllkrsgd epgtlmdlae
      121 afgnelekls kvlrrkaglp eaewnltasn vldhivnpre revllrrhqe ivtlhdslvy
      181 camellpqph rrqtpkadph rrqtpk
//
LOCUS       XP_077521089             202 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132494 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077521089
VERSION     XP_077521089.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664963.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..202
                     /product="uncharacterized protein LOC144132494 isoform X3"
                     /calculated_mol_wt=23063
     CDS             1..202
                     /gene="LOC144132494"
                     /coded_by="XM_077664963.1:70..678"
                     /db_xref="GeneID:144132494"
ORIGIN      
        1 mlnsglmnrp raaapgigpr fgqggaiygp llrrphphpv edvlgelwpl indfcgcine
       61 yldkqhpyrf qrrpgytpew stvwevykhv slaqeymgnl lkrsgdepgt lmdlaeafgn
      121 eleklskvlr rkaglpeaew nltasnvldh ivnprerevl lrrhqeivtl hdslvycame
      181 llpqphrrqt pkadphrrqt pk
//
LOCUS       XP_077521109             492 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132516 [Amblyomma americanum].
ACCESSION   XP_077521109
VERSION     XP_077521109.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664983.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..492
                     /product="uncharacterized protein LOC144132516"
                     /calculated_mol_wt=53294
     CDS             1..492
                     /gene="LOC144132516"
                     /coded_by="XM_077664983.1:278..1756"
                     /db_xref="GeneID:144132516"
ORIGIN      
        1 msvvaespkv tvskqtfqys sqileisres apekatlptr sgrssqtssn egklkvqeke
       61 keatvgsnqk psskpskakr tpaskrptaa dvttelekag msasknlafd ntdvtaaafg
      121 ratraarpky vetdkrtdvk mprqeakrtv splmtleaeq rpasnkelge tpkqeaadtk
      181 kssrkilqak tarstrkmne geevpelgta gvspskcssa tnvhvldepa rltrgcrtrg
      241 srkdiveapk ekkvdqpqst katraehtqg srkvckeget lqdvtgsslv masttqrkdv
      301 tfdkhlqgea sptkevsvtr ntrakrkvdl keesiaekht taedligtva eksgghqqrv
      361 asgeyapqen dvqcsnsakq vinpkitrdk evvddapasp nvevatvepa saggkalatt
      421 mepkrskrtm aavkvdvaqt aneatprktr grraealkta lewaddkpga srrtakdrse
      481 eepppkrtrk mv
//
LOCUS       XP_077521120             174 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132519 [Amblyomma americanum].
ACCESSION   XP_077521120
VERSION     XP_077521120.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077664994.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..174
                     /product="uncharacterized protein LOC144132519"
                     /calculated_mol_wt=19673
     Region          <8..>88
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..174
                     /gene="LOC144132519"
                     /coded_by="XM_077664994.1:1..525"
                     /db_xref="GeneID:144132519"
ORIGIN      
        1 mmyskawilq ciimqmkspr lyehmrrqni ltlpskvtlq rylksyrtgf gfnenvlnil
       61 kqktstmdif krhgglivde mklsenlcvs ssghiqgfvd rskftprsem hnvadhemvi
      121 mfvpfagkwt hilacfatrg ntkidlltkv mleavilaen aglfvdvias dggt
//
LOCUS       XP_077521133             214 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132527 [Amblyomma americanum].
ACCESSION   XP_077521133
VERSION     XP_077521133.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665007.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..214
                     /product="uncharacterized protein LOC144132527"
                     /calculated_mol_wt=24619
     CDS             1..214
                     /gene="LOC144132527"
                     /coded_by="XM_077665007.1:77..721"
                     /db_xref="GeneID:144132527"
ORIGIN      
        1 mayvrfrwsq elqsssglmn rpraaapgig prfgqggaiy gpllrrphph pvedvlgelw
       61 plindfcgci neyldkqhpy rfqrrpgytp ewstvwevyk hvslaqeymg nllkrsedep
      121 gtlmdlaeaf gneleklskv lrrkaglpea ewnltasnvl dhivnprere vllqrhqeiv
      181 tlhdslvyca mellpqphrr qtpkadphrr qtpk
//
LOCUS       XP_077521153             188 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132539 [Amblyomma americanum].
ACCESSION   XP_077521153
VERSION     XP_077521153.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665027.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..188
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..188
                     /product="uncharacterized protein LOC144132539"
                     /calculated_mol_wt=20081
     CDS             1..188
                     /gene="LOC144132539"
                     /coded_by="XM_077665027.1:164..730"
                     /db_xref="GeneID:144132539"
ORIGIN      
        1 mlpdyweapg sgrqdlledp asweeqrdee pcdgsgivar adtaladver eafeagweth
       61 resppsssad alpdhweapa ssgrdlreep ssleeqheek pydrsgmvah agctsdtawa
      121 hvksevksgd aslvgksmhg lgevhsqgcp agsvhvegaa qgtalrpvsr sqggqhrkwr
      181 lhlagapm
//
LOCUS       XP_077521187            1056 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132567 [Amblyomma americanum].
ACCESSION   XP_077521187
VERSION     XP_077521187.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665061.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1056
                     /product="uncharacterized protein LOC144132567"
                     /calculated_mol_wt=120606
     Region          92..652
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1056
                     /gene="LOC144132567"
                     /coded_by="XM_077665061.1:13..3183"
                     /db_xref="GeneID:144132567"
ORIGIN      
        1 mdaqscretl lelsaraeec praleealfe iyfslsqgkv fwfrsalehl yfrywahrqr
       61 idvftgsvia qelpshmvkv rkviltptrr vymppqlvck srllqhcdpd yalrivvrdd
      121 dcklisfslg ackddflsav ikpplssglk iggrrfmflg cstsqlrshg vwfyahdnqg
      181 rtaeqirdgi gdlstivsvp kfmarlgqaf sqslglltvp sqntsvepdi rhaapgrssd
      241 gshreyifsd gigrishgll rkvhhalele egeepcavqi ryggckgmll ldptlsgcri
      301 vfresmrkfh sdhsdlyvlk tskprvlyln rpmitileqs gikaevflml qnkildsfid
      361 smmdpheaah vlrsycawrl pykelagvgi dltvepffra lvravnkkvl kelrtkaril
      421 vppnygrtmf gvldetgtle ygqvfvqysn dmlrykvndp atilegdviv tknpcmnpgd
      481 irkleavnvp qlhhvrdciv fpqkgerphp demagsdldg deysvlwyed lifnnncnpm
      541 hyhsdppker kasigiqdmv dffcqyikgd kiglianahl vwadildsgi nshrcrelar
      601 kcavnldfak cgdlkgfqns ekppmypdfm ekldtkntyc srkvlgqlyr nckkvelsse
      661 clevveeslp dprlllegre qflkeatsay kryakkiral lksyrietes eafsgavskl
      721 skymkendpt dmsmvlesqv ehvvrrtree ffseqleeah enlkasawyq vtyelqsseg
      781 giqsfpwvvc dvlmrivvnt ssclpvpasr nsfcqrlgal llglphpggg dqdgtqhgdt
      841 qvltnllrlm ydwidssref lfvknteelg vyksimreac fkvsrsisrd mpphklvilc
      901 lrfacawclk ifqggsdgev iskecrhryr lghlalitln rlsmsgnlay lrrapegcps
      961 teliriyinr edeeffeilr ryediikkim mdwsgvediq cdlktdrmde wflqlmvtgs
     1021 rwalerlkei vvypsfrevl llaferekna iggtle
//
LOCUS       XP_077521210             161 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132588 [Amblyomma americanum].
ACCESSION   XP_077521210
VERSION     XP_077521210.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665084.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..161
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..161
                     /product="uncharacterized protein LOC144132588"
                     /calculated_mol_wt=17572
     CDS             1..161
                     /gene="LOC144132588"
                     /coded_by="XM_077665084.1:13..498"
                     /db_xref="GeneID:144132588"
ORIGIN      
        1 mpsallwtfl ptylvqlwst rsaraaanqf rqlmqklfkm hspslkhqvh flgglwllhl
       61 dasccavass shqgaspsqr ksfslwlslf rpsccrqlrt ssgswsnril chravttvas
      121 trdprggcqp gtggielvsq gvqqapssfq gdrpaccrsg r
//
LOCUS       XP_077521234             186 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132596 [Amblyomma americanum].
ACCESSION   XP_077521234
VERSION     XP_077521234.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665108.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..186
                     /product="uncharacterized protein LOC144132596"
                     /calculated_mol_wt=19548
     CDS             1..186
                     /gene="LOC144132596"
                     /coded_by="XM_077665108.1:13..573"
                     /db_xref="GeneID:144132596"
ORIGIN      
        1 maalgragll llasavvvgc gqasgtddga apigvgpaga erqhrgfqql rlstargfgk
       61 rgalpaiaal lghhvalhrl paaqqplmkk gfrnmkista rgfgkradpd ttflldnddf
      121 dpmdykskrd irrislstar gfgkrlssgs sdesyqpaaq paatwlaeev akadlnddga
      181 vyqdtf
//
LOCUS       XP_077521273             186 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132611 [Amblyomma americanum].
ACCESSION   XP_077521273
VERSION     XP_077521273.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665147.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..186
                     /product="uncharacterized protein LOC144132611"
                     /calculated_mol_wt=19512
     CDS             1..186
                     /gene="LOC144132611"
                     /coded_by="XM_077665147.1:13..573"
                     /db_xref="GeneID:144132611"
ORIGIN      
        1 maalgragll llasavvvgc gqasgtddda apigvgpaga erqhrgfqql rlstargfgk
       61 rgalpaiaal lghhvalhrl paaqqplmkk gfrnmkista rglgkradpd ttslldnddf
      121 dpmdykskrd irrislstar gfgkrlssgs sdesyqpaaq paatwlaeev akadlnddga
      181 vyqdtf
//
LOCUS       XP_077521291            1056 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132629 [Amblyomma americanum].
ACCESSION   XP_077521291
VERSION     XP_077521291.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665165.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1056
                     /product="uncharacterized protein LOC144132629"
                     /calculated_mol_wt=120580
     Region          92..652
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1056
                     /gene="LOC144132629"
                     /coded_by="XM_077665165.1:13..3183"
                     /db_xref="GeneID:144132629"
ORIGIN      
        1 mdaqscretl lelsaraeec praleealfe iyfslsqgkv fwfrsalehl yfryrahrqr
       61 idvftgsvia qelpshmvkv rkviltptrr vymppqlvck srllqhcdpd yalrivvrdd
      121 dcklisfslg ackddflsav ikpplssglk iggrrfmflg cstsqlrshg vwfyahdnqg
      181 rtaeqirdgi gdlstivsvp kfmarlgqaf sqslglltvp sqntsvepdi rhaapgrssd
      241 ashreyifsd gigrishgll rkvhhalele egeepcavqi ryggckgmll ldptlsgcri
      301 vfresmrkfh sdhsdlyvlk tskprvlyln rpmitileqs gikaevflml qnkildsfid
      361 smmdpheaah vlrsycalrl pykelagvgi dltvepffra lvravnkkvl kelrtkaril
      421 vppnygrtmf gvldetgtle ygqvfvqysn dmlrykvndp atilegdviv tknpcmnpgd
      481 irkleavnvp qlhhvrdciv fpqkgerphp demagsdldg deysvlwyed lifnnncnpm
      541 hyhsdppker kasigvqdmv dffcqyikgd kiglianahl vwadildsgi nshrcrelar
      601 kcavnldfak cgdlkgfqns eqppmypdfm ekldtkntyc srkvlgqlyr nckkvelste
      661 clevveeslp dprlllegre qflkeatsay kryakkiral lksyrietes eafsgavskl
      721 skymkendpt dmamvlesqv ehvvrrtree ffseqldeah eklkasawyq vtyelqsseg
      781 giqsfpwvvy dvlmrivvnt ssclpvpasr nsfcqrlgal llglphpggg dqdgtqhgdt
      841 qvltnllrlm ydwidssref lfvknteelg vyksimreac fkvsrsisrd mpphklvilc
      901 lrfacawclk ifqggsdgev iskecrrryr lghlalitln rlsmsgnlay lrrapegcps
      961 teliriyinr edeeffeilr ryediikkim mdwsgvediq cdlktdrmde wflqlmvtgs
     1021 rwalerlkei vvypsfrevl llaferekna iggtle
//
LOCUS       XP_077521306             161 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132631 [Amblyomma americanum].
ACCESSION   XP_077521306
VERSION     XP_077521306.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665180.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..161
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..161
                     /product="uncharacterized protein LOC144132631"
                     /calculated_mol_wt=17598
     CDS             1..161
                     /gene="LOC144132631"
                     /coded_by="XM_077665180.1:309..794"
                     /db_xref="GeneID:144132631"
ORIGIN      
        1 mpsallwtfl ptylvqlwst rsaraaanqf rqlmqklfkm hspslkhqvh flgglwllhl
       61 dasccavass shqgaspsqr ksfslwlllf rpsccrqlrt ssgswsnril chravttvas
      121 trdprggcqp gtggielvsq gvqqapssfq gdrpaccrsg r
//
LOCUS       XP_077521375            1056 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC144132663
            [Amblyomma americanum].
ACCESSION   XP_077521375
VERSION     XP_077521375.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665249.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1056
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC144132663"
                     /calculated_mol_wt=120436
     Region          92..652
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1056
                     /gene="LOC144132663"
                     /coded_by="XM_077665249.1:13..3183"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 1 base in 1 codon"
                     /db_xref="GeneID:144132663"
ORIGIN      
        1 mdaqscretl lelsaraeec praleealfe iyfslsqgkv fwfrsalehl yfryrahrqr
       61 idvftgsvia qelpshmvkv rkviltptrr vymppqlvck srllqhcdpd yalrivvrdd
      121 dcklisfslg ackddflsav ikpplssglk iggrrfmflg cstsqlrshg vwfyahdnqg
      181 rtaeqirdgi gdxstivsvp kfmarlgqaf sqslglltvp sqntsvepdi rhaapgrssd
      241 gshreyifsd gigrishgll rkvhhalele egeepcavqi ryggckgmll ldptlsgcri
      301 vfresmrkfh sdhsdlyvlk tskprvlyln rpmitileqs gikaevflml qnkildsfid
      361 smmdpheaah vlrsycalrl pykelagvgi dltvepffra lvravnkkvl kelrtkaril
      421 vppnygrtmf gvldetgtle ygqvfvqysn dmlrykvndp atilegdviv tknpcmnpgd
      481 irkleavnvp qlhhvrdciv fpqkgerphp demagsdldg deysvlwyed lifnnncnpm
      541 hyhsdppker kasigvqdmv dffcqyikgd kiglianahl vwadildsgi nshrcrelar
      601 kcavnldfak cgdlkgfqns eqppmypdfm ekldtkntys srkvlgqlyr nckkvelste
      661 clevveeslp dprlllegre qflkeatsay kryakkiral lksyrietes eafsgavskl
      721 skymkendpt dmamvlesqv ehvvrrtree ffseqldeah eklkasawyq vtyelqsseg
      781 giqsfpwvvy dvlmrivvnt ssclpvpasr nsfcqrlgal llglphpggg dqdgtqhgdt
      841 qvltnllrlm ydwidssref lfvknteelg vyksimreac fkvsrsisrd mpphklvilc
      901 lrfacawclk ifqggsdgev iskecrrryr lghlalitln rlsmsgnlay lrrapegcps
      961 teliriyinr edeeffeilr ryediikkim mdwsgvediq cdlktdrmde wflqlmvtgs
     1021 rwalerlkei vvypsfrevl llaferekna iggtle
//
LOCUS       XP_077521391             333 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132674 [Amblyomma americanum].
ACCESSION   XP_077521391
VERSION     XP_077521391.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665265.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..333
                     /product="uncharacterized protein LOC144132674"
                     /calculated_mol_wt=38438
     CDS             1..333
                     /gene="LOC144132674"
                     /coded_by="XM_077665265.1:132..1133"
                     /db_xref="GeneID:144132674"
ORIGIN      
        1 mkendptdma mvlesqvehv vrrtreeffs eqldeahekl kasawyqvty elqsseggiq
       61 sfpwvvydvl mrivvntssc lpvpasrnsf cqrlgalllg lphpgggdqd gtqhgdtqvl
      121 tnllrlmydw idssreflfv knteelgvyk simreacfkv srsisrdmpp hklvilclrf
      181 acawclkifq ggsdgevisk ecrrryrlgh lalitlnrls msgnlaylrr apegcpstel
      241 iriyinrede effeilrrye diikkimmdw sgvediqcdl ktdrmdewfl qlmvtgsrwa
      301 lerlkeivvy psfrevllla fereknaigg tle
//
LOCUS       XP_077521420             186 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132703 [Amblyomma americanum].
ACCESSION   XP_077521420
VERSION     XP_077521420.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665294.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..186
                     /product="uncharacterized protein LOC144132703"
                     /calculated_mol_wt=19406
     CDS             1..186
                     /gene="LOC144132703"
                     /coded_by="XM_077665294.1:13..573"
                     /db_xref="GeneID:144132703"
ORIGIN      
        1 maalgragll llasavvvgc gqasgtddga apigvgpaga erqhrgfqql rlstargfgk
       61 rgalpaiaal lghhvalhrl paaqqplikk gfrnmkista rglgkradpd ttslldnddf
      121 dpmdykskrd irrislstar gfgkrlssgs sdvsyqpaaq paatwlaeev akadlnddga
      181 vyqdtf
//
LOCUS       XP_077521468             551 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132730 [Amblyomma americanum].
ACCESSION   XP_077521468
VERSION     XP_077521468.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665342.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..551
                     /product="uncharacterized protein LOC144132730"
                     /calculated_mol_wt=60975
     Region          <25..223
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     CDS             1..551
                     /gene="LOC144132730"
                     /coded_by="XM_077665342.1:60..1715"
                     /db_xref="GeneID:144132730"
ORIGIN      
        1 mgfnlsvada thlaalvarn kglkklalvd lyidaasmee ivasieghdg ldeleltalr
       61 rtadgksiar lleahitklr lgvecdwepf ldalvtnidl vefaivsrst lafslhrlvr
      121 avsisetles leldldtswa idddriweel aaivgqaral rmlritvsnm tnvalellkn
      181 eiarnqclrd lhfhrcqisc hgamellggl rcnstlqlld laelsgrege yrellqfmvd
      241 nrlcdrvsvc ymgcqawllg kaitnngvrf rnfkfccayg pevdivfgvl pclkdtltel
      301 sidcnevmyd tgtqalaklf rdslilenvk ldfpstpdts lvllqglaes rsvssltlsg
      361 wmiggtddvv piafedllrv nssivqltvv qgdlgelkvf lkrlaaglae nhsiaelnif
      421 hgpdclevcd aaikqslrvn rtmaclaadl llgaklsraa ayslgcvisc nsmktilrsh
      481 tslsddvine klvgtlsair sellqverly ksearrykpp hysegqrydf evellsdiek
      541 ylnlwnvsls e
//
LOCUS       XP_077521476             297 aa            linear   INV 25-NOV-2025
DEFINITION  carboxy-terminal domain RNA polymerase II polypeptide A small
            phosphatase 1-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077521476
VERSION     XP_077521476.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665350.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..297
                     /product="carboxy-terminal domain RNA polymerase II
                     polypeptide A small phosphatase 1-like isoform X1"
                     /calculated_mol_wt=32455
     Region          81..240
                     /region_name="HIF-SF_euk"
                     /note="Dullard-like phosphatase domain; TIGR02251"
                     /db_xref="CDD:274055"
     Site            order(87..91,95..96,142..145,149,181,197..198,200..202)
                     /site_type="active"
                     /db_xref="CDD:319823"
     CDS             1..297
                     /gene="LOC144132736"
                     /coded_by="XM_077665350.1:8..901"
                     /db_xref="GeneID:144132736"
ORIGIN      
        1 mdstsiitqv nreeeqltnf ppdrvpsskk prqrgflntl lccfgsnnqg asnpviaeen
       61 gqyspkgkfl lppvryqdmh kicliidlde tlvhssikpi snadfvvpve tggtvhqvyv
      121 lkrpyvdefl qrvgdayecv lftaslakya dpvadqldkw gvfrarlfre scvfhrgnyv
      181 kdlgrlgrdl hrvviidnsp asymfhpgna vpvgswfddm sdtelrdlmp ffdklsrved
      241 vytvlrnsnn aagggggggg spafpapllm ngaalhavva psispdkgph ggavarh
//
LOCUS       XP_077521486             353 aa            linear   INV 25-NOV-2025
DEFINITION  carboxy-terminal domain RNA polymerase II polypeptide A small
            phosphatase 1-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077521486
VERSION     XP_077521486.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665360.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..353
                     /product="carboxy-terminal domain RNA polymerase II
                     polypeptide A small phosphatase 1-like isoform X2"
                     /calculated_mol_wt=38337
     Region          137..296
                     /region_name="HIF-SF_euk"
                     /note="Dullard-like phosphatase domain; TIGR02251"
                     /db_xref="CDD:274055"
     Site            order(143..147,151..152,198..201,205,237,253..254,
                     256..258)
                     /site_type="active"
                     /db_xref="CDD:319823"
     CDS             1..353
                     /gene="LOC144132736"
                     /coded_by="XM_077665360.1:8..1069"
                     /db_xref="GeneID:144132736"
ORIGIN      
        1 mdstsiitqv nreeeqltnf ppdrvpsskk prqrgflntl lccfgsnnqg asnpviaeen
       61 gqyspkasks srqsappcgt stspiaqlqq nqapapvlar eyaaqeytki sssnltvllp
      121 lqgkfllppv ryqdmhkicl iidldetlvh ssikpisnad fvvpvetggt vhqvyvlkrp
      181 yvdeflqrvg dayecvlfta slakyadpva dqldkwgvfr arlfrescvf hrgnyvkdlg
      241 rlgrdlhrvv iidnspasym fhpgnavpvg swfddmsdte lrdlmpffdk lsrvedvytv
      301 lrnsnnaagg ggggggspaf papllmngaa lhavvapsis pdkgphggav arh
//
LOCUS       XP_077521500             177 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132742 [Amblyomma americanum].
ACCESSION   XP_077521500
VERSION     XP_077521500.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665374.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..177
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..177
                     /product="uncharacterized protein LOC144132742"
                     /calculated_mol_wt=18762
     CDS             1..177
                     /gene="LOC144132742"
                     /coded_by="XM_077665374.1:180..713"
                     /db_xref="GeneID:144132742"
ORIGIN      
        1 msrtpkaaag pnqlrdelrq qllttfaank resamgegtq avedscsaas gkplkkqplp
       61 vvsatscgch kyddlhvhvt vlcpcsrptt tdkatqtdma rtdeqqrstp rgvgisvdil
      121 htsnermfsq tcshelaasv pngtpvtprl qgvpnsadis vqafedegnv siedgsk
//
LOCUS       XP_077521512             131 aa            linear   INV 25-NOV-2025
DEFINITION  F-box only protein 41-like [Amblyomma americanum].
ACCESSION   XP_077521512
VERSION     XP_077521512.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665386.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..131
                     /product="F-box only protein 41-like"
                     /calculated_mol_wt=14342
     CDS             1..131
                     /gene="LOC144132748"
                     /coded_by="XM_077665386.1:811..1206"
                     /db_xref="GeneID:144132748"
ORIGIN      
        1 mlgkcwpqlq vacvggrslt lasvccvlrs cvnlvsfeid rgreideasa eamveaglhr
       61 ierlyfthtv ishravvilq gacprmkqts iilhgrdfgc asalndcvas lkvlqrtkpf
      121 dimlrlvltd e
//
LOCUS       XP_077521525             630 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132756 [Amblyomma americanum].
ACCESSION   XP_077521525
VERSION     XP_077521525.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665399.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..630
                     /product="uncharacterized protein LOC144132756"
                     /calculated_mol_wt=69908
     Region          <220..418
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     Region          <322..606
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     CDS             1..630
                     /gene="LOC144132756"
                     /coded_by="XM_077665399.1:496..2388"
                     /db_xref="GeneID:144132756"
ORIGIN      
        1 mpepivpppq vqcrtinaaa iefsrllhet dasvfdspes alkifqrgde lfrgtnddvp
       61 pltapcngdg ertrcwalny qrmlnrmlne rgvelfemhe sallcqtisc tdvpqgtdda
      121 miptvllwll rqhqcirsvi vnasimaphp lkkfwsllll selvksvelr dveensfsih
      181 rvfhehaerl tvlvlmgfnl svadathlaa lvarnkglkk lalvdlyida asmeeivasi
      241 eghdgldele ltalrrtadg ksiarlleah itklrlgvec dwepfldalv tnidlvelai
      301 vsrstlafsl hrlvravsis etlesleldl dtswaidddr iweelaaivg qaralrmlri
      361 tvsnmtnval ellknviarn qclrdlhfhr cqischgame llgglrcnst lqlldlaels
      421 gregeyrell qfmvdnrlcd rvsvcymgcq awllgkaitn ngvrfrnfkf ccaygpeadi
      481 vfgvlpclkd tltelsidcn evmcdtgtqa laklfrdsli lenvkldcps tpdtslvllq
      541 glaesrsvss ltlsgwmigg mddvvpiafe dllrvnssiv qltvvqgdlg elkvflkrla
      601 aglaenhsis elnilhgpdc levcdatikq
//
LOCUS       XP_077521536             200 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132764 [Amblyomma americanum].
ACCESSION   XP_077521536
VERSION     XP_077521536.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665410.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..200
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..200
                     /product="uncharacterized protein LOC144132764"
                     /calculated_mol_wt=21897
     Region          49..89
                     /region_name="F-box"
                     /note="F-box domain; pfam00646"
                     /db_xref="CDD:425796"
     CDS             1..200
                     /gene="LOC144132764"
                     /coded_by="XM_077665410.1:7..609"
                     /db_xref="GeneID:144132764"
ORIGIN      
        1 mtgsydsshg devieratqt kprprrlagk prvkfalnar dtapsksadl aenvletivq
       61 flstedllic rrvcwawkri verpqhwlri dlnhtsvsae lfskmaawcs ateslslqal
      121 eatadankrv pgahlehgle kllmacsktl kvltvvgsdn llthrfaral cgpiqtsqqp
      181 laasrsqgqr esaakspvds
//
LOCUS       XP_077521549             257 aa            linear   INV 25-NOV-2025
DEFINITION  carboxy-terminal domain RNA polymerase II polypeptide A small
            phosphatase 1-like [Amblyomma americanum].
ACCESSION   XP_077521549
VERSION     XP_077521549.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665423.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..257
                     /product="carboxy-terminal domain RNA polymerase II
                     polypeptide A small phosphatase 1-like"
                     /calculated_mol_wt=27824
     Region          41..200
                     /region_name="HIF-SF_euk"
                     /note="Dullard-like phosphatase domain; TIGR02251"
                     /db_xref="CDD:274055"
     Site            order(47..51,55..56,102..105,109,141,157..158,160..162)
                     /site_type="active"
                     /db_xref="CDD:319823"
     CDS             1..257
                     /gene="LOC144132771"
                     /coded_by="XM_077665423.1:53..826"
                     /db_xref="GeneID:144132771"
ORIGIN      
        1 matyssmdly sscptamfvg taptengkfl lppvryqdmh kicliidlde tlvhssikpi
       61 snadfvvpve tggtvhqvyv lkrpyvdefl qrvgdayecv lftaghakya dpvadqldkw
      121 gvfrarlfre scvfhqgnyv kdlgrlgrdl hrvviidnsp asyvfhpgna vpvgswfddm
      181 sdtelrdlmp ffdklsrved vytvlrnsnn aagggggggg spafpapllm ngaalhavva
      241 psispdkgph ggavarh
//
LOCUS       XP_077521577             295 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132792 [Amblyomma americanum].
ACCESSION   XP_077521577
VERSION     XP_077521577.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665451.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..295
                     /product="uncharacterized protein LOC144132792"
                     /calculated_mol_wt=32595
     Region          144..184
                     /region_name="F-box"
                     /note="F-box domain; pfam00646"
                     /db_xref="CDD:425796"
     CDS             1..295
                     /gene="LOC144132792"
                     /coded_by="XM_077665451.1:79..966"
                     /db_xref="GeneID:144132792"
ORIGIN      
        1 mkldrealsy qmyaadtspp hiyprerppa atvyqefcpl tvpkegqhpv htvtdssnvg
       61 srdkveaavq aaeirrhqsn elfnfpkday cpakdmtgsy dsshgdevie ratqtkprpr
      121 rlagkprvkf alnardtaps ksadlaenvl etivqflste dllicrrvcw awkriverpq
      181 hwlridlnht svsaelfskm aawcsatesl slqaleatad ankrvpgahl ehglekllma
      241 csktlkvltv vgsdnllthr faralcgpiq tsqqplaasr sqgqresaak spvds
//
LOCUS       XP_077521589             257 aa            linear   INV 25-NOV-2025
DEFINITION  carboxy-terminal domain RNA polymerase II polypeptide A small
            phosphatase 1-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077521589
VERSION     XP_077521589.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665463.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..257
                     /product="carboxy-terminal domain RNA polymerase II
                     polypeptide A small phosphatase 1-like isoform X1"
                     /calculated_mol_wt=27814
     Region          41..200
                     /region_name="HIF-SF_euk"
                     /note="Dullard-like phosphatase domain; TIGR02251"
                     /db_xref="CDD:274055"
     Site            order(47..51,55..56,102..105,109,141,157..158,160..162)
                     /site_type="active"
                     /db_xref="CDD:319823"
     CDS             1..257
                     /gene="LOC144132799"
                     /coded_by="XM_077665463.1:63..836"
                     /db_xref="GeneID:144132799"
ORIGIN      
        1 matyssmdly sscptamfvg taptengkfl lppvryqdmh kicliidlde tlvhssikpi
       61 snadfvvpve tggtvhqvyv lkrpyvdefl qrvgdayecv lftaglakya dpvadqldkw
      121 gvfrarlfre scvfhqgkyv kdlgrlgrdl hrvviidnsp asyvfhpgna vpvgswfddm
      181 sdtelrdlmp ffdklsrved vytvlrnsnn aagggggggg spafpapllm ngaalhavva
      241 psispdkgph ggavarh
//
LOCUS       XP_077521597             238 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132799 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077521597
VERSION     XP_077521597.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665471.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..238
                     /product="uncharacterized protein LOC144132799 isoform X2"
                     /calculated_mol_wt=25540
     Region          41..>60
                     /region_name="HAD_like"
                     /note="Haloacid Dehalogenase-like Hydrolases; cl21460"
                     /db_xref="CDD:473868"
     CDS             1..238
                     /gene="LOC144132799"
                     /coded_by="XM_077665471.1:63..779"
                     /db_xref="GeneID:144132799"
ORIGIN      
        1 matyssmdly sscptamfvg taptengkfl lppvryqdmh kicliidlde tlvhssikqc
       61 rlrgasgdww hstpgvcpee alrgrifaas rrclrmclih srpckvcrsc ggptgqvgcl
      121 pgavipgvmr lppgkirqgp gpagpgpapg gdhrqlscll rlppgqcsac gvlvrrhvgh
      181 raagpdavlr qtepcrrcvh gaaklqqccr rwrrrrrlts lpgtaaherc sasccggs
//
LOCUS       XP_077521603             159 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132811 [Amblyomma americanum].
ACCESSION   XP_077521603
VERSION     XP_077521603.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665477.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..159
                     /product="uncharacterized protein LOC144132811"
                     /calculated_mol_wt=17519
     CDS             1..159
                     /gene="LOC144132811"
                     /coded_by="XM_077665477.1:41..520"
                     /db_xref="GeneID:144132811"
ORIGIN      
        1 mggrryigke fppmplfqsq vnresytyvf prvaqpvrta lrtgpdrcsq eppngpeahr
       61 rnskagflsf rdhpadfens rvanskanpt gaidlraley antpgkvnks agclpsphtt
      121 klriapraph pvkpedakca pkgavrqaia yvryrrgdr
//
LOCUS       XP_077521612             305 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132820 [Amblyomma americanum].
ACCESSION   XP_077521612
VERSION     XP_077521612.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665486.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..305
                     /product="uncharacterized protein LOC144132820"
                     /calculated_mol_wt=35083
     Region          163..>287
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..305
                     /gene="LOC144132820"
                     /coded_by="XM_077665486.1:34..951"
                     /db_xref="GeneID:144132820"
ORIGIN      
        1 mamfrtllvl kqlqtgvrrh wvrpvwqnra eeseyftamq lmkngdeslf hkyyrmspei
       61 fdmlhsmvde klskqwlcrd pissgerlal tlrylssgmp ipdvamafri gietareaih
      121 ltcqvlwdvl splymkppte sewrdiaagf whqwqfpncl gavdgkhvni krpkksgsly
      181 fnykgtysiv lmavvdsryq frlidvgapg rlsdggifkd spigkrlekg klnlpraaql
      241 pgsaatlphv figdeafqlr pdfmrplpgs rtsneevifn yrlsraryiq hgtnlllraf
      301 lyeff
//
LOCUS       XP_077521628             115 aa            linear   INV 25-NOV-2025
DEFINITION  transcription factor Adf-1-like [Amblyomma americanum].
ACCESSION   XP_077521628
VERSION     XP_077521628.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665502.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..115
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..115
                     /product="transcription factor Adf-1-like"
                     /calculated_mol_wt=13560
     Region          10..109
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     CDS             1..115
                     /gene="LOC144132822"
                     /coded_by="XM_077665502.1:232..579"
                     /db_xref="GeneID:144132822"
ORIGIN      
        1 maamqefner lieqvqeerv lwdmsythyk sqqrrnvawr riaatlgstv pdvkarwknl
       61 rdtfrrlyka rnptprsgap aedddedcfe gddaatkwff ydrllflrdt vtgrp
//
LOCUS       XP_077521640             191 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132830 [Amblyomma americanum].
ACCESSION   XP_077521640
VERSION     XP_077521640.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665514.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..191
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..191
                     /product="uncharacterized protein LOC144132830"
                     /calculated_mol_wt=20912
     CDS             1..191
                     /gene="LOC144132830"
                     /coded_by="XM_077665514.1:1..576"
                     /db_xref="GeneID:144132830"
ORIGIN      
        1 mssapieclv twegrkqvlf vggdrkksdv inaamladsw aslqcdriev ynsrfdtyvd
       61 vpeghtfsdg dkinlvgsss sdeqnlplet gtddgplvla pscsepdkrl ftvqqplyel
      121 ppipldikad tegttlgevs srtknrivsw itahlltidw ypgkfyepaa kalvlkypvl
      181 rdtigtgyvs t
//
LOCUS       XP_077521652             297 aa            linear   INV 25-NOV-2025
DEFINITION  carboxy-terminal domain RNA polymerase II polypeptide A small
            phosphatase 1-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077521652
VERSION     XP_077521652.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665526.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..297
                     /product="carboxy-terminal domain RNA polymerase II
                     polypeptide A small phosphatase 1-like isoform X1"
                     /calculated_mol_wt=32455
     Region          81..240
                     /region_name="HIF-SF_euk"
                     /note="Dullard-like phosphatase domain; TIGR02251"
                     /db_xref="CDD:274055"
     Site            order(87..91,95..96,142..145,149,181,197..198,200..202)
                     /site_type="active"
                     /db_xref="CDD:319823"
     CDS             1..297
                     /gene="LOC144132839"
                     /coded_by="XM_077665526.1:58..951"
                     /db_xref="GeneID:144132839"
ORIGIN      
        1 mdstsiitqv nreeeqltnf ppdrvpsskk prqrgflntl lccfgsnnqg asnpviaeen
       61 gqyspkgkfl lppvryqdmh kicliidlde tlvhssikpi snadfvvpve tggtvhqvyv
      121 lkrpyvdefl qrvgdayecv lftaslakya dpvadqldkw gvfrarlfre scvfhrgnyv
      181 kdlgrlgrdl hrvviidnsp asymfhpgna vpvgswfddm sdtelrdlmp ffdklsrved
      241 vytvlrnsnn aagggggggg spafpapllm ngaalhavva psispdkgph ggavarh
//
LOCUS       XP_077521663             353 aa            linear   INV 25-NOV-2025
DEFINITION  carboxy-terminal domain RNA polymerase II polypeptide A small
            phosphatase 1-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077521663
VERSION     XP_077521663.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665537.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..353
                     /product="carboxy-terminal domain RNA polymerase II
                     polypeptide A small phosphatase 1-like isoform X2"
                     /calculated_mol_wt=38337
     Region          137..296
                     /region_name="HIF-SF_euk"
                     /note="Dullard-like phosphatase domain; TIGR02251"
                     /db_xref="CDD:274055"
     Site            order(143..147,151..152,198..201,205,237,253..254,
                     256..258)
                     /site_type="active"
                     /db_xref="CDD:319823"
     CDS             1..353
                     /gene="LOC144132839"
                     /coded_by="XM_077665537.1:58..1119"
                     /db_xref="GeneID:144132839"
ORIGIN      
        1 mdstsiitqv nreeeqltnf ppdrvpsskk prqrgflntl lccfgsnnqg asnpviaeen
       61 gqyspkasks srqsappcgt stspiaqlqq nqapapvlar eyaaqeytki sssnltvllp
      121 lqgkfllppv ryqdmhkicl iidldetlvh ssikpisnad fvvpvetggt vhqvyvlkrp
      181 yvdeflqrvg dayecvlfta slakyadpva dqldkwgvfr arlfrescvf hrgnyvkdlg
      241 rlgrdlhrvv iidnspasym fhpgnavpvg swfddmsdte lrdlmpffdk lsrvedvytv
      301 lrnsnnaagg ggggggspaf papllmngaa lhavvapsis pdkgphggav arh
//
LOCUS       XP_077521701             131 aa            linear   INV 25-NOV-2025
DEFINITION  F-box only protein 41-like [Amblyomma americanum].
ACCESSION   XP_077521701
VERSION     XP_077521701.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665575.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..131
                     /product="F-box only protein 41-like"
                     /calculated_mol_wt=14455
     CDS             1..131
                     /gene="LOC144132863"
                     /coded_by="XM_077665575.1:204..599"
                     /db_xref="GeneID:144132863"
ORIGIN      
        1 mlgkcwpqlq varvggrslt lasvccvlrs cvnlvsfeid rgreideasa eamveaglqr
       61 iehlyfthtv ishravvilq gacprmkets iilhrrdfgc asalndcvas lkvlqrtkpf
      121 dtmlrlvltd e
//
LOCUS       XP_077521714             601 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132871 [Amblyomma americanum].
ACCESSION   XP_077521714
VERSION     XP_077521714.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665588.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..601
                     /product="uncharacterized protein LOC144132871"
                     /calculated_mol_wt=66772
     Region          <40..132
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     Region          <103..273
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     CDS             1..601
                     /gene="LOC144132871"
                     /coded_by="XM_077665588.1:67..1872"
                     /db_xref="GeneID:144132871"
ORIGIN      
        1 maphplkkfw sllllselvk svelrdveen sfsihrvfhe haerltvlvl mgfnlsvada
       61 thlaalvarn kglkklalvd lyidaasmee ivasieghdg ldeleltalr rmadgksiar
      121 lleahitklr lgvecdwepf ldalvtnidl velaivsrst lafslhrlvr avsisetles
      181 leldldtswa idddriweel aaivgqaral rmlritvsnm tnvalellkn eiarnqclrd
      241 lhfhrcqisc hgamellggl rcnstlqlld laelsgrege yrellqfmvd nrlcdrvsvc
      301 ymgcqawllg kaitnngvrf rnfkfccayg peadivfgvl pclkdtltel sidcnevmcd
      361 tgtqalaklf rdslilenvk ldfpstpdts lvllqglaes rsvssltlsg wmisgtddvv
      421 piafedllrv nssivqltvv qgdlgelkvf lkrlaaglae nhsiselnil hgpdclevcd
      481 aaikqslrvn rtmaclaadl llgaklsraa ayslgcvisc nsmktilrsh tslsddvine
      541 klvgtlsair sellqverly ksearrykpp hysegqrydf evellsdiek ylnlwnvsls
      601 e
//
LOCUS       XP_077521734            1551 aa            linear   INV 25-NOV-2025
DEFINITION  cell adhesion molecule Dscam1-like [Amblyomma americanum].
ACCESSION   XP_077521734
VERSION     XP_077521734.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665608.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1551
                     /product="cell adhesion molecule Dscam1-like"
                     /calculated_mol_wt=168093
     Region          31..130
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          50..54
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          63..67
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          88..92
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          108..113
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          121..125
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          244..320
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          336..421
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          354..358
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409549"
     Region          367..371
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409549"
     Region          389..393
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409549"
     Region          403..408
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409549"
     Region          416..419
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409549"
     Region          427..523
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          445..449
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          458..462
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          489..493
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          503..508
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          516..519
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          526..613
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          543..547
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          556..560
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          578..582
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          592..597
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          606..609
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          617..690
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          634..638
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          648..652
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          673..677
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          711..805
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          728..732
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          741..745
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          770..774
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          784..789
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          798..801
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          809..904
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          826..830
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          839..843
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          870..874
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          <884..1206
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Region          884..889
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          908..1004
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(908,977,992)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(993..994,996..997)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          1210..1291
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     CDS             1..1551
                     /gene="LOC144132890"
                     /coded_by="XM_077665608.1:225..4880"
                     /db_xref="GeneID:144132890"
ORIGIN      
        1 mwspwllvqa alffapatvr gvtlsqppsp plffkeeppg wvpflnstga vvpcsaggsp
       61 pptvtwrkad ggapvtelpg lrqlrpdgsl vfppfgvdqy rpdvhatsyr ceasnsygii
      121 gsrdvrvaav vrtplevsvt dsvasvggva mlrclvppaa reyvsltawv sdddvtvlps
      181 tdvssnrsrf qafasgllvv ngvtrnderr rfrcvvkdsl tgekvesgiw gkfvvtetkg
      241 hsapqllehe gkvrvregtl akipcaatgn pppnyrwykl tgsqihpvvl shkvqevdgt
      301 lvfrraslhd sgkyvciann slgqdradre livtaplrae impkrltvrs gddvtvncty
      361 aggpvhsvhw lkdqrplltd hrvrqlgrlv lhvsgfrrad agmyqcfvan dadsaqghal
      421 llieevapal pdvftaqvhe pgqgvslrct atgsplpqvs wlvdgeplae rrdvqrgdfv
      481 qpdgafvvsy vnisalavtd ggdytcvarn evgaarhtar lsvtgppfir parnltvvag
      541 rpltltcpvv ghpidavhve rdgkplpqes rhtlekpgtl vihearkedq gpyrciavgs
      601 kgdmatrdiy vivvtapvis pfafperlqe gmrssatctl ldgdppvtmg wlkdgrpdlg
      661 pdvqvvpisd fvsnliidkv arhhsgdytc tasnaaaatn htahmfvral prwtirptdl
      721 stvvgssvtf dcqaegephp vvrwkyavgg elrefhsivs sphihvleng sltivsvlse
      781 drgrylceas ngvgpaistt vtltvnnsph vsgmsrvlqa rrgaevtiqc hvtgdepisi
      841 swgrdgspla pfqitkyvlk eensdrgrms tlkiraaers dsatfsctas npfgkdtgss
      901 rvivqevpda pedlrvaevt snvvslvwta pyagnspias ysivyqptgg grhdhdsvrr
      961 isvpggltta svtnlqpvte yqfkviannd lgsstpskpi svttnmeapq eapsqvsaaa
     1021 ldsssiklsw rkphhdhknm qisgyyvgyk pqgtsepfiy ktvevdnkgs keefvigglq
     1081 kntkyvivvq afngqgagpa adeivvktye ydpprspplr viattstsvh vrwepagkds
     1141 vvtgyilhyk haasewkeeq lhsyqtnkil dgllcgttyq lfltafndig rgepspvvev
     1201 ttegkapvap psdsfvsanm ssasvklkaw adggcpirhf vvqykpqstr dwivvsnhiv
     1261 peqqtlqlsd lspgtwhdll avalndagrt eadyqfatlt lqgatvppps aggsssgnrl
     1321 ddaallvpvl caavvlvvia avasfvvvwk rrgdslpdaa sdnyargspg rpddmclsty
     1381 gkttkgdamy esqreslyyp spyatshmay yagsrsaqde gtlrrasrga kehtydvphr
     1441 lkqngtdasn eqyaqlwtss rhpsltggte edfedatspv qyreetyssg klssrfkdga
     1501 crkvpyddlg epeelsetec drdhnlyqyn vknfarqssn idgayqwplq g
//
LOCUS       XP_077521755             269 aa            linear   INV 25-NOV-2025
DEFINITION  putative chitinase [Amblyomma americanum].
ACCESSION   XP_077521755
VERSION     XP_077521755.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665629.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..269
                     /product="putative chitinase"
                     /calculated_mol_wt=29251
     Region          17..>135
                     /region_name="GH18_chitinase-like"
                     /note="The GH18 (glycosyl hydrolase, family 18) type II
                     chitinases hydrolyze chitin, an abundant polymer of
                     beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a
                     major component of the cell wall of fungi and the
                     exoskeleton of arthropods. Chitinases have...; cl10447"
                     /db_xref="CDD:471972"
     Region          <173..213
                     /region_name="Glyco_18"
                     /note="Glyco_18 domain; smart00636"
                     /db_xref="CDD:214753"
     CDS             1..269
                     /gene="LOC144132901"
                     /coded_by="XM_077665629.1:14..823"
                     /db_xref="GeneID:144132901"
ORIGIN      
        1 mganvvvlgm sggrtavvcy rnspsllraa pkdydlgklc ahccthlvyt garvdpnglr
       61 lqfqeddtgl ksvrdmnalk tshpqlrlvl svqhvgsgpl nltegtdaas rralladsvv
      121 hwlgefnfeg veldwdrlpv dlnpvyvdlv ricleissdl svplykeadc kiavnrdkgq
      181 wigydnkati lnnvlfakrm rmggicvvtv dmdyngdcsq rnvllgqlhn alnemdqpik
      241 rlsanlslpe sesssttats rstaamast
//
LOCUS       XP_077521782             174 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132912 [Amblyomma americanum].
ACCESSION   XP_077521782
VERSION     XP_077521782.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665656.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..174
                     /product="uncharacterized protein LOC144132912"
                     /calculated_mol_wt=19531
     Region          <8..>88
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..174
                     /gene="LOC144132912"
                     /coded_by="XM_077665656.1:1..525"
                     /db_xref="GeneID:144132912"
ORIGIN      
        1 mmyskawilq ciimqmkspr lyehmrrqni ltlsskvtlq rylkscrtgf gfnenvlnil
       61 kqktstmdif krhgglivde mklsenlcvs ssghiqgfvd rskftprsem hnvadhetvi
      121 mfvpfagkwt hilacfatrg ntkgdlltkv mleavilaen aglfvdvias dgat
//
LOCUS       XP_077521801             492 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132927 [Amblyomma americanum].
ACCESSION   XP_077521801
VERSION     XP_077521801.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665675.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..492
                     /product="uncharacterized protein LOC144132927"
                     /calculated_mol_wt=55413
     Region          9..103
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          265..>302
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          344..480
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..492
                     /gene="LOC144132927"
                     /coded_by="XM_077665675.1:19..1497"
                     /db_xref="GeneID:144132927"
ORIGIN      
        1 mwngrgktcc vvdckncdrd lkvwdesvce lhgpelhkdc pclrpfamhc fpagernklv
       61 rqrwianlqr kdfnpgkaar vcsidfidgr ptpnnpyrtl hlgtstvrhk grrvlkhrlv
      121 iapmvetsst gastshntsi qhsptqeeee slsetdgtrp tatylrpars ngertaagcq
      181 tcgcrcktvk kmeqavqvdm kgdedhtyca asspqrstea acqaaftvcs tlqphtfkff
      241 tglsknlfde lvralastmk tpfelpfedq vlltlmrlrl gllvhdlsfr faistaavsr
      301 ifctissgla kfarqylvfw lardtikasi pdsfnryels tciidcfeif ierpgnlkrr
      361 nrtysryksh ntakvlhaia pcgfvmfvsk pyggrasdrf itqhsgflkf lapgdtilad
      421 rgftisdllp agvklmlpsf skgrsqisrp elvesrrlar lrihversir rikcfrllke
      481 lpsrffakkt tg
//
LOCUS       XP_077521812             185 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132937 [Amblyomma americanum].
ACCESSION   XP_077521812
VERSION     XP_077521812.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665686.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..185
                     /product="uncharacterized protein LOC144132937"
                     /calculated_mol_wt=20575
     CDS             1..185
                     /gene="LOC144132937"
                     /coded_by="XM_077665686.1:420..977"
                     /db_xref="GeneID:144132937"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedrrl tatmkrlsgv hvlnhehrfl
       61 dasgepmlsl faadrgidqm skiivaalvk iygpgiasas rarpgevyvv hrcrrcgakg
      121 hktdhcgpta hraatplqva vevllqtalf ratslfpgrs rasrfvpvfp lcrnrrqtsk
      181 srgvs
//
LOCUS       XP_077521820             185 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132937 [Amblyomma americanum].
ACCESSION   XP_077521820
VERSION     XP_077521820.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665694.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..185
                     /product="uncharacterized protein LOC144132937"
                     /calculated_mol_wt=20575
     CDS             1..185
                     /gene="LOC144132937"
                     /coded_by="XM_077665694.1:355..912"
                     /db_xref="GeneID:144132937"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedrrl tatmkrlsgv hvlnhehrfl
       61 dasgepmlsl faadrgidqm skiivaalvk iygpgiasas rarpgevyvv hrcrrcgakg
      121 hktdhcgpta hraatplqva vevllqtalf ratslfpgrs rasrfvpvfp lcrnrrqtsk
      181 srgvs
//
LOCUS       XP_077521832             140 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132949 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077521832
VERSION     XP_077521832.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665706.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..140
                     /product="uncharacterized protein LOC144132949 isoform X1"
                     /calculated_mol_wt=14845
     CDS             1..140
                     /gene="LOC144132949"
                     /coded_by="XM_077665706.1:342..764"
                     /db_xref="GeneID:144132949"
ORIGIN      
        1 mqyftrglpl psgqqkqlyy avpvasaqev taegivatql esscyqgnat ystmsekmht
       61 mmlgvsilti lpilstneak dasvqiipta tmegknrvaa fvrgretgma vgkldegggp
      121 ftpvtgalqe cprclartnk
//
LOCUS       XP_077521839             140 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132949 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077521839
VERSION     XP_077521839.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665713.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..140
                     /product="uncharacterized protein LOC144132949 isoform X1"
                     /calculated_mol_wt=14845
     CDS             1..140
                     /gene="LOC144132949"
                     /coded_by="XM_077665713.1:236..658"
                     /db_xref="GeneID:144132949"
ORIGIN      
        1 mqyftrglpl psgqqkqlyy avpvasaqev taegivatql esscyqgnat ystmsekmht
       61 mmlgvsilti lpilstneak dasvqiipta tmegknrvaa fvrgretgma vgkldegggp
      121 ftpvtgalqe cprclartnk
//
LOCUS       XP_077521849             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132949 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077521849
VERSION     XP_077521849.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665723.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144132949 isoform X2"
                     /calculated_mol_wt=13971
     CDS             1..127
                     /gene="LOC144132949"
                     /coded_by="XM_077665723.1:259..642"
                     /db_xref="GeneID:144132949"
ORIGIN      
        1 mrrvfvssii nrtmaatcsw fsghvkgkkt gvsefkveka mydtmkgipk davfhkrtai
       61 tiwsteavvl csssgkcsgg dsgrhcgyta gvkllsgkcn lfdyvrkdah ydarclhldy
      121 tanfvyq
//
LOCUS       XP_077521857             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132949 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077521857
VERSION     XP_077521857.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665731.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144132949 isoform X2"
                     /calculated_mol_wt=13971
     CDS             1..127
                     /gene="LOC144132949"
                     /coded_by="XM_077665731.1:259..642"
                     /db_xref="GeneID:144132949"
ORIGIN      
        1 mrrvfvssii nrtmaatcsw fsghvkgkkt gvsefkveka mydtmkgipk davfhkrtai
       61 tiwsteavvl csssgkcsgg dsgrhcgyta gvkllsgkcn lfdyvrkdah ydarclhldy
      121 tanfvyq
//
LOCUS       XP_077521865             125 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132949 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077521865
VERSION     XP_077521865.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665739.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..125
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..125
                     /product="uncharacterized protein LOC144132949 isoform X3"
                     /calculated_mol_wt=13699
     CDS             1..125
                     /gene="LOC144132949"
                     /coded_by="XM_077665739.1:342..719"
                     /db_xref="GeneID:144132949"
ORIGIN      
        1 mqyftrglpl psgqqkqlyy avpvasaqev taegivatql esscyqgnat ystmsekmht
       61 mmlgvsilti lpilstneak dasvqiaesq qppwkartew lrscggerlg wrseswmkgv
      121 dpspq
//
LOCUS       XP_077521874              99 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132949 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077521874
VERSION     XP_077521874.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665748.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..99
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..99
                     /product="uncharacterized protein LOC144132949 isoform X4"
                     /calculated_mol_wt=10821
     CDS             1..99
                     /gene="LOC144132949"
                     /coded_by="XM_077665748.1:160..459"
                     /db_xref="GeneID:144132949"
ORIGIN      
        1 mtgvsefkve kamydtmkgi pkdavfhkrt aitiwsteav vlcsssgkcs ggdsgrhcgy
       61 tagvkllsgk cnlfdyvrkd ahydarclhl dytanfvyq
//
LOCUS       XP_077521884             391 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132983 [Amblyomma americanum].
ACCESSION   XP_077521884
VERSION     XP_077521884.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665758.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..391
                     /product="uncharacterized protein LOC144132983"
                     /calculated_mol_wt=42780
     Region          <170..>367
                     /region_name="COG4372"
                     /note="Uncharacterized protein, contains DUF3084 domain
                     [Function unknown]"
                     /db_xref="CDD:443500"
     CDS             1..391
                     /gene="LOC144132983"
                     /coded_by="XM_077665758.1:2..1177"
                     /db_xref="GeneID:144132983"
ORIGIN      
        1 mqttvlafas lllpslagls tgassvcalp ytgddpgsch lppnecssll dvppeelhtp
       61 eitvpwvats tsatmptcik elhpltplrg lgdcttaqpp npltfamqvs tsyalfakks
      121 snyilvqfps phccvaiave cahvihsllm lsgdvetspg pegnaavlae lqklnagqtl
      181 liteiqglkt qlnttdqtia sldkrmadle ahyqtllhlr ndieimqstt inqakkieel
      241 etrlddaenq srrnniifyg ipdpasaetw aeseklvidv crnnlditle pndierahrl
      301 gnhsadrtrp vivkflshkt kdallsngrk lkdtnysige dfsrtvrhar kqllvfakan
      361 snkyslrfkt lhigskryvf dassqtvkei a
//
LOCUS       XP_077521897             143 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132991 [Amblyomma americanum].
ACCESSION   XP_077521897
VERSION     XP_077521897.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665771.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..143
                     /product="uncharacterized protein LOC144132991"
                     /calculated_mol_wt=16154
     CDS             1..143
                     /gene="LOC144132991"
                     /coded_by="XM_077665771.1:6..437"
                     /db_xref="GeneID:144132991"
ORIGIN      
        1 mtlatrvsts qrewrgapfp vegdeseegi estmtpldee hrpveecqvs kgamrgiqnt
       61 dplwfgeltl dpqsvkqprs pqevggltgq rkaarvepdr eewrirvpep dgalvvqlwr
      121 htllratvlr prrlptpfrp srp
//
LOCUS       XP_077521910             603 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144132999 [Amblyomma americanum].
ACCESSION   XP_077521910
VERSION     XP_077521910.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665784.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..603
                     /product="uncharacterized protein LOC144132999"
                     /calculated_mol_wt=67715
     Region          <254..314
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     Region          <302..585
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..603
                     /gene="LOC144132999"
                     /coded_by="XM_077665784.1:1..1812"
                     /db_xref="GeneID:144132999"
ORIGIN      
        1 mevtvegtei tpeelrtsdw itkvgkhvke lhdltrgerr ggkdgdarle rgaesgegkk
       61 iaaeanaayk klgqknaers iasylprlpa ndykiiirpk nglalakvpt trlsaavrma
      121 aqipwvkgqd kdvlihndkq gtlifstpdm dtvwkvtkik sihidddeye vtaclaphed
      181 cgrgvvhgid pkltieelte afgtprnppi lgvrkmgkss saivifkhet vprwvycydv
      241 plkcvlykkr yevcyrcgel ghrsdvcvsa qvkclgcgii appedhvcep kcklcgkghl
      301 tadrkckeaf rtpytikkrq weaklgmevm eeearkaket evgssrfekh qgrsrsqsgn
      361 qsesrgrses fprlpnlrea ekqkkaqpgt agpsggavvn nparpkspsr kvgwvseasq
      421 dkrdeeifqi keenrllkaq laamskqiee lrialkpnaa pakqsqmpqn pvvraseeqg
      481 aaqppakkra kevekpiidm dtekvidmki eqleakfeak srqenewrka feermlgmfq
      541 tlseqlhqrd nsvaeqihqr dnnlteqlke efakrdraye hltqqvlgnq mnqliashgs
      601 qiq
//
LOCUS       XP_077521922             160 aa            linear   INV 25-NOV-2025
DEFINITION  importin-7-like [Amblyomma americanum].
ACCESSION   XP_077521922
VERSION     XP_077521922.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665796.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..160
                     /product="importin-7-like"
                     /calculated_mol_wt=17624
     CDS             1..160
                     /gene="LOC144133007"
                     /coded_by="XM_077665796.1:105..587"
                     /db_xref="GeneID:144133007"
ORIGIN      
        1 mmqtttttrm tlttsarsvr relwgapfpa psasiegdra egndseqgia snttprnede
       61 cpvdkyqvfk evmrgiqkad plwfvaltsg pqfhvqqrsp qevnvltghk kaaaesirie
      121 ksggyvfqnq tasssfssgg trfvkpqdfe regfhelgwp
//
LOCUS       XP_077521931             355 aa            linear   INV 25-NOV-2025
DEFINITION  G-protein coupled receptor 83-like [Amblyomma americanum].
ACCESSION   XP_077521931
VERSION     XP_077521931.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665805.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..355
                     /product="G-protein coupled receptor 83-like"
                     /calculated_mol_wt=39847
     Region          13..298
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          15..39
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320511"
     Region          48..70
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320511"
     Region          86..108
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320511"
     Region          128..144
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320511"
     Region          175..198
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320511"
     Region          229..251
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320511"
     Region          266..291
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320511"
     CDS             1..355
                     /gene="LOC144133016"
                     /coded_by="XM_077665805.1:72..1139"
                     /db_xref="GeneID:144133016"
ORIGIN      
        1 mearpllrkp rdvlvillyg lvvlvslfgn tlvchavlrs rkmrtktnvl ianlavsdll
       61 mttlsiplsa srflldnwpf gealcylapf lqvtfvyvst ltmawiacdr ygvivhplrl
      121 rrlcpahgai aciwtlagal slphavfnrv vslftyrtlv rsqvqypaps vtfrqwltla
      181 tfltqyalpl altafmcyrv sralwahkal galtrkqqal htrskrkslk mlvlvvacfa
      241 vcwlplnvyh lvahfraddg pdrhnsnlfi sfhwlamssv cynpfiycwl nnkfrqgala
      301 clgflprcpq rkpqpwspkk saaltrgglr gshlskrcst lkstslgsad ritcv
//
LOCUS       XP_077521944             187 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133024 [Amblyomma americanum].
ACCESSION   XP_077521944
VERSION     XP_077521944.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665818.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="uncharacterized protein LOC144133024"
                     /calculated_mol_wt=21494
     Region          <16..>92
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          19..39
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(19,22,35,39)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(24,26,28,30..31,34..35,38,52,54,58..59,62..63,66,80,
                     82,84,86..87,90..91)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          47..67
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          59..82
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          75..91
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          104..121
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..187
                     /gene="LOC144133024"
                     /coded_by="XM_077665818.1:22..585"
                     /db_xref="GeneID:144133024"
ORIGIN      
        1 memhtrqkhw rntkgihqct hctftstrka diirherthv dkrnsvcnlc qkrfsrpdnl
       61 kvhmmihtrd kpyecgqcgk rfreraharr heevihsrry plhcprcgag avdvtrlrrh
      121 ickqlgtteg kegfkrgrgr prlmadgtap tvrprntteg aplqggvkrg rgqprkalaq
      181 dasmprp
//
LOCUS       XP_077521964             151 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133031 [Amblyomma americanum].
ACCESSION   XP_077521964
VERSION     XP_077521964.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665838.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..151
                     /product="uncharacterized protein LOC144133031"
                     /calculated_mol_wt=16408
     Region          6..>80
                     /region_name="SWIM"
                     /note="Uncharacterized protein, contains SWIM-type Zn
                     finger domain [Function unknown]; COG4715"
                     /db_xref="CDD:443750"
     CDS             1..151
                     /gene="LOC144133031"
                     /coded_by="XM_077665838.1:32..487"
                     /db_xref="GeneID:144133031"
ORIGIN      
        1 mkggyyivha vaekttgdit eahchcpagl sgtcqhvvgl llavvgaaae peptctdlpc
       61 awivpssakk vevakplsdi tfkttdpngr qgfkrkrsyd pcpngmpsda gcfrrklqna
      121 fpralwlryn keateqvffp mvlqifvqii t
//
LOCUS       XP_077521979             174 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133035 [Amblyomma americanum].
ACCESSION   XP_077521979
VERSION     XP_077521979.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665853.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..174
                     /product="uncharacterized protein LOC144133035"
                     /calculated_mol_wt=18734
     CDS             1..174
                     /gene="LOC144133035"
                     /coded_by="XM_077665853.1:21..545"
                     /db_xref="GeneID:144133035"
ORIGIN      
        1 mlytksgqga ddvyigkwkf fkammfidng apaesrtyte tygmateeey gqspenslvv
       61 esqdpavdas vsntaaaeap vpqaseaqsa valananqaa pkrkriadhq lallqqrtdt
      121 lskmaktldn sspddcsafa svlahyfrkl plakrakcqa avltlmttyl edes
//
LOCUS       XP_077522003             160 aa            linear   INV 25-NOV-2025
DEFINITION  importin-7-like [Amblyomma americanum].
ACCESSION   XP_077522003
VERSION     XP_077522003.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665877.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..160
                     /product="importin-7-like"
                     /calculated_mol_wt=17693
     CDS             1..160
                     /gene="LOC144133047"
                     /coded_by="XM_077665877.1:17..499"
                     /db_xref="GeneID:144133047"
ORIGIN      
        1 mrqtttttrm tlttsarsvr rewwgapfpa psasiegdra egndseqgia ssttprnede
       61 cpvdkyqvfk evmrgiqkad plwfvaltsg pqflvqqrsp qkvnvltghr kaaaesirie
      121 ksggyvfqnq tapssfssgg trfvkpqnfe regfhdlgwp
//
LOCUS       XP_077522013             409 aa            linear   INV 25-NOV-2025
DEFINITION  G-protein coupled receptor 83-like [Amblyomma americanum].
ACCESSION   XP_077522013
VERSION     XP_077522013.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665887.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..409
                     /product="G-protein coupled receptor 83-like"
                     /calculated_mol_wt=45310
     Region          67..352
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          69..93
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320511"
     Region          102..124
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320511"
     Region          140..162
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320511"
     Region          182..198
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320511"
     Region          229..252
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320511"
     Region          283..305
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320511"
     Region          320..345
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320511"
     CDS             1..409
                     /gene="LOC144133057"
                     /coded_by="XM_077665887.1:74..1303"
                     /db_xref="GeneID:144133057"
ORIGIN      
        1 mcplsaavnp rvtdqaepsg mgpasasfss pgdlcgaeqs anlsalcsll rnlsmearpl
       61 lrkprdvlvi llyglvvlvs lfgntlvcha vlrsrkmrtk tnvlianlav sdllmttlti
      121 plsasrflld nwpfgealcy lapflqvtfv yvstltmawi acdrygvivh plrlrrlcpa
      181 hgaiaciwtl agalslphav fnrvvslfty rtlvrcqvqy papsvtfrqw ltlatfltqy
      241 alplaltafm yyrvsralwa rkalgaltre qqalhtrskr kslkmlvlvv acfavcwlpl
      301 nvyhlvahfr addgpdrhns nlfisfhwla mssvcynpfi ycwlnnkfrq galaclgflp
      361 rcsqrkpqpw spkksaaltr gglrgshlsk rcstlkstsl gsadritcv
//
LOCUS       XP_077522025             197 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133065 [Amblyomma americanum].
ACCESSION   XP_077522025
VERSION     XP_077522025.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665899.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..197
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..197
                     /product="uncharacterized protein LOC144133065"
                     /calculated_mol_wt=21679
     Region          <4..38
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..197
                     /gene="LOC144133065"
                     /coded_by="XM_077665899.1:1..594"
                     /db_xref="GeneID:144133065"
ORIGIN      
        1 maaariqrwa hhlgayryrl qyapgrqmln adalsrvphr ypqadvdslp gpkennddll
       61 plllsevyar nygagsiwtl chvettsgar mvtvetsdgv vqrhvcqvrp rpeaprdnpe
      121 vttntthcsa sdqatqlqhp etvspdegia arrtpngtgs pdlpapdipv aplntgvaqq
      181 tlrrstrehk pvqrfhf
//
LOCUS       XP_077522085             198 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133101 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077522085
VERSION     XP_077522085.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665959.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..198
                     /product="uncharacterized protein LOC144133101 isoform X1"
                     /calculated_mol_wt=22590
     CDS             1..198
                     /gene="LOC144133101"
                     /coded_by="XM_077665959.1:46..642"
                     /db_xref="GeneID:144133101"
ORIGIN      
        1 mpamcarrrv lllvirtalq livrrrqerr qrrrhrwwvr pvfvarrqeg ryhptmrrmr
       61 egdheffrkf frmtpvlfde vlgfvlpdlt rqhvvreple pgerlaials kptgawrdgt
      121 dwldagdswr dgsdswrdgs gssvttscts dacdaqtrrh ssnsmmvsdg sgallcwyag
      181 vllapgttfg pgslnccl
//
LOCUS       XP_077522090             172 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133101 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077522090
VERSION     XP_077522090.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665964.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..172
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..172
                     /product="uncharacterized protein LOC144133101 isoform X2"
                     /calculated_mol_wt=19463
     CDS             1..172
                     /gene="LOC144133101"
                     /coded_by="XM_077665964.1:46..564"
                     /db_xref="GeneID:144133101"
ORIGIN      
        1 mpamcarrrv lllvirtalq livrrrqerr qrrrhrwwvr pvfvarrqeg ryhptmrrmr
       61 edltrqhvvr eplepgerla ialskptgaw rdgtdwldag dswrdgsdsw rdgsgssvtt
      121 sctsdacdaq trrhssnsmm vsdgsgallc wyagvllapg ttfgpgslnc cl
//
LOCUS       XP_077522101             438 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133114 [Amblyomma americanum].
ACCESSION   XP_077522101
VERSION     XP_077522101.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665975.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..438
                     /product="uncharacterized protein LOC144133114"
                     /calculated_mol_wt=46219
     Region          <230..>318
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     Region          <324..408
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..438
                     /gene="LOC144133114"
                     /coded_by="XM_077665975.1:198..1514"
                     /db_xref="GeneID:144133114"
ORIGIN      
        1 malpeqrgyd pttlswssvp asqpedgpst isaqqaalfq vvesrrrrrq nargaatpkt
       61 pldksatgda rapsskgpqk sfspvskwtp kptvrmnpdd yvivlrprst valktafqqg
      121 elgaaiseli grqhmnaiti spnweqniiv cgtqnpevaq klltdiqitt skgslplhgh
      181 lkltgdvcrg visvhnlets eslkhsvqwr ggdlvhvrkl gksnvavltf egrsvpryvh
      241 ynaeltpvke ykktvpacyr cgtlghrvdn cptpvkercg lcgqnvglgt egmaphvcnp
      301 mclicggphl tssrectakf iklpqpgprt pskgansgat rggrqqnnqk tprrgvmppp
      361 gktatvtppp gaaefpalga spggaacnqp krglgggpar lsrpeepkgl gstdaggvac
      421 qwgpgigapp stvrgkgq
//
LOCUS       XP_077522112             134 aa            linear   INV 25-NOV-2025
DEFINITION  importin-7-like [Amblyomma americanum].
ACCESSION   XP_077522112
VERSION     XP_077522112.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665986.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..134
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..134
                     /product="importin-7-like"
                     /calculated_mol_wt=14574
     CDS             1..134
                     /gene="LOC144133124"
                     /coded_by="XM_077665986.1:30..434"
                     /db_xref="GeneID:144133124"
ORIGIN      
        1 mrlptkdsts qggwlgilsp assastrddp vesvkpeqgm eqeqcpvdey pdskevmggi
       61 qntdpmwfga ltsgtqsveq krspqevnvl tdqrkaates rrieksggyv fqnqagpppf
      121 rsggtpfrqq lnfl
//
LOCUS       XP_077522124             141 aa            linear   INV 25-NOV-2025
DEFINITION  importin-7-like [Amblyomma americanum].
ACCESSION   XP_077522124
VERSION     XP_077522124.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077665998.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="importin-7-like"
                     /calculated_mol_wt=15372
     CDS             1..141
                     /gene="LOC144133133"
                     /coded_by="XM_077665998.1:6..431"
                     /db_xref="GeneID:144133133"
ORIGIN      
        1 mrlptkdsts qggwlgvlsp assastrddp vesvkpeqgm estttpldee qcpvdeypds
       61 kevmrgiqnt dpmwfgalts gpqsveqkrs pqevnvltdq rkaatesrri eksggyvfqn
      121 qagpppfrsg gtpfrqqlnf l
//
LOCUS       XP_077522143              96 aa            linear   INV 25-NOV-2025
DEFINITION  importin-7-like [Amblyomma americanum].
ACCESSION   XP_077522143
VERSION     XP_077522143.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666017.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..96
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..96
                     /product="importin-7-like"
                     /calculated_mol_wt=10540
     CDS             1..96
                     /gene="LOC144133149"
                     /coded_by="XM_077666017.1:79..369"
                     /db_xref="GeneID:144133149"
ORIGIN      
        1 mestttllhe eqcpvheypv skevmraipn tdpmcfgalt sgpqfveqkr spqeicvltd
       61 qtkaaaesnr iekrggyvfq nqtlpppfss ggtpfr
//
LOCUS       XP_077522156              96 aa            linear   INV 25-NOV-2025
DEFINITION  importin-7-like [Amblyomma americanum].
ACCESSION   XP_077522156
VERSION     XP_077522156.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666030.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..96
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..96
                     /product="importin-7-like"
                     /calculated_mol_wt=10476
     CDS             1..96
                     /gene="LOC144133153"
                     /coded_by="XM_077666030.1:38..328"
                     /db_xref="GeneID:144133153"
ORIGIN      
        1 mesittllhe eqcpvdeypv akevmraiqn tdpmcfgalt sgpqfveqkr spqeicvltd
       61 qtkaaaesnr ieksggyvfq nqtlpppfss ggtpfr
//
LOCUS       XP_077522167             122 aa            linear   INV 25-NOV-2025
DEFINITION  importin-7-like [Amblyomma americanum].
ACCESSION   XP_077522167
VERSION     XP_077522167.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666041.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..122
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..122
                     /product="importin-7-like"
                     /calculated_mol_wt=13538
     CDS             1..122
                     /gene="LOC144133157"
                     /coded_by="XM_077666041.1:61..429"
                     /db_xref="GeneID:144133157"
ORIGIN      
        1 mestttllhe eqcpvheypv sktvmraiqn tdpmwfgalt lgpksveqkr spkevcvltd
       61 qtksaaesnr ieksggyvfq nqtvpppfps ggttfrqpln fyrsttcdre tptstflcgf
      121 vf
//
LOCUS       XP_077522196              96 aa            linear   INV 25-NOV-2025
DEFINITION  importin-7-like [Amblyomma americanum].
ACCESSION   XP_077522196
VERSION     XP_077522196.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666070.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..96
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..96
                     /product="importin-7-like"
                     /calculated_mol_wt=10476
     CDS             1..96
                     /gene="LOC144133181"
                     /coded_by="XM_077666070.1:38..328"
                     /db_xref="GeneID:144133181"
ORIGIN      
        1 mesittllhe eqcpvdeypv akevmraiqn tdpmcfgalt sgpqfveqkr spqeicvltd
       61 qtkaaaesnr ieksggyvfq nqtlpppfss ggtpfr
//
LOCUS       XP_077522207             122 aa            linear   INV 25-NOV-2025
DEFINITION  importin-7-like [Amblyomma americanum].
ACCESSION   XP_077522207
VERSION     XP_077522207.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666081.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..122
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..122
                     /product="importin-7-like"
                     /calculated_mol_wt=13538
     CDS             1..122
                     /gene="LOC144133187"
                     /coded_by="XM_077666081.1:61..429"
                     /db_xref="GeneID:144133187"
ORIGIN      
        1 mestttllhe eqcpvheypv sktvmraiqn tdpmwfgalt lgpksveqkr spkevcvltd
       61 qtksaaesnr ieksggyvfq nqtvpppfps ggttfrqpln fyrsttcdre tptstflcgf
      121 vf
//
LOCUS       XP_077522235             162 aa            linear   INV 25-NOV-2025
DEFINITION  putative JmjC domain-containing histone demethylation protein 2C
            isoform X1 [Amblyomma americanum].
ACCESSION   XP_077522235
VERSION     XP_077522235.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666109.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..162
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..162
                     /product="putative JmjC domain-containing histone
                     demethylation protein 2C isoform X1"
                     /calculated_mol_wt=17844
     CDS             1..162
                     /gene="LOC144133212"
                     /coded_by="XM_077666109.1:147..635"
                     /db_xref="GeneID:144133212"
ORIGIN      
        1 maykhreeiv gkrflcvnsa gkaklskppd ldwragiira ashkdpkhpe lsvlvefdna
       61 dwkrrewirv yedpfaaflv eetltwhvrn pdetpspalv sepppspqpl pargamtlag
      121 cpphhgrggp vqplsacawl sprvvpacav grsncrrvrh gv
//
LOCUS       XP_077522244             114 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133212 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077522244
VERSION     XP_077522244.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666118.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..114
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..114
                     /product="uncharacterized protein LOC144133212 isoform X2"
                     /calculated_mol_wt=12446
     CDS             1..114
                     /gene="LOC144133212"
                     /coded_by="XM_077666118.1:333..677"
                     /db_xref="GeneID:144133212"
ORIGIN      
        1 mippvlvefd nadwkrrewi rvyedpfaaf lveetltwhv rnpdetpspa lvsepppspq
       61 plpargamtl agcpphhgrg gpvqplsaca wlsprvvpac avgrsncrrv rhgv
//
LOCUS       XP_077522264             716 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133228 [Amblyomma americanum].
ACCESSION   XP_077522264
VERSION     XP_077522264.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666138.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..716
                     /product="uncharacterized protein LOC144133228"
                     /calculated_mol_wt=77734
     Region          <144..375
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     Region          <258..548
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     CDS             1..716
                     /gene="LOC144133228"
                     /coded_by="XM_077666138.1:89..2239"
                     /db_xref="GeneID:144133228"
ORIGIN      
        1 magagnhypw rcrlerllhg lpserscsag eleecwligd raswnkalra trlmltefep
       61 gklclrsyvd qddqrashhg sgnendgafl lawlprqhsc icevrvhdrt hierlsfiss
      121 vivgpannlq svvlhasyrl lpesrflqaf gppqclreld ltnvhisdal apkvadvlrg
      181 ngqtlqsvkl ignnlsresa stlllalvac erledlalqd sressacdvm aeafkssrll
      241 qklslvglce tgmsckhlsg ergcldclad lfgalegils fqnlalkkts isdtlgesla
      301 kfltghhspq tlsllncsfd srgaaciaea lsancplehl slqdscldaa tvsklcaalp
      361 antalkslvl pqisstsril qeknsileql rlecpgrlqf slfvdrsssv saplaqalgc
      421 atnvkiwhwg lvgkvacfvy aslalnhslr slhvdygcfv pgyhgarqga ltsalcaalk
      481 acgslqsfhl dahrafiesd ettqlmteif dalqcnprlr kltldisqlt vkaarllsvl
      541 vgqfkrsfve lriessgnms saalgvlqem itknvflsrv svkcpawkda vgacaaieda
      601 keqnqgllnk aakfvmsidg rptasakhpc asafeelcgt aslqehlvsl sgksepqvst
      661 dvkkarcyld dnymiyagvv rakvlceagd gstqfddltv hcwrcivqyl klsdvv
//
LOCUS       XP_077522275             232 aa            linear   INV 25-NOV-2025
DEFINITION  endoplasmic reticulum-Golgi intermediate compartment protein 1-like
            isoform X1 [Amblyomma americanum].
ACCESSION   XP_077522275
VERSION     XP_077522275.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666149.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..232
                     /product="endoplasmic reticulum-Golgi intermediate
                     compartment protein 1-like isoform X1"
                     /calculated_mol_wt=26018
     Region          <29..68
                     /region_name="ERGIC_N"
                     /note="Endoplasmic Reticulum-Golgi Intermediate
                     Compartment (ERGIC); pfam13850"
                     /db_xref="CDD:464000"
     CDS             1..232
                     /gene="LOC144133236"
                     /coded_by="XM_077666149.1:529..1227"
                     /db_xref="GeneID:144133236"
ORIGIN      
        1 mscvakdgrd wctnearrqr lrrrseslvs elsvdnpsst ekipvsinit llkldcsvvg
       61 ldiqddmgrd eagfvensek tragfgcrfe gkffthkvpg kfhvsthaaa kqpekiyrth
      121 iihdptfvvk mtdevkgsfn sldeidksga nelrdhpahg shhachlvpl rphshhsqvh
      181 tslrdsvqlp dleslhggla aehrrtgmsa rrrrtvgaem vtrrrgirrr lv
//
LOCUS       XP_077522284             232 aa            linear   INV 25-NOV-2025
DEFINITION  endoplasmic reticulum-Golgi intermediate compartment protein 1-like
            isoform X1 [Amblyomma americanum].
ACCESSION   XP_077522284
VERSION     XP_077522284.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666158.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..232
                     /product="endoplasmic reticulum-Golgi intermediate
                     compartment protein 1-like isoform X1"
                     /calculated_mol_wt=26018
     Region          <29..68
                     /region_name="ERGIC_N"
                     /note="Endoplasmic Reticulum-Golgi Intermediate
                     Compartment (ERGIC); pfam13850"
                     /db_xref="CDD:464000"
     CDS             1..232
                     /gene="LOC144133236"
                     /coded_by="XM_077666158.1:319..1017"
                     /db_xref="GeneID:144133236"
ORIGIN      
        1 mscvakdgrd wctnearrqr lrrrseslvs elsvdnpsst ekipvsinit llkldcsvvg
       61 ldiqddmgrd eagfvensek tragfgcrfe gkffthkvpg kfhvsthaaa kqpekiyrth
      121 iihdptfvvk mtdevkgsfn sldeidksga nelrdhpahg shhachlvpl rphshhsqvh
      181 tslrdsvqlp dleslhggla aehrrtgmsa rrrrtvgaem vtrrrgirrr lv
//
LOCUS       XP_077522288             209 aa            linear   INV 25-NOV-2025
DEFINITION  endoplasmic reticulum-Golgi intermediate compartment protein 1-like
            isoform X2 [Amblyomma americanum].
ACCESSION   XP_077522288
VERSION     XP_077522288.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666162.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..209
                     /product="endoplasmic reticulum-Golgi intermediate
                     compartment protein 1-like isoform X2"
                     /calculated_mol_wt=23213
     Region          <1..45
                     /region_name="ERGIC_N"
                     /note="Endoplasmic Reticulum-Golgi Intermediate
                     Compartment (ERGIC); pfam13850"
                     /db_xref="CDD:464000"
     CDS             1..209
                     /gene="LOC144133236"
                     /coded_by="XM_077666162.1:311..940"
                     /db_xref="GeneID:144133236"
ORIGIN      
        1 mspelvsels vdnpssteki pvsinitllk ldcsvvgldi qddmgrdeag fvensektra
       61 gfgcrfegkf fthkvpgkfh vsthaaakqp ekiyrthiih dptfvvkmtd evkgsfnsld
      121 eidksganel rdhpahgshh achlvplrph shhsqvhtsl rdsvqlpdle slhgglaaeh
      181 rrtgmsarrr rtvgaemvtr rrgirrrlv
//
LOCUS       XP_077522313             902 aa            linear   INV 25-NOV-2025
DEFINITION  ionotropic receptor 25a [Amblyomma americanum].
ACCESSION   XP_077522313
VERSION     XP_077522313.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666187.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..902
                     /product="ionotropic receptor 25a"
                     /calculated_mol_wt=102552
     Region          31..402
                     /region_name="Periplasmic_Binding_Protein_type1"
                     /note="Type 1 periplasmic binding fold superfamily;
                     cl10011"
                     /db_xref="CDD:471960"
     Region          425..827
                     /region_name="PBP2_iGluR_putative"
                     /note="The ligand-binding domain of putative ionotropic
                     glutamate receptors, a member of the type 2 periplasmic
                     binding fold protein superfamily; cd13717"
                     /db_xref="CDD:270435"
     Site            order(449,461,465,512..513,516,521..524,788,806..807,
                     810..815,817,820)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:270435"
     Site            order(480,508..510,515,676,679..681,765,791)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270435"
     CDS             1..902
                     /gene="Ir25a"
                     /coded_by="XM_077666187.1:1..2709"
                     /db_xref="GeneID:144133259"
ORIGIN      
        1 milaaarprt miaaflaatl tvvsaqdavh fvvvveknnd vakifitetl kkatdasvty
       61 alveldpdea tvadvcsaif adprpdvlid avrtpgstvd klstavrdta rrmglptlsl
      121 tygvhndytl sgwdrltale reylvhitpp gdiytqvird lckemdlnta giiydssvii
      181 dhkyarllen iptrhimrev dgteddfrkk aelihstdvs nffilgraet lnealklahr
      241 nkwnnwqfaw flisrenysa kcadcnnvta ivatpmaanp dklaeakkdk dfdkhreqmd
      301 asfytdvtrm iikaaaeera srklpvdley pscsnmnlts amlmtrrnit lrttlmqmeh
      361 egpfgpvvir dkengyqeir mavdrvnirr qkrvftarva nwtykdpegv lskpaeedlk
      421 kyaavtvfrv ttivqppfiv kkntsggedq fegycidlid eikkilkfey tiyevqdkmf
      481 gsintttkqw ngmirelvdk nadialgpis vmaeretvvd ftvpyydlvg isilmkkpev
      541 ksslfkfltv legnvwgcil aayfftsflm ylfdrlspys yrnnkekykd deekrdfnlk
      601 eclwfcmtsl tpqgggeapr nlsgrlvaat wwlfgfiiia sytanlaafl tvsrldspie
      661 slddlakqyk ikyapqkdts smtyfermay ieskfydiwk nmslddtlsd eeraklavwd
      721 ypvsdkytki wwtmqeanmp tefeegvrrv keskgsqegf afiadstrvk yatmtncdlv
      781 svgsefsrkp lalavqqnsp lkdqlssail kllnqrrleg lketwwnknp arvtcddser
      841 qsdgisirni ggvfivifig vvmacitlai eycyyknrkp nskvvavkey sgkdsikdsi
      901 kh
//
LOCUS       XP_077522327             812 aa            linear   INV 25-NOV-2025
DEFINITION  ionotropic receptor 93a-like [Amblyomma americanum].
ACCESSION   XP_077522327
VERSION     XP_077522327.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666201.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..812
                     /product="ionotropic receptor 93a-like"
                     /calculated_mol_wt=91743
     Region          320..>548
                     /region_name="Periplasmic_Binding_Protein_Type_2"
                     /note="Type 2 periplasmic binding fold superfamily;
                     cl21456"
                     /db_xref="CDD:473866"
     Region          440..711
                     /region_name="Lig_chan"
                     /note="Ligand-gated ion channel; pfam00060"
                     /db_xref="CDD:459656"
     CDS             1..812
                     /gene="LOC144133264"
                     /coded_by="XM_077666201.1:150..2588"
                     /db_xref="GeneID:144133264"
ORIGIN      
        1 mgrslldaaf tvvnctatqh afevvkvrmp ktflvsimdr fcprlednfa lgfpvtsgpl
       61 dvvpllsdlr sardlrywks iimlhdpdyn pvyvedmvka vrglspkvkp aavttfrvcl
      121 gnpcehgtas insiidkfse eihvrhyiiv gnltlvretl tkmrdanmmt fhrdfvciit
      181 erineefetf tktfpdganv lvayaeveet dcpvenycql piavetvakt igdklakgty
      241 rtsefmftkf ifantsksll lessqcgqcs rftlktvakl rgeqvlldvg hwalvtgirt
      301 erkdffpgii gdlggiklvi avindppmsv vemspdgrtv rnvtgtmaem vealarglnf
      361 tyewrvprea ipgsfengaw ngligmlhrq eadigaygfs vtkersevvn ftsaydeapy
      421 kflvpkpran ykylfldpft wdtwvavlfs llligpvlwg ihcaspyydy yglrnnkglf
      481 llqncewycf gaiiqqggih lpdaisgril vgfwwlfviv tlttysgnlv adltfpkirn
      541 pvdnvdnlva hrgymrwgaf kgqavfdllk tqergplkvl sdrmmtfepn hemwvldqvr
      601 mgymaligse vtmfhylgre lnrtgkcdfa vargevirdv kslavrvgfp fldrlnnelr
      661 rlvesglvmh wkrkhwprdn ectveskpqa gdirkitlyh mtgsfwvlgv gfassftalf
      721 iefvrrkrel tappghkppt vihtkspfft rteyggggkd glgdrfatdy ggrgirdssg
      781 fafsppnspf ryngqpnnrs dlipynypar ry
//
LOCUS       XP_077522385             197 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133284 [Amblyomma americanum].
ACCESSION   XP_077522385
VERSION     XP_077522385.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666259.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..197
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..197
                     /product="uncharacterized protein LOC144133284"
                     /calculated_mol_wt=21505
     Region          <4..38
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     Region          <73..>172
                     /region_name="PRK14959"
                     /note="DNA polymerase III subunits gamma and tau;
                     Provisional"
                     /db_xref="CDD:184923"
     CDS             1..197
                     /gene="LOC144133284"
                     /coded_by="XM_077666259.1:1..594"
                     /db_xref="GeneID:144133284"
ORIGIN      
        1 maaariqrwa hhlgayryrl qyapgrqmln adalsrlpqq spqadpsalp gpkennydll
       61 plllsevyar nygagsiwtp chvettsgar mvtvetsdgi vqrhvcqvrp rpeaprdnpa
      121 vttatthcsa pdqatqlqhp etvspdegis arrtpngtgs pdlpapdipv aplntgvaqq
      181 tlrrstrehk pvqrfhf
//
LOCUS       XP_077522397             198 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger X-chromosomal protein-like [Amblyomma americanum].
ACCESSION   XP_077522397
VERSION     XP_077522397.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666271.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..198
                     /product="zinc finger X-chromosomal protein-like"
                     /calculated_mol_wt=22857
     Region          2..20
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          30..50
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(30,33,46,50)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(35,37,39,41..42,45..46,49,63,65,69..70,73..74,77,91,
                     93,95,97..98,101..102)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          52..>112
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          58..78
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          70..93
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          86..102
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          115..131
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..198
                     /gene="LOC144133290"
                     /coded_by="XM_077666271.1:43..639"
                     /db_xref="GeneID:144133290"
ORIGIN      
        1 mcgdlfttqy kmemhtrwkh wrskkgihqc thctftstrk adiirherth vdkrnsvcnl
       61 cqkrfsrpdn lkvhmmihtr dkpyecsqcg krfrerahar rheevihsrr yplhcprcga
      121 gaedvtklrr htckqlgtte gkegfkrgrg rprlmadgta ptvrprntte gaplqggvkr
      181 grgqprkala qdaskprp
//
LOCUS       XP_077522438             303 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133316 [Amblyomma americanum].
ACCESSION   XP_077522438
VERSION     XP_077522438.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666312.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..303
                     /product="uncharacterized protein LOC144133316"
                     /calculated_mol_wt=32590
     Region          <151..297
                     /region_name="LGT"
                     /note="Prolipoprotein diacylglyceryl transferase; cl00478"
                     /db_xref="CDD:469786"
     CDS             1..303
                     /gene="LOC144133316"
                     /coded_by="XM_077666312.1:103..1014"
                     /db_xref="GeneID:144133316"
ORIGIN      
        1 miidpegqdv kvrlhwmlpg vddeeirtaf aafgkvtavk herwqvqgmt dkasttrtvl
       61 lkmkpgvtie diphqvsvag elpmvvvpcr pmqclrcrgt ghvwrdckip rctkctrfgh
      121 gdancvrtya svtvaavdee kaekqmdaae aeevskgaee tdkaavatvs spssaedtgt
      181 psdqgekpep pastaikmae dggsvgaskt evpqpvceed mddsdsrlva isapvkrhep
      241 arkekaneta asgeeppakt vharrpdfkp rpnfsaerrv aasspvrqgp gdrpddpgfk
      301 esv
//
LOCUS       XP_077522446             303 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133322 [Amblyomma americanum].
ACCESSION   XP_077522446
VERSION     XP_077522446.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666320.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..303
                     /product="uncharacterized protein LOC144133322"
                     /calculated_mol_wt=32590
     Region          <151..297
                     /region_name="LGT"
                     /note="Prolipoprotein diacylglyceryl transferase; cl00478"
                     /db_xref="CDD:469786"
     CDS             1..303
                     /gene="LOC144133322"
                     /coded_by="XM_077666320.1:103..1014"
                     /db_xref="GeneID:144133322"
ORIGIN      
        1 miidpegqdv kvrlhwmlpg vddeeirtaf aafgkvtavk herwqvqgmt dkasttrtvl
       61 lkmkpgvtie diphqvsvag elpmvvvpcr pmqclrcrgt ghvwrdckip rctkctrfgh
      121 gdancvrtya svtvaavdee kaekqmdaae aeevskgaee tdkaavatvs spssaedtgt
      181 psdqgekpep pastaikmae dggsvgaskt evpqpvceed mddsdsrlva isapvkrhep
      241 arkekaneta asgeeppakt vharrpdfkp rpnfsaerrv aasspvrqgp gdrpddpgfk
      301 esv
//
LOCUS       XP_077522460             232 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133329 [Amblyomma americanum].
ACCESSION   XP_077522460
VERSION     XP_077522460.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666334.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..232
                     /product="uncharacterized protein LOC144133329"
                     /calculated_mol_wt=25557
     CDS             1..232
                     /gene="LOC144133329"
                     /coded_by="XM_077666334.1:333..1031"
                     /db_xref="GeneID:144133329"
ORIGIN      
        1 mddfmrlagv vgerikchaf edghtqlvsl neycwsaigl yldlddiqew lddlnktadg
       61 hssiwtsedn gcppprlpph cypvavpcgs nllpgndrrp swcsclmpll skqppprrwk
      121 rwytassvtv lpcsstlhaa ptchlvttdg argvsclapl lskqtprhrk rrypasraml
      181 lpcsstlrsq pavwkrptal vvqlpdgpav qtalyvppet gqssvhrrta tl
//
LOCUS       XP_077522468             232 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133329 [Amblyomma americanum].
ACCESSION   XP_077522468
VERSION     XP_077522468.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666342.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..232
                     /product="uncharacterized protein LOC144133329"
                     /calculated_mol_wt=25557
     CDS             1..232
                     /gene="LOC144133329"
                     /coded_by="XM_077666342.1:304..1002"
                     /db_xref="GeneID:144133329"
ORIGIN      
        1 mddfmrlagv vgerikchaf edghtqlvsl neycwsaigl yldlddiqew lddlnktadg
       61 hssiwtsedn gcppprlpph cypvavpcgs nllpgndrrp swcsclmpll skqppprrwk
      121 rwytassvtv lpcsstlhaa ptchlvttdg argvsclapl lskqtprhrk rrypasraml
      181 lpcsstlrsq pavwkrptal vvqlpdgpav qtalyvppet gqssvhrrta tl
//
LOCUS       XP_077522480             152 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133342 [Amblyomma americanum].
ACCESSION   XP_077522480
VERSION     XP_077522480.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666354.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..152
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..152
                     /product="uncharacterized protein LOC144133342"
                     /calculated_mol_wt=16612
     CDS             1..152
                     /gene="LOC144133342"
                     /coded_by="XM_077666354.1:74..532"
                     /db_xref="GeneID:144133342"
ORIGIN      
        1 mddvmrlagv vrervkchaf edghaqqadg qssiwtsedn gcpsprlpph cypvavpcgs
       61 nllpgkgrrr lwrqlpdvsa vqtaplrrwk rwytapsatl lpcsstlrfq paawkrptal
      121 mvqlpdapav qtapsaplet vvysvlhhaa tl
//
LOCUS       XP_077522492             152 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133348 [Amblyomma americanum].
ACCESSION   XP_077522492
VERSION     XP_077522492.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666366.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..152
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..152
                     /product="uncharacterized protein LOC144133348"
                     /calculated_mol_wt=16612
     CDS             1..152
                     /gene="LOC144133348"
                     /coded_by="XM_077666366.1:128..586"
                     /db_xref="GeneID:144133348"
ORIGIN      
        1 mddvmrlagv vrervkchaf edghaqqadg qssiwtsedn gcpsprlpph cypvavpcgs
       61 nllpgkgrrr lwrqlpdvsa vqtaplrrwk rwytapsatl lpcsstlrfq paawkrptal
      121 mvqlpdapav qtapsaplet vvysvlhhaa tl
//
LOCUS       XP_077522523             703 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133354 [Amblyomma americanum].
ACCESSION   XP_077522523
VERSION     XP_077522523.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666397.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..703
                     /product="uncharacterized protein LOC144133354"
                     /calculated_mol_wt=78194
     Region          <168..396
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     CDS             1..703
                     /gene="LOC144133354"
                     /coded_by="XM_077666397.1:1..2112"
                     /db_xref="GeneID:144133354"
ORIGIN      
        1 merpsgsaqa lmrrdvselp glplctgter agckitahls klnaffwnar lelrklcgtp
       61 aklalvside qglplpnrkq irqgaalahr llkthpclaa ldintssfkg setllrdale
      121 dnasiksfki hfsglvrred fcsaipslmn lehlegstae dcpvalssai tealqraasl
      181 rilrvpnlrm kgsnaneffa altascclee lslhasmise aslayraefs ryvaatrtlt
      241 klsvdshnel rqntmkwile gllenrtvte vsltdfvidp esaavmtevl arnsilrrln
      301 ittaalgawe rlqvdvasta dcspwlhala engtleaial plasfepesw qllfralstk
      361 nslmtltisg lrsqrylwek asealissga ednvsfetti nvvqnreslr crgfsqiyaf
      421 ayrdtwvevd rtlvqisslt hitsahleiw lpgvdktmcm aiahyisata slrklqitvw
      481 lrnifreptk eccqaicgal rqnasitelr vlaklmddde velladavrg srnirrvhlt
      541 agepriavaf vrrlrfsied hrnlvnvtvd gcelsgghla tdlfvihdta rrnadavawa
      601 sdfvvgavyd rysacalerv arhpalqdel aeqlsvsrae atdlvrrglr rtqsmddfmr
      661 lagvvrervk chafedgraq ldslneycwi airryldlnd iqe
//
LOCUS       XP_077522539             358 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133355 [Amblyomma americanum].
ACCESSION   XP_077522539
VERSION     XP_077522539.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666413.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..358
                     /product="uncharacterized protein LOC144133355"
                     /calculated_mol_wt=39876
     Region          96..141
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          176..343
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..358
                     /gene="LOC144133355"
                     /coded_by="XM_077666413.1:265..1341"
                     /db_xref="GeneID:144133355"
ORIGIN      
        1 metetdlsmq nishlieeng rlkqrlasle eecsaakqka gelenkhtnh aiitkevvmq
       61 saktvkfytg fvstamftaf lqfvlsawkp scatwldaet qlimvlmrlr lglltqdlsc
      121 rfgisnatvs sifhawldvl aanmtkliiw psrqtlqanr paafqdpffd gvtgiidcte
      181 vfiqrpilmt arsqtyshyk rhntvkilvv ispsgaisfv skawggrtpd keitlrsgll
      241 dkvkegdvfl vdrgfrcgem faargatllm psqtknraql pgaevttsrk vssvrihver
      301 aiqrlkvfri fqtvlplnfv krtgdknlat idkvvvvcsa lvnlqtpiik nsqrrsas
//
LOCUS       XP_077522554             100 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133358 [Amblyomma americanum].
ACCESSION   XP_077522554
VERSION     XP_077522554.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666428.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..100
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..100
                     /product="uncharacterized protein LOC144133358"
                     /calculated_mol_wt=11335
     CDS             1..100
                     /gene="LOC144133358"
                     /coded_by="XM_077666428.1:105..407"
                     /db_xref="GeneID:144133358"
ORIGIN      
        1 melrvtkael iqrmskrkit amdlekpeps ttvaqihdqi stvrmramrr nskqssrghs
       61 psvihgqpwr hdcqqagsgg dqfilplawi llhhrtrisc
//
LOCUS       XP_077522577             399 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133371 [Amblyomma americanum].
ACCESSION   XP_077522577
VERSION     XP_077522577.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666451.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..399
                     /product="uncharacterized protein LOC144133371"
                     /calculated_mol_wt=44229
     Region          <62..>206
                     /region_name="PspC_subgroup_1"
                     /note="pneumococcal surface protein PspC, choline-binding
                     form; NF033838"
                     /db_xref="CDD:468201"
     CDS             1..399
                     /gene="LOC144133371"
                     /coded_by="XM_077666451.1:81..1280"
                     /db_xref="GeneID:144133371"
ORIGIN      
        1 myvlvryage drsyilqhtd vrdfvphdie dydpkkvydi fwagdsstcg gyydgkiihm
       61 tdteeemeqw rkrkearkca rktdipprkk arleeqakgv akknaerell ssrvgnspee
      121 sklahleslv rdlkaelerc kevigdkeke mknlrslnes lekalctkil qndhsqnenv
      181 saaidkvfwe gdsatqavns amhshslgti apvlpldval plaappvapp radvappqad
      241 vappradvap pradvtpavp parpmskapr kaaqdldltv gttnetgevh vgdghyidpg
      301 twerlltann dsafckqlav rlwdntvlse rsvtgtlsnn aiskgatkvf pplspakvas
      361 madsfwhflk lkgclgdeml krhkrlpryl aqkcsdlrr
//
LOCUS       XP_077522606             689 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 1-like [Amblyomma americanum].
ACCESSION   XP_077522606
VERSION     XP_077522606.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666480.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..689
                     /product="endothelin-converting enzyme 1-like"
                     /calculated_mol_wt=75931
     Region          137..685
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..689
                     /gene="LOC144133395"
                     /coded_by="XM_077666480.1:207..2276"
                     /db_xref="GeneID:144133395"
ORIGIN      
        1 mldgrlprts alfvrfrlpl sahelrsedt rgpwqrrrkg pseardrlsr rgpfhppapa
       61 agtaileatk mcdqggsgrr kwrcgvtgam lvglplallg latclgrrgt vgspgvcsta
      121 acrtaalaln vslsrnvapc edlqhfvcdg wprmerassr velimdyawt syvvphrkqs
      181 avdkvvalfq scvaalspgg gdrgtlrall aqlgltpsta slgsafravl vlssrhslhl
      241 lfavernatg itlravhppp aggppayvrr vtelalrddp elvgrdlslv eselaarpvp
      301 deqeertval evvadecgtl rdwltelpga nthtpvavqa pvavrwyanf frrhrsdpal
      361 rryvawhtar vlgsltqhdl frttlqnslg llepspalvk atclahaanl mplaynafvv
      421 rhfvtrrsrr avesmaeglr aaaaarvras rwmsdrpkam atakirrltw llprhrhelp
      481 vnrayshtgd lgltdflssy lnvtehsvhg dseeanqasv nvayspkqnt ivvtagmlnt
      541 pffeetlpaa fnyaglgqrm aaellhsvde egrwydergr krswwddata deyrnrlqcp
      601 kgggralles savrlafqaf rrthqhvllk gleiyspdqi ffisschkwc gregspcnma
      661 mrnleefata fkckpgqams phercpllv
//
LOCUS       XP_077522617             468 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133404 [Amblyomma americanum].
ACCESSION   XP_077522617
VERSION     XP_077522617.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666491.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..468
                     /product="uncharacterized protein LOC144133404"
                     /calculated_mol_wt=53442
     Region          22..58
                     /region_name="Beta-TrCP_D"
                     /note="D domain of beta-TrCP; cl26390"
                     /db_xref="CDD:474793"
     Region          58..108
                     /region_name="F-box_SF"
                     /note="F-box domain superfamily; cl45894"
                     /db_xref="CDD:459239"
     Site            order(70..74,77..78,81,92..94,96,99)
                     /site_type="other"
                     /note="F-box motif"
                     /db_xref="CDD:438852"
     Site            order(70,73,76..77,80..81,85,87..88,92..94,96)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438852"
     Region          183..461
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cd00200"
                     /db_xref="CDD:238121"
     Region          186..221
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Site            order(198,202,208..209,221..222,238,242,248..249,261..262,
                     277,282,288..289,305,320,325,331..332,344..345,361,365,
                     371..372,384..385,400,405,411..412,433..434,450,454,
                     460..461)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          227..261
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          266..303
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          310..343
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          349..383
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          389..432
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          438..460
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..468
                     /gene="LOC144133404"
                     /coded_by="XM_077666491.1:1..1407"
                     /db_xref="GeneID:144133404"
ORIGIN      
        1 mqwdgsrhaa sqadherclv sfdtwpeevq tqfveallar msqcqhsris tflmpmlqrd
       61 fisllpkkgl dhvaeailsy ldarslcaae avcrewrrvv adgglwrklm errvstdplw
      121 rglaerrgwv qylfkpppge phpdhmfyrr lypriiqdie siennwrcgr hnlqrincrs
      181 enskgvyclq ydvtkivsgl rdntikiwdr aslqcvkvlt ghtgavlclq yddkviisgs
      241 ydstvrvwdv ktgemvntli hhreavlhlr fnddmmvtcs kdhsiavwdm vapreinlrr
      301 vlvghraavn vvdfderyvv sasgdltikv wgtpncefvr tlnghtrgia clqyrdrlvv
      361 sgssdntirl wdiecgaclr vleghkelvr ctrfdtkriv sgaydgeikv wdlaavldpr
      421 apagtlclrt lvahsgrvfr lqfddfqivs ssgddtiliw dfmlalav
//
LOCUS       XP_077522629             490 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133411 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077522629
VERSION     XP_077522629.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666503.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..490
                     /product="uncharacterized protein LOC144133411 isoform X1"
                     /calculated_mol_wt=56100
     Region          22..58
                     /region_name="Beta-TrCP_D"
                     /note="D domain of beta-TrCP; cl26390"
                     /db_xref="CDD:474793"
     Region          58..108
                     /region_name="F-box_SF"
                     /note="F-box domain superfamily; cl45894"
                     /db_xref="CDD:459239"
     Site            order(70..74,77..78,81,92..94,96,99)
                     /site_type="other"
                     /note="F-box motif"
                     /db_xref="CDD:438852"
     Site            order(70,73,76..77,80..81,85,87..88,92..94,96)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438852"
     Region          183..483
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cd00200"
                     /db_xref="CDD:238121"
     Region          186..221
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Site            order(198,202,208..209,221..222,238,242,248..249,261..262,
                     277,282,288..289,305,320,325,331..332,344..345,361,365,
                     371..372,384..385,400,405,411..412,455..456,472,476,
                     482..483)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          227..261
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          266..303
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          310..343
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          349..383
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          389..427
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          433..482
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..490
                     /gene="LOC144133411"
                     /coded_by="XM_077666503.1:24..1496"
                     /db_xref="GeneID:144133411"
ORIGIN      
        1 mqwdgsrhaa sqadrerclv sfdtwpeevq tqfveellar mshcqhsris tflmpmrqrd
       61 fisllpkkgl dhvaeailsy ldarslcaae avcrewrrvv aegglwrklm errvstdplw
      121 rglaerrglv qylfkpppge phpdhmfyrr lypriiqdie siennwrfgr hnlqrincrs
      181 enskgvyclq yddtkivsgl rdntikiwdr aslqcvkvlt ghtgavlclq yddkviisgs
      241 ydstvrvwdv ktgemvntli hhreavlhlr fkydmmvtcs kdhsiavwdm vtpreinlrr
      301 vlvghraavn vvdfderyvv sasadrtikv wgtpncefvr tlnghkrgia clqyrdrlvv
      361 sgssdntirl wdiecgaclr vlegheelvr cirfdtkriv sgaydgeikv wdlaaaldpr
      421 apagtlclrt lvvravcwcs qpclfliasm sasqahsgrv frlqfddfqi vsssgddtil
      481 iwdfmlapav
//
LOCUS       XP_077522634             468 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133411 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077522634
VERSION     XP_077522634.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666508.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..468
                     /product="uncharacterized protein LOC144133411 isoform X2"
                     /calculated_mol_wt=53717
     Region          22..58
                     /region_name="Beta-TrCP_D"
                     /note="D domain of beta-TrCP; cl26390"
                     /db_xref="CDD:474793"
     Region          58..108
                     /region_name="F-box_SF"
                     /note="F-box domain superfamily; cl45894"
                     /db_xref="CDD:459239"
     Site            order(70..74,77..78,81,92..94,96,99)
                     /site_type="other"
                     /note="F-box motif"
                     /db_xref="CDD:438852"
     Site            order(70,73,76..77,80..81,85,87..88,92..94,96)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438852"
     Region          183..461
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cd00200"
                     /db_xref="CDD:238121"
     Region          186..221
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Site            order(198,202,208..209,221..222,238,242,248..249,261..262,
                     277,282,288..289,305,320,325,331..332,344..345,361,365,
                     371..372,384..385,400,405,411..412,433..434,450,454,
                     460..461)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          227..261
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          266..303
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          310..343
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          349..383
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          389..432
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          438..460
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..468
                     /gene="LOC144133411"
                     /coded_by="XM_077666508.1:24..1430"
                     /db_xref="GeneID:144133411"
ORIGIN      
        1 mqwdgsrhaa sqadrerclv sfdtwpeevq tqfveellar mshcqhsris tflmpmrqrd
       61 fisllpkkgl dhvaeailsy ldarslcaae avcrewrrvv aegglwrklm errvstdplw
      121 rglaerrglv qylfkpppge phpdhmfyrr lypriiqdie siennwrfgr hnlqrincrs
      181 enskgvyclq yddtkivsgl rdntikiwdr aslqcvkvlt ghtgavlclq yddkviisgs
      241 ydstvrvwdv ktgemvntli hhreavlhlr fkydmmvtcs kdhsiavwdm vtpreinlrr
      301 vlvghraavn vvdfderyvv sasadrtikv wgtpncefvr tlnghkrgia clqyrdrlvv
      361 sgssdntirl wdiecgaclr vlegheelvr cirfdtkriv sgaydgeikv wdlaaaldpr
      421 apagtlclrt lvahsgrvfr lqfddfqivs ssgddtiliw dfmlapav
//
LOCUS       XP_077522656             237 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133433 [Amblyomma americanum].
ACCESSION   XP_077522656
VERSION     XP_077522656.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666530.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..237
                     /product="uncharacterized protein LOC144133433"
                     /calculated_mol_wt=26103
     Region          43..201
                     /region_name="Grp7_allergen"
                     /note="Group 7 allergen; pfam16984"
                     /db_xref="CDD:319052"
     CDS             1..237
                     /gene="LOC144133433"
                     /coded_by="XM_077666530.1:217..930"
                     /db_xref="GeneID:144133433"
ORIGIN      
        1 mcqasllwsl lafctssrll laqltgnpsm panqnvyvrn pkesnfymdr illtdmiaqv
       61 rfldldpfhl ppfetdfkds ilgfpvkgha kfvngtlrgl srvnrfgdcs apswvngnit
      121 vtcnlliddl divydarvky glapevrvsv esrvgtcyah meataapgka avlrsfqitg
      181 lgdldtkwsg lgplnpylrt indgyrania gvvystfyss ykvaldrsls repipkp
//
LOCUS       XP_077522732             432 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133484 [Amblyomma americanum].
ACCESSION   XP_077522732
VERSION     XP_077522732.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666606.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..432
                     /product="uncharacterized protein LOC144133484"
                     /calculated_mol_wt=47909
     CDS             1..432
                     /gene="LOC144133484"
                     /coded_by="XM_077666606.1:169..1467"
                     /db_xref="GeneID:144133484"
ORIGIN      
        1 msqelprqap psselapiiq araeqpalew prrdiavsde eamvcvdaee dqegatgsla
       61 psshcgiqrh egkvvkephe ltphsgaevv hdrspivars itvaawtens npsginsini
      121 eahtepsels klnpdttaap rhllmqcgep ssleqqlddr pkrlhssqss falntghmaq
      181 sslepeewsr rwktflirrp laeekctqas idadcrehcd svckhrqqre lfrceegisf
      241 ddacgkiqpe ewsrrwkrfl irrnqledel qlsndleskr mipltpavne gesmqlssdp
      301 selqeeeeav anstgdvqwl yevqplhefe peaevsaawd pelelsfleh qdpgyprsst
      361 pkrlsslsil dlgqpelstt rcsvldstls fldlpsppsy lalppiaars apksdvgetd
      421 tgaaaaekpa qe
//
LOCUS       XP_077522745             368 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133488 [Amblyomma americanum].
ACCESSION   XP_077522745
VERSION     XP_077522745.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666619.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..368
                     /product="uncharacterized protein LOC144133488"
                     /calculated_mol_wt=40251
     CDS             1..368
                     /gene="LOC144133488"
                     /coded_by="XM_077666619.1:278..1384"
                     /db_xref="GeneID:144133488"
ORIGIN      
        1 msqelprqap psselapiiq araeqaalew prrdiavsde eamvcvdaee dqdgatgsla
       61 psshcgiqrh egqvvkephe ltphsgaevv hdrspivars itvaartens npsginsini
      121 eahtepsqls klnpdttaap rhllmqcgep srleqqlddr pkrlhssqss falntghmaq
      181 sslepeewsr rwkrflirwn qledelqlsn dleskrmipl tpavnegesm qlssdpsqlq
      241 edeeavanst gdvqwlyevq plhefepeae vsaawdpele lsflehqdpg yprsstpkpl
      301 aslsildlgq pelsttrcsv ldstlsfldl psppsylalp piavrsapks dvgetdtgaa
      361 aaekpaqe
//
LOCUS       XP_077522777              64 aa            linear   INV 25-NOV-2025
DEFINITION  fibroblast growth factor receptor 2-like [Amblyomma americanum].
ACCESSION   XP_077522777
VERSION     XP_077522777.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666651.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..64
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..64
                     /product="fibroblast growth factor receptor 2-like"
                     /calculated_mol_wt=7136
     Region          <1..46
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     CDS             1..64
                     /gene="LOC144133505"
                     /coded_by="XM_077666651.1:6..200"
                     /db_xref="GeneID:144133505"
ORIGIN      
        1 mrecwnqspy qrptftelve dldriltfat dqeyldlsip vldtppssse resslsiaap
       61 hvav
//
LOCUS       XP_077522808             572 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133524 [Amblyomma americanum].
ACCESSION   XP_077522808
VERSION     XP_077522808.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666682.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..572
                     /product="uncharacterized protein LOC144133524"
                     /calculated_mol_wt=64232
     CDS             1..572
                     /gene="LOC144133524"
                     /coded_by="XM_077666682.1:90..1808"
                     /db_xref="GeneID:144133524"
ORIGIN      
        1 mgdvvlqgdr daseltkmld nrdpqrvmtm kltnciladp dalerqisrc trlqnlhcls
       61 trlkpsglvr lllgplqqlr hlefsivvkd ddvaaevanv edmigdqehr esslvylyve
      121 lvnasnfall lvmlplfpml nelhvhvlrg llepaftkcs yilrtyeria tfkmtsevas
      181 rsehmpgttp smvraaracg nvmqlrshtr cnylrlsdla egpesvhsid pvlliayydd
      241 atpshfaaas gpgrwqnvha vqrfcmllfh tgntrfypha ntsyfqplcs ffgrftslae
      301 lninsfhcag didmtavlat arfpamrals ispcalqhsg avsrlselse fledldirfk
      361 sggyynrcat cltalvleeq pmadlqarte lmrftlayvp evssyqflrk ltvaevrlie
      421 spctenrsyt glnvllsqnl rlrrlvivdk hlavdqpnlq rivgnvvqlr llvlcsedti
      481 dedpvphwlg vadllanlev mhvhfrsang smermtwlrr petdaalqgv vqadpdvqdg
      541 gwllhnrpcv vcstetfigl vkphnrgtrt hf
//
LOCUS       XP_077522833             119 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133537 [Amblyomma americanum].
ACCESSION   XP_077522833
VERSION     XP_077522833.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666707.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..119
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..119
                     /product="uncharacterized protein LOC144133537"
                     /calculated_mol_wt=13704
     CDS             1..119
                     /gene="LOC144133537"
                     /coded_by="XM_077666707.1:59..418"
                     /db_xref="GeneID:144133537"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedrrr rhlnrrltat mkrlsgvhvl
       61 nhehrfldas gepmlslfat dryhvarrqg idpnaplfic sgksrvkvat iilplqsal
//
LOCUS       XP_077522840             119 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133537 [Amblyomma americanum].
ACCESSION   XP_077522840
VERSION     XP_077522840.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666714.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..119
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..119
                     /product="uncharacterized protein LOC144133537"
                     /calculated_mol_wt=13704
     CDS             1..119
                     /gene="LOC144133537"
                     /coded_by="XM_077666714.1:59..418"
                     /db_xref="GeneID:144133537"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedrrr rhlnrrltat mkrlsgvhvl
       61 nhehrfldas gepmlslfat dryhvarrqg idpnaplfic sgksrvkvat iilplqsal
//
LOCUS       XP_077522923             216 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133609 [Amblyomma americanum].
ACCESSION   XP_077522923
VERSION     XP_077522923.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666797.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..216
                     /product="uncharacterized protein LOC144133609"
                     /calculated_mol_wt=23322
     CDS             1..216
                     /gene="LOC144133609"
                     /coded_by="XM_077666797.1:621..1271"
                     /db_xref="GeneID:144133609"
ORIGIN      
        1 matriinefd sdgayqppve slpvvrllqp midgpddmef dyaddlreda shagqsqppa
       61 atanesgapa aapegneias pivqpsssts niaplasgva trctpaaala sgdsskapkg
      121 rmallektld denevragll reehtlrlkl lqedhdsilh eratkhkfle qvqalelqkl
      181 kneilkqeae leilqiekki keqqlckalc sktpqg
//
LOCUS       XP_077522933             147 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133627 [Amblyomma americanum].
ACCESSION   XP_077522933
VERSION     XP_077522933.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666807.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..147
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..147
                     /product="uncharacterized protein LOC144133627"
                     /calculated_mol_wt=15840
     CDS             1..147
                     /gene="LOC144133627"
                     /coded_by="XM_077666807.1:118..561"
                     /db_xref="GeneID:144133627"
ORIGIN      
        1 mirrisssdd sdevdlenfd vpeppfrsqs cprgtddatr gdrplsssas qlrcsdspla
       61 casssqrssp lstpasdsqh sgkqsfqpnq mqssvhaish iellfaqegp qpadvfyqnt
      121 sagtylevlk fvcvnlclif ffatsac
//
LOCUS       XP_077522938             186 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133636 [Amblyomma americanum].
ACCESSION   XP_077522938
VERSION     XP_077522938.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666812.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..186
                     /product="uncharacterized protein LOC144133636"
                     /calculated_mol_wt=20711
     Region          64..140
                     /region_name="DUF4806"
                     /note="Domain of unknown function (DUF4806); pfam16064"
                     /db_xref="CDD:465001"
     CDS             1..186
                     /gene="LOC144133636"
                     /coded_by="XM_077666812.1:250..810"
                     /db_xref="GeneID:144133636"
ORIGIN      
        1 mvdtmsyqhh rgrsgslval ikkeissstr avlreiefvk rqndliltll kqqvtaeehc
       61 deptlpllqm aqvdelehrl ansdharkhl ckmlsriggs tthnttanim kylfhdsvgv
      121 efslhgqkgk kafnstqlfh viieavqksp nttsatqaei cgaimawlkn crarlttask
      181 rtttse
//
LOCUS       XP_077522941             893 aa            linear   INV 25-NOV-2025
DEFINITION  Bardet-Biedl syndrome 9 [Amblyomma americanum].
ACCESSION   XP_077522941
VERSION     XP_077522941.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666815.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..893
                     /product="Bardet-Biedl syndrome 9"
                     /calculated_mol_wt=98273
     Region          1..417
                     /region_name="PHTB1_N"
                     /note="PTHB1 N-terminus; pfam14727"
                     /db_xref="CDD:464284"
     Region          447..812
                     /region_name="PHTB1_C"
                     /note="PTHB1 C-terminus; pfam14728"
                     /db_xref="CDD:464285"
     CDS             1..893
                     /gene="BBS9"
                     /coded_by="XM_077666815.1:206..2887"
                     /db_xref="GeneID:144133645"
ORIGIN      
        1 mslfkardww tatcgtdeaf daaclcigna tndpskseqi lvgshagvlr vfqptcaqke
       61 dgsfegfrpe hvllefqmtq ailqvllgqf vsgsdklfia alhprrisvh sltgdageae
      121 pgtqyklclm yehslsapsf ccvagsfggv kgqdflcvqg vdgsltffeq enfafarylp
      181 efllpgplay isktdsfvtv ntafhlenfr fqtlavatea skkpsddtng lqkgkrvvad
      241 wsvsigeeal dikvasfpnc ptlilvlgeh tffafkpsge lhflkmldft aacffpypss
      301 tegslmllma tqrstllvyh dtvlkwaaql ssapialart tiqsmqgaiv rlseqgqlsc
      361 sylgtdpcls vatppesrey slreveqels rlesaikssg evfpgrkkte tdlkvkvfag
      421 pmsevpcsde nvaltedgna fipalivkvh lesptplhdv rimvdvakpl valngdvtlt
      481 slldhhqmaf tvkqagpmlp ssldlkvval yqsssgasqv vqasmhlpla lvarpcvaer
      541 myehkltiet nkpsvpsnel fpdftcdits qtigiqflhg pqasilvsks sqkyriqsdn
      601 faalwipvle llrrlhqyyk rsandeslkc sygsslplqe yfsiiddyfk irqealevee
      661 llsqraaqfr vvqkcvltkl kdktptplnn fdsllevtqr ellslgkhav eitneyqsml
      721 sklgsasqll vlllklktqm spedhkilqd ifhvdleasi tegweekvea aichllhasq
      781 sdeelppvpk elaplkdvtr lkqliaiafe kvldglrlsq ndqtgrkged qskdgdnkep
      841 kkemfaldsa stmspipgps tskalpdegn lqewtadsqe sessnnnlle esp
//
LOCUS       XP_077522956             176 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133677 [Amblyomma americanum].
ACCESSION   XP_077522956
VERSION     XP_077522956.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666830.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..176
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..176
                     /product="uncharacterized protein LOC144133677"
                     /calculated_mol_wt=19034
     CDS             1..176
                     /gene="LOC144133677"
                     /coded_by="XM_077666830.1:260..790"
                     /db_xref="GeneID:144133677"
ORIGIN      
        1 msapdydaqq pgqhqelvvl radaghitia rnsgvvvpit qvhhlaprrv gelmgaevlq
       61 qlfrdlvqaa calprgpvfd acgaftssgl hgvgedeeel vhlllalgyi glcrltcdra
      121 sggapagrlp fvagvrryma alsrqchrrd rnfcvdtvgl elwhvrrgim famntq
//
LOCUS       XP_077522961             295 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133686 [Amblyomma americanum].
ACCESSION   XP_077522961
VERSION     XP_077522961.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666835.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..295
                     /product="uncharacterized protein LOC144133686"
                     /calculated_mol_wt=33449
     CDS             1..295
                     /gene="LOC144133686"
                     /coded_by="XM_077666835.1:76..963"
                     /db_xref="GeneID:144133686"
ORIGIN      
        1 madknptvvp earvrtkaep qtmfkellak tarnarlldr rkntpqpshh sgvlhkptts
       61 shrklpesrl garkplkptm vwrspnaspr llaaspapls plaeespvql yprpslrspr
      121 hdlnatfnkl rawrspqedr prtsynsspr lpqydirrdh ndfdstrnye ssgsnqsvpl
      181 yrprhlrppr rrawdsplaa errdhlqpas iavsptrdfq rarrcwneap segllvhhal
      241 haeghfippr gaisspgvrk rpgdnmirrf gshaawvtde yakhkqqgpg yygsp
//
LOCUS       XP_077522980             104 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133716 [Amblyomma americanum].
ACCESSION   XP_077522980
VERSION     XP_077522980.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666854.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..104
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..104
                     /product="uncharacterized protein LOC144133716"
                     /calculated_mol_wt=11307
     CDS             1..104
                     /gene="LOC144133716"
                     /coded_by="XM_077666854.1:476..790"
                     /db_xref="GeneID:144133716"
ORIGIN      
        1 mrpggrhima vseplmrlqe kipeyaeqgv dvkvhsegga gswavllvtp imrraqqles
       61 ssevifvdst ascdstrssv tiyadsnisw chthsshcaq qsdy
//
LOCUS       XP_077522988             143 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133731 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077522988
VERSION     XP_077522988.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666862.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..143
                     /product="uncharacterized protein LOC144133731 isoform X1"
                     /calculated_mol_wt=15794
     CDS             1..143
                     /gene="LOC144133731"
                     /coded_by="XM_077666862.1:374..805"
                     /db_xref="GeneID:144133731"
ORIGIN      
        1 metpcvappt srtlhtlaed gsfrgasrir tqetpyfklr mtclvilspt tlklswqlts
       61 prhltefdtm psygncrlys cpadppprgq haisswedwe vllmsadpts qlaavtraad
      121 visrhdllda vrdravaqgp swv
//
LOCUS       XP_077522990              96 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133731 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077522990
VERSION     XP_077522990.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666864.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..96
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..96
                     /product="uncharacterized protein LOC144133731 isoform X2"
                     /calculated_mol_wt=10286
     CDS             1..96
                     /gene="LOC144133731"
                     /coded_by="XM_077666864.1:356..646"
                     /db_xref="GeneID:144133731"
ORIGIN      
        1 metpcvappt srtlhtlaed gsfrgasrir tqdcpadppp rgqhaisswe dwevllmsad
       61 ptsqlaavtr aadvisrhdl ldavrdrava qgpswv
//
LOCUS       XP_077523001             159 aa            linear   INV 25-NOV-2025
DEFINITION  natterin-4-like [Amblyomma americanum].
ACCESSION   XP_077523001
VERSION     XP_077523001.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666875.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..159
                     /product="natterin-4-like"
                     /calculated_mol_wt=17074
     Region          35..143
                     /region_name="DUF3421"
                     /note="Protein of unknown function (DUF3421); pfam11901"
                     /db_xref="CDD:463390"
     CDS             1..159
                     /gene="LOC144133754"
                     /coded_by="XM_077666875.1:24..503"
                     /db_xref="GeneID:144133754"
ORIGIN      
        1 manyglsqyr ppchwvlchg esippnavpg gedkggramf varavhegdv ipgkvvpsrg
       61 fcyvpykkee hcykdyqvlv sdgaslawlp ssggdvpsga iqggvtssge plyigrarhe
      121 gnltvgkfhp shktvyvpfg gveygysqye ilvcetinf
//
LOCUS       XP_077523030             305 aa            linear   INV 25-NOV-2025
DEFINITION  translation initiation factor eIF2B subunit alpha-like [Amblyomma
            americanum].
ACCESSION   XP_077523030
VERSION     XP_077523030.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666904.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..305
                     /product="translation initiation factor eIF2B subunit
                     alpha-like"
                     /calculated_mol_wt=33580
     Region          26..293
                     /region_name="IF-2B"
                     /note="Initiation factor 2 subunit family; pfam01008"
                     /db_xref="CDD:395798"
     CDS             1..305
                     /gene="LOC144133810"
                     /coded_by="XM_077666904.1:108..1025"
                     /db_xref="GeneID:144133810"
ORIGIN      
        1 megvnadsin skleamlqnn sdlspavasl qlllefiekd dyttiqgmkd nlqhvisdlc
       61 takcsitcvr sacelfvrfa tltsldgaid eckenmstrg kaflekmraa rskiaalaap
      121 fvqdrttilt hsfsrvvrdt lfaaaddhkh fhvfvtesap dysgrqlyda leergisvtl
      181 ivdaavgyil ekvdmvllga egvvesggii nkigtytmam cakekniply vlaesfkfar
      241 qyplnqrdlp neqkyasdkl ngksdlskeh pmvdytppay itllftdlgi ltpsavsdel
      301 iklyl
//
LOCUS       XP_077523052             185 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133846 [Amblyomma americanum].
ACCESSION   XP_077523052
VERSION     XP_077523052.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666926.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..185
                     /product="uncharacterized protein LOC144133846"
                     /calculated_mol_wt=21626
     Region          10..101
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     CDS             1..185
                     /gene="LOC144133846"
                     /coded_by="XM_077666926.1:684..1241"
                     /db_xref="GeneID:144133846"
ORIGIN      
        1 mdkatfneal iteverrrvl wdvrdrdykn nmkrdqawra iavalsvdvt qvmkrwkslr
       61 dtfmkkrkev taiksgagad svvnvrwphf nqllflldtl eypetrgnli ktwtcskqra
      121 qsasrledll smklkplwml ptspqlhrkm eldfpapyqr hhqpaahhla hkkgrgamir
      181 lskrk
//
LOCUS       XP_077523058             352 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133856 [Amblyomma americanum].
ACCESSION   XP_077523058
VERSION     XP_077523058.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666932.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..352
                     /product="uncharacterized protein LOC144133856"
                     /calculated_mol_wt=37868
     CDS             1..352
                     /gene="LOC144133856"
                     /coded_by="XM_077666932.1:570..1628"
                     /db_xref="GeneID:144133856"
ORIGIN      
        1 mhsrraplqn tkmameqkes pspvtdqpwh gsepasrppe hpgilktpag lleppkphpa
       61 ktlrfqaepg rteegqatpq agcsgradet wtvnsdssds setsvgsatp sgsqsdsssp
      121 grptsgplrp rassapgtds kakvsswlpk rmrryfaalt rdkqgsqgkg ksspntatng
      181 rnrerrrshp shrrrtgvaa grytgppsqd dmrwrvalsr yalltllgsl tflcfviasl
      241 lfaraggdal hdkpqtqddg vidgrrpaaw ralrtsaqsn alpahelatk egegepplpp
      301 raatepgelv psigetplig rdaeqeeers siedtgtssl vpvrrhrdws kn
//
LOCUS       XP_077523063             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133867 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077523063
VERSION     XP_077523063.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666937.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144133867 isoform X1"
                     /calculated_mol_wt=14731
     CDS             1..133
                     /gene="LOC144133867"
                     /coded_by="XM_077666937.1:25..426"
                     /db_xref="GeneID:144133867"
ORIGIN      
        1 mskiflelgf ivseevrqqr dgysgaletc lpdiplydvl seqrccrswk qngagkctvc
       61 ghrgsnpqqv latlarggcv trrtadsrsc kpatwqdrec vtsanslqmp rnlkegshpl
      121 ddpicatrrp hrr
//
LOCUS       XP_077523069             119 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133867 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077523069
VERSION     XP_077523069.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666943.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..119
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..119
                     /product="uncharacterized protein LOC144133867 isoform X2"
                     /calculated_mol_wt=12778
     CDS             1..119
                     /gene="LOC144133867"
                     /coded_by="XM_077666943.1:86..445"
                     /db_xref="GeneID:144133867"
ORIGIN      
        1 mdtaahlatt dggrknhavd assgskpnkr ccrswkqnga gkctvcghrg snpqqvlatl
       61 arggcvtrrt adsrsckpat wqdrecvtsa nslqmprnlk egshplddpi catrrphrr
//
LOCUS       XP_077523076              82 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133867 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077523076
VERSION     XP_077523076.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666950.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..82
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..82
                     /product="uncharacterized protein LOC144133867 isoform X3"
                     /calculated_mol_wt=9040
     CDS             1..82
                     /gene="LOC144133867"
                     /coded_by="XM_077666950.1:25..273"
                     /db_xref="GeneID:144133867"
ORIGIN      
        1 mskiflelgf ivseevrqqr dgysgalall pqletkrswk myrvrtprlk sptgtsnaga
       61 rrmcdkedsg qqimqaghla gs
//
LOCUS       XP_077523092             181 aa            linear   INV 25-NOV-2025
DEFINITION  natterin-4-like [Amblyomma americanum].
ACCESSION   XP_077523092
VERSION     XP_077523092.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666966.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="natterin-4-like"
                     /calculated_mol_wt=20001
     Region          57..168
                     /region_name="DUF3421"
                     /note="Protein of unknown function (DUF3421); pfam11901"
                     /db_xref="CDD:463390"
     CDS             1..181
                     /gene="LOC144133905"
                     /coded_by="XM_077666966.1:36..581"
                     /db_xref="GeneID:144133905"
ORIGIN      
        1 mdhstrelsl cerrthsqyc samanygssm yrppchwvlc hkdhiphnav aggddkegev
       61 iyigravhng dvipgkvvrs mkccyipygg vepyyydyqa lvsdgaslaw lpasngavps
      121 gaiqggvtas geplyiarar hdgmlligkv hpswkcayip fhkkeykyte yqvlvcktin
      181 f
//
LOCUS       XP_077523103             159 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133925 [Amblyomma americanum].
ACCESSION   XP_077523103
VERSION     XP_077523103.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666977.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..159
                     /product="uncharacterized protein LOC144133925"
                     /calculated_mol_wt=14119
     CDS             1..159
                     /gene="LOC144133925"
                     /coded_by="XM_077666977.1:102..581"
                     /db_xref="GeneID:144133925"
ORIGIN      
        1 mrpavalall lvacllleae atkkklkkli llkklmkkgk iiiplpipip kkggggggam
       61 mhlksllgsl gggggggghg gggggghggg gggghggggg gmtevwkvel issggggghg
      121 gggghggggg ghggggggwm gggggggggw dmggggggw
//
LOCUS       XP_077523112             423 aa            linear   INV 25-NOV-2025
DEFINITION  gustatory receptor 58b [Amblyomma americanum].
ACCESSION   XP_077523112
VERSION     XP_077523112.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666986.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..423
                     /product="gustatory receptor 58b"
                     /calculated_mol_wt=48404
     CDS             1..423
                     /gene="Gr58b"
                     /coded_by="XM_077666986.1:1..1272"
                     /db_xref="GeneID:144133934"
ORIGIN      
        1 mldrwqialp ksedakhapa frvvdsdlar lksrrrvhpc aakddkyvvw raqelvipgl
       61 lmcavampgp flqfmsarnk aimrlyqcll yvgfsayeay rlkefvedym rsektslicf
      121 fhslvntflf pfiyayvarr sasldlllar wraesgdvsf skhrclakhr lwagfylaat
      181 vllvvgfhtl ntvrvcyeis wrytnrtlpi ksvmflgkta hhyvlqtmyc gleslafslm
      241 fllwmlsshl taqvrdipll ttaevtlvre kyrslcvvie atahflnsll flfffrtafd
      301 fmssvifysi qdgretklwv layeglltli nvfqntaiae msgklslemn salyevskvp
      361 avpeayndll lflqvykerp eamagcgvlr vdralmfkls gstvtivlil fqldpnlsnk
      421 vsf
//
LOCUS       XP_077523118             211 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133943 [Amblyomma americanum].
ACCESSION   XP_077523118
VERSION     XP_077523118.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666992.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..211
                     /product="uncharacterized protein LOC144133943"
                     /calculated_mol_wt=23806
     Region          70..90
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(70,73,86,90)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          98..118
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(98,101,114,118)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(103,105,107,109..110,113..114,117,131,133,137..138,
                     141..142,145,159,161,163,165..166,169..170,173)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          126..146
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          150..>211
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          154..174
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          182..199
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..211
                     /gene="LOC144133943"
                     /coded_by="XM_077666992.1:1533..2168"
                     /db_xref="GeneID:144133943"
ORIGIN      
        1 mwlccrwvsd prayvppypp yhqphhqvma ppvdarfmgt pqpvrqavpv aaaasaagpp
       61 aldagtrywc elcgqafvhr pallshwrtt hgicrfvcth cgvafqqrsq ltkhtrthtg
      121 erpfvcevcq srftqkghlv ehvrthtger pyrcdicqam ftqkahlvgh trrhtgerpf
      181 qcpacpkafa tssglnqhry anhrnpendn d
//
LOCUS       XP_077523123             228 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133952 [Amblyomma americanum].
ACCESSION   XP_077523123
VERSION     XP_077523123.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077666997.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..228
                     /product="uncharacterized protein LOC144133952"
                     /calculated_mol_wt=24889
     Region          27..199
                     /region_name="Grp7_allergen"
                     /note="Group 7 allergen; pfam16984"
                     /db_xref="CDD:319052"
     CDS             1..228
                     /gene="LOC144133952"
                     /coded_by="XM_077666997.1:230..916"
                     /db_xref="GeneID:144133952"
ORIGIN      
        1 mrrcqevvll tsllfravla qddvalaney adrvlasvrn drelvaavdp lqvpdfgens
       61 fqitggqvlg lsafyrsgns svqlsqdsai vttpaavndv fftgrhrasr fgltitgdie
      121 aavnrvhtli vyrapikgge arleyfqvdr mdgmkvtrit gvssafnwll rivanrvarh
      181 fqgrieaalq devskfistq vdksrfpals gngsrfsget faypgapf
//
LOCUS       XP_077523131             795 aa            linear   INV 25-NOV-2025
DEFINITION  beta-scruin-like [Amblyomma americanum].
ACCESSION   XP_077523131
VERSION     XP_077523131.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667005.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..795
                     /product="beta-scruin-like"
                     /calculated_mol_wt=87890
     Region          243..541
                     /region_name="NanM"
                     /note="N-acetylneuraminic acid mutarotase [Cell
                     wall/membrane/envelope biogenesis]; COG3055"
                     /db_xref="CDD:442289"
     Region          303..355
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          359..404
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          407..456
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          458..506
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          509..554
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     CDS             1..795
                     /gene="LOC144133960"
                     /coded_by="XM_077667005.1:7..2394"
                     /db_xref="GeneID:144133960"
ORIGIN      
        1 mgnrmseekg rrtevgldkc rthgpyfvrt idrrtsentg rrtevgrdqc rapakhfaet
       61 mdrpisadtt rltevgrrrr epsqimtafy rgqkkilwed tctrhqsfpr neaehpekvl
      121 trgswplmge rgignirpqq skwissahgg sgdqflswad pqhllsaasr prsawrlsam
      181 qlentvsgqk kselhachss lwlaasqqsd pdfwsplcki lppmtprtvd erneilgsff
      241 dvsfygdlat alqltyngpc hnlmliggis ptkladdahg aamlsygpgh drwrpygfma
      301 eprcdrtvvi vrnndvmlag gldplcvtts ghmqpsnkaf lfssrwkrri elpamirera
      361 fhaavgwhgr mmvfggfhrt grmlssaevy ffntnqslli rpmsmalmgm agapidtpvy
      421 iiggtscgpd ghtlqasiyi fdsstdswtk laammekraf fsalavqrdc wlaggvmqlr
      481 pkvkcttcid vlrlasstmq rrsvmpsprh svcaektgce vylfggqden nrplgdviml
      541 dsarrsfmqc eplprilags atavmpedgl dwlrlrsiwp gkkatpnire raatviqkay
      601 rnyrrgerlq prlkntwppp arstetlesh wdangaeasg atqylrqqtl renmdpnlgm
      661 vgrmdgsflk tnktatappd vlaptthptp qvhpvllssp ahpaslvpaa lkaspadtap
      721 laikappapp alpalptsta hptlppppal ppppahpaha adqepsvlpa ppdsppppal
      781 rrsqrirrkp drwgy
//
LOCUS       XP_077523140             303 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133976 [Amblyomma americanum].
ACCESSION   XP_077523140
VERSION     XP_077523140.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667014.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..303
                     /product="uncharacterized protein LOC144133976"
                     /calculated_mol_wt=34632
     Region          49..96
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          126..285
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..303
                     /gene="LOC144133976"
                     /coded_by="XM_077667014.1:408..1319"
                     /db_xref="GeneID:144133976"
ORIGIN      
        1 mlylenmktp qdmlyytglp svpvfnhils fvvernpkla rsekqnlrsl eeqlfmvlvr
       61 lrtgmatkei srnfavsmpt fsriftewil mldkdlaait tfpslaevqq hmpshfrqyp
      121 ntriildtte vriqkpsgln aqrytfsnyk fantmeclvg atrdcyisfv splfgggtsd
      181 rsiveetgvl dllepgdgvm adkgfkvtdl lpphvdlyms pfripgkrqm sandvrgtkq
      241 vasarvhier virrikeyhi ldkpfpinml diadqifatc arlsnfrrpl lntkrdeeqs
      301 ese
//
LOCUS       XP_077523144             538 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133986 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077523144
VERSION     XP_077523144.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667018.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..538
                     /product="uncharacterized protein LOC144133986 isoform X1"
                     /calculated_mol_wt=59610
     Region          13..98
                     /region_name="THAP"
                     /note="The THAP domain is a putative DNA-binding domain
                     (DBD) and probably also binds a zinc ion; smart00980"
                     /db_xref="CDD:214951"
     Region          289..340
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          370..530
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..538
                     /gene="LOC144133986"
                     /coded_by="XM_077667018.1:126..1742"
                     /db_xref="GeneID:144133986"
ORIGIN      
        1 maeaalpkrg pkyccvvgcr nsaentkgld ppvklyrfpg kwyekerlqa winavrrvsp
       61 dgtvwlpkeh tricsrhfvg nckidisqhp ayiptifppi yrkkapdrwq rrlnqaissr
      121 qlgagsenps rstddeatmh kpgtsitdcr hqdihmmdls daplemsaav astistsdlh
      181 psmsvcgtge shfiakrdaa sqtkasvaqg mltillsatn gtdastqvvh temsdkvast
      241 dgnwkkncgf qgfqslkeke kalqdlcgvt lqvfclllnl lpppkyrrnt msnedrlclf
      301 laklrlgity stlgvifsva pstastvfrk tlgilsialk dwvfmpsrra iklslpapfk
      361 enypnctlii dcteirteap snpdcqhily shykggytlk fligiipngm isfvskvygg
      421 rhtdsfitqd sgflelvrpg dlilsdkgfp sirttvegkg avllmppfns tggqmsdddm
      481 dmtykiasvr ihveiviqrl kifhilrnrv plsliphmkk vfsvcaalvn lqppiikq
//
LOCUS       XP_077523146             489 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144133986 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077523146
VERSION     XP_077523146.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667020.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..489
                     /product="uncharacterized protein LOC144133986 isoform X2"
                     /calculated_mol_wt=53997
     Region          14..>71
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          240..291
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          321..481
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..489
                     /gene="LOC144133986"
                     /coded_by="XM_077667020.1:126..1595"
                     /db_xref="GeneID:144133986"
ORIGIN      
        1 maeaalpkrg pkyccvvgcr nsaentkgld ppvklyrfpg kwyekerlqa winavrrvrw
       61 qrrlnqaiss rqlgagsenp srstddeatm hkpgtsitdc rhqdihmmdl sdaplemsaa
      121 vastistsdl hpsmsvcgtg eshfiakrda asqtkasvaq gmltillsat ngtdastqvv
      181 htemsdkvas tdgnwkkncg fqgfqslkek ekalqdlcgv tlqvfcllln llpppkyrrn
      241 tmsnedrlcl flaklrlgit ystlgvifsv apstastvfr ktlgilsial kdwvfmpsrr
      301 aiklslpapf kenypnctli idcteirtea psnpdcqhil yshykggytl kfligiipng
      361 misfvskvyg grhtdsfitq dsgflelvrp gdlilsdkgf psirttvegk gavllmppfn
      421 stggqmsddd mdmtykiasv rihveiviqr lkifhilrnr vplsliphmk kvfsvcaalv
      481 nlqppiikq
//
LOCUS       XP_077523151             233 aa            linear   INV 25-NOV-2025
DEFINITION  NPC intracellular cholesterol transporter 1-like [Amblyomma
            americanum].
ACCESSION   XP_077523151
VERSION     XP_077523151.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667025.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..233
                     /product="NPC intracellular cholesterol transporter
                     1-like"
                     /calculated_mol_wt=24838
     Region          21..>204
                     /region_name="NPC1_N"
                     /note="Niemann-Pick C1 N terminus; pfam16414"
                     /db_xref="CDD:465110"
     CDS             1..233
                     /gene="LOC144134000"
                     /coded_by="XM_077667025.1:190..891"
                     /db_xref="GeneID:144134000"
ORIGIN      
        1 mnaaarsall llcvfqraaa dcvfrghcad dedtdkaipc avhqppapla gvspwrlfad
       61 vcpqlaaeve gsrrvccdva qvqdlarele qparlgmakc pgcmlnfkdl lcrmtcspnq
      121 sqflavnsta kvgsgphvte mvyalrpdya qgvydsckdv rsvvlgiklm tlmcggrvlg
      181 cspqkwldfl gstpaeggys pfkihhvitd qpvaplgrpl tplgapilts cqp
//
LOCUS       XP_077523158             358 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-1 chain-like [Amblyomma americanum].
ACCESSION   XP_077523158
VERSION     XP_077523158.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667032.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..358
                     /product="tubulin beta-1 chain-like"
                     /calculated_mol_wt=40138
     Region          1..358
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..358
                     /gene="LOC144134010"
                     /coded_by="XM_077667032.1:160..1236"
                     /db_xref="GeneID:144134010"
ORIGIN      
        1 mdavrsrplg qlfrpdnfvf gqsgtgnnwa kghytegael vdsvldvvrk eaescdclqg
       61 fqlthslggg tgsgmgtlli skireeypdr imntysvvps pkvsdsvvep ynaalsvhql
      121 ventdetyci dnealydicf rtlklrtpty gdlnhlvsat msgvttclrf pgllngdlrk
      181 lavnmvpfpr lhffmtgfap ltsrgcqqyr altvpeltqq mfdaknmiaa cdprhgrclt
      241 vaavlrgrms mrevddqmln iqnkdssyfv ewipnnvkta vcdipprglk msatfianst
      301 aiqelfrris eqftamfhrk vflhwyteeg mdelefteae cnlndlvsey qqyqkata
//
LOCUS       XP_077523171             556 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134044 [Amblyomma americanum].
ACCESSION   XP_077523171
VERSION     XP_077523171.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667045.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..556
                     /product="uncharacterized protein LOC144134044"
                     /calculated_mol_wt=62101
     Region          <5..119
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            9
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238827"
     Region          312..408
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..556
                     /gene="LOC144134044"
                     /coded_by="XM_077667045.1:1..1671"
                     /db_xref="GeneID:144134044"
ORIGIN      
        1 mvtrgtpqga vlspllfnia mmrlpsqlar vegiqhalya dditiwtteg slgdmearlq
       61 qaasivdaya mdcgpqcapa ksellhvran pkdrtaihis lsggpireve elrilglfih
      121 hrlrpdstiv klkrvgeqvg rmirrvsnkr gglrgrdalr lvyafvtsrm iyavpylrtt
      181 kqdearidai irkatkrald lpvatsnakl kalgvlnsyq elreahlvnq ytrlmttapg
      241 rrllnrlhiq hdctpeeter ipvlwrhmlw vspvprnmdt dmhenrrqar awalerqhgs
      301 rpgvyyvdva gpsptgfyta avvhqemhvd rlsfrapnsm raeevaiala dahansriii
      361 tdsrkacdhy lageisplaa cilrrtatdt tpkriiwapc hqgvrgneaa daaaraliyr
      421 aphlsssgse tnqpllrfre iithysdnhr lfpapakgls ktekrtlrrl qtgtllctai
      481 lkhydpntdg rcphcgeicd ifhmvwsctq nphlppsptp sreawetall ncsslesqra
      541 lvkrardgal scdvpd
//
LOCUS       XP_077523176             556 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134052 [Amblyomma americanum].
ACCESSION   XP_077523176
VERSION     XP_077523176.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667050.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..556
                     /product="uncharacterized protein LOC144134052"
                     /calculated_mol_wt=62181
     Region          <5..115
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            9
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238827"
     CDS             1..556
                     /gene="LOC144134052"
                     /coded_by="XM_077667050.1:1..1671"
                     /db_xref="GeneID:144134052"
ORIGIN      
        1 mvtrgtpqga vlspllfnia mmrlpsqlar vegiqhalya dyitiwtteg slgdmearlq
       61 qaasivdaya mdcgpqcaaa ksellhvran pkdrtaihis lsggpireve elrilvlfih
      121 hrlrpdstiv klkrvgeqvg rmirrvsnrr gglrgrdalr lvyafvtsri lyavpylrtt
      181 kqdearidai irkatkrald lpvatsnakl kalgvlnsyq elreahlvnq ytrlmqtapg
      241 rrllnrlhiq hdctpeeter ipvlwrhmlw vsplprnmdt dmhenrrqar aralerqhgs
      301 rpgvyyvdva gpsptgfyta tvvhqemhvd rlsfrapnsm raeevaiala aahansriii
      361 tdsrkacdhy lageisplaa cilrrtatdt tpkriiwapc hqgvrgneaa daaaraliyr
      421 aphhsssgse tnqpllrfre iithysdnhr lfpapakgls ktekrtlrrl qtgtllctai
      481 lkhydpntdg rcphcgeicd ifhmvwacte nphlppsptp sreawetall ncsslesqra
      541 lvkrardgal scdvpd
//
LOCUS       XP_077523184             556 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134063 [Amblyomma americanum].
ACCESSION   XP_077523184
VERSION     XP_077523184.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667058.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..556
                     /product="uncharacterized protein LOC144134063"
                     /calculated_mol_wt=62181
     Region          <5..115
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            9
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238827"
     CDS             1..556
                     /gene="LOC144134063"
                     /coded_by="XM_077667058.1:1..1671"
                     /db_xref="GeneID:144134063"
ORIGIN      
        1 mvtrgtpqga vlspllfnia mmrlpsqlar vegiqhalya dyitiwtteg slgdmearlq
       61 qaasivdaya mdcgpqcaaa ksellhvran pkdrtaihis lsggpireve elrilvlfih
      121 hrlrpdstiv klkrvgeqvg rmirrvsnrr gglrgrdalr lvyafvtsri lyavpylrtt
      181 kqdearidai irkatkrald lpvatsnakl kalgvlnsyq elreahlvnq ytrlmqtapg
      241 rrllnrlhiq hdctpeeter ipvlwrhmlw vsplprnmdt dmhenrrqar aralerqhgs
      301 rpgvyyvdva gpsptgfyta tvvhqemhvd rlsfrapnsm raeevaiala aahansriii
      361 tdsrkacdhy lageisplaa cilrrtatdt tpkriiwapc hqgvrgneaa daaaraliyr
      421 aphhsssgse tnqpllrfre iithysdnhr lfpapakgls ktekrtlrrl qtgtllctai
      481 lkhydpntdg rcphcgeicd ifhmvwacte nphlppsptp sreawetall ncsslesqra
      541 lvkrardgal scdvpd
//
LOCUS       XP_077523204             437 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077523204
VERSION     XP_077523204.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667078.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..437
                     /product="tubulin beta-4 chain-like isoform X1"
                     /calculated_mol_wt=48975
     Region          1..437
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..437
                     /gene="LOC144134089"
                     /coded_by="XM_077667078.1:654..1967"
                     /db_xref="GeneID:144134089"
ORIGIN      
        1 mreivyiqtg qcgnqigakf wevisdehgi dpsgayygds dlqlerinvy yneapgdkyv
       61 prailvdlep gtmeavrsgp fgqlfrpdnf vfgqsgagnn wakghytega elvdsvldiv
      121 rkeaescdcm qgfqlthslg ggtgsgmgtl liskireeyp drimntysiv pspkvsdtvv
      181 epynatlslh qlventdetf cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffltgf apltsrgcqe yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqskdssy fvewipnnvk tavcdipprg
      361 lkmsatfian staiqelfkr iseqftamfr rkaflhwytg egmdelefte aesdlnelvs
      421 eyqqyqeata edegefe
//
LOCUS       XP_077523210             436 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077523210
VERSION     XP_077523210.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667084.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..436
                     /product="tubulin beta-4 chain-like isoform X2"
                     /calculated_mol_wt=48918
     Region          1..436
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..436
                     /gene="LOC144134089"
                     /coded_by="XM_077667084.1:654..1964"
                     /db_xref="GeneID:144134089"
ORIGIN      
        1 mreivyiqtg qcgnqigakf wevisdehgi dpsgayygds dlqlerinvy yneapdkyvp
       61 railvdlepg tmeavrsgpf gqlfrpdnfv fgqsgagnnw akghytegae lvdsvldivr
      121 keaescdcmq gfqlthslgg gtgsgmgtll iskireeypd rimntysivp spkvsdtvve
      181 pynatlslhq lventdetfc idnealydic frtlklttpt ygdlnhlvsa tmsgvttclr
      241 fpgqlnadlr klavnmvpfp rlhffltgfa pltsrgcqey raltvpeltq qmfdaknmma
      301 acdprhgryl tvaavfrgrm smrevddqml niqskdssyf vewipnnvkt avcdipprgl
      361 kmsatfians taiqelfkri seqftamfrr kaflhwytge gmdeleftea esdlnelvse
      421 yqqyqeatae degefe
//
LOCUS       XP_077523224             241 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134118 [Amblyomma americanum].
ACCESSION   XP_077523224
VERSION     XP_077523224.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667098.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..241
                     /product="uncharacterized protein LOC144134118"
                     /calculated_mol_wt=24498
     CDS             1..241
                     /gene="LOC144134118"
                     /coded_by="XM_077667098.1:10..735"
                     /db_xref="GeneID:144134118"
ORIGIN      
        1 mapgplqnhp ggvlgvsgqa stsmapgplq nhpggvlgvs gqastsmapg plqnhpggvl
       61 gvsgqasssm alapsqnlgi lsgvsrqast smapgplqnh pggvlgvsgq astsmapapl
      121 qdhpgalvev sgqvhlgngm yvsaerwrwl lsrgrdslfc kdtakllwgv snlrgksvtg
      181 aacrrflkag atqkpgltpe klaavgnafd ayiianptae dqkarrgkmn rfladmlrdi
      241 k
//
LOCUS       XP_077523244             437 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4B chain-like [Amblyomma americanum].
ACCESSION   XP_077523244
VERSION     XP_077523244.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667118.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..437
                     /product="tubulin beta-4B chain-like"
                     /calculated_mol_wt=49059
     Region          1..437
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..437
                     /gene="LOC144134147"
                     /coded_by="XM_077667118.1:51..1364"
                     /db_xref="GeneID:144134147"
ORIGIN      
        1 mreivyiqtg qcgnqigakf wevisdehgi dpsgtyygds dlqlerinvy yneapgdkyv
       61 prailvdlep gtmeavrsgp fgqlfrpdnf vfgqsgvgnn wakghytega elvdsvldvv
      121 rkeaescdcm qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlslh qlventdetf cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffltgf apltsrgcqe yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkessy fvewipnnvk tavcdipprg
      361 lkmsatfian staiqeifkr iseqftamfr rkaflhwytg egmdelefie aesdlnelvs
      421 eyqqyqeata edegefe
//
LOCUS       XP_077523250             437 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077523250
VERSION     XP_077523250.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667124.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..437
                     /product="tubulin beta chain-like isoform X1"
                     /calculated_mol_wt=48902
     Region          1..437
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..437
                     /gene="LOC144134157"
                     /coded_by="XM_077667124.1:66..1379"
                     /db_xref="GeneID:144134157"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayygds dlqlerinvy yneapgdkyv
       61 prailvdlep gtmeavrsgp fgqlfrpdnf vfgqggagnn wakghytega elvdsvldvv
      121 rkeaescdcm qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdetf cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffltgf apltsrgcqe yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkdssy fvewipnnvk tavcdipprg
      361 lkmsatfian staiqelfkr iseqftamfr rkaflhwytg egmdelefie aesdlnelvs
      421 eyqqyqeata ddegefe
//
LOCUS       XP_077523254             390 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077523254
VERSION     XP_077523254.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667128.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..390
                     /product="tubulin beta chain-like isoform X2"
                     /calculated_mol_wt=43722
     Region          9..390
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..390
                     /gene="LOC144134157"
                     /coded_by="XM_077667128.1:85..1257"
                     /db_xref="GeneID:144134157"
ORIGIN      
        1 mrkpdwsegd kyvprailvd lepgtmeavr sgpfgqlfrp dnfvfgqgga gnnwakghyt
       61 egaelvdsvl dvvrkeaesc dcmqgfqlth slgggtgsgm gtlliskire eypdrimnty
      121 svvpspkvsd tvvepynatl svhqlventd etfcidneal ydicfrtlkl ttptygdlnh
      181 lvsatmsgvt tclrfpgqln adlrklavnm vpfprlhffl tgfapltsrg cqeyraltvp
      241 eltqqmfdak nmmaacdprh gryltvaavf rgrmsmrevd dqmlniqnkd ssyfvewipn
      301 nvktavcdip prglkmsatf ianstaiqel fkriseqfta mfrrkaflhw ytgegmdele
      361 fieaesdlne lvseyqqyqe ataddegefe
//
LOCUS       XP_077523261             389 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4B chain-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077523261
VERSION     XP_077523261.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667135.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..389
                     /product="tubulin beta-4B chain-like isoform X3"
                     /calculated_mol_wt=43665
     Region          7..389
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..389
                     /gene="LOC144134157"
                     /coded_by="XM_077667135.1:85..1254"
                     /db_xref="GeneID:144134157"
ORIGIN      
        1 mrkpdwsedk yvprailvdl epgtmeavrs gpfgqlfrpd nfvfgqggag nnwakghyte
       61 gaelvdsvld vvrkeaescd cmqgfqlths lgggtgsgmg tlliskiree ypdrimntys
      121 vvpspkvsdt vvepynatls vhqlventde tfcidnealy dicfrtlklt tptygdlnhl
      181 vsatmsgvtt clrfpgqlna dlrklavnmv pfprlhfflt gfapltsrgc qeyraltvpe
      241 ltqqmfdakn mmaacdprhg ryltvaavfr grmsmrevdd qmlniqnkds syfvewipnn
      301 vktavcdipp rglkmsatfi anstaiqelf kriseqftam frrkaflhwy tgegmdelef
      361 ieaesdlnel vseyqqyqea taddegefe
//
LOCUS       XP_077523268             437 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077523268
VERSION     XP_077523268.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667142.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..437
                     /product="tubulin beta-4 chain-like isoform X1"
                     /calculated_mol_wt=48976
     Region          1..437
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..437
                     /gene="LOC144134181"
                     /coded_by="XM_077667142.1:162..1475"
                     /db_xref="GeneID:144134181"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayygds dlqlerinvy yneapgdkyv
       61 prailvdlep gtmeavrsgr fgqlfrpdnf vfgqggagnn wakghytega elvdsvldvv
      121 rkeaescdcm qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdetf cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffltgf apltsrgcqe yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkessy fvewipnnvk tavcdipprg
      361 lkmsatfian staiqelfkr iseqftamfr rkaflhwytg egmdelefte aesnlnelvs
      421 eyqqyqeata edegefe
//
LOCUS       XP_077523275             389 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4B chain-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077523275
VERSION     XP_077523275.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667149.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..389
                     /product="tubulin beta-4B chain-like isoform X2"
                     /calculated_mol_wt=43739
     Region          7..389
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..389
                     /gene="LOC144134181"
                     /coded_by="XM_077667149.1:194..1363"
                     /db_xref="GeneID:144134181"
ORIGIN      
        1 mrkpdwsedk yvprailvdl epgtmeavrs grfgqlfrpd nfvfgqggag nnwakghyte
       61 gaelvdsvld vvrkeaescd cmqgfqlths lgggtgsgmg tlliskiree ypdrimntys
      121 vvpspkvsdt vvepynatls vhqlventde tfcidnealy dicfrtlklt tptygdlnhl
      181 vsatmsgvtt clrfpgqlna dlrklavnmv pfprlhfflt gfapltsrgc qeyraltvpe
      241 ltqqmfdakn mmaacdprhg ryltvaavfr grmsmrevdd qmlniqnkes syfvewipnn
      301 vktavcdipp rglkmsatfi anstaiqelf kriseqftam frrkaflhwy tgegmdelef
      361 teaesnlnel vseyqqyqea taedegefe
//
LOCUS       XP_077523285             437 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077523285
VERSION     XP_077523285.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667159.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..437
                     /product="tubulin beta chain-like isoform X1"
                     /calculated_mol_wt=48902
     Region          1..437
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..437
                     /gene="LOC144134198"
                     /coded_by="XM_077667159.1:188..1501"
                     /db_xref="GeneID:144134198"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayygds dlqlerinvy yneapgdkyv
       61 prailvdlep gtmeavrsgp fgqlfrpdnf vfgqggagnn wakghytega elvdsvldvv
      121 rkeaescdcm qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdetf cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffltgf apltsrgcqe yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkdssy fvewipnnvk tavcdipprg
      361 lkmsatfian staiqelfkr iseqftamfr rkaflhwytg egmdelefie aesdlnelvs
      421 eyqqyqeata ddegefe
//
LOCUS       XP_077523292             436 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077523292
VERSION     XP_077523292.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667166.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..436
                     /product="tubulin beta chain-like isoform X2"
                     /calculated_mol_wt=48845
     Region          1..436
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..436
                     /gene="LOC144134198"
                     /coded_by="XM_077667166.1:188..1498"
                     /db_xref="GeneID:144134198"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayygds dlqlerinvy yneapdkyvp
       61 railvdlepg tmeavrsgpf gqlfrpdnfv fgqggagnnw akghytegae lvdsvldvvr
      121 keaescdcmq gfqlthslgg gtgsgmgtll iskireeypd rimntysvvp spkvsdtvve
      181 pynatlsvhq lventdetfc idnealydic frtlklttpt ygdlnhlvsa tmsgvttclr
      241 fpgqlnadlr klavnmvpfp rlhffltgfa pltsrgcqey raltvpeltq qmfdaknmma
      301 acdprhgryl tvaavfrgrm smrevddqml niqnkdssyf vewipnnvkt avcdipprgl
      361 kmsatfians taiqelfkri seqftamfrr kaflhwytge gmdelefiea esdlnelvse
      421 yqqyqeatad degefe
//
LOCUS       XP_077523298             390 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077523298
VERSION     XP_077523298.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667172.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..390
                     /product="tubulin beta chain-like isoform X3"
                     /calculated_mol_wt=43722
     Region          9..390
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..390
                     /gene="LOC144134198"
                     /coded_by="XM_077667172.1:220..1392"
                     /db_xref="GeneID:144134198"
ORIGIN      
        1 mrkpdwsegd kyvprailvd lepgtmeavr sgpfgqlfrp dnfvfgqgga gnnwakghyt
       61 egaelvdsvl dvvrkeaesc dcmqgfqlth slgggtgsgm gtlliskire eypdrimnty
      121 svvpspkvsd tvvepynatl svhqlventd etfcidneal ydicfrtlkl ttptygdlnh
      181 lvsatmsgvt tclrfpgqln adlrklavnm vpfprlhffl tgfapltsrg cqeyraltvp
      241 eltqqmfdak nmmaacdprh gryltvaavf rgrmsmrevd dqmlniqnkd ssyfvewipn
      301 nvktavcdip prglkmsatf ianstaiqel fkriseqfta mfrrkaflhw ytgegmdele
      361 fieaesdlne lvseyqqyqe ataddegefe
//
LOCUS       XP_077523305             389 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4B chain-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077523305
VERSION     XP_077523305.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667179.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..389
                     /product="tubulin beta-4B chain-like isoform X4"
                     /calculated_mol_wt=43665
     Region          7..389
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..389
                     /gene="LOC144134198"
                     /coded_by="XM_077667179.1:220..1389"
                     /db_xref="GeneID:144134198"
ORIGIN      
        1 mrkpdwsedk yvprailvdl epgtmeavrs gpfgqlfrpd nfvfgqggag nnwakghyte
       61 gaelvdsvld vvrkeaescd cmqgfqlths lgggtgsgmg tlliskiree ypdrimntys
      121 vvpspkvsdt vvepynatls vhqlventde tfcidnealy dicfrtlklt tptygdlnhl
      181 vsatmsgvtt clrfpgqlna dlrklavnmv pfprlhfflt gfapltsrgc qeyraltvpe
      241 ltqqmfdakn mmaacdprhg ryltvaavfr grmsmrevdd qmlniqnkds syfvewipnn
      301 vktavcdipp rglkmsatfi anstaiqelf kriseqftam frrkaflhwy tgegmdelef
      361 ieaesdlnel vseyqqyqea taddegefe
//
LOCUS       XP_077523310             437 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077523310
VERSION     XP_077523310.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667184.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..437
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=48918
     Region          1..437
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..437
                     /gene="LOC144134224"
                     /coded_by="XM_077667184.1:70..1383"
                     /db_xref="GeneID:144134224"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayygds dlqlerinvy yneapgdkyv
       61 prailvdlep gtmeavrsgp fgqlfrpdnf vfgqggagnn wakghytega elvdsvldvv
      121 rkeaescdcm qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdetf cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffltgf apltsrgcqe yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkessy fvewipnnvk tavcdipprg
      361 lkmsatfian staiqelfkr iseqftamfr rkaflhwytg egmdelefte aesdlnelvs
      421 eyqqyqeata edegefe
//
LOCUS       XP_077523320             437 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077523320
VERSION     XP_077523320.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667194.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..437
                     /product="tubulin beta chain-like isoform X1"
                     /calculated_mol_wt=48919
     Region          1..437
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..437
                     /gene="LOC144134233"
                     /coded_by="XM_077667194.1:52..1365"
                     /db_xref="GeneID:144134233"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayygds dlqlerinvy yneapgdkyv
       61 prailvdlep gtmeavrsgp fgqlfrpdnf vfgqggagnn wakghytega elvdsvldvv
      121 rmeaescdcm qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlslh qlventdetf cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffltgf apltsrgcqe yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkdssy fvewipnnvk tavcdipprg
      361 lkmsatfian staiqelfkr iseqftamfr rkaflhwytg egmdelefie aesdlnelvs
      421 eyqqyqeata ddegefe
//
LOCUS       XP_077523325             390 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077523325
VERSION     XP_077523325.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667199.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..390
                     /product="tubulin beta chain-like isoform X2"
                     /calculated_mol_wt=43739
     Region          9..390
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..390
                     /gene="LOC144134233"
                     /coded_by="XM_077667199.1:83..1255"
                     /db_xref="GeneID:144134233"
ORIGIN      
        1 mrkpdwsegd kyvprailvd lepgtmeavr sgpfgqlfrp dnfvfgqgga gnnwakghyt
       61 egaelvdsvl dvvrmeaesc dcmqgfqlth slgggtgsgm gtlliskire eypdrimnty
      121 svvpspkvsd tvvepynatl slhqlventd etfcidneal ydicfrtlkl ttptygdlnh
      181 lvsatmsgvt tclrfpgqln adlrklavnm vpfprlhffl tgfapltsrg cqeyraltvp
      241 eltqqmfdak nmmaacdprh gryltvaavf rgrmsmrevd dqmlniqnkd ssyfvewipn
      301 nvktavcdip prglkmsatf ianstaiqel fkriseqfta mfrrkaflhw ytgegmdele
      361 fieaesdlne lvseyqqyqe ataddegefe
//
LOCUS       XP_077523330             389 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4B chain-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077523330
VERSION     XP_077523330.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667204.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..389
                     /product="tubulin beta-4B chain-like isoform X3"
                     /calculated_mol_wt=43682
     Region          7..389
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..389
                     /gene="LOC144134233"
                     /coded_by="XM_077667204.1:83..1252"
                     /db_xref="GeneID:144134233"
ORIGIN      
        1 mrkpdwsedk yvprailvdl epgtmeavrs gpfgqlfrpd nfvfgqggag nnwakghyte
       61 gaelvdsvld vvrmeaescd cmqgfqlths lgggtgsgmg tlliskiree ypdrimntys
      121 vvpspkvsdt vvepynatls lhqlventde tfcidnealy dicfrtlklt tptygdlnhl
      181 vsatmsgvtt clrfpgqlna dlrklavnmv pfprlhfflt gfapltsrgc qeyraltvpe
      241 ltqqmfdakn mmaacdprhg ryltvaavfr grmsmrevdd qmlniqnkds syfvewipnn
      301 vktavcdipp rglkmsatfi anstaiqelf kriseqftam frrkaflhwy tgegmdelef
      361 ieaesdlnel vseyqqyqea taddegefe
//
LOCUS       XP_077523338             437 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077523338
VERSION     XP_077523338.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667212.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..437
                     /product="tubulin beta chain-like isoform X1"
                     /calculated_mol_wt=48902
     Region          1..437
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..437
                     /gene="LOC144134253"
                     /coded_by="XM_077667212.1:440..1753"
                     /db_xref="GeneID:144134253"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayygds dlqlerinvy yneapgdkyv
       61 prailvdlep gtmeavrsgp fgqlfrpdnf vfgqggagnn wakghytega elvdsvldvv
      121 rkeaescdcm qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdetf cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffltgf apltsrgcqe yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkdssy fvewipnnvk tavcdipprg
      361 lkmsatfian staiqelfkr iseqftamfr rkaflhwytg egmdelefie aesdlnelvs
      421 eyqqyqeata ddegefe
//
LOCUS       XP_077523340             436 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077523340
VERSION     XP_077523340.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667214.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..436
                     /product="tubulin beta chain-like isoform X2"
                     /calculated_mol_wt=48845
     Region          1..436
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..436
                     /gene="LOC144134253"
                     /coded_by="XM_077667214.1:440..1750"
                     /db_xref="GeneID:144134253"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayygds dlqlerinvy yneapdkyvp
       61 railvdlepg tmeavrsgpf gqlfrpdnfv fgqggagnnw akghytegae lvdsvldvvr
      121 keaescdcmq gfqlthslgg gtgsgmgtll iskireeypd rimntysvvp spkvsdtvve
      181 pynatlsvhq lventdetfc idnealydic frtlklttpt ygdlnhlvsa tmsgvttclr
      241 fpgqlnadlr klavnmvpfp rlhffltgfa pltsrgcqey raltvpeltq qmfdaknmma
      301 acdprhgryl tvaavfrgrm smrevddqml niqnkdssyf vewipnnvkt avcdipprgl
      361 kmsatfians taiqelfkri seqftamfrr kaflhwytge gmdelefiea esdlnelvse
      421 yqqyqeatad degefe
//
LOCUS       XP_077523344             390 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077523344
VERSION     XP_077523344.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667218.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..390
                     /product="tubulin beta chain-like isoform X3"
                     /calculated_mol_wt=43722
     Region          9..390
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..390
                     /gene="LOC144134253"
                     /coded_by="XM_077667218.1:471..1643"
                     /db_xref="GeneID:144134253"
ORIGIN      
        1 mrkpdwsegd kyvprailvd lepgtmeavr sgpfgqlfrp dnfvfgqgga gnnwakghyt
       61 egaelvdsvl dvvrkeaesc dcmqgfqlth slgggtgsgm gtlliskire eypdrimnty
      121 svvpspkvsd tvvepynatl svhqlventd etfcidneal ydicfrtlkl ttptygdlnh
      181 lvsatmsgvt tclrfpgqln adlrklavnm vpfprlhffl tgfapltsrg cqeyraltvp
      241 eltqqmfdak nmmaacdprh gryltvaavf rgrmsmrevd dqmlniqnkd ssyfvewipn
      301 nvktavcdip prglkmsatf ianstaiqel fkriseqfta mfrrkaflhw ytgegmdele
      361 fieaesdlne lvseyqqyqe ataddegefe
//
LOCUS       XP_077523346             389 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4B chain-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077523346
VERSION     XP_077523346.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667220.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..389
                     /product="tubulin beta-4B chain-like isoform X4"
                     /calculated_mol_wt=43665
     Region          7..389
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..389
                     /gene="LOC144134253"
                     /coded_by="XM_077667220.1:471..1640"
                     /db_xref="GeneID:144134253"
ORIGIN      
        1 mrkpdwsedk yvprailvdl epgtmeavrs gpfgqlfrpd nfvfgqggag nnwakghyte
       61 gaelvdsvld vvrkeaescd cmqgfqlths lgggtgsgmg tlliskiree ypdrimntys
      121 vvpspkvsdt vvepynatls vhqlventde tfcidnealy dicfrtlklt tptygdlnhl
      181 vsatmsgvtt clrfpgqlna dlrklavnmv pfprlhfflt gfapltsrgc qeyraltvpe
      241 ltqqmfdakn mmaacdprhg ryltvaavfr grmsmrevdd qmlniqnkds syfvewipnn
      301 vktavcdipp rglkmsatfi anstaiqelf kriseqftam frrkaflhwy tgegmdelef
      361 ieaesdlnel vseyqqyqea taddegefe
//
LOCUS       XP_077523354             437 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077523354
VERSION     XP_077523354.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667228.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..437
                     /product="tubulin beta chain-like isoform X1"
                     /calculated_mol_wt=48902
     Region          1..437
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..437
                     /gene="LOC144134276"
                     /coded_by="XM_077667228.1:444..1757"
                     /db_xref="GeneID:144134276"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayygds dlqlerinvy yneapgdkyv
       61 prailvdlep gtmeavrsgp fgqlfrpdnf vfgqggagnn wakghytega elvdsvldvv
      121 rkeaescdcm qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdetf cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffltgf apltsrgcqe yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkdssy fvewipnnvk tavcdipprg
      361 lkmsatfian staiqelfkr iseqftamfr rkaflhwytg egmdelefie aesdlnelvs
      421 eyqqyqeata ddegefe
//
LOCUS       XP_077523360             436 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077523360
VERSION     XP_077523360.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667234.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..436
                     /product="tubulin beta chain-like isoform X2"
                     /calculated_mol_wt=48845
     Region          1..436
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..436
                     /gene="LOC144134276"
                     /coded_by="XM_077667234.1:444..1754"
                     /db_xref="GeneID:144134276"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayygds dlqlerinvy yneapdkyvp
       61 railvdlepg tmeavrsgpf gqlfrpdnfv fgqggagnnw akghytegae lvdsvldvvr
      121 keaescdcmq gfqlthslgg gtgsgmgtll iskireeypd rimntysvvp spkvsdtvve
      181 pynatlsvhq lventdetfc idnealydic frtlklttpt ygdlnhlvsa tmsgvttclr
      241 fpgqlnadlr klavnmvpfp rlhffltgfa pltsrgcqey raltvpeltq qmfdaknmma
      301 acdprhgryl tvaavfrgrm smrevddqml niqnkdssyf vewipnnvkt avcdipprgl
      361 kmsatfians taiqelfkri seqftamfrr kaflhwytge gmdelefiea esdlnelvse
      421 yqqyqeatad degefe
//
LOCUS       XP_077523363             390 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077523363
VERSION     XP_077523363.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667237.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..390
                     /product="tubulin beta chain-like isoform X3"
                     /calculated_mol_wt=43722
     Region          9..390
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..390
                     /gene="LOC144134276"
                     /coded_by="XM_077667237.1:475..1647"
                     /db_xref="GeneID:144134276"
ORIGIN      
        1 mrkpdwsegd kyvprailvd lepgtmeavr sgpfgqlfrp dnfvfgqgga gnnwakghyt
       61 egaelvdsvl dvvrkeaesc dcmqgfqlth slgggtgsgm gtlliskire eypdrimnty
      121 svvpspkvsd tvvepynatl svhqlventd etfcidneal ydicfrtlkl ttptygdlnh
      181 lvsatmsgvt tclrfpgqln adlrklavnm vpfprlhffl tgfapltsrg cqeyraltvp
      241 eltqqmfdak nmmaacdprh gryltvaavf rgrmsmrevd dqmlniqnkd ssyfvewipn
      301 nvktavcdip prglkmsatf ianstaiqel fkriseqfta mfrrkaflhw ytgegmdele
      361 fieaesdlne lvseyqqyqe ataddegefe
//
LOCUS       XP_077523367             389 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4B chain-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077523367
VERSION     XP_077523367.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667241.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..389
                     /product="tubulin beta-4B chain-like isoform X4"
                     /calculated_mol_wt=43665
     Region          7..389
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..389
                     /gene="LOC144134276"
                     /coded_by="XM_077667241.1:475..1644"
                     /db_xref="GeneID:144134276"
ORIGIN      
        1 mrkpdwsedk yvprailvdl epgtmeavrs gpfgqlfrpd nfvfgqggag nnwakghyte
       61 gaelvdsvld vvrkeaescd cmqgfqlths lgggtgsgmg tlliskiree ypdrimntys
      121 vvpspkvsdt vvepynatls vhqlventde tfcidnealy dicfrtlklt tptygdlnhl
      181 vsatmsgvtt clrfpgqlna dlrklavnmv pfprlhfflt gfapltsrgc qeyraltvpe
      241 ltqqmfdakn mmaacdprhg ryltvaavfr grmsmrevdd qmlniqnkds syfvewipnn
      301 vktavcdipp rglkmsatfi anstaiqelf kriseqftam frrkaflhwy tgegmdelef
      361 ieaesdlnel vseyqqyqea taddegefe
//
LOCUS       XP_077523373             436 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like [Amblyomma americanum].
ACCESSION   XP_077523373
VERSION     XP_077523373.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667247.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..436
                     /product="tubulin beta chain-like"
                     /calculated_mol_wt=48879
     Region          1..436
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..436
                     /gene="LOC144134302"
                     /coded_by="XM_077667247.1:97..1407"
                     /db_xref="GeneID:144134302"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayygds dlqlermnvy yneapdkyvp
       61 railvdlepg tmeavrsgpf gqlfrpdnfv fgqggagnnw akghytegae lvdsvldvvr
      121 keaescdcmq gfqlthslgg gtgsgmgtll iskireeypd rimntysvvp spkvsdtvve
      181 pynatlslhq lventdetfc idnealydic frtlklttpt ygdlnhlvsa tmsgvttclr
      241 fpgqlnadlr klavnmvpfp rlhffltgfa pltsrgcqey raltvpeltq qmfdaknmma
      301 acdprhgryl tvaavfrgrm smrevddqml niqnkessyf vewipnnvkt avcdipprgl
      361 kmsatfians taiqelfkri seqftamfrr kaflhwytge gmdeleftea esdlnelvse
      421 yqqyqeatad degefe
//
LOCUS       XP_077523410             211 aa            linear   INV 25-NOV-2025
DEFINITION  sodium-coupled neutral amino acid transporter 7-like [Amblyomma
            americanum].
ACCESSION   XP_077523410
VERSION     XP_077523410.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667284.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..211
                     /product="sodium-coupled neutral amino acid transporter
                     7-like"
                     /calculated_mol_wt=23071
     Region          <1..191
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     CDS             1..211
                     /gene="LOC144134354"
                     /coded_by="XM_077667284.1:7..642"
                     /db_xref="GeneID:144134354"
ORIGIN      
        1 mlvpiyanmr drrlanlaka tvmttfflfv iyslmgtfgy maygsavkpd imqmfdasnp
       61 wvlfgigali vkmvttypll afcgrgafdg lyaeltklpt kefiegeprr riyittgwfl
      121 ttvalatfts nvgvviellg claaanifif pglcllgvfh qqekfglkrp mvvfaigglm
      181 vvigsfvfgv vlldtilndi tkggthhllc t
//
LOCUS       XP_077523419             465 aa            linear   INV 25-NOV-2025
DEFINITION  sodium-coupled neutral amino acid transporter 7-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077523419
VERSION     XP_077523419.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667293.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..465
                     /product="sodium-coupled neutral amino acid transporter
                     7-like isoform X1"
                     /calculated_mol_wt=49985
     Region          50..412
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     CDS             1..465
                     /gene="LOC144134366"
                     /coded_by="XM_077667293.1:165..1562"
                     /db_xref="GeneID:144134366"
ORIGIN      
        1 magtasergm paevsrvsgt srsaskmtnp tppkdvaqaa pppentegvg tmtaysvavv
       61 matsalgagl lhfpaaynva ggilmatvvq lvlvvalggt iltlafcadv nndgtyhdil
      121 lsmcgvkwry aaavsvlltc ygvcvtyliv igdqydrifa slhgpdfcht wylareftit
      181 atavvivlpl nllqhieflh yastlgiaav lypcvltcvv yfteprpkds yvrvwpeslf
      241 dvllstpvfc fayqshevvi payaamrers lwnmakaiay cmvilmvays mvgcfgylsy
      301 gstvrpdvme mfdatkawvl vglvalifkm avtyplpalc arnaieelyl evrggpaddr
      361 araharkakl slawfastvg mavlpldigv aikflgclaa lnifvfpgmc ligytkhkgv
      421 ptahlhwhll lgslmvlaga vlftavllhv vlvelvsghv vqlcs
//
LOCUS       XP_077523425             465 aa            linear   INV 25-NOV-2025
DEFINITION  sodium-coupled neutral amino acid transporter 7-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077523425
VERSION     XP_077523425.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667299.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..465
                     /product="sodium-coupled neutral amino acid transporter
                     7-like isoform X2"
                     /calculated_mol_wt=49985
     Region          50..412
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     CDS             1..465
                     /gene="LOC144134366"
                     /coded_by="XM_077667299.1:59..1456"
                     /db_xref="GeneID:144134366"
ORIGIN      
        1 magtasergm paevsrvsgt srsaskmtnp tppkdvaqaa pppentegvg tmtaysvavv
       61 matsalgagl lhfpaaynva ggilmatvvq lvlvvalggt iltlafcadv nndgtyhdil
      121 lsmcgvkwry aaavsvlltc ygvcvtyliv igdqydrifa slhgpdfcht wylareftit
      181 atavvivlpl nllqhieflh yastlgiaav lypcvltcvv yfteprpkds yvrvwpeslf
      241 dvllstpvfc fayqshevvi payaamrers lwnmakaiay cmvilmvays mvgcfgylsy
      301 gstvrpdvme mfdatkawvl vglvalifkm avtyplpalc arnaieelyl evrggpaddr
      361 araharkakl slawfastvg mavlpldigv aikflgclaa lnifvfpgmc ligytkhkgv
      421 ptahlhwhll lgslmvlaga vlftavllhv vlvelvsghv vqlcs
//
LOCUS       XP_077523435             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077523435
VERSION     XP_077523435.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667309.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=49588
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144134384"
                     /coded_by="XM_077667309.1:286..1617"
                     /db_xref="GeneID:144134384"
ORIGIN      
        1 mreivyiqtg qcgnqigakf weiisdehgi dpsgayygds dlqlerinvy yneapggkyv
       61 prtilvdlep gtmdavrsgp fgqlfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaescdcm qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdetf cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffltgf apltsrgcqe yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkdssy fvewipnnvk tavcdipprg
      361 lkmsatfian staiqelfkr iseqftamfr rkaflhwytg egmdelefte aesdlnelvs
      421 eyqqyqeatp edegefeetq aea
//
LOCUS       XP_077523451             102 aa            linear   INV 25-NOV-2025
DEFINITION  obstructor-I [Amblyomma americanum].
ACCESSION   XP_077523451
VERSION     XP_077523451.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667325.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..102
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..102
                     /product="obstructor-I"
                     /calculated_mol_wt=10720
     Region          34..82
                     /region_name="CBM_14"
                     /note="Chitin binding Peritrophin-A domain; pfam01607"
                     /db_xref="CDD:426342"
     CDS             1..102
                     /gene="obst-I"
                     /coded_by="XM_077667325.1:14..322"
                     /db_xref="GeneID:144134402"
ORIGIN      
        1 mlmalalwmt asasasdpwv paelsgcggp cwspsglfpd padccgfysc ascrayrlrc
       61 apgtvfdrrl glcnhpqdap aclapnpgcy lyylsssftg kk
//
LOCUS       XP_077523462             201 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134412 [Amblyomma americanum].
ACCESSION   XP_077523462
VERSION     XP_077523462.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667336.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..201
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..201
                     /product="uncharacterized protein LOC144134412"
                     /calculated_mol_wt=21557
     CDS             1..201
                     /gene="LOC144134412"
                     /coded_by="XM_077667336.1:12..617"
                     /db_xref="GeneID:144134412"
ORIGIN      
        1 mseagsegkl dspdlepned didvespver esleeyspda gspeaalneg eivltaegie
       61 agdgvepavd veagvgiegd egievpaaar drkfyramds imvakhdkpr rrvpentdss
      121 sslmafcvag glalvimaic tvsfvivkmm igveqsydft faeasmdprd yadddtgqnt
      181 sqatpemrpe nttfpsmfsw f
//
LOCUS       XP_077523490             111 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134448 [Amblyomma americanum].
ACCESSION   XP_077523490
VERSION     XP_077523490.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667364.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..111
                     /product="uncharacterized protein LOC144134448"
                     /calculated_mol_wt=11723
     CDS             1..111
                     /gene="LOC144134448"
                     /coded_by="XM_077667364.1:164..499"
                     /db_xref="GeneID:144134448"
ORIGIN      
        1 mtpkplpiph prpevrrhgd gdvgklrirt awatmfsacg npvtsgglpi sqaeppvpgc
       61 grhrqdagtr qrparkahpa vvagvfqnly fpyegvlaap ssaviinvss r
//
LOCUS       XP_077523509             136 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134471 [Amblyomma americanum].
ACCESSION   XP_077523509
VERSION     XP_077523509.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667383.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..136
                     /product="uncharacterized protein LOC144134471"
                     /calculated_mol_wt=14647
     CDS             1..136
                     /gene="LOC144134471"
                     /coded_by="XM_077667383.1:81..491"
                     /db_xref="GeneID:144134471"
ORIGIN      
        1 mlypldarvc llsrcwerpl wqfgdmtpep lstpnprpai rrhgvgdvgk qrirtawatm
       61 fsacgnsvts gappiaqaep pvpgcgrhcq dggtrqrpar kahpavvagv fqnlyfpyeg
      121 vlaapssari invssr
//
LOCUS       XP_077523519             110 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134481 [Amblyomma americanum].
ACCESSION   XP_077523519
VERSION     XP_077523519.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667393.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..110
                     /product="uncharacterized protein LOC144134481"
                     /calculated_mol_wt=11453
     CDS             1..110
                     /gene="LOC144134481"
                     /coded_by="XM_077667393.1:200..532"
                     /db_xref="GeneID:144134481"
ORIGIN      
        1 mtpeplstpd prpairchgd gdvgklrlrt awatmfsacg ntvtsgglpi sqaeppvpgc
       61 grrcqdagtr qrparkahpa vvagvfqnly fpyegvlaap ssaliivssr
//
LOCUS       XP_077523530             111 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134492 [Amblyomma americanum].
ACCESSION   XP_077523530
VERSION     XP_077523530.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667404.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..111
                     /product="uncharacterized protein LOC144134492"
                     /calculated_mol_wt=11639
     CDS             1..111
                     /gene="LOC144134492"
                     /coded_by="XM_077667404.1:259..594"
                     /db_xref="GeneID:144134492"
ORIGIN      
        1 mtpeplstpd prfvirrhgv gdvgklrirt awatmfsacg ntvtsgappi aqaeppapgc
       61 grhrqdggtr qrparkahpa vvagvfqnlc fpyegvlaap ssariinvss r
//
LOCUS       XP_077523540             135 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134503 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077523540
VERSION     XP_077523540.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667414.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..135
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..135
                     /product="uncharacterized protein LOC144134503 isoform X1"
                     /calculated_mol_wt=14650
     CDS             1..135
                     /gene="LOC144134503"
                     /coded_by="XM_077667414.1:2139..2546"
                     /db_xref="GeneID:144134503"
ORIGIN      
        1 meetvicvcl psrcwehplw qlgdmtpepl stpdprpair rhgdgdvgkl rirtawatmf
       61 sacgntvtsr glpisqaers vpgcgrhrqd ggtrqrlark ahpavvagvf qnmyfpyegv
      121 laapssalii nvssr
//
LOCUS       XP_077523547             111 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134503 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077523547
VERSION     XP_077523547.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667421.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..111
                     /product="uncharacterized protein LOC144134503 isoform X2"
                     /calculated_mol_wt=11823
     CDS             1..111
                     /gene="LOC144134503"
                     /coded_by="XM_077667421.1:191..526"
                     /db_xref="GeneID:144134503"
ORIGIN      
        1 mtpeplstpd prpairrhgd gdvgklrirt awatmfsacg ntvtsrglpi sqaersvpgc
       61 grhrqdggtr qrlarkahpa vvagvfqnmy fpyegvlaap ssaliinvss r
//
LOCUS       XP_077523563             114 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134526 [Amblyomma americanum].
ACCESSION   XP_077523563
VERSION     XP_077523563.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667437.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..114
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..114
                     /product="uncharacterized protein LOC144134526"
                     /calculated_mol_wt=12672
     CDS             1..114
                     /gene="LOC144134526"
                     /coded_by="XM_077667437.1:134..478"
                     /db_xref="GeneID:144134526"
ORIGIN      
        1 mflhrtqlaa lhynenadrl qgrtslgeqr wavkypkgrk skpvacpfke patndyignl
       61 leiveellds ssfgaahlca lpppppplts yfdpvdkaal vrdhlkfctt krqp
//
LOCUS       XP_077523603             148 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134585 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077523603
VERSION     XP_077523603.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667477.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="uncharacterized protein LOC144134585 isoform X1"
                     /calculated_mol_wt=16759
     CDS             1..148
                     /gene="LOC144134585"
                     /coded_by="XM_077667477.1:336..782"
                     /db_xref="GeneID:144134585"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedrrr rhlnrrltat mkrlsgvhvl
       61 nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar pgevyvvhrc
      121 rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077523609              98 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134585 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077523609
VERSION     XP_077523609.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667483.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..98
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..98
                     /product="uncharacterized protein LOC144134585 isoform X2"
                     /calculated_mol_wt=10604
     CDS             1..98
                     /gene="LOC144134585"
                     /coded_by="XM_077667483.1:415..711"
                     /db_xref="GeneID:144134585"
ORIGIN      
        1 mkrlsgvhvl nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar
       61 pgevyvvhrc rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077523630             541 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134629 [Amblyomma americanum].
ACCESSION   XP_077523630
VERSION     XP_077523630.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667504.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..541
                     /product="uncharacterized protein LOC144134629"
                     /calculated_mol_wt=60246
     Region          <9..99
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Region          284..393
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     Site            order(288..291,319..320,323,342,379)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260008"
     CDS             1..541
                     /gene="LOC144134629"
                     /coded_by="XM_077667504.1:1..1626"
                     /db_xref="GeneID:144134629"
ORIGIN      
        1 mknlpvhlek vpgvqhalya ddisiwatqg slgemeaslp eaatavneya krcglhcspq
       61 ksefvhirpn kkctnkielr lgsepiperk eiralglfih qrrlatttld klkkvgdqvg
      121 rmvrrvsnkk gglrskdalr manafvtsri lystpylrln khdqniievi irkrakkald
      181 lpittsnlrl qglgmtnsfd elreahltgq ytrlsqtpsg rrllarlhie httlteervr
      241 lsqewryalh vrplpinmsr ddhegrrrar aealahhygr kpgvyyvdas gphqggwyta
      301 avihesntvn gltfkarsvt qaeevaiala athsdsktii tdsrgacrnv qqgwvpylay
      361 rllqnssyvg gpshrfivwa pahsglggne aadaaarals nratsspass eevaeatpty
      421 tykeitqlyk drhklyphpc kgltraeerl flrlvtntmm cpaslkhfdp afsgecphcg
      481 erssnlyhiv wacpsnpaft pspnptredw eatllaccdl kaqkamvera maaaeatgvp
      541 y
//
LOCUS       XP_077523650             390 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134663 [Amblyomma americanum].
ACCESSION   XP_077523650
VERSION     XP_077523650.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667524.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..390
                     /product="uncharacterized protein LOC144134663"
                     /calculated_mol_wt=43723
     CDS             1..390
                     /gene="LOC144134663"
                     /coded_by="XM_077667524.1:120..1292"
                     /db_xref="GeneID:144134663"
ORIGIN      
        1 mrdcagavkl ayqnlnfdhp aniavsfdgt wltrghsshv cvgtvielft gyvldfvvls
       61 nfclgchcgl keddpefeaw qaehkcqknt sskpgemeve aarilfsrsl ekyglryvtm
      121 lcdgdshafn nlqeakaygy vqiqkedctn hvskrmgtal rnlvhkkken gpslsgkgkl
      181 tgelitkltm yygwalkthq gdiekmhnav latyhhvtst daetnhglcp qgqeswckyn
      241 qalvkneppp khrynlpkyv adalqpvfvr lsdrkllerc qrgkmqnsne slhsviwslv
      301 pktkqaslfa vqsavaepvt rvnagkeyat kailqqlsvn hgtaaiersa ekdrrwlats
      361 dakhlqaerf rsamkkrtte rdsdympgaf
//
LOCUS       XP_077523658             114 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134674 [Amblyomma americanum].
ACCESSION   XP_077523658
VERSION     XP_077523658.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667532.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..114
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..114
                     /product="uncharacterized protein LOC144134674"
                     /calculated_mol_wt=11721
     CDS             1..114
                     /gene="LOC144134674"
                     /coded_by="XM_077667532.1:335..679"
                     /db_xref="GeneID:144134674"
ORIGIN      
        1 mdvartrvrl rarppsrvcs ersplslela asleqrlafp ldpvgkqgav pggrdegrsk
       61 naggagveap ptcsrtppka aqaeepaagr nrkaggvsdi alleqsqvse aspq
//
LOCUS       XP_077523669             541 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134691 [Amblyomma americanum].
ACCESSION   XP_077523669
VERSION     XP_077523669.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667543.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..541
                     /product="uncharacterized protein LOC144134691"
                     /calculated_mol_wt=60249
     Region          <9..99
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Region          284..393
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     Site            order(288..291,319..320,323,342,379)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260008"
     CDS             1..541
                     /gene="LOC144134691"
                     /coded_by="XM_077667543.1:1..1626"
                     /db_xref="GeneID:144134691"
ORIGIN      
        1 mknlpvhlek vpgvqhalya ddisiwatqg slgemeaslq eaatavneya krcglhcspq
       61 ksefvhirpn kkctnkielr lgsepiperk eiralglfih qrrlatttld klkkvgdqvg
      121 rmvrrvsnkk gglrskdalr lanafvtsri lystpylrln khdqniievi irkmakkald
      181 lpittsnlrl qglgmtnsfd elreahltgq ytrlsqtpsg rrllarlhie httlteervr
      241 lsqewryalh vrplpinmsr ddhegrrrar aealahhygr kpgvyyvdas gphqggwyta
      301 avihesntvn gltfkarsvt qaeevaiala athsdsktit tdsrgacrnv qqgwvpylay
      361 rllqnssyvg gpshrfivwa pahsglggne aadaaarals nratsspass eevaeatpty
      421 tykeitqlyk drhklyphpc kgltraeerl flrlvtntmm cpaslkhfdp afsgecphcg
      481 erssnlyhiv wacpsnpaft pnpnptredw eatllaccdl kaqkamvera maaaeatgvp
      541 y
//
LOCUS       XP_077523696             673 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134722 [Amblyomma americanum].
ACCESSION   XP_077523696
VERSION     XP_077523696.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667570.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..673
                     /product="uncharacterized protein LOC144134722"
                     /calculated_mol_wt=75217
     Region          1..231
                     /region_name="RT_nLTR_like"
                     /note="RT_nLTR: Non-LTR (long terminal repeat)
                     retrotransposon and non-LTR retrovirus reverse
                     transcriptase (RT). This subfamily contains both non-LTR
                     retrotransposons and non-LTR retrovirus RTs. RTs catalyze
                     the conversion of single-stranded RNA into...; cd01650"
                     /db_xref="CDD:238827"
     Site            order(60..65,120..121,151,153..154,227..228)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238827"
     Site            order(60..65,120,153)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238827"
     Site            121
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238827"
     Region          416..525
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     Site            order(420..423,451..452,455,474,511)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260008"
     CDS             1..673
                     /gene="LOC144134722"
                     /coded_by="XM_077667570.1:117..2138"
                     /db_xref="GeneID:144134722"
ORIGIN      
        1 meamvrdrls eylenkrvfa dsmygfrpnk saqdillqls revlnpiehp ngdkivlald
       61 lkgafdnvth eailthlset gcgrnafeyi krfltdrqay vriqnaehgp fllgtrrtpq
      121 gavlspllfn lamknlpvhl ekvpgvqhal yaddisiwat qgslgemeas lqeaatavne
      181 yakrcglhcs pqksefvhir pnkkctnkie lrlgsepipe rkeiralglf ihqrrlattt
      241 ldklkkvgdq vgrmvrrvsn kkgglrskda lrlanafvts rilystpylr lnkhdqniie
      301 viirkmakka ldlpittsnl rlqglgmtns fdelreahlt gqytrlsqtp sgrrllarlh
      361 iehttlteer vrlsqewrya lhvrplpinm srddhegrrr araealahhy grkpgvyyvd
      421 asgphqggwy taavihesnt vngltfkars vtqaeevaia laathsdskt ittdsrgacr
      481 nvqqgwvpyl ayrllqnssy vggpshrfiv wapahsglgg neaadaaara lsnratsspa
      541 sseevaeatp tytykeitql ykdrhklyph pckgltraee rlflrlvtnt mmcpaslkhf
      601 dpafsgecph cgerssnlyh ivwacpsnpa ftpnpnptre dweatllacc dlkaqkamve
      661 ramaaaeatg vpy
//
LOCUS       XP_077523718             220 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134762 [Amblyomma americanum].
ACCESSION   XP_077523718
VERSION     XP_077523718.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667592.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..220
                     /product="uncharacterized protein LOC144134762"
                     /calculated_mol_wt=23922
     CDS             1..220
                     /gene="LOC144134762"
                     /coded_by="XM_077667592.1:85..747"
                     /db_xref="GeneID:144134762"
ORIGIN      
        1 mhgaslnvts seldvpaeat alatthdste taqvaparft gtedfvmhid elftslkamp
       61 lqrppstian asvayisgfv araveerrtc sfcpplhqsd gpspvlmgli hlqsrggltf
      121 pksefvtvlv tvkkavdial phikksnvrq qlaelllprl eqcplfacpa rddhaastls
      181 vvfdefmrpl lanvgatvke rtayrkklac kplhrkvrrv
//
LOCUS       XP_077523729             107 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134772 [Amblyomma americanum].
ACCESSION   XP_077523729
VERSION     XP_077523729.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667603.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..107
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..107
                     /product="uncharacterized protein LOC144134772"
                     /calculated_mol_wt=11622
     CDS             1..107
                     /gene="LOC144134772"
                     /coded_by="XM_077667603.1:200..523"
                     /db_xref="GeneID:144134772"
ORIGIN      
        1 mqarfiigtv ilvvlcttvd gcdhmgyrrd agrcktkhts nlqqagasdt ccafyvlsec
       61 lkrasqysdc pqayaqaelk elreasrlta dqcqggrelc geaprrg
//
LOCUS       XP_077523739             303 aa            linear   INV 25-NOV-2025
DEFINITION  ranBP-type and C3HC4-type zinc finger-containing protein 1-like
            [Amblyomma americanum].
ACCESSION   XP_077523739
VERSION     XP_077523739.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667613.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..303
                     /product="ranBP-type and C3HC4-type zinc finger-containing
                     protein 1-like"
                     /calculated_mol_wt=33410
     Region          37..87
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(39,44,60,63,68,71)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     Region          117..169
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(126,129,144,146,149,152,167)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          208..260
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(215,220,235,238,243,246)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     CDS             1..303
                     /gene="LOC144134781"
                     /coded_by="XM_077667613.1:93..1004"
                     /db_xref="GeneID:144134781"
ORIGIN      
        1 msvatrsagh lrldgdasqs tfpsektprn erlsplyrca kpgcsgswhw dgpatepvrc
       61 pqcsalncla craihdglsc eqhiarlvra mdsgaledsk ekkkrlqeql dlytkdivan
      121 sapftcpvck sdvakgdgac lknchhtvcr gcilgaidrs gtvavkcpys annivcdidi
      181 qdtevrallt reeyeeylqk gllevkvsak nffhcktidc pwwcfieasv peveckvcgt
      241 kncvncdaih lgmtcaeyqa wqasmkqlvp vrcaddmdnl lafmlhvaym aciiyllvkc
      301 vfp
//
LOCUS       XP_077523759             375 aa            linear   INV 25-NOV-2025
DEFINITION  forkhead box protein L1-like [Amblyomma americanum].
ACCESSION   XP_077523759
VERSION     XP_077523759.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667633.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..375
                     /product="forkhead box protein L1-like"
                     /calculated_mol_wt=40967
     Region          77..166
                     /region_name="FH_FOXL1"
                     /note="Forkhead (FH) domain found in Forkhead box protein
                     L1 (FOXL1) and similar proteins; cd20027"
                     /db_xref="CDD:410801"
     Site            order(82,100..101,104,123..124,126..127,130,137,147..149,
                     151)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410801"
     Region          222..>338
                     /region_name="PHA03269"
                     /note="envelope glycoprotein C; Provisional"
                     /db_xref="CDD:165527"
     CDS             1..375
                     /gene="LOC144134799"
                     /coded_by="XM_077667633.1:172..1299"
                     /db_xref="GeneID:144134799"
ORIGIN      
        1 mkssadcqgl ehggacnsdf fyppgclapv setfphclap pergggsvqf pispyyrpdv
       61 galsdiasla vaegamkppy syialitmai rsapeqkitl ngiykfimdr fpfyhhnkqg
      121 wqnsirhnls lndcfvklpr ekgkpgkghy wtlgadcesm fengnfrrrk rrkkqpalem
      181 erpdaavlak hdrlfasatg rpcheliglq aapvqlplpp ralrapeelv kpgrsrsppp
      241 gpayeaskad gqfnssftie nimkrpspaa sshtetdmpp llhvsrlaat agcggsffvp
      301 lpaftlplyl gkswfveggf aqmqrqpgvf wsplsrpeal tsnklllvsp dsqagcdafp
      361 pllqtdlgek rrvtp
//
LOCUS       XP_077523770             325 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase MARCHF3-like [Amblyomma americanum].
ACCESSION   XP_077523770
VERSION     XP_077523770.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667644.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..325
                     /product="E3 ubiquitin-protein ligase MARCHF3-like"
                     /calculated_mol_wt=36204
     Region          106..156
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(107,110,125,130,133,136,149,152)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     CDS             1..325
                     /gene="LOC144134808"
                     /coded_by="XM_077667644.1:135..1112"
                     /db_xref="GeneID:144134808"
ORIGIN      
        1 mrrdsrldai fllgtpllsl preavlirrf amsdrartaa tstprestpi lgrverrdqt
       61 pcadsfsasp ecrvhvpkay repgdeeedv plspaptnvt tssgpicric hegdhglpll
      121 siclcsgtmg lvhlfclehw lsasgsetce ichyrfnver rprrfcewlr sasvrrgllg
      181 dvmcfgllsp laflcgvlcl hgaaqqmlqq rlwesigltt lafllftvys awavltfryh
      241 yrswrkwrea npyvkvvdap rqfqdlpena gkktevtvph vavrlplagr stlpsgsgld
      301 ssfelyspnt vasgstawey ctsrl
//
LOCUS       XP_077523781            1170 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134825 [Amblyomma americanum].
ACCESSION   XP_077523781
VERSION     XP_077523781.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667655.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1170
                     /product="uncharacterized protein LOC144134825"
                     /calculated_mol_wt=131172
     Region          <25..118
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          31..60
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(34..35,38..40,42..43,47,50,60,62,64,68..69,72..74,
                     76..77,81,84,93,95,97,101..102,105..107,109..110,114)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          62..93
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          789..1044
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Region          1050..1170
                     /region_name="RNase_Ire1_like"
                     /note="RNase domain (also known as the kinase extension
                     nuclease domain) of Ire1 and RNase L; cl15368"
                     /db_xref="CDD:472796"
     CDS             1..1170
                     /gene="LOC144134825"
                     /coded_by="XM_077667655.1:20..3532"
                     /db_xref="GeneID:144134825"
ORIGIN      
        1 mmdtlvfhel fnkleklgra rslmtdksnk tllhaaaeig wdygvafllr rsktdmeqks
       61 kdgrtpmmca aaegrteavg lllraganil sedqsgvtaa claicrhhda avkaifnkms
      121 saekeqyvsr rislllspts sardyrcfyr dtlfkvllfi rrhleggtel llshdvftsl
      181 vscgrrcrnd psvvgpmfhl caclmhdkad yskyvhrdmv eafmssggle laldvlghet
      241 dscefqsrfa allpvllvae veeglrwmre hlddlvrpyr efsskkdlla ysadgervkt
      301 nvmwerfset lghlytearg kllnsgspvg dqpahgtnfa llqgtavkrk sppqsirsck
      361 tvgkkitvrp ravpamlqvt fnddsrmpkk vrrtqqclqr pevkiiatkp mnvlevmsra
      421 ptvvptalpl qgyanalktn lpkpepsfll eearpprfkf ttegvlsprk trdlsfpana
      481 hksstdgyss qpcsisadad nglvrraqsd ptlsspkekt gqklklayle pnafgksvln
      541 escelcrthr taqallipme tetscefshk raasdddass mekedehdgr sleggnssds
      601 dhgdrwaipg qskhklqckk lhyfqehlfr eiqrvrktek ksdtalaami laetmlrsmn
      661 sltlkpayka ncsldprvva qplytldggt rdlsteqndg epslsndpac spadgawsgn
      721 fasvacreia ydskanlssv wlsesqrwkn afadvanapl sslvaangff yserkknfla
      781 gvryyentsv hlgiakngre vivkkvqhhw geyehvvesv rsfkkidlfh gninefhvtl
      841 qkslahvyvl whpqdrnldq ymmmlrerkl slvkwgrmam aqlldalafl htrekpvvhg
      901 rlkpsnilis prgnillsdl aprgalpsvp cgfimtdmyq ssrgqitdas wrprelivam
      961 tqefaavvft tasdmqvagm liyymltgah pfgkcdddcq lnilegrvml kpvnselddl
     1021 vhhllamepe yrlsatgair hpvfwgprkr lfflsavale mehrkderit egiihqlnis
     1081 ckcsnwtarv ppqviqdafp egnppdeats llklikrctl eydamsaaar nalkqperff
     1141 veafpsllmt vhgvvrrihw ryspsveaff
//
LOCUS       XP_077523789             379 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134849 [Amblyomma americanum].
ACCESSION   XP_077523789
VERSION     XP_077523789.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667663.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..379
                     /product="uncharacterized protein LOC144134849"
                     /calculated_mol_wt=42225
     Region          125..199
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          301..343
                     /region_name="EB"
                     /note="EB module; pfam01683"
                     /db_xref="CDD:460294"
     CDS             1..379
                     /gene="LOC144134849"
                     /coded_by="XM_077667663.1:296..1435"
                     /db_xref="GeneID:144134849"
ORIGIN      
        1 mlslrfsvll pllvapsgga rwawgprael sqflqnmswq hdphiseelr ksvlgilgrq
       61 pspgllhrrr vpfrpigelp vfgslagrvs rsprsrtdhh sglpvggsil taknrpqnmk
      121 fergkpniyd vhmgksvqlf csvayqsqvs ifwtlngkpl edydslseet vpegrvssvl
      181 iksvtrlptm tgvytfnctt ltdydlatvh ldvnmplsda cqdtstecad rmavcknsac
      241 vcegyykvrl lskhvtcrse ayletpcmyd eqclnttehs ectghgwcac lkgygrteeh
      301 kcvpkvgars rcnseeecad ydatcilnhc tcfshkkeln drcvnlaehl eanklpygrg
      361 vrrspvkllv vlvallrfa
//
LOCUS       XP_077523797             170 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134857 [Amblyomma americanum].
ACCESSION   XP_077523797
VERSION     XP_077523797.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667671.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..170
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..170
                     /product="uncharacterized protein LOC144134857"
                     /calculated_mol_wt=18392
     CDS             1..170
                     /gene="LOC144134857"
                     /coded_by="XM_077667671.1:24..536"
                     /db_xref="GeneID:144134857"
ORIGIN      
        1 mvtvsgspvs tipepswefg strrrmftts qapdsdyagt hvgsapvlnv geqlshgepp
       61 tkktpggetn prgpktsglf rlfariqnel rtrcgpkrsg rrgvcamavg rfsklaswih
      121 glwlarkpsv dgpeiydlyn tlhsdeewst sltaaevslq gttifkdvaf
//
LOCUS       XP_077523813             105 aa            linear   INV 25-NOV-2025
DEFINITION  THAP domain-containing protein 11-like [Amblyomma americanum].
ACCESSION   XP_077523813
VERSION     XP_077523813.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667687.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..105
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..105
                     /product="THAP domain-containing protein 11-like"
                     /calculated_mol_wt=11976
     Region          4..79
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..105
                     /gene="LOC144134871"
                     /coded_by="XM_077667687.1:125..442"
                     /db_xref="GeneID:144134871"
ORIGIN      
        1 mpgccvplct nhsrngwkmy hfptepkrrl lwiakvkrdk wqptkgscvc nahfeennfe
       61 qnradgwkkl kpnavptvfp frgnfapeke atkrkdgtsg aiwgm
//
LOCUS       XP_077523819             146 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134882 [Amblyomma americanum].
ACCESSION   XP_077523819
VERSION     XP_077523819.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667693.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..146
                     /product="uncharacterized protein LOC144134882"
                     /calculated_mol_wt=14459
     CDS             1..146
                     /gene="LOC144134882"
                     /coded_by="XM_077667693.1:24..464"
                     /db_xref="GeneID:144134882"
ORIGIN      
        1 maphvcnpmc licggphlts srectakfik lpqpgprtps kgansgatrg grqqnnqktp
       61 rrgvmpppgk tatvtpppga aefpalgasp ggaacnqpkr glgggparls rpeepkglgs
      121 tdaggvacqw gpgigappst vrgkgq
//
LOCUS       XP_077523827             108 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134894 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077523827
VERSION     XP_077523827.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667701.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..108
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..108
                     /product="uncharacterized protein LOC144134894 isoform X1"
                     /calculated_mol_wt=11837
     CDS             1..108
                     /gene="LOC144134894"
                     /coded_by="XM_077667701.1:156..482"
                     /db_xref="GeneID:144134894"
ORIGIN      
        1 maaanlesgp wkkcfqgtvs tyakaianii paetlatnfv hwsrcysatc fihfcvqkva
       61 nrlgmwrsai sclvqhricg vprqshtyqn daaasaftay gcdmqdeq
//
LOCUS       XP_077523836              98 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134894 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077523836
VERSION     XP_077523836.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667710.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..98
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..98
                     /product="uncharacterized protein LOC144134894 isoform X2"
                     /calculated_mol_wt=10687
     CDS             1..98
                     /gene="LOC144134894"
                     /coded_by="XM_077667710.1:156..452"
                     /db_xref="GeneID:144134894"
ORIGIN      
        1 maaanlesgp wkkcfqgtvs tyakaianii paetlatnfv hwsrcysatc fihfcvqkts
       61 sdvsrkaisv sssaaerrcc knilqehmga tgkewdir
//
LOCUS       XP_077523842             136 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134906 [Amblyomma americanum].
ACCESSION   XP_077523842
VERSION     XP_077523842.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667716.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..136
                     /product="uncharacterized protein LOC144134906"
                     /calculated_mol_wt=12671
     CDS             1..136
                     /gene="LOC144134906"
                     /coded_by="XM_077667716.1:120..530"
                     /db_xref="GeneID:144134906"
ORIGIN      
        1 mvsalslvta aacicvvlcd dghgiykkfp paaftigfgh dhglkiahgg lkvgvgfggg
       61 glglgllhrv ggyggggygg gggyggghgg ghgisigllg hginlghggg ygggygggyg
      121 ggygggyggg yghgww
//
LOCUS       XP_077523849             260 aa            linear   INV 25-NOV-2025
DEFINITION  homeobox protein MSX-2-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077523849
VERSION     XP_077523849.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667723.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..260
                     /product="homeobox protein MSX-2-like isoform X1"
                     /calculated_mol_wt=27305
     Region          126..182
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..260
                     /gene="LOC144134915"
                     /coded_by="XM_077667723.1:84..866"
                     /db_xref="GeneID:144134915"
ORIGIN      
        1 msspgpaggt papaspetra atptkpspfs vaslladtrp sgqlrwtapa faagilhaap
       61 rdpglpflhg pvagggpfgw alhapggtqp cgpswfglap hhalpkpgev pslpvkcqlr
      121 rhksnrkprt pfttqqllal erkfrvkqyl siaeraefss slnltetqvk iwfqnrrake
      181 krlkeaelek lrmamrpllp gafglglaag pfggtagasg avpggymhpp rlmqasppah
      241 hppfggaeav salpqqrpal
//
LOCUS       XP_077523855             260 aa            linear   INV 25-NOV-2025
DEFINITION  homeobox protein MSX-2-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077523855
VERSION     XP_077523855.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667729.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..260
                     /product="homeobox protein MSX-2-like isoform X2"
                     /calculated_mol_wt=27305
     Region          126..182
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..260
                     /gene="LOC144134915"
                     /coded_by="XM_077667729.1:84..866"
                     /db_xref="GeneID:144134915"
ORIGIN      
        1 msspgpaggt papaspetra atptkpspfs vaslladtrp sgqlrwtapa faagilhaap
       61 rdpglpflhg pvagggpfgw alhapggtqp cgpswfglap hhalpkpgev pslpvkcqlr
      121 rhksnrkprt pfttqqllal erkfrvkqyl siaeraefss slnltetqvk iwfqnrrake
      181 krlkeaelek lrmamrpllp gafglglaag pfggtagasg avpggymhpp rlmqasppah
      241 hppfggaeav salpqqrpal
//
LOCUS       XP_077523861             234 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144134915 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077523861
VERSION     XP_077523861.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667735.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..234
                     /product="uncharacterized protein LOC144134915 isoform X3"
                     /calculated_mol_wt=23365
     CDS             1..234
                     /gene="LOC144134915"
                     /coded_by="XM_077667735.1:84..788"
                     /db_xref="GeneID:144134915"
ORIGIN      
        1 msspgpaggt papaspetra atptkpspfs vaslladtrp sgqlrwtapa faagilhaap
       61 rdpglpflhg pvagggpfgw alhapggtqp cgpswfglap hhalpsedlv peparqgeaa
      121 qgsragevah ghaaaatgrl rsgpgrgsvr rngrclgrca rrlhapsaph agiaaraspt
      181 frrrrgrvgl aataarlvst saaapgraac avtpspglrd ggreghtgsv qyiq
//
LOCUS       XP_077523867             274 aa            linear   INV 25-NOV-2025
DEFINITION  aurora kinase A-A-like [Amblyomma americanum].
ACCESSION   XP_077523867
VERSION     XP_077523867.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667741.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..274
                     /product="aurora kinase A-A-like"
                     /calculated_mol_wt=31896
     Region          10..262
                     /region_name="STKc_Aurora"
                     /note="Catalytic domain of the Serine/Threonine kinase,
                     Aurora kinase; cd14007"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,88..91,95,97,134,136,138..139,
                     141,152,155,169..172)
                     /site_type="active"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,89,91,95,138..139,141,152)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270909"
     Site            order(21,95,97,134,136,138,155,169..172)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270909"
     Site            order(151..160,163..172)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270909"
     CDS             1..274
                     /gene="LOC144134951"
                     /coded_by="XM_077667741.1:220..1044"
                     /db_xref="GeneID:144134951"
ORIGIN      
        1 msvkewclkd fqigrplgkg kfgrvylare ketkfivalk vmfksqlqrn nvehqlrrei
       61 eiqshlrhpn ilclynwfhd esrvyliley apqgelyrhl tregrftdqr aatyiyqlcn
      121 alkvchslnv ihrdikpenl llgingeiki adfgwsvhap fsrratrcgt ldylapeive
      181 givydekvdh walgiiiyef lvgtppfese trqetyrrir dgrvqfppyv cadarniivn
      241 llkirpadri sldevlshpw vttnadtsie cktl
//
LOCUS       XP_077523876             274 aa            linear   INV 25-NOV-2025
DEFINITION  aurora kinase A-A-like [Amblyomma americanum].
ACCESSION   XP_077523876
VERSION     XP_077523876.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667750.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..274
                     /product="aurora kinase A-A-like"
                     /calculated_mol_wt=31896
     Region          10..262
                     /region_name="STKc_Aurora"
                     /note="Catalytic domain of the Serine/Threonine kinase,
                     Aurora kinase; cd14007"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,88..91,95,97,134,136,138..139,
                     141,152,155,169..172)
                     /site_type="active"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,89,91,95,138..139,141,152)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270909"
     Site            order(21,95,97,134,136,138,155,169..172)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270909"
     Site            order(151..160,163..172)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270909"
     CDS             1..274
                     /gene="LOC144134959"
                     /coded_by="XM_077667750.1:564..1388"
                     /db_xref="GeneID:144134959"
ORIGIN      
        1 msvkewclkd fqigrplgkg kfgrvylare ketkfivalk vmfksqlqrn nvehqlrrei
       61 eiqshlrhpn ilclynwfhd esrvyliley apqgelyrhl tregrftdqr aatyiyqlcn
      121 alkvchslnv ihrdikpenl llgingeiki adfgwsvhap fsrratrcgt ldylapeive
      181 givydekvdh walgiiiyef lvgtppfese trqetyrrir dgrvqfppyv cadarniivn
      241 llkirpadri sldevlshpw vttnadtsie cktl
//
LOCUS       XP_077523886             274 aa            linear   INV 25-NOV-2025
DEFINITION  aurora kinase A-A-like [Amblyomma americanum].
ACCESSION   XP_077523886
VERSION     XP_077523886.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667760.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..274
                     /product="aurora kinase A-A-like"
                     /calculated_mol_wt=31915
     Region          10..262
                     /region_name="STKc_Aurora"
                     /note="Catalytic domain of the Serine/Threonine kinase,
                     Aurora kinase; cd14007"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,88..91,95,97,134,136,138..139,
                     141,152,155,169..172)
                     /site_type="active"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,89,91,95,138..139,141,152)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270909"
     Site            order(21,95,97,134,136,138,155,169..172)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270909"
     Site            order(151..160,163..172)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270909"
     CDS             1..274
                     /gene="LOC144134971"
                     /coded_by="XM_077667760.1:584..1408"
                     /db_xref="GeneID:144134971"
ORIGIN      
        1 msvkewclkd feigrplgkg kfgrvylare ketkfivalk vmfksqlqkn nvehqlrrei
       61 eiqshlrhpn ilclynwfhd esrvyliley apqgelyrhl tregrftdqr aatyiyqlcn
      121 alkvchslnv ihrdikpenl llgingeiki adfgwsvhtp ysrratrcgt ldylapeive
      181 givydekvdh walgiiiyef lvgtppfese trqetyrrir dgrvqfppyv cadarniivn
      241 llkirpadri sldevlshpw vttnadtsie cktl
//
LOCUS       XP_077523894             274 aa            linear   INV 25-NOV-2025
DEFINITION  aurora kinase A-A-like [Amblyomma americanum].
ACCESSION   XP_077523894
VERSION     XP_077523894.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667768.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..274
                     /product="aurora kinase A-A-like"
                     /calculated_mol_wt=31755
     Region          10..262
                     /region_name="STKc_Aurora"
                     /note="Catalytic domain of the Serine/Threonine kinase,
                     Aurora kinase; cd14007"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,88..91,95,97,134,136,138..139,
                     141,152,155,169..172)
                     /site_type="active"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,89,91,95,138..139,141,152)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270909"
     Site            order(21,95,97,134,136,138,155,169..172)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270909"
     Site            order(151..160,163..172)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270909"
     CDS             1..274
                     /gene="LOC144134982"
                     /coded_by="XM_077667768.1:566..1390"
                     /db_xref="GeneID:144134982"
ORIGIN      
        1 msvkewclkd fqigrplgkg kfgrvylare ketkfivalk vmfksqlqkn nvehqlrrei
       61 eiqshlrhpn ilclynwfhd esrvyliley apqgelyrhl tregrftdqr aatyiyqlcn
      121 alkvchslnv ihrdikpenl llgingeiki adfglsvhap fsrratrcgt ldylapeive
      181 gtaydekvdh walgiiiyef lvgtppfese trqetyrrir dgrvqfppyv cadarniivn
      241 llkirpadri sldevlshpw vttnadtsie cktl
//
LOCUS       XP_077523900             274 aa            linear   INV 25-NOV-2025
DEFINITION  aurora kinase A-A-like [Amblyomma americanum].
ACCESSION   XP_077523900
VERSION     XP_077523900.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667774.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..274
                     /product="aurora kinase A-A-like"
                     /calculated_mol_wt=31755
     Region          10..262
                     /region_name="STKc_Aurora"
                     /note="Catalytic domain of the Serine/Threonine kinase,
                     Aurora kinase; cd14007"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,88..91,95,97,134,136,138..139,
                     141,152,155,169..172)
                     /site_type="active"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,89,91,95,138..139,141,152)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270909"
     Site            order(21,95,97,134,136,138,155,169..172)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270909"
     Site            order(151..160,163..172)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270909"
     CDS             1..274
                     /gene="LOC144134982"
                     /coded_by="XM_077667774.1:546..1370"
                     /db_xref="GeneID:144134982"
ORIGIN      
        1 msvkewclkd fqigrplgkg kfgrvylare ketkfivalk vmfksqlqkn nvehqlrrei
       61 eiqshlrhpn ilclynwfhd esrvyliley apqgelyrhl tregrftdqr aatyiyqlcn
      121 alkvchslnv ihrdikpenl llgingeiki adfglsvhap fsrratrcgt ldylapeive
      181 gtaydekvdh walgiiiyef lvgtppfese trqetyrrir dgrvqfppyv cadarniivn
      241 llkirpadri sldevlshpw vttnadtsie cktl
//
LOCUS       XP_077523907             274 aa            linear   INV 25-NOV-2025
DEFINITION  aurora kinase A-A-like [Amblyomma americanum].
ACCESSION   XP_077523907
VERSION     XP_077523907.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667781.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..274
                     /product="aurora kinase A-A-like"
                     /calculated_mol_wt=31828
     Region          10..262
                     /region_name="STKc_Aurora"
                     /note="Catalytic domain of the Serine/Threonine kinase,
                     Aurora kinase; cd14007"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,88..91,95,97,134,136,138..139,
                     141,152,155,169..172)
                     /site_type="active"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,89,91,95,138..139,141,152)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270909"
     Site            order(21,95,97,134,136,138,155,169..172)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270909"
     Site            order(151..160,163..172)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270909"
     CDS             1..274
                     /gene="LOC144134997"
                     /coded_by="XM_077667781.1:548..1372"
                     /db_xref="GeneID:144134997"
ORIGIN      
        1 msvkewclkd fqigrplgkg kfgrvylare ketkfivalk vmfksqlqkn nvehqlrrei
       61 eiqshlrhpn ilclynwfhd esrvyliley apqgelyrhl tregrftdqr aatyiyqlcn
      121 alkvchslnv ihrdikpenl llgingeiki adfgwsvhap fsrratrcgt ldylapeive
      181 gtaydekvdh walgiiiyef lvgtppfese trqetyrrir dgrvqfppyv cadarniivn
      241 llkirpadri sldevlshpw vttnadtsie cktl
//
LOCUS       XP_077523912             274 aa            linear   INV 25-NOV-2025
DEFINITION  aurora kinase A-A-like [Amblyomma americanum].
ACCESSION   XP_077523912
VERSION     XP_077523912.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667786.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..274
                     /product="aurora kinase A-A-like"
                     /calculated_mol_wt=31828
     Region          10..262
                     /region_name="STKc_Aurora"
                     /note="Catalytic domain of the Serine/Threonine kinase,
                     Aurora kinase; cd14007"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,88..91,95,97,134,136,138..139,
                     141,152,155,169..172)
                     /site_type="active"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,89,91,95,138..139,141,152)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270909"
     Site            order(21,95,97,134,136,138,155,169..172)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270909"
     Site            order(151..160,163..172)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270909"
     CDS             1..274
                     /gene="LOC144134997"
                     /coded_by="XM_077667786.1:528..1352"
                     /db_xref="GeneID:144134997"
ORIGIN      
        1 msvkewclkd fqigrplgkg kfgrvylare ketkfivalk vmfksqlqkn nvehqlrrei
       61 eiqshlrhpn ilclynwfhd esrvyliley apqgelyrhl tregrftdqr aatyiyqlcn
      121 alkvchslnv ihrdikpenl llgingeiki adfgwsvhap fsrratrcgt ldylapeive
      181 gtaydekvdh walgiiiyef lvgtppfese trqetyrrir dgrvqfppyv cadarniivn
      241 llkirpadri sldevlshpw vttnadtsie cktl
//
LOCUS       XP_077523919             274 aa            linear   INV 25-NOV-2025
DEFINITION  aurora kinase A-A-like [Amblyomma americanum].
ACCESSION   XP_077523919
VERSION     XP_077523919.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667793.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..274
                     /product="aurora kinase A-A-like"
                     /calculated_mol_wt=31884
     Region          10..262
                     /region_name="STKc_Aurora"
                     /note="Catalytic domain of the Serine/Threonine kinase,
                     Aurora kinase; cd14007"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,88..91,95,97,134,136,138..139,
                     141,152,155,169..172)
                     /site_type="active"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,89,91,95,138..139,141,152)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270909"
     Site            order(21,95,97,134,136,138,155,169..172)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270909"
     Site            order(151..160,163..172)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270909"
     CDS             1..274
                     /gene="LOC144135016"
                     /coded_by="XM_077667793.1:281..1105"
                     /db_xref="GeneID:144135016"
ORIGIN      
        1 msvkewclkd fqigrplgkg kfgrvylare ketkfivalk vmfksqlqrn nvehqlrrei
       61 eiqshlrhpn ilclynwfhd esrvyliley apqgelyrhl tregrftdqr aatyiyqlcn
      121 alkvchslnv ihrdikpenl llgingeiki adfgwsvhap fsrratrcgt ldylapeive
      181 gtvydekvdh walgiiiyef lvgtppfese trqetyrrir dgrvqfppyv cadarniivn
      241 llkirpadri sldevlshpw vttnadtsie cktl
//
LOCUS       XP_077523924             274 aa            linear   INV 25-NOV-2025
DEFINITION  aurora kinase A-A-like [Amblyomma americanum].
ACCESSION   XP_077523924
VERSION     XP_077523924.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667798.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..274
                     /product="aurora kinase A-A-like"
                     /calculated_mol_wt=31884
     Region          10..262
                     /region_name="STKc_Aurora"
                     /note="Catalytic domain of the Serine/Threonine kinase,
                     Aurora kinase; cd14007"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,88..91,95,97,134,136,138..139,
                     141,152,155,169..172)
                     /site_type="active"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,89,91,95,138..139,141,152)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270909"
     Site            order(21,95,97,134,136,138,155,169..172)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270909"
     Site            order(151..160,163..172)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270909"
     CDS             1..274
                     /gene="LOC144135016"
                     /coded_by="XM_077667798.1:261..1085"
                     /db_xref="GeneID:144135016"
ORIGIN      
        1 msvkewclkd fqigrplgkg kfgrvylare ketkfivalk vmfksqlqrn nvehqlrrei
       61 eiqshlrhpn ilclynwfhd esrvyliley apqgelyrhl tregrftdqr aatyiyqlcn
      121 alkvchslnv ihrdikpenl llgingeiki adfgwsvhap fsrratrcgt ldylapeive
      181 gtvydekvdh walgiiiyef lvgtppfese trqetyrrir dgrvqfppyv cadarniivn
      241 llkirpadri sldevlshpw vttnadtsie cktl
//
LOCUS       XP_077523934             274 aa            linear   INV 25-NOV-2025
DEFINITION  aurora kinase A-A-like [Amblyomma americanum].
ACCESSION   XP_077523934
VERSION     XP_077523934.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667808.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..274
                     /product="aurora kinase A-A-like"
                     /calculated_mol_wt=31856
     Region          10..262
                     /region_name="STKc_Aurora"
                     /note="Catalytic domain of the Serine/Threonine kinase,
                     Aurora kinase; cd14007"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,88..91,95,97,134,136,138..139,
                     141,152,155,169..172)
                     /site_type="active"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,89,91,95,138..139,141,152)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270909"
     Site            order(21,95,97,134,136,138,155,169..172)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270909"
     Site            order(151..160,163..172)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270909"
     CDS             1..274
                     /gene="LOC144135034"
                     /coded_by="XM_077667808.1:281..1105"
                     /db_xref="GeneID:144135034"
ORIGIN      
        1 msvkewclkd fqigrplgkg kfgrvylare ketkfivalk vmfksqlqrn nvehqlrrei
       61 eiqshlrhpn ilclynwfhd esrvyliley apqgelyrhl tregrftdqr aatyiyqlcn
      121 alkvchslnv ihrdikpenl llgingeiki adfgwsvhap fsrratrcgt ldylapeive
      181 gtaydekvdh walgiiiyef lvgtppfese trqetyrrir dgrvqfppyv cadarniivn
      241 llkirpadri sldevlshpw vttnadtsie cktl
//
LOCUS       XP_077523935             274 aa            linear   INV 25-NOV-2025
DEFINITION  aurora kinase A-A-like [Amblyomma americanum].
ACCESSION   XP_077523935
VERSION     XP_077523935.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667809.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..274
                     /product="aurora kinase A-A-like"
                     /calculated_mol_wt=31856
     Region          10..262
                     /region_name="STKc_Aurora"
                     /note="Catalytic domain of the Serine/Threonine kinase,
                     Aurora kinase; cd14007"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,88..91,95,97,134,136,138..139,
                     141,152,155,169..172)
                     /site_type="active"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,89,91,95,138..139,141,152)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270909"
     Site            order(21,95,97,134,136,138,155,169..172)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270909"
     Site            order(151..160,163..172)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270909"
     CDS             1..274
                     /gene="LOC144135034"
                     /coded_by="XM_077667809.1:261..1085"
                     /db_xref="GeneID:144135034"
ORIGIN      
        1 msvkewclkd fqigrplgkg kfgrvylare ketkfivalk vmfksqlqrn nvehqlrrei
       61 eiqshlrhpn ilclynwfhd esrvyliley apqgelyrhl tregrftdqr aatyiyqlcn
      121 alkvchslnv ihrdikpenl llgingeiki adfgwsvhap fsrratrcgt ldylapeive
      181 gtaydekvdh walgiiiyef lvgtppfese trqetyrrir dgrvqfppyv cadarniivn
      241 llkirpadri sldevlshpw vttnadtsie cktl
//
LOCUS       XP_077523938             274 aa            linear   INV 25-NOV-2025
DEFINITION  aurora kinase A-A-like [Amblyomma americanum].
ACCESSION   XP_077523938
VERSION     XP_077523938.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667812.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..274
                     /product="aurora kinase A-A-like"
                     /calculated_mol_wt=31742
     Region          10..262
                     /region_name="STKc_Aurora"
                     /note="Catalytic domain of the Serine/Threonine kinase,
                     Aurora kinase; cd14007"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,88..91,95,97,134,136,138..139,
                     141,152,155,169..172)
                     /site_type="active"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,89,91,95,138..139,141,152)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270909"
     Site            order(21,95,97,134,136,138,155,169..172)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270909"
     Site            order(151..160,163..172)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270909"
     CDS             1..274
                     /gene="LOC144135048"
                     /coded_by="XM_077667812.1:565..1389"
                     /db_xref="GeneID:144135048"
ORIGIN      
        1 msvkewclkd feigrplgkg nfgrvylare ketkfivalk vmfksqlqkn nvehqlrrei
       61 eiqshlrhpn ilclynwfhd esrvyliley apqgelyrhl tregrftdqr aatyiyqlcn
      121 alkvchslnv ihrdikpenl llgingeiki adfglsvhap fsrratrcgt ldylapeive
      181 gtaydekvdh walgiiiyef lvgtppfese trqetyrrir dgrvqfppyv cadarniivn
      241 llkirpadri sldevlshpw vttnadtsie cktl
//
LOCUS       XP_077523942             274 aa            linear   INV 25-NOV-2025
DEFINITION  aurora kinase A-A-like [Amblyomma americanum].
ACCESSION   XP_077523942
VERSION     XP_077523942.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667816.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..274
                     /product="aurora kinase A-A-like"
                     /calculated_mol_wt=31742
     Region          10..262
                     /region_name="STKc_Aurora"
                     /note="Catalytic domain of the Serine/Threonine kinase,
                     Aurora kinase; cd14007"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,88..91,95,97,134,136,138..139,
                     141,152,155,169..172)
                     /site_type="active"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,89,91,95,138..139,141,152)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270909"
     Site            order(21,95,97,134,136,138,155,169..172)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270909"
     Site            order(151..160,163..172)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270909"
     CDS             1..274
                     /gene="LOC144135048"
                     /coded_by="XM_077667816.1:545..1369"
                     /db_xref="GeneID:144135048"
ORIGIN      
        1 msvkewclkd feigrplgkg nfgrvylare ketkfivalk vmfksqlqkn nvehqlrrei
       61 eiqshlrhpn ilclynwfhd esrvyliley apqgelyrhl tregrftdqr aatyiyqlcn
      121 alkvchslnv ihrdikpenl llgingeiki adfglsvhap fsrratrcgt ldylapeive
      181 gtaydekvdh walgiiiyef lvgtppfese trqetyrrir dgrvqfppyv cadarniivn
      241 llkirpadri sldevlshpw vttnadtsie cktl
//
LOCUS       XP_077523968             108 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135082 [Amblyomma americanum].
ACCESSION   XP_077523968
VERSION     XP_077523968.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667842.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..108
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..108
                     /product="uncharacterized protein LOC144135082"
                     /calculated_mol_wt=11346
     CDS             1..108
                     /gene="LOC144135082"
                     /coded_by="XM_077667842.1:34..360"
                     /db_xref="GeneID:144135082"
ORIGIN      
        1 msgvssgapa cpvagqrgtr rappicprft tapvsrkvle ptvyvvynii fadialhnas
       61 rrcswhrsfp phgaspyeag nknspqspcl eplgtaastv itsfilrg
//
LOCUS       XP_077524001             191 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135124 [Amblyomma americanum].
ACCESSION   XP_077524001
VERSION     XP_077524001.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667875.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..191
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..191
                     /product="uncharacterized protein LOC144135124"
                     /calculated_mol_wt=22193
     CDS             1..191
                     /gene="LOC144135124"
                     /coded_by="XM_077667875.1:117..692"
                     /db_xref="GeneID:144135124"
ORIGIN      
        1 mpgesnkaag dtvgtstlgk ddnmseasan kqqspsslkr hlrktygrrp melvsryfrs
       61 mfdlalyann aaflmrcram rvvprtyrve crdinntrhv vrildecsyr lmladldynr
      121 lrkvqvsrli erlheklqki ispedlrkvv vmakaryeni featrdkqra mfadllkeye
      181 iepkktgenq e
//
LOCUS       XP_077524011             207 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135132 [Amblyomma americanum].
ACCESSION   XP_077524011
VERSION     XP_077524011.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667885.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..207
                     /product="uncharacterized protein LOC144135132"
                     /calculated_mol_wt=23523
     CDS             1..207
                     /gene="LOC144135132"
                     /coded_by="XM_077667885.1:302..925"
                     /db_xref="GeneID:144135132"
ORIGIN      
        1 myakrdvptl dsvlkavsed ddlpnlkktt lwmlmkdvgf tfekrkrnla liersdiiaw
       61 rriylraike frsqgrciiy lhetwidagh ikeyvwrdkt vkssqdaflk glttelaaps
      121 gkgarlilvh vggsatgfvr daagffrakk rkhadyysei ddyfescftd klftnipani
      181 tvvmvnapyh ssaleksptk sprkadi
//
LOCUS       XP_077524015             540 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135145 [Amblyomma americanum].
ACCESSION   XP_077524015
VERSION     XP_077524015.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667889.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..540
                     /product="uncharacterized protein LOC144135145"
                     /calculated_mol_wt=60341
     Region          <9..99
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Region          284..394
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..540
                     /gene="LOC144135145"
                     /coded_by="XM_077667889.1:240..1862"
                     /db_xref="GeneID:144135145"
ORIGIN      
        1 mahlppllnd vpgirhslya dditlwathg slgqieanlk qaativdgya rrcglecspt
       61 ksefvhlrpn pkcttkiylt lasgpipehd evrvlgllin rnrkidttlq klrkigdqvg
      121 rmvrrvsnkr gglrckdalr lanafvtsri lysapylylr kcdenalevi lrkiykrald
      181 llittsnqrl idvgmtntfg elreahlnnq yvrlnkssag rsllhrlhia ytaqaeertn
      241 vpedwrralq vqplpqnmtk ethegrriar atalndrygs rygvfytdaa gphhggwyta
      301 aviyqnthvq gltfrapnit haeevaiala aaepdsrvii tdsrgacrnl kqgrisrraa
      361 kllhrceyrd rpetraiiwt pahagldgne aadassraln hrasprllpp evsepnpait
      421 fkevvqlyqt qhsrfhppak gltkieerwl irlysntvlc pavlqhfnpa fngecphcgc
      481 afsdvhhmvw acpsnpaypf fpstnreswe aallgcstle sqralvarar aaaeatgvpd
//
LOCUS       XP_077524020             325 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135152 [Amblyomma americanum].
ACCESSION   XP_077524020
VERSION     XP_077524020.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667894.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..325
                     /product="uncharacterized protein LOC144135152"
                     /calculated_mol_wt=35458
     CDS             1..325
                     /gene="LOC144135152"
                     /coded_by="XM_077667894.1:103..1080"
                     /db_xref="GeneID:144135152"
ORIGIN      
        1 mtphhavrwe patteafpep cvaasncqra lvavgfcaga qgcaprrrvr vlleiaavrs
       61 starhlnknk dnqprstwrq flrrygrspe gqrlyacdrw galiseilks ccgaaqetql
      121 camlyhiklp fpegtegstg qphhvfvtga eaehrvilvh trrvtvlvar vlyrrrcead
      181 ptvllvldha aspfptiptn lvldvpgala scvfntidda elavlqedla qdaahvicps
      241 adrvgrtppa lasrvmrtik gaeevailtq dlaqdaagaf hptetlmvrl lplsrlpslt
      301 pektrknfct sgwlsailpl vsltf
//
LOCUS       XP_077524031             113 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135162 [Amblyomma americanum].
ACCESSION   XP_077524031
VERSION     XP_077524031.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667905.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..113
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..113
                     /product="uncharacterized protein LOC144135162"
                     /calculated_mol_wt=12046
     CDS             1..113
                     /gene="LOC144135162"
                     /coded_by="XM_077667905.1:109..450"
                     /db_xref="GeneID:144135162"
ORIGIN      
        1 mplegfrcsg vrsipvalvp kddgsikinn pgpvraalqa atslyeaite lhsvssglyk
       61 rfgswcglll vssvqdtrll nllllncqks vllagafnsh hvswgfrtdf cgs
//
LOCUS       XP_077524039             317 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135173 [Amblyomma americanum].
ACCESSION   XP_077524039
VERSION     XP_077524039.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667913.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..317
                     /product="uncharacterized protein LOC144135173"
                     /calculated_mol_wt=34186
     CDS             1..317
                     /gene="LOC144135173"
                     /coded_by="XM_077667913.1:80..1033"
                     /db_xref="GeneID:144135173"
ORIGIN      
        1 mtphhavrkk patpaafpeg cvatshchrv fvavcfcaga qgctprrrvr vlieiaavcs
       61 stardankne dhqprstwrh fltpygcspg dqrldacdrr gallseiles ccgaaqetqp
      121 camlyhkell iprgteestg epehlfviga daghratvlv ahglcrrrcv gdpslllvle
      181 haasrfsria tnlaldvpga iaswvfktid daelavlqed laqdaahvic psadrvgrtp
      241 palasramht ikgaeevaml pqdlaqdtag vflpraallk rlvplrrlps ltpdktrknl
      301 ctsgwlparl plvsqaf
//
LOCUS       XP_077524056             131 aa            linear   INV 25-NOV-2025
DEFINITION  beta-scruin-like [Amblyomma americanum].
ACCESSION   XP_077524056
VERSION     XP_077524056.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667930.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..131
                     /product="beta-scruin-like"
                     /calculated_mol_wt=14593
     Region          1..>127
                     /region_name="NanM"
                     /note="N-acetylneuraminic acid mutarotase [Cell
                     wall/membrane/envelope biogenesis]; COG3055"
                     /db_xref="CDD:442289"
     Region          19..67
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     CDS             1..131
                     /gene="LOC144135186"
                     /coded_by="XM_077667930.1:13..408"
                     /db_xref="GeneID:144135186"
ORIGIN      
        1 mydlnakqws fihpmpmalm gmaaatidrr vyivggithg ldgdtlkdsv yafdlskhtw
       61 telpammekr afcsavpvqp dlwlvgrvve lkpeakcttr vdvlrlasst lerrcdmpsp
      121 rhfvclakig e
//
LOCUS       XP_077524065             485 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135196 [Amblyomma americanum].
ACCESSION   XP_077524065
VERSION     XP_077524065.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667939.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..485
                     /product="uncharacterized protein LOC144135196"
                     /calculated_mol_wt=48411
     CDS             1..485
                     /gene="LOC144135196"
                     /coded_by="XM_077667939.1:25..1482"
                     /db_xref="GeneID:144135196"
ORIGIN      
        1 mpceplprgl agaaaavmpg dcfdwplfrw iwpttkttph nrdravtvtq karlhretci
       61 papkgysdrn asgffgaaqy rpssdpaapp swrqrkplhs rhpalpasma ltappalpqa
      121 vptlqaasal lappahpasl aipappahpa cpalhpaltl papptiktas pypaapalpa
      181 pqavttaspf pvapaftaaa alpapadlla ppvlrvpsra lqtrqairal lallhasvps
      241 llsalqeari lqlartlpas alaarlafsa tpvlttasaf paalalpvpk aipvpqalpv
      301 pppvpalpvl latsahssrp alqaaltlpe dcalpasppl sapsalpvaa alpappvlla
      361 ppvfpvplsp rdtpghpdal gpprglgpla tlgpsggpdy pggpnslggl gnpgepgpps
      421 tpgppgtpvp psglgprftp gppggtdsps grgfrstpgl padlvsfgap gphdgpgpqg
      481 tpgtp
//
LOCUS       XP_077524087             336 aa            linear   INV 25-NOV-2025
DEFINITION  beta-scruin-like [Amblyomma americanum].
ACCESSION   XP_077524087
VERSION     XP_077524087.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667961.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..336
                     /product="beta-scruin-like"
                     /calculated_mol_wt=36872
     CDS             1..336
                     /gene="LOC144135219"
                     /coded_by="XM_077667961.1:15..1025"
                     /db_xref="GeneID:144135219"
ORIGIN      
        1 mmekraffsa ldvqrdwwla ggvmqlrpkv kcttcidvlr lasstmerps vmpsprhsvc
       61 aektgyevyl fvgqdennrp lgdvimfdra rrsimqcepl pwilagsata vmpedgldwl
      121 rlrsiwpgkk atpnireraa tviqkayrny rrgerlqprl kntwpppars tetleshwda
      181 ngaeasgatq ylrqqtfpen mdpnlgmvgr mdgsflktnk tatappdvla ptthptpqvh
      241 pvllsspahp aslvpaalka spadtaplai kappappalp ahatlpappa lppppahpay
      301 aadqepsvlp appdsppppa rrrshrirrk pdrwgy
//
LOCUS       XP_077524095             354 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135229 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077524095
VERSION     XP_077524095.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667969.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..354
                     /product="uncharacterized protein LOC144135229 isoform X1"
                     /calculated_mol_wt=39534
     CDS             1..354
                     /gene="LOC144135229"
                     /coded_by="XM_077667969.1:142..1206"
                     /db_xref="GeneID:144135229"
ORIGIN      
        1 mtksalvfna allaaailvp glvqlarqgk svedyydeei yydekavdgs adfseditya
       61 qydlggtllt vlrrvlerrc rlrglhhmar rqrgpshlrp akvenrtvtp sasteknasq
      121 nettvtttkp gsrrihvakq qdvptsktvq rattttektv qlqaprsnvt gmcriarsea
      181 wrqvslysec leipdvksnd rrmgevrflr rfrlasqtte ilwhyllphi pedtrqtdcp
      241 gsnlsqttgt tqfcnrsfqs aketvrvldt gpfsgdplqe whmvcrlvqq ykycvselvs
      301 gchgiqttei drkateerga vdatycfinc asglaatalw avvygivslc rhgp
//
LOCUS       XP_077524100             353 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135229 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077524100
VERSION     XP_077524100.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667974.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..353
                     /product="uncharacterized protein LOC144135229 isoform X2"
                     /calculated_mol_wt=39447
     CDS             1..353
                     /gene="LOC144135229"
                     /coded_by="XM_077667974.1:142..1203"
                     /db_xref="GeneID:144135229"
ORIGIN      
        1 mtksalvfna allaaailvp glvqlarqgk svedyydeei yydekavdgs adfseditya
       61 qydlggtllt vlrrvlerrc rlrglhhmar rqrgpshlrp akvenrtvtp sasteknasq
      121 nettvtttkp gsrrihvakq qdvptsktvq rattttekiq lqaprsnvtg mcriarseaw
      181 rqvslysecl eipdvksndr rmgevrflrr frlasqttei lwhyllphip edtrqtdcpg
      241 snlsqttgtt qfcnrsfqsa ketvrvldtg pfsgdplqew hmvcrlvqqy kycvselvsg
      301 chgiqtteid rkateergav datycfinca sglaatalwa vvygivslcr hgp
//
LOCUS       XP_077524105             313 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135229 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077524105
VERSION     XP_077524105.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667979.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..313
                     /product="uncharacterized protein LOC144135229 isoform X3"
                     /calculated_mol_wt=34655
     CDS             1..313
                     /gene="LOC144135229"
                     /coded_by="XM_077667979.1:142..1083"
                     /db_xref="GeneID:144135229"
ORIGIN      
        1 mtksalvfna allaaailvp glvqlarqgk svedyydeei yydekavdgs adfseditya
       61 qydlgtvtps asteknasqn ettvtttkpg srrihvakqq dvptsktvqr attttektvq
      121 lqaprsnvtg mcriarseaw rqvslysecl eipdvksndr rmgevrflrr frlasqttei
      181 lwhyllphip edtrqtdcpg snlsqttgtt qfcnrsfqsa ketvrvldtg pfsgdplqew
      241 hmvcrlvqqy kycvselvsg chgiqtteid rkateergav datycfinca sglaatalwa
      301 vvygivslcr hgp
//
LOCUS       XP_077524115             210 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135255 [Amblyomma americanum].
ACCESSION   XP_077524115
VERSION     XP_077524115.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667989.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..210
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..210
                     /product="uncharacterized protein LOC144135255"
                     /calculated_mol_wt=24280
     CDS             1..210
                     /gene="LOC144135255"
                     /coded_by="XM_077667989.1:87..719"
                     /db_xref="GeneID:144135255"
ORIGIN      
        1 mrwrcallsv awlqgvacmk lwrgvadkgp aptgppmhaa neddeyyled tgekeyyyyd
       61 qdeyyeyfqd rkpiagfrnr rlerslkklr rtiqaaavqg cnetqlinvr nmcerkmtla
      121 kklrrrrprn kkvrsvqwrk vcrlvtdysd cisaiapsre cpdiafklkt dalrsvhteg
      181 fqvcrspsve pfaclvvaaa styaqrllqn
//
LOCUS       XP_077524121             505 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135268 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077524121
VERSION     XP_077524121.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667995.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..505
                     /product="uncharacterized protein LOC144135268 isoform X1"
                     /calculated_mol_wt=56187
     CDS             1..505
                     /gene="LOC144135268"
                     /coded_by="XM_077667995.1:223..1740"
                     /db_xref="GeneID:144135268"
ORIGIN      
        1 mrplyallla iavaasnatr slfeevkrld algvddktkt aaegvslpgs ehtdkpgand
       61 cdknkfregi nncenglkpl ekylsekknd dsckllkiyl sciedfqlfl scendpasqr
      121 emakatsiiv knrlnctels fltanltsqp ptqnaaaiis kstspptvts avtkvplvsk
      181 kcserklmrr mvdclksmkg nleplldnkk anqklicsqt enfkscvnia lahtdcnsde
      241 evldqtthfi vhvlyehrwa ckniqtsekk ceerslvlkl nqcvrllnrt vepiledkdn
      301 nklnicrasd tfllcvneav setacredse vmfhvshfad kvlrhhkwsc eqgaassnqp
      361 rmenlhqnmd aigsgnltai rsfgggipmv ltppercnfe avrtsvdtcq qsaydqikkk
      421 gisqpkhlcv elrifkdcsi eatrkngcat dknaetyvyd rvkdsfykyl tdcrplwsaa
      481 tplsaaasfw alalaglmvf aqgrh
//
LOCUS       XP_077524124             504 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135268 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077524124
VERSION     XP_077524124.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077667998.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..504
                     /product="uncharacterized protein LOC144135268 isoform X2"
                     /calculated_mol_wt=56189
     CDS             1..504
                     /gene="LOC144135268"
                     /coded_by="XM_077667998.1:223..1737"
                     /db_xref="GeneID:144135268"
ORIGIN      
        1 mrplyallla iavaasnatr slfeevkrld algvddktkt aaegvslrse htdkpgandc
       61 dknkfregin ncenglkple kylsekkndd sckllkiyls ciedfqlfls cendpasqre
      121 makatsiivk nrlnctelsf ltanltsqpp tqnaaaiisk stspptvtsa vtkvplvskk
      181 cserklmrrm vdclksmkgn leplldnkka nqklicsqte nfkscvnial ahtdcnsdee
      241 vldqtthfiv hvlyehrwac kniqtsekkc eerslvlkln qcvrllnrtv epiledkdnn
      301 klnicrasdt fllcvneavs etacredsev mfhvshfadk vlrhhkwsce qgaassnqpr
      361 menlhqnmda igsgnltair sfgggipmvl tppercnfea vrtsvdtcqq saydqikkkg
      421 isqpkhlcve lrifkdcsie atrkngcatd knaetyvydr vkdsfykylt dcrplwsaat
      481 plsaaasfwa lalaglmvfa qgrh
//
LOCUS       XP_077524129             414 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135268 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077524129
VERSION     XP_077524129.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668003.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..414
                     /product="uncharacterized protein LOC144135268 isoform X3"
                     /calculated_mol_wt=45643
     CDS             1..414
                     /gene="LOC144135268"
                     /coded_by="XM_077668003.1:223..1467"
                     /db_xref="GeneID:144135268"
ORIGIN      
        1 mrplyallla iavaasnatr slfeevkrld algvddktkt aaegvslpgs ehtdkpgand
       61 cdknkfregi nncenglkpl ekylsekknd dsckllkiyl sciedfqlfl scendpasqr
      121 emakatsiiv knrlnctels fltanltsqp ptqnaaaiis kstspptvts avtkasekkc
      181 eerslvlkln qcvrllnrtv epiledkdnn klnicrasdt fllcvneavs etacredsev
      241 mfhvshfadk vlrhhkwsce qgaassnqpr menlhqnmda igsgnltair sfgggipmvl
      301 tppercnfea vrtsvdtcqq saydqikkkg isqpkhlcve lrifkdcsie atrkngcatd
      361 knaetyvydr vkdsfykylt dcrplwsaat plsaaasfwa lalaglmvfa qgrh
//
LOCUS       XP_077524137             148 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135298 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077524137
VERSION     XP_077524137.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668011.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="uncharacterized protein LOC144135298 isoform X1"
                     /calculated_mol_wt=16759
     CDS             1..148
                     /gene="LOC144135298"
                     /coded_by="XM_077668011.1:419..865"
                     /db_xref="GeneID:144135298"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedrrr rhlnrrltat mkrlsgvhvl
       61 nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar pgevyvvhrc
      121 rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077524143              98 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135298 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077524143
VERSION     XP_077524143.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668017.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..98
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..98
                     /product="uncharacterized protein LOC144135298 isoform X2"
                     /calculated_mol_wt=10604
     CDS             1..98
                     /gene="LOC144135298"
                     /coded_by="XM_077668017.1:498..794"
                     /db_xref="GeneID:144135298"
ORIGIN      
        1 mkrlsgvhvl nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar
       61 pgevyvvhrc rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077524168             175 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135360 [Amblyomma americanum].
ACCESSION   XP_077524168
VERSION     XP_077524168.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668042.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..175
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..175
                     /product="uncharacterized protein LOC144135360"
                     /calculated_mol_wt=18923
     CDS             1..175
                     /gene="LOC144135360"
                     /coded_by="XM_077668042.1:21..548"
                     /db_xref="GeneID:144135360"
ORIGIN      
        1 mvigsspsip rpkadtryss trltwsthgw rtlfhnvrsg aptgppvpvg vlrrwqaach
       61 rhrpgprgga ahaedfrgqa pntpaaanrr ntssspngqc delgdgcsrp psaaskvlpm
      121 amhepypgrq wrsvtfddak rrviatdqda evdtlnlksi vgrpptlrps rtdcc
//
LOCUS       XP_077524171             175 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135368 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077524171
VERSION     XP_077524171.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668045.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..175
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..175
                     /product="uncharacterized protein LOC144135368 isoform X1"
                     /calculated_mol_wt=18939
     CDS             1..175
                     /gene="LOC144135368"
                     /coded_by="XM_077668045.1:78..605"
                     /db_xref="GeneID:144135368"
ORIGIN      
        1 mvigsspsip rpkadtryss trltwsthgw rtlfhnvrsg aptgppvpvg vlrrwqaach
       61 rhrpgsrggp ahaedfrgqa pntpaaanrr ntssspngqc delgdgcsrp psaaskvlpm
      121 amhepypgrq wrsvtfddak rrviatdqda evdtlnlksi vgrpptlrps rtdcc
//
LOCUS       XP_077524178             140 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135368 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077524178
VERSION     XP_077524178.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668052.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..140
                     /product="uncharacterized protein LOC144135368 isoform X2"
                     /calculated_mol_wt=14958
     CDS             1..140
                     /gene="LOC144135368"
                     /coded_by="XM_077668052.1:75..497"
                     /db_xref="GeneID:144135368"
ORIGIN      
        1 mageraptgp pvpvgvlrrw qaachrhrpg srggpahaed frgqapntpa aanrrntsss
       61 pngqcdelgd gcsrppsaas kvlpmamhep ypgrqwrsvt fddakrrvia tdqdaevdtl
      121 nlksivgrpp tlrpsrtdcc
//
LOCUS       XP_077524202             233 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135410 [Amblyomma americanum].
ACCESSION   XP_077524202
VERSION     XP_077524202.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668076.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..233
                     /product="uncharacterized protein LOC144135410"
                     /calculated_mol_wt=26032
     Region          <54..180
                     /region_name="TesA"
                     /note="Lysophospholipase L1 or related esterase. Includes
                     spore coat protein LipC/YcsK [Cell cycle control, cell
                     division, chromosome partitioning, Lipid transport and
                     metabolism]; COG2755"
                     /db_xref="CDD:442045"
     CDS             1..233
                     /gene="LOC144135410"
                     /coded_by="XM_077668076.1:26..727"
                     /db_xref="GeneID:144135410"
ORIGIN      
        1 maflrgvlvg dsqlkflcss rlrlspvvrt ctfsfsghda islaeiiiet cfrradfvvl
       61 yvggndldrg ddpreiashi kdlvlllqen vasvvlvfkv lprhfdeprk aqfedrrrrq
      121 lnrrlsatlk rlsgvhilnp ehrfldasgk pmlslfaadr yhvardrgid qmskiivaal
      181 vkiygpgias asrarpgevy vvnrcrrcga kghktdhcwa ycsprrhaaa grg
//
LOCUS       XP_077524213             192 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135420 [Amblyomma americanum].
ACCESSION   XP_077524213
VERSION     XP_077524213.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668087.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..192
                     /product="uncharacterized protein LOC144135420"
                     /calculated_mol_wt=21536
     CDS             1..192
                     /gene="LOC144135420"
                     /coded_by="XM_077668087.1:421..999"
                     /db_xref="GeneID:144135420"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedrrr rhlnrrltat mkrlsgvhvl
       61 nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar pgevyvvhrc
      121 rrcgakghkt dhcgptahra atplqvavev llqtalfrat slfpgrsras rfvpafplcr
      181 nrrqtsksrg vs
//
LOCUS       XP_077524227             148 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135433 [Amblyomma americanum].
ACCESSION   XP_077524227
VERSION     XP_077524227.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668101.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="uncharacterized protein LOC144135433"
                     /calculated_mol_wt=16759
     CDS             1..148
                     /gene="LOC144135433"
                     /coded_by="XM_077668101.1:270..716"
                     /db_xref="GeneID:144135433"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedrrr rhlnrrltat mkrlsgvhvl
       61 nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar pgevyvvhrc
      121 rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077524260             445 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135474 [Amblyomma americanum].
ACCESSION   XP_077524260
VERSION     XP_077524260.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668134.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..445
                     /product="uncharacterized protein LOC144135474"
                     /calculated_mol_wt=47796
     Region          13..68
                     /region_name="MIT"
                     /note="domain contained within Microtubule Interacting and
                     Trafficking molecules. The MIT domain is found in sorting
                     nexins, the nuclear thiol protease PalBH, the AAA protein
                     spastin and archaebacterial proteins with similar domain
                     architecture, vacuolar...; cl00299"
                     /db_xref="CDD:469712"
     Region          114..>161
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Region          145..277
                     /region_name="AAA"
                     /note="ATPase family associated with various cellular
                     activities (AAA); pfam00004"
                     /db_xref="CDD:459627"
     Region          <242..317
                     /region_name="ycf46"
                     /note="Ycf46; Provisional; CHL00195"
                     /db_xref="CDD:177094"
     CDS             1..445
                     /gene="LOC144135474"
                     /coded_by="XM_077668134.1:87..1424"
                     /db_xref="GeneID:144135474"
ORIGIN      
        1 mvipvtlind ikqavdlskq ahaadnkeqy anairlyrka mesyvnaall ignscapfie
       61 raqqlkaffg sdanedvvve gmpemkkihq iavlsqvlpp wrtaagtvts gkkinfcddi
      121 qgidpaklll tqiladpkak aastillygp hgtgksllvk slyvkypekf ifivdldffv
      181 igglthdpal mtrmlvrnym khntavlile gldrlylpea peakkpvvda akaellaymk
      241 elvekkeyke vviatasqpw liaedmrstf eakinvpipe seearkaiik velfklrvps
      301 ycagdigsla kntnrfsryq llriircala rnfhkieaie ikenaerlsh mtkadmdkva
      361 plftadlsee depkhqefia annpapaasp aaaaspnava sppaaaassa aaatspaaas
      421 asppaaaaas ppaaaspapa appan
//
LOCUS       XP_077524268             365 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135487 [Amblyomma americanum].
ACCESSION   XP_077524268
VERSION     XP_077524268.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668142.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..365
                     /product="uncharacterized protein LOC144135487"
                     /calculated_mol_wt=38446
     CDS             1..365
                     /gene="LOC144135487"
                     /coded_by="XM_077668142.1:10..1107"
                     /db_xref="GeneID:144135487"
ORIGIN      
        1 mssserpsss sgkpraptpk gsvqslgckv rgpwwtdgga gdldyafles pnptaplrqg
       61 nepgekmvtv yaqsripeva dpqhqetase kpssststad lenapresfl pkkrkeeadg
      121 qefvglkpad tlvangaeay gvdqrsprqi nrhppralch msspspgese dttafdflrl
      181 igrnitcrrr tgitscdldl sslespspaa avpeisdlas aqmvtratgq eksshevgta
      241 sqkapdeppt lasrtdsekg shasfarske nepaavtglg gmgpsthpaa tiepvpgvdq
      301 gnsrptkrqg aktsrrrgar retsedgtts svasstvdge svarstsgrp lrsaaigkny
      361 resdi
//
LOCUS       XP_077524277             132 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135499 [Amblyomma americanum].
ACCESSION   XP_077524277
VERSION     XP_077524277.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668151.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..132
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..132
                     /product="uncharacterized protein LOC144135499"
                     /calculated_mol_wt=14058
     CDS             1..132
                     /gene="LOC144135499"
                     /coded_by="XM_077668151.1:58..456"
                     /db_xref="GeneID:144135499"
ORIGIN      
        1 mgtrvynpfd tdgshysnsg plqctptvga lleatqsepq aeadefdfpw cratknsqae
       61 sgeaaevaev apddqeaysn qdlqeqqcsd gdhnapilat tcevilpaaq pagyqrrkaa
      121 papkesvclr qs
//
LOCUS       XP_077524303             237 aa            linear   INV 25-NOV-2025
DEFINITION  U-scoloptoxin(11)-Ssd3a-like [Amblyomma americanum].
ACCESSION   XP_077524303
VERSION     XP_077524303.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668177.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..237
                     /product="U-scoloptoxin(11)-Ssd3a-like"
                     /calculated_mol_wt=26087
     CDS             1..237
                     /gene="LOC144135530"
                     /coded_by="XM_077668177.1:55..768"
                     /db_xref="GeneID:144135530"
ORIGIN      
        1 masgarrrcl vsaalcfcsl gaalalvvyg ngatwerelp pcasersacs lvhrrywlsp
       61 levrlcrcpr kepcpegfdt apggsalnvt srtqlkvcsg whapvcnasq vslvtkvvrk
      121 lgpgaqandt rqthadvvcr csvqdaeiyy rrahgnstas styyeqrqyf tcesipdckp
      181 eepcghvtrd fyaiyrtcnc ppnnlctvpm nfsdrrlvnv rkalyegaay valcqpy
//
LOCUS       XP_077524319             630 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135548 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077524319
VERSION     XP_077524319.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668193.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..630
                     /product="uncharacterized protein LOC144135548 isoform X1"
                     /calculated_mol_wt=67793
     Region          <14..378
                     /region_name="PRK12323"
                     /note="DNA polymerase III subunit gamma/tau; cl46901"
                     /db_xref="CDD:481241"
     Region          317..585
                     /region_name="S_TKc"
                     /note="Serine/Threonine protein kinases, catalytic domain;
                     smart00220"
                     /db_xref="CDD:214567"
     Site            order(320..324,328,341,343,376,392..393,395,399,401,438,
                     440,442..443,445,455..456,459,470..474,501,507,512)
                     /site_type="active"
                     /db_xref="CDD:270693"
     Site            order(320..324,328,341,343,376,393..395,399,438,440,
                     442..443,445,455..456)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270693"
     Site            order(324,399,401,438,440,442,459,470..474,501,507,512)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270693"
     Site            order(455..462,470..474)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270693"
     CDS             1..630
                     /gene="LOC144135548"
                     /coded_by="XM_077668193.1:134..2026"
                     /db_xref="GeneID:144135548"
ORIGIN      
        1 mlicadvpip tlhalapgll ftchvphrss kvrpgtpngs lrhgaavpps ssrpnavagq
       61 prtaaeraps rpgtssrape rpaqaaasqv sglsvvttsq dsaprtprsd aeqllqapaa
      121 qpgattpfrd tteadgertp kqptscvvsp sgapvspgsk agspmrrnsp pghaassahs
      181 pvqptspped paavrggqdp ddsnslkwms tkepgakvkr rddeessagp shvhlqsaaa
      241 spvapktrkq rtsqgvaett trkssggsrt elcgifsgll grcrlksqis lgdvddvtfw
      301 vnpdeasdwr lsslqqlqvl gkgsfgvvyl vrhkatkela alkciekhhr eqakearrve
      361 aermawqavs hhpfvatlka yyettkawcf vmeyvprgtl sqiirrsgpl peskaklvsa
      421 qlahalnfvh tagylhrdis ssnvllderg narlidfglc qlglearnrc gslaymapev
      481 lrrehygvga dwwsfgivly tmligktpls qyaaeqnini qttrrelrys malaaplrfp
      541 rsltdeayni lqeilredps mrlgcrtsgg pgnsgfqemm qhkffstvdw ramigdvrqv
      601 grsgsrnprd eaapsggkas sagassrqlk
//
LOCUS       XP_077524325             628 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135548 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077524325
VERSION     XP_077524325.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668199.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..628
                     /product="uncharacterized protein LOC144135548 isoform X2"
                     /calculated_mol_wt=67578
     Region          <18..317
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          315..583
                     /region_name="S_TKc"
                     /note="Serine/Threonine protein kinases, catalytic domain;
                     smart00220"
                     /db_xref="CDD:214567"
     Site            order(318..322,326,339,341,374,390..391,393,397,399,436,
                     438,440..441,443,453..454,457,468..472,499,505,510)
                     /site_type="active"
                     /db_xref="CDD:270693"
     Site            order(318..322,326,339,341,374,391..393,397,436,438,
                     440..441,443,453..454)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270693"
     Site            order(322,397,399,436,438,440,457,468..472,499,505,510)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270693"
     Site            order(453..460,468..472)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270693"
     CDS             1..628
                     /gene="LOC144135548"
                     /coded_by="XM_077668199.1:134..2020"
                     /db_xref="GeneID:144135548"
ORIGIN      
        1 mlicadvpip tlhalapgll ftchvphrss kvrpgtpngs lrhgaavpps ssrpnavagq
       61 prtaaeraps rpgtssrape rpaqaaavsg lsvvttsqds aprtprsdae qllqapaaqp
      121 gattpfrdtt eadgertpkq ptscvvspsg apvspgskag spmrrnsppg haassahspv
      181 qptsppedpa avrggqdpdd snslkwmstk epgakvkrrd deessagpsh vhlqsaaasp
      241 vapktrkqrt sqgvaetttr kssggsrtel cgifsgllgr crlksqislg dvddvtfwvn
      301 pdeasdwrls slqqlqvlgk gsfgvvylvr hkatkelaal kciekhhreq akearrveae
      361 rmawqavshh pfvatlkayy ettkawcfvm eyvprgtlsq iirrsgplpe skaklvsaql
      421 ahalnfvhta gylhrdisss nvlldergna rlidfglcql glearnrcgs laymapevlr
      481 rehygvgadw wsfgivlytm ligktplsqy aaeqniniqt trrelrysma laaplrfprs
      541 ltdeaynilq eilredpsmr lgcrtsggpg nsgfqemmqh kffstvdwra migdvrqvgr
      601 sgsrnprdea apsggkassa gassrqlk
//
LOCUS       XP_077524326             574 aa            linear   INV 25-NOV-2025
DEFINITION  protein kinase C-like 3 isoform X3 [Amblyomma americanum].
ACCESSION   XP_077524326
VERSION     XP_077524326.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668200.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..574
                     /product="protein kinase C-like 3 isoform X3"
                     /calculated_mol_wt=62231
     Region          261..529
                     /region_name="S_TKc"
                     /note="Serine/Threonine protein kinases, catalytic domain;
                     smart00220"
                     /db_xref="CDD:214567"
     Site            order(264..268,272,285,287,320,336..337,339,343,345,382,
                     384,386..387,389,399..400,403,414..418,445,451,456)
                     /site_type="active"
                     /db_xref="CDD:270693"
     Site            order(264..268,272,285,287,320,337..339,343,382,384,
                     386..387,389,399..400)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270693"
     Site            order(268,343,345,382,384,386,403,414..418,445,451,456)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270693"
     Site            order(399..406,414..418)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270693"
     CDS             1..574
                     /gene="LOC144135548"
                     /coded_by="XM_077668200.1:134..1858"
                     /db_xref="GeneID:144135548"
ORIGIN      
        1 mlicadvpip tlhalapgll ftchvphrss ksqvsglsvv ttsqdsaprt prsdaeqllq
       61 apaaqpgatt pfrdtteadg ertpkqptsc vvspsgapvs pgskagspmr rnsppghaas
      121 sahspvqpts ppedpaavrg gqdpddsnsl kwmstkepga kvkrrddees sagpshvhlq
      181 saaaspvapk trkqrtsqgv aetttrkssg gsrtelcgif sgllgrcrlk sqislgdvdd
      241 vtfwvnpdea sdwrlsslqq lqvlgkgsfg vvylvrhkat kelaalkcie khhreqakea
      301 rrveaermaw qavshhpfva tlkayyettk awcfvmeyvp rgtlsqiirr sgplpeskak
      361 lvsaqlahal nfvhtagylh rdisssnvll dergnarlid fglcqlglea rnrcgslaym
      421 apevlrrehy gvgadwwsfg ivlytmligk tplsqyaaeq niniqttrre lrysmalaap
      481 lrfprsltde aynilqeilr edpsmrlgcr tsggpgnsgf qemmqhkffs tvdwramigd
      541 vrqvgrsgsr nprdeaapsg gkassagass rqlk
//
LOCUS       XP_077524327             572 aa            linear   INV 25-NOV-2025
DEFINITION  protein kinase C-like 3 isoform X4 [Amblyomma americanum].
ACCESSION   XP_077524327
VERSION     XP_077524327.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668201.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..572
                     /product="protein kinase C-like 3 isoform X4"
                     /calculated_mol_wt=62015
     Region          259..527
                     /region_name="S_TKc"
                     /note="Serine/Threonine protein kinases, catalytic domain;
                     smart00220"
                     /db_xref="CDD:214567"
     Site            order(262..266,270,283,285,318,334..335,337,341,343,380,
                     382,384..385,387,397..398,401,412..416,443,449,454)
                     /site_type="active"
                     /db_xref="CDD:270693"
     Site            order(262..266,270,283,285,318,335..337,341,380,382,
                     384..385,387,397..398)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270693"
     Site            order(266,341,343,380,382,384,401,412..416,443,449,454)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270693"
     Site            order(397..404,412..416)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270693"
     CDS             1..572
                     /gene="LOC144135548"
                     /coded_by="XM_077668201.1:134..1852"
                     /db_xref="GeneID:144135548"
ORIGIN      
        1 mlicadvpip tlhalapgll ftchvphrss kvsglsvvtt sqdsaprtpr sdaeqllqap
       61 aaqpgattpf rdtteadger tpkqptscvv spsgapvspg skagspmrrn sppghaassa
      121 hspvqptspp edpaavrggq dpddsnslkw mstkepgakv krrddeessa gpshvhlqsa
      181 aaspvapktr kqrtsqgvae tttrkssggs rtelcgifsg llgrcrlksq islgdvddvt
      241 fwvnpdeasd wrlsslqqlq vlgkgsfgvv ylvrhkatke laalkciekh hreqakearr
      301 veaermawqa vshhpfvatl kayyettkaw cfvmeyvprg tlsqiirrsg plpeskaklv
      361 saqlahalnf vhtagylhrd isssnvllde rgnarlidfg lcqlglearn rcgslaymap
      421 evlrrehygv gadwwsfgiv lytmligktp lsqyaaeqni niqttrrelr ysmalaaplr
      481 fprsltdeay nilqeilred psmrlgcrts ggpgnsgfqe mmqhkffstv dwramigdvr
      541 qvgrsgsrnp rdeaapsggk assagassrq lk
//
LOCUS       XP_077524328             546 aa            linear   INV 25-NOV-2025
DEFINITION  serine/threonine-protein kinase Sgk1-like isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077524328
VERSION     XP_077524328.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668202.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..546
                     /product="serine/threonine-protein kinase Sgk1-like
                     isoform X5"
                     /calculated_mol_wt=58828
     Region          <16..>197
                     /region_name="PRK07003"
                     /note="DNA polymerase III subunit gamma/tau"
                     /db_xref="CDD:235906"
     Region          233..501
                     /region_name="S_TKc"
                     /note="Serine/Threonine protein kinases, catalytic domain;
                     smart00220"
                     /db_xref="CDD:214567"
     Site            order(236..240,244,257,259,292,308..309,311,315,317,354,
                     356,358..359,361,371..372,375,386..390,417,423,428)
                     /site_type="active"
                     /db_xref="CDD:270693"
     Site            order(236..240,244,257,259,292,309..311,315,354,356,
                     358..359,361,371..372)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270693"
     Site            order(240,315,317,354,356,358,375,386..390,417,423,428)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270693"
     Site            order(371..378,386..390)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270693"
     CDS             1..546
                     /gene="LOC144135548"
                     /coded_by="XM_077668202.1:134..1774"
                     /db_xref="GeneID:144135548"
ORIGIN      
        1 mlicadvpip tlhalapgll ftchvphrss kvrpgtpngs lrhgaavpps ssrpnavagq
       61 prtaaeraps rpgtssrape rpaqaaasqv sglsvvttsq dsaprtprsd aeqllqapaa
      121 qpgattpfrd tteadgertp kqptscvvsp sgapvspgsk agspmrrnsp pghaassahs
      181 pvqptspped paavrggqdp ddsqislgdv ddvtfwvnpd easdwrlssl qqlqvlgkgs
      241 fgvvylvrhk atkelaalkc iekhhreqak earrveaerm awqavshhpf vatlkayyet
      301 tkawcfvmey vprgtlsqii rrsgplpesk aklvsaqlah alnfvhtagy lhrdisssnv
      361 lldergnarl idfglcqlgl earnrcgsla ymapevlrre hygvgadwws fgivlytmli
      421 gktplsqyaa eqniniqttr relrysmala aplrfprslt deaynilqei lredpsmrlg
      481 crtsggpgns gfqemmqhkf fstvdwrami gdvrqvgrsg srnprdeaap sggkassaga
      541 ssrqlk
//
LOCUS       XP_077524329             479 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135548 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077524329
VERSION     XP_077524329.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668203.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..479
                     /product="uncharacterized protein LOC144135548 isoform X6"
                     /calculated_mol_wt=51255
     Region          <16..>197
                     /region_name="PRK07003"
                     /note="DNA polymerase III subunit gamma/tau"
                     /db_xref="CDD:235906"
     Region          199..434
                     /region_name="S_TKc"
                     /note="Serine/Threonine protein kinases, catalytic domain;
                     smart00220"
                     /db_xref="CDD:214567"
     CDS             1..479
                     /gene="LOC144135548"
                     /coded_by="XM_077668203.1:134..1573"
                     /db_xref="GeneID:144135548"
ORIGIN      
        1 mlicadvpip tlhalapgll ftchvphrss kvrpgtpngs lrhgaavpps ssrpnavagq
       61 prtaaeraps rpgtssrape rpaqaaasqv sglsvvttsq dsaprtprsd aeqllqapaa
      121 qpgattpfrd tteadgertp kqptscvvsp sgapvspgsk agspmrrnsp pghaassahs
      181 pvqptspped paavrggqdp ddsearrvea ermawqavsh hpfvatlkay yettkawcfv
      241 meyvprgtls qiirrsgplp eskaklvsaq lahalnfvht agylhrdiss snvlldergn
      301 arlidfglcq lglearnrcg slaymapevl rrehygvgad wwsfgivlyt mligktplsq
      361 yaaeqniniq ttrrelrysm alaaplrfpr sltdeaynil qeilredpsm rlgcrtsggp
      421 gnsgfqemmq hkffstvdwr amigdvrqvg rsgsrnprde aapsggkass agassrqlk
//
LOCUS       XP_077524330             694 aa            linear   INV 25-NOV-2025
DEFINITION  hemocyte protein-glutamine gamma-glutamyltransferase-like
            [Amblyomma americanum].
ACCESSION   XP_077524330
VERSION     XP_077524330.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668204.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..694
                     /product="hemocyte protein-glutamine
                     gamma-glutamyltransferase-like"
                     /calculated_mol_wt=76042
     Region          35..152
                     /region_name="Transglut_N"
                     /note="Transglutaminase family; pfam00868"
                     /db_xref="CDD:459971"
     Region          295..383
                     /region_name="TGc"
                     /note="Transglutaminase/protease-like homologues;
                     smart00460"
                     /db_xref="CDD:214673"
     CDS             1..694
                     /gene="LOC144135608"
                     /coded_by="XM_077668204.1:350..2434"
                     /db_xref="GeneID:144135608"
ORIGIN      
        1 mprvprlaal lagflcwlww savvsgqdgg givsslnlfm eenaathhte pfkilrgntp
       61 slvvrrgfaf rlavtssast ltiilslgen pqagdgslvq lavpadphta sshpapgqmq
      121 wtlalkqqeg parllevgip vtagvgrwll tigadgqhvr aresiyilfn pwhngdlvfm
      181 endhqrsfyv mqdrgiifhg qvygpvakew yygqfekssl psaefllkis elpasergnp
      241 iavsrklasg vnskdnngvv qgrwnppysd gvhpfawsst sailnqymem ggntvlygqc
      301 fvfaavlntl lralgipcrv vtnfpsvhtd nggqlldirf pvnggrpqfa gsvwnyhvwn
      361 egwmqrpdlp ngyggwqvld gtpqglsiqs rlfevgpfpv ravledkidm pydgnvaraa
      421 vkatyryfaa dpsdpsgwrl fkvlenqcgr lvateaigtg qmqdithtyk arpmmgmarh
      481 akeepvgvkv rhekkvpvgq pigiscavsn nqhkpchasv tvivtsityn nefpvtissv
      541 aynvtlpagq ektyhitvrp qdyvktlrge cmisieatvt ygkeiaysqs ilivqlpkln
      601 vdvapigpsg evhynvsmkn plaipltncq lslelpgstr flrpvsvgsv nayglffksg
      661 smfktseyth efnaafhcre mpvmsgyrsl qvps
//
LOCUS       XP_077524331             714 aa            linear   INV 25-NOV-2025
DEFINITION  hemocyte protein-glutamine gamma-glutamyltransferase-like
            [Amblyomma americanum].
ACCESSION   XP_077524331
VERSION     XP_077524331.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668205.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..714
                     /product="hemocyte protein-glutamine
                     gamma-glutamyltransferase-like"
                     /calculated_mol_wt=79176
     Region          53..174
                     /region_name="Transglut_N"
                     /note="Transglutaminase family; pfam00868"
                     /db_xref="CDD:459971"
     Region          317..406
                     /region_name="TGc"
                     /note="Transglutaminase/protease-like homologues;
                     smart00460"
                     /db_xref="CDD:214673"
     CDS             1..714
                     /gene="LOC144135625"
                     /coded_by="XM_077668205.1:81..2225"
                     /db_xref="GeneID:144135625"
ORIGIN      
        1 maaagdiepf lefvktkvhm pedletmaav lefmresttl mghtagplei veinmheksn
       61 aktnntesyk tvhgefpcnv lrrgnvfkvg vrfnqffdlg rvplraifki gtrpsegdgt
      121 lvtltapdav dkegrahetv drwtmsvsfh nndimllkfr spakapvgrw slsfslgdgs
      181 lhpakapiyi icnpwlpedp vymkddaqrq fytmketgay yygteknfgq rewnygqfek
      241 vtlatiefll kavdadgaqr snsvnlvral caainvqddq glltgrwsdd ysggtnpgvw
      301 sstmpilnky menggnsvky gqcfvfasal vtvlralgip srsvsnfpsa hvegddmsld
      361 yyfdeknkpi egadmewnfh vwteawmarp dlptgygewq avdstpqvvs egmyqvgpyp
      421 vkairenrtd lkhdgkylsa svkavykyhk kdpshpkgwk lvyqksnmcg rliltekfnp
      481 rggrqqpddi thtykgaaaq prgatmdliq vrlehekqva agqparvlca ltnndledhr
      541 vgvvltaysa pysnvspsvl vsaqrdfclq ptasdtlell ispeayvskl cpegiillea
      601 iatygddfta arsnlaiqmp qldiqivrre apigsltykl tmknplnval sgceltvelp
      661 gstaflekia lksvpprgtf ekegvlfvts pdtqrivatf mskqlpivna akdf
//
LOCUS       XP_077524332             799 aa            linear   INV 25-NOV-2025
DEFINITION  putative E3 ubiquitin-protein ligase HECTD2 [Amblyomma americanum].
ACCESSION   XP_077524332
VERSION     XP_077524332.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668206.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..799
                     /product="putative E3 ubiquitin-protein ligase HECTD2"
                     /calculated_mol_wt=90219
     Region          439..797
                     /region_name="HECTc"
                     /note="HECT domain; C-terminal catalytic domain of a
                     subclass of Ubiquitin-protein ligase (E3). It binds
                     specific ubiquitin-conjugating enzymes (E2), accepts
                     ubiquitin from E2, transfers ubiquitin to substrate lysine
                     side chains, and transfers additional...; cd00078"
                     /db_xref="CDD:238033"
     Site            order(445,474,485,542,732,761..762,765..769,789,796)
                     /site_type="active"
                     /note="catalytic cleft [active]"
                     /db_xref="CDD:238033"
     Site            order(585,588..589,591..592,595,603,605,609..610,612,617,
                     622,639,643)
                     /site_type="active"
                     /note="E2 interaction site [active]"
                     /db_xref="CDD:238033"
     CDS             1..799
                     /gene="LOC144135641"
                     /coded_by="XM_077668206.1:1..2400"
                     /db_xref="GeneID:144135641"
ORIGIN      
        1 mdaygaggre savsltcpac risavssrar amcpfcgsfy nrdansntsl rrrtapgdsl
       61 phidsqrslv rvtgfgplit dslgaihayi tglilrppsp galysslppv qqdaerrlct
      121 qsagsppepa kdtgsvygre qllpeyplrr rdvreirelv eegrasscys kvqefyeqtf
      181 sslvelnalf kenpnqdnak lddpeiktdl lhavydmlee lpsyiskavl kgiinsllat
      241 dirlkskddt ravymllqnp ifssqasytv fahllrkvvs lkssdhqlli hwfsnssper
      301 lralvkrllq fitirefppa nghklpsisk srwwipcatr vlalinaann kknpniltyt
      361 efynsaldhi dlmseyyrwq spgkhtkfsy cqypfilsiv akksilqrds eqqmilnarr
      421 sllnralhhq apdidvffln lnvrrshlvs dslneiarkq sdlkkklkvt fvgepgldmg
      481 gltkewflll irqifspdyg mfvyhnkarc ywfsttqlgn lreynligvl mglavynsii
      541 ldlhfptacy kkllnppvvp hnienayvgi ckfttddlae vmpdvavgln ellayegnve
      601 edfcmnfqvs veefgevkth tlkyggedip vtsenraeyv dlyvdlvlnk aiaqpfkafy
      661 lgfhsvcasn alimlrpeev emlvcgcpkl dldelrkvtv ydsfeedepi irdfwdilks
      721 fnpdvqkqfl rfatgsdrvp vggmgemafk isaqnsntdm lpvshtcfnq lvlpkyknkd
      781 ilrekltiai snaegfgle
//
LOCUS       XP_077524334             180 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135745 [Amblyomma americanum].
ACCESSION   XP_077524334
VERSION     XP_077524334.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668208.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..180
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..180
                     /product="uncharacterized protein LOC144135745"
                     /calculated_mol_wt=19517
     CDS             1..180
                     /gene="LOC144135745"
                     /coded_by="XM_077668208.1:351..893"
                     /db_xref="GeneID:144135745"
ORIGIN      
        1 mapspwklsl apcvqllngg rldldgtrpr haitatflvd atenaaqils qkhrsdllmp
       61 krlffakhhr cedppavdkd hvgkrkhhvg lpvnaghngi vdglqhahll ikpegsgcay
      121 vtrpcihlnt tqagmglest antssslfag spwwyagrsa igkatswswf stqlardfpe
//
LOCUS       XP_077524335             179 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135761 [Amblyomma americanum].
ACCESSION   XP_077524335
VERSION     XP_077524335.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668209.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..179
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..179
                     /product="uncharacterized protein LOC144135761"
                     /calculated_mol_wt=21037
     Region          3..>62
                     /region_name="THAP"
                     /note="The THAP domain is a putative DNA-binding domain
                     (DBD) and probably also binds a zinc ion; smart00980"
                     /db_xref="CDD:214951"
     CDS             1..179
                     /gene="LOC144135761"
                     /coded_by="XM_077668209.1:1..540"
                     /db_xref="GeneID:144135761"
ORIGIN      
        1 mgrccvpncr gnydkgpkvr vfsfpkddrr akweravrrd dvdirslrdp kvcelhfkae
       61 ylrttttytd prtgrtvevc rycleelnfd yvflgkfqtd sleerfgqnr rlsgtnyhis
      121 iqqvfesekk lrlqdslvlp dmqemqkpcs etldgaqlte eygikvpdhd irirnpfcr
//
LOCUS       XP_077524336             175 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135777 [Amblyomma americanum].
ACCESSION   XP_077524336
VERSION     XP_077524336.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668210.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..175
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..175
                     /product="uncharacterized protein LOC144135777"
                     /calculated_mol_wt=17839
     CDS             1..175
                     /gene="LOC144135777"
                     /coded_by="XM_077668210.1:246..773"
                     /db_xref="GeneID:144135777"
ORIGIN      
        1 mhpgrrgvds etafviaiiv vfivigvfvf iyaccvteek lksatkkkas qkkkdagpaa
       61 ppapapagpa apaaapvaap adsmakpsta aapspptngg gdtrnteivl tvqdtvaqsl
      121 pvsrppvptm saltptwkph sseprrngik smvaevappa qstpsvcdra ysvhl
//
LOCUS       XP_077524338             151 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135859 [Amblyomma americanum].
ACCESSION   XP_077524338
VERSION     XP_077524338.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668212.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..151
                     /product="uncharacterized protein LOC144135859"
                     /calculated_mol_wt=15786
     CDS             1..151
                     /gene="LOC144135859"
                     /coded_by="XM_077668212.1:160..615"
                     /db_xref="GeneID:144135859"
ORIGIN      
        1 mmhelgdvdh fcgledhilh pgvlplaats sagdcvigav faawvafdss ppsvsaahki
       61 ladahavvtg hksdyvvnlv nwfllvdala lnhglvqtll egwerlgvss qpllkrligs
      121 apatgidnlf iapeasralv sscpqtfqtk a
//
LOCUS       XP_077524339             168 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135913 [Amblyomma americanum].
ACCESSION   XP_077524339
VERSION     XP_077524339.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668213.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..168
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..168
                     /product="uncharacterized protein LOC144135913"
                     /calculated_mol_wt=18034
     CDS             1..168
                     /gene="LOC144135913"
                     /coded_by="XM_077668213.1:89..595"
                     /db_xref="GeneID:144135913"
ORIGIN      
        1 mrrrrsaalw lqlrdlkmmh elgdvdhfcg ledhilhpgv lplvatssag drvigavfaa
       61 wvafdsspps vsaahkilad ahavvtghks dyvvnlvnwf llvdalalnh glvqtllegw
      121 erlgvssqpq lkrligsapa tgidnlfiap easralvssc pqtfqtka
//
LOCUS       XP_077524340             440 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135929 [Amblyomma americanum].
ACCESSION   XP_077524340
VERSION     XP_077524340.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668214.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..440
                     /product="uncharacterized protein LOC144135929"
                     /calculated_mol_wt=48885
     Region          13..68
                     /region_name="MIT"
                     /note="domain contained within Microtubule Interacting and
                     Trafficking molecules. The MIT domain is found in sorting
                     nexins, the nuclear thiol protease PalBH, the AAA protein
                     spastin and archaebacterial proteins with similar domain
                     architecture, vacuolar...; cl00299"
                     /db_xref="CDD:469712"
     Region          114..>161
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Region          145..277
                     /region_name="AAA"
                     /note="ATPase family associated with various cellular
                     activities (AAA); pfam00004"
                     /db_xref="CDD:459627"
     CDS             1..440
                     /gene="LOC144135929"
                     /coded_by="XM_077668214.1:80..1402"
                     /db_xref="GeneID:144135929"
ORIGIN      
        1 mvipvtlind ikqavdlskq ahaadnkeqy anairlyrka mesylnaall ignscapfie
       61 raqqlkaffg sdanedvvve gmpemkkihq iavlsqvlpp wrtaagtvts gkkinfcdgi
      121 qgidpaklll tqiladpkak aastillygp hgtgksllvk slyvkypekf ifivdldffv
      181 igglthdpal mtrmlvrnym kyntavlile gldrlylpea peakkpvvda vkaellaymk
      241 elvekkeyke vviatasqpw riaedmrstf eakinvpipe seearkaiik aelfklrvpc
      301 yragdigsla kntnrfsryq llriircala rnfnkieaie ikenaerlsh mtkadmdkva
      361 plftadlsee depkhqefia annpapaaiv lplllppmps lhlllrplll lllpllllll
      421 plllllprpp plqlllpisg
//
LOCUS       XP_077524341             165 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144135999 [Amblyomma americanum].
ACCESSION   XP_077524341
VERSION     XP_077524341.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668215.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..165
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..165
                     /product="uncharacterized protein LOC144135999"
                     /calculated_mol_wt=18279
     CDS             1..165
                     /gene="LOC144135999"
                     /coded_by="XM_077668215.1:86..583"
                     /db_xref="GeneID:144135999"
ORIGIN      
        1 meendlrrak ahqvlpqiis smeegsaehg gtygkhiral rllhvlltpg aevtlthsnd
       61 gseeggpcqq envaqgpeec gfsveedknh kkdpevprrs tavrdarlih kevlrhppai
      121 sllsqkatkn fhvshsarps nrkrmahhak ernrrssrva ccqla
//
LOCUS       XP_077524342             267 aa            linear   INV 25-NOV-2025
DEFINITION  cysteine-rich secretory protein 2-like [Amblyomma americanum].
ACCESSION   XP_077524342
VERSION     XP_077524342.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668216.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..267
                     /product="cysteine-rich secretory protein 2-like"
                     /calculated_mol_wt=30844
     Region          49..190
                     /region_name="CAP"
                     /note="CAP (cysteine-rich secretory proteins, antigen 5,
                     and pathogenesis-related 1 proteins) domain family;
                     cl00133"
                     /db_xref="CDD:412178"
     Region          207..267
                     /region_name="Crisp"
                     /note="pfam08562"
                     /db_xref="CDD:462519"
     CDS             1..267
                     /gene="LOC144136026"
                     /coded_by="XM_077668216.1:176..979"
                     /db_xref="GeneID:144136026"
ORIGIN      
        1 mrnlslqtts lwllllefcs ggkipvlprp kvygnmlpkr sldtryrpvm krivlyhnff
       61 rtrvdppasd mlemtwhkga aadaqrwaes cqllvhdnss grwvedygsc gqnifvsnvr
      121 vpwffaikiw flehhnfsfg dtgnnipsmv ghytqmvwys shkvgcglhy cgrnvvkkpf
      181 ynyvcnycpi gnfperfgkp ytkgkpcskc pghcrskklc tnscehadfw vncreiakhw
      241 hqwlcgnpak eqykacratc sckerik
//
LOCUS       XP_077524343             178 aa            linear   INV 25-NOV-2025
DEFINITION  coactosin-like protein isoform X1 [Amblyomma americanum].
ACCESSION   XP_077524343
VERSION     XP_077524343.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668217.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..178
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..178
                     /product="coactosin-like protein isoform X1"
                     /calculated_mol_wt=19914
     Region          64..157
                     /region_name="ADF_coactosin_like"
                     /note="Coactosin-like members of the ADF homology domain
                     family; cd11282"
                     /db_xref="CDD:200438"
     Site            order(110,112,126,128,139,153,156)
                     /site_type="other"
                     /note="putative F-actin binding interface [polypeptide
                     binding]"
                     /db_xref="CDD:200438"
     CDS             1..178
                     /gene="LOC144136053"
                     /coded_by="XM_077668217.1:120..656"
                     /db_xref="GeneID:144136053"
ORIGIN      
        1 mqtadgrrge aslallcstp svdrialrta drhvcphrvc prrlrrrqgr pqqhlldvrl
       61 crralltydg gqiratskge dfetlkknls eherafvylr iqagdemskr ckfvfitwms
      121 esvgvlqrar issdkaavke vitnfavelq vedpaelted nikntvnkag gtdygtas
//
LOCUS       XP_077524344             139 aa            linear   INV 25-NOV-2025
DEFINITION  coactosin-like protein isoform X2 [Amblyomma americanum].
ACCESSION   XP_077524344
VERSION     XP_077524344.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668218.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..139
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..139
                     /product="coactosin-like protein isoform X2"
                     /calculated_mol_wt=15397
     Region          7..118
                     /region_name="ADF_coactosin_like"
                     /note="Coactosin-like members of the ADF homology domain
                     family; cd11282"
                     /db_xref="CDD:200438"
     Site            order(71,73,87,89,100,114,117)
                     /site_type="other"
                     /note="putative F-actin binding interface [polypeptide
                     binding]"
                     /db_xref="CDD:200438"
     CDS             1..139
                     /gene="LOC144136053"
                     /coded_by="XM_077668218.1:125..544"
                     /db_xref="GeneID:144136053"
ORIGIN      
        1 msvhiesvrg ayddvrddrn stswalltyd ggqiratskg edfetlkknl seherafvyl
       61 riqagdemsk rckfvfitwm sesvgvlqra rissdkaavk evitnfavel qvedpaelte
      121 dnikntvnka ggtdygtas
//
LOCUS       XP_077524345             154 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136079 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077524345
VERSION     XP_077524345.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668219.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..154
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..154
                     /product="uncharacterized protein LOC144136079 isoform X1"
                     /calculated_mol_wt=16563
     CDS             1..154
                     /gene="LOC144136079"
                     /coded_by="XM_077668219.1:70..534"
                     /db_xref="GeneID:144136079"
ORIGIN      
        1 mtipviltal liasctncgt gstnehlgla edspehhggs artndhrlkr agaydptnfn
       61 psgnigekgk vlkcetegyk ctpmdscqhw ifkpflfgcq hgqvcceksp gnicagqgsa
      121 ypgrcvpknt grcmsrrpas dcklnemccr nrnk
//
LOCUS       XP_077524346             113 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136079 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077524346
VERSION     XP_077524346.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668220.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..113
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..113
                     /product="uncharacterized protein LOC144136079 isoform X2"
                     /calculated_mol_wt=11842
     CDS             1..113
                     /gene="LOC144136079"
                     /coded_by="XM_077668220.1:70..411"
                     /db_xref="GeneID:144136079"
ORIGIN      
        1 mtipviltal liasctncgt gstnehlgla edspehhggs artndhrlkr agaydptnfn
       61 psgnigespg nicagqgsay pgrcvpkntg rcmsrrpasd cklnemccrn rnk
//
LOCUS       XP_077524347             183 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136107 [Amblyomma americanum].
ACCESSION   XP_077524347
VERSION     XP_077524347.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668221.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="uncharacterized protein LOC144136107"
                     /calculated_mol_wt=21454
     CDS             1..183
                     /gene="LOC144136107"
                     /coded_by="XM_077668221.1:236..787"
                     /db_xref="GeneID:144136107"
ORIGIN      
        1 mswdahvskv clslsryigi vsrnkhilpa ktklnlyyaf fysyvsycfl vwgqttvsni
       61 qkllvlqkri lrvianvpyd aptrpiyqqy rmipvtrmfd yrfssfykki iaeddaffsg
      121 ivtltphrtp yvtrqrmiys lprcrtnygf stlkyilpky lntpfydtlh smsvsairnv
      181 fss
//
LOCUS       XP_077524348             571 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136123 [Amblyomma americanum].
ACCESSION   XP_077524348
VERSION     XP_077524348.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668222.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..571
                     /product="uncharacterized protein LOC144136123"
                     /calculated_mol_wt=64708
     CDS             1..571
                     /gene="LOC144136123"
                     /coded_by="XM_077668222.1:29..1744"
                     /db_xref="GeneID:144136123"
ORIGIN      
        1 mrqqgllkws dftltvntdg splfksnnss vwpiqvcvne lpqphrmens flaglwfgrk
       61 hpdmmlfvge fvkavqavge vvwqhgaaav askvyiacvc vdaparasvg nhtqfnglfg
      121 cpwclacgkl eegrrlytst eppaeertsk gvrknmklam lqktsvnglk gpsplwslky
      181 ldlvscytve ymhcvllgvt rqfteywfdp srcrekyyig rpstvnalnk rltsirpphh
      241 vtrlsrtiqe rhfwkahewr nwllfycvpc clntlpaqfl rhfallceai fillqeqlss
      301 aaisyadrll qrfvcraaal ygercmsfnv hqlthltqaa rnfgplwahs afgfesgngh
      361 ivklvtaang vpdqilerai msqelhlvls lnaiplrsqq lcseflhypn vskahyvqga
      421 cmfgvpkevp lllpaeraal elmlnniprv qeyfrffyrg tvmhsqqyqr gtksdssvie
      481 sqdgeffvik rifevvdegs psngevvllc rkivcedcdl ylpghimqcf fslefplval
      541 nlsgvakpci fvtfsneeny vckipnlier d
//
LOCUS       XP_077524349             403 aa            linear   INV 25-NOV-2025
DEFINITION  melatonin receptor type 1B-like [Amblyomma americanum].
ACCESSION   XP_077524349
VERSION     XP_077524349.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668223.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..403
                     /product="melatonin receptor type 1B-like"
                     /calculated_mol_wt=44588
     CDS             1..403
                     /gene="LOC144136150"
                     /coded_by="XM_077668223.1:140..1351"
                     /db_xref="GeneID:144136150"
ORIGIN      
        1 mgasaaslfl ellkrvpqqp srqqqsslps vtytapvfph penssarage epvlvwmpyq
       61 nnalmslvly gvlavlgttl nifqisslfi qenlrrkgnv llanlalanl lvssvgfpvt
      121 ivailagsqg slltcrwqwy pallgfyttv ftflfiaaen yvrscrqdrd vyssvcgakl
      181 vvflvlaawt iaislvlvvt lvtggldvce rdmreskmfv alcvpfaltv avfakamheq
      241 rdytynlelr nclatreeil qrtllrtnti ayalflllwl pflltviisp smrssspgts
      301 avetlfftaq cfscicgcvy afthdafrqg flylvhyfcc kshpefskpp igddkatssi
      361 rvhvgmdsrv gerrhrygka atmgllsfag gdacrtrvee dfl
//
LOCUS       XP_077524351             463 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136166 [Amblyomma americanum].
ACCESSION   XP_077524351
VERSION     XP_077524351.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668225.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..463
                     /product="uncharacterized protein LOC144136166"
                     /calculated_mol_wt=51048
     CDS             1..463
                     /gene="LOC144136166"
                     /coded_by="XM_077668225.1:60..1451"
                     /db_xref="GeneID:144136166"
ORIGIN      
        1 mssptppltp tppltptppl tpspvsseeq tgavregatn adgdaaaavv geapyrcrhw
       61 platlvlaal qlhlhwqaay rhdqsrcasl atiledghws rvvsaafhhe drwqlavnvt
      121 sflwkgsrle msigtplfav amaklvllvg vtntllnlaa glvlntdeyd tacmytfsgv
      181 lvalkiinek rrdftrfrwg tvevavpppr iawlevialh lassrslttm fsgllvgllf
      241 adgivsprqn ggagslfkap flpatysimg aflathffwp gldpcvsfra vfeqrrwqql
      301 llpslytfss yhlvyvvlsl lslgrqmepr yghdnflllv lfvalcvnsa hclitwilsr
      361 hslklfepll ppslrdtcfs gftgalltlk mllykerpga dydfasfqma vphwfgfite
      421 vahlymytar awllghaagi avgltlihls glrppdlarr rls
//
LOCUS       XP_077524363             234 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136176 [Amblyomma americanum].
ACCESSION   XP_077524363
VERSION     XP_077524363.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668237.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..234
                     /product="uncharacterized protein LOC144136176"
                     /calculated_mol_wt=24087
     CDS             1..234
                     /gene="LOC144136176"
                     /coded_by="XM_077668237.1:45..749"
                     /db_xref="GeneID:144136176"
ORIGIN      
        1 mpgvvtqkpg lgappsspls aaaehvdpgr tykrphpaas pdtrtpslll lpvdiprpka
       61 dagmssqlrl aavaltlllv aclvppteqs kklkalklga aaaaaavgki tplalaggaa
      121 gaslvgsaav shlinshhhh hggyhhhhhf ipvhhshhvp iplpypvhth hdhhhhhglv
      181 dfgyadhgfg hghghghghg hgffadyghg fgggfggglg gfgygghgdy dgww
//
LOCUS       XP_077524389             246 aa            linear   INV 25-NOV-2025
DEFINITION  exosome complex component Ski6 [Amblyomma americanum].
ACCESSION   XP_077524389
VERSION     XP_077524389.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668263.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..246
                     /product="exosome complex component Ski6"
                     /calculated_mol_wt=26903
     Region          11..236
                     /region_name="RNase_PH_RRP41"
                     /note="RRP41 subunit of eukaryotic exosome; cd11370"
                     /db_xref="CDD:206775"
     Site            order(34,36,51,53..55,73,75,80,98,101,105,119,124,129,
                     147..149,156,196..207,210,214)
                     /site_type="other"
                     /note="hexamer interface [polypeptide binding]"
                     /db_xref="CDD:206775"
     Site            order(36,54..57,59,112,116,119,147..150,152..154,156,229,
                     232..233,236)
                     /site_type="other"
                     /note="Rrp4 interface [polypeptide binding]"
                     /db_xref="CDD:206775"
     CDS             1..246
                     /gene="Ski6"
                     /coded_by="XM_077668263.1:51..791"
                     /db_xref="GeneID:144136186"
ORIGIN      
        1 magleflsde gyrldgrkps eqrkiacrlg vfgqadgsay ieqgnakvla avygpheprg
       61 srsrsfhdra lvncqfsmat fstlerkrrp rgdkksqemt lhiqqafeas iltqlyprsq
      121 idifvetlqs dggtlsvcin aatlalidag ialkdyvcac svgfvddvpl vdishleesm
      181 rgpeltvavl pksqemvlle mtsrvhvdnl qkmldagvkg ctdvhaifds rvkehtaqvg
      241 scigwe
//
LOCUS       XP_077524405             832 aa            linear   INV 25-NOV-2025
DEFINITION  neural cell adhesion molecule 1-like [Amblyomma americanum].
ACCESSION   XP_077524405
VERSION     XP_077524405.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668279.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..832
                     /product="neural cell adhesion molecule 1-like"
                     /calculated_mol_wt=89835
     Region          41..154
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          51..55
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          70..74
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          118..123
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          133..138
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          151..154
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          155..261
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          184..188
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          199..203
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          238..243
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          254..257
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          294..366
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          300..304
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          314..318
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          354..359
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          391..477
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          404..408
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          418..422
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          440..444
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          461..466
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          474..477
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..832
                     /gene="LOC144136190"
                     /coded_by="XM_077668279.1:197..2695"
                     /db_xref="GeneID:144136190"
ORIGIN      
        1 mhaswtlvtl lswtlllgch gtllryhdqd dpapdeedea psyvgqeggq velpcsslha
       61 pgasqaspvr vewhrrgdad pdpvyavhfs granlmqgrh qsrhdwapra yfavlgspaa
      121 lkvkalglad aglytcrvtr tdgssqastv rlsvlgplep pvirdesgni lndtagpyme
      181 gdvatlvcqv kaagrpapev rwlqrpqrps sdddeervpa savttvtsal ddaqgsvlvl
      241 sklrqgplar tdlltqlvcq vdnglaapvr ttvridmrla pltvrilras ggaltaglpv
      301 elvceargsr ppaqltwwkr gvqlaqsfgh asadgnvsts vvsftpsaqd hgqglrcvar
      361 npalptsapa pqdrwamnvf ykpnvtlrlg qpfreleiqe ggdvylecvv nanppsaevh
      421 wskdgvedfa trtlngstlp dvvisgrfla lqkarrsfsg ryacsatnse gtttsnslrl
      481 kvlhaptcve gapivysasr hepvrvtcrv aaspeavhfr wsfssstrrt eltdfvvlgp
      541 eadpdgtpvl dgdsptvvsv leytphfqsd fgtllcsarn skgdqrepct fhvvqagppe
      601 pvencsvsnl tersfsvecr evrassasgq savaatggas lvaaaqrpsl larppenlvy
      661 llevygppeg sslqrgvsap qhqvlvrnvs gpeprfrvgq lwpgtefear vfaanskgrs
      721 rpfrlnactl ppaealldkv ptwtlgfsil hwlllvlfli livvavillv wfrrrmqgna
      781 tegttkkttt dvqepwcgqm aqtvpvsrsr ssiistkdei mvldkrkdtv dv
//
LOCUS       XP_077524439             182 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136208 [Amblyomma americanum].
ACCESSION   XP_077524439
VERSION     XP_077524439.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668313.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..182
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..182
                     /product="uncharacterized protein LOC144136208"
                     /calculated_mol_wt=20111
     Region          11..167
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..182
                     /gene="LOC144136208"
                     /coded_by="XM_077668313.1:44..592"
                     /db_xref="GeneID:144136208"
ORIGIN      
        1 mecdalrssf ngllaedqlr vlqkgtmrgs swtqktiqes lkvrlacggr gyeylrgngw
       61 pltaertlqk hiedikftpg ileevfpala skvatfhnee ryavlmldei qltpgldydi
      121 ttrsvigrpt lpssdpsaep vlathalvfm lggiasrwkq tvayhftgtv wpipkelhag
      181 dv
//
LOCUS       XP_077524474             146 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136221 [Amblyomma americanum].
ACCESSION   XP_077524474
VERSION     XP_077524474.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668348.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..146
                     /product="uncharacterized protein LOC144136221"
                     /calculated_mol_wt=14459
     CDS             1..146
                     /gene="LOC144136221"
                     /coded_by="XM_077668348.1:211..651"
                     /db_xref="GeneID:144136221"
ORIGIN      
        1 maphvcnpmc licggphlts srectakfik lpqpgprtps kgansgatrg grqqnnqktp
       61 rrgvmpppgk tatvtpppga aefpalgasp ggaacnqpkr glgggparls rpeepkglgs
      121 tdaggvacqw gpgigappst vrgkgq
//
LOCUS       XP_077524489             800 aa            linear   INV 25-NOV-2025
DEFINITION  neprilysin-1-like [Amblyomma americanum].
ACCESSION   XP_077524489
VERSION     XP_077524489.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668363.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..800
                     /product="neprilysin-1-like"
                     /calculated_mol_wt=91655
     Region          139..797
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..800
                     /gene="LOC144136225"
                     /coded_by="XM_077668363.1:15..2417"
                     /db_xref="GeneID:144136225"
ORIGIN      
        1 mepspralyq evgvrstspe tkccvvrtrt rsrtihtshr rlpyivvcav gmvmvlvgva
       61 vstyklqnrs tildlfnrpm slfiynataa gqddsedaah psshllkhqv rlpdtdttct
      121 tetclwmery lrgklnssvn pcvdfysyvc sskwfskgla irdrpymert vgmlmldvek
      181 ffraylkqne dkyhkypgvf vhqaisllsk cqseekpdkn ftalrsllee ynlggwpyrk
      241 aprgvsmvti cafvdrdlgv fsfarvylkk pfeddrgytv hldkpsstle rhqlaflses
      301 venytnrvdm alslfetkhd mseqaeaiva leqklaaamp vpkftefqdr ikpvghldrr
      361 gkwnwkeyln iifqdikvfd nekpvavmhq dyllkmasiv netetvtlln yigyrlvvqv
      421 spllakpagq llrmshdnyq efvperlqac mhllervykq gmrfftrmtf skdnstlllk
      481 hydysmsnve aeikssmadr llsesswldr aaigigvdkl esmrlvflgs sddintvany
      541 ynfnaqpldp thllesfrev qaatmsiywe tkppkddlda rydhstlspg yeyffgrnvl
      601 fvphgniafv ndisknidpv lyplllpnvl rgmfsavdrr gatvdhnsav stwwntdels
      661 kfsqmqlcfq dqyyielrel vgdsfdarik ldeniadsai laplhdiylk altsqgttle
      721 kqklpvdige vdmhkmffis yalglcdnps kessarkvky eripgrlrin iplmnfakfs
      781 tafncpvnsp mnparkcavw
//
LOCUS       XP_077524505             205 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136226 [Amblyomma americanum].
ACCESSION   XP_077524505
VERSION     XP_077524505.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668379.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..205
                     /product="uncharacterized protein LOC144136226"
                     /calculated_mol_wt=22196
     CDS             1..205
                     /gene="LOC144136226"
                     /coded_by="XM_077668379.1:135..752"
                     /db_xref="GeneID:144136226"
ORIGIN      
        1 mtgyhctliv lgltvgaawg fehtisegvt pgapavtgng tgqelsgveg ergsvlravq
       61 rwfqnsleky rlrfttttvs restsessah thmplgftrd pvcnmsaflk qarlcwktaa
      121 vysdkpdgic ahkvdamyrr clvrvtkqts ceederaqqr irgevdsqra qcvgasfpra
      181 aaspwgfgap slgrfilatl lvlal
//
LOCUS       XP_077524518             589 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136230 [Amblyomma americanum].
ACCESSION   XP_077524518
VERSION     XP_077524518.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668392.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..589
                     /product="uncharacterized protein LOC144136230"
                     /calculated_mol_wt=67409
     CDS             1..589
                     /gene="LOC144136230"
                     /coded_by="XM_077668392.1:105..1874"
                     /db_xref="GeneID:144136230"
ORIGIN      
        1 mffslagprr tglvllllva latgrrhyrs tedddedeee dqgwgddrgk rrrldpkpaq
       61 essghsgfsy edqdqsppka ecevdsfrrg qsecdrilrm nlphhhgshd yvcyslrkyk
      121 mcvasvmiqs scyertfisq elhkirgiiq gysvdcfnit ketnrrglkv rrlrfrmakv
      181 klasecspaa awaveltctk dfehdmkvlg qlnepsravk kscrtilryy hcltpvtest
      241 scqknpefik hleyfpkait aryqevclke ldispslfke kvntsmaavk aqadgckeee
      301 asreflacal vfnevvahsa dkfpqceayk nfiqctemia ktlncgqnse fhyhavhvlq
      361 vllhdyqekc knrwkrgrsn erqfpsgegr psdsdnsgrv ggadgrssde rpsgnryast
      421 arpeqpegsy trrrffkhte qvfrittenp fypplrklpf eprvpqaedy prshsessse
      481 lernrprsen dderggrsnr rarpgrrggt rhvgaedgnn gegggrtayr dsqgdsgghr
      541 nrlnnrntkr depdkkddgy qpdpndgfle dsedveairr rkkklierl
//
LOCUS       XP_077524532             300 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136237 [Amblyomma americanum].
ACCESSION   XP_077524532
VERSION     XP_077524532.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668406.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..300
                     /product="uncharacterized protein LOC144136237"
                     /calculated_mol_wt=34894
     CDS             1..300
                     /gene="LOC144136237"
                     /coded_by="XM_077668406.1:192..1094"
                     /db_xref="GeneID:144136237"
ORIGIN      
        1 mwerfpvyll lvvcsikaes epirrrpfas patgclenrl lrlrlecesa ldevarlrmk
       61 rdqsescrkl rkyitcidia ltstkcrrdr yvfgreverr krqvntynwd cslqmqnydy
      121 vmcdsktlin rhlacgttfd rsivnlnfel dedkeklcss ltdykhcvtp likndacssn
      181 knlisrllhf sravvgeyqt scktiamkhi mearqvedta clpedlrekv lscqqsayrt
      241 irardvhset diceeldlmk ecidnalket scsrdapsrr vandttaesy arynvkcrrf
//
LOCUS       XP_077524554              59 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like [Amblyomma americanum].
ACCESSION   XP_077524554
VERSION     XP_077524554.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668428.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..59
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..59
                     /product="tubulin beta chain-like"
                     /calculated_mol_wt=6764
     Region          <1..39
                     /region_name="Tubulin_FtsZ_Cetz-like"
                     /note="Tubulin protein family of FtsZ and CetZ-like;
                     cl10017"
                     /db_xref="CDD:471962"
     CDS             1..59
                     /gene="LOC144136246"
                     /coded_by="XM_077668428.1:6..185"
                     /db_xref="GeneID:144136246"
ORIGIN      
        1 mfrrkaflhw ytgegmdeme fteaesnmnd lvseyqqyqe ataddegefd dedaladta
//
LOCUS       XP_077524568              59 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like [Amblyomma americanum].
ACCESSION   XP_077524568
VERSION     XP_077524568.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668442.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..59
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..59
                     /product="tubulin beta chain-like"
                     /calculated_mol_wt=6764
     Region          <1..39
                     /region_name="Tubulin_FtsZ_Cetz-like"
                     /note="Tubulin protein family of FtsZ and CetZ-like;
                     cl10017"
                     /db_xref="CDD:471962"
     CDS             1..59
                     /gene="LOC144136248"
                     /coded_by="XM_077668442.1:6..185"
                     /db_xref="GeneID:144136248"
ORIGIN      
        1 mfrrkaflhw ytgegmdeme fteaesnmnd lvseyqqyqe ataddegefd dedaladta
//
LOCUS       XP_077524594             154 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain [Amblyomma americanum].
ACCESSION   XP_077524594
VERSION     XP_077524594.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668468.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..154
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..154
                     /product="tubulin beta chain"
                     /calculated_mol_wt=17586
     Region          <1..138
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..154
                     /gene="LOC144136262"
                     /coded_by="XM_077668468.1:18..482"
                     /db_xref="GeneID:144136262"
ORIGIN      
        1 mfdaknmmaa cdprhgrylt vatifrgrms mkevdeqmln vqnknssyfv ewipnnvkta
       61 vcdipprglk msatfignst aiqelfkris eqftamfrrk aflhwytgeg mdemefteae
      121 snmndlvsey qqyqeatadd egefddedal adta
//
LOCUS       XP_077524608             300 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like [Amblyomma americanum].
ACCESSION   XP_077524608
VERSION     XP_077524608.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668482.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..300
                     /product="tubulin beta chain-like"
                     /calculated_mol_wt=34018
     Region          <1..284
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..300
                     /gene="LOC144136268"
                     /coded_by="XM_077668482.1:1..903"
                     /db_xref="GeneID:144136268"
ORIGIN      
        1 mgtlliskir eeypdrimnt fsvmpspkvs dtvvgpynat lsvhqlvent detycidnea
       61 lydicfrtlk lttptygdln hlvsvtmsgv ttclrfpgql nadlrklavn mvpfprlhff
      121 mpgfapltsr gsqqyraltv peltqqmfda knmmaacdpr hgryltvati frsrmsmkev
      181 deqmlnvqnk nssyfvewip nnvktavcdi pprglkmsat fignstaiqe lfkriseqft
      241 amfrrkaflh wytgegmdem efteaesnmn dlvseyqqyq eataddegef ddedaladta
//
LOCUS       XP_077524621             300 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like [Amblyomma americanum].
ACCESSION   XP_077524621
VERSION     XP_077524621.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668495.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..300
                     /product="tubulin beta chain-like"
                     /calculated_mol_wt=34060
     Region          <1..284
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..300
                     /gene="LOC144136277"
                     /coded_by="XM_077668495.1:58..960"
                     /db_xref="GeneID:144136277"
ORIGIN      
        1 mgtlliskir eeypdrimnt fsvmpspkvs dtvvepynat lsvhqlvent detycidnea
       61 lydicfrtlk lttptygdln hlvsvtmsgv ttclrfpgql nadlrklavn mvpfprlhff
      121 mpgfapltsr gsqqyraltv peltqqmfda knmmaacdpr hgryltvati frgrmsmkev
      181 deqmlnvqnk nssyfvewip nnvktavcdi pprglkmsat fignstaiqe lfkriseqft
      241 amfrrkaflh wytgegmdem efteaesnmn dlvseyqqyq eataddegef ddedaladta
//
LOCUS       XP_077524633              59 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like [Amblyomma americanum].
ACCESSION   XP_077524633
VERSION     XP_077524633.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668507.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..59
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..59
                     /product="tubulin beta chain-like"
                     /calculated_mol_wt=6764
     Region          <1..39
                     /region_name="Tubulin_FtsZ_Cetz-like"
                     /note="Tubulin protein family of FtsZ and CetZ-like;
                     cl10017"
                     /db_xref="CDD:471962"
     CDS             1..59
                     /gene="LOC144136284"
                     /coded_by="XM_077668507.1:6..185"
                     /db_xref="GeneID:144136284"
ORIGIN      
        1 mfrrkaflhw ytgegmdeme fteaesnmnd lvseyqqyqe ataddegefd dedaladta
//
LOCUS       XP_077524646              59 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like [Amblyomma americanum].
ACCESSION   XP_077524646
VERSION     XP_077524646.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668520.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..59
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..59
                     /product="tubulin beta chain-like"
                     /calculated_mol_wt=6764
     Region          <1..39
                     /region_name="Tubulin_FtsZ_Cetz-like"
                     /note="Tubulin protein family of FtsZ and CetZ-like;
                     cl10017"
                     /db_xref="CDD:471962"
     CDS             1..59
                     /gene="LOC144136291"
                     /coded_by="XM_077668520.1:6..185"
                     /db_xref="GeneID:144136291"
ORIGIN      
        1 mfrrkaflhw ytgegmdeme fteaesnmnd lvseyqqyqe ataddegefd dedaladta
//
LOCUS       XP_077524659              59 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like [Amblyomma americanum].
ACCESSION   XP_077524659
VERSION     XP_077524659.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668533.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..59
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..59
                     /product="tubulin beta chain-like"
                     /calculated_mol_wt=6764
     Region          <1..39
                     /region_name="Tubulin_FtsZ_Cetz-like"
                     /note="Tubulin protein family of FtsZ and CetZ-like;
                     cl10017"
                     /db_xref="CDD:471962"
     CDS             1..59
                     /gene="LOC144136296"
                     /coded_by="XM_077668533.1:6..185"
                     /db_xref="GeneID:144136296"
ORIGIN      
        1 mfrrkaflhw ytgegmdeme fteaesnmnd lvseyqqyqe ataddegefd dedaladta
//
LOCUS       XP_077524671             374 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like [Amblyomma americanum].
ACCESSION   XP_077524671
VERSION     XP_077524671.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668545.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..374
                     /product="tubulin beta chain-like"
                     /calculated_mol_wt=41817
     Region          1..358
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..374
                     /gene="LOC144136301"
                     /coded_by="XM_077668545.1:49..1173"
                     /db_xref="GeneID:144136301"
ORIGIN      
        1 mdsvrsgpfg qlfrpdnfif gqsgagnnla kghytegael vdsvldvvrk eaescdclqg
       61 fqlahslggg tgsgmgtlli skireeypdr imntfsvmps pkvsdtvvep ynatlsvhql
      121 ventdetyci dnealydicf rtlklttpty gdlnhlvsvt msgvttclrf pgqlnadlrk
      181 lavnmvpfpr lhffmpgfap ltsrgsqqyr altvpeltqq mfdaknmmaa cdprhgrylt
      241 vatifrgrms mkevdeqmln vqnknssyfv ewipnnvkta vcdipprglk msatfignst
      301 aiqelfkris eqftamfrrk aflhwytkeg mdemefteae snmndlvsey qqyqeatadd
      361 egefddedal adta
//
LOCUS       XP_077524683              59 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like [Amblyomma americanum].
ACCESSION   XP_077524683
VERSION     XP_077524683.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668557.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..59
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..59
                     /product="tubulin beta chain-like"
                     /calculated_mol_wt=6764
     Region          <1..39
                     /region_name="Tubulin_FtsZ_Cetz-like"
                     /note="Tubulin protein family of FtsZ and CetZ-like;
                     cl10017"
                     /db_xref="CDD:471962"
     CDS             1..59
                     /gene="LOC144136310"
                     /coded_by="XM_077668557.1:6..185"
                     /db_xref="GeneID:144136310"
ORIGIN      
        1 mfrrkaflhw ytgegmdeme fteaesnmnd lvseyqqyqe ataddegefd dedaladta
//
LOCUS       XP_077524729             120 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136345 [Amblyomma americanum].
ACCESSION   XP_077524729
VERSION     XP_077524729.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668603.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..120
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..120
                     /product="uncharacterized protein LOC144136345"
                     /calculated_mol_wt=12976
     CDS             1..120
                     /gene="LOC144136345"
                     /coded_by="XM_077668603.1:198..560"
                     /db_xref="GeneID:144136345"
ORIGIN      
        1 msslfpsgyt shdisptavs dlakkkdywr ggkdekafys aqvmelgvtk aeliqrvskr
       61 kitamdfees epattvaqih esaavgpcvt ntvhrnlhrl atppfnssla vavvsdrrgl
//
LOCUS       XP_077524739             467 aa            linear   INV 25-NOV-2025
DEFINITION  carboxypeptidase Q-like [Amblyomma americanum].
ACCESSION   XP_077524739
VERSION     XP_077524739.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668613.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..467
                     /product="carboxypeptidase Q-like"
                     /calculated_mol_wt=50398
     Region          37..460
                     /region_name="Zinc_peptidase_like"
                     /note="Zinc peptidases M18, M20, M28, and M42; cl14876"
                     /db_xref="CDD:472712"
     CDS             1..467
                     /gene="LOC144136353"
                     /coded_by="XM_077668613.1:325..1728"
                     /db_xref="GeneID:144136353"
ORIGIN      
        1 mvnpllpllf sallsrsglp aercelspel aaeidgygpv vrrilsafda gpqsgttyra
       61 ladfadrfgh rlvgtdslea aidhlvsalr aagvdrvete pvatpvwlrg keratlvepr
      121 rvplavlglg gsvatppggl eapvlvvrsf deldarrdqv ngtivlfnpe wtdywqyvpy
      181 rskgpsraar ygavatlvrs aapaslytlh tgmlaytpge pkvpaaavti edadfltrlv
      241 ergskvvvhl emnnnhtdgv srnivaditg sefpdqyviv gahtdswdvg qgvvddaggv
      301 figlaamaql rrmhlrprrt lrlvlwtaee nglwggaefv rqhrremdav slaiesdqgt
      361 fapvglttgg gsnttvcilr hllaltapig atrleadrgr gsdvtkmael gvpvslllnr
      421 neryfhyhhs padtvsaysr rdldlcaafl taiafvvadl rdmlpra
//
LOCUS       XP_077524766             148 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136365 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077524766
VERSION     XP_077524766.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668640.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="uncharacterized protein LOC144136365 isoform X1"
                     /calculated_mol_wt=16759
     CDS             1..148
                     /gene="LOC144136365"
                     /coded_by="XM_077668640.1:269..715"
                     /db_xref="GeneID:144136365"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedrrr rhlnrrltat mkrlsgvhvl
       61 nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar pgevyvvhrc
      121 rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077524774              98 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136365 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077524774
VERSION     XP_077524774.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668648.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..98
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..98
                     /product="uncharacterized protein LOC144136365 isoform X2"
                     /calculated_mol_wt=10604
     CDS             1..98
                     /gene="LOC144136365"
                     /coded_by="XM_077668648.1:348..644"
                     /db_xref="GeneID:144136365"
ORIGIN      
        1 mkrlsgvhvl nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar
       61 pgevyvvhrc rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077524802             162 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136389 [Amblyomma americanum].
ACCESSION   XP_077524802
VERSION     XP_077524802.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668676.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..162
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..162
                     /product="uncharacterized protein LOC144136389"
                     /calculated_mol_wt=18103
     CDS             1..162
                     /gene="LOC144136389"
                     /coded_by="XM_077668676.1:1..489"
                     /db_xref="GeneID:144136389"
ORIGIN      
        1 mnpprsgtee eyeekekllq eisdlarefr ykcksrkaqa aankafatgv rdnacaaaar
       61 vsphhsdsyp tpsgtatdtp etasyvlqqm cndrdvvddy fttdaenfqs aqqtasanvt
      121 tdvqeeteeh epqptaemkr rkrkpktagd enheflekkm ep
//
LOCUS       XP_077524851              58 aa            linear   INV 25-NOV-2025
DEFINITION  protein stunted-like [Amblyomma americanum].
ACCESSION   XP_077524851
VERSION     XP_077524851.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668725.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..58
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..58
                     /product="protein stunted-like"
                     /calculated_mol_wt=6231
     Region          2..51
                     /region_name="ATP-synt_Eps"
                     /note="Mitochondrial ATP synthase epsilon chain;
                     pfam04627"
                     /db_xref="CDD:461373"
     Site            order(4..5,9..12,15..16,20,38..48)
                     /site_type="other"
                     /note="gamma subunit interface [polypeptide binding]"
                     /db_xref="CDD:213396"
     Site            order(11,14..19,24..27,31,38)
                     /site_type="other"
                     /note="delta subunit interface [polypeptide binding]"
                     /db_xref="CDD:213396"
     CDS             1..58
                     /gene="LOC144136421"
                     /coded_by="XM_077668725.1:155..331"
                     /db_xref="GeneID:144136421"
ORIGIN      
        1 mtywraaglt ylqfssiaar avrnslkket lqlsaaagse stikmaawkd gkpvkket
//
LOCUS       XP_077524862             118 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136428 [Amblyomma americanum].
ACCESSION   XP_077524862
VERSION     XP_077524862.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668736.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..118
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..118
                     /product="uncharacterized protein LOC144136428"
                     /calculated_mol_wt=10967
     CDS             1..118
                     /gene="LOC144136428"
                     /coded_by="XM_077668736.1:125..481"
                     /db_xref="GeneID:144136428"
ORIGIN      
        1 mnalvvaalf acvalsygqf dfgggfdgga gaggfdsssf gspdggaqlg gggdfggsfa
       61 ggdtdggaga sfdtsgftgg fegfnaaaas ssrpqfggfq sqfqgsqagg aqssveaq
//
LOCUS       XP_077524876              72 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136433 [Amblyomma americanum].
ACCESSION   XP_077524876
VERSION     XP_077524876.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668750.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..72
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..72
                     /product="uncharacterized protein LOC144136433"
                     /calculated_mol_wt=7331
     CDS             1..72
                     /gene="LOC144136433"
                     /coded_by="XM_077668750.1:105..323"
                     /db_xref="GeneID:144136433"
ORIGIN      
        1 mdkslgglsa lvasllllls ghaaeaapgy ggygghvsik vyrgpshghg yhsfapfgyh
       61 vhlppdnhgy hg
//
LOCUS       XP_077524888             436 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077524888
VERSION     XP_077524888.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668762.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..436
                     /product="tubulin beta-4 chain-like isoform X1"
                     /calculated_mol_wt=48828
     Region          1..436
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..436
                     /gene="LOC144136439"
                     /coded_by="XM_077668762.1:207..1517"
                     /db_xref="GeneID:144136439"
ORIGIN      
        1 mreilhiqtg qcgnqigakf wevisdehgi dpsgayygds dlqlerinvy yneapggkyv
       61 prailvdlep gtmdavrsgr fgqlfrpdnf vfgqsgagnn wakghytega ehvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrdgqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkdssy fvewipnnvk tavcdipprg
      361 lkmsatfign staiqelfkr iseqftamfr rkaflhwytg egmdelefte aesnlnelvs
      421 eyqqyqeata ddegef
//
LOCUS       XP_077524898             435 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077524898
VERSION     XP_077524898.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668772.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..435
                     /product="tubulin beta-4 chain-like isoform X2"
                     /calculated_mol_wt=48771
     Region          1..435
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..435
                     /gene="LOC144136439"
                     /coded_by="XM_077668772.1:207..1514"
                     /db_xref="GeneID:144136439"
ORIGIN      
        1 mreilhiqtg qcgnqigakf wevisdehgi dpsgayygds dlqlerinvy yneapgkyvp
       61 railvdlepg tmdavrsgrf gqlfrpdnfv fgqsgagnnw akghytegae hvdsvldvvr
      121 keaescdclq gfqlthslgg gtgsgmgtll iskireeypd rimntysvvp spkvsdtvve
      181 pynatlsvhq lventdetyc idnealydic frtlklttpt ygdlnhlvsa tmsgvttclr
      241 fpgqlnadlr klavnmvpfp rlhffmtgfa pltsrdgqqy raltvpeltq qmfdaknmma
      301 acdprhgryl tvaavfrgrm smrevddqml niqnkdssyf vewipnnvkt avcdipprgl
      361 kmsatfigns taiqelfkri seqftamfrr kaflhwytge gmdeleftea esnlnelvse
      421 yqqyqeatad degef
//
LOCUS       XP_077524922             436 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077524922
VERSION     XP_077524922.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668796.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..436
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=48841
     Region          1..436
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..436
                     /gene="LOC144136456"
                     /coded_by="XM_077668796.1:102..1412"
                     /db_xref="GeneID:144136456"
ORIGIN      
        1 mreilhiqtg qcgnqigakf wevisdehgi dpsgayygds dlqlerinvd yneapggkyv
       61 prailvdlep gtmdavrsgr fgqlfrpdnf vfgqsgagnn wakghytega ehvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrdcqq yraltvpeqt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkdssy fvewipnnvk tavcdipprg
      361 lkmsatfign staiqelfkr iseqftamfr rkaflhwytg egmdeleftd aesnlnelvs
      421 eyqqyqeata edegef
//
LOCUS       XP_077524936             325 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136463 [Amblyomma americanum].
ACCESSION   XP_077524936
VERSION     XP_077524936.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668810.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..325
                     /product="uncharacterized protein LOC144136463"
                     /calculated_mol_wt=35911
     Region          35..109
                     /region_name="GT1"
                     /note="myb-like, SANT family; cl23759"
                     /db_xref="CDD:474047"
     CDS             1..325
                     /gene="LOC144136463"
                     /coded_by="XM_077668810.1:159..1136"
                     /db_xref="GeneID:144136463"
ORIGIN      
        1 msdldisdfs ttpsasasda tqrakkrpag prfiftvder hvfrniiaky rdvienkrtd
       61 nttkkakdea wsqlcedyns lagtrtvcva qlrklwdnmk srwkkkksee treifrtggg
      121 tlecrpmspa telvgavadh matrlpnpfd sdgahvseav lslppaalle amvndnessq
      181 gdfssevpap tsrqaapspr lnsrpltdea lpvqeeclsa grptvlvpvv pscqsvsedi
      241 apaqiartra ggprvaaver tlapevaari kaieaedrrk eelhqldlql rrsqlaeqrl
      301 knkmqhklls ldveikkqql ealkr
//
LOCUS       XP_077524947             177 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136469 [Amblyomma americanum].
ACCESSION   XP_077524947
VERSION     XP_077524947.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668821.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..177
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..177
                     /product="uncharacterized protein LOC144136469"
                     /calculated_mol_wt=19187
     CDS             1..177
                     /gene="LOC144136469"
                     /coded_by="XM_077668821.1:760..1293"
                     /db_xref="GeneID:144136469"
ORIGIN      
        1 mvviylitav lisletfqra rsklkkaent ddiatstdqe kgrgkrkrfa vftsddsdsd
       61 epscalpppf rlrscsndgp sqrntlpssp stfeqsaarv qipapeqsev paqpavaeqp
      121 vpsteeqdih sappldnsll ilrkmcfvkt qlqkiidvll gqgsptlade dcialpl
//
LOCUS       XP_077524994             116 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136497 [Amblyomma americanum].
ACCESSION   XP_077524994
VERSION     XP_077524994.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668868.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..116
                     /product="uncharacterized protein LOC144136497"
                     /calculated_mol_wt=12633
     CDS             1..116
                     /gene="LOC144136497"
                     /coded_by="XM_077668868.1:144..494"
                     /db_xref="GeneID:144136497"
ORIGIN      
        1 mpymtlepqs atqagllvgr hrvgavmkvr akrtetgpsp gcectatsfr ypvehcpshk
       61 hlqlenafla vnicsfants vsdlpasitr lsrgkfgfyl nmfrnersga atnlcr
//
LOCUS       XP_077525007             162 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136506 [Amblyomma americanum].
ACCESSION   XP_077525007
VERSION     XP_077525007.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668881.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..162
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..162
                     /product="uncharacterized protein LOC144136506"
                     /calculated_mol_wt=17895
     CDS             1..162
                     /gene="LOC144136506"
                     /coded_by="XM_077668881.1:6..494"
                     /db_xref="GeneID:144136506"
ORIGIN      
        1 mgcfaegrls sllqclgfki lcrnarvllp wwkaertsss wrrpdmspep qsaaragllv
       61 grhrvgdvmk lwvmrtetrl fsgrernatt frypvehchf dkhlylgdaf laaniisrai
      121 tsvsdlpami trptfgmfaf plsllcldgg psatcsvaat gf
//
LOCUS       XP_077525019             159 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136513 [Amblyomma americanum].
ACCESSION   XP_077525019
VERSION     XP_077525019.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668893.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..159
                     /product="uncharacterized protein LOC144136513"
                     /calculated_mol_wt=15001
     CDS             1..159
                     /gene="LOC144136513"
                     /coded_by="XM_077668893.1:477..956"
                     /db_xref="GeneID:144136513"
ORIGIN      
        1 mrgvalsalc vlcafartea ggggygggav avatpviakt tpvlvaaskp vlpnvgpaah
       61 ylgyagnvfe qllgfsghyg lpsggggggr glvgavrlpv fsgsvllghg hgggggggga
      121 fllghgggfg ggalktykla sssygipvgl pvslahgwh
//
LOCUS       XP_077525031              89 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136523 [Amblyomma americanum].
ACCESSION   XP_077525031
VERSION     XP_077525031.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668905.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..89
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..89
                     /product="uncharacterized protein LOC144136523"
                     /calculated_mol_wt=9569
     CDS             1..89
                     /gene="LOC144136523"
                     /coded_by="XM_077668905.1:34..303"
                     /db_xref="GeneID:144136523"
ORIGIN      
        1 massegakvc wmlvwlllli faafwvaafa afwyilvyvl vpcvpaakdv aallhrgvll
       61 pyfcsdnlfn grdlnsgvqf icsggtvpa
//
LOCUS       XP_077525044             302 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136532 [Amblyomma americanum].
ACCESSION   XP_077525044
VERSION     XP_077525044.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668918.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..302
                     /product="uncharacterized protein LOC144136532"
                     /calculated_mol_wt=34401
     Region          26..>88
                     /region_name="IRF"
                     /note="Interferon Regulatory Factor (IRF); also known as
                     tryptophan pentad repeat. The family of IRF transcription
                     factors is important in the regulation of interferons in
                     response to infection by virus and in the regulation of
                     interferon-inducible genes. The...; cl00096"
                     /db_xref="CDD:469613"
     Site            order(42,79,81..82,85)
                     /site_type="other"
                     /note="DNA sequence recognition sites [nucleotide
                     binding]"
                     /db_xref="CDD:238051"
     CDS             1..302
                     /gene="LOC144136532"
                     /coded_by="XM_077668918.1:123..1031"
                     /db_xref="GeneID:144136532"
ORIGIN      
        1 mprrftgklc rflfdhlega tfgerlrwld reagvfqifw rhgngssstp eedcavfmew
       61 hhyktrrtke cspmeakqrf raamnkmkls tvsswknrpl ernfqyrkfp kddldyllkk
      121 tdqrvpaspg wspddmsdad sgmtspnfvs dsdaysepas ppgvstavfe atvgkqtcfl
      181 qhpeerwnwg valdtlatps tsepsfpple ngsreysfcd teeackwlnl qefsagsdll
      241 qdvrecrrde fdcyslpsme dgdltasipg fdkffgplvp ysdsfhgqgs sfavlnhfhs
      301 fh
//
LOCUS       XP_077525051             302 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136532 [Amblyomma americanum].
ACCESSION   XP_077525051
VERSION     XP_077525051.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668925.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..302
                     /product="uncharacterized protein LOC144136532"
                     /calculated_mol_wt=34401
     Region          26..>88
                     /region_name="IRF"
                     /note="Interferon Regulatory Factor (IRF); also known as
                     tryptophan pentad repeat. The family of IRF transcription
                     factors is important in the regulation of interferons in
                     response to infection by virus and in the regulation of
                     interferon-inducible genes. The...; cl00096"
                     /db_xref="CDD:469613"
     Site            order(42,79,81..82,85)
                     /site_type="other"
                     /note="DNA sequence recognition sites [nucleotide
                     binding]"
                     /db_xref="CDD:238051"
     CDS             1..302
                     /gene="LOC144136532"
                     /coded_by="XM_077668925.1:184..1092"
                     /db_xref="GeneID:144136532"
ORIGIN      
        1 mprrftgklc rflfdhlega tfgerlrwld reagvfqifw rhgngssstp eedcavfmew
       61 hhyktrrtke cspmeakqrf raamnkmkls tvsswknrpl ernfqyrkfp kddldyllkk
      121 tdqrvpaspg wspddmsdad sgmtspnfvs dsdaysepas ppgvstavfe atvgkqtcfl
      181 qhpeerwnwg valdtlatps tsepsfpple ngsreysfcd teeackwlnl qefsagsdll
      241 qdvrecrrde fdcyslpsme dgdltasipg fdkffgplvp ysdsfhgqgs sfavlnhfhs
      301 fh
//
LOCUS       XP_077525064             293 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136543 [Amblyomma americanum].
ACCESSION   XP_077525064
VERSION     XP_077525064.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668938.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..293
                     /product="uncharacterized protein LOC144136543"
                     /calculated_mol_wt=32866
     Region          26..>88
                     /region_name="IRF"
                     /note="Interferon Regulatory Factor (IRF); also known as
                     tryptophan pentad repeat. The family of IRF transcription
                     factors is important in the regulation of interferons in
                     response to infection by virus and in the regulation of
                     interferon-inducible genes. The...; cl00096"
                     /db_xref="CDD:469613"
     Site            order(42,79,81..82,85)
                     /site_type="other"
                     /note="DNA sequence recognition sites [nucleotide
                     binding]"
                     /db_xref="CDD:238051"
     CDS             1..293
                     /gene="LOC144136543"
                     /coded_by="XM_077668938.1:283..1164"
                     /db_xref="GeneID:144136543"
ORIGIN      
        1 mprrftgklc rflfdhlega tfgerlrwvd reagvfqifw rhgngssstp eedcavfmaw
       61 hhyknrrikk capmeakqrf raamhkmklt pvnswnkqrs eknfqyrkfp kedlgylfkk
      121 mdqrvpssps cssgevsdad serasfdvvs dsdaypepas pddvtsavsk ptvekrpfav
      181 qqteecrvpe vildspmtsl cpeaplesdn hrlsfpetdp vynwervqds svssdlfpea
      241 eddrylpsae ggtlpgtlpg fsevfgplva aptscdgqcp slavlkpldp fsi
//
LOCUS       XP_077525095             261 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136564 [Amblyomma americanum].
ACCESSION   XP_077525095
VERSION     XP_077525095.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668969.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..261
                     /product="uncharacterized protein LOC144136564"
                     /calculated_mol_wt=27507
     CDS             1..261
                     /gene="LOC144136564"
                     /coded_by="XM_077668969.1:313..1098"
                     /db_xref="GeneID:144136564"
ORIGIN      
        1 mdlrfpaspl gappavllsl errpggppaa aepaadedrr sspqiaasgf cflaavccsa
       61 ggfwasaaat rllgvvflvv gaaavlaail fvpagrdgkl pvhrralpll lalvlwavaa
      121 wiiflvaqss vhgkgvlaav aaflllfslv llrvvcrrcr hrrplkdsed avqledfhhv
      181 ntqvrdtlrf gqlrtepafa aegrppvvpt appetppqas asfstfvrep ptapaepppy
      241 dgfppdgsrc rtppppystl v
//
LOCUS       XP_077525105             436 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077525105
VERSION     XP_077525105.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077668979.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..436
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=48822
     Region          1..436
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..436
                     /gene="LOC144136570"
                     /coded_by="XM_077668979.1:100..1410"
                     /db_xref="GeneID:144136570"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gtmdavrsgr fgqlfrpdnf vfgqsgagnn wakghytega ehvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdetf cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrdcqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkdssy fvewipnnvk tavcdipprg
      361 lkmsatfign staiqelfkr iseqftamfr rkaflhwytg egmdelefte aesnlnelvs
      421 eyqqyqeata edegef
//
LOCUS       XP_077525151             446 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136576 [Amblyomma americanum].
ACCESSION   XP_077525151
VERSION     XP_077525151.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077669025.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..446
                     /product="uncharacterized protein LOC144136576"
                     /calculated_mol_wt=37979
     CDS             1..446
                     /gene="LOC144136576"
                     /coded_by="XM_077669025.1:160..1500"
                     /db_xref="GeneID:144136576"
ORIGIN      
        1 mrlavllsal vasasagalg gaslgagslg gaallggglg shalvgdaaa ltglsslvgl
       61 gglgslgagg lgagglgavg lgagglgagg lgdgalgagg lgaaglglgg lggggavvaa
      121 gptvvgvttp vavaagpatv ggpvsvsapv sipgpvatpv asvtyvkqpv iklryvtrpv
      181 ttyvrqpvat vthtikkivn ynnagallgl dgagdagldg algggngfgg gngngggggf
      241 gdgngggfgg gngggagggy aggngggngg vngggggftg gpsggagfsg ghgggagfsg
      301 gpgagglsgg hggggfggng gaggfsgngg gggfggnggg ggfggnggag gygghggggg
      361 fggnggsggf ggngggggfr rsggggyggg aggggfggps sggyggpssg syggpsasrf
      421 sggngfkpvr vsgrgpsfnv ykkhhy
//
LOCUS       XP_077525164             251 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136582 [Amblyomma americanum].
ACCESSION   XP_077525164
VERSION     XP_077525164.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077669038.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..251
                     /product="uncharacterized protein LOC144136582"
                     /calculated_mol_wt=21893
     CDS             1..251
                     /gene="LOC144136582"
                     /coded_by="XM_077669038.1:109..864"
                     /db_xref="GeneID:144136582"
ORIGIN      
        1 msalvillaw laasthagnl gggggagdlg gaaggpggqg lngggaglgp glgsgglgdg
       61 lggglggglg gglggalgag lggglggvgg ivgpavatgp vsvgapvavs rtvsipapva
      121 tpvatvsflk qpvihlkyvt kpvvsyvqqp vatvhhairp vvhvthavaa paiaatpava
      181 aapvvlgafg gpalvgglgg vglgglggvg lggaglggag lgggglglga gglglggagn
      241 avvikakhlk s
//
LOCUS       XP_077525176             175 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136587 [Amblyomma americanum].
ACCESSION   XP_077525176
VERSION     XP_077525176.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077669050.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..175
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..175
                     /product="uncharacterized protein LOC144136587"
                     /calculated_mol_wt=15591
     CDS             1..175
                     /gene="LOC144136587"
                     /coded_by="XM_077669050.1:37..564"
                     /db_xref="GeneID:144136587"
ORIGIN      
        1 mdsmfhllhv vifsalvaca sagflgglgg lggaglgglg lgggsglggg sglgggaglg
       61 glglgggfah lgsalgsasa hpvatlgvvk qpiinygvva kpvvsyvaqp vasishavkp
      121 fisyhgghga glgglggglg gaglgglslg glgglgglgg lgglgglglk glkaw
//
LOCUS       XP_077525191             395 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136591 [Amblyomma americanum].
ACCESSION   XP_077525191
VERSION     XP_077525191.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077669065.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..395
                     /product="uncharacterized protein LOC144136591"
                     /calculated_mol_wt=35571
     CDS             1..395
                     /gene="LOC144136591"
                     /coded_by="XM_077669065.1:136..1323"
                     /db_xref="GeneID:144136591"
ORIGIN      
        1 mravvftvvl yvllgkasag algagvpghf vgggagplgg qgvgvglgpg qgaglaaalg
       61 ggfngglgag llgaggpiia apngqgvsvp apvatpiasv tflkqpvvnl ryvtkpviky
      121 vkhpvatvhh airpvvrvgq aaggislngh glagftsalg sgaggfngga aalnglnnvk
      181 aaaikaqaaa ansltgpvng kaggfeglgg hgllghglpg palvgpgigg pglggpgigg
      241 pglggpglgg pglggpglgg hglgghglgg pglggqglga lglhslamqg lgmhglggvg
      301 ghglvgpvfg glggpgivgl garhpalagi glggigyngg pafkaglgng gaggdggttl
      361 lgalgadgid gavaairtah nlspsvavks ggkpy
//
LOCUS       XP_077525202             157 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136599 [Amblyomma americanum].
ACCESSION   XP_077525202
VERSION     XP_077525202.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077669076.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..157
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..157
                     /product="uncharacterized protein LOC144136599"
                     /calculated_mol_wt=15682
     CDS             1..157
                     /gene="LOC144136599"
                     /coded_by="XM_077669076.1:67..540"
                     /db_xref="GeneID:144136599"
ORIGIN      
        1 mrtarreast rsflpitmrn tlfvvgvavl cvavqlgrcg dvgpggfgaa dlalggfgae
       61 qlgfgggsga gllglaapga plavplaasv sfvrqpfvrv gyvarpvvty lhhpvatvsh
      121 tirpyvsmsq vlvgsnvpla gvgpgagglk agygwkk
//
LOCUS       XP_077525213             179 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136608 [Amblyomma americanum].
ACCESSION   XP_077525213
VERSION     XP_077525213.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077669087.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..179
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..179
                     /product="uncharacterized protein LOC144136608"
                     /calculated_mol_wt=16563
     CDS             1..179
                     /gene="LOC144136608"
                     /coded_by="XM_077669087.1:130..669"
                     /db_xref="GeneID:144136608"
ORIGIN      
        1 mnvfvvlatl vacayaggyg gyggyggygg gygghglggg lgfiggggyg ggygggyghg
       61 havavpvktv syvkkpvvsv gyvtrpvvsy vkqpvttvsh tvqpvvtvsh avvsaggygg
      121 gfggglggyg ggfggygggy gggygggygg gyggygggyg gygggygval vggkggyhg
//
LOCUS       XP_077525226             338 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136613 [Amblyomma americanum].
ACCESSION   XP_077525226
VERSION     XP_077525226.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077669100.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..338
                     /product="uncharacterized protein LOC144136613"
                     /calculated_mol_wt=30638
     CDS             1..338
                     /gene="LOC144136613"
                     /coded_by="XM_077669100.1:77..1093"
                     /db_xref="GeneID:144136613"
ORIGIN      
        1 mhraarcght wiclfpltri pkmnalvlil aatvsqalag gyaggavggg gaagsavvag
       61 gagvvhgggg glglggggaa lvpahvaavp lgghgpvghv kvapvktvsy vkkpivsvsy
      121 vtkpvvsyvk qpvttvshtv qpvvtlshav vgdlgglgag vgagggflga gfggglgslg
      181 avdggvsvvs gyglgnyglg vygagtggay gglgygggyg ggygvglkgt vvatdvgalg
      241 gyggglgygg gvtyatglgy ggggygggyg ggygggyggg ygggygtslg vagygvkgdl
      301 gvgavgvglk ggyalgagyg gglagvgvgt gygggygw
//
LOCUS       XP_077525238             343 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136618 [Amblyomma americanum].
ACCESSION   XP_077525238
VERSION     XP_077525238.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077669112.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..343
                     /product="uncharacterized protein LOC144136618"
                     /calculated_mol_wt=36637
     Region          19..78
                     /region_name="CCP"
                     /note="Complement control protein (CCP) modules (aka short
                     consensus repeats SCRs or SUSHI repeats) have been
                     identified in several proteins of the complement system;
                     cd00033"
                     /db_xref="CDD:153056"
     Site            order(29,51)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     Region          90..208
                     /region_name="Sad1_UNC"
                     /note="Sad1 / UNC-like C-terminal; cl23730"
                     /db_xref="CDD:474037"
     CDS             1..343
                     /gene="LOC144136618"
                     /coded_by="XM_077669112.1:157..1188"
                     /db_xref="GeneID:144136618"
ORIGIN      
        1 mkavalllll lsaeqllscs apglprngvf perdkmprdf aagttvtyac segfqllgss
       61 rrtclddggw hprglpfclv dvsasktahs sqprddpkla ldgnrttcss tlshlspwfl
      121 vdlrrafpis vvkldlphlv satpmlirvg nsstsfensv csvfeaelvr ghslflpcts
      181 pesgrylsvh lggfgsltvc eiaaysefad teegspettt vlrhdaanep panrsgllgm
      241 rgltgvsiga fvllapiccc cwcqrctkcc ckkssgnqel rgadnavfvl serdiglgya
      301 sswkdlesdg tvptgasgvt arlrdvsfet ptrsfydvdl nsp
//
LOCUS       XP_077525271             251 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136636 [Amblyomma americanum].
ACCESSION   XP_077525271
VERSION     XP_077525271.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077669145.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..251
                     /product="uncharacterized protein LOC144136636"
                     /calculated_mol_wt=25942
     CDS             1..251
                     /gene="LOC144136636"
                     /coded_by="XM_077669145.1:60..815"
                     /db_xref="GeneID:144136636"
ORIGIN      
        1 maadnaaqqp aetsgpaapa gpaapagpag psedapdaaq astsgstsha gedaadaqpr
       61 cqrlartlid alkpaengra pfsgrrglrf gcpvtyarng rvllpisrpk qtaggavlsa
      121 lggggggepg pptrrlllrg lvavaaflaa vafllstmfl aqslrsersr qqhkaaiaap
      181 vpmhapvpyl pqpeqeqvtd aeavhekrhk rrhhgrhrrr rgdaesrpgm peagsrqlea
      241 teaedtedgs a
//
LOCUS       XP_077525283            1432 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC144136643
            [Amblyomma americanum].
ACCESSION   XP_077525283
VERSION     XP_077525283.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077669157.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1432
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC144136643"
                     /calculated_mol_wt=160940
     Region          450..1010
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1432
                     /gene="LOC144136643"
                     /coded_by="XM_077669157.1:72..4370"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:144136643"
ORIGIN      
        1 mpkpfprrlk sssfrlsemg dslallavwk eqttgidqnv sllqrfftti diplrarfhi
       61 ssvaqqpgth teeryyqavc kytfpdtgsr avptsalqav sarvtqqwcl lgrmmpfrgh
      121 lqwliperer gfwcrwmphe adiqlaslsf gtfaglclfa qshvfyskpg apgytlscaf
      181 khderllqvm lklvhkkgcs htcssyklqi pyhsifrvvl neaetyhdal giylqlvtvp
      241 llyrsaydvn eweastgdgv wydrvfqlgc pcwwvmrsyd iggssvvklt lrnklkarqa
      301 isrlcarckp ettfqyspml tkkvgaklre ikmrchskik pnlcfptcya fsavlqqghd
      361 gfaqmallac ekleefeell irllhtsgpa lelalyairs aieeheivdi isclpklfak
      421 fctpaaalsk tpagtclvrr civtpskvil lppqihcqnr ilrkfdpefs lrvsfrddnm
      481 qnlsyslmsg hcrhmvierv vartlrdglv agnrrfrlla tscsqlrdhg awlyatdakg
      541 yssemirywm gdfsgiasta kkmarmgqcf ssteesvhvp llsnsvrevp dilggkhpvt
      601 aehyifsdgi gmispgllke vtdkigltvs psaiqiryag ykgmlcvnpl lsgrqivlrk
      661 smrkfncvns ecidvikisa prpvflnrql itlleqlgvp srvffclqqr mvtlfteplv
      721 sdsvalqvle tyvgpalpfg qllrhgfslt rdpfvrsilf avyrtsmesl lsktriavpr
      781 nlgrnmfgvl detgtlkygq vfvqftslgs strqqngppv filsgtvlvt kcpclhpgdv
      841 rkftavnvpa lhhikdcvvf pangfrphsd emagsdldgd eyiviwdrnl ffpghnqepm
      901 iygqkvqqqr selslvdgma kfisdfiknd nvgvmsnahl amadklkdgv fskqclsiaa
      961 kistcldfak tgvaavlekh erpreypdfm akgcnkntyr snrvlghlyr fqrflesvvs
     1021 tsfnsqlvdg rsnikllefh gwmsyrsvve elraayesdm drilkqygik teaevvsgfi
     1081 idtssfnksh yeksnvevlv tkqyravads trkrffkkve eacylksafs teeqttillr
     1141 masacfmvty snaqktcssf pwifsdlifl vmasasrrem phrqgnllia cldsqlppas
     1201 sqlsaksial evvlkwavke dlllqdagvr caricrhcle tlfinsarae daiangegap
     1261 spgcyvtsff ryvgstsvvf pkcdscswss qthtltmaal rtysllaifc dlshlglpcd
     1321 arlhepvqei qegndvrika pratwvsspv tspgfrdmll hgqdevvsll avwsgvhevr
     1381 irrgadrggg grhivvssvg rdwqrwflee lllqpwlata vemrsleqfi rg
//
LOCUS       XP_077525296            1422 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC144136650
            [Amblyomma americanum].
ACCESSION   XP_077525296
VERSION     XP_077525296.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077669170.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1422
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC144136650"
                     /calculated_mol_wt=159862
     Region          450..1010
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1422
                     /gene="LOC144136650"
                     /coded_by="XM_077669170.1:72..4340"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:144136650"
ORIGIN      
        1 mpkpfprrlk sssfrlsemg dslallavwk eqttgidqnv sllqrfftti diplrarfhi
       61 ssvaqqpgth teeryyqavc kytfpdtgsr avptsalqav sarvtqqwcl lgrmmpfrgh
      121 lqwliperer gfwcrwmphe adiqlaslsf gtfaglclfa qshvfyskpg apgytlscaf
      181 khderllqvm lklvhkkgcs htcssyklqi pyhsifrvvl neaetyhdal giylqlvtvp
      241 llyrsaydvn eweastgdgv wydrvfqlgc pcwwvmrsyd iggssvvklt lrnklkarqa
      301 isrlcarckp ettfqyspml tkkvgaklre ikmrchskik pnlcfptcya fsavlqqghd
      361 gfaqmallac ekleefeell irllhtsgpa lelalyairs aieeheivdi isclpklfak
      421 fctpaaalsk tpagtclvrr civtpskvil lppqihcqnr ilrkfdpefs lrvsfrddnm
      481 qnlsyslmsg hcrhmvierv vartlrdglv agnrrfrlla tscsqlrdhg awlyatdakg
      541 yssemirywm gdfsgiasta kkmarmgqcf ssteesvhvp llsnsvrevp dilggkhpvt
      601 aehyifsdgi gmispgllke vtdkigltvs psaiqiryag ykgmlcvnpl lsgrqivlrk
      661 smrkfncvns ecidvikisa prpvflnrql itlleqlgvp srvffclqqr mvtlftealv
      721 sdsvalqvle tyvgpalpfg qllrhgfslt rdpfvrsilf avyrtsmesl lsktriavpr
      781 nlgrnmfgvl detgtlkygq vfvqftslgs strqqngppv filsgtvlvt kcpclhpgdv
      841 rkftavnvpa lhhikdcvvf pangfrphsd emagsdldgd eyiviwdrnl ffpghnqepm
      901 iygqkvqqqr selslvdgma kfisdfiknd nvgvmsnahl amadklkdgv fskqclsiaa
      961 kistcldfak tgvaavlekh erpreypdfm akgcnkntyr snrvlghlyr fqrflesvvs
     1021 tsfnsqlvdg rsnikllefh gwmsyrsvve elraayesdm drilkqygik teaevvsgfi
     1081 idtssfnksh yeksnvevlv tkqyravads trkrffkkve eacypesafs teeqttillr
     1141 masacfmvty snaqktcssf pwifsdlifl vmasasrrem phrqgnllia cldsqlppas
     1201 sqlsaksial evvlkwavke dlllqdagvr caricrhcle tlfinsarae daiangegap
     1261 spgcyvtsff ryvgstsvvf pkcdscswss qthtltmaal rtysllaifc dlchlglpcd
     1321 arlhepvqei qegndvrikv tspgfrdmll hgqdevvsll avwsgvhevr irrgadrggg
     1381 grhivvssvg rdwqrwflee lllqpwlata vemrsleqfi rg
//
LOCUS       XP_077525306            1403 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136658 [Amblyomma americanum].
ACCESSION   XP_077525306
VERSION     XP_077525306.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077669180.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1403
                     /product="uncharacterized protein LOC144136658"
                     /calculated_mol_wt=157822
     Region          432..992
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1403
                     /gene="LOC144136658"
                     /coded_by="XM_077669180.1:1..4212"
                     /db_xref="GeneID:144136658"
ORIGIN      
        1 mgdsldflav wkeqtrgidd yvsllhrfft tidiplrarf hissvanqpg thteeryyqa
       61 vckytfpdtg sravptsalq avsarvtqqw cllgrmmpfr ghlqwliper ehgfwcrwmp
      121 headiqlasl sfgtfaglcl faqshvfysk pgapgytlsc afkhderllq vmlklvhkns
      181 cshtcssykl qipyhsifrv vlneaethhd algiylqlvt vpllyrsayd vneweastgd
      241 gvwydrvfql gcpcwwvmrs ydiggssvvk ltfrnklkar qaisrlsarc kpettfqysp
      301 mltkkvgakl reikmrchsk ikpnlcfptc yafsavlqqg hdgfaqmall acetleefee
      361 llirllhtsg palelalyai rsaieeheiv diisclpklf akfctpaaal skapagtclv
      421 rrcivtpskv illppqihcq nrilrkfdpe fslrvsfrdd nmqnlsyslm sshcrhmvie
      481 rvvartlrdg lvagnrrfrl latscsqlrd hgawlyatda kgyssemirh wmgdfsgias
      541 tpkkmarmgq cfssteesvh vpllsnsvre vpdilggkhp vtaehyifsd gigmispgll
      601 kevtdkiglt vspsaiqiry agykgmlcvn pllsgrqivl rksmrkfncv nsecieviki
      661 saprpvflnr qlitlleqlg vpsrvffclq qrmvtlftea lvsdsvalqv letyvgpvlp
      721 fgqllrhgfs ltrdpfvrsi lfavyrtsme sllsktriav prnlgrnmfg vldetgtlky
      781 gqvfvqftsl gsstrqqngp pvfilsgtvl vtkcpclhpg dvrkftavnv palhhikdcv
      841 vfpangfrph sdemagsdld gdeyiviwdr nlffpghnqe pmiygqkvqq qrselslvdg
      901 makfisdfik ndnvgvmsna hlamadklkd gvfskqclsi aakistcldf aktgvaavle
      961 kherpreypd fmakgchknt yrsnrvlghl yrfqrflesv vstsfnsqlv dgrsniklle
     1021 fhgwmsyrsv veelraayes dmdrilkqyg ikteaevvsg findtssfnk snyeksnvev
     1081 lvtkqyraia dstrkrffkk veeacylesa fsteeqttil lrmasacfmv tysnaqktcs
     1141 sfpwifseli llvmasasrr empyrqgnll iacldsqlpp assqlsaksi alevvlkwav
     1201 kedlllqdag vrcaricrhc letlfinsar aedaiangeg apspgcyvts ffryvgsasv
     1261 vfpkcdscsw ssqthtltma alrtysllai fcdlchlglp cdarlhepvq eiqegndvri
     1321 kvtspgfrdm llhgqdevvs llavwsgvhe vrirrgadrg ggrdivvssv grdwqrwfle
     1381 elllqpwlan avekrsleqf irg
//
LOCUS       XP_077525318            1404 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC144136666
            [Amblyomma americanum].
ACCESSION   XP_077525318
VERSION     XP_077525318.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077669192.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1404
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC144136666"
                     /calculated_mol_wt=157961
     Region          432..992
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1404
                     /gene="LOC144136666"
                     /coded_by="XM_077669192.1:1..4215"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:144136666"
ORIGIN      
        1 mgdslefiav wkeqtrgind yvsllhrfft tidiplrarf hissvaqqpg thteeryyqa
       61 vckytfpdtg sravptsalq avsarvtqqw cllgrmmpfr ghlqwliper ehgfwcrwmp
      121 headiqlasv sfgtfaglcl laqshvfysk pgapgytlsc afkhderllq vmlklvhkkg
      181 cshtcssykl qipyhsifrv vlneaethhd aqgiylqlvt vpllyrsayd vneweastgd
      241 gvwydrvfql gcpcwwvmrs ydiggssvvk ltlrnklkar qaisrlsvrc kpettfqysp
      301 mltknvgakl reikmrchsk ikpnlcfptc yafsavlqqg hdgfaqmall acekleefee
      361 llirllhtsg palelalyai rsaieeheiv diisclpklf akfctpaaal skapsgtclv
      421 rrcivtpskv illppqihcq nrilrkfdpe fslrvsfrdd nmqnlsyslm sshcrhmvie
      481 rvvartlrdg lvagnrrfrl latpcsqlrd hgawlyatda kgyssemiry wmgdfsgits
      541 takkmarmgq cfssteesvh vpllsnsvre vpvilggkhp vtdehyifsd gigmispgll
      601 kevtdkiglt vspsaiqiry agykgmlcvn pllsgrqivl rksmrkfncv nsecieviki
      661 saprpvflnr qlitlleqlg vpsrvffclq qrmvtlftea lvsdsvalqv letyvgpalp
      721 fgqllrhgfs ltrdpfvrsi lfavyrtsme sllsktriav prnlgrnmfg vldetgtlky
      781 gqvfvqftsl gsstrqqngp pvfilsgtvl vtkcpclhpg dvrkftavnv palhhikdcv
      841 vfpangfrph sdemagsdld gdeyiviwdr nlffpghnqe pmiygqkvqq qrselslvdg
      901 mtkfisdfik ndnvgvmsna hlamadklkd gvfskqclsi aakistcldf aktgvaavle
      961 kherpreypd fmakgchknt yrsnrvlghl yrfqrflesv vstsfnsqlv dgrsniklle
     1021 fhgwmsyrsv veelraayes dmdrilkqyg ikteaevvsg fiidtssfnk shyeksnvev
     1081 lvtrqyrava dstrkrffkk veeacylesa fsteeqttil lrmasacfmv tysnaqktcs
     1141 sfpwifsdvi llvmasasrr empyrqgnll iacldsqlpp assqlsaksi alevvlkwav
     1201 kedlllqdag vrcaricrhc letlfvnsar aedaiangeg apspgcyvts ffryvgstsv
     1261 vfpkcdscsw ssqthtltma alrtysllai fcdlchlglp cdarlhepvq eiqegndvri
     1321 kvtspgfrdm llhgqdevvs llavwsgvne vrirrgadrg gggrhivvss vgrdwqrwfl
     1381 eelllqpwla tavekrsleq firg
//
LOCUS       XP_077525361             395 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136697 [Amblyomma americanum].
ACCESSION   XP_077525361
VERSION     XP_077525361.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077669235.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..395
                     /product="uncharacterized protein LOC144136697"
                     /calculated_mol_wt=41419
     Region          30..113
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          <113..223
                     /region_name="PLN02217"
                     /note="probable pectinesterase/pectinesterase inhibitor"
                     /db_xref="CDD:215130"
     CDS             1..395
                     /gene="LOC144136697"
                     /coded_by="XM_077669235.1:15..1202"
                     /db_xref="GeneID:144136697"
ORIGIN      
        1 mgsqqfclkw nnhqsnmlav fyqmlsneal vdvtlacegl slkahkmvls acspffqalf
       61 aenpckhpiv ilkdvryvdl raivefmyrg evvvsqedlm allktaetlk vrglvtsvdg
      121 aadsgpvgsp raeapplskr krsrrprrrs psdsnksdye dappatrikv pdspeiiddg
      181 smssdhmtdp htaspasras nvasstavps garhgssvqa sisqhtqdsi gddvgegdda
      241 dfdvepldvm eesattesvp vfpdvasssq algdesqqsh htgstsgnsa lvpaqpslps
      301 dipitsqvdi epsptsltpy ddktiapvaa aspaaassgd gastsmaatd asgvpaiagp
      361 sgyqqstpsh gtypvpdhps hlhlsfcvtf aprls
//
LOCUS       XP_077525373             154 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136705 [Amblyomma americanum].
ACCESSION   XP_077525373
VERSION     XP_077525373.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077669247.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..154
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..154
                     /product="uncharacterized protein LOC144136705"
                     /calculated_mol_wt=16581
     Region          21..54
                     /region_name="Hairy_orange"
                     /note="Hairy Orange; cl02638"
                     /db_xref="CDD:470640"
     CDS             1..154
                     /gene="LOC144136705"
                     /coded_by="XM_077669247.1:375..839"
                     /db_xref="GeneID:144136705"
ORIGIN      
        1 mptpgysgdd vggseldwii agfracaqet lrflieeehl pgtdpmvlgl frhlilqela
       61 mdvdsvlsqq aaegepampe ppdeapfgip saeeeaarqm ydqlrnsqee qqpsdlqshl
      121 pavsstavph vnsdhsagqk tpptaepnpr epdc
//
LOCUS       XP_077525383             154 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136705 [Amblyomma americanum].
ACCESSION   XP_077525383
VERSION     XP_077525383.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077669257.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..154
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..154
                     /product="uncharacterized protein LOC144136705"
                     /calculated_mol_wt=16581
     Region          21..54
                     /region_name="Hairy_orange"
                     /note="Hairy Orange; cl02638"
                     /db_xref="CDD:470640"
     CDS             1..154
                     /gene="LOC144136705"
                     /coded_by="XM_077669257.1:314..778"
                     /db_xref="GeneID:144136705"
ORIGIN      
        1 mptpgysgdd vggseldwii agfracaqet lrflieeehl pgtdpmvlgl frhlilqela
       61 mdvdsvlsqq aaegepampe ppdeapfgip saeeeaarqm ydqlrnsqee qqpsdlqshl
      121 pavsstavph vnsdhsagqk tpptaepnpr epdc
//
LOCUS       XP_077525396             154 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136714 [Amblyomma americanum].
ACCESSION   XP_077525396
VERSION     XP_077525396.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077669270.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..154
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..154
                     /product="uncharacterized protein LOC144136714"
                     /calculated_mol_wt=16719
     CDS             1..154
                     /gene="LOC144136714"
                     /coded_by="XM_077669270.1:145..609"
                     /db_xref="GeneID:144136714"
ORIGIN      
        1 mptpfysgde vggneqdwnn egfrarvqdt lrlltaleql pgtdpmlldv lrflilqelv
       61 mdlnsvlnqq aaegepampe padeapfsig saveeatrqm ydqlrnrqge eqpsdlqshl
      121 aavssaavph vnsdhsggqk tpptaepnpr epdc
//
LOCUS       XP_077525419             394 aa            linear   INV 25-NOV-2025
DEFINITION  tachykinin-like peptides receptor 86C [Amblyomma americanum].
ACCESSION   XP_077525419
VERSION     XP_077525419.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077669293.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..394
                     /product="tachykinin-like peptides receptor 86C"
                     /calculated_mol_wt=44548
     Region          32..322
                     /region_name="7tmA_TACR"
                     /note="neurokinin receptors (or tachykinin receptors),
                     member of the class A family of seven-transmembrane G
                     protein-coupled receptors; cd15390"
                     /db_xref="CDD:320512"
     Region          33..59
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320512"
     Region          66..92
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320512"
     Site            order(88,91..92,104..109,111..112,115,158,160..164,199,
                     202..204,206..208,210..211,267,270..271,273..274,277,
                     289..290,292..294,297,300..301)
                     /site_type="other"
                     /note="putative peptide ligand binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:320512"
     Region          104..134
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320512"
     Region          145..166
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320512"
     Region          199..228
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320512"
     Region          247..277
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320512"
     Region          290..315
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320512"
     CDS             1..394
                     /gene="LOC144136726"
                     /coded_by="XM_077669293.1:340..1524"
                     /db_xref="GeneID:144136726"
ORIGIN      
        1 maneslwpps pdapvvevgg erqwvlawwa qllyallfgs vvavavlgna lvvwavlahr
       61 smrtttncfl vnlsladalt asfnavfnlv ymleshwafg qpycvfsnfl anltlassaf
      121 tiaamsvdry faivrplcgr prrrravatl cavwagsall slptllysrt rsyrygdrsv
      181 rtvcmlvwpd grlqgsfady vynivflvlt yavpvatmvv tysrmswvlw gsrcigeftq
      241 hqqialrnkq kvvkmlltvv vlfavswlpy hayfiylfhh pgaayadciq hvylamywla
      301 mahttynpiv yycmnkrfkr yfrrflccct psiepedeqr yslarsrcsv asrtrslqrg
      361 haskervttt isnihvcrsa plsspptect gvhi
//
LOCUS       XP_077525434             238 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136730 [Amblyomma americanum].
ACCESSION   XP_077525434
VERSION     XP_077525434.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077669308.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..238
                     /product="uncharacterized protein LOC144136730"
                     /calculated_mol_wt=25773
     CDS             1..238
                     /gene="LOC144136730"
                     /coded_by="XM_077669308.1:80..796"
                     /db_xref="GeneID:144136730"
ORIGIN      
        1 miqgsaepll aepatladnv gpchgrgavi eawyyaqahg gtparnasvl lgtaavypsa
       61 mkphrpygle lqqqwnhtvm fqagqpwcdv ktnllaileq cevtapvngr agqrgkrvgh
      121 spsrslarrg rrlrpgrphr hrgegshlvl lapghaaarv hrlardpavd esgargclqv
      181 sqeavhlidl rlvlleagsr glrptidlda frsapevprv pdfveprlmw ymavafdg
//
LOCUS       XP_077525455             108 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136743 [Amblyomma americanum].
ACCESSION   XP_077525455
VERSION     XP_077525455.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077669329.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..108
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..108
                     /product="uncharacterized protein LOC144136743"
                     /calculated_mol_wt=11853
     CDS             1..108
                     /gene="LOC144136743"
                     /coded_by="XM_077669329.1:157..483"
                     /db_xref="GeneID:144136743"
ORIGIN      
        1 maaanlesgp wkkcfqgtvs tyaraianim psetlatnfv hwsrcysttc fihfcvqkaa
       61 nrlgmrrsai sclvqhricg vprqshtyqn daaasaftay gcdlqdeq
//
LOCUS       XP_077525475             200 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136755 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077525475
VERSION     XP_077525475.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077669349.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..200
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..200
                     /product="uncharacterized protein LOC144136755 isoform X1"
                     /calculated_mol_wt=21730
     CDS             1..200
                     /gene="LOC144136755"
                     /coded_by="XM_077669349.1:32..634"
                     /db_xref="GeneID:144136755"
ORIGIN      
        1 mfqgsaeplq avpatladnv gpchwrgvvl etsydarahg gtrarnasvl lrtaavcpsa
       61 mkphrpysle lqqqwnhtvm fqagqpgsev ktnhlamleq ceatapvngr agqrgkrvgh
      121 spsrslarrg rrlrprrpnr hrgegshlvl lasghaavrd rrlarnpavd esgargclqv
      181 seeaehlvdl rldledsaar
//
LOCUS       XP_077525484             140 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136755 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077525484
VERSION     XP_077525484.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077669358.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..140
                     /product="uncharacterized protein LOC144136755 isoform X2"
                     /calculated_mol_wt=15498
     CDS             1..140
                     /gene="LOC144136755"
                     /coded_by="XM_077669358.1:304..726"
                     /db_xref="GeneID:144136755"
ORIGIN      
        1 mkphrpysle lqqqwnhtvm fqagqpgsev ktnhlamleq ceatapvngr agqrgkrvgh
       61 spsrslarrg rrlrprrpnr hrgegshlvl lasghaavrd rrlarnpavd esgargclqv
      121 seeaehlvdl rldledsaar
//
LOCUS       XP_077525497             178 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136766 [Amblyomma americanum].
ACCESSION   XP_077525497
VERSION     XP_077525497.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077669371.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..178
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..178
                     /product="uncharacterized protein LOC144136766"
                     /calculated_mol_wt=19607
     CDS             1..178
                     /gene="LOC144136766"
                     /coded_by="XM_077669371.1:270..806"
                     /db_xref="GeneID:144136766"
ORIGIN      
        1 mkphrpysle lqqpwnhtvm fqagqpgsev ktnhlamleq ceatapvngr agqrgkrvgh
       61 spshrlarrg rrlrprrphr hrgegshlvl lasghaavrd hrlarntavd esgargclqv
      121 seeaehlvdl rldlleagsr gfcsaidlda frsapevpsv pafvvprllw ymavafdg
//
LOCUS       XP_077525524             204 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136777 [Amblyomma americanum].
ACCESSION   XP_077525524
VERSION     XP_077525524.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077669398.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..204
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..204
                     /product="uncharacterized protein LOC144136777"
                     /calculated_mol_wt=22617
     CDS             1..204
                     /gene="LOC144136777"
                     /coded_by="XM_077669398.1:290..904"
                     /db_xref="GeneID:144136777"
ORIGIN      
        1 mkphrpysle lqqqwnhtvm fqagqpgsev ktnhlamleq cevtapvngr agqrgkrvgh
       61 spsrrlarrg rrlrprrphr hrgegshlvl lasghaaard hrlprdpavd esgargclqv
      121 seeaehlvdl rldlleagsr gfcsaidlda frsapevpsv pafvvprllw ymavafdgsh
      181 arrtceslag wlqdaaqake lkrq
//
LOCUS       XP_077525535             170 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144136782 [Amblyomma americanum].
ACCESSION   XP_077525535
VERSION     XP_077525535.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077669409.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..170
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..170
                     /product="uncharacterized protein LOC144136782"
                     /calculated_mol_wt=18772
     CDS             1..170
                     /gene="LOC144136782"
                     /coded_by="XM_077669409.1:273..785"
                     /db_xref="GeneID:144136782"
ORIGIN      
        1 mkphrpcsle lqqqwnhtvm fpagqpgsev ktnhlamleq cevtapdngr dgqrgkrvgh
       61 spsrrlarrg rrlrprrphr rrgegshlvl lasghaaard hrlardaavd esgargclqv
      121 seeaehlvdl rldlleagsr glrsaidlnd fssaaevpsv pdfvvprllw
//
LOCUS       XP_077482931             303 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093433 [Amblyomma americanum].
ACCESSION   XP_077482931
VERSION     XP_077482931.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077626805.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..303
                     /product="uncharacterized protein LOC144093433"
                     /calculated_mol_wt=33545
     Region          114..166
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          196..>303
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..303
                     /gene="LOC144093433"
                     /coded_by="XM_077626805.1:340..1251"
                     /db_xref="GeneID:144093433"
ORIGIN      
        1 mtgseepcgv aapvpdvpps phqaeevdga geetgvyvqt dmgmsdigal eqecirlndc
       61 vyttrsekvq lemteeslrs sepkvvfytg iasfttllai fkmlepfvsh svnnslakfq
      121 efvlflmkik lnlqnadlaf rfnvsestvs rifdkwlhva hcrlksqvmw persalqrtm
      181 pqafydsfgs dvaviidcfe ikierpssyl prsetwstyk gsntakylig iapqgivtfi
      241 segwggrvsd khitehcgll dnllpgdvvl adrgfnvads vgfyrarlhl paytkgkkql
      301 saa
//
LOCUS       XP_077482957             111 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077482957
VERSION     XP_077482957.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077626831.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..111
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=12689
     Region          11..79
                     /region_name="ArgoN"
                     /note="N-terminal domain of argonaute; pfam16486"
                     /db_xref="CDD:465134"
     CDS             1..111
                     /gene="LOC144093445"
                     /coded_by="XM_077626831.1:374..709"
                     /db_xref="GeneID:144093445"
ORIGIN      
        1 msrsrcsrrh kdgrknlytc cqlnfrertf tvdveedqri qfiikiqsaa tvnldalhgv
       61 fqkrvktgpq evtqaidivw rhgssiklap vgrsffrppg pnehninimi i
//
LOCUS       XP_077482978             128 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077482978
VERSION     XP_077482978.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077626852.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..128
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..128
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=14862
     Region          37..109
                     /region_name="ArgoN"
                     /note="N-terminal domain of argonaute; pfam16486"
                     /db_xref="CDD:465134"
     CDS             1..128
                     /gene="LOC144093454"
                     /coded_by="XM_077626852.1:884..1270"
                     /db_xref="GeneID:144093454"
ORIGIN      
        1 mssassrrlp rrrsrcistk inriviellv kkyrqdlanc ipafegrknl ymryhlnfre
       61 rtftvnleed qriqfiiniq yaatmnldal hcvfqncvni vpqaidivlr hassiklapv
      121 grsffrpp
//
LOCUS       XP_077482987             128 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077482987
VERSION     XP_077482987.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077626861.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..128
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..128
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=14862
     Region          37..109
                     /region_name="ArgoN"
                     /note="N-terminal domain of argonaute; pfam16486"
                     /db_xref="CDD:465134"
     CDS             1..128
                     /gene="LOC144093454"
                     /coded_by="XM_077626861.1:857..1243"
                     /db_xref="GeneID:144093454"
ORIGIN      
        1 mssassrrlp rrrsrcistk inriviellv kkyrqdlanc ipafegrknl ymryhlnfre
       61 rtftvnleed qriqfiiniq yaatmnldal hcvfqncvni vpqaidivlr hassiklapv
      121 grsffrpp
//
LOCUS       XP_077482999             248 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093466 [Amblyomma americanum].
ACCESSION   XP_077482999
VERSION     XP_077482999.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077626873.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..248
                     /product="uncharacterized protein LOC144093466"
                     /calculated_mol_wt=26018
     Region          <39..>166
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     CDS             1..248
                     /gene="LOC144093466"
                     /coded_by="XM_077626873.1:61..807"
                     /db_xref="GeneID:144093466"
ORIGIN      
        1 mpgvlgqrna qrqeerrlsw wessgafgqr wqpshrscsa alqqgvaasq qpradsdsqt
       61 paaersaqpi aapstqptva hsaepigsrp sygaaaaaag aanvaasspr pasaaaavsg
      121 sqpaaaalpp sqpcappvav tehtpeagdr atpgddvkik elrrtvasqf prrsahghlg
      181 rpillyanhf nigiptgsvy hyvvgifset ametkpmhqh edqqdshrap skevparpgk
      241 lhpgfrrl
//
LOCUS       XP_077483011             248 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093471 [Amblyomma americanum].
ACCESSION   XP_077483011
VERSION     XP_077483011.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077626885.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..248
                     /product="uncharacterized protein LOC144093471"
                     /calculated_mol_wt=25957
     Region          <39..>156
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     CDS             1..248
                     /gene="LOC144093471"
                     /coded_by="XM_077626885.1:30..776"
                     /db_xref="GeneID:144093471"
ORIGIN      
        1 mpgvlgqrna qrqeerrlsw wessgafgqr wqpshrscsa alqqgvaasq qpradsdsqt
       61 paaersaqpi aapstqptva hsaepigsrp sygaaaaaag aanvaasspr pasaaaavsg
      121 sqpaaaalpp sqpcappvav tehtpeagdr dtpgddvkik elgrtvasqf prrpahghlg
      181 rpillyanhf nigiptgsvy hyvvgifset ametkpmhqh edqqdshrap skevparpgk
      241 lhpgfrrp
//
LOCUS       XP_077483024             248 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093474 [Amblyomma americanum].
ACCESSION   XP_077483024
VERSION     XP_077483024.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077626898.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..248
                     /product="uncharacterized protein LOC144093474"
                     /calculated_mol_wt=25957
     Region          <39..>156
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     CDS             1..248
                     /gene="LOC144093474"
                     /coded_by="XM_077626898.1:13..759"
                     /db_xref="GeneID:144093474"
ORIGIN      
        1 mpgvlgqrna qrqeerrlsw wessgafgqr wqpshrscsa alqqgvaasq qpradsdsqt
       61 paaersaqpi aapstqptva hsaepigsrp sygaaaaaag aanvaasspr pasaaaavsg
      121 sqpaaaalpp sqpcappvav tehtpeagdr dtpgddvkik elgrtvasqf prrpahghlg
      181 rpillyanhf nigiptgsvy hyvvgifset ametkpmhqh edqqdshrap skevparpgk
      241 lhpgfrrp
//
LOCUS       XP_077483038             153 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093478 [Amblyomma americanum].
ACCESSION   XP_077483038
VERSION     XP_077483038.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077626912.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..153
                     /product="uncharacterized protein LOC144093478"
                     /calculated_mol_wt=16497
     CDS             1..153
                     /gene="LOC144093478"
                     /coded_by="XM_077626912.1:61..522"
                     /db_xref="GeneID:144093478"
ORIGIN      
        1 mmnqpqgvdw eeeirpspsw dalyaeyerr atsrvvqptq qatsplpvge esssvrvqrw
       61 lfntpspdar rcfsvgpcig wplsriqpss drgepdapgl lpwhpagidg hgplaasrrr
      121 sysspaapmq aqiedgatnn avgtapisar pqa
//
LOCUS       XP_077483051             307 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093484 [Amblyomma americanum].
ACCESSION   XP_077483051
VERSION     XP_077483051.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077626925.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..307
                     /product="uncharacterized protein LOC144093484"
                     /calculated_mol_wt=33782
     Region          131..300
                     /region_name="PLN00062"
                     /note="TATA-box-binding protein; Provisional"
                     /db_xref="CDD:177693"
     CDS             1..307
                     /gene="LOC144093484"
                     /coded_by="XM_077626925.1:12..935"
                     /db_xref="GeneID:144093484"
ORIGIN      
        1 mvaraystsd taladvnsea ndveeehiqq larflhpfql qpqqddpvdq stltqqrnvs
       61 vmqpfpvsme vmpqslmqpq tpaghvhqss agmitgvrst skfasssmip stsglpmtpt
      121 tpaladpgif pqiqnvvstv nlgcrlnlka ialrawnvky npdhcralim rireprttas
      181 ifssgkmvca gakseeesrl aarkyariiq klgfeakfld frictivasc dvhfalrleg
      241 lvvthsefss yepqlypgli fkmkkprial lifpsgkvll tgakvtseiy eafnyiypvl
      301 kgvgihk
//
LOCUS       XP_077483071             163 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093498 [Amblyomma americanum].
ACCESSION   XP_077483071
VERSION     XP_077483071.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077626945.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..163
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..163
                     /product="uncharacterized protein LOC144093498"
                     /calculated_mol_wt=18473
     CDS             1..163
                     /gene="LOC144093498"
                     /coded_by="XM_077626945.1:132..623"
                     /db_xref="GeneID:144093498"
ORIGIN      
        1 mknqraafvh trrerqrrrt kslsqpktfv maiievsfge yvgtvtglfn fleivgglvl
       61 fglmcsiken thlniflfsv syafsfngih imicsffsrr tmeimrqvfy yvfyqmtaam
      121 syvgasvklv kdspavpvha vvgiitgglh flhfitvsyn vyl
//
LOCUS       XP_077483198             242 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093542 [Amblyomma americanum].
ACCESSION   XP_077483198
VERSION     XP_077483198.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627072.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..242
                     /product="uncharacterized protein LOC144093542"
                     /calculated_mol_wt=26505
     CDS             1..242
                     /gene="LOC144093542"
                     /coded_by="XM_077627072.1:78..806"
                     /db_xref="GeneID:144093542"
ORIGIN      
        1 mfqrsaelll eppatlgdna gpchsrgvvl ktwygarahg gtrarsasvl lgavavchsp
       61 mkphraysfe lqqpqwnhsf mlqawqprse rpdvktnlla ileqcevtep dhggagqrgk
      121 wvghspsrrm arrggrlrpr rsyrqrgegs hlvllapgna aarvrrlarh pavdesgvrg
      181 clqvseggeh lvdlrlvlfh agsrgphlat dldafrseal appmlgfvvq rvlwymagvf
      241 dg
//
LOCUS       XP_077483210             414 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093551 [Amblyomma americanum].
ACCESSION   XP_077483210
VERSION     XP_077483210.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627084.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..414
                     /product="uncharacterized protein LOC144093551"
                     /calculated_mol_wt=45814
     Region          63..154
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     CDS             1..414
                     /gene="LOC144093551"
                     /coded_by="XM_077627084.1:1..1245"
                     /db_xref="GeneID:144093551"
ORIGIN      
        1 mpnnaplapq ppaaplvcsg tprqrdpavf sglgdsdved wlasyervst hnrwddamkl
       61 nnvifyladv anlwfrnhea yiatwstfkt sftevfgrpg vrklraeqrl reraqqpget
      121 ftsyiedvvd lckrvnsdmt eadkiqnimk gidedafqmp lakqpatvas vislcqsyee
      181 lrkqrvltrr tvqpadvlsa dvetaggqql rhdiqkfire eiarqlalis gvrnpppvls
      241 ptlqqtiraq vaealpplpq qpvavpltya avlarppshp lspfqdatqa pppstpppdf
      301 syraqvaqps plftpavshy grlwrtrdnr picfacgfag hvarycrrrp sspadvlpps
      361 aygsyiqppr vqadplppaf stnrqaatsh rsssprrrsl spmsrrasps aaen
//
LOCUS       XP_077483223             182 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093561 [Amblyomma americanum].
ACCESSION   XP_077483223
VERSION     XP_077483223.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627097.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..182
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..182
                     /product="uncharacterized protein LOC144093561"
                     /calculated_mol_wt=20281
     CDS             1..182
                     /gene="LOC144093561"
                     /coded_by="XM_077627097.1:111..659"
                     /db_xref="GeneID:144093561"
ORIGIN      
        1 mkphraysfe lqqpqwnhsf mlqawqprse rpdvktnlla ileqcevtep dhggagqrgk
       61 rvghspsrrm arrggrlrpr rsyrqrgegs hlvllapsna aarvrrlarh pavdesgvrg
      121 clqvseggeh lvdlrlvlfh agsrgphlat dldafrseal appmlgfvvq rvlwymagvf
      181 dg
//
LOCUS       XP_077483234             150 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093569 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077483234
VERSION     XP_077483234.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627108.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..150
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..150
                     /product="uncharacterized protein LOC144093569 isoform X1"
                     /calculated_mol_wt=16561
     CDS             1..150
                     /gene="LOC144093569"
                     /coded_by="XM_077627108.1:71..523"
                     /db_xref="GeneID:144093569"
ORIGIN      
        1 mesplsppyp wertvmdldv afqqvttryv gsvhglfnfl eailgsiias lyfmfgvesr
       61 sqqflylsam tfgindvhfi fsallseads tflpssiyfv lyqltasfcy iinaivimqt
      121 ynspgdcifg gvgglicgil hvghliytlk
//
LOCUS       XP_077483242             135 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093569 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077483242
VERSION     XP_077483242.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627116.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..135
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..135
                     /product="uncharacterized protein LOC144093569 isoform X2"
                     /calculated_mol_wt=14790
     CDS             1..135
                     /gene="LOC144093569"
                     /coded_by="XM_077627116.1:185..592"
                     /db_xref="GeneID:144093569"
ORIGIN      
        1 mdldvafqqv ttryvgsvhg lfnfleailg siiaslyfmf gvesrsqqfl ylsamtfgin
       61 dvhfifsall seadstflps siyfvlyqlt asfcyiinai vimqtynspg dcifggvggl
      121 icgilhvghl iytlk
//
LOCUS       XP_077483249             135 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093569 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077483249
VERSION     XP_077483249.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627123.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..135
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..135
                     /product="uncharacterized protein LOC144093569 isoform X2"
                     /calculated_mol_wt=14790
     CDS             1..135
                     /gene="LOC144093569"
                     /coded_by="XM_077627123.1:215..622"
                     /db_xref="GeneID:144093569"
ORIGIN      
        1 mdldvafqqv ttryvgsvhg lfnfleailg siiaslyfmf gvesrsqqfl ylsamtfgin
       61 dvhfifsall seadstflps siyfvlyqlt asfcyiinai vimqtynspg dcifggvggl
      121 icgilhvghl iytlk
//
LOCUS       XP_077483262             436 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077483262
VERSION     XP_077483262.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627136.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..436
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=48729
     Region          1..436
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..436
                     /gene="LOC144093587"
                     /coded_by="XM_077627136.1:54..1364"
                     /db_xref="GeneID:144093587"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gtmdavrsgr fgplfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrdcqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqskdssy fvewipnnvk tavcdipprg
      361 lkmsatfign staiqelfkr iseqftamfr rkaflhwytg egmdelefte aesslnelvs
      421 eyqqyqeata edegef
//
LOCUS       XP_077483275             436 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077483275
VERSION     XP_077483275.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627149.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..436
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=48729
     Region          1..436
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..436
                     /gene="LOC144093593"
                     /coded_by="XM_077627149.1:54..1364"
                     /db_xref="GeneID:144093593"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gtmdavrsgr fgplfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrdcqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqskdssy fvewipnnvk tavcdipprg
      361 lkmsatfign staiqelfkr iseqftamfr rkaflhwytg egmdelefte aesslnelvs
      421 eyqqyqeata edegef
//
LOCUS       XP_077483299             180 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093609 [Amblyomma americanum].
ACCESSION   XP_077483299
VERSION     XP_077483299.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627173.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..180
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..180
                     /product="uncharacterized protein LOC144093609"
                     /calculated_mol_wt=18949
     CDS             1..180
                     /gene="LOC144093609"
                     /coded_by="XM_077627173.1:47..589"
                     /db_xref="GeneID:144093609"
ORIGIN      
        1 matapatsps hapapeplap agkaadaagg dagaqeggaa ddvpqlievn pryvttaqgt
       61 ltcletlgav avmvmyllms hsityqwyah lafllaftyc fnntliiisg vaspctqmhl
      121 petlfyslyh cmagllyvig gaifhnvhkh ddlllripag viltgvgvih cfhslvnfik
//
LOCUS       XP_077483313             112 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093616 [Amblyomma americanum].
ACCESSION   XP_077483313
VERSION     XP_077483313.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627187.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..112
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..112
                     /product="uncharacterized protein LOC144093616"
                     /calculated_mol_wt=11927
     CDS             1..112
                     /gene="LOC144093616"
                     /coded_by="XM_077627187.1:54..392"
                     /db_xref="GeneID:144093616"
ORIGIN      
        1 mepifasggv hmsanggals eatsatyaaa qsvnisswgn viaanasrar klarrddlaa
       61 akvvgiwlgc fliiflvvgi pnyiqkkmre aqensdrlfe rrlslghlrg pe
//
LOCUS       XP_077483325             657 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 1-like [Amblyomma americanum].
ACCESSION   XP_077483325
VERSION     XP_077483325.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627199.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..657
                     /product="endothelin-converting enzyme 1-like"
                     /calculated_mol_wt=74189
     Region          67..654
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..657
                     /gene="LOC144093623"
                     /coded_by="XM_077627199.1:94..2067"
                     /db_xref="GeneID:144093623"
ORIGIN      
        1 madakkhrtp ffccyllats aviavgtavc vtaiqillar sagpcttdac rhdrdrlasv
       61 vdpskapcdd fygfvcggat srpsggtvlr evemanlmrl ndsliayags knahafrklt
      121 tfyvscytay rekhdlratv hsflrnlgvs vkvwlraknl edlmdqvayl slhrnipsfl
      181 wirtepsglv flqpalpfsr rlnapvyedi qrllhdfvsv fdeitsqeer ifdlhvihvr
      241 lqavqiteeg lvvrvrqafs flshknmslw lsainkylpk nsqlapdslm airnadllgn
      301 ltrslqashw evrnlyvlmt lleavvewey trfrlvglsn aafetvyyct smsrslmtkl
      361 wtafvahtlv nreqsargir dvvsvvterf kddfaavawq getakartlq wlsvvrwnip
      421 derafdteva reslahvpem ggsyflnvgy lrlrasadav ddgdqyaaek pvvaydfnrn
      481 tltvtvgalv kplyysgael yinvvslgsl vayqlwnavv thkpwarqpr slylwvleqe
      541 agcyvrsyrn vseeagspda flklelysrv ralrtsldas sgsldmqikk papewpdvtq
      601 lqfffirycs mmcsrqffgl hhslfcdipv sstvqfkraf qcsrrdtlgs vdtcdvs
//
LOCUS       XP_077483397             205 aa            linear   INV 25-NOV-2025
DEFINITION  frequenin-2-like [Amblyomma americanum].
ACCESSION   XP_077483397
VERSION     XP_077483397.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627271.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..205
                     /product="frequenin-2-like"
                     /calculated_mol_wt=23930
     Region          7..133
                     /region_name="FRQ1"
                     /note="Ca2+-binding protein, EF-hand superfamily [Signal
                     transduction mechanisms]; COG5126"
                     /db_xref="CDD:444056"
     Region          99..>179
                     /region_name="FRQ1"
                     /note="Ca2+-binding protein, EF-hand superfamily [Signal
                     transduction mechanisms]; COG5126"
                     /db_xref="CDD:444056"
     CDS             1..205
                     /gene="LOC144093681"
                     /coded_by="XM_077627271.1:64..681"
                     /db_xref="GeneID:144093681"
ORIGIN      
        1 mgltkssqwr lhgdelielv ectglnpsil rrwhrefltd fprgfftree mkvvfgsmfp
       61 dghpnsfmdi vfnamdkrri gyvtfrdfvk tiaitvsgta ddklnwafdv fdqkgdgfvt
      121 ldeminvvea lwqlhgespe medykiqfir rrtrkifmrl dakkdgrisr dqfrevlkqd
      181 pwivknvlhv yssamgafsr vvhtn
//
LOCUS       XP_077483422             297 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093696 [Amblyomma americanum].
ACCESSION   XP_077483422
VERSION     XP_077483422.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627296.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..297
                     /product="uncharacterized protein LOC144093696"
                     /calculated_mol_wt=33047
     CDS             1..297
                     /gene="LOC144093696"
                     /coded_by="XM_077627296.1:86..979"
                     /db_xref="GeneID:144093696"
ORIGIN      
        1 maspvlasla lgllalraga idlesaarrp kfmecpkfdg hlvkcrdalm stpelndidl
       61 npdspehqaa ydmcllsklg vtmretmvwc ehkgslarkl alcyeyllrl kelpitdgli
      121 vkiidefqvc lekgegpvva lrerpskvsf gkippysdhs mrdedeyagy kerktmynsy
      181 aeednsgkga nsyqyakeks reygsdgesq tvdsmdnyed lgignspmts yrhrkrgepl
      241 gmdyanmpps yfyprkehgl gnettvdaak qataskpfgv kttthpakqe skparkp
//
LOCUS       XP_077483435             292 aa            linear   INV 25-NOV-2025
DEFINITION  carboxypeptidase B-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077483435
VERSION     XP_077483435.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627309.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..292
                     /product="carboxypeptidase B-like isoform X1"
                     /calculated_mol_wt=32380
     Region          1..284
                     /region_name="M14_CP_A-B_like"
                     /note="Peptidase M14 carboxypeptidase subfamily A/B-like;
                     cd03860"
                     /db_xref="CDD:349433"
     Site            order(47,50,105,122..123,175,227,229,250)
                     /site_type="active"
                     /db_xref="CDD:349433"
     CDS             1..292
                     /gene="LOC144093702"
                     /coded_by="XM_077627309.1:202..1080"
                     /db_xref="GeneID:144093702"
ORIGIN      
        1 mqtfslrrks lanvmnigks fenrdlkiik lssgrakrav wldggmhare wispatvmyi
       61 leelvsgysn dnettkildt fdvyglpvan pdgyeythif nrlwrktrst tasflcrgad
      121 pnrnfgfkws sggssskacs evfagnkafs epetkaianf iyarrreima yitfhsysql
      181 wltpwgytal kpanylelar aarvataale kvhgtkynvg tstsllyvas ggsddwalge
      241 aqipyaytve lrdtgrhgft lprdqivptg eetwaairal lleiaskaae ga
//
LOCUS       XP_077483441             235 aa            linear   INV 25-NOV-2025
DEFINITION  carboxypeptidase B-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077483441
VERSION     XP_077483441.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627315.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..235
                     /product="carboxypeptidase B-like isoform X2"
                     /calculated_mol_wt=25559
     Region          1..227
                     /region_name="Peptidase_M14_like"
                     /note="M14 family of metallocarboxypeptidases and related
                     proteins; cl11393"
                     /db_xref="CDD:472171"
     CDS             1..235
                     /gene="LOC144093702"
                     /coded_by="XM_077627315.1:202..909"
                     /db_xref="GeneID:144093702"
ORIGIN      
        1 mqtfslrrks lanvmnigks fenrdlkiik lssgrakrav wldggmhare wispatvmyi
       61 leeravpaar rarrslpgtr psrsrkprpl qtsstpgaar swptlpsipt hnsgsrrgvt
      121 rrssrpttss spcsdkrprt qaraarvata alekvhgtky nvgtstslly vasggsddwa
      181 lgeaqipyay tvelrdtgrh gftlprdqiv ptgeetwaai rallleiask aaega
//
LOCUS       XP_077483469             375 aa            linear   INV 25-NOV-2025
DEFINITION  lysosomal aspartic protease-like [Amblyomma americanum].
ACCESSION   XP_077483469
VERSION     XP_077483469.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627343.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..375
                     /product="lysosomal aspartic protease-like"
                     /calculated_mol_wt=40932
     Region          46..373
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(75,77,79,117..119,165)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133137"
     Site            75..77
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133137"
     Site            75
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133137"
     Site            order(117..120,124..127)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133137"
     CDS             1..375
                     /gene="LOC144093719"
                     /coded_by="XM_077627343.1:23..1150"
                     /db_xref="GeneID:144093719"
ORIGIN      
        1 msplgvtfll gllglsaaqf sislwrnrtd vdtrhrtavd aasvlpeele nennlhyygl
       61 itlgtpgqtf kvifdtgsan lwvpsiqcpn tedgckdkkk ydstksstyk kdgrtfkiey
      121 gsgvvqgfys tdvlgvgggs vpsqtfaeat kakgaifraa kfdgllglgy palaegnvvp
      181 vfdnmikqnl lpkpvfsvyl nrdpkatpgg eiyfgginsk hytggitytp vtkksywqfr
      241 mqgmqvkkdk nfcaggcdav idtgssfveg prdeierlns ylratetpag efkvkcsnip
      301 kfpkisftig gkeftmipdq yiikmettsk vkcfsafavs dtptkkfwil gqvfigsfys
      361 ifdrgsdrlg favaa
//
LOCUS       XP_077483485             153 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093720 [Amblyomma americanum].
ACCESSION   XP_077483485
VERSION     XP_077483485.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627359.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..153
                     /product="uncharacterized protein LOC144093720"
                     /calculated_mol_wt=17367
     CDS             1..153
                     /gene="LOC144093720"
                     /coded_by="XM_077627359.1:635..1096"
                     /db_xref="GeneID:144093720"
ORIGIN      
        1 mriltvavll caaaalasgr rryrirylda ddvddapvvf rrgrgrdllr dleamasgse
       61 rsgpvyvirr rkpkdrrlpg hssrervrfe eppedfdaee srrpgpplpg hdpfrptfvv
      121 qgtyalhprs qklmpyegqh pipiprevfs eis
//
LOCUS       XP_077483501             190 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093721 [Amblyomma americanum].
ACCESSION   XP_077483501
VERSION     XP_077483501.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627375.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..190
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..190
                     /product="uncharacterized protein LOC144093721"
                     /calculated_mol_wt=16096
     CDS             1..190
                     /gene="LOC144093721"
                     /coded_by="XM_077627375.1:1..573"
                     /db_xref="GeneID:144093721"
ORIGIN      
        1 mkstallcgl llvntvkagf lsgggggfgg ggyggggygd gglggafggd ggggyggggl
       61 gggggltstf lvrtvsktsg gggfggglgs yggglggygg ggygggghgg yssggygggl
      121 ggfggggggs ggaakviiik dgggeglggg fggglsgfgg lggssygggg yggggygggg
      181 yrgghhggwn
//
LOCUS       XP_077483514             117 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093724 [Amblyomma americanum].
ACCESSION   XP_077483514
VERSION     XP_077483514.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627388.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..117
                     /product="uncharacterized protein LOC144093724"
                     /calculated_mol_wt=11085
     CDS             1..117
                     /gene="LOC144093724"
                     /coded_by="XM_077627388.1:110..463"
                     /db_xref="GeneID:144093724"
ORIGIN      
        1 mrtlvvallv cclagvclag gyggyggygg gfglgygggf gggyghglgy tktvpgpsfl
       61 vktvhhvnrl shgahlvggh ygggygggyg ggyggfgggf gggyggfggy ggygwkg
//
LOCUS       XP_077483530             396 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093728 [Amblyomma americanum].
ACCESSION   XP_077483530
VERSION     XP_077483530.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627404.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..396
                     /product="uncharacterized protein LOC144093728"
                     /calculated_mol_wt=42618
     Region          <198..>238
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     CDS             1..396
                     /gene="LOC144093728"
                     /coded_by="XM_077627404.1:1..1191"
                     /db_xref="GeneID:144093728"
ORIGIN      
        1 mssvgavsaa qsgrgnrffe ataddyqvil pnlpsghivp ntvfmhgdpr arpyrvedyr
       61 dtlanlgalp dvvalgayqm nhvwavtmtt adaaqkllla vdvkvkdrpc llidpnnrev
      121 rvklhwllhg vtdedvrval spygkvlqvt rekwralgvt ekgsttrtvg lklhadvkid
      181 diphqlkiag eltlvvvpgr aplclrckst ghirrdckvp rcsrcrrfgh vdadcaktya
      241 svagpvqand isdhlmdeed seetartsrn lvtqgassnf qgeqlidggd kpadkrrhdd
      301 aeggnekdea sttghpspgg gekpptepap vtvmdsdsis aaakrphdag engkelsvva
      361 adeppakttv grrpsfrplp ntsaarktae tppmpp
//
LOCUS       XP_077483551             419 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093742 [Amblyomma americanum].
ACCESSION   XP_077483551
VERSION     XP_077483551.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627425.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..419
                     /product="uncharacterized protein LOC144093742"
                     /calculated_mol_wt=44782
     Region          <201..>235
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     Region          <237..>388
                     /region_name="PHA03377"
                     /note="EBNA-3C; Provisional"
                     /db_xref="CDD:177614"
     CDS             1..419
                     /gene="LOC144093742"
                     /coded_by="XM_077627425.1:17..1276"
                     /db_xref="GeneID:144093742"
ORIGIN      
        1 mssggaaiaa lagrgnrlva dadkdyeivl ptlptgrvvf ntvflhgdvr arpyrvedfr
       61 daladagalp evvalgayqi nhvwavtlss aeatkklaal kelqgkgrrc iifdpedqqv
      121 klrlhwmlhg vpdgdirtaf aafgnvtevt rerwrvqgmk ekgsttrtvl lklkpgmkvd
      181 dfphqlrvag elalvvvpgr pmqclrchgt ghvrrdckvp rcsqcrrygh tdadcvrtya
      241 satglgkand tvelmdvaea eeaatgtdea gkppstlatc vssedstkaa depasqpptv
      301 dcprfanets gngacptepe dveakepsed iheksgarat svtppvttpa krphdqtspe
      361 gdkvvapgve eppaktaqsr rpklrprpnl sddkraggkv lleqadvllp ddtggnsgv
//
LOCUS       XP_077483583             190 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093759 [Amblyomma americanum].
ACCESSION   XP_077483583
VERSION     XP_077483583.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627457.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..190
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..190
                     /product="uncharacterized protein LOC144093759"
                     /calculated_mol_wt=18705
     CDS             1..190
                     /gene="LOC144093759"
                     /coded_by="XM_077627457.1:126..698"
                     /db_xref="GeneID:144093759"
ORIGIN      
        1 mktivlvlaa laatmtsgvh ggydinalha lsavnaghgg ggggygggyg hgvpivkvgy
       61 vkkpyvtigy vpkkvvkfvk vpvktvgigi rpvltykvah qvkvghphfg yghggyghgy
      121 ghgygghglg kgisigfggh gigiglghgy ggggyghgfg hgigfghgfg hgygggggyg
      181 ggyggyhgfw
//
LOCUS       XP_077483597             102 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093767 [Amblyomma americanum].
ACCESSION   XP_077483597
VERSION     XP_077483597.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627471.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..102
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..102
                     /product="uncharacterized protein LOC144093767"
                     /calculated_mol_wt=9828
     CDS             1..102
                     /gene="LOC144093767"
                     /coded_by="XM_077627471.1:45..353"
                     /db_xref="GeneID:144093767"
ORIGIN      
        1 mhvywvillc alatgvaagg yghtsvsyva kpvlsvgyvs kpvvtyvkqp vatvshvvkp
       61 vltishgygg gygggggygg gggygggygg gggfgggygg wr
//
LOCUS       XP_077483639             172 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093805 [Amblyomma americanum].
ACCESSION   XP_077483639
VERSION     XP_077483639.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627513.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..172
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..172
                     /product="uncharacterized protein LOC144093805"
                     /calculated_mol_wt=19036
     Region          <93..>169
                     /region_name="TPR"
                     /note="TPR repeat [General function prediction only];
                     COG0790"
                     /db_xref="CDD:440553"
     Region          102..131
                     /region_name="SLR repeat"
                     /note="SLR repeat [structural motif]"
                     /db_xref="CDD:276807"
     CDS             1..172
                     /gene="LOC144093805"
                     /coded_by="XM_077627513.1:12..530"
                     /db_xref="GeneID:144093805"
ORIGIN      
        1 mtgarrrskq gkrnvakene gaeslpfggk vrlarrrkpd gwlvialevs atvavfllvy
       61 vgyyhreavh fhlnhayaha ghahaqhvva hkylkgsgvs knssmafywf rksadqghah
      121 saynlavghm qgfptdvqkg eakklikyaa dngvheahrv yheacrsgkc dh
//
LOCUS       XP_077483652             323 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093814 [Amblyomma americanum].
ACCESSION   XP_077483652
VERSION     XP_077483652.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627526.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..323
                     /product="uncharacterized protein LOC144093814"
                     /calculated_mol_wt=35645
     Region          222..322
                     /region_name="TGF_beta_BMPs_GDFs"
                     /note="transforming growth factor beta (TGF-beta) like
                     domain found in the BMP/GDF family; cd13756"
                     /db_xref="CDD:381634"
     Site            order(227,229,246,248..250,267..268,271..272,274..275,284,
                     287,289..290,293,313..315,317)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:381634"
     CDS             1..323
                     /gene="LOC144093814"
                     /coded_by="XM_077627526.1:59..1030"
                     /db_xref="GeneID:144093814"
ORIGIN      
        1 mmlsrsarrl lvtwpmnvil ttpivlltlg lsggeprnrt dipsvmmkly kknaakncdi
       61 rrivmsipan esiesggats ftfamlrgea aalaghtrla lefprkcgra tvvdatasgw
      121 tgsrqldfhr efssyvaqik gspvllqgga frvtlwtnkt hgfgcapsqa saslilnypn
      181 creqtqrrfk rhhfyesgtd sphpepdqvl wrgdqathsr gcrvvplrvh fkhigwdewi
      241 vapssymank cagecsssvl rdigganhat vlsllsgvrt ndvwhaccvp vgytrqtvly
      301 vdrhdnvvlr tftnmrvskc gcl
//
LOCUS       XP_077483662             442 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077483662
VERSION     XP_077483662.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627536.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..442
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=49513
     Region          1..442
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..442
                     /gene="LOC144093820"
                     /coded_by="XM_077627536.1:127..1455"
                     /db_xref="GeneID:144093820"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasgkyvp
       61 railvdlepg tmdavrsgqf gqlfrpdnfv fgqngagnnw akghytegae lvdsvldvvr
      121 keaescdclq gfqlthslgg gtgsgmgtll iskireeypd rimntysvvp spkvsdtvve
      181 pynatlsvhq lventdetyc idnealydic frtlklttpt ygdlnhlvsa tmsgvttclr
      241 fpgqlnadlr klavnmvpfp rlhffmtgfa pltsrgcqqy raltvpeltq qmfdaknmma
      301 acdprhgryl tvaavfrgrm smrevddqml niqnkqsshf vewipdnvkt avcdipprgl
      361 kmsatfigns taiqelfrri seqftamfrr kaflhwytge gmdeleftea esnlnelvse
      421 yqqyqeapde degefeetqa ea
//
LOCUS       XP_077483674             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077483674
VERSION     XP_077483674.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627548.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=49597
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144093828"
                     /coded_by="XM_077627548.1:103..1434"
                     /db_xref="GeneID:144093828"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gtmdavrsgq fgqlfrpdnf vfgqngagnn wakghytega elvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntynvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrgcqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkqssh fvewipdnvk tavcdipprg
      361 lkmsatfign staiqelfrr iseqftamfr rkaflhwytg egmdelefte aesnlnelvs
      421 eyqqyqeapd edegefeetq aea
//
LOCUS       XP_077483685             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077483685
VERSION     XP_077483685.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627559.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=49570
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144093835"
                     /coded_by="XM_077627559.1:103..1434"
                     /db_xref="GeneID:144093835"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gtmdavrsgq fgqlfrpdnf vfgqngagnn wakghytega elvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrgcqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkqssh fvewipdnvk tavcdipprg
      361 lkmsatfign staiqelfrr iseqftamfr rkaflhwytg egmdelefte aesnlnelvs
      421 eyqqyqeapd edegefeetq aea
//
LOCUS       XP_077483693             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077483693
VERSION     XP_077483693.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627567.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=49729
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144093843"
                     /coded_by="XM_077627567.1:130..1461"
                     /db_xref="GeneID:144093843"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gtmdavrsgq fgqlfrpdnf vfgqngagnn wakghytega elvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfprqlnadl rklavnmvpf prlhffmtgf apltsrgcqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkqssh fvewipdnvk tavcdipprg
      361 lkmsatfign staiqelfrr iseqftamfr rkaflhwytg egmdelefte aefnlnelvs
      421 eyqqyqeapd edegefeetq aea
//
LOCUS       XP_077483705             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077483705
VERSION     XP_077483705.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627579.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=49630
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144093850"
                     /coded_by="XM_077627579.1:105..1436"
                     /db_xref="GeneID:144093850"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gtmdavrsgq fgqlfrpdnf vfgqngagnn wakghytega elvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrgcqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkqssh fvewipdnvk tavcdipprg
      361 lkmsatfign staiqelfrr iseqftamfr rkaflhwytg egmdelefte aefnlnelvs
      421 eyqqyqeapd edegefeetq aea
//
LOCUS       XP_077483718             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077483718
VERSION     XP_077483718.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627592.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=49570
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144093858"
                     /coded_by="XM_077627592.1:120..1451"
                     /db_xref="GeneID:144093858"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gtmdavrsgq fgqlfrpdnf vfgqngagnn wakghytega elvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrgcqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkqssh fvewipdnvk tavcdipprg
      361 lkmsatfign staiqelfrr iseqftamfr rkaflhwytg egmdelefte aesnlnelvs
      421 eyqqyqeapd edegefeetq aea
//
LOCUS       XP_077483730             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077483730
VERSION     XP_077483730.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627604.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=49729
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144093866"
                     /coded_by="XM_077627604.1:153..1484"
                     /db_xref="GeneID:144093866"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gtmdavrsgq fgqlfrpdnf vfgqngagnn wakghytega elvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfprqlnadl rklavnmvpf prlhffmtgf apltsrgcqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkqssh fvewipdnvk tavcdipprg
      361 lkmsatfign staiqelfrr iseqftamfr rkaflhwytg egmdelefte aefnlnelvs
      421 eyqqyqeapd edegefeetq aea
//
LOCUS       XP_077483742             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077483742
VERSION     XP_077483742.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627616.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=49630
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144093873"
                     /coded_by="XM_077627616.1:102..1433"
                     /db_xref="GeneID:144093873"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gtmdavrsgq fgqlfrpdnf vfgqngagnn wakghytega elvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrgcqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkqssh fvewipdnvk tavcdipprg
      361 lkmsatfign staiqelfrr iseqftamfr rkaflhwytg egmdelefte aefnlnelvs
      421 eyqqyqeapd edegefeetq aea
//
LOCUS       XP_077483753             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077483753
VERSION     XP_077483753.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627627.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=49630
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144093882"
                     /coded_by="XM_077627627.1:1..1332"
                     /db_xref="GeneID:144093882"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gtmdavrsgq fgqlfrpdnf vfgqngagnn wakghytega elvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrgcqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkqssh fvewipdnvk tavcdipprg
      361 lkmsatfign staiqelfrr iseqftamfr rkaflhwytg egmdelefte aefnlnelvs
      421 eyqqyqeapd edegefeetq aea
//
LOCUS       XP_077483766             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077483766
VERSION     XP_077483766.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627640.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=49630
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144093890"
                     /coded_by="XM_077627640.1:102..1433"
                     /db_xref="GeneID:144093890"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gtmdavrsgq fgqlfrpdnf vfgqngagnn wakghytega elvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrgcqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkqssh fvewipdnvk tavcdipprg
      361 lkmsatfign staiqelfrr iseqftamfr rkaflhwytg egmdelefte aefnlnelvs
      421 eyqqyqeapd edegefeetq aea
//
LOCUS       XP_077483778             675 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093899 [Amblyomma americanum].
ACCESSION   XP_077483778
VERSION     XP_077483778.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627652.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..675
                     /product="uncharacterized protein LOC144093899"
                     /calculated_mol_wt=75694
     Region          84..351
                     /region_name="RT_nLTR_like"
                     /note="RT_nLTR: Non-LTR (long terminal repeat)
                     retrotransposon and non-LTR retrovirus reverse
                     transcriptase (RT). This subfamily contains both non-LTR
                     retrotransposons and non-LTR retrovirus RTs. RTs catalyze
                     the conversion of single-stranded RNA into...; cd01650"
                     /db_xref="CDD:238827"
     Site            order(173..178,279,281..282,347..348)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238827"
     Site            order(173..178,281)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238827"
     CDS             1..675
                     /gene="LOC144093899"
                     /coded_by="XM_077627652.1:46..2073"
                     /db_xref="GeneID:144093899"
ORIGIN      
        1 mpedklegss psapsqdqpd iawevtlpgm nralgrisac tatgpdgipa rllkllgpns
       61 reqlaelftt iinggdiped wrngrvvlll kpggnaahig dyraltvtsv vyrvfmqvlk
      121 awisgwaesa hvltelqngf rpgrrlednl fvltscteia rkegrrlicc fldvekaydn
      181 vphetlfhrl gtlgmappll etikrlyren vvtavfgnvh servyvhrgl rqgcplspll
      241 yllyvaglek allqanlgfg lkysttgidn ssrlpglafa ddlvimgent edvqkmldic
      301 aaelrslglk fnqrkcrvvq lageandqls ltlneetltv drsykylgin lcaetdmfrl
      361 heakvrqsal raqcilrrrc lwgchqytmv rdlwklahvp gltfanavvc lpsatrnwle
      421 rrqtevgrta vgchgrvana aiqgdlgwss feareacskl eyrgrlqfms hnrwarrvfe
      481 ylaatclrtt wvkrlhkieg kyglfqtpis aesatawkqe arrrvqeken rqwlqalaek
      541 ptlavyreck nsigpekffd nglgsallfe aragalrtlv yrrhfdatpe sqaaicracg
      601 daeetiehlv mscqrlhpmp tegttfpqal gfhgpavdde gdsskvkseg vlrtkrrltl
      661 wwstvfksak pqrrk
//
LOCUS       XP_077483852             205 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093950 [Amblyomma americanum].
ACCESSION   XP_077483852
VERSION     XP_077483852.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627726.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..205
                     /product="uncharacterized protein LOC144093950"
                     /calculated_mol_wt=23232
     Region          36..195
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..205
                     /gene="LOC144093950"
                     /coded_by="XM_077627726.1:247..864"
                     /db_xref="GeneID:144093950"
ORIGIN      
        1 myvqlgklpl waprsvinry mpavfqelyp strviidate ircevpsslv mqsgtysnyk
       61 santfkalvg vspddlltfa selfmgslsd reivirsgfl nqtfssgntv madkgfkiry
      121 lleekkvgln mpsflrqdqf ekedvqetqd iascrihver riqrikcyhi fdrpiplslg
      181 plinqiwsvc avlsnmqspl ilese
//
LOCUS       XP_077483862             232 aa            linear   INV 25-NOV-2025
DEFINITION  serine/threonine-protein phosphatase 4 regulatory subunit 1-like
            [Amblyomma americanum].
ACCESSION   XP_077483862
VERSION     XP_077483862.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627736.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..232
                     /product="serine/threonine-protein phosphatase 4
                     regulatory subunit 1-like"
                     /calculated_mol_wt=26681
     Site            order(16..17,20,23..24,57..58,61,64..65,68,96..97,100,
                     103..104,107,136..137,140,143..144,177..178,181)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          37..68
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          78..108
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          118..144
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          157..183
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..232
                     /gene="LOC144093956"
                     /coded_by="XM_077627736.1:17..715"
                     /db_xref="GeneID:144093956"
ORIGIN      
        1 mpsfegfvkd vdevrvglle rlalflrgls kadqrrllpl ladflcmdnq rswrfrfala
       61 rqlkeiaaly spldvhqylv pvvsellvdk vaevrhisve amvavlqqla pyylmrmevl
      121 qqlcekaqdr kwcqrqlfce laeqmisaec lsgeefigtv lpkllslrhd svpnvrmala
      181 rclvrgvwpk affwdygniy kdelkatlas lqedtdrdvr ffaqldpkgs ld
//
LOCUS       XP_077483884             281 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093970 [Amblyomma americanum].
ACCESSION   XP_077483884
VERSION     XP_077483884.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627758.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..281
                     /product="uncharacterized protein LOC144093970"
                     /calculated_mol_wt=30423
     Region          209..265
                     /region_name="bHLH_SOHLH_like"
                     /note="basic helix-loop-helix (bHLH) domain found in the
                     spermatogenesis- and oogenesis-specific basic
                     helix-loop-helix-containing protein (SOHLH) family and
                     similar proteins; cd19683"
                     /db_xref="CDD:381526"
     Site            order(209..214,217..218,220..221,243..244)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381526"
     Site            order(221,223..224,227..228,230..231,234,244..246,
                     248..249,251..252,254..256,258..259)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:381526"
     CDS             1..281
                     /gene="LOC144093970"
                     /coded_by="XM_077627758.1:69..914"
                     /db_xref="GeneID:144093970"
ORIGIN      
        1 mlwisrklqi ktlvsqrrqq rqrswqellv laaavynmaa aqstdlpcgv qncllhlddn
       61 edtadavnip ttdfesgncs ggdqqqqpqt hdtaeaattg qmqhtggyds lgyyhqsppm
      121 ysvyrvpphn sglpavggta anlgagpsts vaaaaagndg tlhaalggdv taaaagadva
      181 nsdimevarm asmgqmqhiv gpynvpfnrr edhnakerkr rsriknacqd lrslvpglte
      241 ktdkatvfef tvqyllhlrr hlgskrdksi sqevmekysp y
//
LOCUS       XP_077483894             792 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 1-like [Amblyomma americanum].
ACCESSION   XP_077483894
VERSION     XP_077483894.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627768.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..792
                     /product="endothelin-converting enzyme 1-like"
                     /calculated_mol_wt=89022
     Region          162..786
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..792
                     /gene="LOC144093977"
                     /coded_by="XM_077627768.1:99..2477"
                     /db_xref="GeneID:144093977"
ORIGIN      
        1 msdnspaade pqcqspvsee efsspgkses esetsspqse esvaaetaan tapsapedir
       61 vrkrlcnwva vcweaaifll afllgsvvit tfvwvatpda pslllalwsp vrpsrasqap
      121 tttarvdnsd aidadvpwgp casatcleqs rlllrqlnat ldpcddfyeh vcsnwvhahp
      181 lqpgqlgvsv ddlmlevytn tlvtaiqddr ggfanlrrff neclapeerl yyelmslfqk
      241 slglsgwntt tgskmaaaql sqvlgnlqrd laidivfrlt kvesasgngt llviqqpntv
      301 lvrskqsaqd eqflrqyfep llayfrrsfk tdvfvlekrl asffrrrddd nidrcptlqv
      361 sqlpvmqrie wlpllraafg detisksttv ilgapeyvfg lavedalpss kelihymlfr
      421 lsllllplss nsrvrdsfgs vgyatfpevh rplrqskacl rilnrfepni plflsrgfse
      481 svlggqqfih wllsrlrevf rdhvlkvqvf snalrgrlit gldaivwepl fpamllngsv
      541 rvqyaqeayq naspsasatv hqwltnssrr rlswlwrggf lsseptlvyp yrtleippat
      601 fslnvyndtt tgalqiaria priygrlfdv lhswtlaydq grrhqrkfvr sfasarscla
      661 hdydrmawqr kvprpgngsw pvqdlwdgla lplayeafqf ylrktdtslr istskgdlld
      721 aprlffvyya aslcenitpg lvrwnaanei kspawfrvng plrntpqfae afrcrskafm
      781 npprkcllss lg
//
LOCUS       XP_077483910             186 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144093981 [Amblyomma americanum].
ACCESSION   XP_077483910
VERSION     XP_077483910.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627784.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..186
                     /product="uncharacterized protein LOC144093981"
                     /calculated_mol_wt=20526
     CDS             1..186
                     /gene="LOC144093981"
                     /coded_by="XM_077627784.1:3..563"
                     /db_xref="GeneID:144093981"
ORIGIN      
        1 marhrstavv garrrassvr rtkpqqpkkd egppiippdv vlwaaaqsdy igfnpwfatg
       61 vvgmihvaet vlgftalall mfsptlglah rtllvtsfcm sytsllmllg smsspftdty
      121 mplslyfflf hlvaiifysg talytfwrsg edtfsklaav agafagyshy lhaqrcfvrv
      181 fnqped
//
LOCUS       XP_077483925             142 aa            linear   INV 25-NOV-2025
DEFINITION  adult-specific rigid cuticular protein 15.7-like [Amblyomma
            americanum].
ACCESSION   XP_077483925
VERSION     XP_077483925.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627799.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..142
                     /product="adult-specific rigid cuticular protein
                     15.7-like"
                     /calculated_mol_wt=14876
     Region          40..91
                     /region_name="Chitin_bind_4"
                     /note="Insect cuticle protein; pfam00379"
                     /db_xref="CDD:459790"
     CDS             1..142
                     /gene="LOC144093982"
                     /coded_by="XM_077627799.1:64..492"
                     /db_xref="GeneID:144093982"
ORIGIN      
        1 mtlapvaafl gllavvnagy hghhgghsnt yrkqndygey tfgydvvdgy gsihgrhesg
       61 saygpvvgsy ylgnidgrhr qvhyvadkwg framvktnep gtktslpaaa pyhsangkhv
      121 paygiggghg gyghggyggy hg
//
LOCUS       XP_077483937             148 aa            linear   INV 25-NOV-2025
DEFINITION  adult-specific rigid cuticular protein 15.7-like [Amblyomma
            americanum].
ACCESSION   XP_077483937
VERSION     XP_077483937.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627811.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="adult-specific rigid cuticular protein
                     15.7-like"
                     /calculated_mol_wt=15003
     Region          39..90
                     /region_name="Chitin_bind_4"
                     /note="Insect cuticle protein; pfam00379"
                     /db_xref="CDD:459790"
     CDS             1..148
                     /gene="LOC144093988"
                     /coded_by="XM_077627811.1:41..487"
                     /db_xref="GeneID:144093988"
ORIGIN      
        1 miakvatils laalaaagai apyggfsrth rkqddfgrys fgynivnglg atnsryetga
       61 aygpvvgsyt ladvdgrhrq vhyvadklgf ravvktnepg tktslaaaap fvsakgpvvp
      121 svgfgggyfp faggygggyg ggyggyyg
//
LOCUS       XP_077483949             143 aa            linear   INV 25-NOV-2025
DEFINITION  adult-specific rigid cuticular protein 15.7-like [Amblyomma
            americanum].
ACCESSION   XP_077483949
VERSION     XP_077483949.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627823.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..143
                     /product="adult-specific rigid cuticular protein
                     15.7-like"
                     /calculated_mol_wt=15047
     Region          40..91
                     /region_name="Chitin_bind_4"
                     /note="Insect cuticle protein; pfam00379"
                     /db_xref="CDD:459790"
     CDS             1..143
                     /gene="LOC144093996"
                     /coded_by="XM_077627823.1:43..474"
                     /db_xref="GeneID:144093996"
ORIGIN      
        1 mltqvvvflg vasvalggya gghhggssrt yrkqddyghy rfgydivngy gavngrheag
       61 naygpvhgsy ylgdidgrhr qvhyvadklg fravvktnep gtktslpaaa pyltahgkav
      121 payghggygg yggygygrhg yyg
//
LOCUS       XP_077483985             445 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094015 [Amblyomma americanum].
ACCESSION   XP_077483985
VERSION     XP_077483985.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627859.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..445
                     /product="uncharacterized protein LOC144094015"
                     /calculated_mol_wt=47060
     Region          <172..416
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..445
                     /gene="LOC144094015"
                     /coded_by="XM_077627859.1:106..1443"
                     /db_xref="GeneID:144094015"
ORIGIN      
        1 mqciraefqv aavtiamacm isityaasip ekafvpqhkr tdtsdsqlrl vkrtartflg
       61 fnsdgmlpfl rpqgnqgsrp rpvsleekrg nrsppkpprf qgfrpfpsif sdayiptplk
      121 peflenvlrp wnpspgegen ktpfwhgvyv vvpggapnmt grywcwsfvw pprrtkttkp
      181 pgskrpgaat qspgearpel pmptespaen prsevtepti pttlsdgdaa etrvpqsppe
      241 evtpssteft tvipragtap ttentrfsdv ptsvpattsg leveeaepnp trptisepav
      301 ttessstvss ppdeqgvqdg tppqgstmsa tgttgthstn adsagkdsps tdsagtdrps
      361 tnslrtdspn idtqgtgsag tdspgtdspg taipgtdtia psvdpslnev rptgsqipsg
      421 vldngrvgve trnytvkspg vikda
//
LOCUS       XP_077483998             173 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094024 [Amblyomma americanum].
ACCESSION   XP_077483998
VERSION     XP_077483998.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627872.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..173
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..173
                     /product="uncharacterized protein LOC144094024"
                     /calculated_mol_wt=17563
     CDS             1..173
                     /gene="LOC144094024"
                     /coded_by="XM_077627872.1:102..623"
                     /db_xref="GeneID:144094024"
ORIGIN      
        1 mlarckflfi agisfaahap madaspasia elkatmrthq elqtpagarv erqipvynrr
       61 lgfypygvrf gnpfasltag aafggvpfgg pfaggigsgl gagigagfgg slggflpirg
      121 passilvpql vvgyqrpflp yivgipypvy ipvlptagag ifpgdvglda vtn
//
LOCUS       XP_077484010             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094032 [Amblyomma americanum].
ACCESSION   XP_077484010
VERSION     XP_077484010.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627884.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144094032"
                     /calculated_mol_wt=21469
     Region          40..91
                     /region_name="Chitin_bind_4"
                     /note="Insect cuticle protein; pfam00379"
                     /db_xref="CDD:459790"
     CDS             1..217
                     /gene="LOC144094032"
                     /coded_by="XM_077627884.1:380..1033"
                     /db_xref="GeneID:144094032"
ORIGIN      
        1 miakfassvl claatvsagi iaasgghsvq hrkqddygny qfgydivngy gavngrheag
       61 gaygpvhgsy ylgdidgrhr qvhyvadklg fravvktnep gtktsapasa pyvsangkav
      121 paasvqaapv aivkpvipay gghlgyggsy gggygggygp vaaavvakaa vpvaayaaap
      181 hygygaigal ghgyglgygg yggyggyghg dghgylg
//
LOCUS       XP_077484024             136 aa            linear   INV 25-NOV-2025
DEFINITION  adult-specific rigid cuticular protein 15.7-like [Amblyomma
            americanum].
ACCESSION   XP_077484024
VERSION     XP_077484024.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627898.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..136
                     /product="adult-specific rigid cuticular protein
                     15.7-like"
                     /calculated_mol_wt=14082
     Region          39..90
                     /region_name="Chitin_bind_4"
                     /note="Insect cuticle protein; pfam00379"
                     /db_xref="CDD:459790"
     CDS             1..136
                     /gene="LOC144094040"
                     /coded_by="XM_077627898.1:125..535"
                     /db_xref="GeneID:144094040"
ORIGIN      
        1 migkfcilaa laavasaggy gghgghsvth rkqddygnyn fgynivngyg aangrhesgs
       61 ayggvkgayy lhdvdgrsrr veyvadkggf ramvhtnepg tktsypaaap yhsangkhvp
      121 ayghggygyg ghgyhg
//
LOCUS       XP_077484031             655 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094045 [Amblyomma americanum].
ACCESSION   XP_077484031
VERSION     XP_077484031.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627905.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..655
                     /product="uncharacterized protein LOC144094045"
                     /calculated_mol_wt=67704
     Region          24..112
                     /region_name="H15"
                     /note="linker histone 1 and histone 5 domains; the basic
                     subunit of chromatin is the nucleosome, consisting of an
                     octamer of core histones, two full turns of DNA, a linker
                     histone (H1 or H5) and a variable length of linker DNA;
                     H1/H5 are chromatin-associated...; cd00073"
                     /db_xref="CDD:238028"
     Site            order(27,39,71,85)
                     /site_type="DNA binding"
                     /note="DNA-binding site [nucleotide binding]"
                     /db_xref="CDD:238028"
     Region          <65..545
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Site            66..77
                     /site_type="DNA binding"
                     /note="DNA-binding site [nucleotide binding]"
                     /db_xref="CDD:238028"
     Site            101..112
                     /site_type="active"
                     /note="AKP helix motif (fragment) [active]"
                     /db_xref="CDD:238028"
     Region          <183..307
                     /region_name="TrbL"
                     /note="Type IV secretory pathway, TrbL components
                     [Intracellular trafficking, secretion, and vesicular
                     transport]; COG3846"
                     /db_xref="CDD:443056"
     CDS             1..655
                     /gene="LOC144094045"
                     /coded_by="XM_077627905.1:181..2148"
                     /db_xref="GeneID:144094045"
ORIGIN      
        1 mdsdsseeeq sstdqkaats rkapkmidki vdavnncadp kgaspafirn fifgkypdvy
       61 garfktsfkk afitaiekkv lvrtkateaa egvsgrvkla tkakrkeraa apaaqpesdr
      121 sknrrpstkt askkskssea vategrakst karkppglek kagenakpav adiksknttg
      181 kkaastgavv karkstgvaa kdekitgaaa kagrstgaaa ragrstgsta kagrstgsaa
      241 kagrstgiaa kagrssgaat kagrssgaat kagksrgaat kagksrgast kslkssgtav
      301 tvekgnaveg kadeenvveg kadeenaveg kedeanavev kvgkrrgvpa krvkdkafdd
      361 enkfldlels sengtsvaep rsegknsvae pesdgenssa eplsdgesss aepqsedess
      421 aaelqseent aaepqrdkgf aedavrlgdi gdsveqnmqg skgentatek raecgnssse
      481 sedtsgesde plspihmkke pgkkvaskkl mglkktaesn salqddfsgt geldssassa
      541 lkisklsvpp qdvgtesses edtsgesdep lntirmkkep gkkiaskklt glkktaesns
      601 arqddfsgtg epdssassak lsappqdgpt gdsvehdeep kakkqrpkpa rrakn
//
LOCUS       XP_077484051             656 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094056 [Amblyomma americanum].
ACCESSION   XP_077484051
VERSION     XP_077484051.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627925.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..656
                     /product="uncharacterized protein LOC144094056"
                     /calculated_mol_wt=67767
     Region          24..112
                     /region_name="H15"
                     /note="linker histone 1 and histone 5 domains; the basic
                     subunit of chromatin is the nucleosome, consisting of an
                     octamer of core histones, two full turns of DNA, a linker
                     histone (H1 or H5) and a variable length of linker DNA;
                     H1/H5 are chromatin-associated...; cd00073"
                     /db_xref="CDD:238028"
     Site            order(27,39,71,85)
                     /site_type="DNA binding"
                     /note="DNA-binding site [nucleotide binding]"
                     /db_xref="CDD:238028"
     Site            66..77
                     /site_type="DNA binding"
                     /note="DNA-binding site [nucleotide binding]"
                     /db_xref="CDD:238028"
     Region          <97..655
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Site            101..112
                     /site_type="active"
                     /note="AKP helix motif (fragment) [active]"
                     /db_xref="CDD:238028"
     Region          <196..296
                     /region_name="PRK13875"
                     /note="conjugal transfer protein TrbL; Provisional"
                     /db_xref="CDD:237537"
     CDS             1..656
                     /gene="LOC144094056"
                     /coded_by="XM_077627925.1:244..2214"
                     /db_xref="GeneID:144094056"
ORIGIN      
        1 mdsdsseeeq sstdqkaats rktpkmidki vdavnncadp kgasaafirn fifgkypdvy
       61 garfktsfkk afisaiekkv lvrtkateaa egvsgrvkla tkakrkeraa apaaqpesdr
      121 sknrrpstkt askkskssea vategrakst karkppglee kagenakpav adiksknttg
      181 kkaastgaea karkstgvaa kdekitgaaa kagrstgaaa ragrstgsta kagtspgaaa
      241 kagrstgaaa kagrstgaaa kagrssgaat kagksrgaat kagksrgaat kslkssgtav
      301 tvekgnaveg kadeenaveg kedeenaveg kedegnavev kvgkrrgvpa krvkdkafdd
      361 enkfldlels sengtsvaep rsegknsvae pesdgenssa eplsdgesss aepqsedess
      421 aaelqseeen taaepqrdkg faedavrlgd ignsveenmq gskgentate kraecgnsss
      481 esedtsgesd eplstihmkk epgkkiaskk lmglkktaes ntarqddfsg tgeldssass
      541 alkisklsvp pqdvgtesse sedtsgesde plntirmkke pgkkiaskkl tglkktaesn
      601 sarqddfsgt gepdssassa klsappqdgp tgdsvehdee pkakkqrpkp arrakn
//
LOCUS       XP_077484061             471 aa            linear   INV 25-NOV-2025
DEFINITION  endochitinase-like [Amblyomma americanum].
ACCESSION   XP_077484061
VERSION     XP_077484061.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627935.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..471
                     /product="endochitinase-like"
                     /calculated_mol_wt=53108
     Region          115..450
                     /region_name="GH18_chitinase-like"
                     /note="The GH18 (glycosyl hydrolase, family 18) type II
                     chitinases hydrolyze chitin, an abundant polymer of
                     beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a
                     major component of the cell wall of fungi and the
                     exoskeleton of arthropods. Chitinases have...; cl10447"
                     /db_xref="CDD:471972"
     CDS             1..471
                     /gene="LOC144094065"
                     /coded_by="XM_077627935.1:39..1454"
                     /db_xref="GeneID:144094065"
ORIGIN      
        1 mdysermrps lsrdapewae klpmvlskrr ipisifavig vltvagacfa avtavvlyrt
       61 avhelageeg aekpvalsgp fpgglpsrlr prsearrvfc vynnrlperq painftiedl
      121 kvqycddivy gfvgldgtat nirskhpkyd flddglkrfv glkrskpevn lwaclggssd
      181 dgprfatlvk errtrvtfvr nsaewlrrna fqglllywkh pgpehrvnfs vlmadlrnay
      241 ardnlltsvv lplntrlrrd syyvqslyen ldvvlvdghr svdprhfplt tcatpvrtll
      301 rawhqgqygl qralddlave tddfrktvls vsfsgvsftl rhrslhrvga aasgpgapga
      361 rtnesgllsy vevqerlrre gwkrgyhhyg rcpfarkdmq wvayedeesl ndkaglaemc
      421 sglavwdadm ddfaglvgkp cpllrkvhev vherashhkt psppksyrkf f
//
LOCUS       XP_077484083             117 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094073 [Amblyomma americanum].
ACCESSION   XP_077484083
VERSION     XP_077484083.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627957.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..117
                     /product="uncharacterized protein LOC144094073"
                     /calculated_mol_wt=12632
     CDS             1..117
                     /gene="LOC144094073"
                     /coded_by="XM_077627957.1:311..664"
                     /db_xref="GeneID:144094073"
ORIGIN      
        1 mkklpvgrgs rstmdnlply pyeplnrevr rllgnpgpsv kgffagqgev iekrpastpp
       61 sakpkeaspa esfeevevad kqtettaprt rgavsrplpc gphpsfssas tmhrhrk
//
LOCUS       XP_077484091             117 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094073 [Amblyomma americanum].
ACCESSION   XP_077484091
VERSION     XP_077484091.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627965.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..117
                     /product="uncharacterized protein LOC144094073"
                     /calculated_mol_wt=12632
     CDS             1..117
                     /gene="LOC144094073"
                     /coded_by="XM_077627965.1:249..602"
                     /db_xref="GeneID:144094073"
ORIGIN      
        1 mkklpvgrgs rstmdnlply pyeplnrevr rllgnpgpsv kgffagqgev iekrpastpp
       61 sakpkeaspa esfeevevad kqtettaprt rgavsrplpc gphpsfssas tmhrhrk
//
LOCUS       XP_077484104             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077484104
VERSION     XP_077484104.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627978.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=49505
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144094083"
                     /coded_by="XM_077627978.1:45..1376"
                     /db_xref="GeneID:144094083"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gtmdavrsgp fgplfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrgcqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkqssh fvewipdnvk tavcdipprg
      361 lkmsatfign staiqelfrr iseqftamfr rkaflhwytg egmdefefte aesnlnelvs
      421 eyqqyqeasd edegefeetq aea
//
LOCUS       XP_077484116             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077484116
VERSION     XP_077484116.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077627990.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=49505
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144094090"
                     /coded_by="XM_077627990.1:57..1388"
                     /db_xref="GeneID:144094090"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gtmdavrsgp fgplfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrgcqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkqssh fvewipdnvk tavcdipprg
      361 lkmsatfign staiqelfrr iseqftamfr rkaflhwytg egmdefefte aesnlnelvs
      421 eyqqyqeasd edegefeetq aea
//
LOCUS       XP_077484130             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077484130
VERSION     XP_077484130.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628004.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=49505
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144094098"
                     /coded_by="XM_077628004.1:57..1388"
                     /db_xref="GeneID:144094098"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gtmdavrsgp fgplfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrgcqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkqssh fvewipdnvk tavcdipprg
      361 lkmsatfign staiqelfrr iseqftamfr rkaflhwytg egmdefefte aesnlnelvs
      421 eyqqyqeasd edegefeetq aea
//
LOCUS       XP_077484151             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077484151
VERSION     XP_077484151.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628025.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=49486
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144094109"
                     /coded_by="XM_077628025.1:57..1388"
                     /db_xref="GeneID:144094109"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gtmdavrsgp fgplfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrgcqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkqssh fvewipdnvk tavcdipprg
      361 lkmsatfign staiqelfrr iseqftamfh rkaflhwytg egmdefefte aesnlnelvs
      421 eyqqyqeasd edegefeetq aea
//
LOCUS       XP_077484162             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077484162
VERSION     XP_077484162.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628036.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=49505
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144094116"
                     /coded_by="XM_077628036.1:57..1388"
                     /db_xref="GeneID:144094116"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gtmdavrsgp fgplfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrgcqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkqssh fvewipdnvk tavcdipprg
      361 lkmsatfign staiqelfrr iseqftamfr rkaflhwytg egmdefefte aesnlnelvs
      421 eyqqyqeasd edegefeetq aea
//
LOCUS       XP_077484170             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077484170
VERSION     XP_077484170.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628044.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=49477
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144094122"
                     /coded_by="XM_077628044.1:44..1375"
                     /db_xref="GeneID:144094122"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gtmdavrsgp fgplfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrgcqq yqaltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkqssh fvewipdnvk tavcdipprg
      361 lkmsatfign staiqelfrr iseqftamfr rkaflhwytg egmdefefte aesnlnelvs
      421 eyqqyqeasd edegefeetq aea
//
LOCUS       XP_077484183             763 aa            linear   INV 25-NOV-2025
DEFINITION  carboxy-terminal kinesin 2-like [Amblyomma americanum].
ACCESSION   XP_077484183
VERSION     XP_077484183.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628057.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..763
                     /product="carboxy-terminal kinesin 2-like"
                     /calculated_mol_wt=83262
     Region          <255..>427
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          423..756
                     /region_name="KISc_C_terminal"
                     /note="Kinesin motor domain, KIFC2/KIFC3/ncd-like
                     carboxy-terminal kinesins; cd01366"
                     /db_xref="CDD:276817"
     Site            order(433,510,513,515..518,660)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:276817"
     Site            order(707,710,713)
                     /site_type="other"
                     /note="microtubule interaction site [polypeptide binding]"
                     /db_xref="CDD:276817"
     CDS             1..763
                     /gene="LOC144094133"
                     /coded_by="XM_077628057.1:82..2373"
                     /db_xref="GeneID:144094133"
ORIGIN      
        1 msqppsqpsg sgqktaetgv rrrspsrrsi hgpgclrrsv stsaisedqp vntaeppkrt
       61 spgavmpkrr stckrsaaqg hgclrksvst tditedrpat taeppkrtrp gavvpkrhsl
      121 ckrsaaqghg cmrrsvsttd itedrpattl eppkrtrpga vvpkrrspik rttaqgrgnl
      181 rkslsttdvn vskpennaeq hkrprpgavk papsskekrn vpagcaasla tgvkssarpk
      241 appattalas saagkrklwd legrihdlev kikglpggke qlilqnttrq qdlerltadl
      301 saeriknsdl satvselqsa lctaeaklse alserdlltl rlasaeekva tlqrsyaetk
      361 galaelrcer talmaaqesl aadkerlesm vadlraklaa qeeqlhvseq drralhnslq
      421 elkgnirvfc rlrpllpted apkrpfltlp dertvqvlrt epkaqretvl tfsfdrvfpg
      481 aasqatvyae vselvqstld gynvcifayg qtgagkthsm egdeegehrg iipralhqvf
      541 easqkqphwt ytmvasfvev ynetlrdllv ppsqgdkapp lklkttskng litvvnltev
      601 pvksaqhigq llqcarknrv vaatkcners srshsvfrld itghnsytql dcrgrlnmvd
      661 lagserlses qvegvrlret qtinsslanl nnvivalsnr sshvpyrnsk lthllmdslg
      721 gnsktlmlln ispreenlme tinslrfatt vnkcqigias knp
//
LOCUS       XP_077484194             131 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094146 [Amblyomma americanum].
ACCESSION   XP_077484194
VERSION     XP_077484194.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628068.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..131
                     /product="uncharacterized protein LOC144094146"
                     /calculated_mol_wt=15029
     CDS             1..131
                     /gene="LOC144094146"
                     /coded_by="XM_077628068.1:617..1012"
                     /db_xref="GeneID:144094146"
ORIGIN      
        1 mrrppkgadp reiasdikdl vfllqehvvl vfknlqlhyd dphkvrfkdr rrrhlnrrlt
       61 atmkrlsgvh vlnhehrfld asgepmlslf aadryhvarr qgidpsahlf lcsgksrvkv
      121 atiilplqsa l
//
LOCUS       XP_077484225             693 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094178 [Amblyomma americanum].
ACCESSION   XP_077484225
VERSION     XP_077484225.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628099.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..693
                     /product="uncharacterized protein LOC144094178"
                     /calculated_mol_wt=76434
     CDS             1..693
                     /gene="LOC144094178"
                     /coded_by="XM_077628099.1:100..2181"
                     /db_xref="GeneID:144094178"
ORIGIN      
        1 mypasfsksp hrrfswatpa tvcgktqpeg gmdgpfease agwgstarsl asavpgpsff
       61 likeqpksll aikelqdrvh alegfrndif avqdiqqtmq sssnqpppvr llssikqpsk
      121 pvsecrgetr apsggaesls wtvnakprrh kggtltttvc grarakdagv gsgepgqgvv
      181 akpatvasvs skgdtasail etdkqdsgld shcrdhgsre stlapkdell slldiiderc
      241 vqlkerirqm ekasgwdsaq hgsrsevlef yekelqarln qievertkhr mvvrqlqati
      301 hrmegekvac eeklqvslse rkelekkvhs lhmqyvsgcp sslplakpea aeawpsmslh
      361 slgaqklage caqgitseeg kakvstifee cnpielkkql slytlenqal rekanedeqh
      421 wfaklsewka teaslrtdiq nliqqreeyl halkryqrgk pisqvkykel emtvwaldnp
      481 edqrayrrge dlppggnrfl rpadpgsmrs cnenfsckds sskaqslppm llnqakntpl
      541 isrrgayatd frrnslaens leeagfsieg madelvdrrl gaavmpseav rlmnleddgd
      601 kgakpgpqsn iemicnefdp lskaerqcas kpfdfedsld lsiplkptrv savnaskpsr
      661 ssvnmnmnyl tpgsgssrit ldqkhrtfgk nkn
//
LOCUS       XP_077484258             154 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094202 [Amblyomma americanum].
ACCESSION   XP_077484258
VERSION     XP_077484258.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628132.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..154
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..154
                     /product="uncharacterized protein LOC144094202"
                     /calculated_mol_wt=13147
     CDS             1..154
                     /gene="LOC144094202"
                     /coded_by="XM_077628132.1:179..643"
                     /db_xref="GeneID:144094202"
ORIGIN      
        1 mnalvftvvl salvgsavcg vlgggygglg glggglgggl alggaglggg ggvgvgssvv
       61 llggggagls kavagpsflv ntvhhvsqvh gggaivahsg lggvgggygl gglgagglgg
      121 lgggvllggg glggvglggg yggklllvkg gyhk
//
LOCUS       XP_077484266             113 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094210 [Amblyomma americanum].
ACCESSION   XP_077484266
VERSION     XP_077484266.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628140.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..113
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..113
                     /product="uncharacterized protein LOC144094210"
                     /calculated_mol_wt=10991
     CDS             1..113
                     /gene="LOC144094210"
                     /coded_by="XM_077628140.1:161..502"
                     /db_xref="GeneID:144094210"
ORIGIN      
        1 mkvlvlaval sclvvtvlag gfyhfggghg ggfgggyggg fgggyggghg gghggghggh
       61 gfgssglffv kgpggyghgf hgleavkgpt yfvsteqyvk kispgfvgkh yhh
//
LOCUS       XP_077484276             171 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094217 [Amblyomma americanum].
ACCESSION   XP_077484276
VERSION     XP_077484276.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628150.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..171
                     /product="uncharacterized protein LOC144094217"
                     /calculated_mol_wt=14952
     CDS             1..171
                     /gene="LOC144094217"
                     /coded_by="XM_077628150.1:60..575"
                     /db_xref="GeneID:144094217"
ORIGIN      
        1 mnslaillvg salavgamag vvaggglgyg gglgyggglg yggglgyggl gyggglgglg
       61 yggvgvggvg vgssvallsg gpgfskavag paflvrtvhh vnkvhgggai vahsglggvg
      121 gglgyggglg ygglgygggl gyggglgygg glgyggglgg ygvgvkyalk g
//
LOCUS       XP_077484287             176 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094222 [Amblyomma americanum].
ACCESSION   XP_077484287
VERSION     XP_077484287.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628161.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..176
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..176
                     /product="uncharacterized protein LOC144094222"
                     /calculated_mol_wt=15555
     CDS             1..176
                     /gene="LOC144094222"
                     /coded_by="XM_077628161.1:44..574"
                     /db_xref="GeneID:144094222"
ORIGIN      
        1 mnafvaslcl atvayvsagg lggfggyggy gggygggygg glgygglgyg gglgglgygg
       61 gvggvgvgss valirggpgf skavagpafv vrtvhhvnkv nsggaivahs giggvggglg
      121 yggglgyggg lgyggglgyg gglgyggglg yggglgyggg lgyggglkyg gyglkg
//
LOCUS       XP_077484298             149 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094234 [Amblyomma americanum].
ACCESSION   XP_077484298
VERSION     XP_077484298.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628172.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..149
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..149
                     /product="uncharacterized protein LOC144094234"
                     /calculated_mol_wt=13001
     CDS             1..149
                     /gene="LOC144094234"
                     /coded_by="XM_077628172.1:16..465"
                     /db_xref="GeneID:144094234"
ORIGIN      
        1 mamatdnvla gflgggyggg ygggfgggyg ggfgggfggg fgggvggvgv gssvvllnsg
       61 aggggkvvag paflvktvhh vnkihgggsl vahsgiggsg ggfgggfggg fgsglgggfg
      121 glygggyggg ygggygggfg ggygskgww
//
LOCUS       XP_077484309             138 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094245 [Amblyomma americanum].
ACCESSION   XP_077484309
VERSION     XP_077484309.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628183.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..138
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..138
                     /product="uncharacterized protein LOC144094245"
                     /calculated_mol_wt=12683
     CDS             1..138
                     /gene="LOC144094245"
                     /coded_by="XM_077628183.1:125..541"
                     /db_xref="GeneID:144094245"
ORIGIN      
        1 mnalaivaff aglvatafag glvgygglgy gglgygglgy gglgygglgy gglgvggvgv
       61 gssvallhgg ppfakavagp aflvrsvhhv nkihgggall ahsglgagyg glgygglgyg
      121 glgygglgyg glgygykg
//
LOCUS       XP_077484320             130 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094256 [Amblyomma americanum].
ACCESSION   XP_077484320
VERSION     XP_077484320.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628194.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..130
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..130
                     /product="uncharacterized protein LOC144094256"
                     /calculated_mol_wt=11942
     CDS             1..130
                     /gene="LOC144094256"
                     /coded_by="XM_077628194.1:113..505"
                     /db_xref="GeneID:144094256"
ORIGIN      
        1 mrtlvavfvv vaflstayag glgvgaglgy apvvgygggl gvggvgvgss vallnggpgf
       61 skavagpafl irtvhhvnki hgggaivahs glgaggvgyg glgygglgyg glgygglgyg
      121 glgygvgykg
//
LOCUS       XP_077484331              98 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094267 [Amblyomma americanum].
ACCESSION   XP_077484331
VERSION     XP_077484331.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628205.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..98
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..98
                     /product="uncharacterized protein LOC144094267"
                     /calculated_mol_wt=9611
     CDS             1..98
                     /gene="LOC144094267"
                     /coded_by="XM_077628205.1:34..330"
                     /db_xref="GeneID:144094267"
ORIGIN      
        1 mralvvaval callgvalag fygghgggfg ggyghgyhkt vpgpsflvkt vhhvhklhhg
       61 galfghahhg yggghggfgg ghgggvfllk ggyghgyh
//
LOCUS       XP_077484342              68 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4B chain-like [Amblyomma americanum].
ACCESSION   XP_077484342
VERSION     XP_077484342.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628216.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..68
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..68
                     /product="tubulin beta-4B chain-like"
                     /calculated_mol_wt=7474
     Region          1..>58
                     /region_name="PTZ00010"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:240228"
     CDS             1..68
                     /gene="LOC144094278"
                     /coded_by="XM_077628216.1:274..480"
                     /db_xref="GeneID:144094278"
ORIGIN      
        1 mreivhiqtg qcgnqigakf weaisdehgi dpsgayhgds dlqlerinvy yneasgtrla
       61 vfrliisl
//
LOCUS       XP_077484353             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077484353
VERSION     XP_077484353.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628227.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like isoform X1"
                     /calculated_mol_wt=49489
     Region          1..430
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144094289"
                     /coded_by="XM_077628227.1:89..1420"
                     /db_xref="GeneID:144094289"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gavdavrsgp ygplfrpdnl vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimniysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrgcqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkdssy fvewipnnvk tavcdipprg
      361 lkmsatfign staiqelfkr iseqftamfr rkaflhwytg egmdefefte aesnlnelvs
      421 eyqqyqeatp edeeefeetq aea
//
LOCUS       XP_077484361             442 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077484361
VERSION     XP_077484361.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628235.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..442
                     /product="tubulin beta-4 chain-like isoform X2"
                     /calculated_mol_wt=49432
     Region          1..429
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..442
                     /gene="LOC144094289"
                     /coded_by="XM_077628235.1:89..1417"
                     /db_xref="GeneID:144094289"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasgkyvp
       61 railvdlepg avdavrsgpy gplfrpdnlv fgqsgagnnw akghytegae lvdsvldvvr
      121 keaescdclq gfqlthslgg gtgsgmgtll iskireeypd rimniysvvp spkvsdtvve
      181 pynatlsvhq lventdetyc idnealydic frtlklttpt ygdlnhlvsa tmsgvttclr
      241 fpgqlnadlr klavnmvpfp rlhffmtgfa pltsrgcqqy raltvpeltq qmfdaknmma
      301 acdprhgryl tvaavfrgrm smrevddqml niqnkdssyf vewipnnvkt avcdipprgl
      361 kmsatfigns taiqelfkri seqftamfrr kaflhwytge gmdefeftea esnlnelvse
      421 yqqyqeatpe deeefeetqa ea
//
LOCUS       XP_077484372             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094307 [Amblyomma americanum].
ACCESSION   XP_077484372
VERSION     XP_077484372.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628246.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144094307"
                     /calculated_mol_wt=12866
     CDS             1..141
                     /gene="LOC144094307"
                     /coded_by="XM_077628246.1:105..530"
                     /db_xref="GeneID:144094307"
ORIGIN      
        1 mnslvialvv tcglvatvhc ggfgggghsv ivvkgiqagg gggagfghgs aasattagaa
       61 gpqvvqgpty vvktlhqvkk vshggaivgg gsgghsggyg gsfgfgggys hglsggfsgg
      121 yggfgkgygg lgghgghggw w
//
LOCUS       XP_077484382             100 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094318 [Amblyomma americanum].
ACCESSION   XP_077484382
VERSION     XP_077484382.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628256.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..100
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..100
                     /product="uncharacterized protein LOC144094318"
                     /calculated_mol_wt=9522
     CDS             1..100
                     /gene="LOC144094318"
                     /coded_by="XM_077628256.1:80..382"
                     /db_xref="GeneID:144094318"
ORIGIN      
        1 mnawvialvl cavtsvamag gyggghdaii ltghgggygg gygghsksvp gpsflvntvh
       61 hvhklshgga iiggydlvph ggghgggygg ghggygghgw
//
LOCUS       XP_077484395             481 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094323 [Amblyomma americanum].
ACCESSION   XP_077484395
VERSION     XP_077484395.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628269.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..481
                     /product="uncharacterized protein LOC144094323"
                     /calculated_mol_wt=52483
     Region          35..74
                     /region_name="RING-HC"
                     /note="HC subclass of RING (RING-HC) finger and its
                     variants; cd16449"
                     /db_xref="CDD:438113"
     CDS             1..481
                     /gene="LOC144094323"
                     /coded_by="XM_077628269.1:66..1511"
                     /db_xref="GeneID:144094323"
ORIGIN      
        1 magagrgaly rlrgfasgln wrptrfaddv sqyrtchmcc vlprrtvllp cqhslcepch
       61 gsrlkdgrva cpidgeafee sewhmvdvpa tkvenlkahc wnedrgcnfv gnlqavlqhf
      121 eeqcgfheva cprcgdsvln gdlppryepg cshgkplatp dqdswqgsaa alsdvsaelr
      181 eieeplkeps aahhvhegat alammvdlet ginelvqqtc tceetlnemg vaepgileap
      241 naevtqapgk ilvtlnearq aqqstcsaqq ercagggssa sggsnvdepp vqprqestnd
      301 lqklegmvsg avacleeilq carlqlalsg kpfvmveqvh ksvlkpspld rmerrrahfl
      361 ltvrnadklg mhgefgctvs aevkacrrqq scftvciasp glysplevaf issdsceaah
      421 lakamtvelr kcgyyynlpe rdglvpassg rlrlfyvdpk mchwwdplsl deltfdvtae
      481 e
//
LOCUS       XP_077484423             340 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094331 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077484423
VERSION     XP_077484423.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628297.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..340
                     /product="uncharacterized protein LOC144094331 isoform X1"
                     /calculated_mol_wt=36936
     CDS             1..340
                     /gene="LOC144094331"
                     /coded_by="XM_077628297.1:29..1051"
                     /db_xref="GeneID:144094331"
ORIGIN      
        1 mrrssssrsl rrassplpll sgpnaprrgr lrrttstpsl cfqswslsev prspttppts
       61 pfrqhdtaal lsdigeavda sgllsheqgf sdwdpdlpsr saqvelhaeg stqterlscp
      121 kdvellhsee sgfavlrflg vhgnksflvs lavlaavvft vavvatlsgt snankkinsl
      181 dlgqdgfese qasahkdamv taastarrmi tvhrmraark vissrkaaak grkkarishr
      241 nlrtrakehr trftttrpst rkqaksrrrp rtvptirrrg gkkkvsssrr spkgrrrlat
      301 gknrttttta ssgddvsten dlaqeseepk esssapslaa
//
LOCUS       XP_077484431             339 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094331 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077484431
VERSION     XP_077484431.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628305.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..339
                     /product="uncharacterized protein LOC144094331 isoform X2"
                     /calculated_mol_wt=36849
     CDS             1..339
                     /gene="LOC144094331"
                     /coded_by="XM_077628305.1:29..1048"
                     /db_xref="GeneID:144094331"
ORIGIN      
        1 mrrssssrsl rrassplpll sgpnaprrgr lrrttstpsl cfqswslsev prspttppts
       61 pfrqhdtaal lsdigeavda sgllsheqgf sdwdpdlpsr saqvelhaeg stqterlscp
      121 kdvellhsee sgfavlrflg vhgnksflvs lavlaavvft vavvatlsgt snankkinsl
      181 dlgqdgfese qasahkdamv taastarrmi tvhrmraark vissrkaaak grkkarishr
      241 nlrtrakehr trftttrpst rkqaksrrrp rtvptirrrg gkkkvsssrr spkgrrrlat
      301 gknrttttta sgddvstend laqeseepke sssapslaa
//
LOCUS       XP_077484442             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094340 [Amblyomma americanum].
ACCESSION   XP_077484442
VERSION     XP_077484442.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628316.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144094340"
                     /calculated_mol_wt=15840
     CDS             1..141
                     /gene="LOC144094340"
                     /coded_by="XM_077628316.1:89..514"
                     /db_xref="GeneID:144094340"
ORIGIN      
        1 mssisvssve eldspfprrf sqppellvds pvahtqlwrs ysepalwras wpvedrwppp
       61 alkhfhierl rfaaderpgd itwpvsrmeh yggpkttsse pnlsalcvwe sdnstetepn
      121 lsalslrqsd ksvetkpvdg e
//
LOCUS       XP_077484453             389 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094347 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077484453
VERSION     XP_077484453.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628327.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..389
                     /product="uncharacterized protein LOC144094347 isoform X1"
                     /calculated_mol_wt=41998
     CDS             1..389
                     /gene="LOC144094347"
                     /coded_by="XM_077628327.1:98..1267"
                     /db_xref="GeneID:144094347"
ORIGIN      
        1 metplsspgl rshrrasspp tlsvltptpv qprraastps fcflpcslse llwsarstpt
       61 ppprrcetea qvaatsrkqp kaadsvprlp fqsycarcwe pdvwsrstqt dlpadsstqt
      121 epvtllytgq lmqdedsrht frrlldercy mspffvtvaa lgvfvfilvt vstvfgtvnt
      181 gqkadnlell sdafeneapa vaaaarnnvq pitaptagka rgtavkletp kltaktrrpl
      241 rgasakahka trallrrpqk gvrgtnfkak ttptawrtgd asleanttst tlrteafstp
      301 tsttmastta ssttatarrp rprivgsrny vthnghlvev igdaltltsg aeessdedqq
      361 vateddvape rmrsqasptt htgfleeii
//
LOCUS       XP_077484461             388 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094347 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077484461
VERSION     XP_077484461.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628335.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..388
                     /product="uncharacterized protein LOC144094347 isoform X2"
                     /calculated_mol_wt=41911
     CDS             1..388
                     /gene="LOC144094347"
                     /coded_by="XM_077628335.1:98..1264"
                     /db_xref="GeneID:144094347"
ORIGIN      
        1 metplsspgl rshrrasspp tlsvltptpv qprraastps fcflpcslse llwsarstpt
       61 ppprrcetea qvaatsrkqp kaadsvprlp fqsycarcwe pdvwsrstqt dlpadsstqt
      121 epvtllytgq lmqdedsrht frrlldercy mspffvtvaa lgvfvfilvt vstvfgtvnt
      181 gqkadnlell sdafeneapa vaaaarnnvq pitaptagka rgtavkletp kltaktrrpl
      241 rgasakahka trallrrpqk gvrgtnfkak ttptawrtgd asleanttst tlrteafstp
      301 tsttmastta ssttatarrp rprivgsrny vthnghlvev igdaltltsg aeesdedqqv
      361 ateddvaper mrsqasptth tgfleeii
//
LOCUS       XP_077484466             387 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094347 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077484466
VERSION     XP_077484466.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628340.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..387
                     /product="uncharacterized protein LOC144094347 isoform X3"
                     /calculated_mol_wt=41813
     CDS             1..387
                     /gene="LOC144094347"
                     /coded_by="XM_077628340.1:98..1261"
                     /db_xref="GeneID:144094347"
ORIGIN      
        1 metplsspgl rshrrasspp tlsvltptpv qprraastps fcflpcslse llwsarstpt
       61 ppprrcetea qvaatsrkqp kaadsvprlp fqsycarcwe pdvwsrstqt dlpadsstqt
      121 epvtllytgq lmqdedsrht frrlldercy mspffvtvaa lgvfvfilvt vstvfgtvnt
      181 gqkadnlell sdafeneapa vaaaarnnvq pitaptaarg tavkletpkl taktrrplrg
      241 asakahkatr allrrpqkgv rgtnfkaktt ptawrtgdas leanttsttl rteafstpts
      301 ttmasttass ttatarrprp rivgsrnyvt hnghlvevig daltltsgae essdedqqva
      361 teddvaperm rsqaspttht gfleeii
//
LOCUS       XP_077484471             333 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094347 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077484471
VERSION     XP_077484471.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628345.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..333
                     /product="uncharacterized protein LOC144094347 isoform X4"
                     /calculated_mol_wt=35685
     CDS             1..333
                     /gene="LOC144094347"
                     /coded_by="XM_077628345.1:98..1099"
                     /db_xref="GeneID:144094347"
ORIGIN      
        1 metplsspgl rshrrasspp tlsvltptpv qprraastps fcflpcslse llwsarstpt
       61 ppprrcetea qvaatsrned srhtfrrlld ercymspffv tvaalgvfvf ilvtvstvfg
      121 tvntgqkadn lellsdafen eapavaaaar nnvqpitapt agkargtavk letpkltakt
      181 rrplrgasak ahkatrallr rpqkgvrgtn fkakttptaw rtgdaslean ttsttlrtea
      241 fstptsttma sttassttat arrprprivg srnyvthngh lvevigdalt ltsgaeessd
      301 edqqvatedd vapermrsqa sptthtgfle eii
//
LOCUS       XP_077484477             216 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094372 [Amblyomma americanum].
ACCESSION   XP_077484477
VERSION     XP_077484477.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628351.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..216
                     /product="uncharacterized protein LOC144094372"
                     /calculated_mol_wt=24808
     CDS             1..216
                     /gene="LOC144094372"
                     /coded_by="XM_077628351.1:78..728"
                     /db_xref="GeneID:144094372"
ORIGIN      
        1 mdwhgislrf ypagdeseli rekveqcvlm mvvgmvslgg slgvllmyia yaslndsgny
       61 iasmprsnas lsvrsmvwpw qkcpapkffk csakldswre pdaeswfflq trfgpgcfew
      121 dsgaqcpwrs pahfadlalc rrhchrvppa rrcleplvql ppdsecalpa pkdrraaarl
      181 wwfydplrhr crrwtdvcvf rafpsfedcf krclah
//
LOCUS       XP_077484481             532 aa            linear   INV 25-NOV-2025
DEFINITION  dorsal root ganglia homeobox [Amblyomma americanum].
ACCESSION   XP_077484481
VERSION     XP_077484481.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628355.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..532
                     /product="dorsal root ganglia homeobox"
                     /calculated_mol_wt=53232
     Region          49..105
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..532
                     /gene="Drgx"
                     /coded_by="XM_077628355.1:1..1599"
                     /db_xref="GeneID:144094382"
ORIGIN      
        1 mfcyhcpiss lgprppplpf svyspgspyg sygyqselhe dafvrrkqrr nrttftvqql
       61 eelekafaqt hypdvftred lamkinltea rvqvwfqnrr akwrkaerlr kereekdrsa
      121 agpdgaasgs aaaadagggg dlatgadngp pspvspgert dkqgvhssgs ltvhdeddde
      181 sqasaqsdsa gsaaaasstt ggvgamtsga sslldpsrla dqsrlealak aheamgspll
      241 aaaaaagrap lsllppplsa asralgvvas sassvmdhqq hhqhpffkda lssfaaaaaa
      301 slplrgmhqh plfsafaads slgghgggpp svgvgagsga gaggggaggc sapdsllgph
      361 prfplppmyf pphlsaqfaa ghaaaaaaaa asfpfkgmhp lcaccvaksh gtpphqapah
      421 aaapphhaas sdshhaaaal sqlgaaasaa aagasgggss aiitsgapal sgtagrnasg
      481 assgnggsde vtttssvsel rrkarehsqa vmlqsaamlk pppptatase sa
//
LOCUS       XP_077484492             410 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094403 [Amblyomma americanum].
ACCESSION   XP_077484492
VERSION     XP_077484492.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628366.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..410
                     /product="uncharacterized protein LOC144094403"
                     /calculated_mol_wt=42947
     Region          <149..382
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..410
                     /gene="LOC144094403"
                     /coded_by="XM_077628366.1:266..1498"
                     /db_xref="GeneID:144094403"
ORIGIN      
        1 mnanddkgkq askdmkqpsa kakesgkkrt kpskrsksks sssskasksg kkkdratkkr
       61 ngkardlaag psekkdaakk skprqekgas kkstsssgkr skssksskkg gkgrkrksvf
      121 rkiymklrgs sskskkskrk ksaksekapk lsqpatqpsg tsdarqpppd asassstlpe
      181 qapkesapes tdlgqktaeq ampdqkgpep kglgekasdl atveekpves gvpssvpadh
      241 akaspahage asvsdqvgks qdqkappvek edlgkvlqkd elvsatqgee kapgtpkepa
      301 qaehsgalks pppkplrlet qeatpghgqa aqpragavdg grqvaagasr preeviqper
      361 lpikdeerpe aaagtatfpc petdvehgga isvdwasfqa fllpefaavl
//
LOCUS       XP_077484496             424 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094414 [Amblyomma americanum].
ACCESSION   XP_077484496
VERSION     XP_077484496.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628370.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..424
                     /product="uncharacterized protein LOC144094414"
                     /calculated_mol_wt=46477
     Region          8..134
                     /region_name="MARVEL"
                     /note="Membrane-associating domain; pfam01284"
                     /db_xref="CDD:366555"
     CDS             1..424
                     /gene="LOC144094414"
                     /coded_by="XM_077628370.1:247..1521"
                     /db_xref="GeneID:144094414"
ORIGIN      
        1 mtyyftgfvr spggllkmiq iliglvlmli lmgghydvst glyrlridaf lmtmtvftfv
       61 ltsalllmct imgstelpns vlyrinylla tityfpftvg fifeeymhgd vkqtiaggvl
      121 sitnsliymf nlcvsyelrc lrgprrdrha kgrkskgkgk gkgkkgdldi lmdmdragga
      181 grpgrirner dllatgdvet fhrlqqiqrm nrllendpim fhqqasylmg aqqayldqqy
      241 aaqsggsaaa mtaqlhgggm ppadefaapr ddrlarrvvh evyrgeflpg eeylmqqpav
      301 gkeyiipiqv egkssqtsgt ttaeksetpk kdakspkgkk gkkspkkdkg gkkgskdkkk
      361 gskkgskkgs kskkkgskgk kggkkgskkg skkgskkgsk kgskskkkgs kgkkkkskgk
      421 kkkk
//
LOCUS       XP_077484500             594 aa            linear   INV 25-NOV-2025
DEFINITION  glutathione hydrolase 1 proenzyme-like [Amblyomma americanum].
ACCESSION   XP_077484500
VERSION     XP_077484500.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628374.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..594
                     /product="glutathione hydrolase 1 proenzyme-like"
                     /calculated_mol_wt=65221
     Region          69..584
                     /region_name="G_glu_transpept"
                     /note="Gamma-glutamyltranspeptidase; cl19223"
                     /db_xref="CDD:450276"
     CDS             1..594
                     /gene="LOC144094423"
                     /coded_by="XM_077628374.1:162..1946"
                     /db_xref="GeneID:144094423"
ORIGIN      
        1 mkhfwrrmil vwlllaslav alavglwffs yvenskvkrq prsispspmg vftswvaisg
       61 aehctntprr ilemngtiad vtvatmlcqc vtmpqkcglg ggsfavyynr anqsavafna
      121 rewapqaawt nmyianrtls ssgflacgvp gelmgyrrml dhigtnvpwa elfkdaerla
      181 rdgfpvspel ekmlkknepq iisddvlcnv fcshkrgenr stmtvnetlr npvlahtlai
      241 iaregsesfy ggsvgqmlvd diqrkggmis lpdlttysva vepalrrvlr dnvsvlappp
      301 paggiltefm iavmdsyrdp sapaenslvd ddttihrlie lskfafamrm emgdpnhidi
      361 taalrnlsss sflsevrski kgspysshsy yglryqgres kgssqfvvlm pngdalalms
      421 tlnkefgala msqstgvlln nqmddfatpg trnsygmlps ptnyirprkr ptssmspliv
      481 ahsdgnammv asasgafsic tglaqvvmra lwmnhtikea idaprvhhqm ypsavqaeks
      541 vdhdilhgll arghkveyfe wygqaiailr rpndthiyga ydsrallagg mdgd
//
LOCUS       XP_077484510             171 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094445 [Amblyomma americanum].
ACCESSION   XP_077484510
VERSION     XP_077484510.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628384.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..171
                     /product="uncharacterized protein LOC144094445"
                     /calculated_mol_wt=19406
     Region          <38..>88
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..171
                     /gene="LOC144094445"
                     /coded_by="XM_077628384.1:1..516"
                     /db_xref="GeneID:144094445"
ORIGIN      
        1 mmyskewile cnimrmkglq lyehmrrqni ltllskvtlq rylksyrtgf gfnenvlnil
       61 kqktstmdic krhgglnvde mklsenlcvs ssghiqgfvn mskftprsem hnvadhgmvi
      121 mfvpfagkwt qilacfatrg ntkgdlltki mleavilaen aglfvdfits d
//
LOCUS       XP_077484516             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094455 [Amblyomma americanum].
ACCESSION   XP_077484516
VERSION     XP_077484516.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628390.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144094455"
                     /calculated_mol_wt=14773
     CDS             1..133
                     /gene="LOC144094455"
                     /coded_by="XM_077628390.1:581..982"
                     /db_xref="GeneID:144094455"
ORIGIN      
        1 mqesvemped pahtgqeivf eyednlipvp atipctleeg ndstagsepa greqgtsger
       61 cgksnlrrkr psssllrelm amhqqaeera akaaqessel rkqlielqke snevqkgmlq
      121 vmkdylqske nke
//
LOCUS       XP_077484525             129 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094466 [Amblyomma americanum].
ACCESSION   XP_077484525
VERSION     XP_077484525.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628399.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..129
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..129
                     /product="uncharacterized protein LOC144094466"
                     /calculated_mol_wt=14583
     CDS             1..129
                     /gene="LOC144094466"
                     /coded_by="XM_077628399.1:1118..1507"
                     /db_xref="GeneID:144094466"
ORIGIN      
        1 mrlwraavrv avpderqlqr slekrnavnd sycrfrlptr negylqlvry tslyiyrrqg
       61 mthrtvyppg aggrvarfvp rppgllhagg akrgkicctd gavaqrqpvd trcgatlqrt
      121 srsedrqnl
//
LOCUS       XP_077484533             439 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase makorin-2-like [Amblyomma americanum].
ACCESSION   XP_077484533
VERSION     XP_077484533.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628407.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..439
                     /product="E3 ubiquitin-protein ligase makorin-2-like"
                     /calculated_mol_wt=49095
     Region          <6..>112
                     /region_name="YTH1"
                     /note="Cleavage and polyadenylation specificity factor
                     (CPSF) Clipper subunit and related makorin family
                     Zn-finger proteins [General function prediction only];
                     COG5084"
                     /db_xref="CDD:227416"
     Region          40..58
                     /region_name="zf_CCCH_4"
                     /note="Zinc finger domain; pfam18345"
                     /db_xref="CDD:465719"
     Region          <181..329
                     /region_name="PHA03096"
                     /note="p28-like protein; Provisional"
                     /db_xref="CDD:222981"
     Region          238..297
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(240,243,264,266,269,272,290,293)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          325..349
                     /region_name="ZnF_C3H1"
                     /note="zinc finger; smart00356"
                     /db_xref="CDD:214632"
     CDS             1..439
                     /gene="LOC144094476"
                     /coded_by="XM_077628407.1:44..1363"
                     /db_xref="GeneID:144094476"
ORIGIN      
        1 maeggsegvp cgyftdgvcr cgrqcpyahr ppsrqmdnvc ryylqgncmy gdrcrfehgw
       61 pgpragqpsg adslawgqgl rapnpgapag ggawrgarpa lleypsggry ppqaepptfs
      121 tsaawlgdpt setprasswa eapeaaggac wlpisstgsw ppeggvqdes lcpfevaneg
      181 cpigptcryl hrqvcdfcqe prlhpydqeq qmqhkeecvd qheremelaf amqrsagktc
      241 gicmdvvvdr eqpserrfgl lercshvfcl rcirhwrrsr vfdinvvrac pecrvpsdfv
      301 tpsnywvdtg eekdkliady ksamkekpcr yfrqgrgrcp fggacfykhe fpngspallp
      361 lprprrrqns dseameamea mgameameam enmenpedms lwvvvdvded plpelqwnev
      421 ytllmesgfd wadfeeqlg
//
LOCUS       XP_077484574             135 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094531 [Amblyomma americanum].
ACCESSION   XP_077484574
VERSION     XP_077484574.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628448.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..135
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..135
                     /product="uncharacterized protein LOC144094531"
                     /calculated_mol_wt=15159
     Region          21..129
                     /region_name="RNase_H2-C"
                     /note="Ribonuclease H2-C is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl07291"
                     /db_xref="CDD:415133"
     Site            order(23..27,46,52,54..59,77..80)
                     /site_type="other"
                     /note="heterotrimeric interface"
                     /db_xref="CDD:187752"
     CDS             1..135
                     /gene="LOC144094531"
                     /coded_by="XM_077628448.1:235..642"
                     /db_xref="GeneID:144094531"
ORIGIN      
        1 maasfkpael vlkmdkaedv dvhffacdin yrgkadlday fvvnvrpieg epghykgclr
       61 grplrgrrla lpdgysgiva rkaatessdt kelyvtcrfd nmiawnwssp mseekyrqmn
      121 ewiavaqalh apcde
//
LOCUS       XP_077484585             302 aa            linear   INV 25-NOV-2025
DEFINITION  CRISP/Allergen/PR-1-like [Amblyomma americanum].
ACCESSION   XP_077484585
VERSION     XP_077484585.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628459.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..302
                     /product="CRISP/Allergen/PR-1-like"
                     /calculated_mol_wt=34242
     Region          62..219
                     /region_name="CAP_euk"
                     /note="Eukaryotic CAP (cysteine-rich secretory proteins,
                     antigen 5, and pathogenesis-related 1 proteins) domain
                     proteins; cd05380"
                     /db_xref="CDD:349399"
     CDS             1..302
                     /gene="LOC144094539"
                     /coded_by="XM_077628459.1:187..1095"
                     /db_xref="GeneID:144094539"
ORIGIN      
        1 mwpstalwla asllagwpiv gqaqqpcrta ylktrsgvvh tacqprnrnc tilmrglssa
       61 dkkaflrrhn klrslvamgq lnrykpaanm yelvwddgla evaqaladqc gdiahdtada
      121 rftrrfrltg qnlaserrsn kvmkmdglrf idkwfneyrn yppyqvrnfe psfsdrhtgh
      181 ftqviwadtq ylgcgftmfr mkgdfgavpy qkhyvcnyah sgnikgrpvy tegptcsrcp
      241 agsrcvrrtg lcrarkksgr vhkpqkrpag rlsywivtrg tgagartwqq rrarctdagh
      301 kc
//
LOCUS       XP_077484592             302 aa            linear   INV 25-NOV-2025
DEFINITION  CRISP/Allergen/PR-1-like [Amblyomma americanum].
ACCESSION   XP_077484592
VERSION     XP_077484592.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628466.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..302
                     /product="CRISP/Allergen/PR-1-like"
                     /calculated_mol_wt=34242
     Region          62..219
                     /region_name="CAP_euk"
                     /note="Eukaryotic CAP (cysteine-rich secretory proteins,
                     antigen 5, and pathogenesis-related 1 proteins) domain
                     proteins; cd05380"
                     /db_xref="CDD:349399"
     CDS             1..302
                     /gene="LOC144094539"
                     /coded_by="XM_077628466.1:94..1002"
                     /db_xref="GeneID:144094539"
ORIGIN      
        1 mwpstalwla asllagwpiv gqaqqpcrta ylktrsgvvh tacqprnrnc tilmrglssa
       61 dkkaflrrhn klrslvamgq lnrykpaanm yelvwddgla evaqaladqc gdiahdtada
      121 rftrrfrltg qnlaserrsn kvmkmdglrf idkwfneyrn yppyqvrnfe psfsdrhtgh
      181 ftqviwadtq ylgcgftmfr mkgdfgavpy qkhyvcnyah sgnikgrpvy tegptcsrcp
      241 agsrcvrrtg lcrarkksgr vhkpqkrpag rlsywivtrg tgagartwqq rrarctdagh
      301 kc
//
LOCUS       XP_077484602             216 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094556 [Amblyomma americanum].
ACCESSION   XP_077484602
VERSION     XP_077484602.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628476.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..216
                     /product="uncharacterized protein LOC144094556"
                     /calculated_mol_wt=23540
     Region          <72..153
                     /region_name="RICIN"
                     /note="Ricin-type beta-trefoil; smart00458"
                     /db_xref="CDD:214672"
     CDS             1..216
                     /gene="LOC144094556"
                     /coded_by="XM_077628476.1:16..666"
                     /db_xref="GeneID:144094556"
ORIGIN      
        1 magisaesrn gvpasfrwpt lflalvvach lgtrasatfm gvqqveyaas iagaiksasd
       61 ilggvngegv fikslhgrgc ldsgcctidw tqrlqlnanc ptwgwlkwrs dgplivsqrp
      121 llrgclqcdd aepgcvalvs rctgrldqqw rlkfatnwtt efdeplfaia sffsperclt
      181 lvdgggdgdf yalrecatpr psewqsfyvv rnplkr
//
LOCUS       XP_077484614              66 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094566 [Amblyomma americanum].
ACCESSION   XP_077484614
VERSION     XP_077484614.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628488.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..66
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..66
                     /product="uncharacterized protein LOC144094566"
                     /calculated_mol_wt=7038
     CDS             1..66
                     /gene="LOC144094566"
                     /coded_by="XM_077628488.1:168..368"
                     /db_xref="GeneID:144094566"
ORIGIN      
        1 mkvvltilml vllativsag yyhhyghghg yghggyghgh yggyghghyg gyghyggygh
       61 ygygrr
//
LOCUS       XP_077484623             179 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094575 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077484623
VERSION     XP_077484623.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628497.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..179
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..179
                     /product="uncharacterized protein LOC144094575 isoform X1"
                     /calculated_mol_wt=19830
     Region          70..129
                     /region_name="CBM_14"
                     /note="Chitin binding Peritrophin-A domain; pfam01607"
                     /db_xref="CDD:426342"
     CDS             1..179
                     /gene="LOC144094575"
                     /coded_by="XM_077628497.1:80..619"
                     /db_xref="GeneID:144094575"
ORIGIN      
        1 mqvrlpgsal rcrtavhtna vmevfvsflc vsalvmyaav aetrvkrlay eladgadlvv
       61 gplvkafrcr rngyfadvrn rckvyhvcha vrraggssvm qhfsffcgnr tvfdqlsmtc
      121 typddavpcr sapaffylne rigvenvlfh tdtdvalada rrpkrrrtgd grtatrisg
//
LOCUS       XP_077484629             144 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094575 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077484629
VERSION     XP_077484629.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628503.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..144
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..144
                     /product="uncharacterized protein LOC144094575 isoform X2"
                     /calculated_mol_wt=16041
     Region          35..94
                     /region_name="CBM_14"
                     /note="Chitin binding Peritrophin-A domain; pfam01607"
                     /db_xref="CDD:426342"
     CDS             1..144
                     /gene="LOC144094575"
                     /coded_by="XM_077628503.1:97..531"
                     /db_xref="GeneID:144094575"
ORIGIN      
        1 myaavaetrv krlayeladg adlvvgplvk afrcrrngyf advrnrckvy hvchavrrag
       61 gssvmqhfsf fcgnrtvfdq lsmtctypdd avpcrsapaf fylnerigve nvlfhtdtdv
      121 aladarrpkr rrtgdgrtat risg
//
LOCUS       XP_077484638             450 aa            linear   INV 25-NOV-2025
DEFINITION  ornithine decarboxylase-like [Amblyomma americanum].
ACCESSION   XP_077484638
VERSION     XP_077484638.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628512.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..450
                     /product="ornithine decarboxylase-like"
                     /calculated_mol_wt=50093
     Region          30..392
                     /region_name="PLPDE_III_ODC"
                     /note="Type III Pyridoxal 5-phosphate (PLP)-Dependent
                     Enzyme Ornithine Decarboxylase; cd00622"
                     /db_xref="CDD:143482"
     Site            order(30,61,82..83,85..86,108,113,126,129,133,158..160,
                     281,283..284,299,301,303,306..307,315,317,341,343,345,348,
                     377,379,381..383,385)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143482"
     Site            order(59,61,80,144,187,190,226..227,264..267,316,344,373)
                     /site_type="active"
                     /db_xref="CDD:143482"
     Site            order(59,61,80,144,187,190,226..227,264..267,344,373)
                     /site_type="other"
                     /note="pyridoxal 5'-phosphate (PLP) binding site [chemical
                     binding]"
                     /db_xref="CDD:143482"
     Site            order(61,344)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:143482"
     Site            order(315..316,344..345,373,381)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:143482"
     CDS             1..450
                     /gene="LOC144094592"
                     /coded_by="XM_077628512.1:390..1742"
                     /db_xref="GeneID:144094592"
ORIGIN      
        1 msdasktgvy vedsvvnvat dviqqqgase afyvcdvrnl eykvqlwrhq lpnvtpfyav
       61 kcctdlvvlr tlnslgvnfd csnkteietv lelgvsperi vyanpiknvd hldfaeqnqv
      121 tlmtfdsvee ldkirdknar lllrihgnsv gcgitmnnkf gcsisdagkv lkkarcrnln
      181 iagvsfhiga afkhpavfqr tiedakevfd vgrelgfrmd tldigggfpg garklesfle
      241 lcrvireald ihfppssgid iiaepgqyfv tsaytlftkv igvrkrnvsv egkfcelqdv
      301 fineskcnti nrdlfdlmdv eislletpfn rprdtltviw gatchpwdiv vghshfyrvs
      361 vdewlmldnm gayslvsasg fngfgfprvh yiasaagapf vqlmveatrv rsgyghlhra
      421 leprragrpv afgvhtasiq hrdrecntfs
//
LOCUS       XP_077484645             450 aa            linear   INV 25-NOV-2025
DEFINITION  ornithine decarboxylase-like [Amblyomma americanum].
ACCESSION   XP_077484645
VERSION     XP_077484645.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628519.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..450
                     /product="ornithine decarboxylase-like"
                     /calculated_mol_wt=50093
     Region          30..392
                     /region_name="PLPDE_III_ODC"
                     /note="Type III Pyridoxal 5-phosphate (PLP)-Dependent
                     Enzyme Ornithine Decarboxylase; cd00622"
                     /db_xref="CDD:143482"
     Site            order(30,61,82..83,85..86,108,113,126,129,133,158..160,
                     281,283..284,299,301,303,306..307,315,317,341,343,345,348,
                     377,379,381..383,385)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143482"
     Site            order(59,61,80,144,187,190,226..227,264..267,316,344,373)
                     /site_type="active"
                     /db_xref="CDD:143482"
     Site            order(59,61,80,144,187,190,226..227,264..267,344,373)
                     /site_type="other"
                     /note="pyridoxal 5'-phosphate (PLP) binding site [chemical
                     binding]"
                     /db_xref="CDD:143482"
     Site            order(61,344)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:143482"
     Site            order(315..316,344..345,373,381)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:143482"
     CDS             1..450
                     /gene="LOC144094592"
                     /coded_by="XM_077628519.1:212..1564"
                     /db_xref="GeneID:144094592"
ORIGIN      
        1 msdasktgvy vedsvvnvat dviqqqgase afyvcdvrnl eykvqlwrhq lpnvtpfyav
       61 kcctdlvvlr tlnslgvnfd csnkteietv lelgvsperi vyanpiknvd hldfaeqnqv
      121 tlmtfdsvee ldkirdknar lllrihgnsv gcgitmnnkf gcsisdagkv lkkarcrnln
      181 iagvsfhiga afkhpavfqr tiedakevfd vgrelgfrmd tldigggfpg garklesfle
      241 lcrvireald ihfppssgid iiaepgqyfv tsaytlftkv igvrkrnvsv egkfcelqdv
      301 fineskcnti nrdlfdlmdv eislletpfn rprdtltviw gatchpwdiv vghshfyrvs
      361 vdewlmldnm gayslvsasg fngfgfprvh yiasaagapf vqlmveatrv rsgyghlhra
      421 leprragrpv afgvhtasiq hrdrecntfs
//
LOCUS       XP_077484667             390 aa            linear   INV 25-NOV-2025
DEFINITION  G-protein coupled receptor dmsr-1-like [Amblyomma americanum].
ACCESSION   XP_077484667
VERSION     XP_077484667.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628541.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..390
                     /product="G-protein coupled receptor dmsr-1-like"
                     /calculated_mol_wt=43513
     Region          61..361
                     /region_name="7tmA_FMRFamide_R-like"
                     /note="FMRFamide (Phe-Met-Arg-Phe) receptors and related
                     proteins, member of the class A family of
                     seven-transmembrane G protein-coupled receptors; cd14978"
                     /db_xref="CDD:410630"
     Region          61..87
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410630"
     Region          93..118
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410630"
     Site            order(115,118..119,135..140,142..143,146,191,193..197,230,
                     233..235,237..239,241..242,305,308..309,311..312,315,
                     328..329,331..333,336,339..340)
                     /site_type="other"
                     /note="putative ligand binding pocket [chemical binding]"
                     /db_xref="CDD:410630"
     Region          135..165
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410630"
     Region          177..197
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410630"
     Region          230..255
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410630"
     Region          286..316
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410630"
     Region          329..354
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410630"
     CDS             1..390
                     /gene="LOC144094619"
                     /coded_by="XM_077628541.1:60..1232"
                     /db_xref="GeneID:144094619"
ORIGIN      
        1 maeflnvsgm lqivdspyrd eenlsrtean lsgphgiter eelpyhgael grfdawytsh
       61 lhgylsvpvc vfgilanvln iivltqrnmv sptnriltgl avadmlviat ylpytiithv
      121 mpqaptqsce ravfvlvhsh vsvvfhtvst wltvtlavwr flavsfpass kkwcsmqrar
      181 wavvsmyvsc alcclplyls levhqigtpq epayrvnfsn ialandaflr nldfwaysvl
      241 mklvpcvalt glslgllrvl ykakarerrl rqgdcghtgh ggnseedrdr ttrmllavll
      301 lflvtefpsg ildllsgirg qdfvnhvynn lgavrdiltl vnstvnfily cfmsrqfrkt
      361 faalftpriv anwfpltsea pnsayattcv
//
LOCUS       XP_077484686             303 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094634 [Amblyomma americanum].
ACCESSION   XP_077484686
VERSION     XP_077484686.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628560.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..303
                     /product="uncharacterized protein LOC144094634"
                     /calculated_mol_wt=34145
     Region          135..184
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(136,139,154,161,164,167,180,183)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          <206..271
                     /region_name="TSPO_MBR"
                     /note="Translocator protein (TSPO)/peripheral-type
                     benzodiazepine receptor (MBR) family; cl01379"
                     /db_xref="CDD:470176"
     CDS             1..303
                     /gene="LOC144094634"
                     /coded_by="XM_077628560.1:144..1055"
                     /db_xref="GeneID:144094634"
ORIGIN      
        1 marrsqsssv qrmaegipie egpqtasres vrpqhtflap sndsqfcssa paqqhsrasa
       61 eipsilvhrt sdpfpgaddp vstfpsgvvm lhvpskvsiv aaskpgsspn rlrksssqrs
      121 vqsqkscssl tdhlvcrycf rddhpedmva pcrcikrstv rwthvrclek ymnrvhsnrc
      181 nmckkrmaai vrqksvlswf rhpstrrhql elvlavmlsl tmlpvlymaw ayafftvapq
      241 vnwliaivlt pylifqtstw lgfaaysfwi yydgmkswle ahavykfnhe ssvprggegy
      301 ssr
//
LOCUS       XP_077484695             667 aa            linear   INV 25-NOV-2025
DEFINITION  neprilysin-like [Amblyomma americanum].
ACCESSION   XP_077484695
VERSION     XP_077484695.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628569.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..667
                     /product="neprilysin-like"
                     /calculated_mol_wt=74113
     Region          44..665
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..667
                     /gene="LOC144094643"
                     /coded_by="XM_077628569.1:64..2067"
                     /db_xref="GeneID:144094643"
ORIGIN      
        1 mtivtiirsl pqesvttkat avkcisdeci rnlqhldqim hfgvdpcltf hsyvcgrfts
       61 ptkymlakmn hnlatkayes aldakvpatp lsaedkahkl fhmcldlhsa krkqpealvk
      121 lldelklnpa sikdgegedp ldrmmglsft ygvhalvefv adsvvivkkk rllrvsvase
      181 eflwheerkn lveqnelaay ylsrfvaigv fsseegakey vpkltelett aiihlaesvg
      241 skalgtesva vktiakhteg qvdadkwlsl lakhgaygge dravvqpavl kflarllgqs
      301 gaegvrrlls wslvrqwapl vdgsldkqgp larnvscvda vlavlrlpfl ahvlfdsisk
      361 erlrdattia tkmrdmlssl vanlsvfnqd skkkvmnkle rlkitvgfpd svdttqkvda
      421 lyaklalskt tffenwleas kykmiiasad skaalfdats smvfyeamkn evvipagall
      481 ppllyspqgv paynygsfgq yvavemargf glrgknsnye dqlnpwvnse lkeyyesvks
      541 clkeswdvvk ndhlpfhkpp redssfllde etafsdllgt rvayeayrkv dvklrmlsvd
      601 pdrlspparq ffvgscvkwc ldansnlvny agpfrcvlpl vhigefsdsi ncardskyas
      661 mikcslh
//
LOCUS       XP_077484706             180 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094653 [Amblyomma americanum].
ACCESSION   XP_077484706
VERSION     XP_077484706.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628580.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..180
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..180
                     /product="uncharacterized protein LOC144094653"
                     /calculated_mol_wt=18634
     CDS             1..180
                     /gene="LOC144094653"
                     /coded_by="XM_077628580.1:84..626"
                     /db_xref="GeneID:144094653"
ORIGIN      
        1 mnasclifvv ilvaaakgsd elcdmadnqk vqavqcitsh vsaqmkskwe alkqtfgsqd
       61 dliatnklce lrkgaaesga ktsiftqaee deikaafeac kmqaqpaqpa qpaqpaqsaq
      121 paqpaqpaqp aqpaqpaqpa ppaqpaqpaq paqpaqsaqp aqpakqvqqp aealpaqakp
//
LOCUS       XP_077484716             571 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094664 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077484716
VERSION     XP_077484716.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628590.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..571
                     /product="uncharacterized protein LOC144094664 isoform X1"
                     /calculated_mol_wt=63554
     CDS             1..571
                     /gene="LOC144094664"
                     /coded_by="XM_077628590.1:28..1743"
                     /db_xref="GeneID:144094664"
ORIGIN      
        1 megilsisve damspdhnep dpkykrnvff savlslllla sivatvhflp iyaesgeplv
       61 sqeatadhii afasskhrte ppvvytlppp vkskalvpig ghgledsngd lfaddadegv
      121 pshtlgksns tpplplplld ekdmllelee rvehsvhvts tgdprravgk kqaalehkph
      181 dqrapinrsl elrtpatvfk kstkpstksg ftarilvcfi sgntptsmda lppsgicqyv
      241 iyafagyakg rlrfvypktf ekflehargs lsvggegsca wgvtldaaea msgqtgvwge
      301 nrhqfalhvh ellaahgiss fgladvhtta adfidtandy ldfytnfhgl lhklrvpsgl
      361 kptlffgmrv lgmfysnrda frdrvknvlq fvdifiyqth vsritktcvs vfpsnryypg
      421 mddqdsfdda lrtltppivy kddfhlvlsl slsllrftmk gpdieeirrp cknmislpfk
      481 eycnttgfge daedelqigv yrfnerkavv dtfetadvie fkmndvmrey qlngldvgfa
      541 afylehedyr gecgnsfarl rmmskclrnf v
//
LOCUS       XP_077484721             570 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094664 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077484721
VERSION     XP_077484721.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628595.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..570
                     /product="uncharacterized protein LOC144094664 isoform X2"
                     /calculated_mol_wt=63483
     CDS             1..570
                     /gene="LOC144094664"
                     /coded_by="XM_077628595.1:28..1740"
                     /db_xref="GeneID:144094664"
ORIGIN      
        1 megilsisve damspdhnep dpkykrnvff savlslllla sivatvhflp iyesgeplvs
       61 qeatadhiia fasskhrtep pvvytlpppv kskalvpigg hgledsngdl faddadegvp
      121 shtlgksnst pplplpllde kdmlleleer vehsvhvtst gdprravgkk qaalehkphd
      181 qrapinrsle lrtpatvfkk stkpstksgf tarilvcfis gntptsmdal ppsgicqyvi
      241 yafagyakgr lrfvypktfe kflehargsl svggegscaw gvtldaaeam sgqtgvwgen
      301 rhqfalhvhe llaahgissf gladvhttaa dfidtandyl dfytnfhgll hklrvpsglk
      361 ptlffgmrvl gmfysnrdaf rdrvknvlqf vdifiyqthv sritktcvsv fpsnryypgm
      421 ddqdsfddal rtltppivyk ddfhlvlsls lsllrftmkg pdieeirrpc knmislpfke
      481 ycnttgfged aedelqigvy rfnerkavvd tfetadvief kmndvmreyq lngldvgfaa
      541 fylehedyrg ecgnsfarlr mmskclrnfv
//
LOCUS       XP_077484729             428 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094664 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077484729
VERSION     XP_077484729.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628603.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..428
                     /product="uncharacterized protein LOC144094664 isoform X3"
                     /calculated_mol_wt=48309
     CDS             1..428
                     /gene="LOC144094664"
                     /coded_by="XM_077628603.1:393..1679"
                     /db_xref="GeneID:144094664"
ORIGIN      
        1 mlleleerve hsvhvtstgd prravgkkqa alehkphdqr apinrslelr tpatvfkkst
       61 kpstksgfta rilvcfisgn tptsmdalpp sgicqyviya fagyakgrlr fvypktfekf
      121 lehargslsv ggegscawgv tldaaeamsg qtgvwgenrh qfalhvhell aahgissfgl
      181 advhttaadf idtandyldf ytnfhgllhk lrvpsglkpt lffgmrvlgm fysnrdafrd
      241 rvknvlqfvd ifiyqthvsr itktcvsvfp snryypgmdd qdsfddalrt ltppivykdd
      301 fhlvlslsls llrftmkgpd ieeirrpckn mislpfkeyc nttgfgedae delqigvyrf
      361 nerkavvdtf etadviefkm ndvmreyqln gldvgfaafy lehedyrgec gnsfarlrmm
      421 skclrnfv
//
LOCUS       XP_077484744             146 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094697 [Amblyomma americanum].
ACCESSION   XP_077484744
VERSION     XP_077484744.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628618.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..146
                     /product="uncharacterized protein LOC144094697"
                     /calculated_mol_wt=15912
     CDS             1..146
                     /gene="LOC144094697"
                     /coded_by="XM_077628618.1:254..694"
                     /db_xref="GeneID:144094697"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrppfa eftsvlccqr pasigpcvlp afgcvippdp
       61 fmlktkscme vvqatklrvi fardleqagk lvdgqwysls kasffsktkt iflgswamyl
      121 gtcgqdnagq hasasskkkh etvnky
//
LOCUS       XP_077484766             373 aa            linear   INV 25-NOV-2025
DEFINITION  pancreatic triacylglycerol lipase-like [Amblyomma americanum].
ACCESSION   XP_077484766
VERSION     XP_077484766.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628640.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..373
                     /product="pancreatic triacylglycerol lipase-like"
                     /calculated_mol_wt=41062
     Region          86..366
                     /region_name="Abhydrolase"
                     /note="alpha/beta hydrolases; cl21494"
                     /db_xref="CDD:473884"
     Site            200..204
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238382"
     Site            order(202,298)
                     /site_type="other"
                     /note="catalytic triad"
                     /db_xref="CDD:238382"
     CDS             1..373
                     /gene="LOC144094728"
                     /coded_by="XM_077628640.1:279..1400"
                     /db_xref="GeneID:144094728"
ORIGIN      
        1 myfkivmflc lilyscfkqg ataekgssad epemdfeaih qniehvmnqq mphkdkvcyd
       61 vvgcfdqkgl lahiqllpkp pseiatrfql hsrsgsdvld iyatggmtls rayrpgaplk
      121 vlahglggsg nltylhqmkd aflrledgnv vvvdwalgas lrdfvqacan aelvgrqvaf
      181 lvsslthngl vlpwnvhlig fslgaqlmgf aarrlartyg lkagrltald caaplfeprg
      241 lhprkqdvly ldavhtsagt nllmghvglr kpfghvdfyp nggiqqkgce gkgltcshqr
      301 aylffvesls qwtrcrfksi awaegcrffs gkrcgrgptt glefgrmgyf spstkargvh
      361 yletnpeppf cke
//
LOCUS       XP_077484778             340 aa            linear   INV 25-NOV-2025
DEFINITION  meiotic recombination protein DMC1/LIM15 homolog [Amblyomma
            americanum].
ACCESSION   XP_077484778
VERSION     XP_077484778.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628652.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..340
                     /product="meiotic recombination protein DMC1/LIM15
                     homolog"
                     /calculated_mol_wt=37394
     Region          23..338
                     /region_name="recomb_DMC1"
                     /note="meiotic recombinase Dmc1; TIGR02238"
                     /db_xref="CDD:131292"
     CDS             1..340
                     /gene="LOC144094739"
                     /coded_by="XM_077628652.1:256..1278"
                     /db_xref="GeneID:144094739"
ORIGIN      
        1 mgdqvveeai gqmiddeeff qdidilqnhg invtdikklk tagictvkgv qmttrkrlca
       61 ikgiseakvd kikeivgkis dcssfltale vcekrryvfr vttgskeldk lmgggvesma
      121 itevfgefrt gktqlshtlc vtcqlpgeng ysggkaifid tentfrpdrl rsiadrfdld
      181 hsavlenvly arvftsehqm emldqvaakf heeagvykll vvdsvmalfr vdfsgrgela
      241 erqqklaqml sklqkiseey nvavfitnqm tadpgasmsf qadpkkpigg hilahasttr
      301 ialrkgrgea riakiydspd qpeneatfai tpggiadate
//
LOCUS       XP_077484793             289 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094757 [Amblyomma americanum].
ACCESSION   XP_077484793
VERSION     XP_077484793.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628667.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..289
                     /product="uncharacterized protein LOC144094757"
                     /calculated_mol_wt=30104
     CDS             1..289
                     /gene="LOC144094757"
                     /coded_by="XM_077628667.1:105..974"
                     /db_xref="GeneID:144094757"
ORIGIN      
        1 mhvlakaall watlslslag tprlgsralv lfknlpnqkq tqqnhqqqvp gngfgppmvh
       61 pvyplnpmfp rgpvfppppp ylqpmhpflp ygphmqpiqp iqmfpihgpg llqapvplpg
      121 plhpmvtpiq ihslagnpgg nvaaglaatl pvnlanaiag ggtisagpty liqelrhhns
      181 pstttksqss snsgssnlnl qspekilflk dlksfsgpap aaknnkklvl ptpqdsfknl
      241 gpeilppivl nantlaqlgl gglsgpvtgg flpdggpdtl plkggnaiw
//
LOCUS       XP_077484801             249 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094766 [Amblyomma americanum].
ACCESSION   XP_077484801
VERSION     XP_077484801.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628675.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..249
                     /product="uncharacterized protein LOC144094766"
                     /calculated_mol_wt=27566
     CDS             1..249
                     /gene="LOC144094766"
                     /coded_by="XM_077628675.1:133..882"
                     /db_xref="GeneID:144094766"
ORIGIN      
        1 mgplwgvvvt wsllalcpgs esrstaahlw nrirqmafsg rqhhglqrpa tsmfvldanf
       61 ggpflyapqd slprmpfqkl yvynrpmgfr hwrrenrpsm qgrklskvkv vkkfvrqppi
      121 gnngvvllla pqtirppppl pspwrplpgp aaqgwalarg fppssrlqpp rkthvpkskt
      181 rprpvvyltg ahtsdpgahm aagtsmkdvt iihnsrkyti sasskarlkg hpglvvhvrg
      241 mgvhkrqlp
//
LOCUS       XP_077484808             177 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094776 [Amblyomma americanum].
ACCESSION   XP_077484808
VERSION     XP_077484808.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628682.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..177
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..177
                     /product="uncharacterized protein LOC144094776"
                     /calculated_mol_wt=16146
     CDS             1..177
                     /gene="LOC144094776"
                     /coded_by="XM_077628682.1:88..621"
                     /db_xref="GeneID:144094776"
ORIGIN      
        1 mgglssfgsf ghsgggfgga gfgtggfggg ggfgsgkrqq vyiveyvrsn qkghgsgfgk
       61 gsgggfgsgp vyiiqqiggg gggfggggfg agwkpggmgg fggggwkatg gggwkatgss
      121 pgmwstvggf gggawkpagg gaggpwkagg gngitdtyvv itsktsgggg gagagww
//
LOCUS       XP_077484812             307 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094787 [Amblyomma americanum].
ACCESSION   XP_077484812
VERSION     XP_077484812.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628686.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..307
                     /product="uncharacterized protein LOC144094787"
                     /calculated_mol_wt=25207
     CDS             1..307
                     /gene="LOC144094787"
                     /coded_by="XM_077628686.1:1579..2502"
                     /db_xref="GeneID:144094787"
ORIGIN      
        1 mrpmivlasl igmalagnlg gggggggwqa ggggggwqgg gggggwqagg ggggwqaggg
       61 gggggggkhv yiirkvsggg gsgggggggg wqpsgggwml gggggsggwq lgggggggwq
      121 pagggggggw qaggggggwq aggggggwqa gggggggwps ggggggwqag gggggwqagg
      181 gggggggggk hvfvvrttsk fggggghggg gggyggggfg ggghgggggh ggggggggkh
      241 vflvktttts sggggghggg ggggwqaggg gggwqagggg ggwqaggggg gwqagggggg
      301 gggggww
//
LOCUS       XP_077484820             169 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094799 [Amblyomma americanum].
ACCESSION   XP_077484820
VERSION     XP_077484820.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628694.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..169
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..169
                     /product="uncharacterized protein LOC144094799"
                     /calculated_mol_wt=14097
     CDS             1..169
                     /gene="LOC144094799"
                     /coded_by="XM_077628694.1:311..820"
                     /db_xref="GeneID:144094799"
ORIGIN      
        1 mrslifasav vgvvlagdig gggfggggfg gggfggggfg gggygggghg gggggggavh
       61 rfvittktts ggggggyggg gfggggfggg gfgggggggg ggfsgvgvgs askfkgstiy
      121 vlkprvtggg gggyggggyg gggfgggggw apsggggggg wqpsgggwq
//
LOCUS       XP_077484829             161 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094806 [Amblyomma americanum].
ACCESSION   XP_077484829
VERSION     XP_077484829.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628703.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..161
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..161
                     /product="uncharacterized protein LOC144094806"
                     /calculated_mol_wt=17785
     CDS             1..161
                     /gene="LOC144094806"
                     /coded_by="XM_077628703.1:425..910"
                     /db_xref="GeneID:144094806"
ORIGIN      
        1 mlrtvitlla agtalaayld dekpyerseg agmeresayh yerdsrhqrl pprysseraw
       61 ggrgpvagra arvrpgpiyi irtlnpepgp glleaplepw qappvvyraa pkaiaegsnv
      121 qyvvfvdrsa vpidgptlvd ayhgrlrkiq rwnpdlykak a
//
LOCUS       XP_077484836             276 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094831 [Amblyomma americanum].
ACCESSION   XP_077484836
VERSION     XP_077484836.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628710.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..276
                     /product="uncharacterized protein LOC144094831"
                     /calculated_mol_wt=24646
     CDS             1..276
                     /gene="LOC144094831"
                     /coded_by="XM_077628710.1:1..831"
                     /db_xref="GeneID:144094831"
ORIGIN      
        1 mrlwvatsva laaatialas pfgggggggg wkmmgggggf kggmgggggm ggmknkfrtg
       61 svylikelhp iggqgkggfm mmggggggwk pmmggggggw kpmgggggwk mggggnggwm
      121 gggggggapg wmrmatvvln hgggsggggw kmgggsgggg wkmgggsggw kmgggggsgg
      181 ggwkmgggsg gwkmgggggg gwkmgggggg sggkagttyi iikkgssssg gksggggvgg
      241 hlssltslfs ghgggggggg hgggssgggg gggwwm
//
LOCUS       XP_077484850             138 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094835 [Amblyomma americanum].
ACCESSION   XP_077484850
VERSION     XP_077484850.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628724.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..138
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..138
                     /product="uncharacterized protein LOC144094835"
                     /calculated_mol_wt=14669
     CDS             1..138
                     /gene="LOC144094835"
                     /coded_by="XM_077628724.1:162..578"
                     /db_xref="GeneID:144094835"
ORIGIN      
        1 mtpakrtqta fllctallll stgratvaat fpgayfypra ylsgpaqpta spstqhqfya
       61 asspvattgs skggttyvir slregemikp ettsmaggfd ayasspvlsa afpnyerhfn
      121 fvrpylsgpv vapwhfvh
//
LOCUS       XP_077484858             126 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094846 [Amblyomma americanum].
ACCESSION   XP_077484858
VERSION     XP_077484858.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628732.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..126
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..126
                     /product="uncharacterized protein LOC144094846"
                     /calculated_mol_wt=13546
     CDS             1..126
                     /gene="LOC144094846"
                     /coded_by="XM_077628732.1:471..851"
                     /db_xref="GeneID:144094846"
ORIGIN      
        1 mtshikisal allacclalv pshasieqga gqhmyaggee yphgfmvsti hesddrhghr
       61 srsyghghrd yvnepeyvvr npygallqqs esqhlyaagg alhggglghd haaffpghmd
      121 gylmar
//
LOCUS       XP_077484866             497 aa            linear   INV 25-NOV-2025
DEFINITION  endochitinase-like [Amblyomma americanum].
ACCESSION   XP_077484866
VERSION     XP_077484866.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628740.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..497
                     /product="endochitinase-like"
                     /calculated_mol_wt=55289
     Region          153..>306
                     /region_name="GH18_chitinase-like"
                     /note="The GH18 (glycosyl hydrolase, family 18) type II
                     chitinases hydrolyze chitin, an abundant polymer of
                     beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a
                     major component of the cell wall of fungi and the
                     exoskeleton of arthropods. Chitinases have...; cl10447"
                     /db_xref="CDD:471972"
     Region          <409..490
                     /region_name="GH18_chitinase-like"
                     /note="The GH18 (glycosyl hydrolase, family 18) type II
                     chitinases hydrolyze chitin, an abundant polymer of
                     beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a
                     major component of the cell wall of fungi and the
                     exoskeleton of arthropods. Chitinases have...; cl10447"
                     /db_xref="CDD:471972"
     CDS             1..497
                     /gene="LOC144094857"
                     /coded_by="XM_077628740.1:120..1613"
                     /db_xref="GeneID:144094857"
ORIGIN      
        1 mrtdeyvspc weesygqsgt wagpdldapp rgphenlmrw mflvlsallt tasvallata
       61 llqpsgsghh hrthttphag pppavqgpkg hkegaavqaa knaaasnrvv vvhapagnfm
      121 gkqgrarnep garmaapirr kherkevkpe ihcvynnkap eyplpftpsd ipvglcasai
      181 ycclgptsdg tnltflhvdp kngpldletf ssvlhsmdpt mrrhiavggp rlnyahlara
      241 lrdsasrsef vnsvmgwlnr phlesfgfvl hiiqpekvsk hrsldalvle lssklnsrtf
      301 mltlplngev esryfhplty ksvnafikms hrfgtpiigs cpnpvrglrt hslqkvirdv
      361 kdkyqdrwds direktlftv slagyiywvr ngsltgltkg rvekhalsgy wdicrkvdda
      421 swnhkyiret gcltawsgrn yvsslspksf tfldgtremk gvvvfdiekd dfkgmcgdpy
      481 pmlralresl tkfeeik
//
LOCUS       XP_077484874             240 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094867 [Amblyomma americanum].
ACCESSION   XP_077484874
VERSION     XP_077484874.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628748.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..240
                     /product="uncharacterized protein LOC144094867"
                     /calculated_mol_wt=19091
     CDS             1..240
                     /gene="LOC144094867"
                     /coded_by="XM_077628748.1:57..779"
                     /db_xref="GeneID:144094867"
ORIGIN      
        1 mrslillsav glalagnlgg ggggfggggf gggghggggg fgggggghhg ggggagggav
       61 vstsvveiks lgstgggggg gggghhgggg fggggfgggg fggggfgggg hhggggggvg
      121 ggqvvgtyka gpaylvkalq piggggvggv gggvhgggfg ggglggggfg ggghgggfgg
      181 gggvgggtat yhvtvkgggg gavggggygg ggfgggghgg gggghgghgg ggggggggww
//
LOCUS       XP_077484908             407 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094916 [Amblyomma americanum].
ACCESSION   XP_077484908
VERSION     XP_077484908.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628782.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..407
                     /product="uncharacterized protein LOC144094916"
                     /calculated_mol_wt=44288
     CDS             1..407
                     /gene="LOC144094916"
                     /coded_by="XM_077628782.1:127..1350"
                     /db_xref="GeneID:144094916"
ORIGIN      
        1 mrgarrlvrc elvaiavval llasravadg aevkantdka eakepaggdl igagsgfkhr
       61 rinkassvev dtpldtegfq hqgayerpgy vkgyaklrga tygkisysrd dslrpergyd
      121 spervnygkv kyshggddaa gyrdsddsra gaavydsnay sndgyltpiy nhgdyvrasr
      181 hrgsyrhksr prgmsytpvd ygrevypyeq gdqqtggqvs yssrphgnki sgstsvfdgg
      241 synypdadry ppvggdydfg tqkpllqpgs siqgslsifg gygqgngnlr yrdfvpyygs
      301 dgyysrhdsa ayglqnygst vreaqpkpye dysnnlsdsg ldgayagpsq rygakfpsgk
      361 kthkhhgnnr sglgayivgv eyigrtpllv qtqpsrsada kaeqywq
//
LOCUS       XP_077484938             523 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094940 [Amblyomma americanum].
ACCESSION   XP_077484938
VERSION     XP_077484938.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628812.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..523
                     /product="uncharacterized protein LOC144094940"
                     /calculated_mol_wt=55922
     Region          56..>107
                     /region_name="AdoMet_MTases"
                     /note="S-adenosylmethionine-dependent methyltransferases
                     (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes
                     that use S-adenosyl-L-methionine (SAM or AdoMet) as a
                     substrate for methyltransfer, creating the product
                     S-adenosyl-L-homocysteine (AdoHcy); cl17173"
                     /db_xref="CDD:473071"
     Region          249..273
                     /region_name="zf-U11-48K"
                     /note="U11-48K-like CHHC zinc finger; pfam05253"
                     /db_xref="CDD:461603"
     CDS             1..523
                     /gene="LOC144094940"
                     /coded_by="XM_077628812.1:18..1589"
                     /db_xref="GeneID:144094940"
ORIGIN      
        1 mvplvsgtaa pgseprrpas scteltvtfe erderaeree rgeqddferp rgralgrraw
       61 rggsrgaggp rgaeesrgag eprgsrgprv sggprvsggp rapggsgise gprvsgesrg
      121 sggprgsrgp rgvggprgae vprssgesrg sgrsrgggrp mgtggpsgsa ersggargra
      181 rgggrgrggl arassqprss qprssqprss rtrqlpqrta qplafdpepe eknmslvtde
      241 wslwgakdmv rcpfnpshqv lrsqranhir fcksringrh grqrrgeepq llppeenwep
      301 divpgqpgya pltspeqpif revnsmshse kkqyyvellk qykdahggts keenkeqnqa
      361 egvsslketa dcasspkqaa vsasacgvpp saatamdarr sekgepdrtn sttrllqqqq
      421 qqqqpvqatl valvhpqplm gdseaeqmac spddivaine lrtlletmqv athydpytde
      481 asyqaagrgs aiprtsvygw ivssddqggv fyqsprgrgr ply
//
LOCUS       XP_077484957              93 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094979 [Amblyomma americanum].
ACCESSION   XP_077484957
VERSION     XP_077484957.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628831.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..93
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..93
                     /product="uncharacterized protein LOC144094979"
                     /calculated_mol_wt=8925
     CDS             1..93
                     /gene="LOC144094979"
                     /coded_by="XM_077628831.1:30..311"
                     /db_xref="GeneID:144094979"
ORIGIN      
        1 mrlllasllv ctlvgacfag lvsygggggg ggygkslpgp sfliktlhhv skvshggpil
       61 gnydlgvsyg hgggyggfgg gygggyggyg wkg
//
LOCUS       XP_077484961             130 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094988 [Amblyomma americanum].
ACCESSION   XP_077484961
VERSION     XP_077484961.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628835.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..130
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..130
                     /product="uncharacterized protein LOC144094988"
                     /calculated_mol_wt=12177
     CDS             1..130
                     /gene="LOC144094988"
                     /coded_by="XM_077628835.1:228..620"
                     /db_xref="GeneID:144094988"
ORIGIN      
        1 mrtmifalfv salvgvslag lvgygggygg gygggfgggy ggglaygggl ghgvgygksl
       61 pgpsfliksl hhvskvshgg pilanydlgv aygggygggy gggygfgggy ggfggyggyg
      121 hgglgyglkg
//
LOCUS       XP_077484967              87 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144094999 [Amblyomma americanum].
ACCESSION   XP_077484967
VERSION     XP_077484967.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628841.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..87
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..87
                     /product="uncharacterized protein LOC144094999"
                     /calculated_mol_wt=8162
     CDS             1..87
                     /gene="LOC144094999"
                     /coded_by="XM_077628841.1:262..525"
                     /db_xref="GeneID:144094999"
ORIGIN      
        1 mkatlllvcl aavfalaaag gfgyggyggy gggfgrfggg yggyglgrgl gygggyggyg
       61 ggyggygggf rgyggyggyg ygkgfyg
//
LOCUS       XP_077484976             249 aa            linear   INV 25-NOV-2025
DEFINITION  procollagen C-endopeptidase enhancer 1-like [Amblyomma americanum].
ACCESSION   XP_077484976
VERSION     XP_077484976.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628850.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..249
                     /product="procollagen C-endopeptidase enhancer 1-like"
                     /calculated_mol_wt=26650
     Region          22..128
                     /region_name="CUB"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; cd00041"
                     /db_xref="CDD:238001"
     Site            order(29,31,33,62,67,104,126,128)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     CDS             1..249
                     /gene="LOC144095030"
                     /coded_by="XM_077628850.1:40..789"
                     /db_xref="GeneID:144095030"
ORIGIN      
        1 malplllsla vglaslapaq pcgktltrsg rltspgypma yppnvrckyt lrpqgagvca
       61 velkfeeldv dghaprcdag davllrstge rfcgvepppp lvlpisaegl qmefyangqt
      121 sgtgfalrlt qienscpkkg spcggtfake hfrlegpsap ggaclyllrk fdptvcqvqv
      181 rfkrfsmgpd gacdkprllv agrslcgtra pedvetfdfm sqtlaflyqp gdnpqgdkfv
      241 levfqekcd
//
LOCUS       XP_077484982             595 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095043 [Amblyomma americanum].
ACCESSION   XP_077484982
VERSION     XP_077484982.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628856.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..595
                     /product="uncharacterized protein LOC144095043"
                     /calculated_mol_wt=65485
     Region          407..591
                     /region_name="aden_kin_iso1"
                     /note="adenylate kinase, isozyme 1 subfamily; TIGR01360"
                     /db_xref="CDD:130427"
     CDS             1..595
                     /gene="LOC144095043"
                     /coded_by="XM_077628856.1:170..1957"
                     /db_xref="GeneID:144095043"
ORIGIN      
        1 maaehpqrtk lryqsnstfv lkpksnvrvr pprageqkaa skmvmsasad gsdtattavt
       61 avpvsstint psartsymal emmseapatm vgvssmgpmg yptsggspit tfgsmhpglf
      121 sdlrgeetfe aefgsdgrrp rwrssamrsr srraaggsss rmvhpaerdf daddeserrf
      181 edrrarrrrq grrgrrssid deeadmddga ggrlvctcga sssggggggg vgvggggggi
      241 fmgqspedfe dlyvlkktge rkkkkkkkkk kppppppppp pppppglmgm lcgllgskpp
      301 pppppppppp eesssesese yeseyelrrl drqqlehfcr qidfrqfikl pepekkeekk
      361 eekkedkkee kkedkkadkk eekkedrpas dvkkpdappk egpldvkvpv ifvfggpgsg
      421 kgtqcdkigq kygfthissg dllraevqsg sdrgkemnei mtkgelvpld ivllllkeai
      481 kkdlekakgf lidgyprnie qgdrfeaevc kctnvlffev kdetmkerll krgetsgrad
      541 dneetitkri klfheesepv ldkykglvhk vsaeedpdkv feavvpffdd ltkpk
//
LOCUS       XP_077484987             183 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger protein 711-like [Amblyomma americanum].
ACCESSION   XP_077484987
VERSION     XP_077484987.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628861.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="zinc finger protein 711-like"
                     /calculated_mol_wt=20255
     Region          60..81
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(60,63,76,81)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          93..113
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(93,96,109,113)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(98,100,102,104..105,108..109,112,124,126,130..131,
                     134..135,138,152,154,156,158..159,162..163)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          119..139
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          147..163
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..183
                     /gene="LOC144095051"
                     /coded_by="XM_077628861.1:77..628"
                     /db_xref="GeneID:144095051"
ORIGIN      
        1 mdpqaaastt agsppsldgt eakrrvsdeg lnaapeelrh asdqplrled ireggtvhkc
       61 recmkvfqlp srlmahgifk hrkkasllkh pacpvcdkkc ksraimalhl gthigerqck
      121 kcgaqfasar savvhrrfhl tggnfqcdic gklfprkcqr nehcfwehgq vhqpatsdys
      181 fse
//
LOCUS       XP_077484995             185 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095060 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077484995
VERSION     XP_077484995.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628869.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..185
                     /product="uncharacterized protein LOC144095060 isoform X1"
                     /calculated_mol_wt=20483
     Region          95..115
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(100,102,104,106..107,110..111,114,126,128,132..133,
                     136..137,140,154,156,158,160..161,164..165,168)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          121..141
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          <148..>175
                     /region_name="KREPA"
                     /note="Kinetoplastid RNA Editing Protein A (KREPA);
                     cl49620"
                     /db_xref="CDD:483960"
     Region          149..168
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..185
                     /gene="LOC144095060"
                     /coded_by="XM_077628869.1:35..592"
                     /db_xref="GeneID:144095060"
ORIGIN      
        1 mrahrqlsnd crsatphllr ralarlvear eaaclqvrgi tqqlrrlqvn rpadesamyk
       61 crdcqlqfpl asklmahnvy rhsdviieqr aklpcpickv rcrtkrtmsa hlgthlgerl
      121 cekcgaefas agaaaahelf hptagnfacs qcqrqfarqs glvehsrveh glvlphgssp
      181 clpge
//
LOCUS       XP_077484997              78 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095060 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077484997
VERSION     XP_077484997.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628871.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..78
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..78
                     /product="uncharacterized protein LOC144095060 isoform X2"
                     /calculated_mol_wt=8352
     CDS             1..78
                     /gene="LOC144095060"
                     /coded_by="XM_077628871.1:411..647"
                     /db_xref="GeneID:144095060"
ORIGIN      
        1 mkqsairyhn ihhgnvcasr rsghqgyera ciehvvtase slvhnadpsr pitlavglrf
       61 qqsnggcgat slrqrcvg
//
LOCUS       XP_077485010             188 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095083 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077485010
VERSION     XP_077485010.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628884.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..188
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..188
                     /product="uncharacterized protein LOC144095083 isoform X1"
                     /calculated_mol_wt=21104
     Region          98..118
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(98,101,114,118)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(103,105,107,109..110,113..114,117,129,131,135..136,
                     139..140,143,157,159,161,163..164,167..168)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          124..144
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          152..169
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..188
                     /gene="LOC144095083"
                     /coded_by="XM_077628884.1:23..589"
                     /db_xref="GeneID:144095083"
ORIGIN      
        1 mdqkssaslp ivgqpsraea gakqreidvn vdwvlppeep rhicekpmcl heidvpgqgt
       61 lylcrhcrvt fklesrllah avfrhggryi gcartlacrm cpqrfkrqrt malhigthlg
      121 erqcskcgae fasaaaatah ktfhrtggnf qcgfcgvlfi qksdrdehlg lehsrqrrmk
      181 rprfpttv
//
LOCUS       XP_077485017             155 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095083 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077485017
VERSION     XP_077485017.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628891.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..155
                     /product="uncharacterized protein LOC144095083 isoform X2"
                     /calculated_mol_wt=17604
     Region          65..85
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(65,68,81,85)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(70,72,74,76..77,80..81,84,96,98,102..103,106..107,
                     110,124,126,128,130..131,134..135)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          91..111
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          119..136
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..155
                     /gene="LOC144095083"
                     /coded_by="XM_077628891.1:140..607"
                     /db_xref="GeneID:144095083"
ORIGIN      
        1 mpesreprhi cekpmclhei dvpgqgtlyl crhcrvtfkl esrllahavf rhggryigca
       61 rtlacrmcpq rfkrqrtmal higthlgerq cskcgaefas aaaatahktf hrtggnfqcg
      121 fcgvlfiqks drdehlgleh srqrrmkrpr fpttv
//
LOCUS       XP_077485028             230 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095100 [Amblyomma americanum].
ACCESSION   XP_077485028
VERSION     XP_077485028.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628902.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..230
                     /product="uncharacterized protein LOC144095100"
                     /calculated_mol_wt=25241
     CDS             1..230
                     /gene="LOC144095100"
                     /coded_by="XM_077628902.1:11..703"
                     /db_xref="GeneID:144095100"
ORIGIN      
        1 mffssrrilr slrpletqal pvlesagfag kgqgrrqped veqssdegpf vdddgrfqlv
       61 alvsalmart dfashaesik ssalfmdhwp aayanmnste wkwlamlleg ggsfshrtlr
      121 vlpvpavvcg rvvpctawdy nlltrqailq gsallmdhwp aayasmkdae wkraillleg
      181 dssnsrcmpl kptvpavpyg ravtstdpdh npalrgsnai sewslvcdrh
//
LOCUS       XP_077485039             395 aa            linear   INV 25-NOV-2025
DEFINITION  lysosomal aspartic protease-like [Amblyomma americanum].
ACCESSION   XP_077485039
VERSION     XP_077485039.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628913.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..395
                     /product="lysosomal aspartic protease-like"
                     /calculated_mol_wt=42888
     Region          63..393
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(87,89,91,129..131,177)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133137"
     Site            87..89
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133137"
     Site            87
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133137"
     Site            order(129..132,136..139)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133137"
     CDS             1..395
                     /gene="LOC144095111"
                     /coded_by="XM_077628913.1:93..1280"
                     /db_xref="GeneID:144095111"
ORIGIN      
        1 mtqpqlaalt lllalpvstl aqvrvplrrm rsvretfeaf gipsatlesg vgrsevcvqp
       61 lkdymdaqyy gdisigtppq yfrvlfdtgs sdlwvpsvnc terdricrlh qlynpaksct
      121 hvrngtrvel vygsgpvhge vtvdvitigk htvkgqsfvq sdnrrgtafa yakfdgilgl
      181 aypeqsvlga tpvfdnmiqq gvvqnpvfsv flkrggagpa gqptgiegge vyfggidaah
      241 ysgeilyvpv skkgywqltv gsvklgtdqf cptgceaivd tgssvitgpa eevnrinrll
      301 gaapfslels qvdcsrvskl pdltftvggk pltvrpqdyi vklrqsyktc cvvfikpndf
      361 dsghgpvwnl gdpfigryft vfdrgndrlg faear
//
LOCUS       XP_077485050             252 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095123 [Amblyomma americanum].
ACCESSION   XP_077485050
VERSION     XP_077485050.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628924.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..252
                     /product="uncharacterized protein LOC144095123"
                     /calculated_mol_wt=27728
     Region          11..92
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..252
                     /gene="LOC144095123"
                     /coded_by="XM_077628924.1:218..976"
                     /db_xref="GeneID:144095123"
ORIGIN      
        1 maavaavsqp rnlwseretw vlidlweerl ddlsrqkrns vvyceiaeal raagfgrsta
       61 evrhkirnlt rtyrnytrtg qatgsgagdw shlqrihsff gsslplsvas laqkpgcsdg
      121 tavehctdkm grcpnstdss hataqsscdg veaseetgsr prssegalag sasetaedgp
      181 lphlpspqlq rttrkrkrta pmsefeqall tehrllrksf evayarlldi terqlklqer
      241 ladaitesfk sp
//
LOCUS       XP_077485061             126 aa            linear   INV 25-NOV-2025
DEFINITION  putative peptidyl-tRNA hydrolase PTRHD1 [Amblyomma americanum].
ACCESSION   XP_077485061
VERSION     XP_077485061.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628935.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..126
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..126
                     /product="putative peptidyl-tRNA hydrolase PTRHD1"
                     /calculated_mol_wt=13653
     Region          12..125
                     /region_name="PTH2_like"
                     /note="Peptidyl-tRNA hydrolase, type 2 (PTH2)_like .
                     Peptidyl-tRNA hydrolase activity releases tRNA from the
                     premature translation termination product peptidyl-tRNA.
                     Two structurally different enzymes have been reported to
                     encode such activity, Pth present in...; cd02429"
                     /db_xref="CDD:239107"
     Site            order(30,37..39,42,45..46,49,60,66,125)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239107"
     Site            order(32,36,39,66,103)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:239107"
     Site            order(32,36,39,66,103)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:239107"
     CDS             1..126
                     /gene="LOC144095134"
                     /coded_by="XM_077628935.1:42..422"
                     /db_xref="GeneID:144095134"
ORIGIN      
        1 maasatsagk vivqyvvvrs dlltelnwpv gaviaqacha ctaalqlfgd dvntiayten
       61 ldsmhkvvla aptepklldl cqalrdanid yklwieqpen iptcvatkpy pkadvqaffk
      121 gfklfk
//
LOCUS       XP_077485072             360 aa            linear   INV 25-NOV-2025
DEFINITION  adenosine receptor A3-like [Amblyomma americanum].
ACCESSION   XP_077485072
VERSION     XP_077485072.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628946.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..360
                     /product="adenosine receptor A3-like"
                     /calculated_mol_wt=40232
     Region          32..311
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          32..53
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410628"
     Region          62..87
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410628"
     Region          103..125
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410628"
     Region          148..164
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410628"
     Region          186..208
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410628"
     Region          238..263
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410628"
     Region          276..304
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410628"
     CDS             1..360
                     /gene="LOC144095145"
                     /coded_by="XM_077628946.1:1179..2261"
                     /db_xref="GeneID:144095145"
ORIGIN      
        1 msainitsed lnvtarspld lardvyrlav pvmlvaclms mvfnlvives vhwlrrspsp
       61 tlcfslslsa anayaslvlg iglvvnsllp tlyqiqppac walsleafrl tgliasvfhl
      121 lalavnhyig ilrplhyaat vtrrtvwltt aamwllplsf flvyftsvpg qgfqvascry
      181 dfilwstfrv tlsglffvpl ilmcfiythi fllvrrhqmg vgisaqipgq lrksvkavyt
      241 tllilgtyli gwmpavlfyv ltcldcplpi tslsldvrlt fgiltntlvl iksfvdpvvy
      301 vvrmreiqea fmlmwksrcg lvdvarssvd lmrmahsrtd tqrittngsq hkqrsivqfs
//
LOCUS       XP_077485080             332 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier family 25 member 35-like [Amblyomma americanum].
ACCESSION   XP_077485080
VERSION     XP_077485080.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628954.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..332
                     /product="solute carrier family 25 member 35-like"
                     /calculated_mol_wt=36683
     Region          18..286
                     /region_name="PTZ00169"
                     /note="ADP/ATP transporter on adenylate translocase;
                     Provisional"
                     /db_xref="CDD:240302"
     Region          115..214
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     CDS             1..332
                     /gene="LOC144095156"
                     /coded_by="XM_077628954.1:19..1017"
                     /db_xref="GeneID:144095156"
ORIGIN      
        1 mvvpgttttt tyvdgfrdvf egmvgptvat limhpfditr trmqvqgelk rrgyyvvtyk
       61 gtiqslrmms qidgpssvyr glapsiayrq vthglrlgff qviedcgltv denlepsvql
      121 saisgalcgg igaflgsplq lvkcqlqtra saqiavgyqh hyrssfeavr iiyrahglrr
      181 glwraaqanv lrlsigsgiq mgayvqakrk lsqytgddmh hlvtsvaaav yssiaaapav
      241 atldtiktrm ylqpvdrrgr giyyagvldc vrkiytmegv agysrgilia ffysitnsti
      301 tllswnelkw lrlasrerre lvieiiyqtg dd
//
LOCUS       XP_077485086             274 aa            linear   INV 25-NOV-2025
DEFINITION  aurora kinase A-B-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077485086
VERSION     XP_077485086.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628960.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..274
                     /product="aurora kinase A-B-like isoform X1"
                     /calculated_mol_wt=31718
     Region          10..262
                     /region_name="STKc_Aurora"
                     /note="Catalytic domain of the Serine/Threonine kinase,
                     Aurora kinase; cd14007"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,88..91,95,97,134,136,138..139,
                     141,152,155,169..172)
                     /site_type="active"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,89,91,95,138..139,141,152)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270909"
     Site            order(21,95,97,134,136,138,155,169..172)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270909"
     Site            order(151..160,163..172)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270909"
     CDS             1..274
                     /gene="LOC144095168"
                     /coded_by="XM_077628960.1:199..1023"
                     /db_xref="GeneID:144095168"
ORIGIN      
        1 msvkewclkd feigrplgkg kfgnvylare ketkfivalk vmfksqlqkn nvehqlqrei
       61 eiqshlrhpn ilclynwfhd etrvyliley apqgelyrhl tkakrftnqk aatyiyqlcn
      121 alkvchsqkv ihrdikpenl llgcngeiki adfgwsvhap ssrretfcgt ldylppemie
      181 gavynekvdh walgiliyef lvgkppfksr ttqqtyrric gakvkfprhv sadardiigk
      241 llkrlpadri sldevlthpw vtkhadttve cktl
//
LOCUS       XP_077485090             249 aa            linear   INV 25-NOV-2025
DEFINITION  aurora kinase A-B-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077485090
VERSION     XP_077485090.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628964.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..249
                     /product="aurora kinase A-B-like isoform X2"
                     /calculated_mol_wt=28903
     Region          10..240
                     /region_name="STKc_Aurora"
                     /note="Catalytic domain of the Serine/Threonine kinase,
                     Aurora kinase; cd14007"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,88..91,95,97,134,136,138..139,
                     141,152,155,169..172)
                     /site_type="active"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,89,91,95,138..139,141,152)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270909"
     Site            order(21,95,97,134,136,138,155,169..172)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270909"
     Site            order(151..160,163..172)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270909"
     CDS             1..249
                     /gene="LOC144095168"
                     /coded_by="XM_077628964.1:199..948"
                     /db_xref="GeneID:144095168"
ORIGIN      
        1 msvkewclkd feigrplgkg kfgnvylare ketkfivalk vmfksqlqkn nvehqlqrei
       61 eiqshlrhpn ilclynwfhd etrvyliley apqgelyrhl tkakrftnqk aatyiyqlcn
      121 alkvchsqkv ihrdikpenl llgcngeiki adfgwsvhap ssrretfcgt ldylppemie
      181 gavynekvdh walgiliyef lvgkppfksr ttqqtyrric gakvkfprhv sadardiigk
      241 rvpavqrem
//
LOCUS       XP_077485105             249 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095185 [Amblyomma americanum].
ACCESSION   XP_077485105
VERSION     XP_077485105.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628979.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..249
                     /product="uncharacterized protein LOC144095185"
                     /calculated_mol_wt=26444
     CDS             1..249
                     /gene="LOC144095185"
                     /coded_by="XM_077628979.1:1..750"
                     /db_xref="GeneID:144095185"
ORIGIN      
        1 mpkikieeka yarakasret kvlfenfgrc srqrtvpvpa asvassaast vssaassdga
       61 ttisdaffdi ralrneqvta vsdiaatpel pevapavssg fpcssasscd tasedpadcp
      121 npnpatesrd lvtalrawal kenishsals sllkllkahp fdttclpsda rtllrtpret
      181 mnisaltamp pgeychygle hqlrnmlshr slegsmlvea raadcskcat sysqqdccvd
      241 vtspfratv
//
LOCUS       XP_077485111             249 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095185 [Amblyomma americanum].
ACCESSION   XP_077485111
VERSION     XP_077485111.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628985.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..249
                     /product="uncharacterized protein LOC144095185"
                     /calculated_mol_wt=26444
     CDS             1..249
                     /gene="LOC144095185"
                     /coded_by="XM_077628985.1:1..750"
                     /db_xref="GeneID:144095185"
ORIGIN      
        1 mpkikieeka yarakasret kvlfenfgrc srqrtvpvpa asvassaast vssaassdga
       61 ttisdaffdi ralrneqvta vsdiaatpel pevapavssg fpcssasscd tasedpadcp
      121 npnpatesrd lvtalrawal kenishsals sllkllkahp fdttclpsda rtllrtpret
      181 mnisaltamp pgeychygle hqlrnmlshr slegsmlvea raadcskcat sysqqdccvd
      241 vtspfratv
//
LOCUS       XP_077485121             275 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095205 [Amblyomma americanum].
ACCESSION   XP_077485121
VERSION     XP_077485121.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077628995.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..275
                     /product="uncharacterized protein LOC144095205"
                     /calculated_mol_wt=30542
     CDS             1..275
                     /gene="LOC144095205"
                     /coded_by="XM_077628995.1:352..1179"
                     /db_xref="GeneID:144095205"
ORIGIN      
        1 mqehsyvall fyslcicgvi fififsarsq kenvaaldsc lnevtatayv tphaeepans
       61 aerlqgpgln gdplagqsts raatadlalq revppspsqq cgahncsrcl gyadfqrrvm
      121 snfaiirhtq teilellsgi trpesqvpen tdcvvsepfg tveelkafds gltperrnal
      181 iaqlqsygtk svpvtvrtmm aqlmkdslaa qfsmhgrkgk erfdalqlrs vifdsvrrtr
      241 hlqevthddv dtaikewlrr akercnqkls qhflq
//
LOCUS       XP_077485141             274 aa            linear   INV 25-NOV-2025
DEFINITION  aurora kinase A-B-like [Amblyomma americanum].
ACCESSION   XP_077485141
VERSION     XP_077485141.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629015.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..274
                     /product="aurora kinase A-B-like"
                     /calculated_mol_wt=31718
     Region          10..262
                     /region_name="STKc_Aurora"
                     /note="Catalytic domain of the Serine/Threonine kinase,
                     Aurora kinase; cd14007"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,88..91,95,97,134,136,138..139,
                     141,152,155,169..172)
                     /site_type="active"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,89,91,95,138..139,141,152)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270909"
     Site            order(21,95,97,134,136,138,155,169..172)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270909"
     Site            order(151..160,163..172)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270909"
     CDS             1..274
                     /gene="LOC144095226"
                     /coded_by="XM_077629015.1:199..1023"
                     /db_xref="GeneID:144095226"
ORIGIN      
        1 msvkewclkd feigrplgkg kfgnvylare ketkfivalk vmfksqlqkn nvehqlqrei
       61 eiqshlrhpn ilclynwfhd etrvyliley apqgelyrhl tkakrftnqk aatyiyqlcn
      121 alkvchsqkv ihrdikpenl llgcngeiki adfgwsvhap ssrretfcgt ldylppemie
      181 gavynekvdh walgiliyef lvgkppfksr ttqqtyrric gakvkfprhv sadardiigk
      241 llkrlpadri sldevlthpw vtkhadttve cktl
//
LOCUS       XP_077485155             148 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095244 [Amblyomma americanum].
ACCESSION   XP_077485155
VERSION     XP_077485155.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629029.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="uncharacterized protein LOC144095244"
                     /calculated_mol_wt=16759
     CDS             1..148
                     /gene="LOC144095244"
                     /coded_by="XM_077629029.1:380..826"
                     /db_xref="GeneID:144095244"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedrrr rhlnrrltat mkrlsgvhvl
       61 nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar pgevyvvhrc
      121 rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077485173             426 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095265 [Amblyomma americanum].
ACCESSION   XP_077485173
VERSION     XP_077485173.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629047.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..426
                     /product="uncharacterized protein LOC144095265"
                     /calculated_mol_wt=47969
     Region          180..364
                     /region_name="Galactosyl_T"
                     /note="Galactosyltransferase; cl21608"
                     /db_xref="CDD:473923"
     CDS             1..426
                     /gene="LOC144095265"
                     /coded_by="XM_077629047.1:68..1348"
                     /db_xref="GeneID:144095265"
ORIGIN      
        1 mnavpdcrrc atakmsacke tyhqcgrpfs rkhrkclrhf pliftslill taggcilrsp
       61 pvapsaaqga dvgrytglnv rplvgvaeqe aepgskdhgp papprppaal lqspkavpel
      121 paaawrddlq wlsrkipfpl dppntlepga dghrvrqlcs tgqadhlifv htdpthaqer
      181 rlfrttvghs riaslfkwtm lfliglrpda nvtdeaatfg dvvqlpfidh yrnlslkfly
      241 gmrwvmincp svrslvkidd dvfihpevld sylrkhinld errlhcsvfr gnavirnphs
      301 rhylsrsdyp kkyfpvhchg wfvvipasvm wdlyaasfrv pfhaiddayv tgdlakaasl
      361 shvdisavls aeedrllell rgeymcalft grtklsmrvt lwktlsyikk rqdldeamqh
      421 tlkafk
//
LOCUS       XP_077485183             221 aa            linear   INV 25-NOV-2025
DEFINITION  casein kinase II subunit beta-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077485183
VERSION     XP_077485183.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629057.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..221
                     /product="casein kinase II subunit beta-like isoform X1"
                     /calculated_mol_wt=25200
     Region          8..191
                     /region_name="CK_II_beta"
                     /note="Casein kinase II regulatory subunit; smart01085"
                     /db_xref="CDD:198153"
     CDS             1..221
                     /gene="LOC144095276"
                     /coded_by="XM_077629057.1:60..725"
                     /db_xref="GeneID:144095276"
ORIGIN      
        1 mssseevswi swfcglrgne ffcevdedyi qdkfnltgls eqvphyrqal dmildlvpdn
       61 elednanpsi lieqaaemly gliharyilt nqgialmiek yqhgdfgycp rvycesqqml
      121 piglsdvpge vmvklycpkc mdvygpkssl hhhtdgayfg tgfphmlfmv hpeyrpkspt
      181 nkfvprlygf kihplayqqe qqeaasfkap vramnygngk r
//
LOCUS       XP_077485190             214 aa            linear   INV 25-NOV-2025
DEFINITION  casein kinase II subunit beta-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077485190
VERSION     XP_077485190.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629064.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..214
                     /product="casein kinase II subunit beta-like isoform X2"
                     /calculated_mol_wt=24217
     Region          16..184
                     /region_name="CK_II_beta"
                     /note="Casein kinase II regulatory subunit; smart01085"
                     /db_xref="CDD:198153"
     CDS             1..214
                     /gene="LOC144095276"
                     /coded_by="XM_077629064.1:23..667"
                     /db_xref="GeneID:144095276"
ORIGIN      
        1 mplrgmvgrs vcrvsaevde dyiqdkfnlt glseqvphyr qaldmildlv pdnelednan
       61 psilieqaae mlyglihary iltnqgialm iekyqhgdfg ycprvycesq qmlpiglsdv
      121 pgevmvklyc pkcmdvygpk sslhhhtdga yfgtgfphml fmvhpeyrpk sptnkfvprl
      181 ygfkihplay qqeqqeaasf kapvramnyg ngkr
//
LOCUS       XP_077485200             136 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095293 [Amblyomma americanum].
ACCESSION   XP_077485200
VERSION     XP_077485200.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629074.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..136
                     /product="uncharacterized protein LOC144095293"
                     /calculated_mol_wt=15125
     CDS             1..136
                     /gene="LOC144095293"
                     /coded_by="XM_077629074.1:205..615"
                     /db_xref="GeneID:144095293"
ORIGIN      
        1 mahvfvkwqe ekwdvyaira ldadvgmqll akesaieelr gimvmvewea sqppasatll
       61 gfgtkramer qrtrlaleae vgavpntpet esgtqpptkr qkgthapsfl ecrdnrldta
      121 fppwspeksn yivdsl
//
LOCUS       XP_077485213             117 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095311 [Amblyomma americanum].
ACCESSION   XP_077485213
VERSION     XP_077485213.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629087.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..117
                     /product="uncharacterized protein LOC144095311"
                     /calculated_mol_wt=12443
     CDS             1..117
                     /gene="LOC144095311"
                     /coded_by="XM_077629087.1:193..546"
                     /db_xref="GeneID:144095311"
ORIGIN      
        1 mapaaavspa pspapqapea mdehdycsvq enedqtgara appptqaaph qqggsirrph
       61 alpprqqdhp gsvqqrhnai atlieqqgrl atiqdqqlrq mttdlahira hlagvkh
//
LOCUS       XP_077485229             423 aa            linear   INV 25-NOV-2025
DEFINITION  chromosome-associated kinesin KIF4A-like [Amblyomma americanum].
ACCESSION   XP_077485229
VERSION     XP_077485229.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629103.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..423
                     /product="chromosome-associated kinesin KIF4A-like"
                     /calculated_mol_wt=46595
     Region          <10..240
                     /region_name="KISc"
                     /note="Kinesin motor, catalytic domain. ATPase;
                     smart00129"
                     /db_xref="CDD:214526"
     Region          <235..>412
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..423
                     /gene="LOC144095335"
                     /coded_by="XM_077629103.1:44..1315"
                     /db_xref="GeneID:144095335"
ORIGIN      
        1 mgircsknad degiipraig difknmtttt gksflvrvsf leiyqeqifd llsnsrgrvp
       61 lairedktgt vkvpnltqhk vatadetlrl vgigsasrit aatnmnicss rshailtltv
      121 eqqnesggat tvakmnfvdl agseraektk aigerfkegm kinhgllslg nvvsslserk
      181 fhvpyrnskl trllqdslgg sshtvmiaci spasgnredt ldtlryadra rkiknkptan
      241 idptqaeian lrqklqeaea almrctthev qnpiasggae daellkdkle lalahnaqle
      301 tenrrlaakl qqaqdqarkq seqvispmek tvsklgeeha paecmsrike leakvqrler
      361 gferkilevk kelqelslfk hsansttesh qdicaslveg legqaqeiyk lqnaqdepkk
      421 kte
//
LOCUS       XP_077485259             423 aa            linear   INV 25-NOV-2025
DEFINITION  chromosome-associated kinesin KIF4A-like [Amblyomma americanum].
ACCESSION   XP_077485259
VERSION     XP_077485259.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629133.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..423
                     /product="chromosome-associated kinesin KIF4A-like"
                     /calculated_mol_wt=46643
     Region          <10..240
                     /region_name="KISc"
                     /note="Kinesin motor, catalytic domain. ATPase;
                     smart00129"
                     /db_xref="CDD:214526"
     Region          <244..>412
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..423
                     /gene="LOC144095368"
                     /coded_by="XM_077629133.1:44..1315"
                     /db_xref="GeneID:144095368"
ORIGIN      
        1 mgircsknad degiipraig difknmtttt gksflvrvsf leiyqeqifd llsnsrgrvp
       61 lairedktgt vkvpnlterk vatadetlrl vgigsasrit aatnmnicss rshailtltv
      121 eqqnesggat tvakmnfvdl agseraektk aigerfkegm kinhgllslg nvvsslserk
      181 fhvpyrnskl trllqdslgg sshtvmiaci spasgnredt ldtlryadra rkiknkptvn
      241 idptqaeian lrqklqeaea almrctthev qnpiasggae daellkdkle lalahnaqle
      301 tenrrlaakl qqaqdqarkq seqvispmek tvsklgeeha paecmsrike leakvqrler
      361 gferkilevk kelqelslfk hsansttesh qdicaslveg legqaqeiyk lqnaqdepkk
      421 kte
//
LOCUS       XP_077485267             423 aa            linear   INV 25-NOV-2025
DEFINITION  chromosome-associated kinesin KIF4A-like [Amblyomma americanum].
ACCESSION   XP_077485267
VERSION     XP_077485267.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629141.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..423
                     /product="chromosome-associated kinesin KIF4A-like"
                     /calculated_mol_wt=46615
     Region          <10..240
                     /region_name="KISc"
                     /note="Kinesin motor, catalytic domain. ATPase;
                     smart00129"
                     /db_xref="CDD:214526"
     Region          <235..>412
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..423
                     /gene="LOC144095382"
                     /coded_by="XM_077629141.1:44..1315"
                     /db_xref="GeneID:144095382"
ORIGIN      
        1 mgircsknad degiipraig difknmtttt gksflvrvsf leiyqeqifd llsnsrgrvp
       61 lairedktgt vkvpnlterk vatadetlrl vgigsasrit aatnmnicss rshailtltv
      121 eqqnesggat tvakmnfvdl agseraektk aigerfkegm kinhgllslg nvvsslserk
      181 fhvpyrnskl trllqdslgg sshtvmiaci spasgnredt ldtlryadra rkiknkptan
      241 idptqaeian lrqklqeaea almrctthev qnpiasggae daellkdkle lalahnaqle
      301 tenrrlaakl qqaqdqarkq seqvispmek tvsklgeeha paecmsrike leakvqrler
      361 gferkilevk kelqelslfk hsansttesh qdicaslveg legqaqeiyk lqnaqdepkk
      421 kte
//
LOCUS       XP_077485279             423 aa            linear   INV 25-NOV-2025
DEFINITION  chromosome-associated kinesin KIF4A-like [Amblyomma americanum].
ACCESSION   XP_077485279
VERSION     XP_077485279.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629153.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..423
                     /product="chromosome-associated kinesin KIF4A-like"
                     /calculated_mol_wt=46595
     Region          <10..240
                     /region_name="KISc"
                     /note="Kinesin motor, catalytic domain. ATPase;
                     smart00129"
                     /db_xref="CDD:214526"
     Region          <235..>412
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..423
                     /gene="LOC144095397"
                     /coded_by="XM_077629153.1:44..1315"
                     /db_xref="GeneID:144095397"
ORIGIN      
        1 mgircsknad degiipraig difknmtttt gksflvrvsf leiyqeqifd llsnsrgrvp
       61 lairedktgt vkvpnltqhk vatadetlrl vgigsasrit aatnmnicss rshailtltv
      121 eqqnesggat tvakmnfvdl agseraektk aigerfkegm kinhgllslg nvvsslserk
      181 fhvpyrnskl trllqdslgg sshtvmiaci spasgnredt ldtlryadra rkiknkptan
      241 idptqaeian lrqklqeaea almrctthev qnpiasggae daellkdkle lalahnaqle
      301 tenrrlaakl qqaqdqarkq seqvispmek tvsklgeeha paecmsrike leakvqrler
      361 gferkilevk kelqelslfk hsansttesh qdicaslveg legqaqeiyk lqnaqdepkk
      421 kte
//
LOCUS       XP_077485294             126 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095424 [Amblyomma americanum].
ACCESSION   XP_077485294
VERSION     XP_077485294.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629168.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..126
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..126
                     /product="uncharacterized protein LOC144095424"
                     /calculated_mol_wt=13834
     CDS             1..126
                     /gene="LOC144095424"
                     /coded_by="XM_077629168.1:625..1005"
                     /db_xref="GeneID:144095424"
ORIGIN      
        1 mcilavdsrs pgsfrdafvw rhseaeegll ddgdffsgrl sthwshgclh qlhditdltq
       61 qkacttrhml shnlwlsavl vcrraasvaf kdtgctsasg sgqcassphv lhftasvwmc
      121 pyqqrv
//
LOCUS       XP_077485310             759 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095444 [Amblyomma americanum].
ACCESSION   XP_077485310
VERSION     XP_077485310.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629184.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..759
                     /product="uncharacterized protein LOC144095444"
                     /calculated_mol_wt=83847
     CDS             1..759
                     /gene="LOC144095444"
                     /coded_by="XM_077629184.1:162..2441"
                     /db_xref="GeneID:144095444"
ORIGIN      
        1 makssassfg rglsaaqrme dfanylyseg pdafqnvyrv rrfldladhr frgsvgrvrs
       61 ffspcthgll etdcwalafh erfnavlnpr gmemllaheg syafqtieka tdilmgtgdv
      121 lsmciclwll kehrciskvv vdvavvagyh selfwkllsl nkahldvvel rglpnvpvgf
      181 kiyplfknan rltaltlvsl hlnreevvkl cdlislnagl trlvlqdlgi dplgvgrish
      241 aileheqpkv lvlrdkasrh rgfedvvsgl ldtklqklil nlpcrlsclf drlgcntslt
      301 elevvdttpr lhlslhdlat slmtnttlsr ltlrifvpea nmdflggwqr ltrsiacnqs
      361 lkylsfatsr fgvresacms algdaiglnk tlqelsvqgc rlncasllyl lgglvqnktl
      421 qelklgdlga ddrtseqilq qvidlrlger vdcvytlrhg kvlekvctpn ealsirglrl
      481 acaqvddrda mlaclsllre slttlrvegv pmtdegaycl aklfaeskvi rdvmllfpat
      541 adqvalmmsg laaseivniv tvgggwelyg vaaeafeevv rdnrsirelt vfqetalgye
      601 elksylqrgv tgndvivklr llhgrnrtes rdfevmqtlq rnrvvckwaa eailgnkitg
      661 atlstlsrmn dctrcrallr damgmneetv qkktiealet inaqlervds nitpntttas
      721 aepapstsrg assaaeakqt akgkgrrrka agpktkkpk
//
LOCUS       XP_077485327             447 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095470 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077485327
VERSION     XP_077485327.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629201.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..447
                     /product="uncharacterized protein LOC144095470 isoform X1"
                     /calculated_mol_wt=48114
     Region          45..>204
                     /region_name="PLN02768"
                     /note="AMP deaminase"
                     /db_xref="CDD:215411"
     CDS             1..447
                     /gene="LOC144095470"
                     /coded_by="XM_077629201.1:181..1524"
                     /db_xref="GeneID:144095470"
ORIGIN      
        1 matwndadwq neadigsaae svqvaaqnss lecdidvsvt larssyttas krtgpfvdlt
       61 sdksglslec fcsdtlpgse dsildgdvdk efppgdsali pglsdvslpp khviaadpld
      121 lslsdgddvl apqapstkgl etireepele aqmelehake rnpeelsthm eeepapleld
      181 alplrdqels tepgnevvpd psephlkvse qslaepaavq pedtvsdvtv qgrasaaaal
      241 iiedlpvrpk spyqtrnrpq isaqtpvsps spekltpppa dsrppplsqr ntlgarrktt
      301 aacteapran evippvvakg yvanqfleki gqlrgsavaa egghdtypla edlmsiegaq
      361 kqtdkykkee fgelftkrps caahliaksa veaesededs aldfdhlpir rrmdiwkkre
      421 drairkglil ipkmckqgrf aisaagl
//
LOCUS       XP_077485331             447 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095470 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077485331
VERSION     XP_077485331.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629205.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..447
                     /product="uncharacterized protein LOC144095470 isoform X1"
                     /calculated_mol_wt=48114
     Region          45..>204
                     /region_name="PLN02768"
                     /note="AMP deaminase"
                     /db_xref="CDD:215411"
     CDS             1..447
                     /gene="LOC144095470"
                     /coded_by="XM_077629205.1:141..1484"
                     /db_xref="GeneID:144095470"
ORIGIN      
        1 matwndadwq neadigsaae svqvaaqnss lecdidvsvt larssyttas krtgpfvdlt
       61 sdksglslec fcsdtlpgse dsildgdvdk efppgdsali pglsdvslpp khviaadpld
      121 lslsdgddvl apqapstkgl etireepele aqmelehake rnpeelsthm eeepapleld
      181 alplrdqels tepgnevvpd psephlkvse qslaepaavq pedtvsdvtv qgrasaaaal
      241 iiedlpvrpk spyqtrnrpq isaqtpvsps spekltpppa dsrppplsqr ntlgarrktt
      301 aacteapran evippvvakg yvanqfleki gqlrgsavaa egghdtypla edlmsiegaq
      361 kqtdkykkee fgelftkrps caahliaksa veaesededs aldfdhlpir rrmdiwkkre
      421 drairkglil ipkmckqgrf aisaagl
//
LOCUS       XP_077485334             437 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095470 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077485334
VERSION     XP_077485334.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629208.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..437
                     /product="uncharacterized protein LOC144095470 isoform X2"
                     /calculated_mol_wt=47089
     Region          45..>194
                     /region_name="PLN02768"
                     /note="AMP deaminase"
                     /db_xref="CDD:215411"
     CDS             1..437
                     /gene="LOC144095470"
                     /coded_by="XM_077629208.1:181..1494"
                     /db_xref="GeneID:144095470"
ORIGIN      
        1 matwndadwq neadigsaae svqvaaqnss lecdidvsvt larssyttas krtgpfvdlt
       61 sdksglslde dsildgdvdk efppgdsali pglsdvslpp khviaadpld lslsdgddvl
      121 apqapstkgl etireepele aqmelehake rnpeelsthm eeepapleld alplrdqels
      181 tepgnevvpd psephlkvse qslaepaavq pedtvsdvtv qgrasaaaal iiedlpvrpk
      241 spyqtrnrpq isaqtpvsps spekltpppa dsrppplsqr ntlgarrktt aacteapran
      301 evippvvakg yvanqfleki gqlrgsavaa egghdtypla edlmsiegaq kqtdkykkee
      361 fgelftkrps caahliaksa veaesededs aldfdhlpir rrmdiwkkre drairkglil
      421 ipkmckqgrf aisaagl
//
LOCUS       XP_077485338             137 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095484 [Amblyomma americanum].
ACCESSION   XP_077485338
VERSION     XP_077485338.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629212.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..137
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..137
                     /product="uncharacterized protein LOC144095484"
                     /calculated_mol_wt=15572
     CDS             1..137
                     /gene="LOC144095484"
                     /coded_by="XM_077629212.1:142..555"
                     /db_xref="GeneID:144095484"
ORIGIN      
        1 mstrkarrft rrqsrrqlda igsnvmrryk gcthllcylr rvrgpsakyw lsklqrlvel
       61 icwmvlirlm ivlsvltsvv vgclllasla mggpallqaq seflgrafce gvalvyhyis
      121 npqqarlldh arrgkrl
//
LOCUS       XP_077485344             714 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095493 [Amblyomma americanum].
ACCESSION   XP_077485344
VERSION     XP_077485344.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629218.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..714
                     /product="uncharacterized protein LOC144095493"
                     /calculated_mol_wt=78570
     Region          39..83
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Region          574..713
                     /region_name="MATH"
                     /note="MATH (meprin and TRAF-C homology) domain; an
                     independent folding unit with an eight-stranded
                     beta-sandwich structure found in meprins, TRAFs and other
                     proteins. Meprins comprise a class of extracellular
                     metalloproteases which are anchored to the membrane...;
                     cl02446"
                     /db_xref="CDD:445786"
     Site            order(628,684..686)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:239745"
     CDS             1..714
                     /gene="LOC144095493"
                     /coded_by="XM_077629218.1:188..2332"
                     /db_xref="GeneID:144095493"
ORIGIN      
        1 massaaekig ttytlygfgn dldwrqtcfl eplppsrycs ycgrvcatmm mlpcshivca
       61 gcygqctrsy acaldaqrfq qhdvavmiys sqdlaskhiq cwnadrgcki sglsfqiveh
      121 yhkqcahhpi kcprcnqavs hgevvthlas scsqkpaall gvqdtlkhln padtrrpgaa
      181 gpfplipdqh anlevgnvas sstaftlasq qhrttstslv lpsprqpepl cdyrgqpppa
      241 hdiyepvsry lreapaahae lpenpscdtq rrdsmarrka pasmeleimg rlehlsepks
      301 dftlesiqmq gtsphsasss pettrpgryr yldtaatlga rtlstpklng gkgaipeanv
      361 snaatvqmdh ayiptetavr tqdtgdgykr eeynssqdqp alpekpllpy dkdksdaklt
      421 sctgndatqa aplvvsqqgt satsapsdvn nedlpslver yvnsvcrfvs tnfdetdiak
      481 nalcklmped lifvlktsee iraqmseegt alsptrgtvg ealknlfqaa rehniegckd
      541 vfsqeyvaqk idnlinlakl vlsqhklvsm pvqwylhnws elkaralaag eaadyfhnis
      601 atfygytivp glllenttgt lrlhfsfylq rgvydsflew plkkeltlsi vhptdkgkvr
      661 flvvdtkkgq vegctkpetq egkpisraks ikadyfdtng yvdgnklmlk fevk
//
LOCUS       XP_077485368             201 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger RNA-binding protein-like [Amblyomma americanum].
ACCESSION   XP_077485368
VERSION     XP_077485368.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629242.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..201
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..201
                     /product="zinc finger RNA-binding protein-like"
                     /calculated_mol_wt=21529
     Region          5..35
                     /region_name="ZnF_U1"
                     /note="U1-like zinc finger; smart00451"
                     /db_xref="CDD:197732"
     Region          8..30
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Site            order(15..17,19..20,24,29,85,88..92,94..95)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275371"
     Region          78..102
                     /region_name="zf-met"
                     /note="Zinc-finger of C2H2 type; pfam12874"
                     /db_xref="CDD:463736"
     Region          80..102
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     CDS             1..201
                     /gene="LOC144095546"
                     /coded_by="XM_077629242.1:93..698"
                     /db_xref="GeneID:144095546"
ORIGIN      
        1 masmgyfcql csmqlngpap ykdhlkgakh lkkvathgnl ealseggrpa aesamdtdas
       61 aasvgsptil aqnplfpfvc klcnvamnce aalvshntgk khmkalytee tmrqvslygt
      121 ifagpvpepa pnqtamspta paslppqdqp meheddvdls chycgillfk siqyklehle
      181 tdahcnkkmq vigqlggtlq e
//
LOCUS       XP_077485374             215 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095553 [Amblyomma americanum].
ACCESSION   XP_077485374
VERSION     XP_077485374.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629248.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..215
                     /product="uncharacterized protein LOC144095553"
                     /calculated_mol_wt=23103
     Region          5..35
                     /region_name="zf-U1"
                     /note="U1 zinc finger; cl22907"
                     /db_xref="CDD:389966"
     Region          77..108
                     /region_name="ZnF_U1"
                     /note="U1-like zinc finger; smart00451"
                     /db_xref="CDD:197732"
     CDS             1..215
                     /gene="LOC144095553"
                     /coded_by="XM_077629248.1:140..787"
                     /db_xref="GeneID:144095553"
ORIGIN      
        1 masmgyscql csmhfsgpvp cmdhlksakh qkkvateryl ealaeggrpa agsamnthvs
       61 aasagsatil aqnallpfvc klcnvamncq evlivhnsgk khqkalqree vqrqislcyg
      121 iralsssesa pnqstmspta passpqqdqp lehgedvdls chycgiilfk sieyklehle
      181 tdahcnrklq gigrlggtle qspivlnvvq vikhp
//
LOCUS       XP_077485384              55 aa            linear   INV 25-NOV-2025
DEFINITION  kinesin-II 95 kDa subunit-like [Amblyomma americanum].
ACCESSION   XP_077485384
VERSION     XP_077485384.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629258.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..55
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..55
                     /product="kinesin-II 95 kDa subunit-like"
                     /calculated_mol_wt=5895
     Region          <6..>53
                     /region_name="Motor_domain"
                     /note="Myosin and Kinesin motor domain; cl22853"
                     /db_xref="CDD:473979"
     CDS             1..55
                     /gene="LOC144095565"
                     /coded_by="XM_077629258.1:11..178"
                     /db_xref="GeneID:144095565"
ORIGIN      
        1 mgircssnad dagiipraig difkniattt gksflvrvsf leiyqeqisd flsks
//
LOCUS       XP_077485396             215 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095591 [Amblyomma americanum].
ACCESSION   XP_077485396
VERSION     XP_077485396.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629270.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..215
                     /product="uncharacterized protein LOC144095591"
                     /calculated_mol_wt=24012
     Region          136..>205
                     /region_name="UBCc_UEV"
                     /note="Ubiquitin-conjugating enzyme E2, catalytic (UBCc)
                     domain/ubiquitin E2 variant (UEV) domain; cl49610"
                     /db_xref="CDD:483950"
     CDS             1..215
                     /gene="LOC144095591"
                     /coded_by="XM_077629270.1:71..718"
                     /db_xref="GeneID:144095591"
ORIGIN      
        1 mprctnsqhh gevqnalqey irrssgifdl rirerpcmlw ssaaeptiad mtgqrkqgfa
       61 vvpgerrdln ilvrivllgg styksgffrq lvkcmvffaa gprgehqgvp kyrtgrwsla
      121 flravvdtie hrkqgvepde rgvtkiqahn velagapiwg gffhfllkfi tcesqavaaa
      181 ervrvvpnsh eigkarlvll stwngrcrlp ytgai
//
LOCUS       XP_077485403             137 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095599 [Amblyomma americanum].
ACCESSION   XP_077485403
VERSION     XP_077485403.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629277.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..137
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..137
                     /product="uncharacterized protein LOC144095599"
                     /calculated_mol_wt=13816
     CDS             1..137
                     /gene="LOC144095599"
                     /coded_by="XM_077629277.1:90..503"
                     /db_xref="GeneID:144095599"
ORIGIN      
        1 madkgpaagv amelavfaan hgwtskgegs adrqaehqls sgttqrhgqd paggddllta
       61 vvvgagpglr ddgasenegi paaarataac kqpplcgglk gpltfclaas lafvvvligl
      121 vayiyrtryr easpenl
//
LOCUS       XP_077485412             224 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095612 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077485412
VERSION     XP_077485412.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629286.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..224
                     /product="uncharacterized protein LOC144095612 isoform X1"
                     /calculated_mol_wt=22846
     Region          45..122
                     /region_name="HFD_H2A"
                     /note="histone-fold domain found in histone H2A and
                     similar proteins; cd00074"
                     /db_xref="CDD:467020"
     Site            order(45..46,57..58,61,65,111,113..115,118)
                     /site_type="other"
                     /note="Hif1 binding site [polypeptide binding]"
                     /db_xref="CDD:467020"
     Site            order(45..46,49,57..58,61,71..74,103,109,111)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:467020"
     Site            order(46..48,53..55,58..59,61..63,71,73,75..76,78..93,
                     95..97,99,109,111..114,117,121)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467020"
     Site            order(67,70)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:467020"
     Region          <158..205
                     /region_name="RPA_2b-aaRSs_OBF_like"
                     /note="Replication protein A, class 2b aminoacyl-tRNA
                     synthetases, and related proteins with
                     oligonucleotide/oligosaccharide (OB) fold; cl09930"
                     /db_xref="CDD:471953"
     CDS             1..224
                     /gene="LOC144095612"
                     /coded_by="XM_077629286.1:100..774"
                     /db_xref="GeneID:144095612"
ORIGIN      
        1 mlrstpddei afffipistp tpthipamas lefkaessae ptamsrsara glrlpvgrih
       61 rlmcngkyae rigstaavym aavleyltae llqlasngar dngardnakt limpchiqla
      121 irshdlrglr rklrrkmrlk asqrdgamap sgdaitpggg gdtmtpgggg damtpggggd
      181 amtpggggda ttsggggdat asgggvlpyi ptehppkesg asss
//
LOCUS       XP_077485417             223 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095612 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077485417
VERSION     XP_077485417.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629291.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..223
                     /product="uncharacterized protein LOC144095612 isoform X2"
                     /calculated_mol_wt=22759
     Region          44..121
                     /region_name="HFD_H2A"
                     /note="histone-fold domain found in histone H2A and
                     similar proteins; cd00074"
                     /db_xref="CDD:467020"
     Site            order(44..45,56..57,60,64,110,112..114,117)
                     /site_type="other"
                     /note="Hif1 binding site [polypeptide binding]"
                     /db_xref="CDD:467020"
     Site            order(44..45,48,56..57,60,70..73,102,108,110)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:467020"
     Site            order(45..47,52..54,57..58,60..62,70,72,74..75,77..92,
                     94..96,98,108,110..113,116,120)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467020"
     Site            order(66,69)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:467020"
     Region          <157..204
                     /region_name="RPA_2b-aaRSs_OBF_like"
                     /note="Replication protein A, class 2b aminoacyl-tRNA
                     synthetases, and related proteins with
                     oligonucleotide/oligosaccharide (OB) fold; cl09930"
                     /db_xref="CDD:471953"
     CDS             1..223
                     /gene="LOC144095612"
                     /coded_by="XM_077629291.1:100..771"
                     /db_xref="GeneID:144095612"
ORIGIN      
        1 mlrstpddei afffipitpt pthipamasl efkaessaep tamsrsarag lrlpvgrihr
       61 lmcngkyaer igstaavyma avleyltael lqlasngard ngardnaktl impchiqlai
      121 rshdlrglrr klrrkmrlka sqrdgamaps gdaitpgggg dtmtpggggd amtpggggda
      181 mtpggggdat tsggggdata sgggvlpyip tehppkesga sss
//
LOCUS       XP_077485426             224 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095626 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077485426
VERSION     XP_077485426.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629300.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..224
                     /product="uncharacterized protein LOC144095626 isoform X1"
                     /calculated_mol_wt=22945
     Region          45..122
                     /region_name="HFD_H2A"
                     /note="histone-fold domain found in histone H2A and
                     similar proteins; cd00074"
                     /db_xref="CDD:467020"
     Site            order(45..46,57..58,61,65,111,113..115,118)
                     /site_type="other"
                     /note="Hif1 binding site [polypeptide binding]"
                     /db_xref="CDD:467020"
     Site            order(45..46,49,57..58,61,71..74,103,109,111)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:467020"
     Site            order(46..48,53..55,58..59,61..63,71,73,75..76,78..93,
                     95..97,99,109,111..114,117,121)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467020"
     Site            order(67,70)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:467020"
     Region          <158..205
                     /region_name="RPA_2b-aaRSs_OBF_like"
                     /note="Replication protein A, class 2b aminoacyl-tRNA
                     synthetases, and related proteins with
                     oligonucleotide/oligosaccharide (OB) fold; cl09930"
                     /db_xref="CDD:471953"
     CDS             1..224
                     /gene="LOC144095626"
                     /coded_by="XM_077629300.1:37..711"
                     /db_xref="GeneID:144095626"
ORIGIN      
        1 mlrstpddei afffipistp tpthipamas lefkaessae ptamsrsara glrlpvgrih
       61 rlmcngkyae rigstaavym aavleyltae llqlasngar dngardnakt limpchiqla
      121 irshdlrrlr rklrrkmrlk asqrdgamap sgdaitpggg gdtmtpgggg damtpggggd
      181 amtpggggda ttsggggdat asgggvlpyi ptehppkesg asss
//
LOCUS       XP_077485432             223 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095626 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077485432
VERSION     XP_077485432.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629306.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..223
                     /product="uncharacterized protein LOC144095626 isoform X2"
                     /calculated_mol_wt=22858
     Region          44..121
                     /region_name="HFD_H2A"
                     /note="histone-fold domain found in histone H2A and
                     similar proteins; cd00074"
                     /db_xref="CDD:467020"
     Site            order(44..45,56..57,60,64,110,112..114,117)
                     /site_type="other"
                     /note="Hif1 binding site [polypeptide binding]"
                     /db_xref="CDD:467020"
     Site            order(44..45,48,56..57,60,70..73,102,108,110)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:467020"
     Site            order(45..47,52..54,57..58,60..62,70,72,74..75,77..92,
                     94..96,98,108,110..113,116,120)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467020"
     Site            order(66,69)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:467020"
     Region          <157..204
                     /region_name="RPA_2b-aaRSs_OBF_like"
                     /note="Replication protein A, class 2b aminoacyl-tRNA
                     synthetases, and related proteins with
                     oligonucleotide/oligosaccharide (OB) fold; cl09930"
                     /db_xref="CDD:471953"
     CDS             1..223
                     /gene="LOC144095626"
                     /coded_by="XM_077629306.1:37..708"
                     /db_xref="GeneID:144095626"
ORIGIN      
        1 mlrstpddei afffipitpt pthipamasl efkaessaep tamsrsarag lrlpvgrihr
       61 lmcngkyaer igstaavyma avleyltael lqlasngard ngardnaktl impchiqlai
      121 rshdlrrlrr klrrkmrlka sqrdgamaps gdaitpgggg dtmtpggggd amtpggggda
      181 mtpggggdat tsggggdata sgggvlpyip tehppkesga sss
//
LOCUS       XP_077485437             224 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095640 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077485437
VERSION     XP_077485437.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629311.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..224
                     /product="uncharacterized protein LOC144095640 isoform X1"
                     /calculated_mol_wt=22975
     Region          45..122
                     /region_name="HFD_H2A"
                     /note="histone-fold domain found in histone H2A and
                     similar proteins; cd00074"
                     /db_xref="CDD:467020"
     Site            order(45..46,57..58,61,65,111,113..115,118)
                     /site_type="other"
                     /note="Hif1 binding site [polypeptide binding]"
                     /db_xref="CDD:467020"
     Site            order(45..46,49,57..58,61,71..74,103,109,111)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:467020"
     Site            order(46..48,53..55,58..59,61..63,71,73,75..76,78..93,
                     95..97,99,109,111..114,117,121)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467020"
     Site            order(67,70)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:467020"
     Region          <158..205
                     /region_name="RPA_2b-aaRSs_OBF_like"
                     /note="Replication protein A, class 2b aminoacyl-tRNA
                     synthetases, and related proteins with
                     oligonucleotide/oligosaccharide (OB) fold; cl09930"
                     /db_xref="CDD:471953"
     CDS             1..224
                     /gene="LOC144095640"
                     /coded_by="XM_077629311.1:59..733"
                     /db_xref="GeneID:144095640"
ORIGIN      
        1 mlrstpddei afffipistp tpthipamas lefkaessae ptamsrsara glwlpvgrih
       61 rlmcngkyae rigstaavym aavleyltae llqlasngar dngardnakt limpchiqla
      121 irshdlrrlr rklrrkmrlk asqrdgamap sgdaitpggg gdtmtpgggg damtpggggd
      181 amtpggggda ttsggggdat asgggvlpyi ptehppkesg asss
//
LOCUS       XP_077485445             223 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095640 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077485445
VERSION     XP_077485445.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629319.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..223
                     /product="uncharacterized protein LOC144095640 isoform X2"
                     /calculated_mol_wt=22888
     Region          44..121
                     /region_name="HFD_H2A"
                     /note="histone-fold domain found in histone H2A and
                     similar proteins; cd00074"
                     /db_xref="CDD:467020"
     Site            order(44..45,56..57,60,64,110,112..114,117)
                     /site_type="other"
                     /note="Hif1 binding site [polypeptide binding]"
                     /db_xref="CDD:467020"
     Site            order(44..45,48,56..57,60,70..73,102,108,110)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:467020"
     Site            order(45..47,52..54,57..58,60..62,70,72,74..75,77..92,
                     94..96,98,108,110..113,116,120)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467020"
     Site            order(66,69)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:467020"
     Region          <157..204
                     /region_name="RPA_2b-aaRSs_OBF_like"
                     /note="Replication protein A, class 2b aminoacyl-tRNA
                     synthetases, and related proteins with
                     oligonucleotide/oligosaccharide (OB) fold; cl09930"
                     /db_xref="CDD:471953"
     CDS             1..223
                     /gene="LOC144095640"
                     /coded_by="XM_077629319.1:59..730"
                     /db_xref="GeneID:144095640"
ORIGIN      
        1 mlrstpddei afffipitpt pthipamasl efkaessaep tamsrsarag lwlpvgrihr
       61 lmcngkyaer igstaavyma avleyltael lqlasngard ngardnaktl impchiqlai
      121 rshdlrrlrr klrrkmrlka sqrdgamaps gdaitpgggg dtmtpggggd amtpggggda
      181 mtpggggdat tsggggdata sgggvlpyip tehppkesga sss
//
LOCUS       XP_077485454             197 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095656 [Amblyomma americanum].
ACCESSION   XP_077485454
VERSION     XP_077485454.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629328.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..197
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..197
                     /product="uncharacterized protein LOC144095656"
                     /calculated_mol_wt=20008
     Region          18..95
                     /region_name="HFD_H2A"
                     /note="histone-fold domain found in histone H2A and
                     similar proteins; cd00074"
                     /db_xref="CDD:467020"
     Site            order(18..19,30..31,34,38,84,86..88,91)
                     /site_type="other"
                     /note="Hif1 binding site [polypeptide binding]"
                     /db_xref="CDD:467020"
     Site            order(18..19,22,30..31,34,44..47,76,82,84)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:467020"
     Site            order(19..21,26..28,31..32,34..36,44,46,48..49,51..66,
                     68..70,72,82,84..87,90,94)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467020"
     Site            order(40,43)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:467020"
     CDS             1..197
                     /gene="LOC144095656"
                     /coded_by="XM_077629328.1:82..675"
                     /db_xref="GeneID:144095656"
ORIGIN      
        1 maslefkaes saeptamsrs araglrlpvg rihrlmcngk yaerigstaa vymaavleyl
       61 ttellqlasn gardngardn aktlimpchi qlairshdlr rlrrklrrkm rlkasqrdga
      121 mtpsgdaitp ggggdtmtpg gggdamtpgg ggdamtpggg gdattsgggg datasgggvl
      181 pyiptehppk esgasss
//
LOCUS       XP_077485462             197 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095656 [Amblyomma americanum].
ACCESSION   XP_077485462
VERSION     XP_077485462.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629336.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..197
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..197
                     /product="uncharacterized protein LOC144095656"
                     /calculated_mol_wt=20008
     Region          18..95
                     /region_name="HFD_H2A"
                     /note="histone-fold domain found in histone H2A and
                     similar proteins; cd00074"
                     /db_xref="CDD:467020"
     Site            order(18..19,30..31,34,38,84,86..88,91)
                     /site_type="other"
                     /note="Hif1 binding site [polypeptide binding]"
                     /db_xref="CDD:467020"
     Site            order(18..19,22,30..31,34,44..47,76,82,84)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:467020"
     Site            order(19..21,26..28,31..32,34..36,44,46,48..49,51..66,
                     68..70,72,82,84..87,90,94)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467020"
     Site            order(40,43)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:467020"
     CDS             1..197
                     /gene="LOC144095656"
                     /coded_by="XM_077629336.1:71..664"
                     /db_xref="GeneID:144095656"
ORIGIN      
        1 maslefkaes saeptamsrs araglrlpvg rihrlmcngk yaerigstaa vymaavleyl
       61 ttellqlasn gardngardn aktlimpchi qlairshdlr rlrrklrrkm rlkasqrdga
      121 mtpsgdaitp ggggdtmtpg gggdamtpgg ggdamtpggg gdattsgggg datasgggvl
      181 pyiptehppk esgasss
//
LOCUS       XP_077485466             333 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095672 [Amblyomma americanum].
ACCESSION   XP_077485466
VERSION     XP_077485466.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629340.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..333
                     /product="uncharacterized protein LOC144095672"
                     /calculated_mol_wt=36015
     Region          <1..302
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     CDS             1..333
                     /gene="LOC144095672"
                     /coded_by="XM_077629340.1:14..1015"
                     /db_xref="GeneID:144095672"
ORIGIN      
        1 mllwsltrer kddflksgev raelqvrrgk spenlweegl dalsakqgem eraerneera
       61 akaakgfkgr rgkvgtfkkt etetkpsalg ecvvskiewe aekidkgtgp hrgarkadhd
      121 dhddhdsgas aecspddpgq lvlskapqrn raasaeteps gskpkpvkrk nswsgssese
      181 gvpmtsgsds dkvypdvpaa gpskrqpprq qqlehkkrgp patsqrqmaa ddnvdeearg
      241 ddedfkpsge vpsrkppmal raakkttkev aadsdsdaap lppkapknir amlsssddee
      301 gspavpmppq rpaggrsraa vkydfgsdse eef
//
LOCUS       XP_077485472             442 aa            linear   INV 25-NOV-2025
DEFINITION  neuropeptide SIFamide receptor-like [Amblyomma americanum].
ACCESSION   XP_077485472
VERSION     XP_077485472.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629346.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..442
                     /product="neuropeptide SIFamide receptor-like"
                     /calculated_mol_wt=48237
     Region          91..382
                     /region_name="7tmA_CCKR-like"
                     /note="cholecystokinin receptors and related proteins,
                     member of the class A family of seven-transmembrane G
                     protein-coupled receptors; cd14993"
                     /db_xref="CDD:320124"
     Region          91..116
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320124"
     Region          123..150
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320124"
     Site            order(146,149..150,162..167,169..170,173,216,218..222,252,
                     255..256,258,260..262,264..265,323,326..327,329..330,333,
                     349..350,352..354,357,360..361)
                     /site_type="other"
                     /note="putative peptide ligand binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:320124"
     Region          162..192
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320124"
     Region          204..222
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320124"
     Region          252..278
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320124"
     Region          303..333
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320124"
     Region          350..375
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320124"
     CDS             1..442
                     /gene="LOC144095679"
                     /coded_by="XM_077629346.1:220..1548"
                     /db_xref="GeneID:144095679"
ORIGIN      
        1 mrqanvtfhc yglyhwtlst lvpalsgppl vaagpngtgg glamlataaa pqappstgaa
       61 tgdaaagael steewvdacf lrhsapwvvg ycvaytavfv vgvvgnccvl rvvsrnasmk
      121 nsafhallvn lavadlmviv fclpvtlvgh lfgpwllglf vcksvsylqg vsvcasvntl
      181 maisidryya icypmkrqis lracrvviav iwafsltitl pwtfffrlmp mltesettlq
      241 vcredwpteg mgvlyfvvan lvlcyllplc vitlcyvfiw lkvwrrrppg eahdfaveni
      301 iqrskikvak mllvvvvvfa iswlplyaif arlkigepla dgsleqslie vaapvaqwlg
      361 asnscinpil yaffnskfrm gfksilfrrq cccwcccdga sggrrrsaas aarhelscnr
      421 pssvrtsqav arrkdsrlsd vf
//
LOCUS       XP_077485483             433 aa            linear   INV 25-NOV-2025
DEFINITION  QRFP-like peptide receptor [Amblyomma americanum].
ACCESSION   XP_077485483
VERSION     XP_077485483.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629357.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..433
                     /product="QRFP-like peptide receptor"
                     /calculated_mol_wt=47517
     Region          48..351
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          50..74
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410628"
     Region          83..105
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410628"
     Region          121..143
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410628"
     Region          164..180
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410628"
     Region          224..247
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410628"
     Region          278..300
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410628"
     Region          319..344
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410628"
     CDS             1..433
                     /gene="LOC144095708"
                     /coded_by="XM_077629357.1:249..1550"
                     /db_xref="GeneID:144095708"
ORIGIN      
        1 maelncsneq ahiggdvllv nlsafgdelg gssiwggngg tsftwseyik igaylttiav
       61 aavgntcvil tvalnrslrt tincyltnla vadaiismfc mwvylvknls dwyvlgafmc
      121 riegfaqmta vtcsvltlsa iscdrftail ypfraritkq rtggvlaavw llsatvaspl
      181 llyttpyyvq wsdveltycg eswpqriewd pteaacvars pfkqlyytav tvalffvpvl
      241 vmssayalil halwrdrhpg eanannallh srakrkvvkl vcvvlvvfvl cwmpfqvivl
      301 fsqfrdngvh sqplpswfsp asfwctylay ansalnpiiy ggmtptfrqg lanvlrcghr
      361 rraeypkrws vrsrltlttg pnagsaarpl rlcaktsysg tssttmllta hratppaprc
      421 acrigrvcaa tdv
//
LOCUS       XP_077485485             165 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095740 [Amblyomma americanum].
ACCESSION   XP_077485485
VERSION     XP_077485485.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629359.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..165
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..165
                     /product="uncharacterized protein LOC144095740"
                     /calculated_mol_wt=18992
     Region          <26..76
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..165
                     /gene="LOC144095740"
                     /coded_by="XM_077629359.1:319..816"
                     /db_xref="GeneID:144095740"
ORIGIN      
        1 mrpyarrdlq khrlrpsgre eyqqratfny rlsrarrvik ksfgimaarw rilrrpfras
       61 eettenicka cvvlhnfmms esalarsays ppgyadsedw qgnetpglwr tdgndlpamr
      121 gisrqgchsa rsamdvrdrl aqyfvtdgkv pwqeaivnra grkea
//
LOCUS       XP_077485486             321 aa            linear   INV 25-NOV-2025
DEFINITION  WD repeat-containing protein 54-like [Amblyomma americanum].
ACCESSION   XP_077485486
VERSION     XP_077485486.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629360.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..321
                     /product="WD repeat-containing protein 54-like"
                     /calculated_mol_wt=34526
     Region          <130..314
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          166..200
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          206..240
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          246..281
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..321
                     /gene="LOC144095843"
                     /coded_by="XM_077629360.1:1..966"
                     /db_xref="GeneID:144095843"
ORIGIN      
        1 myqkdknllc rhsasplpnn ltacteggkv nlafvgktev tallrspdgs vhqmhvackd
       61 vggspcnelt qvkwvklddi ktllviastr afqvfewdgs vflhchplpq eldateetft
      121 rgigscgpsl lcvgtnsgti lvhlvsrdlt vtfcqktteh aphavadihs hgeimasadd
      181 gggiclwrgk talslvrklp pagspcvsvr vwhdlvlagy gtgelrvfst rsfqimaeat
      241 aharwitaid vapdtglals vaedsyvrvw qldregehti dhvhgesvad amlmgcaflv
      301 pdgssfatva ydstevfvfk k
//
LOCUS       XP_077485487             273 aa            linear   INV 25-NOV-2025
DEFINITION  etoposide-induced protein 2.4-like [Amblyomma americanum].
ACCESSION   XP_077485487
VERSION     XP_077485487.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629361.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..273
                     /product="etoposide-induced protein 2.4-like"
                     /calculated_mol_wt=30497
     Region          81..215
                     /region_name="EI24"
                     /note="Etoposide-induced protein 2.4 (EI24); pfam07264"
                     /db_xref="CDD:462129"
     CDS             1..273
                     /gene="LOC144095859"
                     /coded_by="XM_077629361.1:11..832"
                     /db_xref="GeneID:144095859"
ORIGIN      
        1 mdalrgvsdc fkgafiveta rpiapdgapa aqpprlerrd dggpsvllnp lwrcvfsvgv
       61 fgvfglgiav fdmwtwpqll lvfesealcl gllfmvvmll nnlvfqdiad lafdrtagsq
      121 psilksltrm ladtvsnvlm hmsfllqahl fgmlpvgslt ifflvhlsll cslnafdykw
      181 rslgwglaqr irfvednwiy fvgfgmplaf ftwlpssffl rgvififsfp flivaayatt
      241 rptgparftl plftpfiwls ncinqrltee vfa
//
LOCUS       XP_077485488             293 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095875 [Amblyomma americanum].
ACCESSION   XP_077485488
VERSION     XP_077485488.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629362.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..293
                     /product="uncharacterized protein LOC144095875"
                     /calculated_mol_wt=33786
     Region          <46..247
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..293
                     /gene="LOC144095875"
                     /coded_by="XM_077629362.1:1..882"
                     /db_xref="GeneID:144095875"
ORIGIN      
        1 mverithstr nfvtslhnpi eeselrmmdk iatlrllpyt faalhdvaev drlythvpdq
       61 kdviyldnyl slcrittkym vsllldttan tskffvppfr tglpymvsvy nvihlgldtv
      121 vppldksysl nyavighkva qqasqlflyg qdifnetgsy ervwsydffn alrkkyqclr
      181 rqyldqrwnt geisrrilea avtadilfna ykgvrkapqn arrfrqereq fgltpeqlff
      241 vglcinwyqr nhghrglaer acrfslpsls gfsdaficpq tappppspde cay
//
LOCUS       XP_077485490             148 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095891 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077485490
VERSION     XP_077485490.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629364.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="uncharacterized protein LOC144095891 isoform X1"
                     /calculated_mol_wt=16759
     CDS             1..148
                     /gene="LOC144095891"
                     /coded_by="XM_077629364.1:22..468"
                     /db_xref="GeneID:144095891"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedrrr rhlnrrltat mkrlsgvhvl
       61 nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar pgevyvvhrc
      121 rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077485491              98 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095891 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077485491
VERSION     XP_077485491.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629365.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..98
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..98
                     /product="uncharacterized protein LOC144095891 isoform X2"
                     /calculated_mol_wt=10604
     CDS             1..98
                     /gene="LOC144095891"
                     /coded_by="XM_077629365.1:478..774"
                     /db_xref="GeneID:144095891"
ORIGIN      
        1 mkrlsgvhvl nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar
       61 pgevyvvhrc rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077485492             149 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095949 [Amblyomma americanum].
ACCESSION   XP_077485492
VERSION     XP_077485492.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629366.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..149
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..149
                     /product="uncharacterized protein LOC144095949"
                     /calculated_mol_wt=16479
     CDS             1..149
                     /gene="LOC144095949"
                     /coded_by="XM_077629366.1:25..474"
                     /db_xref="GeneID:144095949"
ORIGIN      
        1 mkfahrlftl ailvtvpraw tqaqdpvprp aagddwlryl rpqadlsrta ldgyqrslrd
       61 leafvsqqsa tklalgppla tgdrvgapfs pflgsgdast qqtvfqqqil nntlslhell
      121 rrlfptaqpp ptrpplragp ftarrrqlr
//
LOCUS       XP_077485493             773 aa            linear   INV 25-NOV-2025
DEFINITION  N-acetylated-alpha-linked acidic dipeptidase 2-like [Amblyomma
            americanum].
ACCESSION   XP_077485493
VERSION     XP_077485493.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629367.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..773
                     /product="N-acetylated-alpha-linked acidic dipeptidase
                     2-like"
                     /calculated_mol_wt=86841
     Region          213..367
                     /region_name="PA"
                     /note="Protease-associated (PA) domain. The PA domain is
                     an insert domain in a diverse fraction of proteases. The
                     significance of the PA domain to many of the proteins in
                     which it is inserted is undetermined. It may be a
                     protein-protein interaction domain. At...; cl28883"
                     /db_xref="CDD:333703"
     Site            305..307
                     /site_type="other"
                     /note="PA/protease or protease-like domain interface
                     [polypeptide binding]"
                     /db_xref="CDD:238300"
     Region          <398..630
                     /region_name="Zinc_peptidase_like"
                     /note="Zinc peptidases M18, M20, M28, and M42; cl14876"
                     /db_xref="CDD:472712"
     Region          659..767
                     /region_name="TFR_dimer"
                     /note="Transferrin receptor-like dimerization domain;
                     pfam04253"
                     /db_xref="CDD:461238"
     CDS             1..773
                     /gene="LOC144095965"
                     /coded_by="XM_077629367.1:115..2436"
                     /db_xref="GeneID:144095965"
ORIGIN      
        1 myrtpqpvgg ygqpiplqrr gslspmamms pggpmspnmv ekvrmaaiqq rkqyartpae
       61 rlllkwvicw vlfaavattf mlwylfklse kpvvtdhmsr llsihdqist eryhqhdrvy
      121 raithnisar airnqfhnlt akdaggmdli rsvakywkdi gmdvvkepmy mvltsrpnat
      181 mdnkvmlfrd nqllltcssr dlmvlagkth ippaylafaa sgtgigtpvy vnygrhqdfg
      241 yfrnvdlhrt vvivrtgkit igekvrnaer mgaaavvvfi dasdvaprge eqpfpesiwv
      301 ggsaiqrgsa ayirgdpltp gypavdalyr tmvddaempq ipaqpisydd arsilkeldg
      361 tpcpdkwdpm lgiacfvnas apriklqvdv hnemvfrpil nvigiirgkv epdrfvmigs
      421 qthddtrgsa hpllsvtkll iqskvfchlh khhrwtprrs lmfalfyaee nslmgstewv
      481 eeymgvlqns gvlyltggml sgskfvprat pglswsirhv aelvhsneti tllqewehdk
      541 galahmavam pplgladeaa ftfgagvpsv ffhfmhrhet esmypalgta hdsyslverq
      601 vdpkfhlsrm eaqasallar lwadrtllpm dvlelaewin frvkdytnsh rsaveankls
      661 lgplqqsaeh fvesarefmn wtqkikqgnp myariyndiv vrlpkvfltp sgvptrhith
      721 khllfspegl pfpglqdlir hrpvnrnafg hmvalitesi iqatemlhfs pvv
//
LOCUS       XP_077485494             201 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase MARCHF2-like [Amblyomma americanum].
ACCESSION   XP_077485494
VERSION     XP_077485494.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629368.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..201
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..201
                     /product="E3 ubiquitin-protein ligase MARCHF2-like"
                     /calculated_mol_wt=22894
     Region          23..70
                     /region_name="RINGv"
                     /note="The RING-variant domain is a C4HC3 zinc-finger like
                     motif found in a number of cellular and viral proteins;
                     smart00744"
                     /db_xref="CDD:128983"
     Site            order(24,27,42,47,50,53,66,69)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:319725"
     CDS             1..201
                     /gene="LOC144095981"
                     /coded_by="XM_077629368.1:147..752"
                     /db_xref="GeneID:144095981"
ORIGIN      
        1 mdsmsaasag psskdsrpdd vdicricfrg vscgsllqpc mcrgsigfah kpcmerwvse
       61 rreeecnich ynypilrksr tfcellqhpe grkeplvyal lgiifslsll hvfafawllt
      121 vrmwgrlpwm yqianaaalv aqaflwsvfp fvafrmawes scrwcllnae lrillpeesd
      181 dirppragla yrarkttlar n
//
LOCUS       XP_077485495             225 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144095998 [Amblyomma americanum].
ACCESSION   XP_077485495
VERSION     XP_077485495.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629369.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..225
                     /product="uncharacterized protein LOC144095998"
                     /calculated_mol_wt=24923
     Region          <147..206
                     /region_name="IG_FLMN"
                     /note="Filamin-type immunoglobulin domains; smart00557"
                     /db_xref="CDD:214720"
     CDS             1..225
                     /gene="LOC144095998"
                     /coded_by="XM_077629369.1:104..781"
                     /db_xref="GeneID:144095998"
ORIGIN      
        1 mpwdsahlie klkkmldvsr qavregheav arlgqelldq iedntqtrlk vyrqlendsv
       61 iasgtnevql ssfskskivp qllkyevkqh pdfkasslgy iqaceavped mgfacdysam
      121 tfsvgssvrl vctctfsyyd hflfmhlavf vtspsgeevk ttnqdlenrs ymasfvptat
      181 gvhvvrvtly kqdilgsple vnvakafsle silcsprkaa aslen
//
LOCUS       XP_077485496             428 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096014 [Amblyomma americanum].
ACCESSION   XP_077485496
VERSION     XP_077485496.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629370.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..428
                     /product="uncharacterized protein LOC144096014"
                     /calculated_mol_wt=47846
     CDS             1..428
                     /gene="LOC144096014"
                     /coded_by="XM_077629370.1:94..1380"
                     /db_xref="GeneID:144096014"
ORIGIN      
        1 msmvmrkwdy fsknrhqffs tfvtsddaar ldvkdetyvp fitdnfgdwt ddiavalgsd
       61 vlrrcigypa ervrlrlykt wlanfaaqew pdtfdrdlle sivatpspsd ptkplfgdlf
      121 rqaavrprpe gtyldtsals plfkrpavld adfktakglv ayldgllsvg drealheagl
      181 iigflsllls svsavsvnrl igifrnlekf glatvlptsf sgefpcptsa nlenlrdkcg
      241 wgqsalrpyi palavaqyti fmraideapk edvlflgqsf mvhtkysgld cvdllemfak
      301 rthfpmdyln lllggnasaw trklmefyls nvaspgntqn sapwcrvvdp tffrglevgd
      361 grsyalkimc flarsqrgll yrkwsrlane tmseseveda rvwadniral lakgklrpve
      421 asrasatn
//
LOCUS       XP_077485497             428 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096030 [Amblyomma americanum].
ACCESSION   XP_077485497
VERSION     XP_077485497.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629371.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..428
                     /product="uncharacterized protein LOC144096030"
                     /calculated_mol_wt=47966
     CDS             1..428
                     /gene="LOC144096030"
                     /coded_by="XM_077629371.1:94..1380"
                     /db_xref="GeneID:144096030"
ORIGIN      
        1 msmvmrkwdy fsknrhqffs tfvtsddaar ldvkdetyvp fitdnfgdwt ddiavalgsd
       61 vlrrcigypa ervrlrlykt wlanfaaqew pdtfdrdlle sivatpspsd ptkplfgdlf
      121 rqaavrprpe gtyldtstls plfkrpavld adfktakgiv ayldgllsvg drealheagl
      181 iigflsllls svsavsvnrl igifrnlekf glatvlpttf sgefpcptsa nlenlrdkcg
      241 wgqsalrpyi palavaqytl fmraideapk edvlflgqsf mvhtkysgld cvdllemfak
      301 rtrfpmdyln lllggnasaw trklmefyls nvaspgntqn sapwcrvvdp tffrglevgd
      361 grsyalkmmc fltrsqrgll yrkwsrlane tmseseveda rvwadnfrtl lakgklrpve
      421 astasatn
//
LOCUS       XP_077485499             428 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096046 [Amblyomma americanum].
ACCESSION   XP_077485499
VERSION     XP_077485499.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629373.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..428
                     /product="uncharacterized protein LOC144096046"
                     /calculated_mol_wt=47925
     CDS             1..428
                     /gene="LOC144096046"
                     /coded_by="XM_077629373.1:83..1369"
                     /db_xref="GeneID:144096046"
ORIGIN      
        1 msmvmrkwdy ftknrhqffs tfvtsddaar ldvkdetyvp fitdnfgdwt ddiavalgsd
       61 vlrrcigypa ervrlrlykt wlanfaaqew pdtfdrdlle sivatpspsd ptkplfadlf
      121 rqaavrprpe gtyldtsals plfkrpavld adfktakgiv ayldgllsvg drealheagl
      181 iigflsllls svsavsvnrl igifrnlekf glaavlptsf sgefpcptsa nlenlrdkcg
      241 wgqsalrpyi palavaqytl fmraideapk edvlflgqsf mvhtkysgld cvdllemfak
      301 rtrfpmdyln lllggnasaw trklmefyls nvaspgntqk sapwcrvvdp tffrglevgd
      361 grsyalkmmc fltrsqrgll yrkwsrlane tmseseveda rvwadniral lakgklrpve
      421 asrasatn
//
LOCUS       XP_077485500             428 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096062 [Amblyomma americanum].
ACCESSION   XP_077485500
VERSION     XP_077485500.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629374.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..428
                     /product="uncharacterized protein LOC144096062"
                     /calculated_mol_wt=47936
     CDS             1..428
                     /gene="LOC144096062"
                     /coded_by="XM_077629374.1:85..1371"
                     /db_xref="GeneID:144096062"
ORIGIN      
        1 msmvmrkwdy fsknrhqffs tfvtsddaar ldvkdetyvp fitdnfgdwt ddiavalgsd
       61 vlrrcigypa ervrlrlykt wlanfaaqew pdtfdrdlle sivatpspsd ptkplfgdlf
      121 rqaavrprpe gtyldtsals plfkrpavld adfktakgiv ayldgllsvg drealheagl
      181 iigflsllls svsavsvnrl igifrnlekf glatvlpttf sgefpcptsa nlenlrdkcg
      241 wgqsalrpyi palavaqytl fmraideapk edvlflgqsf mvhtkysgld cvdllemfak
      301 rtrfpmdyln lllggnasaw trklmefyls nvaspgntqn sapwcrvvdp tffrglevgd
      361 grsyalkmmc fltrsqrgll yrkwsrlane tmseseveda rvwadnfrtl lakgklrpve
      421 astasatn
//
LOCUS       XP_077485501             428 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096078 [Amblyomma americanum].
ACCESSION   XP_077485501
VERSION     XP_077485501.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629375.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..428
                     /product="uncharacterized protein LOC144096078"
                     /calculated_mol_wt=47832
     CDS             1..428
                     /gene="LOC144096078"
                     /coded_by="XM_077629375.1:123..1409"
                     /db_xref="GeneID:144096078"
ORIGIN      
        1 msmvmrkwdy fsknrhqffs tfvtsddaar ldvkdetyvp fitdnfgdwt ddiavalgsd
       61 vlrrcigypa ervrlrlykt wlanfaaqew pdtfdrdlle sivatpspsd ptkplfgdlf
      121 rqaavrprpe gtyldtsals plfkrpavld adfktakglv ayldgllsvg drealheagl
      181 iigflsllls svsavsvnrl igifrdlekf glaavlptsf sgefpcptsa nlenlrdkcg
      241 wgqsalrpyi palavaqytl fmraideapk edvlflgqsf mvhtkysgld cvdlleifak
      301 rtrfpmdyln lllggnasaw trklmefyls nvaspgntqk sapwcrvvdp tffrglevgd
      361 grsyalkimc flarsqrgll yrkwsrlane tmseseveda rvwadniral lakgklrpve
      421 asrasatn
//
LOCUS       XP_077485502             428 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096094 [Amblyomma americanum].
ACCESSION   XP_077485502
VERSION     XP_077485502.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629376.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..428
                     /product="uncharacterized protein LOC144096094"
                     /calculated_mol_wt=47904
     CDS             1..428
                     /gene="LOC144096094"
                     /coded_by="XM_077629376.1:85..1371"
                     /db_xref="GeneID:144096094"
ORIGIN      
        1 msmvmrkwdy ftknrhqffs tfvtsddaar ldvkdetyvp fitdnfgdwt ddiavalgsd
       61 vlrrcigypa ervrlrlykt wlanfaaqew pdtfdrdlle sivatpspsd ptkplfadlf
      121 rqaavrprpe gtyldtsals plfkrpavld adlktakglv ayldgllsvg drealheagl
      181 iigflsllls svsavsvnrl igifrnlekf glatvlptsf sgefpcptsa nlenlrdkcg
      241 wgqsalrpyi palavaqytl fmraideapk edvlflgqsf mvhtkysgld cvdllemfak
      301 rtrfpmdyln lllggnasaw trklmefyls nvaspgntqn sapwcrvvdp tffrglevgd
      361 grsnalkimc fltrsqrgll yrkwsrlane tmseseveda rvwadnfrtl lakgklrpve
      421 asrasatn
//
LOCUS       XP_077485503             428 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096094 [Amblyomma americanum].
ACCESSION   XP_077485503
VERSION     XP_077485503.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629377.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..428
                     /product="uncharacterized protein LOC144096094"
                     /calculated_mol_wt=47904
     CDS             1..428
                     /gene="LOC144096094"
                     /coded_by="XM_077629377.1:56..1342"
                     /db_xref="GeneID:144096094"
ORIGIN      
        1 msmvmrkwdy ftknrhqffs tfvtsddaar ldvkdetyvp fitdnfgdwt ddiavalgsd
       61 vlrrcigypa ervrlrlykt wlanfaaqew pdtfdrdlle sivatpspsd ptkplfadlf
      121 rqaavrprpe gtyldtsals plfkrpavld adlktakglv ayldgllsvg drealheagl
      181 iigflsllls svsavsvnrl igifrnlekf glatvlptsf sgefpcptsa nlenlrdkcg
      241 wgqsalrpyi palavaqytl fmraideapk edvlflgqsf mvhtkysgld cvdllemfak
      301 rtrfpmdyln lllggnasaw trklmefyls nvaspgntqn sapwcrvvdp tffrglevgd
      361 grsnalkimc fltrsqrgll yrkwsrlane tmseseveda rvwadnfrtl lakgklrpve
      421 asrasatn
//
LOCUS       XP_077485504             167 aa            linear   INV 25-NOV-2025
DEFINITION  antimicrobial peptide microplusin-like [Amblyomma americanum].
ACCESSION   XP_077485504
VERSION     XP_077485504.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629378.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..167
                     /product="antimicrobial peptide microplusin-like"
                     /calculated_mol_wt=16921
     CDS             1..167
                     /gene="LOC144096146"
                     /coded_by="XM_077629378.1:84..587"
                     /db_xref="GeneID:144096146"
ORIGIN      
        1 mkfllacall actaalasar qaaicfased qikeraacvr dqagefnarl ddavaslgct
       61 ddlcaiqklc tfvstdralt alfarpettf lrnlslrcta aaafagapga afpgaalsga
      121 avpvaavgpa flapqpaffa pkgfgvrafg arafgvgafr paafgpw
//
LOCUS       XP_077485505             167 aa            linear   INV 25-NOV-2025
DEFINITION  antimicrobial peptide microplusin-like [Amblyomma americanum].
ACCESSION   XP_077485505
VERSION     XP_077485505.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629379.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..167
                     /product="antimicrobial peptide microplusin-like"
                     /calculated_mol_wt=16921
     CDS             1..167
                     /gene="LOC144096146"
                     /coded_by="XM_077629379.1:84..587"
                     /db_xref="GeneID:144096146"
ORIGIN      
        1 mkfllacall actaalasar qaaicfased qikeraacvr dqagefnarl ddavaslgct
       61 ddlcaiqklc tfvstdralt alfarpettf lrnlslrcta aaafagapga afpgaalsga
      121 avpvaavgpa flapqpaffa pkgfgvrafg arafgvgafr paafgpw
//
LOCUS       XP_077485506             111 aa            linear   INV 25-NOV-2025
DEFINITION  antimicrobial peptide microplusin-like [Amblyomma americanum].
ACCESSION   XP_077485506
VERSION     XP_077485506.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629380.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..111
                     /product="antimicrobial peptide microplusin-like"
                     /calculated_mol_wt=12633
     CDS             1..111
                     /gene="LOC144096174"
                     /coded_by="XM_077629380.1:139..474"
                     /db_xref="GeneID:144096174"
ORIGIN      
        1 mkklvlfffv icaygfvsas addpdicsyd diklgillrc vranawlmlr ngldhlmhvy
       61 qctsdvcsfn kicdegdvet alkkymkakp hrefwneakk cekelppdva h
//
LOCUS       XP_077485507              99 aa            linear   INV 25-NOV-2025
DEFINITION  antimicrobial peptide microplusin-like [Amblyomma americanum].
ACCESSION   XP_077485507
VERSION     XP_077485507.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629381.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..99
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..99
                     /product="antimicrobial peptide microplusin-like"
                     /calculated_mol_wt=11063
     CDS             1..99
                     /gene="LOC144096190"
                     /coded_by="XM_077629381.1:117..416"
                     /db_xref="GeneID:144096190"
ORIGIN      
        1 mkvlfvflal avltasaghl elcgfntqkl arvtlcielk askelrltla karygenclt
       61 diclfrklce qgelgetlkt yltdeeieel heiaeecer
//
LOCUS       XP_077485509             109 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096206 [Amblyomma americanum].
ACCESSION   XP_077485509
VERSION     XP_077485509.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629383.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..109
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..109
                     /product="uncharacterized protein LOC144096206"
                     /calculated_mol_wt=11828
     CDS             1..109
                     /gene="LOC144096206"
                     /coded_by="XM_077629383.1:56..385"
                     /db_xref="GeneID:144096206"
ORIGIN      
        1 mmktiaycll lvaligvara dpsegtstcq ldddslktlv scvtpkagge lskkldsvrq
       61 rlecpdiecg vrkiceknng slehtsneyf teeekasira lfkncrgqs
//
LOCUS       XP_077485510              84 aa            linear   INV 25-NOV-2025
DEFINITION  antimicrobial peptide microplusin-like [Amblyomma americanum].
ACCESSION   XP_077485510
VERSION     XP_077485510.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629384.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..84
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..84
                     /product="antimicrobial peptide microplusin-like"
                     /calculated_mol_wt=9148
     CDS             1..84
                     /gene="LOC144096222"
                     /coded_by="XM_077629384.1:205..459"
                     /db_xref="GeneID:144096222"
ORIGIN      
        1 medknsvqlk selqcirrsi staanrsfdn avrnlgcqds acvirnlwvg ndlekamakh
       61 ftrseiteah naatacdpsa ghhh
//
LOCUS       XP_077485511             109 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096270 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077485511
VERSION     XP_077485511.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629385.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..109
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..109
                     /product="uncharacterized protein LOC144096270 isoform X1"
                     /calculated_mol_wt=11535
     CDS             1..109
                     /gene="LOC144096270"
                     /coded_by="XM_077629385.1:285..614"
                     /db_xref="GeneID:144096270"
ORIGIN      
        1 mltiwssavs lygrdircvl rpmikttcss ranssefgaa srqlptevst trpgisaakt
       61 ahassescas ettckkpwqs ilrgvksrks ttqpllaipa lvtttesyg
//
LOCUS       XP_077485512              94 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096270 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077485512
VERSION     XP_077485512.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629386.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..94
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..94
                     /product="uncharacterized protein LOC144096270 isoform X2"
                     /calculated_mol_wt=9775
     CDS             1..94
                     /gene="LOC144096270"
                     /coded_by="XM_077629386.1:285..569"
                     /db_xref="GeneID:144096270"
ORIGIN      
        1 mltiwssaik ttcssranss efgaasrqlp tevsttrpgi saaktahass escasettck
       61 kpwqsilrgv ksrksttqpl laipalvttt esyg
//
LOCUS       XP_077485513              69 aa            linear   INV 25-NOV-2025
DEFINITION  antimicrobial peptide microplusin-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077485513
VERSION     XP_077485513.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629387.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..69
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..69
                     /product="antimicrobial peptide microplusin-like isoform
                     X3"
                     /calculated_mol_wt=7349
     CDS             1..69
                     /gene="LOC144096270"
                     /coded_by="XM_077629387.1:285..494"
                     /db_xref="GeneID:144096270"
ORIGIN      
        1 mltiwssaan rsfdnaarnl gcqdsacvir klcvgndlqk amakhftrse itevhnaata
       61 cdpsaghhh
//
LOCUS       XP_077485514             146 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096306 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077485514
VERSION     XP_077485514.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629388.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..146
                     /product="uncharacterized protein LOC144096306 isoform X1"
                     /calculated_mol_wt=15870
     CDS             1..146
                     /gene="LOC144096306"
                     /coded_by="XM_077629388.1:67..507"
                     /db_xref="GeneID:144096306"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrppfa altsvlccqr pasigpcvlp afgcvippdp
       61 fmlktkchme vvqatklrvi fardleqagk lvdgqwysls kasffsktkt iflgswamyl
      121 gtcgqdnagq hasasskkkh etvnky
//
LOCUS       XP_077485515             140 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096306 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077485515
VERSION     XP_077485515.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629389.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..140
                     /product="uncharacterized protein LOC144096306 isoform X2"
                     /calculated_mol_wt=15216
     CDS             1..140
                     /gene="LOC144096306"
                     /coded_by="XM_077629389.1:67..489"
                     /db_xref="GeneID:144096306"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrppfa altsvlccqr pasigpcvlp afgcvippdp
       61 fmlktkchme vvqatklrvi fardleqagk lvdgqwyslt ktktiflgsw amylgtcgqd
      121 nagqhasass kkkhetvnky
//
LOCUS       XP_077485516             381 aa            linear   INV 25-NOV-2025
DEFINITION  G-protein coupled receptor dmsr-1-like [Amblyomma americanum].
ACCESSION   XP_077485516
VERSION     XP_077485516.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629390.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..381
                     /product="G-protein coupled receptor dmsr-1-like"
                     /calculated_mol_wt=42371
     Region          52..352
                     /region_name="7tmA_FMRFamide_R-like"
                     /note="FMRFamide (Phe-Met-Arg-Phe) receptors and related
                     proteins, member of the class A family of
                     seven-transmembrane G protein-coupled receptors; cd14978"
                     /db_xref="CDD:410630"
     Region          52..78
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410630"
     Region          84..109
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410630"
     Site            order(106,109..110,126..131,133..134,137,182,184..188,221,
                     224..226,228..230,232..233,296,299..300,302..303,306,
                     319..320,322..324,327,330..331)
                     /site_type="other"
                     /note="putative ligand binding pocket [chemical binding]"
                     /db_xref="CDD:410630"
     Region          126..156
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410630"
     Region          168..188
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410630"
     Region          221..246
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410630"
     Region          277..307
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410630"
     Region          320..345
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410630"
     CDS             1..381
                     /gene="LOC144096344"
                     /coded_by="XM_077629390.1:22..1167"
                     /db_xref="GeneID:144096344"
ORIGIN      
        1 mlqivdslyl deenlcrtea nlcgphgive keelpyhgae lgrfcawyts hlhgylsmpv
       61 cvfgilanvl siivltqrnm vsptngiltg lavadmlvia tylpytitth vmpqeckqsf
      121 eravfvlvhs hvsvvfhtvs twltvtlavw rflavsfpas skewcsmqra rwavvsvyvs
      181 calcclplyl sltvhqigtp qepdyivnft nitlandafl rnldfwtysv lmklvpcval
      241 tglslgllrv lykakarerr lrkgdcghtg hggnsegdre rttrmllavl llflvtelps
      301 gilallsgil gqdflnhvyn klgavmdila lvnsavnfil ycsmsrqfrk afaalfmpri
      361 vpkwfpltsk ppnstyattc v
//
LOCUS       XP_077485518             829 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077485518
VERSION     XP_077485518.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629392.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..829
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=92003
     Region          111..244
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          340..750
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..829
                     /gene="LOC144096403"
                     /coded_by="XM_077629392.1:216..2705"
                     /db_xref="GeneID:144096403"
ORIGIN      
        1 migrrteprr sravgvaciv faiaycccwc cadvalaegn vapalgddek asavmddlts
       61 ssedadsaap ppkmtwegfm hnmttgfatv lhkifplmvr asseveigpe cmasyfklfl
      121 glrklkgwav rlvdasgkpa dgllegtlaf vgafdecldt vvwdeqdssr llfrgrycta
      181 qvapkftlrd lfhndsqayt elasflpkta mlknalripg nhvifrvglc vpstcssddi
      241 ermvkytvkh mdmkaevtec iqreenkpls aiqitvitll aaflsltvig tvtdvtlker
      301 rhpkaaassk hgrpleallc fsaysnarkl lapedkpdsl ralhgirfls mtwiifghsy
      361 ffiehvqpfr gifnghemys enfffsgvin ftlavdsfff isgllvvytn wkeltesngr
      421 lnvirflfnk ywrmvpplll slgllflmpv lgdgpfwndi mgteirlcek swwsnlllin
      481 nfweskqmcl vatwylacnf qffvlsvfil iplynwpvvg lgatfmllla gsvvsgvitf
      541 laelppglif ypdldtvsnl vtyvyhkpyn higsycvgvf lgyiivrhrd iklkpltqvv
      601 gwcasfsvgv avlwaayrwn aelpgpsvaa lyaathrvaw cvalawltfa cvaghggfld
      661 sllswppfna lgnlaymayl mhplvilyhy srtrdllyys qyekvyafcg hflitlllst
      721 ffyvvvempf arlggmllrt rlfrkpsrra agvsagsaes garrkpafai vagitrgvap
      781 lafikaraga hgtakrsssa qtaaelstsg dggrptngrf rktgdsshl
//
LOCUS       XP_077485519             804 aa            linear   INV 25-NOV-2025
DEFINITION  O-acyltransferase like protein-like [Amblyomma americanum].
ACCESSION   XP_077485519
VERSION     XP_077485519.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629393.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..804
                     /product="O-acyltransferase like protein-like"
                     /calculated_mol_wt=89351
     Region          77..208
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          305..677
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..804
                     /gene="LOC144096419"
                     /coded_by="XM_077629393.1:1..2415"
                     /db_xref="GeneID:144096419"
ORIGIN      
        1 merravvcaf lvflcslvav crggqaqgpf qglqdamtnt swedqvnksk vnidavanei
       61 fpyvvegsse ldvsvdcmta llktfkafrd lkgwavrlmd asgklpegll egrmasigsy
      121 decldlvaey ktqelyrgky ctvylssghn vqemfnnesd asahlstllr hpkrmtrivy
      181 rvspksrkfr lgvcvpsvct lddvqnvvah tssksigakt hvsrcevked vsitglqvfl
      241 iitlcllvgv ilvatildmr veanaqkalp gagcltncll sfsfarnlrr ltdpveppel
      301 gaaqgvqais mawlicghty fltenthmfs gllraydsis ndilfspfvn ytlaantffl
      361 tsglflaykc elysyksvfs illqkyfrmt ppflavlsvl lllpltnngp fwedyagaei
      421 ercsaawwtn ifyisnlwsa drmclfvtwy patvfqytvi gliflivikr nakigycsvl
      481 gfalagcvvt gaitfakdlp pgsifepnyr everlstwiy hkpythlgsf cvglglgcll
      541 sqfdknrlsk layamgwvas ltttsvlfhc gfgwsldras aagaalyaal hrtlwalavs
      601 wvlyscctlh arllndwlsw gplkvvgrls ycmllvhplv ivsqksqnrh itffshfdmm
      661 hlffghyavt lvlsffvhlg veepfaqlge llgscevscp prlkqltqnl yagmatqlrr
      721 reqqqqqqpa scrkeisdgk kpqnqqqppq ednhhteiti dadgggrqle ngtevavike
      781 ngrdmshgvr dghrnsaanp easv
//
LOCUS       XP_077485520             174 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096462 [Amblyomma americanum].
ACCESSION   XP_077485520
VERSION     XP_077485520.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629394.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..174
                     /product="uncharacterized protein LOC144096462"
                     /calculated_mol_wt=19194
     CDS             1..174
                     /gene="LOC144096462"
                     /coded_by="XM_077629394.1:65..589"
                     /db_xref="GeneID:144096462"
ORIGIN      
        1 mhidelftsl kamppqqpps tianasiayi agfvaravee rrtcsfcppl qqsdgpspvl
       61 mglidlqsrg glifpksefv tvlvtvkkav didlphikks nvrqqlaelf lprleqcplf
      121 vcparddhaa stlyvvfdkf mrpllanvga tvadraayrk kltckplhrk vlrv
//
LOCUS       XP_077485521             395 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096479 [Amblyomma americanum].
ACCESSION   XP_077485521
VERSION     XP_077485521.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629395.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..395
                     /product="uncharacterized protein LOC144096479"
                     /calculated_mol_wt=41361
     Region          30..113
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     CDS             1..395
                     /gene="LOC144096479"
                     /coded_by="XM_077629395.1:28..1215"
                     /db_xref="GeneID:144096479"
ORIGIN      
        1 mgsqqfclkw nnhqsnmlav fyqmlsneal vdvtlacegl slkahkmvls acspffqavf
       61 aenpckhpiv ilkdvryvdl raivefmyrg evvvsqedlm allktaetlk vrglvtsvdg
      121 aadsgpvgsp raeapplskr krsrrprrrs psdsnksdse dappatrikv pdspeiiddg
      181 smssdhmtdp htaspasras nmasstavps garhgssvqa sisqhtqdsi gddvgegdda
      241 dfdvepldvm eesattesvp vfpdvasssq algdesqqsh htgstsgnsa lvpaqpslps
      301 dipitsqvdi epsptsltpy ddktiapvaa aspaaassgd gastsmaatd asgvpaiagp
      361 sgyqqstpsh gtypvpdhps hlhlsfcvtf aprls
//
LOCUS       XP_077485522             420 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096495 [Amblyomma americanum].
ACCESSION   XP_077485522
VERSION     XP_077485522.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629396.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..420
                     /product="uncharacterized protein LOC144096495"
                     /calculated_mol_wt=44127
     Region          30..113
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     CDS             1..420
                     /gene="LOC144096495"
                     /coded_by="XM_077629396.1:15..1277"
                     /db_xref="GeneID:144096495"
ORIGIN      
        1 mgsqqfclkw nnhqsnmlav fyqmlsneal vdvtlacegl slkahkmvls acspffqavf
       61 aenpckhpiv ilkdvryvdl raivefmyrg evvvsqedlm allktaetlk vrglvtsvdg
      121 aadsgpvgsp raeapplskr krsrrprrrs psdsnksdse dappatrikv pdspeiiddg
      181 smssdhmtdp htaspasras nmasstavps garhgssvqa sisqhtqdsi gddvgegdda
      241 dfdvepldvm eesattesve svpvtiakha lssydtpffi ssqvpvfpdv asssqalgde
      301 sqqshhtgft sgnsalvpaq pslpsdipit sqvdiepspt sltpyddkti apvaaaspaa
      361 assgdgasts maatdasgvp aiagpsgyqq stpshgtypv pdhpshlhls fcvtfaprls
//
LOCUS       XP_077485524             237 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096527 [Amblyomma americanum].
ACCESSION   XP_077485524
VERSION     XP_077485524.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629398.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..237
                     /product="uncharacterized protein LOC144096527"
                     /calculated_mol_wt=26766
     CDS             1..237
                     /gene="LOC144096527"
                     /coded_by="XM_077629398.1:1193..1906"
                     /db_xref="GeneID:144096527"
ORIGIN      
        1 mmqkwfailn vanrtahvhm rkpdqmhffc asderlqwle kefpdyletl ynackrsgkk
       61 flsaetykai lltskstvlc ikyllesgff yvltrnspsd pvellfsslr kmaggnhcld
      121 aravtfsler ilrtdslyps qvsnvengqt vlsmhgasln vtsseldvpa eataqatthd
      181 stetaqvapa rftrteyfvm hidelftslk ampreclhpr evppksdhfa lcqgynn
//
LOCUS       XP_077485525            1749 aa            linear   INV 25-NOV-2025
DEFINITION  intraflagellar transport protein Oseg2 [Amblyomma americanum].
ACCESSION   XP_077485525
VERSION     XP_077485525.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629399.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1749
                     /product="intraflagellar transport protein Oseg2"
                     /calculated_mol_wt=197503
     Region          <7..319
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          19..64
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          70..107
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          115..149
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          157..195
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          200..234
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          244..283
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          692..968
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Region          862..1083
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Region          1211..1441
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     CDS             1..1749
                     /gene="Oseg2"
                     /coded_by="XM_077629399.1:73..5322"
                     /db_xref="GeneID:144096543"
ORIGIN      
        1 mqikylktlm hpqdgaakvv amawspnnvk laactadrvv llfdemgerr dkfstkpids
       61 kygkksyvvk aicfspdstk lavaqtdnii yvykigedwg ekkvicnkfi qqsavtcmaw
      121 phhgpiafgl adgkvrsanv ktnksstlyg tesyvvsvvs sisgkgilsg hadgsvvryf
      181 ltdddsgevq gkvlthpcpp yalcwteqsi maagcdkrii fyapregrvq qqfdfsrqpe
      241 erefavagcs pgghalavgs ydrvrvftws sskrawdeak pkelhnayti sslawkrdgs
      301 rlavgtlcga ilsydcslrk tlynnkfeit fvgpsqaliv nlatktkstl ksqygheiqd
      361 ikilgrdryl vaytaatlll gdllqdkfce vfwegtsrne kfyfetenvc mifnagelsl
      421 veyghneilg svrtefmnph llsvrlnerr ragapdnkkl aylidlktif vvdlvtgmtl
      481 aqvphdskid wlelnetgqk llfrdrrqrl slfdiatqak vtllnnctfv qwvpdsdvvv
      541 aqsrsvlyvw ynvdapertt nipikgdvvd ieredgktev ivneglttvg yplkeglief
      601 gtaitdgdfg ravaylesle lteetetmwr tlgqmaleer lvavaersya algdlararf
      661 lahvirlqed aaratgqdgn dhyevrarma mlekqfklae niyleqnkve eamemyknmh
      721 kwdemiqlae srnyhnvdkl redyfewlls ngmegraael keregdaeea vslylksglp
      781 akaalvlrsv pglvqqrhla drviaaltqs elyehagglh ellgehakal qcyraghhya
      841 kavdlsrqrn pgdvvaleee wgeylvsqrq ldaainhfie agktvkalda ainarqwkka
      901 vqiieviddt pelsqyqskl aqhfhatgdl emaehfyvea gkikeaiemy nsagkwekal
      961 klaklhlrpe dvtsmyteqa selekqgklk eaeklylfie epdlaiamyk kwrqydnmmr
     1021 vvkefhpdll sdthmhlake legegnylqa eahyiaasdw kaavnmyran dmweeayava
     1081 kkhggpvack qvaylwaksl ggdsavrlls klglldstid yaaencafdf afelaqatak
     1141 hklpdihlky amfledegkf heaelefika nkpkeavlmy vhnkdwdsar rvaeahdpes
     1201 vadvsigqar vafqaghyqq aeslalraer pdlvikmykd agmwndalri creytpsklm
     1261 evqeeydnhm dtkgardvda lleqaqewer ngqydravdc ymrmtpenvt nvdmlekcwv
     1321 kaadlalkfl deqkadrilk naarmlldik khssaaqlfl smelvkeavd aliaggewsk
     1381 akkvaqefep rleehvdkey knflrsrgqt dqlanvdiia aldlyaekqq wkkclemaeq
     1441 qgpqvlhkyv alcasqrird gspldalrly adhgapalsq nlniyrhiaa svfalpglyd
     1501 aqayrtwsel rdvlhdlvkn leasgqdvgd fdklllisny yatrsacqgq etlaehamki
     1561 svsllrytey ipadkafyea glacktmnre gmafvfwnhy ldiceaieeg sldtldhsdf
     1621 qntdipfevp lpekmfldea qhedvkewvl avsmnqgveq alptdeqgfy easlqgpkgt
     1681 lralpclitg ypvlqnklqf qkpgiacnkd dwnkfllatk lsrsaecqdv lkfitewagg
     1741 tpnlsysfq
//
LOCUS       XP_077485526             284 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096559 [Amblyomma americanum].
ACCESSION   XP_077485526
VERSION     XP_077485526.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629400.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..284
                     /product="uncharacterized protein LOC144096559"
                     /calculated_mol_wt=31252
     CDS             1..284
                     /gene="LOC144096559"
                     /coded_by="XM_077629400.1:31..885"
                     /db_xref="GeneID:144096559"
ORIGIN      
        1 mapvgkltlr lllvvlvagl gvitaraqeq aaednqqaqp aarsvivaes agvpllgspa
       61 lapashqeyv aaqpaatyda vprsrlrrta lslrrrrrld npryyypngd ysadteiswd
      121 nyhrraarey aeaarrpdsy qasyaaplep eaerfkadeg hreadygaag ydyrsgyqrq
      181 gyqkqrraam pplrqqpeqw glwqqdygtq gqqslkvryp qydygeaaaa yarkdpssfy
      241 areeaasrae vgnnippsny aaevvgqdgv veragdvpva aaak
//
LOCUS       XP_077485527             198 aa            linear   INV 25-NOV-2025
DEFINITION  heme-binding protein 2-like [Amblyomma americanum].
ACCESSION   XP_077485527
VERSION     XP_077485527.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629401.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..198
                     /product="heme-binding protein 2-like"
                     /calculated_mol_wt=23212
     Region          26..195
                     /region_name="SOUL"
                     /note="SOUL heme-binding protein; pfam04832"
                     /db_xref="CDD:461449"
     CDS             1..198
                     /gene="LOC144096575"
                     /coded_by="XM_077629401.1:1..597"
                     /db_xref="GeneID:144096575"
ORIGIN      
        1 mrtfrviiaa viiqvvaays ctwrgvecid yevisneaey eerqypetmw isvttqaral
       61 feakrksfrk liyyiqgfnd qnvtvdlttp hrtlvsrgas grrlanytma fplpanlymn
      121 ppqandkdiv itvepprkya vkvfggqpne qqwlskaeef iqsmrkdrri nldhfyvary
      181 dlifwvlerr neiwlsmk
//
LOCUS       XP_077485548             276 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096609 [Amblyomma americanum].
ACCESSION   XP_077485548
VERSION     XP_077485548.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629422.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..276
                     /product="uncharacterized protein LOC144096609"
                     /calculated_mol_wt=29856
     Region          16..144
                     /region_name="DUF4779"
                     /note="Domain of unknown function (DUF4779); pfam16009"
                     /db_xref="CDD:464971"
     CDS             1..276
                     /gene="LOC144096609"
                     /coded_by="XM_077629422.1:293..1123"
                     /db_xref="GeneID:144096609"
ORIGIN      
        1 matgygghhs gygghknygg hhshgryggg ykhhgndydn hhgnhgrygg enhghgydry
       61 hsnhdgkhgq gsyggkhygn dqhgghgkhg gkygndhyka rghknyghkn vyhkedsahh
      121 sryyddyhdg dhkkkynqhh nyhdhhagrr hhghhhkqhh dkhrhgddyh khghghyksh
      181 hgqhgghggn hyrggsghky ggggyhrgsh dhyggqkhhg ggghggggyg ghggggyggh
      241 ggggyggghg ggyasngvpt yikvpasqts faatfr
//
LOCUS       XP_077485558             363 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096615 [Amblyomma americanum].
ACCESSION   XP_077485558
VERSION     XP_077485558.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629432.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..363
                     /product="uncharacterized protein LOC144096615"
                     /calculated_mol_wt=40424
     CDS             1..363
                     /gene="LOC144096615"
                     /coded_by="XM_077629432.1:21..1112"
                     /db_xref="GeneID:144096615"
ORIGIN      
        1 mgikypndet rrslyaswlr vlasnqwhns fdglmlecla atpspttpeq plfaniysvr
       61 hpadgpipdi lapvlnlgql pdlftherep veplptsagf vdyladlick ggtsraalde
      121 agliigfcsl alavatskpp eevraffrhq lkrelvaavp tsfngdmfip eerflcnlsf
      181 ksltdsgvvk pffvllvlgq yayhlnaeaa piadirflee gflnktkygr lemvqlqyav
      241 qeqsgldake lnrlirshga lrsviesssv vaafldhaek pmqktmpwcl vanrsvcldl
      301 dlsehldyai rltallapdp nhkfwrrpef agvslshiqe sllwasvfrr ymqnkknens
      361 aap
//
LOCUS       XP_077485581             279 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096629 [Amblyomma americanum].
ACCESSION   XP_077485581
VERSION     XP_077485581.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629455.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..279
                     /product="uncharacterized protein LOC144096629"
                     /calculated_mol_wt=32080
     Region          50..126
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..279
                     /gene="LOC144096629"
                     /coded_by="XM_077629455.1:130..969"
                     /db_xref="GeneID:144096629"
ORIGIN      
        1 msapwdhsgy tspaevvltt mideeedhtm pevmdsskkp netavsiasw derqtkllld
       61 yyaryardvg plkkfrskra mfeqiacdmf gilgihrtav qcetrfktvs krkrneekhn
      121 ntsgnsrcrv eyeqefdaik aiddslepev lrgvdtvryk kastckaspr pstsadvqen
      181 sefsgdtlsl issggdcsfe agtdsqdeep kkkradrrlr psnsrmqhlq lffdemnklh
      241 rekekkkedr eqrkeqrhqe llkshsehma llktlaeke
//
LOCUS       XP_077485622             224 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096660 [Amblyomma americanum].
ACCESSION   XP_077485622
VERSION     XP_077485622.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629496.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..224
                     /product="uncharacterized protein LOC144096660"
                     /calculated_mol_wt=24930
     CDS             1..224
                     /gene="LOC144096660"
                     /coded_by="XM_077629496.1:249..923"
                     /db_xref="GeneID:144096660"
ORIGIN      
        1 mselgprqdn vlrpgtnvdi rrtdgrihpa vvsavrphlv tvewfengqt kgkevdrqtl
       61 valnpglasp rrndstgslg aptphrktta gslpctpare eedperaaql glalsrkkte
      121 agarqqlrnq lveqeeaeav adllrryrav vdvsdlwmtt dvrlltrmls traleddtwl
      181 releelltvk mellsnlrda vnnlrsvkds trtfirdaeg ngkh
//
LOCUS       XP_077485634             128 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096668 [Amblyomma americanum].
ACCESSION   XP_077485634
VERSION     XP_077485634.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629508.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..128
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..128
                     /product="uncharacterized protein LOC144096668"
                     /calculated_mol_wt=14312
     CDS             1..128
                     /gene="LOC144096668"
                     /coded_by="XM_077629508.1:182..568"
                     /db_xref="GeneID:144096668"
ORIGIN      
        1 magggrrqrp vpsannermk rpgegkkgsg aeershacrr erkrrrqrrw aasrrrkgsp
       61 pprlarwqav ivqtqflcvr yqelcldira chwshrcgvc rspatlarcg pasgtsrgrt
      121 ptgrlagk
//
LOCUS       XP_077485642             229 aa            linear   INV 25-NOV-2025
DEFINITION  RPA-interacting protein B-like [Amblyomma americanum].
ACCESSION   XP_077485642
VERSION     XP_077485642.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629516.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..229
                     /product="RPA-interacting protein B-like"
                     /calculated_mol_wt=25522
     Region          11..49
                     /region_name="RPA_interact_N"
                     /note="Replication protein A interacting N-terminal;
                     pfam14766"
                     /db_xref="CDD:464304"
     Region          63..142
                     /region_name="RPA_interact_M"
                     /note="Replication protein A interacting middle;
                     pfam14767"
                     /db_xref="CDD:434193"
     Region          152..227
                     /region_name="RPA_interact_C"
                     /note="Replication protein A interacting C-terminal;
                     pfam14768"
                     /db_xref="CDD:464305"
     CDS             1..229
                     /gene="LOC144096677"
                     /coded_by="XM_077629516.1:48..737"
                     /db_xref="GeneID:144096677"
ORIGIN      
        1 masscdsgls rhqllykskt ppwketyrnr crerlrkgrq alfesfrssg gachseqelr
       61 ysvtkvvree ldnfccssat sslglvassa enavpaapwd earlneelme etlaalleee
      121 fnvwkqyedi malqnqeiaa avhmwsselv acpvclkndl ekdgsriica cglaiptals
      181 lkefrlsier vveahclqcr sflsftfiet cepalvcvcn acdfmssvl
//
LOCUS       XP_077485657             285 aa            linear   INV 25-NOV-2025
DEFINITION  palmitoyltransferase ZDHHC2-like [Amblyomma americanum].
ACCESSION   XP_077485657
VERSION     XP_077485657.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629531.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..285
                     /product="palmitoyltransferase ZDHHC2-like"
                     /calculated_mol_wt=31187
     Region          <1..94
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; cl19890"
                     /db_xref="CDD:418707"
     Region          <120..267
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..285
                     /gene="LOC144096688"
                     /coded_by="XM_077629531.1:1190..2047"
                     /db_xref="GeneID:144096688"
ORIGIN      
        1 mnhhcpwfnn cvcfstykff lltifyvval cvfrlasath lvagawssna ctyvtlnctf
       61 lyafgvmlvl vlgsflylhf tmvcnnvttl edlrphlfkd drdsfdigfr dniaevfghr
      121 kalrflpvft slgdgarfpt rlhpdryvyg ppvtvvveer rlsvpvppla vtdevpalpv
      181 ltscrrpvps tegplggltl tpfvapprgs lsflppttit vvppteaaas vrlseskkdl
      241 ppivvasaft smevrllcat lpspqasreh sfycgmgcpg vrgrg
//
LOCUS       XP_077485667             841 aa            linear   INV 25-NOV-2025
DEFINITION  piwi-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077485667
VERSION     XP_077485667.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629541.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..841
                     /product="piwi-like protein 1"
                     /calculated_mol_wt=94940
     Region          323..455
                     /region_name="PAZ"
                     /note="This domain is named PAZ after the proteins Piwi
                     Argonaut and Zwille; smart00949"
                     /db_xref="CDD:198017"
     Site            order(364,377,384,388,406,421,423)
                     /site_type="other"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]"
                     /db_xref="CDD:239211"
     Region          442..824
                     /region_name="Piwi_piwi-like_Euk"
                     /note="PIWI domain, Piwi-like subfamily found in
                     eukaryotes. This domain is found in Piwi and closely
                     related proteins, where it is believed to perform a
                     crucial role in germline cells, via RNA silencing. RNA
                     silencing refers to a group of related...; cd04658"
                     /db_xref="CDD:240016"
     Site            order(611,615,627..630,633,643,646,650,654)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240016"
     CDS             1..841
                     /gene="LOC144096696"
                     /coded_by="XM_077629541.1:1..2526"
                     /db_xref="GeneID:144096696"
ORIGIN      
        1 meknncdrap ppvgrargrs rgraravvvl pgaqaaptvp qfpaamapla alprergvlr
       61 patvslpsmp aldsvaglss svhrmaighg aplqqmaaia psavvpqqfh fstavtpgds
      121 gdggrpeirr rrdefaviet rpahidnnrg tsgspikvla nyfrlismpk csihqyhvqf
      181 tptvessrmr rtllvdymdm fekclvfdgm selmsptrlp qditevfpqr rtdgmticvr
      241 fkwvqelapt npdvlrlfnt qmrrnlehld fvqinrhffd kravwivpqy gfelwqglvt
      301 aigqhesgvm lrtnplhkvl rrdsvydlms qiehvpnyke ecvkrvagci vvtpyngkty
      361 rvddidwdkn pactfetmeg pktyadyyrv qyekrirdmr qpllvcrpkd kdlragrtqn
      421 iylvpelcvl telteemran vsmmrdlaqh tdvepskwvr nllefmarin nneairndmd
      481 swdllyddsl vridarvlpp ekvmqgsnay rysaatadfs retrdrplhv avaveswlvi
      541 chrreetnvt efvrtlmsvc ppmgvkigqp rlvlldddgp tdfvqalhql arggniqlal
      601 ivlhnnrkdr ydiimkeacv dlglhtqvvl artignstni hsvatnvavq lncklggeaw
      661 cleiplvstm vigydtcpds sthnrsagaf vvkrweidev vaslqalfpg vepklafvvv
      721 tkristrffl pdrehvtnpp pgtvvdsevt rpgrydfflv sqsvrqgava ptyynviydt
      781 tglkpddvqr lvyklthlyf nwpgtirvpa pcqyarklaf lagqslhaep hprlsstlfy
      841 l
//
LOCUS       XP_077485677             148 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096704 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077485677
VERSION     XP_077485677.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629551.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="uncharacterized protein LOC144096704 isoform X1"
                     /calculated_mol_wt=16759
     CDS             1..148
                     /gene="LOC144096704"
                     /coded_by="XM_077629551.1:262..708"
                     /db_xref="GeneID:144096704"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedrrr rhlnrrltat mkrlsgvhvl
       61 nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar pgevyvvhrc
      121 rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077485682              98 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096704 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077485682
VERSION     XP_077485682.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629556.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..98
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..98
                     /product="uncharacterized protein LOC144096704 isoform X2"
                     /calculated_mol_wt=10604
     CDS             1..98
                     /gene="LOC144096704"
                     /coded_by="XM_077629556.1:341..637"
                     /db_xref="GeneID:144096704"
ORIGIN      
        1 mkrlsgvhvl nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar
       61 pgevyvvhrc rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077485715             357 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096739 [Amblyomma americanum].
ACCESSION   XP_077485715
VERSION     XP_077485715.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629589.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..357
                     /product="uncharacterized protein LOC144096739"
                     /calculated_mol_wt=39183
     Region          147..170
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          167..>322
                     /region_name="AMN1"
                     /note="Antagonist of mitotic exit network protein 1;
                     cd09293"
                     /db_xref="CDD:187754"
     Region          201..227
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          228..253
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          254..279
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     CDS             1..357
                     /gene="LOC144096739"
                     /coded_by="XM_077629589.1:122..1195"
                     /db_xref="GeneID:144096739"
ORIGIN      
        1 mfsteiaqfl ktlltthvkn eqesfegpps vrqavlhrrg tkgyysfswq tatgekcisg
       61 tgkalprris kllkrhhagg glpclskism snatigaewk vvlssdvrkl tslcprlrev
      121 sfdgfsfgaa hrkrrvklrd fpqgmvslsl rgcvldmktf fsagptehge lttldlgrcf
      181 flnddkacsg krpswpslpa lerlclegcp flisdallsd vllccpalqv ldlegtllqr
      241 svdlamigkc lpnlrelfvg wtdvndstfl alqrgsfesl stlclagtav tnlgvlavcg
      301 vcpllrvvrv nmgrcavrrl envdactsvs fevacfrsdn vhavlrhegc ehfrqrv
//
LOCUS       XP_077485725             114 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096745 [Amblyomma americanum].
ACCESSION   XP_077485725
VERSION     XP_077485725.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629599.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..114
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..114
                     /product="uncharacterized protein LOC144096745"
                     /calculated_mol_wt=12767
     CDS             1..114
                     /gene="LOC144096745"
                     /coded_by="XM_077629599.1:1..345"
                     /db_xref="GeneID:144096745"
ORIGIN      
        1 msargaliva lalvaaahag pcgndedpdr aefwscfark lssteknvmt dlkntffrrs
       61 qkqlnyqwlc dqlsmparrr qqmaaylvqk frsaqraakv nehlascgrt vyqv
//
LOCUS       XP_077485733             768 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077485733
VERSION     XP_077485733.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629607.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..768
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=86875
     Region          87..219
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          307..718
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..768
                     /gene="LOC144096749"
                     /coded_by="XM_077629607.1:269..2575"
                     /db_xref="GeneID:144096749"
ORIGIN      
        1 mgllpswtll vlllvahaav aqttpeepat aptteepptt tpdaaeavrr swakvesmlk
       61 stvdtqlrrl lpqmlrasge agmspecqaa nfkvmlglrq lkswairlid stgrpaagtl
      121 egtlsdfgny declkirmvd ehtgeenfrg kyctlkfaap mppkperlny hqdifnttle
      181 egglledmar gapyfyhlap klgicipsac sgqdieslln vvlkpmdwna taincdvdgp
      241 itikknqlai iiviaiviil cvlgtilttf rrmcsqdpkn lrpnlffdim kcfsfydnaq
      301 klfntkssqg mlgaihgikf lsmcwvvlch syifmnperv valenihqdp ftwsfqimmn
      361 gwlavdtffm lggvtlayst lrhmaenkgr lnifiyffhr ilrivpplgf tlaliillpv
      421 fgrgpvwhei vdvqankceq nwwqtllffa nfvttydsic lettwylqcd mqyyilalai
      481 vlpmykhpvl gslinlmfli vsfiitavii vyynfpaimi flhpdidlik keafyihwrp
      541 ythygpyavg lflgllffkk qkirfskala ilgwaaaitl qlamiygird whehyetvgi
      601 vgyvfavthr tawaatvayv lfaaqfglgg wigqflkwka liplsrltfm tyllhplvmw
      661 ykqgnlrerf ylrhfpeman nvvsdlvfsy aaafiatllf evpmmnleki ilrpfagppk
      721 kqgrsgngls nsgyasespp vdfvinghhy pddykrsagg ernsssrl
//
LOCUS       XP_077485748             353 aa            linear   INV 25-NOV-2025
DEFINITION  protein Wnt-8a-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077485748
VERSION     XP_077485748.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629622.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..353
                     /product="protein Wnt-8a-like isoform X1"
                     /calculated_mol_wt=39569
     Region          72..352
                     /region_name="Wnt_Wnt8"
                     /note="Wnt domain found in protein Wnt-8a, Wnt-8b and
                     similar proteins; cd19340"
                     /db_xref="CDD:381714"
     Site            order(211..212,215..217,225,332,334,336,338)
                     /site_type="other"
                     /note="Frizzled receptor binding site [polypeptide
                     binding]"
                     /db_xref="CDD:381714"
     CDS             1..353
                     /gene="LOC144096751"
                     /coded_by="XM_077629622.1:19..1080"
                     /db_xref="GeneID:144096751"
ORIGIN      
        1 meakssrsrg awrwhsfhfa sihgrvgrgg rttgttlymk llstlfsggq aacclpviiv
       61 crevgraklg tidigvrqai decqaqfrwd rwncperafd ilrkrakppa tremafvqai
      121 msagitytia kncsrgnfrr ctcdhrprkv pkgvdwtwgg csdnvqfgyr vsrrflldrt
      181 kdsrdlyaii nkhnnragsf avrksmrrtc kchgpsgsca lktcwmqlge maavgdrlrr
      241 ayerarllis nrgepagtes rnvllytsps snfcqpnltl gyngtygrec srrtgvsvpl
      301 teqdscsklc ydcghglrqe aatesstcnc rfvwccsvkc dicttnvqkh fcn
//
LOCUS       XP_077485753             339 aa            linear   INV 25-NOV-2025
DEFINITION  protein Wnt-8a-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077485753
VERSION     XP_077485753.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629627.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..339
                     /product="protein Wnt-8a-like isoform X2"
                     /calculated_mol_wt=37900
     Region          58..338
                     /region_name="Wnt_Wnt8"
                     /note="Wnt domain found in protein Wnt-8a, Wnt-8b and
                     similar proteins; cd19340"
                     /db_xref="CDD:381714"
     Site            order(197..198,201..203,211,318,320,322,324)
                     /site_type="other"
                     /note="Frizzled receptor binding site [polypeptide
                     binding]"
                     /db_xref="CDD:381714"
     CDS             1..339
                     /gene="LOC144096751"
                     /coded_by="XM_077629627.1:376..1395"
                     /db_xref="GeneID:144096751"
ORIGIN      
        1 mvviacvlfk ltlfvlstta wssrndmlsg srtsgdavkl psakskqllv graklgtidi
       61 gvrqaidecq aqfrwdrwnc perafdilrk rakppatrem afvqaimsag itytiakncs
      121 rgnfrrctcd hrprkvpkgv dwtwggcsdn vqfgyrvsrr flldrtkdsr dlyaiinkhn
      181 nragsfavrk smrrtckchg psgscalktc wmqlgemaav gdrlrrayer arllisnrge
      241 pagtesrnvl lytspssnfc qpnltlgyng tygrecsrrt gvsvplteqd scsklcydcg
      301 hglrqeaate sstcncrfvw ccsvkcdict tnvqkhfcn
//
LOCUS       XP_077485760             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096759 [Amblyomma americanum].
ACCESSION   XP_077485760
VERSION     XP_077485760.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629634.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144096759"
                     /calculated_mol_wt=25758
     CDS             1..217
                     /gene="LOC144096759"
                     /coded_by="XM_077629634.1:53..706"
                     /db_xref="GeneID:144096759"
ORIGIN      
        1 mepsevtser epllpnepcv asmitpprsl gksksgriln sdsrnmifhc ytfwrnrepe
       61 rsveetgkfv admlgvsert vfrvreevkp shfsggkltt psrkrpcnae krrrrakfds
      121 ftlcalrscv hdffrrneip tvekittefs ermklpslrw wtvrrllaev gfkhekrsrn
      181 sllidrddit dwrnhylrdv eryreegrki fyldetw
//
LOCUS       XP_077485790             430 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096777 [Amblyomma americanum].
ACCESSION   XP_077485790
VERSION     XP_077485790.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629664.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..430
                     /product="uncharacterized protein LOC144096777"
                     /calculated_mol_wt=49188
     Region          213..374
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..430
                     /gene="LOC144096777"
                     /coded_by="XM_077629664.1:99..1391"
                     /db_xref="GeneID:144096777"
ORIGIN      
        1 maafgeaavd aarteyervl gyylcararr qrqeqqlddd feeaettfry ledqlmannf
       61 vilivlaeyy psthrerrry vrsdtwfedt lpnlsefhfr qalrlspstf ryivescrpv
      121 lerqttnmmt pisvqkrvav glyrlcssae drtiahlfgi grstvnvlhq efcvavvdll
      181 egewlqmihp nkmsahmref yaftgfpqai galdgchfpv sppkehatdy ynykgwysii
      241 llavvdhkyh fryinvgspg rchdasvygr sklhtlieng tfsspmamie gtsvppillc
      301 dqafplspnl qkpfpnaqpg sseavfnynl sktrriveng fgrlkarfrf vmkrmecrln
      361 kaklairaac vhhniceamk dsvepqwese araldlyaqp srntgecsgg gqevrhalan
      421 yfwkeaqhap
//
LOCUS       XP_077485796             126 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096785 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077485796
VERSION     XP_077485796.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629670.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..126
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..126
                     /product="uncharacterized protein LOC144096785 isoform X1"
                     /calculated_mol_wt=14145
     Region          38..119
                     /region_name="GRX_GRXh_1_2_like"
                     /note="Glutaredoxin (GRX) family, GRX human class 1 and 2
                     (h_1_2)-like subfamily; composed of proteins similar to
                     human GRXs, approximately 10 kDa in size, and proteins
                     containing a GRX or GRX-like domain. GRX is a glutathione
                     (GSH) dependent reductase; cd03419"
                     /db_xref="CDD:239511"
     Site            order(44,47,49,89..92,103..106)
                     /site_type="other"
                     /note="GSH binding site [chemical binding]"
                     /db_xref="CDD:239511"
     Site            order(47,50)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:239511"
     CDS             1..126
                     /gene="LOC144096785"
                     /coded_by="XM_077629670.1:158..538"
                     /db_xref="GeneID:144096785"
ORIGIN      
        1 mrislhrqmf qrlwkkqsaa agmsaatdfi eqtleknpvv ifsksycpfc hkakrvfdel
       61 qvpylaveld gradggdiqe vlkqktgart vprvfvnkqc lgggsdvenm yreeklqkll
      121 qsngll
//
LOCUS       XP_077485800             125 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096785 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077485800
VERSION     XP_077485800.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629674.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..125
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..125
                     /product="uncharacterized protein LOC144096785 isoform X2"
                     /calculated_mol_wt=13964
     Region          37..118
                     /region_name="GRX_GRXh_1_2_like"
                     /note="Glutaredoxin (GRX) family, GRX human class 1 and 2
                     (h_1_2)-like subfamily; composed of proteins similar to
                     human GRXs, approximately 10 kDa in size, and proteins
                     containing a GRX or GRX-like domain. GRX is a glutathione
                     (GSH) dependent reductase; cd03419"
                     /db_xref="CDD:239511"
     Site            order(43,46,48,88..91,102..105)
                     /site_type="other"
                     /note="GSH binding site [chemical binding]"
                     /db_xref="CDD:239511"
     Site            order(46,49)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:239511"
     CDS             1..125
                     /gene="LOC144096785"
                     /coded_by="XM_077629674.1:96..473"
                     /db_xref="GeneID:144096785"
ORIGIN      
        1 manvlrrmfq rlwkkqsaaa gmsaatdfie qtleknpvvi fsksycpfch kakrvfdelq
       61 vpylaveldg radggdiqev lkqktgartv prvfvnkqcl gggsdvenmy reeklqkllq
      121 sngll
//
LOCUS       XP_077485804             118 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096785 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077485804
VERSION     XP_077485804.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629678.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..118
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..118
                     /product="uncharacterized protein LOC144096785 isoform X3"
                     /calculated_mol_wt=13123
     Region          30..111
                     /region_name="GRX_GRXh_1_2_like"
                     /note="Glutaredoxin (GRX) family, GRX human class 1 and 2
                     (h_1_2)-like subfamily; composed of proteins similar to
                     human GRXs, approximately 10 kDa in size, and proteins
                     containing a GRX or GRX-like domain. GRX is a glutathione
                     (GSH) dependent reductase; cd03419"
                     /db_xref="CDD:239511"
     Site            order(36,39,41,81..84,95..98)
                     /site_type="other"
                     /note="GSH binding site [chemical binding]"
                     /db_xref="CDD:239511"
     Site            order(39,42)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:239511"
     CDS             1..118
                     /gene="LOC144096785"
                     /coded_by="XM_077629678.1:144..500"
                     /db_xref="GeneID:144096785"
ORIGIN      
        1 mfqrlwkkqs aaagmsaatd fieqtleknp vvifsksycp fchkakrvfd elqvpylave
       61 ldgradggdi qevlkqktga rtvprvfvnk qclgggsdve nmyreeklqk llqsngll
//
LOCUS       XP_077485815             153 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096820 [Amblyomma americanum].
ACCESSION   XP_077485815
VERSION     XP_077485815.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629689.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..153
                     /product="uncharacterized protein LOC144096820"
                     /calculated_mol_wt=17878
     Region          91..>153
                     /region_name="BTB_POZ_ZBTB_KLHL-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in zinc finger and
                     BTB domain-containing (ZBTB) proteins, Kelch-like (KLHL)
                     proteins, and similar proteins; cd18186"
                     /db_xref="CDD:349497"
     CDS             1..153
                     /gene="LOC144096820"
                     /coded_by="XM_077629689.1:42..503"
                     /db_xref="GeneID:144096820"
ORIGIN      
        1 mvllkmymdg ftyshiseaa tapistqyts fklnelfitf pdilteykvc yrcykrtnfw
       61 hwtsstcviv stqtetvpef slwrylgtre ladveivvhw ehapeqhasf kahrmilalq
      121 nvvframffg hfaredrlti mdmhpdgveg llr
//
LOCUS       XP_077485823             299 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096837 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077485823
VERSION     XP_077485823.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629697.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..299
                     /product="uncharacterized protein LOC144096837 isoform X1"
                     /calculated_mol_wt=33303
     Region          237..>299
                     /region_name="BTB_POZ_ZBTB_KLHL-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in zinc finger and
                     BTB domain-containing (ZBTB) proteins, Kelch-like (KLHL)
                     proteins, and similar proteins; cd18186"
                     /db_xref="CDD:349497"
     CDS             1..299
                     /gene="LOC144096837"
                     /coded_by="XM_077629697.1:36..935"
                     /db_xref="GeneID:144096837"
ORIGIN      
        1 mrhpqamcvh tvppdqsaaq thqigcrlql amqndlgqga ascrescrni atcatrpsgq
       61 ppdfelskcf vasgprstig gqaagdhhhl rgkgeevlkl reapaqkytq patavsrspq
      121 pagiklwvgq rqsavvslsp aatvqamvll kmymdgftys hiseaatapi stqytsfnlk
      181 elfitflnil teykvcyrcy krtnfwhwts stsvivstqt etvpefslwr ylgtreladv
      241 eivvhwehap eqhasfkahr milalqnvvf ramffghfar edrltimdmh pdgvegllr
//
LOCUS       XP_077485824             214 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096837 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077485824
VERSION     XP_077485824.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629698.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..214
                     /product="uncharacterized protein LOC144096837 isoform X2"
                     /calculated_mol_wt=24700
     Region          152..>214
                     /region_name="BTB_POZ_ZBTB_KLHL-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in zinc finger and
                     BTB domain-containing (ZBTB) proteins, Kelch-like (KLHL)
                     proteins, and similar proteins; cd18186"
                     /db_xref="CDD:349497"
     CDS             1..214
                     /gene="LOC144096837"
                     /coded_by="XM_077629698.1:558..1202"
                     /db_xref="GeneID:144096837"
ORIGIN      
        1 meivklmwrt gltmgshvfr almpdsssit srqewlprik lwvgqrqsav vslspaatvq
       61 amvllkmymd gftyshisea atapistqyt sfnlkelfit flnilteykv cyrcykrtnf
      121 whwtsstsvi vstqtetvpe fslwrylgtr eladveivvh wehapeqhas fkahrmilal
      181 qnvvframff ghfaredrlt imdmhpdgve gllr
//
LOCUS       XP_077485836             347 aa            linear   INV 25-NOV-2025
DEFINITION  BTB/POZ domain-containing protein 6-B-like [Amblyomma americanum].
ACCESSION   XP_077485836
VERSION     XP_077485836.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629710.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..347
                     /product="BTB/POZ domain-containing protein 6-B-like"
                     /calculated_mol_wt=39584
     Region          91..192
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     CDS             1..347
                     /gene="LOC144096863"
                     /coded_by="XM_077629710.1:55..1098"
                     /db_xref="GeneID:144096863"
ORIGIN      
        1 mvllkmymdg ftyshiseaa tapistqyts fnlnelfitf lnilteykvc yrcykrtnfw
       61 hwtsstrviv stqtetvpef slwrylgtre ladveivvhw ehapeqhasf kahrmilalq
      121 ndvyramffg hfaredrlti mdlhpdgveg llryfyseqf evesvhqatc trsaavkymv
      181 pelaarcvay verymepddv cpfldyiltm gedgvdgcgk avlhnnglfv lasktfescl
      241 hytanyildn vhnapemsvl qavhacgrrq ciergkvdge aaglrsavrp fflklrflvl
      301 tvtefvrgpn vwgmlnages lailcniiee dslpmpadfc tvrtqrv
//
LOCUS       XP_077485842             557 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096878 [Amblyomma americanum].
ACCESSION   XP_077485842
VERSION     XP_077485842.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629716.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..557
                     /product="uncharacterized protein LOC144096878"
                     /calculated_mol_wt=61872
     Region          <6..120
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            10
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238827"
     CDS             1..557
                     /gene="LOC144096878"
                     /coded_by="XM_077629716.1:15..1688"
                     /db_xref="GeneID:144096878"
ORIGIN      
        1 mmgtrgtpqg avlspllfni ammrlpsqla rvegiqhaly additiwtte gslgdmearl
       61 qqaasivday amdcglqcap aksellhvra npkdrtaihi slsggpirev eelrilglfi
      121 hhrlrpdsti vklkgigeqv grmirrvsnk rgglrgtdal rlvyafvtsr ilyavpylrt
      181 tkqdearida iirkatkral dlpvatsnak lkalgvlnsy qelreahlvn qytqlmqtap
      241 grrllnrlhi qhdctpeete rvpvlwrhml wdsplprnmd tdmhenrrea raralerqhg
      301 srpsvyyvdv awpsptgfyt atvvhqkmhv dglsfrapns mraeevaial aaahansrii
      361 itdsrkacdh ylageispla acilrrtatd stpkriiwap ghqgvrgnea adaaaraliy
      421 raphpsssgs etnqpllrfr eiithysdnh rlfpapakgl skteertlrr lqtgtllcpa
      481 ilkhydpntd grcphcgetc difhmvwact qnphlppspt psgeawetal lncsslesqr
      541 alvkrardga lscdvpd
//
LOCUS       XP_077485843             277 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096885 [Amblyomma americanum].
ACCESSION   XP_077485843
VERSION     XP_077485843.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629717.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..277
                     /product="uncharacterized protein LOC144096885"
                     /calculated_mol_wt=30722
     Region          <144..>215
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..277
                     /gene="LOC144096885"
                     /coded_by="XM_077629717.1:101..934"
                     /db_xref="GeneID:144096885"
ORIGIN      
        1 maahlaclll nayflavgas rsrrvvipgt trqpefpech ndvnnvvtac egelasllgr
       61 psmrnkrmtt snflsleevm efencilhrq eltlrefiaw cehksslakk vavcyrfvlk
      121 kskrhlsnsr iakglqkfqe cilnenggsd medgsetgid gdrtmppres tesdedkmdd
      181 sgtmhdpdee segspdrhpd edspsgkedd ddmkspnkai wtyplhdqsy yyprrekglg
      241 ngtvlpanqt agptvsqttg defdqlpvtk ppaasgr
//
LOCUS       XP_077485856             113 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096915 [Amblyomma americanum].
ACCESSION   XP_077485856
VERSION     XP_077485856.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629730.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..113
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..113
                     /product="uncharacterized protein LOC144096915"
                     /calculated_mol_wt=12893
     CDS             1..113
                     /gene="LOC144096915"
                     /coded_by="XM_077629730.1:84..425"
                     /db_xref="GeneID:144096915"
ORIGIN      
        1 melkfkerei adchsllekr vsrvsslpte lclvksamee qvrhhqaaem rimditlrey
       61 ssyegfqgfl kkldalntal seeikalrki ntllpnfldc tkkklkdaha nmg
//
LOCUS       XP_077485881             412 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096945 [Amblyomma americanum].
ACCESSION   XP_077485881
VERSION     XP_077485881.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629755.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..412
                     /product="uncharacterized protein LOC144096945"
                     /calculated_mol_wt=46023
     CDS             1..412
                     /gene="LOC144096945"
                     /coded_by="XM_077629755.1:28..1266"
                     /db_xref="GeneID:144096945"
ORIGIN      
        1 mqqdsivkpv rfltrkhvfp sniekmnvsr avqvfsppvt aalkllqeqa ghicdmsfaa
       61 vgptikfmdi mhrwfllmdv sncaqhiqqk qpdsrqydss gdsrlswlmp sfqsclqsmq
      121 rhcqakqfls ketyqalvmt ttsnvecikh llavesfkfv ltrkfssdpf esffgwlrrs
      181 agsndqtdar avlsgvekvq ktglvsasln snvvgtsnhs lgplstkpav tqhvdlfpqe
      241 trrllgdllk npksllptsd taalsmlggy larvvqekld cssctafltr passapsgsl
      301 ihqdrgglfy pstefvyvly alrkyievml ekrrfmekpl qaavstavdv laehgalkca
      361 rpcsdhhkel lklvctkffr pvitnyalsv tdrrdifkpl tskplsrkll kl
//
LOCUS       XP_077485893             195 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096956 [Amblyomma americanum].
ACCESSION   XP_077485893
VERSION     XP_077485893.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629767.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..195
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..195
                     /product="uncharacterized protein LOC144096956"
                     /calculated_mol_wt=23496
     Region          43..>195
                     /region_name="transpos_IS630"
                     /note="IS630 family transposase; NF033545"
                     /db_xref="CDD:468076"
     CDS             1..195
                     /gene="LOC144096956"
                     /coded_by="XM_077629767.1:1..588"
                     /db_xref="GeneID:144096956"
ORIGIN      
        1 mitppkslgk sksgrilnsd srnmifhcyt fwrnreperr veetskfvae mlgvsertvf
       61 rvrkevkash fsggklttps rkrprnaekr rrsakfdsft lyalrscvhd ffrrneiptv
      121 ekittefser mklpslrwwt vrrllaemgf khekrsrnsl lidrdditdw rnhylrdver
      181 yreegrkify ldetw
//
LOCUS       XP_077485908             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144096974 [Amblyomma americanum].
ACCESSION   XP_077485908
VERSION     XP_077485908.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629782.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144096974"
                     /calculated_mol_wt=25888
     Region          65..>217
                     /region_name="transpos_IS630"
                     /note="IS630 family transposase; NF033545"
                     /db_xref="CDD:468076"
     CDS             1..217
                     /gene="LOC144096974"
                     /coded_by="XM_077629782.1:198..851"
                     /db_xref="GeneID:144096974"
ORIGIN      
        1 mepsevtser epllpnepcv asmitpprsl gksksgriln sdsrnmifhc ytfwrnrepe
       61 rrveetskfv admlgvsert vfrvrkevka shfsggkltt psrkrprnae krrrsakfds
      121 ftlyalrscv hdffhrneip tvekittefs ermklpslrw wtvrrllaem gfkhekrsrn
      181 sllidrddit dwrnhylrdv eryreegrki fyldetw
//
LOCUS       XP_077485939             266 aa            linear   INV 25-NOV-2025
DEFINITION  dnaJ homolog subfamily B member 2-like [Amblyomma americanum].
ACCESSION   XP_077485939
VERSION     XP_077485939.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629813.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..266
                     /product="dnaJ homolog subfamily B member 2-like"
                     /calculated_mol_wt=29537
     Region          5..68
                     /region_name="DnaJ"
                     /note="DnaJ domain; pfam00226"
                     /db_xref="CDD:395170"
     Site            order(33..35,45,48..49,52..53)
                     /site_type="other"
                     /note="HSP70 interaction site [polypeptide binding]"
                     /db_xref="CDD:99751"
     CDS             1..266
                     /gene="LOC144097015"
                     /coded_by="XM_077629813.1:166..966"
                     /db_xref="GeneID:144097015"
ORIGIN      
        1 mpstdyysll evprsatldd iqrayrrlal kwhpdknpgn kdvaefrfke iseayqvlsd
       61 pskrkfydly gnggtsgerp stftflnptq lfckffgssd pfgelvrdlh avsgggggyt
      121 gptfttntfs sqqlsmafsf smnveyltln apvpvpwdtp ladvfqlyrn nfpppaaetv
      181 tevttvryed gkwvetctin kdntaatlry engqlvsctv eplvdphand dvedmqvdee
      241 eaevpeirlp kqlgpsddee gpstta
//
LOCUS       XP_077485956             266 aa            linear   INV 25-NOV-2025
DEFINITION  dnaJ homolog subfamily B member 2-like [Amblyomma americanum].
ACCESSION   XP_077485956
VERSION     XP_077485956.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629830.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..266
                     /product="dnaJ homolog subfamily B member 2-like"
                     /calculated_mol_wt=29292
     Region          5..>148
                     /region_name="PRK14292"
                     /note="chaperone protein DnaJ; Provisional"
                     /db_xref="CDD:237662"
     Region          5..68
                     /region_name="DnaJ"
                     /note="DnaJ domain; pfam00226"
                     /db_xref="CDD:395170"
     Site            order(33..35,45,48..49,52..53)
                     /site_type="other"
                     /note="HSP70 interaction site [polypeptide binding]"
                     /db_xref="CDD:99751"
     CDS             1..266
                     /gene="LOC144097038"
                     /coded_by="XM_077629830.1:208..1008"
                     /db_xref="GeneID:144097038"
ORIGIN      
        1 mpstdyysll evprsatldd iqsayrrlal kwhpdknpgn kdvaefrfke iseayqvlsd
       61 pskrkfydly gnggtsgerp stftflnptq lfckffgssd pfgelvrdvh avssggggyt
      121 gptfttntfs seqpnmafpf smnvehltlh apvpipwdtp ltdifqlyrn nfsppaaetv
      181 tevttvrnvd gkwvetctin kdntaatlcy edgqlvsctv eplvdphand dvedmqadqe
      241 eaavpeirlp kqlgpsddee gsstaa
//
LOCUS       XP_077485966             211 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097049 [Amblyomma americanum].
ACCESSION   XP_077485966
VERSION     XP_077485966.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629840.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..211
                     /product="uncharacterized protein LOC144097049"
                     /calculated_mol_wt=23348
     Region          94..175
                     /region_name="HSP20"
                     /note="Hsp20/alpha crystallin family; pfam00011"
                     /db_xref="CDD:459629"
     CDS             1..211
                     /gene="LOC144097049"
                     /coded_by="XM_077629840.1:298..933"
                     /db_xref="GeneID:144097049"
ORIGIN      
        1 mepaaksqlv vpvnfdsews tpgglevrvd evhsrfesyq vadaddcadl daewldfard
       61 hpdcvdnlga fldpdfeafr rvdgdpcgay eakhldlagy kpesvtvrvr egvvwvtase
      121 sekmcvvdns avkswrkler kfklpdgmpp eavctefmvd grlkislvpq spqhkryaag
      181 endsvstttt wngsdwtdve tdgskssivd r
//
LOCUS       XP_077485977             571 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier family 22 member 6-B-like [Amblyomma americanum].
ACCESSION   XP_077485977
VERSION     XP_077485977.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629851.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..571
                     /product="solute carrier family 22 member 6-B-like"
                     /calculated_mol_wt=62404
     Region          8..501
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..571
                     /gene="LOC144097060"
                     /coded_by="XM_077629851.1:1..1716"
                     /db_xref="GeneID:144097060"
ORIGIN      
        1 mevtnfiget gnyqnvilvl amyrgvmlav nnlcasfmfp dvghwcarlp yyanwsleew
       61 kdeaipqkss geyhtclrya vskhginrsa gvfcnaweyd hsefwpsgte kwnlvcesaw
      121 mnalpqsiym igmtvgfviv grisdmygrr pvllcglvsy vileytmavv gsftvfcslr
      181 ffnaiavaaa nnsltlyves igpsyrgrsm vayglfwgvg iallaglsai vpgwqwqltv
      241 yatmyalplv lwryieespk wlltvgkyar aeeairkiak ingrpemkee efaalkcyyr
      301 eqrssqeeis gsglkvlcks kkmilftvtn vffqfctaiv ryelaldtqm lpvdpytnfv
      361 iggvvevtsg iasyaillyl prkrsticfl llttaayvvh acvpkeyiaa etltmlvgrl
      421 cignvinini iylsemfptg aralaigfaq tvfgvgaaiq plikhpfgnp tadavfyavt
      481 mllataavla wpetkdrplp dfvanieaag ssqsssrsld stghielasf qapthaidvs
      541 savqragava aavaspagag vvaagqsets l
//
LOCUS       XP_077485986             224 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097071 [Amblyomma americanum].
ACCESSION   XP_077485986
VERSION     XP_077485986.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629860.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..224
                     /product="uncharacterized protein LOC144097071"
                     /calculated_mol_wt=25353
     CDS             1..224
                     /gene="LOC144097071"
                     /coded_by="XM_077629860.1:112..786"
                     /db_xref="GeneID:144097071"
ORIGIN      
        1 makvrdedvk nagkvvsepn mtyvqenpel ralidtvskt rpqegdstng lllgqyetvf
       61 pdfkrslqrl pstlrrtpgd rsldlddvne vkigiekrfe eetidndrgf trkidgeqrr
      121 rfaenqckks sfqdnssmpg tgaslpplhl gtlrrvkmsk krtiqgktti nrtttrkeee
      181 rsmtsptgei dcrrnvdesg ttaentethe eelvetyddd dtpl
//
LOCUS       XP_077486012             710 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097094 [Amblyomma americanum].
ACCESSION   XP_077486012
VERSION     XP_077486012.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629886.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..710
                     /product="uncharacterized protein LOC144097094"
                     /calculated_mol_wt=77941
     Region          <252..467
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     CDS             1..710
                     /gene="LOC144097094"
                     /coded_by="XM_077629886.1:80..2212"
                     /db_xref="GeneID:144097094"
ORIGIN      
        1 mqdeapnsga pddawerqld feracgadgp qqrcwlssdl tawnqvmhgf afelveakpg
       61 slrlqsiprg kaerdsaaaa reassllswl lghhrcveel avtcavhtgd iseeapcsir
      121 lrplpardaw rsirrleiee rpsaalvlrd vdavigvetl kinaraikpk faaeiegllr
      181 rnsssikavd isdmnrlprs vikaldclnn ceslaissli dfvrglpdmn svaqlvqtsk
      241 vlkelsvdpv mdeqisvtae alksnpaltk ltlfipdsti ppralfpale inttlkelrl
      301 tgccsmdals gqalasalrk ntclqelyie dvdidassmk dwpdalsent alecfqlsse
      361 qlpisgisal ckllpanktl kklvftgfqa aqeerlalae qlrndgcygr vqlpwteadl
      421 pgllsalgtp svcpgelhlp dicqlsedgl kllcdaltps krvrtlsaiv lgeplargta
      481 lcqmlranrs isclrlsmad dgagdfvrdv lhalavntai teidihldki drldtatafs
      541 ymlsrntaat nislwfsttf prqfireisr gmtlnklvvd lkfvteklcc dpsslvvfea
      601 lrrnraalnr avdfvlrrpa drrcaesfel fagrscllar lveitgktep evsldlsaae
      661 hflqdhylil tgiiqrtlvc rpaqatqlda lntdcwravv rhlkigdvra
//
LOCUS       XP_077486033             180 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097121 [Amblyomma americanum].
ACCESSION   XP_077486033
VERSION     XP_077486033.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629907.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..180
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..180
                     /product="uncharacterized protein LOC144097121"
                     /calculated_mol_wt=19030
     Region          <50..>146
                     /region_name="OATP"
                     /note="Organic Anion Transporter Polypeptide (OATP)
                     family; pfam03137"
                     /db_xref="CDD:460821"
     Region          <96..>143
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     CDS             1..180
                     /gene="LOC144097121"
                     /coded_by="XM_077629907.1:1241..1783"
                     /db_xref="GeneID:144097121"
ORIGIN      
        1 msvrvslaaa klkaidvaqk qekqqskqsr gthdaqelka aeapeagdda kehkgapspq
       61 qeapepkdvq spqtnagsgq tpevaersfv nhvrrlvfnp tfvcltlaaa aetmvasglt
      121 gfatkifiam fgisssqasg llgtfaahcs etssdaekml iyknmrtpdf ahfiqsaivl
//
LOCUS       XP_077486042             759 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097130 [Amblyomma americanum].
ACCESSION   XP_077486042
VERSION     XP_077486042.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629916.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..759
                     /product="uncharacterized protein LOC144097130"
                     /calculated_mol_wt=83673
     Region          <301..516
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     Region          <493..>605
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     CDS             1..759
                     /gene="LOC144097130"
                     /coded_by="XM_077629916.1:274..2553"
                     /db_xref="GeneID:144097130"
ORIGIN      
        1 myflryicar spprnsgpdd ssclkatpll eeedvdplrl qppigppgkm qdeapnsgap
       61 ddawerqldf eracgadgpq krcwlssdlt awnkvmhgfa felveakpgs lrlqsiprgk
      121 aerdsaaaar eassllswll ghhrcveela vtcavhtgdi seeapcsirl rpfpardawr
      181 sirrleieer psaalvlrdv davigvetlk inaraikpef aaeieallrr nsssikavdi
      241 sdmnrlprsv ikaldclnnc eslaisslid fvrglpdmns vaqlvqtskv lkelsvdpvm
      301 deqisvtaea lktnpaltkl tlfipdstip pralfpalei nttlkelrlt gccsmdalsg
      361 qalasalrkn tclrelyied vdidassmkd wpdalsenta lecfqlsseq vpisgisalc
      421 kllpanktlk klvfagfqaa qeerlalaeq lrydgcygrv qlpwteadlp gllsalatps
      481 vcpgelhlpd icqlsedglk llcdaltpsk rvrtlsamvl geplergtal cqmlranrsi
      541 sclrlsmadd gagdfvrdvf halavntait eidihldkid rldtatafsy mlsrnttatn
      601 islwfsttfp rqfireisrg mtlnklvvdl kfvteklccd psslvvfeal rrnraalnra
      661 vdfvlrrpad rrcaesfelf agrscllarl vevtgktepe vsldltaaeh flqdhylilt
      721 giiqrtlvcr paqatqldel ntdcwravvr hlkigdvra
//
LOCUS       XP_077486047             367 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097141 [Amblyomma americanum].
ACCESSION   XP_077486047
VERSION     XP_077486047.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629921.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..367
                     /product="uncharacterized protein LOC144097141"
                     /calculated_mol_wt=39021
     Region          30..113
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     CDS             1..367
                     /gene="LOC144097141"
                     /coded_by="XM_077629921.1:122..1225"
                     /db_xref="GeneID:144097141"
ORIGIN      
        1 merpqfcvkw khhqsnmlae fwqllsneal vdvtlacegl slkahkvvls acspffqalf
       61 venpcehpiv ilpamryidl kaivefmyrg evnvpldqia allktaetlk vkglaectne
      121 nkngglvtsv dgaadagpvg tprrasnvaa ssstavpqds igddveegdd adfdleildv
      181 meeftttenv pefpdvasss qvlgyesqqs hhtgftsdns alvpaqrslp sdfsitsqvd
      241 iepspyslip yddqaippvv aafpaaslsg dgasiamavt dasgvpavag psgyqqstps
      301 hgtypvpdhp thlhlfflrh vfptvilasa vlrgsalpat pgavhmlrlh raplnaglwl
      361 scpvfgl
//
LOCUS       XP_077486063             533 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097183 [Amblyomma americanum].
ACCESSION   XP_077486063
VERSION     XP_077486063.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629937.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..533
                     /product="uncharacterized protein LOC144097183"
                     /calculated_mol_wt=58872
     Region          33..79
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(33,36,48,50,53,56,67,70)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          <167..>391
                     /region_name="COG4913"
                     /note="Uncharacterized conserved protein, contains a
                     C-terminal ATPase domain [Function unknown]"
                     /db_xref="CDD:443941"
     CDS             1..533
                     /gene="LOC144097183"
                     /coded_by="XM_077629937.1:60..1661"
                     /db_xref="GeneID:144097183"
ORIGIN      
        1 mapasaqtvf gfgtgldwrp tcfvspfpss rvcsacclvp ssaamlacrh llcqscydga
       61 sakrshcpld kepfqeddva wttigrdgil grkvrcwnae hgcdnegvas amlehftnac
      121 qfhavsctrc seevlhrdia ehlecdcvps cprerplddn fanafmevke algkilegna
      181 avrrkldsfe drfrseagst fatqststad aatvalenii pvlraqaeat lleyravqaa
      241 lnklseenav lhtkldllee hlymknamat lstivsdals tavlktaavc raetaaaldk
      301 rwgqapteir qamagsvrav kgvisrecqr nvlglkasiv eafrgdrrla dagatsseta
      361 eklteivdde lkamellvva slsstndfln ksvpyewtve dwtefctkap lsaqyftgnd
      421 grpsyhygyl ivprlclsds kvwlrvymie slfdrflkwp mekkikllfi npcdstrela
      481 iesaisqelt rplaeyrkkv vflgrsdkip vqkfgghvli gsnklrlrle fip
//
LOCUS       XP_077486081             583 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097200 [Amblyomma americanum].
ACCESSION   XP_077486081
VERSION     XP_077486081.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629955.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..583
                     /product="uncharacterized protein LOC144097200"
                     /calculated_mol_wt=64342
     Region          493..577
                     /region_name="Spaetzle"
                     /note="cl29083"
                     /db_xref="CDD:475150"
     CDS             1..583
                     /gene="LOC144097200"
                     /coded_by="XM_077629955.1:259..2010"
                     /db_xref="GeneID:144097200"
ORIGIN      
        1 masvivflav tcapscflas asdseevlps vryrdynsre nqpkahrntp efvkdlyslq
       61 sipvpgvdye flpvpvkfpg dyrnggsdnq edkdeasdsa ekrsykepap eksdvvsetd
      121 egagvqneet qdsakqdlpr kavfdssder ydsslprsds gsnqrgyesy pprlpppsel
      181 rprlesqfra sstypgrkdv eaprsgflgv sqtrlepqpt fsanggrgnd drslkptvef
      241 hfdipndkfh tdlaqgrfst fkevpssldt lvssdvsfln kmiadppipe nltrdgtdrs
      301 ynrggdrant mvhifpsiqq qpnrpaleay qgyrssgspa peryesspgs yedggnngrs
      361 dsgsrgysse rdypyraaas tvhikaskia netprakras ktsghrqtri spdfiekkpk
      421 psqarstshe avraasciek iaaqpfclnd ssyptkeisw alrqdtefer tfrqlssavs
      481 rgkgaarrla vrcpssvrpv hplrgkdvlg ewktivnvkg fsqevlldtc stekspcsar
      541 ppkscvqsyq vqrlalydeh arklysgefr vpagctcggd arv
//
LOCUS       XP_077486091             328 aa            linear   INV 25-NOV-2025
DEFINITION  glyoxylate reductase/hydroxypyruvate reductase-like [Amblyomma
            americanum].
ACCESSION   XP_077486091
VERSION     XP_077486091.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629965.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..328
                     /product="glyoxylate reductase/hydroxypyruvate
                     reductase-like"
                     /calculated_mol_wt=36053
     Region          8..318
                     /region_name="NADB_Rossmann"
                     /note="Rossmann-fold NAD(P)(+)-binding proteins; cl21454"
                     /db_xref="CDD:473865"
     CDS             1..328
                     /gene="LOC144097210"
                     /coded_by="XM_077629965.1:171..1157"
                     /db_xref="GeneID:144097210"
ORIGIN      
        1 maasgtfkvf vsrtdippra iqmletkcvv tsvpctplnw lemwpkyvhg mdalfiqhye
       61 qinaktldmv gprlqvvstm svhygnidld ecrkrgihvg ntqhvqndav aevalaltlm
      121 asrriaegid airagnwtpa tdlswlvgpe lvgkvagiig lggigmeiin rlrafkvrsf
      181 lyhnrrprld pmthdiqytt lkhllcnsdi vivccplnkd sqqmfnektf hlmkhtaifi
      241 sisrrevvne ealcqalqtg giafaaldaa gdtpmavdsp llklsncivt pnmaadgrdt
      301 kvktatmcae nilaalenky haaanllf
//
LOCUS       XP_077486103            1114 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097229 [Amblyomma americanum].
ACCESSION   XP_077486103
VERSION     XP_077486103.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629977.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1114
                     /product="uncharacterized protein LOC144097229"
                     /calculated_mol_wt=120774
     Region          <735..1113
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          845..>1028
                     /region_name="PRK12727"
                     /note="flagellar biosynthesis protein FlhF"
                     /db_xref="CDD:237182"
     CDS             1..1114
                     /gene="LOC144097229"
                     /coded_by="XM_077629977.1:96..3440"
                     /db_xref="GeneID:144097229"
ORIGIN      
        1 mspgglvlal llalmpsirt nftepdynas tvvssasead ietynveqes sspfmsdlgs
       61 divsrlahpp ydasileeaa anptydavke niayhqpsvg sfttflwavl vffcltslgn
      121 tvhraiacgt gefrhrvtps ymtffvfllv vlgilfvlmt llllclvstr dsmvdsvtek
      181 vpavlastfn glrgfvnltv aqialnrrta rmpkvgkvlg dgfdiafksf vassatadvr
      241 ipfyhgdqec andqryfgsm antrnngrmg dkfqpmenqc ahvhqdqeql fgdvsravaa
      301 atrsdrqkvy eilsdyasmm desdcnvlps hnsinqlekd vhafedskgp fkslasfves
      361 dlfwvvilhi sslcllivgv lmgvcvhnse tdphertrls hligceltlt ayaftlfviv
      421 aiwksmflvv gstlgytyfc epyltknyii lddalerlwp asnrshllsr impsevmesc
      481 qndsvsildl gpkpshgrts nrqgvflprl nlknvlrels sqprrgnmen slsryanffa
      541 nvrqssqnsl saparyvndn lsdnvdlder sglwysdkva mldktavega llpiipsyyn
      601 rfvkslvntv mqpkgssvag rcsllralft sfmdvvcyty vlefvaywas lllamllaiv
      661 tipfcfmlak yffrckrpir vlrraksesi ppshrarkrr sleseetsqy qresqqyqgh
      721 plarllqmys saelppgtsr rivamstkgt siippfgppm qqlssfshrl tqtprrlhav
      781 ssstsivtsg vpvlagipta smgamvpslm pgpfvgaaps hvasssvhre ssltmsqskg
      841 rsrfaasmas sshqqlapqa qlplmyaspa avampasqpv lvapqpmhav cvpvqasaps
      901 arvlqqttvv astsqlnlaa pppppvigvv atsssmpptt vypqvyraas qarpaqvava
      961 pamttpmhva pvrrttsvhr vssssafqtm ptlvpptsav mparavmpar vavapamaap
     1021 mhvaparrtt svhrvssssg ggfqtmtglv aptsgvvpas tvvparsvmp vggvvpvrsa
     1081 vparsvvhsg svvhvypqrt lsahsigthg rhhp
//
LOCUS       XP_077486112             522 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097239 [Amblyomma americanum].
ACCESSION   XP_077486112
VERSION     XP_077486112.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629986.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..522
                     /product="uncharacterized protein LOC144097239"
                     /calculated_mol_wt=58823
     Region          52..>159
                     /region_name="SWIM"
                     /note="Uncharacterized protein, contains SWIM-type Zn
                     finger domain [Function unknown]; COG4715"
                     /db_xref="CDD:443750"
     Region          269..469
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(323,362,375,377)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..522
                     /gene="LOC144097239"
                     /coded_by="XM_077629986.1:1..1569"
                     /db_xref="GeneID:144097239"
ORIGIN      
        1 mieeenipfp fqetgwredf srcsvrtehv wqflhsatst vrqahrdwsf keegyvknlk
       61 vnlgtaddel glmraacaps mktgvyvtta wfvvgtghiv gahcdcaagl sescqhvagl
      121 lfsaaaraed apsctdvlck wivpaeakka aprkplveik fqkycvnkpp rgkrerdyap
      181 cpitplptph dieslrkkla sacpdlqalr ylcptdkakt rppcnkkaie ysdglwseps
      241 atevqsymqt lkllsreerh diceetvgqa ankkwhaarv grltasllkr icrctkpdgl
      301 lkgllyrcnr atseaiaygr eheadavkay vklqhakdls vavhetgllv hnqysfiaas
      361 pdrivvldge egllevkcpf skkdmaceea cldknfccrl tedgvelkrd hayfyqiqgq
      421 mavtghnwcd lviwtagdtp edppylyver iefneefwnr ellpgllhfs khalipeilt
      481 rrvkrlgrly tsgtyvshsk frqgyyvcip lgglrikmkk lk
//
LOCUS       XP_077486121             116 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097249 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077486121
VERSION     XP_077486121.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077629995.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..116
                     /product="uncharacterized protein LOC144097249 isoform X1"
                     /calculated_mol_wt=12583
     CDS             1..116
                     /gene="LOC144097249"
                     /coded_by="XM_077629995.1:53..403"
                     /db_xref="GeneID:144097249"
ORIGIN      
        1 maatlaqlya mlmleaavla pvepsalett wwndtcemgp siiqplleqp vvvtgcvsrn
       61 ishqsevklp liihftghlv lntcsglhct flrsllyhla salqllsacw rsaiqn
//
LOCUS       XP_077486127              70 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097249 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077486127
VERSION     XP_077486127.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630001.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..70
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..70
                     /product="uncharacterized protein LOC144097249 isoform X2"
                     /calculated_mol_wt=7524
     CDS             1..70
                     /gene="LOC144097249"
                     /coded_by="XM_077630001.1:53..265"
                     /db_xref="GeneID:144097249"
ORIGIN      
        1 maatlaqlya mlmleaavla pvepsalett wwndtcemgp siiqplleqp vvvtgcvsrn
       61 ishqseiwvv
//
LOCUS       XP_077486146             170 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077486146
VERSION     XP_077486146.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630020.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..170
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..170
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=19000
     Region          <7..126
                     /region_name="Piwi-like"
                     /note="PIWI domain. Domain found in proteins involved in
                     RNA silencing. RNA silencing refers to a group of related
                     gene-silencing mechanisms mediated by short RNA molecules,
                     including siRNAs, miRNAs, and heterochromatin-related
                     guide RNAs. The central component...; cl00628"
                     /db_xref="CDD:412485"
     CDS             1..170
                     /gene="LOC144097273"
                     /coded_by="XM_077630020.1:23..535"
                     /db_xref="GeneID:144097273"
ORIGIN      
        1 mcptetyepp ltfivvrkrh htrfmpandr dgvgkfrnvp pgttvdsvvt hpldfdfflc
       61 shfgiqgtsr pahyyivwdd skfsaddlqk lsfylchtys rcarsvsipa pvyyahlaaf
      121 rakqhiaskl essgavsqss eggwdavltt aqyveavkvl qelqasmyfv
//
LOCUS       XP_077486157             178 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097283 [Amblyomma americanum].
ACCESSION   XP_077486157
VERSION     XP_077486157.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630031.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..178
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..178
                     /product="uncharacterized protein LOC144097283"
                     /calculated_mol_wt=20289
     CDS             1..178
                     /gene="LOC144097283"
                     /coded_by="XM_077630031.1:57..593"
                     /db_xref="GeneID:144097283"
ORIGIN      
        1 melsgvipsv ssmqmsppgs heaaaasgng trsqadhved agswemliqe fvrdlkdqsm
       61 reeplpdnsk kqgakdrvda ekekereeel pprrrrkrra ifkrlgsvgr pryrakvkep
      121 tfpchrcpar frfmhelech iaehtgrplp llpcrfcple fktgrmlssh isrkhgrp
//
LOCUS       XP_077486177             146 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097307 [Amblyomma americanum].
ACCESSION   XP_077486177
VERSION     XP_077486177.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630051.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..146
                     /product="uncharacterized protein LOC144097307"
                     /calculated_mol_wt=15833
     CDS             1..146
                     /gene="LOC144097307"
                     /coded_by="XM_077630051.1:282..722"
                     /db_xref="GeneID:144097307"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrppfa altsvlccqr pasigpcvlp afgcvippdp
       61 fmlktkscme vvqatklrvi fardleqagk lvdgqwysls kasffsktkn iflgswamyl
      121 gtcgqdnagq hasasskkkh etvnky
//
LOCUS       XP_077486186             600 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077486186
VERSION     XP_077486186.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630060.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..600
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=67431
     Region          <1..25
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; cl02747"
                     /db_xref="CDD:470663"
     Region          127..494
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..600
                     /gene="LOC144097317"
                     /coded_by="XM_077630060.1:25..1827"
                     /db_xref="GeneID:144097317"
ORIGIN      
        1 myflkfrigi cvpstcsldd vrslaryfgk qmktnvtvpw cevkqkvvlp dyyiailsif
       61 gcgvliviag tlseivpkir rvirrepepl kepekgvprq lleafsaysn grkilstktn
      121 drsmrclhgi rfismlwvml ghvyfsrnns ivsgairakd lgkdvafqvv lnaslsvdsf
      181 fllsglllmf vcygklkqdk grfdflmyyi hryirltppf lmvsltlilg ppwmsgplwh
      241 etvdrfaegc krywwtnliy isswmktqev clphgwylsc dmqyfiiapi ffmalyrrpa
      301 lglgligafa fasmavtglv tyinddgpva lfsipddenl nsflndiafr pythfpsfai
      361 glylgyliyk yrqikisrri qlvgwlssvt tgltivygvy ewnaysppgp llsvlygats
      421 rpawalcvaw vtfacvsghg gfvntllswk afvplskmtf mvyllhpplm fmyaahtmna
      481 iyvtqfmmvf vyfahllacy maafvgcvtc etpfinveki lfnvlaeyrk srraepsstr
      541 avppprlqsp pvrpagvavv apvapegrtr pdpesaataa cggrvvatga ecarddscrl
//
LOCUS       XP_077486196             505 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097328 [Amblyomma americanum].
ACCESSION   XP_077486196
VERSION     XP_077486196.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630070.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..505
                     /product="uncharacterized protein LOC144097328"
                     /calculated_mol_wt=55122
     Region          339..434
                     /region_name="Plus-3"
                     /note="Plus-3 domain; pfam03126"
                     /db_xref="CDD:460817"
     CDS             1..505
                     /gene="LOC144097328"
                     /coded_by="XM_077630070.1:218..1735"
                     /db_xref="GeneID:144097328"
ORIGIN      
        1 mprrrhapld wssgesdcpd eldrqlrlla krrkvepets aqqsqpdgws qgrpsgatss
       61 asnraargqg saksgrsrls kkadrqdrss nagaedessa sseegdvdrd gsaeedsdgg
      121 lvgdqgyrar ravvteecre qeklvahagk rgrpkkrfgs knrprlakry eqtwesedgd
      181 ssassqgvea pstssgckgr hglakryeqt wesedgdssa ssqgeqgpsa ssgckrrrtf
      241 grrkgkkart trglkaarqk qlhkggdkrq kslaegevrl ktskrlqvsd vysdddgede
      301 sdggavggtt sergqstssf qpraqsgpep paaaakedig rmrltrnqlk vlihvptfps
      361 iavgcfvrvr tkrrtvyrlm evtavvetan vrslglartn kalrlrhgsr ekvrrlgcvs
      421 nqqftdrefq kwkrltiskg aafptpreva qkasdiqaal rdapvpsdge tdgneeergp
      481 lsvhnyaaqr pesgdekggr arrkp
//
LOCUS       XP_077486214             155 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097345 [Amblyomma americanum].
ACCESSION   XP_077486214
VERSION     XP_077486214.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630088.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..155
                     /product="uncharacterized protein LOC144097345"
                     /calculated_mol_wt=16920
     CDS             1..155
                     /gene="LOC144097345"
                     /coded_by="XM_077630088.1:272..739"
                     /db_xref="GeneID:144097345"
ORIGIN      
        1 mnrvtavlpa ilrarnssqa rdpthrprft altsvlcfql pasigpcvlp afdcviplep
       61 fmfvglwglq lldpsshcvp cqactfesgp gatlllsgcs avepfrssnq qfgdsaprpv
      121 qlvlhhrpyl qaswlslshl ytprqkttfl tswam
//
LOCUS       XP_077486225             296 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097358 [Amblyomma americanum].
ACCESSION   XP_077486225
VERSION     XP_077486225.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630099.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..296
                     /product="uncharacterized protein LOC144097358"
                     /calculated_mol_wt=31234
     Region          117..173
                     /region_name="bHLH_TS_NGN"
                     /note="basic helix-loop-helix (bHLH) domain found in
                     neurogenin (NGN) family; cd11428"
                     /db_xref="CDD:381434"
     Site            order(118,122,124..126,128,133,151..154)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381434"
     Site            order(131..132,135..136,138..139,155,158,162,164..165,169,
                     171..172)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:381434"
     CDS             1..296
                     /gene="LOC144097358"
                     /coded_by="XM_077630099.1:71..961"
                     /db_xref="GeneID:144097358"
ORIGIN      
        1 mtpamlslks eapmspqeda afrngvsgsf sedddlpddr sptsssqdll spcpssymda
       61 ddfddaesgd sssttaasqm ssrrkrapkp sarsrtaaan aaaglkapap pkmkktrrlk
      121 andrernrmh nlnsaldrlr cvlptfpddt kltkietlrf ahnyiwalse tlklveskae
      181 kkesqqvnqd slgtsmppag iqesttaspt apltpmaagg pttprgyytp plsvtsspss
      241 vrqltpsaac lgspstpwnl sscpgsvpis tpassvdfsd sscsgsecgf atyetl
//
LOCUS       XP_077486275             699 aa            linear   INV 25-NOV-2025
DEFINITION  neprilysin-1-like [Amblyomma americanum].
ACCESSION   XP_077486275
VERSION     XP_077486275.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630149.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..699
                     /product="neprilysin-1-like"
                     /calculated_mol_wt=79966
     Region          96..697
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..699
                     /gene="LOC144097428"
                     /coded_by="XM_077630149.1:45..2144"
                     /db_xref="GeneID:144097428"
ORIGIN      
        1 madqarrggf ampggrvrpi kailmaifsm clavaialvv vftvknihrp kfhgefqaah
       61 lfswqrqrsg fiytctsehc lkegeqlqkh vawtldpcdd fhdyvcsstk passvrksav
      121 khflleihdv idkysrlgwl krtkltsqis sfyrackrgr ytsvpgqmde mlqllklggk
      181 hahleeglak larilqvfpl vhvsvtnrak nsrecivlkr prrfdgdhif dipkafskky
      241 vafvkeligs ehaannvrei diflkkfsgl wsesyqkmse ydtttiedlt tegfdwktfl
      301 fnllesarkd dcvvirspey fkhlekmlqk ftnrqvrdyv eysailhlyp fvklrqasst
      361 payhnvsrev cslltyrvfn yvfvrhfnst vrldvitsag mkeailrnsy rsvldsavei
      421 pmttvnsied eimsiapsyf slmenvdeyy avnmyepdvk nmlasyvkak sslsehyief
      481 vlkagdtvqd gnleltsqfd pdtnvltvpl siaplvlkgr efflesstvg fvmtrflynv
      541 fqekflfspd adteallgvk ncleqqygsi evgpsnrtld gqrtlrenma dnvavtvlhr
      601 ayrtsllfyi stmdalgfqn vflhfenlps vsseqlfylm fgqsfcdpde dterviddat
      661 wapakyrvnv plsnyepfsk afeckpgsrm npkkkcral
//
LOCUS       XP_077486283             282 aa            linear   INV 25-NOV-2025
DEFINITION  SAP30-binding protein-like [Amblyomma americanum].
ACCESSION   XP_077486283
VERSION     XP_077486283.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630157.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..282
                     /product="SAP30-binding protein-like"
                     /calculated_mol_wt=31057
     Region          112..203
                     /region_name="HCNGP"
                     /note="HCNGP-like protein; pfam07818"
                     /db_xref="CDD:462281"
     CDS             1..282
                     /gene="LOC144097438"
                     /coded_by="XM_077630157.1:156..1004"
                     /db_xref="GeneID:144097438"
ORIGIN      
        1 mnsdssrkat aleslvetyt dsddeleaep qdnttplsae devqstesaa qgavslvsyg
       61 ledddgasgs sedeqpeese dgesskatrp rpespvsfhr rlknlpvdei vlppeppgrc
      121 apalqqkiar lyerkvrkgv dmnatiqmrk dfrnpsmyek lvsfcgidel gtnyppelyd
      181 psswgpesdy eevsrrqkee mekrqrekkp kveffrgvsk vpkptkskwd vvhrepfqgi
      241 prpsatttaa lgapiaiagt skstvisafg alskkpptdt kh
//
LOCUS       XP_077486294             198 aa            linear   INV 25-NOV-2025
DEFINITION  longitudinals lacking protein-like [Amblyomma americanum].
ACCESSION   XP_077486294
VERSION     XP_077486294.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630168.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..198
                     /product="longitudinals lacking protein-like"
                     /calculated_mol_wt=21516
     Region          30..112
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     CDS             1..198
                     /gene="LOC144097445"
                     /coded_by="XM_077630168.1:89..685"
                     /db_xref="GeneID:144097445"
ORIGIN      
        1 masqqfcvkw nshhsnmlaa feqllsneal vdvtlacegl slkahkivls acspffqaif
       61 venpckhpiv ilqdmryvdl kavvdfmyrg evnvsqdhia aflktaetlk vkiftevane
      121 nkngapvtsv dgngcrvdgd dadfdvehln vaeqsmtten vpvfpyvass pqalgdesqq
      181 shhtvstsdn salvlahp
//
LOCUS       XP_077486306             155 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097454 [Amblyomma americanum].
ACCESSION   XP_077486306
VERSION     XP_077486306.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630180.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..155
                     /product="uncharacterized protein LOC144097454"
                     /calculated_mol_wt=16265
     CDS             1..155
                     /gene="LOC144097454"
                     /coded_by="XM_077630180.1:104..571"
                     /db_xref="GeneID:144097454"
ORIGIN      
        1 maprltgvge kgtlkteaqi nsiesqnral pgwpmpvfas ssdytrrlkr ndeeersnpa
       61 srglgwrtsr aaaalspkrl ptparrdhdg pgasvlgrvt rseagshgpg gqrgftvarv
      121 trseagshgl gaqrsftvgr vttseagrhg sgapr
//
LOCUS       XP_077486315             155 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097463 [Amblyomma americanum].
ACCESSION   XP_077486315
VERSION     XP_077486315.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630189.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..155
                     /product="uncharacterized protein LOC144097463"
                     /calculated_mol_wt=16265
     CDS             1..155
                     /gene="LOC144097463"
                     /coded_by="XM_077630189.1:88..555"
                     /db_xref="GeneID:144097463"
ORIGIN      
        1 maprltgvge kgtlkteaqi nsiesqnral pgwpmpvfas ssdytrrlkr ndeeersnpa
       61 srglgwrtsr aaaalspkrl ptparrdhdg pgasvlgrvt rseagshgpg gqrgftvarv
      121 trseagshgl gaqrsftvgr vttseagrhg sgapr
//
LOCUS       XP_077486328             122 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097483 [Amblyomma americanum].
ACCESSION   XP_077486328
VERSION     XP_077486328.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630202.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..122
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..122
                     /product="uncharacterized protein LOC144097483"
                     /calculated_mol_wt=12850
     CDS             1..122
                     /gene="LOC144097483"
                     /coded_by="XM_077630202.1:182..550"
                     /db_xref="GeneID:144097483"
ORIGIN      
        1 msatnirrpa sqasmgsgpg ssgsgsriev stvqvnqddq tgnltvyggg qysirttisn
       61 yqiadqtgqv rninvpapqv tpgqpgkfek riivengiet veeyqndrlv srtvngvpqa
      121 ls
//
LOCUS       XP_077486331             193 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097490 [Amblyomma americanum].
ACCESSION   XP_077486331
VERSION     XP_077486331.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630205.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..193
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..193
                     /product="uncharacterized protein LOC144097490"
                     /calculated_mol_wt=20224
     CDS             1..193
                     /gene="LOC144097490"
                     /coded_by="XM_077630205.1:483..1064"
                     /db_xref="GeneID:144097490"
ORIGIN      
        1 mssessssps nassrldvst elgggprvev savrtgsrqd qqggasasvd qtpgvrtsis
       61 qaridygvcp aprgasiapn sfyapydpyn hyspynpynp yilcspccvp cttychqapv
      121 vasapqgpav svtlgsavdg tastapgpar lpelesrekr iiikngfevv eeyvndrlvs
      181 rtvnrvpast srq
//
LOCUS       XP_077486340             136 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097500 [Amblyomma americanum].
ACCESSION   XP_077486340
VERSION     XP_077486340.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630214.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..136
                     /product="uncharacterized protein LOC144097500"
                     /calculated_mol_wt=14983
     CDS             1..136
                     /gene="LOC144097500"
                     /coded_by="XM_077630214.1:293..703"
                     /db_xref="GeneID:144097500"
ORIGIN      
        1 msnsslcvhe thirpvigvi qqqgkddepr ievgevtlgt dnvlagsltv ygggqyhikt
       61 tvsdevanye itdekgqtrn ilvavpkppv ekikferrii lengvetiee yqnnkmvrri
      121 vdgnevpvad kgddkk
//
LOCUS       XP_077486346             250 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097512 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077486346
VERSION     XP_077486346.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630220.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..250
                     /product="uncharacterized protein LOC144097512 isoform X1"
                     /calculated_mol_wt=27980
     Region          3..66
                     /region_name="DnaJ"
                     /note="DnaJ domain; pfam00226"
                     /db_xref="CDD:395170"
     Site            order(31..33,43,46..47,50..51)
                     /site_type="other"
                     /note="HSP70 interaction site [polypeptide binding]"
                     /db_xref="CDD:99751"
     CDS             1..250
                     /gene="LOC144097512"
                     /coded_by="XM_077630220.1:140..892"
                     /db_xref="GeneID:144097512"
ORIGIN      
        1 mldyyrilgv pqtappeeik kayrrlclrw hpdknpddke kaeqnfrsva qayqtlsdeq
       61 krkdydyqca ilrararnqa rrqaatspfv tleeiikgip rrsweapaad sagatassyp
      121 ptpfaackps gsrrrggfha girleagtdg frmihhctms rgavpmatrl tqtsiamhkp
      181 rpvpvlgphr paippvsrpr lfevrtvicd gvqricryed gvrvsavthp vphlrqpsir
      241 plvlthrpgl
//
LOCUS       XP_077486350             250 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097512 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077486350
VERSION     XP_077486350.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630224.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..250
                     /product="uncharacterized protein LOC144097512 isoform X1"
                     /calculated_mol_wt=27980
     Region          3..66
                     /region_name="DnaJ"
                     /note="DnaJ domain; pfam00226"
                     /db_xref="CDD:395170"
     Site            order(31..33,43,46..47,50..51)
                     /site_type="other"
                     /note="HSP70 interaction site [polypeptide binding]"
                     /db_xref="CDD:99751"
     CDS             1..250
                     /gene="LOC144097512"
                     /coded_by="XM_077630224.1:105..857"
                     /db_xref="GeneID:144097512"
ORIGIN      
        1 mldyyrilgv pqtappeeik kayrrlclrw hpdknpddke kaeqnfrsva qayqtlsdeq
       61 krkdydyqca ilrararnqa rrqaatspfv tleeiikgip rrsweapaad sagatassyp
      121 ptpfaackps gsrrrggfha girleagtdg frmihhctms rgavpmatrl tqtsiamhkp
      181 rpvpvlgphr paippvsrpr lfevrtvicd gvqricryed gvrvsavthp vphlrqpsir
      241 plvlthrpgl
//
LOCUS       XP_077486355             216 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097512 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077486355
VERSION     XP_077486355.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630229.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..216
                     /product="uncharacterized protein LOC144097512 isoform X2"
                     /calculated_mol_wt=24135
     Region          3..66
                     /region_name="DnaJ"
                     /note="DnaJ domain; pfam00226"
                     /db_xref="CDD:395170"
     Site            order(31..33,43,46..47,50..51)
                     /site_type="other"
                     /note="HSP70 interaction site [polypeptide binding]"
                     /db_xref="CDD:99751"
     CDS             1..216
                     /gene="LOC144097512"
                     /coded_by="XM_077630229.1:140..790"
                     /db_xref="GeneID:144097512"
ORIGIN      
        1 mldyyrilgv pqtappeeik kayrrlclrw hpdknpddke kaeqnfrsva qayqtlsdeq
       61 krkdydyqsw eapaadsaga tassypptpf aackpsgsrr rggfhagirl eagtdgfrmi
      121 hhctmsrgav pmatrltqts iamhkprpvp vlgphrpaip pvsrprlfev rtvicdgvqr
      181 icryedgvrv savthpvphl rqpsirplvl thrpgl
//
LOCUS       XP_077486364             254 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097538 [Amblyomma americanum].
ACCESSION   XP_077486364
VERSION     XP_077486364.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630238.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..254
                     /product="uncharacterized protein LOC144097538"
                     /calculated_mol_wt=28513
     Region          6..69
                     /region_name="DnaJ"
                     /note="DnaJ domain; pfam00226"
                     /db_xref="CDD:395170"
     Site            order(34..36,46,49..50,53..54)
                     /site_type="other"
                     /note="HSP70 interaction site [polypeptide binding]"
                     /db_xref="CDD:99751"
     CDS             1..254
                     /gene="LOC144097538"
                     /coded_by="XM_077630238.1:141..905"
                     /db_xref="GeneID:144097538"
ORIGIN      
        1 maqrpdyyai lgltrdataa ditkayktla lqwhpdknvd nkkkaermfr niseahsvlg
       61 nessrqryde rlrrasipps psttsrtycy ivpshprqss aqqscsytqa pvtaypqssv
      121 atypnysmtt vysppsagrh paysysmyqp ststqypytv ppsnyvpkpa msmttyiptv
      181 saptpyyqtp stttnvsilr lptdyerqts trivqgnrmd levikenaae ivnvyengvl
      241 trstyrrtgp ppyl
//
LOCUS       XP_077486367             258 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097550 [Amblyomma americanum].
ACCESSION   XP_077486367
VERSION     XP_077486367.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630241.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..258
                     /product="uncharacterized protein LOC144097550"
                     /calculated_mol_wt=28387
     CDS             1..258
                     /gene="LOC144097550"
                     /coded_by="XM_077630241.1:103..879"
                     /db_xref="GeneID:144097550"
ORIGIN      
        1 madgelgahc sdssrslpcf svdetvlnil wdeeiqmedl lefsfdridk laagrgktvt
       61 vdekerlwdy iermrgekde witkeslpwd eineryalrs kslsrmsipn lflpeepapg
      121 ketkkqalce lqagesltqs evwdvgsprg hdipamkkas akkehgwkra lknvlkddak
      181 keagilrlgg ldefatgitm tssplpvpra ltadgppppp aatfdeerpa glirqslssf
      241 gmhsgkkdaa gptkadnv
//
LOCUS       XP_077486368             109 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097563 [Amblyomma americanum].
ACCESSION   XP_077486368
VERSION     XP_077486368.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630242.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..109
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..109
                     /product="uncharacterized protein LOC144097563"
                     /calculated_mol_wt=12676
     CDS             1..109
                     /gene="LOC144097563"
                     /coded_by="XM_077630242.1:73..402"
                     /db_xref="GeneID:144097563"
ORIGIN      
        1 megtpegndg lgacradsqr slscfsvden vlhvlwdeei rmeellgfsf ehidklaarr
       61 gktvtmeeke rlwdyvermr seqdewvtkd svswdevdar yalrtksfr
//
LOCUS       XP_077486372             111 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097570 [Amblyomma americanum].
ACCESSION   XP_077486372
VERSION     XP_077486372.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630246.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..111
                     /product="uncharacterized protein LOC144097570"
                     /calculated_mol_wt=12037
     CDS             1..111
                     /gene="LOC144097570"
                     /coded_by="XM_077630246.1:55..390"
                     /db_xref="GeneID:144097570"
ORIGIN      
        1 mctglkdttt vkypgplpvq epaprpssap prlgtatsqq talplgvgrs reqhqlqaga
       61 qpqpqlqpqw qfvawphrna gsfyvlhvps lqdcaqqdtq pshgrrwpgd n
//
LOCUS       XP_077486379             198 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097580 [Amblyomma americanum].
ACCESSION   XP_077486379
VERSION     XP_077486379.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630253.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..198
                     /product="uncharacterized protein LOC144097580"
                     /calculated_mol_wt=21732
     Region          <2..176
                     /region_name="COG5025"
                     /note="Transcription factor of the Forkhead/HNF3 family
                     [Transcription]"
                     /db_xref="CDD:227358"
     Region          28..102
                     /region_name="Forkhead"
                     /note="Forkhead domain; pfam00250"
                     /db_xref="CDD:459732"
     CDS             1..198
                     /gene="LOC144097580"
                     /coded_by="XM_077630253.1:63..659"
                     /db_xref="GeneID:144097580"
ORIGIN      
        1 mappgplgel lpmgtclrrp sgflvqhian aihksprrml rvshiyealr nkfpfyrfvd
       61 vdaqatwkss vrhalyqnwf ikvpcsrakn sfwslnyser prdwimpeke eqgqplette
      121 altlasdlgl vpsaartppn lesprdirms svptagasql pvvsslsqee sstsnspqtv
      181 gtaaavdagk aevvslef
//
LOCUS       XP_077486388             111 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097591 [Amblyomma americanum].
ACCESSION   XP_077486388
VERSION     XP_077486388.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630262.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..111
                     /product="uncharacterized protein LOC144097591"
                     /calculated_mol_wt=12037
     CDS             1..111
                     /gene="LOC144097591"
                     /coded_by="XM_077630262.1:26..361"
                     /db_xref="GeneID:144097591"
ORIGIN      
        1 mctglkdttt vkypgplpvq epaprpssap prlgtatsqq talplgvgrs reqhqlqaga
       61 qpqpqlqpqw qfvawphrna gsfyvlhvps lqdcaqqdtq pshgrrwpgd n
//
LOCUS       XP_077486399             198 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097610 [Amblyomma americanum].
ACCESSION   XP_077486399
VERSION     XP_077486399.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630273.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..198
                     /product="uncharacterized protein LOC144097610"
                     /calculated_mol_wt=21732
     Region          <2..176
                     /region_name="COG5025"
                     /note="Transcription factor of the Forkhead/HNF3 family
                     [Transcription]"
                     /db_xref="CDD:227358"
     Region          28..102
                     /region_name="Forkhead"
                     /note="Forkhead domain; pfam00250"
                     /db_xref="CDD:459732"
     CDS             1..198
                     /gene="LOC144097610"
                     /coded_by="XM_077630273.1:63..659"
                     /db_xref="GeneID:144097610"
ORIGIN      
        1 mappgplgel lpmgtclrrp sgflvqhian aihksprrml rvshiyealr nkfpfyrfvd
       61 vdaqatwkss vrhalyqnwf ikvpcsrakn sfwslnyser prdwimpeke eqgqplette
      121 altlasdlgl vpsaartppn lesprdirms svptagasql pvvsslsqee sstsnspqtv
      181 gtaaavdagk aevvslef
//
LOCUS       XP_077486406             316 aa            linear   INV 25-NOV-2025
DEFINITION  1-acyl-sn-glycerol-3-phosphate acyltransferase alpha-like
            [Amblyomma americanum].
ACCESSION   XP_077486406
VERSION     XP_077486406.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630280.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..316
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase
                     alpha-like"
                     /calculated_mol_wt=35103
     Region          68..264
                     /region_name="PlsC"
                     /note="1-acyl-sn-glycerol-3-phosphate acyltransferase
                     [Lipid transport and metabolism]; COG0204"
                     /db_xref="CDD:439974"
     CDS             1..316
                     /gene="LOC144097621"
                     /coded_by="XM_077630280.1:76..1026"
                     /db_xref="GeneID:144097621"
ORIGIN      
        1 mragkvvgpw glgagltvlv favlvrksqk frfffrmtty yvlvsavcsl asvpaifrpf
       61 npanmlpisy ilkrlawtvg lkavlqheer iprdkrfilv snhqssldvf gllyvwnelh
      121 pcvpvmksel vytgplaltc ylagsifidr rdreqshqal nnrledvrng ktsfvvfpeg
      181 trnpeptmls fkkgafhmav nsqapvlplv fshyksffcs kerkfnngil tltvlpeist
      241 egltpadvad ltarvrnlmq aeldqqaaqq qlstsadaqp aapggvaala isgsticnrr
      301 cprrrrtrvs rarhsv
//
LOCUS       XP_077486428             346 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097647 [Amblyomma americanum].
ACCESSION   XP_077486428
VERSION     XP_077486428.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630302.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..346
                     /product="uncharacterized protein LOC144097647"
                     /calculated_mol_wt=37735
     CDS             1..346
                     /gene="LOC144097647"
                     /coded_by="XM_077630302.1:121..1161"
                     /db_xref="GeneID:144097647"
ORIGIN      
        1 msypsgqayq afgttssagg qsypfgpqgy qtsgmqqlqq rqlqpqtrqv pqpgfvaips
       61 alqaaalqqq qqqqqqqqqq qklqqqrysv snqgyyppga aaayqqayqa aaalqqqlqq
      121 qqqqqiqqql qqqlqqqqhq lqqqqqqqlq qqqqqqqqqq lqqlqqfarq aaaaaayqgy
      181 paqafavppq tgqtayqqap gypssrsyaa vpttsssayp qnpgtgyttt twssnaaata
      241 dkqfskspwa nyqqaataws nrcpspvnad tsrqnyypfg nnyqstgyps gnsypsgsgy
      301 psansypsgn ggaapsasag yqlqsypqgf pqggtyqvqt ryppar
//
LOCUS       XP_077486435             346 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097647 [Amblyomma americanum].
ACCESSION   XP_077486435
VERSION     XP_077486435.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630309.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..346
                     /product="uncharacterized protein LOC144097647"
                     /calculated_mol_wt=37735
     CDS             1..346
                     /gene="LOC144097647"
                     /coded_by="XM_077630309.1:145..1185"
                     /db_xref="GeneID:144097647"
ORIGIN      
        1 msypsgqayq afgttssagg qsypfgpqgy qtsgmqqlqq rqlqpqtrqv pqpgfvaips
       61 alqaaalqqq qqqqqqqqqq qklqqqrysv snqgyyppga aaayqqayqa aaalqqqlqq
      121 qqqqqiqqql qqqlqqqqhq lqqqqqqqlq qqqqqqqqqq lqqlqqfarq aaaaaayqgy
      181 paqafavppq tgqtayqqap gypssrsyaa vpttsssayp qnpgtgyttt twssnaaata
      241 dkqfskspwa nyqqaataws nrcpspvnad tsrqnyypfg nnyqstgyps gnsypsgsgy
      301 psansypsgn ggaapsasag yqlqsypqgf pqggtyqvqt ryppar
//
LOCUS       XP_077486441             346 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097647 [Amblyomma americanum].
ACCESSION   XP_077486441
VERSION     XP_077486441.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630315.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..346
                     /product="uncharacterized protein LOC144097647"
                     /calculated_mol_wt=37735
     CDS             1..346
                     /gene="LOC144097647"
                     /coded_by="XM_077630315.1:154..1194"
                     /db_xref="GeneID:144097647"
ORIGIN      
        1 msypsgqayq afgttssagg qsypfgpqgy qtsgmqqlqq rqlqpqtrqv pqpgfvaips
       61 alqaaalqqq qqqqqqqqqq qklqqqrysv snqgyyppga aaayqqayqa aaalqqqlqq
      121 qqqqqiqqql qqqlqqqqhq lqqqqqqqlq qqqqqqqqqq lqqlqqfarq aaaaaayqgy
      181 paqafavppq tgqtayqqap gypssrsyaa vpttsssayp qnpgtgyttt twssnaaata
      241 dkqfskspwa nyqqaataws nrcpspvnad tsrqnyypfg nnyqstgyps gnsypsgsgy
      301 psansypsgn ggaapsasag yqlqsypqgf pqggtyqvqt ryppar
//
LOCUS       XP_077486451              98 aa            linear   INV 25-NOV-2025
DEFINITION  annexin A2-like [Amblyomma americanum].
ACCESSION   XP_077486451
VERSION     XP_077486451.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630325.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..98
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..98
                     /product="annexin A2-like"
                     /calculated_mol_wt=10874
     Region          29..94
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     CDS             1..98
                     /gene="LOC144097672"
                     /coded_by="XM_077630325.1:527..823"
                     /db_xref="GeneID:144097672"
ORIGIN      
        1 meaiksemsg dlkngflaiv kcvysppfyf aetlyhamkg lgtndrvlkr vvvsrcekdl
       61 elikqeyldk ykvslasaiq sdtsghyrka llaivqgn
//
LOCUS       XP_077486462             568 aa            linear   INV 25-NOV-2025
DEFINITION  annexin A4-like [Amblyomma americanum].
ACCESSION   XP_077486462
VERSION     XP_077486462.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630336.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..568
                     /product="annexin A4-like"
                     /calculated_mol_wt=58985
     Region          269..333
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          340..405
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          424..489
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          499..564
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     CDS             1..568
                     /gene="LOC144097683"
                     /coded_by="XM_077630336.1:136..1842"
                     /db_xref="GeneID:144097683"
ORIGIN      
        1 msypgggppq ggyppaggyp pppgggygga pggyphppgg pgappggppg gpgsgyppap
       61 ggygpppgga ppyppahgga pppggasygp pggsmplpqp fgagpgsstg ygssgppppy
      121 gappappsgy gppppyggps aapsssygsq psygpasssy gpssgqpgas gygssqysgp
      181 htgaatssty pppppppqgg yggqgsyggg pgggygtgqd ygygagggsr rnlssssyss
      241 gsgtsmggny rprcegtlrp ypnfdaksda qalrtamkgw gtdedaiini lctrtspqrm
      301 eivtafkqmf grdlikdiks elrsnlesvm vgllypmhey larelraamk glgtdedclv
      361 eilctrsndd irrikesykd vfgrdleddi rgdtsghfrr llvsmcnasr degvapdptr
      421 arndahhlqq agvqrwgtde svfqqilasq syeqlllvfr eyngltnhti ieaitremsg
      481 dlrkgflaiv kcvynipfyf aeklyrgmkg mgtsdkavir iivsrcevdm vqikeefsrt
      541 yktsldnfiq sdtsgdyrka llalihgn
//
LOCUS       XP_077486468             395 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077486468
VERSION     XP_077486468.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630342.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..395
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=44018
     Region          7..368
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..395
                     /gene="LOC144097694"
                     /coded_by="XM_077630342.1:484..1671"
                     /db_xref="GeneID:144097694"
ORIGIN      
        1 mrlsskpwfq limngvlsva tfffmsgfal sfskaisqpt lrafveavar ryirltcpvm
       61 vvvlaafllp lladgpadte llaeqlngcy tswwtllvha ndfmplrqmc lnhlwyvaad
      121 mqifvavalp laillkrhrk aafvlaflva tsfaslaaaq tyvwhlfhgi tfgnndirrf
      181 sntleyihfk pfvhvptyac gtvcgflaan vkekrlsqgt kaflwilsag laclvmfvti
      241 pwndgrfpad svsalyggfh rlvwalslww pffacatgeg gfcyrlltwk aflplsrlty
      301 giylthvliq llrmantktp invdeflqlr nsigtfglsi alsyvlyiic egptqqmeri
      361 lfrkrppsrv sgaaaeseta akppceieas tgapk
//
LOCUS       XP_077486477             269 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077486477
VERSION     XP_077486477.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630351.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..269
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=29905
     CDS             1..269
                     /gene="LOC144097704"
                     /coded_by="XM_077630351.1:411..1220"
                     /db_xref="GeneID:144097704"
ORIGIN      
        1 mqvfaitfpf amllsrfpka tvtvlvlasf acstftalrt yfsnllfgmt tgtndgrrvf
       61 ktleliyfrp ithvatyvsg ilagyaavky skariprvvq iilwavstga awfvlfvtlp
      121 wnrenipgpi tnaiyggyhr llwslslcwp ayacatgygg ilgrisswkg flplarltyg
      181 vyllhgvfll lrmglvktrf nlddffqltn tlgiitlsyy fayllflace gpianifecv
      241 ldhkdtktsq satapsttla anqqirrlp
//
LOCUS       XP_077486484             195 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077486484
VERSION     XP_077486484.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630358.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..195
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..195
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=22083
     Region          <78..163
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; cl46765"
                     /db_xref="CDD:481105"
     CDS             1..195
                     /gene="LOC144097714"
                     /coded_by="XM_077630358.1:1..588"
                     /db_xref="GeneID:144097714"
ORIGIN      
        1 mlcgyltvry rkanipkllq gsawvaslal sllvifvptp wnrghmpaki itavyagvhr
       61 viwtlpfwwl hyacitgrgg flnallstrv frvlgrlvys tylihfplll vrigiiktpl
      121 qaeeffqtld figisvlsmt lalllnvtce tpieqldrlv frsvdkrimn dlnsidakkq
      181 plpvviennh eksrl
//
LOCUS       XP_077486501             332 aa            linear   INV 25-NOV-2025
DEFINITION  DNA-dependent metalloprotease SPRTN-like [Amblyomma americanum].
ACCESSION   XP_077486501
VERSION     XP_077486501.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630375.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..332
                     /product="DNA-dependent metalloprotease SPRTN-like"
                     /calculated_mol_wt=37905
     Region          69..235
                     /region_name="SprT"
                     /note="SprT homologues; smart00731"
                     /db_xref="CDD:214794"
     CDS             1..332
                     /gene="LOC144097733"
                     /coded_by="XM_077630375.1:195..1193"
                     /db_xref="GeneID:144097733"
ORIGIN      
        1 msmtsadeet llspelalgm teeyeyhesl pklnessiek pagtsvvshs srkdlsvvde
       61 fwelhdpnpd vhrlfdefne ayfygklnev evrwsrrmvl csglceykvn gglctvklse
      121 pllkfrprkd lvetllhemi hayllvtkgq rahgpefheh mdrinkasga nitvfhhfhk
      181 etdyyrthww rcngpckhlm pffgvikrvm nrppseknvw waehqrkcgg tfikirepdp
      241 ksskrkqkdg tlpaknaakk pklmdgadig tlptlptqpt lkdflgqktq paeqtsrkss
      301 aaevkfirdi lcsdsesddd cvitaaymtv ke
//
LOCUS       XP_077486561             257 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097803 [Amblyomma americanum].
ACCESSION   XP_077486561
VERSION     XP_077486561.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630435.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..257
                     /product="uncharacterized protein LOC144097803"
                     /calculated_mol_wt=28631
     Region          21..109
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..257
                     /gene="LOC144097803"
                     /coded_by="XM_077630435.1:25..798"
                     /db_xref="GeneID:144097803"
ORIGIN      
        1 maatmsveps amaseassqk rvnwptvtte aliriwednl sslrsntrna riynamaell
       61 naglpsgegp ytakqvrqkl enlnkqyrkl rrsgsstgsk gvqwpfywql hsflgtlpvn
      121 dsdlmqesve mpedpahtgq eivfeyednl ipvpatipct leegndstag sepagreqgt
      181 sgercgksnl rrkrpsssll relmamhqqa eeraakaake sselrkqlie lqkesnevqk
      241 gmlqvmkdyl qskenke
//
LOCUS       XP_077486626             112 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097891 [Amblyomma americanum].
ACCESSION   XP_077486626
VERSION     XP_077486626.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630500.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..112
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..112
                     /product="uncharacterized protein LOC144097891"
                     /calculated_mol_wt=11352
     CDS             1..112
                     /gene="LOC144097891"
                     /coded_by="XM_077630500.1:36..374"
                     /db_xref="GeneID:144097891"
ORIGIN      
        1 mffsaaapsd pggfevgagr lralspalrf hgdggllics ypsdrggfea gtdclratsp
       61 avrfhggggf flsssphsgh ggfevgagrl ramtpalrfh gdgglfvrsc pl
//
LOCUS       XP_077486631             112 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097891 [Amblyomma americanum].
ACCESSION   XP_077486631
VERSION     XP_077486631.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630505.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..112
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..112
                     /product="uncharacterized protein LOC144097891"
                     /calculated_mol_wt=11352
     CDS             1..112
                     /gene="LOC144097891"
                     /coded_by="XM_077630505.1:36..374"
                     /db_xref="GeneID:144097891"
ORIGIN      
        1 mffsaaapsd pggfevgagr lralspalrf hgdggllics ypsdrggfea gtdclratsp
       61 avrfhggggf flsssphsgh ggfevgagrl ramtpalrfh gdgglfvrsc pl
//
LOCUS       XP_077486677             321 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097958 [Amblyomma americanum].
ACCESSION   XP_077486677
VERSION     XP_077486677.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630551.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..321
                     /product="uncharacterized protein LOC144097958"
                     /calculated_mol_wt=35164
     CDS             1..321
                     /gene="LOC144097958"
                     /coded_by="XM_077630551.1:52..1017"
                     /db_xref="GeneID:144097958"
ORIGIN      
        1 mpkrkckfte qlgkeypfik ktkseadvrc gacgadfsva hggrsdivkh issvkhkqsv
       61 saaasssavt rffrqerage kelqlaaseg ifafhtiahs hsfrsmdcts klvqklfsek
      121 fscartkaea ialnvlapsa eeelktdlqe akfvsvfcda snhkdlkvlp imvryftaaa
      181 gvqtkllein tlpgetsamv sqyimdalea nqvtsklvaf sadnancnfg gaarngknnv
      241 fsrlqdsvdh kilgigcaah ivhntvqtaa dclpldieav ivkiysyfyi ytvwnrsksf
      301 vilhqwstsg fwaiarpggw h
//
LOCUS       XP_077486686             488 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097968 [Amblyomma americanum].
ACCESSION   XP_077486686
VERSION     XP_077486686.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630560.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..488
                     /product="uncharacterized protein LOC144097968"
                     /calculated_mol_wt=54152
     Region          269..298
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          299..321
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          322..358
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          372..401
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          402..428
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     CDS             1..488
                     /gene="LOC144097968"
                     /coded_by="XM_077630560.1:128..1594"
                     /db_xref="GeneID:144097968"
ORIGIN      
        1 merveefgdp wflliaedyi vlkletpwpv dvplpdgysm dgddghllvt nqaslvsatn
       61 slretrisea lfagcaafes snvfeasare vlrfagkici vhnrdkiyrl irifpnakvl
      121 alphdlcfha vdrdelyidr weqlvptsel relegstels cassllftee tvleivrtcp
      181 nvrridvasa lgaflqleks sppgwrhnas kfthlmlgfa akisnsdhvl taraddvkla
      241 aekfpllevl kvivgslpef aaistfhfir clsvslaasi rfadvdaelk sllktwprle
      301 slslsccggl rlaslamlcp elktlrlvsc emssddtpld ydafpllrtv elgvklpkvv
      361 fdaffyatsg tlrnlrlagd ascrgflhlc clhdepisfp cleelaltvd alqlepedlh
      421 hlltslpalr hlstdsydlr lfvenycvpr gsvslswcec vfcavhrpcd gnsdeeadvi
      481 rdclglpm
//
LOCUS       XP_077486687             146 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097976 [Amblyomma americanum].
ACCESSION   XP_077486687
VERSION     XP_077486687.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630561.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..146
                     /product="uncharacterized protein LOC144097976"
                     /calculated_mol_wt=15786
     CDS             1..146
                     /gene="LOC144097976"
                     /coded_by="XM_077630561.1:275..715"
                     /db_xref="GeneID:144097976"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrppla altsvlccqq pasigpcvlp afgcvippdp
       61 fmlktkscme vvqatklrvi fardleqagk lvdgqwysls kasffsktkt iflgswamyl
      121 gtcgqdnagr hasasskkkh etvnky
//
LOCUS       XP_077486702             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144097999 [Amblyomma americanum].
ACCESSION   XP_077486702
VERSION     XP_077486702.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630576.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144097999"
                     /calculated_mol_wt=14022
     CDS             1..127
                     /gene="LOC144097999"
                     /coded_by="XM_077630576.1:134..517"
                     /db_xref="GeneID:144097999"
ORIGIN      
        1 mapgkkrpvr ewnrlaywcp dhqrledmpi mqfikkeimt spepqstyaa eqrkcaqqrp
       61 sgsktnagta attgsgsdlr idtpattgsg sdlsidtqat trnkkkrsae kenepespkr
      121 apkcfrv
//
LOCUS       XP_077486717             131 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098017 [Amblyomma americanum].
ACCESSION   XP_077486717
VERSION     XP_077486717.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630591.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..131
                     /product="uncharacterized protein LOC144098017"
                     /calculated_mol_wt=14249
     CDS             1..131
                     /gene="LOC144098017"
                     /coded_by="XM_077630591.1:81..476"
                     /db_xref="GeneID:144098017"
ORIGIN      
        1 metgsdavmv atslekladi eevladkdqr alqirklali ggettdeatk limktlvktp
       61 valrytlegr gekqsfknlr lysaivaavq rriptatieg innrvaaflr gardrdggrr
      121 armkgvdpsp q
//
LOCUS       XP_077486722             128 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098028 [Amblyomma americanum].
ACCESSION   XP_077486722
VERSION     XP_077486722.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630596.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..128
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..128
                     /product="uncharacterized protein LOC144098028"
                     /calculated_mol_wt=14174
     CDS             1..128
                     /gene="LOC144098028"
                     /coded_by="XM_077630596.1:100..486"
                     /db_xref="GeneID:144098028"
ORIGIN      
        1 mapgkkrpvr ewnrlaywcp ghqrledmpi mqfikkeimt spepqstyaa eqrkcaqqrp
       61 sgsktnagta attgsssdlr idtpattgsg sdlrsidtqa tirnnkkrsa ekenepelpk
      121 rapkcfrv
//
LOCUS       XP_077486731             477 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098041 [Amblyomma americanum].
ACCESSION   XP_077486731
VERSION     XP_077486731.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630605.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..477
                     /product="uncharacterized protein LOC144098041"
                     /calculated_mol_wt=53462
     Region          45..>274
                     /region_name="SWIM"
                     /note="Uncharacterized protein, contains SWIM-type Zn
                     finger domain [Function unknown]; COG4715"
                     /db_xref="CDD:443750"
     Region          274..464
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(338,376,390,392)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..477
                     /gene="LOC144098041"
                     /coded_by="XM_077630605.1:6..1439"
                     /db_xref="GeneID:144098041"
ORIGIN      
        1 mscglrshns qgwtsktqsl npffgkehiv ryieskgark hresglrlft sghlqklvfs
       61 tedtaetvvk aqvlasmatr taynvgvkll kcdgevisgn csciagkggv ckhvccvlyg
      121 lmhiaqhdlt nvpdavacte gerqwynprd pkkvteqfqh ivfskdtter lsdaprhhkr
      181 ravysslrse dtnlstshli nlhgklrask ldcfadvlea ndfmpakrkk aessdpedvp
      241 glparwlera eqrdallpyt qeevaaleaa trlqsgcllw hryregmita slahrvytwv
      301 ktcqtkmgph darsltaavm gvrkvratfa mkrgllcepe arqafqeqnd ghvelevset
      361 glflsrkhsf lgaspdglvk ckcceprlle ikvpvkvedf aktqfrdgql kksskyytqv
      421 qvqmgvtgld fcvlfvyske kcvqilvpfd sdffaevakr cnffatmyll phissnc
//
LOCUS       XP_077486738             136 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098047 [Amblyomma americanum].
ACCESSION   XP_077486738
VERSION     XP_077486738.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630612.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..136
                     /product="uncharacterized protein LOC144098047"
                     /calculated_mol_wt=14478
     CDS             1..136
                     /gene="LOC144098047"
                     /coded_by="XM_077630612.1:99..509"
                     /db_xref="GeneID:144098047"
ORIGIN      
        1 mssdrtvhha amdadrtvvd tpqdgqqppe istfsalfln ipdqlfptqa avvvidghtt
       61 rqgsearpss cirdprqqev egvdpvessy tasavpepdy mphppaseat aqsartrped
      121 ssvrnltpaa aridsa
//
LOCUS       XP_077486750             232 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098063 [Amblyomma americanum].
ACCESSION   XP_077486750
VERSION     XP_077486750.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630624.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..232
                     /product="uncharacterized protein LOC144098063"
                     /calculated_mol_wt=26206
     Region          38..>108
                     /region_name="SWIM"
                     /note="Uncharacterized protein, contains SWIM-type Zn
                     finger domain [Function unknown]; COG4715"
                     /db_xref="CDD:443750"
     CDS             1..232
                     /gene="LOC144098063"
                     /coded_by="XM_077630624.1:79..777"
                     /db_xref="GeneID:144098063"
ORIGIN      
        1 mirlqcgfkk icqldsffrk anlikgekll nhvysveqii ennearlkgk cvsqvsdsvv
       61 ydvclelaws chekqellvr egrctckagi aakckhaaav clyvdqhevk sctsepqkwg
      121 kpkgnpkrsl elflfphgmk nvafeslark hmpyaqnevt kdsrkllias arrhrtrvlq
      181 cimasklkpq hqkickessa svlcrqdslc frsshgcava qmdwqqlavk lc
//
LOCUS       XP_077486755             108 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098073 [Amblyomma americanum].
ACCESSION   XP_077486755
VERSION     XP_077486755.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630629.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..108
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..108
                     /product="uncharacterized protein LOC144098073"
                     /calculated_mol_wt=11913
     CDS             1..108
                     /gene="LOC144098073"
                     /coded_by="XM_077630629.1:133..459"
                     /db_xref="GeneID:144098073"
ORIGIN      
        1 mapgkkrpvr ewnrlaywcp ghqrledmpi mqfikkeimt spepqstyaa eqrrcaqqrp
       61 sgsktnagta attgsgsdlh idtpatnrsg sdlrintgdh qeqqetfg
//
LOCUS       XP_077486775             142 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077486775
VERSION     XP_077486775.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630649.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..142
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=15776
     Region          <7..114
                     /region_name="Piwi-like"
                     /note="PIWI domain. Domain found in proteins involved in
                     RNA silencing. RNA silencing refers to a group of related
                     gene-silencing mechanisms mediated by short RNA molecules,
                     including siRNAs, miRNAs, and heterochromatin-related
                     guide RNAs. The central component...; cl00628"
                     /db_xref="CDD:412485"
     CDS             1..142
                     /gene="LOC144098097"
                     /coded_by="XM_077630649.1:12..440"
                     /db_xref="GeneID:144098097"
ORIGIN      
        1 mddsastsrl evikdlkvmm kdmlkafyha tkhkperiif frdgvsegqf mdvrnhegts
       61 gpshyyivwd gssftsdelq klcyylyhty arcshsvsip apvyyahlaa yrsrnqvksk
      121 advssssgds sggsadsvsi sq
//
LOCUS       XP_077486782             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098107 [Amblyomma americanum].
ACCESSION   XP_077486782
VERSION     XP_077486782.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630656.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144098107"
                     /calculated_mol_wt=14774
     CDS             1..133
                     /gene="LOC144098107"
                     /coded_by="XM_077630656.1:105..506"
                     /db_xref="GeneID:144098107"
ORIGIN      
        1 mqesvemped pahtgqeivf eyednlipvp atipctleeg ndstagsepa greqgtsger
       61 cgksnlrrkr psssllrelm amhqqaeera akaakessel rkqlielqke snevqkgmlq
      121 vmkdylqske nke
//
LOCUS       XP_077486797             101 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098136 [Amblyomma americanum].
ACCESSION   XP_077486797
VERSION     XP_077486797.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630671.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..101
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..101
                     /product="uncharacterized protein LOC144098136"
                     /calculated_mol_wt=11035
     Region          74..>99
                     /region_name="PDDEXK_nuclease-like"
                     /note="PDDEXK family nucleases; cl40440"
                     /db_xref="CDD:477358"
     CDS             1..101
                     /gene="LOC144098136"
                     /coded_by="XM_077630671.1:792..1097"
                     /db_xref="GeneID:144098136"
ORIGIN      
        1 mplvphgfqv hvapeivctn agtaemptpp rlfqscrkwh lqdgdfsaae aailkglqvs
       61 sqeaidlekn trqqsgsemw erarvyrlta ssfgvvvkkp t
//
LOCUS       XP_077486803             190 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098146 [Amblyomma americanum].
ACCESSION   XP_077486803
VERSION     XP_077486803.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630677.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..190
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..190
                     /product="uncharacterized protein LOC144098146"
                     /calculated_mol_wt=21882
     CDS             1..190
                     /gene="LOC144098146"
                     /coded_by="XM_077630677.1:314..886"
                     /db_xref="GeneID:144098146"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedrrr rhlnrrltat mkrlsgvhvl
       61 nhehrfldas gepmlslfaa drgidqmsdy rrgprqdlrt rdsvgfkgkt rrgvrrapvs
      121 svrsqraqdg pllgllltap prrcrsrlkc sckrpflgpp hcfladrerp vscpyslsve
      181 idarhqnrav
//
LOCUS       XP_077486814             125 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098164 [Amblyomma americanum].
ACCESSION   XP_077486814
VERSION     XP_077486814.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630688.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..125
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..125
                     /product="uncharacterized protein LOC144098164"
                     /calculated_mol_wt=13474
     CDS             1..125
                     /gene="LOC144098164"
                     /coded_by="XM_077630688.1:282..659"
                     /db_xref="GeneID:144098164"
ORIGIN      
        1 minsektecq rltsfksaat tttltrhskp gwwwrrpqll peppfyatsw rasgselgnd
       61 aqksaqseae aqvrllvsis ciksedlrrv lgvsilgnsf apapsattpi rsgaqrksvs
      121 asldg
//
LOCUS       XP_077486824             168 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098180 [Amblyomma americanum].
ACCESSION   XP_077486824
VERSION     XP_077486824.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630698.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..168
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..168
                     /product="uncharacterized protein LOC144098180"
                     /calculated_mol_wt=18904
     Region          5..85
                     /region_name="THAP"
                     /note="The THAP domain is a putative DNA-binding domain
                     (DBD) and probably also binds a zinc ion; smart00980"
                     /db_xref="CDD:214951"
     CDS             1..168
                     /gene="LOC144098180"
                     /coded_by="XM_077630698.1:63..569"
                     /db_xref="GeneID:144098180"
ORIGIN      
        1 mpttcvaygc naryvkgskl gffrfpnasr drlrreawik avrrlddrgr hwapssasrl
       61 cgnhfetgrp rmsprhrdfv ptlfsfsckk akrasavsrf qramertkkk klreqgstps
      121 gtlvsavqgg gdddmvidgd segdlhgtqs qqssdlccsw rffkqrpk
//
LOCUS       XP_077486841             184 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098215 [Amblyomma americanum].
ACCESSION   XP_077486841
VERSION     XP_077486841.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630715.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..184
                     /product="uncharacterized protein LOC144098215"
                     /calculated_mol_wt=20061
     CDS             1..184
                     /gene="LOC144098215"
                     /coded_by="XM_077630715.1:84..638"
                     /db_xref="GeneID:144098215"
ORIGIN      
        1 mspatelvga vadhmatrlp npfdsdgahv geavlslppa alleamvngn etsqddvpaq
       61 spllnsrvin edelpvqeec lnasrrtvvv pnqsssegip ptqtartrag gprvaavert
      121 lapelaarit aieaeerrke elhqldvqlr rsqlaeqrlk vkmqrkllsl diqirkkqle
      181 amqr
//
LOCUS       XP_077486865             372 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098245 [Amblyomma americanum].
ACCESSION   XP_077486865
VERSION     XP_077486865.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630739.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..372
                     /product="uncharacterized protein LOC144098245"
                     /calculated_mol_wt=38420
     Region          <43..330
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     CDS             1..372
                     /gene="LOC144098245"
                     /coded_by="XM_077630739.1:696..1814"
                     /db_xref="GeneID:144098245"
ORIGIN      
        1 mskdgatrgr tnghkqerlt qrshlarvpt agnhgpsgrs qaapsalpln gtaqrsfaqv
       61 vqervvtaap pqlrsrtanv tthgegttsn gartvaptkg sptnlplpta apppvlrsrt
      121 anvttrgegt tshgartvap tkgsptnlps ptaapppklr srrtanvttr gegtishgar
      181 tvaptkgspt nlpsptaapp pqlrsrrtan vttrgegtts nrartvaptk gsptnllspt
      241 aapppkrrsr tanvttrgeg ttsngartva ptkgsptnlp sptaapppqr rsrrtanvtt
      301 rgegttsnra rtvaptkgsp tnlpaptaap veavqarngv gnaklqndel kiqvfssrad
      361 wshsfsffvc vq
//
LOCUS       XP_077486910             315 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098272 [Amblyomma americanum].
ACCESSION   XP_077486910
VERSION     XP_077486910.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630784.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..315
                     /product="uncharacterized protein LOC144098272"
                     /calculated_mol_wt=35348
     Region          8..83
                     /region_name="Myb_DNA-bind_5"
                     /note="Myb/SANT-like DNA-binding domain; pfam13873"
                     /db_xref="CDD:433544"
     CDS             1..315
                     /gene="LOC144098272"
                     /coded_by="XM_077630784.1:354..1301"
                     /db_xref="GeneID:144098272"
ORIGIN      
        1 megkkrvnfs eeersvlidl vskyqtiven krtdgvsldk kkktwkeitd vyncrpnvrf
       61 rteaqlrkcw dnlkekwrkg kaddmkevft tgggsapatq ltdelervga aashmstrve
      121 npfdsdrgrh avtplskstp svvsllqpmq veslepsnds iyqwdnpasp nnessqvaci
      181 qelqneataa evelsaerep adlqpavpql asplqaaplq aaplqetaqh papaqrrmlt
      241 kraaveeels arlcsvdedr krkrrehllk mklmraehal hmkhskqlhe lecatkkakl
      301 ellmlkiaav kkask
//
LOCUS       XP_077486920             225 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098280 [Amblyomma americanum].
ACCESSION   XP_077486920
VERSION     XP_077486920.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630794.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..225
                     /product="uncharacterized protein LOC144098280"
                     /calculated_mol_wt=24224
     CDS             1..225
                     /gene="LOC144098280"
                     /coded_by="XM_077630794.1:66..743"
                     /db_xref="GeneID:144098280"
ORIGIN      
        1 meespldwwk kseqalppll ppqprmrpla cllpallaiv agfaasvrcs rrqlvayavl
       61 atpfaflhav lpvlcfdqgt mtseharaga ggpqspihld kgasvgfvca satwvrrqqv
      121 eaatklgryl deqaamrsrk lpvlglfqmy pkyipsflht vafgecgspv rinccglall
      181 vsiviiakrw ssasmaaqlq aqldaqpvaa qvahgnrppm drtrg
//
LOCUS       XP_077486960             967 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-4-like [Amblyomma americanum].
ACCESSION   XP_077486960
VERSION     XP_077486960.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630834.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..967
                     /product="protein argonaute-4-like"
                     /calculated_mol_wt=108980
     Region          202..271
                     /region_name="ArgoN"
                     /note="N-terminal domain of argonaute; pfam16486"
                     /db_xref="CDD:465134"
     Region          281..332
                     /region_name="ArgoL1"
                     /note="Argonaute linker 1 domain; pfam08699"
                     /db_xref="CDD:462567"
     Region          338..468
                     /region_name="PAZ"
                     /note="PAZ domain; pfam02170"
                     /db_xref="CDD:460472"
     Region          495..924
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(627,631,643..646,649,658,661,665,669)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(696,698,774,914)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..967
                     /gene="LOC144098300"
                     /coded_by="XM_077630834.1:27..2930"
                     /db_xref="GeneID:144098300"
ORIGIN      
        1 mesaprsqrr scgtgrptgr spgapsalsf aqavqqrvvr aappqilsst aagtavitra
       61 egtngngaht vaptegvptt stsptaapvq arngvgntks qndevsiqei ertlpshfpr
      121 rptqgklgrp iqltanhfsv eipsgnvfhy dveicsesrk etnkvpekrk yrcistkinr
      181 lviellvkky rvdlsgcmpa fdgrknlytr relnfrertf tvdfeedqri qkfivkiqya
      241 atvnldalqa vfdrrvshvp qevlqavdiv lrhgpsiklt pvgrsffrpp apgeyntlgg
      301 grevwfgyyt svrpaqwkpm lnvdisataf yeplpvtdfm cklfsdcrre msagdlknlr
      361 dfqnvrlnke lkglrvkvth lsyprkykvl rvtkepakkl yfkmedgtrc svadyfqnry
      421 drlkypnlpc iqtgsenhpv ylplevcviv egqhckkkld enqtsemikr taqppakrfn
      481 eirqsvrdlv sssdqclref gikisteptq lkgrvldpps lvfennyigk pregtwelrg
      541 rhfyksatlt rwtllnlsqf aqrdsldnfv kmlirvgqel gmsieqpldv tttdtnrkpv
      601 wnilleeqrk tanlemviiv lakntsyadi kqvaeteigl rtqcvmdnnv ikkcnaalit
      661 nlcqkmnakl gginnsllpk ekpkifqkpv iiigadvthp apgdklrpsi aacvgsldai
      721 pykfhasvrv qmadsaatsr leiikdlkdm mkdtlkafyh atrhkperii fyrdgvsegq
      781 fmevrnrevs airlacqels pnetyepalt fivvqkrhht rfmpandrdg vgkcrnraar
      841 ttvdsvvthp ldfdfylcsh fgiqgtsrps hyyilwddss ftadevqyls yhlchtyarc
      901 trsvsipapv yyahlaayra kkhlmskvdv sssssdssgg sadavstrqy veavkvlesl
      961 ktgmyfv
//
LOCUS       XP_077486974             249 aa            linear   INV 25-NOV-2025
DEFINITION  sodium-dependent multivitamin transporter-like [Amblyomma
            americanum].
ACCESSION   XP_077486974
VERSION     XP_077486974.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630848.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..249
                     /product="sodium-dependent multivitamin transporter-like"
                     /calculated_mol_wt=26251
     Region          <1..187
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     CDS             1..249
                     /gene="LOC144098303"
                     /coded_by="XM_077630848.1:66..815"
                     /db_xref="GeneID:144098303"
ORIGIN      
        1 mrgvflagvv gasmstvssi inshsavfyv dvvtpyfska kkrpsyvisi iaiasgvmmt
       61 gfatllpymg taarvtmvic nsisgplcgl fllallfpws ntkgaasstl vslalllwhs
      121 vgrsfsgiep prmdvsidgc lanvtavsts alrhvrldvf pfyrisanwm svfsvlftia
      181 tglvvslatg grrevdkslq lsskqvlcvw aqvgllsrkt lvsdgspems tskeaislip
      241 rsgeeagac
//
LOCUS       XP_077487005             134 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098324 [Amblyomma americanum].
ACCESSION   XP_077487005
VERSION     XP_077487005.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630879.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..134
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..134
                     /product="uncharacterized protein LOC144098324"
                     /calculated_mol_wt=14311
     CDS             1..134
                     /gene="LOC144098324"
                     /coded_by="XM_077630879.1:141..545"
                     /db_xref="GeneID:144098324"
ORIGIN      
        1 myadledtid vssqrvattp vhkvsrrlrg lqpefgqltt laramtstta sqttqhsgna
       61 plfvlhasrs ppsfhalrpg gvmqriqrmp qhdhgscrrg tqrrnsgggs ddacggvegs
      121 ygksslprtp ergs
//
LOCUS       XP_077487037             213 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098342 [Amblyomma americanum].
ACCESSION   XP_077487037
VERSION     XP_077487037.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630911.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..213
                     /product="uncharacterized protein LOC144098342"
                     /calculated_mol_wt=23267
     Region          147..209
                     /region_name="bHLH_TS_amos_like"
                     /note="basic helix-loop-helix (bHLH) domain found in
                     Drosophila melanogaster protein Amos and similar proteins;
                     cd19715"
                     /db_xref="CDD:381558"
     Site            order(155,159,161..163,165,170,188..191)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381558"
     Site            order(168..169,172..173,175..176,192,195,199,201..202,206,
                     208..209)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:381558"
     CDS             1..213
                     /gene="LOC144098342"
                     /coded_by="XM_077630911.1:66..707"
                     /db_xref="GeneID:144098342"
ORIGIN      
        1 mtmepitmts qlvtttysmt kdvpvfsias pgqpafypan hhhsrnphqa vsdqnqpagi
       61 dedsdlllfa danrffddet krllalrgrl rgfdndetll ysdntlgsat gdgksavgrl
      121 glkrkqsvgs aevaspassp ppttgqapvv vkkrrlaana rerrrmhgln vafdrlrqvv
      181 psigddrkls kfetlqmaqs yitaltellv rdc
//
LOCUS       XP_077487050             215 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098350 [Amblyomma americanum].
ACCESSION   XP_077487050
VERSION     XP_077487050.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630924.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..215
                     /product="uncharacterized protein LOC144098350"
                     /calculated_mol_wt=23387
     Region          150..212
                     /region_name="bHLH_TS_amos_like"
                     /note="basic helix-loop-helix (bHLH) domain found in
                     Drosophila melanogaster protein Amos and similar proteins;
                     cd19715"
                     /db_xref="CDD:381558"
     Site            order(158,162,164..166,168,173,191..194)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381558"
     Site            order(171..172,175..176,178..179,195,198,202,204..205,209,
                     211..212)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:381558"
     CDS             1..215
                     /gene="LOC144098350"
                     /coded_by="XM_077630924.1:204..851"
                     /db_xref="GeneID:144098350"
ORIGIN      
        1 mnttsaalam avncltdval leqqshhdys cysthqhhqh eqgkplaygi prsdgcptpv
       61 sspatlppcs paaespaslt elcpiprpga vghldsmlpf raptreeges defdsdaeng
      121 sefssedgkh tsfgkgtgsk rkqsdkapps pvvlrkrrla anarerrrmh slnvafdrlr
      181 evvpslgndr klskfetlqm aqsyinalse lllrq
//
LOCUS       XP_077487072             433 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098360 [Amblyomma americanum].
ACCESSION   XP_077487072
VERSION     XP_077487072.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630946.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..433
                     /product="uncharacterized protein LOC144098360"
                     /calculated_mol_wt=48006
     Region          <8..>107
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     Region          <175..>278
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     CDS             1..433
                     /gene="LOC144098360"
                     /coded_by="XM_077630946.1:76..1377"
                     /db_xref="GeneID:144098360"
ORIGIN      
        1 myqstsware lfaalgvnts iknlrigfrg anatcgdlla salrkntsll eldlradvdd
       61 gfmicvadsl sqnaaldrlv ftnatvyarg vlalchalrt nktlqqlmfp tfqasiterv
      121 llanilahgd gyrrvrlpft apdmsrlstr lacpasspev lihvdidrvp nvqfkllcda
      181 lssssrvhtc sfhieedpae ktamlsemlr vnrsirslei vmkrergkfi rkvlrslran
      241 egvcdmtits vsmgmetava lsnffarnrt ltkfqlsflg gnfsqfvdll yhgmstnpvi
      301 vdveiaaayt ygrthqline tvrrnrhaln ravdfvvlhs mdkayaeafe lfngrpcfls
      361 lltktlgrpe cealvavasa enflrdnylv itgivksvlv chpaddgctq ldelnaqcwh
      421 vivshlkvsd vlp
//
LOCUS       XP_077487107             459 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098382 [Amblyomma americanum].
ACCESSION   XP_077487107
VERSION     XP_077487107.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630981.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..459
                     /product="uncharacterized protein LOC144098382"
                     /calculated_mol_wt=50527
     Region          197..241
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          277..444
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..459
                     /gene="LOC144098382"
                     /coded_by="XM_077630981.1:1..1380"
                     /db_xref="GeneID:144098382"
ORIGIN      
        1 mwllifkhsf lslgkpssdp thpdyvpsif pyrasgnvaq kisrfqraar kdglkeasrp
       61 aacnpgpsvl dcglnttgag qdaddtalsa tatvsvvenc hmetetdlsm qnishlieen
      121 grlkqrlasl eeecsaakqk agelenkhtn haiitkevvm qsaktvkfyt gfvstamfta
      181 flqfvlsawk pscatwldae tqlimvlmrl rlglltqdls crfgisnatv ssifhawldv
      241 lvanmtklii wpsrqtlqan rpaafqdpff dgvtgiidct evfiqrpilm tarsqtcshy
      301 khhntvkilv vispsgaitf vskawggrtp dkeitlrsgl ldkvkegdvf lvdrgfrcge
      361 mfaargatll mssltknraq lpgaevttsr kvssvrihve raiqrlkvfr ifqtvlplnf
      421 vkrtgdknla tidkvvvvcs alvnlqtpii knsqrrsas
//
LOCUS       XP_077487119             722 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098390 [Amblyomma americanum].
ACCESSION   XP_077487119
VERSION     XP_077487119.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077630993.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..722
                     /product="uncharacterized protein LOC144098390"
                     /calculated_mol_wt=80547
     Region          5..89
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..722
                     /gene="LOC144098390"
                     /coded_by="XM_077630993.1:1..2169"
                     /db_xref="GeneID:144098390"
ORIGIN      
        1 mpsfcaafgc antggrddvv fhtfpkdrki sarwvtavkr knfvptkstv lcsdhfrdsd
       61 yhrslgtmra lgisiksarl kpgvvpsvfp hnrstsrppr aalakrkrre vleqllenla
      121 pqeaaaslpp spsealvaes vaeaddvann vpdttdksfq vvvrpptlck glqakvkapk
      181 tsrktqtqpq gvsvgvqvgs flvdaatqvn fmvepyeedd sltdcepepe hhddcspdsd
      241 swhecdncdg hlcsqqqsai gdsergivlv qkkhlkalfe vchtclapct csircegtli
      301 eihawcpsrh qlcwknqdvv rqqpvlnlql caallfsgnn ptassrmlss igvevvtdrt
      361 ffniqkihlw paidrvwrqh qeqllkdvrg kevalagdgr adspgfsakf gtyslldvqn
      421 nkllhfelvq sneaggscym eleglkrglq fleyqgitvi alvtdrhiqi kahmrrerpg
      481 iqhkfdvwhv akgvfkkvta iskrrdckdl qlwaksiqnh lywsaasshg hsdhtvpkwl
      541 sllnhirdvh vhndalfpac ihdkpecrmw lkkeskafek lnniatskvl ladmpklstk
      601 hqtygletfh slllhfapkn cqysylgmka rtqlavmhyn encarsqacr lngaarwnlk
      661 ypkatggkat acpvkeqtty gyaqtllnmv mveasrasea kktalmqtpp plssqfsrvp
      721 ke
//
LOCUS       XP_077487132             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098397 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077487132
VERSION     XP_077487132.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631006.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144098397 isoform X1"
                     /calculated_mol_wt=15029
     CDS             1..141
                     /gene="LOC144098397"
                     /coded_by="XM_077631006.1:141..566"
                     /db_xref="GeneID:144098397"
ORIGIN      
        1 mcqmdhnfff vlrelteife kahsinpayl lgpglvrsvq dsqaepaasp qpgpsrvtat
       61 aspipgssqp stsrgstaag dsqppspsaq psplqqqpgp arkrprtdts ravlesavah
      121 levaeanrqq rhrermaleg r
//
LOCUS       XP_077487140             140 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098397 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077487140
VERSION     XP_077487140.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631014.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..140
                     /product="uncharacterized protein LOC144098397 isoform X2"
                     /calculated_mol_wt=14942
     CDS             1..140
                     /gene="LOC144098397"
                     /coded_by="XM_077631014.1:141..563"
                     /db_xref="GeneID:144098397"
ORIGIN      
        1 mcqmdhnfff vlrelteife kahsinpayl lgpglvrsvq dqaepaaspq pgpsrvtata
       61 spipgssqps tsrgstaagd sqppspsaqp splqqqpgpa rkrprtdtsr avlesavahl
      121 evaeanrqqr hrermalegr
//
LOCUS       XP_077487151             762 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077487151
VERSION     XP_077487151.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631025.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..762
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=84452
     Region          80..206
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          333..706
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..762
                     /gene="LOC144098413"
                     /coded_by="XM_077631025.1:137..2425"
                     /db_xref="GeneID:144098413"
ORIGIN      
        1 masfvrtdsl saalffslvl llftsvppci gddsetsgas aeqpevnyvd klkewianam
       61 dsatkslkrq llsvdlstqc slglvkfvrg irnlepwavr lldatgkfpt galqmsradl
      121 gafdecvetv vldsfghesa rgqycnlqvy agniskleef issaiafthp rikkfsdhik
      181 gpgapflsig icaidacsdq eiqslikavl ppvvdiavsn cvtaippgwt kaqlmiaafl
      241 atlaltiilg syvdvkrsrg ndkscntggl lsflvvfsvv sntrmilqpa addstdsysl
      301 rflhgvryls ltwivigrcy vstsvvwsrm vntiiyaesw enilaglgya aldtffflsg
      361 fllaavvckq krsslcavis lvvkryirtt vpvfflimcl yllplvtsgp daigyftkvh
      421 ndfmwqwpll llqvqnygft mdargpslah lwylsldfqf fllclpilil lknrpklava
      481 tflllsvvgc svaawqvash nmtpflvalt espltflatr syyffyphyh avcyfmgcvt
      541 fffvrryker kvsevlqraa wciaiagglc sicmkgpwyr tkdpttvfgn lctafsdril
      601 wsvflawltf asaterggfv stflswgafa plsrlangvy iihaffiglt vhisrermff
      661 shfvvvsfsf tvlvwsymlg yllfvfceap tdrlgklife prlrkanndr ndealnangp
      721 eeklicaiat lgnseistas ckgksifndf hyassnlasc hl
//
LOCUS       XP_077487164             118 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098422 [Amblyomma americanum].
ACCESSION   XP_077487164
VERSION     XP_077487164.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631038.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..118
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..118
                     /product="uncharacterized protein LOC144098422"
                     /calculated_mol_wt=13431
     CDS             1..118
                     /gene="LOC144098422"
                     /coded_by="XM_077631038.1:113..469"
                     /db_xref="GeneID:144098422"
ORIGIN      
        1 mkdlvlllhe hwasvvlvfk ilqrhcddph karfedrrrr qlnrrllatl krlsgmhiln
       61 pehrfldasg kpilslfaad ryhvardrgi npsaplflcs gksrvkvaai ilplqsal
//
LOCUS       XP_077487178             164 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098426 [Amblyomma americanum].
ACCESSION   XP_077487178
VERSION     XP_077487178.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631052.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..164
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..164
                     /product="uncharacterized protein LOC144098426"
                     /calculated_mol_wt=18728
     CDS             1..164
                     /gene="LOC144098426"
                     /coded_by="XM_077631052.1:153..647"
                     /db_xref="GeneID:144098426"
ORIGIN      
        1 mspiqgwwpg wrrrntskpq lhllsevwla lrahpkaqgc lvreimavcr sfmdgkyaas
       61 rlrsgaepkf rrfvrywphy fdydreqdti avarhspssa adewvvryla lrieespsgc
      121 laiggrtrcs vghpsshrng tslwspvrrr lasvseaalr rfhs
//
LOCUS       XP_077487244             171 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098442 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077487244
VERSION     XP_077487244.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631118.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..171
                     /product="uncharacterized protein LOC144098442 isoform X1"
                     /calculated_mol_wt=18409
     CDS             1..171
                     /gene="LOC144098442"
                     /coded_by="XM_077631118.1:65..580"
                     /db_xref="GeneID:144098442"
ORIGIN      
        1 mcdypsspdr ptaqlhttgs glhlaiegdh gqgadccrer srdiatcvtr sagklpvaev
       61 akryvasdrr ltptigeqga ddyhhnndyh kgrlgsekrm cgnasyraql savppatrtw
      121 sktstwasri aacavqtpqa kaqaamgrhd ipdavarras apasrcrkwh s
//
LOCUS       XP_077487252             159 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098442 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077487252
VERSION     XP_077487252.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631126.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..159
                     /product="uncharacterized protein LOC144098442 isoform X2"
                     /calculated_mol_wt=16908
     CDS             1..159
                     /gene="LOC144098442"
                     /coded_by="XM_077631126.1:65..544"
                     /db_xref="GeneID:144098442"
ORIGIN      
        1 mcdypsspdr ptaqlhttgs glhlaiegdh gqgadccrer srdiatcvtr sagklpvaev
       61 akryvasdrr ltptigeqga ddyhhnndyh kgrlgsekrm cgnasyraql savppatrtp
      121 mtqssnsfpv eqdidlglph rrlcgsdsag kgpgchgpp
//
LOCUS       XP_077487264             195 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098455 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077487264
VERSION     XP_077487264.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631138.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..195
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..195
                     /product="uncharacterized protein LOC144098455 isoform X1"
                     /calculated_mol_wt=21062
     CDS             1..195
                     /gene="LOC144098455"
                     /coded_by="XM_077631138.1:22..609"
                     /db_xref="GeneID:144098455"
ORIGIN      
        1 mcdypsspdr ptaqlhttgs glhlaiegdh gqgadccrer srdiatcvtr sagklpvaev
       61 akryvasdrr ltltigeqga ddyhhnndyh kgrlgsekrm cgnasyraql savppatrtw
      121 sktstwasri aacavqtpqa kaqaamgrhd ipdavarras apasrcrkwh acalrlasll
      181 arkfvdiqsm fdvss
//
LOCUS       XP_077487272             159 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098455 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077487272
VERSION     XP_077487272.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631146.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..159
                     /product="uncharacterized protein LOC144098455 isoform X2"
                     /calculated_mol_wt=16908
     CDS             1..159
                     /gene="LOC144098455"
                     /coded_by="XM_077631146.1:22..501"
                     /db_xref="GeneID:144098455"
ORIGIN      
        1 mcdypsspdr ptaqlhttgs glhlaiegdh gqgadccrer srdiatcvtr sagklpvaev
       61 akryvasdrr ltltigeqga ddyhhnndyh kgrlgsekrm cgnasyraql savppatrtp
      121 mtqssnsfpv eqdidlglph rrlcgadsag kgpgchgpp
//
LOCUS       XP_077487284             195 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098469 [Amblyomma americanum].
ACCESSION   XP_077487284
VERSION     XP_077487284.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631158.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..195
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..195
                     /product="uncharacterized protein LOC144098469"
                     /calculated_mol_wt=21048
     CDS             1..195
                     /gene="LOC144098469"
                     /coded_by="XM_077631158.1:35..622"
                     /db_xref="GeneID:144098469"
ORIGIN      
        1 mcdypsspdr ptaqlhttgs glhlaiegdh gqgadccrer srdiatcvtr sagklpvaev
       61 akryvasdrr ltltigeqga ddyhhnndyh kgrlgsekrm cgnasyraql savppatrtw
      121 sktstwgsri aacavqtpqa kaqaamgrhd ipdavarras apasrcrkwh acalrlasll
      181 arkfvdiqsm fdvss
//
LOCUS       XP_077487302             129 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098486 [Amblyomma americanum].
ACCESSION   XP_077487302
VERSION     XP_077487302.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631176.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..129
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..129
                     /product="uncharacterized protein LOC144098486"
                     /calculated_mol_wt=13652
     CDS             1..129
                     /gene="LOC144098486"
                     /coded_by="XM_077631176.1:190..579"
                     /db_xref="GeneID:144098486"
ORIGIN      
        1 mpvlanyslp lstgpyfrks pmaspceedi raaaasaata eadialeapl vvngseasqs
       61 hhsvrvesas sssapmtpek atknrslsdg rlaraaeptc rkpwcvgwrt rsmngfatwt
      121 ptydtctfs
//
LOCUS       XP_077487324             164 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098507 [Amblyomma americanum].
ACCESSION   XP_077487324
VERSION     XP_077487324.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631198.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..164
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..164
                     /product="uncharacterized protein LOC144098507"
                     /calculated_mol_wt=17338
     CDS             1..164
                     /gene="LOC144098507"
                     /coded_by="XM_077631198.1:25..519"
                     /db_xref="GeneID:144098507"
ORIGIN      
        1 mhlqamcvhp dlpccsyaql hqsgsgllla mendvgkgad ccrwscriia vcttrpsrhp
       61 laaelakpfv tsvrrltspi gadtasdyhh rrgqgeealk lcegplqtct qpgtavsrpy
      121 sdllangavv eavanearhl ayapcmppca vvtpkapprl psps
//
LOCUS       XP_077487348             161 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098513 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077487348
VERSION     XP_077487348.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631222.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..161
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..161
                     /product="uncharacterized protein LOC144098513 isoform X1"
                     /calculated_mol_wt=17111
     CDS             1..161
                     /gene="LOC144098513"
                     /coded_by="XM_077631222.1:546..1031"
                     /db_xref="GeneID:144098513"
ORIGIN      
        1 mmsrramcvr psspdqptpq lhppgsglhl aiqddvgqga qccpersqdi asrvtrpagk
       61 ppaaevakrf vasvrrlsla feergaddyp hhdyrkgrlg sekhecgnaa crahpsavpp
      121 atgtrgktst wasriaafav qtsqakaqaa itsswrpscc i
//
LOCUS       XP_077487354              73 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098513 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077487354
VERSION     XP_077487354.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631228.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..73
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..73
                     /product="uncharacterized protein LOC144098513 isoform X2"
                     /calculated_mol_wt=7746
     CDS             1..73
                     /gene="LOC144098513"
                     /coded_by="XM_077631228.1:868..1089"
                     /db_xref="GeneID:144098513"
ORIGIN      
        1 mqpagrirqp spqrqarlrr qrprlpsprh ggrvaasrga pgrgaaaapa srchcalqsl
       61 krhdaqqtil aai
//
LOCUS       XP_077487368             194 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098526 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077487368
VERSION     XP_077487368.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631242.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..194
                     /product="uncharacterized protein LOC144098526 isoform X1"
                     /calculated_mol_wt=20522
     CDS             1..194
                     /gene="LOC144098526"
                     /coded_by="XM_077631242.1:40..624"
                     /db_xref="GeneID:144098526"
ORIGIN      
        1 mmsrramcvr psspdqptpq lhppgsglhl aiqddlgqga eccpersqdn asrvtrpagk
       61 ppaaevakrf vasvrrlsla feesgaddyh hhdyrkgrlg sekyecgnaa craqpsavpp
      121 atgtlitqss npfpegkdid lglthrrlcg adfagkgpgc hhlvmaaaap asrchcalqs
      181 lkrpdaqqti laai
//
LOCUS       XP_077487376             170 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098526 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077487376
VERSION     XP_077487376.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631250.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..170
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..170
                     /product="uncharacterized protein LOC144098526 isoform X2"
                     /calculated_mol_wt=18054
     CDS             1..170
                     /gene="LOC144098526"
                     /coded_by="XM_077631250.1:40..552"
                     /db_xref="GeneID:144098526"
ORIGIN      
        1 mmsrramcvr psspdqptpq lhppgsglhl aiqddlgqga eccpersqdn asrvtrpagk
       61 ppaaevakrf vasvrrlsla feesgaddyh hhdyrkgrlg sekyecgnaa craqpsavpp
      121 atgtrgktst wasriaafav qtsqakaqaa itsswrllhl paaatvpcrv
//
LOCUS       XP_077487382             153 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098526 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077487382
VERSION     XP_077487382.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631256.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..153
                     /product="uncharacterized protein LOC144098526 isoform X3"
                     /calculated_mol_wt=16222
     CDS             1..153
                     /gene="LOC144098526"
                     /coded_by="XM_077631256.1:40..501"
                     /db_xref="GeneID:144098526"
ORIGIN      
        1 mmsrramcvr psspdqptpq lhppgsglhl aiqddlgqga eccpersqdn asrvtrpagk
       61 ppaaevakrf vasvrrlsla feesgaddyh hhdyrkgrlg sekyecgnaa craqpsavpp
      121 atgttsqaka qaaitsswrl lhlpaaatvp crv
//
LOCUS       XP_077487395             161 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098539 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077487395
VERSION     XP_077487395.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631269.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..161
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..161
                     /product="uncharacterized protein LOC144098539 isoform X1"
                     /calculated_mol_wt=17158
     CDS             1..161
                     /gene="LOC144098539"
                     /coded_by="XM_077631269.1:44..529"
                     /db_xref="GeneID:144098539"
ORIGIN      
        1 mmsrramcvr psspdqptpq lhppgsglhl aiqddlgqga eccpersqdn asrvtrpagk
       61 ppaaevakrf vasvrrlsla feergaddyh hhdyrkgrlg sekhecgnaa craqpsavpp
      121 atgtrgktst wasriaafav qtsqakaqaa itsswrpscc i
//
LOCUS       XP_077487403             144 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098539 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077487403
VERSION     XP_077487403.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631277.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..144
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..144
                     /product="uncharacterized protein LOC144098539 isoform X2"
                     /calculated_mol_wt=15326
     CDS             1..144
                     /gene="LOC144098539"
                     /coded_by="XM_077631277.1:44..478"
                     /db_xref="GeneID:144098539"
ORIGIN      
        1 mmsrramcvr psspdqptpq lhppgsglhl aiqddlgqga eccpersqdn asrvtrpagk
       61 ppaaevakrf vasvrrlsla feergaddyh hhdyrkgrlg sekhecgnaa craqpsavpp
      121 atgttsqaka qaaitsswrp scci
//
LOCUS       XP_077487416             180 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098552 [Amblyomma americanum].
ACCESSION   XP_077487416
VERSION     XP_077487416.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631290.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..180
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..180
                     /product="uncharacterized protein LOC144098552"
                     /calculated_mol_wt=19225
     CDS             1..180
                     /gene="LOC144098552"
                     /coded_by="XM_077631290.1:35..577"
                     /db_xref="GeneID:144098552"
ORIGIN      
        1 milakeltaa vraraagtsq pmprfhlgsh rtpssqsass prghagpvps eqpvprfqld
       61 rhqtpssksa slprghgapv pseqpvphfh lgshrtpssp sassprghaa pmedktlvtt
      121 tttsvakakr ylssekrlrr narsrsqpla fphylqanda alaprllrsk tlpsaprivt
//
LOCUS       XP_077487429             125 aa            linear   INV 25-NOV-2025
DEFINITION  WD repeat-containing protein 47-like [Amblyomma americanum].
ACCESSION   XP_077487429
VERSION     XP_077487429.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631303.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..125
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..125
                     /product="WD repeat-containing protein 47-like"
                     /calculated_mol_wt=13955
     Region          46..94
                     /region_name="CTLH"
                     /note="C-terminal to LisH motif; smart00668"
                     /db_xref="CDD:128914"
     CDS             1..125
                     /gene="LOC144098558"
                     /coded_by="XM_077631303.1:182..559"
                     /db_xref="GeneID:144098558"
ORIGIN      
        1 maahlclkea evvrlvlefl anwelsisql aleresgvin dalsndllfl rqlildgqwd
       61 nvldfvqple dmeafdskrf kfsvlkhkfl ellcirseag lvqsaelcve lgglpwspga
      121 mrpvs
//
LOCUS       XP_077487439             125 aa            linear   INV 25-NOV-2025
DEFINITION  WD repeat-containing protein 47-like [Amblyomma americanum].
ACCESSION   XP_077487439
VERSION     XP_077487439.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631313.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..125
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..125
                     /product="WD repeat-containing protein 47-like"
                     /calculated_mol_wt=13909
     Region          46..94
                     /region_name="CTLH"
                     /note="C-terminal to LisH motif; smart00668"
                     /db_xref="CDD:128914"
     CDS             1..125
                     /gene="LOC144098567"
                     /coded_by="XM_077631313.1:182..559"
                     /db_xref="GeneID:144098567"
ORIGIN      
        1 maahlclkea evvrlvlefl anwelsisql aleresgvin dalsndllfl rqlildgqwd
       61 nvldfvqple dmeafdskrf kfsvlkhkfl ellcirseag lvqsaelgve lgglpwspga
      121 mrpvs
//
LOCUS       XP_077487452             111 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098574 [Amblyomma americanum].
ACCESSION   XP_077487452
VERSION     XP_077487452.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631326.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..111
                     /product="uncharacterized protein LOC144098574"
                     /calculated_mol_wt=11260
     CDS             1..111
                     /gene="LOC144098574"
                     /coded_by="XM_077631326.1:20..355"
                     /db_xref="GeneID:144098574"
ORIGIN      
        1 mlspsdaril givgedcsvg rrspavvgme paaavspaps papqasqvvd dhvycsvqen
       61 edqpsalatp pptqqaaphq qggpirwlha lhlsskttha vsnkgatgsp l
//
LOCUS       XP_077487466             194 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098578 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077487466
VERSION     XP_077487466.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631340.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..194
                     /product="uncharacterized protein LOC144098578 isoform X1"
                     /calculated_mol_wt=20561
     CDS             1..194
                     /gene="LOC144098578"
                     /coded_by="XM_077631340.1:28..612"
                     /db_xref="GeneID:144098578"
ORIGIN      
        1 mmsrramcvr psspdqptpq lhppgsglhl aiqddlgqga eccpernqdn asrvtrpagk
       61 ppaaevakrf vasvrrlsla feergaddyh hhdyrkgrlg sekhecgnaa craqpsavpp
      121 atgtlitqss npfpegkdid lglthrrlcg adfagkgpgc hhlvmaaell hlggrpaavl
      181 lqhlpaaatv pcrv
//
LOCUS       XP_077487474             161 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098578 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077487474
VERSION     XP_077487474.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631348.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..161
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..161
                     /product="uncharacterized protein LOC144098578 isoform X2"
                     /calculated_mol_wt=17185
     CDS             1..161
                     /gene="LOC144098578"
                     /coded_by="XM_077631348.1:28..513"
                     /db_xref="GeneID:144098578"
ORIGIN      
        1 mmsrramcvr psspdqptpq lhppgsglhl aiqddlgqga eccpernqdn asrvtrpagk
       61 ppaaevakrf vasvrrlsla feergaddyh hhdyrkgrlg sekhecgnaa craqpsavpp
      121 atgtrgktst wasriaafav qtsqakaqaa itsswrpscc i
//
LOCUS       XP_077487486             194 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098591 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077487486
VERSION     XP_077487486.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631360.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..194
                     /product="uncharacterized protein LOC144098591 isoform X1"
                     /calculated_mol_wt=20522
     CDS             1..194
                     /gene="LOC144098591"
                     /coded_by="XM_077631360.1:23..607"
                     /db_xref="GeneID:144098591"
ORIGIN      
        1 mmsrramcvr psspdqptpq lhppgsglhl aiqddlgqga eccpersqdn asrvtrpagk
       61 ppaaevakrf vasvrrlsla feesgaddyh hhdyrkgrlg sekyecgnaa craqpsavpp
      121 atgtlitqss npfpegkdid lglthrrlcg adfagkgpgc hhlvmaaaap asrchcalqs
      181 lkrpdaqqti laai
//
LOCUS       XP_077487494             170 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098591 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077487494
VERSION     XP_077487494.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631368.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..170
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..170
                     /product="uncharacterized protein LOC144098591 isoform X2"
                     /calculated_mol_wt=18054
     CDS             1..170
                     /gene="LOC144098591"
                     /coded_by="XM_077631368.1:23..535"
                     /db_xref="GeneID:144098591"
ORIGIN      
        1 mmsrramcvr psspdqptpq lhppgsglhl aiqddlgqga eccpersqdn asrvtrpagk
       61 ppaaevakrf vasvrrlsla feesgaddyh hhdyrkgrlg sekyecgnaa craqpsavpp
      121 atgtrgktst wasriaafav qtsqakaqaa itsswrllhl paaatvpcrv
//
LOCUS       XP_077487504             153 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098591 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077487504
VERSION     XP_077487504.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631378.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..153
                     /product="uncharacterized protein LOC144098591 isoform X3"
                     /calculated_mol_wt=16222
     CDS             1..153
                     /gene="LOC144098591"
                     /coded_by="XM_077631378.1:23..484"
                     /db_xref="GeneID:144098591"
ORIGIN      
        1 mmsrramcvr psspdqptpq lhppgsglhl aiqddlgqga eccpersqdn asrvtrpagk
       61 ppaaevakrf vasvrrlsla feesgaddyh hhdyrkgrlg sekyecgnaa craqpsavpp
      121 atgttsqaka qaaitsswrl lhlpaaatvp crv
//
LOCUS       XP_077487515             161 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098606 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077487515
VERSION     XP_077487515.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631389.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..161
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..161
                     /product="uncharacterized protein LOC144098606 isoform X1"
                     /calculated_mol_wt=17125
     CDS             1..161
                     /gene="LOC144098606"
                     /coded_by="XM_077631389.1:48..533"
                     /db_xref="GeneID:144098606"
ORIGIN      
        1 mmsrramcvr psspdqptpq lhppgsglhl aiqddlgqga qccpersqdi asrvtrpagk
       61 ppaaevakrf vasvrrlsla feergaddyp hhdyrkgrlg sekhecgnaa crahpsavpp
      121 atgtrgktst wasriaafav qtsqakaqaa itsswrpscc i
//
LOCUS       XP_077487522              73 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098606 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077487522
VERSION     XP_077487522.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631396.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..73
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..73
                     /product="uncharacterized protein LOC144098606 isoform X2"
                     /calculated_mol_wt=7746
     CDS             1..73
                     /gene="LOC144098606"
                     /coded_by="XM_077631396.1:370..591"
                     /db_xref="GeneID:144098606"
ORIGIN      
        1 mqpagrirqp spqrqarlrr qrprlpsprh ggrvaasrga pgrgaaaapa srchcalqsl
       61 krhdaqqtil aai
//
LOCUS       XP_077487552             159 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098633 [Amblyomma americanum].
ACCESSION   XP_077487552
VERSION     XP_077487552.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631426.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..159
                     /product="uncharacterized protein LOC144098633"
                     /calculated_mol_wt=16275
     CDS             1..159
                     /gene="LOC144098633"
                     /coded_by="XM_077631426.1:80..559"
                     /db_xref="GeneID:144098633"
ORIGIN      
        1 mimvkelnaa vraagtsqpa sggqrgshrp lrspsasgtr sdaspftsek ealttttttt
       61 takgawaprs ayagihtagr sgqpspqrqa sgarhrlgph asppvwcrll rqrsrlpslh
      121 hggrvpaprs asgsgaaaap asrfhcvlqs saspdasrt
//
LOCUS       XP_077487563             157 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098643 [Amblyomma americanum].
ACCESSION   XP_077487563
VERSION     XP_077487563.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631437.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..157
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..157
                     /product="uncharacterized protein LOC144098643"
                     /calculated_mol_wt=16222
     CDS             1..157
                     /gene="LOC144098643"
                     /coded_by="XM_077631437.1:182..655"
                     /db_xref="GeneID:144098643"
ORIGIN      
        1 mtkelnaavr aagtsrpasg gqrgshrplr spsasgprsg asplpsekea lttttttata
       61 rgawaprsay aeiqtaersg qpspqrqasg arhrlgphas ppvwcwlhrq rprlpslrhg
      121 grvpatrrps gsgaaaapas riqcvllssa stdasrt
//
LOCUS       XP_077487588             249 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098655 [Amblyomma americanum].
ACCESSION   XP_077487588
VERSION     XP_077487588.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631462.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..249
                     /product="uncharacterized protein LOC144098655"
                     /calculated_mol_wt=27766
     CDS             1..249
                     /gene="LOC144098655"
                     /coded_by="XM_077631462.1:827..1576"
                     /db_xref="GeneID:144098655"
ORIGIN      
        1 mhlvclgvvh kllvlwfrgp kairlgskvr ielsvrnvka apfvpcefnr kprsltdldr
       61 wkatefrffl lyggpvllss llpsqmyenf lalhvaitil smpngtksev qyagqllnhf
      121 vkgftkiygk ehashnvhgl chlasdvehl gpldswsafp fenhmyalkr llrkpeqple
      181 qlsnrlsqqg avlkeaqqtp nfplltgqhe sgpliapcqg pqfkkvkrpg svvlapgkrt
      241 aaafckmdl
//
LOCUS       XP_077487622             780 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme-like 1 [Amblyomma americanum].
ACCESSION   XP_077487622
VERSION     XP_077487622.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631496.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..780
                     /product="endothelin-converting enzyme-like 1"
                     /calculated_mol_wt=87231
     Region          193..>654
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..780
                     /gene="LOC144098680"
                     /coded_by="XM_077631496.1:135..2477"
                     /db_xref="GeneID:144098680"
ORIGIN      
        1 mwrpadgavr grnaaaagae assccsstss gssnsdpghv sdgkrllyll rrpgfkridf
       61 temsellemr raaqrafaar avttvdassq ttelpelglg pqvdsvavrq vgwhmnprfk
      121 kvliatagfc svalfiylvs avwirvryrh lfhatsgtgy idharlplfs gcestacrmy
      181 maaietslnr sqdpcknlyr fvcdgwkqdh rmlsvldaae dlmygralnt iewaprdirk
      241 qgpahsvasi veekvvelak sciepsessl hrlkmfmadh hlpwpmtsrw dlleilldls
      301 gnwnlhlwfq vtldlrpsrk vtgdpvlkig hsaafrawia smrafagqpr rsaqsvryqq
      361 yvrsmlllfd vtesqaddlv tvieamnrlt lavlgpamae pehrtlrmsi rnltktttpg
      421 iaagrlllll nehfiwaqqf ssndivqven pgllravvyl lglksemrea ltlslglrvv
      481 helgwmadqq iadltlelmg lptsvhsrrc lvqiedsvgi awlslfpsnr gfqtlvedvr
      541 dilaeivmrh srsvldvraq gndamwsfla svlpqptpga sfftywlnlm sarwrlqqrn
      601 ltnvlkpgsv lmnrwsvhgp ltvsndysvf plyhpdlppa vnyggagrli adeilrsvfq
      661 dpmygkrsrr rqpspmlhrn eslpaespse wsphhvdaka llaalnayrl gtlrhpsgps
      721 sressraqdr lffvascyal cssgdyvdel ygdasrrcne pvmtlsefta afkcetpnqh
//
LOCUS       XP_077487634             114 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098689 [Amblyomma americanum].
ACCESSION   XP_077487634
VERSION     XP_077487634.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631508.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..114
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..114
                     /product="uncharacterized protein LOC144098689"
                     /calculated_mol_wt=11875
     CDS             1..114
                     /gene="LOC144098689"
                     /coded_by="XM_077631508.1:71..415"
                     /db_xref="GeneID:144098689"
ORIGIN      
        1 malwsaglll laaevallls sdgalgqlka nlcdmdeakl telldcilsn spqgtrdlfe
       61 stkamfggkp paqavlslcs sspaagetli seftnqqtka dkpkfkkalr dcss
//
LOCUS       XP_077487645             233 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098699 [Amblyomma americanum].
ACCESSION   XP_077487645
VERSION     XP_077487645.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631519.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..233
                     /product="uncharacterized protein LOC144098699"
                     /calculated_mol_wt=26106
     CDS             1..233
                     /gene="LOC144098699"
                     /coded_by="XM_077631519.1:1..702"
                     /db_xref="GeneID:144098699"
ORIGIN      
        1 maaykrllvq tevtssssgn csqdivsiln ptttgalvge ssmltdmrrs silqaddhny
       61 tyridcpesl safvgavvpy iagfvvqkvr statcelcia alhsdelapl irqksrggli
      121 spsqdvvglc eavekglwll qaeydtikmv tvrskhlile vlgtcteknw frkledhiid
      181 ldpldnhiyi lckkiaeeyi kvrihhmtke rnreliknkv rpllsrviif nhq
//
LOCUS       XP_077487660             233 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098705 [Amblyomma americanum].
ACCESSION   XP_077487660
VERSION     XP_077487660.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631534.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..233
                     /product="uncharacterized protein LOC144098705"
                     /calculated_mol_wt=26050
     CDS             1..233
                     /gene="LOC144098705"
                     /coded_by="XM_077631534.1:1..702"
                     /db_xref="GeneID:144098705"
ORIGIN      
        1 maaykrllvq tevtssssgn csqdvvsiln ptttgalvge ssmltdmrrs silqaddhny
       61 tyridcpesl safvgavvpy iagfvvqkvr statcelcia alhsdelapl irqksrggli
      121 spsqdvvglc eavenglwll qaeydtikmv tvrskhlile vlgtcteknw fqkledhiid
      181 ldpldnhiyi lckkiaeeyi kvrihhmtke rnreliknkv rpllsrviif nhq
//
LOCUS       XP_077487677             446 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098719 [Amblyomma americanum].
ACCESSION   XP_077487677
VERSION     XP_077487677.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631551.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..446
                     /product="uncharacterized protein LOC144098719"
                     /calculated_mol_wt=51024
     Region          108..196
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     CDS             1..446
                     /gene="LOC144098719"
                     /coded_by="XM_077631551.1:1..1341"
                     /db_xref="GeneID:144098719"
ORIGIN      
        1 myrtpptsrd pspdrkentn vvpdnrashr lqqlppehgl lpaktkkiva eatpmaapas
       61 pivlqqprep pmfrcftfeh peswletyer vatfnswasd dklrhvyfal edaartwfen
      121 reatlttwdl frssflqtft svlrkeraqa lletraqlpn emiaifteem srlfrhadpe
      181 mseekklrll mrgikeelfg amirsppttv eeflreatsi ektlemrnqq fnrrtnspny
      241 agvqslatdd lretiravvr eellrifpss qpqvasvaki vkelqrslgv pevqpqssqp
      301 qpeamtyaai arrqgpppgp rqdpvmpqfr rpppptrppv tqrsyprktd vwrspdhrpl
      361 cyhcgeagqv yrqcpyrdlg lrgfavgapr prggerprdi adylaatqws prrpsrspsp
      421 grylspqrrp ytgpvrgraa spypen
//
LOCUS       XP_077487689             114 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098723 [Amblyomma americanum].
ACCESSION   XP_077487689
VERSION     XP_077487689.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631563.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..114
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..114
                     /product="uncharacterized protein LOC144098723"
                     /calculated_mol_wt=12496
     CDS             1..114
                     /gene="LOC144098723"
                     /coded_by="XM_077631563.1:349..693"
                     /db_xref="GeneID:144098723"
ORIGIN      
        1 milnlkaqan krvedmvkds iransnqsqs vsiaercasl eaelsslapv eclscqdlrl
       61 elleeeaaar vatwehvlaq pqamrclspg rlcgsqrstt clghwapwhl vrrg
//
LOCUS       XP_077487740             168 aa            linear   INV 25-NOV-2025
DEFINITION  spindle assembly abnormal protein 6 homolog [Amblyomma americanum].
ACCESSION   XP_077487740
VERSION     XP_077487740.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631614.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..168
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..168
                     /product="spindle assembly abnormal protein 6 homolog"
                     /calculated_mol_wt=18830
     Region          24..153
                     /region_name="HD_SAS6_N"
                     /note="N-terminal head domain found in spindle assembly
                     abnormal protein 6 and similar proteins; cd10142"
                     /db_xref="CDD:408998"
     Site            74..75
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:408998"
     CDS             1..168
                     /gene="LOC144098757"
                     /coded_by="XM_077631614.1:49..555"
                     /db_xref="GeneID:144098757"
ORIGIN      
        1 mildsqyrra dgvstresgq deslycrtls vrlkddfcat arrvssmclt vrksksstvg
       61 rkelqiqltd dadpffvytl tlteedfqsl karqgllmdf lsfpqkfvdl lelsskddqk
      121 enprfvvslv vqgrtgsdpa ildiveanpf thldssslvh qtrssari
//
LOCUS       XP_077487770             159 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098776 [Amblyomma americanum].
ACCESSION   XP_077487770
VERSION     XP_077487770.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631644.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..159
                     /product="uncharacterized protein LOC144098776"
                     /calculated_mol_wt=16587
     CDS             1..159
                     /gene="LOC144098776"
                     /coded_by="XM_077631644.1:175..654"
                     /db_xref="GeneID:144098776"
ORIGIN      
        1 mildkelnva lraagtsqpa sggqweshqp qtspsssgtr sgasplpsek ealttttttt
       61 tardawaprs acagmqtagr scqpspqrqa cearhwlgph asspvwcrlr rqrprlpslr
      121 hggrvpaprr vsgsggaaap asrihcvlls sasphasrt
//
LOCUS       XP_077487796             159 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098796 [Amblyomma americanum].
ACCESSION   XP_077487796
VERSION     XP_077487796.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631670.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..159
                     /product="uncharacterized protein LOC144098796"
                     /calculated_mol_wt=16485
     CDS             1..159
                     /gene="LOC144098796"
                     /coded_by="XM_077631670.1:136..615"
                     /db_xref="GeneID:144098796"
ORIGIN      
        1 mildkelnva lraagtsqpa sggqweshqp qtspsssgtr sgasplpsek ealttttttt
       61 tardawaprs acagmqtagr scqpspqrqa cgarhrlgph asspvwcrlr rqrprlpslr
      121 hggrvpaprr vsgsggaaap asrihcvlls sasphasrt
//
LOCUS       XP_077487820             150 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098812 [Amblyomma americanum].
ACCESSION   XP_077487820
VERSION     XP_077487820.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631694.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..150
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..150
                     /product="uncharacterized protein LOC144098812"
                     /calculated_mol_wt=16755
     CDS             1..150
                     /gene="LOC144098812"
                     /coded_by="XM_077631694.1:161..613"
                     /db_xref="GeneID:144098812"
ORIGIN      
        1 mtscyqklqs allykavkdi scsdsgscgt hqrslcnsnc fmkhiqrtvl ccenqiifse
       61 asdfteaact afilyyimnq eypegaaisl kfvqrqfdln lpnesrqkdv krsrisvpsk
      121 imnlvnklgl qvetvnpcgv rksdatqehs
//
LOCUS       XP_077487839             159 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098820 [Amblyomma americanum].
ACCESSION   XP_077487839
VERSION     XP_077487839.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631713.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..159
                     /product="uncharacterized protein LOC144098820"
                     /calculated_mol_wt=16485
     CDS             1..159
                     /gene="LOC144098820"
                     /coded_by="XM_077631713.1:136..615"
                     /db_xref="GeneID:144098820"
ORIGIN      
        1 mildkelnva lraagtsqpa sggqweshqp qtspsssgtr sgasplpsek ealttttttt
       61 tardawaprs acagmqtagr scqpspqrqa cgarhrlgph asspvwcrlr rqrprlpslr
      121 hggrvpaprr vsgsggaaap asrihcvlls sasphasrt
//
LOCUS       XP_077487851             132 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098826 [Amblyomma americanum].
ACCESSION   XP_077487851
VERSION     XP_077487851.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631725.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..132
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..132
                     /product="uncharacterized protein LOC144098826"
                     /calculated_mol_wt=14402
     CDS             1..132
                     /gene="LOC144098826"
                     /coded_by="XM_077631725.1:3..401"
                     /db_xref="GeneID:144098826"
ORIGIN      
        1 mrrramcvhp scpdrptaql hpprsglhla mqddlgqgak rccearrnia nrvrrpagep
       61 paaevakrfg dsvrrlalaf gergaddyhh hdyrkgrlgs ekrvcgnvdc raqpsavple
      121 tgkwsktsag ap
//
LOCUS       XP_077487904             193 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098860 [Amblyomma americanum].
ACCESSION   XP_077487904
VERSION     XP_077487904.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631778.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..193
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..193
                     /product="uncharacterized protein LOC144098860"
                     /calculated_mol_wt=21486
     Region          <5..167
                     /region_name="transpos_IS1595"
                     /note="IS1595 family transposase; NF033547"
                     /db_xref="CDD:468078"
     CDS             1..193
                     /gene="LOC144098860"
                     /coded_by="XM_077631778.1:1..582"
                     /db_xref="GeneID:144098860"
ORIGIN      
        1 mggpgevvqv declmrgkrk anrgrllagd nlpphrqdny ggvvdrgpwv fgmiweatke
       61 lrlfkvdrrd aatlgaiiaa nvqsgttivs desvayqcvp glldsngvnm nlthqtvnhs
      121 qffvdpvtgt htqsiesswq kakarlirsg rkltpqltea ylgwlwwqsi ngrqrchdpf
      181 lrlmeaiakh ysm
//
LOCUS       XP_077487955             103 aa            linear   INV 25-NOV-2025
DEFINITION  defensin-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077487955
VERSION     XP_077487955.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631829.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..103
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..103
                     /product="defensin-like isoform X1"
                     /calculated_mol_wt=11658
     Region          <64..96
                     /region_name="Defensin_2"
                     /note="Arthropod defensin; cl03093"
                     /db_xref="CDD:470736"
     CDS             1..103
                     /gene="LOC144098892"
                     /coded_by="XM_077631829.1:30..341"
                     /db_xref="GeneID:144098892"
ORIGIN      
        1 memfsassnv kkrtrennii qqgemkllav afvfvlvagv lskdfveegk aevsrvrvrr
       61 tlfcdpfyre acdrychslr lqtgycggvf thictcwrll lsg
//
LOCUS       XP_077487971              79 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098892 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077487971
VERSION     XP_077487971.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631845.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..79
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..79
                     /product="uncharacterized protein LOC144098892 isoform X2"
                     /calculated_mol_wt=8961
     CDS             1..79
                     /gene="LOC144098892"
                     /coded_by="XM_077631845.1:30..269"
                     /db_xref="GeneID:144098892"
ORIGIN      
        1 memfsassnv kkrtrennii qqgemkllav afvfvlvaei rtshfspmpr lspqayghng
       61 prhggyrspv erfcrrrqs
//
LOCUS       XP_077487980              68 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098892 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077487980
VERSION     XP_077487980.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631854.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..68
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..68
                     /product="uncharacterized protein LOC144098892 isoform X3"
                     /calculated_mol_wt=7580
     CDS             1..68
                     /gene="LOC144098892"
                     /coded_by="XM_077631854.1:30..236"
                     /db_xref="GeneID:144098892"
ORIGIN      
        1 memfsassnv kkrtrennii qqgemkllav afvfvlvagv wtqwasarri qescrkilsk
       61 kaklkspa
//
LOCUS       XP_077487997             735 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077487997
VERSION     XP_077487997.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631871.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..735
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=83144
     Region          73..199
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          291..674
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..735
                     /gene="LOC144098915"
                     /coded_by="XM_077631871.1:192..2399"
                     /db_xref="GeneID:144098915"
ORIGIN      
        1 msfwklrpvv ggalllyaal scnalsasag elhrktgats smtdslramv dalldpsspl
       61 arkllradms ptcslgllrl vrgirdlepw alrivdssgk yptgflhgts vdlgaydecv
      121 atvvrdeydy iqtrgqycvl qlqlmgndsa veyfvpaatm vhkrasnfis ywtdplapga
      181 rigicivdrc seediealar tvlpdtlkvt vsdcvtseyp sisptqagii cflsciaamm
      241 ffstllhvyl wfknrsakeg qvirlltsfs vlrntqyivs ldekssdens ftfmngmrfl
      301 siawiilghv yggmtdilag rtnmlfffdy wpgailpagf lsvdtflfls gfllsnavfk
      361 lkkppvliva iaavrryirv vvpvafllac mqllplavqg psttryyrei qtmiekhwyh
      421 fllqvfnfkh elhsialvhi wyvsadfqlf lvslavllif rwskfwcgaa ffvlsvlscv
      481 vsmlqvhktd lppfmvgmpe nfsttrdiln nyyvqpfyha vcffsgcitw llkdsfnsrp
      541 fpkaatiicw ilsigcgltc vfikvpwyqa rdptgeagkl cmaffdrilw aiclswitla
      601 cstkrggimd sflswkgfep lsklaftvyl vhmplydlmn hyarertyfs hfslvtqffs
      661 vllwsyflgy llsisceapl ghidrflfsa rrkppqvies kqvdevlmee kkhsffvits
      721 ngifrrreee skvie
//
LOCUS       XP_077488018             481 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098926 [Amblyomma americanum].
ACCESSION   XP_077488018
VERSION     XP_077488018.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631892.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..481
                     /product="uncharacterized protein LOC144098926"
                     /calculated_mol_wt=53827
     Region          64..421
                     /region_name="transpos_IS630"
                     /note="IS630 family transposase; NF033545"
                     /db_xref="CDD:468076"
     CDS             1..481
                     /gene="LOC144098926"
                     /coded_by="XM_077631892.1:283..1728"
                     /db_xref="GeneID:144098926"
ORIGIN      
        1 maslsparlf sspastskts pvrlfskvks pkfskdckav ivnvyasirk lhsektvrdv
       61 laivgemtgv sprtvvsfkk eglsggvksp qkrpkrmais ssrsvkydsf tvnairlklh
      121 rmyaerqipt ldsilhevng dddlpdmska tlwrllkemg fkfekrkrtl aliersdiva
      181 wrrrylraik afrsqgrciv yldetwvnag htrqhvwkds tvkssqdafl rglttglpap
      241 sgkggrlilv hagssltgfv sgaadffrak kgasadyhse mdgkyfeewf sskllpnipn
      301 ssiivmdnap yhsvalekpl ttstrkieiq awltrkgifw stdmvraell dlckrttsgh
      361 tvyaidtlaa khghevlrlp pyhcefnpie qvwsmvkgfv akrnkaftla eveellpqal
      421 ahvskedwqk fcahaeried eawerdiicd devdpvvfai gssssssees sselsgieel
      481 e
//
LOCUS       XP_077488032             218 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098932 [Amblyomma americanum].
ACCESSION   XP_077488032
VERSION     XP_077488032.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631906.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..218
                     /product="uncharacterized protein LOC144098932"
                     /calculated_mol_wt=25180
     Region          11..>121
                     /region_name="HOOK"
                     /note="HOOK protein coiled-coil region; pfam05622"
                     /db_xref="CDD:461694"
     CDS             1..218
                     /gene="LOC144098932"
                     /coded_by="XM_077631906.1:73..729"
                     /db_xref="GeneID:144098932"
ORIGIN      
        1 mmaisdkldq llplketidg iedslqllsd qyddllsrte rnerevkelr krvekvetqn
       61 ddvtqlkddi ddlewrsrrl nlefhgiqet dkqnlldkin altaqiklpa lsendvvavh
      121 rlpakkdkip giicrfarqa drdawwqnrk klqeadgnif vlenltkrtr allfeaknwa
      181 kvkkykyvwh sngrvlvrka dglnavlvan vgdlekla
//
LOCUS       XP_077488061             383 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098946 [Amblyomma americanum].
ACCESSION   XP_077488061
VERSION     XP_077488061.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631935.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..383
                     /product="uncharacterized protein LOC144098946"
                     /calculated_mol_wt=41598
     Region          <12..42
                     /region_name="F-box_SF"
                     /note="F-box domain superfamily; cl45894"
                     /db_xref="CDD:459239"
     Site            order(12,15..16,19..20,24,26..27,31..33,35)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438903"
     CDS             1..383
                     /gene="LOC144098946"
                     /coded_by="XM_077631935.1:106..1257"
                     /db_xref="GeneID:144098946"
ORIGIN      
        1 mdvssvvsqn fvvesvlnhl dtedlftcae vsrqwqsval sllrrklisv svccardpee
       61 lhvmppepsm hscvdifctk farfrniarm agmhpslvfi schadlnedk rvveclrell
      121 ppdcvvvyfk lelvrgpdsd seavshegvf geflfrteal prvlpsleds egsesqqqpp
      181 dapiaarpss pqlqgrpddd eppataasaa aatatataet aagelvraea apeplvwprp
      241 rlpsvgsngg avfhnvlpgv lmfqalhvte asvegnktts rfmeeatrkt iqvtsaamys
      301 tmpstslqpf mrnlsegfgp ttntiavafp rnqdearael daferafpnv palanqatef
      361 nvdgqyaplg rlklilllik lld
//
LOCUS       XP_077488078             383 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144098961 [Amblyomma americanum].
ACCESSION   XP_077488078
VERSION     XP_077488078.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631952.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..383
                     /product="uncharacterized protein LOC144098961"
                     /calculated_mol_wt=41571
     Region          12..46
                     /region_name="F-box_SF"
                     /note="F-box domain superfamily; cl45894"
                     /db_xref="CDD:459239"
     Site            order(12,15..16,19..20,24,26..27,31..33,35)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438852"
     CDS             1..383
                     /gene="LOC144098961"
                     /coded_by="XM_077631952.1:109..1260"
                     /db_xref="GeneID:144098961"
ORIGIN      
        1 mdvssvvsqn fvvenvlnhl dtedlftcae vsrlwqsval sllrrklisv svccardpee
       61 lhvmppepsm hscvdifctk farfrniarm agmhpslvfi schadlnedk rvveclrell
      121 ppdcvvvyfk lelvrgpdss seevshegif geflfrteal prvlssleds dgsesqqqpp
      181 daptaarpss pqlqgrtddd eppatassaa aatatataet aagelvraea apeplvwprp
      241 rlpsvgsngg avfhnvlpgv lmfqalhvte asvegnatts rfmeeatrkt iqvtsaamys
      301 tmsstslqpf mrnlsegfgp ttntiavafp rnqdearael daferafpnv palanqatef
      361 nvdgqyaplg rlklilllik lld
//
LOCUS       XP_077488096             453 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-(1,3)-fucosyltransferase C-like [Amblyomma americanum].
ACCESSION   XP_077488096
VERSION     XP_077488096.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077631970.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..453
                     /product="alpha-(1,3)-fucosyltransferase C-like"
                     /calculated_mol_wt=51362
     Region          115..243
                     /region_name="Glyco_tran_10_N"
                     /note="Fucosyltransferase, N-terminal; pfam17039"
                     /db_xref="CDD:465336"
     Region          <324..428
                     /region_name="Glyco_transf_10"
                     /note="Glycosyltransferase family 10 (fucosyltransferase)
                     C-term; pfam00852"
                     /db_xref="CDD:459962"
     CDS             1..453
                     /gene="LOC144098979"
                     /coded_by="XM_077631970.1:119..1480"
                     /db_xref="GeneID:144098979"
ORIGIN      
        1 mlprraedir wprlhailrp wdvlfdylhv fttnvrgrll vilmvalfav atvlllrals
       61 thsregkygg eykpltpapt psedeshrrr gtstvstqlg nvlttpssvs tlnlrrpril
      121 lwtkpgqrrp vegmhlpttg ettlrycsfp vrhampkvpl etfppgcyvt rdrslfnvsd
      181 avvfdasavh gtippklris nqvwvfwthl gsaptedlgs mttdafnwtm grrmdadvvv
      241 pfghwtqser agsrdfakkq htkkgtalff tsdcdrkedm evltevmdal qgstvrgcga
      301 secgwlqlcl geyagdfhfl fvaessgcye hpleaiydaf rydlvpvyfg hkiqaglpeh
      361 sfvdafamlp atdiidylta lvkdsdlysa ffkwkedhal srlddlcylc dalrknlqrq
      421 tndvvawwkk snlcrlnhrf nqvhsqphea yih
//
LOCUS       XP_077488136             159 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger protein 711-like [Amblyomma americanum].
ACCESSION   XP_077488136
VERSION     XP_077488136.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632010.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..159
                     /product="zinc finger protein 711-like"
                     /calculated_mol_wt=17807
     CDS             1..159
                     /gene="LOC144099014"
                     /coded_by="XM_077632010.1:81..560"
                     /db_xref="GeneID:144099014"
ORIGIN      
        1 maeiqvrhes edgttdsdls pherreitiq qriledlrpa gdmtvhrcev cdknfhlpsk
       61 liahyayrhk revatssgkk lpcplceapc ksrrtmalhv gmdlgerlct kcgaefacaa
      121 taaahkhfhk sgghfmcgsc kklfaynsdr eehvrvehp
//
LOCUS       XP_077488148             190 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099021 [Amblyomma americanum].
ACCESSION   XP_077488148
VERSION     XP_077488148.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632022.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..190
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..190
                     /product="uncharacterized protein LOC144099021"
                     /calculated_mol_wt=21064
     CDS             1..190
                     /gene="LOC144099021"
                     /coded_by="XM_077632022.1:13..585"
                     /db_xref="GeneID:144099021"
ORIGIN      
        1 mdasqpstsr stprsssmad tqarhesedg ttdsdlspee rseitikrrv ledlrpagdm
       61 tvhrceicdk nfhllskfia hnayrhhrka atspgkklsc plceapcnsr ttmalhagth
      121 lgerlctkcg aefacaataa ahehfhksrg hfmcgscktl faynsdreeh irvehpqhrg
      181 lkrprcpstd
//
LOCUS       XP_077488162             190 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099028 [Amblyomma americanum].
ACCESSION   XP_077488162
VERSION     XP_077488162.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632036.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..190
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..190
                     /product="uncharacterized protein LOC144099028"
                     /calculated_mol_wt=21064
     CDS             1..190
                     /gene="LOC144099028"
                     /coded_by="XM_077632036.1:13..585"
                     /db_xref="GeneID:144099028"
ORIGIN      
        1 mdasqpstsr stprsssmad tqarhesedg ttdsdlspee rseitikrrv ledlrpagdm
       61 tvhrceicdk nfhllskfia hnayrhhrka atspgkklsc plceapcnsr ttmalhagth
      121 lgerlctkcg aefacaataa ahehfhksrg hfmcgscktl faynsdreeh irvehpqhrg
      181 lkrprcpstd
//
LOCUS       XP_077488192             176 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099044 [Amblyomma americanum].
ACCESSION   XP_077488192
VERSION     XP_077488192.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632066.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..176
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..176
                     /product="uncharacterized protein LOC144099044"
                     /calculated_mol_wt=19520
     CDS             1..176
                     /gene="LOC144099044"
                     /coded_by="XM_077632066.1:57..587"
                     /db_xref="GeneID:144099044"
ORIGIN      
        1 mdasqpstsr stprspsmsd tqarhesedg ttdsdlspee rreitiqqri ledlrpaedm
       61 tvhrcevcek nfhlpsklia hyayrhkrea atssgkklac plceapcnsr ttmalhvgmh
      121 lgerlctkcg aefacaataa ahkhfhksgg hfmcgsckkl faynsdreeh vrvehp
//
LOCUS       XP_077488205             185 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099051 [Amblyomma americanum].
ACCESSION   XP_077488205
VERSION     XP_077488205.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632079.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..185
                     /product="uncharacterized protein LOC144099051"
                     /calculated_mol_wt=20340
     Region          61..82
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(61,64,77,82)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(66,68,70,72..73,76..77,81,101,103,107..108,111..112,
                     115,127,129,131,133..134,137..138,141)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          96..116
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          122..142
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..185
                     /gene="LOC144099051"
                     /coded_by="XM_077632079.1:109..666"
                     /db_xref="GeneID:144099051"
ORIGIN      
        1 mdpeqpstsy sttmsgrdgs vddsfapdls sgdasptygi avqpkipenv rpagdittyc
       61 cdscdkdfhl pskliahivy rherygarpy arraacflcq apcksraamv lhmgshlger
      121 cckkcgaefa caateaahkh fhktggyfmc gfceklfark gdreehtkle hpqrplnrpr
      181 spaie
//
LOCUS       XP_077488214             185 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099051 [Amblyomma americanum].
ACCESSION   XP_077488214
VERSION     XP_077488214.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632088.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..185
                     /product="uncharacterized protein LOC144099051"
                     /calculated_mol_wt=20340
     Region          61..82
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(61,64,77,82)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(66,68,70,72..73,76..77,81,101,103,107..108,111..112,
                     115,127,129,131,133..134,137..138,141)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          96..116
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          122..142
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..185
                     /gene="LOC144099051"
                     /coded_by="XM_077632088.1:111..668"
                     /db_xref="GeneID:144099051"
ORIGIN      
        1 mdpeqpstsy sttmsgrdgs vddsfapdls sgdasptygi avqpkipenv rpagdittyc
       61 cdscdkdfhl pskliahivy rherygarpy arraacflcq apcksraamv lhmgshlger
      121 cckkcgaefa caateaahkh fhktggyfmc gfceklfark gdreehtkle hpqrplnrpr
      181 spaie
//
LOCUS       XP_077488229             419 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099058 [Amblyomma americanum].
ACCESSION   XP_077488229
VERSION     XP_077488229.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632103.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..419
                     /product="uncharacterized protein LOC144099058"
                     /calculated_mol_wt=44064
     CDS             1..419
                     /gene="LOC144099058"
                     /coded_by="XM_077632103.1:152..1411"
                     /db_xref="GeneID:144099058"
ORIGIN      
        1 msqgkdatss aktdpsdkrc tcpgdvpatt arkgkqrkpa ntanqegfli rlvpspghdl
       61 salrhppapq rvlpqpaaqp ppavllvnqp pmalvppfag apyltspsyl plapgcqpls
      121 vtavanaphs vvgndcggch nrcshrhccs agsrgdysaq ntltippyam pffgevyrsa
      181 aaaqapqagg gnvnvvtkvi sddqkrkrrg kkqrrdsssd ssdnyskkrr ksgpttrkde
      241 pqivvvpsaa spasppppre epapaspppv tspppethmp fvaspgpmmy pqspedqhty
      301 dnmpsrqgde enaegeeekg pnpmacffvv vvvgvcvvia ivlalypsil fgkkgngdet
      361 taeargllgr aatvlgdlkg rvtnrggtvg ytavhssekl attsdaeven lagtlwrrt
//
LOCUS       XP_077488244             167 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099062 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077488244
VERSION     XP_077488244.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632118.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..167
                     /product="uncharacterized protein LOC144099062 isoform X1"
                     /calculated_mol_wt=19118
     CDS             1..167
                     /gene="LOC144099062"
                     /coded_by="XM_077632118.1:357..860"
                     /db_xref="GeneID:144099062"
ORIGIN      
        1 mrrhhrrqtr arklfpsrvs syidycfsdv aappkknipv eepslegtke vvvaelqlla
       61 sdtlsgvtyn tervmcflkv tfqehcqhyv tlcqviswek issrqlcaea qlrfestvdk
      121 levrlkkaaq vfseqqgqdt slvqisrhlq gsldhflite allqqri
//
LOCUS       XP_077488249             167 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099062 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077488249
VERSION     XP_077488249.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632123.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..167
                     /product="uncharacterized protein LOC144099062 isoform X1"
                     /calculated_mol_wt=19118
     CDS             1..167
                     /gene="LOC144099062"
                     /coded_by="XM_077632123.1:276..779"
                     /db_xref="GeneID:144099062"
ORIGIN      
        1 mrrhhrrqtr arklfpsrvs syidycfsdv aappkknipv eepslegtke vvvaelqlla
       61 sdtlsgvtyn tervmcflkv tfqehcqhyv tlcqviswek issrqlcaea qlrfestvdk
      121 levrlkkaaq vfseqqgqdt slvqisrhlq gsldhflite allqqri
//
LOCUS       XP_077488258             150 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099062 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077488258
VERSION     XP_077488258.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632132.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..150
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..150
                     /product="uncharacterized protein LOC144099062 isoform X2"
                     /calculated_mol_wt=16215
     CDS             1..150
                     /gene="LOC144099062"
                     /coded_by="XM_077632132.1:136..588"
                     /db_xref="GeneID:144099062"
ORIGIN      
        1 mvvctvdmae drlrcdycdy cdhslsthsr ivdlgtimqd teqqsnafss vlsssqpgcs
       61 kdswpctsee spvqcedite grqepescsh qapvqdahgv tstqavsnqc tdpprtarsa
      121 smsahcaglq rgisgqilks ideridldmk
//
LOCUS       XP_077488293             416 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099080 [Amblyomma americanum].
ACCESSION   XP_077488293
VERSION     XP_077488293.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632167.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..416
                     /product="uncharacterized protein LOC144099080"
                     /calculated_mol_wt=45114
     Region          367..401
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..416
                     /gene="LOC144099080"
                     /coded_by="XM_077632167.1:146..1396"
                     /db_xref="GeneID:144099080"
ORIGIN      
        1 maaqgsreqs qysrrssrsq rrgrlprala aedrlseara srelpppppp pppqqhpwad
       61 srqsagrsta sslisrlgkh ssrppssmld eeaamrrsat svagaasgmt pvrltryygp
      121 prlgrerqee eaaefgphvd gsrtwevgge mpgrrldrap laadaggsrv srrsasrqar
      181 tvaecvapas tpprparskd agdrphrtle mtfamgafvc vatlvvafvv rawmpyears
      241 dpfcrsdacl ahvrlieari drgvdpcvdf dayacsrwkp asefyghasa ltnvlldnwr
      301 rstlnaaapa dvevkqeqpg edaemreaav aaaaappqpw plamrpaavm phrlaaggaa
      361 aqalssdpee lfclslaarl krllprerhm armkmlqtlh dlesgenige falgdp
//
LOCUS       XP_077488302             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099088 [Amblyomma americanum].
ACCESSION   XP_077488302
VERSION     XP_077488302.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632176.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144099088"
                     /calculated_mol_wt=24232
     Region          7..91
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     Region          168..202
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..217
                     /gene="LOC144099088"
                     /coded_by="XM_077632176.1:286..939"
                     /db_xref="GeneID:144099088"
ORIGIN      
        1 masfneklvq evkkhtqlwd qhsklhkeag yreaawmdia aalavtveec qtrwrtlrdt
       61 ylkrkkrrra datgqwsvld relgflddfl rprtrrstln gaapvassae vevkreqpde
      121 daevresaav vaaaaapaqp walamrptrv mphrlaagga aaqalssdpe elfclslaar
      181 lkrllprerh marmkmlqtl hdlqsgenig efalgdp
//
LOCUS       XP_077488311             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099097 [Amblyomma americanum].
ACCESSION   XP_077488311
VERSION     XP_077488311.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632185.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144099097"
                     /calculated_mol_wt=24073
     Region          7..91
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     Region          168..202
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..217
                     /gene="LOC144099097"
                     /coded_by="XM_077632185.1:60..713"
                     /db_xref="GeneID:144099097"
ORIGIN      
        1 masfneklvq evkkhtqlwd qhsklhkeag yreaawmdia aalavtveec qtrwrtlrdt
       61 ylkrkkrrra datgqwsvld relgflddfl rprtrrstln gaapaassae vevkreqpde
      121 daevresaav vaaaaapaqp walamrpaav mphrlaagga aaqalsldpe elfclslaal
      181 lkrllprerh marmkmlqtl hdlesgenig efalgdp
//
LOCUS       XP_077488323             211 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099105 [Amblyomma americanum].
ACCESSION   XP_077488323
VERSION     XP_077488323.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632197.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..211
                     /product="uncharacterized protein LOC144099105"
                     /calculated_mol_wt=23533
     Region          7..91
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     Region          162..196
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..211
                     /gene="LOC144099105"
                     /coded_by="XM_077632197.1:26..661"
                     /db_xref="GeneID:144099105"
ORIGIN      
        1 masfneklvq evkkhtqlwd qhsklhkeag yreaawmeia aalavtveec qtrlrtlrdt
       61 ylkrkkrrra gangqwnvle rdlgflddfl rprtrrstln aaapadvevk qeqpgedaem
      121 reaavaaaaa ppqpwplamr paavmphrla aggaaaqals sdpeelfcli laarlkrllp
      181 rkrhmarmkm lqtlhdlesg enigefalgd p
//
LOCUS       XP_077488349             223 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099126 [Amblyomma americanum].
ACCESSION   XP_077488349
VERSION     XP_077488349.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632223.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..223
                     /product="uncharacterized protein LOC144099126"
                     /calculated_mol_wt=20116
     CDS             1..223
                     /gene="LOC144099126"
                     /coded_by="XM_077632223.1:143..814"
                     /db_xref="GeneID:144099126"
ORIGIN      
        1 mkfivaafva alafvsaeek dvegrgailg gpgvvgagyg pglvgaanpa vlgapgagyv
       61 ggpglvagpg vvapgvvapg vvapgviapg vgapglvnpg lvggvgyglg gvgygvgggy
      121 gaaqgvaagg hqagfqkgaa ghaqgsgaya ggaaqknvqa ynnqagyshn sgfassdtkt
      181 fgagqqqgst gyvggaaghq agfgktahga aggvggvgfg ihg
//
LOCUS       XP_077488362             263 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099136 [Amblyomma americanum].
ACCESSION   XP_077488362
VERSION     XP_077488362.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632236.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..263
                     /product="uncharacterized protein LOC144099136"
                     /calculated_mol_wt=29331
     Region          230..>260
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     CDS             1..263
                     /gene="LOC144099136"
                     /coded_by="XM_077632236.1:1..792"
                     /db_xref="GeneID:144099136"
ORIGIN      
        1 mdvvhpsyvs vddftgevsw ikqaveehsv clpiarviic gpfgelitea avskavplqy
       61 pylfsnrsdq llrergqklg sgviqaltrs ktrqlasqls qipepqaard apasislqsn
      121 eegseptrne sqtadraner rgtsttksve esenaegsvl sptsrsfdrl lqvnresltk
      181 eqkqdptler lhltakegia rrnitmheeg gllyrhyqdr kgktfdqlvv pekyradils
      241 lchghgwtgh lvinktkdrl lme
//
LOCUS       XP_077488371             270 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099141 [Amblyomma americanum].
ACCESSION   XP_077488371
VERSION     XP_077488371.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632245.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..270
                     /product="uncharacterized protein LOC144099141"
                     /calculated_mol_wt=24069
     Region          <171..>254
                     /region_name="ser_rich_anae_1"
                     /note="serine-rich protein; NF033849"
                     /db_xref="CDD:468206"
     CDS             1..270
                     /gene="LOC144099141"
                     /coded_by="XM_077632245.1:98..910"
                     /db_xref="GeneID:144099141"
ORIGIN      
        1 mkifaiavvt alvscavfae datknkdskk dekkdvearg gilgggvglg gvggygagmd
       61 pglvgaglgg aglvgaaigg aglegvglge aglagasygg aglagaglgg aglagaglgg
      121 aglagaglgs palvgtgvag pgvvgsgvvg tprlvgagig sgigggfqsg ygssaggrqs
      181 gfqggasghn qgsgafagga ssskvnafnn nrgyshssgf sasdsktfgs ghkqgssgfn
      241 ngasghqagf gqssfgkagg vgaggvglhg
//
LOCUS       XP_077488384             263 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099146 [Amblyomma americanum].
ACCESSION   XP_077488384
VERSION     XP_077488384.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632258.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..263
                     /product="uncharacterized protein LOC144099146"
                     /calculated_mol_wt=23288
     Region          <164..>251
                     /region_name="ser_rich_anae_1"
                     /note="serine-rich protein; NF033849"
                     /db_xref="CDD:468206"
     CDS             1..263
                     /gene="LOC144099146"
                     /coded_by="XM_077632258.1:138..929"
                     /db_xref="GeneID:144099146"
ORIGIN      
        1 mkifaitvvt alvacavfaq datknkdskk dekkdvearg gilgggvglg gvggygagvg
       61 pgliaaglgg aglggaglag asyggaglag aglggaglgg aglggaglag agiggaglag
      121 aglgspalvg tgvagpgvvg sgvvgtpglv gagigrgigg gfqsgygssa gghrsgfqgg
      181 asghnqgsga faggassskv nafnnnrgys hssgfsasds ktfgsghqqg ssgfnngasg
      241 hqagfgqssf gkaggvggvg lhg
//
LOCUS       XP_077488398             177 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099152 [Amblyomma americanum].
ACCESSION   XP_077488398
VERSION     XP_077488398.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632272.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..177
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..177
                     /product="uncharacterized protein LOC144099152"
                     /calculated_mol_wt=18109
     CDS             1..177
                     /gene="LOC144099152"
                     /coded_by="XM_077632272.1:149..682"
                     /db_xref="GeneID:144099152"
ORIGIN      
        1 mkffatlavm gvmactafae eviikdvkdv kedpeepgvl lggydvvvvp nlvapglvgy
       61 palvgpglgy gidfqsgyda ftrghksgfq kdaaahnqgs sdfargtlhr tysayknnqg
      121 yshssgftat dsksygagrq qassghkgge aghqsgfgqs sfgkaagvgp aavalqv
//
LOCUS       XP_077488408             199 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099157 [Amblyomma americanum].
ACCESSION   XP_077488408
VERSION     XP_077488408.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632282.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..199
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..199
                     /product="uncharacterized protein LOC144099157"
                     /calculated_mol_wt=18154
     CDS             1..199
                     /gene="LOC144099157"
                     /coded_by="XM_077632282.1:120..719"
                     /db_xref="GeneID:144099157"
ORIGIN      
        1 mkfysptlli afvacvalae eaakkddkkd vearggvlgg lggvgygagl gtglgaglvg
       61 tglggvglgg aglagpglvg pglvgaglgh glghgfqsgy gssaggqqgg yqggaaghnl
      121 gaagfaggaa gskvnsynnn qgyshssgfs ssdgktfgag nkqgssgfqg gagghqagfg
      181 qsgyggaggv htaglgihg
//
LOCUS       XP_077488416             223 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099163 [Amblyomma americanum].
ACCESSION   XP_077488416
VERSION     XP_077488416.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632290.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..223
                     /product="uncharacterized protein LOC144099163"
                     /calculated_mol_wt=20412
     CDS             1..223
                     /gene="LOC144099163"
                     /coded_by="XM_077632290.1:58..729"
                     /db_xref="GeneID:144099163"
ORIGIN      
        1 mkplclavfa aiachvlagk dekvesrggl vggyggghrp gvggfggvgv ggvgikgvpa
       61 gglgyggglg qgglghgglg hgglghvgls qgglghgglg hgglghgglg hsgfgpgglg
      121 hgglvgggag fgqssggfqg gfkkgaaghs hgsggfisgg shsnvqgfkn qqgyssgtgf
      181 sktdsnrygt gfgqhsggfa ggasghkagh gsrgfghvgg vgy
//
LOCUS       XP_077488427             264 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099170 [Amblyomma americanum].
ACCESSION   XP_077488427
VERSION     XP_077488427.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632301.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..264
                     /product="uncharacterized protein LOC144099170"
                     /calculated_mol_wt=24039
     CDS             1..264
                     /gene="LOC144099170"
                     /coded_by="XM_077632301.1:14..808"
                     /db_xref="GeneID:144099170"
ORIGIN      
        1 mktaiillaa lattvlakds dasgkkneta vegrglllgs hnllgacppc pcgygasaag
       61 vagglgqgvg slglgsgagl ahgaatdvhg aginaavgaa ssghglsgvg lahgssgivt
      121 pgagigsgvv gpvagtgpvl apnggvgvva tpavagpgia gglgaghgsa asghsgfqtg
      181 sgfanqaggq nagqgsfayn vggsdsssyg hsssygdsks fgvsasegfn haqsqgshgs
      241 efkkqaagsa asshssgtkt kvvv
//
LOCUS       XP_077488438             386 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099177 [Amblyomma americanum].
ACCESSION   XP_077488438
VERSION     XP_077488438.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632312.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..386
                     /product="uncharacterized protein LOC144099177"
                     /calculated_mol_wt=35120
     CDS             1..386
                     /gene="LOC144099177"
                     /coded_by="XM_077632312.1:42..1202"
                     /db_xref="GeneID:144099177"
ORIGIN      
        1 meiivafvta lalssaavsa edksagkktk iegrgfplss igaigscppc pcnygsgvag
       61 gvrpvagnga glssgagvvp ggaavlsgag lhdtgaggeg lthgahgagf alksghgapv
      121 vaggasirpv vagalpgslr gpglvsgggt svfgapvvtg pgieagagya egasghsnfe
      181 agsgfvrsgg asagvkhlas aglsgvgiha sgsggvgpgi gahgvgsvlk aghggaivtp
      241 gagvhpavgg avlpgaaqgs glvssggvsv ietplvtgsg igaglgagha agasgqssfe
      301 kgsgfankag gnkagqgsfa ynvggsdsss yghsssygds ksfgvsasdg fnhangqgsh
      361 gsefkkeaag saasshssgt ktkvvv
//
LOCUS       XP_077488450             215 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099184 [Amblyomma americanum].
ACCESSION   XP_077488450
VERSION     XP_077488450.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632324.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..215
                     /product="uncharacterized protein LOC144099184"
                     /calculated_mol_wt=19331
     CDS             1..215
                     /gene="LOC144099184"
                     /coded_by="XM_077632324.1:101..748"
                     /db_xref="GeneID:144099184"
ORIGIN      
        1 mkfvifalpa lavlavvtag giigagtglg aaglgagigs gigtgigspv gagfntglig
       61 sslgsavgsg lgigsglgyg gglgglgssg vlglggglgg aglgglayga gfgsyfgssi
      121 gsglsgflsg asgnilgaig sgkgvsyssl rtynnnddyn qaagqlssga knvasdqkqg
      181 ssgfsgstag yntgfgkagy gsaaggsfgg gygrr
//
LOCUS       XP_077488463             122 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099190 [Amblyomma americanum].
ACCESSION   XP_077488463
VERSION     XP_077488463.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632337.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..122
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..122
                     /product="uncharacterized protein LOC144099190"
                     /calculated_mol_wt=13726
     CDS             1..122
                     /gene="LOC144099190"
                     /coded_by="XM_077632337.1:638..1006"
                     /db_xref="GeneID:144099190"
ORIGIN      
        1 mkffylalaf vvllaaaqsg vmgqeeqllm pwdqyrtspl lealrgwirr ptftftrpnf
       61 lpalsanair lrpglqqvlr pmlqrpaiqn mirpalnlrp viatgianlg slirrpflsl
      121 ee
//
LOCUS       XP_077488475             371 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099201 [Amblyomma americanum].
ACCESSION   XP_077488475
VERSION     XP_077488475.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632349.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..371
                     /product="uncharacterized protein LOC144099201"
                     /calculated_mol_wt=39785
     CDS             1..371
                     /gene="LOC144099201"
                     /coded_by="XM_077632349.1:219..1334"
                     /db_xref="GeneID:144099201"
ORIGIN      
        1 mrmipgfvll vllfaqgsrg hqpifhapiv slqpvaaaap awlhgggyah tqilpsgpny
       61 ahqrivthsa pvfhyahppp tivtvhktsa spvlysgwqa pitayakpas iathvhapvf
      121 tavafkhpvt takliqthqh hphvltshhq ayvppvpigr aivtsyfnvp gsstsgapag
      181 fpsldadrap gqsnvlpqsg irftggried ynrrgwirps apayrpqned rledygagsw
      241 arpsgstfkp qaeslfvgpl dnderpamrk hlnlvtpseq vprypvptrl gessswpasl
      301 qdgapqvryg arvargaarr rgggvspgfg drgelrwpfe idfealnkqa kessdqaeta
      361 aadsddngss a
//
LOCUS       XP_077488482             223 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099210 [Amblyomma americanum].
ACCESSION   XP_077488482
VERSION     XP_077488482.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632356.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..223
                     /product="uncharacterized protein LOC144099210"
                     /calculated_mol_wt=25356
     CDS             1..223
                     /gene="LOC144099210"
                     /coded_by="XM_077632356.1:217..888"
                     /db_xref="GeneID:144099210"
ORIGIN      
        1 maslpkeraq sraqdatrkt rqeknhrdsn ldlpalylak gslfgwisdp gsdtssegsa
       61 gdlalgvetv defpyvkisr aemkkqraml anieqmlkfv dknlteksvv mpkvmlnkfl
      121 evehikskmc kmqeenrrlk eevhrrdefi ranglelsga gaglviqqay epfedtsvlq
      181 eqqqnaclpd ssqrqaqdam lrkmlrnark qekkrkhkqr pae
//
LOCUS       XP_077488508             223 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099229 [Amblyomma americanum].
ACCESSION   XP_077488508
VERSION     XP_077488508.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632382.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..223
                     /product="uncharacterized protein LOC144099229"
                     /calculated_mol_wt=25281
     CDS             1..223
                     /gene="LOC144099229"
                     /coded_by="XM_077632382.1:232..903"
                     /db_xref="GeneID:144099229"
ORIGIN      
        1 maslpkeraq sraqdatrkt rqeknhrdsn ldlpalylak gslfgwisdp gsdtssegsa
       61 gdlalgdeiv defpyvkisr aemkkqraml anieqmlkfv aknlteksvv mpkvmlnkfl
      121 evehiksemc kmqeenrrlk eelhrrdefi ranglelsga gaglviqqas epfedtsvlq
      181 eqqenaclpd ssqrqaqdam lrkmlrnark eekkrkhkqr pae
//
LOCUS       XP_077488530             224 aa            linear   INV 25-NOV-2025
DEFINITION  plasmanylethanolamine desaturase 1-like [Amblyomma americanum].
ACCESSION   XP_077488530
VERSION     XP_077488530.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632404.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..224
                     /product="plasmanylethanolamine desaturase 1-like"
                     /calculated_mol_wt=25597
     Region          40..216
                     /region_name="Lipid_desat"
                     /note="Lipid desaturase domain; pfam10520"
                     /db_xref="CDD:463132"
     CDS             1..224
                     /gene="LOC144099239"
                     /coded_by="XM_077632404.1:50..724"
                     /db_xref="GeneID:144099239"
ORIGIN      
        1 mgirrfviel cptivmlgat pfarfrsllt dcarglcgvv tadflsglvh waadtwgsve
       61 lpvigkafir pfrehhidpt sitrhdfiet ngdnfmvpip vlasmawgfa skppqailat
      121 yhwncflfll alfvaftnqi hkwshtyfgl prwvtllqew hvvlprqhhr ihhvaphety
      181 fcittgwlny pleklrfwpa leavisaltg crprsddlqw alkk
//
LOCUS       XP_077488543             108 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099241 [Amblyomma americanum].
ACCESSION   XP_077488543
VERSION     XP_077488543.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632417.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..108
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..108
                     /product="uncharacterized protein LOC144099241"
                     /calculated_mol_wt=12148
     CDS             1..108
                     /gene="LOC144099241"
                     /coded_by="XM_077632417.1:108..434"
                     /db_xref="GeneID:144099241"
ORIGIN      
        1 mvvvlgtsif dcdctcivtl dgakcfegls flaavetlya ayfclnmkyn qevqatlefi
       61 qrqlvgmnpe egsrqlnrri tsvhpkvhsa lqqldklkkk wqdcmsri
//
LOCUS       XP_077488554             108 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099257 [Amblyomma americanum].
ACCESSION   XP_077488554
VERSION     XP_077488554.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632428.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..108
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..108
                     /product="uncharacterized protein LOC144099257"
                     /calculated_mol_wt=12148
     CDS             1..108
                     /gene="LOC144099257"
                     /coded_by="XM_077632428.1:1449..1775"
                     /db_xref="GeneID:144099257"
ORIGIN      
        1 mvvvlgtsif dcdctcivtl dgakcfegls flaavetlya ayfclnmkyn qevqatlefi
       61 qrqlvgmnpe egsrqlnrri tsvhpkvhsa lqqldklkkk wqdcmsri
//
LOCUS       XP_077488569             112 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099264 [Amblyomma americanum].
ACCESSION   XP_077488569
VERSION     XP_077488569.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632443.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..112
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..112
                     /product="uncharacterized protein LOC144099264"
                     /calculated_mol_wt=12435
     CDS             1..112
                     /gene="LOC144099264"
                     /coded_by="XM_077632443.1:2790..3128"
                     /db_xref="GeneID:144099264"
ORIGIN      
        1 mvvvlgtsif dcdctcivtl dgakcfegls flaavetlya ayfclnmkyn qevqatlefi
       61 qrqlvgmnpe egsrqlnrri tsvhpkvhsa lqqldklkkk varlhvsdlv cl
//
LOCUS       XP_077488633             967 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-4-like [Amblyomma americanum].
ACCESSION   XP_077488633
VERSION     XP_077488633.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632507.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..967
                     /product="protein argonaute-4-like"
                     /calculated_mol_wt=108786
     Region          202..271
                     /region_name="ArgoN"
                     /note="N-terminal domain of argonaute; pfam16486"
                     /db_xref="CDD:465134"
     Region          281..332
                     /region_name="ArgoL1"
                     /note="Argonaute linker 1 domain; pfam08699"
                     /db_xref="CDD:462567"
     Region          338..468
                     /region_name="PAZ"
                     /note="PAZ domain; pfam02170"
                     /db_xref="CDD:460472"
     Region          495..924
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(627,631,643..646,649,658,661,665,669)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(696,698,774,914)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..967
                     /gene="LOC144099304"
                     /coded_by="XM_077632507.1:37..2940"
                     /db_xref="GeneID:144099304"
ORIGIN      
        1 metaprsqrr scgtgrptgr spgapsalsf aqavqqrvvr aappqilsst aagaalitra
       61 egtngngaht vaptegvptt stsltaapvq arngvgntks qndelsiqei ertlpshfpr
      121 rpaqgklgrp iqltanhfsv eipsgnvfhy dveicsesrk etnkvpekrk yrcistkinr
      181 lviellvkky rvdlsgcmpa fdgrknlytr relnfrertf tvdfeedqri qnfivkiqya
      241 atvnldalqa vfdrrvsqvp qevlqavdim lrhgpsiklt pigrsffkpp apgeyntlgg
      301 grevwfgyyt svraaqwkpm lnvdisataf yeplpvtdfm cklfsdcrre msagdfknlr
      361 dfqnvrlnke lkglrvkvth lpyprkykvv rvtkepakki yfkmedgtrf svadyfqnry
      421 drlkypnlpc ietgstnhpv ylplevcviv egqhckkkld etqtsemikr taqppakrfn
      481 eirqsvrdlv sssdqclhef gikisteptq lkgrvldpps lvfennyigk pregtwelrg
      541 rhfyksatlt rwtllnlsqf aqrdsldnfv kmlirvgqel gmrieqpldv tttdanrkpv
      601 rnilleeqrk tanlemviiv lakntsyadi kqvaeteigl rtqcvmdnnv ikkcnaalit
      661 nlcqkinakl gginnsllpk ekpkifqkpv iiigadvthp apgdklrpsi aacvgsldai
      721 pykfhasvrv qmadsaatsr leiikdlkdm mkdtlkafyh atrhkperii fyrdgvsegq
      781 fmevrnrevs airlacqels pnetyepalt fivvqkrhht rfmpandrdg vgkcrnmppg
      841 ttvdsvvthp ldfdfylcsh fgiqgtsrps hyyilwddss ltadevqyls dylchtyarc
      901 trsvsipapv yyahlaayra kkhlmskvdv sssssdssgg sadsvstsqy veavkvlesl
      961 ktamyfv
//
LOCUS       XP_077488706             400 aa            linear   INV 25-NOV-2025
DEFINITION  cholesterol 7-desaturase nvd-like [Amblyomma americanum].
ACCESSION   XP_077488706
VERSION     XP_077488706.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632580.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..400
                     /product="cholesterol 7-desaturase nvd-like"
                     /calculated_mol_wt=45141
     Region          57..396
                     /region_name="PobA"
                     /note="Chlorophyllide a oxygenase/letal leaf spot protein
                     [Coenzyme transport and metabolism]; COG5749"
                     /db_xref="CDD:444459"
     CDS             1..400
                     /gene="LOC144099349"
                     /coded_by="XM_077632580.1:47..1249"
                     /db_xref="GeneID:144099349"
ORIGIN      
        1 mawmwpklwt cgfpasvial vcgvrwiivp lrsskksrkq rqqlwdrrfi sessslppvf
       61 tngwipvies selgvdevkp lhvfgedlva frtedgvahv mdaycphlga hlgvmgrvvg
      121 nciecpfhgw rfqadtggca hvpyanktps fvtvktwtcr elcglvfvwf hadgeeaswe
      181 ldgpadvisg emrqmaryet lcsghpqdia qkvadgahln slhratcllp leefsrrpmd
      241 swlcrlmale wtasfsvaae rvstslaiqm silgwkprfa rcrgvvtqvg paltlvsvrn
      301 mfgnfiavvs atpeapfrtr ivhrsygkpg llswlwcqaa ergisnmmdr dvavwsqkaf
      361 ikpvlleedd sirqfrewys qfysrssptw qqvkekslsw
//
LOCUS       XP_077488728             417 aa            linear   INV 25-NOV-2025
DEFINITION  cholesterol 7-desaturase nvd-like [Amblyomma americanum].
ACCESSION   XP_077488728
VERSION     XP_077488728.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632602.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..417
                     /product="cholesterol 7-desaturase nvd-like"
                     /calculated_mol_wt=47133
     Region          72..413
                     /region_name="PobA"
                     /note="Chlorophyllide a oxygenase/letal leaf spot protein
                     [Coenzyme transport and metabolism]; COG5749"
                     /db_xref="CDD:444459"
     CDS             1..417
                     /gene="LOC144099366"
                     /coded_by="XM_077632602.1:26..1279"
                     /db_xref="GeneID:144099366"
ORIGIN      
        1 mdssasfgrn sdrgdrmsrg sassldrfhp faaaapavat vfvvtlfclk rskaakssrk
       61 alhtraaraf rnvppvfpng wipiiesqel qagqvkalsv igqelvafrt edgvahvmda
      121 ycphlgahlg vmgrvvgdci ecpfhgwrfq adtgacahvp yavkvpefvk atkwksreal
      181 glvfvwhhad gadpswelpd veevergpwk qehrfehive thiqdiaeng adighfnqih
      241 rascfltseq fqrtlgnsww grlvnhswqa twtphehtat vdvaacvsvl gtcpeflkhr
      301 vevlqvgpal vlvrmrsrhg dclivqsltp lapfrvrlvh rfypepqlpw iirrlnvigf
      361 rhmverdiav wnhkaylkhp alvkedrmiv afrkwfsqfy sesspkwqdv gekslew
//
LOCUS       XP_077488741             904 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099375 [Amblyomma americanum].
ACCESSION   XP_077488741
VERSION     XP_077488741.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632615.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..904
                     /product="uncharacterized protein LOC144099375"
                     /calculated_mol_wt=101008
     Region          246..518
                     /region_name="S_TKc"
                     /note="Serine/Threonine protein kinases, catalytic domain;
                     smart00220"
                     /db_xref="CDD:214567"
     Site            order(251..254,257,259,272,274,303,319..322,365,369..370,
                     372,382..383)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     Region          679..750
                     /region_name="PDZ"
                     /note="Domain present in PSD-95, Dlg, and ZO-1/2;
                     smart00228"
                     /db_xref="CDD:214570"
     Site            order(679..685,699,702,731,734,737,739)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467153"
     CDS             1..904
                     /gene="LOC144099375"
                     /coded_by="XM_077632615.1:150..2864"
                     /db_xref="GeneID:144099375"
ORIGIN      
        1 mpgsdkfggt serlsnrgdg attmhmttsa rhrggtstia ttntgsassv srsspqtsts
       61 ssseedeass assegtalsp qkrkemmrqk kcsavkvlvt lfpripkvtt vlleffikre
      121 nkvsllrdpl shfcqeqsvl aaqdclqklh rqfityqdir dmvenlmglh slclkrapit
      181 arslglvirk ltiivgelat slekisevpv tdwmavgdav vtrtdkmrid dqppftdyip
      241 ktrdiepvkl lgaggfgavy karykpanli ctvkliasdr fnrpkqacid kvvasvvrsp
      301 flvkyyvcfa tkdayvtvme yvcgvdlmrv veravflpid qvrvimaqlv lavehlhlkg
      361 flhrdikvsn mlmipggrvk lidfdtnkvc lghftkrvmr gyfrrtpfef rdgesagtip
      421 ymapeilkrr pygracdwws tgvvfyklmt grvpfrgktk kllrdriias plkwpkvseh
      481 phsatttakd mvylllkknp verlgskqyr dikshsffdd fdwnrlrttn slcsvpaiye
      541 lmqisppsdp stnaverstl nlspgattst kkrklikiee ltdmdsalhr plftyassgy
      601 rklssmvkes ddpvsvnesf mgesnmssse idyqkttdae snygftgfsk tdtfsrnrra
      661 tekldlilfr kksfgrfwsf gvnleavtge gganfyives vkrgspaqrs qiligdvvva
      721 vngtklaglp lnivrkimns sgdqlvltvl ssspyrimnt rqdmnnilqs cgrqtltlsl
      781 vragcrkgaw ygfttfeakt wdqsqkafsr vhiiqrakdv rvqtvgqnlf pgdvlthvdd
      841 qpadkmshsm lrqnlakarp eiqitiapls plrrkrpsst rlhetfmsdd svgepssvaa
      901 dind
//
LOCUS       XP_077488790             629 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099399 [Amblyomma americanum].
ACCESSION   XP_077488790
VERSION     XP_077488790.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632664.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..629
                     /product="uncharacterized protein LOC144099399"
                     /calculated_mol_wt=71565
     Region          14..612
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..629
                     /gene="LOC144099399"
                     /coded_by="XM_077632664.1:50..1939"
                     /db_xref="GeneID:144099399"
ORIGIN      
        1 mysdamdfsr hvhadpcysf yeyvcggwte rrfdsvldtl lvkfrrtvte lalrtqapsq
       61 fqnpsqraak aymscinvlh kqqkevgelk ailfnaelrw pqqpptgnva spflylckdw
      121 sllflvgifv erggnyvlsp tgqftkvlei wetalynskh nrlfeilynd hvsdestavt
      181 yaqmssienq tisllrhvpa rdedeevltt dnlweltphl ersalfwyda lknrmgllps
      241 ssvtvtirqa sfvrafldlp rvygesnfml ymgwvvaqlf siytdsdlmt efyygtrdat
      301 etmhrrlcfa mvqrtmgfaf naeyvngvvs agvrtdvatm vghvyaafse mltrpgtvfp
      361 eevqlpaydn gtgavfefvd rssnvyltrv fssytdmspl laktweavnk gfsvvlptdv
      421 spwplevvid krtnrktpvl trqqhypnnd fsimpyafaf pvydlgapls irygalgsev
      481 asamaellft nksswnesas dgildevtcf frkthdswqs isdvewqily rlasvgalwe
      541 aysvastden dylagqarmh adqlffffwc ylqcgepagq hscdgplrhl rafqrtfdcg
      601 knshmvidwr ctffgdrttt tttttpapl
//
LOCUS       XP_077488849             115 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099426 [Amblyomma americanum].
ACCESSION   XP_077488849
VERSION     XP_077488849.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632723.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..115
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..115
                     /product="uncharacterized protein LOC144099426"
                     /calculated_mol_wt=12859
     CDS             1..115
                     /gene="LOC144099426"
                     /coded_by="XM_077632723.1:566..913"
                     /db_xref="GeneID:144099426"
ORIGIN      
        1 mtrnpgstyk glshhqqgsa vrasafltdp eyaetrfeta gpeesgslsa sapqlalkmt
       61 lqctprtsai yhkflsmwph qptpavmwsm asmptvlqqn srtlhhrwer lkphw
//
LOCUS       XP_077488884             112 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099451 [Amblyomma americanum].
ACCESSION   XP_077488884
VERSION     XP_077488884.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632758.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..112
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..112
                     /product="uncharacterized protein LOC144099451"
                     /calculated_mol_wt=12121
     CDS             1..112
                     /gene="LOC144099451"
                     /coded_by="XM_077632758.1:109..447"
                     /db_xref="GeneID:144099451"
ORIGIN      
        1 maeqrylayl keeeklakev lqklkdlssk lkaeemmikk alqeekmrvs sagqandstl
       61 vasavaetqa tpctegqsga eeafnsrsqa prrevaspkp hggpnqgmtk ie
//
LOCUS       XP_077488905             219 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099461 [Amblyomma americanum].
ACCESSION   XP_077488905
VERSION     XP_077488905.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632779.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..219
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..219
                     /product="uncharacterized protein LOC144099461"
                     /calculated_mol_wt=23946
     CDS             1..219
                     /gene="LOC144099461"
                     /coded_by="XM_077632779.1:382..1041"
                     /db_xref="GeneID:144099461"
ORIGIN      
        1 mqrpqplaml htghvragtf nrnvacianl rpsfaflvcr shlqegtkat savvrclwel
       61 lvinaefelf kkelrraayd dccddsqddl lseffrtdpq plamaahdgt ewppqpqqlp
      121 hpvpqpqqqp qpqqqpqsqp qqqpqpepqq qpksteaagt rssasgsccl sgiiegaigc
      181 prhplvlgrs sgfppcgrtp avqllrrsdv lqrpvggah
//
LOCUS       XP_077488918             295 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099465 [Amblyomma americanum].
ACCESSION   XP_077488918
VERSION     XP_077488918.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632792.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..295
                     /product="uncharacterized protein LOC144099465"
                     /calculated_mol_wt=32737
     CDS             1..295
                     /gene="LOC144099465"
                     /coded_by="XM_077632792.1:193..1080"
                     /db_xref="GeneID:144099465"
ORIGIN      
        1 mqgpqplaml htghvragtf nrnvaciadl wpsfaflvyr shlqedtkpa savvrclwel
       61 lvinaelelf kkelrraayd dccddshgdl lseyffrtdp ralamaahdg tewppqpeql
      121 phpvpqpqqq pqsqpqqqph pepqpqpqsq pqqqpqsqpq qptqpqlqqq pqsqpqqptq
      181 pqqqpqqqpq qqphpkpqqq pqrqqqlhpe pqeqaqqpns qpqqqpqpks teaagpsssa
      241 sascslngii egaigcprrp lllgrlsgfp pcgrtpavql qlrsdvlqct vggah
//
LOCUS       XP_077488930             131 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099470 [Amblyomma americanum].
ACCESSION   XP_077488930
VERSION     XP_077488930.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632804.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..131
                     /product="uncharacterized protein LOC144099470"
                     /calculated_mol_wt=14633
     CDS             1..131
                     /gene="LOC144099470"
                     /coded_by="XM_077632804.1:179..574"
                     /db_xref="GeneID:144099470"
ORIGIN      
        1 mqgpqplaml htghvragtf nrnvaciadl wpsfaflvcr shlqegtkat svvvrslrel
       61 lvlnaefvff nkeltdaadd hccegshddf lseyfytyrr plamaalggt lwpqpqqepq
      121 pqpphqeqpq p
//
LOCUS       XP_077488938             447 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099478 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077488938
VERSION     XP_077488938.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632812.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..447
                     /product="uncharacterized protein LOC144099478 isoform X1"
                     /calculated_mol_wt=49356
     CDS             1..447
                     /gene="LOC144099478"
                     /coded_by="XM_077632812.1:226..1569"
                     /db_xref="GeneID:144099478"
ORIGIN      
        1 msgqedanaa rentsgelaq pplakpdgnh dpdtgdsrav agqqpdtsta svprstsaep
       61 agprsdsred gtrlaprprp ttadastqte qevkeysatc icregkrlad glreinrnia
      121 asveravdvv sgvstappfq avatetgagi ienaaqdqet ikkeygchkd wrnhprcqgd
      181 eddssqlsfg drrrhnhepm dtnprnpard pmfevsydtp halaatssts ifedaplapp
      241 ssdptshpdd qsalpqrqva dnacaaadpi peapdgqeed ggdpradsln yavsdefryi
      301 yeniearwee vsnhlradln arydrfllec errvesqpga cdrdrireel vvslqrfide
      361 tkdqfrsaci nfdewrvayl esevpstgsa lrqgdqfrvq aavfryevld slpdldrrtr
      421 araaelwkel veqtdldvea ifaelse
//
LOCUS       XP_077488946             340 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099478 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077488946
VERSION     XP_077488946.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632820.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..340
                     /product="uncharacterized protein LOC144099478 isoform X2"
                     /calculated_mol_wt=36708
     CDS             1..340
                     /gene="LOC144099478"
                     /coded_by="XM_077632820.1:226..1248"
                     /db_xref="GeneID:144099478"
ORIGIN      
        1 msgqedanaa rentsgelaq pplakpdgnh dpdtgdsrav agqqpdtsta svprstsaep
       61 agprsdsred gtrlaprprp ttadastqte qevkeysatc icregkrlad glreinrnia
      121 asveravdvv sgvstappfq avatetgagi ienaaqdqet ikkeygchkd wrnhprcqgd
      181 eddssqlsfg drrrhnhepm dtnprnpard pmfevsydtp halaatssts ifedaplapp
      241 ssdptshpdd qsalpqrqva dnacaaadpi peapdgqeed ggdpradsln yavsdefryi
      301 yeniearwee vsnhlradln arrenntkqg rktgrsatqn
//
LOCUS       XP_077488958             115 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099488 [Amblyomma americanum].
ACCESSION   XP_077488958
VERSION     XP_077488958.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632832.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..115
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..115
                     /product="uncharacterized protein LOC144099488"
                     /calculated_mol_wt=12416
     CDS             1..115
                     /gene="LOC144099488"
                     /coded_by="XM_077632832.1:225..572"
                     /db_xref="GeneID:144099488"
ORIGIN      
        1 mlktrhafae tlnrnlasia glrhstvfli craytdvyts atsagvrclr empflsaefa
       61 rfnveltraa yaeggygshd dnhgesirth prpsatttsg gtqckrpppd saeae
//
LOCUS       XP_077488968             104 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099492 [Amblyomma americanum].
ACCESSION   XP_077488968
VERSION     XP_077488968.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632842.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..104
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..104
                     /product="uncharacterized protein LOC144099492"
                     /calculated_mol_wt=11063
     CDS             1..104
                     /gene="LOC144099492"
                     /coded_by="XM_077632842.1:138..452"
                     /db_xref="GeneID:144099492"
ORIGIN      
        1 mglggvavat vvsnactlqn apsrrrlecl vasakcdasr qaslcgglqp qrhpfrppda
       61 farvprlprh nrrghddyvr svrgkrhlsk llipatgvdt sggq
//
LOCUS       XP_077489007             179 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099517 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077489007
VERSION     XP_077489007.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632881.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..179
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..179
                     /product="uncharacterized protein LOC144099517 isoform X1"
                     /calculated_mol_wt=19368
     CDS             1..179
                     /gene="LOC144099517"
                     /coded_by="XM_077632881.1:210..749"
                     /db_xref="GeneID:144099517"
ORIGIN      
        1 mlfsrhacpg ifnrnaacia dlrpsiaflv wralfgegts sdsvataalv rylreqlvle
       61 slfvrfkeel mraadadcgd ifqedvlgka lrkeslssft atndhpiars grrrvrkpta
      121 avrprpprva aaapcrkiss svglssrfrp slgqlrcans dakgrgtvgc rmsprsgcc
//
LOCUS       XP_077489015             132 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099517 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077489015
VERSION     XP_077489015.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632889.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..132
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..132
                     /product="uncharacterized protein LOC144099517 isoform X2"
                     /calculated_mol_wt=14694
     CDS             1..132
                     /gene="LOC144099517"
                     /coded_by="XM_077632889.1:210..608"
                     /db_xref="GeneID:144099517"
ORIGIN      
        1 mlfsrhacpg ifnrnaacia dlrpsiaflv wralfgegts sdsvataalv rylreqlvle
       61 slfvrfkeel mraadadcgd ifqedvlgka lrkeslssft atndhpiars grrrvrkpse
      121 hrletaaepd wt
//
LOCUS       XP_077489038             153 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099532 [Amblyomma americanum].
ACCESSION   XP_077489038
VERSION     XP_077489038.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632912.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..153
                     /product="uncharacterized protein LOC144099532"
                     /calculated_mol_wt=16710
     CDS             1..153
                     /gene="LOC144099532"
                     /coded_by="XM_077632912.1:52..513"
                     /db_xref="GeneID:144099532"
ORIGIN      
        1 myelfaslic padalighga aspkfdafrh apvlgglqpq rrsqrppeaf arvlrlprpt
       61 rrghddylgs cigrllelll rplglqssrp rghqmrtsta lraslmtswa spsaalvgsr
      121 rrllsmmasm srfhgsilst prscscvgfc lpw
//
LOCUS       XP_077489059             550 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099544 [Amblyomma americanum].
ACCESSION   XP_077489059
VERSION     XP_077489059.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632933.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..550
                     /product="uncharacterized protein LOC144099544"
                     /calculated_mol_wt=62396
     Region          92..235
                     /region_name="Transposase_21"
                     /note="Transposase family tnp2; cl29371"
                     /db_xref="CDD:475188"
     Region          <455..504
                     /region_name="Ig_like_ice"
                     /note="Ig-like domain; NF012196"
                     /db_xref="CDD:467952"
     CDS             1..550
                     /gene="LOC144099544"
                     /coded_by="XM_077632933.1:92..1744"
                     /db_xref="GeneID:144099544"
ORIGIN      
        1 mgqpreespe lcyrhlstaq elasiaskhn lthacindll dfcrrrgive lpkdartimq
       61 terkanlehg dsfvhfglae giqhavgqgp apenvelhan idglplhrss qtalwpilcr
      121 vinikksepf mvsaycgagk ppclqeylrp fidevrnlsc nglmvkgvhv nvclkavicd
      181 aparsfvkai tghtgyhace rcsqkghyle nrmtfpdlha pqrtdssfrs qedrrhhtgv
      241 spftslnvdm vkcfpteymh lvslgvmrrl lrnwickgha arlsradrsa lndklreaat
      301 afpkyfqrkp rgteeldrwk atefrtflly lgpvllksil pdelykhfly lhvsvrvlas
      361 pqhhrdlnqf ahdllryfvq efgrlygqkq lvynvhtlah laeqclehgt vdefsafpfe
      421 sylgkvkkrl rttnkplaql srrmsevrac tplstgqvgg dvkvgdcflv dncavvivdi
      481 lggnakagil pngreffrvp idssrmdvrr ysalgqetki wpisyiknkv rciklqykrg
      541 hvvfpllhlq
//
LOCUS       XP_077489072             550 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099550 [Amblyomma americanum].
ACCESSION   XP_077489072
VERSION     XP_077489072.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077632946.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..550
                     /product="uncharacterized protein LOC144099550"
                     /calculated_mol_wt=62396
     Region          92..235
                     /region_name="Transposase_21"
                     /note="Transposase family tnp2; cl29371"
                     /db_xref="CDD:475188"
     CDS             1..550
                     /gene="LOC144099550"
                     /coded_by="XM_077632946.1:92..1744"
                     /db_xref="GeneID:144099550"
ORIGIN      
        1 mgqpreespe lcyrhlstaq elasiaskhn lthacindll dfcrrrgive lpkdartimq
       61 terkanlehg dsfvhfglae giqhavgqgp apenvelhan idglplhrss qtalwpilcr
      121 vinikksepf mvsaycgagk ppclqeylrp fidevrnlsc nglmvkgvhv nvclkavicd
      181 aparsfvkai tghtgyhace rcsqkghyle nrmtfpdlha pqrtdssfrs qedrrhhtgv
      241 spftslnvdm vkcfpteymh lvslgvmrrl lrnwickgha arlsradrsa lndklreaat
      301 afpkyfqrkp rgteeldrwk atefrtflly lgpvllksil pdelykhfly lhvsvrvlas
      361 pqhhrdlnqf ahdllryfvq efgrlygqkq lvynvhtlah laeqclehgt vdefsafpfe
      421 sylgkvkkrl rttnkplaql srrmsevrac tplstgqvgg dvkvgdcflv dncavvivdl
      481 lggnakagil pngreffrvp idssrmdvrr ysalgqetki wpisyiknkv rciklqykrg
      541 hvvfpllhlq
//
LOCUS       XP_077489135             414 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099586 [Amblyomma americanum].
ACCESSION   XP_077489135
VERSION     XP_077489135.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633009.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..414
                     /product="uncharacterized protein LOC144099586"
                     /calculated_mol_wt=43906
     Region          <201..>246
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     Region          240..>397
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..414
                     /gene="LOC144099586"
                     /coded_by="XM_077633009.1:1..1245"
                     /db_xref="GeneID:144099586"
ORIGIN      
        1 msshgaaiaa vagrgnrfas dadkdyeivl ptlptgrvvl ntvflhgdvr arpyrvedfr
       61 dvlakagalp dvvalgayqi nhvwavtfst adaakklaat kelevkgrrc ivfdpedhqv
      121 klrlhwmlhg vadedirtaf aafgnvtevt rerwrvagmk ekgsttrtvl lklkpgvkvd
      181 dlphqvrvag elalvvvpgr pmqclrcggt ghvrrdckvp rcskcrrygh seadcvrtya
      241 satghvaasv saelmdvvea eeaasgaddt gkaegvavqp dvgssntdta vaqesgspat
      301 dpsstpvetp kdgasssvhv erkaaseshd ekddsrmnvg vttpakrpha etkadgekaa
      361 epsvgeppak tpqarwaglr prpkvpaekr ggdkappdqe hvlapddtgg dgiv
//
LOCUS       XP_077489136             212 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099651 [Amblyomma americanum].
ACCESSION   XP_077489136
VERSION     XP_077489136.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633010.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..212
                     /product="uncharacterized protein LOC144099651"
                     /calculated_mol_wt=21822
     CDS             1..212
                     /gene="LOC144099651"
                     /coded_by="XM_077633010.1:49..687"
                     /db_xref="GeneID:144099651"
ORIGIN      
        1 mhsaslksap pgpsdgssps sprlssppcc rgaterglgs cpgdcgrnmd sakwspgrpp
       61 lseiyrglav sggpcgskrg gqagdaghcs raprrcprrl hvresaqpvr avsrppihhr
      121 gtftskgtpp taqarlpgir gerrlfwatr lspyaegvvg wtctasvtaa takvlavlqw
      181 gdsggkgqrd lahgdlgcfs pptyvapasg pp
//
LOCUS       XP_077489137             164 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099667 [Amblyomma americanum].
ACCESSION   XP_077489137
VERSION     XP_077489137.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633011.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..164
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..164
                     /product="uncharacterized protein LOC144099667"
                     /calculated_mol_wt=17116
     CDS             1..164
                     /gene="LOC144099667"
                     /coded_by="XM_077633011.1:35..529"
                     /db_xref="GeneID:144099667"
ORIGIN      
        1 mdsakwspgr pplselyrgl avsgglcgsk rggqagdagh csraprrcpr rlhvresaqp
       61 vravsrppih hrgtftskgt pptaqarllg irgelrlfwa trlspyaegv vgwtctasvt
      121 aatakvlavl qwgdsggkgq rdlahgdlgc fspptyvapa sgpp
//
LOCUS       XP_077489139             212 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099683 [Amblyomma americanum].
ACCESSION   XP_077489139
VERSION     XP_077489139.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633013.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..212
                     /product="uncharacterized protein LOC144099683"
                     /calculated_mol_wt=21829
     CDS             1..212
                     /gene="LOC144099683"
                     /coded_by="XM_077633013.1:45..683"
                     /db_xref="GeneID:144099683"
ORIGIN      
        1 mhsaslksap pgpsdgssps sprlpsppcc rgaaerglgs cpgdcgrnmd sakrspgrpp
       61 hselyrglav sgrpsgskrg gqagdaghcc raprrcprrl hvresaqpvr avsrppinhl
      121 gtftskgtpp taqarlpgir gerrlfwatr lspyaegvvg wtctasvtaa takvlavlqw
      181 gdsggkgqrd lahgdlgcfs pptyvapasg pp
//
LOCUS       XP_077489140             164 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099699 [Amblyomma americanum].
ACCESSION   XP_077489140
VERSION     XP_077489140.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633014.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..164
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..164
                     /product="uncharacterized protein LOC144099699"
                     /calculated_mol_wt=17154
     CDS             1..164
                     /gene="LOC144099699"
                     /coded_by="XM_077633014.1:156..650"
                     /db_xref="GeneID:144099699"
ORIGIN      
        1 mdsakrspgr pplselyrgl avsglpcgsk rggqagdagh ccgaprrcpr rlhvresaqp
       61 vravsrppin hrgtftskgt pptaqarlpg irgerrlfwa trlspyaegv vgwtctasvt
      121 aatakvlavl qwgdgggkgq rhrahgdlgc fsppiyvapa fgpp
//
LOCUS       XP_077489141             166 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099715 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077489141
VERSION     XP_077489141.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633015.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..166
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..166
                     /product="uncharacterized protein LOC144099715 isoform X1"
                     /calculated_mol_wt=18215
     CDS             1..166
                     /gene="LOC144099715"
                     /coded_by="XM_077633015.1:292..792"
                     /db_xref="GeneID:144099715"
ORIGIN      
        1 matdaagtgq rpalqnrgst thlppwrfhf eglsdnnrgg vdspsgasha rcapristlr
       61 sgacpaanid sggtrhhdda gfaatrkasv rstviveldf flqslqnllc pcdhdrqret
      121 srswlsnevr raiynqmstc rhwwyrprqg aalmispssv drkgtr
//
LOCUS       XP_077489142             155 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099715 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077489142
VERSION     XP_077489142.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633016.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..155
                     /product="uncharacterized protein LOC144099715 isoform X2"
                     /calculated_mol_wt=16387
     CDS             1..155
                     /gene="LOC144099715"
                     /coded_by="XM_077633016.1:337..804"
                     /db_xref="GeneID:144099715"
ORIGIN      
        1 matdaagtgq rpalqnrgst thlppwrfhf eglsdnnrgg vdspsgasha rcapristlr
       61 sgacpaanid sggtrhhdda gfaatrkasv rstviveldf fskivrpclg hiltgrlvwe
      121 llrilacggp pifaesslpl rprptkgnqp flave
//
LOCUS       XP_077489143             146 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099752 [Amblyomma americanum].
ACCESSION   XP_077489143
VERSION     XP_077489143.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633017.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..146
                     /product="uncharacterized protein LOC144099752"
                     /calculated_mol_wt=15633
     CDS             1..146
                     /gene="LOC144099752"
                     /coded_by="XM_077633017.1:277..717"
                     /db_xref="GeneID:144099752"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrppfa altsvlccqr pasigpcvlp afgcvilpdp
       61 fmlktkscme vvqasklrvi catdleqagk ladgqwysls kasffsktkt tflgswamyl
      121 gtcgqdnagq hasasskkkh etvnkn
//
LOCUS       XP_077489144             164 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099768 [Amblyomma americanum].
ACCESSION   XP_077489144
VERSION     XP_077489144.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633018.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..164
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..164
                     /product="uncharacterized protein LOC144099768"
                     /calculated_mol_wt=17209
     CDS             1..164
                     /gene="LOC144099768"
                     /coded_by="XM_077633018.1:134..628"
                     /db_xref="GeneID:144099768"
ORIGIN      
        1 mdtakrspgr ppllelyrgl avsgrpcgsk rggqaggagh ccraprrclr rlhvqestqp
       61 vravrrlpin hrgaltskat ppttqarlpg irgqrrlfwa trlspyaegv vgwtctasvt
      121 aattkvlavl qwgdsggngq rdsahgdlgc vspptyiapa sgpp
//
LOCUS       XP_077489145             262 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099784 [Amblyomma americanum].
ACCESSION   XP_077489145
VERSION     XP_077489145.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633019.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..262
                     /product="uncharacterized protein LOC144099784"
                     /calculated_mol_wt=29562
     Region          9..56
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          88..248
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..262
                     /gene="LOC144099784"
                     /coded_by="XM_077633019.1:154..942"
                     /db_xref="GeneID:144099784"
ORIGIN      
        1 mlmqakrfcl snedavlltl iklhhnmsfa llgvlfgihr ttaseifkas lrilaeiskh
       61 avfwpskevv vdnltvyfke yssvravldc teisiqrpkd mesqpltysw ykgvytakvl
      121 vcetpgghis yvskayggra sdshitkesk ilelflpsiy svmldkgflt dklclehqit
      181 mvrppflpki kqlmknedek nqsiagarih leraiermkv fkiltgnlgt elfayiddii
      241 eviagivnls kpifcsdkfl vq
//
LOCUS       XP_077489146             262 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099801 [Amblyomma americanum].
ACCESSION   XP_077489146
VERSION     XP_077489146.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633020.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..262
                     /product="uncharacterized protein LOC144099801"
                     /calculated_mol_wt=29621
     Region          9..59
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          89..248
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..262
                     /gene="LOC144099801"
                     /coded_by="XM_077633020.1:58..846"
                     /db_xref="GeneID:144099801"
ORIGIN      
        1 mlmqgkrfcl snedavlltl iklhhnmsfa llgvlfgihr ttaseifkas vrilaeimkn
       61 avfwpskeav vdnlmvyfke yssvravpdc teisiqrpkd mesqpltysw ykgvytakvl
      121 vcetpgghis yvskayggra sdshitkesk ilelflpsiy svmldkgflt dklciehqit
      181 mvrppflrkr kqltknehek nqsiagarih leraiermkv fkiftgnlgt elfayiddii
      241 eviagivnls kpifcsdkvl vq
//
LOCUS       XP_077489147             276 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099833 [Amblyomma americanum].
ACCESSION   XP_077489147
VERSION     XP_077489147.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633021.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..276
                     /product="uncharacterized protein LOC144099833"
                     /calculated_mol_wt=30726
     CDS             1..276
                     /gene="LOC144099833"
                     /coded_by="XM_077633021.1:22..852"
                     /db_xref="GeneID:144099833"
ORIGIN      
        1 mwldevhvss afvrrqlrsl gadgapeatm ddfylllslm lcnlprgrgs klgtdwfqnr
       61 vndlleffpn vsepprvply seeqadaisr fgeqwphtra alcstllktr fqgpmqqvqe
      121 ymsigwrfag mkhvylilef lggrnswvld lipelrarrd rlqaflcayq rlgadgpymk
      181 llnlaeaatt lrkhlglhvt aayalvvled anwlqykgvp rdlyeeavfa kisdikrmgl
      241 gaplkdvpap ptadgdehtv dgaragepaa tpsaap
//
LOCUS       XP_077489148             196 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099876 [Amblyomma americanum].
ACCESSION   XP_077489148
VERSION     XP_077489148.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633022.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..196
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..196
                     /product="uncharacterized protein LOC144099876"
                     /calculated_mol_wt=20699
     CDS             1..196
                     /gene="LOC144099876"
                     /coded_by="XM_077633022.1:65..655"
                     /db_xref="GeneID:144099876"
ORIGIN      
        1 mhsaslktat nsplvgaafa spcrpsspcw rgtaerslds cletsgrnvd saklapgrpp
       61 vselqqshva svrcyvsehg ehgeqgggdg hcqrdprqrp prldarvsar pgravsrpci
      121 hhhmalttta yppspqdalt sergegplsw asrlytsvgs givcsahrlc chhhqgsgra
      181 ptgrtgrkah tghgtr
//
LOCUS       XP_077489149             230 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099924 [Amblyomma americanum].
ACCESSION   XP_077489149
VERSION     XP_077489149.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633023.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..230
                     /product="uncharacterized protein LOC144099924"
                     /calculated_mol_wt=24613
     CDS             1..230
                     /gene="LOC144099924"
                     /coded_by="XM_077633023.1:20..712"
                     /db_xref="GeneID:144099924"
ORIGIN      
        1 mpiilssaas ghaqflfvdr palsfgrsvv clatppnssv lacaaekslg fclessgrnm
       61 dsakrapgwp pvsehrqgrg schgncgryv dsakrppvsq lqqrlvvwvr sydsergeqg
      121 ggdghcqrdp rqrpprlhar vsaqpgravs cssihhhwaf kstayppapq daltgesreg
      181 plpwacrvys svgsvivcsa qrlcchhhqg sgraptgrtg rkahtghgtr
//
LOCUS       XP_077489150             404 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099940 [Amblyomma americanum].
ACCESSION   XP_077489150
VERSION     XP_077489150.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633024.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..404
                     /product="uncharacterized protein LOC144099940"
                     /calculated_mol_wt=44036
     CDS             1..404
                     /gene="LOC144099940"
                     /coded_by="XM_077633024.1:104..1318"
                     /db_xref="GeneID:144099940"
ORIGIN      
        1 mqsaqespeg rqeasspgvv sppaqlqqes wtpsptspgp qqdaslqaeg awgaaqapeq
       61 cypvfyydga vgplhdplie pepprtssse clsfvlgiaf agvllglgll vvhgplathd
      121 itpeddattr slvatphavg rlgiphrmaa prvagggrkr aaqrsrivvp vggsarnevv
      181 yrapvlspkt ktaatearth dpdehkeirt dyescsrhly thctlpkkef fynvdqqscv
      241 saaedtvhvc nrgsnrfasl diclascvkg rqtsdrcyen tiffpcsrqd vlyepwyfdg
      301 kkcvawsfpq gsclsaghrg vfrswdecrr hcvlhpgpec deapppaetc sprqlrhpyf
      361 admqshggar cvntsrrtle sqrcligsnq fssmsacrra cvrs
//
LOCUS       XP_077489157             169 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099979 [Amblyomma americanum].
ACCESSION   XP_077489157
VERSION     XP_077489157.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633031.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..169
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..169
                     /product="uncharacterized protein LOC144099979"
                     /calculated_mol_wt=17937
     CDS             1..169
                     /gene="LOC144099979"
                     /coded_by="XM_077633031.1:127..636"
                     /db_xref="GeneID:144099979"
ORIGIN      
        1 mchpnsysed dcragasqtc rrkastnpha tsslsdralv ahpvpeveri lvqllrrgsq
       61 rsaqaangdr qpqvllrgpp mtrqavypst qqsatvnhpp rehhakmlfe dskpgtqqak
      121 vaaverkkdv tpdpgyeaag tklgaakpaa kkcsvqqpga kvegdcggp
//
LOCUS       XP_077489166             169 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099989 [Amblyomma americanum].
ACCESSION   XP_077489166
VERSION     XP_077489166.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633040.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..169
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..169
                     /product="uncharacterized protein LOC144099989"
                     /calculated_mol_wt=17937
     CDS             1..169
                     /gene="LOC144099989"
                     /coded_by="XM_077633040.1:78..587"
                     /db_xref="GeneID:144099989"
ORIGIN      
        1 mchpnsysed dcragasqtc rrkastnpha tsslsdralv ahpvpeveri lvqllrrgsq
       61 rsaqaangdr qpqvllrgpp mtrqavypst qqsatvnhpp rehhakmlfe dskpgtqqak
      121 vaaverkkdv tpdpgyeaag tklgaakpaa kkcsvqqpga kvegdcggp
//
LOCUS       XP_077489175             169 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144099997 [Amblyomma americanum].
ACCESSION   XP_077489175
VERSION     XP_077489175.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633049.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..169
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..169
                     /product="uncharacterized protein LOC144099997"
                     /calculated_mol_wt=17922
     CDS             1..169
                     /gene="LOC144099997"
                     /coded_by="XM_077633049.1:127..636"
                     /db_xref="GeneID:144099997"
ORIGIN      
        1 mchpnsysed dcragasqtc rrkastnpha tsslsdralv ahpvpeveri lvqllrrgsq
       61 rsaqaangdr qpqvllrgpp mtrqavypst lqsatvnhpp rehhakmlfe dskpgtqqak
      121 vaaverkkdv tpdpgyeaag tklgaakpaa kkcsvqqpga kvegdcggp
//
LOCUS       XP_077489186             282 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100007 [Amblyomma americanum].
ACCESSION   XP_077489186
VERSION     XP_077489186.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633060.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..282
                     /product="uncharacterized protein LOC144100007"
                     /calculated_mol_wt=31457
     Region          15..173
                     /region_name="Metaviral_G"
                     /note="Metaviral_G glycoprotein; pfam09595"
                     /db_xref="CDD:462833"
     CDS             1..282
                     /gene="LOC144100007"
                     /coded_by="XM_077633060.1:76..924"
                     /db_xref="GeneID:144100007"
ORIGIN      
        1 mrpmliafwi vllcalsich enrpgsenke eagdnqdnpi npgrqsgedn sdpkdneepd
       61 dptknngtkp eygqqpedpt knngkkpegr qqpedptknn vtkpedgqqp edptknnktk
      121 pedgqppdds tknnktkped gqppddsttn nktkpddgps ttdtnktqca dtecdqscke
      181 khknrtdlkh vaghcreksc vctqvilcqk ekceeackak hsdkknltse cqeyvcrctw
      241 atkctpevce klckekystk tglqwscsdf ycicswqkda as
//
LOCUS       XP_077489196             135 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100017 [Amblyomma americanum].
ACCESSION   XP_077489196
VERSION     XP_077489196.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633070.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..135
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..135
                     /product="uncharacterized protein LOC144100017"
                     /calculated_mol_wt=14909
     CDS             1..135
                     /gene="LOC144100017"
                     /coded_by="XM_077633070.1:25..432"
                     /db_xref="GeneID:144100017"
ORIGIN      
        1 mamdvyrdaa ahqnlprmha sdnerdlcea gdprplagcd ddgwlkvtpe mvvvvefsvd
       61 yesevsseel esfeagewig ldsqiemedn vdtlsdteti ytewdnhsdl ytdedesdag
      121 slfqeifsgg sdgea
//
LOCUS       XP_077489205             138 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100028 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077489205
VERSION     XP_077489205.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633079.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..138
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..138
                     /product="uncharacterized protein LOC144100028 isoform X1"
                     /calculated_mol_wt=14852
     CDS             1..138
                     /gene="LOC144100028"
                     /coded_by="XM_077633079.1:84..500"
                     /db_xref="GeneID:144100028"
ORIGIN      
        1 megddgrgfy lvdevsadle ldedsalaas vhtdqekqrq apeeegssda sepdkksedk
       61 ddksveklad avqvfldtdp ppaptdsseh dvttmaedgq mayatmmslq qaegdslaqa
      121 aqmpgdqpfk kdedeepk
//
LOCUS       XP_077489213             137 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100028 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077489213
VERSION     XP_077489213.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633087.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..137
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..137
                     /product="uncharacterized protein LOC144100028 isoform X2"
                     /calculated_mol_wt=14781
     CDS             1..137
                     /gene="LOC144100028"
                     /coded_by="XM_077633087.1:84..497"
                     /db_xref="GeneID:144100028"
ORIGIN      
        1 megddgrgfy lvdevsadle ldedsalaas vhtdqekqrq apeeegssda sepdkksedk
       61 ddksveklad avqvfldtdp ppaptdsseh dvttmaedgq mayatmmslq qegdslaqaa
      121 qmpgdqpfkk dedeepk
//
LOCUS       XP_077489221             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100043 [Amblyomma americanum].
ACCESSION   XP_077489221
VERSION     XP_077489221.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633095.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144100043"
                     /calculated_mol_wt=15536
     CDS             1..141
                     /gene="LOC144100043"
                     /coded_by="XM_077633095.1:86..511"
                     /db_xref="GeneID:144100043"
ORIGIN      
        1 megddgrgff lvddvdiead ldedstlppa lpaepaevqr qmqegevacv nnqwekkpes
       61 knekseeqlq egaavpaqte tppvepdtsh hdvatmaddg qmayammmcm dqegdrlpqe
      121 tevagdqptk edkdeekkdq e
//
LOCUS       XP_077489230             306 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100053 [Amblyomma americanum].
ACCESSION   XP_077489230
VERSION     XP_077489230.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633104.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..306
                     /product="uncharacterized protein LOC144100053"
                     /calculated_mol_wt=27591
     CDS             1..306
                     /gene="LOC144100053"
                     /coded_by="XM_077633104.1:55..975"
                     /db_xref="GeneID:144100053"
ORIGIN      
        1 mlfcncaass nwllpaliaf llfpygracs tcaagtqnqg gtsgtgggtt gggvgtpcgg
       61 daqvcgstgv gtgggtggdg sapgaggsga ggsgaggsgt ggsgtggsgt ggsgtggsgt
      121 gcsgtggtgt sgtgpggtgs cgtatggtat ggtgngaagt ggtggtgtng tgtggsgagg
      181 tggigsgtss aggvgtgggs ggsgdtficv lrqfrvpqin dlvtclqnlr qicvqpgmtv
      241 tlgdvlravl qllgclltyv lylwerflgd qlgdvgglla gllfplnapi ralveqlgva
      301 lqqavg
//
LOCUS       XP_077489243             517 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100074 [Amblyomma americanum].
ACCESSION   XP_077489243
VERSION     XP_077489243.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633117.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..517
                     /product="uncharacterized protein LOC144100074"
                     /calculated_mol_wt=56293
     CDS             1..517
                     /gene="LOC144100074"
                     /coded_by="XM_077633117.1:137..1690"
                     /db_xref="GeneID:144100074"
ORIGIN      
        1 mpsypteasn qfliqapfsq shgpqqaafi dirhgvqghs ayvageinnf epvsglsllp
       61 eegegvsvkm sgrgdlwsss rpnrtgcavg gssdasnpti tasessatsk tednsstrsi
      121 ssmaiywvpe eikryfrdcc rntegsslsp pgsctnckek prerefrrfa larntllvvl
      181 avavipllvg mvadvckalw gsgvdrrppm slrkdetqat sgtagypagt vapvrksaps
      241 gtkaedndps iqvtgtgeee mgvsthspfl hapiheaeer lnedgrsspg eevsptkpha
      301 qekmtedtry rpgissanvn nvpeqspcga faftycqspk peffykhavn acvaaatdhv
      361 glcsrgrnrf askrncreec aetkslpehc leravfekcg redvrgdwwh fdghacrawn
      421 ftsgmcpsyr sdvfssqqdc lakcrartgk llcraatpev cysdhlrfpy faladpealc
      481 lrvsdvnhlg rrcltgpnrf eslaacqktc vlnslrv
//
LOCUS       XP_077489255             687 aa            linear   INV 25-NOV-2025
DEFINITION  kell blood group glycoprotein-like [Amblyomma americanum].
ACCESSION   XP_077489255
VERSION     XP_077489255.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633129.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..687
                     /product="kell blood group glycoprotein-like"
                     /calculated_mol_wt=78004
     Region          98..682
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..687
                     /gene="LOC144100083"
                     /coded_by="XM_077633129.1:123..2186"
                     /db_xref="GeneID:144100083"
ORIGIN      
        1 mgiqgtegvr ivsrpadcsl gnwikvvllp vllvfaatgl iymtttywmr iqrgyfwrts
       61 pllhrvknsd ipllpycdsm acktyrsllk ssvnrsqnpc kdfyrfvcdg wrkqhrvlsv
      121 vdaaqdtmyt halneiendp gfamskntaa asvpnvrkri adlvascvqy srnslhelki
      181 flaerhlpwp atsprdllei lldfsgkwnl hlwfqmtldl apyrsgtgep llkiansakf
      241 rawialmram sgqrketqls lryrkyirsv lglfgvsesr aeqlasaiqe mdrltldvlg
      301 pamadpeqki lrmsiqnmaa tatpgiptgr wlqllneyfa warlfsatne vqlenpgllr
      361 avaylrgrka etrealtlsl glrvvtelgw madkeiadgt lqlmdlpwsa htrrclveve
      421 intgvawssl fprhqgvetl vqnvrdilvs dvmrrsnatl qlsarkgreq wdiesylvnl
      481 lpdpnpgpsf fiiwkslmni qwrlqqrgln nvirprsvls htwsiygalt aseeffvfpl
      541 yhpelppavn yggagrllad evlrvlfyep fkgqqqvgqy yyvgdlnegh aaislpewsp
      601 fhvdtralla awkayrqdlq rhstdvhsgr ssrladdrlf fvascyalcs sgyymgeldg
      661 dasrrcnvat ealwefrtaf qcdnqkd
//
LOCUS       XP_077489262             299 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100095 [Amblyomma americanum].
ACCESSION   XP_077489262
VERSION     XP_077489262.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633136.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..299
                     /product="uncharacterized protein LOC144100095"
                     /calculated_mol_wt=33961
     CDS             1..299
                     /gene="LOC144100095"
                     /coded_by="XM_077633136.1:269..1168"
                     /db_xref="GeneID:144100095"
ORIGIN      
        1 mgvspsnvwd phrplplpll klfidvkgnr glhifypfgw mknfettlpt pyfygmtarm
       61 fcfapnvlkq iqilvyeegn eqewnvgldv tsayvyysss egprgkkrvy aaaiapigsy
      121 ysiavqmved rmfkllfndq ekynadmdti sgktwkvrfs vgdhvvcslh ytdekkkffp
      181 anglgpsfwf pglklkvgts vkfqgeavrf neadiieakf ghpdmsiafn sgnwyvgrti
      241 aflvkrtees fliladfane garrispgde pyksgeikpe fsdsfrllnv yvewstfts
//
LOCUS       XP_077489280             540 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100118 [Amblyomma americanum].
ACCESSION   XP_077489280
VERSION     XP_077489280.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633154.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..540
                     /product="uncharacterized protein LOC144100118"
                     /calculated_mol_wt=59254
     CDS             1..540
                     /gene="LOC144100118"
                     /coded_by="XM_077633154.1:146..1768"
                     /db_xref="GeneID:144100118"
ORIGIN      
        1 mdsryknnyq pqsmntpqqp ppempsypte asnqfliqap fsqshgpqqa afidirhgvq
       61 ghsayvagel nnfepvsgls llpeegegvs vkmsgrgdlw sssrpnrtgc avggsshasn
      121 ptitasessa tsktednsst rsissmaiyw vpeeikryfr dccrntegss lsppeswtnc
      181 kekprerefr rfalarntll vvlavavipl lvgmvadvck alwgsgvdrr ppmslrkdea
      241 qatrgaagyp agtvapvrks apsrtkaedn dppiqvtgtg eeemgvsths pflhapihea
      301 eerlnehgms spgeevsptk phaqekmted sryrpgissa nvnnvpeqsp cgafaftycq
      361 spkpeffykh avnacvaaat dhvglcsrgr nrfaskrncr eecfetkslp khcleravfe
      421 kcgredvrgd wwhfdghacr awnftsgmcp syrsdvfssq qdclakcgar tgkvlcraat
      481 pevcysdhlr fpyfaladpe alclrvsdvn hlgrrcltgp nrfeslaacq dtcvlnslrv
//
LOCUS       XP_077489326             414 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100175 [Amblyomma americanum].
ACCESSION   XP_077489326
VERSION     XP_077489326.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633200.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..414
                     /product="uncharacterized protein LOC144100175"
                     /calculated_mol_wt=43906
     Region          <201..>246
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     Region          240..>397
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..414
                     /gene="LOC144100175"
                     /coded_by="XM_077633200.1:33..1277"
                     /db_xref="GeneID:144100175"
ORIGIN      
        1 msshgaaiaa vagrgnrfas dadkdyeivl ptlptgrvvl ntvflhgdvr arpyrvedfr
       61 dvlakagalp dvvalgayqi nhvwavtfst adaakklaat kelevkgrrc ivfdpedhqv
      121 klrlhwmlhg vadedirtaf aafgnvtevt rerwrvagmk ekgsttrtvl lklkpgvkvd
      181 dlphqvrvag elalvvvpgr pmqclrcggt ghvrrdckvp rcskcrrygh seadcvrtya
      241 satghvaasv saelmdvvea eeaasgaddt gkaegvavqp dvgssntdta vaqesgspat
      301 dpsstpvetp kdgasssvhv erkaaseshd ekddsrmnvg vttpakrpha etkadgekaa
      361 epsvgeppak tpqarwaglr prpkvpaekr ggdkappdqe hvlapddtgg dgiv
//
LOCUS       XP_077489336             198 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100192 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077489336
VERSION     XP_077489336.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633210.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..198
                     /product="uncharacterized protein LOC144100192 isoform X1"
                     /calculated_mol_wt=21817
     CDS             1..198
                     /gene="LOC144100192"
                     /coded_by="XM_077633210.1:322..918"
                     /db_xref="GeneID:144100192"
ORIGIN      
        1 mrfsrhacaw ifnrnaacia dlrpsisflv wralfgkdtr gdsvataviv rylreqvvle
       61 aqfarfkeel mraadadcgd ifqedvlgka lrkesrssst aandhpiars grrrvrkpta
      121 aagrprppra avaapyrris ssvgfssrfr pslggttplr kfsgdgawyc glsdeptfrl
      181 llmsvactgq lplfkhvt
//
LOCUS       XP_077489343             198 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100192 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077489343
VERSION     XP_077489343.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633217.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..198
                     /product="uncharacterized protein LOC144100192 isoform X1"
                     /calculated_mol_wt=21817
     CDS             1..198
                     /gene="LOC144100192"
                     /coded_by="XM_077633217.1:273..869"
                     /db_xref="GeneID:144100192"
ORIGIN      
        1 mrfsrhacaw ifnrnaacia dlrpsisflv wralfgkdtr gdsvataviv rylreqvvle
       61 aqfarfkeel mraadadcgd ifqedvlgka lrkesrssst aandhpiars grrrvrkpta
      121 aagrprppra avaapyrris ssvgfssrfr pslggttplr kfsgdgawyc glsdeptfrl
      181 llmsvactgq lplfkhvt
//
LOCUS       XP_077489349             198 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100192 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077489349
VERSION     XP_077489349.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633223.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..198
                     /product="uncharacterized protein LOC144100192 isoform X1"
                     /calculated_mol_wt=21817
     CDS             1..198
                     /gene="LOC144100192"
                     /coded_by="XM_077633223.1:270..866"
                     /db_xref="GeneID:144100192"
ORIGIN      
        1 mrfsrhacaw ifnrnaacia dlrpsisflv wralfgkdtr gdsvataviv rylreqvvle
       61 aqfarfkeel mraadadcgd ifqedvlgka lrkesrssst aandhpiars grrrvrkpta
      121 aagrprppra avaapyrris ssvgfssrfr pslggttplr kfsgdgawyc glsdeptfrl
      181 llmsvactgq lplfkhvt
//
LOCUS       XP_077489357             198 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100192 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077489357
VERSION     XP_077489357.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633231.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..198
                     /product="uncharacterized protein LOC144100192 isoform X1"
                     /calculated_mol_wt=21817
     CDS             1..198
                     /gene="LOC144100192"
                     /coded_by="XM_077633231.1:201..797"
                     /db_xref="GeneID:144100192"
ORIGIN      
        1 mrfsrhacaw ifnrnaacia dlrpsisflv wralfgkdtr gdsvataviv rylreqvvle
       61 aqfarfkeel mraadadcgd ifqedvlgka lrkesrssst aandhpiars grrrvrkpta
      121 aagrprppra avaapyrris ssvgfssrfr pslggttplr kfsgdgawyc glsdeptfrl
      181 llmsvactgq lplfkhvt
//
LOCUS       XP_077489363             197 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100192 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077489363
VERSION     XP_077489363.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633237.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..197
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..197
                     /product="uncharacterized protein LOC144100192 isoform X2"
                     /calculated_mol_wt=21746
     CDS             1..197
                     /gene="LOC144100192"
                     /coded_by="XM_077633237.1:322..915"
                     /db_xref="GeneID:144100192"
ORIGIN      
        1 mrfsrhacaw ifnrnaacia dlrpsisflv wralfgkdtr gdsvataviv rylreqvvle
       61 aqfarfkeel mraadadcgd ifqedvlgka lrkesrssst aandhpiars grrrvrkpta
      121 agrprppraa vaapyrriss svgfssrfrp slggttplrk fsgdgawycg lsdeptfrll
      181 lmsvactgql plfkhvt
//
LOCUS       XP_077489394             790 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme-like 1 [Amblyomma americanum].
ACCESSION   XP_077489394
VERSION     XP_077489394.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633268.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..790
                     /product="endothelin-converting enzyme-like 1"
                     /calculated_mol_wt=88682
     Region          201..785
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..790
                     /gene="LOC144100264"
                     /coded_by="XM_077633268.1:105..2477"
                     /db_xref="GeneID:144100264"
ORIGIN      
        1 mpkrpavplg fssvsiaddl psashsgptt tsadsdleaf wggrslvsrp rrpgsirrsl
       61 rvvvalneme qplqpaaleg argdapvpap vyaqragdhr rqlpahqgtg gvrivsrpak
      121 cslgnwikvv llpvllvfaa taliymttty wmriqrghfw rtspllhsvk ntdppllpsc
      181 dstackmyrs qlksslnksq npckdfyrfv cdgwrqqhrv lsvvdaaqdm mythalndie
      241 gdpgfatskn saaasvpnvr krvadlvasc vqysrnslhe lkmflaerhl pwpatsprdl
      301 leilldfsgk wnlhlwfqvt ldlapyrsgt gepllkigns akfrawialm ramsgqrket
      361 qlslryrkyi rsvlglfgvs esraeqlasa iqemdrltld vlgpamadpe qkisrmsiqn
      421 mtatatpgip igrwlpllnk yfawarpfsa nnevqlenpg llravaylrg rkaetrealt
      481 lslglrvvte lgwmadkeia dgtlqlmdlp wsahtrrclv evemntgvaw sslfprhqgv
      541 etlvqnvrdi lvsdemrrsn atlqlsarng rdqwdiesyl vnvlpeanpg ssffiiwksl
      601 mniqwrlqqr dlnnvirprs vlshtwsiyg altaseeffv fplyhpelpp avnyggagrl
      661 ladevlrvlf yepfhgqqqv gqyyyvgnln egdaaqslpe wspfhvdtra lltawkayrl
      721 dlqrhftdvy sgrssrladd rlffvascya lcssgyymge ldgdasrrcn vatealwefr
      781 mafqcdsqkg
//
LOCUS       XP_077489395             403 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100275 [Amblyomma americanum].
ACCESSION   XP_077489395
VERSION     XP_077489395.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633269.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..403
                     /product="uncharacterized protein LOC144100275"
                     /calculated_mol_wt=43971
     CDS             1..403
                     /gene="LOC144100275"
                     /coded_by="XM_077633269.1:89..1300"
                     /db_xref="GeneID:144100275"
ORIGIN      
        1 mqsaqespeg rqeadspgvv sppahlqqes gppsptspgp qqdasvqaea awgaaqapeq
       61 cypvfydgam dplhdsliep eppktsshec lsfvlgiafa gmllglglla vhgpfathdi
      121 tpedgarars laatphavgr pgiphrmaap raagggrkra aqrsrfvlpf ggrarnevvy
      181 rapvlstktt tavsearthd pdedqeirtd sescnrhlyt hctlpkkeff ynvdqqscvs
      241 aaedtvhvcn rgsnrfasld iclascvkgr qtsdrcyest iffpcsrqdv lyepwyfdgk
      301 rcvawsfpqg sclsashrgv frswdecrrh cvlhpgpecd eapppaetcs lrqlrhpyfa
      361 dmqshggarc vnasrrmles qrcligsnrf ssmsacrrac vrs
//
LOCUS       XP_077489404             212 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100284 [Amblyomma americanum].
ACCESSION   XP_077489404
VERSION     XP_077489404.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633278.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..212
                     /product="uncharacterized protein LOC144100284"
                     /calculated_mol_wt=21711
     CDS             1..212
                     /gene="LOC144100284"
                     /coded_by="XM_077633278.1:49..687"
                     /db_xref="GeneID:144100284"
ORIGIN      
        1 mhsaslksap pgpsdgssps sprlssppcc pgaterglgs cpgdcgrnid sarwspgrpp
       61 lselyrglav sggpcgskrg gqagdaghcs raphrcprrl hvresaqpvr avsrppihhr
      121 gtftskgtpp taqallpgir gerrlfwatr lspyaegvvg wtctasvtaa takvlavlqw
      181 gdsggkgqrd lahgdlgcfs pptyvapasg pp
//
LOCUS       XP_077489409             212 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100295 [Amblyomma americanum].
ACCESSION   XP_077489409
VERSION     XP_077489409.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633283.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..212
                     /product="uncharacterized protein LOC144100295"
                     /calculated_mol_wt=21754
     CDS             1..212
                     /gene="LOC144100295"
                     /coded_by="XM_077633283.1:80..718"
                     /db_xref="GeneID:144100295"
ORIGIN      
        1 mhsaslksap pgpsdgssps sprlssppcc pgaterglgs cpgdcgrnid sarwspgrpp
       61 lselyrglav sggpcgskrg gqagdaghcs raphrcprrl hvresaqpvr avsrppihhr
      121 gtftskgtpp taqarlpgir gerrlfwatr lspyaegvvg wtctasvtaa takvlavlqw
      181 gdsggkgqrd lahgdlgcfs pptyvapasg pp
//
LOCUS       XP_077489418             212 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100305 [Amblyomma americanum].
ACCESSION   XP_077489418
VERSION     XP_077489418.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633292.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..212
                     /product="uncharacterized protein LOC144100305"
                     /calculated_mol_wt=21865
     CDS             1..212
                     /gene="LOC144100305"
                     /coded_by="XM_077633292.1:68..706"
                     /db_xref="GeneID:144100305"
ORIGIN      
        1 mhsaslksap pgpsdgssrs sprlssppcc rgaterglgs cpgdcgrnmd sakwapgrpp
       61 lselyrglav sggpcgskrg gqagdaghcs raprrcprrl hvresaqpvr avsrppihhr
      121 gtftskgtpp taqarlpgir gerrlfwatr lspyaegvvg wtctasvtaa takvlavlqw
      181 gdsggkgqrd lahgdlgcfs pptyvapasg pp
//
LOCUS       XP_077489426             212 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100315 [Amblyomma americanum].
ACCESSION   XP_077489426
VERSION     XP_077489426.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633300.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..212
                     /product="uncharacterized protein LOC144100315"
                     /calculated_mol_wt=21792
     CDS             1..212
                     /gene="LOC144100315"
                     /coded_by="XM_077633300.1:68..706"
                     /db_xref="GeneID:144100315"
ORIGIN      
        1 mhsaslksap pgpsdgssps sprlssppcc rgaaerglgs cpgdcgrnmd sakwspgrpp
       61 lselyrglav sggpcgskrg gqagdaghcs raprrcprrl hvresaqpvr avsrppihhr
      121 gtftskgtpp taqarlpgir gerrlfwatr lspyaegvvg wtctasvtaa takvlavlqw
      181 gdsggkgqrd lahgdlgcfs pptyvapasg pp
//
LOCUS       XP_077489431             212 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100327 [Amblyomma americanum].
ACCESSION   XP_077489431
VERSION     XP_077489431.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633305.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..212
                     /product="uncharacterized protein LOC144100327"
                     /calculated_mol_wt=21711
     CDS             1..212
                     /gene="LOC144100327"
                     /coded_by="XM_077633305.1:2..640"
                     /db_xref="GeneID:144100327"
ORIGIN      
        1 mhsaslksap pgpsdgssps sprlssppcc pgaterglgs cpgdcgrnid sarwspgrpp
       61 lselyrglav sggpcgskrg gqagdaghcs raphrcprrl hvresaqpvr avsrppihhr
      121 gtftskgtpp taqallpgir gerrlfwatr lspyaegvvg wtctasvtaa takvlavlqw
      181 gdsggkgqrd lahgdlgcfs pptyvapasg pp
//
LOCUS       XP_077489448             212 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100345 [Amblyomma americanum].
ACCESSION   XP_077489448
VERSION     XP_077489448.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633322.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..212
                     /product="uncharacterized protein LOC144100345"
                     /calculated_mol_wt=22093
     CDS             1..212
                     /gene="LOC144100345"
                     /coded_by="XM_077633322.1:45..683"
                     /db_xref="GeneID:144100345"
ORIGIN      
        1 mhsaslksas pgpsdgssps sprlptppcc rgaaerglgs fpgncgrnmd sakrspgrpp
       61 lselyrglav sgrpcgrkrg gqagdaghcc raprrcprrl hvresaqpvr avsrppinhr
      121 gtftskgtpp taqarlpgir gerrlfwatr lspyaegvvg wtctasvtaa takvlavlqw
      181 gdsggkgqrd wahgdlgcfs phtyvapasg pp
//
LOCUS       XP_077489470             570 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100370 [Amblyomma americanum].
ACCESSION   XP_077489470
VERSION     XP_077489470.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633344.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..570
                     /product="uncharacterized protein LOC144100370"
                     /calculated_mol_wt=61471
     Region          <382..419
                     /region_name="Kunitz-type"
                     /note="Kunitz/Bovine pancreatic trypsin inhibitor (BPTI)
                     domain; cl00101"
                     /db_xref="CDD:444694"
     Region          426..485
                     /region_name="Kunitz-type"
                     /note="Kunitz/Bovine pancreatic trypsin inhibitor (BPTI)
                     domain; cl00101"
                     /db_xref="CDD:444694"
     Site            order(435..439,442..444,463,465)
                     /site_type="other"
                     /note="putative serine protease binding site [polypeptide
                     binding]"
                     /db_xref="CDD:438634"
     CDS             1..570
                     /gene="LOC144100370"
                     /coded_by="XM_077633344.1:37..1749"
                     /db_xref="GeneID:144100370"
ORIGIN      
        1 mdsyyrqrlr hpifqnapis pplpplpvqr qgtgilkypf sfydsqkqps gktlrfemdq
       61 paepvpvlpl spmvaqnplv aspegsgtiv ssissvyttg aedeesnagt eagqpaapss
      121 taspaaslev trnsaktsta sgvsshldee festsgsaar tsgtsmtsls iseeikrflr
      181 dvrgassrrr vrapiageea eyrnapphrd swramsrrya lltllsallm lmlagisadv
      241 ygtlyrgikd rtaaapfedt vaalqpeaeg rgmpvvqprw psrptarerd ggidqtdaae
      301 eapeaessae tavptederp pisteiasgg gdetmaepad vqeimegrkp dkdvasaslk
      361 sregsgcdap sftycpnpsh efyyeysiha cvatathrvg vcsrginrfa skricrercv
      421 egsqsseect qtplfvecds edvvdawwyf dgkacqrwnf tsglcpaqgg gsgafsslde
      481 csaacvgrpr grlcrgsrsd tcfsdqlrfp yfaaaaeyge gpprclklss atnlghlclt
      541 ganrfptmka chmtcvsnrt diepwrtvar
//
LOCUS       XP_077489487             576 aa            linear   INV 25-NOV-2025
DEFINITION  acetylcholinesterase-1-like [Amblyomma americanum].
ACCESSION   XP_077489487
VERSION     XP_077489487.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633361.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..576
                     /product="acetylcholinesterase-1-like"
                     /calculated_mol_wt=62955
     Region          14..498
                     /region_name="Abhydrolase"
                     /note="alpha/beta hydrolases; cl21494"
                     /db_xref="CDD:473884"
     Site            order(128..130,209..211,214,360,364..365,398,443,446)
                     /site_type="other"
                     /note="substrate binding pocket [chemical binding]"
                     /db_xref="CDD:238191"
     Site            order(210,333,442)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238191"
     CDS             1..576
                     /gene="LOC144100397"
                     /coded_by="XM_077633361.1:140..1870"
                     /db_xref="GeneID:144100397"
ORIGIN      
        1 mrsngeeeda sagcltvrtn tglvkgtlvq frikgqtyst vafygipfaa pptgehrfae
       61 prcvepwngv fdatykrppc aqmdsvlpkn ytikssntte dclhisiwvp gscvrqargh
      121 avifwlyggs flsggnsydf ydgrfmtglg dvvvaapnyr vsvfgylnsg tedvpgnmgl
      181 qdqllaarwl hanvehfggd reritaagqs agaisismhm vsplmsqygp frrafllsaa
      241 aqtplpqnrg dsarqgfadi aeragcrads vaeslrclrk vetskilgav kssaiwfvpt
      301 sqgpllpssf qdlfrrflps rardvvitnv ategtaffem llpgflgskk kitstllkgt
      361 fshlfagvde alidkmmafl grqydladpd ykgwidvigd vlfrcptvsf gkslavlgkn
      421 vyymqyrpkp sfslfsaeta thgddvtllf gfpflypgla sdqdmensyr lmmhlanfak
      481 ngsvprlpdg spwplfseat gypiveqtqt gyvlgapird ctianifqqh fsgkskresd
      541 vtpmskvikl llemlasips maysvsggyt nvshnh
//
LOCUS       XP_077489493             352 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100406 [Amblyomma americanum].
ACCESSION   XP_077489493
VERSION     XP_077489493.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633367.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..352
                     /product="uncharacterized protein LOC144100406"
                     /calculated_mol_wt=39619
     Region          94..146
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          176..343
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..352
                     /gene="LOC144100406"
                     /coded_by="XM_077633367.1:224..1282"
                     /db_xref="GeneID:144100406"
ORIGIN      
        1 mcdegteplv csladtacqt dlgaadirgl edqisglrqq lqaseeasrs tsfieealhg
       61 cdervafytg lprfvmlhsl fklvkdhish srkhsltqfq emvlflmrmr lgcqyqhlay
      121 rfcvsvstas ricdkwlnvf fhrigplvhw pdkeqvmktm pvafvdkygv kvrvildcfe
      181 vfidrtssyl praetwsyyk hhntvnfllg vspqgvvtfl skayggrasd kfiteecgvr
      241 dlleygdvvl adrgflises vglchatlai paftkgkrql ssediertge ianvrihver
      301 vigmvrnkyt ilsgslpvev lklnkygecq idkiarvccv ltnlcnsvvp fd
//
LOCUS       XP_077489504             123 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100417 [Amblyomma americanum].
ACCESSION   XP_077489504
VERSION     XP_077489504.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633378.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..123
                     /product="uncharacterized protein LOC144100417"
                     /calculated_mol_wt=12660
     CDS             1..123
                     /gene="LOC144100417"
                     /coded_by="XM_077633378.1:85..456"
                     /db_xref="GeneID:144100417"
ORIGIN      
        1 mdensgdkap ppvgrargrs rdrartvvvl pgaqvaptvp qfpaamapla alprepgvlr
       61 patvtlpsmp tldsvaglss sidrmaigqg ahlqqtaama psavvtttlr fvseqhplpd
      121 tkh
//
LOCUS       XP_077489521            1016 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077489521
VERSION     XP_077489521.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633395.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1016
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=112781
     Region          <5..>92
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     Region          155..1015
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          543..972
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(675,679,691..694,697,706,709,713,717)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(744,746,822,962)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..1016
                     /gene="LOC144100435"
                     /coded_by="XM_077633395.1:63..3113"
                     /db_xref="GeneID:144100435"
ORIGIN      
        1 mgkgerkdkk kndsaggppa vpsasggnqa tapapspagt srdlfaqvlq qgaapspqpr
       61 avsrgpapaa vpraqptaqp taqpsayrps ygaaaaagda nvaassprpa saaaavsrsq
      121 paaaalpppp pctppaavte qtpeagdqat pggdvsikel ertlpshfpr rpahgqlgrt
      181 iqllanhfsi eiptgsvyhy dvdissetak etkvpeqkny rclstkinri viellvkkyr
      241 qdlancipvf dghknlytrr qlnfrertft vdldedqrsq kfiikiqyat tvnldalhgv
      301 fqkrvqtvpq evlqaidivl rhspsinlap vgrsffrppg pnehnhlggg revwfgyyas
      361 vrpaqwkpml nvdmsatafy eslplvdfmc rffsdsrrvl tpadlrslrd nqyarlnrel
      421 kglrvkvthl pyprkykvvk itreaakdiy fesegsqisv adyfqsryrr lsypnfpcvq
      481 sgspthpvyi plevcelaeg qhcrkklees qtaemikrta kppakrflei rqsvrdmvst
      541 sdkylrefgv kinpeptqvv grildppslv fennsmckpr dgtwdlrgqr fykamsmtqw
      601 ivlnvsrfaq kdcldnfikm lvrigqglgm riaqplavit fdtnrkpmrt vlteqrkqhp
      661 qlemvvavit katnyaeikq vaetelslrt qcildnnvvq kcnaaliqnl cqkinakmgg
      721 innslllqek pkllhrpviv igadvshpsp gdkirpsiaa cvgsldsvps kfhatirvqi
      781 edskakarve iikdlkdmik elllvfyrtt ghkplgivfy rdgvsegqfl evrnhevsai
      841 rlacaemcpt etyeppltfi vvqkrhhtrf mpandrdgvg kfrnvppgtt vdsvvthpld
      901 fdfflcshfg iqgtsrpahy yivwddsnfs addlqklsfy lchtysrcar svsipapvyy
      961 ahlaafrakh hiaskletsg avsqsseggg davlttaqyv eavkvlqelq asmyfv
//
LOCUS       XP_077489529             196 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100445 [Amblyomma americanum].
ACCESSION   XP_077489529
VERSION     XP_077489529.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633403.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..196
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..196
                     /product="uncharacterized protein LOC144100445"
                     /calculated_mol_wt=21875
     Region          39..>98
                     /region_name="SanA"
                     /note="Uncharacterized periplasmic protein SanA, affects
                     membrane permeability for vancomycin [Cell
                     wall/membrane/envelope biogenesis]; COG2949"
                     /db_xref="CDD:442192"
     CDS             1..196
                     /gene="LOC144100445"
                     /coded_by="XM_077633403.1:216..806"
                     /db_xref="GeneID:144100445"
ORIGIN      
        1 mifttvqecv chtvckprvl gtcgdfmegy flktaqklmr kalstfkktl vaiwaahsyl
       61 apvlslqivs mqtstcvsel isdlpptpmv vvlgtsifdc dctcivtldg akcfeglsfl
      121 aametlyaay fclnmkynqe vqatlefiqr qlvgmnpeeg sqqlnrrits vhpklhsalq
      181 qldklkkkwq dcmsri
//
LOCUS       XP_077489537             967 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-4-like [Amblyomma americanum].
ACCESSION   XP_077489537
VERSION     XP_077489537.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633411.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..967
                     /product="protein argonaute-4-like"
                     /calculated_mol_wt=108766
     Region          202..271
                     /region_name="ArgoN"
                     /note="N-terminal domain of argonaute; pfam16486"
                     /db_xref="CDD:465134"
     Region          281..332
                     /region_name="ArgoL1"
                     /note="Argonaute linker 1 domain; pfam08699"
                     /db_xref="CDD:462567"
     Region          338..468
                     /region_name="PAZ"
                     /note="PAZ domain; pfam02170"
                     /db_xref="CDD:460472"
     Region          495..923
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(627,631,643..646,649,658,661,665,669)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(696,698,774,914)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..967
                     /gene="LOC144100454"
                     /coded_by="XM_077633411.1:30..2933"
                     /db_xref="GeneID:144100454"
ORIGIN      
        1 mestprsqrr scgtgrptgr spgapsalsf aqavqqrvvr aappqilsst aagtavitra
       61 egtngngaht vaptegvptt stsptaapvq arngvgntks qndelsiqei ertlpshfpr
      121 rpaqgklgrp iqltanhfsv eipsgnvfhy dveicsesrk etnkvpekrk yrcistkinr
      181 lviellvkky rvdlsgcmpa fdgrknlytr relnfrertf tvdfeedqri qkfivkiqya
      241 atvnldalqa vfdrrvsqvp qevlqavdiv lrhgpsiklt pvgrsffkpp apgeyntlgg
      301 grevwfgyyt svrpalwkpm lnvdisataf yepipvtdfm cklfsdcrre msagdfknlr
      361 dfqnvrlnke lkglrvkvth lpyprkykvv rvtkepakki yfkmedgtrf svadyfqnry
      421 drlkypnlpc ietgsanhpv ylplevcviv egqhckkkld enqtsemikr taqppakrfn
      481 eirqsvrdlv snsdqclref gikisteptq lkgrvldpps lvfennnigk pregtwelrg
      541 rhfyksatlt rwtllnlsqf aqrdsldnfv kmlirvgqel gmsieqpldv tttdanrkpv
      601 rnilleeqrk tanlemviiv lakntryadi kqvaeteigl rtqcvmdnnv ikkcnaalit
      661 nlcqkinakl gginnsllpk ekpkifqkpv iiigadvthp apgdklrpsi aacvgsldai
      721 pykfhasvrv qmadsaatsr leiikdlkdm mkdtlkafyh atrhkperii fyrdgvsegq
      781 fmevrnrevs airlacqels pyetyepalt fivvqkrhht rfmpandrdg vgkcrnvppg
      841 ttvdsvvthp ldfdfylcsh fgiqgtsrps hyyilwddsn ftaddvqnls yylchtyarc
      901 arsvsipapv yyahlaayra knhvmskvdv sssssdssgg sadsvstsqy veavkvlesl
      961 ktamyfv
//
LOCUS       XP_077489542             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100463 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077489542
VERSION     XP_077489542.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633416.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144100463 isoform X1"
                     /calculated_mol_wt=17594
     Region          1..>60
                     /region_name="SanA"
                     /note="Uncharacterized periplasmic protein SanA, affects
                     membrane permeability for vancomycin [Cell
                     wall/membrane/envelope biogenesis]; COG2949"
                     /db_xref="CDD:442192"
     CDS             1..158
                     /gene="LOC144100463"
                     /coded_by="XM_077633416.1:166..642"
                     /db_xref="GeneID:144100463"
ORIGIN      
        1 mrkalstfkk tlvaiwaahs ylapvlslqi vsmqtstcvs elisdlpptp mvvvlgtsif
       61 dcdctcivtl dgakcfegls flaametlya ayfclnmkyn qevqatlefi qrqlvgmnpe
      121 egsqqlnrri tsvhpklhsa lqqldklkkk wqdcmsri
//
LOCUS       XP_077489547             108 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100463 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077489547
VERSION     XP_077489547.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633421.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..108
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..108
                     /product="uncharacterized protein LOC144100463 isoform X2"
                     /calculated_mol_wt=12166
     CDS             1..108
                     /gene="LOC144100463"
                     /coded_by="XM_077633421.1:546..872"
                     /db_xref="GeneID:144100463"
ORIGIN      
        1 mvvvlgtsif dcdctcivtl dgakcfegls flaametlya ayfclnmkyn qevqatlefi
       61 qrqlvgmnpe egsqqlnrri tsvhpklhsa lqqldklkkk wqdcmsri
//
LOCUS       XP_077489553             123 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100484 [Amblyomma americanum].
ACCESSION   XP_077489553
VERSION     XP_077489553.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633427.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..123
                     /product="uncharacterized protein LOC144100484"
                     /calculated_mol_wt=12706
     CDS             1..123
                     /gene="LOC144100484"
                     /coded_by="XM_077633427.1:65..436"
                     /db_xref="GeneID:144100484"
ORIGIN      
        1 mdensgdkap ppvgrargrs rdrartvvvl pgaqaahtvp qfpaamapla alprepgvlr
       61 patvtlpsmp tldsvaglss sfdrmaigqg ahlqqtaama psavvtttlr fvseqhplpd
      121 tkh
//
LOCUS       XP_077489560             123 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100494 [Amblyomma americanum].
ACCESSION   XP_077489560
VERSION     XP_077489560.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633434.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..123
                     /product="uncharacterized protein LOC144100494"
                     /calculated_mol_wt=12666
     CDS             1..123
                     /gene="LOC144100494"
                     /coded_by="XM_077633434.1:85..456"
                     /db_xref="GeneID:144100494"
ORIGIN      
        1 mdensgdkap ppvgrargrs rdrartvvvl pgaqaaptvp qfpaamapla alprepgvlr
       61 patvtlpsmp tldsvaglss sfdrmaigqg ahlqqtaama psavvtttlr fvseqhplpd
      121 tkh
//
LOCUS       XP_077489586             268 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100534 [Amblyomma americanum].
ACCESSION   XP_077489586
VERSION     XP_077489586.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633460.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..268
                     /product="uncharacterized protein LOC144100534"
                     /calculated_mol_wt=29413
     Region          64..148
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     Region          219..253
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..268
                     /gene="LOC144100534"
                     /coded_by="XM_077633460.1:368..1174"
                     /db_xref="GeneID:144100534"
ORIGIN      
        1 maalhahvry pagarggavv gtavsvttar deedagtrlr spsptttisv pfhrvramas
       61 fneklvqevk khtqlwdqhs rlhkeagyre aawmeiaaal avtveecqtr wrtlrdtylk
      121 rkkrrragan gqwsvlerdl gflddflrpr trrstlnaaa padvevkqeq pgedaetrea
      181 avaaaaappq pwplamrpaa vmpdrlaagg aaaqelssdp eeffclslaa hlkrlvprer
      241 siarmkmlet lhnlefgdni geaalgep
//
LOCUS       XP_077489600             207 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100565 [Amblyomma americanum].
ACCESSION   XP_077489600
VERSION     XP_077489600.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633474.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..207
                     /product="uncharacterized protein LOC144100565"
                     /calculated_mol_wt=23244
     Region          7..91
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     Region          158..192
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..207
                     /gene="LOC144100565"
                     /coded_by="XM_077633474.1:167..790"
                     /db_xref="GeneID:144100565"
ORIGIN      
        1 masfneklvq evkkhtqlwd qysrlhkeag yreaawmeia aalavtveec qtrwrtlrdt
       61 ylkrkkrrra gsngqwnvle rdlgflddfl rprtrrstln aaaladvevk heqpgedaem
      121 reaavaaaaa tplamrpaav mphrlaaaga vaqalssdpe elfclslatr lkrllprerh
      181 marmkmlqtl hdlesgenig efalgdp
//
LOCUS       XP_077489613             258 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100586 [Amblyomma americanum].
ACCESSION   XP_077489613
VERSION     XP_077489613.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633487.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..258
                     /product="uncharacterized protein LOC144100586"
                     /calculated_mol_wt=26704
     CDS             1..258
                     /gene="LOC144100586"
                     /coded_by="XM_077633487.1:890..1666"
                     /db_xref="GeneID:144100586"
ORIGIN      
        1 msrrhlwrdi pkkeatrgtt rsitshrvit skmqssqlaq pllshaaatv slanasrpgr
       61 klssgsiqrr gqraaasaat htaccwytrs trklnralpl larhttpcsa aaapvgttdg
      121 rdvaapgsnw vasraadlrr rscapspgrv lgasrrrpvg rtpscasaee sysagsatrg
      181 agtqphsrpg isvatgpgts sgvgqsarvr airlptpatv rdllvvpsss gpatptlgsa
      241 kappelhlnf qpqsfgss
//
LOCUS       XP_077489620             268 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100599 [Amblyomma americanum].
ACCESSION   XP_077489620
VERSION     XP_077489620.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633494.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..268
                     /product="uncharacterized protein LOC144100599"
                     /calculated_mol_wt=29314
     Region          64..148
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     Region          219..253
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..268
                     /gene="LOC144100599"
                     /coded_by="XM_077633494.1:46..852"
                     /db_xref="GeneID:144100599"
ORIGIN      
        1 maalhahvry pagarggavv gtavsvttar deedagtrlr spsptttisv pfhrvramas
       61 fneklvqevk khtqlwdqhs rlhkeagyre aawmeiaaal avtveecqtr wrtlrdtylk
      121 rkkrrragan gqwsvlerdl gflddflrpr trrstlnaaa padvevkqeq pgedaetrea
      181 avaaaaappq pwplamrpaa vmpdrlaagg aaaqelssdp eeffclslaa hlkrlvpger
      241 siarmkmlet lhnlefgdni geaalgep
//
LOCUS       XP_077489643             199 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100629 [Amblyomma americanum].
ACCESSION   XP_077489643
VERSION     XP_077489643.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633517.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..199
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..199
                     /product="uncharacterized protein LOC144100629"
                     /calculated_mol_wt=22222
     CDS             1..199
                     /gene="LOC144100629"
                     /coded_by="XM_077633517.1:90..689"
                     /db_xref="GeneID:144100629"
ORIGIN      
        1 msssnpeget aalqvdftfk aiseektalc asalanflrd snkkevlyyy vqmeleesrg
       61 nrgakeaafl pllaalfkdd vgqlfrvfee gtsiterlhe lpctptvval gsffqnqcyi
      121 iceqqvlfse ptgfgeatrl aflayyvfnm sypvkaattl eflqreifdi nptkgtkwng
      181 kqkcrtivha kimklaanl
//
LOCUS       XP_077489664             585 aa            linear   INV 25-NOV-2025
DEFINITION  sodium-coupled monocarboxylate transporter 2-like [Amblyomma
            americanum].
ACCESSION   XP_077489664
VERSION     XP_077489664.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633538.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..585
                     /product="sodium-coupled monocarboxylate transporter
                     2-like"
                     /calculated_mol_wt=64270
     Region          5..522
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     Site            order(57,60,343,346..347)
                     /site_type="other"
                     /note="Na binding site [ion binding]"
                     /db_xref="CDD:271383"
     CDS             1..585
                     /gene="LOC144100670"
                     /coded_by="XM_077633538.1:118..1875"
                     /db_xref="GeneID:144100670"
ORIGIN      
        1 mslnveyatf gvlmaanlgl glyfafsrra rkvstadevf lgsrtmgtlp laisvlasmi
       61 saigvigfga hyyaygfhya wslvsapllv pivtlivipv lyklrvtsvf eylrlrygnk
      121 vglgacciyl flsqtigava lfsasvaaat vfnlpiqwtt lgigvagtvy talgglrgvv
      181 wtdcvqaili lmapvtvigk viydsrntev llrpmsdini keyafrmeld ftsdenfwsc
      241 flgllvhnll rtgmdqsvvq rymasrtlke akrtmcigvf lnivymtvig lmamalvywy
      301 rdcdpllaga ikkydeilpy yvkehlfnfp cfsglfltgv vsaatstiss iinsqaavcy
      361 mdvvstfvrl eentashftr glaflfgilm tiyslivphl gsavrvvvim hngasgpfvg
      421 mfllaflfpw anskgvvagt llttalqfwq mfgkiyygiq aplmplttdf cavnsssmem
      481 htspptaysd ddvfplyrfs snwcvlssav ltiviglvvs fltggqelke gheqltsdif
      541 lslwrklgll pryektetsd vnqedddsts dtkttltllg nesfv
//
LOCUS       XP_077489670             289 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100688 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077489670
VERSION     XP_077489670.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633544.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..289
                     /product="uncharacterized protein LOC144100688 isoform X1"
                     /calculated_mol_wt=31912
     Region          177..197
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(177,180,193,197)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(182,184,186,188..189,192..193,196,210,212,216..217,
                     220..221,224,238,240,242,244..245,248..249,252)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          185..>254
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          205..225
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          229..>286
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          233..253
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          261..280
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..289
                     /gene="LOC144100688"
                     /coded_by="XM_077633544.1:138..1007"
                     /db_xref="GeneID:144100688"
ORIGIN      
        1 mprsflvrrc gsyarwraav ralaadevpl arqhvrtsqh vltstkrcsd fkwsaaqsde
       61 evsrspsitp pcqdssgsvp svltdysiqa llglktettr qegdveqniy anlmiaaprg
      121 apasavpfpp sespplthst vllpagyspv arrlapapaa arspqdkahr pgrifeckqc
      181 gkafkrsstl sthllihsdt rpypceycgk rfhqksdmkk htyihtgekp hkckvcgkaf
      241 sqssnliths rkhtgfkpfa cevcgrafqr kvdlrrhket qhqssdaar
//
LOCUS       XP_077489672             274 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100688 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077489672
VERSION     XP_077489672.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633546.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..274
                     /product="uncharacterized protein LOC144100688 isoform X2"
                     /calculated_mol_wt=30124
     Region          162..182
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(162,165,178,182)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(167,169,171,173..174,177..178,181,195,197,201..202,
                     205..206,209,223,225,227,229..230,233..234,237)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          170..>239
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          190..210
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          214..>271
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          218..238
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          246..265
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..274
                     /gene="LOC144100688"
                     /coded_by="XM_077633546.1:138..962"
                     /db_xref="GeneID:144100688"
ORIGIN      
        1 mprsflvrrc gsyarwraav ralaadevpl arqhvrtsqh vltstkrcsd fkwsaaqsde
       61 evsrspsitp pcqdssgsvp svltdysiqa llglktetts aaprgapasa vpfppsespp
      121 lthstvllpa gyspvarrla papaaarspq dkahrpgrif eckqcgkafk rsstlsthll
      181 ihsdtrpypc eycgkrfhqk sdmkkhtyih tgekphkckv cgkafsqssn lithsrkhtg
      241 fkpfacevcg rafqrkvdlr rhketqhqss daar
//
LOCUS       XP_077489677             100 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100705 [Amblyomma americanum].
ACCESSION   XP_077489677
VERSION     XP_077489677.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633551.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..100
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..100
                     /product="uncharacterized protein LOC144100705"
                     /calculated_mol_wt=9844
     CDS             1..100
                     /gene="LOC144100705"
                     /coded_by="XM_077633551.1:121..423"
                     /db_xref="GeneID:144100705"
ORIGIN      
        1 mkptlvivff allatamagl yggfggigyg gyggyggygg yggyggyggy ggygfrgfpv
       61 grvfaygssi khgfggyglg lgyggyggyg yggyghgfyg
//
LOCUS       XP_077489684             102 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100714 [Amblyomma americanum].
ACCESSION   XP_077489684
VERSION     XP_077489684.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633558.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..102
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..102
                     /product="uncharacterized protein LOC144100714"
                     /calculated_mol_wt=9989
     CDS             1..102
                     /gene="LOC144100714"
                     /coded_by="XM_077633558.1:126..434"
                     /db_xref="GeneID:144100714"
ORIGIN      
        1 mrsliaavff alvatafagy fggyggyggg yggyggyggy ggyggyggyg gyggyggygg
       61 yggyggypvg rafayhthin hgyggyggyg lggfgyghgy hg
//
LOCUS       XP_077489696             288 aa            linear   INV 25-NOV-2025
DEFINITION  Na(+)/citrate cotransporter-like [Amblyomma americanum].
ACCESSION   XP_077489696
VERSION     XP_077489696.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633570.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..288
                     /product="Na(+)/citrate cotransporter-like"
                     /calculated_mol_wt=32387
     Region          <111..214
                     /region_name="CitT"
                     /note="Di- and tricarboxylate antiporter [Carbohydrate
                     transport and metabolism]; COG0471"
                     /db_xref="CDD:440239"
     CDS             1..288
                     /gene="LOC144100732"
                     /coded_by="XM_077633570.1:52..918"
                     /db_xref="GeneID:144100732"
ORIGIN      
        1 mqvvaivlhw qfvwmtflyr ydapqtvatr aairmtirkr sselgvvtfa dfvqviligi
       61 wvaiyctlft eatfngteye klemdffvvl allslpwgrp ehptteslrp lvqripwsti
      121 faygstftls fivkstkfvt wlretlmtle nhnrllpqaf ltvcasmmae lvsschtvri
      181 llpvafetav aepcnplyya lpitvaassp vilptarvsi allseltdig plsmllygll
      241 lkaliitcvl isvdtlgylm fdwsalpswm mahhmnishe whpggrlp
//
LOCUS       XP_077489704             233 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100744 [Amblyomma americanum].
ACCESSION   XP_077489704
VERSION     XP_077489704.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633578.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..233
                     /product="uncharacterized protein LOC144100744"
                     /calculated_mol_wt=26039
     Region          <54..180
                     /region_name="TesA"
                     /note="Lysophospholipase L1 or related esterase. Includes
                     spore coat protein LipC/YcsK [Cell cycle control, cell
                     division, chromosome partitioning, Lipid transport and
                     metabolism]; COG2755"
                     /db_xref="CDD:442045"
     CDS             1..233
                     /gene="LOC144100744"
                     /coded_by="XM_077633578.1:36..737"
                     /db_xref="GeneID:144100744"
ORIGIN      
        1 maflrgvlvg dsqlkflcss rlrlspvvrt ctfsfsghda islaeiipet cfrradfvvl
       61 yvggndldrg ddpreiashi kdlvlllqen vasvvlvfkv lprhfdeprk aqfedrrrrq
      121 lnrrlsatlk rlsgvhilnp ehrfldasgk pmlslfaadr yhvardrgid qmskiivaal
      181 vkiygpgias asrarpgevy vvhrcrrcga kghktdhcwa ycsprrhaaa grg
//
LOCUS       XP_077489716             188 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100751 [Amblyomma americanum].
ACCESSION   XP_077489716
VERSION     XP_077489716.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633590.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..188
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..188
                     /product="uncharacterized protein LOC144100751"
                     /calculated_mol_wt=21136
     Region          <9..135
                     /region_name="TesA"
                     /note="Lysophospholipase L1 or related esterase. Includes
                     spore coat protein LipC/YcsK [Cell cycle control, cell
                     division, chromosome partitioning, Lipid transport and
                     metabolism]; COG2755"
                     /db_xref="CDD:442045"
     CDS             1..188
                     /gene="LOC144100751"
                     /coded_by="XM_077633590.1:373..939"
                     /db_xref="GeneID:144100751"
ORIGIN      
        1 miqetcfrha dfvvlyvggn dldrgddpre iashikdlvl llqenvasvv lvlkvlprhf
       61 deprkaqfed rrrrqlnrrl satlkrlsgv hilnpehrfl dasgkpmlsl faadryhvar
      121 drgidqmski ivaalvkiyg pgiasasrar pgevyvvhrc rrcgakghkt dhcwaycspr
      181 rhaaagrg
//
LOCUS       XP_077489737             240 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100761 [Amblyomma americanum].
ACCESSION   XP_077489737
VERSION     XP_077489737.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633611.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..240
                     /product="uncharacterized protein LOC144100761"
                     /calculated_mol_wt=27533
     CDS             1..240
                     /gene="LOC144100761"
                     /coded_by="XM_077633611.1:86..808"
                     /db_xref="GeneID:144100761"
ORIGIN      
        1 meeegcrgyh qarnsqpenq fhcelkerap ipsqaqkssl csygsngfrw wktyheidhq
       61 qdcaldrclv pamsfpcset ldaisasqle ddshryyqtr rsqpmyqfyc elkeratips
      121 hadkgslcsy gfngfrwwkp yheaghpeer awdrclatkf sifcseesng qlvhamqgsl
      181 cqftnettpg aeswlgslqt wtvwlssklr fgsepftptg pflspahnrn pyqmaallrh
//
LOCUS       XP_077489755             447 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100780 [Amblyomma americanum].
ACCESSION   XP_077489755
VERSION     XP_077489755.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633629.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..447
                     /product="uncharacterized protein LOC144100780"
                     /calculated_mol_wt=47022
     CDS             1..447
                     /gene="LOC144100780"
                     /coded_by="XM_077633629.1:93..1436"
                     /db_xref="GeneID:144100780"
ORIGIN      
        1 mpgassgvat glnpnakage lpqecrgsdw ywpptpgnts tmptglnpsa kafdlpqqcp
       61 grdryvstlp gysstaatal ytnakglqlp qkcpvsgwyl stttgttstl atglnpnaqa
      121 felpeqcpgs gqyvstthga sstvatglnp nakafelpeq cpgsgqyvst ahgtsrtvat
      181 glnpnakafe mpqqcpissw ylsttsdrts tvetglnpca kvfelpqecp gsdryvsttp
      241 gtsstvatal nfnykafelp eqrpesdqyl ltmpgtsttv ttalnpdvka felpphcpgs
      301 gcyhstmpgs gitvatglnp nakafelpsq cpgsgrhpst vpgtssvert alnpnakpfe
      361 lprqcaepmh hdaqqlcsss ekkpatltmt tmtaalsgts qkmvaqtpad glppmqpren
      421 litplyafrd pllpnmlskd krqpetp
//
LOCUS       XP_077489768             226 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100787 [Amblyomma americanum].
ACCESSION   XP_077489768
VERSION     XP_077489768.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633642.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..226
                     /product="uncharacterized protein LOC144100787"
                     /calculated_mol_wt=24293
     CDS             1..226
                     /gene="LOC144100787"
                     /coded_by="XM_077633642.1:128..808"
                     /db_xref="GeneID:144100787"
ORIGIN      
        1 mwstgrppvs elrlglvvsv rrcgskhges rgdggrrhrt aspwpprlhk pesgkpggai
       61 srppvdhhga ltakasqaaq vigptlepyw qrpalqktrm glsccnilad dlcglgylaw
      121 ltgvrgartv savrrnfaes aigcashlrn hhcnragrap mrrtartqrp rlesrvsrlc
      181 ccfcfdphnf vaagraspgs esscgclvge ggtfedlvpg lyitsy
//
LOCUS       XP_077489782             226 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100792 [Amblyomma americanum].
ACCESSION   XP_077489782
VERSION     XP_077489782.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633656.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..226
                     /product="uncharacterized protein LOC144100792"
                     /calculated_mol_wt=24247
     CDS             1..226
                     /gene="LOC144100792"
                     /coded_by="XM_077633656.1:10..690"
                     /db_xref="GeneID:144100792"
ORIGIN      
        1 mwstgrppvs elrlglvvsv rrcgskhges rgdggrrhra aspwpprlhk pesgkpggai
       61 srppvdhhga ltakasqaaq vigptlepyw qrpalqktrm gpsccnilad dlcglgylaw
      121 ltgvrgartv savrrnfaes aigcashlrn hhcnragrap mrrtartqrp rlesrvsrlc
      181 ccfcfdphnf vaagraspgs esscgclvge ggtfedlvpg lyitsy
//
LOCUS       XP_077489807             188 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100812 [Amblyomma americanum].
ACCESSION   XP_077489807
VERSION     XP_077489807.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633681.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..188
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..188
                     /product="uncharacterized protein LOC144100812"
                     /calculated_mol_wt=20182
     CDS             1..188
                     /gene="LOC144100812"
                     /coded_by="XM_077633681.1:208..774"
                     /db_xref="GeneID:144100812"
ORIGIN      
        1 mrtrelplcf lhssaysltp akitpgaatr akhkgkplql flgattsasr ppkdsaaeta
       61 tatpavssam actrgalrhf pftatrapnc sllawchrer nrllarklwp qcglcnvltl
      121 patnlgappg phrlaptrrq qtgmtrmglw plpprstsva teaacteecp alrslqpqap
      181 tslhidrf
//
LOCUS       XP_077489815             188 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100812 [Amblyomma americanum].
ACCESSION   XP_077489815
VERSION     XP_077489815.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633689.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..188
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..188
                     /product="uncharacterized protein LOC144100812"
                     /calculated_mol_wt=20182
     CDS             1..188
                     /gene="LOC144100812"
                     /coded_by="XM_077633689.1:208..774"
                     /db_xref="GeneID:144100812"
ORIGIN      
        1 mrtrelplcf lhssaysltp akitpgaatr akhkgkplql flgattsasr ppkdsaaeta
       61 tatpavssam actrgalrhf pftatrapnc sllawchrer nrllarklwp qcglcnvltl
      121 patnlgappg phrlaptrrq qtgmtrmglw plpprstsva teaacteecp alrslqpqap
      181 tslhidrf
//
LOCUS       XP_077489842             283 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100829 [Amblyomma americanum].
ACCESSION   XP_077489842
VERSION     XP_077489842.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633716.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..283
                     /product="uncharacterized protein LOC144100829"
                     /calculated_mol_wt=32091
     CDS             1..283
                     /gene="LOC144100829"
                     /coded_by="XM_077633716.1:752..1603"
                     /db_xref="GeneID:144100829"
ORIGIN      
        1 mgnirksvdn dpnveearkk fgrkgnfqdk sglkkqcrvp rdtlettvee vdgvvaahvd
       61 fmakemkrcn pdiqklkvsm qttlpsrrkw ikinspstee lldkypaltl remihqefme
      121 ickvdfernl lgfinghgqr lfsilktsrt aqkifsevqk aiadsegdak kycfavgvlq
      181 llphlvkeea fylrgpdsyp civmndkpht atnvcatfen mkveaidvis gfatvmeiyw
      241 vmnieyskkn kntfavlehf cglkskplkp vtlraialle kan
//
LOCUS       XP_077489850             283 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100829 [Amblyomma americanum].
ACCESSION   XP_077489850
VERSION     XP_077489850.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633724.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..283
                     /product="uncharacterized protein LOC144100829"
                     /calculated_mol_wt=32091
     CDS             1..283
                     /gene="LOC144100829"
                     /coded_by="XM_077633724.1:667..1518"
                     /db_xref="GeneID:144100829"
ORIGIN      
        1 mgnirksvdn dpnveearkk fgrkgnfqdk sglkkqcrvp rdtlettvee vdgvvaahvd
       61 fmakemkrcn pdiqklkvsm qttlpsrrkw ikinspstee lldkypaltl remihqefme
      121 ickvdfernl lgfinghgqr lfsilktsrt aqkifsevqk aiadsegdak kycfavgvlq
      181 llphlvkeea fylrgpdsyp civmndkpht atnvcatfen mkveaidvis gfatvmeiyw
      241 vmnieyskkn kntfavlehf cglkskplkp vtlraialle kan
//
LOCUS       XP_077489865             234 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100851 [Amblyomma americanum].
ACCESSION   XP_077489865
VERSION     XP_077489865.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633739.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..234
                     /product="uncharacterized protein LOC144100851"
                     /calculated_mol_wt=24697
     CDS             1..234
                     /gene="LOC144100851"
                     /coded_by="XM_077633739.1:17..721"
                     /db_xref="GeneID:144100851"
ORIGIN      
        1 mhsasltvim ngsafatsas ppflltscrr npaeegsgsf hdncdncgnc vacaelsprv
       61 lpvvelrqgq visvpschlk cgeqsngddh gdrapnqwlp klhalesaqp gtaerrphcd
      121 rrgslvtkal ltaawlrsgv rvtkalsatp rksagavpis rktsaermtt attttskely
      181 aedaqatqpt cafrtaphir gdtdaagsam rlqppcchpg aspftgrlll mths
//
LOCUS       XP_077489902             234 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100873 [Amblyomma americanum].
ACCESSION   XP_077489902
VERSION     XP_077489902.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633776.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..234
                     /product="uncharacterized protein LOC144100873"
                     /calculated_mol_wt=24697
     CDS             1..234
                     /gene="LOC144100873"
                     /coded_by="XM_077633776.1:17..721"
                     /db_xref="GeneID:144100873"
ORIGIN      
        1 mhsasltvim ngsafatsas ppflltscrr npaeegsgsf hdncdncgnc vacaelsprv
       61 lpvvelrqgq visvpschlk cgeqsngddh gdrapnqwlp klhalesaqp gtaerrphcd
      121 rrgslvtkal ltaawlrsgv rvtkalsatp rksagavpis rktsaermtt attttskely
      181 aedaqatqpt cafrtaphir gdtdaagsam rlqppcchpg aspftgrlll mths
//
LOCUS       XP_077489939             380 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100897 [Amblyomma americanum].
ACCESSION   XP_077489939
VERSION     XP_077489939.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633813.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..380
                     /product="uncharacterized protein LOC144100897"
                     /calculated_mol_wt=41661
     CDS             1..380
                     /gene="LOC144100897"
                     /coded_by="XM_077633813.1:302..1444"
                     /db_xref="GeneID:144100897"
ORIGIN      
        1 mkgscnvvec frsasalaqg lagvvqcmsa vllrmtqvdg fefgisillg itniplacia
       61 atvlkrrsec hdphqyvrln rlsvalfwhs alfnaicalv lvlmpsmpsa qvalavptrf
      121 hrywlffldy vslasvvvpa alvvlffccl ltltgsplrs vvpsreslay pkeakaeyit
      181 lrpyrwvyhd snrtapgetr vgrkphslis hddvlypppr ytfsrssgsg gtsitraqps
      241 qsrdgvlpgc sgmgrltrgt ssddfgedda ldsfvsvsfd dsaecsatgh nchkealftk
      301 psgikaqrgl strgnegerp pqlrrvaavn rgedslanlt nleliarqwr tsatlrriwr
      361 ssersasrrh anhdhsvprd
//
LOCUS       XP_077489949             122 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100907 [Amblyomma americanum].
ACCESSION   XP_077489949
VERSION     XP_077489949.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633823.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..122
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..122
                     /product="uncharacterized protein LOC144100907"
                     /calculated_mol_wt=13531
     Region          2..>61
                     /region_name="Motor_domain"
                     /note="Myosin and Kinesin motor domain; cl22853"
                     /db_xref="CDD:473979"
     CDS             1..122
                     /gene="LOC144100907"
                     /coded_by="XM_077633823.1:131..499"
                     /db_xref="GeneID:144100907"
ORIGIN      
        1 masqaavyae vsqlvqsald gyhvcffayg qagagktrtm vgdedddlrg iipralrqvf
       61 eapqkqpqwt dsthrntpww sslrrcttgp satslclrlk qtrphrcssr wparvglsqw
      121 pa
//
LOCUS       XP_077489957             410 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100915 [Amblyomma americanum].
ACCESSION   XP_077489957
VERSION     XP_077489957.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633831.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..410
                     /product="uncharacterized protein LOC144100915"
                     /calculated_mol_wt=45830
     Region          161..181
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(161,164,177,181)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          <183..266
                     /region_name="SFP1"
                     /note="Putative transcriptional repressor regulating G2/M
                     transition [Transcription / Cell division and chromosome
                     partitioning]; COG5189"
                     /db_xref="CDD:227516"
     Region          189..209
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          217..237
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(217,220,233,237)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(222,224,226,228..229,232..233,236,250,252,256..257,
                     260..261,264,278,280,282,284..285,288..289,292)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          245..265
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          271..293
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          273..293
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          301..321
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          313..338
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     CDS             1..410
                     /gene="LOC144100915"
                     /coded_by="XM_077633831.1:93..1325"
                     /db_xref="GeneID:144100915"
ORIGIN      
        1 malnnstari dgeqnhvsge teqfwdfcsi pvqyecvvps apppydasfc tsdtdmapll
       61 pptdtaprpa rythrrrdst qhsmlpycfi cgqefekldh lqthmkehap fagdspppvs
      121 qdhaaplpvp pdqelpplpp ppassgsssg rdagrllpyp ctacskrfkq vghlrnhlrl
      181 htgekpyece vcekkftqsg hlmshscmhs nsrpyqckhc lkrftqsghl anhvrmhsge
      241 rpyvctvcsk rfkqcghlsn hsrlhsgerp fqcrvctkrf kqsghlknhm rihtrgqpcs
      301 csvcnkrftn aehlsnhmrm hmgehpfecl aceksftqsg rlahyvgtsk gpsstrkdsg
      361 sqpprkapfi irltpaspqk qretpadphe sqptvntppg paqgveprhl
//
LOCUS       XP_077489971             608 aa            linear   INV 25-NOV-2025
DEFINITION  carnitine O-acetyltransferase-like [Amblyomma americanum].
ACCESSION   XP_077489971
VERSION     XP_077489971.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633845.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..608
                     /product="carnitine O-acetyltransferase-like"
                     /calculated_mol_wt=69113
     Region          20..589
                     /region_name="Carn_acyltransf"
                     /note="Choline/Carnitine o-acyltransferase; pfam00755"
                     /db_xref="CDD:459926"
     CDS             1..608
                     /gene="LOC144100921"
                     /coded_by="XM_077633845.1:146..1972"
                     /db_xref="GeneID:144100921"
ORIGIN      
        1 marvavkgvm lsrqkelpkl pvpplretle rlwrtllpil sdqelqasrk aidkfgeagg
       61 egeylhrmlt yrykrmdnwl dewwlhsayl dvrtplpvhs spaalfprqt fsseedclmf
      121 aakltaavle fkdlvdrqal ppdmarhipl dmtmyfkifs lhrvpkpqrd evvsyadead
      181 pprhivvmhn nhlfemnvyn qhsrkpyker dlypqlvaim nqsrsvgppi giltsddrds
      241 wcaanqrlaa agcnaqslea vqrsilvlcl depggetepw evrsplhmlv gggnaqsagn
      301 rwydkimqii vgidgevgli mehapvdgtv vvpimdycyt ymkktarhrl dpptcpddqp
      361 kklefeltke nlddivsakt rmealardvd vighrfeeyg kdfikscrms pdsfiqmafq
      421 lsyyrlhghs patyesastr mfllgrteai rsqskesdtf creymagkln vaerdallrn
      481 aisthkdyas lamygqgvdr vllglrkvse emkrplpdly kdpgyikgin felstsqvsa
      541 hndilmtfgy svpggygicy snqcnqfrfs ictrhcnket savkfrdald ttlrelgnnl
      601 ivlqkakl
//
LOCUS       XP_077489991             780 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme-like 1 [Amblyomma americanum].
ACCESSION   XP_077489991
VERSION     XP_077489991.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633865.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..780
                     /product="endothelin-converting enzyme-like 1"
                     /calculated_mol_wt=87229
     Region          193..>654
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..780
                     /gene="LOC144100938"
                     /coded_by="XM_077633865.1:101..2443"
                     /db_xref="GeneID:144100938"
ORIGIN      
        1 mwrpadgavr grnaaaagae essccsstss gssnsdpghi sdgkrllyll rrpgfkridf
       61 temsellemr raaqrafaar avttadassq ttelpelglg pqvdsvavrq vgwhmnprfk
      121 kvliatagfc svalfiylvs avwirvryrh lfhatsgtgy iddarlplfs gcesaacrmy
      181 maaietslnr sqdpcknlyr fvcdgwkqdh rmlsvldaae dlmygralnt iewaprdirk
      241 qspahsvasi veekvvelak sciepsessl hrlkmfmadh hlpwpmtsrw dlleilldls
      301 gnwnlhlwfq vtldlrpsrk vtgdpvlkig hsaafrawia smrafagqpr rsaqsvryqq
      361 yvrsmlllfd vtesqaddlv tvieamnrlt lavlgpamae pehrtlrmsi rnltktttpg
      421 iaagrlllll nehfiwaqqf ssndivqven pgllravvyl lglksemrea ltlslglrvv
      481 helgwmadqq iadltlelmg lptsvhsrrc lvqiedsvgv awlslfssnr gfqtlvedvr
      541 dilaeivmrh srsvldvraq gndamwsfla svlpqptpga sfftywlnlm sarwrlqqrn
      601 ltnvlkpgsv lmnrwsvhgp ltvsndysvf plyhpdlppa vnyggagrli adeilrsvfq
      661 dpmygkrsrr rqpspmlhrn eslpaespse wsphhvdaka llaalnayrl gtlrhpsgps
      721 sressraqdr lffvascyal cssgdyvdel ygdasrrcne pvmtlsefta afkcetpnqh
//
LOCUS       XP_077490013             222 aa            linear   INV 25-NOV-2025
DEFINITION  peroxiredoxin-6-like [Amblyomma americanum].
ACCESSION   XP_077490013
VERSION     XP_077490013.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633887.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..222
                     /product="peroxiredoxin-6-like"
                     /calculated_mol_wt=24853
     Region          4..218
                     /region_name="PRX_1cys"
                     /note="Peroxiredoxin (PRX) family, 1-cys PRX subfamily;
                     composed of PRXs containing only one conserved cysteine,
                     which serves as the peroxidatic cysteine. They are
                     homodimeric thiol-specific antioxidant (TSA) proteins that
                     confer a protective role in cells by...; cd03016"
                     /db_xref="CDD:239314"
     Site            order(4,42,45..47,50,82,85,111,126,140..142,144..146,
                     148..153,155,158,162..163,172..173,175..176,213,216)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239314"
     Site            order(16,70..72,74..75,77..78,80..81,93,102..103,113,
                     124..125,188,206)
                     /site_type="other"
                     /note="decamer (pentamer of dimers) interface [polypeptide
                     binding]"
                     /db_xref="CDD:239314"
     Site            order(41,44,128)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:239314"
     CDS             1..222
                     /gene="LOC144100951"
                     /coded_by="XM_077633887.1:1..669"
                     /db_xref="GeneID:144100951"
ORIGIN      
        1 mvnlgdtfpn ftvettmgtl dlheffknsw gilfshpsdf tpvcttelga makiypdfek
       61 hnirvialsc nsleshklwv kdiiafndlr vtdlpfpiis dekrelavql gmldpidkds
      121 egipltcrav fiigpdkklk lsmlypatsg rnfpelfrav aslklteekk vatpanwmpg
      181 epcmvvpsvk deeipllfpk gvtwynvpsg rkylrvtpqp dl
//
LOCUS       XP_077490026             180 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100957 [Amblyomma americanum].
ACCESSION   XP_077490026
VERSION     XP_077490026.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633900.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..180
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..180
                     /product="uncharacterized protein LOC144100957"
                     /calculated_mol_wt=19436
     CDS             1..180
                     /gene="LOC144100957"
                     /coded_by="XM_077633900.1:184..726"
                     /db_xref="GeneID:144100957"
ORIGIN      
        1 mqragvvpyr tdspttsgta atvrtlpcgq lgghrspsss waspsrsdge takadneegv
       61 maaatsldis ghrgcrhrrl aitaepsvvr pasthvlnmd gfsangsgwa aprtwsetss
      121 fcstnihlhr ddspesnscr tdrkrrhhrh sqgaefalar wtgwtrqahp rmrisaglpr
//
LOCUS       XP_077490034             180 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100957 [Amblyomma americanum].
ACCESSION   XP_077490034
VERSION     XP_077490034.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633908.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..180
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..180
                     /product="uncharacterized protein LOC144100957"
                     /calculated_mol_wt=19436
     CDS             1..180
                     /gene="LOC144100957"
                     /coded_by="XM_077633908.1:111..653"
                     /db_xref="GeneID:144100957"
ORIGIN      
        1 mqragvvpyr tdspttsgta atvrtlpcgq lgghrspsss waspsrsdge takadneegv
       61 maaatsldis ghrgcrhrrl aitaepsvvr pasthvlnmd gfsangsgwa aprtwsetss
      121 fcstnihlhr ddspesnscr tdrkrrhhrh sqgaefalar wtgwtrqahp rmrisaglpr
//
LOCUS       XP_077490047             118 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100971 [Amblyomma americanum].
ACCESSION   XP_077490047
VERSION     XP_077490047.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633921.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..118
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..118
                     /product="uncharacterized protein LOC144100971"
                     /calculated_mol_wt=14261
     CDS             1..118
                     /gene="LOC144100971"
                     /coded_by="XM_077633921.1:337..693"
                     /db_xref="GeneID:144100971"
ORIGIN      
        1 medddytsrs arseptrksg krtqaakqeq pdyefieksm rhdefikere fliesrrial
       61 eeerltweke rtlkelqlke nemtqkaqdq aeerrlraee raeerrereg errmgaaq
//
LOCUS       XP_077490062             118 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100976 [Amblyomma americanum].
ACCESSION   XP_077490062
VERSION     XP_077490062.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633936.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..118
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..118
                     /product="uncharacterized protein LOC144100976"
                     /calculated_mol_wt=14261
     CDS             1..118
                     /gene="LOC144100976"
                     /coded_by="XM_077633936.1:517..873"
                     /db_xref="GeneID:144100976"
ORIGIN      
        1 medddytsrs arseptrksg krtqaakqeq pdyefieksm rhdefikere fliesrrial
       61 eeerltweke rtlkelqlke nemtqkaqdq aeerrlraee raeerrereg errmgaaq
//
LOCUS       XP_077490071             161 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100983 [Amblyomma americanum].
ACCESSION   XP_077490071
VERSION     XP_077490071.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633945.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..161
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..161
                     /product="uncharacterized protein LOC144100983"
                     /calculated_mol_wt=18174
     CDS             1..161
                     /gene="LOC144100983"
                     /coded_by="XM_077633945.1:332..817"
                     /db_xref="GeneID:144100983"
ORIGIN      
        1 mrrwaplrav rvpsrregga stgttqltkr qrerlealvs kkapyslapc rhprefpala
       61 dflcfrklhe dyyrrvrrtf gaerpttttr epsahatlgs aksrasakst gssrrgspqq
      121 arvplesrlh mrrwaplrav rvpsrrevlg geahnkpech s
//
LOCUS       XP_077490097             184 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144100998 [Amblyomma americanum].
ACCESSION   XP_077490097
VERSION     XP_077490097.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633971.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..184
                     /product="uncharacterized protein LOC144100998"
                     /calculated_mol_wt=20097
     CDS             1..184
                     /gene="LOC144100998"
                     /coded_by="XM_077633971.1:3..557"
                     /db_xref="GeneID:144100998"
ORIGIN      
        1 mprrpssdnc laflvvalal nsrfctgcye rrdsrgqpag spnvraavas hassrrdqlr
       61 taeacirrgi hpdptpttea geyvpggast gtteltkrqr erlealvskk apyslapcrh
      121 prefpaladf pcfrklhedy yrrvrrtfaa erpttsqsat repsahatlg saksrasaks
      181 tgrl
//
LOCUS       XP_077490107             492 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101004 [Amblyomma americanum].
ACCESSION   XP_077490107
VERSION     XP_077490107.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077633981.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..492
                     /product="uncharacterized protein LOC144101004"
                     /calculated_mol_wt=55906
     Region          34..177
                     /region_name="Transposase_21"
                     /note="Transposase family tnp2; cl29371"
                     /db_xref="CDD:475188"
     CDS             1..492
                     /gene="LOC144101004"
                     /coded_by="XM_077633981.1:50..1528"
                     /db_xref="GeneID:144101004"
ORIGIN      
        1 mqterkanle hgdsfvhfgl aegiqhavgq gpapenvelh anidglplhr ssrtalwpil
       61 crvinikkse pfmvsaycga akppclqeyl rpfidevrnl sckglmvkgv hvnvclkavi
      121 cdaparsfvk aitghtgyra cercsqkghy lenrmtfpdl qapqrtdssf rsqedrrhht
      181 gvspftslnv dmvkcfptey mhlvclgvmr rllrnwickg haarlsradr salndklrda
      241 atafpkyfqr kprgteeldr wkatefrtfl lylgpvllks ilsdelykhf lylhvsvrvl
      301 aspqhhrdln qfthdllryf vqefgrlygq kqlvynvhtl ahlaeqcleh gtvdefsafp
      361 fesylgkvkk rlrttnkpla qlsrrmsevr actplstgqv ggevkvgdcf lvdncavviv
      421 dllggnakag ilpngreffr vpidssrmdv rrysalgqet kiwpvsyikn kvrciklqyk
      481 rghvvfpllh lh
//
LOCUS       XP_077490136             327 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077490136
VERSION     XP_077490136.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634010.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..327
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=37354
     Region          <33..289
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; cl46765"
                     /db_xref="CDD:481105"
     CDS             1..327
                     /gene="LOC144101020"
                     /coded_by="XM_077634010.1:6..989"
                     /db_xref="GeneID:144101020"
ORIGIN      
        1 mfdkfydgmd kqwytlllqv rnfgggletg sfqhlwyist dfqlflvgic iiqifrrkhw
       61 aaiavmsgls laccsysawq mynsdyypfp vilgktykat idtlndvyvl ptyhavcyfs
      121 gcitlllllk ykdshisnrt aavlwcista cclacvfnky dwnrgeppeg nwakmafgfg
      181 dkiawsfsls wstfalstgr ggylnsflgw khfvplsrlt lglyvihvpf lnlyynasre
      241 rlyystfsla tlffaalvws lllsillfat ceaptgrlek qlfscrapar dkkpkvvasy
      301 vknndveeak kngdydakfl hntwsrl
//
LOCUS       XP_077490150             175 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101025 [Amblyomma americanum].
ACCESSION   XP_077490150
VERSION     XP_077490150.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634024.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..175
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..175
                     /product="uncharacterized protein LOC144101025"
                     /calculated_mol_wt=19098
     Region          79..>157
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     CDS             1..175
                     /gene="LOC144101025"
                     /coded_by="XM_077634024.1:88..615"
                     /db_xref="GeneID:144101025"
ORIGIN      
        1 mgpdfkfyts akmrishlvc vsiafcclfi tccqaktaet snggptlldt iktalgnamd
       61 nsspemmkkl lyadlssncs lglfklmkgi rnldpwavrl ldatakyptg llqgtqadlg
      121 afdecletia lddygnewvr gqycslyifp pndtslvdal lpamgmshpr vrylc
//
LOCUS       XP_077490175             183 aa            linear   INV 25-NOV-2025
DEFINITION  anaphase-promoting complex subunit 10-like [Amblyomma americanum].
ACCESSION   XP_077490175
VERSION     XP_077490175.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634049.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="anaphase-promoting complex subunit 10-like"
                     /calculated_mol_wt=20374
     Region          2..181
                     /region_name="ANAPC10"
                     /note="Anaphase-promoting complex, subunit 10 (APC10);
                     pfam03256"
                     /db_xref="CDD:367420"
     Site            order(49,76,83,85,148)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176484"
     CDS             1..183
                     /gene="LOC144101036"
                     /coded_by="XM_077634049.1:185..736"
                     /db_xref="GeneID:144101036"
ORIGIN      
        1 mnassaqdps slerqgnfre vgaeavwsls sclpgygvdk lrdnsldtyw qsdglqphev
       61 niqfhrktai qavyiyvdhk rdetytpkri avkvgstfhd lrvvdtvdln eptgwvhiat
      121 qdsdgrpvra yhvqiavlan hkngqdthlr qiklyspvkr aavsvlpgvn ftsaefiafs
      181 sir
//
LOCUS       XP_077490213             132 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101078 [Amblyomma americanum].
ACCESSION   XP_077490213
VERSION     XP_077490213.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634087.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..132
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..132
                     /product="uncharacterized protein LOC144101078"
                     /calculated_mol_wt=14735
     Region          2..>132
                     /region_name="PDDEXK_nuclease-like"
                     /note="PDDEXK family nucleases; cl40440"
                     /db_xref="CDD:477358"
     CDS             1..132
                     /gene="LOC144101078"
                     /coded_by="XM_077634087.1:114..512"
                     /db_xref="GeneID:144101078"
ORIGIN      
        1 msrkkwtekg lanltamkdl srvrairygi anesdailry kdtmiaaghp veiincgifv
       61 nptkpclgvs pdaiafdpce pfpwgtvevk cpyslkdatk edllsqdffv efdenclptl
      121 kkdhehymqv lg
//
LOCUS       XP_077490226             140 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101087 [Amblyomma americanum].
ACCESSION   XP_077490226
VERSION     XP_077490226.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634100.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..140
                     /product="uncharacterized protein LOC144101087"
                     /calculated_mol_wt=16234
     CDS             1..140
                     /gene="LOC144101087"
                     /coded_by="XM_077634100.1:35..457"
                     /db_xref="GeneID:144101087"
ORIGIN      
        1 mdntialydt indrsmkcly ctrpvyvpeq rfavyvyhyp nkknaswnek napylnltes
       61 dlgyfsfgpc eepekqgraq tlyfdgkscy vgrlplsgty hcllflkkgy edsvpeecvk
      121 qydekcgpkk ytlynkdycs
//
LOCUS       XP_077490252             489 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101104 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077490252
VERSION     XP_077490252.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634126.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..489
                     /product="uncharacterized protein LOC144101104 isoform X1"
                     /calculated_mol_wt=54219
     Region          149..226
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(151,153,155,180..182,219)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133137"
     Site            151..153
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133137"
     Site            151
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133137"
     Site            order(180..183,187..190)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133137"
     CDS             1..489
                     /gene="LOC144101104"
                     /coded_by="XM_077634126.1:5..1474"
                     /db_xref="GeneID:144101104"
ORIGIN      
        1 mtrdqivfgt hdakapekll ansqltlqka elckaaeaas aqqevwlreq kqidpvrrit
       61 agsnewlpar ykcrkcgrah gprkcpafgk icracrkknh faeccrtktq igelqrtqed
      121 feilsvnnmt sqadctvktk vesmlaelkv gtgsranllp faayakmhpr pplkpssavl
      181 rsyganqikd lgvvrkevtl dervftqdff ivrkegqail glqacealgq vsrqvhsvwq
      241 sssekfvnev rhlfsgigcv krryrmvlhd garpvvqpar rvpltgpere atrgagahga
      301 wrhhrqsnrt hslgkrllla dmlsrasssr gshsvcpddl vhaessscsr tcclelpave
      361 eaipsapmtw ctptsplegg qtpaailqgh rlrttlpdyr sgpahkvvkh pqlqsparpl
      421 salndadfvs vrsgtwatkg qvlksstypr syhvmleeer tlrrnrkhll ptgeafqldf
      481 shgndvgel
//
LOCUS       XP_077490262             373 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101104 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077490262
VERSION     XP_077490262.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634136.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..373
                     /product="uncharacterized protein LOC144101104 isoform X2"
                     /calculated_mol_wt=41356
     Region          149..226
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(151,153,155,180..182,219)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133137"
     Site            151..153
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133137"
     Site            151
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133137"
     Site            order(180..183,187..190)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133137"
     CDS             1..373
                     /gene="LOC144101104"
                     /coded_by="XM_077634136.1:5..1126"
                     /db_xref="GeneID:144101104"
ORIGIN      
        1 mtrdqivfgt hdakapekll ansqltlqka elckaaeaas aqqevwlreq kqidpvrrit
       61 agsnewlpar ykcrkcgrah gprkcpafgk icracrkknh faeccrtktq igelqrtqed
      121 feilsvnnmt sqadctvktk vesmlaelkv gtgsranllp faayakmhpr pplkpssavl
      181 rsyganqikd lgvvrkevtl dervftqdff ivrkegqail glqacealgq vsrqvhsvwq
      241 sssekfvnev rhlfsgigcv krryrmvlhd garpvvqpar rvpltgpere atrgagahga
      301 wrhhrqsnrt hslgkrllla dmlsrasssr gshsvcpddl vhaessscsr tcclelpave
      361 eaipsapmtw ctp
//
LOCUS       XP_077490270             224 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101104 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077490270
VERSION     XP_077490270.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634144.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..224
                     /product="uncharacterized protein LOC144101104 isoform X3"
                     /calculated_mol_wt=24404
     CDS             1..224
                     /gene="LOC144101104"
                     /coded_by="XM_077634144.1:677..1351"
                     /db_xref="GeneID:144101104"
ORIGIN      
        1 mvlhdgarpv vqparrvplt gpereatrga gahgawrhhr qsnrthslgk rllladmlsr
       61 asssrgshsv cpddlvhaes sscsrtccle lpaveeaips apmtwctpts pleggqtpaa
      121 ilqghrlrtt lpdyrsgpah kvvkhpqlqs parplsalnd adfvsvrsgt watkgqvlks
      181 styprsyhvm leeertlrrn rkhllptgea fqldfshgnd vgel
//
LOCUS       XP_077490303             319 aa            linear   INV 25-NOV-2025
DEFINITION  3-alpha-hydroxysteroid sulfotransferase-like [Amblyomma
            americanum].
ACCESSION   XP_077490303
VERSION     XP_077490303.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634177.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..319
                     /product="3-alpha-hydroxysteroid sulfotransferase-like"
                     /calculated_mol_wt=37221
     Region          35..293
                     /region_name="Sulfotransfer_1"
                     /note="Sulfotransferase domain; pfam00685"
                     /db_xref="CDD:425820"
     CDS             1..319
                     /gene="LOC144101137"
                     /coded_by="XM_077634177.1:165..1124"
                     /db_xref="GeneID:144101137"
ORIGIN      
        1 maprrpfrrv vdgvprcawi dpdtyresqk fqaekgdliq stypksgtyw mqyitqlilk
       61 ggepvrtyde ftsnthaley mkhdgwkpkl pmrmffthlp lhletmraha kyiyaarnpw
      121 dvcvsfyhmm tdvsiwnfqd atfgefldaf letdlgygsy fdhvasgyal kdepnvffvt
      181 yeemkrntrg tilrlarflg ecyssalean etllqniiew sspeytrdil iidfaenknp
      241 awknifhrnk lsckrghegd knkfpmvkta kiggwkehft peqlaclekk iqeegnkasf
      301 idlwkdiree anvmsqgll
//
LOCUS       XP_077490314             317 aa            linear   INV 25-NOV-2025
DEFINITION  sulfotransferase 1B1-like [Amblyomma americanum].
ACCESSION   XP_077490314
VERSION     XP_077490314.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634188.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..317
                     /product="sulfotransferase 1B1-like"
                     /calculated_mol_wt=36843
     Region          <93..294
                     /region_name="Sulfotransfer_1"
                     /note="Sulfotransferase domain; cl21551"
                     /db_xref="CDD:473908"
     CDS             1..317
                     /gene="LOC144101148"
                     /coded_by="XM_077634188.1:132..1085"
                     /db_xref="GeneID:144101148"
ORIGIN      
        1 magrrpyrqi idgeprcpwl npaifqksls fratkgdlvq ssfvksgahy vqyiiqlilk
       61 hgepvrtyye ftsntrlley mdcadwtssl pmrlffthqp lrretmndea kyvyvarnpw
      121 dvcaslfrmi tdisiyrfqd gtfeeflepf megdlgygdy fdhvasgyaq kdksnvfflt
      181 yeelkkdtrg tvirvayflg dcygqaleed eqvlenilew skpenmrkvi vwdlkgnqta
      241 gwddlfgrnd lsskhghegd hakyslvkea rvgswkdvft pnqlvrfeka iknkegkncf
      301 mhlwndicqe aieissa
//
LOCUS       XP_077490324             319 aa            linear   INV 25-NOV-2025
DEFINITION  sulfotransferase 2A1-like [Amblyomma americanum].
ACCESSION   XP_077490324
VERSION     XP_077490324.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634198.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..319
                     /product="sulfotransferase 2A1-like"
                     /calculated_mol_wt=37513
     Region          35..294
                     /region_name="Sulfotransfer_1"
                     /note="Sulfotransferase domain; pfam00685"
                     /db_xref="CDD:425820"
     CDS             1..319
                     /gene="LOC144101156"
                     /coded_by="XM_077634198.1:1..960"
                     /db_xref="GeneID:144101156"
ORIGIN      
        1 msgrrpyrqi idgvprsafl nqdvlrksls fraakgdivq ctypksgsnw lqyltqliit
       61 kgkpilnyne ytrnhrliey tecedwksdl pqrllfthlp lrleamnqda kyiymarnpw
      121 dvcvsqyrmm sdlsifrfqd gsfeefvqpf iegdlghedy fdhvasgyal rdepnvfflt
      181 yeelkqdtrr tvlrlvhflg eeygraledd eqilanvlew sqpenmrkvv vmdlkenesp
      241 ewkdlftkkk mtskngyegd knkyplvkea kvgcwknyft pelltrfeak iqakgekasf
      301 melwadihde aiamsrmra
//
LOCUS       XP_077490336             319 aa            linear   INV 25-NOV-2025
DEFINITION  sulfotransferase 2A8-like [Amblyomma americanum].
ACCESSION   XP_077490336
VERSION     XP_077490336.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634210.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..319
                     /product="sulfotransferase 2A8-like"
                     /calculated_mol_wt=37186
     Region          35..294
                     /region_name="Sulfotransfer_1"
                     /note="Sulfotransferase domain; cl21551"
                     /db_xref="CDD:473908"
     CDS             1..319
                     /gene="LOC144101162"
                     /coded_by="XM_077634210.1:1..960"
                     /db_xref="GeneID:144101162"
ORIGIN      
        1 mpgrrpyrqv idsvprsvfl nqdilrkslt fraakgdivq ctypksgshw lqyltqliit
       61 kgkpiknyse ynrnhravey teccdwksdl parllfthlp fkkealnqda kyiymarnpw
      121 dvcvslyrvm sdlsvyqfqs gtfeeffepf aeddlgfgdy fehvmsgyal rdepnvfflt
      181 yeelkqdtrr tvlrlahflg eeyglalged gqmlanvlew skpenmkkvi vldlkeddas
      241 ewkglftrkk mtskhgyegd ktkfsvvkea rvgswkkyft pelltrfeak iqakgekaff
      301 melwadihde aiamsrmpa
//
LOCUS       XP_077490349             155 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101171 [Amblyomma americanum].
ACCESSION   XP_077490349
VERSION     XP_077490349.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634223.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..155
                     /product="uncharacterized protein LOC144101171"
                     /calculated_mol_wt=17789
     CDS             1..155
                     /gene="LOC144101171"
                     /coded_by="XM_077634223.1:266..733"
                     /db_xref="GeneID:144101171"
ORIGIN      
        1 mykeqtedyk rreaerlmqi vqeslegdrr qdaaeeereh ppprvskddt shaaargfgh
       61 qehglegddv gcergyasmp lsasslgega cttgteaeqs veeenweldi tpgtsgyipr
      121 ghgyfqrarh wsrrtrrrmy keqtedykrr eakld
//
LOCUS       XP_077490372             114 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101186 [Amblyomma americanum].
ACCESSION   XP_077490372
VERSION     XP_077490372.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634246.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..114
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..114
                     /product="uncharacterized protein LOC144101186"
                     /calculated_mol_wt=12167
     CDS             1..114
                     /gene="LOC144101186"
                     /coded_by="XM_077634246.1:100..444"
                     /db_xref="GeneID:144101186"
ORIGIN      
        1 mrtsttssph mtatvkklfg ecvaqqhlqt eqletgmdll gclqqapttp apqpepgrhe
       61 epqchedear rclneaamdl ptsfgfkqgd amleqpactl parggppgrl ptas
//
LOCUS       XP_077490386             199 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101189 [Amblyomma americanum].
ACCESSION   XP_077490386
VERSION     XP_077490386.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634260.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..199
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..199
                     /product="uncharacterized protein LOC144101189"
                     /calculated_mol_wt=21664
     CDS             1..199
                     /gene="LOC144101189"
                     /coded_by="XM_077634260.1:1272..1871"
                     /db_xref="GeneID:144101189"
ORIGIN      
        1 mspatelvga vadhmatrlp npfdsdgahv teavlslppv alleamvndn epsqddfsse
       61 vpaptsmqaa pspwlnsrpl tdealpvqee clsagrptvl vpvvpscqsv seditpaqia
      121 rtraggprva avertlapev aarikaieae drrkeelhql dlqlrrsqla eqrlknkmqr
      181 kllsldfeik krqlealkr
//
LOCUS       XP_077490462             398 aa            linear   INV 25-NOV-2025
DEFINITION  xaa-Arg dipeptidase-like [Amblyomma americanum].
ACCESSION   XP_077490462
VERSION     XP_077490462.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634336.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..398
                     /product="xaa-Arg dipeptidase-like"
                     /calculated_mol_wt=43116
     Region          11..367
                     /region_name="M20_ACY1L2-like"
                     /note="M20 Peptidase aminoacylase 1-like protein 2-like,
                     amidohydrolase subfamily; cd05672"
                     /db_xref="CDD:349921"
     CDS             1..398
                     /gene="LOC144101248"
                     /coded_by="XM_077634336.1:33..1229"
                     /db_xref="GeneID:144101248"
ORIGIN      
        1 mdflplvdra verhgenlwa lsrflwanpe laleefkahd elcgllerhg fvvhrrhlle
       61 tafraefnap ggtegptvai maeydalpsi ghacghnlia estigaalav meamqttyav
      121 rgklvvlgtp aeencggkem llqrgafkdi dvammahpsv edtlrpnvva rtkttvhfrg
      181 ktahaavcpw egvnaldaav asyvnisllr qhlkpssrih gtimesgsyp niipessklv
      241 ynirgetvse lnelvgkaes cfraaaqatg ctmtmesgmq ykdlvhntvl aklyrkhght
      301 lgltftdddm grvaflgaat dagnvshelp tlhpsfaips tagnhtrpya qaanspeaqa
      361 ptrraaklla ltaldlltdp allaqvkref serkarrt
//
LOCUS       XP_077490485             226 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101261 [Amblyomma americanum].
ACCESSION   XP_077490485
VERSION     XP_077490485.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634359.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..226
                     /product="uncharacterized protein LOC144101261"
                     /calculated_mol_wt=24467
     Region          177..210
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..226
                     /gene="LOC144101261"
                     /coded_by="XM_077634359.1:75..755"
                     /db_xref="GeneID:144101261"
ORIGIN      
        1 mqascvpckr gggnrwiacl kplafraavd giwlrvsccc qvrravdwrp passcsflrs
       61 qyrlalfrrf hrrnasdtsq finqhpsglq gmpvspstls tgrrwtlkva spaevevkre
      121 qpdeyaqvrk aaaaeaaava aapaapaqpw alamwpaavl phhlaaggaa aqerssdpee
      181 lfclslaahl rglpprdrsi ammkmlqtlh hlefgenige aalgep
//
LOCUS       XP_077490505            1183 aa            linear   INV 25-NOV-2025
DEFINITION  neurotrypsin-like [Amblyomma americanum].
ACCESSION   XP_077490505
VERSION     XP_077490505.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634379.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1183
                     /product="neurotrypsin-like"
                     /calculated_mol_wt=131403
     Region          122..216
                     /region_name="SR"
                     /note="Scavenger receptor Cys-rich; smart00202"
                     /db_xref="CDD:214555"
     Region          224..359
                     /region_name="CLECT"
                     /note="C-type lectin (CTL) or carbohydrate-recognition
                     domain (CRD); smart00034"
                     /db_xref="CDD:214480"
     Site            order(323,327,338,344,346..349,352..354)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     Region          367..444
                     /region_name="KR"
                     /note="Kringle domain; smart00130"
                     /db_xref="CDD:214527"
     Site            375
                     /site_type="active"
                     /note="putative domain interaction site [active]"
                     /db_xref="CDD:238056"
     Site            order(390,398,426,428,434)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:238056"
     Region          457..491
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(462,470,481..482)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(474,477,481,487..488)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            484..488
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          <517..569
                     /region_name="PAN_AP_HGF"
                     /note="Subfamily of PAN/APPLE-like domains; present in
                     N-terminal (N) domains of plasminogen/hepatocyte growth
                     factor proteins, and various proteins found in Bilateria,
                     such as leech anti-platelet proteins. PAN/APPLE domains
                     fulfill diverse biological functions...; cd01099"
                     /db_xref="CDD:238532"
     Region          579..610
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(581,589,600..601)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(593,596,600,606..607)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            603..607
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          618..726
                     /region_name="SR"
                     /note="Scavenger receptor Cys-rich; smart00202"
                     /db_xref="CDD:214555"
     Region          731..761
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cl00104"
                     /db_xref="CDD:412164"
     Site            order(736,743,754..755)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(747,750,754,760..761)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            757..761
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          771..872
                     /region_name="SR"
                     /note="Scavenger receptor Cys-rich; smart00202"
                     /db_xref="CDD:214555"
     Region          933..1177
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cd00190"
                     /db_xref="CDD:238113"
     Site            933
                     /site_type="active"
                     /note="cleavage site [active]"
                     /db_xref="CDD:238113"
     Site            order(978,1027,1130)
                     /site_type="active"
                     /db_xref="CDD:238113"
     Site            order(1124,1150,1152)
                     /site_type="other"
                     /note="substrate binding sites [chemical binding]"
                     /db_xref="CDD:238113"
     CDS             1..1183
                     /gene="LOC144101281"
                     /coded_by="XM_077634379.1:67..3618"
                     /db_xref="GeneID:144101281"
ORIGIN      
        1 mtaakwitlm aivvrqsncl ispvypgshf tyqrqngltq trhlpppafr epppspphln
       61 pitpgwsswf shwlgprhan ianpldwapp pyvqparsda eaarraelpt rslvlpasnh
      121 tirlhggrlp hegflemkia gkwqslcgda wdqrmtamac eemgytrgge yiqrkypwlt
      181 srhgfdvdcp kevnkmgqcs vqrilcsstr rikvscfrdy asacnpgehp fqgmchlvvg
      241 tpslshaeak lfcqsrgsrl ltthsdvkss fisallsalh kglerwhtdg vrvdgvwiwe
      301 ttklslaadq prplgsaava strecliler ensqaaqlsy arhgwnaahc senlpficer
      361 pafpigcrhs dgaysgianv tesgkpclrw vharigeivs rnypqshlel qghnycrspd
      421 gdstpwcfvn ehdyehcdip dcsqangpsv tvvvkkcsen efqcspdqci rkewicdgdt
      481 dcrngrdeag cdrllelfqk tpmrrmsrye saryfsasvt kcaakcvnaa aftcrsfsym
      541 asgslcilsd rnvartgslv adarfdyyes tahadnctgm frcrngkcie rqllcdiand
      601 cgdrsdeasc vtrmdfqvrl rggphspgrq aegrvevkvh dhwglvcddn wdaeaanvvc
      661 relgfplgai eatkeshhgl paeesaadif mddvlcvgne tslahctfpg wkkhncfine
      721 tagvrcrtkv cgeselecsg acipyyvvcn eqrdcvdgsd edleceskle vrltggphpl
      781 sgrlelrkhg iwgtvcddgf gpaearvact mlgvanasvt vlpsaaygpg rgpiwlddvs
      841 csgrerslee crssswghnd cahsedvaia ctpahtlpra saiesykdic gmpkyqpkld
      901 qasvlfqhas rnrihfhnsn snrpqwrrit grivdgipah ygahpwlvdv rlrdisgqtm
      961 hwcggtilsp dfilsaahcf kfspnvsqlv vrvgqyklya pdpyelefqv ealrlhesfn
     1021 pdtfyndicv lkirttvthr ilfnefvkpv clptkqdtyn ggsyciiagw gktfssqrrq
     1081 prsavlqtaa vplyhpgece qpwvygnrih rgmfcaghtq ggmdachgds ggplicrsit
     1141 gryavfgiis wgeqcglpnr pgvyvkvqdy ldwiarttde lrg
//
LOCUS       XP_077490517             374 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101290 [Amblyomma americanum].
ACCESSION   XP_077490517
VERSION     XP_077490517.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634391.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..374
                     /product="uncharacterized protein LOC144101290"
                     /calculated_mol_wt=39171
     Region          35..81
                     /region_name="DM"
                     /note="DM DNA binding domain; pfam00751"
                     /db_xref="CDD:459924"
     CDS             1..374
                     /gene="LOC144101290"
                     /coded_by="XM_077634391.1:36..1160"
                     /db_xref="GeneID:144101290"
ORIGIN      
        1 mnggvgaaav faapcasaea aaatsalrpp mpsgrqpkca rcrnhgrrrl vrghkkqcpf
       61 rdctcercel iaerqrvmak qvalrraqaa aesmgdrpvt ddedldplfa dptvgppssr
      121 dsqwnpsgvl apdppsrlar eppsafgagg aghalavswg apgcpgvlde dvrkrrhvqd
      181 alaclvnalq vsgadapplv ylyallrean fsvhrafsml learlevqsl aiqealqkda
      241 agpavhlwpq vpchpssapg mlavpglpsl lapafstplp qalpvsigar ssaaishasa
      301 lvgavspaer rppnhgvrsp ccsgggssde dllldverer drsfglsgsp nasqersrlc
      361 ashrskldpk svlk
//
LOCUS       XP_077490530             389 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101297 [Amblyomma americanum].
ACCESSION   XP_077490530
VERSION     XP_077490530.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634404.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..389
                     /product="uncharacterized protein LOC144101297"
                     /calculated_mol_wt=42177
     Region          28..115
                     /region_name="MADF_DNA_bdg"
                     /note="Alcohol dehydrogenase transcription factor
                     Myb/SANT-like; pfam10545"
                     /db_xref="CDD:463144"
     CDS             1..389
                     /gene="LOC144101297"
                     /coded_by="XM_077634404.1:123..1292"
                     /db_xref="GeneID:144101297"
ORIGIN      
        1 mesagsstsg ggqrstnksw tnekearlis lygthrllwd srhpayynkd srehaikaia
       61 qgldnefnvv nvkdkikslr dyfvkelkke aasrkspavr pyvsrwehfr swdflrsvic
      121 cdprvqsppt adlpsgpldg phcgvkqelq evypvffiae epdmilpssp ecsldedeeq
      181 psstkttsls ppsspvpqat gssglqprdh allrpqpnte hgsspsspsy papdsvtspn
      241 vpppkrarpf trperpqenh rgatpascsg gqpssvessp vpspvatcaa slpvgsessn
      301 pnsltginig ggdgrgdsag dgggdgggga pvpfdldlfe erlfcrqvlm elrqmdryhr
      361 dlaklrirqi ifetkymeld atfrgqggn
//
LOCUS       XP_077490541             418 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101308 [Amblyomma americanum].
ACCESSION   XP_077490541
VERSION     XP_077490541.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634415.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..418
                     /product="uncharacterized protein LOC144101308"
                     /calculated_mol_wt=44116
     Region          28..115
                     /region_name="MADF_DNA_bdg"
                     /note="Alcohol dehydrogenase transcription factor
                     Myb/SANT-like; pfam10545"
                     /db_xref="CDD:463144"
     CDS             1..418
                     /gene="LOC144101308"
                     /coded_by="XM_077634415.1:123..1379"
                     /db_xref="GeneID:144101308"
ORIGIN      
        1 mesagsstsg ggqrstnksw tnekearlis lygthrllwd srhpayynka srehaikaia
       61 qgldnefnvv nvkdkikslr dyfvkelkke aasrkspavr pyvsrwehfr swdflrsvic
      121 cdprvqsppt adlpsgpldg phcgvkkelq evypvffiae epdmilpssp ecsldedeer
      181 psstkttsls ppssplpqas gssglqprdh avlrpqpnte qgsspsspsy papdsvtspn
      241 vpppkrarps tspersqenh rratpascsg gqpssvdssp vpspvatcaa slpvgsessn
      301 ptsltginig ggdgrgdggg gdgsggdgag dnggdvgggd gaggdgagdg gggddagggp
      361 vpfdldlfee glfcrqvlme lrqmdryhrd laklrirqii fetkymdlda afrgqggn
//
LOCUS       XP_077490550             360 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101318 [Amblyomma americanum].
ACCESSION   XP_077490550
VERSION     XP_077490550.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634424.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..360
                     /product="uncharacterized protein LOC144101318"
                     /calculated_mol_wt=39840
     Region          109..155
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          190..350
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..360
                     /gene="LOC144101318"
                     /coded_by="XM_077634424.1:943..2025"
                     /db_xref="GeneID:144101318"
ORIGIN      
        1 mavdaaqkdk scqtddiarc sgtfsvflcs argneactqv nhkivcdaev qckpavvsrq
       61 sgrdsrtscf tgydsvqaca aamkdlcgvp pniyalllsi mprsaertcd vpitdrlvpf
      121 lmklklgity sslavlfcvs rntvsrhvks vlktlsiatq kwiyrppdhv irqtmpncfk
      181 vhyqdcsmii yctevrteqp ytvqqqrvly shykggctlk flvgitpcga vcfcskaygg
      241 rcsdsfitld sgfldlvqsg dvvmadkgfp sikagleevr avlvmppflq gldqfsesev
      301 hetyqiaqer ihveriiqri kmfnilntri pteliavmsd vfhvccvlan lqppvisseq
//
LOCUS       XP_077490591             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101351 [Amblyomma americanum].
ACCESSION   XP_077490591
VERSION     XP_077490591.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634465.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144101351"
                     /calculated_mol_wt=15967
     Region          <1..46
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..141
                     /gene="LOC144101351"
                     /coded_by="XM_077634465.1:134..559"
                     /db_xref="GeneID:144101351"
ORIGIN      
        1 mtgfdldgde ysvlwykgli fnnncnsmhy ysdqpkerka piwvelstec levvedslpd
       61 prllleerep flieatsayk ryamkirall ksyrieskse alcgalskls kyvkgerpfg
      121 ygqsarepgr acgapnarvf l
//
LOCUS       XP_077490617             967 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-4-like [Amblyomma americanum].
ACCESSION   XP_077490617
VERSION     XP_077490617.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634491.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..967
                     /product="protein argonaute-4-like"
                     /calculated_mol_wt=108716
     Region          202..271
                     /region_name="ArgoN"
                     /note="N-terminal domain of argonaute; pfam16486"
                     /db_xref="CDD:465134"
     Region          281..332
                     /region_name="ArgoL1"
                     /note="Argonaute linker 1 domain; pfam08699"
                     /db_xref="CDD:462567"
     Region          338..468
                     /region_name="PAZ"
                     /note="PAZ domain; pfam02170"
                     /db_xref="CDD:460472"
     Region          495..924
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(627,631,643..646,649,658,661,665,669)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(696,698,774,914)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..967
                     /gene="LOC144101362"
                     /coded_by="XM_077634491.1:3..2906"
                     /db_xref="GeneID:144101362"
ORIGIN      
        1 mesaprsqrr scgtgrptgr spgapsalsf aqavqqrvvr aappqilsst aagtavitra
       61 egtngngaht vapsegvptt stsptaapvq arngvgntks qndelsiqei ertlpshfpr
      121 rpaqgklgrp iqltanhfsv eipsgnvfhy dveicsesrk etnkvpekrk yrcistkinr
      181 lviellvkky rvdlsgcmpa fdgrknlytr relnfrertf tvdfeedqri qkfivkiqya
      241 atvnldalqa vfdrrvshvp qevlqavdiv lrhgpsiklt pvgrsffrpp apgeyntlgg
      301 gcevwfgyyt svrpaqwkpm lnvdisataf yeplpvtdfm cklfsdcrre msagdfknlr
      361 dfqnvrlnke lkglrvkvth lpyprkykvv rvtkepakki yfkmedgtrc svadyfqncy
      421 drlkypnlpc iqtgsenhpv ylplevcviv egqhckkkld enqtsemikr taqppakrfn
      481 eirqsvrdlv sssdqclref gikisteptq lkgrvldpps lvfennyigk pregtwelrg
      541 rhfyksatlt rwtllnlsqf aqpdsldnfv kmlirvgqel gmsieqpldv tttdtnrkpv
      601 rnilleeqrk aanlemviiv lakntsyadi kqvaeteigl rtqcvmdnnv ikkcnaalit
      661 nlcqkinakl dginnsllpk ekpkifqkpv iiigadvthp apgdklrpsi aacvgsldsi
      721 pykfhasvrv qmadsaatsr leiikdlkdm mkdtlkafyh atrhkperii fyrdgvsegq
      781 fmevrnrevs airlacqels pnetyepalt fivvqkrhht rfmpandrdg vgkcrnvppg
      841 ttvdsvvthp ldfdfylcsh fgiqgtsrps hyyilwddss ftaddvqyls yylchtyarc
      901 arsvsipapv yyahlaayra knhlmskvdv sssssdssgg kadavstrqy veavkvlesl
      961 ktgmyfv
//
LOCUS       XP_077490637             183 aa            linear   INV 25-NOV-2025
DEFINITION  anaphase-promoting complex subunit 10-like [Amblyomma americanum].
ACCESSION   XP_077490637
VERSION     XP_077490637.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634511.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="anaphase-promoting complex subunit 10-like"
                     /calculated_mol_wt=20429
     Region          8..181
                     /region_name="APC10-like"
                     /note="APC10-like DOC1 domains in E3 ubiquitin ligases
                     that mediate substrate ubiquitination; cl02148"
                     /db_xref="CDD:382862"
     Site            order(49,76,83,85,148)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176484"
     CDS             1..183
                     /gene="LOC144101375"
                     /coded_by="XM_077634511.1:395..946"
                     /db_xref="GeneID:144101375"
ORIGIN      
        1 mnacsaqdps ilerqgdire vgaaavwsls scmpgfgvdq lrdncldtyw qsdgdqphev
       61 niqfhrktai qgvyiyvdye rdetytpkri avkvgstfhd lwvvdtvdln eptgwvhiat
      121 qdsdgrpvra fhvqiavlan hkygqdtrlr qiklyspvmr atvsvlpgvn ftsaeciafs
      181 sir
//
LOCUS       XP_077490658             843 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101390 [Amblyomma americanum].
ACCESSION   XP_077490658
VERSION     XP_077490658.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634532.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..843
                     /product="uncharacterized protein LOC144101390"
                     /calculated_mol_wt=92892
     CDS             1..843
                     /gene="LOC144101390"
                     /coded_by="XM_077634532.1:89..2620"
                     /db_xref="GeneID:144101390"
ORIGIN      
        1 msrelsphap spsdlapttp errerpseew lrcdsampeg ghvvrlhaed nqlaalvsgl
       61 sspwyscrqp ydgqvrkepq efitspsgae avrdrsllas lstdppraip sdviwsefet
      121 stepspalsq lrpqpaktwg tseersvfsa vtektststi yacrlasvpe esrvedassd
      181 glltkisyss estadqaqag slsysmkptk sqvpallars aqssvwpraa cgstwqqnqa
      241 ecevqgsgly gdssaisnli rklkgatttc srrnkksrpr dysarpdvpg gpikrskrvs
      301 akraagrkfa vltpneanmv lnmisalrnt dvadsflpls htisppvkdt anmtmplspe
      361 kqlrpvslke vdpllilsqv qefkksalet arrgsygytp iprysatpss ihsaseitye
      421 lqnlweafqq tynseeapqh lpmvyggans fqqnpdvspq wlqsqrsfpl cssnvteslm
      481 dipppnfpqs eciasleerf ldvpsdssch vdqtmkyeaq sssdeqrsmd kmespsqnnl
      541 sfefidpdvy hsnpiyayas nraqpssgkd phdiqwnigr ldllplttdn ittcealkpg
      601 pvaqerntea sfggdflecs gsirdkqrqn elfsrekgis fddecreils eewvrrresl
      661 lirrsllede lqlkdletkq mnrithaghe igpmwcssep selqveeeav anvipvtqwl
      721 deerppddfe petevqvsag whpvidfsfq aqqnedfpgt saanachsrh ashkcspsgs
      781 sldegqpkll tsrssvldsn lcfldppsps ylalpsiaar sapksddedt dagasavekp
      841 aqn
//
LOCUS       XP_077490669             843 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101396 [Amblyomma americanum].
ACCESSION   XP_077490669
VERSION     XP_077490669.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634543.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..843
                     /product="uncharacterized protein LOC144101396"
                     /calculated_mol_wt=92803
     CDS             1..843
                     /gene="LOC144101396"
                     /coded_by="XM_077634543.1:96..2627"
                     /db_xref="GeneID:144101396"
ORIGIN      
        1 msrelsphap spsdlapttp errerpseew lrcdsampeg ghvvrlhaed nqlaalvsgl
       61 sspwyscrqp ydgqvrkepq efitspsgae avrdrsllas lstdtpkeip sdviwsefet
      121 stepspalsq lrpqpaktwg tseersvfsa vtektststi yacrlasvpe esrvedassd
      181 glltkisyss estadqaqag slsysmkptk sqvpallars pqssvwpraa cgstwqqnqa
      241 ecevqgsgly gdssaisnli rklkgatttc srrnkksrpr dysarpdvpg gpikrskrvs
      301 akraagrkfa vltpneanmv lnmisalrnt dvadsflpls htisppvkdt anmtmplspe
      361 kqlrpvslke vdpllilsqv qefkksalet arrgsygytp iprcsatpss ihsaseitye
      421 lqnlweafqq tynseeapqh lptvyggans fqqnpdvspq wlqsqrsfpl cssnvteslm
      481 dipppnfpqs eciasleerf ldvpndstch vdqtmkyeaq sssdeqrsmd kmespsqnnl
      541 sfefidpdvy hsnpiyacas nraqassgqd phdiqwnigr ldllplttdn vtacealkpg
      601 pvaqerntea sfggdflecs gsvrdkqrqn elfsrekgis fddecreils eewvrrresl
      661 lirrsllede lqlkdletkq mnrithaghe igpmwcssep selqveeeav anvipvtqwl
      721 deerppddfe petevqvsag whpvidfsfq aqqnedfpgt saanachsrh ashkcslsgs
      781 sldegqpkll tsrssvldsn lcfldppsps ylalpsiaar sapksddedt dagvsavekp
      841 aqn
//
LOCUS       XP_077490682             107 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101403 [Amblyomma americanum].
ACCESSION   XP_077490682
VERSION     XP_077490682.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634556.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..107
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..107
                     /product="uncharacterized protein LOC144101403"
                     /calculated_mol_wt=11683
     CDS             1..107
                     /gene="LOC144101403"
                     /coded_by="XM_077634556.1:347..670"
                     /db_xref="GeneID:144101403"
ORIGIN      
        1 mkrqrthsfv apnmennpfe dsvellvrtm aannrqvatd qpqlppsnft dpctsssgpi
       61 nvqphfsgtr nstlpvdlah rvasaplata adggiyemar kkrrchr
//
LOCUS       XP_077490702             107 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101416 [Amblyomma americanum].
ACCESSION   XP_077490702
VERSION     XP_077490702.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634576.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..107
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..107
                     /product="uncharacterized protein LOC144101416"
                     /calculated_mol_wt=11639
     CDS             1..107
                     /gene="LOC144101416"
                     /coded_by="XM_077634576.1:63..386"
                     /db_xref="GeneID:144101416"
ORIGIN      
        1 mkrqrthsfv apnmennpfe dsvellvhtm aannrqvatd epqlppsnft dpctsssgpi
       61 nvqphfsgtr nstlpvdlah rgasaplata sdggiyemar kkrrchr
//
LOCUS       XP_077490731             189 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101433 [Amblyomma americanum].
ACCESSION   XP_077490731
VERSION     XP_077490731.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634605.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..189
                     /product="uncharacterized protein LOC144101433"
                     /calculated_mol_wt=20078
     CDS             1..189
                     /gene="LOC144101433"
                     /coded_by="XM_077634605.1:356..925"
                     /db_xref="GeneID:144101433"
ORIGIN      
        1 mkpslvflss hqadicegmt vvgchcglls sadkaimfvi rrlyqasygd edshqgilge
       61 flsradtrpp etdasdvteg qqspteltnh hasfsgpsme hqqqligdhq rgtprcclgs
      121 sagaafptga vtsaeaalpl rclpavrelr iglrrrllgs tlqatwglgl argvawdgsl
      181 rgkigrlll
//
LOCUS       XP_077490761             636 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101452 [Amblyomma americanum].
ACCESSION   XP_077490761
VERSION     XP_077490761.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634635.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..636
                     /product="uncharacterized protein LOC144101452"
                     /calculated_mol_wt=70883
     CDS             1..636
                     /gene="LOC144101452"
                     /coded_by="XM_077634635.1:78..1988"
                     /db_xref="GeneID:144101452"
ORIGIN      
        1 madqaqadsw sysmkttkse vpaslggsaq ssvwpraacg stwqqnqaec evqgsgfygd
       61 ssaisklirk lrgatttcsr rneksrrrdy salpdlpggp ikrslrvsak raarpkfavl
      121 tpneanmvln mistlrntdv adsvlphyrt ishpfkdtan mtmplspenq lqhvslkevd
      181 pllilsqvqe fkksaletar rdsygytpip rytttpssih saseirdelq slweayqqty
      241 nsaeatqnlp mvyeeanslq qnpdvspqwl qssqrsfplc sshvtenlmd ilppnfpqse
      301 ciasreesfl dvpsdstcyv dhtmkyetqs pseeqrsmdk mespsqinws fgytypgiyh
      361 snpiyayasn caqessrqdp hdiqwnigrl dvlplttdnv tscealrpgp vaqernteds
      421 fggnflecsg svrdkqrqne llsrekgipf ddecteilse ewvrrresll igrslleder
      481 qlkdfetkqm nrithaghes kamrfsseps elqveeeava nairvtqwld kerapddfep
      541 eteaqhgedf pgtsaanpch srhashkrsp cgssldegqp klltsgrsvl dgnlcfldpp
      601 spsylglhsi aarstpesdd edsdagasas ekpsqe
//
LOCUS       XP_077490772             152 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101461 [Amblyomma americanum].
ACCESSION   XP_077490772
VERSION     XP_077490772.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634646.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..152
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..152
                     /product="uncharacterized protein LOC144101461"
                     /calculated_mol_wt=16885
     CDS             1..152
                     /gene="LOC144101461"
                     /coded_by="XM_077634646.1:138..596"
                     /db_xref="GeneID:144101461"
ORIGIN      
        1 maghelhtfg rpdlsasqql csgrasartl wettsyreed cpntpagvrp parsvaafhq
       61 arlresthdv cagvrhqktg laslnieted dqrvvrqqqe nirwlqgrap eanpqdfhar
      121 rhapstsmla dlparflghc trrpglfllp ec
//
LOCUS       XP_077490790             183 aa            linear   INV 25-NOV-2025
DEFINITION  anaphase-promoting complex subunit 10-like [Amblyomma americanum].
ACCESSION   XP_077490790
VERSION     XP_077490790.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634664.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="anaphase-promoting complex subunit 10-like"
                     /calculated_mol_wt=20391
     Region          8..181
                     /region_name="APC10-like"
                     /note="APC10-like DOC1 domains in E3 ubiquitin ligases
                     that mediate substrate ubiquitination; cl02148"
                     /db_xref="CDD:382862"
     Site            order(49,76,83,85,148)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176484"
     CDS             1..183
                     /gene="LOC144101481"
                     /coded_by="XM_077634664.1:407..958"
                     /db_xref="GeneID:144101481"
ORIGIN      
        1 mnacsaqdps ilerqgdire vgaaavwsls scmpgfgvdq lrdncldtyw qsdgdqphev
       61 niqfhrktai qgvyiyvdye rdetytpkri avkvgstfhd lrvvdtvdln eptgwvhiat
      121 qdsdgrpvrt flvqiavlan hnygqdtrlr qiklyspvmr atvsvlpgvn ftsaeciafs
      181 sir
//
LOCUS       XP_077490800             118 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101490 [Amblyomma americanum].
ACCESSION   XP_077490800
VERSION     XP_077490800.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634674.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..118
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..118
                     /product="uncharacterized protein LOC144101490"
                     /calculated_mol_wt=12948
     CDS             1..118
                     /gene="LOC144101490"
                     /coded_by="XM_077634674.1:36..392"
                     /db_xref="GeneID:144101490"
ORIGIN      
        1 mprgpssdny lvflvvrlal nsrfctgfhe rrdsrdqpag fspfpgcsac hassrrhqlr
       61 saravsgvey spilrqplrp antfqdtnel agrsptlvrn tcispgrlvi qgaqvlfh
//
LOCUS       XP_077490803             118 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101490 [Amblyomma americanum].
ACCESSION   XP_077490803
VERSION     XP_077490803.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634677.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..118
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..118
                     /product="uncharacterized protein LOC144101490"
                     /calculated_mol_wt=12948
     CDS             1..118
                     /gene="LOC144101490"
                     /coded_by="XM_077634677.1:36..392"
                     /db_xref="GeneID:144101490"
ORIGIN      
        1 mprgpssdny lvflvvrlal nsrfctgfhe rrdsrdqpag fspfpgcsac hassrrhqlr
       61 saravsgvey spilrqplrp antfqdtnel agrsptlvrn tcispgrlvi qgaqvlfh
//
LOCUS       XP_077490825             174 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101527 [Amblyomma americanum].
ACCESSION   XP_077490825
VERSION     XP_077490825.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634699.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..174
                     /product="uncharacterized protein LOC144101527"
                     /calculated_mol_wt=19535
     CDS             1..174
                     /gene="LOC144101527"
                     /coded_by="XM_077634699.1:17..541"
                     /db_xref="GeneID:144101527"
ORIGIN      
        1 mhlkgaldnr gaidadiaqe deecllsatr lhchpgkkil ttdlsqlrsr gihgcptwni
       61 chdyrpwlyh fpdrrqqkal lvpgsavqtq pqhravfgci sqchtgamvs fvvwqghlqt
      121 alqhqevrle rqsrslvrle largtalrst sghaaelrgs aerpapawrr hhvr
//
LOCUS       XP_077490830             238 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101535 [Amblyomma americanum].
ACCESSION   XP_077490830
VERSION     XP_077490830.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634704.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..238
                     /product="uncharacterized protein LOC144101535"
                     /calculated_mol_wt=26470
     CDS             1..238
                     /gene="LOC144101535"
                     /coded_by="XM_077634704.1:238..954"
                     /db_xref="GeneID:144101535"
ORIGIN      
        1 matehevrlf ahnlwqdrla glqpqprrvd qdnpgsgvav vgsylieehl ivfafaagsv
       61 lgmhlkgald nrgaidaaia qedeecllsa thlhchpgkk ilttdlsqlr srgihgcptw
      121 nicrdyrpwl yhfpdrrqqk allvpgfars vqtqpqdrav fgcisqchtg amvsfvvwqe
      181 hlqtalqhqe vrlarqsssl vrlelardta lrstsghaag lrgsaerpap awrrhhvr
//
LOCUS       XP_077490843             238 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101558 [Amblyomma americanum].
ACCESSION   XP_077490843
VERSION     XP_077490843.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634717.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..238
                     /product="uncharacterized protein LOC144101558"
                     /calculated_mol_wt=26429
     CDS             1..238
                     /gene="LOC144101558"
                     /coded_by="XM_077634717.1:249..965"
                     /db_xref="GeneID:144101558"
ORIGIN      
        1 matehdvrlf ahnlwqdrla glqpqprrvd qdnpgcgvav vgsylieehl ivfafaagsv
       61 lgmhlkgald nrgaidaaia qedeecllsa trlhchpgkk ilttdlsqlr srgihgcptw
      121 nicrdyrpwl yhfldrrqqk allvagfars vqtqpqdrad fgcisqchtg amvsfvvwqg
      181 hlqtalqhqe vrlarqsrsl vrlelargia lrstsghaag lrgsaehpap awrrhhvr
//
LOCUS       XP_077490851             236 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101573 [Amblyomma americanum].
ACCESSION   XP_077490851
VERSION     XP_077490851.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634725.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..236
                     /product="uncharacterized protein LOC144101573"
                     /calculated_mol_wt=26486
     CDS             1..236
                     /gene="LOC144101573"
                     /coded_by="XM_077634725.1:223..933"
                     /db_xref="GeneID:144101573"
ORIGIN      
        1 matehevrlf ahnlwqdrla glqpqprrvd qdnpgsgvav vgsylieehl ivfafaagsv
       61 lgmhlkgald nrgaidaaia qedeecllsa trlhchpekk ilttdlsqlr srgihgcptw
      121 nicrdyrpwl yhfpdrrqkk allvpgfavq tqpqdravfg risqcyngam vsfavwqghl
      181 qtalqhqevr lerqsrslvr lelargtafr starhaaglr gsaersapaw rrhhvr
//
LOCUS       XP_077490866             176 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101594 [Amblyomma americanum].
ACCESSION   XP_077490866
VERSION     XP_077490866.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634740.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..176
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..176
                     /product="uncharacterized protein LOC144101594"
                     /calculated_mol_wt=19659
     CDS             1..176
                     /gene="LOC144101594"
                     /coded_by="XM_077634740.1:333..863"
                     /db_xref="GeneID:144101594"
ORIGIN      
        1 mhlkgaldnr raidaaiaqd deeerllsat rlhchtgkki lttdlsqlrs rgihvcptwn
       61 icrdyrpwly hfpdrrqqka llvpgsavqt qpqdhalfgc isqcdtgvmv sfivwqghlq
      121 tvlqhrevrl epqsrslgrl elarcgsvrp taghaagvrc sgaqrpaqaw pghhvr
//
LOCUS       XP_077490874             204 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101602 [Amblyomma americanum].
ACCESSION   XP_077490874
VERSION     XP_077490874.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634748.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..204
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..204
                     /product="uncharacterized protein LOC144101602"
                     /calculated_mol_wt=22132
     CDS             1..204
                     /gene="LOC144101602"
                     /coded_by="XM_077634748.1:174..788"
                     /db_xref="GeneID:144101602"
ORIGIN      
        1 mvmvlgiqil schprreeel lpgglepqpp dmprrdpggi mvavgrfvdq qqlhgvlasa
       61 vrggaqrlal rgdhtvdsqr edhgahgcgl cgkkiaergv vpppqrlsaa dtdppgpgaq
      121 hprsnhtwwr papqrrglyp dwpiflkqss sfrpwehavc gwigqwegll rqpgcisrlp
      181 walgeaaegt argsgpgqrv eqct
//
LOCUS       XP_077490884             150 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101612 [Amblyomma americanum].
ACCESSION   XP_077490884
VERSION     XP_077490884.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634758.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..150
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..150
                     /product="uncharacterized protein LOC144101612"
                     /calculated_mol_wt=16715
     CDS             1..150
                     /gene="LOC144101612"
                     /coded_by="XM_077634758.1:153..605"
                     /db_xref="GeneID:144101612"
ORIGIN      
        1 maerhpplfn hhstaivpal slpapkavdt eipkftplpd rppttlnstc hrhqlyqike
       61 lwhcdrnaht papllllrnd peqktcgcir asgrqprsnn fkraasarrt kaaalrmlsr
      121 asvsstncgw cclvlcgrgh swrhpecnws
//
LOCUS       XP_077490893             601 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101622 [Amblyomma americanum].
ACCESSION   XP_077490893
VERSION     XP_077490893.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634767.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..601
                     /product="uncharacterized protein LOC144101622"
                     /calculated_mol_wt=66276
     CDS             1..601
                     /gene="LOC144101622"
                     /coded_by="XM_077634767.1:145..1950"
                     /db_xref="GeneID:144101622"
ORIGIN      
        1 mihaggssya qrsvslssaa tstqhqsnhq krccnfgkrq gptydsqlfl mpcrvfeets
       61 srralfiqrl ssnvdgnrsk niqgvrrsll pseywallls lkpqpclvpr yhscsakavv
      121 wpfvaeqqqp vivaererki rdsplyaaaa gsskveghrv hgrrrssypr vekvqsgqlp
      181 hkipstadps trrpgeknlr shrnltrppe qpgcrpvlhl eskarnlppa rphlgevhad
      241 laapaalrll agvvvvrllc tvwtvefwth isccflawvt ahsqaallvl lraagatagt
      301 tlcgtgpkqe tppsrrtwsg dkesvptglv phssvvshal athrrgdeev astswaprss
      361 vgplaltthr sgdegvasai laprssvgpr aqatlrsgdv vvaptscllc ffivprplat
      421 qrsgdeeaap tkwmprsfvv prplathrsg dveaaptrwv phsfvvprpl vshrsgdeet
      481 aptkwvprsi vvpralathr sgdeeagptr wvpysfvvpl plathrsgde eaaptrwvpg
      541 clffrvswlp rgaetrrlpr qagrpahlsf rdrwlpreae trswswqsrc laflwshvyw
      601 l
//
LOCUS       XP_077490907             237 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101640 [Amblyomma americanum].
ACCESSION   XP_077490907
VERSION     XP_077490907.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634781.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..237
                     /product="uncharacterized protein LOC144101640"
                     /calculated_mol_wt=27321
     Region          1..43
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          72..230
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..237
                     /gene="LOC144101640"
                     /coded_by="XM_077634781.1:1..714"
                     /db_xref="GeneID:144101640"
ORIGIN      
        1 mvlvrlrtgm qghelarnfl iseslvnrtf stwiinflqr elrhlttfpt redigphlpn
       61 sfhdfpdtrl vldategriq rpsamyakrq tfspykhynt yqvligctld gcishvsrlw
      121 wgsssdkail easglldkld agdaimmdkg ftfpyfppgi kvyrppfres hekqmpakav
      181 detrrissar vhvkraisrc kefhiidrpf pismidiaeq vfevccllrn frlplir
//
LOCUS       XP_077490929             141 aa            linear   INV 25-NOV-2025
DEFINITION  anaphase-promoting complex subunit 10-like [Amblyomma americanum].
ACCESSION   XP_077490929
VERSION     XP_077490929.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634803.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="anaphase-promoting complex subunit 10-like"
                     /calculated_mol_wt=15956
     Region          9..135
                     /region_name="APC10"
                     /note="APC10 subunit of the anaphase-promoting complex
                     (APC) that mediates substrate ubiquitination; cd08366"
                     /db_xref="CDD:176484"
     Site            order(34,61,68,70,133)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176484"
     CDS             1..141
                     /gene="LOC144101664"
                     /coded_by="XM_077634803.1:160..585"
                     /db_xref="GeneID:144101664"
ORIGIN      
        1 madrnlrwqa awevssfmpg rgvdrlhddc vgtywqsegq lphlvniqll rettvrgvmi
       61 ytiyiedfsy tprrvsvrvg ptlqdlhevh eaqlcapmgw vylptedsag qpvrtsvvql
      121 avlsnhhcge nsrlrlamlh c
//
LOCUS       XP_077491039             136 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101760 [Amblyomma americanum].
ACCESSION   XP_077491039
VERSION     XP_077491039.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634913.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..136
                     /product="uncharacterized protein LOC144101760"
                     /calculated_mol_wt=14192
     CDS             1..136
                     /gene="LOC144101760"
                     /coded_by="XM_077634913.1:144..554"
                     /db_xref="GeneID:144101760"
ORIGIN      
        1 mvrklfalaa lvavatvafa eekpaandkp atqekkeeve srigfgtgfg hvagangygf
       61 nrgaagfnqg sggfnsanay nnvhgfrnre gyrtnhgfdq ttfnrygsgh agfntgfnhg
      121 aggfhnqhgh lggfig
//
LOCUS       XP_077491071             388 aa            linear   INV 25-NOV-2025
DEFINITION  RYamide receptor-like [Amblyomma americanum].
ACCESSION   XP_077491071
VERSION     XP_077491071.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077634945.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..388
                     /product="RYamide receptor-like"
                     /calculated_mol_wt=43525
     Region          12..300
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          12..36
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320513"
     Region          45..67
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320513"
     Region          83..105
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320513"
     Region          126..142
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320513"
     Region          172..194
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320513"
     Region          225..247
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320513"
     Region          268..293
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320513"
     CDS             1..388
                     /gene="LOC144101761"
                     /coded_by="XM_077634945.1:146..1312"
                     /db_xref="GeneID:144101761"
ORIGIN      
        1 mnalgfelqa flialyslta llalggnvmv ivvlvlgrrs srelrlflvn lalsdiamav
       61 fsipftytdf mlgrwifepl fcpvvlfmqh vsvivsvytl taigvdryya imyplsfrwt
      121 rrrgphiila iwlvagmlss lqlvngrake ftwagdtyyd ctedwevnag kvytaviftv
      181 tfmtpmlalt ftyssigwrm wrhtspgnad aqrdqqqlsa kmkvvkmlat vvlmfavcwl
      241 pihlmnlily fdreamlaet aeqqylyiaa ffschwfsma nsfvnpiiyc fmsdnfrvdl
      301 rqlvvgccpl lamraggrsl gkrssygggg slpsstmmls sttsshhhqh hqrcngfyah
      361 sptsvvalkt lgdcvateaa tvrlpvye
//
LOCUS       XP_077491145             553 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome P450 4V2-like [Amblyomma americanum].
ACCESSION   XP_077491145
VERSION     XP_077491145.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635019.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..553
                     /product="cytochrome P450 4V2-like"
                     /calculated_mol_wt=63146
     Region          98..533
                     /region_name="CYP4"
                     /note="cytochrome P450 family 4; cd20628"
                     /db_xref="CDD:410721"
     Site            order(129,147..148,155,159,166,343,346..347,350,412,416,
                     477..479,483..487,490..491,495)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410721"
     Site            order(148,345..346,350,415,417)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410721"
     CDS             1..553
                     /gene="LOC144101799"
                     /coded_by="XM_077635019.1:306..1967"
                     /db_xref="GeneID:144101799"
ORIGIN      
        1 malqwsshdv aatshldavf mtvapclfgi lvcyvvfiat arwfktwial rplpgpwdgi
       61 pfwfstvayw krsrqfsakd aavgffsvir elcdtytgkt fkayigmtpi vvlhtpeave
      121 tllsnkesla kphvydfiaa wlgpnnllts rgepwrqkrk lftpafharv leiymdvfhk
      181 nsdgfigqie qflrknpnep fscfqglqkc fvdtmarvcm gvelntqqdd tnffgncfnr
      241 lsyltavrgc rpwlwiqrvy dmtkegrvfk dtvekmqmfs ytvlrerkei llknqsvntk
      301 digmkkstsm pessslfldc lltysikdpa ysieevkndi dsiifagtds tasgvswtiy
      361 llglhpvkla kvheeldqll ggdidsaits ddllhlnyle cclkeslrly ppfplfgrkl
      421 ehdmvidghn lpegatcfvn lyslhrdprh fhepnsflpe rflsgefser hpysyvpfsa
      481 gpknclgqrf fvleakllla kvlskfsves tkpveelkia yevvlkargg lrvwfrkrnm
      541 vnskqstrkg ial
//
LOCUS       XP_077491154              82 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101809 [Amblyomma americanum].
ACCESSION   XP_077491154
VERSION     XP_077491154.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635028.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..82
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..82
                     /product="uncharacterized protein LOC144101809"
                     /calculated_mol_wt=7942
     CDS             1..82
                     /gene="LOC144101809"
                     /coded_by="XM_077635028.1:116..364"
                     /db_xref="GeneID:144101809"
ORIGIN      
        1 mkvtailatl allvavasag ylggyglgyg gyglgyglgy ggygyglghv gyglgyggyg
       61 lgyggyglgl gyggygfglk hy
//
LOCUS       XP_077491177             599 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101829 [Amblyomma americanum].
ACCESSION   XP_077491177
VERSION     XP_077491177.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635051.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..599
                     /product="uncharacterized protein LOC144101829"
                     /calculated_mol_wt=67480
     Region          141..284
                     /region_name="Transposase_21"
                     /note="Transposase family tnp2; cl29371"
                     /db_xref="CDD:475188"
     CDS             1..599
                     /gene="LOC144101829"
                     /coded_by="XM_077635051.1:112..1911"
                     /db_xref="GeneID:144101829"
ORIGIN      
        1 mpsvqselne qayvsescae slssehtats cdssdetsst nsltaenvem gqpreespel
       61 cyrhlstaqe lasiaskhnl thacindlld fyrrrgivel pkdartimqt erkanlehgd
      121 sfvhfglaeg iqhavgqgpa penvelhani dglplhrssq talwpilctv inikksepfm
      181 vsaycgagkp pclqeylrpf idevrnlscn glmvkgvhvn vclkavicda parsfvkais
      241 ghtgyhacer csqkghylen rmtfpdlhap qrtdssfrsq edrrhhtgvs pftslnvdmv
      301 kcfpteymhl vclgvmrrll rnwickghaa rlsradrsal ndklreaata fpkyfqrkpr
      361 gteeldrwka tefrtfllyl gpvllksils delykhflyl hvsvrvlasp qhhrdlnqfa
      421 hdllryfvqe fgrlygqkql vynvhtlahl aeqclehgtl defsafpfes ylgkvkkrlr
      481 ttnkplaqls rrmsevract plstgqvggd vkvgdcflvd ncavvivdll ggnakagilp
      541 ngreffrvpi dssrmdvrcy salgqetkiw pvsfiknkvr ciklqykrgh vvfpllhlq
//
LOCUS       XP_077491188              67 aa            linear   INV 25-NOV-2025
DEFINITION  protein lin-52 homolog [Amblyomma americanum].
ACCESSION   XP_077491188
VERSION     XP_077491188.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635062.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..67
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..67
                     /product="protein lin-52 homolog"
                     /calculated_mol_wt=7309
     Region          <1..44
                     /region_name="LIN52"
                     /note="Retinal tissue protein; pfam10044"
                     /db_xref="CDD:462950"
     CDS             1..67
                     /gene="LOC144101838"
                     /coded_by="XM_077635062.1:50..253"
                     /db_xref="GeneID:144101838"
ORIGIN      
        1 mvhvlaslsp ktllgivknl qdmayqlsve elremtrara lnifskdkac drnhetngls
       61 gpessqp
//
LOCUS       XP_077491209             493 aa            linear   INV 25-NOV-2025
DEFINITION  chitinase-3-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077491209
VERSION     XP_077491209.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635083.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..493
                     /product="chitinase-3-like protein 1"
                     /calculated_mol_wt=55010
     Region          <370..485
                     /region_name="GH18_chitinase-like"
                     /note="The GH18 (glycosyl hydrolase, family 18) type II
                     chitinases hydrolyze chitin, an abundant polymer of
                     beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a
                     major component of the cell wall of fungi and the
                     exoskeleton of arthropods. Chitinases have...; cl10447"
                     /db_xref="CDD:471972"
     CDS             1..493
                     /gene="LOC144101855"
                     /coded_by="XM_077635083.1:42..1523"
                     /db_xref="GeneID:144101855"
ORIGIN      
        1 mdamirvpsg tskrvfvafl ccgilvvlml slstlvmvrp dafpwnhvnr sahdswllre
       61 headvsvhaq ditgpedrfa ttdtppvqis mqappqrtrd rgsfkllpae lynnsshqyf
      121 cfyqaraerp esagvlfgls vfpyhlctha vfccshvngs smnieaspea dgfprylrgl
      181 nphirpmmgf ngkmpasmlt gsdwiflesa lvwltprgye gavllwrvsl etlaarrgyt
      241 dllermtrrf ekerlilsvv vdagdtlkqr ldlgelaasl ptsaegptaa sillhpivge
      301 pplartrgve dqrsavlyek nrlqevlkql qstmkqteee plshslgvav rgyhspsvsd
      361 eaatklcyvf sfagvtykfw dsnvtgvqel vygpgdpgps tktpgelayy eicneiwdss
      421 qvfefgvvsk qganwvshlt efvvpplarf lrykfrarcf gvwnlwhddf agvcgggryp
      481 lmrnlfgvfs epr
//
LOCUS       XP_077491237             413 aa            linear   INV 25-NOV-2025
DEFINITION  kinesin-like protein KLP2 [Amblyomma americanum].
ACCESSION   XP_077491237
VERSION     XP_077491237.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635111.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..413
                     /product="kinesin-like protein KLP2"
                     /calculated_mol_wt=46015
     Region          <44..>155
                     /region_name="ClpA"
                     /note="ATP-dependent Clp protease, ATP-binding subunit
                     ClpA [Posttranslational modification, protein turnover,
                     chaperones]; COG0542"
                     /db_xref="CDD:440308"
     Region          111..402
                     /region_name="Motor_domain"
                     /note="Myosin and Kinesin motor domain; cl22853"
                     /db_xref="CDD:473979"
     Site            order(161..168,276..277,312..317)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:276814"
     CDS             1..413
                     /gene="LOC144101885"
                     /coded_by="XM_077635111.1:598..1839"
                     /db_xref="GeneID:144101885"
ORIGIN      
        1 mgsdataesq teerraaesr rdellktlha ttsmraqger llaskagsle alrselmelq
       61 mekeslrrkr lrvadstqal lrdledvrea ikekkeelta ltqerrelle ktlelkgrgr
      121 grftvdgvfa asttqdhfyd qirelvqhvv dghhvsifay gqtgagktht megpaaeeay
      181 aergivprav eqlwdsvqsq lqtshdikit vveiyneell dvlgfsnegl ssadrkvclm
      241 ehestvelrn atevqitska qlsdivehvr mhratastvf narssrshvv yrvdvracge
      301 egagqgrlnl idlagsetee etggddarrr eaaciksslv gletvflela ageyvpfrrs
      361 kltlllknsl egrgkvlfiv nispraedis etmkslhfat svsekrlkkk kpe
//
LOCUS       XP_077491258             293 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101908 [Amblyomma americanum].
ACCESSION   XP_077491258
VERSION     XP_077491258.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635132.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..293
                     /product="uncharacterized protein LOC144101908"
                     /calculated_mol_wt=30370
     CDS             1..293
                     /gene="LOC144101908"
                     /coded_by="XM_077635132.1:21..902"
                     /db_xref="GeneID:144101908"
ORIGIN      
        1 mqqgvaaspq pravsggptp aaapsaqpsg srpsygaaag sakvaasspw pasaaaavsg
       61 sqpaaaalpq sppgavtert aeagdqatpg ddvtikgpcd grnmmhaswt saegnarprk
      121 qirlsdfmpv madsslplss tgpsfrkspr aspceedmra aaasaeaaea desldatlvv
      181 ngseasqsdd svsvesasss stmtpenatn nrspsdgrpa raaepdlpea llrrledmin
      241 erarhmeayl qqvhiqlilm qhmfmgeiqt mlqevldevl vlltiidemr epi
//
LOCUS       XP_077491279             141 aa            linear   INV 25-NOV-2025
DEFINITION  anaphase-promoting complex subunit 10-like [Amblyomma americanum].
ACCESSION   XP_077491279
VERSION     XP_077491279.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635153.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="anaphase-promoting complex subunit 10-like"
                     /calculated_mol_wt=15941
     Region          9..135
                     /region_name="APC10"
                     /note="APC10 subunit of the anaphase-promoting complex
                     (APC) that mediates substrate ubiquitination; cd08366"
                     /db_xref="CDD:176484"
     Site            order(34,61,68,70,133)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176484"
     CDS             1..141
                     /gene="LOC144101922"
                     /coded_by="XM_077635153.1:193..618"
                     /db_xref="GeneID:144101922"
ORIGIN      
        1 madrnlrwqa awevssfmpg rgvdrlhddc vgtywqsegq lphlvniqll rettvrgvmi
       61 ytiyiedfsy tprrvsvrvg ptlqdlhevh eaelsapmgw vylptedsag qpvrtsvvql
      121 avlsnhhcge nsrlrlamlh c
//
LOCUS       XP_077491306             919 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101939 [Amblyomma americanum].
ACCESSION   XP_077491306
VERSION     XP_077491306.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635180.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..919
                     /product="uncharacterized protein LOC144101939"
                     /calculated_mol_wt=101721
     Region          <830..>893
                     /region_name="Jnk-SapK_ap_N"
                     /note="JNK_SAPK-associated protein-1; pfam09744"
                     /db_xref="CDD:462875"
     CDS             1..919
                     /gene="LOC144101939"
                     /coded_by="XM_077635180.1:325..3084"
                     /db_xref="GeneID:144101939"
ORIGIN      
        1 mdnverlgde hsapvafper kdcaviadqp rsldaaaaiy sgdcnedcsp sikqavtspl
       61 ldlfkddgsg rhlainvnnv psetqapcvv ehtfvetctv pangdlkngs wkkrmsgelq
      121 arggvstvee sepkgasqls tlaapvqsmg fhadetpdif kpnpsssgta lrssspfkdd
      181 vfnithsssd llaavnedaa espvtgnavg kvpplssvtp avgekltkgg apacrsrncr
      241 qltvdemacl sdkllvldsl tqppveknfp aeenkgnkak qnpgavcpkf slrglqpeel
      301 rrqrdeaynr vdsleelitv lkvfydsdfk fmlsnlrkle eenrrlsgan eetrllmqel
      361 lsmrgdsslp dpvcdqlely mettpllrsv apasgdnlte agapagssrd chelpmgema
      421 crsdkllpvd srtqapfven leavhnygkk gkkkpspvcr kfsllglqpe elrrqkdqll
      481 lrqrdeaydr vdrleneitf lkefsdrdsk fmlnkvrkle eenrrlsgan eeqkllmlel
      541 lsrrggsslp dagcdqvelq vatkpplrcv tpaagdnlte agapagssrn crqlpmgema
      601 cpsdkllsad srtqppfven ldavhnigkk gkqkpgpvcp klslrglqpe elrrqkdqll
      661 rrqrdeaydr vdrlekevtf mqeyndrele fmlnkvrkle eenhrlsgan aeqrllmqvl
      721 lsrrgdcslp ddvcdlvelq vetkpplrcv tpangdnlse agapagsssn clqqsmgema
      781 cpsdkllpvd srtqppiekn lhavqnngkk gkqepgavcp ksllrglqpe elrrqndhll
      841 rrqrdeaydr adslekeiis lqdisdrdfe fmlnkvrkle eenrrlsgan aeqrllmqel
      901 lsrrgdssls davcdeler
//
LOCUS       XP_077491316             211 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101945 [Amblyomma americanum].
ACCESSION   XP_077491316
VERSION     XP_077491316.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635190.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..211
                     /product="uncharacterized protein LOC144101945"
                     /calculated_mol_wt=23685
     Region          7..91
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     Region          162..196
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..211
                     /gene="LOC144101945"
                     /coded_by="XM_077635190.1:161..796"
                     /db_xref="GeneID:144101945"
ORIGIN      
        1 masfneklvq evkkhtqlwd qhsklhkeag yreaawmeia aalavtveec qtrwrtlrdt
       61 ylkrkkrrri dskgqwsvld relgflddfl rprtrrstln aaapadveve qeqpgedaem
      121 reaavaaaaa ppqpwplamr paavmphrla aggaaaqals sdpeelfcls laarlkrllp
      181 rerhmarmkm lqtlhdlesg enigefalgd p
//
LOCUS       XP_077491321             211 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144101954 [Amblyomma americanum].
ACCESSION   XP_077491321
VERSION     XP_077491321.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635195.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..211
                     /product="uncharacterized protein LOC144101954"
                     /calculated_mol_wt=23581
     Region          7..91
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     Region          162..196
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..211
                     /gene="LOC144101954"
                     /coded_by="XM_077635195.1:105..740"
                     /db_xref="GeneID:144101954"
ORIGIN      
        1 masfneklvq evkkhtqlwd qhsklhkeag yreaawmeia aalavtveqc qtrwrtlrdt
       61 ylkrkkrrra gangqwnvle rdlgflddfl rprtrrstln aaapadveve qeqpgedaem
      121 reaavaaaaa ppqpwplamr paavmphrla aggaaaqals sdpeelfcls laarlkrllp
      181 rerhmarmkm lqtlhdlesg enigefalgd p
//
LOCUS       XP_077491330             212 aa            linear   INV 25-NOV-2025
DEFINITION  aurora kinase A-like [Amblyomma americanum].
ACCESSION   XP_077491330
VERSION     XP_077491330.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635204.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..212
                     /product="aurora kinase A-like"
                     /calculated_mol_wt=23916
     Region          <1..196
                     /region_name="STKc_Aurora"
                     /note="Catalytic domain of the Serine/Threonine kinase,
                     Aurora kinase; cd14007"
                     /db_xref="CDD:270909"
     Site            order(30,32,69,71,73,90,104..107)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270909"
     Site            order(86..95,98..107)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270909"
     CDS             1..212
                     /gene="LOC144101961"
                     /coded_by="XM_077635204.1:106..744"
                     /db_xref="GeneID:144101961"
ORIGIN      
        1 mrhenilely nwfhdesrvy lileyaphgt lfqklvkekh lndvsaatfi dqmckalkyc
       61 hglgvihrdi kpenlllgin eelkvadfgw aaqgastrrk tfcgtldyla peilaggsyd
      121 mkvdhwclgv layellvgcp pfetesvakt ralikkanit fprhvcaaar glirkllqle
      181 adkrmsldev lehpwvsana irpeksktsk kn
//
LOCUS       XP_077491387             587 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102010 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077491387
VERSION     XP_077491387.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635261.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..587
                     /product="uncharacterized protein LOC144102010 isoform X1"
                     /calculated_mol_wt=64139
     CDS             1..587
                     /gene="LOC144102010"
                     /coded_by="XM_077635261.1:61..1824"
                     /db_xref="GeneID:144102010"
ORIGIN      
        1 masasrrsaq ptslsyasah asqqqpcrrp lpgilkttpg ffsfpkgspt tktlrfqips
       61 ptpgslaqly aqeeaeqdlv latgeaatga kteksdssss psaeetsydk fhlrghkrrr
      121 slsprrrscs sasgadsvvp ywmpkeiqhy flnypdngee gsgaagapct kdapsalqci
      181 gseqpprnde fsswrrlagq prcalatlcf vaalalifvg tttnvyvgmv hgargdgrtp
      241 vvagagagga dsagakkepl gskavprsmg dypdalkrrr aetaeptrrh avaafdskee
      301 reettggdae megrsrhdev gtdagddlpr pvtmdymgta rdvaeivhef ssseddahrs
      361 paqtrascea pmfsycpipr iefyynrvtn acvstaldga gvcsrgpnkf psrhtcrqrc
      421 ieygrpaknc rhkaifkect rqdvsqswwy hsgnrchrwt fprglcpasg sdvfrtfgec
      481 wrrcvsdeqh snsrvrfyel tggwllgqrr gprpcrlprt evcraeslky ayfadssprp
      541 aaagsrvprc hsvpvanqlg hrcleganrf rtakachrtc vtnsgrh
//
LOCUS       XP_077491398             466 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102010 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077491398
VERSION     XP_077491398.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635272.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..466
                     /product="uncharacterized protein LOC144102010 isoform X2"
                     /calculated_mol_wt=51115
     CDS             1..466
                     /gene="LOC144102010"
                     /coded_by="XM_077635272.1:61..1461"
                     /db_xref="GeneID:144102010"
ORIGIN      
        1 masasrrsaq ptslsyasah asqqqpcrrp lpgilkttpg ffsfpkgspt tktlrfqips
       61 ptpgslaqly aqeeaeqdlv latgeaatga kteksdssss psaeetsydk fhlrdgrtpv
      121 vagagaggad sagakkeplg skavprsmgd ypdalkrrra etaeptrrha vaafdskeer
      181 eettggdaem egrsrhdevg tdagddlprp vtmdymgtar dvaeivhefs sseddahrsp
      241 aqtrasceap mfsycpipri efyynrvtna cvstaldgag vcsrgpnkfp srhtcrqrci
      301 eygrpakncr hkaifkectr qdvsqswwyh sgnrchrwtf prglcpasgs dvfrtfgecw
      361 rrcvsdeqhs nsrvrfyelt ggwllgqrrg prpcrlprte vcraeslkya yfadssprpa
      421 aagsrvprch svpvanqlgh rcleganrfr takachrtcv tnsgrh
//
LOCUS       XP_077491432             222 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102036 [Amblyomma americanum].
ACCESSION   XP_077491432
VERSION     XP_077491432.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635306.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..222
                     /product="uncharacterized protein LOC144102036"
                     /calculated_mol_wt=24213
     CDS             1..222
                     /gene="LOC144102036"
                     /coded_by="XM_077635306.1:462..1130"
                     /db_xref="GeneID:144102036"
ORIGIN      
        1 mpfdpfense qvagsrekiy kmvrhhnsqs gadrrtcdfe velaeifgka hsinppyllg
       61 pglvqsiqdr qaepaaspqp gpsrvtatas pipgssqlst srgsttagds qppspstlps
      121 prqqqpgpar krprrdttra vlesaithle vaeanrqqrh rermalqerk vaaeerrtaa
      181 lervaaaler qvlvslfspi raptrspihs pihspsppyy na
//
LOCUS       XP_077491441              91 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102045 [Amblyomma americanum].
ACCESSION   XP_077491441
VERSION     XP_077491441.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635315.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..91
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..91
                     /product="uncharacterized protein LOC144102045"
                     /calculated_mol_wt=9617
     Region          2..>49
                     /region_name="PWWP"
                     /note="PWWP (Pro-Trp-Trp-Pro) domain; cl02554"
                     /db_xref="CDD:470613"
     Site            order(14,17,41)
                     /site_type="other"
                     /note="putative methyl-histone binding site [polypeptide
                     binding]"
                     /db_xref="CDD:438958"
     Site            15..18
                     /site_type="other"
                     /note="PWWP sequence motif"
                     /db_xref="CDD:438958"
     CDS             1..91
                     /gene="LOC144102045"
                     /coded_by="XM_077635315.1:272..547"
                     /db_xref="GeneID:144102045"
ORIGIN      
        1 mqvgalvwar pgsarrwlav vvtasicgqr paapgrlwvc wlaeyrisqe ivarsctspc
       61 adpmawaqat flahnavmaa htagpasmar l
//
LOCUS       XP_077491452             148 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102051 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077491452
VERSION     XP_077491452.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635326.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="uncharacterized protein LOC144102051 isoform X1"
                     /calculated_mol_wt=16759
     CDS             1..148
                     /gene="LOC144102051"
                     /coded_by="XM_077635326.1:354..800"
                     /db_xref="GeneID:144102051"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedrrr rhlnrrltat mkrlsgvhvl
       61 nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar pgevyvvhrc
      121 rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077491457              98 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102051 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077491457
VERSION     XP_077491457.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635331.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..98
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..98
                     /product="uncharacterized protein LOC144102051 isoform X2"
                     /calculated_mol_wt=10604
     CDS             1..98
                     /gene="LOC144102051"
                     /coded_by="XM_077635331.1:433..729"
                     /db_xref="GeneID:144102051"
ORIGIN      
        1 mkrlsgvhvl nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar
       61 pgevyvvhrc rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077491470             412 aa            linear   INV 25-NOV-2025
DEFINITION  xaa-Arg dipeptidase-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077491470
VERSION     XP_077491470.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635344.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..412
                     /product="xaa-Arg dipeptidase-like isoform X1"
                     /calculated_mol_wt=43847
     Region          12..371
                     /region_name="M20_ACY1L2-like"
                     /note="M20 Peptidase aminoacylase 1-like protein 2-like,
                     amidohydrolase subfamily; cd05672"
                     /db_xref="CDD:349921"
     CDS             1..412
                     /gene="LOC144102065"
                     /coded_by="XM_077635344.1:225..1463"
                     /db_xref="GeneID:144102065"
ORIGIN      
        1 meqyrciaqd tidskadalt tlgqylwrnp elafaefkah dyitrylege gfqvvrnyvl
       61 htafraefgc gdvtvavlce ydalpglgha cghnliaqgs vaagvavkea lrrasltgkv
      121 vllgtpaeeg gggkahllka gafdgvhaal mahpstrnva acgttavaky mvrykgrpth
      181 saaspwqgln aldaavaays nvamlrqqlr peyrvsgifr kggdqpniip aeatmefdlr
      241 apgasqledl grrvracfda galssgcevs vkeampsykn lvpnvamakv yrayaeeqgl
      301 rfpdgdvspa ssastdvgnv sqvvptiqpy yaleaatsgg nhtegyrdac ndvrsynitl
      361 svakalalta ikvmtdaell rdmwkefdss hasaqgltrh dgsslpadap ql
//
LOCUS       XP_077491476             386 aa            linear   INV 25-NOV-2025
DEFINITION  xaa-Arg dipeptidase-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077491476
VERSION     XP_077491476.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635350.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..386
                     /product="xaa-Arg dipeptidase-like isoform X2"
                     /calculated_mol_wt=41020
     Region          12..345
                     /region_name="M20_ACY1L2-like"
                     /note="M20 Peptidase aminoacylase 1-like protein 2-like,
                     amidohydrolase subfamily; cd05672"
                     /db_xref="CDD:349921"
     CDS             1..386
                     /gene="LOC144102065"
                     /coded_by="XM_077635350.1:141..1301"
                     /db_xref="GeneID:144102065"
ORIGIN      
        1 meqyrciaqd tidskadalt tlgqylwrnp elafaefkah dyitrylege gfqvvrnyvl
       61 htafraefgc gdvtvavlce ydalpglgha cghnliaqgs vaagvavkea lrrasltgkv
      121 vllgtpaeeg gggkahllka gafdgvhaal mahpstrnva acgttavaky mvrykgrpth
      181 saaspwqgln aldaavaays nvamlrqqlr peyrvsgasq ledlgrrvra cfdagalssg
      241 cevsvkeamp syknlvpnva makvyrayae eqglrfpdgd vspassastd vgnvsqvvpt
      301 iqpyyaleaa tsggnhtegy rdacndvrsy nitlsvakal altaikvmtd aellrdmwke
      361 fdsshasaqg ltrhdgsslp adapql
//
LOCUS       XP_077491483             137 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102075 [Amblyomma americanum].
ACCESSION   XP_077491483
VERSION     XP_077491483.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635357.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..137
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..137
                     /product="uncharacterized protein LOC144102075"
                     /calculated_mol_wt=14467
     CDS             1..137
                     /gene="LOC144102075"
                     /coded_by="XM_077635357.1:85..498"
                     /db_xref="GeneID:144102075"
ORIGIN      
        1 melaagtraa rpagdvqrgh lggpgssslp saslpryrvr thrtrgggtv veakgrltlk
       61 erpheippsr qsrqpsherh awqsqllhlg rlylviqaia iawtaqllks ggrptaprls
      121 vavllllpfp sasgagg
//
LOCUS       XP_077491518             107 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102101 [Amblyomma americanum].
ACCESSION   XP_077491518
VERSION     XP_077491518.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635392.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..107
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..107
                     /product="uncharacterized protein LOC144102101"
                     /calculated_mol_wt=11472
     CDS             1..107
                     /gene="LOC144102101"
                     /coded_by="XM_077635392.1:62..385"
                     /db_xref="GeneID:144102101"
ORIGIN      
        1 mtrnfsfngq wpghmehsvl kicsdpadei kirggrshgc lqlpgepsvd igagtkpstg
       61 tdfalpdtsl alfahspevc viptwvvcga shrhrhcrra ggtkawr
//
LOCUS       XP_077491527             229 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102110 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077491527
VERSION     XP_077491527.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635401.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..229
                     /product="uncharacterized protein LOC144102110 isoform X1"
                     /calculated_mol_wt=25283
     CDS             1..229
                     /gene="LOC144102110"
                     /coded_by="XM_077635401.1:339..1028"
                     /db_xref="GeneID:144102110"
ORIGIN      
        1 mifalvmlvs vllgpacldn eygegednkn eggneggnng eggnedrnkn egvnedgnkd
       61 nhgnqdedgs edaeketaps pcqedscrah ceemfsdpqv nltseclmdd kgeaycsckr
      121 vaycspdmca ascsmyhgsk enltaeceky lckcgwqehp vvqkrshrvq rnvssdgerm
      181 rceedycdee cnkkygyekq vagacdgfnc vcswyigysv hkhplppyg
//
LOCUS       XP_077491534             221 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102110 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077491534
VERSION     XP_077491534.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635408.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..221
                     /product="uncharacterized protein LOC144102110 isoform X2"
                     /calculated_mol_wt=24428
     CDS             1..221
                     /gene="LOC144102110"
                     /coded_by="XM_077635408.1:343..1008"
                     /db_xref="GeneID:144102110"
ORIGIN      
        1 mifalvmlvs vllgpacldn eygegednkn eggneggnng eggnedrnkn egvnedgnkd
       61 nhgnqdedgs edaeketaps pcqedscrah ceemfsdpqv nltseclmdd kgeaycsckr
      121 vasscsmyhg skenltaece kylckcgwqe hpvvqkrshr vqrnvssdge rmrceedycd
      181 eecnkkygye kqvagacdgf ncvcswyigy svhkhplppy g
//
LOCUS       XP_077491541             207 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102110 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077491541
VERSION     XP_077491541.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635415.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..207
                     /product="uncharacterized protein LOC144102110 isoform X3"
                     /calculated_mol_wt=22698
     CDS             1..207
                     /gene="LOC144102110"
                     /coded_by="XM_077635415.1:335..958"
                     /db_xref="GeneID:144102110"
ORIGIN      
        1 mifalvmlvs vllgpacldn eygegednkn eggneggnng eggnedrnkn egvnedgnkd
       61 nhgnqdedgs edaeketaps pcqedscrah ceemfsdpqv nltseclmdd kgeaycsckr
      121 vaycspdmca ascsmyhgsk enltaeceky lckcgwqerc eedycdeecn kkygyekqva
      181 gacdgfncvc swyigysvhk hplppyg
//
LOCUS       XP_077491549             193 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102110 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077491549
VERSION     XP_077491549.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635423.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..193
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..193
                     /product="uncharacterized protein LOC144102110 isoform X4"
                     /calculated_mol_wt=21256
     CDS             1..193
                     /gene="LOC144102110"
                     /coded_by="XM_077635423.1:338..919"
                     /db_xref="GeneID:144102110"
ORIGIN      
        1 mifalvmlvs vllgpacldn eygegednkn eggneggnng eggnedrnkn egvnedgnkd
       61 nhgnqdedgs edaeketaps pcqedscrah ceemfsdpqv nltseclmdd kgeaycsckr
      121 vaypvvqkrs hrvqrnvssd germrceedy cdeecnkkyg yekqvagacd gfncvcswyi
      181 gysvhkhplp pyg
//
LOCUS       XP_077491557             177 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102130 [Amblyomma americanum].
ACCESSION   XP_077491557
VERSION     XP_077491557.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635431.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..177
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..177
                     /product="uncharacterized protein LOC144102130"
                     /calculated_mol_wt=19328
     CDS             1..177
                     /gene="LOC144102130"
                     /coded_by="XM_077635431.1:705..1238"
                     /db_xref="GeneID:144102130"
ORIGIN      
        1 mgrlkstvig ppnadydegl nkdtmgellp gaylqpppka epynsddqql pqestsgssm
       61 slpprqqqll ddakttspsf vspspekvds vpamadadas rwgpaepavp eaetqwrlps
      121 agnslwrlrt rccfmgsvcr rcrwrrlapg aalppslrrp hsglswesql yvlkqra
//
LOCUS       XP_077491571             193 aa            linear   INV 25-NOV-2025
DEFINITION  anaphase-promoting complex subunit 10-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077491571
VERSION     XP_077491571.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635445.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..193
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..193
                     /product="anaphase-promoting complex subunit 10-like
                     isoform X1"
                     /calculated_mol_wt=21326
     Region          22..191
                     /region_name="ANAPC10"
                     /note="Anaphase-promoting complex, subunit 10 (APC10);
                     pfam03256"
                     /db_xref="CDD:367420"
     Site            order(59,86,93,95,158)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176484"
     CDS             1..193
                     /gene="LOC144102137"
                     /coded_by="XM_077635445.1:70..651"
                     /db_xref="GeneID:144102137"
ORIGIN      
        1 maqglptart mnassaqdls tlerqgdvre vgaeavwsls sckpgfgvdq lrdnclntyw
       61 rsdgllphev niqfhrktai qavciyvdye rdesyapkri avkvgstfhd lrvvntvdln
      121 eptgwvhiat qdsagrpvra fhvqiavltn hkdgldtrlr qiklyspvkr asfgvlpgln
      181 ftsaeciafs tir
//
LOCUS       XP_077491577             183 aa            linear   INV 25-NOV-2025
DEFINITION  anaphase-promoting complex subunit 10-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077491577
VERSION     XP_077491577.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635451.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="anaphase-promoting complex subunit 10-like
                     isoform X2"
                     /calculated_mol_wt=20299
     Region          12..181
                     /region_name="ANAPC10"
                     /note="Anaphase-promoting complex, subunit 10 (APC10);
                     pfam03256"
                     /db_xref="CDD:367420"
     Site            order(49,76,83,85,148)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176484"
     CDS             1..183
                     /gene="LOC144102137"
                     /coded_by="XM_077635451.1:167..718"
                     /db_xref="GeneID:144102137"
ORIGIN      
        1 mnassaqdls tlerqgdvre vgaeavwsls sckpgfgvdq lrdnclntyw rsdgllphev
       61 niqfhrktai qavciyvdye rdesyapkri avkvgstfhd lrvvntvdln eptgwvhiat
      121 qdsagrpvra fhvqiavltn hkdgldtrlr qiklyspvkr asfgvlpgln ftsaeciafs
      181 tir
//
LOCUS       XP_077491590             162 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102144 [Amblyomma americanum].
ACCESSION   XP_077491590
VERSION     XP_077491590.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635464.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..162
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..162
                     /product="uncharacterized protein LOC144102144"
                     /calculated_mol_wt=17628
     CDS             1..162
                     /gene="LOC144102144"
                     /coded_by="XM_077635464.1:4..492"
                     /db_xref="GeneID:144102144"
ORIGIN      
        1 mqqvqeyvfv gwrfagmkhv ylileflggr nswvldvipe lrarrgrlqa flcayqrlga
       61 dgpymkllnl aeaattlrkh lglhvaaaya lavledanwl qykgvprdly eeavfakisd
      121 ikrmglsapp kdvpapptad gdehivdgge agepaatpsa ap
//
LOCUS       XP_077491597             162 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102154 [Amblyomma americanum].
ACCESSION   XP_077491597
VERSION     XP_077491597.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635471.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..162
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..162
                     /product="uncharacterized protein LOC144102154"
                     /calculated_mol_wt=17628
     CDS             1..162
                     /gene="LOC144102154"
                     /coded_by="XM_077635471.1:4..492"
                     /db_xref="GeneID:144102154"
ORIGIN      
        1 mqqvqeyvfv gwrfagmkhv ylileflggr nswvldvipe lrarrgrlqa flcayqrlga
       61 dgpymkllnl aeaattlrkh lglhvaaaya lavledanwl qykgvprdly eeavfakisd
      121 ikrmglsapp kdvpapptad gdehivdgge agepaatpsa ap
//
LOCUS       XP_077491608             247 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102164 [Amblyomma americanum].
ACCESSION   XP_077491608
VERSION     XP_077491608.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635482.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..247
                     /product="uncharacterized protein LOC144102164"
                     /calculated_mol_wt=27323
     CDS             1..247
                     /gene="LOC144102164"
                     /coded_by="XM_077635482.1:1..744"
                     /db_xref="GeneID:144102164"
ORIGIN      
        1 mddfylllsl mlcnlprgrg sklgtdwfqn rvndlleffp nvsepprvpl ysveqadais
       61 rfgeqwphtr aalcstllkt rfqgpmqqvq eyvsvgwrfa gmkhvylile flggrnswvl
      121 dvipelrarr grlqaflcay qrlgadgpym kllnlaeaat tlrkhlglhv aaayalavle
      181 danwlqykgv prdlyeeavf akisdikrmg lsappkdvpa pptadgdehi vdggeagepa
      241 atpsaap
//
LOCUS       XP_077491628             402 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102186 [Amblyomma americanum].
ACCESSION   XP_077491628
VERSION     XP_077491628.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635502.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..402
                     /product="uncharacterized protein LOC144102186"
                     /calculated_mol_wt=44365
     CDS             1..402
                     /gene="LOC144102186"
                     /coded_by="XM_077635502.1:45..1253"
                     /db_xref="GeneID:144102186"
ORIGIN      
        1 mfrrrmfrrs srarhsvtas vqgrvlgfpg wcgylvpedr lagrqgvdaa mmpgpmgvva
       61 advlattlpr qmvyvfptlg qfdprcvrli tqdqdpatai acllgffkvv ceagyrhvll
      121 asgldgmwld evhvssafvr rqlrslgadg apeatmddfy lllslmlcnl prgrgsklgt
      181 dwfqnrvndl leffpnvsep prvplyseeq adaisrfceq wphtraalcs tllktrfqgp
      241 mqqvqeyvsv gwrfagmkhv ylileflggr nswvldvipe lrarrgrlqa flcayqrlga
      301 dgpymkllnl aeaattlrkh lglhvaaaya lavledanwl qykgvprdly eeavfakisd
      361 ikrmglsapp kdvpapptad gdehivdgge agepaatpsa ap
//
LOCUS       XP_077491648             183 aa            linear   INV 25-NOV-2025
DEFINITION  anaphase-promoting complex subunit 10-like [Amblyomma americanum].
ACCESSION   XP_077491648
VERSION     XP_077491648.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635522.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="anaphase-promoting complex subunit 10-like"
                     /calculated_mol_wt=20271
     Region          12..181
                     /region_name="ANAPC10"
                     /note="Anaphase-promoting complex, subunit 10 (APC10);
                     pfam03256"
                     /db_xref="CDD:367420"
     Site            order(49,76,83,85,148)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176484"
     CDS             1..183
                     /gene="LOC144102207"
                     /coded_by="XM_077635522.1:151..702"
                     /db_xref="GeneID:144102207"
ORIGIN      
        1 mnassaqdls tlerqgdvre vgaeavwsls sckpgfgvdq lrdnclntyw rsdgllphev
       61 nvqfhrktai qavciyvdye rdesyapkri avkvgstfhd lrvvntvdln eptgwvhiat
      121 qdsagrpvra fhvqiavltn hkdgldtrlr qiklyspvkr asfgvlpgvn ftsaeciafs
      181 tir
//
LOCUS       XP_077491659             247 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102218 [Amblyomma americanum].
ACCESSION   XP_077491659
VERSION     XP_077491659.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635533.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..247
                     /product="uncharacterized protein LOC144102218"
                     /calculated_mol_wt=27323
     CDS             1..247
                     /gene="LOC144102218"
                     /coded_by="XM_077635533.1:16..759"
                     /db_xref="GeneID:144102218"
ORIGIN      
        1 mddfylllsl mlcnlprgrg sklgtdwfqn rvndlleffp nvsepprvpl yseeqadais
       61 rfgeqwphtr aalcstllkt rfqgpmqqvq eyvsvgwrfa gmkhvylile flggrnswvl
      121 dvipelrarr grlqaflcay qrlgadgpym kllnlaeaat tlrkhlglhv aaayalavle
      181 danwlqykgv prdlyeeavf akisdikrmg lgappkdvpa pptadgdehi vdggeagepa
      241 atpsaap
//
LOCUS       XP_077491685             170 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102252 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077491685
VERSION     XP_077491685.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635559.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..170
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..170
                     /product="uncharacterized protein LOC144102252 isoform X1"
                     /calculated_mol_wt=17767
     CDS             1..170
                     /gene="LOC144102252"
                     /coded_by="XM_077635559.1:126..638"
                     /db_xref="GeneID:144102252"
ORIGIN      
        1 masslgatee vpdytvdgwp llneeptsat agdviarpal hihssgprsp sspstsllsg
       61 saipptghsa ltvcgttwlq dknrrcrgkl shgcapmpae pladlaatat ppteawpvay
      121 ssaksrfrap risvsaeaal ltmafawpts ttaaglaahr frrrirplws
//
LOCUS       XP_077491693             170 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102252 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077491693
VERSION     XP_077491693.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635567.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..170
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..170
                     /product="uncharacterized protein LOC144102252 isoform X1"
                     /calculated_mol_wt=17767
     CDS             1..170
                     /gene="LOC144102252"
                     /coded_by="XM_077635567.1:126..638"
                     /db_xref="GeneID:144102252"
ORIGIN      
        1 masslgatee vpdytvdgwp llneeptsat agdviarpal hihssgprsp sspstsllsg
       61 saipptghsa ltvcgttwlq dknrrcrgkl shgcapmpae pladlaatat ppteawpvay
      121 ssaksrfrap risvsaeaal ltmafawpts ttaaglaahr frrrirplws
//
LOCUS       XP_077491700             136 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102252 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077491700
VERSION     XP_077491700.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635574.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..136
                     /product="uncharacterized protein LOC144102252 isoform X2"
                     /calculated_mol_wt=13730
     CDS             1..136
                     /gene="LOC144102252"
                     /coded_by="XM_077635574.1:126..536"
                     /db_xref="GeneID:144102252"
ORIGIN      
        1 masslgatee vpdytvdgwp llneeptsat agdviarpal hihssgprsp sspstsllsg
       61 saipptghsa ltvcgttwlq dknrrcrgkl shgcapmpae pladlaatat tacartsgrl
      121 lgaldkcrgq amagpv
//
LOCUS       XP_077491708             170 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102276 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077491708
VERSION     XP_077491708.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635582.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..170
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..170
                     /product="uncharacterized protein LOC144102276 isoform X1"
                     /calculated_mol_wt=17702
     CDS             1..170
                     /gene="LOC144102276"
                     /coded_by="XM_077635582.1:129..641"
                     /db_xref="GeneID:144102276"
ORIGIN      
        1 masslgatee vpdytvdgwl llneeptsat agdviarpal hidssgprsp sspstsllsg
       61 saipptghsa ltvcgttwlq dknrrcrgkl shgcapmpae pladlaatat ppteawpvay
      121 ssakspfrap risvsaeaal ltmafawpts ttaaglaahr frrrirplws
//
LOCUS       XP_077491715             170 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102276 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077491715
VERSION     XP_077491715.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635589.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..170
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..170
                     /product="uncharacterized protein LOC144102276 isoform X1"
                     /calculated_mol_wt=17702
     CDS             1..170
                     /gene="LOC144102276"
                     /coded_by="XM_077635589.1:129..641"
                     /db_xref="GeneID:144102276"
ORIGIN      
        1 masslgatee vpdytvdgwl llneeptsat agdviarpal hidssgprsp sspstsllsg
       61 saipptghsa ltvcgttwlq dknrrcrgkl shgcapmpae pladlaatat ppteawpvay
      121 ssakspfrap risvsaeaal ltmafawpts ttaaglaahr frrrirplws
//
LOCUS       XP_077491723             136 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102276 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077491723
VERSION     XP_077491723.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635597.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..136
                     /product="uncharacterized protein LOC144102276 isoform X2"
                     /calculated_mol_wt=13724
     CDS             1..136
                     /gene="LOC144102276"
                     /coded_by="XM_077635597.1:129..539"
                     /db_xref="GeneID:144102276"
ORIGIN      
        1 masslgatee vpdytvdgwl llneeptsat agdviarpal hidssgprsp sspstsllsg
       61 saipptghsa ltvcgttwlq dknrrcrgkl shgcapmpae pladlaatat tacartsgrl
      121 lgaldkcrgq amagpv
//
LOCUS       XP_077491734             170 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102301 [Amblyomma americanum].
ACCESSION   XP_077491734
VERSION     XP_077491734.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635608.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..170
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..170
                     /product="uncharacterized protein LOC144102301"
                     /calculated_mol_wt=17752
     CDS             1..170
                     /gene="LOC144102301"
                     /coded_by="XM_077635608.1:126..638"
                     /db_xref="GeneID:144102301"
ORIGIN      
        1 masslgatee vpdytvdgwp llneeptsat agdviarpal hihssgprsp sspstsllsg
       61 saipptahsa ltvcgttwlq dknrrcrgkl shgcapmpae pladlaatat ppteawpvay
      121 ssakspfrap risvsaeaal lmmafawpts ttaaglaahr frrrirplws
//
LOCUS       XP_077491744             146 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102312 [Amblyomma americanum].
ACCESSION   XP_077491744
VERSION     XP_077491744.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635618.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..146
                     /product="uncharacterized protein LOC144102312"
                     /calculated_mol_wt=16002
     CDS             1..146
                     /gene="LOC144102312"
                     /coded_by="XM_077635618.1:285..725"
                     /db_xref="GeneID:144102312"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrppfa altsvlrcqr pasigpcvlp afgcvippdp
       61 fmlktkscme vvqatklrvi fardleqawk lvdgqwysls kasffsktkt iflgswamyl
      121 gtcgqdnagq hasasskkkh etvnky
//
LOCUS       XP_077491769             256 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102347 [Amblyomma americanum].
ACCESSION   XP_077491769
VERSION     XP_077491769.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635643.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..256
                     /product="uncharacterized protein LOC144102347"
                     /calculated_mol_wt=26672
     CDS             1..256
                     /gene="LOC144102347"
                     /coded_by="XM_077635643.1:94..864"
                     /db_xref="GeneID:144102347"
ORIGIN      
        1 madscekgpl pllrqekaal psrgrkkssp rhsgsvrkgs qmavlpaaeq gphsqvaeev
       61 pslpqadkda sywpvsdkql pslpagsatp palpgleerp almssqtepa vlpgiekeal
      121 asgsgkgtpr ssrsgkgsvr stktekdpfp lsnqpstspp qpprgrnssl vkgvavllvl
      181 viallvvmvi lkgakpppkg dvldgnacqs ddckrvarll leslqpnapp cadfyqlvcg
      241 rwkhavssrg agrlvg
//
LOCUS       XP_077491775             587 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102354 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077491775
VERSION     XP_077491775.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635649.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..587
                     /product="uncharacterized protein LOC144102354 isoform X1"
                     /calculated_mol_wt=64158
     Region          361..420
                     /region_name="Kunitz-type"
                     /note="Kunitz/Bovine pancreatic trypsin inhibitor (BPTI)
                     domain; cl00101"
                     /db_xref="CDD:444694"
     Site            order(369..373,375..377,396,403)
                     /site_type="other"
                     /note="putative serine protease binding site [polypeptide
                     binding]"
                     /db_xref="CDD:438633"
     CDS             1..587
                     /gene="LOC144102354"
                     /coded_by="XM_077635649.1:32..1795"
                     /db_xref="GeneID:144102354"
ORIGIN      
        1 masasrrsaq ptslsyasah asqqqpcrrp lpgilkttpg ffsfpkgspt tktlrfqips
       61 ptpgslaqly aqeeaeqdlv latgeaatra kteksdsssp psaeetsydk fhlrghkrrr
      121 spsprrrsss sasgadsvvp ywmpkeiqhy flhypddgee grgaagapct kevpsalqci
      181 gseqpprkde lagwrrlasq prsalatlcf iaalalifvg tttnvyvgmv hrargdgrtp
      241 vvagagagga dsagakkepl gskavprsmg dypdalkrrr aetaeptrrh avaafdskee
      301 reettggdae iegrsrhdev gtdagddlpr pvtmdymgta rdvaeivhef ssneddahrs
      361 paqmrascga lmfsycpspr iefyynpvtn acvstasdga gvcirgpnkf psrqtcrqrc
      421 iedgrpaknc rhraifkect rqdvsqswwy hssnrchrwt fprgrcpasg sdvfttfgec
      481 wrhcvsdeqh snsrakfnel tggwllgqrr gprpcrlprt evcraeslky ayfadssprs
      541 aaagsrglrc hsvpvanqlg hrcleganrf rtakacrrtc vtnsgrh
//
LOCUS       XP_077491780             493 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102354 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077491780
VERSION     XP_077491780.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635654.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..493
                     /product="uncharacterized protein LOC144102354 isoform X2"
                     /calculated_mol_wt=51642
     CDS             1..493
                     /gene="LOC144102354"
                     /coded_by="XM_077635654.1:32..1513"
                     /db_xref="GeneID:144102354"
ORIGIN      
        1 masasrrsaq ptslsyasah asqqqpcrrp lpgilkttpg ffsfpkgspt tktlrfqips
       61 ptpgslaqly aqeeaeqdlv latgeaatra kteksdsssp psaeetsydk fhlrghkrrr
      121 spsprrrsss sasgadsvvp ywmpkeiqhy flhypddgee grgaagapct kevpsalqci
      181 gseqpprkde lagwrrlasq prsalatlcf iaalalifvg tttnvyvgmv hrargdgrtp
      241 vvagagagga dsagakkepl gskavprsmg dypdalkrrr aetaeptrrh avaafdskee
      301 reettggdae iegrsrhdev gtdagddlpr pvtmdymgta rdvaeivhef ssneddahrs
      361 prqmgrasas eaqtssrrgr pagsgasrta dprrtaaiep ssknapgkts lraggitaat
      421 aviggpslgd aalpaavtss rpsgsagaia satsstatav psstsllgdg cwardvapdl
      481 vvsrelrcae rkv
//
LOCUS       XP_077491803             236 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102391 [Amblyomma americanum].
ACCESSION   XP_077491803
VERSION     XP_077491803.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635677.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..236
                     /product="uncharacterized protein LOC144102391"
                     /calculated_mol_wt=27194
     Region          <1..44
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     CDS             1..236
                     /gene="LOC144102391"
                     /coded_by="XM_077635677.1:1..711"
                     /db_xref="GeneID:144102391"
ORIGIN      
        1 meinvsktkt mtftrasnvh lssyfmnsic ienvytlkyl gvhltsnltw ndhideiisk
       61 anktlgfirr nlylanqstk llahtalvrs kieyasliwn pnqtylinkl eslqnkaarf
      121 itktysrtss itaikkslql psletrrlla qlshfhrlyh apssfaasyi kppqkifprl
      181 dhpfkvrpmf artnllrqsp lflaihhwnk lpkeiasird hdsfvsnlkr sfthvg
//
LOCUS       XP_077491811             326 aa            linear   INV 25-NOV-2025
DEFINITION  mortality factor 4-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077491811
VERSION     XP_077491811.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635685.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..326
                     /product="mortality factor 4-like protein 1"
                     /calculated_mol_wt=37781
     Region          11..67
                     /region_name="CBD_MSL3_like"
                     /note="chromo barrel domain of human male-specific lethal
                     complex subunit 3, and similar proteins; cd18983"
                     /db_xref="CDD:350846"
     Site            order(17..18,22..25,40,42..45,49,51..54,56..58,61..62)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:350846"
     Site            order(22,42,45,49)
                     /site_type="other"
                     /note="putative methylated histone tail binding site
                     [chemical binding]"
                     /db_xref="CDD:350846"
     Region          24..>117
                     /region_name="SAS2"
                     /note="Histone acetyltransferase (MYST family) [Chromatin
                     structure and dynamics]; COG5027"
                     /db_xref="CDD:227360"
     Site            order(39,41..46,48..52)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:350846"
     Site            order(39,42,46,48,52)
                     /site_type="other"
                     /note="putative RNA binding site [nucleotide binding]"
                     /db_xref="CDD:350846"
     Region          141..314
                     /region_name="MRG"
                     /note="pfam05712"
                     /db_xref="CDD:461721"
     CDS             1..326
                     /gene="LOC144102402"
                     /coded_by="XM_077635685.1:49..1029"
                     /db_xref="GeneID:144102402"
ORIGIN      
        1 mdltsrfqed ekvlcfdgpl iyeakclkvr frnkkaqyfv hyigwnkrwd ewipesrmlk
       61 yddinlqkkk eldeaykgmk kkkpepepee ekdlddssvs seekpqkkka tgtrvassva
      121 snqlsttraa esgggrkrsh rdthvepget fllgeefkvr ipdelkpwll ddrylvtqhk
      181 mlvqipcsvt vdqiladylt ektseantss hqaritertd cfkkyfnmml gsqllykfer
      241 pqyadileer rgtpmsqiyg aihllrlfvr ldsmlktvsl ekpgfqncva aiedllryma
      301 enskklftld gydtatpeyi rrtsss
//
LOCUS       XP_077491838              91 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102437 [Amblyomma americanum].
ACCESSION   XP_077491838
VERSION     XP_077491838.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635712.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..91
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..91
                     /product="uncharacterized protein LOC144102437"
                     /calculated_mol_wt=9044
     CDS             1..91
                     /gene="LOC144102437"
                     /coded_by="XM_077635712.1:122..397"
                     /db_xref="GeneID:144102437"
ORIGIN      
        1 merkrhlgtr tqpqraapmq aiaatassar sdpeagspeg tlgalgqpcn cseiaatsct
       61 spcadpmawa qatflahnaa maahtagpva a
//
LOCUS       XP_077491847             105 aa            linear   INV 25-NOV-2025
DEFINITION  DNA (cytosine-5)-methyltransferase PliMCI-like [Amblyomma
            americanum].
ACCESSION   XP_077491847
VERSION     XP_077491847.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635721.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..105
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..105
                     /product="DNA (cytosine-5)-methyltransferase PliMCI-like"
                     /calculated_mol_wt=11892
     Region          <7..>82
                     /region_name="Dcm"
                     /note="DNA-cytosine methylase [Replication, recombination
                     and repair]; COG0270"
                     /db_xref="CDD:440040"
     CDS             1..105
                     /gene="LOC144102450"
                     /coded_by="XM_077635721.1:19..336"
                     /db_xref="GeneID:144102450"
ORIGIN      
        1 mmfsmlqnsl vctylsccdy yrpqhfllen vqgflyrleg kilpltmkcl lrmgyqctfa
       61 rlqagnygvp qsrlrwshef litaqpglrt rlsgvgksca gsfpl
//
LOCUS       XP_077491874             164 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102482 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077491874
VERSION     XP_077491874.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635748.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..164
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..164
                     /product="uncharacterized protein LOC144102482 isoform X1"
                     /calculated_mol_wt=17308
     CDS             1..164
                     /gene="LOC144102482"
                     /coded_by="XM_077635748.1:164..658"
                     /db_xref="GeneID:144102482"
ORIGIN      
        1 mppiavqapq rrsfgvpvsc trlrlqaaed hflsrhhata lateivctpl atvsqaepts
       61 cpsfarrkrs pipapprqvg ppaaaaaasr rrgalldgrg afrpwidrgs hraagrahgl
      121 rgfgsgdllh tplgrlysvt htdsfqaagi sigllcfdgv idhl
//
LOCUS       XP_077491881             110 aa            linear   INV 25-NOV-2025
DEFINITION  high affinity choline transporter 1-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077491881
VERSION     XP_077491881.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635755.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..110
                     /product="high affinity choline transporter 1-like isoform
                     X2"
                     /calculated_mol_wt=11918
     Region          <46..>98
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     CDS             1..110
                     /gene="LOC144102482"
                     /coded_by="XM_077635755.1:164..496"
                     /db_xref="GeneID:144102482"
ORIGIN      
        1 mppiavqapq rrsfgvpvsc trlrlqaaed hflsrhhata lateirrhgk wvrllllppp
       61 avggelfwta avlsalgstv evivqldarm afvvsapvtf firplddstp
//
LOCUS       XP_077491886             110 aa            linear   INV 25-NOV-2025
DEFINITION  high affinity choline transporter 1-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077491886
VERSION     XP_077491886.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635760.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..110
                     /product="high affinity choline transporter 1-like isoform
                     X2"
                     /calculated_mol_wt=11918
     Region          <46..>98
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     CDS             1..110
                     /gene="LOC144102482"
                     /coded_by="XM_077635760.1:164..496"
                     /db_xref="GeneID:144102482"
ORIGIN      
        1 mppiavqapq rrsfgvpvsc trlrlqaaed hflsrhhata lateirrhgk wvrllllppp
       61 avggelfwta avlsalgstv evivqldarm afvvsapvtf firplddstp
//
LOCUS       XP_077491889             936 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102502 [Amblyomma americanum].
ACCESSION   XP_077491889
VERSION     XP_077491889.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635763.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..936
                     /product="uncharacterized protein LOC144102502"
                     /calculated_mol_wt=105040
     Region          13..33
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(13,16,29,33)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(18,20,22,24..25,28..29,32,46,52..53,56..57,61,75,77,
                     79,81..82,85..86)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          41..62
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          70..86
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          191..211
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(191,194,207,211)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          221..242
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(221,224,237,242)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(226,228,230,232..233,236..237,240,272,274,278..279,
                     282..283,286,302,304,306,308..309,312..313,316)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          267..287
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          297..317
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          381..401
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(381,384,397,401)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          409..429
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          495..515
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(495,498,511,515)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          523..543
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(523,526,539,543)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(528,530,532,534..535,538..539,542,564,566,570..571,
                     574..575,578,592,594,596,598..599,602..603,606)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          559..579
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          587..607
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..936
                     /gene="LOC144102502"
                     /coded_by="XM_077635763.1:170..2980"
                     /db_xref="GeneID:144102502"
ORIGIN      
        1 mkpgcltips yrcksctfvg nnrkhlanhv alnhssrvyr cslcvfvtry ksnfyrhrrn
       61 ihklnsanvc emcgqacdnq delvahasne hpqfldallr kleakgvrct ryaakmnmed
      121 ggtahasakk tvaknlqpel vtgdvsgqiy lqngvpekqn enaeqskkrp ressppvsnk
      181 kgrtgrltyt caecdfvtkw preflshrrd vhgdrisihe cpfceyaskq lqnlkkhcnt
      241 vhkeemyggi rtmttmvngt ahrmfkcrfc pltnkrranv kihermhtas kgakfqcvlc
      301 syrcnntgvl arhmklhkad npsldlsrmk tfcenlnalp srpavakksv pesaaaqrse
      361 isalkdklkt psrkkifsyf cdkcpamfks htdldthkty hnsahlypch lcdyrarrkp
      421 hlhkhllvht pkyaerqngl tlaeirsqdr sqqpesatpp aapaaadqml llekaetraf
      481 vdagsqsmav klhychlcpg aflnartlky hlslhgtaav yycpfctyaa nstanmsahk
      541 rlhylnvlkq kqppkmfrcn kcpasfsryn rfdshltlhg snerfrcsqc dysvkfaanl
      601 vkhrklheil vpeskqlkve ppapltstph ppengsgptt avplsdsapn aatpavveek
      661 hvyicskcpy afhrretvvn hlqrhgtseg lcctfcdyrs vhmstlgdht rrhfqpvchy
      721 kpqaymkcds leiwstssdg trtvvfrddg dekyfpeldd cddageddts spastpassg
      781 scsastascs pqlqlnnkdl epavlklvkh rtddvvlask etleelvsnv vmlndkkdem
      841 ppgcktqvvn tavldgtkql nevgevaqae hksesetdst ddgesaqdlf ncilnpapme
      901 cedledekdv yyetisevpd vayevecetv eedliv
//
LOCUS       XP_077491895             137 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102509 [Amblyomma americanum].
ACCESSION   XP_077491895
VERSION     XP_077491895.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635769.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..137
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..137
                     /product="uncharacterized protein LOC144102509"
                     /calculated_mol_wt=15418
     CDS             1..137
                     /gene="LOC144102509"
                     /coded_by="XM_077635769.1:70..483"
                     /db_xref="GeneID:144102509"
ORIGIN      
        1 maqkawgpqf rqtlfrctms qsalsaslsq qprtaphglp llsllqlwmq trekqgaphm
       61 vvqtnqpihe hllhpqtgph ssrplphlql qwlqrvearv trhdcsptcc pcpelclhsl
      121 agtrklevtd llwksvn
//
LOCUS       XP_077491902             464 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102519 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077491902
VERSION     XP_077491902.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635776.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..464
                     /product="uncharacterized protein LOC144102519 isoform X1"
                     /calculated_mol_wt=51113
     CDS             1..464
                     /gene="LOC144102519"
                     /coded_by="XM_077635776.1:47..1441"
                     /db_xref="GeneID:144102519"
ORIGIN      
        1 mdedsifwri frcflrneyy fnrrriyyld ggdpgenega slredvfgtp ggttqrdera
       61 aslgavqsge lprgprgagr apepsneygr lirpnllrgp aichrcflae hlryynmvkr
      121 rmgrqeaask aegppaaaap angtrkttde sqstnvdnqe kykegqqeqq qqqhqrqqqh
      181 qqqqqqqlkq qqqlkqqqqq qqqlkqqqqq qqqqqlkqqq qqqqqqqqqq qqqlkqqqqq
      241 qqqqqqqqqq qqqywaksld ltsffaslni skiftatpke adksaggrgt dggtaggtpg
      301 qgsadahghs arhsrcgavt spcargagcv akrgavgcnr rvrvlklevp vrlrrgtagp
      361 sgacapaeaa anianreplr nsvvedggpa srlrsktgva kteaadstdr tavggrgarr
      421 vdaqgaadhp degsatargg strqrpstrs trtaaprasg rrpr
//
LOCUS       XP_077491906             423 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102519 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077491906
VERSION     XP_077491906.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635780.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..423
                     /product="uncharacterized protein LOC144102519 isoform X2"
                     /calculated_mol_wt=46977
     CDS             1..423
                     /gene="LOC144102519"
                     /coded_by="XM_077635780.1:47..1318"
                     /db_xref="GeneID:144102519"
ORIGIN      
        1 mdedsifwri frcflrneyy fnrrriyyld ggdpgengra pepsneygrl irpnllrgpa
       61 ichrcflaeh lryynmvkrr mgrqeaaska egppaaaapa ngtrkttdes qstnvdnqek
      121 ykegqqeqqq qqhqrqqqhq qqqqqqlkqq qqlkqqqqqq qqlkqqqqqq qqqqlkqqqq
      181 qqqqqqqqqq qqlkqqqqqq qqqqqqqqqq qqywaksldl tsffaslnis kiftatpkea
      241 dksaggrgtd ggtaggtpgq gsadahghsa rhsrcgavts pcargagcva krgavgcnrr
      301 vrvlklevpv rlrrgtagps gacapaeaaa nianreplrn svvedggpas rlrsktgvak
      361 teaadstdrt avggrgarrv daqgaadhpd egsatarggs trqrpstrst rtaaprasgr
      421 rpr
//
LOCUS       XP_077491909             454 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102531 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077491909
VERSION     XP_077491909.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635783.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..454
                     /product="uncharacterized protein LOC144102531 isoform X1"
                     /calculated_mol_wt=49438
     Region          <326..>453
                     /region_name="PRK07003"
                     /note="DNA polymerase III subunit gamma/tau"
                     /db_xref="CDD:235906"
     CDS             1..454
                     /gene="LOC144102531"
                     /coded_by="XM_077635783.1:14..1378"
                     /db_xref="GeneID:144102531"
ORIGIN      
        1 maalqrllwt clrqrlfalr rdlsdldtnd gegpggqesd dppaqaaaeq attrqpnlvp
       61 sgegsaapat dtscirshpa svnplrdaal chhcfrayhl qyysrlrrrm rlqaavqegs
      121 staavtaatl acttgnaghk edkeeegeqq qqkkknkkrq qqqqqqqqqq qqqqqqqqqq
      181 qqpqqqlqqh qhqeqqeqqq kdvaksldls sffstlkiar vftatnqpte srdveketad
      241 paqdygnrrd strehniahr lctaeslpcs rgkkcvarlg psgctripva rprlllrphr
      301 eserptttiq rrngepraip cdeggpatrl rsrnrnqaap sapggtgrqg eaalgavrpa
      361 esrahepaec rasrtarggq aadpksagqt aparatgsat vraregrrvh aqgaagktda
      421 ddssvaatpg ssrqgprtrs tkasapgapv rrpr
//
LOCUS       XP_077491916             410 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102531 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077491916
VERSION     XP_077491916.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635790.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..410
                     /product="uncharacterized protein LOC144102531 isoform X2"
                     /calculated_mol_wt=45052
     CDS             1..410
                     /gene="LOC144102531"
                     /coded_by="XM_077635790.1:14..1246"
                     /db_xref="GeneID:144102531"
ORIGIN      
        1 maalqrllwt clrqrlfalr rdlsdldtnd gegpggqesd dppaqaaaeq attrqpnlvp
       61 sgegsaapat dtscirshpa svnplrdaal chhcfrayhl qyysrlrrrm rlqaavqegs
      121 staavtaatl acttgnaghk edkeeegeqq qqkkknkkrq qqqqqqqqqq qqqqqqqqqq
      181 qqpqqqlqqh qhqeqqeqqq kdvaksldls sffstlkiar vftatnqpte srdveketad
      241 paqdygnrrd strehniahr lctaeslpcs rgkkcvarlg psgctripva rprlllrphr
      301 eserptttiq rrngepraip cdeggpatrl rsrnrnqaap sapggtgrqg eaalgavrar
      361 egrrvhaqga agktdaddss vaatpgssrq gprtrstkas apgapvrrpr
//
LOCUS       XP_077491930             119 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102563 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077491930
VERSION     XP_077491930.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635804.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..119
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..119
                     /product="uncharacterized protein LOC144102563 isoform X1"
                     /calculated_mol_wt=13152
     CDS             1..119
                     /gene="LOC144102563"
                     /coded_by="XM_077635804.1:24..383"
                     /db_xref="GeneID:144102563"
ORIGIN      
        1 mtsttlsnrc flsaglklkg ddaslalrqi ypdwisptit rkgsaqprdt rvamqfslsr
       61 rymvgvglhs tkmlfrllcw ildadssaav calmdeisvl tsfglmrcef ettcsykqv
//
LOCUS       XP_077491936              66 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102563 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077491936
VERSION     XP_077491936.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635810.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..66
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..66
                     /product="uncharacterized protein LOC144102563 isoform X2"
                     /calculated_mol_wt=7361
     CDS             1..66
                     /gene="LOC144102563"
                     /coded_by="XM_077635810.1:170..370"
                     /db_xref="GeneID:144102563"
ORIGIN      
        1 mqfslsrrym vgvglhstkm lfrllcwild adssaavcal mdeisvltsf glmrcefett
       61 csykqv
//
LOCUS       XP_077491947             119 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102589 [Amblyomma americanum].
ACCESSION   XP_077491947
VERSION     XP_077491947.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635821.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..119
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..119
                     /product="uncharacterized protein LOC144102589"
                     /calculated_mol_wt=13192
     CDS             1..119
                     /gene="LOC144102589"
                     /coded_by="XM_077635821.1:63..422"
                     /db_xref="GeneID:144102589"
ORIGIN      
        1 mtstilsnrc flsaalklka ddaslalrqi ypdwisptit rkgsaqprdt rvamqfslsr
       61 rymvgvglhs tkmlfrllcw ildadssaav calmdeisvl tsfglmrcef ettcsykqv
//
LOCUS       XP_077491955             246 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102597 [Amblyomma americanum].
ACCESSION   XP_077491955
VERSION     XP_077491955.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635829.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..246
                     /product="uncharacterized protein LOC144102597"
                     /calculated_mol_wt=28068
     CDS             1..246
                     /gene="LOC144102597"
                     /coded_by="XM_077635829.1:1..741"
                     /db_xref="GeneID:144102597"
ORIGIN      
        1 mqlflkkfvi evnttevvkw tylttthysk pavlccsvda paraallnmv pfngyfgcpw
       61 cfvrgehveg smryiikeqp earttymvir dmklaegydn iingfkgpsa lmnlggldlv
      121 hsvligvvrq vteiilstsn sgqhfyvgrp stledidsrl laikpplcvt rlprpirerg
      181 hwkasewrqw ilfyalpcle gilhpdywrh lcklsealhi llreeltlse iakaellles
      241 fyiqck
//
LOCUS       XP_077491959             327 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102605 [Amblyomma americanum].
ACCESSION   XP_077491959
VERSION     XP_077491959.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635833.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..327
                     /product="uncharacterized protein LOC144102605"
                     /calculated_mol_wt=35026
     CDS             1..327
                     /gene="LOC144102605"
                     /coded_by="XM_077635833.1:76..1059"
                     /db_xref="GeneID:144102605"
ORIGIN      
        1 mssrtmdvas aqlkpdgsip epavgseegg vreivqstsq gnaevqdlll ieysvlyicn
       61 vcrnlfcsva evlvhkrvyw trhecggmah vsgdrdqplq rqtgvasgac ssrgrdedaa
      121 vseasvsvhr sgqegqgsps hrdfpsgwsp pvirsrssps rgfssssrgs pllrnpaysn
      181 rdssssgrgl qryptpssqd sssdyecsmh qcrfpgrats phrdqpspag fvsgarrlsa
      241 rqsgqrpdgh agngtqpatp vgrnlrsnvr rlvsagggaq pgqqaaqrtq rrrvlqasrd
      301 rvitrsqsqr rvsaplslra akrvgpr
//
LOCUS       XP_077491970             307 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102620 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077491970
VERSION     XP_077491970.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635844.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..307
                     /product="uncharacterized protein LOC144102620 isoform X1"
                     /calculated_mol_wt=32232
     Region          32..>112
                     /region_name="APC10-like"
                     /note="APC10-like DOC1 domains in E3 ubiquitin ligases
                     that mediate substrate ubiquitination; cl02148"
                     /db_xref="CDD:382862"
     Site            order(72,99,106,108)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176482"
     CDS             1..307
                     /gene="LOC144102620"
                     /coded_by="XM_077635844.1:347..1270"
                     /db_xref="GeneID:144102620"
ORIGIN      
        1 mqglliwtsg arrhfvqpcs adtmssgyvr hpsrsetkgr vpeagaqavw sassqqpvyg
       61 vgrlrnncld arwqssglql plisiqfhck vtiqgiyica dymrgescnp nrrfgsvhrh
      121 sqpqatairr dvapeptppv hfccpasqsp aspppspqgq ensevtcadd ttpkaagrad
      181 tdhgcfevlt srppvglqps ditgavtplc sliqqhqaaf vahtsearkl vdecaaanvs
      241 tlsllqstvq glqeqqtaqm atglnimglt sptagavgvs sssggvgqch hisaifissp
      301 sadpglg
//
LOCUS       XP_077491974             285 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102620 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077491974
VERSION     XP_077491974.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635848.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..285
                     /product="uncharacterized protein LOC144102620 isoform X2"
                     /calculated_mol_wt=30727
     Region          32..>112
                     /region_name="APC10-like"
                     /note="APC10-like DOC1 domains in E3 ubiquitin ligases
                     that mediate substrate ubiquitination; cl02148"
                     /db_xref="CDD:382862"
     Site            order(72,99,106,108)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176482"
     CDS             1..285
                     /gene="LOC144102620"
                     /coded_by="XM_077635848.1:343..1200"
                     /db_xref="GeneID:144102620"
ORIGIN      
        1 mqglliwtsg arrhfvqpcs adtmssgyvr hpsrsetkgr vpeagaqavw sassqqpvyg
       61 vgrlrnncld arwqssglql plisiqfhck vtiqgiyica dymrgescnp nrrfgsvhrh
      121 sqpqatairr dvapeptppv hfccpasqsp aspppspqgq ensevtcadd ttpkaagrad
      181 tdhgcfevlt srppvglqps ditgavtplc sliqqhqaaf vahtsearkl vdecaaanvs
      241 tlsllqstvq glqeqqtaqm atgllqrrcg tvpphlryfh llpic
//
LOCUS       XP_077491982             284 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102620 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077491982
VERSION     XP_077491982.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635856.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..284
                     /product="uncharacterized protein LOC144102620 isoform X3"
                     /calculated_mol_wt=29675
     Region          9..>89
                     /region_name="APC10-like"
                     /note="APC10-like DOC1 domains in E3 ubiquitin ligases
                     that mediate substrate ubiquitination; cl02148"
                     /db_xref="CDD:382862"
     Site            order(49,76,83,85)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176482"
     CDS             1..284
                     /gene="LOC144102620"
                     /coded_by="XM_077635856.1:405..1259"
                     /db_xref="GeneID:144102620"
ORIGIN      
        1 mssgyvrhps rsetkgrvpe agaqavwsas sqqpvygvgr lrnncldarw qssglqlpli
       61 siqfhckvti qgiyicadym rgescnpnrr fgsvhrhsqp qatairrdva peptppvhfc
      121 cpasqspasp ppspqgqens evtcaddttp kaagradtdh gcfevltsrp pvglqpsdit
      181 gavtplcsli qqhqaafvah tsearklvde caaanvstls llqstvqglq eqqtaqmatg
      241 lnimgltspt agavgvssss ggvgqchhis aifisspsad pglg
//
LOCUS       XP_077491995              88 aa            linear   INV 25-NOV-2025
DEFINITION  anaphase-promoting complex subunit 10-like [Amblyomma americanum].
ACCESSION   XP_077491995
VERSION     XP_077491995.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635869.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..88
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..88
                     /product="anaphase-promoting complex subunit 10-like"
                     /calculated_mol_wt=9534
     Region          20..>75
                     /region_name="APC10-like"
                     /note="APC10-like DOC1 domains in E3 ubiquitin ligases
                     that mediate substrate ubiquitination; cl02148"
                     /db_xref="CDD:382862"
     CDS             1..88
                     /gene="LOC144102651"
                     /coded_by="XM_077635869.1:403..669"
                     /db_xref="GeneID:144102651"
ORIGIN      
        1 mssgyvrhps rsetkgrvpe agaqavwsas sqqpvygvgr lrnnwldarw qssglqlcpi
       61 sihfhrkais yariysecla svgsetaa
//
LOCUS       XP_077492025             840 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102705 [Amblyomma americanum].
ACCESSION   XP_077492025
VERSION     XP_077492025.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635899.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..840
                     /product="uncharacterized protein LOC144102705"
                     /calculated_mol_wt=92081
     Region          65..>271
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..840
                     /gene="LOC144102705"
                     /coded_by="XM_077635899.1:405..2927"
                     /db_xref="GeneID:144102705"
ORIGIN      
        1 mpllrlsaar alrendslrf nsccpfcifv nssvacgfvc epamerrtgq kyrtkyaygk
       61 ckrkppipkq krpaatephe rrsapissha dvsseaslpl esatssesgg lapsashdia
      121 vqyrpghssv faaasggavp vqwddgevlp kqkhtvqvhl psesitgses vgpapstsrd
      181 iavqyrpghs svfaaasaga vpvqrddgev fpkqkrtvqv hlpsesmtgs esgdpapsts
      241 rdiavqhrpg qssvfaaasd dampaerdds elfpkqkrtv qvhleprfvt leaaeeqacs
      301 vratlravss tdckfgfarq teeesapded eftliqasvm nsiigsllcp kcskkrlsvk
      361 hetklglavk lvlscgacgp iashwsskrk segrtfevni ramqaiktig kwptalndfw
      421 atmnvshlgl hqktyqghlk klfkpgaeea aqnifadavl avkdvyskmq psspsnitvv
      481 ydgtwltrgh sshigvgcii efytglvldc tvlsnfclgc cqqpaesdps ygtwieqhqc
      541 qkntdvasgq meveaalilf rrsfssyglr ytnviwdgds rtylalckde vygfipltke
      601 dcinhvqkrm gtalctlvar akkgeplggk ggltqdlikk ltnyyglalr rntkvsdmqr
      661 avmatlyhvt stddephhel cppgpdswcr hrsaqakmep plphkyklpr rvaeallpvy
      721 qrlsdpqlle rckgnktqna aeslhsviws miskdqhasl favetavhea varynfgnlr
      781 aytevcksvg ikpgslaler aqekdkqrkr kacsaektke qrhkktpask dtkyyspgaf
//
LOCUS       XP_077492033              94 aa            linear   INV 25-NOV-2025
DEFINITION  importin-7-like [Amblyomma americanum].
ACCESSION   XP_077492033
VERSION     XP_077492033.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635907.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..94
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..94
                     /product="importin-7-like"
                     /calculated_mol_wt=10093
     CDS             1..94
                     /gene="LOC144102715"
                     /coded_by="XM_077635907.1:221..505"
                     /db_xref="GeneID:144102715"
ORIGIN      
        1 mwfgaltsgp qsveqnksaq evrvlagqkk aavetrriee sggyvfqnqt vpppissggm
       61 hfcgthdiyq qkastscltm esrfdkrlta sgad
//
LOCUS       XP_077492042              96 aa            linear   INV 25-NOV-2025
DEFINITION  importin-7-like [Amblyomma americanum].
ACCESSION   XP_077492042
VERSION     XP_077492042.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635916.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..96
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..96
                     /product="importin-7-like"
                     /calculated_mol_wt=10395
     CDS             1..96
                     /gene="LOC144102726"
                     /coded_by="XM_077635916.1:143..433"
                     /db_xref="GeneID:144102726"
ORIGIN      
        1 mesttaslhe eeclvdeypv pkeimrgiqn tdpmwfgalt sgpqsveqsr stqeanvqag
       61 qrkaaaesnr meksggyvfq kqtvppsfss ggtpfr
//
LOCUS       XP_077492050             160 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102737 [Amblyomma americanum].
ACCESSION   XP_077492050
VERSION     XP_077492050.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635924.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..160
                     /product="uncharacterized protein LOC144102737"
                     /calculated_mol_wt=17001
     CDS             1..160
                     /gene="LOC144102737"
                     /coded_by="XM_077635924.1:79..561"
                     /db_xref="GeneID:144102737"
ORIGIN      
        1 mkktyadnhk pttaiagaea vaskqstntr kpsenatnpr kaatgagdly sppstrsqkd
       61 rckkemekve dsmseaskps kpkfdadtgr sdlkelrsap rakgarsaap kkvptsrqdr
      121 arphldtsaq gtsiasmrak gpqkallkap sktgnsvrlk
//
LOCUS       XP_077492067              94 aa            linear   INV 25-NOV-2025
DEFINITION  importin-7-like [Amblyomma americanum].
ACCESSION   XP_077492067
VERSION     XP_077492067.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635941.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..94
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..94
                     /product="importin-7-like"
                     /calculated_mol_wt=10093
     CDS             1..94
                     /gene="LOC144102766"
                     /coded_by="XM_077635941.1:221..505"
                     /db_xref="GeneID:144102766"
ORIGIN      
        1 mwfgaltsgp qsveqnksaq evrvlagqkk aavetrriee sggyvfqnqt vpppissggm
       61 hfcgthdiyq qkastscltm esrfdkrlta sgad
//
LOCUS       XP_077492080             187 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102791 [Amblyomma americanum].
ACCESSION   XP_077492080
VERSION     XP_077492080.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635954.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="uncharacterized protein LOC144102791"
                     /calculated_mol_wt=20021
     CDS             1..187
                     /gene="LOC144102791"
                     /coded_by="XM_077635954.1:263..826"
                     /db_xref="GeneID:144102791"
ORIGIN      
        1 mivlhyrnyg lssideynyr gslckfgvgl tlafigltvc fshlvagvvc lscglvilai
       61 pmqylsraka spssptpfps rspllemrpp tdrpppaatv pspveppdrf lqvlstpped
      121 dedggmygdv gkvdsaslvh qyidrvgsls terepswhsl ylslsedhdq ttapaasgee
      181 lqtaavs
//
LOCUS       XP_077492085             187 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102791 [Amblyomma americanum].
ACCESSION   XP_077492085
VERSION     XP_077492085.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635959.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="uncharacterized protein LOC144102791"
                     /calculated_mol_wt=20021
     CDS             1..187
                     /gene="LOC144102791"
                     /coded_by="XM_077635959.1:373..936"
                     /db_xref="GeneID:144102791"
ORIGIN      
        1 mivlhyrnyg lssideynyr gslckfgvgl tlafigltvc fshlvagvvc lscglvilai
       61 pmqylsraka spssptpfps rspllemrpp tdrpppaatv pspveppdrf lqvlstpped
      121 dedggmygdv gkvdsaslvh qyidrvgsls terepswhsl ylslsedhdq ttapaasgee
      181 lqtaavs
//
LOCUS       XP_077492090             187 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102791 [Amblyomma americanum].
ACCESSION   XP_077492090
VERSION     XP_077492090.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635964.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="uncharacterized protein LOC144102791"
                     /calculated_mol_wt=20021
     CDS             1..187
                     /gene="LOC144102791"
                     /coded_by="XM_077635964.1:437..1000"
                     /db_xref="GeneID:144102791"
ORIGIN      
        1 mivlhyrnyg lssideynyr gslckfgvgl tlafigltvc fshlvagvvc lscglvilai
       61 pmqylsraka spssptpfps rspllemrpp tdrpppaatv pspveppdrf lqvlstpped
      121 dedggmygdv gkvdsaslvh qyidrvgsls terepswhsl ylslsedhdq ttapaasgee
      181 lqtaavs
//
LOCUS       XP_077492094             187 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102791 [Amblyomma americanum].
ACCESSION   XP_077492094
VERSION     XP_077492094.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635968.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="uncharacterized protein LOC144102791"
                     /calculated_mol_wt=20021
     CDS             1..187
                     /gene="LOC144102791"
                     /coded_by="XM_077635968.1:373..936"
                     /db_xref="GeneID:144102791"
ORIGIN      
        1 mivlhyrnyg lssideynyr gslckfgvgl tlafigltvc fshlvagvvc lscglvilai
       61 pmqylsraka spssptpfps rspllemrpp tdrpppaatv pspveppdrf lqvlstpped
      121 dedggmygdv gkvdsaslvh qyidrvgsls terepswhsl ylslsedhdq ttapaasgee
      181 lqtaavs
//
LOCUS       XP_077492097             256 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102946 [Amblyomma americanum].
ACCESSION   XP_077492097
VERSION     XP_077492097.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635971.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..256
                     /product="uncharacterized protein LOC144102946"
                     /calculated_mol_wt=28543
     Region          183..>232
                     /region_name="BEN"
                     /note="The BEN domain is found in diverse animal proteins;
                     cl11158"
                     /db_xref="CDD:472128"
     CDS             1..256
                     /gene="LOC144102946"
                     /coded_by="XM_077635971.1:260..1030"
                     /db_xref="GeneID:144102946"
ORIGIN      
        1 mdhilikwtq eekwdvypir amvntavgfr lltepgaidd vrgtielftw kdseppapae
       61 vldigkarvl etkrarlasa glrdegdvmt eslecspnee fcsqcenmrr eldvvkrrne
      121 dleaahesqk vlkklkkmld dmksidaisa vrpcpqidig ggvlieetal eklrsscpga
      181 pakfarglmr qlftadelrg kslfgrksns hpdaslreal dpvrvnaiig ftirefkadp
      241 irlksslssl lsreik
//
LOCUS       XP_077492098             240 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102962 [Amblyomma americanum].
ACCESSION   XP_077492098
VERSION     XP_077492098.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635972.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..240
                     /product="uncharacterized protein LOC144102962"
                     /calculated_mol_wt=25748
     CDS             1..240
                     /gene="LOC144102962"
                     /coded_by="XM_077635972.1:72..794"
                     /db_xref="GeneID:144102962"
ORIGIN      
        1 mdsgryssat estdedtrgr kkapqppssp qysegghsss asqgsgtgkn hspplgpgae
       61 vedfdsfded llldallqea alhnlrpmpg vaevhpvvat keshrddrsd asspastvsl
      121 pssvivsdsg yladedptpe seqdnmsevq rrlvtvlgml regknieshe tcpehcfcpr
      181 stqyiqtare aislsaflay hgeplprnsp iedpeiamll eillrhregr dtneggngsa
//
LOCUS       XP_077492099             240 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102978 [Amblyomma americanum].
ACCESSION   XP_077492099
VERSION     XP_077492099.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635973.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..240
                     /product="uncharacterized protein LOC144102978"
                     /calculated_mol_wt=25815
     CDS             1..240
                     /gene="LOC144102978"
                     /coded_by="XM_077635973.1:103..825"
                     /db_xref="GeneID:144102978"
ORIGIN      
        1 mdsgryssat estdedtrgr kkapqppssp qysegghsss asqgsgtgkn hspplgpgae
       61 vedfdsfded llldallqea alhnlrpmpg vaevypvvat keahrddrsd asspastvsl
      121 pssvivsdsg yladedptpe seqdnmsevq rrlvtvlgml regknieshe tcpehcfcpr
      181 stqyiqtare aislsaflay hgeplprisp iedpeiamll eillrhregr drnesgsgsa
//
LOCUS       XP_077492100             107 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144102994 [Amblyomma americanum].
ACCESSION   XP_077492100
VERSION     XP_077492100.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635974.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..107
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..107
                     /product="uncharacterized protein LOC144102994"
                     /calculated_mol_wt=11124
     CDS             1..107
                     /gene="LOC144102994"
                     /coded_by="XM_077635974.1:340..663"
                     /db_xref="GeneID:144102994"
ORIGIN      
        1 mdvaesqetl agpsqqasmd vaesqetlag psqqapmdvt esqetlpgps qqasmdvatm
       61 ldiqpgpsqa fqltpelptl ptapasvgtp rrrrpamvng pvkcayf
//
LOCUS       XP_077492101             507 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103021 [Amblyomma americanum].
ACCESSION   XP_077492101
VERSION     XP_077492101.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635975.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..507
                     /product="uncharacterized protein LOC144103021"
                     /calculated_mol_wt=56814
     CDS             1..507
                     /gene="LOC144103021"
                     /coded_by="XM_077635975.1:22..1545"
                     /db_xref="GeneID:144103021"
ORIGIN      
        1 mmqsllsrqk nvvhilpver inaqqlhtil hsiitqlehv glriiaviad nnsinrktms
       61 lfgapcklqs vyphpadskr plfflvdpvh likcvrnnwi nqrnagtcmf fpsptgpnik
      121 ppiltasfkt lrelhskeqg qliksaptls skslnpsnie rqnvklalri fspstaaalr
      181 acgprleldy vsgtaqflet itkwwnivnv ktcnkgtrvr eelqspitsa sspqiqflls
      241 ivewldlwqs lkfdtgiltr ethsalrltt dtlvkvtryc ldemnfeyvl lgkfqtdcle
      301 erfgkyrqls gaqyhisirq vyeserklrl qnilelpefe aatdavavnd avlevfgiev
      361 tgddyakapn lpaatyvagy cahaafkkls cmtckenfml edeievegge lvkamtrgsl
      421 kfpqpaivna vltaeivldk lrseqhatqf halpnqkeal lalthdvlnd gidfdvceng
      481 htpqlvrhyv lsaaantlln nlckrkk
//
LOCUS       XP_077492102             362 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103038 [Amblyomma americanum].
ACCESSION   XP_077492102
VERSION     XP_077492102.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635976.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..362
                     /product="uncharacterized protein LOC144103038"
                     /calculated_mol_wt=37487
     Region          141..197
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..362
                     /gene="LOC144103038"
                     /coded_by="XM_077635976.1:430..1518"
                     /db_xref="GeneID:144103038"
ORIGIN      
        1 mtvdlspgqq qskcsrqqhd gdampggpap psflirdils epgaqgaaaa aaaaaaaaaa
       61 asvaaqclvp mpfaqhhplh pmahhhlprp pspadsysrh sdrddghgse spdrtlespp
      121 gsssggggag gmcalkmkkq rkartaftdh qlqtleksfe rqkylsvqdr melaaklnlt
      181 dtqvktwyqn rrtkwkrqta vglellaeag nyaavqrmlq tapywlssyt glspqsslst
      241 maaldlyyrq aalqkpmayr lypgvpppgv pgvappgtga pphghpppga asnglpvvsp
      301 qmppcssaag aspsatarps psspaapaas havlalptts ssslssssss assfrptsre
      361 sc
//
LOCUS       XP_077492103             419 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103054 [Amblyomma americanum].
ACCESSION   XP_077492103
VERSION     XP_077492103.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635977.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..419
                     /product="uncharacterized protein LOC144103054"
                     /calculated_mol_wt=44405
     Region          <201..>235
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     CDS             1..419
                     /gene="LOC144103054"
                     /coded_by="XM_077635977.1:1..1260"
                     /db_xref="GeneID:144103054"
ORIGIN      
        1 mssggaaiaa lagrgnrlaa dadkeyeivl ptlptgrvvl stvflhgdvr arpyrvedfr
       61 dalanagalp dvlalgayqi nhvwavtlss aeatkklaal kelqvkghrc iifdpedqqv
      121 klrlhwmlhg vadddirtaf aafgnvtevt rerwrvqgmk ekgsttrtvl lklkpgmkvd
      181 dlphqlrvag elalvvvpgr pmqclrchgt ghvrrdckvp rcsqcrrygh adadcvrtya
      241 satglgkand taelmdvaea eeaatgtdea gksastlatc vssgdsskaa depasqppta
      301 dslgadnvta gngasptepe avqsndpnkd dhgksgartt svtppvttra krphdqtspe
      361 gdkvvapgve eppaktsqsr rpkfrprpnl sddkragdkv lleqadvlpp ddtggnsgv
//
LOCUS       XP_077492104             185 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103070 [Amblyomma americanum].
ACCESSION   XP_077492104
VERSION     XP_077492104.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635978.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..185
                     /product="uncharacterized protein LOC144103070"
                     /calculated_mol_wt=21348
     CDS             1..185
                     /gene="LOC144103070"
                     /coded_by="XM_077635978.1:1..558"
                     /db_xref="GeneID:144103070"
ORIGIN      
        1 mpksilqdpl llsrlkwsei edllclevfg etrryplsvr glinidamds gvfrtyfrfe
       61 kddlrklqsa lripemvvtp qkvsvpgdea lcitlrrlay pnrlcdledl fgrhssalss
      121 ltnevlrhvd ecffhllddf nnhkwlnlnt lekfsqvsrk ykifrvrisc vdisntfahs
      181 sghsc
//
LOCUS       XP_077492106             134 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103101 [Amblyomma americanum].
ACCESSION   XP_077492106
VERSION     XP_077492106.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635980.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..134
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..134
                     /product="uncharacterized protein LOC144103101"
                     /calculated_mol_wt=14582
     CDS             1..134
                     /gene="LOC144103101"
                     /coded_by="XM_077635980.1:61..465"
                     /db_xref="GeneID:144103101"
ORIGIN      
        1 mknrnkkygs rdrqalhlgr lvshgsstvs ssflgclpin ddgrveesle vgiicqkaht
       61 adgydlsfyc qlsmrhqtar kcschgrtac lkeaeettsh nlrqappfav pvlqylycka
      121 qataaatqae pvla
//
LOCUS       XP_077492107             436 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103117 [Amblyomma americanum].
ACCESSION   XP_077492107
VERSION     XP_077492107.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635981.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..436
                     /product="uncharacterized protein LOC144103117"
                     /calculated_mol_wt=48810
     Region          11..50
                     /region_name="F-box_SF"
                     /note="F-box domain superfamily; cl45894"
                     /db_xref="CDD:459239"
     Site            order(11,14..15,18..19,23,25..26,30..32,34)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438852"
     CDS             1..436
                     /gene="LOC144103117"
                     /coded_by="XM_077635981.1:132..1442"
                     /db_xref="GeneID:144103117"
ORIGIN      
        1 mdsvacadyl vvsqildyvp vsdlfntsev crlwqvislg llrrklrylc ychikseekc
       61 qttasglpkf ledfcrdlrr fiqiarqagc sptfgflsys snpkdekavv qqvqrqlppe
      121 cvvvyhrskt itmrprddpt kacsvhrgit gaflfgpspg apsacrsrsn srsrsnshss
      181 nssssrssns shnsraaeap gssrscrate apdvpappyk rqcsfkrsrl eklsklwrra
      241 vlrrpvedpc lrnvapgvml fhsfripmed grcltthwre ehtgrvvavs fmrlfqqrda
      301 trvrsllssf rrefspcrsn snsssnssnn nihsnhrass stprspagns tssrdssprd
      361 tfvivlqnsm edtrelqalv elfpqvpvms isgeflrdee llllgeplaq rkvivmvvrl
      421 lpsgaenhvi vddkid
//
LOCUS       XP_077492108             340 aa            linear   INV 25-NOV-2025
DEFINITION  sterile alpha motif domain-containing protein 3-like [Amblyomma
            americanum].
ACCESSION   XP_077492108
VERSION     XP_077492108.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635982.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..340
                     /product="sterile alpha motif domain-containing protein
                     3-like"
                     /calculated_mol_wt=38438
     CDS             1..340
                     /gene="LOC144103148"
                     /coded_by="XM_077635982.1:671..1693"
                     /db_xref="GeneID:144103148"
ORIGIN      
        1 mkkstlfptr hfytrvtsll lenhpqlrdv vgsgadswkv alryrfknqr rvlpsdgrig
       61 enrtkfaarr kcseaagame lerhkvrkla aatsaiaged easfvmhtqw lmaecrkasp
      121 decqmlerms vtsrrriadi sastvneake rypylmnfer fcsdfqtmtk inpsaciseg
      181 inkimklalm klitceastl dslrsaaema perlrtrhiq tvavlrilsa nvkelnglsa
      241 lfvqeedlsv patpcivftg qeiedadfly levdreklfr vknaeeglaa vlsaywlfnv
      301 qyerklfntl vvlerlflgl alttprvvat kflnkiaksv
//
LOCUS       XP_077492109             357 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103175 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077492109
VERSION     XP_077492109.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635983.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..357
                     /product="uncharacterized protein LOC144103175 isoform X1"
                     /calculated_mol_wt=39585
     Region          22..51
                     /region_name="F-box_SF"
                     /note="F-box domain superfamily; cl45894"
                     /db_xref="CDD:459239"
     Site            order(23..25,28..29,32,43..45,47,50)
                     /site_type="other"
                     /note="F-box motif"
                     /db_xref="CDD:438852"
     Site            order(24,27..28,31..32,36,38..39,43..45,47)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438852"
     CDS             1..357
                     /gene="LOC144103175"
                     /coded_by="XM_077635983.1:89..1162"
                     /db_xref="GeneID:144103175"
ORIGIN      
        1 mpgearsptt slagdkrmgc sefvaqkvla ymsvrdlfnc clvnrmwqrl slpllrkkld
       61 wvlccaprkd gdescclqny cshlrrlcyv arqvkkkpal hfllisasnd dersvvngvr
      121 qslphdcvtv lfkskldrtl llegsckgln gvvvfkdvak gnrwalkrrn kgdahfessa
      181 sdadslgrvs kflrrwvlgd rgsspvacfk ntsagvltfq srlsvpldda ghittyciek
      241 atgyklqvtt atlhakmkrr svqlilslrd tfknlgstiv vvfqkdsvdm lvmdylrcll
      301 ndvpvismgg qtmtearayt wdstatpprg grvtvmvltl lpkasekifk ikslapa
//
LOCUS       XP_077492110             277 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103175 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077492110
VERSION     XP_077492110.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635984.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..277
                     /product="uncharacterized protein LOC144103175 isoform X2"
                     /calculated_mol_wt=30855
     Region          22..51
                     /region_name="F-box_SF"
                     /note="F-box domain superfamily; cl45894"
                     /db_xref="CDD:459239"
     Site            order(23..25,28..29,32,43..45,47,50)
                     /site_type="other"
                     /note="F-box motif"
                     /db_xref="CDD:438852"
     Site            order(24,27..28,31..32,36,38..39,43..45,47)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438852"
     CDS             1..277
                     /gene="LOC144103175"
                     /coded_by="XM_077635984.1:89..922"
                     /db_xref="GeneID:144103175"
ORIGIN      
        1 mpgearsptt slagdkrmgc sefvaqkvla ymsvrdlfnc clvnrmwqrl slpllrkkld
       61 wvlccaprkd gdescclqny cshlrrlcyv arqvkkkpal hfllisasnd dersvvngvr
      121 qslphdcvtv lfkskldrtl llegsckgln gvvvfkdvak gnrwalkvtt atlhakmkrr
      181 svqlilslrd tfknlgstiv vvfqkdsvdm lvmdylrcll ndvpvismgg qtmtearayt
      241 wdstatpprg grvtvmvltl lpkasekifk ikslapa
//
LOCUS       XP_077492111             146 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103202 [Amblyomma americanum].
ACCESSION   XP_077492111
VERSION     XP_077492111.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077635985.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..146
                     /product="uncharacterized protein LOC144103202"
                     /calculated_mol_wt=15820
     CDS             1..146
                     /gene="LOC144103202"
                     /coded_by="XM_077635985.1:281..721"
                     /db_xref="GeneID:144103202"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrppfa altsvlccqr pasigpcvlp afgcvippdp
       61 fmlktkscme vvqatklrvi fardleqagk lvdgqwysls kasffsktkt iflgswamyl
      121 gtcgqdnagq hasasskkkh etvnky
//
LOCUS       XP_077492160             404 aa            linear   INV 25-NOV-2025
DEFINITION  dnaJ homolog subfamily B member 6-B-like [Amblyomma americanum].
ACCESSION   XP_077492160
VERSION     XP_077492160.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636034.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..404
                     /product="dnaJ homolog subfamily B member 6-B-like"
                     /calculated_mol_wt=42951
     Region          4..>114
                     /region_name="PRK14290"
                     /note="chaperone protein DnaJ; Provisional"
                     /db_xref="CDD:172778"
     CDS             1..404
                     /gene="LOC144103267"
                     /coded_by="XM_077636034.1:161..1375"
                     /db_xref="GeneID:144103267"
ORIGIN      
        1 msgadeyyra levprnatqd dirrayrrla lrwhpdknpd nkddaekrfk aiaeayevls
       61 detkrrqydl ygpsgydtsq qtdvgasscs tssrhggvfs afrdpeelfr effgtsdpfe
      121 elfrtvrqgs asmhphakss npsrpsgggg svqtggspsf hhcgldpgri lrpgfffdld
      181 dllfgacgsv ptgpgglgfp sqggfaglpt eqmssvrysn gkrcetrtiv qdgvktvlcf
      241 edgrmvsrtv ngvsqdvpkv sddgrddsqq gaysssashk asesgssphs aegaarqaks
      301 gksvgsdlsp kvggkasshs nkqpptrqss kahksrshta sksaikvprk egseatatga
      361 panapqkppn qtptptptpv pppqqqqpna qaagvkrkps ktgk
//
LOCUS       XP_077492168             475 aa            linear   INV 25-NOV-2025
DEFINITION  cellular communication network factor protein Ccn [Amblyomma
            americanum].
ACCESSION   XP_077492168
VERSION     XP_077492168.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636042.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..475
                     /product="cellular communication network factor protein
                     Ccn"
                     /calculated_mol_wt=50612
     Region          <185..>213
                     /region_name="IGFBP"
                     /note="Insulin-like growth factor binding protein;
                     pfam00219"
                     /db_xref="CDD:459717"
     Region          326..371
                     /region_name="TSP1_CCN"
                     /note="CCN3 Nov like TSP1 domain; pfam19035"
                     /db_xref="CDD:465952"
     CDS             1..475
                     /gene="Ccn"
                     /coded_by="XM_077636042.1:1109..2536"
                     /db_xref="GeneID:144103277"
ORIGIN      
        1 mdqkraavtw yrssrgarap prirmtfllv aclascthha raqiddnsld ysvyagptrg
       61 ssrcpdstgc iagndsgrdg tsaesartsy nnaaevstld geegpktvtv etdtttatat
      121 sqpeswwptl vasrppavpv eytiragktn itvngtrtgh rgavqcgpsc rcssggpheg
      181 geggarcpdg vslvrdgcnc csicarqqgd tcdavqlcda rrqlhcvypd rnasvgtckv
      241 vqgqscqveg lgqykdgatf kldcrtqcsc qngtygcvsl cphegvrpsa ecrnarlvrl
      301 rgnccrewlc dhdtlkqpla larascerha gdwspcsasc gvgvstrlvt dfrlgqcrsr
      361 netricnvrp ctaldaagaa gsagssgsrh rtrrnhlcra tvrascaapl tdganctsva
      421 pqtpkycgsc pgrrccfpvl tttttlpfhc fedggwvtrh kdvmtivrcr ckpkc
//
LOCUS       XP_077492192              88 aa            linear   INV 25-NOV-2025
DEFINITION  ixochymostatin-like [Amblyomma americanum].
ACCESSION   XP_077492192
VERSION     XP_077492192.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636066.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..88
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..88
                     /product="ixochymostatin-like"
                     /calculated_mol_wt=9554
     Region          29..85
                     /region_name="TIL"
                     /note="trypsin inhibitor-like cysteine rich domain;
                     cd19941"
                     /db_xref="CDD:410995"
     Site            order(38,43,57,61)
                     /site_type="other"
                     /note="reactive site [polypeptide binding]"
                     /db_xref="CDD:410995"
     CDS             1..88
                     /gene="LOC144103310"
                     /coded_by="XM_077636066.1:73..339"
                     /db_xref="GeneID:144103310"
ORIGIN      
        1 mfqsvnihgq gereesdsvl rwpptpqecg egetwkecvs gscaeaacer pvvgpactdd
       61 cnygcycadg fyrnqhkacv trdkcsqa
//
LOCUS       XP_077492201             145 aa            linear   INV 25-NOV-2025
DEFINITION  nucleoredoxin-like protein 2 [Amblyomma americanum].
ACCESSION   XP_077492201
VERSION     XP_077492201.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636075.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..145
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..145
                     /product="nucleoredoxin-like protein 2"
                     /calculated_mol_wt=16204
     Region          9..142
                     /region_name="Thioredoxin_like"
                     /note="Protein Disulfide Oxidoreductases and Other
                     Proteins with a Thioredoxin fold; cl00388"
                     /db_xref="CDD:469754"
     CDS             1..145
                     /gene="LOC144103321"
                     /coded_by="XM_077636075.1:145..582"
                     /db_xref="GeneID:144103321"
ORIGIN      
        1 masifsgkml ikkdgklypa dealrdkqli clyfaahwcp pcrmftpvla nvykvakgdn
       61 laievifisa drnkndmlny mktshgdwyg lpfddplqge veerfkvvgv ptllvmkadg
      121 tmvcangkkv vqergvqafr dwlgn
//
LOCUS       XP_077492210             135 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103338 [Amblyomma americanum].
ACCESSION   XP_077492210
VERSION     XP_077492210.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636084.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..135
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..135
                     /product="uncharacterized protein LOC144103338"
                     /calculated_mol_wt=14149
     CDS             1..135
                     /gene="LOC144103338"
                     /coded_by="XM_077636084.1:398..805"
                     /db_xref="GeneID:144103338"
ORIGIN      
        1 mettcsceed alldgfvecp gisslvhnva ttytsraays trdvslqtpa sgssacskhv
       61 srfgmsasns sasaqlvllr vitttgggnp eqnnmeadge haelrhcdtg qndsersdav
      121 tflpghgrhp nvlqh
//
LOCUS       XP_077492215             135 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103338 [Amblyomma americanum].
ACCESSION   XP_077492215
VERSION     XP_077492215.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636089.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..135
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..135
                     /product="uncharacterized protein LOC144103338"
                     /calculated_mol_wt=14149
     CDS             1..135
                     /gene="LOC144103338"
                     /coded_by="XM_077636089.1:397..804"
                     /db_xref="GeneID:144103338"
ORIGIN      
        1 mettcsceed alldgfvecp gisslvhnva ttytsraays trdvslqtpa sgssacskhv
       61 srfgmsasns sasaqlvllr vitttgggnp eqnnmeadge haelrhcdtg qndsersdav
      121 tflpghgrhp nvlqh
//
LOCUS       XP_077492225             169 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103356 [Amblyomma americanum].
ACCESSION   XP_077492225
VERSION     XP_077492225.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636099.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..169
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..169
                     /product="uncharacterized protein LOC144103356"
                     /calculated_mol_wt=18843
     Region          21..41
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(21,24,37,41)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          52..72
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(52,55,68,72)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(57,59,61,63..64,67..68,71,85,87,91..92,95..96,99,
                     113,115,117,119..120,123..124)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          <74..129
                     /region_name="SFP1"
                     /note="Putative transcriptional repressor regulating G2/M
                     transition [Transcription / Cell division and chromosome
                     partitioning]; COG5189"
                     /db_xref="CDD:227516"
     Region          80..100
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          92..117
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          108..126
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..169
                     /gene="LOC144103356"
                     /coded_by="XM_077636099.1:13..522"
                     /db_xref="GeneID:144103356"
ORIGIN      
        1 mealptvdgk rsaapprifs cdtcgalfpa qdllemhrst mhpprpqgrh rcaycdfsrd
       61 skshdtrhgr thtgdrpfvc evcdkgftrr wnlsrhmlmh lgqkphqcpe cgqrfsrgyl
      121 larhrrqqhs pmaglcstca laarkssern snmnatgahc tgctvstlp
//
LOCUS       XP_077492231             214 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103363 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077492231
VERSION     XP_077492231.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636105.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..214
                     /product="uncharacterized protein LOC144103363 isoform X1"
                     /calculated_mol_wt=24883
     CDS             1..214
                     /gene="LOC144103363"
                     /coded_by="XM_077636105.1:52..696"
                     /db_xref="GeneID:144103363"
ORIGIN      
        1 maylrfrwsq elqsssglmd rpraaapglv prfgqggaih rpllrrphpr pvedvldelw
       61 plindfcgci neyldkqhpy rfqrrpgytp ewstvwevyk hvslaqeymg nllkrsedep
      121 gtlidlaeaf vneleklskv lrhkaglpea ewnltapnvl dhivnprere illrrhqkiv
      181 tlhdslvyca mellpqphrr qtpkadphrr qtpk
//
LOCUS       XP_077492235             196 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103363 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077492235
VERSION     XP_077492235.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636109.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..196
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..196
                     /product="uncharacterized protein LOC144103363 isoform X2"
                     /calculated_mol_wt=22741
     CDS             1..196
                     /gene="LOC144103363"
                     /coded_by="XM_077636109.1:144..734"
                     /db_xref="GeneID:144103363"
ORIGIN      
        1 mdrpraaapg lvprfgqgga ihrpllrrph prpvedvlde lwplindfcg cineyldkqh
       61 pyrfqrrpgy tpewstvwev ykhvslaqey mgnllkrsed epgtlidlae afvnelekls
      121 kvlrhkaglp eaewnltapn vldhivnpre reillrrhqk ivtlhdslvy camellpqph
      181 rrqtpkadph rrqtpk
//
LOCUS       XP_077492242             142 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103381 [Amblyomma americanum].
ACCESSION   XP_077492242
VERSION     XP_077492242.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636116.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..142
                     /product="uncharacterized protein LOC144103381"
                     /calculated_mol_wt=15385
     CDS             1..142
                     /gene="LOC144103381"
                     /coded_by="XM_077636116.1:267..695"
                     /db_xref="GeneID:144103381"
ORIGIN      
        1 mnrmtavvpa ilrarnssqa rdpthrppfa altsvlccqw qasigpcvlp afgcvippdp
       61 fmlktkscme vvqatklrvi fardleqagk lvdgqwysls kasffsktkt iflgswamyl
      121 gtcgqdntgq hasasskkst kq
//
LOCUS       XP_077492266             135 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103415 [Amblyomma americanum].
ACCESSION   XP_077492266
VERSION     XP_077492266.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636140.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..135
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..135
                     /product="uncharacterized protein LOC144103415"
                     /calculated_mol_wt=14149
     CDS             1..135
                     /gene="LOC144103415"
                     /coded_by="XM_077636140.1:400..807"
                     /db_xref="GeneID:144103415"
ORIGIN      
        1 mettcsceed alldgfvecp gisslvhnva ttytsraays trdvslqtpa sgssacskhv
       61 srfgmsasns sasaqlvllr vitttgggnp eqnnmeadge haelrhcdtg qndsersdav
      121 tflpghgrhp nvlqh
//
LOCUS       XP_077492311             137 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103475 [Amblyomma americanum].
ACCESSION   XP_077492311
VERSION     XP_077492311.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636185.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..137
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..137
                     /product="uncharacterized protein LOC144103475"
                     /calculated_mol_wt=13517
     CDS             1..137
                     /gene="LOC144103475"
                     /coded_by="XM_077636185.1:73..486"
                     /db_xref="GeneID:144103475"
ORIGIN      
        1 masklaitll vllalavcnt sgqgqgkglg rgrgqghgqg rgqgqghgqg rgkhgggpsv
       61 ggplvadggp vpgggggqfr esggfrqtqg hrveegfeqt dfdnqgrgng aaflgtnsgv
      121 lqsgllqevk gkfkimg
//
LOCUS       XP_077492322             123 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103493 [Amblyomma americanum].
ACCESSION   XP_077492322
VERSION     XP_077492322.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636196.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..123
                     /product="uncharacterized protein LOC144103493"
                     /calculated_mol_wt=13579
     CDS             1..123
                     /gene="LOC144103493"
                     /coded_by="XM_077636196.1:173..544"
                     /db_xref="GeneID:144103493"
ORIGIN      
        1 mlrkcssrpr dsrvamllsv scrsmmaagl lapcvacyrt llrvfcwihe adpsavvral
       61 ldessvmarf glaltkrsyi myrppnelvt savlytaikp ssleltteis aknlirkkrg
      121 slk
//
LOCUS       XP_077492329             657 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103501 [Amblyomma americanum].
ACCESSION   XP_077492329
VERSION     XP_077492329.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636203.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..657
                     /product="uncharacterized protein LOC144103501"
                     /calculated_mol_wt=71584
     Region          <483..629
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..657
                     /gene="LOC144103501"
                     /coded_by="XM_077636203.1:302..2275"
                     /db_xref="GeneID:144103501"
ORIGIN      
        1 mdnaerlgda nstpaalpeh kddaalaeqp psleaagaid sgdcnadcsp siqravtspl
       61 vdlfkddssg rhlaingnnk pseaqsdrsn pctveptlle tcmvpangdv kngprkiims
      121 aelhvcgags akeesqpkga clfsrpeapg griafhades pdtvmrnqfs cdsdlgssnp
      181 fkgdvlnitp sgsdkleavn ddaakspvtg navakvpplr svrpaagdkl tkagapagss
      241 rncsqltmde macvsdqlll vdtctqapve knlpaeqnkg nevkqkpstv cpklslcglq
      301 peelrrktyq llrrqrdeay dhvgrlekei allqkfydrh cefllnklrk legenrrlsa
      361 anavqrrlmq ellprlgdss lpgtvsdqlk lqgeggakpp lhsvtpaggd elikagspag
      421 sshnsrqlav gemaclsdkl lpvdsgtpas vqenlhaehn ngknvkqkpg vvcpnlslca
      481 qpaeelrrqr aqaydrverl ekeivllrnf ydrhseflln kvrklneknr tlsatnaeqr
      541 llmqellsrr gnsslpdair eqeelqaevk rlrkqillre eelqdlkanf lrmrkasten
      601 yraathdink vdtmlqrvhe afeaafddvc rspalkqlll nlrsggggwk dttpssk
//
LOCUS       XP_077492346             110 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103519 [Amblyomma americanum].
ACCESSION   XP_077492346
VERSION     XP_077492346.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636220.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..110
                     /product="uncharacterized protein LOC144103519"
                     /calculated_mol_wt=12032
     Region          37..>86
                     /region_name="BEN"
                     /note="The BEN domain is found in diverse animal proteins;
                     cl11158"
                     /db_xref="CDD:472128"
     CDS             1..110
                     /gene="LOC144103519"
                     /coded_by="XM_077636220.1:193..525"
                     /db_xref="GeneID:144103519"
ORIGIN      
        1 malsytfhfq idigggvlie etaleklrss cpgapakfar glmrqlftad elrgkslfgr
       61 ksnshpdasl realdpvrvn aiigftiref kadpirlkss lssllsreik
//
LOCUS       XP_077492397              98 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103594 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077492397
VERSION     XP_077492397.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636271.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..98
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..98
                     /product="uncharacterized protein LOC144103594 isoform X1"
                     /calculated_mol_wt=10007
     CDS             1..98
                     /gene="LOC144103594"
                     /coded_by="XM_077636271.1:134..430"
                     /db_xref="GeneID:144103594"
ORIGIN      
        1 mgkeiirivv ilavavsfaa aqwqvrpivs vsgksardfg anvgarvqgv vhkfpnsgak
       61 iiasaeasks fghagghgwn gppqgqvgvr veipigrk
//
LOCUS       XP_077492400              97 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103594 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077492400
VERSION     XP_077492400.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636274.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..97
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..97
                     /product="uncharacterized protein LOC144103594 isoform X2"
                     /calculated_mol_wt=9907
     CDS             1..97
                     /gene="LOC144103594"
                     /coded_by="XM_077636274.1:134..427"
                     /db_xref="GeneID:144103594"
ORIGIN      
        1 mgkeiirivv ilaavsfaaa qwqvrpivsv sgksardfga nvgarvqgvv hkfpnsgaki
       61 iasaeasksf ghagghgwng ppqgqvgvrv eipigrk
//
LOCUS       XP_077492429             229 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103635 [Amblyomma americanum].
ACCESSION   XP_077492429
VERSION     XP_077492429.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636303.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..229
                     /product="uncharacterized protein LOC144103635"
                     /calculated_mol_wt=24545
     CDS             1..229
                     /gene="LOC144103635"
                     /coded_by="XM_077636303.1:24..713"
                     /db_xref="GeneID:144103635"
ORIGIN      
        1 msghtlrfsh ihpdwpsary srgtavapdc nvrglacvdr kwgapaatap mlkslrivtl
       61 egrrsscspi asspwsgdmt antenkaltt pastalqvss hhvcmrgtct plgkalrris
      121 syhqgdltpk asrpsaqvrl svctaphgsp paawwfrskh hwlrrrqrap pswrctgied
      181 agtqhaqlrq giaavlmlll ltcgsevdsl vaqsvpgmpw tgalgdgdt
//
LOCUS       XP_077492443             117 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103654 [Amblyomma americanum].
ACCESSION   XP_077492443
VERSION     XP_077492443.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636317.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..117
                     /product="uncharacterized protein LOC144103654"
                     /calculated_mol_wt=13213
     CDS             1..117
                     /gene="LOC144103654"
                     /coded_by="XM_077636317.1:20..373"
                     /db_xref="GeneID:144103654"
ORIGIN      
        1 msqellphig vpvcfkeris iaslqrlklc smefpsldkd nehvtawtlp vgrylrtela
       61 pftrclplsl htprvtcvrt qrrsarpvas pelvvgvlrl whrltwpsfd eseaplg
//
LOCUS       XP_077492460             148 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103671 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077492460
VERSION     XP_077492460.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636334.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="uncharacterized protein LOC144103671 isoform X1"
                     /calculated_mol_wt=16759
     CDS             1..148
                     /gene="LOC144103671"
                     /coded_by="XM_077636334.1:168..614"
                     /db_xref="GeneID:144103671"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedrrr rhlnrrltat mkrlsgvhvl
       61 nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar pgevyvvhrc
      121 rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077492467              98 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103671 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077492467
VERSION     XP_077492467.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636341.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..98
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..98
                     /product="uncharacterized protein LOC144103671 isoform X2"
                     /calculated_mol_wt=10604
     CDS             1..98
                     /gene="LOC144103671"
                     /coded_by="XM_077636341.1:218..514"
                     /db_xref="GeneID:144103671"
ORIGIN      
        1 mkrlsgvhvl nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar
       61 pgevyvvhrc rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077492483             747 aa            linear   INV 25-NOV-2025
DEFINITION  O-acyltransferase like protein-like [Amblyomma americanum].
ACCESSION   XP_077492483
VERSION     XP_077492483.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636357.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..747
                     /product="O-acyltransferase like protein-like"
                     /calculated_mol_wt=83551
     Region          56..201
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          310..696
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..747
                     /gene="LOC144103697"
                     /coded_by="XM_077636357.1:174..2417"
                     /db_xref="GeneID:144103697"
ORIGIN      
        1 maqsvrfiwl vtmvaaasam kfdftaldpk iildkirdtm emlmsmlwvt gaspqcaktl
       61 kqlirdtfgg kvwalyvvda takpepgile gafssvgsqt eclkahafln vsgkdsrqrp
      121 whvgfvqgsf ckiryriglr eilsavdegr aspnmldlvd gilgddlyll geienvvedf
      181 pgffgmcvps acsandiqsv fnfitpivks ftnitisvqv tacktreeve aidwtpsffc
      241 avvvlgvilf fviigsllsl ldnlkptkns vhekrrswvg dlfrcfsaps ntrrllsmdd
      301 ergemacvvg lralsvvwfi mghflllhsm mltknlvllk dkikdisiqf ivnftpagdt
      361 vlcllglmmv ylsnrrlree navqtkryll kyllhrvtrl aplmvlttvl vapvfsqlgq
      421 gptwefeser mvaqcrssww fnvlhinnfl prkqqcleht wlfavmmqft vagvllapvf
      481 yhrpavgrvl sisgivasms ltlvftllyd lgpxxxfrey kpgtrntyte mvyirpytrv
      541 gvfltgmlag eflshrrkin ihkvpvflgw lcclatllal ihapfkwnrg lelpgteasa
      601 aygafsrvlw aaaicwvvva cahgyggwln nvlslrgwqp lsrllyslym asplviaysn
      661 gvrehayfls ydamsyvlih hfvlalvaat vlslfleqpf vrlealvaqr laarrapspe
      721 amvqvprier hwldkghenp afakekl
//
LOCUS       XP_077492495             379 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103708 [Amblyomma americanum].
ACCESSION   XP_077492495
VERSION     XP_077492495.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636369.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..379
                     /product="uncharacterized protein LOC144103708"
                     /calculated_mol_wt=40939
     CDS             1..379
                     /gene="LOC144103708"
                     /coded_by="XM_077636369.1:73..1212"
                     /db_xref="GeneID:144103708"
ORIGIN      
        1 mccpeslpsl qelypwcgsl ylgterttav kqemklcgrs lpsrkhglsv gelpdsclwt
       61 magkligctr iysessfaer krsgecalkp rtsestatpl kawssescpa wrhrspacsg
      121 pncatrwsrs skpstrwaar rtssrgqgss akcrsrfskt rmsltswimc spstpyhcss
      181 trpagcwhrs nttrttastp assrpawggt rrprgpgcgt wspgirrevt gaarhtcaar
      241 qrdavrpsha hlggatcddl svrrcylgiq cgrrspkmdp ecacavvleh vaahavragg
      301 gglneeprpr assqrapggk yehavprrpg staerksegq lwwsssadaa neesaadhra
      361 awssmgkygp sldqlgtgr
//
LOCUS       XP_077492505             222 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103719 [Amblyomma americanum].
ACCESSION   XP_077492505
VERSION     XP_077492505.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636379.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..222
                     /product="uncharacterized protein LOC144103719"
                     /calculated_mol_wt=24552
     Region          57..200
                     /region_name="Grp7_allergen"
                     /note="Group 7 allergen; pfam16984"
                     /db_xref="CDD:319052"
     CDS             1..222
                     /gene="LOC144103719"
                     /coded_by="XM_077636379.1:363..1031"
                     /db_xref="GeneID:144103719"
ORIGIN      
        1 mdawkifvfl lgtatleclg slrkhnprtt kpgtlpvphs ndtagaftkl iqfimanqsl
       61 dplpiprqkf vvdvlghiem nnalltglss lqrtglvyly vndsgltlhv dvgsgeltmn
      121 fttklkimfv krevnvsitt sstqvildvg etvdrklrle glniakleni rvrlsmdgig
      181 gaifnsflkg fttvfkrvir staqrevrka lekeikkine vl
//
LOCUS       XP_077492514            1393 aa            linear   INV 25-NOV-2025
DEFINITION  intraflagellar transport protein rempA isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077492514
VERSION     XP_077492514.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636388.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1393
                     /product="intraflagellar transport protein rempA isoform
                     X1"
                     /calculated_mol_wt=153609
     Region          17..54
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          60..112
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          108..287
                     /region_name="YvrE"
                     /note="Sugar lactone lactonase YvrE [Carbohydrate
                     transport and metabolism]; COG3386"
                     /db_xref="CDD:442613"
     Region          117..161
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          169..208
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          223..260
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          268..310
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          315..357
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          367..405
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          409..444
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          458..500
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          746..1001
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     CDS             1..1393
                     /gene="rempA"
                     /coded_by="XM_077636388.1:539..4720"
                     /db_xref="GeneID:144103730"
ORIGIN      
        1 marpcpanch askraqvsal swhpwaplla lgwdsgevcv wddrqamlhe pqtqhkravs
       61 rllwngtrlv sidavgtcig wkagrafata fyhelrdsla dlafrdrril aspsaapafd
      121 ggeallpsds kggilaltdd daapndfyvg sttgviyfvs engscgdvyq mdagvarllh
      181 dahrdtlvvv tdtltlaqfa igddgslseq srfkvsghga dmavtwagvq ggviaiaage
      241 ssvrllsfqt gettvlslpd adygetvsci aadesgktll vgtnrgrvvv waltensvga
      301 thwrsqlaas lrspvrelaw sethriagtl tnddmfllve qeacfnfreg ylalqtaakq
      361 vfidsvssgn qivlqteipv rslfisrqhv vvsngkrlff yetlpgsksa kflgamntas
      421 sliglhetsv ylvengklna ktfqgilkqa ipvsedevsa vcldisgifl alglsngrvr
      481 vwdlsrreak lhaelntpvv akerevaqri ssikcncvgn kvsilvaeds srhrgirres
      541 salyvwsied dslfeydfdt gesrtgkavs dlrakgtvrg raplshywde tdprllvcea
      601 rrivdgyaka dasgnqepsv gvvslfvtte hgileqdvfp lddrfrlmgv qvpffyllak
      661 htgevsesge aekvpgsklv frlpmrnfvg lqdcddsvrd aimtfsffmt lrdmdeafka
      721 ikaikneavw anmarmcvkt krldvakicl gkmahargaw slrravetep eleaqtaila
      781 lqlgmvqeaq ellescgryd llvrlledsg qweraidvar ahdrirlrat yhnyakhlea
      841 sgdvekavsm yekadthlfd vprmlfddpq aleqyvrssg spdmlkwwaq ymesvgemea
      901 alqfyeaard ylsllrvhcy fgnvdkacei anetedraac fhlarylenq dnvlqavhff
      961 ararafgnav rvckenemdd qlyslalqaa pkealeaary feqrpdglek avtlyqhggw
     1021 askaaelalq agdlgalqqa aqelgpsadp alvrttadrl aaigkhdmav lllatsgqse
     1081 aalqlcvqan vlvteeladr ltdgipaddk pklrevleki aecclkqdny hlaakkftqa
     1141 gdkvqamral lksgdtrkiv ffanvsrlre iyilaanylq tldwqrdtda aknivsfytk
     1201 arafdllasf yescaraeie drrdyekala alneaqeclq lcgedkdvsk aavrirshmd
     1261 avhkflaiqe lyadspleaa aqceamtkmd lepavrkgdl faflvqhyvs ekdyvtarrc
     1321 lqemqaavta vseyvdaevl klvgwssddr rslqkmslla rraaaasnga aneadedrys
     1381 sdgevqeels dap
//
LOCUS       XP_077492521            1223 aa            linear   INV 25-NOV-2025
DEFINITION  intraflagellar transport protein rempA isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077492521
VERSION     XP_077492521.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636395.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1223
                     /product="intraflagellar transport protein rempA isoform
                     X2"
                     /calculated_mol_wt=135234
     Region          6..318
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          53..90
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          98..140
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          145..187
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          197..235
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          239..274
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          288..330
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          576..831
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     CDS             1..1223
                     /gene="rempA"
                     /coded_by="XM_077636395.1:753..4424"
                     /db_xref="GeneID:144103730"
ORIGIN      
        1 mdagvarllh dahrdtlvvv tdtltlaqfa igddgslseq srfkvsghga dmavtwagvq
       61 ggviaiaage ssvrllsfqt gettvlslpd adygetvsci aadesgktll vgtnrgrvvv
      121 waltensvga thwrsqlaas lrspvrelaw sethriagtl tnddmfllve qeacfnfreg
      181 ylalqtaakq vfidsvssgn qivlqteipv rslfisrqhv vvsngkrlff yetlpgsksa
      241 kflgamntas sliglhetsv ylvengklna ktfqgilkqa ipvsedevsa vcldisgifl
      301 alglsngrvr vwdlsrreak lhaelntpvv akerevaqri ssikcncvgn kvsilvaeds
      361 srhrgirres salyvwsied dslfeydfdt gesrtgkavs dlrakgtvrg raplshywde
      421 tdprllvcea rrivdgyaka dasgnqepsv gvvslfvtte hgileqdvfp lddrfrlmgv
      481 qvpffyllak htgevsesge aekvpgsklv frlpmrnfvg lqdcddsvrd aimtfsffmt
      541 lrdmdeafka ikaikneavw anmarmcvkt krldvakicl gkmahargaw slrravetep
      601 eleaqtaila lqlgmvqeaq ellescgryd llvrlledsg qweraidvar ahdrirlrat
      661 yhnyakhlea sgdvekavsm yekadthlfd vprmlfddpq aleqyvrssg spdmlkwwaq
      721 ymesvgemea alqfyeaard ylsllrvhcy fgnvdkacei anetedraac fhlarylenq
      781 dnvlqavhff ararafgnav rvckenemdd qlyslalqaa pkealeaary feqrpdglek
      841 avtlyqhggw askaaelalq agdlgalqqa aqelgpsadp alvrttadrl aaigkhdmav
      901 lllatsgqse aalqlcvqan vlvteeladr ltdgipaddk pklrevleki aecclkqdny
      961 hlaakkftqa gdkvqamral lksgdtrkiv ffanvsrlre iyilaanylq tldwqrdtda
     1021 aknivsfytk arafdllasf yescaraeie drrdyekala alneaqeclq lcgedkdvsk
     1081 aavrirshmd avhkflaiqe lyadspleaa aqceamtkmd lepavrkgdl faflvqhyvs
     1141 ekdyvtarrc lqemqaavta vseyvdaevl klvgwssddr rslqkmslla rraaaasnga
     1201 aneadedrys sdgevqeels dap
//
LOCUS       XP_077492531             572 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103747 [Amblyomma americanum].
ACCESSION   XP_077492531
VERSION     XP_077492531.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636405.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..572
                     /product="uncharacterized protein LOC144103747"
                     /calculated_mol_wt=62407
     CDS             1..572
                     /gene="LOC144103747"
                     /coded_by="XM_077636405.1:40..1758"
                     /db_xref="GeneID:144103747"
ORIGIN      
        1 mdhrapeetl kqpagsqppq lppelptkta rpgilkspps lfqpqkepst ktlrfkmdqp
       61 vqsqpaalla pptptletpa sgagaqpaip sqgkleenrd vhekveisst msesiqesls
      121 esstsstsss pfqssvslds perlvppqkk raasapaagv vitpttqmvr rlggrrmalh
      181 gwfpdgitrl magrndrdks gngtphrraq esgdteqdde sspsplfedv pwrsslplqt
      241 lmgllgglal vfvislmlre srrngggnie srqleeavti gaedpdehqk smplpqitls
      301 dikeppqasv gpgkatacsg gdthcfalhd etpaestels atsatpvqpt issgqqevfg
      361 kvrqmrsdtl haprrakrrh acgpsvftyc hatrpefyye nvtgdcvatr kllqlcnrgp
      421 nrfssedscr lkcvetqrpd khcfrpvsav qcssqdhidt prwyfdgkrc rrwlsrpdrc
      481 pshdgqlftt pqecrsncta akastcsapn stsceaqrlg lyfahrtrqt tpfrcismas
      541 mttgrkgrac lmganrfptm qacrkacvht gr
//
LOCUS       XP_077492541             378 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103758 [Amblyomma americanum].
ACCESSION   XP_077492541
VERSION     XP_077492541.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636415.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..378
                     /product="uncharacterized protein LOC144103758"
                     /calculated_mol_wt=42198
     Region          35..>146
                     /region_name="SWIM"
                     /note="Uncharacterized protein, contains SWIM-type Zn
                     finger domain [Function unknown]; COG4715"
                     /db_xref="CDD:443750"
     Region          281..>378
                     /region_name="PDDEXK_nuclease-like"
                     /note="PDDEXK family nucleases; cl40440"
                     /db_xref="CDD:477358"
     CDS             1..378
                     /gene="LOC144103758"
                     /coded_by="XM_077636415.1:8..1144"
                     /db_xref="GeneID:144103758"
ORIGIN      
        1 mvqvplrtih naiplqerla kgpwtkqlkd vpdgfseqei lahqnahgaq khtvsgyrmf
       61 ksekvqnvfi ckadrstfvk atveasffla ksyntaavls sngtvvegtc ncragaagvc
      121 khvvallwfv ldlkrsgaay vrdtqsctdk prawgtgakk anvhtrefsq lrfvkhmpmk
      181 agrhltqett valrvtedhi krmhqtqknt pnamlcktle dnhflpvpvk rtlheealsq
      241 mpirlpvqsl wvehvpagyk fgsceltled swaleqatrm qsgsttwkfe rskrltashf
      301 gdvvsrqkpa dekffkklfg snqmqtkyma dglrnedaav qryigkrtvp vrsyccglcv
      361 npgvpvlgat pdrvveeg
//
LOCUS       XP_077492552             199 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103769 [Amblyomma americanum].
ACCESSION   XP_077492552
VERSION     XP_077492552.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636426.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..199
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..199
                     /product="uncharacterized protein LOC144103769"
                     /calculated_mol_wt=22373
     CDS             1..199
                     /gene="LOC144103769"
                     /coded_by="XM_077636426.1:36..635"
                     /db_xref="GeneID:144103769"
ORIGIN      
        1 mteqwpayap yqteslktff nnkrrtyrle kkkmletksg qaadeaytgk wrffrtlrfl
       61 davrmpthrs ingqcegnaa saevspeqdt neavgrtaae sglletqsap sppspgqqpg
      121 kkrrrtnera qessarwsdr qqileklasa fevhvqpedp adqfgklvaa hiksvprekv
      181 lscqaeilkv ierfssdms
//
LOCUS       XP_077492563             125 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103780 [Amblyomma americanum].
ACCESSION   XP_077492563
VERSION     XP_077492563.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636437.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..125
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..125
                     /product="uncharacterized protein LOC144103780"
                     /calculated_mol_wt=13107
     Region          42..>96
                     /region_name="BEN"
                     /note="The BEN domain is found in diverse animal proteins;
                     smart01025"
                     /db_xref="CDD:214981"
     CDS             1..125
                     /gene="LOC144103780"
                     /coded_by="XM_077636437.1:12..389"
                     /db_xref="GeneID:144103780"
ORIGIN      
        1 mmakfekspg pctpadkvdi gggvlvdksv lgllnhsnsg pgmyarvllh hlfspeelqg
       61 sslfgqqntr thskrgsldq ekvsavigft crkfpdasaa liknslasyl arlnngpaaa
      121 tpsek
//
LOCUS       XP_077492572             183 aa            linear   INV 25-NOV-2025
DEFINITION  anaphase-promoting complex subunit 10-like [Amblyomma americanum].
ACCESSION   XP_077492572
VERSION     XP_077492572.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636446.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="anaphase-promoting complex subunit 10-like"
                     /calculated_mol_wt=20263
     Region          12..181
                     /region_name="ANAPC10"
                     /note="Anaphase-promoting complex, subunit 10 (APC10);
                     pfam03256"
                     /db_xref="CDD:367420"
     Site            order(49,76,83,85,148)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176484"
     CDS             1..183
                     /gene="LOC144103791"
                     /coded_by="XM_077636446.1:193..744"
                     /db_xref="GeneID:144103791"
ORIGIN      
        1 mnassaqdhs slerqgdvre vgaeavwsls sckpgfgvnq lrdnclntfw qsdglqphev
       61 niqfhrktai qaiciyvdhk rdesytpkri avkvgstfhd lrvvntvdln eptgwvhiat
      121 qdsagrpvra fhvqiavlan hkngqdthlr qiklyspvkr asfsvlpgvn ftsaeciafs
      181 sir
//
LOCUS       XP_077492602             144 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103796 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077492602
VERSION     XP_077492602.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636476.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..144
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..144
                     /product="uncharacterized protein LOC144103796 isoform X1"
                     /calculated_mol_wt=15581
     CDS             1..144
                     /gene="LOC144103796"
                     /coded_by="XM_077636476.1:276..710"
                     /db_xref="GeneID:144103796"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdptrrppfa altsvlccqr pasigpcvlp afdcvippdp
       61 fmlktkscme vvqatklrvi fardleqagk lvdgqwysls kasffsktkt iflgswamyl
      121 gtcgqdnagq hasasskkka rnsk
//
LOCUS       XP_077492613             138 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103796 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077492613
VERSION     XP_077492613.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636487.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..138
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..138
                     /product="uncharacterized protein LOC144103796 isoform X2"
                     /calculated_mol_wt=14928
     CDS             1..138
                     /gene="LOC144103796"
                     /coded_by="XM_077636487.1:277..693"
                     /db_xref="GeneID:144103796"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdptrrppfa altsvlccqr pasigpcvlp afdcvippdp
       61 fmlktkscme vvqatklrvi fardleqagk lvdgqwyslt ktktiflgsw amylgtcgqd
      121 nagqhasass kkkarnsk
//
LOCUS       XP_077492647             603 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103817 [Amblyomma americanum].
ACCESSION   XP_077492647
VERSION     XP_077492647.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636521.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..603
                     /product="uncharacterized protein LOC144103817"
                     /calculated_mol_wt=68016
     Region          <254..314
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     Region          <302..585
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..603
                     /gene="LOC144103817"
                     /coded_by="XM_077636521.1:1..1812"
                     /db_xref="GeneID:144103817"
ORIGIN      
        1 megtvegtei tpedlrtsdw itkvgkhvke lqdltrgerr ggkdgdarle rgaesgegkk
       61 iaaeanaayk klgqknaers iasylprlpa ndykiiirpk nglalakvpt trlsaavrmv
      121 aqipwakgqd kdvlihndkq gtlifstpdm dtvwkvtkik sihidddeye vtaylaphed
      181 cgrgvvhgid pkltieelte afgnprnppi lgvrkmgkfs saivifkhet vprwvycydv
      241 plkcvlykkr yevcyrcgel ghrsdvcvsa qvkclgcgii appedhvcep kcklcgkghl
      301 tadrkckeaf rtpytikkrq weaklamevm eeearkaket evgrsrfekh qgrsrsqsgn
      361 qsewrgrses fprlpnlrea ekqkkaqpgt agpsggavvn nparpkspsr kvgwvseasq
      421 dkrdeeifqi keenrllkaq laamskqiee lrtalkpnaa pakqsqmpqn pvvraseeqg
      481 aaqppakkra kevekpiidm dtekvidmkt eqleakfeak yrqdnewrka feermlgmfq
      541 tlseqlhqrd nsvaeqihqr dnnlteqlke efakrnraye hltqqvlgnq mnqlignhgs
      601 qiq
//
LOCUS       XP_077492655             186 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103826 [Amblyomma americanum].
ACCESSION   XP_077492655
VERSION     XP_077492655.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636529.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..186
                     /product="uncharacterized protein LOC144103826"
                     /calculated_mol_wt=21812
     Region          98..118
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(98,101,114,118)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(103,105,107,109..110,113..114,117,129,131,135..136,
                     139..140,143,157,159,161,163..164,167..168)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          124..144
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          152..168
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..186
                     /gene="LOC144103826"
                     /coded_by="XM_077636529.1:80..640"
                     /db_xref="GeneID:144103826"
ORIGIN      
        1 mdretiarll tirfpslaem eakrkaanes ydsdvppeeq rhmsdqrrrl lnirpagedt
       61 ihwcwlcdrr fhlpsrliah qiyqhdfgge prpiqvpcpi cyrlcrneli mglhlgthig
      121 errcfkcgae fasatsahah krlhrnrgnf qcgfcrklfa lkskrddhcr lehfrhlkrq
      181 rsatsf
//
LOCUS       XP_077492664             186 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103836 [Amblyomma americanum].
ACCESSION   XP_077492664
VERSION     XP_077492664.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636538.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..186
                     /product="uncharacterized protein LOC144103836"
                     /calculated_mol_wt=21808
     Region          98..118
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(98,101,114,118)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          124..144
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Site            order(129,131,133,135..136,139..140,143,157,159..161,
                     163..164,168)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275370"
     Region          152..170
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     CDS             1..186
                     /gene="LOC144103836"
                     /coded_by="XM_077636538.1:346..906"
                     /db_xref="GeneID:144103836"
ORIGIN      
        1 mdretiarll tirfpslaem eakrkaanes ydsdvppeeq rhmsdqrrrl lnirpagedt
       61 ihwcwlcdrr fhlpsrliah qiyqhdfdge prpikvpcpi cyrlcrneli mglhlgthig
      121 errcfkcgae fasatsahah krlhhnrgnf qcgfcrklfa lkskrddhcr lehlrqlkrq
      181 rsatsf
//
LOCUS       XP_077492675             186 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103844 [Amblyomma americanum].
ACCESSION   XP_077492675
VERSION     XP_077492675.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636549.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..186
                     /product="uncharacterized protein LOC144103844"
                     /calculated_mol_wt=21679
     Region          98..118
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(98,101,114,118)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          124..144
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Site            order(129,131,133,135..136,139..140,143,157,159..161,
                     163..164,168)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275370"
     Region          152..170
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     CDS             1..186
                     /gene="LOC144103844"
                     /coded_by="XM_077636549.1:142..702"
                     /db_xref="GeneID:144103844"
ORIGIN      
        1 mdretiarll tirfpslaem eakrkaanes ydsdvppeeq rhmsdqrrrl lnirpagedt
       61 ihwcgicdrr fhlpsrliah qiyqhdfdge prpikvpcpi cyrlcrneli mglhlgthig
      121 errcfkcgae fasatsahah krlhhnrgnf qcgfcrklfa lkskrddhcr lehlrqlkrq
      181 rsatsf
//
LOCUS       XP_077492685             186 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103852 [Amblyomma americanum].
ACCESSION   XP_077492685
VERSION     XP_077492685.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636559.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..186
                     /product="uncharacterized protein LOC144103852"
                     /calculated_mol_wt=21941
     Region          98..118
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(98,101,114,118)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          124..144
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Site            order(129,131,133,135..136,139..140,143,157,159..161,
                     163..164,168)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275370"
     Region          152..170
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     CDS             1..186
                     /gene="LOC144103852"
                     /coded_by="XM_077636559.1:144..704"
                     /db_xref="GeneID:144103852"
ORIGIN      
        1 mdretiarll tirfpslaem eakrkaanes ydsdvppeeq rhmsdqrrrl lnirpagedt
       61 ihwcwlcdrr fhlpsrliah qiyqhdfdge prpikvpcpi cyrlcrnefi mglhlgthig
      121 errcfkcgae fasatsahah krlhhnrgnf qcrfcrklfa lkskrddhcr lehlrqlkrq
      181 rsatsf
//
LOCUS       XP_077492696             144 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103863 [Amblyomma americanum].
ACCESSION   XP_077492696
VERSION     XP_077492696.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636570.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..144
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..144
                     /product="uncharacterized protein LOC144103863"
                     /calculated_mol_wt=16999
     Region          82..102
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Site            order(87,89,91,93..94,97..98,101,115,117..119,121..122,
                     126)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275370"
     Region          110..128
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     CDS             1..144
                     /gene="LOC144103863"
                     /coded_by="XM_077636570.1:290..724"
                     /db_xref="GeneID:144103863"
ORIGIN      
        1 msdqrrrlln irpagedtih wcwlcdrrfh lpsrliahqi yqhdfggepr pikvpcpicy
       61 rlcrnefimg lhlgthiger rcfkcgaefa satsahahkr lhhnrgnfqc rfcrklfalk
      121 skrddhcrle hlrqlkrqrs atsf
//
LOCUS       XP_077492736             534 aa            linear   INV 25-NOV-2025
DEFINITION  serine/threonine-protein kinase PLK1-like [Amblyomma americanum].
ACCESSION   XP_077492736
VERSION     XP_077492736.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636610.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..534
                     /product="serine/threonine-protein kinase PLK1-like"
                     /calculated_mol_wt=59898
     Region          28..243
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Region          347..435
                     /region_name="POLO_box_1"
                     /note="First polo-box domain (PBD) of polo-like kinases
                     Plk1, Plk2, Plk3, and Plk5; cd13118"
                     /db_xref="CDD:240561"
     Site            order(353..356,425,427..431)
                     /site_type="other"
                     /note="phosphopeptide binding site [polypeptide binding]"
                     /db_xref="CDD:240561"
     Region          451..529
                     /region_name="POLO_box_2"
                     /note="Second polo-box domain (PBD) of polo-like kinases
                     Plk1, Plk2, Plk3, and Plk5; cd13117"
                     /db_xref="CDD:240560"
     Site            order(456,475,478,480)
                     /site_type="other"
                     /note="phosphopeptide binding site [polypeptide binding]"
                     /db_xref="CDD:240560"
     CDS             1..534
                     /gene="LOC144103898"
                     /coded_by="XM_077636610.1:48..1652"
                     /db_xref="GeneID:144103898"
ORIGIN      
        1 mssafpagsa atasknvlnf iidrkanrky ilgkflgkga fakcyelvdk etntvfagkv
       61 vaktllaktn hkekslmemh rrrktlteae aryflhqlll acrymvqqrv ihrdlklgnl
      121 llnesmelkv gdfglatrlg hvderrktmc gtpnyiapei lakkghsfev dvwsigcilf
      181 tllvgyppfe sstlretyar iqknvfhmps tlsstasali qkmlqaepen rpsiedilqd
      241 dfmtygplps llptsclvte prfdtlnasf svggrsvlle rnegnacpva aaasalpavs
      301 pnlaprgsqr eahlkelqsl lnhlcsirpp qrhfespdet edpasvpvfw iskwvdytdk
      361 ygigyelcde sigvlfndnt rimrlnncvs vtyvdekgle hyhtlekypp plhkkitllk
      421 hfwsymkenl latgeaaclq ecdnlvrlpc lgtwlrtrsa isfyltngtv qvnffddhtk
      481 iilcplmaav syvdsdrafh tyrlatlqrg cppemfarlk yarsildsla sass
//
LOCUS       XP_077492745            1010 aa            linear   INV 25-NOV-2025
DEFINITION  condensin-2 complex subunit G2-like [Amblyomma americanum].
ACCESSION   XP_077492745
VERSION     XP_077492745.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636619.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1010
                     /product="condensin-2 complex subunit G2-like"
                     /calculated_mol_wt=112838
     Region          189..335
                     /region_name="Condensin2nSMC"
                     /note="Condensin II non structural maintenance of
                     chromosomes subunit; pfam12422"
                     /db_xref="CDD:463573"
     CDS             1..1010
                     /gene="LOC144103903"
                     /coded_by="XM_077636619.1:40..3072"
                     /db_xref="GeneID:144103903"
ORIGIN      
        1 mesaattyes lmvaasrrga lakaellqmi telpseelgs lwtavleklq tivplllrdd
       61 ksdqeclplv alchaaahag ivcapmvnva rllsglacll kegvllnsiq alcltwivkk
      121 nrgwkrlwts tfsraielsl kehsktkted liksvflmkd glvldeseyd tvemdsserm
      181 llrdqlvpca vsanyldsak grkvvsfllt ldkslvsaiq kkvngvlplm tsahvqalaq
      241 vyvcawkava gkeqatvlee niqkimdwtv ngdissahqt nlfrflrglh aarkdralar
      301 mlyqcylpil wrglkalhyk vrlrsariff dsfpllesta apemeqelae nfrimhallv
      361 dsvpdirava vrgvckvltd ffeiipvher kalgddlink navdlnslds rvrvnqglay
      421 mtrspkahnl lralivknkd nslqdthsvq lsyvslllea rripgfrfwd vtppndllpl
      481 laesrpnlsl tlakllrnsy fdpmadernn iercfllhsl npeafrvfyr lltdvapikp
      541 imnflvaick avrlncsllr kiaaeensdp gkssgtecdq kniieqssvh ilvetlaity
      601 nslslckelc sdelkeqwky lvaimkksmn elfpltdcls mkisvlsvas llpsrevgnl
      661 amrclvmlrg fltvpeadtp mllsieakac vmflcnmrrt advlgmvtds vgklksqqtp
      721 ikshrvrfcv adqqscntel tlqvlrfmle spqlqimvlk shtvplfntw stllsivdsv
      781 qeflcasnsr mlievtmlta ydlfllltyv lhgqehpvtk ekfvaiaafe rqmiwveedl
      841 lprlqgrsgi srephleilk mylkvarsvl mttwvsfefa enflkflllc qeieedqlkt
      901 pialippvvk efmpifaktk kqsaiisalv ekvccevtad kcfspesqsg rstamdtavv
      961 sasafpstdk nsghaslkts sasrmdmava sdrgsptsvp lpctayaygl
//
LOCUS       XP_077492765             128 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103914 [Amblyomma americanum].
ACCESSION   XP_077492765
VERSION     XP_077492765.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636639.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..128
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..128
                     /product="uncharacterized protein LOC144103914"
                     /calculated_mol_wt=13198
     CDS             1..128
                     /gene="LOC144103914"
                     /coded_by="XM_077636639.1:49..435"
                     /db_xref="GeneID:144103914"
ORIGIN      
        1 mhshslgtia pvlpldvasp laappvappr advappradv appradvapp radvapprad
       61 vtpavpparp mskaprkaaq dldltvgttn etgevhvghg hyidpgtwer lltanndsaf
      121 ckqlavrl
//
LOCUS       XP_077492838             161 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103962 [Amblyomma americanum].
ACCESSION   XP_077492838
VERSION     XP_077492838.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636712.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..161
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..161
                     /product="uncharacterized protein LOC144103962"
                     /calculated_mol_wt=17499
     CDS             1..161
                     /gene="LOC144103962"
                     /coded_by="XM_077636712.1:161..646"
                     /db_xref="GeneID:144103962"
ORIGIN      
        1 mkrgkgsgms qdvqehipir rnsseqghir dgfvaarerq vprvlskspt tfnrhmkqlp
       61 gmsrlserfa khgaapants apkeagslpa wftafekkvf dnqmkifiel kslrsllaas
      121 kataalvlpq dcpklpastv advqaieeyl ssegnmealp a
//
LOCUS       XP_077492862             480 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144103983 [Amblyomma americanum].
ACCESSION   XP_077492862
VERSION     XP_077492862.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636736.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (DAPXPI010028690.1) annotated
            using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 186 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..480
                     /product="uncharacterized protein LOC144103983"
                     /calculated_mol_wt=52348
     Region          78..>212
                     /region_name="Peptidase_M14"
                     /note="Zinc carboxypeptidase; pfam00246"
                     /db_xref="CDD:459730"
     Region          340..>395
                     /region_name="Peptidase_M14NE-CP-C_like"
                     /note="Peptidase associated domain: C-terminal domain of
                     M14 N/E carboxypeptidase; putative folding, regulation, or
                     interaction domain; cd11308"
                     /db_xref="CDD:200604"
     CDS             1..480
                     /gene="LOC144103983"
                     /coded_by="XM_077636736.1:1..1443"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 186 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144103983"
ORIGIN      
        1 misstqkgaa pllaiafcft lrpldlvgaf sttsgdsvad gsanvaklrp kgpeedaveq
       61 symeqfafks ipakldvlra llaahpeatr ldtvaggeap iyalvvsaha ssraflvprv
      121 qlsaafpagd gmlvrfahyl ldrrasdphl atllkhtelh llfhlsgyrs nsfhkvcasd
      181 agsdlvrlln idasarqeas asaayrgshs ndrlvlsgvl ftdtptpark epdeqkgpkp
      241 pdhdvfshif srftqkqprq drgdscaslg skttnetqep ltkrrrqptl galekhayah
      301 rgilqsfltv pccpnltkmr vswpdirdal ldfvtrpseg vrgyvkddrg tlvnhallti
      361 rgrpvgfwss dqgefwrill pgdymvvasa pgylpaaarf dvregkrwqq pvvvtlhaap
      421 rtfpvlqipv svttyrsknk taggqrprpk pgsntgmpss strpkyllcl flapvwakvt
//
LOCUS       XP_077492917             236 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104007 [Amblyomma americanum].
ACCESSION   XP_077492917
VERSION     XP_077492917.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636791.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..236
                     /product="uncharacterized protein LOC144104007"
                     /calculated_mol_wt=27151
     Region          <1..44
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     CDS             1..236
                     /gene="LOC144104007"
                     /coded_by="XM_077636791.1:1..711"
                     /db_xref="GeneID:144104007"
ORIGIN      
        1 meinvsktkt mtftrasnvh lssyfmnsmc ienvytlkyl gvhltsnltw ndhideiisk
       61 anktlgfikr nlylanqstk llaytalvrs kieyasiiwn pnqtylinkl eslqnkaarv
      121 itktysrtst itaiknslql psletrrlla llshfhrlyh apssftashi kppqkifprl
      181 dhpfkvrpmf artnllrqsp lflaihhwnk lpkeiasird hdsfvsnlkr sfthvg
//
LOCUS       XP_077492946             444 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like [Amblyomma americanum].
ACCESSION   XP_077492946
VERSION     XP_077492946.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636820.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..444
                     /product="tubulin beta chain-like"
                     /calculated_mol_wt=49618
     Region          1..439
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..444
                     /gene="LOC144104035"
                     /coded_by="XM_077636820.1:30..1364"
                     /db_xref="GeneID:144104035"
ORIGIN      
        1 mreiiqiqtg qcgnqigskf weviseehgi drqgsyhgds dlqlerinvy yneasggkyv
       61 prgilvdlep gtmnavrsgp fgelfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaencdcl qgfqlthslg ggtgsgmgtl liskireeyp drinnsfsvv pspkvsdtvv
      181 epynatlsmh qllentdetf cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmpgf apltargsqq ysaltvpelt qqlfdaknmm
      301 aacdprqgry ltvaaifrgr ismrdvdeqm lhvqnknasy fvewipnnik tavcdipprg
      361 fkmsatlian staiqelfkr iseqftamfr rkaflhwytg egmdemefse aesnmndlis
      421 eyqqyqeatv ddegefddep vvqa
//
LOCUS       XP_077492960             129 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104048 [Amblyomma americanum].
ACCESSION   XP_077492960
VERSION     XP_077492960.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636834.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..129
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..129
                     /product="uncharacterized protein LOC144104048"
                     /calculated_mol_wt=13681
     CDS             1..129
                     /gene="LOC144104048"
                     /coded_by="XM_077636834.1:641..1030"
                     /db_xref="GeneID:144104048"
ORIGIN      
        1 mpscratars tarastqdgr vvaamrlyee padpaaeese nipryrdarg cmdavavleg
       61 mytrsqspaa ssqlelpmet pgslyqrmtp eeegspaqps hsfnqqspsd prslgipvts
      121 ssppgrrgg
//
LOCUS       XP_077492972             197 aa            linear   INV 25-NOV-2025
DEFINITION  homeobox protein Nkx-3.2-like [Amblyomma americanum].
ACCESSION   XP_077492972
VERSION     XP_077492972.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636846.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..197
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..197
                     /product="homeobox protein Nkx-3.2-like"
                     /calculated_mol_wt=22399
     Region          59..115
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..197
                     /gene="LOC144104057"
                     /coded_by="XM_077636846.1:95..688"
                     /db_xref="GeneID:144104057"
ORIGIN      
        1 mdeapgfsic hllnesqrgl dlskpkrqdv eahrrgredr perchrnael qagdkakkkr
       61 craafshaqv felerrfshq ryltgperad lahalkltet qvkiwfqnrr yktkrrhqag
      121 gtqdttalpl tdngspicqr tatvkvlvrd dqlvyrpeel aprptllfps vslpglqqpf
      181 pasferrltc tcvgcat
//
LOCUS       XP_077492983              89 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077492983
VERSION     XP_077492983.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636857.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..89
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..89
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=9547
     Region          <2..45
                     /region_name="Piwi-like"
                     /note="PIWI domain. Domain found in proteins involved in
                     RNA silencing. RNA silencing refers to a group of related
                     gene-silencing mechanisms mediated by short RNA molecules,
                     including siRNAs, miRNAs, and heterochromatin-related
                     guide RNAs. The central component...; cl00628"
                     /db_xref="CDD:412485"
     CDS             1..89
                     /gene="LOC144104066"
                     /coded_by="XM_077636857.1:259..528"
                     /db_xref="GeneID:144104066"
ORIGIN      
        1 mfsaddlqkl sfylchtysr carsvsipap vyyahlaafr akhhiaskle tsgavsqsse
       61 gggdtvltta qyveavkvlq glqasmyfv
//
LOCUS       XP_077493005             454 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: delta-1-pyrroline-5-carboxylate synthase-like
            [Amblyomma americanum].
ACCESSION   XP_077493005
VERSION     XP_077493005.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636879.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..454
                     /product="LOW QUALITY PROTEIN:
                     delta-1-pyrroline-5-carboxylate synthase-like"
                     /calculated_mol_wt=48539
     Region          27..431
                     /region_name="ALDH_F18-19_ProA-GPR"
                     /note="Gamma-glutamyl phosphate reductase (GPR), aldehyde
                     dehydrogenase families 18 and 19; cd07079"
                     /db_xref="CDD:143398"
     Site            276
                     /site_type="active"
                     /note="putative catalytic cysteine [active]"
                     /db_xref="CDD:143398"
     CDS             1..454
                     /gene="LOC144104081"
                     /coded_by="XM_077636879.1:126..1490"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:144104081"
ORIGIN      
        1 maitrivqgk rvgtfftehq skmislesta anarkasrtl esltatqcsa avlamadale
       61 artadimean erdltvareq glsapmlarl altpaklsal adglrtiakt spsmlgrvlr
      121 ktrvaegmel sqvtvligvl lvifkprpds lpqsaalava tanglllkgg heaahtnrcf
      181 wqllqqclkp ydaadalalv stredvdell nlkgytdlvi prgsnqlvpd iqrksrgipv
      241 lghsdgichv yvdreadkqn amrivvdskc dypaacname tllvdrdhve ggllsalcsa
      301 lkahgvslys gprmaqlltf gppaapsmri eysgpqcave vgdsvdeale hinthgssht
      361 daivtenkdt aeqflrgads acvfhncstr fadgyrfglg aevgirtgri hargpvgmeg
      421 llttkrllrg sgnvvqdfge sgtktylhen lpss
//
LOCUS       XP_077493058             701 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104115 [Amblyomma americanum].
ACCESSION   XP_077493058
VERSION     XP_077493058.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636932.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..701
                     /product="uncharacterized protein LOC144104115"
                     /calculated_mol_wt=76814
     CDS             1..701
                     /gene="LOC144104115"
                     /coded_by="XM_077636932.1:487..2592"
                     /db_xref="GeneID:144104115"
ORIGIN      
        1 mpggakkhrs lhhfgrtrkk grgrkragaa talrehvmdn raptspdnav vpedaspdsv
       61 qdvesgrasi ntdviseevr eigspdgmrs vdlgharmst dlignreahe vassdsvrcv
      121 dsgrvhisfd aigdrevrea larektalqr lssipatark vepfaeasna ttapvpdqra
      181 apyvimnids lssllqvlrc etcnqpariv rgdsdyglav klivvcdncg diatewsspr
      241 vdgsktcnaf dvnllatrim vstgngqtam ndifaamgvs hrgmhhktfq rhlkqslapa
      301 atqvaevams qcaqkvatly edlcfghrgn iavsydgswm trghsshlcv gavielfsgy
      361 vldyvvlsnf clgcevgpkp dsegyaewka thqcqknsds kaghmeveag lilfrrsler
      421 hglhyttilc dgdsrtftai qeakvygfid vqkedcvnhv qkrmgtalrn lvqkqkgegk
      481 rslggrgrlt ndlimrlsty ygwalksndg dvdamqkavm atyrhvtstd ehsdhslcpt
      541 gdnswcrqna arakgepepg hhynlpkdva eamlpvytrl sertllqrcq rgktqnanes
      601 lhsviwslvs keqhsslvav qaavaeavlr fntgnlhass ailqqldtni tssgfqrvre
      661 kdlrrsassk kkreaslevr kqakkrhrdg ihpdyapgaf p
//
LOCUS       XP_077493072             103 aa            linear   INV 25-NOV-2025
DEFINITION  histone H4-like [Amblyomma americanum].
ACCESSION   XP_077493072
VERSION     XP_077493072.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636946.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..103
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..103
                     /product="histone H4-like"
                     /calculated_mol_wt=11199
     Region          18..101
                     /region_name="PTZ00015"
                     /note="histone H4; Provisional"
                     /db_xref="CDD:185397"
     CDS             1..103
                     /gene="LOC144104119"
                     /coded_by="XM_077636946.1:622..933"
                     /db_xref="GeneID:144104119"
ORIGIN      
        1 msgrgkggkg lgkggakrhs kvlrdniqgi tksairrlar rggvqrisgl iyeetrgvlk
       61 vflenvirda ltytehakrk tvtamdvvya lkrrgrtlyg fgg
//
LOCUS       XP_077493102             136 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104141 [Amblyomma americanum].
ACCESSION   XP_077493102
VERSION     XP_077493102.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077636976.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..136
                     /product="uncharacterized protein LOC144104141"
                     /calculated_mol_wt=14714
     CDS             1..136
                     /gene="LOC144104141"
                     /coded_by="XM_077636976.1:19..429"
                     /db_xref="GeneID:144104141"
ORIGIN      
        1 mpeqvaspeg tsvlqdasrl rtvavgsssp psrtgwwwpp gsglcligrk sqlggsstgp
       61 pllgelrgdg dahridvrmr rsdlrspqip hhrsgrlpqq hgqqvtrrsa arspllrrfi
      121 yaiaidlsvv fplplk
//
LOCUS       XP_077493132             148 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104158 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077493132
VERSION     XP_077493132.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637006.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="uncharacterized protein LOC144104158 isoform X1"
                     /calculated_mol_wt=16769
     CDS             1..148
                     /gene="LOC144104158"
                     /coded_by="XM_077637006.1:399..845"
                     /db_xref="GeneID:144104158"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedrrr rhlnrrltat mkrlsgvhvl
       61 nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiapasrar pgevyvvhrc
      121 rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077493141              98 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104158 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077493141
VERSION     XP_077493141.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637015.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..98
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..98
                     /product="uncharacterized protein LOC144104158 isoform X2"
                     /calculated_mol_wt=10614
     CDS             1..98
                     /gene="LOC144104158"
                     /coded_by="XM_077637015.1:478..774"
                     /db_xref="GeneID:144104158"
ORIGIN      
        1 mkrlsgvhvl nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiapasrar
       61 pgevyvvhrc rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077493161             148 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104173 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077493161
VERSION     XP_077493161.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637035.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="uncharacterized protein LOC144104173 isoform X1"
                     /calculated_mol_wt=16759
     CDS             1..148
                     /gene="LOC144104173"
                     /coded_by="XM_077637035.1:433..879"
                     /db_xref="GeneID:144104173"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedrrr rhlnrrltat mkrlsgvhvl
       61 nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar pgevyvvhrc
      121 rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077493170              98 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104173 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077493170
VERSION     XP_077493170.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637044.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..98
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..98
                     /product="uncharacterized protein LOC144104173 isoform X2"
                     /calculated_mol_wt=10604
     CDS             1..98
                     /gene="LOC144104173"
                     /coded_by="XM_077637044.1:512..808"
                     /db_xref="GeneID:144104173"
ORIGIN      
        1 mkrlsgvhvl nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar
       61 pgevyvvhrc rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077493182             192 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104186 [Amblyomma americanum].
ACCESSION   XP_077493182
VERSION     XP_077493182.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637056.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..192
                     /product="uncharacterized protein LOC144104186"
                     /calculated_mol_wt=21564
     CDS             1..192
                     /gene="LOC144104186"
                     /coded_by="XM_077637056.1:383..961"
                     /db_xref="GeneID:144104186"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedrrr rhlnrrltat mkrlsgvhvl
       61 nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar pgevyvvhrc
      121 rrcgakghkt dhcgptahra atplqvavev llqtalfrat slfpgrsras rfvpvfplcr
      181 nrrqtsksrg vs
//
LOCUS       XP_077493195             148 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104194 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077493195
VERSION     XP_077493195.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637069.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="uncharacterized protein LOC144104194 isoform X1"
                     /calculated_mol_wt=16759
     CDS             1..148
                     /gene="LOC144104194"
                     /coded_by="XM_077637069.1:251..697"
                     /db_xref="GeneID:144104194"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedrrr rhlnrrltat mkrlsgvhvl
       61 nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar pgevyvvhrc
      121 rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077493203              98 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104194 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077493203
VERSION     XP_077493203.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637077.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..98
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..98
                     /product="uncharacterized protein LOC144104194 isoform X2"
                     /calculated_mol_wt=10604
     CDS             1..98
                     /gene="LOC144104194"
                     /coded_by="XM_077637077.1:333..629"
                     /db_xref="GeneID:144104194"
ORIGIN      
        1 mkrlsgvhvl nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar
       61 pgevyvvhrc rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077493208              98 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104194 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077493208
VERSION     XP_077493208.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637082.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..98
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..98
                     /product="uncharacterized protein LOC144104194 isoform X2"
                     /calculated_mol_wt=10604
     CDS             1..98
                     /gene="LOC144104194"
                     /coded_by="XM_077637082.1:314..610"
                     /db_xref="GeneID:144104194"
ORIGIN      
        1 mkrlsgvhvl nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar
       61 pgevyvvhrc rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077493298             787 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104272 [Amblyomma americanum].
ACCESSION   XP_077493298
VERSION     XP_077493298.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637172.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..787
                     /product="uncharacterized protein LOC144104272"
                     /calculated_mol_wt=87255
     Region          <234..485
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     Region          319..342
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          343..364
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          365..402
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          403..428
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     CDS             1..787
                     /gene="LOC144104272"
                     /coded_by="XM_077637172.1:16..2379"
                     /db_xref="GeneID:144104272"
ORIGIN      
        1 masqkraaet sgaasirysa dmapgapdvq ddqgspaalp aasrpdrppp apspnahwtl
       61 ssfakhlhqe apsffpsada vlkcvraaev elraagvems rpctfsesgr dpcwvhyhrd
      121 swndvlvhyg pelleasggr tmslrtlwlp graqlesles ltsaclvlwl lyehrcitsa
      181 tlnagviaph hvalfyrllr fhggyigagv qadhaepagv wrcrvfdalq ctsrlhtlhl
      241 salrlnepqs dslcaviarn pslsvlvlrd vvvdmiaavn lfngltflkm ldelefeatv
      301 deeqgvydea vaillhttvr rlkltlacgv teffgqlaan hrlqelelgh pvsdaqsfta
      361 latslvtnrv lsclkisvrv dcergyddfc qalaeivakn rrlevldlsg ttfhgdraiq
      421 alsdslrqnc tlnevhlhds dltctdvlrl ldglatndtv remcfgavkd spaaralvlk
      481 reeikdlmtr atfvwtddei hnlstrisrf slrkfylrgq dawpedvgql lsllpefqrs
      541 lntlyieasg aitvegadcl stlfeessvl rkvavlydtt qesslalleg lagstsittf
      601 afgrwylggm vsvafrtalm rnksllqldv hrfegecvhp efdarfedav atstslvavr
      661 rfrdgrevra qtlhsgmrsa lrnnetalrh tldvivgkgl aaqayyaynk lrscdsrgsy
      721 fsdcpaswpm catlqeiynt vrdplqlfsl efeslfdddq erdlraefqh lyhecrrqie
      781 ghlssav
//
LOCUS       XP_077493368             174 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104316 [Amblyomma americanum].
ACCESSION   XP_077493368
VERSION     XP_077493368.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637242.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..174
                     /product="uncharacterized protein LOC144104316"
                     /calculated_mol_wt=20089
     CDS             1..174
                     /gene="LOC144104316"
                     /coded_by="XM_077637242.1:21..545"
                     /db_xref="GeneID:144104316"
ORIGIN      
        1 myvlvryage drsyilqhtd vrdfvphdie dydpkkvydi fwagdsstcg gyydgkiihm
       61 tdteeemeqw rkrkearkca rktdipprkk arleeqakgv akknaerell ssrvgnspee
      121 skvahleslv rdlkaqlerc kevigdkeke mknlrslnes lqkalcakil rngm
//
LOCUS       XP_077493378             198 aa            linear   INV 25-NOV-2025
DEFINITION  BEN domain-containing protein 5-like [Amblyomma americanum].
ACCESSION   XP_077493378
VERSION     XP_077493378.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637252.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..198
                     /product="BEN domain-containing protein 5-like"
                     /calculated_mol_wt=21036
     CDS             1..198
                     /gene="LOC144104322"
                     /coded_by="XM_077637252.1:82..678"
                     /db_xref="GeneID:144104322"
ORIGIN      
        1 mhshslntia pvlpldvasp laappvappr advappradv appradvapp radvtpavpp
       61 arpmskaprk aaqdldlnvg ttnetgevhv ghghyidpgt werllaannd safckqlavr
      121 lwrntvlseg sgtgtlsnna iskgatkvfp plspakvasm adsfwhflkl kgclgdemlk
      181 rhkrlpcyla ekcsdlrr
//
LOCUS       XP_077493396             155 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104333 [Amblyomma americanum].
ACCESSION   XP_077493396
VERSION     XP_077493396.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637270.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..155
                     /product="uncharacterized protein LOC144104333"
                     /calculated_mol_wt=17229
     CDS             1..155
                     /gene="LOC144104333"
                     /coded_by="XM_077637270.1:37..504"
                     /db_xref="GeneID:144104333"
ORIGIN      
        1 mhprppclqg ipckaswdah ylatrrlssv rdkvpspste hgarhlygas crargarrit
       61 sisdsvresc itpsltvkst nnrqryhrhr panfqqgafp whwqqrqqpa htspelllhv
      121 faglvqvaaa allcgapvak rnrdpvppky vyqlg
//
LOCUS       XP_077493418             314 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104345 [Amblyomma americanum].
ACCESSION   XP_077493418
VERSION     XP_077493418.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637292.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..314
                     /product="uncharacterized protein LOC144104345"
                     /calculated_mol_wt=34544
     Region          183..>296
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(241,280,294,296)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..314
                     /gene="LOC144104345"
                     /coded_by="XM_077637292.1:104..1048"
                     /db_xref="GeneID:144104345"
ORIGIN      
        1 mngtvlvahc tcmagtgetc shvgaclfaa etavrirnsv sctqkeniwl payvekvqfk
       61 rlrdinftss rgrkeqidgt raegqqkrrr leipgpspee lnalyddigk dgavpalfsv
      121 lddrcdvfqe pvkkpashlr nlftddalgd sydvlvnkar elmstltise gtvkeveslt
      181 kqqsdsphwf syragritvs lmkkachtka anpsvslike icypeaqtmk vpaimwgrnh
      241 ekdalaayan segtkhdnfk lsksglhlst rypfsgatpd alvscsccrk gvaefknaht
      301 clgmqlhslm ktra
//
LOCUS       XP_077493440             220 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104361 [Amblyomma americanum].
ACCESSION   XP_077493440
VERSION     XP_077493440.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637314.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..220
                     /product="uncharacterized protein LOC144104361"
                     /calculated_mol_wt=23800
     CDS             1..220
                     /gene="LOC144104361"
                     /coded_by="XM_077637314.1:88..750"
                     /db_xref="GeneID:144104361"
ORIGIN      
        1 mhgaslnvts seldvpaeat aqatthvste taqvaparft gtedfvmhia elftslkamp
       61 lqrppstian asiayvagfv araveerrtc sfcpplhqsd gpspvlmgli dlqssggltf
      121 pksefvtilv tvkkavdial phikksnvrq qlaelllpll eqcplffcpa rndhaartlf
      181 vvfdkfmrpl lanvgaavtd raayrkklac kplhrkvlrv
//
LOCUS       XP_077493458             440 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104371 [Amblyomma americanum].
ACCESSION   XP_077493458
VERSION     XP_077493458.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637332.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..440
                     /product="uncharacterized protein LOC144104371"
                     /calculated_mol_wt=48997
     Region          224..426
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(280,319,340,342)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..440
                     /gene="LOC144104371"
                     /coded_by="XM_077637332.1:17..1339"
                     /db_xref="GeneID:144104371"
ORIGIN      
        1 maavddtahi vdgccecpag klacnhllav lrtimllqsr gfteapdsla ctdlpqqwrv
       61 prgsavkgcs vqsvdwrsvk eggrstpria lpkerrvqrr trpqqkdakr rlaldllacd
      121 pdkdfakgll ltdegesrqs rfgvvsslsp qsyqmslvph gfdvlssgle naalttveni
      181 pawkcfeett awlppaylng nsivqeiivt teaakalern trqqaksaaw qkerklrlts
      241 skfghvmkrk spwttkglen itasrelsrv pavnygvkme avaaqryedv lrkmghapvv
      301 tscgllvnpd fpwlgaspdm vgclasivyd svegsygave ikcpyslrdc kahellaadf
      361 csslidnvph lkrdhdyfyq fsgqmgisql gwgdfvvfgr hfilierirf eptewknvrr
      421 vldefyfttl lpylsglslr
//
LOCUS       XP_077493477             113 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104383 [Amblyomma americanum].
ACCESSION   XP_077493477
VERSION     XP_077493477.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637351.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..113
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..113
                     /product="uncharacterized protein LOC144104383"
                     /calculated_mol_wt=12223
     CDS             1..113
                     /gene="LOC144104383"
                     /coded_by="XM_077637351.1:31..372"
                     /db_xref="GeneID:144104383"
ORIGIN      
        1 mraprrstra araddfaprq avgscqtrrk vrllpplttk tlrcpaavfl gvgrarqdkn
       61 raslstrfrk iqseggsglr pldqakktgs qqngpayvqn iscpstsigc rrs
//
LOCUS       XP_077493519             146 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104406 [Amblyomma americanum].
ACCESSION   XP_077493519
VERSION     XP_077493519.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637393.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..146
                     /product="uncharacterized protein LOC144104406"
                     /calculated_mol_wt=16204
     CDS             1..146
                     /gene="LOC144104406"
                     /coded_by="XM_077637393.1:114..554"
                     /db_xref="GeneID:144104406"
ORIGIN      
        1 mhvavrvlcq kattprsmep fhldntfdfn rgsccrfflp rskrrkrllq psslrfqvkc
       61 llfsmkrsek sfneedvpls rfgavlfcar sayiakhkgt gggslagprd rllaiigrsv
      121 aptrpspfme avseraagtd slsrlv
//
LOCUS       XP_077493537             194 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104422 [Amblyomma americanum].
ACCESSION   XP_077493537
VERSION     XP_077493537.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637411.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..194
                     /product="uncharacterized protein LOC144104422"
                     /calculated_mol_wt=22013
     CDS             1..194
                     /gene="LOC144104422"
                     /coded_by="XM_077637411.1:1551..2135"
                     /db_xref="GeneID:144104422"
ORIGIN      
        1 mhlciafiel fnlkhkffsl gldlvnglsv eyipsvlqgv vkqltdtims tsnsaqrfyi
       61 gapsavsrin arlltikpph gitrlpravs eraqwkasew rhwilfyalr cldgilhady
      121 wrhlaklsea lhillresit fseidkaesl lehfvirchs lygigsitfn mhallhlanc
      181 vrsltfvgpf slrf
//
LOCUS       XP_077493562             109 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104440 [Amblyomma americanum].
ACCESSION   XP_077493562
VERSION     XP_077493562.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637436.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..109
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..109
                     /product="uncharacterized protein LOC144104440"
                     /calculated_mol_wt=11126
     CDS             1..109
                     /gene="LOC144104440"
                     /coded_by="XM_077637436.1:192..521"
                     /db_xref="GeneID:144104440"
ORIGIN      
        1 mqsalfgvpt tdhassaaag eagvprpvgc grrldrnmks svsaswsypa aahtehsslv
       61 esedtqpkvh acqkreeqct cgsarnlaqp stviaatigl gaqapcppl
//
LOCUS       XP_077493572             146 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104444 [Amblyomma americanum].
ACCESSION   XP_077493572
VERSION     XP_077493572.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637446.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..146
                     /product="uncharacterized protein LOC144104444"
                     /calculated_mol_wt=15820
     CDS             1..146
                     /gene="LOC144104444"
                     /coded_by="XM_077637446.1:281..721"
                     /db_xref="GeneID:144104444"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrppfa altsvlccqr pasigpcvlp afgcvippdp
       61 fmlktkscme vvqatklrvi fardleqagk ladgqwysls kasffsktkt iflgswvmyl
      121 gtcgqdnagq hasassekkh etvnky
//
LOCUS       XP_077493584             148 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104453 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077493584
VERSION     XP_077493584.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637458.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="uncharacterized protein LOC144104453 isoform X1"
                     /calculated_mol_wt=16803
     CDS             1..148
                     /gene="LOC144104453"
                     /coded_by="XM_077637458.1:438..884"
                     /db_xref="GeneID:144104453"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedrrr rhlnrrltat mkrlsgvhvl
       61 nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasdsrar pgevyvvhrc
      121 rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077493593              98 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104453 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077493593
VERSION     XP_077493593.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637467.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..98
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..98
                     /product="uncharacterized protein LOC144104453 isoform X2"
                     /calculated_mol_wt=10648
     CDS             1..98
                     /gene="LOC144104453"
                     /coded_by="XM_077637467.1:517..813"
                     /db_xref="GeneID:144104453"
ORIGIN      
        1 mkrlsgvhvl nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasdsrar
       61 pgevyvvhrc rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077493682             156 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104494 [Amblyomma americanum].
ACCESSION   XP_077493682
VERSION     XP_077493682.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637556.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..156
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..156
                     /product="uncharacterized protein LOC144104494"
                     /calculated_mol_wt=17034
     CDS             1..156
                     /gene="LOC144104494"
                     /coded_by="XM_077637556.1:266..736"
                     /db_xref="GeneID:144104494"
ORIGIN      
        1 mvtrptsgny rqhsrepgpn gvpgasstpa yqhnsapgps etiieleewr ldgalqaslv
       61 hkvladeplp srheslpsrh espassqpvp slqapvslle qeasvlqssa ptenkrkrrk
      121 ecaddtlaeh vgklqmhcrp vrdmtsttsf vfqwpk
//
LOCUS       XP_077493691             156 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104494 [Amblyomma americanum].
ACCESSION   XP_077493691
VERSION     XP_077493691.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637565.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..156
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..156
                     /product="uncharacterized protein LOC144104494"
                     /calculated_mol_wt=17034
     CDS             1..156
                     /gene="LOC144104494"
                     /coded_by="XM_077637565.1:411..881"
                     /db_xref="GeneID:144104494"
ORIGIN      
        1 mvtrptsgny rqhsrepgpn gvpgasstpa yqhnsapgps etiieleewr ldgalqaslv
       61 hkvladeplp srheslpsrh espassqpvp slqapvslle qeasvlqssa ptenkrkrrk
      121 ecaddtlaeh vgklqmhcrp vrdmtsttsf vfqwpk
//
LOCUS       XP_077493704             116 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104505 [Amblyomma americanum].
ACCESSION   XP_077493704
VERSION     XP_077493704.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637578.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..116
                     /product="uncharacterized protein LOC144104505"
                     /calculated_mol_wt=12868
     CDS             1..116
                     /gene="LOC144104505"
                     /coded_by="XM_077637578.1:77..427"
                     /db_xref="GeneID:144104505"
ORIGIN      
        1 mptadsmeeh mrdffavsaf phavgaldgc hfpvsppeeh asdyinykgc ylssgqvicn
       61 valafrvdie ttrrcihetc qailerlkdh fmqanmgrnc qrfcpamavs ellrsg
//
LOCUS       XP_077493747             138 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104539 [Amblyomma americanum].
ACCESSION   XP_077493747
VERSION     XP_077493747.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637621.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..138
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..138
                     /product="uncharacterized protein LOC144104539"
                     /calculated_mol_wt=15253
     Region          63..>120
                     /region_name="BEN"
                     /note="The BEN domain is found in diverse animal proteins;
                     smart01025"
                     /db_xref="CDD:214981"
     CDS             1..138
                     /gene="LOC144104539"
                     /coded_by="XM_077637621.1:52..468"
                     /db_xref="GeneID:144104539"
ORIGIN      
        1 msflflllav pakmvkrler mleskkepdq pvvpcakvdi gngvlvdrti lqmlerqcqs
       61 spgkfarall rnvfteeelr gkslygmknn vqkglpvkea ldpvrlsavi gytcdhfpgv
      121 nvaylknsla allareik
//
LOCUS       XP_077493799             353 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104573 [Amblyomma americanum].
ACCESSION   XP_077493799
VERSION     XP_077493799.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637673.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..353
                     /product="uncharacterized protein LOC144104573"
                     /calculated_mol_wt=38263
     CDS             1..353
                     /gene="LOC144104573"
                     /coded_by="XM_077637673.1:16..1077"
                     /db_xref="GeneID:144104573"
ORIGIN      
        1 maelpcgpcg sdadrqgdmg dhrtceqsep kvgdrlesgi ysggdtvqel epagstalpg
       61 fedeqqeast aksqpggaed apfstvsdeg hgdalvdpev iehyllevyy gplvtssgtv
      121 smflkhhvrv disvdravrv vnfakhcmaa fsrfgdrscv chpcgralqe gctvdvetgk
      181 rlakisrrgv tftafhgrhl hglvylvdns gtkstterfk vlrydlalnv frcgseqgsh
      241 linscfeiva qsiyesteng dfwlvggvhi iqrpsgdvev spdygrnvif tsptdgsism
      301 ntpiskiivs ccpdrflfvs mgrkrlcvsa erfvvrnggq kagldsmsrl slr
//
LOCUS       XP_077493809             342 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104581 [Amblyomma americanum].
ACCESSION   XP_077493809
VERSION     XP_077493809.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637683.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..342
                     /product="uncharacterized protein LOC144104581"
                     /calculated_mol_wt=37080
     CDS             1..342
                     /gene="LOC144104581"
                     /coded_by="XM_077637683.1:135..1163"
                     /db_xref="GeneID:144104581"
ORIGIN      
        1 mgdslpsfer aeqskttlpg gcsshfdgnt pkepepacyp pvtalmgned keegdsasqp
       61 qpsaaeeaeq fsldsaqgig dtpvdpaave qylleeyygp lvtssgtvsm flkkrvrvdi
      121 svdravrvvn fskhcmaals rlgdricich pcgrvlqegp tinietgmrl akmsrrgvtf
      181 talhghhlrg lvylvdnsgt ksttemfkeq sydvalkvfr sesdqgiqti nkcfeivaqv
      241 tqeptedged vwliggvciv qkssgdvevs pdfgrniifs spaegaisit tpvskmavsy
      301 cpdkflfvrm grkrvcvsae kfvvrnggqk agldsrgrlt lr
//
LOCUS       XP_077493833             218 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104599 [Amblyomma americanum].
ACCESSION   XP_077493833
VERSION     XP_077493833.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637707.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..218
                     /product="uncharacterized protein LOC144104599"
                     /calculated_mol_wt=23377
     CDS             1..218
                     /gene="LOC144104599"
                     /coded_by="XM_077637707.1:214..870"
                     /db_xref="GeneID:144104599"
ORIGIN      
        1 mdlidlivht llcptaqrrw mtrvhmhhct cnapmallct cpaavanqpr kqkqaaasas
       61 gsvvrlqrav gprgssilpa gaypptapkk drlhlkvaaa akagllvpph gggrvllyqc
      121 qqcchlfntp qglgmhksah rrvasalsqg lvcrhggggn rspakaalpq lgakkkneas
      181 qhpkwgirtm qntvrqrthd ecsthikegs lpmcdvkw
//
LOCUS       XP_077493845             513 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome P450 3A14-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077493845
VERSION     XP_077493845.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637719.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..513
                     /product="cytochrome P450 3A14-like isoform X1"
                     /calculated_mol_wt=58522
     Region          64..505
                     /region_name="cytochrome_P450"
                     /note="cytochrome P450 (CYP) superfamily; cl41757"
                     /db_xref="CDD:477761"
     Site            order(126,130,137,184,313..314,317..318,321..322,325,377,
                     387,389,412,448..450,454..458,461..462)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410651"
     Site            order(213..214,313,316..317,321,385..388)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410651"
     CDS             1..513
                     /gene="LOC144104608"
                     /coded_by="XM_077637719.1:22..1563"
                     /db_xref="GeneID:144104608"
ORIGIN      
        1 msatagllll vvlltalllt wrwrhfsyfr rlgipgprpn llwgnlmeyh stdlykvigr
       61 wiekygdmfg fyngdvpfvv tidldliehv cvrnfknfmn rgltmatvql hpylgksllh
      121 attphwrsir savaggftsa klkqmmpele edvvvflrsl kqyantgdqv hmlrlyepla
      181 mdlvgrrcfg teerfqgnpg hpliglakht tynamtgavh mvgqsitcfg pimrplcfls
      241 lvarrfrptr lqtekiiemr rkdpsyrkpd ylqnlletky vergtvssas qgdngipksr
      301 elttdevlts agtlclagfd titsslsyft ftlakhpavq ekvraeirea vreqgtldye
      361 tvmkrlnyle qvmnetlrly ppgltfvtrr aeqdfeykgi kfkagtcfmv plyqiqrdar
      421 fwpeplnfnp erfspenegs lnkrafvpfg igprncvgmk iavlymkftm aklvekyyfl
      481 lgpsqmgelr ldshamvsep sggpwiilhh anl
//
LOCUS       XP_077493854             463 aa            linear   INV 25-NOV-2025
DEFINITION  thromboxane-A synthase-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077493854
VERSION     XP_077493854.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637728.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..463
                     /product="thromboxane-A synthase-like isoform X2"
                     /calculated_mol_wt=52323
     Region          21..455
                     /region_name="cytochrome_P450"
                     /note="cytochrome P450 (CYP) superfamily; cl41757"
                     /db_xref="CDD:477761"
     Site            order(76,80,87,134,263..264,267..268,271..272,275,327,337,
                     339,362,398..400,404..408,411..412)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410651"
     Site            order(163..164,263,266..267,271,335..338)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410651"
     CDS             1..463
                     /gene="LOC144104608"
                     /coded_by="XM_077637728.1:25..1416"
                     /db_xref="GeneID:144104608"
ORIGIN      
        1 msatagllll vvlltalllt fyngdvpfvv tidldliehv cvrnfknfmn rgltmatvql
       61 hpylgksllh attphwrsir savaggftsa klkqmmpele edvvvflrsl kqyantgdqv
      121 hmlrlyepla mdlvgrrcfg teerfqgnpg hpliglakht tynamtgavh mvgqsitcfg
      181 pimrplcfls lvarrfrptr lqtekiiemr rkdpsyrkpd ylqnlletky vergtvssas
      241 qgdngipksr elttdevlts agtlclagfd titsslsyft ftlakhpavq ekvraeirea
      301 vreqgtldye tvmkrlnyle qvmnetlrly ppgltfvtrr aeqdfeykgi kfkagtcfmv
      361 plyqiqrdar fwpeplnfnp erfspenegs lnkrafvpfg igprncvgmk iavlymkftm
      421 aklvekyyfl lgpsqmgelr ldshamvsep sggpwiilhh anl
//
LOCUS       XP_077493861             446 aa            linear   INV 25-NOV-2025
DEFINITION  thromboxane-A synthase-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077493861
VERSION     XP_077493861.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637735.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..446
                     /product="thromboxane-A synthase-like isoform X3"
                     /calculated_mol_wt=50650
     Region          2..438
                     /region_name="cytochrome_P450"
                     /note="cytochrome P450 (CYP) superfamily; cl41757"
                     /db_xref="CDD:477761"
     Site            order(59,63,70,117,246..247,250..251,254..255,258,310,320,
                     322,345,381..383,387..391,394..395)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410651"
     Site            order(146..147,246,249..250,254,318..321)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410651"
     CDS             1..446
                     /gene="LOC144104608"
                     /coded_by="XM_077637735.1:126..1466"
                     /db_xref="GeneID:144104608"
ORIGIN      
        1 mfgfyngdvp fvvtidldli ehvcvrnfkn fmnrgltmat vqlhpylgks llhattphwr
       61 sirsavaggf tsaklkqmmp eleedvvvfl rslkqyantg dqvhmlrlye plamdlvgrr
      121 cfgteerfqg npghpligla khttynamtg avhmvgqsit cfgpimrplc flslvarrfr
      181 ptrlqtekii emrrkdpsyr kpdylqnlle tkyvergtvs sasqgdngip ksrelttdev
      241 ltsagtlcla gfdtitssls yftftlakhp avqekvraei reavreqgtl dyetvmkrln
      301 yleqvmnetl rlyppgltfv trraeqdfey kgikfkagtc fmvplyqiqr darfwpepln
      361 fnperfspen egslnkrafv pfgigprncv gmkiavlymk ftmaklveky yfllgpsqmg
      421 elrldshamv sepsggpwii lhhanl
//
LOCUS       XP_077493869             434 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome P450 3A14-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077493869
VERSION     XP_077493869.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637743.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..434
                     /product="cytochrome P450 3A14-like isoform X4"
                     /calculated_mol_wt=49714
     Region          64..432
                     /region_name="cytochrome_P450"
                     /note="cytochrome P450 (CYP) superfamily; cl41757"
                     /db_xref="CDD:477761"
     Site            order(213..214,313,316..317,321,385..388)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410651"
     CDS             1..434
                     /gene="LOC144104608"
                     /coded_by="XM_077637743.1:22..1326"
                     /db_xref="GeneID:144104608"
ORIGIN      
        1 msatagllll vvlltalllt wrwrhfsyfr rlgipgprpn llwgnlmeyh stdlykvigr
       61 wiekygdmfg fyngdvpfvv tidldliehv cvrnfknfmn rgltmatvql hpylgksllh
      121 attphwrsir savaggftsa klkqmmpele edvvvflrsl kqyantgdqv hmlrlyepla
      181 mdlvgrrcfg teerfqgnpg hpliglakht tynamtgavh mvgqsitcfg pimrplcfls
      241 lvarrfrptr lqtekiiemr rkdpsyrkpd ylqnlletky vergtvssas qgdngipksr
      301 elttdevlts agtlclagfd titsslsyft ftlakhpavq ekvraeirea vreqgtldye
      361 tvmkrlnyle qvmnetlrly ppgltfvtrr aeqdfeykgi kfkagtcfmv plyqiqrdar
      421 fwpeplnfnp ervn
//
LOCUS       XP_077493938             212 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104668 [Amblyomma americanum].
ACCESSION   XP_077493938
VERSION     XP_077493938.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637812.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..212
                     /product="uncharacterized protein LOC144104668"
                     /calculated_mol_wt=23412
     CDS             1..212
                     /gene="LOC144104668"
                     /coded_by="XM_077637812.1:55..693"
                     /db_xref="GeneID:144104668"
ORIGIN      
        1 mkcrwkkkks eenreifrtg ggtlecrpms patelvgava dhmatrlpnp fdsdgahvge
       61 avlslppaal leamvngnet sqddvpaqsp llnsrvined elpvqeecln asrrtvvvpn
      121 qsssegippt qtartraggp rvaavertla pelaaritai eaeerrkeel hqldvqlrrs
      181 qlaeqrlkvk mqrkllsldi qirkkqleam qr
//
LOCUS       XP_077493952             284 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104685 [Amblyomma americanum].
ACCESSION   XP_077493952
VERSION     XP_077493952.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637826.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..284
                     /product="uncharacterized protein LOC144104685"
                     /calculated_mol_wt=32269
     Region          42..134
                     /region_name="MADF_DNA_bdg"
                     /note="Alcohol dehydrogenase transcription factor
                     Myb/SANT-like; pfam10545"
                     /db_xref="CDD:463144"
     Region          247..280
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..284
                     /gene="LOC144104685"
                     /coded_by="XM_077637826.1:52..906"
                     /db_xref="GeneID:144104685"
ORIGIN      
        1 mrngeaevdf rnpsyptcps ahfvcvvvic ivrawrcrsw nvecrpvllq vkhkdhknrl
       61 kknilwadva aavlpnvpna enlmqkrwky lrdkfrrilv ankesqksga gaedaqesvn
      121 rdgswayydq imflrdgveg rptsgnmtaq vllqiivtgy cymepvsipp iensqgqagg
      181 aftteqmiak dmavapdnqs lldeppsltp pppapearpl qkkrkhesfr eeleqvsaqi
      241 rnitpqdvde hfamsllpnr rrvkperrie mqlrvvqvlq vfse
//
LOCUS       XP_077494033            1057 aa            linear   INV 25-NOV-2025
DEFINITION  telomerase-binding protein EST1A-like [Amblyomma americanum].
ACCESSION   XP_077494033
VERSION     XP_077494033.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637907.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1057
                     /product="telomerase-binding protein EST1A-like"
                     /calculated_mol_wt=119333
     Region          <36..174
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          266..385
                     /region_name="EST1"
                     /note="Telomerase activating protein Est1; pfam10374"
                     /db_xref="CDD:463062"
     Site            order(371,374..375,378..379,381,411,414..415,418..419,
                     421..422,445,448..449)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          371..404
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          395..739
                     /region_name="EST1_DNA_bind"
                     /note="Est1 DNA/RNA binding domain; pfam10373"
                     /db_xref="CDD:431239"
     Region          409..439
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          878..1054
                     /region_name="PIN_Smg6-like"
                     /note="VapC-like PIN domain of human telomerase-binding
                     protein EST1, Smg6, and other similar eukaryotic homologs;
                     cd09885"
                     /db_xref="CDD:350233"
     CDS             1..1057
                     /gene="LOC144104737"
                     /coded_by="XM_077637907.1:8..3181"
                     /db_xref="GeneID:144104737"
ORIGIN      
        1 mfsqkadnps sgasgvsasw hwmlmevrwk nlkneatsar kasppktpks qlpphyqkrn
       61 rptssenvva assaakgeln ripcnislvs slgissehre ersaasappc sagqdkplia
      121 akpqphlpvd nvhsetavpi tpqpsftcqf easppvvspa vetphnpaav ptvpfsgllp
      181 dhkvspvyhg nqlispssym ysrsrvkplv eknlheaarl yrelstylsk eiqnidtkam
      241 ghcrwklqlr yenviladpr lcaerniens lwksafyqgi etfrrvmdqc pdfreeakiq
      301 llnlvdegin fyenllykfe ethglslnqf fdaelcgllg tgsspslgvp daaggrslpd
      361 svrlvlisvq kiyiclgdla ryreqamgtt nygkarnwyl kaqqiapkng rpynqlaila
      421 vyarrkldav yyymrslaas npfltaresl lalfddarkt yehlerkrle edgtnqqrvl
      481 enpwdeerle iwirsdgfss hrsscrhadq asreleqlvk lstidlkkry tlsflhvhgk
      541 lfsrvgmetf patarrmlme frcllqrtpa aithtrhlql lainmftvan tslkvcslap
      601 dcqsllqers lqvalahvai mlervtqllq qgcsssspss tpessspgvs lsddlaellp
      661 alkvwtdwms cqkrlwmppp spceyntsvk gnvwttladl ltvllklnln dvtfykdpae
      721 drelvtlped atlagfvpll gapqeafyvq vpcnrerars ylrisriqlf geylcgistp
      781 ylefnverkr fvslitvsdi ksdskkevty dssdeelemg dslddqkaqf tsseeekeiq
      841 klwtkkeqlr rakeqherhh aciqavlqeh rskqafilei rprylipdtn sfidhlplvk
      901 klvadghfyv yvpivvlnel hglsrggsrp gtgyadhcge vssnaraalt yleerfgqre
      961 pklrtitlrg slldtinfrc edlgdykgtn ddqiltccql lcrdraeqaf prepdaplkl
     1021 yrdtvlited rnlcikalth nvpvrdlpaf lrwanis
//
LOCUS       XP_077494055             425 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104753 [Amblyomma americanum].
ACCESSION   XP_077494055
VERSION     XP_077494055.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637929.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..425
                     /product="uncharacterized protein LOC144104753"
                     /calculated_mol_wt=47717
     CDS             1..425
                     /gene="LOC144104753"
                     /coded_by="XM_077637929.1:1..1278"
                     /db_xref="GeneID:144104753"
ORIGIN      
        1 mlapwqrlda vktfvypaln fsmrcgllgk aewerldeal rplikrtlyl panasndyiy
       61 gsaaagtagi plaaelsdic ridsafklls spdaevrdla kravtevttk rlgrevtead
      121 vaaylsgete gdfraratql ksvwtearea srrlevtwem qedgphitca dvtlapkhrt
      181 kvvkklrsia taarnhslqq kpnqgkvmec vtadpssshf mrsglftrfc dwcflhrarf
      241 nllplngarr werasdqrcr acghnketlp hvlchcirqs aaytdrhnki vnrlkaaagk
      301 rftithenrp vgttnlmpdl vlargesaiv idvtcpfdnr rtafdvarei kvakyepvhq
      361 clrrkfqrvs ikaivvgalg swdpnndkvm krlcsrsylr lfkklavsdt iaasrevysr
      421 hvaak
//
LOCUS       XP_077494068             146 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104760 [Amblyomma americanum].
ACCESSION   XP_077494068
VERSION     XP_077494068.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637942.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..146
                     /product="uncharacterized protein LOC144104760"
                     /calculated_mol_wt=15937
     CDS             1..146
                     /gene="LOC144104760"
                     /coded_by="XM_077637942.1:329..769"
                     /db_xref="GeneID:144104760"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrppfa altsvlccqw pasigpcvlp afgcvippdp
       61 fmlktkscme vvqatklrvi ftrdleqagk lvngqwysls kasffsktkt ifldswamyl
      121 gtcgqdnagq hasasskkkh etvnky
//
LOCUS       XP_077494094             176 aa            linear   INV 25-NOV-2025
DEFINITION  BEN domain-containing protein 5-like [Amblyomma americanum].
ACCESSION   XP_077494094
VERSION     XP_077494094.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637968.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..176
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..176
                     /product="BEN domain-containing protein 5-like"
                     /calculated_mol_wt=19474
     CDS             1..176
                     /gene="LOC144104783"
                     /coded_by="XM_077637968.1:45..575"
                     /db_xref="GeneID:144104783"
ORIGIN      
        1 mlcdapssag dshgnetadq pleeghkemi ekpvalfeav dgqvpigpql wmpaakwiyv
       61 mkntsdakcc levarhlwtl seaaersltg qqcrfiataa rklpatpekv evmknclayf
      121 vdrhpvpelp kdhrvaavrk hlrsffteaa rqgkrvrgaa aprdtqtnil qqhpnq
//
LOCUS       XP_077494106             165 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104791 [Amblyomma americanum].
ACCESSION   XP_077494106
VERSION     XP_077494106.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637980.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..165
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..165
                     /product="uncharacterized protein LOC144104791"
                     /calculated_mol_wt=18979
     Region          <26..76
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..165
                     /gene="LOC144104791"
                     /coded_by="XM_077637980.1:410..907"
                     /db_xref="GeneID:144104791"
ORIGIN      
        1 mrpyarrdlq khrlrpsgre eyqqratfny rlsrarrvie nsfgimaarw rilrrpfras
       61 eettenicka cvvlhnfmms esalarsays ppgyadsedw qgnetpglwr tdgndlpamr
      121 gisrqgchsa rsamdvrdrl aqyfvtdgkv pwqeaivdra grkea
//
LOCUS       XP_077494113             165 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104791 [Amblyomma americanum].
ACCESSION   XP_077494113
VERSION     XP_077494113.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077637987.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..165
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..165
                     /product="uncharacterized protein LOC144104791"
                     /calculated_mol_wt=18979
     Region          <26..76
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..165
                     /gene="LOC144104791"
                     /coded_by="XM_077637987.1:271..768"
                     /db_xref="GeneID:144104791"
ORIGIN      
        1 mrpyarrdlq khrlrpsgre eyqqratfny rlsrarrvie nsfgimaarw rilrrpfras
       61 eettenicka cvvlhnfmms esalarsays ppgyadsedw qgnetpglwr tdgndlpamr
      121 gisrqgchsa rsamdvrdrl aqyfvtdgkv pwqeaivdra grkea
//
LOCUS       XP_077494143             122 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104816 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077494143
VERSION     XP_077494143.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638017.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..122
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..122
                     /product="uncharacterized protein LOC144104816 isoform X1"
                     /calculated_mol_wt=13315
     Region          <74..117
                     /region_name="INCENP_ARK-bind"
                     /note="Inner centromere protein, ARK binding region;
                     pfam03941"
                     /db_xref="CDD:461100"
     CDS             1..122
                     /gene="LOC144104816"
                     /coded_by="XM_077638017.1:294..662"
                     /db_xref="GeneID:144104816"
ORIGIN      
        1 msgsqfsfst aleelyervk phhekmaiik eeassaiakv lekisaaqse eperpledpg
       61 egpshgpskt pkppqkrgpt waqnpllett fymqakgtkd gsvifgpvpr pslddvfqnk
      121 kd
//
LOCUS       XP_077494156              75 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104816 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077494156
VERSION     XP_077494156.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638030.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..75
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..75
                     /product="uncharacterized protein LOC144104816 isoform X2"
                     /calculated_mol_wt=8070
     Region          <27..70
                     /region_name="INCENP_ARK-bind"
                     /note="Inner centromere protein, ARK binding region;
                     pfam03941"
                     /db_xref="CDD:461100"
     CDS             1..75
                     /gene="LOC144104816"
                     /coded_by="XM_077638030.1:294..521"
                     /db_xref="GeneID:144104816"
ORIGIN      
        1 msgeperple dpgegpshgp sktpkppqkr gptwaqnpll ettfymqakg tkdgsvifgp
       61 vprpslddvf qnkkd
//
LOCUS       XP_077494169             293 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104827 [Amblyomma americanum].
ACCESSION   XP_077494169
VERSION     XP_077494169.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638043.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..293
                     /product="uncharacterized protein LOC144104827"
                     /calculated_mol_wt=33679
     CDS             1..293
                     /gene="LOC144104827"
                     /coded_by="XM_077638043.1:222..1103"
                     /db_xref="GeneID:144104827"
ORIGIN      
        1 mtslpkeraq sraqdatrkt rqeknhrdsn ldlpalylak gslfgwisap gsdtssegsa
       61 gdfalgdetv defpyvkisr aemkkqraml anieqklkfv aknlteksvv mpevmlnkfl
      121 eveqikfemr kmqeenrrlk eevhrrdefi ranglelsga gaglviqqaf epfedtsvlq
      181 eqqqnaclpn nsqrqakdam rrkmlrdaqm eekkrkhkqr paeeehawpr enafsvdkpr
      241 arpmthatet edwehelrad dveslehdst ehldkvinrf kggireghle dpd
//
LOCUS       XP_077494192             216 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104851 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077494192
VERSION     XP_077494192.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638066.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..216
                     /product="uncharacterized protein LOC144104851 isoform X1"
                     /calculated_mol_wt=23158
     Region          34..>187
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..216
                     /gene="LOC144104851"
                     /coded_by="XM_077638066.1:111..761"
                     /db_xref="GeneID:144104851"
ORIGIN      
        1 mrqhnsqsga drrtcefeee laeifekahs inppyllgpg llrsvqdsqa epaaspqpgp
       61 srvtataspi pgssqpstsr gsttagdsqp pspsaqpspl qqqpgparkr prtdtsravl
      121 esagahleva eanrqqrhre rmalegrkva averrtaale rvaaalerre lvslcspiha
      181 pahpqlisqf ipqsvlqsal qalltimlsf nvsffv
//
LOCUS       XP_077494201             215 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104851 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077494201
VERSION     XP_077494201.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638075.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..215
                     /product="uncharacterized protein LOC144104851 isoform X2"
                     /calculated_mol_wt=23071
     CDS             1..215
                     /gene="LOC144104851"
                     /coded_by="XM_077638075.1:111..758"
                     /db_xref="GeneID:144104851"
ORIGIN      
        1 mrqhnsqsga drrtcefeee laeifekahs inppyllgpg llrsvqdqae paaspqpgps
       61 rvtataspip gssqpstsrg sttagdsqpp spsaqpsplq qqpgparkrp rtdtsravle
      121 sagahlevae anrqqrhrer malegrkvaa verrtaaler vaaalerrel vslcspihap
      181 ahpqlisqfi pqsvlqsalq alltimlsfn vsffv
//
LOCUS       XP_077494273              92 aa            linear   INV 25-NOV-2025
DEFINITION  defensin-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077494273
VERSION     XP_077494273.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638147.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..92
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..92
                     /product="defensin-like isoform X1"
                     /calculated_mol_wt=9568
     CDS             1..92
                     /gene="LOC144104912"
                     /coded_by="XM_077638147.1:18..296"
                     /db_xref="GeneID:144104912"
ORIGIN      
        1 mtsggsasak trtrskaetw vaagaalmal flfllmavlv sgslyaapes hhlrlrsgfg
       61 cpyneltcsr qcvsagkshg fcghdnvcec vd
//
LOCUS       XP_077494276              78 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104912 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077494276
VERSION     XP_077494276.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638150.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..78
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..78
                     /product="uncharacterized protein LOC144104912 isoform X2"
                     /calculated_mol_wt=7936
     CDS             1..78
                     /gene="LOC144104912"
                     /coded_by="XM_077638150.1:40..276"
                     /db_xref="GeneID:144104912"
ORIGIN      
        1 mtsggsasak trtrskaetw vaagaalmal flfllmavlv sgsgfgcpyn eltcsrqcvs
       61 agkshgfcgh dnvcecvd
//
LOCUS       XP_077494284             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104925 [Amblyomma americanum].
ACCESSION   XP_077494284
VERSION     XP_077494284.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638158.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144104925"
                     /calculated_mol_wt=15218
     CDS             1..141
                     /gene="LOC144104925"
                     /coded_by="XM_077638158.1:22..447"
                     /db_xref="GeneID:144104925"
ORIGIN      
        1 maaactrytl vgfsaaldwr pirfvslapp sticgacgll rpvaarfpcq hvlcescyel
       61 saqgdharvc pldrlqclde dvgfqemaad ellktkvrcw neqagcdavm pasistmnva
      121 itrphvpnar hlfsaptsth t
//
LOCUS       XP_077494300             279 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104940 [Amblyomma americanum].
ACCESSION   XP_077494300
VERSION     XP_077494300.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638174.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..279
                     /product="uncharacterized protein LOC144104940"
                     /calculated_mol_wt=30144
     CDS             1..279
                     /gene="LOC144104940"
                     /coded_by="XM_077638174.1:153..992"
                     /db_xref="GeneID:144104940"
ORIGIN      
        1 mppadwhgtl agyavklgsa frtchpeadv tafthvqtfd twqendstid taycvlvttt
       61 vlcgpevlqs aaavaevrtp afrppdvsna aftatgedff tvswerpggs fdyywmvvkd
      121 sdsaldgsen qrvgscasga llrpqqthit chhvksctna sltiythvng ppertsqgvt
      181 vheifiqgkd lhppknvtft glspsltrlq wisssstqgt tdaytvkicd afqvcnstdg
      241 vqkcvhqetp lpwlefvttp dteycvlvas talcgqdti
//
LOCUS       XP_077494330             271 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104977 [Amblyomma americanum].
ACCESSION   XP_077494330
VERSION     XP_077494330.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638204.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..271
                     /product="uncharacterized protein LOC144104977"
                     /calculated_mol_wt=29428
     CDS             1..271
                     /gene="LOC144104977"
                     /coded_by="XM_077638204.1:188..1003"
                     /db_xref="GeneID:144104977"
ORIGIN      
        1 mefesiagte ycvlvsssar crsgvvrspp asatfrtppf vppdvtnmtl edigvdsftv
       61 swerpkghfd ynwvavtdde gssgrsehfh ldscrngtil hpeqtritcg hleacnnmss
      121 nirthsngpp artsagvtlg gifipgaapg dfradvtats ntsvqivihv sdvksclldd
      181 crgycyrlyg gdqhsgitsl avtttdspll spwmalkvtl invhkaqnmw kfkflyfdtp
      241 ksslgrlgaa srtttvrrph csqpapwfsa r
//
LOCUS       XP_077494340              92 aa            linear   INV 25-NOV-2025
DEFINITION  defensin-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077494340
VERSION     XP_077494340.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638214.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..92
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..92
                     /product="defensin-like isoform X1"
                     /calculated_mol_wt=9568
     CDS             1..92
                     /gene="LOC144104989"
                     /coded_by="XM_077638214.1:18..296"
                     /db_xref="GeneID:144104989"
ORIGIN      
        1 mtsggsasak trtrskaetw vaagaalmal flfllmavlv sgslyaapes hhlrlrsgfg
       61 cpyneltcsr qcvsagkshg fcghdnvcec vd
//
LOCUS       XP_077494348              78 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144104989 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077494348
VERSION     XP_077494348.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638222.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..78
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..78
                     /product="uncharacterized protein LOC144104989 isoform X2"
                     /calculated_mol_wt=7936
     CDS             1..78
                     /gene="LOC144104989"
                     /coded_by="XM_077638222.1:40..276"
                     /db_xref="GeneID:144104989"
ORIGIN      
        1 mtsggsasak trtrskaetw vaagaalmal flfllmavlv sgsgfgcpyn eltcsrqcvs
       61 agkshgfcgh dnvcecvd
//
LOCUS       XP_077494357             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105006 [Amblyomma americanum].
ACCESSION   XP_077494357
VERSION     XP_077494357.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638231.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144105006"
                     /calculated_mol_wt=15218
     CDS             1..141
                     /gene="LOC144105006"
                     /coded_by="XM_077638231.1:22..447"
                     /db_xref="GeneID:144105006"
ORIGIN      
        1 maaactrytl vgfsaaldwr pirfvslapp sticgacgll rpvaarfpcq hvlcescyel
       61 saqgdharvc pldrlqclde dvgfqemaad ellktkvrcw neqagcdavm pasistmnva
      121 itrphvpnar hlfsaptsth t
//
LOCUS       XP_077494368             192 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105027 [Amblyomma americanum].
ACCESSION   XP_077494368
VERSION     XP_077494368.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638242.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..192
                     /product="uncharacterized protein LOC144105027"
                     /calculated_mol_wt=20654
     CDS             1..192
                     /gene="LOC144105027"
                     /coded_by="XM_077638242.1:457..1035"
                     /db_xref="GeneID:144105027"
ORIGIN      
        1 mppadwhgtl agyavklgsa frtchpeadv tafthvqtfd twqendstid taycvlvttt
       61 vlcgpevlqs aaavaevrtp afrppdvsna aftatgedff tvswerpggs fdyywmvvkd
      121 sdsaldgsen qrvgscasga llrpqqthit chhvksctna sltiythvng ppertsqgvt
      181 vheifiqgkg ml
//
LOCUS       XP_077494379             102 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105035 [Amblyomma americanum].
ACCESSION   XP_077494379
VERSION     XP_077494379.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638253.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..102
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..102
                     /product="uncharacterized protein LOC144105035"
                     /calculated_mol_wt=11528
     CDS             1..102
                     /gene="LOC144105035"
                     /coded_by="XM_077638253.1:4552..4860"
                     /db_xref="GeneID:144105035"
ORIGIN      
        1 mdvcsrrhph gssqislqsh weifmssmea kaftphgycc swpvystgpv vyndysqhac
       61 rpakeilmwl rtcspreddy sagsplqftv qlpycpdyec sa
//
LOCUS       XP_077494417             152 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105104 [Amblyomma americanum].
ACCESSION   XP_077494417
VERSION     XP_077494417.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638291.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..152
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..152
                     /product="uncharacterized protein LOC144105104"
                     /calculated_mol_wt=17080
     CDS             1..152
                     /gene="LOC144105104"
                     /coded_by="XM_077638291.1:41..499"
                     /db_xref="GeneID:144105104"
ORIGIN      
        1 mntespalss rmeeeppelp vvpsprrera ldcrcllltl clscsvavft ivvvyfyffp
       61 veeekgtrvc gdvsswgety lkgkfdtclg tsiceleplc eaqdtvtcns rvpwfggrce
      121 ccdwcllrtl acvdkdhdra sfqycqlick hl
//
LOCUS       XP_077494423             162 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105115 [Amblyomma americanum].
ACCESSION   XP_077494423
VERSION     XP_077494423.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638297.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..162
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..162
                     /product="uncharacterized protein LOC144105115"
                     /calculated_mol_wt=15486
     CDS             1..162
                     /gene="LOC144105115"
                     /coded_by="XM_077638297.1:116..604"
                     /db_xref="GeneID:144105115"
ORIGIN      
        1 mktmhlflat vacalllalp tqqqgllglm ataaimrrfr ggnqnensgg qsggqtplas
       61 hpepsiyglp sqavpfyagg ypgayggpma gglpaggfgg gfpggsaafa pganpstyta
      121 sfgglnfntg gfggapqast ssafapggfg glpaaggvpy gg
//
LOCUS       XP_077494436             231 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105134 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077494436
VERSION     XP_077494436.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638310.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..231
                     /product="uncharacterized protein LOC144105134 isoform X1"
                     /calculated_mol_wt=26132
     Region          23..118
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     Region          <127..>209
                     /region_name="PLN02226"
                     /note="2-oxoglutarate dehydrogenase E2 component"
                     /db_xref="CDD:177871"
     CDS             1..231
                     /gene="LOC144105134"
                     /coded_by="XM_077638310.1:167..862"
                     /db_xref="GeneID:144105134"
ORIGIN      
        1 mfvcytcfqh hdnmsrglfn evliievskr pllwdvkdnn frnkstkesl weevrdaira
       61 iddtvtveei iarwknlkdt yrrkikdekd gkksgsgata ktawphlkqm eflrdsmetr
      121 rsfcnldavs hvqvasaaae tshascspas pdskeveatp nefeciysvp rlpdepgpsc
      181 maatspevlp hrgnkrqkkr rqeveaadrd iqavrsaiaa hrpmvtphvr t
//
LOCUS       XP_077494441             153 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105134 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077494441
VERSION     XP_077494441.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638315.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..153
                     /product="uncharacterized protein LOC144105134 isoform X2"
                     /calculated_mol_wt=17878
     Region          23..118
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     CDS             1..153
                     /gene="LOC144105134"
                     /coded_by="XM_077638315.1:167..628"
                     /db_xref="GeneID:144105134"
ORIGIN      
        1 mfvcytcfqh hdnmsrglfn evliievskr pllwdvkdnn frnkstkesl weevrdaira
       61 iddtvtveei iarwknlkdt yrrkikdekd gkksgsgata ktawphlkqm eflrdsmetr
      121 repraggqrs srdeprqlqp siarlersrs nak
//
LOCUS       XP_077494449             241 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105157 [Amblyomma americanum].
ACCESSION   XP_077494449
VERSION     XP_077494449.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638323.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..241
                     /product="uncharacterized protein LOC144105157"
                     /calculated_mol_wt=27941
     Region          23..117
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     CDS             1..241
                     /gene="LOC144105157"
                     /coded_by="XM_077638323.1:70..795"
                     /db_xref="GeneID:144105157"
ORIGIN      
        1 mfvcytrfrh hdnmsrglfn evliievskr pllwdvkdnf rnkstkeslw eevrdairai
       61 ddtvtveeii arwknlkdty rrktkdekdg kksgsgatak tawphlkqme flrdsmetrr
      121 sfcnldavsh vqvasaaaet shascspasp gskeveaspn dlnacivsqa flmnqdhpvw
      181 qqlhqrfshi getsdrrkgg rkwrlltgtf rpcemqlqrt gqwlhrtygl kkrcyyhqdk
      241 k
//
LOCUS       XP_077494457              73 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105174 [Amblyomma americanum].
ACCESSION   XP_077494457
VERSION     XP_077494457.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638331.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..73
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..73
                     /product="uncharacterized protein LOC144105174"
                     /calculated_mol_wt=8126
     CDS             1..73
                     /gene="LOC144105174"
                     /coded_by="XM_077638331.1:388..609"
                     /db_xref="GeneID:144105174"
ORIGIN      
        1 mnqeypegaa islkfvqrqf dlnlpnesrq kdvkrsrtsv pskimnlvnk lglqvetvnp
       61 cgvrksdatq ehs
//
LOCUS       XP_077494518             160 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105249 [Amblyomma americanum].
ACCESSION   XP_077494518
VERSION     XP_077494518.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638392.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..160
                     /product="uncharacterized protein LOC144105249"
                     /calculated_mol_wt=16972
     CDS             1..160
                     /gene="LOC144105249"
                     /coded_by="XM_077638392.1:149..631"
                     /db_xref="GeneID:144105249"
ORIGIN      
        1 mstsspgqgn spwsaslppn klsldalfrg gvgtipvala psdggairln nqdavqtalr
       61 aitphflkic dvrqygrggv vcrstdqaci mdlltctafg sqslwcrrfs elgtlprhwi
      121 lptdlpsphs rvffvasgqh llghacflps avssllirsc
//
LOCUS       XP_077494559             146 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105289 [Amblyomma americanum].
ACCESSION   XP_077494559
VERSION     XP_077494559.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638433.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..146
                     /product="uncharacterized protein LOC144105289"
                     /calculated_mol_wt=15820
     CDS             1..146
                     /gene="LOC144105289"
                     /coded_by="XM_077638433.1:456..896"
                     /db_xref="GeneID:144105289"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrppfa altsvlccqr pasigpcvlp afgcvippdp
       61 fmlktkscme vvqatklrvi fardleqagk lvdgqwysls kasffsktkt iflgswamyl
      121 gtcgqdnagq hasasskkkh etvnky
//
LOCUS       XP_077494584             211 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105319 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077494584
VERSION     XP_077494584.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638458.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..211
                     /product="uncharacterized protein LOC144105319 isoform X1"
                     /calculated_mol_wt=23931
     Region          <68..139
                     /region_name="MULE"
                     /note="MULE transposase domain; pfam10551"
                     /db_xref="CDD:463145"
     CDS             1..211
                     /gene="LOC144105319"
                     /coded_by="XM_077638458.1:40..675"
                     /db_xref="GeneID:144105319"
ORIGIN      
        1 mlvcfagtdv sitsvaddsw avlvvtpimh raqqlesard iifvdstssc detrstitvl
       61 ltatkagavp ialvihssqt regykqafqq lksqhplcfg nspapavfmt dnsrpekdal
      121 hetwpsarql lchfhvlqae wrwltstknq aakeerrhlm rafqkvmqtl sfdylprpyn
      181 tkekrdtlhi khfampilae tlhyseirmy s
//
LOCUS       XP_077494588             183 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105319 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077494588
VERSION     XP_077494588.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638462.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="uncharacterized protein LOC144105319 isoform X2"
                     /calculated_mol_wt=21038
     Region          <40..111
                     /region_name="MULE"
                     /note="MULE transposase domain; pfam10551"
                     /db_xref="CDD:463145"
     CDS             1..183
                     /gene="LOC144105319"
                     /coded_by="XM_077638462.1:168..719"
                     /db_xref="GeneID:144105319"
ORIGIN      
        1 mhraqqlesa rdiifvdsts scdetrstit vlltatkaga vpialvihss qtregykqaf
       61 qqlksqhplc fgnspapavf mtdnsrpekd alhetwpsar qllchfhvlq aewrwltstk
      121 nqaakeerrh lmrafqkvmq tlsfdylprp yntkekrdtl hikhfampil aetlhyseir
      181 mys
//
LOCUS       XP_077494598             111 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105333 [Amblyomma americanum].
ACCESSION   XP_077494598
VERSION     XP_077494598.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638472.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..111
                     /product="uncharacterized protein LOC144105333"
                     /calculated_mol_wt=11342
     CDS             1..111
                     /gene="LOC144105333"
                     /coded_by="XM_077638472.1:26..361"
                     /db_xref="GeneID:144105333"
ORIGIN      
        1 maescsvgva dsrgpglsrs rsvhsaehlk aspkvkslss kmkspssgpw rrrslslgaq
       61 fpeaappent kwddkasear sgegaeglal gsqpvrspgr tclvsatvgs p
//
LOCUS       XP_077494617             201 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105343 [Amblyomma americanum].
ACCESSION   XP_077494617
VERSION     XP_077494617.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638491.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..201
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..201
                     /product="uncharacterized protein LOC144105343"
                     /calculated_mol_wt=22314
     CDS             1..201
                     /gene="LOC144105343"
                     /coded_by="XM_077638491.1:358..963"
                     /db_xref="GeneID:144105343"
ORIGIN      
        1 mwkvesgcvt lkvapkltrs hifpngfekm wvdlafqvfs apvrnalafy keqieskypn
       61 sdptrivigl manlieamts rfpaealrcl sfyslarspk ggnvpdgvve sllsveelln
      121 etdseeqkae srplrsdaae vsvdhasyis eqsdarliyy iagymarkrv lsidceycka
      181 aclmkkedvp ssdlpteasm q
//
LOCUS       XP_077494634             414 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105376 [Amblyomma americanum].
ACCESSION   XP_077494634
VERSION     XP_077494634.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638508.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..414
                     /product="uncharacterized protein LOC144105376"
                     /calculated_mol_wt=43790
     Region          <201..>246
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     Region          240..>397
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..414
                     /gene="LOC144105376"
                     /coded_by="XM_077638508.1:24..1268"
                     /db_xref="GeneID:144105376"
ORIGIN      
        1 msshgaaiaa vagrgnrfas dadkdyeivl ptlptgrvvl ntvflhgdvr arpyrvedfr
       61 dalanagalp dvvalgayqi nhvwavtfst adaakklaat kelevkgrrc ivfdpedhqv
      121 klrlhwmlhg vadedirtaf aafgnvtevt rerwrvagmk ekgsttrtvl lklkpgvkvd
      181 dlphqvrvag elalvvvpgr pmqclrcggt ghvrrdckvp rcskcrrygh seadcvrtya
      241 satghvaasv saelmdvvea eeaasgaddt gkaegvavqp avassntdta vaqesgspat
      301 dpsstpvetp kdgasssvhv erkaaseshd ekddsrmnvg vttpakrpha etkadgekaa
      361 epsvgeppak tpqarraglr prpkvpaenr ggdkappdqe hvlapddtgg dgiv
//
LOCUS       XP_077494672             191 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105428 [Amblyomma americanum].
ACCESSION   XP_077494672
VERSION     XP_077494672.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638546.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..191
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..191
                     /product="uncharacterized protein LOC144105428"
                     /calculated_mol_wt=21246
     Region          5..95
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..191
                     /gene="LOC144105428"
                     /coded_by="XM_077638546.1:111..686"
                     /db_xref="GeneID:144105428"
ORIGIN      
        1 mpgcccapgc rsnydggpsv rvfkfpadem qraawtkais redfvptkht vvcekhfqts
       61 dfvrfaaytd entgnvievp lqrvrlkpaa vpsvfpgcpk ylsqppssre apaekkarle
      121 aaslrkaiel svaaqeeedr rnkicsfgel csafpklkts dfwsvvttqa nvlflelsst
      181 klpqcvhqww f
//
LOCUS       XP_077494693             172 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105444 [Amblyomma americanum].
ACCESSION   XP_077494693
VERSION     XP_077494693.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638567.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..172
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..172
                     /product="uncharacterized protein LOC144105444"
                     /calculated_mol_wt=19192
     CDS             1..172
                     /gene="LOC144105444"
                     /coded_by="XM_077638567.1:4376..4894"
                     /db_xref="GeneID:144105444"
ORIGIN      
        1 medygpelph fediyqmlqd vmenkmpaal gpceqfvvrr llgtlttvva slnlkeesev
       61 lkqqlalaaa qplmttakdt etptsetgek ttmnfpsmsv rkenlknswn plrlpaeiiq
      121 epwpiinelm srnvfrsera tgqgesapls paraggsrre pqpletfnaw kh
//
LOCUS       XP_077494717             593 aa            linear   INV 25-NOV-2025
DEFINITION  organic cation transporter protein-like, partial [Amblyomma
            americanum].
ACCESSION   XP_077494717
VERSION     XP_077494717.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638591.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         <1..593
                     /product="organic cation transporter protein-like"
     Region          60..564
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..593
                     /gene="LOC144105466"
                     /coded_by="XM_077638591.1:<1..1782"
                     /db_xref="GeneID:144105466"
ORIGIN      
        1 atyhwrsfep grfsgfrpci klyqinysya lyiwailfpl aerqreqvlr samdildvlg
       61 gfglyhvltv flvvlrafpt awtnmmtpiv apdmdhwcar pegapssiin msadewkswa
      121 vpefanrfsk chlyrvfeda ngswtvdrnq tllcnrweyd tsvyqstivn ewnlvcgrsy
      181 hrsvaqsvvm agalvgvllf gemsdrlgrr ptfftcvlvi lvfgslssla pgigwynagr
      241 fvvamgvags qattvsllme imppqyrmmm nvgfgigyti pllllpllay yirdwgylql
      301 atglsalmmv pfwfilqesp rwlltkgrmk raersvvril rinrrpvpdm dqvmsdlvlf
      361 akseskkata fhfvdffrtp klrrntlvlf alwfmgsfvy ynlvlsstsa pgnpyfnyai
      421 saaseipaal lglwvarrcs rrrsqalfll laalaivplp flpkdspgwl rigsnvlvrf
      481 lalnagfikw imalevfpts arsfgfacvl tcsrfgamva pflrdlgeat dpsvpyviqa
      541 lflllasgis lllpetlnkp lpdtfheaes isrkkipkpp kqlaeykian aap
//
LOCUS       XP_077494723             295 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105474 [Amblyomma americanum].
ACCESSION   XP_077494723
VERSION     XP_077494723.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638597.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..295
                     /product="uncharacterized protein LOC144105474"
                     /calculated_mol_wt=33516
     Region          66..>162
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     CDS             1..295
                     /gene="LOC144105474"
                     /coded_by="XM_077638597.1:133..1020"
                     /db_xref="GeneID:144105474"
ORIGIN      
        1 mrslnpfcfa mqvskpccly akkssdyfli qlpspqccla ivtecsdvii sllllsgdie
       61 tnpgpaslet vvtelkklsa gqstliaevt dlknqllttd nlisdlsrri saleghyqai
      121 qslrteiegl sthttqatrq lcdledride aenqsrrnnl ifynipdpdp setwadsekl
      181 lirhcsefld itldpktidr thrlgrhepd rcrpliakfa lfktkdeila ngrkfkntny
      241 svgedfsrrv rnvrkhlvtf aksktnrfsl ryktlfigsr ryifdepsqs vkeip
//
LOCUS       XP_077494734             309 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105485 [Amblyomma americanum].
ACCESSION   XP_077494734
VERSION     XP_077494734.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638608.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..309
                     /product="uncharacterized protein LOC144105485"
                     /calculated_mol_wt=32525
     Region          <6..133
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..309
                     /gene="LOC144105485"
                     /coded_by="XM_077638608.1:346..1275"
                     /db_xref="GeneID:144105485"
ORIGIN      
        1 mekkqaqpkr rrspptsgsp khpsphrchp aatppgpgra gprhlgplfg qsprrrqasp
       61 atlghspars plgqrllstp pargpeppaa qaprqpprdv atwtyqagtd apvgyvswrp
      121 wkrqerakrl tpparlfnvf rgrtvadadq raegvslpls pgtllcpcga vmlheeahka
      181 gdlhdqrtrg attrderhpa aatpsaaarq mlaragreae faewllqvaa rhaggppeps
      241 lgsqpsaava ppssaaappa psdeardpdp krpapalaaa elpapdteag atpagglemd
      301 ldlwsteie
//
LOCUS       XP_077494742             309 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105485 [Amblyomma americanum].
ACCESSION   XP_077494742
VERSION     XP_077494742.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638616.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..309
                     /product="uncharacterized protein LOC144105485"
                     /calculated_mol_wt=32525
     Region          <6..133
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..309
                     /gene="LOC144105485"
                     /coded_by="XM_077638616.1:341..1270"
                     /db_xref="GeneID:144105485"
ORIGIN      
        1 mekkqaqpkr rrspptsgsp khpsphrchp aatppgpgra gprhlgplfg qsprrrqasp
       61 atlghspars plgqrllstp pargpeppaa qaprqpprdv atwtyqagtd apvgyvswrp
      121 wkrqerakrl tpparlfnvf rgrtvadadq raegvslpls pgtllcpcga vmlheeahka
      181 gdlhdqrtrg attrderhpa aatpsaaarq mlaragreae faewllqvaa rhaggppeps
      241 lgsqpsaava ppssaaappa psdeardpdp krpapalaaa elpapdteag atpagglemd
      301 ldlwsteie
//
LOCUS       XP_077494772             631 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105520 [Amblyomma americanum].
ACCESSION   XP_077494772
VERSION     XP_077494772.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638646.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..631
                     /product="uncharacterized protein LOC144105520"
                     /calculated_mol_wt=70119
     Region          5..91
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          277..462
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..631
                     /gene="LOC144105520"
                     /coded_by="XM_077638646.1:1..1896"
                     /db_xref="GeneID:144105520"
ORIGIN      
        1 mpghccvpgc rgnydgektv rvftfpadad rrrqwlkaip radfepgkrs vvcerhfkes
       61 dirtskyvds ktgkvveakl kiarlssdai pgvfpncpay lsapattsre aptekrmrle
      121 agslrgaiam slqtheeeea knnidtfqvl leclpqikls dfwnvisrpa cvlffnlavd
      181 dvprvlssvt vlenltvkvh yrnvqlttid gidtiphtvh dircliglld vvesfhgkll
      241 skheekidgl pklalslled alnceladve qpnavsflke qvslllvkhs rsprysaell
      301 vfssilftvs phayrflhss gnlrlphqst irricnsysv saaaeqqgts flsyakklva
      361 amtehekivv lmmdeihlqp yfdykggviv gaatnspnaa ktahvfmmqs llsrqknvvh
      421 ilpverinaq qlhtilhsii tqlehvglri iaviadnnsi nrktlslfga pcklqtttyv
      481 agycahaafk klscmtcken lmledeieve ggelvkamtr gslkfpqpai vnavltaeiv
      541 ldklrseqha tqfhalpnqk eallalthdv lndgidfdvc enghtpqlvm hyvlsaagnt
      601 flnnlckrkn dqlivekaak ekerklktlk n
//
LOCUS       XP_077494782             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105531 [Amblyomma americanum].
ACCESSION   XP_077494782
VERSION     XP_077494782.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638656.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144105531"
                     /calculated_mol_wt=16085
     CDS             1..141
                     /gene="LOC144105531"
                     /coded_by="XM_077638656.1:13..438"
                     /db_xref="GeneID:144105531"
ORIGIN      
        1 merrflkail flvcvltpfg rcqegvrdds hvenvvhkav adefsseilt nrtraylsca
       61 sgemvvkinf sepfrgityv dydktspckf ygdgrkyyel riplkgcgtk qvgrnakfcr
      121 dgkteplegl vlscrlrfry h
//
LOCUS       XP_077494807             115 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105559 [Amblyomma americanum].
ACCESSION   XP_077494807
VERSION     XP_077494807.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638681.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..115
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..115
                     /product="uncharacterized protein LOC144105559"
                     /calculated_mol_wt=12579
     CDS             1..115
                     /gene="LOC144105559"
                     /coded_by="XM_077638681.1:7..354"
                     /db_xref="GeneID:144105559"
ORIGIN      
        1 mnrmaavvpa tlrarnssea rdpthrppfa altsvlccqr pasigpcvlp afgcvippdp
       61 fmlktkshme vvqatklrvi fardleqagk lvdgqwysls kasffckysf whgia
//
LOCUS       XP_077494846             265 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105597 [Amblyomma americanum].
ACCESSION   XP_077494846
VERSION     XP_077494846.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638720.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..265
                     /product="uncharacterized protein LOC144105597"
                     /calculated_mol_wt=30109
     Region          23..>82
                     /region_name="SWIM"
                     /note="Uncharacterized protein, contains SWIM-type Zn
                     finger domain [Function unknown]; COG4715"
                     /db_xref="CDD:443750"
     Region          220..>264
                     /region_name="PDDEXK_nuclease-like"
                     /note="PDDEXK family nucleases; cl40440"
                     /db_xref="CDD:477358"
     CDS             1..265
                     /gene="LOC144105597"
                     /coded_by="XM_077638720.1:7..804"
                     /db_xref="GeneID:144105597"
ORIGIN      
        1 msvlycgfkp idaidvyfrr pelekgrrly esnhvhsvke tdgaeisakc qsqqgthvyd
       61 vqlelvpggr rilqgkcscr ygilgdykts pfvgkrppke fspfhileyf saidspftev
      121 lqqmqkseae iicaeifdda lqaavaisqr kridaliqps cktlhmlevs gifptvriap
      181 knhlqqltpe erdfykdrvv cqsvrelcaq tidqscwqrw heerklciss ttahsilrtr
      241 rgpeqalasi lqerhfssea tsygt
//
LOCUS       XP_077494858             191 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105607 [Amblyomma americanum].
ACCESSION   XP_077494858
VERSION     XP_077494858.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638732.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..191
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..191
                     /product="uncharacterized protein LOC144105607"
                     /calculated_mol_wt=21001
     CDS             1..191
                     /gene="LOC144105607"
                     /coded_by="XM_077638732.1:155..730"
                     /db_xref="GeneID:144105607"
ORIGIN      
        1 mdpayaaqqa qpmygqqpya edpygqtamg mpsnpamspm tydpgyvnyl ygmqsppspr
       61 ryviqqpqhr kglfssdnil tvlivcgtlf lilvamlviv tgvvprltrk hhsghkrrrd
      121 famehenvad ldlsnmdapq vtvadkaghs tpkhsaaakh gsnnsstyla llrnvklrtk
      181 gyrpcnasdp f
//
LOCUS       XP_077494872              72 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylinositol-3,5-bisphosphate 3-phosphatase MTMR14-like
            [Amblyomma americanum].
ACCESSION   XP_077494872
VERSION     XP_077494872.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638746.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..72
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..72
                     /product="phosphatidylinositol-3,5-bisphosphate
                     3-phosphatase MTMR14-like"
                     /calculated_mol_wt=7801
     Region          12..>47
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     CDS             1..72
                     /gene="LOC144105625"
                     /coded_by="XM_077638746.1:167..385"
                     /db_xref="GeneID:144105625"
ORIGIN      
        1 makarvarar mrfavpvily dgknicrsat lsggleiygr tiikflgcml tgrlessevd
       61 vthslcgwki pc
//
LOCUS       XP_077494900              72 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylinositol-3,5-bisphosphate 3-phosphatase MTMR14-like
            [Amblyomma americanum].
ACCESSION   XP_077494900
VERSION     XP_077494900.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638774.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..72
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..72
                     /product="phosphatidylinositol-3,5-bisphosphate
                     3-phosphatase MTMR14-like"
                     /calculated_mol_wt=7801
     Region          12..>47
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     CDS             1..72
                     /gene="LOC144105669"
                     /coded_by="XM_077638774.1:167..385"
                     /db_xref="GeneID:144105669"
ORIGIN      
        1 makarvarar mrfavpvily dgknicrsat lsggleiygr tiikflgcml tgrlessevd
       61 vthslcgwki pc
//
LOCUS       XP_077494918             247 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105683 [Amblyomma americanum].
ACCESSION   XP_077494918
VERSION     XP_077494918.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638792.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..247
                     /product="uncharacterized protein LOC144105683"
                     /calculated_mol_wt=27764
     Region          <1..36
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..247
                     /gene="LOC144105683"
                     /coded_by="XM_077638792.1:221..964"
                     /db_xref="GeneID:144105683"
ORIGIN      
        1 mpprlqrffm clmpfnisll yvlgrdlala dalsrircds deppeselen vathatagls
       61 tlvsdttakr maketsedde lrtapleagq apcellmgrr lrarlpdfad rrapevkkht
      121 qahpqrrple algehdtvrl ldkggwttka laenavaprs ymvrkkdvnq lrrnrqhllr
      181 tneefdpsle tipdctedsa sdcaslpglr hhrspwatra prvtikprlp trlksilsqg
      241 nivlwkp
//
LOCUS       XP_077494928              72 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylinositol-3,5-bisphosphate 3-phosphatase MTMR14-like
            [Amblyomma americanum].
ACCESSION   XP_077494928
VERSION     XP_077494928.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638802.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..72
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..72
                     /product="phosphatidylinositol-3,5-bisphosphate
                     3-phosphatase MTMR14-like"
                     /calculated_mol_wt=7801
     Region          12..>47
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     CDS             1..72
                     /gene="LOC144105694"
                     /coded_by="XM_077638802.1:125..343"
                     /db_xref="GeneID:144105694"
ORIGIN      
        1 makarvarar mrfavpvily dgknicrsat lsggleiygr tiikflgcml tgrlessevd
       61 vthslcgwki pc
//
LOCUS       XP_077494938             414 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105705 [Amblyomma americanum].
ACCESSION   XP_077494938
VERSION     XP_077494938.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638812.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..414
                     /product="uncharacterized protein LOC144105705"
                     /calculated_mol_wt=46107
     CDS             1..414
                     /gene="LOC144105705"
                     /coded_by="XM_077638812.1:9..1253"
                     /db_xref="GeneID:144105705"
ORIGIN      
        1 millltcvgt llaaifvgly hkpwpfvllg iwhrqaedvp glrrltayhd ppqvaaplpp
       61 tldyvheatg rvdfrsfesv tqalrvvhsa keaipveslt iivplllslv ltgwwwykrr
      121 qsssttsads ctscrsnssl htlqesdeak hrrpggptlr syrlgrrrhs vpipsvqfpa
      181 lanfgpaest grdlaladal srircdsdep peselenvat hataglstlv sdttakrmak
      241 etseddelrt apleagqapc ellmgrrlra rlpdfadrra pevkkhtqah pqrrplealg
      301 ehdtvrlldk ggwttkalae navaprsymv rkkdvnqlrr nrqhllrtne efdpsletip
      361 dctedsasdc aslpglrhhr spwatraprv tikprlptrl ksilsqgniv lwkp
//
LOCUS       XP_077494965             414 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105730 [Amblyomma americanum].
ACCESSION   XP_077494965
VERSION     XP_077494965.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638839.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..414
                     /product="uncharacterized protein LOC144105730"
                     /calculated_mol_wt=46177
     CDS             1..414
                     /gene="LOC144105730"
                     /coded_by="XM_077638839.1:9..1253"
                     /db_xref="GeneID:144105730"
ORIGIN      
        1 millltcvgt llaaifvgly hkpwpfvllg iwhrqaedvp glrrltayhd ppqvaaplpp
       61 tldyvheatg rvdfrsfesv tqalrvvhsa keaipveslt iivplllslv ltgwwwykrr
      121 qsssttsads rtscrsnssl htlqesdeak hrrpggptlr syrlgrrrhs vpissvqfpa
      181 lanfgpaest grdlaladal srircdsdep peselenvat hataglstlv sdttakrmak
      241 etseddelrt apleagqapc ellmgrrlra rlpdfadrra pevkkhtqah pqrrplealg
      301 ehdtvrlldk ggwttkalae navaprsymv rkkdvnqlrr nrqhllrtne efdpsletip
      361 dctedsasdc aslpglrhhr spwatraprv tikprlptrl ksilnqgniv lwkp
//
LOCUS       XP_077494974             414 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105739 [Amblyomma americanum].
ACCESSION   XP_077494974
VERSION     XP_077494974.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638848.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..414
                     /product="uncharacterized protein LOC144105739"
                     /calculated_mol_wt=46170
     CDS             1..414
                     /gene="LOC144105739"
                     /coded_by="XM_077638848.1:9..1253"
                     /db_xref="GeneID:144105739"
ORIGIN      
        1 millltcvgt llaaifvgly hkpwpfvllg iwhrqaedvp glrrltayhd ppqvaaplpp
       61 tldyvheatg rvdfrsfesv tqalrvvhsa keaipveslt iivplllslv ltgwwwykrr
      121 qsssttpads rtscrsnssl htlqesdeak hrrpggptlr syrlgrrrhs vpipsvqfpa
      181 lanfgpaest grdlaladal srircdsdep peselenvat hataglstlv sdttakrmak
      241 etseddelrt apleagqapc ellmgrrlra rlpdfadrra pevkkhtqah pqrrplealg
      301 ehdtvrlldk ggwttkalae navaprsymv rkkdvnqlrr nrqhllrtne efdpsletip
      361 dctedsasdc aslpglrhhr spwatraprv tikprlptrl ksilsqgniv lwkp
//
LOCUS       XP_077494982             369 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105748 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077494982
VERSION     XP_077494982.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638856.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..369
                     /product="uncharacterized protein LOC144105748 isoform X1"
                     /calculated_mol_wt=40204
     Region          <68..>203
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     CDS             1..369
                     /gene="LOC144105748"
                     /coded_by="XM_077638856.1:21..1130"
                     /db_xref="GeneID:144105748"
ORIGIN      
        1 mltslamllt lavivvgihm yartadmted tlsvggnqvn klrkpdprif dltlgppptd
       61 apaphqgslk mkdapsspet htttqpptet rspttessgg httrttskts lgnmttekpt
      121 ptrvpeqtta pdrspganet asdkqgmkdp pvpttnvsds pssndkdgtv cptvhksfcn
      181 vngaaqffyd pdegsclsat vqrahvcnrs pnqfasnedc laacvkgrwa pehrcvqkpr
      241 fvactaedar gdwwfhdgrk cvpwsfaggr cplasapvfd taavcgaqcn dsrtrrergc
      301 llpvsqlcep sqlrlpffaa psvtdkgtaf qchrvewefl lrhrcllgsn afvnrdeche
      361 tcrrrtrqy
//
LOCUS       XP_077494990             364 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105748 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077494990
VERSION     XP_077494990.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638864.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..364
                     /product="uncharacterized protein LOC144105748 isoform X2"
                     /calculated_mol_wt=39719
     Region          <68..>198
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     CDS             1..364
                     /gene="LOC144105748"
                     /coded_by="XM_077638864.1:21..1115"
                     /db_xref="GeneID:144105748"
ORIGIN      
        1 mltslamllt lavivvgihm yartadmted tlsvggnqvn klrkpdprif dltlgppptd
       61 apaphqgslk mkdapsspet htttqpptet rspttessgg httrttskts lgnmttekpt
      121 ptrvpeqtta pdrspganet asdkqgmkdp pvpttnssnd kdgtvcptvh ksfcnvngaa
      181 qffydpdegs clsatvqrah vcnrspnqfa snedclaacv kgrwapehrc vqkprfvact
      241 aedargdwwf hdgrkcvpws faggrcplas apvfdtaavc gaqcndsrtr rergcllpvs
      301 qlcepsqlrl pffaapsvtd kgtafqchrv ewefllrhrc llgsnafvnr dechetcrrr
      361 trqy
//
LOCUS       XP_077495000             369 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105766 [Amblyomma americanum].
ACCESSION   XP_077495000
VERSION     XP_077495000.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638874.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..369
                     /product="uncharacterized protein LOC144105766"
                     /calculated_mol_wt=40202
     Region          <68..>203
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     CDS             1..369
                     /gene="LOC144105766"
                     /coded_by="XM_077638874.1:13..1122"
                     /db_xref="GeneID:144105766"
ORIGIN      
        1 mltslamllt savivvgihm yartadmted tlsvggnqvn klrkpdprif dltlgppptd
       61 apaphqgslk mkdapsspet htttqpptet rspttessgg httrttskts lgnmttekpt
      121 ptrvpeqtta pdrspganet asdkqgmkdp pvpttnvsds pssndkdgtv cptvhksfcn
      181 vngaaqffyd pdegsclsat vqrahvcnrs pnqfasiedc laacvkgrwa pehrcvqkpr
      241 fvactaedar gdwwfhdgrk cvpwsfaggr cllasapvfd taavcgahcn dsrtrrergc
      301 llpvsqlcep sqlrlpffaa psvtdkgtaf qchrvewefl lrhrcllgsn afvnrdeche
      361 tcrrrtrqy
//
LOCUS       XP_077495001             177 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105782 [Amblyomma americanum].
ACCESSION   XP_077495001
VERSION     XP_077495001.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638875.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..177
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..177
                     /product="uncharacterized protein LOC144105782"
                     /calculated_mol_wt=20015
     CDS             1..177
                     /gene="LOC144105782"
                     /coded_by="XM_077638875.1:298..831"
                     /db_xref="GeneID:144105782"
ORIGIN      
        1 maketsedde lrtapleagq apcellmgrr lrarlpdfad rrapevkkht qahpqrrple
       61 algehdtvrl ldkggwttka laenavaprs ymvrkkdvnq lrrnrqhllr tneefdpsle
      121 tipdctedsa sdcaslpglr hhrspwatra prvtikprlp trlksilsqg nivlwkp
//
LOCUS       XP_077495002             369 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105798 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077495002
VERSION     XP_077495002.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638876.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..369
                     /product="uncharacterized protein LOC144105798 isoform X1"
                     /calculated_mol_wt=40189
     Region          <68..>203
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     CDS             1..369
                     /gene="LOC144105798"
                     /coded_by="XM_077638876.1:37..1146"
                     /db_xref="GeneID:144105798"
ORIGIN      
        1 mltslamllt lavivvgihm yartadmted tlsvggnqvn klrkpdprif dltlgppptd
       61 apaphqgslk mkdapsspet htttqpptet rspttessgg httrttskts lgnmttekpt
      121 ptrvpeqtta pdrspganet asdkqgmkdp pvpttnvsds pssndkdgtv cptvhksfcn
      181 vngaaqffyd pdegsclsat vqrahvcnrs pnqfasiedc laacvkgrwa pehrcvqkpr
      241 fvactaddar gdwwfhdgrk cvpwsfaggr cplasapvfd taavcgaqcn dsrtrrergc
      301 llpvsqlcep sqlrlpffaa psvtdkgtaf qchrvewefl lrhrcllgsn afvnrdeche
      361 tcrrrtrqy
//
LOCUS       XP_077495003             364 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105798 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077495003
VERSION     XP_077495003.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638877.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..364
                     /product="uncharacterized protein LOC144105798 isoform X2"
                     /calculated_mol_wt=39704
     Region          <68..>198
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     CDS             1..364
                     /gene="LOC144105798"
                     /coded_by="XM_077638877.1:37..1131"
                     /db_xref="GeneID:144105798"
ORIGIN      
        1 mltslamllt lavivvgihm yartadmted tlsvggnqvn klrkpdprif dltlgppptd
       61 apaphqgslk mkdapsspet htttqpptet rspttessgg httrttskts lgnmttekpt
      121 ptrvpeqtta pdrspganet asdkqgmkdp pvpttnssnd kdgtvcptvh ksfcnvngaa
      181 qffydpdegs clsatvqrah vcnrspnqfa siedclaacv kgrwapehrc vqkprfvact
      241 addargdwwf hdgrkcvpws faggrcplas apvfdtaavc gaqcndsrtr rergcllpvs
      301 qlcepsqlrl pffaapsvtd kgtafqchrv ewefllrhrc llgsnafvnr dechetcrrr
      361 trqy
//
LOCUS       XP_077495004             414 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105824 [Amblyomma americanum].
ACCESSION   XP_077495004
VERSION     XP_077495004.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638878.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..414
                     /product="uncharacterized protein LOC144105824"
                     /calculated_mol_wt=46054
     CDS             1..414
                     /gene="LOC144105824"
                     /coded_by="XM_077638878.1:9..1253"
                     /db_xref="GeneID:144105824"
ORIGIN      
        1 millltcvgt llaaifvgly hkpwpfvllg iwhrqaedvp glrrltayhd ppqvaaplpp
       61 tldnvheatg rvdfrsfesv tqalrvvhsa keaipveslt iivplllslv ltgwwwykrs
      121 qsssttsavs rtscrsnssl htlqesdeak hrrpggptlr syrlgrrrhs vpipsvqfpa
      181 lanfgpaest grdlaladal srircdsdep peselenvat hataglstlv sdttakrmak
      241 etseddelrt apleagqapc ellmgrrlra rlpdfadrra pevkkhtqvh pqrrplealg
      301 ehdtvrlldk ggwttkalae navaprsymv rkkdvnqlrr nrqhllrtne efdpsletip
      361 dctedsasdc aslpglrhhr spwatraprv tikprlptrl ksilsqgniv lwkp
//
LOCUS       XP_077495005             111 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105852 [Amblyomma americanum].
ACCESSION   XP_077495005
VERSION     XP_077495005.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638879.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..111
                     /product="uncharacterized protein LOC144105852"
                     /calculated_mol_wt=12580
     Region          <58..105
                     /region_name="P2RX7_C"
                     /note="P2X purinoreceptor 7 intracellular domain;
                     pfam20478"
                     /db_xref="CDD:466627"
     CDS             1..111
                     /gene="LOC144105852"
                     /coded_by="XM_077638879.1:412..747"
                     /db_xref="GeneID:144105852"
ORIGIN      
        1 mptalestcc ceipaatvkq psgcitehsh fytlcldevv ltvalqmlld hglrvehtgr
       61 yrytsysqla hwlwrrlgrh hrkvlpacav cqirerfpse gytgfqyaqg l
//
LOCUS       XP_077495006             163 aa            linear   INV 25-NOV-2025
DEFINITION  histone-lysine N-methyltransferase PRDM9-like [Amblyomma
            americanum].
ACCESSION   XP_077495006
VERSION     XP_077495006.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638880.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..163
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..163
                     /product="histone-lysine N-methyltransferase PRDM9-like"
                     /calculated_mol_wt=18476
     Region          1..104
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(1,55..60,71..73,94..97)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Site            order(1,56..60,96)
                     /site_type="other"
                     /note="SAM binding site [polypeptide binding]"
                     /db_xref="CDD:380914"
     CDS             1..163
                     /gene="LOC144105879"
                     /coded_by="XM_077638880.1:22..513"
                     /db_xref="GeneID:144105879"
ORIGIN      
        1 mgvftlkalp kglhfgpyhg vkvdcieygg ctwpvrrcae ffvvdgrqqe ryhwmnnvny
       61 spskrrqnlv avlfngniyy rtlknvgpge eplvgystsf aesllgnprg rgalkeedvn
      121 ivplhslfla cencgdlfst qhnlemhtrr khwhsthgil rct
//
LOCUS       XP_077495007             350 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105906 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077495007
VERSION     XP_077495007.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638881.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..350
                     /product="uncharacterized protein LOC144105906 isoform X1"
                     /calculated_mol_wt=38226
     Region          <49..>184
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     CDS             1..350
                     /gene="LOC144105906"
                     /coded_by="XM_077638881.1:15..1067"
                     /db_xref="GeneID:144105906"
ORIGIN      
        1 myartadmte dtlsvggnqv nklrkpdpri fdltlgpppt dapaphqgsl kmkdapsspe
       61 thtttqppte trspttessg ghttrttskt slgnmttekp tptrvpeqtt apdrspgane
      121 tasdkqgmkd ppvpttnvsd spssndkdgt vcptvhksfc nvngaaqffy dpdegsclsa
      181 tvqrahvcnr spnqfasied claacvkgrw apehrcvqkp rfvactaeda rgdwwfhdgr
      241 kcvpwsfagg rcplasapvf dtaavcgaqc ndsrtrrerg cllpvsqlce psqlrlpffa
      301 apsvtdkgta fqchrvewef llrhrcllgs nafvnrdech etcrrrtrqy
//
LOCUS       XP_077495008             345 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105906 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077495008
VERSION     XP_077495008.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638882.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..345
                     /product="uncharacterized protein LOC144105906 isoform X2"
                     /calculated_mol_wt=37740
     Region          <49..>179
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     CDS             1..345
                     /gene="LOC144105906"
                     /coded_by="XM_077638882.1:15..1052"
                     /db_xref="GeneID:144105906"
ORIGIN      
        1 myartadmte dtlsvggnqv nklrkpdpri fdltlgpppt dapaphqgsl kmkdapsspe
       61 thtttqppte trspttessg ghttrttskt slgnmttekp tptrvpeqtt apdrspgane
      121 tasdkqgmkd ppvpttnssn dkdgtvcptv hksfcnvnga aqffydpdeg sclsatvqra
      181 hvcnrspnqf asiedclaac vkgrwapehr cvqkprfvac taedargdww fhdgrkcvpw
      241 sfaggrcpla sapvfdtaav cgaqcndsrt rrergcllpv sqlcepsqlr lpffaapsvt
      301 dkgtafqchr vewefllrhr cllgsnafvn rdechetcrr rtrqy
//
LOCUS       XP_077495009             351 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105943 [Amblyomma americanum].
ACCESSION   XP_077495009
VERSION     XP_077495009.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638883.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..351
                     /product="uncharacterized protein LOC144105943"
                     /calculated_mol_wt=38459
     Region          <49..>184
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     CDS             1..351
                     /gene="LOC144105943"
                     /coded_by="XM_077638883.1:15..1070"
                     /db_xref="GeneID:144105943"
ORIGIN      
        1 myartadmte dtlsvggnqv nklrkpdpri fdltlgpppt dapaphqgsl kmkdapsspe
       61 thtttqppte trspttessg ghttrttskt slgnmttekp tptrvpeqtt apdrspgane
      121 tasdkqgmkd ppvpttnvsd spssndkdgt vcptvhkffc nvngaaqffy dpdegsclsa
      181 tvqrahvcnr spnqfasied claacvkgrw apehrcvqkp rfvactaeda rgdwwfydgr
      241 kcvpwsfagg rcplasapvf dtaavcgaqc ndsrtrrerg cllpvsqlce psqlrlpfff
      301 aapsvtdkgt afqchrvewe fllrhrcllg snafvnrdec hetcrrrtrq y
//
LOCUS       XP_077495010              72 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylinositol-3,5-bisphosphate 3-phosphatase MTMR14-like
            [Amblyomma americanum].
ACCESSION   XP_077495010
VERSION     XP_077495010.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638884.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..72
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..72
                     /product="phosphatidylinositol-3,5-bisphosphate
                     3-phosphatase MTMR14-like"
                     /calculated_mol_wt=7801
     Region          12..>47
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     CDS             1..72
                     /gene="LOC144105959"
                     /coded_by="XM_077638884.1:125..343"
                     /db_xref="GeneID:144105959"
ORIGIN      
        1 makarvarar mrfavpvily dgknicrsat lsggleiygr tiikflgcml tgrlessevd
       61 vthslcgwki pc
//
LOCUS       XP_077495011             369 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105986 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077495011
VERSION     XP_077495011.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638885.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..369
                     /product="uncharacterized protein LOC144105986 isoform X1"
                     /calculated_mol_wt=40259
     Region          <68..>203
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     CDS             1..369
                     /gene="LOC144105986"
                     /coded_by="XM_077638885.1:360..1469"
                     /db_xref="GeneID:144105986"
ORIGIN      
        1 mltslamllt lavivvgihm yartadmted tlsvggnqvn klrkpdprif dltlgppptd
       61 apaphqgslk mkdapsspet htttqpptet rsprtessgg httrttskts lgnmttekpt
      121 ptrvpeqtta pdrspganet asdkqgmkdp pvpttnvsds pssndkdgtv cptvhksfcn
      181 vngaaqffyd pdegsclsat vqrahvcnrs pnqfasiedc laacvkgrwa pehrcvqkpr
      241 fvactaedar gdwwfhdgrk cvpwsfaggr cplasapvfd taavcgakcn dsrtrrergc
      301 llpvsqlcep sqlrlpffaa psvtdkgtaf qchrvewefl lrhrcllgsn afvnrdeche
      361 tcrrrtrqy
//
LOCUS       XP_077495012             364 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144105986 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077495012
VERSION     XP_077495012.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638886.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..364
                     /product="uncharacterized protein LOC144105986 isoform X2"
                     /calculated_mol_wt=39773
     Region          <68..>198
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     CDS             1..364
                     /gene="LOC144105986"
                     /coded_by="XM_077638886.1:360..1454"
                     /db_xref="GeneID:144105986"
ORIGIN      
        1 mltslamllt lavivvgihm yartadmted tlsvggnqvn klrkpdprif dltlgppptd
       61 apaphqgslk mkdapsspet htttqpptet rsprtessgg httrttskts lgnmttekpt
      121 ptrvpeqtta pdrspganet asdkqgmkdp pvpttnssnd kdgtvcptvh ksfcnvngaa
      181 qffydpdegs clsatvqrah vcnrspnqfa siedclaacv kgrwapehrc vqkprfvact
      241 aedargdwwf hdgrkcvpws faggrcplas apvfdtaavc gakcndsrtr rergcllpvs
      301 qlcepsqlrl pffaapsvtd kgtafqchrv ewefllrhrc llgsnafvnr dechetcrrr
      361 trqy
//
LOCUS       XP_077495013             369 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106024 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077495013
VERSION     XP_077495013.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638887.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..369
                     /product="uncharacterized protein LOC144106024 isoform X1"
                     /calculated_mol_wt=40208
     Region          <68..>203
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     CDS             1..369
                     /gene="LOC144106024"
                     /coded_by="XM_077638887.1:13..1122"
                     /db_xref="GeneID:144106024"
ORIGIN      
        1 mltslamllt savivvgihm yartadmted tlsvggnqvn klrkpdprif dltlgppptd
       61 apaphqgslk mkdapsspet htttqpptet rspttessgg httrttskts lgnmttekpt
      121 ptrvpeqtta pdrspganet asdkqgmkdp pvpttnvsds pssndkdgtv cptvhksfcn
      181 vngaaqffyd pdegsclsat vqrahvcnrs pnqfasiedc laacvkgrwa pehrcvqkpr
      241 fvactaedar gdwwfhdgrk cvpwsfaggr cllasapvfd taavcgaqcn dsrtrrergc
      301 qlpvsqlcep sqlrlpffaa psvtdkgtaf qchrvewefl lrhrcllgsn afvnrdeche
      361 tcrrrtrqy
//
LOCUS       XP_077495014             364 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106024 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077495014
VERSION     XP_077495014.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638888.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..364
                     /product="uncharacterized protein LOC144106024 isoform X2"
                     /calculated_mol_wt=39723
     Region          <68..>198
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     CDS             1..364
                     /gene="LOC144106024"
                     /coded_by="XM_077638888.1:13..1107"
                     /db_xref="GeneID:144106024"
ORIGIN      
        1 mltslamllt savivvgihm yartadmted tlsvggnqvn klrkpdprif dltlgppptd
       61 apaphqgslk mkdapsspet htttqpptet rspttessgg httrttskts lgnmttekpt
      121 ptrvpeqtta pdrspganet asdkqgmkdp pvpttnssnd kdgtvcptvh ksfcnvngaa
      181 qffydpdegs clsatvqrah vcnrspnqfa siedclaacv kgrwapehrc vqkprfvact
      241 aedargdwwf hdgrkcvpws faggrcllas apvfdtaavc gaqcndsrtr rergcqlpvs
      301 qlcepsqlrl pffaapsvtd kgtafqchrv ewefllrhrc llgsnafvnr dechetcrrr
      361 trqy
//
LOCUS       XP_077495015             177 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106061 [Amblyomma americanum].
ACCESSION   XP_077495015
VERSION     XP_077495015.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638889.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..177
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..177
                     /product="uncharacterized protein LOC144106061"
                     /calculated_mol_wt=20015
     CDS             1..177
                     /gene="LOC144106061"
                     /coded_by="XM_077638889.1:298..831"
                     /db_xref="GeneID:144106061"
ORIGIN      
        1 maketsedde lrtapleagq apcellmgrr lrarlpdfad rrapevkkht qahpqrrple
       61 algehdtvrl ldkggwttka laenavaprs ymvrkkdvnq lrrnrqhllr tneefdpsle
      121 tipdctedsa sdcaslpglr hhrspwatra prvtikprlp trlksilsqg nivlwkp
//
LOCUS       XP_077495016              72 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylinositol-3,5-bisphosphate 3-phosphatase MTMR14-like
            [Amblyomma americanum].
ACCESSION   XP_077495016
VERSION     XP_077495016.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638890.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..72
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..72
                     /product="phosphatidylinositol-3,5-bisphosphate
                     3-phosphatase MTMR14-like"
                     /calculated_mol_wt=7801
     Region          12..>47
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     CDS             1..72
                     /gene="LOC144106077"
                     /coded_by="XM_077638890.1:40..258"
                     /db_xref="GeneID:144106077"
ORIGIN      
        1 makarvarar mrfavpvily dgknicrsat lsggleiygr tiikflgcml tgrlessevd
       61 vthslcgwki pc
//
LOCUS       XP_077495017             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106104 [Amblyomma americanum].
ACCESSION   XP_077495017
VERSION     XP_077495017.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638891.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144106104"
                     /calculated_mol_wt=24790
     CDS             1..217
                     /gene="LOC144106104"
                     /coded_by="XM_077638891.1:106..759"
                     /db_xref="GeneID:144106104"
ORIGIN      
        1 meskidslla mssklesles svsllsdkfd efqtrllaqe kstkdvtkrv erlekaewac
       61 eiaqlnkdvd slewrsrrln ieihglpete nedllkkvne igtkleldev cssditalhr
      121 lpakpgktrc vivrfakqev rdawlakrqa lrddntgkym cenmtrytrt lltvakewak
      181 esgyafvwhv ngkvlvrkqa garavvirdk ddlanlr
//
LOCUS       XP_077495018             142 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106120 [Amblyomma americanum].
ACCESSION   XP_077495018
VERSION     XP_077495018.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638892.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..142
                     /product="uncharacterized protein LOC144106120"
                     /calculated_mol_wt=16137
     Region          <73..>136
                     /region_name="GumC"
                     /note="Exopolysaccharide export protein/domain GumC/Wzc1
                     [Cell wall/membrane/envelope biogenesis]; COG3206"
                     /db_xref="CDD:442439"
     CDS             1..142
                     /gene="LOC144106120"
                     /coded_by="XM_077638892.1:346..774"
                     /db_xref="GeneID:144106120"
ORIGIN      
        1 mrgvtrldfn akdlgtlrvy cpniwygctq sselrhleeh ylkncphhpk mcfhcrredi
       61 ppgdfvphiy sckrrqeear svdrqsppvq qslsespasq tedehlcatq allaevterg
      121 gevhpepveh lnqlaadikr ap
//
LOCUS       XP_077495019             118 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106136 [Amblyomma americanum].
ACCESSION   XP_077495019
VERSION     XP_077495019.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638893.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..118
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..118
                     /product="uncharacterized protein LOC144106136"
                     /calculated_mol_wt=13178
     CDS             1..118
                     /gene="LOC144106136"
                     /coded_by="XM_077638893.1:9..365"
                     /db_xref="GeneID:144106136"
ORIGIN      
        1 millltcvgt llaaifvgly hkpwpfvllg iwhrqaedvp glrrltayhd ppqvaaplpp
       61 tldyvheatg rvdfrsfesv tqalrvvhsa keaipveslt iivplllslv ltgwwwyk
//
LOCUS       XP_077495020             111 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106152 [Amblyomma americanum].
ACCESSION   XP_077495020
VERSION     XP_077495020.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638894.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..111
                     /product="uncharacterized protein LOC144106152"
                     /calculated_mol_wt=12645
     Region          <5..105
                     /region_name="P2RX7_C"
                     /note="P2X purinoreceptor 7 intracellular domain;
                     pfam20478"
                     /db_xref="CDD:466627"
     CDS             1..111
                     /gene="LOC144106152"
                     /coded_by="XM_077638894.1:563..898"
                     /db_xref="GeneID:144106152"
ORIGIN      
        1 mptalestcc reipaatvkq psgcitehsh fytlcldevv ltvalqmlld hglrvehtgr
       61 yryisysqla hwlwrrlgrh hrkvlpacav cqirerfpse gytgfqyaqg l
//
LOCUS       XP_077495030             432 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106163 [Amblyomma americanum].
ACCESSION   XP_077495030
VERSION     XP_077495030.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638904.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..432
                     /product="uncharacterized protein LOC144106163"
                     /calculated_mol_wt=48020
     CDS             1..432
                     /gene="LOC144106163"
                     /coded_by="XM_077638904.1:231..1529"
                     /db_xref="GeneID:144106163"
ORIGIN      
        1 mgtyasiaaa lhvvyhmkec lppgslmiiv pvllsliltr rrrtqessrp aspmacctfv
       61 tpsrddvrwg rsrteedaai gpplvptekr eledsrraqr cslhkldrhr prrnkkeptr
      121 tgssgnhgas gcqeqrrpqs apagewlasc degkdsrlps tdalgngfhg rpghgssasq
      181 esvevarrqc ddddeekvli tdlswnsfdv tdeshpsgta dswpmgikth fpermsampa
      241 ephavaeadg eelsdrhcrd hcltavakpe vgttaetfgg reldellqlc gqkrpatlsd
      301 llqelgvdkc vklrerdgee ifrvstgrgd slltllhddy ltrhwkrtld dimahsgpsp
      361 evtadsfnnd agwddirkcy cvrdkysevl nktcinyfyl kgctkfgede rgmilpymvv
      421 lkplpgqtsn yl
//
LOCUS       XP_077495040             378 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106171 [Amblyomma americanum].
ACCESSION   XP_077495040
VERSION     XP_077495040.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638914.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..378
                     /product="uncharacterized protein LOC144106171"
                     /calculated_mol_wt=39860
     Region          30..113
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     CDS             1..378
                     /gene="LOC144106171"
                     /coded_by="XM_077638914.1:1..1137"
                     /db_xref="GeneID:144106171"
ORIGIN      
        1 mgsqqfclkw kthqsnmlaa feqlwfkeal vdvtlacegl slkahkmvls afspffqalf
       61 aenpckhpiv ilndmrymdl kaivefmyrg evnvsqddlm allktaktlk vkglaegtne
      121 nkngglvtsv dgaadagpvg tpraeslpls krkrsrrlrr sspsdsnkse skgappatri
      181 kgpdspeiid dgsmssdrvt dlpaaspssr asnvaswsst avpsaarhns svqasisqht
      241 qdsigddvge gddadfdvep ldvmeesatt envpefpdvd sssqalgdes qqshhkgstf
      301 ynsarvtvqr sllsdisats qvdikpspss lipyddqtip pgvaaspaaa lsgdgastam
      361 avmdasgapa iagpsgyq
//
LOCUS       XP_077495058             185 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger protein 711-like [Amblyomma americanum].
ACCESSION   XP_077495058
VERSION     XP_077495058.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638932.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..185
                     /product="zinc finger protein 711-like"
                     /calculated_mol_wt=21255
     Region          <14..97
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          17..37
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(17,20,33,37)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(22,24,26,28..29,32..33,36,50,52,56..57,60..61,64,78,
                     80,82,84..85,88..89,93)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          45..65
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          73..94
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          102..118
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..185
                     /gene="LOC144106191"
                     /coded_by="XM_077638932.1:2..559"
                     /db_xref="GeneID:144106191"
ORIGIN      
        1 mhtrwkhwns megihncthc sytstrkvdi irherthvdr resvchlcqk rfsrpdnlkl
       61 hmmihsgdkp yecgqcskrf rqrvharrhe qvihsrrypl tcprcgagae dvtklrrhtc
      121 kplgttegke gfkrgrgrpr lmadgtapkv rprntvegtq lqvgvkrgrg rprkviaqda
      181 sapap
//
LOCUS       XP_077495067             185 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger protein 711-like [Amblyomma americanum].
ACCESSION   XP_077495067
VERSION     XP_077495067.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638941.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..185
                     /product="zinc finger protein 711-like"
                     /calculated_mol_wt=21255
     Region          <14..97
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          17..37
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(17,20,33,37)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(22,24,26,28..29,32..33,36,50,52,56..57,60..61,64,78,
                     80,82,84..85,88..89,93)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          45..65
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          73..94
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          102..118
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..185
                     /gene="LOC144106202"
                     /coded_by="XM_077638941.1:2..559"
                     /db_xref="GeneID:144106202"
ORIGIN      
        1 mhtrwkhwns megihncthc sytstrkvdi irherthvdr resvchlcqk rfsrpdnlkl
       61 hmmihsgdkp yecgqcskrf rqrvharrhe qvihsrrypl tcprcgagae dvtklrrhtc
      121 kplgttegke gfkrgrgrpr lmadgtapkv rprntvegtq lqvgvkrgrg rprkviaqda
      181 sapap
//
LOCUS       XP_077495077             346 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106210 [Amblyomma americanum].
ACCESSION   XP_077495077
VERSION     XP_077495077.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638951.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..346
                     /product="uncharacterized protein LOC144106210"
                     /calculated_mol_wt=39131
     Region          147..168
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          <156..258
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          178..198
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(178,181,194,198)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(183,185,187,189..190,193..194,197,211,213,217..218,
                     221..222,225,239,241,243,245..246,249..250,254)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          206..226
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          234..255
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          263..279
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..346
                     /gene="LOC144106210"
                     /coded_by="XM_077638951.1:88..1128"
                     /db_xref="GeneID:144106210"
ORIGIN      
        1 matngvasrq deakavepne etqsgrppap eyilleqwea hgsggpyfpe nsasceeqrd
       61 ekpfdgreiv aradaalddv mreaieafge tlrkslpwss pdalpdhwea pgsgdpnlpe
      121 epssrevqhd enqdiniipl hslflacekc gdlftkqynm emhtrwkhwn smegihncth
      181 csytstrkvd iirherthvd rresvchlcq krfsrpdnlk lhmmihsgdk pyecgqcskr
      241 frqrvharrh eqvihsrryp ltcprcgaga edvtklrrht ckplgttegk egfkrgrgrp
      301 rlmadgtapk vrprntvegt qlqvgvkrgr grprkviaqd asapap
//
LOCUS       XP_077495088             266 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger Y-chromosomal protein-like [Amblyomma americanum].
ACCESSION   XP_077495088
VERSION     XP_077495088.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638962.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..266
                     /product="zinc finger Y-chromosomal protein-like"
                     /calculated_mol_wt=30377
     Region          <76..178
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          98..118
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(98,101,114,118)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(103,105,107,109..110,113..114,117,131,133,137..138,
                     141..142,145,159,161,163,165..166,169..170,174)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          126..146
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          138..163
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          154..175
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          183..199
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..266
                     /gene="LOC144106217"
                     /coded_by="XM_077638962.1:223..1023"
                     /db_xref="GeneID:144106217"
ORIGIN      
        1 mreaieafge tlrkslpwss pdalpdhwea pgsgdpnlpe epssrevqhd enqdiniipl
       61 hslflacekc gdlftkqynm emhtrwkhwn smegihncth csytstrkvd iirperthvd
      121 rresvchlcq krfsrpdnlk lhmmihsgdk pyecgqcskr frqrvharrh eqvihsrryp
      181 ltcprcgaga edvtklrrht ckplgttegk egfkrgrgrp rlmadgtapk vrprntvegt
      241 qlqvgvkrgr grprkviaqd asapap
//
LOCUS       XP_077495097             346 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106226 [Amblyomma americanum].
ACCESSION   XP_077495097
VERSION     XP_077495097.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638971.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..346
                     /product="uncharacterized protein LOC144106226"
                     /calculated_mol_wt=39131
     Region          147..168
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          <156..258
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          178..198
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(178,181,194,198)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(183,185,187,189..190,193..194,197,211,213,217..218,
                     221..222,225,239,241,243,245..246,249..250,254)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          206..226
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          234..255
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          263..279
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..346
                     /gene="LOC144106226"
                     /coded_by="XM_077638971.1:88..1128"
                     /db_xref="GeneID:144106226"
ORIGIN      
        1 matngvasrq deakavepne etqsgrppap eyilleqwea hgsggpyfpe nsasceeqrd
       61 ekpfdgreiv aradaalddv mreaieafge tlrkslpwss pdalpdhwea pgsgdpnlpe
      121 epssrevqhd enqdiniipl hslflacekc gdlftkqynm emhtrwkhwn smegihncth
      181 csytstrkvd iirherthvd rresvchlcq krfsrpdnlk lhmmihsgdk pyecgqcskr
      241 frqrvharrh eqvihsrryp ltcprcgaga edvtklrrht ckplgttegk egfkrgrgrp
      301 rlmadgtapk vrprntvegt qlqvgvkrgr grprkviaqd asapap
//
LOCUS       XP_077495120             354 aa            linear   INV 25-NOV-2025
DEFINITION  histone-lysine N-methyltransferase MECOM-like [Amblyomma
            americanum].
ACCESSION   XP_077495120
VERSION     XP_077495120.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077638994.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..354
                     /product="histone-lysine N-methyltransferase MECOM-like"
                     /calculated_mol_wt=39876
     Region          10..128
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(24..25,79..84,95..97,118..121)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Site            order(24..25,80..84,120)
                     /site_type="other"
                     /note="SAM binding site [polypeptide binding]"
                     /db_xref="CDD:380914"
     Region          155..178
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(155,158,171,178)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(195,197..198,201..202,205,219,221,225..226,229..230,
                     233,247,249,251,253..254,257..258)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          214..234
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          214..234
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          226..251
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          242..258
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..354
                     /gene="LOC144106234"
                     /coded_by="XM_077638994.1:1..1065"
                     /db_xref="GeneID:144106234"
ORIGIN      
        1 mpvsavracp elltlgkstv ngcpvgvftl kalpkglhfg pyqgvkvddi enggctwpvr
       61 rcgeffvvdg rpqqrnhwmn dvnyspskrr hnlvalfvng diyyrtlknv gpgeemlvry
      121 stsfaksllg nprepgalng edvnivplhs llhacekcgd lfstqykset htrrkhwhkt
      181 egirrgthcp nksshkadii rharthvdkr ksmchvckkr fskpdhlkvh mmihsgekpy
      241 ecgqcgkrfr qrvharrhee vihfrryplt cqrcgagaed vfklrhhtck plgttedkeg
      301 lkrcrgrprl radgtspkvr pqnttegtpl qggvkrgrgp prkavtqdas apra
//
LOCUS       XP_077495129             336 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106241 [Amblyomma americanum].
ACCESSION   XP_077495129
VERSION     XP_077495129.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639003.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..336
                     /product="uncharacterized protein LOC144106241"
                     /calculated_mol_wt=37862
     Region          137..160
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(137,140,153,160)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          168..188
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(168,171,184,188)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(173,175,177,179..180,183..184,187,201,203,207..208,
                     211..212,215,229,231,233,235..236,239..240,244)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          196..216
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          201..>258
                     /region_name="transpos_IS66"
                     /note="IS66 family transposase; NF033517"
                     /db_xref="CDD:468053"
     Region          208..231
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          224..245
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          253..269
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..336
                     /gene="LOC144106241"
                     /coded_by="XM_077639003.1:59..1069"
                     /db_xref="GeneID:144106241"
ORIGIN      
        1 mreaieafge thresppsts pdalpdhwea pgsggpnlpe eppsreeqhe dnpidgsgtf
       61 asvytalnea ekeasdafgd thresppsts pdalpdhwea pgsgdqhlle epssreeqhd
      121 knqdvntipl hslflacekc sdlftkqynl emhtrrkhwh stegihhcsh ctytstrkad
      181 iirherthvg rrdsvchlcq krfsrpdhlk vhmiihsgek pyecsqcgkm freraharrh
      241 eqvihsrryp ltcprcgaga edvtrlrrht ckplgttdgn eelkrgrnrp rlradgmapk
      301 vrprnttegm pqqggvkrgr grtrkviaqd esapap
//
LOCUS       XP_077495147              72 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylinositol-3,5-bisphosphate 3-phosphatase MTMR14-like
            [Amblyomma americanum].
ACCESSION   XP_077495147
VERSION     XP_077495147.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639021.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..72
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..72
                     /product="phosphatidylinositol-3,5-bisphosphate
                     3-phosphatase MTMR14-like"
                     /calculated_mol_wt=7801
     Region          12..>47
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     CDS             1..72
                     /gene="LOC144106257"
                     /coded_by="XM_077639021.1:129..347"
                     /db_xref="GeneID:144106257"
ORIGIN      
        1 makarvarar mrfavpvily dgknicrsat lsggleiygr tiikflgcml tgrlessevd
       61 vthslcgwki pc
//
LOCUS       XP_077495153             116 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106263 [Amblyomma americanum].
ACCESSION   XP_077495153
VERSION     XP_077495153.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639027.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..116
                     /product="uncharacterized protein LOC144106263"
                     /calculated_mol_wt=12080
     CDS             1..116
                     /gene="LOC144106263"
                     /coded_by="XM_077639027.1:494..844"
                     /db_xref="GeneID:144106263"
ORIGIN      
        1 madagdklvd vlthfevscs hrpvripred iramgafsdr yigrpdktgg kchhgsqepg
       61 vggavgsvqq naagrpaaag spstpasdsv delvqqlaal kplsfrrgks yrcght
//
LOCUS       XP_077495158             116 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106263 [Amblyomma americanum].
ACCESSION   XP_077495158
VERSION     XP_077495158.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639032.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..116
                     /product="uncharacterized protein LOC144106263"
                     /calculated_mol_wt=12080
     CDS             1..116
                     /gene="LOC144106263"
                     /coded_by="XM_077639032.1:149..499"
                     /db_xref="GeneID:144106263"
ORIGIN      
        1 madagdklvd vlthfevscs hrpvripred iramgafsdr yigrpdktgg kchhgsqepg
       61 vggavgsvqq naagrpaaag spstpasdsv delvqqlaal kplsfrrgks yrcght
//
LOCUS       XP_077495166             116 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106263 [Amblyomma americanum].
ACCESSION   XP_077495166
VERSION     XP_077495166.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639040.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..116
                     /product="uncharacterized protein LOC144106263"
                     /calculated_mol_wt=12080
     CDS             1..116
                     /gene="LOC144106263"
                     /coded_by="XM_077639040.1:194..544"
                     /db_xref="GeneID:144106263"
ORIGIN      
        1 madagdklvd vlthfevscs hrpvripred iramgafsdr yigrpdktgg kchhgsqepg
       61 vggavgsvqq naagrpaaag spstpasdsv delvqqlaal kplsfrrgks yrcght
//
LOCUS       XP_077495173             116 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106263 [Amblyomma americanum].
ACCESSION   XP_077495173
VERSION     XP_077495173.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639047.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..116
                     /product="uncharacterized protein LOC144106263"
                     /calculated_mol_wt=12080
     CDS             1..116
                     /gene="LOC144106263"
                     /coded_by="XM_077639047.1:183..533"
                     /db_xref="GeneID:144106263"
ORIGIN      
        1 madagdklvd vlthfevscs hrpvripred iramgafsdr yigrpdktgg kchhgsqepg
       61 vggavgsvqq naagrpaaag spstpasdsv delvqqlaal kplsfrrgks yrcght
//
LOCUS       XP_077495181             116 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106263 [Amblyomma americanum].
ACCESSION   XP_077495181
VERSION     XP_077495181.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639055.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..116
                     /product="uncharacterized protein LOC144106263"
                     /calculated_mol_wt=12080
     CDS             1..116
                     /gene="LOC144106263"
                     /coded_by="XM_077639055.1:159..509"
                     /db_xref="GeneID:144106263"
ORIGIN      
        1 madagdklvd vlthfevscs hrpvripred iramgafsdr yigrpdktgg kchhgsqepg
       61 vggavgsvqq naagrpaaag spstpasdsv delvqqlaal kplsfrrgks yrcght
//
LOCUS       XP_077495201              72 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylinositol-3,5-bisphosphate 3-phosphatase MTMR14-like
            [Amblyomma americanum].
ACCESSION   XP_077495201
VERSION     XP_077495201.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639075.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..72
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..72
                     /product="phosphatidylinositol-3,5-bisphosphate
                     3-phosphatase MTMR14-like"
                     /calculated_mol_wt=7801
     Region          12..>47
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     CDS             1..72
                     /gene="LOC144106295"
                     /coded_by="XM_077639075.1:40..258"
                     /db_xref="GeneID:144106295"
ORIGIN      
        1 makarvarar mrfavpvily dgknicrsat lsggleiygr tiikflgcml tgrlessevd
       61 vthslcgwki pc
//
LOCUS       XP_077495223              72 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylinositol-3,5-bisphosphate 3-phosphatase MTMR14-like
            [Amblyomma americanum].
ACCESSION   XP_077495223
VERSION     XP_077495223.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639097.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..72
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..72
                     /product="phosphatidylinositol-3,5-bisphosphate
                     3-phosphatase MTMR14-like"
                     /calculated_mol_wt=7801
     Region          12..>47
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     CDS             1..72
                     /gene="LOC144106319"
                     /coded_by="XM_077639097.1:40..258"
                     /db_xref="GeneID:144106319"
ORIGIN      
        1 makarvarar mrfavpvily dgknicrsat lsggleiygr tiikflgcml tgrlessevd
       61 vthslcgwki pc
//
LOCUS       XP_077495232             177 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106328 [Amblyomma americanum].
ACCESSION   XP_077495232
VERSION     XP_077495232.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639106.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..177
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..177
                     /product="uncharacterized protein LOC144106328"
                     /calculated_mol_wt=20015
     CDS             1..177
                     /gene="LOC144106328"
                     /coded_by="XM_077639106.1:515..1048"
                     /db_xref="GeneID:144106328"
ORIGIN      
        1 maketsedde lrtapleagq apcellmgrr lrarlpdfad rrapevkkht qahpqrrple
       61 algehdtvrl ldkggwttka laenavaprs ymvrkkdvnq lrrnrqhllr tneefdpsle
      121 tipdctedsa sdcaslpglr hhrspwatra prvtikprlp trlksilsqg nivlwkp
//
LOCUS       XP_077495255             111 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106350 [Amblyomma americanum].
ACCESSION   XP_077495255
VERSION     XP_077495255.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639129.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..111
                     /product="uncharacterized protein LOC144106350"
                     /calculated_mol_wt=12645
     Region          <5..105
                     /region_name="P2RX7_C"
                     /note="P2X purinoreceptor 7 intracellular domain;
                     pfam20478"
                     /db_xref="CDD:466627"
     CDS             1..111
                     /gene="LOC144106350"
                     /coded_by="XM_077639129.1:266..601"
                     /db_xref="GeneID:144106350"
ORIGIN      
        1 mptalestcc reipaatvkq psgcitehsh fytlcldevv ltvalqmlld hglrvehtgr
       61 yryisysqla hwlwrrlgrh hrkvlpacav cqirerfpse gytgfqyaqg l
//
LOCUS       XP_077495266             378 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106357 [Amblyomma americanum].
ACCESSION   XP_077495266
VERSION     XP_077495266.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639140.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..378
                     /product="uncharacterized protein LOC144106357"
                     /calculated_mol_wt=39864
     Region          30..113
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     CDS             1..378
                     /gene="LOC144106357"
                     /coded_by="XM_077639140.1:1..1137"
                     /db_xref="GeneID:144106357"
ORIGIN      
        1 mgsqqfclkw kthqsnmlaa feqlwfkeal vdvtlacegl slkahkmvls afspffqalf
       61 aenpckhpiv ilndmrymdl kaivefmyrg evnvsqddlm allktaetlk vkglaegtne
      121 nkngglvtsv dgaadagpvg tpraeslpls krkrsrrlrr sspsdsnkse skgappatri
      181 kgpdspeiid dgsmssdrvt dlpaaspssr asnvaswsst avpstarhns svqasisqht
      241 qdsigddvge gddadfdvep ldvmeesatt envpefpdvd sssqalgdes qqshhtgstf
      301 ynsarvtvqr sllsdisats qvdikpspss lipyddqtip pgvaaspaaa lsgdgastam
      361 avmdasgapa iagpsgyq
//
LOCUS       XP_077495287             266 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger Y-chromosomal protein-like [Amblyomma americanum].
ACCESSION   XP_077495287
VERSION     XP_077495287.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639161.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..266
                     /product="zinc finger Y-chromosomal protein-like"
                     /calculated_mol_wt=30357
     Region          <76..178
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          98..118
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(98,101,114,118)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(103,105,107,109..110,113..114,117,131,133,137..138,
                     141..142,145,159,161,163,165..166,169..170,174)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          126..146
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          154..175
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          183..199
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..266
                     /gene="LOC144106378"
                     /coded_by="XM_077639161.1:223..1023"
                     /db_xref="GeneID:144106378"
ORIGIN      
        1 mreaieafge tlrkslpwss pdalpdhwea pgsgdpnlpe epssrevqhd enqdiniipl
       61 hslflacekc gdlftkqynm emhtrwkhwn smegihncth csytstrkvd iirherthvd
      121 rresvchlcq krfsrpdnlk lhmmihsgdk pyecgqcskr frqrvharrh eqvihsrryp
      181 ltcprcgaga edvtklrrht ckplgttegk egskrgrgrp rlmadgtapk vrprntvegt
      241 qlqvgvkrgr grprkviaqd asapap
//
LOCUS       XP_077495302             163 aa            linear   INV 25-NOV-2025
DEFINITION  histone-lysine N-methyltransferase PRDM9-like [Amblyomma
            americanum].
ACCESSION   XP_077495302
VERSION     XP_077495302.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639176.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..163
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..163
                     /product="histone-lysine N-methyltransferase PRDM9-like"
                     /calculated_mol_wt=18506
     Region          1..104
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(1,55..60,71..73,94..97)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Site            order(1,56..60,96)
                     /site_type="other"
                     /note="SAM binding site [polypeptide binding]"
                     /db_xref="CDD:380914"
     CDS             1..163
                     /gene="LOC144106382"
                     /coded_by="XM_077639176.1:8..499"
                     /db_xref="GeneID:144106382"
ORIGIN      
        1 mgvftlkalp kglhfgpyhg vkvdcieygg ctwpvrrcae ffvvdgrqqe ryhwmnnvny
       61 spskrrqnlv avlfngniyy rtlknvgpge ellvgystsf aesllgkprg rgalkeedvn
      121 ivplhslfla cencgdlfst qhnlemhtrr khwhsthgil rct
//
LOCUS       XP_077495314             155 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106389 [Amblyomma americanum].
ACCESSION   XP_077495314
VERSION     XP_077495314.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639188.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..155
                     /product="uncharacterized protein LOC144106389"
                     /calculated_mol_wt=16876
     CDS             1..155
                     /gene="LOC144106389"
                     /coded_by="XM_077639188.1:21..488"
                     /db_xref="GeneID:144106389"
ORIGIN      
        1 mppkpfavvk fpmeddslav vpvgwlshdw qhchwpkrra aevirlaree adpsapgcew
       61 frcpvavvtt crsyknaekk akkyemssav essaisgneg pdgpdvrlps ppprlpsppp
      121 rlpsppprlp spppqlpspp rlisppckpl svfsl
//
LOCUS       XP_077495325             142 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106397 [Amblyomma americanum].
ACCESSION   XP_077495325
VERSION     XP_077495325.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639199.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..142
                     /product="uncharacterized protein LOC144106397"
                     /calculated_mol_wt=16137
     Region          <73..>136
                     /region_name="GumC"
                     /note="Exopolysaccharide export protein/domain GumC/Wzc1
                     [Cell wall/membrane/envelope biogenesis]; COG3206"
                     /db_xref="CDD:442439"
     CDS             1..142
                     /gene="LOC144106397"
                     /coded_by="XM_077639199.1:346..774"
                     /db_xref="GeneID:144106397"
ORIGIN      
        1 mrgvtrldfn akdlgtlrvy cpniwygctq sselrhleeh ylkncphhpk mcfhcrredi
       61 ppgdfvphiy sckrrqeear svdrqsppvq qslsespasq tedehlcatq allaevterg
      121 gevhpepveh lnqlaadikr ap
//
LOCUS       XP_077495337             177 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106404 [Amblyomma americanum].
ACCESSION   XP_077495337
VERSION     XP_077495337.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639211.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..177
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..177
                     /product="uncharacterized protein LOC144106404"
                     /calculated_mol_wt=20015
     CDS             1..177
                     /gene="LOC144106404"
                     /coded_by="XM_077639211.1:298..831"
                     /db_xref="GeneID:144106404"
ORIGIN      
        1 maketsedde lrtapleagq apcellmgrr lrarlpdfad rrapevkkht qahpqrrple
       61 algehdtvrl ldkggwttka laenavaprs ymvrkkdvnq lrrnrqhllr tneefdpsle
      121 tipdctedsa sdcaslpglr hhrspwatra prvtikprlp trlksilsqg nivlwkp
//
LOCUS       XP_077495356             177 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106418 [Amblyomma americanum].
ACCESSION   XP_077495356
VERSION     XP_077495356.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639230.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..177
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..177
                     /product="uncharacterized protein LOC144106418"
                     /calculated_mol_wt=20015
     CDS             1..177
                     /gene="LOC144106418"
                     /coded_by="XM_077639230.1:50..583"
                     /db_xref="GeneID:144106418"
ORIGIN      
        1 maketsedde lrtapleagq apcellmgrr lrarlpdfad rrapevkkht qahpqrrple
       61 algehdtvrl ldkggwttka laenavaprs ymvrkkdvnq lrrnrqhllr tneefdpsle
      121 tipdctedsa sdcaslpglr hhrspwatra prvtikprlp trlksilsqg nivlwkp
//
LOCUS       XP_077495371             177 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106432 [Amblyomma americanum].
ACCESSION   XP_077495371
VERSION     XP_077495371.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639245.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..177
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..177
                     /product="uncharacterized protein LOC144106432"
                     /calculated_mol_wt=20015
     CDS             1..177
                     /gene="LOC144106432"
                     /coded_by="XM_077639245.1:50..583"
                     /db_xref="GeneID:144106432"
ORIGIN      
        1 maketsedde lrtapleagq apcellmgrr lrarlpdfad rrapevkkht qahpqrrple
       61 algehdtvrl ldkggwttka laenavaprs ymvrkkdvnq lrrnrqhllr tneefdpsle
      121 tipdctedsa sdcaslpglr hhrspwatra prvtikprlp trlksilsqg nivlwkp
//
LOCUS       XP_077495397             414 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106452 [Amblyomma americanum].
ACCESSION   XP_077495397
VERSION     XP_077495397.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639271.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..414
                     /product="uncharacterized protein LOC144106452"
                     /calculated_mol_wt=46160
     CDS             1..414
                     /gene="LOC144106452"
                     /coded_by="XM_077639271.1:9..1253"
                     /db_xref="GeneID:144106452"
ORIGIN      
        1 millltcvgt llaaifvgly hkpwpfvllg iwhrqaedvp glrrltayhd ppqvaaplpp
       61 tldyvheatg rvdfrsfesv tqalrvvhsa keaipveslt iivplllslv ltgwwwykrr
      121 qsssttsads rtscrsnssl htlqesdeak hrrpggptlr syrlgrrrhs vpipsvqfpa
      181 lanfgpaest grdlaladal srircdsdep peselenvat hataglstlv sdttakrmak
      241 etseddelrt apleagqapc ellmgrrlra rlpdfadrra pevkkhtqah pqrrplealg
      301 ehdtvrlldk ggwttkalae navaprsymv rkkdvnqlrr nrqhllrtne efdpsletip
      361 dctedsasdc aslpglrhhr spwatraprv tikprlptrl ksilsqgniv lwkp
//
LOCUS       XP_077495402             142 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106459 [Amblyomma americanum].
ACCESSION   XP_077495402
VERSION     XP_077495402.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639276.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..142
                     /product="uncharacterized protein LOC144106459"
                     /calculated_mol_wt=16137
     Region          <73..>136
                     /region_name="GumC"
                     /note="Exopolysaccharide export protein/domain GumC/Wzc1
                     [Cell wall/membrane/envelope biogenesis]; COG3206"
                     /db_xref="CDD:442439"
     CDS             1..142
                     /gene="LOC144106459"
                     /coded_by="XM_077639276.1:346..774"
                     /db_xref="GeneID:144106459"
ORIGIN      
        1 mrgvtrldfn akdlgtlrvy cpniwygctq sselrhleeh ylkncphhpk mcfhcrredi
       61 ppgdfvphiy sckrrqeear svdrqsppvq qslsespasq tedehlcatq allaevterg
      121 gevhpepveh lnqlaadikr ap
//
LOCUS       XP_077495411              72 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylinositol-3,5-bisphosphate 3-phosphatase MTMR14-like
            [Amblyomma americanum].
ACCESSION   XP_077495411
VERSION     XP_077495411.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639285.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..72
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..72
                     /product="phosphatidylinositol-3,5-bisphosphate
                     3-phosphatase MTMR14-like"
                     /calculated_mol_wt=7801
     Region          12..>47
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     CDS             1..72
                     /gene="LOC144106465"
                     /coded_by="XM_077639285.1:149..367"
                     /db_xref="GeneID:144106465"
ORIGIN      
        1 makarvarar mrfavpvily dgknicrsat lsggleiygr tiikflgcml tgrlessevd
       61 vthslcgwki pc
//
LOCUS       XP_077495421             247 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106475 [Amblyomma americanum].
ACCESSION   XP_077495421
VERSION     XP_077495421.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639295.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..247
                     /product="uncharacterized protein LOC144106475"
                     /calculated_mol_wt=27800
     Region          <1..36
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..247
                     /gene="LOC144106475"
                     /coded_by="XM_077639295.1:224..967"
                     /db_xref="GeneID:144106475"
ORIGIN      
        1 mpprlqrffm clmpfnisll yvlgrdlala dalsrircds deppeselen vathatagls
       61 tlvrdttakr maketsedde lrtapleagq apcellmgrr lrarlpdfad rrapevkkht
      121 qahpqrrple algehdtvrl ldkggwttka laenavaprs ymvrkkdvnq lrrnrqhllr
      181 tneefdpsle tipdctedsa sdcaslpglr hhrspwatra prvtinprlp thlksilsqg
      241 nivlwkp
//
LOCUS       XP_077495440             369 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106490 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077495440
VERSION     XP_077495440.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639314.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..369
                     /product="uncharacterized protein LOC144106490 isoform X1"
                     /calculated_mol_wt=40189
     Region          <68..>203
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     CDS             1..369
                     /gene="LOC144106490"
                     /coded_by="XM_077639314.1:354..1463"
                     /db_xref="GeneID:144106490"
ORIGIN      
        1 mltslamllt lavivvgihm yartadmted tlsvggnqvn klrkpdprif dltlgpppsd
       61 apaphqgslk mkdapsspet htttqpptet rspttessgg httrttskts lgnmttekpt
      121 ptrvpeqtta pdrspganet asdkqgmkdp pvpttnvsds pssndkdgtv cptvhksfcn
      181 vngaaqffyd pdegsclsat vqrahvcnrs pnqfasiedc laacvkgrwa pehrcvqkpr
      241 fvactaedar gdwwfhdgrk cvpwsfaggr cplasapvfd taavcgaqcn dsrtrrergc
      301 llpvsqlcep sqlrlpffaa psvtdkgtaf qchrvewefl lrhrcllgsn afvnrdeche
      361 tcrrrtrqy
//
LOCUS       XP_077495449             364 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106490 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077495449
VERSION     XP_077495449.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639323.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..364
                     /product="uncharacterized protein LOC144106490 isoform X2"
                     /calculated_mol_wt=39704
     Region          <68..>198
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     CDS             1..364
                     /gene="LOC144106490"
                     /coded_by="XM_077639323.1:354..1448"
                     /db_xref="GeneID:144106490"
ORIGIN      
        1 mltslamllt lavivvgihm yartadmted tlsvggnqvn klrkpdprif dltlgpppsd
       61 apaphqgslk mkdapsspet htttqpptet rspttessgg httrttskts lgnmttekpt
      121 ptrvpeqtta pdrspganet asdkqgmkdp pvpttnssnd kdgtvcptvh ksfcnvngaa
      181 qffydpdegs clsatvqrah vcnrspnqfa siedclaacv kgrwapehrc vqkprfvact
      241 aedargdwwf hdgrkcvpws faggrcplas apvfdtaavc gaqcndsrtr rergcllpvs
      301 qlcepsqlrl pffaapsvtd kgtafqchrv ewefllrhrc llgsnafvnr dechetcrrr
      361 trqy
//
LOCUS       XP_077495464             177 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106498 [Amblyomma americanum].
ACCESSION   XP_077495464
VERSION     XP_077495464.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639338.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..177
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..177
                     /product="uncharacterized protein LOC144106498"
                     /calculated_mol_wt=20015
     CDS             1..177
                     /gene="LOC144106498"
                     /coded_by="XM_077639338.1:362..895"
                     /db_xref="GeneID:144106498"
ORIGIN      
        1 maketsedde lrtapleagq apcellmgrr lrarlpdfad rrapevkkht qahpqrrple
       61 algehdtvrl ldkggwttka laenavaprs ymvrkkdvnq lrrnrqhllr tneefdpsle
      121 tipdctedsa sdcaslpglr hhrspwatra prvtikprlp trlksilsqg nivlwkp
//
LOCUS       XP_077495481             350 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106510 [Amblyomma americanum].
ACCESSION   XP_077495481
VERSION     XP_077495481.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639355.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..350
                     /product="uncharacterized protein LOC144106510"
                     /calculated_mol_wt=38212
     Region          <49..>184
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     CDS             1..350
                     /gene="LOC144106510"
                     /coded_by="XM_077639355.1:36..1088"
                     /db_xref="GeneID:144106510"
ORIGIN      
        1 myartadmte dtlsvggnqv nklrkpdpri fdltlgpppt dapaphqgsl kmkdppsspe
       61 thtttqppte trspttessg ghttrttskt slgnmttekp tptrvpeqtt apdrspgane
      121 tasdkqgmkd pavpttnvsd spssndkdgt vcptvhksfc nvngaaqffy dpdegsclsa
      181 tvqrahvcnr spnqfasied claacvngrw apehrcvqkp rfvactaeda rgdwwfhdgr
      241 kcvpwsfagg rcplasapvf dtaavcgaqc ndsrtrrerg cllpvsqlce psqlrlpffa
      301 apsvtdkgta fqchrvewef llrhrcllgs nafvnrdech etcrrrtrqy
//
LOCUS       XP_077495498             142 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106524 [Amblyomma americanum].
ACCESSION   XP_077495498
VERSION     XP_077495498.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639372.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..142
                     /product="uncharacterized protein LOC144106524"
                     /calculated_mol_wt=16137
     Region          <73..>136
                     /region_name="GumC"
                     /note="Exopolysaccharide export protein/domain GumC/Wzc1
                     [Cell wall/membrane/envelope biogenesis]; COG3206"
                     /db_xref="CDD:442439"
     CDS             1..142
                     /gene="LOC144106524"
                     /coded_by="XM_077639372.1:349..777"
                     /db_xref="GeneID:144106524"
ORIGIN      
        1 mrgvtrldfn akdlgtlrvy cpniwygctq sselrhleeh ylkncphhpk mcfhcrredi
       61 ppgdfvphiy sckrrqeear svdrqsppvq qslsespasq tedehlcatq allaevterg
      121 gevhpepveh lnqlaadikr ap
//
LOCUS       XP_077495509             299 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106533 [Amblyomma americanum].
ACCESSION   XP_077495509
VERSION     XP_077495509.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639383.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..299
                     /product="uncharacterized protein LOC144106533"
                     /calculated_mol_wt=32667
     Region          <6..>133
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     CDS             1..299
                     /gene="LOC144106533"
                     /coded_by="XM_077639383.1:80..979"
                     /db_xref="GeneID:144106533"
ORIGIN      
        1 mkdapsspet htttqpptet rspttessgg httrttskts lgnmttekpt ptrvpeqtta
       61 pdrspganet asdkqgmkdp pvpttnvsds pssndkdgtv cptvhksfcn vngaaqffyd
      121 pdegsclsat vqrahvcnrs pnqfasiegc laacvkgrwa pehrcvqkpr fvactaedar
      181 gdwwfhdgrk cvpwsfaggr cplasapvfd taavcgaqcn dsrtrrergc llpvsqlcep
      241 sqlrlpffaa psvtdkgtaf qchrvewefl lrhrcllgsn afvnrdeche tcrrrtrqy
//
LOCUS       XP_077495524             369 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106548 [Amblyomma americanum].
ACCESSION   XP_077495524
VERSION     XP_077495524.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639398.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..369
                     /product="uncharacterized protein LOC144106548"
                     /calculated_mol_wt=40160
     Region          <68..>203
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     CDS             1..369
                     /gene="LOC144106548"
                     /coded_by="XM_077639398.1:20..1129"
                     /db_xref="GeneID:144106548"
ORIGIN      
        1 mltslamllt lavivvgihm yartadmted tlsvggnqvn klrkpdprif dltlgppptd
       61 apaphqgslk mkdapsspet htttqpptet rspttessgg httrttskts lgnmttekpt
      121 ptrvpeqtta pdrspganet asdkqgmkdp lvpttnvsds pssndkdgtv cptvhksfcn
      181 vngaaqffyd pdegsclsat vqrahvcnrs pnqfasiedc laacvkgrwa pehrcvqkpp
      241 fvactaedar gdwwfhdgrk cvpwsfaggr cplasapvfd taavcgaqcn dsrtrrergc
      301 llpvsqlcep sqlrlpffaa psvtdkgtaf qchrvewefl lrhrcllgsn afvnrdeche
      361 tcrrrtrqy
//
LOCUS       XP_077495551             104 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106576 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077495551
VERSION     XP_077495551.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639425.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..104
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..104
                     /product="uncharacterized protein LOC144106576 isoform X1"
                     /calculated_mol_wt=12342
     Region          43..>94
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          45..66
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Site            order(50,56,63,65,69,87)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     CDS             1..104
                     /gene="LOC144106576"
                     /coded_by="XM_077639425.1:177..491"
                     /db_xref="GeneID:144106576"
ORIGIN      
        1 meakrkaane sddsnvppee qrhmsdqrrr llnirpvged tihwcwlcdr rfhlpsrliv
       61 hqiyqhdfdg eprpikvpcp icyrlcriet iiglhlrthi gerr
//
LOCUS       XP_077495556             103 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106576 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077495556
VERSION     XP_077495556.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639430.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..103
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..103
                     /product="uncharacterized protein LOC144106576 isoform X2"
                     /calculated_mol_wt=12214
     Region          42..>93
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          44..65
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Site            order(49,55,62,64,68,86)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     CDS             1..103
                     /gene="LOC144106576"
                     /coded_by="XM_077639430.1:177..488"
                     /db_xref="GeneID:144106576"
ORIGIN      
        1 meakrkaane sddsnvppee rhmsdqrrrl lnirpvgedt ihwcwlcdrr fhlpsrlivh
       61 qiyqhdfdge prpikvpcpi cyrlcrieti iglhlrthig err
//
LOCUS       XP_077495579             100 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106602 [Amblyomma americanum].
ACCESSION   XP_077495579
VERSION     XP_077495579.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639453.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..100
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..100
                     /product="uncharacterized protein LOC144106602"
                     /calculated_mol_wt=10666
     CDS             1..100
                     /gene="LOC144106602"
                     /coded_by="XM_077639453.1:383..685"
                     /db_xref="GeneID:144106602"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrppfa altsvlccqr pasigpcvlp afgcvippdp
       61 fmlkikscme veqshqvacd fcqgpgagwe isgwpvvftq
//
LOCUS       XP_077495620             108 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106654 [Amblyomma americanum].
ACCESSION   XP_077495620
VERSION     XP_077495620.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639494.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..108
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..108
                     /product="uncharacterized protein LOC144106654"
                     /calculated_mol_wt=11300
     Region          47..103
                     /region_name="SVWC"
                     /note="Single domain von Willebrand factor type C;
                     pfam15430"
                     /db_xref="CDD:464713"
     CDS             1..108
                     /gene="LOC144106654"
                     /coded_by="XM_077639494.1:117..443"
                     /db_xref="GeneID:144106654"
ORIGIN      
        1 mnfkfartll lvavicglin lgqvatsvfv mqvpvskgkc lspygaleqd qsivpsgdcv
       61 qvtcdadsei lqiercgssn lglaenvrgk karvggvafp accwdpli
//
LOCUS       XP_077495631             174 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106664 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077495631
VERSION     XP_077495631.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639505.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..174
                     /product="uncharacterized protein LOC144106664 isoform X1"
                     /calculated_mol_wt=19598
     CDS             1..174
                     /gene="LOC144106664"
                     /coded_by="XM_077639505.1:58..582"
                     /db_xref="GeneID:144106664"
ORIGIN      
        1 mepaaragfv rvflalsftt lvlagghynh hrssgyvrvl gggqqqqqqq iklppitrtw
       61 hmaavsdcsl drcqqiceeh ygankldvtg rcdgdgcnce yrepcrqmpc yenclrtkrg
      121 klnlkancir hecfckwdnh cersscealc lrdhkgkegv rglcvgedcs crwn
//
LOCUS       XP_077495638             174 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106664 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077495638
VERSION     XP_077495638.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639512.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..174
                     /product="uncharacterized protein LOC144106664 isoform X1"
                     /calculated_mol_wt=19598
     CDS             1..174
                     /gene="LOC144106664"
                     /coded_by="XM_077639512.1:58..582"
                     /db_xref="GeneID:144106664"
ORIGIN      
        1 mepaaragfv rvflalsftt lvlagghynh hrssgyvrvl gggqqqqqqq iklppitrtw
       61 hmaavsdcsl drcqqiceeh ygankldvtg rcdgdgcnce yrepcrqmpc yenclrtkrg
      121 klnlkancir hecfckwdnh cersscealc lrdhkgkegv rglcvgedcs crwn
//
LOCUS       XP_077495645             168 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106664 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077495645
VERSION     XP_077495645.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639519.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..168
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..168
                     /product="uncharacterized protein LOC144106664 isoform X2"
                     /calculated_mol_wt=18911
     CDS             1..168
                     /gene="LOC144106664"
                     /coded_by="XM_077639519.1:57..563"
                     /db_xref="GeneID:144106664"
ORIGIN      
        1 mepaaragfv rvflalsftt lvlagghynh hrssgggqqq qqqqiklppi trtwhmaavs
       61 dcsldrcqqi ceehygankl dvtgrcdgdg cnceyrepcr qmpcyenclr tkrgklnlka
      121 ncirhecfck wdnhcerssc ealclrdhkg kegvrglcvg edcscrwn
//
LOCUS       XP_077495661             302 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106693 [Amblyomma americanum].
ACCESSION   XP_077495661
VERSION     XP_077495661.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639535.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..302
                     /product="uncharacterized protein LOC144106693"
                     /calculated_mol_wt=32682
     Region          171..>293
                     /region_name="ZnMc"
                     /note="Zinc-dependent metalloprotease. This super-family
                     of metalloproteases contains two major branches, the
                     astacin-like proteases and the adamalysin/reprolysin-like
                     proteases. Both branches have wide phylogenetic
                     distribution, and contain sub-families, which...; cl00064"
                     /db_xref="CDD:469599"
     CDS             1..302
                     /gene="LOC144106693"
                     /coded_by="XM_077639535.1:178..1086"
                     /db_xref="GeneID:144106693"
ORIGIN      
        1 mrsivgyvfi laaiaessag gqiayprvle ergtdgtvvv qvtedvtlnl ekaaltlprl
       61 rldievngtv vhkvvngskl detlfvdrkq kssvmmrlse kgvsltgivn delriqpsvp
      121 qgrsllltge hsldkidkaq vedlpgnvtp thllettlva gkggrlkhai vypevyviis
      181 snfakgvpdd alfsyiiifl agvntkladl anpkvqlrlv gvlksqaweq slqrkgsyil
      241 aaatlnqflr rvitlgvgnp diylvvteed midfmngryd sritgispig gmciagekca
      301 hc
//
LOCUS       XP_077495690             302 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106718 [Amblyomma americanum].
ACCESSION   XP_077495690
VERSION     XP_077495690.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639564.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..302
                     /product="uncharacterized protein LOC144106718"
                     /calculated_mol_wt=32435
     Region          54..>149
                     /region_name="Nucleoporin_FG2"
                     /note="Nucleoporin FG repeated region; pfam15967"
                     /db_xref="CDD:435043"
     Region          229..>268
                     /region_name="ZnF_GATA"
                     /note="zinc finger binding to DNA consensus sequence
                     [AT]GATA[AG]; smart00401"
                     /db_xref="CDD:214648"
     CDS             1..302
                     /gene="LOC144106718"
                     /coded_by="XM_077639564.1:204..1112"
                     /db_xref="GeneID:144106718"
ORIGIN      
        1 maslypgval lrtadaesep pfdasgetar lmkihdlaee mvareaaghv gdltattavg
       61 gglrgsgapa araksspdes flmtlaessl mpladaadss vrasvhktsa tesptsreas
      121 sgnsallgsq aaatsagdsa eqssstaisq qssgrrrkpt phkspgaedp nfkgavvhmr
      181 llmvrgqpql rmdhyfnnmq rrgilrpdyt elsdteddfq fvrrdyvehp gkqcascatk
      241 ltplwrdaed gtplcnacgi rykkykvrct gcwhiprkne ntrsdclecg gtlrfvvrkp
      301 ft
//
LOCUS       XP_077495714             335 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106742 [Amblyomma americanum].
ACCESSION   XP_077495714
VERSION     XP_077495714.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639588.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..335
                     /product="uncharacterized protein LOC144106742"
                     /calculated_mol_wt=37159
     CDS             1..335
                     /gene="LOC144106742"
                     /coded_by="XM_077639588.1:144..1151"
                     /db_xref="GeneID:144106742"
ORIGIN      
        1 meeapreaat mpqlaerser nadasfepda draaiengna agmsqsnapt lahdsrnedf
       61 vvmdldsalc hqhcedlpep glaerelptt daatqedgdv ekfanalkdr drrieelerl
      121 igmqqaeiar iehemvacrr ecaeqissmr qylesktsev elsapqnnka tgikpsgslh
      181 anqsderiqs dgseiasfvr ekaslrgavp vsaslldtfd ceedagvqir qevaayrhec
      241 vnqmlslrkr leascfesrl qcvahvtale rrlmemmsae retmrtslke mtllrdymts
      301 mtdvietfvs lyepasestp sathsrnvsk sdpgk
//
LOCUS       XP_077495732             100 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106757 [Amblyomma americanum].
ACCESSION   XP_077495732
VERSION     XP_077495732.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639606.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..100
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..100
                     /product="uncharacterized protein LOC144106757"
                     /calculated_mol_wt=11177
     CDS             1..100
                     /gene="LOC144106757"
                     /coded_by="XM_077639606.1:14..316"
                     /db_xref="GeneID:144106757"
ORIGIN      
        1 mdmdflpvnv vfviaavnsa ppqtfqsdqa hqhqlpeyqn pwksearrli amlhgtyhra
       61 paiaatfekk krnlqaaria aarsveerka avrtwspktv
//
LOCUS       XP_077495781             107 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106769 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077495781
VERSION     XP_077495781.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639655.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..107
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..107
                     /product="uncharacterized protein LOC144106769 isoform X1"
                     /calculated_mol_wt=12257
     Region          <1..>50
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..107
                     /gene="LOC144106769"
                     /coded_by="XM_077639655.1:339..662"
                     /db_xref="GeneID:144106769"
ORIGIN      
        1 miicdanmki lavdprcpgs chdafswrys rlrrkvehgl lddgefllgn ntlhckfwsc
       61 krqwistrav aahtsvrasw ckhcrravqr rstlpqcdvl wnaalvf
//
LOCUS       XP_077495792              72 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106769 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077495792
VERSION     XP_077495792.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639666.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..72
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..72
                     /product="uncharacterized protein LOC144106769 isoform X2"
                     /calculated_mol_wt=8187
     Region          <1..>47
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..72
                     /gene="LOC144106769"
                     /coded_by="XM_077639666.1:339..557"
                     /db_xref="GeneID:144106769"
ORIGIN      
        1 miicdanmki lavdprcpgs chdafswrys rlrrkvehgl lddgefllgk qpidhratst
       61 ariklqqwli pk
//
LOCUS       XP_077495833             129 aa            linear   INV 25-NOV-2025
DEFINITION  THAP domain-containing protein 2-like [Amblyomma americanum].
ACCESSION   XP_077495833
VERSION     XP_077495833.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639707.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..129
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..129
                     /product="THAP domain-containing protein 2-like"
                     /calculated_mol_wt=14122
     Region          4..80
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..129
                     /gene="LOC144106772"
                     /coded_by="XM_077639707.1:7..396"
                     /db_xref="GeneID:144106772"
ORIGIN      
        1 mpsccvpgcr srtpgqepgv tfhtfpkdpe rrrqwteavr pgqrdwepak wtcvcskhfl
       61 pehfdrmspl vvriradavp lqqeplsqqp gdgtavaata qhgaqqaltq shtspasplq
      121 qifsnsavl
//
LOCUS       XP_077495863             167 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106791 [Amblyomma americanum].
ACCESSION   XP_077495863
VERSION     XP_077495863.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639737.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..167
                     /product="uncharacterized protein LOC144106791"
                     /calculated_mol_wt=18208
     CDS             1..167
                     /gene="LOC144106791"
                     /coded_by="XM_077639737.1:317..820"
                     /db_xref="GeneID:144106791"
ORIGIN      
        1 mepqlqplve welkterfaa ppspsmylnt eqqripkpvy swfgvkaqqq aliiqpvfvg
       61 aysiaivhwq pgkaagshgt aaaasggmga enrtlrratf afdvpeygaa edsetcvfvv
      121 rsqgpaagsh hpaslcwcil hchrslaare gswvtwnrsc slwwngs
//
LOCUS       XP_077495873             167 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106800 [Amblyomma americanum].
ACCESSION   XP_077495873
VERSION     XP_077495873.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639747.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..167
                     /product="uncharacterized protein LOC144106800"
                     /calculated_mol_wt=18208
     CDS             1..167
                     /gene="LOC144106800"
                     /coded_by="XM_077639747.1:34..537"
                     /db_xref="GeneID:144106800"
ORIGIN      
        1 mepqlqplve welkterfaa ppspsmylnt eqqripkpvy swfgvkaqqq aliiqpvfvg
       61 aysiaivhwq pgkaagshgt aaaasggmga enrtlrratf afdvpeygaa edsetcvfvv
      121 rsqgpaagsh hpaslcwcil hchrslaare gswvtwnrsc slwwngs
//
LOCUS       XP_077495881             167 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106809 [Amblyomma americanum].
ACCESSION   XP_077495881
VERSION     XP_077495881.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639755.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..167
                     /product="uncharacterized protein LOC144106809"
                     /calculated_mol_wt=18208
     CDS             1..167
                     /gene="LOC144106809"
                     /coded_by="XM_077639755.1:496..999"
                     /db_xref="GeneID:144106809"
ORIGIN      
        1 mepqlqplve welkterfaa ppspsmylnt eqqripkpvy swfgvkaqqq aliiqpvfvg
       61 aysiaivhwq pgkaagshgt aaaasggmga enrtlrratf afdvpeygaa edsetcvfvv
      121 rsqgpaagsh hpaslcwcil hchrslaare gswvtwnrsc slwwngs
//
LOCUS       XP_077495893             167 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106818 [Amblyomma americanum].
ACCESSION   XP_077495893
VERSION     XP_077495893.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639767.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..167
                     /product="uncharacterized protein LOC144106818"
                     /calculated_mol_wt=18208
     CDS             1..167
                     /gene="LOC144106818"
                     /coded_by="XM_077639767.1:567..1070"
                     /db_xref="GeneID:144106818"
ORIGIN      
        1 mepqlqplve welkterfaa ppspsmylnt eqqripkpvy swfgvkaqqq aliiqpvfvg
       61 aysiaivhwq pgkaagshgt aaaasggmga enrtlrratf afdvpeygaa edsetcvfvv
      121 rsqgpaagsh hpaslcwcil hchrslaare gswvtwnrsc slwwngs
//
LOCUS       XP_077495901             564 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106825 [Amblyomma americanum].
ACCESSION   XP_077495901
VERSION     XP_077495901.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639775.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..564
                     /product="uncharacterized protein LOC144106825"
                     /calculated_mol_wt=62931
     Region          209..289
                     /region_name="FH_FOX"
                     /note="Forkhead (FH) domain found in Forkhead box (FOX)
                     family of transcription factors and similar proteins;
                     cl00061"
                     /db_xref="CDD:469596"
     Site            order(213,231..232,235,258..259,261..262,265,271,285..287,
                     289)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410781"
     CDS             1..564
                     /gene="LOC144106825"
                     /coded_by="XM_077639775.1:67..1761"
                     /db_xref="GeneID:144106825"
ORIGIN      
        1 mddsdwtwdg dgfqptkrpk legssstrks krprrkgsgg drsrakprer snhplkknee
       61 cgakrgrrgr drvptkekkk lgssavsqpp pgttmecghv pnteeeppav ynedlledla
      121 reyletikpd lstvvrlkgr dssltshsrr srsleyvdtt krrrassepa krsgrtrtms
      181 spagtctvak lppgplaell pagtivrrpp gklvqlvaqa ihesplqvlr vrhvyealkk
      241 rypyfmfmdk kdettwkssv rhalsqrwfk kvhthppdrn aargksnfwe inyhnrprgw
      301 ampeeeemss tgfgespept psvwsqdldm eldllpplqs pppqqqactp yqvllspqta
      361 mqrqqppvar pmpthssapt lqsssaepsl msplymlhms ppawctgmde ipvgselgpl
      421 caqekhqeqe avmpqeqslp rlgpsnsqeg tlphpelplp pqfpwppqep plpggptspp
      481 rqeetgpwgt gpgfrprsgr lqlpgwpyvg epvfaaianp rravmmedgt wyilvypafa
      541 endeclarav whweegtpad aval
//
LOCUS       XP_077495910             206 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106836 [Amblyomma americanum].
ACCESSION   XP_077495910
VERSION     XP_077495910.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639784.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..206
                     /product="uncharacterized protein LOC144106836"
                     /calculated_mol_wt=22444
     CDS             1..206
                     /gene="LOC144106836"
                     /coded_by="XM_077639784.1:86..706"
                     /db_xref="GeneID:144106836"
ORIGIN      
        1 mghargggna ehwlrgvpra yavrvvaapg hgawpaatla vssaptadvq vllspqtamq
       61 rqqppvvwpm ttlspaptlq sssaepslms plymlhmspp awctgmdeip vgselgplca
      121 qemhpeqeav mpqeqffrdw dhqirkrepc rirsclgprs srghrkgphc qedpfrshdk
      181 krpghgvrdl dsgrdlggyn crgght
//
LOCUS       XP_077495927             223 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106851 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077495927
VERSION     XP_077495927.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639801.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..223
                     /product="uncharacterized protein LOC144106851 isoform X1"
                     /calculated_mol_wt=23919
     CDS             1..223
                     /gene="LOC144106851"
                     /coded_by="XM_077639801.1:83..754"
                     /db_xref="GeneID:144106851"
ORIGIN      
        1 mhtsqkhrht tqgicsctyg tctsshrgts ggrlssragr vvqlvgpvsa ggvgrpycqq
       61 laapcqqqer aspphplrpp rghrasvgsi pgtsglqrea hsaagtqqpt alptlpssps
      121 ppsppvpgrp vlrrsqqdee vadllretrr tvdlaearnr rdaefqarll ehlneaaanr
      181 rqltatvadl aaavrdtreq svrlheqlvv avsflvlllh yqg
//
LOCUS       XP_077495936             206 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106851 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077495936
VERSION     XP_077495936.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639810.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..206
                     /product="uncharacterized protein LOC144106851 isoform X2"
                     /calculated_mol_wt=22190
     CDS             1..206
                     /gene="LOC144106851"
                     /coded_by="XM_077639810.1:108..728"
                     /db_xref="GeneID:144106851"
ORIGIN      
        1 mkideddlwr gtsggrlssr agrvvqlvgp vsaggvgrpy cqqlaapcqq qeraspphpl
       61 rpprghrasv gsipgtsglq reahsaagtq qptalptlps spsppsppvp grpvlrrsqq
      121 deevadllre trrtvdlaea rnrrdaefqa rllehlneaa anrrqltatv adlaaavrdt
      181 reqsvrlheq lvvavsflvl llhyqg
//
LOCUS       XP_077495941             194 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106851 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077495941
VERSION     XP_077495941.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639815.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..194
                     /product="uncharacterized protein LOC144106851 isoform X3"
                     /calculated_mol_wt=21479
     CDS             1..194
                     /gene="LOC144106851"
                     /coded_by="XM_077639815.1:46..630"
                     /db_xref="GeneID:144106851"
ORIGIN      
        1 mrrpllytiq lrnayqpetp tydagnlqlh vrhlheqsqe raspphplrp prghrasvgs
       61 ipgtsglqre ahsaagtqqp talptlpssp sppsppvpgr pvlrrsqqde evadllretr
      121 rtvdlaearn rrdaefqarl lehlneaaan rrqltatvad laaavrdtre qsvrlheqlv
      181 vavsflvlll hyqg
//
LOCUS       XP_077495946             275 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106873 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077495946
VERSION     XP_077495946.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639820.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..275
                     /product="uncharacterized protein LOC144106873 isoform X1"
                     /calculated_mol_wt=30855
     CDS             1..275
                     /gene="LOC144106873"
                     /coded_by="XM_077639820.1:29..856"
                     /db_xref="GeneID:144106873"
ORIGIN      
        1 mlgpftldnl ryvysikrrh yhdelkdkta rtksgeleyt grwkffdcls flcvrsepfr
       61 vavvspmlhf eeeqlvsgrd lrggcflkhv evlvylrall ffcdrdvrri eleeesdgtl
      121 lddgdtsvve aeessilpds ilptadalrv aakdeppatp stsleyalst fpppfkkvra
      181 sssavaapas slavqpliqt psdscsstvv prvenvqsls cpvggyydec dqfgnivany
      241 mrclseedrl efhchimsct kdfkcfshfg vkrrl
//
LOCUS       XP_077495955             241 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106873 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077495955
VERSION     XP_077495955.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639829.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..241
                     /product="uncharacterized protein LOC144106873 isoform X2"
                     /calculated_mol_wt=26863
     CDS             1..241
                     /gene="LOC144106873"
                     /coded_by="XM_077639829.1:29..754"
                     /db_xref="GeneID:144106873"
ORIGIN      
        1 mlgpftldnl ryvysikrrh yhdelkdkta rtksgeleyt grwkffdcls flcvrsepfr
       61 vavvspmlhf eeeqlielee esdgtllddg dtsvveaees silpdsilpt adalrvaakd
      121 eppatpstsl eyalstfppp fkkvrasssa vaapasslav qpliqtpsds csstvvprve
      181 nvqslscpvg gyydecdqfg nivanymrcl seedrlefhc himsctkdfk cfshfgvkrr
      241 l
//
LOCUS       XP_077495962             196 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106887 [Amblyomma americanum].
ACCESSION   XP_077495962
VERSION     XP_077495962.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639836.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..196
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..196
                     /product="uncharacterized protein LOC144106887"
                     /calculated_mol_wt=21904
     Region          <5..38
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..196
                     /gene="LOC144106887"
                     /coded_by="XM_077639836.1:1..591"
                     /db_xref="GeneID:144106887"
ORIGIN      
        1 maatriqrwa lhlaayryrl qyapgrqmln tdalsripqr speadddalq rpkenddwlp
       61 llgsevyaln ygagskwtpc hvkktsgarm vtvetsdgvn qrhvdqvrpr peapgdnpav
      121 ttsnthcrep dkatqlqrpe tvspdegmta rrypngtgsp dlpepdipva plntgvtqqt
      181 lrrstrerkp vqrfhf
//
LOCUS       XP_077495974             191 aa            linear   INV 25-NOV-2025
DEFINITION  regulator of G-protein signaling 2-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077495974
VERSION     XP_077495974.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639848.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..191
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..191
                     /product="regulator of G-protein signaling 2-like isoform
                     X1"
                     /calculated_mol_wt=21723
     Region          74..184
                     /region_name="RGS"
                     /note="Regulator of G protein signaling (RGS) domain
                     superfamily; cl02565"
                     /db_xref="CDD:470619"
     CDS             1..191
                     /gene="LOC144106896"
                     /coded_by="XM_077639848.1:9..584"
                     /db_xref="GeneID:144106896"
ORIGIN      
        1 mpevtsgsav ppsqrqsskk mssscitsep qqeklhkncl iellpanllk rrqtpsvrpt
       61 peeakewars frhltsskyg lalfhvflar eyseeniefw laceelktcr psklsvkaqr
      121 ifdefiaphs pkevnldalt raevsaslak rpdrsclela qrrvqgller daylrflqce
      181 lylelcrppa p
//
LOCUS       XP_077495981             171 aa            linear   INV 25-NOV-2025
DEFINITION  regulator of G-protein signaling 2-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077495981
VERSION     XP_077495981.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639855.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..171
                     /product="regulator of G-protein signaling 2-like isoform
                     X2"
                     /calculated_mol_wt=19640
     Region          54..164
                     /region_name="RGS"
                     /note="Regulator of G protein signaling (RGS) domain
                     superfamily; cl02565"
                     /db_xref="CDD:470619"
     CDS             1..171
                     /gene="LOC144106896"
                     /coded_by="XM_077639855.1:160..675"
                     /db_xref="GeneID:144106896"
ORIGIN      
        1 mssscitsep qqeklhkncl iellpanllk rrqtpsvrpt peeakewars frhltsskyg
       61 lalfhvflar eyseeniefw laceelktcr psklsvkaqr ifdefiaphs pkevnldalt
      121 raevsaslak rpdrsclela qrrvqgller daylrflqce lylelcrppa p
//
LOCUS       XP_077495993             364 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106905 [Amblyomma americanum].
ACCESSION   XP_077495993
VERSION     XP_077495993.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639867.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..364
                     /product="uncharacterized protein LOC144106905"
                     /calculated_mol_wt=40486
     Region          242..299
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cd00590"
                     /db_xref="CDD:409669"
     CDS             1..364
                     /gene="LOC144106905"
                     /coded_by="XM_077639867.1:97..1191"
                     /db_xref="GeneID:144106905"
ORIGIN      
        1 mpleksgver gpgpsrtdsw qcmedevert svsqaneqds pvvladtpmk rrkgsvvtid
       61 vacgeseeeg eitdddylei idvaddlsse eegkakgcek ttgkraakkh rgkrrgrarn
      121 fnelrledyv pecsieslek rdncslaitm kynarsvdlt elsgdivklv frgradegst
      181 mttgrpsyrh hgttkafary lspaiaaanm krlqkhigtg kvlgvekcvt rktttvrnml
      241 difrlpcdcs vrrlkehfpr aqvliikngf arlefdsved vrralkrpgc tfidghpirf
      301 slvkvlpged gagvrdqyrs gggdsgpsss grrgwhgsra swhqgwrged shgsryfwnr
      361 cggy
//
LOCUS       XP_077496029             294 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106942 [Amblyomma americanum].
ACCESSION   XP_077496029
VERSION     XP_077496029.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639903.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..294
                     /product="uncharacterized protein LOC144106942"
                     /calculated_mol_wt=32216
     CDS             1..294
                     /gene="LOC144106942"
                     /coded_by="XM_077639903.1:393..1277"
                     /db_xref="GeneID:144106942"
ORIGIN      
        1 mpsnaplata ltpaptvvma qprhpgtfcg tddvdvedwl amyervskhn awdatlmlan
       61 vifylagtar vwfetheeel tswdtckqkl rdlfgkpigr klaakkdlas raqsstesyi
      121 syiqdvlalc rkvdsamset dkvghilkgi vddafnlllc kdcakvdsvl qvcrqfeqak
      181 hrriaprfdr lpntaatssc edlptlqqps hpanltrivq releamtpia ftpqppdati
      241 avieavvrqe fanvglncgl psaspctpts tpliaaaslt paippryryr npad
//
LOCUS       XP_077496042             275 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106950 [Amblyomma americanum].
ACCESSION   XP_077496042
VERSION     XP_077496042.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639916.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..275
                     /product="uncharacterized protein LOC144106950"
                     /calculated_mol_wt=31018
     Region          80..240
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..275
                     /gene="LOC144106950"
                     /coded_by="XM_077639916.1:1..828"
                     /db_xref="GeneID:144106950"
ORIGIN      
        1 mpfedqillt lmrlrlglll ndlslrfgas taaisrifcf ivtklakfar eflvfwlprt
       61 airrtlpyvf eenysvtciv dcfeifidrp gqlhrrntty ssykshntvk ilhviapsgf
      121 imflskpygg rasdrfitld sgflnhltqg devladrgfn iddllppsvk vslpsfskgr
      181 pqicrsdvvs srrlarlrih versirrlkc frilkqfpas iftkkpfand vlvaiaglcn
      241 lqpnlirdhg dasepgaqsp shpgkqlevv dntpe
//
LOCUS       XP_077496062             934 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106967 [Amblyomma americanum].
ACCESSION   XP_077496062
VERSION     XP_077496062.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639936.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..934
                     /product="uncharacterized protein LOC144106967"
                     /calculated_mol_wt=105177
     Region          5..94
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          277..462
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..934
                     /gene="LOC144106967"
                     /coded_by="XM_077639936.1:182..2986"
                     /db_xref="GeneID:144106967"
ORIGIN      
        1 mpghccvpgc rgnydgektv rvftfpadad rrrhwlkaip radfepgkrr vvcerhfkes
       61 dirtskyvds ktgkvveakl kiaclssdai psvfpncpay lsapattsre aptekrmrle
      121 aaslrdaiam slqtheeeea knkmdtfqvl leclpqiklf dfwnvisrpa cvfflnlavd
      181 dvprvlssvt vlenftvkvh yrnvqlttid gkdtiphtvh diriliglld avesfhrkll
      241 skheekidgl lklalslled alnceladve qpnavsflke qvslllvkhs rssrysaell
      301 vfssilftvs lhayrflhss gnlrlphqst irricnsysv spaaeqqgts flsyakklva
      361 amrehekiva lmmdeihlhp yfdykrgviv gaatnsrnaa ktahvfmmqs llsrqknvvh
      421 ilpverinaq qlhtilhsii tqlehfglri iaviadnnsi nrktmslfga pcklqsvyph
      481 padskrplff lidpvhlikc vrnnwinqrn agtcmffpsp tgpnikppil tasfktlrel
      541 hskeqgqlik saptlssksl npsnierqnv klalrifsps taaalracgp rleldhvfgt
      601 aqfletitkw wkivnvktcn kgtrlrdelq spitsasspq iqfllsivew ldlwqslkfd
      661 tailtreths alrlttdtlv kvtrycldem nfeyvlsgkf qtdcleerfg kyrqlsgaqy
      721 hisirqvyes erklrlqnil elpefeaard avavndavle vfdievtgdd yakapnlpat
      781 tyvagycaha afkklscmtc nenlmledei eveggelvka mtrgslkfpq pavvnavlta
      841 eivldklrse qhttqfhalp nqkeallalt hdvlndgidf dvcenghtpq lvmhyvlsaa
      901 antllnnlck rknnqlivek aakekerklk tlkn
//
LOCUS       XP_077496097             163 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144106992 [Amblyomma americanum].
ACCESSION   XP_077496097
VERSION     XP_077496097.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639971.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..163
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..163
                     /product="uncharacterized protein LOC144106992"
                     /calculated_mol_wt=18171
     Region          81..161
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..163
                     /gene="LOC144106992"
                     /coded_by="XM_077639971.1:309..800"
                     /db_xref="GeneID:144106992"
ORIGIN      
        1 maaeivvldg vqtlaypelg pdgmpvfeeg lpvyvtdggv rlrlaireqe aagavgsdsa
       61 esreqeaaav ssdsaqtdel wpkqktllli qlykefkesp grrlktkkqm wemlaakite
      121 vfggnltpsq ienkwrtler gykrvrehns rsgndrrsce fee
//
LOCUS       XP_077496103             115 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107002 [Amblyomma americanum].
ACCESSION   XP_077496103
VERSION     XP_077496103.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077639977.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..115
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..115
                     /product="uncharacterized protein LOC144107002"
                     /calculated_mol_wt=12762
     CDS             1..115
                     /gene="LOC144107002"
                     /coded_by="XM_077639977.1:209..556"
                     /db_xref="GeneID:144107002"
ORIGIN      
        1 mkeecvgsdp aaqrsrhsgr mleimeevag svhaggkdag ngdaerqrlr ftdnmgirsq
       61 wiftktaavr eklhptskgr mewrmmrtll erqssvpcin vhrhllgalm atvsc
//
LOCUS       XP_077496144             498 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107033 [Amblyomma americanum].
ACCESSION   XP_077496144
VERSION     XP_077496144.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640018.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..498
                     /product="uncharacterized protein LOC144107033"
                     /calculated_mol_wt=55760
     Region          66..>205
                     /region_name="SWIM"
                     /note="Uncharacterized protein, contains SWIM-type Zn
                     finger domain [Function unknown]; COG4715"
                     /db_xref="CDD:443750"
     Region          299..489
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(355,394,408,410)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..498
                     /gene="LOC144107033"
                     /coded_by="XM_077640018.1:11..1507"
                     /db_xref="GeneID:144107033"
ORIGIN      
        1 mlsasdyvvs lsgadqkqym eklhvygaqr pdpytvpese wvqgaegkrr cppvkmsnvv
       61 vyllfspskf tagnvenyka leaynyfesg lvrkcrlwrp gdvqisfvra evmpsqtaaa
      121 kghdtwvcvn dtsgqilagh cqckaglgev cshvaallfv leaaarerad vsctsqpcaw
      181 haavskkatm vpvdqlnlsk pgrkarklaa eanhtsetyd ardieifqrl rkasssavvl
      241 rsipktdsed tdsasedeem ytflrnarka rstkedfled mflplseier iekatrltsk
      301 splwhearyg rltgttmhri rtlkadtnvq glldsvlqrr pplnieavrw gadkekvake
      361 qylefmkach anfelheigf vidtdapflg atpdglwhcs chgvglleik cpwslkephh
      421 qvleasylsa sgtllethty ftqvqtqlhv crkavchfvv wlphqfgive vpysshffkp
      481 llekaravwa ehvlpall
//
LOCUS       XP_077496153             138 aa            linear   INV 25-NOV-2025
DEFINITION  BEN domain-containing protein 5-like [Amblyomma americanum].
ACCESSION   XP_077496153
VERSION     XP_077496153.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640027.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..138
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..138
                     /product="BEN domain-containing protein 5-like"
                     /calculated_mol_wt=14146
     CDS             1..138
                     /gene="LOC144107040"
                     /coded_by="XM_077640027.1:13..429"
                     /db_xref="GeneID:144107040"
ORIGIN      
        1 masygisaas dlmvnpamvv gtpadaqqlg raapkkpaar vsktlgvpvv gsgfgtpged
       61 gqvqlgngvh vnadvwvalm mqttdaafck rlavalwgve vlakrsvfgk qsnkdiakgk
      121 tmvhpplspm klvgmsce
//
LOCUS       XP_077496185             198 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107071 [Amblyomma americanum].
ACCESSION   XP_077496185
VERSION     XP_077496185.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640059.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..198
                     /product="uncharacterized protein LOC144107071"
                     /calculated_mol_wt=22455
     Region          <1..>180
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..198
                     /gene="LOC144107071"
                     /coded_by="XM_077640059.1:21..617"
                     /db_xref="GeneID:144107071"
ORIGIN      
        1 mllldptlsg crivfresmr kfhsdhsdly vlktskprvl ylnrpmitil eqsgikaevf
       61 lmlqnkilds fidsmmdphe aahvlrayca lrlpykelag vgidltvepf fralvravnk
      121 kvlkelrtka rilvppnygr tmfgvldetg tleygqvfvq ysndmlrykv ndpatilegi
      181 pansffaskh saawsrci
//
LOCUS       XP_077496226             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107110 [Amblyomma americanum].
ACCESSION   XP_077496226
VERSION     XP_077496226.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640100.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144107110"
                     /calculated_mol_wt=24744
     CDS             1..217
                     /gene="LOC144107110"
                     /coded_by="XM_077640100.1:82..735"
                     /db_xref="GeneID:144107110"
ORIGIN      
        1 meskidslla mssklesles svsllsdkfd efqtrllaqe kstkdvtkrv erlekaewac
       61 eiaqlnkdvd slewrsrrln ieihglpete nedllkkvne igtkleldev cssditalhr
      121 lpakpgktrg vivrfakqev rdawlakrqa lrddntgkym cenmtrytrt lltvakewak
      181 esgyafvwhv ngkvlvrkqa garavvirdk ddlanlr
//
LOCUS       XP_077496237             462 aa            linear   INV 25-NOV-2025
DEFINITION  chitinase-3-like protein 2 [Amblyomma americanum].
ACCESSION   XP_077496237
VERSION     XP_077496237.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640111.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..462
                     /product="chitinase-3-like protein 2"
                     /calculated_mol_wt=51675
     Region          104..409
                     /region_name="Glyco_hydro_18"
                     /note="Glycosyl hydrolases family 18; pfam00704"
                     /db_xref="CDD:425828"
     CDS             1..462
                     /gene="LOC144107121"
                     /coded_by="XM_077640111.1:67..1455"
                     /db_xref="GeneID:144107121"
ORIGIN      
        1 msrymedeqw llhqsqprsl esrkdpcvkt cityfgaacv sftlvlvgvf vfmiaydvrk
       61 adstaaaifp vkvrtqkhrp apilchinva rfydsellyk ttelpgnycs hlvfplrgfl
      121 sggnetpdsp ltfglvqsrl ielrdkvnns fphlkylvaa gdengggrla fhdtpnatay
      181 mtflvemvrw liqtrfdglv lnsvfpmene hrrhmavfls hlkqamhkhg llfvvtarwd
      241 tgifksgarp grlskfldyv gvvthslhra anatdrilvp fhhqrgqkns rsvedsidhv
      301 issgvprskv litvslagyv ctvtqlpekd evptwssara lrvvsygeac hlvrqpdwal
      361 sydhesekph awrnnlwmgy edeisvrkta slvdkmylgg vliwdvgndd htgrcgltnp
      421 lvraifteve gtpiddnpga iipsfnitda dahtgnktkl sg
//
LOCUS       XP_077496258             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107143 [Amblyomma americanum].
ACCESSION   XP_077496258
VERSION     XP_077496258.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640132.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144107143"
                     /calculated_mol_wt=14985
     CDS             1..133
                     /gene="LOC144107143"
                     /coded_by="XM_077640132.1:348..749"
                     /db_xref="GeneID:144107143"
ORIGIN      
        1 mhlwkllfnt yialqvnmem nggnepldpt sqeavlhtlk llrqmspdev daclechlcs
       61 ddhrarpave rltrtsafgr eralverqav csswpstvqa sftgwlpyan wlqpfngnrt
      121 wmknllgtls kvg
//
LOCUS       XP_077496273             199 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107147 [Amblyomma americanum].
ACCESSION   XP_077496273
VERSION     XP_077496273.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640147.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..199
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..199
                     /product="uncharacterized protein LOC144107147"
                     /calculated_mol_wt=20564
     CDS             1..199
                     /gene="LOC144107147"
                     /coded_by="XM_077640147.1:180..779"
                     /db_xref="GeneID:144107147"
ORIGIN      
        1 mpranlashr slsspsassp rghaapaple lkplatttat gakakrsfsy ekglrqstpc
       61 raqqstvppp tcevfafvan allpallalp pastsstavy csddrifawp qpdvitsscp
      121 aapppgyhfp gpswkasspa tsatgyissd rppsplcgla gcasvhttnp fvyimqgrlq
      181 aasavdvvny dpklttisd
//
LOCUS       XP_077496297             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-1 chain-like [Amblyomma americanum].
ACCESSION   XP_077496297
VERSION     XP_077496297.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640171.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-1 chain-like"
                     /calculated_mol_wt=49541
     Region          1..430
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144107171"
                     /coded_by="XM_077640171.1:52..1383"
                     /db_xref="GeneID:144107171"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi drsgayhgns dlqlerinvy yneasggkyv
       61 prailvdlep gavdavrsgp ygplfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrgcqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkdssy fvewipnnvk tavcdipprg
      361 lkmsatfign staiqevfkr iseqftamfr rkaflhwytg egmdefefte adsnlnelvs
      421 eyqqyqeatp edeeefeetq aea
//
LOCUS       XP_077496309             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077496309
VERSION     XP_077496309.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640183.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like isoform X1"
                     /calculated_mol_wt=49511
     Region          1..430
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144107178"
                     /coded_by="XM_077640183.1:199..1530"
                     /db_xref="GeneID:144107178"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gavdavrsgp ygplfrpdnf vfgqsgagnn wakghytega elvdsaldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrgcqq yrvltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkdssy fvewipnnvk tavcdipprg
      361 lkmsatfign staiqelfkr iseqftamfr rkaflhwytg egmdefefte aesnlnelvs
      421 eyqqyqeatp edeeefeetq aea
//
LOCUS       XP_077496318             442 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077496318
VERSION     XP_077496318.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640192.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..442
                     /product="tubulin beta-4 chain-like isoform X2"
                     /calculated_mol_wt=49454
     Region          1..429
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..442
                     /gene="LOC144107178"
                     /coded_by="XM_077640192.1:198..1526"
                     /db_xref="GeneID:144107178"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasgkyvp
       61 railvdlepg avdavrsgpy gplfrpdnfv fgqsgagnnw akghytegae lvdsaldvvr
      121 keaescdclq gfqlthslgg gtgsgmgtll iskireeypd rimntysvvp spkvsdtvve
      181 pynatlsvhq lventdetyc idnealydic frtlklttpt ygdlnhlvsa tmsgvttclr
      241 fpgqlnadlr klavnmvpfp rlhffmtgfa pltsrgcqqy rvltvpeltq qmfdaknmma
      301 acdprhgryl tvaavfrgrm smrevddqml niqnkdssyf vewipnnvkt avcdipprgl
      361 kmsatfigns taiqelfkri seqftamfrr kaflhwytge gmdefeftea esnlnelvse
      421 yqqyqeatpe deeefeetqa ea
//
LOCUS       XP_077496328             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077496328
VERSION     XP_077496328.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640202.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like isoform X1"
                     /calculated_mol_wt=49543
     Region          1..430
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144107188"
                     /coded_by="XM_077640202.1:89..1420"
                     /db_xref="GeneID:144107188"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gavdavrsgp ygplfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrgcqq yraltvpelt qqmfdaknmm
      301 aaceprhgry ltvaavfrgr msmrevddqm lniqnkdssy fvewipnnvk tavcdipprg
      361 lkmsatfign staiqelfkr iseqftamfr rkafmhwytg egmdefefte aesnlnelvs
      421 eyqqyqeatp edeeefeetq aea
//
LOCUS       XP_077496333             442 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077496333
VERSION     XP_077496333.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640207.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..442
                     /product="tubulin beta-4 chain-like isoform X2"
                     /calculated_mol_wt=49486
     Region          1..429
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..442
                     /gene="LOC144107188"
                     /coded_by="XM_077640207.1:89..1417"
                     /db_xref="GeneID:144107188"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasgkyvp
       61 railvdlepg avdavrsgpy gplfrpdnfv fgqsgagnnw akghytegae lvdsvldvvr
      121 keaescdclq gfqlthslgg gtgsgmgtll iskireeypd rimntysvvp spkvsdtvve
      181 pynatlsvhq lventdetyc idnealydic frtlklttpt ygdlnhlvsa tmsgvttclr
      241 fpgqlnadlr klavnmvpfp rlhffmtgfa pltsrgcqqy raltvpeltq qmfdaknmma
      301 aceprhgryl tvaavfrgrm smrevddqml niqnkdssyf vewipnnvkt avcdipprgl
      361 kmsatfigns taiqelfkri seqftamfrr kafmhwytge gmdefeftea esnlnelvse
      421 yqqyqeatpe deeefeetqa ea
//
LOCUS       XP_077496339             367 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-1 chain-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077496339
VERSION     XP_077496339.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640213.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..367
                     /product="tubulin beta-1 chain-like isoform X3"
                     /calculated_mol_wt=41036
     Region          <17..354
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..367
                     /gene="LOC144107188"
                     /coded_by="XM_077640213.1:46..1149"
                     /db_xref="GeneID:144107188"
ORIGIN      
        1 mdhssgritl cspapsgqsg agnnwakghy tegaelvdsv ldvvrkeaes cdclqgfqlt
       61 hslgggtgsg mgtlliskir eeypdrimnt ysvvpspkvs dtvvepynat lsvhqlvent
      121 detycidnea lydicfrtlk lttptygdln hlvsatmsgv ttclrfpgql nadlrklavn
      181 mvpfprlhff mtgfapltsr gcqqyraltv peltqqmfda knmmaacepr hgryltvaav
      241 frgrmsmrev ddqmlniqnk dssyfvewip nnvktavcdi pprglkmsat fignstaiqe
      301 lfkriseqft amfrrkafmh wytgegmdef efteaesnln elvseyqqyq eatpedeeef
      361 eetqaea
//
LOCUS       XP_077496354             226 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain [Amblyomma americanum].
ACCESSION   XP_077496354
VERSION     XP_077496354.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640228.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..226
                     /product="tubulin beta chain"
                     /calculated_mol_wt=25777
     Region          <2..213
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..226
                     /gene="LOC144107223"
                     /coded_by="XM_077640228.1:237..917"
                     /db_xref="GeneID:144107223"
ORIGIN      
        1 mtptygdlnh lvsatmsgvt tclrfpgqln adlrklavnm vpfprlhffm tgfapltsrg
       61 cqqyraltvp eltqqmfdak nmmaacdprh gryltvaavf rgrmsmrevd dqmlniqnkd
      121 ssyfvewipn nvktavcdip prglkmsatf ignstaiqel fkriseqfta mfrrkaflhw
      181 ytgegmdefe fteaesnlne lvseyqqyqe atpedeeefe etqaea
//
LOCUS       XP_077496367             105 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107246 [Amblyomma americanum].
ACCESSION   XP_077496367
VERSION     XP_077496367.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640241.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..105
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..105
                     /product="uncharacterized protein LOC144107246"
                     /calculated_mol_wt=11363
     CDS             1..105
                     /gene="LOC144107246"
                     /coded_by="XM_077640241.1:281..598"
                     /db_xref="GeneID:144107246"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrppfa altsvvccqr pasfgpcvlp afgcvippdp
       61 fmlktkscme vvqatklhvi fardleqagk lvdgqwysls kasff
//
LOCUS       XP_077496377             222 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107251 [Amblyomma americanum].
ACCESSION   XP_077496377
VERSION     XP_077496377.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640251.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..222
                     /product="uncharacterized protein LOC144107251"
                     /calculated_mol_wt=24878
     Region          7..91
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     Region          173..207
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..222
                     /gene="LOC144107251"
                     /coded_by="XM_077640251.1:95..763"
                     /db_xref="GeneID:144107251"
ORIGIN      
        1 masfseklvq evkkheqlwd qrckvhkeag nreaawidia atlavsveec rtrwrtirdt
       61 ylkrkqhrra nakgqwnvld relgflddfl qprtrrwtlh aaapvdsrae egakrkqpee
      121 dagereaata eaaavaaava eppeplavam rpaavlphhl apggaeaqer csdpeelfcl
      181 slaahlkrll prernmakmk mlqtlhdlef gengeeaale ep
//
LOCUS       XP_077496390             183 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107253 [Amblyomma americanum].
ACCESSION   XP_077496390
VERSION     XP_077496390.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640264.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="uncharacterized protein LOC144107253"
                     /calculated_mol_wt=21438
     CDS             1..183
                     /gene="LOC144107253"
                     /coded_by="XM_077640264.1:33..584"
                     /db_xref="GeneID:144107253"
ORIGIN      
        1 mswdahvskv clslsryigi isrnkhilpa ktklnlyyaf fysyvsycfl vwgqttasni
       61 qkllvlqkri lrvianvpyd aptrpiyqqy rmipvtrmfd yrfslfyqki iaeddaffsg
      121 ivtltphqtp yvtrqrmiys lprcrtnygf stlkyilpky lntpfydtlh smsvsairnv
      181 fss
//
LOCUS       XP_077496393             253 aa            linear   INV 25-NOV-2025
DEFINITION  putative U3 small nucleolar RNA-associated protein 11 [Amblyomma
            americanum].
ACCESSION   XP_077496393
VERSION     XP_077496393.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640267.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..253
                     /product="putative U3 small nucleolar RNA-associated
                     protein 11"
                     /calculated_mol_wt=29840
     Region          13..253
                     /region_name="Utp11"
                     /note="Utp11 protein; pfam03998"
                     /db_xref="CDD:461122"
     CDS             1..253
                     /gene="LOC144107263"
                     /coded_by="XM_077640267.1:10..771"
                     /db_xref="GeneID:144107263"
ORIGIN      
        1 mssfakaaka servhkerqq pearrhfgvi ekkkddklet rdyqnkqlkl krlrrraltr
       61 npdefymhmv nsklhggehh dkvkgeeftp aqlklmqtrd lnfvttkrls eakkiarlka
      121 nlrllrvesg qvnthtffvd tkkearnfdf aeklrthpsl lcrgfnrskl etlpkeciad
      181 ipedvikeat hemkksyrel tkrlqrekel kvveqkmimk knlldkknpp vkkvkegtkd
      241 aapvylwkke rkr
//
LOCUS       XP_077496402             253 aa            linear   INV 25-NOV-2025
DEFINITION  putative U3 small nucleolar RNA-associated protein 11 [Amblyomma
            americanum].
ACCESSION   XP_077496402
VERSION     XP_077496402.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640276.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..253
                     /product="putative U3 small nucleolar RNA-associated
                     protein 11"
                     /calculated_mol_wt=29840
     Region          13..253
                     /region_name="Utp11"
                     /note="Utp11 protein; pfam03998"
                     /db_xref="CDD:461122"
     CDS             1..253
                     /gene="LOC144107269"
                     /coded_by="XM_077640276.1:102..863"
                     /db_xref="GeneID:144107269"
ORIGIN      
        1 mssfakaaka servhkerqq pearrhfgvi ekkkddklet rdyqnkqlkl krlrrraltr
       61 npdefymhmv nsklhggehh dkvkgeeftp aqlklmqtrd lnfvttkrls eakkiarlka
      121 nlrllrvesg qvnthtffvd tkkearnfdf aeklrthpsl lcrgfnrskl etlpkeciad
      181 ipedvikeat hemkksyrel tkrlqrekel kvveqkmimk knlldkknpp vkkvkegtkd
      241 aapvylwkke rkr
//
LOCUS       XP_077496406             183 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107276 [Amblyomma americanum].
ACCESSION   XP_077496406
VERSION     XP_077496406.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640280.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="uncharacterized protein LOC144107276"
                     /calculated_mol_wt=21466
     CDS             1..183
                     /gene="LOC144107276"
                     /coded_by="XM_077640280.1:8..559"
                     /db_xref="GeneID:144107276"
ORIGIN      
        1 mswdahvskv clslsryigi isrnkhilpa ktklnlyyaf fysyvsycfl vwgqttasni
       61 qkllvlqkri lrvianvpyd aptrpiyqqy rmipvtrmfd yrfslfyqki iaeddaffsg
      121 ivtltphrtp yvtrqrmiys lprcrtnygf stlkyilpky lntpfydtlh smsvsairnv
      181 fss
//
LOCUS       XP_077496430             131 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107307 [Amblyomma americanum].
ACCESSION   XP_077496430
VERSION     XP_077496430.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640304.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..131
                     /product="uncharacterized protein LOC144107307"
                     /calculated_mol_wt=14342
     Region          <71..119
                     /region_name="DUF4612"
                     /note="Domain of unknown function (DUF4612); pfam15389"
                     /db_xref="CDD:464696"
     CDS             1..131
                     /gene="LOC144107307"
                     /coded_by="XM_077640304.1:213..608"
                     /db_xref="GeneID:144107307"
ORIGIN      
        1 midemgcnmv rfmsnrvtdm ssspdtrrpn qddfcgtnda npavcqqggt srtsspiatr
       61 sthmvitlag napkkllagf vrrppsatpl rvhrerslsq ieffrvleek inrgkdfhsn
      121 dgisrcstvs a
//
LOCUS       XP_077496506             291 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107385 [Amblyomma americanum].
ACCESSION   XP_077496506
VERSION     XP_077496506.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640380.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..291
                     /product="uncharacterized protein LOC144107385"
                     /calculated_mol_wt=30153
     CDS             1..291
                     /gene="LOC144107385"
                     /coded_by="XM_077640380.1:12..887"
                     /db_xref="GeneID:144107385"
ORIGIN      
        1 meaatpagan ctagcpassa stagdledve eatsdptmlk vlrllleirq qqreilqrls
       61 rleqarqepr trspsppvss lpplcpqlpa fsiedfeaae aavrddrvaa alkkqllrlg
      121 gnnlkevaan vmgavmgvav qrlfslrgrk gkrpfvgtkl crvatdaice rqgvdilaaq
      181 gfigrwlpga cdrgggrkrr yaqaleppes haqappvnpc agsapcpgap sascpatppg
      241 mailspsgvh pssappptas ssrpatlhps sapspapppp hsllltpshp t
//
LOCUS       XP_077496553             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077496553
VERSION     XP_077496553.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640427.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like isoform X1"
                     /calculated_mol_wt=49493
     Region          1..430
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144107431"
                     /coded_by="XM_077640427.1:34..1365"
                     /db_xref="GeneID:144107431"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gavdavrsgp ygplfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrgcqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkdssy fvewipnnvk tavcdipprg
      361 lkmsatfign staiqelfkr iseqftamfr rkaflhwytg egidefefte aesnlnelvs
      421 eyqqyqeatp edeeefeetq aea
//
LOCUS       XP_077496562             442 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077496562
VERSION     XP_077496562.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640436.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..442
                     /product="tubulin beta-4 chain-like isoform X2"
                     /calculated_mol_wt=49436
     Region          1..429
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..442
                     /gene="LOC144107431"
                     /coded_by="XM_077640436.1:34..1362"
                     /db_xref="GeneID:144107431"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasgkyvp
       61 railvdlepg avdavrsgpy gplfrpdnfv fgqsgagnnw akghytegae lvdsvldvvr
      121 keaescdclq gfqlthslgg gtgsgmgtll iskireeypd rimntysvvp spkvsdtvve
      181 pynatlsvhq lventdetyc idnealydic frtlklttpt ygdlnhlvsa tmsgvttclr
      241 fpgqlnadlr klavnmvpfp rlhffmtgfa pltsrgcqqy raltvpeltq qmfdaknmma
      301 acdprhgryl tvaavfrgrm smrevddqml niqnkdssyf vewipnnvkt avcdipprgl
      361 kmsatfigns taiqelfkri seqftamfrr kaflhwytge gidefeftea esnlnelvse
      421 yqqyqeatpe deeefeetqa ea
//
LOCUS       XP_077496573             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077496573
VERSION     XP_077496573.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640447.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=49561
     Region          1..430
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144107446"
                     /coded_by="XM_077640447.1:15..1346"
                     /db_xref="GeneID:144107446"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdmep gavdafrsgp ygplfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrgcqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkdssy fvewipnnvk tavcdipprg
      361 lkmsatfign staiqelfkr iseqftamfr rkaflhwytg egmdefefte aesnlnelvs
      421 eyqqfqeatp edeeefeetq aea
//
LOCUS       XP_077496585              93 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4B chain-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077496585
VERSION     XP_077496585.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640459.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..93
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..93
                     /product="tubulin beta-4B chain-like isoform X1"
                     /calculated_mol_wt=10163
     Region          1..>92
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..93
                     /gene="LOC144107456"
                     /coded_by="XM_077640459.1:232..513"
                     /db_xref="GeneID:144107456"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gavdavrsgp ygplfrpdnf vfe
//
LOCUS       XP_077496594              92 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4B chain-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077496594
VERSION     XP_077496594.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640468.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..92
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..92
                     /product="tubulin beta-4B chain-like isoform X2"
                     /calculated_mol_wt=10106
     Region          1..>91
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..92
                     /gene="LOC144107456"
                     /coded_by="XM_077640468.1:232..510"
                     /db_xref="GeneID:144107456"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasgkyvp
       61 railvdlepg avdavrsgpy gplfrpdnfv fe
//
LOCUS       XP_077496606             469 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase RNF14-like [Amblyomma americanum].
ACCESSION   XP_077496606
VERSION     XP_077496606.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640480.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..469
                     /product="E3 ubiquitin-protein ligase RNF14-like"
                     /calculated_mol_wt=53489
     Region          7..132
                     /region_name="RWD_RNF14"
                     /note="RWD domain of RING finger protein 14 (RNF14) and
                     related proteins; cd23820"
                     /db_xref="CDD:467656"
     Region          205..263
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(212,215,230,232,235,238,257,262)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438290"
     Region          292..343
                     /region_name="BRcat_RBR_RNF14"
                     /note="BRcat domain found in RING finger protein 14
                     (RNF14); cd20341"
                     /db_xref="CDD:439002"
     Region          381..449
                     /region_name="Rcat_RBR_RNF14"
                     /note="Rcat domain found in RING finger protein 14
                     (RNF14); cd20354"
                     /db_xref="CDD:439015"
     CDS             1..469
                     /gene="LOC144107464"
                     /coded_by="XM_077640480.1:282..1691"
                     /db_xref="GeneID:144107464"
ORIGIN      
        1 medseaqdne llalesiydd svfqavkkqg ccpeghflis velpkpfcvl vprvdapgem
       61 ekvtlehlpp irvdfelpan ypsesvpkic vscpwltsre lavvcnaldr swesfprsvv
      121 lfrwitllds eaanilelks thslaalnre ltrrranpss irwkkcysyr ghgshdlrcf
      181 kevvdphslg tfltefniea krrafdrqwl mcqvcltskl gtqfelvigc nhpfcrqclc
      241 efvriqiesg casqvrcpqe gcdtrfvptq vtslvgrdlg lryeeslfna yldsqddmty
      301 cprlpcqrav vmdpdvptat catclfsfcv lcrqlyhgve pckqhpggqr airdqylaag
      361 psgkkvmekl ygkrtlqrmi demltvdwme ensrkcphcr ifiekldgcn kmtcwrcgth
      421 fcwicmvaiq ksgnpyehfs nasskcfnkl fdgaeqpfve eqhdfgefl
//
LOCUS       XP_077496637             239 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107490 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077496637
VERSION     XP_077496637.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640511.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..239
                     /product="uncharacterized protein LOC144107490 isoform X1"
                     /calculated_mol_wt=26570
     Region          67..143
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..239
                     /gene="LOC144107490"
                     /coded_by="XM_077640511.1:517..1236"
                     /db_xref="GeneID:144107490"
ORIGIN      
        1 mtmdegaqrl fmtmieespl paapavvdal atlvnvgeip dentaasdvg ergpqpapev
       61 krgggapewt pgetkllldy cykyfpqvgp fkkfknkkml fkqvsqdlae vlgcnktpqq
      121 cenryktvmr qkkmasdnnn ksgaspqpvp fddemrkiqs iddslepeiq rdsfgvthks
      181 tssssdssad lispmpempr tlssnsende tmkrstevkk rkcrastsrm qqmqfffik
//
LOCUS       XP_077496639             213 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107490 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077496639
VERSION     XP_077496639.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640513.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..213
                     /product="uncharacterized protein LOC144107490 isoform X2"
                     /calculated_mol_wt=23861
     Region          41..117
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..213
                     /gene="LOC144107490"
                     /coded_by="XM_077640513.1:133..774"
                     /db_xref="GeneID:144107490"
ORIGIN      
        1 mgfqgfqgpl rtllaentaa sdvgergpqp apevkrggga pewtpgetkl lldycykyfp
       61 qvgpfkkfkn kkmlfkqvsq dlaevlgcnk tpqqcenryk tvmrqkkmas dnnnksgasp
      121 qpvpfddemr kiqsiddsle peiqrdsfgv thkstssssd ssadlispmp emprtlssns
      181 endetmkrst evkkrkcras tsrmqqmqff fik
//
LOCUS       XP_077496663             243 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107506 [Amblyomma americanum].
ACCESSION   XP_077496663
VERSION     XP_077496663.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640537.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..243
                     /product="uncharacterized protein LOC144107506"
                     /calculated_mol_wt=27516
     Region          <37..214
                     /region_name="transpos_IS1595"
                     /note="IS1595 family transposase; NF033547"
                     /db_xref="CDD:468078"
     CDS             1..243
                     /gene="LOC144107506"
                     /coded_by="XM_077640537.1:40..771"
                     /db_xref="GeneID:144107506"
ORIGIN      
        1 mdnilygegp hfypdtgarv egfqaeerrq erlanyirev leqelqarpp mggvgqevqi
       61 detllrgkrk anrgrlltgd avpprhrnny ggvadkgpwv fgmvwsqtge lrlfqvekrd
      121 aatlgpiiaa nimpgttvys dewaayncip slqdgnvapl nldwhtvnht tnfvdpltga
      181 ntqriesawq kvkrklvrng qktsrvllqs hlswqwwqsi ngrtkcqdpf lrlteaiarr
      241 ypl
//
LOCUS       XP_077496680             105 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107514 [Amblyomma americanum].
ACCESSION   XP_077496680
VERSION     XP_077496680.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640554.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..105
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..105
                     /product="uncharacterized protein LOC144107514"
                     /calculated_mol_wt=12118
     CDS             1..105
                     /gene="LOC144107514"
                     /coded_by="XM_077640554.1:246..563"
                     /db_xref="GeneID:144107514"
ORIGIN      
        1 mpcafqdlvf llqehvasvv lvfknlqlyy ddphkvrfed rrrrhlnrrl tatmkrlsgv
       61 hvlnpehrfl dasdepmlsl faadryhvar rrgidlcsfa vaspg
//
LOCUS       XP_077496709             144 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107532 [Amblyomma americanum].
ACCESSION   XP_077496709
VERSION     XP_077496709.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640583.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..144
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..144
                     /product="uncharacterized protein LOC144107532"
                     /calculated_mol_wt=15819
     Region          <20..>108
                     /region_name="PRK10547"
                     /note="chemotaxis protein CheA; Provisional"
                     /db_xref="CDD:236712"
     CDS             1..144
                     /gene="LOC144107532"
                     /coded_by="XM_077640583.1:260..694"
                     /db_xref="GeneID:144107532"
ORIGIN      
        1 mlsrptsgnl eplphaeavy ivpeenisdt pqgsaeaifq qmfvesaslp sspgtsaapt
       61 pnspsasvel vaempstgsr kkrsriekyd sqidmlgeml kkkpdsldhf gaylvdkmre
      121 vpkrlhnemq mkllqcafef ipek
//
LOCUS       XP_077496729             233 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107541 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077496729
VERSION     XP_077496729.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640603.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..233
                     /product="uncharacterized protein LOC144107541 isoform X1"
                     /calculated_mol_wt=26083
     Region          <33..180
                     /region_name="TesA"
                     /note="Lysophospholipase L1 or related esterase. Includes
                     spore coat protein LipC/YcsK [Cell cycle control, cell
                     division, chromosome partitioning, Lipid transport and
                     metabolism]; COG2755"
                     /db_xref="CDD:442045"
     CDS             1..233
                     /gene="LOC144107541"
                     /coded_by="XM_077640603.1:163..864"
                     /db_xref="GeneID:144107541"
ORIGIN      
        1 maflrgalvg dsqlkflcss rlrlsplvrt ctfsfsghda islaemiret cfrhadfvvl
       61 yvggndldrg ddpreiashi kdlvlllqen vasvvlvfkv lprhfdeprk aqfedrrrrq
      121 lnrrlsatlk rlsgvhilnp ehrfldasgk pmlslfaadr yhvardrgid qmskiivaal
      181 vkiygpgias asrarpgevy vvhrcrrcga kghktdhcwa ycsprrhaaa grg
//
LOCUS       XP_077496737             226 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107541 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077496737
VERSION     XP_077496737.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640611.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..226
                     /product="uncharacterized protein LOC144107541 isoform X2"
                     /calculated_mol_wt=25288
     Region          <33..173
                     /region_name="TesA"
                     /note="Lysophospholipase L1 or related esterase. Includes
                     spore coat protein LipC/YcsK [Cell cycle control, cell
                     division, chromosome partitioning, Lipid transport and
                     metabolism]; COG2755"
                     /db_xref="CDD:442045"
     CDS             1..226
                     /gene="LOC144107541"
                     /coded_by="XM_077640611.1:163..843"
                     /db_xref="GeneID:144107541"
ORIGIN      
        1 maflrgalvg dsqlkflcss rlrlsplvrt ctfsfsghda islaemiret cfrhadfvvl
       61 yvggndldrg ddpreiashi kenvasvvlv fkvlprhfde prkaqfedrr rrqlnrrlsa
      121 tlkrlsgvhi lnpehrflda sgkpmlslfa adryhvardr gidqmskiiv aalvkiygpg
      181 iasasrarpg evyvvhrcrr cgakghktdh cwaycsprrh aaagrg
//
LOCUS       XP_077496747             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107541 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077496747
VERSION     XP_077496747.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640621.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144107541 isoform X3"
                     /calculated_mol_wt=17738
     CDS             1..158
                     /gene="LOC144107541"
                     /coded_by="XM_077640621.1:459..935"
                     /db_xref="GeneID:144107541"
ORIGIN      
        1 mscafqdlvl llqenvasvv lvfkvlprhf deprkaqfed rrrrqlnrrl satlkrlsgv
       61 hilnpehrfl dasgkpmlsl faadryhvar drgidqmski ivaalvkiyg pgiasasrar
      121 pgevyvvhrc rrcgakghkt dhcwaycspr rhaaagrg
//
LOCUS       XP_077496758             126 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107559 [Amblyomma americanum].
ACCESSION   XP_077496758
VERSION     XP_077496758.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640632.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..126
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..126
                     /product="uncharacterized protein LOC144107559"
                     /calculated_mol_wt=12957
     CDS             1..126
                     /gene="LOC144107559"
                     /coded_by="XM_077640632.1:5..385"
                     /db_xref="GeneID:144107559"
ORIGIN      
        1 mrqavgaarp cvqaavarpr arhhssdvff lqpepvpcvr rggtgderpa agrgalsicy
       61 tgaascgagp sesaaaeyag gggapaasgd pahqrpprml rtsrfthasw stkarqqhks
      121 aasrrq
//
LOCUS       XP_077496769             306 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107567 [Amblyomma americanum].
ACCESSION   XP_077496769
VERSION     XP_077496769.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640643.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..306
                     /product="uncharacterized protein LOC144107567"
                     /calculated_mol_wt=35484
     Region          <63..>192
                     /region_name="COG2433"
                     /note="Possible nuclease of RNase H fold, RuvC/YqgF family
                     [General function prediction only]"
                     /db_xref="CDD:441980"
     Region          117..>165
                     /region_name="HOOK"
                     /note="HOOK protein coiled-coil region; pfam05622"
                     /db_xref="CDD:461694"
     CDS             1..306
                     /gene="LOC144107567"
                     /coded_by="XM_077640643.1:364..1284"
                     /db_xref="GeneID:144107567"
ORIGIN      
        1 mpldehcgsc dmqleeldsk idcsdcrsvy htgkcagisd atikskgeqy rsawrcsncr
       61 rskgrtpqld rssndtddqd vrallksine kleyllplke tvdsvedtvq ymsdqydell
      121 trveknerev relkhrvdki ekqddeitql knemdclewr srklnlefhg ikamedenli
      181 deinklatln nlpqlqesdi vsthrlpskq dkipgiicrf akqsvrdkww qnrktlcqgn
      241 esifglenlt krtrtlltet klwakannyk yvwhnnnkvl vrkadgqnav viscssdrdk
      301 lkqqis
//
LOCUS       XP_077496854             156 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107624 [Amblyomma americanum].
ACCESSION   XP_077496854
VERSION     XP_077496854.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640728.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..156
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..156
                     /product="uncharacterized protein LOC144107624"
                     /calculated_mol_wt=17517
     CDS             1..156
                     /gene="LOC144107624"
                     /coded_by="XM_077640728.1:72..542"
                     /db_xref="GeneID:144107624"
ORIGIN      
        1 mclmkaaifs glilalffla spstgnhswg rlrkvikmpt ekekftmaee crapacqqnc
       61 vdayhkyydv igncegldci cefkkpctir ycynkcmtky qnetkvgltg tcertncvcd
      121 wgnkcdkakc kdscvtlhgk gtkakcvred cvcrkk
//
LOCUS       XP_077496873             129 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107646 [Amblyomma americanum].
ACCESSION   XP_077496873
VERSION     XP_077496873.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640747.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..129
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..129
                     /product="uncharacterized protein LOC144107646"
                     /calculated_mol_wt=14874
     CDS             1..129
                     /gene="LOC144107646"
                     /coded_by="XM_077640747.1:7..396"
                     /db_xref="GeneID:144107646"
ORIGIN      
        1 mvkvlprwfi tadaevsrsw lnrrhllnrk ltatlkrmps vfilnpdhrf learkfpkrh
       61 lfgtdvyhls agegveelve ilvrslekvy gpgilapfsr vptrhiinac hhcgtkdhvn
      121 gdfyeycrp
//
LOCUS       XP_077496957             418 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107698 [Amblyomma americanum].
ACCESSION   XP_077496957
VERSION     XP_077496957.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640831.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..418
                     /product="uncharacterized protein LOC144107698"
                     /calculated_mol_wt=45556
     CDS             1..418
                     /gene="LOC144107698"
                     /coded_by="XM_077640831.1:27..1283"
                     /db_xref="GeneID:144107698"
ORIGIN      
        1 meedscydls llrvlrssht pqpvtsglse vidkepapls paadvdtdql qasstcndga
       61 gpvttgtast lpvapaafes tqgplirvdn aapaqraasd plqyfligil faglfvaaal
      121 vvigapsgpl ssydlrseqk rrpaqpplmp rydiqdvade nlfvviprnd qppqhvvqda
      181 padgalarpg dfsvtgaaas eeapqyagav dsmpserrvk gdeqcsryyy tycskpvtvf
      241 hfdpeqgacv paaenraqlc nrgsnrfgsw dqcrqsclep ervsdkcfen tlfvpcftqd
      301 mvaafwffng ttcttwgfph gycpslpygv yrtfdecsrr cgpndrsppk vemvhcavpe
      361 arpctlsqlq fpyfalmrga rgrarcvnts rerllsrrcv vgsnqfrsla scqkacly
//
LOCUS       XP_077496967             130 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107707 [Amblyomma americanum].
ACCESSION   XP_077496967
VERSION     XP_077496967.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640841.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..130
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..130
                     /product="uncharacterized protein LOC144107707"
                     /calculated_mol_wt=13363
     CDS             1..130
                     /gene="LOC144107707"
                     /coded_by="XM_077640841.1:190..582"
                     /db_xref="GeneID:144107707"
ORIGIN      
        1 mldnnearkt teaggedngl iqkkidvpgl vvtpeatprl rslrsvtvvh eaftsaspcv
       61 adkedsratl tflvvfalml glsiggtlav lflvhlasrg vsvngttsev qpaeganast
      121 ggsdaswigr
//
LOCUS       XP_077496987             438 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107723 [Amblyomma americanum].
ACCESSION   XP_077496987
VERSION     XP_077496987.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640861.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..438
                     /product="uncharacterized protein LOC144107723"
                     /calculated_mol_wt=46175
     Region          <230..>318
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     Region          <324..408
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..438
                     /gene="LOC144107723"
                     /coded_by="XM_077640861.1:224..1540"
                     /db_xref="GeneID:144107723"
ORIGIN      
        1 malpeqrgyd pttlswssvp asqpedgpst isaqqaalfq vvesrrrrrq nargaatpkt
       61 pldksatgda rapsskgpqk sfspvskwtp kptvrmnpad yvivlrprst valktafqqg
      121 elgaaiseli grqhmnaiti spnweqniiv cgtqnpevaq klltdiqitt skgslplhgh
      181 lkltgdvcrg visvhnlets eslkhsvqwr ggdlvhvrkl gksnvavltf egrsvpryvh
      241 ynaeltpvke ykktvpacyr cgtlghrvdn cptpvkercg lcgqnvglgt egmaphvcnp
      301 mclicggphl tssrectakf iklpqpgprt pskgansgat rggrqqnnqk tprrgvmppp
      361 gktatvtppp gaaefpalga spggaacnqp krglgggpar lsrpeepkgl gstdaggvac
      421 qwgpgigapp stvrgkgq
//
LOCUS       XP_077497019             100 aa            linear   INV 25-NOV-2025
DEFINITION  polyhomeotic-like protein 3 [Amblyomma americanum].
ACCESSION   XP_077497019
VERSION     XP_077497019.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640893.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..100
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..100
                     /product="polyhomeotic-like protein 3"
                     /calculated_mol_wt=10913
     Region          26..91
                     /region_name="SAM_superfamily"
                     /note="SAM (Sterile alpha motif); cl15755"
                     /db_xref="CDD:472832"
     CDS             1..100
                     /gene="LOC144107738"
                     /coded_by="XM_077640893.1:467..769"
                     /db_xref="GeneID:144107738"
ORIGIN      
        1 mvnqeelnqe kvqnlldhla llaaenprcw tvasaiayia tipsctqcve efsaqvankk
       61 alrlltedyl intmgmtigm alkvchvirs lipvmghttt
//
LOCUS       XP_077497043             409 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107754 [Amblyomma americanum].
ACCESSION   XP_077497043
VERSION     XP_077497043.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640917.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..409
                     /product="uncharacterized protein LOC144107754"
                     /calculated_mol_wt=44750
     Region          153..173
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(153,156,169,173)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          <175..258
                     /region_name="SFP1"
                     /note="Putative transcriptional repressor regulating G2/M
                     transition [Transcription / Cell division and chromosome
                     partitioning]; COG5189"
                     /db_xref="CDD:227516"
     Region          181..201
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(181,184,197,201)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          209..229
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(209,212,225,229)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(214,216,218,220..221,224..225,228,242,244,248..249,
                     252..253,256,270,272,274,276..277,280..281,284)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          237..257
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          249..274
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          265..285
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          293..313
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          321..341
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..409
                     /gene="LOC144107754"
                     /coded_by="XM_077640917.1:158..1387"
                     /db_xref="GeneID:144107754"
ORIGIN      
        1 maanrrgqkd lvspsptpld fcsmpvlyec vvgteptpss lssdtpeaga rpesaavdsv
       61 vesapnssqi ahaesnppah cvacgrhfrm hvqlrnhmvl haplrsavpa akaedvtmpd
      121 pprqvapppl pslpgsrrgs snqssvekma hscyicskkf kqvghlnnhv rlhtgekpye
      181 cqvcnkkftq sghlishtrm htnqrpyeck hcnksftqsg hlsnherlhs gerpyecnvc
      241 skrftqsghl snhmrvhsge rpfecevcsk sftqsghlnn hmrlhtggkp hfcpmcfkrf
      301 aqtghlnnhi rvhsgerpfg cyicgktfvq sghltshvrt hqghsagnss gscsvqkgat
      361 ssqrpaphii rlspapqvdl mespptlaev tspgesrket geepadvrl
//
LOCUS       XP_077497054             100 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107762 [Amblyomma americanum].
ACCESSION   XP_077497054
VERSION     XP_077497054.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640928.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..100
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..100
                     /product="uncharacterized protein LOC144107762"
                     /calculated_mol_wt=10966
     CDS             1..100
                     /gene="LOC144107762"
                     /coded_by="XM_077640928.1:107..409"
                     /db_xref="GeneID:144107762"
ORIGIN      
        1 mlrstvmdrq pssaplqave nvqplalslp kgpwtkqlkg lhrsfaddsi lqheralsaf
       61 khcvagyrmf kaekvhdall ceaggqcpcq sssrmqcqrs
//
LOCUS       XP_077497088             283 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107780 [Amblyomma americanum].
ACCESSION   XP_077497088
VERSION     XP_077497088.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640962.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..283
                     /product="uncharacterized protein LOC144107780"
                     /calculated_mol_wt=31385
     Region          11..35
                     /region_name="zf-U11-48K"
                     /note="U11-48K-like CHHC zinc finger; pfam05253"
                     /db_xref="CDD:461603"
     CDS             1..283
                     /gene="LOC144107780"
                     /coded_by="XM_077640962.1:66..917"
                     /db_xref="GeneID:144107780"
ORIGIN      
        1 matyrsthep lltcpfnpah qmkrgrylih iskckrshrg egmkrcpfsp dhevephkim
       61 hhiytcplnk avnyteppsd kdvpvmkril aldgskctei tenwdselad rcelqasrta
      121 tagqrqlrgd searepglqc pgtsslqsat qsshpeally qqgdrglhcg arprtpldmh
      181 aahrrfhqdr sevprqptse pkviqslkmk qhqalegwgn hqsigamvtp glasvpaaap
      241 lsaahgvvhs qfetplasal waeeeeaclr mrllgigrgr rln
//
LOCUS       XP_077497097             144 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107789 [Amblyomma americanum].
ACCESSION   XP_077497097
VERSION     XP_077497097.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640971.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..144
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..144
                     /product="uncharacterized protein LOC144107789"
                     /calculated_mol_wt=15478
     CDS             1..144
                     /gene="LOC144107789"
                     /coded_by="XM_077640971.1:452..886"
                     /db_xref="GeneID:144107789"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrppfa altsviccqr pasigpcvlp afgcvippdp
       61 fmlktkshme vvqatklrvi fardleqagk lvdgqwysls kasffsktkt islgswamyl
      121 gtcgqdnagq hasasskkka rnsk
//
LOCUS       XP_077497124             210 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107802 [Amblyomma americanum].
ACCESSION   XP_077497124
VERSION     XP_077497124.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077640998.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..210
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..210
                     /product="uncharacterized protein LOC144107802"
                     /calculated_mol_wt=23472
     Region          4..94
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     Region          <112..203
                     /region_name="KLF6_7_N-like"
                     /note="N-terminal domain of Kruppel-like factor (KLF) 6,
                     KLF7, and similar proteins; cl41732"
                     /db_xref="CDD:425363"
     CDS             1..210
                     /gene="LOC144107802"
                     /coded_by="XM_077640998.1:36..668"
                     /db_xref="GeneID:144107802"
ORIGIN      
        1 meafletvrg ypclydklni dfkdkdlrai rwhmigqqfg mtgeqaagkf knfrdrwlkv
       61 alekkkayks gapgkegkak sewtyyyild sflrktpyya eklttniplp qerhlqqaee
      121 alstssaagd cwpspspaee vldeicsgrt tmcnsdeesc gllsptptet eassgsnsgt
      181 nraqppaqps qvrlsstpqs rkrkkndtfe
//
LOCUS       XP_077497144             285 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107815 [Amblyomma americanum].
ACCESSION   XP_077497144
VERSION     XP_077497144.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641018.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..285
                     /product="uncharacterized protein LOC144107815"
                     /calculated_mol_wt=32031
     Region          59..>152
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     CDS             1..285
                     /gene="LOC144107815"
                     /coded_by="XM_077641018.1:19..876"
                     /db_xref="GeneID:144107815"
ORIGIN      
        1 mqvgvpytlf akkssnyclv qlpspqccfc laiecvnvir sllllsgdve tnpgppntda
       61 vlaelqklsa gqrklisevq glknqlvttd ktisalseri anletqfevi stlrteietl
      121 qtstartthq lseidtrldn lenhsrrndl vfyglpdpne kesydqseni iiqrcldsln
      181 lvidpknier ahrlgrhsid rqrpiivkls afktkgvils hgpklkntdf sigedfsrpv
      241 rnarkhlvaf artksgpfsl ryktlhmgpk rymfdqdtqt vkeia
//
LOCUS       XP_077497169             205 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107832 [Amblyomma americanum].
ACCESSION   XP_077497169
VERSION     XP_077497169.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641043.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..205
                     /product="uncharacterized protein LOC144107832"
                     /calculated_mol_wt=21875
     CDS             1..205
                     /gene="LOC144107832"
                     /coded_by="XM_077641043.1:103..720"
                     /db_xref="GeneID:144107832"
ORIGIN      
        1 mermpfawcg lssmeivdrs sgtcagrdcw pgrrsdaafr laardssdpk pveclrlllf
       61 cswivipava dkrvgvktsv delhplgmlc lvldysvaws hleaiggvgg larslprgvi
      121 itvagsgais lvvketgfel wltahlkrst agllvkqsil avsasiitel vsnssalsll
      181 lpvavdvvsi whtlyskeyt lalll
//
LOCUS       XP_077497212             182 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107867 [Amblyomma americanum].
ACCESSION   XP_077497212
VERSION     XP_077497212.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641086.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..182
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..182
                     /product="uncharacterized protein LOC144107867"
                     /calculated_mol_wt=18849
     CDS             1..182
                     /gene="LOC144107867"
                     /coded_by="XM_077641086.1:99..647"
                     /db_xref="GeneID:144107867"
ORIGIN      
        1 msrcsstttv efvegtrpps psvcimsesr ppspsvcivs esrppspsvc ivsesrpptp
       61 slcivsesrp psppavcvvk seqrepsfcv vsgkrtsspt tvdvvgsggg cprrvpsrsp
      121 nvcivtetap pppppsdvci vtqgrspslc vvtgessrrs pttvevigsc krststveii
      181 qd
//
LOCUS       XP_077497223             182 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107874 [Amblyomma americanum].
ACCESSION   XP_077497223
VERSION     XP_077497223.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641097.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..182
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..182
                     /product="uncharacterized protein LOC144107874"
                     /calculated_mol_wt=18892
     CDS             1..182
                     /gene="LOC144107874"
                     /coded_by="XM_077641097.1:233..781"
                     /db_xref="GeneID:144107874"
ORIGIN      
        1 msrcsstttv efvegtrpps psvcimsesr ppspsvcivs esrppspsvc ivsesrpptp
       61 slcivsesrp psppavcvvk seqrepsfcv vsgkrtsspt tvyvvgsggg cprrvpsrsp
      121 nvcivtetap pppppsdvci vthgrspslc vvtgessrrs pttvevigsc krssstveii
      181 qd
//
LOCUS       XP_077497255             187 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107894 [Amblyomma americanum].
ACCESSION   XP_077497255
VERSION     XP_077497255.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641129.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="uncharacterized protein LOC144107894"
                     /calculated_mol_wt=21193
     CDS             1..187
                     /gene="LOC144107894"
                     /coded_by="XM_077641129.1:34..597"
                     /db_xref="GeneID:144107894"
ORIGIN      
        1 mrerrkikar sgaspdeiyq pkwahykslm fldsscssqp sfstlhtqee eeeterqceh
       61 wnnesadhem dfpcspqnca epslpaqqig egtsrpgkkr rrndeeteqr kvllttavet
      121 lqrsqqrlaa ndecdaigsm mahavrqipp gpdrqlamln ahqaivkinl srhsvpveml
      181 nvlnmqe
//
LOCUS       XP_077497301             176 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107934 [Amblyomma americanum].
ACCESSION   XP_077497301
VERSION     XP_077497301.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641175.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..176
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..176
                     /product="uncharacterized protein LOC144107934"
                     /calculated_mol_wt=19012
     CDS             1..176
                     /gene="LOC144107934"
                     /coded_by="XM_077641175.1:20..550"
                     /db_xref="GeneID:144107934"
ORIGIN      
        1 mavqpsqlsp senivvdptp sahssprdkk pqqikdpise dapqhleanp gyittrqgtl
       61 taletvgaaw lmvlyllmgs sitwyahaaf llaftyclnn tliiisamas pstqrnlplt
      121 lfyslyhcaa gllflmgaav fysfrsddlp ykgfmgvtlf gvgtlhvchg lsnlam
//
LOCUS       XP_077497312             143 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107942 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077497312
VERSION     XP_077497312.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641186.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..143
                     /product="uncharacterized protein LOC144107942 isoform X1"
                     /calculated_mol_wt=15619
     CDS             1..143
                     /gene="LOC144107942"
                     /coded_by="XM_077641186.1:101..532"
                     /db_xref="GeneID:144107942"
ORIGIN      
        1 mdfdleaaqk slklditfkd yvatsnglfn faetvggial fftmslsglg tntesflyav
       61 syaystngiq illagflnhs savivqrlff yathqcfgtl ayvsgammlv sdednltmag
      121 mismvvgach ffhlihlffk iyv
//
LOCUS       XP_077497319             100 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107942 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077497319
VERSION     XP_077497319.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641193.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..100
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..100
                     /product="uncharacterized protein LOC144107942 isoform X2"
                     /calculated_mol_wt=10887
     CDS             1..100
                     /gene="LOC144107942"
                     /coded_by="XM_077641193.1:112..414"
                     /db_xref="GeneID:144107942"
ORIGIN      
        1 mslsglgtnt esflyavsya ystngiqill agflnhssav ivqrlffyat hqcfgtlayv
       61 sgammlvsde dnltmagmis mvvgachffh lihlffkiyv
//
LOCUS       XP_077497327              87 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107942 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077497327
VERSION     XP_077497327.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641201.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..87
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..87
                     /product="uncharacterized protein LOC144107942 isoform X3"
                     /calculated_mol_wt=9628
     CDS             1..87
                     /gene="LOC144107942"
                     /coded_by="XM_077641201.1:101..364"
                     /db_xref="GeneID:144107942"
ORIGIN      
        1 mdfdleaaqk slklditfkd yvatsnglfn faetyathqc fgtlayvsga mmlvsdednl
       61 tmagmismvv gachffhlih lffkiyv
//
LOCUS       XP_077497350             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107967 [Amblyomma americanum].
ACCESSION   XP_077497350
VERSION     XP_077497350.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641224.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144107967"
                     /calculated_mol_wt=13360
     CDS             1..127
                     /gene="LOC144107967"
                     /coded_by="XM_077641224.1:124..507"
                     /db_xref="GeneID:144107967"
ORIGIN      
        1 mstsssgqgs sswsaclspd gdstvlnnte aveaalrsss phcmrmtevr efgraafvyr
       61 spdeacvadl ivctscashp msafvpphla cskgiqqets wpafctavly sdisidptgl
      121 fsvlevf
//
LOCUS       XP_077497359             415 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144107974 [Amblyomma americanum].
ACCESSION   XP_077497359
VERSION     XP_077497359.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641233.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..415
                     /product="uncharacterized protein LOC144107974"
                     /calculated_mol_wt=46761
     Region          1..355
                     /region_name="transpos_IS630"
                     /note="IS630 family transposase; NF033545"
                     /db_xref="CDD:468076"
     CDS             1..415
                     /gene="LOC144107974"
                     /coded_by="XM_077641233.1:27..1274"
                     /db_xref="GeneID:144107974"
ORIGIN      
        1 mtgvsprtvv rfkkeglsgg vkspekrpkr maisssrsvk ydsftvnair lklhrmyaer
       61 qiptldsilh evngdddlpd mskatlwrll kemgfkfekr krtlaliers divawrrryl
      121 raikafrsqg rcivyldetw vnaghtrqhv wkdstvkssq daflrglttg lpapsgkggr
      181 lilvhagssl tgfvsgaadf frakkgasad yhsemngkyf eewfsskllp nipnssiivm
      241 dnapyhsval ekppttstrk ieiqawltrk giswstdmvr aelldlckrt tsghtvyvid
      301 tlsakhghev lrlppyhcef npieqvwsmv kgfvakrnka ftlaeveell pqalahvske
      361 dwqkfcahae riedeawerd iifddevdpv vfaigsssss seessselsg ieele
//
LOCUS       XP_077497393             294 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108004 [Amblyomma americanum].
ACCESSION   XP_077497393
VERSION     XP_077497393.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641267.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..294
                     /product="uncharacterized protein LOC144108004"
                     /calculated_mol_wt=32358
     CDS             1..294
                     /gene="LOC144108004"
                     /coded_by="XM_077641267.1:1..885"
                     /db_xref="GeneID:144108004"
ORIGIN      
        1 mdteaalriw kmthmyatpl kyttflsdgd skayaavaev diygsvsvqk edctnhiakr
       61 lgtalrkakf prggklkdkt iaklqgyfqv aitsnrgnvr dmycavwasy fhscsrdgas
      121 shkfcpdgem srckhkrala fgqpapvhtp ilsvsqgkav lliykrltdk kllqrcvkgr
      181 tqnaagslns kiwllcpktk fatrtvveta taiavlwfnk ghtgfeevlq elgilpskal
      241 lslskevdkr rissmstkat aearahprnv tkkarleesa hrdcegptyg agaf
//
LOCUS       XP_077497423             238 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108024 [Amblyomma americanum].
ACCESSION   XP_077497423
VERSION     XP_077497423.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641297.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..238
                     /product="uncharacterized protein LOC144108024"
                     /calculated_mol_wt=27065
     Region          28..209
                     /region_name="transpos_IS1595"
                     /note="IS1595 family transposase; NF033547"
                     /db_xref="CDD:468078"
     CDS             1..238
                     /gene="LOC144108024"
                     /coded_by="XM_077641297.1:1995..2711"
                     /db_xref="GeneID:144108024"
ORIGIN      
        1 makgltstqt relvlkdfkl rndarsdwrn yirevleqel qarppmggvg qevqidetll
       61 rgkrkanrgr lltgdavppr hrnnyggvad kgpwvfgmvw sqtgelrlfq vekrdaatlg
      121 piiaaniqpc ttvysdewaa yncipslqdg ngtslnldwh tvnhttnfvd pltgantqri
      181 esawqkvkrk lvrnvqktsr allqshlswq wwqsingrtk cqdpflrlte aiawrypl
//
LOCUS       XP_077497445             221 aa            linear   INV 25-NOV-2025
DEFINITION  polyhomeotic-like protein 2 [Amblyomma americanum].
ACCESSION   XP_077497445
VERSION     XP_077497445.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641319.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..221
                     /product="polyhomeotic-like protein 2"
                     /calculated_mol_wt=23973
     Region          155..220
                     /region_name="SAM_superfamily"
                     /note="SAM (Sterile alpha motif); cl15755"
                     /db_xref="CDD:472832"
     CDS             1..221
                     /gene="LOC144108036"
                     /coded_by="XM_077641319.1:120..785"
                     /db_xref="GeneID:144108036"
ORIGIN      
        1 mtirnaakea vrsvqdtlqd dqqppevstl saiflntpdq perneaavvv idghstrrgs
       61 sstggprpee vesvdpaeyl htvfavlead yvsdphvyqa tahsartrpe dsslrhltpe
      121 tarndvaesn pgenivsqer mehllehlal laaenprgwt vadaiayiat ipsctgcvee
      181 fsaqitgrea lfllnedyli ntmgltlgla lkvcdvvrsl i
//
LOCUS       XP_077497456             426 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108046 [Amblyomma americanum].
ACCESSION   XP_077497456
VERSION     XP_077497456.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641330.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..426
                     /product="uncharacterized protein LOC144108046"
                     /calculated_mol_wt=47392
     Region          <218..273
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     CDS             1..426
                     /gene="LOC144108046"
                     /coded_by="XM_077641330.1:1425..2705"
                     /db_xref="GeneID:144108046"
ORIGIN      
        1 mqgflkapvp spcwapwwaa staaehqtff mapplskpdr shkrvrtedi tnqcfrktke
       61 sfpkfhvlhs eeteksartm spflvskylt daigpgykls kmpngdllle ikdidqyskl
      121 snqvqigdni vsitahrsln tvrgvisetd fihlteeeml eglkdqnvin vhrikirked
      181 keiptkhivl tfncstlpes ieagylkinv rnyiphprrc fncqryghgs qscrghktca
      241 kcaskdhase ncegaafrca ncgddhpays rscqtwknen diitmktkqn itfkearrlf
      301 yakntftfst kttfadvvrr ggaphstpas akatlsepma kpptpqteta kaalsspkqg
      361 dpsngsasrr tspqrgrpkn ltsaasrsss tslevmditp spsalkrrns slerkkgrpl
      421 itgpek
//
LOCUS       XP_077497467             263 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108057 [Amblyomma americanum].
ACCESSION   XP_077497467
VERSION     XP_077497467.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641341.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..263
                     /product="uncharacterized protein LOC144108057"
                     /calculated_mol_wt=30623
     Region          108..196
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     CDS             1..263
                     /gene="LOC144108057"
                     /coded_by="XM_077641341.1:1..792"
                     /db_xref="GeneID:144108057"
ORIGIN      
        1 myrmpptrrd pspdrkentn vvpdnrasrr lqqlppehgl lpkktkkiva eatpmaapas
       61 pivlqqprep ptfrgstfed peswletyer vttfnswasd dklrhvyfal edaartwfen
      121 reatlmtwdl frssflqtft svlrkeqaqa lletraqlln etiaifteem srlfrhadpe
      181 myeekklrll mrgvkeelfg amirspptti eeflreatsi ektlemrnrq fnrrtnstny
      241 agvqslatdd lretiravvr eel
//
LOCUS       XP_077497477             180 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108067 [Amblyomma americanum].
ACCESSION   XP_077497477
VERSION     XP_077497477.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641351.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..180
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..180
                     /product="uncharacterized protein LOC144108067"
                     /calculated_mol_wt=19519
     Region          <4..38
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..180
                     /gene="LOC144108067"
                     /coded_by="XM_077641351.1:71..613"
                     /db_xref="GeneID:144108067"
ORIGIN      
        1 mtaariqrwa lylrgynykl qyvlgkqlln sdalsrlpqq ttrdggegpt ewshdwtrpp
       61 dsslyvrnyg lgekwipahv ksatgarmvt vepptaivkr qvdqvrrrsd ssprfpvtdt
      121 targpgpsqt kgpctaanvt tpleaaapdd spdtaqiagn niqprtsllp ptlspaeasq
//
LOCUS       XP_077497488             282 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108078 [Amblyomma americanum].
ACCESSION   XP_077497488
VERSION     XP_077497488.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641362.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..282
                     /product="uncharacterized protein LOC144108078"
                     /calculated_mol_wt=30162
     Region          <78..139
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..282
                     /gene="LOC144108078"
                     /coded_by="XM_077641362.1:271..1119"
                     /db_xref="GeneID:144108078"
ORIGIN      
        1 mvgpfergtg vpddlhglnf kpasygsvpt qdpkaprggd gptcdavtvv ehcpcgdhrg
       61 vddmhkhsdd atgtaaagre ftlvtddqpl vsplrpdrqt ptmtaariqr walylggyny
      121 klqyvpgkql lnsdalsrlt qqttrdrgeg ptegshdwtr ppdsslyvrn ygqgekwipg
      181 hvksatgarm vtvepptaiv krqvdqvrrr sdssprfpvt dttargtgps qtkgpctaan
      241 vttpleaaap dyspdtaqia gnniqprtsl lpptlspaea sq
//
LOCUS       XP_077497498             346 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108088 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077497498
VERSION     XP_077497498.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641372.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..346
                     /product="uncharacterized protein LOC144108088 isoform X1"
                     /calculated_mol_wt=37010
     Region          <142..203
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..346
                     /gene="LOC144108088"
                     /coded_by="XM_077641372.1:165..1205"
                     /db_xref="GeneID:144108088"
ORIGIN      
        1 mlprepgplr ggeevqpged saacaawrdr gdqpcscccc sgaclwrqqq npqtsggppv
       61 vhdnmvgpfe rgtgvpddlh glnfkpasyg svptqdpkap rcgdgptcda vtvvehcpcg
      121 dhrgvddmhk hsddatgtaa agreftlvtd dqplvsplrp drqtptmtaa riqrwalylg
      181 gynyklqyvp gkqllnsdal srltqqttrd rgegptegsh dwtrppdssl yvrnygqgek
      241 wipghvksat garmitvepp taivkrqvdq vrrrsdsspr fpvtdttarg pgpsqtkgpc
      301 taanvttple aaapdyspdt aqiagnniqp rtsllpptls paeasq
//
LOCUS       XP_077497506             346 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108088 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077497506
VERSION     XP_077497506.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641380.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..346
                     /product="uncharacterized protein LOC144108088 isoform X2"
                     /calculated_mol_wt=37010
     Region          <142..203
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..346
                     /gene="LOC144108088"
                     /coded_by="XM_077641380.1:169..1209"
                     /db_xref="GeneID:144108088"
ORIGIN      
        1 mlprepgplr ggeevqpged saacaawrdr gdqpcscccc sgaclwrqqq npqtsggppv
       61 vhdnmvgpfe rgtgvpddlh glnfkpasyg svptqdpkap rcgdgptcda vtvvehcpcg
      121 dhrgvddmhk hsddatgtaa agreftlvtd dqplvsplrp drqtptmtaa riqrwalylg
      181 gynyklqyvp gkqllnsdal srltqqttrd rgegptegsh dwtrppdssl yvrnygqgek
      241 wipghvksat garmitvepp taivkrqvdq vrrrsdsspr fpvtdttarg pgpsqtkgpc
      301 taanvttple aaapdyspdt aqiagnniqp rtsllpptls paeasq
//
LOCUS       XP_077497516             180 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108106 [Amblyomma americanum].
ACCESSION   XP_077497516
VERSION     XP_077497516.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641390.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..180
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..180
                     /product="uncharacterized protein LOC144108106"
                     /calculated_mol_wt=19427
     Region          <4..37
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..180
                     /gene="LOC144108106"
                     /coded_by="XM_077641390.1:87..629"
                     /db_xref="GeneID:144108106"
ORIGIN      
        1 mtaariqrwa lylggynykl qyvpgkqlln sdalsrltqq ttrdrgegpt egshdwtrpp
       61 dsslyvrnyg qgekwipghv ksatgarmvt vepptaivkr qvdqvrrrsd ssprfpvtdt
      121 targpgpsqt kgpctaanvt tpleaaapdy spdtaqiagn niqprtsllp ptlspaeasq
//
LOCUS       XP_077497526             219 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108117 [Amblyomma americanum].
ACCESSION   XP_077497526
VERSION     XP_077497526.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641400.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..219
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..219
                     /product="uncharacterized protein LOC144108117"
                     /calculated_mol_wt=23630
     Region          <15..76
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..219
                     /gene="LOC144108117"
                     /coded_by="XM_077641400.1:4..663"
                     /db_xref="GeneID:144108117"
ORIGIN      
        1 mhkhgddatg taaagreftl vtddqplvsp lrpdrqtptm taariqrwal ylggynyklq
       61 yvtgkqllns dalsrltqqt trdrgegpte gshdwtrppd sslyvrnygq gekwipghvk
      121 satgarmvtv epptaivkrq vdqvrrrsds sprfpvtdtt argpgpsqtk gpctaanvtt
      181 pleaaapeys pdtaqiagnn iqprtsllpp tlspaeasq
//
LOCUS       XP_077497536             219 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108128 [Amblyomma americanum].
ACCESSION   XP_077497536
VERSION     XP_077497536.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641410.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..219
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..219
                     /product="uncharacterized protein LOC144108128"
                     /calculated_mol_wt=23626
     Region          <15..76
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..219
                     /gene="LOC144108128"
                     /coded_by="XM_077641410.1:4..663"
                     /db_xref="GeneID:144108128"
ORIGIN      
        1 mhkhgddatg taaagreftl vtddqplvsp lrpdrqtptm taariqrwal ylggynyklq
       61 yvpgkqllns dalsrltqqt trdrgegpte gshdwtrppd sslyvrnygq gekwipghvk
      121 satgarmitv epptaivkrq vdqvrrrsds sprfpvtdtt argpgpsqtk gpctaanvtt
      181 pleaaapdys pdtaqiagnn iqprtsllpp tlspaeasq
//
LOCUS       XP_077497546             346 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108139 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077497546
VERSION     XP_077497546.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641420.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..346
                     /product="uncharacterized protein LOC144108139 isoform X1"
                     /calculated_mol_wt=36964
     Region          <142..203
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..346
                     /gene="LOC144108139"
                     /coded_by="XM_077641420.1:257..1297"
                     /db_xref="GeneID:144108139"
ORIGIN      
        1 mlprepgplr ggeevqpged saacaawrdr gdqpcscccc sgaclwrqqq npqtsggppv
       61 vhdnmvgpfe rgtgvpddlh glnfkpasyg svptqdpkap rggdgptcda vtvvehcpcg
      121 dhrgvddmhk hsddatgtaa agreftlvtd dqplvsplrp drqtptmtaa riqrwalylg
      181 gynyklqyvp gkqllnsdal srltqqttrd rgegptegsh dwtrppdssl yvrnygqgek
      241 wipghvksat garmvtvepp taivkrqvdq vrrrsdsspr fpvtdttarg pgpsqtkgpc
      301 taanvttple aaapeyspdt aqiagnniqp rtsllpptls paeasq
//
LOCUS       XP_077497553             346 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108139 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077497553
VERSION     XP_077497553.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641427.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..346
                     /product="uncharacterized protein LOC144108139 isoform X2"
                     /calculated_mol_wt=36964
     Region          <142..203
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..346
                     /gene="LOC144108139"
                     /coded_by="XM_077641427.1:261..1301"
                     /db_xref="GeneID:144108139"
ORIGIN      
        1 mlprepgplr ggeevqpged saacaawrdr gdqpcscccc sgaclwrqqq npqtsggppv
       61 vhdnmvgpfe rgtgvpddlh glnfkpasyg svptqdpkap rggdgptcda vtvvehcpcg
      121 dhrgvddmhk hsddatgtaa agreftlvtd dqplvsplrp drqtptmtaa riqrwalylg
      181 gynyklqyvp gkqllnsdal srltqqttrd rgegptegsh dwtrppdssl yvrnygqgek
      241 wipghvksat garmvtvepp taivkrqvdq vrrrsdsspr fpvtdttarg pgpsqtkgpc
      301 taanvttple aaapeyspdt aqiagnniqp rtsllpptls paeasq
//
LOCUS       XP_077497564             346 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108157 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077497564
VERSION     XP_077497564.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641438.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..346
                     /product="uncharacterized protein LOC144108157 isoform X1"
                     /calculated_mol_wt=37005
     Region          <142..203
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..346
                     /gene="LOC144108157"
                     /coded_by="XM_077641438.1:378..1418"
                     /db_xref="GeneID:144108157"
ORIGIN      
        1 mlprepgplr ggeevqpged saacaawrdr gdqpcscccc sgaclwrqqq npqtsggppv
       61 vhdnmvgpfe rgtgvpddlh glnfkpasyg svptqdpkap rggdgptcda vtvvehcpcg
      121 dhrgvddmhk hsddatgtaa agreftlvtd dqplvsplrp drqtptmtaa riqrwalylg
      181 gynyklqyvp gkqllnsdal srltqqttrd rgegptegsh dwtrppdssl yvrnygqgek
      241 wipghvksat garmvtvepp taivkrqvdq vrrrsdsspr fpvtdttarg pgpsqtkgpc
      301 taanvttple aaapdyspdr aqiagnniqp rtsllpptls paeasq
//
LOCUS       XP_077497572             346 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108157 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077497572
VERSION     XP_077497572.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641446.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..346
                     /product="uncharacterized protein LOC144108157 isoform X2"
                     /calculated_mol_wt=37005
     Region          <142..203
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..346
                     /gene="LOC144108157"
                     /coded_by="XM_077641446.1:382..1422"
                     /db_xref="GeneID:144108157"
ORIGIN      
        1 mlprepgplr ggeevqpged saacaawrdr gdqpcscccc sgaclwrqqq npqtsggppv
       61 vhdnmvgpfe rgtgvpddlh glnfkpasyg svptqdpkap rggdgptcda vtvvehcpcg
      121 dhrgvddmhk hsddatgtaa agreftlvtd dqplvsplrp drqtptmtaa riqrwalylg
      181 gynyklqyvp gkqllnsdal srltqqttrd rgegptegsh dwtrppdssl yvrnygqgek
      241 wipghvksat garmvtvepp taivkrqvdq vrrrsdsspr fpvtdttarg pgpsqtkgpc
      301 taanvttple aaapdyspdr aqiagnniqp rtsllpptls paeasq
//
LOCUS       XP_077497582             180 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108175 [Amblyomma americanum].
ACCESSION   XP_077497582
VERSION     XP_077497582.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641456.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..180
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..180
                     /product="uncharacterized protein LOC144108175"
                     /calculated_mol_wt=19427
     Region          <4..37
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..180
                     /gene="LOC144108175"
                     /coded_by="XM_077641456.1:101..643"
                     /db_xref="GeneID:144108175"
ORIGIN      
        1 mtaariqrwa lylggynykl qyvpgkqlln sdalsrltqq ttrdrgegpt egshdwtrpp
       61 dsslyvrnyg qgekwipghv ksatgarmvt vepptaivkr qvdqvrrrsd ssprfpvtdt
      121 targpgpsqt kgpctaanvt tpleaaapdy spdtaqiagn niqprtsllp ptlspaeasq
//
LOCUS       XP_077497592             346 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108185 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077497592
VERSION     XP_077497592.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641466.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..346
                     /product="uncharacterized protein LOC144108185 isoform X1"
                     /calculated_mol_wt=36991
     Region          <142..203
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..346
                     /gene="LOC144108185"
                     /coded_by="XM_077641466.1:179..1219"
                     /db_xref="GeneID:144108185"
ORIGIN      
        1 mlprepgplr ggeevqpged saacaawrdr gdqpcscccc sgaclwrqqq npqtsggppv
       61 vhdnmvgpfe rgtgvpddlh glnfkpasyg svptqdpkap rggdgptcda vtvvehcpcg
      121 dhrgvddmhk hsddatgtaa agreftlvtd dqplvsplrp drqtptmtaa riqrwalylg
      181 gynyklqyvt gkqllhsdal srltqqttrd rgegptegsh dwtrppdssl yvrnygqgek
      241 wipghvksat garmvtvepp taivkrqvdq vrrrsdsspr fpvtdttarg pgpsqtkgpc
      301 taanvttple aaapeyspdt aqiagnniqp rtsllpptls paeasq
//
LOCUS       XP_077497600             346 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108185 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077497600
VERSION     XP_077497600.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641474.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..346
                     /product="uncharacterized protein LOC144108185 isoform X2"
                     /calculated_mol_wt=36991
     Region          <142..203
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..346
                     /gene="LOC144108185"
                     /coded_by="XM_077641474.1:183..1223"
                     /db_xref="GeneID:144108185"
ORIGIN      
        1 mlprepgplr ggeevqpged saacaawrdr gdqpcscccc sgaclwrqqq npqtsggppv
       61 vhdnmvgpfe rgtgvpddlh glnfkpasyg svptqdpkap rggdgptcda vtvvehcpcg
      121 dhrgvddmhk hsddatgtaa agreftlvtd dqplvsplrp drqtptmtaa riqrwalylg
      181 gynyklqyvt gkqllhsdal srltqqttrd rgegptegsh dwtrppdssl yvrnygqgek
      241 wipghvksat garmvtvepp taivkrqvdq vrrrsdsspr fpvtdttarg pgpsqtkgpc
      301 taanvttple aaapeyspdt aqiagnniqp rtsllpptls paeasq
//
LOCUS       XP_077497610             346 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108202 [Amblyomma americanum].
ACCESSION   XP_077497610
VERSION     XP_077497610.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641484.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..346
                     /product="uncharacterized protein LOC144108202"
                     /calculated_mol_wt=36976
     Region          <142..203
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..346
                     /gene="LOC144108202"
                     /coded_by="XM_077641484.1:49..1089"
                     /db_xref="GeneID:144108202"
ORIGIN      
        1 mlprepgplr ggeevqpged saacaawrdr gdqpcscccc sgaclwrqqq npqtsggppv
       61 vhdnmvgpfe rgtgvpddlh glnfkpasyg svptqdpkap rggdgptcda vtvvehcpcg
      121 dhrgvddmhk hsddatgtaa agreftlvtd dqplvsplrp drqtptmtaa riqrwalylg
      181 gynyklqyvp gkqllnsdal srltqqttrd rgegptegsy dwtrppdssl yvrnygqgek
      241 wipghvksat garmvtvepp taivkrqvdq vrrrsdsspr fpvtdttarg pgpsqtkgpc
      301 taanvttple aaapdyspdt aqiagnniqp rtsllpptls paeasq
//
LOCUS       XP_077497621             346 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108213 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077497621
VERSION     XP_077497621.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641495.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..346
                     /product="uncharacterized protein LOC144108213 isoform X1"
                     /calculated_mol_wt=37005
     Region          <142..203
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..346
                     /gene="LOC144108213"
                     /coded_by="XM_077641495.1:331..1371"
                     /db_xref="GeneID:144108213"
ORIGIN      
        1 mlprepgplr ggeevqpged saacaawrdr gdqpcscccc sgaclwrqqq npqtsggppv
       61 vhdnmvgpfe rgtgvpddlh glnfkpasyg svptqdpkap rggdgptcda vtvvehcpcg
      121 dhrgvddmhk hsddatgtaa agreftlvtd dqplvsplrp drqtptmtaa riqrwalylg
      181 gynyklqyvp gkqllnsdal srltqqttrd rgegptegsh dwtrppdssl yvrnygqgek
      241 wipghvksat garmvtvepp taivkrqvdq vrrrsdsspr fpvtdttarg pgpsqtkgpc
      301 taanvttple aaapdyspdr aqiagnniqp rtsllpptls paeasq
//
LOCUS       XP_077497626             346 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108213 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077497626
VERSION     XP_077497626.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641500.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..346
                     /product="uncharacterized protein LOC144108213 isoform X2"
                     /calculated_mol_wt=37005
     Region          <142..203
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..346
                     /gene="LOC144108213"
                     /coded_by="XM_077641500.1:335..1375"
                     /db_xref="GeneID:144108213"
ORIGIN      
        1 mlprepgplr ggeevqpged saacaawrdr gdqpcscccc sgaclwrqqq npqtsggppv
       61 vhdnmvgpfe rgtgvpddlh glnfkpasyg svptqdpkap rggdgptcda vtvvehcpcg
      121 dhrgvddmhk hsddatgtaa agreftlvtd dqplvsplrp drqtptmtaa riqrwalylg
      181 gynyklqyvp gkqllnsdal srltqqttrd rgegptegsh dwtrppdssl yvrnygqgek
      241 wipghvksat garmvtvepp taivkrqvdq vrrrsdsspr fpvtdttarg pgpsqtkgpc
      301 taanvttple aaapdyspdr aqiagnniqp rtsllpptls paeasq
//
LOCUS       XP_077497636             282 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108229 [Amblyomma americanum].
ACCESSION   XP_077497636
VERSION     XP_077497636.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641510.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..282
                     /product="uncharacterized protein LOC144108229"
                     /calculated_mol_wt=30159
     Region          <78..139
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..282
                     /gene="LOC144108229"
                     /coded_by="XM_077641510.1:128..976"
                     /db_xref="GeneID:144108229"
ORIGIN      
        1 mvgpfergtg vpddlhglnf kpasygsvpt qdpkaprggd gptcdavtvv ehcpcgdhrg
       61 vddmhkhsdd atgtaaagre ftlvtddqpl vsplrpdrqt ptmtaariqr walylggyny
      121 klqyvpgkql lnsdalsrlt qqttrdrgeg ptegshdwtr ppdsslyvrn ygqgekwipg
      181 hvksatgarm vtvepptaiv krqvdqvrrr sdssprfpvt dttargpgps qtkgpctaan
      241 vttpleaaap dyspdtaeia gnniqprtsl lpptlspaea sq
//
LOCUS       XP_077497674             146 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108275 [Amblyomma americanum].
ACCESSION   XP_077497674
VERSION     XP_077497674.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641548.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..146
                     /product="uncharacterized protein LOC144108275"
                     /calculated_mol_wt=15851
     CDS             1..146
                     /gene="LOC144108275"
                     /coded_by="XM_077641548.1:269..709"
                     /db_xref="GeneID:144108275"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdpthrppfa altsvlccqr pasigpcvlp afgcvippdp
       61 fmlktkscme vvqatklrvi fardleqagk lmdgqwysls kasffsktkt iflgswamyl
      121 gtcgqdnagq hasasskkkh ktvnky
//
LOCUS       XP_077497707             183 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108314 [Amblyomma americanum].
ACCESSION   XP_077497707
VERSION     XP_077497707.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641581.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="uncharacterized protein LOC144108314"
                     /calculated_mol_wt=21466
     CDS             1..183
                     /gene="LOC144108314"
                     /coded_by="XM_077641581.1:266..817"
                     /db_xref="GeneID:144108314"
ORIGIN      
        1 mswdahvskv clslsryigi isrnkhilpa ktklnlyyaf fysyvsycfl vwgqttasni
       61 qkllvlqkri lrvipnvpyd aptrpiyqqy rmipvtrmfd yrfssfykki iaeddaffsg
      121 ivtltphrtp yvtrqrmiys lprcrtnygf stlkyilpky lntpfydtlh smsvsairnv
      181 fss
//
LOCUS       XP_077497723             405 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108335 [Amblyomma americanum].
ACCESSION   XP_077497723
VERSION     XP_077497723.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641597.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..405
                     /product="uncharacterized protein LOC144108335"
                     /calculated_mol_wt=45288
     CDS             1..405
                     /gene="LOC144108335"
                     /coded_by="XM_077641597.1:1..1218"
                     /db_xref="GeneID:144108335"
ORIGIN      
        1 mffpsptgpn ikppmltasf ktlrelhske qgqliksapt lsskslnpsn ierqnvelal
       61 rilspstaaa lracgprlel dhvsataqfl etttkwwniv nvktcnkgtr lrdelqspit
      121 sassphiqfl lsivewldlw qslkfdtgil trethsalrl ttdtlvkvtr ycldemnfey
      181 vllgkfqtdc learfgkyrq lsgaqyhisi rqvyeserkl rlqnilelpe feaatdavav
      241 ndavlevfdi evtdddyaka pnlpattyva gycahaafkk lscmtckenl mledeieveg
      301 gelvkamtrg ilkfpqpaiv navltaeivl dklrseqhat qfhalsnqke dllalthnvl
      361 ndgidfdvce nghtpqlvmh yvlsaaantl lnnlckrknd qlive
//
LOCUS       XP_077497784             266 aa            linear   INV 25-NOV-2025
DEFINITION  staphylococcal nuclease domain-containing protein 1-like [Amblyomma
            americanum].
ACCESSION   XP_077497784
VERSION     XP_077497784.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641658.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..266
                     /product="staphylococcal nuclease domain-containing
                     protein 1-like"
                     /calculated_mol_wt=29112
     Region          12..109
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     CDS             1..266
                     /gene="LOC144108413"
                     /coded_by="XM_077641658.1:110..910"
                     /db_xref="GeneID:144108413"
ORIGIN      
        1 mvlvwrtpdg lfvqqqsltg elkdmmealn awvkshspep sttkyskgdy acalfsedsi
       61 wyrgqvlseq spdgtylvlf vdfgnrerml asslrplpls faekplfvfc vvpqnvpten
      121 srlaallqqe pftavqvett raglpvvrle ckdgtcindl ishaphkdpa qsaatgsapq
      181 eepqkppvav qtlvdvpdcp lpkglfdavv asvegnlvyt qpiirapgll kvtealvnka
      241 rrdpgykfte mppvnqcvla lysdeq
//
LOCUS       XP_077497811             135 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108450 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077497811
VERSION     XP_077497811.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641685.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..135
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..135
                     /product="uncharacterized protein LOC144108450 isoform X1"
                     /calculated_mol_wt=15468
     CDS             1..135
                     /gene="LOC144108450"
                     /coded_by="XM_077641685.1:434..841"
                     /db_xref="GeneID:144108450"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedrrr rhlnrrltat mkrlsgvhvl
       61 nhehrfldrg idqmskiiva alvkiygpgi asasrarpge vyvvhrcrrc gakghktdhc
      121 waycsprrha aagrg
//
LOCUS       XP_077497815             135 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108450 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077497815
VERSION     XP_077497815.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641689.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..135
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..135
                     /product="uncharacterized protein LOC144108450 isoform X1"
                     /calculated_mol_wt=15468
     CDS             1..135
                     /gene="LOC144108450"
                     /coded_by="XM_077641689.1:372..779"
                     /db_xref="GeneID:144108450"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedrrr rhlnrrltat mkrlsgvhvl
       61 nhehrfldrg idqmskiiva alvkiygpgi asasrarpge vyvvhrcrrc gakghktdhc
      121 waycsprrha aagrg
//
LOCUS       XP_077497818              85 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108450 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077497818
VERSION     XP_077497818.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641692.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..85
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..85
                     /product="uncharacterized protein LOC144108450 isoform X2"
                     /calculated_mol_wt=9314
     CDS             1..85
                     /gene="LOC144108450"
                     /coded_by="XM_077641692.1:513..770"
                     /db_xref="GeneID:144108450"
ORIGIN      
        1 mkrlsgvhvl nhehrfldrg idqmskiiva alvkiygpgi asasrarpge vyvvhrcrrc
       61 gakghktdhc waycsprrha aagrg
//
LOCUS       XP_077497821              85 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108450 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077497821
VERSION     XP_077497821.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641695.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..85
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..85
                     /product="uncharacterized protein LOC144108450 isoform X2"
                     /calculated_mol_wt=9314
     CDS             1..85
                     /gene="LOC144108450"
                     /coded_by="XM_077641695.1:451..708"
                     /db_xref="GeneID:144108450"
ORIGIN      
        1 mkrlsgvhvl nhehrfldrg idqmskiiva alvkiygpgi asasrarpge vyvvhrcrrc
       61 gakghktdhc waycsprrha aagrg
//
LOCUS       XP_077497827              85 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108450 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077497827
VERSION     XP_077497827.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641701.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..85
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..85
                     /product="uncharacterized protein LOC144108450 isoform X2"
                     /calculated_mol_wt=9314
     CDS             1..85
                     /gene="LOC144108450"
                     /coded_by="XM_077641701.1:430..687"
                     /db_xref="GeneID:144108450"
ORIGIN      
        1 mkrlsgvhvl nhehrfldrg idqmskiiva alvkiygpgi asasrarpge vyvvhrcrrc
       61 gakghktdhc waycsprrha aagrg
//
LOCUS       XP_077497858             497 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108513 [Amblyomma americanum].
ACCESSION   XP_077497858
VERSION     XP_077497858.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641732.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..497
                     /product="uncharacterized protein LOC144108513"
                     /calculated_mol_wt=53691
     CDS             1..497
                     /gene="LOC144108513"
                     /coded_by="XM_077641732.1:16..1509"
                     /db_xref="GeneID:144108513"
ORIGIN      
        1 mpttvlafas lllpslagls tgassvcalp ytgddpgsch lppdecssll nvppeelhtp
       61 eiivpwvats tsatmptcik elhpltplrg lgdcttaqpp npltfamqtt vlafaslllp
      121 slaglstgas svcalpytgd dpgschlppd ecsslldvpp eelhtpeitv pcvatstsat
      181 mptcikelhp ltplrglgdc ttaqppnplt famqvstsya lfakkssnyi lvqfpslhcc
      241 vaiavecahv ihsllmlsgd fetnpgpggn aavlaekqkl nagqtllitk iqglktqlnt
      301 nnqtiaslyk rmadleahyq tllhlrndie imqsttinqa kkteeletrl ddaenqsrrn
      361 nlifygildp asaetwaese klvidvcrnn lditlepndi eranrlgnhs adrtrpviak
      421 flshktkdal lsngrklkdt nysigedfsr tvrharkqll vfakansnky slrfktlhig
      481 skryvfdass qtvkeia
//
LOCUS       XP_077497882              87 aa            linear   INV 25-NOV-2025
DEFINITION  THAP domain-containing protein 2-like [Amblyomma americanum].
ACCESSION   XP_077497882
VERSION     XP_077497882.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641756.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..87
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..87
                     /product="THAP domain-containing protein 2-like"
                     /calculated_mol_wt=9924
     Region          4..80
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..87
                     /gene="LOC144108542"
                     /coded_by="XM_077641756.1:33..296"
                     /db_xref="GeneID:144108542"
ORIGIN      
        1 mpsccvpgcr srtpgqepgv tfhtfpkdpe rrrqwteavr pgqrdwepak wtcvcskhfl
       61 pehfdrmspl vvriradavp lqqepls
//
LOCUS       XP_077497898             398 aa            linear   INV 25-NOV-2025
DEFINITION  octopamine receptor beta-2R-like [Amblyomma americanum].
ACCESSION   XP_077497898
VERSION     XP_077497898.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641772.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..398
                     /product="octopamine receptor beta-2R-like"
                     /calculated_mol_wt=44666
     Region          32..352
                     /region_name="7tmA_DmOct-betaAR-like"
                     /note="Drosophila melanogaster beta-adrenergic
                     receptor-like octopamine receptors and similar receptors
                     in bilateria; member of the class A family of
                     seven-transmembrane G protein-coupled receptors; cd15066"
                     /db_xref="CDD:320194"
     Region          32..58
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320194"
     Region          65..91
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320194"
     Region          103..133
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320194"
     Site            order(106,110..111,114..115,189,196,199..200,203,299,
                     302..303,306,323,327,331)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:320194"
     Region          145..168
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320194"
     Region          192..221
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320194"
     Region          279..309
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320194"
     Region          320..345
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320194"
     CDS             1..398
                     /gene="LOC144108568"
                     /coded_by="XM_077641772.1:24..1220"
                     /db_xref="GeneID:144108568"
ORIGIN      
        1 mtvaapnasl gnltdeeagd deswegvlkl vlktlalvti issavfgnll vvtsvirhhk
       61 lrittnyfiv slaladtlva lfamtfnasv tisgrwlfnq tvcdfwnscd vlfstasimh
      121 lccisvdryy aiikpleypt kitgrtvaim lacawissgl isfipifmgw yttdehlgyk
      181 ldhpdecifv vnkpyaivss svsfwipcci mlftywriyv eatrqekmlc ksqmgpagml
      241 crnstdlpnv happhrnshg ddpesgqstp tkrtinkmkr ehkaaktlgi imgafilcwl
      301 pfflwyvsvt mcgdacpcpd lvvdllfwig ylnsslnpvi yayfnrefrq afketlqaif
      361 csckgascfm trsrsrairf dctyrstldv aeskclrd
//
LOCUS       XP_077497911             340 aa            linear   INV 25-NOV-2025
DEFINITION  prolactin-releasing peptide receptor-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077497911
VERSION     XP_077497911.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641785.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..340
                     /product="prolactin-releasing peptide receptor-like
                     isoform X1"
                     /calculated_mol_wt=36853
     Region          30..299
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          31..55
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320331"
     Region          64..86
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320331"
     Region          102..124
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320331"
     Region          146..162
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320331"
     Region          190..213
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320331"
     Region          230..252
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320331"
     Region          267..292
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320331"
     CDS             1..340
                     /gene="LOC144108584"
                     /coded_by="XM_077641785.1:75..1097"
                     /db_xref="GeneID:144108584"
ORIGIN      
        1 mddqlgaths iadrngtapa ggggvaaral fgllyaivfv lgvsgnglvc avvlrrramr
       61 tvtnllvanl alsdillcal avpftplylf lrrwpfgaal crlvpfaqgv svyvssltlt
      121 aiavhrlrav vhplrrrllr prscavlcaa lwaasalltl pyaafvrlvv saadgrafce
      181 elwpsehgrq lfgaltaavq fllplavvgg cyarvarrlr rrsrrarrtq lmllamvlaf
      241 alawlplnvc nlladfhaas vawplgdalf caahaaamss tcynpllyaw fndnfrrqfv
      301 rppsaaeqtv letlrsdrph drweppppaa drtnappall
//
LOCUS       XP_077497915             333 aa            linear   INV 25-NOV-2025
DEFINITION  prolactin-releasing peptide receptor-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077497915
VERSION     XP_077497915.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641789.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..333
                     /product="prolactin-releasing peptide receptor-like
                     isoform X2"
                     /calculated_mol_wt=37028
     Region          17..298
                     /region_name="7tmA_NPYR-like"
                     /note="neuropeptide Y receptors and related proteins,
                     member of the class A family of seven-transmembrane G
                     protein-coupled receptors; cd15203"
                     /db_xref="CDD:320331"
     Region          18..44
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320331"
     Region          51..76
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320331"
     Site            order(73,76..77,89..94,96..97,100,143,145..149,172,
                     175..177,179..181,183..184,243,246..247,249..250,253,
                     265..266,268..270,273,276..277)
                     /site_type="other"
                     /note="putative peptide ligand binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:320331"
     Region          89..119
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320331"
     Region          130..151
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320331"
     Region          172..201
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320331"
     Region          225..253
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320331"
     Region          266..291
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320331"
     CDS             1..333
                     /gene="LOC144108584"
                     /coded_by="XM_077641789.1:304..1305"
                     /db_xref="GeneID:144108584"
ORIGIN      
        1 manatldyvs gvpavraffy cvyalvfavg vsgnalvcfv varqrsmhtv tnlfianlal
       61 sdillcalav pftplhqfvg awplgaalcr lvpfaqgvsv yvssftltai avdrffvimh
      121 pfrsrlrlpl cgalialiwl agalltlpyg lfmgltadga fceerwpser srrafslcts
      181 slqfglpfav vsfcylrvcc klrerarakp gakslqreqf errrtrrtnr mlvsmvvifg
      241 acwlplnlyn lavdfsvpaa rwqfanafff lahavamsst cynpflytwl ndsfrkefka
      301 vlpcfarpae ppppppvryv cssdavklpe qql
//
LOCUS       XP_077497921             321 aa            linear   INV 25-NOV-2025
DEFINITION  RCC1 domain-containing protein 1-like [Amblyomma americanum].
ACCESSION   XP_077497921
VERSION     XP_077497921.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641795.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..321
                     /product="RCC1 domain-containing protein 1-like"
                     /calculated_mol_wt=33298
     Region          <148..316
                     /region_name="ATS1"
                     /note="Alpha-tubulin suppressor ATS1 and related RCC1
                     domain-containing proteins [Cell cycle control, cell
                     division, chromosome partitioning, Cytoskeleton]; COG5184"
                     /db_xref="CDD:444065"
     CDS             1..321
                     /gene="LOC144108596"
                     /coded_by="XM_077641795.1:42..1007"
                     /db_xref="GeneID:144108596"
ORIGIN      
        1 msycyygfdk fecfcfvpgk cfrscpeaps plrsvhvgwa sailvteaga aelrgwggpr
       61 rlaaagvsqa algprghwll lleaggawlg rpgaprrwll derlcdaavg ssralllqqq
      121 qtggggggcv lelrldglav arpvaravrq vscghghqla ltadggalsw gdggrgqlgh
      181 ggvsteeapr piealaglql tavaaggwhs aalahggdly lwgwnrdgql ggspcelpla
      241 anprllpled vvsvalgsrh tvallgdgsa wawgsndygq vgphetgpsw ravrlaegra
      301 vgvscspwat lvqldsprdp f
//
LOCUS       XP_077497933             202 aa            linear   INV 25-NOV-2025
DEFINITION  protease-associated domain-containing protein 1 [Amblyomma
            americanum].
ACCESSION   XP_077497933
VERSION     XP_077497933.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641807.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..202
                     /product="protease-associated domain-containing protein 1"
                     /calculated_mol_wt=22341
     Region          65..182
                     /region_name="PA"
                     /note="Protease-associated (PA) domain. The PA domain is
                     an insert domain in a diverse fraction of proteases. The
                     significance of the PA domain to many of the proteins in
                     which it is inserted is undetermined. It may be a
                     protein-protein interaction domain. At...; cl28883"
                     /db_xref="CDD:333703"
     Site            140..142
                     /site_type="other"
                     /note="PA/protease or protease-like domain interface
                     [polypeptide binding]"
                     /db_xref="CDD:238300"
     CDS             1..202
                     /gene="LOC144108612"
                     /coded_by="XM_077641807.1:204..812"
                     /db_xref="GeneID:144108612"
ORIGIN      
        1 mrkrralvrg lshtasavll ayccvlvdla nlvsadgkfl kediffeile peslrytfrl
       61 rpahefgasf vsqlsnvglv lseplqgcas lvnrlelrhn vvlverggcs fltkcvevrr
      121 qgalavivad sdpgnddqyv dmmddstrrn csipaafllg kdgymirrgl eahglrrali
      181 nipvnvsgvp ahrlrqppwl vw
//
LOCUS       XP_077497940             272 aa            linear   INV 25-NOV-2025
DEFINITION  prohibitin 1 [Amblyomma americanum].
ACCESSION   XP_077497940
VERSION     XP_077497940.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641814.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..272
                     /product="prohibitin 1"
                     /calculated_mol_wt=29848
     Region          27..221
                     /region_name="SPFH_prohibitin"
                     /note="Prohibitin family; SPFH (stomatin, prohibitin,
                     flotillin, and HflK/C) superfamily; cd03401"
                     /db_xref="CDD:259799"
     Region          <190..262
                     /region_name="PRK11029"
                     /note="protease modulator HflC"
                     /db_xref="CDD:182913"
     CDS             1..272
                     /gene="Phb1"
                     /coded_by="XM_077641814.1:146..964"
                     /db_xref="GeneID:144108622"
ORIGIN      
        1 maaqifntlg klgiglavvg gvansalynv dgghravifd rftgvknyvv gegthflipw
       61 vqrpiiydvr srprnvpvvt gskdlqnvni tlrilfrpvq eqlprmyttl gvdydervlp
      121 sitnevlkav vaqfdasemi tqrevvsqkv cdelterasq fgvilddisi thltfgkeft
      181 qavemkqvaq qeaerarflv ekaeqhkkaa vitaegdsqa aallakafge agdalvelrr
      241 leaaedisfq lsrarnvvyl ppgqstllsl pq
//
LOCUS       XP_077497947             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108632 [Amblyomma americanum].
ACCESSION   XP_077497947
VERSION     XP_077497947.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641821.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144108632"
                     /calculated_mol_wt=14258
     CDS             1..133
                     /gene="LOC144108632"
                     /coded_by="XM_077641821.1:1754..2155"
                     /db_xref="GeneID:144108632"
ORIGIN      
        1 mpfsyqtvtd ldndtsetls apgcpglarv hgdsalaeas mdfiidrsst qavlsapgpy
       61 dlgaalegle lseapmditl hnsallvcpw plgpsrhffv anphlhrltp lrrrscelvq
      121 qdlllsnrgh gsr
//
LOCUS       XP_077497955             648 aa            linear   INV 25-NOV-2025
DEFINITION  protein SERAC1-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077497955
VERSION     XP_077497955.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641829.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..648
                     /product="protein SERAC1-like isoform X1"
                     /calculated_mol_wt=72508
     Region          <412..502
                     /region_name="EstA"
                     /note="Triacylglycerol esterase/lipase EstA, alpha/beta
                     hydrolase fold [Lipid transport and metabolism]; COG1075"
                     /db_xref="CDD:440693"
     CDS             1..648
                     /gene="LOC144108642"
                     /coded_by="XM_077641829.1:133..2079"
                     /db_xref="GeneID:144108642"
ORIGIN      
        1 mmklrngvvi gcitaalgsg yalrhdvgfm shlygerpsa alnespyicf sgksapatea
       61 spswslkhif rkearphwhl lsearskvqr vrheavkalg smtslsdsel ceiaqacdcq
      121 tsvglartag adlrcflspp pipdyiakds lqdcmrmvls rlpetapesc itfftknalq
      181 tklqyfdahh ekedslrwrr pavgqaellc lqallshtal pehrrllvek lavlpllqrv
      241 lhehthdval hslvaqmlan laldpalhep ifragwvgil arwlrspevl lsfpagrala
      301 nmdtdalspt eywdgvyllh pqyrnrepsv tadiifvhgl lgsvfktwrq hdsqgtlnkl
      361 eslleeptvs avrssackim vdhtsprara mhqkytkcwp kswlaedipn trilaldydt
      421 ylsqwspncp lesdkrtlvf ratdmlaklh ragvggrpii withsmggll vkqmlavase
      481 spqyeamvrn tagvvfyavp hrgadlgdfs asfkllllps vevqelgkda phllevhekf
      541 eklvkmfnip cisfgesakt ilgfrmskvl vpvesadpgf gdffvvntsh leickphrws
      601 sfsyqllldf ldhafssisq daywaahnpt lsrlrqlely fetgvfqf
//
LOCUS       XP_077497959             647 aa            linear   INV 25-NOV-2025
DEFINITION  protein SERAC1-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077497959
VERSION     XP_077497959.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641833.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..647
                     /product="protein SERAC1-like isoform X2"
                     /calculated_mol_wt=72452
     Region          <411..501
                     /region_name="EstA"
                     /note="Triacylglycerol esterase/lipase EstA, alpha/beta
                     hydrolase fold [Lipid transport and metabolism]; COG1075"
                     /db_xref="CDD:440693"
     CDS             1..647
                     /gene="LOC144108642"
                     /coded_by="XM_077641833.1:320..2263"
                     /db_xref="GeneID:144108642"
ORIGIN      
        1 mmklrngvvi gcitaalgsg yalrhdvgfm shlygerpsa alnespyicf sesapateas
       61 pswslkhifr kearphwhll searskvqrv rheavkalgs mtslsdselc eiaqacdcqt
      121 svglartaga dlrcflsppp ipdyiakdsl qdcmrmvlsr lpetapesci tfftknalqt
      181 klqyfdahhe kedslrwrrp avgqaellcl qallshtalp ehrrllvekl avlpllqrvl
      241 hehthdvalh slvaqmlanl aldpalhepi fragwvgila rwlrspevll sfpagralan
      301 mdtdalspte ywdgvyllhp qyrnrepsvt adiifvhgll gsvfktwrqh dsqgtlnkle
      361 slleeptvsa vrssackimv dhtspraram hqkytkcwpk swlaedipnt rilaldydty
      421 lsqwspncpl esdkrtlvfr atdmlaklhr agvggrpiiw ithsmggllv kqmlavases
      481 pqyeamvrnt agvvfyavph rgadlgdfsa sfkllllpsv evqelgkdap hllevhekfe
      541 klvkmfnipc isfgesakti lgfrmskvlv pvesadpgfg dffvvntshl eickphrwss
      601 fsyqllldfl dhafssisqd aywaahnptl srlrqlelyf etgvfqf
//
LOCUS       XP_077497963             637 aa            linear   INV 25-NOV-2025
DEFINITION  protein SERAC1-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077497963
VERSION     XP_077497963.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641837.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..637
                     /product="protein SERAC1-like isoform X3"
                     /calculated_mol_wt=71223
     Region          <412..502
                     /region_name="EstA"
                     /note="Triacylglycerol esterase/lipase EstA, alpha/beta
                     hydrolase fold [Lipid transport and metabolism]; COG1075"
                     /db_xref="CDD:440693"
     CDS             1..637
                     /gene="LOC144108642"
                     /coded_by="XM_077641837.1:133..2046"
                     /db_xref="GeneID:144108642"
ORIGIN      
        1 mmklrngvvi gcitaalgsg yalrhdvgfm shlygerpsa alnespyicf sgksapatea
       61 spswslkhif rkearphwhl lsearskvqr vrheavkalg smtslsdsel ceiaqacdcq
      121 tsvglartag adlrcflspp pipdyiakds lqdcmrmvls rlpetapesc itfftknalq
      181 tklqyfdahh ekedslrwrr pavgqaellc lqallshtal pehrrllvek lavlpllqrv
      241 lhehthdval hslvaqmlan laldpalhep ifragwvgil arwlrspevl lsfpagrala
      301 nmdtdalspt eywdgvyllh pqyrnrepsv tadiifvhgl lgsvfktwrq hdsqgtlnkl
      361 eslleeptvs avrssackim vdhtsprara mhqkytkcwp kswlaedipn trilaldydt
      421 ylsqwspncp lesdkrtlvf ratdmlaklh ragvggrpii withsmggll vkqmlavase
      481 spqyeamvrn tagvvfyavp hrgadlgdfs asfkllllps vevqelgkda phllevhekf
      541 eklvkmfnip cisfgesakt ilgfrmskvl vpvesaaqvv aprrcrsrrr lrhltfrcsr
      601 allhmcvmcm rkeailcacq ridvrtrhgr egecgcc
//
LOCUS       XP_077497968             619 aa            linear   INV 25-NOV-2025
DEFINITION  protein SERAC1-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077497968
VERSION     XP_077497968.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641842.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..619
                     /product="protein SERAC1-like isoform X4"
                     /calculated_mol_wt=69475
     Region          <383..473
                     /region_name="EstA"
                     /note="Triacylglycerol esterase/lipase EstA, alpha/beta
                     hydrolase fold [Lipid transport and metabolism]; COG1075"
                     /db_xref="CDD:440693"
     CDS             1..619
                     /gene="LOC144108642"
                     /coded_by="XM_077641842.1:163..2022"
                     /db_xref="GeneID:144108642"
ORIGIN      
        1 mshlygerps aalnespyic fsgksapate aspswslkhi frkearphwh llsearskvq
       61 rvrheavkal gsmtslsdse lceiaqacdc qtsvglarta gadlrcflsp ppipdyiakd
      121 slqdcmrmvl srlpetapes citfftknal qtklqyfdah hekedslrwr rpavgqaell
      181 clqallshta lpehrrllve klavlpllqr vlhehthdva lhslvaqmla nlaldpalhe
      241 pifragwvgi larwlrspev llsfpagral anmdtdalsp teywdgvyll hpqyrnreps
      301 vtadiifvhg llgsvfktwr qhdsqgtlnk leslleeptv savrssacki mvdhtsprar
      361 amhqkytkcw pkswlaedip ntrilaldyd tylsqwspnc plesdkrtlv fratdmlakl
      421 hragvggrpi iwithsmggl lvkqmlavas espqyeamvr ntagvvfyav phrgadlgdf
      481 sasfkllllp svevqelgkd aphllevhek feklvkmfni pcisfgesak tilgfrmskv
      541 lvpvesadpg fgdffvvnts hleickphrw ssfsyqllld fldhafssis qdaywaahnp
      601 tlsrlrqlel yfetgvfqf
//
LOCUS       XP_077497973             619 aa            linear   INV 25-NOV-2025
DEFINITION  protein SERAC1-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077497973
VERSION     XP_077497973.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641847.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..619
                     /product="protein SERAC1-like isoform X4"
                     /calculated_mol_wt=69475
     Region          <383..473
                     /region_name="EstA"
                     /note="Triacylglycerol esterase/lipase EstA, alpha/beta
                     hydrolase fold [Lipid transport and metabolism]; COG1075"
                     /db_xref="CDD:440693"
     CDS             1..619
                     /gene="LOC144108642"
                     /coded_by="XM_077641847.1:129..1988"
                     /db_xref="GeneID:144108642"
ORIGIN      
        1 mshlygerps aalnespyic fsgksapate aspswslkhi frkearphwh llsearskvq
       61 rvrheavkal gsmtslsdse lceiaqacdc qtsvglarta gadlrcflsp ppipdyiakd
      121 slqdcmrmvl srlpetapes citfftknal qtklqyfdah hekedslrwr rpavgqaell
      181 clqallshta lpehrrllve klavlpllqr vlhehthdva lhslvaqmla nlaldpalhe
      241 pifragwvgi larwlrspev llsfpagral anmdtdalsp teywdgvyll hpqyrnreps
      301 vtadiifvhg llgsvfktwr qhdsqgtlnk leslleeptv savrssacki mvdhtsprar
      361 amhqkytkcw pkswlaedip ntrilaldyd tylsqwspnc plesdkrtlv fratdmlakl
      421 hragvggrpi iwithsmggl lvkqmlavas espqyeamvr ntagvvfyav phrgadlgdf
      481 sasfkllllp svevqelgkd aphllevhek feklvkmfni pcisfgesak tilgfrmskv
      541 lvpvesadpg fgdffvvnts hleickphrw ssfsyqllld fldhafssis qdaywaahnp
      601 tlsrlrqlel yfetgvfqf
//
LOCUS       XP_077497976             618 aa            linear   INV 25-NOV-2025
DEFINITION  protein SERAC1-like isoform X5 [Amblyomma americanum].
ACCESSION   XP_077497976
VERSION     XP_077497976.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641850.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..618
                     /product="protein SERAC1-like isoform X5"
                     /calculated_mol_wt=69419
     Region          <382..472
                     /region_name="EstA"
                     /note="Triacylglycerol esterase/lipase EstA, alpha/beta
                     hydrolase fold [Lipid transport and metabolism]; COG1075"
                     /db_xref="CDD:440693"
     CDS             1..618
                     /gene="LOC144108642"
                     /coded_by="XM_077641850.1:163..2019"
                     /db_xref="GeneID:144108642"
ORIGIN      
        1 mshlygerps aalnespyic fsesapatea spswslkhif rkearphwhl lsearskvqr
       61 vrheavkalg smtslsdsel ceiaqacdcq tsvglartag adlrcflspp pipdyiakds
      121 lqdcmrmvls rlpetapesc itfftknalq tklqyfdahh ekedslrwrr pavgqaellc
      181 lqallshtal pehrrllvek lavlpllqrv lhehthdval hslvaqmlan laldpalhep
      241 ifragwvgil arwlrspevl lsfpagrala nmdtdalspt eywdgvyllh pqyrnrepsv
      301 tadiifvhgl lgsvfktwrq hdsqgtlnkl eslleeptvs avrssackim vdhtsprara
      361 mhqkytkcwp kswlaedipn trilaldydt ylsqwspncp lesdkrtlvf ratdmlaklh
      421 ragvggrpii withsmggll vkqmlavase spqyeamvrn tagvvfyavp hrgadlgdfs
      481 asfkllllps vevqelgkda phllevhekf eklvkmfnip cisfgesakt ilgfrmskvl
      541 vpvesadpgf gdffvvntsh leickphrws sfsyqllldf ldhafssisq daywaahnpt
      601 lsrlrqlely fetgvfqf
//
LOCUS       XP_077497981             618 aa            linear   INV 25-NOV-2025
DEFINITION  protein SERAC1-like isoform X5 [Amblyomma americanum].
ACCESSION   XP_077497981
VERSION     XP_077497981.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641855.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..618
                     /product="protein SERAC1-like isoform X5"
                     /calculated_mol_wt=69419
     Region          <382..472
                     /region_name="EstA"
                     /note="Triacylglycerol esterase/lipase EstA, alpha/beta
                     hydrolase fold [Lipid transport and metabolism]; COG1075"
                     /db_xref="CDD:440693"
     CDS             1..618
                     /gene="LOC144108642"
                     /coded_by="XM_077641855.1:89..1945"
                     /db_xref="GeneID:144108642"
ORIGIN      
        1 mshlygerps aalnespyic fsesapatea spswslkhif rkearphwhl lsearskvqr
       61 vrheavkalg smtslsdsel ceiaqacdcq tsvglartag adlrcflspp pipdyiakds
      121 lqdcmrmvls rlpetapesc itfftknalq tklqyfdahh ekedslrwrr pavgqaellc
      181 lqallshtal pehrrllvek lavlpllqrv lhehthdval hslvaqmlan laldpalhep
      241 ifragwvgil arwlrspevl lsfpagrala nmdtdalspt eywdgvyllh pqyrnrepsv
      301 tadiifvhgl lgsvfktwrq hdsqgtlnkl eslleeptvs avrssackim vdhtsprara
      361 mhqkytkcwp kswlaedipn trilaldydt ylsqwspncp lesdkrtlvf ratdmlaklh
      421 ragvggrpii withsmggll vkqmlavase spqyeamvrn tagvvfyavp hrgadlgdfs
      481 asfkllllps vevqelgkda phllevhekf eklvkmfnip cisfgesakt ilgfrmskvl
      541 vpvesadpgf gdffvvntsh leickphrws sfsyqllldf ldhafssisq daywaahnpt
      601 lsrlrqlely fetgvfqf
//
LOCUS       XP_077497986             502 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108684 [Amblyomma americanum].
ACCESSION   XP_077497986
VERSION     XP_077497986.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641860.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..502
                     /product="uncharacterized protein LOC144108684"
                     /calculated_mol_wt=55735
     Region          <266..>362
                     /region_name="Mplasa_alph_rch"
                     /note="helix-rich Mycoplasma protein; TIGR04523"
                     /db_xref="CDD:275316"
     CDS             1..502
                     /gene="LOC144108684"
                     /coded_by="XM_077641860.1:7..1515"
                     /db_xref="GeneID:144108684"
ORIGIN      
        1 msvtnpfaff aqvflgllll peappsayqa svtilrttgg degipafrpa tfhqrdyris
       61 ygttrlstet tnastrtsgp ssikcgfags fgsgrggnak msvtnpfaff aqvflgllll
      121 peappsayqa svtilrttgg degipafrpa tfhqrdyris ygttrlstet tnastrtsgp
      181 ssiqcgfags fgsgrggnak msvtnpfaff aqvskrygks frsnnvclll lpcpqplcel
      241 llnvivslry qlllggdiee npgpidqtis eqlkiiaedi qeikkgkavt nqkliaiekk
      301 lekisgletq ltecarkvte lernlatmts kldelenrsr rsnliiygvk eqedettdtl
      361 hetvtktvfe dvlgitktdi erihrlgrps kdnetrprpi ilkfldyrdk lsilkqcskl
      421 kdsgfsiged fslavrnvrk klwdrtkehr drkekvslvy dkvkingqlf mwdsessdif
      481 pvkarnedqn ntgkritrsk rq
//
LOCUS       XP_077497992             265 aa            linear   INV 25-NOV-2025
DEFINITION  dihydropteridine reductase isoform X1 [Amblyomma americanum].
ACCESSION   XP_077497992
VERSION     XP_077497992.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641866.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..265
                     /product="dihydropteridine reductase isoform X1"
                     /calculated_mol_wt=28137
     Region          60..256
                     /region_name="DHPR_SDR_c_like"
                     /note="dihydropteridine reductase (DHPR), classical (c)
                     SDRs; cd05334"
                     /db_xref="CDD:187595"
     Site            order(88,118..121,124..126,128..129,140,144,147,164,
                     166..169,172,175..176,180,183..184,186..188,190..194)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187595"
     Site            order(174,178)
                     /site_type="active"
                     /db_xref="CDD:187595"
     CDS             1..265
                     /gene="Dhpr"
                     /coded_by="XM_077641866.1:16..813"
                     /db_xref="GeneID:144108694"
ORIGIN      
        1 mvrqpagcps vvnkskrpin aahvtncker wgrrnrlgcl gffrrpvsas rkptpgeytw
       61 vasvdhlpne eadanvvvqp cdawasqher vlegvdkvlq dgkidalicv aggwaggnaa
      121 svdyvkncds lwkssvwtsv iasslaskyl keggllaltg akaaleptpg migygmakaa
      181 vhhlvqslag aksglpqgat vvailpvtld tpmnrkfmpk adfsswtpls fvaelffkwt
      241 agqerpangs lvklvteggr tsvsp
//
LOCUS       XP_077497997             233 aa            linear   INV 25-NOV-2025
DEFINITION  dihydropteridine reductase isoform X2 [Amblyomma americanum].
ACCESSION   XP_077497997
VERSION     XP_077497997.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641871.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..233
                     /product="dihydropteridine reductase isoform X2"
                     /calculated_mol_wt=24411
     Region          2..224
                     /region_name="DHPR_SDR_c_like"
                     /note="dihydropteridine reductase (DHPR), classical (c)
                     SDRs; cd05334"
                     /db_xref="CDD:187595"
     Site            order(8..9,11..14,33,46,55,78..81,102,106,127..128,142,
                     146,174..177,179,182)
                     /site_type="other"
                     /note="NAD binding site [chemical binding]"
                     /db_xref="CDD:187595"
     Site            order(56,86..89,92..94,96..97,108,112,115,132,134..137,
                     140,143..144,148,151..152,154..156,158..162)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187595"
     Site            order(142,146)
                     /site_type="active"
                     /db_xref="CDD:187595"
     CDS             1..233
                     /gene="Dhpr"
                     /coded_by="XM_077641871.1:126..827"
                     /db_xref="GeneID:144108694"
ORIGIN      
        1 magrvvvygg kgalgsaivs ffrqkqwwva svdhlpneea danvvvqpcd awasqhervl
       61 egvdkvlqdg kidalicvag gwaggnaasv dyvkncdslw kssvwtsvia sslaskylke
      121 ggllaltgak aaleptpgmi gygmakaavh hlvqslagak sglpqgatvv ailpvtldtp
      181 mnrkfmpkad fsswtplsfv aelffkwtag qerpangslv klvteggrts vsp
//
LOCUS       XP_077498006             509 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108707 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498006
VERSION     XP_077498006.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641880.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..509
                     /product="uncharacterized protein LOC144108707 isoform X1"
                     /calculated_mol_wt=55539
     Region          <256..402
                     /region_name="LGT"
                     /note="Prolipoprotein diacylglyceryl transferase; cl00478"
                     /db_xref="CDD:469786"
     CDS             1..509
                     /gene="LOC144108707"
                     /coded_by="XM_077641880.1:166..1695"
                     /db_xref="GeneID:144108707"
ORIGIN      
        1 mfalvrfved dkdkryvisv adienfepkn eldfdntkty daywqdeded nsgiyvvqil
       61 klaatkeeme remrvkrvhv ppirmsevdn vtecgvpaaa rkkslkqqek akqqnqitsk
      121 kqqyedvlkr hvshslqkna aipghssigr kaskkrkpqi lddstesede svvssrelkn
      181 avkeakywkq ryrleqehta slkreldflk rkvelqlssf qqtldevakr qqdlcadalp
      241 vvapsilpng aveasrrdpt aargaateqa aaaekdvgga asvvptpagp seeapasscf
      301 tadaprsael easvlddaqd ftltsgglap mdvaesqetl agpsqqepmd vtesqetlag
      361 psqqepmdvt esqetlagps qqepmdvtes qetlagpsqq epmdvtesqe tlagpsqqep
      421 mdvtesqetl agpsqqapmd vtesqetlpg psqqasmdva tmldiqpgps qafqltselp
      481 tlptapvsvg tprirrpamv ngpvkcayf
//
LOCUS       XP_077498010             505 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108707 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077498010
VERSION     XP_077498010.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641884.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..505
                     /product="uncharacterized protein LOC144108707 isoform X2"
                     /calculated_mol_wt=55136
     Region          <252..398
                     /region_name="LGT"
                     /note="Prolipoprotein diacylglyceryl transferase; cl00478"
                     /db_xref="CDD:469786"
     CDS             1..505
                     /gene="LOC144108707"
                     /coded_by="XM_077641884.1:166..1683"
                     /db_xref="GeneID:144108707"
ORIGIN      
        1 mfalvrfved dkdkryvisv adienfepkn eldfdntkty daywqdeded nsgiyvvqil
       61 klaatkeeme remrvkrvhv ppirmsevdn vtecgvpaaa rkkslkqqek akqqnqitsk
      121 kqqyedvlkr hvshslqkna aipghssigr kaskkrkpqi lddstesede svvssrelkn
      181 avkeakywkq ryrleqehta slkreldflk rkvelqlssf qqtldevakr qqdalpvvap
      241 silpngavea srrdptaarg aateqaaaae kdvggaasvv ptpagpseea passcftada
      301 prsaeleasv lddaqdftlt sgglapmdva esqetlagps qqepmdvtes qetlagpsqq
      361 epmdvtesqe tlagpsqqep mdvtesqetl agpsqqepmd vtesqetlag psqqepmdvt
      421 esqetlagps qqapmdvtes qetlpgpsqq asmdvatmld iqpgpsqafq ltselptlpt
      481 apvsvgtpri rrpamvngpv kcayf
//
LOCUS       XP_077498018             503 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108707 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077498018
VERSION     XP_077498018.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641892.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..503
                     /product="uncharacterized protein LOC144108707 isoform X3"
                     /calculated_mol_wt=57936
     CDS             1..503
                     /gene="LOC144108707"
                     /coded_by="XM_077641892.1:166..1677"
                     /db_xref="GeneID:144108707"
ORIGIN      
        1 mfalvrfved dkdkryvisv adienfepkn eldfdntkty daywqdeded nsgiyvvqil
       61 klaatkeeme remrvkrvhv ppirmsevdn vtecgvpaaa rkkslkqqek akqqnqitsk
      121 kqqyedvlkr hvshslqkna aipghssigr kaskkrkpqi lddstesede svvssrelkn
      181 avkeakywkq ryrleqehta slkreldflk rkvelqlssf qqtldevakr qqdlcadalp
      241 vvapsilpng aveasrrdpt aargaateqa aaaekdvgga asvvptpagp seeapasscf
      301 tadaprsael easvlddaqd ftltsgglap mdvaesqetl agpsqqpgdw qahhnrnlwm
      361 slkarrlwqa hhnrnlwmsl karrlwqahh nrnlwmslka rrlwqahhnr nlwmslkarr
      421 lwqahhnrnl wmslkarrlw qahhnrhlwm slkarklyqa hhnrhlwmwp qcwtfsqghl
      481 kpfnlhqnfr lcqppqsllg rhe
//
LOCUS       XP_077498023             496 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108707 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077498023
VERSION     XP_077498023.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641897.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..496
                     /product="uncharacterized protein LOC144108707 isoform X4"
                     /calculated_mol_wt=54014
     Region          <243..389
                     /region_name="LGT"
                     /note="Prolipoprotein diacylglyceryl transferase; cl00478"
                     /db_xref="CDD:469786"
     CDS             1..496
                     /gene="LOC144108707"
                     /coded_by="XM_077641897.1:166..1656"
                     /db_xref="GeneID:144108707"
ORIGIN      
        1 mfalvrfved dkdkryvisv adienfepkn eldfdntkty daywqdeded nsgiyvvqil
       61 klaatkeeme remrvkrvhv ppirmsevdn vtecgvpaaa rkkslkqqek akqqnqitsk
      121 kqqyedvlkr hvshslqkna aipghssigr kaskkrkpqi lddstesede svvssrelkn
      181 avkeakywkq ryrleqehta slkreldflk rkvelqlssy lcadalpvva psilpngave
      241 asrrdptaar gaateqaaaa ekdvggaasv vptpagpsee apasscftad aprsaeleas
      301 vlddaqdftl tsgglapmdv aesqetlagp sqqepmdvte sqetlagpsq qepmdvtesq
      361 etlagpsqqe pmdvtesqet lagpsqqepm dvtesqetla gpsqqepmdv tesqetlagp
      421 sqqapmdvte sqetlpgpsq qasmdvatml diqpgpsqaf qltselptlp tapvsvgtpr
      481 irrpamvngp vkcayf
//
LOCUS       XP_077498026             492 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108707 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077498026
VERSION     XP_077498026.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641900.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..492
                     /product="uncharacterized protein LOC144108707 isoform X5"
                     /calculated_mol_wt=53612
     Region          <239..385
                     /region_name="LGT"
                     /note="Prolipoprotein diacylglyceryl transferase; cl00478"
                     /db_xref="CDD:469786"
     CDS             1..492
                     /gene="LOC144108707"
                     /coded_by="XM_077641900.1:165..1643"
                     /db_xref="GeneID:144108707"
ORIGIN      
        1 mfalvrfved dkdkryvisv adienfepkn eldfdntkty daywqdeded nsgiyvvqil
       61 klaatkeeme remrvkrvhv ppirmsevdn vtecgvpaaa rkkslkqqek akqqnqitsk
      121 kqqyedvlkr hvshslqkna aipghssigr kaskkrkpqi lddstesede svvssrelkn
      181 avkeakywkq ryrleqehta slkreldflk rkvelqlssy alpvvapsil pngaveasrr
      241 dptaargaat eqaaaaekdv ggaasvvptp agpseeapas scftadaprs aeleasvldd
      301 aqdftltsgg lapmdvaesq etlagpsqqe pmdvtesqet lagpsqqepm dvtesqetla
      361 gpsqqepmdv tesqetlagp sqqepmdvte sqetlagpsq qepmdvtesq etlagpsqqa
      421 pmdvtesqet lpgpsqqasm dvatmldiqp gpsqafqlts elptlptapv svgtprirrp
      481 amvngpvkca yf
//
LOCUS       XP_077498030             491 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108707 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077498030
VERSION     XP_077498030.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641904.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..491
                     /product="uncharacterized protein LOC144108707 isoform X6"
                     /calculated_mol_wt=53610
     Region          <256..402
                     /region_name="LGT"
                     /note="Prolipoprotein diacylglyceryl transferase; cl00478"
                     /db_xref="CDD:469786"
     CDS             1..491
                     /gene="LOC144108707"
                     /coded_by="XM_077641904.1:166..1641"
                     /db_xref="GeneID:144108707"
ORIGIN      
        1 mfalvrfved dkdkryvisv adienfepkn eldfdntkty daywqdeded nsgiyvvqil
       61 klaatkeeme remrvkrvhv ppirmsevdn vtecgvpaaa rkkslkqqek akqqnqitsk
      121 kqqyedvlkr hvshslqkna aipghssigr kaskkrkpqi lddstesede svvssrelkn
      181 avkeakywkq ryrleqehta slkreldflk rkvelqlssf qqtldevakr qqdlcadalp
      241 vvapsilpng aveasrrdpt aargaateqa aaaekdvgga asvvptpagp seeapasscf
      301 tadaprsael easvlddaqd ftltsgglap mdvaesqetl agpsqqepmd vtesqetlag
      361 psqqepmdvt esqetlagps qqepmdvtes qetlagpsqq epmdvtesqe tlagpsqqap
      421 mdvtesqetl pgpsqqasmd vatmldiqpg psqafqltse lptlptapvs vgtprirrpa
      481 mvngpvkcay f
//
LOCUS       XP_077498035             473 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108707 isoform X7 [Amblyomma
            americanum].
ACCESSION   XP_077498035
VERSION     XP_077498035.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641909.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..473
                     /product="uncharacterized protein LOC144108707 isoform X7"
                     /calculated_mol_wt=51681
     Region          <256..401
                     /region_name="LGT"
                     /note="Prolipoprotein diacylglyceryl transferase; cl00478"
                     /db_xref="CDD:469786"
     CDS             1..473
                     /gene="LOC144108707"
                     /coded_by="XM_077641909.1:166..1587"
                     /db_xref="GeneID:144108707"
ORIGIN      
        1 mfalvrfved dkdkryvisv adienfepkn eldfdntkty daywqdeded nsgiyvvqil
       61 klaatkeeme remrvkrvhv ppirmsevdn vtecgvpaaa rkkslkqqek akqqnqitsk
      121 kqqyedvlkr hvshslqkna aipghssigr kaskkrkpqi lddstesede svvssrelkn
      181 avkeakywkq ryrleqehta slkreldflk rkvelqlssf qqtldevakr qqdlcadalp
      241 vvapsilpng aveasrrdpt aargaateqa aaaekdvgga asvvptpagp seeapasscf
      301 tadaprsael easvlddaqd ftltsgglap mdvaesqetl agpsqqepmd vtesqetlag
      361 psqqepmdvt esqetlagps qqepmdvtes qetlagpsqq apmdvtesqe tlpgpsqqas
      421 mdvatmldiq pgpsqafqlt selptlptap vsvgtprirr pamvngpvkc ayf
//
LOCUS       XP_077498039             467 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108707 isoform X8 [Amblyomma
            americanum].
ACCESSION   XP_077498039
VERSION     XP_077498039.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641913.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..467
                     /product="uncharacterized protein LOC144108707 isoform X8"
                     /calculated_mol_wt=53336
     CDS             1..467
                     /gene="LOC144108707"
                     /coded_by="XM_077641913.1:167..1570"
                     /db_xref="GeneID:144108707"
ORIGIN      
        1 mfalvrfved dkdkryvisv adienfepkn eldfdntkty daywqdeded nsgiyvvqil
       61 klaatkeeme remrvkrvhv ppirmsevdn vtecgvpaaa rkkslkqqek akqqnqitsk
      121 kqqyedvlkr hvshslqkna aipghssigr kaskkrkpqi lddstesede svvssrelkn
      181 avkeakywkq ryrleqehta slkreldflk rkvelqlssf qqtldevakr qqdlcadalp
      241 vvapsilpng aveasrrdpt aargaateqa aaaekdvgga asvvptpagp seeapasscf
      301 tadaprsael easvlddaqd ftltsgglap mdvaesqetl agpsqqpgdw qahhnrnlwm
      361 slkarrlwqa hhnrnlwmsl karrlwqahh nrnlwmslka rrlwqahhnr hlwmslkark
      421 lyqahhnrhl wmwpqcwtfs qghlkpfnlh qnfrlcqppq sllgrhe
//
LOCUS       XP_077498047             465 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108707 isoform X9 [Amblyomma
            americanum].
ACCESSION   XP_077498047
VERSION     XP_077498047.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641921.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..465
                     /product="uncharacterized protein LOC144108707 isoform X9"
                     /calculated_mol_wt=50531
     Region          <212..358
                     /region_name="LGT"
                     /note="Prolipoprotein diacylglyceryl transferase; cl00478"
                     /db_xref="CDD:469786"
     CDS             1..465
                     /gene="LOC144108707"
                     /coded_by="XM_077641921.1:168..1565"
                     /db_xref="GeneID:144108707"
ORIGIN      
        1 mfalvrfved dkdkryvisv adienfepkn eldfdntkty daywqdeded nsgiyvvqil
       61 klaatkeeme remrvkrvhv ppirmsevdn vtecgvpaaa rkkslkqask krkpqildds
      121 tesedesvvs srelknavke akywkqryrl eqehtaslkr eldflkrkve lqlssfqqtl
      181 devakrqqdl cadalpvvap silpngavea srrdptaarg aateqaaaae kdvggaasvv
      241 ptpagpseea passcftada prsaeleasv lddaqdftlt sgglapmdva esqetlagps
      301 qqepmdvtes qetlagpsqq epmdvtesqe tlagpsqqep mdvtesqetl agpsqqepmd
      361 vtesqetlag psqqepmdvt esqetlagps qqapmdvtes qetlpgpsqq asmdvatmld
      421 iqpgpsqafq ltselptlpt apvsvgtpri rrpamvngpv kcayf
//
LOCUS       XP_077498055             455 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108707 isoform X10 [Amblyomma
            americanum].
ACCESSION   XP_077498055
VERSION     XP_077498055.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641929.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..455
                     /product="uncharacterized protein LOC144108707 isoform
                     X10"
                     /calculated_mol_wt=49752
     Region          <256..401
                     /region_name="LGT"
                     /note="Prolipoprotein diacylglyceryl transferase; cl00478"
                     /db_xref="CDD:469786"
     CDS             1..455
                     /gene="LOC144108707"
                     /coded_by="XM_077641929.1:165..1532"
                     /db_xref="GeneID:144108707"
ORIGIN      
        1 mfalvrfved dkdkryvisv adienfepkn eldfdntkty daywqdeded nsgiyvvqil
       61 klaatkeeme remrvkrvhv ppirmsevdn vtecgvpaaa rkkslkqqek akqqnqitsk
      121 kqqyedvlkr hvshslqkna aipghssigr kaskkrkpqi lddstesede svvssrelkn
      181 avkeakywkq ryrleqehta slkreldflk rkvelqlssf qqtldevakr qqdlcadalp
      241 vvapsilpng aveasrrdpt aargaateqa aaaekdvgga asvvptpagp seeapasscf
      301 tadaprsael easvlddaqd ftltsgglap mdvaesqetl agpsqqepmd vtesqetlag
      361 psqqepmdvt esqetlagps qqapmdvtes qetlpgpsqq asmdvatmld iqpgpsqafq
      421 ltselptlpt apvsvgtpri rrpamvngpv kcayf
//
LOCUS       XP_077498062             455 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108707 isoform X11 [Amblyomma
            americanum].
ACCESSION   XP_077498062
VERSION     XP_077498062.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641936.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..455
                     /product="uncharacterized protein LOC144108707 isoform
                     X11"
                     /calculated_mol_wt=49752
     Region          <256..401
                     /region_name="LGT"
                     /note="Prolipoprotein diacylglyceryl transferase; cl00478"
                     /db_xref="CDD:469786"
     CDS             1..455
                     /gene="LOC144108707"
                     /coded_by="XM_077641936.1:166..1533"
                     /db_xref="GeneID:144108707"
ORIGIN      
        1 mfalvrfved dkdkryvisv adienfepkn eldfdntkty daywqdeded nsgiyvvqil
       61 klaatkeeme remrvkrvhv ppirmsevdn vtecgvpaaa rkkslkqqek akqqnqitsk
      121 kqqyedvlkr hvshslqkna aipghssigr kaskkrkpqi lddstesede svvssrelkn
      181 avkeakywkq ryrleqehta slkreldflk rkvelqlssf qqtldevakr qqdlcadalp
      241 vvapsilpng aveasrrdpt aargaateqa aaaekdvgga asvvptpagp seeapasscf
      301 tadaprsael easvlddaqd ftltsgglap mdvaesqetl agpsqqepmd vtesqetlag
      361 psqqepmdvt esqetlagps qqapmdvtes qetlpgpsqq asmdvatmld iqpgpsqafq
      421 ltselptlpt apvsvgtpri rrpamvngpv kcayf
//
LOCUS       XP_077498071             455 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108707 isoform X12 [Amblyomma
            americanum].
ACCESSION   XP_077498071
VERSION     XP_077498071.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641945.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..455
                     /product="uncharacterized protein LOC144108707 isoform
                     X12"
                     /calculated_mol_wt=49752
     Region          <256..401
                     /region_name="LGT"
                     /note="Prolipoprotein diacylglyceryl transferase; cl00478"
                     /db_xref="CDD:469786"
     CDS             1..455
                     /gene="LOC144108707"
                     /coded_by="XM_077641945.1:166..1533"
                     /db_xref="GeneID:144108707"
ORIGIN      
        1 mfalvrfved dkdkryvisv adienfepkn eldfdntkty daywqdeded nsgiyvvqil
       61 klaatkeeme remrvkrvhv ppirmsevdn vtecgvpaaa rkkslkqqek akqqnqitsk
      121 kqqyedvlkr hvshslqkna aipghssigr kaskkrkpqi lddstesede svvssrelkn
      181 avkeakywkq ryrleqehta slkreldflk rkvelqlssf qqtldevakr qqdlcadalp
      241 vvapsilpng aveasrrdpt aargaateqa aaaekdvgga asvvptpagp seeapasscf
      301 tadaprsael easvlddaqd ftltsgglap mdvaesqetl agpsqqepmd vtesqetlag
      361 psqqepmdvt esqetlagps qqapmdvtes qetlpgpsqq asmdvatmld iqpgpsqafq
      421 ltselptlpt apvsvgtpri rrpamvngpv kcayf
//
LOCUS       XP_077498079             449 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108707 isoform X13 [Amblyomma
            americanum].
ACCESSION   XP_077498079
VERSION     XP_077498079.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641953.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..449
                     /product="uncharacterized protein LOC144108707 isoform
                     X13"
                     /calculated_mol_wt=51037
     CDS             1..449
                     /gene="LOC144108707"
                     /coded_by="XM_077641953.1:166..1515"
                     /db_xref="GeneID:144108707"
ORIGIN      
        1 mfalvrfved dkdkryvisv adienfepkn eldfdntkty daywqdeded nsgiyvvqil
       61 klaatkeeme remrvkrvhv ppirmsevdn vtecgvpaaa rkkslkqqek akqqnqitsk
      121 kqqyedvlkr hvshslqkna aipghssigr kaskkrkpqi lddstesede svvssrelkn
      181 avkeakywkq ryrleqehta slkreldflk rkvelqlssf qqtldevakr qqdlcadalp
      241 vvapsilpng aveasrrdpt aargaateqa aaaekdvgga asvvptpagp seeapasscf
      301 tadaprsael easvlddaqd ftltsgglap mdvaesqetl agpsqqpgdw qahhnrnlwm
      361 slkarrlwqa hhnrnlwmsl karrlwqahh nrhlwmslka rklyqahhnr hlwmwpqcwt
      421 fsqghlkpfn lhqnfrlcqp pqsllgrhe
//
LOCUS       XP_077498086             449 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108707 isoform X14 [Amblyomma
            americanum].
ACCESSION   XP_077498086
VERSION     XP_077498086.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641960.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..449
                     /product="uncharacterized protein LOC144108707 isoform
                     X14"
                     /calculated_mol_wt=51037
     CDS             1..449
                     /gene="LOC144108707"
                     /coded_by="XM_077641960.1:166..1515"
                     /db_xref="GeneID:144108707"
ORIGIN      
        1 mfalvrfved dkdkryvisv adienfepkn eldfdntkty daywqdeded nsgiyvvqil
       61 klaatkeeme remrvkrvhv ppirmsevdn vtecgvpaaa rkkslkqqek akqqnqitsk
      121 kqqyedvlkr hvshslqkna aipghssigr kaskkrkpqi lddstesede svvssrelkn
      181 avkeakywkq ryrleqehta slkreldflk rkvelqlssf qqtldevakr qqdlcadalp
      241 vvapsilpng aveasrrdpt aargaateqa aaaekdvgga asvvptpagp seeapasscf
      301 tadaprsael easvlddaqd ftltsgglap mdvaesqetl agpsqqpgdw qahhnrnlwm
      361 slkarrlwqa hhnrnlwmsl karrlwqahh nrhlwmslka rklyqahhnr hlwmwpqcwt
      421 fsqghlkpfn lhqnfrlcqp pqsllgrhe
//
LOCUS       XP_077498094             437 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108707 isoform X15 [Amblyomma
            americanum].
ACCESSION   XP_077498094
VERSION     XP_077498094.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641968.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..437
                     /product="uncharacterized protein LOC144108707 isoform
                     X15"
                     /calculated_mol_wt=47823
     CDS             1..437
                     /gene="LOC144108707"
                     /coded_by="XM_077641968.1:165..1478"
                     /db_xref="GeneID:144108707"
ORIGIN      
        1 mfalvrfved dkdkryvisv adienfepkn eldfdntkty daywqdeded nsgiyvvqil
       61 klaatkeeme remrvkrvhv ppirmsevdn vtecgvpaaa rkkslkqqek akqqnqitsk
      121 kqqyedvlkr hvshslqkna aipghssigr kaskkrkpqi lddstesede svvssrelkn
      181 avkeakywkq ryrleqehta slkreldflk rkvelqlssf qqtldevakr qqdlcadalp
      241 vvapsilpng aveasrrdpt aargaateqa aaaekdvgga asvvptpagp seeapasscf
      301 tadaprsael easvlddaqd ftltsgglap mdvaesqetl agpsqqepmd vtesqetlag
      361 psqqapmdvt esqetlpgps qqasmdvatm ldiqpgpsqa fqltselptl ptapvsvgtp
      421 rirrpamvng pvkcayf
//
LOCUS       XP_077498099             437 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108707 isoform X16 [Amblyomma
            americanum].
ACCESSION   XP_077498099
VERSION     XP_077498099.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641973.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..437
                     /product="uncharacterized protein LOC144108707 isoform
                     X16"
                     /calculated_mol_wt=47823
     CDS             1..437
                     /gene="LOC144108707"
                     /coded_by="XM_077641973.1:165..1478"
                     /db_xref="GeneID:144108707"
ORIGIN      
        1 mfalvrfved dkdkryvisv adienfepkn eldfdntkty daywqdeded nsgiyvvqil
       61 klaatkeeme remrvkrvhv ppirmsevdn vtecgvpaaa rkkslkqqek akqqnqitsk
      121 kqqyedvlkr hvshslqkna aipghssigr kaskkrkpqi lddstesede svvssrelkn
      181 avkeakywkq ryrleqehta slkreldflk rkvelqlssf qqtldevakr qqdlcadalp
      241 vvapsilpng aveasrrdpt aargaateqa aaaekdvgga asvvptpagp seeapasscf
      301 tadaprsael easvlddaqd ftltsgglap mdvaesqetl agpsqqepmd vtesqetlag
      361 psqqapmdvt esqetlpgps qqasmdvatm ldiqpgpsqa fqltselptl ptapvsvgtp
      421 rirrpamvng pvkcayf
//
LOCUS       XP_077498109             406 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108800 [Amblyomma americanum].
ACCESSION   XP_077498109
VERSION     XP_077498109.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077641983.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..406
                     /product="uncharacterized protein LOC144108800"
                     /calculated_mol_wt=45773
     CDS             1..406
                     /gene="LOC144108800"
                     /coded_by="XM_077641983.1:150..1370"
                     /db_xref="GeneID:144108800"
ORIGIN      
        1 maprkrrkvy ldpgssnvvp kstrwildnr qdrvprtstv rvlpavpsrs ppddmsednh
       61 rssddehwss pqsddfsdvc tedtgmsdds ftpptspfsv egdwceeehc ensdagprys
      121 deklsvpfid ggtitreday mmlldltlkf glswtvveei qklfnnller kafpesrylf
      181 kkfcgveltd imfhfyctdc ksllaetqgs leqrqqlqvt cgvchvsyeg rnlvrtgsff
      241 vslpiekqls silasktasn avtaslsres ssdqmrditd ghhyhsvrkk admtahdltl
      301 tvnsdgspvf ksskysiwpv qvilnelppl lrwsnvmvpl lwygkehpsm tlllqafvrq
      361 letmnvsgiv wtsadkqvcy klvlpsghkc grycqvlprk slpgsh
//
LOCUS       XP_077498131             744 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier family 15 member 2-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498131
VERSION     XP_077498131.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642005.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..744
                     /product="solute carrier family 15 member 2-like isoform
                     X1"
                     /calculated_mol_wt=81038
     Region          14..>376
                     /region_name="MFS_SLC15A1_2_like"
                     /note="Solute carrier family 15 members 1 and 2, and
                     similar Major Facilitator Superfamily transporters;
                     cd17347"
                     /db_xref="CDD:340905"
     CDS             1..744
                     /gene="LOC144108821"
                     /coded_by="XM_077642005.1:28..2262"
                     /db_xref="GeneID:144108821"
ORIGIN      
        1 meaeqepeat kpypkavffi ignefcerfs yygmrtiltl flinvlmfee htaksvyhgf
       61 vmacyfspvl gamiadsylg kfrtifyisi vyavgnitla vsavpalvth twipmiglai
      121 iafgtggikp cvsafggdqf lpgqerqleq ffslfylsin agsllstfit pilrvqrclg
      181 qmfcfplafg vpaalmvlal vlfvigkply rivppagnvv irvlqciwha lsrkvrskes
      241 rahwldhadd vfeksliadi kdvlhvlvly aplpvfwalf dqqgsqwtlq ankmdgeilg
      301 yqvlpdqmql vnpllilvlv pvfsyvvypl fgrcnllnrp lqkitvggla aaaafavagc
      361 ldlalessae apvpagfsrv afvntlpcpv dvsgnelrlr lesgevlsvp elpraalqas
      421 aaapcelsva rleatafsea qshsiffled kqmllaalva ntrgsnshna evrvaytvre
      481 pvsavvfada sgsktyaaap thigatssyt natgltpgry tarllgsddy evaaceaslk
      541 ngghytvtft qqadggplss kswrrggsvs sakkkcrnts asstrwaasr rllapwmavi
      601 fryprhnsml rtttiikgel qrardpaaeq pvhvlpgspv rahhsrrghv frdgarvllf
      661 agaelhevga pgrlaadrgr gqphrghrrr gevlprrvvr ivhvqrlddl ghggvwhhgl
      721 llqvhraghr aaayrretga glgg
//
LOCUS       XP_077498138             740 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier family 15 member 2-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077498138
VERSION     XP_077498138.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642012.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..740
                     /product="solute carrier family 15 member 2-like isoform
                     X2"
                     /calculated_mol_wt=80581
     Region          10..>372
                     /region_name="MFS_SLC15A1_2_like"
                     /note="Solute carrier family 15 members 1 and 2, and
                     similar Major Facilitator Superfamily transporters;
                     cd17347"
                     /db_xref="CDD:340905"
     CDS             1..740
                     /gene="LOC144108821"
                     /coded_by="XM_077642012.1:153..2375"
                     /db_xref="GeneID:144108821"
ORIGIN      
        1 mepeatkpyp kavffiigne fcerfsyygm rtiltlflin vlmfeehtak svyhgfvmac
       61 yfspvlgami adsylgkfrt ifyisivyav gnitlavsav palvthtwip miglaiiafg
      121 tggikpcvsa fggdqflpgq erqleqffsl fylsinagsl lstfitpilr vqrclgqmfc
      181 fplafgvpaa lmvlalvlfv igkplyrivp pagnvvirvl qciwhalsrk vrskesrahw
      241 ldhaddvfek sliadikdvl hvlvlyaplp vfwalfdqqg sqwtlqankm dgeilgyqvl
      301 pdqmqlvnpl lilvlvpvfs yvvyplfgrc nllnrplqki tvgglaaaaa favagcldla
      361 lessaeapvp agfsrvafvn tlpcpvdvsg nelrlrlesg evlsvpelpr aalqasaaap
      421 celsvarlea tafseaqshs iffledkqml laalvantrg snshnaevrv aytvrepvsa
      481 vvfadasgsk tyaaapthig atssytnatg ltpgrytarl lgsddyevaa ceaslknggh
      541 ytvtftqqad ggplsskswr rggsvssakk kcrntsasst rwaasrrlla pwmavifryp
      601 rhnsmlrttt iikgelqrar dpaaeqpvhv lpgspvrahh srrghvfrdg arvllfagae
      661 lhevgapgrl aadrgrgqph rghrrrgevl prrvvrivhv qrlddlghgg vwhhglllqv
      721 hraghraaay rretgaglgg
//
LOCUS       XP_077498147             688 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier family 15 member 1-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077498147
VERSION     XP_077498147.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642021.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..688
                     /product="solute carrier family 15 member 1-like isoform
                     X3"
                     /calculated_mol_wt=74596
     Region          14..667
                     /region_name="MFS_SLC15A1_2_like"
                     /note="Solute carrier family 15 members 1 and 2, and
                     similar Major Facilitator Superfamily transporters;
                     cd17347"
                     /db_xref="CDD:340905"
     Site            order(27..28,31..32,35,68,125..126,128..130,133,156,
                     159..160,163,273,276..277,280..282,285,316,320,586..587,
                     591,595,611,614..615,618..619,622)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340905"
     CDS             1..688
                     /gene="LOC144108821"
                     /coded_by="XM_077642021.1:19..2085"
                     /db_xref="GeneID:144108821"
ORIGIN      
        1 meaeqepeat kpypkavffi ignefcerfs yygmrtiltl flinvlmfee htaksvyhgf
       61 vmacyfspvl gamiadsylg kfrtifyisi vyavgnitla vsavpalvth twipmiglai
      121 iafgtggikp cvsafggdqf lpgqerqleq ffslfylsin agsllstfit pilrvqrclg
      181 qmfcfplafg vpaalmvlal vlfvigkply rivppagnvv irvlqciwha lsrkvrskes
      241 rahwldhadd vfeksliadi kdvlhvlvly aplpvfwalf dqqgsqwtlq ankmdgeilg
      301 yqvlpdqmql vnpllilvlv pvfsyvvypl fgrcnllnrp lqkitvggla aaaafavagc
      361 ldlalessae apvpagfsrv afvntlpcpv dvsgnelrlr lesgevlsvp elpraalqas
      421 aaapcelsva rleatafsea qshsiffled kqmllaalva ntrgsnshna evrvaytvre
      481 pvsavvfada sgsktyaaap thigatssyt natgltpgry tarllgsddy evaaceaslk
      541 ngghytvtft qqadggpvnc svhetlrpns lsmfyqvpqy vlitagevmf svtglefsys
      601 qapssmksvl qaawlltvav gnlivvivae arffhvasse sfmysglmtl dmvvfgimac
      661 fykyiepgta pppivekpal asegdtkm
//
LOCUS       XP_077498155             684 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier family 15 member 1-like isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077498155
VERSION     XP_077498155.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642029.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..684
                     /product="solute carrier family 15 member 1-like isoform
                     X4"
                     /calculated_mol_wt=74139
     Region          10..663
                     /region_name="MFS_SLC15A1_2_like"
                     /note="Solute carrier family 15 members 1 and 2, and
                     similar Major Facilitator Superfamily transporters;
                     cd17347"
                     /db_xref="CDD:340905"
     Site            order(23..24,27..28,31,64,121..122,124..126,129,152,
                     155..156,159,269,272..273,276..278,281,312,316,582..583,
                     587,591,607,610..611,614..615,618)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340905"
     CDS             1..684
                     /gene="LOC144108821"
                     /coded_by="XM_077642029.1:153..2207"
                     /db_xref="GeneID:144108821"
ORIGIN      
        1 mepeatkpyp kavffiigne fcerfsyygm rtiltlflin vlmfeehtak svyhgfvmac
       61 yfspvlgami adsylgkfrt ifyisivyav gnitlavsav palvthtwip miglaiiafg
      121 tggikpcvsa fggdqflpgq erqleqffsl fylsinagsl lstfitpilr vqrclgqmfc
      181 fplafgvpaa lmvlalvlfv igkplyrivp pagnvvirvl qciwhalsrk vrskesrahw
      241 ldhaddvfek sliadikdvl hvlvlyaplp vfwalfdqqg sqwtlqankm dgeilgyqvl
      301 pdqmqlvnpl lilvlvpvfs yvvyplfgrc nllnrplqki tvgglaaaaa favagcldla
      361 lessaeapvp agfsrvafvn tlpcpvdvsg nelrlrlesg evlsvpelpr aalqasaaap
      421 celsvarlea tafseaqshs iffledkqml laalvantrg snshnaevrv aytvrepvsa
      481 vvfadasgsk tyaaapthig atssytnatg ltpgrytarl lgsddyevaa ceaslknggh
      541 ytvtftqqad ggpvncsvhe tlrpnslsmf yqvpqyvlit agevmfsvtg lefsysqaps
      601 smksvlqaaw lltvavgnli vvivaearff hvassesfmy sglmtldmvv fgimacfyky
      661 iepgtapppi vekpalaseg dtkm
//
LOCUS       XP_077498163             563 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108821 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077498163
VERSION     XP_077498163.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642037.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..563
                     /product="uncharacterized protein LOC144108821 isoform X5"
                     /calculated_mol_wt=60914
     Region          <3..>213
                     /region_name="2A1704"
                     /note="Peptide:H+ symporter (also transports b-lactam
                     antibiotics, the antitumor agent, bestatin, and various
                     protease inhibitors); TIGR00926"
                     /db_xref="CDD:273343"
     CDS             1..563
                     /gene="LOC144108821"
                     /coded_by="XM_077642037.1:147..1838"
                     /db_xref="GeneID:144108821"
ORIGIN      
        1 mfcfplafgv paalmvlalv lfvigkplyr ivppagnvvi rvlqciwhal srkvrskesr
       61 ahwldhaddv feksliadik dvlhvlvlya plpvfwalfd qqgsqwtlqa nkmdgeilgy
      121 qvlpdqmqlv npllilvlvp vfsyvvyplf grcnllnrpl qkitvgglaa aaafavagcl
      181 dlalessaea pvpagfsrva fvntlpcpvd vsgnelrlrl esgevlsvpe lpraalqasa
      241 aapcelsvar leatafseaq shsiffledk qmllaalvan trgsnshnae vrvaytvrep
      301 vsavvfadas gsktyaaapt higatssytn atgltpgryt arllgsddye vaaceaslkn
      361 gghytvtftq qadggplssk swrrggsvss akkkcrntsa sstrwaasrr llapwmavif
      421 ryprhnsmlr tttiikgelq rardpaaeqp vhvlpgspvr ahhsrrghvf rdgarvllfa
      481 gaelhevgap grlaadrgrg qphrghrrrg evlprrvvri vhvqrlddlg hggvwhhgll
      541 lqvhraghra aayrretgag lgg
//
LOCUS       XP_077498192             423 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108846 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498192
VERSION     XP_077498192.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642066.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..423
                     /product="uncharacterized protein LOC144108846 isoform X1"
                     /calculated_mol_wt=46033
     Region          160..205
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     Region          358..397
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     CDS             1..423
                     /gene="LOC144108846"
                     /coded_by="XM_077642066.1:56..1327"
                     /db_xref="GeneID:144108846"
ORIGIN      
        1 maecslarca qerrafrkef qrwsrdmlfv iglervaeem mgskrwnrmt qlenaleeql
       61 qlvdwvpdqv cyfcdaqpan eppgppsdys sfksfqmdld eqstgsdqas gspgspaave
      121 eqpldlslhg rpaqepptqq lgrpavrsgg egvggkrsyt eaelqaalrd iqsgklgtrr
      181 aaviygiprs tlrnkvykla lerrnsassa aapasdslrq llraapakpl ehspdlrplf
      241 aqflssiqql alqpasgaee pkapttlpvv sdlirmlaqe rldaaalrrp aadvilkvps
      301 yrpaaqragp aaasssssss ssssssssea aagsagggta apeekrtrpk rgryrnydrd
      361 hliqavravq rgemsvhrag tyfgvphstl eykvkerhll rpkkrprqpd gllealikst
      421 ldk
//
LOCUS       XP_077498199             371 aa            linear   INV 25-NOV-2025
DEFINITION  mblk-1-related factor 1-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077498199
VERSION     XP_077498199.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642073.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..371
                     /product="mblk-1-related factor 1-like isoform X2"
                     /calculated_mol_wt=39479
     Region          108..153
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     Region          306..345
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     CDS             1..371
                     /gene="LOC144108846"
                     /coded_by="XM_077642073.1:120..1235"
                     /db_xref="GeneID:144108846"
ORIGIN      
        1 mepgyafchr fgargrrndg lqavepddtt rkrlggaaaa grlgagpsvl llrratgers
       61 pgspaaveeq pldlslhgrp aqepptqqlg rpavrsggeg vggkrsytea elqaalrdiq
      121 sgklgtrraa viygiprstl rnkvyklale rrnsassaaa pasdslrqll raapakpleh
      181 spdlrplfaq flssiqqlal qpasgaeepk apttlpvvsd lirmlaqerl daaalrrpaa
      241 dvilkvpsyr paaqragpaa asssssssss sssssseaaa gsagggtaap eekrtrpkrg
      301 ryrnydrdhl iqavravqrg emsvhragty fgvphstley kvkerhllrp kkrprqpdgl
      361 lealikstld k
//
LOCUS       XP_077498207             194 aa            linear   INV 25-NOV-2025
DEFINITION  soma ferritin-like [Amblyomma americanum].
ACCESSION   XP_077498207
VERSION     XP_077498207.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642081.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..194
                     /product="soma ferritin-like"
                     /calculated_mol_wt=22115
     Region          31..189
                     /region_name="Euk_Ferritin"
                     /note="eukaryotic ferritins; cd01056"
                     /db_xref="CDD:153114"
     Site            order(45,52,79..80,83,125,160)
                     /site_type="other"
                     /note="ferroxidase diiron center [ion binding]"
                     /db_xref="CDD:153114"
     Site            order(75,78..79,82)
                     /site_type="active"
                     /note="ferrihydrite nucleation center [active]"
                     /db_xref="CDD:153114"
     Site            order(136,150,153)
                     /site_type="active"
                     /note="iron ion channel [active]"
                     /db_xref="CDD:153114"
     CDS             1..194
                     /gene="LOC144108856"
                     /coded_by="XM_077642081.1:113..697"
                     /db_xref="GeneID:144108856"
ORIGIN      
        1 mlrllvvasa laaagsnnld eqasqnkyfl hdrcrlglqe qinlelhasl vytqmaayla
       61 nnnvaragfa hffrhqsdee rehahklidy vnlrggtvst vnlqmpttat wmsvldvlqr
      121 alalehdvtn rlyelhrlad dhcrdpqmad fleqeflaeq mrsidqlqrl itqlqnmdtg
      181 lgeflldreq lrsp
//
LOCUS       XP_077498220             904 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidic Acid Phospholipase A1 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498220
VERSION     XP_077498220.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642094.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..904
                     /product="phosphatidic Acid Phospholipase A1 isoform X1"
                     /calculated_mol_wt=100411
     Region          <28..>171
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          174..243
                     /region_name="WWE"
                     /note="WWE domain; pfam02825"
                     /db_xref="CDD:460715"
     Region          527..582
                     /region_name="SAM_superfamily"
                     /note="SAM (Sterile alpha motif); cl15755"
                     /db_xref="CDD:472832"
     Region          630..836
                     /region_name="DDHD"
                     /note="DDHD domain; pfam02862"
                     /db_xref="CDD:460725"
     CDS             1..904
                     /gene="PAPLA1"
                     /coded_by="XM_077642094.1:144..2858"
                     /db_xref="GeneID:144108863"
ORIGIN      
        1 maglgrndpk kltslllqpp nssfswsdvp sgnilmpvsq iepvteggad aflgqepgqf
       61 pqapeqvgne pvptgpfsql qqplrppltq atldgpstap apqqvlpppp mgppinfhrq
      121 splthasllh qaspmpgpap mdqlpyppls feqpdhaqlp vsppgaaytp vkphwfysre
      181 iehklvwmpf slldstrlee asarlafgsd lmvttdggry dvdvgarlrr pvyweempsq
      241 vrrctwfykl egqnrfvpye eqvahvledh yrrsmatgnw hqrlelpgnq tvvmhnssvm
      301 vhflpsshpd ewgnvsegqs rprvvkrgvt evdtidegep sqidhlvfvv hgvgsvcdfr
      361 frsveecldd irhialglvr shfggplqeg rigrveflpv swhstlhsda tgidralrhi
      421 tlrsipklrh ftndtlldil fytspvycqt ivdtvgsein rlhskfmern pgfsgtiala
      481 ghslgslily dilshqcdtg dvfvaeqspv paaaepeels lqppqdpsve svfrelmlsd
      541 yisrikeeqm dmetlkmcse gdlremglpm gprkkllsym kdlsekqqpa rvaapqqqqv
      601 grkresvasv nyamgeagtg qlsvsypkin fhpscffalg spiamflvvr gnealgedfk
      661 lptcpaffni fhpfdpvayr menlidpnft acpvliphhk grkrmhlelk eslsrmgsdl
      721 kqrfmdslry twnslnefar ahrsspvqve aevdrvlqee isrqadaael estvtasdlq
      781 gvddlgvgql nggrridyvl qekpiesfne ylfalashac ywesedtvll ilkelyalqg
      841 ltpqlpssrs asqprvqlyn tgstqllhen caaaasglcp psanaatppp plqgfvrvsp
      901 ltrk
//
LOCUS       XP_077498226             903 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidic Acid Phospholipase A1 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077498226
VERSION     XP_077498226.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642100.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..903
                     /product="phosphatidic Acid Phospholipase A1 isoform X2"
                     /calculated_mol_wt=100287
     Region          <28..>171
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          174..243
                     /region_name="WWE"
                     /note="WWE domain; pfam02825"
                     /db_xref="CDD:460715"
     Region          527..582
                     /region_name="SAM_superfamily"
                     /note="SAM (Sterile alpha motif); cl15755"
                     /db_xref="CDD:472832"
     Region          629..835
                     /region_name="DDHD"
                     /note="DDHD domain; pfam02862"
                     /db_xref="CDD:460725"
     CDS             1..903
                     /gene="PAPLA1"
                     /coded_by="XM_077642100.1:144..2855"
                     /db_xref="GeneID:144108863"
ORIGIN      
        1 maglgrndpk kltslllqpp nssfswsdvp sgnilmpvsq iepvteggad aflgqepgqf
       61 pqapeqvgne pvptgpfsql qqplrppltq atldgpstap apqqvlpppp mgppinfhrq
      121 splthasllh qaspmpgpap mdqlpyppls feqpdhaqlp vsppgaaytp vkphwfysre
      181 iehklvwmpf slldstrlee asarlafgsd lmvttdggry dvdvgarlrr pvyweempsq
      241 vrrctwfykl egqnrfvpye eqvahvledh yrrsmatgnw hqrlelpgnq tvvmhnssvm
      301 vhflpsshpd ewgnvsegqs rprvvkrgvt evdtidegep sqidhlvfvv hgvgsvcdfr
      361 frsveecldd irhialglvr shfggplqeg rigrveflpv swhstlhsda tgidralrhi
      421 tlrsipklrh ftndtlldil fytspvycqt ivdtvgsein rlhskfmern pgfsgtiala
      481 ghslgslily dilshqcdtg dvfvaeqspv paaaepeels lqppqdpsve svfrelmlsd
      541 yisrikeeqm dmetlkmcse gdlremglpm gprkkllsym kdlsekqatr vaapqqqqvg
      601 rkresvasvn yamgeagtgq lsvsypkinf hpscffalgs piamflvvrg nealgedfkl
      661 ptcpaffnif hpfdpvayrm enlidpnfta cpvliphhkg rkrmhlelke slsrmgsdlk
      721 qrfmdslryt wnslnefara hrsspvqvea evdrvlqeei srqadaaele stvtasdlqg
      781 vddlgvgqln ggrridyvlq ekpiesfney lfalashacy wesedtvlli lkelyalqgl
      841 tpqlpssrsa sqprvqlynt gstqllhenc aaaasglcpp sanaatpppp lqgfvrvspl
      901 trk
//
LOCUS       XP_077498233             900 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidic Acid Phospholipase A1 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077498233
VERSION     XP_077498233.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642107.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..900
                     /product="phosphatidic Acid Phospholipase A1 isoform X3"
                     /calculated_mol_wt=99953
     Region          <28..>171
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          174..243
                     /region_name="WWE"
                     /note="WWE domain; pfam02825"
                     /db_xref="CDD:460715"
     Region          527..582
                     /region_name="SAM_superfamily"
                     /note="SAM (Sterile alpha motif); cl15755"
                     /db_xref="CDD:472832"
     Region          630..832
                     /region_name="DDHD"
                     /note="DDHD domain; pfam02862"
                     /db_xref="CDD:460725"
     CDS             1..900
                     /gene="PAPLA1"
                     /coded_by="XM_077642107.1:144..2846"
                     /db_xref="GeneID:144108863"
ORIGIN      
        1 maglgrndpk kltslllqpp nssfswsdvp sgnilmpvsq iepvteggad aflgqepgqf
       61 pqapeqvgne pvptgpfsql qqplrppltq atldgpstap apqqvlpppp mgppinfhrq
      121 splthasllh qaspmpgpap mdqlpyppls feqpdhaqlp vsppgaaytp vkphwfysre
      181 iehklvwmpf slldstrlee asarlafgsd lmvttdggry dvdvgarlrr pvyweempsq
      241 vrrctwfykl egqnrfvpye eqvahvledh yrrsmatgnw hqrlelpgnq tvvmhnssvm
      301 vhflpsshpd ewgnvsegqs rprvvkrgvt evdtidegep sqidhlvfvv hgvgsvcdfr
      361 frsveecldd irhialglvr shfggplqeg rigrveflpv swhstlhsda tgidralrhi
      421 tlrsipklrh ftndtlldil fytspvycqt ivdtvgsein rlhskfmern pgfsgtiala
      481 ghslgslily dilshqcdtg dvfvaeqspv paaaepeels lqppqdpsve svfrelmlsd
      541 yisrikeeqm dmetlkmcse gdlremglpm gprkkllsym kdlsekqqpa rvaapqqqqv
      601 grkresvasv nyamgeagtg qlsvsypkin fhpscffalg spiamflvvr gnealgedfk
      661 lptcpaffni fhpayrmenl idpnftacpv liphhkgrkr mhlelkesls rmgsdlkqrf
      721 mdslrytwns lnefarahrs spvqveaevd rvlqeeisrq adaaelestv tasdlqgvdd
      781 lgvgqlnggr ridyvlqekp iesfneylfa lashacywes edtvllilke lyalqgltpq
      841 lpssrsasqp rvqlyntgst qllhencaaa asglcppsan aatpppplqg fvrvspltrk
//
LOCUS       XP_077498244              85 aa            linear   INV 25-NOV-2025
DEFINITION  V-type proton ATPase subunit e 1-like [Amblyomma americanum].
ACCESSION   XP_077498244
VERSION     XP_077498244.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642118.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..85
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..85
                     /product="V-type proton ATPase subunit e 1-like"
                     /calculated_mol_wt=9265
     Region          <23..65
                     /region_name="ATP_synt_H"
                     /note="ATP synthase subunit H; pfam05493"
                     /db_xref="CDD:461663"
     CDS             1..85
                     /gene="LOC144108883"
                     /coded_by="XM_077642118.1:311..568"
                     /db_xref="GeneID:144108883"
ORIGIN      
        1 mgadvaavsi vsvfwvvvga vvpwfvrkgs hrsliqamiv ttsiccylfw lctymsqmyp
       61 mvgptlsnst vvlmdaywsh nasnv
//
LOCUS       XP_077498260             176 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108899 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498260
VERSION     XP_077498260.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642134.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..176
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..176
                     /product="uncharacterized protein LOC144108899 isoform X1"
                     /calculated_mol_wt=18913
     CDS             1..176
                     /gene="LOC144108899"
                     /coded_by="XM_077642134.1:600..1130"
                     /db_xref="GeneID:144108899"
ORIGIN      
        1 mkpyhldsrn tsmdvpvmtd mcqggsshvs tpcmevgvte slgalqtvfd giyedcdegh
       61 glvpkavifc pqtrqeglsg adskmevalw alqkrccmvr dersnrtegc sgnmvrldgk
      121 mslqfdgavt ptvlasaiaf aglplvvflg gmmrhrqstl plftdlkvpa livies
//
LOCUS       XP_077498265             155 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108899 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077498265
VERSION     XP_077498265.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642139.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..155
                     /product="uncharacterized protein LOC144108899 isoform X2"
                     /calculated_mol_wt=17796
     CDS             1..155
                     /gene="LOC144108899"
                     /coded_by="XM_077642139.1:950..1417"
                     /db_xref="GeneID:144108899"
ORIGIN      
        1 mmrdrfamaa flysydgysk ylvclprtls ylygsetctl ptwcrkpsgr skedifeacp
       61 ylydmlamse haeviqmhhe kyephmrnfv rdntrglrig lrselgsard lahvkddlgr
      121 hrrcglslqa lhgraaqrsh serrlsrsga siacy
//
LOCUS       XP_077498266             155 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108899 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077498266
VERSION     XP_077498266.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642140.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..155
                     /product="uncharacterized protein LOC144108899 isoform X2"
                     /calculated_mol_wt=17796
     CDS             1..155
                     /gene="LOC144108899"
                     /coded_by="XM_077642140.1:827..1294"
                     /db_xref="GeneID:144108899"
ORIGIN      
        1 mmrdrfamaa flysydgysk ylvclprtls ylygsetctl ptwcrkpsgr skedifeacp
       61 ylydmlamse haeviqmhhe kyephmrnfv rdntrglrig lrselgsard lahvkddlgr
      121 hrrcglslqa lhgraaqrsh serrlsrsga siacy
//
LOCUS       XP_077498267             176 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108928 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498267
VERSION     XP_077498267.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642141.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..176
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..176
                     /product="uncharacterized protein LOC144108928 isoform X1"
                     /calculated_mol_wt=18842
     CDS             1..176
                     /gene="LOC144108928"
                     /coded_by="XM_077642141.1:2609..3139"
                     /db_xref="GeneID:144108928"
ORIGIN      
        1 mkpyhldsrn tsmdvpvmtd mcqggsshvs tpcmevgvte slgalqtvfd gnyedcdegr
       61 glvpkavifc pqtrqeglsg adskkevalw alqkrccmvr dersnrtegc sgnmvrvggk
      121 mslkfdgavt ptvlasaiaf vglplvvflg gmmrhrqsal plftdlkvpa livves
//
LOCUS       XP_077498268             176 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108928 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498268
VERSION     XP_077498268.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642142.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..176
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..176
                     /product="uncharacterized protein LOC144108928 isoform X1"
                     /calculated_mol_wt=18842
     CDS             1..176
                     /gene="LOC144108928"
                     /coded_by="XM_077642142.1:431..961"
                     /db_xref="GeneID:144108928"
ORIGIN      
        1 mkpyhldsrn tsmdvpvmtd mcqggsshvs tpcmevgvte slgalqtvfd gnyedcdegr
       61 glvpkavifc pqtrqeglsg adskkevalw alqkrccmvr dersnrtegc sgnmvrvggk
      121 mslkfdgavt ptvlasaiaf vglplvvflg gmmrhrqsal plftdlkvpa livves
//
LOCUS       XP_077498269             149 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108928 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077498269
VERSION     XP_077498269.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642143.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..149
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..149
                     /product="uncharacterized protein LOC144108928 isoform X2"
                     /calculated_mol_wt=15970
     CDS             1..149
                     /gene="LOC144108928"
                     /coded_by="XM_077642143.1:158..607"
                     /db_xref="GeneID:144108928"
ORIGIN      
        1 mlpqvfvsek nlfgdplars glhpcihvll sfggnvlqes rsetstrqeg lsgadskkev
       61 alwalqkrcc mvrdersnrt egcsgnmvrv ggkmslkfdg avtptvlasa iafvglplvv
      121 flggmmrhrq salplftdlk vpalivves
//
LOCUS       XP_077498270             318 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144108974 [Amblyomma americanum].
ACCESSION   XP_077498270
VERSION     XP_077498270.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642144.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..318
                     /product="uncharacterized protein LOC144108974"
                     /calculated_mol_wt=35405
     CDS             1..318
                     /gene="LOC144108974"
                     /coded_by="XM_077642144.1:1846..2802"
                     /db_xref="GeneID:144108974"
ORIGIN      
        1 mmrdrfamaa flyaydgysk ylvclprtls ylygsetctl ptwcrkpscr skedifeaca
       61 ylydmlamsq haeviqmhhe kyepdmrnfv rdntlgacvw vfalslvvll ilrmlkmiwg
      121 diaavvhlyk rytaerrkgv trngafhava lallatdtsp meallagkeq rckvisilgp
      181 itasravsah nvghnqkcpd vnpavapevv dlhgapessd alhpsldnas qshvlvevrq
      241 tlkpqgapqh crrftgfdan iflndaedpf msrhqigrac vvrivspvtk raalatrlal
      301 rlvmfritts aasrplql
//
LOCUS       XP_077498271             205 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109016 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498271
VERSION     XP_077498271.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642145.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..205
                     /product="uncharacterized protein LOC144109016 isoform X1"
                     /calculated_mol_wt=22037
     CDS             1..205
                     /gene="LOC144109016"
                     /coded_by="XM_077642145.1:43..660"
                     /db_xref="GeneID:144109016"
ORIGIN      
        1 mlkmiwgdia avvhlykryt aerrkgvtrn gafhavalal latdtspiea llagkeqrck
       61 visilgpita spavsahnvg hnqkcpvvnp avapevvdlh gapessdalh psldnasqsh
      121 vldevrqtlk pqgapqhccr ftgfdanifl ndaedpfmsr hqigracvvr ivspvtkraa
      181 latrlalrlv mfritsssas rplql
//
LOCUS       XP_077498272             147 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109016 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077498272
VERSION     XP_077498272.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642146.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..147
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..147
                     /product="uncharacterized protein LOC144109016 isoform X2"
                     /calculated_mol_wt=15827
     CDS             1..147
                     /gene="LOC144109016"
                     /coded_by="XM_077642146.1:134..577"
                     /db_xref="GeneID:144109016"
ORIGIN      
        1 mlpqvfvsek nlfgdplars glhpcihvlf sfggnvlqes rsetstrqeg lsgadskkev
       61 alwalqkrcc mvrdersnrt egcsgnmvrl dgnvslqfdg avtptvlasa iafvglplvv
      121 flggmmrhrq salplftdlk vpalivi
//
LOCUS       XP_077498273             144 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109016 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077498273
VERSION     XP_077498273.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642147.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..144
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..144
                     /product="uncharacterized protein LOC144109016 isoform X3"
                     /calculated_mol_wt=16013
     CDS             1..144
                     /gene="LOC144109016"
                     /coded_by="XM_077642147.1:1389..1823"
                     /db_xref="GeneID:144109016"
ORIGIN      
        1 mpevtpltvs slhlnnseil rrtfeilvss nhvtslrvcl qkcfldkiqa amaayimear
       61 tlkdlevhay vdepeledqq hdpdagkhlv nalasnlslt ritvrelpls ednceflans
      121 fansqnlsel siavlsrdsy kafl
//
LOCUS       XP_077498274             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109016 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077498274
VERSION     XP_077498274.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642148.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144109016 isoform X4"
                     /calculated_mol_wt=15056
     CDS             1..141
                     /gene="LOC144109016"
                     /coded_by="XM_077642148.1:39..464"
                     /db_xref="GeneID:144109016"
ORIGIN      
        1 mevgvtesvg alqtvfdgny edcdegrglv pkavifcpqt rqeglsgads kkevalwalq
       61 krccmvrder snrtegcsgn mvrldgnvsl qfdgavtptv lasaiafvgl plvvflggmm
      121 rhrqsalplf tdlkvpaliv i
//
LOCUS       XP_077498275              99 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109016 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077498275
VERSION     XP_077498275.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642149.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..99
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..99
                     /product="uncharacterized protein LOC144109016 isoform X5"
                     /calculated_mol_wt=11492
     CDS             1..99
                     /gene="LOC144109016"
                     /coded_by="XM_077642149.1:2767..3066"
                     /db_xref="GeneID:144109016"
ORIGIN      
        1 mmrdrfamaa flyaydgyik ylvclprtls ylygsetcal ptwcrkpsgr skedifeaca
       61 ylydmlamse haeviqmhhe kyephmrnyv rdntlgacv
//
LOCUS       XP_077498276             257 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109072 [Amblyomma americanum].
ACCESSION   XP_077498276
VERSION     XP_077498276.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642150.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..257
                     /product="uncharacterized protein LOC144109072"
                     /calculated_mol_wt=28631
     Region          21..109
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..257
                     /gene="LOC144109072"
                     /coded_by="XM_077642150.1:139..912"
                     /db_xref="GeneID:144109072"
ORIGIN      
        1 maatmsveps amaseassqk rvnwptvtte aliriwednl sslrsntrna riynamaell
       61 naglpsgegp ytakqvrqkl enlnkqyrkl rrsgsstgsk gvqwpfywql hsflgtlpvn
      121 dsdlmqesve mpedpahtgq eivfeyednl ipvpatipct leegndstag sepagreqgt
      181 sgercgksnl rrkrpsssll relmamhqqa eeraakaake sselrkqlie lqkesnevqk
      241 gmlqvmkdyl qskenke
//
LOCUS       XP_077498277             205 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109088 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498277
VERSION     XP_077498277.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642151.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..205
                     /product="uncharacterized protein LOC144109088 isoform X1"
                     /calculated_mol_wt=22342
     CDS             1..205
                     /gene="LOC144109088"
                     /coded_by="XM_077642151.1:1443..2060"
                     /db_xref="GeneID:144109088"
ORIGIN      
        1 mlkmiwgdia avvhlykryt aerrkgvtrn gaihavalvl latdmspmep llagkeqrck
       61 visilgpita sravsahnvv ynqkcpdvnp avapevvdlh gapessdalh psldnasqsh
      121 llvevrqtlk pqgapqhcrr ftgfdanifl ndaedpfmsr hligracvvr ivspvtkrda
      181 latrlalrlv mfrittssas rplql
//
LOCUS       XP_077498278             205 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109088 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498278
VERSION     XP_077498278.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642152.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..205
                     /product="uncharacterized protein LOC144109088 isoform X1"
                     /calculated_mol_wt=22342
     CDS             1..205
                     /gene="LOC144109088"
                     /coded_by="XM_077642152.1:963..1580"
                     /db_xref="GeneID:144109088"
ORIGIN      
        1 mlkmiwgdia avvhlykryt aerrkgvtrn gaihavalvl latdmspmep llagkeqrck
       61 visilgpita sravsahnvv ynqkcpdvnp avapevvdlh gapessdalh psldnasqsh
      121 llvevrqtlk pqgapqhcrr ftgfdanifl ndaedpfmsr hligracvvr ivspvtkrda
      181 latrlalrlv mfrittssas rplql
//
LOCUS       XP_077498279             205 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109088 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498279
VERSION     XP_077498279.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642153.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..205
                     /product="uncharacterized protein LOC144109088 isoform X1"
                     /calculated_mol_wt=22342
     CDS             1..205
                     /gene="LOC144109088"
                     /coded_by="XM_077642153.1:1317..1934"
                     /db_xref="GeneID:144109088"
ORIGIN      
        1 mlkmiwgdia avvhlykryt aerrkgvtrn gaihavalvl latdmspmep llagkeqrck
       61 visilgpita sravsahnvv ynqkcpdvnp avapevvdlh gapessdalh psldnasqsh
      121 llvevrqtlk pqgapqhcrr ftgfdanifl ndaedpfmsr hligracvvr ivspvtkrda
      181 latrlalrlv mfrittssas rplql
//
LOCUS       XP_077498280             205 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109088 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498280
VERSION     XP_077498280.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642154.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..205
                     /product="uncharacterized protein LOC144109088 isoform X1"
                     /calculated_mol_wt=22342
     CDS             1..205
                     /gene="LOC144109088"
                     /coded_by="XM_077642154.1:1230..1847"
                     /db_xref="GeneID:144109088"
ORIGIN      
        1 mlkmiwgdia avvhlykryt aerrkgvtrn gaihavalvl latdmspmep llagkeqrck
       61 visilgpita sravsahnvv ynqkcpdvnp avapevvdlh gapessdalh psldnasqsh
      121 llvevrqtlk pqgapqhcrr ftgfdanifl ndaedpfmsr hligracvvr ivspvtkrda
      181 latrlalrlv mfrittssas rplql
//
LOCUS       XP_077498281             205 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109088 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498281
VERSION     XP_077498281.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642155.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..205
                     /product="uncharacterized protein LOC144109088 isoform X1"
                     /calculated_mol_wt=22342
     CDS             1..205
                     /gene="LOC144109088"
                     /coded_by="XM_077642155.1:1353..1970"
                     /db_xref="GeneID:144109088"
ORIGIN      
        1 mlkmiwgdia avvhlykryt aerrkgvtrn gaihavalvl latdmspmep llagkeqrck
       61 visilgpita sravsahnvv ynqkcpdvnp avapevvdlh gapessdalh psldnasqsh
      121 llvevrqtlk pqgapqhcrr ftgfdanifl ndaedpfmsr hligracvvr ivspvtkrda
      181 latrlalrlv mfrittssas rplql
//
LOCUS       XP_077498282             205 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109088 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498282
VERSION     XP_077498282.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642156.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..205
                     /product="uncharacterized protein LOC144109088 isoform X1"
                     /calculated_mol_wt=22342
     CDS             1..205
                     /gene="LOC144109088"
                     /coded_by="XM_077642156.1:837..1454"
                     /db_xref="GeneID:144109088"
ORIGIN      
        1 mlkmiwgdia avvhlykryt aerrkgvtrn gaihavalvl latdmspmep llagkeqrck
       61 visilgpita sravsahnvv ynqkcpdvnp avapevvdlh gapessdalh psldnasqsh
      121 llvevrqtlk pqgapqhcrr ftgfdanifl ndaedpfmsr hligracvvr ivspvtkrda
      181 latrlalrlv mfrittssas rplql
//
LOCUS       XP_077498283             205 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109088 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498283
VERSION     XP_077498283.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642157.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..205
                     /product="uncharacterized protein LOC144109088 isoform X1"
                     /calculated_mol_wt=22342
     CDS             1..205
                     /gene="LOC144109088"
                     /coded_by="XM_077642157.1:750..1367"
                     /db_xref="GeneID:144109088"
ORIGIN      
        1 mlkmiwgdia avvhlykryt aerrkgvtrn gaihavalvl latdmspmep llagkeqrck
       61 visilgpita sravsahnvv ynqkcpdvnp avapevvdlh gapessdalh psldnasqsh
      121 llvevrqtlk pqgapqhcrr ftgfdanifl ndaedpfmsr hligracvvr ivspvtkrda
      181 latrlalrlv mfrittssas rplql
//
LOCUS       XP_077498284             205 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109088 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498284
VERSION     XP_077498284.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642158.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..205
                     /product="uncharacterized protein LOC144109088 isoform X1"
                     /calculated_mol_wt=22342
     CDS             1..205
                     /gene="LOC144109088"
                     /coded_by="XM_077642158.1:1284..1901"
                     /db_xref="GeneID:144109088"
ORIGIN      
        1 mlkmiwgdia avvhlykryt aerrkgvtrn gaihavalvl latdmspmep llagkeqrck
       61 visilgpita sravsahnvv ynqkcpdvnp avapevvdlh gapessdalh psldnasqsh
      121 llvevrqtlk pqgapqhcrr ftgfdanifl ndaedpfmsr hligracvvr ivspvtkrda
      181 latrlalrlv mfrittssas rplql
//
LOCUS       XP_077498285             205 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109088 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498285
VERSION     XP_077498285.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642159.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..205
                     /product="uncharacterized protein LOC144109088 isoform X1"
                     /calculated_mol_wt=22342
     CDS             1..205
                     /gene="LOC144109088"
                     /coded_by="XM_077642159.1:1138..1755"
                     /db_xref="GeneID:144109088"
ORIGIN      
        1 mlkmiwgdia avvhlykryt aerrkgvtrn gaihavalvl latdmspmep llagkeqrck
       61 visilgpita sravsahnvv ynqkcpdvnp avapevvdlh gapessdalh psldnasqsh
      121 llvevrqtlk pqgapqhcrr ftgfdanifl ndaedpfmsr hligracvvr ivspvtkrda
      181 latrlalrlv mfrittssas rplql
//
LOCUS       XP_077498286             205 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109088 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498286
VERSION     XP_077498286.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642160.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..205
                     /product="uncharacterized protein LOC144109088 isoform X1"
                     /calculated_mol_wt=22342
     CDS             1..205
                     /gene="LOC144109088"
                     /coded_by="XM_077642160.1:1407..2024"
                     /db_xref="GeneID:144109088"
ORIGIN      
        1 mlkmiwgdia avvhlykryt aerrkgvtrn gaihavalvl latdmspmep llagkeqrck
       61 visilgpita sravsahnvv ynqkcpdvnp avapevvdlh gapessdalh psldnasqsh
      121 llvevrqtlk pqgapqhcrr ftgfdanifl ndaedpfmsr hligracvvr ivspvtkrda
      181 latrlalrlv mfrittssas rplql
//
LOCUS       XP_077498287             132 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger X-chromosomal protein-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077498287
VERSION     XP_077498287.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642161.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..132
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..132
                     /product="zinc finger X-chromosomal protein-like isoform
                     X2"
                     /calculated_mol_wt=15417
     Region          28..51
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(28,31,44,51)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(68,70..71,74..75,78,92,94,98..99,102..103,106,120,
                     122,124,126..127,130..131)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          87..107
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          99..121
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          115..131
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..132
                     /gene="LOC144109088"
                     /coded_by="XM_077642161.1:517..915"
                     /db_xref="GeneID:144109088"
ORIGIN      
        1 mrkrttaglf lsakdvdivp lnslffacdk cgdlfstqyn lemhtrrkqw hsthgirrct
       61 ygtytssrkf niirherthv grreslcplc qksfsqpknl kvhmnihsge kpyecgqcgk
      121 rlrqrvharr he
//
LOCUS       XP_077498288             132 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger X-chromosomal protein-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077498288
VERSION     XP_077498288.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642162.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..132
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..132
                     /product="zinc finger X-chromosomal protein-like isoform
                     X2"
                     /calculated_mol_wt=15417
     Region          28..51
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(28,31,44,51)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(68,70..71,74..75,78,92,94,98..99,102..103,106,120,
                     122,124,126..127,130..131)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          87..107
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          99..121
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          115..131
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..132
                     /gene="LOC144109088"
                     /coded_by="XM_077642162.1:430..828"
                     /db_xref="GeneID:144109088"
ORIGIN      
        1 mrkrttaglf lsakdvdivp lnslffacdk cgdlfstqyn lemhtrrkqw hsthgirrct
       61 ygtytssrkf niirherthv grreslcplc qksfsqpknl kvhmnihsge kpyecgqcgk
      121 rlrqrvharr he
//
LOCUS       XP_077498289             132 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger X-chromosomal protein-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077498289
VERSION     XP_077498289.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642163.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..132
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..132
                     /product="zinc finger X-chromosomal protein-like isoform
                     X2"
                     /calculated_mol_wt=15417
     Region          28..51
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(28,31,44,51)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(68,70..71,74..75,78,92,94,98..99,102..103,106,120,
                     122,124,126..127,130..131)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          87..107
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          99..121
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          115..131
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..132
                     /gene="LOC144109088"
                     /coded_by="XM_077642163.1:251..649"
                     /db_xref="GeneID:144109088"
ORIGIN      
        1 mrkrttaglf lsakdvdivp lnslffacdk cgdlfstqyn lemhtrrkqw hsthgirrct
       61 ygtytssrkf niirherthv grreslcplc qksfsqpknl kvhmnihsge kpyecgqcgk
      121 rlrqrvharr he
//
LOCUS       XP_077498290             108 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger X-chromosomal protein-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077498290
VERSION     XP_077498290.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642164.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..108
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..108
                     /product="zinc finger X-chromosomal protein-like isoform
                     X3"
                     /calculated_mol_wt=12787
     Site            order(44,46..47,50..51,54,68,70,74..75,78..79,82,96,98,
                     100,102..103,106..107)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          63..83
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          75..97
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          91..107
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..108
                     /gene="LOC144109088"
                     /coded_by="XM_077642164.1:643..969"
                     /db_xref="GeneID:144109088"
ORIGIN      
        1 mrkrttaglf lsakynlemh trrkqwhsth girrctygty tssrkfniir herthvgrre
       61 slcplcqksf sqpknlkvhm nihsgekpye cgqcgkrlrq rvharrhe
//
LOCUS       XP_077498291             108 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger X-chromosomal protein-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077498291
VERSION     XP_077498291.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642165.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..108
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..108
                     /product="zinc finger X-chromosomal protein-like isoform
                     X3"
                     /calculated_mol_wt=12787
     Site            order(44,46..47,50..51,54,68,70,74..75,78..79,82,96,98,
                     100,102..103,106..107)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          63..83
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          75..97
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          91..107
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..108
                     /gene="LOC144109088"
                     /coded_by="XM_077642165.1:430..756"
                     /db_xref="GeneID:144109088"
ORIGIN      
        1 mrkrttaglf lsakynlemh trrkqwhsth girrctygty tssrkfniir herthvgrre
       61 slcplcqksf sqpknlkvhm nihsgekpye cgqcgkrlrq rvharrhe
//
LOCUS       XP_077498292             108 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger X-chromosomal protein-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077498292
VERSION     XP_077498292.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642166.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..108
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..108
                     /product="zinc finger X-chromosomal protein-like isoform
                     X3"
                     /calculated_mol_wt=12787
     Site            order(44,46..47,50..51,54,68,70,74..75,78..79,82,96,98,
                     100,102..103,106..107)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          63..83
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          75..97
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          91..107
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..108
                     /gene="LOC144109088"
                     /coded_by="XM_077642166.1:251..577"
                     /db_xref="GeneID:144109088"
ORIGIN      
        1 mrkrttaglf lsakynlemh trrkqwhsth girrctygty tssrkfniir herthvgrre
       61 slcplcqksf sqpknlkvhm nihsgekpye cgqcgkrlrq rvharrhe
//
LOCUS       XP_077498293              90 aa            linear   INV 25-NOV-2025
DEFINITION  putative transcription factor Ken isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077498293
VERSION     XP_077498293.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642167.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..90
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..90
                     /product="putative transcription factor Ken isoform X4"
                     /calculated_mol_wt=10705
     Site            order(26,28..29,32..33,36,50,52,56..57,60..61,64,78,80,82,
                     84..85,88..89)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          41..>73
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          45..65
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          73..89
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..90
                     /gene="LOC144109088"
                     /coded_by="XM_077642167.1:743..1015"
                     /db_xref="GeneID:144109088"
ORIGIN      
        1 mhtrrkqwhs thgirrctyg tytssrkfni irherthvgr reslcplcqk sfsqpknlkv
       61 hmnihsgekp yecgqcgkrl rqrvharrhe
//
LOCUS       XP_077498294              90 aa            linear   INV 25-NOV-2025
DEFINITION  putative transcription factor Ken isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077498294
VERSION     XP_077498294.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642168.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..90
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..90
                     /product="putative transcription factor Ken isoform X4"
                     /calculated_mol_wt=10705
     Site            order(26,28..29,32..33,36,50,52,56..57,60..61,64,78,80,82,
                     84..85,88..89)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          41..>73
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          45..65
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          73..89
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..90
                     /gene="LOC144109088"
                     /coded_by="XM_077642168.1:682..954"
                     /db_xref="GeneID:144109088"
ORIGIN      
        1 mhtrrkqwhs thgirrctyg tytssrkfni irherthvgr reslcplcqk sfsqpknlkv
       61 hmnihsgekp yecgqcgkrl rqrvharrhe
//
LOCUS       XP_077498295              90 aa            linear   INV 25-NOV-2025
DEFINITION  putative transcription factor Ken isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077498295
VERSION     XP_077498295.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642169.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..90
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..90
                     /product="putative transcription factor Ken isoform X4"
                     /calculated_mol_wt=10705
     Site            order(26,28..29,32..33,36,50,52,56..57,60..61,64,78,80,82,
                     84..85,88..89)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          41..>73
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          45..65
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          73..89
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..90
                     /gene="LOC144109088"
                     /coded_by="XM_077642169.1:636..908"
                     /db_xref="GeneID:144109088"
ORIGIN      
        1 mhtrrkqwhs thgirrctyg tytssrkfni irherthvgr reslcplcqk sfsqpknlkv
       61 hmnihsgekp yecgqcgkrl rqrvharrhe
//
LOCUS       XP_077498296              90 aa            linear   INV 25-NOV-2025
DEFINITION  putative transcription factor Ken isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077498296
VERSION     XP_077498296.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642170.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..90
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..90
                     /product="putative transcription factor Ken isoform X4"
                     /calculated_mol_wt=10705
     Site            order(26,28..29,32..33,36,50,52,56..57,60..61,64,78,80,82,
                     84..85,88..89)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          41..>73
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          45..65
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          73..89
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..90
                     /gene="LOC144109088"
                     /coded_by="XM_077642170.1:495..767"
                     /db_xref="GeneID:144109088"
ORIGIN      
        1 mhtrrkqwhs thgirrctyg tytssrkfni irherthvgr reslcplcqk sfsqpknlkv
       61 hmnihsgekp yecgqcgkrl rqrvharrhe
//
LOCUS       XP_077498297              90 aa            linear   INV 25-NOV-2025
DEFINITION  putative transcription factor Ken isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077498297
VERSION     XP_077498297.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642171.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..90
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..90
                     /product="putative transcription factor Ken isoform X4"
                     /calculated_mol_wt=10705
     Site            order(26,28..29,32..33,36,50,52,56..57,60..61,64,78,80,82,
                     84..85,88..89)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          41..>73
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          45..65
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          73..89
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..90
                     /gene="LOC144109088"
                     /coded_by="XM_077642171.1:469..741"
                     /db_xref="GeneID:144109088"
ORIGIN      
        1 mhtrrkqwhs thgirrctyg tytssrkfni irherthvgr reslcplcqk sfsqpknlkv
       61 hmnihsgekp yecgqcgkrl rqrvharrhe
//
LOCUS       XP_077498298              90 aa            linear   INV 25-NOV-2025
DEFINITION  putative transcription factor Ken isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077498298
VERSION     XP_077498298.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642172.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..90
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..90
                     /product="putative transcription factor Ken isoform X4"
                     /calculated_mol_wt=10705
     Site            order(26,28..29,32..33,36,50,52,56..57,60..61,64,78,80,82,
                     84..85,88..89)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          41..>73
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          45..65
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          73..89
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..90
                     /gene="LOC144109088"
                     /coded_by="XM_077642172.1:423..695"
                     /db_xref="GeneID:144109088"
ORIGIN      
        1 mhtrrkqwhs thgirrctyg tytssrkfni irherthvgr reslcplcqk sfsqpknlkv
       61 hmnihsgekp yecgqcgkrl rqrvharrhe
//
LOCUS       XP_077498299              90 aa            linear   INV 25-NOV-2025
DEFINITION  putative transcription factor Ken isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077498299
VERSION     XP_077498299.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642173.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..90
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..90
                     /product="putative transcription factor Ken isoform X4"
                     /calculated_mol_wt=10705
     Site            order(26,28..29,32..33,36,50,52,56..57,60..61,64,78,80,82,
                     84..85,88..89)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          41..>73
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          45..65
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          73..89
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..90
                     /gene="LOC144109088"
                     /coded_by="XM_077642173.1:244..516"
                     /db_xref="GeneID:144109088"
ORIGIN      
        1 mhtrrkqwhs thgirrctyg tytssrkfni irherthvgr reslcplcqk sfsqpknlkv
       61 hmnihsgekp yecgqcgkrl rqrvharrhe
//
LOCUS       XP_077498304             187 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109393 [Amblyomma americanum].
ACCESSION   XP_077498304
VERSION     XP_077498304.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642178.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="uncharacterized protein LOC144109393"
                     /calculated_mol_wt=21140
     CDS             1..187
                     /gene="LOC144109393"
                     /coded_by="XM_077642178.1:576..1139"
                     /db_xref="GeneID:144109393"
ORIGIN      
        1 mrerrkikar sgaspdelyq pkwahykslm fldtscssqp sfstlhtqee eeeterqceh
       61 wnnesadqem dfpcssqnca epslsaqqig vgtsrpgkkr rqnddeteqr kvllttavet
      121 lqrsqqrlaa ndecdafgsm mahavrqipp gpdrqlamln ahqaivkinl srhsvpveml
      181 nvlnmqe
//
LOCUS       XP_077498316             204 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109400 [Amblyomma americanum].
ACCESSION   XP_077498316
VERSION     XP_077498316.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642190.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..204
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..204
                     /product="uncharacterized protein LOC144109400"
                     /calculated_mol_wt=22657
     Region          <74..198
                     /region_name="P2RX7_C"
                     /note="P2X purinoreceptor 7 intracellular domain;
                     pfam20478"
                     /db_xref="CDD:466627"
     CDS             1..204
                     /gene="LOC144109400"
                     /coded_by="XM_077642190.1:1..615"
                     /db_xref="GeneID:144109400"
ORIGIN      
        1 madlpelqrr llersrllgf epygdtplrv pdqpssedds ggsdngcedg acgqqlavva
       61 rgttatfgip dreppepgrv rnthwcfcgr cvsmptales tccreipaat vkqpsgcite
      121 hphfytlcld evvltvalqm lldhglrveh tgryryisys qlahwlwgrl grhhrkvlpa
      181 cavcqirerf psegytgfqy aqgl
//
LOCUS       XP_077498326             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109411 [Amblyomma americanum].
ACCESSION   XP_077498326
VERSION     XP_077498326.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642200.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144109411"
                     /calculated_mol_wt=17095
     CDS             1..158
                     /gene="LOC144109411"
                     /coded_by="XM_077642200.1:100..576"
                     /db_xref="GeneID:144109411"
ORIGIN      
        1 mepllagkeq rcmvisilgp itasravsah nvvynqkcpd vnpavapevv dlhgapessd
       61 alhpsldnas qsdvlvefrq tlkpqgapqh crrftgfdan iflndaedpf msrhqiarac
      121 vvkivlpvtk raalatrlal llvmfrmtts sasrplql
//
LOCUS       XP_077498334             131 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109418 [Amblyomma americanum].
ACCESSION   XP_077498334
VERSION     XP_077498334.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642208.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..131
                     /product="uncharacterized protein LOC144109418"
                     /calculated_mol_wt=14572
     CDS             1..131
                     /gene="LOC144109418"
                     /coded_by="XM_077642208.1:382..777"
                     /db_xref="GeneID:144109418"
ORIGIN      
        1 mmrhrqsalp lltdlkvpal ivieslifag tevvldlcle alhkgrhsas favlvltrll
       61 ytvwlavlfr asdpllasgl ammrdrfama aflyaydgys kylvclprtl sylygsetct
      121 lptwcrkpsg r
//
LOCUS       XP_077498343             592 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109425 [Amblyomma americanum].
ACCESSION   XP_077498343
VERSION     XP_077498343.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642217.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..592
                     /product="uncharacterized protein LOC144109425"
                     /calculated_mol_wt=65935
     Region          4..93
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          <355..545
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..592
                     /gene="LOC144109425"
                     /coded_by="XM_077642217.1:397..2175"
                     /db_xref="GeneID:144109425"
ORIGIN      
        1 mptcfvpgct sgyrrntekr hffspptdpn llakwsraip radkvlgktc kvcdahfqeg
       61 dilksykhvi ngevieierg vwslrsdaip tifpnlplyf snrkrrkspk kratvsrdav
      121 atqsdaaedi diereqpepv qdsclhvnel veksaslrlp rqwrsaaled engrrsqivf
      181 yragleadvy tvlksvvvre dmsyvvsarg klvptlpmdl aitsladvea ivqvvqtmqa
      241 chgctssrgg tvyhkqcrvl teaekgvcik cakmtkdlkr rsglkpkkkv claapvlrkr
      301 liraaaareq kkkeisqlse klkqafstsl gevtqdlpea qkmviqaaim kqaaksprgh
      361 ryttdwlmic lllrltspkc yrtlsdmkil plpsanrlvq llkglpcgyg lnkfalgsik
      421 lhmagkpehy tygcliidev klregtefnr ssykfdgfvd ygditkvgtd qladhalvvm
      481 fnpmfaswvq piatfaakga apgwvlakvv mssvlqlqeh garvlavisd gagnnksmwt
      541 hlgisgkveh pqckiehpcl pegtflhfic dvphivkcir nhmmkhkygq as
//
LOCUS       XP_077498360             914 aa            linear   INV 25-NOV-2025
DEFINITION  heat shock 70 kDa protein 12A-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498360
VERSION     XP_077498360.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642234.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..914
                     /product="heat shock 70 kDa protein 12A-like isoform X1"
                     /calculated_mol_wt=101183
     Region          326..765
                     /region_name="ASKHA_ATPase-like"
                     /note="ATPase-like domain of the ASKHA (Acetate and Sugar
                     Kinases/Hsc70/Actin) superfamily; cl49607"
                     /db_xref="CDD:483947"
     CDS             1..914
                     /gene="LOC144109438"
                     /coded_by="XM_077642234.1:187..2931"
                     /db_xref="GeneID:144109438"
ORIGIN      
        1 mqappssnlv wtppyrrgsf ghlyplrlyr nrlqptedcp lltaylsser ttplgckqln
       61 kkdpqschag arlqeaglrq esaegavfgv latptcqrki ahlfrddlmt qrdnhsllgh
      121 raprpparsr tlcsfgrple sapathcvnp nvrvqrrvls dnfqdfcqtm leprvpaafr
      181 laskdsprrr slsslcrtsv vqealrvsss aavdtnldqd alqviqqldl siagppqpvy
      241 pkrggsgdvq ndnlieevcf pgsnkrsyaa lgaqeedqvt qaannglekp aralseagdi
      301 cvmsvdlqpg rsdtaasdgt agshfvvvai dfgttysgya fsftrdpdnv hmmrkweggd
      361 pgvvnektpt tlllnpdgef hsfgytardv yhdldaqeak rwmffekfkm alhssenlsr
      421 dteiraangr pmaalavfah alryfrdqal kelseqsatv iladdvrwvv tvpaiwrqpa
      481 kqfmraaayk agigspdfpe qliialepea asvycrklrm hqlvpeapar vhlfreaape
      541 lntepaveqr tgtrymvvdc gggtvditvh elhdgtlkel hkatggpcgs lgidreferl
      601 lrsifgadfm eqfrlkkpaa yvdlmvafea rkrnaspdkd tprnislpfs fidyykkckg
      661 atveqaikry gckevkwssq gmlrlepaam relfrptlar irehiggvlg dprlgaihyl
      721 flvggfaesq mlqkelreaf aphtrviipq gvslailrga vlfgldpmvv nvrrsrltyg
      781 vgvlnrfvhg vhppaklvvk dgiewcadvl dafvladqsv gqgdvvvrsy tpaksgqtrs
      841 iihvycserd dvrfitdpgv vrcgtlvldl sqqdapgrre iqarmvfget eikvsaldva
      901 thkcvradid flnh
//
LOCUS       XP_077498368             806 aa            linear   INV 25-NOV-2025
DEFINITION  heat shock 70 kDa protein 12A-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077498368
VERSION     XP_077498368.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642242.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..806
                     /product="heat shock 70 kDa protein 12A-like isoform X2"
                     /calculated_mol_wt=89092
     Region          218..657
                     /region_name="ASKHA_ATPase-like"
                     /note="ATPase-like domain of the ASKHA (Acetate and Sugar
                     Kinases/Hsc70/Actin) superfamily; cl49607"
                     /db_xref="CDD:483947"
     CDS             1..806
                     /gene="LOC144109438"
                     /coded_by="XM_077642242.1:358..2778"
                     /db_xref="GeneID:144109438"
ORIGIN      
        1 mtqrdnhsll ghraprppar srtlcsfgrp lesapathcv npnvrvqrrv lsdnfqdfcq
       61 tmleprvpaa frlaskdspr rrslsslcrt svvqealrvs ssaavdtnld qdalqviqql
      121 dlsiagppqp vypkrggsgd vqndnlieev cfpgsnkrsy aalgaqeedq vtqaanngle
      181 kparalseag dicvmsvdlq pgrsdtaasd gtagshfvvv aidfgttysg yafsftrdpd
      241 nvhmmrkweg gdpgvvnekt pttlllnpdg efhsfgytar dvyhdldaqe akrwmffekf
      301 kmalhssenl srdteiraan grpmaalavf ahalryfrdq alkelseqsa tviladdvrw
      361 vvtvpaiwrq pakqfmraaa ykagigspdf peqliialep eaasvycrkl rmhqlvpeap
      421 arvhlfreaa pelntepave qrtgtrymvv dcgggtvdit vhelhdgtlk elhkatggpc
      481 gslgidrefe rllrsifgad fmeqfrlkkp aayvdlmvaf earkrnaspd kdtprnislp
      541 fsfidyykkc kgatveqaik rygckevkws sqgmlrlepa amrelfrptl arirehiggv
      601 lgdprlgaih ylflvggfae sqmlqkelre afaphtrvii pqgvslailr gavlfgldpm
      661 vvnvrrsrlt ygvgvlnrfv hgvhppaklv vkdgiewcad vldafvladq svgqgdvvvr
      721 sytpaksgqt rsiihvycse rddvrfitdp gvvrcgtlvl dlsqqdapgr reiqarmvfg
      781 eteikvsald vathkcvrad idflnh
//
LOCUS       XP_077498380             194 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109453 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498380
VERSION     XP_077498380.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642254.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..194
                     /product="uncharacterized protein LOC144109453 isoform X1"
                     /calculated_mol_wt=21301
     CDS             1..194
                     /gene="LOC144109453"
                     /coded_by="XM_077642254.1:125..709"
                     /db_xref="GeneID:144109453"
ORIGIN      
        1 mgrkdaaamh gnekkhfssv kyqrmqrtsd gfidiqfqkt lsrvpkkaia laaclfiggt
       61 vliilasfif agffdpkihw shpgvlscvh rlaavccdra acilfgasvq gscgetrhqs
      121 ppaavalait aesypsvfnw pslrgpaetf tppseqrrsl rgrcrrdlcc mtclkcmktr
      181 rglrsmyfla rgsi
//
LOCUS       XP_077498390             194 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109453 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498390
VERSION     XP_077498390.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642264.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..194
                     /product="uncharacterized protein LOC144109453 isoform X1"
                     /calculated_mol_wt=21301
     CDS             1..194
                     /gene="LOC144109453"
                     /coded_by="XM_077642264.1:134..718"
                     /db_xref="GeneID:144109453"
ORIGIN      
        1 mgrkdaaamh gnekkhfssv kyqrmqrtsd gfidiqfqkt lsrvpkkaia laaclfiggt
       61 vliilasfif agffdpkihw shpgvlscvh rlaavccdra acilfgasvq gscgetrhqs
      121 ppaavalait aesypsvfnw pslrgpaetf tppseqrrsl rgrcrrdlcc mtclkcmktr
      181 rglrsmyfla rgsi
//
LOCUS       XP_077498397             194 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109453 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498397
VERSION     XP_077498397.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642271.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..194
                     /product="uncharacterized protein LOC144109453 isoform X1"
                     /calculated_mol_wt=21301
     CDS             1..194
                     /gene="LOC144109453"
                     /coded_by="XM_077642271.1:120..704"
                     /db_xref="GeneID:144109453"
ORIGIN      
        1 mgrkdaaamh gnekkhfssv kyqrmqrtsd gfidiqfqkt lsrvpkkaia laaclfiggt
       61 vliilasfif agffdpkihw shpgvlscvh rlaavccdra acilfgasvq gscgetrhqs
      121 ppaavalait aesypsvfnw pslrgpaetf tppseqrrsl rgrcrrdlcc mtclkcmktr
      181 rglrsmyfla rgsi
//
LOCUS       XP_077498406             194 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109453 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498406
VERSION     XP_077498406.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642280.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..194
                     /product="uncharacterized protein LOC144109453 isoform X1"
                     /calculated_mol_wt=21301
     CDS             1..194
                     /gene="LOC144109453"
                     /coded_by="XM_077642280.1:246..830"
                     /db_xref="GeneID:144109453"
ORIGIN      
        1 mgrkdaaamh gnekkhfssv kyqrmqrtsd gfidiqfqkt lsrvpkkaia laaclfiggt
       61 vliilasfif agffdpkihw shpgvlscvh rlaavccdra acilfgasvq gscgetrhqs
      121 ppaavalait aesypsvfnw pslrgpaetf tppseqrrsl rgrcrrdlcc mtclkcmktr
      181 rglrsmyfla rgsi
//
LOCUS       XP_077498414             194 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109453 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498414
VERSION     XP_077498414.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642288.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..194
                     /product="uncharacterized protein LOC144109453 isoform X1"
                     /calculated_mol_wt=21301
     CDS             1..194
                     /gene="LOC144109453"
                     /coded_by="XM_077642288.1:60..644"
                     /db_xref="GeneID:144109453"
ORIGIN      
        1 mgrkdaaamh gnekkhfssv kyqrmqrtsd gfidiqfqkt lsrvpkkaia laaclfiggt
       61 vliilasfif agffdpkihw shpgvlscvh rlaavccdra acilfgasvq gscgetrhqs
      121 ppaavalait aesypsvfnw pslrgpaetf tppseqrrsl rgrcrrdlcc mtclkcmktr
      181 rglrsmyfla rgsi
//
LOCUS       XP_077498423             194 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109453 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498423
VERSION     XP_077498423.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642297.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..194
                     /product="uncharacterized protein LOC144109453 isoform X1"
                     /calculated_mol_wt=21301
     CDS             1..194
                     /gene="LOC144109453"
                     /coded_by="XM_077642297.1:43..627"
                     /db_xref="GeneID:144109453"
ORIGIN      
        1 mgrkdaaamh gnekkhfssv kyqrmqrtsd gfidiqfqkt lsrvpkkaia laaclfiggt
       61 vliilasfif agffdpkihw shpgvlscvh rlaavccdra acilfgasvq gscgetrhqs
      121 ppaavalait aesypsvfnw pslrgpaetf tppseqrrsl rgrcrrdlcc mtclkcmktr
      181 rglrsmyfla rgsi
//
LOCUS       XP_077498429             194 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109453 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498429
VERSION     XP_077498429.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642303.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..194
                     /product="uncharacterized protein LOC144109453 isoform X1"
                     /calculated_mol_wt=21301
     CDS             1..194
                     /gene="LOC144109453"
                     /coded_by="XM_077642303.1:133..717"
                     /db_xref="GeneID:144109453"
ORIGIN      
        1 mgrkdaaamh gnekkhfssv kyqrmqrtsd gfidiqfqkt lsrvpkkaia laaclfiggt
       61 vliilasfif agffdpkihw shpgvlscvh rlaavccdra acilfgasvq gscgetrhqs
      121 ppaavalait aesypsvfnw pslrgpaetf tppseqrrsl rgrcrrdlcc mtclkcmktr
      181 rglrsmyfla rgsi
//
LOCUS       XP_077498435             194 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109453 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498435
VERSION     XP_077498435.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642309.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..194
                     /product="uncharacterized protein LOC144109453 isoform X1"
                     /calculated_mol_wt=21301
     CDS             1..194
                     /gene="LOC144109453"
                     /coded_by="XM_077642309.1:42..626"
                     /db_xref="GeneID:144109453"
ORIGIN      
        1 mgrkdaaamh gnekkhfssv kyqrmqrtsd gfidiqfqkt lsrvpkkaia laaclfiggt
       61 vliilasfif agffdpkihw shpgvlscvh rlaavccdra acilfgasvq gscgetrhqs
      121 ppaavalait aesypsvfnw pslrgpaetf tppseqrrsl rgrcrrdlcc mtclkcmktr
      181 rglrsmyfla rgsi
//
LOCUS       XP_077498441             122 aa            linear   INV 25-NOV-2025
DEFINITION  transmembrane protein 230 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077498441
VERSION     XP_077498441.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642315.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..122
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..122
                     /product="transmembrane protein 230 isoform X2"
                     /calculated_mol_wt=13657
     Region          <47..121
                     /region_name="TMEM_230_134"
                     /note="Transmembrane proteins 230/134; pfam05915"
                     /db_xref="CDD:399128"
     CDS             1..122
                     /gene="LOC144109453"
                     /coded_by="XM_077642315.1:125..493"
                     /db_xref="GeneID:144109453"
ORIGIN      
        1 mgrkdaaamh gnekkhfssv kyqrmqrtsd gfidiqfqkt lsrvpkkaia laaclfiggt
       61 vliilasfif agffdpkydd sswpvlvlga lmfipgayhv riayyaykey pgftfddipd
      121 fd
//
LOCUS       XP_077498447             122 aa            linear   INV 25-NOV-2025
DEFINITION  transmembrane protein 230 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077498447
VERSION     XP_077498447.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642321.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..122
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..122
                     /product="transmembrane protein 230 isoform X2"
                     /calculated_mol_wt=13657
     Region          <47..121
                     /region_name="TMEM_230_134"
                     /note="Transmembrane proteins 230/134; pfam05915"
                     /db_xref="CDD:399128"
     CDS             1..122
                     /gene="LOC144109453"
                     /coded_by="XM_077642321.1:223..591"
                     /db_xref="GeneID:144109453"
ORIGIN      
        1 mgrkdaaamh gnekkhfssv kyqrmqrtsd gfidiqfqkt lsrvpkkaia laaclfiggt
       61 vliilasfif agffdpkydd sswpvlvlga lmfipgayhv riayyaykey pgftfddipd
      121 fd
//
LOCUS       XP_077498458             342 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger protein 862-like [Amblyomma americanum].
ACCESSION   XP_077498458
VERSION     XP_077498458.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642332.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..342
                     /product="zinc finger protein 862-like"
                     /calculated_mol_wt=38646
     Region          <130..234
                     /region_name="DUF4371"
                     /note="Domain of unknown function (DUF4371); cl46273"
                     /db_xref="CDD:480613"
     Region          <230..>337
                     /region_name="Periplasmic_Binding_Protein_type1"
                     /note="Type 1 periplasmic binding fold superfamily;
                     cl10011"
                     /db_xref="CDD:471960"
     CDS             1..342
                     /gene="LOC144109502"
                     /coded_by="XM_077642332.1:716..1744"
                     /db_xref="GeneID:144109502"
ORIGIN      
        1 mgkdarakrt freewrkdkk lsgwissrdg gttahckycn cnirphysdl lkhaetkkhr
       61 ecavipssqq klpqllasss rtyyekarre lrialytavh tsinavdelg eilhsefndf
      121 dlhrtkctai itnvlfpyft enmdkqlngs syslmvdest dvsttkqlcm vvrflsleen
      181 riattlldlv elsdgtaetl hdtvlktldk hglsiknclg lctdganamc gkhnslfsrl
      241 sednkdlili kcschsldlv asksmeaips avehlvrety nyfahsscrq daykrlyasm
      301 tvedesanpp pkilslsqtr wlaiadsier ilaqyqslee ff
//
LOCUS       XP_077498467             335 aa            linear   INV 25-NOV-2025
DEFINITION  vesicular integral-membrane protein VIP36 [Amblyomma americanum].
ACCESSION   XP_077498467
VERSION     XP_077498467.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642341.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..335
                     /product="vesicular integral-membrane protein VIP36"
                     /calculated_mol_wt=37525
     Region          37..284
                     /region_name="lectin_VIP36_VIPL"
                     /note="VIP36 and VIPL type 1 transmembrane proteins,
                     lectin domain; cd06901"
                     /db_xref="CDD:173889"
     Site            order(113,146,148,173,243..245)
                     /site_type="other"
                     /note="carbohydrate binding site [chemical binding]"
                     /db_xref="CDD:173889"
     Site            order(144,148,173,176)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:173889"
     CDS             1..335
                     /gene="LOC144109514"
                     /coded_by="XM_077642341.1:66..1073"
                     /db_xref="GeneID:144109514"
ORIGIN      
        1 mapleimrrl lclflfgiif rvkqnlfata ewntndymkk ehslvkpyqg agmtipnwdf
       61 lghtmvtssy irltpdqqsa kgaiwnnmpc rsknwemhvh fkvhgtgkdl fgdgfaiwya
      121 kealelgpvf gskdkfsglg iffdtyanqn gphnhghpyi samvnngtls ydhdrdgtht
      181 elagceakfr nvdhdthvvi ryendvltva tdiegknawk ecftvkgvql pthyyfgasa
      241 vtgdlsdnhd iismklyeid gpgekqgdtq drskivpaas ffapprdhvd dpppamsgtk
      301 fllivlcail gialcivvgl vlfqkqqets rkrfy
//
LOCUS       XP_077498477             103 aa            linear   INV 25-NOV-2025
DEFINITION  BLOC-1 related complex subunit 7 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077498477
VERSION     XP_077498477.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642351.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..103
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..103
                     /product="BLOC-1 related complex subunit 7 isoform X1"
                     /calculated_mol_wt=11443
     Region          2..101
                     /region_name="BORCS7"
                     /note="BLOC-1-related complex sub-unit 7; pfam16088"
                     /db_xref="CDD:465012"
     CDS             1..103
                     /gene="BORCS7"
                     /coded_by="XM_077642351.1:219..530"
                     /db_xref="GeneID:144109523"
ORIGIN      
        1 mssvssarsl fveskerlcd riqvnvnsvg slarqivkgs rtsetlmhta rnfalqehal
       61 hnsdvnlckm qilvtnlqlq adaiqkcaqh ieavkeqlhr mqc
//
LOCUS       XP_077498484              96 aa            linear   INV 25-NOV-2025
DEFINITION  BLOC-1 related complex subunit 7 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077498484
VERSION     XP_077498484.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642358.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..96
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..96
                     /product="BLOC-1 related complex subunit 7 isoform X2"
                     /calculated_mol_wt=10722
     Region          2..94
                     /region_name="BORCS7"
                     /note="BLOC-1-related complex sub-unit 7; pfam16088"
                     /db_xref="CDD:465012"
     CDS             1..96
                     /gene="BORCS7"
                     /coded_by="XM_077642358.1:219..509"
                     /db_xref="GeneID:144109523"
ORIGIN      
        1 mssvssarsl fveskerlcd riqvnvnsvg slarqivkgs rtsetlmhta rnfalqehal
       61 hnsdvnlckm qilvtnlqlh aqhieavkeq lhrmqc
//
LOCUS       XP_077498494             101 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109540 [Amblyomma americanum].
ACCESSION   XP_077498494
VERSION     XP_077498494.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642368.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..101
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..101
                     /product="uncharacterized protein LOC144109540"
                     /calculated_mol_wt=11314
     CDS             1..101
                     /gene="LOC144109540"
                     /coded_by="XM_077642368.1:933..1238"
                     /db_xref="GeneID:144109540"
ORIGIN      
        1 maprlwllpr lrlqlrdlcl lpllpwpwse ssslnqrglr arlrlrrwpf wgvpssssfl
       61 dvdslsplrp phlarrmrta vgapapaaph vtsrgaqgvp r
//
LOCUS       XP_077498501             101 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109540 [Amblyomma americanum].
ACCESSION   XP_077498501
VERSION     XP_077498501.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642375.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..101
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..101
                     /product="uncharacterized protein LOC144109540"
                     /calculated_mol_wt=11314
     CDS             1..101
                     /gene="LOC144109540"
                     /coded_by="XM_077642375.1:234..539"
                     /db_xref="GeneID:144109540"
ORIGIN      
        1 maprlwllpr lrlqlrdlcl lpllpwpwse ssslnqrglr arlrlrrwpf wgvpssssfl
       61 dvdslsplrp phlarrmrta vgapapaaph vtsrgaqgvp r
//
LOCUS       XP_077498527             360 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109578 [Amblyomma americanum].
ACCESSION   XP_077498527
VERSION     XP_077498527.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642401.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..360
                     /product="uncharacterized protein LOC144109578"
                     /calculated_mol_wt=40562
     Region          5..65
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..360
                     /gene="LOC144109578"
                     /coded_by="XM_077642401.1:1253..2335"
                     /db_xref="GeneID:144109578"
ORIGIN      
        1 mpgsccapnc ksnydggpsv rmhrfptdpt mraawttavp rgdflptkyt vlcekhflpt
       61 dylkttaytd iktgkvvevp lktirvnqta laslfpkcsa ylsrpqasvr eapeqkrirl
      121 edasmqeail lskqtqaeee aknringfsd fldalpslqn snfwtiinkd gkvfldltld
      181 sapavrssvi vssdlrvevf fgetrlskcg evlvpeklcd lrdlkrvlqm iedshrnist
      241 epsekirhlq lvatlldevs dehsacklqe whfevlkffk sqvdlvlnna nryppdflvf
      301 asllftisph aykflrssmk lklphpdtvr rlcssydasp ateqqesflc eagcehiqra
//
LOCUS       XP_077498546             379 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109600 [Amblyomma americanum].
ACCESSION   XP_077498546
VERSION     XP_077498546.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642420.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..379
                     /product="uncharacterized protein LOC144109600"
                     /calculated_mol_wt=42705
     Region          127..350
                     /region_name="transpos_IS1595"
                     /note="IS1595 family transposase; NF033547"
                     /db_xref="CDD:468078"
     CDS             1..379
                     /gene="LOC144109600"
                     /coded_by="XM_077642420.1:3169..4308"
                     /db_xref="GeneID:144109600"
ORIGIN      
        1 masaridpsl gevhlairsf eseqaflrel kltpestlar psnnqrgrpa tsefwgvckd
       61 lgfnpkspsc egevvtfnrk sakgkrpsfr cnkcrrqlsq lngtavwqga qgdgsffack
      121 drlgrpnvkl atreiiwity cmakgltsaq trelvlkdfk lrndarsdwr nyirevleqe
      181 lqarppmggv gqevqidetl lrgkrkanrg rlltgdavpl rhrnnyggva dkgpwvfgmv
      241 wsqtgelrlf qvekrdaaml gpiiaanimp gttvysdewa ayncipslqd gngaplnldw
      301 htvnhttnfv dpltgantqr iesawqkvkr nlvrngqkts ravlqshlsw qwwqsingrt
      361 kchdpflrlm eaiarrypl
//
LOCUS       XP_077498553             379 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109600 [Amblyomma americanum].
ACCESSION   XP_077498553
VERSION     XP_077498553.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642427.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..379
                     /product="uncharacterized protein LOC144109600"
                     /calculated_mol_wt=42705
     Region          127..350
                     /region_name="transpos_IS1595"
                     /note="IS1595 family transposase; NF033547"
                     /db_xref="CDD:468078"
     CDS             1..379
                     /gene="LOC144109600"
                     /coded_by="XM_077642427.1:3238..4377"
                     /db_xref="GeneID:144109600"
ORIGIN      
        1 masaridpsl gevhlairsf eseqaflrel kltpestlar psnnqrgrpa tsefwgvckd
       61 lgfnpkspsc egevvtfnrk sakgkrpsfr cnkcrrqlsq lngtavwqga qgdgsffack
      121 drlgrpnvkl atreiiwity cmakgltsaq trelvlkdfk lrndarsdwr nyirevleqe
      181 lqarppmggv gqevqidetl lrgkrkanrg rlltgdavpl rhrnnyggva dkgpwvfgmv
      241 wsqtgelrlf qvekrdaaml gpiiaanimp gttvysdewa ayncipslqd gngaplnldw
      301 htvnhttnfv dpltgantqr iesawqkvkr nlvrngqkts ravlqshlsw qwwqsingrt
      361 kchdpflrlm eaiarrypl
//
LOCUS       XP_077498561             379 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109600 [Amblyomma americanum].
ACCESSION   XP_077498561
VERSION     XP_077498561.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642435.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..379
                     /product="uncharacterized protein LOC144109600"
                     /calculated_mol_wt=42705
     Region          127..350
                     /region_name="transpos_IS1595"
                     /note="IS1595 family transposase; NF033547"
                     /db_xref="CDD:468078"
     CDS             1..379
                     /gene="LOC144109600"
                     /coded_by="XM_077642435.1:4014..5153"
                     /db_xref="GeneID:144109600"
ORIGIN      
        1 masaridpsl gevhlairsf eseqaflrel kltpestlar psnnqrgrpa tsefwgvckd
       61 lgfnpkspsc egevvtfnrk sakgkrpsfr cnkcrrqlsq lngtavwqga qgdgsffack
      121 drlgrpnvkl atreiiwity cmakgltsaq trelvlkdfk lrndarsdwr nyirevleqe
      181 lqarppmggv gqevqidetl lrgkrkanrg rlltgdavpl rhrnnyggva dkgpwvfgmv
      241 wsqtgelrlf qvekrdaaml gpiiaanimp gttvysdewa ayncipslqd gngaplnldw
      301 htvnhttnfv dpltgantqr iesawqkvkr nlvrngqkts ravlqshlsw qwwqsingrt
      361 kchdpflrlm eaiarrypl
//
LOCUS       XP_077498571             360 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109623 [Amblyomma americanum].
ACCESSION   XP_077498571
VERSION     XP_077498571.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642445.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..360
                     /product="uncharacterized protein LOC144109623"
                     /calculated_mol_wt=40562
     Region          5..65
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..360
                     /gene="LOC144109623"
                     /coded_by="XM_077642445.1:2051..3133"
                     /db_xref="GeneID:144109623"
ORIGIN      
        1 mpgsccapnc ksnydggpsv rmhrfptdpt mraawttavp rgdflptkyt vlcekhflpt
       61 dylkttaytd iktgkvvevp lktirvnqta laslfpkcsa ylsrpqasvr eapeqkrirl
      121 edasmqeail lskqtqaeee aknringfsd fldalpslqn snfwtiinkd gkvfldltld
      181 sapavrssvi vssdlrvevf fgetrlskcg evlvpeklcd lrdlkrvlqm iedshrnist
      241 epsekirhlq lvatlldevs dehsacklqe whfevlkffk sqvdlvlnna nryppdflvf
      301 asllftisph aykflrssmk lklphpdtvr rlcssydasp ateqqesflc eagcehiqra
//
LOCUS       XP_077498581             226 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109634 [Amblyomma americanum].
ACCESSION   XP_077498581
VERSION     XP_077498581.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642455.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..226
                     /product="uncharacterized protein LOC144109634"
                     /calculated_mol_wt=24955
     Region          21..109
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..226
                     /gene="LOC144109634"
                     /coded_by="XM_077642455.1:80..760"
                     /db_xref="GeneID:144109634"
ORIGIN      
        1 maatmsveps amaseassqk rvnwptvtte aliriwednl salrsntrna riynamaell
       61 naglpsgegp ytakqvrqkl enlnkqyrkl rrsgsstgsk gvqwpfywql hsflgtlpvn
      121 esdlmqesve mpkdpahtgq eivfeyednl mpvpatipct leegndstag sepagreqgt
      181 sgercgksnl rrkrpsssll relmamhqqa eeraakaake sselrk
//
LOCUS       XP_077498601             536 aa            linear   INV 25-NOV-2025
DEFINITION  cyclin-dependent kinase 14-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077498601
VERSION     XP_077498601.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642475.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..536
                     /product="cyclin-dependent kinase 14-like isoform X1"
                     /calculated_mol_wt=60009
     Region          207..492
                     /region_name="STKc_PCTAIRE_like"
                     /note="Catalytic domain of PCTAIRE-like Serine/Threonine
                     Kinases; cd07844"
                     /db_xref="CDD:270835"
     Site            order(214..217,222,235,237,253,267,283..286,289,291..292,
                     329,331,333..334,336,347,350,362,364..367,369,407..408)
                     /site_type="active"
                     /db_xref="CDD:270835"
     Site            order(214..217,222,235,237,267,283..286,289,292,333..334,
                     336,347)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270835"
     Site            order(245..247,249,252..253,255..256,259..260,272,274,279,
                     318,321..324,352,354..357,361..362,478,482..484)
                     /site_type="other"
                     /note="putative CDK/cyclin interface [polypeptide
                     binding]"
                     /db_xref="CDD:270835"
     Site            order(253,291,329,331,350,362,364..367,369,407..408)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270835"
     Site            346..369
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270835"
     CDS             1..536
                     /gene="LOC144109669"
                     /coded_by="XM_077642475.1:129..1739"
                     /db_xref="GeneID:144109669"
ORIGIN      
        1 mslsvssknw kpstkedkdr rlsriqrlrr rlscsfgrls lskevilpdg enenvavtrv
       61 tmngfekten veegaldnar sstpnsnvag repngnlspv sedkvlsepp aplppprtss
      121 shsylnkvck maarteaplg kshwprpaph sssfdectsm eyqqvsavrk parpkseill
      181 sqekgsktpr cpsmtfegnm tfgrieayvr leqlgegsya tvyrgysnlm gkvvalkeir
      241 lqpeegtpft aireasllrg lkhanivtlh diihtkdtlt fvfeyvhtdl sqylekhpgg
      301 lnpknvklfl fqllrglsyc herlilhrdl kpqnlliseq gelkladfgl araksvpsrt
      361 yshevvtlwy rppdvllgst dystsldmwg vgcifiemit gaaafpgvkd ttdqldkiwr
      421 ilgtptedtw egvsryknyk lykfgmypgq plkqafpkla etlqadeian rflqlqpqnr
      481 isaadalrhs yfadmppkvy dlpdqasift vpgcklspet ynlpmsvika aaklrc
//
LOCUS       XP_077498606             529 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial sodium/calcium exchanger protein-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077498606
VERSION     XP_077498606.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642480.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..529
                     /product="mitochondrial sodium/calcium exchanger
                     protein-like isoform X2"
                     /calculated_mol_wt=56220
     Region          2..454
                     /region_name="PLN03151"
                     /note="cation/calcium exchanger; Provisional"
                     /db_xref="CDD:215604"
     CDS             1..529
                     /gene="LOC144109669"
                     /coded_by="XM_077642480.1:110..1699"
                     /db_xref="GeneID:144109669"
ORIGIN      
        1 mascarvhdl ngtalqcafv ratadcgand awlaytafay cgplapylpl aaealwlall
       61 flvlgttadd flcpalvvvs rtmrlsesva gvtllafgng apdiisslag veqsrpalvv
      121 gellgagtfv tavvagtvfl lcrfslepap flrdavfylg asfwtfwlfy agavtlghav
      181 gflalygsyi avvlaghilr areeqqrrls vassgrrsst gsmhrhheha iaafmqashe
      241 aerrvsterv pllpppadpw aeflaqlvpw dpaewaersv agkvydvvaf plrfalvltv
      301 pvvdyadard nwcrplntlh citapvfava vlgfevgnaw alaalagaav allvwfsssf
      361 esapryhfaf gyagfalsvl wiygvarell gllrtlglva avsdavlglt vlawgnslgd
      421 lvtnvavarq gypsmamaac fggpllnllm gvglpytvrl aplgfgarlp ltltplvctl
      481 ysalvaslll slyatlfsry qasrihgaal lalyalflav altvevkfs
//
LOCUS       XP_077498611             529 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial sodium/calcium exchanger protein-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077498611
VERSION     XP_077498611.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642485.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..529
                     /product="mitochondrial sodium/calcium exchanger
                     protein-like isoform X2"
                     /calculated_mol_wt=56220
     Region          2..454
                     /region_name="PLN03151"
                     /note="cation/calcium exchanger; Provisional"
                     /db_xref="CDD:215604"
     CDS             1..529
                     /gene="LOC144109669"
                     /coded_by="XM_077642485.1:111..1700"
                     /db_xref="GeneID:144109669"
ORIGIN      
        1 mascarvhdl ngtalqcafv ratadcgand awlaytafay cgplapylpl aaealwlall
       61 flvlgttadd flcpalvvvs rtmrlsesva gvtllafgng apdiisslag veqsrpalvv
      121 gellgagtfv tavvagtvfl lcrfslepap flrdavfylg asfwtfwlfy agavtlghav
      181 gflalygsyi avvlaghilr areeqqrrls vassgrrsst gsmhrhheha iaafmqashe
      241 aerrvsterv pllpppadpw aeflaqlvpw dpaewaersv agkvydvvaf plrfalvltv
      301 pvvdyadard nwcrplntlh citapvfava vlgfevgnaw alaalagaav allvwfsssf
      361 esapryhfaf gyagfalsvl wiygvarell gllrtlglva avsdavlglt vlawgnslgd
      421 lvtnvavarq gypsmamaac fggpllnllm gvglpytvrl aplgfgarlp ltltplvctl
      481 ysalvaslll slyatlfsry qasrihgaal lalyalflav altvevkfs
//
LOCUS       XP_077498617             522 aa            linear   INV 25-NOV-2025
DEFINITION  cyclin-dependent kinase 14-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077498617
VERSION     XP_077498617.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642491.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..522
                     /product="cyclin-dependent kinase 14-like isoform X3"
                     /calculated_mol_wt=58534
     Region          193..478
                     /region_name="STKc_PCTAIRE_like"
                     /note="Catalytic domain of PCTAIRE-like Serine/Threonine
                     Kinases; cd07844"
                     /db_xref="CDD:270835"
     Site            order(200..203,208,221,223,239,253,269..272,275,277..278,
                     315,317,319..320,322,333,336,348,350..353,355,393..394)
                     /site_type="active"
                     /db_xref="CDD:270835"
     Site            order(200..203,208,221,223,253,269..272,275,278,319..320,
                     322,333)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270835"
     Site            order(231..233,235,238..239,241..242,245..246,258,260,265,
                     304,307..310,338,340..343,347..348,464,468..470)
                     /site_type="other"
                     /note="putative CDK/cyclin interface [polypeptide
                     binding]"
                     /db_xref="CDD:270835"
     Site            order(239,277,315,317,336,348,350..353,355,393..394)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270835"
     Site            332..355
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270835"
     CDS             1..522
                     /gene="LOC144109669"
                     /coded_by="XM_077642491.1:430..1998"
                     /db_xref="GeneID:144109669"
ORIGIN      
        1 mhykdrrlsr iqrlrrrlsc sfgrlslske vilpdgenen vavtrvtmng fektenveeg
       61 aldnarsstp nsnvagrepn gnlspvsedk vlseppaplp pprtssshsy lnkvckmaar
      121 teaplgkshw prpaphsssf dectsmeyqq vsavrkparp kseillsqek gsktprcpsm
      181 tfegnmtfgr ieayvrleql gegsyatvyr gysnlmgkvv alkeirlqpe egtpftaire
      241 asllrglkha nivtlhdiih tkdtltfvfe yvhtdlsqyl ekhpgglnpk nvklflfqll
      301 rglsycherl ilhrdlkpqn lliseqgelk ladfglarak svpsrtyshe vvtlwyrppd
      361 vllgstdyst sldmwgvgci fiemitgaaa fpgvkdttdq ldkiwrilgt ptedtwegvs
      421 ryknyklykf gmypgqplkq afpklaetlq adeianrflq lqpqnrisaa dalrhsyfad
      481 mppkvydlpd qasiftvpgc klspetynlp msvikaaakl rc
//
LOCUS       XP_077498623             522 aa            linear   INV 25-NOV-2025
DEFINITION  cyclin-dependent kinase 14-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077498623
VERSION     XP_077498623.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642497.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..522
                     /product="cyclin-dependent kinase 14-like isoform X3"
                     /calculated_mol_wt=58534
     Region          193..478
                     /region_name="STKc_PCTAIRE_like"
                     /note="Catalytic domain of PCTAIRE-like Serine/Threonine
                     Kinases; cd07844"
                     /db_xref="CDD:270835"
     Site            order(200..203,208,221,223,239,253,269..272,275,277..278,
                     315,317,319..320,322,333,336,348,350..353,355,393..394)
                     /site_type="active"
                     /db_xref="CDD:270835"
     Site            order(200..203,208,221,223,253,269..272,275,278,319..320,
                     322,333)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270835"
     Site            order(231..233,235,238..239,241..242,245..246,258,260,265,
                     304,307..310,338,340..343,347..348,464,468..470)
                     /site_type="other"
                     /note="putative CDK/cyclin interface [polypeptide
                     binding]"
                     /db_xref="CDD:270835"
     Site            order(239,277,315,317,336,348,350..353,355,393..394)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270835"
     Site            332..355
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270835"
     CDS             1..522
                     /gene="LOC144109669"
                     /coded_by="XM_077642497.1:649..2217"
                     /db_xref="GeneID:144109669"
ORIGIN      
        1 mhykdrrlsr iqrlrrrlsc sfgrlslske vilpdgenen vavtrvtmng fektenveeg
       61 aldnarsstp nsnvagrepn gnlspvsedk vlseppaplp pprtssshsy lnkvckmaar
      121 teaplgkshw prpaphsssf dectsmeyqq vsavrkparp kseillsqek gsktprcpsm
      181 tfegnmtfgr ieayvrleql gegsyatvyr gysnlmgkvv alkeirlqpe egtpftaire
      241 asllrglkha nivtlhdiih tkdtltfvfe yvhtdlsqyl ekhpgglnpk nvklflfqll
      301 rglsycherl ilhrdlkpqn lliseqgelk ladfglarak svpsrtyshe vvtlwyrppd
      361 vllgstdyst sldmwgvgci fiemitgaaa fpgvkdttdq ldkiwrilgt ptedtwegvs
      421 ryknyklykf gmypgqplkq afpklaetlq adeianrflq lqpqnrisaa dalrhsyfad
      481 mppkvydlpd qasiftvpgc klspetynlp msvikaaakl rc
//
LOCUS       XP_077498629             522 aa            linear   INV 25-NOV-2025
DEFINITION  cyclin-dependent kinase 14-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077498629
VERSION     XP_077498629.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642503.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..522
                     /product="cyclin-dependent kinase 14-like isoform X3"
                     /calculated_mol_wt=58534
     Region          193..478
                     /region_name="STKc_PCTAIRE_like"
                     /note="Catalytic domain of PCTAIRE-like Serine/Threonine
                     Kinases; cd07844"
                     /db_xref="CDD:270835"
     Site            order(200..203,208,221,223,239,253,269..272,275,277..278,
                     315,317,319..320,322,333,336,348,350..353,355,393..394)
                     /site_type="active"
                     /db_xref="CDD:270835"
     Site            order(200..203,208,221,223,253,269..272,275,278,319..320,
                     322,333)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270835"
     Site            order(231..233,235,238..239,241..242,245..246,258,260,265,
                     304,307..310,338,340..343,347..348,464,468..470)
                     /site_type="other"
                     /note="putative CDK/cyclin interface [polypeptide
                     binding]"
                     /db_xref="CDD:270835"
     Site            order(239,277,315,317,336,348,350..353,355,393..394)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270835"
     Site            332..355
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270835"
     CDS             1..522
                     /gene="LOC144109669"
                     /coded_by="XM_077642503.1:332..1900"
                     /db_xref="GeneID:144109669"
ORIGIN      
        1 mhykdrrlsr iqrlrrrlsc sfgrlslske vilpdgenen vavtrvtmng fektenveeg
       61 aldnarsstp nsnvagrepn gnlspvsedk vlseppaplp pprtssshsy lnkvckmaar
      121 teaplgkshw prpaphsssf dectsmeyqq vsavrkparp kseillsqek gsktprcpsm
      181 tfegnmtfgr ieayvrleql gegsyatvyr gysnlmgkvv alkeirlqpe egtpftaire
      241 asllrglkha nivtlhdiih tkdtltfvfe yvhtdlsqyl ekhpgglnpk nvklflfqll
      301 rglsycherl ilhrdlkpqn lliseqgelk ladfglarak svpsrtyshe vvtlwyrppd
      361 vllgstdyst sldmwgvgci fiemitgaaa fpgvkdttdq ldkiwrilgt ptedtwegvs
      421 ryknyklykf gmypgqplkq afpklaetlq adeianrflq lqpqnrisaa dalrhsyfad
      481 mppkvydlpd qasiftvpgc klspetynlp msvikaaakl rc
//
LOCUS       XP_077498634             522 aa            linear   INV 25-NOV-2025
DEFINITION  cyclin-dependent kinase 14-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077498634
VERSION     XP_077498634.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642508.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..522
                     /product="cyclin-dependent kinase 14-like isoform X3"
                     /calculated_mol_wt=58534
     Region          193..478
                     /region_name="STKc_PCTAIRE_like"
                     /note="Catalytic domain of PCTAIRE-like Serine/Threonine
                     Kinases; cd07844"
                     /db_xref="CDD:270835"
     Site            order(200..203,208,221,223,239,253,269..272,275,277..278,
                     315,317,319..320,322,333,336,348,350..353,355,393..394)
                     /site_type="active"
                     /db_xref="CDD:270835"
     Site            order(200..203,208,221,223,253,269..272,275,278,319..320,
                     322,333)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270835"
     Site            order(231..233,235,238..239,241..242,245..246,258,260,265,
                     304,307..310,338,340..343,347..348,464,468..470)
                     /site_type="other"
                     /note="putative CDK/cyclin interface [polypeptide
                     binding]"
                     /db_xref="CDD:270835"
     Site            order(239,277,315,317,336,348,350..353,355,393..394)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270835"
     Site            332..355
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270835"
     CDS             1..522
                     /gene="LOC144109669"
                     /coded_by="XM_077642508.1:550..2118"
                     /db_xref="GeneID:144109669"
ORIGIN      
        1 mhykdrrlsr iqrlrrrlsc sfgrlslske vilpdgenen vavtrvtmng fektenveeg
       61 aldnarsstp nsnvagrepn gnlspvsedk vlseppaplp pprtssshsy lnkvckmaar
      121 teaplgkshw prpaphsssf dectsmeyqq vsavrkparp kseillsqek gsktprcpsm
      181 tfegnmtfgr ieayvrleql gegsyatvyr gysnlmgkvv alkeirlqpe egtpftaire
      241 asllrglkha nivtlhdiih tkdtltfvfe yvhtdlsqyl ekhpgglnpk nvklflfqll
      301 rglsycherl ilhrdlkpqn lliseqgelk ladfglarak svpsrtyshe vvtlwyrppd
      361 vllgstdyst sldmwgvgci fiemitgaaa fpgvkdttdq ldkiwrilgt ptedtwegvs
      421 ryknyklykf gmypgqplkq afpklaetlq adeianrflq lqpqnrisaa dalrhsyfad
      481 mppkvydlpd qasiftvpgc klspetynlp msvikaaakl rc
//
LOCUS       XP_077498638             475 aa            linear   INV 25-NOV-2025
DEFINITION  cyclin-dependent kinase 14-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077498638
VERSION     XP_077498638.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642512.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..475
                     /product="cyclin-dependent kinase 14-like isoform X4"
                     /calculated_mol_wt=52995
     Region          146..431
                     /region_name="STKc_PCTAIRE_like"
                     /note="Catalytic domain of PCTAIRE-like Serine/Threonine
                     Kinases; cd07844"
                     /db_xref="CDD:270835"
     Site            order(153..156,161,174,176,192,206,222..225,228,230..231,
                     268,270,272..273,275,286,289,301,303..306,308,346..347)
                     /site_type="active"
                     /db_xref="CDD:270835"
     Site            order(153..156,161,174,176,206,222..225,228,231,272..273,
                     275,286)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270835"
     Site            order(184..186,188,191..192,194..195,198..199,211,213,218,
                     257,260..263,291,293..296,300..301,417,421..423)
                     /site_type="other"
                     /note="putative CDK/cyclin interface [polypeptide
                     binding]"
                     /db_xref="CDD:270835"
     Site            order(192,230,268,270,289,301,303..306,308,346..347)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270835"
     Site            285..308
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270835"
     CDS             1..475
                     /gene="LOC144109669"
                     /coded_by="XM_077642512.1:628..2055"
                     /db_xref="GeneID:144109669"
ORIGIN      
        1 mngfektenv eegaldnars stpnsnvagr epngnlspvs edkvlseppa plppprtsss
       61 hsylnkvckm aarteaplgk shwprpaphs ssfdectsme yqqvsavrkp arpkseills
      121 qekgsktprc psmtfegnmt fgrieayvrl eqlgegsyat vyrgysnlmg kvvalkeirl
      181 qpeegtpfta ireasllrgl khanivtlhd iihtkdtltf vfeyvhtdls qylekhpggl
      241 npknvklflf qllrglsych erlilhrdlk pqnlliseqg elkladfgla raksvpsrty
      301 shevvtlwyr ppdvllgstd ystsldmwgv gcifiemitg aaafpgvkdt tdqldkiwri
      361 lgtptedtwe gvsryknykl ykfgmypgqp lkqafpklae tlqadeianr flqlqpqnri
      421 saadalrhsy fadmppkvyd lpdqasiftv pgcklspety nlpmsvikaa aklrc
//
LOCUS       XP_077498642             475 aa            linear   INV 25-NOV-2025
DEFINITION  cyclin-dependent kinase 14-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077498642
VERSION     XP_077498642.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642516.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..475
                     /product="cyclin-dependent kinase 14-like isoform X4"
                     /calculated_mol_wt=52995
     Region          146..431
                     /region_name="STKc_PCTAIRE_like"
                     /note="Catalytic domain of PCTAIRE-like Serine/Threonine
                     Kinases; cd07844"
                     /db_xref="CDD:270835"
     Site            order(153..156,161,174,176,192,206,222..225,228,230..231,
                     268,270,272..273,275,286,289,301,303..306,308,346..347)
                     /site_type="active"
                     /db_xref="CDD:270835"
     Site            order(153..156,161,174,176,206,222..225,228,231,272..273,
                     275,286)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270835"
     Site            order(184..186,188,191..192,194..195,198..199,211,213,218,
                     257,260..263,291,293..296,300..301,417,421..423)
                     /site_type="other"
                     /note="putative CDK/cyclin interface [polypeptide
                     binding]"
                     /db_xref="CDD:270835"
     Site            order(192,230,268,270,289,301,303..306,308,346..347)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270835"
     Site            285..308
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270835"
     CDS             1..475
                     /gene="LOC144109669"
                     /coded_by="XM_077642516.1:147..1574"
                     /db_xref="GeneID:144109669"
ORIGIN      
        1 mngfektenv eegaldnars stpnsnvagr epngnlspvs edkvlseppa plppprtsss
       61 hsylnkvckm aarteaplgk shwprpaphs ssfdectsme yqqvsavrkp arpkseills
      121 qekgsktprc psmtfegnmt fgrieayvrl eqlgegsyat vyrgysnlmg kvvalkeirl
      181 qpeegtpfta ireasllrgl khanivtlhd iihtkdtltf vfeyvhtdls qylekhpggl
      241 npknvklflf qllrglsych erlilhrdlk pqnlliseqg elkladfgla raksvpsrty
      301 shevvtlwyr ppdvllgstd ystsldmwgv gcifiemitg aaafpgvkdt tdqldkiwri
      361 lgtptedtwe gvsryknykl ykfgmypgqp lkqafpklae tlqadeianr flqlqpqnri
      421 saadalrhsy fadmppkvyd lpdqasiftv pgcklspety nlpmsvikaa aklrc
//
LOCUS       XP_077498648             443 aa            linear   INV 25-NOV-2025
DEFINITION  cyclin-dependent kinase 14-like isoform X5 [Amblyomma americanum].
ACCESSION   XP_077498648
VERSION     XP_077498648.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642522.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="cyclin-dependent kinase 14-like isoform X5"
                     /calculated_mol_wt=49437
     Region          207..>420
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(214..217,220,222,235,237,267,283..286,329,333..334,
                     336,346..347)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..443
                     /gene="LOC144109669"
                     /coded_by="XM_077642522.1:131..1462"
                     /db_xref="GeneID:144109669"
ORIGIN      
        1 mslsvssknw kpstkedkdr rlsriqrlrr rlscsfgrls lskevilpdg enenvavtrv
       61 tmngfekten veegaldnar sstpnsnvag repngnlspv sedkvlsepp aplppprtss
      121 shsylnkvck maarteaplg kshwprpaph sssfdectsm eyqqvsavrk parpkseill
      181 sqekgsktpr cpsmtfegnm tfgrieayvr leqlgegsya tvyrgysnlm gkvvalkeir
      241 lqpeegtpft aireasllrg lkhanivtlh diihtkdtlt fvfeyvhtdl sqylekhpgg
      301 lnpknvklfl fqllrglsyc herlilhrdl kpqnlliseq gelkladfgl araksvpsrt
      361 yshevvtlwy rppdvllgst dystsldmwg vgcifiemit gaaafpgvkd ttdqldkiwr
      421 pqnpvahdtd admdsgssrr rks
//
LOCUS       XP_077498662             677 aa            linear   INV 25-NOV-2025
DEFINITION  armadillo repeat-containing protein 8-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498662
VERSION     XP_077498662.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642536.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..677
                     /product="armadillo repeat-containing protein 8-like
                     isoform X1"
                     /calculated_mol_wt=75329
     Region          <50..>106
                     /region_name="SRP1"
                     /note="Karyopherin (importin) alpha [Intracellular
                     trafficking and secretion]; COG5064"
                     /db_xref="CDD:227396"
     Region          50..82
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Site            order(73,77,81,115,119,123,199,203)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293788"
     Region          90..155
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          174..205
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          <263..673
                     /region_name="COG5369"
                     /note="Uncharacterized conserved protein [Function
                     unknown]"
                     /db_xref="CDD:227663"
     Region          428..456
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          468..502
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(534,538,542,579,583,587,621,625,629)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293788"
     Region          550..590
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          596..630
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     CDS             1..677
                     /gene="LOC144109739"
                     /coded_by="XM_077642536.1:250..2283"
                     /db_xref="GeneID:144109739"
ORIGIN      
        1 mvtihqsymv vecnrsyidl lffhdpekcl egiiqlknsv igsnnqkatv irmglvprll
       61 qilreeshpq llcetaviln slakgteenv kalleagvlp vllqglvhqd qryveaclcc
      121 lrtlflsela pvecmyeepw avrhlllmat qmhatnqeci atilmavckt pdhqnylcah
      181 gavptltall cspvykvqlp tlrclthmcy qnpkvsqala tssyggrtlp dilvtlmard
      241 kptemqlaaa kcmtfisrag amhsedpriv fkalptlvrm ckkentpeer algaetlayl
      301 aevdtelqri asisdhlipt laelfkyqpp savvlykgpv pavtmkkleq evkvmqdlkq
      361 gafkayaalg andedirkki ietdslmenv vtnlsdtnpr vrlaavrclh slsrsvqqlr
      421 ttfqdhavwr plmrllhnap ddillvasst lcnlllefsp skerclqpil ecgavellcg
      481 ltrrdepalr lngcwalmnm afqadqkiks qilstlgtdq vfrllsdpdv svlmktlgll
      541 rnllsikqhi dhimdshgtq imqavilile gdhsadvkeq alcilaniad gdtakafima
      601 nedvlkkltt yiahsnvklq iaatfcisnl vwseeegare rqsklrefgv pkllqsllit
      661 sdttlfdkvk talqqft
//
LOCUS       XP_077498670             673 aa            linear   INV 25-NOV-2025
DEFINITION  armadillo repeat-containing protein 8-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077498670
VERSION     XP_077498670.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642544.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..673
                     /product="armadillo repeat-containing protein 8-like
                     isoform X2"
                     /calculated_mol_wt=74828
     Region          <50..>106
                     /region_name="SRP1"
                     /note="Karyopherin (importin) alpha [Intracellular
                     trafficking and secretion]; COG5064"
                     /db_xref="CDD:227396"
     Region          50..82
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Site            order(73,77,81,115,119,123,199,203)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293788"
     Region          90..155
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          174..205
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          <263..669
                     /region_name="COG5369"
                     /note="Uncharacterized conserved protein [Function
                     unknown]"
                     /db_xref="CDD:227663"
     Site            order(445,449,453,487,491,495,530,534,538)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293788"
     Region          462..499
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          505..539
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          546..586
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          592..626
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     CDS             1..673
                     /gene="LOC144109739"
                     /coded_by="XM_077642544.1:249..2270"
                     /db_xref="GeneID:144109739"
ORIGIN      
        1 mvtihqsymv vecnrsyidl lffhdpekcl egiiqlknsv igsnnqkatv irmglvprll
       61 qilreeshpq llcetaviln slakgteenv kalleagvlp vllqglvhqd qryveaclcc
      121 lrtlflsela pvecmyeepw avrhlllmat qmhatnqeci atilmavckt pdhqnylcah
      181 gavptltall cspvykvqlp tlrclthmcy qnpkvsqala tssyggrtlp dilvtlmard
      241 kptemqlaaa kcmtfisrag amhsedpriv fkalptlvrm ckkentpeer algaetlayl
      301 aevdtelqri asisdhlipt laelfkyqpp savvlykgpv pavtmkkleq evkvmqdlkq
      361 gafkayaalg andedirkki ietdslmenv vtnlsdtnpr vrlaavrclh slsrsvqqlr
      421 ttfqdhavwr plmrllhnap ddillvasst lcnlllefsp skepilecga vellcgltrr
      481 depalrlngc walmnmafqa dqkiksqils tlgtdqvfrl lsdpdvsvlm ktlgllrnll
      541 sikqhidhim dshgtqimqa vililegdhs advkeqalci laniadgdta kafimanedv
      601 lkklttyiah snvklqiaat fcisnlvwse eegarerqsk lrefgvpkll qsllitsdtt
      661 lfdkvktalq qft
//
LOCUS       XP_077498676             592 aa            linear   INV 25-NOV-2025
DEFINITION  armadillo repeat-containing protein 8-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077498676
VERSION     XP_077498676.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642550.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..592
                     /product="armadillo repeat-containing protein 8-like
                     isoform X3"
                     /calculated_mol_wt=65860
     Region          <50..>106
                     /region_name="SRP1"
                     /note="Karyopherin (importin) alpha [Intracellular
                     trafficking and secretion]; COG5064"
                     /db_xref="CDD:227396"
     Region          50..82
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Site            order(73,77,81,115,119,123,199,203)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293788"
     Region          90..155
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          174..205
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          <263..>463
                     /region_name="COG5369"
                     /note="Uncharacterized conserved protein [Function
                     unknown]"
                     /db_xref="CDD:227663"
     Region          386..414
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(445,449,453,494,498,502,536,540,544)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293788"
     Region          462..505
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          511..545
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     CDS             1..592
                     /gene="LOC144109739"
                     /coded_by="XM_077642550.1:250..2028"
                     /db_xref="GeneID:144109739"
ORIGIN      
        1 mvtihqsymv vecnrsyidl lffhdpekcl egiiqlknsv igsnnqkatv irmglvprll
       61 qilreeshpq llcetaviln slakgteenv kalleagvlp vllqglvhqd qryveaclcc
      121 lrtlflsela pvecmyeepw avrhlllmat qmhatnqeci atilmavckt pdhqnylcah
      181 gavptltall cspvykvqlp tlrclthmcy qnpkvsqala tssyggrtlp dilvtlmard
      241 kptemqlaaa kcmtfisrag amhsedpriv fkalptlvrm ckkentpeer algaetlayl
      301 aevdtelqri asisdhlipt laelfkyqpp savvlykgpv pavtmkkleq evkvmqdlkq
      361 gafkayaalg andedirkki ietdslmenv vtnlsdtnpr vrlaavrclh slsrsvqqlr
      421 ttfqdhavwr plmrllhnap ddillvasst lcnlllefsp skehidhimd shgtqimqav
      481 ililegdhsa dvkeqalcil aniadgdtak afimanedvl kklttyiahs nvklqiaatf
      541 cisnlvwsee egarerqskl refgvpkllq sllitsdttl fdkvktalqq ft
//
LOCUS       XP_077498681             567 aa            linear   INV 25-NOV-2025
DEFINITION  armadillo repeat-containing protein 8-like isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077498681
VERSION     XP_077498681.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642555.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..567
                     /product="armadillo repeat-containing protein 8-like
                     isoform X4"
                     /calculated_mol_wt=63192
     Region          <50..>106
                     /region_name="SRP1"
                     /note="Karyopherin (importin) alpha [Intracellular
                     trafficking and secretion]; COG5064"
                     /db_xref="CDD:227396"
     Region          50..82
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Site            order(73,77,81,115,119,123,199,203)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293788"
     Region          90..155
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          174..205
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          <263..>558
                     /region_name="COG5369"
                     /note="Uncharacterized conserved protein [Function
                     unknown]"
                     /db_xref="CDD:227663"
     Site            order(445,449,453,491,495,499,534,538,542)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293788"
     Region          462..503
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          509..543
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     CDS             1..567
                     /gene="LOC144109739"
                     /coded_by="XM_077642555.1:252..1955"
                     /db_xref="GeneID:144109739"
ORIGIN      
        1 mvtihqsymv vecnrsyidl lffhdpekcl egiiqlknsv igsnnqkatv irmglvprll
       61 qilreeshpq llcetaviln slakgteenv kalleagvlp vllqglvhqd qryveaclcc
      121 lrtlflsela pvecmyeepw avrhlllmat qmhatnqeci atilmavckt pdhqnylcah
      181 gavptltall cspvykvqlp tlrclthmcy qnpkvsqala tssyggrtlp dilvtlmard
      241 kptemqlaaa kcmtfisrag amhsedpriv fkalptlvrm ckkentpeer algaetlayl
      301 aevdtelqri asisdhlipt laelfkyqpp savvlykgpv pavtmkkleq evkvmqdlkq
      361 gafkayaalg andedirkki ietdslmenv vtnlsdtnpr vrlaavrclh slsrsvqqlr
      421 ttfqdhavwr plmrllhnap ddillvasst lcnlllefsp skerclqpil ecgavellcg
      481 ltrrdepalr lngcwalmnm afqadqkiks qilstlgtdq vfrllsdpdv svlmktlgll
      541 rnllsikqal qepemvlfvf nfcsank
//
LOCUS       XP_077498687             563 aa            linear   INV 25-NOV-2025
DEFINITION  armadillo repeat-containing protein 8-like isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077498687
VERSION     XP_077498687.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642561.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..563
                     /product="armadillo repeat-containing protein 8-like
                     isoform X5"
                     /calculated_mol_wt=62691
     Region          <50..>106
                     /region_name="SRP1"
                     /note="Karyopherin (importin) alpha [Intracellular
                     trafficking and secretion]; COG5064"
                     /db_xref="CDD:227396"
     Region          50..82
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Site            order(73,77,81,115,119,123,199,203)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293788"
     Region          90..155
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          174..205
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          <263..>554
                     /region_name="COG5369"
                     /note="Uncharacterized conserved protein [Function
                     unknown]"
                     /db_xref="CDD:227663"
     Site            order(445,449,453,487,491,495,530,534,538)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293788"
     Region          462..499
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          505..539
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     CDS             1..563
                     /gene="LOC144109739"
                     /coded_by="XM_077642561.1:250..1941"
                     /db_xref="GeneID:144109739"
ORIGIN      
        1 mvtihqsymv vecnrsyidl lffhdpekcl egiiqlknsv igsnnqkatv irmglvprll
       61 qilreeshpq llcetaviln slakgteenv kalleagvlp vllqglvhqd qryveaclcc
      121 lrtlflsela pvecmyeepw avrhlllmat qmhatnqeci atilmavckt pdhqnylcah
      181 gavptltall cspvykvqlp tlrclthmcy qnpkvsqala tssyggrtlp dilvtlmard
      241 kptemqlaaa kcmtfisrag amhsedpriv fkalptlvrm ckkentpeer algaetlayl
      301 aevdtelqri asisdhlipt laelfkyqpp savvlykgpv pavtmkkleq evkvmqdlkq
      361 gafkayaalg andedirkki ietdslmenv vtnlsdtnpr vrlaavrclh slsrsvqqlr
      421 ttfqdhavwr plmrllhnap ddillvasst lcnlllefsp skepilecga vellcgltrr
      481 depalrlngc walmnmafqa dqkiksqils tlgtdqvfrl lsdpdvsvlm ktlgllrnll
      541 sikqalqepe mvlfvfnfcs ank
//
LOCUS       XP_077498694             557 aa            linear   INV 25-NOV-2025
DEFINITION  armadillo repeat-containing protein 8-like isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077498694
VERSION     XP_077498694.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642568.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..557
                     /product="armadillo repeat-containing protein 8-like
                     isoform X6"
                     /calculated_mol_wt=61744
     Region          <50..>106
                     /region_name="SRP1"
                     /note="Karyopherin (importin) alpha [Intracellular
                     trafficking and secretion]; COG5064"
                     /db_xref="CDD:227396"
     Region          50..82
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Site            order(73,77,81,115,119,123,199,203)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293788"
     Region          90..155
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          174..205
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          231..261
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          273..302
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          <340..553
                     /region_name="COG5369"
                     /note="Uncharacterized conserved protein [Function
                     unknown]"
                     /db_xref="CDD:227663"
     Region          346..383
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Site            order(414,418,422,459,463,467,501,505,509)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293788"
     Region          430..470
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          476..510
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     CDS             1..557
                     /gene="LOC144109739"
                     /coded_by="XM_077642568.1:250..1923"
                     /db_xref="GeneID:144109739"
ORIGIN      
        1 mvtihqsymv vecnrsyidl lffhdpekcl egiiqlknsv igsnnqkatv irmglvprll
       61 qilreeshpq llcetaviln slakgteenv kalleagvlp vllqglvhqd qryveaclcc
      121 lrtlflsela pvecmyeepw avrhlllmat qmhatnqeci atilmavckt pdhqnylcah
      181 gavptltall cspvykvqlp tlrclthmcy qnpkvsqala tssyggrtlp dilvtlmard
      241 kptemqlaaa kcmtfisrag amhsedpriv fkalptlvrm ckkentpeer algaetlayl
      301 aevdtelqri asisdhlipt laelfkyqpp savvlykgpv pavtmkkpil ecgavellcg
      361 ltrrdepalr lngcwalmnm afqadqkiks qilstlgtdq vfrllsdpdv svlmktlgll
      421 rnllsikqhi dhimdshgtq imqavilile gdhsadvkeq alcilaniad gdtakafima
      481 nedvlkkltt yiahsnvklq iaatfcisnl vwseeegare rqsklrefgv pkllqsllit
      541 sdttlfdkvk talqqft
//
LOCUS       XP_077498698              77 aa            linear   INV 25-NOV-2025
DEFINITION  pre-mRNA-splicing factor CWC25 homolog [Amblyomma americanum].
ACCESSION   XP_077498698
VERSION     XP_077498698.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642572.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..77
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..77
                     /product="pre-mRNA-splicing factor CWC25 homolog"
                     /calculated_mol_wt=8855
     CDS             1..77
                     /gene="LOC144109777"
                     /coded_by="XM_077642572.1:112..345"
                     /db_xref="GeneID:144109777"
ORIGIN      
        1 mmenahwrde qcktfvnhyc kseaveeekq ykrtgegfir pmlaeaadsg svekrikqkl
       61 ytvqrssgam dshfark
//
LOCUS       XP_077498706             228 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109787 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498706
VERSION     XP_077498706.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642580.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..228
                     /product="uncharacterized protein LOC144109787 isoform X1"
                     /calculated_mol_wt=24660
     Region          4..80
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..228
                     /gene="LOC144109787"
                     /coded_by="XM_077642580.1:153..839"
                     /db_xref="GeneID:144109787"
ORIGIN      
        1 mpsccvpgcr srtpgqepgv tfhtfpkdpe qrrqwteavr pgqrdwepak wtcvcskhfl
       61 pehfdrmspl vvriradavp lqqeplsqqp gdgtavaata qhgaqqaltq shtfpasplq
      121 qapmdvaesq etlagpsqqa pmdvtesqet lpgpsqqasm dvatmldiqp gpsqafqltp
      181 vsfisscvvs iqvyslhevr crcnsamysr tcsclsgpvl tgdssrvq
//
LOCUS       XP_077498711             212 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109787 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077498711
VERSION     XP_077498711.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642585.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..212
                     /product="uncharacterized protein LOC144109787 isoform X2"
                     /calculated_mol_wt=22887
     Region          4..80
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..212
                     /gene="LOC144109787"
                     /coded_by="XM_077642585.1:153..791"
                     /db_xref="GeneID:144109787"
ORIGIN      
        1 mpsccvpgcr srtpgqepgv tfhtfpkdpe qrrqwteavr pgqrdwepak wtcvcskhfl
       61 pehfdrmspl vvriradavp lqqeplsqqp gdgtavaata qhgaqqaltq shtfpasplq
      121 qapmdvaesq etlagpsqqa pmdvtesqet lpgpsqqasm dvatmldiqp gpsqafqltp
      181 elptlptasa svgtprrrrp amvngpvkca yf
//
LOCUS       XP_077498723             483 aa            linear   INV 25-NOV-2025
DEFINITION  actin binding protein 1 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077498723
VERSION     XP_077498723.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642597.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (DAPXPI010009286.1) annotated
            using gene prediction method: Gnomon, supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 26 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..483
                     /product="actin binding protein 1 isoform X1"
                     /calculated_mol_wt=53968
     Region          20..155
                     /region_name="ADF_drebrin_like"
                     /note="ADF homology domain of drebrin and actin-binding
                     protein 1 (abp1); cd11281"
                     /db_xref="CDD:200437"
     Site            order(95,109,136,139)
                     /site_type="other"
                     /note="putative F-actin interface [polypeptide binding]"
                     /db_xref="CDD:200437"
     Region          <245..429
                     /region_name="PTZ00436"
                     /note="60S ribosomal protein L19-like protein;
                     Provisional"
                     /db_xref="CDD:185616"
     Region          428..481
                     /region_name="SH3_Abp1_eu"
                     /note="Src homology 3 domain of eumetazoan Actin-binding
                     protein 1; cd11960"
                     /db_xref="CDD:212893"
     Site            order(433,435,438,441..442,460..461,473,475,477..478)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212893"
     CDS             1..483
                     /gene="Abp1"
                     /coded_by="XM_077642597.1:49..1500"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 26 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144109809"
ORIGIN      
        1 mffrrhclqt pssmlanmav nlakhrdell sawkevvddk agtnwalfgy dkqtydlcvv
       61 gkgaggleel teelncgkvm yafckvqdps tslskfvlin wqgegvplvk kgccanhlmd
      121 isnffrgahi tvtarneddv epslivekls kctvssfnlr ersdptesar pigsvykriq
      181 pareiaster ekfwmkeqee ekkrikeekl kaeaaksrla eqvkerelkd aqareewfke
      241 rslsidkmre aeknaqnsth nkvnkklweq qlqedkhdee erkcmsqslr nrrseeaqal
      301 iaqrttdaka mfepstctga yasqasaals eqtnlkgtas qaeapavqnn hgaalasspt
      361 kltytrnqlq daqttclgaa ppepahnqsw ededgiaevs rpaseavegp glnqaqpasv
      421 etsepeirar alydyqaadd teisfdpddi ithieqideg wwqglgpdgt yglfpanyve
      481 lie
//
LOCUS       XP_077498727             437 aa            linear   INV 25-NOV-2025
DEFINITION  actin binding protein 1 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077498727
VERSION     XP_077498727.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642601.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (DAPXPI010009286.1) annotated
            using gene prediction method: Gnomon, supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 26 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..437
                     /product="actin binding protein 1 isoform X2"
                     /calculated_mol_wt=48917
     Region          20..>127
                     /region_name="ADF_drebrin_like"
                     /note="ADF homology domain of drebrin and actin-binding
                     protein 1 (abp1); cd11281"
                     /db_xref="CDD:200437"
     Region          <199..383
                     /region_name="PTZ00436"
                     /note="60S ribosomal protein L19-like protein;
                     Provisional"
                     /db_xref="CDD:185616"
     Region          382..435
                     /region_name="SH3_Abp1_eu"
                     /note="Src homology 3 domain of eumetazoan Actin-binding
                     protein 1; cd11960"
                     /db_xref="CDD:212893"
     Site            order(387,389,392,395..396,414..415,427,429,431..432)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212893"
     CDS             1..437
                     /gene="Abp1"
                     /coded_by="XM_077642601.1:50..1363"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 26 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144109809"
ORIGIN      
        1 mffrrhclqt pssmlanmav nlakhrdell sawkevvddk agtnwalfgy dkqtydlcvv
       61 gkgaggleel teelncgkvm yafckvqdps tslskfvlin wqgegvplvk kgccanhlmd
      121 isnffrgsvy kriqpareia sterekfwmk eqeeekkrik eeklkaeaak srlaeqvker
      181 elkdaqaree wfkerslsid kmreaeknaq nsthnkvnkk lweqqlqedk hdeeerkcms
      241 qslrnrrsee aqaliaqrtt dakamfepst ctgayasqas aalseqtnlk gtasqaeapa
      301 vqnnhgaala ssptkltytr nqlqdaqttc lgaappepah nqswededgi aevsrpasea
      361 vegpglnqaq pasvetsepe iraralydyq aaddteisfd pddiithieq idegwwqglg
      421 pdgtyglfpa nyvelie
//
LOCUS       XP_077498733             634 aa            linear   INV 25-NOV-2025
DEFINITION  Hermansky-Pudlak syndrome 1 protein isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498733
VERSION     XP_077498733.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642607.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..634
                     /product="Hermansky-Pudlak syndrome 1 protein isoform X1"
                     /calculated_mol_wt=71474
     Region          2..136
                     /region_name="Fuz_longin_1"
                     /note="First Longin domain of FUZ, MON1 and HPS1;
                     pfam19036"
                     /db_xref="CDD:465953"
     CDS             1..634
                     /gene="HPS1"
                     /coded_by="XM_077642607.1:100..2004"
                     /db_xref="GeneID:144109821"
ORIGIN      
        1 mkgflmfdtl ndvvykqmde dllkhlteas arnglipega seqaahpser itndvlvqlf
       61 splvathrvm kermrnaysc iyckdqtlcv ftefydwtvl gissekedse avcqkrlkvf
      121 valiqrlfgp alqelkphta sgsprsqlla evmetycrlr qedqaflfea leqvvvnrdi
      181 sssctkllqe ilemvqrccl kgetachsfl lfnyrlvssf srrnstrlsh gdmlllflil
      241 rsfhgvrhat gddrqekngr ihsllvffat alhssiphvv hsivitdail vvvvsevsnk
      301 vlladslssf lkqlhsiqkk pedgrcrltv dilenaitrl sdtfrraean srpyhpmvag
      361 snlsenlhil hdarlhksee icvslitrkw ahlkmsgfve ylsgsnnael aarseealll
      421 lkknvqtafk elvlnpelar eaeprpmlas slelvqavtq eklenymsfl lvkaaeglnv
      481 ssfsaqypgl lhfvyinrta nrmlatsina aelhamvwrg wesaqryshe gsftamwadg
      541 dlhfsytlwf enhagktvkp kcavnpaslq qmaypgvvss sfygqlmryc fpdvsleqls
      601 clefmcahan sipastileq lqqlplslrd lcfq
//
LOCUS       XP_077498736             608 aa            linear   INV 25-NOV-2025
DEFINITION  Hermansky-Pudlak syndrome 1 protein isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077498736
VERSION     XP_077498736.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642610.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..608
                     /product="Hermansky-Pudlak syndrome 1 protein isoform X2"
                     /calculated_mol_wt=68591
     Region          2..136
                     /region_name="Fuz_longin_1"
                     /note="First Longin domain of FUZ, MON1 and HPS1;
                     pfam19036"
                     /db_xref="CDD:465953"
     CDS             1..608
                     /gene="HPS1"
                     /coded_by="XM_077642610.1:100..1926"
                     /db_xref="GeneID:144109821"
ORIGIN      
        1 mkgflmfdtl ndvvykqmde dllkhlteas arnglipega seqaahpser itndvlvqlf
       61 splvathrvm kermrnaysc iyckdqtlcv ftefydwtvl gissekedse avcqkrlkvf
      121 valiqrlfgp alqelkphta sgsprsqlla evmetycrlr qedqaflfea leqvvvnrdi
      181 sssctkllqe ilemvqrccl kgetachsfl lfnyrlvssf srrnstrlsh gdmlllflil
      241 rsfhgvrhat gddrqekngr ihsllvffat alhssiphvv hsivitdail vvvvsevsnk
      301 vlladslssf lkqlhsiqkk pedgrcrltv dilenaitrl sdtfrraelh kseeicvsli
      361 trkwahlkms gfveylsgsn naelaarsee allllkknvq tafkelvlnp elareaeprp
      421 mlasslelvq avtqekleny msfllvkaae glnvssfsaq ypgllhfvyi nrtanrmlat
      481 sinaaelham vwrgwesaqr yshegsftam wadgdlhfsy tlwfenhagk tvkpkcavnp
      541 aslqqmaypg vvsssfygql mrycfpdvsl eqlsclefmc ahansipast ileqlqqlpl
      601 slrdlcfq
//
LOCUS       XP_077498743             134 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109835 [Amblyomma americanum].
ACCESSION   XP_077498743
VERSION     XP_077498743.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642617.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..134
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..134
                     /product="uncharacterized protein LOC144109835"
                     /calculated_mol_wt=14328
     CDS             1..134
                     /gene="LOC144109835"
                     /coded_by="XM_077642617.1:1490..1894"
                     /db_xref="GeneID:144109835"
ORIGIN      
        1 mfslesnnmw lvnsqhaaeg gleglpttph lavigssmgd ikkiivavds trslekamgl
       61 llgaffvfnv ayppdcpltm ellqrylgqs nptkgrgkgr acgiapklls llnalqfmnk
      121 casrnvnlfl lhgl
//
LOCUS       XP_077498749             134 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109835 [Amblyomma americanum].
ACCESSION   XP_077498749
VERSION     XP_077498749.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642623.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..134
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..134
                     /product="uncharacterized protein LOC144109835"
                     /calculated_mol_wt=14328
     CDS             1..134
                     /gene="LOC144109835"
                     /coded_by="XM_077642623.1:986..1390"
                     /db_xref="GeneID:144109835"
ORIGIN      
        1 mfslesnnmw lvnsqhaaeg gleglpttph lavigssmgd ikkiivavds trslekamgl
       61 llgaffvfnv ayppdcpltm ellqrylgqs nptkgrgkgr acgiapklls llnalqfmnk
      121 casrnvnlfl lhgl
//
LOCUS       XP_077498758             124 aa            linear   INV 25-NOV-2025
DEFINITION  BEN domain-containing protein 5-like [Amblyomma americanum].
ACCESSION   XP_077498758
VERSION     XP_077498758.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642632.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..124
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..124
                     /product="BEN domain-containing protein 5-like"
                     /calculated_mol_wt=14159
     CDS             1..124
                     /gene="LOC144109850"
                     /coded_by="XM_077642632.1:208..582"
                     /db_xref="GeneID:144109850"
ORIGIN      
        1 mfavvrfahd idkklyvipa kditnfnpkn ntdfnkrsvy tafwrdpldg kdsshystqv
       61 lmlgesekei qermkkkrla mpkildshds ssetceeeta aqkkkffrsl eakansagiq
      121 ksrv
//
LOCUS       XP_077498761             111 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109859 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498761
VERSION     XP_077498761.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642635.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..111
                     /product="uncharacterized protein LOC144109859 isoform X1"
                     /calculated_mol_wt=12379
     CDS             1..111
                     /gene="LOC144109859"
                     /coded_by="XM_077642635.1:1050..1385"
                     /db_xref="GeneID:144109859"
ORIGIN      
        1 mrflgcsvtt micklepflq atqdkhirgt lfdpsltlrk rkkdvsgklp vespthatlv
       61 sdretrltmd kmtkgsgqkr ipedevaadl rillahshrt qramcddssd l
//
LOCUS       XP_077498765             111 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109859 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498765
VERSION     XP_077498765.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642639.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..111
                     /product="uncharacterized protein LOC144109859 isoform X1"
                     /calculated_mol_wt=12379
     CDS             1..111
                     /gene="LOC144109859"
                     /coded_by="XM_077642639.1:1037..1372"
                     /db_xref="GeneID:144109859"
ORIGIN      
        1 mrflgcsvtt micklepflq atqdkhirgt lfdpsltlrk rkkdvsgklp vespthatlv
       61 sdretrltmd kmtkgsgqkr ipedevaadl rillahshrt qramcddssd l
//
LOCUS       XP_077498767             111 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109859 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498767
VERSION     XP_077498767.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642641.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..111
                     /product="uncharacterized protein LOC144109859 isoform X1"
                     /calculated_mol_wt=12379
     CDS             1..111
                     /gene="LOC144109859"
                     /coded_by="XM_077642641.1:940..1275"
                     /db_xref="GeneID:144109859"
ORIGIN      
        1 mrflgcsvtt micklepflq atqdkhirgt lfdpsltlrk rkkdvsgklp vespthatlv
       61 sdretrltmd kmtkgsgqkr ipedevaadl rillahshrt qramcddssd l
//
LOCUS       XP_077498772             111 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109859 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498772
VERSION     XP_077498772.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642646.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..111
                     /product="uncharacterized protein LOC144109859 isoform X1"
                     /calculated_mol_wt=12379
     CDS             1..111
                     /gene="LOC144109859"
                     /coded_by="XM_077642646.1:166..501"
                     /db_xref="GeneID:144109859"
ORIGIN      
        1 mrflgcsvtt micklepflq atqdkhirgt lfdpsltlrk rkkdvsgklp vespthatlv
       61 sdretrltmd kmtkgsgqkr ipedevaadl rillahshrt qramcddssd l
//
LOCUS       XP_077498775              97 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109859 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077498775
VERSION     XP_077498775.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642649.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..97
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..97
                     /product="uncharacterized protein LOC144109859 isoform X2"
                     /calculated_mol_wt=10840
     CDS             1..97
                     /gene="LOC144109859"
                     /coded_by="XM_077642649.1:1087..1380"
                     /db_xref="GeneID:144109859"
ORIGIN      
        1 mymwallamq nlsrgtlfdp sltlrkrkkd vsgklpvesp thatlvsdre trltmdkmtk
       61 gsgqkriped evaadlrill ahshrtqram cddssdl
//
LOCUS       XP_077498790             230 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109897 [Amblyomma americanum].
ACCESSION   XP_077498790
VERSION     XP_077498790.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642664.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..230
                     /product="uncharacterized protein LOC144109897"
                     /calculated_mol_wt=27131
     Region          <71..>152
                     /region_name="DUF5401"
                     /note="Family of unknown function (DUF5401); pfam17380"
                     /db_xref="CDD:375164"
     CDS             1..230
                     /gene="LOC144109897"
                     /coded_by="XM_077642664.1:134..826"
                     /db_xref="GeneID:144109897"
ORIGIN      
        1 mppnakdvnn rnakrrlqra tetdeereer lakrraqyaa rrqryegvse astsdstsgi
       61 gdsvsistss erwnatkrqr rametieerq qrlekerlrq egrnvanrqk raletpeere
      121 qrlekerprr etekenrkrr rieeaamrql qqrqtseeia eerrrehatr vaefdaatle
      181 fakrftnnpf gyacsvcarl whkekltpvt pamfptlcqa fpewtdnqla
//
LOCUS       XP_077498801             194 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109904 [Amblyomma americanum].
ACCESSION   XP_077498801
VERSION     XP_077498801.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642675.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..194
                     /product="uncharacterized protein LOC144109904"
                     /calculated_mol_wt=21661
     Region          13..102
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..194
                     /gene="LOC144109904"
                     /coded_by="XM_077642675.1:126..710"
                     /db_xref="GeneID:144109904"
ORIGIN      
        1 mpysartssg qhcvvvgcgn nqrkrkniad stcakhavsr sqcgcdmfml hrfpvdpeln
       61 rqwtslvnrk dfapttnsrv cslhfvdgkr teqnplpmln lgyerkvihg rrrlmrddrg
      121 pppvkrkrqe spsdkseasd avqqtpphnv vcighhsvsl pfshglsvsm halivplllk
      181 lncshlllsf tgyq
//
LOCUS       XP_077498829             171 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109931 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498829
VERSION     XP_077498829.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642703.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..171
                     /product="uncharacterized protein LOC144109931 isoform X1"
                     /calculated_mol_wt=19038
     CDS             1..171
                     /gene="LOC144109931"
                     /coded_by="XM_077642703.1:54..569"
                     /db_xref="GeneID:144109931"
ORIGIN      
        1 mlshtwmvhf qsvlhdtath sayfdrsaqe aslskpkrsv fmcdlqaqrd mavsvllhvi
       61 valgtllaia rdvvqvytca hdcmlrdfap hrfhfsgnns ppsmdmqgpq psemlytrha
      121 ravtfnrnla ciadlrpsfa flvccvhldg dtkgtcrstv vfreppglln f
//
LOCUS       XP_077498837              74 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109931 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077498837
VERSION     XP_077498837.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642711.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..74
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..74
                     /product="uncharacterized protein LOC144109931 isoform X2"
                     /calculated_mol_wt=8336
     CDS             1..74
                     /gene="LOC144109931"
                     /coded_by="XM_077642711.1:295..519"
                     /db_xref="GeneID:144109931"
ORIGIN      
        1 mtacfgtsrr taftfleita rrvckcrcre dlvqqilhfr dcqcwfrgaa dcimaaddgl
       61 gecsfnrrgt qhal
//
LOCUS       XP_077498849             124 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109942 [Amblyomma americanum].
ACCESSION   XP_077498849
VERSION     XP_077498849.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642723.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..124
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..124
                     /product="uncharacterized protein LOC144109942"
                     /calculated_mol_wt=13623
     CDS             1..124
                     /gene="LOC144109942"
                     /coded_by="XM_077642723.1:161..535"
                     /db_xref="GeneID:144109942"
ORIGIN      
        1 mpwsmqhglc hslcsvwrch ihqiaeavaa vqkwrplpvf asimpgrara gfcglclqrr
       61 aekkghlkeq hrvntcgpsr lpaeavaavq kwrplpvfap impgrahagf cglcsrdapr
      121 krat
//
LOCUS       XP_077498871             729 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109959 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498871
VERSION     XP_077498871.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642745.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..729
                     /product="uncharacterized protein LOC144109959 isoform X1"
                     /calculated_mol_wt=80297
     Region          64..151
                     /region_name="retropepsin_like_LTR_1"
                     /note="Retropepsins_like_LTR; pepsin-like aspartate
                     protease from retrotransposons with long terminal repeats;
                     cd05481"
                     /db_xref="CDD:133148"
     Site            78..80
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133148"
     Site            78
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133148"
     Region          337..389
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          413..496
                     /region_name="rve"
                     /note="Integrase core domain; pfam00665"
                     /db_xref="CDD:459897"
     CDS             1..729
                     /gene="LOC144109959"
                     /coded_by="XM_077642745.1:140..2329"
                     /db_xref="GeneID:144109959"
ORIGIN      
        1 mqptaraeav sclrkrcntc rklhhfaicc paralvnevg aqfndefdvl dvcidscitg
       61 dwtveakvgg rkalfkvdtg aqasllpfsi yrklktaeln ptssvlrayn ggiitnfgtv
      121 qkitvgdaat tvkfyvmkng rrailglhac ealglvqrtv dtvntsaeye aikhfrhvfe
      181 glgclkqpys mvlqpdatpv vqparrvpls lrqplrdelq rmeragiitk edgptdwyvp
      241 gkhlvladml srsttpggvd nagatedvev halqllngmv tvttqqklak etardcylqt
      301 vvsnlsagnp iqgelkpfss elsvingilf kgtkaviprs mrqemltrih aghlglnkck
      361 erarllvfwq glnndiavll qncstcrkfa yqqakeplim rpvpqcawyr vgadifcfgg
      421 nsyvvvydal snfpdverlp dtsarsvvaa lsamfarygi pielctdngp qfssqefaaf
      481 akeydfahvt ssphypqsng laekgvqivk rimkktqdar hdfwlgllay rstplidgqs
      541 pgellqgrrl rsnlpdfggi ktievkkhrq tpkgqplpql gkgdvvrlrd ttwsqkgkvv
      601 geaaprschv vtesrrvlrr nrrhllhsse qyaetsdddt dddcadeadp narnspesvt
      661 pqpygttsaa pqqppglcdq hlahgatpsa sithpeqpts vislrrstrt akppqrlryd
      721 aafnqlslt
//
LOCUS       XP_077498882             607 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109959 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077498882
VERSION     XP_077498882.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642756.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..607
                     /product="uncharacterized protein LOC144109959 isoform X2"
                     /calculated_mol_wt=66549
     Region          64..151
                     /region_name="retropepsin_like_LTR_1"
                     /note="Retropepsins_like_LTR; pepsin-like aspartate
                     protease from retrotransposons with long terminal repeats;
                     cd05481"
                     /db_xref="CDD:133148"
     Site            78..80
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133148"
     Site            78
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133148"
     Region          <320..374
                     /region_name="rve"
                     /note="Integrase core domain; pfam00665"
                     /db_xref="CDD:459897"
     CDS             1..607
                     /gene="LOC144109959"
                     /coded_by="XM_077642756.1:140..1963"
                     /db_xref="GeneID:144109959"
ORIGIN      
        1 mqptaraeav sclrkrcntc rklhhfaicc paralvnevg aqfndefdvl dvcidscitg
       61 dwtveakvgg rkalfkvdtg aqasllpfsi yrklktaeln ptssvlrayn ggiitnfgtv
      121 qkitvgdaat tvkfyvmkng rrailglhac ealglvqrtv dtvntsaeye aikhfrhvfe
      181 glgclkqpys mvlqpdatpv vqparrvpls lrqplrdelq rmeragiitk edgptdwyvp
      241 gkhlvladml srsttpggvd nagatedvev halqllngmv tvttqqklak etardcylqt
      301 vvsnlsagnp iqdverlpdt sarsvvaals amfarygipi elctdngpqf ssqefaafak
      361 eydfahvtss phypqsngla ekgvqivkri mkktqdarhd fwlgllayrs tplidgqspg
      421 ellqgrrlrs nlpdfggikt ievkkhrqtp kgqplpqlgk gdvvrlrdtt wsqkgkvvge
      481 aaprschvvt esrrvlrrnr rhllhsseqy aetsdddtdd dcadeadpna rnspesvtpq
      541 pygttsaapq qppglcdqhl ahgatpsasi thpeqptsvi slrrstrtak ppqrlrydaa
      601 fnqlslt
//
LOCUS       XP_077498894             228 aa            linear   INV 25-NOV-2025
DEFINITION  mite allergen Lep d 7-like [Amblyomma americanum].
ACCESSION   XP_077498894
VERSION     XP_077498894.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642768.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..228
                     /product="mite allergen Lep d 7-like"
                     /calculated_mol_wt=25530
     Region          43..220
                     /region_name="Grp7_allergen"
                     /note="Group 7 allergen; pfam16984"
                     /db_xref="CDD:319052"
     CDS             1..228
                     /gene="LOC144109962"
                     /coded_by="XM_077642768.1:80..766"
                     /db_xref="GeneID:144109962"
ORIGIN      
        1 mhslvitaae igrrpampml pwllclvata lasqnqsvae qhanevfdqi lesvsqserl
       61 dplqlpeqva gfdrtflvrf hgetrlyntt ltglstvrrt gncrfqmddk gldvladvgv
      121 galdldskah vkfmgrgptv dlhvhidyvr vllealqvdg vvqlrqfkvq ellgfalkin
      181 glgplsymfn ffstyftmlf kgivrsrvek vlsaqiekki relkhslf
//
LOCUS       XP_077498905             479 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109973 [Amblyomma americanum].
ACCESSION   XP_077498905
VERSION     XP_077498905.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642779.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..479
                     /product="uncharacterized protein LOC144109973"
                     /calculated_mol_wt=54016
     CDS             1..479
                     /gene="LOC144109973"
                     /coded_by="XM_077642779.1:129..1568"
                     /db_xref="GeneID:144109973"
ORIGIN      
        1 mtskclvtfr ernfvlpfdg tltrrglver vgdhelfrgi dvatliftmf eedfnvfvdi
       61 pedfeirdra kikvsedcqv riadvldepq qaeqsrvtat ysltyslpav pndikfsier
      121 hqsgqyfrar srvvqwlyhd lclytmypgk lyneaaqalv srypnladss gtgydswrea
      181 lrfkakyerr kiriqqndga dstpskktmk dgkvatvggn alqrvsrpsv atapdgeded
      241 siaghiegmk savqkarpdm ayiidcmrrt fptrrkwigs edpsvevaie kfpalamssi
      301 aqlefelits vpvlerleea lqrtkekivh aarkkrhleg fledfdarmd gtseaavyet
      361 tvtaavcllp smvkervetf vrpfdpaavh yiptvvhkgg iltttdfsvc lekicikets
      421 llaavatqma lywafnivfd kkaqrsfdll crlinvdsgl rptplvrlaq tvlgkrvse
//
LOCUS       XP_077498918             654 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109989 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498918
VERSION     XP_077498918.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642792.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..654
                     /product="uncharacterized protein LOC144109989 isoform X1"
                     /calculated_mol_wt=73386
     Region          13..57
                     /region_name="ZnF_BED"
                     /note="BED zinc finger; smart00614"
                     /db_xref="CDD:214746"
     CDS             1..654
                     /gene="LOC144109989"
                     /coded_by="XM_077642792.1:739..2703"
                     /db_xref="GeneID:144109989"
ORIGIN      
        1 mahelrcail dgkyftvtss dedkvkakcs fcktaisgay sstsnfklhl krvhpvelqs
       61 fevykegwkk tttkrkadta nekmlqtklf cstrsklsvp evdnlivrfv vdglhslstv
      121 eepgfvalit glrpgstvis rrtlgrrtde ewekmlgdvr lklsdqdyva ttadiwstph
      181 rsfmgvtvhr idhnslsrrs lalacrrfag rhtydkigel ledirqdfdi shdkivatvt
      241 dnasnfvkaf kefglrseii dedgaaddls fesvtepdlg ledgeitlpr hvrcachtls
      301 lvagsdvkva lstvsferih ssamikcsal wncarrpkss eiivsalghs letpcatrwn
      361 slydsikdll rskgklrdlc ntlklqvfqe rdfafllqyc dvlqpiasal trlqaeadcf
      421 fgellptllq vesklhekaa adyvyckpil daarngftrr fdhllkmkps akeatvaavl
      481 hpyfklrwlp aemedfrrha qklteeavvd yvstaqaahs sareavgssa pgasnasada
      541 ffsfedtsgy tsrssqealv kleflnylqd prvdvamlhs yplirgafvk yntatcssap
      601 verlfsfasl iarphrgals drrfeqmllr psskrneyfl pnkniqpfyl lfsf
//
LOCUS       XP_077498927              57 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144109989 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077498927
VERSION     XP_077498927.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642801.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..57
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..57
                     /product="uncharacterized protein LOC144109989 isoform X2"
                     /calculated_mol_wt=6071
     CDS             1..57
                     /gene="LOC144109989"
                     /coded_by="XM_077642801.1:744..917"
                     /db_xref="GeneID:144109989"
ORIGIN      
        1 mahelrcail dgkyftvtss dedkvkakcs fcktaisgay sstsnfklhl kiqlags
//
LOCUS       XP_077498932             225 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144110003 [Amblyomma americanum].
ACCESSION   XP_077498932
VERSION     XP_077498932.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642806.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..225
                     /product="uncharacterized protein LOC144110003"
                     /calculated_mol_wt=25983
     CDS             1..225
                     /gene="LOC144110003"
                     /coded_by="XM_077642806.1:19..696"
                     /db_xref="GeneID:144110003"
ORIGIN      
        1 mdhilikwtq eemwdvypir amvntavgfr lltepgaidd vrgtielftw kdseppapae
       61 vldigkarvl etkrarlasa glrdegdvmt eslecspnee fcsqcenmrr eldvvkrrnk
      121 dleaahesqk vlkklkkmld dmksidaisa vrpcpqhken vrdeiiknyl kniqpdmstv
      181 teykkrdppl pstsrqfhcc dldttkrrrg egernlfhfr iqlsw
//
LOCUS       XP_077498951             189 aa            linear   INV 25-NOV-2025
DEFINITION  piwi-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077498951
VERSION     XP_077498951.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642825.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..189
                     /product="piwi-like protein 1"
                     /calculated_mol_wt=21631
     Region          <25..172
                     /region_name="Piwi-like"
                     /note="PIWI domain. Domain found in proteins involved in
                     RNA silencing. RNA silencing refers to a group of related
                     gene-silencing mechanisms mediated by short RNA molecules,
                     including siRNAs, miRNAs, and heterochromatin-related
                     guide RNAs. The central component...; cl00628"
                     /db_xref="CDD:412485"
     CDS             1..189
                     /gene="LOC144110018"
                     /coded_by="XM_077642825.1:135..704"
                     /db_xref="GeneID:144110018"
ORIGIN      
        1 mrlrrilrqw arrqrqderh qpvrifrtgd vvgdqsdrgl aarglpwlva slqglfpgvk
       61 hklafvlvek ristrfflps rehvtnpppg tvvdsevtrp erydfflvsq svrqgtaapt
      121 hynaiydttg lkpdhmqrla yklthlyfnw pgtirvpapc qyaqklafla gqslhaehna
      181 rlsstlfyl
//
LOCUS       XP_077498960             138 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144110029 [Amblyomma americanum].
ACCESSION   XP_077498960
VERSION     XP_077498960.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642834.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..138
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..138
                     /product="uncharacterized protein LOC144110029"
                     /calculated_mol_wt=14876
     CDS             1..138
                     /gene="LOC144110029"
                     /coded_by="XM_077642834.1:259..675"
                     /db_xref="GeneID:144110029"
ORIGIN      
        1 mlqasasraa krvrlccfva eccfvaltke trrcvieges tfnaglivcc gikkvtdtvv
       61 nveslclqts amkgpphcvn vavckdtgsv kvsasissst gsinsfldsh semmdafnpt
      121 krpkrqtrrp krekaerc
//
LOCUS       XP_077498993             685 aa            linear   INV 25-NOV-2025
DEFINITION  MICOS complex subunit MIC60-1-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077498993
VERSION     XP_077498993.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642867.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..685
                     /product="MICOS complex subunit MIC60-1-like isoform X1"
                     /calculated_mol_wt=77041
     Region          41..667
                     /region_name="Mitofilin"
                     /note="Mitochondrial inner membrane protein; pfam09731"
                     /db_xref="CDD:430783"
     CDS             1..685
                     /gene="LOC144110057"
                     /coded_by="XM_077642867.1:105..2162"
                     /db_xref="GeneID:144110057"
ORIGIN      
        1 mwrstrqvsw rcsrllpgnr hkarslvrlq stsssgrssa ktivitagva avgiggtlay
       61 akvdsdfrrk lessipygds llgsilgpaq gavrplpaev vkekalessl lrkrhpvepa
      121 rgdqrpalpp pppiavpppt pvkektpspq dleaeaqkql qdikernerl nfaleqslre
      181 aqaelekytk davvaeleak eaaqsyarkl yraideards edqymwdeal qakeaklaav
      241 qkaavkseqa wkclenlqsa ikkgqedket hsnlalspaq haakvaleql rnaesqsrsa
      301 keaartatdy kdaveraqlh fqkeieallp etkfseqagq klsedelnll lahahrkien
      361 ltkahakiqv teqeraehfq rqqervardl drqraeldam levklvkqqe afehelqqql
      421 rrqvaahteh lkealeeqke elsrtftlel ekkmveeraq hnaalsssva klqgmeeylr
      481 ardeldrlsq qakglwlacq glrhtlvhgp aggpqslstd vqlveraapm spyvplvlas
      541 ipeealargv ypevalkerf arveracqrv alvddkcpss llrylasylq slciiypkel
      601 pqeeltdeqr vnpemwdtfd vlsrvrywlq henleqalry asqlrgqprl vaqdwihelr
      661 lhlearqaas almafaaati aedae
//
LOCUS       XP_077498999             684 aa            linear   INV 25-NOV-2025
DEFINITION  MICOS complex subunit MIC60-1-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077498999
VERSION     XP_077498999.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642873.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..684
                     /product="MICOS complex subunit MIC60-1-like isoform X2"
                     /calculated_mol_wt=76970
     Region          41..666
                     /region_name="Mitofilin"
                     /note="Mitochondrial inner membrane protein; pfam09731"
                     /db_xref="CDD:430783"
     CDS             1..684
                     /gene="LOC144110057"
                     /coded_by="XM_077642873.1:105..2159"
                     /db_xref="GeneID:144110057"
ORIGIN      
        1 mwrstrqvsw rcsrllpgnr hkarslvrlq stsssgrssa ktivitagva avgiggtlay
       61 akvdsdfrrk lessipygds llgsilgpaq gavrplpaev vkekalessl lrkrhpvepa
      121 rgdqrpalpp pppiavpppt pvkektpspq dleaeaqkql qdikernerl nfaleqslre
      181 aqaelekytk davvaeleak eaaqsyarkl yraideards edqymwdeal qakeaklaav
      241 qkaavkseqa wkclenlqsa ikkgqedket hsnlalspaq haakvaleql rnaesqsrsa
      301 keaartatdy kdaveraqlh fqkeieallp etkfseqgqk lsedelnlll ahahrkienl
      361 tkahakiqvt eqeraehfqr qqervardld rqraeldaml evklvkqqea fehelqqqlr
      421 rqvaahtehl kealeeqkee lsrtftlele kkmveeraqh naalsssvak lqgmeeylra
      481 rdeldrlsqq akglwlacqg lrhtlvhgpa ggpqslstdv qlveraapms pyvplvlasi
      541 peealargvy pevalkerfa rveracqrva lvddkcpssl lrylasylqs lciiypkelp
      601 qeeltdeqrv npemwdtfdv lsrvrywlqh enleqalrya sqlrgqprlv aqdwihelrl
      661 hlearqaasa lmafaaatia edae
//
LOCUS       XP_077499009             682 aa            linear   INV 25-NOV-2025
DEFINITION  MICOS complex subunit MIC60-1-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077499009
VERSION     XP_077499009.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642883.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..682
                     /product="MICOS complex subunit MIC60-1-like isoform X3"
                     /calculated_mol_wt=76726
     Region          41..664
                     /region_name="Mitofilin"
                     /note="Mitochondrial inner membrane protein; pfam09731"
                     /db_xref="CDD:430783"
     CDS             1..682
                     /gene="LOC144110057"
                     /coded_by="XM_077642883.1:106..2154"
                     /db_xref="GeneID:144110057"
ORIGIN      
        1 mwrstrqvsw rcsrllpgnr hkarslvrlq stsssgrssa ktivitagva avgiggtlay
       61 akvdsdfrrk lessipygds llgsilgpaq gavrplpaev vkekalessl lrkrhpvepa
      121 rgdqrpalpp pppiavpppt pvkektpspq dleaeaqkql qdikernerl nfaleqslre
      181 aqaelekytk davvaeleak eaaqsyarkl yraideards edqymwdeal qakeaklaav
      241 qkaavkseqa wkclenlqsa ikkgqedket hsnlalspaq haakvaleql rnaesqsrsa
      301 keaartatdy kdaveraqlh fqkeieallp etkftgqkls edelnlllah ahrkienltk
      361 ahakiqvteq eraehfqrqq ervardldrq raeldamlev klvkqqeafe helqqqlrrq
      421 vaahtehlke aleeqkeels rtftlelekk mveeraqhna alsssvaklq gmeeylrard
      481 eldrlsqqak glwlacqglr htlvhgpagg pqslstdvql veraapmspy vplvlasipe
      541 ealargvype valkerfarv eracqrvalv ddkcpssllr ylasylqslc iiypkelpqe
      601 eltdeqrvnp emwdtfdvls rvrywlqhen leqalryasq lrgqprlvaq dwihelrlhl
      661 earqaasalm afaaatiaed ae
//
LOCUS       XP_077499018             146 aa            linear   INV 25-NOV-2025
DEFINITION  histone H2A.V isoform X1 [Amblyomma americanum].
ACCESSION   XP_077499018
VERSION     XP_077499018.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642892.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..146
                     /product="histone H2A.V isoform X1"
                     /calculated_mol_wt=15372
     Region          24..139
                     /region_name="PLN00154"
                     /note="histone H2A; Provisional"
                     /db_xref="CDD:177756"
     CDS             1..146
                     /gene="LOC144110076"
                     /coded_by="XM_077642892.1:60..500"
                     /db_xref="GeneID:144110076"
ORIGIN      
        1 metyascsta mfvaistena ngkagkdsgk akvkavsrse raglqfpvgr ihrylknrti
       61 shgrvgataa vysaavleyl taevlelagn askdmkvkri tprhlqlais gdeeldtlvr
      121 atiagggvvp hihksligkk gsqktl
//
LOCUS       XP_077499024             130 aa            linear   INV 25-NOV-2025
DEFINITION  histone H2A.V isoform X2 [Amblyomma americanum].
ACCESSION   XP_077499024
VERSION     XP_077499024.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642898.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..130
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..130
                     /product="histone H2A.V isoform X2"
                     /calculated_mol_wt=13635
     Region          8..123
                     /region_name="PLN00154"
                     /note="histone H2A; Provisional"
                     /db_xref="CDD:177756"
     CDS             1..130
                     /gene="LOC144110076"
                     /coded_by="XM_077642898.1:162..554"
                     /db_xref="GeneID:144110076"
ORIGIN      
        1 mvaangkagk dsgkakvkav srseraglqf pvgrihrylk nrtishgrvg ataavysaav
       61 leyltaevle lagnaskdmk vkritprhlq laisgdeeld tlvratiagg gvvphihksl
      121 igkkgsqktl
//
LOCUS       XP_077499030             128 aa            linear   INV 25-NOV-2025
DEFINITION  histone H2A.V isoform X3 [Amblyomma americanum].
ACCESSION   XP_077499030
VERSION     XP_077499030.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642904.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..128
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..128
                     /product="histone H2A.V isoform X3"
                     /calculated_mol_wt=13465
     Region          6..121
                     /region_name="PLN00154"
                     /note="histone H2A; Provisional"
                     /db_xref="CDD:177756"
     CDS             1..128
                     /gene="LOC144110076"
                     /coded_by="XM_077642904.1:162..548"
                     /db_xref="GeneID:144110076"
ORIGIN      
        1 mangkagkds gkakvkavsr seraglqfpv grihrylknr tishgrvgat aavysaavle
       61 yltaevlela gnaskdmkvk ritprhlqla isgdeeldtl vratiagggv vphihkslig
      121 kkgsqktl
//
LOCUS       XP_077499046             870 aa            linear   INV 25-NOV-2025
DEFINITION  AP-5 complex subunit beta-1-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077499046
VERSION     XP_077499046.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642920.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..870
                     /product="AP-5 complex subunit beta-1-like isoform X1"
                     /calculated_mol_wt=95632
     CDS             1..870
                     /gene="LOC144110102"
                     /coded_by="XM_077642920.1:138..2750"
                     /db_xref="GeneID:144110102"
ORIGIN      
        1 mgqlelwsnl aklrqtlyaf tgnevdtvgl hysdvvkvlm ddrqdeasql vmlvmlqeag
       61 syyrsgktay daveslksaf galkranryn aqsqtlvtmt tiilshesad caqlyeliel
      121 lvenvvqvkr nhplvraasc sclreielly pgsltkqleq lwgtfgkdyt wacqalalll
      181 vlggagpstl mgavsdenar slheasslvg ciplmtpvaa atmmrsllas ladglppall
      241 klccshfssc yelplvhlml smqgeackea wwqelwltiv eqladslhhp eswqalkvsw
      301 lgsvlrqqti gqslgpwhll ptprddvtqq glrleaalaa gfgawrgpeq mlgamipsvr
      361 clkrhvrlap gdfgihlllh vlyqlyadlr ntsaatsltv eitrlvinmt llgplyccsl
      421 lnflkcveag fpqsalsqvv lmeavdtvte qswedpvqgs tvescllvls aavqkpgilr
      481 qpkqlfhfiq qlvatqglsa qrtwalgnkl idlccsvmlh qtnhavssdl ctmlaylses
      541 pgdvdvksra kllyaalsal seskarqllq scslvpales ekgcnvlsdf pvpstvacpt
      601 mevlrlerml tegsqlqpcd qvpvevsehl siaalqryra flesefdqvv hipcrlfles
      661 vepqfdclmg lqlnlapped wgtaqgleas vfwknnsgvt tsvalfpqas vpcyitvrae
      721 ftsldscshv cwleplevrf edlllplpvp qplpaepqls vdsyramlwh slweqlsgkd
      781 clevqtsstl rlgpsppsqq lwlqlceala pfvvcwqprg lqaafvvppk mhvlvavgah
      841 gphlvaslvv edwnllpriq efllslctss
//
LOCUS       XP_077499054             831 aa            linear   INV 25-NOV-2025
DEFINITION  AP-5 complex subunit beta-1-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077499054
VERSION     XP_077499054.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642928.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..831
                     /product="AP-5 complex subunit beta-1-like isoform X2"
                     /calculated_mol_wt=91240
     CDS             1..831
                     /gene="LOC144110102"
                     /coded_by="XM_077642928.1:208..2703"
                     /db_xref="GeneID:144110102"
ORIGIN      
        1 mddrqdeasq lvmlvmlqea gsyyrsgkta ydaveslksa fgalkranry naqsqtlvtm
       61 ttiilshesa dcaqlyelie llvenvvqvk rnhplvraas csclreiell ypgsltkqle
      121 qlwgtfgkdy twacqalall lvlggagpst lmgavsdena rslheasslv gciplmtpva
      181 aatmmrslla sladglppal lklccshfss cyelplvhlm lsmqgeacke awwqelwlti
      241 veqladslhh peswqalkvs wlgsvlrqqt igqslgpwhl lptprddvtq qglrleaala
      301 agfgawrgpe qmlgamipsv rclkrhvrla pgdfgihlll hvlyqlyadl rntsaatslt
      361 veitrlvinm tllgplyccs llnflkcvea gfpqsalsqv vlmeavdtvt eqswedpvqg
      421 stvescllvl saavqkpgil rqpkqlfhfi qqlvatqgls aqrtwalgnk lidlccsvml
      481 hqtnhavssd lctmlaylse spgdvdvksr akllyaalsa lseskarqll qscslvpale
      541 sekgcnvlsd fpvpstvacp tmevlrlerm ltegsqlqpc dqvpvevseh lsiaalqryr
      601 aflesefdqv vhipcrlfle svepqfdclm glqlnlappe dwgtaqglea svfwknnsgv
      661 ttsvalfpqa svpcyitvra eftsldscsh vcwleplevr fedlllplpv pqplpaepql
      721 svdsyramlw hslweqlsgk dclevqtsst lrlgpsppsq qlwlqlceal apfvvcwqpr
      781 glqaafvvpp kmhvlvavga hgphlvaslv vedwnllpri qefllslcts s
//
LOCUS       XP_077499061             812 aa            linear   INV 25-NOV-2025
DEFINITION  AP-5 complex subunit beta-1-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077499061
VERSION     XP_077499061.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642935.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..812
                     /product="AP-5 complex subunit beta-1-like isoform X3"
                     /calculated_mol_wt=88518
     CDS             1..812
                     /gene="LOC144110102"
                     /coded_by="XM_077642935.1:120..2558"
                     /db_xref="GeneID:144110102"
ORIGIN      
        1 mgqlelwsnl aklrqtlyaf tgnevdtvgl hysdvvkvlm ddrqdeasql vmlvmlqeag
       61 syyrsgktay daveslksaf galkranryn aqsqtlvtmt tiilshesad caqlyeliel
      121 lvenvvqvkr nhplvraasc sclreielly pgsltkqleq lwgtfgkdyt wacqalalll
      181 vlggagpstl mgavsdenar slheasslvg ciplmtpvaa atmmrsllas ladglppall
      241 klccshfssc yelplvhlml smqgeackea wwqelwltiv eqladslhhp eswqalkvsw
      301 lgsvlrqqti gqslgpwhll ptprddvtqq glrleaalaa gfgawrgpeq mlgamipsvr
      361 clkrhvrlap gdfgihlllh vlyqlyadlr ntsaatsltv eitrlvinmt llgplyccsl
      421 lnflkcveag fpqsalsqvv lmeavdtvte qswedpvqgs tvescllvls aavqkpgilr
      481 qpkqlfhfiq qlvatqglsa qrtwalgnkl idlccsvmlh qtnhavssdl ctmlaylses
      541 pgdvdvksra kllyaalsal seskarqllq scslvpales ekgcnvlsdf pvpstvacpt
      601 mevlrlerml tegsqlqpcd qvpvevsehl siaalqryra flesefdqvv hipcrlfles
      661 vepqfdclmg lqlnlapped wgtaqgleas vfwknnsgvt tsvalfpqas vpcyitvrae
      721 ftsldscshv cwleplevkw kglprssdiv dpapwtqpsq satlaaavrs pgsicgvlaa
      781 trtagsicra pkdacfggrg ctwttpccfi gg
//
LOCUS       XP_077499073             189 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144110121 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077499073
VERSION     XP_077499073.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642947.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..189
                     /product="uncharacterized protein LOC144110121 isoform X1"
                     /calculated_mol_wt=20171
     CDS             1..189
                     /gene="LOC144110121"
                     /coded_by="XM_077642947.1:152..721"
                     /db_xref="GeneID:144110121"
ORIGIN      
        1 mysflaveld eifekahsin ppyllgpglv rsiqdsqaep vaspqlgpsp tvtaspipgs
       61 sqpstsrgst tagdsqppsp smlpsprqqq pgparkrprt dtshamlesa vthlevaean
      121 rqqghrermt leerkvaaee rtaalervaa alerqalvsl yspirapahs pthssihspi
      181 rslskpsll
//
LOCUS       XP_077499079             188 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144110121 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077499079
VERSION     XP_077499079.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642953.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..188
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..188
                     /product="uncharacterized protein LOC144110121 isoform X2"
                     /calculated_mol_wt=20084
     CDS             1..188
                     /gene="LOC144110121"
                     /coded_by="XM_077642953.1:152..718"
                     /db_xref="GeneID:144110121"
ORIGIN      
        1 mysflaveld eifekahsin ppyllgpglv rsiqdqaepv aspqlgpspt vtaspipgss
       61 qpstsrgstt agdsqppsps mlpsprqqqp gparkrprtd tshamlesav thlevaeanr
      121 qqghrermtl eerkvaaeer taalervaaa lerqalvsly spirapahsp thssihspir
      181 slskpsll
//
LOCUS       XP_077499096             351 aa            linear   INV 25-NOV-2025
DEFINITION  ADP-ribosylhydrolase ARH3-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077499096
VERSION     XP_077499096.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642970.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..351
                     /product="ADP-ribosylhydrolase ARH3-like isoform X1"
                     /calculated_mol_wt=37776
     Region          14..351
                     /region_name="DraG"
                     /note="ADP-ribosylglycohydrolase [Posttranslational
                     modification, protein turnover, chaperones]; COG1397"
                     /db_xref="CDD:441007"
     CDS             1..351
                     /gene="LOC144110139"
                     /coded_by="XM_077642970.1:223..1278"
                     /db_xref="GeneID:144110139"
ORIGIN      
        1 msavansvsl lagkfrgclv gsllgdclga pfegdfpvsk svltsyiskl ldesakgllp
       61 frpytddtam tkclaaslie kkgfhagdla krftteyfeq pkrgygpnvv dvfhalkknn
      121 fedpfgpakd qfqgtgsygn ggamrvapva lfwhrnlaem vsvaekqari ththrngcng
      181 avlqclavyr allsdgqapl nkaaflefll ermdaiesrd vkpvsdpavc kpfttslqnm
      241 rrvlcddpqd lsaeeiaalf gndvsahksv ptaiyaflrs qeplpffqae npfvraivls
      301 isvggdtdti asmagsisga fhgiagipip lqrhcegldi tlkladdlyn l
//
LOCUS       XP_077499106             297 aa            linear   INV 25-NOV-2025
DEFINITION  ADP-ribosylhydrolase ARH3-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077499106
VERSION     XP_077499106.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642980.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..297
                     /product="ADP-ribosylhydrolase ARH3-like isoform X2"
                     /calculated_mol_wt=32167
     Region          14..>192
                     /region_name="DraG"
                     /note="ADP-ribosylglycohydrolase [Posttranslational
                     modification, protein turnover, chaperones]; COG1397"
                     /db_xref="CDD:441007"
     CDS             1..297
                     /gene="LOC144110139"
                     /coded_by="XM_077642980.1:210..1103"
                     /db_xref="GeneID:144110139"
ORIGIN      
        1 msavansvsl lagkfrgclv gsllgdclga pfegdfpvsk svltsyiskl ldesakgllp
       61 frpytddtam tkclaaslie kkgfhagdla krftteyfeq pkrgygpnvv dvfhalkknn
      121 fedpfgpakd qfqgtgsygn ggamrvapva lfwhrnlaem vsvaekqari ththrngcng
      181 avlqclavyr allsdgqapl nkaaflefll ermdaiesrd vkpvsdpavc kpfttslqnm
      241 rrvlcddpqd lsaeeiaalf gndvsahksv ptaiyaflrs qeplpffqtf kgasavf
//
LOCUS       XP_077499115            1075 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylinositide phosphatase spermathreecae [Amblyomma
            americanum].
ACCESSION   XP_077499115
VERSION     XP_077499115.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642989.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1075
                     /product="phosphatidylinositide phosphatase
                     spermathreecae"
                     /calculated_mol_wt=120907
     Region          152..571
                     /region_name="COG5329"
                     /note="Phosphoinositide polyphosphatase (Sac family)
                     [Signal transduction mechanisms]"
                     /db_xref="CDD:227637"
     Region          593..698
                     /region_name="hSac2"
                     /note="Inositol phosphatase; pfam12456"
                     /db_xref="CDD:463592"
     CDS             1..1075
                     /gene="sp3"
                     /coded_by="XM_077642989.1:229..3456"
                     /db_xref="GeneID:144110149"
ORIGIN      
        1 meilmtpdyy vivdgeetlw csridgklep rkrselqqlt davclgtvyg iigkfqphpd
       61 sdqrlvlirq msligslpgn hqvfkvnkvv lvplsiheap elemepctkh qsswrrsers
      121 paselqqral qktwqtikta taqvkprrtv qsrerekyer rlldellkmf ndtdsfyfsp
      181 tgdltntmqr qveqppllag sprwerlddr ffwnremlrd lteteggehw vlpiiqgfvq
      241 vercqldpid sgqpeaalgq dssgtwfdvq svarppakef tmslvsrrsr yragtrykrr
      301 gvdemgkcan yveteqifeh aahvvsfvqv rgsvpvfwsq pgykyrpppc ldrdeeetkq
      361 afakhfaeqv alygsqvivs lveqtgkekl ladaylnhvl hldcpaltyv sfdfheycrg
      421 mrfenvsvli dgikdlirdm rycwldqqgl icaqrgvfrv ncidcldrtn ivqtaiakta
      481 mdtqfvrlgl lppegvlpta crrifqmlwa nngdiisrqy agtvalkgdf trtgsrrlag
      541 mmkdgynsan ryyvnrfkda yrqatidlml gnpitedfma vspeqddepv lemqdqehqe
      601 hvkqliehck kmlipesevv lggwalidad pvtgdpgqad mdsilvltkd syyvaeyddq
      661 tdritkyqrv fledlekmel gpepslfksr hycirfhylv ngqsgyfhmf rstgtrffnn
      721 vavpisgeee tieslkaice sfkvalsvks lsvplfegkl errrsklpfv qgerpgslgl
      781 aplgvmtrnv segqlmafvs vgsralsnvt sqfarlnpmr svrglakrpt talgaltegs
      841 fqvdsssdse edsarwlrqp lpaaertlss dyssdsdape tghdallesc gilatspsla
      901 nceprppthq aldiddfvld amhraslrqh kapaaavapq iqvsgespae apsggrlrql
      961 sksseelesr panmlpgnrl fvleegprep akmkmshses aiqdfslpgp spvsspiiik
     1021 kdlvlsplsr iakgvqtlgl nlrpaaivsr raplpvesea lqqrkracrs hilel
//
LOCUS       XP_077499124             329 aa            linear   INV 25-NOV-2025
DEFINITION  casein kinase Ialpha [Amblyomma americanum].
ACCESSION   XP_077499124
VERSION     XP_077499124.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077642998.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..329
                     /product="casein kinase Ialpha"
                     /calculated_mol_wt=37578
     Region          19..284
                     /region_name="STKc_CK1_alpha"
                     /note="Catalytic domain of the Serine/Threonine protein
                     kinases, Casein Kinase 1 alpha; cd14128"
                     /db_xref="CDD:271030"
     Site            order(26..32,34,47,49,93..97,99,101,139,141,143..144,146,
                     160,163,186..189)
                     /site_type="active"
                     /db_xref="CDD:271030"
     Site            order(26..32,34,47,49,93..97,141,143..144,146,160)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271030"
     Site            order(30,99,101,139,141,143,163,186..189)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271030"
     Site            159..189
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271030"
     CDS             1..329
                     /gene="CkIalpha"
                     /coded_by="XM_077642998.1:124..1113"
                     /db_xref="GeneID:144110153"
ORIGIN      
        1 mamasaagss raefivggkf klvrkvgsgs fgdiylginm vngeevavkm estkarhpql
       61 lyesklykil qghegiphtr wfgterdynv lvmdllgpsl edlfnfcsrr ftmktvlmlf
      121 dqmithieyv hnknfihrdi kpdnflmgig ghcnklflid fglakkyrdn rnkqhipyre
      181 dknltgtary asinahlgie qsrrddmesm gyvlmyfnrg slpwqglkaa tkkqkyekis
      241 ekkmstpvev lckgfpaeft mylnycrglr feempdykyl rqifrilfrt lnhqydyvfd
      301 wtmlkqkaaa saaasgamag qpgtaaggr
//
LOCUS       XP_077499143            1017 aa            linear   INV 25-NOV-2025
DEFINITION  insulin degrading metalloproteinase isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077499143
VERSION     XP_077499143.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643017.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1017
                     /product="insulin degrading metalloproteinase isoform X1"
                     /calculated_mol_wt=115997
     Region          57..998
                     /region_name="Ptr"
                     /note="Secreted/periplasmic Zn-dependent peptidases,
                     insulinase-like [Posttranslational modification, protein
                     turnover, chaperones]; COG1025"
                     /db_xref="CDD:440648"
     CDS             1..1017
                     /gene="Ide"
                     /coded_by="XM_077643017.1:220..3273"
                     /db_xref="GeneID:144110179"
ORIGIN      
        1 mfvrsrwscf lgqravstlr kcysscilpg qrpiavssrq ahmmpsidad hgarvhnnit
       61 qsesdknlyr glelrngmkv llisdpttdk saaalnvqvg ymsdpwelpg lahfcehmlf
      121 lgtekypsen eyhkylcqha gssnaftasd htcyyfdvap enlepaldrf aaffvcplfn
      181 edatdrevna ihsehiknvq ndswrlkqle astadpkhdf skfgsgnktt ldaipkskgl
      241 mvrdellkfh qqwyssnims lvvlgkesld dlarmvvplf svvpdkgvep ptwpqhpygr
      301 eqlglrarvv pvkdnrflhl tfptpdlrqy yragpgdyva hlighegpgs llselkargw
      361 vnslvggekd gargfsftiv nvdlteegme htddivqlvf qylsmlrneg pqkwifqelq
      421 elwrvafrfk gkdtpqsyvr dlsgmlhylp fqdvlagpyi ideyrpdlik dllgylrpen
      481 vrvavvakhf tgqtdsvekw ygtlykldri peavmqvwqe pgvnknlklp prnefipsnf
      541 dqcprepege qlpvmiknte gtrvwfvqdh tynlpkavlh fefkspvayq dphhtnmthm
      601 fvrlftdaln eytyaamqag lsysldntiy givlsirgyn dkqhvllaki mdkmtnfvvd
      661 qqrfdilrea yvrglknfta epphqhavyy tymllaqkvw shnemleate eltkervqem
      721 ipkllsrmhi eclmhgnltr qhaldlvgiv egslqasvgt kpllpselvg hrehqllerg
      781 eyvyeqvnqv hhtssiqtyf qcgpqetran mlvellcqli tepcynilrt qeqlgylvas
      841 gprrsngvqg iriivqsdrp plfldsriea flvyieeyiq nmsdeefgfn ktalaarrle
      901 kpkklsqman kywmeilsqq ynfdrdaiev aslegltsad lltffkehia agapfrkkls
      961 vhikccgqgd ttddtsptng pmwiknvtef krslglyplp kacinvqppq sgakskl
//
LOCUS       XP_077499145             975 aa            linear   INV 25-NOV-2025
DEFINITION  insulin degrading metalloproteinase isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077499145
VERSION     XP_077499145.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643019.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..975
                     /product="insulin degrading metalloproteinase isoform X2"
                     /calculated_mol_wt=111230
     Region          15..956
                     /region_name="Ptr"
                     /note="Secreted/periplasmic Zn-dependent peptidases,
                     insulinase-like [Posttranslational modification, protein
                     turnover, chaperones]; COG1025"
                     /db_xref="CDD:440648"
     CDS             1..975
                     /gene="Ide"
                     /coded_by="XM_077643019.1:429..3356"
                     /db_xref="GeneID:144110179"
ORIGIN      
        1 mmpsidadhg arvhnnitqs esdknlyrgl elrngmkvll isdpttdksa aalnvqvgym
       61 sdpwelpgla hfcehmlflg tekypseney hkylcqhags snaftasdht cyyfdvapen
      121 lepaldrfaa ffvcplfned atdrevnaih sehiknvqnd swrlkqleas tadpkhdfsk
      181 fgsgnkttld aipkskglmv rdellkfhqq wyssnimslv vlgkeslddl armvvplfsv
      241 vpdkgveppt wpqhpygreq lglrarvvpv kdnrflhltf ptpdlrqyyr agpgdyvahl
      301 ighegpgsll selkargwvn slvggekdga rgfsftivnv dlteegmeht ddivqlvfqy
      361 lsmlrnegpq kwifqelqel wrvafrfkgk dtpqsyvrdl sgmlhylpfq dvlagpyiid
      421 eyrpdlikdl lgylrpenvr vavvakhftg qtdsvekwyg tlykldripe avmqvwqepg
      481 vnknlklppr nefipsnfdq cprepegeql pvmikntegt rvwfvqdhty nlpkavlhfe
      541 fkspvayqdp hhtnmthmfv rlftdalney tyaamqagls ysldntiygi vlsirgyndk
      601 qhvllakimd kmtnfvvdqq rfdilreayv rglknftaep phqhavyyty mllaqkvwsh
      661 nemleateel tkervqemip kllsrmhiec lmhgnltrqh aldlvgiveg slqasvgtkp
      721 llpselvghr ehqllergey vyeqvnqvhh tssiqtyfqc gpqetranml vellcqlite
      781 pcynilrtqe qlgylvasgp rrsngvqgir iivqsdrppl fldsrieafl vyieeyiqnm
      841 sdeefgfnkt alaarrlekp kklsqmanky wmeilsqqyn fdrdaievas legltsadll
      901 tffkehiaag apfrkklsvh ikccgqgdtt ddtsptngpm wiknvtefkr slglyplpka
      961 cinvqppqsg akskl
//
LOCUS       XP_077499153             975 aa            linear   INV 25-NOV-2025
DEFINITION  insulin degrading metalloproteinase isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077499153
VERSION     XP_077499153.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643027.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..975
                     /product="insulin degrading metalloproteinase isoform X2"
                     /calculated_mol_wt=111230
     Region          15..956
                     /region_name="Ptr"
                     /note="Secreted/periplasmic Zn-dependent peptidases,
                     insulinase-like [Posttranslational modification, protein
                     turnover, chaperones]; COG1025"
                     /db_xref="CDD:440648"
     CDS             1..975
                     /gene="Ide"
                     /coded_by="XM_077643027.1:504..3431"
                     /db_xref="GeneID:144110179"
ORIGIN      
        1 mmpsidadhg arvhnnitqs esdknlyrgl elrngmkvll isdpttdksa aalnvqvgym
       61 sdpwelpgla hfcehmlflg tekypseney hkylcqhags snaftasdht cyyfdvapen
      121 lepaldrfaa ffvcplfned atdrevnaih sehiknvqnd swrlkqleas tadpkhdfsk
      181 fgsgnkttld aipkskglmv rdellkfhqq wyssnimslv vlgkeslddl armvvplfsv
      241 vpdkgveppt wpqhpygreq lglrarvvpv kdnrflhltf ptpdlrqyyr agpgdyvahl
      301 ighegpgsll selkargwvn slvggekdga rgfsftivnv dlteegmeht ddivqlvfqy
      361 lsmlrnegpq kwifqelqel wrvafrfkgk dtpqsyvrdl sgmlhylpfq dvlagpyiid
      421 eyrpdlikdl lgylrpenvr vavvakhftg qtdsvekwyg tlykldripe avmqvwqepg
      481 vnknlklppr nefipsnfdq cprepegeql pvmikntegt rvwfvqdhty nlpkavlhfe
      541 fkspvayqdp hhtnmthmfv rlftdalney tyaamqagls ysldntiygi vlsirgyndk
      601 qhvllakimd kmtnfvvdqq rfdilreayv rglknftaep phqhavyyty mllaqkvwsh
      661 nemleateel tkervqemip kllsrmhiec lmhgnltrqh aldlvgiveg slqasvgtkp
      721 llpselvghr ehqllergey vyeqvnqvhh tssiqtyfqc gpqetranml vellcqlite
      781 pcynilrtqe qlgylvasgp rrsngvqgir iivqsdrppl fldsrieafl vyieeyiqnm
      841 sdeefgfnkt alaarrlekp kklsqmanky wmeilsqqyn fdrdaievas legltsadll
      901 tffkehiaag apfrkklsvh ikccgqgdtt ddtsptngpm wiknvtefkr slglyplpka
      961 cinvqppqsg akskl
//
LOCUS       XP_077499168             206 aa            linear   INV 25-NOV-2025
DEFINITION  serine/arginine-rich splicing factor 12-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077499168
VERSION     XP_077499168.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643042.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..206
                     /product="serine/arginine-rich splicing factor 12-like
                     isoform X1"
                     /calculated_mol_wt=24575
     Region          11..94
                     /region_name="RRM_SRSF10_SRSF12"
                     /note="RNA recognition motif (RRM) found in
                     serine/arginine-rich splicing factor SRSF10, SRSF12 and
                     similar proteins; cd12312"
                     /db_xref="CDD:240758"
     CDS             1..206
                     /gene="LOC144110200"
                     /coded_by="XM_077643042.1:92..712"
                     /db_xref="GeneID:144110200"
ORIGIN      
        1 mssrysrppn sslfirnvpd gtrpddlrsl fgkygpltdv yipvdyytrr prgfayvqfe
       61 dlrdaedamy sldrtrfygr eleiefaqgd rktpsemrgr ermrrtrspy ghgrhdergr
      121 rrsrsrspya rdrsprrsrr hsysrsrsrs rdrrrqsysp rrsrsysrsr srsqsshlgs
      181 kgksrdrspy eiidesreks rskspe
//
LOCUS       XP_077499172             180 aa            linear   INV 25-NOV-2025
DEFINITION  serine/arginine-rich splicing factor 12-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077499172
VERSION     XP_077499172.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643046.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..180
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..180
                     /product="serine/arginine-rich splicing factor 12-like
                     isoform X2"
                     /calculated_mol_wt=21200
     Region          11..94
                     /region_name="RRM_SRSF10_SRSF12"
                     /note="RNA recognition motif (RRM) found in
                     serine/arginine-rich splicing factor SRSF10, SRSF12 and
                     similar proteins; cd12312"
                     /db_xref="CDD:240758"
     CDS             1..180
                     /gene="LOC144110200"
                     /coded_by="XM_077643046.1:95..637"
                     /db_xref="GeneID:144110200"
ORIGIN      
        1 mssrysrppn sslfirnvpd gtrpddlrsl fgkygpltdv yipvdyytrr prgfayvqfe
       61 dlrdaedamy sldrtrfygr eleiefaqgd rktpsemrgr ermrrtrspy ghgrhdergr
      121 rrsrsrspya rdrsprsrsy srsrsrsqss hlgskgksrd rspyeiides reksrskspe
//
LOCUS       XP_077499181             750 aa            linear   INV 25-NOV-2025
DEFINITION  suv3 RNA helicase isoform X1 [Amblyomma americanum].
ACCESSION   XP_077499181
VERSION     XP_077499181.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643055.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..750
                     /product="suv3 RNA helicase isoform X1"
                     /calculated_mol_wt=84422
     Region          76..188
                     /region_name="Suv3_N"
                     /note="Suv3 helical N-terminal domain; pfam18114"
                     /db_xref="CDD:436283"
     Region          209..354
                     /region_name="DEXQc_Suv3"
                     /note="DEXQ-box helicase domain of Suv3; cd17913"
                     /db_xref="CDD:350671"
     Site            order(218..224,300)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350671"
     Region          358..496
                     /region_name="SF2_C_suv3"
                     /note="C-terminal helicase domain of ATP-dependent RNA
                     helicase; cd18805"
                     /db_xref="CDD:350192"
     Site            order(438,441,475,479,482..483)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350192"
     Region          568..608
                     /region_name="Suv3_C_1"
                     /note="Suv3 C-terminal domain 1; pfam18147"
                     /db_xref="CDD:436308"
     Region          636..682
                     /region_name="SUV3_C"
                     /note="Mitochondrial degradasome RNA helicase subunit C
                     terminal; pfam12513"
                     /db_xref="CDD:463616"
     CDS             1..750
                     /gene="Suv3"
                     /coded_by="XM_077643055.1:160..2412"
                     /db_xref="GeneID:144110214"
ORIGIN      
        1 mpgskmhlvk lsilgtmcrg ttrtfggtlw qirfrmvape tlhlrslprr lqyvnfvisi
       61 rhksskssvs slfmpvpvkl snnpddinvg eelttklkke dllkllnqfs rrpevvkwse
      121 engldarlfh qafvsfrryc meseqlpadl hivfsdllqg grhlddlvpy flhhakqifp
      181 hlecmeelqk isdlrlpanw ypearavqrk iifhagptns gkthaalerf qtansglycg
      241 plkmlavevf qktnekgtpc dlvtgeerry alpdgqpaph vactvematv htpydvavid
      301 eiqmmrdpqr gwawtrallg laakeihlcg eaaavglvrt lltslgeele vrkykrltql
      361 vvenralesl ekiqpgdcvv cfsksdiyqv slqierqgle caviygglpp gtklaqaqkf
      421 ndpahpckvl vatdaigmgl nlsigrvifh slvkpslner gerqldtiss sqalqiagra
      481 grfgsrfevg rvttmksqdl palkqilaap veqieaaglh ptaeqielfa yhlphatlan
      541 lvdifvslck vdsssyfmcn legfkfladm iqhvplplra ryvfccspin qkmpfvcsmf
      601 lkfarqysrn elltcqwlgr nigwplaipk simdlvhlea vfdvldlylw lsyrfpdlfp
      661 daeavramqq eldliiqkgv lnitqllrag mdsaaeeaer ddrptsapkl qgkladrlla
      721 qglltkeqll elqrewqrts pkdrrskkpk
//
LOCUS       XP_077499187             624 aa            linear   INV 25-NOV-2025
DEFINITION  suv3 RNA helicase isoform X2 [Amblyomma americanum].
ACCESSION   XP_077499187
VERSION     XP_077499187.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643061.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..624
                     /product="suv3 RNA helicase isoform X2"
                     /calculated_mol_wt=69973
     Region          76..188
                     /region_name="Suv3_N"
                     /note="Suv3 helical N-terminal domain; pfam18114"
                     /db_xref="CDD:436283"
     Region          209..354
                     /region_name="DEXQc_Suv3"
                     /note="DEXQ-box helicase domain of Suv3; cd17913"
                     /db_xref="CDD:350671"
     Site            order(218..224,300)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350671"
     Region          358..496
                     /region_name="SF2_C_suv3"
                     /note="C-terminal helicase domain of ATP-dependent RNA
                     helicase; cd18805"
                     /db_xref="CDD:350192"
     Site            order(438,441,475,479,482..483)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350192"
     Region          568..608
                     /region_name="Suv3_C_1"
                     /note="Suv3 C-terminal domain 1; pfam18147"
                     /db_xref="CDD:436308"
     CDS             1..624
                     /gene="Suv3"
                     /coded_by="XM_077643061.1:158..2032"
                     /db_xref="GeneID:144110214"
ORIGIN      
        1 mpgskmhlvk lsilgtmcrg ttrtfggtlw qirfrmvape tlhlrslprr lqyvnfvisi
       61 rhksskssvs slfmpvpvkl snnpddinvg eelttklkke dllkllnqfs rrpevvkwse
      121 engldarlfh qafvsfrryc meseqlpadl hivfsdllqg grhlddlvpy flhhakqifp
      181 hlecmeelqk isdlrlpanw ypearavqrk iifhagptns gkthaalerf qtansglycg
      241 plkmlavevf qktnekgtpc dlvtgeerry alpdgqpaph vactvematv htpydvavid
      301 eiqmmrdpqr gwawtrallg laakeihlcg eaaavglvrt lltslgeele vrkykrltql
      361 vvenralesl ekiqpgdcvv cfsksdiyqv slqierqgle caviygglpp gtklaqaqkf
      421 ndpahpckvl vatdaigmgl nlsigrvifh slvkpslner gerqldtiss sqalqiagra
      481 grfgsrfevg rvttmksqdl palkqilaap veqieaaglh ptaeqielfa yhlphatlan
      541 lvdifvslck vdsssyfmcn legfkfladm iqhvplplra ryvfccspin qkmpfvcsmf
      601 lkfarqysrn elltcqwlgr nnlp
//
LOCUS       XP_077499197             430 aa            linear   INV 25-NOV-2025
DEFINITION  ornithine aminotransferase precursor [Amblyomma americanum].
ACCESSION   XP_077499197
VERSION     XP_077499197.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643071.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..430
                     /product="ornithine aminotransferase precursor"
                     /calculated_mol_wt=47059
     Region          30..424
                     /region_name="Orn_aminotrans"
                     /note="ornithine aminotransferase; TIGR01885"
                     /db_xref="CDD:273853"
     Site            order(131..133,167..168,170,220,253,255..256,282)
                     /site_type="active"
                     /note="inhibitor-cofactor binding pocket [active]"
                     /db_xref="CDD:99735"
     Site            order(132..133,167..168,220,253,256,282)
                     /site_type="other"
                     /note="pyridoxal 5'-phosphate binding site [chemical
                     binding]"
                     /db_xref="CDD:99735"
     Site            282
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:99735"
     CDS             1..430
                     /gene="Oat"
                     /coded_by="XM_077643071.1:241..1533"
                     /db_xref="GeneID:144110228"
ORIGIN      
        1 mlaakaskil rqqlswlqkr tqtklyplss eqvferedky gahnyhplpv aihkakgvhv
       61 wdvegriyfd flsaysavnq ghchpkivka lkdqaevltl tsrafyntal geleerltkm
      121 fgydkvlpmn tgveaaetac klarrwgydv kgipankaki vfaennfwgr slaavsastd
      181 pssyagfgpf ipgfeiipyd dlsaleravq dphvcafmve piqgeagvvv pspgylrgvr
      241 dictkhrvlw iadeiqtglg rtgkmlcvdh dnvrpdivtl gkalsgglyp vsaalandev
      301 mlvikpgehg styggnplga rvalaalevl eeeklaenaq ylgeilrakl nrlpkdlviq
      361 vrgkgllnai ivapgidawe vclrlkengv lakpthgdki rlapplvite kelcqcidii
      421 gdtitsfkpm
//
LOCUS       XP_077499202             430 aa            linear   INV 25-NOV-2025
DEFINITION  ornithine aminotransferase precursor [Amblyomma americanum].
ACCESSION   XP_077499202
VERSION     XP_077499202.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643076.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..430
                     /product="ornithine aminotransferase precursor"
                     /calculated_mol_wt=47059
     Region          30..424
                     /region_name="Orn_aminotrans"
                     /note="ornithine aminotransferase; TIGR01885"
                     /db_xref="CDD:273853"
     Site            order(131..133,167..168,170,220,253,255..256,282)
                     /site_type="active"
                     /note="inhibitor-cofactor binding pocket [active]"
                     /db_xref="CDD:99735"
     Site            order(132..133,167..168,220,253,256,282)
                     /site_type="other"
                     /note="pyridoxal 5'-phosphate binding site [chemical
                     binding]"
                     /db_xref="CDD:99735"
     Site            282
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:99735"
     CDS             1..430
                     /gene="Oat"
                     /coded_by="XM_077643076.1:175..1467"
                     /db_xref="GeneID:144110228"
ORIGIN      
        1 mlaakaskil rqqlswlqkr tqtklyplss eqvferedky gahnyhplpv aihkakgvhv
       61 wdvegriyfd flsaysavnq ghchpkivka lkdqaevltl tsrafyntal geleerltkm
      121 fgydkvlpmn tgveaaetac klarrwgydv kgipankaki vfaennfwgr slaavsastd
      181 pssyagfgpf ipgfeiipyd dlsaleravq dphvcafmve piqgeagvvv pspgylrgvr
      241 dictkhrvlw iadeiqtglg rtgkmlcvdh dnvrpdivtl gkalsgglyp vsaalandev
      301 mlvikpgehg styggnplga rvalaalevl eeeklaenaq ylgeilrakl nrlpkdlviq
      361 vrgkgllnai ivapgidawe vclrlkengv lakpthgdki rlapplvite kelcqcidii
      421 gdtitsfkpm
//
LOCUS       XP_077499212             443 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylinositol 4-kinase II alpha isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077499212
VERSION     XP_077499212.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643086.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="phosphatidylinositol 4-kinase II alpha isoform
                     X1"
                     /calculated_mol_wt=50750
     Region          96..387
                     /region_name="PI3_PI4_kinase"
                     /note="Phosphatidylinositol 3- and 4-kinase; pfam00454"
                     /db_xref="CDD:395364"
     CDS             1..443
                     /gene="Pi4KIIalpha"
                     /coded_by="XM_077643086.1:106..1437"
                     /db_xref="GeneID:144110246"
ORIGIN      
        1 mtseqgklra inagddpgly qdsdeqnlpd vtfsvgtsla snasrtresq pllrrmepdl
       61 gttaetfpdd pqfaavvrea ftaiesgiyp eriyqgssgs yfvkncertk iavykpkdee
      121 pygrlnpkwt kwmhklccpc cfgrsclvpn qgylseagas lvdqklqlni vpktkvvklv
      181 setfnysald rakartkknv terfpkvgrh fhriglphkv gsfqlyvdgy qdadywlrkf
      241 eseplppelq rifqfqferl vvldyiirnt drgndnwlik ctrpdkepqs wsprtpeiki
      301 aaidnglafp fkhpdswray pyhwawlpma kvpfstetrd lvlpqlsdmn fvqemcdelh
      361 alfkkdpgfd knlfekqmsv mrgqilnlnq alkdgkspvq lvqmpavime rssrrpgarg
      421 rikslndnfi qsfqnkapff swc
//
LOCUS       XP_077499217             430 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylinositol 4-kinase II alpha isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077499217
VERSION     XP_077499217.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643091.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..430
                     /product="phosphatidylinositol 4-kinase II alpha isoform
                     X2"
                     /calculated_mol_wt=49214
     Region          96..374
                     /region_name="PI3_PI4_kinase"
                     /note="Phosphatidylinositol 3- and 4-kinase; pfam00454"
                     /db_xref="CDD:395364"
     CDS             1..430
                     /gene="Pi4KIIalpha"
                     /coded_by="XM_077643091.1:108..1400"
                     /db_xref="GeneID:144110246"
ORIGIN      
        1 mtseqgklra inagddpgly qdsdeqnlpd vtfsvgtsla snasrtresq pllrrmepdl
       61 gttaetfpdd pqfaavvrea ftaiesgiyp eriyqgssgs yfvkncertk iavykpkdee
      121 pygrlnpkwt kwmhklccpc cfgrsclvpn qgylseagas lvdqklqlni vpktkvvklv
      181 setfnysald rakartkknv terfpkvgsf qlyvdgyqda dywlrkfese plppelqrif
      241 qfqferlvvl dyiirntdrg ndnwlikctr pdkepqswsp rtpeikiaai dnglafpfkh
      301 pdswraypyh wawlpmakvp fstetrdlvl pqlsdmnfvq emcdelhalf kkdpgfdknl
      361 fekqmsvmrg qilnlnqalk dgkspvqlvq mpavimerss rrpgargrik slndnfiqsf
      421 qnkapffswc
//
LOCUS       XP_077499233            1169 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: transcription elongation regulator 1-like
            [Amblyomma americanum].
ACCESSION   XP_077499233
VERSION     XP_077499233.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643107.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1169
                     /product="LOW QUALITY PROTEIN: transcription elongation
                     regulator 1-like"
                     /calculated_mol_wt=127980
     Region          216..241
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          481..1131
                     /region_name="PRP40"
                     /note="Splicing factor [RNA processing and modification];
                     COG5104"
                     /db_xref="CDD:227435"
     CDS             1..1169
                     /gene="LOC144110263"
                     /coded_by="XM_077643107.1:99..3608"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:144110263"
ORIGIN      
        1 mgdsnfemsd gdgpndvvkg gfmhrnrgtd gfpprgppsf gprgppprfr gpppgmmgrr
       61 gpppmmrpmm grppppgfrp pfdpsfpppp nmgppppmgm gpppgmagpp ppnfqnggea
      121 fggmppprpp lmpppgfgmg ppphqqqhpp qqgpppmsgp ppmggpppsl spppgmpppp
      181 atsmhappat gamalappqg apppqqpcld lsgeiwvetk naqgksyyyn artresawnk
      241 pegpgvkvls qeqiesmava agvapggnaa aaaataastp aaaappvtst tspatsvqvv
      301 tsssapptpq piatgsmesq tadkpptsta qggeqpaaap aaattappgp appaiagsgd
      361 pppdrpsaqq qqqetpkgvp ppspgslstl vqpplavpap tfslpppfgl ppppfgmppv
      421 pgvvppgfpn lglppppfgp plglppfvtp vpspaalppl qvatpatatp aavptgesel
      481 reraaqwteh kttdgksyyh nmrtqqstwd rpealveldk alatgapdgs gsgsadatka
      541 vvnpasgesr stadpaiada aqadkpavtk sieggqaaap vkrppakpqd ktkpvsstpv
      601 pgtpwcvvwt gdnrvfffnp strtsvwerp aelkkrtdvd kmvqtppvqp evkgqdasak
      661 dgeppskktk teeveesaae gteaqngate akteepatav lrgqgkesam eaelraaker
      721 aaipldvrmq rfrdmlveke vsafstweke lhkivfdsry llltskerkq vfekyvkera
      781 eeerrekrnk mrerkdhfqq llesaalnsk stfsdfaqky gkderfknie kmreresmfn
      841 dyvqelrkle reerlnqrek mkkdflellk eqkhldkhsr wndvkksmve daryrsvdss
      901 sqreewfkey vskltaahgh egedessrer ekqerieasl rerekevqrt lsthlrerdk
      961 ereqhkhdea vqhfnalltd lvrnpdaswr eakrtlrkdh rwdlvesler eereklfteh
     1021 veqlqrkkkd kyrdlldeta gitlsstwke vkklirddpr yakfssserk cekefkeylk
     1081 dkmaaaksdf rellketkti tykskkqiee seqhlldiqk vlekdkrylv lscipderrk
     1141 llmayvedld rrgpppppta septrrsnk
//
LOCUS       XP_077499258             180 aa            linear   INV 25-NOV-2025
DEFINITION  DNA polymerase epsilon subunit 3 Chrac-14 [Amblyomma americanum].
ACCESSION   XP_077499258
VERSION     XP_077499258.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643132.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..180
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..180
                     /product="DNA polymerase epsilon subunit 3 Chrac-14"
                     /calculated_mol_wt=17978
     Region          6..92
                     /region_name="HFD_POLE3_DPB4"
                     /note="histone-fold domain found in DNA polymerase epsilon
                     subunit 3 (POLE3) and similar proteins; cd22928"
                     /db_xref="CDD:467053"
     Site            order(6..7,39,43,46..47,50..51,66..67,75,80,86..87,90)
                     /site_type="other"
                     /note="catalytic subunit binding site [polypeptide
                     binding]"
                     /db_xref="CDD:467053"
     Site            order(14,18..19,21,29..30,32..33,36..37,39..41,43..49,
                     62..66,69,76..78,81,84..85,88..89,91..92)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467053"
     CDS             1..180
                     /gene="Chrac-14"
                     /coded_by="XM_077643132.1:224..766"
                     /db_xref="GeneID:144110288"
ORIGIN      
        1 maerpedlnl plsvvtrivk dalpdgvnvs kdaraalaka asvfvlytts cannfamkgk
       61 rktvtgadii sameemelqs fvdalsgnla qfrtkkdair akkhggagda asagdadpds
      121 agagdaasag dadpdsagda davsasdaea vsagdaeavs agdaemkddg evdlgddetn
//
LOCUS       XP_077499272             684 aa            linear   INV 25-NOV-2025
DEFINITION  ATP-dependent RNA helicase abstrakt isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077499272
VERSION     XP_077499272.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643146.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..684
                     /product="ATP-dependent RNA helicase abstrakt isoform X1"
                     /calculated_mol_wt=77047
     Region          <51..192
                     /region_name="DUF5401"
                     /note="Family of unknown function (DUF5401); pfam17380"
                     /db_xref="CDD:375164"
     Region          254..459
                     /region_name="DEADc_DDX41"
                     /note="DEAD-box helicase domain of DEAD box protein 41;
                     cd17951"
                     /db_xref="CDD:350709"
     Site            order(262..263,265..267,270,288..295)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350709"
     Region          470..598
                     /region_name="SF2_C_DEAD"
                     /note="C-terminal helicase domain of the DEAD box
                     helicases; cd18787"
                     /db_xref="CDD:350174"
     Site            order(557,559,584,587..588)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350174"
     CDS             1..684
                     /gene="abs"
                     /coded_by="XM_077643146.1:86..2140"
                     /db_xref="GeneID:144110293"
ORIGIN      
        1 mdgansekse ndtenghaqp tarkrsrree sksssdsdsd yapyvplker rkqqlsrlgr
       61 lhailleekr dksstatded ddqrdeygrk snislldqht elkkkaeark esalekqlke
      121 eekilesvae rralmgvael akgiqydqpi qtgwhpptyi anmtleqqee mrqkyrilve
      181 gegipapitt fkvpsgstwp wsgctcaaaw rpqgrqrdrv llpfprpsce etsgppcrgw
      241 yhqchremkf prpilsslrr kgitmptpiq mqglpavlsg rdmigiaftg sgktlvfvlp
      301 llmfcleqek rlpfiqnegp yglivcpsre lakqtfeivs ffvrgledag ypslrgclci
      361 ggtsvreqld vvrrgvhvmv atpgrlmdml dkkmvnldmc rylcldeadr midmgfeedv
      421 rtifsffkgq rqtllfsatm pkkiqnfars alvkpitvnv gragaasldv vqeveyvkqe
      481 akivhlletl qktappvlif aekkqdvdai heylllkgve avaihggkdq eersravdaf
      541 rraekdvlva tdvaskgldf eniqhvinyd mpedienyvh rigrtgrsgr vgiattfink
      601 scdesvlldm khllleakqk vppfllalqs enekylelge ergcsycggl ghritdcpkl
      661 eamqskqasn igrrdylann sadw
//
LOCUS       XP_077499277             630 aa            linear   INV 25-NOV-2025
DEFINITION  ATP-dependent RNA helicase abstrakt isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077499277
VERSION     XP_077499277.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643151.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..630
                     /product="ATP-dependent RNA helicase abstrakt isoform X2"
                     /calculated_mol_wt=70912
     Region          <51..198
                     /region_name="DUF5401"
                     /note="Family of unknown function (DUF5401); pfam17380"
                     /db_xref="CDD:375164"
     Region          200..405
                     /region_name="DEADc_DDX41"
                     /note="DEAD-box helicase domain of DEAD box protein 41;
                     cd17951"
                     /db_xref="CDD:350709"
     Site            order(208..209,211..213,216,234..241)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350709"
     Region          416..544
                     /region_name="SF2_C_DEAD"
                     /note="C-terminal helicase domain of the DEAD box
                     helicases; cd18787"
                     /db_xref="CDD:350174"
     Site            order(503,505,530,533..534)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350174"
     CDS             1..630
                     /gene="abs"
                     /coded_by="XM_077643151.1:86..1978"
                     /db_xref="GeneID:144110293"
ORIGIN      
        1 mdgansekse ndtenghaqp tarkrsrree sksssdsdsd yapyvplker rkqqlsrlgr
       61 lhailleekr dksstatded ddqrdeygrk snislldqht elkkkaeark esalekqlke
      121 eekilesvae rralmgvael akgiqydqpi qtgwhpptyi anmtleqqee mrqkyrilve
      181 gegipapitt fkemkfprpi lsslrrkgit mptpiqmqgl pavlsgrdmi giaftgsgkt
      241 lvfvlpllmf cleqekrlpf iqnegpygli vcpsrelakq tfeivsffvr gledagypsl
      301 rgclciggts vreqldvvrr gvhvmvatpg rlmdmldkkm vnldmcrylc ldeadrmidm
      361 gfeedvrtif sffkgqrqtl lfsatmpkki qnfarsalvk pitvnvgrag aasldvvqev
      421 eyvkqeakiv hlletlqkta ppvlifaekk qdvdaiheyl llkgveavai hggkdqeers
      481 ravdafrrae kdvlvatdva skgldfeniq hvinydmped ienyvhrigr tgrsgrvgia
      541 ttfinkscde svlldmkhll leakqkvppf llalqsenek ylelgeergc sycgglghri
      601 tdcpkleamq skqasnigrr dylannsadw
//
LOCUS       XP_077499285             107 aa            linear   INV 25-NOV-2025
DEFINITION  HIG1 domain family member 2A [Amblyomma americanum].
ACCESSION   XP_077499285
VERSION     XP_077499285.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643159.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..107
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..107
                     /product="HIG1 domain family member 2A"
                     /calculated_mol_wt=11722
     Region          44..94
                     /region_name="HIG_1_N"
                     /note="Hypoxia induced protein conserved region;
                     pfam04588"
                     /db_xref="CDD:461361"
     CDS             1..107
                     /gene="LOC144110307"
                     /coded_by="XM_077643159.1:62..385"
                     /db_xref="GeneID:144110307"
ORIGIN      
        1 madhpkhsnd delewiaiek sfregdavek merstriknk lasnpfvplg llatvtalgf
       61 glrsmfkgqs kqsqmmmrtr vvcqgltfva ilvgivmgar gaqqksq
//
LOCUS       XP_077499295             107 aa            linear   INV 25-NOV-2025
DEFINITION  HIG1 domain family member 2A [Amblyomma americanum].
ACCESSION   XP_077499295
VERSION     XP_077499295.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643169.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..107
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..107
                     /product="HIG1 domain family member 2A"
                     /calculated_mol_wt=11722
     Region          44..94
                     /region_name="HIG_1_N"
                     /note="Hypoxia induced protein conserved region;
                     pfam04588"
                     /db_xref="CDD:461361"
     CDS             1..107
                     /gene="LOC144110307"
                     /coded_by="XM_077643169.1:62..385"
                     /db_xref="GeneID:144110307"
ORIGIN      
        1 madhpkhsnd delewiaiek sfregdavek merstriknk lasnpfvplg llatvtalgf
       61 glrsmfkgqs kqsqmmmrtr vvcqgltfva ilvgivmgar gaqqksq
//
LOCUS       XP_077499305             225 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144110322 [Amblyomma americanum].
ACCESSION   XP_077499305
VERSION     XP_077499305.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643179.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..225
                     /product="uncharacterized protein LOC144110322"
                     /calculated_mol_wt=24278
     CDS             1..225
                     /gene="LOC144110322"
                     /coded_by="XM_077643179.1:40..717"
                     /db_xref="GeneID:144110322"
ORIGIN      
        1 mrqfcacvai tgcadngppp qlfkqscswn kallilcivt hasvcysgde arsnpckgll
       61 vgdmafgsri refdlsenss wvdyveriel scaanklttd ddkravllsc cgpetyslia
      121 tlvkpsrppn vgyqvivdav kkhinpkpse lysstlrrtp rpgnlqpksc wagssgppyp
      181 afarmrvtqa afgsrhhevs slvtpctrgt sgqgpsgclh acrdr
//
LOCUS       XP_077499313            1436 aa            linear   INV 25-NOV-2025
DEFINITION  slit guidance ligand [Amblyomma americanum].
ACCESSION   XP_077499313
VERSION     XP_077499313.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643187.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1436
                     /product="slit guidance ligand"
                     /calculated_mol_wt=160314
     Region          <21..>208
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          54..76
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          77..100
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          101..124
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          125..148
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          149..172
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          173..196
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          178..>255
                     /region_name="PCC"
                     /note="polycystin cation channel protein; TIGR00864"
                     /db_xref="CDD:188093"
     Region          197..289
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          269..300
                     /region_name="LRRNT"
                     /note="Leucine rich repeat N-terminal domain; smart00013"
                     /db_xref="CDD:214470"
     Region          290..321
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <299..>629
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          322..345
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          346..366
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          370..384
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          394..415
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          418..521
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          426..475
                     /region_name="LRRCT"
                     /note="Leucine rich repeat C-terminal domain; smart00082"
                     /db_xref="CDD:214507"
     Region          503..>849
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          522..547
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          548..571
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          572..595
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          596..617
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          620..643
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          644..732
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          733..790
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          746..766
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          791..814
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          815..836
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          820..>895
                     /region_name="PCC"
                     /note="polycystin cation channel protein; TIGR00864"
                     /db_xref="CDD:188093"
     Region          910..941
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     Region          946..982
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          984..1020
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(984,987,1001)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          1023..1059
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          1062..1098
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(1062,1065,1079)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          1170..1303
                     /region_name="LamG"
                     /note="Laminin G domain; smart00282"
                     /db_xref="CDD:214598"
     Region          1368..1436
                     /region_name="CT"
                     /note="C-terminal cystine knot-like domain (CTCK);
                     smart00041"
                     /db_xref="CDD:214482"
     CDS             1..1436
                     /gene="sli"
                     /coded_by="XM_077643187.1:78..4388"
                     /db_xref="GeneID:144110332"
ORIGIN      
        1 maaalllclf fassaaeepq lsrclepcrc tgltldcshr glravphplp pdarrldleg
       61 nnisvigkdd fkgmnklril hlmeneihtv hrsafadlts merlrlnnnk ltslpdslfl
      121 tmanlqrldl shnrlaalge kllrgstllk nlqvdnneis ciddaalrgl rnmeiltlnr
      181 nnltalgkdl fenmkklrvv rlsenplscd cqlswlaswl rrhprlglft rchapaalkg
      241 kavaelhedd mvcemgsgrp agcgrtttcp fpctcldgvv dcrekgltal ppqipestte
      301 lrleqnqite ipsqafshlk wirridlstn liskvapdaf sglksltslv lygnrisdlp
      361 hgifhgltsl qllllnanri scirkdtfrd lrnlnllsly dnsiqslang sfaplnniqt
      421 lhlarnpfic dcnlrwlaly lhqhpvetsg arcegpkrmh rrrlsilgda kfkckgseeh
      481 rtrlagdcvv drdcpeqcvc egtivdcsrk glreiptdlp sfttelrlnd nhivkvrnsg
      541 ifkklpnlsk ldlrnnaise iednaflgae qmadlllten klkqvrakmf sglirlktlm
      601 lrtnqltfig ndtfadldsv rllslydnki rciahgsfer msslatlnll gnplhcschl
      661 rwlsewlrrs dtvtgsprch spahlkdlpl qdmepkqlvc seedlhegcg sddlcprgct
      721 cvghavhcsr qrlrqlpkhl qlstaelyld vndlteipke lnllkdvtri dlsnnqisil
      781 pnnifsnlsk lstlilsynk lqciqvdsfs glkslrilsl hgndvsmipe gtfrdlqsit
      841 hialganply cdcnlrwlsd wikkdyiepg iarcsepnsm kdklvltaps nyfvcagkpq
      901 pdvlakcdac ytfpcqngat cravplrdya ciclpgyhgr nceylvdacy gnpcenhgtc
      961 kvleagrfsc hcpmgfqgdr cetniddcyk nkcennatci dsigsyscvc spgytgnyce
     1021 kkinfcskef npckngatch dhlthytcac vlgfegsncs vnvddcaeqp clngatcvdg
     1081 vnsyhcvcpr gfggahcela pmvamlyprt spcqqhdckh gvcfqpggad yvckcspgfs
     1141 grrcellssv sfredsflef eplqtrpran itlklatrre fgviaytaeg qhlavelfkg
     1201 rvrvsydagn ypvstmfsfe tlpdgeyhsl vlvvdkknvt mtvdggpars vvnegpreyl
     1261 elrsplyvgg lptdvasdal rkwhlrnass flgcmkelyv ndkpmdlmna rkqqrvspgc
     1321 teyddpkpck nhlckrgkci pvdrynyecs crsgwsgpfc dqaptcqkeq yrdhieedgc
     1381 rsqkkvkmae cigscgdhcc rahktkrrsv kmvcplgrty tkhvevvrkc gcsrkc
//
LOCUS       XP_077499342             415 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase KCMF1-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077499342
VERSION     XP_077499342.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643216.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..415
                     /product="E3 ubiquitin-protein ligase KCMF1-like isoform
                     X1"
                     /calculated_mol_wt=44840
     Region          7..55
                     /region_name="ZZ_PCMF_like"
                     /note="Zinc finger, ZZ type. Zinc finger present in
                     potassium channel modulatory factor (PCMF) 1 and related
                     proteins. The ZZ motif coordinates two zinc ions and most
                     likely participates in ligand binding or molecular
                     scaffolding. Human potassium channel...; cd02338"
                     /db_xref="CDD:239078"
     Site            order(9,12,24,27,33,36,46,50)
                     /site_type="other"
                     /note="Zinc-binding sites [ion binding]"
                     /db_xref="CDD:239078"
     Site            order(9,12,33,36)
                     /site_type="other"
                     /note="zinc cluster 1 [ion binding]"
                     /db_xref="CDD:239078"
     Site            order(10,21,23,29,31)
                     /site_type="active"
                     /note="putative charged binding surface [active]"
                     /db_xref="CDD:239078"
     Site            order(22,37,52,55)
                     /site_type="active"
                     /note="putative hydrophobic binding surface [active]"
                     /db_xref="CDD:239078"
     Site            order(24,27,46,50)
                     /site_type="other"
                     /note="zinc cluster 2 [ion binding]"
                     /db_xref="CDD:239078"
     Region          76..137
                     /region_name="zf-Di19"
                     /note="Drought induced 19 protein (Di19), zinc-binding;
                     pfam05605"
                     /db_xref="CDD:428539"
     CDS             1..415
                     /gene="LOC144110360"
                     /coded_by="XM_077643216.1:202..1449"
                     /db_xref="GeneID:144110360"
ORIGIN      
        1 msrhegvscd scmkanfrgk rykclicydy dlcgtcyeag atntrhtpdh amqciltrsd
       61 fdlyyggeav sveqpqsftc picgkmgfte ltlqehasae htdassevvc pvcaalpgge
      121 pnhvtedfat hlslehranr dldepsgsrh vrriphpagr gmssararrs qmqfsstggl
      181 salspsnrds mdpiaggasl haellsqlss gvrrtatlaa psttsqiqql qmqlqlerqq
      241 aqaarqqler lprrqahlas paaaaatlhc pqaattpala psteaptpta hfllsrcvep
      301 slleseqqal elertdrslf aqelllttla erlhwppgga pwpqgtpats vaatsvaatt
      361 ppralgepek ppaersssqq qpdrglsreg grlspsrrkv vrvldernkl kepph
//
LOCUS       XP_077499348             408 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase KCMF1-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077499348
VERSION     XP_077499348.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643222.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..408
                     /product="E3 ubiquitin-protein ligase KCMF1-like isoform
                     X2"
                     /calculated_mol_wt=44247
     Region          7..55
                     /region_name="ZZ_PCMF_like"
                     /note="Zinc finger, ZZ type. Zinc finger present in
                     potassium channel modulatory factor (PCMF) 1 and related
                     proteins. The ZZ motif coordinates two zinc ions and most
                     likely participates in ligand binding or molecular
                     scaffolding. Human potassium channel...; cd02338"
                     /db_xref="CDD:239078"
     Site            order(9,12,24,27,33,36,46,50)
                     /site_type="other"
                     /note="Zinc-binding sites [ion binding]"
                     /db_xref="CDD:239078"
     Site            order(9,12,33,36)
                     /site_type="other"
                     /note="zinc cluster 1 [ion binding]"
                     /db_xref="CDD:239078"
     Site            order(10,21,23,29,31)
                     /site_type="active"
                     /note="putative charged binding surface [active]"
                     /db_xref="CDD:239078"
     Site            order(22,37,52,55)
                     /site_type="active"
                     /note="putative hydrophobic binding surface [active]"
                     /db_xref="CDD:239078"
     Site            order(24,27,46,50)
                     /site_type="other"
                     /note="zinc cluster 2 [ion binding]"
                     /db_xref="CDD:239078"
     Region          76..137
                     /region_name="zf-Di19"
                     /note="Drought induced 19 protein (Di19), zinc-binding;
                     pfam05605"
                     /db_xref="CDD:428539"
     CDS             1..408
                     /gene="LOC144110360"
                     /coded_by="XM_077643222.1:202..1428"
                     /db_xref="GeneID:144110360"
ORIGIN      
        1 msrhegvscd scmkanfrgk rykclicydy dlcgtcyeag atntrhtpdh amqciltrsd
       61 fdlyyggeav sveqpqsftc picgkmgfte ltlqehasae htdassevvc pvcaalpgge
      121 pnhvtedfat hlslehranr dldepsgsrh vrriphpagr gmssararrs qmqfsstggl
      181 salspsnrds mdpiaellsq lssgvrrtat laapsttsqi qqlqmqlqle rqqaqaarqq
      241 lerlprrqah laspaaaaat lhcpqaattp alapsteapt ptahfllsrc vepslleseq
      301 qalelertdr slfaqelllt tlaerlhwpp ggapwpqgtp atsvaatsva attppralge
      361 pekppaerss sqqqpdrgls reggrlspsr rkvvrvlder nklkepph
//
LOCUS       XP_077499355             396 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase KCMF1-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077499355
VERSION     XP_077499355.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643229.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..396
                     /product="E3 ubiquitin-protein ligase KCMF1-like isoform
                     X3"
                     /calculated_mol_wt=42954
     Region          2..43
                     /region_name="ZZ_PCMF_like"
                     /note="Zinc finger, ZZ type. Zinc finger present in
                     potassium channel modulatory factor (PCMF) 1 and related
                     proteins. The ZZ motif coordinates two zinc ions and most
                     likely participates in ligand binding or molecular
                     scaffolding. Human potassium channel...; cd02338"
                     /db_xref="CDD:239078"
     Site            order(9,11,17,19)
                     /site_type="active"
                     /note="putative charged binding surface [active]"
                     /db_xref="CDD:239078"
     Site            order(10,25,40,43)
                     /site_type="active"
                     /note="putative hydrophobic binding surface [active]"
                     /db_xref="CDD:239078"
     Site            order(12,15,34,38)
                     /site_type="other"
                     /note="zinc cluster 2 [ion binding]"
                     /db_xref="CDD:239078"
     Region          64..125
                     /region_name="zf-Di19"
                     /note="Drought induced 19 protein (Di19), zinc-binding;
                     pfam05605"
                     /db_xref="CDD:428539"
     CDS             1..396
                     /gene="LOC144110360"
                     /coded_by="XM_077643229.1:64..1254"
                     /db_xref="GeneID:144110360"
ORIGIN      
        1 mkanfrgkry kclicydydl cgtcyeagat ntrhtpdham qciltrsdfd lyyggeavsv
       61 eqpqsftcpi cgkmgftelt lqehasaeht dassevvcpv caalpggepn hvtedfathl
      121 slehranrdl depsgsrhvr riphpagrgm ssararrsqm qfsstgglsa lspsnrdsmd
      181 piaellsqls sgvrrtatla apsttsqiqq lqmqlqlerq qaqaarqqle rlprrqahla
      241 spaaaaatlh cpqaattpal apsteaptpt ahfllsrcve pslleseqqa lelertdrsl
      301 faqelllttl aerlhwppgg apwpqgtpat svaatsvaat tppralgepe kppaersssq
      361 qqpdrglsre ggrlspsrrk vvrvldernk lkepph
//
LOCUS       XP_077499369             267 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144110388 [Amblyomma americanum].
ACCESSION   XP_077499369
VERSION     XP_077499369.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643243.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..267
                     /product="uncharacterized protein LOC144110388"
                     /calculated_mol_wt=28423
     CDS             1..267
                     /gene="LOC144110388"
                     /coded_by="XM_077643243.1:108..911"
                     /db_xref="GeneID:144110388"
ORIGIN      
        1 mtrvakvifg gsttttgrad gpcrrrrkcr alasgspqsl rrpcgrstwt wslacchpcs
       61 llrpnsrrag vavatngdae aaaacgaayd dpftsanppv kqrgtlldvs plhashvaag
      121 lvhrhgrdsc wqrvgpalcs rnasrargsd asgavlprlg psnslegtlp hpelplppqf
      181 pwpqqghalp ggpislprqe etgpwgtgpg frprsgrlql pgwpyigepv faaianprrv
      241 lmmdngtwyi lvysafpend ecrttna
//
LOCUS       XP_077499383             709 aa            linear   INV 25-NOV-2025
DEFINITION  poly(U)-binding-splicing factor hfp isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077499383
VERSION     XP_077499383.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643257.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..709
                     /product="poly(U)-binding-splicing factor hfp isoform X1"
                     /calculated_mol_wt=74940
     Region          117..709
                     /region_name="half-pint"
                     /note="poly-U binding splicing factor, half-pint family;
                     TIGR01645"
                     /db_xref="CDD:130706"
     CDS             1..709
                     /gene="hfp"
                     /coded_by="XM_077643257.1:7..2136"
                     /db_xref="GeneID:144110401"
ORIGIN      
        1 mqtrsprpcs ciglsaarcl errglssfal padrekmssq apvendvslq ngdkdchpek
       61 geteaehlts eaenssdgpp akkaalqdvp asssggsggp ggttstgtke egttqqeqmg
      121 pvlagpgalk aappligfgl mklspeqlem vtrakkfame qsiksvlmkq tiahqqqqqk
      181 tlqrhqalvl mcrwcrvyvg sisfelkedt ikqafrpfgp iksinmswdp vtqkhkgfaf
      241 veyelpeaaq laleqmngvl iggrnikvgr psnmpqaapi ldqimeeakt ynriyiasvh
      301 qdltesdiqs vfeafgkirs cklipsstpg khkeygfiey etnvganeai asmnlfdlgg
      361 qylrvgrait ppnaltppsa qsvmptaaav aaaaatakiq amdavannal alglsspgav
      421 lsssaaalgl sspgvigsss aaavipppgl tiptlvppgl vgppgvlpqp vlaanapgii
      481 tgvtpmppta aalipppsiv ttlplaaakp atdllgaaal atttaaveta lsqatitqaa
      541 lnqvvlnqaa lkqpvvsqap ateapkeedd lakkiaeele pqtlqqqenm mikgsnarhm
      601 lmrklmrrte scvvvlrnmv gvedlddele sevtdecgrf gsvkrviiyq erqsedenae
      661 ivvkifvefs qaqesasard alngrffggr ivkaelydqt lyeandlsg
//
LOCUS       XP_077499392             707 aa            linear   INV 25-NOV-2025
DEFINITION  poly(U)-binding-splicing factor hfp isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077499392
VERSION     XP_077499392.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643266.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..707
                     /product="poly(U)-binding-splicing factor hfp isoform X2"
                     /calculated_mol_wt=74684
     Region          117..707
                     /region_name="half-pint"
                     /note="poly-U binding splicing factor, half-pint family;
                     TIGR01645"
                     /db_xref="CDD:130706"
     CDS             1..707
                     /gene="hfp"
                     /coded_by="XM_077643266.1:7..2130"
                     /db_xref="GeneID:144110401"
ORIGIN      
        1 mqtrsprpcs ciglsaarcl errglssfal padrekmssq apvendvslq ngdkdchpek
       61 geteaehlts eaenssdgpp akkaalqdvp asssggsggp ggttstgtke egttqqeqmg
      121 pvlagpgalk aappligfgl mklspeqlem vtrakkfame qsiksvlmkq tiahqqqktl
      181 qrhqalvlmc rwcrvyvgsi sfelkedtik qafrpfgpik sinmswdpvt qkhkgfafve
      241 yelpeaaqla leqmngvlig grnikvgrps nmpqaapild qimeeaktyn riyiasvhqd
      301 ltesdiqsvf eafgkirsck lipsstpgkh keygfieyet nvganeaias mnlfdlggqy
      361 lrvgraitpp naltppsaqs vmptaaavaa aaatakiqam davannalal glsspgavls
      421 ssaaalglss pgvigsssaa avipppglti ptlvppglvg ppgvlpqpvl aanapgiitg
      481 vtpmpptaaa lipppsivtt lplaaakpat dllgaaalat ttaavetals qatitqaaln
      541 qvvlnqaalk qpvvsqapat eapkeeddla kkiaeelepq tlqqqenmmi kgsnarhmlm
      601 rklmrrtesc vvvlrnmvgv edlddelese vtdecgrfgs vkrviiyqer qsedenaeiv
      661 vkifvefsqa qesasardal ngrffggriv kaelydqtly eandlsg
//
LOCUS       XP_077499401             706 aa            linear   INV 25-NOV-2025
DEFINITION  poly(U)-binding-splicing factor hfp isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077499401
VERSION     XP_077499401.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643275.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..706
                     /product="poly(U)-binding-splicing factor hfp isoform X3"
                     /calculated_mol_wt=74494
     Region          117..706
                     /region_name="half-pint"
                     /note="poly-U binding splicing factor, half-pint family;
                     TIGR01645"
                     /db_xref="CDD:130706"
     CDS             1..706
                     /gene="hfp"
                     /coded_by="XM_077643275.1:6..2126"
                     /db_xref="GeneID:144110401"
ORIGIN      
        1 mqtrsprpcs ciglsaarcl errglssfal padrekmssq apvendvslq ngdkdchpek
       61 geteaehlts eaenssdgpp akkaalqdvp asssggsggp ggttstgtke egttqqeqmg
      121 pvlagpgalk aappligfgl mklspeqlem vtrakkfame qsiksvlmkq tiahqqqqqk
      181 tlqrhqalvl mcrvyvgsis felkedtikq afrpfgpiks inmswdpvtq khkgfafvey
      241 elpeaaqlal eqmngvligg rnikvgrpsn mpqaapildq imeeaktynr iyiasvhqdl
      301 tesdiqsvfe afgkirsckl ipsstpgkhk eygfieyetn vganeaiasm nlfdlggqyl
      361 rvgraitppn altppsaqsv mptaaavaaa aatakiqamd avannalalg lsspgavlss
      421 saaalglssp gvigsssaaa vipppgltip tlvppglvgp pgvlpqpvla anapgiitgv
      481 tpmpptaaal ipppsivttl plaaakpatd llgaaalatt taavetalsq atitqaalnq
      541 vvlnqaalkq pvvsqapate apkeeddlak kiaeelepqt lqqqenmmik gsnarhmlmr
      601 klmrrtescv vvlrnmvgve dlddelesev tdecgrfgsv krviiyqerq sedenaeivv
      661 kifvefsqaq esasardaln grffggrivk aelydqtlye andlsg
//
LOCUS       XP_077499412             704 aa            linear   INV 25-NOV-2025
DEFINITION  poly(U)-binding-splicing factor hfp isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077499412
VERSION     XP_077499412.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643286.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..704
                     /product="poly(U)-binding-splicing factor hfp isoform X4"
                     /calculated_mol_wt=74238
     Region          117..704
                     /region_name="half-pint"
                     /note="poly-U binding splicing factor, half-pint family;
                     TIGR01645"
                     /db_xref="CDD:130706"
     CDS             1..704
                     /gene="hfp"
                     /coded_by="XM_077643286.1:7..2121"
                     /db_xref="GeneID:144110401"
ORIGIN      
        1 mqtrsprpcs ciglsaarcl errglssfal padrekmssq apvendvslq ngdkdchpek
       61 geteaehlts eaenssdgpp akkaalqdvp asssggsggp ggttstgtke egttqqeqmg
      121 pvlagpgalk aappligfgl mklspeqlem vtrakkfame qsiksvlmkq tiahqqqktl
      181 qrhqalvlmc rvyvgsisfe lkedtikqaf rpfgpiksin mswdpvtqkh kgfafveyel
      241 peaaqlaleq mngvliggrn ikvgrpsnmp qaapildqim eeaktynriy iasvhqdlte
      301 sdiqsvfeaf gkirscklip sstpgkhkey gfieyetnvg aneaiasmnl fdlggqylrv
      361 graitppnal tppsaqsvmp taaavaaaaa takiqamdav annalalgls spgavlsssa
      421 aalglsspgv igsssaaavi pppgltiptl vppglvgppg vlpqpvlaan apgiitgvtp
      481 mpptaaalip ppsivttlpl aaakpatdll gaaalattta avetalsqat itqaalnqvv
      541 lnqaalkqpv vsqapateap keeddlakki aeelepqtlq qqenmmikgs narhmlmrkl
      601 mrrtescvvv lrnmvgvedl ddelesevtd ecgrfgsvkr viiyqerqse denaeivvki
      661 fvefsqaqes asardalngr ffggrivkae lydqtlyean dlsg
//
LOCUS       XP_077499419             703 aa            linear   INV 25-NOV-2025
DEFINITION  poly(U)-binding-splicing factor hfp isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077499419
VERSION     XP_077499419.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643293.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..703
                     /product="poly(U)-binding-splicing factor hfp isoform X5"
                     /calculated_mol_wt=74321
     Region          111..703
                     /region_name="half-pint"
                     /note="poly-U binding splicing factor, half-pint family;
                     TIGR01645"
                     /db_xref="CDD:130706"
     CDS             1..703
                     /gene="hfp"
                     /coded_by="XM_077643293.1:140..2251"
                     /db_xref="GeneID:144110401"
ORIGIN      
        1 mffsfcvfft pfsqeracga shgvdtmrtv anvtkrmtge lafpapffga dpastasmas
       61 imrgcysrpk ttmfidgage gdvpasssgg sggpggttst gtkeegttqq eqmgpvlagp
      121 galkaappli gfglmklspe qlemvtrakk fameqsiksv lmkqtiahqq qqqktlqrhq
      181 alvlmcrwcr vyvgsisfel kedtikqafr pfgpiksinm swdpvtqkhk gfafveyelp
      241 eaaqlaleqm ngvliggrni kvgrpsnmpq aapildqime eaktynriyi asvhqdltes
      301 diqsvfeafg kirscklips stpgkhkeyg fieyetnvga neaiasmnlf dlggqylrvg
      361 raitppnalt ppsaqsvmpt aaavaaaaat akiqamdava nnalalglss pgavlsssaa
      421 alglsspgvi gsssaaavip ppgltiptlv ppglvgppgv lpqpvlaana pgiitgvtpm
      481 pptaaalipp psivttlpla aakpatdllg aaalatttaa vetalsqati tqaalnqvvl
      541 nqaalkqpvv sqapateapk eeddlakkia eelepqtlqq qenmmikgsn arhmlmrklm
      601 rrtescvvvl rnmvgvedld delesevtde cgrfgsvkrv iiyqerqsed enaeivvkif
      661 vefsqaqesa sardalngrf fggrivkael ydqtlyeand lsg
//
LOCUS       XP_077499428             693 aa            linear   INV 25-NOV-2025
DEFINITION  poly(U)-binding-splicing factor hfp isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077499428
VERSION     XP_077499428.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643302.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..693
                     /product="poly(U)-binding-splicing factor hfp isoform X6"
                     /calculated_mol_wt=73570
     Region          117..693
                     /region_name="half-pint"
                     /note="poly-U binding splicing factor, half-pint family;
                     TIGR01645"
                     /db_xref="CDD:130706"
     CDS             1..693
                     /gene="hfp"
                     /coded_by="XM_077643302.1:6..2087"
                     /db_xref="GeneID:144110401"
ORIGIN      
        1 mqtrsprpcs ciglsaarcl errglssfal padrekmssq apvendvslq ngdkdchpek
       61 geteaehlts eaenssdgpp akkaalqdvp asssggsggp ggttstgtke egttqqeqmg
      121 pvlagpgalk aappligfgl mklspeqlem vtrakkfame qsiksvlmkq tiahqqqqqk
      181 tlqrhqalvl mcrwcrvyvg sisfelkedt ikqafrpfgp iksinmswdp vtqkhkgfaf
      241 veyelpeaaq laleqmngvl iggrnikvgr psnmpqaapi ldqimeeakt ynriyiasvh
      301 qdltesdiqs vfeafgkirs cklipsstpg khkeygfiey etnvganeai asmnlfdlgg
      361 qylrvgrait ppnaltppsa qsvmptaaav aaaaatakiq amdavannal alglsspgvi
      421 gsssaaavip ppgltiptlv ppglvgppgv lpqpvlaana pgiitgvtpm pptaaalipp
      481 psivttlpla aakpatdllg aaalatttaa vetalsqati tqaalnqvvl nqaalkqpvv
      541 sqapateapk eeddlakkia eelepqtlqq qenmmikgsn arhmlmrklm rrtescvvvl
      601 rnmvgvedld delesevtde cgrfgsvkrv iiyqerqsed enaeivvkif vefsqaqesa
      661 sardalngrf fggrivkael ydqtlyeand lsg
//
LOCUS       XP_077499436             690 aa            linear   INV 25-NOV-2025
DEFINITION  poly(U)-binding-splicing factor hfp isoform X7 [Amblyomma
            americanum].
ACCESSION   XP_077499436
VERSION     XP_077499436.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643310.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..690
                     /product="poly(U)-binding-splicing factor hfp isoform X7"
                     /calculated_mol_wt=73125
     Region          117..690
                     /region_name="half-pint"
                     /note="poly-U binding splicing factor, half-pint family;
                     TIGR01645"
                     /db_xref="CDD:130706"
     CDS             1..690
                     /gene="hfp"
                     /coded_by="XM_077643310.1:5..2077"
                     /db_xref="GeneID:144110401"
ORIGIN      
        1 mqtrsprpcs ciglsaarcl errglssfal padrekmssq apvendvslq ngdkdchpek
       61 geteaehlts eaenssdgpp akkaalqdvp asssggsggp ggttstgtke egttqqeqmg
      121 pvlagpgalk aappligfgl mklspeqlem vtrakkfame qsiksvlmkq tiahqqqqqk
      181 tlqrhqalvl mcrvyvgsis felkedtikq afrpfgpiks inmswdpvtq khkgfafvey
      241 elpeaaqlal eqmngvligg rnikvgrpsn mpqaapildq imeeaktynr iyiasvhqdl
      301 tesdiqsvfe afgkirsckl ipsstpgkhk eygfieyetn vganeaiasm nlfdlggqyl
      361 rvgraitppn altppsaqsv mptaaavaaa aatakiqamd avannalalg lsspgvigss
      421 saaavipppg ltiptlvppg lvgppgvlpq pvlaanapgi itgvtpmppt aaalipppsi
      481 vttlplaaak patdllgaaa latttaavet alsqatitqa alnqvvlnqa alkqpvvsqa
      541 pateapkeed dlakkiaeel epqtlqqqen mmikgsnarh mlmrklmrrt escvvvlrnm
      601 vgvedlddel esevtdecgr fgsvkrviiy qerqsedena eivvkifvef sqaqesasar
      661 dalngrffgg rivkaelydq tlyeandlsg
//
LOCUS       XP_077499447             688 aa            linear   INV 25-NOV-2025
DEFINITION  poly(U)-binding-splicing factor hfp isoform X8 [Amblyomma
            americanum].
ACCESSION   XP_077499447
VERSION     XP_077499447.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643321.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..688
                     /product="poly(U)-binding-splicing factor hfp isoform X8"
                     /calculated_mol_wt=72869
     Region          117..688
                     /region_name="half-pint"
                     /note="poly-U binding splicing factor, half-pint family;
                     TIGR01645"
                     /db_xref="CDD:130706"
     CDS             1..688
                     /gene="hfp"
                     /coded_by="XM_077643321.1:6..2072"
                     /db_xref="GeneID:144110401"
ORIGIN      
        1 mqtrsprpcs ciglsaarcl errglssfal padrekmssq apvendvslq ngdkdchpek
       61 geteaehlts eaenssdgpp akkaalqdvp asssggsggp ggttstgtke egttqqeqmg
      121 pvlagpgalk aappligfgl mklspeqlem vtrakkfame qsiksvlmkq tiahqqqktl
      181 qrhqalvlmc rvyvgsisfe lkedtikqaf rpfgpiksin mswdpvtqkh kgfafveyel
      241 peaaqlaleq mngvliggrn ikvgrpsnmp qaapildqim eeaktynriy iasvhqdlte
      301 sdiqsvfeaf gkirscklip sstpgkhkey gfieyetnvg aneaiasmnl fdlggqylrv
      361 graitppnal tppsaqsvmp taaavaaaaa takiqamdav annalalgls spgvigsssa
      421 aavipppglt iptlvppglv gppgvlpqpv laanapgiit gvtpmpptaa alipppsivt
      481 tlplaaakpa tdllgaaala tttaavetal sqatitqaal nqvvlnqaal kqpvvsqapa
      541 teapkeeddl akkiaeelep qtlqqqenmm ikgsnarhml mrklmrrtes cvvvlrnmvg
      601 vedlddeles evtdecgrfg svkrviiyqe rqsedenaei vvkifvefsq aqesasarda
      661 lngrffggri vkaelydqtl yeandlsg
//
LOCUS       XP_077499454             677 aa            linear   INV 25-NOV-2025
DEFINITION  poly(U)-binding-splicing factor hfp isoform X9 [Amblyomma
            americanum].
ACCESSION   XP_077499454
VERSION     XP_077499454.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643328.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..677
                     /product="poly(U)-binding-splicing factor hfp isoform X9"
                     /calculated_mol_wt=71419
     Region          85..677
                     /region_name="half-pint"
                     /note="poly-U binding splicing factor, half-pint family;
                     TIGR01645"
                     /db_xref="CDD:130706"
     CDS             1..677
                     /gene="hfp"
                     /coded_by="XM_077643328.1:462..2495"
                     /db_xref="GeneID:144110401"
ORIGIN      
        1 mrtvanvtkr mtgelafpap ffgadpasta smasimrgcy srpkttmfid gagegdvpas
       61 ssggsggpgg ttstgtkeeg ttqqeqmgpv lagpgalkaa ppligfglmk lspeqlemvt
      121 rakkfameqs iksvlmkqti ahqqqqqktl qrhqalvlmc rwcrvyvgsi sfelkedtik
      181 qafrpfgpik sinmswdpvt qkhkgfafve yelpeaaqla leqmngvlig grnikvgrps
      241 nmpqaapild qimeeaktyn riyiasvhqd ltesdiqsvf eafgkirsck lipsstpgkh
      301 keygfieyet nvganeaias mnlfdlggqy lrvgraitpp naltppsaqs vmptaaavaa
      361 aaatakiqam davannalal glsspgavls ssaaalglss pgvigsssaa avipppglti
      421 ptlvppglvg ppgvlpqpvl aanapgiitg vtpmpptaaa lipppsivtt lplaaakpat
      481 dllgaaalat ttaavetals qatitqaaln qvvlnqaalk qpvvsqapat eapkeeddla
      541 kkiaeelepq tlqqqenmmi kgsnarhmlm rklmrrtesc vvvlrnmvgv edlddelese
      601 vtdecgrfgs vkrviiyqer qsedenaeiv vkifvefsqa qesasardal ngrffggriv
      661 kaelydqtly eandlsg
//
LOCUS       XP_077499460             677 aa            linear   INV 25-NOV-2025
DEFINITION  poly(U)-binding-splicing factor hfp isoform X9 [Amblyomma
            americanum].
ACCESSION   XP_077499460
VERSION     XP_077499460.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643334.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..677
                     /product="poly(U)-binding-splicing factor hfp isoform X9"
                     /calculated_mol_wt=71419
     Region          85..677
                     /region_name="half-pint"
                     /note="poly-U binding splicing factor, half-pint family;
                     TIGR01645"
                     /db_xref="CDD:130706"
     CDS             1..677
                     /gene="hfp"
                     /coded_by="XM_077643334.1:337..2370"
                     /db_xref="GeneID:144110401"
ORIGIN      
        1 mrtvanvtkr mtgelafpap ffgadpasta smasimrgcy srpkttmfid gagegdvpas
       61 ssggsggpgg ttstgtkeeg ttqqeqmgpv lagpgalkaa ppligfglmk lspeqlemvt
      121 rakkfameqs iksvlmkqti ahqqqqqktl qrhqalvlmc rwcrvyvgsi sfelkedtik
      181 qafrpfgpik sinmswdpvt qkhkgfafve yelpeaaqla leqmngvlig grnikvgrps
      241 nmpqaapild qimeeaktyn riyiasvhqd ltesdiqsvf eafgkirsck lipsstpgkh
      301 keygfieyet nvganeaias mnlfdlggqy lrvgraitpp naltppsaqs vmptaaavaa
      361 aaatakiqam davannalal glsspgavls ssaaalglss pgvigsssaa avipppglti
      421 ptlvppglvg ppgvlpqpvl aanapgiitg vtpmpptaaa lipppsivtt lplaaakpat
      481 dllgaaalat ttaavetals qatitqaaln qvvlnqaalk qpvvsqapat eapkeeddla
      541 kkiaeelepq tlqqqenmmi kgsnarhmlm rklmrrtesc vvvlrnmvgv edlddelese
      601 vtdecgrfgs vkrviiyqer qsedenaeiv vkifvefsqa qesasardal ngrffggriv
      661 kaelydqtly eandlsg
//
LOCUS       XP_077499465             677 aa            linear   INV 25-NOV-2025
DEFINITION  poly(U)-binding-splicing factor hfp isoform X9 [Amblyomma
            americanum].
ACCESSION   XP_077499465
VERSION     XP_077499465.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643339.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..677
                     /product="poly(U)-binding-splicing factor hfp isoform X9"
                     /calculated_mol_wt=71419
     Region          85..677
                     /region_name="half-pint"
                     /note="poly-U binding splicing factor, half-pint family;
                     TIGR01645"
                     /db_xref="CDD:130706"
     CDS             1..677
                     /gene="hfp"
                     /coded_by="XM_077643339.1:397..2430"
                     /db_xref="GeneID:144110401"
ORIGIN      
        1 mrtvanvtkr mtgelafpap ffgadpasta smasimrgcy srpkttmfid gagegdvpas
       61 ssggsggpgg ttstgtkeeg ttqqeqmgpv lagpgalkaa ppligfglmk lspeqlemvt
      121 rakkfameqs iksvlmkqti ahqqqqqktl qrhqalvlmc rwcrvyvgsi sfelkedtik
      181 qafrpfgpik sinmswdpvt qkhkgfafve yelpeaaqla leqmngvlig grnikvgrps
      241 nmpqaapild qimeeaktyn riyiasvhqd ltesdiqsvf eafgkirsck lipsstpgkh
      301 keygfieyet nvganeaias mnlfdlggqy lrvgraitpp naltppsaqs vmptaaavaa
      361 aaatakiqam davannalal glsspgavls ssaaalglss pgvigsssaa avipppglti
      421 ptlvppglvg ppgvlpqpvl aanapgiitg vtpmpptaaa lipppsivtt lplaaakpat
      481 dllgaaalat ttaavetals qatitqaaln qvvlnqaalk qpvvsqapat eapkeeddla
      541 kkiaeelepq tlqqqenmmi kgsnarhmlm rklmrrtesc vvvlrnmvgv edlddelese
      601 vtdecgrfgs vkrviiyqer qsedenaeiv vkifvefsqa qesasardal ngrffggriv
      661 kaelydqtly eandlsg
//
LOCUS       XP_077499473             677 aa            linear   INV 25-NOV-2025
DEFINITION  poly(U)-binding-splicing factor hfp isoform X9 [Amblyomma
            americanum].
ACCESSION   XP_077499473
VERSION     XP_077499473.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643347.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..677
                     /product="poly(U)-binding-splicing factor hfp isoform X9"
                     /calculated_mol_wt=71419
     Region          85..677
                     /region_name="half-pint"
                     /note="poly-U binding splicing factor, half-pint family;
                     TIGR01645"
                     /db_xref="CDD:130706"
     CDS             1..677
                     /gene="hfp"
                     /coded_by="XM_077643347.1:135..2168"
                     /db_xref="GeneID:144110401"
ORIGIN      
        1 mrtvanvtkr mtgelafpap ffgadpasta smasimrgcy srpkttmfid gagegdvpas
       61 ssggsggpgg ttstgtkeeg ttqqeqmgpv lagpgalkaa ppligfglmk lspeqlemvt
      121 rakkfameqs iksvlmkqti ahqqqqqktl qrhqalvlmc rwcrvyvgsi sfelkedtik
      181 qafrpfgpik sinmswdpvt qkhkgfafve yelpeaaqla leqmngvlig grnikvgrps
      241 nmpqaapild qimeeaktyn riyiasvhqd ltesdiqsvf eafgkirsck lipsstpgkh
      301 keygfieyet nvganeaias mnlfdlggqy lrvgraitpp naltppsaqs vmptaaavaa
      361 aaatakiqam davannalal glsspgavls ssaaalglss pgvigsssaa avipppglti
      421 ptlvppglvg ppgvlpqpvl aanapgiitg vtpmpptaaa lipppsivtt lplaaakpat
      481 dllgaaalat ttaavetals qatitqaaln qvvlnqaalk qpvvsqapat eapkeeddla
      541 kkiaeelepq tlqqqenmmi kgsnarhmlm rklmrrtesc vvvlrnmvgv edlddelese
      601 vtdecgrfgs vkrviiyqer qsedenaeiv vkifvefsqa qesasardal ngrffggriv
      661 kaelydqtly eandlsg
//
LOCUS       XP_077499480             591 aa            linear   INV 25-NOV-2025
DEFINITION  poly(U)-binding-splicing factor hfp isoform X10 [Amblyomma
            americanum].
ACCESSION   XP_077499480
VERSION     XP_077499480.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643354.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..591
                     /product="poly(U)-binding-splicing factor hfp isoform X10"
                     /calculated_mol_wt=62755
     Region          2..591
                     /region_name="half-pint"
                     /note="poly-U binding splicing factor, half-pint family;
                     TIGR01645"
                     /db_xref="CDD:130706"
     CDS             1..591
                     /gene="hfp"
                     /coded_by="XM_077643354.1:602..2377"
                     /db_xref="GeneID:144110401"
ORIGIN      
        1 mgpvlagpga lkaappligf glmklspeql emvtrakkfa meqsiksvlm kqtiahqqqq
       61 qktlqrhqal vlmcrwcrvy vgsisfelke dtikqafrpf gpiksinmsw dpvtqkhkgf
      121 afveyelpea aqlaleqmng vliggrnikv grpsnmpqaa pildqimeea ktynriyias
      181 vhqdltesdi qsvfeafgki rscklipsst pgkhkeygfi eyetnvgane aiasmnlfdl
      241 ggqylrvgra itppnaltpp saqsvmptaa avaaaaatak iqamdavann alalglsspg
      301 avlsssaaal glsspgvigs ssaaavippp gltiptlvpp glvgppgvlp qpvlaanapg
      361 iitgvtpmpp taaalippps ivttlplaaa kpatdllgaa alatttaave talsqatitq
      421 aalnqvvlnq aalkqpvvsq apateapkee ddlakkiaee lepqtlqqqe nmmikgsnar
      481 hmlmrklmrr tescvvvlrn mvgvedldde lesevtdecg rfgsvkrvii yqerqseden
      541 aeivvkifve fsqaqesasa rdalngrffg grivkaelyd qtlyeandls g
//
LOCUS       XP_077499488             591 aa            linear   INV 25-NOV-2025
DEFINITION  poly(U)-binding-splicing factor hfp isoform X10 [Amblyomma
            americanum].
ACCESSION   XP_077499488
VERSION     XP_077499488.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643362.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..591
                     /product="poly(U)-binding-splicing factor hfp isoform X10"
                     /calculated_mol_wt=62755
     Region          2..591
                     /region_name="half-pint"
                     /note="poly-U binding splicing factor, half-pint family;
                     TIGR01645"
                     /db_xref="CDD:130706"
     CDS             1..591
                     /gene="hfp"
                     /coded_by="XM_077643362.1:541..2316"
                     /db_xref="GeneID:144110401"
ORIGIN      
        1 mgpvlagpga lkaappligf glmklspeql emvtrakkfa meqsiksvlm kqtiahqqqq
       61 qktlqrhqal vlmcrwcrvy vgsisfelke dtikqafrpf gpiksinmsw dpvtqkhkgf
      121 afveyelpea aqlaleqmng vliggrnikv grpsnmpqaa pildqimeea ktynriyias
      181 vhqdltesdi qsvfeafgki rscklipsst pgkhkeygfi eyetnvgane aiasmnlfdl
      241 ggqylrvgra itppnaltpp saqsvmptaa avaaaaatak iqamdavann alalglsspg
      301 avlsssaaal glsspgvigs ssaaavippp gltiptlvpp glvgppgvlp qpvlaanapg
      361 iitgvtpmpp taaalippps ivttlplaaa kpatdllgaa alatttaave talsqatitq
      421 aalnqvvlnq aalkqpvvsq apateapkee ddlakkiaee lepqtlqqqe nmmikgsnar
      481 hmlmrklmrr tescvvvlrn mvgvedldde lesevtdecg rfgsvkrvii yqerqseden
      541 aeivvkifve fsqaqesasa rdalngrffg grivkaelyd qtlyeandls g
//
LOCUS       XP_077499497             591 aa            linear   INV 25-NOV-2025
DEFINITION  poly(U)-binding-splicing factor hfp isoform X10 [Amblyomma
            americanum].
ACCESSION   XP_077499497
VERSION     XP_077499497.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643371.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..591
                     /product="poly(U)-binding-splicing factor hfp isoform X10"
                     /calculated_mol_wt=62755
     Region          2..591
                     /region_name="half-pint"
                     /note="poly-U binding splicing factor, half-pint family;
                     TIGR01645"
                     /db_xref="CDD:130706"
     CDS             1..591
                     /gene="hfp"
                     /coded_by="XM_077643371.1:233..2008"
                     /db_xref="GeneID:144110401"
ORIGIN      
        1 mgpvlagpga lkaappligf glmklspeql emvtrakkfa meqsiksvlm kqtiahqqqq
       61 qktlqrhqal vlmcrwcrvy vgsisfelke dtikqafrpf gpiksinmsw dpvtqkhkgf
      121 afveyelpea aqlaleqmng vliggrnikv grpsnmpqaa pildqimeea ktynriyias
      181 vhqdltesdi qsvfeafgki rscklipsst pgkhkeygfi eyetnvgane aiasmnlfdl
      241 ggqylrvgra itppnaltpp saqsvmptaa avaaaaatak iqamdavann alalglsspg
      301 avlsssaaal glsspgvigs ssaaavippp gltiptlvpp glvgppgvlp qpvlaanapg
      361 iitgvtpmpp taaalippps ivttlplaaa kpatdllgaa alatttaave talsqatitq
      421 aalnqvvlnq aalkqpvvsq apateapkee ddlakkiaee lepqtlqqqe nmmikgsnar
      481 hmlmrklmrr tescvvvlrn mvgvedldde lesevtdecg rfgsvkrvii yqerqseden
      541 aeivvkifve fsqaqesasa rdalngrffg grivkaelyd qtlyeandls g
//
LOCUS       XP_077499505             591 aa            linear   INV 25-NOV-2025
DEFINITION  poly(U)-binding-splicing factor hfp isoform X10 [Amblyomma
            americanum].
ACCESSION   XP_077499505
VERSION     XP_077499505.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643379.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..591
                     /product="poly(U)-binding-splicing factor hfp isoform X10"
                     /calculated_mol_wt=62755
     Region          2..591
                     /region_name="half-pint"
                     /note="poly-U binding splicing factor, half-pint family;
                     TIGR01645"
                     /db_xref="CDD:130706"
     CDS             1..591
                     /gene="hfp"
                     /coded_by="XM_077643379.1:230..2005"
                     /db_xref="GeneID:144110401"
ORIGIN      
        1 mgpvlagpga lkaappligf glmklspeql emvtrakkfa meqsiksvlm kqtiahqqqq
       61 qktlqrhqal vlmcrwcrvy vgsisfelke dtikqafrpf gpiksinmsw dpvtqkhkgf
      121 afveyelpea aqlaleqmng vliggrnikv grpsnmpqaa pildqimeea ktynriyias
      181 vhqdltesdi qsvfeafgki rscklipsst pgkhkeygfi eyetnvgane aiasmnlfdl
      241 ggqylrvgra itppnaltpp saqsvmptaa avaaaaatak iqamdavann alalglsspg
      301 avlsssaaal glsspgvigs ssaaavippp gltiptlvpp glvgppgvlp qpvlaanapg
      361 iitgvtpmpp taaalippps ivttlplaaa kpatdllgaa alatttaave talsqatitq
      421 aalnqvvlnq aalkqpvvsq apateapkee ddlakkiaee lepqtlqqqe nmmikgsnar
      481 hmlmrklmrr tescvvvlrn mvgvedldde lesevtdecg rfgsvkrvii yqerqseden
      541 aeivvkifve fsqaqesasa rdalngrffg grivkaelyd qtlyeandls g
//
LOCUS       XP_077499512             591 aa            linear   INV 25-NOV-2025
DEFINITION  poly(U)-binding-splicing factor hfp isoform X10 [Amblyomma
            americanum].
ACCESSION   XP_077499512
VERSION     XP_077499512.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643386.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..591
                     /product="poly(U)-binding-splicing factor hfp isoform X10"
                     /calculated_mol_wt=62755
     Region          2..591
                     /region_name="half-pint"
                     /note="poly-U binding splicing factor, half-pint family;
                     TIGR01645"
                     /db_xref="CDD:130706"
     CDS             1..591
                     /gene="hfp"
                     /coded_by="XM_077643386.1:198..1973"
                     /db_xref="GeneID:144110401"
ORIGIN      
        1 mgpvlagpga lkaappligf glmklspeql emvtrakkfa meqsiksvlm kqtiahqqqq
       61 qktlqrhqal vlmcrwcrvy vgsisfelke dtikqafrpf gpiksinmsw dpvtqkhkgf
      121 afveyelpea aqlaleqmng vliggrnikv grpsnmpqaa pildqimeea ktynriyias
      181 vhqdltesdi qsvfeafgki rscklipsst pgkhkeygfi eyetnvgane aiasmnlfdl
      241 ggqylrvgra itppnaltpp saqsvmptaa avaaaaatak iqamdavann alalglsspg
      301 avlsssaaal glsspgvigs ssaaavippp gltiptlvpp glvgppgvlp qpvlaanapg
      361 iitgvtpmpp taaalippps ivttlplaaa kpatdllgaa alatttaave talsqatitq
      421 aalnqvvlnq aalkqpvvsq apateapkee ddlakkiaee lepqtlqqqe nmmikgsnar
      481 hmlmrklmrr tescvvvlrn mvgvedldde lesevtdecg rfgsvkrvii yqerqseden
      541 aeivvkifve fsqaqesasa rdalngrffg grivkaelyd qtlyeandls g
//
LOCUS       XP_077499519             588 aa            linear   INV 25-NOV-2025
DEFINITION  poly(U)-binding-splicing factor hfp isoform X11 [Amblyomma
            americanum].
ACCESSION   XP_077499519
VERSION     XP_077499519.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643393.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..588
                     /product="poly(U)-binding-splicing factor hfp isoform X11"
                     /calculated_mol_wt=62309
     Region          2..588
                     /region_name="half-pint"
                     /note="poly-U binding splicing factor, half-pint family;
                     TIGR01645"
                     /db_xref="CDD:130706"
     CDS             1..588
                     /gene="hfp"
                     /coded_by="XM_077643393.1:696..2462"
                     /db_xref="GeneID:144110401"
ORIGIN      
        1 mgpvlagpga lkaappligf glmklspeql emvtrakkfa meqsiksvlm kqtiahqqqq
       61 qktlqrhqal vlmcrvyvgs isfelkedti kqafrpfgpi ksinmswdpv tqkhkgfafv
      121 eyelpeaaql aleqmngvli ggrnikvgrp snmpqaapil dqimeeakty nriyiasvhq
      181 dltesdiqsv feafgkirsc klipsstpgk hkeygfieye tnvganeaia smnlfdlggq
      241 ylrvgraitp pnaltppsaq svmptaaava aaaatakiqa mdavannala lglsspgavl
      301 sssaaalgls spgvigsssa aavipppglt iptlvppglv gppgvlpqpv laanapgiit
      361 gvtpmpptaa alipppsivt tlplaaakpa tdllgaaala tttaavetal sqatitqaal
      421 nqvvlnqaal kqpvvsqapa teapkeeddl akkiaeelep qtlqqqenmm ikgsnarhml
      481 mrklmrrtes cvvvlrnmvg vedlddeles evtdecgrfg svkrviiyqe rqsedenaei
      541 vvkifvefsq aqesasarda lngrffggri vkaelydqtl yeandlsg
//
LOCUS       XP_077499528             588 aa            linear   INV 25-NOV-2025
DEFINITION  poly(U)-binding-splicing factor hfp isoform X11 [Amblyomma
            americanum].
ACCESSION   XP_077499528
VERSION     XP_077499528.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643402.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..588
                     /product="poly(U)-binding-splicing factor hfp isoform X11"
                     /calculated_mol_wt=62309
     Region          2..588
                     /region_name="half-pint"
                     /note="poly-U binding splicing factor, half-pint family;
                     TIGR01645"
                     /db_xref="CDD:130706"
     CDS             1..588
                     /gene="hfp"
                     /coded_by="XM_077643402.1:606..2372"
                     /db_xref="GeneID:144110401"
ORIGIN      
        1 mgpvlagpga lkaappligf glmklspeql emvtrakkfa meqsiksvlm kqtiahqqqq
       61 qktlqrhqal vlmcrvyvgs isfelkedti kqafrpfgpi ksinmswdpv tqkhkgfafv
      121 eyelpeaaql aleqmngvli ggrnikvgrp snmpqaapil dqimeeakty nriyiasvhq
      181 dltesdiqsv feafgkirsc klipsstpgk hkeygfieye tnvganeaia smnlfdlggq
      241 ylrvgraitp pnaltppsaq svmptaaava aaaatakiqa mdavannala lglsspgavl
      301 sssaaalgls spgvigsssa aavipppglt iptlvppglv gppgvlpqpv laanapgiit
      361 gvtpmpptaa alipppsivt tlplaaakpa tdllgaaala tttaavetal sqatitqaal
      421 nqvvlnqaal kqpvvsqapa teapkeeddl akkiaeelep qtlqqqenmm ikgsnarhml
      481 mrklmrrtes cvvvlrnmvg vedlddeles evtdecgrfg svkrviiyqe rqsedenaei
      541 vvkifvefsq aqesasarda lngrffggri vkaelydqtl yeandlsg
//
LOCUS       XP_077499536             588 aa            linear   INV 25-NOV-2025
DEFINITION  poly(U)-binding-splicing factor hfp isoform X11 [Amblyomma
            americanum].
ACCESSION   XP_077499536
VERSION     XP_077499536.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643410.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..588
                     /product="poly(U)-binding-splicing factor hfp isoform X11"
                     /calculated_mol_wt=62309
     Region          2..588
                     /region_name="half-pint"
                     /note="poly-U binding splicing factor, half-pint family;
                     TIGR01645"
                     /db_xref="CDD:130706"
     CDS             1..588
                     /gene="hfp"
                     /coded_by="XM_077643410.1:989..2755"
                     /db_xref="GeneID:144110401"
ORIGIN      
        1 mgpvlagpga lkaappligf glmklspeql emvtrakkfa meqsiksvlm kqtiahqqqq
       61 qktlqrhqal vlmcrvyvgs isfelkedti kqafrpfgpi ksinmswdpv tqkhkgfafv
      121 eyelpeaaql aleqmngvli ggrnikvgrp snmpqaapil dqimeeakty nriyiasvhq
      181 dltesdiqsv feafgkirsc klipsstpgk hkeygfieye tnvganeaia smnlfdlggq
      241 ylrvgraitp pnaltppsaq svmptaaava aaaatakiqa mdavannala lglsspgavl
      301 sssaaalgls spgvigsssa aavipppglt iptlvppglv gppgvlpqpv laanapgiit
      361 gvtpmpptaa alipppsivt tlplaaakpa tdllgaaala tttaavetal sqatitqaal
      421 nqvvlnqaal kqpvvsqapa teapkeeddl akkiaeelep qtlqqqenmm ikgsnarhml
      481 mrklmrrtes cvvvlrnmvg vedlddeles evtdecgrfg svkrviiyqe rqsedenaei
      541 vvkifvefsq aqesasarda lngrffggri vkaelydqtl yeandlsg
//
LOCUS       XP_077499547             586 aa            linear   INV 25-NOV-2025
DEFINITION  poly(U)-binding-splicing factor hfp isoform X12 [Amblyomma
            americanum].
ACCESSION   XP_077499547
VERSION     XP_077499547.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643421.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..586
                     /product="poly(U)-binding-splicing factor hfp isoform X12"
                     /calculated_mol_wt=62053
     Region          2..586
                     /region_name="half-pint"
                     /note="poly-U binding splicing factor, half-pint family;
                     TIGR01645"
                     /db_xref="CDD:130706"
     CDS             1..586
                     /gene="hfp"
                     /coded_by="XM_077643421.1:506..2266"
                     /db_xref="GeneID:144110401"
ORIGIN      
        1 mgpvlagpga lkaappligf glmklspeql emvtrakkfa meqsiksvlm kqtiahqqqk
       61 tlqrhqalvl mcrvyvgsis felkedtikq afrpfgpiks inmswdpvtq khkgfafvey
      121 elpeaaqlal eqmngvligg rnikvgrpsn mpqaapildq imeeaktynr iyiasvhqdl
      181 tesdiqsvfe afgkirsckl ipsstpgkhk eygfieyetn vganeaiasm nlfdlggqyl
      241 rvgraitppn altppsaqsv mptaaavaaa aatakiqamd avannalalg lsspgavlss
      301 saaalglssp gvigsssaaa vipppgltip tlvppglvgp pgvlpqpvla anapgiitgv
      361 tpmpptaaal ipppsivttl plaaakpatd llgaaalatt taavetalsq atitqaalnq
      421 vvlnqaalkq pvvsqapate apkeeddlak kiaeelepqt lqqqenmmik gsnarhmlmr
      481 klmrrtescv vvlrnmvgve dlddelesev tdecgrfgsv krviiyqerq sedenaeivv
      541 kifvefsqaq esasardaln grffggrivk aelydqtlye andlsg
//
LOCUS       XP_077499556             572 aa            linear   INV 25-NOV-2025
DEFINITION  poly(U)-binding-splicing factor hfp isoform X13 [Amblyomma
            americanum].
ACCESSION   XP_077499556
VERSION     XP_077499556.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643430.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..572
                     /product="poly(U)-binding-splicing factor hfp isoform X13"
                     /calculated_mol_wt=60940
     Region          2..572
                     /region_name="half-pint"
                     /note="poly-U binding splicing factor, half-pint family;
                     TIGR01645"
                     /db_xref="CDD:130706"
     CDS             1..572
                     /gene="hfp"
                     /coded_by="XM_077643430.1:694..2412"
                     /db_xref="GeneID:144110401"
ORIGIN      
        1 mgpvlagpga lkaappligf glmklspeql emvtrakkfa meqsiksvlm kqtiahqqqq
       61 qktlqrhqal vlmcrvyvgs isfelkedti kqafrpfgpi ksinmswdpv tqkhkgfafv
      121 eyelpeaaql aleqmngvli ggrnikvgrp snmpqaapil dqimeeakty nriyiasvhq
      181 dltesdiqsv feafgkirsc klipsstpgk hkeygfieye tnvganeaia smnlfdlggq
      241 ylrvgraitp pnaltppsaq svmptaaava aaaatakiqa mdavannala lglsspgvig
      301 sssaaavipp pgltiptlvp pglvgppgvl pqpvlaanap giitgvtpmp ptaaalippp
      361 sivttlplaa akpatdllga aalatttaav etalsqatit qaalnqvvln qaalkqpvvs
      421 qapateapke eddlakkiae elepqtlqqq enmmikgsna rhmlmrklmr rtescvvvlr
      481 nmvgvedldd elesevtdec grfgsvkrvi iyqerqsede naeivvkifv efsqaqesas
      541 ardalngrff ggrivkaely dqtlyeandl sg
//
LOCUS       XP_077499567             572 aa            linear   INV 25-NOV-2025
DEFINITION  poly(U)-binding-splicing factor hfp isoform X13 [Amblyomma
            americanum].
ACCESSION   XP_077499567
VERSION     XP_077499567.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643441.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..572
                     /product="poly(U)-binding-splicing factor hfp isoform X13"
                     /calculated_mol_wt=60940
     Region          2..572
                     /region_name="half-pint"
                     /note="poly-U binding splicing factor, half-pint family;
                     TIGR01645"
                     /db_xref="CDD:130706"
     CDS             1..572
                     /gene="hfp"
                     /coded_by="XM_077643441.1:631..2349"
                     /db_xref="GeneID:144110401"
ORIGIN      
        1 mgpvlagpga lkaappligf glmklspeql emvtrakkfa meqsiksvlm kqtiahqqqq
       61 qktlqrhqal vlmcrvyvgs isfelkedti kqafrpfgpi ksinmswdpv tqkhkgfafv
      121 eyelpeaaql aleqmngvli ggrnikvgrp snmpqaapil dqimeeakty nriyiasvhq
      181 dltesdiqsv feafgkirsc klipsstpgk hkeygfieye tnvganeaia smnlfdlggq
      241 ylrvgraitp pnaltppsaq svmptaaava aaaatakiqa mdavannala lglsspgvig
      301 sssaaavipp pgltiptlvp pglvgppgvl pqpvlaanap giitgvtpmp ptaaalippp
      361 sivttlplaa akpatdllga aalatttaav etalsqatit qaalnqvvln qaalkqpvvs
      421 qapateapke eddlakkiae elepqtlqqq enmmikgsna rhmlmrklmr rtescvvvlr
      481 nmvgvedldd elesevtdec grfgsvkrvi iyqerqsede naeivvkifv efsqaqesas
      541 ardalngrff ggrivkaely dqtlyeandl sg
//
LOCUS       XP_077499576             572 aa            linear   INV 25-NOV-2025
DEFINITION  poly(U)-binding-splicing factor hfp isoform X13 [Amblyomma
            americanum].
ACCESSION   XP_077499576
VERSION     XP_077499576.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643450.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..572
                     /product="poly(U)-binding-splicing factor hfp isoform X13"
                     /calculated_mol_wt=60940
     Region          2..572
                     /region_name="half-pint"
                     /note="poly-U binding splicing factor, half-pint family;
                     TIGR01645"
                     /db_xref="CDD:130706"
     CDS             1..572
                     /gene="hfp"
                     /coded_by="XM_077643450.1:563..2281"
                     /db_xref="GeneID:144110401"
ORIGIN      
        1 mgpvlagpga lkaappligf glmklspeql emvtrakkfa meqsiksvlm kqtiahqqqq
       61 qktlqrhqal vlmcrvyvgs isfelkedti kqafrpfgpi ksinmswdpv tqkhkgfafv
      121 eyelpeaaql aleqmngvli ggrnikvgrp snmpqaapil dqimeeakty nriyiasvhq
      181 dltesdiqsv feafgkirsc klipsstpgk hkeygfieye tnvganeaia smnlfdlggq
      241 ylrvgraitp pnaltppsaq svmptaaava aaaatakiqa mdavannala lglsspgvig
      301 sssaaavipp pgltiptlvp pglvgppgvl pqpvlaanap giitgvtpmp ptaaalippp
      361 sivttlplaa akpatdllga aalatttaav etalsqatit qaalnqvvln qaalkqpvvs
      421 qapateapke eddlakkiae elepqtlqqq enmmikgsna rhmlmrklmr rtescvvvlr
      481 nmvgvedldd elesevtdec grfgsvkrvi iyqerqsede naeivvkifv efsqaqesas
      541 ardalngrff ggrivkaely dqtlyeandl sg
//
LOCUS       XP_077499585             572 aa            linear   INV 25-NOV-2025
DEFINITION  poly(U)-binding-splicing factor hfp isoform X13 [Amblyomma
            americanum].
ACCESSION   XP_077499585
VERSION     XP_077499585.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643459.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..572
                     /product="poly(U)-binding-splicing factor hfp isoform X13"
                     /calculated_mol_wt=60940
     Region          2..572
                     /region_name="half-pint"
                     /note="poly-U binding splicing factor, half-pint family;
                     TIGR01645"
                     /db_xref="CDD:130706"
     CDS             1..572
                     /gene="hfp"
                     /coded_by="XM_077643459.1:498..2216"
                     /db_xref="GeneID:144110401"
ORIGIN      
        1 mgpvlagpga lkaappligf glmklspeql emvtrakkfa meqsiksvlm kqtiahqqqq
       61 qktlqrhqal vlmcrvyvgs isfelkedti kqafrpfgpi ksinmswdpv tqkhkgfafv
      121 eyelpeaaql aleqmngvli ggrnikvgrp snmpqaapil dqimeeakty nriyiasvhq
      181 dltesdiqsv feafgkirsc klipsstpgk hkeygfieye tnvganeaia smnlfdlggq
      241 ylrvgraitp pnaltppsaq svmptaaava aaaatakiqa mdavannala lglsspgvig
      301 sssaaavipp pgltiptlvp pglvgppgvl pqpvlaanap giitgvtpmp ptaaalippp
      361 sivttlplaa akpatdllga aalatttaav etalsqatit qaalnqvvln qaalkqpvvs
      421 qapateapke eddlakkiae elepqtlqqq enmmikgsna rhmlmrklmr rtescvvvlr
      481 nmvgvedldd elesevtdec grfgsvkrvi iyqerqsede naeivvkifv efsqaqesas
      541 ardalngrff ggrivkaely dqtlyeandl sg
//
LOCUS       XP_077499594             572 aa            linear   INV 25-NOV-2025
DEFINITION  poly(U)-binding-splicing factor hfp isoform X13 [Amblyomma
            americanum].
ACCESSION   XP_077499594
VERSION     XP_077499594.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643468.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..572
                     /product="poly(U)-binding-splicing factor hfp isoform X13"
                     /calculated_mol_wt=60940
     Region          2..572
                     /region_name="half-pint"
                     /note="poly-U binding splicing factor, half-pint family;
                     TIGR01645"
                     /db_xref="CDD:130706"
     CDS             1..572
                     /gene="hfp"
                     /coded_by="XM_077643468.1:433..2151"
                     /db_xref="GeneID:144110401"
ORIGIN      
        1 mgpvlagpga lkaappligf glmklspeql emvtrakkfa meqsiksvlm kqtiahqqqq
       61 qktlqrhqal vlmcrvyvgs isfelkedti kqafrpfgpi ksinmswdpv tqkhkgfafv
      121 eyelpeaaql aleqmngvli ggrnikvgrp snmpqaapil dqimeeakty nriyiasvhq
      181 dltesdiqsv feafgkirsc klipsstpgk hkeygfieye tnvganeaia smnlfdlggq
      241 ylrvgraitp pnaltppsaq svmptaaava aaaatakiqa mdavannala lglsspgvig
      301 sssaaavipp pgltiptlvp pglvgppgvl pqpvlaanap giitgvtpmp ptaaalippp
      361 sivttlplaa akpatdllga aalatttaav etalsqatit qaalnqvvln qaalkqpvvs
      421 qapateapke eddlakkiae elepqtlqqq enmmikgsna rhmlmrklmr rtescvvvlr
      481 nmvgvedldd elesevtdec grfgsvkrvi iyqerqsede naeivvkifv efsqaqesas
      541 ardalngrff ggrivkaely dqtlyeandl sg
//
LOCUS       XP_077499601             572 aa            linear   INV 25-NOV-2025
DEFINITION  poly(U)-binding-splicing factor hfp isoform X13 [Amblyomma
            americanum].
ACCESSION   XP_077499601
VERSION     XP_077499601.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643475.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..572
                     /product="poly(U)-binding-splicing factor hfp isoform X13"
                     /calculated_mol_wt=60940
     Region          2..572
                     /region_name="half-pint"
                     /note="poly-U binding splicing factor, half-pint family;
                     TIGR01645"
                     /db_xref="CDD:130706"
     CDS             1..572
                     /gene="hfp"
                     /coded_by="XM_077643475.1:541..2259"
                     /db_xref="GeneID:144110401"
ORIGIN      
        1 mgpvlagpga lkaappligf glmklspeql emvtrakkfa meqsiksvlm kqtiahqqqq
       61 qktlqrhqal vlmcrvyvgs isfelkedti kqafrpfgpi ksinmswdpv tqkhkgfafv
      121 eyelpeaaql aleqmngvli ggrnikvgrp snmpqaapil dqimeeakty nriyiasvhq
      181 dltesdiqsv feafgkirsc klipsstpgk hkeygfieye tnvganeaia smnlfdlggq
      241 ylrvgraitp pnaltppsaq svmptaaava aaaatakiqa mdavannala lglsspgvig
      301 sssaaavipp pgltiptlvp pglvgppgvl pqpvlaanap giitgvtpmp ptaaalippp
      361 sivttlplaa akpatdllga aalatttaav etalsqatit qaalnqvvln qaalkqpvvs
      421 qapateapke eddlakkiae elepqtlqqq enmmikgsna rhmlmrklmr rtescvvvlr
      481 nmvgvedldd elesevtdec grfgsvkrvi iyqerqsede naeivvkifv efsqaqesas
      541 ardalngrff ggrivkaely dqtlyeandl sg
//
LOCUS       XP_077499609             570 aa            linear   INV 25-NOV-2025
DEFINITION  poly(U)-binding-splicing factor hfp isoform X14 [Amblyomma
            americanum].
ACCESSION   XP_077499609
VERSION     XP_077499609.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643483.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..570
                     /product="poly(U)-binding-splicing factor hfp isoform X14"
                     /calculated_mol_wt=60683
     Region          2..570
                     /region_name="half-pint"
                     /note="poly-U binding splicing factor, half-pint family;
                     TIGR01645"
                     /db_xref="CDD:130706"
     CDS             1..570
                     /gene="hfp"
                     /coded_by="XM_077643483.1:284..1996"
                     /db_xref="GeneID:144110401"
ORIGIN      
        1 mgpvlagpga lkaappligf glmklspeql emvtrakkfa meqsiksvlm kqtiahqqqk
       61 tlqrhqalvl mcrvyvgsis felkedtikq afrpfgpiks inmswdpvtq khkgfafvey
      121 elpeaaqlal eqmngvligg rnikvgrpsn mpqaapildq imeeaktynr iyiasvhqdl
      181 tesdiqsvfe afgkirsckl ipsstpgkhk eygfieyetn vganeaiasm nlfdlggqyl
      241 rvgraitppn altppsaqsv mptaaavaaa aatakiqamd avannalalg lsspgvigss
      301 saaavipppg ltiptlvppg lvgppgvlpq pvlaanapgi itgvtpmppt aaalipppsi
      361 vttlplaaak patdllgaaa latttaavet alsqatitqa alnqvvlnqa alkqpvvsqa
      421 pateapkeed dlakkiaeel epqtlqqqen mmikgsnarh mlmrklmrrt escvvvlrnm
      481 vgvedlddel esevtdecgr fgsvkrviiy qerqsedena eivvkifvef sqaqesasar
      541 dalngrffgg rivkaelydq tlyeandlsg
//
LOCUS       XP_077499614             591 aa            linear   INV 25-NOV-2025
DEFINITION  poly(U)-binding-splicing factor hfp isoform X10 [Amblyomma
            americanum].
ACCESSION   XP_077499614
VERSION     XP_077499614.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643488.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..591
                     /product="poly(U)-binding-splicing factor hfp isoform X10"
                     /calculated_mol_wt=62755
     Region          2..591
                     /region_name="half-pint"
                     /note="poly-U binding splicing factor, half-pint family;
                     TIGR01645"
                     /db_xref="CDD:130706"
     CDS             1..591
                     /gene="hfp"
                     /coded_by="XM_077643488.1:158..1933"
                     /db_xref="GeneID:144110401"
ORIGIN      
        1 mgpvlagpga lkaappligf glmklspeql emvtrakkfa meqsiksvlm kqtiahqqqq
       61 qktlqrhqal vlmcrwcrvy vgsisfelke dtikqafrpf gpiksinmsw dpvtqkhkgf
      121 afveyelpea aqlaleqmng vliggrnikv grpsnmpqaa pildqimeea ktynriyias
      181 vhqdltesdi qsvfeafgki rscklipsst pgkhkeygfi eyetnvgane aiasmnlfdl
      241 ggqylrvgra itppnaltpp saqsvmptaa avaaaaatak iqamdavann alalglsspg
      301 avlsssaaal glsspgvigs ssaaavippp gltiptlvpp glvgppgvlp qpvlaanapg
      361 iitgvtpmpp taaalippps ivttlplaaa kpatdllgaa alatttaave talsqatitq
      421 aalnqvvlnq aalkqpvvsq apateapkee ddlakkiaee lepqtlqqqe nmmikgsnar
      481 hmlmrklmrr tescvvvlrn mvgvedldde lesevtdecg rfgsvkrvii yqerqseden
      541 aeivvkifve fsqaqesasa rdalngrffg grivkaelyd qtlyeandls g
//
LOCUS       XP_077499628             911 aa            linear   INV 25-NOV-2025
DEFINITION  protein FAM184A-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077499628
VERSION     XP_077499628.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643502.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..911
                     /product="protein FAM184A-like isoform X1"
                     /calculated_mol_wt=102945
     Region          39..226
                     /region_name="FAM184"
                     /note="Family with sequence similarity 184, A and B;
                     pfam15665"
                     /db_xref="CDD:464788"
     Region          <217..>744
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..911
                     /gene="LOC144110515"
                     /coded_by="XM_077643502.1:202..2937"
                     /db_xref="GeneID:144110515"
ORIGIN      
        1 magrrcprga hadmkmpadc mspelfakma rkiaqltkvv ytlstrvedq ealmlamkql
       61 heedvqrvvr danekvarcq sqvsrqvall eqlegalvhe rqercrlege lhrrllqpts
      121 tggqllalca elrdaglalr skldaferlq lhvalalegv pvsgglples slsralvdgq
      181 adddcvlrkn dflrdhcdql aeenermrsh fegqlaelrs fyeeslsklq ssrrleland
      241 asllaccaek ettaercrmd alllqlahse qqvdryckea kalsdelekr dkhlkvmddq
      301 vsgsqeelqr ltgrlkqsea alhqlrtsca lhkqdgiaka driasleata geqqsrleql
      361 ladaeqlapl rdecsrlreq lavakgavak hqadsqrels akqhkleele rrlntfrdes
      421 kclkliqaql kdslfesenk vsqltedldl ktqalnaker hanalwedvk klkwevteai
      481 eekeallses rrlldrtqre innawesklk selatqralg qldddrallq grateertar
      541 vvalserlea qgqqaqcdrr rleerleqta adgaatlkrq heghnrlwlp kvsqgelgrq
      601 avhrqtfrvr pdeelfqeqt adgsesrmad rhlkyaeeqd kllrdsgtdg rrvlgchfnh
      661 safernnvdh sgniselknl vraqqenlga vsleldskke qvlrirseat qqiknlelyw
      721 qrritkelnd sctrheqekk flqenyaktl tllkdriaal ekraqgevss sakhldlcqv
      781 epyaervkae dgrqvleerl pplnrspvsg ydsetsqara psaavrmptc tatalcsprl
      841 lkktapefsg gppphaprrt pswakppval pprtaelqsk rsvwfsappa dagrtavlrr
      901 vpegarhsrq q
//
LOCUS       XP_077499634             911 aa            linear   INV 25-NOV-2025
DEFINITION  protein FAM184A-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077499634
VERSION     XP_077499634.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643508.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..911
                     /product="protein FAM184A-like isoform X2"
                     /calculated_mol_wt=102862
     Region          39..226
                     /region_name="FAM184"
                     /note="Family with sequence similarity 184, A and B;
                     pfam15665"
                     /db_xref="CDD:464788"
     Region          <217..>744
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..911
                     /gene="LOC144110515"
                     /coded_by="XM_077643508.1:203..2938"
                     /db_xref="GeneID:144110515"
ORIGIN      
        1 magrrcprga hadmkmpadc mspelfakma rkiaqltkvv ytlstrvedq ealmlamkql
       61 heedvqrvvr danekvarcq sqvsrqvall eqlegalvhe rqercrlege lhrrllqpts
      121 tggqllalca elrdaglalr skldaferlq lhvalalegv pvsgglples slsralvdgq
      181 adddcvlrkn dflrdhcdql aeenermrsh fegqlaelrs fyeeslsklq ssrrleland
      241 asllaccaek ettaercrmd alllqlahse qqvdryckea kalsdelekr dkhlkvmddq
      301 vsgsqeelqr ltgrlkqsea alhqlrtsca lhkqdgiaka gmiasleata geqqsrleql
      361 ladaeqlapl rdecsrlreq lavakgavak hqadsqrels akqhkleele rrlntfrdes
      421 kclkliqaql kdslfesenk vsqltedldl ktqalnaker hanalwedvk klkwevteai
      481 eekeallses rrlldrtqre innawesklk selatqralg qldddrallq grateertar
      541 vvalserlea qgqqaqcdrr rleerleqta adgaatlkrq heghnrlwlp kvsqgelgrq
      601 avhrqtfrvr pdeelfqeqt adgsesrmad rhlkyaeeqd kllrdsgtdg rrvlgchfnh
      661 safernnvdh sgniselknl vraqqenlga vsleldskke qvlrirseat qqiknlelyw
      721 qrritkelnd sctrheqekk flqenyaktl tllkdriaal ekraqgevss sakhldlcqv
      781 epyaervkae dgrqvleerl pplnrspvsg ydsetsqara psaavrmptc tatalcsprl
      841 lkktapefsg gppphaprrt pswakppval pprtaelqsk rsvwfsappa dagrtavlrr
      901 vpegarhsrq q
//
LOCUS       XP_077499643             894 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144110515 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077499643
VERSION     XP_077499643.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643517.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..894
                     /product="uncharacterized protein LOC144110515 isoform X3"
                     /calculated_mol_wt=101109
     Region          22..209
                     /region_name="FAM184"
                     /note="Family with sequence similarity 184, A and B;
                     pfam15665"
                     /db_xref="CDD:464788"
     Region          <200..>727
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..894
                     /gene="LOC144110515"
                     /coded_by="XM_077643517.1:146..2830"
                     /db_xref="GeneID:144110515"
ORIGIN      
        1 mrviggshcl pwplrllctt mvvytlstrv edqealmlam kqlheedvqr vvrdanekva
       61 rcqsqvsrqv alleqlegal vherqercrl egelhrrllq ptstggqlla lcaelrdagl
      121 alrskldafe rlqlhvalal egvpvsgglp lesslsralv dgqadddcvl rkndflrdhc
      181 dqlaeenerm rshfegqlae lrsfyeesls klqssrrlel andasllacc aekettaerc
      241 rmdalllqla hseqqvdryc keakalsdel ekrdkhlkvm ddqvsgsqee lqrltgrlkq
      301 seaalhqlrt scalhkqdgi akadriasle atageqqsrl eqlladaeql aplrdecsrl
      361 reqlavakga vakhqadsqr elsakqhkle elerrlntfr deskclkliq aqlkdslfes
      421 enkvsqlted ldlktqalna kerhanalwe dvkklkwevt eaieekeall sesrrlldrt
      481 qreinnawes klkselatqr algqldddra llqgrateer tarvvalser leaqgqqaqc
      541 drrrleerle qtaadgaatl krqheghnrl wlpkvsqgel grqavhrqtf rvrpdeelfq
      601 eqtadgsesr madrhlkyae eqdkllrdsg tdgrrvlgch fnhsafernn vdhsgnisel
      661 knlvraqqen lgavslelds kkeqvlrirs eatqqiknle lywqrritke lndsctrheq
      721 ekkflqenya ktltllkdri aalekraqge vsssakhldl cqvepyaerv kaedgrqvle
      781 erlpplnrsp vsgydsetsq arapsaavrm ptctatalcs prllkktape fsggppphap
      841 rrtpswakpp valpprtael qskrsvwfsa ppadagrtav lrrvpegarh srqq
//
LOCUS       XP_077499652             894 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144110515 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077499652
VERSION     XP_077499652.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643526.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..894
                     /product="uncharacterized protein LOC144110515 isoform X3"
                     /calculated_mol_wt=101109
     Region          22..209
                     /region_name="FAM184"
                     /note="Family with sequence similarity 184, A and B;
                     pfam15665"
                     /db_xref="CDD:464788"
     Region          <200..>727
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..894
                     /gene="LOC144110515"
                     /coded_by="XM_077643526.1:119..2803"
                     /db_xref="GeneID:144110515"
ORIGIN      
        1 mrviggshcl pwplrllctt mvvytlstrv edqealmlam kqlheedvqr vvrdanekva
       61 rcqsqvsrqv alleqlegal vherqercrl egelhrrllq ptstggqlla lcaelrdagl
      121 alrskldafe rlqlhvalal egvpvsgglp lesslsralv dgqadddcvl rkndflrdhc
      181 dqlaeenerm rshfegqlae lrsfyeesls klqssrrlel andasllacc aekettaerc
      241 rmdalllqla hseqqvdryc keakalsdel ekrdkhlkvm ddqvsgsqee lqrltgrlkq
      301 seaalhqlrt scalhkqdgi akadriasle atageqqsrl eqlladaeql aplrdecsrl
      361 reqlavakga vakhqadsqr elsakqhkle elerrlntfr deskclkliq aqlkdslfes
      421 enkvsqlted ldlktqalna kerhanalwe dvkklkwevt eaieekeall sesrrlldrt
      481 qreinnawes klkselatqr algqldddra llqgrateer tarvvalser leaqgqqaqc
      541 drrrleerle qtaadgaatl krqheghnrl wlpkvsqgel grqavhrqtf rvrpdeelfq
      601 eqtadgsesr madrhlkyae eqdkllrdsg tdgrrvlgch fnhsafernn vdhsgnisel
      661 knlvraqqen lgavslelds kkeqvlrirs eatqqiknle lywqrritke lndsctrheq
      721 ekkflqenya ktltllkdri aalekraqge vsssakhldl cqvepyaerv kaedgrqvle
      781 erlpplnrsp vsgydsetsq arapsaavrm ptctatalcs prllkktape fsggppphap
      841 rrtpswakpp valpprtael qskrsvwfsa ppadagrtav lrrvpegarh srqq
//
LOCUS       XP_077499660             891 aa            linear   INV 25-NOV-2025
DEFINITION  protein FAM184A-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077499660
VERSION     XP_077499660.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643534.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..891
                     /product="protein FAM184A-like isoform X4"
                     /calculated_mol_wt=100587
     Region          39..226
                     /region_name="FAM184"
                     /note="Family with sequence similarity 184, A and B;
                     pfam15665"
                     /db_xref="CDD:464788"
     Region          <217..>744
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..891
                     /gene="LOC144110515"
                     /coded_by="XM_077643534.1:199..2874"
                     /db_xref="GeneID:144110515"
ORIGIN      
        1 magrrcprga hadmkmpadc mspelfakma rkiaqltkvv ytlstrvedq ealmlamkql
       61 heedvqrvvr danekvarcq sqvsrqvall eqlegalvhe rqercrlege lhrrllqpts
      121 tggqllalca elrdaglalr skldaferlq lhvalalegv pvsgglples slsralvdgq
      181 adddcvlrkn dflrdhcdql aeenermrsh fegqlaelrs fyeeslsklq ssrrleland
      241 asllaccaek ettaercrmd alllqlahse qqvdryckea kalsdelekr dkhlkvmddq
      301 vsgsqeelqr ltgrlkqsea alhqlrtsca lhkqdgiaka driasleata geqqsrleql
      361 ladaeqlapl rdecsrlreq lavakgavak hqadsqrels akqhkleele rrlntfrdes
      421 kclkliqaql kdslfesenk vsqltedldl ktqalnaker hanalwedvk klkwevteai
      481 eekeallses rrlldrtqre innawesklk selatqralg qldddrallq grateertar
      541 vvalserlea qgqqaqcdrr rleerleqta adgaatlkrq heghnrlwlp kvsqgelgrq
      601 avhrqtfrvr pdeelfqeqt adgsesrmad rhlkyaeeqd kllrdsgtdg rrvlgchfnh
      661 safernnvdh sgniselknl vraqqenlga vsleldskke qvlrirseat qqiknlelyw
      721 qrritkelnd sctrheqekk flqenyaktl tllkdriaal ekraqgevss sakhldlcqv
      781 epyaespvsg ydsetsqara psaavrmptc tatalcsprl lkktapefsg gppphaprrt
      841 pswakppval pprtaelqsk rsvwfsappa dagrtavlrr vpegarhsrq q
//
LOCUS       XP_077499666             382 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein ZK1073.1 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077499666
VERSION     XP_077499666.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643540.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..382
                     /product="uncharacterized protein ZK1073.1 isoform X1"
                     /calculated_mol_wt=42294
     Region          47..332
                     /region_name="Abhydrolase"
                     /note="alpha/beta hydrolases; cl21494"
                     /db_xref="CDD:473884"
     CDS             1..382
                     /gene="LOC144110538"
                     /coded_by="XM_077643540.1:152..1300"
                     /db_xref="GeneID:144110538"
ORIGIN      
        1 msepanaasp apegapaetq apkgppasrk gclrdiqehe dsimkmevmt kncgkltihv
       61 qgdldnlekk avfltvhdig nnhssfqdfv dhpcmaeikq rsvfihvdvp gqednatelp
      121 sefnfptiqm mgedlisvld hlkinlvvgf gegaganilv rfalahasrv lglilmhlvs
      181 tgvgmmeyfk dkimnwklqn vgmnpsaeqy lvlhkfgaql emvdnkerli sdyteklkkq
      241 inprnlkryv esymnrkdis glieanlktm dvllvtgska ahaqavqnmy armdkqktsl
      301 lkvdavgdvl qespeklaqs lllfvkglgf ltsitlpgve rqrtfsggdh kmlgavgrrh
      361 tlsmedydip rtrrtsltav qk
//
LOCUS       XP_077499677             380 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein ZK1073.1 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077499677
VERSION     XP_077499677.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643551.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..380
                     /product="uncharacterized protein ZK1073.1 isoform X2"
                     /calculated_mol_wt=42141
     Region          45..330
                     /region_name="Abhydrolase"
                     /note="alpha/beta hydrolases; cl21494"
                     /db_xref="CDD:473884"
     CDS             1..380
                     /gene="LOC144110538"
                     /coded_by="XM_077643551.1:152..1294"
                     /db_xref="GeneID:144110538"
ORIGIN      
        1 msepanaasp apegapaetq algtvyeevs cndiqeheds imkmevmtkn cgkltihvqg
       61 dldnlekkav fltvhdignn hssfqdfvdh pcmaeikqrs vfihvdvpgq ednatelpse
      121 fnfptiqmmg edlisvldhl kinlvvgfge gaganilvrf alahasrvlg lilmhlvstg
      181 vgmmeyfkdk imnwklqnvg mnpsaeqylv lhkfgaqlem vdnkerlisd yteklkkqin
      241 prnlkryves ymnrkdisgl ieanlktmdv llvtgskaah aqavqnmyar mdkqktsllk
      301 vdavgdvlqe speklaqsll lfvkglgflt sitlpgverq rtfsggdhkm lgavgrrhtl
      361 smedydiprt rrtsltavqk
//
LOCUS       XP_077499686             368 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein ZK1073.1 isoform X3 [Amblyomma americanum].
ACCESSION   XP_077499686
VERSION     XP_077499686.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643560.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..368
                     /product="uncharacterized protein ZK1073.1 isoform X3"
                     /calculated_mol_wt=40874
     Region          33..318
                     /region_name="Abhydrolase"
                     /note="alpha/beta hydrolases; cl21494"
                     /db_xref="CDD:473884"
     CDS             1..368
                     /gene="LOC144110538"
                     /coded_by="XM_077643560.1:151..1257"
                     /db_xref="GeneID:144110538"
ORIGIN      
        1 msepanaasp apegapaetq diqehedsim kmevmtkncg kltihvqgdl dnlekkavfl
       61 tvhdignnhs sfqdfvdhpc maeikqrsvf ihvdvpgqed natelpsefn fptiqmmged
      121 lisvldhlki nlvvgfgega ganilvrfal ahasrvlgli lmhlvstgvg mmeyfkdkim
      181 nwklqnvgmn psaeqylvlh kfgaqlemvd nkerlisdyt eklkkqinpr nlkryvesym
      241 nrkdisglie anlktmdvll vtgskaahaq avqnmyarmd kqktsllkvd avgdvlqesp
      301 eklaqslllf vkglgfltsi tlpgverqrt fsggdhkmlg avgrrhtlsm edydiprtrr
      361 tsltavqk
//
LOCUS       XP_077499693             358 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein ZK1073.1 isoform X4 [Amblyomma americanum].
ACCESSION   XP_077499693
VERSION     XP_077499693.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643567.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..358
                     /product="uncharacterized protein ZK1073.1 isoform X4"
                     /calculated_mol_wt=39676
     Region          23..308
                     /region_name="Abhydrolase"
                     /note="alpha/beta hydrolases; cl21494"
                     /db_xref="CDD:473884"
     CDS             1..358
                     /gene="LOC144110538"
                     /coded_by="XM_077643567.1:151..1227"
                     /db_xref="GeneID:144110538"
ORIGIN      
        1 msepanaasp apegapaetq kmevmtkncg kltihvqgdl dnlekkavfl tvhdignnhs
       61 sfqdfvdhpc maeikqrsvf ihvdvpgqed natelpsefn fptiqmmged lisvldhlki
      121 nlvvgfgega ganilvrfal ahasrvlgli lmhlvstgvg mmeyfkdkim nwklqnvgmn
      181 psaeqylvlh kfgaqlemvd nkerlisdyt eklkkqinpr nlkryvesym nrkdisglie
      241 anlktmdvll vtgskaahaq avqnmyarmd kqktsllkvd avgdvlqesp eklaqslllf
      301 vkglgfltsi tlpgverqrt fsggdhkmlg avgrrhtlsm edydiprtrr tsltavqk
//
LOCUS       XP_077499701             346 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144110561 [Amblyomma americanum].
ACCESSION   XP_077499701
VERSION     XP_077499701.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643575.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..346
                     /product="uncharacterized protein LOC144110561"
                     /calculated_mol_wt=36583
     CDS             1..346
                     /gene="LOC144110561"
                     /coded_by="XM_077643575.1:1733..2773"
                     /db_xref="GeneID:144110561"
ORIGIN      
        1 mqgredivac gleslpqdrc slppadkrel grhlplvchp vpgacsapsc rwspaiaqgv
       61 syqalaeqla dhfsvaslvg pilalgppaq pspgrpplpa glsssfaglc dapftpyelh
      121 rskrksasga dgltlrmlrn ldapeqpcll dafnsiwdsr ilpeswstai alpirkarkp
      181 prallllacl adlgglqihg vrgpstllln gqclwlplrv pdglpqaply grfhrrrgll
      241 pgggqaargr rplgppgrpg slrqpaplhh qagpgqtqsd ravavirnri sfqplhagsg
      301 wghpelstah hhrgpsglss qalplqpghg rspvrhpags lpcrcp
//
LOCUS       XP_077499742             490 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144110594 [Amblyomma americanum].
ACCESSION   XP_077499742
VERSION     XP_077499742.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643616.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..490
                     /product="uncharacterized protein LOC144110594"
                     /calculated_mol_wt=54581
     CDS             1..490
                     /gene="LOC144110594"
                     /coded_by="XM_077643616.1:241..1713"
                     /db_xref="GeneID:144110594"
ORIGIN      
        1 mfvyieyess lktavvehtq iqtihsnkke afnpldaddf epadtyrvhs cngdnsdcey
       61 vnvkiihmte tleemvlyra krprmsvvqe evdsqkpree gpqpsrpsar rqerghakwv
      121 qrqrliervl hdyemepmpa nnpkalenrl qameeqlsra kpgehgqssp satgsgdsvp
      181 ksaytvlrqk yealqrdiee lraqnsdlek slkskisege gspvgtarsg arectvpvqt
      241 paaqesnghv shtedcaqlg ahsgekrsan nakrgiapst vpastssaek skgytseaed
      301 caqscsrfsf aghtseaedc sqsfahsrae pdldesfntd rpesvynmrg deedpikagt
      361 rredgkvyag cnhwislmdw erlfkaptds nfcaqaasmf wteeelvkls vtgkvsnaav
      421 argtavhrlp llpeklgavk nlfkmytikd afaarrmknv rshlsaflcy krqrhsrrle
      481 ccgatepkcs
//
LOCUS       XP_077499751             151 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144110604 [Amblyomma americanum].
ACCESSION   XP_077499751
VERSION     XP_077499751.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643625.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..151
                     /product="uncharacterized protein LOC144110604"
                     /calculated_mol_wt=16697
     CDS             1..151
                     /gene="LOC144110604"
                     /coded_by="XM_077643625.1:187..642"
                     /db_xref="GeneID:144110604"
ORIGIN      
        1 mqahntqvak digdavvlal wtshqtnrgt rallltgfyy qppvssrclp dpqaaaadwi
       61 ldpcnhctdy lctpqpflea snlmtwqhat isadqdtyir gtvftvqlgh gtagempghs
      121 pfmlfqvnyy yscyrdddvc llavswpppk s
//
LOCUS       XP_077499764             373 aa            linear   INV 25-NOV-2025
DEFINITION  ecdysis triggering hormone receptor [Amblyomma americanum].
ACCESSION   XP_077499764
VERSION     XP_077499764.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643638.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..373
                     /product="ecdysis triggering hormone receptor"
                     /calculated_mol_wt=40729
     Region          38..314
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          58..80
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410628"
     Region          98..120
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410628"
     Region          143..159
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410628"
     Region          189..212
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410628"
     Region          241..263
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410628"
     Region          282..307
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410628"
     CDS             1..373
                     /gene="ETHR"
                     /coded_by="XM_077643638.1:271..1392"
                     /db_xref="GeneID:144110611"
ORIGIN      
        1 mlnasseacg snctlqfprs iqtaatlaig lllalgtvgn llvplvvckt kelrnstnlf
       61 linlsvsdll vllvcmptal velhsqpevw llgegmcrmi pflelvvahv siltilaisf
      121 eryyaicqpl kagytctkmr alaiigaiwl lalavtspml fvsqygwvey ydgsavpscg
      181 tsfgrpwrrl yilasmallf vlpffvlllv yaciarqlvr hralssapsa qaqlrarrqv
      241 vlmlaavvvs ffvcllpyrv filwiiaapg pveaqvslev fysllyvcrv mlylnsainp
      301 ilynaisskf rdafcrllgl rrrrllrqst ttthtstslt fqsslksass lnngatvhsv
      361 vsvgasgapv kpv
//
LOCUS       XP_077499775             650 aa            linear   INV 25-NOV-2025
DEFINITION  WW domain-containing adapter protein with coiled-coil wacky isoform
            X1 [Amblyomma americanum].
ACCESSION   XP_077499775
VERSION     XP_077499775.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643649.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..650
                     /product="WW domain-containing adapter protein with
                     coiled-coil wacky isoform X1"
                     /calculated_mol_wt=70969
     Region          212..238
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     CDS             1..650
                     /gene="wcy"
                     /coded_by="XM_077643649.1:138..2090"
                     /db_xref="GeneID:144110620"
ORIGIN      
        1 mvmharklpr lsdgyfdrnq ahpyensgky ssksnlgsry ndkvrespan gshsrmgspd
       61 srhspryhks synqrlkdrd kdhergyiry vheskyknss seqprghdqq hvprtgssks
      121 pgykdrfskn sslgssssss tkephvsksr padttthrtn nherthvelq pqqqpvqqpv
      181 qqpvqqpvqq plqqpleqpq qqqqlpenll rdwsehvsss gkkyyynckt evsqwekpkd
      241 wdkhhsggsn gssstsslss asssscgssn stqrsatast psvsdpgggr pksrtqdrtp
      301 ttvaspaptt asagttatvt tsnsrqnekh stassnsvvs sstrpyqqpa ttrhqndtrr
      361 ssqsrnqdpr kdaqtqdmdi sppggggstp nstpepsrsq sqpgptmllp qssvtlaqlp
      421 kmmsqlagtr glpklddmsp qeafrtiqqa iqltkqaqst aqveavaare aassspyqgv
      481 hpgrslppiq qqqpvattas gsdkiigdad rrspgsdysa hsarrdspts svsslhslsg
      541 tgslvsavlk psapsltpsl anyynealie hvtgwqadha erqanrysee ahslgslnct
      601 rvsaelkmar slvrlseiqa tvqeqrslfl rqlikeleel ksqvnfmsds
//
LOCUS       XP_077499784             579 aa            linear   INV 25-NOV-2025
DEFINITION  WW domain-containing adapter protein with coiled-coil wacky isoform
            X2 [Amblyomma americanum].
ACCESSION   XP_077499784
VERSION     XP_077499784.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643658.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..579
                     /product="WW domain-containing adapter protein with
                     coiled-coil wacky isoform X2"
                     /calculated_mol_wt=63608
     Region          212..238
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     CDS             1..579
                     /gene="wcy"
                     /coded_by="XM_077643658.1:125..1864"
                     /db_xref="GeneID:144110620"
ORIGIN      
        1 mvmharklpr lsdgyfdrnq ahpyensgky ssksnlgsry ndkvrespan gshsrmgspd
       61 srhspryhks synqrlkdrd kdhergyiry vheskyknss seqprghdqq hvprtgssks
      121 pgykdrfskn sslgssssss tkephvsksr padttthrtn nherthvelq pqqqpvqqpv
      181 qqpvqqpvqq plqqpleqpq qqqqlpenll rdwsehvsss gkkyyynckt evsqwekpkd
      241 wdkhhsggsn gssstsslss asssscgssn stqrsatast psvsdpgggr pksrtqdrtp
      301 ttvaspaptt asagttatvt tsnsrqnekh stassnsvvs sstrpyqqpa ttrhqndtrr
      361 ssqsrnqdpr kafrtiqqai qltkqaqsta qveavaarea assspyqgvh pgrslppiqq
      421 qqpvattasg sdkiigdadr rspgsdysah sarrdsptss vsslhslsgt gslvsavlkp
      481 sapsltpsla nyynealieh vtgwqadhae rqanryseea hslgslnctr vsaelkmars
      541 lvrlseiqat vqeqrslflr qlikeleelk sqvnfmsds
//
LOCUS       XP_077499797             498 aa            linear   INV 25-NOV-2025
DEFINITION  TGF-beta receptor type-1 babo isoform X1 [Amblyomma americanum].
ACCESSION   XP_077499797
VERSION     XP_077499797.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643671.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..498
                     /product="TGF-beta receptor type-1 babo isoform X1"
                     /calculated_mol_wt=55727
     Region          17..96
                     /region_name="TFP_LU_ECD_Babo"
                     /note="extracellular domain (ECD) found in Drosophila
                     melanogaster Baboon and similar proteins; cd23598"
                     /db_xref="CDD:467127"
     Region          172..200
                     /region_name="GS"
                     /note="GS motif; smart00467"
                     /db_xref="CDD:197743"
     Region          204..491
                     /region_name="STKc_TGFbR1_ACVR1b_ACVR1c"
                     /note="Catalytic domain of the Serine/Threonine Kinases,
                     Transforming Growth Factor beta Type I Receptor and
                     Activin Type IB/IC Receptors; cd14143"
                     /db_xref="CDD:271045"
     Site            order(206..210,214,225,227,255,275..278,282,284,328,330,
                     332..333,335,346,349,369..372)
                     /site_type="active"
                     /db_xref="CDD:271045"
     Site            order(206..212,214,225,227,275..276,278,282,332..333,335,
                     346)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271045"
     Site            order(210,282,284,328,330,332,349,369..372)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271045"
     Site            order(237..238,241..242,245..246,260,262)
                     /site_type="other"
                     /note="FKBP12 binding site [polypeptide binding]"
                     /db_xref="CDD:271045"
     Site            345..372
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271045"
     CDS             1..498
                     /gene="babo"
                     /coded_by="XM_077643671.1:552..2048"
                     /db_xref="GeneID:144110630"
ORIGIN      
        1 mlrevlllls vgaasglkcy cdicgaesnn tcetdgicfa attksktgei mhqfrcldkk
       61 kmfppdnpva csksgshnpt nmvlnccsny dfcnrdlrpt fpaateasgd amvgsswpst
      121 helafaiggp iclvcvvlmv ayqlwqqrrr hfryemetsl esepillppg qtikdmiemt
      181 tsgsgsglpl lvqrsiarqi qlvevigkgr ygevwrgrwr gesvavkifs srderswfre
      241 veiyqtvmlr hdnilgfiaa dnkdngtwtq lwlitdyhen gslfdylmri pvdtatmckm
      301 aysianglah lhmeilgtqg kpaiahrdik sknilvkssg acaiadlgla vrydsatnsv
      361 dippnprvgt krylapevld esinmnhfds fkradiyalg lvlweiarrc niggiyedyq
      421 lpyydlvpad ptieemrkav ctdrqrpalp nrwqscealr vmskimkecw yhnsaarlta
      481 lrikktianl gaqedlki
//
LOCUS       XP_077499805             497 aa            linear   INV 25-NOV-2025
DEFINITION  TGF-beta receptor type-1 babo isoform X2 [Amblyomma americanum].
ACCESSION   XP_077499805
VERSION     XP_077499805.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643679.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..497
                     /product="TGF-beta receptor type-1 babo isoform X2"
                     /calculated_mol_wt=55700
     Region          17..93
                     /region_name="TFP_LU_ECD_Babo"
                     /note="extracellular domain (ECD) found in Drosophila
                     melanogaster Baboon and similar proteins; cd23598"
                     /db_xref="CDD:467127"
     Region          171..199
                     /region_name="GS"
                     /note="GS motif; smart00467"
                     /db_xref="CDD:197743"
     Region          203..490
                     /region_name="STKc_TGFbR1_ACVR1b_ACVR1c"
                     /note="Catalytic domain of the Serine/Threonine Kinases,
                     Transforming Growth Factor beta Type I Receptor and
                     Activin Type IB/IC Receptors; cd14143"
                     /db_xref="CDD:271045"
     Site            order(205..209,213,224,226,254,274..277,281,283,327,329,
                     331..332,334,345,348,368..371)
                     /site_type="active"
                     /db_xref="CDD:271045"
     Site            order(205..211,213,224,226,274..275,277,281,331..332,334,
                     345)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271045"
     Site            order(209,281,283,327,329,331,348,368..371)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271045"
     Site            order(236..237,240..241,244..245,259,261)
                     /site_type="other"
                     /note="FKBP12 binding site [polypeptide binding]"
                     /db_xref="CDD:271045"
     Site            344..371
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271045"
     CDS             1..497
                     /gene="babo"
                     /coded_by="XM_077643679.1:418..1911"
                     /db_xref="GeneID:144110630"
ORIGIN      
        1 mlrevlllls vgaasglkcy cdicgaesnn tcetdgicfa attksktgei mhqfrcieke
       61 klippdnpis chhsknvddq faieccrdad ycninivptl sppvlsigda mvgsswpsth
      121 elafaiggpi clvcvvlmva yqlwqqrrrh fryemetsle sepillppgq tikdmiemtt
      181 sgsgsglpll vqrsiarqiq lvevigkgry gevwrgrwrg esvavkifss rderswfrev
      241 eiyqtvmlrh dnilgfiaad nkdngtwtql wlitdyheng slfdylmrip vdtatmckma
      301 ysianglahl hmeilgtqgk paiahrdiks knilvkssga caiadlglav rydsatnsvd
      361 ippnprvgtk rylapevlde sinmnhfdsf kradiyalgl vlweiarrcn iggiyedyql
      421 pyydlvpadp tieemrkavc tdrqrpalpn rwqscealrv mskimkecwy hnsaarltal
      481 rikktianlg aqedlki
//
LOCUS       XP_077499813             488 aa            linear   INV 25-NOV-2025
DEFINITION  TGF-beta receptor type-1 babo isoform X3 [Amblyomma americanum].
ACCESSION   XP_077499813
VERSION     XP_077499813.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643687.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..488
                     /product="TGF-beta receptor type-1 babo isoform X3"
                     /calculated_mol_wt=54843
     Region          7..86
                     /region_name="TFP_LU_ECD_Babo"
                     /note="extracellular domain (ECD) found in Drosophila
                     melanogaster Baboon and similar proteins; cd23598"
                     /db_xref="CDD:467127"
     Region          162..190
                     /region_name="GS"
                     /note="GS motif; smart00467"
                     /db_xref="CDD:197743"
     Region          194..481
                     /region_name="STKc_TGFbR1_ACVR1b_ACVR1c"
                     /note="Catalytic domain of the Serine/Threonine Kinases,
                     Transforming Growth Factor beta Type I Receptor and
                     Activin Type IB/IC Receptors; cd14143"
                     /db_xref="CDD:271045"
     Site            order(196..200,204,215,217,245,265..268,272,274,318,320,
                     322..323,325,336,339,359..362)
                     /site_type="active"
                     /db_xref="CDD:271045"
     Site            order(196..202,204,215,217,265..266,268,272,322..323,325,
                     336)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271045"
     Site            order(200,272,274,318,320,322,339,359..362)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271045"
     Site            order(227..228,231..232,235..236,250,252)
                     /site_type="other"
                     /note="FKBP12 binding site [polypeptide binding]"
                     /db_xref="CDD:271045"
     Site            335..362
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271045"
     CDS             1..488
                     /gene="babo"
                     /coded_by="XM_077643687.1:135..1601"
                     /db_xref="GeneID:144110630"
ORIGIN      
        1 mergprlkcy cdicgaesnn tcetdgicfa attksktgei mhqfrcldkk kmfppdnpva
       61 csksgshnpt nmvlnccsny dfcnrdlrpt fpaateasgd amvgsswpst helafaiggp
      121 iclvcvvlmv ayqlwqqrrr hfryemetsl esepillppg qtikdmiemt tsgsgsglpl
      181 lvqrsiarqi qlvevigkgr ygevwrgrwr gesvavkifs srderswfre veiyqtvmlr
      241 hdnilgfiaa dnkdngtwtq lwlitdyhen gslfdylmri pvdtatmckm aysianglah
      301 lhmeilgtqg kpaiahrdik sknilvkssg acaiadlgla vrydsatnsv dippnprvgt
      361 krylapevld esinmnhfds fkradiyalg lvlweiarrc niggiyedyq lpyydlvpad
      421 ptieemrkav ctdrqrpalp nrwqscealr vmskimkecw yhnsaarlta lrikktianl
      481 gaqedlki
//
LOCUS       XP_077499820             487 aa            linear   INV 25-NOV-2025
DEFINITION  TGF-beta receptor type-1 babo isoform X4 [Amblyomma americanum].
ACCESSION   XP_077499820
VERSION     XP_077499820.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643694.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..487
                     /product="TGF-beta receptor type-1 babo isoform X4"
                     /calculated_mol_wt=54815
     Region          7..83
                     /region_name="TFP_LU_ECD_Babo"
                     /note="extracellular domain (ECD) found in Drosophila
                     melanogaster Baboon and similar proteins; cd23598"
                     /db_xref="CDD:467127"
     Region          161..189
                     /region_name="GS"
                     /note="GS motif; smart00467"
                     /db_xref="CDD:197743"
     Region          193..480
                     /region_name="STKc_TGFbR1_ACVR1b_ACVR1c"
                     /note="Catalytic domain of the Serine/Threonine Kinases,
                     Transforming Growth Factor beta Type I Receptor and
                     Activin Type IB/IC Receptors; cd14143"
                     /db_xref="CDD:271045"
     Site            order(195..199,203,214,216,244,264..267,271,273,317,319,
                     321..322,324,335,338,358..361)
                     /site_type="active"
                     /db_xref="CDD:271045"
     Site            order(195..201,203,214,216,264..265,267,271,321..322,324,
                     335)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271045"
     Site            order(199,271,273,317,319,321,338,358..361)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271045"
     Site            order(226..227,230..231,234..235,249,251)
                     /site_type="other"
                     /note="FKBP12 binding site [polypeptide binding]"
                     /db_xref="CDD:271045"
     Site            334..361
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271045"
     CDS             1..487
                     /gene="babo"
                     /coded_by="XM_077643694.1:174..1637"
                     /db_xref="GeneID:144110630"
ORIGIN      
        1 mergprlkcy cdicgaesnn tcetdgicfa attksktgei mhqfrcieke klippdnpis
       61 chhsknvddq faieccrdad ycninivptl sppvlsigda mvgsswpsth elafaiggpi
      121 clvcvvlmva yqlwqqrrrh fryemetsle sepillppgq tikdmiemtt sgsgsglpll
      181 vqrsiarqiq lvevigkgry gevwrgrwrg esvavkifss rderswfrev eiyqtvmlrh
      241 dnilgfiaad nkdngtwtql wlitdyheng slfdylmrip vdtatmckma ysianglahl
      301 hmeilgtqgk paiahrdiks knilvkssga caiadlglav rydsatnsvd ippnprvgtk
      361 rylapevlde sinmnhfdsf kradiyalgl vlweiarrcn iggiyedyql pyydlvpadp
      421 tieemrkavc tdrqrpalpn rwqscealrv mskimkecwy hnsaarltal rikktianlg
      481 aqedlki
//
LOCUS       XP_077499830             410 aa            linear   INV 25-NOV-2025
DEFINITION  TGF-beta receptor type-1 babo isoform X5 [Amblyomma americanum].
ACCESSION   XP_077499830
VERSION     XP_077499830.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643704.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..410
                     /product="TGF-beta receptor type-1 babo isoform X5"
                     /calculated_mol_wt=46384
     Region          84..112
                     /region_name="GS"
                     /note="GS motif; smart00467"
                     /db_xref="CDD:197743"
     Region          116..403
                     /region_name="STKc_TGFbR1_ACVR1b_ACVR1c"
                     /note="Catalytic domain of the Serine/Threonine Kinases,
                     Transforming Growth Factor beta Type I Receptor and
                     Activin Type IB/IC Receptors; cd14143"
                     /db_xref="CDD:271045"
     Site            order(118..122,126,137,139,167,187..190,194,196,240,242,
                     244..245,247,258,261,281..284)
                     /site_type="active"
                     /db_xref="CDD:271045"
     Site            order(118..124,126,137,139,187..188,190,194,244..245,247,
                     258)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271045"
     Site            order(122,194,196,240,242,244,261,281..284)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271045"
     Site            order(149..150,153..154,157..158,172,174)
                     /site_type="other"
                     /note="FKBP12 binding site [polypeptide binding]"
                     /db_xref="CDD:271045"
     Site            257..284
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271045"
     CDS             1..410
                     /gene="babo"
                     /coded_by="XM_077643704.1:479..1711"
                     /db_xref="GeneID:144110630"
ORIGIN      
        1 meyvspprrn prrvksctns gdamvgsswp sthelafaig gpiclvcvvl mvayqlwqqr
       61 rrhfryemet slesepillp pgqtikdmie mttsgsgsgl pllvqrsiar qiqlvevigk
      121 grygevwrgr wrgesvavki fssrderswf reveiyqtvm lrhdnilgfi aadnkdngtw
      181 tqlwlitdyh engslfdylm ripvdtatmc kmaysiangl ahlhmeilgt qgkpaiahrd
      241 iksknilvks sgacaiadlg lavrydsatn svdippnprv gtkrylapev ldesinmnhf
      301 dsfkradiya lglvlweiar rcniggiyed yqlpyydlvp adptieemrk avctdrqrpa
      361 lpnrwqscea lrvmskimke cwyhnsaarl talrikktia nlgaqedlki
//
LOCUS       XP_077499837             410 aa            linear   INV 25-NOV-2025
DEFINITION  TGF-beta receptor type-1 babo isoform X5 [Amblyomma americanum].
ACCESSION   XP_077499837
VERSION     XP_077499837.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643711.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..410
                     /product="TGF-beta receptor type-1 babo isoform X5"
                     /calculated_mol_wt=46384
     Region          84..112
                     /region_name="GS"
                     /note="GS motif; smart00467"
                     /db_xref="CDD:197743"
     Region          116..403
                     /region_name="STKc_TGFbR1_ACVR1b_ACVR1c"
                     /note="Catalytic domain of the Serine/Threonine Kinases,
                     Transforming Growth Factor beta Type I Receptor and
                     Activin Type IB/IC Receptors; cd14143"
                     /db_xref="CDD:271045"
     Site            order(118..122,126,137,139,167,187..190,194,196,240,242,
                     244..245,247,258,261,281..284)
                     /site_type="active"
                     /db_xref="CDD:271045"
     Site            order(118..124,126,137,139,187..188,190,194,244..245,247,
                     258)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271045"
     Site            order(122,194,196,240,242,244,261,281..284)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271045"
     Site            order(149..150,153..154,157..158,172,174)
                     /site_type="other"
                     /note="FKBP12 binding site [polypeptide binding]"
                     /db_xref="CDD:271045"
     Site            257..284
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271045"
     CDS             1..410
                     /gene="babo"
                     /coded_by="XM_077643711.1:193..1425"
                     /db_xref="GeneID:144110630"
ORIGIN      
        1 meyvspprrn prrvksctns gdamvgsswp sthelafaig gpiclvcvvl mvayqlwqqr
       61 rrhfryemet slesepillp pgqtikdmie mttsgsgsgl pllvqrsiar qiqlvevigk
      121 grygevwrgr wrgesvavki fssrderswf reveiyqtvm lrhdnilgfi aadnkdngtw
      181 tqlwlitdyh engslfdylm ripvdtatmc kmaysiangl ahlhmeilgt qgkpaiahrd
      241 iksknilvks sgacaiadlg lavrydsatn svdippnprv gtkrylapev ldesinmnhf
      301 dsfkradiya lglvlweiar rcniggiyed yqlpyydlvp adptieemrk avctdrqrpa
      361 lpnrwqscea lrvmskimke cwyhnsaarl talrikktia nlgaqedlki
//
LOCUS       XP_077499847             517 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144110662 [Amblyomma americanum].
ACCESSION   XP_077499847
VERSION     XP_077499847.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643721.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..517
                     /product="uncharacterized protein LOC144110662"
                     /calculated_mol_wt=58479
     CDS             1..517
                     /gene="LOC144110662"
                     /coded_by="XM_077643721.1:253..1806"
                     /db_xref="GeneID:144110662"
ORIGIN      
        1 mrsctsqddf rmkrglvssc idlfvsfgki aameleqris nwlpaldkei veltvqklqq
       61 cgveslehlk yvveedlqfl kpisrrille rfhsaatpnp pvslspndiq sptstqcasf
      121 stpwevfppq lmkacqegkr pvksdlnemv rlvsdhilai nrkpgrkilr aiaaeivshy
      181 lktfedtlng avigsgietl lwklencvnn krrpsseqph qfepddeldl svkrvkiqrd
      241 sygcvawqpk vpsdetadsq ekkkddllsq frlafpdetm vaqlmsetya sqrqlinask
      301 nirdierswp flfqakhltn hvnillgsnv aktfddrlkt vgrqvfryah sqvkkecvks
      361 clqeieaakt nrksmaveye ampllllaif gedkelfykl teemasdddl gdlpscpfic
      421 vkgwtsltan syticvdthp vlhaskpdea fkllffahfa fniqyqkets lcleftqrai
      481 aginpargtk vqknggkqhc qsprvaalvt alkdydf
//
LOCUS       XP_077499887             411 aa            linear   INV 25-NOV-2025
DEFINITION  TIA1 cytotoxic granule associated RNA binding protein Rox8 isoform
            X1 [Amblyomma americanum].
ACCESSION   XP_077499887
VERSION     XP_077499887.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643761.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..411
                     /product="TIA1 cytotoxic granule associated RNA binding
                     protein Rox8 isoform X1"
                     /calculated_mol_wt=45320
     Region          17..88
                     /region_name="RRM1_TIA1_like"
                     /note="RNA recognition motif 1 (RRM1) found in
                     granule-associated RNA binding proteins p40-TIA-1 and
                     TIAR; cd12352"
                     /db_xref="CDD:409788"
     Region          104..178
                     /region_name="RRM2_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins p40-TIA-1 and
                     TIAR; cd12353"
                     /db_xref="CDD:409789"
     Site            order(113,115,117,121,124,128..129)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:409789"
     Region          215..285
                     /region_name="RRM3_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins (p40-TIA-1 and
                     TIAR), and yeast nuclear and cytoplasmic polyadenylated
                     RNA-binding protein PUB1; cd12354"
                     /db_xref="CDD:409790"
     CDS             1..411
                     /gene="Rox8"
                     /coded_by="XM_077643761.1:1..1236"
                     /db_xref="GeneID:144110701"
ORIGIN      
        1 mfsdfqdqgn dcqprtlyvg nldiavteel lvavfgqmgq vkgckiihep gndpycfvef
       61 sdhqsaasal lamnkrlcfg kemkvnwats pgntpkldts khhhifvgdl speiettqlr
      121 dafapfgdis dcrvvrdpqt lkskgygfvs fvkkadaena igtmngqwlg srairtnwat
      181 rkppanrtqa evdvttstkp ltfdevynqs sptnctvycg gitqglseel mqktfssyga
      241 iqeirvfkdk gyafirfgtk eaathaivat hnsdvngqtv kcswgkeatd pnnqqqpqvs
      301 pgelskhpsw peqrnrsspg nlatqysypy qqmgywypqa gypqvqgqfm qgvqypygqy
      361 ygqgfgsvgm qmawqglpgq pqvaaaqtpm aqglqqpgmi gaypmqqyqa q
//
LOCUS       XP_077499894             409 aa            linear   INV 25-NOV-2025
DEFINITION  TIA1 cytotoxic granule associated RNA binding protein Rox8 isoform
            X2 [Amblyomma americanum].
ACCESSION   XP_077499894
VERSION     XP_077499894.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643768.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..409
                     /product="TIA1 cytotoxic granule associated RNA binding
                     protein Rox8 isoform X2"
                     /calculated_mol_wt=45009
     Region          15..86
                     /region_name="RRM1_TIA1_like"
                     /note="RNA recognition motif 1 (RRM1) found in
                     granule-associated RNA binding proteins p40-TIA-1 and
                     TIAR; cd12352"
                     /db_xref="CDD:409788"
     Region          102..176
                     /region_name="RRM2_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins p40-TIA-1 and
                     TIAR; cd12353"
                     /db_xref="CDD:409789"
     Site            order(111,113,115,119,122,126..127)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:409789"
     Region          213..283
                     /region_name="RRM3_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins (p40-TIA-1 and
                     TIAR), and yeast nuclear and cytoplasmic polyadenylated
                     RNA-binding protein PUB1; cd12354"
                     /db_xref="CDD:409790"
     CDS             1..409
                     /gene="Rox8"
                     /coded_by="XM_077643768.1:66..1295"
                     /db_xref="GeneID:144110701"
ORIGIN      
        1 mankdqgndc qprtlyvgnl diavteellv avfgqmgqvk gckiihepgn dpycfvefsd
       61 hqsaasalla mnkrlcfgke mkvnwatspg ntpkldtskh hhifvgdlsp eiettqlrda
      121 fapfgdisdc rvvrdpqtlk skgygfvsfv kkadaenaig tmngqwlgsr airtnwatrk
      181 ppanrtqaev dvttstkplt fdevynqssp tnctvycggi tqglseelmq ktfssygaiq
      241 eirvfkdkgy afirfgtkea athaivathn sdvngqtvkc swgkeatdpn nqqqpqvspg
      301 elskhpswpe qrnrsspgnl atqysypyqq mgywypqagy pqvqgqfmqg vqypygqyyg
      361 qgfgsvgmqm awqglpgqpq vaaaqtpmaq glqqpgmiga ypmqqyqaq
//
LOCUS       XP_077499903             389 aa            linear   INV 25-NOV-2025
DEFINITION  TIA1 cytotoxic granule associated RNA binding protein Rox8 isoform
            X3 [Amblyomma americanum].
ACCESSION   XP_077499903
VERSION     XP_077499903.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643777.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..389
                     /product="TIA1 cytotoxic granule associated RNA binding
                     protein Rox8 isoform X3"
                     /calculated_mol_wt=42905
     Region          17..88
                     /region_name="RRM1_TIA1_like"
                     /note="RNA recognition motif 1 (RRM1) found in
                     granule-associated RNA binding proteins p40-TIA-1 and
                     TIAR; cd12352"
                     /db_xref="CDD:409788"
     Region          104..178
                     /region_name="RRM2_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins p40-TIA-1 and
                     TIAR; cd12353"
                     /db_xref="CDD:409789"
     Site            order(113,115,117,121,124,128..129)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:409789"
     Region          215..285
                     /region_name="RRM3_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins (p40-TIA-1 and
                     TIAR), and yeast nuclear and cytoplasmic polyadenylated
                     RNA-binding protein PUB1; cd12354"
                     /db_xref="CDD:409790"
     Region          <285..>386
                     /region_name="Med15"
                     /note="ARC105 or Med15 subunit of Mediator complex
                     non-fungal; pfam09606"
                     /db_xref="CDD:312941"
     CDS             1..389
                     /gene="Rox8"
                     /coded_by="XM_077643777.1:1..1170"
                     /db_xref="GeneID:144110701"
ORIGIN      
        1 mfsdfqdqgn dcqprtlyvg nldiavteel lvavfgqmgq vkgckiihep gndpycfvef
       61 sdhqsaasal lamnkrlcfg kemkvnwats pgntpkldts khhhifvgdl speiettqlr
      121 dafapfgdis dcrvvrdpqt lkskgygfvs fvkkadaena igtmngqwlg srairtnwat
      181 rkppanrtqa evdvttstkp ltfdevynqs sptnctvycg gitqglseel mqktfssyga
      241 iqeirvfkdk gyafirfgtk eaathaivat hnsdvngqtv kcswgkeatd pnnqqqpqnl
      301 atqysypyqq mgywypqagy pqvqgqfmqg vqypygqyyg qgfgsvgmqm awqglpgqpq
      361 vaaaqtpmaq glqqpgmiga ypmqqyqaq
//
LOCUS       XP_077499913             387 aa            linear   INV 25-NOV-2025
DEFINITION  TIA1 cytotoxic granule associated RNA binding protein Rox8 isoform
            X4 [Amblyomma americanum].
ACCESSION   XP_077499913
VERSION     XP_077499913.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643787.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..387
                     /product="TIA1 cytotoxic granule associated RNA binding
                     protein Rox8 isoform X4"
                     /calculated_mol_wt=42594
     Region          15..86
                     /region_name="RRM1_TIA1_like"
                     /note="RNA recognition motif 1 (RRM1) found in
                     granule-associated RNA binding proteins p40-TIA-1 and
                     TIAR; cd12352"
                     /db_xref="CDD:409788"
     Region          102..176
                     /region_name="RRM2_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins p40-TIA-1 and
                     TIAR; cd12353"
                     /db_xref="CDD:409789"
     Site            order(111,113,115,119,122,126..127)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:409789"
     Region          213..283
                     /region_name="RRM3_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins (p40-TIA-1 and
                     TIAR), and yeast nuclear and cytoplasmic polyadenylated
                     RNA-binding protein PUB1; cd12354"
                     /db_xref="CDD:409790"
     Region          <283..>384
                     /region_name="Med15"
                     /note="ARC105 or Med15 subunit of Mediator complex
                     non-fungal; pfam09606"
                     /db_xref="CDD:312941"
     CDS             1..387
                     /gene="Rox8"
                     /coded_by="XM_077643787.1:66..1229"
                     /db_xref="GeneID:144110701"
ORIGIN      
        1 mankdqgndc qprtlyvgnl diavteellv avfgqmgqvk gckiihepgn dpycfvefsd
       61 hqsaasalla mnkrlcfgke mkvnwatspg ntpkldtskh hhifvgdlsp eiettqlrda
      121 fapfgdisdc rvvrdpqtlk skgygfvsfv kkadaenaig tmngqwlgsr airtnwatrk
      181 ppanrtqaev dvttstkplt fdevynqssp tnctvycggi tqglseelmq ktfssygaiq
      241 eirvfkdkgy afirfgtkea athaivathn sdvngqtvkc swgkeatdpn nqqqpqnlat
      301 qysypyqqmg ywypqagypq vqgqfmqgvq ypygqyygqg fgsvgmqmaw qglpgqpqva
      361 aaqtpmaqgl qqpgmigayp mqqyqaq
//
LOCUS       XP_077499922            1163 aa            linear   INV 25-NOV-2025
DEFINITION  tankyrase [Amblyomma americanum].
ACCESSION   XP_077499922
VERSION     XP_077499922.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643796.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1163
                     /product="tankyrase"
                     /calculated_mol_wt=126304
     Region          7..343
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          58..86
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(61..62,65..67,69..70,74,77,86,88,90,94..95,98..100,
                     102..103,107,110,119,121,123,127..128,131..133,135..136,
                     140,143,152)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          88..119
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          121..152
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          177..>471
                     /region_name="PHA03095"
                     /note="ankyrin-like protein; Provisional"
                     /db_xref="CDD:222980"
     Region          211..239
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(214..215,218..220,222..223,227,230,239,241,243,
                     247..248,251..253,255..256,260,263,272,274,276,280..281,
                     284..286,288..289,293,296,305)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          241..272
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          274..305
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          362..395
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(363,367..368,371..372,376,378..379,383,386,395,397,
                     399,403..404,407..409,411..412,416,419,428,430,432,
                     436..437,440..442,444..445,449,452)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          397..428
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          430..460
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          493..781
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          527..555
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(530..531,534..536,538..539,543,546,555,557,559,
                     563..564,567..569,571..572,576,579,588,590,592,596..597,
                     600..602,604..605,609,612,621)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          557..588
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          590..621
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          647..698
                     /region_name="Ank_4"
                     /note="Ankyrin repeats (many copies); pfam13637"
                     /db_xref="CDD:372654"
     Site            order(677,679,683..684,687..689,691..692,696,699,708,710,
                     712,716..717,720..722,724..725,729,732,741,743,745,
                     749..750,753..755,757..758,762,765,774)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          677..708
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          710..741
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          743..774
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          876..934
                     /region_name="SAM_tankyrase1,2"
                     /note="SAM domain of tankyrase1,2 subfamily; cd09524"
                     /db_xref="CDD:188923"
     Region          947..1155
                     /region_name="tankyrase_like"
                     /note="Tankyrases interact with the telomere reverse
                     transcriptase complex (TERT). Tankyrase 1
                     poly-ADP-ribosylates Telomere Repeat Binding Factor 1
                     (TRF1) while Tankyrase 2 can poly-ADP-ribosylate itself or
                     TRF1. The tankyrases also contain multiple ankyrin...;
                     cd01438"
                     /db_xref="CDD:238718"
     CDS             1..1163
                     /gene="Tnks"
                     /coded_by="XM_077643796.1:27..3518"
                     /db_xref="GeneID:144110724"
ORIGIN      
        1 mmatrrahlp isldappags dagrelfegc rngdvtrvkk lvtphnvnsr dttgrkstpl
       61 hfaagfgrrd vvehllqnga nvhakddggl iplhnacsfg haevvqlllk hgadpnardn
      121 wnytplheaa ikgkvdvciv llqhgadasi rntdgkipld ladpstrsvl tgdyrkdell
      181 esarsgneek llslltsinv nchasdgrks tplhlaagyn rvrivqlllq hgadvhakdk
      241 gglvplhnac syghfevtem lvkhganvna mdlwqftplh eaasknraev cslllahgad
      301 pgllnchsks pvmvapsrel qdrmlyeykg halldaarqs dlarikkynt pdmanfkhpf
      361 tgdtalhmaa vspfpkrkqa velllrksan vnekneeylt plhlaadksh fdvvdvllkn
      421 gakvnaldsl gqtalhrcsr egnvqacrll lsggadpgiv svqgctaaql asdavqqllh
      481 epqrssadve fqlleaakag dlevvkklva chadivncrd vdgrqstplh faagynrvav
      541 veyllqhgad vhakdkgglv plhnacsygh yevadllvkh gasvnvsdlw kftplheata
      601 kgkydivkll lkhgadpskk nrdgntpldl vkdgdqdvad llkgdaalld aakkgnlarv
      661 mklvtpenin crdsqgrnst plhlaagynn levaelllen gadvnaqdkg gliplhnass
      721 yghldiaall ikyntvvnat drwgftplhe aaqkgrtqlc alllahgads tmknhegqtp
      781 ldiaaaedvr clltdampph alppsakaai pvqpqnassm lgagplsals lealeaeipa
      841 amsllstqra ghvregsaas eggapltelt ahnkefmqfl gsiglghlce lfekemitid
      901 ilaemgheel kqigvsaygh rhklmkgiek lltgraisst lmpttpgtvl vdltpddkey
      961 laveeelqat irehrdngya ggvfmrynii kiqkvrnrkl lerychrrke vaeenhnqsn
     1021 ermlfhgspf inaivqkgfd erhayiggmf gagiyfaens sksnqyvygt cggtgcpihk
     1081 drscyichrq mllcrvtlgk sflqfsamkm ahappghhsv igrpsvggls ypeyvvyrge
     1141 qaypeylisy qiekpqdhvv lgs
//
LOCUS       XP_077499934            1729 aa            linear   INV 25-NOV-2025
DEFINITION  cytoskeleton-associated protein 5-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077499934
VERSION     XP_077499934.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643808.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1729
                     /product="cytoskeleton-associated protein 5-like isoform
                     X1"
                     /calculated_mol_wt=188383
     Region          <720..778
                     /region_name="CLASP_N"
                     /note="CLASP N terminal; pfam12348"
                     /db_xref="CDD:432496"
     Region          906..936
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          947..973
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..1729
                     /gene="LOC144110734"
                     /coded_by="XM_077643808.1:240..5429"
                     /db_xref="GeneID:144110734"
ORIGIN      
        1 maddteylkl paedrcqhkl wkarlsgyed aaklfgsitd pkspefqkfv pfiknfvids
       61 navsqekgla avlsfvqnah lanrvcgdvl vglvskciaa pkqktrelaq eiifmyveie
      121 kqeavveelv kgldnknpkt vsgcvatlre clrlfgarvi svkplfkalp klledrdktv
      181 rdecklltie lyrwigdalk pllqslkpvq vteleaefak vekgtatper yirseqmrra
      241 qaaeadtdvv dggeegeecg gdtaapvday elleavdils klpknfyeqc eakkwqerke
      301 alekllelts npkleagdya dlvkvlkriv akdsnvivva laakclagla aglrqkfhpy
      361 anscisvyie kfkekkqnvv talrealdnt fqatdleail edvtaaldnk npqikgettl
      421 flarvfsklt pamlnkkllk alvtsllktl ndsdpavreg ssvslgtamk vvgervimpf
      481 lgdldnlkme kvkeccekae vvaapqkatr kpaaaaaaas pakgsaqpks eaqapcqkkp
      541 aaaaanpsvg vgvvkpaaaa pakatqkara tgpvkaqsss satskpvfse valsdeeaqg
      601 raaalfpees ltalgstiwk drlaaiekmk eiieamegsl pvqviaktlc rkpglrdtnf
      661 qvlklkletl vavlgrgpvs lcvadfllpd vvdkvgdikn gqsaaaalta laeatsldhv
      721 sqevlqlcfs qknpknqses llwlanaike fglkvplkav iesikkglaa snpavrtasi
      781 tlagvlylym gktlrslleg ekatlvqqld aelaklegqk ppapirglaa psggegtgga
      841 rdatddvqea avdmedlvpr tdisgqltes llselldkdw kvrqdalqkl aaivdqakfi
      901 cpklgdlpga lrarlldsnk nlaiqalnic qslgaalgph cgpyvrtlan gmftalgdsk
      961 nmvraaalsc lnewsshasl gtffenemlk dtlkaenpll rselfgwlae rlgpsdargv
     1021 pgaqlasclp hvyqsledrs aevrkkaqdl llpcmlhlgy esmaratskl kasskthvma
     1081 qldkvrpqlp akvppkgkgt ilregssqva pqqplattmp apnhadvmsp dqgavggsgr
     1141 grllksssrs kmlvkpgsss skssrkgeev dttpllsann lkeqriahet ahkllkwnfs
     1201 spreefhqql keqmgaanwa pslvtncfsa dfkmhiraid tllkflsssg gveatsanvd
     1261 lvlkwltlrf fdtnpsvllr aleylqalfp alcdtgyrmh dleassflpy lilkagdpkd
     1321 tvrkgvhdil rriykvfpgy kvfnylmqgl ssknarqrae cleelcflfe vlglpisept
     1381 pavllkevar hisdrdsavr naalncvvqa yfreekrvfk yigqlsdkdk slleerikrt
     1441 srtrqapmlp peesppiesq tpprpqsavf vsattvepaa epeevvnrrp etgaplgarp
     1501 rslgvtlssd lekmleqaas falpkvlslq evdvdaylse pdvqlpcrrl dksasppshe
     1561 vrrltvdahs pgtltsqats qeitvghegp aqntqafhqp cqenpsvntd ssscntpahn
     1621 pmndsnhdge sprkgdvqkl pkgyihwsqa ewaahlkaal eligikrhyq indypstqln
     1681 ntnifnepiq vhearytqmm qrsedllktg lqliselrgh nrrhensnc
//
LOCUS       XP_077499940            1630 aa            linear   INV 25-NOV-2025
DEFINITION  cytoskeleton-associated protein 5-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077499940
VERSION     XP_077499940.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643814.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1630
                     /product="cytoskeleton-associated protein 5-like isoform
                     X2"
                     /calculated_mol_wt=176866
     Region          <720..778
                     /region_name="CLASP_N"
                     /note="CLASP N terminal; pfam12348"
                     /db_xref="CDD:432496"
     Region          906..936
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          947..973
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..1630
                     /gene="LOC144110734"
                     /coded_by="XM_077643814.1:107..4999"
                     /db_xref="GeneID:144110734"
ORIGIN      
        1 maddteylkl paedrcqhkl wkarlsgyed aaklfgsitd pkspefqkfv pfiknfvids
       61 navsqekgla avlsfvqnah lanrvcgdvl vglvskciaa pkqktrelaq eiifmyveie
      121 kqeavveelv kgldnknpkt vsgcvatlre clrlfgarvi svkplfkalp klledrdktv
      181 rdecklltie lyrwigdalk pllqslkpvq vteleaefak vekgtatper yirseqmrra
      241 qaaeadtdvv dggeegeecg gdtaapvday elleavdils klpknfyeqc eakkwqerke
      301 alekllelts npkleagdya dlvkvlkriv akdsnvivva laakclagla aglrqkfhpy
      361 anscisvyie kfkekkqnvv talrealdnt fqatdleail edvtaaldnk npqikgettl
      421 flarvfsklt pamlnkkllk alvtsllktl ndsdpavreg ssvslgtamk vvgervimpf
      481 lgdldnlkme kvkeccekae vvaapqkatr kpaaaaaaas pakgsaqpks eaqapcqkkp
      541 aaaaanpsvg vgvvkpaaaa pakatqkara tgpvkaqsss satskpvfse valsdeeaqg
      601 raaalfpees ltalgstiwk drlaaiekmk eiieamegsl pvqviaktlc rkpglrdtnf
      661 qvlklkletl vavlgrgpvs lcvadfllpd vvdkvgdikn gqsaaaalta laeatsldhv
      721 sqevlqlcfs qknpknqses llwlanaike fglkvplkav iesikkglaa snpavrtasi
      781 tlagvlylym gktlrslleg ekatlvqqld aelaklegqk ppapirglaa psggegtgga
      841 rdatddvqea avdmedlvpr tdisgqltes llselldkdw kvrqdalqkl aaivdqakfi
      901 cpklgdlpga lrarlldsnk nlaiqalnic qslgaalgph cgpyvrtlan gmftalgdsk
      961 nmvraaalsc lnewsshasl gtffenemlk dtlkaenpll rselfgwlae rlgpsdargv
     1021 pgaqlasclp hvyqsledrs aevrkkaqdl llpcmlhlgy esmaratskl kasskthvma
     1081 qldkvrpqlp akvppkgkgt ilregssqva pqqplattmp apnhadvmsp dqgavggsgr
     1141 grllksssrs kmlvkpgsss skssrkgeev dttpllsann lkeqriahet ahkllkwnfs
     1201 spreefhqql keqmgaanwa pslvtncfsa dfkmhiraid tllkflsssg gveatsanvd
     1261 lvlkwltlrf fdtnpsvllr aleylqalfp alcdtgyrmh dleassflpy lilkagdpkd
     1321 tvrkgvhdil rriykvfpgy kvfnylmqgl ssknarqrae cleelcflfe vlglpisept
     1381 pavllkevar hisdrdsavr naalncvvqa yfreekrvfk yigqlsdkdk slleerikrt
     1441 srtrqapmlp peesppiesq tpprpqsavf vsattvepaa epeevvnrrp etgaplgarp
     1501 rslgvtlssd lekmleqaas falpkvlslq evdvdaylse pdvqlpcrrl dksasppshe
     1561 vrrltvdahs pgtltsqats qeitvghegp aqntqafhqp cqenpsvntd ssscntpahn
     1621 pmndsnhddi
//
LOCUS       XP_077499969             115 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144110764 [Amblyomma americanum].
ACCESSION   XP_077499969
VERSION     XP_077499969.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643843.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..115
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..115
                     /product="uncharacterized protein LOC144110764"
                     /calculated_mol_wt=12170
     CDS             1..115
                     /gene="LOC144110764"
                     /coded_by="XM_077643843.1:497..844"
                     /db_xref="GeneID:144110764"
ORIGIN      
        1 mfslesnnmw lvnsqhaaeg gleglpttph lavigssmgd ikkiivavds trslekamgl
       61 llgaffvfnv ayppdcpltm ellqrylgqs nptkgrgkgr acgiapklls llntl
//
LOCUS       XP_077499985             550 aa            linear   INV 25-NOV-2025
DEFINITION  HEAT repeat-containing protein 1-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077499985
VERSION     XP_077499985.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643859.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..550
                     /product="HEAT repeat-containing protein 1-like isoform
                     X1"
                     /calculated_mol_wt=59886
     CDS             1..550
                     /gene="LOC144110769"
                     /coded_by="XM_077643859.1:740..2392"
                     /db_xref="GeneID:144110769"
ORIGIN      
        1 mrlfnfvrff aqvfaqiiaf aflpvltarp pgivpgiwtp hqegeicarh pvatdtwepl
       61 atpplenwtk qaasaapssp tapfasaikq rhplsahcgl cgcatvrtan pltfvmqvfa
      121 qiiafaflpv ltarppgivp giwtphqege icarhpvatd tweplatppl enwtkqaasa
      181 apssptapfa saikqrhpls ahcglcgcat vrtanpltfv mqvskdlvkk lpeenqamll
      241 dtlvdlaldt ppsvqhsavs galrkvcffg sllveplqrt gvsqqspart vreakkmrlm
      301 daseeqdpae spqwkrvlil lemiqsrksl eevmllvgpl yallkrslel gmsphaeyir
      361 alvradgpag awpvgapprt cfpgqvpalh ypeevlhnvt svftykgtsl lwqvdscsfq
      421 tvmptvktvv pallqactge sksaeatesa valvtrvfvg pfpdipehww lslfaklatt
      481 lgagdhlwil aaqmaehqvt kmggtdvdev tfvrsveryr ektyrlrvea swegpfscis
      541 srpalentvp
//
LOCUS       XP_077499994             550 aa            linear   INV 25-NOV-2025
DEFINITION  HEAT repeat-containing protein 1-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077499994
VERSION     XP_077499994.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643868.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..550
                     /product="HEAT repeat-containing protein 1-like isoform
                     X1"
                     /calculated_mol_wt=59886
     CDS             1..550
                     /gene="LOC144110769"
                     /coded_by="XM_077643868.1:651..2303"
                     /db_xref="GeneID:144110769"
ORIGIN      
        1 mrlfnfvrff aqvfaqiiaf aflpvltarp pgivpgiwtp hqegeicarh pvatdtwepl
       61 atpplenwtk qaasaapssp tapfasaikq rhplsahcgl cgcatvrtan pltfvmqvfa
      121 qiiafaflpv ltarppgivp giwtphqege icarhpvatd tweplatppl enwtkqaasa
      181 apssptapfa saikqrhpls ahcglcgcat vrtanpltfv mqvskdlvkk lpeenqamll
      241 dtlvdlaldt ppsvqhsavs galrkvcffg sllveplqrt gvsqqspart vreakkmrlm
      301 daseeqdpae spqwkrvlil lemiqsrksl eevmllvgpl yallkrslel gmsphaeyir
      361 alvradgpag awpvgapprt cfpgqvpalh ypeevlhnvt svftykgtsl lwqvdscsfq
      421 tvmptvktvv pallqactge sksaeatesa valvtrvfvg pfpdipehww lslfaklatt
      481 lgagdhlwil aaqmaehqvt kmggtdvdev tfvrsveryr ektyrlrvea swegpfscis
      541 srpalentvp
//
LOCUS       XP_077500005             510 aa            linear   INV 25-NOV-2025
DEFINITION  HEAT repeat-containing protein 1-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077500005
VERSION     XP_077500005.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643879.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..510
                     /product="HEAT repeat-containing protein 1-like isoform
                     X2"
                     /calculated_mol_wt=55199
     CDS             1..510
                     /gene="LOC144110769"
                     /coded_by="XM_077643879.1:3482..5014"
                     /db_xref="GeneID:144110769"
ORIGIN      
        1 mrlfnfvrff aqvfaqiiaf aflpvltarp pgivpgiwtp hqegeicarh pvatdtwepl
       61 atpplenwtk qaasaapssp tapfasaikq rhplsahcgl cgcatvrtan pltfvmqvfa
      121 qiiafaflpv ltarppgivp giwtphqege icarhpvatd tweplatppl enwtkqaasa
      181 apssptapfa saikqrhpls ahcglcgcat vrtanpltfv mqvskdlvkk lpeenqamll
      241 dtlvdlaldt ppsvqhsavs galrkvcffg sllveplqrt gvsqqspart vreakkmrlm
      301 daseeqdpae spqwkrvlil lemiqsrksl eevmllvgpl yallkrslel gmsphaeyir
      361 alvradgpag awpvgapprt cfpgqvpalh ypeevlhnvt svftykgtsl lwqvdscsfq
      421 tvmptvktvv pallqactge sksaeatesa valvtrvfvg pfpdipehww lslfaklatt
      481 lgagdhlwil aaqmaehqvt kmggtdvdel
//
LOCUS       XP_077500012             340 aa            linear   INV 25-NOV-2025
DEFINITION  HEAT repeat-containing protein 1-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077500012
VERSION     XP_077500012.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643886.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..340
                     /product="HEAT repeat-containing protein 1-like isoform
                     X3"
                     /calculated_mol_wt=37618
     CDS             1..340
                     /gene="LOC144110769"
                     /coded_by="XM_077643886.1:3482..4504"
                     /db_xref="GeneID:144110769"
ORIGIN      
        1 mrlfnfvrff aqvskdlvkk lpeenqamll dtlvdlaldt ppsvqhsavs galrkvcffg
       61 sllveplqrt gvsqqspart vreakkmrlm daseeqdpae spqwkrvlil lemiqsrksl
      121 eevmllvgpl yallkrslel gmsphaeyir alvradgpag awpvgapprt cfpgqvpalh
      181 ypeevlhnvt svftykgtsl lwqvdscsfq tvmptvktvv pallqactge sksaeatesa
      241 valvtrvfvg pfpdipehww lslfaklatt lgagdhlwil aaqmaehqvt kmggtdvdev
      301 tfvrsveryr ektyrlrvea swegpfscis srpalentvp
//
LOCUS       XP_077500016             313 aa            linear   INV 25-NOV-2025
DEFINITION  HEAT repeat-containing protein 1-like isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077500016
VERSION     XP_077500016.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643890.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..313
                     /product="HEAT repeat-containing protein 1-like isoform
                     X4"
                     /calculated_mol_wt=34380
     CDS             1..313
                     /gene="LOC144110769"
                     /coded_by="XM_077643890.1:6287..7228"
                     /db_xref="GeneID:144110769"
ORIGIN      
        1 mlldtlvdla ldtppsvqhs avsgalrkvc ffgsllvepl qrtgvsqqsp artvreakkm
       61 rlmdaseeqd paespqwkrv lillemiqsr ksleevmllv gplyallkrs lelgmsphae
      121 yiralvradg pagawpvgap prtcfpgqvp alhypeevlh nvtsvftykg tsllwqvdsc
      181 sfqtvmptvk tvvpallqac tgesksaeat esavalvtrv fvgpfpdipe hwwlslfakl
      241 attlgagdhl wilaaqmaeh qvtkmggtdv devtfvrsve ryrektyrlr veaswegpfs
      301 cissrpalen tvp
//
LOCUS       XP_077500023             300 aa            linear   INV 25-NOV-2025
DEFINITION  HEAT repeat-containing protein 1-like isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077500023
VERSION     XP_077500023.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643897.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..300
                     /product="HEAT repeat-containing protein 1-like isoform
                     X5"
                     /calculated_mol_wt=32931
     CDS             1..300
                     /gene="LOC144110769"
                     /coded_by="XM_077643897.1:3482..4384"
                     /db_xref="GeneID:144110769"
ORIGIN      
        1 mrlfnfvrff aqvskdlvkk lpeenqamll dtlvdlaldt ppsvqhsavs galrkvcffg
       61 sllveplqrt gvsqqspart vreakkmrlm daseeqdpae spqwkrvlil lemiqsrksl
      121 eevmllvgpl yallkrslel gmsphaeyir alvradgpag awpvgapprt cfpgqvpalh
      181 ypeevlhnvt svftykgtsl lwqvdscsfq tvmptvktvv pallqactge sksaeatesa
      241 valvtrvfvg pfpdipehww lslfaklatt lgagdhlwil aaqmaehqvt kmggtdvdel
//
LOCUS       XP_077500031             300 aa            linear   INV 25-NOV-2025
DEFINITION  HEAT repeat-containing protein 1-like isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077500031
VERSION     XP_077500031.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643905.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..300
                     /product="HEAT repeat-containing protein 1-like isoform
                     X5"
                     /calculated_mol_wt=32931
     CDS             1..300
                     /gene="LOC144110769"
                     /coded_by="XM_077643905.1:740..1642"
                     /db_xref="GeneID:144110769"
ORIGIN      
        1 mrlfnfvrff aqvskdlvkk lpeenqamll dtlvdlaldt ppsvqhsavs galrkvcffg
       61 sllveplqrt gvsqqspart vreakkmrlm daseeqdpae spqwkrvlil lemiqsrksl
      121 eevmllvgpl yallkrslel gmsphaeyir alvradgpag awpvgapprt cfpgqvpalh
      181 ypeevlhnvt svftykgtsl lwqvdscsfq tvmptvktvv pallqactge sksaeatesa
      241 valvtrvfvg pfpdipehww lslfaklatt lgagdhlwil aaqmaehqvt kmggtdvdel
//
LOCUS       XP_077500035             300 aa            linear   INV 25-NOV-2025
DEFINITION  HEAT repeat-containing protein 1-like isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077500035
VERSION     XP_077500035.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643909.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..300
                     /product="HEAT repeat-containing protein 1-like isoform
                     X5"
                     /calculated_mol_wt=32931
     CDS             1..300
                     /gene="LOC144110769"
                     /coded_by="XM_077643909.1:651..1553"
                     /db_xref="GeneID:144110769"
ORIGIN      
        1 mrlfnfvrff aqvskdlvkk lpeenqamll dtlvdlaldt ppsvqhsavs galrkvcffg
       61 sllveplqrt gvsqqspart vreakkmrlm daseeqdpae spqwkrvlil lemiqsrksl
      121 eevmllvgpl yallkrslel gmsphaeyir alvradgpag awpvgapprt cfpgqvpalh
      181 ypeevlhnvt svftykgtsl lwqvdscsfq tvmptvktvv pallqactge sksaeatesa
      241 valvtrvfvg pfpdipehww lslfaklatt lgagdhlwil aaqmaehqvt kmggtdvdel
//
LOCUS       XP_077500040             273 aa            linear   INV 25-NOV-2025
DEFINITION  HEAT repeat-containing protein 1-like isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077500040
VERSION     XP_077500040.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643914.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..273
                     /product="HEAT repeat-containing protein 1-like isoform
                     X6"
                     /calculated_mol_wt=29694
     CDS             1..273
                     /gene="LOC144110769"
                     /coded_by="XM_077643914.1:5075..5896"
                     /db_xref="GeneID:144110769"
ORIGIN      
        1 mlldtlvdla ldtppsvqhs avsgalrkvc ffgsllvepl qrtgvsqqsp artvreakkm
       61 rlmdaseeqd paespqwkrv lillemiqsr ksleevmllv gplyallkrs lelgmsphae
      121 yiralvradg pagawpvgap prtcfpgqvp alhypeevlh nvtsvftykg tsllwqvdsc
      181 sfqtvmptvk tvvpallqac tgesksaeat esavalvtrv fvgpfpdipe hwwlslfakl
      241 attlgagdhl wilaaqmaeh qvtkmggtdv del
//
LOCUS       XP_077500051             980 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144110840 [Amblyomma americanum].
ACCESSION   XP_077500051
VERSION     XP_077500051.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643925.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..980
                     /product="uncharacterized protein LOC144110840"
                     /calculated_mol_wt=111427
     Region          16..576
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..980
                     /gene="LOC144110840"
                     /coded_by="XM_077643925.1:81..3023"
                     /db_xref="GeneID:144110840"
ORIGIN      
        1 mvkvrkvilt ptrrvymppq lvcksrllqh cdpdyalriv vrdddcklis fslgackddf
       61 lsavikppls sglkiggrrf mflgcstsql rshgvwfyah dnqgrtaeqi rdgigdlsti
      121 vgvpkfmarl gqafsqslgl ltvpsqntsv epdirhaapg rssdgshrey ifsdgigris
      181 hgllrkvhha leleegeepc avqiryggck gmllldptls gcrivfresm skfhsdhsdl
      241 yvlktskprv lylnrpmiti leqsgikaev flmlqnkild sfidsmmdph eaahvlrsyc
      301 alrlpykela gvgidltvep ffralvravn kkvlkelrtk arilvppnyg rtmfgvldet
      361 gtleygqvfv qysndmlqyk vndpatileg dvivtknpcm npgdirklea vnvpqlhhvr
      421 dcivfpqkge rphpdemags dldgdeysvl wyedlifnnn cnpmhyhsdp pkerkasigv
      481 qdmvdffcqy ikgdkiglia nahlvwadil dsginshrcr elarkcavnl dfakcgdlkg
      541 fqnsekppmy pdfmekldtk ntycsrkvlg qlyrnckkve lsteclevve eslpdprlll
      601 egreqflkea tsaykryakk irallksyri eteseafsga vsklskymke ndptdmamvl
      661 esqvehvvrr treeffseql deaheklkas awyqvtyelq sseggiqsfp wvvsdvlmri
      721 vvntssclpv pasrnsfcqr lgalllglph pgggdqdgtq hgdtqvltnl lrlmydwids
      781 sreflfvknt eelgvyksim reacfkvsrs isrdmpphkl vilclrfaca wclkifqggs
      841 dgeviskecr rryrlghlal itlnrlsmsg nlaylrrape gcpsteliri yinredeeff
      901 eilrryedii kkimmdwsgv ediqcdlktd rmdewflqlm vtgsrwaler lkeivvypsf
      961 revlllafer eknaiggtlq
//
LOCUS       XP_077500057             218 aa            linear   INV 25-NOV-2025
DEFINITION  HEAT repeat-containing protein 1-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077500057
VERSION     XP_077500057.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643931.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..218
                     /product="HEAT repeat-containing protein 1-like isoform
                     X1"
                     /calculated_mol_wt=23086
     CDS             1..218
                     /gene="LOC144110851"
                     /coded_by="XM_077643931.1:792..1448"
                     /db_xref="GeneID:144110851"
ORIGIN      
        1 msqpsaevgc sgrgflneev lhnmtsvfty kgasllwqvd ncsfqtvmpt vktvvpallq
       61 actgesksae aaesavasvt rvfvgpfpdi pghwwlslfa klattlgagd hlwilaaqma
      121 ehqvtkmggt gvdevtfvrs lqlttrqgar scctprpllm nswcfqqrlg grgttargst
      181 srsragallk lagklgaqal ngpgeprlrr pansaalr
//
LOCUS       XP_077500059             195 aa            linear   INV 25-NOV-2025
DEFINITION  HEAT repeat-containing protein 1-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077500059
VERSION     XP_077500059.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643933.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..195
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..195
                     /product="HEAT repeat-containing protein 1-like isoform
                     X2"
                     /calculated_mol_wt=20642
     CDS             1..195
                     /gene="LOC144110851"
                     /coded_by="XM_077643933.1:1201..1788"
                     /db_xref="GeneID:144110851"
ORIGIN      
        1 mtsvftykga sllwqvdncs fqtvmptvkt vvpallqact gesksaeaae savasvtrvf
       61 vgpfpdipgh wwlslfakla ttlgagdhlw ilaaqmaehq vtkmggtgvd evtfvrslql
      121 ttrqgarscc tprpllmnsw cfqqrlggrg ttargstsrs ragallklag klgaqalngp
      181 geprlrrpan saalr
//
LOCUS       XP_077500064             154 aa            linear   INV 25-NOV-2025
DEFINITION  HEAT repeat-containing protein 1-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077500064
VERSION     XP_077500064.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643938.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..154
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..154
                     /product="HEAT repeat-containing protein 1-like isoform
                     X3"
                     /calculated_mol_wt=16180
     CDS             1..154
                     /gene="LOC144110851"
                     /coded_by="XM_077643938.1:1102..1566"
                     /db_xref="GeneID:144110851"
ORIGIN      
        1 msqpsaevgc sgrgflneev lhnmtsvfty kgasllwqvd ncsfqtvmpt vktvvpallq
       61 actgesksae aaesavasvt rvfvgpfpdi pghwwlslfa klattlgagd hlwilaaqma
      121 ehqvtkmggt gvdelcsspq gkelglaalp dlfl
//
LOCUS       XP_077500104             161 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144110909 [Amblyomma americanum].
ACCESSION   XP_077500104
VERSION     XP_077500104.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643978.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..161
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..161
                     /product="uncharacterized protein LOC144110909"
                     /calculated_mol_wt=17311
     CDS             1..161
                     /gene="LOC144110909"
                     /coded_by="XM_077643978.1:1452..1937"
                     /db_xref="GeneID:144110909"
ORIGIN      
        1 mtgtgnckaq ghklasprna lsppsksrls hrdqqstang rssmasrsts rnrqnsskns
       61 srltwadrvs gnatrhpppp qssqkiretr nsgpcatrak fstgflseal rstcstpkas
      121 yilndfiikc cnasvsptnt mvashsrtwm knllgilskf g
//
LOCUS       XP_077500115             194 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144110918 [Amblyomma americanum].
ACCESSION   XP_077500115
VERSION     XP_077500115.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077643989.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..194
                     /product="uncharacterized protein LOC144110918"
                     /calculated_mol_wt=21166
     CDS             1..194
                     /gene="LOC144110918"
                     /coded_by="XM_077643989.1:440..1024"
                     /db_xref="GeneID:144110918"
ORIGIN      
        1 mtcalvfvcd dlcysldegq llttvlpflm ptepsevsla rlayastalq rlpelsnvta
       61 dkkitaalet edvdtiaell ladlgeaagk idqpltvvhs pslkhqvhfl gglwllhlda
      121 scravasssr qgaspslpke klqylalavs aqllqtaedi krilveqnlv pscchycrqy
      181 kgseswlsaw hrrh
//
LOCUS       XP_077500141             599 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144110940 [Amblyomma americanum].
ACCESSION   XP_077500141
VERSION     XP_077500141.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644015.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..599
                     /product="uncharacterized protein LOC144110940"
                     /calculated_mol_wt=67563
     Region          141..284
                     /region_name="Transposase_21"
                     /note="Transposase family tnp2; cl29371"
                     /db_xref="CDD:475188"
     CDS             1..599
                     /gene="LOC144110940"
                     /coded_by="XM_077644015.1:148..1947"
                     /db_xref="GeneID:144110940"
ORIGIN      
        1 mpsvqselne qayvsescae slssehtats cdssdetsst nsltaenvem gqpreespel
       61 cyrhlstaqe lasiaskhnl thacindlld fcrrrgivel pkdartimqt erkanlehgd
      121 sfvhfglaeg iqhavgqgpa penvelhani dglplhrssq talwpilcrv inikksepfm
      181 vsaycgagkp pclqeylrpf idevrnlscn grmvkgvhvn vclkavicda parsfvkait
      241 ghtgyhacer csqkghylen rmtfpdlhap qrtdssfrsq edrrhhtgvs pftslnvdmv
      301 kcfpteymhl vclgvmrrll rnwickghaa rlsradrsal ndklreaata fpkyfqrkpr
      361 gteeldrwka tefrtvllyl gpvllksilp delykhflyl hvsvrvlasp qhhrdlnqfa
      421 hdllryfvqe fgrlygqkql vynvhtlahl aeqclehgtv defsafpfes ylgkvkkrlr
      481 ttnkplaqls rrmsevract plstgqvggd vkvgdcflvd ncavvivdll ggnakagilp
      541 ngreffrvpi dssrmdvrry salgqetkiw pisyiknkvr ciklqykrgh vvfpllhlq
//
LOCUS       XP_077500151             323 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144110950 [Amblyomma americanum].
ACCESSION   XP_077500151
VERSION     XP_077500151.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644025.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..323
                     /product="uncharacterized protein LOC144110950"
                     /calculated_mol_wt=36319
     Region          <7..277
                     /region_name="COG3335"
                     /note="Transposase [Mobilome: prophages, transposons]"
                     /db_xref="CDD:442564"
     CDS             1..323
                     /gene="LOC144110950"
                     /coded_by="XM_077644025.1:1238..2209"
                     /db_xref="GeneID:144110950"
ORIGIN      
        1 mgfkfekrkr tlalkersdi vawrrrylra ikafrsqgrc ivyldetwvn aghtrqhvwk
       61 dstvkssqda flrglttglp apsgkggrvi lvhagssltg fvsgaadffr akkgasahyh
      121 semdgkyfee wfsskllpni pnssiivmdn apyhsvalek ppttstrkie iqawltrkgi
      181 ywstdmvrae lldlckitts ghtvyaidtl aakhghevlr lppyhcefnp ieqvwsmvkg
      241 fvakrnkaft laeveellpq alahvskedw qkfcahaeri edeawerdii fddevdpvvf
      301 aigsssssse essselsgie ele
//
LOCUS       XP_077500167             309 aa            linear   INV 25-NOV-2025
DEFINITION  guanylate kinase isoform X1 [Amblyomma americanum].
ACCESSION   XP_077500167
VERSION     XP_077500167.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644041.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..309
                     /product="guanylate kinase isoform X1"
                     /calculated_mol_wt=34308
     Region          <22..>127
                     /region_name="T7SS_EccC_b"
                     /note="type VII secretion protein EccCb; TIGR03925"
                     /db_xref="CDD:274859"
     Region          106..283
                     /region_name="guanyl_kin"
                     /note="guanylate kinase; TIGR03263"
                     /db_xref="CDD:213788"
     Site            order(112,118,135,142,145,154,177,182)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:238026"
     Site            order(112,118)
                     /site_type="other"
                     /note="G-X2-G-X-G-K"
                     /db_xref="CDD:238026"
     CDS             1..309
                     /gene="LOC144110966"
                     /coded_by="XM_077644041.1:17..946"
                     /db_xref="GeneID:144110966"
ORIGIN      
        1 mfvkvsklln vaaaprrsgs staaprvsdh agtvdtaacs ssstrvrmqd ehtfylrkad
       61 gpiavqapnl eplydrgptr vpppamlaqa kresglstli hmsalrplvi cgpsgsgkst
      121 llrkllkdfg dyfalcvsht trkprpgevn gkdyhfisrd emeqaieage fieytefsgn
      181 lygtskksvr dvqdqgrici ldieiegvkn ikntdlnpry ifvkppsmka leerlrgrgt
      241 eseeslrrrl akaseeiayg enqgnfdlll vndnlkgayn rlrdylikey svgfsiiqev
      301 eelqkkpki
//
LOCUS       XP_077500173             300 aa            linear   INV 25-NOV-2025
DEFINITION  guanylate kinase isoform X2 [Amblyomma americanum].
ACCESSION   XP_077500173
VERSION     XP_077500173.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644047.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..300
                     /product="guanylate kinase isoform X2"
                     /calculated_mol_wt=33255
     Region          <22..>128
                     /region_name="T7SS_EccC_b"
                     /note="type VII secretion protein EccCb; TIGR03925"
                     /db_xref="CDD:274859"
     Region          107..284
                     /region_name="guanyl_kin"
                     /note="guanylate kinase; TIGR03263"
                     /db_xref="CDD:213788"
     Site            order(113,119,136,143,146,155,178,183)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:238026"
     Site            order(113,119)
                     /site_type="other"
                     /note="G-X2-G-X-G-K"
                     /db_xref="CDD:238026"
     CDS             1..300
                     /gene="LOC144110966"
                     /coded_by="XM_077644047.1:22..924"
                     /db_xref="GeneID:144110966"
ORIGIN      
        1 mfvkvsklln vaaaprrsgs staaprvsdh agtvdtaacs ssstrvrmqd ehtfylrkad
       61 gpiavqapnl eplydrgptr vpppamlaqa kretgglstl ihmsalrplv icgpsgsgks
      121 tllrkllkdf gdyfalcvsh ttrkprpgev ngkdyhfisr demeqaieag efieytefsg
      181 nlygtskksv rdvqdqgric ildieiegvk nikntdlnpr yifvkppsmk aleerlrgrg
      241 teseeslrrr lakaseeiay genqgnfdll lvndnlkgay nrlrdylike veelqkkpki
//
LOCUS       XP_077500181             310 aa            linear   INV 25-NOV-2025
DEFINITION  guanylate kinase isoform X3 [Amblyomma americanum].
ACCESSION   XP_077500181
VERSION     XP_077500181.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644055.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..310
                     /product="guanylate kinase isoform X3"
                     /calculated_mol_wt=34379
     Region          <22..>128
                     /region_name="T7SS_EccC_b"
                     /note="type VII secretion protein EccCb; TIGR03925"
                     /db_xref="CDD:274859"
     Region          107..284
                     /region_name="guanyl_kin"
                     /note="guanylate kinase; TIGR03263"
                     /db_xref="CDD:213788"
     Site            order(113,119,136,143,146,155,178,183)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:238026"
     Site            order(113,119)
                     /site_type="other"
                     /note="G-X2-G-X-G-K"
                     /db_xref="CDD:238026"
     CDS             1..310
                     /gene="LOC144110966"
                     /coded_by="XM_077644055.1:16..948"
                     /db_xref="GeneID:144110966"
ORIGIN      
        1 mfvkvsklln vaaaprrsgs staaprvsdh agtvdtaacs ssstrvrmqd ehtfylrkad
       61 gpiavqapnl eplydrgptr vpppamlaqa kretgglstl ihmsalrplv icgpsgsgks
      121 tllrkllkdf gdyfalcvsh ttrkprpgev ngkdyhfisr demeqaieag efieytefsg
      181 nlygtskksv rdvqdqgric ildieiegvk nikntdlnpr yifvkppsmk aleerlrgrg
      241 teseeslrrr lakaseeiay genqgnfdll lvndnlkgay nrlrdylike ysvgfsiiqe
      301 veelqkkpki
//
LOCUS       XP_077500188             242 aa            linear   INV 25-NOV-2025
DEFINITION  guanylate kinase isoform X4 [Amblyomma americanum].
ACCESSION   XP_077500188
VERSION     XP_077500188.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644062.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..242
                     /product="guanylate kinase isoform X4"
                     /calculated_mol_wt=27381
     Region          39..216
                     /region_name="guanyl_kin"
                     /note="guanylate kinase; TIGR03263"
                     /db_xref="CDD:213788"
     Site            order(45,51,68,75,78,87,110,115)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:238026"
     Site            order(45,51)
                     /site_type="other"
                     /note="G-X2-G-X-G-K"
                     /db_xref="CDD:238026"
     CDS             1..242
                     /gene="LOC144110966"
                     /coded_by="XM_077644062.1:102..830"
                     /db_xref="GeneID:144110966"
ORIGIN      
        1 maaiffrtiq gwietkgtrs prrvrtggls tlihmsalrp lvicgpsgsg kstllrkllk
       61 dfgdyfalcv shttrkprpg evngkdyhfi srdemeqaie agefieytef sgnlygtskk
      121 svrdvqdqgr icildieieg vknikntdln pryifvkpps mkaleerlrg rgteseeslr
      181 rrlakaseei aygenqgnfd lllvndnlkg aynrlrdyli keysvgfsii qeveelqkkp
      241 ki
//
LOCUS       XP_077500197             225 aa            linear   INV 25-NOV-2025
DEFINITION  guanylate kinase isoform X5 [Amblyomma americanum].
ACCESSION   XP_077500197
VERSION     XP_077500197.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644071.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..225
                     /product="guanylate kinase isoform X5"
                     /calculated_mol_wt=25355
     Region          22..199
                     /region_name="guanyl_kin"
                     /note="guanylate kinase; TIGR03263"
                     /db_xref="CDD:213788"
     Site            order(28,34,51,58,61,70,93,98)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:238026"
     Site            order(28,34)
                     /site_type="other"
                     /note="G-X2-G-X-G-K"
                     /db_xref="CDD:238026"
     CDS             1..225
                     /gene="LOC144110966"
                     /coded_by="XM_077644071.1:143..820"
                     /db_xref="GeneID:144110966"
ORIGIN      
        1 mysmvnhtag glstlihmsa lrplvicgps gsgkstllrk llkdfgdyfa lcvshttrkp
       61 rpgevngkdy hfisrdemeq aieagefiey tefsgnlygt skksvrdvqd qgricildie
      121 iegvkniknt dlnpryifvk ppsmkaleer lrgrgtesee slrrrlakas eeiaygenqg
      181 nfdlllvndn lkgaynrlrd ylikeysvgf siiqeveelq kkpki
//
LOCUS       XP_077500206             221 aa            linear   INV 25-NOV-2025
DEFINITION  guanylate kinase isoform X6 [Amblyomma americanum].
ACCESSION   XP_077500206
VERSION     XP_077500206.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644080.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..221
                     /product="guanylate kinase isoform X6"
                     /calculated_mol_wt=24938
     Region          18..195
                     /region_name="guanyl_kin"
                     /note="guanylate kinase; TIGR03263"
                     /db_xref="CDD:213788"
     Site            order(24,30,47,54,57,66,89,94)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:238026"
     Site            order(24,30)
                     /site_type="other"
                     /note="G-X2-G-X-G-K"
                     /db_xref="CDD:238026"
     CDS             1..221
                     /gene="LOC144110966"
                     /coded_by="XM_077644080.1:360..1025"
                     /db_xref="GeneID:144110966"
ORIGIN      
        1 mrmkagglst lihmsalrpl vicgpsgsgk stllrkllkd fgdyfalcvs httrkprpge
       61 vngkdyhfis rdemeqaiea gefieytefs gnlygtskks vrdvqdqgri cildieiegv
      121 knikntdlnp ryifvkppsm kaleerlrgr gteseeslrr rlakaseeia ygenqgnfdl
      181 llvndnlkga ynrlrdylik eysvgfsiiq eveelqkkpk i
//
LOCUS       XP_077500212             220 aa            linear   INV 25-NOV-2025
DEFINITION  guanylate kinase isoform X7 [Amblyomma americanum].
ACCESSION   XP_077500212
VERSION     XP_077500212.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644086.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..220
                     /product="guanylate kinase isoform X7"
                     /calculated_mol_wt=24867
     Region          17..194
                     /region_name="guanyl_kin"
                     /note="guanylate kinase; TIGR03263"
                     /db_xref="CDD:213788"
     Site            order(23,29,46,53,56,65,88,93)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:238026"
     Site            order(23,29)
                     /site_type="other"
                     /note="G-X2-G-X-G-K"
                     /db_xref="CDD:238026"
     CDS             1..220
                     /gene="LOC144110966"
                     /coded_by="XM_077644086.1:139..801"
                     /db_xref="GeneID:144110966"
ORIGIN      
        1 mrmkgglstl ihmsalrplv icgpsgsgks tllrkllkdf gdyfalcvsh ttrkprpgev
       61 ngkdyhfisr demeqaieag efieytefsg nlygtskksv rdvqdqgric ildieiegvk
      121 nikntdlnpr yifvkppsmk aleerlrgrg teseeslrrr lakaseeiay genqgnfdll
      181 lvndnlkgay nrlrdylike ysvgfsiiqe veelqkkpki
//
LOCUS       XP_077500219             211 aa            linear   INV 25-NOV-2025
DEFINITION  guanylate kinase isoform X8 [Amblyomma americanum].
ACCESSION   XP_077500219
VERSION     XP_077500219.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644093.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..211
                     /product="guanylate kinase isoform X8"
                     /calculated_mol_wt=23813
     Region          18..195
                     /region_name="guanyl_kin"
                     /note="guanylate kinase; TIGR03263"
                     /db_xref="CDD:213788"
     Site            order(24,30,47,54,57,66,89,94)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:238026"
     Site            order(24,30)
                     /site_type="other"
                     /note="G-X2-G-X-G-K"
                     /db_xref="CDD:238026"
     CDS             1..211
                     /gene="LOC144110966"
                     /coded_by="XM_077644093.1:146..781"
                     /db_xref="GeneID:144110966"
ORIGIN      
        1 mrmkagglst lihmsalrpl vicgpsgsgk stllrkllkd fgdyfalcvs httrkprpge
       61 vngkdyhfis rdemeqaiea gefieytefs gnlygtskks vrdvqdqgri cildieiegv
      121 knikntdlnp ryifvkppsm kaleerlrgr gteseeslrr rlakaseeia ygenqgnfdl
      181 llvndnlkga ynrlrdylik eveelqkkpk i
//
LOCUS       XP_077500230             236 aa            linear   INV 25-NOV-2025
DEFINITION  bax Inhibitor-1 [Amblyomma americanum].
ACCESSION   XP_077500230
VERSION     XP_077500230.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644104.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..236
                     /product="bax Inhibitor-1"
                     /calculated_mol_wt=26147
     Region          16..229
                     /region_name="BI-1"
                     /note="BAX inhibitor (BI)-1; cd10430"
                     /db_xref="CDD:198412"
     CDS             1..236
                     /gene="BI-1"
                     /coded_by="XM_077644104.1:111..821"
                     /db_xref="GeneID:144111015"
ORIGIN      
        1 measfnmnaf mnsfsnrldk pvrdhlknvy sclaistlat aaggyvhlft dilqgnfltt
       61 llslgllval fgipdngknq hvrlailvgf afttglgmgp lldvvvsvdp siiataflat
      121 cavftcfsls alytdhcrwi yvggtlmsil svmslftlan lflgsyllfq anlylgvavf
      181 cgfvlydtql iiekrkrger dyirhsvdlf ldfisifkrl liiltqkess rrekrn
//
LOCUS       XP_077500249             360 aa            linear   INV 25-NOV-2025
DEFINITION  TM2 and DnaJ domain-containing protein wurst [Amblyomma
            americanum].
ACCESSION   XP_077500249
VERSION     XP_077500249.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644123.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..360
                     /product="TM2 and DnaJ domain-containing protein wurst"
                     /calculated_mol_wt=41536
     Region          <20..51
                     /region_name="TM2"
                     /note="TM2 domain; pfam05154"
                     /db_xref="CDD:428337"
     Region          283..343
                     /region_name="DnaJ"
                     /note="DnaJ domain; pfam00226"
                     /db_xref="CDD:395170"
     Site            order(308..310,322,325..326,329..330)
                     /site_type="other"
                     /note="HSP70 interaction site [polypeptide binding]"
                     /db_xref="CDD:99751"
     CDS             1..360
                     /gene="wus"
                     /coded_by="XM_077644123.1:237..1319"
                     /db_xref="GeneID:144111030"
ORIGIN      
        1 maksllvtyl lwltlgwlga hhfylrrdrq afvwwmtlgg yfglgwvrdi wkipdyvkya
       61 nsdpeavekl kenirrhakp psstvrsigq mivadalgym ilyalpvdhi ppkymplaml
      121 pvplavavgv hlvgnigeqt gnfkmpllga ylsyplyfws thsvfwtsll ssycfnnyak
      181 swrlkprpak plyqrlavfv tcgllytslw aswlyfncsm tdndgtevkc rdsiqhffgs
      241 plwqdfksvv mamyhqlrhn gwgelwrllm ealdpqgean alkvlgvqss atqeaitaay
      301 rklarqwhpd rfkepdkkpq aqekfieiqk ayevlsnlks rrlkknaqer edpddsrsef
//
LOCUS       XP_077500259            1796 aa            linear   INV 25-NOV-2025
DEFINITION  disco-interacting protein 2 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077500259
VERSION     XP_077500259.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644133.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1796
                     /product="disco-interacting protein 2 isoform X1"
                     /calculated_mol_wt=195810
     Region          <251..338
                     /region_name="DMAP_binding"
                     /note="DMAP1-binding Domain; pfam06464"
                     /db_xref="CDD:368923"
     Region          563..1138
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(723,726..731,733..734)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(726,846..847,905..910,1003,1015,1018,1029,1118)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(726,767..768,818,820..821,824,846..847,865..870,
                     1003,1015,1018,1026..1029,1099)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(767,820..821,824,846,1026..1028,1086,1099)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Region          1216..1792
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1364,1367..1372,1374..1375)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1367,1493..1494,1548..1553,1644,1679,1682,1693,1772)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1367,1408..1409,1459,1461..1462,1465,1493..1494,
                     1511..1516,1644,1679,1682,1690..1693,1753)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1408,1461..1462,1465,1493,1690..1692,1740,1753)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     CDS             1..1796
                     /gene="DIP2"
                     /coded_by="XM_077644133.1:16..5406"
                     /db_xref="GeneID:144111035"
ORIGIN      
        1 msgyrialsr evadvvlvlp pfvfrkqgvl hpsgpadykv wpvpapragg dvfplsasvg
       61 dggisrsngr ywtggpvlsl gaasffvrth rwrraqalpv aaaarvvnrr ssgapppgrp
      121 plafplgaew raaggrrrwf saraaaarfa aeriddgqig vrllsppwap rllssgrahv
      181 pswatwsqra amqrprkpsl feapiwkslp yslwaalldd vdeessdded dsesslfssr
      241 vssegclsdt esditqkgye kkrarllapy lpshptalpt gsaspstrak rraqrrltrn
      301 esryhsevrq eavqqalaam qnkpkpslpm pskrtsvmtt spsrerhpsd sssdddsvln
      361 egstpeqerl mrplptppqs dtsgasspre tpprialrcs ngrgsdrptv dgvshladvm
      421 qnltpseytd lnpqpdvtnt ginanhtpte hanrynqahm ssvrvtqhsa tgleygtgkw
      481 kvsakiqqll ntlkrpkrrp lpefyvddev dmdsnpsdpn apqpegatmt ptvgenlevp
      541 sglpqsleaa ikrygsgtfk fpaatvldts gkigaplpyg kllsrswkia fnllnklgqk
      601 gegqalkpgd rvalvypnnd pvafmcsfyg clmagivpvp ievpltrrda gsqqigfllg
      661 scgvsyalts evcykglpkt atgeihtfkg wpklhwfvte hlskppkdws pptrltdeta
      721 ayieytvdrd gamkgvavsr salvnhsral taacnytege vmvcvldykr emglwhgvla
      781 svfngmhvif ipyslmkvnp aswmlmitkf kanvavcksr dlhwgllatk dhkdvnlssl
      841 rlllvadgsn pwslsscdqf isvfhsrglh pdavcpcaas pealtvavrr pgrvgagasg
      901 rgvlsmaals ygvirvdmen sltsltlqdc ghilpgavaa vvkindaphl cktdevgeic
      961 vsastcgysy wglqglsnni fkmqplhpdg rpvtdqafvr sgllgfvgpg glvfvcgtrd
     1021 glmqvsmrkh ntddliatvl avepmkfiyr griavfsikv lrdericvva eqrpdcteee
     1081 sfqwmsrvlq avdsihqvgi yclalvppny lpktplggih lpetkrrfle gslhpanvlm
     1141 cphtcvtnlp kprevhpevg pasvmvgnmv qgvrlamaqg rdigiideds dtarkyqfis
     1201 eilrwrattt adhavftlln akgntasslt csqlhkraer vgclllekgk lntgdhvali
     1261 yppgidlica fygclyvgvv pvtirpphpq nlqttlptvr mivdvsksvi vlstapvtkl
     1321 lrskeasnvv dvkswrmile tddlpkrkls siyraptpem iayldfsvst tgmlagikms
     1381 haaatslcrs mklacelyps rhialcldpy cglgfvlwcl nsvysghhsi lippsevevn
     1441 paiwlttvsq ykvrdtfcsy gvmelctkgl gssiaqlkqr nvnlscvrtc vvvaeerpri
     1501 alttsfsklf sglglsprav stsfgcrvnv aiclqgassp dpsqvyvdmr alrndrvtlv
     1561 ergaphslcl lesgkllpgl klvianpdtr gqcgdshlge iwvqsphnas gyftvygdes
     1621 qhndhfnakl itgdtgsvya rtgylgflrr teavssdnet dseaasnvdg pelhdavfvv
     1681 galdetmmlr gmryhpidve nsvlrchkki secavftwtn llvvvveldg nesealdlvp
     1741 lvtsvvleeh qlvvgvvvvv dpgvvpinsr gekqrmhlrd gfladqldpi yvaynm
//
LOCUS       XP_077500267            1795 aa            linear   INV 25-NOV-2025
DEFINITION  disco-interacting protein 2 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077500267
VERSION     XP_077500267.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644141.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1795
                     /product="disco-interacting protein 2 isoform X2"
                     /calculated_mol_wt=195708
     Region          <251..338
                     /region_name="DMAP_binding"
                     /note="DMAP1-binding Domain; pfam06464"
                     /db_xref="CDD:368923"
     Region          562..1137
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(722,725..730,732..733)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(725,845..846,904..909,1002,1014,1017,1028,1117)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(725,766..767,817,819..820,823,845..846,864..869,
                     1002,1014,1017,1025..1028,1098)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(766,819..820,823,845,1025..1027,1085,1098)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Region          1215..1791
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1363,1366..1371,1373..1374)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1366,1492..1493,1547..1552,1643,1678,1681,1692,1771)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1366,1407..1408,1458,1460..1461,1464,1492..1493,
                     1510..1515,1643,1678,1681,1689..1692,1752)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1407,1460..1461,1464,1492,1689..1691,1739,1752)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     CDS             1..1795
                     /gene="DIP2"
                     /coded_by="XM_077644141.1:16..5403"
                     /db_xref="GeneID:144111035"
ORIGIN      
        1 msgyrialsr evadvvlvlp pfvfrkqgvl hpsgpadykv wpvpapragg dvfplsasvg
       61 dggisrsngr ywtggpvlsl gaasffvrth rwrraqalpv aaaarvvnrr ssgapppgrp
      121 plafplgaew raaggrrrwf saraaaarfa aeriddgqig vrllsppwap rllssgrahv
      181 pswatwsqra amqrprkpsl feapiwkslp yslwaalldd vdeessdded dsesslfssr
      241 vssegclsdt esditqkgye kkrarllapy lpshptalpt gsaspstrak rraqrrltrn
      301 esryhsevrq eavqqalaam qnkpkpslpm pskrtsvmtt spsrerhpsd sssdddsvln
      361 egstpeqerl mrplptppqs dtsgasspre tpprialrcs ngrgsdrptv dgvshladvm
      421 qnltpseynl npqpdvtntg inanhtpteh anrynqahms svrvtqhsat gleygtgkwk
      481 vsakiqqlln tlkrpkrrpl pefyvddevd mdsnpsdpna pqpegatmtp tvgenlevps
      541 glpqsleaai krygsgtfkf paatvldtsg kigaplpygk llsrswkiaf nllnklgqkg
      601 egqalkpgdr valvypnndp vafmcsfygc lmagivpvpi evpltrrdag sqqigfllgs
      661 cgvsyaltse vcykglpkta tgeihtfkgw pklhwfvteh lskppkdwsp ptrltdetaa
      721 yieytvdrdg amkgvavsrs alvnhsralt aacnytegev mvcvldykre mglwhgvlas
      781 vfngmhvifi pyslmkvnpa swmlmitkfk anvavcksrd lhwgllatkd hkdvnlsslr
      841 lllvadgsnp wslsscdqfi svfhsrglhp davcpcaasp ealtvavrrp grvgagasgr
      901 gvlsmaalsy gvirvdmens ltsltlqdcg hilpgavaav vkindaphlc ktdevgeicv
      961 sastcgysyw glqglsnnif kmqplhpdgr pvtdqafvrs gllgfvgpgg lvfvcgtrdg
     1021 lmqvsmrkhn tddliatvla vepmkfiyrg riavfsikvl rdericvvae qrpdcteees
     1081 fqwmsrvlqa vdsihqvgiy clalvppnyl pktplggihl petkrrfleg slhpanvlmc
     1141 phtcvtnlpk prevhpevgp asvmvgnmvq gvrlamaqgr digiidedsd tarkyqfise
     1201 ilrwrattta dhavftllna kgntassltc sqlhkraerv gclllekgkl ntgdhvaliy
     1261 ppgidlicaf ygclyvgvvp vtirpphpqn lqttlptvrm ivdvsksviv lstapvtkll
     1321 rskeasnvvd vkswrmilet ddlpkrklss iyraptpemi ayldfsvstt gmlagikmsh
     1381 aaatslcrsm klacelypsr hialcldpyc glgfvlwcln svysghhsil ippsevevnp
     1441 aiwlttvsqy kvrdtfcsyg vmelctkglg ssiaqlkqrn vnlscvrtcv vvaeerpria
     1501 lttsfsklfs glglspravs tsfgcrvnva iclqgasspd psqvyvdmra lrndrvtlve
     1561 rgaphslcll esgkllpglk lvianpdtrg qcgdshlgei wvqsphnasg yftvygdesq
     1621 hndhfnakli tgdtgsvyar tgylgflrrt eavssdnetd seaasnvdgp elhdavfvvg
     1681 aldetmmlrg mryhpidven svlrchkkis ecavftwtnl lvvvveldgn esealdlvpl
     1741 vtsvvleehq lvvgvvvvvd pgvvpinsrg ekqrmhlrdg fladqldpiy vaynm
//
LOCUS       XP_077500277            1795 aa            linear   INV 25-NOV-2025
DEFINITION  disco-interacting protein 2 isoform X3 [Amblyomma americanum].
ACCESSION   XP_077500277
VERSION     XP_077500277.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644151.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1795
                     /product="disco-interacting protein 2 isoform X3"
                     /calculated_mol_wt=195681
     Region          <251..338
                     /region_name="DMAP_binding"
                     /note="DMAP1-binding Domain; pfam06464"
                     /db_xref="CDD:368923"
     Region          563..1138
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(723,726..731,733..734)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(726,846..847,905..910,1003,1015,1018,1029,1118)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(726,767..768,818,820..821,824,846..847,865..870,
                     1003,1015,1018,1026..1029,1099)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(767,820..821,824,846,1026..1028,1086,1099)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Region          1215..1791
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1363,1366..1371,1373..1374)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1366,1492..1493,1547..1552,1643,1678,1681,1692,1771)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1366,1407..1408,1458,1460..1461,1464,1492..1493,
                     1510..1515,1643,1678,1681,1689..1692,1752)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1407,1460..1461,1464,1492,1689..1691,1739,1752)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     CDS             1..1795
                     /gene="DIP2"
                     /coded_by="XM_077644151.1:16..5403"
                     /db_xref="GeneID:144111035"
ORIGIN      
        1 msgyrialsr evadvvlvlp pfvfrkqgvl hpsgpadykv wpvpapragg dvfplsasvg
       61 dggisrsngr ywtggpvlsl gaasffvrth rwrraqalpv aaaarvvnrr ssgapppgrp
      121 plafplgaew raaggrrrwf saraaaarfa aeriddgqig vrllsppwap rllssgrahv
      181 pswatwsqra amqrprkpsl feapiwkslp yslwaalldd vdeessdded dsesslfssr
      241 vssegclsdt esditqkgye kkrarllapy lpshptalpt gsaspstrak rraqrrltrn
      301 esryhsevrq eavqqalaam qnkpkpslpm pskrtsvmtt spsrerhpsd sssdddsvln
      361 egstpeqerl mrplptppqs dtsgasspre tpprialrcs ngrgsdrptv dgvshladvm
      421 qnltpseytd lnpqpdvtnt ginanhtpte hanrynqahm ssvrvtqhsa tgleygtgkw
      481 kvsakiqqll ntlkrpkrrp lpefyvddev dmdsnpsdpn apqpegatmt ptvgenlevp
      541 sglpqsleaa ikrygsgtfk fpaatvldts gkigaplpyg kllsrswkia fnllnklgqk
      601 gegqalkpgd rvalvypnnd pvafmcsfyg clmagivpvp ievpltrrda gsqqigfllg
      661 scgvsyalts evcykglpkt atgeihtfkg wpklhwfvte hlskppkdws pptrltdeta
      721 ayieytvdrd gamkgvavsr salvnhsral taacnytege vmvcvldykr emglwhgvla
      781 svfngmhvif ipyslmkvnp aswmlmitkf kanvavcksr dlhwgllatk dhkdvnlssl
      841 rlllvadgsn pwslsscdqf isvfhsrglh pdavcpcaas pealtvavrr pgrvgagasg
      901 rgvlsmaals ygvirvdmen sltsltlqdc ghilpgavaa vvkindaphl cktdevgeic
      961 vsastcgysy wglqglsnni fkmqplhpdg rpvtdqafvr sgllgfvgpg glvfvcgtrd
     1021 glmqvsmrkh ntddliatvl avepmkfiyr griavfsikv lrdericvva eqrpdcteee
     1081 sfqwmsrvlq avdsihqvgi yclalvppny lpktplggih lpetkrrfle gslhpanvlm
     1141 cphtcvtnlp kprevhpvgp asvmvgnmvq gvrlamaqgr digiidedsd tarkyqfise
     1201 ilrwrattta dhavftllna kgntassltc sqlhkraerv gclllekgkl ntgdhvaliy
     1261 ppgidlicaf ygclyvgvvp vtirpphpqn lqttlptvrm ivdvsksviv lstapvtkll
     1321 rskeasnvvd vkswrmilet ddlpkrklss iyraptpemi ayldfsvstt gmlagikmsh
     1381 aaatslcrsm klacelypsr hialcldpyc glgfvlwcln svysghhsil ippsevevnp
     1441 aiwlttvsqy kvrdtfcsyg vmelctkglg ssiaqlkqrn vnlscvrtcv vvaeerpria
     1501 lttsfsklfs glglspravs tsfgcrvnva iclqgasspd psqvyvdmra lrndrvtlve
     1561 rgaphslcll esgkllpglk lvianpdtrg qcgdshlgei wvqsphnasg yftvygdesq
     1621 hndhfnakli tgdtgsvyar tgylgflrrt eavssdnetd seaasnvdgp elhdavfvvg
     1681 aldetmmlrg mryhpidven svlrchkkis ecavftwtnl lvvvveldgn esealdlvpl
     1741 vtsvvleehq lvvgvvvvvd pgvvpinsrg ekqrmhlrdg fladqldpiy vaynm
//
LOCUS       XP_077500284            1766 aa            linear   INV 25-NOV-2025
DEFINITION  disco-interacting protein 2 isoform X4 [Amblyomma americanum].
ACCESSION   XP_077500284
VERSION     XP_077500284.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644158.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1766
                     /product="disco-interacting protein 2 isoform X4"
                     /calculated_mol_wt=192416
     Region          <251..308
                     /region_name="DMAP_binding"
                     /note="DMAP1-binding Domain; pfam06464"
                     /db_xref="CDD:368923"
     Region          533..1108
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(693,696..701,703..704)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(696,816..817,875..880,973,985,988,999,1088)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(696,737..738,788,790..791,794,816..817,835..840,973,
                     985,988,996..999,1069)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(737,790..791,794,816,996..998,1056,1069)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Region          1186..1762
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1334,1337..1342,1344..1345)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1337,1463..1464,1518..1523,1614,1649,1652,1663,1742)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1337,1378..1379,1429,1431..1432,1435,1463..1464,
                     1481..1486,1614,1649,1652,1660..1663,1723)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1378,1431..1432,1435,1463,1660..1662,1710,1723)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     CDS             1..1766
                     /gene="DIP2"
                     /coded_by="XM_077644158.1:16..5316"
                     /db_xref="GeneID:144111035"
ORIGIN      
        1 msgyrialsr evadvvlvlp pfvfrkqgvl hpsgpadykv wpvpapragg dvfplsasvg
       61 dggisrsngr ywtggpvlsl gaasffvrth rwrraqalpv aaaarvvnrr ssgapppgrp
      121 plafplgaew raaggrrrwf saraaaarfa aeriddgqig vrllsppwap rllssgrahv
      181 pswatwsqra amqrprkpsl feapiwkslp yslwaalldd vdeessdded dsesslfssr
      241 vssegclsdt esditqkgye kkrarllapy lpshptevrq eavqqalaam qnkpkpslpm
      301 pskrtsvmtt spsrerhpsd sssdddsvln egstpeqerl mrplptppqs dtsgasspre
      361 tpprialrcs ngrgsdrptv dgvshladvm qnltpseytd lnpqpdvtnt ginanhtpte
      421 hanrynqahm ssvrvtqhsa tgleygtgkw kvsakiqqll ntlkrpkrrp lpefyvddev
      481 dmdsnpsdpn apqpegatmt ptvgenlevp sglpqsleaa ikrygsgtfk fpaatvldts
      541 gkigaplpyg kllsrswkia fnllnklgqk gegqalkpgd rvalvypnnd pvafmcsfyg
      601 clmagivpvp ievpltrrda gsqqigfllg scgvsyalts evcykglpkt atgeihtfkg
      661 wpklhwfvte hlskppkdws pptrltdeta ayieytvdrd gamkgvavsr salvnhsral
      721 taacnytege vmvcvldykr emglwhgvla svfngmhvif ipyslmkvnp aswmlmitkf
      781 kanvavcksr dlhwgllatk dhkdvnlssl rlllvadgsn pwslsscdqf isvfhsrglh
      841 pdavcpcaas pealtvavrr pgrvgagasg rgvlsmaals ygvirvdmen sltsltlqdc
      901 ghilpgavaa vvkindaphl cktdevgeic vsastcgysy wglqglsnni fkmqplhpdg
      961 rpvtdqafvr sgllgfvgpg glvfvcgtrd glmqvsmrkh ntddliatvl avepmkfiyr
     1021 griavfsikv lrdericvva eqrpdcteee sfqwmsrvlq avdsihqvgi yclalvppny
     1081 lpktplggih lpetkrrfle gslhpanvlm cphtcvtnlp kprevhpevg pasvmvgnmv
     1141 qgvrlamaqg rdigiideds dtarkyqfis eilrwrattt adhavftlln akgntasslt
     1201 csqlhkraer vgclllekgk lntgdhvali yppgidlica fygclyvgvv pvtirpphpq
     1261 nlqttlptvr mivdvsksvi vlstapvtkl lrskeasnvv dvkswrmile tddlpkrkls
     1321 siyraptpem iayldfsvst tgmlagikms haaatslcrs mklacelyps rhialcldpy
     1381 cglgfvlwcl nsvysghhsi lippsevevn paiwlttvsq ykvrdtfcsy gvmelctkgl
     1441 gssiaqlkqr nvnlscvrtc vvvaeerpri alttsfsklf sglglsprav stsfgcrvnv
     1501 aiclqgassp dpsqvyvdmr alrndrvtlv ergaphslcl lesgkllpgl klvianpdtr
     1561 gqcgdshlge iwvqsphnas gyftvygdes qhndhfnakl itgdtgsvya rtgylgflrr
     1621 teavssdnet dseaasnvdg pelhdavfvv galdetmmlr gmryhpidve nsvlrchkki
     1681 secavftwtn llvvvveldg nesealdlvp lvtsvvleeh qlvvgvvvvv dpgvvpinsr
     1741 gekqrmhlrd gfladqldpi yvaynm
//
LOCUS       XP_077500293            1629 aa            linear   INV 25-NOV-2025
DEFINITION  disco-interacting protein 2 isoform X5 [Amblyomma americanum].
ACCESSION   XP_077500293
VERSION     XP_077500293.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644167.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1629
                     /product="disco-interacting protein 2 isoform X5"
                     /calculated_mol_wt=177789
     Region          <86..171
                     /region_name="DMAP_binding"
                     /note="DMAP1-binding Domain; pfam06464"
                     /db_xref="CDD:368923"
     Region          396..971
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(556,559..564,566..567)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(559,679..680,738..743,836,848,851,862,951)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(559,600..601,651,653..654,657,679..680,698..703,836,
                     848,851,859..862,932)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(600,653..654,657,679,859..861,919,932)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Region          1049..1625
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1197,1200..1205,1207..1208)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1200,1326..1327,1381..1386,1477,1512,1515,1526,1605)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1200,1241..1242,1292,1294..1295,1298,1326..1327,
                     1344..1349,1477,1512,1515,1523..1526,1586)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1241,1294..1295,1298,1326,1523..1525,1573,1586)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     CDS             1..1629
                     /gene="DIP2"
                     /coded_by="XM_077644167.1:16..4905"
                     /db_xref="GeneID:144111035"
ORIGIN      
        1 mwtarmlaaa eahvfpgfvg rregssgcap fpvprsfcev lprqrrrdvr ygfsrrqarg
       61 tsgapagfac vaargrqahg cdvlrditqk gyekkrarll apylpshpta lptgsaspst
      121 rakrraqrrl trnesryhse vrqeavqqal aamqnkpkps lpmpskrtsv mttspsrerh
      181 psdsssddds vlnegstpeq erlmrplptp pqsdtsgass pretpprial rcsngrgsdr
      241 ptvdgvshla dvmqnltpse ytdlnpqpdv tntginanht ptehanrynq ahmssvrvtq
      301 hsatgleygt gkwkvsakiq qllntlkrpk rrplpefyvd devdmdsnps dpnapqpega
      361 tmtptvgenl evpsglpqsl eaaikrygsg tfkfpaatvl dtsgkigapl pygkllsrsw
      421 kiafnllnkl gqkgegqalk pgdrvalvyp nndpvafmcs fygclmagiv pvpievpltr
      481 rdagsqqigf llgscgvsya ltsevcykgl pktatgeiht fkgwpklhwf vtehlskppk
      541 dwspptrltd etaayieytv drdgamkgva vsrsalvnhs raltaacnyt egevmvcvld
      601 ykremglwhg vlasvfngmh vifipyslmk vnpaswmlmi tkfkanvavc ksrdlhwgll
      661 atkdhkdvnl sslrlllvad gsnpwslssc dqfisvfhsr glhpdavcpc aaspealtva
      721 vrrpgrvgag asgrgvlsma alsygvirvd mensltsltl qdcghilpga vaavvkinda
      781 phlcktdevg eicvsastcg ysywglqgls nnifkmqplh pdgrpvtdqa fvrsgllgfv
      841 gpgglvfvcg trdglmqvsm rkhntddlia tvlavepmkf iyrgriavfs ikvlrderic
      901 vvaeqrpdct eeesfqwmsr vlqavdsihq vgiyclalvp pnylpktplg gihlpetkrr
      961 flegslhpan vlmcphtcvt nlpkprevhp evgpasvmvg nmvqgvrlam aqgrdigiid
     1021 edsdtarkyq fiseilrwra tttadhavft llnakgntas sltcsqlhkr aervgcllle
     1081 kgklntgdhv aliyppgidl icafygclyv gvvpvtirpp hpqnlqttlp tvrmivdvsk
     1141 svivlstapv tkllrskeas nvvdvkswrm iletddlpkr klssiyrapt pemiayldfs
     1201 vsttgmlagi kmshaaatsl crsmklacel ypsrhialcl dpycglgfvl wclnsvysgh
     1261 hsilippsev evnpaiwltt vsqykvrdtf csygvmelct kglgssiaql kqrnvnlscv
     1321 rtcvvvaeer prialttsfs klfsglglsp ravstsfgcr vnvaiclqga sspdpsqvyv
     1381 dmralrndrv tlvergaphs lcllesgkll pglklvianp dtrgqcgdsh lgeiwvqsph
     1441 nasgyftvyg desqhndhfn aklitgdtgs vyartgylgf lrrteavssd netdseaasn
     1501 vdgpelhdav fvvgaldetm mlrgmryhpi dvensvlrch kkisecavft wtnllvvvve
     1561 ldgneseald lvplvtsvvl eehqlvvgvv vvvdpgvvpi nsrgekqrmh lrdgfladql
     1621 dpiyvaynm
//
LOCUS       XP_077500301            1599 aa            linear   INV 25-NOV-2025
DEFINITION  disco-interacting protein 2 isoform X6 [Amblyomma americanum].
ACCESSION   XP_077500301
VERSION     XP_077500301.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644175.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1599
                     /product="disco-interacting protein 2 isoform X6"
                     /calculated_mol_wt=174395
     Region          <86..141
                     /region_name="DMAP_binding"
                     /note="DMAP1-binding Domain; pfam06464"
                     /db_xref="CDD:368923"
     Region          366..941
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(526,529..534,536..537)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(529,649..650,708..713,806,818,821,832,921)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(529,570..571,621,623..624,627,649..650,668..673,806,
                     818,821,829..832,902)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(570,623..624,627,649,829..831,889,902)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Region          1019..1595
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1167,1170..1175,1177..1178)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1170,1296..1297,1351..1356,1447,1482,1485,1496,1575)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1170,1211..1212,1262,1264..1265,1268,1296..1297,
                     1314..1319,1447,1482,1485,1493..1496,1556)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1211,1264..1265,1268,1296,1493..1495,1543,1556)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     CDS             1..1599
                     /gene="DIP2"
                     /coded_by="XM_077644175.1:16..4815"
                     /db_xref="GeneID:144111035"
ORIGIN      
        1 mwtarmlaaa eahvfpgfvg rregssgcap fpvprsfcev lprqrrrdvr ygfsrrqarg
       61 tsgapagfac vaargrqahg cdvlrditqk gyekkrarll apylpshpte vrqeavqqal
      121 aamqnkpkps lpmpskrtsv mttspsrerh psdsssddds vlnegstpeq erlmrplptp
      181 pqsdtsgass pretpprial rcsngrgsdr ptvdgvshla dvmqnltpse ytdlnpqpdv
      241 tntginanht ptehanrynq ahmssvrvtq hsatgleygt gkwkvsakiq qllntlkrpk
      301 rrplpefyvd devdmdsnps dpnapqpega tmtptvgenl evpsglpqsl eaaikrygsg
      361 tfkfpaatvl dtsgkigapl pygkllsrsw kiafnllnkl gqkgegqalk pgdrvalvyp
      421 nndpvafmcs fygclmagiv pvpievpltr rdagsqqigf llgscgvsya ltsevcykgl
      481 pktatgeiht fkgwpklhwf vtehlskppk dwspptrltd etaayieytv drdgamkgva
      541 vsrsalvnhs raltaacnyt egevmvcvld ykremglwhg vlasvfngmh vifipyslmk
      601 vnpaswmlmi tkfkanvavc ksrdlhwgll atkdhkdvnl sslrlllvad gsnpwslssc
      661 dqfisvfhsr glhpdavcpc aaspealtva vrrpgrvgag asgrgvlsma alsygvirvd
      721 mensltsltl qdcghilpga vaavvkinda phlcktdevg eicvsastcg ysywglqgls
      781 nnifkmqplh pdgrpvtdqa fvrsgllgfv gpgglvfvcg trdglmqvsm rkhntddlia
      841 tvlavepmkf iyrgriavfs ikvlrderic vvaeqrpdct eeesfqwmsr vlqavdsihq
      901 vgiyclalvp pnylpktplg gihlpetkrr flegslhpan vlmcphtcvt nlpkprevhp
      961 evgpasvmvg nmvqgvrlam aqgrdigiid edsdtarkyq fiseilrwra tttadhavft
     1021 llnakgntas sltcsqlhkr aervgcllle kgklntgdhv aliyppgidl icafygclyv
     1081 gvvpvtirpp hpqnlqttlp tvrmivdvsk svivlstapv tkllrskeas nvvdvkswrm
     1141 iletddlpkr klssiyrapt pemiayldfs vsttgmlagi kmshaaatsl crsmklacel
     1201 ypsrhialcl dpycglgfvl wclnsvysgh hsilippsev evnpaiwltt vsqykvrdtf
     1261 csygvmelct kglgssiaql kqrnvnlscv rtcvvvaeer prialttsfs klfsglglsp
     1321 ravstsfgcr vnvaiclqga sspdpsqvyv dmralrndrv tlvergaphs lcllesgkll
     1381 pglklvianp dtrgqcgdsh lgeiwvqsph nasgyftvyg desqhndhfn aklitgdtgs
     1441 vyartgylgf lrrteavssd netdseaasn vdgpelhdav fvvgaldetm mlrgmryhpi
     1501 dvensvlrch kkisecavft wtnllvvvve ldgneseald lvplvtsvvl eehqlvvgvv
     1561 vvvdpgvvpi nsrgekqrmh lrdgfladql dpiyvaynm
//
LOCUS       XP_077500309            1572 aa            linear   INV 25-NOV-2025
DEFINITION  disco-interacting protein 2 isoform X7 [Amblyomma americanum].
ACCESSION   XP_077500309
VERSION     XP_077500309.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644183.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1572
                     /product="disco-interacting protein 2 isoform X7"
                     /calculated_mol_wt=171593
     Region          7..114
                     /region_name="DMAP_binding"
                     /note="DMAP1-binding Domain; pfam06464"
                     /db_xref="CDD:368923"
     Region          339..914
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(499,502..507,509..510)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(502,622..623,681..686,779,791,794,805,894)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(502,543..544,594,596..597,600,622..623,641..646,779,
                     791,794,802..805,875)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(543,596..597,600,622,802..804,862,875)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Region          992..1568
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1140,1143..1148,1150..1151)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1143,1269..1270,1324..1329,1420,1455,1458,1469,1548)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1143,1184..1185,1235,1237..1238,1241,1269..1270,
                     1287..1292,1420,1455,1458,1466..1469,1529)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1184,1237..1238,1241,1269,1466..1468,1516,1529)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     CDS             1..1572
                     /gene="DIP2"
                     /coded_by="XM_077644183.1:320..5038"
                     /db_xref="GeneID:144111035"
ORIGIN      
        1 maqdieiasl ppdvreklae lelelsegdi tqkgyekkra rllapylpsh ptalptgsas
       61 pstrakrraq rrltrnesry hsevrqeavq qalaamqnkp kpslpmpskr tsvmttspsr
      121 erhpsdsssd ddsvlnegst peqerlmrpl ptppqsdtsg asspretppr ialrcsngrg
      181 sdrptvdgvs hladvmqnlt pseytdlnpq pdvtntgina nhtptehanr ynqahmssvr
      241 vtqhsatgle ygtgkwkvsa kiqqllntlk rpkrrplpef yvddevdmds npsdpnapqp
      301 egatmtptvg enlevpsglp qsleaaikry gsgtfkfpaa tvldtsgkig aplpygklls
      361 rswkiafnll nklgqkgegq alkpgdrval vypnndpvaf mcsfygclma givpvpievp
      421 ltrrdagsqq igfllgscgv syaltsevcy kglpktatge ihtfkgwpkl hwfvtehlsk
      481 ppkdwspptr ltdetaayie ytvdrdgamk gvavsrsalv nhsraltaac nytegevmvc
      541 vldykremgl whgvlasvfn gmhvifipys lmkvnpaswm lmitkfkanv avcksrdlhw
      601 gllatkdhkd vnlsslrlll vadgsnpwsl sscdqfisvf hsrglhpdav cpcaaspeal
      661 tvavrrpgrv gagasgrgvl smaalsygvi rvdmenslts ltlqdcghil pgavaavvki
      721 ndaphlcktd evgeicvsas tcgysywglq glsnnifkmq plhpdgrpvt dqafvrsgll
      781 gfvgpgglvf vcgtrdglmq vsmrkhntdd liatvlavep mkfiyrgria vfsikvlrde
      841 ricvvaeqrp dcteeesfqw msrvlqavds ihqvgiycla lvppnylpkt plggihlpet
      901 krrflegslh panvlmcpht cvtnlpkpre vhpevgpasv mvgnmvqgvr lamaqgrdig
      961 iidedsdtar kyqfiseilr wratttadha vftllnakgn tassltcsql hkraervgcl
     1021 llekgklntg dhvaliyppg idlicafygc lyvgvvpvti rpphpqnlqt tlptvrmivd
     1081 vsksvivlst apvtkllrsk easnvvdvks wrmiletddl pkrklssiyr aptpemiayl
     1141 dfsvsttgml agikmshaaa tslcrsmkla celypsrhia lcldpycglg fvlwclnsvy
     1201 sghhsilipp sevevnpaiw lttvsqykvr dtfcsygvme lctkglgssi aqlkqrnvnl
     1261 scvrtcvvva eerprialtt sfsklfsglg lspravstsf gcrvnvaicl qgasspdpsq
     1321 vyvdmralrn drvtlverga phslcllesg kllpglklvi anpdtrgqcg dshlgeiwvq
     1381 sphnasgyft vygdesqhnd hfnaklitgd tgsvyartgy lgflrrteav ssdnetdsea
     1441 asnvdgpelh davfvvgald etmmlrgmry hpidvensvl rchkkiseca vftwtnllvv
     1501 vveldgnese aldlvplvts vvleehqlvv gvvvvvdpgv vpinsrgekq rmhlrdgfla
     1561 dqldpiyvay nm
//
LOCUS       XP_077500317            1555 aa            linear   INV 25-NOV-2025
DEFINITION  disco-interacting protein 2 isoform X8 [Amblyomma americanum].
ACCESSION   XP_077500317
VERSION     XP_077500317.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644191.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1555
                     /product="disco-interacting protein 2 isoform X8"
                     /calculated_mol_wt=169500
     Region          7..114
                     /region_name="DMAP_binding"
                     /note="DMAP1-binding Domain; pfam06464"
                     /db_xref="CDD:368923"
     Region          339..914
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(499,502..507,509..510)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(502,622..623,681..686,779,791,794,805,894)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(502,543..544,594,596..597,600,622..623,641..646,779,
                     791,794,802..805,875)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(543,596..597,600,622,802..804,862,875)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Region          992..1528
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1140,1143..1148,1150..1151)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1143,1184..1185,1235,1237..1238,1241,1269..1270,
                     1287..1292,1420,1455,1458,1466..1469)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1143,1269..1270,1324..1329,1420,1455,1458,1469)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1184,1237..1238,1241,1269,1466..1468,1516)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     CDS             1..1555
                     /gene="DIP2"
                     /coded_by="XM_077644191.1:320..4987"
                     /db_xref="GeneID:144111035"
ORIGIN      
        1 maqdieiasl ppdvreklae lelelsegdi tqkgyekkra rllapylpsh ptalptgsas
       61 pstrakrraq rrltrnesry hsevrqeavq qalaamqnkp kpslpmpskr tsvmttspsr
      121 erhpsdsssd ddsvlnegst peqerlmrpl ptppqsdtsg asspretppr ialrcsngrg
      181 sdrptvdgvs hladvmqnlt pseytdlnpq pdvtntgina nhtptehanr ynqahmssvr
      241 vtqhsatgle ygtgkwkvsa kiqqllntlk rpkrrplpef yvddevdmds npsdpnapqp
      301 egatmtptvg enlevpsglp qsleaaikry gsgtfkfpaa tvldtsgkig aplpygklls
      361 rswkiafnll nklgqkgegq alkpgdrval vypnndpvaf mcsfygclma givpvpievp
      421 ltrrdagsqq igfllgscgv syaltsevcy kglpktatge ihtfkgwpkl hwfvtehlsk
      481 ppkdwspptr ltdetaayie ytvdrdgamk gvavsrsalv nhsraltaac nytegevmvc
      541 vldykremgl whgvlasvfn gmhvifipys lmkvnpaswm lmitkfkanv avcksrdlhw
      601 gllatkdhkd vnlsslrlll vadgsnpwsl sscdqfisvf hsrglhpdav cpcaaspeal
      661 tvavrrpgrv gagasgrgvl smaalsygvi rvdmenslts ltlqdcghil pgavaavvki
      721 ndaphlcktd evgeicvsas tcgysywglq glsnnifkmq plhpdgrpvt dqafvrsgll
      781 gfvgpgglvf vcgtrdglmq vsmrkhntdd liatvlavep mkfiyrgria vfsikvlrde
      841 ricvvaeqrp dcteeesfqw msrvlqavds ihqvgiycla lvppnylpkt plggihlpet
      901 krrflegslh panvlmcpht cvtnlpkpre vhpevgpasv mvgnmvqgvr lamaqgrdig
      961 iidedsdtar kyqfiseilr wratttadha vftllnakgn tassltcsql hkraervgcl
     1021 llekgklntg dhvaliyppg idlicafygc lyvgvvpvti rpphpqnlqt tlptvrmivd
     1081 vsksvivlst apvtkllrsk easnvvdvks wrmiletddl pkrklssiyr aptpemiayl
     1141 dfsvsttgml agikmshaaa tslcrsmkla celypsrhia lcldpycglg fvlwclnsvy
     1201 sghhsilipp sevevnpaiw lttvsqykvr dtfcsygvme lctkglgssi aqlkqrnvnl
     1261 scvrtcvvva eerprialtt sfsklfsglg lspravstsf gcrvnvaicl qgasspdpsq
     1321 vyvdmralrn drvtlverga phslcllesg kllpglklvi anpdtrgqcg dshlgeiwvq
     1381 sphnasgyft vygdesqhnd hfnaklitgd tgsvyartgy lgflrrteav ssdnetdsea
     1441 asnvdgpelh davfvvgald etmmlrgmry hpidvensvl rchkkiseca vftwtnllvv
     1501 vveldgnese aldlvplvts vvleehqlvv gvvvvvdpgv vpinsrgvls awtqk
//
LOCUS       XP_077500326            1554 aa            linear   INV 25-NOV-2025
DEFINITION  disco-interacting protein 2 isoform X9 [Amblyomma americanum].
ACCESSION   XP_077500326
VERSION     XP_077500326.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644200.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1554
                     /product="disco-interacting protein 2 isoform X9"
                     /calculated_mol_wt=169398
     Region          7..114
                     /region_name="DMAP_binding"
                     /note="DMAP1-binding Domain; pfam06464"
                     /db_xref="CDD:368923"
     Region          338..913
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(498,501..506,508..509)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(501,621..622,680..685,778,790,793,804,893)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(501,542..543,593,595..596,599,621..622,640..645,778,
                     790,793,801..804,874)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(542,595..596,599,621,801..803,861,874)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Region          991..1527
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1139,1142..1147,1149..1150)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1142,1183..1184,1234,1236..1237,1240,1268..1269,
                     1286..1291,1419,1454,1457,1465..1468)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1142,1268..1269,1323..1328,1419,1454,1457,1468)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1183,1236..1237,1240,1268,1465..1467,1515)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     CDS             1..1554
                     /gene="DIP2"
                     /coded_by="XM_077644200.1:316..4980"
                     /db_xref="GeneID:144111035"
ORIGIN      
        1 maqdieiasl ppdvreklae lelelsegdi tqkgyekkra rllapylpsh ptalptgsas
       61 pstrakrraq rrltrnesry hsevrqeavq qalaamqnkp kpslpmpskr tsvmttspsr
      121 erhpsdsssd ddsvlnegst peqerlmrpl ptppqsdtsg asspretppr ialrcsngrg
      181 sdrptvdgvs hladvmqnlt pseynlnpqp dvtntginan htptehanry nqahmssvrv
      241 tqhsatgley gtgkwkvsak iqqllntlkr pkrrplpefy vddevdmdsn psdpnapqpe
      301 gatmtptvge nlevpsglpq sleaaikryg sgtfkfpaat vldtsgkiga plpygkllsr
      361 swkiafnlln klgqkgegqa lkpgdrvalv ypnndpvafm csfygclmag ivpvpievpl
      421 trrdagsqqi gfllgscgvs yaltsevcyk glpktatgei htfkgwpklh wfvtehlskp
      481 pkdwspptrl tdetaayiey tvdrdgamkg vavsrsalvn hsraltaacn ytegevmvcv
      541 ldykremglw hgvlasvfng mhvifipysl mkvnpaswml mitkfkanva vcksrdlhwg
      601 llatkdhkdv nlsslrlllv adgsnpwsls scdqfisvfh srglhpdavc pcaaspealt
      661 vavrrpgrvg agasgrgvls maalsygvir vdmensltsl tlqdcghilp gavaavvkin
      721 daphlcktde vgeicvsast cgysywglqg lsnnifkmqp lhpdgrpvtd qafvrsgllg
      781 fvgpgglvfv cgtrdglmqv smrkhntddl iatvlavepm kfiyrgriav fsikvlrder
      841 icvvaeqrpd cteeesfqwm srvlqavdsi hqvgiyclal vppnylpktp lggihlpetk
      901 rrflegslhp anvlmcphtc vtnlpkprev hpevgpasvm vgnmvqgvrl amaqgrdigi
      961 idedsdtark yqfiseilrw ratttadhav ftllnakgnt assltcsqlh kraervgcll
     1021 lekgklntgd hvaliyppgi dlicafygcl yvgvvpvtir pphpqnlqtt lptvrmivdv
     1081 sksvivlsta pvtkllrske asnvvdvksw rmiletddlp krklssiyra ptpemiayld
     1141 fsvsttgmla gikmshaaat slcrsmklac elypsrhial cldpycglgf vlwclnsvys
     1201 ghhsilipps evevnpaiwl ttvsqykvrd tfcsygvmel ctkglgssia qlkqrnvnls
     1261 cvrtcvvvae erprialtts fsklfsglgl spravstsfg crvnvaiclq gasspdpsqv
     1321 yvdmralrnd rvtlvergap hslcllesgk llpglklvia npdtrgqcgd shlgeiwvqs
     1381 phnasgyftv ygdesqhndh fnaklitgdt gsvyartgyl gflrrteavs sdnetdseaa
     1441 snvdgpelhd avfvvgalde tmmlrgmryh pidvensvlr chkkisecav ftwtnllvvv
     1501 veldgnesea ldlvplvtsv vleehqlvvg vvvvvdpgvv pinsrgvlsa wtqk
//
LOCUS       XP_077500334            1542 aa            linear   INV 25-NOV-2025
DEFINITION  disco-interacting protein 2 isoform X10 [Amblyomma americanum].
ACCESSION   XP_077500334
VERSION     XP_077500334.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644208.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1542
                     /product="disco-interacting protein 2 isoform X10"
                     /calculated_mol_wt=168199
     Region          7..84
                     /region_name="DMAP_binding"
                     /note="DMAP1-binding Domain; pfam06464"
                     /db_xref="CDD:368923"
     Region          309..884
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(469,472..477,479..480)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(472,592..593,651..656,749,761,764,775,864)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(472,513..514,564,566..567,570,592..593,611..616,749,
                     761,764,772..775,845)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(513,566..567,570,592,772..774,832,845)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Region          962..1538
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1110,1113..1118,1120..1121)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1113,1239..1240,1294..1299,1390,1425,1428,1439,1518)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1113,1154..1155,1205,1207..1208,1211,1239..1240,
                     1257..1262,1390,1425,1428,1436..1439,1499)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1154,1207..1208,1211,1239,1436..1438,1486,1499)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     CDS             1..1542
                     /gene="DIP2"
                     /coded_by="XM_077644208.1:316..4944"
                     /db_xref="GeneID:144111035"
ORIGIN      
        1 maqdieiasl ppdvreklae lelelsegdi tqkgyekkra rllapylpsh ptevrqeavq
       61 qalaamqnkp kpslpmpskr tsvmttspsr erhpsdsssd ddsvlnegst peqerlmrpl
      121 ptppqsdtsg asspretppr ialrcsngrg sdrptvdgvs hladvmqnlt pseytdlnpq
      181 pdvtntgina nhtptehanr ynqahmssvr vtqhsatgle ygtgkwkvsa kiqqllntlk
      241 rpkrrplpef yvddevdmds npsdpnapqp egatmtptvg enlevpsglp qsleaaikry
      301 gsgtfkfpaa tvldtsgkig aplpygklls rswkiafnll nklgqkgegq alkpgdrval
      361 vypnndpvaf mcsfygclma givpvpievp ltrrdagsqq igfllgscgv syaltsevcy
      421 kglpktatge ihtfkgwpkl hwfvtehlsk ppkdwspptr ltdetaayie ytvdrdgamk
      481 gvavsrsalv nhsraltaac nytegevmvc vldykremgl whgvlasvfn gmhvifipys
      541 lmkvnpaswm lmitkfkanv avcksrdlhw gllatkdhkd vnlsslrlll vadgsnpwsl
      601 sscdqfisvf hsrglhpdav cpcaaspeal tvavrrpgrv gagasgrgvl smaalsygvi
      661 rvdmenslts ltlqdcghil pgavaavvki ndaphlcktd evgeicvsas tcgysywglq
      721 glsnnifkmq plhpdgrpvt dqafvrsgll gfvgpgglvf vcgtrdglmq vsmrkhntdd
      781 liatvlavep mkfiyrgria vfsikvlrde ricvvaeqrp dcteeesfqw msrvlqavds
      841 ihqvgiycla lvppnylpkt plggihlpet krrflegslh panvlmcpht cvtnlpkpre
      901 vhpevgpasv mvgnmvqgvr lamaqgrdig iidedsdtar kyqfiseilr wratttadha
      961 vftllnakgn tassltcsql hkraervgcl llekgklntg dhvaliyppg idlicafygc
     1021 lyvgvvpvti rpphpqnlqt tlptvrmivd vsksvivlst apvtkllrsk easnvvdvks
     1081 wrmiletddl pkrklssiyr aptpemiayl dfsvsttgml agikmshaaa tslcrsmkla
     1141 celypsrhia lcldpycglg fvlwclnsvy sghhsilipp sevevnpaiw lttvsqykvr
     1201 dtfcsygvme lctkglgssi aqlkqrnvnl scvrtcvvva eerprialtt sfsklfsglg
     1261 lspravstsf gcrvnvaicl qgasspdpsq vyvdmralrn drvtlverga phslcllesg
     1321 kllpglklvi anpdtrgqcg dshlgeiwvq sphnasgyft vygdesqhnd hfnaklitgd
     1381 tgsvyartgy lgflrrteav ssdnetdsea asnvdgpelh davfvvgald etmmlrgmry
     1441 hpidvensvl rchkkiseca vftwtnllvv vveldgnese aldlvplvts vvleehqlvv
     1501 gvvvvvdpgv vpinsrgekq rmhlrdgfla dqldpiyvay nm
//
LOCUS       XP_077500341            1541 aa            linear   INV 25-NOV-2025
DEFINITION  disco-interacting protein 2 isoform X11 [Amblyomma americanum].
ACCESSION   XP_077500341
VERSION     XP_077500341.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644215.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1541
                     /product="disco-interacting protein 2 isoform X11"
                     /calculated_mol_wt=168097
     Region          7..84
                     /region_name="DMAP_binding"
                     /note="DMAP1-binding Domain; pfam06464"
                     /db_xref="CDD:368923"
     Region          308..883
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(468,471..476,478..479)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(471,591..592,650..655,748,760,763,774,863)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(471,512..513,563,565..566,569,591..592,610..615,748,
                     760,763,771..774,844)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(512,565..566,569,591,771..773,831,844)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Region          961..1537
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1109,1112..1117,1119..1120)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1112,1238..1239,1293..1298,1389,1424,1427,1438,1517)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1112,1153..1154,1204,1206..1207,1210,1238..1239,
                     1256..1261,1389,1424,1427,1435..1438,1498)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1153,1206..1207,1210,1238,1435..1437,1485,1498)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     CDS             1..1541
                     /gene="DIP2"
                     /coded_by="XM_077644215.1:316..4941"
                     /db_xref="GeneID:144111035"
ORIGIN      
        1 maqdieiasl ppdvreklae lelelsegdi tqkgyekkra rllapylpsh ptevrqeavq
       61 qalaamqnkp kpslpmpskr tsvmttspsr erhpsdsssd ddsvlnegst peqerlmrpl
      121 ptppqsdtsg asspretppr ialrcsngrg sdrptvdgvs hladvmqnlt pseynlnpqp
      181 dvtntginan htptehanry nqahmssvrv tqhsatgley gtgkwkvsak iqqllntlkr
      241 pkrrplpefy vddevdmdsn psdpnapqpe gatmtptvge nlevpsglpq sleaaikryg
      301 sgtfkfpaat vldtsgkiga plpygkllsr swkiafnlln klgqkgegqa lkpgdrvalv
      361 ypnndpvafm csfygclmag ivpvpievpl trrdagsqqi gfllgscgvs yaltsevcyk
      421 glpktatgei htfkgwpklh wfvtehlskp pkdwspptrl tdetaayiey tvdrdgamkg
      481 vavsrsalvn hsraltaacn ytegevmvcv ldykremglw hgvlasvfng mhvifipysl
      541 mkvnpaswml mitkfkanva vcksrdlhwg llatkdhkdv nlsslrlllv adgsnpwsls
      601 scdqfisvfh srglhpdavc pcaaspealt vavrrpgrvg agasgrgvls maalsygvir
      661 vdmensltsl tlqdcghilp gavaavvkin daphlcktde vgeicvsast cgysywglqg
      721 lsnnifkmqp lhpdgrpvtd qafvrsgllg fvgpgglvfv cgtrdglmqv smrkhntddl
      781 iatvlavepm kfiyrgriav fsikvlrder icvvaeqrpd cteeesfqwm srvlqavdsi
      841 hqvgiyclal vppnylpktp lggihlpetk rrflegslhp anvlmcphtc vtnlpkprev
      901 hpevgpasvm vgnmvqgvrl amaqgrdigi idedsdtark yqfiseilrw ratttadhav
      961 ftllnakgnt assltcsqlh kraervgcll lekgklntgd hvaliyppgi dlicafygcl
     1021 yvgvvpvtir pphpqnlqtt lptvrmivdv sksvivlsta pvtkllrske asnvvdvksw
     1081 rmiletddlp krklssiyra ptpemiayld fsvsttgmla gikmshaaat slcrsmklac
     1141 elypsrhial cldpycglgf vlwclnsvys ghhsilipps evevnpaiwl ttvsqykvrd
     1201 tfcsygvmel ctkglgssia qlkqrnvnls cvrtcvvvae erprialtts fsklfsglgl
     1261 spravstsfg crvnvaiclq gasspdpsqv yvdmralrnd rvtlvergap hslcllesgk
     1321 llpglklvia npdtrgqcgd shlgeiwvqs phnasgyftv ygdesqhndh fnaklitgdt
     1381 gsvyartgyl gflrrteavs sdnetdseaa snvdgpelhd avfvvgalde tmmlrgmryh
     1441 pidvensvlr chkkisecav ftwtnllvvv veldgnesea ldlvplvtsv vleehqlvvg
     1501 vvvvvdpgvv pinsrgekqr mhlrdgflad qldpiyvayn m
//
LOCUS       XP_077500348            1571 aa            linear   INV 25-NOV-2025
DEFINITION  disco-interacting protein 2 isoform X12 [Amblyomma americanum].
ACCESSION   XP_077500348
VERSION     XP_077500348.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644222.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1571
                     /product="disco-interacting protein 2 isoform X12"
                     /calculated_mol_wt=171491
     Region          7..114
                     /region_name="DMAP_binding"
                     /note="DMAP1-binding Domain; pfam06464"
                     /db_xref="CDD:368923"
     Region          338..913
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(498,501..506,508..509)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(501,621..622,680..685,778,790,793,804,893)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(501,542..543,593,595..596,599,621..622,640..645,778,
                     790,793,801..804,874)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(542,595..596,599,621,801..803,861,874)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Region          991..1567
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1139,1142..1147,1149..1150)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1142,1268..1269,1323..1328,1419,1454,1457,1468,1547)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1142,1183..1184,1234,1236..1237,1240,1268..1269,
                     1286..1291,1419,1454,1457,1465..1468,1528)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1183,1236..1237,1240,1268,1465..1467,1515,1528)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     CDS             1..1571
                     /gene="DIP2"
                     /coded_by="XM_077644222.1:316..5031"
                     /db_xref="GeneID:144111035"
ORIGIN      
        1 maqdieiasl ppdvreklae lelelsegdi tqkgyekkra rllapylpsh ptalptgsas
       61 pstrakrraq rrltrnesry hsevrqeavq qalaamqnkp kpslpmpskr tsvmttspsr
      121 erhpsdsssd ddsvlnegst peqerlmrpl ptppqsdtsg asspretppr ialrcsngrg
      181 sdrptvdgvs hladvmqnlt pseynlnpqp dvtntginan htptehanry nqahmssvrv
      241 tqhsatgley gtgkwkvsak iqqllntlkr pkrrplpefy vddevdmdsn psdpnapqpe
      301 gatmtptvge nlevpsglpq sleaaikryg sgtfkfpaat vldtsgkiga plpygkllsr
      361 swkiafnlln klgqkgegqa lkpgdrvalv ypnndpvafm csfygclmag ivpvpievpl
      421 trrdagsqqi gfllgscgvs yaltsevcyk glpktatgei htfkgwpklh wfvtehlskp
      481 pkdwspptrl tdetaayiey tvdrdgamkg vavsrsalvn hsraltaacn ytegevmvcv
      541 ldykremglw hgvlasvfng mhvifipysl mkvnpaswml mitkfkanva vcksrdlhwg
      601 llatkdhkdv nlsslrlllv adgsnpwsls scdqfisvfh srglhpdavc pcaaspealt
      661 vavrrpgrvg agasgrgvls maalsygvir vdmensltsl tlqdcghilp gavaavvkin
      721 daphlcktde vgeicvsast cgysywglqg lsnnifkmqp lhpdgrpvtd qafvrsgllg
      781 fvgpgglvfv cgtrdglmqv smrkhntddl iatvlavepm kfiyrgriav fsikvlrder
      841 icvvaeqrpd cteeesfqwm srvlqavdsi hqvgiyclal vppnylpktp lggihlpetk
      901 rrflegslhp anvlmcphtc vtnlpkprev hpevgpasvm vgnmvqgvrl amaqgrdigi
      961 idedsdtark yqfiseilrw ratttadhav ftllnakgnt assltcsqlh kraervgcll
     1021 lekgklntgd hvaliyppgi dlicafygcl yvgvvpvtir pphpqnlqtt lptvrmivdv
     1081 sksvivlsta pvtkllrske asnvvdvksw rmiletddlp krklssiyra ptpemiayld
     1141 fsvsttgmla gikmshaaat slcrsmklac elypsrhial cldpycglgf vlwclnsvys
     1201 ghhsilipps evevnpaiwl ttvsqykvrd tfcsygvmel ctkglgssia qlkqrnvnls
     1261 cvrtcvvvae erprialtts fsklfsglgl spravstsfg crvnvaiclq gasspdpsqv
     1321 yvdmralrnd rvtlvergap hslcllesgk llpglklvia npdtrgqcgd shlgeiwvqs
     1381 phnasgyftv ygdesqhndh fnaklitgdt gsvyartgyl gflrrteavs sdnetdseaa
     1441 snvdgpelhd avfvvgalde tmmlrgmryh pidvensvlr chkkisecav ftwtnllvvv
     1501 veldgnesea ldlvplvtsv vleehqlvvg vvvvvdpgvv pinsrgekqr mhlrdgflad
     1561 qldpiyvayn m
//
LOCUS       XP_077500358            1722 aa            linear   INV 25-NOV-2025
DEFINITION  tyrosine-protein phosphatase non-receptor type protein myopic
            [Amblyomma americanum].
ACCESSION   XP_077500358
VERSION     XP_077500358.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644232.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1722
                     /product="tyrosine-protein phosphatase non-receptor type
                     protein myopic"
                     /calculated_mol_wt=186988
     Region          2..367
                     /region_name="BRO1_HD-PTP_like"
                     /note="Protein-interacting, N-terminal, Bro1-like domain
                     of mammalian His-Domain type N23 protein tyrosine
                     phosphatase and related domains; cd09239"
                     /db_xref="CDD:185762"
     Site            order(126,129,142,145..146,190,193..194,203,206..207,346,
                     348,350,352)
                     /site_type="other"
                     /note="putative ESCRT-III binding site"
                     /db_xref="CDD:185762"
     Region          372..706
                     /region_name="V_Alix_like"
                     /note="Protein-interacting V-domain of mammalian Alix and
                     related domains; cl14654"
                     /db_xref="CDD:353824"
     Site            order(453,507,511,514..515,677,680..681,684,687)
                     /site_type="other"
                     /note="putative YPXnL-motif binding site"
                     /db_xref="CDD:185746"
     Region          <933..>1200
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          1316..1518
                     /region_name="PTP_DSP_cys"
                     /note="cys-based protein tyrosine phosphatase and
                     dual-specificity phosphatase superfamily; cl28904"
                     /db_xref="CDD:475123"
     CDS             1..1722
                     /gene="mop"
                     /coded_by="XM_077644232.1:73..5241"
                     /db_xref="GeneID:144111089"
ORIGIN      
        1 mesaprlpml scelknspsn aefgpalkky iaehyledpd synneirele alrmaackvs
       61 rdftgcsvlk ryysqllclq srfpmtdega acvpfmwtdi ysgmvfnimd ikyeqasily
      121 nigalhsklg alenrssseg mkiacthfqc aawalsqvkd lfpqpkgsdm shdlllffvn
      181 iflgqaqeci leksmldrrk ssitakvaaq vveyfraavs lllagssssd sgsiqeivgs
      241 kltklwkkil dfkmayylsv sclhmgnqae eaqkmgerqa wyqlavehln eatsiakgle
      301 eenlsetlsf amdviggkfk aakkenefvy hekvppvesl pevkganlvk gisfsptdpe
      361 isgpdifarl vpieaheass iyseekarll rsvtsrvekk neeleaflss lnldksmlvm
      421 daqavpqevv qccarlsvcp tavsdlvlti qglkelyhsv dkdlaqveql lkeerekeaa
      481 hgtalgkrap snlvtelaae lakyqevhqr tadsnsalqk slaahsgnlk llsspleevd
      541 kalpsvslld vpnneavvke lshlldkvde mkkqrqmlhs hlrdalqkdd vtrqivtrnk
      601 geelqaffaa elkkhdqevs lleqnlaaqd nilkaltqan arfaetrkaa sdilrrream
      661 vasliasfea yedlgtktqk gvefykkmqt nvgklltrlr svvkvqdeer alqleaqark
      721 agklgslpss saggmklkdf lpymtgqglp lpqeasgtsa yysaagytgy qtpqvasqvs
      781 tdkgptetpd kvgdamgmgv rptplgseqt spqisgrtdg yapsayktsf pdsysssvdk
      841 ygkpvlpqmp assslqpgis ntslayqgvy tgpssistal sggnsaaisi erapvaarpe
      901 yrsyteytps gqynlptspa stltytfrnt nlmlqgpesv pppessgyla stnntyaygq
      961 svmssqqgiq qglpyqpypa asvsaasqhv ppvyyglgyg qhpstlsiqa pvsyaqsynp
     1021 qqpgaqastp vqqpasgdnl gasmapvymv ypsnyyasnt dqsgaqqqla fqasgpqsad
     1081 lpagqvyans wpyqpsqmmq ypssavsasg taavveptag avplqpvvaa psaasvvppa
     1141 ppavgiarea qdlatqslqp gsfvmdstlp lvpeaakkdl lsatldstqn aksqaavlqp
     1201 qvlsnenwke rggkadekpt eartsgkdpl sdpvalekfv teverfekyv egltkkslng
     1261 ptlleqkwke yldaqdresr klsisvarcy plknripdvm pydhnrvamt sqrddyinas
     1321 fvkgltpfcl syiltqaplp atfsdfwami weqqtetvvc lqnnlelksh tywpvekgqq
     1381 irhgtitlsl qsikekqfyi erminiansd npkvsrvvih lqlldwphsg spaspaqlln
     1441 fisevhsfhk tqrnhsrpiv vhclggvgrs gvfclvsaam keiaagngll dilstaikla
     1501 qsrklciqek eqfkfchdiv lyhaqdllik rgiltstpsf gtkahtgtps hvrhpsedfm
     1561 lrsgstgqra stvpdapapt aaapttasdt seqvtstepg steppvaaet elstsetpke
     1621 isnnhaaeqv tsgitdelps tasamdvvpp apegkdsgat avadlldpat ftldpghgtp
     1681 akqkitresf mnaqgtlsqs kpdpkdplsq ldplwslkkg ke
//
LOCUS       XP_077500367             364 aa            linear   INV 25-NOV-2025
DEFINITION  protein CNPPD1 [Amblyomma americanum].
ACCESSION   XP_077500367
VERSION     XP_077500367.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644241.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..364
                     /product="protein CNPPD1"
                     /calculated_mol_wt=40116
     Region          76..169
                     /region_name="CYCLIN_ScPCL1-like"
                     /note="cyclin box found in Saccharomyces cerevisiae
                     G1/S-specific cyclin PCL1, PCL2 and similar proteins;
                     cd20557"
                     /db_xref="CDD:410260"
     Site            order(91,129,141..143,157,160..161)
                     /site_type="other"
                     /note="putative CDK interface [polypeptide binding]"
                     /db_xref="CDD:410260"
     CDS             1..364
                     /gene="LOC144111100"
                     /coded_by="XM_077644241.1:133..1227"
                     /db_xref="GeneID:144111100"
ORIGIN      
        1 mnlfhalsfd ndqedlllga diqifgdhve lsdrirktfy ygklpttdrp slaltgisve
       61 mfskvlpndg lkvldmhyaa svsrracitp csmmlamvyl dqlrhkspqy itsvsscdlf
      121 lvsmmvaskf lyddgeedev fnnewaasan velkdlnvle refldaldwn lyvkpkafah
      181 vldsletria ylestkrgwt tytdvcvlgk gavlercwll vydgvikvia vsaiayvaaa
      241 ltlfgsaalv hkvahtshql llctslgsqr isfvpaqsnt savlpcvvpp flssstdqdg
      301 qasiectlds pprvsskpsr qhsktaihlp arssastrpr hqrvckgslr yplvancvev
      361 tfsl
//
LOCUS       XP_077500378             787 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111111 [Amblyomma americanum].
ACCESSION   XP_077500378
VERSION     XP_077500378.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644252.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..787
                     /product="uncharacterized protein LOC144111111"
                     /calculated_mol_wt=89441
     Region          26..47
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(26,29,42,47)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          58..77
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          316..409
                     /region_name="MULE"
                     /note="MULE transposase domain; pfam10551"
                     /db_xref="CDD:463145"
     CDS             1..787
                     /gene="LOC144111111"
                     /coded_by="XM_077644252.1:217..2580"
                     /db_xref="GeneID:144111111"
ORIGIN      
        1 masvfpnqse rqpehgdhdl hhdrrcgvcd kvftqrknml qhmrmvhkle tkgallrcdq
       61 cssllstlen lvhhynvahk fpaenlqlsf gndkefhdwk gqeeasqvck fvistgekrl
      121 tnectrtyya cnrsgvarpg rgnktramks qgscrsgrtc lsflivtrde gtvkvsyqrt
      181 hyghdadveh lsmtdqeras iaddlskgvp mrtilkkire svtskltplh ltnrmdlhni
      241 krqynltvpe rchdndamsv siwvqsmrnk gqpvvrlyke qavvdptntf stkdfalalm
      301 tepqeellkv lgtgtvcvds thgttgyqfe lvtvlvldey gaglpmayfi cstinektls
      361 affeslksst galsartfmt ddaseyfkaw stvmgppqhr llctwhvdks wkrrisekik
      421 gkelavdvyh avrtllecpk kdevaelles flankdprlq efiqyfrgny ahrlemways
      481 frtglgintn mylermhrtl khsflegkkn hrvdrlital mdmtyeslmk rvqhivkakr
      541 tskmsriekn hrsavaittp akrnedgtwt vqsqstrglt ymiskekgsa ccplkcsecm
      601 vcvhsyscdc eehrmsltic khihcvviag apegrdqkil kateavssns kaneafhile
      661 svqtaeasts mtksdlvlak mellkeyvae qeipqevhnk asklldpvlk iyegtkkqem
      721 phtsrepank kiehqvrfhs tkkqrlsssg tsfskptelq kenikkqiln sndeatviit
      781 spghnyi
//
LOCUS       XP_077500394             319 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111122 [Amblyomma americanum].
ACCESSION   XP_077500394
VERSION     XP_077500394.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644268.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..319
                     /product="uncharacterized protein LOC144111122"
                     /calculated_mol_wt=35073
     CDS             1..319
                     /gene="LOC144111122"
                     /coded_by="XM_077644268.1:696..1655"
                     /db_xref="GeneID:144111122"
ORIGIN      
        1 mpkrkckfte qmgkeysfik ktkseadvrc dacgadfsva hggrsdivkh issvkhkqsv
       61 saaarsnavt qffrqerage kelqlaaskg vfafhtiahn hsfrsmdcts kllqklfnek
      121 sscartkaea ialnvlapsa eeelktdlqe akfisvfcdp snhkdlkvlp imvryftaaa
      181 gvqtkllein tlpgktsamv sqyimdalea nqvtsklvaf sadnancnfg gaarngknnv
      241 fsrsqdsmdh kivgigcaah iahntvqtaa dclpldieav ivkiysyfyi ytvimesfkk
      301 fcdfasaeyq rllgysktk
//
LOCUS       XP_077500405             117 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111128 [Amblyomma americanum].
ACCESSION   XP_077500405
VERSION     XP_077500405.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644279.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..117
                     /product="uncharacterized protein LOC144111128"
                     /calculated_mol_wt=13694
     CDS             1..117
                     /gene="LOC144111128"
                     /coded_by="XM_077644279.1:360..713"
                     /db_xref="GeneID:144111128"
ORIGIN      
        1 menlttstvh sqdrlrhprp mrrrrresth cssdntcycr tceadaaetd rhfnwvtwcp
       61 mylllaaphf lgclpinddg rvkeslehgd dpsethrrlr tssncwhhws rydtqva
//
LOCUS       XP_077500435             319 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111150 [Amblyomma americanum].
ACCESSION   XP_077500435
VERSION     XP_077500435.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644309.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..319
                     /product="uncharacterized protein LOC144111150"
                     /calculated_mol_wt=35100
     CDS             1..319
                     /gene="LOC144111150"
                     /coded_by="XM_077644309.1:1684..2643"
                     /db_xref="GeneID:144111150"
ORIGIN      
        1 mpkrkckfte qmgkeysfik ktkseadvrc dacgadfsva hggrsdivkh issvkhkqsv
       61 saaarsnavt qffrqerage kelqlaaskg vfafhtiahn hsfrsmdcts kllqklfnkk
      121 sscartkaea ialnvlapsa eeelktdlqe akfisvfcdp snhkdlkvlp imvryftaaa
      181 gvqtkllein tlpgktsamv sqyimdalea nqvtsklvaf sadnancnfg gaarngknnv
      241 fsrsqdsmdh kivgigcaah iahntvqtaa dclpldieav ivkiysyfyi ytvimesfkk
      301 fcdfasaeyq rllgysktr
//
LOCUS       XP_077500447             160 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111159 [Amblyomma americanum].
ACCESSION   XP_077500447
VERSION     XP_077500447.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644321.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..160
                     /product="uncharacterized protein LOC144111159"
                     /calculated_mol_wt=18336
     CDS             1..160
                     /gene="LOC144111159"
                     /coded_by="XM_077644321.1:876..1358"
                     /db_xref="GeneID:144111159"
ORIGIN      
        1 mhlirilygn trdarlcaai vaelnaglpp gegpcnnkqv klkmenltts tvhsqdrlrh
       61 prpmrrrrre sthcssdntc ycrtceadaa etdrhfnwvt wcpmylllaa phflgclpin
      121 ddgrvkesle hgddpsethr rlrtssncwh hwsrydtqva
//
LOCUS       XP_077500468             137 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111173 [Amblyomma americanum].
ACCESSION   XP_077500468
VERSION     XP_077500468.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644342.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..137
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..137
                     /product="uncharacterized protein LOC144111173"
                     /calculated_mol_wt=15054
     Region          15..97
                     /region_name="DUF4806"
                     /note="Domain of unknown function (DUF4806); pfam16064"
                     /db_xref="CDD:465001"
     CDS             1..137
                     /gene="LOC144111173"
                     /coded_by="XM_077644342.1:77..490"
                     /db_xref="GeneID:144111173"
ORIGIN      
        1 magrlsrdvv vsreqnplit tpfkdyealk efdvtlhgsk redfiqelsg kgganvtsst
       61 rrllrhlled slacqfswlg kkgkhafndl rlcsclisai krghdandyv vgaaihewlr
      121 haparhcsat gknnavg
//
LOCUS       XP_077500480             505 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111177 [Amblyomma americanum].
ACCESSION   XP_077500480
VERSION     XP_077500480.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644354.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..505
                     /product="uncharacterized protein LOC144111177"
                     /calculated_mol_wt=53957
     Region          29..>103
                     /region_name="ASH"
                     /note="Abnormal spindle-like microcephaly-assoc'd,
                     ASPM-SPD-2-Hydin; pfam15780"
                     /db_xref="CDD:464865"
     CDS             1..505
                     /gene="LOC144111177"
                     /coded_by="XM_077644354.1:209..1726"
                     /db_xref="GeneID:144111177"
ORIGIN      
        1 magklltfsp pskvpskvve gravdaevpi lllqpfsksp qvtfeqipvg qeavrqlkvs
       61 npsntdhmvt lsdtlrskgf radpevfkll pgqsqlvsff wkpngnetss rlavpistdk
      121 gykgrfvllg tvreepkplk pprknlvkss iltssqkpnl ssrattsvqt pkkvnstpar
      181 ackkavppir atipkefkss anknagrenl tskvsasaqv skkaatamle pckktvppvr
      241 ftvpkcfasr pnknatsnrp assslpasit cdpliggdaq sllgggtisv vpasnksggl
      301 yplktatcte eirremfacg apaleatanv tvedsedsfk tanatfensl emsknafnds
      361 lepgkladst avdaaatvnn seehsdfgng nndtfvldls aemlyqqiie kqscassqke
      421 epittsaeqa kpllctpqea diegslwppq lqaisneqrs sspkcpakas kedslpdien
      481 lltelelvag rdprcsvdds lleml
//
LOCUS       XP_077500494             319 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111185 [Amblyomma americanum].
ACCESSION   XP_077500494
VERSION     XP_077500494.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644368.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..319
                     /product="uncharacterized protein LOC144111185"
                     /calculated_mol_wt=35073
     CDS             1..319
                     /gene="LOC144111185"
                     /coded_by="XM_077644368.1:1..960"
                     /db_xref="GeneID:144111185"
ORIGIN      
        1 mpkrkckfte qmgkeysfik ktkseadvrc dacgadfsva hggrsdivkh issvkhkqsv
       61 saaarsnavt qffrqerage kelqlaaskg vfafhtiahn hsfrsmdcts kllqklfnek
      121 sscartkaea ialnvlapsa eeelktdlqe akfisvfcdp snhkdlkvlp imvryftaaa
      181 gvqtkllein tlpgktsamv sqyimdalea nqvtsklvaf sadnancnfg gaarngknnv
      241 fsrsqdsmdh kivgigcaah iahntvqtaa dclpldieav ivkiysyfyi ytvimesfkk
      301 fcdfasaeyq rllgysktk
//
LOCUS       XP_077500507             117 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111190 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077500507
VERSION     XP_077500507.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644381.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..117
                     /product="uncharacterized protein LOC144111190 isoform X1"
                     /calculated_mol_wt=13694
     CDS             1..117
                     /gene="LOC144111190"
                     /coded_by="XM_077644381.1:392..745"
                     /db_xref="GeneID:144111190"
ORIGIN      
        1 menlttstvh sqdrlrhprp mrrrrresth cssdntcycr tceadaaetd rhfnwvtwcp
       61 mylllaaphf lgclpinddg rvkeslehgd dpsethrrlr tssncwhhws rydtqva
//
LOCUS       XP_077500516             105 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111190 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077500516
VERSION     XP_077500516.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644390.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..105
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..105
                     /product="uncharacterized protein LOC144111190 isoform X2"
                     /calculated_mol_wt=11995
     CDS             1..105
                     /gene="LOC144111190"
                     /coded_by="XM_077644390.1:1005..1322"
                     /db_xref="GeneID:144111190"
ORIGIN      
        1 menlttstvh sqdrlrhprp mrrrrresth cssdntcycr tceadaaetd rhfnwvtwcp
       61 mylllaaphf lgclpinddg rvkeslevgi icqkahfvdg fdssl
//
LOCUS       XP_077500529             505 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111204 [Amblyomma americanum].
ACCESSION   XP_077500529
VERSION     XP_077500529.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644403.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..505
                     /product="uncharacterized protein LOC144111204"
                     /calculated_mol_wt=53909
     Region          29..>103
                     /region_name="ASH"
                     /note="Abnormal spindle-like microcephaly-assoc'd,
                     ASPM-SPD-2-Hydin; pfam15780"
                     /db_xref="CDD:464865"
     CDS             1..505
                     /gene="LOC144111204"
                     /coded_by="XM_077644403.1:209..1726"
                     /db_xref="GeneID:144111204"
ORIGIN      
        1 magklltfsp pskvpskvve gravdaevpi lllqpfsksp qvtfeqipvg qeavrqlkvs
       61 npsntdhmvt lsdtlrskgf radpevfkll pgqsqlvsff wkpngnetss rlavpistdk
      121 gykgrfvllg tvreepkplk pprknlvkss iltssqkpnl ssrattsvqt pkkvnstpar
      181 ackkavppir atipkefkss anknagrenl tskvsasaqv skkaatamle pckktvppvr
      241 vtvpkcfasr pnknatsnrp assslpasit cdpliggdaq sllgggtisv vpasnksggl
      301 yplktatcte eirremfacg apaleatanv tvedsedsfk tanatfensl emsknafnds
      361 lepgkladst avdaaatvnn seehsdfgng nndtfvldls aemlyqqiie kqscassqke
      421 epittsaeqa kpllctpqea diegslwppq lqaisneqrs sspkcpakas kedslpdien
      481 lltelelvag rdprcsvdds lleml
//
LOCUS       XP_077500544             319 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111205 [Amblyomma americanum].
ACCESSION   XP_077500544
VERSION     XP_077500544.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644418.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..319
                     /product="uncharacterized protein LOC144111205"
                     /calculated_mol_wt=35073
     CDS             1..319
                     /gene="LOC144111205"
                     /coded_by="XM_077644418.1:1685..2644"
                     /db_xref="GeneID:144111205"
ORIGIN      
        1 mpkrkckfte qmgkeysfik ktkseadvrc dacgadfsva hggrsdivkh issvkhkqsv
       61 saaarsnavt qffrqerage kelqlaaskg vfafhtiahn hsfrsmdcts kllqklfnek
      121 sscartkaea ialnvlapsa eeelktdlqe akfisvfcdp snhkdlkvlp imvryftaaa
      181 gvqtkllein tlpgktsamv sqyimdalea nqvtsklvaf sadnancnfg gaarngknnv
      241 fsrsqdsmdh kivgigcaah iahntvqtaa dclpldieav ivkiysyfyi ytvimesfkk
      301 fcdfasaeyq rllgysktk
//
LOCUS       XP_077500555             117 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111214 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077500555
VERSION     XP_077500555.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644429.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..117
                     /product="uncharacterized protein LOC144111214 isoform X1"
                     /calculated_mol_wt=13694
     CDS             1..117
                     /gene="LOC144111214"
                     /coded_by="XM_077644429.1:355..708"
                     /db_xref="GeneID:144111214"
ORIGIN      
        1 menlttstvh sqdrlrhprp mrrrrresth cssdntcycr tceadaaetd rhfnwvtwcp
       61 mylllaaphf lgclpinddg rvkeslehgd dpsethrrlr tssncwhhws rydtqva
//
LOCUS       XP_077500563             117 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111214 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077500563
VERSION     XP_077500563.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644437.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..117
                     /product="uncharacterized protein LOC144111214 isoform X1"
                     /calculated_mol_wt=13694
     CDS             1..117
                     /gene="LOC144111214"
                     /coded_by="XM_077644437.1:427..780"
                     /db_xref="GeneID:144111214"
ORIGIN      
        1 menlttstvh sqdrlrhprp mrrrrresth cssdntcycr tceadaaetd rhfnwvtwcp
       61 mylllaaphf lgclpinddg rvkeslehgd dpsethrrlr tssncwhhws rydtqva
//
LOCUS       XP_077500569             105 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111214 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077500569
VERSION     XP_077500569.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644443.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..105
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..105
                     /product="uncharacterized protein LOC144111214 isoform X2"
                     /calculated_mol_wt=11995
     CDS             1..105
                     /gene="LOC144111214"
                     /coded_by="XM_077644443.1:356..673"
                     /db_xref="GeneID:144111214"
ORIGIN      
        1 menlttstvh sqdrlrhprp mrrrrresth cssdntcycr tceadaaetd rhfnwvtwcp
       61 mylllaaphf lgclpinddg rvkeslevgi icqkahfvdg fdssl
//
LOCUS       XP_077500581             137 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111233 [Amblyomma americanum].
ACCESSION   XP_077500581
VERSION     XP_077500581.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644455.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..137
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..137
                     /product="uncharacterized protein LOC144111233"
                     /calculated_mol_wt=15054
     Region          15..97
                     /region_name="DUF4806"
                     /note="Domain of unknown function (DUF4806); pfam16064"
                     /db_xref="CDD:465001"
     CDS             1..137
                     /gene="LOC144111233"
                     /coded_by="XM_077644455.1:77..490"
                     /db_xref="GeneID:144111233"
ORIGIN      
        1 magrlsrdvv vsreqnplit tpfkdyealk efdvtlhgsk redfiqelsg kgganvtsst
       61 rrllrhlled slacqfswlg kkgkhafndl rlcsclisai krghdandyv vgaaihewlr
      121 haparhcsat gknnavg
//
LOCUS       XP_077500594             505 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111243 [Amblyomma americanum].
ACCESSION   XP_077500594
VERSION     XP_077500594.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644468.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..505
                     /product="uncharacterized protein LOC144111243"
                     /calculated_mol_wt=53909
     Region          29..>103
                     /region_name="ASH"
                     /note="Abnormal spindle-like microcephaly-assoc'd,
                     ASPM-SPD-2-Hydin; pfam15780"
                     /db_xref="CDD:464865"
     CDS             1..505
                     /gene="LOC144111243"
                     /coded_by="XM_077644468.1:209..1726"
                     /db_xref="GeneID:144111243"
ORIGIN      
        1 magklltfsp pskvpskvve gravdaevpi lllqpfsksp qvtfeqipvg qeavrqlkvs
       61 npsntdhmvt lsdtlrskgf radpevfkll pgqsqlvsff wkpngnetss rlavpistdk
      121 gykgrfvllg tvreepkplk pprknlvkss iltssqkpnl ssrattsvqt pkkvnstpar
      181 ackkavppir atipkefkss anknagrenl tskvsasaqv skkaatamle pckktvppvr
      241 vtvpkcfasr pnknatsnrp assslpasit cdpliggdaq sllgggtisv vpasnksggl
      301 yplktatcte eirremfacg apaleatanv tvedsedsfk tanatfensl emsknafnds
      361 lepgkladst avdaaatvnn seehsdfgng nndtfvldls aemlyqqiie kqscassqke
      421 epittsaeqa kpllctpqea diegslwppq lqaisneqrs sspkcpakas kedslpdien
      481 lltelelvag rdprcsvdds lleml
//
LOCUS       XP_077500607             319 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111251 [Amblyomma americanum].
ACCESSION   XP_077500607
VERSION     XP_077500607.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644481.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..319
                     /product="uncharacterized protein LOC144111251"
                     /calculated_mol_wt=35101
     CDS             1..319
                     /gene="LOC144111251"
                     /coded_by="XM_077644481.1:4877..5836"
                     /db_xref="GeneID:144111251"
ORIGIN      
        1 mpkrkckfte qmgkeysfik ktkseadvrc dacgadfsva hggrsdivkh issvkhkqsv
       61 saaarsnavt qffrqerage kelqlaaskg vfafhtiahn hsfrsmdcts kllqklfnek
      121 sscartkaea ialnvlapsa eeelktdlqe akfisvfcdp snhkdlkvlp imvryftaaa
      181 gvqtkllein tlpgktsamv sqyimdalea nqvtsklvaf sadnancnfg gaarngknnv
      241 fsrsqdsmdh kivgigcaah iahntvqtaa dclpldieav ivkiysyfyi ytvimesfkk
      301 fcdfasaeyq rllgysktr
//
LOCUS       XP_077500610             319 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111251 [Amblyomma americanum].
ACCESSION   XP_077500610
VERSION     XP_077500610.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644484.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..319
                     /product="uncharacterized protein LOC144111251"
                     /calculated_mol_wt=35101
     CDS             1..319
                     /gene="LOC144111251"
                     /coded_by="XM_077644484.1:4790..5749"
                     /db_xref="GeneID:144111251"
ORIGIN      
        1 mpkrkckfte qmgkeysfik ktkseadvrc dacgadfsva hggrsdivkh issvkhkqsv
       61 saaarsnavt qffrqerage kelqlaaskg vfafhtiahn hsfrsmdcts kllqklfnek
      121 sscartkaea ialnvlapsa eeelktdlqe akfisvfcdp snhkdlkvlp imvryftaaa
      181 gvqtkllein tlpgktsamv sqyimdalea nqvtsklvaf sadnancnfg gaarngknnv
      241 fsrsqdsmdh kivgigcaah iahntvqtaa dclpldieav ivkiysyfyi ytvimesfkk
      301 fcdfasaeyq rllgysktr
//
LOCUS       XP_077500617             319 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111251 [Amblyomma americanum].
ACCESSION   XP_077500617
VERSION     XP_077500617.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644491.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..319
                     /product="uncharacterized protein LOC144111251"
                     /calculated_mol_wt=35101
     CDS             1..319
                     /gene="LOC144111251"
                     /coded_by="XM_077644491.1:4766..5725"
                     /db_xref="GeneID:144111251"
ORIGIN      
        1 mpkrkckfte qmgkeysfik ktkseadvrc dacgadfsva hggrsdivkh issvkhkqsv
       61 saaarsnavt qffrqerage kelqlaaskg vfafhtiahn hsfrsmdcts kllqklfnek
      121 sscartkaea ialnvlapsa eeelktdlqe akfisvfcdp snhkdlkvlp imvryftaaa
      181 gvqtkllein tlpgktsamv sqyimdalea nqvtsklvaf sadnancnfg gaarngknnv
      241 fsrsqdsmdh kivgigcaah iahntvqtaa dclpldieav ivkiysyfyi ytvimesfkk
      301 fcdfasaeyq rllgysktr
//
LOCUS       XP_077500626             319 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111251 [Amblyomma americanum].
ACCESSION   XP_077500626
VERSION     XP_077500626.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644500.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..319
                     /product="uncharacterized protein LOC144111251"
                     /calculated_mol_wt=35101
     CDS             1..319
                     /gene="LOC144111251"
                     /coded_by="XM_077644500.1:4769..5728"
                     /db_xref="GeneID:144111251"
ORIGIN      
        1 mpkrkckfte qmgkeysfik ktkseadvrc dacgadfsva hggrsdivkh issvkhkqsv
       61 saaarsnavt qffrqerage kelqlaaskg vfafhtiahn hsfrsmdcts kllqklfnek
      121 sscartkaea ialnvlapsa eeelktdlqe akfisvfcdp snhkdlkvlp imvryftaaa
      181 gvqtkllein tlpgktsamv sqyimdalea nqvtsklvaf sadnancnfg gaarngknnv
      241 fsrsqdsmdh kivgigcaah iahntvqtaa dclpldieav ivkiysyfyi ytvimesfkk
      301 fcdfasaeyq rllgysktr
//
LOCUS       XP_077500638             218 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111274 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077500638
VERSION     XP_077500638.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644512.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..218
                     /product="uncharacterized protein LOC144111274 isoform X1"
                     /calculated_mol_wt=25570
     CDS             1..218
                     /gene="LOC144111274"
                     /coded_by="XM_077644512.1:353..1009"
                     /db_xref="GeneID:144111274"
ORIGIN      
        1 menlttstvh sqdrlrhprp mrrrrresth cssdntcycr tceadaaetd rhfnwvtwcp
       61 mylllaaphf lgclpinddg rvkeslehgd dpsethrrlr tssncwhhws syqlsmrhqm
      121 aqkcschwrt aclkeaeeta shklwpaphl qcqccstcta nhrqpqrrhk qgqcwrkqqc
      181 hsrgkqqcrc whkrqkkgtp kelpqatric yraascpp
//
LOCUS       XP_077500645             218 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111274 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077500645
VERSION     XP_077500645.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644519.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..218
                     /product="uncharacterized protein LOC144111274 isoform X1"
                     /calculated_mol_wt=25570
     CDS             1..218
                     /gene="LOC144111274"
                     /coded_by="XM_077644519.1:1006..1662"
                     /db_xref="GeneID:144111274"
ORIGIN      
        1 menlttstvh sqdrlrhprp mrrrrresth cssdntcycr tceadaaetd rhfnwvtwcp
       61 mylllaaphf lgclpinddg rvkeslehgd dpsethrrlr tssncwhhws syqlsmrhqm
      121 aqkcschwrt aclkeaeeta shklwpaphl qcqccstcta nhrqpqrrhk qgqcwrkqqc
      181 hsrgkqqcrc whkrqkkgtp kelpqatric yraascpp
//
LOCUS       XP_077500653             187 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111274 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077500653
VERSION     XP_077500653.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644527.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="uncharacterized protein LOC144111274 isoform X2"
                     /calculated_mol_wt=20318
     CDS             1..187
                     /gene="LOC144111274"
                     /coded_by="XM_077644527.1:348..911"
                     /db_xref="GeneID:144111274"
ORIGIN      
        1 menlttstvh sqdrlrhprp mrrrrresth cssdntcycr tceadaaetd rhfnwvtwcp
       61 mylllaaphf lgclpinddg rvkeslelpv vneapdgsev llsledslpe gsrrdglsqi
      121 vasppfavpv lqylycksqa taaatqagpv laqaavpqqg qaavpvlaqa pkegnpkraa
      181 tsdkdll
//
LOCUS       XP_077500662             143 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111274 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077500662
VERSION     XP_077500662.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644536.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..143
                     /product="uncharacterized protein LOC144111274 isoform X3"
                     /calculated_mol_wt=16699
     CDS             1..143
                     /gene="LOC144111274"
                     /coded_by="XM_077644536.1:359..790"
                     /db_xref="GeneID:144111274"
ORIGIN      
        1 menlttstvh sqdrlrhprp mrrrrresth cssdntcycr tceadaaetd rhfnwvtwcp
       61 mylllaaphf lgclpinddg rvkeslehgd dpsethrrlr tssncwhhws rgsihcslyc
      121 krifqafkke plnrradkat scq
//
LOCUS       XP_077500673             412 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111295 [Amblyomma americanum].
ACCESSION   XP_077500673
VERSION     XP_077500673.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644547.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..412
                     /product="uncharacterized protein LOC144111295"
                     /calculated_mol_wt=46249
     CDS             1..412
                     /gene="LOC144111295"
                     /coded_by="XM_077644547.1:874..2112"
                     /db_xref="GeneID:144111295"
ORIGIN      
        1 mrafqkilya ktmeelqtak delhchkhea fikrvetflq hqqewvllyr agtvtrghnm
       61 nnyseasiri lkdivlcrtk aynavalvef tittwekyfe arllrhahnr dsshrvafqh
      121 llqklpndfa ksivqvdgal ysvpsssgts syevdadigf cscwrgsqga fckhqaavqk
      181 afggcfpnsp eltpedrrrl gylalgdrcp niefflpmmp ggneevtvac tsahseqamh
      241 advsqlpesp pgpfperkek pedmeekyca lekelrrvhk laedsavypk lldavtaqlk
      301 nvttssgaha lllklkassa adrrrgqiir vqqqawqgag layqgarnvf llgghsqqdk
      361 qeksnvatnw avafrkmyrg qkvtrpafig kdeqdaaaqe ynhcycilva sl
//
LOCUS       XP_077500686             575 aa            linear   INV 25-NOV-2025
DEFINITION  membrane palmitoylated protein 7-like protein metro isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077500686
VERSION     XP_077500686.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644560.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..575
                     /product="membrane palmitoylated protein 7-like protein
                     metro isoform X1"
                     /calculated_mol_wt=64336
     Region          17..>59
                     /region_name="L27"
                     /note="domain in receptor targeting proteins Lin-2 and
                     Lin-7; smart00569"
                     /db_xref="CDD:197794"
     Region          81..124
                     /region_name="L27"
                     /note="L27 domain; pfam02828"
                     /db_xref="CDD:460717"
     Region          139..221
                     /region_name="PDZ_MPP3-MPP4-MPP7-like"
                     /note="PDZ domain of membrane palmitoylated proteins 3
                     (MPP3), MPP4, and MPP7, and related domains; cd06799"
                     /db_xref="CDD:467260"
     Site            order(149..155,166,169,199,203,206..207)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467260"
     Region          234..293
                     /region_name="SH3_MPP"
                     /note="Src Homology 3 domain of Membrane Protein,
                     Palmitoylated (or MAGUK p55 subfamily member) proteins;
                     cd11862"
                     /db_xref="CDD:212796"
     Site            order(236,260..261)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212796"
     Site            order(239,241,244,255,273..274,291,293)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212796"
     Region          367..558
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..575
                     /gene="metro"
                     /coded_by="XM_077644560.1:353..2080"
                     /db_xref="GeneID:144111302"
ORIGIN      
        1 mavggkyngt hndqalqrll asleqvcqka hsgdddrafl aallqnkalq alvhihskiv
       61 sfsnqgiaps scdasltaek vlhalqkcte peakelcali ssphvkalfe ahdviakkef
      121 lpslteaiye aeedeetiki vqlvksnepl gatikyneia kaitiarimh ggaadrsgli
      181 hvgdevhevn gvslkgkaph dvvnileqqs qagtitlklv paeeaavgre srvrvralfd
      241 ydpaadplip cqeaglqfrr gqvlhlvsqe dpnwwqarwe gqrsaragli paqslqerrf
      301 aslrvtnqed ddeglsagcy spmrkelkar rakkimydvs dnedfdreli styeevakly
      361 prpglhrpiv ligppgvgrn elkrrlvasd pdkfkttipy tsrpmkpwei dgrdyyfvsr
      421 aemeekirqg rfvehgeyrg nlygtsldti raivdmgytc vlnphpqalk mlrtaelkpf
      481 vifikppsie ilketrnkac arstfdencs rgftddefhd ivylghkmdf lyghmfdlti
      541 vnedlprafy qlmaavkave aepqwvpasw vvtsq
//
LOCUS       XP_077500695             575 aa            linear   INV 25-NOV-2025
DEFINITION  membrane palmitoylated protein 7-like protein metro isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077500695
VERSION     XP_077500695.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644569.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..575
                     /product="membrane palmitoylated protein 7-like protein
                     metro isoform X1"
                     /calculated_mol_wt=64336
     Region          17..>59
                     /region_name="L27"
                     /note="domain in receptor targeting proteins Lin-2 and
                     Lin-7; smart00569"
                     /db_xref="CDD:197794"
     Region          81..124
                     /region_name="L27"
                     /note="L27 domain; pfam02828"
                     /db_xref="CDD:460717"
     Region          139..221
                     /region_name="PDZ_MPP3-MPP4-MPP7-like"
                     /note="PDZ domain of membrane palmitoylated proteins 3
                     (MPP3), MPP4, and MPP7, and related domains; cd06799"
                     /db_xref="CDD:467260"
     Site            order(149..155,166,169,199,203,206..207)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467260"
     Region          234..293
                     /region_name="SH3_MPP"
                     /note="Src Homology 3 domain of Membrane Protein,
                     Palmitoylated (or MAGUK p55 subfamily member) proteins;
                     cd11862"
                     /db_xref="CDD:212796"
     Site            order(236,260..261)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212796"
     Site            order(239,241,244,255,273..274,291,293)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212796"
     Region          367..558
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..575
                     /gene="metro"
                     /coded_by="XM_077644569.1:323..2050"
                     /db_xref="GeneID:144111302"
ORIGIN      
        1 mavggkyngt hndqalqrll asleqvcqka hsgdddrafl aallqnkalq alvhihskiv
       61 sfsnqgiaps scdasltaek vlhalqkcte peakelcali ssphvkalfe ahdviakkef
      121 lpslteaiye aeedeetiki vqlvksnepl gatikyneia kaitiarimh ggaadrsgli
      181 hvgdevhevn gvslkgkaph dvvnileqqs qagtitlklv paeeaavgre srvrvralfd
      241 ydpaadplip cqeaglqfrr gqvlhlvsqe dpnwwqarwe gqrsaragli paqslqerrf
      301 aslrvtnqed ddeglsagcy spmrkelkar rakkimydvs dnedfdreli styeevakly
      361 prpglhrpiv ligppgvgrn elkrrlvasd pdkfkttipy tsrpmkpwei dgrdyyfvsr
      421 aemeekirqg rfvehgeyrg nlygtsldti raivdmgytc vlnphpqalk mlrtaelkpf
      481 vifikppsie ilketrnkac arstfdencs rgftddefhd ivylghkmdf lyghmfdlti
      541 vnedlprafy qlmaavkave aepqwvpasw vvtsq
//
LOCUS       XP_077500701             574 aa            linear   INV 25-NOV-2025
DEFINITION  membrane palmitoylated protein 7-like protein metro isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077500701
VERSION     XP_077500701.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644575.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..574
                     /product="membrane palmitoylated protein 7-like protein
                     metro isoform X2"
                     /calculated_mol_wt=64206
     Region          17..>59
                     /region_name="L27"
                     /note="domain in receptor targeting proteins Lin-2 and
                     Lin-7; smart00569"
                     /db_xref="CDD:197794"
     Region          81..124
                     /region_name="L27"
                     /note="L27 domain; pfam02828"
                     /db_xref="CDD:460717"
     Region          139..220
                     /region_name="PDZ_MPP3-MPP4-MPP7-like"
                     /note="PDZ domain of membrane palmitoylated proteins 3
                     (MPP3), MPP4, and MPP7, and related domains; cd06799"
                     /db_xref="CDD:467260"
     Site            order(149..155,166,169,199,203,206..207)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467260"
     Region          233..292
                     /region_name="SH3_MPP"
                     /note="Src Homology 3 domain of Membrane Protein,
                     Palmitoylated (or MAGUK p55 subfamily member) proteins;
                     cd11862"
                     /db_xref="CDD:212796"
     Site            order(235,259..260)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212796"
     Site            order(238,240,243,254,272..273,290,292)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212796"
     Region          366..557
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..574
                     /gene="metro"
                     /coded_by="XM_077644575.1:546..2270"
                     /db_xref="GeneID:144111302"
ORIGIN      
        1 mavggkyngt hndqalqrll asleqvcqka hsgdddrafl aallqnkalq alvhihskiv
       61 sfsnqgiaps scdasltaek vlhalqkcte peakelcali ssphvkalfe ahdviakkef
      121 lpslteaiye aeedeetiki vqlvksnepl gatikyneia kaitiarimh ggaadrsgli
      181 hvgdevhevn gvslkgkaph dvvnilqqsq agtitlklvp aeeaavgres rvrvralfdy
      241 dpaadplipc qeaglqfrrg qvlhlvsqed pnwwqarweg qrsaraglip aqslqerrfa
      301 slrvtnqedd deglsagcys pmrkelkarr akkimydvsd nedfdrelis tyeevaklyp
      361 rpglhrpivl igppgvgrne lkrrlvasdp dkfkttipyt srpmkpweid grdyyfvsra
      421 emeekirqgr fvehgeyrgn lygtsldtir aivdmgytcv lnphpqalkm lrtaelkpfv
      481 ifikppsiei lketrnkaca rstfdencsr gftddefhdi vylghkmdfl yghmfdltiv
      541 nedlprafyq lmaavkavea epqwvpaswv vtsq
//
LOCUS       XP_077500708             574 aa            linear   INV 25-NOV-2025
DEFINITION  membrane palmitoylated protein 7-like protein metro isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077500708
VERSION     XP_077500708.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644582.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..574
                     /product="membrane palmitoylated protein 7-like protein
                     metro isoform X2"
                     /calculated_mol_wt=64206
     Region          17..>59
                     /region_name="L27"
                     /note="domain in receptor targeting proteins Lin-2 and
                     Lin-7; smart00569"
                     /db_xref="CDD:197794"
     Region          81..124
                     /region_name="L27"
                     /note="L27 domain; pfam02828"
                     /db_xref="CDD:460717"
     Region          139..220
                     /region_name="PDZ_MPP3-MPP4-MPP7-like"
                     /note="PDZ domain of membrane palmitoylated proteins 3
                     (MPP3), MPP4, and MPP7, and related domains; cd06799"
                     /db_xref="CDD:467260"
     Site            order(149..155,166,169,199,203,206..207)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467260"
     Region          233..292
                     /region_name="SH3_MPP"
                     /note="Src Homology 3 domain of Membrane Protein,
                     Palmitoylated (or MAGUK p55 subfamily member) proteins;
                     cd11862"
                     /db_xref="CDD:212796"
     Site            order(235,259..260)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212796"
     Site            order(238,240,243,254,272..273,290,292)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212796"
     Region          366..557
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..574
                     /gene="metro"
                     /coded_by="XM_077644582.1:281..2005"
                     /db_xref="GeneID:144111302"
ORIGIN      
        1 mavggkyngt hndqalqrll asleqvcqka hsgdddrafl aallqnkalq alvhihskiv
       61 sfsnqgiaps scdasltaek vlhalqkcte peakelcali ssphvkalfe ahdviakkef
      121 lpslteaiye aeedeetiki vqlvksnepl gatikyneia kaitiarimh ggaadrsgli
      181 hvgdevhevn gvslkgkaph dvvnilqqsq agtitlklvp aeeaavgres rvrvralfdy
      241 dpaadplipc qeaglqfrrg qvlhlvsqed pnwwqarweg qrsaraglip aqslqerrfa
      301 slrvtnqedd deglsagcys pmrkelkarr akkimydvsd nedfdrelis tyeevaklyp
      361 rpglhrpivl igppgvgrne lkrrlvasdp dkfkttipyt srpmkpweid grdyyfvsra
      421 emeekirqgr fvehgeyrgn lygtsldtir aivdmgytcv lnphpqalkm lrtaelkpfv
      481 ifikppsiei lketrnkaca rstfdencsr gftddefhdi vylghkmdfl yghmfdltiv
      541 nedlprafyq lmaavkavea epqwvpaswv vtsq
//
LOCUS       XP_077500715             574 aa            linear   INV 25-NOV-2025
DEFINITION  membrane palmitoylated protein 7-like protein metro isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077500715
VERSION     XP_077500715.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644589.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..574
                     /product="membrane palmitoylated protein 7-like protein
                     metro isoform X2"
                     /calculated_mol_wt=64206
     Region          17..>59
                     /region_name="L27"
                     /note="domain in receptor targeting proteins Lin-2 and
                     Lin-7; smart00569"
                     /db_xref="CDD:197794"
     Region          81..124
                     /region_name="L27"
                     /note="L27 domain; pfam02828"
                     /db_xref="CDD:460717"
     Region          139..220
                     /region_name="PDZ_MPP3-MPP4-MPP7-like"
                     /note="PDZ domain of membrane palmitoylated proteins 3
                     (MPP3), MPP4, and MPP7, and related domains; cd06799"
                     /db_xref="CDD:467260"
     Site            order(149..155,166,169,199,203,206..207)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467260"
     Region          233..292
                     /region_name="SH3_MPP"
                     /note="Src Homology 3 domain of Membrane Protein,
                     Palmitoylated (or MAGUK p55 subfamily member) proteins;
                     cd11862"
                     /db_xref="CDD:212796"
     Site            order(235,259..260)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212796"
     Site            order(238,240,243,254,272..273,290,292)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212796"
     Region          366..557
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..574
                     /gene="metro"
                     /coded_by="XM_077644589.1:411..2135"
                     /db_xref="GeneID:144111302"
ORIGIN      
        1 mavggkyngt hndqalqrll asleqvcqka hsgdddrafl aallqnkalq alvhihskiv
       61 sfsnqgiaps scdasltaek vlhalqkcte peakelcali ssphvkalfe ahdviakkef
      121 lpslteaiye aeedeetiki vqlvksnepl gatikyneia kaitiarimh ggaadrsgli
      181 hvgdevhevn gvslkgkaph dvvnilqqsq agtitlklvp aeeaavgres rvrvralfdy
      241 dpaadplipc qeaglqfrrg qvlhlvsqed pnwwqarweg qrsaraglip aqslqerrfa
      301 slrvtnqedd deglsagcys pmrkelkarr akkimydvsd nedfdrelis tyeevaklyp
      361 rpglhrpivl igppgvgrne lkrrlvasdp dkfkttipyt srpmkpweid grdyyfvsra
      421 emeekirqgr fvehgeyrgn lygtsldtir aivdmgytcv lnphpqalkm lrtaelkpfv
      481 ifikppsiei lketrnkaca rstfdencsr gftddefhdi vylghkmdfl yghmfdltiv
      541 nedlprafyq lmaavkavea epqwvpaswv vtsq
//
LOCUS       XP_077500722             573 aa            linear   INV 25-NOV-2025
DEFINITION  membrane palmitoylated protein 7-like protein metro isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077500722
VERSION     XP_077500722.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644596.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..573
                     /product="membrane palmitoylated protein 7-like protein
                     metro isoform X3"
                     /calculated_mol_wt=64078
     Region          17..>59
                     /region_name="L27"
                     /note="domain in receptor targeting proteins Lin-2 and
                     Lin-7; smart00569"
                     /db_xref="CDD:197794"
     Region          81..124
                     /region_name="L27"
                     /note="L27 domain; pfam02828"
                     /db_xref="CDD:460717"
     Region          139..219
                     /region_name="PDZ_MPP3-MPP4-MPP7-like"
                     /note="PDZ domain of membrane palmitoylated proteins 3
                     (MPP3), MPP4, and MPP7, and related domains; cd06799"
                     /db_xref="CDD:467260"
     Site            order(149..155,166,169,199,203,206..207)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467260"
     Region          232..291
                     /region_name="SH3_MPP"
                     /note="Src Homology 3 domain of Membrane Protein,
                     Palmitoylated (or MAGUK p55 subfamily member) proteins;
                     cd11862"
                     /db_xref="CDD:212796"
     Site            order(234,258..259)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212796"
     Site            order(237,239,242,253,271..272,289,291)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212796"
     Region          365..556
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..573
                     /gene="metro"
                     /coded_by="XM_077644596.1:546..2267"
                     /db_xref="GeneID:144111302"
ORIGIN      
        1 mavggkyngt hndqalqrll asleqvcqka hsgdddrafl aallqnkalq alvhihskiv
       61 sfsnqgiaps scdasltaek vlhalqkcte peakelcali ssphvkalfe ahdviakkef
      121 lpslteaiye aeedeetiki vqlvksnepl gatikyneia kaitiarimh ggaadrsgli
      181 hvgdevhevn gvslkgkaph dvvnilqsqa gtitlklvpa eeaavgresr vrvralfdyd
      241 paadplipcq eaglqfrrgq vlhlvsqedp nwwqarwegq rsaraglipa qslqerrfas
      301 lrvtnqeddd eglsagcysp mrkelkarra kkimydvsdn edfdrelist yeevaklypr
      361 pglhrpivli gppgvgrnel krrlvasdpd kfkttipyts rpmkpweidg rdyyfvsrae
      421 meekirqgrf vehgeyrgnl ygtsldtira ivdmgytcvl nphpqalkml rtaelkpfvi
      481 fikppsieil ketrnkacar stfdencsrg ftddefhdiv ylghkmdfly ghmfdltivn
      541 edlprafyql maavkaveae pqwvpaswvv tsq
//
LOCUS       XP_077500731             573 aa            linear   INV 25-NOV-2025
DEFINITION  membrane palmitoylated protein 7-like protein metro isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077500731
VERSION     XP_077500731.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644605.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..573
                     /product="membrane palmitoylated protein 7-like protein
                     metro isoform X3"
                     /calculated_mol_wt=64078
     Region          17..>59
                     /region_name="L27"
                     /note="domain in receptor targeting proteins Lin-2 and
                     Lin-7; smart00569"
                     /db_xref="CDD:197794"
     Region          81..124
                     /region_name="L27"
                     /note="L27 domain; pfam02828"
                     /db_xref="CDD:460717"
     Region          139..219
                     /region_name="PDZ_MPP3-MPP4-MPP7-like"
                     /note="PDZ domain of membrane palmitoylated proteins 3
                     (MPP3), MPP4, and MPP7, and related domains; cd06799"
                     /db_xref="CDD:467260"
     Site            order(149..155,166,169,199,203,206..207)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467260"
     Region          232..291
                     /region_name="SH3_MPP"
                     /note="Src Homology 3 domain of Membrane Protein,
                     Palmitoylated (or MAGUK p55 subfamily member) proteins;
                     cd11862"
                     /db_xref="CDD:212796"
     Site            order(234,258..259)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212796"
     Site            order(237,239,242,253,271..272,289,291)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212796"
     Region          365..556
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..573
                     /gene="metro"
                     /coded_by="XM_077644605.1:379..2100"
                     /db_xref="GeneID:144111302"
ORIGIN      
        1 mavggkyngt hndqalqrll asleqvcqka hsgdddrafl aallqnkalq alvhihskiv
       61 sfsnqgiaps scdasltaek vlhalqkcte peakelcali ssphvkalfe ahdviakkef
      121 lpslteaiye aeedeetiki vqlvksnepl gatikyneia kaitiarimh ggaadrsgli
      181 hvgdevhevn gvslkgkaph dvvnilqsqa gtitlklvpa eeaavgresr vrvralfdyd
      241 paadplipcq eaglqfrrgq vlhlvsqedp nwwqarwegq rsaraglipa qslqerrfas
      301 lrvtnqeddd eglsagcysp mrkelkarra kkimydvsdn edfdrelist yeevaklypr
      361 pglhrpivli gppgvgrnel krrlvasdpd kfkttipyts rpmkpweidg rdyyfvsrae
      421 meekirqgrf vehgeyrgnl ygtsldtira ivdmgytcvl nphpqalkml rtaelkpfvi
      481 fikppsieil ketrnkacar stfdencsrg ftddefhdiv ylghkmdfly ghmfdltivn
      541 edlprafyql maavkaveae pqwvpaswvv tsq
//
LOCUS       XP_077500740             336 aa            linear   INV 25-NOV-2025
DEFINITION  trypsin-1-like [Amblyomma americanum].
ACCESSION   XP_077500740
VERSION     XP_077500740.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644614.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..336
                     /product="trypsin-1-like"
                     /calculated_mol_wt=36131
     Region          68..300
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cd00190"
                     /db_xref="CDD:238113"
     Site            68
                     /site_type="active"
                     /note="cleavage site [active]"
                     /db_xref="CDD:238113"
     Site            order(113,162,254)
                     /site_type="active"
                     /db_xref="CDD:238113"
     Site            order(248,273,275)
                     /site_type="other"
                     /note="substrate binding sites [chemical binding]"
                     /db_xref="CDD:238113"
     CDS             1..336
                     /gene="LOC144111340"
                     /coded_by="XM_077644614.1:292..1302"
                     /db_xref="GeneID:144111340"
ORIGIN      
        1 mprpaaarag asgllvllly aataadpaag avpplskaev defwkiynad erplcpsaas
       61 eqhrsrrvvg grdagepvpf maslqknvtg lskpfpfcga smlterhlvt aahclknkta
      121 smlsvnvgdy nlsatddann ivrnllsflq henyiiptyd ndiavltlid pvpweslttv
      181 vlppvnsdla qdttvcvmgw grlqyegakp ttlqklcmps vnrtecqrpl thqvhenmic
      241 aggregqdac vgdsggplmv qagnyeaivg vvsfgkkcal kdvygvytrv grfvpwlyeh
      301 trnatckpti saeadfpdie tttkpalpep vadnhi
//
LOCUS       XP_077500755             863 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111354 [Amblyomma americanum].
ACCESSION   XP_077500755
VERSION     XP_077500755.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644629.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..863
                     /product="uncharacterized protein LOC144111354"
                     /calculated_mol_wt=97318
     Region          4..79
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          173..404
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..863
                     /gene="LOC144111354"
                     /coded_by="XM_077644629.1:170..2761"
                     /db_xref="GeneID:144111354"
ORIGIN      
        1 mpgccvplcs nhsrngwrmf sfprdpkrrl lwtvkikrdk wqpanssric nahfedncye
       61 nhradgrkll ksnavptifs frpmvhhqrk apkergtpal pttsnstvqe qgpgvfadcd
      121 epggtnlsee vvyqpsdgqt eqnegcdahs sssccaqlrk qledvrknhr qlqevhkkan
      181 ltinslrkkv rklksavehf kkrikflnkd qvralsrksn kgsiwspqti kqglqlkfsc
      241 gksgyrtlrq lgyplpaert lsrrlhwlkf spgiltdvve llqakaqgme dvekdcvlfi
      301 demeiargfq ldraedivyg gvtlpqkpde panhalvfmv gglnqrwkqv iayhftgshv
      361 dgrtlkdivl qvvqlcadis lrvrvvtsdm gasnramwre lgfsshrnsk ttcsiphpsl
      421 ndmelffnad aahvlknikg qlitshaftl seatasqcdl psrevkldhv havlefdker
      481 elklapklse ahvssghftk mkvglavqff reapsairyl ikekvldiea ettawflelv
      541 srwyalmssr hpsvalslhd vakhhralet lnlalntfqg msmgstsqwk psqagllist
      601 tvvlrlqdil lnsegysfll tsrllqdcle nlfsvvrlrk pvpdaydmkc alklvcisqf
      661 lhtpsttsys vddseylvdl lsngkrelae aevkeiddse vlfiealtst ecsilfhiag
      721 flvkgilkai nnceqckatl lgssdaenay ltrlkeylen gdnlhypspe vmkvlkscee
      781 hfkgitewae slltmkspvq avitylrqmv pfnvetcqvh neiiqkllys tyartrlrih
      841 lrqleekkvn ghasktcagv slp
//
LOCUS       XP_077500768             226 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111362 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077500768
VERSION     XP_077500768.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644642.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..226
                     /product="uncharacterized protein LOC144111362 isoform X1"
                     /calculated_mol_wt=25422
     CDS             1..226
                     /gene="LOC144111362"
                     /coded_by="XM_077644642.1:94..774"
                     /db_xref="GeneID:144111362"
ORIGIN      
        1 msrsadcsse aetrplmraa spvgclsavr lrsvserrpr sqamlqpdsm naetaslpgs
       61 rrnpldvfts mfrrrslrpt ggpsmtqssd riwdllstsp vrvrnleqdm ealrqsrhdn
      121 pylqqqmerf slqdepader qllfppddfr dlhkhlirsp ppqfpqdvah fvfpgspppe
      181 ddammepemv grsfaepahe vppeshdqka ypddvhrglp aaattr
//
LOCUS       XP_077500776             203 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111362 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077500776
VERSION     XP_077500776.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644650.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..203
                     /product="uncharacterized protein LOC144111362 isoform X2"
                     /calculated_mol_wt=22770
     CDS             1..203
                     /gene="LOC144111362"
                     /coded_by="XM_077644650.1:113..724"
                     /db_xref="GeneID:144111362"
ORIGIN      
        1 mheastsdgr ralpsasapa mlqpdsmnae taslpgsrrn pldvftsmfr rrslrptggp
       61 smtqssdriw dllstspvrv rnleqdmeal rqsrhdnpyl qqqmerfslq depaderqll
      121 fppddfrdlh khlirspppq fpqdvahfvf pgspppedda mmepemvgrs faepahevpp
      181 eshdqkaypd dvhrglpaaa ttr
//
LOCUS       XP_077500781             183 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111362 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077500781
VERSION     XP_077500781.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644655.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="uncharacterized protein LOC144111362 isoform X3"
                     /calculated_mol_wt=20776
     CDS             1..183
                     /gene="LOC144111362"
                     /coded_by="XM_077644655.1:107..658"
                     /db_xref="GeneID:144111362"
ORIGIN      
        1 mlqpdsmnae taslpgsrrn pldvftsmfr rrslrptggp smtqssdriw dllstspvrv
       61 rnleqdmeal rqsrhdnpyl qqqmerfslq depaderqll fppddfrdlh khlirspppq
      121 fpqdvahfvf pgspppedda mmepemvgrs faepahevpp eshdqkaypd dvhrglpaaa
      181 ttr
//
LOCUS       XP_077500790             117 aa            linear   INV 25-NOV-2025
DEFINITION  retinal homeobox protein Rx3-like [Amblyomma americanum].
ACCESSION   XP_077500790
VERSION     XP_077500790.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644664.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..117
                     /product="retinal homeobox protein Rx3-like"
                     /calculated_mol_wt=12944
     Region          58..106
                     /region_name="homeodomain"
                     /note="Homeodomain; DNA binding domains involved in the
                     transcriptional regulation of key eukaryotic developmental
                     processes; may bind to DNA as monomers or as homo- and/or
                     heterodimers, in a sequence-specific manner; cd00086"
                     /db_xref="CDD:238039"
     CDS             1..117
                     /gene="LOC144111380"
                     /coded_by="XM_077644664.1:98..451"
                     /db_xref="GeneID:144111380"
ORIGIN      
        1 mflgrgsptv kaaggdprad saalekdwsp rsarrmastt iqahlqpshr lpmvlgfqer
       61 eleaafehnr npnfsdllil aaevglplda vtvwfenrla rwrvsqglpa nrrmvnq
//
LOCUS       XP_077500801            1070 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC144111390
            [Amblyomma americanum].
ACCESSION   XP_077500801
VERSION     XP_077500801.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644675.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1070
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC144111390"
                     /calculated_mol_wt=118575
     Region          37..130
                     /region_name="COMPASS-Shg1"
                     /note="COMPASS (Complex proteins associated with Set1p)
                     component shg1; pfam05205"
                     /db_xref="CDD:461586"
     Region          <304..>541
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          461..>662
                     /region_name="PRK08581"
                     /note="amidase domain-containing protein"
                     /db_xref="CDD:236304"
     CDS             1..1070
                     /gene="LOC144111390"
                     /coded_by="XM_077644675.1:135..3347"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 1 base in 1 codon"
                     /db_xref="GeneID:144111390"
ORIGIN      
        1 mhaytsdlae mplgatmtsn ssirelppgd peltanivsh lksrgxfdkl rrdclgdvet
       61 kpaylnlkqr iegyitkfls tqtwtpdmnk nhvrdmmrre inesgmlsag mdhlvdqivn
      121 pkiyqvftpe vedgvlqylg lssandtsth qpapeaaadt tsqpppqtpv lpppplppks
      181 efsidettqd vpgscspkpy ieafmpddft dsssephlmi vtdncertee lpaelqfeda
      241 ssadlcdtsa eplqpipnei vgeapvpeqv damepepqvi cpsvnadgrl paedvvvesa
      301 vplddmksdf mserkpieid kkdgekiadl kkkeskcvdk drkerkldsg kgrelgshss
      361 rseshskihm skkgsdgsrs kphksrgdgd lskesrdlsk ekkrsssrdk gsssrgemsr
      421 nktdvskder gkgysgqssr skecdirser krsddrhrhy sssqphddkk rhsdrhsree
      481 ksrdrsdshr shnesrrdkd ksstkvssrq dkpskssekh gessqkdkgh ekerkdysrq
      541 sfsdgrdgga skkasseqtt qpmcqssfqa ndstschvdm pgtfipdrle mrrnshdssq
      601 lsfqsasqds slgivehvtq nalkegilns anqsetksdv qaaediaqet lsetydessr
      661 dalqlveake aeilrfsyvt dkassnsgid lqlvdtvtee ppqdvhskep aqlengdqts
      721 vpgdvalldv peqksllkge khaavrrktt tetnspekkr prredsdghv sdgalsdvtv
      781 ssvhtsdlss yddcisvssa deddcaivre kkkiplreik kmtsssnedd drmsrsetep
      841 pacdgvmplp vvpeevtece ptpnkelqpp speplqtsnt ekasscpstv isggapepkm
      901 glrrtrkinp kyvseefssi ftegkkptgt idfselakyg kerhheeqss lraeprkvfs
      961 regasdhfgt lmsyeetdhl seasvtdrsr rparrlsgne prprsestss kryqssdlyk
     1021 prpvipsrsr rsrpspprqd apkkqqdlkv cigakrgrpy tagssrgkrl
//
LOCUS       XP_077500808             443 aa            linear   INV 25-NOV-2025
DEFINITION  suppressor of fused domain protein isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077500808
VERSION     XP_077500808.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644682.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="suppressor of fused domain protein isoform X1"
                     /calculated_mol_wt=48344
     Region          54..230
                     /region_name="SUFU"
                     /note="Suppressor of fused protein (SUFU); pfam05076"
                     /db_xref="CDD:461543"
     Region          245..438
                     /region_name="SUFU_C"
                     /note="Suppressor of Fused Gli/Ci N terminal binding
                     domain; pfam12470"
                     /db_xref="CDD:463598"
     CDS             1..443
                     /gene="Su(fu)"
                     /coded_by="XM_077644682.1:27..1358"
                     /db_xref="GeneID:144111397"
ORIGIN      
        1 meakrgvgas gfpvvgfpqi ppglealysa clqiysdqpn plqvaavlky wlggpdpldy
       61 ismyanqgde skeipphwhy vsfglsdlhg dgrvheisga dtpsgfgfel tfrlrkepde
      121 tgpptwpaav mqglakyvfq sgnvlcdgdh vswhcpldns esriehmlmm edpqlgtvvt
      181 pfgpvsfiqi vgvcaeelka aqqwngrsfv almqrvpgag gpwlvtnmrr getvfeldps
      241 iqdavdqgie vegsdlsgvs aklcwseefp pvvqsakepg ntasvdtpsi sqyeseeikr
      301 tlkkgllgsq pssetedngl etrelaqtra leavhlqlnl eagllfplvl rgrlkhgrhf
      361 tfkssvdcaa vtfvvpsvig afanaeqpyt vqgswlqill tedlisemtv smeelctaeg
      421 ltlpktytwp qrkmsitllp eel
//
LOCUS       XP_077500816             393 aa            linear   INV 25-NOV-2025
DEFINITION  suppressor of fused domain protein isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077500816
VERSION     XP_077500816.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644690.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..393
                     /product="suppressor of fused domain protein isoform X2"
                     /calculated_mol_wt=43015
     Region          4..180
                     /region_name="SUFU"
                     /note="Suppressor of fused protein (SUFU); pfam05076"
                     /db_xref="CDD:461543"
     Region          195..388
                     /region_name="SUFU_C"
                     /note="Suppressor of Fused Gli/Ci N terminal binding
                     domain; pfam12470"
                     /db_xref="CDD:463598"
     CDS             1..393
                     /gene="Su(fu)"
                     /coded_by="XM_077644690.1:201..1382"
                     /db_xref="GeneID:144111397"
ORIGIN      
        1 mlggpdpldy ismyanqgde skeipphwhy vsfglsdlhg dgrvheisga dtpsgfgfel
       61 tfrlrkepde tgpptwpaav mqglakyvfq sgnvlcdgdh vswhcpldns esriehmlmm
      121 edpqlgtvvt pfgpvsfiqi vgvcaeelka aqqwngrsfv almqrvpgag gpwlvtnmrr
      181 getvfeldps iqdavdqgie vegsdlsgvs aklcwseefp pvvqsakepg ntasvdtpsi
      241 sqyeseeikr tlkkgllgsq pssetedngl etrelaqtra leavhlqlnl eagllfplvl
      301 rgrlkhgrhf tfkssvdcaa vtfvvpsvig afanaeqpyt vqgswlqill tedlisemtv
      361 smeelctaeg ltlpktytwp qrkmsitllp eel
//
LOCUS       XP_077500827             819 aa            linear   INV 25-NOV-2025
DEFINITION  mannosyl-oligosaccharide glucosidase [Amblyomma americanum].
ACCESSION   XP_077500827
VERSION     XP_077500827.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644701.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..819
                     /product="mannosyl-oligosaccharide glucosidase"
                     /calculated_mol_wt=93299
     Region          88..>244
                     /region_name="Glyco_hydro_63N"
                     /note="Glycosyl hydrolase family 63 N-terminal domain;
                     pfam16923"
                     /db_xref="CDD:465316"
     Region          331..817
                     /region_name="Glyco_hydro_63"
                     /note="Glycosyl hydrolase family 63 C-terminal domain;
                     pfam03200"
                     /db_xref="CDD:397353"
     CDS             1..819
                     /gene="GCS1"
                     /coded_by="XM_077644701.1:238..2697"
                     /db_xref="GeneID:144111414"
ORIGIN      
        1 marqrkieaa rpapgrpask pavarvrkas pgpkfdskft ifvvfngcli icvilyvyfl
       61 hyleswvirp vdlpkvvtks gldiperfwg syrsssyfgl ktrsaqplla glmwfthkik
      121 nqnlpirhwc nqwdrlqqyg wlrhdgvnfg tqrivddeyv ietsfvkrag glnggdwtar
      181 iavkpkapga vptapmsllf yfatdghgrl wpnvdakshl eyisgeaqdl gqftatfkdk
      241 ntenvihysy lstvapglen lketvlhnlm tfrrkkgsrd lyvtrgtvls aedmerkrsp
      301 nfvltqvtvm ppyelevife sgsfenrpnt lagenfdqal sehiskfder fdklfnlrsk
      361 gfseedneaa kevlsnmvgg igyfygsslv qsshnkepve ywkaplytav psrsffprgf
      421 lwdegfhnll lmrwdteisk dilahwldlm nvdgwipreq ilgaearsqv psefvvqkdt
      481 nanppalllt leallnqpdv laahgdflqr afprflklfn wlnttqvgkv pgsyrwrgrd
      541 assrtelnpk tltsglddyp rashpsever hvdlhcwlaf ssgvlaraah llndpsshsl
      601 tathqllsng avldkmhlsp rtqrycdygl htdkvrlveq kpppgqapga pplpkvrlam
      661 etprlqyveh fgyvslfpfl lrlltpdnpv lgklltdlhd pdllwtdygl rslsknspmy
      721 kmyntetdpp ywrgaiwini nylavkalnh ysdlpgpfqe kafelykelr snlisnilre
      781 yrrtkyiwen yddrtgeghg shpftgwsal lvlimgevy
//
LOCUS       XP_077500842             376 aa            linear   INV 25-NOV-2025
DEFINITION  actin-related protein 1 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077500842
VERSION     XP_077500842.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644716.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..376
                     /product="actin-related protein 1 isoform X1"
                     /calculated_mol_wt=42479
     Region          9..375
                     /region_name="ASKHA_NBD_Arp1"
                     /note="nucleotide-binding domain (NBD) of actin-related
                     protein 1 (Arp1) and similar proteins; cd10216"
                     /db_xref="CDD:466820"
     CDS             1..376
                     /gene="Arp1"
                     /coded_by="XM_077644716.1:104..1234"
                     /db_xref="GeneID:144111418"
ORIGIN      
        1 metydvivnq pvvidngsgv ikagfagdqv pkchfpnfig rpkhkrvmag alegdifvgp
       61 kaeehrglls irypmehgiv tdwndmeriw qyiyskeqlq tfseehpvll teapinplrn
      121 rersaeiffe tfnvpalfvs mqavlslyat grttgvvlds gdgvthavpi yegfaiphsi
      181 lrvdvagrdv taylrhllrk egtnfhttae feivktvkek acylannpqr eetmdtekah
      241 ytlpdgttle igparfrape vlfrpdlige efeglhqvlv faihkcdmdl rrvlyqnivl
      301 sggstlfkgf gdrllsevkk ispkgikiri sapqerlyst wiggsilasl dtfkrmwvsk
      361 reyeeegmra lhrktf
//
LOCUS       XP_077500850             368 aa            linear   INV 25-NOV-2025
DEFINITION  actin-related protein 1 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077500850
VERSION     XP_077500850.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644724.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..368
                     /product="actin-related protein 1 isoform X2"
                     /calculated_mol_wt=41661
     Region          9..367
                     /region_name="ASKHA_NBD_Arp1"
                     /note="nucleotide-binding domain (NBD) of actin-related
                     protein 1 (Arp1) and similar proteins; cd10216"
                     /db_xref="CDD:466820"
     CDS             1..368
                     /gene="Arp1"
                     /coded_by="XM_077644724.1:86..1192"
                     /db_xref="GeneID:144111418"
ORIGIN      
        1 mirflshqgs gvikagfagd qvpkchfpnf igrpkhkrvm agalegdifv gpkaeehrgl
       61 lsirypmehg ivtdwndmer iwqyiyskeq lqtfseehpv llteapinpl rnrersaeif
      121 fetfnvpalf vsmqavlsly atgrttgvvl dsgdgvthav piyegfaiph silrvdvagr
      181 dvtaylrhll rkegtnfhtt aefeivktvk ekacylannp qreetmdtek ahytlpdgtt
      241 leigparfra pevlfrpdli geefeglhqv lvfaihkcdm dlrrvlyqni vlsggstlfk
      301 gfgdrllsev kkispkgiki risapqerly stwiggsila sldtfkrmwv skreyeeegm
      361 ralhrktf
//
LOCUS       XP_077500859             417 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111431 [Amblyomma americanum].
ACCESSION   XP_077500859
VERSION     XP_077500859.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644733.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..417
                     /product="uncharacterized protein LOC144111431"
                     /calculated_mol_wt=46469
     CDS             1..417
                     /gene="LOC144111431"
                     /coded_by="XM_077644733.1:318..1571"
                     /db_xref="GeneID:144111431"
ORIGIN      
        1 mlsfincsee rdrfqvvpip vpienfeatg vvklwcvrvn hlfyilkgde nlyaccvnts
       61 qsgpfksiki alasslrtll svrsnhvvli fddgslrtlh fvsedgwved mtfklpvdat
      121 ctveyassst sgslywiqrs tqfpdevgys lwkcslspdr delpstqcla hrlpafqlyv
      181 lrhaviiips lpdppnvylv fgsahdlkmg slakqavllt ttlsspldva tfykrnirfw
      241 qmqthatlvl hgcvsplasc lylllkdstv vmlsssgevl wrvtlqevpp himgcsvmas
      301 tlclfddsav qlydltegtl leeltlqgtf cgvlpslwaf wttngvyrln vsgntwyrns
      361 pgqlqsealv yaawhagaei vprfptdaqv rtklnrilhp lvecycklqq tkvalts
//
LOCUS       XP_077500869             416 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111441 [Amblyomma americanum].
ACCESSION   XP_077500869
VERSION     XP_077500869.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644743.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (DAPXPI010001813.1) annotated
            using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 703 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..416
                     /product="uncharacterized protein LOC144111441"
                     /calculated_mol_wt=45894
     Region          <259..343
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          272..276
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          311..315
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          325..330
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          338..341
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          354..>390
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          369..373
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          383..387
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..416
                     /gene="LOC144111441"
                     /coded_by="XM_077644743.1:207..1457"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 703 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144111441"
ORIGIN      
        1 migasirhav liclairtvs glridgpdlv lqgdslwlnc sddhgtapsa llwykdnmkf
       61 ysydrqaepp srsyrvpgvh vdlgqssegf vrlvdadirt rgvyrcdlql epgvvpehkq
      121 lqvrvvaadl qgptlggika ryrigerina tcssgpsseg ptlrwlindr epddeeteqr
      181 velvrvgale errqqlalra rdqhfvdgvm riscvmdvpv lgrrvaeklv trattpplrp
      241 enqeegvvls vpsvvirdsg fwlncsspga hsiqwlkdgq efyvysstss tpkiigsvsg
      301 vhinedisyr ghiylsrsel ttegtytckv klpsyrtkis ekklqvyalp srdpeienlq
      361 taykigdain atcssgpskp apiltwyvng drvdenvtdr aaiptgkgtf yavgqa
//
LOCUS       XP_077500882             142 aa            linear   INV 25-NOV-2025
DEFINITION  golgin subfamily A member 7 [Amblyomma americanum].
ACCESSION   XP_077500882
VERSION     XP_077500882.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644756.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..142
                     /product="golgin subfamily A member 7"
                     /calculated_mol_wt=16048
     Region          18..131
                     /region_name="Erf4"
                     /note="Golgin subfamily A member 7/ERF4 family; pfam10256"
                     /db_xref="CDD:463029"
     CDS             1..142
                     /gene="LOC144111448"
                     /coded_by="XM_077644756.1:353..781"
                     /db_xref="GeneID:144111448"
ORIGIN      
        1 masgqnqtgh egsripnkvf iqrdysegts vkfqtkfpqe ldglierhmf dqtvatinsi
       61 yseaermsgk tfcegfmacl tayllymcte thyekflkqa tayiheqnet vygprglhit
      121 dpierglrvi eitilseqga rs
//
LOCUS       XP_077500904            1050 aa            linear   INV 25-NOV-2025
DEFINITION  regulator of G-protein signaling loco-like [Amblyomma americanum].
ACCESSION   XP_077500904
VERSION     XP_077500904.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644778.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1050
                     /product="regulator of G-protein signaling loco-like"
                     /calculated_mol_wt=114670
     Region          16..91
                     /region_name="PDZ_RGS12-like"
                     /note="PDZ domain of regulator of G-protein signaling 12
                     (RGS12), and related domains; cd06710"
                     /db_xref="CDD:467194"
     Site            order(26..32,40,43,72,76,79..80)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467194"
     Region          160..283
                     /region_name="PTB_RGS12"
                     /note="Regulator of G-protein signaling 12
                     Phosphotyrosine-binding (PTB) PH-like fold; cd13162"
                     /db_xref="CDD:269984"
     Site            order(165,239,258)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:269984"
     Site            order(228..233,246,276,280)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:269984"
     Region          508..621
                     /region_name="RGS"
                     /note="Regulator of G protein signaling (RGS) domain
                     superfamily; cl02565"
                     /db_xref="CDD:470619"
     Region          727..795
                     /region_name="RBD1_RGS12_like"
                     /note="Ras-binding domain (RBD) 1 of regulator of G
                     protein signaling 12 (RGS12) and similar proteins;
                     cd01817"
                     /db_xref="CDD:340515"
     Site            order(758,775)
                     /site_type="other"
                     /note="key conserved lysines"
                     /db_xref="CDD:340515"
     Region          796..871
                     /region_name="RBD2_RGS12_like"
                     /note="Ras-binding domain (RBD) 2 of regulator of G
                     protein signaling 12 (RGS12) and similar proteins;
                     cd17067"
                     /db_xref="CDD:340587"
     Site            order(807,829)
                     /site_type="other"
                     /note="key conserved lysines"
                     /db_xref="CDD:340587"
     CDS             1..1050
                     /gene="LOC144111460"
                     /coded_by="XM_077644778.1:403..3555"
                     /db_xref="GeneID:144111460"
ORIGIN      
        1 mhqtrrrkkr psygvrtvel srgkkgfgft isgqapcils civagspaer vglrpgdflv
       61 avnghnvsra phddvvrrig sssgllklqi aenyysdssd eeaatpgrth htrprhrhvp
      121 rlaardahgl fppntnqgds nkallelile qkglrtslvl rtvvgylgti elprevagsr
      181 lqairscvrr lrleqkvhtl vqlsvlrdgv lladlrgaal arfpaeriaf saayaddsrf
      241 lglvtkgadg alschvlmvd srlrlppgvl raaplhtsae pilravaaly hradgpnasp
      301 qpsqagssns snsdsgigfr degnssdrvl vvdvesqprf qsfsldrqga lpacpdaerl
      361 rarampdpvg vgsgeaplal pdwadstllg ssllddtlta adtcpdgesv dlqsstkged
      421 gsssatprrn esscssnknw trssslrrhq rrshhrrsth eagtvsdgev tseklsmsss
      481 vqsleqasds sstcdqprgr vgrwlagfde llhdplgllv faeflkkefs qeniffwvvc
      541 eqyrkmldrs erktaasaiy qkhlgsgape pvnvdsraql avqegleega pdlfvpaqkq
      601 ifnlmkfdcy qrflkselfk qcmlrevqge pvfpeaekev wklhkedkrr rsflpwhknl
      661 klvgsklger aawarrsskk kkvpkestas dlasshssla sseaslgllc sasreslpga
      721 pdsncilsrv ilpdgsstvv rvhrgetvga mlmlllqkrs ltysvvdifi agsdkpinpn
      781 vdvtlvgcke ihvepravfc vilpnskmlg vkavprrscg evlrpilaky svqlehitlr
      841 mfpvqgvtlk plsssalvad vdgqhivvqf kesgsenrsa pavrllphge svvprfldai
      901 hdgkvqfddl gvtdldilfp pvvelrqcgk shgmylppsa lrpgiekspl verlrqrets
      961 prsrddardl wvcrelvrnl daaapspppp lppkakqrgp pprppsrpqg gylsdddepn
     1021 laakplehgd fvhistkatp anhdcnisfv
//
LOCUS       XP_077500914             337 aa            linear   INV 25-NOV-2025
DEFINITION  nucleoporin NUP42-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077500914
VERSION     XP_077500914.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644788.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..337
                     /product="nucleoporin NUP42-like isoform X1"
                     /calculated_mol_wt=38158
     Region          3..23
                     /region_name="zf-CCCH_4"
                     /note="CCCH-type zinc finger; pfam18044"
                     /db_xref="CDD:465626"
     CDS             1..337
                     /gene="LOC144111471"
                     /coded_by="XM_077644788.1:100..1113"
                     /db_xref="GeneID:144111471"
ORIGIN      
        1 msvcwyymeg rcrfgdrcrf lhppleyedd eeyveprpsr sakasnfdfn aahqyarqde
       61 qyrkesyqrr ygdtyysppr nrhsnssyye yepqqqnrfq alrdtsrsds svkeaagkfd
      121 fnkafqqvak tgerrnsnev qssdeatvvs dmkqwqaggq wlfssygpfg nsvcypdfsd
      181 ismeeshldy ynacragtis dwairmkrat lnvqqkiqhl mampsdvknc lkklrdaaag
      241 avlstpsrkp vagllpapaa siaqpvvttl qpqlqaasvq skplastant qgkseanhsy
      301 tpeedltade keqflarhft pgkvplrppp sqycdld
//
LOCUS       XP_077500917             311 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111471 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077500917
VERSION     XP_077500917.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644791.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..311
                     /product="uncharacterized protein LOC144111471 isoform X2"
                     /calculated_mol_wt=34729
     CDS             1..311
                     /gene="LOC144111471"
                     /coded_by="XM_077644791.1:59..994"
                     /db_xref="GeneID:144111471"
ORIGIN      
        1 mrrmppphgl gpsrsakasn fdfnaahqya rqdeqyrkes yqrrygdtyy spprnrhsns
       61 syyeyepqqq nrfqalrdts rsdssvkeaa gkfdfnkafq qvaktgerrn snevqssdea
      121 tvvsdmkqwq aggqwlfssy gpfgnsvcyp dfsdismees hldyynacra gtisdwairm
      181 kratlnvqqk iqhlmampsd vknclkklrd aaagavlstp srkpvagllp apaasiaqpv
      241 vttlqpqlqa asvqskplas tantqgksea nhsytpeedl tadekeqfla rhftpgkvpl
      301 rpppsqycdl d
//
LOCUS       XP_077500928             456 aa            linear   INV 25-NOV-2025
DEFINITION  programmed cell death 4 [Amblyomma americanum].
ACCESSION   XP_077500928
VERSION     XP_077500928.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644802.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..456
                     /product="programmed cell death 4"
                     /calculated_mol_wt=50736
     Region          151..262
                     /region_name="MA3"
                     /note="Domain in DAP-5, eIF4G, MA-3 and other proteins;
                     smart00544"
                     /db_xref="CDD:214714"
     Region          314..427
                     /region_name="MA3"
                     /note="Domain in DAP-5, eIF4G, MA-3 and other proteins;
                     smart00544"
                     /db_xref="CDD:214714"
     CDS             1..456
                     /gene="Pdcd4"
                     /coded_by="XM_077644802.1:731..2101"
                     /db_xref="GeneID:144111486"
ORIGIN      
        1 msnggaifnn elgpreheap qlivedgesi asaemdsdik ipfddgrvkk kakrpsklia
       61 tgdvspvlpa airthpqyrk nsrrprnghg rgqpkkggag gkgtwgkpgs eisledddis
      121 aadvrdpnyd sdnqencefe sitpeltede fektvyplll eyfehgdtne vvlsleeynl
      181 rqirpnlvcl aislamerrp shremtsvll sdmygrilse pdfekgfqll lkslpdlvld
      241 tpdattvlgn flaravaddc vppkyvqlnl eetdcpltkq tlqhastlls mkhglvrldn
      301 vwgmgggmrp vkylvkkiqm llkeylcsgd vneairclrd levphfhhel vyeavvmvie
      361 dmgdmamelm ckllraldas vivtpeqmkr gfdrvfqdmp dicidvppay tvlekfigkc
      421 sgvgflspdi akamptrgrk rfvsegdggr lkeday
//
LOCUS       XP_077500942             432 aa            linear   INV 25-NOV-2025
DEFINITION  inositol polyphosphate-5-phosphatase A isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077500942
VERSION     XP_077500942.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644816.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..432
                     /product="inositol polyphosphate-5-phosphatase A isoform
                     X1"
                     /calculated_mol_wt=49260
     Region          5..392
                     /region_name="EEP"
                     /note="Exonuclease-Endonuclease-Phosphatase (EEP) domain
                     superfamily; cl00490"
                     /db_xref="CDD:469791"
     Site            order(13,50,179,227,229,345,382..383)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197306"
     Site            order(50,382)
                     /site_type="other"
                     /note="putative metal binding site [ion binding]"
                     /db_xref="CDD:197306"
     Site            order(179,229,383)
                     /site_type="other"
                     /note="putative phosphate binding site [ion binding]"
                     /db_xref="CDD:197306"
     CDS             1..432
                     /gene="5PtaseI"
                     /coded_by="XM_077644816.1:488..1786"
                     /db_xref="GeneID:144111494"
ORIGIN      
        1 mgepipvllv tanvgsifee prtllpgwig eflrtvkqyq psflalhcqe vggknyertm
       61 phvadfirtl mgseelssys avqvfldedf scvekftalg nlyfihqslk ntqiwdfqdc
      121 cfrpvtgkea hsgniekvft kekvkfpqel fpeckwsrkg fmrtrwslqg tvfdlinihl
      181 fhdesnfiam esfpssytkn rqgaldytln riqndkyenv pffifgdfnf rldtqaviek
      241 itdkappvqv ksakngdvmk vlfrdpkden kvvltverkv falqeheeaf ywndgkwlqe
      301 hdkepslfkd rlfefdiafp psypfkeess gprsymrtrc pawcdrills ksakalvytg
      361 peeggkasvv yhimgpnvcm gdhkpvvlwf rmvpaagtcp pgcvakgear kgasphrssr
      421 llrgcpagcr ll
//
LOCUS       XP_077500949             429 aa            linear   INV 25-NOV-2025
DEFINITION  inositol polyphosphate-5-phosphatase A isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077500949
VERSION     XP_077500949.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644823.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..429
                     /product="inositol polyphosphate-5-phosphatase A isoform
                     X2"
                     /calculated_mol_wt=49311
     Region          5..392
                     /region_name="EEP"
                     /note="Exonuclease-Endonuclease-Phosphatase (EEP) domain
                     superfamily; cl00490"
                     /db_xref="CDD:469791"
     Site            order(13,50,179,227,229,345,382..383)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197306"
     Site            order(50,382)
                     /site_type="other"
                     /note="putative metal binding site [ion binding]"
                     /db_xref="CDD:197306"
     Site            order(179,229,383)
                     /site_type="other"
                     /note="putative phosphate binding site [ion binding]"
                     /db_xref="CDD:197306"
     CDS             1..429
                     /gene="5PtaseI"
                     /coded_by="XM_077644823.1:488..1777"
                     /db_xref="GeneID:144111494"
ORIGIN      
        1 mgepipvllv tanvgsifee prtllpgwig eflrtvkqyq psflalhcqe vggknyertm
       61 phvadfirtl mgseelssys avqvfldedf scvekftalg nlyfihqslk ntqiwdfqdc
      121 cfrpvtgkea hsgniekvft kekvkfpqel fpeckwsrkg fmrtrwslqg tvfdlinihl
      181 fhdesnfiam esfpssytkn rqgaldytln riqndkyenv pffifgdfnf rldtqaviek
      241 itdkappvqv ksakngdvmk vlfrdpkden kvvltverkv falqeheeaf ywndgkwlqe
      301 hdkepslfkd rlfefdiafp psypfkeess gprsymrtrc pawcdrills ksakalvytg
      361 peeggkasvv yhimgpnvcm gdhkpvvlwf rmvpaaepcq kpaleeaatr yiqvkvpgsp
      421 vrifrettv
//
LOCUS       XP_077500962             199 aa            linear   INV 25-NOV-2025
DEFINITION  ras-related protein Rab-24-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077500962
VERSION     XP_077500962.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644836.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..199
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..199
                     /product="ras-related protein Rab-24-like isoform X1"
                     /calculated_mol_wt=22318
     Region          6..199
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            12..19
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:133318"
     Site            order(14..20,65,119..120,122,153..155)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:133318"
     Site            39
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:133318"
     Site            43..45
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:133318"
     Site            62..65
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:133318"
     Site            order(64..65,81..82)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:133318"
     Site            119..122
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:133318"
     Site            153..155
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:133318"
     CDS             1..199
                     /gene="LOC144111509"
                     /coded_by="XM_077644836.1:309..908"
                     /db_xref="GeneID:144111509"
ORIGIN      
        1 mshvdlkvvl lgkeyggkts lverylydrf dastpyqnti gaaygakqia vrnrritlgi
       61 wdtagserye amsriyyrga gaaivcfdlt dresyqkakf wvselrkhee kcqiflcgtk
      121 ndlikeegry raisyhdvle yadeigakmf etssktgegi aelfcqiakd yiesmatprn
      181 kpganfrlee pasqgggcc
//
LOCUS       XP_077500967             199 aa            linear   INV 25-NOV-2025
DEFINITION  ras-related protein Rab-24-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077500967
VERSION     XP_077500967.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644841.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..199
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..199
                     /product="ras-related protein Rab-24-like isoform X1"
                     /calculated_mol_wt=22318
     Region          6..199
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            12..19
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:133318"
     Site            order(14..20,65,119..120,122,153..155)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:133318"
     Site            39
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:133318"
     Site            43..45
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:133318"
     Site            62..65
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:133318"
     Site            order(64..65,81..82)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:133318"
     Site            119..122
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:133318"
     Site            153..155
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:133318"
     CDS             1..199
                     /gene="LOC144111509"
                     /coded_by="XM_077644841.1:262..861"
                     /db_xref="GeneID:144111509"
ORIGIN      
        1 mshvdlkvvl lgkeyggkts lverylydrf dastpyqnti gaaygakqia vrnrritlgi
       61 wdtagserye amsriyyrga gaaivcfdlt dresyqkakf wvselrkhee kcqiflcgtk
      121 ndlikeegry raisyhdvle yadeigakmf etssktgegi aelfcqiakd yiesmatprn
      181 kpganfrlee pasqgggcc
//
LOCUS       XP_077500975             199 aa            linear   INV 25-NOV-2025
DEFINITION  ras-related protein Rab-24-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077500975
VERSION     XP_077500975.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644849.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..199
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..199
                     /product="ras-related protein Rab-24-like isoform X1"
                     /calculated_mol_wt=22318
     Region          6..199
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            12..19
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:133318"
     Site            order(14..20,65,119..120,122,153..155)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:133318"
     Site            39
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:133318"
     Site            43..45
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:133318"
     Site            62..65
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:133318"
     Site            order(64..65,81..82)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:133318"
     Site            119..122
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:133318"
     Site            153..155
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:133318"
     CDS             1..199
                     /gene="LOC144111509"
                     /coded_by="XM_077644849.1:231..830"
                     /db_xref="GeneID:144111509"
ORIGIN      
        1 mshvdlkvvl lgkeyggkts lverylydrf dastpyqnti gaaygakqia vrnrritlgi
       61 wdtagserye amsriyyrga gaaivcfdlt dresyqkakf wvselrkhee kcqiflcgtk
      121 ndlikeegry raisyhdvle yadeigakmf etssktgegi aelfcqiakd yiesmatprn
      181 kpganfrlee pasqgggcc
//
LOCUS       XP_077500980             198 aa            linear   INV 25-NOV-2025
DEFINITION  ras-related protein Rab-24-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077500980
VERSION     XP_077500980.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644854.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..198
                     /product="ras-related protein Rab-24-like isoform X2"
                     /calculated_mol_wt=22320
     Region          6..198
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            12..19
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206648"
     Site            order(14..20,65,119..120,122,153..155)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206648"
     Site            39
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206648"
     Site            43..45
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206648"
     Site            62..65
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206648"
     Site            order(64..65,81..82)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206648"
     Site            119..122
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206648"
     Site            153..155
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206648"
     CDS             1..198
                     /gene="LOC144111509"
                     /coded_by="XM_077644854.1:234..830"
                     /db_xref="GeneID:144111509"
ORIGIN      
        1 mshvdlkvvl lgkeyggkts lverylydrf dastpyqnti gaaygakqia vrnrritlgi
       61 wdtagserye amsriyyrga gaaivcfdlt dresyqkakf wvselrkhee kcqiflcgtk
      121 ndlikeegry raisyhdvle yadeigakmf etssktgegi aelfcqiakd yiesmatprn
      181 kranfrleep asqgggcc
//
LOCUS       XP_077500981             323 aa            linear   INV 25-NOV-2025
DEFINITION  mitoferrin-2-like [Amblyomma americanum].
ACCESSION   XP_077500981
VERSION     XP_077500981.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644855.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..323
                     /product="mitoferrin-2-like"
                     /calculated_mol_wt=35428
     Region          11..103
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          107..191
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          <220..298
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     CDS             1..323
                     /gene="LOC144111551"
                     /coded_by="XM_077644855.1:225..1196"
                     /db_xref="GeneID:144111551"
ORIGIN      
        1 mefedyeslp tanvvthmla gaaagvmehc vmypldsvkt rmqslrpspg gryssipdaf
       61 ckmvrhegal rpvrgmsavv igagpahaly fscyeklkrs isgtehgtns pisqglagcl
      121 atvmhdsimn paevvkqrmq mynsqfkrct ecflhvwqhe garafyrsyt tqlsmnipfq
      181 cvhfvtyefm qvvtnkeray npmahmvsgg vagafaaavt tpldvcktll ntqetsllkt
      241 thqpqisgli naattiyscc glkgyfrglq arvlfqmpgt aiswsvyeff kaslnrrres
      301 efststsidt avasvsmgrs gti
//
LOCUS       XP_077500982             183 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111567 [Amblyomma americanum].
ACCESSION   XP_077500982
VERSION     XP_077500982.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644856.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="uncharacterized protein LOC144111567"
                     /calculated_mol_wt=21443
     CDS             1..183
                     /gene="LOC144111567"
                     /coded_by="XM_077644856.1:257..808"
                     /db_xref="GeneID:144111567"
ORIGIN      
        1 mswdahvskv clslsryigi isrnkhilpt ktklnlyyaf fysyvsycfl vwgqttasni
       61 qkllvlqkri lcvianvpyd aptrpiyqqy rmipvtrmfd yrfslfykki iaeddaffsg
      121 ivtltphrtp yvtrqrmiys lprcrtnygf stlkyilpky lntpfydtlh smsvsairnv
      181 fss
//
LOCUS       XP_077500983             831 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111599 [Amblyomma americanum].
ACCESSION   XP_077500983
VERSION     XP_077500983.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644857.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..831
                     /product="uncharacterized protein LOC144111599"
                     /calculated_mol_wt=91718
     Region          479..>774
                     /region_name="CCDC142"
                     /note="Coiled-coil protein 142; pfam14923"
                     /db_xref="CDD:464379"
     CDS             1..831
                     /gene="LOC144111599"
                     /coded_by="XM_077644857.1:260..2755"
                     /db_xref="GeneID:144111599"
ORIGIN      
        1 mgeaelppvq rrrfaserre wkkmveiqqh llltssrssk qisssssydk apqavqkqpa
       61 gpkpkappvt pvtkpppped qacssapall geqkillhta evmetlsill qsdssvcsss
      121 sgtdgrqelc sarekervka lhdqmkslmk ercllvikke cshqvatvrk lrdriknlal
      181 aedtligqll ssghpilpkd vlhlsflrks afldnlqaat fawpclvkfl ghtfnsfdaq
      241 whssldvvlr wtqvrldslt eqalrwawls lrndilllcq mdaadlhadw iggvfsschf
      301 fnqllgahgs svtpllvstv lytlaahqae tsaarlaafv ggegrkgper airaytwedv
      361 ggtdfwsmpa acdtpsttaf ltaliqsgqt lllkylyaat cidhsappqa skqkqgralw
      421 qkqppratrp scfeleklyw dhywdcfasq llarlailts specgetega paksdfslll
      481 vlacqshdlp ngavasmkcv shillaqlsl vawkqglssa lvrsyhgtvt havrdlllqa
      541 vqplsaylav asrtdckdss lakvhlaltl wyglhwaeas lwsslrswap sllphvvscd
      601 lqsiiasakh aqlswekheg paelicnvns tcksavdale clqtsvatry qdscynacqq
      661 kmksvvpaak hfqkqveskk cdyvtptldh ildpviqvlv glpadvqtly vrltleavfk
      721 awrrtlevrn lkiseqtaaq lkddaalclt rvehselnds aksglqdlst yrellelahp
      781 aqhtalasat sgrlfssnrv spllrtndaq evactpvrmr grlwhkwccn l
//
LOCUS       XP_077500984             831 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111599 [Amblyomma americanum].
ACCESSION   XP_077500984
VERSION     XP_077500984.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644858.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..831
                     /product="uncharacterized protein LOC144111599"
                     /calculated_mol_wt=91718
     Region          479..>774
                     /region_name="CCDC142"
                     /note="Coiled-coil protein 142; pfam14923"
                     /db_xref="CDD:464379"
     CDS             1..831
                     /gene="LOC144111599"
                     /coded_by="XM_077644858.1:260..2755"
                     /db_xref="GeneID:144111599"
ORIGIN      
        1 mgeaelppvq rrrfaserre wkkmveiqqh llltssrssk qisssssydk apqavqkqpa
       61 gpkpkappvt pvtkpppped qacssapall geqkillhta evmetlsill qsdssvcsss
      121 sgtdgrqelc sarekervka lhdqmkslmk ercllvikke cshqvatvrk lrdriknlal
      181 aedtligqll ssghpilpkd vlhlsflrks afldnlqaat fawpclvkfl ghtfnsfdaq
      241 whssldvvlr wtqvrldslt eqalrwawls lrndilllcq mdaadlhadw iggvfsschf
      301 fnqllgahgs svtpllvstv lytlaahqae tsaarlaafv ggegrkgper airaytwedv
      361 ggtdfwsmpa acdtpsttaf ltaliqsgqt lllkylyaat cidhsappqa skqkqgralw
      421 qkqppratrp scfeleklyw dhywdcfasq llarlailts specgetega paksdfslll
      481 vlacqshdlp ngavasmkcv shillaqlsl vawkqglssa lvrsyhgtvt havrdlllqa
      541 vqplsaylav asrtdckdss lakvhlaltl wyglhwaeas lwsslrswap sllphvvscd
      601 lqsiiasakh aqlswekheg paelicnvns tcksavdale clqtsvatry qdscynacqq
      661 kmksvvpaak hfqkqveskk cdyvtptldh ildpviqvlv glpadvqtly vrltleavfk
      721 awrrtlevrn lkiseqtaaq lkddaalclt rvehselnds aksglqdlst yrellelahp
      781 aqhtalasat sgrlfssnrv spllrtndaq evactpvrmr grlwhkwccn l
//
LOCUS       XP_077500985             590 aa            linear   INV 25-NOV-2025
DEFINITION  5' nucleotidase B isoform X1 [Amblyomma americanum].
ACCESSION   XP_077500985
VERSION     XP_077500985.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644859.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..590
                     /product="5' nucleotidase B isoform X1"
                     /calculated_mol_wt=68034
     Region          78..519
                     /region_name="5_nucleotid"
                     /note="5' nucleotidase family; pfam05761"
                     /db_xref="CDD:461733"
     CDS             1..590
                     /gene="Nt5b"
                     /coded_by="XM_077644859.1:36..1808"
                     /db_xref="GeneID:144111625"
ORIGIN      
        1 mlgcaddgds clrkdrvrea sfmeaewpaa heevhrasqt itdgmpaapf ddgtqsgapk
       61 kyyraplhri fvnrslhlek ikffgfdmdy tiaqykspdy eafqfhllvd qllsigypte
      121 lkqfqydslf pirglwfdsq ygnllkvdpy gnilvcchgf qflktgeiyk iypnkfikld
      181 esrvfvlntl fnlpetylla clvdffstsq qyiptktgvk ignlfmsyks ifqdvrgavd
      241 nihtygqlkt etinnldkyl ekderlplll drmrahdkkl flltnsdyay takvmqylfd
      301 fpstnhrtwe syfdyivvda rkplffgegt ilrqvdtstg alrigshigp lqlgqvysgg
      361 scdvftefvg akgkdvlyig dhiygdilks kktrgwrtfl mipelekein vsinrwtlfe
      421 klqeldiclg diyknldsss tdvpdigklr qsiretshel dmcygilgsm frsgsrqtff
      481 anqicryadi yaftfvnlmy ypfsymfrap pmlmphestv nheqdpthqm ngdsmldgev
      541 fkgtaesmre fakreqvphr ipetpsqith hhdtdddded aervpetqhq
//
LOCUS       XP_077500986             575 aa            linear   INV 25-NOV-2025
DEFINITION  5' nucleotidase B isoform X2 [Amblyomma americanum].
ACCESSION   XP_077500986
VERSION     XP_077500986.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644860.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..575
                     /product="5' nucleotidase B isoform X2"
                     /calculated_mol_wt=66218
     Region          63..504
                     /region_name="5_nucleotid"
                     /note="5' nucleotidase family; pfam05761"
                     /db_xref="CDD:461733"
     CDS             1..575
                     /gene="Nt5b"
                     /coded_by="XM_077644860.1:16..1743"
                     /db_xref="GeneID:144111625"
ORIGIN      
        1 mcltskpphd asaprqlgvh rasqtitdgm paapfddgtq sgapkkyyra plhrifvnrs
       61 lhlekikffg fdmdytiaqy kspdyeafqf hllvdqllsi gyptelkqfq ydslfpirgl
      121 wfdsqygnll kvdpygnilv cchgfqflkt geiykiypnk fikldesrvf vlntlfnlpe
      181 tyllaclvdf fstsqqyipt ktgvkignlf msyksifqdv rgavdnihty gqlktetinn
      241 ldkylekder lpllldrmra hdkklflltn sdyaytakvm qylfdfpstn hrtwesyfdy
      301 ivvdarkplf fgegtilrqv dtstgalrig shigplqlgq vysggscdvf tefvgakgkd
      361 vlyigdhiyg dilkskktrg wrtflmipel ekeinvsinr wtlfeklqel diclgdiykn
      421 ldssstdvpd igklrqsire tsheldmcyg ilgsmfrsgs rqtffanqic ryadiyaftf
      481 vnlmyypfsy mfrappmlmp hestvnheqd pthqmngdsm ldgevfkgta esmrefakre
      541 qvphripetp sqithhhdtd dddedaervp etqhq
//
LOCUS       XP_077500987             573 aa            linear   INV 25-NOV-2025
DEFINITION  5' nucleotidase B isoform X3 [Amblyomma americanum].
ACCESSION   XP_077500987
VERSION     XP_077500987.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644861.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..573
                     /product="5' nucleotidase B isoform X3"
                     /calculated_mol_wt=66018
     Region          61..502
                     /region_name="5_nucleotid"
                     /note="5' nucleotidase family; pfam05761"
                     /db_xref="CDD:461733"
     CDS             1..573
                     /gene="Nt5b"
                     /coded_by="XM_077644861.1:197..1918"
                     /db_xref="GeneID:144111625"
ORIGIN      
        1 masgeeicna sarenkvhra sqtitdgmpa apfddgtqsg apkkyyrapl hrifvnrslh
       61 lekikffgfd mdytiaqyks pdyeafqfhl lvdqllsigy ptelkqfqyd slfpirglwf
      121 dsqygnllkv dpygnilvcc hgfqflktge iykiypnkfi kldesrvfvl ntlfnlpety
      181 llaclvdffs tsqqyiptkt gvkignlfms yksifqdvrg avdnihtygq lktetinnld
      241 kylekderlp llldrmrahd kklflltnsd yaytakvmqy lfdfpstnhr twesyfdyiv
      301 vdarkplffg egtilrqvdt stgalrigsh igplqlgqvy sggscdvfte fvgakgkdvl
      361 yigdhiygdi lkskktrgwr tflmipelek einvsinrwt lfeklqeldi clgdiyknld
      421 ssstdvpdig klrqsirets heldmcygil gsmfrsgsrq tffanqicry adiyaftfvn
      481 lmyypfsymf rappmlmphe stvnheqdpt hqmngdsmld gevfkgtaes mrefakreqv
      541 phripetpsq ithhhdtddd dedaervpet qhq
//
LOCUS       XP_077500988             572 aa            linear   INV 25-NOV-2025
DEFINITION  5' nucleotidase B isoform X4 [Amblyomma americanum].
ACCESSION   XP_077500988
VERSION     XP_077500988.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644862.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..572
                     /product="5' nucleotidase B isoform X4"
                     /calculated_mol_wt=66099
     Region          60..501
                     /region_name="5_nucleotid"
                     /note="5' nucleotidase family; pfam05761"
                     /db_xref="CDD:461733"
     CDS             1..572
                     /gene="Nt5b"
                     /coded_by="XM_077644862.1:98..1816"
                     /db_xref="GeneID:144111625"
ORIGIN      
        1 mrttecnash tfyddvhras qtitdgmpaa pfddgtqsga pkkyyraplh rifvnrslhl
       61 ekikffgfdm dytiaqyksp dyeafqfhll vdqllsigyp telkqfqyds lfpirglwfd
      121 sqygnllkvd pygnilvcch gfqflktgei ykiypnkfik ldesrvfvln tlfnlpetyl
      181 laclvdffst sqqyiptktg vkignlfmsy ksifqdvrga vdnihtygql ktetinnldk
      241 ylekderlpl lldrmrahdk klflltnsdy aytakvmqyl fdfpstnhrt wesyfdyivv
      301 darkplffge gtilrqvdts tgalrigshi gplqlgqvys ggscdvftef vgakgkdvly
      361 igdhiygdil kskktrgwrt flmipeleke invsinrwtl feklqeldic lgdiyknlds
      421 sstdvpdigk lrqsiretsh eldmcygilg smfrsgsrqt ffanqicrya diyaftfvnl
      481 myypfsymfr appmlmphes tvnheqdpth qmngdsmldg evfkgtaesm refakreqvp
      541 hripetpsqi thhhdtdddd edaervpetq hq
//
LOCUS       XP_077500989             565 aa            linear   INV 25-NOV-2025
DEFINITION  5' nucleotidase B isoform X5 [Amblyomma americanum].
ACCESSION   XP_077500989
VERSION     XP_077500989.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644863.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..565
                     /product="5' nucleotidase B isoform X5"
                     /calculated_mol_wt=65147
     Region          53..494
                     /region_name="5_nucleotid"
                     /note="5' nucleotidase family; pfam05761"
                     /db_xref="CDD:461733"
     CDS             1..565
                     /gene="Nt5b"
                     /coded_by="XM_077644863.1:219..1916"
                     /db_xref="GeneID:144111625"
ORIGIN      
        1 masgeeicvh rasqtitdgm paapfddgtq sgapkkyyra plhrifvnrs lhlekikffg
       61 fdmdytiaqy kspdyeafqf hllvdqllsi gyptelkqfq ydslfpirgl wfdsqygnll
      121 kvdpygnilv cchgfqflkt geiykiypnk fikldesrvf vlntlfnlpe tyllaclvdf
      181 fstsqqyipt ktgvkignlf msyksifqdv rgavdnihty gqlktetinn ldkylekder
      241 lpllldrmra hdkklflltn sdyaytakvm qylfdfpstn hrtwesyfdy ivvdarkplf
      301 fgegtilrqv dtstgalrig shigplqlgq vysggscdvf tefvgakgkd vlyigdhiyg
      361 dilkskktrg wrtflmipel ekeinvsinr wtlfeklqel diclgdiykn ldssstdvpd
      421 igklrqsire tsheldmcyg ilgsmfrsgs rqtffanqic ryadiyaftf vnlmyypfsy
      481 mfrappmlmp hestvnheqd pthqmngdsm ldgevfkgta esmrefakre qvphripetp
      541 sqithhhdtd dddedaervp etqhq
//
LOCUS       XP_077500991             560 aa            linear   INV 25-NOV-2025
DEFINITION  5' nucleotidase B isoform X6 [Amblyomma americanum].
ACCESSION   XP_077500991
VERSION     XP_077500991.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644865.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..560
                     /product="5' nucleotidase B isoform X6"
                     /calculated_mol_wt=64711
     Region          48..489
                     /region_name="5_nucleotid"
                     /note="5' nucleotidase family; pfam05761"
                     /db_xref="CDD:461733"
     CDS             1..560
                     /gene="Nt5b"
                     /coded_by="XM_077644865.1:201..1883"
                     /db_xref="GeneID:144111625"
ORIGIN      
        1 mprvhrasqt itdgmpaapf ddgtqsgapk kyyraplhri fvnrslhlek ikffgfdmdy
       61 tiaqykspdy eafqfhllvd qllsigypte lkqfqydslf pirglwfdsq ygnllkvdpy
      121 gnilvcchgf qflktgeiyk iypnkfikld esrvfvlntl fnlpetylla clvdffstsq
      181 qyiptktgvk ignlfmsyks ifqdvrgavd nihtygqlkt etinnldkyl ekderlplll
      241 drmrahdkkl flltnsdyay takvmqylfd fpstnhrtwe syfdyivvda rkplffgegt
      301 ilrqvdtstg alrigshigp lqlgqvysgg scdvftefvg akgkdvlyig dhiygdilks
      361 kktrgwrtfl mipelekein vsinrwtlfe klqeldiclg diyknldsss tdvpdigklr
      421 qsiretshel dmcygilgsm frsgsrqtff anqicryadi yaftfvnlmy ypfsymfrap
      481 pmlmphestv nheqdpthqm ngdsmldgev fkgtaesmre fakreqvphr ipetpsqith
      541 hhdtdddded aervpetqhq
//
LOCUS       XP_077500992             546 aa            linear   INV 25-NOV-2025
DEFINITION  5' nucleotidase B isoform X7 [Amblyomma americanum].
ACCESSION   XP_077500992
VERSION     XP_077500992.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644866.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..546
                     /product="5' nucleotidase B isoform X7"
                     /calculated_mol_wt=63160
     Region          34..475
                     /region_name="5_nucleotid"
                     /note="5' nucleotidase family; pfam05761"
                     /db_xref="CDD:461733"
     CDS             1..546
                     /gene="Nt5b"
                     /coded_by="XM_077644866.1:130..1770"
                     /db_xref="GeneID:144111625"
ORIGIN      
        1 mpaapfddgt qsgapkkyyr aplhrifvnr slhlekikff gfdmdytiaq ykspdyeafq
       61 fhllvdqlls igyptelkqf qydslfpirg lwfdsqygnl lkvdpygnil vcchgfqflk
      121 tgeiykiypn kfikldesrv fvlntlfnlp etyllaclvd ffstsqqyip tktgvkignl
      181 fmsyksifqd vrgavdniht ygqlktetin nldkylekde rlpllldrmr ahdkklfllt
      241 nsdyaytakv mqylfdfpst nhrtwesyfd yivvdarkpl ffgegtilrq vdtstgalri
      301 gshigplqlg qvysggscdv ftefvgakgk dvlyigdhiy gdilkskktr gwrtflmipe
      361 lekeinvsin rwtlfeklqe ldiclgdiyk nldssstdvp digklrqsir etsheldmcy
      421 gilgsmfrsg srqtffanqi cryadiyaft fvnlmyypfs ymfrappmlm phestvnheq
      481 dpthqmngds mldgevfkgt aesmrefakr eqvphripet psqithhhdt ddddedaerv
      541 petqhq
//
LOCUS       XP_077500993             339 aa            linear   INV 25-NOV-2025
DEFINITION  TBC1 domain family member 12-like [Amblyomma americanum].
ACCESSION   XP_077500993
VERSION     XP_077500993.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644867.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..339
                     /product="TBC1 domain family member 12-like"
                     /calculated_mol_wt=39429
     Region          58..284
                     /region_name="TBC"
                     /note="Domain in Tre-2, BUB2p, and Cdc16p. Probable
                     Rab-GAPs; smart00164"
                     /db_xref="CDD:214540"
     CDS             1..339
                     /gene="LOC144111702"
                     /coded_by="XM_077644867.1:685..1704"
                     /db_xref="GeneID:144111702"
ORIGIN      
        1 mvkaarkkel kdararkkqm qqqwkqeeql aqatrtwisd vlpcwevarn qrrtrdlwwq
       61 glppsirgrv wklaigneln itpelyeica srsrkiwhse avpeegsalh trehsahlir
      121 ldvsrtfpql gifqeagpyf dvlhcilgay vvyrpdigyv qgmsflaaml llnldvadaf
      181 icfanllnrp cqlaffrvdq qqmnayytly deffrenlpk lfahfkkhnl tsdlylvdwi
      241 ytlysrslpl dvacrvwdvf lrdgeeflfr salgilrlye dvllrldfin laqfltklpe
      301 dmsadvlfda isairmtvnk rnfaqvlakh resagtcqs
//
LOCUS       XP_077500994             339 aa            linear   INV 25-NOV-2025
DEFINITION  TBC1 domain family member 12-like [Amblyomma americanum].
ACCESSION   XP_077500994
VERSION     XP_077500994.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644868.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..339
                     /product="TBC1 domain family member 12-like"
                     /calculated_mol_wt=39429
     Region          58..284
                     /region_name="TBC"
                     /note="Domain in Tre-2, BUB2p, and Cdc16p. Probable
                     Rab-GAPs; smart00164"
                     /db_xref="CDD:214540"
     CDS             1..339
                     /gene="LOC144111702"
                     /coded_by="XM_077644868.1:685..1704"
                     /db_xref="GeneID:144111702"
ORIGIN      
        1 mvkaarkkel kdararkkqm qqqwkqeeql aqatrtwisd vlpcwevarn qrrtrdlwwq
       61 glppsirgrv wklaigneln itpelyeica srsrkiwhse avpeegsalh trehsahlir
      121 ldvsrtfpql gifqeagpyf dvlhcilgay vvyrpdigyv qgmsflaaml llnldvadaf
      181 icfanllnrp cqlaffrvdq qqmnayytly deffrenlpk lfahfkkhnl tsdlylvdwi
      241 ytlysrslpl dvacrvwdvf lrdgeeflfr salgilrlye dvllrldfin laqfltklpe
      301 dmsadvlfda isairmtvnk rnfaqvlakh resagtcqs
//
LOCUS       XP_077500995             635 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111728 [Amblyomma americanum].
ACCESSION   XP_077500995
VERSION     XP_077500995.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644869.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..635
                     /product="uncharacterized protein LOC144111728"
                     /calculated_mol_wt=69242
     Region          72..>308
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          488..635
                     /region_name="SET_KMT2A_2B"
                     /note="SET domain (including post-SET domain) found in
                     histone-lysine N-methyltransferase 2A (KMT2A), 2B (KMT2B)
                     and similar proteins; cd19170"
                     /db_xref="CDD:380947"
     Site            order(506..509,526,551..556,570..575,583..585,594,
                     610..615,622..625,634)
                     /site_type="active"
                     /db_xref="CDD:380947"
     Site            order(506..509,551,571..575,612,622..625)
                     /site_type="other"
                     /note="SAM binding site"
                     /db_xref="CDD:380947"
     Site            order(526,551..556,570..571,573,583..585,594,610..615)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:380947"
     Site            order(577,623,625,630)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:380947"
     CDS             1..635
                     /gene="LOC144111728"
                     /coded_by="XM_077644869.1:486..2393"
                     /db_xref="GeneID:144111728"
ORIGIN      
        1 mmacpkvila kstaavgglp aggplgqpaa malhasafcp innvglnrvi applsnytms
       61 yvqptlfsst psypskydvf qvpggtlvvp rsadltsyla pppppqqlfy splslqpqps
      121 hyaflsntav qapplvpfsl qppeqptfps fvnnafvsta syasppeplp ssygsapsnd
      181 lfltggthll qptdpfpkvc plleskqetp eqtsyaaaap fanfhgdtst spapfstsgp
      241 ysspkslpfp ktglpaprpi lacpraqeaa npvpslpapc prplqrpfep dsrfsmgtlr
      301 tepepplchg eetrlcfgsp plsasslssl dnassadtvr vtltdssstl efppvelkvp
      361 depiapvlpt tppvptpvpt pppstpqlle nvdkvhdiya fetnsdeesg vpkfkfqkrh
      421 evakycyagc artqpfrrrg sydmfdflas vhrkapapef hckdeetdec vqrrrsiqdk
      481 elatiisfhr lkdsakatvg vyrslihgrg lysmrdidag emvieyagev irsvltdkre
      541 rlyesrgigc ymfridedqv vdatthgnaa rfinhscdpn cfskvitvyg qkhiiiyalr
      601 kihkgeelty dykfpkedvk ipctcgarrc rkfln
//
LOCUS       XP_077500996             402 aa            linear   INV 25-NOV-2025
DEFINITION  F-box/WD repeat-containing protein 4 [Amblyomma americanum].
ACCESSION   XP_077500996
VERSION     XP_077500996.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644870.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..402
                     /product="F-box/WD repeat-containing protein 4"
                     /calculated_mol_wt=45281
     Region          7..53
                     /region_name="F-box_SF"
                     /note="F-box domain superfamily; cl45894"
                     /db_xref="CDD:459239"
     Site            order(11..12,14..16,19..20,23,34..36,38,41)
                     /site_type="other"
                     /note="F-box motif"
                     /db_xref="CDD:438789"
     Site            order(11,15,18..19,22..23,27,29..30,34..36,38)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438789"
     Region          136..378
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          189..225
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          229..269
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          277..314
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          321..357
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..402
                     /gene="LOC144111744"
                     /coded_by="XM_077644870.1:218..1426"
                     /db_xref="GeneID:144111744"
ORIGIN      
        1 mscsdpmfvl lppdvlyvvc sycdvrtlgr lscvckrfna flkhsyvwsr rsrnivatnq
       61 tdrrmlqrsr hllepvekcw qslrwqtgry hervllqhyt iympwlqler dalwyskgaa
      121 ilkfkrladg slgenvlltl rghaddvgrf vcknglvvsg gndgslciwt atqglsvhnr
      181 whcnskains vdysnnivvt gsqdrtvkvw tlnggssslr ytipiwdrvc svalhesnri
      241 lltgsagcng ieplqafdls sgsrvailgt nhrngagvlh vypesptevl scgydtfirl
      301 wdmrcptrcv sswedphdsa vyclatdhnv tvlsgtsryg vvrlwdkrmt spvkmyyvgr
      361 gnspvyslaf dpcymyvald rslnmlsftg sswtpqatne vf
//
LOCUS       XP_077500997             354 aa            linear   INV 25-NOV-2025
DEFINITION  DENN domain-containing protein 10-like [Amblyomma americanum].
ACCESSION   XP_077500997
VERSION     XP_077500997.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644871.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..354
                     /product="DENN domain-containing protein 10-like"
                     /calculated_mol_wt=40510
     Region          156..262
                     /region_name="SPA"
                     /note="Stabilization of polarity axis; cl40631"
                     /db_xref="CDD:454797"
     CDS             1..354
                     /gene="LOC144111760"
                     /coded_by="XM_077644871.1:140..1204"
                     /db_xref="GeneID:144111760"
ORIGIN      
        1 maavddlkav gviekdtnnd vlwtwsypsf tsqyrsllla kcdlendsms serpvwfryc
       61 hhhrdwfymy qtevfdsdnl srvklfvlvl htkdfspeky etlsrilskv ycktgdptal
      121 lrlflsvsvk gsctteengt fllsnfdrrr smthvpikdi ikmfgletii iytalilkkr
      181 vivyhhklnv llnyvralta lsqhkpeweh fypfmelsai evaelsemth ylagfrdasi
      241 egrlelydvf vnlaaieitv aphskdafqm skmhkeiamf mvrqadndnl sdqdvvqdia
      301 akteellhnm ksamapgtsg kpvvsfaklq elklapalen fywnlavaeg lvda
//
LOCUS       XP_077500998             329 aa            linear   INV 25-NOV-2025
DEFINITION  succinyl-coenzyme A synthetase alpha subunit 1 isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077500998
VERSION     XP_077500998.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644872.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..329
                     /product="succinyl-coenzyme A synthetase alpha subunit 1
                     isoform X1"
                     /calculated_mol_wt=34256
     Region          10..327
                     /region_name="PTZ00187"
                     /note="succinyl-CoA synthetase alpha subunit; Provisional"
                     /db_xref="CDD:240307"
     CDS             1..329
                     /gene="Scsalpha1"
                     /coded_by="XM_077644872.1:54..1043"
                     /db_xref="GeneID:144111776"
ORIGIN      
        1 maccrfvsra sasmllsfpl gvrtcytntr nalkinkdtk vicqgftgkq gtfhskqale
       61 ygtrlvagvs pgkggqthlg lpvfnsvkea raqtgadasv iyvpapgaak aileameaei
      121 plvvcitegi pqqdmvrvkn qlvkqdktrl vgpncpgiik peeckigimp ghihqkgkig
      181 ivsrsgtlty eavhqttqvg lgqslcvgig gdpfngtnfi dcidiflndp dtkgiilige
      241 iggnaeesaa eflekhnsga nakpvvsfia gltappgrrm ghagaiiagg kggakekiea
      301 lkaagvtvtk spaqmgaaml qemqkrgla
//
LOCUS       XP_077500999             325 aa            linear   INV 25-NOV-2025
DEFINITION  succinyl-coenzyme A synthetase alpha subunit 1 isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077500999
VERSION     XP_077500999.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644873.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..325
                     /product="succinyl-coenzyme A synthetase alpha subunit 1
                     isoform X2"
                     /calculated_mol_wt=33825
     Region          18..323
                     /region_name="PTZ00187"
                     /note="succinyl-CoA synthetase alpha subunit; Provisional"
                     /db_xref="CDD:240307"
     CDS             1..325
                     /gene="Scsalpha1"
                     /coded_by="XM_077644873.1:96..1073"
                     /db_xref="GeneID:144111776"
ORIGIN      
        1 maccrfvsra satfplgvrt cytntrnalk inkdtkvicq gftgkqgtfh skqaleygtr
       61 lvagvspgkg gqthlglpvf nsvkearaqt gadasviyvp apgaakaile ameaeiplvv
      121 citegipqqd mvrvknqlvk qdktrlvgpn cpgiikpeec kigimpghih qkgkigivsr
      181 sgtltyeavh qttqvglgqs lcvgiggdpf ngtnfidcid iflndpdtkg iiligeiggn
      241 aeesaaefle khnsganakp vvsfiaglta ppgrrmghag aiiaggkgga kekiealkaa
      301 gvtvtkspaq mgaamlqemq krgla
//
LOCUS       XP_077501000             485 aa            linear   INV 25-NOV-2025
DEFINITION  repressed by TOR [Amblyomma americanum].
ACCESSION   XP_077501000
VERSION     XP_077501000.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644874.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..485
                     /product="repressed by TOR"
                     /calculated_mol_wt=52871
     Region          378..>416
                     /region_name="bZIP_AUREO-like"
                     /note="Basic leucine zipper (bZIP) domain of blue light
                     (BL) receptor aureochrome (AUREO) and similar bZIP
                     domains; cd14809"
                     /db_xref="CDD:269871"
     Site            order(381,383..385,387..392,394..396)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269871"
     CDS             1..485
                     /gene="REPTOR"
                     /coded_by="XM_077644874.1:301..1758"
                     /db_xref="GeneID:144111819"
ORIGIN      
        1 mlgsadwaaa rgwapaaara aqdplrlpel egnvdpdqwl svegfsfepa lyapepkeek
       61 adlaeafwel eaddpcsrvp dknelgdqsp sfwgmdfels kldamlkpdk qpplkdptla
      121 elnadesgld gldvndilrd fqqqaswsnf aapsqladll arpakveplt tpalssslpa
      181 stclataepq raalaspepc rpspaaqsvp sdvpasgkpr srilaerlsc sapesqlsql
      241 smdegfssqd sdealtsdae shasqdatep gspdgqkkkk ryfwqynvqa kgpkgprlsm
      301 ardsadphvl hdvtdpvfsp echlegvkha gkarrgdgnd ltpnprklyn igmelkklgr
      361 vineltpvse lpfnarhksr keknklasra crlkkkaqhe anklklyglq qehrklitlf
      421 eeakkllreq vechqgsrre assvhfdalv krhvsvkvag hstefvnrvl ssvaagnpdg
      481 glsel
//
LOCUS       XP_077501002             243 aa            linear   INV 25-NOV-2025
DEFINITION  vesicle transport protein Sec20 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501002
VERSION     XP_077501002.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644876.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..243
                     /product="vesicle transport protein Sec20 isoform X1"
                     /calculated_mol_wt=27434
     Region          142..>203
                     /region_name="SNARE"
                     /note="SNARE motif; cl22856"
                     /db_xref="CDD:473982"
     Site            order(173,180,183)
                     /site_type="other"
                     /note="flanking leucine-zipper layers"
                     /db_xref="CDD:277192"
     Site            176
                     /site_type="active"
                     /note="zero layer [active]"
                     /db_xref="CDD:277192"
     CDS             1..243
                     /gene="Sec20"
                     /coded_by="XM_077644876.1:120..851"
                     /db_xref="GeneID:144111835"
ORIGIN      
        1 maslwrsnir nasnsantet sflqellkld lrvknciqdi qndcdsreqf nainleaaes
       61 mqkfkktlea lksfakeqdk tedrerllrk vddcvlgmkl ninalrkasl aveksiddqy
      121 rerllsgghv kqrgradket llrstsgmte nlftisrlma dqvkhsenal dllvntstvi
      181 tesqeesrqm ssailqskkl lsnedsfdll gqphsfykll qrkivawpvq cfnisspvpt
      241 pgv
//
LOCUS       XP_077501003             235 aa            linear   INV 25-NOV-2025
DEFINITION  vesicle transport protein Sec20 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077501003
VERSION     XP_077501003.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644877.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..235
                     /product="vesicle transport protein Sec20 isoform X2"
                     /calculated_mol_wt=26870
     Region          142..234
                     /region_name="SNARE_SEC20"
                     /note="SNARE motif of SEC20; cd15865"
                     /db_xref="CDD:277218"
     Site            order(147..149,151..156,158..159,162..163,165..170,
                     172..173,175..177,179..184,186..187,189..191,193..194,
                     196..198,200..201,203)
                     /site_type="other"
                     /note="heterotetramer interface [polypeptide binding]"
                     /db_xref="CDD:277218"
     Site            176
                     /site_type="other"
                     /note="zero layer"
                     /db_xref="CDD:277218"
     CDS             1..235
                     /gene="Sec20"
                     /coded_by="XM_077644877.1:120..827"
                     /db_xref="GeneID:144111835"
ORIGIN      
        1 maslwrsnir nasnsantet sflqellkld lrvknciqdi qndcdsreqf nainleaaes
       61 mqkfkktlea lksfakeqdk tedrerllrk vddcvlgmkl ninalrkasl aveksiddqy
      121 rerllsgghv kqrgradket llrstsgmte nlftisrlma dqvkhsenal dllvntstvi
      181 tesqeesrqm ssailqskkl lskygrremt drvliilall fffacvlyvl krrlf
//
LOCUS       XP_077501004             206 aa            linear   INV 25-NOV-2025
DEFINITION  vesicle transport protein Sec20 isoform X3 [Amblyomma americanum].
ACCESSION   XP_077501004
VERSION     XP_077501004.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644878.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..206
                     /product="vesicle transport protein Sec20 isoform X3"
                     /calculated_mol_wt=23240
     Region          142..>203
                     /region_name="SNARE"
                     /note="SNARE motif; cl22856"
                     /db_xref="CDD:473982"
     Site            order(173,180,183)
                     /site_type="other"
                     /note="flanking leucine-zipper layers"
                     /db_xref="CDD:277192"
     Site            176
                     /site_type="active"
                     /note="zero layer [active]"
                     /db_xref="CDD:277192"
     CDS             1..206
                     /gene="Sec20"
                     /coded_by="XM_077644878.1:120..740"
                     /db_xref="GeneID:144111835"
ORIGIN      
        1 maslwrsnir nasnsantet sflqellkld lrvknciqdi qndcdsreqf nainleaaes
       61 mqkfkktlea lksfakeqdk tedrerllrk vddcvlgmkl ninalrkasl aveksiddqy
      121 rerllsgghv kqrgradket llrstsgmte nlftisrlma dqvkhsenal dllvntstvi
      181 tesqeesrqm ssailqskkl lsktts
//
LOCUS       XP_077501010             206 aa            linear   INV 25-NOV-2025
DEFINITION  vesicle transport protein Sec20 isoform X4 [Amblyomma americanum].
ACCESSION   XP_077501010
VERSION     XP_077501010.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644884.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..206
                     /product="vesicle transport protein Sec20 isoform X4"
                     /calculated_mol_wt=23323
     Region          142..>173
                     /region_name="SNARE"
                     /note="SNARE motif; cl22856"
                     /db_xref="CDD:473982"
     CDS             1..206
                     /gene="Sec20"
                     /coded_by="XM_077644884.1:120..740"
                     /db_xref="GeneID:144111835"
ORIGIN      
        1 maslwrsnir nasnsantet sflqellkld lrvknciqdi qndcdsreqf nainleaaes
       61 mqkfkktlea lksfakeqdk tedrerllrk vddcvlgmkl ninalrkasl aveksiddqy
      121 rerllsgghv kqrgradket llrstsgmte nlftisrlma dqvkhsenal dllgqphsfy
      181 kllqrkivaw pvqcfnissp vptpgv
//
LOCUS       XP_077501021             188 aa            linear   INV 25-NOV-2025
DEFINITION  vesicle transport protein Sec20 isoform X5 [Amblyomma americanum].
ACCESSION   XP_077501021
VERSION     XP_077501021.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644895.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..188
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..188
                     /product="vesicle transport protein Sec20 isoform X5"
                     /calculated_mol_wt=21410
     Region          142..>175
                     /region_name="SNARE"
                     /note="SNARE motif; cl22856"
                     /db_xref="CDD:473982"
     CDS             1..188
                     /gene="Sec20"
                     /coded_by="XM_077644895.1:120..686"
                     /db_xref="GeneID:144111835"
ORIGIN      
        1 maslwrsnir nasnsantet sflqellkld lrvknciqdi qndcdsreqf nainleaaes
       61 mqkfkktlea lksfakeqdk tedrerllrk vddcvlgmkl ninalrkasl aveksiddqy
      121 rerllsgghv kqrgradket llrstsgmte nlftisrlma dqvkhsenal dllvsrmhvt
      181 kwhgcrkg
//
LOCUS       XP_077501025            1092 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111871 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501025
VERSION     XP_077501025.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644899.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1092
                     /product="uncharacterized protein LOC144111871 isoform X1"
                     /calculated_mol_wt=122745
     Region          18..533
                     /region_name="PLN02568"
                     /note="polyamine oxidase"
                     /db_xref="CDD:215308"
     Region          573..1070
                     /region_name="PLN02568"
                     /note="polyamine oxidase"
                     /db_xref="CDD:215308"
     CDS             1..1092
                     /gene="LOC144111871"
                     /coded_by="XM_077644899.1:245..3523"
                     /db_xref="GeneID:144111871"
ORIGIN      
        1 masseelfev ggryeisakv liigagvagl taanrlfahg frnvtilear drpggrtfsk
       61 pfgdsyiecg aqwihgqdgn avfhlaneng lvdhdcpyyl ehfytwaqlt keqghvatev
      121 ssivlealdr cqkmstsgrr dsmpgvfqks vghflrhsll eyisknsfse deiklveacy
      181 dwavrlqqei qgcssltdvs alffgqyhec dgnivtelkh gyqkfvelil qgipsewlql
      241 gkcvtnvtts vapltsnncn iteksqshlk tsfaavplgs nkpvitvtce dgsvfkadhv
      301 fvtlplgclk ahasrmfepp lpekkmlair slgfgtvnkv flkydvpfwn pgdvfqvlwl
      361 dgfnhcgnkv edddmsvwvt hsqlntswfr yigrfnavrh qpnllcawit gegakymetl
      421 sdddvrtgch nvlvhvlgnk dlpvpsever skwfsdpyar gsysyisvsc dttgslprdl
      481 aepicepvvh cgtevtypvi ffageathph fystvhgaye sglreadrla dfyfkagsgq
      541 esccvpdeee sllvkadpfs saalnassag snpriiivga gaaglsaayk ltqkgytnfv
      601 ileaqkmagg riqsyyyddn kvlelgaqwv hgeegnplyg yalsndllad prryhslegr
      661 gffctdqgtr lpqdlvdevi tvlnqikeel ggrrprlegn qevfevndlp isvgeylrsr
      721 flehleqqsn tadmvkikwa iydwywrfev idnscyslde lsfksyeefe ecpgvwninl
      781 rhgfssvins llehipeanv rynkavkriy wdnsaipaha kmprssisns qetiwesipf
      841 vecedgeiis crhllltmsa gylkrhlddm fhpelpkekc qalrsigfgt inkiflifeq
      901 pfwdpgtegf qliwlddese dttkpdwwvr gisgfdlvye npnvlvgwig gkaaehmell
      961 ddsevldacv svlskflhre ipapvsivrs ywftnpyivg sysnkqlpyd asdalletfy
     1021 eplvadaplh rvtkvewplv lfageatdkd fystvhgamr sgfreadrli nfwsrkrddi
     1081 ilessqaqpp rl
//
LOCUS       XP_077501031            1091 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111871 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501031
VERSION     XP_077501031.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644905.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1091
                     /product="uncharacterized protein LOC144111871 isoform X2"
                     /calculated_mol_wt=122658
     Region          18..533
                     /region_name="PLN02568"
                     /note="polyamine oxidase"
                     /db_xref="CDD:215308"
     Region          573..1074
                     /region_name="PLN02568"
                     /note="polyamine oxidase"
                     /db_xref="CDD:215308"
     CDS             1..1091
                     /gene="LOC144111871"
                     /coded_by="XM_077644905.1:96..3371"
                     /db_xref="GeneID:144111871"
ORIGIN      
        1 masseelfev ggryeisakv liigagvagl taanrlfahg frnvtilear drpggrtfsk
       61 pfgdsyiecg aqwihgqdgn avfhlaneng lvdhdcpyyl ehfytwaqlt keqghvatev
      121 ssivlealdr cqkmstsgrr dsmpgvfqks vghflrhsll eyisknsfse deiklveacy
      181 dwavrlqqei qgcssltdvs alffgqyhec dgnivtelkh gyqkfvelil qgipsewlql
      241 gkcvtnvtts vapltsnncn iteksqshlk tsfaavplgs nkpvitvtce dgsvfkadhv
      301 fvtlplgclk ahasrmfepp lpekkmlair slgfgtvnkv flkydvpfwn pgdvfqvlwl
      361 dgfnhcgnkv edddmsvwvt hsqlntswfr yigrfnavrh qpnllcawit gegakymetl
      421 sdddvrtgch nvlvhvlgnk dlpvpsever skwfsdpyar gsysyisvsc dttgslprdl
      481 aepicepvvh cgtevtypvi ffageathph fystvhgaye sglreadrla dfyfkagsgq
      541 esccvpdeee sllvkadpfs saalnassag snpriiivga gaaglsaayk ltqkgytnfv
      601 ileaqkmagg riqsyyyddn kvlelgaqwv hgeegnplyg yalsndllad prryhslegr
      661 gffctdqgtr lpqdlvdevi tvlnqikeel ggrrprlegn qevfevndlp isvgeylrsr
      721 flehleqqsn tadmvkikwa iydwywrfev idnscyslde lsfksyeefe ecpgvwninl
      781 rhgfssvins llehipeanv rynkavkriy wdnsaipaha kmprssisns qetiwesipf
      841 vecedgeiis crhllltmsa gylkrhlddm fhpelpkekc qalrsigfgt inkiflifeq
      901 pfwdpgtegf qliwlddese dttkpdwwvr gisgfdlvye npnvlvgwig gkaaehmell
      961 ddsevldacv svlskflhre ipapvsivrs ywftnpyivg sysnkqlpyd asdalletfy
     1021 eplvadaplh rvtkvewplv lfageatdkd fystvhgamr sgfreadrli nfwrkrddii
     1081 lessqaqppr l
//
LOCUS       XP_077501041             777 aa            linear   INV 25-NOV-2025
DEFINITION  aminopeptidase P isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501041
VERSION     XP_077501041.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644915.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..777
                     /product="aminopeptidase P isoform X1"
                     /calculated_mol_wt=87781
     Region          5..63
                     /region_name="FLYWCH"
                     /note="FLYWCH zinc finger domain; pfam04500"
                     /db_xref="CDD:461332"
     Region          171..298
                     /region_name="Creatinase_N"
                     /note="Creatinase/Prolidase N-terminal domain; pfam01321"
                     /db_xref="CDD:460159"
     Region          318..482
                     /region_name="Creatinase_N_2"
                     /note="Creatinase/Prolidase N-terminal domain; pfam16189"
                     /db_xref="CDD:465053"
     Region          486..709
                     /region_name="APP"
                     /note="X-Prolyl Aminopeptidase 2. E.C. 3.4.11.9. Also
                     known as X-Pro aminopeptidase, proline aminopeptidase,
                     aminopeptidase P, and aminoacylproline aminopeptidase.
                     Catalyses release of any N-terminal amino acid, including
                     proline, that is linked with proline; cd01085"
                     /db_xref="CDD:238518"
     Site            order(551,571,582,649,680,694)
                     /site_type="active"
                     /db_xref="CDD:238518"
     Region          712..776
                     /region_name="Peptidase_M24_C"
                     /note="C-terminal region of peptidase_M24; pfam16188"
                     /db_xref="CDD:465052"
     CDS             1..777
                     /gene="ApepP"
                     /coded_by="XM_077644915.1:136..2469"
                     /db_xref="GeneID:144111884"
ORIGIN      
        1 mecqyvatqr ggiallyegh rynkvrdgke gtvywrcard rqcpgravtv ysrikkannk
       61 hnhppdawrn kvdqvvsdlk lraqddattl ptlftetlky lasshqvaal amptfpsfkg
      121 tvckprhqlv psllqlrdfr pednwlyant adepstsepl dmalrntgtl lkrlralmkn
      181 ttyvsetiqa yivpsgdahq seyiapcdkr rafltgftgs agtaivtedh aalwtdgryf
      241 lqaeqqldsn wvlmkdgipg tpsqgewlck vlssgsrvgv dpflmpydaw kqlsnqldvs
      301 ghclvpvaqn lvdliweerp sppsrpldsl siiytgkfwq ekvadirqdm sqksasvlvl
      361 taldeiawlf nlrgsdidyn pvffayavvt mdsaylfide nklsatlqrh lstdrneknl
      421 tvdirpyrvf kdflsllinq qsgkfwvsss ssyavvsqvp kerriesttp vmlrkaikne
      481 teiecmrrah ikdavalcef fvwmesevpk gevtemtaaa klehfrreqe dyvgpsfeti
      541 sasgpnaaii hyrpeeesdr rvtteeiylc dsggqyrdgt tdvtrtwhfg tpsqyekecf
      601 trvvkgnial ssaifprlvk gqmldtlarr alwevgldyl hgtghgvgaf lnvhegpmgi
      661 swmphpndpg lqegmilsie pgyyedgqfg irienlalvr kaatkynfkd rgflafdslt
      721 lvpiqtkmln plmltaeeve wldtyhqacr dvigraleeq grdvalqwll retqplg
//
LOCUS       XP_077501048             776 aa            linear   INV 25-NOV-2025
DEFINITION  aminopeptidase P isoform X2 [Amblyomma americanum].
ACCESSION   XP_077501048
VERSION     XP_077501048.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644922.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..776
                     /product="aminopeptidase P isoform X2"
                     /calculated_mol_wt=87710
     Region          5..63
                     /region_name="FLYWCH"
                     /note="FLYWCH zinc finger domain; pfam04500"
                     /db_xref="CDD:461332"
     Region          170..297
                     /region_name="Creatinase_N"
                     /note="Creatinase/Prolidase N-terminal domain; pfam01321"
                     /db_xref="CDD:460159"
     Region          317..481
                     /region_name="Creatinase_N_2"
                     /note="Creatinase/Prolidase N-terminal domain; pfam16189"
                     /db_xref="CDD:465053"
     Region          485..708
                     /region_name="APP"
                     /note="X-Prolyl Aminopeptidase 2. E.C. 3.4.11.9. Also
                     known as X-Pro aminopeptidase, proline aminopeptidase,
                     aminopeptidase P, and aminoacylproline aminopeptidase.
                     Catalyses release of any N-terminal amino acid, including
                     proline, that is linked with proline; cd01085"
                     /db_xref="CDD:238518"
     Site            order(550,570,581,648,679,693)
                     /site_type="active"
                     /db_xref="CDD:238518"
     Region          711..775
                     /region_name="Peptidase_M24_C"
                     /note="C-terminal region of peptidase_M24; pfam16188"
                     /db_xref="CDD:465052"
     CDS             1..776
                     /gene="ApepP"
                     /coded_by="XM_077644922.1:136..2466"
                     /db_xref="GeneID:144111884"
ORIGIN      
        1 mecqyvatqr ggiallyegh rynkvrdgke gtvywrcard rqcpgravtv ysrikkannk
       61 hnhppdawrn kvdqvvsdlk lraqddattl ptlftetlky lasshqvaal amptfpsfkg
      121 tvckprhqlv psllqlrdfr pednwlyant depstsepld malrntgtll krlralmknt
      181 tyvsetiqay ivpsgdahqs eyiapcdkrr afltgftgsa gtaivtedha alwtdgryfl
      241 qaeqqldsnw vlmkdgipgt psqgewlckv lssgsrvgvd pflmpydawk qlsnqldvsg
      301 hclvpvaqnl vdliweerps ppsrpldsls iiytgkfwqe kvadirqdms qksasvlvlt
      361 aldeiawlfn lrgsdidynp vffayavvtm dsaylfiden klsatlqrhl stdrneknlt
      421 vdirpyrvfk dflsllinqq sgkfwvssss syavvsqvpk erriesttpv mlrkaiknet
      481 eiecmrrahi kdavalceff vwmesevpkg evtemtaaak lehfrreqed yvgpsfetis
      541 asgpnaaiih yrpeeesdrr vtteeiylcd sggqyrdgtt dvtrtwhfgt psqyekecft
      601 rvvkgnials saifprlvkg qmldtlarra lwevgldylh gtghgvgafl nvhegpmgis
      661 wmphpndpgl qegmilsiep gyyedgqfgi rienlalvrk aatkynfkdr gflafdsltl
      721 vpiqtkmlnp lmltaeevew ldtyhqacrd vigraleeqg rdvalqwllr etqplg
//
LOCUS       XP_077501057             616 aa            linear   INV 25-NOV-2025
DEFINITION  aminopeptidase P isoform X3 [Amblyomma americanum].
ACCESSION   XP_077501057
VERSION     XP_077501057.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644931.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..616
                     /product="aminopeptidase P isoform X3"
                     /calculated_mol_wt=69438
     Region          10..137
                     /region_name="Creatinase_N"
                     /note="Creatinase/Prolidase N-terminal domain; pfam01321"
                     /db_xref="CDD:460159"
     Region          157..321
                     /region_name="Creatinase_N_2"
                     /note="Creatinase/Prolidase N-terminal domain; pfam16189"
                     /db_xref="CDD:465053"
     Region          325..548
                     /region_name="APP"
                     /note="X-Prolyl Aminopeptidase 2. E.C. 3.4.11.9. Also
                     known as X-Pro aminopeptidase, proline aminopeptidase,
                     aminopeptidase P, and aminoacylproline aminopeptidase.
                     Catalyses release of any N-terminal amino acid, including
                     proline, that is linked with proline; cd01085"
                     /db_xref="CDD:238518"
     Site            order(390,410,421,488,519,533)
                     /site_type="active"
                     /db_xref="CDD:238518"
     Region          551..615
                     /region_name="Peptidase_M24_C"
                     /note="C-terminal region of peptidase_M24; pfam16188"
                     /db_xref="CDD:465052"
     CDS             1..616
                     /gene="ApepP"
                     /coded_by="XM_077644931.1:258..2108"
                     /db_xref="GeneID:144111884"
ORIGIN      
        1 malrntgtll krlralmknt tyvsetiqay ivpsgdahqs eyiapcdkrr afltgftgsa
       61 gtaivtedha alwtdgryfl qaeqqldsnw vlmkdgipgt psqgewlckv lssgsrvgvd
      121 pflmpydawk qlsnqldvsg hclvpvaqnl vdliweerps ppsrpldsls iiytgkfwqe
      181 kvadirqdms qksasvlvlt aldeiawlfn lrgsdidynp vffayavvtm dsaylfiden
      241 klsatlqrhl stdrneknlt vdirpyrvfk dflsllinqq sgkfwvssss syavvsqvpk
      301 erriesttpv mlrkaiknet eiecmrrahi kdavalceff vwmesevpkg evtemtaaak
      361 lehfrreqed yvgpsfetis asgpnaaiih yrpeeesdrr vtteeiylcd sggqyrdgtt
      421 dvtrtwhfgt psqyekecft rvvkgnials saifprlvkg qmldtlarra lwevgldylh
      481 gtghgvgafl nvhegpmgis wmphpndpgl qegmilsiep gyyedgqfgi rienlalvrk
      541 aatkynfkdr gflafdsltl vpiqtkmlnp lmltaeevew ldtyhqacrd vigraleeqg
      601 rdvalqwllr etqplg
//
LOCUS       XP_077501067             616 aa            linear   INV 25-NOV-2025
DEFINITION  aminopeptidase P isoform X3 [Amblyomma americanum].
ACCESSION   XP_077501067
VERSION     XP_077501067.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644941.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..616
                     /product="aminopeptidase P isoform X3"
                     /calculated_mol_wt=69438
     Region          10..137
                     /region_name="Creatinase_N"
                     /note="Creatinase/Prolidase N-terminal domain; pfam01321"
                     /db_xref="CDD:460159"
     Region          157..321
                     /region_name="Creatinase_N_2"
                     /note="Creatinase/Prolidase N-terminal domain; pfam16189"
                     /db_xref="CDD:465053"
     Region          325..548
                     /region_name="APP"
                     /note="X-Prolyl Aminopeptidase 2. E.C. 3.4.11.9. Also
                     known as X-Pro aminopeptidase, proline aminopeptidase,
                     aminopeptidase P, and aminoacylproline aminopeptidase.
                     Catalyses release of any N-terminal amino acid, including
                     proline, that is linked with proline; cd01085"
                     /db_xref="CDD:238518"
     Site            order(390,410,421,488,519,533)
                     /site_type="active"
                     /db_xref="CDD:238518"
     Region          551..615
                     /region_name="Peptidase_M24_C"
                     /note="C-terminal region of peptidase_M24; pfam16188"
                     /db_xref="CDD:465052"
     CDS             1..616
                     /gene="ApepP"
                     /coded_by="XM_077644941.1:738..2588"
                     /db_xref="GeneID:144111884"
ORIGIN      
        1 malrntgtll krlralmknt tyvsetiqay ivpsgdahqs eyiapcdkrr afltgftgsa
       61 gtaivtedha alwtdgryfl qaeqqldsnw vlmkdgipgt psqgewlckv lssgsrvgvd
      121 pflmpydawk qlsnqldvsg hclvpvaqnl vdliweerps ppsrpldsls iiytgkfwqe
      181 kvadirqdms qksasvlvlt aldeiawlfn lrgsdidynp vffayavvtm dsaylfiden
      241 klsatlqrhl stdrneknlt vdirpyrvfk dflsllinqq sgkfwvssss syavvsqvpk
      301 erriesttpv mlrkaiknet eiecmrrahi kdavalceff vwmesevpkg evtemtaaak
      361 lehfrreqed yvgpsfetis asgpnaaiih yrpeeesdrr vtteeiylcd sggqyrdgtt
      421 dvtrtwhfgt psqyekecft rvvkgnials saifprlvkg qmldtlarra lwevgldylh
      481 gtghgvgafl nvhegpmgis wmphpndpgl qegmilsiep gyyedgqfgi rienlalvrk
      541 aatkynfkdr gflafdsltl vpiqtkmlnp lmltaeevew ldtyhqacrd vigraleeqg
      601 rdvalqwllr etqplg
//
LOCUS       XP_077501071             616 aa            linear   INV 25-NOV-2025
DEFINITION  aminopeptidase P isoform X3 [Amblyomma americanum].
ACCESSION   XP_077501071
VERSION     XP_077501071.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644945.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..616
                     /product="aminopeptidase P isoform X3"
                     /calculated_mol_wt=69438
     Region          10..137
                     /region_name="Creatinase_N"
                     /note="Creatinase/Prolidase N-terminal domain; pfam01321"
                     /db_xref="CDD:460159"
     Region          157..321
                     /region_name="Creatinase_N_2"
                     /note="Creatinase/Prolidase N-terminal domain; pfam16189"
                     /db_xref="CDD:465053"
     Region          325..548
                     /region_name="APP"
                     /note="X-Prolyl Aminopeptidase 2. E.C. 3.4.11.9. Also
                     known as X-Pro aminopeptidase, proline aminopeptidase,
                     aminopeptidase P, and aminoacylproline aminopeptidase.
                     Catalyses release of any N-terminal amino acid, including
                     proline, that is linked with proline; cd01085"
                     /db_xref="CDD:238518"
     Site            order(390,410,421,488,519,533)
                     /site_type="active"
                     /db_xref="CDD:238518"
     Region          551..615
                     /region_name="Peptidase_M24_C"
                     /note="C-terminal region of peptidase_M24; pfam16188"
                     /db_xref="CDD:465052"
     CDS             1..616
                     /gene="ApepP"
                     /coded_by="XM_077644945.1:127..1977"
                     /db_xref="GeneID:144111884"
ORIGIN      
        1 malrntgtll krlralmknt tyvsetiqay ivpsgdahqs eyiapcdkrr afltgftgsa
       61 gtaivtedha alwtdgryfl qaeqqldsnw vlmkdgipgt psqgewlckv lssgsrvgvd
      121 pflmpydawk qlsnqldvsg hclvpvaqnl vdliweerps ppsrpldsls iiytgkfwqe
      181 kvadirqdms qksasvlvlt aldeiawlfn lrgsdidynp vffayavvtm dsaylfiden
      241 klsatlqrhl stdrneknlt vdirpyrvfk dflsllinqq sgkfwvssss syavvsqvpk
      301 erriesttpv mlrkaiknet eiecmrrahi kdavalceff vwmesevpkg evtemtaaak
      361 lehfrreqed yvgpsfetis asgpnaaiih yrpeeesdrr vtteeiylcd sggqyrdgtt
      421 dvtrtwhfgt psqyekecft rvvkgnials saifprlvkg qmldtlarra lwevgldylh
      481 gtghgvgafl nvhegpmgis wmphpndpgl qegmilsiep gyyedgqfgi rienlalvrk
      541 aatkynfkdr gflafdsltl vpiqtkmlnp lmltaeevew ldtyhqacrd vigraleeqg
      601 rdvalqwllr etqplg
//
LOCUS       XP_077501073             616 aa            linear   INV 25-NOV-2025
DEFINITION  aminopeptidase P isoform X3 [Amblyomma americanum].
ACCESSION   XP_077501073
VERSION     XP_077501073.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644947.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..616
                     /product="aminopeptidase P isoform X3"
                     /calculated_mol_wt=69438
     Region          10..137
                     /region_name="Creatinase_N"
                     /note="Creatinase/Prolidase N-terminal domain; pfam01321"
                     /db_xref="CDD:460159"
     Region          157..321
                     /region_name="Creatinase_N_2"
                     /note="Creatinase/Prolidase N-terminal domain; pfam16189"
                     /db_xref="CDD:465053"
     Region          325..548
                     /region_name="APP"
                     /note="X-Prolyl Aminopeptidase 2. E.C. 3.4.11.9. Also
                     known as X-Pro aminopeptidase, proline aminopeptidase,
                     aminopeptidase P, and aminoacylproline aminopeptidase.
                     Catalyses release of any N-terminal amino acid, including
                     proline, that is linked with proline; cd01085"
                     /db_xref="CDD:238518"
     Site            order(390,410,421,488,519,533)
                     /site_type="active"
                     /db_xref="CDD:238518"
     Region          551..615
                     /region_name="Peptidase_M24_C"
                     /note="C-terminal region of peptidase_M24; pfam16188"
                     /db_xref="CDD:465052"
     CDS             1..616
                     /gene="ApepP"
                     /coded_by="XM_077644947.1:139..1989"
                     /db_xref="GeneID:144111884"
ORIGIN      
        1 malrntgtll krlralmknt tyvsetiqay ivpsgdahqs eyiapcdkrr afltgftgsa
       61 gtaivtedha alwtdgryfl qaeqqldsnw vlmkdgipgt psqgewlckv lssgsrvgvd
      121 pflmpydawk qlsnqldvsg hclvpvaqnl vdliweerps ppsrpldsls iiytgkfwqe
      181 kvadirqdms qksasvlvlt aldeiawlfn lrgsdidynp vffayavvtm dsaylfiden
      241 klsatlqrhl stdrneknlt vdirpyrvfk dflsllinqq sgkfwvssss syavvsqvpk
      301 erriesttpv mlrkaiknet eiecmrrahi kdavalceff vwmesevpkg evtemtaaak
      361 lehfrreqed yvgpsfetis asgpnaaiih yrpeeesdrr vtteeiylcd sggqyrdgtt
      421 dvtrtwhfgt psqyekecft rvvkgnials saifprlvkg qmldtlarra lwevgldylh
      481 gtghgvgafl nvhegpmgis wmphpndpgl qegmilsiep gyyedgqfgi rienlalvrk
      541 aatkynfkdr gflafdsltl vpiqtkmlnp lmltaeevew ldtyhqacrd vigraleeqg
      601 rdvalqwllr etqplg
//
LOCUS       XP_077501085            1097 aa            linear   INV 25-NOV-2025
DEFINITION  atrial natriuretic peptide receptor 1-like [Amblyomma americanum].
ACCESSION   XP_077501085
VERSION     XP_077501085.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644959.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1097
                     /product="atrial natriuretic peptide receptor 1-like"
                     /calculated_mol_wt=123312
     Region          67..448
                     /region_name="PBP1_NPR_GC-like"
                     /note="ligand-binding domain of membrane guanylyl-cyclase
                     receptors; cd06352"
                     /db_xref="CDD:380575"
     Site            order(108,135..136)
                     /site_type="other"
                     /note="Cl binding site [ion binding]"
                     /db_xref="CDD:380575"
     Site            order(117,121,141,145,150,211..212,215,218)
                     /site_type="other"
                     /note="dimer interface (closed form) [polypeptide
                     binding]"
                     /db_xref="CDD:380575"
     Site            order(141,145,211..212,215,218..219,232)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:380575"
     Site            order(248..249,269,276)
                     /site_type="other"
                     /note="dimer interface (open form) [polypeptide binding]"
                     /db_xref="CDD:380575"
     Region          572..821
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Region          <832..880
                     /region_name="HNOBA"
                     /note="Heme NO binding associated; pfam07701"
                     /db_xref="CDD:462234"
     Region          859..1050
                     /region_name="CYCc"
                     /note="Adenylyl- / guanylyl cyclase, catalytic domain;
                     smart00044"
                     /db_xref="CDD:214485"
     Site            order(898,900..905,940,942..944,947,1012..1014,1018..1019,
                     1022..1023,1060)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(900,944)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(905,917,920..921,924,928,940..941,1000,1003,
                     1013..1016,1019,1060)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     CDS             1..1097
                     /gene="LOC144111914"
                     /coded_by="XM_077644959.1:35..3328"
                     /db_xref="GeneID:144111914"
ORIGIN      
        1 mrgwalvgav llsaavalpe ddslatpdyv elepgaepar nrtrwdsrfv dsegrflaki
       61 matfilskdl trsfdyriak paldiaitkv rklyphiafe ligrrgytsc innyagnyaa
      121 eeflsggvaa fvgpgcsmav dsvgrlasyw nvpvctaagv gaqfedrtiy gtlvrlalti
      181 nalrdaflqv lrhfgwrhla vlsdssqsff rqlrralegl fadpdtgvra vfrdfdstrs
      241 svnytsllve grsyarvfll agdgevirql lldaydlgmh dgqfafltae pykneryfge
      301 fswykegdpr ntevrkmyea lmilslkipt speykkfvrq vvdnsrkefn ttiedsivnv
      361 liagfydcvh lyawalnetl anggdpmdgr alidlimnst rygltgkihf nangdriaey
      421 alkaldpatl emqpvavyhg arqvlvpven atiywpgqrt epprdvpecg flgdaphcqr
      481 kesptllivv tlvtllsffg maivallfyr rvqletqlan fwwsikwedi sfpersrksa
      541 sslatndegl skgsprfgga cstqgcygdv hvgiykgmtv avktirmakf tatrtillel
      601 kqmrdvthen lvrlvgltte dpnvglvmey cprgslrdll eneslkldwt frysiindiv
      661 egmsflhggf iiyhgrlkst aclidarfvv kisnfglrel rkqvtppdie nprsllwtap
      721 ehlreevpgg svkgdvysfa iilqeiltrs gpfenlekys khflppeeil frvklgsrpp
      781 frpdvtpadc prellslmkl cwnespalrp sfailkqlmr kytrqmgssn lldnllqrme
      841 qyannleqlv gektaalvee krksdellye vlprsvaeql krgeyvqpea fecaticfsd
      901 ivgftaicah stpievvdll ndlyscfdai iinydvfkve tigdaymvvs glpvrngkeh
      961 areigrmtls lltaiagfri rhqpnsklrl rigvhsgpca agvvglkrpr yclfgdsvnt
     1021 asrmescgea mkihitretk rlldtfgtfr hrlrgdiyvk gkgmmrtyfl eaedppfspd
     1081 iglqgtfftn ehlmrql
//
LOCUS       XP_077501116             414 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111916 [Amblyomma americanum].
ACCESSION   XP_077501116
VERSION     XP_077501116.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077644990.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..414
                     /product="uncharacterized protein LOC144111916"
                     /calculated_mol_wt=47289
     Region          198..359
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..414
                     /gene="LOC144111916"
                     /coded_by="XM_077644990.1:1..1245"
                     /db_xref="GeneID:144111916"
ORIGIN      
        1 msddprderk feialamsvv lsssvdlals rrkvreirrr llannallia lassrrtvrr
       61 qvwayprner wfedtlpglg srhfrqsfrv setsfrylvd vcrpamerqi tnmrepipve
      121 krvavalykl cscaedrava dvfgigrstv nelyrefcet vvatlehewv kmptavdmra
      181 hirefeamcg fpqavgaldg chiavsppke hasdyynykg wysvillalv dhkyhfrfvs
      241 agspgrchds yvyqnstlar mvespifsap tatinrvivp plilcdqafp ltpnlvkpfr
      301 sldtcdghrs ynyhlskcrr vvenafgrlk arfrliskrm danvknvaqi iraccvlhni
      361 cehfndsvaq qwlheveidd avhvqpahss dvvtangadv realveyyqq feqh
//
LOCUS       XP_077501131             204 aa            linear   INV 25-NOV-2025
DEFINITION  microspherule protein Rcd5 [Amblyomma americanum].
ACCESSION   XP_077501131
VERSION     XP_077501131.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645005.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..204
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..204
                     /product="microspherule protein Rcd5"
                     /calculated_mol_wt=22958
     Region          <34..75
                     /region_name="MCRS_N"
                     /note="N-terminal region of micro-spherule protein;
                     pfam13325"
                     /db_xref="CDD:463845"
     Region          85..189
                     /region_name="FHA_MCRS1"
                     /note="forkhead associated (FHA) domain found in
                     microspherule protein 1 (MCRS1) and similar proteins;
                     cd22687"
                     /db_xref="CDD:438739"
     Site            order(110..111,132..133,135,152,154..155)
                     /site_type="other"
                     /note="putative phosphopeptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:438739"
     CDS             1..204
                     /gene="Rcd5"
                     /coded_by="XM_077645005.1:466..1080"
                     /db_xref="GeneID:144111917"
ORIGIN      
        1 msgdnvealp gtsrahsvap rkkkkekvrr twrkedlsgp qdevlehely lvdrnakrei
       61 rqlenelpkw qvlvdsvigv tpadfdnqtl avlrgrlvry lmrskeitig rvtkdnaidv
      121 dlslegpswk vsrrqgvikl rntgefiian egkrpiyidg kpvmagnkhk lnnnsvveia
      181 glrfvflvnq dlisvirsea pqss
//
LOCUS       XP_077501136             174 aa            linear   INV 25-NOV-2025
DEFINITION  TATA-box-binding protein-like [Amblyomma americanum].
ACCESSION   XP_077501136
VERSION     XP_077501136.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645010.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..174
                     /product="TATA-box-binding protein-like"
                     /calculated_mol_wt=19005
     Region          2..146
                     /region_name="TBP_TLF"
                     /note="TATA box binding protein (TBP): Present in archaea
                     and eukaryotes, TBPs are transcription factors that
                     recognize promoters and initiate transcription. TBP has
                     been shown to be an essential component of three different
                     transcription initiation complexes:...; cl08263"
                     /db_xref="CDD:447593"
     Site            order(13,20..22,24,29,35,37)
                     /site_type="other"
                     /note="TFIIA interaction surface [polypeptide binding]"
                     /db_xref="CDD:238352"
     Site            order(28..29,33,35,42,44,48,50,52,54,84,89,91,120..121,
                     126,137,139,141,145)
                     /site_type="other"
                     /note="DNA interaction surface [nucleotide binding]"
                     /db_xref="CDD:238352"
     Site            order(101..102,114..118,121,126)
                     /site_type="other"
                     /note="TFIIB interaction surface [polypeptide binding]"
                     /db_xref="CDD:238352"
     CDS             1..174
                     /gene="LOC144111921"
                     /coded_by="XM_077645010.1:257..781"
                     /db_xref="GeneID:144111921"
ORIGIN      
        1 maslslgcrl dlrkialhap nteydpkrtp alvmrirepr avplvfgsgk ilcsgakdem
       61 kaklasrkfa riiqklgfda hflnfkivni vascdvgfmi rleglllarr glasyepels
      121 pgltyrpehp rvtllifasg kivftgetsd nsclagkgss tlylchvanv wpmc
//
LOCUS       XP_077501138             174 aa            linear   INV 25-NOV-2025
DEFINITION  TATA-box-binding protein-like [Amblyomma americanum].
ACCESSION   XP_077501138
VERSION     XP_077501138.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645012.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..174
                     /product="TATA-box-binding protein-like"
                     /calculated_mol_wt=19005
     Region          2..146
                     /region_name="TBP_TLF"
                     /note="TATA box binding protein (TBP): Present in archaea
                     and eukaryotes, TBPs are transcription factors that
                     recognize promoters and initiate transcription. TBP has
                     been shown to be an essential component of three different
                     transcription initiation complexes:...; cl08263"
                     /db_xref="CDD:447593"
     Site            order(13,20..22,24,29,35,37)
                     /site_type="other"
                     /note="TFIIA interaction surface [polypeptide binding]"
                     /db_xref="CDD:238352"
     Site            order(28..29,33,35,42,44,48,50,52,54,84,89,91,120..121,
                     126,137,139,141,145)
                     /site_type="other"
                     /note="DNA interaction surface [nucleotide binding]"
                     /db_xref="CDD:238352"
     Site            order(101..102,114..118,121,126)
                     /site_type="other"
                     /note="TFIIB interaction surface [polypeptide binding]"
                     /db_xref="CDD:238352"
     CDS             1..174
                     /gene="LOC144111921"
                     /coded_by="XM_077645012.1:358..882"
                     /db_xref="GeneID:144111921"
ORIGIN      
        1 maslslgcrl dlrkialhap nteydpkrtp alvmrirepr avplvfgsgk ilcsgakdem
       61 kaklasrkfa riiqklgfda hflnfkivni vascdvgfmi rleglllarr glasyepels
      121 pgltyrpehp rvtllifasg kivftgetsd nsclagkgss tlylchvanv wpmc
//
LOCUS       XP_077501145             174 aa            linear   INV 25-NOV-2025
DEFINITION  TATA-box-binding protein-like [Amblyomma americanum].
ACCESSION   XP_077501145
VERSION     XP_077501145.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645019.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..174
                     /product="TATA-box-binding protein-like"
                     /calculated_mol_wt=19005
     Region          2..146
                     /region_name="TBP_TLF"
                     /note="TATA box binding protein (TBP): Present in archaea
                     and eukaryotes, TBPs are transcription factors that
                     recognize promoters and initiate transcription. TBP has
                     been shown to be an essential component of three different
                     transcription initiation complexes:...; cl08263"
                     /db_xref="CDD:447593"
     Site            order(13,20..22,24,29,35,37)
                     /site_type="other"
                     /note="TFIIA interaction surface [polypeptide binding]"
                     /db_xref="CDD:238352"
     Site            order(28..29,33,35,42,44,48,50,52,54,84,89,91,120..121,
                     126,137,139,141,145)
                     /site_type="other"
                     /note="DNA interaction surface [nucleotide binding]"
                     /db_xref="CDD:238352"
     Site            order(101..102,114..118,121,126)
                     /site_type="other"
                     /note="TFIIB interaction surface [polypeptide binding]"
                     /db_xref="CDD:238352"
     CDS             1..174
                     /gene="LOC144111921"
                     /coded_by="XM_077645019.1:99..623"
                     /db_xref="GeneID:144111921"
ORIGIN      
        1 maslslgcrl dlrkialhap nteydpkrtp alvmrirepr avplvfgsgk ilcsgakdem
       61 kaklasrkfa riiqklgfda hflnfkivni vascdvgfmi rleglllarr glasyepels
      121 pgltyrpehp rvtllifasg kivftgetsd nsclagkgss tlylchvanv wpmc
//
LOCUS       XP_077501150             174 aa            linear   INV 25-NOV-2025
DEFINITION  TATA-box-binding protein-like [Amblyomma americanum].
ACCESSION   XP_077501150
VERSION     XP_077501150.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645024.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..174
                     /product="TATA-box-binding protein-like"
                     /calculated_mol_wt=19005
     Region          2..146
                     /region_name="TBP_TLF"
                     /note="TATA box binding protein (TBP): Present in archaea
                     and eukaryotes, TBPs are transcription factors that
                     recognize promoters and initiate transcription. TBP has
                     been shown to be an essential component of three different
                     transcription initiation complexes:...; cl08263"
                     /db_xref="CDD:447593"
     Site            order(13,20..22,24,29,35,37)
                     /site_type="other"
                     /note="TFIIA interaction surface [polypeptide binding]"
                     /db_xref="CDD:238352"
     Site            order(28..29,33,35,42,44,48,50,52,54,84,89,91,120..121,
                     126,137,139,141,145)
                     /site_type="other"
                     /note="DNA interaction surface [nucleotide binding]"
                     /db_xref="CDD:238352"
     Site            order(101..102,114..118,121,126)
                     /site_type="other"
                     /note="TFIIB interaction surface [polypeptide binding]"
                     /db_xref="CDD:238352"
     CDS             1..174
                     /gene="LOC144111921"
                     /coded_by="XM_077645024.1:199..723"
                     /db_xref="GeneID:144111921"
ORIGIN      
        1 maslslgcrl dlrkialhap nteydpkrtp alvmrirepr avplvfgsgk ilcsgakdem
       61 kaklasrkfa riiqklgfda hflnfkivni vascdvgfmi rleglllarr glasyepels
      121 pgltyrpehp rvtllifasg kivftgetsd nsclagkgss tlylchvanv wpmc
//
LOCUS       XP_077501169             541 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111943 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501169
VERSION     XP_077501169.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645043.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..541
                     /product="uncharacterized protein LOC144111943 isoform X1"
                     /calculated_mol_wt=61035
     Region          461..480
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(465,467,469,471..472,475..476,479,495,497,501..502,
                     505..506,509,523,525,527,529..530,533..534,537)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          <472..538
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          488..510
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          518..538
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..541
                     /gene="LOC144111943"
                     /coded_by="XM_077645043.1:243..1868"
                     /db_xref="GeneID:144111943"
ORIGIN      
        1 mrvqdhfkmr llnfeehcal tatnepvass rwvievikpf qmpgaaarne ssrvpgngrp
       61 hhavepasqq vlrfeeemat kgyyswtpay fsdetwrkll ddikmryttv rkdgsikerk
      121 qattnrpflr krcpgyapaa klgpssdepe ivvlddsssc dvvfkthnes ppsspdsvil
      181 vkwekgiksc rpdleqegas eewlrceqrv ptgeeepmvv epselqdqrq laegldsivd
      241 gsqheanvpa tqllpmntkh dliqcenrey rdlqgdqqhh itqgnqetsg qlqdqrqpae
      301 gldsivdgsq heanvpatql lpmntehdli qcenrecrdl qgdqqhlikq gnqetsgqfs
      361 dqrqpaegld piqcenrecr dlhgdqqhhi kqqtpphvdt vtrfcssteg qsastaigsa
      421 nletgprcss avlavpegke pggqqgttrv kggavhicpy pdcgrrhtkr rhfeahlrsh
      481 tgekpyecgw ancgrkfars daltrhyrkh tgerlylcew cgrtfsrndn llrhqkthrg
      541 e
//
LOCUS       XP_077501173             500 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111943 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501173
VERSION     XP_077501173.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645047.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..500
                     /product="uncharacterized protein LOC144111943 isoform X2"
                     /calculated_mol_wt=56180
     Region          420..439
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(424,426,428,430..431,434..435,438,454,456,460..461,
                     464..465,468,482,484,486,488..489,492..493,496)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          <431..497
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          447..469
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          477..497
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..500
                     /gene="LOC144111943"
                     /coded_by="XM_077645047.1:4923..6425"
                     /db_xref="GeneID:144111943"
ORIGIN      
        1 mpgaaarnes srvpgngrph havepasqqv lrfeeematk gyyswtpayf sdetwrklld
       61 dikmryttvr kdgsikerkq attnrpflrk rcpgyapaak lgpssdepei vvlddssscd
      121 vvfkthnesp psspdsvilv kwekgikscr pdleqegase ewlrceqrvp tgeeepmvve
      181 pselqdqrql aegldsivdg sqheanvpat qllpmntkhd liqcenreyr dlqgdqqhhi
      241 tqgnqetsgq lqdqrqpaeg ldsivdgsqh eanvpatqll pmntehdliq cenrecrdlq
      301 gdqqhlikqg nqetsgqfsd qrqpaegldp iqcenrecrd lhgdqqhhik qqtpphvdtv
      361 trfcsstegq sastaigsan letgprcssa vlavpegkep ggqqgttrvk ggavhicpyp
      421 dcgrrhtkrr hfeahlrsht gekpyecgwa ncgrkfarsd altrhyrkht gerlylcewc
      481 grtfsrndnl lrhqkthrge
//
LOCUS       XP_077501178             474 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111943 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501178
VERSION     XP_077501178.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645052.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..474
                     /product="uncharacterized protein LOC144111943 isoform X3"
                     /calculated_mol_wt=53546
     Region          394..413
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(398,400,402,404..405,408..409,412,428,430,434..435,
                     438..439,442,456,458,460,462..463,466..467,470)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          <405..471
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          421..443
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          451..471
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..474
                     /gene="LOC144111943"
                     /coded_by="XM_077645052.1:243..1667"
                     /db_xref="GeneID:144111943"
ORIGIN      
        1 mrvqdhfkmr llnfeehcal tatnepvass rwvievikpf qmpgaaarne ssrvpgngrp
       61 hhavepasqq vlrfeeemat kgyyswtpay fsdetwrkll ddikmryttv rkdgsikerk
      121 qattnrpflr krcpgyapaa klgpssdepe ivvlddsssc dvvfkthnes ppsspdsvil
      181 vkwekgiksc rpdleqegas eewlrceqrv ptgeeepmvv epselqdqrq laegldsivd
      241 gsqheanvpa tqllpmntkh dliqcenrey rdlqgdqqhh itqgnqetsg qfsdqrqpae
      301 gldpiqcenr ecrdlhgdqq hhikqqtpph vdtvtrfcss tegqsastai gsanletgpr
      361 cssavlavpe gkepggqqgt trvkggavhi cpypdcgrrh tkrrhfeahl rshtgekpye
      421 cgwancgrkf arsdaltrhy rkhtgerlyl cewcgrtfsr ndnllrhqkt hrge
//
LOCUS       XP_077501184             332 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111965 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501184
VERSION     XP_077501184.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645058.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..332
                     /product="uncharacterized protein LOC144111965 isoform X1"
                     /calculated_mol_wt=37800
     CDS             1..332
                     /gene="LOC144111965"
                     /coded_by="XM_077645058.1:49..1047"
                     /db_xref="GeneID:144111965"
ORIGIN      
        1 mavvcvdavs aqnaaafrda tvslqllhrp phlehsgpac srlqvheega pmwrvrgrrl
       61 ptraqplder ellaqqaqtk hwglalqwrh lpkeehhsse qleamsaqlt keygsgrwaa
      121 nntcaafwlf qeentkdkik nedvniiplh slflacekrg dlfstlynle vhtrrkhwhs
      181 vhgihrctyc tytstrkcpe lqrgtravec lpfyplhlrs rmgvlateyr ilqwnqprek
      241 cgalrkdyvt gkqflpivgp kaiqvqlfea fqgapnswis nvsgisasgf stvecvaprt
      301 gstneeqevp cprqsceeee mateeyyrwk pa
//
LOCUS       XP_077501188             263 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111965 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501188
VERSION     XP_077501188.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645062.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..263
                     /product="uncharacterized protein LOC144111965 isoform X2"
                     /calculated_mol_wt=29836
     CDS             1..263
                     /gene="LOC144111965"
                     /coded_by="XM_077645062.1:49..840"
                     /db_xref="GeneID:144111965"
ORIGIN      
        1 mavvcvdavs aqnaaafrda tvslqllhrp phlehsgpac srlqvheega pmwrvrgrrl
       61 ptraqplder ellaqqaqtk hwglalqwrh lpkeedvnii plhslflace krgdlfstly
      121 nlevhtrrkh whsvhgihrc tyctytstrk srmgvlatey rilqwnqpre kcgalrkdyv
      181 tgkqflpivg pkaiqvqlfe afqgapnswi snvsgisasg fstvecvapr tgstneeqev
      241 pcprqsceee emateeyyrw kpa
//
LOCUS       XP_077501200             172 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144111982 [Amblyomma americanum].
ACCESSION   XP_077501200
VERSION     XP_077501200.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645074.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..172
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..172
                     /product="uncharacterized protein LOC144111982"
                     /calculated_mol_wt=18552
     Region          26..>101
                     /region_name="DEAD-like_helicase_N"
                     /note="N-terminal helicase domain of the DEAD-box helicase
                     superfamily; cl28899"
                     /db_xref="CDD:475120"
     Site            order(55..58,61..63)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:350670"
     CDS             1..172
                     /gene="LOC144111982"
                     /coded_by="XM_077645074.1:2944..3462"
                     /db_xref="GeneID:144111982"
ORIGIN      
        1 mplqrnnaql ripcassedv cpcfmllqnl rgamhveppp iqdyasatgl dcpqagsaaf
       61 ktalphtlvn esasgdpscl plrtflaail sptrrlpiqm htyaqeltvn avlvkdgggp
      121 agkshpgrwt ssraarvhrg rfssvcctkp twwcrgfapn tryhtsmltr gp
//
LOCUS       XP_077501206            4588 aa            linear   INV 25-NOV-2025
DEFINITION  low-density lipoprotein receptor-related protein megalin isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077501206
VERSION     XP_077501206.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645080.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..4588
                     /product="low-density lipoprotein receptor-related protein
                     megalin isoform X1"
                     /calculated_mol_wt=512669
     Region          34..66
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(40,48,59..60)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(52,55,59,65..66)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            62..66
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          75..113
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(80,93,104..105)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(97,100,104,110..111)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            107..111
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          120..154
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(125,133,144..145)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(137,140,144,150..151)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            147..151
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          161..196
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(166,175,186..187)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(179,182,186,192..193)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            189..193
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          201..235
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(206,214,225..226)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(218,221,225,231..232)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            228..232
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          240..276
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(245,254,265..266)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(258,261,265,271..272)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            268..272
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          317..355
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(317,320,333)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          <371..>516
                     /region_name="YncE"
                     /note="DNA-binding beta-propeller fold protein YncE
                     [General function prediction only]; COG3391"
                     /db_xref="CDD:442618"
     Region          470..509
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          627..669
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     Region          <712..741
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          786..829
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          814..>939
                     /region_name="YncE"
                     /note="DNA-binding beta-propeller fold protein YncE
                     [General function prediction only]; COG3391"
                     /db_xref="CDD:442618"
     Region          837..872
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          945..988
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     Region          1007..1035
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1007,1015,1026..1027)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1019,1022,1026,1032..1033)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1029..1033
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1042..1076
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1047,1055,1066..1067)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1059,1062,1066,1072..1073)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1069..1073
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1080..1112
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(1086,1094,1105..1106)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1098,1101,1105,1111..1112)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1108..1112
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1121..1156
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1126,1135,1146..1147)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1139,1142,1146,1152..1153)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1149..1153
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1161..1196
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1166,1175,1186..1187)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1179,1182,1186,1192..1193)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1189..1193
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1210..1237
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(1210,1219,1230..1231)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1223,1226,1230,1236..1237)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1233..1237
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1245..1277
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(1251,1259,1270..1271)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1263,1266,1270,1276..1277)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1273..1277
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1283..1323
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(1295,1305,1316..1317)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1309,1312,1316,1322..1323)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1319..1323
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1475..1517
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          1540..1580
                     /region_name="Ldl_recept_b"
                     /note="Low-density lipoprotein receptor repeat class B;
                     pfam00058"
                     /db_xref="CDD:459654"
     Region          1564..1606
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          1765..>1952
                     /region_name="YvrE"
                     /note="Sugar lactone lactonase YvrE [Carbohydrate
                     transport and metabolism]; COG3386"
                     /db_xref="CDD:442613"
     Region          2114..2160
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          2181..2222
                     /region_name="Ldl_recept_b"
                     /note="Low-density lipoprotein receptor repeat class B;
                     pfam00058"
                     /db_xref="CDD:459654"
     Region          2207..2247
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          2402..>2589
                     /region_name="YvrE"
                     /note="Sugar lactone lactonase YvrE [Carbohydrate
                     transport and metabolism]; COG3386"
                     /db_xref="CDD:442613"
     Region          2515..2557
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          2711..2745
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(2716,2724,2735..2736)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2728,2731,2735,2741..2742)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2738..2742
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2750..2785
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(2755,2763,2774..2775)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2767,2770,2774,2782..2783)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            order(2777..2778,2781..2783)
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2793..2826
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(2799,2808,2819..2820)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2812,2815,2819,2825..2826)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2822..2826
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2878..2910
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(2884,2892,2903..2904)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2896,2899,2903,2909..2910)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2906..2910
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2926..2961
                     /region_name="Ldl_recept_a"
                     /note="Low-density lipoprotein receptor domain class A;
                     pfam00057"
                     /db_xref="CDD:395011"
     Site            order(2927,2941,2952..2953)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2945,2948,2952,2958..2959)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2955..2959
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2974..3004
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(2978,2986,2997..2998)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2990,2993,2997,3003..3004)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3000..3004
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3013..3044
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(3018,3026,3037..3038)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3030,3033,3037,3043..3044)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3040..3044
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3055..3087
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(3061,3069,3080..3081)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3073,3076,3080,3086..3087)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3083..3087
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          <3093..3131
                     /region_name="vWFA"
                     /note="Von Willebrand factor type A (vWA) domain was
                     originally found in the blood coagulation protein von
                     Willebrand factor (vWF). Typically, the vWA domain is made
                     up of approximately 200 amino acid residues folded into a
                     classic a/b para-rossmann type of...; cl00057"
                     /db_xref="CDD:469594"
     Region          <3129..3164
                     /region_name="vWFA"
                     /note="Von Willebrand factor type A (vWA) domain was
                     originally found in the blood coagulation protein von
                     Willebrand factor (vWF). Typically, the vWA domain is made
                     up of approximately 200 amino acid residues folded into a
                     classic a/b para-rossmann type of...; cl00057"
                     /db_xref="CDD:469594"
     Region          <3214..3404
                     /region_name="Vgb"
                     /note="Streptogramin lyase [Defense mechanisms]; COG4257"
                     /db_xref="CDD:443399"
     Region          3245..3287
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          3335..3376
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          3447..3484
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     Region          3488..3524
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3493,3503,3514..3515)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3507,3510,3514,3520..3521)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3517..3521
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3529..3564
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3534,3543,3554..3555)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3547,3550,3554,3560..3561)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3557..3561
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3568..3601
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3573,3582,3593..3594)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3586,3589,3593,3599..3600)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3596..3600
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3612..3642
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3616,3624,3635..3636)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3628,3631,3635,3641..3642)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3638..3642
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3652..3686
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(3657,3668,3679..3680)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3672,3675,3679,3685..3686)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3682..3686
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3694..3727
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(3701,3709,3720..3721)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3713,3716,3720,3726..3727)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3723..3727
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3739..3773
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3744,3752,3763..3764)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3756,3759,3763,3769..3770)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3766..3770
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3778..3812
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3783,3791,3802..3803)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3795,3798,3802,3808..3809)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3805..3809
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3868..3897
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3869,3877,3888..3889)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3881,3884,3888,3894..3895)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3891..3895
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3909..3943
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3914,3922,3933..3934)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3926,3929,3933,3939..3940)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3936..3940
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3989..4018
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF domain; pfam07645"
                     /db_xref="CDD:429571"
     Region          4056..4090
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          4110..4152
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          4153..4196
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          4197..4240
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          4315..4338
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     CDS             1..4588
                     /gene="mgl"
                     /coded_by="XM_077645080.1:69..13835"
                     /db_xref="GeneID:144111991"
ORIGIN      
        1 mgfsdnrrvl lgtvcvllcv tsasdsaeve etttcstdnf rcsngrcipl rwvcdyqkdc
       61 eegedehqsc appqcnsnqf scgqyvfnqs ycipkhwrcd qvvdcvdgtd esgdckyrvc
      121 eetdhkcgsg lcvpaskkcd gyydcrdesd eascttngta crlnefrcqh gnkcideskk
      181 cdhwddcgdn sdeegcdfpp cnegqfrctn aicipirwrc dghsdcsdqs dernctsvtc
      241 ldtkflcpse kkcidksklc dgkqdcddga dekkncsssl crtlscehgc rasleggdcf
      301 cppgmtintk dkrscmdlne csewgycdql ctnspgsyqc lcvrgytldp prhckadnss
      361 amrllftrhn aiysvssdga dlsilanass atgldyhhdr kllfwtdtet kqihsmrlag
      421 dkelrspisv vfswnpvsma vdwvgnklyv cdslgqkidl lemdglrhai vlsrnlsnpq
      481 dialdptvgy mffsdgdhid rahmdgshrt tivtaiykat gltvdyvtkf lvwcdsqldq
      541 ivaidytgsn rniiirgstr vpspnkitlf edkvywtdgt rqgvlqarif nnndtvtivh
      601 rdrqlskepg aiksyhplrq pvvsnpcgln nggcqhmciv trgsggfgll gyrcvchtgf
      661 ellpnrkdct rvstfllysq qkfvrgslmn itqdgftdam vpivsksarf vgldfdarer
      721 fiyysdvild viyrvtidgs gkenvlasqn egveglaldw vshnlyyids rkgtlnvisv
      781 knfqyrrtll knlkrpraiv vhpnkgyvfy sewdrpanis rayldgsnvm vfrgvllgwp
      841 nglsidfqtd rlywcdalld hiqhanldgt dvqtinspri khpfslvihg dslfvtdwrl
      901 dailkmhkit gadekiittv eegsrlygik vysrdnqkvd srhpcqfnng gcqklcfpvp
      961 sngsqgpdlv vscgcpygeq lskdnrtcis dpsaeppvqa cpntwdftce nqrcipktwv
     1021 cdgdddcldn sdemnnctrn tcsprefrcg sgrciplsfk cdsdndcgdf sdetgctnvk
     1081 cdnsefscen grcipqswkc dsendcgdgs degdfckekt cayyqftcpg sghciprswv
     1141 cdgdndcfdq adenncppav csssqfqcsn kkqcihesyr cdgvsdcqdg sdeqgcpsla
     1201 pdqcdleksf qckksrvcip klwycdgnad cedgsdepss cgqvecpqnh fkcnnskcif
     1261 kswicdgsdd cgdnsdedar hacvapkfqc ehgqwlcpgv tdrcvnislv cdgkpdcpng
     1321 adegpgcast pckgnkagct hgchvtpmgp lctcpkgevl ndtttcvdln ecliagtcsq
     1381 hctnskgsft caceqgytle lkhkckatnr sdaflvisnr rsilvanldt sflervpvkv
     1441 envvatasdm atntiywsdm svkkvfrltk ggepqvviss gldlveglav dwvarnlywv
     1501 dsrlktievs tldgknhivl lkenisqprg lsldpregvr lmfwtdwgen prvesvgldg
     1561 tmrktivhtk iywpngltld lpnkriyfad skldyidfcn ydgsgrqqvl ahnhyllhph
     1621 sltifedtly wtdrqlnrvl scqkfrgtnq tvvshlvsqp lgihvnhpvl qppssnpcat
     1681 apcshlclls psrpsqyscr cppgytqdra sasgscvpvd tpflmlmkan qlidisltpd
     1741 dkssgqivpi igiengydfd ydkqegniyw iqladedken gtiytvslkg gnvtkflmdg
     1801 iigapyaiaf dwvgrnlyig nrkssnieiv kvdgekqyrr vlltndgsek gigkpkaivv
     1861 dpeqgklfwl ddggigvprk larvsmdgkn piilvkenlr rlealtidlt gkrvywsqsf
     1921 egiiesvdld gngrktliss taglakpqgl tvfsnklyyl dsvhekiarv dlpdggnqvv
     1981 ledntpglvt lkvygkkpge gdshpcrsnn ggceqlcipv snnqrqcmcs tgykgngdts
     2041 cmpyssflvv seldkargfs ledhgeamqp iagpghnilh ldvhvamnhi ywiefnqger
     2101 ngiyrikpdg tdlkhviadg igsngirgva vdwvagniyf tnvfphetyi evceldgsnr
     2161 villktttds preiavnpvk rflywidygq ypkiekayld gtnrvpivvt gisnprdlti
     2221 divthdvywv dsredavqkv afsggnrqyv rknlpnpmgv avsgqyvywa drnlrtiyka
     2281 skfqtntstp pepvksdlnm lrdivvfdss nqppasspcs trpcdqlcfs mpesyngtkw
     2341 kcgcasgela adgrkcdmsk vflvfttrre irslfmdphk tsvpfqprgn ltnvvgldfd
     2401 yadkrmyftq irpdgsiswv npdkmdihvv lnktinpegi afdwtakkiy wtdsanrsiy
     2461 amnrdgtqlv miahverpra ivldpcqgym yytdwgrygn tgkiyratma gnnkqaiveq
     2521 nltqpsglai dyddkklywt dalrekiers dldgknretl vsatiypfai tvyenyiywt
     2581 dlqlrgvyra ekhtgagmie mvkrleespr dihvyspdrq kcdknpcslq nggcahschq
     2641 apngtieclc nstfkvaneg rmcvpenvsc ddtkfacgng kciprlwacd gdddcgdntd
     2701 edksfcaqht cgptefrcgn grcifktwkc dhendcgdst deegceyppc pdgeftcanf
     2761 rcipktqlcn gvndckdnkt sdeshancpn nrtcpgnhlk cettnicvep ywlcdgdndc
     2821 gdnsdevall csqrtcppns frcpnhrcip atwhcdgddd cgdkadeped ycksekrtcf
     2881 gdlftcdngn cvpriyicdg dndcldnsde dvrhqcdtre cdaerefkct qnkqwgratc
     2941 ipkrwvcdgd pdcvdgaden ttahncpape pcdpekqyqc knqrcinkdw vcdhdndcgd
     3001 gsdeprnctf rnctseeftc rnakcirksy hcdgeddcgd gsdelekdck keettcsgaq
     3061 frcksgqcip yervcnkqqd csdgsdepah cnvdecakve lnqcehkcvn tltsfycecn
     3121 dgyellkdgk acadinecqv kpavcsqycv nspgsyfckc nqtyydrefd ghtckrkddi
     3181 tpwlifsnry ylrntsldgt lynlvkmnlr nvvaidfdyr egriyfadvg nktiqriytn
     3241 gtgeqvlvrh dvhgleglav dwigrklywl drtskqmyva eldgtsrktl lpkgisdpra
     3301 vavhpgigyl fftdwghhaf igrlgldgtn ftrivlyenk lvwpnaltid yfsdklfwad
     3361 ahldyiefsd ldgknrhtvl qgrtvphvfa ltvfddwlfw tdwnmkgvyr ahkftgeklq
     3421 vlrntshrpy dihvyhplrq lpypnpcgtn nggcshlcli spgassfrca cpdnfvllpd
     3481 ektciancte gqfrcggsdd rcisvfwkcd gekdcrdgsd evgcpafsck kgqfqchnna
     3541 tcvprikicd ghndcgdqsd ekfcgypcge hnfkcqntgr cvpvswqcdg dndctdgsde
     3601 dpaichhqec ntdtqykcnn gkcipklwyc dfdddcgdns depahkcrnr ncttgwqkcp
     3661 svnnyrcipt wlfcdgkddc rdnsdetspd ycpkchetgd fkcrnnrcip lrwrcdfedd
     3721 cgdnsdedst mcaelyrdcs esefqcankk cipsrwrcdh dndcddgsde kdclnyecke
     3781 nqfkcnsghc ilsrlvcdgn kdchdvsdeq ncptrvpggl ycqenmfqcn ntvcirpdfm
     3841 cdgdddcgdf sdesdsickd hkcdvtrkfq cqnnkciplw qlcngnddcg dgsdennhql
     3901 cgkltplpcl atefkctngk cipraevcnh kddcgdlsde rgchegtcda atrggcehnc
     3961 ttlgdsngyi ctcasgykik dddpkrcedi necatfghnc sqicenlmgd ygcrckegye
     4021 mfkshchakg papivlytng pdirmldmkn evqssvitge srvqaldydp rehmlywtds
     4081 yektikrafl pnltdvsagi gfpqnlelkg ltkpnaiavd wvgrtiywmd adlsstrpkg
     4141 rifvslidgr yrktllssnl erptslavdp dmgwmfwsds gsnpkietaw mdgskrriiv
     4201 gdkigyptgl tidyasdhri ywcdsklnti etakqdgsnr vvivsgdlmh pfamdlfeds
     4261 ifwvtrdsge iyrqdkhgrg vkvrvkrnve nptgvkifqp lkynltvpnr cpssgcshlc
     4321 llvpqgyrca cpdgtslhvl nfgscdagfe vpkpqpykcp crnggrcesv rdeqivcrcs
     4381 seyegmhcen fiarrrissl ggasgvaavl vpiivvliim ivaatlfvcv rrrhfkqsgl
     4441 ggstqsvsfr sgtnvefspn fmrtrqaetn aesldadfgl ndvnkatdfs npmydalgna
     4501 dgtagagetg kgglyevagd vfdkkydknc fdssnkapps vgsavlhpsm vlhrssphvp
     4561 lrqtaldptt vdtdkdtqnl veedksec
//
LOCUS       XP_077501211            4588 aa            linear   INV 25-NOV-2025
DEFINITION  low-density lipoprotein receptor-related protein megalin isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077501211
VERSION     XP_077501211.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645085.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..4588
                     /product="low-density lipoprotein receptor-related protein
                     megalin isoform X2"
                     /calculated_mol_wt=512642
     Region          34..66
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(40,48,59..60)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(52,55,59,65..66)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            62..66
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          74..111
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(80,93,104..105)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(97,100,104,110..111)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            107..111
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          120..154
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(125,133,144..145)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(137,140,144,150..151)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            147..151
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          161..196
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(166,175,186..187)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(179,182,186,192..193)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            189..193
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          201..235
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(206,214,225..226)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(218,221,225,231..232)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            228..232
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          240..276
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(245,254,265..266)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(258,261,265,271..272)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            268..272
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          317..355
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(317,320,333)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          <371..>516
                     /region_name="YncE"
                     /note="DNA-binding beta-propeller fold protein YncE
                     [General function prediction only]; COG3391"
                     /db_xref="CDD:442618"
     Region          470..509
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          627..669
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     Region          <712..741
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          786..829
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          814..>939
                     /region_name="YncE"
                     /note="DNA-binding beta-propeller fold protein YncE
                     [General function prediction only]; COG3391"
                     /db_xref="CDD:442618"
     Region          837..872
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          945..988
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     Region          1007..1035
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1007,1015,1026..1027)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1019,1022,1026,1032..1033)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1029..1033
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1042..1076
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1047,1055,1066..1067)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1059,1062,1066,1072..1073)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1069..1073
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1080..1112
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(1086,1094,1105..1106)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1098,1101,1105,1111..1112)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1108..1112
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1121..1156
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1126,1135,1146..1147)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1139,1142,1146,1152..1153)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1149..1153
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1161..1196
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1166,1175,1186..1187)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1179,1182,1186,1192..1193)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1189..1193
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1210..1237
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(1210,1219,1230..1231)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1223,1226,1230,1236..1237)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1233..1237
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1245..1277
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(1251,1259,1270..1271)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1263,1266,1270,1276..1277)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1273..1277
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1283..1323
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(1295,1305,1316..1317)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1309,1312,1316,1322..1323)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1319..1323
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1475..1517
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          1540..1580
                     /region_name="Ldl_recept_b"
                     /note="Low-density lipoprotein receptor repeat class B;
                     pfam00058"
                     /db_xref="CDD:459654"
     Region          1564..1606
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          1765..>1952
                     /region_name="YvrE"
                     /note="Sugar lactone lactonase YvrE [Carbohydrate
                     transport and metabolism]; COG3386"
                     /db_xref="CDD:442613"
     Region          2114..2160
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          2181..2222
                     /region_name="Ldl_recept_b"
                     /note="Low-density lipoprotein receptor repeat class B;
                     pfam00058"
                     /db_xref="CDD:459654"
     Region          2207..2247
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          2402..>2589
                     /region_name="YvrE"
                     /note="Sugar lactone lactonase YvrE [Carbohydrate
                     transport and metabolism]; COG3386"
                     /db_xref="CDD:442613"
     Region          2515..2557
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          2711..2745
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(2716,2724,2735..2736)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2728,2731,2735,2741..2742)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2738..2742
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2750..2785
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(2755,2763,2774..2775)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2767,2770,2774,2782..2783)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            order(2777..2778,2781..2783)
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2793..2826
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(2799,2808,2819..2820)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2812,2815,2819,2825..2826)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2822..2826
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2878..2910
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(2884,2892,2903..2904)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2896,2899,2903,2909..2910)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2906..2910
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2926..2961
                     /region_name="Ldl_recept_a"
                     /note="Low-density lipoprotein receptor domain class A;
                     pfam00057"
                     /db_xref="CDD:395011"
     Site            order(2927,2941,2952..2953)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2945,2948,2952,2958..2959)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2955..2959
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2974..3004
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(2978,2986,2997..2998)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2990,2993,2997,3003..3004)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3000..3004
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3013..3044
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(3018,3026,3037..3038)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3030,3033,3037,3043..3044)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3040..3044
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3055..3087
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(3061,3069,3080..3081)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3073,3076,3080,3086..3087)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3083..3087
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          <3093..3131
                     /region_name="vWFA"
                     /note="Von Willebrand factor type A (vWA) domain was
                     originally found in the blood coagulation protein von
                     Willebrand factor (vWF). Typically, the vWA domain is made
                     up of approximately 200 amino acid residues folded into a
                     classic a/b para-rossmann type of...; cl00057"
                     /db_xref="CDD:469594"
     Region          <3129..3164
                     /region_name="vWFA"
                     /note="Von Willebrand factor type A (vWA) domain was
                     originally found in the blood coagulation protein von
                     Willebrand factor (vWF). Typically, the vWA domain is made
                     up of approximately 200 amino acid residues folded into a
                     classic a/b para-rossmann type of...; cl00057"
                     /db_xref="CDD:469594"
     Region          <3214..3404
                     /region_name="Vgb"
                     /note="Streptogramin lyase [Defense mechanisms]; COG4257"
                     /db_xref="CDD:443399"
     Region          3245..3287
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          3335..3376
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          3447..3484
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     Region          3488..3524
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3493,3503,3514..3515)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3507,3510,3514,3520..3521)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3517..3521
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3529..3564
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3534,3543,3554..3555)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3547,3550,3554,3560..3561)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3557..3561
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3568..3601
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3573,3582,3593..3594)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3586,3589,3593,3599..3600)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3596..3600
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3612..3642
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3616,3624,3635..3636)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3628,3631,3635,3641..3642)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3638..3642
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3652..3686
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(3657,3668,3679..3680)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3672,3675,3679,3685..3686)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3682..3686
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3694..3727
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(3701,3709,3720..3721)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3713,3716,3720,3726..3727)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3723..3727
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3739..3773
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3744,3752,3763..3764)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3756,3759,3763,3769..3770)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3766..3770
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3778..3812
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3783,3791,3802..3803)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3795,3798,3802,3808..3809)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3805..3809
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3868..3897
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3869,3877,3888..3889)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3881,3884,3888,3894..3895)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3891..3895
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3909..3943
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3914,3922,3933..3934)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3926,3929,3933,3939..3940)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3936..3940
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3989..4018
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF domain; pfam07645"
                     /db_xref="CDD:429571"
     Region          4056..4090
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          4110..4152
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          4153..4196
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          4197..4240
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          4315..4338
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     CDS             1..4588
                     /gene="mgl"
                     /coded_by="XM_077645085.1:69..13835"
                     /db_xref="GeneID:144111991"
ORIGIN      
        1 mgfsdnrrvl lgtvcvllcv tsasdsaeve etttcstdnf rcsngrcipl rwvcdyqkdc
       61 eegedehqsc appqcnsnqf scgqyvfnqs ycipkhwrcd qvvdcvdgtd esgdckyrvc
      121 eetdhkcgsg lcvpaskkcd gyydcrdesd eascttngta crlnefrcqh gnkcideskk
      181 cdhwddcgdn sdeegcdfpp cnegqfrctn aicipirwrc dghsdcsdqs dernctsvtc
      241 ldtkflcpse kkcidksklc dgkqdcddga dekkncsssl crtlscehgc rasleggdcf
      301 cppgmtintk dkrscmdlne csewgycdql ctnspgsyqc lcvrgytldp prhckadnss
      361 amrllftrhn aiysvssdga dlsilanass atgldyhhdr kllfwtdtet kqihsmrlag
      421 dkelrspisv vfswnpvsma vdwvgnklyv cdslgqkidl lemdglrhai vlsrnlsnpq
      481 dialdptvgy mffsdgdhid rahmdgshrt tivtaiykat gltvdyvtkf lvwcdsqldq
      541 ivaidytgsn rniiirgstr vpspnkitlf edkvywtdgt rqgvlqarif nnndtvtivh
      601 rdrqlskepg aiksyhplrq pvvsnpcgln nggcqhmciv trgsggfgll gyrcvchtgf
      661 ellpnrkdct rvstfllysq qkfvrgslmn itqdgftdam vpivsksarf vgldfdarer
      721 fiyysdvild viyrvtidgs gkenvlasqn egveglaldw vshnlyyids rkgtlnvisv
      781 knfqyrrtll knlkrpraiv vhpnkgyvfy sewdrpanis rayldgsnvm vfrgvllgwp
      841 nglsidfqtd rlywcdalld hiqhanldgt dvqtinspri khpfslvihg dslfvtdwrl
      901 dailkmhkit gadekiittv eegsrlygik vysrdnqkvd srhpcqfnng gcqklcfpvp
      961 sngsqgpdlv vscgcpygeq lskdnrtcis dpsaeppvqa cpntwdftce nqrcipktwv
     1021 cdgdddcldn sdemnnctrn tcsprefrcg sgrciplsfk cdsdndcgdf sdetgctnvk
     1081 cdnsefscen grcipqswkc dsendcgdgs degdfckekt cayyqftcpg sghciprswv
     1141 cdgdndcfdq adenncppav csssqfqcsn kkqcihesyr cdgvsdcqdg sdeqgcpsla
     1201 pdqcdleksf qckksrvcip klwycdgnad cedgsdepss cgqvecpqnh fkcnnskcif
     1261 kswicdgsdd cgdnsdedar hacvapkfqc ehgqwlcpgv tdrcvnislv cdgkpdcpng
     1321 adegpgcast pckgnkagct hgchvtpmgp lctcpkgevl ndtttcvdln ecliagtcsq
     1381 hctnskgsft caceqgytle lkhkckatnr sdaflvisnr rsilvanldt sflervpvkv
     1441 envvatasdm atntiywsdm svkkvfrltk ggepqvviss gldlveglav dwvarnlywv
     1501 dsrlktievs tldgknhivl lkenisqprg lsldpregvr lmfwtdwgen prvesvgldg
     1561 tmrktivhtk iywpngltld lpnkriyfad skldyidfcn ydgsgrqqvl ahnhyllhph
     1621 sltifedtly wtdrqlnrvl scqkfrgtnq tvvshlvsqp lgihvnhpvl qppssnpcat
     1681 apcshlclls psrpsqyscr cppgytqdra sasgscvpvd tpflmlmkan qlidisltpd
     1741 dkssgqivpi igiengydfd ydkqegniyw iqladedken gtiytvslkg gnvtkflmdg
     1801 iigapyaiaf dwvgrnlyig nrkssnieiv kvdgekqyrr vlltndgsek gigkpkaivv
     1861 dpeqgklfwl ddggigvprk larvsmdgkn piilvkenlr rlealtidlt gkrvywsqsf
     1921 egiiesvdld gngrktliss taglakpqgl tvfsnklyyl dsvhekiarv dlpdggnqvv
     1981 ledntpglvt lkvygkkpge gdshpcrsnn ggceqlcipv snnqrqcmcs tgykgngdts
     2041 cmpyssflvv seldkargfs ledhgeamqp iagpghnilh ldvhvamnhi ywiefnqger
     2101 ngiyrikpdg tdlkhviadg igsngirgva vdwvagsiyf tnvfphetyi evceldgsnr
     2161 villktttds preiavnpvk rflywidygq ypkiekayld gtnrvpivvt gisnprdlti
     2221 divthdvywv dsredavqkv afsggnrqyv rknlpnpmgv avsgqyvywa drnlrtiyka
     2281 skfqtntstp pepvksdlnm lrdivvfdss nqppasspcs trpcdqlcfs mpesyngtkw
     2341 kcgcasgela adgrkcdmsk vflvfttrre irslfmdphk tsvpfqprgn ltnvvgldfd
     2401 yadkrmyftq irpdgsiswv npdkmdihvv lnktinpegi afdwtakkiy wtdsanrsiy
     2461 amnrdgtqlv miahverpra ivldpcqgym yytdwgrygn tgkiyratma gnnkqaiveq
     2521 nltqpsglai dyddkklywt dalrekiers dldgknretl vsatiypfai tvyenyiywt
     2581 dlqlrgvyra ekhtgagmie mvkrleespr dihvyspdrq kcdknpcslq nggcahschq
     2641 apngtieclc nstfkvaneg rmcvpenvsc ddtkfacgng kciprlwacd gdddcgdntd
     2701 edksfcaqht cgptefrcgn grcifktwkc dhendcgdst deegceyppc pdgeftcanf
     2761 rcipktqlcn gvndckdnkt sdeshancpn nrtcpgnhlk cettnicvep ywlcdgdndc
     2821 gdnsdevall csqrtcppns frcpnhrcip atwhcdgddd cgdkadeped ycksekrtcf
     2881 gdlftcdngn cvpriyicdg dndcldnsde dvrhqcdtre cdaerefkct qnkqwgratc
     2941 ipkrwvcdgd pdcvdgaden ttahncpape pcdpekqyqc knqrcinkdw vcdhdndcgd
     3001 gsdeprnctf rnctseeftc rnakcirksy hcdgeddcgd gsdelekdck keettcsgaq
     3061 frcksgqcip yervcnkqqd csdgsdepah cnvdecakve lnqcehkcvn tltsfycecn
     3121 dgyellkdgk acadinecqv kpavcsqycv nspgsyfckc nqtyydrefd ghtckrkddi
     3181 tpwlifsnry ylrntsldgt lynlvkmnlr nvvaidfdyr egriyfadvg nktiqriytn
     3241 gtgeqvlvrh dvhgleglav dwigrklywl drtskqmyva eldgtsrktl lpkgisdpra
     3301 vavhpgigyl fftdwghhaf igrlgldgtn ftrivlyenk lvwpnaltid yfsdklfwad
     3361 ahldyiefsd ldgknrhtvl qgrtvphvfa ltvfddwlfw tdwnmkgvyr ahkftgeklq
     3421 vlrntshrpy dihvyhplrq lpypnpcgtn nggcshlcli spgassfrca cpdnfvllpd
     3481 ektciancte gqfrcggsdd rcisvfwkcd gekdcrdgsd evgcpafsck kgqfqchnna
     3541 tcvprikicd ghndcgdqsd ekfcgypcge hnfkcqntgr cvpvswqcdg dndctdgsde
     3601 dpaichhqec ntdtqykcnn gkcipklwyc dfdddcgdns depahkcrnr ncttgwqkcp
     3661 svnnyrcipt wlfcdgkddc rdnsdetspd ycpkchetgd fkcrnnrcip lrwrcdfedd
     3721 cgdnsdedst mcaelyrdcs esefqcankk cipsrwrcdh dndcddgsde kdclnyecke
     3781 nqfkcnsghc ilsrlvcdgn kdchdvsdeq ncptrvpggl ycqenmfqcn ntvcirpdfm
     3841 cdgdddcgdf sdesdsickd hkcdvtrkfq cqnnkciplw qlcngnddcg dgsdennhql
     3901 cgkltplpcl atefkctngk cipraevcnh kddcgdlsde rgchegtcda atrggcehnc
     3961 ttlgdsngyi ctcasgykik dddpkrcedi necatfghnc sqicenlmgd ygcrckegye
     4021 mfkshchakg papivlytng pdirmldmkn evqssvitge srvqaldydp rehmlywtds
     4081 yektikrafl pnltdvsagi gfpqnlelkg ltkpnaiavd wvgrtiywmd adlsstrpkg
     4141 rifvslidgr yrktllssnl erptslavdp dmgwmfwsds gsnpkietaw mdgskrriiv
     4201 gdkigyptgl tidyasdhri ywcdsklnti etakqdgsnr vvivsgdlmh pfamdlfeds
     4261 ifwvtrdsge iyrqdkhgrg vkvrvkrnve nptgvkifqp lkynltvpnr cpssgcshlc
     4321 llvpqgyrca cpdgtslhvl nfgscdagfe vpkpqpykcp crnggrcesv rdeqivcrcs
     4381 seyegmhcen fiarrrissl ggasgvaavl vpiivvliim ivaatlfvcv rrrhfkqsgl
     4441 ggstqsvsfr sgtnvefspn fmrtrqaetn aesldadfgl ndvnkatdfs npmydalgna
     4501 dgtagagetg kgglyevagd vfdkkydknc fdssnkapps vgsavlhpsm vlhrssphvp
     4561 lrqtaldptt vdtdkdtqnl veedksec
//
LOCUS       XP_077501217            4587 aa            linear   INV 25-NOV-2025
DEFINITION  low-density lipoprotein receptor-related protein megalin isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077501217
VERSION     XP_077501217.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645091.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..4587
                     /product="low-density lipoprotein receptor-related protein
                     megalin isoform X3"
                     /calculated_mol_wt=512581
     Region          34..66
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(40,48,59..60)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(52,55,59,65..66)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            62..66
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          74..111
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(80,93,104..105)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(97,100,104,110..111)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            107..111
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          120..154
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(125,133,144..145)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(137,140,144,150..151)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            147..151
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          161..196
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(166,175,186..187)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(179,182,186,192..193)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            189..193
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          201..235
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(206,214,225..226)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(218,221,225,231..232)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            228..232
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          240..276
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(245,254,265..266)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(258,261,265,271..272)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            268..272
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          317..355
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(317,320,333)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          <371..>516
                     /region_name="YncE"
                     /note="DNA-binding beta-propeller fold protein YncE
                     [General function prediction only]; COG3391"
                     /db_xref="CDD:442618"
     Region          470..509
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          627..669
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     Region          <712..741
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          786..829
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          814..>939
                     /region_name="YncE"
                     /note="DNA-binding beta-propeller fold protein YncE
                     [General function prediction only]; COG3391"
                     /db_xref="CDD:442618"
     Region          837..872
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          945..988
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     Region          1007..1035
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1007,1015,1026..1027)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1019,1022,1026,1032..1033)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1029..1033
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1042..1076
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1047,1055,1066..1067)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1059,1062,1066,1072..1073)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1069..1073
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1080..1112
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(1086,1094,1105..1106)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1098,1101,1105,1111..1112)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1108..1112
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1121..1156
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1126,1135,1146..1147)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1139,1142,1146,1152..1153)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1149..1153
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1161..1196
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1166,1175,1186..1187)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1179,1182,1186,1192..1193)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1189..1193
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1210..1237
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(1210,1219,1230..1231)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1223,1226,1230,1236..1237)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1233..1237
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1245..1277
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(1251,1259,1270..1271)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1263,1266,1270,1276..1277)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1273..1277
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1283..1323
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(1295,1305,1316..1317)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1309,1312,1316,1322..1323)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1319..1323
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1475..1517
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          1540..1580
                     /region_name="Ldl_recept_b"
                     /note="Low-density lipoprotein receptor repeat class B;
                     pfam00058"
                     /db_xref="CDD:459654"
     Region          1564..1606
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          1765..>1952
                     /region_name="YvrE"
                     /note="Sugar lactone lactonase YvrE [Carbohydrate
                     transport and metabolism]; COG3386"
                     /db_xref="CDD:442613"
     Region          2114..2160
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          2181..2222
                     /region_name="Ldl_recept_b"
                     /note="Low-density lipoprotein receptor repeat class B;
                     pfam00058"
                     /db_xref="CDD:459654"
     Region          2207..2247
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          2402..>2589
                     /region_name="YvrE"
                     /note="Sugar lactone lactonase YvrE [Carbohydrate
                     transport and metabolism]; COG3386"
                     /db_xref="CDD:442613"
     Region          2515..2557
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          2711..2745
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(2716,2724,2735..2736)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2728,2731,2735,2741..2742)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2738..2742
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2750..2785
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(2755,2763,2774..2775)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2767,2770,2774,2782..2783)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            order(2777..2778,2781..2783)
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2793..2826
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(2799,2808,2819..2820)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2812,2815,2819,2825..2826)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2822..2826
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2878..2910
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(2884,2892,2903..2904)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2896,2899,2903,2909..2910)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2906..2910
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2926..2961
                     /region_name="Ldl_recept_a"
                     /note="Low-density lipoprotein receptor domain class A;
                     pfam00057"
                     /db_xref="CDD:395011"
     Site            order(2927,2941,2952..2953)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2945,2948,2952,2958..2959)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2955..2959
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2974..3004
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(2978,2986,2997..2998)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2990,2993,2997,3003..3004)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3000..3004
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3013..3044
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(3018,3026,3037..3038)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3030,3033,3037,3043..3044)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3040..3044
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3055..3087
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(3061,3069,3080..3081)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3073,3076,3080,3086..3087)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3083..3087
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          <3093..3131
                     /region_name="vWFA"
                     /note="Von Willebrand factor type A (vWA) domain was
                     originally found in the blood coagulation protein von
                     Willebrand factor (vWF). Typically, the vWA domain is made
                     up of approximately 200 amino acid residues folded into a
                     classic a/b para-rossmann type of...; cl00057"
                     /db_xref="CDD:469594"
     Region          <3129..3164
                     /region_name="vWFA"
                     /note="Von Willebrand factor type A (vWA) domain was
                     originally found in the blood coagulation protein von
                     Willebrand factor (vWF). Typically, the vWA domain is made
                     up of approximately 200 amino acid residues folded into a
                     classic a/b para-rossmann type of...; cl00057"
                     /db_xref="CDD:469594"
     Region          <3214..3404
                     /region_name="Vgb"
                     /note="Streptogramin lyase [Defense mechanisms]; COG4257"
                     /db_xref="CDD:443399"
     Region          3245..3287
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          3335..3376
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          3447..3484
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     Region          3488..3524
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3493,3503,3514..3515)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3507,3510,3514,3520..3521)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3517..3521
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3529..3564
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3534,3543,3554..3555)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3547,3550,3554,3560..3561)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3557..3561
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3568..3601
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3573,3582,3593..3594)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3586,3589,3593,3599..3600)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3596..3600
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3612..3642
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3616,3624,3635..3636)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3628,3631,3635,3641..3642)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3638..3642
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3652..3686
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(3657,3668,3679..3680)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3672,3675,3679,3685..3686)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3682..3686
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3694..3727
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(3701,3709,3720..3721)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3713,3716,3720,3726..3727)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3723..3727
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3738..3772
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3743,3751,3762..3763)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3755,3758,3762,3768..3769)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3765..3769
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3777..3811
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3782,3790,3801..3802)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3794,3797,3801,3807..3808)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3804..3808
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3867..3896
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3868,3876,3887..3888)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3880,3883,3887,3893..3894)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3890..3894
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3908..3942
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3913,3921,3932..3933)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3925,3928,3932,3938..3939)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3935..3939
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3988..4017
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF domain; pfam07645"
                     /db_xref="CDD:429571"
     Region          4055..4089
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          4109..4151
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          4152..4195
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          4196..4239
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          4314..4337
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     CDS             1..4587
                     /gene="mgl"
                     /coded_by="XM_077645091.1:69..13832"
                     /db_xref="GeneID:144111991"
ORIGIN      
        1 mgfsdnrrvl lgtvcvllcv tsasdsaeve etttcstdnf rcsngrcipl rwvcdyqkdc
       61 eegedehqsc appqcnsnqf scgqyvfnqs ycipkhwrcd qvvdcvdgtd esgdckyrvc
      121 eetdhkcgsg lcvpaskkcd gyydcrdesd eascttngta crlnefrcqh gnkcideskk
      181 cdhwddcgdn sdeegcdfpp cnegqfrctn aicipirwrc dghsdcsdqs dernctsvtc
      241 ldtkflcpse kkcidksklc dgkqdcddga dekkncsssl crtlscehgc rasleggdcf
      301 cppgmtintk dkrscmdlne csewgycdql ctnspgsyqc lcvrgytldp prhckadnss
      361 amrllftrhn aiysvssdga dlsilanass atgldyhhdr kllfwtdtet kqihsmrlag
      421 dkelrspisv vfswnpvsma vdwvgnklyv cdslgqkidl lemdglrhai vlsrnlsnpq
      481 dialdptvgy mffsdgdhid rahmdgshrt tivtaiykat gltvdyvtkf lvwcdsqldq
      541 ivaidytgsn rniiirgstr vpspnkitlf edkvywtdgt rqgvlqarif nnndtvtivh
      601 rdrqlskepg aiksyhplrq pvvsnpcgln nggcqhmciv trgsggfgll gyrcvchtgf
      661 ellpnrkdct rvstfllysq qkfvrgslmn itqdgftdam vpivsksarf vgldfdarer
      721 fiyysdvild viyrvtidgs gkenvlasqn egveglaldw vshnlyyids rkgtlnvisv
      781 knfqyrrtll knlkrpraiv vhpnkgyvfy sewdrpanis rayldgsnvm vfrgvllgwp
      841 nglsidfqtd rlywcdalld hiqhanldgt dvqtinspri khpfslvihg dslfvtdwrl
      901 dailkmhkit gadekiittv eegsrlygik vysrdnqkvd srhpcqfnng gcqklcfpvp
      961 sngsqgpdlv vscgcpygeq lskdnrtcis dpsaeppvqa cpntwdftce nqrcipktwv
     1021 cdgdddcldn sdemnnctrn tcsprefrcg sgrciplsfk cdsdndcgdf sdetgctnvk
     1081 cdnsefscen grcipqswkc dsendcgdgs degdfckekt cayyqftcpg sghciprswv
     1141 cdgdndcfdq adenncppav csssqfqcsn kkqcihesyr cdgvsdcqdg sdeqgcpsla
     1201 pdqcdleksf qckksrvcip klwycdgnad cedgsdepss cgqvecpqnh fkcnnskcif
     1261 kswicdgsdd cgdnsdedar hacvapkfqc ehgqwlcpgv tdrcvnislv cdgkpdcpng
     1321 adegpgcast pckgnkagct hgchvtpmgp lctcpkgevl ndtttcvdln ecliagtcsq
     1381 hctnskgsft caceqgytle lkhkckatnr sdaflvisnr rsilvanldt sflervpvkv
     1441 envvatasdm atntiywsdm svkkvfrltk ggepqvviss gldlveglav dwvarnlywv
     1501 dsrlktievs tldgknhivl lkenisqprg lsldpregvr lmfwtdwgen prvesvgldg
     1561 tmrktivhtk iywpngltld lpnkriyfad skldyidfcn ydgsgrqqvl ahnhyllhph
     1621 sltifedtly wtdrqlnrvl scqkfrgtnq tvvshlvsqp lgihvnhpvl qppssnpcat
     1681 apcshlclls psrpsqyscr cppgytqdra sasgscvpvd tpflmlmkan qlidisltpd
     1741 dkssgqivpi igiengydfd ydkqegniyw iqladedken gtiytvslkg gnvtkflmdg
     1801 iigapyaiaf dwvgrnlyig nrkssnieiv kvdgekqyrr vlltndgsek gigkpkaivv
     1861 dpeqgklfwl ddggigvprk larvsmdgkn piilvkenlr rlealtidlt gkrvywsqsf
     1921 egiiesvdld gngrktliss taglakpqgl tvfsnklyyl dsvhekiarv dlpdggnqvv
     1981 ledntpglvt lkvygkkpge gdshpcrsnn ggceqlcipv snnqrqcmcs tgykgngdts
     2041 cmpyssflvv seldkargfs ledhgeamqp iagpghnilh ldvhvamnhi ywiefnqger
     2101 ngiyrikpdg tdlkhviadg igsngirgva vdwvagniyf tnvfphetyi evceldgsnr
     2161 villktttds preiavnpvk rflywidygq ypkiekayld gtnrvpivvt gisnprdlti
     2221 divthdvywv dsredavqkv afsggnrqyv rknlpnpmgv avsgqyvywa drnlrtiyka
     2281 skfqtntstp pepvksdlnm lrdivvfdss nqppasspcs trpcdqlcfs mpesyngtkw
     2341 kcgcasgela adgrkcdmsk vflvfttrre irslfmdphk tsvpfqprgn ltnvvgldfd
     2401 yadkrmyftq irpdgsiswv npdkmdihvv lnktinpegi afdwtakkiy wtdsanrsiy
     2461 amnrdgtqlv miahverpra ivldpcqgym yytdwgrygn tgkiyratma gnnkqaiveq
     2521 nltqpsglai dyddkklywt dalrekiers dldgknretl vsatiypfai tvyenyiywt
     2581 dlqlrgvyra ekhtgagmie mvkrleespr dihvyspdrq kcdknpcslq nggcahschq
     2641 apngtieclc nstfkvaneg rmcvpenvsc ddtkfacgng kciprlwacd gdddcgdntd
     2701 edksfcaqht cgptefrcgn grcifktwkc dhendcgdst deegceyppc pdgeftcanf
     2761 rcipktqlcn gvndckdnkt sdeshancpn nrtcpgnhlk cettnicvep ywlcdgdndc
     2821 gdnsdevall csqrtcppns frcpnhrcip atwhcdgddd cgdkadeped ycksekrtcf
     2881 gdlftcdngn cvpriyicdg dndcldnsde dvrhqcdtre cdaerefkct qnkqwgratc
     2941 ipkrwvcdgd pdcvdgaden ttahncpape pcdpekqyqc knqrcinkdw vcdhdndcgd
     3001 gsdeprnctf rnctseeftc rnakcirksy hcdgeddcgd gsdelekdck keettcsgaq
     3061 frcksgqcip yervcnkqqd csdgsdepah cnvdecakve lnqcehkcvn tltsfycecn
     3121 dgyellkdgk acadinecqv kpavcsqycv nspgsyfckc nqtyydrefd ghtckrkddi
     3181 tpwlifsnry ylrntsldgt lynlvkmnlr nvvaidfdyr egriyfadvg nktiqriytn
     3241 gtgeqvlvrh dvhgleglav dwigrklywl drtskqmyva eldgtsrktl lpkgisdpra
     3301 vavhpgigyl fftdwghhaf igrlgldgtn ftrivlyenk lvwpnaltid yfsdklfwad
     3361 ahldyiefsd ldgknrhtvl qgrtvphvfa ltvfddwlfw tdwnmkgvyr ahkftgeklq
     3421 vlrntshrpy dihvyhplrq lpypnpcgtn nggcshlcli spgassfrca cpdnfvllpd
     3481 ektciancte gqfrcggsdd rcisvfwkcd gekdcrdgsd evgcpafsck kgqfqchnna
     3541 tcvprikicd ghndcgdqsd ekfcgypcge hnfkcqntgr cvpvswqcdg dndctdgsde
     3601 dpaichhqec ntdtqykcnn gkcipklwyc dfdddcgdns depahkcrnr ncttgwqkcp
     3661 svnnyrcipt wlfcdgkddc rdnsdetspd ycpkchetgd fkcrnnrcip lrwrcdfedd
     3721 cgdnsdedst mselyrdcse sefqcankkc ipsrwrcdhd ndcddgsdek dclnyecken
     3781 qfkcnsghci lsrlvcdgnk dchdvsdeqn cptrvpggly cqenmfqcnn tvcirpdfmc
     3841 dgdddcgdfs desdsickdh kcdvtrkfqc qnnkciplwq lcngnddcgd gsdennhqlc
     3901 gkltplpcla tefkctngkc ipraevcnhk ddcgdlsder gchegtcdaa trggcehnct
     3961 tlgdsngyic tcasgykikd ddpkrcedin ecatfghncs qicenlmgdy gcrckegyem
     4021 fkshchakgp apivlytngp dirmldmkne vqssvitges rvqaldydpr ehmlywtdsy
     4081 ektikraflp nltdvsagig fpqnlelkgl tkpnaiavdw vgrtiywmda dlsstrpkgr
     4141 ifvslidgry rktllssnle rptslavdpd mgwmfwsdsg snpkietawm dgskrriivg
     4201 dkigyptglt idyasdhriy wcdsklntie takqdgsnrv vivsgdlmhp famdlfedsi
     4261 fwvtrdsgei yrqdkhgrgv kvrvkrnven ptgvkifqpl kynltvpnrc pssgcshlcl
     4321 lvpqgyrcac pdgtslhvln fgscdagfev pkpqpykcpc rnggrcesvr deqivcrcss
     4381 eyegmhcenf iarrrisslg gasgvaavlv piivvliimi vaatlfvcvr rrhfkqsglg
     4441 gstqsvsfrs gtnvefspnf mrtrqaetna esldadfgln dvnkatdfsn pmydalgnad
     4501 gtagagetgk gglyevagdv fdkkydkncf dssnkappsv gsavlhpsmv lhrssphvpl
     4561 rqtaldpttv dtdkdtqnlv eedksec
//
LOCUS       XP_077501221            4543 aa            linear   INV 25-NOV-2025
DEFINITION  low-density lipoprotein receptor-related protein megalin isoform X4
            [Amblyomma americanum].
ACCESSION   XP_077501221
VERSION     XP_077501221.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645095.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..4543
                     /product="low-density lipoprotein receptor-related protein
                     megalin isoform X4"
                     /calculated_mol_wt=507642
     Region          34..66
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(40,48,59..60)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(52,55,59,65..66)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            62..66
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          75..109
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(80,88,99..100)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(92,95,99,105..106)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            102..106
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          116..151
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(121,130,141..142)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(134,137,141,147..148)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            144..148
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          156..190
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(161,169,180..181)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(173,176,180,186..187)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            183..187
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          195..231
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(200,209,220..221)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(213,216,220,226..227)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            223..227
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          272..310
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(272,275,288)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          <326..>471
                     /region_name="YncE"
                     /note="DNA-binding beta-propeller fold protein YncE
                     [General function prediction only]; COG3391"
                     /db_xref="CDD:442618"
     Region          425..464
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          582..624
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     Region          <667..696
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          741..784
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          769..>894
                     /region_name="YncE"
                     /note="DNA-binding beta-propeller fold protein YncE
                     [General function prediction only]; COG3391"
                     /db_xref="CDD:442618"
     Region          792..827
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          900..943
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     Region          962..990
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(962,970,981..982)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(974,977,981,987..988)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            984..988
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          997..1031
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1002,1010,1021..1022)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1014,1017,1021,1027..1028)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1024..1028
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1035..1067
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(1041,1049,1060..1061)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1053,1056,1060,1066..1067)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1063..1067
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1076..1111
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1081,1090,1101..1102)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1094,1097,1101,1107..1108)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1104..1108
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1116..1151
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1121,1130,1141..1142)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1134,1137,1141,1147..1148)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1144..1148
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1165..1192
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(1165,1174,1185..1186)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1178,1181,1185,1191..1192)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1188..1192
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1200..1232
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(1206,1214,1225..1226)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1218,1221,1225,1231..1232)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1228..1232
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1238..1278
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(1250,1260,1271..1272)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1264,1267,1271,1277..1278)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1274..1278
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1323..1353
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain; smart00179"
                     /db_xref="CDD:214542"
     Region          1430..1472
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          1495..1535
                     /region_name="Ldl_recept_b"
                     /note="Low-density lipoprotein receptor repeat class B;
                     pfam00058"
                     /db_xref="CDD:459654"
     Region          1519..1561
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          1720..>1907
                     /region_name="YvrE"
                     /note="Sugar lactone lactonase YvrE [Carbohydrate
                     transport and metabolism]; COG3386"
                     /db_xref="CDD:442613"
     Region          2069..2115
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          2136..2177
                     /region_name="Ldl_recept_b"
                     /note="Low-density lipoprotein receptor repeat class B;
                     pfam00058"
                     /db_xref="CDD:459654"
     Region          2162..2202
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          2357..>2544
                     /region_name="YvrE"
                     /note="Sugar lactone lactonase YvrE [Carbohydrate
                     transport and metabolism]; COG3386"
                     /db_xref="CDD:442613"
     Region          2470..2512
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          2666..2700
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(2671,2679,2690..2691)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2683,2686,2690,2696..2697)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2693..2697
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2705..2740
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(2710,2718,2729..2730)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2722,2725,2729,2737..2738)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            order(2732..2733,2736..2738)
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2748..2781
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(2754,2763,2774..2775)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2767,2770,2774,2780..2781)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2777..2781
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2833..2865
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(2839,2847,2858..2859)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2851,2854,2858,2864..2865)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2861..2865
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2881..2916
                     /region_name="Ldl_recept_a"
                     /note="Low-density lipoprotein receptor domain class A;
                     pfam00057"
                     /db_xref="CDD:395011"
     Site            order(2882,2896,2907..2908)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2900,2903,2907,2913..2914)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2910..2914
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2929..2959
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(2933,2941,2952..2953)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2945,2948,2952,2958..2959)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2955..2959
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2968..2999
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(2973,2981,2992..2993)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2985,2988,2992,2998..2999)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2995..2999
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3010..3042
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(3016,3024,3035..3036)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3028,3031,3035,3041..3042)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3038..3042
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          <3048..3086
                     /region_name="vWFA"
                     /note="Von Willebrand factor type A (vWA) domain was
                     originally found in the blood coagulation protein von
                     Willebrand factor (vWF). Typically, the vWA domain is made
                     up of approximately 200 amino acid residues folded into a
                     classic a/b para-rossmann type of...; cl00057"
                     /db_xref="CDD:469594"
     Region          <3084..3119
                     /region_name="vWFA"
                     /note="Von Willebrand factor type A (vWA) domain was
                     originally found in the blood coagulation protein von
                     Willebrand factor (vWF). Typically, the vWA domain is made
                     up of approximately 200 amino acid residues folded into a
                     classic a/b para-rossmann type of...; cl00057"
                     /db_xref="CDD:469594"
     Region          <3169..3359
                     /region_name="Vgb"
                     /note="Streptogramin lyase [Defense mechanisms]; COG4257"
                     /db_xref="CDD:443399"
     Region          3200..3242
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          3290..3331
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          3402..3439
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     Region          3443..3479
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3448,3458,3469..3470)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3462,3465,3469,3475..3476)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3472..3476
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3484..3519
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3489,3498,3509..3510)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3502,3505,3509,3515..3516)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3512..3516
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3523..3556
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3528,3537,3548..3549)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3541,3544,3548,3554..3555)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3551..3555
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3567..3597
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3571,3579,3590..3591)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3583,3586,3590,3596..3597)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3593..3597
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3607..3641
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(3612,3623,3634..3635)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3627,3630,3634,3640..3641)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3637..3641
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3649..3682
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(3656,3664,3675..3676)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3668,3671,3675,3681..3682)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3678..3682
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3694..3728
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3699,3707,3718..3719)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3711,3714,3718,3724..3725)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3721..3725
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3733..3767
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3738,3746,3757..3758)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3750,3753,3757,3763..3764)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3760..3764
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3823..3852
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3824,3832,3843..3844)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3836,3839,3843,3849..3850)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3846..3850
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3864..3898
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3869,3877,3888..3889)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3881,3884,3888,3894..3895)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3891..3895
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3944..3973
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF domain; pfam07645"
                     /db_xref="CDD:429571"
     Region          4011..4045
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          4065..4107
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          4108..4151
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          4152..4195
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          4270..4293
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     CDS             1..4543
                     /gene="mgl"
                     /coded_by="XM_077645095.1:69..13700"
                     /db_xref="GeneID:144111991"
ORIGIN      
        1 mgfsdnrrvl lgtvcvllcv tsasdsaeve etttcstdnf rcsngrcipl rwvcdyqkdc
       61 eegedehqsc eyrvceetdh kcgsglcvpa skkcdgyydc rdesdeasct tngtacrlne
      121 frcqhgnkci deskkcdhwd dcgdnsdeeg cdfppcnegq frctnaicip irwrcdghsd
      181 csdqsdernc tsvtcldtkf lcpsekkcid ksklcdgkqd cddgadekkn cssslcrtls
      241 cehgcrasle ggdcfcppgm tintkdkrsc mdlnecsewg ycdqlctnsp gsyqclcvrg
      301 ytldpprhck adnssamrll ftrhnaiysv ssdgadlsil anassatgld yhhdrkllfw
      361 tdtetkqihs mrlagdkelr spisvvfswn pvsmavdwvg nklyvcdslg qkidllemdg
      421 lrhaivlsrn lsnpqdiald ptvgymffsd gdhidrahmd gshrttivta iykatgltvd
      481 yvtkflvwcd sqldqivaid ytgsnrniii rgstrvpspn kitlfedkvy wtdgtrqgvl
      541 qarifnnndt vtivhrdrql skepgaiksy hplrqpvvsn pcglnnggcq hmcivtrgsg
      601 gfgllgyrcv chtgfellpn rkdctrvstf llysqqkfvr gslmnitqdg ftdamvpivs
      661 ksarfvgldf darerfiyys dvildviyrv tidgsgkenv lasqnegveg laldwvshnl
      721 yyidsrkgtl nvisvknfqy rrtllknlkr praivvhpnk gyvfysewdr panisrayld
      781 gsnvmvfrgv llgwpnglsi dfqtdrlywc dalldhiqha nldgtdvqti nsprikhpfs
      841 lvihgdslfv tdwrldailk mhkitgadek iittveegsr lygikvysrd nqkvdsrhpc
      901 qfnnggcqkl cfpvpsngsq gpdlvvscgc pygeqlskdn rtcisdpsae ppvqacpntw
      961 dftcenqrci pktwvcdgdd dcldnsdemn nctrntcspr efrcgsgrci plsfkcdsdn
     1021 dcgdfsdetg ctnvkcdnse fscengrcip qswkcdsend cgdgsdegdf ckektcayyq
     1081 ftcpgsghci prswvcdgdn dcfdqadenn cppavcsssq fqcsnkkqci hesyrcdgvs
     1141 dcqdgsdeqg cpslapdqcd leksfqckks rvcipklwyc dgnadcedgs depsscgqve
     1201 cpqnhfkcnn skcifkswic dgsddcgdns dedarhacva pkfqcehgqw lcpgvtdrcv
     1261 nislvcdgkp dcpngadegp gcastpckgn kagcthgchv tpmgplctcp kgevlndttt
     1321 cvdlneclia gtcsqhctns kgsftcaceq gytlelkhkc katnrsdafl visnrrsilv
     1381 anldtsfler vpvkvenvva tasdmatnti ywsdmsvkkv frltkggepq vvissgldlv
     1441 eglavdwvar nlywvdsrlk tievstldgk nhivllkeni sqprglsldp regvrlmfwt
     1501 dwgenprves vgldgtmrkt ivhtkiywpn gltldlpnkr iyfadskldy idfcnydgsg
     1561 rqqvlahnhy llhphsltif edtlywtdrq lnrvlscqkf rgtnqtvvsh lvsqplgihv
     1621 nhpvlqppss npcatapcsh lcllspsrps qyscrcppgy tqdrasasgs cvpvdtpflm
     1681 lmkanqlidi sltpddkssg qivpiigien gydfdydkqe gniywiqlad edkengtiyt
     1741 vslkggnvtk flmdgiigap yaiafdwvgr nlyignrkss nieivkvdge kqyrrvlltn
     1801 dgsekgigkp kaivvdpeqg klfwlddggi gvprklarvs mdgknpiilv kenlrrleal
     1861 tidltgkrvy wsqsfegiie svdldgngrk tlisstagla kpqgltvfsn klyyldsvhe
     1921 kiarvdlpdg gnqvvlednt pglvtlkvyg kkpgegdshp crsnnggceq lcipvsnnqr
     1981 qcmcstgykg ngdtscmpys sflvvseldk argfsledhg eamqpiagpg hnilhldvhv
     2041 amnhiywief nqgerngiyr ikpdgtdlkh viadgigsng irgvavdwva gniyftnvfp
     2101 hetyievcel dgsnrvillk tttdspreia vnpvkrflyw idygqypkie kayldgtnrv
     2161 pivvtgisnp rdltidivth dvywvdsred avqkvafsgg nrqyvrknlp npmgvavsgq
     2221 yvywadrnlr tiykaskfqt ntstppepvk sdlnmlrdiv vfdssnqppa sspcstrpcd
     2281 qlcfsmpesy ngtkwkcgca sgelaadgrk cdmskvflvf ttrreirslf mdphktsvpf
     2341 qprgnltnvv gldfdyadkr myftqirpdg siswvnpdkm dihvvlnkti npegiafdwt
     2401 akkiywtdsa nrsiyamnrd gtqlvmiahv erpraivldp cqgymyytdw grygntgkiy
     2461 ratmagnnkq aiveqnltqp sglaidyddk klywtdalre kiersdldgk nretlvsati
     2521 ypfaitvyen yiywtdlqlr gvyraekhtg agmiemvkrl eesprdihvy spdrqkcdkn
     2581 pcslqnggca hschqapngt ieclcnstfk vanegrmcvp envscddtkf acgngkcipr
     2641 lwacdgdddc gdntdedksf caqhtcgpte frcgngrcif ktwkcdhend cgdstdeegc
     2701 eyppcpdgef tcanfrcipk tqlcngvndc kdnktsdesh ancpnnrtcp gnhlkcettn
     2761 icvepywlcd gdndcgdnsd evallcsqrt cppnsfrcpn hrcipatwhc dgdddcgdka
     2821 depedyckse krtcfgdlft cdngncvpri yicdgdndcl dnsdedvrhq cdtrecdaer
     2881 efkctqnkqw gratcipkrw vcdgdpdcvd gadenttahn cpapepcdpe kqyqcknqrc
     2941 inkdwvcdhd ndcgdgsdep rnctfrncts eeftcrnakc irksyhcdge ddcgdgsdel
     3001 ekdckkeett csgaqfrcks gqcipyervc nkqqdcsdgs depahcnvde cakvelnqce
     3061 hkcvntltsf ycecndgyel lkdgkacadi necqvkpavc sqycvnspgs yfckcnqtyy
     3121 drefdghtck rkdditpwli fsnryylrnt sldgtlynlv kmnlrnvvai dfdyregriy
     3181 fadvgnktiq riytngtgeq vlvrhdvhgl eglavdwigr klywldrtsk qmyvaeldgt
     3241 srktllpkgi sdpravavhp gigylfftdw ghhafigrlg ldgtnftriv lyenklvwpn
     3301 altidyfsdk lfwadahldy iefsdldgkn rhtvlqgrtv phvfaltvfd dwlfwtdwnm
     3361 kgvyrahkft geklqvlrnt shrpydihvy hplrqlpypn pcgtnnggcs hlclispgas
     3421 sfrcacpdnf vllpdektci anctegqfrc ggsddrcisv fwkcdgekdc rdgsdevgcp
     3481 afsckkgqfq chnnatcvpr ikicdghndc gdqsdekfcg ypcgehnfkc qntgrcvpvs
     3541 wqcdgdndct dgsdedpaic hhqecntdtq ykcnngkcip klwycdfddd cgdnsdepah
     3601 kcrnrncttg wqkcpsvnny rciptwlfcd gkddcrdnsd etspdycpkc hetgdfkcrn
     3661 nrciplrwrc dfeddcgdns dedstmcael yrdcsesefq cankkcipsr wrcdhdndcd
     3721 dgsdekdcln yeckenqfkc nsghcilsrl vcdgnkdchd vsdeqncptr vpgglycqen
     3781 mfqcnntvci rpdfmcdgdd dcgdfsdesd sickdhkcdv trkfqcqnnk ciplwqlcng
     3841 nddcgdgsde nnhqlcgklt plpclatefk ctngkcipra evcnhkddcg dlsdergche
     3901 gtcdaatrgg cehncttlgd sngyictcas gykikdddpk rcedinecat fghncsqice
     3961 nlmgdygcrc kegyemfksh chakgpapiv lytngpdirm ldmknevqss vitgesrvqa
     4021 ldydprehml ywtdsyekti kraflpnltd vsagigfpqn lelkgltkpn aiavdwvgrt
     4081 iywmdadlss trpkgrifvs lidgryrktl lssnlerpts lavdpdmgwm fwsdsgsnpk
     4141 ietawmdgsk rriivgdkig yptgltidya sdhriywcds klntietakq dgsnrvvivs
     4201 gdlmhpfamd lfedsifwvt rdsgeiyrqd khgrgvkvrv krnvenptgv kifqplkynl
     4261 tvpnrcpssg cshlcllvpq gyrcacpdgt slhvlnfgsc dagfevpkpq pykcpcrngg
     4321 rcesvrdeqi vcrcsseyeg mhcenfiarr risslggasg vaavlvpiiv vliimivaat
     4381 lfvcvrrrhf kqsglggstq svsfrsgtnv efspnfmrtr qaetnaesld adfglndvnk
     4441 atdfsnpmyd algnadgtag agetgkggly evagdvfdkk ydkncfdssn kappsvgsav
     4501 lhpsmvlhrs sphvplrqta ldpttvdtdk dtqnlveedk sec
//
LOCUS       XP_077501228             376 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112022 [Amblyomma americanum].
ACCESSION   XP_077501228
VERSION     XP_077501228.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645102.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..376
                     /product="uncharacterized protein LOC144112022"
                     /calculated_mol_wt=42238
     CDS             1..376
                     /gene="LOC144112022"
                     /coded_by="XM_077645102.1:1303..2433"
                     /db_xref="GeneID:144112022"
ORIGIN      
        1 mkilqvsmdg pnvnlkflrs lkeelreadn shqildigsc glhlvngafk tghsathwdl
       61 vaflrgsynl fkcasarrad ytrytgsvvf plkfcpvrwl enrkvisral dipnllkyve
      121 cskeakkspa sasfstveka vgdrmlpvkl afmlsiaeel epflaefqtd kpmlpfvapa
      181 ldrllrsllg riirkeilqa tntsskllri dlenpdnmvs aaafdigfaa knefrkipkp
      241 sqltvlsfkk dciffvkrcs qkvmersplq ykltrgascl dpasalspea gakklcmale
      301 vltehqwmtg leaerthrss vkvcslssvq valrsfdrke qrldalwfef csrdhkelfl
      361 fvklilcmsh gnaave
//
LOCUS       XP_077501236            1320 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: vacuolar protein sorting 8 [Amblyomma
            americanum].
ACCESSION   XP_077501236
VERSION     XP_077501236.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645110.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1320
                     /product="LOW QUALITY PROTEIN: vacuolar protein sorting 8"
                     /calculated_mol_wt=146165
     Region          <134..279
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          176..213
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          222..264
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          273..305
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          578..759
                     /region_name="Vps8"
                     /note="Golgi CORVET complex core vacuolar protein 8;
                     pfam12816"
                     /db_xref="CDD:463718"
     Region          1225..1267
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(1225,1228,1243,1245,1248,1251,1263,1266)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     CDS             1..1320
                     /gene="Vps8"
                     /coded_by="XM_077645110.1:122..4084"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:144112029"
ORIGIN      
        1 mdefdtvaad rrtlnassas entvlafnie elddkefelp pvenpptles ilneadggsl
       61 sdeevpnqag affheacdsv svssadsrnv aaekidrrsg trghpdsilr hvilrgvssq
      121 lrsaaervds gkptamavss viavgtfhgl vlvfdpeqal kwclgstqlg eeygsvsalg
      181 fnndcsrllc gyakgqltmw dlsngrllrt itdihppqma ilhakftddp tlaicsdsgg
      241 svyelnfrkv lntrtcesvc ifsgsrgevc aveplrvspe varhpsqeva llalatvtkv
      301 ivvsmrpvlr vrfthslkad pdtlpllswq fvviqvsasa riidpvlafg rqntifffqv
      361 nyssldkilf vplqkvqvqy tvqnftwlns rtlaaldtse nlhvldvrsq eelevldvsd
      421 vqmvygtshf kghatggnvs kamvaaaera cyhsvagfgp qvlllglasv hvlslrpwle
      481 rlerlwqrql yaealalahs fyrdeakavc glvgrkarrq qqvaahtrrl iegglapera
      541 rpavplcvri alelrehcpd lldkvyglls adlglkalfl eslpvhvidg elsslspava
      601 kdlvehlast gqmellehtl vhldvtcldl hqvmtlcwkh clydgiiyvh nramgdyttp
      661 iqelmailrq alesgrqlsd shvllgnkll vyiscclagr gyphgelrad lapivkkdvf
      721 qcitslrgtn iqdhatfpyl rallhfstre flnvlalafe epefateagl aqkqrlvdil
      781 lqimvqsegf sptqvgalft flarqlarpd nklivdrllf eqvlehlthp gdetrreerq
      841 qallellqag glasidqehl lnlaeqahfy rvcehlyekq rlynkvflcy ledrsrklqs
      901 fqyatsifgs pdvdeqekaa legqlldsle alvevdnkaa sqlmlrhlah rvddimhrle
      961 gqpkvlyhfl qgvwksreas slaptqgaad slatavqery ielmcqfepn vvcaflstse
     1021 gyrieetlai crrhnvldat ayllekagdv qgasaillen lsqrlqlcaq eaegasqreq
     1081 llqaawsqlq aviqlcqrsc prlsaaerea lwfpvletvm apqrqlrstl gadkdflslt
     1141 hhllgsmmgf valpqilhkl mqdpayssgk fgevrqfimk mlgthnyeka llhttsrlls
     1201 sdvhlhlcql kaaanrayvs htatcatcnk gfsepgdvvm frcrhcyhga clealgpger
     1261 accqlctsrp pasgkgpsqa vsartpqnmp aalhklpmql kdnlqlrlap arlpdikmda
//
LOCUS       XP_077501243             445 aa            linear   INV 25-NOV-2025
DEFINITION  BEN domain-containing protein 5-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501243
VERSION     XP_077501243.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645117.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..445
                     /product="BEN domain-containing protein 5-like isoform X1"
                     /calculated_mol_wt=49403
     CDS             1..445
                     /gene="LOC144112038"
                     /coded_by="XM_077645117.1:94..1431"
                     /db_xref="GeneID:144112038"
ORIGIN      
        1 mfalvrfved dkdkryvisv adienfepkn eldfdntkty daywqdeded nsgiyvvqil
       61 klaatkeeme remkvkrvhv ppirmsevdn vtecgvpaaa rkkslkqqek akqqnqitsk
      121 kqqyedvlkr hvshslqkna aipghssigr kaskkrkpqi lddstesede svvssrelkn
      181 avkeakywkq ryrleqehta slkreldflk rkvelqlssf qqtldevakr qqdlcadalp
      241 vvapsilpng aveasrrdpt aargaateqa aaaekdvggt asvvptpagp seeapasscf
      301 tadaprsael easvlddaqd ftltsgglfh ltqgvyikpd qaeklfknkk psivvrdsaq
      361 llwgietlsk rsvsgrlapt ksgscevpae qltpaklevv ygclrhwgkv hgvdtsvaec
      421 svprilseki qdvkrrlrll pkdnv
//
LOCUS       XP_077501247             441 aa            linear   INV 25-NOV-2025
DEFINITION  BEN domain-containing protein 5-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501247
VERSION     XP_077501247.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645121.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..441
                     /product="BEN domain-containing protein 5-like isoform X2"
                     /calculated_mol_wt=49001
     CDS             1..441
                     /gene="LOC144112038"
                     /coded_by="XM_077645121.1:94..1419"
                     /db_xref="GeneID:144112038"
ORIGIN      
        1 mfalvrfved dkdkryvisv adienfepkn eldfdntkty daywqdeded nsgiyvvqil
       61 klaatkeeme remkvkrvhv ppirmsevdn vtecgvpaaa rkkslkqqek akqqnqitsk
      121 kqqyedvlkr hvshslqkna aipghssigr kaskkrkpqi lddstesede svvssrelkn
      181 avkeakywkq ryrleqehta slkreldflk rkvelqlssf qqtldevakr qqdalpvvap
      241 silpngavea srrdptaarg aateqaaaae kdvggtasvv ptpagpseea passcftada
      301 prsaeleasv lddaqdftlt sgglfhltqg vyikpdqaek lfknkkpsiv vrdsaqllwg
      361 ietlskrsvs grlaptksgs cevpaeqltp aklevvygcl rhwgkvhgvd tsvaecsvpr
      421 ilsekiqdvk rrlrllpkdn v
//
LOCUS       XP_077501254             428 aa            linear   INV 25-NOV-2025
DEFINITION  BEN domain-containing protein 5-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501254
VERSION     XP_077501254.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645128.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..428
                     /product="BEN domain-containing protein 5-like isoform X3"
                     /calculated_mol_wt=47476
     CDS             1..428
                     /gene="LOC144112038"
                     /coded_by="XM_077645128.1:94..1380"
                     /db_xref="GeneID:144112038"
ORIGIN      
        1 mfalvrfved dkdkryvisv adienfepkn eldfdntkty daywqdeded nsgiyvvqil
       61 klaatkeeme remkvkrvhv ppirmsevdn vtecgvpaaa rkkslkqqek akqqnqitsk
      121 kqqyedvlkr hvshslqkna aipghssigr kaskkrkpqi lddstesede svvssrelkn
      181 avkeakywkq ryrleqehta slkreldflk rkvelqlssy alpvvapsil pngaveasrr
      241 dptaargaat eqaaaaekdv ggtasvvptp agpseeapas scftadaprs aeleasvldd
      301 aqdftltsgg lfhltqgvyi kpdqaeklfk nkkpsivvrd saqllwgiet lskrsvsgrl
      361 aptksgscev paeqltpakl evvygclrhw gkvhgvdtsv aecsvprils ekiqdvkrrl
      421 rllpkdnv
//
LOCUS       XP_077501263             401 aa            linear   INV 25-NOV-2025
DEFINITION  BEN domain-containing protein 5-like isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077501263
VERSION     XP_077501263.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645137.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..401
                     /product="BEN domain-containing protein 5-like isoform X4"
                     /calculated_mol_wt=44395
     CDS             1..401
                     /gene="LOC144112038"
                     /coded_by="XM_077645137.1:94..1299"
                     /db_xref="GeneID:144112038"
ORIGIN      
        1 mfalvrfved dkdkryvisv adienfepkn eldfdntkty daywqdeded nsgiyvvqil
       61 klaatkeeme remkvkrvhv ppirmsevdn vtecgvpaaa rkkslkqask krkpqildds
      121 tesedesvvs srelknavke akywkqryrl eqehtaslkr eldflkrkve lqlssfqqtl
      181 devakrqqdl cadalpvvap silpngavea srrdptaarg aateqaaaae kdvggtasvv
      241 ptpagpseea passcftada prsaeleasv lddaqdftlt sgglfhltqg vyikpdqaek
      301 lfknkkpsiv vrdsaqllwg ietlskrsvs grlaptksgs cevpaeqltp aklevvygcl
      361 rhwgkvhgvd tsvaecsvpr ilsekiqdvk rrlrllpkdn v
//
LOCUS       XP_077501272             693 aa            linear   INV 25-NOV-2025
DEFINITION  nitric oxide-associated protein 1 [Amblyomma americanum].
ACCESSION   XP_077501272
VERSION     XP_077501272.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645146.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..693
                     /product="nitric oxide-associated protein 1"
                     /calculated_mol_wt=76989
     Region          198..665
                     /region_name="GTPase_YqeH"
                     /note="ribosome biogenesis GTPase YqeH; TIGR03597"
                     /db_xref="CDD:213834"
     Region          234..419
                     /region_name="YqeH"
                     /note="Circularly permuted YqeH GTPase; cd01855"
                     /db_xref="CDD:206748"
     Site            order(302..303,305..306,342..344,372..378)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206748"
     Site            302..305
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206748"
     Site            342..344
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206748"
     Site            370..377
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206748"
     Site            406..413
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206748"
     Site            408
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206748"
     CDS             1..693
                     /gene="LOC144112066"
                     /coded_by="XM_077645146.1:302..2383"
                     /db_xref="GeneID:144112066"
ORIGIN      
        1 mrlcanchis ryllvsraal sirlrslssp ckrsfssdad dvpisymglg fnkvrlarqk
       61 lrrelrkeer gntlpayfaq lvksdaeqle klypqlaniv kkeltdaape trgepekkyq
      121 fpfqqftass ckpcathelt vnqssherne svskyldslq samqkldise dlagirsdvw
      181 ranygsadat vppsqvpcgg cgaflhcsdt aipgflpaev faglnddqlr qrlcqrcifl
      241 qhyntalnvk vdqdayksvl aevsrrkalv lllldltdmp csiwpdlpel lgpqqpvlav
      301 ankvdlvpcd apgylsrike cvtktlkgag lshnlrgvcl isaktgygve dlitclhdtw
      361 rmkgdiylvg ctnvgkstlf ntllqsdlcq eqatdlvhra ttsvwpgttl nllkfpvlrp
      421 rgwelhlrsr rlardnwalh nevhhrrkly sstlrpeyat ligrigmtfk kggleadlgv
      481 rdrpfkldep klkgthfcyd tpgavykdqi ldlltleell lllprkmitp rtislrcnqs
      541 llltglarld lldvprsqsv lvtvfasell pihvldtpdv drfraahlgt emlgvpcgta
      601 erlaqlpple srdftlegqg lerscadlvf ssvgwlsltf clgervsvrt wspsgrglhc
      661 rvpallpaav qlrgprirgt pafanrqcsa lla
//
LOCUS       XP_077501279             496 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112075 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501279
VERSION     XP_077501279.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645153.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..496
                     /product="uncharacterized protein LOC144112075 isoform X1"
                     /calculated_mol_wt=54603
     Region          <301..>354
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     CDS             1..496
                     /gene="LOC144112075"
                     /coded_by="XM_077645153.1:3761..5251"
                     /db_xref="GeneID:144112075"
ORIGIN      
        1 mearaqnnpt asnittgpiq gqdktqemev vpmttcydar tgtgpsenge eermhqengs
       61 dltqdeetnw eiyfsrksnk grgrandans katthpigkn kmapahtaag gmtqsdsnks
      121 tktegrrghq gqnlpplprs dakiiirprg glkiqdmkpy qatqaivqac ggkikeedfl
      181 vrlrpgsnii iastsnddsa neirkirelp iqgtsyqvqa ymampsdvtr vvihglpsei
      241 spkelesrlr irsqgteila armlgkseta litldgpdpk wiifsageyk typyrptrqc
      301 cfvcgmqghc sdvcptpgar tcrrcgatnp eenhecqpkc micgedhltg trdckrrlkt
      361 aaelrgrqgk pgqqrrshsr grgrrprwfg penesnsrsr srvsqaesrs rsrsrgrshs
      421 rgprdesfpp lgtqgtkqaa skqqqqksgv kglqissaea gakfkapsrq prmegspsap
      481 sdciifrnrp qtacfd
//
LOCUS       XP_077501284             452 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112075 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501284
VERSION     XP_077501284.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645158.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..452
                     /product="uncharacterized protein LOC144112075 isoform X2"
                     /calculated_mol_wt=49884
     Region          <301..>354
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     CDS             1..452
                     /gene="LOC144112075"
                     /coded_by="XM_077645158.1:3761..5119"
                     /db_xref="GeneID:144112075"
ORIGIN      
        1 mearaqnnpt asnittgpiq gqdktqemev vpmttcydar tgtgpsenge eermhqengs
       61 dltqdeetnw eiyfsrksnk grgrandans katthpigkn kmapahtaag gmtqsdsnks
      121 tktegrrghq gqnlpplprs dakiiirprg glkiqdmkpy qatqaivqac ggkikeedfl
      181 vrlrpgsnii iastsnddsa neirkirelp iqgtsyqvqa ymampsdvtr vvihglpsei
      241 spkelesrlr irsqgteila armlgkseta litldgpdpk wiifsageyk typyrptrqc
      301 cfvcgmqghc sdvcptpgar tcrrcgatnp eenhecqpkc micgedhltg trdckrrlkt
      361 aaelrgrqgk pgqqrrshsr grgrrprwfg penesnsrsr srvsqaesrs rsrsrgrshs
      421 rgprdesfpp lgtqgtkqaa skqqqqksgv ks
//
LOCUS       XP_077501300             372 aa            linear   INV 25-NOV-2025
DEFINITION  coiled-coil domain-containing protein 125-like [Amblyomma
            americanum].
ACCESSION   XP_077501300
VERSION     XP_077501300.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645174.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..372
                     /product="coiled-coil domain-containing protein 125-like"
                     /calculated_mol_wt=41219
     Region          <127..>202
                     /region_name="BAR"
                     /note="The Bin/Amphiphysin/Rvs (BAR) domain, a
                     dimerization module that binds membranes and detects
                     membrane curvature; cl12013"
                     /db_xref="CDD:472257"
     CDS             1..372
                     /gene="LOC144112093"
                     /coded_by="XM_077645174.1:53..1171"
                     /db_xref="GeneID:144112093"
ORIGIN      
        1 mrtyqsrqlp aaprvvggta asgamedafe gdlglaerra saslgdltkl pewspasekl
       61 cassaahvca eetsshsaat grcldasgqs gdevvsslqs eldicrrlln akylaahill
      121 kqlvrvseek eegtrrshrq caellkelns lqyelqcmee hlrrsqqtwl dklnslmeen
      181 gdqqrklreq aealrrltyt aqvlrrerde llaltdvker dvylqssaek ldsesssiev
      241 avlaacscrg psqepcrcah aaahkqrqla eltrrakrae aqaqdnakta diyreafevq
      301 laknraltrs lvelqsrrgi qlarmpypad arrliddlle nlcdknealv hqqvmvklla
      361 lqlggrgada tt
//
LOCUS       XP_077501303             258 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112122 [Amblyomma americanum].
ACCESSION   XP_077501303
VERSION     XP_077501303.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645177.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..258
                     /product="uncharacterized protein LOC144112122"
                     /calculated_mol_wt=27781
     CDS             1..258
                     /gene="LOC144112122"
                     /coded_by="XM_077645177.1:466..1242"
                     /db_xref="GeneID:144112122"
ORIGIN      
        1 mpaqpvpghq qtsflpvpve eieartpwrc dsplfghada aletdypisd fralgwsfam
       61 ggpptpapqg tpiwvpygtl idsaanvlmp aqpvpghqqt sflpvpveei eartpwrcds
      121 plfghadaal etdypisdfr algwsfamgg pptpapqgtp iwvpygtlid saanvlmpaq
      181 pvpghqqtsf lpvpveeiea rtpwrcdspl fghadaalet dypisdfral gwsfamgepp
      241 tpapqgtliw vpygtrid
//
LOCUS       XP_077501304             131 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112208 [Amblyomma americanum].
ACCESSION   XP_077501304
VERSION     XP_077501304.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645178.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..131
                     /product="uncharacterized protein LOC144112208"
                     /calculated_mol_wt=14877
     CDS             1..131
                     /gene="LOC144112208"
                     /coded_by="XM_077645178.1:254..649"
                     /db_xref="GeneID:144112208"
ORIGIN      
        1 mvayegtdpe esveeenwdl diapgnsgyi psghlyfqrv qrqsrrtrrr mykeqaedyk
       61 rresrfpgpg lvvcdgraad prtarnahlg pvrntdrlgh qrpdagpact rtpvrlnagp
      121 cdanartwlw w
//
LOCUS       XP_077501305             365 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112224 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501305
VERSION     XP_077501305.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645179.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..365
                     /product="uncharacterized protein LOC144112224 isoform X1"
                     /calculated_mol_wt=38617
     CDS             1..365
                     /gene="LOC144112224"
                     /coded_by="XM_077645179.1:363..1460"
                     /db_xref="GeneID:144112224"
ORIGIN      
        1 mpaqpvpghq qtsflpvpve eiearipwrc dsplfgrada aletdypisd fralgwlfam
       61 ggpptlalqg tliwvpygtr idsatnvlmp aqpvpghqfv vmqvpamrmn grgsggegmv
      121 ppyavpapga fpqghygeqi hyppaaagqs wrrpsvdiay iqppttsyqa plfsipnapp
      181 pfentgmapl eeqlapeeqa aaqqsegagv yaeggalcda plesfaimae qlrehtvsks
      241 gvstpvgppl radswgwqsa vlpepasndk hvppppassd gscpassedp ggsggsqvcr
      301 rryggcipag rmdqggacrp gqllrpckga qrsprclrdr itnglrsstw phdglfpaqp
      361 mllli
//
LOCUS       XP_077501306             365 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112224 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501306
VERSION     XP_077501306.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645180.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..365
                     /product="uncharacterized protein LOC144112224 isoform X1"
                     /calculated_mol_wt=38617
     CDS             1..365
                     /gene="LOC144112224"
                     /coded_by="XM_077645180.1:345..1442"
                     /db_xref="GeneID:144112224"
ORIGIN      
        1 mpaqpvpghq qtsflpvpve eiearipwrc dsplfgrada aletdypisd fralgwlfam
       61 ggpptlalqg tliwvpygtr idsatnvlmp aqpvpghqfv vmqvpamrmn grgsggegmv
      121 ppyavpapga fpqghygeqi hyppaaagqs wrrpsvdiay iqppttsyqa plfsipnapp
      181 pfentgmapl eeqlapeeqa aaqqsegagv yaeggalcda plesfaimae qlrehtvsks
      241 gvstpvgppl radswgwqsa vlpepasndk hvppppassd gscpassedp ggsggsqvcr
      301 rryggcipag rmdqggacrp gqllrpckga qrsprclrdr itnglrsstw phdglfpaqp
      361 mllli
//
LOCUS       XP_077501307             306 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112224 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501307
VERSION     XP_077501307.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645181.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..306
                     /product="uncharacterized protein LOC144112224 isoform X2"
                     /calculated_mol_wt=32023
     CDS             1..306
                     /gene="LOC144112224"
                     /coded_by="XM_077645181.1:625..1545"
                     /db_xref="GeneID:144112224"
ORIGIN      
        1 mggpptlalq gtliwvpygt ridsatnvlm paqpvpghqf vvmqvpamrm ngrgsggegm
       61 vppyavpapg afpqghygeq ihyppaaagq swrrpsvdia yiqppttsyq aplfsipnap
      121 ppfentgmap leeqlapeeq aaaqqsegag vyaeggalcd aplesfaima eqlrehtvsk
      181 sgvstpvgpp lradswgwqs avlpepasnd khvppppass dgscpassed pggsggsqvc
      241 rrryggcipa grmdqggacr pgqllrpckg aqrsprclrd ritnglrsst wphdglfpaq
      301 pmllli
//
LOCUS       XP_077501308             306 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112224 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501308
VERSION     XP_077501308.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645182.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..306
                     /product="uncharacterized protein LOC144112224 isoform X2"
                     /calculated_mol_wt=32023
     CDS             1..306
                     /gene="LOC144112224"
                     /coded_by="XM_077645182.1:622..1542"
                     /db_xref="GeneID:144112224"
ORIGIN      
        1 mggpptlalq gtliwvpygt ridsatnvlm paqpvpghqf vvmqvpamrm ngrgsggegm
       61 vppyavpapg afpqghygeq ihyppaaagq swrrpsvdia yiqppttsyq aplfsipnap
      121 ppfentgmap leeqlapeeq aaaqqsegag vyaeggalcd aplesfaima eqlrehtvsk
      181 sgvstpvgpp lradswgwqs avlpepasnd khvppppass dgscpassed pggsggsqvc
      241 rrryggcipa grmdqggacr pgqllrpckg aqrsprclrd ritnglrsst wphdglfpaq
      301 pmllli
//
LOCUS       XP_077501309             306 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112224 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501309
VERSION     XP_077501309.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645183.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..306
                     /product="uncharacterized protein LOC144112224 isoform X2"
                     /calculated_mol_wt=32023
     CDS             1..306
                     /gene="LOC144112224"
                     /coded_by="XM_077645183.1:728..1648"
                     /db_xref="GeneID:144112224"
ORIGIN      
        1 mggpptlalq gtliwvpygt ridsatnvlm paqpvpghqf vvmqvpamrm ngrgsggegm
       61 vppyavpapg afpqghygeq ihyppaaagq swrrpsvdia yiqppttsyq aplfsipnap
      121 ppfentgmap leeqlapeeq aaaqqsegag vyaeggalcd aplesfaima eqlrehtvsk
      181 sgvstpvgpp lradswgwqs avlpepasnd khvppppass dgscpassed pggsggsqvc
      241 rrryggcipa grmdqggacr pgqllrpckg aqrsprclrd ritnglrsst wphdglfpaq
      301 pmllli
//
LOCUS       XP_077501310             306 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112224 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501310
VERSION     XP_077501310.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645184.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..306
                     /product="uncharacterized protein LOC144112224 isoform X2"
                     /calculated_mol_wt=32023
     CDS             1..306
                     /gene="LOC144112224"
                     /coded_by="XM_077645184.1:725..1645"
                     /db_xref="GeneID:144112224"
ORIGIN      
        1 mggpptlalq gtliwvpygt ridsatnvlm paqpvpghqf vvmqvpamrm ngrgsggegm
       61 vppyavpapg afpqghygeq ihyppaaagq swrrpsvdia yiqppttsyq aplfsipnap
      121 ppfentgmap leeqlapeeq aaaqqsegag vyaeggalcd aplesfaima eqlrehtvsk
      181 sgvstpvgpp lradswgwqs avlpepasnd khvppppass dgscpassed pggsggsqvc
      241 rrryggcipa grmdqggacr pgqllrpckg aqrsprclrd ritnglrsst wphdglfpaq
      301 pmllli
//
LOCUS       XP_077501311             306 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112224 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501311
VERSION     XP_077501311.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645185.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..306
                     /product="uncharacterized protein LOC144112224 isoform X2"
                     /calculated_mol_wt=32023
     CDS             1..306
                     /gene="LOC144112224"
                     /coded_by="XM_077645185.1:1081..2001"
                     /db_xref="GeneID:144112224"
ORIGIN      
        1 mggpptlalq gtliwvpygt ridsatnvlm paqpvpghqf vvmqvpamrm ngrgsggegm
       61 vppyavpapg afpqghygeq ihyppaaagq swrrpsvdia yiqppttsyq aplfsipnap
      121 ppfentgmap leeqlapeeq aaaqqsegag vyaeggalcd aplesfaima eqlrehtvsk
      181 sgvstpvgpp lradswgwqs avlpepasnd khvppppass dgscpassed pggsggsqvc
      241 rrryggcipa grmdqggacr pgqllrpckg aqrsprclrd ritnglrsst wphdglfpaq
      301 pmllli
//
LOCUS       XP_077501312             306 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112224 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501312
VERSION     XP_077501312.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645186.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..306
                     /product="uncharacterized protein LOC144112224 isoform X2"
                     /calculated_mol_wt=32023
     CDS             1..306
                     /gene="LOC144112224"
                     /coded_by="XM_077645186.1:1078..1998"
                     /db_xref="GeneID:144112224"
ORIGIN      
        1 mggpptlalq gtliwvpygt ridsatnvlm paqpvpghqf vvmqvpamrm ngrgsggegm
       61 vppyavpapg afpqghygeq ihyppaaagq swrrpsvdia yiqppttsyq aplfsipnap
      121 ppfentgmap leeqlapeeq aaaqqsegag vyaeggalcd aplesfaima eqlrehtvsk
      181 sgvstpvgpp lradswgwqs avlpepasnd khvppppass dgscpassed pggsggsqvc
      241 rrryggcipa grmdqggacr pgqllrpckg aqrsprclrd ritnglrsst wphdglfpaq
      301 pmllli
//
LOCUS       XP_077501313             306 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112224 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501313
VERSION     XP_077501313.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645187.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..306
                     /product="uncharacterized protein LOC144112224 isoform X2"
                     /calculated_mol_wt=32023
     CDS             1..306
                     /gene="LOC144112224"
                     /coded_by="XM_077645187.1:1063..1983"
                     /db_xref="GeneID:144112224"
ORIGIN      
        1 mggpptlalq gtliwvpygt ridsatnvlm paqpvpghqf vvmqvpamrm ngrgsggegm
       61 vppyavpapg afpqghygeq ihyppaaagq swrrpsvdia yiqppttsyq aplfsipnap
      121 ppfentgmap leeqlapeeq aaaqqsegag vyaeggalcd aplesfaima eqlrehtvsk
      181 sgvstpvgpp lradswgwqs avlpepasnd khvppppass dgscpassed pggsggsqvc
      241 rrryggcipa grmdqggacr pgqllrpckg aqrsprclrd ritnglrsst wphdglfpaq
      301 pmllli
//
LOCUS       XP_077501315             186 aa            linear   INV 25-NOV-2025
DEFINITION  TATA-box-binding protein-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501315
VERSION     XP_077501315.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645189.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..186
                     /product="TATA-box-binding protein-like isoform X1"
                     /calculated_mol_wt=20624
     Region          15..180
                     /region_name="TBP_eukaryotes"
                     /note="eukaryotic TATA box binding protein (TBP): Present
                     in archaea and eukaryotes, TBPs are transcription factors
                     that recognize promoters and initiate transcription. TBP
                     has been shown to be an essential component of three
                     different transcription initiation...; cd04516"
                     /db_xref="CDD:239952"
     Site            order(15,17,44..45,49,51,58,60,64,66,68,70,100,105,107,
                     136..137,142,153,155,157,161)
                     /site_type="other"
                     /note="DNA interaction surface [nucleotide binding]"
                     /db_xref="CDD:239952"
     Site            order(29,36..38,40,45,51,53)
                     /site_type="other"
                     /note="TFIIA interaction surface [polypeptide binding]"
                     /db_xref="CDD:239952"
     Site            order(44,141..142,147,178..179)
                     /site_type="other"
                     /note="NC2 interaction surface [polypeptide binding]"
                     /db_xref="CDD:239952"
     Site            order(117..118,130..134,137,142)
                     /site_type="other"
                     /note="TFIIB interaction surface [polypeptide binding]"
                     /db_xref="CDD:239952"
     CDS             1..186
                     /gene="LOC144112322"
                     /coded_by="XM_077645189.1:1933..2493"
                     /db_xref="GeneID:144112322"
ORIGIN      
        1 mhklsvipat tfhrntmasl slgcrldlrk ialhapntey dpkrtpalvm rigepramal
       61 vfgsgkilcs gakdemkakl asrkfariiq klgfdahfln fkivnivasc dvgflirleg
      121 lilarrglas yepelspglt yrpehprvtl llfpsgkivf tgskteeein epfkdiysrl
      181 qtcrig
//
LOCUS       XP_077501317             173 aa            linear   INV 25-NOV-2025
DEFINITION  TATA-box-binding protein-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077501317
VERSION     XP_077501317.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645191.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..173
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..173
                     /product="TATA-box-binding protein-like isoform X2"
                     /calculated_mol_wt=19136
     Region          2..167
                     /region_name="TBP_eukaryotes"
                     /note="eukaryotic TATA box binding protein (TBP): Present
                     in archaea and eukaryotes, TBPs are transcription factors
                     that recognize promoters and initiate transcription. TBP
                     has been shown to be an essential component of three
                     different transcription initiation...; cd04516"
                     /db_xref="CDD:239952"
     Site            order(2,4,31..32,36,38,45,47,51,53,55,57,87,92,94,
                     123..124,129,140,142,144,148)
                     /site_type="other"
                     /note="DNA interaction surface [nucleotide binding]"
                     /db_xref="CDD:239952"
     Site            order(16,23..25,27,32,38,40)
                     /site_type="other"
                     /note="TFIIA interaction surface [polypeptide binding]"
                     /db_xref="CDD:239952"
     Site            order(31,128..129,134,165..166)
                     /site_type="other"
                     /note="NC2 interaction surface [polypeptide binding]"
                     /db_xref="CDD:239952"
     Site            order(104..105,117..121,124,129)
                     /site_type="other"
                     /note="TFIIB interaction surface [polypeptide binding]"
                     /db_xref="CDD:239952"
     CDS             1..173
                     /gene="LOC144112322"
                     /coded_by="XM_077645191.1:99..620"
                     /db_xref="GeneID:144112322"
ORIGIN      
        1 mntmaslslg crldlrkial hapnteydpk rtpalvmrig epramalvfg sgkilcsgak
       61 demkaklasr kfariiqklg fdahflnfki vnivascdvg flirleglil arrglasyep
      121 elspgltyrp ehprvtlllf psgkivftgs kteeeinepf kdiysrlqtc rig
//
LOCUS       XP_077501319             196 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112336 [Amblyomma americanum].
ACCESSION   XP_077501319
VERSION     XP_077501319.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645193.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..196
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..196
                     /product="uncharacterized protein LOC144112336"
                     /calculated_mol_wt=22165
     Region          <120..163
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     CDS             1..196
                     /gene="LOC144112336"
                     /coded_by="XM_077645193.1:433..1023"
                     /db_xref="GeneID:144112336"
ORIGIN      
        1 mldfgawcgr pqdnartgwp ghplrlrvhl frpeffadah amvtghksdy vvnlgnwyrs
       61 addlvlhyel rlqtlpegwe rlrvssqaql ksliasapda gidnlfiape alrpfvsscp
      121 qvlqrwrlql mrrefllfkv geepllptse egkellieam ryhlmpewra amagprtsak
      181 qlapkdadth arpsga
//
LOCUS       XP_077501329             196 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112343 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501329
VERSION     XP_077501329.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645203.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..196
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..196
                     /product="uncharacterized protein LOC144112343 isoform X1"
                     /calculated_mol_wt=22179
     Region          <120..163
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     CDS             1..196
                     /gene="LOC144112343"
                     /coded_by="XM_077645203.1:342..932"
                     /db_xref="GeneID:144112343"
ORIGIN      
        1 mldfgawcgr pqdnartgwp ghplrlrvhl frpeffadah amvtghksdy vvnlgnwyrs
       61 addlvlhyel rlqtlpegwe rlrvssqaql ksliasapda gidnlfiape alrpfvsscp
      121 qllqrwrlql mrrefllfkv geepllptse egkellieam ryhlmpewra amagprtsak
      181 qlapkdadth arpsga
//
LOCUS       XP_077501331             196 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112343 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501331
VERSION     XP_077501331.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645205.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..196
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..196
                     /product="uncharacterized protein LOC144112343 isoform X2"
                     /calculated_mol_wt=22159
     Region          <120..163
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     CDS             1..196
                     /gene="LOC144112343"
                     /coded_by="XM_077645205.1:12..602"
                     /db_xref="GeneID:144112343"
ORIGIN      
        1 mpdfgawcgr qqdnartgwp ghplrlrvhl frpefladah amvtghksdy vvnlgnwyrs
       61 addlvlhyel rlqtlpegwe rlrvssqaql ksliasapda gidnlfiape alrpfvsscp
      121 qllqrwrlql mrrefllfkv geepllptse egkellieam ryhlmpewra amagprtsak
      181 qlapkdadth arpsga
//
LOCUS       XP_077501336             173 aa            linear   INV 25-NOV-2025
DEFINITION  TATA-box-binding protein-like [Amblyomma americanum].
ACCESSION   XP_077501336
VERSION     XP_077501336.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645210.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..173
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..173
                     /product="TATA-box-binding protein-like"
                     /calculated_mol_wt=19126
     Region          2..167
                     /region_name="TBP_eukaryotes"
                     /note="eukaryotic TATA box binding protein (TBP): Present
                     in archaea and eukaryotes, TBPs are transcription factors
                     that recognize promoters and initiate transcription. TBP
                     has been shown to be an essential component of three
                     different transcription initiation...; cd04516"
                     /db_xref="CDD:239952"
     Site            order(2,4,31..32,36,38,45,47,51,53,55,57,87,92,94,
                     123..124,129,140,142,144,148)
                     /site_type="other"
                     /note="DNA interaction surface [nucleotide binding]"
                     /db_xref="CDD:239952"
     Site            order(16,23..25,27,32,38,40)
                     /site_type="other"
                     /note="TFIIA interaction surface [polypeptide binding]"
                     /db_xref="CDD:239952"
     Site            order(31,128..129,134,165..166)
                     /site_type="other"
                     /note="NC2 interaction surface [polypeptide binding]"
                     /db_xref="CDD:239952"
     Site            order(104..105,117..121,124,129)
                     /site_type="other"
                     /note="TFIIB interaction surface [polypeptide binding]"
                     /db_xref="CDD:239952"
     CDS             1..173
                     /gene="LOC144112361"
                     /coded_by="XM_077645210.1:78..599"
                     /db_xref="GeneID:144112361"
ORIGIN      
        1 mntmaslslg crldlrkial hapnteydpk rtpalvmrig epramalvfg sgkilcsgak
       61 demkaklasr kfariiqklg fdahflnfki vnivascdvg fmirleglll arrglasyep
      121 elspgltyrp ehprvtlllf psckivftgs kteeevieaf kdiypilqtc rig
//
LOCUS       XP_077501362             542 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112391 [Amblyomma americanum].
ACCESSION   XP_077501362
VERSION     XP_077501362.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645236.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..542
                     /product="uncharacterized protein LOC144112391"
                     /calculated_mol_wt=61244
     Region          214..316
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(267..272,283..285,306..309)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Region          <352..454
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          374..394
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(374,377,390,394)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(379,381,383,385..386,389..390,393,407,409,413..414,
                     417..418,421,435,437,439,441..442,445..446,450)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          400..422
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          402..422
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          414..439
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          430..451
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          459..475
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..542
                     /gene="LOC144112391"
                     /coded_by="XM_077645236.1:307..1935"
                     /db_xref="GeneID:144112391"
ORIGIN      
        1 mrnfklrlln feehyaptat nelrvssrwv ievlkpfqvp kaaagsqssr vpgngrpraa
       61 vepalqdvcr feeematkgy yrwkpayfsd ytnvgkdgsi kerkqestrr pflrkkkqfp
      121 erppsssada lpdhweapas sgrnlpeeps sreelhdekr yngsrmvara dctsdtalad
      181 vkseerkedt sligmsmfef ltsgkstvkg vpvgvftlka ldkglhfgpy hgvkvdvnen
      241 ggctwpvrrc geffvvdgrp eernhwmndv nyspskrrqn lvallsngdv yyrtlrnilp
      301 neellvgyst sfaksllgnp rgpgalkeed vnliplhsll facekcgdlf ttqynmemht
      361 rqkhcqstqg ishcthctyt ssrksdiirh erthadnrny vcnlcqkrfs rpdnlkvhma
      421 ihtrdkpyec gqcgkrfrqh ihakhheqii hfrryplhcp rcgagaenva klrrhtckpl
      481 gttegkegvk ggrgrprlka dgtaptvrpr nttegtplqg gvkrgrgrpr kavaqdvstp
      541 rp
//
LOCUS       XP_077501382             643 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat protein mann-cup [Amblyomma americanum].
ACCESSION   XP_077501382
VERSION     XP_077501382.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645256.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..643
                     /product="ankyrin repeat protein mann-cup"
                     /calculated_mol_wt=72183
     Region          <51..269
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          58..90
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          104..132
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(107..108,111..113,115..116,120,123,132,134,136,
                     140..141,144..146,148..149,153,156,165,167,169,173..174,
                     177,179..180,184,187,196)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          134..165
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          167..196
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          198..228
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          473..>593
                     /region_name="PHA03095"
                     /note="ankyrin-like protein; Provisional"
                     /db_xref="CDD:222980"
     Region          <534..>598
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          554..587
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..643
                     /gene="m-cup"
                     /coded_by="XM_077645256.1:1247..3178"
                     /db_xref="GeneID:144112415"
ORIGIN      
        1 mwitgkidqt sqreaifndl ysecrlappg arlsylvrnk ierltreerk aivsriregc
       61 aplfvackkg nsdiveylit mcgadleqrg vyevtddrsi hfvtplwcaa vagklavvkq
      121 lidyganvns vsdtgstpvr sacfmshrdv vmylvnhgad ilkpnynggt clinsvqsvs
      181 lcrfllrrga dvnaqdvqlk talhyaiqeh rfdttrllle qranpflrsr ygddalqtac
      241 lkgaaqiyey lvttveysaa rraeadelmg ttfldehhdv qsalfywrra qtlrarenvp
      301 kplagtprrp afqmaaeyan adelealahd ldalrnqsll mcerilgaqh kdtvfrlmyr
      361 gaayadslqy qrcidlwqya leirvakdtl lhteagqaaa alvrlyldml dkkragllsh
      421 dqlrledvla tarllvdria dtarllserp vyrrhldnfd kllrvlthll yvlhslpkqn
      481 aaqdadvrrl aaralaqqpr saagdsllhl vvsrantmkp taffeeahah vfpdaplael
      541 llecgadvea vnealstplh vaalrnnfrp dvvqmllrhg ahldrrnang nqphrmlagi
      601 sectlnplqh iglqclaark iverripftg evpvalehfi rih
//
LOCUS       XP_077501388              83 aa            linear   INV 25-NOV-2025
DEFINITION  nuclear hormone receptor family member daf-12-like [Amblyomma
            americanum].
ACCESSION   XP_077501388
VERSION     XP_077501388.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645262.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..83
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..83
                     /product="nuclear hormone receptor family member
                     daf-12-like"
                     /calculated_mol_wt=9290
     Region          11..>83
                     /region_name="NR_DBD_like"
                     /note="DNA-binding domain of nuclear receptors is composed
                     of two C4-type zinc fingers; cl02596"
                     /db_xref="CDD:413390"
     Site            order(13,16,30,33,49,55,65,68)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143512"
     Site            order(23..25,31..32,34,36,39,42,62..63,66,69,80,83)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143512"
     CDS             1..83
                     /gene="LOC144112425"
                     /coded_by="XM_077645262.1:415..666"
                     /db_xref="GeneID:144112425"
ORIGIN      
        1 maaggersel kscgvcgdka fgfnfgaltc esckaffrrn aikdkefrcl fkddcevntr
       61 trrscqrcrl qkcfaigmkk eci
//
LOCUS       XP_077501398             737 aa            linear   INV 25-NOV-2025
DEFINITION  actin-binding LIM protein 2-like [Amblyomma americanum].
ACCESSION   XP_077501398
VERSION     XP_077501398.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645272.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (GKSP01011464.1) annotated
            using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 114 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..737
                     /product="actin-binding LIM protein 2-like"
                     /calculated_mol_wt=83298
     Region          6..57
                     /region_name="LIM1_abLIM"
                     /note="The first LIM domain of actin binding LIM (abLIM)
                     proteins; cd09327"
                     /db_xref="CDD:188713"
     Site            order(6,9,26,29,32,35,53,56)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188713"
     Region          62..116
                     /region_name="LIM2_abLIM"
                     /note="The second LIM domain on actin binding LIM (abLIM)
                     proteins; cd09328"
                     /db_xref="CDD:188714"
     Site            order(65,68,85,88,91,94,113,116)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188714"
     Region          138..189
                     /region_name="LIM3_abLIM"
                     /note="The third LIM domain of actin binding LIM (abLIM)
                     proteins; cd09329"
                     /db_xref="CDD:188715"
     Site            order(138,141,159,162,165,168,185,188)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188715"
     Region          197..253
                     /region_name="LIM4_abLIM"
                     /note="The fourth LIM domain of actin binding LIM (abLIM)
                     proteins; cd09330"
                     /db_xref="CDD:188716"
     Site            order(197,200,218,221,224,227,246,249)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188716"
     Region          702..737
                     /region_name="VHP"
                     /note="Villin headpiece domain; pfam02209"
                     /db_xref="CDD:460493"
     CDS             1..737
                     /gene="LOC144112432"
                     /coded_by="XM_077645272.1:277..2490"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 114 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144112432"
ORIGIN      
        1 mgkvlcelck kkcsgnalrv qdryfhiecf kcavctssla qggfffrdgk yyctgdyqkm
       61 fgtkcagcgq fvegevvtal gntyhqkcfv carcrqqfps gekvtytgke clcskciqip
      121 vvtsqsaeep hingavkcag csddlregqa lialdkqwhi wcfkcthcnv vlhgeymgre
      181 gqpycekdyq kqfgvkcahc drfisgkvlq agenhhfhpt carcskcgdp fgdgeemylq
      241 ggaiwhprcg pgpdgkmedr klddsqkdav aeeqtvgthp pspksfrsas phdslkiqyg
      301 rssspgslld kepmlsdlsr vyaysyltae ptqgylrrpl ephppkspqf hrppdglgnr
      361 rkipikphtk qgmqvlvdti qssaprpksp hmnneepiel ahfpgarppk pteipkierd
      421 dfpappfpyt dperrrrwsg sskeyevdee eeaeeeafee dpqlkkeeee lskiatgigk
      481 vflktvkere kyrawkmthv dprnasrtps ankepayrlr ydnpvnasps rdidrprpwe
      541 edefdrgssy rssmgrsvgt tptynvvssl rtvpkpgygl atksatlpvg grdfmaqdls
      601 fsklekthst efscgksdvs gasdhdgtam nhstsavlrs tpfsdgysgf ryasysphlr
      661 rsmpnvnwhl stdppklypy hllmtsnyrl pvdvdrchle rhlsneefqa lfhmsrlefy
      721 rlpewkrndl krraklf
//
LOCUS       XP_077501406             298 aa            linear   INV 25-NOV-2025
DEFINITION  protein Skeletor, isoforms B/C-like [Amblyomma americanum].
ACCESSION   XP_077501406
VERSION     XP_077501406.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645280.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..298
                     /product="protein Skeletor, isoforms B/C-like"
                     /calculated_mol_wt=31795
     Region          40..137
                     /region_name="DM13"
                     /note="Electron transfer DM13; cl02735"
                     /db_xref="CDD:470661"
     Region          149..247
                     /region_name="DM13"
                     /note="Electron transfer DM13; cl02735"
                     /db_xref="CDD:470661"
     CDS             1..298
                     /gene="LOC144112438"
                     /coded_by="XM_077645280.1:99..995"
                     /db_xref="GeneID:144112438"
ORIGIN      
        1 magrivvlll vsvavgpqgi agayygklig pvktnahgfs gtvyaatens viitdlnydg
       61 egpeaffwvg tgqepdskgh qiadengsmq vlkayknarv lltlpkkitd yqylgiycrk
      121 fganfghvti psgfelpkeq slgrletkah gaeaaevvlk dsatielkqf kydgqgpagf
      181 fvvaptvnar pqeltkllde ngkdtklgry drkdvtlrlp dghhwneyrw fsvfctqavv
      241 sfaditidst vaeklpvhnp ssvapasagt apgvpstlal laclvaalsg gvirartc
//
LOCUS       XP_077501408             944 aa            linear   INV 25-NOV-2025
DEFINITION  protein Skeletor, isoforms B/C-like [Amblyomma americanum].
ACCESSION   XP_077501408
VERSION     XP_077501408.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645282.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..944
                     /product="protein Skeletor, isoforms B/C-like"
                     /calculated_mol_wt=103386
     Region          33..137
                     /region_name="DM13"
                     /note="Electron transfer DM13; cl02735"
                     /db_xref="CDD:470661"
     Region          146..254
                     /region_name="DM13"
                     /note="Electron transfer DM13; cl02735"
                     /db_xref="CDD:470661"
     Region          277..382
                     /region_name="DM13"
                     /note="Electron transfer DM13; cl02735"
                     /db_xref="CDD:470661"
     Region          392..498
                     /region_name="DM13"
                     /note="Electron transfer DM13; cl02735"
                     /db_xref="CDD:470661"
     Region          525..672
                     /region_name="DOMON_DOH"
                     /note="DOMON-like domain of copper-dependent
                     monooxygenases and related proteins; cd09631"
                     /db_xref="CDD:187689"
     Site            order(553,555..556,571..573,644,646,654,671)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:187689"
     CDS             1..944
                     /gene="LOC144112447"
                     /coded_by="XM_077645282.1:93..2927"
                     /db_xref="GeneID:144112447"
ORIGIN      
        1 magihvlall lacalqgara qyygkligrl etlqhrvsgl vyaagedsfy lvdfsydggg
       61 pdaffwagts tspdssgeil pdengarqrl rgynrenili kldnnktlrd yrylavycrr
      121 ysvnfgwvri pddfelpaaq dlgflsgihg vradpvvlld srtvhltnfq ydgagpdahf
      181 vvgrgeinga ngltiadeng sfeklrrypg knvtlrlreg gswdqyewls vyclkasqdf
      241 ahirlptpdk ltvpvrmaqe asggrylgkl lgnldtgvhg vggkvyiasg dslvledfsy
      301 dgagpdayfl vgttdrpdgs gtmlknevgs dkplpayskk tivlklpagt kitdfkwfsv
      361 ycrkaktsfa hvdipaegld ypkpatvasn isgahntraq avivedkktl llknfyydgs
      421 apdafflagk gdrprpdgtk ipdesgrark lrgyvnadvr ltlpgeltvd didwfavyci
      481 tytetfiqar fpknlnvpps lellekeaqq spsvtpqfdn cetiisnriq vgwhletdti
      541 tfhvraqarp gmwtafgvsg ekdksvmvga dvavifvrgp nnqvsvidyy lsskaqcssn
      601 ggvcpdtkvg gtedlkliss syangivdvv ysrklatads ndktiattgr vavvaaqgpt
      661 nkdrpdtvly htmvvtrddv llqfnrnaqr scppmapilp ppaggrtgfg grdiwksfni
      721 ttfraqigpt ggsrgyervt gqsgwgisww ineelipvlh vergktytfv veggvdasns
      781 aryhpfyitd nskgggsked pavlgkkghr llagvvldar nkvdvskgtg rycewthktv
      841 dksdesstwe dfkqtlnlqc dvgqpgtftw tpdkdtpdvv yyqcfthyyl gwkimvtdpd
      901 qpergrgkdr mesdrgedrm esgagpttal lpllllgviv skaa
//
LOCUS       XP_077501416             155 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112457 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501416
VERSION     XP_077501416.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645290.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..155
                     /product="uncharacterized protein LOC144112457 isoform X1"
                     /calculated_mol_wt=16478
     CDS             1..155
                     /gene="LOC144112457"
                     /coded_by="XM_077645290.1:147..614"
                     /db_xref="GeneID:144112457"
ORIGIN      
        1 maplppfsek assagfgcrs lrrrlcfrim hgstkcdlcl rrgavcrfvl rvcvccasgv
       61 stipvalvpl dggairlnnp davqsalqai splvlkicda rqygrggvlc rsadqafimd
      121 llkctsfgsq slwdrvhmrs etgcggglgr waiss
//
LOCUS       XP_077501424             100 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112457 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501424
VERSION     XP_077501424.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645298.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..100
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..100
                     /product="uncharacterized protein LOC144112457 isoform X2"
                     /calculated_mol_wt=10834
     CDS             1..100
                     /gene="LOC144112457"
                     /coded_by="XM_077645298.1:46..348"
                     /db_xref="GeneID:144112457"
ORIGIN      
        1 mprlagaksc maphsatcvy dvepfadlfy vcayvaravg srphalgnrl rrrprtvghl
       61 qltrhtvrwt vppisgvpaa cpfprrsrpp lsasiggwtd
//
LOCUS       XP_077501444             310 aa            linear   INV 25-NOV-2025
DEFINITION  GDP-D-glycero-alpha-D-manno-heptose dehydrogenase-like [Amblyomma
            americanum].
ACCESSION   XP_077501444
VERSION     XP_077501444.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645318.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..310
                     /product="GDP-D-glycero-alpha-D-manno-heptose
                     dehydrogenase-like"
                     /calculated_mol_wt=33282
     Region          6..297
                     /region_name="WcaG"
                     /note="Nucleoside-diphosphate-sugar epimerase [Cell
                     wall/membrane/envelope biogenesis]; COG0451"
                     /db_xref="CDD:440220"
     CDS             1..310
                     /gene="LOC144112491"
                     /coded_by="XM_077645318.1:67..999"
                     /db_xref="GeneID:144112491"
ORIGIN      
        1 mslpevlvtg gagylgsclv plllergwrv avfdtfargl gpllpvvdrv rlvrgdvrdg
       61 aalaaalrgv hsvvhlaavv gypacsrdpa laedvnvggt ralleqlspe qqlvfastgs
      121 cygavgpava cteetplspl svygrtkaea ealvlerrgv alrlatvfgv aprmrvdllv
      181 ndlsrralrd galevyepgf wrsllhvrda arafalaler ydemrgqafn vgddalnatk
      241 eqlarlicer aggarlvlgg pgsdqdrrdy rvsherirql gfrasvtleq gvdellrvlp
      301 lmaphelctn
//
LOCUS       XP_077501450             738 aa            linear   INV 25-NOV-2025
DEFINITION  mitogen-activated protein kinase kinase kinase 13-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077501450
VERSION     XP_077501450.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645324.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..738
                     /product="mitogen-activated protein kinase kinase kinase
                     13-like isoform X1"
                     /calculated_mol_wt=82102
     Region          119..355
                     /region_name="STKc_MAP3K12_13"
                     /note="Catalytic domain of the Serine/Threonine Kinases,
                     Mitogen-Activated Protein Kinase Kinase Kinases 12 and 13;
                     cd14059"
                     /db_xref="CDD:270961"
     Site            order(119..123,127,138,140,162,178..181,185,187,224,226,
                     228..229,231,242,245,260..263)
                     /site_type="active"
                     /db_xref="CDD:270961"
     Site            order(119..123,127,138,140,162,178..181,185,224,226,
                     228..229,231,242)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270961"
     Site            order(123,185,187,224,226,228,245,260..263)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270961"
     Site            241..263
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270961"
     CDS             1..738
                     /gene="LOC144112497"
                     /coded_by="XM_077645324.1:118..2334"
                     /db_xref="GeneID:144112497"
ORIGIN      
        1 msggvvadae astelqslpp altasidred lgastdkedt fgkgvlciqe evrtealsat
       61 elevayreag gaasaggwld gllgclrpvw silhktddrq gpgdsweipf eaisqlqfvg
      121 sgaqgavfvg qlrdetvavk kvtslaetdi rhlrklnhpn ivafkgvctq epcfcivmey
      181 cpygqlydal kngriippat vvewskhias gmnylhsrni ihrdlkspni lisyndvlki
      241 sdfgtsrqwc erstkmsfag tvawmapevi rnepcsekvd iwsygvvmwe llncetpykd
      301 vdsnaiiwgv gnnslhlpvp atcpdgfrll mrqcwspkpr nrpsfrhilm hldiaaveil
      361 stpkesyfaa qagwkeeirr ymqsikqdsq hgpqgeqeli rrrreelrha qdirlhyerk
      421 lertnnlyme ftacllqleq rereiirreq slemgpgyrp ykkrivrpli kaqerfgkkr
      481 syrlptdpps pepleqagsp stkarsrrat hrrsgsgsss ccrtvmvdse tqtdalells
      541 essspslasp vlpestpqsd dafpvveedl nsnrapvqts tadtpceqqr pvlpppsrhk
      601 ramtdsdesp srahsslgrg sqdlttseee gevddeytar qgqlskmrqa vsgqsistls
      661 segnlseeeg ntsecsscpp aellsslsnp dvrdcgrtpr rpplrlkrnp lptisdtyss
      721 psqshvnset ghtettvw
//
LOCUS       XP_077501453             720 aa            linear   INV 25-NOV-2025
DEFINITION  mitogen-activated protein kinase kinase kinase 13-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077501453
VERSION     XP_077501453.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645327.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..720
                     /product="mitogen-activated protein kinase kinase kinase
                     13-like isoform X2"
                     /calculated_mol_wt=80199
     Region          119..355
                     /region_name="STKc_MAP3K12_13"
                     /note="Catalytic domain of the Serine/Threonine Kinases,
                     Mitogen-Activated Protein Kinase Kinase Kinases 12 and 13;
                     cd14059"
                     /db_xref="CDD:270961"
     Site            order(119..123,127,138,140,162,178..181,185,187,224,226,
                     228..229,231,242,245,260..263)
                     /site_type="active"
                     /db_xref="CDD:270961"
     Site            order(119..123,127,138,140,162,178..181,185,224,226,
                     228..229,231,242)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270961"
     Site            order(123,185,187,224,226,228,245,260..263)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270961"
     Site            241..263
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270961"
     CDS             1..720
                     /gene="LOC144112497"
                     /coded_by="XM_077645327.1:116..2278"
                     /db_xref="GeneID:144112497"
ORIGIN      
        1 msggvvadae astelqslpp altasidred lgastdkedt fgkgvlciqe evrtealsat
       61 elevayreag gaasaggwld gllgclrpvw silhktddrq gpgdsweipf eaisqlqfvg
      121 sgaqgavfvg qlrdetvavk kvtslaetdi rhlrklnhpn ivafkgvctq epcfcivmey
      181 cpygqlydal kngriippat vvewskhias gmnylhsrni ihrdlkspni lisyndvlki
      241 sdfgtsrqwc erstkmsfag tvawmapevi rnepcsekvd iwsygvvmwe llncetpykd
      301 vdsnaiiwgv gnnslhlpvp atcpdgfrll mrqcwspkpr nrpsfrhilm hldiaaveil
      361 stpkesyfaa qagwkeeirr ymqsikqdsq hgpqgeqeli rrrreelrha qdirlhyerk
      421 lertnnlyme ftacllqleq rereiirreq slemgpgyrp ykkrivrpli kaqerfgkkr
      481 syrlptdpps pepleqagsp stkarsrrat hrrsgsgsss ccrtvmvdse tqtdalells
      541 essspslasp vlpestpqsd dafpvveedl nsnrapvqts tadtpceqqr pvlpppsrhk
      601 ramtdsdesp srahsslgrg sqdlttseee gevddeytar qgsegnlsee egntsecssc
      661 ppaellssls npdvrdcgrt prrpplrlkr nplptisdty sspsqshvns etghtettvw
//
LOCUS       XP_077501477             776 aa            linear   INV 25-NOV-2025
DEFINITION  N-acetylneuraminate (7)9-O-acetyltransferase [Amblyomma
            americanum].
ACCESSION   XP_077501477
VERSION     XP_077501477.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645351.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..776
                     /product="N-acetylneuraminate (7)9-O-acetyltransferase"
                     /calculated_mol_wt=88238
     Region          298..760
                     /region_name="Cas1_AcylT"
                     /note="10 TM Acyl Transferase domain found in Cas1p;
                     pfam07779"
                     /db_xref="CDD:462263"
     CDS             1..776
                     /gene="LOC144112545"
                     /coded_by="XM_077645351.1:110..2440"
                     /db_xref="GeneID:144112545"
ORIGIN      
        1 mveegrhint ssefihrvna knckivacfv vvgfvlyhgv ihltygidtc kwllsdgrfq
       61 gyhvwqpygc mlhtytnaes rmclryiayw ggknhivflg dsrirqlywa fvqqvqpspl
      121 aapptqahqd lryeekdlrl tveflwrplv dssmraagar wlqgggptll vlgsgtwair
      181 qsngsraala eyaanvsrlv plldrlanss arvlwmlqdp vqadrlhpsr raitnelida
      241 ynqaamhalr hssvevwssi rlisegypsd sldglhtgpl avnyaiqilt nmycndhmny
      301 ndgtccsspe pvtwlqtitf aafgvilvlt ltlavrqqlw paadsalqgv lqplsrlgli
      361 mayfflcdrt nffmkenkyy thlnfflpva yvfalglfft eesqqtqvlh rdqtdewkgw
      421 mqlvllvyhm tgasqvlpvy vhvralvsay lflagyghfs yfwhqadfgl lrlarvlfrt
      481 nllvvllclc mnrpyqfyyf vplvsfwfmv vaatlgslpr vsaasaeanp lhhlyvvlkf
      541 vglfsvltvl ymsevffeki fvtrpwkalf vttddsikew wfrwkidrys vasgmlfgfa
      601 hyllrqyrvi gdnhhgnlfs rglsltvafg allglgfytt fafvcrskpd cnevhayisf
      661 vpilgyvilr nisgllrtry stffawfgki slelfiaqyh iwlaadthgv lvlvpgypvl
      721 nvmitslvlv cvahevhslt arlmplavps dwkylvrnla vfglllvpig ihdgmf
//
LOCUS       XP_077501488             704 aa            linear   INV 25-NOV-2025
DEFINITION  atrial natriuretic peptide-converting enzyme-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077501488
VERSION     XP_077501488.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645362.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..704
                     /product="atrial natriuretic peptide-converting
                     enzyme-like isoform X1"
                     /calculated_mol_wt=76307
     Region          218..333
                     /region_name="CRD_FZ"
                     /note="CRD_domain cysteine-rich domain, also known as Fz
                     (frizzled) domain; cd07066"
                     /db_xref="CDD:143549"
     Site            order(225,227,229,232..234)
                     /site_type="other"
                     /note="putative Wnt binding site [polypeptide binding]"
                     /db_xref="CDD:143549"
     Region          <345..363
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(346,349,353,359..360)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            356..360
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          366..400
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(371,379,390..391)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(383,386,390,396..397)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            393..397
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          509..677
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cl21584"
                     /db_xref="CDD:473915"
     Site            order(545,588,658)
                     /site_type="active"
                     /db_xref="CDD:238113"
     CDS             1..704
                     /gene="LOC144112552"
                     /coded_by="XM_077645362.1:30..2144"
                     /db_xref="GeneID:144112552"
ORIGIN      
        1 mpapahspfa rvtkrgghri tftqrragct lagclcasla alcltlgaaa gvyygvrfle
       61 gqrtaervyr gaftvlegdk fseslsnsla ddfvrkaday rakldrlfns saafrgtevi
      121 afqrredrel tvhfnvhfsp ygpvdagely lamadellrg rlgvfqglkv dpeslslqer
      181 rataslpesw tprrrypgyl aglstsrpav grqaellcep ltldhcrasl pynstaypna
      241 vghatardvy rdlvayrqla dsechplaae lvcrllqpqc ddaerrvvlp crefcqefws
      301 scqqqmplai aghidcgrlp eysaagscht kpgcanelka rgkeshicdg vvdcpdfsde
      361 tscdncgpgl lhcgdrqcvd iaqrcdarld cdngadeqnc lslpelggyl rayqrghfrk
      421 vcvdglpegr rrdsllkqla dtacaalnlg ppsrvelhhd gnssdsylql ldppglrfss
      481 pgacrsdlvv yllcsppecg qssalpttqe vrhgdwpwhv mllqagrhvc dgtlvadrwv
      541 ltagaclpqg rdpsgewqar lgsvrkesas pfdvraaiaa vlrsadgvla llrldaavpq
      601 sravrpaclp rtpppqagrc atlaygvkgd qleqvnleas ecgpavfcgr qascvhesla
      661 grqlmcwhgd rwqlagvaae pcsggaqrfh glgphlawae aals
//
LOCUS       XP_077501495             622 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112552 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501495
VERSION     XP_077501495.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645369.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..622
                     /product="uncharacterized protein LOC144112552 isoform X2"
                     /calculated_mol_wt=68655
     Region          218..333
                     /region_name="CRD_FZ"
                     /note="CRD_domain cysteine-rich domain, also known as Fz
                     (frizzled) domain; cd07066"
                     /db_xref="CDD:143549"
     Site            order(225,227,229,232..234)
                     /site_type="other"
                     /note="putative Wnt binding site [polypeptide binding]"
                     /db_xref="CDD:143549"
     Region          <345..363
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(346,349,353,359..360)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            356..360
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          366..400
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(371,379,390..391)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(383,386,390,396..397)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            393..397
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     CDS             1..622
                     /gene="LOC144112552"
                     /coded_by="XM_077645369.1:30..1898"
                     /db_xref="GeneID:144112552"
ORIGIN      
        1 mpapahspfa rvtkrgghri tftqrragct lagclcasla alcltlgaaa gvyygvrfle
       61 gqrtaervyr gaftvlegdk fseslsnsla ddfvrkaday rakldrlfns saafrgtevi
      121 afqrredrel tvhfnvhfsp ygpvdagely lamadellrg rlgvfqglkv dpeslslqer
      181 rataslpesw tprrrypgyl aglstsrpav grqaellcep ltldhcrasl pynstaypna
      241 vghatardvy rdlvayrqla dsechplaae lvcrllqpqc ddaerrvvlp crefcqefws
      301 scqqqmplai aghidcgrlp eysaagscht kpgcanelka rgkeshicdg vvdcpdfsde
      361 tscdncgpgl lhcgdrqcvd iaqrcdarld cdngadeqnc lslpelggyl rayqrghfrk
      421 vcvdglpegr rrdsllkqla dtacaalnlg ppsrvelhhd gnssdsylql ldppglrfss
      481 pgacrsdlvv yllcsppgvp nwaeaddsar grtatllegc vvlslilagr sggkrhnhgg
      541 rsqtaikqyf flgqycatww ysrrswtsqp ylgvcvhtht rthartrtht hkptvtetkv
      601 hrgtffcsfy vqcqsieehy fd
//
LOCUS       XP_077501501             673 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112566 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501501
VERSION     XP_077501501.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645375.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..673
                     /product="uncharacterized protein LOC144112566 isoform X1"
                     /calculated_mol_wt=76010
     Region          593..612
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(597,599,601,603..604,607..608,611,627,629,633..634,
                     637..638,641,655,657,659,661..662,665..666,669)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          <604..670
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          620..642
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          648..670
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          650..670
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..673
                     /gene="LOC144112566"
                     /coded_by="XM_077645375.1:148..2169"
                     /db_xref="GeneID:144112566"
ORIGIN      
        1 msvhvkdpqv veiipepstt apnssflslp llflplrrgs gvkrymiqil rhflspkktn
       61 yyyfstclpp qriilvasks rksppqrivl vaskplpgqy arvdasshff sqltrlggvq
      121 yderssgypn lkmriqdhfk mrllnfeehc altatnepva ssrwvievik pfqmpgaaar
      181 nessrvpgng rphravepas qqvlrfeeem atkgyycwkp ayfsdetwrk llddikkryt
      241 tvrkdgsike rkqattnrpf lrkrcpgyap aaklgpssde peivvlddss scdvvfkthn
      301 esppsspdsv ilvkwekgik scrpdleqeg aseewlrceq rvptgeeepm vvepselqdq
      361 rqlaegldsi vdgsqheanv patqllpmnt khdliqcenr eyrdlqgdqq hhitqgnqet
      421 sgqlqdqrqp aegldsivdg sqheanvpat qllpmntehd liqcenrecr dlqgdqqhli
      481 kqgnqetsgq fsdqrqpaeg ldpiqcenre crdlhgdqqh hikqqtpphv dtvtrfcsst
      541 egqsastaig sanletgprc sssvlavpeg kepggqqgtt rvkggavhic pypdcgrrht
      601 krrhfeahlr shtgekpyec gwancgrkfa rsdaltrhyr khtgerlylc ewcgrtfsrn
      661 dnllrhqkth rge
//
LOCUS       XP_077501504             606 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112566 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501504
VERSION     XP_077501504.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645378.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..606
                     /product="uncharacterized protein LOC144112566 isoform X2"
                     /calculated_mol_wt=68520
     Region          526..545
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(530,532,534,536..537,540..541,544,560,562,566..567,
                     570..571,574,588,590,592,594..595,598..599,602)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          <537..603
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          553..575
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          581..603
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          583..603
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..606
                     /gene="LOC144112566"
                     /coded_by="XM_077645378.1:148..1968"
                     /db_xref="GeneID:144112566"
ORIGIN      
        1 msvhvkdpqv veiipepstt apnssflslp llflplrrgs gvkrymiqil rhflspkktn
       61 yyyfstclpp qriilvasks rksppqrivl vaskplpgqy arvdasshff sqltrlggvq
      121 yderssgypn lkmriqdhfk mrllnfeehc altatnepva ssrwvievik pfqmpgaaar
      181 nessrvpgng rphravepas qqvlrfeeem atkgyycwkp ayfsdetwrk llddikkryt
      241 tvrkdgsike rkqattnrpf lrkrcpgyap aaklgpssde peivvlddss scdvvfkthn
      301 esppsspdsv ilvkwekgik scrpdleqeg aseewlrceq rvptgeeepm vvepselqdq
      361 rqlaegldsi vdgsqheanv patqllpmnt khdliqcenr eyrdlqgdqq hhitqgnqet
      421 sgqfsdqrqp aegldpiqce nrecrdlhgd qqhhikqqtp phvdtvtrfc sstegqsast
      481 aigsanletg prcsssvlav pegkepggqq gttrvkggav hicpypdcgr rhtkrrhfea
      541 hlrshtgekp yecgwancgr kfarsdaltr hyrkhtgerl ylcewcgrtf srndnllrhq
      601 kthrge
//
LOCUS       XP_077501511             777 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112584 [Amblyomma americanum].
ACCESSION   XP_077501511
VERSION     XP_077501511.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645385.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..777
                     /product="uncharacterized protein LOC144112584"
                     /calculated_mol_wt=86119
     Region          257..355
                     /region_name="MULE"
                     /note="MULE transposase domain; pfam10551"
                     /db_xref="CDD:463145"
     CDS             1..777
                     /gene="LOC144112584"
                     /coded_by="XM_077645385.1:20..2353"
                     /db_xref="GeneID:144112584"
ORIGIN      
        1 maasslpfec eldsdncven vscfrvalnt dgdayewlts ygvatrttwi vdwevakpkr
       61 iafhkkwkcq hsnrnkitgq tatncpafvd ikiknvtrdt rkrdpflkra tplraivkvr
      121 ddhnhaldca dalrllrtta dtravfhtyf hdgltpaqai amhqqkleae ddaaeklasg
      181 avnpsagtvy ywyrlwrdhh ygsavdpvsk lsekapcyld kgadvrttrs edgtcwavlv
      241 vtqvmkrtqg lsaakeiifv dstascdesq ssltvvltat avgamplavl lhssqssesy
      301 kaafgllkls yptcfggtha pqafmtdnsa aekaalqatw pqgkqllchf hvaqaewrwl
      361 hasrdtsrdd krdlmttfqk imyastandl ntaiaemkak shkgyvarvl gflerkaewv
      421 llyrsgmltr ghntnnfaea sirvlkdvvl grhkafnvva lvdlitslwe ghlqkrllkh
      481 aynrvpshkl lydkllermp enaaasirtl gsnlfevpsc kedgklyevw qdvgtctcra
      541 gqqgafckhq alvhstyggg fpnapvlstq drhqlawlal gdkcqnpaff rdfresawdq
      601 pgsssgepgn epvqpqpqpl vqdatslpqd tmeveeqlav agpssnhqcr edaaevlkni
      661 teelwrqysl aadnpfyltl lqrdlallkr artepqvvam hlastaakas slrrgriikv
      721 qptglarrrp gvtrgaarvh agrplktags rktkrlhslh lsvkdavpha kshgtgh
//
LOCUS       XP_077501515             168 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112593 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501515
VERSION     XP_077501515.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645389.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..168
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..168
                     /product="uncharacterized protein LOC144112593 isoform X1"
                     /calculated_mol_wt=19182
     CDS             1..168
                     /gene="LOC144112593"
                     /coded_by="XM_077645389.1:8..514"
                     /db_xref="GeneID:144112593"
ORIGIN      
        1 matstaavsa lpggwqwfaw mlflhrtlph samqqfpysf ctalhtsniq dqpahvckym
       61 rkvrrcgefv vdgcpqernh wmnvnylpsk rrqnigallf ngdiyrkkvp hgrpgngipy
      121 pavepasrev wrfeeglryr csclrhskap rtagsvtcre flladfll
//
LOCUS       XP_077501522             160 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112593 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501522
VERSION     XP_077501522.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645396.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..160
                     /product="uncharacterized protein LOC144112593 isoform X2"
                     /calculated_mol_wt=18539
     CDS             1..160
                     /gene="LOC144112593"
                     /coded_by="XM_077645396.1:12..494"
                     /db_xref="GeneID:144112593"
ORIGIN      
        1 mqswrrcsve gtslvfynas tcaaaldrky flwklllalk fcsfctalht sniqdqpahv
       61 ckymrkvrrc gefvvdgcpq ernhwmnvny lpskrrqnig allfngdiyr kksiiqvnsl
      121 krcrhnlrrs tvqvagqpit hvlhfgcfkk ktqkirskmv
//
LOCUS       XP_077501529             144 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112593 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501529
VERSION     XP_077501529.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645403.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..144
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..144
                     /product="uncharacterized protein LOC144112593 isoform X3"
                     /calculated_mol_wt=16658
     CDS             1..144
                     /gene="LOC144112593"
                     /coded_by="XM_077645403.1:12..446"
                     /db_xref="GeneID:144112593"
ORIGIN      
        1 mqswrrcsve gtslvfynas tcaaaldrky flwklllalk fcsfctalht sniqdqpahv
       61 ckymrkvrrc gefvvdgcpq ernhwmnvny lpskrrqnig allfngdiyr kkvphgrpgn
      121 gipypavepa srevwrfeeg lryr
//
LOCUS       XP_077501536             103 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112611 [Amblyomma americanum].
ACCESSION   XP_077501536
VERSION     XP_077501536.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645410.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..103
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..103
                     /product="uncharacterized protein LOC144112611"
                     /calculated_mol_wt=10677
     CDS             1..103
                     /gene="LOC144112611"
                     /coded_by="XM_077645410.1:33..344"
                     /db_xref="GeneID:144112611"
ORIGIN      
        1 mlelltsdks tvkgvlvgvf tlkalpegph larvtesrwt askmaaapgr lpaagfaarp
       61 tssatatsgp gpalfcalks ceswdflsls fclvlqdkkl vdl
//
LOCUS       XP_077501548             678 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112617 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501548
VERSION     XP_077501548.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645422.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..678
                     /product="uncharacterized protein LOC144112617 isoform X1"
                     /calculated_mol_wt=75264
     Region          <44..>240
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..678
                     /gene="LOC144112617"
                     /coded_by="XM_077645422.1:114..2150"
                     /db_xref="GeneID:144112617"
ORIGIN      
        1 mmekedrdeg dqdprrndat keficpigme kyiafkravi qaheinesmd aldkasdeie
       61 kkvnelekts ktwqeesmks ereycaglaq leavrkilan crvvqdqhva eweeaeiklr
      121 dlkgslkqqe eviqvakkaa ndsvslfesr cndlqarvpq veeclkltlq qqdehhtrlk
      181 ralgwitast klsqreavta sqptgfavcq sgneeqqllp rvdecpklrl qkedkqrrlr
      241 kralgwitps tklskreavt asqptgfavr qsandeqqlr egkeevtaeq lsamiaemki
      301 pklispipsp arderpeesl elpaappepe qmiskqpgda vgpakgeqrg qasmghrtsv
      361 swnpqehsaq gwiiastkls krqavtapqp tgsavgrsgn deqqlrdpvg pakrrlqgqa
      421 smghctsvsw npqehsaqgw itastklskr eavtapqptg savcrsgnde qqlregkeev
      481 taeqlramfa emkipklisp ipsparderp eeslelpaap pepeqmiskq pvpqveecpk
      541 lklqkedkqr rfrkraldwf eyvkkwegav aapqpsgfaa yrsgsdeqql wdpvgpakrr
      601 lqgqasmghc tsvswnpqeh saqdwtmatt klsqrdvaas qatgfavcrs gndeqqqcae
      661 yvtsglpagt spgmastt
//
LOCUS       XP_077501554             675 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112617 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501554
VERSION     XP_077501554.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645428.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..675
                     /product="uncharacterized protein LOC144112617 isoform X2"
                     /calculated_mol_wt=74969
     Region          <44..>240
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..675
                     /gene="LOC144112617"
                     /coded_by="XM_077645428.1:114..2141"
                     /db_xref="GeneID:144112617"
ORIGIN      
        1 mmekedrdeg dqdprrndat keficpigme kyiafkravi qaheinesmd aldkasdeie
       61 kkvnelekts ktwqeesmks ereycaglaq leavrkilan crvvqdqhva eweeaeiklr
      121 dlkgslkqqe eviqvakkaa ndsvslfesr cndlqarvpq veeclkltlq qqdehhtrlk
      181 ralgwitast klsqreavta sqptgfavcq sgneeqqllp rvdecpklrl qkedkqrrlr
      241 kralgwitps tklskreavt asqptgfavr qsandeqqlr egkeevtaeq lsamiaemki
      301 pklispipsp arderpeesl elpaappepe qmiskqpgda vgpakgeqrg qasmghrtsv
      361 swnpqehsaq gwiiastkls krqavtapqp tgsavgrsgn deqqlrdpvg pakrrlqgqa
      421 smghctsvsw npqehsaqgw itastklskr eavtapqptg savcrsgnde qqlregkeev
      481 taeqlramfa emkipklisp ipsparderp eeslelpaap pepeqmiskq pvpqveecpk
      541 lklqkedkqr rfrkraldwf eyvkkwegav aapqpsgfaa yrsgsdeqql wdpvgpakrr
      601 lqgqasmghc tsvswnpqeh saqdwtmatt klsqrdvaas qatgfavcrs gndeqqqcae
      661 yvtsglpagt spgpv
//
LOCUS       XP_077501555             643 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112617 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501555
VERSION     XP_077501555.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645429.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..643
                     /product="uncharacterized protein LOC144112617 isoform X3"
                     /calculated_mol_wt=71554
     Region          <44..>240
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..643
                     /gene="LOC144112617"
                     /coded_by="XM_077645429.1:114..2045"
                     /db_xref="GeneID:144112617"
ORIGIN      
        1 mmekedrdeg dqdprrndat keficpigme kyiafkravi qaheinesmd aldkasdeie
       61 kkvnelekts ktwqeesmks ereycaglaq leavrkilan crvvqdqhva eweeaeiklr
      121 dlkgslkqqe eviqvakkaa ndsvslfesr cndlqarvpq veeclkltlq qqdehhtrlk
      181 ralgwitast klsqreavta sqptgfavcq sgneeqqllp rvdecpklrl qkedkqrrlr
      241 kralgwitps tklskreavt asqptgfavr qsandeqqlr egkeevtaeq lsamiaemki
      301 pklispipsp arderpeesl elpaappepe qmiskqpgda vgpakgeqrg qasmghrtsv
      361 swnpqehsaq gwiiastkls krqavtapqp tgsavgrsgn deqqlrdpvg pakrrlqgqa
      421 smghctsvsw npqehsaqgw itastklskr eavtapqptg savcrsgnde qqlrdpvgpa
      481 krrlqgqasm ghctsvswnp qehsaqviik wlwlmphgrk vvpvvdvlvt ladkdlqftf
      541 reeplshfcq dtgsmpihrt crfrvstagl ieqvqaqrtv hvdkgvqass ahcewsppve
      601 elsakeaeqm vlqrqdsgqf leeaaekttp ekeapvekga elv
//
LOCUS       XP_077501556             561 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112617 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077501556
VERSION     XP_077501556.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645430.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..561
                     /product="uncharacterized protein LOC144112617 isoform X4"
                     /calculated_mol_wt=61820
     Region          <44..>240
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..561
                     /gene="LOC144112617"
                     /coded_by="XM_077645430.1:114..1799"
                     /db_xref="GeneID:144112617"
ORIGIN      
        1 mmekedrdeg dqdprrndat keficpigme kyiafkravi qaheinesmd aldkasdeie
       61 kkvnelekts ktwqeesmks ereycaglaq leavrkilan crvvqdqhva eweeaeiklr
      121 dlkgslkqqe eviqvakkaa ndsvslfesr cndlqarvpq veeclkltlq qqdehhtrlk
      181 ralgwitast klsqreavta sqptgfavcq sgneeqqllp rvdecpklrl qkedkqrrlr
      241 kralgwitps tklskreavt asqptgfavr qsandeqqlr egkeevtaeq lsamiaemki
      301 pklispipsp arderpeesl elpaappepe qmiskqpgda vgpakgeqrg qasmghrtsv
      361 swnpqehsaq gwiiastkls krqavtapqp tgsavgrsgn deqqlrdpvg pakrrlqgqa
      421 smghctsvsw npqehsaqgw itastklskr eavtapqptg savcrsgnde qqlrdpvgpa
      481 krrlqgqasm ghctsvswnp qehsaqdwtm attklsqrdv aasqatgfav crsgndeqqq
      541 caeyvtsglp agtspgmast t
//
LOCUS       XP_077501557             378 aa            linear   INV 25-NOV-2025
DEFINITION  neuropeptide F receptor-like [Amblyomma americanum].
ACCESSION   XP_077501557
VERSION     XP_077501557.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645431.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..378
                     /product="neuropeptide F receptor-like"
                     /calculated_mol_wt=42103
     Region          47..338
                     /region_name="7tmA_NPYR-like"
                     /note="neuropeptide Y receptors and related proteins,
                     member of the class A family of seven-transmembrane G
                     protein-coupled receptors; cd15203"
                     /db_xref="CDD:320331"
     Region          47..72
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320331"
     Region          79..104
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320331"
     Site            order(101,104..105,117..122,124..125,128,173,175..179,209,
                     212..214,216..218,220..221,283,286..287,289..290,293,306,
                     308..310,313,316..317)
                     /site_type="other"
                     /note="putative peptide ligand binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:320331"
     Region          117..149
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320331"
     Region          160..181
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320331"
     Region          209..238
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320331"
     Region          263..293
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320331"
     Region          306..331
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320331"
     CDS             1..378
                     /gene="LOC144112632"
                     /coded_by="XM_077645431.1:133..1269"
                     /db_xref="GeneID:144112632"
ORIGIN      
        1 mnlssvadln ltlysgtfnf slgraldilq qhyrsdrvfs gsteagivaa yvflitsgvf
       61 cnllvsgvil shakvrtnrn lfvvnlsvsd talclfcmpf tvysltrktw plgellcklv
      121 pcfqsatvfv saatvsaiaf drhqtvvtvn vqrpedgrrr vvwymaaiwi fsvalslpmc
      181 lsqslqqvgl srrymyekcv eqwpspgakq lytlvtlliq fvipttalvv thfrikshls
      241 csgsgasapd krgyserler elrrnrratm vllnvsvvfa vswlpwnamn lltdfhlegi
      301 spqrlylifa vchmvamssa ttnpimygwl ntnirrellg vrnrvglvls slrasnsnlh
      361 tsmddnsrta vtvdkgrl
//
LOCUS       XP_077501558            1482 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112633 [Amblyomma americanum].
ACCESSION   XP_077501558
VERSION     XP_077501558.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645432.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1482
                     /product="uncharacterized protein LOC144112633"
                     /calculated_mol_wt=167279
     Region          518..1078
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1482
                     /gene="LOC144112633"
                     /coded_by="XM_077645432.1:1881..6329"
                     /db_xref="GeneID:144112633"
ORIGIN      
        1 meppakpllp gpmpltsasc ardrqdncre malrvhclds gwgtptasql rrllglqcav
       61 gqvvagdcge vevyespnwt anvtlklers pkdsfmhivl kwcdqyqngs nngaaagksg
      121 ppewwlasee leefcdpyst qiatehkdvp vnafalgcfl gyntfvahfd stytmpppps
      181 plsstkssss hsgksmsvkr gkkgrgakss cpsdgtsssa ktacpnselt spegfkvtqc
      241 nahfehcfng lylyfvdvrk rsstrfrllv rysticriil rktsptctiv ylqllhppll
      301 ykvcagnsat sqcncegdyl pqfdlsdttp wdravefgeg pfkcgadvlg rsrvmrltls
      361 gprhwaplcn lilrtrknvq vfyapvrelq arelkavarp svqdfgvmya llsvwsesfq
      421 vtdelamdaq scretllela ahaeecpral eealfeiyfs lsqgkvfwfr salehlyfry
      481 rahrqcidvf tgsviaqelp shmvkvrkvi ltptrrvymp pqlvcksrll qhcdpdyalr
      541 ivvrdddckl isfslgackd dflsavikpp llsglkiggr rfmflgcsts qlrnhgiwfy
      601 ahdnqgrtae qirdgigdls tipsvpkfma rlgqafsqsl glltvprqnt svepdichaa
      661 pgrssdgshr eyifsdgigr ishgllrkvh haleleegee pcaiqirygg ckgmllldpt
      721 lsgcrivfre smrkfhsdhs dlyvlktskp rvlylnrpmi tileqsgika evflmlqnki
      781 ldsfidsmmd pheaahvlra yctlrlpyke lagvgidltv epffralvra vnkkvlkelr
      841 tkarilvppn ygrtmfgvld etgtleygqv fvqysndmlr ykvndpatil egdvivtknp
      901 cmhpgdirkl eavnvpqlhh vrdcivfpqk gerphpdema gsdldgdeys vlwyedlifn
      961 nncnpmhyys dppkerkasi gvqdmvdffc qyikgdkigl ianahlvwad ildgginshr
     1021 crelarkcav nldfakcgdl kglqnsekpp mypdfmekld tkntycsrkv lgqlyrnckk
     1081 velsteclev veeslpdprl llegreqfmk eatstykrya kkirallksy rieseseals
     1141 gavsklskym kendstdmam vlesqvehvv rqtreeffse qldeahenlk asawyqvtye
     1201 lqsseggiqs fpwvvsdvlm rivvntsscl pvpasrnsfs qrlgalllgv phpgggdqdg
     1261 aqhgdtqvlt nllrlmydwi dssreflfik daeelglykn imreacvkvs rslsrdmpph
     1321 klvilclrfa cawclkifqg gsdgeviske crrryrlghl alitlnrlsm sgnlaylcra
     1381 pegcpsteli riyikredev lfeilrryed ivteimmdws gvediqcdlk mdrmdewflq
     1441 lmvtgsrwal erlkeivvyp sfrevlllaf erkknaiagt le
//
LOCUS       XP_077501559            1482 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112633 [Amblyomma americanum].
ACCESSION   XP_077501559
VERSION     XP_077501559.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645433.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1482
                     /product="uncharacterized protein LOC144112633"
                     /calculated_mol_wt=167279
     Region          518..1078
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1482
                     /gene="LOC144112633"
                     /coded_by="XM_077645433.1:1127..5575"
                     /db_xref="GeneID:144112633"
ORIGIN      
        1 meppakpllp gpmpltsasc ardrqdncre malrvhclds gwgtptasql rrllglqcav
       61 gqvvagdcge vevyespnwt anvtlklers pkdsfmhivl kwcdqyqngs nngaaagksg
      121 ppewwlasee leefcdpyst qiatehkdvp vnafalgcfl gyntfvahfd stytmpppps
      181 plsstkssss hsgksmsvkr gkkgrgakss cpsdgtsssa ktacpnselt spegfkvtqc
      241 nahfehcfng lylyfvdvrk rsstrfrllv rysticriil rktsptctiv ylqllhppll
      301 ykvcagnsat sqcncegdyl pqfdlsdttp wdravefgeg pfkcgadvlg rsrvmrltls
      361 gprhwaplcn lilrtrknvq vfyapvrelq arelkavarp svqdfgvmya llsvwsesfq
      421 vtdelamdaq scretllela ahaeecpral eealfeiyfs lsqgkvfwfr salehlyfry
      481 rahrqcidvf tgsviaqelp shmvkvrkvi ltptrrvymp pqlvcksrll qhcdpdyalr
      541 ivvrdddckl isfslgackd dflsavikpp llsglkiggr rfmflgcsts qlrnhgiwfy
      601 ahdnqgrtae qirdgigdls tipsvpkfma rlgqafsqsl glltvprqnt svepdichaa
      661 pgrssdgshr eyifsdgigr ishgllrkvh haleleegee pcaiqirygg ckgmllldpt
      721 lsgcrivfre smrkfhsdhs dlyvlktskp rvlylnrpmi tileqsgika evflmlqnki
      781 ldsfidsmmd pheaahvlra yctlrlpyke lagvgidltv epffralvra vnkkvlkelr
      841 tkarilvppn ygrtmfgvld etgtleygqv fvqysndmlr ykvndpatil egdvivtknp
      901 cmhpgdirkl eavnvpqlhh vrdcivfpqk gerphpdema gsdldgdeys vlwyedlifn
      961 nncnpmhyys dppkerkasi gvqdmvdffc qyikgdkigl ianahlvwad ildgginshr
     1021 crelarkcav nldfakcgdl kglqnsekpp mypdfmekld tkntycsrkv lgqlyrnckk
     1081 velsteclev veeslpdprl llegreqfmk eatstykrya kkirallksy rieseseals
     1141 gavsklskym kendstdmam vlesqvehvv rqtreeffse qldeahenlk asawyqvtye
     1201 lqsseggiqs fpwvvsdvlm rivvntsscl pvpasrnsfs qrlgalllgv phpgggdqdg
     1261 aqhgdtqvlt nllrlmydwi dssreflfik daeelglykn imreacvkvs rslsrdmpph
     1321 klvilclrfa cawclkifqg gsdgeviske crrryrlghl alitlnrlsm sgnlaylcra
     1381 pegcpsteli riyikredev lfeilrryed ivteimmdws gvediqcdlk mdrmdewflq
     1441 lmvtgsrwal erlkeivvyp sfrevlllaf erkknaiagt le
//
LOCUS       XP_077501560             213 aa            linear   INV 25-NOV-2025
DEFINITION  inactive selenide, water dikinase-like protein [Amblyomma
            americanum].
ACCESSION   XP_077501560
VERSION     XP_077501560.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645434.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..213
                     /product="inactive selenide, water dikinase-like protein"
                     /calculated_mol_wt=23459
     Region          <17..182
                     /region_name="PurM-like"
                     /note="AIR (aminoimidazole ribonucleotide) synthase
                     related protein. This family includes Hydrogen
                     expression/formation protein HypE, AIR synthases, FGAM
                     (formylglycinamidine ribonucleotide) synthase and
                     Selenophosphate synthetase (SelD). The N-terminal
                     domain...; cl10019"
                     /db_xref="CDD:471963"
     CDS             1..213
                     /gene="LOC144112634"
                     /coded_by="XM_077645434.1:483..1124"
                     /db_xref="GeneID:144112634"
ORIGIN      
        1 mllassvlkv kkrpsdkrpd naqpgdvlvl tkplgtqvav nahqwlekpe rraqldkagi
       61 seedvrrvyv ramdcmarln lnarlmhkhg chgatdvtgf sllghaqnlv qvqqrsvgfv
      121 ihtlpiiakv pallpatgts fklmhghsae tscgllmclp kenadaycke ieqqegdpaw
      181 iiedvvegew varlmdsprm levpsadkes klw
//
LOCUS       XP_077501561             166 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112636 [Amblyomma americanum].
ACCESSION   XP_077501561
VERSION     XP_077501561.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645435.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..166
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..166
                     /product="uncharacterized protein LOC144112636"
                     /calculated_mol_wt=18557
     CDS             1..166
                     /gene="LOC144112636"
                     /coded_by="XM_077645435.1:472..972"
                     /db_xref="GeneID:144112636"
ORIGIN      
        1 mqaavdgpfs llmcitmmrw pfpgsllqam vvlilaqaah arcsvlpptl rllplhishp
       61 ewlflrahsg tssahgalew lrwsqtaert teflwqcalp qlraitkkth qesfspvhty
      121 gevslpeqvq dvlrrgpkfa veprrsapel lametcrqpl mglshc
//
LOCUS       XP_077501562            1116 aa            linear   INV 25-NOV-2025
DEFINITION  eukaryotic translation initiation factor 3 subunit a isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077501562
VERSION     XP_077501562.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645436.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1116
                     /product="eukaryotic translation initiation factor 3
                     subunit a isoform X1"
                     /calculated_mol_wt=133711
     Region          406..494
                     /region_name="PCI"
                     /note="PCI domain; pfam01399"
                     /db_xref="CDD:460195"
     Region          <538..932
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          867..>1106
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     CDS             1..1116
                     /gene="eIF3a"
                     /coded_by="XM_077645436.1:130..3480"
                     /db_xref="GeneID:144112637"
ORIGIN      
        1 mptyfqrpen alkrarefid vgkkhralda lydviksrkh rtwqkvhepi mqqylelcve
       61 lkkshiakeg lfqyrnicqq vniksledvv rgyldlaeek teaareesqq avvdiddldn
      121 vqtpesllls avssedtqdr tdrvvltpwv kflwesyrqc lellrnnsrv eclyhdiarq
      181 afkfclkynr ktefrklcdh lrahlgqihr hqsqqtsvnl nnpesqamhl etrlvqldsa
      241 iqmelwqeay katedihglm tlskkspkpq lmanyyqkla lvfwkagnhl fhasalfryf
      301 hlakdlkkni tqeeiqrmas rvvlatlavp mppnrpeidr lveteenvge knqrllatll
      361 glnnpptras lvkelvrlgv vglappqlqd lyrwlevdfq plklcgrvtq cfdfinkwee
      421 cselrmyvsa lqditvmrll kevsqvyqti eikrllelap fasafylenm vvdavrrndl
      481 qvridhkrrc lhfgselsvs qqeevvegph lqgmpseqir cqlvklysvl qrsvhmirpe
      541 eikdstkdlk mhiieayrlv ckkehkkile rqqiiearke mlenltfqre eeerkqmeer
      601 qqkmreaeee rivreaeera kqrsqreqde mkkkvvleki ellrktdiga rifegleeee
      661 lerldpeeil nrqveqldre rkemqtklkk qerkvdymer akrieeipll qeayerfkle
      721 dqkfweqkee erirllvqer evalqhktrl lrmrddkeef lskltqdrys vykekrqqfe
      781 qrleeerrkr laarkaerke errekflrer eetrrraale eqkrieaerl aklremeaik
      841 larereieek qrrmeeeerq rvearqppvr seesswrkre edpdrknepw rsrvrdnres
      901 errsdgieke qsygrrdrdr dnedswrrrk dddgegeprs rpsdiswrka epdvdrpssg
      961 rsggglwrsg dnrirdsdrr lrdyddrgrr eygddrrppp rdfdrderrp paprdsdred
     1021 rgrqdfgrdf gddsrrrrdm grgrdfgddr psrageadnw rsglkesrgg gpawqsddkr
     1081 psprkddrit gswrekedrp nkpredaded gwktvr
//
LOCUS       XP_077501563            1113 aa            linear   INV 25-NOV-2025
DEFINITION  eukaryotic translation initiation factor 3 subunit a isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077501563
VERSION     XP_077501563.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645437.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1113
                     /product="eukaryotic translation initiation factor 3
                     subunit a isoform X2"
                     /calculated_mol_wt=133467
     Region          406..494
                     /region_name="PCI"
                     /note="PCI domain; pfam01399"
                     /db_xref="CDD:460195"
     Region          <566..932
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          867..>1103
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     CDS             1..1113
                     /gene="eIF3a"
                     /coded_by="XM_077645437.1:130..3471"
                     /db_xref="GeneID:144112637"
ORIGIN      
        1 mptyfqrpen alkrarefid vgkkhralda lydviksrkh rtwqkvhepi mqqylelcve
       61 lkkshiakeg lfqyrnicqq vniksledvv rgyldlaeek teaareesqq avvdiddldn
      121 vqtpesllls avssedtqdr tdrvvltpwv kflwesyrqc lellrnnsrv eclyhdiarq
      181 afkfclkynr ktefrklcdh lrahlgqihr hqsqqtsvnl nnpesqamhl etrlvqldsa
      241 iqmelwqeay katedihglm tlskkspkpq lmanyyqkla lvfwkagnhl fhasalfryf
      301 hlakdlkkni tqeeiqrmas rvvlatlavp mppnrpeidr lveteenvge knqrllatll
      361 glnnpptras lvkelvrlgv vglappqlqd lyrwlevdfq plklcgrvtq cfdfinkwee
      421 cselrmyvsa lqditvmrll kevsqvyqti eikrllelap fasafylenm vvdavrrndl
      481 qvridhkrrc lhfgselsvs qqeevvegph lqgmpseqir cqlvklysvl qrsvhmirpe
      541 eikdstkdlk mhiieayrlv ckkehkkile rqqiiearke mlenltfqre eeerkqmeer
      601 qqkmreaeee rivreaeera kqrsqreqde mkkkvvleki ellrktdiga rifegleeee
      661 lerldpeeil nrqveqldre rkemqtklkk qerkvdymer akrieeipll qeayerfkle
      721 dqkfweqkee erirllvqer evalqhktrl lrmrddkeef lskltqdrys vykekrqqfe
      781 qrleeerrkr laarkaerke errekflrer eetrrraale eqkrieaerl aklremeaik
      841 larereieek qrrmeeeerq rvearqppvr seesswrkre edpdrknepw rsrvrdnres
      901 errsdgieke qsygrrdrdr dnedswrrrk dddeprsrps diswrkaepd vdrpssgrsg
      961 gglwrsgdnr irdsdrrlrd yddrgrreyg ddrrppprdf drderrppap rdsdredrgr
     1021 qdfgrdfgdd srrrrdmgrg rdfgddrpsr ageadnwrsg lkesrgggpa wqsddkrpsp
     1081 rkddritgsw rekedrpnkp redadedgwk tvr
//
LOCUS       XP_077501564             388 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112638 [Amblyomma americanum].
ACCESSION   XP_077501564
VERSION     XP_077501564.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645438.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..388
                     /product="uncharacterized protein LOC144112638"
                     /calculated_mol_wt=43360
     Region          9..51
                     /region_name="PHD_SF"
                     /note="PHD finger superfamily; cl22851"
                     /db_xref="CDD:473978"
     Site            order(16..19,23,44)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:276966"
     Region          204..357
                     /region_name="TesA"
                     /note="Lysophospholipase L1 or related esterase. Includes
                     spore coat protein LipC/YcsK [Cell cycle control, cell
                     division, chromosome partitioning, Lipid transport and
                     metabolism]; COG2755"
                     /db_xref="CDD:442045"
     CDS             1..388
                     /gene="LOC144112638"
                     /coded_by="XM_077645438.1:145..1311"
                     /db_xref="GeneID:144112638"
ORIGIN      
        1 mgrqarkvke dedgelvqcq ecdrwcylde tgfgslaead easfvckmck rfgdavhalk
       61 geweagfnal nadwqverea rikleaqvad likkeennaa llkrmvgeik eerekriqle
      121 kqvealrsrp aghpssekcq agdearqsys avvqrassak nenamkkvka gmetrdnsvq
      181 rqerqlersg gqqmelgser lgrrrvlvvg dsnvarvegg vltavkadrr vqveaqsgkc
      241 mvdamarare vvvgsmdgeh lvvihaglnd vlqgrsqnle tqlevgmrrl reasesvhvt
      301 ictvpevqrq areterrvve tnrvirllsr rlryevmevn revyevrphp faqdgihygg
      361 atgkkvgsri grqataflgg pralmepv
//
LOCUS       XP_077501565             769 aa            linear   INV 25-NOV-2025
DEFINITION  adducin 1-like protein hts isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501565
VERSION     XP_077501565.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645439.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..769
                     /product="adducin 1-like protein hts isoform X1"
                     /calculated_mol_wt=86308
     Region          120..342
                     /region_name="Aldolase_II"
                     /note="Class II Aldolase and Adducin head (N-terminal)
                     domain. Aldolases are ubiquitous enzymes catalyzing
                     central steps of carbohydrate metabolism. Based on
                     enzymatic mechanisms, this superfamily has been divided
                     into two distinct classes (Class I and II); cl00214"
                     /db_xref="CDD:469663"
     Site            order(134,138,146,171,173..174,224,226..227,232..234,242,
                     244..245,256,281,292,299,303,310)
                     /site_type="other"
                     /note="intersubunit interface [polypeptide binding]"
                     /db_xref="CDD:238232"
     Site            order(151,168..169,194..196,222,224,282)
                     /site_type="active"
                     /db_xref="CDD:238232"
     Site            order(222,224,282)
                     /site_type="other"
                     /note="Zn2+ binding site [ion binding]"
                     /db_xref="CDD:238232"
     CDS             1..769
                     /gene="hts"
                     /coded_by="XM_077645439.1:150..2459"
                     /db_xref="GeneID:144112639"
ORIGIN      
        1 maetkaqtng lsdgqakaer ydpddpeaqr qmwrppdieq dmkemerrkr vemimnsqlf
       61 reeleriies qlsegytpsn maalqqvtel llphatkqst irggrclvpi ndvrgidglr
      121 yskgekllrc klaslcrlld aygwttaafn hitlrvsqdq ehflvnpfgl lyhevtassl
      181 lkldmqsnvi dsgtttfhfn rpaymihaai hanrpdikci ihlqhppcva vsslkqgllp
      241 itrdaallge iayheykgss adtheiarsl gpnskvlflr nhgvlvcgds vedafsnlcs
      301 tieacetqmk lapigfdnvh liseeaqrsm reeharlkga depvpggded kekaepkekp
      361 krwrpcdivf eahmrtldns ghrtgyiyrq plmrneqprl ktdvevppsa ssiaqfleed
      421 kwlsplkkll dgrktqdklr wvnspntyqr vevletgtad pkkitkwvqe gspshstsvk
      481 idtshqfvpk gtdpaefkrk qkelkenrln skinagpqsn lldgvtweev kkmqdavasa
      541 tgdqvvlvga askgiiqrdy qhnamvykta ysknpfdnit dseledykrl verkqrgesi
      601 dddvpdslkp llvepvavps qgpqhtsspl qspaqsptsp asdeedskss arvkrslssr
      661 lateeeeriv pqqfgrraqs erkpkrqsda pvngdeklrs aesgdvsaas rsskeqsspv
      721 kdvegespkk dkkkkkgglr tpsflkkkkh rkekedkeka asewqnrsq
//
LOCUS       XP_077501566             768 aa            linear   INV 25-NOV-2025
DEFINITION  adducin 1-like protein hts isoform X2 [Amblyomma americanum].
ACCESSION   XP_077501566
VERSION     XP_077501566.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645440.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..768
                     /product="adducin 1-like protein hts isoform X2"
                     /calculated_mol_wt=86179
     Region          120..342
                     /region_name="Aldolase_II"
                     /note="Class II Aldolase and Adducin head (N-terminal)
                     domain. Aldolases are ubiquitous enzymes catalyzing
                     central steps of carbohydrate metabolism. Based on
                     enzymatic mechanisms, this superfamily has been divided
                     into two distinct classes (Class I and II); cl00214"
                     /db_xref="CDD:469663"
     Site            order(134,138,146,171,173..174,224,226..227,232..234,242,
                     244..245,256,281,292,299,303,310)
                     /site_type="other"
                     /note="intersubunit interface [polypeptide binding]"
                     /db_xref="CDD:238232"
     Site            order(151,168..169,194..196,222,224,282)
                     /site_type="active"
                     /db_xref="CDD:238232"
     Site            order(222,224,282)
                     /site_type="other"
                     /note="Zn2+ binding site [ion binding]"
                     /db_xref="CDD:238232"
     CDS             1..768
                     /gene="hts"
                     /coded_by="XM_077645440.1:150..2456"
                     /db_xref="GeneID:144112639"
ORIGIN      
        1 maetkaqtng lsdgqakaer ydpddpeaqr qmwrppdieq dmkemerrkr vemimnsqlf
       61 reeleriies qlsegytpsn maalqqvtel llphatkqst irggrclvpi ndvrgidglr
      121 yskgekllrc klaslcrlld aygwttaafn hitlrvsqdq ehflvnpfgl lyhevtassl
      181 lkldmqsnvi dsgtttfhfn rpaymihaai hanrpdikci ihlqhppcva vsslkqgllp
      241 itrdaallge iayheykgss adtheiarsl gpnskvlflr nhgvlvcgds vedafsnlcs
      301 tieacetqmk lapigfdnvh liseeaqrsm reeharlkga depvpggded kekaepkekp
      361 krwrpcdivf eahmrtldns ghrtgyiyrq plmrneqprl ktdvevppsa ssiaqfleed
      421 kwlsplkkll dgrktqdklr wvnspntyqr vevletgtad pkkitkwvqe gspshstsvk
      481 idtshqfvpk gtdpaefkrk qkelkenrln skinagpqsn lldgvtweev kkmqdavasa
      541 tgdqvvlvga askgiiqrdy qhnamvykta ysknpfdnit dseledykrl verkqrgesi
      601 dddvpdslkp llvepvavps qgpqhtsspl qspaqsptsp asdeedskss arvkrslssr
      661 lateeeeriv pqqfgrraqs erkpkrqsda pvngdeklrs aesgdvsaas rsskesspvk
      721 dvegespkkd kkkkkgglrt psflkkkkhr kekedkekaa sewqnrsq
//
LOCUS       XP_077501567             752 aa            linear   INV 25-NOV-2025
DEFINITION  adducin 1-like protein hts isoform X3 [Amblyomma americanum].
ACCESSION   XP_077501567
VERSION     XP_077501567.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645441.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..752
                     /product="adducin 1-like protein hts isoform X3"
                     /calculated_mol_wt=84165
     Region          120..342
                     /region_name="Aldolase_II"
                     /note="Class II Aldolase and Adducin head (N-terminal)
                     domain. Aldolases are ubiquitous enzymes catalyzing
                     central steps of carbohydrate metabolism. Based on
                     enzymatic mechanisms, this superfamily has been divided
                     into two distinct classes (Class I and II); cl00214"
                     /db_xref="CDD:469663"
     Site            order(134,138,146,171,173..174,224,226..227,232..234,242,
                     244..245,256,281,292,299,303,310)
                     /site_type="other"
                     /note="intersubunit interface [polypeptide binding]"
                     /db_xref="CDD:238232"
     Site            order(151,168..169,194..196,222,224,282)
                     /site_type="active"
                     /db_xref="CDD:238232"
     Site            order(222,224,282)
                     /site_type="other"
                     /note="Zn2+ binding site [ion binding]"
                     /db_xref="CDD:238232"
     CDS             1..752
                     /gene="hts"
                     /coded_by="XM_077645441.1:150..2408"
                     /db_xref="GeneID:144112639"
ORIGIN      
        1 maetkaqtng lsdgqakaer ydpddpeaqr qmwrppdieq dmkemerrkr vemimnsqlf
       61 reeleriies qlsegytpsn maalqqvtel llphatkqst irggrclvpi ndvrgidglr
      121 yskgekllrc klaslcrlld aygwttaafn hitlrvsqdq ehflvnpfgl lyhevtassl
      181 lkldmqsnvi dsgtttfhfn rpaymihaai hanrpdikci ihlqhppcva vsslkqgllp
      241 itrdaallge iayheykgss adtheiarsl gpnskvlflr nhgvlvcgds vedafsnlcs
      301 tieacetqmk lapigfdnvh liseeaqrsm reeharlkga depvpggded kekaepkekp
      361 krwrpcdivf eahmrtldns ghrtgyiyrq plmrneqprl ktdvevppsa ssiaqfleed
      421 kwlsplkkll dgrktqdklr wvnspntyqr vevletgtad pkkitkwvqe gspshstsvk
      481 idtshqfvpk gtdpaefkrk qkelkenrln skinagpqsn lldgvtweev kkmqdavasa
      541 tgdqvvlvga askgiiqrdy qhnamvykta ysknpfdnit dseledykrl verkqrgesi
      601 dddvpdslkp llvepvavps qgpqhtsspl qspaqsptsp asdeevktav vtavrylqpn
      661 mtepqiiitd gdapvngdek lrsaesgdvs aasrsskeqs spvkdveges pkkdkkkkkg
      721 glrtpsflkk kkhrkekedk ekaasewqnr sq
//
LOCUS       XP_077501568             751 aa            linear   INV 25-NOV-2025
DEFINITION  adducin 1-like protein hts isoform X4 [Amblyomma americanum].
ACCESSION   XP_077501568
VERSION     XP_077501568.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645442.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..751
                     /product="adducin 1-like protein hts isoform X4"
                     /calculated_mol_wt=84037
     Region          120..342
                     /region_name="Aldolase_II"
                     /note="Class II Aldolase and Adducin head (N-terminal)
                     domain. Aldolases are ubiquitous enzymes catalyzing
                     central steps of carbohydrate metabolism. Based on
                     enzymatic mechanisms, this superfamily has been divided
                     into two distinct classes (Class I and II); cl00214"
                     /db_xref="CDD:469663"
     Site            order(134,138,146,171,173..174,224,226..227,232..234,242,
                     244..245,256,281,292,299,303,310)
                     /site_type="other"
                     /note="intersubunit interface [polypeptide binding]"
                     /db_xref="CDD:238232"
     Site            order(151,168..169,194..196,222,224,282)
                     /site_type="active"
                     /db_xref="CDD:238232"
     Site            order(222,224,282)
                     /site_type="other"
                     /note="Zn2+ binding site [ion binding]"
                     /db_xref="CDD:238232"
     CDS             1..751
                     /gene="hts"
                     /coded_by="XM_077645442.1:150..2405"
                     /db_xref="GeneID:144112639"
ORIGIN      
        1 maetkaqtng lsdgqakaer ydpddpeaqr qmwrppdieq dmkemerrkr vemimnsqlf
       61 reeleriies qlsegytpsn maalqqvtel llphatkqst irggrclvpi ndvrgidglr
      121 yskgekllrc klaslcrlld aygwttaafn hitlrvsqdq ehflvnpfgl lyhevtassl
      181 lkldmqsnvi dsgtttfhfn rpaymihaai hanrpdikci ihlqhppcva vsslkqgllp
      241 itrdaallge iayheykgss adtheiarsl gpnskvlflr nhgvlvcgds vedafsnlcs
      301 tieacetqmk lapigfdnvh liseeaqrsm reeharlkga depvpggded kekaepkekp
      361 krwrpcdivf eahmrtldns ghrtgyiyrq plmrneqprl ktdvevppsa ssiaqfleed
      421 kwlsplkkll dgrktqdklr wvnspntyqr vevletgtad pkkitkwvqe gspshstsvk
      481 idtshqfvpk gtdpaefkrk qkelkenrln skinagpqsn lldgvtweev kkmqdavasa
      541 tgdqvvlvga askgiiqrdy qhnamvykta ysknpfdnit dseledykrl verkqrgesi
      601 dddvpdslkp llvepvavps qgpqhtsspl qspaqsptsp asdeevktav vtavrylqpn
      661 mtepqiiitd gdapvngdek lrsaesgdvs aasrsskess pvkdvegesp kkdkkkkkgg
      721 lrtpsflkkk khrkekedke kaasewqnrs q
//
LOCUS       XP_077501569             746 aa            linear   INV 25-NOV-2025
DEFINITION  adducin 1-like protein hts isoform X5 [Amblyomma americanum].
ACCESSION   XP_077501569
VERSION     XP_077501569.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645443.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..746
                     /product="adducin 1-like protein hts isoform X5"
                     /calculated_mol_wt=83442
     Region          120..342
                     /region_name="Aldolase_II"
                     /note="Class II Aldolase and Adducin head (N-terminal)
                     domain. Aldolases are ubiquitous enzymes catalyzing
                     central steps of carbohydrate metabolism. Based on
                     enzymatic mechanisms, this superfamily has been divided
                     into two distinct classes (Class I and II); cl00214"
                     /db_xref="CDD:469663"
     Site            order(134,138,146,171,173..174,224,226..227,232..234,242,
                     244..245,256,281,292,299,303,310)
                     /site_type="other"
                     /note="intersubunit interface [polypeptide binding]"
                     /db_xref="CDD:238232"
     Site            order(151,168..169,194..196,222,224,282)
                     /site_type="active"
                     /db_xref="CDD:238232"
     Site            order(222,224,282)
                     /site_type="other"
                     /note="Zn2+ binding site [ion binding]"
                     /db_xref="CDD:238232"
     CDS             1..746
                     /gene="hts"
                     /coded_by="XM_077645443.1:150..2390"
                     /db_xref="GeneID:144112639"
ORIGIN      
        1 maetkaqtng lsdgqakaer ydpddpeaqr qmwrppdieq dmkemerrkr vemimnsqlf
       61 reeleriies qlsegytpsn maalqqvtel llphatkqst irggrclvpi ndvrgidglr
      121 yskgekllrc klaslcrlld aygwttaafn hitlrvsqdq ehflvnpfgl lyhevtassl
      181 lkldmqsnvi dsgtttfhfn rpaymihaai hanrpdikci ihlqhppcva vsslkqgllp
      241 itrdaallge iayheykgss adtheiarsl gpnskvlflr nhgvlvcgds vedafsnlcs
      301 tieacetqmk lapigfdnvh liseeaqrsm reeharlkga depvpggded kekaepkekp
      361 krwrpcdivf eahmrtldns ghrtgyiyrq plmrneqprl ktdvevppsa ssiaqfleed
      421 kwlsplkkll dgrktqdklr wvnspntyqr vevletgtad pkkitkwvqe gspshstsvk
      481 idtshqfvpk gtdpaefkrk qkelkenrln skinagpqsn lldgvtweev kkmqdavasa
      541 tgdqvvlvga askgiiqrdy qhnamvykta ysknpfdnit dseledykrl verkqrgesi
      601 dddvpdslkp llvepvavps qgpqhtsspl qspaqsptsp asdeedskss arvkrslssr
      661 lategdapvn gdeklrsaes gdvsaasrss keqsspvkdv egespkkdkk kkkgglrtps
      721 flkkkkhrke kedkekaase wqnrsq
//
LOCUS       XP_077501570             745 aa            linear   INV 25-NOV-2025
DEFINITION  adducin 1-like protein hts isoform X6 [Amblyomma americanum].
ACCESSION   XP_077501570
VERSION     XP_077501570.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645444.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..745
                     /product="adducin 1-like protein hts isoform X6"
                     /calculated_mol_wt=83314
     Region          120..342
                     /region_name="Aldolase_II"
                     /note="Class II Aldolase and Adducin head (N-terminal)
                     domain. Aldolases are ubiquitous enzymes catalyzing
                     central steps of carbohydrate metabolism. Based on
                     enzymatic mechanisms, this superfamily has been divided
                     into two distinct classes (Class I and II); cl00214"
                     /db_xref="CDD:469663"
     Site            order(134,138,146,171,173..174,224,226..227,232..234,242,
                     244..245,256,281,292,299,303,310)
                     /site_type="other"
                     /note="intersubunit interface [polypeptide binding]"
                     /db_xref="CDD:238232"
     Site            order(151,168..169,194..196,222,224,282)
                     /site_type="active"
                     /db_xref="CDD:238232"
     Site            order(222,224,282)
                     /site_type="other"
                     /note="Zn2+ binding site [ion binding]"
                     /db_xref="CDD:238232"
     CDS             1..745
                     /gene="hts"
                     /coded_by="XM_077645444.1:150..2387"
                     /db_xref="GeneID:144112639"
ORIGIN      
        1 maetkaqtng lsdgqakaer ydpddpeaqr qmwrppdieq dmkemerrkr vemimnsqlf
       61 reeleriies qlsegytpsn maalqqvtel llphatkqst irggrclvpi ndvrgidglr
      121 yskgekllrc klaslcrlld aygwttaafn hitlrvsqdq ehflvnpfgl lyhevtassl
      181 lkldmqsnvi dsgtttfhfn rpaymihaai hanrpdikci ihlqhppcva vsslkqgllp
      241 itrdaallge iayheykgss adtheiarsl gpnskvlflr nhgvlvcgds vedafsnlcs
      301 tieacetqmk lapigfdnvh liseeaqrsm reeharlkga depvpggded kekaepkekp
      361 krwrpcdivf eahmrtldns ghrtgyiyrq plmrneqprl ktdvevppsa ssiaqfleed
      421 kwlsplkkll dgrktqdklr wvnspntyqr vevletgtad pkkitkwvqe gspshstsvk
      481 idtshqfvpk gtdpaefkrk qkelkenrln skinagpqsn lldgvtweev kkmqdavasa
      541 tgdqvvlvga askgiiqrdy qhnamvykta ysknpfdnit dseledykrl verkqrgesi
      601 dddvpdslkp llvepvavps qgpqhtsspl qspaqsptsp asdeedskss arvkrslssr
      661 lategdapvn gdeklrsaes gdvsaasrss kesspvkdve gespkkdkkk kkgglrtpsf
      721 lkkkkhrkek edkekaasew qnrsq
//
LOCUS       XP_077501571             467 aa            linear   INV 25-NOV-2025
DEFINITION  26S proteasome non-ATPase regulatory subunit 4-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077501571
VERSION     XP_077501571.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645445.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..467
                     /product="26S proteasome non-ATPase regulatory subunit
                     4-like isoform X1"
                     /calculated_mol_wt=50415
     Region          1..171
                     /region_name="vWFA"
                     /note="Von Willebrand factor type A (vWA) domain was
                     originally found in the blood coagulation protein von
                     Willebrand factor (vWF). Typically, the vWA domain is made
                     up of approximately 200 amino acid residues folded into a
                     classic a/b para-rossmann type of...; cl00057"
                     /db_xref="CDD:469594"
     Region          354..>445
                     /region_name="PRK11855"
                     /note="dihydrolipoamide acetyltransferase; Reviewed"
                     /db_xref="CDD:237000"
     CDS             1..467
                     /gene="LOC144112640"
                     /coded_by="XM_077645445.1:20..1423"
                     /db_xref="GeneID:144112640"
ORIGIN      
        1 mrngdfeptr ldaqyeavkf ifraktrshr ennvalltls rpkvlatltt darhflskle
       61 emkpdgcidf vkalriaklv lqnrldkvhq mrivafvgsp vtsdkkells laiklkrenv
      121 kvdivsfgen dlniptltpf lsiligggya gshmvsveag nvlwrvlmns pivraqddtp
      181 ftyrinsldf dledddpela lvlrvsmeeh rqiearrama naangaalap ssdgagampa
      241 eeellehmss gtgqggaftg mqhyeatted ddlavaveml lslgsaevpa qylpdavaae
      301 eepletnesk eppkkaeaaa aakydsafed paffssfles lpdpgsesda acnavgdtil
      361 edqnkaaevp aqylpdavaa eeepvetnes keppkkaept aaaakydsaf qdpaffsslp
      421 eslpdpgses daacnavgdt iledqnkgad tgedentpdk dkltkre
//
LOCUS       XP_077501572             498 aa            linear   INV 25-NOV-2025
DEFINITION  26S proteasome non-ATPase regulatory subunit 4-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077501572
VERSION     XP_077501572.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645446.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..498
                     /product="26S proteasome non-ATPase regulatory subunit
                     4-like isoform X2"
                     /calculated_mol_wt=53637
     Region          21..198
                     /region_name="vWFA"
                     /note="Von Willebrand factor type A (vWA) domain was
                     originally found in the blood coagulation protein von
                     Willebrand factor (vWF). Typically, the vWA domain is made
                     up of approximately 200 amino acid residues folded into a
                     classic a/b para-rossmann type of...; cl00057"
                     /db_xref="CDD:469594"
     Site            order(23,95,125)
                     /site_type="other"
                     /note="metal ion-dependent adhesion site (MIDAS)"
                     /db_xref="CDD:238119"
     Region          382..>476
                     /region_name="PRK11855"
                     /note="dihydrolipoamide acetyltransferase; Reviewed"
                     /db_xref="CDD:237000"
     CDS             1..498
                     /gene="LOC144112640"
                     /coded_by="XM_077645446.1:16..1512"
                     /db_xref="GeneID:144112640"
ORIGIN      
        1 mpadnkelpl avsipdaacr cvdtsdymrn gdfeptrlda qyeavkfifr aktrshrenn
       61 valltlsrpk vlatlttdar hflskleemk pdgcidfvka lriaklvlqn rldkvhqmri
      121 vafvgspvts dkkellslai klkrenvkvd ivsfgendln iptltpflsi ligggyagsh
      181 mvsveagnvl wrvlmnspiv raqddtpfty rinsldfdle dddpelalvl rvsmeehrqi
      241 earramanaa ngaalapssd gagampaeee llehmssgtg qggaftgmqh yeattedddl
      301 avavemllsl gsaevpaqyl pdavaaeeep letneskepp kkaeaaaaak ydsafedpaf
      361 fssfleslpd pgsesdaacn avgdtiledq nkgadtaaev paqylpdava aeeepvetne
      421 skeppkkaep taaaakydsa fqdpaffssl peslpdpgse sdaacnavgd tiledqnkga
      481 dtgedentpd kdkltkre
//
LOCUS       XP_077501573             493 aa            linear   INV 25-NOV-2025
DEFINITION  26S proteasome non-ATPase regulatory subunit 4-like isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077501573
VERSION     XP_077501573.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645447.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..493
                     /product="26S proteasome non-ATPase regulatory subunit
                     4-like isoform X3"
                     /calculated_mol_wt=53282
     Region          17..193
                     /region_name="vWFA"
                     /note="Von Willebrand factor type A (vWA) domain was
                     originally found in the blood coagulation protein von
                     Willebrand factor (vWF). Typically, the vWA domain is made
                     up of approximately 200 amino acid residues folded into a
                     classic a/b para-rossmann type of...; cl00057"
                     /db_xref="CDD:469594"
     Site            order(18,90,120)
                     /site_type="other"
                     /note="metal ion-dependent adhesion site (MIDAS)"
                     /db_xref="CDD:238119"
     Region          377..>471
                     /region_name="PRK11855"
                     /note="dihydrolipoamide acetyltransferase; Reviewed"
                     /db_xref="CDD:237000"
     CDS             1..493
                     /gene="LOC144112640"
                     /coded_by="XM_077645447.1:16..1497"
                     /db_xref="GeneID:144112640"
ORIGIN      
        1 mflarivvlp tdiqrsvdts dymrngdfep trldaqyeav kfifraktrs hrennvallt
       61 lsrpkvlatl ttdarhflsk leemkpdgci dfvkalriak lvlqnrldkv hqmrivafvg
      121 spvtsdkkel lslaiklkre nvkvdivsfg endlniptlt pflsiliggg yagshmvsve
      181 agnvlwrvlm nspivraqdd tpftyrinsl dfdledddpe lalvlrvsme ehrqiearra
      241 manaangaal apssdgagam paeeellehm ssgtgqggaf tgmqhyeatt edddlavave
      301 mllslgsaev paqylpdava aeeepletne skeppkkaea aaaakydsaf edpaffssfl
      361 eslpdpgses daacnavgdt iledqnkgad taaevpaqyl pdavaaeeep vetneskepp
      421 kkaeptaaaa kydsafqdpa ffsslpeslp dpgsesdaac navgdtiled qnkgadtged
      481 entpdkdklt kre
//
LOCUS       XP_077501574             486 aa            linear   INV 25-NOV-2025
DEFINITION  26S proteasome non-ATPase regulatory subunit 4-like isoform X4
            [Amblyomma americanum].
ACCESSION   XP_077501574
VERSION     XP_077501574.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645448.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..486
                     /product="26S proteasome non-ATPase regulatory subunit
                     4-like isoform X4"
                     /calculated_mol_wt=52385
     Region          1..186
                     /region_name="vWFA"
                     /note="Von Willebrand factor type A (vWA) domain was
                     originally found in the blood coagulation protein von
                     Willebrand factor (vWF). Typically, the vWA domain is made
                     up of approximately 200 amino acid residues folded into a
                     classic a/b para-rossmann type of...; cl00057"
                     /db_xref="CDD:469594"
     Site            order(11,83,113)
                     /site_type="other"
                     /note="metal ion-dependent adhesion site (MIDAS)"
                     /db_xref="CDD:238119"
     Region          370..>464
                     /region_name="PRK11855"
                     /note="dihydrolipoamide acetyltransferase; Reviewed"
                     /db_xref="CDD:237000"
     CDS             1..486
                     /gene="LOC144112640"
                     /coded_by="XM_077645448.1:460..1920"
                     /db_xref="GeneID:144112640"
ORIGIN      
        1 mlpesavicv dtsdymrngd feptrldaqy eavkfifrak trshrennva lltlsrpkvl
       61 atlttdarhf lskleemkpd gcidfvkalr iaklvlqnrl dkvhqmriva fvgspvtsdk
      121 kellslaikl krenvkvdiv sfgendlnip tltpflsili gggyagshmv sveagnvlwr
      181 vlmnspivra qddtpftyri nsldfdledd dpelalvlrv smeehrqiea rramanaang
      241 aalapssdga gampaeeell ehmssgtgqg gaftgmqhye attedddlav avemllslgs
      301 aevpaqylpd avaaeeeple tneskeppkk aeaaaaakyd safedpaffs sfleslpdpg
      361 sesdaacnav gdtiledqnk gadtaaevpa qylpdavaae eepvetnesk eppkkaepta
      421 aaakydsafq dpaffsslpe slpdpgsesd aacnavgdti ledqnkgadt gedentpdkd
      481 kltkre
//
LOCUS       XP_077501575             464 aa            linear   INV 25-NOV-2025
DEFINITION  26S proteasome non-ATPase regulatory subunit 4-like isoform X5
            [Amblyomma americanum].
ACCESSION   XP_077501575
VERSION     XP_077501575.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645449.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..464
                     /product="26S proteasome non-ATPase regulatory subunit
                     4-like isoform X5"
                     /calculated_mol_wt=49633
     Region          <71..164
                     /region_name="vWFA"
                     /note="Von Willebrand factor type A (vWA) domain was
                     originally found in the blood coagulation protein von
                     Willebrand factor (vWF). Typically, the vWA domain is made
                     up of approximately 200 amino acid residues folded into a
                     classic a/b para-rossmann type of...; cl00057"
                     /db_xref="CDD:469594"
     Region          348..>442
                     /region_name="PRK11855"
                     /note="dihydrolipoamide acetyltransferase; Reviewed"
                     /db_xref="CDD:237000"
     CDS             1..464
                     /gene="LOC144112640"
                     /coded_by="XM_077645449.1:16..1410"
                     /db_xref="GeneID:144112640"
ORIGIN      
        1 mwcplsaldg dsgttspgar wfplrrhtlg ilqllllvlr rsdggtpvfa alrhgpqtam
       61 rypgrcqksf qlvlqnrldk vhqmrivafv gspvtsdkke llslaiklkr envkvdivsf
      121 gendlniptl tpflsiligg gyagshmvsv eagnvlwrvl mnspivraqd dtpftyrins
      181 ldfdledddp elalvlrvsm eehrqiearr amanaangaa lapssdgaga mpaeeelleh
      241 mssgtgqgga ftgmqhyeat tedddlavav emllslgsae vpaqylpdav aaeeepletn
      301 eskeppkkae aaaaakydsa fedpaffssf leslpdpgse sdaacnavgd tiledqnkga
      361 dtaaevpaqy lpdavaaeee pvetneskep pkkaeptaaa akydsafqdp affsslpesl
      421 pdpgsesdaa cnavgdtile dqnkgadtge dentpdkdkl tkre
//
LOCUS       XP_077501576             396 aa            linear   INV 25-NOV-2025
DEFINITION  26S proteasome non-ATPase regulatory subunit 4-like isoform X6
            [Amblyomma americanum].
ACCESSION   XP_077501576
VERSION     XP_077501576.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645450.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..396
                     /product="26S proteasome non-ATPase regulatory subunit
                     4-like isoform X6"
                     /calculated_mol_wt=42146
     Region          <4..96
                     /region_name="vWFA"
                     /note="Von Willebrand factor type A (vWA) domain was
                     originally found in the blood coagulation protein von
                     Willebrand factor (vWF). Typically, the vWA domain is made
                     up of approximately 200 amino acid residues folded into a
                     classic a/b para-rossmann type of...; cl00057"
                     /db_xref="CDD:469594"
     Region          280..>374
                     /region_name="PRK11855"
                     /note="dihydrolipoamide acetyltransferase; Reviewed"
                     /db_xref="CDD:237000"
     CDS             1..396
                     /gene="LOC144112640"
                     /coded_by="XM_077645450.1:16..1206"
                     /db_xref="GeneID:144112640"
ORIGIN      
        1 mvelvlqnrl dkvhqmriva fvgspvtsdk kellslaikl krenvkvdiv sfgendlnip
       61 tltpflsili gggyagshmv sveagnvlwr vlmnspivra qddtpftyri nsldfdledd
      121 dpelalvlrv smeehrqiea rramanaang aalapssdga gampaeeell ehmssgtgqg
      181 gaftgmqhye attedddlav avemllslgs aevpaqylpd avaaeeeple tneskeppkk
      241 aeaaaaakyd safedpaffs sfleslpdpg sesdaacnav gdtiledqnk gadtaaevpa
      301 qylpdavaae eepvetnesk eppkkaepta aaakydsafq dpaffsslpe slpdpgsesd
      361 aacnavgdti ledqnkgadt gedentpdkd kltkre
//
LOCUS       XP_077501577             394 aa            linear   INV 25-NOV-2025
DEFINITION  26S proteasome non-ATPase regulatory subunit 4-like isoform X7
            [Amblyomma americanum].
ACCESSION   XP_077501577
VERSION     XP_077501577.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645451.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..394
                     /product="26S proteasome non-ATPase regulatory subunit
                     4-like isoform X7"
                     /calculated_mol_wt=41917
     Region          <2..94
                     /region_name="vWFA"
                     /note="Von Willebrand factor type A (vWA) domain was
                     originally found in the blood coagulation protein von
                     Willebrand factor (vWF). Typically, the vWA domain is made
                     up of approximately 200 amino acid residues folded into a
                     classic a/b para-rossmann type of...; cl00057"
                     /db_xref="CDD:469594"
     Region          278..>372
                     /region_name="PRK11855"
                     /note="dihydrolipoamide acetyltransferase; Reviewed"
                     /db_xref="CDD:237000"
     CDS             1..394
                     /gene="LOC144112640"
                     /coded_by="XM_077645451.1:25..1209"
                     /db_xref="GeneID:144112640"
ORIGIN      
        1 mlvlqnrldk vhqmrivafv gspvtsdkke llslaiklkr envkvdivsf gendlniptl
       61 tpflsiligg gyagshmvsv eagnvlwrvl mnspivraqd dtpftyrins ldfdledddp
      121 elalvlrvsm eehrqiearr amanaangaa lapssdgaga mpaeeelleh mssgtgqgga
      181 ftgmqhyeat tedddlavav emllslgsae vpaqylpdav aaeeepletn eskeppkkae
      241 aaaaakydsa fedpaffssf leslpdpgse sdaacnavgd tiledqnkga dtaaevpaqy
      301 lpdavaaeee pvetneskep pkkaeptaaa akydsafqdp affsslpesl pdpgsesdaa
      361 cnavgdtile dqnkgadtge dentpdkdkl tkre
//
LOCUS       XP_077501578             401 aa            linear   INV 25-NOV-2025
DEFINITION  26S proteasome non-ATPase regulatory subunit 4-like [Amblyomma
            americanum].
ACCESSION   XP_077501578
VERSION     XP_077501578.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645452.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..401
                     /product="26S proteasome non-ATPase regulatory subunit
                     4-like"
                     /calculated_mol_wt=43989
     Region          6..186
                     /region_name="vWFA"
                     /note="Von Willebrand factor type A (vWA) domain was
                     originally found in the blood coagulation protein von
                     Willebrand factor (vWF). Typically, the vWA domain is made
                     up of approximately 200 amino acid residues folded into a
                     classic a/b para-rossmann type of...; cl00057"
                     /db_xref="CDD:469594"
     Site            order(11,83,113)
                     /site_type="other"
                     /note="metal ion-dependent adhesion site (MIDAS)"
                     /db_xref="CDD:238119"
     CDS             1..401
                     /gene="LOC144112642"
                     /coded_by="XM_077645452.1:542..1747"
                     /db_xref="GeneID:144112642"
ORIGIN      
        1 myavptvicv dtsdymrngd feptrldaqy eavkfvfhak trshrenkva lltlarpkvl
       61 atltnnarhf vskleemkpd gcidfvnalr taklmlrnrl drfqqmriva fvgspvtsdk
      121 kellslakkl kkenvkvdiv sfgendlnip tltpflsiln gggyagshmv svaagnvlwr
      181 vlvnspivra egdtsftyri nnftidfedd dpalalvlrv smeehrqiea rramanaang
      241 aalapssdga gampaeeell ehmsygtgqg gaftgiqhye vmteeeelav aiqmslrlcs
      301 aeiptqylpd avaaeeeple pneskeppkk aeptaaaaky dsafqdpaff sffleslpdp
      361 gsesdaacna vgdtilenqy kgadtdeden tpdkdkltkr e
//
LOCUS       XP_077501580             684 aa            linear   INV 25-NOV-2025
DEFINITION  SWI/SNF-related matrix-associated actin-dependent regulator of
            chromatin subfamily A-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077501580
VERSION     XP_077501580.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645454.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..684
                     /product="SWI/SNF-related matrix-associated
                     actin-dependent regulator of chromatin subfamily A-like
                     protein 1"
                     /calculated_mol_wt=76716
     Region          126..181
                     /region_name="HARP"
                     /note="HepA-related protein (HARP); pfam07443"
                     /db_xref="CDD:462166"
     Region          211..421
                     /region_name="DEXHc_HARP_SMARCAL1"
                     /note="DEXH-box helicase domain of SMARCAL1; cd18010"
                     /db_xref="CDD:350768"
     Site            order(235..241,271,325..326)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350768"
     Region          484..607
                     /region_name="SF2_C_SNF"
                     /note="C-terminal helicase domain of the SNF family
                     helicases; cd18793"
                     /db_xref="CDD:350180"
     Site            order(508..509,517,559,580..581)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:350180"
     Site            order(565..566,568,591,594)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350180"
     CDS             1..684
                     /gene="Marcal1"
                     /coded_by="XM_077645454.1:217..2271"
                     /db_xref="GeneID:144112645"
ORIGIN      
        1 msdtlteaqk rlieenrqka larraerlka asacgaqtdt lccptsrpal dvaqvlhkng
       61 qsgalpfngp tssgaqdlnt rsvlcqksrt tiamrnetpk avaavcnips akpvsgscvv
      121 isddrfrvda pyhqqmieif rtmpsrmyda ekkrwsfllt chnplmdklk ylrpavlvae
      181 iprhvlkaft espetcevsl asldcklera llpfqsegvr tavrrkgril iaddmglgkt
      241 iqgiavaayy reewpvlvvt pssvrfawke aflqwmpslr eeqvtvlvtg cdqidllhqv
      301 iitsydlltk kteelcnrfq vvildeshfi knpktartka cqkalskarr vvlltgtpal
      361 srpielytqi cavkpkcfps mqefgirycn gqknqwgwdf sgssnmhelq lflertvmir
      421 rlkadvlsql pakqrqvvll dpsymkstdk vmqhmaremh ntklqamekr gvllayfhet
      481 gihktkavck yiedlvegde kflcfahhqa vldsicdllt rkscshirid gktsaesrkq
      541 lcdkfqyddm crvavlsita anagitlssa slvvfaelfw npgiltqaed rvhrigqqnc
      601 vviqylvakg taddyiwpmv cskldtlgka glskdsfydv dlkvsadskq prledffpad
      661 eswvdfidad stdgcstakk tkvh
//
LOCUS       XP_077501581             486 aa            linear   INV 25-NOV-2025
DEFINITION  DNA polymerase lambda-like [Amblyomma americanum].
ACCESSION   XP_077501581
VERSION     XP_077501581.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645455.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..486
                     /product="DNA polymerase lambda-like"
                     /calculated_mol_wt=53674
     Region          2..85
                     /region_name="BRCT"
                     /note="C-terminal domain of the breast cancer suppressor
                     protein (BRCA1) and related domains; cl00038"
                     /db_xref="CDD:469589"
     Region          166..484
                     /region_name="NT_POLXc"
                     /note="Nucleotidyltransferase (NT) domain of family X DNA
                     Polymerases; cd00141"
                     /db_xref="CDD:143386"
     Site            order(252,254,256..261,264,285,299,329..330,333,335,
                     337..340,342,384,386,400,402,417..422,425,441)
                     /site_type="active"
                     /db_xref="CDD:143386"
     Site            order(252,254,256..261,264,285,329,333,339..340,342,384,
                     386,400,402,417..422,425)
                     /site_type="other"
                     /note="primer binding site [nucleotide binding]"
                     /db_xref="CDD:143386"
     Site            order(299,329..330,333,335,337..340,342,384,402,417..420,
                     422,425,441)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:143386"
     Site            order(340,342,402)
                     /site_type="metal-binding"
                     /note="metal binding triad [ion binding]"
                     /db_xref="CDD:143386"
     CDS             1..486
                     /gene="LOC144112646"
                     /coded_by="XM_077645455.1:343..1803"
                     /db_xref="GeneID:144112646"
ORIGIN      
        1 mfagtrvfvv ptsiggprvk llqsrliehg aavvcdekla thmvvaealn egrlskivdt
       61 eslqpevkiv kcawisdclk sgllletaay elrckalsta gsacsphsdv appkkarlss
      121 rrsetaspsd tsqdpepcst qrkavvsdkw acavastdvp vnknshitdq leamvetfqs
      181 tndhwralgy ekaiiqlkrh pteiatweea rslpnigerl adkiweivqq gklqkleefq
      241 sqenlvsqnl ftkiwgagps taqkwvqqgf hsleelaarg nltsqqkigl khfddflekm
      301 praeagliad mvakaalsvq pglevvpcgs yrrgramcgd vdilithpdg rshkgvlcri
      361 lqilhdsaff tddlavpheg nslkymgvcr lpgmgskhrr idifvvsqee facsllaftg
      421 sahfnrsmrl larrkgmsln dhglyadvcr mgeekigygn rlptpteesi fehlgleyrp
      481 peerdh
//
LOCUS       XP_077501582             200 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112647 [Amblyomma americanum].
ACCESSION   XP_077501582
VERSION     XP_077501582.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645456.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..200
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..200
                     /product="uncharacterized protein LOC144112647"
                     /calculated_mol_wt=22215
     CDS             1..200
                     /gene="LOC144112647"
                     /coded_by="XM_077645456.1:410..1012"
                     /db_xref="GeneID:144112647"
ORIGIN      
        1 mvlaffiasv sektsriiys tfygwndarq lgdvaltthg adssdleslr gpsemneelk
       61 trsdllkrvh qdvvedmkna ppevsssssd vtvhlterkg qfffrdadvg drivmwhsfg
      121 ecsfvaicde tentalaahc lklviqsvss hiifltpael nakigllrsi velympsgrl
      181 lfisqstvkq yeqmlhklas
//
LOCUS       XP_077501583             989 aa            linear   INV 25-NOV-2025
DEFINITION  exocyst complex component secretory 8 [Amblyomma americanum].
ACCESSION   XP_077501583
VERSION     XP_077501583.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645457.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..989
                     /product="exocyst complex component secretory 8"
                     /calculated_mol_wt=111638
     Region          <62..146
                     /region_name="Sec8_exocyst"
                     /note="Sec8 exocyst complex component specific domain;
                     pfam04048"
                     /db_xref="CDD:367782"
     CDS             1..989
                     /gene="Sec8"
                     /coded_by="XM_077645457.1:326..3295"
                     /db_xref="GeneID:144112648"
ORIGIN      
        1 manyvsssgg pvrpprtfkt tkdsngllis virslsasqn sadrdrekak lekdfkrsdq
       61 klddlvaqnk edltramqdf skissrisas rskvqcikek ltscktllhc krdelkklwl
      121 eavehkhvlq lleeieylve vpekidafls kkqylhatql lvssvnlveg slegvealkd
      181 vkgklifkke rmyedlvdel hrhlylrmtt eavqkfrrqs sdrrvqfgca tparsvppec
      241 paitpvlntg fqemrssspa stsftvgnsl sisssagdte mssllklees gkfgeaphla
      301 skytailvkc lallnriadt velikercqt ellsmvrrtt qmlldtsnvs vqtgsplqpr
      361 llmdlldllf eqfrsvamgh ecvlsnmhai veiqglengv pmytlsdvwa riqavmeyvv
      421 gkyldihntg svqqvpppvf temtatneia syftrrrnvk qkkfalfrfd csshaismnt
      481 ylkekkeemk dkcdyvevgd shqqlvcvpt sknityifkp lmsfiadiet alkledgnhc
      541 hlyeylvecv kiflghvnnd mdntidnatk sletwkvtsd peilkshgvs rpllqstlcv
      601 dqslqglqel mqalpmyveh flglactilq nyketsqvay rgivqpesed kriisatwak
      661 dedisrflms lpnwanlqlm kenrgavdve espeevrlrn kkeseilign lasgtlipph
      721 eilsdvvqlr vlaqlqesle wlssrilsma scvllaqkqr gsaylpaspr esvqdlppmp
      781 datvqtleel akefqeiadt cllvlhlevr vhcffyllpv tvqgnfaggv dsqepdpevm
      841 klnkdlssid eamtaclqqr khryifeglg hliasilins tssiqkvnen gikkvcrdif
      901 amqqnltsit mnrevaldya rqyfelfyhs pedilnlive hgaqfqemey knvllllhrs
      961 lpssdrdpds ldallsrlrd ilnevavai
//
LOCUS       XP_077501584             410 aa            linear   INV 25-NOV-2025
DEFINITION  regulatory particle non-ATPase 10 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501584
VERSION     XP_077501584.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645458.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..410
                     /product="regulatory particle non-ATPase 10 isoform X1"
                     /calculated_mol_wt=44093
     Region          1..188
                     /region_name="VWA_26S_proteasome_subunit"
                     /note="26S proteasome plays a major role in eukaryotic
                     protein breakdown, especially for ubiquitin-tagged
                     proteins. It is an ATP-dependent protease responsible for
                     the bulk of non-lysosomal proteolysis in eukaryotes, often
                     using covalent modification of...; cd01452"
                     /db_xref="CDD:238729"
     Site            order(11,13,62)
                     /site_type="active"
                     /note="partial signature motif [active]"
                     /db_xref="CDD:238729"
     Region          <238..>349
                     /region_name="DUF4045"
                     /note="Domain of unknown function (DUF4045); pfam13254"
                     /db_xref="CDD:433066"
     Region          345..392
                     /region_name="PSMD4_RAZUL"
                     /note="RAZUL (Rpn10 AZUL-binding) domain of 26S proteasome
                     non-ATPase regulatory subunit 4 (PSMD4) and similar
                     proteins; cd22297"
                     /db_xref="CDD:412092"
     Site            order(348..349,351,353..354,356,359..360,363..364,
                     366..370,374,376..377,380..381,384,387)
                     /site_type="other"
                     /note="AZUL binding site [polypeptide binding]"
                     /db_xref="CDD:412092"
     CDS             1..410
                     /gene="Rpn10"
                     /coded_by="XM_077645458.1:137..1369"
                     /db_xref="GeneID:144112649"
ORIGIN      
        1 mvlestvvcv dnsefmrngd fiptrlqaqq eavslvchsk trsnpennvg lltlassevl
       61 atlttdvgrl lsklhqvqpk gdinfltair iahlvlkhrq gknhkmrivv fvgspietds
      121 relsklakrl kkekvnvdiv nfgedninte klasfinmln gkdgggshlv tvppghqthl
      181 sdalisspvi qgedgtggig lgvggfefgv dpnedpelal alrvsmeeqr qrqedearra
      241 aansakqatq aastteaesg sseerllera lamsmetepa paattsslpd faamtedeqi
      301 ayamqmslqq aqsdssasgt apraspavne epmetdepee pkkgeaesed yemirdpafl
      361 lyvlenlpgv dpqsetvrna lgeltqgdag kksaekdkgt qdkdkssgdk
//
LOCUS       XP_077501585             343 aa            linear   INV 25-NOV-2025
DEFINITION  regulatory particle non-ATPase 10 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501585
VERSION     XP_077501585.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645459.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..343
                     /product="regulatory particle non-ATPase 10 isoform X2"
                     /calculated_mol_wt=36838
     Region          1..188
                     /region_name="VWA_26S_proteasome_subunit"
                     /note="26S proteasome plays a major role in eukaryotic
                     protein breakdown, especially for ubiquitin-tagged
                     proteins. It is an ATP-dependent protease responsible for
                     the bulk of non-lysosomal proteolysis in eukaryotes, often
                     using covalent modification of...; cd01452"
                     /db_xref="CDD:238729"
     Site            order(11,13,62)
                     /site_type="active"
                     /note="partial signature motif [active]"
                     /db_xref="CDD:238729"
     Region          <238..>343
                     /region_name="DUF4045"
                     /note="Domain of unknown function (DUF4045); pfam13254"
                     /db_xref="CDD:433066"
     CDS             1..343
                     /gene="Rpn10"
                     /coded_by="XM_077645459.1:136..1167"
                     /db_xref="GeneID:144112649"
ORIGIN      
        1 mvlestvvcv dnsefmrngd fiptrlqaqq eavslvchsk trsnpennvg lltlassevl
       61 atlttdvgrl lsklhqvqpk gdinfltair iahlvlkhrq gknhkmrivv fvgspietds
      121 relsklakrl kkekvnvdiv nfgedninte klasfinmln gkdgggshlv tvppghqthl
      181 sdalisspvi qgedgtggig lgvggfefgv dpnedpelal alrvsmeeqr qrqedearra
      241 aansakqatq aastteaesg sseerllera lamsmetepa paattsslpd faamtedeqi
      301 ayamqmslqq aqsdssasgt apraspavne epmetdepee pkk
//
LOCUS       XP_077501586             304 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112650 [Amblyomma americanum].
ACCESSION   XP_077501586
VERSION     XP_077501586.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645460.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..304
                     /product="uncharacterized protein LOC144112650"
                     /calculated_mol_wt=32014
     Region          <8..134
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..304
                     /gene="LOC144112650"
                     /coded_by="XM_077645460.1:37..951"
                     /db_xref="GeneID:144112650"
ORIGIN      
        1 mekkqeqpkr rrspptsgrs pkhpsphrch paatppgpgr agprhlgplf gqsprrrqas
       61 patlghspar splcqrllst ppargpeppa aqaprqpprd vatwtcqagt dapvgyvswr
      121 pwkrqerakr ltpparlfnv frgrtvadad qraegvslpl spgtllcpcg avmlheeahk
      181 agdlhdqrtr gattrddrhp aaatpsaaar qmlaragrea eflaarhagg ppepslgsqp
      241 saavappssa aappapsdea rdpdpkrpap alaaaelpap dmeagatpag glemdldlws
      301 teie
//
LOCUS       XP_077501587             216 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112652 [Amblyomma americanum].
ACCESSION   XP_077501587
VERSION     XP_077501587.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645461.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..216
                     /product="uncharacterized protein LOC144112652"
                     /calculated_mol_wt=22852
     CDS             1..216
                     /gene="LOC144112652"
                     /coded_by="XM_077645461.1:16028..16678"
                     /db_xref="GeneID:144112652"
ORIGIN      
        1 mmstldtdsl sifvyraadl hdtvshrgil adagtssaap qqrrgcfscg rlahmykncp
       61 haarpcmhfq nhpppflpvh vdgigeltdl vdtgaqrtal lqrhapvpls qwtssplqgk
      121 gsaipvgavd lcirteagsk vlvdvpvfad lpaglnlvad hlmsgdmals iqngevqlrs
      181 crialspsvd ehpkgeasgp qtesallhqh saifad
//
LOCUS       XP_077501588             174 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112653 [Amblyomma americanum].
ACCESSION   XP_077501588
VERSION     XP_077501588.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645462.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..174
                     /product="uncharacterized protein LOC144112653"
                     /calculated_mol_wt=19408
     CDS             1..174
                     /gene="LOC144112653"
                     /coded_by="XM_077645462.1:124..648"
                     /db_xref="GeneID:144112653"
ORIGIN      
        1 mstddcpnht lcptgeqswc rhnaakakee pepkhkyslp sdvaeallpv ysrlseksll
       61 qrciqgktqn snesfhsiiw tlihkerhss lfaveaatae avlrfnvcck htatvilqec
      121 nanvpatvsr raeekdlrrn arsakkrats lgfakaekkk hrdnmhpdya pgaf
//
LOCUS       XP_077501590             442 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112654 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501590
VERSION     XP_077501590.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645464.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..442
                     /product="uncharacterized protein LOC144112654 isoform X1"
                     /calculated_mol_wt=49339
     CDS             1..442
                     /gene="LOC144112654"
                     /coded_by="XM_077645464.1:11384..12712"
                     /db_xref="GeneID:144112654"
ORIGIN      
        1 mqqggvsnlc lplqsmaiiv emygnvghsl cslcsmlfss fliltwhfri advldepqqa
       61 eqsrvtatys ltyslpavpn diqfsierhq sgqyfkarsr vvqwlyhdlc lytmypgkly
      121 neaaqalvsr ypnladssgt gydswrealr frakyerrki riqqndgads ppskkttkdg
      181 kvatvggnal qrvsrpsvat apdgedeesi aghiegmksa vqkarpdmay iidcmrrtfp
      241 trrkwigsed psvevaiekf palamssiaq lefelitsvs vlerleealh rtkekivhaa
      301 rkkrhlegfl edfdaridgt seaavyettv taavcllpsm vkervetfvr pfdpaaahyi
      361 ptvvhrggil ttdfsvclek iciketslla avatqmalyw afnivfdkka qrsfdllcrl
      421 invdsglrpt plvrlaqtvl ge
//
LOCUS       XP_077501591             423 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112654 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501591
VERSION     XP_077501591.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645465.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..423
                     /product="uncharacterized protein LOC144112654 isoform X2"
                     /calculated_mol_wt=47010
     CDS             1..423
                     /gene="LOC144112654"
                     /coded_by="XM_077645465.1:10319..11590"
                     /db_xref="GeneID:144112654"
ORIGIN      
        1 mgrlhgagwl sawetmscsa astlppsssr iadvldepqq aeqsrvtaty sltyslpavp
       61 ndiqfsierh qsgqyfkars rvvqwlyhdl clytmypgkl yneaaqalvs rypnladssg
      121 tgydswreal rfrakyerrk iriqqndgad sppskkttkd gkvatvggna lqrvsrpsva
      181 tapdgedees iaghiegmks avqkarpdma yiidcmrrtf ptrrkwigse dpsvevaiek
      241 fpalamssia qlefelitsv svlerleeal hrtkekivha arkkrhlegf ledfdaridg
      301 tseaavyett vtaavcllps mvkervetfv rpfdpaaahy iptvvhrggi lttdfsvcle
      361 kiciketsll aavatqmaly wafnivfdkk aqrsfdllcr linvdsglrp tplvrlaqtv
      421 lge
//
LOCUS       XP_077501592             419 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112654 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501592
VERSION     XP_077501592.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645466.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..419
                     /product="uncharacterized protein LOC144112654 isoform X3"
                     /calculated_mol_wt=46842
     CDS             1..419
                     /gene="LOC144112654"
                     /coded_by="XM_077645466.1:11240..12499"
                     /db_xref="GeneID:144112654"
ORIGIN      
        1 mflltylkts kfgtgqksvs edcqvriadv ldepqqaeqs rvtatyslty slpavpndiq
       61 fsierhqsgq yfkarsrvvq wlyhdlclyt mypgklynea aqalvsrypn ladssgtgyd
      121 swrealrfra kyerrkiriq qndgadspps kkttkdgkva tvggnalqrv srpsvatapd
      181 gedeesiagh iegmksavqk arpdmayiid cmrrtfptrr kwigsedpsv evaiekfpal
      241 amssiaqlef elitsvsvle rleealhrtk ekivhaarkk rhlegfledf daridgtsea
      301 avyettvtaa vcllpsmvke rvetfvrpfd paaahyiptv vhrggilttd fsvclekici
      361 ketsllaava tqmalywafn ivfdkkaqrs fdllcrlinv dsglrptplv rlaqtvlge
//
LOCUS       XP_077501593             329 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112654 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077501593
VERSION     XP_077501593.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645467.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..329
                     /product="uncharacterized protein LOC144112654 isoform X4"
                     /calculated_mol_wt=36462
     CDS             1..329
                     /gene="LOC144112654"
                     /coded_by="XM_077645467.1:698..1687"
                     /db_xref="GeneID:144112654"
ORIGIN      
        1 mypgklynea aqalvsrypn ladssgtgyd swrealrfra kyerrkiriq qndgadspps
       61 kkttkdgkva tvggnalqrv srpsvatapd gedeesiagh iegmksavqk arpdmayiid
      121 cmrrtfptrr kwigsedpsv evaiekfpal amssiaqlef elitsvsvle rleealhrtk
      181 ekivhaarkk rhlegfledf daridgtsea avyettvtaa vcllpsmvke rvetfvrpfd
      241 paaahyiptv vhrggilttd fsvclekici ketsllaava tqmalywafn ivfdkkaqrs
      301 fdllcrlinv dsglrptplv rlaqtvlge
//
LOCUS       XP_077501594             329 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112654 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077501594
VERSION     XP_077501594.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645468.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..329
                     /product="uncharacterized protein LOC144112654 isoform X4"
                     /calculated_mol_wt=36462
     CDS             1..329
                     /gene="LOC144112654"
                     /coded_by="XM_077645468.1:701..1690"
                     /db_xref="GeneID:144112654"
ORIGIN      
        1 mypgklynea aqalvsrypn ladssgtgyd swrealrfra kyerrkiriq qndgadspps
       61 kkttkdgkva tvggnalqrv srpsvatapd gedeesiagh iegmksavqk arpdmayiid
      121 cmrrtfptrr kwigsedpsv evaiekfpal amssiaqlef elitsvsvle rleealhrtk
      181 ekivhaarkk rhlegfledf daridgtsea avyettvtaa vcllpsmvke rvetfvrpfd
      241 paaahyiptv vhrggilttd fsvclekici ketsllaava tqmalywafn ivfdkkaqrs
      301 fdllcrlinv dsglrptplv rlaqtvlge
//
LOCUS       XP_077501595             329 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112654 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077501595
VERSION     XP_077501595.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645469.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..329
                     /product="uncharacterized protein LOC144112654 isoform X4"
                     /calculated_mol_wt=36462
     CDS             1..329
                     /gene="LOC144112654"
                     /coded_by="XM_077645469.1:421..1410"
                     /db_xref="GeneID:144112654"
ORIGIN      
        1 mypgklynea aqalvsrypn ladssgtgyd swrealrfra kyerrkiriq qndgadspps
       61 kkttkdgkva tvggnalqrv srpsvatapd gedeesiagh iegmksavqk arpdmayiid
      121 cmrrtfptrr kwigsedpsv evaiekfpal amssiaqlef elitsvsvle rleealhrtk
      181 ekivhaarkk rhlegfledf daridgtsea avyettvtaa vcllpsmvke rvetfvrpfd
      241 paaahyiptv vhrggilttd fsvclekici ketsllaava tqmalywafn ivfdkkaqrs
      301 fdllcrlinv dsglrptplv rlaqtvlge
//
LOCUS       XP_077501596             302 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112656 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501596
VERSION     XP_077501596.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645470.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..302
                     /product="uncharacterized protein LOC144112656 isoform X1"
                     /calculated_mol_wt=33468
     Region          60..>103
                     /region_name="DUF3432"
                     /note="Domain of unknown function (DUF3432); pfam11914"
                     /db_xref="CDD:403204"
     Region          110..187
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..302
                     /gene="LOC144112656"
                     /coded_by="XM_077645470.1:757..1665"
                     /db_xref="GeneID:144112656"
ORIGIN      
        1 mavadvsaam lvlidgvetr vyavldehgt hmknddgfly etegglrisv lrveepmrnt
       61 ppptvpaave dssrgapspl wqttpppvcq ptpspgpscy spppsggger dawserksrf
      121 litkykeakd nigrkggfrt kkamwekltd qinsefgchl tplqvenkwk tmeraykksk
      181 akngssghsr aaclyerels evlerehhit pvallapgri vanreeenml pnngpddeed
      241 eppapsrste atparrrsrq dktaigglik vlqeamaekk qqheekmrll erlvkaveak
      301 nk
//
LOCUS       XP_077501597             240 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112656 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501597
VERSION     XP_077501597.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645471.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..240
                     /product="uncharacterized protein LOC144112656 isoform X2"
                     /calculated_mol_wt=26232
     Region          <57..>98
                     /region_name="Med15"
                     /note="ARC105 or Med15 subunit of Mediator complex
                     non-fungal; pfam09606"
                     /db_xref="CDD:312941"
     Region          110..187
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..240
                     /gene="LOC144112656"
                     /coded_by="XM_077645471.1:757..1479"
                     /db_xref="GeneID:144112656"
ORIGIN      
        1 mavadvsaam lvlidgvetr vyavldehgt hmknddgfly etegglrisv lrveepmrnt
       61 ppptvpaave dssrgapspl wqttpppvcq ptpspgpscy spppsggger dawserksrf
      121 litkykeakd nigrkggfrt kkamwekltd qinsefgchl tplqvenkwk tmeraykksk
      181 akngssghsr aaclyerels evlerehhit pvallapgri vangekpsyp tanfsatpfa
//
LOCUS       XP_077501598             501 aa            linear   INV 25-NOV-2025
DEFINITION  M-phase inducer phosphatase 1-B-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501598
VERSION     XP_077501598.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645472.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..501
                     /product="M-phase inducer phosphatase 1-B-like isoform X1"
                     /calculated_mol_wt=54747
     Region          <248..489
                     /region_name="MIH1"
                     /note="Mitotic inducer, protein phosphatase [Cell division
                     and chromosome partitioning]; COG5105"
                     /db_xref="CDD:227436"
     Region          333..450
                     /region_name="Cdc25"
                     /note="Cdc25 phosphatases are members of the Rhodanese
                     Homology Domain superfamily. They activate the cell
                     division kinases throughout the cell cycle progression.
                     Cdc25 phosphatases dephosphorylate phosphotyrosine and
                     phosphothreonine residues, in order to...; cd01530"
                     /db_xref="CDD:238788"
     Site            406..412
                     /site_type="active"
                     /note="oxyanion binding site [active]"
                     /db_xref="CDD:238788"
     Site            406
                     /site_type="active"
                     /note="active site residue [active]"
                     /db_xref="CDD:238788"
     CDS             1..501
                     /gene="LOC144112662"
                     /coded_by="XM_077645472.1:245..1750"
                     /db_xref="GeneID:144112662"
ORIGIN      
        1 msspskigsp mwdgdlelsp vgglarnlte lswspatqtp ksggltgegs sfalplpkyp
       61 lahvmkvees spslqycvlk dvsnrkdltg rpsgwrrrll vgghhdndeg fqsagslfss
      121 agsqensssn gsgsciksst aatsvswddh sgagasgcts ssedgflcsl ddsfqeheph
      181 lpgpiakllh splyqasqtl lanelkenvl npcenspacg sseesadasq sellgtplta
      241 llnpymafkr pvspgnqpfs pshkryrgyq lqrftsysep scssrvanfs rslsdtdasi
      301 klalqksdad pdligdftkp yalplvkgkh hdlkmilpst ladvlqgkyl svvdevtiid
      361 crypfeyegg hikgainvfs sedllsefle kaaptpdsrr vlvfhcefss ergpklsrll
      421 rekdrqiney palkhpeiyv leggykafye eyqewctpqg yipmrhkghe tdlrryrsma
      481 kteaassksr aalhiralkk f
//
LOCUS       XP_077501599             437 aa            linear   INV 25-NOV-2025
DEFINITION  M-phase inducer phosphatase 1-B-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501599
VERSION     XP_077501599.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645473.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..437
                     /product="M-phase inducer phosphatase 1-B-like isoform X2"
                     /calculated_mol_wt=48141
     Region          <184..425
                     /region_name="MIH1"
                     /note="Mitotic inducer, protein phosphatase [Cell division
                     and chromosome partitioning]; COG5105"
                     /db_xref="CDD:227436"
     Region          269..386
                     /region_name="Cdc25"
                     /note="Cdc25 phosphatases are members of the Rhodanese
                     Homology Domain superfamily. They activate the cell
                     division kinases throughout the cell cycle progression.
                     Cdc25 phosphatases dephosphorylate phosphotyrosine and
                     phosphothreonine residues, in order to...; cd01530"
                     /db_xref="CDD:238788"
     Site            342..348
                     /site_type="active"
                     /note="oxyanion binding site [active]"
                     /db_xref="CDD:238788"
     Site            342
                     /site_type="active"
                     /note="active site residue [active]"
                     /db_xref="CDD:238788"
     CDS             1..437
                     /gene="LOC144112662"
                     /coded_by="XM_077645473.1:224..1537"
                     /db_xref="GeneID:144112662"
ORIGIN      
        1 mkveesspsl qycvlkdvsn rkdltgrpsg wrrrllvggh hdndegfqsa gslfssagsq
       61 ensssngsgs ciksstaats vswddhsgag asgctsssed gflcslddsf qehephlpgp
      121 iakllhsply qasqtllane lkenvlnpce nspacgssee sadasqsell gtpltallnp
      181 ymafkrpvsp gnqpfspshk ryrgyqlqrf tsysepscss rvanfsrsls dtdasiklal
      241 qksdadpdli gdftkpyalp lvkgkhhdlk milpstladv lqgkylsvvd evtiidcryp
      301 feyegghikg ainvfssedl lseflekaap tpdsrrvlvf hcefssergp klsrllrekd
      361 rqineypalk hpeiyvlegg ykafyeeyqe wctpqgyipm rhkghetdlr ryrsmaktea
      421 assksraalh iralkkf
//
LOCUS       XP_077501600             403 aa            linear   INV 25-NOV-2025
DEFINITION  M-phase inducer phosphatase 1-B-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501600
VERSION     XP_077501600.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645474.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..403
                     /product="M-phase inducer phosphatase 1-B-like isoform X3"
                     /calculated_mol_wt=44269
     Region          <150..391
                     /region_name="MIH1"
                     /note="Mitotic inducer, protein phosphatase [Cell division
                     and chromosome partitioning]; COG5105"
                     /db_xref="CDD:227436"
     Region          235..352
                     /region_name="Cdc25"
                     /note="Cdc25 phosphatases are members of the Rhodanese
                     Homology Domain superfamily. They activate the cell
                     division kinases throughout the cell cycle progression.
                     Cdc25 phosphatases dephosphorylate phosphotyrosine and
                     phosphothreonine residues, in order to...; cd01530"
                     /db_xref="CDD:238788"
     Site            308..314
                     /site_type="active"
                     /note="oxyanion binding site [active]"
                     /db_xref="CDD:238788"
     Site            308
                     /site_type="active"
                     /note="active site residue [active]"
                     /db_xref="CDD:238788"
     CDS             1..403
                     /gene="LOC144112662"
                     /coded_by="XM_077645474.1:114..1325"
                     /db_xref="GeneID:144112662"
ORIGIN      
        1 mralghhdnd egfqsagslf ssagsqenss sngsgsciks staatsvswd dhsgagasgc
       61 tsssedgflc slddsfqehe phlpgpiakl lhsplyqasq tllanelken vlnpcenspa
      121 cgsseesada sqsellgtpl tallnpymaf krpvspgnqp fspshkryrg yqlqrftsys
      181 epscssrvan fsrslsdtda siklalqksd adpdligdft kpyalplvkg khhdlkmilp
      241 stladvlqgk ylsvvdevti idcrypfeye gghikgainv fssedllsef lekaaptpds
      301 rrvlvfhcef ssergpklsr llrekdrqin eypalkhpei yvleggykaf yeeyqewctp
      361 qgyipmrhkg hetdlrryrs makteaassk sraalhiral kkf
//
LOCUS       XP_077501601             237 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: clarin-2 [Amblyomma americanum].
ACCESSION   XP_077501601
VERSION     XP_077501601.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645475.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..237
                     /product="LOW QUALITY PROTEIN: clarin-2"
                     /calculated_mol_wt=26262
     CDS             1..237
                     /gene="LOC144112663"
                     /coded_by="XM_077645475.1:139..852"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 1 base in 1 codon"
                     /db_xref="GeneID:144112663"
ORIGIN      
        1 mdlvqrvftf ltffvccgcl afltvslatq ewieakpvml vyvsndslql aenegkfrge
       61 vsfglfhgkk tlnyglgprh stfsvkdelq knpalmifgl wlvtvlgxsl avlfglvsci
      121 faivnsvmtp vetitgrtgl ylwntvgglf clvaiiawvv qfylklshnv mtqdeqkdkw
      181 tsegraslgy sfyflvvalv lfslnvcilv vaslqpwgwk kpkhispknp egvimly
//
LOCUS       XP_077501602             198 aa            linear   INV 25-NOV-2025
DEFINITION  stem-loop binding protein [Amblyomma americanum].
ACCESSION   XP_077501602
VERSION     XP_077501602.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645476.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..198
                     /product="stem-loop binding protein"
                     /calculated_mol_wt=22765
     Region          63..131
                     /region_name="SLBP_RNA_bind"
                     /note="Histone RNA hairpin-binding protein RNA-binding
                     domain; pfam15247"
                     /db_xref="CDD:464587"
     CDS             1..198
                     /gene="Slbp"
                     /coded_by="XM_077645476.1:230..826"
                     /db_xref="GeneID:144112664"
ORIGIN      
        1 mdasvgarap egqrmaagdf wdlqerless wsdimekrdk vavnkrkntf aehkssslql
       61 yetdpevlsr rmkqidygks tvgykrycas vprknrakhh prtpnrylky srrswdaqir
      121 iwrrqlhiwd ppkeveasde llddsldldd advyldedvk alledvkppl gngteailse
      181 adssakplee vtkkldfe
//
LOCUS       XP_077501603             666 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112665 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501603
VERSION     XP_077501603.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645477.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..666
                     /product="uncharacterized protein LOC144112665 isoform X1"
                     /calculated_mol_wt=70901
     Region          592..658
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(614,617,632,634,637,640,651,654)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     CDS             1..666
                     /gene="LOC144112665"
                     /coded_by="XM_077645477.1:74..2074"
                     /db_xref="GeneID:144112665"
ORIGIN      
        1 msvaspqhke saastvsryl lpkpprhspp llpspgppvs hhgfspdpqa clqeirqlas
       61 ppagsgdghr spasepllle gapasmsasm vhrtspggyd lrahrraaap pelsagplsp
      121 lyfsnlrppe dsrqkasesp srkrlklslg aaaaeiasap aavpiappga wgegsptlrr
      181 rsashqqrrm dhpphhsarc tsntarcrrs psmrrpryre ppppvaqhaw rpnpptpeqq
      241 rgfqphlhhp mahhhhhvgh hhhhhgggaa ggaqassraa gttgvrvawe psvgtlfsaa
      301 spvqafpqap sfvfdlaqvp vtlasavpaq pfavcsttss iasgggplga agaaggaaga
      361 phyhlplftp sffaapllaa sghhhhvapc gccfhhhqfa apaflhpthq mmaaaaapla
      421 thqrapqhat llpqsqepdl ellaeqrrng gaygglspqi ghhaapgths psppishllp
      481 davttvtpqs dficmvhprr lvhgrrssgr rwraplmaat pppppppphp gfllhlfwwp
      541 ydgssamlsn npmpayapem ataespaaen yeallnlaer lgeakprglp raeieqllsy
      601 rfnpethese qtscvvcmcd fearqvlrvl pcahefharc vdkwlknnrt cpicrgdase
      661 gghsse
//
LOCUS       XP_077501604             594 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112665 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501604
VERSION     XP_077501604.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645478.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..594
                     /product="uncharacterized protein LOC144112665 isoform X2"
                     /calculated_mol_wt=63818
     Region          520..586
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(542,545,560,562,565,568,579,582)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     CDS             1..594
                     /gene="LOC144112665"
                     /coded_by="XM_077645478.1:75..1859"
                     /db_xref="GeneID:144112665"
ORIGIN      
        1 mdragssrgp patrrtkrrs eeprlseepr ldveqpgtre lsagplsply fsnlrppeds
       61 rqkasespsr krlklslgaa aaeiasapaa vpiappgawg egsptlrrrs ashqqrrmdh
      121 pphhsarcts ntarcrrsps mrrpryrepp ppvaqhawrp npptpeqqrg fqphlhhpma
      181 hhhhhvghhh hhhgggaagg aqassraagt tgvrvaweps vgtlfsaasp vqafpqapsf
      241 vfdlaqvpvt lasavpaqpf avcsttssia sgggplgaag aaggaagaph yhlplftpsf
      301 faapllaasg hhhhvapcgc cfhhhqfaap aflhpthqmm aaaaaplath qrapqhatll
      361 pqsqepdlel laeqrrngga ygglspqigh haapgthsps ppishllpda vttvtpqsdf
      421 icmvhprrlv hgrrssgrrw raplmaatpp pppppphpgf llhlfwwpyd gssamlsnnp
      481 mpayapemat aespaaenye allnlaerlg eakprglpra eieqllsyrf npetheseqt
      541 scvvcmcdfe arqvlrvlpc ahefharcvd kwlknnrtcp icrgdasegg hsse
//
LOCUS       XP_077501605             591 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112665 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501605
VERSION     XP_077501605.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645479.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..591
                     /product="uncharacterized protein LOC144112665 isoform X3"
                     /calculated_mol_wt=63504
     Region          517..583
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(539,542,557,559,562,565,576,579)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     CDS             1..591
                     /gene="LOC144112665"
                     /coded_by="XM_077645479.1:130..1905"
                     /db_xref="GeneID:144112665"
ORIGIN      
        1 mdragssrgp patrrtkrrs eeprlseepr ldveqpelsa gplsplyfsn lrppedsrqk
       61 asespsrkrl klslgaaaae iasapaavpi appgawgegs ptlrrrsash qqrrmdhpph
      121 hsarctsnta rcrrspsmrr pryreppppv aqhawrpnpp tpeqqrgfqp hlhhpmahhh
      181 hhvghhhhhh gggaaggaqa ssraagttgv rvawepsvgt lfsaaspvqa fpqapsfvfd
      241 laqvpvtlas avpaqpfavc sttssiasgg gplgaagaag gaagaphyhl plftpsffaa
      301 pllaasghhh hvapcgccfh hhqfaapafl hpthqmmaaa aaplathqra pqhatllpqs
      361 qepdlellae qrrnggaygg lspqighhaa pgthspsppi shllpdavtt vtpqsdficm
      421 vhprrlvhgr rssgrrwrap lmaatppppp ppphpgfllh lfwwpydgss amlsnnpmpa
      481 yapemataes paaenyeall nlaerlgeak prglpraeie qllsyrfnpe theseqtscv
      541 vcmcdfearq vlrvlpcahe fharcvdkwl knnrtcpicr gdasegghss e
//
LOCUS       XP_077501606             309 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112668 [Amblyomma americanum].
ACCESSION   XP_077501606
VERSION     XP_077501606.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645480.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..309
                     /product="uncharacterized protein LOC144112668"
                     /calculated_mol_wt=35111
     Region          110..300
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(166,205,219,221)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..309
                     /gene="LOC144112668"
                     /coded_by="XM_077645480.1:790..1719"
                     /db_xref="GeneID:144112668"
ORIGIN      
        1 mvpvdqlnls kpgrkarkla aeanytsety dardieifqr lrkasssavv lrsipktdse
       61 dtdsasedee mytflrnark arstkedfle dmflplseie riekatrlts ksplwheary
      121 grltgttmhr irtlkadtnv qglldsvlqr rpplnieavr wgadkekvak eqylefmkah
      181 hanfelheig fvidtdapfh gatpdglwhc gchgvgllet kcprslkeph hqvleasyls
      241 asgtlletht yftqvqtqlh vcrkavchfv vwlphqfgiv evpysshffk pllekaravw
      301 aehvlpall
//
LOCUS       XP_077501607            1252 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112669 [Amblyomma americanum].
ACCESSION   XP_077501607
VERSION     XP_077501607.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645481.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1252
                     /product="uncharacterized protein LOC144112669"
                     /calculated_mol_wt=138841
     Region          <42..244
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          645..1248
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..1252
                     /gene="LOC144112669"
                     /coded_by="XM_077645481.1:993..4751"
                     /db_xref="GeneID:144112669"
ORIGIN      
        1 mrwrltlpkk kkdtvragrv maskkdsasp sdtceafvke hpsdspaata spagevdkkp
       61 gsrtgpmdaa spnprdtpvs atvrvrlgpt gangpveave daeehtpsds esteespqqe
      121 sptdgqgslr aerkpiqlkp qfdlpkiats prqsstelqp dsyqhgvqgr pskdhssiss
      181 flkknahpta lgsprpgpst llprthgrkv mndgvrgmra peivkqlesy ptatppsgqr
      241 tpdlkvlrit iprfagesky lagkyfqssg tqmsnnarpe sgtppattfe dsaaserrig
      301 ggprrkgras pgassasssl tslviggnrr psfserdqqm qefnnrrdps erpgtstasd
      361 aicrdvkssr sfptsfypam sghcsgkdta repkankmif rptgslsgsk hssqksclre
      421 ylrdkralce gdkpqgrasl sgrslsstsf slrgtpehsr asmpsygrhs prristtltg
      481 dsperrcgrr rhqshkfepi rvrippifpr srssssegqs aqslvsskst rsdsplhqas
      541 rrqsihpayl eatrsvartp qfvssttqgy sgnrgvkalf vsqppvvaif gvvvlvavlv
      601 aslfllvlft rpkvsqwhdt mrvcrtkrcl eyarrlrntl ntslnpcesf thfvcdgwrr
      661 rnrlsvreea flgvvrklss lllsvpvpst gqaplqraaa fyrscddvlq gradelrkvk
      721 ralldagimw prlpkdsnrs dllstwfhas irlhwspllh vmlqqdpnvt tvaleplsdf
      781 erillkhkgl egsdheakvy feelrrefla nrehdngkkd nttvtftitk qlvethlnpl
      841 gdafestqlf drgldtafmf rmvpgltkqr wlqeldyylg nserdvafvs ekprfvrafm
      901 glwrlhgedq mhlffswciv qtaalfanqk lllnfhtskm raelyhgalc lskvyllsgr
      961 evfgayydev lsgsakrsaq llvvdtrkvf adrlehwrlw dplrivvqew nssdvvlgyf
     1021 dprfdqrslk pkqdeptfpd mntslvdnwq avnldhvdan dlavsvrief adwftllpgr
     1081 dlallpyafs fpsldesaap amnhgglgyr vasalsrlft dsyiispeag fsigrslqca
     1141 anrsgergnl ypvlrqleha lalevslqay rqrsaasegy dellegfegy ssaalffiaa
     1201 cystclgser afpeagddec deifrnvqgf sdaigcapgs pmnpfvkcgi lt
//
LOCUS       XP_077501608             235 aa            linear   INV 25-NOV-2025
DEFINITION  U-scoloptoxin(11)-Sm5a-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501608
VERSION     XP_077501608.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645482.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..235
                     /product="U-scoloptoxin(11)-Sm5a-like isoform X1"
                     /calculated_mol_wt=25794
     CDS             1..235
                     /gene="LOC144112671"
                     /coded_by="XM_077645482.1:137..844"
                     /db_xref="GeneID:144112671"
ORIGIN      
        1 mdrgglpcwv llcavclaqa rprfyglapm kprsltvnvs glpecmedsa cayvqlnlag
       61 inseqlcrcp dglscpldwd pldgrtvshg ndqykycgra prlarclrdq vvystavkls
      121 lltgtklent arlhcscppt hifyrnqtsh qqydngvtai dvstlckrpp tcepveedac
      181 maisesfvtg tvfvkrdcac pagmrcptdl dlaadrvela kgtyylmncl peerd
//
LOCUS       XP_077501609             231 aa            linear   INV 25-NOV-2025
DEFINITION  U-scoloptoxin(11)-Sm5a-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077501609
VERSION     XP_077501609.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645483.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..231
                     /product="U-scoloptoxin(11)-Sm5a-like isoform X2"
                     /calculated_mol_wt=25394
     CDS             1..231
                     /gene="LOC144112671"
                     /coded_by="XM_077645483.1:137..832"
                     /db_xref="GeneID:144112671"
ORIGIN      
        1 mdrgglpcwv llcavclaqa rprfyglapm kprsltglpe cmedsacayv qlnlaginse
       61 qlcrcpdgls cpldwdpldg rtvshgndqy kycgraprla rclrdqvvys tavklslltg
      121 tklentarlh cscppthify rnqtshqqyd ngvtaidvst lckrpptcep veedacmais
      181 esfvtgtvfv krdcacpagm rcptdldlaa drvelakgty ylmnclpeer d
//
LOCUS       XP_077501610             262 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112672 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501610
VERSION     XP_077501610.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645484.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..262
                     /product="uncharacterized protein LOC144112672 isoform X1"
                     /calculated_mol_wt=27597
     Region          53..165
                     /region_name="TNFRSF"
                     /note="Tumor necrosis factor receptor superfamily
                     (TNFRSF); cl22855"
                     /db_xref="CDD:473981"
     Region          91..130
                     /region_name="CRD2"
                     /note="CRD2 [structural motif]"
                     /db_xref="CDD:276900"
     CDS             1..262
                     /gene="LOC144112672"
                     /coded_by="XM_077645484.1:496..1284"
                     /db_xref="GeneID:144112672"
ORIGIN      
        1 mvfvsqdmtk elmhfntpts knrmlsgtak lfvlfafvsq vsdtsanncs sqceacvpgt
       61 yassacdccs ipghcpkppa hsafdtlcvp csagtfcrqa gcpsclpcpp gsstteqrgq
      121 nctpceagfy kpflgpgacg pcsqgsytll kgstecivcp agfyclcthc gplpcpddav
      181 cpvgsihpts chkplfvkhg yscyisdsai giiigvcisv iivagflsar cyyfrqkhqs
      241 qnpteisslv aesrrdpvyt gl
//
LOCUS       XP_077501611             262 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112672 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501611
VERSION     XP_077501611.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645485.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..262
                     /product="uncharacterized protein LOC144112672 isoform X2"
                     /calculated_mol_wt=27598
     Region          53..165
                     /region_name="TNFRSF"
                     /note="Tumor necrosis factor receptor superfamily
                     (TNFRSF); cl22855"
                     /db_xref="CDD:473981"
     Region          91..130
                     /region_name="CRD2"
                     /note="CRD2 [structural motif]"
                     /db_xref="CDD:276900"
     CDS             1..262
                     /gene="LOC144112672"
                     /coded_by="XM_077645485.1:591..1379"
                     /db_xref="GeneID:144112672"
ORIGIN      
        1 mvfvsqdmtk elmhfntpts knrmlsgtak lfvlfafvsq vsdtsanncs sqceacvpgt
       61 yassacdccs ipghcpkppa hsafdtlcvp csagtfcrea gcpsclpcpp gsstteqrgq
      121 nctpceagfy kpflgpgacg pcsqgsytll kgstecivcp agfyclcthc gplpcpddav
      181 cpvgsihpts chkplfvkhg yscyisdsai giiigvcisv iivagflsar cyyfrqkhqs
      241 qnpteisslv aesrrdpvyt gl
//
LOCUS       XP_077501612             233 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112672 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501612
VERSION     XP_077501612.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645486.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..233
                     /product="uncharacterized protein LOC144112672 isoform X3"
                     /calculated_mol_wt=24701
     Region          59..>143
                     /region_name="TNFRSF"
                     /note="Tumor necrosis factor receptor superfamily
                     (TNFRSF); cl22855"
                     /db_xref="CDD:473981"
     Region          79..127
                     /region_name="CRD2"
                     /note="CRD2 [structural motif]"
                     /db_xref="CDD:276900"
     CDS             1..233
                     /gene="LOC144112672"
                     /coded_by="XM_077645486.1:500..1201"
                     /db_xref="GeneID:144112672"
ORIGIN      
        1 mvfvsqdmtk elmhfntpts knrmlsgtak lfvlfacdcc sipghcpkpp ahsafdtlcv
       61 pcsagtfcrq agcpsclpcp pgsstteqrg qnctpceagf ykpflgpgac gpcsqgsytl
      121 lkgstecivc pagfyclcth cgplpcpdda vcpvgsihpt schkplfvkh gyscyisdsa
      181 igiiigvcis viivagflsa rcyyfrqkhq sqnpteissl vaesrrdpvy tgl
//
LOCUS       XP_077501613             657 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial sodium/calcium exchanger protein-like [Amblyomma
            americanum].
ACCESSION   XP_077501613
VERSION     XP_077501613.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645487.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..657
                     /product="mitochondrial sodium/calcium exchanger
                     protein-like"
                     /calculated_mol_wt=70752
     Region          28..644
                     /region_name="PLN03151"
                     /note="cation/calcium exchanger; Provisional"
                     /db_xref="CDD:215604"
     CDS             1..657
                     /gene="LOC144112673"
                     /coded_by="XM_077645487.1:355..2328"
                     /db_xref="GeneID:144112673"
ORIGIN      
        1 mgtsalayaa sesdcslvkt landstgrca lvrilddcda graqlryvdf lycgdksatd
       61 aaqvllvlwl atlfvalgst assylcpalv hiskslqlsq gvagvtflaf gngapdtiat
      121 iasirrdrta laigelfggg tyvativvgl ifissnfeli qssllrdiif ylaasfwvft
      181 lylkgciqla dsvgfivlyl vyitvavwgq kflenvsklc hrravsrrvs grsqstctad
      241 knistspssp csssssssss srrssirsss kgeespdlas laeaddvvkt pppflsrprl
      301 rrsssgislh hhhenaiyyf tasveelrae paspeerrps gasqasfnfv rtsssvfpge
      361 atpllsqslg ekvppaygew tefllhispv epeewkgkrl wakiyevltl pislvlvlti
      421 pvvdhenrra nwsrplnalh cvtgpivalg vfgvltvrag dlvpvwclvi vmglglallv
      481 wftssaqeap ryhlvfayag famsiiwiyg iateivallk afgslygvsd vllgmtvlaw
      541 gnnigdlvtn lslarqgfpq mamaacfagp vlalllgigi afsmnlasra vscvelhyse
      601 llpliygalv vvlfvlllsa lvtwfrssrv vgvflillya sfllttglve fklirkg
//
LOCUS       XP_077501614             692 aa            linear   INV 25-NOV-2025
DEFINITION  myosin light chain kinase, smooth muscle-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501614
VERSION     XP_077501614.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645488.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..692
                     /product="myosin light chain kinase, smooth muscle-like
                     isoform X1"
                     /calculated_mol_wt=74467
     Region          127..217
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          143..147
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          156..160
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          183..187
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          197..202
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          210..213
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          359..609
                     /region_name="STKc_MLCK"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Myosin Light Chain Kinase; cd14103"
                     /db_xref="CDD:271005"
     Site            order(359..363,365,367,380,382,412,428..429,431,435,437,
                     475,477,479..480,482,494..495,498,513..516)
                     /site_type="active"
                     /db_xref="CDD:271005"
     Site            order(359..363,365,367,380,382,412,428..429,431,435,
                     479..480,482,494..495)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271005"
     Site            order(363,435,437,475,477,479,498,513..516)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271005"
     Site            order(494..502,505..516)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271005"
     CDS             1..692
                     /gene="LOC144112674"
                     /coded_by="XM_077645488.1:1544..3622"
                     /db_xref="GeneID:144112674"
ORIGIN      
        1 maaaaasrcg adhkesfnpc qsasgqtfhp scgaspynae ttdsaavpsr qasqdtvsqe
       61 shapcavacp lqpesshnks rdpheivtgn iqqcaaalgt adsptglqtd edstrrpcng
      121 drpqaakivr gpesvvavig shvrlgvhfv gtpaprvtwt kggkaavlpg drvdvsvdqp
      181 gscelrirqa ccddsgkyqv vvenslgsdf afasvkvegp pdppaeapra vslpsgviev
      241 twscspfdgg criqrylvda spasaqpraw srvaavlstc crlanlppaa sayvfvvraq
      301 narglsmasa esppaaplsp lgnddeeald ddviadpgpp vtlrpgeefc qdyhvheavg
      361 rgrfgvvhrc trladgtdaa akvvrcirtt erdrmrhela ilnllrghpr llrlldafek
      421 preiilvtey vsggelferv iaddfvltew dcvlflrqic agmaymhsrh vihldlkpen
      481 ilcqsqvghk iklidfglaq lyepssslrv mfgtpefvap evvsyepvsp atdmwsvgvi
      541 cyvllsglsp fmgdsdtdtf nnivrvdldf ddpvfetisa vakdfmcqli vknprkrmsa
      601 eqclehewlr vvakrgalpl ptdklkkfvi rrkwlksgna lkalsrmtnl sgqrpahgde
      661 aldllaceed apvsqpppsd asataaagee ad
//
LOCUS       XP_077501615             692 aa            linear   INV 25-NOV-2025
DEFINITION  myosin light chain kinase, smooth muscle-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501615
VERSION     XP_077501615.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645489.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..692
                     /product="myosin light chain kinase, smooth muscle-like
                     isoform X1"
                     /calculated_mol_wt=74467
     Region          127..217
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          143..147
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          156..160
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          183..187
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          197..202
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          210..213
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          359..609
                     /region_name="STKc_MLCK"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Myosin Light Chain Kinase; cd14103"
                     /db_xref="CDD:271005"
     Site            order(359..363,365,367,380,382,412,428..429,431,435,437,
                     475,477,479..480,482,494..495,498,513..516)
                     /site_type="active"
                     /db_xref="CDD:271005"
     Site            order(359..363,365,367,380,382,412,428..429,431,435,
                     479..480,482,494..495)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271005"
     Site            order(363,435,437,475,477,479,498,513..516)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271005"
     Site            order(494..502,505..516)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271005"
     CDS             1..692
                     /gene="LOC144112674"
                     /coded_by="XM_077645489.1:905..2983"
                     /db_xref="GeneID:144112674"
ORIGIN      
        1 maaaaasrcg adhkesfnpc qsasgqtfhp scgaspynae ttdsaavpsr qasqdtvsqe
       61 shapcavacp lqpesshnks rdpheivtgn iqqcaaalgt adsptglqtd edstrrpcng
      121 drpqaakivr gpesvvavig shvrlgvhfv gtpaprvtwt kggkaavlpg drvdvsvdqp
      181 gscelrirqa ccddsgkyqv vvenslgsdf afasvkvegp pdppaeapra vslpsgviev
      241 twscspfdgg criqrylvda spasaqpraw srvaavlstc crlanlppaa sayvfvvraq
      301 narglsmasa esppaaplsp lgnddeeald ddviadpgpp vtlrpgeefc qdyhvheavg
      361 rgrfgvvhrc trladgtdaa akvvrcirtt erdrmrhela ilnllrghpr llrlldafek
      421 preiilvtey vsggelferv iaddfvltew dcvlflrqic agmaymhsrh vihldlkpen
      481 ilcqsqvghk iklidfglaq lyepssslrv mfgtpefvap evvsyepvsp atdmwsvgvi
      541 cyvllsglsp fmgdsdtdtf nnivrvdldf ddpvfetisa vakdfmcqli vknprkrmsa
      601 eqclehewlr vvakrgalpl ptdklkkfvi rrkwlksgna lkalsrmtnl sgqrpahgde
      661 aldllaceed apvsqpppsd asataaagee ad
//
LOCUS       XP_077501616             692 aa            linear   INV 25-NOV-2025
DEFINITION  myosin light chain kinase, smooth muscle-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501616
VERSION     XP_077501616.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645490.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..692
                     /product="myosin light chain kinase, smooth muscle-like
                     isoform X1"
                     /calculated_mol_wt=74467
     Region          127..217
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          143..147
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          156..160
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          183..187
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          197..202
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          210..213
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          359..609
                     /region_name="STKc_MLCK"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Myosin Light Chain Kinase; cd14103"
                     /db_xref="CDD:271005"
     Site            order(359..363,365,367,380,382,412,428..429,431,435,437,
                     475,477,479..480,482,494..495,498,513..516)
                     /site_type="active"
                     /db_xref="CDD:271005"
     Site            order(359..363,365,367,380,382,412,428..429,431,435,
                     479..480,482,494..495)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271005"
     Site            order(363,435,437,475,477,479,498,513..516)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271005"
     Site            order(494..502,505..516)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271005"
     CDS             1..692
                     /gene="LOC144112674"
                     /coded_by="XM_077645490.1:679..2757"
                     /db_xref="GeneID:144112674"
ORIGIN      
        1 maaaaasrcg adhkesfnpc qsasgqtfhp scgaspynae ttdsaavpsr qasqdtvsqe
       61 shapcavacp lqpesshnks rdpheivtgn iqqcaaalgt adsptglqtd edstrrpcng
      121 drpqaakivr gpesvvavig shvrlgvhfv gtpaprvtwt kggkaavlpg drvdvsvdqp
      181 gscelrirqa ccddsgkyqv vvenslgsdf afasvkvegp pdppaeapra vslpsgviev
      241 twscspfdgg criqrylvda spasaqpraw srvaavlstc crlanlppaa sayvfvvraq
      301 narglsmasa esppaaplsp lgnddeeald ddviadpgpp vtlrpgeefc qdyhvheavg
      361 rgrfgvvhrc trladgtdaa akvvrcirtt erdrmrhela ilnllrghpr llrlldafek
      421 preiilvtey vsggelferv iaddfvltew dcvlflrqic agmaymhsrh vihldlkpen
      481 ilcqsqvghk iklidfglaq lyepssslrv mfgtpefvap evvsyepvsp atdmwsvgvi
      541 cyvllsglsp fmgdsdtdtf nnivrvdldf ddpvfetisa vakdfmcqli vknprkrmsa
      601 eqclehewlr vvakrgalpl ptdklkkfvi rrkwlksgna lkalsrmtnl sgqrpahgde
      661 aldllaceed apvsqpppsd asataaagee ad
//
LOCUS       XP_077501617             692 aa            linear   INV 25-NOV-2025
DEFINITION  myosin light chain kinase, smooth muscle-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501617
VERSION     XP_077501617.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645491.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..692
                     /product="myosin light chain kinase, smooth muscle-like
                     isoform X1"
                     /calculated_mol_wt=74467
     Region          127..217
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          143..147
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          156..160
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          183..187
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          197..202
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          210..213
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          359..609
                     /region_name="STKc_MLCK"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Myosin Light Chain Kinase; cd14103"
                     /db_xref="CDD:271005"
     Site            order(359..363,365,367,380,382,412,428..429,431,435,437,
                     475,477,479..480,482,494..495,498,513..516)
                     /site_type="active"
                     /db_xref="CDD:271005"
     Site            order(359..363,365,367,380,382,412,428..429,431,435,
                     479..480,482,494..495)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271005"
     Site            order(363,435,437,475,477,479,498,513..516)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271005"
     Site            order(494..502,505..516)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271005"
     CDS             1..692
                     /gene="LOC144112674"
                     /coded_by="XM_077645491.1:679..2757"
                     /db_xref="GeneID:144112674"
ORIGIN      
        1 maaaaasrcg adhkesfnpc qsasgqtfhp scgaspynae ttdsaavpsr qasqdtvsqe
       61 shapcavacp lqpesshnks rdpheivtgn iqqcaaalgt adsptglqtd edstrrpcng
      121 drpqaakivr gpesvvavig shvrlgvhfv gtpaprvtwt kggkaavlpg drvdvsvdqp
      181 gscelrirqa ccddsgkyqv vvenslgsdf afasvkvegp pdppaeapra vslpsgviev
      241 twscspfdgg criqrylvda spasaqpraw srvaavlstc crlanlppaa sayvfvvraq
      301 narglsmasa esppaaplsp lgnddeeald ddviadpgpp vtlrpgeefc qdyhvheavg
      361 rgrfgvvhrc trladgtdaa akvvrcirtt erdrmrhela ilnllrghpr llrlldafek
      421 preiilvtey vsggelferv iaddfvltew dcvlflrqic agmaymhsrh vihldlkpen
      481 ilcqsqvghk iklidfglaq lyepssslrv mfgtpefvap evvsyepvsp atdmwsvgvi
      541 cyvllsglsp fmgdsdtdtf nnivrvdldf ddpvfetisa vakdfmcqli vknprkrmsa
      601 eqclehewlr vvakrgalpl ptdklkkfvi rrkwlksgna lkalsrmtnl sgqrpahgde
      661 aldllaceed apvsqpppsd asataaagee ad
//
LOCUS       XP_077501618             476 aa            linear   INV 25-NOV-2025
DEFINITION  myosin light chain kinase, smooth muscle-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501618
VERSION     XP_077501618.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645492.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..476
                     /product="myosin light chain kinase, smooth muscle-like
                     isoform X2"
                     /calculated_mol_wt=52116
     Region          143..393
                     /region_name="STKc_MLCK"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Myosin Light Chain Kinase; cd14103"
                     /db_xref="CDD:271005"
     Site            order(143..147,149,151,164,166,196,212..213,215,219,221,
                     259,261,263..264,266,278..279,282,297..300)
                     /site_type="active"
                     /db_xref="CDD:271005"
     Site            order(143..147,149,151,164,166,196,212..213,215,219,
                     263..264,266,278..279)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271005"
     Site            order(147,219,221,259,261,263,282,297..300)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271005"
     Site            order(278..286,289..300)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271005"
     CDS             1..476
                     /gene="LOC144112674"
                     /coded_by="XM_077645492.1:148..1578"
                     /db_xref="GeneID:144112674"
ORIGIN      
        1 marppdppae apravslpsg vievtwscsp fdggcriqry lvdaspasaq prawsrvaav
       61 lstccrlanl ppaasayvfv vraqnargls masaesppaa plsplgndde ealdddviad
      121 pgppvtlrpg eefcqdyhvh eavgrgrfgv vhrctrladg tdaaakvvrc irtterdrmr
      181 helailnllr ghprllrlld afekpreiil vteyvsggel ferviaddfv ltewdcvlfl
      241 rqicagmaym hsrhvihldl kpenilcqsq vghkiklidf glaqlyepss slrvmfgtpe
      301 fvapevvsye pvspatdmws vgvicyvlls glspfmgdsd tdtfnnivrv dldfddpvfe
      361 tisavakdfm cqlivknprk rmsaeqcleh ewlrvvakrg alplptdklk kfvirrkwlk
      421 sgnalkalsr mtnlsgqrpa hgdealdlla ceedapvsqp ppsdasataa ageead
//
LOCUS       XP_077501619             487 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112676 [Amblyomma americanum].
ACCESSION   XP_077501619
VERSION     XP_077501619.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645493.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..487
                     /product="uncharacterized protein LOC144112676"
                     /calculated_mol_wt=52560
     CDS             1..487
                     /gene="LOC144112676"
                     /coded_by="XM_077645493.1:309..1772"
                     /db_xref="GeneID:144112676"
ORIGIN      
        1 mpllrlsaar alrendslrf nsccpfcifv nssaacgfvr epamerrtgq kyrtkyaygk
       61 rkrkppipkq krpaatephe rrsapissha dvsseaslpl esatssesgg lapsashdia
      121 vqyrpghssv faaasggavp vqrddgevlp kqkhtvqvhl psesitgses vgpapstsrd
      181 iavqyrpghs svfaagsaga vpvqrddgev fpkqkrtvqv hlpsesmtgs esgdpapsts
      241 rdiavqhrpg qssvfaaasd dampaqrdds elfpkqkrtv qvhleprfvt leaaeeqacs
      301 vratlravss terkfgfarq teeesapded eftliqasvm nsmigsllcp kcskkrlslk
      361 hetklglavk lvlscgacgp iashwsskrk segrtfevni ramqaiktig kgptalndfw
      421 atmnvshrgl hqktyqghlk klfkpgaeea aqnifadavl avkdvyskmq psspsnitvv
      481 ydgtwlt
//
LOCUS       XP_077501621             353 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112677 [Amblyomma americanum].
ACCESSION   XP_077501621
VERSION     XP_077501621.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645495.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..353
                     /product="uncharacterized protein LOC144112677"
                     /calculated_mol_wt=39499
     Region          107..>143
                     /region_name="FH_FOX"
                     /note="Forkhead (FH) domain found in Forkhead box (FOX)
                     family of transcription factors and similar proteins;
                     cl00061"
                     /db_xref="CDD:469596"
     CDS             1..353
                     /gene="LOC144112677"
                     /coded_by="XM_077645495.1:179..1240"
                     /db_xref="GeneID:144112677"
ORIGIN      
        1 mssrltaqfp eqssalpevq gsgrgmvpgg ssvgpirsaa clhstreagr hhvcqgffra
       61 rqafpphphh vqprrhvhha patsgspgra apcwhhrpqa sgqgklvqlv aqaihespik
      121 alrvrhvyea lkkrypyfmf mdkkelttwe gsesrpssrl ssssqsllvh tplpsfigsp
      181 grqlghmepq lhplvewelk terfatppsp siylnmeqer kpkpvyswsr gcydlyghqs
      241 qgysdevsta adtqllnepl pslpylcspr lthdtpvept srsyghpsph qepqlpltle
      301 qhsrpelsfa rqqiqpeqea mmprqqflpr pelsnsregt lphpelplpp qfp
//
LOCUS       XP_077501622             353 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112677 [Amblyomma americanum].
ACCESSION   XP_077501622
VERSION     XP_077501622.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645496.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..353
                     /product="uncharacterized protein LOC144112677"
                     /calculated_mol_wt=39499
     Region          107..>143
                     /region_name="FH_FOX"
                     /note="Forkhead (FH) domain found in Forkhead box (FOX)
                     family of transcription factors and similar proteins;
                     cl00061"
                     /db_xref="CDD:469596"
     CDS             1..353
                     /gene="LOC144112677"
                     /coded_by="XM_077645496.1:240..1301"
                     /db_xref="GeneID:144112677"
ORIGIN      
        1 mssrltaqfp eqssalpevq gsgrgmvpgg ssvgpirsaa clhstreagr hhvcqgffra
       61 rqafpphphh vqprrhvhha patsgspgra apcwhhrpqa sgqgklvqlv aqaihespik
      121 alrvrhvyea lkkrypyfmf mdkkelttwe gsesrpssrl ssssqsllvh tplpsfigsp
      181 grqlghmepq lhplvewelk terfatppsp siylnmeqer kpkpvyswsr gcydlyghqs
      241 qgysdevsta adtqllnepl pslpylcspr lthdtpvept srsyghpsph qepqlpltle
      301 qhsrpelsfa rqqiqpeqea mmprqqflpr pelsnsregt lphpelplpp qfp
//
LOCUS       XP_077501623             231 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112678 [Amblyomma americanum].
ACCESSION   XP_077501623
VERSION     XP_077501623.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645497.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..231
                     /product="uncharacterized protein LOC144112678"
                     /calculated_mol_wt=26045
     CDS             1..231
                     /gene="LOC144112678"
                     /coded_by="XM_077645497.1:515..1210"
                     /db_xref="GeneID:144112678"
ORIGIN      
        1 mfmdkkeltt wegsesrpss rlssssqsll vhtplpsfig spgrqlghme pqlhplvewe
       61 lkterfatpp spsiylnmeq erkpkpvysw srgcydlygh qsqgysdevs taadtqllne
      121 plpslpylcs prlthdtpve ptsrsyghps phqepqlplt leqhsrpels farqqiqpeq
      181 eammprqqfl prpelsnsre gtlphpelpl ppqfpwppqg pslprgptlp p
//
LOCUS       XP_077501624             538 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112680 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501624
VERSION     XP_077501624.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645498.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..538
                     /product="uncharacterized protein LOC144112680 isoform X1"
                     /calculated_mol_wt=58979
     CDS             1..538
                     /gene="LOC144112680"
                     /coded_by="XM_077645498.1:684..2300"
                     /db_xref="GeneID:144112680"
ORIGIN      
        1 mgvpvmtdmc qggsshastp cmevgvtesl galqtgfggn yedcdegrgl vpkavifctq
       61 trqeglsgad skkevafral qkrccmvrde rsnrtegcsg nmvrlggkms lhfdgavtti
      121 vlasaiafvd lpllvflgvm mrhrqsalpl ltdlkvpali vieslifagt evvlylclea
      181 lhkgrhsasf avlvltrlly tvwlavlfra sgpllasgla mmrdrfamaa flyaydgysk
      241 ylvclprtls hlygsetctl ptwcwtpsgr skedifeaca ylyemlamse haeviqihhe
      301 kydphmrnfv rdntlgacvw vcalslavlv ilgmlkmiwg eiaavvhlyk rytaerrkgv
      361 trndafhava lallptdtsn meplltgkeq rckvisilgp itasravsah nvvhnqkcpd
      421 vnpavapevv dlhgapessd alhpsldnas qshvlvefrq tlkpqgapqh crrftgfdan
      481 iflndaedpf msrhqigrac vvrivssvtk raalatrlal rlvmfrmtts sasrplqf
//
LOCUS       XP_077501625             473 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112680 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501625
VERSION     XP_077501625.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645499.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..473
                     /product="uncharacterized protein LOC144112680 isoform X2"
                     /calculated_mol_wt=52033
     CDS             1..473
                     /gene="LOC144112680"
                     /coded_by="XM_077645499.1:683..2104"
                     /db_xref="GeneID:144112680"
ORIGIN      
        1 mgvpvmtdmc qggsshastp cmevgvtesl galqtgfggn yedcdegrgl vpkavifctq
       61 trqeglsgad skkevafral qkrccmvrde rsnrtegcsg nmvrlggkms lhfdgavtti
      121 vlasaiafvd lpllvflgvm mrhrqsalpl ltdlkmmrdr famaaflyay dgyskylvcl
      181 prtlshlygs etctlptwcw tpsgrskedi feacaylyem lamsehaevi qihhekydph
      241 mrnfvrdntl gacvwvcals lavlvilgml kmiwgeiaav vhlykrytae rrkgvtrnda
      301 fhavalallp tdtsnmepll tgkeqrckvi silgpitasr avsahnvvhn qkcpdvnpav
      361 apevvdlhga pessdalhps ldnasqshvl vefrqtlkpq gapqhcrrft gfdaniflnd
      421 aedpfmsrhq igracvvriv ssvtkraala trlalrlvmf rmttssasrp lqf
//
LOCUS       XP_077501626             453 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112680 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501626
VERSION     XP_077501626.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645500.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..453
                     /product="uncharacterized protein LOC144112680 isoform X3"
                     /calculated_mol_wt=50062
     CDS             1..453
                     /gene="LOC144112680"
                     /coded_by="XM_077645500.1:728..2089"
                     /db_xref="GeneID:144112680"
ORIGIN      
        1 mvrdersnrt egcsgnmvrl ggkmslhfdg avttivlasa iafvdlpllv flgvmmrhrq
       61 salplltdlk vpaliviesl ifagtevvly lclealhkgr hsasfavlvl trllytvwla
      121 vlfrasgpll asglammrdr famaaflyay dgyskylvcl prtlshlygs etctlptwcw
      181 tpsgrskedi feacaylyem lamsehaevi qihhekydph mrnfvrdntl gacvwvcals
      241 lavlvilgml kmiwgeiaav vhlykrytae rrkgvtrnda fhavalallp tdtsnmepll
      301 tgkeqrckvi silgpitasr avsahnvvhn qkcpdvnpav apevvdlhga pessdalhps
      361 ldnasqshvl vefrqtlkpq gapqhcrrft gfdaniflnd aedpfmsrhq igracvvriv
      421 ssvtkraala trlalrlvmf rmttssasrp lqf
//
LOCUS       XP_077501627             453 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112680 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501627
VERSION     XP_077501627.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645501.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..453
                     /product="uncharacterized protein LOC144112680 isoform X3"
                     /calculated_mol_wt=50062
     CDS             1..453
                     /gene="LOC144112680"
                     /coded_by="XM_077645501.1:605..1966"
                     /db_xref="GeneID:144112680"
ORIGIN      
        1 mvrdersnrt egcsgnmvrl ggkmslhfdg avttivlasa iafvdlpllv flgvmmrhrq
       61 salplltdlk vpaliviesl ifagtevvly lclealhkgr hsasfavlvl trllytvwla
      121 vlfrasgpll asglammrdr famaaflyay dgyskylvcl prtlshlygs etctlptwcw
      181 tpsgrskedi feacaylyem lamsehaevi qihhekydph mrnfvrdntl gacvwvcals
      241 lavlvilgml kmiwgeiaav vhlykrytae rrkgvtrnda fhavalallp tdtsnmepll
      301 tgkeqrckvi silgpitasr avsahnvvhn qkcpdvnpav apevvdlhga pessdalhps
      361 ldnasqshvl vefrqtlkpq gapqhcrrft gfdaniflnd aedpfmsrhq igracvvriv
      421 ssvtkraala trlalrlvmf rmttssasrp lqf
//
LOCUS       XP_077501628             137 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112682 [Amblyomma americanum].
ACCESSION   XP_077501628
VERSION     XP_077501628.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645502.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..137
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..137
                     /product="uncharacterized protein LOC144112682"
                     /calculated_mol_wt=15506
     Region          <1..134
                     /region_name="TBP_TLF"
                     /note="TATA box binding protein (TBP): Present in archaea
                     and eukaryotes, TBPs are transcription factors that
                     recognize promoters and initiate transcription. TBP has
                     been shown to be an essential component of three different
                     transcription initiation complexes:...; cl08263"
                     /db_xref="CDD:447593"
     Site            order(68..69,81..85,88,93)
                     /site_type="other"
                     /note="TFIIB interaction surface [polypeptide binding]"
                     /db_xref="CDD:238352"
     Site            order(92..93,98,129..130)
                     /site_type="other"
                     /note="NC2 interaction surface [polypeptide binding]"
                     /db_xref="CDD:238352"
     CDS             1..137
                     /gene="LOC144112682"
                     /coded_by="XM_077645502.1:57..470"
                     /db_xref="GeneID:144112682"
ORIGIN      
        1 mrireprtta llfrsgkivc tgarselqas iaarkfarit qklrynarft dfkvqnivas
       61 cdvgfrikle slaliesqcs cyepelfpgl vyrmvqpsvl llvfvsgkvv ltgakvrehi
      121 yqafdkiypi lqnhrkv
//
LOCUS       XP_077501630             263 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112684 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501630
VERSION     XP_077501630.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645504.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..263
                     /product="uncharacterized protein LOC144112684 isoform X1"
                     /calculated_mol_wt=30134
     Region          143..>179
                     /region_name="FH_FOX"
                     /note="Forkhead (FH) domain found in Forkhead box (FOX)
                     family of transcription factors and similar proteins;
                     cl00061"
                     /db_xref="CDD:469596"
     CDS             1..263
                     /gene="LOC144112684"
                     /coded_by="XM_077645504.1:221..1012"
                     /db_xref="GeneID:144112684"
ORIGIN      
        1 mnnvnyspsk rrqnivallf ngdiyhrkkg smlqvnncrl lsdrrhtema teecypwkpa
       61 yfsnetwckl pddirkcygn vdkdrsiker kassepakrs pphphhvqpr rhvhhapats
      121 gspgraapcw hhcpqasgqg klvqlvaqai hespikalrv rhvyealkkr ypyfmfmdkk
      181 elttwegses rpssrlssss qslfvhtplp sfigspgrql ghmepqlhpl vewelkterf
      241 atppspsiyl nteqerkpkp vys
//
LOCUS       XP_077501631             263 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112684 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501631
VERSION     XP_077501631.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645505.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..263
                     /product="uncharacterized protein LOC144112684 isoform X1"
                     /calculated_mol_wt=30134
     Region          143..>179
                     /region_name="FH_FOX"
                     /note="Forkhead (FH) domain found in Forkhead box (FOX)
                     family of transcription factors and similar proteins;
                     cl00061"
                     /db_xref="CDD:469596"
     CDS             1..263
                     /gene="LOC144112684"
                     /coded_by="XM_077645505.1:397..1188"
                     /db_xref="GeneID:144112684"
ORIGIN      
        1 mnnvnyspsk rrqnivallf ngdiyhrkkg smlqvnncrl lsdrrhtema teecypwkpa
       61 yfsnetwckl pddirkcygn vdkdrsiker kassepakrs pphphhvqpr rhvhhapats
      121 gspgraapcw hhcpqasgqg klvqlvaqai hespikalrv rhvyealkkr ypyfmfmdkk
      181 elttwegses rpssrlssss qslfvhtplp sfigspgrql ghmepqlhpl vewelkterf
      241 atppspsiyl nteqerkpkp vys
//
LOCUS       XP_077501632             262 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112684 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501632
VERSION     XP_077501632.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645506.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..262
                     /product="uncharacterized protein LOC144112684 isoform X2"
                     /calculated_mol_wt=30064
     Region          142..>178
                     /region_name="FH_FOX"
                     /note="Forkhead (FH) domain found in Forkhead box (FOX)
                     family of transcription factors and similar proteins;
                     cl00061"
                     /db_xref="CDD:469596"
     CDS             1..262
                     /gene="LOC144112684"
                     /coded_by="XM_077645506.1:169..957"
                     /db_xref="GeneID:144112684"
ORIGIN      
        1 mrrvlrgrrp ptraqpldeq rellaqqapt khrglalqwr hlppkeemat eecypwkpay
       61 fsnetwcklp ddirkcygnv dkdrsikerk assepakrsp phphhvqprr hvhhapatsg
      121 spgraapcwh hcpqasgqgk lvqlvaqaih espikalrvr hvyealkkry pyfmfmdkke
      181 lttwegsesr pssrlssssq slfvhtplps figspgrqlg hmepqlhplv ewelkterfa
      241 tppspsiyln teqerkpkpv ys
//
LOCUS       XP_077501633             215 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112684 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501633
VERSION     XP_077501633.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645507.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..215
                     /product="uncharacterized protein LOC144112684 isoform X3"
                     /calculated_mol_wt=24464
     Region          95..>131
                     /region_name="FH_FOX"
                     /note="Forkhead (FH) domain found in Forkhead box (FOX)
                     family of transcription factors and similar proteins;
                     cl00061"
                     /db_xref="CDD:469596"
     CDS             1..215
                     /gene="LOC144112684"
                     /coded_by="XM_077645507.1:936..1583"
                     /db_xref="GeneID:144112684"
ORIGIN      
        1 mateecypwk payfsnetwc klpddirkcy gnvdkdrsik erkassepak rspphphhvq
       61 prrhvhhapa tsgspgraap cwhhcpqasg qgklvqlvaq aihespikal rvrhvyealk
      121 krypyfmfmd kkelttwegs esrpssrlss ssqslfvhtp lpsfigspgr qlghmepqlh
      181 plvewelkte rfatppspsi ylnteqerkp kpvys
//
LOCUS       XP_077501634             472 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112685 [Amblyomma americanum].
ACCESSION   XP_077501634
VERSION     XP_077501634.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645508.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..472
                     /product="uncharacterized protein LOC144112685"
                     /calculated_mol_wt=52378
     Region          221..267
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          302..462
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..472
                     /gene="LOC144112685"
                     /coded_by="XM_077645508.1:158..1576"
                     /db_xref="GeneID:144112685"
ORIGIN      
        1 madgsewvpn ertricskhf vngekstias hpgyyptifp dayrvshvda tkqlaryerl
       61 hrrrrarass asvipkqasa tvsekdtass teedtsetdp lnqepsnlev lcmavdaaqk
      121 descqtddia rcsgtfsvfl csargneact qvnhkivrda evqckpavvs rqsgrdsrta
      181 cftgydsvqa caaamkdlcg vspdiyalll simprsaert cdvpitdrlv lflmklklgi
      241 tysslavlfc vsrntvsrhf ksvlktlsia tqkwiyrppd hvirqtmpnc fkvhyqdcsm
      301 iidctevrie qpytvqqqrv lyshykggyt lkflvgitpc gavcfcskay ggrcsdsfit
      361 vdsgfldlvq pgdvvmadkg fpgikaglee vravlvmppf lhgqdqfsgs evhetyqiaq
      421 vrihveriiq rikmfnilnt ripteliavm sdvfhvccvl anlqppiiss eq
//
LOCUS       XP_077501635             451 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112686 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501635
VERSION     XP_077501635.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645509.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..451
                     /product="uncharacterized protein LOC144112686 isoform X1"
                     /calculated_mol_wt=50790
     Region          16..>140
                     /region_name="SWIM"
                     /note="Uncharacterized protein, contains SWIM-type Zn
                     finger domain [Function unknown]; COG4715"
                     /db_xref="CDD:443750"
     Region          261..441
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(334,368,381,383)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..451
                     /gene="LOC144112686"
                     /coded_by="XM_077645509.1:108..1463"
                     /db_xref="GeneID:144112686"
ORIGIN      
        1 msqvvlagfr pikgteeyfh pasltsgkkl ydaghvsfvt evdgvnvrak crsqqgkqvy
       61 dvslhinknd relqaglcsc rygaagsckh caavalhvnm hddisctser qrwgrpskka
      121 rvddkasiee llgankhphv gggeppkpld psflikhfpd iqcpmsrilk aqnendesac
      181 ktvlqdiidk vlleeeiekl rsllqepkka lhslmvtakf pkilikkkey eeempaevaa
      241 yyaknikcls alhlcaltkg qannhrwhre rrlritcsqa hmilrtrkpp qdllktilhr
      301 rgfcseatsy gtfsnittsh vgltesyfsg mkmepvarkn feekvcadvl etglvvhtaq
      361 pwlcgspdgl fetakgvcll eikcpfsrrn rdiidvdqei tfvpyikykn gkltllashr
      421 yytqvqllmy vcnlnecffl filnnkasps l
//
LOCUS       XP_077501636             432 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112686 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501636
VERSION     XP_077501636.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645510.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..432
                     /product="uncharacterized protein LOC144112686 isoform X2"
                     /calculated_mol_wt=48760
     Region          16..>140
                     /region_name="SWIM"
                     /note="Uncharacterized protein, contains SWIM-type Zn
                     finger domain [Function unknown]; COG4715"
                     /db_xref="CDD:443750"
     Region          261..420
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(315,349,362,364)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..432
                     /gene="LOC144112686"
                     /coded_by="XM_077645510.1:108..1406"
                     /db_xref="GeneID:144112686"
ORIGIN      
        1 msqvvlagfr pikgteeyfh pasltsgkkl ydaghvsfvt evdgvnvrak crsqqgkqvy
       61 dvslhinknd relqaglcsc rygaagsckh caavalhvnm hddisctser qrwgrpskka
      121 rvddkasiee llgankhphv gggeppkpld psflikhfpd iqcpmsrilk aqnendesac
      181 ktvlqdiidk vlleeeiekl rsllqepkka lhslmvtakf pkilikkkey eeempaevaa
      241 yyaknikcls alhlcaltkg qannhrwhre rrlritcsqa hmilrtrkpp qdllktilhr
      301 rgfcseatsy gmkmepvark nfeekvcadv letglvvhta qpwlcgspdg lfetakgvcl
      361 leikcpfsrr nrdiidvdqe itfvpyikyk ngkltllash ryytqvqllm yvcnlnecff
      421 lfilnnkasp sl
//
LOCUS       XP_077501637             352 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112686 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501637
VERSION     XP_077501637.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645511.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..352
                     /product="uncharacterized protein LOC144112686 isoform X3"
                     /calculated_mol_wt=40084
     Region          162..342
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(235,269,282,284)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..352
                     /gene="LOC144112686"
                     /coded_by="XM_077645511.1:364..1422"
                     /db_xref="GeneID:144112686"
ORIGIN      
        1 mhddisctse rqrwgrpskk arvddkasie ellgankhph vgggeppkpl dpsflikhfp
       61 diqcpmsril kaqnendesa cktvlqdiid kvlleeeiek lrsllqepkk alhslmvtak
      121 fpkilikkke yeeempaeva ayyaknikcl salhlcaltk gqannhrwhr errlritcsq
      181 ahmilrtrkp pqdllktilh rrgfcseats ygtfsnitts hvgltesyfs gmkmepvark
      241 nfeekvcadv letglvvhta qpwlcgspdg lfetakgvcl leikcpfsrr nrdiidvdqe
      301 itfvpyikyk ngkltllash ryytqvqllm yvcnlnecff lfilnnkasp sl
//
LOCUS       XP_077501638            1324 aa            linear   INV 25-NOV-2025
DEFINITION  anion exchange protein Ae2 [Amblyomma americanum].
ACCESSION   XP_077501638
VERSION     XP_077501638.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645512.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1324
                     /product="anion exchange protein Ae2"
                     /calculated_mol_wt=148261
     Region          411..1323
                     /region_name="Band_3_cyto"
                     /note="Band 3 cytoplasmic domain; cl26877"
                     /db_xref="CDD:452680"
     CDS             1..1324
                     /gene="Ae2"
                     /coded_by="XM_077645512.1:271..4245"
                     /db_xref="GeneID:144112688"
ORIGIN      
        1 megrrssqlw kdegsvleai demmapssdf slsdiqdkqe akstkehqyd eedykmhrga
       61 gfphhhqplk khhyqrrrss apntagnqds pdlpavaegk epsnsskdse edgdeaisvk
      121 kvddtedgka skqpeetaed epgpsglkps vpaktgkpag vrepprifvt sdtepevefl
      181 paqlkpkasd aslsekplks slkkssispd dqplgsygff pnldtrtaey grqyadvpls
      241 staressqeq nesdgaqhkv afilgsdste easppqpavg dlqpsstskg vepfrakdrh
      301 hhhhhrhkrr ryiydpslmv lhdaedkhrv vtepeeamtl qsadleemas hrfedqkgmr
      361 rhkilakntv lsfmqpased ssekpsapgs gkrysvpfvk klydrkphei fveldelvpe
      421 tyewketarw ikyeenlked vdrwgkphla slsfhsllcl rkcieqgtvl ldleekdltg
      481 iankvvdnmv lteqippesh gavlrvlllr hrhgaeksln tflrrnssgt ftalkslrsa
      541 slvsnftsss qdhvngnagn rsmplltadt risidkgtrd etepflpgcs tddlrkpdan
      601 ilrripedae atavlvggld fleqptiafv rlaqgqvmpn lmevpipvrf ffillgptnv
      661 ddldyhevgr sistlmsnrt fhtaaykaed rrdllraine flddsivlpp gdwdrksllp
      721 vddlkkkyed irkrkmqakk pappaedklp qyeplkrsgr lfgglcsdik nrypwylsdf
      781 kdaldgqcla taifiyfaal sgaitfggll gdktnnligv setlvatcat giifalfsgq
      841 plviigvtgp vllfdellya fceendvefl airvwigmwi ailatlvvaf egsalvrfft
      901 rftqeifasl isllyiyesf yklylifvth pllrandycd ndnateaylp snisatnwtn
      961 astanwsgel npvfeiiert rqpntallsm ilmfstffla dflrkfrnss flgrnarral
     1021 gdfgipiaii aivtldffvp ntysqklkvp nglstsiegr gwliapisst rpfiwwhifv
     1081 aalgallvfi llfleieice lilskkerkl qkgsgfhldl llicymelgc aflgapwmca
     1141 atvrsvshla sltvmsrtha pgesphiigv keqrvtnllv allvglsvfm spllrevpva
     1201 vlfgvflymg itsmigiqlf eriilffkpt khfpsvpyaq kvkatkmhly tllqivcliv
     1261 lwavksssla lafpfvlllm iplrlqlkyi ftekelqcld gedvnlqsde eddpdfyqqt
     1321 llps
//
LOCUS       XP_077501639            1324 aa            linear   INV 25-NOV-2025
DEFINITION  anion exchange protein Ae2 [Amblyomma americanum].
ACCESSION   XP_077501639
VERSION     XP_077501639.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645513.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1324
                     /product="anion exchange protein Ae2"
                     /calculated_mol_wt=148261
     Region          411..1323
                     /region_name="Band_3_cyto"
                     /note="Band 3 cytoplasmic domain; cl26877"
                     /db_xref="CDD:452680"
     CDS             1..1324
                     /gene="Ae2"
                     /coded_by="XM_077645513.1:184..4158"
                     /db_xref="GeneID:144112688"
ORIGIN      
        1 megrrssqlw kdegsvleai demmapssdf slsdiqdkqe akstkehqyd eedykmhrga
       61 gfphhhqplk khhyqrrrss apntagnqds pdlpavaegk epsnsskdse edgdeaisvk
      121 kvddtedgka skqpeetaed epgpsglkps vpaktgkpag vrepprifvt sdtepevefl
      181 paqlkpkasd aslsekplks slkkssispd dqplgsygff pnldtrtaey grqyadvpls
      241 staressqeq nesdgaqhkv afilgsdste easppqpavg dlqpsstskg vepfrakdrh
      301 hhhhhrhkrr ryiydpslmv lhdaedkhrv vtepeeamtl qsadleemas hrfedqkgmr
      361 rhkilakntv lsfmqpased ssekpsapgs gkrysvpfvk klydrkphei fveldelvpe
      421 tyewketarw ikyeenlked vdrwgkphla slsfhsllcl rkcieqgtvl ldleekdltg
      481 iankvvdnmv lteqippesh gavlrvlllr hrhgaeksln tflrrnssgt ftalkslrsa
      541 slvsnftsss qdhvngnagn rsmplltadt risidkgtrd etepflpgcs tddlrkpdan
      601 ilrripedae atavlvggld fleqptiafv rlaqgqvmpn lmevpipvrf ffillgptnv
      661 ddldyhevgr sistlmsnrt fhtaaykaed rrdllraine flddsivlpp gdwdrksllp
      721 vddlkkkyed irkrkmqakk pappaedklp qyeplkrsgr lfgglcsdik nrypwylsdf
      781 kdaldgqcla taifiyfaal sgaitfggll gdktnnligv setlvatcat giifalfsgq
      841 plviigvtgp vllfdellya fceendvefl airvwigmwi ailatlvvaf egsalvrfft
      901 rftqeifasl isllyiyesf yklylifvth pllrandycd ndnateaylp snisatnwtn
      961 astanwsgel npvfeiiert rqpntallsm ilmfstffla dflrkfrnss flgrnarral
     1021 gdfgipiaii aivtldffvp ntysqklkvp nglstsiegr gwliapisst rpfiwwhifv
     1081 aalgallvfi llfleieice lilskkerkl qkgsgfhldl llicymelgc aflgapwmca
     1141 atvrsvshla sltvmsrtha pgesphiigv keqrvtnllv allvglsvfm spllrevpva
     1201 vlfgvflymg itsmigiqlf eriilffkpt khfpsvpyaq kvkatkmhly tllqivcliv
     1261 lwavksssla lafpfvlllm iplrlqlkyi ftekelqcld gedvnlqsde eddpdfyqqt
     1321 llps
//
LOCUS       XP_077501640             355 aa            linear   INV 25-NOV-2025
DEFINITION  Golgi reassembly-stacking protein 2 [Amblyomma americanum].
ACCESSION   XP_077501640
VERSION     XP_077501640.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645514.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..355
                     /product="Golgi reassembly-stacking protein 2"
                     /calculated_mol_wt=36820
     Region          19..321
                     /region_name="GRH1"
                     /note="Peripheral Golgi membrane protein [Intracellular
                     trafficking and secretion]; COG5233"
                     /db_xref="CDD:227558"
     Region          69..204
                     /region_name="GRASP55_65"
                     /note="GRASP55/65 PDZ-like domain; pfam04495"
                     /db_xref="CDD:427981"
     CDS             1..355
                     /gene="Grasp65"
                     /coded_by="XM_077645514.1:118..1185"
                     /db_xref="GeneID:144112689"
ORIGIN      
        1 mggsqssaip gggtegyhvl rvqenspghk agleaffdfi vaigntrlnq dndvlkdvlk
       61 aniekplvmt vyssktqtvr dveivpssmw gghgllgvsi rfcsfegane nvwhvldvsp
      121 hspaeaaglr pyadyiigsd svlhesedlf tlidahegrp lklyvyntdt dgcrevtitp
      181 nkswggdgcl gcgigygylh riptkpvakt stwpasnapa gdgfssvqls apslvgdpaa
      241 tlpqtlnals lndastqpta espvlasrep apsvaaevae srppasapfg seqdrtgaan
      301 avplttpinl pgmppitvsa slpadalasl gtssevsglr eeasaksama apqhl
//
LOCUS       XP_077501641             557 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier family 2, facilitated glucose transporter member
            8-like [Amblyomma americanum].
ACCESSION   XP_077501641
VERSION     XP_077501641.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645515.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..557
                     /product="solute carrier family 2, facilitated glucose
                     transporter member 8-like"
                     /calculated_mol_wt=59072
     Region          16..452
                     /region_name="MFS_GLUT6_8_Class3_like"
                     /note="Glucose transporter (GLUT) types 6 and 8, Class 3
                     GLUTs, and similar transporters of the Major Facilitator
                     Superfamily; cd17358"
                     /db_xref="CDD:340916"
     Site            order(29,33,152,155..156,159,265..266,270..271,274..275,
                     301,368..369,372..373,377,400..401,404)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:340916"
     Region          <480..548
                     /region_name="PTZ00436"
                     /note="60S ribosomal protein L19-like protein;
                     Provisional"
                     /db_xref="CDD:185616"
     CDS             1..557
                     /gene="LOC144112690"
                     /coded_by="XM_077645515.1:139..1812"
                     /db_xref="GeneID:144112690"
ORIGIN      
        1 madkkedgpk gfelfyvaaa sawlgsvamg tnlgysspai pslknnvsqg gleiteseet
       61 wfgslmtlga ltgglvagfl vdslgrklsi ifsslgfitg wvliatassv ivlclgrvit
      121 gfftglvsla vpvyvseisr pqvrgtlgtg iqlsvtigil avfffgkylg wsslailclt
      181 vpagmavlmi fmsesprwll qkgkredalk alqflygagt dyeaernqie lnlkmspteg
      241 fhmkelqqpf iykpilislf lmfaqqlsgi navmfyavsi fqsagttipp edcmviigvv
      301 qvvatlgatm vmdkggrrvl lltsaallal slgvlggyhy vkstkgdeav etigwlplvc
      361 lslfiigfsc gmgpipwlmm gellplrvrg fatgictsfn wtmafivtkt fndmldilkp
      421 ygtywffcav miisffvvvl tlpetkgktl eeieaafrgv avpepkkpep apaevspsip
      481 assaappads gkppaaaspk rpsvpsthsk sskatspdka aisptrqask ttvseappse
      541 papkspaqeq dgdgggn
//
LOCUS       XP_077501643             496 aa            linear   INV 25-NOV-2025
DEFINITION  cell division cycle 6 [Amblyomma americanum].
ACCESSION   XP_077501643
VERSION     XP_077501643.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645517.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..496
                     /product="cell division cycle 6"
                     /calculated_mol_wt=54451
     Region          114..>336
                     /region_name="CDC6"
                     /note="Cdc6-related protein, AAA superfamily ATPase
                     [Replication, recombination and repair]; COG1474"
                     /db_xref="CDD:441083"
     Region          411..479
                     /region_name="Cdc6_C"
                     /note="CDC6, C terminal winged helix domain; pfam09079"
                     /db_xref="CDD:462672"
     CDS             1..496
                     /gene="Cdc6"
                     /coded_by="XM_077645517.1:253..1743"
                     /db_xref="GeneID:144112691"
ORIGIN      
        1 mpytqsvlql pvrkssrcaa ktqqsrrsps sskkavfden sasqvhrvsk rlfsgddaac
       61 ssdnaahlse asqspkkpka issqenlvpl cvsgvdtsly qkakqsltsq ssvrvvgrqq
      121 emetmkdflh fhlrsrssss lyisgapgtg ktaclsqiil sakdlftfkf vfvncmtlqt
      181 ssaiyekivt glgfsasgsn lldtiraklt tkghpmvivm deidqldskn qtvlyslfel
      241 pqlknsrvil fgianaldlt drvlphlqay gcqpmllhfa pysreeivai ltdrlsecha
      301 vihpqaiqfc arkiaactgd vrkaldicrr avevvegstk tqqvlvprpe hgynlgspkk
      361 aplkvvsmah issvladvfg ssamgskdln atlplqqkll lctlvvllkv rklrdvplgk
      421 lqevymricq khqcstdqsd fqslstlles rgliavkkhk elrlckiclr vdekeveyvl
      481 qdkallaacl ndsavl
//
LOCUS       XP_077501644             883 aa            linear   INV 25-NOV-2025
DEFINITION  ATP-dependent DNA helicase DDX11 [Amblyomma americanum].
ACCESSION   XP_077501644
VERSION     XP_077501644.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645518.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..883
                     /product="ATP-dependent DNA helicase DDX11"
                     /calculated_mol_wt=98315
     Region          32..>64
                     /region_name="DEAD-like_helicase_N"
                     /note="N-terminal helicase domain of the DEAD-box helicase
                     superfamily; cl28899"
                     /db_xref="CDD:475120"
     Site            41..47
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:350670"
     Region          <57..>172
                     /region_name="PRK03918"
                     /note="DNA double-strand break repair ATPase Rad50"
                     /db_xref="CDD:235175"
     Region          195..855
                     /region_name="rad3"
                     /note="DNA repair helicase (rad3); TIGR00604"
                     /db_xref="CDD:273169"
     CDS             1..883
                     /gene="LOC144112692"
                     /coded_by="XM_077645518.1:121..2772"
                     /db_xref="GeneID:144112692"
ORIGIN      
        1 mdsdsvdfhf pfepypvqss fmkalyaaln gskvgifesp tgtgkslsli cgaltwlsey
       61 knskkatlea eladlqekia spaetgddwi knsaekmllr hrvrevqcqy dalmnkekky
      121 eqlkedvkkr rtrvqdsnar sgpeanddia ketesiarll aeineneedv vedyssdddt
      181 prddddlpdt vlepkiiycs rthsqltqfv relqktvfsd rvrtvalasr gnlcindavt
      241 klsslslind rcldmqkass kskasasper kraktksvgk cpfyrhqaie alaadvltev
      301 qdieqvvhha rkekacpyya sryatpdael vlipynillq kttreackld lrgsvviide
      361 ahnlletitd lhsvhlkmsa lkdaqrvlse yynryharmn tknvmyikqv lymlkrfilh
      421 ltgdgkgaiq edtstmcsvt afrinsgttn inlfkvvryl eqskvalkvn lfakkstvai
      481 seklestgaa klsdfihsmk kcqskkpsak emapqqpvns faalgnplit vheffrelay
      541 shfdgqvlvh kakepansfl kflllspanh faeiasevhs lilaggtmqp tsefvdqlli
      601 pagvapdrvm hfscghvvak enlsvialsh gpmgkvfeft yrnkmdpeii delgrvilnv
      661 trvvpggivc ffpsyeyeha vserwkvtgv leklsmkkhi fheplkssel estlaqysqc
      721 akavsssgpl tgailfsvvg gkmseginfs ddmgrcvvmv glpfpnakap emmskmeyls
      781 atypktndgh tagqvyyesv cmkavnqsig rairhkddya iillldqryq rhsimkalpa
      841 wiqgslttas kfgaafatiq qffrgrrnst qleasgkvgt gnp
//
LOCUS       XP_077501645             799 aa            linear   INV 25-NOV-2025
DEFINITION  transferrin 2 [Amblyomma americanum].
ACCESSION   XP_077501645
VERSION     XP_077501645.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645519.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..799
                     /product="transferrin 2"
                     /calculated_mol_wt=88810
     Region          28..318
                     /region_name="PBP2_transferrin"
                     /note="Transferrin family of the type 2
                     periplasmic-binding protein superfamily; cd13529"
                     /db_xref="CDD:270247"
     Site            order(84,113,139,146,280)
                     /site_type="active"
                     /db_xref="CDD:270247"
     Region          428..770
                     /region_name="PBP2_transferrin"
                     /note="Transferrin family of the type 2
                     periplasmic-binding protein superfamily; cd13529"
                     /db_xref="CDD:270247"
     Site            order(484,512,538,545,689)
                     /site_type="active"
                     /db_xref="CDD:270247"
     CDS             1..799
                     /gene="Tsf2"
                     /coded_by="XM_077645519.1:120..2519"
                     /db_xref="GeneID:144112693"
ORIGIN      
        1 mwsslrhlss fvviavfhaf pshgqqdvtw ctvsdaehlk ctefaeavrv srvfslalkc
       61 kraafkdqcm nfldngqanl veldpgevyt agrfhsvipi laerygadre agyysvavvh
      121 snatfhslnd lrnksvcfts vgdmagwvvp matlihenil evtdcnnlvk saasffgpsc
      181 apnslidkhn ptgdnpqkmc elctgrpger csgndpfsgy qgalrclmek gdvafvkhtt
      241 lyeaflgqpp sdrfrllcpt gqlampdqyr tcnwgmvppn vvvttsdtlp rvrvqyqefl
      301 qlavrtfgkp pigqlpwfyq shvdgdgynl nptpdwsrgf nqslpagqpt spwsglptte
      361 rsvivnvtel skfylfasgi rygnatnlll qdlttelval ngyeqtftgy lgthvdhftk
      421 lrrcpvppav lcvvaekeve kchrmktafk sqmlkpdlnc vrshshlgcm hmiqdgladl
      481 vvleagdiyr aghsfglipi iteqynldep yyyavavtlq gdketdllyl kgktschtgi
      541 nqaagwvvpl sflisnermr sygcdsarsa seffskscvp galsrefvgs ersyknlcdl
      601 chgtsthfcg rdasepfygh tgafrclveg ggqiafvkht tvfentagrn smwwarnimp
      661 gdfelvcrdg srrpqdkyeq cnlgkvasna ivtspnkpqs mideyinlfi yaqqfygsky
      721 sedftfkmfv seaahrdlif qdstqqlkpv plhrrnyady lgsdflqavr lvdcsagsrg
      781 aqlsvvvmlv calslnfwa
//
LOCUS       XP_077501646             282 aa            linear   INV 25-NOV-2025
DEFINITION  DDRGK domain-containing protein 1-like [Amblyomma americanum].
ACCESSION   XP_077501646
VERSION     XP_077501646.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645520.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..282
                     /product="DDRGK domain-containing protein 1-like"
                     /calculated_mol_wt=32390
     Region          90..277
                     /region_name="DDRGK"
                     /note="DDRGK domain; pfam09756"
                     /db_xref="CDD:370664"
     CDS             1..282
                     /gene="LOC144112694"
                     /coded_by="XM_077645520.1:140..988"
                     /db_xref="GeneID:144112694"
ORIGIN      
        1 msdlimiltg aislvtiili gllvwrgsms sdkgpqarav pinregggpa girrprrnar
       61 armgagrqld ddddeeddem ledmiampeg kigakkrkkl emkaekraqr erdledreer
      121 rqkeamdeer rkqeeqreke leaereaeek rlkeekerqe leeylkmkea fsvedegyda
      181 aneeqaqnkl qefldhikke kvviledlaa kfqlktqdci drvqallade tlvgviddrg
      241 kfiyitrqel eevahfirqr grvsiselvd ssnslinltp aa
//
LOCUS       XP_077501647             609 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112695 [Amblyomma americanum].
ACCESSION   XP_077501647
VERSION     XP_077501647.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645521.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..609
                     /product="uncharacterized protein LOC144112695"
                     /calculated_mol_wt=66576
     Region          507..596
                     /region_name="PWWP_PWWP2"
                     /note="PWWP domain found in the PWWP domain-containing
                     protein 2 (PWWP2) family; cd20140"
                     /db_xref="CDD:438968"
     Site            order(523,526,552)
                     /site_type="other"
                     /note="putative methyl-histone binding site [polypeptide
                     binding]"
                     /db_xref="CDD:438968"
     Site            524..527
                     /site_type="other"
                     /note="PWWP sequence motif"
                     /db_xref="CDD:438968"
     CDS             1..609
                     /gene="LOC144112695"
                     /coded_by="XM_077645521.1:669..2498"
                     /db_xref="GeneID:144112695"
ORIGIN      
        1 magaaeddgk llkgskisvm vedalidvii vsfnysgkvf qgalldvtkk nipfgispwg
       61 algsqdqrgs stqtlgdavv ekgdkfhalr qrhtyfqeks teelplssrs waksrkatgr
      121 nsrdqsvrmr rlrprqvlcs hcraicnens envqlasikk esppvlsslg cvpvskcepd
      181 vparipsktg vandddtmii qtspsprtee sprpkhavya svaesldlkn aqlptqagae
      241 efqskseyqa valmppavnr milrkrkcdd tedlpqsagp melatphskl vpvraaltkt
      301 spvikisfan pqgkgtvvki paklqstgas dtdgdssiec spvrprdtss araakkalkk
      361 arrdhhkgpl lapaspslak hkvkhhkhkl krkrrhretp pdeprndven spsyglegse
      421 aaapaeafle egrshklsis lrrlgakaym rcspkyddll assgsgsecg dvpefpkldp
      481 mrdmdkvkpl mmristhtvk rctldggrem avgdivwgki hgfpwwpgkv lalsvsqrdn
      541 gaavnqqahv awygsstssy mpchqlspfl ddfktrynkk krgpykeair qatleaaacq
      601 elppdlall
//
LOCUS       XP_077501648             362 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112696 [Amblyomma americanum].
ACCESSION   XP_077501648
VERSION     XP_077501648.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645522.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..362
                     /product="uncharacterized protein LOC144112696"
                     /calculated_mol_wt=40858
     Region          90..168
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..362
                     /gene="LOC144112696"
                     /coded_by="XM_077645522.1:11..1099"
                     /db_xref="GeneID:144112696"
ORIGIN      
        1 mnhsadcmva vgsrrrtqps yesaaflfll frpttvwsrg gsclcsmvvi lvrfagtnal
       61 gaesgykmaa meivpkesss kqrsrtrttn wgdvetlqli elwhsfrhdl qltkkrrpif
      121 eqmsvhmksl gfkrdaneiq gritnlayly rregklrlen plrgpwkfwd svhrilrdep
      181 srrapvqarq ilpkpllpna earlippdtt ltvvnvplpe isivrvdhps vpestsagdg
      241 rksplhesta aaeawknksa lsivngdhsd dgqgsdsyph ahsslpsecs rdilktvieq
      301 hrlmalqiln eedirheerv rivldmenrl kqehdnfkla lvdrlnkgfa qilmamksst
      361 ts
//
LOCUS       XP_077501649             218 aa            linear   INV 25-NOV-2025
DEFINITION  transmembrane p24 trafficking protein eclair [Amblyomma
            americanum].
ACCESSION   XP_077501649
VERSION     XP_077501649.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645523.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..218
                     /product="transmembrane p24 trafficking protein eclair"
                     /calculated_mol_wt=25162
     Region          22..213
                     /region_name="EMP24_GP25L"
                     /note="emp24/gp25L/p24 family/GOLD; pfam01105"
                     /db_xref="CDD:426051"
     CDS             1..218
                     /gene="eca"
                     /coded_by="XM_077645523.1:107..763"
                     /db_xref="GeneID:144112697"
ORIGIN      
        1 mfaakhypiv clilsflhys aglyfhiaet erkcfieevp eetlvvgnyk cqlfdpktgg
       61 fmqsspgigm hveirdpedk tilskvysse grfsftshsp gehviclysn ssswfsgsql
      121 rvhldiqvge havdygsvar kekltelqlr vrqlldqvdq itkeqnyqrf reerfrqtse
      181 stnsrvlwws agqtiilllm gfwqmrhlks ffeakklv
//
LOCUS       XP_077501650             535 aa            linear   INV 25-NOV-2025
DEFINITION  MSL complex subunit 3-like [Amblyomma americanum].
ACCESSION   XP_077501650
VERSION     XP_077501650.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645524.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..535
                     /product="MSL complex subunit 3-like"
                     /calculated_mol_wt=59319
     Region          38..103
                     /region_name="CD_CSD"
                     /note="CHROMO (CHRromatin Organization Modifier) domains
                     and chromo shadow domains; cl28914"
                     /db_xref="CDD:475127"
     Site            order(54..57,77,79..82,86,88..91,93..95,98..99)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:350845"
     Site            order(54,79,82,86)
                     /site_type="other"
                     /note="putative methylated histone tail binding site
                     [chemical binding]"
                     /db_xref="CDD:350845"
     Site            order(76,78..83,85..89)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:350845"
     Site            order(76,79,83,85,89)
                     /site_type="other"
                     /note="putative RNA binding site [nucleotide binding]"
                     /db_xref="CDD:350845"
     Region          176..517
                     /region_name="MRG"
                     /note="pfam05712"
                     /db_xref="CDD:461721"
     CDS             1..535
                     /gene="LOC144112699"
                     /coded_by="XM_077645524.1:115..1722"
                     /db_xref="GeneID:144112699"
ORIGIN      
        1 merrcrgqnc krhccrekpq dpaagsasgv kfkfsegetv lcyepdsska kvlydskvlk
       61 lvvgkdsrgr nvpeylihfi gwkrswdccv aeelilpntp enknlkkkle deaaqkllqt
      121 srskkpkvpa ilkeslgrkl hgssrkvqrs gsysrgeesd ssqsessekq sdfsdeedsd
      181 eepqevatdf ninipqilrt kleddfynit skkklvhvpc tpdvvdilea flkqyaeklt
      241 gtaarpakgs slrqppldss eiearfslck eamdglritf sytlsnllly nveqsqfklv
      301 tascrpiriy npapsrqsde ptslnetsll niapdvattv kchsrkslrg nsdlksstss
      361 qrkrsasppe krtlrsgepl siwssspaks tqddsdtdnl alavaimgma prrrtastcs
      421 vtssvsatat tseaavsivn skfdctilsn efleedpssp allygahhfl rlfvklpell
      481 qkmaitkmka sylesfvnel lqymagqhee lfpptaygda eqimaelgkq ktcne
//
LOCUS       XP_077501651             503 aa            linear   INV 25-NOV-2025
DEFINITION  rhotekin-2-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501651
VERSION     XP_077501651.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645525.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..503
                     /product="rhotekin-2-like isoform X1"
                     /calculated_mol_wt=57148
     Region          49..100
                     /region_name="HR1"
                     /note="Protein kinase C-related kinase homology region 1
                     (HR1) domain that binds Rho family small GTPases; cl00087"
                     /db_xref="CDD:469609"
     Region          119..263
                     /region_name="Anillin"
                     /note="Cell division protein anillin; pfam08174"
                     /db_xref="CDD:462393"
     Region          302..410
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     CDS             1..503
                     /gene="LOC144112700"
                     /coded_by="XM_077645525.1:35..1546"
                     /db_xref="GeneID:144112700"
ORIGIN      
        1 maptlkasrr gekrsanven nfdylrflse prkerlrrkh tfsgrkeydl eqkidleikm
       61 regttkllav cqhrpqslea akslltsner maayvaelqr rksadrpkqs igvfpldvsr
      121 srvavseirm pliwkdtdhf knkgdyrrfa vfcllkigte iydtamisnv drsmtdvsfe
      181 dtivfnhipp dfefklevys hilqddlsma stprkiktki tnsvsraigr klaaslkeel
      241 ssleigpkfe leahatlrls daddkvhtyd ltmenlenpq hqlplfghfc cklavvpdct
      301 heeqlsgyls vvqlangvng rrrlwcvlkn fqlclwtspe darhlppvls ipintdtrlr
      361 tdgcsskymh amrictavad kvqehlmvad geqdftkwll hlrqhirdhe lwgnlalkhm
      421 eipspgprra pmflharqms lydetslkds pqglsgsvlq esshhispas vrsrssstss
      481 asssvsgsrn fpllpakktf gyf
//
LOCUS       XP_077501652             502 aa            linear   INV 25-NOV-2025
DEFINITION  rhotekin-2-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077501652
VERSION     XP_077501652.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645526.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..502
                     /product="rhotekin-2-like isoform X2"
                     /calculated_mol_wt=56591
     Region          48..99
                     /region_name="HR1"
                     /note="Protein kinase C-related kinase homology region 1
                     (HR1) domain that binds Rho family small GTPases; cl00087"
                     /db_xref="CDD:469609"
     Region          118..262
                     /region_name="Anillin"
                     /note="Cell division protein anillin; pfam08174"
                     /db_xref="CDD:462393"
     Region          301..409
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     CDS             1..502
                     /gene="LOC144112700"
                     /coded_by="XM_077645526.1:219..1727"
                     /db_xref="GeneID:144112700"
ORIGIN      
        1 mdscfnarsl fgghsstdtl fssaqveviq dldlyfirqi ahslgeydle qkidleikmr
       61 egttkllavc qhrpqsleaa kslltsnerm aayvaelqrr ksadrpkqsi gvfpldvsrs
      121 rvavseirmp liwkdtdhfk nkgdyrrfav fcllkigtei ydtamisnvd rsmtdvsfed
      181 tivfnhippd fefklevysh ilqddlsmas tprkiktkit nsvsraigrk laaslkeels
      241 sleigpkfel eahatlrlsd addkvhtydl tmenlenpqh qlplfghfcc klavvpdcth
      301 eeqlsgylsv vqlangvngr rrlwcvlknf qlclwtsped arhlppvlsi pintdtrlrt
      361 dgcsskymha mrictavadk vqehlmvadg eqdftkwllh lrqhirdhel wgnlalkhme
      421 ipspgprrap mflharqmsl ydetslkdsp qglsgsvlqe sshhispasv rsrssstssa
      481 sssvsgsrnf pllpakktfg yf
//
LOCUS       XP_077501653             478 aa            linear   INV 25-NOV-2025
DEFINITION  rhotekin-2-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077501653
VERSION     XP_077501653.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645527.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..478
                     /product="rhotekin-2-like isoform X3"
                     /calculated_mol_wt=54163
     Region          24..75
                     /region_name="HR1"
                     /note="Protein kinase C-related kinase homology region 1
                     (HR1) domain that binds Rho family small GTPases; cl00087"
                     /db_xref="CDD:469609"
     Region          94..238
                     /region_name="Anillin"
                     /note="Cell division protein anillin; pfam08174"
                     /db_xref="CDD:462393"
     Region          277..385
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     CDS             1..478
                     /gene="LOC144112700"
                     /coded_by="XM_077645527.1:93..1529"
                     /db_xref="GeneID:144112700"
ORIGIN      
        1 mqryiyalvd rgvlfcyipt eeydleqkid leikmregtt kllavcqhrp qsleaaksll
       61 tsnermaayv aelqrrksad rpkqsigvfp ldvsrsrvav seirmpliwk dtdhfknkgd
      121 yrrfavfcll kigteiydta misnvdrsmt dvsfedtivf nhippdfefk levyshilqd
      181 dlsmastprk iktkitnsvs raigrklaas lkeelsslei gpkfeleaha tlrlsdaddk
      241 vhtydltmen lenpqhqlpl fghfccklav vpdctheeql sgylsvvqla ngvngrrrlw
      301 cvlknfqlcl wtspedarhl ppvlsipint dtrlrtdgcs skymhamric tavadkvqeh
      361 lmvadgeqdf tkwllhlrqh irdhelwgnl alkhmeipsp gprrapmflh arqmslydet
      421 slkdspqgls gsvlqesshh ispasvrsrs sstssasssv sgsrnfpllp akktfgyf
//
LOCUS       XP_077501654             444 aa            linear   INV 25-NOV-2025
DEFINITION  rhotekin-2-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077501654
VERSION     XP_077501654.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645528.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..444
                     /product="rhotekin-2-like isoform X4"
                     /calculated_mol_wt=50012
     Region          60..204
                     /region_name="Anillin"
                     /note="Cell division protein anillin; pfam08174"
                     /db_xref="CDD:462393"
     Region          243..351
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     CDS             1..444
                     /gene="LOC144112700"
                     /coded_by="XM_077645528.1:198..1532"
                     /db_xref="GeneID:144112700"
ORIGIN      
        1 mregttklla vcqhrpqsle aakslltsne rmaayvaelq rrksadrpkq sigvfpldvs
       61 rsrvavseir mpliwkdtdh fknkgdyrrf avfcllkigt eiydtamisn vdrsmtdvsf
      121 edtivfnhip pdfefklevy shilqddlsm astprkiktk itnsvsraig rklaaslkee
      181 lssleigpkf eleahatlrl sdaddkvhty dltmenlenp qhqlplfghf ccklavvpdc
      241 theeqlsgyl svvqlangvn grrrlwcvlk nfqlclwtsp edarhlppvl sipintdtrl
      301 rtdgcsskym hamrictava dkvqehlmva dgeqdftkwl lhlrqhirdh elwgnlalkh
      361 meipspgprr apmflharqm slydetslkd spqglsgsvl qesshhispa svrsrsssts
      421 sasssvsgsr nfpllpakkt fgyf
//
LOCUS       XP_077501655             114 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112701 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501655
VERSION     XP_077501655.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645529.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..114
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..114
                     /product="uncharacterized protein LOC144112701 isoform X1"
                     /calculated_mol_wt=12404
     CDS             1..114
                     /gene="LOC144112701"
                     /coded_by="XM_077645529.1:1897..2241"
                     /db_xref="GeneID:144112701"
ORIGIN      
        1 mapmcycvil qmakvnleps tepstaaali aqhrflgaak apkrhlfagd gyhlsagegv
       61 eelaeilvrs lekihgpgil apfsrvptrh iiyachhcgt kghvnenrye ycrp
//
LOCUS       XP_077501656             106 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112701 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501656
VERSION     XP_077501656.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645530.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..106
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..106
                     /product="uncharacterized protein LOC144112701 isoform X2"
                     /calculated_mol_wt=11996
     CDS             1..106
                     /gene="LOC144112701"
                     /coded_by="XM_077645530.1:937..1257"
                     /db_xref="GeneID:144112701"
ORIGIN      
        1 mhgctlqyqw lrmsvcrfil knmkhrflga akapkrhlfa gdgyhlsage gveelaeilv
       61 rslekihgpg ilapfsrvpt rhiiyachhc gtkghvnenr yeycrp
//
LOCUS       XP_077501657             214 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112702 [Amblyomma americanum].
ACCESSION   XP_077501657
VERSION     XP_077501657.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645531.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..214
                     /product="uncharacterized protein LOC144112702"
                     /calculated_mol_wt=23846
     Region          76..96
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(76,79,92,96)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(81,83,85,87..88,91..92,95,109,111,115..116,119..120,
                     123,137,139,141,143..144,147..148,151)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          99..>152
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          104..124
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          <127..>181
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          132..152
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          163..182
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..214
                     /gene="LOC144112702"
                     /coded_by="XM_077645531.1:98..742"
                     /db_xref="GeneID:144112702"
ORIGIN      
        1 meggalplgs tslaprvaae gfwrpfapdp dsrlhcaake alrrpeaads rrrrpvalap
       61 spnsafrpvr vkrlwcsycg vsflnngqlv ghirvhtgdr pykceacera farneeltrh
      121 rlihsgdkph acdvcakrfa rkdhlskhrk thldeahkrv hvcsqpgcgh rytrtdaltr
      181 hqwtahairr dtshcrcrpp datisaaaag hsli
//
LOCUS       XP_077501658            1231 aa            linear   INV 25-NOV-2025
DEFINITION  attractin-like protein dsd [Amblyomma americanum].
ACCESSION   XP_077501658
VERSION     XP_077501658.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645532.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1231
                     /product="attractin-like protein dsd"
                     /calculated_mol_wt=135978
     Region          80..195
                     /region_name="CUB"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; cd00041"
                     /db_xref="CDD:238001"
     Site            order(90,92,94,120,125,166,190,192,194..195)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     Region          198..225
                     /region_name="EGF_Tenascin"
                     /note="Tenascin EGF domain; cl46594"
                     /db_xref="CDD:480934"
     Region          <276..360
                     /region_name="NanM"
                     /note="N-acetylneuraminic acid mutarotase [Cell
                     wall/membrane/envelope biogenesis]; COG3055"
                     /db_xref="CDD:442289"
     Region          277..320
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          320..568
                     /region_name="NanM"
                     /note="N-acetylneuraminic acid mutarotase [Cell
                     wall/membrane/envelope biogenesis]; COG3055"
                     /db_xref="CDD:442289"
     Region          328..385
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          387..427
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          435..485
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          490..548
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          745..789
                     /region_name="PSI"
                     /note="Plexin repeat; pfam01437"
                     /db_xref="CDD:396154"
     Region          862..907
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(862,864,870,876,880,889)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          908..955
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(909,911,918,925,927,937)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     CDS             1..1231
                     /gene="dsd"
                     /coded_by="XM_077645532.1:53..3748"
                     /db_xref="GeneID:144112703"
ORIGIN      
        1 mmefvicfcm vrrksrrkrl hlfdprcwlm ilfvlllcaw qlsdaqrtfc gdtecwhgnc
       61 tgdgrclcyp gwqgpgcqrc ggrvrltepn gvitngigny skdmkctwli esgirnatir
      121 fefthfetec gwdhlyvydg dsmfapmvaa ysglllskgs stklpeivth sgsafvhfys
      181 davynmsgfn ltysvngcsr ncsshgecvd gacqcspgws gddcstqvcp qdcnggacdk
      241 eeghcicdts mkgedcsiav saphyeaqpa tgwvrgramh qalilgdamw ilggesftel
      301 dqqiakydfi arkwhtvkpt ttsapanryg hsvvmyrgkl ylfggtlrdg rtsndlwrwd
      361 peelrwaqls phqpecqnel cppltasght aaivdntmvv ifghnpdygy lnvvqeytfg
      421 tnkweivqak gaivkggygh ssvydpvrrq ilvfggyhsq tttgvvvdyl ysyqplertw
      481 tllapsashr ylhsavllhr mmlvfggnth ndtafstgdk cfsadllayd iecdswqtls
      541 vpsssylqle rfghsavvyn esmyifggfn gqmltsivkf tpgscrsfst pekcitsatg
      601 ikcawnpdkk acqafhlfna tkgihtcprk saanltvlce kqtscpsclq nnygcvwcgt
      661 vcahkrcpkg skgladaren qtdveqcqqs essncdklhn chachtefhc gwqndhrcyt
      721 fvrdsgnkte kavlsdnyrv kcdnscssrt tckacilgsc mwcisqqrcl etnayaatfp
      781 laqctewitt kcsalscedf qtcsacqehe rcgwcddgsn tgkgqclegs asgplspags
      841 tyrvngslcp apnwyftdcp kcqcnghstc ngsseclqsc qhrtegphce hciagyhgnp
      901 anggnctpcf cnnhgtfchr etgkcncttk giigpncdrc deqnhyvgsp qeeggtcfyn
      961 lsidfqytfn lssedyyrni nfmnvpikvg ndveftiscs gkayvnisig sggqppsrad
     1021 gdaepallds rkcdniklpf ydekhnfgsq nttffvyvfn fttpfvlqis fsqhraldll
     1081 qffitfsscf lslliiaail wkikqkydly rrrqqlfvem eqmasrpfag vtleldsepd
     1141 gsskqfqdgm kqaqghptpv alepcasgka avltlivrmp tggqehtatg qsglaiasal
     1201 vslgtlcksd tikedgksdc wkpsshpgtc i
//
LOCUS       XP_077501659             275 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112706 [Amblyomma americanum].
ACCESSION   XP_077501659
VERSION     XP_077501659.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645533.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..275
                     /product="uncharacterized protein LOC144112706"
                     /calculated_mol_wt=30333
     Region          96..170
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..275
                     /gene="LOC144112706"
                     /coded_by="XM_077645533.1:156..983"
                     /db_xref="GeneID:144112706"
ORIGIN      
        1 magvfrsslq ttveiavgdi tktvtavrss dgscipdgng ylyegadger yvlvfedeht
       61 pasqprasde sgigasasae avapipasae adlelwsgak trflidkyvq fkdlvgqkgg
      121 frtkkmfwtr ltnllnhefg gtlsavqven kwkslersyk rartknntsg hhrvpceyee
      181 elskvlekeh hiqppallet gasagnsdva aedmnssrdm eevpasmprr krqrtssnsv
      241 laetllklda arakrheerm akfdmlievm rtnkq
//
LOCUS       XP_077501660             830 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112707 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501660
VERSION     XP_077501660.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645534.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..830
                     /product="uncharacterized protein LOC144112707 isoform X1"
                     /calculated_mol_wt=89418
     Region          172..263
                     /region_name="FHA"
                     /note="forkhead associated (FHA) domain superfamily;
                     cl00062"
                     /db_xref="CDD:469597"
     Region          <381..>828
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..830
                     /gene="LOC144112707"
                     /coded_by="XM_077645534.1:587..3079"
                     /db_xref="GeneID:144112707"
ORIGIN      
        1 mdcsqspset sasvrragep aavsgepvsk kyvqsrssqs lllfqkdadg asptgstlnq
       61 acshasgfim vqsdhtyagp steasassle pgtagvaatl sasadlspga pfsssplsce
      121 gsftspiner rplqklrakl rqlrnrnktl qglllqrrrm kttaelmeyy pkivtlrrng
      181 tgfsvlplit grcsvggaag cdiriprsgs hcclflvgkn eqariinctg tadlklngkd
      241 vhdysvlshg diveisyrkf rfdygekrkk ensskvaaas hqpkaagdat ptsnnkskka
      301 steqagavac eegddeeegv plqsardisq scqeslehms vvaespkvtv skqtfqyssq
      361 ileisresap ekatlptrsg rssqtssneg klkvqekeke atvssnqkps skpskakrtp
      421 askrataadv ttelekagms asknlafdnt dvtaaafgra traarpkyve tdkrtdvkmp
      481 rqeakrtvsp lktleaeqrp asnkelgetp kqeaadtkks lrkilqakta rstrkvnege
      541 evpelgtagv spskcssatn vhvldeparl trgcrtrgsr kdiveapkek kvdqpqstka
      601 traehtqgsr kvckegetlq dvtgsslvma sttqrkdvtf dkhlqreasp tkevsvtrnt
      661 rakrkvdlke esiaekhtta edligtvaek sgghqqrvas geyapqendv qcsnsakqvi
      721 npkitrdkev vddapaspnv evatvepasa ggkalattme pkrskrtmaa vkvdvaqtan
      781 eatprktwgr raealktale waddkpgasr rtakdrseee pppkrtrkmv
//
LOCUS       XP_077501661             664 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112707 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501661
VERSION     XP_077501661.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645535.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..664
                     /product="uncharacterized protein LOC144112707 isoform X2"
                     /calculated_mol_wt=71979
     Region          6..97
                     /region_name="FHA"
                     /note="forkhead associated (FHA) domain superfamily;
                     cl00062"
                     /db_xref="CDD:469597"
     Region          <215..>662
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..664
                     /gene="LOC144112707"
                     /coded_by="XM_077645535.1:7079..9073"
                     /db_xref="GeneID:144112707"
ORIGIN      
        1 meyypkivtl rrngtgfsvl plitgrcsvg gaagcdirip rsgshcclfl vgkneqarii
       61 nctgtadlkl ngkdvhdysv lshgdiveis yrkfrfdyge krkkensskv aaashqpkaa
      121 gdatptsnnk skkasteqag avaceegdde eegvplqsar disqscqesl ehmsvvaesp
      181 kvtvskqtfq yssqileisr esapekatlp trsgrssqts snegklkvqe kekeatvssn
      241 qkpsskpska krtpaskrat aadvttelek agmsasknla fdntdvtaaa fgratraarp
      301 kyvetdkrtd vkmprqeakr tvsplktlea eqrpasnkel getpkqeaad tkkslrkilq
      361 aktarstrkv negeevpelg tagvspskcs satnvhvlde parltrgcrt rgsrkdivea
      421 pkekkvdqpq stkatraeht qgsrkvckeg etlqdvtgss lvmasttqrk dvtfdkhlqr
      481 easptkevsv trntrakrkv dlkeesiaek httaedligt vaeksgghqq rvasgeyapq
      541 endvqcsnsa kqvinpkitr dkevvddapa spnvevatve pasaggkala ttmepkrskr
      601 tmaavkvdva qtaneatprk twgrraealk talewaddkp gasrrtakdr seeepppkrt
      661 rkmv
//
LOCUS       XP_077501662             664 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112707 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501662
VERSION     XP_077501662.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645536.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..664
                     /product="uncharacterized protein LOC144112707 isoform X2"
                     /calculated_mol_wt=71979
     Region          6..97
                     /region_name="FHA"
                     /note="forkhead associated (FHA) domain superfamily;
                     cl00062"
                     /db_xref="CDD:469597"
     Region          <215..>662
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..664
                     /gene="LOC144112707"
                     /coded_by="XM_077645536.1:4223..6217"
                     /db_xref="GeneID:144112707"
ORIGIN      
        1 meyypkivtl rrngtgfsvl plitgrcsvg gaagcdirip rsgshcclfl vgkneqarii
       61 nctgtadlkl ngkdvhdysv lshgdiveis yrkfrfdyge krkkensskv aaashqpkaa
      121 gdatptsnnk skkasteqag avaceegdde eegvplqsar disqscqesl ehmsvvaesp
      181 kvtvskqtfq yssqileisr esapekatlp trsgrssqts snegklkvqe kekeatvssn
      241 qkpsskpska krtpaskrat aadvttelek agmsasknla fdntdvtaaa fgratraarp
      301 kyvetdkrtd vkmprqeakr tvsplktlea eqrpasnkel getpkqeaad tkkslrkilq
      361 aktarstrkv negeevpelg tagvspskcs satnvhvlde parltrgcrt rgsrkdivea
      421 pkekkvdqpq stkatraeht qgsrkvckeg etlqdvtgss lvmasttqrk dvtfdkhlqr
      481 easptkevsv trntrakrkv dlkeesiaek httaedligt vaeksgghqq rvasgeyapq
      541 endvqcsnsa kqvinpkitr dkevvddapa spnvevatve pasaggkala ttmepkrskr
      601 tmaavkvdva qtaneatprk twgrraealk talewaddkp gasrrtakdr seeepppkrt
      661 rkmv
//
LOCUS       XP_077501663             157 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112708 [Amblyomma americanum].
ACCESSION   XP_077501663
VERSION     XP_077501663.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645537.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..157
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..157
                     /product="uncharacterized protein LOC144112708"
                     /calculated_mol_wt=17382
     CDS             1..157
                     /gene="LOC144112708"
                     /coded_by="XM_077645537.1:181..654"
                     /db_xref="GeneID:144112708"
ORIGIN      
        1 mpstkwysqp tqrrighdnv llaaailftr cspteplrlv enagicsfsk ctydriqkdi
       61 lfpapytgph lvvvrgekmp cvisydtvtp lnyikfahid ntmlpssaal sllfplflnk
      121 tslcplspsr ktagttlspe gealwwhvsa mrcevkn
//
LOCUS       XP_077501664             222 aa            linear   INV 25-NOV-2025
DEFINITION  HEAT repeat-containing protein 1-like [Amblyomma americanum].
ACCESSION   XP_077501664
VERSION     XP_077501664.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645538.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..222
                     /product="HEAT repeat-containing protein 1-like"
                     /calculated_mol_wt=24166
     CDS             1..222
                     /gene="LOC144112710"
                     /coded_by="XM_077645538.1:204..872"
                     /db_xref="GeneID:144112710"
ORIGIN      
        1 mrcsreawsw acppmpstyl hqcvltalqa hgllaprqel aslvkclris qtaqtqqlvl
       61 gllslaalhy peevlhnvts vftykgasll wqvdncsfqt vmptvktvmp allqactges
      121 ksaeaaesav asvtrvfvgp fldipehwwl slfaklattl gagdhlwila aqmaehqvtk
      181 mggtdvdevt fvrymsgrmk frltlrcscc rtlsgadvpp ag
//
LOCUS       XP_077501665             196 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112711 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501665
VERSION     XP_077501665.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645539.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..196
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..196
                     /product="uncharacterized protein LOC144112711 isoform X1"
                     /calculated_mol_wt=21728
     CDS             1..196
                     /gene="LOC144112711"
                     /coded_by="XM_077645539.1:430..1020"
                     /db_xref="GeneID:144112711"
ORIGIN      
        1 mpavvpspaa vargrhspfq rrsfslwlsl frpsccrqpr tssgswpnri lchravttva
       61 strdpravcq paqvalnssh kgcsrphllf keidqhaaev aadteellel ivtwshglkk
      121 qntllvllnh pqvpvamqng ilqlvthvds kvcgllmmtt ggfdfggrkp lrqrctvavm
      181 flvctmwesk trfffp
//
LOCUS       XP_077501666             191 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112711 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501666
VERSION     XP_077501666.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645540.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..191
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..191
                     /product="uncharacterized protein LOC144112711 isoform X2"
                     /calculated_mol_wt=21049
     CDS             1..191
                     /gene="LOC144112711"
                     /coded_by="XM_077645540.1:430..1005"
                     /db_xref="GeneID:144112711"
ORIGIN      
        1 mpavvpspaa vargrhspfq rrsfslwlsl frpsccrqpr tssgswpnri lchravttva
       61 strdpravcq paqvalnssh kgcsrphllf keidqhaaev aadteellel ivtwshglkk
      121 qntllvllnh pqvpvamqng ilqlvthvds kvtsclqlga lytsearecn dsptsfasfh
      181 gedfdhhwqp c
//
LOCUS       XP_077501667             114 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112712 [Amblyomma americanum].
ACCESSION   XP_077501667
VERSION     XP_077501667.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645541.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..114
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..114
                     /product="uncharacterized protein LOC144112712"
                     /calculated_mol_wt=12695
     CDS             1..114
                     /gene="LOC144112712"
                     /coded_by="XM_077645541.1:1..345"
                     /db_xref="GeneID:144112712"
ORIGIN      
        1 mttmgrqhrr llangaalpr vnarrsvdlh lvhglftsla rqtqtdvdek fardslqirl
       61 agtepelvqa vlalgkdlck fleqgvvlgt lrrllfepte qskkwfdlrt gvcv
//
LOCUS       XP_077501668             206 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112715 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501668
VERSION     XP_077501668.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645542.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..206
                     /product="uncharacterized protein LOC144112715 isoform X1"
                     /calculated_mol_wt=22286
     CDS             1..206
                     /gene="LOC144112715"
                     /coded_by="XM_077645542.1:988..1608"
                     /db_xref="GeneID:144112715"
ORIGIN      
        1 meacctlcnv hicpdsacrp rlsgrlpwps kpsevgpavi dplqclapra smvvvctafl
       61 schpqpstta pvarssplst erpplhipge glvarfswgr haqaqtqich gdcccycpat
      121 vceltaagre rgatrgqvpl llhekgggdv veelyppraf pamvregpai hdrpeqrwwk
      181 eqlsnahnpf ccharlstsk qhvsnv
//
LOCUS       XP_077501669             204 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112715 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501669
VERSION     XP_077501669.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645543.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..204
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..204
                     /product="uncharacterized protein LOC144112715 isoform X2"
                     /calculated_mol_wt=22093
     CDS             1..204
                     /gene="LOC144112715"
                     /coded_by="XM_077645543.1:992..1606"
                     /db_xref="GeneID:144112715"
ORIGIN      
        1 meacctlcnv hicpdsacrp rlsgrlpwps kpsevgpavi dplqclapra smvvvctafl
       61 schpqpstta pvarssplst erpplhipge glvarfswgr haqaqtqich gdcccycpat
      121 vceltaagre rgatrgqvpl llhekgggdv veelyppraf pamvregpai hdrpeqrwwk
      181 eqlsqpnagr qpkvgdpphr rsfl
//
LOCUS       XP_077501670             199 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112715 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501670
VERSION     XP_077501670.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645544.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..199
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..199
                     /product="uncharacterized protein LOC144112715 isoform X3"
                     /calculated_mol_wt=21611
     CDS             1..199
                     /gene="LOC144112715"
                     /coded_by="XM_077645544.1:981..1580"
                     /db_xref="GeneID:144112715"
ORIGIN      
        1 meacctlcnv hicpdsacrp rlsgrlpwps kpsevgpavi dplqclapra smvvvctafl
       61 schpqpstta pvarssplst erpplhipge glvarfswgr haqaqtqich gdcccycpat
      121 vceltaagre rgatrgqvpl llhekgggdv veelyppraf pamvregpai hdrpeqrwwk
      181 eqlsvpmcnp wflrllgmv
//
LOCUS       XP_077501671             186 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112715 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077501671
VERSION     XP_077501671.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645545.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..186
                     /product="uncharacterized protein LOC144112715 isoform X4"
                     /calculated_mol_wt=20009
     CDS             1..186
                     /gene="LOC144112715"
                     /coded_by="XM_077645545.1:986..1546"
                     /db_xref="GeneID:144112715"
ORIGIN      
        1 meacctlcnv hicpdsacrp rlsgrlpwps kpsevgpavi dplqclapra smvvvctafl
       61 schpqpstta pvarssplst erpplhipge glvarfswgr haqaqtqich gdcccycpat
      121 vceltaagre rgatrgqvpl llhekgggdv veelyppraf pamvregpai hdrpeqrwwk
      181 eqlsvg
//
LOCUS       XP_077501672             186 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112715 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077501672
VERSION     XP_077501672.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645546.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..186
                     /product="uncharacterized protein LOC144112715 isoform X4"
                     /calculated_mol_wt=20009
     CDS             1..186
                     /gene="LOC144112715"
                     /coded_by="XM_077645546.1:986..1546"
                     /db_xref="GeneID:144112715"
ORIGIN      
        1 meacctlcnv hicpdsacrp rlsgrlpwps kpsevgpavi dplqclapra smvvvctafl
       61 schpqpstta pvarssplst erpplhipge glvarfswgr haqaqtqich gdcccycpat
      121 vceltaagre rgatrgqvpl llhekgggdv veelyppraf pamvregpai hdrpeqrwwk
      181 eqlsvg
//
LOCUS       XP_077501673             161 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112715 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077501673
VERSION     XP_077501673.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645547.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..161
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..161
                     /product="uncharacterized protein LOC144112715 isoform X5"
                     /calculated_mol_wt=18348
     CDS             1..161
                     /gene="LOC144112715"
                     /coded_by="XM_077645547.1:595..1080"
                     /db_xref="GeneID:144112715"
ORIGIN      
        1 mqwmdgwtdg rtdgrtsqki rrptithqsw ralpgdffvl apssvpricv dkdrypfcft
       61 rkeeetswrs sirhalfqrw fvkvqpfttd qnsgggrnnf rnpmqadnpr sailhtavvs
      121 fetawnavrs hqttmvhlsa taeqgfvdld flpgaahawp l
//
LOCUS       XP_077501674             100 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112717 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501674
VERSION     XP_077501674.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645548.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..100
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..100
                     /product="uncharacterized protein LOC144112717 isoform X1"
                     /calculated_mol_wt=11132
     CDS             1..100
                     /gene="LOC144112717"
                     /coded_by="XM_077645548.1:165..467"
                     /db_xref="GeneID:144112717"
ORIGIN      
        1 mefsktalty rtqhhagaag prrpvgetay hdcrriegly syptgiitll lhllssiiep
       61 tlpqpevayq plipdikhvt ckrialptas dklilnswlw
//
LOCUS       XP_077501675              91 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112717 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501675
VERSION     XP_077501675.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645549.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..91
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..91
                     /product="uncharacterized protein LOC144112717 isoform X2"
                     /calculated_mol_wt=10628
     CDS             1..91
                     /gene="LOC144112717"
                     /coded_by="XM_077645549.1:525..800"
                     /db_xref="GeneID:144112717"
ORIGIN      
        1 mqhaalpcfi dqgrypfcft rkeeetswrs sirhalfqrw fvkvqpfttd qnsgggrnnf
       61 rwvkysnrak tntdkrggtt krrhesavfl s
//
LOCUS       XP_077501676              84 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112717 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501676
VERSION     XP_077501676.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645550.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..84
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..84
                     /product="uncharacterized protein LOC144112717 isoform X3"
                     /calculated_mol_wt=9694
     CDS             1..84
                     /gene="LOC144112717"
                     /coded_by="XM_077645550.1:525..779"
                     /db_xref="GeneID:144112717"
ORIGIN      
        1 mqhaalpcfi dqgrypfcft rkeeetswrs sirhalfqrw fvkvqpfttd qnsgggrnnf
       61 raktntdkrg gttkrrhesa vfls
//
LOCUS       XP_077501677             222 aa            linear   INV 25-NOV-2025
DEFINITION  HEAT repeat-containing protein 1-like [Amblyomma americanum].
ACCESSION   XP_077501677
VERSION     XP_077501677.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645551.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..222
                     /product="HEAT repeat-containing protein 1-like"
                     /calculated_mol_wt=24150
     CDS             1..222
                     /gene="LOC144112719"
                     /coded_by="XM_077645551.1:204..872"
                     /db_xref="GeneID:144112719"
ORIGIN      
        1 mrcsreawsw acppmpstyl hqcvltalqa hgllaprqel aslvkclris qtaqtqqlvl
       61 gllslaalhy peevlhnvts vftykgasll wqvdncsfqt vmptvktvmp allqactges
      121 ksaeaaesav asvtrvfvgp fpdipehwwl slfaklattl gagdhlwila aqmaehqvtk
      181 mggtdvdevt fvrymsgrmk frltlrcscc rtlsgadvpp ag
//
LOCUS       XP_077501679             196 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112720 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501679
VERSION     XP_077501679.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645553.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..196
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..196
                     /product="uncharacterized protein LOC144112720 isoform X1"
                     /calculated_mol_wt=21728
     CDS             1..196
                     /gene="LOC144112720"
                     /coded_by="XM_077645553.1:552..1142"
                     /db_xref="GeneID:144112720"
ORIGIN      
        1 mpavvpspaa vargrhspfq rrsfslwlsl frpsccrqpr tssgswpnri lchravttva
       61 strdpravcq paqvalnssh kgcsrphllf keidqhaaev aadteellel ivtwshglkk
      121 qntllvllnh pqvpvamqng ilqlvthvds kvcgllmmtt ggfdfggrkp lrqrctvavm
      181 flvctmwesk trfffp
//
LOCUS       XP_077501680             191 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112720 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501680
VERSION     XP_077501680.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645554.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..191
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..191
                     /product="uncharacterized protein LOC144112720 isoform X2"
                     /calculated_mol_wt=21049
     CDS             1..191
                     /gene="LOC144112720"
                     /coded_by="XM_077645554.1:552..1127"
                     /db_xref="GeneID:144112720"
ORIGIN      
        1 mpavvpspaa vargrhspfq rrsfslwlsl frpsccrqpr tssgswpnri lchravttva
       61 strdpravcq paqvalnssh kgcsrphllf keidqhaaev aadteellel ivtwshglkk
      121 qntllvllnh pqvpvamqng ilqlvthvds kvtsclqlga lytsearecn dsptsfasfh
      181 gedfdhhwqp c
//
LOCUS       XP_077501681             191 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112720 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501681
VERSION     XP_077501681.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645555.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..191
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..191
                     /product="uncharacterized protein LOC144112720 isoform X2"
                     /calculated_mol_wt=21049
     CDS             1..191
                     /gene="LOC144112720"
                     /coded_by="XM_077645555.1:552..1127"
                     /db_xref="GeneID:144112720"
ORIGIN      
        1 mpavvpspaa vargrhspfq rrsfslwlsl frpsccrqpr tssgswpnri lchravttva
       61 strdpravcq paqvalnssh kgcsrphllf keidqhaaev aadteellel ivtwshglkk
      121 qntllvllnh pqvpvamqng ilqlvthvds kvtsclqlga lytsearecn dsptsfasfh
      181 gedfdhhwqp c
//
LOCUS       XP_077501682             178 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112730 [Amblyomma americanum].
ACCESSION   XP_077501682
VERSION     XP_077501682.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645556.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..178
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..178
                     /product="uncharacterized protein LOC144112730"
                     /calculated_mol_wt=19410
     CDS             1..178
                     /gene="LOC144112730"
                     /coded_by="XM_077645556.1:1020..1556"
                     /db_xref="GeneID:144112730"
ORIGIN      
        1 mqklfkvhfl gglwllhlda sccavassgr qgaslslake klqslavavs aqllqtvedi
       61 kwilveqnlv qgirelavsl aqaalnsshk gcsrphllfk eidqhaaeva adteelleli
      121 vtwshglkkq ntllvllnhp qvpvamqngi lqlvthvdsk taqaataarr snfrrdew
//
LOCUS       XP_077501683             236 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112734 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501683
VERSION     XP_077501683.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645557.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..236
                     /product="uncharacterized protein LOC144112734 isoform X1"
                     /calculated_mol_wt=26200
     CDS             1..236
                     /gene="LOC144112734"
                     /coded_by="XM_077645557.1:213..923"
                     /db_xref="GeneID:144112734"
ORIGIN      
        1 mefsktalty rtqhhagaag prrpvgetay hdcrriegly syptgiitll lhllssiiep
       61 tlpqpevayq plipdikhvt ckriatpprr lslfpllarr dllstsreka wsrgspgarw
      121 ssggrrghar tqichgdrcr yrpatvcelt vvseeprgrs splsterppl hipgeglvar
      181 fswgrhaqaq tqichgdrcr ycpatvcelt aagrergatr alptasdkli lnswlw
//
LOCUS       XP_077501684             227 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112734 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501684
VERSION     XP_077501684.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645558.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..227
                     /product="uncharacterized protein LOC144112734 isoform X2"
                     /calculated_mol_wt=25441
     CDS             1..227
                     /gene="LOC144112734"
                     /coded_by="XM_077645558.1:213..896"
                     /db_xref="GeneID:144112734"
ORIGIN      
        1 mefsktalty rtqhhagaag prrpvgetay hdcrriegly syptgiitll lhllssiiep
       61 tlpqpevayq plipdikhvt ckriatpprr lslfpllarr dllstsreka wsrgspgarw
      121 ssggrrghar tqichgdrcr yrpatvcelt vvseeprgvp lllhekgggd vveelyppra
      181 fpamvregpa ihdrpeqrww keqlsnahnp fccharlsts kqhvsnv
//
LOCUS       XP_077501685             221 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112734 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501685
VERSION     XP_077501685.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645559.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..221
                     /product="uncharacterized protein LOC144112734 isoform X3"
                     /calculated_mol_wt=24760
     CDS             1..221
                     /gene="LOC144112734"
                     /coded_by="XM_077645559.1:213..878"
                     /db_xref="GeneID:144112734"
ORIGIN      
        1 mefsktalty rtqhhagaag prrpvgetay hdcrriegly syptgiitll lhllssiiep
       61 tlpqpevayq plipdikhvt ckriatpprr lslfpllarr dllstsreka wsrgspgarw
      121 ssggrrghar tqichgdrcr yrpatvcelt vvseeprgvp lllhekgggd vveelyppra
      181 fpamvregpa ihdrpeqrww keqlsgkdeh gqegrhhkap s
//
LOCUS       XP_077501686             220 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112734 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077501686
VERSION     XP_077501686.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645560.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..220
                     /product="uncharacterized protein LOC144112734 isoform X4"
                     /calculated_mol_wt=24767
     CDS             1..220
                     /gene="LOC144112734"
                     /coded_by="XM_077645560.1:213..875"
                     /db_xref="GeneID:144112734"
ORIGIN      
        1 mefsktalty rtqhhagaag prrpvgetay hdcrriegly syptgiitll lhllssiiep
       61 tlpqpevayq plipdikhvt ckriatpprr lslfpllarr dllstsreka wsrgspgarw
      121 ssggrrghar tqichgdrcr yrpatvcelt vvseeprgvp lllhekgggd vveelyppra
      181 fpamvregpa ihdrpeqrww keqlsvpmcn pwflrllgmv
//
LOCUS       XP_077501687             207 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112734 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077501687
VERSION     XP_077501687.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645561.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..207
                     /product="uncharacterized protein LOC144112734 isoform X5"
                     /calculated_mol_wt=23165
     CDS             1..207
                     /gene="LOC144112734"
                     /coded_by="XM_077645561.1:213..836"
                     /db_xref="GeneID:144112734"
ORIGIN      
        1 mefsktalty rtqhhagaag prrpvgetay hdcrriegly syptgiitll lhllssiiep
       61 tlpqpevayq plipdikhvt ckriatpprr lslfpllarr dllstsreka wsrgspgarw
      121 ssggrrghar tqichgdrcr yrpatvcelt vvseeprgvp lllhekgggd vveelyppra
      181 fpamvregpa ihdrpeqrww keqlsvg
//
LOCUS       XP_077501689             207 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112734 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077501689
VERSION     XP_077501689.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645563.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..207
                     /product="uncharacterized protein LOC144112734 isoform X5"
                     /calculated_mol_wt=23165
     CDS             1..207
                     /gene="LOC144112734"
                     /coded_by="XM_077645563.1:213..836"
                     /db_xref="GeneID:144112734"
ORIGIN      
        1 mefsktalty rtqhhagaag prrpvgetay hdcrriegly syptgiitll lhllssiiep
       61 tlpqpevayq plipdikhvt ckriatpprr lslfpllarr dllstsreka wsrgspgarw
      121 ssggrrghar tqichgdrcr yrpatvcelt vvseeprgvp lllhekgggd vveelyppra
      181 fpamvregpa ihdrpeqrww keqlsvg
//
LOCUS       XP_077501690             207 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112734 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077501690
VERSION     XP_077501690.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645564.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..207
                     /product="uncharacterized protein LOC144112734 isoform X5"
                     /calculated_mol_wt=23165
     CDS             1..207
                     /gene="LOC144112734"
                     /coded_by="XM_077645564.1:213..836"
                     /db_xref="GeneID:144112734"
ORIGIN      
        1 mefsktalty rtqhhagaag prrpvgetay hdcrriegly syptgiitll lhllssiiep
       61 tlpqpevayq plipdikhvt ckriatpprr lslfpllarr dllstsreka wsrgspgarw
      121 ssggrrghar tqichgdrcr yrpatvcelt vvseeprgvp lllhekgggd vveelyppra
      181 fpamvregpa ihdrpeqrww keqlsvg
//
LOCUS       XP_077501691             287 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112736 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501691
VERSION     XP_077501691.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645565.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..287
                     /product="uncharacterized protein LOC144112736 isoform X1"
                     /calculated_mol_wt=31083
     CDS             1..287
                     /gene="LOC144112736"
                     /coded_by="XM_077645565.1:1112..1975"
                     /db_xref="GeneID:144112736"
ORIGIN      
        1 mpavvpspaa vargrhspfq rrsfslwlsl frpsccrqpr tssgswpnri lchravttva
       61 strdpravcq paqvalnssh kgcsrphllf keidqhaaev aadteellel ivtwshglkk
      121 qntllvllnh pqvpvamqng ilqlvthvds kvskdlvkkl peenqamlld tlvdlaldtp
      181 psvqhsavsg alrkvcffgs llveplqrtg vslqcpartv reakkmrtgp srvspvearp
      241 hpagedpepk vdrrghaagw atlcaaqekp gaghvppcrv htctsac
//
LOCUS       XP_077501692             189 aa            linear   INV 25-NOV-2025
DEFINITION  HEAT repeat-containing protein 1-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501692
VERSION     XP_077501692.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645566.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..189
                     /product="HEAT repeat-containing protein 1-like isoform
                     X2"
                     /calculated_mol_wt=20501
     CDS             1..189
                     /gene="LOC144112736"
                     /coded_by="XM_077645566.1:481..1050"
                     /db_xref="GeneID:144112736"
ORIGIN      
        1 mrcsreawsw acppmpstyl hqcvltalqa hgllaprqel aslvkclris qtaqtqqlvl
       61 gllslaalhy peevlhnvts vftykgasll wqvdncsfqt vmptvktvmp allqactges
      121 ksaeaaesav asvtrvfvgp fpdipehwwl slfaklattl gagdhlwila aqmaehqvtk
      181 mggtdvdei
//
LOCUS       XP_077501693             189 aa            linear   INV 25-NOV-2025
DEFINITION  HEAT repeat-containing protein 1-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501693
VERSION     XP_077501693.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645567.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..189
                     /product="HEAT repeat-containing protein 1-like isoform
                     X2"
                     /calculated_mol_wt=20501
     CDS             1..189
                     /gene="LOC144112736"
                     /coded_by="XM_077645567.1:1216..1785"
                     /db_xref="GeneID:144112736"
ORIGIN      
        1 mrcsreawsw acppmpstyl hqcvltalqa hgllaprqel aslvkclris qtaqtqqlvl
       61 gllslaalhy peevlhnvts vftykgasll wqvdncsfqt vmptvktvmp allqactges
      121 ksaeaaesav asvtrvfvgp fpdipehwwl slfaklattl gagdhlwila aqmaehqvtk
      181 mggtdvdei
//
LOCUS       XP_077501694             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112739 [Amblyomma americanum].
ACCESSION   XP_077501694
VERSION     XP_077501694.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645568.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144112739"
                     /calculated_mol_wt=14971
     CDS             1..127
                     /gene="LOC144112739"
                     /coded_by="XM_077645568.1:14..397"
                     /db_xref="GeneID:144112739"
ORIGIN      
        1 msgnlaylrr apegcpstel iriyinrede effeilrrye diikkimmdw sgvediqcdl
       61 ktdrmdewfl qlmvtgsrwa lerlkeivvf prgafarvra geerhrwntr vialmlctgn
      121 riflegv
//
LOCUS       XP_077501696             161 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112748 [Amblyomma americanum].
ACCESSION   XP_077501696
VERSION     XP_077501696.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645570.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..161
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..161
                     /product="uncharacterized protein LOC144112748"
                     /calculated_mol_wt=17572
     CDS             1..161
                     /gene="LOC144112748"
                     /coded_by="XM_077645570.1:116..601"
                     /db_xref="GeneID:144112748"
ORIGIN      
        1 mpsallwtfl ptylvqlwst rsaraaanqf rqlmqklfkm hspslkhqvh flgglwllhl
       61 dasccavass shqgaspsqr ksfslwlslf rpsccrqlrt ssgswsnril chravttvas
      121 trdprggcqp gtggielvsq gvqqapssfq gdrpaccrsg r
//
LOCUS       XP_077501697              85 aa            linear   INV 25-NOV-2025
DEFINITION  inactive selenide, water dikinase-like protein [Amblyomma
            americanum].
ACCESSION   XP_077501697
VERSION     XP_077501697.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645571.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..85
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..85
                     /product="inactive selenide, water dikinase-like protein"
                     /calculated_mol_wt=9001
     Region          1..>80
                     /region_name="PurM-like"
                     /note="AIR (aminoimidazole ribonucleotide) synthase
                     related protein. This family includes Hydrogen
                     expression/formation protein HypE, AIR synthases, FGAM
                     (formylglycinamidine ribonucleotide) synthase and
                     Selenophosphate synthetase (SelD). The N-terminal
                     domain...; cl10019"
                     /db_xref="CDD:471963"
     CDS             1..85
                     /gene="LOC144112749"
                     /coded_by="XM_077645571.1:1..258"
                     /db_xref="GeneID:144112749"
ORIGIN      
        1 mdssgvplgd sgyslvqtid yfypsvedpy iagkiacanv lsdlyalgmt rcdnvlmvma
       61 vstkltekqr divvpllirg sqglg
//
LOCUS       XP_077501698             179 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112753 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501698
VERSION     XP_077501698.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645572.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..179
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..179
                     /product="uncharacterized protein LOC144112753 isoform X1"
                     /calculated_mol_wt=19429
     CDS             1..179
                     /gene="LOC144112753"
                     /coded_by="XM_077645572.1:100..639"
                     /db_xref="GeneID:144112753"
ORIGIN      
        1 mcsaivlsll spvqgirela vslaqaalns shkgcsrphl lfkeidqhaa evaadteell
       61 elivtwshgl kkqntllvll nhpqvpvamq ngilqlvthv dskvtsclql galytleare
      121 cndsptsfvs fhgedfdhhw qpcwtasswi kasgstmsst lvvdkncwrv pelfsgaat
//
LOCUS       XP_077501700             179 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112753 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501700
VERSION     XP_077501700.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645574.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..179
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..179
                     /product="uncharacterized protein LOC144112753 isoform X1"
                     /calculated_mol_wt=19429
     CDS             1..179
                     /gene="LOC144112753"
                     /coded_by="XM_077645574.1:100..639"
                     /db_xref="GeneID:144112753"
ORIGIN      
        1 mcsaivlsll spvqgirela vslaqaalns shkgcsrphl lfkeidqhaa evaadteell
       61 elivtwshgl kkqntllvll nhpqvpvamq ngilqlvthv dskvtsclql galytleare
      121 cndsptsfvs fhgedfdhhw qpcwtasswi kasgstmsst lvvdkncwrv pelfsgaat
//
LOCUS       XP_077501701             121 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112753 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501701
VERSION     XP_077501701.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645575.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..121
                     /product="uncharacterized protein LOC144112753 isoform X2"
                     /calculated_mol_wt=13137
     CDS             1..121
                     /gene="LOC144112753"
                     /coded_by="XM_077645575.1:100..465"
                     /db_xref="GeneID:144112753"
ORIGIN      
        1 mcsaivlsll spvqgirela vslaqaalns shkgcsrphl lfkeidqhaa evaadteell
       61 elivtwshgl kkqntllvll nhpqvpvamq ngilqlvthv dsktaqaata arrsnfrrde
      121 w
//
LOCUS       XP_077501702             291 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112757 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501702
VERSION     XP_077501702.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645576.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..291
                     /product="uncharacterized protein LOC144112757 isoform X1"
                     /calculated_mol_wt=32392
     CDS             1..291
                     /gene="LOC144112757"
                     /coded_by="XM_077645576.1:166..1041"
                     /db_xref="GeneID:144112757"
ORIGIN      
        1 mefsktalty rtqhhagaag prrpvgetay hdcrriegly syptgiitll lhllssiiep
       61 tlpqpevayq plipdikhvt ckriatlprr lslfpllarr dllstsreka wsrgspgarw
      121 ssgrrrghar tqichgdrcr yrpatvcelt avseeprgrs splsterppl hipgeglvar
      181 fswgrhaqaq tqichgdccc ycpatvcelt aagrergatr gqvplllhek gggdvveely
      241 pprafpamvr egpavhdrpe qrwwkeqlsn ahnpfcchar lstskqhvsn v
//
LOCUS       XP_077501703             284 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112757 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501703
VERSION     XP_077501703.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645577.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..284
                     /product="uncharacterized protein LOC144112757 isoform X2"
                     /calculated_mol_wt=31718
     CDS             1..284
                     /gene="LOC144112757"
                     /coded_by="XM_077645577.1:166..1020"
                     /db_xref="GeneID:144112757"
ORIGIN      
        1 mefsktalty rtqhhagaag prrpvgetay hdcrriegly syptgiitll lhllssiiep
       61 tlpqpevayq plipdikhvt ckriatlprr lslfpllarr dllstsreka wsrgspgarw
      121 ssgrrrghar tqichgdrcr yrpatvcelt avseeprgrs splsterppl hipgeglvar
      181 fswgrhaqaq tqichgdccc ycpatvcelt aagrergatr gqvplllhek gggdvveely
      241 pprafpamvr egpavhdrpe qrwwkeqlsv pmcnpwflrl lgmv
//
LOCUS       XP_077501704             278 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112757 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501704
VERSION     XP_077501704.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645578.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..278
                     /product="uncharacterized protein LOC144112757 isoform X3"
                     /calculated_mol_wt=30913
     CDS             1..278
                     /gene="LOC144112757"
                     /coded_by="XM_077645578.1:213..1049"
                     /db_xref="GeneID:144112757"
ORIGIN      
        1 mefsktalty rtqhhagaag prrpvgetay hdcrriegly syptgiitll lhllssiiep
       61 tlpqpevayq plipdikhvt ckriarrdll stsrekawsr gspgarwssg rrrghartqi
      121 chgdrcryrp atvceltavs eeprgrsspl sterpplhip geglvarfsw grhaqaqtqi
      181 chgdcccycp atvceltaag rergatrgqv plllhekggg dvveelyppr afpamvregp
      241 avhdrpeqrw wkeqlsnahn pfccharlst skqhvsnv
//
LOCUS       XP_077501705             271 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112757 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077501705
VERSION     XP_077501705.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645579.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..271
                     /product="uncharacterized protein LOC144112757 isoform X4"
                     /calculated_mol_wt=30116
     CDS             1..271
                     /gene="LOC144112757"
                     /coded_by="XM_077645579.1:165..980"
                     /db_xref="GeneID:144112757"
ORIGIN      
        1 mefsktalty rtqhhagaag prrpvgetay hdcrriegly syptgiitll lhllssiiep
       61 tlpqpevayq plipdikhvt ckriatlprr lslfpllarr dllstsreka wsrgspgarw
      121 ssgrrrghar tqichgdrcr yrpatvcelt avseeprgrs splsterppl hipgeglvar
      181 fswgrhaqaq tqichgdccc ycpatvcelt aagrergatr gqvplllhek gggdvveely
      241 pprafpamvr egpavhdrpe qrwwkeqlsv g
//
LOCUS       XP_077501706             271 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112757 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077501706
VERSION     XP_077501706.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645580.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..271
                     /product="uncharacterized protein LOC144112757 isoform X4"
                     /calculated_mol_wt=30116
     CDS             1..271
                     /gene="LOC144112757"
                     /coded_by="XM_077645580.1:165..980"
                     /db_xref="GeneID:144112757"
ORIGIN      
        1 mefsktalty rtqhhagaag prrpvgetay hdcrriegly syptgiitll lhllssiiep
       61 tlpqpevayq plipdikhvt ckriatlprr lslfpllarr dllstsreka wsrgspgarw
      121 ssgrrrghar tqichgdrcr yrpatvcelt avseeprgrs splsterppl hipgeglvar
      181 fswgrhaqaq tqichgdccc ycpatvcelt aagrergatr gqvplllhek gggdvveely
      241 pprafpamvr egpavhdrpe qrwwkeqlsv g
//
LOCUS       XP_077501707             261 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112757 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077501707
VERSION     XP_077501707.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645581.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..261
                     /product="uncharacterized protein LOC144112757 isoform X5"
                     /calculated_mol_wt=29607
     CDS             1..261
                     /gene="LOC144112757"
                     /coded_by="XM_077645581.1:165..950"
                     /db_xref="GeneID:144112757"
ORIGIN      
        1 mefsktalty rtqhhagaag prrpvgetay hdcrriegly syptgiitll lhllssiiep
       61 tlpqpevayq plipdikhvt ckriatlprr lslfpllarr dllstsreka wsrgspgarw
      121 ssgrrrghar tqichgdrcr yrpatvcelt avseeprgqt thnhaskles pswrlfrart
      181 lfrpphlrrq gqvplllhek gggdvveely pprafpamvr egpavhdrpe qrwwkeqlsn
      241 ahnpfcchar lstskqhvsn v
//
LOCUS       XP_077501708             246 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112757 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077501708
VERSION     XP_077501708.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645582.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..246
                     /product="uncharacterized protein LOC144112757 isoform X6"
                     /calculated_mol_wt=27184
     CDS             1..246
                     /gene="LOC144112757"
                     /coded_by="XM_077645582.1:165..905"
                     /db_xref="GeneID:144112757"
ORIGIN      
        1 mefsktalty rtqhhagaag prrpvgetay hdcrriegly syptgiitll lhllssiiep
       61 tlpqpevayq plipdikhvt ckriatlprr lslfpllarr dllstsreka wsrgspgarw
      121 ssgrrrghar tqichgdrcr yrpatvcelt avseeprgrs splsterppl hipgeglvar
      181 fswgrhaqaq tqichgdccc ycpatvcelt aagrergatr gpstlsmnam dgwmdgrtdg
      241 rtsqkm
//
LOCUS       XP_077501709             288 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112760 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501709
VERSION     XP_077501709.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645583.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..288
                     /product="uncharacterized protein LOC144112760 isoform X1"
                     /calculated_mol_wt=31239
     CDS             1..288
                     /gene="LOC144112760"
                     /coded_by="XM_077645583.1:430..1296"
                     /db_xref="GeneID:144112760"
ORIGIN      
        1 mpavvpspaa vargrhspfq rrsfslwlsl frpsccrqpr tssgswpnri lchravttva
       61 strdpravcq paqvalnssh kgcsrphllf keidqhaaev aadteellel ivtwshglkk
      121 qntllvllnh pqvpvamqng ilqlvthvds kvskdlvkkl peenqamlld tlvdlaldtp
      181 psvqhsavsg alrkvcffgs llveplqrtg vslqcpartv reakkmrrtg psrvspvear
      241 phpagedpep kvdrrghaag watlcaaqek pgaghvppcr vhtctsac
//
LOCUS       XP_077501710             287 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112760 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501710
VERSION     XP_077501710.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645584.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..287
                     /product="uncharacterized protein LOC144112760 isoform X2"
                     /calculated_mol_wt=31083
     CDS             1..287
                     /gene="LOC144112760"
                     /coded_by="XM_077645584.1:430..1293"
                     /db_xref="GeneID:144112760"
ORIGIN      
        1 mpavvpspaa vargrhspfq rrsfslwlsl frpsccrqpr tssgswpnri lchravttva
       61 strdpravcq paqvalnssh kgcsrphllf keidqhaaev aadteellel ivtwshglkk
      121 qntllvllnh pqvpvamqng ilqlvthvds kvskdlvkkl peenqamlld tlvdlaldtp
      181 psvqhsavsg alrkvcffgs llveplqrtg vslqcpartv reakkmrtgp srvspvearp
      241 hpagedpepk vdrrghaagw atlcaaqekp gaghvppcrv htctsac
//
LOCUS       XP_077501711             114 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112761 [Amblyomma americanum].
ACCESSION   XP_077501711
VERSION     XP_077501711.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645585.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..114
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..114
                     /product="uncharacterized protein LOC144112761"
                     /calculated_mol_wt=12695
     CDS             1..114
                     /gene="LOC144112761"
                     /coded_by="XM_077645585.1:1..345"
                     /db_xref="GeneID:144112761"
ORIGIN      
        1 mttmgrqhrr llangaalpr vnarrsvdlh lvhglftsla rqtqtdvdek fardslqirl
       61 agtepelvqa vlalgkdlck fleqgvvlgt lrrllfepte qskkwfdlrt gvcv
//
LOCUS       XP_077501714             143 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112772 [Amblyomma americanum].
ACCESSION   XP_077501714
VERSION     XP_077501714.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645588.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..143
                     /product="uncharacterized protein LOC144112772"
                     /calculated_mol_wt=15717
     CDS             1..143
                     /gene="LOC144112772"
                     /coded_by="XM_077645588.1:658..1089"
                     /db_xref="GeneID:144112772"
ORIGIN      
        1 mwilrqqdda icatldllan ifgtvvehsv skgssqsisa adaevvqgaf lafflaralp
       61 gwpvavapgc qllcrrqqqs pggitlpkek lqslafavsa qllqtaedik wilveqnlvp
      121 scchycrqyk gserwlsawh rrh
//
LOCUS       XP_077501715             391 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112778 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501715
VERSION     XP_077501715.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645589.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..391
                     /product="uncharacterized protein LOC144112778 isoform X1"
                     /calculated_mol_wt=43680
     CDS             1..391
                     /gene="LOC144112778"
                     /coded_by="XM_077645589.1:213..1388"
                     /db_xref="GeneID:144112778"
ORIGIN      
        1 mefsktalty rtqhhagaag prrpvgetay hdcrriegly syptgiitll lhllssiiep
       61 tlpqpevayq plipdikhvt ckriatlprr lslfpllarr dllstsreka wsrsspgarw
      121 ssgrtrghar tqichgdrcr yrpatvcelt avseeprgrs splsterppl hipgeglvar
      181 fswgrhaqaq tqichgdccc ycpatvcelt aagrergatr gpstlsmnam dgwmdgrtdg
      241 rtdgrtskki rrptithqsw ralpgdffvl apssvpricv dkdrypfcft rkeeetswrs
      301 sirhalfqrw fvkvqpfttd qnsgggrnnf rnpmqadnpr sailhtavvs fetawnavrs
      361 hqttmvhlsa taeqgfvdld flpgaahawp l
//
LOCUS       XP_077501716             371 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112778 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501716
VERSION     XP_077501716.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645590.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..371
                     /product="uncharacterized protein LOC144112778 isoform X2"
                     /calculated_mol_wt=41780
     CDS             1..371
                     /gene="LOC144112778"
                     /coded_by="XM_077645590.1:213..1328"
                     /db_xref="GeneID:144112778"
ORIGIN      
        1 mefsktalty rtqhhagaag prrpvgetay hdcrriegly syptgiitll lhllssiiep
       61 tlpqpevayq plipdikhvt ckriatlprr lslfpllarr dllstsreka wsrsspgarw
      121 ssgrtrghar tqichgdrcr yrpatvcelt avseeprgrs splsterppl hipgeglvar
      181 fswgrhaqaq tqichgdccc ycpatvcelt aagrergatr gpstlsmnam dgwmdgrtdg
      241 rtdgrtskki rrptithqsw ralpgdffvl apssvpricv dkdrypfcft rkeeetswrs
      301 sirhalfqrw fvkvqpfttd qnsgggrnnf rwvkysnrcp cvthgffafl ewfsvlngkc
      361 pvyapvnism d
//
LOCUS       XP_077501717             364 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112778 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501717
VERSION     XP_077501717.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645591.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..364
                     /product="uncharacterized protein LOC144112778 isoform X3"
                     /calculated_mol_wt=40846
     CDS             1..364
                     /gene="LOC144112778"
                     /coded_by="XM_077645591.1:213..1307"
                     /db_xref="GeneID:144112778"
ORIGIN      
        1 mefsktalty rtqhhagaag prrpvgetay hdcrriegly syptgiitll lhllssiiep
       61 tlpqpevayq plipdikhvt ckriatlprr lslfpllarr dllstsreka wsrsspgarw
      121 ssgrtrghar tqichgdrcr yrpatvcelt avseeprgrs splsterppl hipgeglvar
      181 fswgrhaqaq tqichgdccc ycpatvcelt aagrergatr gpstlsmnam dgwmdgrtdg
      241 rtdgrtskki rrptithqsw ralpgdffvl apssvpricv dkdrypfcft rkeeetswrs
      301 sirhalfqrw fvkvqpfttd qnsgggrnnf rcpcvthgff aflewfsvln gkcpvyapvn
      361 ismd
//
LOCUS       XP_077501718             356 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112778 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077501718
VERSION     XP_077501718.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645592.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..356
                     /product="uncharacterized protein LOC144112778 isoform X4"
                     /calculated_mol_wt=40077
     CDS             1..356
                     /gene="LOC144112778"
                     /coded_by="XM_077645592.1:213..1283"
                     /db_xref="GeneID:144112778"
ORIGIN      
        1 mefsktalty rtqhhagaag prrpvgetay hdcrriegly syptgiitll lhllssiiep
       61 tlpqpevayq plipdikhvt ckriatlprr lslfpllarr dllstsreka wsrsspgarw
      121 ssgrtrghar tqichgdrcr yrpatvcelt avseeprgrs splsterppl hipgeglvar
      181 fswgrhaqaq tqichgdccc ycpatvcelt aagrergatr gpstlsmnam dgwmdgrtdg
      241 rtdgrtskki rrptithqsw ralpgdffvl apssvpricv dkdrypfcft rkeeetswrs
      301 sirhalfqrw fvkvqpfttd qnsgggrnnf rwvkysnrmp ithfavtlgf prasnm
//
LOCUS       XP_077501719             351 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112778 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077501719
VERSION     XP_077501719.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645593.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..351
                     /product="uncharacterized protein LOC144112778 isoform X5"
                     /calculated_mol_wt=39054
     CDS             1..351
                     /gene="LOC144112778"
                     /coded_by="XM_077645593.1:213..1268"
                     /db_xref="GeneID:144112778"
ORIGIN      
        1 mefsktalty rtqhhagaag prrpvgetay hdcrriegly syptgiitll lhllssiiep
       61 tlpqpevayq plipdikhvt ckriatlprr lslfpllarr dllstsreka wsrsspgarw
      121 ssgrtrghar tqichgdrcr yrpatvcelt avseeprgrs splsterppl hipgeglvar
      181 fswgrhaqaq tqichgdccc ycpatvcelt aagrergatr gpstlsmnam dgwmdgrtdg
      241 rtdgrtskki rrptithqsw ralpgdffvl apssvpricv dkvplllhek gggdvveely
      301 pprafpamvr egpaihdrpe qrwwkeqlsn ahnpfcchar lstskqhvsn v
//
LOCUS       XP_077501720             349 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112778 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077501720
VERSION     XP_077501720.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645594.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..349
                     /product="uncharacterized protein LOC144112778 isoform X6"
                     /calculated_mol_wt=39143
     CDS             1..349
                     /gene="LOC144112778"
                     /coded_by="XM_077645594.1:213..1262"
                     /db_xref="GeneID:144112778"
ORIGIN      
        1 mefsktalty rtqhhagaag prrpvgetay hdcrriegly syptgiitll lhllssiiep
       61 tlpqpevayq plipdikhvt ckriatlprr lslfpllarr dllstsreka wsrsspgarw
      121 ssgrtrghar tqichgdrcr yrpatvcelt avseeprgrs splsterppl hipgeglvar
      181 fswgrhaqaq tqichgdccc ycpatvcelt aagrergatr gpstlsmnam dgwmdgrtdg
      241 rtdgrtskki rrptithqsw ralpgdffvl apssvpricv dkdrypfcft rkeeetswrs
      301 sirhalfqrw fvkvqpfttd qnsgggrnnf rmpithfavt lgfprasnm
//
LOCUS       XP_077501721             344 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112778 isoform X7 [Amblyomma
            americanum].
ACCESSION   XP_077501721
VERSION     XP_077501721.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645595.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..344
                     /product="uncharacterized protein LOC144112778 isoform X7"
                     /calculated_mol_wt=38379
     CDS             1..344
                     /gene="LOC144112778"
                     /coded_by="XM_077645595.1:213..1247"
                     /db_xref="GeneID:144112778"
ORIGIN      
        1 mefsktalty rtqhhagaag prrpvgetay hdcrriegly syptgiitll lhllssiiep
       61 tlpqpevayq plipdikhvt ckriatlprr lslfpllarr dllstsreka wsrsspgarw
      121 ssgrtrghar tqichgdrcr yrpatvcelt avseeprgrs splsterppl hipgeglvar
      181 fswgrhaqaq tqichgdccc ycpatvcelt aagrergatr gpstlsmnam dgwmdgrtdg
      241 rtdgrtskki rrptithqsw ralpgdffvl apssvpricv dkvplllhek gggdvveely
      301 pprafpamvr egpaihdrpe qrwwkeqlsv pmcnpwflrl lgmv
//
LOCUS       XP_077501722             222 aa            linear   INV 25-NOV-2025
DEFINITION  HEAT repeat-containing protein 1-like [Amblyomma americanum].
ACCESSION   XP_077501722
VERSION     XP_077501722.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645596.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..222
                     /product="HEAT repeat-containing protein 1-like"
                     /calculated_mol_wt=24150
     CDS             1..222
                     /gene="LOC144112779"
                     /coded_by="XM_077645596.1:204..872"
                     /db_xref="GeneID:144112779"
ORIGIN      
        1 mrcsreawsw acppmpstyl hqcvltalqa hgllaprqel aslvkclris qtaqtqqlvl
       61 gllslaalhy peevlhnvts vftykgasll wqvdncsfqt vmptvktvmp allqactges
      121 ksaeaaesav asvtrvfvgp fpdipehwwl slfaklattl gagdhlwila aqmaehqvtk
      181 mggtdvdevt fvrymsgrmk frltlrcscc rtlsgadvpp ag
//
LOCUS       XP_077501723             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112780 [Amblyomma americanum].
ACCESSION   XP_077501723
VERSION     XP_077501723.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645597.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144112780"
                     /calculated_mol_wt=14174
     CDS             1..127
                     /gene="LOC144112780"
                     /coded_by="XM_077645597.1:90..473"
                     /db_xref="GeneID:144112780"
ORIGIN      
        1 mpsavlwtfl ptylvqlwst rsaraaadqf rqlmqklfkv hflgglwllh ldasccavas
       61 sgrqgaslsl pkeklqslal avsaqllqta edikwilaeq nlvpscchyc rqykgsescl
      121 sawhrwh
//
LOCUS       XP_077501724             114 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112781 [Amblyomma americanum].
ACCESSION   XP_077501724
VERSION     XP_077501724.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645598.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..114
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..114
                     /product="uncharacterized protein LOC144112781"
                     /calculated_mol_wt=12695
     CDS             1..114
                     /gene="LOC144112781"
                     /coded_by="XM_077645598.1:1..345"
                     /db_xref="GeneID:144112781"
ORIGIN      
        1 mttmgrqhrr llangaalpr vnarrsvdlh lvhglftsla rqtqtdvdek fardslqirl
       61 agtepelvqa vlalgkdlck fleqgvvlgt lrrllfepte qskkwfdlrt gvcv
//
LOCUS       XP_077501725             214 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112783 [Amblyomma americanum].
ACCESSION   XP_077501725
VERSION     XP_077501725.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645599.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..214
                     /product="uncharacterized protein LOC144112783"
                     /calculated_mol_wt=22566
     CDS             1..214
                     /gene="LOC144112783"
                     /coded_by="XM_077645599.1:942..1586"
                     /db_xref="GeneID:144112783"
ORIGIN      
        1 mwilrqqdda icatldllan ifgtvvehsv skgssrsisa adaevvqgal pgwpvavapg
       61 cqllcrrqqr spggvtlpsk geasvsgsrc fgpaaadsrg hqvdlgrtes caivlsllsp
      121 vqgirevavs laqaalnssh kgcsrphllf keidqhaaev aadteellel ivtwshglkk
      181 qntllvllnh pqvpvamqng ilqlvthvds kvhd
//
LOCUS       XP_077501726             287 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112787 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501726
VERSION     XP_077501726.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645600.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..287
                     /product="uncharacterized protein LOC144112787 isoform X1"
                     /calculated_mol_wt=30598
     CDS             1..287
                     /gene="LOC144112787"
                     /coded_by="XM_077645600.1:763..1626"
                     /db_xref="GeneID:144112787"
ORIGIN      
        1 mwilrqqdda icatldllan ifgtvvehsv skgssrsisa adaevvqgal pgwpvavapg
       61 cqllcrrqqr spggvtlpsk geasvsgsrc fgpaaadsrg hqvdlgrtes caivlsllsp
      121 vqgirevavs laqaalnssh kgcsrphllf keidqhaaev aadteellel ivtwshglkk
      181 qntllvllnh pqvpvamqng ilqlvthvds kvtsclqlga lytlearecn dsptsfasfh
      241 gedfdhhwqp cwttsswika sgstmsstlv vdkncwrvpe lfsgaat
//
LOCUS       XP_077501727             214 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112787 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501727
VERSION     XP_077501727.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645601.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..214
                     /product="uncharacterized protein LOC144112787 isoform X2"
                     /calculated_mol_wt=22566
     CDS             1..214
                     /gene="LOC144112787"
                     /coded_by="XM_077645601.1:910..1554"
                     /db_xref="GeneID:144112787"
ORIGIN      
        1 mwilrqqdda icatldllan ifgtvvehsv skgssrsisa adaevvqgal pgwpvavapg
       61 cqllcrrqqr spggvtlpsk geasvsgsrc fgpaaadsrg hqvdlgrtes caivlsllsp
      121 vqgirevavs laqaalnssh kgcsrphllf keidqhaaev aadteellel ivtwshglkk
      181 qntllvllnh pqvpvamqng ilqlvthvds kvhd
//
LOCUS       XP_077501728            1129 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112791 [Amblyomma americanum].
ACCESSION   XP_077501728
VERSION     XP_077501728.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645602.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1129
                     /product="uncharacterized protein LOC144112791"
                     /calculated_mol_wt=128787
     Region          165..725
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1129
                     /gene="LOC144112791"
                     /coded_by="XM_077645602.1:346..3735"
                     /db_xref="GeneID:144112791"
ORIGIN      
        1 mmrltlsgpr hwaplcnlil rtrknaqvfy apvrellare lkavarpsvq dfgvvyalls
       61 vwsesfqvtd elamdaqscr etllelsara eecpraleea lfeiyfslsq gkvfwfrsal
      121 ehlyfryrah rqridvftgs viaqelpshm vkvrkviltp trrvymppql vcksrllqhc
      181 dpdyalrivv rdddcklisf slgackddfl savikpplss glkiggrrfm flgcstsqlr
      241 shgvwfyahd nqgrtaeqir dgigdlstiv svpkfmarlg qafsqslgll tvpsqntsve
      301 pdirhaapgr ssdgshreyi fsdgigrish gllrkvhhal eleegeepca vqiryggckg
      361 mllldptlsg crivfresmr kfhsdhsdly vlktskprvl ylnrpmitil eqsgikaevf
      421 lmlqnkilds fidsmmdphe aahvlrsyca lhlpykelag vgidltvepf fralvravnk
      481 kvlkelrtka rilvppnygr tmfgvldetg tleygqvfvq ysndmlqykv ndpatilegd
      541 vivtknpcmn pgdirkleav nvpqlhhvrd civfpqkger phpdemagsd ldgdeysvlw
      601 yedlifnnnc npmhyhsdpp kerkasigvq dmveffcqyi kgdkiglian ahlvwadild
      661 sginshrcre larkcavnld fakcgdlkgf qnsekppmyp dfmekldtkn tycsrkvlgq
      721 lyrnckkvel steclevvee slpdprlmle greqflkeat saykryakki rallksyrie
      781 teseafsgav sklskymken dptdmamvle sqvehvvrrt reeffaeqld eaheklkasa
      841 wyqvtyelqs seggiqsfpw vvcdvlmriv vntssclpvp asrnsfcqrl galllglphp
      901 gggdqdgtqh gdtqvltnll rlmydwidss reflfvknte elgvyksimr eacfkvsrsi
      961 srdmpphklv ilclrfacaw clkifqggsd geviskecrr ryrlghlali tlnrlsmsgn
     1021 laylrrapeg cpsteliriy inredeeffe ilrryediik kimmdwsgve diqcdlktdr
     1081 mdewflqlmv tgsrwalerl keivvypsfr evlllafere knaiggtle
//
LOCUS       XP_077501729             214 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112792 [Amblyomma americanum].
ACCESSION   XP_077501729
VERSION     XP_077501729.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645603.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..214
                     /product="uncharacterized protein LOC144112792"
                     /calculated_mol_wt=22586
     CDS             1..214
                     /gene="LOC144112792"
                     /coded_by="XM_077645603.1:2289..2933"
                     /db_xref="GeneID:144112792"
ORIGIN      
        1 mwilrqqdda icatfdllan ifgtvvehsv skgssrsvsa adaevvqgal pgwpvavapg
       61 cqllcrrqqr spggvtlpsk geasvsgsrc fgpaaadsrg hqvdlgrtes caivlsllsp
      121 vqgirevavs laqaalnssh kgcsrphllf keidqhaaev aadteellel ivtwshglkk
      181 qntllvllnh pqvpvamqng ilqlvthvds kvhd
//
LOCUS       XP_077501730             783 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112794 [Amblyomma americanum].
ACCESSION   XP_077501730
VERSION     XP_077501730.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645604.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..783
                     /product="uncharacterized protein LOC144112794"
                     /calculated_mol_wt=88932
     Region          312..406
                     /region_name="MULE"
                     /note="MULE transposase domain; pfam10551"
                     /db_xref="CDD:463145"
     CDS             1..783
                     /gene="LOC144112794"
                     /coded_by="XM_077645604.1:28..2379"
                     /db_xref="GeneID:144112794"
ORIGIN      
        1 masvfpnqse rqpehgdhdr rcgvcdkvft qrknmlqhmr mvhkletkga flhcdqcssl
       61 lstlenlvhh ynvahkfpae nlqlsfgndk efhdwkgqee asqvckfvis tgekrltngc
      121 trtyyacnrs gvarpgrgnk tramksqgsc rsgrtclsfl ivtrdegtvk vsyqrthygh
      181 dadvehlsmt dqerasiadd lskgvpmrti lkkiresvts kltplhltnr mdlhnikrqy
      241 nltvperchd ndavsvsiwv qsmrnkgqpv vrlykeqevv dptntfstkd falalmtepq
      301 eellkvlgtg tvcvdsthgt tgyqfelvtv lvldeygagl pmayficsti nektlsaffe
      361 slksstgals artfmtddas eyfkawstvm gppqhrllct whvdknwkrr isekikgkel
      421 avdvyhavrt llecpkkdev aellesflan kdprlqefiq yfrgnyahrl emwaysfrtg
      481 lgintnmyle rmhrtlkhsf legkknhrvd rlitalmdmt yeslmkrvqh ivkakrtskm
      541 srieknhrsa vaittpakrn edgtwtvqsq strgltyris kekgsaccpl kcsecmvcvh
      601 syscdceehr msltickhih cvviagapeg rdqkilkate avssnskase afhilesvqt
      661 aeastsmtks dlvlakmell keyvaeqeip qevhnkaskl ldpvlkiyeg tkkqemphts
      721 repankkieh qvrfhstkkq rlsssgtsfs kptelqkeni kkqilnsnde atviitspgh
      781 nyi
//
LOCUS       XP_077501731             616 aa            linear   INV 25-NOV-2025
DEFINITION  ki-ras-induced actin-interacting protein-IP3R-interacting domain
            olf186-M isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501731
VERSION     XP_077501731.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645605.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..616
                     /product="ki-ras-induced actin-interacting
                     protein-IP3R-interacting domain olf186-M isoform X1"
                     /calculated_mol_wt=67738
     Region          172..>251
                     /region_name="KRAP_IP3R_bind"
                     /note="Ki-ras-induced actin-interacting
                     protein-IP3R-interacting domain; pfam14722"
                     /db_xref="CDD:464280"
     CDS             1..616
                     /gene="olf186-M"
                     /coded_by="XM_077645605.1:704..2554"
                     /db_xref="GeneID:144112796"
ORIGIN      
        1 mgdtpspspr pqqgaenqrs rrglhklqdm kqlcpilelp kdlsrtvsea isvdvclver
       61 tnqkkviptg aetppkspts vqewvnslpa dephkaetsn mqpaelasfl hqdsgyqshl
      121 eghtleapis etqddnlslg aeartmdsas llqpkslqss gsgkamlael rskqssfnse
      181 tsgfstissm ssvesllear redpeellla lgfggpvrde npvsriperf lahpsqargv
      241 dmrrfvthqe qlsqrheagl papmhqsspp saisskiiea meqnqktrsf aataklarts
      301 slllssasrg ssdsilepan reflerqged kmsghkrlvl ghqtfsfdle sglmlltser
      361 rrmrwrwrra vriildkrrk ahdvvsdeal kypehahasl dgpagdsglg elisvnggsv
      421 dstssdycat snnghkdlsq kedllnaedd dddgdgegdl esipierhkr pllyrqstit
      481 eevgseqpad lldpsnqdgs eesgehtlte lsdlhralre ykakhlrcgs sladlgrktl
      541 qpasdleaiw qlreeisqev kradsmlssh emaallsqpc kdcvsytelk eklskvlslq
      601 kdlcsriaqi leqgsv
//
LOCUS       XP_077501732             588 aa            linear   INV 25-NOV-2025
DEFINITION  ki-ras-induced actin-interacting protein-IP3R-interacting domain
            olf186-M isoform X2 [Amblyomma americanum].
ACCESSION   XP_077501732
VERSION     XP_077501732.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645606.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..588
                     /product="ki-ras-induced actin-interacting
                     protein-IP3R-interacting domain olf186-M isoform X2"
                     /calculated_mol_wt=64654
     Region          144..>223
                     /region_name="KRAP_IP3R_bind"
                     /note="Ki-ras-induced actin-interacting
                     protein-IP3R-interacting domain; pfam14722"
                     /db_xref="CDD:464280"
     CDS             1..588
                     /gene="olf186-M"
                     /coded_by="XM_077645606.1:726..2492"
                     /db_xref="GeneID:144112796"
ORIGIN      
        1 mgdtpspspr pqqgaenqrs rrglhklqdm kqlcpilelp kgaetppksp tsvqewvnsl
       61 padephkaet snmqpaelas flhqdsgyqs hleghtleap isetqddnls lgaeartmds
      121 asllqpkslq ssgsgkamla elrskqssfn setsgfstis smssveslle arredpeell
      181 lalgfggpvr denpvsripe rflahpsqar gvdmrrfvth qeqlsqrhea glpapmhqss
      241 ppsaisskii eameqnqktr sfaataklar tsslllssas rgssdsilep anreflerqg
      301 edkmsghkrl vlghqtfsfd lesglmllts errrmrwrwr ravriildkr rkahdvvsde
      361 alkypehaha sldgpagdsg lgelisvngg svdstssdyc atsnnghkdl sqkedllnae
      421 dddddgdgeg dlesipierh krpllyrqst iteevgseqp adlldpsnqd gseesgehtl
      481 telsdlhral reykakhlrc gssladlgrk tlqpasdlea iwqlreeisq evkradsmls
      541 shemaallsq pckdcvsyte lkeklskvls lqkdlcsria qileqgsv
//
LOCUS       XP_077501733             212 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112797 [Amblyomma americanum].
ACCESSION   XP_077501733
VERSION     XP_077501733.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645607.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..212
                     /product="uncharacterized protein LOC144112797"
                     /calculated_mol_wt=23332
     CDS             1..212
                     /gene="LOC144112797"
                     /coded_by="XM_077645607.1:222..860"
                     /db_xref="GeneID:144112797"
ORIGIN      
        1 mdgvstpgrv rrtmdhhhqq qrrgtvrsmh gggrsrrpss avdskypsmd rscaiilmyg
       61 iyfcyvlacf gamlavaglm lhisseqpga hdkgkiiagt gigicavcfv iafycrsvrl
      121 deqimetike ttddrewsvf knpkagwwhm rygmadqssi rssatiqcnv rpdsaastss
      181 vvvyyqtkgl ettpseasps hsnrikdstd kl
//
LOCUS       XP_077501734            2094 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112799 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501734
VERSION     XP_077501734.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645608.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2094
                     /product="uncharacterized protein LOC144112799 isoform X1"
                     /calculated_mol_wt=229141
     Region          265..324
                     /region_name="Sushi"
                     /note="Sushi repeat (SCR repeat); pfam00084"
                     /db_xref="CDD:459664"
     Region          450..516
                     /region_name="CCP"
                     /note="Complement control protein (CCP) modules (aka short
                     consensus repeats SCRs or SUSHI repeats) have been
                     identified in several proteins of the complement system;
                     cd00033"
                     /db_xref="CDD:153056"
     Site            order(460,484)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     Region          660..718
                     /region_name="CCP"
                     /note="Domain abundant in complement control proteins;
                     SUSHI repeat; short complement-like repeat (SCR);
                     smart00032"
                     /db_xref="CDD:214478"
     Region          730..788
                     /region_name="CCP"
                     /note="Domain abundant in complement control proteins;
                     SUSHI repeat; short complement-like repeat (SCR);
                     smart00032"
                     /db_xref="CDD:214478"
     Site            order(740,764)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     Region          940..1006
                     /region_name="CCP"
                     /note="Complement control protein (CCP) modules (aka short
                     consensus repeats SCRs or SUSHI repeats) have been
                     identified in several proteins of the complement system;
                     cd00033"
                     /db_xref="CDD:153056"
     Site            order(950,974)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     Region          1075..1350
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     Region          1080..1146
                     /region_name="CCP"
                     /note="Complement control protein (CCP) modules (aka short
                     consensus repeats SCRs or SUSHI repeats) have been
                     identified in several proteins of the complement system;
                     cd00033"
                     /db_xref="CDD:153056"
     Site            order(1090,1114)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     Region          1290..1351
                     /region_name="CCP"
                     /note="Complement control protein (CCP) modules (aka short
                     consensus repeats SCRs or SUSHI repeats) have been
                     identified in several proteins of the complement system;
                     cd00033"
                     /db_xref="CDD:153056"
     Site            order(1300,1324)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     Region          1980..2024
                     /region_name="Ephrin_rec_like"
                     /note="Tyrosine-protein kinase ephrin type A/B
                     receptor-like; pfam07699"
                     /db_xref="CDD:429604"
     Region          2051..2080
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     CDS             1..2094
                     /gene="LOC144112799"
                     /coded_by="XM_077645608.1:163..6447"
                     /db_xref="GeneID:144112799"
ORIGIN      
        1 maprlwlwlh vatamvavsc tyhherhpht krshterhnf lgnfllngnd srtgsvprph
       61 advlpyraph edvarrrqrh rhrrrphgpr vpdiiysfgs qaapkllyrg kgyrvgpytg
      121 yvdvwtiprq arplslpppr sttstpplqq qrqrddfpeg tafarvliap ersnavepps
      181 clrcpkdrtv wvgpgfscae vpkprlrpcs rllslpgevh vtatmgpqpg slvmegvyqi
      241 sftvqapgmv pqschyklrv kvrrcpalrr pehgsarcsr grhwgsrcrf fcqpgytmqe
      301 rvatrcaihd gvrvswtphp epvcraknvs apdtptkpvv pstvvsarps tepsipptrp
      361 dtprttkrwk qlacrwpkap lhgwvscdrg serkarvvar egtrcrqgcr wdpqgvvthf
      421 lcvagswsgr kplacsgppv ssepptesgc eslpsvpnsv lecsavsntl grrpvrtvcr
      481 hvcddgfeis hsqlalarvt clpdgtwdnq lqpqcvksgc pspdpvpesl lectndanrl
      541 gnrptgtvck vvcregftlp ptqqsinhfi chedgtwnqt eqlqchengc pnpdfvpesl
      601 ldcsndagyl gnrpigttck imckegftlp lhleshsqfv cqengtwnqt arvechkngc
      661 pnpdpvpdss lecsnnadhl gnrpigtack vlcsegfslp ldlesrnhfi cqedgtwsqt
      721 awlechkngc pnpdpvpdss lecsndadhl gnrpigtack vvcsegfslp ldlesrshfi
      781 cqedgtwsqt arlechkdgc ksldpepnsa lecsadanhl gnqltgtvct vvcadgfslp
      841 sglqslnqfv cqedgswnkt adlecrknec pspdevpdss lecssdaddm gnrptgtick
      901 vvcadgftlp sslqphsqfi cqndgswnqt gqlichksgc pspdsvpdss lectndvndl
      961 gnhptgtmck vvctegftlp sslissgqfv cqedgtwnqt aqlecrqsgc snpdpvpdsa
     1021 lecshdanhl gnrptgttck vvcaegfslp sslqslnqlv cqedgmwsqk aqlqchrvtc
     1081 dkplpvlnsv vecsngtsvd gkwslgtvcr ltcdegfaip kgqealnpfv crgngtwslt
     1141 eplsceeagc kspalvedsv lscskesnsl grwpagtvcs ydckegysla ksqlpfnhfv
     1201 cfedgtwnqt edlhcrktgc edpsaaqfsm vncsgtvnsl grwpegtvcs hscedgfaip
     1261 qdqihlnlfi clddgswnqt eglhcretgc eepgqvrhsv lnctlstnal gkrppgtlcs
     1321 yscdegyalp ahleprnrfs csedgswdqk edllcnktgc erprllkssv ldcsesanvv
     1381 gnwpagticn hicdkgfvip qslqhlneiv clddgkwnet dnlecverrn pqlsqgcrde
     1441 vvvvdghnvs fpvaleaptf gafngtdatv ncsatevaay gthviscdaf dselgtnssc
     1501 afnvtalapg capfqsdpys rvscsadrna tggrlhpvgd vceyicqqae yvipqsklds
     1561 avkecrpdgs wsdedfhlce kqlppvvtsg ckddvihtdv lgnttvplpv frtasgqlvn
     1621 atctpeklld yreyqincva edrelrsstt csfkvivkgh sgprplrqpq ncvdvhevyd
     1681 typapvrppa feaasggpal vtcepatllg pgaynvtcta rdnktkaetq cayvvnarqi
     1741 eqfresqcls lrgpangdir ckrrdglitc hfscfpdyvm nttalpllar gfvvcdraqr
     1801 drfdfqeeyg ldalpactrl psptyfqgsl afhvglpncs leslhmdfmp kvnetlfdra
     1861 gdlcrqafcd itfvcetdvr rnasdpssas aamkvvwtvq arhnvnasnd ipeaeivieq
     1921 iqvlthyivt sdpgfyeeia kvggdlwqds frqsrfrlvc gsrgfaidad tahcvqcprg
     1981 tfeehgachl cplnhyqdse gqtscrecpk htfsfegakm vedctefagp dvpppardap
     2041 arqphhgrgp cdpnpcrhqg icylqsggya clcqqhyrge laeprpsaae tavs
//
LOCUS       XP_077501735            2086 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112799 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501735
VERSION     XP_077501735.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645609.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2086
                     /product="uncharacterized protein LOC144112799 isoform X2"
                     /calculated_mol_wt=228454
     Region          265..324
                     /region_name="Sushi"
                     /note="Sushi repeat (SCR repeat); pfam00084"
                     /db_xref="CDD:459664"
     Region          450..516
                     /region_name="CCP"
                     /note="Complement control protein (CCP) modules (aka short
                     consensus repeats SCRs or SUSHI repeats) have been
                     identified in several proteins of the complement system;
                     cd00033"
                     /db_xref="CDD:153056"
     Site            order(460,484)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     Region          660..718
                     /region_name="CCP"
                     /note="Domain abundant in complement control proteins;
                     SUSHI repeat; short complement-like repeat (SCR);
                     smart00032"
                     /db_xref="CDD:214478"
     Region          730..788
                     /region_name="CCP"
                     /note="Domain abundant in complement control proteins;
                     SUSHI repeat; short complement-like repeat (SCR);
                     smart00032"
                     /db_xref="CDD:214478"
     Site            order(740,764)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     Region          940..1006
                     /region_name="CCP"
                     /note="Complement control protein (CCP) modules (aka short
                     consensus repeats SCRs or SUSHI repeats) have been
                     identified in several proteins of the complement system;
                     cd00033"
                     /db_xref="CDD:153056"
     Site            order(950,974)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     Region          1075..1350
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     Region          1080..1146
                     /region_name="CCP"
                     /note="Complement control protein (CCP) modules (aka short
                     consensus repeats SCRs or SUSHI repeats) have been
                     identified in several proteins of the complement system;
                     cd00033"
                     /db_xref="CDD:153056"
     Site            order(1090,1114)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     Region          1290..1351
                     /region_name="CCP"
                     /note="Complement control protein (CCP) modules (aka short
                     consensus repeats SCRs or SUSHI repeats) have been
                     identified in several proteins of the complement system;
                     cd00033"
                     /db_xref="CDD:153056"
     Site            order(1300,1324)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     Region          1980..2024
                     /region_name="Ephrin_rec_like"
                     /note="Tyrosine-protein kinase ephrin type A/B
                     receptor-like; pfam07699"
                     /db_xref="CDD:429604"
     Region          2052..2083
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     CDS             1..2086
                     /gene="LOC144112799"
                     /coded_by="XM_077645609.1:163..6423"
                     /db_xref="GeneID:144112799"
ORIGIN      
        1 maprlwlwlh vatamvavsc tyhherhpht krshterhnf lgnfllngnd srtgsvprph
       61 advlpyraph edvarrrqrh rhrrrphgpr vpdiiysfgs qaapkllyrg kgyrvgpytg
      121 yvdvwtiprq arplslpppr sttstpplqq qrqrddfpeg tafarvliap ersnavepps
      181 clrcpkdrtv wvgpgfscae vpkprlrpcs rllslpgevh vtatmgpqpg slvmegvyqi
      241 sftvqapgmv pqschyklrv kvrrcpalrr pehgsarcsr grhwgsrcrf fcqpgytmqe
      301 rvatrcaihd gvrvswtphp epvcraknvs apdtptkpvv pstvvsarps tepsipptrp
      361 dtprttkrwk qlacrwpkap lhgwvscdrg serkarvvar egtrcrqgcr wdpqgvvthf
      421 lcvagswsgr kplacsgppv ssepptesgc eslpsvpnsv lecsavsntl grrpvrtvcr
      481 hvcddgfeis hsqlalarvt clpdgtwdnq lqpqcvksgc pspdpvpesl lectndanrl
      541 gnrptgtvck vvcregftlp ptqqsinhfi chedgtwnqt eqlqchengc pnpdfvpesl
      601 ldcsndagyl gnrpigttck imckegftlp lhleshsqfv cqengtwnqt arvechkngc
      661 pnpdpvpdss lecsnnadhl gnrpigtack vlcsegfslp ldlesrnhfi cqedgtwsqt
      721 awlechkngc pnpdpvpdss lecsndadhl gnrpigtack vvcsegfslp ldlesrshfi
      781 cqedgtwsqt arlechkdgc ksldpepnsa lecsadanhl gnqltgtvct vvcadgfslp
      841 sglqslnqfv cqedgswnkt adlecrknec pspdevpdss lecssdaddm gnrptgtick
      901 vvcadgftlp sslqphsqfi cqndgswnqt gqlichksgc pspdsvpdss lectndvndl
      961 gnhptgtmck vvctegftlp sslissgqfv cqedgtwnqt aqlecrqsgc snpdpvpdsa
     1021 lecshdanhl gnrptgttck vvcaegfslp sslqslnqlv cqedgmwsqk aqlqchrvtc
     1081 dkplpvlnsv vecsngtsvd gkwslgtvcr ltcdegfaip kgqealnpfv crgngtwslt
     1141 eplsceeagc kspalvedsv lscskesnsl grwpagtvcs ydckegysla ksqlpfnhfv
     1201 cfedgtwnqt edlhcrktgc edpsaaqfsm vncsgtvnsl grwpegtvcs hscedgfaip
     1261 qdqihlnlfi clddgswnqt eglhcretgc eepgqvrhsv lnctlstnal gkrppgtlcs
     1321 yscdegyalp ahleprnrfs csedgswdqk edllcnktgc erprllkssv ldcsesanvv
     1381 gnwpagticn hicdkgfvip qslqhlneiv clddgkwnet dnlecverrn pqlsqgcrde
     1441 vvvvdghnvs fpvaleaptf gafngtdatv ncsatevaay gthviscdaf dselgtnssc
     1501 afnvtalapg capfqsdpys rvscsadrna tggrlhpvgd vceyicqqae yvipqsklds
     1561 avkecrpdgs wsdedfhlce kqlppvvtsg ckddvihtdv lgnttvplpv frtasgqlvn
     1621 atctpeklld yreyqincva edrelrsstt csfkvivkgh sgprplrqpq ncvdvhevyd
     1681 typapvrppa feaasggpal vtcepatllg pgaynvtcta rdnktkaetq cayvvnarqi
     1741 eqfresqcls lrgpangdir ckrrdglitc hfscfpdyvm nttalpllar gfvvcdraqr
     1801 drfdfqeeyg ldalpactrl psptyfqgsl afhvglpncs leslhmdfmp kvnetlfdra
     1861 gdlcrqafcd itfvcetdvr rnasdpssas aamkvvwtvq arhnvnasnd ipeaeivieq
     1921 iqvlthyivt sdpgfyeeia kvggdlwqds frqsrfrlvc gsrgfaidad tahcvqcprg
     1981 tfeehgachl cplnhyqdse gqtscrecpk htfsfegakm vedctefagp dvpppardap
     2041 arqphhgrgp cdpnpcrhqg icylqsggya clcqqhyrgr hceqpa
//
LOCUS       XP_077501736            1870 aa            linear   INV 25-NOV-2025
DEFINITION  sushi, von Willebrand factor type A, EGF and pentraxin
            domain-containing protein 1-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077501736
VERSION     XP_077501736.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645610.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1870
                     /product="sushi, von Willebrand factor type A, EGF and
                     pentraxin domain-containing protein 1-like isoform X3"
                     /calculated_mol_wt=203655
     Region          41..100
                     /region_name="Sushi"
                     /note="Sushi repeat (SCR repeat); pfam00084"
                     /db_xref="CDD:459664"
     Region          226..292
                     /region_name="CCP"
                     /note="Complement control protein (CCP) modules (aka short
                     consensus repeats SCRs or SUSHI repeats) have been
                     identified in several proteins of the complement system;
                     cd00033"
                     /db_xref="CDD:153056"
     Site            order(236,260)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     Region          436..494
                     /region_name="CCP"
                     /note="Domain abundant in complement control proteins;
                     SUSHI repeat; short complement-like repeat (SCR);
                     smart00032"
                     /db_xref="CDD:214478"
     Region          506..564
                     /region_name="CCP"
                     /note="Domain abundant in complement control proteins;
                     SUSHI repeat; short complement-like repeat (SCR);
                     smart00032"
                     /db_xref="CDD:214478"
     Site            order(516,540)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     Region          716..776
                     /region_name="CCP"
                     /note="Complement control protein (CCP) modules (aka short
                     consensus repeats SCRs or SUSHI repeats) have been
                     identified in several proteins of the complement system;
                     cd00033"
                     /db_xref="CDD:153056"
     Site            order(726,750)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     Region          856..922
                     /region_name="CCP"
                     /note="Complement control protein (CCP) modules (aka short
                     consensus repeats SCRs or SUSHI repeats) have been
                     identified in several proteins of the complement system;
                     cd00033"
                     /db_xref="CDD:153056"
     Site            order(866,890)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     Region          1066..1127
                     /region_name="CCP"
                     /note="Complement control protein (CCP) modules (aka short
                     consensus repeats SCRs or SUSHI repeats) have been
                     identified in several proteins of the complement system;
                     cd00033"
                     /db_xref="CDD:153056"
     Site            order(1076,1100)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     Region          1756..1800
                     /region_name="Ephrin_rec_like"
                     /note="Tyrosine-protein kinase ephrin type A/B
                     receptor-like; pfam07699"
                     /db_xref="CDD:429604"
     Region          1827..1856
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     CDS             1..1870
                     /gene="LOC144112799"
                     /coded_by="XM_077645610.1:154..5766"
                     /db_xref="GeneID:144112799"
ORIGIN      
        1 mgpqpgslvm egvyqisftv qapgmvpqsc hyklrvkvrr cpalrrpehg sarcsrgrhw
       61 gsrcrffcqp gytmqervat rcaihdgvrv swtphpepvc raknvsapdt ptkpvvpstv
      121 vsarpsteps ipptrpdtpr ttkrwkqlac rwpkaplhgw vscdrgserk arvvaregtr
      181 crqgcrwdpq gvvthflcva gswsgrkpla csgppvssep ptesgceslp svpnsvlecs
      241 avsntlgrrp vrtvcrhvcd dgfeishsql alarvtclpd gtwdnqlqpq cvksgcpspd
      301 pvpesllect ndanrlgnrp tgtvckvvcr egftlpptqq sinhfiched gtwnqteqlq
      361 chengcpnpd fvpeslldcs ndagylgnrp igttckimck egftlplhle shsqfvcqen
      421 gtwnqtarve chkngcpnpd pvpdsslecs nnadhlgnrp igtackvlcs egfslpldle
      481 srnhficqed gtwsqtawle chkngcpnpd pvpdsslecs ndadhlgnrp igtackvvcs
      541 egfslpldle srshficqed gtwsqtarle chkdgcksld pepnsalecs adanhlgnql
      601 tgtvctvvca dgfslpsglq slnqfvcqed gswnktadle crknecpspd evpdsslecs
      661 sdaddmgnrp tgtickvvca dgftlpsslq phsqficqnd gswnqtgqli chksgcpspd
      721 svpdsslect ndvndlgnhp tgtmckvvct egftlpssli ssgqfvcqed gtwnqtaqle
      781 crqsgcsnpd pvpdsalecs hdanhlgnrp tgttckvvca egfslpsslq slnqlvcqed
      841 gmwsqkaqlq chrvtcdkpl pvlnsvvecs ngtsvdgkws lgtvcrltcd egfaipkgqe
      901 alnpfvcrgn gtwsltepls ceeagckspa lvedsvlscs kesnslgrwp agtvcsydck
      961 egyslaksql pfnhfvcfed gtwnqtedlh crktgcedps aaqfsmvncs gtvnslgrwp
     1021 egtvcshsce dgfaipqdqi hlnlficldd gswnqteglh cretgceepg qvrhsvlnct
     1081 lstnalgkrp pgtlcsyscd egyalpahle prnrfscsed gswdqkedll cnktgcerpr
     1141 llkssvldcs esanvvgnwp agticnhicd kgfvipqslq hlneivcldd gkwnetdnle
     1201 cverrnpqls qgcrdevvvv dghnvsfpva leaptfgafn gtdatvncsa tevaaygthv
     1261 iscdafdsel gtnsscafnv talapgcapf qsdpysrvsc sadrnatggr lhpvgdvcey
     1321 icqqaeyvip qskldsavke crpdgswsde dfhlcekqlp pvvtsgckdd vihtdvlgnt
     1381 tvplpvfrta sgqlvnatct peklldyrey qincvaedre lrssttcsfk vivkghsgpr
     1441 plrqpqncvd vhevydtypa pvrppafeaa sggpalvtce patllgpgay nvtctardnk
     1501 tkaetqcayv vnarqieqfr esqclslrgp angdirckrr dglitchfsc fpdyvmntta
     1561 lpllargfvv cdraqrdrfd fqeeygldal pactrlpspt yfqgslafhv glpncslesl
     1621 hmdfmpkvne tlfdragdlc rqafcditfv cetdvrrnas dpssasaamk vvwtvqarhn
     1681 vnasndipea eivieqiqvl thyivtsdpg fyeeiakvgg dlwqdsfrqs rfrlvcgsrg
     1741 faidadtahc vqcprgtfee hgachlcpln hyqdsegqts crecpkhtfs fegakmvedc
     1801 tefagpdvpp pardaparqp hhgrgpcdpn pcrhqgicyl qsggyaclcq qhyrgelaep
     1861 rpsaaetavs
//
LOCUS       XP_077501737             212 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112800 [Amblyomma americanum].
ACCESSION   XP_077501737
VERSION     XP_077501737.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645611.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..212
                     /product="uncharacterized protein LOC144112800"
                     /calculated_mol_wt=22898
     CDS             1..212
                     /gene="LOC144112800"
                     /coded_by="XM_077645611.1:134..772"
                     /db_xref="GeneID:144112800"
ORIGIN      
        1 mqlprwllac avvslaagvt gvvlrrwfrs yaigdilcis gmtlfttila sialkllgra
       61 lkrrvacsrf seilgvvtlr sgtqlvepqd vrllpagtlv cvvpptqpvf ypdnagdmlt
      121 ifedyaqfpq qpprmfdthr lspvstanha lerrvdpvaa ntsssepptt seededatss
      181 sdrppsyahl cgeppppykt dststlnddv vg
//
LOCUS       XP_077501738             212 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112800 [Amblyomma americanum].
ACCESSION   XP_077501738
VERSION     XP_077501738.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645612.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..212
                     /product="uncharacterized protein LOC144112800"
                     /calculated_mol_wt=22898
     CDS             1..212
                     /gene="LOC144112800"
                     /coded_by="XM_077645612.1:545..1183"
                     /db_xref="GeneID:144112800"
ORIGIN      
        1 mqlprwllac avvslaagvt gvvlrrwfrs yaigdilcis gmtlfttila sialkllgra
       61 lkrrvacsrf seilgvvtlr sgtqlvepqd vrllpagtlv cvvpptqpvf ypdnagdmlt
      121 ifedyaqfpq qpprmfdthr lspvstanha lerrvdpvaa ntsssepptt seededatss
      181 sdrppsyahl cgeppppykt dststlnddv vg
//
LOCUS       XP_077501739             269 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112801 [Amblyomma americanum].
ACCESSION   XP_077501739
VERSION     XP_077501739.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645613.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..269
                     /product="uncharacterized protein LOC144112801"
                     /calculated_mol_wt=27708
     CDS             1..269
                     /gene="LOC144112801"
                     /coded_by="XM_077645613.1:377..1186"
                     /db_xref="GeneID:144112801"
ORIGIN      
        1 mkpeggpqae ltygaiilvg vtavstglav fptsnkilga vligaglvti vaaallvdcl
       61 lkrfracrrv kppaydavas lhameageag pptepnsepl rrpcslsvha vtplchghfa
      121 vgsdlssgrs fsappseldl tagrlyrvhl rldsplssva drllhstvde prperadvsk
      181 plppapdass dgehpassea gkcaprapfc kppsydevla ddhrgrgdsv aspesqtlpa
      241 vggrmtlgrs sapeipcspl sigaapmgr
//
LOCUS       XP_077501740             269 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112801 [Amblyomma americanum].
ACCESSION   XP_077501740
VERSION     XP_077501740.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645614.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..269
                     /product="uncharacterized protein LOC144112801"
                     /calculated_mol_wt=27708
     CDS             1..269
                     /gene="LOC144112801"
                     /coded_by="XM_077645614.1:227..1036"
                     /db_xref="GeneID:144112801"
ORIGIN      
        1 mkpeggpqae ltygaiilvg vtavstglav fptsnkilga vligaglvti vaaallvdcl
       61 lkrfracrrv kppaydavas lhameageag pptepnsepl rrpcslsvha vtplchghfa
      121 vgsdlssgrs fsappseldl tagrlyrvhl rldsplssva drllhstvde prperadvsk
      181 plppapdass dgehpassea gkcaprapfc kppsydevla ddhrgrgdsv aspesqtlpa
      241 vggrmtlgrs sapeipcspl sigaapmgr
//
LOCUS       XP_077501741             269 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112801 [Amblyomma americanum].
ACCESSION   XP_077501741
VERSION     XP_077501741.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645615.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..269
                     /product="uncharacterized protein LOC144112801"
                     /calculated_mol_wt=27708
     CDS             1..269
                     /gene="LOC144112801"
                     /coded_by="XM_077645615.1:383..1192"
                     /db_xref="GeneID:144112801"
ORIGIN      
        1 mkpeggpqae ltygaiilvg vtavstglav fptsnkilga vligaglvti vaaallvdcl
       61 lkrfracrrv kppaydavas lhameageag pptepnsepl rrpcslsvha vtplchghfa
      121 vgsdlssgrs fsappseldl tagrlyrvhl rldsplssva drllhstvde prperadvsk
      181 plppapdass dgehpassea gkcaprapfc kppsydevla ddhrgrgdsv aspesqtlpa
      241 vggrmtlgrs sapeipcspl sigaapmgr
//
LOCUS       XP_077501742             269 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112801 [Amblyomma americanum].
ACCESSION   XP_077501742
VERSION     XP_077501742.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645616.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..269
                     /product="uncharacterized protein LOC144112801"
                     /calculated_mol_wt=27708
     CDS             1..269
                     /gene="LOC144112801"
                     /coded_by="XM_077645616.1:440..1249"
                     /db_xref="GeneID:144112801"
ORIGIN      
        1 mkpeggpqae ltygaiilvg vtavstglav fptsnkilga vligaglvti vaaallvdcl
       61 lkrfracrrv kppaydavas lhameageag pptepnsepl rrpcslsvha vtplchghfa
      121 vgsdlssgrs fsappseldl tagrlyrvhl rldsplssva drllhstvde prperadvsk
      181 plppapdass dgehpassea gkcaprapfc kppsydevla ddhrgrgdsv aspesqtlpa
      241 vggrmtlgrs sapeipcspl sigaapmgr
//
LOCUS       XP_077501743             156 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112803 [Amblyomma americanum].
ACCESSION   XP_077501743
VERSION     XP_077501743.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645617.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..156
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..156
                     /product="uncharacterized protein LOC144112803"
                     /calculated_mol_wt=16411
     CDS             1..156
                     /gene="LOC144112803"
                     /coded_by="XM_077645617.1:296..766"
                     /db_xref="GeneID:144112803"
ORIGIN      
        1 mvvpssgyia aplesvrldg atqqlpigpr gerrdsartt haalwfavva lvfvagmall
       61 asglhwwglc llgaallllv slcllpatag rqpeaaplag epvpvsmcah lqlrtisgna
      121 vpylleevtl advsrmyppp fppppyqaka tdprkk
//
LOCUS       XP_077501744             436 aa            linear   INV 25-NOV-2025
DEFINITION  protein kinase C iota type-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501744
VERSION     XP_077501744.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645618.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..436
                     /product="protein kinase C iota type-like isoform X1"
                     /calculated_mol_wt=48928
     Region          150..410
                     /region_name="STKc_AGC"
                     /note="Catalytic domain of AGC family Serine/Threonine
                     Kinases; cd05123"
                     /db_xref="CDD:270693"
     Site            order(150..154,158,171,173,206,222..223,225,229,231,268,
                     270,272..273,275,285..286,289,299..304,331,337,343)
                     /site_type="active"
                     /db_xref="CDD:270693"
     Site            order(150..154,158,171,173,206,223..225,229,268,270,
                     272..273,275,285..286)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270693"
     Site            order(154,229,231,268,270,272,289,299..304,331,337,343)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270693"
     Site            order(285..292,298..304)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270693"
     CDS             1..436
                     /gene="LOC144112804"
                     /coded_by="XM_077645618.1:297..1607"
                     /db_xref="GeneID:144112804"
ORIGIN      
        1 masrnvavke lvagrrkssd mmsplppdav laepeeqqqq ptcskvakkk kkkrpqddde
       61 hdqaaaevep katraksrsh dskaelkkke leqkqvvdgv gladkkvesf mdqagtstas
      121 apkkvvtdiw inpketvswn ldslvtlnvl grgsfgivyl vrhrvnrqla alkyirkknq
      181 npekerkrve veksvweavt dhpyvvtfra yfetakgwcf vmeylpegsi rdlvrkkgpf
      241 keetarllsa qvalaiqhvh ekgflhrdis ssnvlidsrg naklidfgls qigteawcrc
      301 gslaymapev irqelygmsa dwwsfgivlf vmlvgktpla iyaaekgvdl hlarrdiryr
      361 laliaplkip rtlsrsaqtl lreilkenpk rrlgcrpegg fddlmknpfy apidwsevsc
      421 gslpsttree gsnlst
//
LOCUS       XP_077501746             392 aa            linear   INV 25-NOV-2025
DEFINITION  protein kinase C iota type-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077501746
VERSION     XP_077501746.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645620.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..392
                     /product="protein kinase C iota type-like isoform X2"
                     /calculated_mol_wt=43909
     Region          147..365
                     /region_name="S_TKc"
                     /note="Serine/Threonine protein kinases, catalytic domain;
                     smart00220"
                     /db_xref="CDD:214567"
     Site            order(150..153,156,158,171,173,206,222..225,268,272..273,
                     275,285..286)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..392
                     /gene="LOC144112804"
                     /coded_by="XM_077645620.1:297..1475"
                     /db_xref="GeneID:144112804"
ORIGIN      
        1 masrnvavke lvagrrkssd mmsplppdav laepeeqqqq ptcskvakkk kkkrpqddde
       61 hdqaaaevep katraksrsh dskaelkkke leqkqvvdgv gladkkvesf mdqagtstas
      121 apkkvvtdiw inpketvswn ldslvtlnvl grgsfgivyl vrhrvnrqla alkyirkknq
      181 npekerkrve veksvweavt dhpyvvtfra yfetakgwcf vmeylpegsi rdlvrkkgpf
      241 keetarllsa qvalaiqhvh ekgflhrdis ssnvlidsrg naklidfgls qigteawcrc
      301 gslaymapev irqelyvali aplkiprtls rsaqtllrei lkenpkrrlg crpeggfddl
      361 mknpfyapid wsevscgslp sttreegsnl st
//
LOCUS       XP_077501747             963 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112805 [Amblyomma americanum].
ACCESSION   XP_077501747
VERSION     XP_077501747.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645621.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..963
                     /product="uncharacterized protein LOC144112805"
                     /calculated_mol_wt=107088
     CDS             1..963
                     /gene="LOC144112805"
                     /coded_by="XM_077645621.1:5261..8152"
                     /db_xref="GeneID:144112805"
ORIGIN      
        1 mttsttafvt valllllksg slmgrgpgpp essqlaytmd vnpvhpyqap vyymrnfisq
       61 lesrprdinf flelarepng fldildnyqh gismvvqign aimavvaigg vvvvaarwag
      121 ycggdniqev tpfsktayti tmsmtlflaa vdfvlafmly qsstdlstai dalpvvkema
      181 akdlaafvtn tinqkmdaae rkkrrrleel eddfqkafsr flkarlhndl mlhnalnvrd
      241 caqsvreigm lfvkdgaqkl grrllqtsks larvhdyrer lcdrvaslvg nstaigrrki
      301 vhrlqkevhe smqasaqael ketlngldra fsrlmsygsk tgwwdrfrst amfgivpfff
      361 iliiavlvpf tiaamtqfnf eeepeernir shvsgvaliy cafflyifvv lalyvalqlf
      421 pygavgecyl cdsyrqgsft vlnmlavrlw plnerasmfr gihpsevlsk cshgdatlad
      481 lsipaevrqd mdaghrtska psldmepfkl aqrmpsflvh drhvamlrad dvnvttfits
      541 lrtslskers lsestrrkts lflkkwerld ledaterlav tsyrrmlltm lpkyykeyfh
      601 gptidrhign cgpvynvvdk tmsatcdgfv dsllafvfll vvaavvstll avmaakggkk
      661 nkkgkkkgkk gkkkgkkkgk kkkkkkkkkk gkkdgedsks ekssdsslsk tttpeaaksv
      721 tpaptppktp prtasedavi ritttrtgds pagsvdqtdf leiripnqqe lapppvvvqp
      781 aktpthtvet lhiqipqgqq vaqpvavvsp pspqkqtsti eirvpqklmq pkqpqivavp
      841 tpanrrqvqr vvslnlvgnq nalgsrrsts qtsvvqlspt rqvqvlrqps grqlspvqwp
      901 wnywypywgg ggggqpmasv vtestssvev ieraripava rrvqsgpvva rraypyyayp
      961 qlw
//
LOCUS       XP_077501748             102 aa            linear   INV 25-NOV-2025
DEFINITION  isoinhibitor K-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501748
VERSION     XP_077501748.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645622.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..102
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..102
                     /product="isoinhibitor K-like isoform X1"
                     /calculated_mol_wt=11475
     Region          24..74
                     /region_name="Kunitz-type"
                     /note="Kunitz/Bovine pancreatic trypsin inhibitor (BPTI)
                     domain; cl00101"
                     /db_xref="CDD:444694"
     Site            order(30..34,36..38,53,57)
                     /site_type="other"
                     /note="putative serine protease binding site [polypeptide
                     binding]"
                     /db_xref="CDD:438679"
     CDS             1..102
                     /gene="LOC144112806"
                     /coded_by="XM_077645622.1:99..407"
                     /db_xref="GeneID:144112806"
ORIGIN      
        1 mkslvcllli slslvchaah kkacnlkkdp gncedastkw yydsktnack lfvyggcdgn
       61 dnrfdteakc kaecvrclhe ltaeyrlrwk dfvrllpkqa pc
//
LOCUS       XP_077501749              83 aa            linear   INV 25-NOV-2025
DEFINITION  isoinhibitor K-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077501749
VERSION     XP_077501749.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645623.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..83
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..83
                     /product="isoinhibitor K-like isoform X2"
                     /calculated_mol_wt=9015
     Region          24..74
                     /region_name="Kunitz-type"
                     /note="Kunitz/Bovine pancreatic trypsin inhibitor (BPTI)
                     domain; cl00101"
                     /db_xref="CDD:444694"
     Site            order(30..34,36..38,53,57)
                     /site_type="other"
                     /note="putative serine protease binding site [polypeptide
                     binding]"
                     /db_xref="CDD:438679"
     CDS             1..83
                     /gene="LOC144112806"
                     /coded_by="XM_077645623.1:99..350"
                     /db_xref="GeneID:144112806"
ORIGIN      
        1 mkslvcllli slslvchaah kkacnlkkdp gncedastkw yydsktnack lfvyggcdgn
       61 dnrfdteakc kaecvhprkt tsg
//
LOCUS       XP_077501750            1005 aa            linear   INV 25-NOV-2025
DEFINITION  zinc metalloproteinase-disintegrin-like protein F1 [Amblyomma
            americanum].
ACCESSION   XP_077501750
VERSION     XP_077501750.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645624.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1005
                     /product="zinc metalloproteinase-disintegrin-like protein
                     F1"
                     /calculated_mol_wt=110968
     Region          45..162
                     /region_name="Pep_M12B_propep"
                     /note="Reprolysin family propeptide; pfam01562"
                     /db_xref="CDD:460254"
     Region          210..407
                     /region_name="ZnMc_adamalysin_II_like"
                     /note="Zinc-dependent metalloprotease; adamalysin_II_like
                     subfamily. Adamalysin II is a snake venom zinc
                     endopeptidase. This subfamily contains other snake venom
                     metalloproteinases, as well as membrane-anchored
                     metalloproteases belonging to the ADAM family; cd04269"
                     /db_xref="CDD:239797"
     Site            order(346..347,350,356)
                     /site_type="active"
                     /db_xref="CDD:239797"
     Region          423..499
                     /region_name="DISIN"
                     /note="Homologues of snake disintegrins; smart00050"
                     /db_xref="CDD:214490"
     Region          501..645
                     /region_name="ACR"
                     /note="ADAM Cysteine-Rich Domain; smart00608"
                     /db_xref="CDD:214743"
     CDS             1..1005
                     /gene="LOC144112808"
                     /coded_by="XM_077645624.1:91..3108"
                     /db_xref="GeneID:144112808"
ORIGIN      
        1 mwgrlksswn ssfivllavl dfavcavlee etagarqdfl ryervhpkvf shgrrrrdvl
       61 slkntthqdv lqvsfkafgq pytldlrmkk dllplnyfek hhensshvid hpmkrtnkhc
      121 yyhgtvrgqk nswvavstcn glsgvvfdgt elhyihpdlp ehlffkasdm kaktwrcgfn
      181 dsqdlwgnah krakrsvviq ppyksnarsr yvellivndn kefikmkrnk eavferskei
      241 anivnalyap lnifialvgv ivwtehdeiv mssdgdatlt kflqyrrerl arehpndnaq
      301 litdivldss vvgkalkgpi ctyeysggvn kdhhevvgvv attvahelgh nfgmehddkd
      361 tkchcpekrc imasstsnpp spsqwsscsk qylqssfeqg mdfclwnlpe dimgpvcgng
      421 fleegeqcdc gpmefcknpc cdaatckfkp haacaigacc dvstcqvvka atlcrdtvte
      481 cdlpeycdgv sefcpvdvfv qdgtecgggk aycfgkqcrs hddqcqllwg ssgrmadqrc
      541 fernddgevn ancgynrlnk tykkcnkedv mcgtlhcvhl nerlefgies aavqskfyid
      601 endkrytcrs aivdlglfta dvgqspngak cgtnkacmnq kcvplekiyg vkcpydcynn
      661 gvcnsrgnch cmvgyappyc nypgpggssd sgpasdpsag ysfmvsmyii flcivplavi
      721 tsfsiyyfrr hlktwwmtka rkaaiksraq qvgldrrggr plskfnidae aikalnisap
      781 vtqpmvpqqp lapsappmpp sqhqkphrsv srergsfrnv disgpvlqss snshvaptrp
      841 aparpaparp apgrpagvqr apscpsqgsm rgrprvarsa sqrsngprpa hpppprppap
      901 pdgplyddca tslefagtpl afvhdepnsa qnrvvnrplp arptpassqp tpvnsrptpv
      961 nsrstvggva alaqkferna tessqphhgr vqptpgqrsy tttls
//
LOCUS       XP_077501751             713 aa            linear   INV 25-NOV-2025
DEFINITION  propionyl-CoA carboxylase alpha chain, mitochondrial-like
            [Amblyomma americanum].
ACCESSION   XP_077501751
VERSION     XP_077501751.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645625.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..713
                     /product="propionyl-CoA carboxylase alpha chain,
                     mitochondrial-like"
                     /calculated_mol_wt=78625
     Region          47..504
                     /region_name="PccA"
                     /note="Acetyl/propionyl-CoA carboxylase, alpha subunit
                     [Lipid transport and metabolism]; COG4770"
                     /db_xref="CDD:443802"
     Region          <498..713
                     /region_name="PRK12999"
                     /note="pyruvate carboxylase; Reviewed; cl39082"
                     /db_xref="CDD:476865"
     Region          647..712
                     /region_name="biotinyl_domain"
                     /note="The biotinyl-domain or biotin carboxyl carrier
                     protein (BCCP) domain is present in all biotin-dependent
                     enzymes, such as acetyl-CoA carboxylase, pyruvate
                     carboxylase, propionyl-CoA carboxylase, methylcrotonyl-CoA
                     carboxylase, geranyl-CoA carboxylase; cd06850"
                     /db_xref="CDD:133459"
     Site            order(669,678..680,687)
                     /site_type="active"
                     /note="carboxyltransferase (CT) interaction site [active]"
                     /db_xref="CDD:133459"
     Site            679
                     /site_type="other"
                     /note="biotinylation site [posttranslational
                     modification]"
                     /db_xref="CDD:133459"
     CDS             1..713
                     /gene="LOC144112809"
                     /coded_by="XM_077645625.1:72..2213"
                     /db_xref="GeneID:144112809"
ORIGIN      
        1 mapcsvvtsn llsllgrhrl rlcaplvrnq swtsiyttdp idpkedkfkk ilianrgeia
       61 crvihtckmm giktvavhsd vdsrnlhvkl adeaycigpa ptsqsylrvd aildvvkksg
      121 adavhpgygf lsenmdfaak lteagvtfig pnskaismmg dkieskrian aakvncipgf
      181 dgvvqtpeec vkiaqtigyp vmikasaggg gkgmriawnd keaidgfrfs seeakssfgd
      241 drllvekfid kprhievqlm cdkhgnavyl nerecsiqrr nqkvieecps tfldadtrka
      301 mgeqavqlal avgydsagtv eflvdskknf yflemntrlq vehpitecvt gvdivhqmir
      361 vakghplris qkdiplrgws fecrvyaedp fkhfglpsig rlhryiepnh ipnvrcdsgi
      421 iegseisiyy dpmicklvty gddreaarht mlkaldayvi rgvthnisll ravlsephfi
      481 kgdidtgylp riypdgfkgk elsekeathf sclaaalflq dellarkfln ghkvataerl
      541 dlwdvevmvd gkktsvmlnh qgngnycvhv ggskfdvilp adlsqplvka svsgaeyilq
      601 ilqrdfsgnf kisfegtsln akvllqeaad flskmpekpk adtskiveap mpgvvkhvtv
      661 kvgdvvaehq evcvieamkm qnslvsaamg kiksvfvkpg qtvdegqllv ele
//
LOCUS       XP_077501752             659 aa            linear   INV 25-NOV-2025
DEFINITION  WD repeat-containing protein 91 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501752
VERSION     XP_077501752.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645626.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..659
                     /product="WD repeat-containing protein 91 isoform X1"
                     /calculated_mol_wt=73105
     Region          <343..652
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          347..384
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          390..433
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          438..485
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          491..526
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          532..575
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          583..624
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          627..652
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..659
                     /gene="LOC144112811"
                     /coded_by="XM_077645626.1:242..2221"
                     /db_xref="GeneID:144112811"
ORIGIN      
        1 maasqfaddl vrdylryrgf ftslkafdse vkadkdkgfr pdrvvehlss ciesmdlvgl
       61 rdtwshldsh ifrrleqsfi ptvrklemal lriyvvtcvt nsrqdkltef fdkmapelhg
      121 qaewkewfal pflksvednp nfqvyftrqw qemmiislrn flsvvyqsma lptllsyded
      181 mnkmqflqee neslkqqlaa grpeaceaad vlvapeepmd dfyviaqeap vaeggrrilp
      241 almrtfgniq tspvtgrklq tepkkpplee qnktkrppan stsenqtfsq dspaatslql
      301 kdvalsrlae kleratastf flksssgeed kllllnqeey nehqscithc kfsnsgtlas
      361 adtagvikvw ssnpsprtla tivsskssvt alewvhkhdh llvyglkqgn lrlydtkerr
      421 plfdivpesa svlkehsvac lacspvepvv vsslhsepsv mstapvpgkl llwdlkskql
      481 qghlkcsssv nccvfshngq lllcgtsggd iclfdvrthe citnwvahet etfsvqfsgd
      541 eatcftigae gtlrqwnvyk tgqkvaefpl hagcmgpsgf rpvgklfslg adsehiltca
      601 pssgivykee ngsftnvlcv gnhkshvvtv dwslavdcst catasldgsi tvatlfshv
//
LOCUS       XP_077501753             605 aa            linear   INV 25-NOV-2025
DEFINITION  WD repeat-containing protein 91 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077501753
VERSION     XP_077501753.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645627.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..605
                     /product="WD repeat-containing protein 91 isoform X2"
                     /calculated_mol_wt=66904
     Region          <289..598
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          293..330
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          336..379
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          384..431
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          437..472
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          478..521
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          529..570
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          573..598
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..605
                     /gene="LOC144112811"
                     /coded_by="XM_077645627.1:128..1945"
                     /db_xref="GeneID:144112811"
ORIGIN      
        1 mdlvglrdtw shldshifrr leqsfiptvr klemallriy vvtcvtnsrq dklteffdkm
       61 apelhgqaew kewfalpflk svednpnfqv yftrqwqemm iislrnflsv vyqsmalptl
      121 lsydedmnkm qflqeenesl kqqlaagrpe aceaadvlva peepmddfyv iaqeapvaeg
      181 grrilpalmr tfgniqtspv tgrklqtepk kppleeqnkt krppanstse nqtfsqdspa
      241 atslqlkdva lsrlaekler atastfflks ssgeedklll lnqeeynehq scithckfsn
      301 sgtlasadta gvikvwssnp sprtlativs skssvtalew vhkhdhllvy glkqgnlrly
      361 dtkerrplfd ivpesasvlk ehsvaclacs pvepvvvssl hsepsvmsta pvpgklllwd
      421 lkskqlqghl kcsssvnccv fshngqlllc gtsggdiclf dvrthecitn wvahetetfs
      481 vqfsgdeatc ftigaegtlr qwnvyktgqk vaefplhagc mgpsgfrpvg klfslgadse
      541 hiltcapssg ivykeengsf tnvlcvgnhk shvvtvdwsl avdcstcata sldgsitvat
      601 lfshv
//
LOCUS       XP_077501754             529 aa            linear   INV 25-NOV-2025
DEFINITION  tyrosine--tRNA ligase, cytoplasmic-like [Amblyomma americanum].
ACCESSION   XP_077501754
VERSION     XP_077501754.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645628.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..529
                     /product="tyrosine--tRNA ligase, cytoplasmic-like"
                     /calculated_mol_wt=58755
     Region          15..347
                     /region_name="nt_trans"
                     /note="nucleotidyl transferase superfamily; cl00015"
                     /db_xref="CDD:469580"
     Site            order(47,49..51,57,59..60,174,178,181,190,192..193,196,
                     222..223,230..233)
                     /site_type="active"
                     /db_xref="CDD:173902"
     Site            57..60
                     /site_type="other"
                     /note="HIGH motif"
                     /db_xref="CDD:173902"
     Site            order(84,87..88,137,139,141..143,145..146,148..151,
                     153..154,170,172..173,176..177)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:173902"
     Site            230..234
                     /site_type="other"
                     /note="KMSKS motif"
                     /db_xref="CDD:173902"
     Region          <342..529
                     /region_name="PLN02610"
                     /note="probable methionyl-tRNA synthetase"
                     /db_xref="CDD:215329"
     CDS             1..529
                     /gene="LOC144112812"
                     /coded_by="XM_077645628.1:129..1718"
                     /db_xref="GeneID:144112812"
ORIGIN      
        1 maehtveasg gsaavsakvn fvtrnlqevl gearmneimk erdlkiywgt attgkphvay
       61 fvpmskiadf lkagcevtil fadlhayldn mkapwgllel rtqyyeqvik amlksigvpl
      121 eklkfvkgst yqlskefsmd vyrlvslvte hdakkagaev vkqvehplls smlypllqal
      181 deeylhvdaq fggvdqrkif tfaekylpql gyekrihfmn pmvpglsggk mssseedski
      241 dlldsptavk kklkkafcep gnledngvla fvkhvlvplq kgaelvivrd eahggnitys
      301 syeeleedfk nlklhpgdlk amvevhlnrl ldpiretfsd pklqklaada yppptkavfd
      361 evtpqrldlr vgrfievndh pdadtlyvsr vdlgepagpr tvvsglrnlv pkeallnqnf
      421 vflcnlkpsk mrgaesqamv lcasvqeprq veplkppsnc tpgdrvfveg yedasiqpdg
      481 qlnpkkkiwe klqvdmkvsa slvaewrgca flvngqpvtc tslkgapik
//
LOCUS       XP_077501755             189 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112813 [Amblyomma americanum].
ACCESSION   XP_077501755
VERSION     XP_077501755.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645629.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..189
                     /product="uncharacterized protein LOC144112813"
                     /calculated_mol_wt=21645
     CDS             1..189
                     /gene="LOC144112813"
                     /coded_by="XM_077645629.1:385..954"
                     /db_xref="GeneID:144112813"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedrrr rhlnrrltat mkrlsgvhvl
       61 nhehrfldas gepmlslfaa drgidqmski ivaalvkiyg pgiasasrar pgevyvvhrc
      121 rrcgakghkt dhcwaycspp rrcrsrlkcs ckrpflgpph cfladrerpv scpyslsvei
      181 darhqnrvv
//
LOCUS       XP_077501756            1022 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112814 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501756
VERSION     XP_077501756.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645630.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1022
                     /product="uncharacterized protein LOC144112814 isoform X1"
                     /calculated_mol_wt=115618
     Region          16..576
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1022
                     /gene="LOC144112814"
                     /coded_by="XM_077645630.1:86..3154"
                     /db_xref="GeneID:144112814"
ORIGIN      
        1 mvkvrkvilt ptrrvymppq lvcksrllqh cdpdyalriv vrdddcklis fslgackddf
       61 lsavikppls sglkiggrrf mflgcstsql rshglwfyah dnqgrtaeqi rdgigdlsti
      121 vsvpkfmarl gqafsqslgl ltvpsqntsv epdirhaapg rssdgshrey ifsdgigris
      181 hgllrkvhha leleegeepc avqiryggck gmllldptls gcrivfresm rkfhsdhsdl
      241 yvlktskprv lylnrpmiti leqsgikaev flmmqnkild sfidsmmdph eaahvlrayc
      301 alclpykela gvgidltvep ffralvravn kkvlkelrtk arilvppnyg rtmfgvldet
      361 gtleygqvfv qysndmlryk vndpatileg dvivtknpcm hpgdirklea vnvpqlhhvr
      421 dcivfpqkge rphpdemags dldgdeysvl wyedlifnnn cnpmhyysdp pkerkasigv
      481 qdmvdffcqy ikgdkiglia nahlawadil dsginshrcr elarkcavnl dfakcgdlkg
      541 lqnsekppmy pdfmekldtk ntycsrkvlg qlyrnckkve lsteclevve dslpdprlll
      601 egrekflvea tstykryakk irallksyri esesealsga vsnlskymke ndptdmamvl
      661 esqvehvvrr treeffseql deahenlkas awyqvtyelq sseggiqsfp wvvsdvlmri
      721 vvntssclpv palrnsfsqr lgalllgvaq pgggdqdgaq hgdtqvltnl lrlmydwids
      781 sreflfikda eelglyknim reacvkvsrs lsrdmpphkl vilclrfaca wclkifqggs
      841 dgeviskecr rryrlghlal itlnrlsmsg nlaylrrape gcpsteliri yinredeeff
      901 qillryedil tkimkdwcgv qdiqcdlktd smdewflqlm vtgsrwaler lkeivvypsf
      961 revlllafer eknaiagtlq scdrrklran arlcpnasgl pggswerkag sarraglmgr
     1021 aa
//
LOCUS       XP_077501757             980 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112814 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501757
VERSION     XP_077501757.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645631.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..980
                     /product="uncharacterized protein LOC144112814 isoform X2"
                     /calculated_mol_wt=111210
     Region          16..576
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..980
                     /gene="LOC144112814"
                     /coded_by="XM_077645631.1:86..3028"
                     /db_xref="GeneID:144112814"
ORIGIN      
        1 mvkvrkvilt ptrrvymppq lvcksrllqh cdpdyalriv vrdddcklis fslgackddf
       61 lsavikppls sglkiggrrf mflgcstsql rshglwfyah dnqgrtaeqi rdgigdlsti
      121 vsvpkfmarl gqafsqslgl ltvpsqntsv epdirhaapg rssdgshrey ifsdgigris
      181 hgllrkvhha leleegeepc avqiryggck gmllldptls gcrivfresm rkfhsdhsdl
      241 yvlktskprv lylnrpmiti leqsgikaev flmmqnkild sfidsmmdph eaahvlrayc
      301 alclpykela gvgidltvep ffralvravn kkvlkelrtk arilvppnyg rtmfgvldet
      361 gtleygqvfv qysndmlryk vndpatileg dvivtknpcm hpgdirklea vnvpqlhhvr
      421 dcivfpqkge rphpdemags dldgdeysvl wyedlifnnn cnpmhyysdp pkerkasigv
      481 qdmvdffcqy ikgdkiglia nahlawadil dsginshrcr elarkcavnl dfakcgdlkg
      541 lqnsekppmy pdfmekldtk ntycsrkvlg qlyrnckkve lsteclevve dslpdprlll
      601 egrekflvea tstykryakk irallksyri esesealsga vsnlskymke ndptdmamvl
      661 esqvehvvrr treeffseql deahenlkas awyqvtyelq sseggiqsfp wvvsdvlmri
      721 vvntssclpv palrnsfsqr lgalllgvaq pgggdqdgaq hgdtqvltnl lrlmydwids
      781 sreflfikda eelglyknim reacvkvsrs lsrdmpphkl vilclrfaca wclkifqggs
      841 dgeviskecr rryrlghlal itlnrlsmsg nlaylrrape gcpsteliri yinredeeff
      901 qillryedil tkimkdwcgv qdiqcdlktd smdewflqlm vtgsrwaler lkeivvypsf
      961 revlllafer eknaiagtlq
//
LOCUS       XP_077501758             918 aa            linear   INV 25-NOV-2025
DEFINITION  zinc metalloproteinase-disintegrin-like protein F1 [Amblyomma
            americanum].
ACCESSION   XP_077501758
VERSION     XP_077501758.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645632.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..918
                     /product="zinc metalloproteinase-disintegrin-like protein
                     F1"
                     /calculated_mol_wt=100921
     Region          <2..75
                     /region_name="Pep_M12B_propep"
                     /note="Reprolysin family propeptide; pfam01562"
                     /db_xref="CDD:460254"
     Region          123..320
                     /region_name="ZnMc_adamalysin_II_like"
                     /note="Zinc-dependent metalloprotease; adamalysin_II_like
                     subfamily. Adamalysin II is a snake venom zinc
                     endopeptidase. This subfamily contains other snake venom
                     metalloproteinases, as well as membrane-anchored
                     metalloproteases belonging to the ADAM family; cd04269"
                     /db_xref="CDD:239797"
     Site            order(259..260,263,269)
                     /site_type="active"
                     /db_xref="CDD:239797"
     Region          336..412
                     /region_name="DISIN"
                     /note="Homologues of snake disintegrins; smart00050"
                     /db_xref="CDD:214490"
     Region          414..558
                     /region_name="ACR"
                     /note="ADAM Cysteine-Rich Domain; smart00608"
                     /db_xref="CDD:214743"
     CDS             1..918
                     /gene="LOC144112817"
                     /coded_by="XM_077645632.1:154..2910"
                     /db_xref="GeneID:144112817"
ORIGIN      
        1 mkkdllplny fekhhenssh vidhpmkrtn khcyyhgtvr gqknswvavs tcnglsgvvf
       61 dgtelhyihp dlpehlffka sdmkaktwrc gfndsqdlwg nahkrakrsv viqppyksna
      121 rsryvelliv ndnkefikmk rnkeavfers keianivnal yaplnifial vgvivwtehd
      181 eivmssdgda tltkflqyrr erlarehpnd naqlitdivl dssvvgkalk gpictyeysg
      241 gvnkdhhevv gvvattvahe lghnfgmehd dkdtkchcpe krcimassts nppspsqwss
      301 cskqylqssf eqgmdfclwn lpedimgpvc gngfledgeq cdcgpmefck npccdaatck
      361 fkphaacaig accdvstcqv vkaatlcrdt vtecdlpeyc dgvsefcpvd vfvqdgtecg
      421 ggkaycfgkq crshddqcql lwgssgrmad qrcfernddg evnancgynr lnktykkcnk
      481 edvmcgtlhc vhlnerlefg iesaavqskf yidendkryt crsaivdlgl ftadvgqspn
      541 gakcgtnkac mnqkcvplek iygvkcpydc ynngvcnsrg nchcmvgyap pycnypgpgg
      601 ssdsgpasdp sagysfmvsm yiiflcivpl avitsfsiyy frrhlktwwm tkarkaairs
      661 raqqvgldrr ggrplskfni daeaikalni sapvtqpmvp qqplapsapp mppsqhqkph
      721 rsvsrergsf rnvdisgpvl qsssnshvap trpaparpap arpapgrptg vqrapscpsq
      781 gsmrgrprva rsasqrsngp rpahpppprp pappdgplyd dcatslefag tplafvhdep
      841 nsaqnrvvnr plparptpas sqptpvnsrp tpvnsrstvg gvaalaqkfe rnatessqph
      901 hgrvqptpgq rsytttls
//
LOCUS       XP_077501759             529 aa            linear   INV 25-NOV-2025
DEFINITION  tyrosine--tRNA ligase, cytoplasmic-like [Amblyomma americanum].
ACCESSION   XP_077501759
VERSION     XP_077501759.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645633.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..529
                     /product="tyrosine--tRNA ligase, cytoplasmic-like"
                     /calculated_mol_wt=58755
     Region          15..347
                     /region_name="nt_trans"
                     /note="nucleotidyl transferase superfamily; cl00015"
                     /db_xref="CDD:469580"
     Site            order(47,49..51,57,59..60,174,178,181,190,192..193,196,
                     222..223,230..233)
                     /site_type="active"
                     /db_xref="CDD:173902"
     Site            57..60
                     /site_type="other"
                     /note="HIGH motif"
                     /db_xref="CDD:173902"
     Site            order(84,87..88,137,139,141..143,145..146,148..151,
                     153..154,170,172..173,176..177)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:173902"
     Site            230..234
                     /site_type="other"
                     /note="KMSKS motif"
                     /db_xref="CDD:173902"
     Region          <342..529
                     /region_name="PLN02610"
                     /note="probable methionyl-tRNA synthetase"
                     /db_xref="CDD:215329"
     CDS             1..529
                     /gene="LOC144112818"
                     /coded_by="XM_077645633.1:115..1704"
                     /db_xref="GeneID:144112818"
ORIGIN      
        1 maehtveasg gsaavsakvn fvtrnlqevl gearmneimk erdlkiywgt attgkphvay
       61 fvpmskiadf lkagcevtil fadlhayldn mkapwgllel rtqyyeqvik amlksigvpl
      121 eklkfvkgst yqlskefsmd vyrlvslvte hdakkagaev vkqvehplls smlypllqal
      181 deeylhvdaq fggvdqrkif tfaekylpql gyekrihfmn pmvpglsggk mssseedski
      241 dlldsptavk kklkkafcep gnledngvla fvkhvlvplq kgaelvivrd eahggnitys
      301 syeeleedfk nlklhpgdlk amvevhlnrl ldpiretfsd pklqklaada yppptkavfd
      361 evtpqrldlr vgrfievndh pdadtlyvsr vdlgepagpr tvvsglrnlv pkeallnqnf
      421 vflcnlkpsk mrgaesqamv lcasvqeprq veplkppsnc tpgdrvfveg yedasiqpdg
      481 qlnpkkkiwe klqvdmkvsa slvaewrgca flvngqpvtc tslkgapik
//
LOCUS       XP_077501761             724 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112820 [Amblyomma americanum].
ACCESSION   XP_077501761
VERSION     XP_077501761.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645635.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..724
                     /product="uncharacterized protein LOC144112820"
                     /calculated_mol_wt=82661
     Region          <1..320
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..724
                     /gene="LOC144112820"
                     /coded_by="XM_077645635.1:48..2222"
                     /db_xref="GeneID:144112820"
ORIGIN      
        1 mitileqsgi kaevflmmqn kildsfidsm mdpheaahvl raycalclpy kelagvgidl
       61 tvepffralv ravnkkvlke lrtkarilvp pnygrtmfgv ldetgtleyg qvfvqysndm
      121 lrykvndpat ilegdvivtk npcmhpgdir kleavnvpql hhvrdcivfp qkgerphpde
      181 magsdldgde ysvlwyedli fnnncnpmhy ysdppkerka sigvqemvdf fcqyikgdki
      241 glianahlaw adildsgins hrcrelarkc avnldfakcg dlkglqnsek ppmypdfmek
      301 ldtkntycsr kvlgqlyrnc kkvelstecl evvedslpdp rlllegrekf lveatstykr
      361 yakkirallk syriesesea lsgavsnlsk ymkendptdm amvlesqveh vvrrtreeff
      421 seqldeahen lkasawyqvt yelqsseggi qsfpwvvsdv lmrivvntss clpvpalrns
      481 fsqrlgalll gvahpgggdq dgaqhgdtqv ltnllrlmyd widssreflf ikdaeelgly
      541 knimreacvk vsrflsrdmp phklvilclr facawclkif qggsdgevis kecrrryrlg
      601 hlalitlnrl smsgnlaylr rapegcpste liriyinred eeffqillry ediltkimkd
      661 wcgvqdiqcd lktdsmdewf lqlmvtgsrw alerlkeivv ypsfrevlll afereknaia
      721 gtlq
//
LOCUS       XP_077501762             780 aa            linear   INV 25-NOV-2025
DEFINITION  disintegrin and metalloproteinase domain-containing protein
            adm-2-like [Amblyomma americanum].
ACCESSION   XP_077501762
VERSION     XP_077501762.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645636.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..780
                     /product="disintegrin and metalloproteinase
                     domain-containing protein adm-2-like"
                     /calculated_mol_wt=84964
     Region          1..182
                     /region_name="ZnMc_adamalysin_II_like"
                     /note="Zinc-dependent metalloprotease; adamalysin_II_like
                     subfamily. Adamalysin II is a snake venom zinc
                     endopeptidase. This subfamily contains other snake venom
                     metalloproteinases, as well as membrane-anchored
                     metalloproteases belonging to the ADAM family; cd04269"
                     /db_xref="CDD:239797"
     Site            order(121..122,125,131)
                     /site_type="active"
                     /db_xref="CDD:239797"
     Region          198..274
                     /region_name="DISIN"
                     /note="Homologues of snake disintegrins; smart00050"
                     /db_xref="CDD:214490"
     Region          276..420
                     /region_name="ACR"
                     /note="ADAM Cysteine-Rich Domain; smart00608"
                     /db_xref="CDD:214743"
     CDS             1..780
                     /gene="LOC144112822"
                     /coded_by="XM_077645636.1:139..2481"
                     /db_xref="GeneID:144112822"
ORIGIN      
        1 mkrnkeavfe rskeianivn alyaplnifi alvgvivwte hdeivmssdg datltkflqy
       61 rrerlarehp ndnaqlitdi vldssvvgka lkgpictyey sggvnkdhhe vvgvvattva
      121 helghnfgme hddtdtkchc pekrcimass tsnppspsqw sscskqylqs sfeqgmdfcl
      181 wnlpedimgp vygngfleeg eqcdcgpmef cknpccdaat ckfkphaaca igaccdvstc
      241 qvvkaatlcr dtvtecdlpe ycdgvsefcp vdvfvqdgte cgggkaycfg kqcrshddqc
      301 qllwgssgrm adqrcfernd dgevnancgy nrlnktykkc nkedvmcgtl hcvhlnerle
      361 fgiesaavqs kfyidendkr ytcrsaivdl glftadvgqs pngakcgtnk acmnqkcvpl
      421 ekiygvkcpy dcynngvcns rgnchcmvgy appycnypgp ggssdsgpas dpsagysfmv
      481 smyiiflciv plavitsfsi yyfrrhlktw wmtkarkaai ksraqqvgld rrggrplskf
      541 nidaeaikal nisapvtqpm vpqqplapsa ppmppsqhqt phrsvsrerg sfrnvdisgp
      601 vlqsssnshv aptrpaparp aparpapgwp tgvqrapscp sqgsmrgrpr varsasqrsn
      661 gprpahpppp rppappdgpl yddcatslef agtplafvhd epnsaqnrvv nrplparptp
      721 assqptpvns rptpvnwrst vggvaalaqk fernatessq phhgrvqptp gqrsytttls
//
LOCUS       XP_077501763             529 aa            linear   INV 25-NOV-2025
DEFINITION  tyrosine--tRNA ligase, cytoplasmic-like [Amblyomma americanum].
ACCESSION   XP_077501763
VERSION     XP_077501763.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645637.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..529
                     /product="tyrosine--tRNA ligase, cytoplasmic-like"
                     /calculated_mol_wt=58795
     Region          15..347
                     /region_name="nt_trans"
                     /note="nucleotidyl transferase superfamily; cl00015"
                     /db_xref="CDD:469580"
     Site            order(47,49..51,57,59..60,174,178,181,190,192..193,196,
                     222..223,230..233)
                     /site_type="active"
                     /db_xref="CDD:173902"
     Site            57..60
                     /site_type="other"
                     /note="HIGH motif"
                     /db_xref="CDD:173902"
     Site            order(84,87..88,137,139,141..143,145..146,148..151,
                     153..154,170,172..173,176..177)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:173902"
     Site            230..234
                     /site_type="other"
                     /note="KMSKS motif"
                     /db_xref="CDD:173902"
     Region          <342..529
                     /region_name="PLN02610"
                     /note="probable methionyl-tRNA synthetase"
                     /db_xref="CDD:215329"
     CDS             1..529
                     /gene="LOC144112823"
                     /coded_by="XM_077645637.1:148..1737"
                     /db_xref="GeneID:144112823"
ORIGIN      
        1 maehtveang gsaavsakvn fvtrnlqevl gearmneimk erdlkiywgt attgkphvay
       61 fvpmskiadf lkagcevtil fadlhayldn mkapwgllel rtqyyeqvik amlksigvpl
      121 eklkfvkgst yqlskefsmd vyrlvslvte hdakkagaev vkqvqhplls smlypllqal
      181 deeylhvdaq fggvdqrkif tfaekylpql gyekrihfmn pmvpglsggk mssseedski
      241 dlldspaavk kklkkafcep gnledngvla fvkhvlvplq kgaelvierd eahggnitys
      301 syeeleedfk nlklhpgdlk amvevhlnrl ldpiretfsd pklqklaada yppptkavfd
      361 evtpqrldlr vgrfievndh pdadtlyvsr ldlgepagpr tvvsglrnlv pkeallnqnf
      421 vflcnlkpsk mrgaesqamv lcasvqeprq veplkppsnc tpgdrvfveg yedasiqpdg
      481 qlnpkkkiwe klqvdmkvsa slvaewrgca flvngqpvtc tslkgapik
//
LOCUS       XP_077501764             980 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112824 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501764
VERSION     XP_077501764.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645638.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..980
                     /product="uncharacterized protein LOC144112824 isoform X1"
                     /calculated_mol_wt=111171
     Region          16..576
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..980
                     /gene="LOC144112824"
                     /coded_by="XM_077645638.1:86..3028"
                     /db_xref="GeneID:144112824"
ORIGIN      
        1 mvkvrkvilt ptrrvymppq lvcksrllqn cdpdyalriv vrdddcklis fslgackddf
       61 lsavikppls sglkiggrrf mflgcstsql rshglwfyah dnqgrtaeqi rdgigdlsti
      121 vsvpkfmarl gqafsqslgl ltvpsqntsv epdirhaapg rssdgshrey ifsdgigris
      181 hgllrkvhha leleegeepc avqiryggck gmllldptls gccivfresm rkfhsdhsdl
      241 yvlktskprv lylnrpmiti leqsgikaev flmmqnkild sfidsmmdph eaahvlrayc
      301 alclpykela gvgidltvep ffralvravn kkvlkelrtk arilvppnyg rtmfgvldet
      361 gtleygqvfv qysndmlryk vndpatileg dvivtknpcm hpgdirklea vnvpqlhhvr
      421 dcivfpqkge rphpdemags dldgdeysvl wyedlifnnn cnpmhyysdp pkerkasigv
      481 qdmvdffcqy ikgdkiglia nahlvwadil dsginshrcr elarkcavnl dfakcgdlkg
      541 lqnsekppmy pdfmekldtk ntycsrkvlg qlyrnckkve lsteclevve dslpdprlll
      601 egrekflvea tstykryakk irallksyri esesealsga vsnlskymke ndptdmamvl
      661 esqvehvvrr treeffseql deahenlkas awyqvtyelq sseggiqsfp wvvsdvlmri
      721 vvntssclpv palrnsfsqr lgalllgvah pgggdqdgaq hgdtqvltnl lrlmydwids
      781 sreflfikda eelglyknim reacvkvsrs lsrdmpphkl vilclrfaca wclkifqggs
      841 dgeviskecr rryrlghlal itlnrlsmsg nlaylrrape gcpsteliri yinredeeff
      901 qillryedil tkimkdwcgv qdiqcdlktd smdewflqlm vtgsrwaler lkeivvypsf
      961 revlllafer eknaiagtlq
//
LOCUS       XP_077501765             810 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112824 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501765
VERSION     XP_077501765.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645639.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..810
                     /product="uncharacterized protein LOC144112824 isoform X2"
                     /calculated_mol_wt=92429
     Region          <17..406
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..810
                     /gene="LOC144112824"
                     /coded_by="XM_077645639.1:185..2617"
                     /db_xref="GeneID:144112824"
ORIGIN      
        1 mlfccvdhst panrmhvhha leleegeepc avqiryggck gmllldptls gccivfresm
       61 rkfhsdhsdl yvlktskprv lylnrpmiti leqsgikaev flmmqnkild sfidsmmdph
      121 eaahvlrayc alclpykela gvgidltvep ffralvravn kkvlkelrtk arilvppnyg
      181 rtmfgvldet gtleygqvfv qysndmlryk vndpatileg dvivtknpcm hpgdirklea
      241 vnvpqlhhvr dcivfpqkge rphpdemags dldgdeysvl wyedlifnnn cnpmhyysdp
      301 pkerkasigv qdmvdffcqy ikgdkiglia nahlvwadil dsginshrcr elarkcavnl
      361 dfakcgdlkg lqnsekppmy pdfmekldtk ntycsrkvlg qlyrnckkve lsteclevve
      421 dslpdprlll egrekflvea tstykryakk irallksyri esesealsga vsnlskymke
      481 ndptdmamvl esqvehvvrr treeffseql deahenlkas awyqvtyelq sseggiqsfp
      541 wvvsdvlmri vvntssclpv palrnsfsqr lgalllgvah pgggdqdgaq hgdtqvltnl
      601 lrlmydwids sreflfikda eelglyknim reacvkvsrs lsrdmpphkl vilclrfaca
      661 wclkifqggs dgeviskecr rryrlghlal itlnrlsmsg nlaylrrape gcpsteliri
      721 yinredeeff qillryedil tkimkdwcgv qdiqcdlktd smdewflqlm vtgsrwaler
      781 lkeivvypsf revlllafer eknaiagtlq
//
LOCUS       XP_077501766             917 aa            linear   INV 25-NOV-2025
DEFINITION  zinc metalloproteinase-disintegrin-like protein F1 [Amblyomma
            americanum].
ACCESSION   XP_077501766
VERSION     XP_077501766.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645640.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..917
                     /product="zinc metalloproteinase-disintegrin-like protein
                     F1"
                     /calculated_mol_wt=100752
     Region          <2..75
                     /region_name="Pep_M12B_propep"
                     /note="Reprolysin family propeptide; pfam01562"
                     /db_xref="CDD:460254"
     Region          122..319
                     /region_name="ZnMc_adamalysin_II_like"
                     /note="Zinc-dependent metalloprotease; adamalysin_II_like
                     subfamily. Adamalysin II is a snake venom zinc
                     endopeptidase. This subfamily contains other snake venom
                     metalloproteinases, as well as membrane-anchored
                     metalloproteases belonging to the ADAM family; cd04269"
                     /db_xref="CDD:239797"
     Site            order(258..259,262,268)
                     /site_type="active"
                     /db_xref="CDD:239797"
     Region          335..411
                     /region_name="DISIN"
                     /note="Homologues of snake disintegrins; smart00050"
                     /db_xref="CDD:214490"
     Region          413..557
                     /region_name="ACR"
                     /note="ADAM Cysteine-Rich Domain; smart00608"
                     /db_xref="CDD:214743"
     CDS             1..917
                     /gene="LOC144112828"
                     /coded_by="XM_077645640.1:67..2820"
                     /db_xref="GeneID:144112828"
ORIGIN      
        1 mkkdllplny fekhhenssh vidhpmkrtn khcyyhgtvr gqknswvavs tcnglsgvvf
       61 dgteihyihp dlpehlffka sdmkaktwrc gfndsqdlwg nahkrgrsvv iqppykcnar
      121 sryvellivn dnkefikmkr nkeavfersk eianivnaly aplnifialv gvivwtehde
      181 ivmssdgdat ltkflqyrre rlarehpndn aqlitdivld ssvvgkalkg pictyeysgg
      241 vnkdhhevvg vvattvahel ghnfgmehdd kdtkchcpek rcimasstsn ppspsqwssc
      301 skqylqssfe qgmdfclwnl pedimgpvcg ngfleegeqc dcgpmefckt pccdaatckf
      361 kphaacaiga ccdvstcqvv kaatlcrdtv tecdlpeycd gvsefcpvdv fvqdgtecgg
      421 gkaycfgkqc rshddqcqll wgssgrmadq rcfkrnddge vnancgynrl nktykkcnke
      481 dvmcgtlhcv hlnerldfgi esaavqskfy idendkrytc rsaivdlglf tadvgqspng
      541 ancgnnkacm nqkcvpleki ygvkcpydcy nngvcnsrgn chcmvgyapp ycnypgpggs
      601 sdsgpasdps agysfmvsmy iiflcivpla vitsfsiyyf rrhlktwwmt karkaaiksr
      661 aqqvgldrrg grplskfnid aeaikalnis apvtqpmvpq qplapsappm ppsqhqkphr
      721 svsrergsfr nvdisgpvlq sssnshvapt rpaparpapa rpapgrptgv qrapscpsqg
      781 smrgrprvar sasqrsngpr pahpppprpp appdgplydd catslefagt plafvhdepn
      841 saqnrvvnrp lparptpass qptpvnsrpt pvnsrstvgg vaalaqkfer natessqphh
      901 grvqptpgqr sytttls
//
LOCUS       XP_077501767             529 aa            linear   INV 25-NOV-2025
DEFINITION  tyrosine--tRNA ligase, cytoplasmic-like [Amblyomma americanum].
ACCESSION   XP_077501767
VERSION     XP_077501767.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645641.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..529
                     /product="tyrosine--tRNA ligase, cytoplasmic-like"
                     /calculated_mol_wt=58808
     Region          15..347
                     /region_name="nt_trans"
                     /note="nucleotidyl transferase superfamily; cl00015"
                     /db_xref="CDD:469580"
     Site            order(47,49..51,57,59..60,174,178,181,190,192..193,196,
                     222..223,230..233)
                     /site_type="active"
                     /db_xref="CDD:173902"
     Site            57..60
                     /site_type="other"
                     /note="HIGH motif"
                     /db_xref="CDD:173902"
     Site            order(84,87..88,137,139,141..143,145..146,148..151,
                     153..154,170,172..173,176..177)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:173902"
     Site            230..234
                     /site_type="other"
                     /note="KMSKS motif"
                     /db_xref="CDD:173902"
     Region          <342..529
                     /region_name="PLN02610"
                     /note="probable methionyl-tRNA synthetase"
                     /db_xref="CDD:215329"
     CDS             1..529
                     /gene="LOC144112829"
                     /coded_by="XM_077645641.1:137..1726"
                     /db_xref="GeneID:144112829"
ORIGIN      
        1 maehtveang gsaavsakvn fvtrnlqevl gearmneimk erdlkiywgt attgkphvay
       61 fvpmskiadf lkagcevtil fadlhayldn mkapwgllel rtqyyeqvik amlksigvpl
      121 eklkfvkgst yqlskefsmd vyrlvslvte hdakkagaev vkqvehplls smlypllqal
      181 deeylhvdaq fggvdqrkif tfaekylpql gyekrihfmn pmvpglsgvk mssseedski
      241 dllespaavk kklkkafcvp gnledngvla fvkhvlvplq kgaelvierd eahggnitys
      301 syeeleedfk nlklhpgdlk amvevhlnrl ldpiretfsd pklqklaada yppptkavfd
      361 evtpqrldlr vgrfievndh pdadtlyvsr vdlgepagpr tvvsglrnlv pkeallnqnf
      421 vflcnlkpsk mrgaesqamv lcasvqeprq veplkppsnc tpgdrvfveg yedasiqpdg
      481 qlnpkkkiwe klqvdmkvsa slvaewrgca flvngqpvtc tslkgapik
//
LOCUS       XP_077501769             918 aa            linear   INV 25-NOV-2025
DEFINITION  zinc metalloproteinase-disintegrin-like protein F1 [Amblyomma
            americanum].
ACCESSION   XP_077501769
VERSION     XP_077501769.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645643.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..918
                     /product="zinc metalloproteinase-disintegrin-like protein
                     F1"
                     /calculated_mol_wt=100877
     Region          <2..75
                     /region_name="Pep_M12B_propep"
                     /note="Reprolysin family propeptide; pfam01562"
                     /db_xref="CDD:460254"
     Region          123..320
                     /region_name="ZnMc_adamalysin_II_like"
                     /note="Zinc-dependent metalloprotease; adamalysin_II_like
                     subfamily. Adamalysin II is a snake venom zinc
                     endopeptidase. This subfamily contains other snake venom
                     metalloproteinases, as well as membrane-anchored
                     metalloproteases belonging to the ADAM family; cd04269"
                     /db_xref="CDD:239797"
     Site            order(259..260,263,269)
                     /site_type="active"
                     /db_xref="CDD:239797"
     Region          336..412
                     /region_name="DISIN"
                     /note="Homologues of snake disintegrins; smart00050"
                     /db_xref="CDD:214490"
     Region          414..558
                     /region_name="ACR"
                     /note="ADAM Cysteine-Rich Domain; smart00608"
                     /db_xref="CDD:214743"
     CDS             1..918
                     /gene="LOC144112832"
                     /coded_by="XM_077645643.1:159..2915"
                     /db_xref="GeneID:144112832"
ORIGIN      
        1 mkkdllplny fekhhenssh vidhpmkrtn khcyyhgtvr gqknswvavs tcnglsgvvf
       61 dgtelhyihp dlpehlffka sdmkaktwrc gfndsqdlwg nahkrakrsv viqppyksna
      121 rsryvelliv ndnkefikmk rnkeavfers keianivnal yaplnifial vgvivwtehd
      181 eivmssdgda tltkflqyrr erlarehpnd naqlitdivl dssvvgkalk gpictyeysg
      241 gvnkdhhevv gvvattvahe lghnfgmehd dkdtkchcpe krcimassts nppspsqwss
      301 cskqylqssf eqgmdfclwn lpedimgpvc gngfleegeq cdcgpmefck npccdaatck
      361 fkphaacaig accdvstcqv vkaatlcrdt vtecdlpeyc dgvsefcpvd vfvqdgtecg
      421 ggkaycfgkq crshddqcql lwgssgrmad qrcfernddg evnancgynr lnktykkcnk
      481 edvmcgtlhc vhlnerlefg iesaavqskf yidendkryt crsaivdlgl ftadvgqspn
      541 gakcgtnkac mnqkcvplek iygvkcpydc ynngvcnsrg nchcmvgyap pycnypgpgg
      601 ssdsgpasdp sagysfmvsm yiiflcivpl avitsfsiyy frrhlktwwm tkarkaaiks
      661 raqqvgldrr ggrplskfni daeaikalni sapvtqpmvp qqplapsapp mppsqhqkph
      721 rsvsrergsf rnvdisgpvl qsssnshvap trpaparpap arpapgrpag vqrapscpsq
      781 gsmrgrprva rsasqrsngp rpahpppprp pappdgplyd dcatslefag tplafvhdep
      841 nsaqnrvvnr plparptpas sqptpvnsrp tpvnsrstvg gvaalaqkfe rnatessqph
      901 hgrvqptpgq rsytttls
//
LOCUS       XP_077501770             529 aa            linear   INV 25-NOV-2025
DEFINITION  tyrosine--tRNA ligase, cytoplasmic-like [Amblyomma americanum].
ACCESSION   XP_077501770
VERSION     XP_077501770.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645644.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..529
                     /product="tyrosine--tRNA ligase, cytoplasmic-like"
                     /calculated_mol_wt=58785
     Region          15..347
                     /region_name="nt_trans"
                     /note="nucleotidyl transferase superfamily; cl00015"
                     /db_xref="CDD:469580"
     Site            order(47,49..51,57,59..60,174,178,181,190,192..193,196,
                     222..223,230..233)
                     /site_type="active"
                     /db_xref="CDD:173902"
     Site            57..60
                     /site_type="other"
                     /note="HIGH motif"
                     /db_xref="CDD:173902"
     Site            order(84,87..88,137,139,141..143,145..146,148..151,
                     153..154,170,172..173,176..177)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:173902"
     Site            230..234
                     /site_type="other"
                     /note="KMSKS motif"
                     /db_xref="CDD:173902"
     Region          <342..529
                     /region_name="PLN02610"
                     /note="probable methionyl-tRNA synthetase"
                     /db_xref="CDD:215329"
     CDS             1..529
                     /gene="LOC144112833"
                     /coded_by="XM_077645644.1:111..1700"
                     /db_xref="GeneID:144112833"
ORIGIN      
        1 maehtveasg gsaavsakvn fvtrnlqevl gearmneimk erdlkiywgt attgkphvay
       61 fvpmskiadf lkagcevtil fadlhayldn mkapwgllel rtqyyeqvik amlksigvpl
      121 eklkfvkgst yqlskefsmd vyrlvslvte hdakkagaev vkqvehplls smlypllqal
      181 deeylhvdaq fggvdqrkif tfaekylpql gyekrihfmn pmvpglsggk mssseedski
      241 dlldsptavk kklkkafcep gnledngvla fvkhvlvplq kgaelvierd eahggnitys
      301 syeeleedfk nlklhpgdlk amvevhlnrl ldpiretfsd pklqklaada yppptkavfd
      361 evtpqrldlr vgrfievndh pdadtlyvsr vdlgepagpr tvvsglrnlv pkeallnqnf
      421 vflcnlkpsk mrgaesqamv lcasvqeprq veplkppsnc tpgdrvfveg yedasiqpdg
      481 qlnpkkkiwe klqvdmkvsa slvaewrgca flvngqpvtc tslkgapik
//
LOCUS       XP_077501771            1287 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112834 [Amblyomma americanum].
ACCESSION   XP_077501771
VERSION     XP_077501771.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645645.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1287
                     /product="uncharacterized protein LOC144112834"
                     /calculated_mol_wt=146074
     Region          323..883
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1287
                     /gene="LOC144112834"
                     /coded_by="XM_077645645.1:273..4136"
                     /db_xref="GeneID:144112834"
ORIGIN      
        1 msvkrgkkgr avkstspsdg tsssaktscp nseltssegf kvtqcnahfe hcsnglylyf
       61 vdvrkrsstr frllvrysti friilrktsp tstivylqll hppllykvca gnsatsqcnr
      121 egdylpqfdl sdttpwdrav efgegpfkcg advlgrsrmm rltlsgprhw aplcnlilrt
      181 rknaqvfyap vrelqardlk avsrpsvqdf gvvyallsvw sesfqvtdel amdaqscret
      241 llelsaraee cpraleealf eiyfslsqgk vfwfrsaleh lyfryrahrq ridvftgsvi
      301 aqelpshmvk vrkviltptr rvymppqlvc ksrllqhcdp dyalrivvrd ddcklisfsl
      361 gackddflsa vikpplssgl kiggrrfmfl gcstsqlrsh glwfyahdnq grtaeqirdg
      421 igdlstivsv pkfmarlgqa fsqslglltv psqntsvepd irhaapgrss dgshreyifs
      481 dgigrishgl lrkvhhalel eegeepcavq iryggckgml lldptlsgcr ivfresmrkf
      541 hsdhsdlyvl ktskprvlyl nrpmitileq sgikaevflm mqnkildsfi dsmmdpheaa
      601 hvlraycalc lpykelagvg idltvepffr alvravnkkv lkelrtkari lvppnygrtm
      661 fgvldetgtf eygqvfvqys ndmlrykvnd patilegdvi vtknpcmhpg dirkleavnv
      721 pqlhhvrdci vfpqkgerph pdemagsdld gdeysvlwye dlifnnncnp mhyysdppke
      781 rkasigvqem vdffcqyikg dkiglianah lawadildsg inshrcrela rkcavnldfa
      841 kcgdlkglqn sekppmypdf mekldtknty csrkvlgqly rnckkvelst eclevvedsl
      901 pdprlllegr ekflveatst ykryakkira llkshriese sealsgavsn lskymkendp
      961 tdmamvlesq vehvvrrtre effseqldea henlkasawy qvtyelqsse ggiqsfpwvv
     1021 sdvlmrivvn tssclpvpal rnsfsqrlga lllgvahpgg gdqdgaqhgd tqvltnllrl
     1081 mydwidssre flfikdaeel glyknimrea cvkvsrslsr dmpphklvil clrfacawcl
     1141 kifqggsdge viskecrrry rlghlalitl nrlsmsgnla ylrrapegcp cteliriyin
     1201 redeeffqil lryediltki mkdwcgvqdi qcdlktdsmd ewflqlmvtg srwalerlke
     1261 ivvypsfrev lllaferekn aiagtlq
//
LOCUS       XP_077501772             309 aa            linear   INV 25-NOV-2025
DEFINITION  inactive selenide, water dikinase-like protein isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077501772
VERSION     XP_077501772.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645646.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..309
                     /product="inactive selenide, water dikinase-like protein
                     isoform X1"
                     /calculated_mol_wt=33779
     Region          1..278
                     /region_name="PurM-like"
                     /note="AIR (aminoimidazole ribonucleotide) synthase
                     related protein. This family includes Hydrogen
                     expression/formation protein HypE, AIR synthases, FGAM
                     (formylglycinamidine ribonucleotide) synthase and
                     Selenophosphate synthetase (SelD). The N-terminal
                     domain...; cl10019"
                     /db_xref="CDD:471963"
     Site            order(14..17,19,43,54,57,60,96,103..104,108)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:100004"
     Site            order(39,43,94..96)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:100004"
     CDS             1..309
                     /gene="LOC144112835"
                     /coded_by="XM_077645646.1:343..1272"
                     /db_xref="GeneID:144112835"
ORIGIN      
        1 mdssvvplkd sgyslvqtid yfypsvedty lagkivcanv vsdlyaigvt rcdnvlmvma
       61 astkltqkqr divvpllirg vkdlaeqagt mvtggqtmln pwpmiggvas svcreseiis
      121 galrcgslea lnatrlfsfe gekrpsdkrp dnaqpgdvlv ltkplgtqva mnahqwlekp
      181 erhaqldkag isedvrrvyl raidfmarln lngarlmhkh gchgatdvtg fgllghtrtw
      241 ckcssaqwgg llmclpkena easckeieqq egypawiied vvegervarl tdslrvlevp
      301 sadkeselw
//
LOCUS       XP_077501773             309 aa            linear   INV 25-NOV-2025
DEFINITION  inactive selenide, water dikinase-like protein isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077501773
VERSION     XP_077501773.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645647.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..309
                     /product="inactive selenide, water dikinase-like protein
                     isoform X1"
                     /calculated_mol_wt=33779
     Region          1..278
                     /region_name="PurM-like"
                     /note="AIR (aminoimidazole ribonucleotide) synthase
                     related protein. This family includes Hydrogen
                     expression/formation protein HypE, AIR synthases, FGAM
                     (formylglycinamidine ribonucleotide) synthase and
                     Selenophosphate synthetase (SelD). The N-terminal
                     domain...; cl10019"
                     /db_xref="CDD:471963"
     Site            order(14..17,19,43,54,57,60,96,103..104,108)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:100004"
     Site            order(39,43,94..96)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:100004"
     CDS             1..309
                     /gene="LOC144112835"
                     /coded_by="XM_077645647.1:323..1252"
                     /db_xref="GeneID:144112835"
ORIGIN      
        1 mdssvvplkd sgyslvqtid yfypsvedty lagkivcanv vsdlyaigvt rcdnvlmvma
       61 astkltqkqr divvpllirg vkdlaeqagt mvtggqtmln pwpmiggvas svcreseiis
      121 galrcgslea lnatrlfsfe gekrpsdkrp dnaqpgdvlv ltkplgtqva mnahqwlekp
      181 erhaqldkag isedvrrvyl raidfmarln lngarlmhkh gchgatdvtg fgllghtrtw
      241 ckcssaqwgg llmclpkena easckeieqq egypawiied vvegervarl tdslrvlevp
      301 sadkeselw
//
LOCUS       XP_077501774             253 aa            linear   INV 25-NOV-2025
DEFINITION  inactive selenide, water dikinase-like protein isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077501774
VERSION     XP_077501774.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645648.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..253
                     /product="inactive selenide, water dikinase-like protein
                     isoform X2"
                     /calculated_mol_wt=27688
     Region          <11..222
                     /region_name="PurM-like"
                     /note="AIR (aminoimidazole ribonucleotide) synthase
                     related protein. This family includes Hydrogen
                     expression/formation protein HypE, AIR synthases, FGAM
                     (formylglycinamidine ribonucleotide) synthase and
                     Selenophosphate synthetase (SelD). The N-terminal
                     domain...; cl10019"
                     /db_xref="CDD:471963"
     CDS             1..253
                     /gene="LOC144112835"
                     /coded_by="XM_077645648.1:710..1471"
                     /db_xref="GeneID:144112835"
ORIGIN      
        1 mvmaastklt qkqrdivvpl lirgvkdlae qagtmvtggq tmlnpwpmig gvassvcres
       61 eiisgalrcg slealnatrl fsfegekrps dkrpdnaqpg dvlvltkplg tqvamnahqw
      121 lekperhaql dkagisedvr rvylraidfm arlnlngarl mhkhgchgat dvtgfgllgh
      181 trtwckcssa qwggllmclp kenaeascke ieqqegypaw iiedvveger varltdslrv
      241 levpsadkes elw
//
LOCUS       XP_077501775             852 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112836 [Amblyomma americanum].
ACCESSION   XP_077501775
VERSION     XP_077501775.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645649.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..852
                     /product="uncharacterized protein LOC144112836"
                     /calculated_mol_wt=95699
     Region          518..>836
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..852
                     /gene="LOC144112836"
                     /coded_by="XM_077645649.1:1466..4024"
                     /db_xref="GeneID:144112836"
ORIGIN      
        1 meppskpllp gpmpptsafc ardrqdncre malrvhclds gwgtptasql rrllglqcav
       61 gqvvagdcge vevyespnwt anvtlklers pkdsfmhivl kwcdqyqngs nngaaagksg
      121 ppewwlasee leefcdpyst qiatehkdvp vnafalgcfl gyntfvahfd stytmpppps
      181 plssaksscs hsgksmsvkr gkkgrgakss cpsdgtsssa ktacpnselt spegfkvtqc
      241 nahfehcfng lylyfvdvrk rsstrfrllv rysticriil rktsptctiv ylqllhppll
      301 ykvcagnsat sqcnregdyl pqfdlsdttp wdravefgeg pfkcgadvlg rsrvmrltls
      361 gprhwvplcn lilrtrknvq vfyapvrelq arelkavarp svqdfgvmya llsvwsesfq
      421 vtdelamdaq scretllela ahaeecpral eealfeiyfs lsqgkvfwfr salehlyfry
      481 rahrqridvf tgsviaqelp shmvkvrkvi ltptrrvymp pqlvcksrll qhcdpdyalr
      541 ivvrdddckl isfslgackd dflsavikpp lssglkiggr rfmflgcsts qlrnhgiwfy
      601 ahdnqgrtae qirdgigdls tipsvpkfma rlgqafsqsl glltvprqnt sveqdirhaa
      661 pgrssdgshr eyifsdgigr ishgllrkvh haleleegee pcaiqirygg ckgmllldpt
      721 lsgcrivfre smrkfhsdhs dlyvlktskp rvlylnrpmi tileqsgika evflmlqnki
      781 ldsfidsmmd pheaahvrnf lmfaafvpcm vsvfalfyss rshtrritep yvrqlilfnr
      841 ntethvrhpi pf
//
LOCUS       XP_077501776             628 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112837 [Amblyomma americanum].
ACCESSION   XP_077501776
VERSION     XP_077501776.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645650.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..628
                     /product="uncharacterized protein LOC144112837"
                     /calculated_mol_wt=71905
     Region          <1..224
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..628
                     /gene="LOC144112837"
                     /coded_by="XM_077645650.1:161..2047"
                     /db_xref="GeneID:144112837"
ORIGIN      
        1 mfgvldetgt leygqvfvqy sndmlrykvn dpatilegdv ivtknpcmhp gdirkleavn
       61 vpqlhhvrdc ivfpqkgerp hpdemagsdl dgdeysvlwy edlifnnncn pmhyysdppk
      121 erkasigvqd mvdffcqyik gdkigliana hlvwadildg ginshrcrel arkcavnldf
      181 akcgdlkglq nsekppmypd fmekldtknt ycsrkvlgql yrnckkvels teclevvees
      241 lpdprllleg reqfmkeats aykryakkiw allksyries esealsgavs klskymkend
      301 stdmamvles qvehvvrqtr eeffseqlde ahenlkasaw yqvtyelqss eggiqsfpwv
      361 vsdvlmrivv ntssclpvpa srnsfsqrlg alllrvphpg ggdqddaqhg dtqvltnllr
      421 lmydwidssr eflfikdaee lglyknimre acvkvtrsls rdmpphklvi lclrfacawc
      481 lkifqggsdg eviskecrrr yrlghlalit lnrlsmsgnl aylcrapegc psteliriyi
      541 nredevlfei lrryedivte imtdwsgved iqcdlkmdrm ddwflqlmvt gsrwalerlk
      601 eivvypsfre vlllaferkk naiagtle
//
LOCUS       XP_077501777             281 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112838 [Amblyomma americanum].
ACCESSION   XP_077501777
VERSION     XP_077501777.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645651.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..281
                     /product="uncharacterized protein LOC144112838"
                     /calculated_mol_wt=32169
     Region          13..100
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..281
                     /gene="LOC144112838"
                     /coded_by="XM_077645651.1:55..900"
                     /db_xref="GeneID:144112838"
ORIGIN      
        1 maaavgprav straswtdre vecflalise kkvsealdsr rqrnqdvfkd lqaemanlgy
       61 hwtwqqlrnc wknlkkrfta erleqersga apsawkwydh msallshrpm vqareygvds
      121 qdvppdygdn sqldisdadt rteeddadil qeqsepstss rsppqkrrrm geaklekvll
      181 nvqrkneefw tkqseiqfqq qkqlladenk hmqellqgvs ttflqgtqtf mgqffsqqaa
      241 lvaslqhmps fpqfmgppnm nlqhqfpppp patntpptpk h
//
LOCUS       XP_077501778             281 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112839 [Amblyomma americanum].
ACCESSION   XP_077501778
VERSION     XP_077501778.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645652.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..281
                     /product="uncharacterized protein LOC144112839"
                     /calculated_mol_wt=32169
     Region          13..100
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..281
                     /gene="LOC144112839"
                     /coded_by="XM_077645652.1:246..1091"
                     /db_xref="GeneID:144112839"
ORIGIN      
        1 maaavgprav straswtdre vecflalise kkvsealdsr rqrnqdvfkd lqaemanlgy
       61 hwtwqqlrnc wknlkkrfta erleqersga apsawkwydh msallshrpm vqareygvds
      121 qdvppdygdn sqldisdadt rteeddadil qeqsepstss rsppqkrrrm geaklekvll
      181 nvqrkneefw tkqseiqfqq qkqlladenk hmqellqgvs ttflqgtqtf mgqffsqqaa
      241 lvaslqhmps fpqfmgppnm nlqhqfpppp patntpptpk h
//
LOCUS       XP_077501779             976 aa            linear   INV 25-NOV-2025
DEFINITION  eukaryotic translation initiation factor 2-alpha kinase 3-like
            [Amblyomma americanum].
ACCESSION   XP_077501779
VERSION     XP_077501779.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645653.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..976
                     /product="eukaryotic translation initiation factor 2-alpha
                     kinase 3-like"
                     /calculated_mol_wt=108246
     Region          48..333
                     /region_name="Luminal_IRE1_like"
                     /note="The Luminal domain, a dimerization domain, of
                     Inositol-requiring protein 1-like proteins; cl14874"
                     /db_xref="CDD:276097"
     Site            order(128..132,137,139..142,144..146,158,160..162,
                     182..183,191,193,208,210..212)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:188874"
     Region          525..944
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(538..541,544,546,559,561,591,753..756,800,804..805,
                     807,817..818)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..976
                     /gene="LOC144112840"
                     /coded_by="XM_077645653.1:137..3067"
                     /db_xref="GeneID:144112840"
ORIGIN      
        1 mhlfhvflil lysfvaqgtd essakelpdc skapkpsdvd egktfrelfi vstldgkita
       61 ldpkahgnlv wshetgpggl lssslsklem hnrgqrvrlv psldgllykf ngetveplpl
      121 sadfllrssy rlgddlvitg gkeartygin ihtgqvryic tmggcdnvke edfstedvlv
      181 vkrntqtvra ieprsgrekw nfsvgenqlt vlkglmpsca gsgghgsdad syvmeepanc
      241 sneegcpeks liqvvvpegl vastsgtkgs mewsvklpsp vvgawhlrng elevvdlfeh
      301 trilgldgpn rskqsnpvly lgmhdrqlyv qhsgsvetev sgassqlidp hrshlphvew
      361 kpylasapsr tpiintgnka apsvleesss srgalmikdd vldypfdtgy ylyrgvdldn
      421 dsvllcekee aaetfnehiv kslrfwwkei slivlatfli nifitkhvlq rfklkhlqel
      481 sskdaksssg sesatiappe qpvghpigqp svevcsavgd lpkytsryla dfepirclgk
      541 ggfglvlesr nklddctyav kriclpnnpe ardkvmreak alakldhssi vryynawles
      601 pppgwqehqd dvwrgsfdgc tscslsdkta tsaakdsswg tatrpfnplr pfedrfsfgd
      661 agnngafsss inssssgiti drrtsdghsl rlpfdayatt deedsliefe ksaksaeksk
      721 tksssgpken gerpkslsll esppvkvyly iqmqlckkes lkewlhdhst frnyesvldi
      781 fnqivsavey vhdnglihrd lkpsniffsm ddaikvgdfg lvtaltggsv vstplkapse
      841 difssksgrl tdqvgtqlym speqidglky nqsvdifslg liffellwpf stqmeriqvl
      901 lnakklnfps qfvkqykaec klieqllshd sekrpsardi rqhalfrafq psdeiahrar
      961 lrrrtlshrq ssslse
//
LOCUS       XP_077501780             664 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112841 [Amblyomma americanum].
ACCESSION   XP_077501780
VERSION     XP_077501780.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645654.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..664
                     /product="uncharacterized protein LOC144112841"
                     /calculated_mol_wt=71737
     Region          6..97
                     /region_name="FHA"
                     /note="forkhead associated (FHA) domain superfamily;
                     cl00062"
                     /db_xref="CDD:469597"
     Region          502..>635
                     /region_name="PRK13335"
                     /note="superantigen-like protein SSL3; Reviewed"
                     /db_xref="CDD:139494"
     CDS             1..664
                     /gene="LOC144112841"
                     /coded_by="XM_077645654.1:53..2047"
                     /db_xref="GeneID:144112841"
ORIGIN      
        1 meyypkivtl rrngtgfsvl plitgrcsvg gaagcdirip rsgaycclfl vgkneqarii
       61 nctgtphlkl ngkdvhdysv lshgdiveis yrkfrfdyge krkkensskv aaashqpkaa
      121 gdatptsnnk skkasteqag avaceegdde eegvplqsar disqscqesl ehmsvvaesp
      181 kvtvskqtfq yssqileisr esapekatlp trsgrssqts snegklkvqe kekeatvgsn
      241 qkpsskpska krtpaskrat aadvttelek agmsasknla fdntdvtaaa fgratraarp
      301 kyvetdkrtd vkmprqeakr tvsplktlea eqrpasnkel getpkqeaad tkkssrkilq
      361 aktarstrkv negeevpelg tagvspskcs satnvhvlde parptrgcrt rgsrkdivea
      421 pkekkvdqpq stkatraeht qgsrkvckeg etlqdvtgss lvmasttqrk dvtfdkhlqg
      481 easptkevsv trntrakrkv dlkeesiaek httaedligt vaeksgghqq rvasgeyapq
      541 endvqcsnsa kqvinpkitg dkevvddapa spnvevatve pasaggkala ttmepkrskr
      601 tmaavkvdva qtaneatprk trgrraealk talewaddkp gasrrtakdr seeepppkrt
      661 rkmv
//
LOCUS       XP_077501781             655 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin thioesterase trabid [Amblyomma americanum].
ACCESSION   XP_077501781
VERSION     XP_077501781.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645655.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..655
                     /product="ubiquitin thioesterase trabid"
                     /calculated_mol_wt=73214
     Region          7..30
                     /region_name="ZnF_RBZ"
                     /note="Zinc finger domain; smart00547"
                     /db_xref="CDD:197784"
     Region          8..27
                     /region_name="RanBP2-type Zn finger"
                     /note="RanBP2-type Zn finger [structural motif]"
                     /db_xref="CDD:275376"
     Region          63..86
                     /region_name="ZnF_RBZ"
                     /note="Zinc finger domain; smart00547"
                     /db_xref="CDD:197784"
     Region          64..83
                     /region_name="RanBP2-type Zn finger"
                     /note="RanBP2-type Zn finger [structural motif]"
                     /db_xref="CDD:275376"
     Region          120..143
                     /region_name="ZnF_RBZ"
                     /note="Zinc finger domain; smart00547"
                     /db_xref="CDD:197784"
     Region          122..141
                     /region_name="RanBP2-type Zn finger"
                     /note="RanBP2-type Zn finger [structural motif]"
                     /db_xref="CDD:275376"
     Region          202..283
                     /region_name="AnkUBD"
                     /note="Ankyrin ubiquitin-binding domain; pfam18418"
                     /db_xref="CDD:408216"
     Region          357..541
                     /region_name="OTU_ZRANB1"
                     /note="OTU (ovarian tumor) domain of Ubiquitin
                     thioesterase ZRANB1 and similar proteins; cd22767"
                     /db_xref="CDD:438604"
     Site            order(394..395,456,463,470..472,494,534,536)
                     /site_type="other"
                     /note="putative polypeptide substrate binding site
                     [polypeptide binding]"
                     /db_xref="CDD:438604"
     CDS             1..655
                     /gene="trbd"
                     /coded_by="XM_077645655.1:214..2181"
                     /db_xref="GeneID:144112843"
ORIGIN      
        1 msrqgckwtc qhctyenwpk alrcvicqnp rptlieeeas seerdiykis plvspdltss
       61 yqkwacgrct ymnwprsskc tqclhprgsp cnaqasppss qpqqattste vehtsadlrs
      121 kwtcgactye nwpksrkcim cnatrskgds pvgnqtpdpd dlrntptgvp avctggsnrl
      181 tgtteievqm saeenppsnn ylcslrrqar emdrlwlnac ravvdgepep isrylnsggd
      241 parqlthaea gllartanfd vgftlahmai rqerddilal llsnsgmthp vakrvpsdvg
      301 rdaaadirrh iaasfkqrkg pfscyfvtef vtfalpteik efhssvqkvl fdelldqdvq
      361 keleedsaii nwsleltetl gsrlyalwnr sagnclldsa lqatwgvfdr dnslrqvlgd
      421 slseaasvly prwreaealq arllhfslde aqwqedwatl vsmaaqpgsa leqmhvftla
      481 hvlrrpivvy gvkyvksfrg ealgyarfeg iyipllweps fcwkspialg ytrghfsalv
      541 pmepepevig gaeanprgdl qvsflplmta drqllplhfl tqgevgreee iikqwldccv
      601 teggllvaqq kvprsphlvl qmvdqwleay rklsphhrlt dsqsgyssdg dsdqe
//
LOCUS       XP_077501782             655 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin thioesterase trabid [Amblyomma americanum].
ACCESSION   XP_077501782
VERSION     XP_077501782.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645656.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..655
                     /product="ubiquitin thioesterase trabid"
                     /calculated_mol_wt=73214
     Region          7..30
                     /region_name="ZnF_RBZ"
                     /note="Zinc finger domain; smart00547"
                     /db_xref="CDD:197784"
     Region          8..27
                     /region_name="RanBP2-type Zn finger"
                     /note="RanBP2-type Zn finger [structural motif]"
                     /db_xref="CDD:275376"
     Region          63..86
                     /region_name="ZnF_RBZ"
                     /note="Zinc finger domain; smart00547"
                     /db_xref="CDD:197784"
     Region          64..83
                     /region_name="RanBP2-type Zn finger"
                     /note="RanBP2-type Zn finger [structural motif]"
                     /db_xref="CDD:275376"
     Region          120..143
                     /region_name="ZnF_RBZ"
                     /note="Zinc finger domain; smart00547"
                     /db_xref="CDD:197784"
     Region          122..141
                     /region_name="RanBP2-type Zn finger"
                     /note="RanBP2-type Zn finger [structural motif]"
                     /db_xref="CDD:275376"
     Region          202..283
                     /region_name="AnkUBD"
                     /note="Ankyrin ubiquitin-binding domain; pfam18418"
                     /db_xref="CDD:408216"
     Region          357..541
                     /region_name="OTU_ZRANB1"
                     /note="OTU (ovarian tumor) domain of Ubiquitin
                     thioesterase ZRANB1 and similar proteins; cd22767"
                     /db_xref="CDD:438604"
     Site            order(394..395,456,463,470..472,494,534,536)
                     /site_type="other"
                     /note="putative polypeptide substrate binding site
                     [polypeptide binding]"
                     /db_xref="CDD:438604"
     CDS             1..655
                     /gene="trbd"
                     /coded_by="XM_077645656.1:252..2219"
                     /db_xref="GeneID:144112843"
ORIGIN      
        1 msrqgckwtc qhctyenwpk alrcvicqnp rptlieeeas seerdiykis plvspdltss
       61 yqkwacgrct ymnwprsskc tqclhprgsp cnaqasppss qpqqattste vehtsadlrs
      121 kwtcgactye nwpksrkcim cnatrskgds pvgnqtpdpd dlrntptgvp avctggsnrl
      181 tgtteievqm saeenppsnn ylcslrrqar emdrlwlnac ravvdgepep isrylnsggd
      241 parqlthaea gllartanfd vgftlahmai rqerddilal llsnsgmthp vakrvpsdvg
      301 rdaaadirrh iaasfkqrkg pfscyfvtef vtfalpteik efhssvqkvl fdelldqdvq
      361 keleedsaii nwsleltetl gsrlyalwnr sagnclldsa lqatwgvfdr dnslrqvlgd
      421 slseaasvly prwreaealq arllhfslde aqwqedwatl vsmaaqpgsa leqmhvftla
      481 hvlrrpivvy gvkyvksfrg ealgyarfeg iyipllweps fcwkspialg ytrghfsalv
      541 pmepepevig gaeanprgdl qvsflplmta drqllplhfl tqgevgreee iikqwldccv
      601 teggllvaqq kvprsphlvl qmvdqwleay rklsphhrlt dsqsgyssdg dsdqe
//
LOCUS       XP_077501783             626 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112844 [Amblyomma americanum].
ACCESSION   XP_077501783
VERSION     XP_077501783.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645657.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..626
                     /product="uncharacterized protein LOC144112844"
                     /calculated_mol_wt=68605
     CDS             1..626
                     /gene="LOC144112844"
                     /coded_by="XM_077645657.1:280..2160"
                     /db_xref="GeneID:144112844"
ORIGIN      
        1 mtspkkcssd arkyrtrhky rgkrrktvkk ttagndarda pasvrptadt ghrdsceida
       61 amdfvsasqk kmgffksdsr svgapaastv lcgvvccavl caltalvags acptcrerkl
      121 avreaaekrk glssylelrc enpdcpesvl sfthtskrii sagecgdpga ntrydsgssr
      181 dgfavnvkav laaraigagy dqlsrfcaii glrkplhhkt fhaiskklhg aameavrqnl
      241 eqarkvtkda vgggdvpvmf dgtwqkrghk shngvgtavs ldtglcldfe vlsnyclacs
      301 ihkpmgddde vwqafhrpvc ekntecssha mepeaavriw qrtlsyetpl rfttflsdgd
      361 skaytavcga evygdtviek eectnhvakr lgtalrkmtt plprgqklsv taiqklqtyy
      421 qiaitnnkgs vrsmytaiwa syfhccssdg asshkfcpae pdswckhrra ealgeptprh
      481 tplltkaqgl aimpiykrlt dekllarclh gktqnaaesl nskiwllcpk tkfasrtsve
      541 tataiavlwy nkghagfeev lqeigvlppe elvarsdhsd cmrikkmnlk rtaearahrr
      601 savkrartee tnrrsregps ysagaf
//
LOCUS       XP_077501784             221 aa            linear   INV 25-NOV-2025
DEFINITION  protein D2-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501784
VERSION     XP_077501784.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645658.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..221
                     /product="protein D2-like isoform X1"
                     /calculated_mol_wt=24431
     Region          61..205
                     /region_name="PEBP_euk"
                     /note="PhosphatidylEthanolamine-Binding Protein (PEBP)
                     domain present in eukaryotes; cd00866"
                     /db_xref="CDD:176644"
     Site            order(104,118,120,143..146,152,154)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:176644"
     CDS             1..221
                     /gene="LOC144112845"
                     /coded_by="XM_077645658.1:16..681"
                     /db_xref="GeneID:144112845"
ORIGIN      
        1 mdtnsgfvip pqgrqnnvvp asclrpnkpk lcvylqkgtg mtkmednqvv pdvidtvpkd
       61 tvevtyngvs vnmgntltpt qvqcpptvsy ptdagalytl cmtdpdapsr qtpkyrewhh
      121 wlvvnipgck vsdgetlsqy vgsgppkgtg lhryvfvvyk qpgklscdek rltnrsgdhr
      181 ggfkirdfak kyqlgepvaa nfyqaewddy vpklyeqlsg n
//
LOCUS       XP_077501785             234 aa            linear   INV 25-NOV-2025
DEFINITION  protein D3-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077501785
VERSION     XP_077501785.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645659.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..234
                     /product="protein D3-like isoform X2"
                     /calculated_mol_wt=25753
     Region          74..218
                     /region_name="PEBP_euk"
                     /note="PhosphatidylEthanolamine-Binding Protein (PEBP)
                     domain present in eukaryotes; cd00866"
                     /db_xref="CDD:176644"
     Site            order(117,131,133,156..159,165,167)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:176644"
     CDS             1..234
                     /gene="LOC144112845"
                     /coded_by="XM_077645659.1:3..707"
                     /db_xref="GeneID:144112845"
ORIGIN      
        1 mmfravcsra ifafgplsas ltssqraasa sgfsssfrrl lqqssvylqk gtgmtkmedn
       61 qvvpdvidtv pkdtvevtyn gvsvnmgntl tptqvqcppt vsyptdagal ytlcmtdpda
      121 psrqtpkyre whhwlvvnip gckvsdgetl sqyvgsgppk gtglhryvfv vykqpgklsc
      181 dekrltnrsg dhrggfkird fakkyqlgep vaanfyqaew ddyvpklyeq lsgn
//
LOCUS       XP_077501786             368 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112847 [Amblyomma americanum].
ACCESSION   XP_077501786
VERSION     XP_077501786.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645660.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..368
                     /product="uncharacterized protein LOC144112847"
                     /calculated_mol_wt=39614
     Region          7..98
                     /region_name="FHA"
                     /note="forkhead associated (FHA) domain superfamily;
                     cl00062"
                     /db_xref="CDD:469597"
     Region          206..>339
                     /region_name="PRK13335"
                     /note="superantigen-like protein SSL3; Reviewed"
                     /db_xref="CDD:139494"
     CDS             1..368
                     /gene="LOC144112847"
                     /coded_by="XM_077645660.1:58..1164"
                     /db_xref="GeneID:144112847"
ORIGIN      
        1 meyydpkivt lrkngtgfsv lplttecvsv ggaadcdiri pgsgshccff lvgkneqali
       61 incfgtadlk lngkdvhdys vlshgdivqi syrkfrfdyg ekqkkenssk vaaashqpka
      121 agdatptsnn kskkasteqa gavaceegdd eeegvplqdv tgsslvmast tqrkdvtfdk
      181 hlqreasptk evsvtrntra krkvdlkees iaekhttaed ligtvaeksg ghqqrvasge
      241 yapqendvqc snsakqvinp kitgdkevvd dapaspnvev atvepasagg kalattmepk
      301 rskrkmaavk vdvaqtanea tprktrgrra ealktalewa ddkpgasrrt akdrseeepp
      361 pkrtrkmv
//
LOCUS       XP_077501787             229 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112848 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501787
VERSION     XP_077501787.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645661.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..229
                     /product="uncharacterized protein LOC144112848 isoform X1"
                     /calculated_mol_wt=24493
     CDS             1..229
                     /gene="LOC144112848"
                     /coded_by="XM_077645661.1:603..1292"
                     /db_xref="GeneID:144112848"
ORIGIN      
        1 mdcsqspset sasvrragep aavsgepvsk gyvqsrssqs lllfqkdadg tsptgstlnq
       61 acshasgfim vqsdhtyagp steasassle pgtagvaatl sasadlspga pfsssplsce
      121 gsftspiner rplqklrakl rqlrnrnkrl qglllqrrrm kttaelqlrm qpaiprrirh
      181 ssktfpepwr hasvvlprss aassrcgglt ertksakasv rrrwcqrgs
//
LOCUS       XP_077501788             229 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112848 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501788
VERSION     XP_077501788.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645662.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..229
                     /product="uncharacterized protein LOC144112848 isoform X1"
                     /calculated_mol_wt=24493
     CDS             1..229
                     /gene="LOC144112848"
                     /coded_by="XM_077645662.1:1366..2055"
                     /db_xref="GeneID:144112848"
ORIGIN      
        1 mdcsqspset sasvrragep aavsgepvsk gyvqsrssqs lllfqkdadg tsptgstlnq
       61 acshasgfim vqsdhtyagp steasassle pgtagvaatl sasadlspga pfsssplsce
      121 gsftspiner rplqklrakl rqlrnrnkrl qglllqrrrm kttaelqlrm qpaiprrirh
      181 ssktfpepwr hasvvlprss aassrcgglt ertksakasv rrrwcqrgs
//
LOCUS       XP_077501789             229 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112848 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501789
VERSION     XP_077501789.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645663.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..229
                     /product="uncharacterized protein LOC144112848 isoform X1"
                     /calculated_mol_wt=24493
     CDS             1..229
                     /gene="LOC144112848"
                     /coded_by="XM_077645663.1:601..1290"
                     /db_xref="GeneID:144112848"
ORIGIN      
        1 mdcsqspset sasvrragep aavsgepvsk gyvqsrssqs lllfqkdadg tsptgstlnq
       61 acshasgfim vqsdhtyagp steasassle pgtagvaatl sasadlspga pfsssplsce
      121 gsftspiner rplqklrakl rqlrnrnkrl qglllqrrrm kttaelqlrm qpaiprrirh
      181 ssktfpepwr hasvvlprss aassrcgglt ertksakasv rrrwcqrgs
//
LOCUS       XP_077501790             218 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112848 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501790
VERSION     XP_077501790.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645664.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..218
                     /product="uncharacterized protein LOC144112848 isoform X2"
                     /calculated_mol_wt=23159
     CDS             1..218
                     /gene="LOC144112848"
                     /coded_by="XM_077645664.1:1369..2025"
                     /db_xref="GeneID:144112848"
ORIGIN      
        1 mdcsqspset sasvrragep aavsgepvsk gyvqsrssqs lllfqkdadg tsptgstlnq
       61 acshasgfim vqsdhtyagp steasassle pgtagvaatl sasadlspga pfsssplsce
      121 gsftspiner rplqklrakl rqlrnrnkrl qglllqrrrm kttaelkqaw idfvraagrh
      181 ywtpskytvl carytlttaa tnatchtspd safeqnvs
//
LOCUS       XP_077501791             216 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112848 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501791
VERSION     XP_077501791.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645665.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..216
                     /product="uncharacterized protein LOC144112848 isoform X3"
                     /calculated_mol_wt=23024
     CDS             1..216
                     /gene="LOC144112848"
                     /coded_by="XM_077645665.1:1364..2014"
                     /db_xref="GeneID:144112848"
ORIGIN      
        1 mdcsqspset sasvrragep aavsgepvsk gyvqsrssqs lllfqkdadg tsptgstlnq
       61 acshasgfim vqsdhtyagp steasassle pgtagvaatl sasadlspga pfsssplsce
      121 gsftspiner rplqklrakl rqlrnrnkrl qglllqrrrm kttaelvdsl rehltpavaa
      181 fveaqlkmhn vsrfgrrwcs qnktfalgly fhspkg
//
LOCUS       XP_077501792             119 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112850 [Amblyomma americanum].
ACCESSION   XP_077501792
VERSION     XP_077501792.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645666.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..119
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..119
                     /product="uncharacterized protein LOC144112850"
                     /calculated_mol_wt=12234
     CDS             1..119
                     /gene="LOC144112850"
                     /coded_by="XM_077645666.1:1993..2352"
                     /db_xref="GeneID:144112850"
ORIGIN      
        1 mrpvwlccss ggqlgtasgt aeklhvlhvg paknrtgcvp astvlargaq dspvnniaap
       61 repllqscth krrsmsvaag seeqpgtstg vgnllwfsfw afgklllsgg rdsgavfls
//
LOCUS       XP_077501794             830 aa            linear   INV 25-NOV-2025
DEFINITION  O-GlcNAcase isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501794
VERSION     XP_077501794.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645668.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..830
                     /product="O-GlcNAcase isoform X1"
                     /calculated_mol_wt=93167
     Region          23..292
                     /region_name="NAGidase"
                     /note="beta-N-acetylglucosaminidase; pfam07555"
                     /db_xref="CDD:429533"
     Region          <772..830
                     /region_name="RimI"
                     /note="Ribosomal protein S18 acetylase RimI and related
                     acetyltransferases [Translation, ribosomal structure and
                     biogenesis]; COG0456"
                     /db_xref="CDD:440224"
     CDS             1..830
                     /gene="Oga"
                     /coded_by="XM_077645668.1:284..2776"
                     /db_xref="GeneID:144112851"
ORIGIN      
        1 mgeetssvdp sgilnsrnhn lvcgvvegfy grpwtteqrk dlfqrmkklg lntymyapkd
       61 dfkhraywre lytveeaehl tnliqaaten sitfyyalsp glditysnsk evaclkrkle
      121 qvaqfgccaf allfddiepe isetdkevfq sfahaqvsvt nevyqelnqp kfifcpteyc
      181 aaravpnvqn seylntiglk lmpgidimwt ghkviskvit vqsiqeltev lrrppvlwdn
      241 lhandydqkr lflgpysgrs tdlvphlrgv lsnpnceyea nfvpvhtlaq wskcssdgkr
      301 dlnesvsadi rletegeaee mtshlgphty hprkalraal vdwlpefyrt ksaygrllsg
      361 aflggggapt ppsgsggpag shleeaasfq plsselvnsl vqspstplep mdchsateam
      421 qteepvddsr qlaledlall vdlfylpfeh ghqgvaflqe fhwlrsnghv vwerckraak
      481 dpegglaele ewrkraakfd qmtqavgrlf srltfvpnrs llydlypyiw dikgvislln
      541 syikwialgd vpcsstlafa tpsftwfskg ykeafisgeq epwlfrgglt aelqrllple
      601 svsdlflykp pespssktyt irpylpsdep lvyevcrktc ddgmdgtevf pefpnligdk
      661 cffkillvgg fltlsneycf vvedetgvcg yalaaldaqq fskklevawk pelclkypap
      721 vkepsemltp aeeimnsfhs rtitvpdivy khhpsrvtms mlpsimdssv skrllacvla
      781 alkangshgv fsevtmgdkn vvdfytklgf leialpefls eevfflgrtf
//
LOCUS       XP_077501795             824 aa            linear   INV 25-NOV-2025
DEFINITION  O-GlcNAcase isoform X2 [Amblyomma americanum].
ACCESSION   XP_077501795
VERSION     XP_077501795.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645669.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..824
                     /product="O-GlcNAcase isoform X2"
                     /calculated_mol_wt=92415
     Region          23..292
                     /region_name="NAGidase"
                     /note="beta-N-acetylglucosaminidase; pfam07555"
                     /db_xref="CDD:429533"
     Region          <766..824
                     /region_name="RimI"
                     /note="Ribosomal protein S18 acetylase RimI and related
                     acetyltransferases [Translation, ribosomal structure and
                     biogenesis]; COG0456"
                     /db_xref="CDD:440224"
     CDS             1..824
                     /gene="Oga"
                     /coded_by="XM_077645669.1:284..2758"
                     /db_xref="GeneID:144112851"
ORIGIN      
        1 mgeetssvdp sgilnsrnhn lvcgvvegfy grpwtteqrk dlfqrmkklg lntymyapkd
       61 dfkhraywre lytveeaehl tnliqaaten sitfyyalsp glditysnsk evaclkrkle
      121 qvaqfgccaf allfddiepe isetdkevfq sfahaqvsvt nevyqelnqp kfifcpteyc
      181 aaravpnvqn seylntiglk lmpgidimwt ghkviskvit vqsiqeltev lrrppvlwdn
      241 lhandydqkr lflgpysgrs tdlvphlrgv lsnpnceyea nfvpvhtlaq wskcssdgkr
      301 dlnesvsadi rletegeaee mtshlgphty hprkalraal vdwlpefyrt ksaygrllsg
      361 aflggggapt ppsgsggpag shleeaasfq plsselvnsl vqspstplep mdchsateam
      421 qteepvddsr qlaledlall vdlfylpfeh ghqgvaflqe fhwlrsnghv vwerckraak
      481 dpegglaele ewrkraakfd qmtqavgrlf srltfvpnrs llydlypyiw dikgvislln
      541 syikwialgd vpcsstlafa tpsftwfskg ykeafisgeq epwlfrgglt aelqrllple
      601 svsdlflykp pespssktyt irpylpsdep lvyevcrktc ddgmdgtevf pefpnligdk
      661 lvggfltlsn eycfvvedet gvcgyalaal daqqfskkle vawkpelclk ypapvkepse
      721 mltpaeeimn sfhsrtitvp divykhhpsr vtmsmlpsim dssvskrlla cvlaalkang
      781 shgvfsevtm gdknvvdfyt klgfleialp eflseevffl grtf
//
LOCUS       XP_077501796             811 aa            linear   INV 25-NOV-2025
DEFINITION  O-GlcNAcase isoform X3 [Amblyomma americanum].
ACCESSION   XP_077501796
VERSION     XP_077501796.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645670.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..811
                     /product="O-GlcNAcase isoform X3"
                     /calculated_mol_wt=91171
     Region          23..292
                     /region_name="NAGidase"
                     /note="beta-N-acetylglucosaminidase; pfam07555"
                     /db_xref="CDD:429533"
     Region          <753..811
                     /region_name="RimI"
                     /note="Ribosomal protein S18 acetylase RimI and related
                     acetyltransferases [Translation, ribosomal structure and
                     biogenesis]; COG0456"
                     /db_xref="CDD:440224"
     CDS             1..811
                     /gene="Oga"
                     /coded_by="XM_077645670.1:253..2688"
                     /db_xref="GeneID:144112851"
ORIGIN      
        1 mgeetssvdp sgilnsrnhn lvcgvvegfy grpwtteqrk dlfqrmkklg lntymyapkd
       61 dfkhraywre lytveeaehl tnliqaaten sitfyyalsp glditysnsk evaclkrkle
      121 qvaqfgccaf allfddiepe isetdkevfq sfahaqvsvt nevyqelnqp kfifcpteyc
      181 aaravpnvqn seylntiglk lmpgidimwt ghkviskvit vqsiqeltev lrrppvlwdn
      241 lhandydqkr lflgpysgrs tdlvphlrgv lsnpnceyea nfvpvhtlaq wskcssdgkr
      301 dlnesvsadi rletegeaee mtshlgphty hprkalraal vdwlpefyrt ksaygrllsg
      361 aflggggapt ppsgsggpag shleeaasfq plsselvnsl vqspstplep mdchsateam
      421 qteepvddsr qlaledlall vdlfylpfeh ghqgvaflqe fhwlrsnghv vwerckraak
      481 dpegglaele ewrkraakfd qmtqavgrlf srltfvpnrs llydlypyiw dikgvislln
      541 syikwigfsk gykeafisge qepwlfrggl taelqrllpl esvsdlflyk ppespsskty
      601 tirpylpsde plvyevcrkt cddgmdgtev fpefpnligd kcffkillvg gfltlsneyc
      661 fvvedetgvc gyalaaldaq qfskklevaw kpelclkypa pvkepsemlt paeeimnsfh
      721 srtitvpdiv ykhhpsrvtm smlpsimdss vskrllacvl aalkangshg vfsevtmgdk
      781 nvvdfytklg fleialpefl seevfflgrt f
//
LOCUS       XP_077501797             805 aa            linear   INV 25-NOV-2025
DEFINITION  O-GlcNAcase isoform X4 [Amblyomma americanum].
ACCESSION   XP_077501797
VERSION     XP_077501797.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645671.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..805
                     /product="O-GlcNAcase isoform X4"
                     /calculated_mol_wt=90419
     Region          23..292
                     /region_name="NAGidase"
                     /note="beta-N-acetylglucosaminidase; pfam07555"
                     /db_xref="CDD:429533"
     Region          <747..805
                     /region_name="RimI"
                     /note="Ribosomal protein S18 acetylase RimI and related
                     acetyltransferases [Translation, ribosomal structure and
                     biogenesis]; COG0456"
                     /db_xref="CDD:440224"
     CDS             1..805
                     /gene="Oga"
                     /coded_by="XM_077645671.1:264..2681"
                     /db_xref="GeneID:144112851"
ORIGIN      
        1 mgeetssvdp sgilnsrnhn lvcgvvegfy grpwtteqrk dlfqrmkklg lntymyapkd
       61 dfkhraywre lytveeaehl tnliqaaten sitfyyalsp glditysnsk evaclkrkle
      121 qvaqfgccaf allfddiepe isetdkevfq sfahaqvsvt nevyqelnqp kfifcpteyc
      181 aaravpnvqn seylntiglk lmpgidimwt ghkviskvit vqsiqeltev lrrppvlwdn
      241 lhandydqkr lflgpysgrs tdlvphlrgv lsnpnceyea nfvpvhtlaq wskcssdgkr
      301 dlnesvsadi rletegeaee mtshlgphty hprkalraal vdwlpefyrt ksaygrllsg
      361 aflggggapt ppsgsggpag shleeaasfq plsselvnsl vqspstplep mdchsateam
      421 qteepvddsr qlaledlall vdlfylpfeh ghqgvaflqe fhwlrsnghv vwerckraak
      481 dpegglaele ewrkraakfd qmtqavgrlf srltfvpnrs llydlypyiw dikgvislln
      541 syikwigfsk gykeafisge qepwlfrggl taelqrllpl esvsdlflyk ppespsskty
      601 tirpylpsde plvyevcrkt cddgmdgtev fpefpnligd klvggfltls neycfvvede
      661 tgvcgyalaa ldaqqfskkl evawkpelcl kypapvkeps emltpaeeim nsfhsrtitv
      721 pdivykhhps rvtmsmlpsi mdssvskrll acvlaalkan gshgvfsevt mgdknvvdfy
      781 tklgfleial peflseevff lgrtf
//
LOCUS       XP_077501798             507 aa            linear   INV 25-NOV-2025
DEFINITION  putative GTP-binding protein 6 [Amblyomma americanum].
ACCESSION   XP_077501798
VERSION     XP_077501798.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645672.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..507
                     /product="putative GTP-binding protein 6"
                     /calculated_mol_wt=57336
     Region          86..502
                     /region_name="HflX"
                     /note="50S ribosomal subunit-associated GTPase HflX
                     [Translation, ribosomal structure and biogenesis];
                     COG2262"
                     /db_xref="CDD:441863"
     CDS             1..507
                     /gene="LOC144112852"
                     /coded_by="XM_077645672.1:157..1680"
                     /db_xref="GeneID:144112852"
ORIGIN      
        1 mvllfsvshl arrispilph rcsvrdfpla lrrrtcacrs aslvilrrcs teadyiefen
       61 fvhcssyveq qpkfsllnlp cgghdvlvvq prikkgrrir tdtttalqla eavalvetlp
      121 gwraagrltl ktdndmrkli fktgnleriq dsvkeksasa vfinvdiltg vqhvalqeff
      181 grpiydrytv vlnifrhhar treaklqial aeipyyrari whihkgkgrd msgtgqtyye
      241 rqqqqlasre aalrrqlgel egersllrkk rrqleyptva vvgytnagkt aligqltddn
      301 rlqprdqlfa tldvtahagr lraiktvlym dtvgfisdip talvasfast ledvvladvi
      361 vhvrdlshpd rdaqrnnvle tlerigvpsk llnsiievgn kidllqsppt sseqcvpkyl
      421 tscvdgrglp elreiiehkl ientgrtvvr frvptggpey twlykegtfl sctadehdwn
      481 ysfidavltp aavakfrhhf gnwcime
//
LOCUS       XP_077501799             407 aa            linear   INV 25-NOV-2025
DEFINITION  glutamate oxaloacetate transaminase 1 [Amblyomma americanum].
ACCESSION   XP_077501799
VERSION     XP_077501799.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645673.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..407
                     /product="glutamate oxaloacetate transaminase 1"
                     /calculated_mol_wt=45649
     Region          1..403
                     /region_name="AAT_I"
                     /note="Aspartate aminotransferase (AAT) superfamily (fold
                     type I) of pyridoxal phosphate (PLP)-dependent enzymes.
                     PLP combines with an alpha-amino acid to form a compound
                     called a Schiff base or aldimine intermediate, which
                     depending on the reaction, is the...; cl18945"
                     /db_xref="CDD:450240"
     Site            order(105..107,133,187,218,248,250..251,259)
                     /site_type="other"
                     /note="pyridoxal 5'-phosphate binding site [chemical
                     binding]"
                     /db_xref="CDD:99734"
     Site            order(108,143,211,257..259,295,298)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:99734"
     Site            251
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:99734"
     CDS             1..407
                     /gene="Got1"
                     /coded_by="XM_077645673.1:111..1334"
                     /db_xref="GeneID:144112853"
ORIGIN      
        1 mtrfscveaa ppveifalmr ayradtfaqk vdlgvgayrt eeakpwvlpv vrkvekemae
       61 dtslnheylg qlglddfska aihmllgnen qaikegravg vqclsgtgsl rvgadllckh
      121 akfttvymst ptwpnhalvf khsgfqnlkh yrywdaknrr ldfdgmiedl qnapedsvvv
      181 lhacahnptg idptedqwkk iaevikakkl fpffdcayqg fasgdlekds wairyfvslg
      241 felvcaqsfa knfglyneri gnlllvindk aaltnvlaqi tllvrgnysn ppnhgarivs
      301 kvlntpeyfe ewkghiqtma nriiamrkal qdklhelgtp gswehitkqi gmfsytglnq
      361 qqvqhlvqey hvylpkdgri sicglntgnv eyvakaihda vtkfpqs
//
LOCUS       XP_077501800             458 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112854 [Amblyomma americanum].
ACCESSION   XP_077501800
VERSION     XP_077501800.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645674.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..458
                     /product="uncharacterized protein LOC144112854"
                     /calculated_mol_wt=51993
     Region          <1..63
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..458
                     /gene="LOC144112854"
                     /coded_by="XM_077645674.1:105..1481"
                     /db_xref="GeneID:144112854"
ORIGIN      
        1 mlakwsraip radkaleens avcerhfdsr fivrdfkhii ngaevliprs ipalsedavp
       61 tvfpnlpkyi srmlpkerkr rnasqapips krsgacldtt eecppadveg adtqrggmse
      121 pfaeelfifd lkrpselwsl qklkqnniac yqtaefakge tppvvftkmv vfcvgeghvk
      181 csiflagqfh kecdvrckeq aegelsyans ltvcsgight kefeklnmnn igrlsghclf
      241 ssacngsvpq rtsaqlpnrc lsckksrssm karllrckkk arlqlsnvaa krlkyamrtl
      301 qrrnnklnsl qdalkkmqek nmaikatffe ekvtlntlil ylksiyfcns vyffqlkelp
      361 kkqqpavrac fevsrrksth gyrynkewll ecillrmksp rlyehlrshk ilvlpsrvcl
      421 qrymkgfkas ygfstkllgc vkkkqkslme lgamgacs
//
LOCUS       XP_077501801             367 aa            linear   INV 25-NOV-2025
DEFINITION  cytosolic 5'-nucleotidase IIIB isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501801
VERSION     XP_077501801.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645675.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..367
                     /product="cytosolic 5'-nucleotidase IIIB isoform X1"
                     /calculated_mol_wt=41368
     Region          94..365
                     /region_name="HAD_like"
                     /note="Haloacid Dehalogenase-like Hydrolases; cl21460"
                     /db_xref="CDD:473868"
     Site            order(118..122,233..234,282,306..307,310..311)
                     /site_type="active"
                     /db_xref="CDD:319763"
     CDS             1..367
                     /gene="cN-IIIB"
                     /coded_by="XM_077645675.1:131..1234"
                     /db_xref="GeneID:144112860"
ORIGIN      
        1 mvapdkekte ivsqssspys vlatklqqlf paclksrvem srktvvmcta lgigtvvvvs
       61 faatallrkr krakakrvid tvlelqkpcv rmkdpervee lvcklikggp kklqvvtdfd
      121 htisrshyng kpcrsthsvl esssliseey rlqaqrihdk yhpieidpnm staekiphmv
      181 ewysrshail vncgvkksdi pelvagssim lrdgcesffe mlhehqiptl vfsaglgdil
      241 eealrhfqch fpnlkfisny mqfdeegnit gfkgelihmy nknschmtsp eyhqavkekt
      301 nivllgdslg didmlagghl qdvvlrigfl ntkieerlpq flnsfdivll ddqtmdvvng
      361 ilrkiiy
//
LOCUS       XP_077501802             307 aa            linear   INV 25-NOV-2025
DEFINITION  cytosolic 5'-nucleotidase IIIB isoform X2 [Amblyomma americanum].
ACCESSION   XP_077501802
VERSION     XP_077501802.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645676.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..307
                     /product="cytosolic 5'-nucleotidase IIIB isoform X2"
                     /calculated_mol_wt=34947
     Region          34..305
                     /region_name="HAD_like"
                     /note="Haloacid Dehalogenase-like Hydrolases; cl21460"
                     /db_xref="CDD:473868"
     Site            order(58..62,173..174,222,246..247,250..251)
                     /site_type="active"
                     /db_xref="CDD:319763"
     CDS             1..307
                     /gene="cN-IIIB"
                     /coded_by="XM_077645676.1:35..958"
                     /db_xref="GeneID:144112860"
ORIGIN      
        1 mtgvendlhr drdhclssly gvlelqkpcv rmkdpervee lvcklikggp kklqvvtdfd
       61 htisrshyng kpcrsthsvl esssliseey rlqaqrihdk yhpieidpnm staekiphmv
      121 ewysrshail vncgvkksdi pelvagssim lrdgcesffe mlhehqiptl vfsaglgdil
      181 eealrhfqch fpnlkfisny mqfdeegnit gfkgelihmy nknschmtsp eyhqavkekt
      241 nivllgdslg didmlagghl qdvvlrigfl ntkieerlpq flnsfdivll ddqtmdvvng
      301 ilrkiiy
//
LOCUS       XP_077501803             158 aa            linear   INV 25-NOV-2025
DEFINITION  POP5 ribonuclease P/MRP subunit [Amblyomma americanum].
ACCESSION   XP_077501803
VERSION     XP_077501803.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645677.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="POP5 ribonuclease P/MRP subunit"
                     /calculated_mol_wt=18172
     Region          7..114
                     /region_name="RNase_P_Rpp14"
                     /note="Rpp14/Pop5 family; pfam01900"
                     /db_xref="CDD:460379"
     CDS             1..158
                     /gene="Pop5"
                     /coded_by="XM_077645677.1:98..574"
                     /db_xref="GeneID:144112861"
ORIGIN      
        1 mvrlkhryvl veilwkdwqd phifelpvae kdlyicvrka vnflhgdfgv gvtkfnlaik
       61 ffnphtrvfl lrtrrgahtl tlsalpfvkk ikneaatlrv lkltgsmrsc lkylkkydcn
      121 vmlsvlnrcd sveakrkfre tiakvydsme skggarps
//
LOCUS       XP_077501804             545 aa            linear   INV 25-NOV-2025
DEFINITION  nop56 ribonucleoprotein [Amblyomma americanum].
ACCESSION   XP_077501804
VERSION     XP_077501804.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645678.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..545
                     /product="nop56 ribonucleoprotein"
                     /calculated_mol_wt=60781
     Region          5..70
                     /region_name="NOP5NT"
                     /note="NOP5NT (NUC127) domain; pfam08156"
                     /db_xref="CDD:462382"
     Region          177..408
                     /region_name="Nop"
                     /note="snoRNA binding domain, fibrillarin; pfam01798"
                     /db_xref="CDD:460335"
     CDS             1..545
                     /gene="Nop56"
                     /coded_by="XM_077645678.1:151..1788"
                     /db_xref="GeneID:144112862"
ORIGIN      
        1 msqlyvlfeh avgyalfrvk efeevsmalp qveksvldcn kfkslvhlia flpfrtavna
       61 lenmnclteg vvhedlqtfl etnlpkstkk skvmlgvgds klgmtiqesm giscqhtgvv
      121 pevirglrlh fskivkgltd assskaqlgl ghsysrskvk fnvnridnmi iqsialldql
      181 dkdintfamr vrewysyhfp elvkivpdny tyaktamlik nrkdlsedsl ealeevvmds
      241 akaqaiidaa rasmgmdisp vdliniemfa srvtslveyr kelmeylrsk mhdiapnlat
      301 ligetvgarl ishagsltnl akypastvqi lgaekalfra lktrgntpky glifhstyig
      361 ragtknkgri srylankcsl asridcfsdm ptnifglklk eqvedrlkfy etgdiprknv
      421 dvmtdalaea qaaaaalrkl kkkekkkkkk dlensamdtt veaeeqenie veppkkkkkk
      481 gavenstgdt aveegqdaee apkkkkkkkh anvedeykev eqecngnade gslekpkkkk
      541 skaqk
//
LOCUS       XP_077501805             238 aa            linear   INV 25-NOV-2025
DEFINITION  polycomb group ring finger 3-like lethal (3) 73Ah [Amblyomma
            americanum].
ACCESSION   XP_077501805
VERSION     XP_077501805.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645679.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..238
                     /product="polycomb group ring finger 3-like lethal (3)
                     73Ah"
                     /calculated_mol_wt=28026
     Region          15..80
                     /region_name="RING-HC_PCGF3"
                     /note="RING finger found in polycomb group RING finger
                     protein 3 (PCGF3) and similar proteins; cd16735"
                     /db_xref="CDD:438393"
     Site            order(27,31,33..34,37,39..40,42..46,49..52,54..55)
                     /site_type="other"
                     /note="putative heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:438393"
     Region          149..233
                     /region_name="RAWUL_PCGF3"
                     /note="RING finger- and WD40-associated ubiquitin-like
                     (RAWUL) domain found in polycomb group RING finger protein
                     3 (PCGF3) and similar proteins; cd17083"
                     /db_xref="CDD:340603"
     CDS             1..238
                     /gene="l(3)73Ah"
                     /coded_by="XM_077645679.1:242..958"
                     /db_xref="GeneID:144112863"
ORIGIN      
        1 mvfipqesls rqmerrirlk tlnnhiicki ckgylidatt vteclhtfck sclvkhledn
       61 ntcptceivi hqsyplqyis ydrtmqdivy klvpnlqyne mkrerdfyrr kglpcpkaht
      121 pgmlnekdag skassskede adchrsdeqv nillesdsvq lkqmrrkfir cssqatithl
      181 kkfiakklfn nldrykevei lcnqeilgkd htlkfvyvtr wrfkdpplkl yyrpkvel
//
LOCUS       XP_077501806             238 aa            linear   INV 25-NOV-2025
DEFINITION  polycomb group ring finger 3-like lethal (3) 73Ah [Amblyomma
            americanum].
ACCESSION   XP_077501806
VERSION     XP_077501806.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645680.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..238
                     /product="polycomb group ring finger 3-like lethal (3)
                     73Ah"
                     /calculated_mol_wt=28026
     Region          15..80
                     /region_name="RING-HC_PCGF3"
                     /note="RING finger found in polycomb group RING finger
                     protein 3 (PCGF3) and similar proteins; cd16735"
                     /db_xref="CDD:438393"
     Site            order(27,31,33..34,37,39..40,42..46,49..52,54..55)
                     /site_type="other"
                     /note="putative heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:438393"
     Region          149..233
                     /region_name="RAWUL_PCGF3"
                     /note="RING finger- and WD40-associated ubiquitin-like
                     (RAWUL) domain found in polycomb group RING finger protein
                     3 (PCGF3) and similar proteins; cd17083"
                     /db_xref="CDD:340603"
     CDS             1..238
                     /gene="l(3)73Ah"
                     /coded_by="XM_077645680.1:134..850"
                     /db_xref="GeneID:144112863"
ORIGIN      
        1 mvfipqesls rqmerrirlk tlnnhiicki ckgylidatt vteclhtfck sclvkhledn
       61 ntcptceivi hqsyplqyis ydrtmqdivy klvpnlqyne mkrerdfyrr kglpcpkaht
      121 pgmlnekdag skassskede adchrsdeqv nillesdsvq lkqmrrkfir cssqatithl
      181 kkfiakklfn nldrykevei lcnqeilgkd htlkfvyvtr wrfkdpplkl yyrpkvel
//
LOCUS       XP_077501807            3543 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112865 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501807
VERSION     XP_077501807.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645681.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..3543
                     /product="uncharacterized protein LOC144112865 isoform X1"
                     /calculated_mol_wt=389534
     Region          135..237
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          212..239
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <340..>397
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          354..387
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <1487..1567
                     /region_name="B41"
                     /note="Band 4.1 homologues; smart00295"
                     /db_xref="CDD:214604"
     Region          1702..1937
                     /region_name="RasGEF"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     small GTPases. Small GTP-binding proteins of the Ras
                     superfamily function as molecular switches in fundamental
                     events such as signal transduction, cytoskeleton dynamics
                     and intracellular trafficking; cl02485"
                     /db_xref="CDD:470590"
     Site            order(1734..1736,1753..1754,1756..1758,1760..1761,
                     1764..1765,1768,1801,1804..1805,1807..1809,1811..1812,
                     1813..1814,1838,1841,1846..1848,1851,1865..1867,
                     1870..1872,1874..1876,1878..1881)
                     /site_type="other"
                     /note="Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:238087"
     Region          2343..2509
                     /region_name="EFh_PI-PLC"
                     /note="EF-hand motif found in eukaryotic
                     phosphoinositide-specific phospholipase C (PI-PLC, EC
                     3.1.4.11) isozymes; cl28895"
                     /db_xref="CDD:333715"
     Region          2344..2369
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320029"
     Region          2375..2427
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320029"
     Region          2436..2471
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320029"
     Region          2460..3135
                     /region_name="PLN02952"
                     /note="phosphoinositide phospholipase C"
                     /db_xref="CDD:178538"
     Region          2476..2509
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320029"
     Region          2552..2980
                     /region_name="PI-PLCc_epsilon"
                     /note="Catalytic domain of metazoan
                     phosphoinositide-specific phospholipase C-epsilon;
                     cd08596"
                     /db_xref="CDD:176538"
     Site            order(2567..2568,2597,2599,2612,2646,2698,2700,2906,2933,
                     2935)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:176538"
     Site            order(2567,2612)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:176538"
     Site            order(2568,2597,2599,2646)
                     /site_type="other"
                     /note="putative Ca binding site [ion binding]"
                     /db_xref="CDD:176538"
     Region          3187..3284
                     /region_name="RA_PLC-epsilon"
                     /note="Ras-associating (RA) domain found in
                     Phosphatidylinositide-specific phospholipase C
                     (PLC)-epsilon; cd17114"
                     /db_xref="CDD:340634"
     Region          3323..3429
                     /region_name="Ubl1_cv_Nsp3_N-like"
                     /note="first ubiquitin-like (Ubl) domain located at the
                     N-terminus of coronavirus SARS-CoV non-structural protein
                     3 (Nsp3) and related proteins; cl28922"
                     /db_xref="CDD:475130"
     CDS             1..3543
                     /gene="LOC144112865"
                     /coded_by="XM_077645681.1:252..10883"
                     /db_xref="GeneID:144112865"
ORIGIN      
        1 mtswaamsgs dspprepepp pecsssagss asplhashqy rfglhndlgn ftakppkiyp
       61 eaggeptsgg ggpqrspapl vavqstptpp psedhlpsgg gslplsasch sltaattgss
      121 lgssindltl eaeqlerair andvaatrrl lelhhgrfsv nlhgslldks sagsasqdme
      181 illrksqtli drfgrgdsvs tdlepvpavf asalhlaveh qaldvvslll kygvepneag
      241 vplgslrrgs satseasgsl rratlsprgg gpeqqpspel lrrrkyvplr pelaatlrvv
      301 rhsadgreit feeeytrdfl yslpplflaa algrtaaarl llkygaaasp rdkngvtplh
      361 laacqpqpaw pclrllleag arihvasqrg atpcdlaetd lsavqislve aafdgaqaaa
      421 taaaaalqta spgpgaasav paaatstapa appakpppee gahaprsnil rrlqdsrpgq
      481 thrgsarkke ppdelapspd gapgrqrspg vltatpdedk pqervpsaep qcsklnrrks
      541 gddgrlkakc ntpsveevep anqkvdpsqk vdaslaaltr mahnpeclds ilaglqrhlg
      601 aiieltllvd gerlqrplaa lfnrilltvt seqsepgdpa vdddtvaahm cqllrvalss
      661 lrggqalqft alllinklvd lgvarglalr psrsratgrc sahrlaalwh qssslkenha
      721 fqplpsvfpw kvpkapdppp grwwwawrsg grdpaadaep psratrpsfl ailadegvdf
      781 llnmlnnait lhkrvvgtrh hctpshrwrh csyhclqils aravlfmsqn tevqnklvee
      841 ahlkilvnal dsthdpqllc lvlqivatla lvpdhhtalt daelpdsltq lvlpsdewyy
      901 tnhstkyary vkhhaarvlv ylgmeqrlrn kvylfdlied qavpstpmld sgedgyivdt
      961 slppcavyds ahrlvgmsve tfvldmlksa esltcgvekk aapqqqksfl dfcldpvssy
     1021 sqeasgvhfh lsalqmvlpp vivvrllqhr llgtahavrr gapsrasias enrsragssa
     1081 dteeslrerr hialtvncap pgppswtras sveerresir hadikqvtvv apdggpepsp
     1141 asspagrrtf kfsslkkras kaptasqdqp srttseadia afqkelqnlp nfetlagssd
     1201 afpeggaggl dgagyarprs csvprvtfea aslqlphghg plirsatteg aytgssaskp
     1261 aaaaappppt vsvpqpvqqs llsaceavap ahgplcelpg ahliilklly ewtcicpgdl
     1321 rirefrdfvn rlacmgdayk ewadelrria dlkeldkddv kpddleaihd eysklrdlvv
     1381 ngdllcskee aavlagvqlr ieetwpsnar trhvlgseql gklvpiteda keglipeppg
     1441 pgesssrmss aeaddspaes sdgagepatp tsrssgsflr vvpkrrslil rqwigsngnr
     1501 sptglhnavs lkeclppcyh taknigktik eqkrklfhsp lyeseihlkr lyiqtckrlp
     1561 sygchvfqvk ellrgktkkr ahrvlgigie hvvlldsktl vlaksqhtse lqqwragggr
     1621 shdrlvlefr gtkwsfvaaq slrsissvlw eimqdldarf ldehlittre eldkeshrnm
     1681 vsvhglerat iykeelerlq gllhfpeeva vrltdveyel fysvspvhyi rqvtvdlsgk
     1741 aatfnagekd rtvqalikrf nevsswvthi iisqpthedr kavlscilri amccwnignf
     1801 ntamevlagl kseklkpfwl slsdkdqlpv leflsqalln pepseeyraa veralniphs
     1861 kvipffgtfl rdlrailqgm pslvvlpsek akkiefvsdf hgedhfmtri gvggiinvek
     1921 ihqahrvldd ikafhhhykc rehalaqvai nskevrreet sckeddslyl qdpeayipiq
     1981 elandhhism vpltvdrvdf hqlqilhhgc tmvhweedgs rsslcyvrle rsnatitwck
     2041 pawsalrgsg pqdyalsvni eenvpvglsq kydtgesyla gleegfldln vvkeivlcrs
     2101 sievaavakr hgledsfsdq nllrfkfgag lsenrvlefi lpnflsviwy dslrrlvsml
     2161 kqqrqlcdgr ihwlketylq lyfreqscig ptpaeaikvf ggrkwtlnsl gssssvests
     2221 safkrassfg msttklrkkk sstslatvrd vgpkclpevd dggisarkqs ptikkrratv
     2281 ksppqlrsqe cspqrtsvsr sssscenlnr teggipivhp ttlatgyker scqvaastsk
     2341 assllsrpai thssqmnfle faelfrsfmv rcrkdlkdif esvatnskvt agelaaeeta
     2401 spksartrpk vlglltrssr feyvdpeqkk icdaiatasi vsncagvdss krlvlecadf
     2461 rkflatrqke dlteeevirl iqrhepdnvl reknllsfeg farylldkdn yafvpeiipp
     2521 gantvrlsyi rfyssrhdsv tftvlrhvfv sqnmdkplsh yyiasshnty ltghqlkges
     2581 svelysevll rgcrcveldc wdgddgipvi yhghtltski pfksvveaih kssfatspfp
     2641 iilsienhcs lpqqakmaqi ftnvfgerlq tqflfsndvc dearlpspnq lrykvliknk
     2701 klrppltpal plksakgvpq gkiapgrtns iistastgsl ndedeedyed ydeeddnlee
     2761 slssqegsck eamrhpgtls pgaahprprn padaehlhsd ddgykqrkss sqiapelsdl
     2821 vtycqaikfr gfvtcpsptn svkvkkvssk knvlasagsp lgvntplqts epkhdtptas
     2881 tasgsisaps stkrhcsnsv cfqvssmnes aakrlckrhp lalithcenq lmrtypagmr
     2941 idssnfnpvt fwafglqmva lnyqtedagl hintamfeqn gscgyvlkps amwdknnimh
     3001 gqynpwdkef dgihplnlhi tvisgqyvcq ntyacspqve vevvgvpldc srqktkivqr
     3061 nslnpiwqds fcfriafael aflrfsvldv mtnhilsqrv ipvkslkqgy rhvrlrspqn
     3121 qplplsslfv ysefeeegle vvqqngdlle gaipkkakgk akelstaesv rcdgrdnlva
     3181 ssqglkrrmf flvvhgvmpd epktilkite dttsreviaq alqkankpnd slddyvlmee
     3241 tqknwdritq eksttqrild pdecpllsqt tgantesrfi lkkladdpst rawmttikss
     3301 snkerrkaes gasgglsews dgeetylvcv ynvsadqpya ilkapissta qdiiaqalvk
     3361 arraedpinf vlveqmeyps pltesapasg ssvrrrglsr grrtlfdden vyevqaqwka
     3421 rgwfelrern eglserrkts apksssfyrl srlrssmksk erdkscpspk qspdgrrsgg
     3481 pstsaaaagg agasarepct sreaqeprqv hsegealsdd dappaamaar lrkisfrklk
     3541 vwr
//
LOCUS       XP_077501808            3515 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112865 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501808
VERSION     XP_077501808.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645682.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..3515
                     /product="uncharacterized protein LOC144112865 isoform X2"
                     /calculated_mol_wt=386151
     Region          135..237
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          212..239
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <340..>397
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          354..387
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <1487..1567
                     /region_name="B41"
                     /note="Band 4.1 homologues; smart00295"
                     /db_xref="CDD:214604"
     Region          1702..1937
                     /region_name="RasGEF"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     small GTPases. Small GTP-binding proteins of the Ras
                     superfamily function as molecular switches in fundamental
                     events such as signal transduction, cytoskeleton dynamics
                     and intracellular trafficking; cl02485"
                     /db_xref="CDD:470590"
     Site            order(1734..1736,1753..1754,1756..1758,1760..1761,
                     1764..1765,1768,1801,1804..1805,1807..1809,1811..1812,
                     1813..1814,1838,1841,1846..1848,1851,1865..1867,
                     1870..1872,1874..1876,1878..1881)
                     /site_type="other"
                     /note="Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:238087"
     Region          2343..2509
                     /region_name="EFh_PI-PLC"
                     /note="EF-hand motif found in eukaryotic
                     phosphoinositide-specific phospholipase C (PI-PLC, EC
                     3.1.4.11) isozymes; cl28895"
                     /db_xref="CDD:333715"
     Region          2344..2369
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320029"
     Region          2375..2427
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320029"
     Region          2436..2471
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320029"
     Region          2460..3107
                     /region_name="PLN02952"
                     /note="phosphoinositide phospholipase C"
                     /db_xref="CDD:178538"
     Region          2476..2509
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320029"
     Region          2525..2952
                     /region_name="PI-PLCc_epsilon"
                     /note="Catalytic domain of metazoan
                     phosphoinositide-specific phospholipase C-epsilon;
                     cd08596"
                     /db_xref="CDD:176538"
     Site            order(2539..2540,2569,2571,2584,2618,2670,2672,2878,2905,
                     2907)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:176538"
     Site            order(2539,2584)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:176538"
     Site            order(2540,2569,2571,2618)
                     /site_type="other"
                     /note="putative Ca binding site [ion binding]"
                     /db_xref="CDD:176538"
     Region          3159..3256
                     /region_name="RA_PLC-epsilon"
                     /note="Ras-associating (RA) domain found in
                     Phosphatidylinositide-specific phospholipase C
                     (PLC)-epsilon; cd17114"
                     /db_xref="CDD:340634"
     Region          3295..3401
                     /region_name="Ubl1_cv_Nsp3_N-like"
                     /note="first ubiquitin-like (Ubl) domain located at the
                     N-terminus of coronavirus SARS-CoV non-structural protein
                     3 (Nsp3) and related proteins; cl28922"
                     /db_xref="CDD:475130"
     CDS             1..3515
                     /gene="LOC144112865"
                     /coded_by="XM_077645682.1:252..10799"
                     /db_xref="GeneID:144112865"
ORIGIN      
        1 mtswaamsgs dspprepepp pecsssagss asplhashqy rfglhndlgn ftakppkiyp
       61 eaggeptsgg ggpqrspapl vavqstptpp psedhlpsgg gslplsasch sltaattgss
      121 lgssindltl eaeqlerair andvaatrrl lelhhgrfsv nlhgslldks sagsasqdme
      181 illrksqtli drfgrgdsvs tdlepvpavf asalhlaveh qaldvvslll kygvepneag
      241 vplgslrrgs satseasgsl rratlsprgg gpeqqpspel lrrrkyvplr pelaatlrvv
      301 rhsadgreit feeeytrdfl yslpplflaa algrtaaarl llkygaaasp rdkngvtplh
      361 laacqpqpaw pclrllleag arihvasqrg atpcdlaetd lsavqislve aafdgaqaaa
      421 taaaaalqta spgpgaasav paaatstapa appakpppee gahaprsnil rrlqdsrpgq
      481 thrgsarkke ppdelapspd gapgrqrspg vltatpdedk pqervpsaep qcsklnrrks
      541 gddgrlkakc ntpsveevep anqkvdpsqk vdaslaaltr mahnpeclds ilaglqrhlg
      601 aiieltllvd gerlqrplaa lfnrilltvt seqsepgdpa vdddtvaahm cqllrvalss
      661 lrggqalqft alllinklvd lgvarglalr psrsratgrc sahrlaalwh qssslkenha
      721 fqplpsvfpw kvpkapdppp grwwwawrsg grdpaadaep psratrpsfl ailadegvdf
      781 llnmlnnait lhkrvvgtrh hctpshrwrh csyhclqils aravlfmsqn tevqnklvee
      841 ahlkilvnal dsthdpqllc lvlqivatla lvpdhhtalt daelpdsltq lvlpsdewyy
      901 tnhstkyary vkhhaarvlv ylgmeqrlrn kvylfdlied qavpstpmld sgedgyivdt
      961 slppcavyds ahrlvgmsve tfvldmlksa esltcgvekk aapqqqksfl dfcldpvssy
     1021 sqeasgvhfh lsalqmvlpp vivvrllqhr llgtahavrr gapsrasias enrsragssa
     1081 dteeslrerr hialtvncap pgppswtras sveerresir hadikqvtvv apdggpepsp
     1141 asspagrrtf kfsslkkras kaptasqdqp srttseadia afqkelqnlp nfetlagssd
     1201 afpeggaggl dgagyarprs csvprvtfea aslqlphghg plirsatteg aytgssaskp
     1261 aaaaappppt vsvpqpvqqs llsaceavap ahgplcelpg ahliilklly ewtcicpgdl
     1321 rirefrdfvn rlacmgdayk ewadelrria dlkeldkddv kpddleaihd eysklrdlvv
     1381 ngdllcskee aavlagvqlr ieetwpsnar trhvlgseql gklvpiteda keglipeppg
     1441 pgesssrmss aeaddspaes sdgagepatp tsrssgsflr vvpkrrslil rqwigsngnr
     1501 sptglhnavs lkeclppcyh taknigktik eqkrklfhsp lyeseihlkr lyiqtckrlp
     1561 sygchvfqvk ellrgktkkr ahrvlgigie hvvlldsktl vlaksqhtse lqqwragggr
     1621 shdrlvlefr gtkwsfvaaq slrsissvlw eimqdldarf ldehlittre eldkeshrnm
     1681 vsvhglerat iykeelerlq gllhfpeeva vrltdveyel fysvspvhyi rqvtvdlsgk
     1741 aatfnagekd rtvqalikrf nevsswvthi iisqpthedr kavlscilri amccwnignf
     1801 ntamevlagl kseklkpfwl slsdkdqlpv leflsqalln pepseeyraa veralniphs
     1861 kvipffgtfl rdlrailqgm pslvvlpsek akkiefvsdf hgedhfmtri gvggiinvek
     1921 ihqahrvldd ikafhhhykc rehalaqvai nskevrreet sckeddslyl qdpeayipiq
     1981 elandhhism vpltvdrvdf hqlqilhhgc tmvhweedgs rsslcyvrle rsnatitwck
     2041 pawsalrgsg pqdyalsvni eenvpvglsq kydtgesyla gleegfldln vvkeivlcrs
     2101 sievaavakr hgledsfsdq nllrfkfgag lsenrvlefi lpnflsviwy dslrrlvsml
     2161 kqqrqlcdgr ihwlketylq lyfreqscig ptpaeaikvf ggrkwtlnsl gssssvests
     2221 safkrassfg msttklrkkk sstslatvrd vgpkclpevd dggisarkqs ptikkrratv
     2281 ksppqlrsqe cspqrtsvsr sssscenlnr teggipivhp ttlatgyker scqvaastsk
     2341 assllsrpai thssqmnfle faelfrsfmv rcrkdlkdif esvatnskvt agelaaeeta
     2401 spksartrpk vlglltrssr feyvdpeqkk icdaiatasi vsncagvdss krlvlecadf
     2461 rkflatrqke dlteeevirl iqrhepdnvl reknllsfeg farylldkdn yafvpeiipp
     2521 gantnmdkpl shyyiasshn tyltghqlkg essvelysev llrgcrcvel dcwdgddgip
     2581 viyhghtlts kipfksvvea ihkssfatsp fpiilsienh cslpqqakma qiftnvfger
     2641 lqtqflfsnd vcdearlpsp nqlrykvlik nkklrppltp alplksakgv pqgkiapgrt
     2701 nsiistastg slndedeedy edydeeddnl eeslssqegs ckeamrhpgt lspgaahprp
     2761 rnpadaehlh sdddgykqrk sssqiapels dlvtycqaik frgfvtcpsp tnsvkvkkvs
     2821 skknvlasag splgvntplq tsepkhdtpt astasgsisa psstkrhcsn svcfqvssmn
     2881 esaakrlckr hplalithce nqlmrtypag mridssnfnp vtfwafglqm valnyqteda
     2941 glhintamfe qngscgyvlk psamwdknni mhgqynpwdk efdgihplnl hitvisgqyv
     3001 cqntyacspq vevevvgvpl dcsrqktkiv qrnslnpiwq dsfcfriafa elaflrfsvl
     3061 dvmtnhilsq rvipvkslkq gyrhvrlrsp qnqplplssl fvysefeeeg levvqqngdl
     3121 legaipkkak gkakelstae svrcdgrdnl vassqglkrr mfflvvhgvm pdepktilki
     3181 tedttsrevi aqalqkankp ndslddyvlm eetqknwdri tqeksttqri ldpdecplls
     3241 qttgantesr filkkladdp strawmttik sssnkerrka esgasgglse wsdgeetylv
     3301 cvynvsadqp yailkapiss taqdiiaqal vkarraedpi nfvlveqmey pspltesapa
     3361 sgssvrrrgl srgrrtlfdd envyevqaqw kargwfelre rneglserrk tsapksssfy
     3421 rlsrlrssmk skerdkscps pkqspdgrrs ggpstsaaaa ggagasarep ctsreaqepr
     3481 qvhsegeals dddappaama arlrkisfrk lkvwr
//
LOCUS       XP_077501809             454 aa            linear   INV 25-NOV-2025
DEFINITION  hexokinase A [Amblyomma americanum].
ACCESSION   XP_077501809
VERSION     XP_077501809.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645683.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..454
                     /product="hexokinase A"
                     /calculated_mol_wt=50837
     Region          18..435
                     /region_name="ASKHA_NBD_HK_meta"
                     /note="nucleotide-binding domain (NBD) of metazoan
                     hexokinase (HK) domain family; cd24019"
                     /db_xref="CDD:466869"
     CDS             1..454
                     /gene="Hex-A"
                     /coded_by="XM_077645683.1:174..1538"
                     /db_xref="GeneID:144112866"
ORIGIN      
        1 mmsekrkkve amtrdlelpn dvlqkvsakl ldefnkglgk ethksaevkm fvtyvrdvpn
       61 gseggtflal dlggtnfrvl lididgdrfs mqneiyaipq eimlgtgeel fdhiadclsk
      121 fmdkykvknk plplgftfsf pcrqegltva rlvhwtkgfk csgvenedvv hllrnaikkr
      181 kdididvmav vndttgtlms cahknkqcrl glivgtgtna cymeklenve lwdgdneepr
      241 qviintewga fgdhgslefv rtrydreids aslnpgkqlf ekmisgmymg elvrrvlvhl
      301 aeenlmfssk lsekmktpyl fktkyisqie seprgvydea hsvlvkmdmv gtdedceclk
      361 licsrvssra ahlvsaavat ilnkmkrpht tvgvdgsvyr fhprfhalme akiaelanpq
      421 ykfdlmlsed gsgrgaalva avavrtvkek aakk
//
LOCUS       XP_077501810             278 aa            linear   INV 25-NOV-2025
DEFINITION  BRISC complex subunit Abraxas 2-like [Amblyomma americanum].
ACCESSION   XP_077501810
VERSION     XP_077501810.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645684.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..278
                     /product="BRISC complex subunit Abraxas 2-like"
                     /calculated_mol_wt=30784
     Region          4..258
                     /region_name="Abraxas-like_domain"
                     /note="Abraxas-like domain of BRCA1-A complex subunit
                     Abraxas 1, BRISC complex subunit Abraxas 2, and similar
                     domains found in insects and plants; cd23519"
                     /db_xref="CDD:467802"
     Site            order(9,11,13..15,17..18,21..23,26,60..64,66,67..68,84,88,
                     98,100..106,108..111,113,139,144..151,166..167,170,
                     172..179,181..186,188,195..197,201,211..212,215..216,
                     218..219,222..223,225..227,229..230,233..234,236..241,
                     243..244,246..247,250..251,253..254,257)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:467802"
     Site            172
                     /site_type="active"
                     /note="BRCC36 activation site [active]"
                     /db_xref="CDD:467802"
     CDS             1..278
                     /gene="LOC144112867"
                     /coded_by="XM_077645684.1:217..1053"
                     /db_xref="GeneID:144112867"
ORIGIN      
        1 matvtvtisg pllatliyeh cnspgdqegf llgeitsrit dtisdaqlhg ekeetnlkic
       61 aiiscdlfef ydkkchlvtd kltallrdkq kevvgwyrfr rntslqaslr eqvlhqrlvr
      121 rlgqgygnyf llalfrgcts sndathsmeh vflrstdpaf khysavplri vnlgepghsd
      181 yrmypgsaas ihqgafcevl nslpadgsle mvdhmhrlhn llhtklrevl nsvaeseedv
      241 aafaddiiql rekygslkka vgpstsssas esisatsk
//
LOCUS       XP_077501811             385 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112868 [Amblyomma americanum].
ACCESSION   XP_077501811
VERSION     XP_077501811.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645685.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..385
                     /product="uncharacterized protein LOC144112868"
                     /calculated_mol_wt=42357
     CDS             1..385
                     /gene="LOC144112868"
                     /coded_by="XM_077645685.1:139..1296"
                     /db_xref="GeneID:144112868"
ORIGIN      
        1 mqfirrvlal fgmfrdvkhd qglpgwsmld hddtvvdicd fdehssssqh lfedvdsdav
       61 nastadssls gerkreqeqp tfavpaqlar qrfpvpvces ttpeeqksgt ldlapnltpt
      121 spqasascst gfdtdsrisc fvpdktqatg tcvrlprkrr kiyhlqgnqc aidessndds
      181 agdiyhksms vgarttidev tedelnssad taprslgmcn vgsqlpalen psqpvfkphd
      241 ihvgggespe qmqsaikngf eerlliarqr esseltlwqf ykenahfpkm lcpeaaealt
      301 arvltiesiy giaycacevd tacnvtskic ltlplelaes ekldcgavlr ifppwrehtl
      361 fsgekvlsav qylevlerpp petvd
//
LOCUS       XP_077501812             191 aa            linear   INV 25-NOV-2025
DEFINITION  adipose-secreted signaling protein isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501812
VERSION     XP_077501812.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645686.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..191
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..191
                     /product="adipose-secreted signaling protein isoform X1"
                     /calculated_mol_wt=21283
     Region          37..190
                     /region_name="DUF4517"
                     /note="Domain of unknown function (DUF4517); pfam15006"
                     /db_xref="CDD:464438"
     CDS             1..191
                     /gene="LOC144112869"
                     /coded_by="XM_077645686.1:154..729"
                     /db_xref="GeneID:144112869"
ORIGIN      
        1 meagqtkkap rlggivrrnl pgnneaemev dsrpsvhrvh fqesldtfgh dsgilvqpln
       61 dvlinahlgf iqinhryevr fqfdcpphlg slsvrtqdpp nlnlrvvelk pvvstglkye
      121 vvlellayke kilreqlvlq scnnplltlt lvlnarvlgk gkgtpflktg ihcigvemee
      181 esdlsdwqgf d
//
LOCUS       XP_077501813             191 aa            linear   INV 25-NOV-2025
DEFINITION  adipose-secreted signaling protein isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501813
VERSION     XP_077501813.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645687.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..191
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..191
                     /product="adipose-secreted signaling protein isoform X1"
                     /calculated_mol_wt=21283
     Region          37..190
                     /region_name="DUF4517"
                     /note="Domain of unknown function (DUF4517); pfam15006"
                     /db_xref="CDD:464438"
     CDS             1..191
                     /gene="LOC144112869"
                     /coded_by="XM_077645687.1:179..754"
                     /db_xref="GeneID:144112869"
ORIGIN      
        1 meagqtkkap rlggivrrnl pgnneaemev dsrpsvhrvh fqesldtfgh dsgilvqpln
       61 dvlinahlgf iqinhryevr fqfdcpphlg slsvrtqdpp nlnlrvvelk pvvstglkye
      121 vvlellayke kilreqlvlq scnnplltlt lvlnarvlgk gkgtpflktg ihcigvemee
      181 esdlsdwqgf d
//
LOCUS       XP_077501814             167 aa            linear   INV 25-NOV-2025
DEFINITION  adipose-secreted signaling protein isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501814
VERSION     XP_077501814.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645688.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..167
                     /product="adipose-secreted signaling protein isoform X2"
                     /calculated_mol_wt=18690
     Region          37..>158
                     /region_name="DUF4517"
                     /note="Domain of unknown function (DUF4517); pfam15006"
                     /db_xref="CDD:464438"
     CDS             1..167
                     /gene="LOC144112869"
                     /coded_by="XM_077645688.1:141..644"
                     /db_xref="GeneID:144112869"
ORIGIN      
        1 meagqtkkap rlggivrrnl pgnneaemev dsrpsvhrvh fqesldtfgh dsgilvqpln
       61 dvlinahlgf iqinhryevr fqfdcpphlg slsvrtqdpp nlnlrvvelk pvvstglkye
      121 vvlellayke kilreqlvlq scnnplltlt lvlnarvlvm cfsftai
//
LOCUS       XP_077501815             164 aa            linear   INV 25-NOV-2025
DEFINITION  adipose-secreted signaling protein isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501815
VERSION     XP_077501815.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645689.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..164
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..164
                     /product="adipose-secreted signaling protein isoform X3"
                     /calculated_mol_wt=18394
     Region          10..163
                     /region_name="DUF4517"
                     /note="Domain of unknown function (DUF4517); pfam15006"
                     /db_xref="CDD:464438"
     CDS             1..164
                     /gene="LOC144112869"
                     /coded_by="XM_077645689.1:186..680"
                     /db_xref="GeneID:144112869"
ORIGIN      
        1 mevdsrpsvh rvhfqesldt fghdsgilvq plndvlinah lgfiqinhry evrfqfdcpp
       61 hlgslsvrtq dppnlnlrvv elkpvvstgl kyevvlella ykekilreql vlqscnnpll
      121 tltlvlnarv lgkgkgtpfl ktgihcigve meeesdlsdw qgfd
//
LOCUS       XP_077501816             177 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112870 [Amblyomma americanum].
ACCESSION   XP_077501816
VERSION     XP_077501816.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645690.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..177
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..177
                     /product="uncharacterized protein LOC144112870"
                     /calculated_mol_wt=18740
     CDS             1..177
                     /gene="LOC144112870"
                     /coded_by="XM_077645690.1:173..706"
                     /db_xref="GeneID:144112870"
ORIGIN      
        1 msteagqqip grralmpgwm kshmdrlntv anlddmcssp petesgflrd mplsrltsah
       61 psqapsgppt irasgvskce lprlasedal rhqvlshhga slqnlgrgsg sgsesghttp
      121 svtpqsspsl vrrvveadlt avgvpmvdgk rlkpkkkppt rfavtnfdln avsptsw
//
LOCUS       XP_077501817             234 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112871 [Amblyomma americanum].
ACCESSION   XP_077501817
VERSION     XP_077501817.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645691.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..234
                     /product="uncharacterized protein LOC144112871"
                     /calculated_mol_wt=25863
     Region          58..191
                     /region_name="P-loop containing Nucleoside Triphosphate
                     Hydrolases"
                     /note="Members of the P-loop NTPase domain superfamily are
                     characterized by a conserved nucleotide phosphate-binding
                     motif, also referred to as the Walker A motif
                     (GxxxxGK[S/T], where x is any residue), and the Walker B
                     motif (hhhh[D/E], where h is a...; cl38936"
                     /db_xref="CDD:476819"
     Site            63..70
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206648"
     Site            89
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206648"
     Site            93..95
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206648"
     Site            109..112
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206648"
     Site            order(111..112,128..129)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206648"
     Site            168..171
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206648"
     CDS             1..234
                     /gene="LOC144112871"
                     /coded_by="XM_077645691.1:129..833"
                     /db_xref="GeneID:144112871"
ORIGIN      
        1 msvlfklgsi svprksflig scvivgvatc ftlryywkrr srkyyssvee tcadggkril
       61 vlglddsgks slllhlsrpd asstqpppte gfnvvcinse ngsslnfwel ggsetvrpyw
      121 pnflhdtsvl vymvnsadtk rlrsaahefr rilqderlkd vpilvlankq dlpeakpved
      181 isatlgldel kqlnfnvhil plqmpsdasc qstvaeakrr ilqlshgcsp ilal
//
LOCUS       XP_077501818             367 aa            linear   INV 25-NOV-2025
DEFINITION  guanylate binding protein 128up-like [Amblyomma americanum].
ACCESSION   XP_077501818
VERSION     XP_077501818.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645692.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..367
                     /product="guanylate binding protein 128up-like"
                     /calculated_mol_wt=40863
     Region          1..366
                     /region_name="Rbg1"
                     /note="Ribosome-interacting GTPase RBG1 [Translation,
                     ribosomal structure and biogenesis]; COG1163"
                     /db_xref="CDD:440777"
     CDS             1..367
                     /gene="LOC144112872"
                     /coded_by="XM_077645692.1:134..1237"
                     /db_xref="GeneID:144112872"
ORIGIN      
        1 mstvlekita ieaemartqr nkatmghlgl lkarlaklrr elitpkgggg gpgegfdvak
       61 tgdarigfvg fpsvgkstlm ntlagvfsev aayefttltt vpgvikykga kiqlldlpgi
      121 iegakdgkgr grqviavart cslifivldv lkplqhkril erevegfgir lnkeppaitl
      181 rkkdkgginl qmmvpqseld fdtvktilse ykihnaditl rcdatsddii dviegnriyi
      241 pciyvlnkid qisieeldii ykiphcvpis ahhkwnfddl lekawsylkl iriytkpkgq
      301 lpdyeapvil sqdrrtvedf cnklhrtimk efkcalvwgs svkhnpqkvg kehvlndedv
      361 vqvvkrv
//
LOCUS       XP_077501819             174 aa            linear   INV 25-NOV-2025
DEFINITION  U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein yantar
            isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501819
VERSION     XP_077501819.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645693.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..174
                     /product="U4/U6.U5 small nuclear ribonucleoprotein 27 kDa
                     protein yantar isoform X1"
                     /calculated_mol_wt=20738
     Region          118..173
                     /region_name="SNRNP27"
                     /note="U4/U6.U5 small nuclear ribonucleoproteins;
                     pfam08648"
                     /db_xref="CDD:462548"
     CDS             1..174
                     /gene="ytr"
                     /coded_by="XM_077645693.1:150..674"
                     /db_xref="GeneID:144112874"
ORIGIN      
        1 mgrsrsrspl rradrdrgrs rsrerdalrd pqrdrdldre rrlprerhrs rsrslsprlr
       61 nrsprrrrsr sprrrsrspr rrsrsrerke kvetpgalpk fllerppita kdlegkteee
      121 kemiklmgfa nfdsskgkhi pgnnvgavhv ihkrkyrqym nrkggfnrpl dfva
//
LOCUS       XP_077501820             173 aa            linear   INV 25-NOV-2025
DEFINITION  U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein yantar
            isoform X2 [Amblyomma americanum].
ACCESSION   XP_077501820
VERSION     XP_077501820.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645694.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..173
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..173
                     /product="U4/U6.U5 small nuclear ribonucleoprotein 27 kDa
                     protein yantar isoform X2"
                     /calculated_mol_wt=20667
     Region          117..172
                     /region_name="SNRNP27"
                     /note="U4/U6.U5 small nuclear ribonucleoproteins;
                     pfam08648"
                     /db_xref="CDD:462548"
     CDS             1..173
                     /gene="ytr"
                     /coded_by="XM_077645694.1:477..998"
                     /db_xref="GeneID:144112874"
ORIGIN      
        1 mgrsrsrspl rrdrdrgrsr srerdalrdp qrdrdldrer rlprerhrsr srslsprlrn
       61 rsprrrrsrs prrrsrsprr rsrsrerkek vetpgalpkf llerppitak dlegkteeek
      121 emiklmgfan fdsskgkhip gnnvgavhvi hkrkyrqymn rkggfnrpld fva
//
LOCUS       XP_077501821             144 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112875 [Amblyomma americanum].
ACCESSION   XP_077501821
VERSION     XP_077501821.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645695.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..144
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..144
                     /product="uncharacterized protein LOC144112875"
                     /calculated_mol_wt=15180
     CDS             1..144
                     /gene="LOC144112875"
                     /coded_by="XM_077645695.1:126..560"
                     /db_xref="GeneID:144112875"
ORIGIN      
        1 maegikggna rreatlaagp ylsvprtpfa acrprtptpv dvndlmekkq gtserhrstt
       61 nyqppssfgn aprprldatl ardlqsppwp pllpvtshrs ppadcapppa gphyhrnpgs
      121 spadqvcqgs ptaastikhr spgp
//
LOCUS       XP_077501822             546 aa            linear   INV 25-NOV-2025
DEFINITION  phosphoglucomutase 3-like protein nst [Amblyomma americanum].
ACCESSION   XP_077501822
VERSION     XP_077501822.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645696.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..546
                     /product="phosphoglucomutase 3-like protein nst"
                     /calculated_mol_wt=59698
     Region          26..527
                     /region_name="PGM3"
                     /note="PGM3 (phosphoglucomutase 3), also known as PAGM
                     (phosphoacetylglucosamine mutase) and AGM1
                     (N-acetylglucosamine-phosphate mutase), is an essential
                     enzyme found in eukaryotes that reversibly catalyzes the
                     conversion of GlcNAc-6-phosphate into...; cd03086"
                     /db_xref="CDD:100088"
     Site            order(32,67..68,278,280,282..283,353..355,372,374,376,500,
                     502..504,509)
                     /site_type="active"
                     /db_xref="CDD:100088"
     Site            order(278,280,282)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:100088"
     Site            order(353..355,372,374,376,500,502..504,509)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:100088"
     CDS             1..546
                     /gene="nst"
                     /coded_by="XM_077645696.1:56..1696"
                     /db_xref="GeneID:144112876"
ORIGIN      
        1 malffeqavk tafqnypkst vkplhygtag irdkgeilgs cmfrmgilaa lrskykkaiv
       61 gvmitashnp eddngvklid pmgemmetdw eilatelana adsslesvld rivaatninl
      121 qepsnvylay dtrvsspqla eavsdgiksi ggvvrtfghl ttpqlhyiir csndpaygep
      181 teegyfkklt kafmqirang savrnyvpfi rldgangvga mkikmlvphl anllkvelfn
      241 dgtegrlnhm cgadfvkvyq kapegipldv gvrclsfdgd adrviyfyhd enlvfhlldg
      301 dkiatlvasy lkelldaaml slnvvivqta yangsstnyi tnvlklpvkc vptgvkhlhr
      361 eaqkadigiy feanghgtvl fsekaqqaih dlandsnqys erqqaakkll ctmdlinqtv
      421 gdaisdmllv etvlhakgic gpewnslytd lpnrqlkvhv anrniitttn aerrclspsd
      481 lqpaidqlvq gchngrafvr psgtedivrv yaeassqdaa nklayevgmk vyelaggige
      541 kpklla
//
LOCUS       XP_077501823             198 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112877 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501823
VERSION     XP_077501823.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645697.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..198
                     /product="uncharacterized protein LOC144112877 isoform X1"
                     /calculated_mol_wt=21214
     CDS             1..198
                     /gene="LOC144112877"
                     /coded_by="XM_077645697.1:247..843"
                     /db_xref="GeneID:144112877"
ORIGIN      
        1 mrrvlvsttr ststiaaakq ekkavtgirr vfvssiinrt maatcswfsg hgkskktgas
       61 efkvekamyd tvkgipmdav fhkrtaipiw steavvlcss sgkcsggdsg rqgkgivatq
      121 lesscyqgns tystlpekth ammlgasilt ilpilstnea kdafvqiaes qqppwkatte
      181 wlrsgwgret gmvvgkfg
//
LOCUS       XP_077501824             186 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112877 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501824
VERSION     XP_077501824.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645698.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..186
                     /product="uncharacterized protein LOC144112877 isoform X2"
                     /calculated_mol_wt=19818
     CDS             1..186
                     /gene="LOC144112877"
                     /coded_by="XM_077645698.1:403..963"
                     /db_xref="GeneID:144112877"
ORIGIN      
        1 msplsrfgav lvcacsayia ehniinrtma atcswfsghg kskktgasef kvekamydtv
       61 kgipmdavfh krtaipiwst eavvlcsssg kcsggdsgrq gkgivatqle sscyqgnsty
      121 stlpektham mlgasiltil pilstneakd afvqiaesqq ppwkattewl rsgwgretgm
      181 vvgkfg
//
LOCUS       XP_077501825             154 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112877 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501825
VERSION     XP_077501825.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645699.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..154
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..154
                     /product="uncharacterized protein LOC144112877 isoform X3"
                     /calculated_mol_wt=16756
     CDS             1..154
                     /gene="LOC144112877"
                     /coded_by="XM_077645699.1:210..674"
                     /db_xref="GeneID:144112877"
ORIGIN      
        1 mrrvlvsttr ststiaaakq ekkavtgirr vfvssiinrt maatcswfsg hgkskktgas
       61 efkvekamyd tvkgipmdav fhkrtaipiw steavvlcss sgkcsggdsg rhcgytagvk
      121 llsgkfnlfd farkdaryda wglhldytan fvyq
//
LOCUS       XP_077501826             198 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112878 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501826
VERSION     XP_077501826.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645700.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..198
                     /product="uncharacterized protein LOC144112878 isoform X1"
                     /calculated_mol_wt=21214
     CDS             1..198
                     /gene="LOC144112878"
                     /coded_by="XM_077645700.1:247..843"
                     /db_xref="GeneID:144112878"
ORIGIN      
        1 mrrvlvsttr ststiaaakq ekkavtgirr vfvssiinrt maatcswfsg hgkskktgas
       61 efkvekamyd tvkgipmdav fhkrtaipiw steavvlcss sgkcsggdsg rqgkgivatq
      121 lesscyqgns tystlpekth ammlgasilt ilpilstnea kdafvqiaes qqppwkatte
      181 wlrsgwgret gmvvgkfg
//
LOCUS       XP_077501827             186 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112878 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501827
VERSION     XP_077501827.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645701.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..186
                     /product="uncharacterized protein LOC144112878 isoform X2"
                     /calculated_mol_wt=19818
     CDS             1..186
                     /gene="LOC144112878"
                     /coded_by="XM_077645701.1:403..963"
                     /db_xref="GeneID:144112878"
ORIGIN      
        1 msplsrfgav lvcacsayia ehniinrtma atcswfsghg kskktgasef kvekamydtv
       61 kgipmdavfh krtaipiwst eavvlcsssg kcsggdsgrq gkgivatqle sscyqgnsty
      121 stlpektham mlgasiltil pilstneakd afvqiaesqq ppwkattewl rsgwgretgm
      181 vvgkfg
//
LOCUS       XP_077501828             154 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112878 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501828
VERSION     XP_077501828.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645702.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..154
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..154
                     /product="uncharacterized protein LOC144112878 isoform X3"
                     /calculated_mol_wt=16756
     CDS             1..154
                     /gene="LOC144112878"
                     /coded_by="XM_077645702.1:210..674"
                     /db_xref="GeneID:144112878"
ORIGIN      
        1 mrrvlvsttr ststiaaakq ekkavtgirr vfvssiinrt maatcswfsg hgkskktgas
       61 efkvekamyd tvkgipmdav fhkrtaipiw steavvlcss sgkcsggdsg rhcgytagvk
      121 llsgkfnlfd farkdaryda wglhldytan fvyq
//
LOCUS       XP_077501829             325 aa            linear   INV 25-NOV-2025
DEFINITION  sulfotransferase ssu-1-like [Amblyomma americanum].
ACCESSION   XP_077501829
VERSION     XP_077501829.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645703.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..325
                     /product="sulfotransferase ssu-1-like"
                     /calculated_mol_wt=37517
     Region          38..306
                     /region_name="Sulfotransfer_1"
                     /note="Sulfotransferase domain; pfam00685"
                     /db_xref="CDD:425820"
     CDS             1..325
                     /gene="LOC144112880"
                     /coded_by="XM_077645703.1:104..1081"
                     /db_xref="GeneID:144112880"
ORIGIN      
        1 masrrlpkyv dieglrltse fspepvreal kfvpragdvv lvtypkcgth waqqilqliv
       61 nggrsadnfy ewlartplle llgtriledr peprlmkthl plarlalrde aryvyvarnp
      121 wdccvsfyhh tktlpaadfr egtfddffel fvsgqtdhgg ffdhllpwya lrtqpnvffi
      181 tyeqlakdkr atvlkiacfl gphyeqellh nkerllavld kssadfmkrq faldpailks
      241 ildrdpnalp demktlfgkv fvaperetnt vnfvrkgivg dsknyftreq lqrmqqlidk
      301 ktagtdvmhm wldedplrql etesp
//
LOCUS       XP_077501830             325 aa            linear   INV 25-NOV-2025
DEFINITION  sulfotransferase ssu-1-like [Amblyomma americanum].
ACCESSION   XP_077501830
VERSION     XP_077501830.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645704.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..325
                     /product="sulfotransferase ssu-1-like"
                     /calculated_mol_wt=37517
     Region          38..306
                     /region_name="Sulfotransfer_1"
                     /note="Sulfotransferase domain; pfam00685"
                     /db_xref="CDD:425820"
     CDS             1..325
                     /gene="LOC144112880"
                     /coded_by="XM_077645704.1:129..1106"
                     /db_xref="GeneID:144112880"
ORIGIN      
        1 masrrlpkyv dieglrltse fspepvreal kfvpragdvv lvtypkcgth waqqilqliv
       61 nggrsadnfy ewlartplle llgtriledr peprlmkthl plarlalrde aryvyvarnp
      121 wdccvsfyhh tktlpaadfr egtfddffel fvsgqtdhgg ffdhllpwya lrtqpnvffi
      181 tyeqlakdkr atvlkiacfl gphyeqellh nkerllavld kssadfmkrq faldpailks
      241 ildrdpnalp demktlfgkv fvaperetnt vnfvrkgivg dsknyftreq lqrmqqlidk
      301 ktagtdvmhm wldedplrql etesp
//
LOCUS       XP_077501831             173 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112881 [Amblyomma americanum].
ACCESSION   XP_077501831
VERSION     XP_077501831.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645705.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..173
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..173
                     /product="uncharacterized protein LOC144112881"
                     /calculated_mol_wt=19279
     CDS             1..173
                     /gene="LOC144112881"
                     /coded_by="XM_077645705.1:297..818"
                     /db_xref="GeneID:144112881"
ORIGIN      
        1 mgpsnkalwk icgiaatrsv kpvvscrhpf aantdrqlyf fadaphilkn irghlvrgqs
       61 fflpaaivtk yelptdevsv khirelavid aerdlklaph lkpahldpdh fakmnvasav
      121 avlnhsvgaa mrtlvslrll esealttawf vervfrwftr mtsryiatam slf
//
LOCUS       XP_077501832             790 aa            linear   INV 25-NOV-2025
DEFINITION  fibroblast growth factor receptor 4-like [Amblyomma americanum].
ACCESSION   XP_077501832
VERSION     XP_077501832.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645706.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..790
                     /product="fibroblast growth factor receptor 4-like"
                     /calculated_mol_wt=88601
     Region          34..112
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          43..47
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          56..60
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          92..97
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          152..228
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          258..351
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          267..271
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          280..284
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          317..321
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          331..336
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          344..347
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          461..753
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(480..483,486,488,506,508,539,555..558,617,621..622,
                     624,634..635)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..790
                     /gene="LOC144112883"
                     /coded_by="XM_077645706.1:58..2430"
                     /db_xref="GeneID:144112883"
ORIGIN      
        1 mcrfrlgvlt fislfcfass egppvikkdn ektvyevmpg dtvrlrcaas gqprphvawy
       61 hgesevtpar dnvrqsrhal viqnaqprda gryfckasnd dgvawknftv lvevvsadqa
      121 rgrdnilhtr nasavtipsi detedlteka epptlakvnp hlvvqlagsn lvlpcivksn
      181 ppanitwlkn dqrptrllgk kityrkqklr mndvmvsdag nytciavnsl gnvrytyrve
      241 ikeriplrpt fyedffenkt lyvgqqaqle crfisdlqph vswlrhysvn gswfqddlet
      301 tpyvsivqsa nestdprilv ienvtlddeg wytclvgnsv gissksgyls vkplpqpeks
      361 rfaltllkhp wmlalaivlv vsavvavati lirrsamlsl qqkkldlaqk qhmvvrkkvi
      421 lerqisdsgh yedsfiaplv kidhqsgflp selntvceye lpldpnwefp rerlilgkhl
      481 gegafgqvvk aeahglgdke gptivavkml kdghtdsema dlvsemevmk tigkhiniin
      541 llgcctqdgp lhviveyaah gnlrdflrtr rpssgyeeai gadlkpktlt hkdlvsfayq
      601 vargmeylas kkcihrdlaa rnvlvvennv mkiadfglar dlhnidyykk ksggllpvkw
      661 mapeslfdrv ytpqsdvwsy gillweimtl ggtpypsvpi eklfqllrdg hrmekpqgcs
      721 levyvimkec whqspyqrpa ftklvedldr iltlatdqey ldlsipvldt ppsssesvss
      781 lsfaaphvav
//
LOCUS       XP_077501833             255 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112884 [Amblyomma americanum].
ACCESSION   XP_077501833
VERSION     XP_077501833.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645707.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..255
                     /product="uncharacterized protein LOC144112884"
                     /calculated_mol_wt=28532
     CDS             1..255
                     /gene="LOC144112884"
                     /coded_by="XM_077645707.1:1..768"
                     /db_xref="GeneID:144112884"
ORIGIN      
        1 mqvstsyalf akkssnyilv qfpsphccva iavecahvih sllllsgdve tnpgpeenaa
       61 vlaelqklnv gqtlliteiq glktqlnttd qtiasldkrm adleahyqtl lhlrndieim
      121 qsttinqakk ieeletrldg aenqsrrknl ifygipdpas aetwaesekl vidvcrnnld
      181 itlepndier ahrlgnhsad rtrpvivkfl shktkdalls ngrklkdtny sigedfsrtv
      241 rharkqllvf cqsqf
//
LOCUS       XP_077501834             116 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112886 [Amblyomma americanum].
ACCESSION   XP_077501834
VERSION     XP_077501834.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645708.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..116
                     /product="uncharacterized protein LOC144112886"
                     /calculated_mol_wt=13016
     CDS             1..116
                     /gene="LOC144112886"
                     /coded_by="XM_077645708.1:92..442"
                     /db_xref="GeneID:144112886"
ORIGIN      
        1 mestttllhe eqcpvdeypv akevmraiqn tdpmcfgalt sgpqfveqkr spqeicegrr
       61 rvqpdrekrr irvpvpdiap tvqlwwhtlp laaqflpvgp llssarpwgh stskst
//
LOCUS       XP_077501835             113 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112887 [Amblyomma americanum].
ACCESSION   XP_077501835
VERSION     XP_077501835.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645709.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..113
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..113
                     /product="uncharacterized protein LOC144112887"
                     /calculated_mol_wt=11601
     CDS             1..113
                     /gene="LOC144112887"
                     /coded_by="XM_077645709.1:98..439"
                     /db_xref="GeneID:144112887"
ORIGIN      
        1 mrgrssasqs ssggassmde hssggrwqep gsspvygsah tddapvdrpa rdgssrlana
       61 rtvvciakvc dnqgvlepva ipatylhrar grprpdlyyg hgpgvpgpvd sca
//
LOCUS       XP_077501836              96 aa            linear   INV 25-NOV-2025
DEFINITION  importin-7-like [Amblyomma americanum].
ACCESSION   XP_077501836
VERSION     XP_077501836.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645710.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..96
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..96
                     /product="importin-7-like"
                     /calculated_mol_wt=10419
     CDS             1..96
                     /gene="LOC144112888"
                     /coded_by="XM_077645710.1:42..332"
                     /db_xref="GeneID:144112888"
ORIGIN      
        1 mestttslhe ehcpvdeypv akevmraiqn tdpmcfgalt sgpqfveqkr spqeicaltd
       61 qtkaaaesnr ieksggyvfq nqtlpppfss ggtpfr
//
LOCUS       XP_077501837              96 aa            linear   INV 25-NOV-2025
DEFINITION  importin-7-like [Amblyomma americanum].
ACCESSION   XP_077501837
VERSION     XP_077501837.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645711.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..96
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..96
                     /product="importin-7-like"
                     /calculated_mol_wt=10526
     CDS             1..96
                     /gene="LOC144112889"
                     /coded_by="XM_077645711.1:108..398"
                     /db_xref="GeneID:144112889"
ORIGIN      
        1 mesttmslhe eqcpvdeypv akevmraiqn tdpmcfgalt sgpqfveqkr sqqeicvltd
       61 qtkaaaesnr ieksggyvfq nqtlpppfss ggkpfr
//
LOCUS       XP_077501838              96 aa            linear   INV 25-NOV-2025
DEFINITION  importin-7-like [Amblyomma americanum].
ACCESSION   XP_077501838
VERSION     XP_077501838.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645712.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..96
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..96
                     /product="importin-7-like"
                     /calculated_mol_wt=10484
     CDS             1..96
                     /gene="LOC144112890"
                     /coded_by="XM_077645712.1:96..386"
                     /db_xref="GeneID:144112890"
ORIGIN      
        1 mesttmslhe eqcpvdeypg akevmraiqn tdpmcfgalt sgpqfveqkr sqqeicvltd
       61 qtkaaaesnr ieksggyvfq nqtlpppfss ggkpfr
//
LOCUS       XP_077501839              96 aa            linear   INV 25-NOV-2025
DEFINITION  importin-7-like [Amblyomma americanum].
ACCESSION   XP_077501839
VERSION     XP_077501839.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645713.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..96
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..96
                     /product="importin-7-like"
                     /calculated_mol_wt=10453
     CDS             1..96
                     /gene="LOC144112891"
                     /coded_by="XM_077645713.1:96..386"
                     /db_xref="GeneID:144112891"
ORIGIN      
        1 mesttmslhe eqcpvdeypg akevmraiqn tdpmcfgalt sgpqfveqkr spqeicvltd
       61 qtkaaaesnr ieksggyvfq nqtlpppfss ggkpfr
//
LOCUS       XP_077501840              96 aa            linear   INV 25-NOV-2025
DEFINITION  importin-7-like [Amblyomma americanum].
ACCESSION   XP_077501840
VERSION     XP_077501840.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645714.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..96
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..96
                     /product="importin-7-like"
                     /calculated_mol_wt=10499
     CDS             1..96
                     /gene="LOC144112892"
                     /coded_by="XM_077645714.1:93..383"
                     /db_xref="GeneID:144112892"
ORIGIN      
        1 mesttmslhe eqcpvdeypv akevmraiqn tdpmcfgalt sgpqfveqkr sqqeicvltd
       61 qtkaaaesnr ieksggyvfq nqtlpppfss ggtpfr
//
LOCUS       XP_077501841              96 aa            linear   INV 25-NOV-2025
DEFINITION  importin-7-like [Amblyomma americanum].
ACCESSION   XP_077501841
VERSION     XP_077501841.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645715.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..96
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..96
                     /product="importin-7-like"
                     /calculated_mol_wt=10437
     CDS             1..96
                     /gene="LOC144112893"
                     /coded_by="XM_077645715.1:108..398"
                     /db_xref="GeneID:144112893"
ORIGIN      
        1 mestttslhe eqcpvdeypv akevmraiqn tdpmcfgalt sgpqfveqkr spqkicvltd
       61 qtkaaaesnr ieksggyvfq nqtlpppfss ggtpfr
//
LOCUS       XP_077501842             785 aa            linear   INV 25-NOV-2025
DEFINITION  vacuolar protein sorting 51 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501842
VERSION     XP_077501842.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645716.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..785
                     /product="vacuolar protein sorting 51 isoform X1"
                     /calculated_mol_wt=89384
     Region          42..127
                     /region_name="Vps51"
                     /note="Vps51/Vps67; pfam08700"
                     /db_xref="CDD:462568"
     Region          57..>223
                     /region_name="COG2"
                     /note="COG (conserved oligomeric Golgi) complex component,
                     COG2; cl19297"
                     /db_xref="CDD:473160"
     CDS             1..785
                     /gene="Vps51"
                     /coded_by="XM_077645716.1:140..2497"
                     /db_xref="GeneID:144112894"
ORIGIN      
        1 makelsgdek emlrkknqrk mlkqfyhgsa esadagfnpy dingsdfnpd lylnkllkec
       61 tltdlmdkeq eiykqiqald gemqtlvyen ynkfisatdt irkmkndfkr meeemdhlss
      121 nmatiakfss siagtlqgrr eqmiklssth sllkklqllf elpprlkaci esesyeeavt
      181 yytkaqkvlk qykhmpsfsg ihddcaaive vlrerlrkkf hepevtsrel sgvvellfql
      241 feptstlctd yllnarrpld rdlkelqrqv qmlmdpaslg aterkgkdav ldlpmhilef
      301 tnmacnciit glcniiesyn dlfinrlpme yyengetdav vvqklkafld eimlqllgvv
      361 errvaaetrr vdssvlvkal drfcqklqal tkmmpaadys qqaahiveqa ardqcafalq
      421 slgqffaerl tdvrhnlalp rtvgqdslsl adlhaslhsl ildqmqsllr glrafvqaev
      481 efskranfqd mfclevhegi vvafirtink kalefcetag eksggappsl llllsrfsve
      541 mkntlvsrll amadehfgip daknltpasq liqetsdvaq tlidqfvrvq gllisqmlrk
      601 sveardwmna leprnvravm krvvedvtav dvrvgqlyee garnerssds srrtypysas
      661 grahwsarwp ssvdhsllsn iqklfsekid ifttvefsrv svmtgiikig lktflecvrl
      721 ktfsryglqq vqvdthylql ylwrfvsden lvhvlldelm gsaihrcfdp mlmepsvvev
      781 icerg
//
LOCUS       XP_077501843             774 aa            linear   INV 25-NOV-2025
DEFINITION  vacuolar protein sorting 51 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077501843
VERSION     XP_077501843.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645717.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..774
                     /product="vacuolar protein sorting 51 isoform X2"
                     /calculated_mol_wt=88166
     Region          31..116
                     /region_name="Vps51"
                     /note="Vps51/Vps67; pfam08700"
                     /db_xref="CDD:462568"
     Region          46..>212
                     /region_name="COG2"
                     /note="COG (conserved oligomeric Golgi) complex component,
                     COG2; cl19297"
                     /db_xref="CDD:473160"
     CDS             1..774
                     /gene="Vps51"
                     /coded_by="XM_077645717.1:277..2601"
                     /db_xref="GeneID:144112894"
ORIGIN      
        1 mlrkknqrkm lkqfyhgsae sadagfnpyd ingsdfnpdl ylnkllkect ltdlmdkeqe
       61 iykqiqaldg emqtlvyeny nkfisatdti rkmkndfkrm eeemdhlssn matiakfsss
      121 iagtlqgrre qmiklssths llkklqllfe lpprlkacie sesyeeavty ytkaqkvlkq
      181 ykhmpsfsgi hddcaaivev lrerlrkkfh epevtsrels gvvellfqlf eptstlctdy
      241 llnarrpldr dlkelqrqvq mlmdpaslga terkgkdavl dlpmhileft nmacnciitg
      301 lcniiesynd lfinrlpmey yengetdavv vqklkaflde imlqllgvve rrvaaetrrv
      361 dssvlvkald rfcqklqalt kmmpaadysq qaahiveqaa rdqcafalqs lgqffaerlt
      421 dvrhnlalpr tvgqdslsla dlhaslhsli ldqmqsllrg lrafvqaeve fskranfqdm
      481 fclevhegiv vafirtinkk alefcetage ksggappsll lllsrfsvem kntlvsrlla
      541 madehfgipd aknltpasql iqetsdvaqt lidqfvrvqg llisqmlrks veardwmnal
      601 eprnvravmk rvvedvtavd vrvgqlyeeg arnerssdss rrtypysasg rahwsarwps
      661 svdhsllsni qklfsekidi fttvefsrvs vmtgiikigl ktflecvrlk tfsryglqqv
      721 qvdthylqly lwrfvsdenl vhvlldelmg saihrcfdpm lmepsvvevi cerg
//
LOCUS       XP_077501844             105 aa            linear   INV 25-NOV-2025
DEFINITION  thioredoxin-dependent peroxide reductase, mitochondrial-like
            [Amblyomma americanum].
ACCESSION   XP_077501844
VERSION     XP_077501844.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645718.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..105
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..105
                     /product="thioredoxin-dependent peroxide reductase,
                     mitochondrial-like"
                     /calculated_mol_wt=11449
     Region          <3..87
                     /region_name="Thioredoxin_like"
                     /note="Protein Disulfide Oxidoreductases and Other
                     Proteins with a Thioredoxin fold; cl00388"
                     /db_xref="CDD:469754"
     CDS             1..105
                     /gene="LOC144112899"
                     /coded_by="XM_077645718.1:2916..3233"
                     /db_xref="GeneID:144112899"
ORIGIN      
        1 mlgglgkvnv tllsdftkki skdynvlled igialrasfi idpkgmvrqv tvndvnlyrs
       61 vdetlrllka lqhvekhgaa icaswepepa ggrgcersar srqdk
//
LOCUS       XP_077501845             105 aa            linear   INV 25-NOV-2025
DEFINITION  thioredoxin-dependent peroxide reductase, mitochondrial-like
            [Amblyomma americanum].
ACCESSION   XP_077501845
VERSION     XP_077501845.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645719.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..105
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..105
                     /product="thioredoxin-dependent peroxide reductase,
                     mitochondrial-like"
                     /calculated_mol_wt=11449
     Region          <3..87
                     /region_name="Thioredoxin_like"
                     /note="Protein Disulfide Oxidoreductases and Other
                     Proteins with a Thioredoxin fold; cl00388"
                     /db_xref="CDD:469754"
     CDS             1..105
                     /gene="LOC144112899"
                     /coded_by="XM_077645719.1:2709..3026"
                     /db_xref="GeneID:144112899"
ORIGIN      
        1 mlgglgkvnv tllsdftkki skdynvlled igialrasfi idpkgmvrqv tvndvnlyrs
       61 vdetlrllka lqhvekhgaa icaswepepa ggrgcersar srqdk
//
LOCUS       XP_077501846             105 aa            linear   INV 25-NOV-2025
DEFINITION  thioredoxin-dependent peroxide reductase, mitochondrial-like
            [Amblyomma americanum].
ACCESSION   XP_077501846
VERSION     XP_077501846.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645720.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..105
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..105
                     /product="thioredoxin-dependent peroxide reductase,
                     mitochondrial-like"
                     /calculated_mol_wt=11449
     Region          <3..87
                     /region_name="Thioredoxin_like"
                     /note="Protein Disulfide Oxidoreductases and Other
                     Proteins with a Thioredoxin fold; cl00388"
                     /db_xref="CDD:469754"
     CDS             1..105
                     /gene="LOC144112899"
                     /coded_by="XM_077645720.1:2401..2718"
                     /db_xref="GeneID:144112899"
ORIGIN      
        1 mlgglgkvnv tllsdftkki skdynvlled igialrasfi idpkgmvrqv tvndvnlyrs
       61 vdetlrllka lqhvekhgaa icaswepepa ggrgcersar srqdk
//
LOCUS       XP_077501847             105 aa            linear   INV 25-NOV-2025
DEFINITION  thioredoxin-dependent peroxide reductase, mitochondrial-like
            [Amblyomma americanum].
ACCESSION   XP_077501847
VERSION     XP_077501847.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645721.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..105
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..105
                     /product="thioredoxin-dependent peroxide reductase,
                     mitochondrial-like"
                     /calculated_mol_wt=11449
     Region          <3..87
                     /region_name="Thioredoxin_like"
                     /note="Protein Disulfide Oxidoreductases and Other
                     Proteins with a Thioredoxin fold; cl00388"
                     /db_xref="CDD:469754"
     CDS             1..105
                     /gene="LOC144112899"
                     /coded_by="XM_077645721.1:2271..2588"
                     /db_xref="GeneID:144112899"
ORIGIN      
        1 mlgglgkvnv tllsdftkki skdynvlled igialrasfi idpkgmvrqv tvndvnlyrs
       61 vdetlrllka lqhvekhgaa icaswepepa ggrgcersar srqdk
//
LOCUS       XP_077501848             105 aa            linear   INV 25-NOV-2025
DEFINITION  thioredoxin-dependent peroxide reductase, mitochondrial-like
            [Amblyomma americanum].
ACCESSION   XP_077501848
VERSION     XP_077501848.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645722.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..105
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..105
                     /product="thioredoxin-dependent peroxide reductase,
                     mitochondrial-like"
                     /calculated_mol_wt=11449
     Region          <3..87
                     /region_name="Thioredoxin_like"
                     /note="Protein Disulfide Oxidoreductases and Other
                     Proteins with a Thioredoxin fold; cl00388"
                     /db_xref="CDD:469754"
     CDS             1..105
                     /gene="LOC144112899"
                     /coded_by="XM_077645722.1:2193..2510"
                     /db_xref="GeneID:144112899"
ORIGIN      
        1 mlgglgkvnv tllsdftkki skdynvlled igialrasfi idpkgmvrqv tvndvnlyrs
       61 vdetlrllka lqhvekhgaa icaswepepa ggrgcersar srqdk
//
LOCUS       XP_077501849             260 aa            linear   INV 25-NOV-2025
DEFINITION  receptor expression-enhancing protein 1-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501849
VERSION     XP_077501849.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645723.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..260
                     /product="receptor expression-enhancing protein 1-like
                     isoform X1"
                     /calculated_mol_wt=29183
     Region          10..86
                     /region_name="TB2_DP1_HVA22"
                     /note="TB2/DP1, HVA22 family; pfam03134"
                     /db_xref="CDD:460820"
     CDS             1..260
                     /gene="LOC144112906"
                     /coded_by="XM_077645723.1:128..910"
                     /db_xref="GeneID:144112906"
ORIGIN      
        1 msllfgvlyp ayasykavkn kdvrchlqwm mywivfalft cvetfsdifi sfwmpfyyel
       61 kilfvlwlls patkgsslly rrfvhpqlvq reqeidelie kakeqgystm lllgsrglsy
      121 atsvvlqtai kaqaaiiahr spelpalrdr qdqsdgnaia wmdvesdpep ppllpargqr
      181 ppplmgtgdn ldidnnvysp npgvhgeeic skshststkv srssvkhrtr atgktadssg
      241 qvrrstratk srsnkdlknl
//
LOCUS       XP_077501850             269 aa            linear   INV 25-NOV-2025
DEFINITION  receptor expression-enhancing protein 1-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501850
VERSION     XP_077501850.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645724.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..269
                     /product="receptor expression-enhancing protein 1-like
                     isoform X2"
                     /calculated_mol_wt=30204
     Region          19..95
                     /region_name="TB2_DP1_HVA22"
                     /note="TB2/DP1, HVA22 family; pfam03134"
                     /db_xref="CDD:460820"
     CDS             1..269
                     /gene="LOC144112906"
                     /coded_by="XM_077645724.1:191..1000"
                     /db_xref="GeneID:144112906"
ORIGIN      
        1 misivisrli illfgvlypa yasykavknk dvrchlqwmm ywivfalftc vetfsdifis
       61 fwmpfyyelk ilfvlwllsp atkgssllyr rfvhpqlvqr eqeideliek akeqgystml
      121 llgsrglsya tsvvlqtaik aqaaiiahrs pelpalrdrq dqsdgnaiaw mdvesdpepp
      181 pllpargqrp pplmgtgdnl didnnvyspn pgvhgeeics kshststkvs rssvkhrtra
      241 tgktadssgq vrrstratks rsnkdlknl
//
LOCUS       XP_077501851            1326 aa            linear   INV 25-NOV-2025
DEFINITION  RRP12-like protein [Amblyomma americanum].
ACCESSION   XP_077501851
VERSION     XP_077501851.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645725.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (DAPXPI010024505.1) annotated
            using gene prediction method: Gnomon, supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 1455 transcript bases to patch genome
                            assembly gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..1326
                     /product="RRP12-like protein"
                     /calculated_mol_wt=147732
     Region          516..715
                     /region_name="NUC173"
                     /note="NUC173 domain; pfam08161"
                     /db_xref="CDD:462386"
     CDS             1..1326
                     /gene="LOC144112908"
                     /coded_by="XM_077645725.1:1..3981"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 1455 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144112908"
ORIGIN      
        1 mdtdegpeva ersqgfllce edskhfippg dqesprhvrg fpfmaegfpn kgrlletgfk
       61 ciqdkggdvq nsvevmhfec edrpyatgdp gkggktltie qlqqhnahmn evgveddild
      121 rlsvagtetn rtadtdwtfa samtmnklvn kwdptsplhk emlamynatt evikasggqq
      181 tdteyfgvlm ttletvdtdi srtsaaalla ifanripaav lrkkfswitt lcinfmqmyq
      241 hplhimllrs liclmasclk slelaawses stlqvfsclf pyivhtrpkl rklaqkavvh
      301 vltgshamaa tgappthpav pavvafclpv irsssamdvy stslpvttlh mfgffkstln
      361 lfpqsevkst ceamlevmga ghptvvrcgl ltlqsffssq prhdvtlsae lnaqlinaly
      421 dykpgqrdhk lydvwlgtiq ealinlqrss delflsnlpk fvgvlvdswq sgkpelnyii
      481 scvlmvllqk cfqnclekes dptsksslcy tagvkviehm qralhyqflp awdqvlfawa
      541 tcfevlgkrc adhmleplsy iaelhgsqty tndaivdrai aaairtmgpe vvlkavpldm
      601 sgeescldfk rswmlpllrd nikatrlslf ityflplasa fhelaegsdk ptsvtktyei
      661 lekqiwsllp gfctrptdfk esfpriartl gtcllnrpyl ridimsalrh iincnfvnea
      721 nvpemtrysk nflpilfniy tseatssgae gvrlaayeti kpfvrvaddk lcsalffsas
      781 arllsgeist hakhavldla rslvrkmape nvqrlyllgk anledsdrrv qkkayrvlee
      841 icrfptptck vfvekhltel sevlveslqs itpgsraprl rcllllignl svqhksfalt
      901 alreavlcik vnaskvrqaa yeiviavgka lirwqpedpd aavkelvlql tagftggsyl
      961 vsctilalat alyefkgtys kaimnavmea vqivisspnr evvqaalyfv rmlfvilste
     1021 elsqhlqtlv pnlccmsedc rkhfrlklrd ifaklirkfg yenivglvpe syrklavrvr
     1081 kleqrkkrkk karelqvmqe dnvvssavpg kacvegieqi lqsssedese kvcdksqgrk
     1141 kttvaarawi eeqgeddivd fldtsagkqi lstdpkaapk segkcpfeit kdgrllivdp
     1201 esakaqkkge pasdteeaan nllealshyk tkkrkmpqda ddgvetwgtg sgagpskkrr
     1261 tekasdgdar kgklepytyv plsrtslnkr fkrkpaefkr llvavqkgaq kgqkkraanl
     1321 kkrssq
//
LOCUS       XP_077501852             221 aa            linear   INV 25-NOV-2025
DEFINITION  vacuolar protein sorting 24 [Amblyomma americanum].
ACCESSION   XP_077501852
VERSION     XP_077501852.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645726.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..221
                     /product="vacuolar protein sorting 24"
                     /calculated_mol_wt=24792
     Region          18..186
                     /region_name="Snf7"
                     /note="pfam03357"
                     /db_xref="CDD:460896"
     CDS             1..221
                     /gene="Vps24"
                     /coded_by="XM_077645726.1:125..790"
                     /db_xref="GeneID:144112909"
ORIGIN      
        1 mglfgktper spkeqvrewt sklrkegyql drqiraiqrq eegvkkslke aakkndkevc
       61 lilakevlra rkaisrihas kaqlnsvvms mnhqlatlrl agsmqrstev mksmqqlikv
      121 pevaqtmrdl skemmragii eemlddtmeg lddqedleee aqeevdkvlw eltagqlgka
      181 psavteslps edvqeagpss alaededdvt kmqerlqalr s
//
LOCUS       XP_077501853             790 aa            linear   INV 25-NOV-2025
DEFINITION  tudor domain-containing protein 1-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501853
VERSION     XP_077501853.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645727.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..790
                     /product="tudor domain-containing protein 1-like isoform
                     X1"
                     /calculated_mol_wt=88875
     Region          <74..145
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          <344..377
                     /region_name="Tudor_dTUD-like"
                     /note="Tudor domain found in Drosophila melanogaster
                     maternal protein Tudor (dTUD) and similar proteins;
                     cd20379"
                     /db_xref="CDD:410450"
     Region          514..539
                     /region_name="zf-CCCH"
                     /note="Zinc finger C-x8-C-x5-C-x3-H type (and similar);
                     pfam00642"
                     /db_xref="CDD:459885"
     Region          575..704
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     CDS             1..790
                     /gene="LOC144112910"
                     /coded_by="XM_077645727.1:461..2833"
                     /db_xref="GeneID:144112910"
ORIGIN      
        1 mapakcveta vvathsiprp rdvpfkvcvp vrvtyyngpa kvflrynppg lpeypyekfc
       61 vgdgrrhlhe vfvgmlclvc qdgrmeeglr aqirsvhrns nglqalaril yvdtgyedfv
      121 eldclypida rataeprgtv ecclrrlrpt lrstrldwnm lpdgceyeai fhsvsdtgvy
      181 evdlfvvyst trmelvrpnv genlvyndya qfpsylydip amdgnpaqat srlaspscee
      241 gstktpetsp stlsdradtp segsascyel aaaeeptgys weikvtyist pdhwygqlts
      301 rskdllevcs iissscsesa cperevkkgs ylicrdfpha mgarvrveev ldgdrcfifl
      361 idygnrkvvs ssclfrldrr lrsinplalr fhltgiapws ewteaavtsf ealtqgdtkl
      421 tvevletqsc gdefddkvhl vklfsethgn vaecmirdgy armpierkvv pgqgtnfgdm
      481 qfdpmqddrn dvlnsygvnt ddpgvatskf tvkdghrick ffsahgtckn gqycvyshva
      541 eeansallhv sepvlslssi mqpppvlesw vfgqvsacrs psffflvfpy gqspieqliv
      601 edmasrskls leglmedlqr ecnlrkfseh rlftkaegel vaarsnrdar wyrgqvvsvg
      661 dsdvlqvffv dfgfyewvpe eevkaldvrf tylplqahpa civgdgfscp gdktgwdaem
      721 revflncvtc kdllveivri sdgllhvrly flqddklfnv tdcmtackrr stkeecrmhs
      781 iftktrrvps
//
LOCUS       XP_077501854             736 aa            linear   INV 25-NOV-2025
DEFINITION  tudor domain-containing protein 1-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501854
VERSION     XP_077501854.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645728.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..736
                     /product="tudor domain-containing protein 1-like isoform
                     X2"
                     /calculated_mol_wt=82544
     Region          <74..145
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          <344..377
                     /region_name="Tudor_dTUD-like"
                     /note="Tudor domain found in Drosophila melanogaster
                     maternal protein Tudor (dTUD) and similar proteins;
                     cd20379"
                     /db_xref="CDD:410450"
     Region          514..539
                     /region_name="zf-CCCH"
                     /note="Zinc finger C-x8-C-x5-C-x3-H type (and similar);
                     pfam00642"
                     /db_xref="CDD:459885"
     Region          575..704
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     CDS             1..736
                     /gene="LOC144112910"
                     /coded_by="XM_077645728.1:465..2675"
                     /db_xref="GeneID:144112910"
ORIGIN      
        1 mapakcveta vvathsiprp rdvpfkvcvp vrvtyyngpa kvflrynppg lpeypyekfc
       61 vgdgrrhlhe vfvgmlclvc qdgrmeeglr aqirsvhrns nglqalaril yvdtgyedfv
      121 eldclypida rataeprgtv ecclrrlrpt lrstrldwnm lpdgceyeai fhsvsdtgvy
      181 evdlfvvyst trmelvrpnv genlvyndya qfpsylydip amdgnpaqat srlaspscee
      241 gstktpetsp stlsdradtp segsascyel aaaeeptgys weikvtyist pdhwygqlts
      301 rskdllevcs iissscsesa cperevkkgs ylicrdfpha mgarvrveev ldgdrcfifl
      361 idygnrkvvs ssclfrldrr lrsinplalr fhltgiapws ewteaavtsf ealtqgdtkl
      421 tvevletqsc gdefddkvhl vklfsethgn vaecmirdgy armpierkvv pgqgtnfgdm
      481 qfdpmqddrn dvlnsygvnt ddpgvatskf tvkdghrick ffsahgtckn gqycvyshva
      541 eeansallhv sepvlslssi mqpppvlesw vfgqvsacrs psffflvfpy gqspieqliv
      601 edmasrskls leglmedlqr ecnlrkfseh rlftkaegel vaarsnrdar wyrgqvvsvg
      661 dsdvlqvffv dfgfyewvpe eevkaldvrf tylplqahpa civgdgfscp gdktgwdaem
      721 reecyrsdga dwfasr
//
LOCUS       XP_077501855             650 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112910 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501855
VERSION     XP_077501855.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645729.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..650
                     /product="uncharacterized protein LOC144112910 isoform X3"
                     /calculated_mol_wt=72637
     Region          <74..145
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          <344..377
                     /region_name="Tudor_dTUD-like"
                     /note="Tudor domain found in Drosophila melanogaster
                     maternal protein Tudor (dTUD) and similar proteins;
                     cd20379"
                     /db_xref="CDD:410450"
     Region          514..539
                     /region_name="zf-CCCH"
                     /note="Zinc finger C-x8-C-x5-C-x3-H type (and similar);
                     pfam00642"
                     /db_xref="CDD:459885"
     CDS             1..650
                     /gene="LOC144112910"
                     /coded_by="XM_077645729.1:473..2425"
                     /db_xref="GeneID:144112910"
ORIGIN      
        1 mapakcveta vvathsiprp rdvpfkvcvp vrvtyyngpa kvflrynppg lpeypyekfc
       61 vgdgrrhlhe vfvgmlclvc qdgrmeeglr aqirsvhrns nglqalaril yvdtgyedfv
      121 eldclypida rataeprgtv ecclrrlrpt lrstrldwnm lpdgceyeai fhsvsdtgvy
      181 evdlfvvyst trmelvrpnv genlvyndya qfpsylydip amdgnpaqat srlaspscee
      241 gstktpetsp stlsdradtp segsascyel aaaeeptgys weikvtyist pdhwygqlts
      301 rskdllevcs iissscsesa cperevkkgs ylicrdfpha mgarvrveev ldgdrcfifl
      361 idygnrkvvs ssclfrldrr lrsinplalr fhltgiapws ewteaavtsf ealtqgdtkl
      421 tvevletqsc gdefddkvhl vklfsethgn vaecmirdgy armpierkvv pgqgtnfgdm
      481 qfdpmqddrn dvlnsygvnt ddpgvatskf tvkdghrick ffsahgtckn gqycvyshva
      541 eeansallhv sepvlslssi mqpppvlesw vfgqvsacrs psffflvfpy gqspieqliv
      601 edmasrskls leglmedlqr mlqirwsrlv cleikavtss lvftpdpakk
//
LOCUS       XP_077501856             650 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112910 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501856
VERSION     XP_077501856.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645730.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..650
                     /product="uncharacterized protein LOC144112910 isoform X3"
                     /calculated_mol_wt=72637
     Region          <74..145
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          <344..377
                     /region_name="Tudor_dTUD-like"
                     /note="Tudor domain found in Drosophila melanogaster
                     maternal protein Tudor (dTUD) and similar proteins;
                     cd20379"
                     /db_xref="CDD:410450"
     Region          514..539
                     /region_name="zf-CCCH"
                     /note="Zinc finger C-x8-C-x5-C-x3-H type (and similar);
                     pfam00642"
                     /db_xref="CDD:459885"
     CDS             1..650
                     /gene="LOC144112910"
                     /coded_by="XM_077645730.1:473..2425"
                     /db_xref="GeneID:144112910"
ORIGIN      
        1 mapakcveta vvathsiprp rdvpfkvcvp vrvtyyngpa kvflrynppg lpeypyekfc
       61 vgdgrrhlhe vfvgmlclvc qdgrmeeglr aqirsvhrns nglqalaril yvdtgyedfv
      121 eldclypida rataeprgtv ecclrrlrpt lrstrldwnm lpdgceyeai fhsvsdtgvy
      181 evdlfvvyst trmelvrpnv genlvyndya qfpsylydip amdgnpaqat srlaspscee
      241 gstktpetsp stlsdradtp segsascyel aaaeeptgys weikvtyist pdhwygqlts
      301 rskdllevcs iissscsesa cperevkkgs ylicrdfpha mgarvrveev ldgdrcfifl
      361 idygnrkvvs ssclfrldrr lrsinplalr fhltgiapws ewteaavtsf ealtqgdtkl
      421 tvevletqsc gdefddkvhl vklfsethgn vaecmirdgy armpierkvv pgqgtnfgdm
      481 qfdpmqddrn dvlnsygvnt ddpgvatskf tvkdghrick ffsahgtckn gqycvyshva
      541 eeansallhv sepvlslssi mqpppvlesw vfgqvsacrs psffflvfpy gqspieqliv
      601 edmasrskls leglmedlqr mlqirwsrlv cleikavtss lvftpdpakk
//
LOCUS       XP_077501857             643 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112910 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077501857
VERSION     XP_077501857.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645731.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..643
                     /product="uncharacterized protein LOC144112910 isoform X4"
                     /calculated_mol_wt=72018
     Region          <74..145
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          <344..377
                     /region_name="Tudor_dTUD-like"
                     /note="Tudor domain found in Drosophila melanogaster
                     maternal protein Tudor (dTUD) and similar proteins;
                     cd20379"
                     /db_xref="CDD:410450"
     Region          514..539
                     /region_name="zf-CCCH"
                     /note="Zinc finger C-x8-C-x5-C-x3-H type (and similar);
                     pfam00642"
                     /db_xref="CDD:459885"
     CDS             1..643
                     /gene="LOC144112910"
                     /coded_by="XM_077645731.1:988..2919"
                     /db_xref="GeneID:144112910"
ORIGIN      
        1 mapakcveta vvathsiprp rdvpfkvcvp vrvtyyngpa kvflrynppg lpeypyekfc
       61 vgdgrrhlhe vfvgmlclvc qdgrmeeglr aqirsvhrns nglqalaril yvdtgyedfv
      121 eldclypida rataeprgtv ecclrrlrpt lrstrldwnm lpdgceyeai fhsvsdtgvy
      181 evdlfvvyst trmelvrpnv genlvyndya qfpsylydip amdgnpaqat srlaspscee
      241 gstktpetsp stlsdradtp segsascyel aaaeeptgys weikvtyist pdhwygqlts
      301 rskdllevcs iissscsesa cperevkkgs ylicrdfpha mgarvrveev ldgdrcfifl
      361 idygnrkvvs ssclfrldrr lrsinplalr fhltgiapws ewteaavtsf ealtqgdtkl
      421 tvevletqsc gdefddkvhl vklfsethgn vaecmirdgy armpierkvv pgqgtnfgdm
      481 qfdpmqddrn dvlnsygvnt ddpgvatskf tvkdghrick ffsahgtckn gqycvyshva
      541 eeansallha hpacivgdgf scpgdktgwd aemrevflnc vtckdllvei vrisdgllhv
      601 rlyflqddkl fnvtdcmtac krrstkeecr mhsiftktrr vps
//
LOCUS       XP_077501858             589 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112910 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077501858
VERSION     XP_077501858.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645732.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..589
                     /product="uncharacterized protein LOC144112910 isoform X5"
                     /calculated_mol_wt=65686
     Region          <74..145
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          <344..377
                     /region_name="Tudor_dTUD-like"
                     /note="Tudor domain found in Drosophila melanogaster
                     maternal protein Tudor (dTUD) and similar proteins;
                     cd20379"
                     /db_xref="CDD:410450"
     Region          514..539
                     /region_name="zf-CCCH"
                     /note="Zinc finger C-x8-C-x5-C-x3-H type (and similar);
                     pfam00642"
                     /db_xref="CDD:459885"
     CDS             1..589
                     /gene="LOC144112910"
                     /coded_by="XM_077645732.1:988..2757"
                     /db_xref="GeneID:144112910"
ORIGIN      
        1 mapakcveta vvathsiprp rdvpfkvcvp vrvtyyngpa kvflrynppg lpeypyekfc
       61 vgdgrrhlhe vfvgmlclvc qdgrmeeglr aqirsvhrns nglqalaril yvdtgyedfv
      121 eldclypida rataeprgtv ecclrrlrpt lrstrldwnm lpdgceyeai fhsvsdtgvy
      181 evdlfvvyst trmelvrpnv genlvyndya qfpsylydip amdgnpaqat srlaspscee
      241 gstktpetsp stlsdradtp segsascyel aaaeeptgys weikvtyist pdhwygqlts
      301 rskdllevcs iissscsesa cperevkkgs ylicrdfpha mgarvrveev ldgdrcfifl
      361 idygnrkvvs ssclfrldrr lrsinplalr fhltgiapws ewteaavtsf ealtqgdtkl
      421 tvevletqsc gdefddkvhl vklfsethgn vaecmirdgy armpierkvv pgqgtnfgdm
      481 qfdpmqddrn dvlnsygvnt ddpgvatskf tvkdghrick ffsahgtckn gqycvyshva
      541 eeansallha hpacivgdgf scpgdktgwd aemreecyrs dgadwfasr
//
LOCUS       XP_077501859             790 aa            linear   INV 25-NOV-2025
DEFINITION  tudor domain-containing protein 1-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501859
VERSION     XP_077501859.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645733.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..790
                     /product="tudor domain-containing protein 1-like isoform
                     X1"
                     /calculated_mol_wt=88889
     Region          <74..145
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          <344..377
                     /region_name="Tudor_dTUD-like"
                     /note="Tudor domain found in Drosophila melanogaster
                     maternal protein Tudor (dTUD) and similar proteins;
                     cd20379"
                     /db_xref="CDD:410450"
     Region          514..539
                     /region_name="zf-CCCH"
                     /note="Zinc finger C-x8-C-x5-C-x3-H type (and similar);
                     pfam00642"
                     /db_xref="CDD:459885"
     Region          575..704
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     CDS             1..790
                     /gene="LOC144112912"
                     /coded_by="XM_077645733.1:416..2788"
                     /db_xref="GeneID:144112912"
ORIGIN      
        1 mapakcveta vvathsiprp rdvpfkvcvp vrvtyyngpa kvflrynppg lpeypyekfc
       61 vgdgrrhlhe vfvgmlclvc qdgrmeeglr aqirsvhrns nglqalaril yvdtgyedfv
      121 eldclypida rataeprgtv ecclrrlrpt lrstrldwnm lpdgceyeai fhsvsdtgvy
      181 evdlfvvyst trmelvrpnv genlvyndya qfpsylydip amdgnpaqat srlaspscee
      241 gstktpetsp stlsdradtp segsascyel aaaeeptgys weikvtyist pdhwygqlts
      301 rskdllevcs iissscsesa cperevkkgs ylicrdfpha mgarvrveev ldgdrcfifl
      361 idygnrkvvs ssclfrldrr lrsinplalr fhltgiapws ewteaavtsf ealtqgdtkl
      421 tvevletqsc gdefddkvhl vklfsethgn vaecmirdgy armpierkvi pgqgtnfgdm
      481 qfdpmqddrn dvlnsygvnt ddpgvatskf tvkdghrick ffsahgtckn gqycvyshva
      541 eeansallhv sepvlslssi mqpppvlesw vfgqvsacrs psffflvfpy gqspieqliv
      601 edmasrskls leglmedlqr ecnlrkfseh rlftkaegel vaarsnrdar wyrgqvvsvg
      661 dsdvlqvffv dfgfyewvpe eevkaldvrf tylplqahpa civgdgfscp gdktgwdaem
      721 revflncvtc kdllveivri sdgllhvrly flqddklfnv tdcmtackrr stkeecrmhs
      781 iftktrrvps
//
LOCUS       XP_077501860             736 aa            linear   INV 25-NOV-2025
DEFINITION  tudor domain-containing protein 1-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501860
VERSION     XP_077501860.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645734.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..736
                     /product="tudor domain-containing protein 1-like isoform
                     X2"
                     /calculated_mol_wt=82558
     Region          <74..145
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          <344..377
                     /region_name="Tudor_dTUD-like"
                     /note="Tudor domain found in Drosophila melanogaster
                     maternal protein Tudor (dTUD) and similar proteins;
                     cd20379"
                     /db_xref="CDD:410450"
     Region          514..539
                     /region_name="zf-CCCH"
                     /note="Zinc finger C-x8-C-x5-C-x3-H type (and similar);
                     pfam00642"
                     /db_xref="CDD:459885"
     Region          575..704
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     CDS             1..736
                     /gene="LOC144112912"
                     /coded_by="XM_077645734.1:418..2628"
                     /db_xref="GeneID:144112912"
ORIGIN      
        1 mapakcveta vvathsiprp rdvpfkvcvp vrvtyyngpa kvflrynppg lpeypyekfc
       61 vgdgrrhlhe vfvgmlclvc qdgrmeeglr aqirsvhrns nglqalaril yvdtgyedfv
      121 eldclypida rataeprgtv ecclrrlrpt lrstrldwnm lpdgceyeai fhsvsdtgvy
      181 evdlfvvyst trmelvrpnv genlvyndya qfpsylydip amdgnpaqat srlaspscee
      241 gstktpetsp stlsdradtp segsascyel aaaeeptgys weikvtyist pdhwygqlts
      301 rskdllevcs iissscsesa cperevkkgs ylicrdfpha mgarvrveev ldgdrcfifl
      361 idygnrkvvs ssclfrldrr lrsinplalr fhltgiapws ewteaavtsf ealtqgdtkl
      421 tvevletqsc gdefddkvhl vklfsethgn vaecmirdgy armpierkvi pgqgtnfgdm
      481 qfdpmqddrn dvlnsygvnt ddpgvatskf tvkdghrick ffsahgtckn gqycvyshva
      541 eeansallhv sepvlslssi mqpppvlesw vfgqvsacrs psffflvfpy gqspieqliv
      601 edmasrskls leglmedlqr ecnlrkfseh rlftkaegel vaarsnrdar wyrgqvvsvg
      661 dsdvlqvffv dfgfyewvpe eevkaldvrf tylplqahpa civgdgfscp gdktgwdaem
      721 reecyrsdga dwfasr
//
LOCUS       XP_077501861             650 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112912 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501861
VERSION     XP_077501861.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645735.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..650
                     /product="uncharacterized protein LOC144112912 isoform X3"
                     /calculated_mol_wt=72651
     Region          <74..145
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          <344..377
                     /region_name="Tudor_dTUD-like"
                     /note="Tudor domain found in Drosophila melanogaster
                     maternal protein Tudor (dTUD) and similar proteins;
                     cd20379"
                     /db_xref="CDD:410450"
     Region          514..539
                     /region_name="zf-CCCH"
                     /note="Zinc finger C-x8-C-x5-C-x3-H type (and similar);
                     pfam00642"
                     /db_xref="CDD:459885"
     CDS             1..650
                     /gene="LOC144112912"
                     /coded_by="XM_077645735.1:422..2374"
                     /db_xref="GeneID:144112912"
ORIGIN      
        1 mapakcveta vvathsiprp rdvpfkvcvp vrvtyyngpa kvflrynppg lpeypyekfc
       61 vgdgrrhlhe vfvgmlclvc qdgrmeeglr aqirsvhrns nglqalaril yvdtgyedfv
      121 eldclypida rataeprgtv ecclrrlrpt lrstrldwnm lpdgceyeai fhsvsdtgvy
      181 evdlfvvyst trmelvrpnv genlvyndya qfpsylydip amdgnpaqat srlaspscee
      241 gstktpetsp stlsdradtp segsascyel aaaeeptgys weikvtyist pdhwygqlts
      301 rskdllevcs iissscsesa cperevkkgs ylicrdfpha mgarvrveev ldgdrcfifl
      361 idygnrkvvs ssclfrldrr lrsinplalr fhltgiapws ewteaavtsf ealtqgdtkl
      421 tvevletqsc gdefddkvhl vklfsethgn vaecmirdgy armpierkvi pgqgtnfgdm
      481 qfdpmqddrn dvlnsygvnt ddpgvatskf tvkdghrick ffsahgtckn gqycvyshva
      541 eeansallhv sepvlslssi mqpppvlesw vfgqvsacrs psffflvfpy gqspieqliv
      601 edmasrskls leglmedlqr mlqirwsrlv cleikavtss lvftpdpakk
//
LOCUS       XP_077501862             650 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112912 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501862
VERSION     XP_077501862.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645736.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..650
                     /product="uncharacterized protein LOC144112912 isoform X3"
                     /calculated_mol_wt=72651
     Region          <74..145
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          <344..377
                     /region_name="Tudor_dTUD-like"
                     /note="Tudor domain found in Drosophila melanogaster
                     maternal protein Tudor (dTUD) and similar proteins;
                     cd20379"
                     /db_xref="CDD:410450"
     Region          514..539
                     /region_name="zf-CCCH"
                     /note="Zinc finger C-x8-C-x5-C-x3-H type (and similar);
                     pfam00642"
                     /db_xref="CDD:459885"
     CDS             1..650
                     /gene="LOC144112912"
                     /coded_by="XM_077645736.1:422..2374"
                     /db_xref="GeneID:144112912"
ORIGIN      
        1 mapakcveta vvathsiprp rdvpfkvcvp vrvtyyngpa kvflrynppg lpeypyekfc
       61 vgdgrrhlhe vfvgmlclvc qdgrmeeglr aqirsvhrns nglqalaril yvdtgyedfv
      121 eldclypida rataeprgtv ecclrrlrpt lrstrldwnm lpdgceyeai fhsvsdtgvy
      181 evdlfvvyst trmelvrpnv genlvyndya qfpsylydip amdgnpaqat srlaspscee
      241 gstktpetsp stlsdradtp segsascyel aaaeeptgys weikvtyist pdhwygqlts
      301 rskdllevcs iissscsesa cperevkkgs ylicrdfpha mgarvrveev ldgdrcfifl
      361 idygnrkvvs ssclfrldrr lrsinplalr fhltgiapws ewteaavtsf ealtqgdtkl
      421 tvevletqsc gdefddkvhl vklfsethgn vaecmirdgy armpierkvi pgqgtnfgdm
      481 qfdpmqddrn dvlnsygvnt ddpgvatskf tvkdghrick ffsahgtckn gqycvyshva
      541 eeansallhv sepvlslssi mqpppvlesw vfgqvsacrs psffflvfpy gqspieqliv
      601 edmasrskls leglmedlqr mlqirwsrlv cleikavtss lvftpdpakk
//
LOCUS       XP_077501863             643 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112912 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077501863
VERSION     XP_077501863.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645737.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..643
                     /product="uncharacterized protein LOC144112912 isoform X4"
                     /calculated_mol_wt=72032
     Region          <74..145
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          <344..377
                     /region_name="Tudor_dTUD-like"
                     /note="Tudor domain found in Drosophila melanogaster
                     maternal protein Tudor (dTUD) and similar proteins;
                     cd20379"
                     /db_xref="CDD:410450"
     Region          514..539
                     /region_name="zf-CCCH"
                     /note="Zinc finger C-x8-C-x5-C-x3-H type (and similar);
                     pfam00642"
                     /db_xref="CDD:459885"
     CDS             1..643
                     /gene="LOC144112912"
                     /coded_by="XM_077645737.1:424..2355"
                     /db_xref="GeneID:144112912"
ORIGIN      
        1 mapakcveta vvathsiprp rdvpfkvcvp vrvtyyngpa kvflrynppg lpeypyekfc
       61 vgdgrrhlhe vfvgmlclvc qdgrmeeglr aqirsvhrns nglqalaril yvdtgyedfv
      121 eldclypida rataeprgtv ecclrrlrpt lrstrldwnm lpdgceyeai fhsvsdtgvy
      181 evdlfvvyst trmelvrpnv genlvyndya qfpsylydip amdgnpaqat srlaspscee
      241 gstktpetsp stlsdradtp segsascyel aaaeeptgys weikvtyist pdhwygqlts
      301 rskdllevcs iissscsesa cperevkkgs ylicrdfpha mgarvrveev ldgdrcfifl
      361 idygnrkvvs ssclfrldrr lrsinplalr fhltgiapws ewteaavtsf ealtqgdtkl
      421 tvevletqsc gdefddkvhl vklfsethgn vaecmirdgy armpierkvi pgqgtnfgdm
      481 qfdpmqddrn dvlnsygvnt ddpgvatskf tvkdghrick ffsahgtckn gqycvyshva
      541 eeansallha hpacivgdgf scpgdktgwd aemrevflnc vtckdllvei vrisdgllhv
      601 rlyflqddkl fnvtdcmtac krrstkeecr mhsiftktrr vps
//
LOCUS       XP_077501864             589 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112912 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077501864
VERSION     XP_077501864.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645738.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..589
                     /product="uncharacterized protein LOC144112912 isoform X5"
                     /calculated_mol_wt=65700
     Region          <74..145
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          <344..377
                     /region_name="Tudor_dTUD-like"
                     /note="Tudor domain found in Drosophila melanogaster
                     maternal protein Tudor (dTUD) and similar proteins;
                     cd20379"
                     /db_xref="CDD:410450"
     Region          514..539
                     /region_name="zf-CCCH"
                     /note="Zinc finger C-x8-C-x5-C-x3-H type (and similar);
                     pfam00642"
                     /db_xref="CDD:459885"
     CDS             1..589
                     /gene="LOC144112912"
                     /coded_by="XM_077645738.1:428..2197"
                     /db_xref="GeneID:144112912"
ORIGIN      
        1 mapakcveta vvathsiprp rdvpfkvcvp vrvtyyngpa kvflrynppg lpeypyekfc
       61 vgdgrrhlhe vfvgmlclvc qdgrmeeglr aqirsvhrns nglqalaril yvdtgyedfv
      121 eldclypida rataeprgtv ecclrrlrpt lrstrldwnm lpdgceyeai fhsvsdtgvy
      181 evdlfvvyst trmelvrpnv genlvyndya qfpsylydip amdgnpaqat srlaspscee
      241 gstktpetsp stlsdradtp segsascyel aaaeeptgys weikvtyist pdhwygqlts
      301 rskdllevcs iissscsesa cperevkkgs ylicrdfpha mgarvrveev ldgdrcfifl
      361 idygnrkvvs ssclfrldrr lrsinplalr fhltgiapws ewteaavtsf ealtqgdtkl
      421 tvevletqsc gdefddkvhl vklfsethgn vaecmirdgy armpierkvi pgqgtnfgdm
      481 qfdpmqddrn dvlnsygvnt ddpgvatskf tvkdghrick ffsahgtckn gqycvyshva
      541 eeansallha hpacivgdgf scpgdktgwd aemreecyrs dgadwfasr
//
LOCUS       XP_077501865             290 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112913 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501865
VERSION     XP_077501865.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645739.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..290
                     /product="uncharacterized protein LOC144112913 isoform X1"
                     /calculated_mol_wt=32720
     Region          237..>290
                     /region_name="GIY-YIG_SF"
                     /note="GIY-YIG nuclease domain superfamily; cl15257"
                     /db_xref="CDD:472790"
     Site            order(237..239,250..252)
                     /site_type="other"
                     /note="GIY-YIG motif/motif A"
                     /db_xref="CDD:198380"
     Site            order(239,250,252,260,264)
                     /site_type="active"
                     /db_xref="CDD:198380"
     CDS             1..290
                     /gene="LOC144112913"
                     /coded_by="XM_077645739.1:2198..3070"
                     /db_xref="GeneID:144112913"
ORIGIN      
        1 mqccqnrrys qvqcrprpfp tnamlcsccl lletcaedap hlagpscivv tltwpafvri
       61 anlnsiervv qftvekeedg qlpfldvlvk rsqsmlsfkv yresthtgcy lhfhslhpas
      121 hkrsvvaslf hrayrictep edvkadhrsv rkdfsecgyp lpfvnaverk llqpprsdss
      181 paakkcapvp ympgineala rilrsydsev shvpakklrq alvnvkyclp kekfpgivyt
      241 ipcrdcnhvy mgesgnfvkr lkqhqhdvan kkassnalae hsesqqhdvd
//
LOCUS       XP_077501866             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112913 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501866
VERSION     XP_077501866.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645740.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144112913 isoform X2"
                     /calculated_mol_wt=14009
     CDS             1..127
                     /gene="LOC144112913"
                     /coded_by="XM_077645740.1:4371..4754"
                     /db_xref="GeneID:144112913"
ORIGIN      
        1 miasmstspq rphylrtapt pnalnglgst atmstyahlr rlfvmqslts gvlpqdwkkg
       61 asiisaahhl ssrihhsktv ypppartash lrsffvkssr dwndlppeai hltnltkfkk
      121 kieaiil
//
LOCUS       XP_077501867             290 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112914 [Amblyomma americanum].
ACCESSION   XP_077501867
VERSION     XP_077501867.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645741.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..290
                     /product="uncharacterized protein LOC144112914"
                     /calculated_mol_wt=32678
     Region          237..>290
                     /region_name="GIY-YIG_SF"
                     /note="GIY-YIG nuclease domain superfamily; cl15257"
                     /db_xref="CDD:472790"
     Site            order(237..239,250..252)
                     /site_type="other"
                     /note="GIY-YIG motif/motif A"
                     /db_xref="CDD:198380"
     Site            order(239,250,252,260,264)
                     /site_type="active"
                     /db_xref="CDD:198380"
     CDS             1..290
                     /gene="LOC144112914"
                     /coded_by="XM_077645741.1:1..873"
                     /db_xref="GeneID:144112914"
ORIGIN      
        1 mqccqnrrys qvqcrprpfp tnamlcsccl lletcaedap hlagpscivv tltwpafvri
       61 anlnsiervv qftvekeedg qlpfldvlvk rsqsmlsfkv yresthtgcy lhfhslhpas
      121 hkrsvvaslf hrayrictep edvkadhrsv rkdfsecgyp lpfvnaverk llqpprsdss
      181 paakkcapvp ympgineala rilrsydsev shvpakklrq algnvkyclp kekfpgivyt
      241 ipcrdcnhvy mgesgnfvkr lkqhqhdvan kkassnalae hsesqqhdvd
//
LOCUS       XP_077501868             267 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112916 [Amblyomma americanum].
ACCESSION   XP_077501868
VERSION     XP_077501868.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645742.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..267
                     /product="uncharacterized protein LOC144112916"
                     /calculated_mol_wt=29954
     Region          214..>267
                     /region_name="GIY-YIG_SF"
                     /note="GIY-YIG nuclease domain superfamily; cl15257"
                     /db_xref="CDD:472790"
     Site            order(214..216,227..229)
                     /site_type="other"
                     /note="GIY-YIG motif/motif A"
                     /db_xref="CDD:198380"
     Site            order(216,227,229,237,241)
                     /site_type="active"
                     /db_xref="CDD:198380"
     CDS             1..267
                     /gene="LOC144112916"
                     /coded_by="XM_077645742.1:371..1174"
                     /db_xref="GeneID:144112916"
ORIGIN      
        1 mlcsccllle tcaedaphla gpscivvtlt wpafvrianl nsiervvqft vekeedgqlp
       61 fldvlvkrsq smlsfkvyre sthtgcylhf hslhpashkr svvaslfhra yrictepedv
      121 kadhrsvrkd fsecgyplpf vnaverkllq pprsdsspaa kkcapvpymp ginealaril
      181 rsydsevshv pakklrqalv nvkyclpkek fpgivytipc rdcnhvymge sgnfvkrlkq
      241 hqhdvankka ssnalaehse sqqhdvd
//
LOCUS       XP_077501869             267 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112917 [Amblyomma americanum].
ACCESSION   XP_077501869
VERSION     XP_077501869.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645743.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..267
                     /product="uncharacterized protein LOC144112917"
                     /calculated_mol_wt=29799
     Region          214..>267
                     /region_name="GIY-YIG_SF"
                     /note="GIY-YIG nuclease domain superfamily; cl15257"
                     /db_xref="CDD:472790"
     Site            order(214..216,227..229)
                     /site_type="other"
                     /note="GIY-YIG motif/motif A"
                     /db_xref="CDD:198380"
     Site            order(216,227,229,237,241)
                     /site_type="active"
                     /db_xref="CDD:198380"
     CDS             1..267
                     /gene="LOC144112917"
                     /coded_by="XM_077645743.1:3..806"
                     /db_xref="GeneID:144112917"
ORIGIN      
        1 mlcsccllle tcaedaphla gpscivvtlt wpafvriahl nsiervvqlt vekeedgqlp
       61 fldvlvkrsq smlsfkvyre sthtgcylhf hslppashkr svvaslfhra yrictepedv
      121 kadhrsvrkd lsecgyplpf vnaverkllq pprsdsspaa kkcapvpymp ginealaril
      181 rsydsevshv pakklrqalv nvkyclpken fpgivytipc rdcnhvyvge sgnfvkrlkq
      241 nqhdvankka ssnalaehse sqqhdvd
//
LOCUS       XP_077501870             267 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112918 [Amblyomma americanum].
ACCESSION   XP_077501870
VERSION     XP_077501870.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645744.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..267
                     /product="uncharacterized protein LOC144112918"
                     /calculated_mol_wt=29739
     Region          214..>267
                     /region_name="GIY-YIG_SF"
                     /note="GIY-YIG nuclease domain superfamily; cl15257"
                     /db_xref="CDD:472790"
     Site            order(214..216,227..229)
                     /site_type="other"
                     /note="GIY-YIG motif/motif A"
                     /db_xref="CDD:198380"
     Site            order(216,227,229,237,241)
                     /site_type="active"
                     /db_xref="CDD:198380"
     CDS             1..267
                     /gene="LOC144112918"
                     /coded_by="XM_077645744.1:559..1362"
                     /db_xref="GeneID:144112918"
ORIGIN      
        1 mlcsccllle tcaedaphla gpscivvtlt wpafvriahl nsiervvqlt vekeedgqlp
       61 fldvlvkrsq smlsfkvyre sthtgcylhf hslppashkr svvaslfhra yrictepedv
      121 kadhrsvrkd lsecgcplpf vnaverkllq pprsdsspaa kkcapvpymp ginealaril
      181 rsydsevshv pakklrqalv nvkyclpken fpgivytipc rdcnhvyvge sgnfvkrlkq
      241 nqhdvankka ssnalaehse sqqhdvd
//
LOCUS       XP_077501871             102 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112919 [Amblyomma americanum].
ACCESSION   XP_077501871
VERSION     XP_077501871.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645745.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..102
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..102
                     /product="uncharacterized protein LOC144112919"
                     /calculated_mol_wt=10570
     CDS             1..102
                     /gene="LOC144112919"
                     /coded_by="XM_077645745.1:88..396"
                     /db_xref="GeneID:144112919"
ORIGIN      
        1 mlpacgecks prsdtssdwl twrlatvpef pgsnptaaaa lskeakrkga rvlcdvlalv
       61 pfsppagaca daswaigntl lpvpspyhpw snnawlcipa gp
//
LOCUS       XP_077501872             884 aa            linear   INV 25-NOV-2025
DEFINITION  glycerol-3-phosphate acyltransferase mino [Amblyomma americanum].
ACCESSION   XP_077501872
VERSION     XP_077501872.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645746.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (GKSP01013410.1) annotated
            using gene prediction method: Gnomon, supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 173 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..884
                     /product="glycerol-3-phosphate acyltransferase mino"
                     /calculated_mol_wt=99493
     Region          190..850
                     /region_name="PlsB"
                     /note="Glycerol-3-phosphate O-acyltransferase [Lipid
                     transport and metabolism]; COG2937"
                     /db_xref="CDD:442180"
     Region          254..461
                     /region_name="LPLAT_DHAPAT-like"
                     /note="Lysophospholipid Acyltransferases (LPLATs) of
                     Glycerophospholipid Biosynthesis: GPAT-like; cd07993"
                     /db_xref="CDD:153255"
     Site            order(281,284,286,307..310,366..368)
                     /site_type="active"
                     /note="putative acyl-acceptor binding pocket [active]"
                     /db_xref="CDD:153255"
     CDS             1..884
                     /gene="mino"
                     /coded_by="XM_077645746.1:195..2849"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 173 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144112921"
ORIGIN      
        1 mltalvlfvs vylavralmv kllqnlhday smwdfksgpr vtdsfssscg srgrehqqrr
       61 srrdrpssqv aafnpnkygv dsirvpmaaa rrakgclvde vlpyfkkwap mehepqseal
      121 rfvslescek cqpvtksqfk idstlalqsl dnalivpssc rswltpiefi knsiyltckn
      181 ipheytpipk eiltsesied aiskaveddp esdrpehrkr alsifgrmra sistvllrva
      241 vwfmhrimsr mltgvyvpkg qiemlkrave qnvpmvylpl hrshldyilv tyllylngmr
      301 vplvaagdnl liplfgtllr glggffikrr ldfkdgrkdh lyravlkeym keilkgnhsl
      361 effleggrsr tgktrlpkag llsvivncvn eglvkdvyiv pisisyekll dgnfvseqlg
      421 epkvmetfta altsiwrilh snygsvrvdf cqpislnefl esskdvstep lcfdcplall
      481 dvtrsstspt eykvcastss lasadslped crlaikslan hvtcqssasl almstnllaf
      541 llltkhrkgg tlqqlaysls wlrmavsdrq hqvmcpadsa eavrhacall gkelvltetv
      601 qmewssgdte nnvkivfyrp alhlpsvlel qyyanavlsv fvheaiaata lftltgdyia
      661 lmrgrdnlvv vsrsdllnkc qdlinilqle fvlvlpcsna eevvnkaiys lieknilsvc
      721 qcpgplvltg astrstyrvp ycykfddese edkdlsfqdt qyrvvlnetn igmleflhsv
      781 lasyiesywv vacslsklvg tqieeriffr hiqkvaqdkl qqgtlyyees faadtlrnav
      841 qlyiqwgive qhsqdgmrvl ylgepwnvde aidsviafvg afrr
//
LOCUS       XP_077501873             419 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112922 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501873
VERSION     XP_077501873.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645747.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..419
                     /product="uncharacterized protein LOC144112922 isoform X1"
                     /calculated_mol_wt=46811
     Region          235..401
                     /region_name="PRX_Typ2cys"
                     /note="Peroxiredoxin (PRX) family, Typical 2-Cys PRX
                     subfamily; PRXs are thiol-specific antioxidant (TSA)
                     proteins, which confer a protective role in cells through
                     its peroxidase activity by reducing hydrogen peroxide,
                     peroxynitrite, and organic hydroperoxides; cd03015"
                     /db_xref="CDD:239313"
     Site            order(270,304..305,330,332,344)
                     /site_type="other"
                     /note="decamer (pentamer of dimers) interface [polypeptide
                     binding]"
                     /db_xref="CDD:239313"
     Site            order(271,274,350)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:239313"
     Site            order(272..273,276,339,349,362..364,366..368,372..373,380,
                     395..398)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239313"
     Site            order(274,395)
                     /site_type="active"
                     /note="peroxidatic and resolving cysteines [active]"
                     /db_xref="CDD:239313"
     CDS             1..419
                     /gene="LOC144112922"
                     /coded_by="XM_077645747.1:126..1385"
                     /db_xref="GeneID:144112922"
ORIGIN      
        1 matverwdvp sgvpgvlygh eafeqcrssk nyaiqqplri yeqmeeqvta lvplhsnqva
       61 evtaetrssk rkagessnts ggikwykqkf rdswlqderf knwlvsvpdd pyrakcrlcn
      121 adlragksel ekhartkhhr dtvkavedvk allgfanqag aqsphdvvtd mqsndqsnns
      181 fqmqddelsr pvpvasvvtq rrraspvpvp tvvsapavrc qvppmgflpr vqchapdfkg
      241 iavvdsqire iqlsdyegkf llllfypqdf slacpselve yseraaefrn lnteilaist
      301 dsycthlawt ntprklgglg kvnvtllsdf tkkiskdydv lledigialr asfiidpkgm
      361 vrqvtvndvn lyrsvdetlr llkalqhvek hgaaicaswe pepaggrgce rsarsrqdk
//
LOCUS       XP_077501874             418 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112922 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501874
VERSION     XP_077501874.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645748.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..418
                     /product="uncharacterized protein LOC144112922 isoform X2"
                     /calculated_mol_wt=46742
     Region          234..400
                     /region_name="PRX_Typ2cys"
                     /note="Peroxiredoxin (PRX) family, Typical 2-Cys PRX
                     subfamily; PRXs are thiol-specific antioxidant (TSA)
                     proteins, which confer a protective role in cells through
                     its peroxidase activity by reducing hydrogen peroxide,
                     peroxynitrite, and organic hydroperoxides; cd03015"
                     /db_xref="CDD:239313"
     Site            order(269,303..304,329,331,343)
                     /site_type="other"
                     /note="decamer (pentamer of dimers) interface [polypeptide
                     binding]"
                     /db_xref="CDD:239313"
     Site            order(270,273,349)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:239313"
     Site            order(271..272,275,338,348,361..363,365..367,371..372,379,
                     394..397)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239313"
     Site            order(273,394)
                     /site_type="active"
                     /note="peroxidatic and resolving cysteines [active]"
                     /db_xref="CDD:239313"
     CDS             1..418
                     /gene="LOC144112922"
                     /coded_by="XM_077645748.1:127..1383"
                     /db_xref="GeneID:144112922"
ORIGIN      
        1 matverwdvp sgvpgvlygh eafeqcrssk nyaiqqplri yeqmeeqvta lvplhsnqva
       61 evtaetrssk rkagessnts ggikwykqkf rdswlqderf knwlvsvpdd pyrakcrlcn
      121 adlragksel ekhartkhhr dtvkavedvk allgfanqag aqsphdvvtd mqsndqsnns
      181 fqmqddelsr pvpvasvvtq rrraspvpvp mvsapavrcq vppmgflprv qchapdfkgi
      241 avvdsqirei qlsdyegkfl lllfypqdfs lacpselvey seraaefrnl nteilaistd
      301 sycthlawtn tprklgglgk vnvtllsdft kkiskdydvl ledigialra sfiidpkgmv
      361 rqvtvndvnl yrsvdetlrl lkalqhvekh gaaicaswep epaggrgcer sarsrqdk
//
LOCUS       XP_077501875             413 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112922 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501875
VERSION     XP_077501875.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645749.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..413
                     /product="uncharacterized protein LOC144112922 isoform X3"
                     /calculated_mol_wt=46134
     Region          229..395
                     /region_name="PRX_Typ2cys"
                     /note="Peroxiredoxin (PRX) family, Typical 2-Cys PRX
                     subfamily; PRXs are thiol-specific antioxidant (TSA)
                     proteins, which confer a protective role in cells through
                     its peroxidase activity by reducing hydrogen peroxide,
                     peroxynitrite, and organic hydroperoxides; cd03015"
                     /db_xref="CDD:239313"
     Site            order(264,298..299,324,326,338)
                     /site_type="other"
                     /note="decamer (pentamer of dimers) interface [polypeptide
                     binding]"
                     /db_xref="CDD:239313"
     Site            order(265,268,344)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:239313"
     Site            order(266..267,270,333,343,356..358,360..362,366..367,374,
                     389..392)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239313"
     Site            order(268,389)
                     /site_type="active"
                     /note="peroxidatic and resolving cysteines [active]"
                     /db_xref="CDD:239313"
     CDS             1..413
                     /gene="LOC144112922"
                     /coded_by="XM_077645749.1:126..1367"
                     /db_xref="GeneID:144112922"
ORIGIN      
        1 matverwdvp sgvpgvlygh eafeqcrssk nyaiqqplri yeqmeeqvta lvplhsnqva
       61 evtaetrssk rkagessnts ggikwykqkf rdswlqderf knwlvsvpdd pyrakcrlcn
      121 adlragksel ekhartkhhr dtvkavedvk allgfanqag aqsphdvvtd mqsndqmqdd
      181 elsrpvpvas vvtqrrrasp vpvptvvsap avrcqvppmg flprvqchap dfkgiavvds
      241 qireiqlsdy egkfllllfy pqdfslacps elveyseraa efrnlnteil aistdsycth
      301 lawtntprkl gglgkvnvtl lsdftkkisk dydvlledig ialrasfiid pkgmvrqvtv
      361 ndvnlyrsvd etlrllkalq hvekhgaaic aswepepagg rgcersarsr qdk
//
LOCUS       XP_077501876             412 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112922 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077501876
VERSION     XP_077501876.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645750.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..412
                     /product="uncharacterized protein LOC144112922 isoform X4"
                     /calculated_mol_wt=46064
     Region          228..394
                     /region_name="PRX_Typ2cys"
                     /note="Peroxiredoxin (PRX) family, Typical 2-Cys PRX
                     subfamily; PRXs are thiol-specific antioxidant (TSA)
                     proteins, which confer a protective role in cells through
                     its peroxidase activity by reducing hydrogen peroxide,
                     peroxynitrite, and organic hydroperoxides; cd03015"
                     /db_xref="CDD:239313"
     Site            order(263,297..298,323,325,337)
                     /site_type="other"
                     /note="decamer (pentamer of dimers) interface [polypeptide
                     binding]"
                     /db_xref="CDD:239313"
     Site            order(264,267,343)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:239313"
     Site            order(265..266,269,332,342,355..357,359..361,365..366,373,
                     388..391)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239313"
     Site            order(267,388)
                     /site_type="active"
                     /note="peroxidatic and resolving cysteines [active]"
                     /db_xref="CDD:239313"
     CDS             1..412
                     /gene="LOC144112922"
                     /coded_by="XM_077645750.1:129..1367"
                     /db_xref="GeneID:144112922"
ORIGIN      
        1 matverwdvp sgvpgvlygh eafeqcrssk nyaiqqplri yeqmeeqvta lvplhsnqva
       61 evtaetrssk rkagessnts ggikwykqkf rdswlqderf knwlvsvpdd pyrakcrlcn
      121 adlragksel ekhartkhhr dtvkavedvk allgfanqag aqsphdvvtd mqsndqmqdd
      181 elsrpvpvas vvtqrrrasp vpvpmvsapa vrcqvppmgf lprvqchapd fkgiavvdsq
      241 ireiqlsdye gkfllllfyp qdfslacpse lveyseraae frnlnteila istdsycthl
      301 awtntprklg glgkvnvtll sdftkkiskd ydvlledigi alrasfiidp kgmvrqvtvn
      361 dvnlyrsvde tlrllkalqh vekhgaaica swepepaggr gcersarsrq dk
//
LOCUS       XP_077501877             407 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112922 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077501877
VERSION     XP_077501877.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645751.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..407
                     /product="uncharacterized protein LOC144112922 isoform X5"
                     /calculated_mol_wt=45156
     Region          223..389
                     /region_name="PRX_Typ2cys"
                     /note="Peroxiredoxin (PRX) family, Typical 2-Cys PRX
                     subfamily; PRXs are thiol-specific antioxidant (TSA)
                     proteins, which confer a protective role in cells through
                     its peroxidase activity by reducing hydrogen peroxide,
                     peroxynitrite, and organic hydroperoxides; cd03015"
                     /db_xref="CDD:239313"
     Site            order(258,292..293,318,320,332)
                     /site_type="other"
                     /note="decamer (pentamer of dimers) interface [polypeptide
                     binding]"
                     /db_xref="CDD:239313"
     Site            order(259,262,338)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:239313"
     Site            order(260..261,264,327,337,350..352,354..356,360..361,368,
                     383..386)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239313"
     Site            order(262,383)
                     /site_type="active"
                     /note="peroxidatic and resolving cysteines [active]"
                     /db_xref="CDD:239313"
     CDS             1..407
                     /gene="LOC144112922"
                     /coded_by="XM_077645751.1:23..1246"
                     /db_xref="GeneID:144112922"
ORIGIN      
        1 mapstpaqkr pgsrekrrsd adaamdaaak qmeeqvtalv plhsnqvaev taetrsskrk
       61 agessntsgg ikwykqkfrd swlqderfkn wlvsvpddpy rakcrlcnad lragkselek
      121 hartkhhrdt vkavedvkal lgfanqagaq sphdvvtdmq sndqsnnsfq mqddelsrpv
      181 pvasvvtqrr raspvpvptv vsapavrcqv ppmgflprvq chapdfkgia vvdsqireiq
      241 lsdyegkfll llfypqdfsl acpselveys eraaefrnln teilaistds ycthlawtnt
      301 prklgglgkv nvtllsdftk kiskdydvll edigialras fiidpkgmvr qvtvndvnly
      361 rsvdetlrll kalqhvekhg aaicaswepe paggrgcers arsrqdk
//
LOCUS       XP_077501878             376 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112922 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077501878
VERSION     XP_077501878.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645752.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..376
                     /product="uncharacterized protein LOC144112922 isoform X6"
                     /calculated_mol_wt=41875
     Region          192..358
                     /region_name="PRX_Typ2cys"
                     /note="Peroxiredoxin (PRX) family, Typical 2-Cys PRX
                     subfamily; PRXs are thiol-specific antioxidant (TSA)
                     proteins, which confer a protective role in cells through
                     its peroxidase activity by reducing hydrogen peroxide,
                     peroxynitrite, and organic hydroperoxides; cd03015"
                     /db_xref="CDD:239313"
     Site            order(227,261..262,287,289,301)
                     /site_type="other"
                     /note="decamer (pentamer of dimers) interface [polypeptide
                     binding]"
                     /db_xref="CDD:239313"
     Site            order(228,231,307)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:239313"
     Site            order(229..230,233,296,306,319..321,323..325,329..330,337,
                     352..355)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239313"
     Site            order(231,352)
                     /site_type="active"
                     /note="peroxidatic and resolving cysteines [active]"
                     /db_xref="CDD:239313"
     CDS             1..376
                     /gene="LOC144112922"
                     /coded_by="XM_077645752.1:2023..3153"
                     /db_xref="GeneID:144112922"
ORIGIN      
        1 meeqvtalvp lhsnqvaevt aetrsskrka gessntsggi kwykqkfrds wlqderfknw
       61 lvsvpddpyr akcrlcnadl ragkselekh artkhhrdtv kavedvkall gfanqagaqs
      121 phdvvtdmqs ndqsnnsfqm qddelsrpvp vasvvtqrrr aspvpvptvv sapavrcqvp
      181 pmgflprvqc hapdfkgiav vdsqireiql sdyegkflll lfypqdfsla cpselveyse
      241 raaefrnlnt eilaistdsy cthlawtntp rklgglgkvn vtllsdftkk iskdydvlle
      301 digialrasf iidpkgmvrq vtvndvnlyr svdetlrllk alqhvekhga aicaswepep
      361 aggrgcersa rsrqdk
//
LOCUS       XP_077501879             376 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112922 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077501879
VERSION     XP_077501879.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645753.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..376
                     /product="uncharacterized protein LOC144112922 isoform X6"
                     /calculated_mol_wt=41875
     Region          192..358
                     /region_name="PRX_Typ2cys"
                     /note="Peroxiredoxin (PRX) family, Typical 2-Cys PRX
                     subfamily; PRXs are thiol-specific antioxidant (TSA)
                     proteins, which confer a protective role in cells through
                     its peroxidase activity by reducing hydrogen peroxide,
                     peroxynitrite, and organic hydroperoxides; cd03015"
                     /db_xref="CDD:239313"
     Site            order(227,261..262,287,289,301)
                     /site_type="other"
                     /note="decamer (pentamer of dimers) interface [polypeptide
                     binding]"
                     /db_xref="CDD:239313"
     Site            order(228,231,307)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:239313"
     Site            order(229..230,233,296,306,319..321,323..325,329..330,337,
                     352..355)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239313"
     Site            order(231,352)
                     /site_type="active"
                     /note="peroxidatic and resolving cysteines [active]"
                     /db_xref="CDD:239313"
     CDS             1..376
                     /gene="LOC144112922"
                     /coded_by="XM_077645753.1:329..1459"
                     /db_xref="GeneID:144112922"
ORIGIN      
        1 meeqvtalvp lhsnqvaevt aetrsskrka gessntsggi kwykqkfrds wlqderfknw
       61 lvsvpddpyr akcrlcnadl ragkselekh artkhhrdtv kavedvkall gfanqagaqs
      121 phdvvtdmqs ndqsnnsfqm qddelsrpvp vasvvtqrrr aspvpvptvv sapavrcqvp
      181 pmgflprvqc hapdfkgiav vdsqireiql sdyegkflll lfypqdfsla cpselveyse
      241 raaefrnlnt eilaistdsy cthlawtntp rklgglgkvn vtllsdftkk iskdydvlle
      301 digialrasf iidpkgmvrq vtvndvnlyr svdetlrllk alqhvekhga aicaswepep
      361 aggrgcersa rsrqdk
//
LOCUS       XP_077501880             376 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112922 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077501880
VERSION     XP_077501880.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645754.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..376
                     /product="uncharacterized protein LOC144112922 isoform X6"
                     /calculated_mol_wt=41875
     Region          192..358
                     /region_name="PRX_Typ2cys"
                     /note="Peroxiredoxin (PRX) family, Typical 2-Cys PRX
                     subfamily; PRXs are thiol-specific antioxidant (TSA)
                     proteins, which confer a protective role in cells through
                     its peroxidase activity by reducing hydrogen peroxide,
                     peroxynitrite, and organic hydroperoxides; cd03015"
                     /db_xref="CDD:239313"
     Site            order(227,261..262,287,289,301)
                     /site_type="other"
                     /note="decamer (pentamer of dimers) interface [polypeptide
                     binding]"
                     /db_xref="CDD:239313"
     Site            order(228,231,307)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:239313"
     Site            order(229..230,233,296,306,319..321,323..325,329..330,337,
                     352..355)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239313"
     Site            order(231,352)
                     /site_type="active"
                     /note="peroxidatic and resolving cysteines [active]"
                     /db_xref="CDD:239313"
     CDS             1..376
                     /gene="LOC144112922"
                     /coded_by="XM_077645754.1:1899..3029"
                     /db_xref="GeneID:144112922"
ORIGIN      
        1 meeqvtalvp lhsnqvaevt aetrsskrka gessntsggi kwykqkfrds wlqderfknw
       61 lvsvpddpyr akcrlcnadl ragkselekh artkhhrdtv kavedvkall gfanqagaqs
      121 phdvvtdmqs ndqsnnsfqm qddelsrpvp vasvvtqrrr aspvpvptvv sapavrcqvp
      181 pmgflprvqc hapdfkgiav vdsqireiql sdyegkflll lfypqdfsla cpselveyse
      241 raaefrnlnt eilaistdsy cthlawtntp rklgglgkvn vtllsdftkk iskdydvlle
      301 digialrasf iidpkgmvrq vtvndvnlyr svdetlrllk alqhvekhga aicaswepep
      361 aggrgcersa rsrqdk
//
LOCUS       XP_077501882             376 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112922 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077501882
VERSION     XP_077501882.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645756.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..376
                     /product="uncharacterized protein LOC144112922 isoform X6"
                     /calculated_mol_wt=41875
     Region          192..358
                     /region_name="PRX_Typ2cys"
                     /note="Peroxiredoxin (PRX) family, Typical 2-Cys PRX
                     subfamily; PRXs are thiol-specific antioxidant (TSA)
                     proteins, which confer a protective role in cells through
                     its peroxidase activity by reducing hydrogen peroxide,
                     peroxynitrite, and organic hydroperoxides; cd03015"
                     /db_xref="CDD:239313"
     Site            order(227,261..262,287,289,301)
                     /site_type="other"
                     /note="decamer (pentamer of dimers) interface [polypeptide
                     binding]"
                     /db_xref="CDD:239313"
     Site            order(228,231,307)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:239313"
     Site            order(229..230,233,296,306,319..321,323..325,329..330,337,
                     352..355)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239313"
     Site            order(231,352)
                     /site_type="active"
                     /note="peroxidatic and resolving cysteines [active]"
                     /db_xref="CDD:239313"
     CDS             1..376
                     /gene="LOC144112922"
                     /coded_by="XM_077645756.1:204..1334"
                     /db_xref="GeneID:144112922"
ORIGIN      
        1 meeqvtalvp lhsnqvaevt aetrsskrka gessntsggi kwykqkfrds wlqderfknw
       61 lvsvpddpyr akcrlcnadl ragkselekh artkhhrdtv kavedvkall gfanqagaqs
      121 phdvvtdmqs ndqsnnsfqm qddelsrpvp vasvvtqrrr aspvpvptvv sapavrcqvp
      181 pmgflprvqc hapdfkgiav vdsqireiql sdyegkflll lfypqdfsla cpselveyse
      241 raaefrnlnt eilaistdsy cthlawtntp rklgglgkvn vtllsdftkk iskdydvlle
      301 digialrasf iidpkgmvrq vtvndvnlyr svdetlrllk alqhvekhga aicaswepep
      361 aggrgcersa rsrqdk
//
LOCUS       XP_077501883             376 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112922 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077501883
VERSION     XP_077501883.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645757.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..376
                     /product="uncharacterized protein LOC144112922 isoform X6"
                     /calculated_mol_wt=41875
     Region          192..358
                     /region_name="PRX_Typ2cys"
                     /note="Peroxiredoxin (PRX) family, Typical 2-Cys PRX
                     subfamily; PRXs are thiol-specific antioxidant (TSA)
                     proteins, which confer a protective role in cells through
                     its peroxidase activity by reducing hydrogen peroxide,
                     peroxynitrite, and organic hydroperoxides; cd03015"
                     /db_xref="CDD:239313"
     Site            order(227,261..262,287,289,301)
                     /site_type="other"
                     /note="decamer (pentamer of dimers) interface [polypeptide
                     binding]"
                     /db_xref="CDD:239313"
     Site            order(228,231,307)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:239313"
     Site            order(229..230,233,296,306,319..321,323..325,329..330,337,
                     352..355)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239313"
     Site            order(231,352)
                     /site_type="active"
                     /note="peroxidatic and resolving cysteines [active]"
                     /db_xref="CDD:239313"
     CDS             1..376
                     /gene="LOC144112922"
                     /coded_by="XM_077645757.1:260..1390"
                     /db_xref="GeneID:144112922"
ORIGIN      
        1 meeqvtalvp lhsnqvaevt aetrsskrka gessntsggi kwykqkfrds wlqderfknw
       61 lvsvpddpyr akcrlcnadl ragkselekh artkhhrdtv kavedvkall gfanqagaqs
      121 phdvvtdmqs ndqsnnsfqm qddelsrpvp vasvvtqrrr aspvpvptvv sapavrcqvp
      181 pmgflprvqc hapdfkgiav vdsqireiql sdyegkflll lfypqdfsla cpselveyse
      241 raaefrnlnt eilaistdsy cthlawtntp rklgglgkvn vtllsdftkk iskdydvlle
      301 digialrasf iidpkgmvrq vtvndvnlyr svdetlrllk alqhvekhga aicaswepep
      361 aggrgcersa rsrqdk
//
LOCUS       XP_077501884             376 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112922 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077501884
VERSION     XP_077501884.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645758.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..376
                     /product="uncharacterized protein LOC144112922 isoform X6"
                     /calculated_mol_wt=41875
     Region          192..358
                     /region_name="PRX_Typ2cys"
                     /note="Peroxiredoxin (PRX) family, Typical 2-Cys PRX
                     subfamily; PRXs are thiol-specific antioxidant (TSA)
                     proteins, which confer a protective role in cells through
                     its peroxidase activity by reducing hydrogen peroxide,
                     peroxynitrite, and organic hydroperoxides; cd03015"
                     /db_xref="CDD:239313"
     Site            order(227,261..262,287,289,301)
                     /site_type="other"
                     /note="decamer (pentamer of dimers) interface [polypeptide
                     binding]"
                     /db_xref="CDD:239313"
     Site            order(228,231,307)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:239313"
     Site            order(229..230,233,296,306,319..321,323..325,329..330,337,
                     352..355)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239313"
     Site            order(231,352)
                     /site_type="active"
                     /note="peroxidatic and resolving cysteines [active]"
                     /db_xref="CDD:239313"
     CDS             1..376
                     /gene="LOC144112922"
                     /coded_by="XM_077645758.1:264..1394"
                     /db_xref="GeneID:144112922"
ORIGIN      
        1 meeqvtalvp lhsnqvaevt aetrsskrka gessntsggi kwykqkfrds wlqderfknw
       61 lvsvpddpyr akcrlcnadl ragkselekh artkhhrdtv kavedvkall gfanqagaqs
      121 phdvvtdmqs ndqsnnsfqm qddelsrpvp vasvvtqrrr aspvpvptvv sapavrcqvp
      181 pmgflprvqc hapdfkgiav vdsqireiql sdyegkflll lfypqdfsla cpselveyse
      241 raaefrnlnt eilaistdsy cthlawtntp rklgglgkvn vtllsdftkk iskdydvlle
      301 digialrasf iidpkgmvrq vtvndvnlyr svdetlrllk alqhvekhga aicaswepep
      361 aggrgcersa rsrqdk
//
LOCUS       XP_077501885             376 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112922 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077501885
VERSION     XP_077501885.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645759.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..376
                     /product="uncharacterized protein LOC144112922 isoform X6"
                     /calculated_mol_wt=41875
     Region          192..358
                     /region_name="PRX_Typ2cys"
                     /note="Peroxiredoxin (PRX) family, Typical 2-Cys PRX
                     subfamily; PRXs are thiol-specific antioxidant (TSA)
                     proteins, which confer a protective role in cells through
                     its peroxidase activity by reducing hydrogen peroxide,
                     peroxynitrite, and organic hydroperoxides; cd03015"
                     /db_xref="CDD:239313"
     Site            order(227,261..262,287,289,301)
                     /site_type="other"
                     /note="decamer (pentamer of dimers) interface [polypeptide
                     binding]"
                     /db_xref="CDD:239313"
     Site            order(228,231,307)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:239313"
     Site            order(229..230,233,296,306,319..321,323..325,329..330,337,
                     352..355)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239313"
     Site            order(231,352)
                     /site_type="active"
                     /note="peroxidatic and resolving cysteines [active]"
                     /db_xref="CDD:239313"
     CDS             1..376
                     /gene="LOC144112922"
                     /coded_by="XM_077645759.1:102..1232"
                     /db_xref="GeneID:144112922"
ORIGIN      
        1 meeqvtalvp lhsnqvaevt aetrsskrka gessntsggi kwykqkfrds wlqderfknw
       61 lvsvpddpyr akcrlcnadl ragkselekh artkhhrdtv kavedvkall gfanqagaqs
      121 phdvvtdmqs ndqsnnsfqm qddelsrpvp vasvvtqrrr aspvpvptvv sapavrcqvp
      181 pmgflprvqc hapdfkgiav vdsqireiql sdyegkflll lfypqdfsla cpselveyse
      241 raaefrnlnt eilaistdsy cthlawtntp rklgglgkvn vtllsdftkk iskdydvlle
      301 digialrasf iidpkgmvrq vtvndvnlyr svdetlrllk alqhvekhga aicaswepep
      361 aggrgcersa rsrqdk
//
LOCUS       XP_077501886             370 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112922 isoform X7 [Amblyomma
            americanum].
ACCESSION   XP_077501886
VERSION     XP_077501886.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645760.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..370
                     /product="uncharacterized protein LOC144112922 isoform X7"
                     /calculated_mol_wt=41197
     Region          186..352
                     /region_name="PRX_Typ2cys"
                     /note="Peroxiredoxin (PRX) family, Typical 2-Cys PRX
                     subfamily; PRXs are thiol-specific antioxidant (TSA)
                     proteins, which confer a protective role in cells through
                     its peroxidase activity by reducing hydrogen peroxide,
                     peroxynitrite, and organic hydroperoxides; cd03015"
                     /db_xref="CDD:239313"
     Site            order(221,255..256,281,283,295)
                     /site_type="other"
                     /note="decamer (pentamer of dimers) interface [polypeptide
                     binding]"
                     /db_xref="CDD:239313"
     Site            order(222,225,301)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:239313"
     Site            order(223..224,227,290,300,313..315,317..319,323..324,331,
                     346..349)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239313"
     Site            order(225,346)
                     /site_type="active"
                     /note="peroxidatic and resolving cysteines [active]"
                     /db_xref="CDD:239313"
     CDS             1..370
                     /gene="LOC144112922"
                     /coded_by="XM_077645760.1:2024..3136"
                     /db_xref="GeneID:144112922"
ORIGIN      
        1 meeqvtalvp lhsnqvaevt aetrsskrka gessntsggi kwykqkfrds wlqderfknw
       61 lvsvpddpyr akcrlcnadl ragkselekh artkhhrdtv kavedvkall gfanqagaqs
      121 phdvvtdmqs ndqmqddels rpvpvasvvt qrrraspvpv ptvvsapavr cqvppmgflp
      181 rvqchapdfk giavvdsqir eiqlsdyegk fllllfypqd fslacpselv eyseraaefr
      241 nlnteilais tdsycthlaw tntprklggl gkvnvtllsd ftkkiskdyd vlledigial
      301 rasfiidpkg mvrqvtvndv nlyrsvdetl rllkalqhve khgaaicasw epepaggrgc
      361 ersarsrqdk
//
LOCUS       XP_077501887             367 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112922 isoform X8 [Amblyomma
            americanum].
ACCESSION   XP_077501887
VERSION     XP_077501887.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645761.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..367
                     /product="uncharacterized protein LOC144112922 isoform X8"
                     /calculated_mol_wt=41340
     Region          235..>315
                     /region_name="Thioredoxin_like"
                     /note="Protein Disulfide Oxidoreductases and Other
                     Proteins with a Thioredoxin fold; cl00388"
                     /db_xref="CDD:469754"
     CDS             1..367
                     /gene="LOC144112922"
                     /coded_by="XM_077645761.1:143..1246"
                     /db_xref="GeneID:144112922"
ORIGIN      
        1 matverwdvp sgvpgvlygh eafeqcrssk nyaiqqplri yeqmeeqvta lvplhsnqva
       61 evtaetrssk rkagessnts ggikwykqkf rdswlqderf knwlvsvpdd pyrakcrlcn
      121 adlragksel ekhartkhhr dtvkavedvk allgfanqag aqsphdvvtd mqsndqsnns
      181 fqmqddelsr pvpvasvvtq rrraspvpvp tvvsapavrc qvppmgflpr vqchapdfkg
      241 iavvdsqire iqlsdyegkf llllfypqdf slacpselve yseraaefrn lnteilaist
      301 dsycthlawt ntprkvqeee tpekkvcask pgqylglvdi lwrkkkktve swvmsqlgfi
      361 lrvpdsc
//
LOCUS       XP_077501888             365 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112922 isoform X9 [Amblyomma
            americanum].
ACCESSION   XP_077501888
VERSION     XP_077501888.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645762.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..365
                     /product="uncharacterized protein LOC144112922 isoform X9"
                     /calculated_mol_wt=40954
     Region          235..>349
                     /region_name="Thioredoxin_like"
                     /note="Protein Disulfide Oxidoreductases and Other
                     Proteins with a Thioredoxin fold; cl00388"
                     /db_xref="CDD:469754"
     CDS             1..365
                     /gene="LOC144112922"
                     /coded_by="XM_077645762.1:134..1231"
                     /db_xref="GeneID:144112922"
ORIGIN      
        1 matverwdvp sgvpgvlygh eafeqcrssk nyaiqqplri yeqmeeqvta lvplhsnqva
       61 evtaetrssk rkagessnts ggikwykqkf rdswlqderf knwlvsvpdd pyrakcrlcn
      121 adlragksel ekhartkhhr dtvkavedvk allgfanqag aqsphdvvtd mqsndqsnns
      181 fqmqddelsr pvpvasvvtq rrraspvpvp tvvsapavrc qvppmgflpr vqchapdfkg
      241 iavvdsqire iqlsdyegkf llllfypqdf slacpselve yseraaefrn lnteilaist
      301 dsycthlawt ntprklgglg kvnvtllsdf tkkiskdydv lledigials nlcfvgtrtc
      361 wwtrm
//
LOCUS       XP_077501889             142 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: synaptic transmission protein complexin
            [Amblyomma americanum].
ACCESSION   XP_077501889
VERSION     XP_077501889.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645763.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..142
                     /product="LOW QUALITY PROTEIN: synaptic transmission
                     protein complexin"
                     /calculated_mol_wt=16133
     Region          2..133
                     /region_name="Synaphin"
                     /note="Synaphin protein; pfam05835"
                     /db_xref="CDD:461755"
     CDS             1..142
                     /gene="cpx"
                     /coded_by="XM_077645763.1:385..813"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 2 bases in 1 codon"
                     /db_xref="GeneID:144112927"
ORIGIN      
        1 maafvakqmm gsklnavkgq iggdgetsed keaeaeaeqe rlealreaee rrkekhrrme
       61 eerekmrqqi rdkygikkke eekpaevddf aagslnrrkk tpeeiaakte aaeqddltrl
      121 rntietqvne lkssiegkcv lq
//
LOCUS       XP_077501890             296 aa            linear   INV 25-NOV-2025
DEFINITION  somatostatin receptor type 4-like [Amblyomma americanum].
ACCESSION   XP_077501890
VERSION     XP_077501890.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645764.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..296
                     /product="somatostatin receptor type 4-like"
                     /calculated_mol_wt=31882
     Region          22..234
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          23..47
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320331"
     Region          56..78
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320331"
     Region          94..116
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320331"
     Region          132..145
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320331"
     Region          170..192
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320331"
     Region          216..234
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320331"
     CDS             1..296
                     /gene="LOC144112928"
                     /coded_by="XM_077645764.1:189..1079"
                     /db_xref="GeneID:144112928"
ORIGIN      
        1 medswagrql phgpnmggvg plfyvafall lvlgvvgnls vvlmaccyar lrtpaglyav
       61 nvaaadllfc vfgilldpvs vllrrwpfge alcyacsfse hlgvfvhgyt lvvaaarfva
      121 grhprpshls nlgvwflgav vcmphalfvd sralavhgqc vvkwpatvrt pepfvcfaal
      181 aaspfflvtv lyiwlrmspw rgvagrsvvr raslvrvfwl malllllcrt plhvtrclag
      241 vwtalrasah ltvvilatra vaaaavcfta afffdlsakq larksshapp avsnnv
//
LOCUS       XP_077501891             320 aa            linear   INV 25-NOV-2025
DEFINITION  syntaxin 18 [Amblyomma americanum].
ACCESSION   XP_077501891
VERSION     XP_077501891.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645765.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..320
                     /product="syntaxin 18"
                     /calculated_mol_wt=37214
     Region          1..88
                     /region_name="Syntaxin-18_N"
                     /note="SNARE-complex protein Syntaxin-18 N-terminus;
                     pfam10496"
                     /db_xref="CDD:463116"
     Region          231..289
                     /region_name="SNARE"
                     /note="SNARE motif; cl22856"
                     /db_xref="CDD:473982"
     Site            order(259,266,269)
                     /site_type="other"
                     /note="flanking leucine-zipper layers"
                     /db_xref="CDD:277192"
     Site            262
                     /site_type="active"
                     /note="zero layer [active]"
                     /db_xref="CDD:277192"
     CDS             1..320
                     /gene="Syx18"
                     /coded_by="XM_077645765.1:180..1142"
                     /db_xref="GeneID:144112929"
ORIGIN      
        1 mdvtslfkac vktlrtrnka lgmlketdkk cilkprqpps dfgyrvreis sniskmknfl
       61 rehrddyida thvmfstsrm tdaerdridk eaqqfvyqcr evlknlrkev rnqpltpqql
      121 ehhegviyll edhlrsackl yseqkairvk rvydrqklsk lesevkkkap hetegageep
      181 ppvpaeaqps aaaygdvpdl fsfegwdenv visyeefqmf eeenrklyee lnalsdevks
      241 iegrvieisq lqkvftekvl eqdeemerii hklggatenv kdgneqlrqa mrnnagfris
      301 ilffllvmsf sllfldwynp
//
LOCUS       XP_077501892            1189 aa            linear   INV 25-NOV-2025
DEFINITION  sterile20-like kinase [Amblyomma americanum].
ACCESSION   XP_077501892
VERSION     XP_077501892.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645766.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1189
                     /product="sterile20-like kinase"
                     /calculated_mol_wt=136503
     Region          35..314
                     /region_name="STKc_SLK_like"
                     /note="Catalytic domain of Ste20-Like Kinase-like
                     Serine/Threonine Kinases; cd06611"
                     /db_xref="CDD:132942"
     Site            order(47..51,55,68,70,99,115..118,121..122,125,162,
                     164..167,169,179..180,183,197..200,202,234,243)
                     /site_type="active"
                     /db_xref="CDD:132942"
     Site            order(47..51,55,68,70,86,99,113,115..118,121..122,125,
                     166..167,169,179..180)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:132942"
     Site            order(50..51,162,164..166,183,197..200,202,234,243)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:132942"
     Site            179..202
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:132942"
     Region          800..938
                     /region_name="PKK"
                     /note="Polo kinase kinase; pfam12474"
                     /db_xref="CDD:463600"
     Region          968..1111
                     /region_name="PKK"
                     /note="Polo kinase kinase; pfam12474"
                     /db_xref="CDD:463600"
     CDS             1..1189
                     /gene="Slik"
                     /coded_by="XM_077645766.1:395..3964"
                     /db_xref="GeneID:144112930"
ORIGIN      
        1 mssfisnfkk ifnlggvggg dakrrkvcsn irfdenpedy weiigelgdg afgkvykarh
       61 kvtnilaaak icelkgeddl edftveidil veckhqnivd lkeafffegk lwmliefcea
      121 gavdgimidl ekpltepqir ylcheicegl rflhskkvih rdlkagnvll tldgdvkiad
      181 fgvsaknkht lqkrdsfigt pywmapevvl cetyrdnpyd ykadiwslgi tliefaqmep
      241 pnnemnpmrv llkiqksdpp slaqpskwsk efkeflslcl tkdpnqrpts eellkhpfva
      301 ncidrkplkd liseykadvf eevlteeqed eisdyrqsls iendtageti snlsepides
      361 ggetkpvepk gaevraveva kdeevpalpa tkagrlpspp plqaaadkee pvpdkegkal
      421 pkvvaqtapk vpeepvtvqg elpvpeekpk seegtppttl arpaspqtpe aeenlpvpqa
      481 stpirggevv vvsslsmidd stshlkdtse lgtcevdkae lspkapvehe vtfsskhtil
      541 evgeagrweq qagevdrshv svisvgdhev vkdsslvvsp rdspasprlv lrqstagpre
      601 gppekrnsfp cmhegaqqrp plvnggshea lsperpkvrv nvklqqgsas pprvekvsrr
      661 ppastdppve rrrkevsdte svstldsvgs rtsgsdkenr kedweaakek ersansvppi
      721 rkkalmtqnn avrpktlkrt rkfvidgvvm ttttskviyg dldedkqakd yhflrkqelr
      781 elkmlqklen kqfqdldmke efareqqqkk fdqelttllr dydndlealn rtqkqqveka
      841 eqqqdldlky asrriraeqe relktfresl kneakllkqe velmpkekrk dmfrvrkekm
      901 dldqaekerl fveklneqhe lsmkrlsdsh rekiallekq flqqkqqllr areaalwele
      961 erhlhekhql akkqlkdgff lqrhqmlirh ekeleqvkrl nlrredellk rqalerrqlp
     1021 krirsemktr elmfkeslri slhggpdspd edkdrlrkfq eaekkrykae hqrqelkhrr
     1081 qleelkaaad atikeleqlq nekrkmlmeh etqklkqlde ehgtelrewk anlkprkqkl
     1141 eedfarqcee qerfygtstf lndyanmdre qmtlqgpprt ldssyqksy
//
LOCUS       XP_077501893             183 aa            linear   INV 25-NOV-2025
DEFINITION  TM2 domain-containing protein 2 amaretto [Amblyomma americanum].
ACCESSION   XP_077501893
VERSION     XP_077501893.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645767.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="TM2 domain-containing protein 2 amaretto"
                     /calculated_mol_wt=20297
     Region          115..164
                     /region_name="TM2"
                     /note="TM2 domain; pfam05154"
                     /db_xref="CDD:428337"
     CDS             1..183
                     /gene="amrt"
                     /coded_by="XM_077645767.1:71..622"
                     /db_xref="GeneID:144112931"
ORIGIN      
        1 mlwlllfsfl tfstkcvlcv renmhcpsdd cqyrphgplv lcknlpldfl qcsdildlqg
       61 netareelgy gctkfggsky edvqftavnc svlpniecyg nrtflkdgfp cikytghyft
      121 tallysillg flgmdrfclg htgtavgkll tlggvgiwwi vdivllisgn lmpqdgsnwm
      181 ptv
//
LOCUS       XP_077501894            1006 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112932 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501894
VERSION     XP_077501894.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645768.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1006
                     /product="uncharacterized protein LOC144112932 isoform X1"
                     /calculated_mol_wt=112409
     Region          266..381
                     /region_name="TIR_2"
                     /note="TIR domain; pfam13676"
                     /db_xref="CDD:463954"
     CDS             1..1006
                     /gene="LOC144112932"
                     /coded_by="XM_077645768.1:96..3116"
                     /db_xref="GeneID:144112932"
ORIGIN      
        1 mhrpdtlpls hvyqsarlac dsgpsgsasm sgekfpdirv vvgiqyfsdv lpastkcqpt
       61 vlgavvless aisadelrrv mcdqlpslpt hftfitkerw pvqktqehli rvshlmtedr
      121 ivnisrhyek prigvmstsg sslgfvfsdy navlrqfrea iieqlhepkk sgrpefrfve
      181 dngwpvttrq etsltvldvl snrslfiqmg aqptgdvpns imapplgnsl vpatrsptpl
      241 aslpaeewdg krkksvrfgr nepkpilisy vraetaqhal nlkqelhkmn fsvyldidei
      301 kigsdwqdal nyavsechlf vplispmygk tqwtnrevkl advlhkviip vnfletwppe
      361 claiqfastq yiaawkqseh etgqgkpels apagstptge gykydyrvwd etnlkrvake
      421 iaeryrqtvk reqiaerrsk tsslkikrns hlstlatvde msgfrltsmp eaapslcaae
      481 plepaetkel ivitahpkqk dmaqdiarml eqdgfkvwst telnearesc sdtesiptnp
      541 ntprelapip egervfsdty reannrileh krlsslrehe gpgyatysat egrsvcrlts
      601 qasdasvvsm lagekleklt rfqqkavqak vvivlitkef tasrtseqqv fyiehrkhvv
      661 lvkcdegpip cwftslmgnd liardnpefe silririrra tspdtsetpk daaaeatmqc
      721 lvtfikreip sletcvyiag ssklqstrsv eickeigkel akrpnlavvt ggffgapdvv
      781 artfhecrvn ngqtdprvfh ilpkrddkmf tgkvqqnsdg sfaavnygrn lflgnsvker
      841 esavarafdt civveggpca aheveqfvws dhfvvpvvst ggaaggqfgv plkiletphg
      901 iaeekwsvls ekeaspaeva kavveivlql krammshtks hlvrnkykkl kkrnrhsrep
      961 neqqatdath ttdsgpapsl ikptvsfrtm shwqrivnyf rkksts
//
LOCUS       XP_077501895            1026 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112932 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501895
VERSION     XP_077501895.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645769.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1026
                     /product="uncharacterized protein LOC144112932 isoform X2"
                     /calculated_mol_wt=114567
     Region          266..381
                     /region_name="TIR_2"
                     /note="TIR domain; pfam13676"
                     /db_xref="CDD:463954"
     CDS             1..1026
                     /gene="LOC144112932"
                     /coded_by="XM_077645769.1:88..3168"
                     /db_xref="GeneID:144112932"
ORIGIN      
        1 mhrpdtlpls hvyqsarlac dsgpsgsasm sgekfpdirv vvgiqyfsdv lpastkcqpt
       61 vlgavvless aisadelrrv mcdqlpslpt hftfitkerw pvqktqehli rvshlmtedr
      121 ivnisrhyek prigvmstsg sslgfvfsdy navlrqfrea iieqlhepkk sgrpefrfve
      181 dngwpvttrq etsltvldvl snrslfiqmg aqptgdvpns imapplgnsl vpatrsptpl
      241 aslpaeewdg krkksvrfgr nepkpilisy vraetaqhal nlkqelhkmn fsvyldidei
      301 kigsdwqdal nyavsechlf vplispmygk tqwtnrevkl advlhkviip vnfletwppe
      361 claiqfastq yiaawkqseh etgqgkpels apagstptge gykydyrvwd etnlkrvake
      421 iaeryrqtvk reqiaerrsk tsslkikrns hlstlatvde msgfrltsmp eaapslcaae
      481 plepaetkel ivitahpkqk dmaqdiarml eqdgfkvwst telnearesc sdtesiptnp
      541 ntprelapip egervfsdty reannrileh krlsslrehe gpgyatysat egrsvcrlts
      601 qasdasvvsm lagekleklt rfqqkavqak vvivlitkef tasrtseqqv fyiehrkhvv
      661 lvkcdegpip cwftslmgnd liardnpefe silririrra tspdtsetpk daaaeatmqc
      721 lvtfikreip sletcvyiag ssklqstrsv eickeigkel akrpnlavvt ggffgapdvv
      781 artfhecrvn ngqtdprvfh ilpkrddkvg nlllpvfrkl ivaglllqmf tgkvqqnsdg
      841 sfaavnygrn lflgnsvker esavarafdt civveggpca aheveqfvws dhfvvpvvst
      901 ggaaggqfgv plkiletphg iaeekwsvls ekeaspaeva kavveivlql krammshtks
      961 hlvrnkykkl kkrnrhsrep neqqatdath ttdsgpapsl ikptvsfrtm shwqrivnyf
     1021 rkksts
//
LOCUS       XP_077501896             458 aa            linear   INV 25-NOV-2025
DEFINITION  glycerol-3-phosphate acyltransferase 4 [Amblyomma americanum].
ACCESSION   XP_077501896
VERSION     XP_077501896.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645770.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..458
                     /product="glycerol-3-phosphate acyltransferase 4"
                     /calculated_mol_wt=52521
     Region          227..436
                     /region_name="LPLAT_LPCAT1-like"
                     /note="Lysophospholipid Acyltransferases (LPLATs) of
                     Glycerophospholipid Biosynthesis: LPCAT1-like; cd07991"
                     /db_xref="CDD:153253"
     Site            order(257,260,262,276..279,332..334)
                     /site_type="active"
                     /note="putative acyl-acceptor binding pocket [active]"
                     /db_xref="CDD:153253"
     CDS             1..458
                     /gene="Gpat4"
                     /coded_by="XM_077645770.1:93..1469"
                     /db_xref="GeneID:144112933"
ORIGIN      
        1 mamdykshai stllwylshp fqlfvtcass vllllfflsv tntslgirkq yircllklfe
       61 ygrikieran qqrlmredsa edlralnvep tntttsvitr edvivgaeqp iidiplkeyh
      121 lgdityfikk gveaiiddvv tkrfataelp swnlltrtnk nytfvsfrlt viwcigcllr
      181 yvilfpmrva ltcigmiwli cctailgyfp etrfkrflyw hislitfril sravsaivty
      241 hnrenrakpg gicvanhtsp idvamlasdn cyalvgqtqg gfigviqtal nkaashiwfe
      301 raeikdrqav akrlrehved ptklpilifp egtcinntsv mqfkkgsfev ggtiypaaik
      361 ydarfgdafw nsskqnyihy lammmsswai vcdvwylppm hrrddessve fanrvkskia
      421 eqgglldlnw dgllkrsyvk rewkeqqqkd fsqrlklt
//
LOCUS       XP_077501897              85 aa            linear   INV 25-NOV-2025
DEFINITION  ATP synthase, subunit E [Amblyomma americanum].
ACCESSION   XP_077501897
VERSION     XP_077501897.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645771.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..85
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..85
                     /product="ATP synthase, subunit E"
                     /calculated_mol_wt=9644
     Region          5..80
                     /region_name="ATP-synt_E"
                     /note="ATP synthase E chain; pfam05680"
                     /db_xref="CDD:368558"
     CDS             1..85
                     /gene="ATPsynE"
                     /coded_by="XM_077645771.1:114..371"
                     /db_xref="GeneID:144112934"
ORIGIN      
        1 mvelappvsv spfiracrwg flaagiayga fhyrrlsrke akireyeakq mevlkvkrea
       61 ekqkqireem itlakdvgvp vppnf
//
LOCUS       XP_077501898             652 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112935 [Amblyomma americanum].
ACCESSION   XP_077501898
VERSION     XP_077501898.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645772.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..652
                     /product="uncharacterized protein LOC144112935"
                     /calculated_mol_wt=71399
     Region          457..652
                     /region_name="MIEAP"
                     /note="Mitochondria-eating protein; pfam16026"
                     /db_xref="CDD:464982"
     CDS             1..652
                     /gene="LOC144112935"
                     /coded_by="XM_077645772.1:141..2099"
                     /db_xref="GeneID:144112935"
ORIGIN      
        1 mssgrrirpe rpagaqrrnv qkltwrdshw lqgfagdvde prggpsasls dmsggaalrr
       61 vlllcerqqh reaaallsrl apaafralla elpvellldq lpgslpplea lyaraaflee
      121 palqalkpda lllqlvrlfa lqhdadrega pwlgpppmva sckkllkavl kadprarqql
      181 rsrkqaldra leglgqhglv tpaggsaasa geapllhlge alrgqleall srckagalrl
      241 ssgaearvsl srgpapstas hqrqlslrrv qvqerlirnk tlldalepae qaargletll
      301 avlrrriqld kdalcqftql rkeiaedlde aavvapvllr ysagcqrvle lmrdvaeadd
      361 eddsdlsagy hsdsdqsgaa mqragqvcka ssnysssssa vgtdvpssea eelatarqti
      421 salqqrqrel qdrlasqaqr mvgrssrfes taqgdqrpsa lvrrygslya qarvdtldal
      481 daipqlrdad elkskllfsv vvlgfrsvqn tvsdmkaqvr ralqipaqln sdprvielda
      541 svddylranp dacdvsrnve evcaqiwatl ydypclkgca gltryvcdcv rlawalsihg
      601 fvlefetraf rrelhvrfht sdhrserirt ylwpallqap rgpcvhkgvv it
//
LOCUS       XP_077501899             300 aa            linear   INV 25-NOV-2025
DEFINITION  TNF receptor-associated factor 5-like [Amblyomma americanum].
ACCESSION   XP_077501899
VERSION     XP_077501899.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645773.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..300
                     /product="TNF receptor-associated factor 5-like"
                     /calculated_mol_wt=34381
     Region          <64..>140
                     /region_name="COG4372"
                     /note="Uncharacterized protein, contains DUF3084 domain
                     [Function unknown]"
                     /db_xref="CDD:443500"
     Region          148..300
                     /region_name="MATH"
                     /note="MATH (meprin and TRAF-C homology) domain; an
                     independent folding unit with an eight-stranded
                     beta-sandwich structure found in meprins, TRAFs and other
                     proteins. Meprins comprise a class of extracellular
                     metalloproteases which are anchored to the membrane...;
                     cl02446"
                     /db_xref="CDD:445786"
     Site            order(206,267..269)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:238068"
     CDS             1..300
                     /gene="LOC144112936"
                     /coded_by="XM_077645773.1:204..1106"
                     /db_xref="GeneID:144112936"
ORIGIN      
        1 megdpaetnl mdspvqvvhr hqcspgrrtc rlsvlgctye gtqedlerhe tleshmnfil
       61 tytekangsm etlrqaltes tqqnlelqss lnaikeqmtd mlreqqnlqe qlliltsrvh
      121 dgqeqcqrma egvdsrleem lsrswsqgkf vwyikpysvl rrqqengeia rvvsapfytg
      181 vpgyklrlma dlngygegrg shlslflqvm qgkfdcvldw pckyehvlrv vdqtgrglhv
      241 drqqsfrsip srskhlmgrp vhecnvpigf htmaplselh nersgfvrnd tlvivyrcri
//
LOCUS       XP_077501900             227 aa            linear   INV 25-NOV-2025
DEFINITION  putative caffeoyl-CoA O-methyltransferase 2 [Amblyomma americanum].
ACCESSION   XP_077501900
VERSION     XP_077501900.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645774.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..227
                     /product="putative caffeoyl-CoA O-methyltransferase 2"
                     /calculated_mol_wt=25198
     Region          30..227
                     /region_name="AdoMet_MTases"
                     /note="S-adenosylmethionine-dependent methyltransferases
                     (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes
                     that use S-adenosyl-L-methionine (SAM or AdoMet) as a
                     substrate for methyltransfer, creating the product
                     S-adenosyl-L-homocysteine (AdoHcy); cl17173"
                     /db_xref="CDD:473071"
     Site            order(74..75,78..82,99..100,126..128)
                     /site_type="other"
                     /note="S-adenosylmethionine binding site [chemical
                     binding]"
                     /db_xref="CDD:100107"
     CDS             1..227
                     /gene="LOC144112938"
                     /coded_by="XM_077645774.1:264..947"
                     /db_xref="GeneID:144112938"
ORIGIN      
        1 mdnapsrpgr ekgkittpea eayarehcvl hpvlqkliae ttklphgrmy tkppqlqlyq
       61 illkamrakk ylevgvftgc salsaalalp pdgtvrgldi nretadfgrp fwteagveqk
      121 idirigkaie sldaliadge sesydfayid adkegyddyy erclrlvkpn gviafdntlr
      181 ggrvfddqat dpeallmrtl naklkrderv qlsmlpfgdg vnivlkl
//
LOCUS       XP_077501901             227 aa            linear   INV 25-NOV-2025
DEFINITION  putative caffeoyl-CoA O-methyltransferase 2 [Amblyomma americanum].
ACCESSION   XP_077501901
VERSION     XP_077501901.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645775.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..227
                     /product="putative caffeoyl-CoA O-methyltransferase 2"
                     /calculated_mol_wt=25198
     Region          30..227
                     /region_name="AdoMet_MTases"
                     /note="S-adenosylmethionine-dependent methyltransferases
                     (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes
                     that use S-adenosyl-L-methionine (SAM or AdoMet) as a
                     substrate for methyltransfer, creating the product
                     S-adenosyl-L-homocysteine (AdoHcy); cl17173"
                     /db_xref="CDD:473071"
     Site            order(74..75,78..82,99..100,126..128)
                     /site_type="other"
                     /note="S-adenosylmethionine binding site [chemical
                     binding]"
                     /db_xref="CDD:100107"
     CDS             1..227
                     /gene="LOC144112938"
                     /coded_by="XM_077645775.1:96..779"
                     /db_xref="GeneID:144112938"
ORIGIN      
        1 mdnapsrpgr ekgkittpea eayarehcvl hpvlqkliae ttklphgrmy tkppqlqlyq
       61 illkamrakk ylevgvftgc salsaalalp pdgtvrgldi nretadfgrp fwteagveqk
      121 idirigkaie sldaliadge sesydfayid adkegyddyy erclrlvkpn gviafdntlr
      181 ggrvfddqat dpeallmrtl naklkrderv qlsmlpfgdg vnivlkl
//
LOCUS       XP_077501902             204 aa            linear   INV 25-NOV-2025
DEFINITION  preli-like [Amblyomma americanum].
ACCESSION   XP_077501902
VERSION     XP_077501902.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645776.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..204
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..204
                     /product="preli-like"
                     /calculated_mol_wt=23277
     Region          16..166
                     /region_name="PRELI"
                     /note="PRELI-like family; pfam04707"
                     /db_xref="CDD:398400"
     CDS             1..204
                     /gene="prel"
                     /coded_by="XM_077645776.1:134..748"
                     /db_xref="GeneID:144112939"
ORIGIN      
        1 mvkfyerkdr fkfswdqvaq gfwarypnpy sthvltediv aryveggklf tkrlltktnp
       61 mnrlpkwger fvssksvriv eesvvdprtr tlvtytrnig lqhimsieek ciyrpaadnp
      121 khtvverkaw vsssifgfsc aiqafgverf kqnaakackg ftyvlermfp gthpaekehl
      181 rsklatelak akavplvaaa rcsn
//
LOCUS       XP_077501904             760 aa            linear   INV 25-NOV-2025
DEFINITION  WD repeat-containing protein 70-like [Amblyomma americanum].
ACCESSION   XP_077501904
VERSION     XP_077501904.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645778.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..760
                     /product="WD repeat-containing protein 70-like"
                     /calculated_mol_wt=83038
     Region          <273..579
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          296..338
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          344..392
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          397..438
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          447..485
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          493..531
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          539..579
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..760
                     /gene="LOC144112940"
                     /coded_by="XM_077645778.1:20..2302"
                     /db_xref="GeneID:144112940"
ORIGIN      
        1 maassegrgi vspnsakkar vfdvnaifee aritavsrsk garmaedpmp stsdpesnve
       61 dkasrdntcv ssvtrskdds erlvgppvnl saepnstkka rvfdlnaife earitavsrs
      121 kgariaedpm pstsdpesnv edkasrdstc vcsitrskdd serlvgppvn lsaernstkk
      181 arvfdlnaif eearrtamsr skearlaedp kpstsdpesn vkdgatggss stsaatrsee
      241 gsdedlvgpp itlcaevnee gtsseedeeg sehtteayrt ipvkeqltlr hgtkivsala
      301 ldpngarlvt ggydfditlf dfagmdsslq pfrslrpcdc hqirnleysc tgdtflvvsg
      361 saqakvldrd gfevmecvkg dqyitdmart kghvamlnyg cwhprsredf ltcandgtlr
      421 lwnvdkpnch kalvktkqlg glraipstcr fsrdgqlmva gcqdgslqfw dqrrtlvhps
      481 smvreahqrg tditcmafah dgrqlatrsc ddtlklwdtr alrptalhtf aeldnlypat
      541 dcgfspddrv vytatsnaag vgqlvfferd tmrevlrlql pspvariiwh arlnqllagl
      601 acgevrllyd aayshhgall caskaararp tpqgltlaqv ltphalplfr eerprssrkr
      661 lekarkdpvl sqrpdlpvtg pgqggriasa gntlssyivr nlgttkrvdd nvdpreailk
      721 yakeaaenpy wvtpayastq pkpvfqesee ptpakkpkqa
//
LOCUS       XP_077501905             628 aa            linear   INV 25-NOV-2025
DEFINITION  sec1 family domain containing Slh [Amblyomma americanum].
ACCESSION   XP_077501905
VERSION     XP_077501905.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645779.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..628
                     /product="sec1 family domain containing Slh"
                     /calculated_mol_wt=70631
     Region          34..619
                     /region_name="Sec1"
                     /note="Sec1 family; pfam00995"
                     /db_xref="CDD:460020"
     CDS             1..628
                     /gene="Slh"
                     /coded_by="XM_077645779.1:236..2122"
                     /db_xref="GeneID:144112941"
ORIGIN      
        1 mslnirdkqi aalkqmlnfn vpqpkgsfse pvwkllvydr cgqdiispll svkelrdmgi
       61 tlhmllhsdr dpipevpaiy fvaptdenit risqdfrnel ydqyylnfvs pvsrqhledl
      121 asaalqansv anvskvfdqy lnfitlendl fllkhndrht vsyyainrgd vkdteiesim
      181 dnivdclfsv fatlgtvpii rspkgnaaem vaekldkrir dnlrdsrnsl fldsthgggq
      241 fsfqrpllvv ldrnmdmatp lhhtwtyqal ahdvlglsln rvtleepvap spssehvgak
      301 prkktktfdl tqadkfwqqh kgspfptvae avqeeleayr aqedevkklk aamglegdrt
      361 deaitmlsdn takltsavss lpellerkrl idmhtsiata ilehikarkl dlyfeteekl
      421 lgrqaldrsl ldlindpeag taqdklrlil iafvlgldga leyeealqra gcdlsalryl
      481 rrwkdytrit apqpmgsygg gprtvgmfsk lmsqgsqfvm egvknlvvkk hklpitrlvd
      541 almelkslpe tddfryldpk llgraepraq apfqeavvfv lgggnyieyq nlldytkgks
      601 kkvvygctql vnaaqfleql sqlgaeik
//
LOCUS       XP_077501906             256 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112942 [Amblyomma americanum].
ACCESSION   XP_077501906
VERSION     XP_077501906.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645780.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..256
                     /product="uncharacterized protein LOC144112942"
                     /calculated_mol_wt=28701
     Region          1..>246
                     /region_name="transpos_IS630"
                     /note="IS630 family transposase; NF033545"
                     /db_xref="CDD:468076"
     CDS             1..256
                     /gene="LOC144112942"
                     /coded_by="XM_077645780.1:208..978"
                     /db_xref="GeneID:144112942"
ORIGIN      
        1 mtgvsprtvv rfkkeglsgg vkspqkrpkr maisssrsvk ydsftvnair lklhrmyaer
       61 qiptldsilh evngdddlpd mskatlwrll kemgfkfekr krtlaliers divawrrryl
      121 raikafrsqg rcivyldetw vnaghtrqhv wkdstvkssq daflrglttg lpapsgkggr
      181 lilvhagssl tgfvsgaadf frakkgasad yhsemdgkyf eewfsskllp nipnssiivm
      241 dnapyhsval ekpptt
//
LOCUS       XP_077501907             620 aa            linear   INV 25-NOV-2025
DEFINITION  RING finger protein 145-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501907
VERSION     XP_077501907.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645781.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..620
                     /product="RING finger protein 145-like isoform X1"
                     /calculated_mol_wt=69652
     Region          31..516
                     /region_name="TRC8_N"
                     /note="TRC8 N-terminal domain; pfam13705"
                     /db_xref="CDD:463960"
     Region          545..585
                     /region_name="RING-H2_RNF139-like"
                     /note="RING finger, H2 subclass, found in RING finger
                     proteins RNF139, RNF145, and similar proteins; cd16476"
                     /db_xref="CDD:438139"
     CDS             1..620
                     /gene="LOC144112943"
                     /coded_by="XM_077645781.1:134..1996"
                     /db_xref="GeneID:144112943"
ORIGIN      
        1 mkcvslgcvp vrsfcinais fmmrpsraqv daavgiimrv pglfiidyww qhdrnksvpq
       61 smslpgvlna altnlvlvhg flllllpipr vrclytnfvs allllcshll skyyiqmets
      121 lsrhldvdes farrqltafl ahivlacivf allegrsrpm lpilscytlp vlartldfpp
      181 eslevlhnfg nalmcvsvac ylysqvpsli sflkdtyvdt lvlamrfgwv gllilfwhkl
      241 fvpthflvfw liafcvklae syptwespwy llalssasnv csspvtlvas svtvsylayl
      301 tlsgtkaflh gsltflndnp mhsgwtegit mlllalqtgl teikmpsrva vlliilfivm
      361 ssmlqsvlei tepvvlalsa sqsrqlgrhv rtlgmctflv afplhitwrl sslfpidfwm
      421 mivlsscilt svqvvgllvi yglfvydawr tepwealddv iyaaravtki leflvalfvv
      481 gmgfwesttg kwnwanasil lvhcyfnvwq rmqagwksfl lrreavrkte slpeatseql
      541 llhndvcsic ysemrsacvt kcqhffhrtc lrkwlyiqdk cplchahvsv enqqnpgpsc
      601 npepaaaaaa dedtnqevts
//
LOCUS       XP_077501908             599 aa            linear   INV 25-NOV-2025
DEFINITION  RING finger protein 145-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077501908
VERSION     XP_077501908.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645782.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..599
                     /product="RING finger protein 145-like isoform X2"
                     /calculated_mol_wt=67395
     Region          10..495
                     /region_name="TRC8_N"
                     /note="TRC8 N-terminal domain; pfam13705"
                     /db_xref="CDD:463960"
     Region          524..564
                     /region_name="RING-H2_RNF139-like"
                     /note="RING finger, H2 subclass, found in RING finger
                     proteins RNF139, RNF145, and similar proteins; cd16476"
                     /db_xref="CDD:438139"
     CDS             1..599
                     /gene="LOC144112943"
                     /coded_by="XM_077645782.1:148..1947"
                     /db_xref="GeneID:144112943"
ORIGIN      
        1 mmrpsraqvd aavgiimrvp glfiidywwq hdrnksvpqs mslpgvlnaa ltnlvlvhgf
       61 lllllpiprv rclytnfvsa llllcshlls kyyiqmetsl srhldvdesf arrqltafla
      121 hivlacivfa llegrsrpml pilscytlpv lartldfppe slevlhnfgn almcvsvacy
      181 lysqvpslis flkdtyvdtl vlamrfgwvg llilfwhklf vpthflvfwl iafcvklaes
      241 yptwespwyl lalssasnvc sspvtlvass vtvsylaylt lsgtkaflhg sltflndnpm
      301 hsgwtegitm lllalqtglt eikmpsrvav lliilfivms smlqsvleit epvvlalsas
      361 qsrqlgrhvr tlgmctflva fplhitwrls slfpidfwmm ivlsscilts vqvvgllviy
      421 glfvydawrt epwealddvi yaaravtkil eflvalfvvg mgfwesttgk wnwanasill
      481 vhcyfnvwqr mqagwksfll rreavrktes lpeatseqll lhndvcsicy semrsacvtk
      541 cqhffhrtcl rkwlyiqdkc plchahvsve nqqnpgpscn pepaaaaaad edtnqevts
//
LOCUS       XP_077501909             599 aa            linear   INV 25-NOV-2025
DEFINITION  RING finger protein 145-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077501909
VERSION     XP_077501909.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645783.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..599
                     /product="RING finger protein 145-like isoform X2"
                     /calculated_mol_wt=67395
     Region          10..495
                     /region_name="TRC8_N"
                     /note="TRC8 N-terminal domain; pfam13705"
                     /db_xref="CDD:463960"
     Region          524..564
                     /region_name="RING-H2_RNF139-like"
                     /note="RING finger, H2 subclass, found in RING finger
                     proteins RNF139, RNF145, and similar proteins; cd16476"
                     /db_xref="CDD:438139"
     CDS             1..599
                     /gene="LOC144112943"
                     /coded_by="XM_077645783.1:82..1881"
                     /db_xref="GeneID:144112943"
ORIGIN      
        1 mmrpsraqvd aavgiimrvp glfiidywwq hdrnksvpqs mslpgvlnaa ltnlvlvhgf
       61 lllllpiprv rclytnfvsa llllcshlls kyyiqmetsl srhldvdesf arrqltafla
      121 hivlacivfa llegrsrpml pilscytlpv lartldfppe slevlhnfgn almcvsvacy
      181 lysqvpslis flkdtyvdtl vlamrfgwvg llilfwhklf vpthflvfwl iafcvklaes
      241 yptwespwyl lalssasnvc sspvtlvass vtvsylaylt lsgtkaflhg sltflndnpm
      301 hsgwtegitm lllalqtglt eikmpsrvav lliilfivms smlqsvleit epvvlalsas
      361 qsrqlgrhvr tlgmctflva fplhitwrls slfpidfwmm ivlsscilts vqvvgllviy
      421 glfvydawrt epwealddvi yaaravtkil eflvalfvvg mgfwesttgk wnwanasill
      481 vhcyfnvwqr mqagwksfll rreavrktes lpeatseqll lhndvcsicy semrsacvtk
      541 cqhffhrtcl rkwlyiqdkc plchahvsve nqqnpgpscn pepaaaaaad edtnqevts
//
LOCUS       XP_077501910             599 aa            linear   INV 25-NOV-2025
DEFINITION  RING finger protein 145-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077501910
VERSION     XP_077501910.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645784.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..599
                     /product="RING finger protein 145-like isoform X2"
                     /calculated_mol_wt=67395
     Region          10..495
                     /region_name="TRC8_N"
                     /note="TRC8 N-terminal domain; pfam13705"
                     /db_xref="CDD:463960"
     Region          524..564
                     /region_name="RING-H2_RNF139-like"
                     /note="RING finger, H2 subclass, found in RING finger
                     proteins RNF139, RNF145, and similar proteins; cd16476"
                     /db_xref="CDD:438139"
     CDS             1..599
                     /gene="LOC144112943"
                     /coded_by="XM_077645784.1:192..1991"
                     /db_xref="GeneID:144112943"
ORIGIN      
        1 mmrpsraqvd aavgiimrvp glfiidywwq hdrnksvpqs mslpgvlnaa ltnlvlvhgf
       61 lllllpiprv rclytnfvsa llllcshlls kyyiqmetsl srhldvdesf arrqltafla
      121 hivlacivfa llegrsrpml pilscytlpv lartldfppe slevlhnfgn almcvsvacy
      181 lysqvpslis flkdtyvdtl vlamrfgwvg llilfwhklf vpthflvfwl iafcvklaes
      241 yptwespwyl lalssasnvc sspvtlvass vtvsylaylt lsgtkaflhg sltflndnpm
      301 hsgwtegitm lllalqtglt eikmpsrvav lliilfivms smlqsvleit epvvlalsas
      361 qsrqlgrhvr tlgmctflva fplhitwrls slfpidfwmm ivlsscilts vqvvgllviy
      421 glfvydawrt epwealddvi yaaravtkil eflvalfvvg mgfwesttgk wnwanasill
      481 vhcyfnvwqr mqagwksfll rreavrktes lpeatseqll lhndvcsicy semrsacvtk
      541 cqhffhrtcl rkwlyiqdkc plchahvsve nqqnpgpscn pepaaaaaad edtnqevts
//
LOCUS       XP_077501911            1822 aa            linear   INV 25-NOV-2025
DEFINITION  ribosomal RNA Processing 5 [Amblyomma americanum].
ACCESSION   XP_077501911
VERSION     XP_077501911.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645785.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1822
                     /product="ribosomal RNA Processing 5"
                     /calculated_mol_wt=200933
     Region          70..169
                     /region_name="S1_Rrp5_repeat_hs1_sc1"
                     /note="Rrp5 is a trans-acting factor important for
                     biogenesis of both the 40S and 60S eukaryotic ribosomal
                     subunits. Rrp5 has two distinct regions, an N-terminal
                     region containing tandemly repeated S1 RNA-binding domains
                     (12 S1 repeats in Saccharomyces...; cd05693"
                     /db_xref="CDD:240198"
     Site            order(81,89,99,101)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:240198"
     Region          177..255
                     /region_name="S1_like"
                     /note="Ribosomal protein S1-like RNA-binding domain. Found
                     in a wide variety of RNA-associated proteins. Originally
                     identified in S1 ribosomal protein. This superfamily also
                     contains the Cold Shock Domain (CSD), which is a homolog
                     of the S1 domain. Both domains...; cl09927"
                     /db_xref="CDD:471952"
     Site            order(188,196,207,209)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:238152"
     Region          <374..783
                     /region_name="rpsA"
                     /note="ribosomal protein S1; TIGR00717"
                     /db_xref="CDD:273232"
     Region          1111..1170
                     /region_name="S1_like"
                     /note="Ribosomal protein S1-like RNA-binding domain. Found
                     in a wide variety of RNA-associated proteins. Originally
                     identified in S1 ribosomal protein. This superfamily also
                     contains the Cold Shock Domain (CSD), which is a homolog
                     of the S1 domain. Both domains...; cl09927"
                     /db_xref="CDD:471952"
     Site            order(1114,1122,1132,1134)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:238094"
     Region          1200..1271
                     /region_name="S1_like"
                     /note="Ribosomal protein S1-like RNA-binding domain. Found
                     in a wide variety of RNA-associated proteins. Originally
                     identified in S1 ribosomal protein. This superfamily also
                     contains the Cold Shock Domain (CSD), which is a homolog
                     of the S1 domain. Both domains...; cl09927"
                     /db_xref="CDD:471952"
     Site            order(1208,1216,1226,1228)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:238094"
     Region          1275..1350
                     /region_name="S1_like"
                     /note="Ribosomal protein S1-like RNA-binding domain. Found
                     in a wide variety of RNA-associated proteins. Originally
                     identified in S1 ribosomal protein. This superfamily also
                     contains the Cold Shock Domain (CSD), which is a homolog
                     of the S1 domain. Both domains...; cl09927"
                     /db_xref="CDD:471952"
     Site            order(1286,1295,1305,1307)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:238094"
     Region          <1363..1484
                     /region_name="PLN00207"
                     /note="polyribonucleotide nucleotidyltransferase;
                     Provisional"
                     /db_xref="CDD:215104"
     Region          1375..1433
                     /region_name="S1"
                     /note="Ribosomal protein S1-like RNA-binding domain;
                     smart00316"
                     /db_xref="CDD:197648"
     Site            order(1384,1392,1402,1404)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:238094"
     Region          1615..1812
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Region          1615..1638
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(1617..1618,1621..1622,1624,1644,1647..1648,
                     1651..1652,1654..1655,1678,1681..1682,1685..1686,1689)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          1642..1672
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          1677..1705
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          1713..1736
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          1747..1773
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     CDS             1..1822
                     /gene="Rrp5"
                     /coded_by="XM_077645785.1:108..5576"
                     /db_xref="GeneID:144112944"
ORIGIN      
        1 meeilprgev rrgpssvkks nlekddlfka rdsglpkkrs rkrpkksqel esaedigssv
       61 vvepltirll aeglvllgrv qqvqdfglrv slpgaitgrv altnisqpys lllrqfaqgv
      121 qeetmeilpl pklfqegqtv vckvisaapv ewgsskvsvn lsldpsavna smtpstlqkg
      181 mvlqaavasi edhgytmdcg vegvnaflsr aeaaafikkc naghalavgq liqcavladt
      241 rggravqlta rpstvnapve lqdfsldhvl pgfraeltvi esqeeglvvs whgieacvhr
      301 shlpgpwdkp edivvgetmv gtllyvqplv qrpylsiqap lgrvpfgtvr pgalleyaaq
      361 vvaveagavh lrlepsgvra lcprplvsdq dvedardfit agqclscrvm alsymdrvvv
      421 vslkksmlse kyvapealvp gakfqctvrq vqasgvlvsl spwlagfiqa lhlsdrkgek
      481 pavgsslrcr llhvdrssdp prllltcrra lvtsrrpiit syddaapgrv tdglivrvtp
      541 kgllvtlynn ikgwipplei pnpvhgiean yapgkivtcr vidcqpseer ltlslkltak
      601 geedkeesve pvkrslkpga pevgmivsgs iearvddgft vllrtgeras lprrhlsdte
      661 shcsrlqrfl pdrcslanlv vfnsaehlvv srkaslvnaa hrglfvsgie nmepgtlvfg
      721 vllsfvrhgm llelpagirg fvplkdvade flsdasksgf vigqclvakv tkvnsetrqv
      781 wlstsmrncg gemgirgtme slaaqlgdee llsrleqsvv psvssivrvt vqyvdeedkv
      841 ihcliadssl lataatvpgr sitsgsthea vvlccspeqs alelsldpqv vrsintgrkr
      901 kpvqrriqpg rvllvmhshv vvllhsghlc lvpakshlns tfcqpfvkga tvsvwtwkvc
      961 gnvsigctka tadrlkhmea adfisqeeka alasnvvvqp srhdlqaagv slvsperngs
     1021 qsveqcviga qpckrkglqs etisekdale ackyvqsyds saeapaledd lenaaesasr
     1081 geittisqkp grgtseqsys rvkeemqtyr avvrskeklq lkvtletgck grvhisfvde
     1141 qpkegesplk rfepgdeikv yvlkrviplh sralaitgrr nfcecslfpa qctdfapapg
     1201 sevfgyfshf segnlfftlt hnkmgklpvl nmdlpparla yiqrdfragq amklkvlrad
     1261 essmvelsql dvttlavdsv vvgcivntrp gvgacvslpl ghhgtisitd vcddfaqtaa
     1321 lmedyerery lqcyildvds etstyhlstr esrlhpesgr evvdkevval snlsvgstlr
     1381 gfvktinkhg cfvtvgcgvd glipmsklpa llqrnhkair vgelvdvvvr riqaserwlf
     1441 ldlvdcgqvk akatrkrkls tssmdepifd ssekrarldp lprlsldegf swdvqatpdl
     1501 atllnerpsl vssddeddgs karkshkeir qereqaeael rkkermlvdp srepetvddf
     1561 drlvlvspns sivwlrymaf hlrqaeieka ravarralas iefreeqekl nvwtallnle
     1621 hlygtqdsld svfkealqfn dplkiymhla qiyvagnkqe qaeqlykqml nkfkqhadvw
     1681 lsfglfymkf nqaeacrall qralkslpsr ehvgiiskfa qmefkhgeae rgrsmfisil
     1741 dnypkrtdlw lvyvdmlikh cdldnarraf ekatslnlnp kkmkslfkkw ldfekqygda
     1801 asckevkqra vqyveqrtsv sq
//
LOCUS       XP_077501912            1822 aa            linear   INV 25-NOV-2025
DEFINITION  ribosomal RNA Processing 5 [Amblyomma americanum].
ACCESSION   XP_077501912
VERSION     XP_077501912.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645786.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1822
                     /product="ribosomal RNA Processing 5"
                     /calculated_mol_wt=200933
     Region          70..169
                     /region_name="S1_Rrp5_repeat_hs1_sc1"
                     /note="Rrp5 is a trans-acting factor important for
                     biogenesis of both the 40S and 60S eukaryotic ribosomal
                     subunits. Rrp5 has two distinct regions, an N-terminal
                     region containing tandemly repeated S1 RNA-binding domains
                     (12 S1 repeats in Saccharomyces...; cd05693"
                     /db_xref="CDD:240198"
     Site            order(81,89,99,101)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:240198"
     Region          177..255
                     /region_name="S1_like"
                     /note="Ribosomal protein S1-like RNA-binding domain. Found
                     in a wide variety of RNA-associated proteins. Originally
                     identified in S1 ribosomal protein. This superfamily also
                     contains the Cold Shock Domain (CSD), which is a homolog
                     of the S1 domain. Both domains...; cl09927"
                     /db_xref="CDD:471952"
     Site            order(188,196,207,209)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:238152"
     Region          <374..783
                     /region_name="rpsA"
                     /note="ribosomal protein S1; TIGR00717"
                     /db_xref="CDD:273232"
     Region          1111..1170
                     /region_name="S1_like"
                     /note="Ribosomal protein S1-like RNA-binding domain. Found
                     in a wide variety of RNA-associated proteins. Originally
                     identified in S1 ribosomal protein. This superfamily also
                     contains the Cold Shock Domain (CSD), which is a homolog
                     of the S1 domain. Both domains...; cl09927"
                     /db_xref="CDD:471952"
     Site            order(1114,1122,1132,1134)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:238094"
     Region          1200..1271
                     /region_name="S1_like"
                     /note="Ribosomal protein S1-like RNA-binding domain. Found
                     in a wide variety of RNA-associated proteins. Originally
                     identified in S1 ribosomal protein. This superfamily also
                     contains the Cold Shock Domain (CSD), which is a homolog
                     of the S1 domain. Both domains...; cl09927"
                     /db_xref="CDD:471952"
     Site            order(1208,1216,1226,1228)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:238094"
     Region          1275..1350
                     /region_name="S1_like"
                     /note="Ribosomal protein S1-like RNA-binding domain. Found
                     in a wide variety of RNA-associated proteins. Originally
                     identified in S1 ribosomal protein. This superfamily also
                     contains the Cold Shock Domain (CSD), which is a homolog
                     of the S1 domain. Both domains...; cl09927"
                     /db_xref="CDD:471952"
     Site            order(1286,1295,1305,1307)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:238094"
     Region          <1363..1484
                     /region_name="PLN00207"
                     /note="polyribonucleotide nucleotidyltransferase;
                     Provisional"
                     /db_xref="CDD:215104"
     Region          1375..1433
                     /region_name="S1"
                     /note="Ribosomal protein S1-like RNA-binding domain;
                     smart00316"
                     /db_xref="CDD:197648"
     Site            order(1384,1392,1402,1404)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:238094"
     Region          1615..1812
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Region          1615..1638
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(1617..1618,1621..1622,1624,1644,1647..1648,
                     1651..1652,1654..1655,1678,1681..1682,1685..1686,1689)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          1642..1672
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          1677..1705
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          1713..1736
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          1747..1773
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     CDS             1..1822
                     /gene="Rrp5"
                     /coded_by="XM_077645786.1:125..5593"
                     /db_xref="GeneID:144112944"
ORIGIN      
        1 meeilprgev rrgpssvkks nlekddlfka rdsglpkkrs rkrpkksqel esaedigssv
       61 vvepltirll aeglvllgrv qqvqdfglrv slpgaitgrv altnisqpys lllrqfaqgv
      121 qeetmeilpl pklfqegqtv vckvisaapv ewgsskvsvn lsldpsavna smtpstlqkg
      181 mvlqaavasi edhgytmdcg vegvnaflsr aeaaafikkc naghalavgq liqcavladt
      241 rggravqlta rpstvnapve lqdfsldhvl pgfraeltvi esqeeglvvs whgieacvhr
      301 shlpgpwdkp edivvgetmv gtllyvqplv qrpylsiqap lgrvpfgtvr pgalleyaaq
      361 vvaveagavh lrlepsgvra lcprplvsdq dvedardfit agqclscrvm alsymdrvvv
      421 vslkksmlse kyvapealvp gakfqctvrq vqasgvlvsl spwlagfiqa lhlsdrkgek
      481 pavgsslrcr llhvdrssdp prllltcrra lvtsrrpiit syddaapgrv tdglivrvtp
      541 kgllvtlynn ikgwipplei pnpvhgiean yapgkivtcr vidcqpseer ltlslkltak
      601 geedkeesve pvkrslkpga pevgmivsgs iearvddgft vllrtgeras lprrhlsdte
      661 shcsrlqrfl pdrcslanlv vfnsaehlvv srkaslvnaa hrglfvsgie nmepgtlvfg
      721 vllsfvrhgm llelpagirg fvplkdvade flsdasksgf vigqclvakv tkvnsetrqv
      781 wlstsmrncg gemgirgtme slaaqlgdee llsrleqsvv psvssivrvt vqyvdeedkv
      841 ihcliadssl lataatvpgr sitsgsthea vvlccspeqs alelsldpqv vrsintgrkr
      901 kpvqrriqpg rvllvmhshv vvllhsghlc lvpakshlns tfcqpfvkga tvsvwtwkvc
      961 gnvsigctka tadrlkhmea adfisqeeka alasnvvvqp srhdlqaagv slvsperngs
     1021 qsveqcviga qpckrkglqs etisekdale ackyvqsyds saeapaledd lenaaesasr
     1081 geittisqkp grgtseqsys rvkeemqtyr avvrskeklq lkvtletgck grvhisfvde
     1141 qpkegesplk rfepgdeikv yvlkrviplh sralaitgrr nfcecslfpa qctdfapapg
     1201 sevfgyfshf segnlfftlt hnkmgklpvl nmdlpparla yiqrdfragq amklkvlrad
     1261 essmvelsql dvttlavdsv vvgcivntrp gvgacvslpl ghhgtisitd vcddfaqtaa
     1321 lmedyerery lqcyildvds etstyhlstr esrlhpesgr evvdkevval snlsvgstlr
     1381 gfvktinkhg cfvtvgcgvd glipmsklpa llqrnhkair vgelvdvvvr riqaserwlf
     1441 ldlvdcgqvk akatrkrkls tssmdepifd ssekrarldp lprlsldegf swdvqatpdl
     1501 atllnerpsl vssddeddgs karkshkeir qereqaeael rkkermlvdp srepetvddf
     1561 drlvlvspns sivwlrymaf hlrqaeieka ravarralas iefreeqekl nvwtallnle
     1621 hlygtqdsld svfkealqfn dplkiymhla qiyvagnkqe qaeqlykqml nkfkqhadvw
     1681 lsfglfymkf nqaeacrall qralkslpsr ehvgiiskfa qmefkhgeae rgrsmfisil
     1741 dnypkrtdlw lvyvdmlikh cdldnarraf ekatslnlnp kkmkslfkkw ldfekqygda
     1801 asckevkqra vqyveqrtsv sq
//
LOCUS       XP_077501913             905 aa            linear   INV 25-NOV-2025
DEFINITION  catenin alpha isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501913
VERSION     XP_077501913.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645787.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..905
                     /product="catenin alpha isoform X1"
                     /calculated_mol_wt=100792
     Region          19..865
                     /region_name="Vinculin"
                     /note="Vinculin family; pfam01044"
                     /db_xref="CDD:460040"
     CDS             1..905
                     /gene="alpha-Cat"
                     /coded_by="XM_077645787.1:161..2878"
                     /db_xref="GeneID:144112946"
ORIGIN      
        1 mstmepppva lkwdpknlei ktlsvektle plviqvttlv ntkgpskkkk grskrahvlv
       61 aaveratenf ikkgeeiate npdihqemma avdevrktge amskasrefa edpcssvkrg
      121 nmvrsarnll savtrllila dmvdvhrllk slrvveddld kvknassqse lmeffrnfgv
      181 ntveliqqaa rrqaelkdsr lrddlaaara vlkknsmmll taskvyirhp elsaakenrd
      241 fvfrqvceav ntigdvaqgr agalvpsyeg pgelaaaldd fdervvldpl tynelrtrpa
      301 leerlesiis gaalmadssc trderreriv aecnavrqal qdllaeymas agrkedsldk
      361 aveqmgrktr dlrrqlrkav vdhvsdsfle tqvpllvlve aaragderqv eeyarvfaeh
      421 ahklvevasl acsmsshedg vkmvrcaaah ieglcpqvvn aarilaarpr skvaqenmda
      481 frdawetqvr llteavddit tiddflavse nhiledvnkc vlalqendad aldrtagair
      541 grsarvcnvv tsemdnyepg iytervleav avlrdqvmpn faqkvemave alsaspqkei
      601 denefidasr lvydgvreir ravlmnrsae eldseteiey edntyetrsk ssahtadyde
      661 ypdisginnt rdayrlvsee ekqkiaaqve tfrteknkfd revakwddtg ndiivlakqm
      721 cmimmemtdf trgkgplktt mdvinaakki seygtkldkl artiadqcpe sstkkdliay
      781 lqrialychq lnitskvkad vqnisgnliv sgldsatsli qaaknlmnav vltvkasyva
      841 stkyprsgvv sspivvwkmk apekkplvrr ekpeevrakv rkgsqkknia pikalsefqs
      901 laesv
//
LOCUS       XP_077501914             905 aa            linear   INV 25-NOV-2025
DEFINITION  catenin alpha isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501914
VERSION     XP_077501914.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645788.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..905
                     /product="catenin alpha isoform X1"
                     /calculated_mol_wt=100792
     Region          19..865
                     /region_name="Vinculin"
                     /note="Vinculin family; pfam01044"
                     /db_xref="CDD:460040"
     CDS             1..905
                     /gene="alpha-Cat"
                     /coded_by="XM_077645788.1:280..2997"
                     /db_xref="GeneID:144112946"
ORIGIN      
        1 mstmepppva lkwdpknlei ktlsvektle plviqvttlv ntkgpskkkk grskrahvlv
       61 aaveratenf ikkgeeiate npdihqemma avdevrktge amskasrefa edpcssvkrg
      121 nmvrsarnll savtrllila dmvdvhrllk slrvveddld kvknassqse lmeffrnfgv
      181 ntveliqqaa rrqaelkdsr lrddlaaara vlkknsmmll taskvyirhp elsaakenrd
      241 fvfrqvceav ntigdvaqgr agalvpsyeg pgelaaaldd fdervvldpl tynelrtrpa
      301 leerlesiis gaalmadssc trderreriv aecnavrqal qdllaeymas agrkedsldk
      361 aveqmgrktr dlrrqlrkav vdhvsdsfle tqvpllvlve aaragderqv eeyarvfaeh
      421 ahklvevasl acsmsshedg vkmvrcaaah ieglcpqvvn aarilaarpr skvaqenmda
      481 frdawetqvr llteavddit tiddflavse nhiledvnkc vlalqendad aldrtagair
      541 grsarvcnvv tsemdnyepg iytervleav avlrdqvmpn faqkvemave alsaspqkei
      601 denefidasr lvydgvreir ravlmnrsae eldseteiey edntyetrsk ssahtadyde
      661 ypdisginnt rdayrlvsee ekqkiaaqve tfrteknkfd revakwddtg ndiivlakqm
      721 cmimmemtdf trgkgplktt mdvinaakki seygtkldkl artiadqcpe sstkkdliay
      781 lqrialychq lnitskvkad vqnisgnliv sgldsatsli qaaknlmnav vltvkasyva
      841 stkyprsgvv sspivvwkmk apekkplvrr ekpeevrakv rkgsqkknia pikalsefqs
      901 laesv
//
LOCUS       XP_077501915             896 aa            linear   INV 25-NOV-2025
DEFINITION  catenin alpha isoform X2 [Amblyomma americanum].
ACCESSION   XP_077501915
VERSION     XP_077501915.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645789.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..896
                     /product="catenin alpha isoform X2"
                     /calculated_mol_wt=99815
     Region          19..865
                     /region_name="Vinculin"
                     /note="Vinculin family; pfam01044"
                     /db_xref="CDD:460040"
     CDS             1..896
                     /gene="alpha-Cat"
                     /coded_by="XM_077645789.1:280..2970"
                     /db_xref="GeneID:144112946"
ORIGIN      
        1 mstmepppva lkwdpknlei ktlsvektle plviqvttlv ntkgpskkkk grskrahvlv
       61 aaveratenf ikkgeeiate npdihqemma avdevrktge amskasrefa edpcssvkrg
      121 nmvrsarnll savtrllila dmvdvhrllk slrvveddld kvknassqse lmeffrnfgv
      181 ntveliqqaa rrqaelkdsr lrddlaaara vlkknsmmll taskvyirhp elsaakenrd
      241 fvfrqvceav ntigdvaqgr agalvpsyeg pgelaaaldd fdervvldpl tynelrtrpa
      301 leerlesiis gaalmadssc trderreriv aecnavrqal qdllaeymas agrkedsldk
      361 aveqmgrktr dlrrqlrkav vdhvsdsfle tqvpllvlve aaragderqv eeyarvfaeh
      421 ahklvevasl acsmsshedg vkmvrcaaah ieglcpqvvn aarilaarpr skvaqenmda
      481 frdawetqvr llteavddit tiddflavse nhiledvnkc vlalqendad aldrtagair
      541 grsarvcnvv tsemdnyepg iytervleav avlrdqvmpn faqkvemave alsaspqkei
      601 denefidasr lvydgvreir ravlmnrsae eldseteiey edntyetrsk ssahtadyde
      661 ypdisginnt rdayrlvsee ekqkiaaqve tfrteknkfd revakwddtg ndiivlakqm
      721 cmimmemtdf trgkgplktt mdvinaakki seygtkldkl artiadqcpe sstkkdliay
      781 lqrialychq lnitskvkad vqnisgnliv sgldsatsli qaaknlmnav vltvkasyva
      841 stkyprsgvv sspivvwkmk apekkplvrr ekpeevrakv rkgsqkknia pikalt
//
LOCUS       XP_077501916             896 aa            linear   INV 25-NOV-2025
DEFINITION  catenin alpha isoform X2 [Amblyomma americanum].
ACCESSION   XP_077501916
VERSION     XP_077501916.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645790.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..896
                     /product="catenin alpha isoform X2"
                     /calculated_mol_wt=99815
     Region          19..865
                     /region_name="Vinculin"
                     /note="Vinculin family; pfam01044"
                     /db_xref="CDD:460040"
     CDS             1..896
                     /gene="alpha-Cat"
                     /coded_by="XM_077645790.1:159..2849"
                     /db_xref="GeneID:144112946"
ORIGIN      
        1 mstmepppva lkwdpknlei ktlsvektle plviqvttlv ntkgpskkkk grskrahvlv
       61 aaveratenf ikkgeeiate npdihqemma avdevrktge amskasrefa edpcssvkrg
      121 nmvrsarnll savtrllila dmvdvhrllk slrvveddld kvknassqse lmeffrnfgv
      181 ntveliqqaa rrqaelkdsr lrddlaaara vlkknsmmll taskvyirhp elsaakenrd
      241 fvfrqvceav ntigdvaqgr agalvpsyeg pgelaaaldd fdervvldpl tynelrtrpa
      301 leerlesiis gaalmadssc trderreriv aecnavrqal qdllaeymas agrkedsldk
      361 aveqmgrktr dlrrqlrkav vdhvsdsfle tqvpllvlve aaragderqv eeyarvfaeh
      421 ahklvevasl acsmsshedg vkmvrcaaah ieglcpqvvn aarilaarpr skvaqenmda
      481 frdawetqvr llteavddit tiddflavse nhiledvnkc vlalqendad aldrtagair
      541 grsarvcnvv tsemdnyepg iytervleav avlrdqvmpn faqkvemave alsaspqkei
      601 denefidasr lvydgvreir ravlmnrsae eldseteiey edntyetrsk ssahtadyde
      661 ypdisginnt rdayrlvsee ekqkiaaqve tfrteknkfd revakwddtg ndiivlakqm
      721 cmimmemtdf trgkgplktt mdvinaakki seygtkldkl artiadqcpe sstkkdliay
      781 lqrialychq lnitskvkad vqnisgnliv sgldsatsli qaaknlmnav vltvkasyva
      841 stkyprsgvv sspivvwkmk apekkplvrr ekpeevrakv rkgsqkknia pikalt
//
LOCUS       XP_077501917             282 aa            linear   INV 25-NOV-2025
DEFINITION  RNA-binding motif protein 13 [Amblyomma americanum].
ACCESSION   XP_077501917
VERSION     XP_077501917.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645791.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..282
                     /product="RNA-binding motif protein 13"
                     /calculated_mol_wt=33216
     Region          1..274
                     /region_name="PLN00040"
                     /note="Protein MAK16 homolog; Provisional"
                     /db_xref="CDD:215038"
     CDS             1..282
                     /gene="Rbm13"
                     /coded_by="XM_077645791.1:128..976"
                     /db_xref="GeneID:144112947"
ORIGIN      
        1 mqnddviwsi ikgsfcsfkv ntkgqqfcrn dfnltglcnr sscplansfy atikeeegic
       61 ylymktiera afpdrlwekv klsknfekal eqinqnlvyw prfvrqkckq rllkihqyii
      121 rmrklrlsrq rklvpmqrki errerrreek alvaarldta iekelldrlr qgtykdiynf
      181 pqkafdnalk seeveseves deeeeeeeed ehdvgaveyv aaddmeesdl sdledlgees
      241 sseeeeadre kivatpakrk rphveieyek evepstskin vk
//
LOCUS       XP_077501918             499 aa            linear   INV 25-NOV-2025
DEFINITION  V-type proton ATPase subunit Vha55 [Amblyomma americanum].
ACCESSION   XP_077501918
VERSION     XP_077501918.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645792.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..499
                     /product="V-type proton ATPase subunit Vha55"
                     /calculated_mol_wt=55017
     Region          30..492
                     /region_name="V-ATPase_V1_B"
                     /note="V-type (H+)-ATPase V1, B subunit; TIGR01040"
                     /db_xref="CDD:273410"
     CDS             1..499
                     /gene="Vha55"
                     /coded_by="XM_077645792.1:130..1629"
                     /db_xref="GeneID:144112948"
ORIGIN      
        1 mpdpksavaa srehalavsr dyisqprlvy ktvcgvngpl vildevkfpk yaeivqlvla
       61 dgtartgqvl evsgskavvq vfegtsgida kntvceftgd ilripvsedm lgrvfngsgk
      121 pidkgppvla edfldiqgqp inpwsriype emiqtgisai dvmnsiargq kipifsaagl
      181 phndiaaqic rqgglvklpg ksvldssedn faivfaamgv nmetarffkq dfeengsmen
      241 vclflnland ptieriitpr lalttaefla yqcekhvlvi ltdmssyaea lrevsaaree
      301 vpgrrgfpgy mytdlatiye ragrvegrng sitqipiltm pnddithpip dltgyitegq
      361 vyvdrqlhnr qvyppinvlp slsrlmksai gegmtrkdha dvsnqlyacy aigkdvqamk
      421 avvgeealga edllyleflt kfeknfisqg syenrtifes ldigwqllri fpkemlkrip
      481 qsllqefypr dgrqgaeak
//
LOCUS       XP_077501919             310 aa            linear   INV 25-NOV-2025
DEFINITION  enoyl-CoA hydratase domain-containing protein 3, mitochondrial-like
            [Amblyomma americanum].
ACCESSION   XP_077501919
VERSION     XP_077501919.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645793.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..310
                     /product="enoyl-CoA hydratase domain-containing protein 3,
                     mitochondrial-like"
                     /calculated_mol_wt=33828
     Region          45..303
                     /region_name="crotonase-like"
                     /note="Crotonase/Enoyl-Coenzyme A (CoA) hydratase
                     superfamily. This superfamily contains a diverse set of
                     enzymes including enoyl-CoA hydratase, napthoate synthase,
                     methylmalonyl-CoA decarboxylase, 3-hydoxybutyryl-CoA
                     dehydratase, and dienoyl-CoA isomerase; cl23717"
                     /db_xref="CDD:474030"
     Site            order(71,73,105,109..113,154,156..158,180..181,184)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:119339"
     Site            order(111,158)
                     /site_type="active"
                     /note="oxyanion hole (OAH) forming residues [active]"
                     /db_xref="CDD:119339"
     Site            order(138,146,167..170,182..185,191,193..194,196..197,
                     202..203,205..206,208..209,212,223,226,241,244..245)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:119339"
     CDS             1..310
                     /gene="LOC144112949"
                     /coded_by="XM_077645793.1:133..1065"
                     /db_xref="GeneID:144112949"
ORIGIN      
        1 mmptfsclsi rtcgtmrycl prlfskmkre itrtsltkqv hstallrsad lvsvhdaggv
       61 rkitlnnpkk rnvlslamlr eldaqfkktd vessvrcvvl cssgpvfssg hdlkelkids
      121 sdtakiqeif slctrvmtsi rrlsvpvvaq vnglaaaagc qlvatcdivl atdkssfslp
      181 gaafglfcst pgiavarcvp qktsaymlmt gnainaqeal asglvskvvp eeklqeetdr
      241 vtsaivaksr svlalgkkfy yrqiqmgied ayaegeqvml hnlqycdsqe ginafaekrk
      301 phwehtdrkv
//
LOCUS       XP_077501920             294 aa            linear   INV 25-NOV-2025
DEFINITION  protein phosphatase 1 regulatory subunit 3B-B-like [Amblyomma
            americanum].
ACCESSION   XP_077501920
VERSION     XP_077501920.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645794.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..294
                     /product="protein phosphatase 1 regulatory subunit
                     3B-B-like"
                     /calculated_mol_wt=33263
     Region          73..103
                     /region_name="PBD_PPP1R3"
                     /note="PP1C binding domain found in protein phosphatase 1
                     regulatory subunit 3 (PPP1R3) family; cl41685"
                     /db_xref="CDD:455118"
     Site            order(80..86,88,90,92..98)
                     /site_type="other"
                     /note="PP1C binding site [polypeptide binding]"
                     /db_xref="CDD:439278"
     Region          145..251
                     /region_name="CBM_21"
                     /note="Carbohydrate/starch-binding module (family 21);
                     pfam03370"
                     /db_xref="CDD:427265"
     CDS             1..294
                     /gene="LOC144112950"
                     /coded_by="XM_077645794.1:301..1185"
                     /db_xref="GeneID:144112950"
ORIGIN      
        1 mpmdlelvls anpplfvgse crlsvcalqq gnplfqtcsv krnyrrastn vppikpcmsp
       61 rdeqvqsept sptssnrikr rvsfaddrgy sltqvrvmke psdspprwtd efvaritrdm
      121 rldaplwett ftqpasdyle frqtldanlv slenvivksd elitgtikvk nvtfekqvlv
      181 ratfdgwktm qdyaaeyvpn ghlsnmydtf cfsipipssa sgnlgviefc vcfkhdqqeh
      241 wdnnngknyk lvarakkaps ptvvrrftda lkadvdswte faswknlase gpyw
//
LOCUS       XP_077501921             294 aa            linear   INV 25-NOV-2025
DEFINITION  protein phosphatase 1 regulatory subunit 3B-B-like [Amblyomma
            americanum].
ACCESSION   XP_077501921
VERSION     XP_077501921.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645795.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..294
                     /product="protein phosphatase 1 regulatory subunit
                     3B-B-like"
                     /calculated_mol_wt=33263
     Region          73..103
                     /region_name="PBD_PPP1R3"
                     /note="PP1C binding domain found in protein phosphatase 1
                     regulatory subunit 3 (PPP1R3) family; cl41685"
                     /db_xref="CDD:455118"
     Site            order(80..86,88,90,92..98)
                     /site_type="other"
                     /note="PP1C binding site [polypeptide binding]"
                     /db_xref="CDD:439278"
     Region          145..251
                     /region_name="CBM_21"
                     /note="Carbohydrate/starch-binding module (family 21);
                     pfam03370"
                     /db_xref="CDD:427265"
     CDS             1..294
                     /gene="LOC144112950"
                     /coded_by="XM_077645795.1:216..1100"
                     /db_xref="GeneID:144112950"
ORIGIN      
        1 mpmdlelvls anpplfvgse crlsvcalqq gnplfqtcsv krnyrrastn vppikpcmsp
       61 rdeqvqsept sptssnrikr rvsfaddrgy sltqvrvmke psdspprwtd efvaritrdm
      121 rldaplwett ftqpasdyle frqtldanlv slenvivksd elitgtikvk nvtfekqvlv
      181 ratfdgwktm qdyaaeyvpn ghlsnmydtf cfsipipssa sgnlgviefc vcfkhdqqeh
      241 wdnnngknyk lvarakkaps ptvvrrftda lkadvdswte faswknlase gpyw
//
LOCUS       XP_077501922             228 aa            linear   INV 25-NOV-2025
DEFINITION  DNA replication complex GINS protein Sld5 [Amblyomma americanum].
ACCESSION   XP_077501922
VERSION     XP_077501922.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645796.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..228
                     /product="DNA replication complex GINS protein Sld5"
                     /calculated_mol_wt=25896
     Region          30..150
                     /region_name="GINS_A_Sld5"
                     /note="Alpha-helical domain of GINS complex protein Sld5;
                     cd11711"
                     /db_xref="CDD:212549"
     Site            order(56,59,97,101,105..106,126,129,131,133..143)
                     /site_type="other"
                     /note="tetramer interface [polypeptide binding]"
                     /db_xref="CDD:212549"
     Region          169..228
                     /region_name="GINS_B_Sld5"
                     /note="beta-strand (B) domain of GINS complex protein
                     Sld5; cd21692"
                     /db_xref="CDD:412028"
     Site            order(170,172..174,176,199,204,207..212)
                     /site_type="other"
                     /note="tetramer interface [polypeptide binding]"
                     /db_xref="CDD:412028"
     CDS             1..228
                     /gene="Sld5"
                     /coded_by="XM_077645796.1:125..811"
                     /db_xref="GeneID:144112951"
ORIGIN      
        1 mdsepdvegi ankygyaesh eeectsaeqv vhkleeawln esfspellpy qndivesvld
       61 qlnymkenlq gvgkngfrat mhkmeveriq yvlssylkir lgkiekhgaf vleqqergei
      121 dsddllslee rrfarayvas iedylhdval vhmpfnqqdf smasvdagpe ldeyvflkvk
      181 rqtlgvlvde etsqsrdgev idldegsqhi iryrviaplv kdgsvvll
//
LOCUS       XP_077501923              54 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112952 [Amblyomma americanum].
ACCESSION   XP_077501923
VERSION     XP_077501923.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645797.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..54
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..54
                     /product="uncharacterized protein LOC144112952"
                     /calculated_mol_wt=5837
     Region          1..43
                     /region_name="ATP_synth_reg"
                     /note="ATP synthase regulation; pfam14960"
                     /db_xref="CDD:434349"
     CDS             1..54
                     /gene="LOC144112952"
                     /coded_by="XM_077645797.1:164..328"
                     /db_xref="GeneID:144112952"
ORIGIN      
        1 magvdesqfk glskyfntft nrgrsniaks tylvlgtigf lywakkkasg ksak
//
LOCUS       XP_077501924            3483 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112954 [Amblyomma americanum].
ACCESSION   XP_077501924
VERSION     XP_077501924.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645798.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..3483
                     /product="uncharacterized protein LOC144112954"
                     /calculated_mol_wt=388808
     Region          <163..285
                     /region_name="COG1340"
                     /note="Uncharacterized coiled-coil protein, contains
                     DUF342 domain [Function unknown]"
                     /db_xref="CDD:440951"
     Region          1099..>1234
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          1964..>2418
                     /region_name="Med15"
                     /note="ARC105 or Med15 subunit of Mediator complex
                     non-fungal; pfam09606"
                     /db_xref="CDD:312941"
     Region          2889..3480
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..3483
                     /gene="LOC144112954"
                     /coded_by="XM_077645798.1:94..10545"
                     /db_xref="GeneID:144112954"
ORIGIN      
        1 maappggspr apppgfppgp pglppggspg lppggsprpg fplrpggtpp rtgltpprpp
       61 ggsplrpgfp ptgltpprtp gggpvpvlpp pppglpppgl ppppglpppp gepgapppeg
      121 appvegappa dasapppppp pppaapapap ppppppaasa dnerdvlvkk skeelqqlin
      181 kkleawdklt lskeneerie alrklmtink develagkvg pkdklmevmd trlklaketl
      241 dqinaeikke tadrlktldk weqgaiqdir klnrmesela mrlakrgrrp mgrssmdqmd
      301 epdmedmegd mdrdmdrgrd rdwnrdrgrd rdrgrdrdrd ydrdrgrdrd rdydrdrdrd
      361 rdrdrdrdrd rdrdrdrdrd gdrdrdrdrd rdrdrdrgrd rdrdrdrdrd rdrgrddrgr
      421 dkdrdsdtdk drgkgkdkdk dkekedakek akaekeaqek ekkemeekve akqtkvqrvs
      481 mieletgdsq vcddnllklh elefriandm lkgkykgrkg reltskelke klrrlcdlev
      541 qiadelfgqe seasgtvevd gtitkatikr spergqrrds rdtddrrdre rygdhdsdrr
      601 dfsdrrdyyd rreyspeymd qyagqmdynp qtaefradyr qmttqmapqm qpipqmqpmp
      661 amqpmpqmqp mpqpmpqpma qpapqpvmpq pmaqtvlqrt gpgptlgaie eigndlvrhq
      721 enievsgesg vqvikhtths elircgsaep hamelaskpa fsmesidpgr kpivatfeqv
      781 addqttvvkl ktaidieiip qgtnkplsma ekteaatrsk peteedeppp pptplkkkrs
      841 teaqrarspt tlrspsppti eeedddaldd eeeplapqvp elgdrrvaad deippgpsfw
      901 qwicqsiysp rggactvtae rteraapqgr grsrsrdrdd rvrekeeelr qlidsvikvs
      961 qdvilarrai elggvgedrc vgnmleaegk lrnlidlemr lanelsnfrn sdmvaneesr
     1021 isvlnaeeki rqlidvesrl aegfvaerks svkskisdam segamrnfaf eetahgtevr
     1081 gmprnvpkkp rkllphmlpd sspgrkskat dnesrkgrrk kcadadeeye peerrssrpa
     1141 rtrsrrraee ddsrpprrkg rdrgdderpa rrkgsrrgqq degdeddvsr rrargrrsks
     1201 hkaddgeddd ssrarrgspl lrgrrdqspk krgsptrgpq rqlirvqegv pvkislsgkm
     1261 legkkrrsks genftdmasa pemtrkagvs ttevsssisi sddvteattt vvrksssdgt
     1321 iigkrsisfg tprnrpakar tpssgarsgk gssqisvlti slsdsdevap qrspssqqkr
     1381 arhimaarsr pespqwtppr ptqhvtrscs rhlamqkaqf rsprgysrsp spkpgypvaa
     1441 aaaaaarmys aspqrvrspv rggsprrtvf evvdetayya epvvrdrarl mrredqrfqm
     1501 sadepktqre gkwhepklrv syasrvpshg thstsslsvs sttalnyerg ddpprhhrit
     1561 sqldrycepp rvasydssrr qasssrpysy vspmlayllp srrargvssq grdtsvsras
     1621 rgsppradhe gspgrllfrv sssrmarsas lprdspnass prlspslrtp kistsrdvrt
     1681 pqsprrsrsl hhrrqdlspa rlklcvasss rlgndtpssq lesppipsrg tspewlerdf
     1741 fprgplfppp pkppvpppmp ppkgfsmtpl glestttsas airitmsppp mppvppmlpp
     1801 ptpteiaalr aatvtmadmr resssseley gaeevahqvy eyrqlqferl fhpdfnalqg
     1861 vddqrqqlqq qlqelervlq qqdgapvlqv qfqspvdpsg kpstplaqsv lqvqfqgpsq
     1921 pvpqvfqaqi ptraespgvi qvqlhspavp seprrtests isrttqpeqp gvlqiqlqsp
     1981 ldlpstqgaa qpdqlsvlqa qiqsaadfpt lskspqsdqp gaiqlpgpse pqstiadqsa
     2041 lfqmqmqgla qmpqldqlaq qkfqsqlgfp mqetgqvqqp elpgvlqmqf hspstasmgf
     2101 vnqqtaqaid ieqsllrssm aqmefsgqsm pqghvswssa phvglpnqtl sqvqqsmqqa
     2161 gqleqpmlhs smqqmgmsgh pvsfgqqadq hflemqfqst lmpstdfptr plpqppfqcm
     2221 pmdqggaqam flenqqeqfq rqiqlgvssm mtyphgpgqa tqssvhqlqp qgppmqemrd
     2281 wahqsmqgep vqrmqdwgsl mhmpaqalqm sepmsgpair dgtcqamgql rlfdqplpsr
     2341 lpfsprrqpp spsmqmmmqa dllgsrsspq vhlpgsrmps aemrfrdmrq eqllrqksqp
     2401 htlvmvqrsd rdirrpiper mprprssepr mprpprsgtd dfamrpppwr tripmpprgm
     2461 spssrpemhp pvpppprsfp pprmvarmpp pdlvkkepep akkppqgdap passqesspa
     2521 ssqqqtqkpp eakpqqseqq kpqpqqqqvp qqreqqqrrl asqasepcmq qiplpvtgye
     2581 pttpgppfpr fpfrvprprs atplrsfspl pprpmspsqp rlpdfrqlel qtklqklile
     2641 rqqqqmqeqm kaaarlqssp rtprspgspv qilvrsspnl plygqqqmll qqrmqeqynm
     2701 hrmqqanqmn rlrnmtsatd gggfmrrkml satkssmpdt dsasdygngt mdsksvaals
     2761 nmiearkppl sphahahaht qrkqnyrhsa ietrmrnsqt emvvyfvvll atiiglwtvl
     2821 diygrrtvvh slngsyiavp natmrgfrds sgqsgipanv sgqptfelcs sapclaegsy
     2881 vshqlnwnvn pcesmyefvc snsppkpade lllsdlelsl lrqlrgspat gspaarvmqd
     2941 lwkecldqet rnrlgwgplk hmfkatslsd wpylqfttqg eravwqaaar alryfglatl
     3001 vslepsehpl lgrspllald kpvlllrpgn dsswyyeavs lalssvahkn iakhplaldv
     3061 tnfarqlasv vrspgasgdl grdaklerlr svpryrpllv gflqndtlvs ddtelllkap
     3121 eyairlgyll ddtaphvaln yigfrllvhv spflpesvrk lvplrarqlg lgdhgpgqlv
     3181 clravehalp tlfyraayes hrplldalla adlggtlkqa lasrlallhw ldgdtrdral
     3241 qrlhqleprl lfpswvtdad kaeqdvrqap hviageglrs yaritkhlmq rrlhsadpwl
     3301 gqapsddqcw lgpgrrvlyv pltalnasap aqepfvllql arlgerasrc lvrllldgag
     3361 nraedpraww tpaarqglae lrkcytlqsr gerptaaees aalvvahaaf earmpaldfr
     3421 lenavsmsld qlffihytlg ycaadsdvar rvnlallnfg gfqraftcdv gtpmnprksc
     3481 dfw
//
LOCUS       XP_077501925             226 aa            linear   INV 25-NOV-2025
DEFINITION  upstream activation factor subunit spp27 homolog Non2 [Amblyomma
            americanum].
ACCESSION   XP_077501925
VERSION     XP_077501925.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645799.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..226
                     /product="upstream activation factor subunit spp27 homolog
                     Non2"
                     /calculated_mol_wt=25329
     Region          7..57
                     /region_name="DEK_C"
                     /note="DEK C terminal domain; pfam08766"
                     /db_xref="CDD:462592"
     Region          152..222
                     /region_name="SWIB-MDM2_like"
                     /note="SWIB/MDM2 domain found in SWIB/MDM2 homologous
                     proteins; cd10567"
                     /db_xref="CDD:349489"
     Site            order(170,173..174,176..177,180..181,186,191..192,194,
                     214..215)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349489"
     CDS             1..226
                     /gene="Non2"
                     /coded_by="XM_077645799.1:53..733"
                     /db_xref="GeneID:144112955"
ORIGIN      
        1 maevpvselr ksisemlega dlsalsskkv rkqleekygi dftdrkkeid nlvmelitpt
       61 ekkeepkgne eqngagssas ptgsssseme dddeerndgd deelarklqd eevrsrsrav
      121 kksrpakkep akakkatkss gtkresaysr kcalspelaa vvgaeqmars dvvkkmwsiv
      181 rernlfdpsn rqfalcdpql lkvfgqkrvr mfgmmkylkn hikdvk
//
LOCUS       XP_077501926             585 aa            linear   INV 25-NOV-2025
DEFINITION  R3H and coiled-coil domain-containing protein 1-like [Amblyomma
            americanum].
ACCESSION   XP_077501926
VERSION     XP_077501926.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645800.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..585
                     /product="R3H and coiled-coil domain-containing protein
                     1-like"
                     /calculated_mol_wt=64122
     Region          18..80
                     /region_name="R3H"
                     /note="R3H domain. The name of the R3H domain comes from
                     the characteristic spacing of the most conserved arginine
                     and histidine residues. R3H domains are found in proteins
                     together with ATPase domains, SF1 helicase domains, SF2
                     DEAH helicase domains, Cys-rich...; cl00297"
                     /db_xref="CDD:469711"
     Site            order(48,52)
                     /site_type="other"
                     /note="RxxxH motif"
                     /db_xref="CDD:100067"
     Region          441..502
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..585
                     /gene="LOC144112956"
                     /coded_by="XM_077645800.1:79..1836"
                     /db_xref="GeneID:144112956"
ORIGIN      
        1 mdgladyqrg gerdgvfvkt vfeeitnfvq advrnsvlly pvlpkhqrfl thkvveenfe
       61 dlcsfslgkg elrrtavclk srlifafdsg ycldeavrkv avrlcrekgl pvdffshslw
      121 rpppaskkme apasrpaskk meapaprpar rqrrpdmqly vprgrkaeal ktaasadslq
      181 kqmaasqplp eshaaattad tlghhpnera gifkedksse hgcaaeistq llqaadvcgr
      241 ergakctaly vdsssdvlst sansknsaav vkvglplsqe ndtvnneemm henaeevhsl
      301 peplgatakc eglqekedls sledgagvfd dsnvgiseqs ecvtlekpfs vlqlgatqlr
      361 sakadtsgdc aslaetstgd kstdaedswd affdesgeci dpevlknitk algnikvhqa
      421 kldyskfetr itdlseaeyg hileiynfpa efktkdlvta lssskdqfsi rwvddthala
      481 ifsspfaate alglqnplmk lrhvseaskq sklkikrcse llmpykprpq tsasvarrlv
      541 sgalgmrvnv daeqrkkerq vlleakgkrr taakqrvdaw ngdvs
//
LOCUS       XP_077501928             415 aa            linear   INV 25-NOV-2025
DEFINITION  palmitoyltransferase ZDHHC16 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501928
VERSION     XP_077501928.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645802.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..415
                     /product="palmitoyltransferase ZDHHC16 isoform X1"
                     /calculated_mol_wt=47467
     Region          142..>212
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; pfam01529"
                     /db_xref="CDD:396215"
     CDS             1..415
                     /gene="LOC144112957"
                     /coded_by="XM_077645802.1:288..1535"
                     /db_xref="GeneID:144112957"
ORIGIN      
        1 mdivmrfiry irrgferlky rvrhkflypr ivfnslvyne fadiyyvgdt cmeplfwivd
       61 hftkamgpif vatvtivact viaiayligi pywwennkyv laialvighw lllnivfhyw
      121 malttnpgtp peasmvpevv sickkciapk pprahhcsvc nrcilkmdhh cpwlnncigh
      181 fthryffmfc syvllgiiyl mifgykiaye eyfgtllaps vglansmiql gnldmsanvt
      241 lhdipgipgs lrtapgavvl qlnnvpltyd grcchrwglc dpgcssraas cyqsaamnnr
      301 tdaakqdfvr kyyitftvfi cigiffalga ltmwharlit hgetsieahi nkterirlgk
      361 egiiyknpyd fgprknwrlf lgltdgrtwt hlllpsthlp egngltwtti ysekw
//
LOCUS       XP_077501929             364 aa            linear   INV 25-NOV-2025
DEFINITION  palmitoyltransferase ZDHHC16 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077501929
VERSION     XP_077501929.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645803.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..364
                     /product="palmitoyltransferase ZDHHC16 isoform X2"
                     /calculated_mol_wt=42117
     Region          142..301
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; pfam01529"
                     /db_xref="CDD:396215"
     CDS             1..364
                     /gene="LOC144112957"
                     /coded_by="XM_077645803.1:288..1382"
                     /db_xref="GeneID:144112957"
ORIGIN      
        1 mdivmrfiry irrgferlky rvrhkflypr ivfnslvyne fadiyyvgdt cmeplfwivd
       61 hftkamgpif vatvtivact viaiayligi pywwennkyv laialvighw lllnivfhyw
      121 malttnpgtp peasmvpevv sickkciapk pprahhcsvc nrcilkmdhh cpwlnncigh
      181 fthryffmfc syvllgiiyl mifgykiaye eyfgtllaps vglansmiql gnldmsanvt
      241 lhdipgnnrt daakqdfvrk yyitftvfic igiffalgal tmwharlith getsieahin
      301 kterirlgke giiyknpydf gprknwrlfl gltdgrtwth lllpsthlpe gngltwttiy
      361 sekw
//
LOCUS       XP_077501930             664 aa            linear   INV 25-NOV-2025
DEFINITION  signal recognition particle 72 [Amblyomma americanum].
ACCESSION   XP_077501930
VERSION     XP_077501930.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645804.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..664
                     /product="signal recognition particle 72"
                     /calculated_mol_wt=74213
     Region          8..263
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Region          43..70
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          48..151
                     /region_name="SRP_TPR_like"
                     /note="Putative TPR-like repeat; pfam17004"
                     /db_xref="CDD:435703"
     Region          75..99
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          286..>490
                     /region_name="Spy"
                     /note="Predicted O-linked N-acetylglucosamine transferase,
                     SPINDLY family [Posttranslational modification, protein
                     turnover, chaperones]; COG3914"
                     /db_xref="CDD:443119"
     Region          533..583
                     /region_name="SRP72"
                     /note="SRP72 RNA-binding domain; pfam08492"
                     /db_xref="CDD:462494"
     CDS             1..664
                     /gene="Srp72"
                     /coded_by="XM_077645804.1:122..2116"
                     /db_xref="GeneID:144112958"
ORIGIN      
        1 maqtntsaal sgfylelsry gksgeydral kvcdkilhef pteekalqck vvsliqigdf
       61 kdaidvinkn skhigdmafe kayclyrlnd tkeawkllns istqsfrvke lkaqvlyrle
      121 nykecfdvyr dliknsqddy eeeretnlaa viasltmqtr qevkgapdlg egsyelcynk
      181 actllglgky adahrklvqa eelckkylee ddipedeiee emaivrtqla yakqmlgfle
      241 qamklynltl kqrpsdiaii avaannivti nkdqnmfdsk kkikmavaeg leskltsqqr
      301 raialnhcll lyhtgqldqc vkaigmlekn ftnardeavl lraatlhcke kqldkavqll
      361 kdyaehhpsr slsisltlaq lllsqghlnd ccellkslgs ttfklgivsa lvtlyknled
      421 keataavfad aiawyrknkp rsselnlltr esakfflang evqeaanvle qlrkvdphdp
      481 kvlaqlisay svfdpkkakk vsadlppaee itrqvdvdal etmswtmgar yvkkavksea
      541 spitrgqdiv kkkkkkkkgk lpknydpnvd pdperwlprr erstfkkrkd rrgagsaigk
      601 gtqgavgsas egdmtqrttp lpspatsaaa aqsspggqqa sqqgprqqrp lpaqkkkkkk
      661 ggrw
//
LOCUS       XP_077501931             565 aa            linear   INV 25-NOV-2025
DEFINITION  protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase
            2-like [Amblyomma americanum].
ACCESSION   XP_077501931
VERSION     XP_077501931.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645805.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..565
                     /product="protein O-linked-mannose
                     beta-1,4-N-acetylglucosaminyltransferase 2-like"
                     /calculated_mol_wt=64522
     Region          <268..351
                     /region_name="Glyco_transf_61"
                     /note="Glycosyltransferase 61; pfam04577"
                     /db_xref="CDD:428020"
     CDS             1..565
                     /gene="LOC144112959"
                     /coded_by="XM_077645805.1:135..1832"
                     /db_xref="GeneID:144112959"
ORIGIN      
        1 mqlrpshvic lliclladrl ldsayqyadv pctetgsrry trawdsasss vwcrglnyst
       61 rtcvfrnlyy rgdtgdfvfl hgpgttllgd vnerndpalt dlssvkdhga fyftfvdlpv
      121 salsqfrvvd vhtdtlvisr fnpdnlmhvf hddlipvfst lremrscsss eevlncldnv
      181 tlfladerek gpywqlykil vrnqkilhrr qisgwyrfrr aivglrkdst wyqygfampq
      241 gplhanleta gaevkyfvpf flhafgvpat sqinekyali isrtrnrlit nidelvlmve
      301 lhtqltplvi dletkslrev villrdtkfl ismhgsalvl saflqpgsav lemfpyginp
      361 nnytpyktla nlpgmmiaya awvntnknnt vfhpeyepqf ggiyhlsqaa qqqllqseqv
      421 plhlccdnpk wlfhiyqdta vdtsivpllv nlstravdtn eayqrtffpg pvielkceil
      481 qhsgnnrqlt vswlepwnia figfqhchye vwlkssaeve mfhtnltvfd ryiaesvvhv
      541 wvkavcdeqs gsfniypasc ehttl
//
LOCUS       XP_077501932             616 aa            linear   INV 25-NOV-2025
DEFINITION  pre-mRNA-splicing factor Slu7 [Amblyomma americanum].
ACCESSION   XP_077501932
VERSION     XP_077501932.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645806.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..616
                     /product="pre-mRNA-splicing factor Slu7"
                     /calculated_mol_wt=70359
     Region          154..413
                     /region_name="Slu7"
                     /note="Pre-mRNA splicing Prp18-interacting factor;
                     pfam11708"
                     /db_xref="CDD:463326"
     CDS             1..616
                     /gene="Slu7"
                     /coded_by="XM_077645806.1:130..1980"
                     /db_xref="GeneID:144112960"
ORIGIN      
        1 mnqtnlpvse iikskevdep kkqsredwrk akeleearka gtapaavdee gkdinphipq
       61 yissapwyfg akgptlkhqr pqpekqkqfn hisdyysrge fdgkatkyrk gacencgalt
      121 hkrkdclerp rkvgakftnd diapdeknlp slnldydgkr drwngfdpac yqavieeyrk
      181 veeakrqlke dklkhdimnl egistrered sdddvdgded kyadnadmpg tkvdskqrit
      241 vrnlriredv akylrnldpn sayydpktrs mrdnpyknsn kgsdelsfag dnfvrysgdt
      301 qkiaeaqlfa wqayekgvdv hlqaeptkae llnkeflske eefrhsmkds ilekyggaeh
      361 lqmppkelvf aqteeyveys rhgevirgae kpvirskyee hvlinnhtsv wgsywkdfqw
      421 gykcchsfik nsyctgasgk evqavsggam lmpaklmgap laddaahaes nakedtleve
      481 slqpalkeqq desppvvagt vspplaatvf kgtvspekak kkkkkkykka rkekpvkces
      541 ssssdsedsd akaererkek ikeameqidk dqtqetderk rkynsmydvk apteeemeay
      601 ymkrkrdddp mamfip
//
LOCUS       XP_077501933             581 aa            linear   INV 25-NOV-2025
DEFINITION  NADPH-dependent diflavin oxidoreductase 1-like [Amblyomma
            americanum].
ACCESSION   XP_077501933
VERSION     XP_077501933.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645807.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..581
                     /product="NADPH-dependent diflavin oxidoreductase 1-like"
                     /calculated_mol_wt=65974
     Region          3..576
                     /region_name="CysJ"
                     /note="Flavoprotein (flavin reductase) subunit CysJ of
                     sulfite and N-hydroxylaminopurine reductases [Nucleotide
                     transport and metabolism, Inorganic ion transport and
                     metabolism]; COG0369"
                     /db_xref="CDD:440138"
     CDS             1..581
                     /gene="LOC144112961"
                     /coded_by="XM_077645807.1:206..1951"
                     /db_xref="GeneID:144112961"
ORIGIN      
        1 mlgrrflvly gsqtgtaqdv aerigrqarr yrfsvsvlam ddypvtelik eklvvfvcat
       61 tgqgeepdnm kcfwsfllrk slplnslqam rhcviglgds sylkfnftak rlyrrlgqlg
      121 aqclldplya deqhelgvdg mldpwlvefw kivmdlyplp ngclpvseei lpapkyainc
      181 dtasvvtprl sneklfnatv vcnqrvtspd hfqdvrlikl niegsgmtfe pgdvvvvype
      241 nceedideff rlfslkadmh itlapmeegt pfphflaapf sldicvrkyf dltyipkrsf
      301 felfwhfggs dlekerlref sttggqedlv eyairprrtv levftdfpht tanvplaylf
      361 dlippirpry fsianslryh pgqihilaai vnfrtklkkp rrglctrfla sleptrqpsv
      421 tvttrkgslr mppddvpaim vgpgtgcapf cgiiqdraqr gisrnllffg crnakndfff
      481 ekewtqlgan glldlvtafs rdqdhkiyvq hriaehedkv wqllcnggvl yiagnakdmv
      541 psvrdafkav vkrsgsltde naeellknle ksrrlqieaw s
//
LOCUS       XP_077501934             121 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112964 [Amblyomma americanum].
ACCESSION   XP_077501934
VERSION     XP_077501934.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645808.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..121
                     /product="uncharacterized protein LOC144112964"
                     /calculated_mol_wt=11970
     CDS             1..121
                     /gene="LOC144112964"
                     /coded_by="XM_077645808.1:2183..2548"
                     /db_xref="GeneID:144112964"
ORIGIN      
        1 mdcsqslset salvrragep aavsgepvsk gyvqsrlsqs lllfqedadg asptgstlnq
       61 acshasgfim vqsdhtyagp steasassle pgtagvaatl sasadlspga pfsssplsye
      121 g
//
LOCUS       XP_077501935            2864 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: huntingtin [Amblyomma americanum].
ACCESSION   XP_077501935
VERSION     XP_077501935.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645809.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (GKSP01006189.1) annotated
            using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts  :: corrected 2 indels
            assembly gap :: added 1890 transcript bases to patch genome
                            assembly gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..2864
                     /product="LOW QUALITY PROTEIN: huntingtin"
                     /calculated_mol_wt=316709
     Region          1315..1354
                     /region_name="DUF3652"
                     /note="Huntingtin protein region; pfam12372"
                     /db_xref="CDD:463554"
     CDS             1..2864
                     /gene="htt"
                     /coded_by="XM_077645809.1:67..8661"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 1 base in 1 codon;
                     deleted 1 base in 1 codon; added 1824 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144112965"
ORIGIN      
        1 masiekliks fealkvvqps aggeepllkk ketapskkek iihctniadv icttsvrtys
       61 dfpkllgisv esfffccdde dsdvrmvade clnrtiktll dshlgrlqae lykeikkngp
      121 grslrvalgk fgdlchlikt qkcraytvnl ipcfvrisqr seeesvqeal antvvrampm
      181 lgqftndndi kmllrtflpn lscpsalvrr caalslvtvc qwsrkpnvfl lwllndlfsl
      241 vlpvqenhpa htivgvlvcf rhliphlgsk pakdvpalkg aekagptleh flkiyelllh
      301 hisntdqivv taalealnql lttpppallq vllspsgvaq sfihapnrrp vsrtdtsmmg
      361 sslnideeve lvdesddspl vtcaiqsrts sehqseaedg sagfpediea asiascdged
      421 sdfsiastap thllaldspm rrpcslkkld lkaeeegvwd kvsinsspgf kvefvlgnlg
      481 syrdadvplk ycarllastf lltgtngvlm pdgnvrvsmk vlamgsltrv lllyppglfl
      541 dlhitqvpde dddqkiwdvl efvnhsdpql cgqtalllgh ylghtlklas hgwnywarkq
      601 trrpdlpklv dlvgkitrva gssssvssrt gvqatrlcia gllhschanl vpslleacmk
      661 lkdnaywlvk velvemigel pyralqhiss vstsitksvc pwmptyqeil vhevlfellg
      721 dedvrvrtav taalakcvpq lyypedhpge savsaeaeve sslllgwllh dawtpgppqv
      781 hglvppfsck aprglsvyte aalsriisll wkkivtssnk yllhgccqxl anlvleypvt
      841 vytfawssvl gssssvsspl tlrsqasvss ssvtpespqe gtsesggllv hlvallvasp
      901 ltldihvhqh vlttaghlla gacfrcirpq dieamasqec eepwasmrdr hlvplldklf
      961 qhtmrllnvf ahtleeqasp srgglglsgg palspirrrs rvredaalat tgtatvrgst
     1021 skltseldkt sksqvglghf tslpqymkly evlkaahsny kvsldtagse kflgllkavl
     1081 itmgqileva qlqdvnrhve ellgylrlsm tveplttvqc vrqllkalfg tnltslwegc
     1141 anndslsail sapstglyhn ccsypyacls kllasyssrs plltadattl gswlswmrrg
     1201 cekkisallk psnkrgkaal aahirlfepv vlqalkqytv sssshlqasv ldllaqlvhl
     1261 rvnyclldsd qifigfvikq fefieegqiq naevlipqif yflvllsyer yhskpvismp
     1321 eiiqlcdrlm asgqppdryv ipalqaived lfllpsanrt dsrkeletqr evvvsvllrl
     1381 iqypevlell lavvhhsyqe gedrwkkfsr qlidvilplm srtqvhldtr hslsvlqrlf
     1441 dsvspsvfrp vdillkalfv cpmdpvgaep qtgfraerwm clvlmslrvl laytkeevvl
     1501 srladlmlpl rvphalapea lydpldidsl gdnmekavar yliqvvnvyg khlckillqs
     1561 ppagtdtvfp fqqmssllly lthmfqsgsf crvataamqa vhaessigte vcgieeinqh
     1621 flslaysypt lsvqwcnila llnygeqgfw srvlwpsigs svgtagmarr nlntpsyscn
     1681 felvsqagli llcdylceni ydaepmtwli vnhvseiirl sseppvsdfi svihrnpaas
     1741 glfvqavltr cetkssqpsf vskllrcleg ihpaqsaall allvekilvt phlslartce
     1801 alaqrqleli ltepgfaars ptalqdlagl pefmrstklh lrhshllall ekllkadasq
     1861 hlacgegadl vtppklhvnk ewflaqvrer ccsgqacave csqllanlsh edteaiiqlk
     1921 dvgpsvvcas lrlgaqrsst gesalyraar scvlgqtrrl vaelpqphhp flaaavaecp
     1981 rearhqarli alledaqvwd tlcrlappla tyldglaala admpidsled varfavlcae
     2041 airwmtetpd cfscnhleef lqaldavlss pslssliglq khvtwvcsit taiyhlvsrl
     2101 kgwksfpvkv tprkpkdssp eymhvqqacv hvaelvgwle rsgkeahipv fikttlhrvi
     2161 kgvarlplvn sfvrtppimw qlnwvphvsg ewhtlvpppp adflldrdll qeyiyrvnll
     2221 gwisrqqfee twmallgvln tspsaeqeta edeqervavq clavrcitsl llqslqlpqp
     2281 gnphsstllh qprdkpfafv htrcgkrlqa vrtpvheall kmlklnssms wgrsniekmc
     2341 rhteyslgqv sveymcvavg laegadltst ssplssssss sssqysppgg svgclrreas
     2401 laaagldlrs clhflldlyt qwlgppdaig qplltetirs vvmlsdifme ssqfdwmlsl
     2461 fmdmyrhhps edeilaqyli lgiakaaavv sldsdmqeki rkfvdlglrc pmpstqllsl
     2521 hgllymlaqp gdatspllps atehltkhld dssfkdsepn mlalwalaff laenysnkqd
     2581 ltpkivqvsl nlcngdnlpl slylcilhgl erlllagvie sqdneliikv cierirhskp
     2641 teampalgvm lsalyiaqgv nkrrsqpsie lepanseqra aaleratllf drikrgypfe
     2701 adlisqilpg flgeffpaqd ilnkvigefl snqqpypqfl aavlfkvceg lhegnheelm
     2761 qdwvllslsn ftqrsplama iwslsccfva vtatvwlral fpliqgrmgm feehdkqlfy
     2821 isaldfqrql vneqhkmqfy niikgvaspd tpyaellkql pqpp
//
LOCUS       XP_077501937             380 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112966 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501937
VERSION     XP_077501937.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645811.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..380
                     /product="uncharacterized protein LOC144112966 isoform X1"
                     /calculated_mol_wt=42252
     Region          181..>359
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     CDS             1..380
                     /gene="LOC144112966"
                     /coded_by="XM_077645811.1:284..1426"
                     /db_xref="GeneID:144112966"
ORIGIN      
        1 mgdelqrldd evdqlatrrq gvpvslpmds edmtaspprl lpvsgvlvaa npavvrpvaf
       61 ravhwagget ddgcsssgsv ltlheerlll aeqrpsghhs apllerqsra patrtafhpa
      121 cckvppfhqs mphfhckera psrassdggg samldvldsg hssaallaee eeapspsdsg
      181 vaeleaqlre kdaeiahlrq tlerneqaii qvyeekeqlw rrrledlrdh yeerlrmqrd
      241 vpagqdgspa apagaveaas vrahqvswld tpqldsevae lttlmedssl agndnggdev
      301 rllrlelqhc rrqlqlalqg ieeerqhwqh erdqaiqfrr hleasywqvf nqnrellrav
      361 shaslpheql dgwyhsessc
//
LOCUS       XP_077501938             380 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112966 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501938
VERSION     XP_077501938.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645812.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..380
                     /product="uncharacterized protein LOC144112966 isoform X1"
                     /calculated_mol_wt=42252
     Region          181..>359
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     CDS             1..380
                     /gene="LOC144112966"
                     /coded_by="XM_077645812.1:391..1533"
                     /db_xref="GeneID:144112966"
ORIGIN      
        1 mgdelqrldd evdqlatrrq gvpvslpmds edmtaspprl lpvsgvlvaa npavvrpvaf
       61 ravhwagget ddgcsssgsv ltlheerlll aeqrpsghhs apllerqsra patrtafhpa
      121 cckvppfhqs mphfhckera psrassdggg samldvldsg hssaallaee eeapspsdsg
      181 vaeleaqlre kdaeiahlrq tlerneqaii qvyeekeqlw rrrledlrdh yeerlrmqrd
      241 vpagqdgspa apagaveaas vrahqvswld tpqldsevae lttlmedssl agndnggdev
      301 rllrlelqhc rrqlqlalqg ieeerqhwqh erdqaiqfrr hleasywqvf nqnrellrav
      361 shaslpheql dgwyhsessc
//
LOCUS       XP_077501939             371 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112966 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501939
VERSION     XP_077501939.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645813.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..371
                     /product="uncharacterized protein LOC144112966 isoform X2"
                     /calculated_mol_wt=41053
     Region          172..>350
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     CDS             1..371
                     /gene="LOC144112966"
                     /coded_by="XM_077645813.1:172..1287"
                     /db_xref="GeneID:144112966"
ORIGIN      
        1 mgawtslpte pgvpvslpmd sedmtasppr llpvsgvlva anpavvrpva fravhwagge
       61 tddgcsssgs vltlheerll laeqrpsghh sapllerqsr apatrtafhp acckvppfhq
      121 smphfhcker apsrassdgg gsamldvlds ghssaallae eeeapspsds gvaeleaqlr
      181 ekdaeiahlr qtlerneqai iqvyeekeql wrrrledlrd hyeerlrmqr dvpagqdgsp
      241 aapagaveaa svrahqvswl dtpqldseva elttlmedss lagndnggde vrllrlelqh
      301 crrqlqlalq gieeerqhwq herdqaiqfr rhleasywqv fnqnrellra vshaslpheq
      361 ldgwyhsess c
//
LOCUS       XP_077501940             353 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112966 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501940
VERSION     XP_077501940.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645814.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..353
                     /product="uncharacterized protein LOC144112966 isoform X3"
                     /calculated_mol_wt=39232
     Region          154..>332
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     CDS             1..353
                     /gene="LOC144112966"
                     /coded_by="XM_077645814.1:260..1321"
                     /db_xref="GeneID:144112966"
ORIGIN      
        1 mdsedmtasp prllpvsgvl vaanpavvrp vafravhwag getddgcsss gsvltlheer
       61 lllaeqrpsg hhsapllerq srapatrtaf hpacckvppf hqsmphfhck erapsrassd
      121 gggsamldvl dsghssaall aeeeeapsps dsgvaeleaq lrekdaeiah lrqtlerneq
      181 aiiqvyeeke qlwrrrledl rdhyeerlrm qrdvpagqdg spaapagave aasvrahqvs
      241 wldtpqldse vaelttlmed sslagndngg devrllrlel qhcrrqlqla lqgieeerqh
      301 wqherdqaiq frrhleasyw qvfnqnrell ravshaslph eqldgwyhse ssc
//
LOCUS       XP_077501941             339 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112966 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077501941
VERSION     XP_077501941.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645815.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..339
                     /product="uncharacterized protein LOC144112966 isoform X4"
                     /calculated_mol_wt=36379
     Region          183..>236
                     /region_name="Atg16_CCD"
                     /note="Coiled-coiled domain of autophagy-related 16
                     (Atg16) family proteins; cl46300"
                     /db_xref="CDD:480640"
     CDS             1..339
                     /gene="LOC144112966"
                     /coded_by="XM_077645815.1:280..1299"
                     /db_xref="GeneID:144112966"
ORIGIN      
        1 mgdelqrldd evdqlatrrq gvpvslpmds edmtaspprl lpvsgvlvaa npavvrpvaf
       61 ravhwagget ddgcsssgsv ltlheerlll aeqrpsghhs apllerqsra patrtafhpa
      121 cckvppfhqs mphfhckera psrassdggg samldvldsg hssaallaee eeapspsdsg
      181 vaeleaqlre kdaeiahlrq tlerneqaii qvyeekeqlw rrrledlrdh yeerlrmqrd
      241 vpagqdgspa apagaveaas vrahqvswld tpqldsevae lttlmedssl agyrggasal
      301 atrarpshpv paasggella svqpeprvaa crvarqlas
//
LOCUS       XP_077501942             362 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase TM129 [Amblyomma americanum].
ACCESSION   XP_077501942
VERSION     XP_077501942.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645816.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (GKSP01012420.1) annotated
            using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 43 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..362
                     /product="E3 ubiquitin-protein ligase TM129"
                     /calculated_mol_wt=40831
     Region          19..358
                     /region_name="Tmpp129"
                     /note="Putative transmembrane protein precursor;
                     pfam10272"
                     /db_xref="CDD:463039"
     CDS             1..362
                     /gene="LOC144112967"
                     /coded_by="XM_077645816.1:53..1141"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 43 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144112967"
ORIGIN      
        1 mateavwfsv vyamicvyil tpptefvtag ltlthlvgrf idkedvkfve hhiqrtlltv
       61 ivhtflplgy fvgftnlied dsvvelwqsn llwqgglais clvftagivl aftwsmadwa
      121 rhpvtqrllq ygspwrliam rinvefsqqv dkfisgagsl trtvvtnswi ikatpynlhl
      181 iqqtdavlel vgsaehsvsh vsgmgiqyld ilvrsrrpgi psftirvnsm eyndlkeklq
      241 apldnvrniv ihrslsdrft dafrshveqn akyrlpagme lepcigcmqt tanvklqklc
      301 dealvgqcqg clcrpmwcld cmgrwfasrq dqarpetwlg srcfcptcrs vfcmldvciv
      361 ea
//
LOCUS       XP_077501943             241 aa            linear   INV 25-NOV-2025
DEFINITION  tetratricopeptide repeat protein 1-like [Amblyomma americanum].
ACCESSION   XP_077501943
VERSION     XP_077501943.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645817.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..241
                     /product="tetratricopeptide repeat protein 1-like"
                     /calculated_mol_wt=27198
     Region          <69..>176
                     /region_name="NlpI"
                     /note="Lipoprotein NlpI, contains TPR repeats [Cell
                     wall/membrane/envelope biogenesis]; COG4785"
                     /db_xref="CDD:443815"
     Region          71..99
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(72,75..76,79..80,82,106,114..115,118..119,121..122,
                     145,148..149,152..153,156)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          104..139
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          144..172
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     CDS             1..241
                     /gene="LOC144112968"
                     /coded_by="XM_077645817.1:159..884"
                     /db_xref="GeneID:144112968"
ORIGIN      
        1 masspksegm avtedstevs qeklqstdhp tdsenvegde eedlyiidee eleklektmn
       61 deerksrker alklkaegnv sfktgqyada mesytealri cplsssaers ilysnrgatw
      121 arlekkkvai kdctkaieln psylkpilkr aqlyketnnl ddalkdyqrv leldpsigea
      181 rhacmtlphe ikerneklqa emlgklkelg nlvlkpfgms tdnfklvkeg egggykiqfq
      241 k
//
LOCUS       XP_077501944            2282 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112969 [Amblyomma americanum].
ACCESSION   XP_077501944
VERSION     XP_077501944.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645818.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2282
                     /product="uncharacterized protein LOC144112969"
                     /calculated_mol_wt=249189
     Region          <1118..>1210
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     CDS             1..2282
                     /gene="LOC144112969"
                     /coded_by="XM_077645818.1:68..6916"
                     /db_xref="GeneID:144112969"
ORIGIN      
        1 mhreaalvlv lfalkisqla sglrgpggce slvralgpda rslrpylqrh lavpgvamre
       61 dlfeclleel prwpaaqlga dlgriaqlae rappadgqaq aqmlrllwat paqrlpgevv
      121 rraraagral lgratlpepv vrryealfkd qsaqarvvar qlaehafdcr rappgdseld
      181 rlpapsarha lvilshfara rscagstllp sgpsdeaaai mwqapslfdh gypkslgmqe
      241 seaadysrar tiekeraynd mlrfvresmr naslsslsie lllkigidva tncrppgstv
      301 allrsiitel qrrggispen nrvfthvlss fqrqknctkv sirdlinahd ylvpqlledf
      361 qksvlfesps vraylvtels iyakmarqip aerrvallgv atalltiswp pgkvldllid
      421 sasllqfgsv srqmarkmip allrihtrpy yewlspkrks ylyltllrli eqldkmdeni
      481 lvaietvvas pceklnlvsv lfrmletptv sskafsyiec lilyllkvpr wfkidwsvii
      541 vpptpkgpch pqtwhtttgt pglsvqttpt aamtetpsts ieetttpegq vtskkttsys
      601 tttpgsatte vptttetgae tptspsfapt tsketttgtp ssevstyett stttgrtest
      661 mtfsstvtpt gtetsttasn tipttevfst tssatetita tpsgetttst vmssempstt
      721 tmittttera lsqtpstett gfttttetpg vstsietsta tkstlittst ertatptels
      781 setptmtpst tsgsttstet paasestevt tstaftstkt ttetpaases kemttstaft
      841 stmtttetps asqstlttsk esttttsyet tstttttetp tatpsfgttt stettfittt
      901 etptgtpsrw sttpsavttt etvpevtpsi vttpaessts ftetpsgths tatttstgyt
      961 ttstttsapt etpsesytst ttststatps etystattts tstetpsqmy stgttsttet
     1021 sseshltstt tststetpsg systetttst stqtpsegys tftttststq tssegysttt
     1081 tttstgsset pigststygt ttetpagths tetttstafi kttstetpsa evstqgttst
     1141 etptggysvs ttttpgqvtl tptqvvyttp gtstsvtypq qptvpeqpen rendtttptg
     1201 fgstivttdn llpmgttvvt lptptgtlkv ftvpsfpesa tvkfniekis hhithtnest
     1261 tnilaiiesi tnmshptdit mmiegahnit ighpdierav dekliqiive altaeqlqgi
     1321 vtdrterlvm elyriiktgv rirldilkti ltlkmtatlr eniisilvnk tyrgpteevh
     1381 kvleillpyl avhhvstltt kefihiliqy vqttnrssis gdlrhfliei ieeltrspki
     1441 pdtskqrlta ilqillkevp vqrtnfvevr kylqnpkapi sltlllsiqp nprgtapgss
     1501 etirilasvv kqsagnpvvv gnvvslllrl lstnnvprmq igplltslnk ivhrrdliap
     1561 hlriaaltdc lilygndgfq ylsptarstv lttlvsllng rvddylvtrl lsfkwpnlkt
     1621 rrtfntlrrd visvkpngpi egltavnqil lvigliapnn gsspedlhni avklnshppe
     1681 qpkyprsiry iiasllkkkp vqltdipksg itplsyvhwp tyemppdlvd niakevedrd
     1741 ykithltkel sfdlvspald ptvrgnllve smrllttdqm tvtlaqqllr aihaavtlvp
     1801 slwpknslll lqdiptvitr piirslpplq tailpsllhh minraqhipk pildtlikfa
     1861 psqeyatevv tdiiaallkh rnvpeqadls mklvkmlder tgssslplkd vadlltrlkd
     1921 rlsrtardsp elkpvikyin evlrrlqsep lsalrnlkky lrtlqgpapv elvrsifedf
     1981 pswtpndlqt vvslvrdama krrepevvgs vaivamrllr lynytdgtek alvkvidqar
     2041 dsvipqplkl vlvndilfvl atkvthkses hvnvqtllrl ldavlrsgdl pttstasves
     2101 hfikavaafv dnkfalrkvt relrvrladl lrrldlqhfi pmeilgkidk qlrkemwtvi
     2161 rkvhttteer sitqflqylk tahrdeivrm dessmrnlpk vmlplppral snviagvedv
     2221 lrlnheiihr dlarqlltiv qtaisspmfp atllprsksv mdalkrdirh enargglypp
     2281 am
//
LOCUS       XP_077501945             304 aa            linear   INV 25-NOV-2025
DEFINITION  ribonuclease H2 subunit A [Amblyomma americanum].
ACCESSION   XP_077501945
VERSION     XP_077501945.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645819.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..304
                     /product="ribonuclease H2 subunit A"
                     /calculated_mol_wt=33429
     Region          31..251
                     /region_name="RNase_HII_eukaryota_like"
                     /note="Eukaryotic RNase HII; cd07181"
                     /db_xref="CDD:260002"
     Site            order(34..39,69,113..115,142..144,168,170,184,214,
                     239..241)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260002"
     Site            order(100,103..104,226..229,231,237,246,250..251)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:260002"
     CDS             1..304
                     /gene="LOC144112971"
                     /coded_by="XM_077645819.1:85..999"
                     /db_xref="GeneID:144112971"
ORIGIN      
        1 mdldsfnknn kqnalvksiv pditkaepci lgvdeagrgp vlgpmvygia ycplsakdql
       61 kelgfadskt lteekreell svieknssfl gfmvelispv viselmlhvs khnlnsishd
      121 sainlirral eegvnvaevy vdtvgppeky qkklealfpg ikvtvakkad atypivsaas
      181 icakvardra vrswqfpegi nvkpgdygsg ypndpltkkf ltdnmdvvfg fpgivrfsws
      241 taeklledha ativwdeedq ngaaklgmas iqsffkhdpk rgkkptekes sfftrrgmsq
      301 vhql
//
LOCUS       XP_077501946             976 aa            linear   INV 25-NOV-2025
DEFINITION  MMS19 nucleotide excision repair protein isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501946
VERSION     XP_077501946.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645820.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..976
                     /product="MMS19 nucleotide excision repair protein isoform
                     X1"
                     /calculated_mol_wt=107865
     Region          47..301
                     /region_name="MMS19_N"
                     /note="Dos2-interacting transcription regulator of
                     RNA-Pol-II; pfam14500"
                     /db_xref="CDD:433995"
     Region          <653..928
                     /region_name="MMS19_C"
                     /note="RNAPII transcription regulator C-terminal;
                     pfam12460"
                     /db_xref="CDD:463594"
     Region          861..894
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          899..926
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          938..963
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..976
                     /gene="Mms19"
                     /coded_by="XM_077645820.1:32..2962"
                     /db_xref="GeneID:144112972"
ORIGIN      
        1 maatvlyedd akfqekvasy vnvikgdgde llrtvenmel lsrrgilthe npeervagvk
       61 fitliiqglp qrclsnsqat alvryyvnkl edqpsmvpfv irglyelvvs qgpvdddeid
      121 qllqailcev qmpgllqaer yvvyrmlaym llnytsglhk mgskfvhaci qalegerdpr
      181 cllqvfaiip mlcnefilde leesvfdvca cyfpvdfnpp gedvgritre elaealltcm
      241 tgsagfgkyc vplaveklds dltvakldsl kvlirgaevf ppvcfvdkmi liwkqihnvv
      301 fvnrhealip talscltsis kaisgydkna ltalfrvvwk dlqrgeapgm gkvleafasa
      361 saeacsvalv ealpsilqll akdeglrpml vlesttnvls fyanlckhee atqelnrllg
      421 qladllctlv tgndhelalr aqaavvatlp vkgfltgsql qilkevllqv atsvdapavg
      481 dehilllaea artnclghdl whilkqeiak gycpgkseri lsmatacpgs gismaivlpc
      541 vvasfvcatk dnqtvywnll akclvgitcq aekygmklch vsllrkvvfa wmdamkrghe
      601 nesietfhev svlvqklsei ssgrdmrdii shveefcvdt slatssllll kcivchvrpe
      661 llsanqrlve lltgahcrcp tqlvaqclag yvnklpdadl ektlgciqps lepdwtllkt
      721 elllwvikgl llrgypnlap ytkllkellk deklgrhaak gfqqilqplv ltmdghcivk
      781 lmhpqrffve tvdfliegfn svtaegknqi sslvlfqapw lnteicsfll wltavkdnfl
      841 malccqlayv pkpvvntyid kilpilvtal ssaeesvvae cvlpcltenv glmashldsv
      901 leqllrlakk pfpmdvrrsa lqcllrlsti kenvmlykqe vlqgltpcla drkrivrqva
      961 aqasslwhmv gepsgv
//
LOCUS       XP_077501947             971 aa            linear   INV 25-NOV-2025
DEFINITION  MMS19 nucleotide excision repair protein isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501947
VERSION     XP_077501947.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645821.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..971
                     /product="MMS19 nucleotide excision repair protein isoform
                     X2"
                     /calculated_mol_wt=107239
     Region          38..296
                     /region_name="MMS19_N"
                     /note="Dos2-interacting transcription regulator of
                     RNA-Pol-II; pfam14500"
                     /db_xref="CDD:433995"
     Region          <648..923
                     /region_name="MMS19_C"
                     /note="RNAPII transcription regulator C-terminal;
                     pfam12460"
                     /db_xref="CDD:463594"
     Region          856..889
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          894..921
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          933..958
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..971
                     /gene="Mms19"
                     /coded_by="XM_077645821.1:32..2947"
                     /db_xref="GeneID:144112972"
ORIGIN      
        1 maatvlyedd akfqekvasy vnvikgdgde llrtvenmeg ilthenpeer vagvkfitli
       61 iqglpqrcls nsqatalvry yvnkledqps mvpfvirgly elvvsqgpvd ddeidqllqa
      121 ilcevqmpgl lqaeryvvyr mlaymllnyt sglhkmgskf vhaciqaleg erdprcllqv
      181 faiipmlcne fildeleesv fdvcacyfpv dfnppgedvg ritreelaea lltcmtgsag
      241 fgkycvplav ekldsdltva kldslkvlir gaevfppvcf vdkmiliwkq ihnvvfvnrh
      301 ealiptalsc ltsiskaisg ydknaltalf rvvwkdlqrg eapgmgkvle afasasaeac
      361 svalvealps ilqllakdeg lrpmlvlest tnvlsfyanl ckheeatqel nrllgqladl
      421 lctlvtgndh elalraqaav vatlpvkgfl tgsqlqilke vllqvatsvd apavgdehil
      481 llaeaartnc lghdlwhilk qeiakgycpg kserilsmat acpgsgisma ivlpcvvasf
      541 vcatkdnqtv ywnllakclv gitcqaekyg mklchvsllr kvvfawmdam krghenesie
      601 tfhevsvlvq klseissgrd mrdiishvee fcvdtslats sllllkcivc hvrpellsan
      661 qrlvelltga hcrcptqlva qclagyvnkl pdadlektlg ciqpslepdw tllktelllw
      721 vikglllrgy pnlapytkll kellkdeklg rhaakgfqqi lqplvltmdg hcivklmhpq
      781 rffvetvdfl iegfnsvtae gknqisslvl fqapwlntei csfllwltav kdnflmalcc
      841 qlayvpkpvv ntyidkilpi lvtalssaee svvaecvlpc ltenvglmas hldsvleqll
      901 rlakkpfpmd vrrsalqcll rlstikenvm lykqevlqgl tpcladrkri vrqvaaqass
      961 lwhmvgepsg v
//
LOCUS       XP_077501948             948 aa            linear   INV 25-NOV-2025
DEFINITION  MMS19 nucleotide excision repair protein isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501948
VERSION     XP_077501948.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645822.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..948
                     /product="MMS19 nucleotide excision repair protein isoform
                     X3"
                     /calculated_mol_wt=104660
     Region          47..301
                     /region_name="MMS19_N"
                     /note="Dos2-interacting transcription regulator of
                     RNA-Pol-II; pfam14500"
                     /db_xref="CDD:433995"
     Region          <653..900
                     /region_name="MMS19_C"
                     /note="RNAPII transcription regulator C-terminal;
                     pfam12460"
                     /db_xref="CDD:463594"
     Region          833..866
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          871..898
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          910..935
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..948
                     /gene="Mms19"
                     /coded_by="XM_077645822.1:32..2878"
                     /db_xref="GeneID:144112972"
ORIGIN      
        1 maatvlyedd akfqekvasy vnvikgdgde llrtvenmel lsrrgilthe npeervagvk
       61 fitliiqglp qrclsnsqat alvryyvnkl edqpsmvpfv irglyelvvs qgpvdddeid
      121 qllqailcev qmpgllqaer yvvyrmlaym llnytsglhk mgskfvhaci qalegerdpr
      181 cllqvfaiip mlcnefilde leesvfdvca cyfpvdfnpp gedvgritre elaealltcm
      241 tgsagfgkyc vplaveklds dltvakldsl kvlirgaevf ppvcfvdkmi liwkqihnvv
      301 fvnrhealip talscltsis kaisgydkna ltalfrvvwk dlqrgeapgm gkvleafasa
      361 saeacsvalv ealpsilqll akdeglrpml vlesttnvls fyanlckhee atqelnrllg
      421 qladllctlv tgndhelalr aqaavvatlp vkgfltgsql qilkevllqv atsvdapavg
      481 dehilllaea artnclghdl whilkqeiak gycpgkseri lsmatacpgs gismaivlpc
      541 vvasfvcatk dnqtvywnll akclvgitcq aekygmklch vsllrkvvfa wmdamkrghe
      601 nesietfhev svlvqklsei ssgrdmrdii shveefcvdt slatssllll kcivchvrpe
      661 llsanqrlve lltgahcrcp tqlvaqclag yvnklpdadl ektlgciqps lepdwtllkt
      721 elllwvikgl llrgypnlap ytkllkellk deklgrhaak gfqqilqplv ltmdghcivk
      781 lmhpqrffve tvdfliegfn svtaeavkdn flmalccqla yvpkpvvnty idkilpilvt
      841 alssaeesvv aecvlpclte nvglmashld svleqllrla kkpfpmdvrr salqcllrls
      901 tikenvmlyk qevlqgltpc ladrkrivrq vaaqasslwh mvgepsgv
//
LOCUS       XP_077501950             943 aa            linear   INV 25-NOV-2025
DEFINITION  MMS19 nucleotide excision repair protein isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077501950
VERSION     XP_077501950.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645824.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..943
                     /product="MMS19 nucleotide excision repair protein isoform
                     X4"
                     /calculated_mol_wt=104035
     Region          38..296
                     /region_name="MMS19_N"
                     /note="Dos2-interacting transcription regulator of
                     RNA-Pol-II; pfam14500"
                     /db_xref="CDD:433995"
     Region          <648..895
                     /region_name="MMS19_C"
                     /note="RNAPII transcription regulator C-terminal;
                     pfam12460"
                     /db_xref="CDD:463594"
     Region          828..861
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          866..893
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          905..930
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..943
                     /gene="Mms19"
                     /coded_by="XM_077645824.1:32..2863"
                     /db_xref="GeneID:144112972"
ORIGIN      
        1 maatvlyedd akfqekvasy vnvikgdgde llrtvenmeg ilthenpeer vagvkfitli
       61 iqglpqrcls nsqatalvry yvnkledqps mvpfvirgly elvvsqgpvd ddeidqllqa
      121 ilcevqmpgl lqaeryvvyr mlaymllnyt sglhkmgskf vhaciqaleg erdprcllqv
      181 faiipmlcne fildeleesv fdvcacyfpv dfnppgedvg ritreelaea lltcmtgsag
      241 fgkycvplav ekldsdltva kldslkvlir gaevfppvcf vdkmiliwkq ihnvvfvnrh
      301 ealiptalsc ltsiskaisg ydknaltalf rvvwkdlqrg eapgmgkvle afasasaeac
      361 svalvealps ilqllakdeg lrpmlvlest tnvlsfyanl ckheeatqel nrllgqladl
      421 lctlvtgndh elalraqaav vatlpvkgfl tgsqlqilke vllqvatsvd apavgdehil
      481 llaeaartnc lghdlwhilk qeiakgycpg kserilsmat acpgsgisma ivlpcvvasf
      541 vcatkdnqtv ywnllakclv gitcqaekyg mklchvsllr kvvfawmdam krghenesie
      601 tfhevsvlvq klseissgrd mrdiishvee fcvdtslats sllllkcivc hvrpellsan
      661 qrlvelltga hcrcptqlva qclagyvnkl pdadlektlg ciqpslepdw tllktelllw
      721 vikglllrgy pnlapytkll kellkdeklg rhaakgfqqi lqplvltmdg hcivklmhpq
      781 rffvetvdfl iegfnsvtae avkdnflmal ccqlayvpkp vvntyidkil pilvtalssa
      841 eesvvaecvl pcltenvglm ashldsvleq llrlakkpfp mdvrrsalqc llrlstiken
      901 vmlykqevlq gltpcladrk rivrqvaaqa sslwhmvgep sgv
//
LOCUS       XP_077501951             845 aa            linear   INV 25-NOV-2025
DEFINITION  MMS19 nucleotide excision repair protein isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077501951
VERSION     XP_077501951.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645825.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..845
                     /product="MMS19 nucleotide excision repair protein isoform
                     X5"
                     /calculated_mol_wt=93143
     Region          <1..170
                     /region_name="MMS19_N"
                     /note="Dos2-interacting transcription regulator of
                     RNA-Pol-II; pfam14500"
                     /db_xref="CDD:433995"
     Region          <522..797
                     /region_name="MMS19_C"
                     /note="RNAPII transcription regulator C-terminal;
                     pfam12460"
                     /db_xref="CDD:463594"
     Region          730..763
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          768..795
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          807..832
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..845
                     /gene="Mms19"
                     /coded_by="XM_077645825.1:647..3184"
                     /db_xref="GeneID:144112972"
ORIGIN      
        1 mpgllqaery vvyrmlayml lnytsglhkm gskfvhaciq alegerdprc llqvfaiipm
       61 lcnefildel eesvfdvcac yfpvdfnppg edvgritree laealltcmt gsagfgkycv
      121 plavekldsd ltvakldslk vlirgaevfp pvcfvdkmil iwkqihnvvf vnrhealipt
      181 alscltsisk aisgydknal talfrvvwkd lqrgeapgmg kvleafasas aeacsvalve
      241 alpsilqlla kdeglrpmlv lesttnvlsf yanlckheea tqelnrllgq ladllctlvt
      301 gndhelalra qaavvatlpv kgfltgsqlq ilkevllqva tsvdapavgd ehilllaeaa
      361 rtnclghdlw hilkqeiakg ycpgkseril smatacpgsg ismaivlpcv vasfvcatkd
      421 nqtvywnlla kclvgitcqa ekygmklchv sllrkvvfaw mdamkrghen esietfhevs
      481 vlvqklseis sgrdmrdiis hveefcvdts latssllllk civchvrpel lsanqrlvel
      541 ltgahcrcpt qlvaqclagy vnklpdadle ktlgciqpsl epdwtllkte lllwvikgll
      601 lrgypnlapy tkllkellkd eklgrhaakg fqqilqplvl tmdghcivkl mhpqrffvet
      661 vdfliegfns vtaegknqis slvlfqapwl nteicsfllw ltavkdnflm alccqlayvp
      721 kpvvntyidk ilpilvtals saeesvvaec vlpcltenvg lmashldsvl eqllrlakkp
      781 fpmdvrrsal qcllrlstik envmlykqev lqgltpclad rkrivrqvaa qasslwhmvg
      841 epsgv
//
LOCUS       XP_077501952             817 aa            linear   INV 25-NOV-2025
DEFINITION  MMS19 nucleotide excision repair protein isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077501952
VERSION     XP_077501952.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645826.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..817
                     /product="MMS19 nucleotide excision repair protein isoform
                     X6"
                     /calculated_mol_wt=89938
     Region          <1..170
                     /region_name="MMS19_N"
                     /note="Dos2-interacting transcription regulator of
                     RNA-Pol-II; pfam14500"
                     /db_xref="CDD:433995"
     Region          <522..769
                     /region_name="MMS19_C"
                     /note="RNAPII transcription regulator C-terminal;
                     pfam12460"
                     /db_xref="CDD:463594"
     Region          702..735
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          740..767
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          779..804
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..817
                     /gene="Mms19"
                     /coded_by="XM_077645826.1:647..3100"
                     /db_xref="GeneID:144112972"
ORIGIN      
        1 mpgllqaery vvyrmlayml lnytsglhkm gskfvhaciq alegerdprc llqvfaiipm
       61 lcnefildel eesvfdvcac yfpvdfnppg edvgritree laealltcmt gsagfgkycv
      121 plavekldsd ltvakldslk vlirgaevfp pvcfvdkmil iwkqihnvvf vnrhealipt
      181 alscltsisk aisgydknal talfrvvwkd lqrgeapgmg kvleafasas aeacsvalve
      241 alpsilqlla kdeglrpmlv lesttnvlsf yanlckheea tqelnrllgq ladllctlvt
      301 gndhelalra qaavvatlpv kgfltgsqlq ilkevllqva tsvdapavgd ehilllaeaa
      361 rtnclghdlw hilkqeiakg ycpgkseril smatacpgsg ismaivlpcv vasfvcatkd
      421 nqtvywnlla kclvgitcqa ekygmklchv sllrkvvfaw mdamkrghen esietfhevs
      481 vlvqklseis sgrdmrdiis hveefcvdts latssllllk civchvrpel lsanqrlvel
      541 ltgahcrcpt qlvaqclagy vnklpdadle ktlgciqpsl epdwtllkte lllwvikgll
      601 lrgypnlapy tkllkellkd eklgrhaakg fqqilqplvl tmdghcivkl mhpqrffvet
      661 vdfliegfns vtaeavkdnf lmalccqlay vpkpvvntyi dkilpilvta lssaeesvva
      721 ecvlpclten vglmashlds vleqllrlak kpfpmdvrrs alqcllrlst ikenvmlykq
      781 evlqgltpcl adrkrivrqv aaqasslwhm vgepsgv
//
LOCUS       XP_077501953             735 aa            linear   INV 25-NOV-2025
DEFINITION  lateral signaling target protein 2 homolog [Amblyomma americanum].
ACCESSION   XP_077501953
VERSION     XP_077501953.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645827.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..735
                     /product="lateral signaling target protein 2 homolog"
                     /calculated_mol_wt=82780
     Region          659..721
                     /region_name="FYVE_like_SF"
                     /note="FYVE domain like superfamily; cl28890"
                     /db_xref="CDD:333710"
     Site            order(682..687,692,714)
                     /site_type="other"
                     /note="phosphatidylinositol 3-phosphate binding site
                     [chemical binding]"
                     /db_xref="CDD:277249"
     CDS             1..735
                     /gene="LOC144112973"
                     /coded_by="XM_077645827.1:202..2409"
                     /db_xref="GeneID:144112973"
ORIGIN      
        1 mmnslrkwfy rpkkednsal aqfywadqel nlvateldsf dgrkdpdrcs alinqlrvcq
       61 drvlgichdi mneaipdtra drdfrakfpd dvlqenmsgl lwfgaeclaa gsnimnreve
      121 ssqmrplara ltrsldnvrc llreqclrnp heypeklkea lktfdrlfae felsyvsamv
      181 pvksakeyhl qqqvvvlfse tlqraikvkl vpqemvdtyd palmftiprl aivaglllfp
      241 dgplnvdkds sslselfrpf ysllckirel lwtlsreelw llektlcqle eprtsvtseq
      301 csfgvddyaa ldsdvyinlf yrnynsckqf iedfynhnfa apsesapilq fqkrirslgl
      361 eskelpavkr csplapkvva asqspslnat lsplckqpsa adsessesyd sddsvlsesw
      421 wplfsdtlqa gasggqpveq pqqqprqspc fsrpskapsf sdrmpvvpqi rrlqneehsl
      481 pspcsscksl sslssaewec msyqstdtss ynsdchddee ialalqavei afrnearsrf
      541 kssddlihrl fvgisgvadq lqtnfagemr nilkcvfemn ssskqdspak aleaespaer
      601 sgvaertlfs lsfldavere eegeqrgssg rrsvdsaqev lpddlslels sesghggnpv
      661 wmpdeltanc mdcnahftll rrrhhcrrcg kifcsrcsah sitlpryghy kpvrvcnscf
      721 icrvtsvvee lvhgc
//
LOCUS       XP_077501954             230 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin domain-containing protein 1 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501954
VERSION     XP_077501954.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645828.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..230
                     /product="ubiquitin domain-containing protein 1 isoform
                     X1"
                     /calculated_mol_wt=25089
     Region          34..136
                     /region_name="UBD"
                     /note="Ubiquitin-binding domain; pfam16455"
                     /db_xref="CDD:465122"
     Region          157..226
                     /region_name="Ubl_UBTD"
                     /note="ubiquitin-like (Ubl) domain found in ubiquitin
                     domain-containing proteins UBTD1, UBTD2, and similar
                     proteins; cd01794"
                     /db_xref="CDD:340492"
     CDS             1..230
                     /gene="LOC144112975"
                     /coded_by="XM_077645828.1:146..838"
                     /db_xref="GeneID:144112975"
ORIGIN      
        1 mggcigtrns rpssgiestd etnlqqtvsi gknqplrhek prwksdvplm egqlrskrde
       61 fwdtapafeg rkeiwdalka aayaaetndf tlaqaiidga nislpngtll dcydelgnry
      121 qlpvyclsap gnlvedasda espspesepc agheillklr lsttgkdtkm avrtgetila
      181 akrrledleg ipashqrwff ggkllsdrtr vdeaklqmgf vvqvavatps
//
LOCUS       XP_077501955             227 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin domain-containing protein 1 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501955
VERSION     XP_077501955.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645829.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..227
                     /product="ubiquitin domain-containing protein 1 isoform
                     X2"
                     /calculated_mol_wt=24790
     Region          31..133
                     /region_name="UBD"
                     /note="Ubiquitin-binding domain; pfam16455"
                     /db_xref="CDD:465122"
     Region          154..223
                     /region_name="Ubl_UBTD"
                     /note="ubiquitin-like (Ubl) domain found in ubiquitin
                     domain-containing proteins UBTD1, UBTD2, and similar
                     proteins; cd01794"
                     /db_xref="CDD:340492"
     CDS             1..227
                     /gene="LOC144112975"
                     /coded_by="XM_077645829.1:146..829"
                     /db_xref="GeneID:144112975"
ORIGIN      
        1 mggcigtrns rpssgiestd etnlqqtgkn qplrhekprw ksdvplmegq lrskrdefwd
       61 tapafegrke iwdalkaaay aaetndftla qaiidganis lpngtlldcy delgnryqlp
      121 vyclsapgnl vedasdaesp spesepcagh eillklrlst tgkdtkmavr tgetilaakr
      181 rledlegipa shqrwffggk llsdrtrvde aklqmgfvvq vavatps
//
LOCUS       XP_077501956             170 aa            linear   INV 25-NOV-2025
DEFINITION  mediator complex subunit 28 [Amblyomma americanum].
ACCESSION   XP_077501956
VERSION     XP_077501956.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645830.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..170
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..170
                     /product="mediator complex subunit 28"
                     /calculated_mol_wt=18934
     Region          6..106
                     /region_name="Med28"
                     /note="Mediator complex subunit 28; pfam11594"
                     /db_xref="CDD:463302"
     CDS             1..170
                     /gene="MED28"
                     /coded_by="XM_077645830.1:332..844"
                     /db_xref="GeneID:144112977"
ORIGIN      
        1 massnhivdd fensfqacla avtnpdyfyv rdseevktgv eqtvqrfldv akqmesfflq
       61 krlvlsaqks eqivvednte lknelarkeq llqkynekil ywqsllndts ntpgqqpqpq
      121 qqappgstpq gmptqgqqsm pmggsaqglp gplaylertt snigmpdprr
//
LOCUS       XP_077501957             170 aa            linear   INV 25-NOV-2025
DEFINITION  mediator complex subunit 28 [Amblyomma americanum].
ACCESSION   XP_077501957
VERSION     XP_077501957.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645831.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..170
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..170
                     /product="mediator complex subunit 28"
                     /calculated_mol_wt=18934
     Region          6..106
                     /region_name="Med28"
                     /note="Mediator complex subunit 28; pfam11594"
                     /db_xref="CDD:463302"
     CDS             1..170
                     /gene="MED28"
                     /coded_by="XM_077645831.1:341..853"
                     /db_xref="GeneID:144112977"
ORIGIN      
        1 massnhivdd fensfqacla avtnpdyfyv rdseevktgv eqtvqrfldv akqmesfflq
       61 krlvlsaqks eqivvednte lknelarkeq llqkynekil ywqsllndts ntpgqqpqpq
      121 qqappgstpq gmptqgqqsm pmggsaqglp gplaylertt snigmpdprr
//
LOCUS       XP_077501958             456 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112979 [Amblyomma americanum].
ACCESSION   XP_077501958
VERSION     XP_077501958.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645832.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..456
                     /product="uncharacterized protein LOC144112979"
                     /calculated_mol_wt=52506
     Region          84..178
                     /region_name="MULE"
                     /note="MULE transposase domain; pfam10551"
                     /db_xref="CDD:463145"
     CDS             1..456
                     /gene="LOC144112979"
                     /coded_by="XM_077645832.1:836..2206"
                     /db_xref="GeneID:144112979"
ORIGIN      
        1 mqlvttkdlr niadkfrigk eeqrdpddfk svdlwveemr aenespllyy mkkgdpnshd
       61 gdfelalmtf pqrellsklg teklcidsth gtnkykfqlt tlltisedgs gipcayvisk
      121 rvnmgtmvrf feaikektnt vqcqafmsdd apvygnaweq vmgtlksrll cswhvlhnwg
      181 arlntvpnkd crdtirdmlh tlmkctdete fqqllpafls iyskeqqlnr lapkeleeld
      241 gfiryfekty akracqwayc yqqyiglttn nhlesmhktl kyshfggkin grldklikvl
      301 fyltsdklyn raialmkgga dhrksvlfgr hkvgielckt stervgdgqw hvksqslvnf
      361 khtvqrtsks csgcelhckq ceachnmftc tcvdhamhah mckhmhaval iqqyivlvml
      421 alqeaccifv craewaaklf yrelnfytrl aiqilg
//
LOCUS       XP_077501959             286 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112980 [Amblyomma americanum].
ACCESSION   XP_077501959
VERSION     XP_077501959.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645833.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..286
                     /product="uncharacterized protein LOC144112980"
                     /calculated_mol_wt=32653
     Region          <18..239
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          95..115
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(95,98,111,115)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          123..143
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          151..171
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(151,154,167,171)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          179..199
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(179,182,195,199)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(184,186,188,190..191,194..195,198,212,214,218..219,
                     222..223,226,240,242,244,246..247,250..251,254)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          207..227
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          235..255
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          263..279
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..286
                     /gene="LOC144112980"
                     /coded_by="XM_077645833.1:253..1113"
                     /db_xref="GeneID:144112980"
ORIGIN      
        1 mrlelqglqt wrtswaelsr svvmqtrhsa spgqdsshls hcmesappgm gsvvmqtrhs
       61 assgqdsshl shcmestpgm sshqgkpagk nlhecthcnr gfkqeshlvk hlrvhtnesp
      121 fhcdqchssf tqkcnlvahl rihtgerpyh cdqcgssfvf kknltehlrt htgerpyhcd
      181 hcdstftqkg ilvahlrtht gerpyrcdhc gssfskrsnl vahirthtge kpyqcqlcpm
      241 gftdrtslvr hvrshkgekr fqcdlcqqkf yrryilkhhk damhke
//
LOCUS       XP_077501960             286 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112980 [Amblyomma americanum].
ACCESSION   XP_077501960
VERSION     XP_077501960.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645834.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..286
                     /product="uncharacterized protein LOC144112980"
                     /calculated_mol_wt=32653
     Region          <18..239
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          95..115
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(95,98,111,115)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          123..143
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          151..171
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(151,154,167,171)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          179..199
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(179,182,195,199)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(184,186,188,190..191,194..195,198,212,214,218..219,
                     222..223,226,240,242,244,246..247,250..251,254)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          207..227
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          235..255
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          263..279
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..286
                     /gene="LOC144112980"
                     /coded_by="XM_077645834.1:357..1217"
                     /db_xref="GeneID:144112980"
ORIGIN      
        1 mrlelqglqt wrtswaelsr svvmqtrhsa spgqdsshls hcmesappgm gsvvmqtrhs
       61 assgqdsshl shcmestpgm sshqgkpagk nlhecthcnr gfkqeshlvk hlrvhtnesp
      121 fhcdqchssf tqkcnlvahl rihtgerpyh cdqcgssfvf kknltehlrt htgerpyhcd
      181 hcdstftqkg ilvahlrtht gerpyrcdhc gssfskrsnl vahirthtge kpyqcqlcpm
      241 gftdrtslvr hvrshkgekr fqcdlcqqkf yrryilkhhk damhke
//
LOCUS       XP_077501961             286 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112983 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501961
VERSION     XP_077501961.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645835.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..286
                     /product="uncharacterized protein LOC144112983 isoform X1"
                     /calculated_mol_wt=32649
     Region          <18..239
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          95..115
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(95,98,111,115)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          123..143
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          151..171
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(151,154,167,171)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          179..199
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(179,182,195,199)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(184,186,188,190..191,194..195,198,212,214,218..219,
                     222..223,226,240,242,244,246..247,250..251,254)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          207..227
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          235..255
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          263..279
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..286
                     /gene="LOC144112983"
                     /coded_by="XM_077645835.1:253..1113"
                     /db_xref="GeneID:144112983"
ORIGIN      
        1 mrlelqglqt wrtswaelsr svvmqtrhsa spgqdsshls hcmesappgm gsvvmqtrhs
       61 assgqdsshl shcmestpgm sshqgkpagk nlhecthcnr gfkqeshlvk hlrvhtnesp
      121 fhcdqchssf tqkcnlvahl rihtgerpyh cdqcgssfvf kknltehlrt htgerpyhcd
      181 hcdstftqkg ilvahlrtht gerpyrcdhc gssfskrsnl vahirthtge kpyqcqlcpm
      241 gfpdrtslvr hvrshkgekr fqcdlcqqkf yrryilkhhk damhke
//
LOCUS       XP_077501962             116 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112983 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501962
VERSION     XP_077501962.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645836.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..116
                     /product="uncharacterized protein LOC144112983 isoform X2"
                     /calculated_mol_wt=13110
     CDS             1..116
                     /gene="LOC144112983"
                     /coded_by="XM_077645836.1:520..870"
                     /db_xref="GeneID:144112983"
ORIGIN      
        1 mrlelqglqt wrtswaelsr svvmqtrhsa spgqdsshls hcmesappgm gnhqgkpagk
       61 nlhecthcnr gfkqkadlvk hlsayrfcgh adqtqcifwp rffpsqplhg iyswhe
//
LOCUS       XP_077501963             276 aa            linear   INV 25-NOV-2025
DEFINITION  PR domain zinc finger protein 14-like [Amblyomma americanum].
ACCESSION   XP_077501963
VERSION     XP_077501963.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645837.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..276
                     /product="PR domain zinc finger protein 14-like"
                     /calculated_mol_wt=31019
     Region          <4..43
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Region          77..98
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(77,80,93,98)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          108..128
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(108,111,124,128)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(113,115,117,119..120,123..124,127,141,143,147..148,
                     151..152,155,169,171,173,175..176,179..180)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          135..156
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          136..156
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          148..171
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          164..180
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          193..209
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..276
                     /gene="LOC144112987"
                     /coded_by="XM_077645837.1:48..878"
                     /db_xref="GeneID:144112987"
ORIGIN      
        1 mndvnyspsk rtqnleaslv ngdiyyrtlr kvdpgeellv gystsftksl lgnttgrgal
       61 negdvsivpl hslflacgmc gelfttqykm emhtrqthwr skkgihqcth ctftstrkad
      121 iirherthvn krnsvcslcq krfsqpgnlk vhmmihtgek pyecgqcgkr freraharrh
      181 eevihsrryp lhcprcgaga advtrlrrht ckqlgttegk egfkrgrgrp rlmadgtspt
      241 vrprnttega plqggvkrgr grprkalaqd askprp
//
LOCUS       XP_077501964             198 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger X-chromosomal protein-like [Amblyomma americanum].
ACCESSION   XP_077501964
VERSION     XP_077501964.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645838.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..198
                     /product="zinc finger X-chromosomal protein-like"
                     /calculated_mol_wt=22857
     Region          2..20
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          30..50
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(30,33,46,50)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(35,37,39,41..42,45..46,49,63,65,69..70,73..74,77,91,
                     93,95,97..98,101..102)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          52..>112
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          58..78
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          70..93
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          86..102
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          115..131
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..198
                     /gene="LOC144112988"
                     /coded_by="XM_077645838.1:43..639"
                     /db_xref="GeneID:144112988"
ORIGIN      
        1 mcgdlfttqy kmemhtrwkh wrskkgihqc thctftstrk adiirherth vdkrnsvcnl
       61 cqkrfsrpdn lkvhmmihtr dkpyecsqcg krfrerahar rheevihsrr yplhcprcga
      121 gaedvtklrr htckqlgtte gkegfkrgrg rprlmadgta ptvrprntte gaplqggvkr
      181 grgqprkala qdaskprp
//
LOCUS       XP_077501965             197 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112989 [Amblyomma americanum].
ACCESSION   XP_077501965
VERSION     XP_077501965.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645839.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..197
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..197
                     /product="uncharacterized protein LOC144112989"
                     /calculated_mol_wt=21448
     Region          <4..38
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     Region          <73..>172
                     /region_name="PRK14959"
                     /note="DNA polymerase III subunits gamma and tau;
                     Provisional"
                     /db_xref="CDD:184923"
     CDS             1..197
                     /gene="LOC144112989"
                     /coded_by="XM_077645839.1:1..594"
                     /db_xref="GeneID:144112989"
ORIGIN      
        1 maaariqrwa hhlgayryll qyapgrqmln adalsrlpqq spqadpsalp gpkennydll
       61 plllsevyar nygagsiwtp chvettsgar mvtvetsdgv vqrhvcqvrp rpeaprdnpa
      121 vttatthcsa pdqatqlqhp etvspdegis arrtpngtgs pdlpapdipv aplntgvaqq
      181 tlrrstrehk pvqrfhf
//
LOCUS       XP_077501967             159 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112991 [Amblyomma americanum].
ACCESSION   XP_077501967
VERSION     XP_077501967.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645841.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..159
                     /product="uncharacterized protein LOC144112991"
                     /calculated_mol_wt=17181
     Region          85..141
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..159
                     /gene="LOC144112991"
                     /coded_by="XM_077645841.1:3..482"
                     /db_xref="GeneID:144112991"
ORIGIN      
        1 mhsetpatta lppapsdqgp lsagltprga afsieallgl gpaspdsstp apssnsescg
       61 gtegrrrpda csggrrqgss eprpkrvrti ftadqlerle aefqqqqymv gperlylaaa
      121 lrlteaqvkv wfqnrrikwr kqhldmqrla haasklppl
//
LOCUS       XP_077501968             694 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112993 [Amblyomma americanum].
ACCESSION   XP_077501968
VERSION     XP_077501968.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645842.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..694
                     /product="uncharacterized protein LOC144112993"
                     /calculated_mol_wt=79537
     Region          23..44
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          67..83
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          322..416
                     /region_name="MULE"
                     /note="MULE transposase domain; pfam10551"
                     /db_xref="CDD:463145"
     CDS             1..694
                     /gene="LOC144112993"
                     /coded_by="XM_077645842.1:41..2125"
                     /db_xref="GeneID:144112993"
ORIGIN      
        1 mqtdvsagaa sattgknser kwckfcdkaf sntsnlrkhv knvhgegalq hvkdeealtm
       61 kvrsfscdlc pkhfvilrdl rrhhvdehnf eeevenlvfd sntefctwkd geesrtcchf
      121 iastgrkesa ssvfkrylhc hhsgthkelp ieqrqrqkkg qgscrigkhc satlevtekd
      181 skvyvvfqkk hyghfnevch qrlsqdearv lagrlaegvp aktvvhnira dtsgssprmq
      241 lvttkdlrni adkfrigkee qrdpddfksv dlwveemrae nespllyymk kgdpnshdgd
      301 felalmtfpq rellsklgte klcidsthgt nkykfqlttl ltisedgsgi pcayviskrv
      361 nmgtmvrffe aikektntvq cqafmsddap vygnaweqvm gtlksrllcs whvlhnwgar
      421 lntvpnkdcr dtirdmlhtl mkctdetefq qllpaflsiy skeqqlnrla pkeleeldgf
      481 iryfektyak racqwaycyr qyiglttnnh lesmhktlky shfggkingr ldklikvlfy
      541 ltsdklynra ialmkggadh rksvlfgrhk vgielcktst ervgdgqwhv ksqslvnfkh
      601 tvqrtskscs gcelhckqce achnmftctc vdhamhahmc khmhavaliq qyivlvmlal
      661 qeaccifvcr aewaaklfyr elnfytrlai qilg
//
LOCUS       XP_077501969             146 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112994 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501969
VERSION     XP_077501969.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645843.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..146
                     /product="uncharacterized protein LOC144112994 isoform X1"
                     /calculated_mol_wt=16233
     CDS             1..146
                     /gene="LOC144112994"
                     /coded_by="XM_077645843.1:59..499"
                     /db_xref="GeneID:144112994"
ORIGIN      
        1 mrlelqglqt wrtswaelsr svvmqtrhsa spgqdsshls hcmesappgm gnhqgkpagk
       61 nlhecthcnr gfkqkadlvk hlsayrpllk sqcihgsrgr nlqyrtgels mtpllrahsl
      121 edcmcsdlsa lciqsewnqn gfhviv
//
LOCUS       XP_077501970             116 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112994 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501970
VERSION     XP_077501970.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645844.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..116
                     /product="uncharacterized protein LOC144112994 isoform X2"
                     /calculated_mol_wt=13110
     CDS             1..116
                     /gene="LOC144112994"
                     /coded_by="XM_077645844.1:44..394"
                     /db_xref="GeneID:144112994"
ORIGIN      
        1 mrlelqglqt wrtswaelsr svvmqtrhsa spgqdsshls hcmesappgm gnhqgkpagk
       61 nlhecthcnr gfkqkadlvk hlsayrfcgh adqtqcifwp rffpsqplhg iyswhe
//
LOCUS       XP_077501971             101 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112994 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501971
VERSION     XP_077501971.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645845.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..101
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..101
                     /product="uncharacterized protein LOC144112994 isoform X3"
                     /calculated_mol_wt=11239
     CDS             1..101
                     /gene="LOC144112994"
                     /coded_by="XM_077645845.1:236..541"
                     /db_xref="GeneID:144112994"
ORIGIN      
        1 manflgrtqq vcghanqtqc iswprffpsq plhgicsswh gpllksqcih gsrgrnlqyr
       61 tgelsmtpll rahsledcmc sdlsalciqs ewnqngfhvi v
//
LOCUS       XP_077501972             101 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112994 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501972
VERSION     XP_077501972.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645846.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..101
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..101
                     /product="uncharacterized protein LOC144112994 isoform X3"
                     /calculated_mol_wt=11239
     CDS             1..101
                     /gene="LOC144112994"
                     /coded_by="XM_077645846.1:324..629"
                     /db_xref="GeneID:144112994"
ORIGIN      
        1 manflgrtqq vcghanqtqc iswprffpsq plhgicsswh gpllksqcih gsrgrnlqyr
       61 tgelsmtpll rahsledcmc sdlsalciqs ewnqngfhvi v
//
LOCUS       XP_077501973             101 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112994 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501973
VERSION     XP_077501973.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645847.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..101
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..101
                     /product="uncharacterized protein LOC144112994 isoform X3"
                     /calculated_mol_wt=11239
     CDS             1..101
                     /gene="LOC144112994"
                     /coded_by="XM_077645847.1:195..500"
                     /db_xref="GeneID:144112994"
ORIGIN      
        1 manflgrtqq vcghanqtqc iswprffpsq plhgicsswh gpllksqcih gsrgrnlqyr
       61 tgelsmtpll rahsledcmc sdlsalciqs ewnqngfhvi v
//
LOCUS       XP_077501974              98 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112994 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077501974
VERSION     XP_077501974.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645848.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..98
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..98
                     /product="uncharacterized protein LOC144112994 isoform X4"
                     /calculated_mol_wt=10928
     CDS             1..98
                     /gene="LOC144112994"
                     /coded_by="XM_077645848.1:60..356"
                     /db_xref="GeneID:144112994"
ORIGIN      
        1 mrlelqglqt wrtswaelsr svvmqtrhsa spgqdsshls hcmesappgm gnhqgkpagk
       61 nlhecthcnr gfkqkadlvk hlsayrhgnq cyedrvya
//
LOCUS       XP_077501975             286 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112997 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501975
VERSION     XP_077501975.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645849.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..286
                     /product="uncharacterized protein LOC144112997 isoform X1"
                     /calculated_mol_wt=32649
     Region          <18..239
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          95..115
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(95,98,111,115)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          123..143
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          151..171
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(151,154,167,171)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          179..199
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(179,182,195,199)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(184,186,188,190..191,194..195,198,212,214,218..219,
                     222..223,226,240,242,244,246..247,250..251,254)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          207..227
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          235..255
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          263..279
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..286
                     /gene="LOC144112997"
                     /coded_by="XM_077645849.1:2055..2915"
                     /db_xref="GeneID:144112997"
ORIGIN      
        1 mrlelqglqt wrtswaelsr svvmqtrhsa spgqdsshls hcmesappgm gsvvmqtrhs
       61 assgqdsshl shcmestpgm sshqgkpagk nlhecthcnr gfkqeshlvk hlrvhtnesp
      121 fhcdqchssf tqkcnlvahl rihtgerpyh cdqcgssfvf kknltehlrt htgerpyhcd
      181 hcdstftqkg ilvahlrtht gerpyrcdhc gssfskrsnl vahirthtge kpyqcqlcpm
      241 gfpdrtslvr hvrshkgekr fqcdlcqqkf yrryilkhhk damhke
//
LOCUS       XP_077501976             286 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112997 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501976
VERSION     XP_077501976.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645850.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..286
                     /product="uncharacterized protein LOC144112997 isoform X1"
                     /calculated_mol_wt=32649
     Region          <18..239
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          95..115
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(95,98,111,115)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          123..143
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          151..171
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(151,154,167,171)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          179..199
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(179,182,195,199)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(184,186,188,190..191,194..195,198,212,214,218..219,
                     222..223,226,240,242,244,246..247,250..251,254)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          207..227
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          235..255
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          263..279
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..286
                     /gene="LOC144112997"
                     /coded_by="XM_077645850.1:1720..2580"
                     /db_xref="GeneID:144112997"
ORIGIN      
        1 mrlelqglqt wrtswaelsr svvmqtrhsa spgqdsshls hcmesappgm gsvvmqtrhs
       61 assgqdsshl shcmestpgm sshqgkpagk nlhecthcnr gfkqeshlvk hlrvhtnesp
      121 fhcdqchssf tqkcnlvahl rihtgerpyh cdqcgssfvf kknltehlrt htgerpyhcd
      181 hcdstftqkg ilvahlrtht gerpyrcdhc gssfskrsnl vahirthtge kpyqcqlcpm
      241 gfpdrtslvr hvrshkgekr fqcdlcqqkf yrryilkhhk damhke
//
LOCUS       XP_077501977             286 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112997 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501977
VERSION     XP_077501977.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645851.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..286
                     /product="uncharacterized protein LOC144112997 isoform X1"
                     /calculated_mol_wt=32649
     Region          <18..239
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          95..115
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(95,98,111,115)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          123..143
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          151..171
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(151,154,167,171)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          179..199
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(179,182,195,199)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(184,186,188,190..191,194..195,198,212,214,218..219,
                     222..223,226,240,242,244,246..247,250..251,254)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          207..227
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          235..255
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          263..279
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..286
                     /gene="LOC144112997"
                     /coded_by="XM_077645851.1:701..1561"
                     /db_xref="GeneID:144112997"
ORIGIN      
        1 mrlelqglqt wrtswaelsr svvmqtrhsa spgqdsshls hcmesappgm gsvvmqtrhs
       61 assgqdsshl shcmestpgm sshqgkpagk nlhecthcnr gfkqeshlvk hlrvhtnesp
      121 fhcdqchssf tqkcnlvahl rihtgerpyh cdqcgssfvf kknltehlrt htgerpyhcd
      181 hcdstftqkg ilvahlrtht gerpyrcdhc gssfskrsnl vahirthtge kpyqcqlcpm
      241 gfpdrtslvr hvrshkgekr fqcdlcqqkf yrryilkhhk damhke
//
LOCUS       XP_077501978             286 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112997 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077501978
VERSION     XP_077501978.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645852.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..286
                     /product="uncharacterized protein LOC144112997 isoform X1"
                     /calculated_mol_wt=32649
     Region          <18..239
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          95..115
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(95,98,111,115)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          123..143
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          151..171
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(151,154,167,171)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          179..199
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(179,182,195,199)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(184,186,188,190..191,194..195,198,212,214,218..219,
                     222..223,226,240,242,244,246..247,250..251,254)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          207..227
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          235..255
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          263..279
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..286
                     /gene="LOC144112997"
                     /coded_by="XM_077645852.1:262..1122"
                     /db_xref="GeneID:144112997"
ORIGIN      
        1 mrlelqglqt wrtswaelsr svvmqtrhsa spgqdsshls hcmesappgm gsvvmqtrhs
       61 assgqdsshl shcmestpgm sshqgkpagk nlhecthcnr gfkqeshlvk hlrvhtnesp
      121 fhcdqchssf tqkcnlvahl rihtgerpyh cdqcgssfvf kknltehlrt htgerpyhcd
      181 hcdstftqkg ilvahlrtht gerpyrcdhc gssfskrsnl vahirthtge kpyqcqlcpm
      241 gfpdrtslvr hvrshkgekr fqcdlcqqkf yrryilkhhk damhke
//
LOCUS       XP_077501979             146 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112997 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077501979
VERSION     XP_077501979.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645853.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..146
                     /product="uncharacterized protein LOC144112997 isoform X2"
                     /calculated_mol_wt=16233
     CDS             1..146
                     /gene="LOC144112997"
                     /coded_by="XM_077645853.1:2061..2501"
                     /db_xref="GeneID:144112997"
ORIGIN      
        1 mrlelqglqt wrtswaelsr svvmqtrhsa spgqdsshls hcmesappgm gnhqgkpagk
       61 nlhecthcnr gfkqkadlvk hlsayrpllk sqcihgsrgr nlqyrtgels mtpllrahsl
      121 edcmcsdlsa lciqsewnqn gfhviv
//
LOCUS       XP_077501980             116 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112997 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077501980
VERSION     XP_077501980.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645854.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..116
                     /product="uncharacterized protein LOC144112997 isoform X3"
                     /calculated_mol_wt=13110
     CDS             1..116
                     /gene="LOC144112997"
                     /coded_by="XM_077645854.1:2061..2411"
                     /db_xref="GeneID:144112997"
ORIGIN      
        1 mrlelqglqt wrtswaelsr svvmqtrhsa spgqdsshls hcmesappgm gnhqgkpagk
       61 nlhecthcnr gfkqkadlvk hlsayrfcgh adqtqcifwp rffpsqplhg iyswhe
//
LOCUS       XP_077501981              98 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144112997 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077501981
VERSION     XP_077501981.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645855.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..98
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..98
                     /product="uncharacterized protein LOC144112997 isoform X4"
                     /calculated_mol_wt=10928
     CDS             1..98
                     /gene="LOC144112997"
                     /coded_by="XM_077645855.1:2061..2357"
                     /db_xref="GeneID:144112997"
ORIGIN      
        1 mrlelqglqt wrtswaelsr svvmqtrhsa spgqdsshls hcmesappgm gnhqgkpagk
       61 nlhecthcnr gfkqkadlvk hlsayrhgnq cyedrvya
//
LOCUS       XP_077501982             640 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-L-iduronidase isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501982
VERSION     XP_077501982.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645856.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..640
                     /product="alpha-L-iduronidase isoform X1"
                     /calculated_mol_wt=69943
     Region          44..513
                     /region_name="Glyco_hydro"
                     /note="Glycosyl hydrolases; cl23725"
                     /db_xref="CDD:474034"
     CDS             1..640
                     /gene="Idua"
                     /coded_by="XM_077645856.1:91..2013"
                     /db_xref="GeneID:144113001"
ORIGIN      
        1 mlaagvillf ceisfaetlc ldsdisyvrk ldaakqtsie sfqvfvnasd vvgvmkpfwk
       61 stgfcppeph ndpefflgdd mqqnliyiaa ipgygttqvr mhwlldivna satleldfsr
      121 tfnfslldtl ldrlralglk pglelmgnpf grpldfeqsa dldlwkelvt algnhylaya
      181 erygedyvaq wnfeswnepd hkgfgrnfte qgicnyydac seglreaspl lrlggpaenl
      241 asshrsplaw alldhcerga rldfismhqk gnattagive gelnalsrir drcpslsakp
      301 cinneadpik nwaepypwra datyaamlar lvhahmtgag tcgslssdng flsypphpfs
      361 qrtqlarfrm nltadphvlf vrkpvhaaqa mlgklasrvv vatvrhqaqd dagepapqlg
      421 viatasgshs lsallyhsne svwqpgveak vslraqldws tgaswavyqv dnqltnpyaa
      481 wvrqgcppfp salqlddira seeprlvafg tsgagaelsv elslplpsaa lvlacgahgp
      541 rpatprdvrl hrvsrnrtll hwrqprdgct sgfrllfsaa aagpytplte rrpllplhlv
      601 dceltpeaga crgpgfvrvg alslaggrsa lsrpvpwrpl
//
LOCUS       XP_077501983             637 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-L-iduronidase isoform X2 [Amblyomma americanum].
ACCESSION   XP_077501983
VERSION     XP_077501983.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645857.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..637
                     /product="alpha-L-iduronidase isoform X2"
                     /calculated_mol_wt=69637
     Region          44..510
                     /region_name="Glyco_hydro"
                     /note="Glycosyl hydrolases; cl23725"
                     /db_xref="CDD:474034"
     CDS             1..637
                     /gene="Idua"
                     /coded_by="XM_077645857.1:90..2003"
                     /db_xref="GeneID:144113001"
ORIGIN      
        1 mlaagvillf ceisfaetlc ldsdisyvrk ldaakqtsie sfqvfvnasd vvgvmkpfwk
       61 stgfcppeph ndpefflgdd mqqnliyiaa ipgygttqvr mhwlldivna satleldfsr
      121 tfnfslldtl ldrlralglk pglelmgnpf grpldfeqsa dldlwkelvt algnhylery
      181 gedyvaqwnf eswnepdhkg fgrnfteqgi cnyydacseg lreaspllrl ggpaenlass
      241 hrsplawall dhcergarld fismhqkgna ttagivegel nalsrirdrc pslsakpcin
      301 neadpiknwa epypwradat yaamlarlvh ahmtgagtcg slssdngfls ypphpfsqrt
      361 qlarfrmnlt adphvlfvrk pvhaaqamlg klasrvvvat vrhqaqddag epapqlgvia
      421 tasgshslsa llyhsnesvw qpgveakvsl raqldwstga swavyqvdnq ltnpyaawvr
      481 qgcppfpsal qlddirasee prlvafgtsg agaelsvels lplpsaalvl acgahgprpa
      541 tprdvrlhrv srnrtllhwr qprdgctsgf rllfsaaaag pytplterrp llplhlvdce
      601 ltpeagacrg pgfvrvgals laggrsalsr pvpwrpl
//
LOCUS       XP_077501984             371 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4B chain-like [Amblyomma americanum].
ACCESSION   XP_077501984
VERSION     XP_077501984.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645858.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..371
                     /product="tubulin beta-4B chain-like"
                     /calculated_mol_wt=41607
     Region          1..371
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..371
                     /gene="LOC144113002"
                     /coded_by="XM_077645858.1:941..2056"
                     /db_xref="GeneID:144113002"
ORIGIN      
        1 mdavrsgpfg qlfrpdnfvf gqsgagnnwa kghytegael vdsvldvvrk eaescdclqg
       61 fqlthslggg tgsgmgtlli skireeypdr fmntysvvps pkvsdtvvep ynatlsmhql
      121 ventdetyci dnealydicf rtlklttpty gdlnhlvsat msgvttclrf pgqlnadlrk
      181 lavnmvpfpr lhffltgfap ltsrgcqqyr altvpeltqq mfdaknmmaa cdprhsrylt
      241 vaavfrgrms mrevddqmln iqnkdssyfv ewipnnvkta vcdipprglk msatfianst
      301 aiqelfkris eqftamfrrk aflhwytgeg mdelefteae sdlnelvsey qqyqeataed
      361 egefeetqae a
//
LOCUS       XP_077501987             182 aa            linear   INV 25-NOV-2025
DEFINITION  homeobox protein SAX-1-like [Amblyomma americanum].
ACCESSION   XP_077501987
VERSION     XP_077501987.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645861.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..182
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..182
                     /product="homeobox protein SAX-1-like"
                     /calculated_mol_wt=20100
     Region          82..138
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..182
                     /gene="LOC144113003"
                     /coded_by="XM_077645861.1:1..549"
                     /db_xref="GeneID:144113003"
ORIGIN      
        1 mqqpqappae pcsspkqpta fsvddildpa kftgtrfrwr plleasadws sdergaspak
       61 ngklsssscs kkkpepkagk prrartafty eqlvalenkf kttrylsvce rlnlalslrl
      121 tetqvkiwfq nrrtkwkkqn pgldanspal pasppspfsa gslygpylap ysvfpqppff
      181 ps
//
LOCUS       XP_077501988             232 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113004 [Amblyomma americanum].
ACCESSION   XP_077501988
VERSION     XP_077501988.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645862.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..232
                     /product="uncharacterized protein LOC144113004"
                     /calculated_mol_wt=25654
     CDS             1..232
                     /gene="LOC144113004"
                     /coded_by="XM_077645862.1:926..1624"
                     /db_xref="GeneID:144113004"
ORIGIN      
        1 mddfmrlagv vrerikchaf edghtqlvsl neycwnaigl yldlddiqew ledlnktadg
       61 hssiwtsedn gcppprlpph cypvavpcgs nllpgndrrp swcsclmpll skqppprrwk
      121 rwytassvtl lpcsstlhaa ptchlvttdg argvsclapl lskqtprhrk rrypasratl
      181 lpcsstlrsq pavwkrptal vvqlpdgpav qtalyappet gqssvhrrta tl
//
LOCUS       XP_077501989             232 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113004 [Amblyomma americanum].
ACCESSION   XP_077501989
VERSION     XP_077501989.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645863.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..232
                     /product="uncharacterized protein LOC144113004"
                     /calculated_mol_wt=25654
     CDS             1..232
                     /gene="LOC144113004"
                     /coded_by="XM_077645863.1:926..1624"
                     /db_xref="GeneID:144113004"
ORIGIN      
        1 mddfmrlagv vrerikchaf edghtqlvsl neycwnaigl yldlddiqew ledlnktadg
       61 hssiwtsedn gcppprlpph cypvavpcgs nllpgndrrp swcsclmpll skqppprrwk
      121 rwytassvtl lpcsstlhaa ptchlvttdg argvsclapl lskqtprhrk rrypasratl
      181 lpcsstlrsq pavwkrptal vvqlpdgpav qtalyappet gqssvhrrta tl
//
LOCUS       XP_077501990             232 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113005 [Amblyomma americanum].
ACCESSION   XP_077501990
VERSION     XP_077501990.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645864.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..232
                     /product="uncharacterized protein LOC144113005"
                     /calculated_mol_wt=25632
     CDS             1..232
                     /gene="LOC144113005"
                     /coded_by="XM_077645864.1:1794..2492"
                     /db_xref="GeneID:144113005"
ORIGIN      
        1 mddfmrlagv vgerikchaf edghtqlvsl neycwsaigl yldlddiqew lddlnktadg
       61 hssiwtsedn gcppprlpph cypvavpcgs nllpgndrrp swcsclmpll skqppprrwk
      121 rwytassvtl lpcsstlhaa ptchlvttdg argvsylapl lskqtprhrk rrypasraml
      181 lpcsstlrsq pavwkrptal vvqlpdgpav qtalyvppet gqssvhrrta tl
//
LOCUS       XP_077501991             232 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113005 [Amblyomma americanum].
ACCESSION   XP_077501991
VERSION     XP_077501991.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645865.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..232
                     /product="uncharacterized protein LOC144113005"
                     /calculated_mol_wt=25632
     CDS             1..232
                     /gene="LOC144113005"
                     /coded_by="XM_077645865.1:1588..2286"
                     /db_xref="GeneID:144113005"
ORIGIN      
        1 mddfmrlagv vgerikchaf edghtqlvsl neycwsaigl yldlddiqew lddlnktadg
       61 hssiwtsedn gcppprlpph cypvavpcgs nllpgndrrp swcsclmpll skqppprrwk
      121 rwytassvtl lpcsstlhaa ptchlvttdg argvsylapl lskqtprhrk rrypasraml
      181 lpcsstlrsq pavwkrptal vvqlpdgpav qtalyvppet gqssvhrrta tl
//
LOCUS       XP_077501992             425 aa            linear   INV 25-NOV-2025
DEFINITION  sil1 nucleotide exchange factor isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501992
VERSION     XP_077501992.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645866.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..425
                     /product="sil1 nucleotide exchange factor isoform X1"
                     /calculated_mol_wt=47333
     Region          196..221
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Site            order(212,216,220,255,259,263,299,303,307)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293788"
     Region          229..266
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          272..308
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     CDS             1..425
                     /gene="Sil1"
                     /coded_by="XM_077645866.1:204..1481"
                     /db_xref="GeneID:144113007"
ORIGIN      
        1 mlqkrvlcva aallvftknt ccqqqsisvv agqdqsqkkd dkkskepeqs prkefqptde
       61 wkqvqpgeti paglhvrmnl atgqteaklm desdskgtke sqssgshdnr lrleqakggp
      121 piklsekknl pwnmdelkkk vsslklqaks eseilqtlle syrnstevgk eallrdmefl
      181 vhqydravdf lnmggllaia pdlnstsdtv relvaftlgs alqgnpkvqk tvlghgllpq
      241 llrlvawdps trvrlrclfa lsclvrqlpe aqeallhhgg ltvlaglftw ssgsdklqlk
      301 avtllhdliv eqrlrheagq ptyseltqsi qlhgfctlvp kllhspdvdv qekvvqamta
      361 lantclgefq qhvpllqtll snyrrqadhe hlskevdqaa dyyegllhsv tqlldslqqk
      421 ykdel
//
LOCUS       XP_077501993             425 aa            linear   INV 25-NOV-2025
DEFINITION  sil1 nucleotide exchange factor isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501993
VERSION     XP_077501993.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645867.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..425
                     /product="sil1 nucleotide exchange factor isoform X1"
                     /calculated_mol_wt=47333
     Region          196..221
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Site            order(212,216,220,255,259,263,299,303,307)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293788"
     Region          229..266
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          272..308
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     CDS             1..425
                     /gene="Sil1"
                     /coded_by="XM_077645867.1:247..1524"
                     /db_xref="GeneID:144113007"
ORIGIN      
        1 mlqkrvlcva aallvftknt ccqqqsisvv agqdqsqkkd dkkskepeqs prkefqptde
       61 wkqvqpgeti paglhvrmnl atgqteaklm desdskgtke sqssgshdnr lrleqakggp
      121 piklsekknl pwnmdelkkk vsslklqaks eseilqtlle syrnstevgk eallrdmefl
      181 vhqydravdf lnmggllaia pdlnstsdtv relvaftlgs alqgnpkvqk tvlghgllpq
      241 llrlvawdps trvrlrclfa lsclvrqlpe aqeallhhgg ltvlaglftw ssgsdklqlk
      301 avtllhdliv eqrlrheagq ptyseltqsi qlhgfctlvp kllhspdvdv qekvvqamta
      361 lantclgefq qhvpllqtll snyrrqadhe hlskevdqaa dyyegllhsv tqlldslqqk
      421 ykdel
//
LOCUS       XP_077501994             425 aa            linear   INV 25-NOV-2025
DEFINITION  sil1 nucleotide exchange factor isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501994
VERSION     XP_077501994.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645868.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..425
                     /product="sil1 nucleotide exchange factor isoform X1"
                     /calculated_mol_wt=47333
     Region          196..221
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Site            order(212,216,220,255,259,263,299,303,307)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293788"
     Region          229..266
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          272..308
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     CDS             1..425
                     /gene="Sil1"
                     /coded_by="XM_077645868.1:131..1408"
                     /db_xref="GeneID:144113007"
ORIGIN      
        1 mlqkrvlcva aallvftknt ccqqqsisvv agqdqsqkkd dkkskepeqs prkefqptde
       61 wkqvqpgeti paglhvrmnl atgqteaklm desdskgtke sqssgshdnr lrleqakggp
      121 piklsekknl pwnmdelkkk vsslklqaks eseilqtlle syrnstevgk eallrdmefl
      181 vhqydravdf lnmggllaia pdlnstsdtv relvaftlgs alqgnpkvqk tvlghgllpq
      241 llrlvawdps trvrlrclfa lsclvrqlpe aqeallhhgg ltvlaglftw ssgsdklqlk
      301 avtllhdliv eqrlrheagq ptyseltqsi qlhgfctlvp kllhspdvdv qekvvqamta
      361 lantclgefq qhvpllqtll snyrrqadhe hlskevdqaa dyyegllhsv tqlldslqqk
      421 ykdel
//
LOCUS       XP_077501995             425 aa            linear   INV 25-NOV-2025
DEFINITION  sil1 nucleotide exchange factor isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501995
VERSION     XP_077501995.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645869.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..425
                     /product="sil1 nucleotide exchange factor isoform X1"
                     /calculated_mol_wt=47333
     Region          196..221
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Site            order(212,216,220,255,259,263,299,303,307)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293788"
     Region          229..266
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          272..308
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     CDS             1..425
                     /gene="Sil1"
                     /coded_by="XM_077645869.1:62..1339"
                     /db_xref="GeneID:144113007"
ORIGIN      
        1 mlqkrvlcva aallvftknt ccqqqsisvv agqdqsqkkd dkkskepeqs prkefqptde
       61 wkqvqpgeti paglhvrmnl atgqteaklm desdskgtke sqssgshdnr lrleqakggp
      121 piklsekknl pwnmdelkkk vsslklqaks eseilqtlle syrnstevgk eallrdmefl
      181 vhqydravdf lnmggllaia pdlnstsdtv relvaftlgs alqgnpkvqk tvlghgllpq
      241 llrlvawdps trvrlrclfa lsclvrqlpe aqeallhhgg ltvlaglftw ssgsdklqlk
      301 avtllhdliv eqrlrheagq ptyseltqsi qlhgfctlvp kllhspdvdv qekvvqamta
      361 lantclgefq qhvpllqtll snyrrqadhe hlskevdqaa dyyegllhsv tqlldslqqk
      421 ykdel
//
LOCUS       XP_077501996             425 aa            linear   INV 25-NOV-2025
DEFINITION  sil1 nucleotide exchange factor isoform X1 [Amblyomma americanum].
ACCESSION   XP_077501996
VERSION     XP_077501996.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645870.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..425
                     /product="sil1 nucleotide exchange factor isoform X1"
                     /calculated_mol_wt=47333
     Region          196..221
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Site            order(212,216,220,255,259,263,299,303,307)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293788"
     Region          229..266
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          272..308
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     CDS             1..425
                     /gene="Sil1"
                     /coded_by="XM_077645870.1:235..1512"
                     /db_xref="GeneID:144113007"
ORIGIN      
        1 mlqkrvlcva aallvftknt ccqqqsisvv agqdqsqkkd dkkskepeqs prkefqptde
       61 wkqvqpgeti paglhvrmnl atgqteaklm desdskgtke sqssgshdnr lrleqakggp
      121 piklsekknl pwnmdelkkk vsslklqaks eseilqtlle syrnstevgk eallrdmefl
      181 vhqydravdf lnmggllaia pdlnstsdtv relvaftlgs alqgnpkvqk tvlghgllpq
      241 llrlvawdps trvrlrclfa lsclvrqlpe aqeallhhgg ltvlaglftw ssgsdklqlk
      301 avtllhdliv eqrlrheagq ptyseltqsi qlhgfctlvp kllhspdvdv qekvvqamta
      361 lantclgefq qhvpllqtll snyrrqadhe hlskevdqaa dyyegllhsv tqlldslqqk
      421 ykdel
//
LOCUS       XP_077501997             340 aa            linear   INV 25-NOV-2025
DEFINITION  sil1 nucleotide exchange factor isoform X2 [Amblyomma americanum].
ACCESSION   XP_077501997
VERSION     XP_077501997.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645871.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..340
                     /product="sil1 nucleotide exchange factor isoform X2"
                     /calculated_mol_wt=37304
     Region          196..221
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Site            order(212,216,220,255,259,263,299,303,307)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293788"
     Region          229..266
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          272..308
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     CDS             1..340
                     /gene="Sil1"
                     /coded_by="XM_077645871.1:204..1226"
                     /db_xref="GeneID:144113007"
ORIGIN      
        1 mlqkrvlcva aallvftknt ccqqqsisvv agqdqsqkkd dkkskepeqs prkefqptde
       61 wkqvqpgeti paglhvrmnl atgqteaklm desdskgtke sqssgshdnr lrleqakggp
      121 piklsekknl pwnmdelkkk vsslklqaks eseilqtlle syrnstevgk eallrdmefl
      181 vhqydravdf lnmggllaia pdlnstsdtv relvaftlgs alqgnpkvqk tvlghgllpq
      241 llrlvawdps trvrlrclfa lsclvrqlpe aqeallhhgg ltvlaglftw ssgsdklqlk
      301 avtllhdliv eqrlrheagq ptysetgipt llaaadggcp
//
LOCUS       XP_077501999             440 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113009 [Amblyomma americanum].
ACCESSION   XP_077501999
VERSION     XP_077501999.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645873.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..440
                     /product="uncharacterized protein LOC144113009"
                     /calculated_mol_wt=50541
     Region          315..403
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..440
                     /gene="LOC144113009"
                     /coded_by="XM_077645873.1:257..1579"
                     /db_xref="GeneID:144113009"
ORIGIN      
        1 mysmemnnfv sctlreqdyf dllsgplled ddkdffepdl sfqlpnpeid fesdvacwvq
       61 qspvwgknlt evnsleeeve envkwvsevp gwikevpkie seeedevdvv svdddksiqs
      121 tepstptkss pqptpekspk ktegppvvss slldrmkaas akkrspiliq ppernrppak
      181 ndaatntapc paatpdhdyc taaksnskae nhsskhergv vrrvsrspsv trnyrsqkrs
      241 lsrrsrsprr rrsrsssssr ssscgssdgs vrqrsrssrr prdcrsswrp qhrhreprrf
      301 mpspprrrip dqkeerrviy vgnipegttr ldlrerfarf ghieevsvhf rdhgdnygfv
      361 tflrgadaye avehgnddpk lprfdlcfgg rrqfcrtnwa dldsqyekyy yiqrephvns
      421 ldfdsllqaa ktkiqghhry
//
LOCUS       XP_077502000             655 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113010 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502000
VERSION     XP_077502000.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645874.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..655
                     /product="uncharacterized protein LOC144113010 isoform X1"
                     /calculated_mol_wt=72259
     Region          24..65
                     /region_name="DnaJ"
                     /note="DnaJ domain or J-domain. DnaJ/Hsp40 (heat shock
                     protein 40) proteins are highly conserved and play crucial
                     roles in protein translation, folding, unfolding,
                     translocation, and degradation. They act primarily by
                     stimulating the ATPase activity of Hsp70s; cl02542"
                     /db_xref="CDD:413365"
     Site            order(40..42,51,54..55,58..59)
                     /site_type="other"
                     /note="HSP70 interaction site [polypeptide binding]"
                     /db_xref="CDD:99751"
     Region          255..>448
                     /region_name="3a0801s09"
                     /note="mitochondrial precursor proteins import receptor
                     (72 kDa mitochondrial outermembrane protein)
                     (mitochondrial import receptor for the ADP/ATP carrier)
                     (translocase of outermembrane tom70); TIGR00990"
                     /db_xref="CDD:273380"
     Region          355..>445
                     /region_name="TPR"
                     /note="Tetratricopeptide (TPR) repeat [General function
                     prediction only]; COG0457"
                     /db_xref="CDD:440225"
     Region          374..404
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          409..437
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          457..486
                     /region_name="UBA_like_SF"
                     /note="UBA domain-like superfamily; cl21463"
                     /db_xref="CDD:473871"
     Region          508..574
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cd00590"
                     /db_xref="CDD:409669"
     CDS             1..655
                     /gene="LOC144113010"
                     /coded_by="XM_077645874.1:276..2243"
                     /db_xref="GeneID:144113010"
ORIGIN      
        1 mivtkeeals ilklkdpsec tdpspqdiqk ryqklalkwh pskhhadrrs irkfdmlcrs
       61 yivltegrds idkcltsrem lnvfikafel edlgfnldva aqdecrrplv afadectgcr
      121 gqpsahgctq cnkppsaasr cnepellats altpaeirel lhlaawassp aftlfeepla
      181 aststewkkp lstehhvlpg vgtsakecvs tasvgtsaap vkllvteepk qneatvvpap
      241 saeqlpdkkt kkkasrktak rrkqrekaea kkssakydrs ssseemsted ekkkvadegl
      301 dpnsafvsma lsrktkgans asggvipkvs kksgkdvivr srrlaregcd kcnvgrhkea
      361 vdlftqaikl ypqdqsyfgn rsycysilgd ydkalkdaek aislqpqqak gyfrranaql
      421 glqkyseaae sfrkvleidp dcheakselh svcvfevmsm gftleeaews vtkgnndvqe
      481 ainllfgpda vkpdmklnpv nlegsqslwv gnikpevtek mlmslfrrfg dvhsirilhd
      541 rhcafinygn kvspgramda lqghllcgka ilirfpdhnf mdkpprsprs ggedqalaaa
      601 gatampkakp pgplknnecy fwrttgcayg ihcnfkhips nrgidlllds ngkci
//
LOCUS       XP_077502001             652 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113010 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502001
VERSION     XP_077502001.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645875.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..652
                     /product="uncharacterized protein LOC144113010 isoform X2"
                     /calculated_mol_wt=71824
     Region          24..65
                     /region_name="DnaJ"
                     /note="DnaJ domain or J-domain. DnaJ/Hsp40 (heat shock
                     protein 40) proteins are highly conserved and play crucial
                     roles in protein translation, folding, unfolding,
                     translocation, and degradation. They act primarily by
                     stimulating the ATPase activity of Hsp70s; cl02542"
                     /db_xref="CDD:413365"
     Site            order(40..42,51,54..55,58..59)
                     /site_type="other"
                     /note="HSP70 interaction site [polypeptide binding]"
                     /db_xref="CDD:99751"
     Region          352..>442
                     /region_name="TPR"
                     /note="Tetratricopeptide (TPR) repeat [General function
                     prediction only]; COG0457"
                     /db_xref="CDD:440225"
     Region          371..401
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          406..434
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          454..483
                     /region_name="UBA_like_SF"
                     /note="UBA domain-like superfamily; cl21463"
                     /db_xref="CDD:473871"
     Region          505..571
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cd00590"
                     /db_xref="CDD:409669"
     CDS             1..652
                     /gene="LOC144113010"
                     /coded_by="XM_077645875.1:133..2091"
                     /db_xref="GeneID:144113010"
ORIGIN      
        1 mivtkeeals ilklkdpsec tdpspqdiqk ryqklalkwh pskhhadrrs irkfdmlcrs
       61 yivltegrds idkcltsrem lnvfikafel edlgfnldva aqdecrrplv afadectgcr
      121 gqpsahgctq cnkppsaasr cnepellats altpaeirel lhlaawassp aftlfeepla
      181 aststewkkp lstehhvlpg vgtsakecvs tasvgtsaap vkllvteepk qneatvvpap
      241 saeqlpdkkt kkkasrktak rrkqrekaea kkssakssss eemstedekk kvadegldpn
      301 safvsmalsr ktkgansasg gvipkvskks gkdvivrsrr laregcdkcn vgrhkeavdl
      361 ftqaiklypq dqsyfgnrsy cysilgdydk alkdaekais lqpqqakgyf rranaqlglq
      421 kyseaaesfr kvleidpdch eakselhsvc vfevmsmgft leeaewsvtk gnndvqeain
      481 llfgpdavkp dmklnpvnle gsqslwvgni kpevtekmlm slfrrfgdvh sirilhdrhc
      541 afinygnkvs pgramdalqg hllcgkaili rfpdhnfmdk pprsprsgge dqalaaagat
      601 ampkakppgp lknnecyfwr ttgcaygihc nfkhipsnrg idllldsngk ci
//
LOCUS       XP_077502002             651 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113010 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502002
VERSION     XP_077502002.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645876.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..651
                     /product="uncharacterized protein LOC144113010 isoform X3"
                     /calculated_mol_wt=71837
     Region          11..61
                     /region_name="DnaJ"
                     /note="DnaJ domain or J-domain. DnaJ/Hsp40 (heat shock
                     protein 40) proteins are highly conserved and play crucial
                     roles in protein translation, folding, unfolding,
                     translocation, and degradation. They act primarily by
                     stimulating the ATPase activity of Hsp70s; cl02542"
                     /db_xref="CDD:413365"
     Site            order(36..38,47,50..51,54..55)
                     /site_type="other"
                     /note="HSP70 interaction site [polypeptide binding]"
                     /db_xref="CDD:99751"
     Region          251..>444
                     /region_name="3a0801s09"
                     /note="mitochondrial precursor proteins import receptor
                     (72 kDa mitochondrial outermembrane protein)
                     (mitochondrial import receptor for the ADP/ATP carrier)
                     (translocase of outermembrane tom70); TIGR00990"
                     /db_xref="CDD:273380"
     Region          351..>441
                     /region_name="TPR"
                     /note="Tetratricopeptide (TPR) repeat [General function
                     prediction only]; COG0457"
                     /db_xref="CDD:440225"
     Region          370..400
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          405..433
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          453..482
                     /region_name="UBA_like_SF"
                     /note="UBA domain-like superfamily; cl21463"
                     /db_xref="CDD:473871"
     Region          504..570
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cd00590"
                     /db_xref="CDD:409669"
     CDS             1..651
                     /gene="LOC144113010"
                     /coded_by="XM_077645876.1:290..2245"
                     /db_xref="GeneID:144113010"
ORIGIN      
        1 mivtkeeals ilklkdpnps pqdiqkryqk lalkwhpskh hadrrsirkf dmlcrsyivl
       61 tegrdsidkc ltsremlnvf ikafeledlg fnldvaaqde crrplvafad ectgcrgqps
      121 ahgctqcnkp psaasrcnep ellatsaltp aeirellhla awasspaftl feeplaasts
      181 tewkkplste hhvlpgvgts akecvstasv gtsaapvkll vteepkqnea tvvpapsaeq
      241 lpdkktkkka srktakrrkq rekaeakkss akydrsssse emstedekkk vadegldpns
      301 afvsmalsrk tkgansasgg vipkvskksg kdvivrsrrl aregcdkcnv grhkeavdlf
      361 tqaiklypqd qsyfgnrsyc ysilgdydka lkdaekaisl qpqqakgyfr ranaqlglqk
      421 yseaaesfrk vleidpdche akselhsvcv fevmsmgftl eeaewsvtkg nndvqeainl
      481 lfgpdavkpd mklnpvnleg sqslwvgnik pevtekmlms lfrrfgdvhs irilhdrhca
      541 finygnkvsp gramdalqgh llcgkailir fpdhnfmdkp prsprsgged qalaaagata
      601 mpkakppgpl knnecyfwrt tgcaygihcn fkhipsnrgi dllldsngkc i
//
LOCUS       XP_077502003             643 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113010 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077502003
VERSION     XP_077502003.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645877.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..643
                     /product="uncharacterized protein LOC144113010 isoform X4"
                     /calculated_mol_wt=70944
     Region          6..53
                     /region_name="DnaJ"
                     /note="DnaJ domain or J-domain. DnaJ/Hsp40 (heat shock
                     protein 40) proteins are highly conserved and play crucial
                     roles in protein translation, folding, unfolding,
                     translocation, and degradation. They act primarily by
                     stimulating the ATPase activity of Hsp70s; cl02542"
                     /db_xref="CDD:413365"
     Site            order(28..30,39,42..43,46..47)
                     /site_type="other"
                     /note="HSP70 interaction site [polypeptide binding]"
                     /db_xref="CDD:99751"
     Region          243..>436
                     /region_name="3a0801s09"
                     /note="mitochondrial precursor proteins import receptor
                     (72 kDa mitochondrial outermembrane protein)
                     (mitochondrial import receptor for the ADP/ATP carrier)
                     (translocase of outermembrane tom70); TIGR00990"
                     /db_xref="CDD:273380"
     Region          343..>433
                     /region_name="TPR"
                     /note="Tetratricopeptide (TPR) repeat [General function
                     prediction only]; COG0457"
                     /db_xref="CDD:440225"
     Region          362..392
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          397..425
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          445..474
                     /region_name="UBA_like_SF"
                     /note="UBA domain-like superfamily; cl21463"
                     /db_xref="CDD:473871"
     Region          496..562
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cd00590"
                     /db_xref="CDD:409669"
     CDS             1..643
                     /gene="LOC144113010"
                     /coded_by="XM_077645877.1:387..2318"
                     /db_xref="GeneID:144113010"
ORIGIN      
        1 mrqtanvtnd pspqdiqkry qklalkwhps khhadrrsir kfdmlcrsyi vltegrdsid
       61 kcltsremln vfikafeled lgfnldvaaq decrrplvaf adectgcrgq psahgctqcn
      121 kppsaasrcn epellatsal tpaeirellh laawasspaf tlfeeplaas tstewkkpls
      181 tehhvlpgvg tsakecvsta svgtsaapvk llvteepkqn eatvvpapsa eqlpdkktkk
      241 kasrktakrr kqrekaeakk ssakydrsss seemstedek kkvadegldp nsafvsmals
      301 rktkgansas ggvipkvskk sgkdvivrsr rlaregcdkc nvgrhkeavd lftqaiklyp
      361 qdqsyfgnrs ycysilgdyd kalkdaekai slqpqqakgy frranaqlgl qkyseaaesf
      421 rkvleidpdc heakselhsv cvfevmsmgf tleeaewsvt kgnndvqeai nllfgpdavk
      481 pdmklnpvnl egsqslwvgn ikpevtekml mslfrrfgdv hsirilhdrh cafinygnkv
      541 spgramdalq ghllcgkail irfpdhnfmd kpprsprsgg edqalaaaga tampkakppg
      601 plknnecyfw rttgcaygih cnfkhipsnr gidllldsng kci
//
LOCUS       XP_077502004             743 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113011 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502004
VERSION     XP_077502004.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645878.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..743
                     /product="uncharacterized protein LOC144113011 isoform X1"
                     /calculated_mol_wt=82760
     Region          232..>288
                     /region_name="PRP"
                     /note="Major prion protein; smart00157"
                     /db_xref="CDD:197548"
     Region          301..>363
                     /region_name="PRP"
                     /note="Major prion protein; smart00157"
                     /db_xref="CDD:197548"
     CDS             1..743
                     /gene="LOC144113011"
                     /coded_by="XM_077645878.1:57..2288"
                     /db_xref="GeneID:144113011"
ORIGIN      
        1 maalsvkall lvalaataar gqsdfsdqat kqflgmlfap fgnkpsataa asaplyeavs
       61 rplrssayqe epehlkpsys ltpprsyrrp espyrapass yprsksrsgp wisppeehwv
      121 pppshwepqp wnppkpkhga vtikirpaar eedhhhhhsh swddhghshs hgpdgwhikk
      181 kphyktvdak irippvkfrl hvspkirivs gtkdplerpp ppepeehfpw ekpstgwekp
      241 twkppsgwdk yatsgwdkph ssgwdrphss gwdkphsggw dkhhssgwdk hhtsdwdkhh
      301 ssgwdrypps gwekptrgwe rppsegwers ssggwekpaa sgwehysssg swqsppggwk
      361 pdttawdkya tswddshhkh fkphhrhypp dyprydpkfr ikvhshivtd pppstttttt
      421 eaptssslrn eppkpeerad rvdtttaltt tttelttaat tepttteptt ttteptttes
      481 tttstttvep ttfsektipp ttptttttfy svdpsqdftt pgglereapt sgptverttq
      541 spdevttnpr tyrteettma mttaettlms ttytepyeer dgmtptifpe krsrdpsttt
      601 eatteasned ypgsktsqek psnedypgsk tsqekpsned ypgsktsqek psdprfhtid
      661 deraykrdhg heattttvgk ergkvvsttp taaqttptes eehnevtmnk pessakpskv
      721 drdlpliadp sspeevsatg lna
//
LOCUS       XP_077502005             708 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113011 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502005
VERSION     XP_077502005.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645879.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..708
                     /product="uncharacterized protein LOC144113011 isoform X2"
                     /calculated_mol_wt=79213
     Region          197..>253
                     /region_name="PRP"
                     /note="Major prion protein; smart00157"
                     /db_xref="CDD:197548"
     Region          266..>328
                     /region_name="PRP"
                     /note="Major prion protein; smart00157"
                     /db_xref="CDD:197548"
     CDS             1..708
                     /gene="LOC144113011"
                     /coded_by="XM_077645879.1:173..2299"
                     /db_xref="GeneID:144113011"
ORIGIN      
        1 mlfapfgnkp sataaasapl yeavsrplrs sayqeepehl kpsysltppr syrrpespyr
       61 apassyprsk srsgpwispp eehwvpppsh wepqpwnppk pkhgavtiki rpaareedhh
      121 hhhshswddh ghshshgpdg whikkkphyk tvdakiripp vkfrlhvspk irivsgtkdp
      181 lerppppepe ehfpwekpst gwekptwkpp sgwdkyatsg wdkphssgwd rphssgwdkp
      241 hsggwdkhhs sgwdkhhtsd wdkhhssgwd ryppsgwekp trgwerppse gwersssggw
      301 ekpaasgweh ysssgswqsp pggwkpdtta wdkyatswdd shhkhfkphh rhyppdypry
      361 dpkfrikvhs hivtdpppst ttttteapts sslrneppkp eeradrvdtt taltttttel
      421 ttaatteptt teptttttep tttestttst ttvepttfse ktippttptt tttfysvdps
      481 qdfttpggle reaptsgptv erttqspdev ttnprtyrte ettmamttae ttlmsttyte
      541 pyeerdgmtp tifpekrsrd psttteatte asnedypgsk tsqekpsned ypgsktsqek
      601 psnedypgsk tsqekpsdpr fhtidderay krdhgheatt ttvgkergkv vsttptaaqt
      661 tpteseehne vtmnkpessa kpskvdrdlp liadpsspee vsatglna
//
LOCUS       XP_077502006             126 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113012 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502006
VERSION     XP_077502006.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645880.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..126
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..126
                     /product="uncharacterized protein LOC144113012 isoform X1"
                     /calculated_mol_wt=13663
     CDS             1..126
                     /gene="LOC144113012"
                     /coded_by="XM_077645880.1:352..732"
                     /db_xref="GeneID:144113012"
ORIGIN      
        1 msplsrfgav lvcacsayiv ehniinrtmd atcswfpghv kgkktgvsef kvekamyytm
       61 kgipknavfh krtaitiwst eavvlcsssg kfsggdsgrh cgytagvkll sgkcnlfdfv
      121 rkrctl
//
LOCUS       XP_077502007             126 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113012 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502007
VERSION     XP_077502007.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645881.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..126
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..126
                     /product="uncharacterized protein LOC144113012 isoform X1"
                     /calculated_mol_wt=13663
     CDS             1..126
                     /gene="LOC144113012"
                     /coded_by="XM_077645881.1:352..732"
                     /db_xref="GeneID:144113012"
ORIGIN      
        1 msplsrfgav lvcacsayiv ehniinrtmd atcswfpghv kgkktgvsef kvekamyytm
       61 kgipknavfh krtaitiwst eavvlcsssg kfsggdsgrh cgytagvkll sgkcnlfdfv
      121 rkrctl
//
LOCUS       XP_077502008             111 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113012 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502008
VERSION     XP_077502008.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645882.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..111
                     /product="uncharacterized protein LOC144113012 isoform X2"
                     /calculated_mol_wt=12176
     CDS             1..111
                     /gene="LOC144113012"
                     /coded_by="XM_077645882.1:273..608"
                     /db_xref="GeneID:144113012"
ORIGIN      
        1 mrrvfvssii nrtmdatcsw fpghvkgkkt gvsefkveka myytmkgipk navfhkrtai
       61 tiwsteavvl csssgkfsgg dsgrhcgyta gvkllsgkcn lfdfvrkrct l
//
LOCUS       XP_077502009             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113014 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502009
VERSION     XP_077502009.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645883.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144113014 isoform X1"
                     /calculated_mol_wt=14020
     CDS             1..127
                     /gene="LOC144113014"
                     /coded_by="XM_077645883.1:268..651"
                     /db_xref="GeneID:144113014"
ORIGIN      
        1 mrrvfvssii nrtmdatcsw fpghvkgkkt gvsefkveka mydtmkgipk nalfhkrtai
       61 tiwsteavvl csssgkfsgg dsgrhcgyta gvkllsgkcn lfdfvrkdah ydarglhldy
      121 tanfvyq
//
LOCUS       XP_077502010             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113014 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502010
VERSION     XP_077502010.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645884.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144113014 isoform X1"
                     /calculated_mol_wt=14020
     CDS             1..127
                     /gene="LOC144113014"
                     /coded_by="XM_077645884.1:268..651"
                     /db_xref="GeneID:144113014"
ORIGIN      
        1 mrrvfvssii nrtmdatcsw fpghvkgkkt gvsefkveka mydtmkgipk nalfhkrtai
       61 tiwsteavvl csssgkfsgg dsgrhcgyta gvkllsgkcn lfdfvrkdah ydarglhldy
      121 tanfvyq
//
LOCUS       XP_077502012             125 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113014 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502012
VERSION     XP_077502012.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645886.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..125
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..125
                     /product="uncharacterized protein LOC144113014 isoform X2"
                     /calculated_mol_wt=13605
     CDS             1..125
                     /gene="LOC144113014"
                     /coded_by="XM_077645886.1:351..728"
                     /db_xref="GeneID:144113014"
ORIGIN      
        1 mhyftrglpl psgqqkqlyy avpvassqev taegivatql esscyqgnat cstlsekmht
       61 mmlgasiltt lpilstneak dasvqiaesq qppwkartew lrscggerlg wrseswmkgv
      121 dpspq
//
LOCUS       XP_077502013             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113015 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502013
VERSION     XP_077502013.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645887.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144113015 isoform X1"
                     /calculated_mol_wt=14020
     CDS             1..127
                     /gene="LOC144113015"
                     /coded_by="XM_077645887.1:268..651"
                     /db_xref="GeneID:144113015"
ORIGIN      
        1 mrrvfvssii nrtmdatcsw fpghvkgkkt gvsefkveka mydtmkgipk nalfhkrtai
       61 tiwsteavvl csssgkfsgg dsgrhcgyta gvkllsgkcn lfdfvrkdah ydarglhldy
      121 tanfvyq
//
LOCUS       XP_077502014             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113015 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502014
VERSION     XP_077502014.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645888.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144113015 isoform X1"
                     /calculated_mol_wt=14020
     CDS             1..127
                     /gene="LOC144113015"
                     /coded_by="XM_077645888.1:268..651"
                     /db_xref="GeneID:144113015"
ORIGIN      
        1 mrrvfvssii nrtmdatcsw fpghvkgkkt gvsefkveka mydtmkgipk nalfhkrtai
       61 tiwsteavvl csssgkfsgg dsgrhcgyta gvkllsgkcn lfdfvrkdah ydarglhldy
      121 tanfvyq
//
LOCUS       XP_077502015             115 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113015 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502015
VERSION     XP_077502015.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645889.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..115
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..115
                     /product="uncharacterized protein LOC144113015 isoform X2"
                     /calculated_mol_wt=12479
     CDS             1..115
                     /gene="LOC144113015"
                     /coded_by="XM_077645889.1:351..698"
                     /db_xref="GeneID:144113015"
ORIGIN      
        1 mhyftrglpl psgqqkqlyy avpvassqev taegivatql esscyqgnat cstlsekmht
       61 mmlgasiltt lpilstneak dasvqiaesq qppwkartew lrscggerlg wrses
//
LOCUS       XP_077502016             693 aa            linear   INV 25-NOV-2025
DEFINITION  DM13 and DOMON_DOH domain-containing protein skeletor [Amblyomma
            americanum].
ACCESSION   XP_077502016
VERSION     XP_077502016.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645890.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..693
                     /product="DM13 and DOMON_DOH domain-containing protein
                     skeletor"
                     /calculated_mol_wt=76233
     Region          35..137
                     /region_name="DM13"
                     /note="Domain present in fly proteins (CG14681, CG12492,
                     CG6217), worm H06A10.1 and Arabidopsis thaliana MBG8.9;
                     smart00686"
                     /db_xref="CDD:128929"
     Region          150..253
                     /region_name="DM13"
                     /note="Electron transfer DM13; cl02735"
                     /db_xref="CDD:470661"
     Region          304..453
                     /region_name="DOMON_DOH"
                     /note="DOMON-like domain of copper-dependent
                     monooxygenases and related proteins; cd09631"
                     /db_xref="CDD:187689"
     Site            order(334,336..337,352..354,427,429,437,452)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:187689"
     CDS             1..693
                     /gene="Skel"
                     /coded_by="XM_077645890.1:38..2119"
                     /db_xref="GeneID:144113016"
ORIGIN      
        1 mlldsrawvl wtvlaaaaae pylgtrigkl nthfhgvsge lyavddttly lkdfhydgtg
       61 pdayfwagst trpdhtgfii pdekgrttpl gayhgqglvl klpagqtisk irwfavwcrk
      121 franfgdlii prnlevpkpa vlkalptwdh gvssgrvslv dsqtflvpdf kydglgpaay
      181 wwvskgpkqh rggtrllden gsdaplapat gktvfislpd glsaydfdrf gvwcdaaqvd
      241 fgsisiphdv rvppsprqla laqaeaqqra vvprvqqlqp tvapvkpasr tgsrsprgln
      301 cqvlheplgl elrwvldrdq avmqmvgrvr qgeymsfgls kddtksrmld adavvtwidr
      361 ngrghavdyy lsskeqcvgs rgscpddkis rgasnsvtml daavingyqm vtfrrpqaaq
      421 dpnydqhiys dgpqavmwav gpvndhsdas yhsahnqgdv fldfarqpaw ncpapdedat
      481 asngapeesa paegawripp vvcpadgvfq mqigpaggrk gyqavtgqvg wgiawyvngv
      541 lipeiwverg ktytfvvegg ndpentaknh plyitsdsgg gyehksvaer kaervyagvg
      601 rhkngrpypa avgrlcewql plgeqpdrys sfeqfqqrlk lqcrsgssay lrwtpdrntp
      661 dlvyyqcyth rhlgwkihvv dscaptnstg rrg
//
LOCUS       XP_077502017             784 aa            linear   INV 25-NOV-2025
DEFINITION  unextended protein-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502017
VERSION     XP_077502017.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645891.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..784
                     /product="unextended protein-like isoform X1"
                     /calculated_mol_wt=85597
     Region          247..>564
                     /region_name="TlyC"
                     /note="Hemolysin-related protein, contains CBS domains,
                     UPF0053 family [General function prediction only];
                     COG1253"
                     /db_xref="CDD:440865"
     Region          660..738
                     /region_name="cNMP"
                     /note="Cyclic nucleotide-monophosphate binding domain;
                     smart00100"
                     /db_xref="CDD:197516"
     Site            order(701..702,711..712,714)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     CDS             1..784
                     /gene="LOC144113017"
                     /coded_by="XM_077645891.1:218..2572"
                     /db_xref="GeneID:144113017"
ORIGIN      
        1 mvapstllmv lgaaaaaava atvdgvlvlg rdgrwstdep vsvgkktsll llgsglarep
       61 vsftsrdaer gstckqegam prssmdgngs ttvvlpwqgr wffclrngnn wvhqgravsl
      121 taagdpnvav vsgvrgggem vstskggyav lpvdeevlll llgthfselt ritlttersi
      181 rgvlcreqvl ietrglqplh gglgarvtvr lphaglyyvc vslagrwvhq gadplvtleg
      241 estivpfwfn lllvgtllvw sglfsgltlg lmaldktelk viescgtpee rdyarkilpl
      301 rrrgnyllcs lvlgnvcvns sftilldeml ssgpvavlls tlgivmlgei ipqaicsryg
      361 laigartili tklfmiitfp lswpisklld svlgeeignv fdreklteyl ritkdyaqle
      421 neelniifga leltkktaad imtriedvym lpydavldfe tmseivkrgy tripvydgsk
      481 qnivsllntk dltfvdpdda iplatvctfy khplsfvfed etldsllref kkghshmafv
      541 rqvvqaedrd payhvtglvt ledvieeiiq seiidetdvl mdnrrkqrrk daqllqdfsd
      601 flkigsgqqg knvvsaqlaf atyqflstgv raftsefvtp avlrrlmaqn iffslkpstg
      661 knifdagkpc dfftvilegr vrvtvgkesl vfeagpfsyf glpalergsf vpdytvtpls
      721 cvvfmkighs vymaalrasr lgrpeeppae vavqetavls ngakqtpvts telktagwsg
      781 paka
//
LOCUS       XP_077502018             872 aa            linear   INV 25-NOV-2025
DEFINITION  unextended protein-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502018
VERSION     XP_077502018.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645892.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..872
                     /product="unextended protein-like isoform X2"
                     /calculated_mol_wt=95338
     Region          335..>652
                     /region_name="TlyC"
                     /note="Hemolysin-related protein, contains CBS domains,
                     UPF0053 family [General function prediction only];
                     COG1253"
                     /db_xref="CDD:440865"
     Region          748..826
                     /region_name="cNMP"
                     /note="Cyclic nucleotide-monophosphate binding domain;
                     smart00100"
                     /db_xref="CDD:197516"
     Site            order(789..790,799..800,802)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     CDS             1..872
                     /gene="LOC144113017"
                     /coded_by="XM_077645892.1:133..2751"
                     /db_xref="GeneID:144113017"
ORIGIN      
        1 mvsvctgasg egsavsapal cfglredpqr dddedpptts rprsvvvhwr prlsvgccrr
       61 chgsrasrra rpprrrasgr rqrddqclmv apstllmvlg aaaaaavaat vdgvlvlgrd
      121 grwstdepvs vgkktsllll gsglarepvs ftsrdaergs tckqegampr ssmdgngstt
      181 vvlpwqgrwf fclrngnnwv hqgravslta agdpnvavvs gvrgggemvs tskggyavlp
      241 vdeevlllll gthfseltri tlttersirg vlcreqvlie trglqplhgg lgarvtvrlp
      301 haglyyvcvs lagrwvhqga dplvtleges tivpfwfnll lvgtllvwsg lfsgltlglm
      361 aldktelkvi escgtpeerd yarkilplrr rgnyllcslv lgnvcvnssf tilldemlss
      421 gpvavllstl givmlgeiip qaicsrygla igartilitk lfmiitfpls wpisklldsv
      481 lgeeignvfd reklteylri tkdyaqlene elniifgale ltkktaadim triedvymlp
      541 ydavldfetm seivkrgytr ipvydgskqn ivsllntkdl tfvdpddaip latvctfykh
      601 plsfvfedet ldsllrefkk ghshmafvrq vvqaedrdpa yhvtglvtle dvieeiiqse
      661 iidetdvlmd nrrkqrrkda qllqdfsdfl kigsgqqgkn vvsaqlafat yqflstgvra
      721 ftsefvtpav lrrlmaqnif fslkpstgkn ifdagkpcdf ftvilegrvr vtvgkeslvf
      781 eagpfsyfgl palergsfvp dytvtplscv vfmkighsvy maalrasrlg rpeeppaeva
      841 vqetavlsng akqtpvtste lktagwsgpa ka
//
LOCUS       XP_077502019             645 aa            linear   INV 25-NOV-2025
DEFINITION  unextended protein-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077502019
VERSION     XP_077502019.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645893.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..645
                     /product="unextended protein-like isoform X3"
                     /calculated_mol_wt=71303
     Region          108..>425
                     /region_name="TlyC"
                     /note="Hemolysin-related protein, contains CBS domains,
                     UPF0053 family [General function prediction only];
                     COG1253"
                     /db_xref="CDD:440865"
     Region          521..599
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cl00047"
                     /db_xref="CDD:469590"
     Site            order(562..563,572..573,575)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     CDS             1..645
                     /gene="LOC144113017"
                     /coded_by="XM_077645893.1:188..2125"
                     /db_xref="GeneID:144113017"
ORIGIN      
        1 mvstskggya vlpvdeevll lllgthfsel tritltters irgvlcreqv lietrglqpl
       61 hgglgarvtv rlphaglyyv cvslagrwvh qgadplvtle gestivpfwf nlllvgtllv
      121 wsglfsgltl glmaldktel kviescgtpe erdyarkilp lrrrgnyllc slvlgnvcvn
      181 ssftilldem lssgpvavll stlgivmlge iipqaicsry glaigartil itklfmiitf
      241 plswpiskll dsvlgeeign vfdrekltey lritkdyaql eneelniifg aleltkktaa
      301 dimtriedvy mlpydavldf etmseivkrg ytripvydgs kqnivsllnt kdltfvdpdd
      361 aiplatvctf ykhplsfvfe detldsllre fkkghshmaf vrqvvqaedr dpayhvtglv
      421 tledvieeii qseiidetdv lmdnrrkqrr kdaqllqdfs dflkigsgqq gknvvsaqla
      481 fatyqflstg vraftsefvt pavlrrlmaq niffslkpst gknifdagkp cdfftvileg
      541 rvrvtvgkes lvfeagpfsy fglpalergs fvpdytvtpl scvvfmkigh svymaalras
      601 rlgrpeeppa evavqetavl sngakqtpvt stelktagws gpaka
//
LOCUS       XP_077502020             507 aa            linear   INV 25-NOV-2025
DEFINITION  vesicular monoamine transporter [Amblyomma americanum].
ACCESSION   XP_077502020
VERSION     XP_077502020.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645894.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..507
                     /product="vesicular monoamine transporter"
                     /calculated_mol_wt=54697
     Region          28..456
                     /region_name="MFS_SLC18A1_2_VAT1_2"
                     /note="Vesicular amine transporters 1 (VAT1) and 2 (VAT2),
                     and similar transporters of the Major Facilitator
                     Superfamily; cd17384"
                     /db_xref="CDD:340942"
     Site            order(28..29,32..33,36,132,182..183,185..187,190,211,
                     214..215,218,286,289..290,293..295,298,326,330,383..384,
                     388,392,407,410..411,414..415,418)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340942"
     CDS             1..507
                     /gene="Vmat"
                     /coded_by="XM_077645894.1:102..1625"
                     /db_xref="GeneID:144113019"
ORIGIN      
        1 mhlldvvrgw rdsrrlvlli vavallldnm llttvvpiip nflyelhrpa dkndsaadgg
       61 palllhgttd tndwevpgrt lvpptpptpe pgttattlsp qemktrhdll snenvevgvm
      121 faskpvvqal vnpvvgpltn rvgyslpmfa gflimfvstl vfaagtnygt lffartlqgv
      181 gsactsvagm gmlaekypdd rergnamaia mgglalgvmi gppfggvmye fvsksapflv
      241 laaialldgl lqlvvlrprm rldieqgasl kalaqdpyil aaagaitfan mgiavlepsl
      301 plwlmdtmqa prwqqgavfl pasisyligt nlfgpmghkm grwlssmmgl fiiavclvci
      361 pmaknvnhli apnagigfai gmvdssmmpm lgylvdirha svygsvyaig dtafcvgfvl
      421 gpiisssvvk tlgfrallym tatvcfvysp vmfvlknppg rqenqslllr sgssdiryms
      481 yaneddspeq qqqqqqqqqq qqqqqqr
//
LOCUS       XP_077502021            1466 aa            linear   INV 25-NOV-2025
DEFINITION  eukaryotic translation initiation factor 2 alpha kinase Gcn2
            [Amblyomma americanum].
ACCESSION   XP_077502021
VERSION     XP_077502021.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645895.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1466
                     /product="eukaryotic translation initiation factor 2 alpha
                     kinase Gcn2"
                     /calculated_mol_wt=164684
     Region          7..126
                     /region_name="RWD_GCN2"
                     /note="RWD domain of eIF-2-alpha kinase GCN2 and related
                     proteins; cd23823"
                     /db_xref="CDD:467659"
     Region          233..448
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(233..235,238,249,280,298..301,344,348..349,351,
                     361..362)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     Region          479..835
                     /region_name="STKc_EIF2AK4_GCN2_rpt2"
                     /note="Catalytic domain, repeat 2, of the Serine/Threonine
                     kinase, eukaryotic translation Initiation Factor 2-Alpha
                     Kinase 4 or General Control Non-derepressible-2; cd14046"
                     /db_xref="CDD:270948"
     Site            order(479..480,483..484,530..531,534..535,537..538,540,
                     546..553,634,636..637,677,680..682)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:270948"
     Site            order(491..492,497,499,512,514,641..644,648,691..692,694,
                     705,740..742,777,780..787)
                     /site_type="active"
                     /db_xref="CDD:270948"
     Site            order(491..492,499,512,514,545,641..644,687,689,692,694,
                     705)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270948"
     Site            order(704..713,721..725,731..742)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270948"
     Site            order(740..742,777,780..787)
                     /site_type="other"
                     /note="eIF2alpha (substrate) binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270948"
     Region          <911..1322
                     /region_name="PLN02972"
                     /note="Histidyl-tRNA synthetase"
                     /db_xref="CDD:215525"
     CDS             1..1466
                     /gene="Gcn2"
                     /coded_by="XM_077645895.1:1..4401"
                     /db_xref="GeneID:144113020"
ORIGIN      
        1 meeedlkerq enemkvlqsm fpndvkdlrk qdkwktwrpp ellltlrpqq smtpfqahvq
       61 vdlhvrcssq ypndvpyvdv cnqkglskqa lvelrnelle ltkrlvgevm vlelaqhvqw
      121 flrqhntper gsfydemlkn rekqeaekar eqqqqlnqqr leeekqrqaf eleimrhqqe
      181 mkvearrrrt dskiaegker nccarkpell nfssktgera iqkcsclghs erghstfggf
      241 dsatgelvci vqwtlktayh vatveqelqt lmklkhtnlv hylgslyvpe krafyilqef
      301 vkgcplssfi aqgipldval lrnyttgilq alqflhsnsv vhkdlressi fvdcsgvvkv
      361 adfsipkkiv dlclppsyds gptskskkkn dilqlgkvll sllrgehaas tdipsnlsld
      421 ardflercla aqeqdrwsce qllshpflve rgpsledepl ddegpdesgt cplssaghsr
      481 lqgefevlrw lgrggfgdvf kvrnkldrcv yaikriqlnp sskvlnrkil revkllsrln
      541 henvvryyns wievttqeqv petlassgvs tespkgtslg wslpdvnadd ssssdnmfgc
      601 afgessssdn vvfegsgkee sevseepaav aharqfmfiq mefcekstlr tvidsglhre
      661 psrmwrlfre vieglahihq qgmihrdlkp vnifldsgdh vkigdfglat tallsrvets
      721 eapehidqpt sgsltgrvgt tlytapelfv stggrvvysq kvdiyslgii lfemcwpals
      781 tamervklla nlrlpsitlp teacdllssq qvslirwllq hdpsqrpsaa ellaspqlpp
      841 phlqeaqvte vlnhtvsnpq skaykhlvna lfqqeptvvq dyaydvdmrk gnplpqsall
      901 frhvkmsler vlvrhggmpv viptmlpkcp gsedddnkvh fmdhggliva lphnmrvpfa
      961 ryiarrsdav slrryaiekv yrsrkvfgch prelhecafd iicsnhqsal thcaellhig
     1021 sevvaefpql qargysirig htgllqslll hcgvsenkws qvltslrtvq ssskaqlqqg
     1081 ldaanlgdqa valltqfyev ednfakvssm yrfvvrqkgp aaalarqalh dleavlqsss
     1141 alafqlpvti vpclvcderm ysgivfeiac qshrktrhqg rdllavggry dklvasfava
     1201 gakrdlaaag isfsvekivq alaedgetps lvecvlgnig dmsiamreqq lallvrlwnm
     1261 dvpavvapqd gieeaayfck enfvphlall kelepgylrl rslekdrfte krlsvselce
     1321 ffdkapqaes pkpefnssgp tvrivfsvve klstsnkrry esqiatqlap laqghlgrvp
     1381 vldviavelt gdvlrscval lnmeadgrsy essvtvlvek hprykkqlll ltekvysfif
     1441 eikrtvfvly alqdnnykvv ilpsrt
//
LOCUS       XP_077502022             871 aa            linear   INV 25-NOV-2025
DEFINITION  unextended protein-like [Amblyomma americanum].
ACCESSION   XP_077502022
VERSION     XP_077502022.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645896.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (GKSP01013297.1) annotated
            using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 524 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..871
                     /product="unextended protein-like"
                     /calculated_mol_wt=96391
     Region          341..>647
                     /region_name="TlyC"
                     /note="Hemolysin-related protein, contains CBS domains,
                     UPF0053 family [General function prediction only];
                     COG1253"
                     /db_xref="CDD:440865"
     Region          <733..>789
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cl00047"
                     /db_xref="CDD:469590"
     Site            order(772..773,797..798,810)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     CDS             1..871
                     /gene="LOC144113021"
                     /coded_by="XM_077645896.1:670..3285"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 524 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144113021"
ORIGIN      
        1 masarnglhr flvlwlcsrc salyigqpla nredfvtvdk ieavctewyp dsarvprvfp
       61 nsrvavrlrg tgllpnrtki aftsesleng qechsqlfva rqlrvradsn gdvvvqgvip
      121 aatsqdllyl ctrnrgrwrh qgravrlmya ttptrhagpl rmrrnlgpgr rstdrnvtgp
      181 krnqskssld ggetaqvlgl rafgervatl dsgmtqvpag tavelqlfgn gftnqssfaf
      241 theaaprgsa cdevptvava hatkvgggka svlvslpgas iryyvcikap fsedvkwvhq
      301 gdepwvtlvt qgrlmpvwvq aiilaallll sglfsglnlg lmaldktelr viescgtpse
      361 rkwakviapl rnhgnyllcs lllgnvlvns tltillddlt sgvvavvgst isivifgeii
      421 pqaicsrhgl qigartlfit kvfmaltlpl swpiskildw vlgeeighvy dreklieyir
      481 ltkdynmlet eevdiisgal elkkktanea mtrmddvfml pvtavldfet vsqiigqgyt
      541 ripvfegdrn nvvgllnikd lafvdpedei plrtlcefyn hpltfvfede tlvnllnefk
      601 kgqshmafvr rvntegdgdp fyellglvtl edvieeilqs eiidetdvlm dnrrklkrke
      661 aqvrqdfsdf aklgygrqgk hiiapqlala tfqylatsve pfkknlises vlrrlmlqni
      721 fisikmhsrd dpviyvagqp adyfvlvleg rvsvtvgrek llfetgpfst fgipaiyryt
      781 etsegaaaqn laagepsswp vpeepfvpdy svqavadtvy mrvhrsvyla alkasrldrp
      841 atrtdlevdh mllssapeqp gdrspllgds s
//
LOCUS       XP_077502023             406 aa            linear   INV 25-NOV-2025
DEFINITION  heme A synthase COX15 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502023
VERSION     XP_077502023.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645897.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..406
                     /product="heme A synthase COX15 isoform X1"
                     /calculated_mol_wt=45304
     Region          39..399
                     /region_name="COX15-CtaA"
                     /note="Cytochrome oxidase assembly protein; cl19388"
                     /db_xref="CDD:473166"
     CDS             1..406
                     /gene="LOC144113022"
                     /coded_by="XM_077645897.1:321..1541"
                     /db_xref="GeneID:144113022"
ORIGIN      
        1 mnvsqrclfs rasnsylrai ifrqevcrhs lvhkqcrhtl qfqtigrrfq vtavpsateq
       61 ltgksqravg rwllacsgma fgsivlggvt rltksglsmv hwhpfaefpp sgqhqweqef
      121 lkyqeypeyk asdremtlae fkriwyieyi hrmwgrtiga vfytgaawfw wrgclsrrak
      181 vhvgllavgl afqgflgwfm vksglqdqph vsqyrlaahl gtalawysla fwsglshlaa
      241 rpgsttalls aamyrgvhgv lglvfvtams gaivaglrag lvynsfpkma drwvpsdima
      301 lepklsnfte npttaqfdhr ilgesvvviv tglwlwgrkq plpprakkal hcllaaawlq
      361 atlgvstllt yvpvslassh qagavtllsv alwlahelkl lrripk
//
LOCUS       XP_077502024             328 aa            linear   INV 25-NOV-2025
DEFINITION  heme A synthase COX15 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502024
VERSION     XP_077502024.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645898.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..328
                     /product="heme A synthase COX15 isoform X2"
                     /calculated_mol_wt=36401
     Region          1..321
                     /region_name="COX15-CtaA"
                     /note="Cytochrome oxidase assembly protein; cl19388"
                     /db_xref="CDD:473166"
     CDS             1..328
                     /gene="LOC144113022"
                     /coded_by="XM_077645898.1:319..1305"
                     /db_xref="GeneID:144113022"
ORIGIN      
        1 mafgsivlgg vtrltksgls mvhwhpfaef ppsgqhqweq eflkyqeype ykasdremtl
       61 aefkriwyie yihrmwgrti gavfytgaaw fwwrgclsrr akvhvgllav glafqgflgw
      121 fmvksglqdq phvsqyrlaa hlgtalawys lafwsglshl aarpgsttal lsaamyrgvh
      181 gvlglvfvta msgaivaglr aglvynsfpk madrwvpsdi malepklsnf tenpttaqfd
      241 hrilgesvvv ivtglwlwgr kqplpprakk alhcllaaaw lqatlgvstl ltyvpvslas
      301 shqagavtll svalwlahel kllrripk
//
LOCUS       XP_077502025             267 aa            linear   INV 25-NOV-2025
DEFINITION  bridging integrator 3-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502025
VERSION     XP_077502025.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645899.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..267
                     /product="bridging integrator 3-like isoform X1"
                     /calculated_mol_wt=29803
     Region          34..259
                     /region_name="BAR"
                     /note="The Bin/Amphiphysin/Rvs (BAR) domain, a
                     dimerization module that binds membranes and detects
                     membrane curvature; cl12013"
                     /db_xref="CDD:472257"
     Site            order(52,55..56,60,62..63,66..67,69..70,73..74,76..77,
                     80..81,83..84,87,107,110..111,206,209..210,212,217,
                     220..221,223..224,227..228,231..232,234,236,239..240,
                     242..243)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153271"
     CDS             1..267
                     /gene="LOC144113023"
                     /coded_by="XM_077645899.1:79..882"
                     /db_xref="GeneID:144113023"
ORIGIN      
        1 mqcsfmavse trrdggasfp srnplkrlsk tssrtspgdd ldektieehv skfvrlerdt
       61 kklhndakky edtmlalyhc ekkmssdlas gelcnespel rrlveewlgf assmdtavdd
      121 hlvavrrclv dplkryqsvf aevqaalkrr dqavqeclrl eqrverlggr estgpnlarl
      181 secrqaleta haelatmeal laqdlprwya asslylqpcl ealvhsqtlh wgqattrahd
      241 lmapgarspv elrlaaaral sivslpt
//
LOCUS       XP_077502026             248 aa            linear   INV 25-NOV-2025
DEFINITION  bridging integrator 3-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502026
VERSION     XP_077502026.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645900.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..248
                     /product="bridging integrator 3-like isoform X2"
                     /calculated_mol_wt=27805
     Region          15..240
                     /region_name="BAR"
                     /note="The Bin/Amphiphysin/Rvs (BAR) domain, a
                     dimerization module that binds membranes and detects
                     membrane curvature; cl12013"
                     /db_xref="CDD:472257"
     Site            order(33,36..37,41,43..44,47..48,50..51,54..55,57..58,
                     61..62,64..65,68,88,91..92,187,190..191,193,198,201..202,
                     204..205,208..209,212..213,215,217,220..221,223..224)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153274"
     CDS             1..248
                     /gene="LOC144113023"
                     /coded_by="XM_077645900.1:172..918"
                     /db_xref="GeneID:144113023"
ORIGIN      
        1 mswnplkrls ktssrtspgd dldektieeh vskfvrlerd tkklhndakk yedtmlalyh
       61 cekkmssdla sgelcnespe lrrlveewlg fassmdtavd dhlvavrrcl vdplkryqsv
      121 faevqaalkr rdqavqeclr leqrverlgg restgpnlar lsecrqalet ahaelatmea
      181 llaqdlprwy aasslylqpc lealvhsqtl hwgqattrah dlmapgarsp velrlaaara
      241 lsivslpt
//
LOCUS       XP_077502027             527 aa            linear   INV 25-NOV-2025
DEFINITION  clathrin interactor lqfR [Amblyomma americanum].
ACCESSION   XP_077502027
VERSION     XP_077502027.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645901.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..527
                     /product="clathrin interactor lqfR"
                     /calculated_mol_wt=56420
     Region          24..149
                     /region_name="ENTH_EpsinR"
                     /note="Epsin N-Terminal Homology (ENTH) domain of
                     Epsin-related protein; cd16989"
                     /db_xref="CDD:340786"
     Site            order(29,33..34,67,74,78)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:340786"
     Site            order(52..54,94..96,142..143,146,149)
                     /site_type="other"
                     /note="SNARE interaction site [polypeptide binding]"
                     /db_xref="CDD:340786"
     Region          <294..>415
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     CDS             1..527
                     /gene="lqfR"
                     /coded_by="XM_077645901.1:346..1929"
                     /db_xref="GeneID:144113024"
ORIGIN      
        1 mnmpwkikel vdkatnmvmn ytemeakvre atndvawgpp gqlmqeiaqa tfsydhfpev
       61 mgmlwkrilq dnkrcyrrpy kglllldylv rngservvts arehiydlrs lenysfidei
      121 gkdqgvnvrq kvkdlidfiq dderlreerk kakkakdkyi glsgeslgfr ysdrnkfddm
      181 dhgprlgrrh sfedspdnsn deerderpgg gvtyrdeprt pswkpqakkl dmgaaanygr
      241 easppaspaa ttaaaplpvp svpaadllgd iastptangd fadfsqfqsa taappsaqde
      301 ftdfasftpt ssiptglidl pvqatsvqps sltspaappv lssvqpslht spisqpvlqa
      361 gplaglqpvi panplagmqq tspltplqpp lqtsplvtgl qppsggmmgl pmgyplaaqp
      421 atfhmgyhlp pqaglaqpva fqglpglvrq sspgctpltp spasaapsal ggpmggqapp
      481 gkcgktwvds gsinisvdnl sltskyakpa apsmnqlaag nslgrlq
//
LOCUS       XP_077502028             455 aa            linear   INV 25-NOV-2025
DEFINITION  protein phosphatase 2 regulatory subunit tws isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502028
VERSION     XP_077502028.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645902.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..455
                     /product="protein phosphatase 2 regulatory subunit tws
                     isoform X1"
                     /calculated_mol_wt=52435
     Region          26..451
                     /region_name="CDC55"
                     /note="Serine/threonine protein phosphatase 2A, regulatory
                     subunit [Signal transduction mechanisms]; COG5170"
                     /db_xref="CDD:227498"
     Region          46..94
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          112..183
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          189..225
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          239..290
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          297..333
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          354..395
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          428..451
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..455
                     /gene="tws"
                     /coded_by="XM_077645902.1:275..1642"
                     /db_xref="GeneID:144113025"
ORIGIN      
        1 mvsadlssqk pkkraeydge sngeiqwcfs qvkgtldddv teadiiscve fnhdgdllat
       61 gdkggrvvif qrdpasrqsq prrgeynvys tfqshepefd ylksleieek inkirwlrrk
      121 nqahfllstn dktiklwkvs erdkridgys sraetggmts lrvptlqpmd llveatprri
      181 fanahtyhin sisvnsdqet ylsaddlrin lwhleitdqs fnivdikptn meeltevita
      241 aefhpsqchm fvyssskgti rlcdmraaal cdqhaklfee pedptnrsff seiissisdv
      301 kfsnngrymi srdyltvkvw dlnmdnkpve cynvheylrt klcslyendc ifdkfeccwn
      361 gtdsfimtgs ynnffrvfdr takrdvtlea sreiakprtv lkarkvctgg krkkeeisvd
      421 cldfnrkilh tawhpqenvv avaatnnlyi fqekf
//
LOCUS       XP_077502029             437 aa            linear   INV 25-NOV-2025
DEFINITION  protein phosphatase 2 regulatory subunit tws isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502029
VERSION     XP_077502029.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645903.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..437
                     /product="protein phosphatase 2 regulatory subunit tws
                     isoform X2"
                     /calculated_mol_wt=50386
     Region          1..433
                     /region_name="CDC55"
                     /note="Serine/threonine protein phosphatase 2A, regulatory
                     subunit [Signal transduction mechanisms]; COG5170"
                     /db_xref="CDD:227498"
     Region          28..76
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          94..165
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          171..207
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          221..272
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          279..315
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          336..377
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          410..433
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..437
                     /gene="tws"
                     /coded_by="XM_077645903.1:188..1501"
                     /db_xref="GeneID:144113025"
ORIGIN      
        1 magngeiqwc fsqvkgtldd dvteadiisc vefnhdgdll atgdkggrvv ifqrdpasrq
       61 sqprrgeynv ystfqshepe fdylksleie ekinkirwlr rknqahflls tndktiklwk
      121 vserdkridg yssraetggm tslrvptlqp mdllveatpr rifanahtyh insisvnsdq
      181 etylsaddlr inlwhleitd qsfnivdikp tnmeeltevi taaefhpsqc hmfvyssskg
      241 tirlcdmraa alcdqhaklf eepedptnrs ffseiissis dvkfsnngry misrdyltvk
      301 vwdlnmdnkp vecynvheyl rtklcslyen dcifdkfecc wngtdsfimt gsynnffrvf
      361 drtakrdvtl easreiakpr tvlkarkvct ggkrkkeeis vdcldfnrki lhtawhpqen
      421 vvavaatnnl yifqekf
//
LOCUS       XP_077502030             420 aa            linear   INV 25-NOV-2025
DEFINITION  protein phosphatase 2 regulatory subunit tws isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502030
VERSION     XP_077502030.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645904.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..420
                     /product="protein phosphatase 2 regulatory subunit tws
                     isoform X3"
                     /calculated_mol_wt=48600
     Region          43..416
                     /region_name="CDC55"
                     /note="Serine/threonine protein phosphatase 2A, regulatory
                     subunit [Signal transduction mechanisms]; COG5170"
                     /db_xref="CDD:227498"
     Region          154..190
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          204..255
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          262..298
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          319..360
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          393..416
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..420
                     /gene="tws"
                     /coded_by="XM_077645904.1:293..1555"
                     /db_xref="GeneID:144113025"
ORIGIN      
        1 mtvtcwrrat kvvglsssse tprasmpsar ttktewgpsq srqsqprrge ynvystfqsh
       61 epefdylksl eieekinkir wlrrknqahf llstndktik lwkvserdkr idgyssraet
      121 ggmtslrvpt lqpmdllvea tprrifanah tyhinsisvn sdqetylsad dlrinlwhle
      181 itdqsfnivd ikptnmeelt evitaaefhp sqchmfvyss skgtirlcdm raaalcdqha
      241 klfeepedpt nrsffseiis sisdvkfsnn grymisrdyl tvkvwdlnmd nkpvecynvh
      301 eylrtklcsl yendcifdkf eccwngtdsf imtgsynnff rvfdrtakrd vtleasreia
      361 kprtvlkark vctggkrkke eisvdcldfn rkilhtawhp qenvvavaat nnlyifqekf
//
LOCUS       XP_077502031             420 aa            linear   INV 25-NOV-2025
DEFINITION  protein phosphatase 2 regulatory subunit tws isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502031
VERSION     XP_077502031.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645905.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..420
                     /product="protein phosphatase 2 regulatory subunit tws
                     isoform X3"
                     /calculated_mol_wt=48600
     Region          43..416
                     /region_name="CDC55"
                     /note="Serine/threonine protein phosphatase 2A, regulatory
                     subunit [Signal transduction mechanisms]; COG5170"
                     /db_xref="CDD:227498"
     Region          154..190
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          204..255
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          262..298
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          319..360
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          393..416
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..420
                     /gene="tws"
                     /coded_by="XM_077645905.1:247..1509"
                     /db_xref="GeneID:144113025"
ORIGIN      
        1 mtvtcwrrat kvvglsssse tprasmpsar ttktewgpsq srqsqprrge ynvystfqsh
       61 epefdylksl eieekinkir wlrrknqahf llstndktik lwkvserdkr idgyssraet
      121 ggmtslrvpt lqpmdllvea tprrifanah tyhinsisvn sdqetylsad dlrinlwhle
      181 itdqsfnivd ikptnmeelt evitaaefhp sqchmfvyss skgtirlcdm raaalcdqha
      241 klfeepedpt nrsffseiis sisdvkfsnn grymisrdyl tvkvwdlnmd nkpvecynvh
      301 eylrtklcsl yendcifdkf eccwngtdsf imtgsynnff rvfdrtakrd vtleasreia
      361 kprtvlkark vctggkrkke eisvdcldfn rkilhtawhp qenvvavaat nnlyifqekf
//
LOCUS       XP_077502032              60 aa            linear   INV 25-NOV-2025
DEFINITION  BBSome-interacting protein 1-like [Amblyomma americanum].
ACCESSION   XP_077502032
VERSION     XP_077502032.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645906.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..60
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..60
                     /product="BBSome-interacting protein 1-like"
                     /calculated_mol_wt=6598
     Region          3..54
                     /region_name="BBIP10"
                     /note="Cilia BBSome complex subunit 10; pfam14777"
                     /db_xref="CDD:464310"
     CDS             1..60
                     /gene="LOC144113027"
                     /coded_by="XM_077645906.1:143..325"
                     /db_xref="GeneID:144113027"
ORIGIN      
        1 mfgaavhevv psrgllcres cvrpvlckpk llplqsisle klerlqaqqk tqhqpspksh
//
LOCUS       XP_077502033             146 aa            linear   INV 25-NOV-2025
DEFINITION  zinc transporter ZIP13-like [Amblyomma americanum].
ACCESSION   XP_077502033
VERSION     XP_077502033.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645907.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..146
                     /product="zinc transporter ZIP13-like"
                     /calculated_mol_wt=15369
     Region          69..>142
                     /region_name="Zip"
                     /note="ZIP Zinc transporter; cl00437"
                     /db_xref="CDD:444905"
     CDS             1..146
                     /gene="LOC144113028"
                     /coded_by="XM_077645907.1:3808..4248"
                     /db_xref="GeneID:144113028"
ORIGIN      
        1 mplaaqllrl gvvaasgldg vlcdhgrsvn ttvtgglpll rvwqlwgpas epgpfvtaas
       61 dvvrtyqtwl fsiaaacvvg lsgvfplllv pvdtgvnlkq nrsgcrtlkl llsfavggll
      121 gdvflhllpe awgrlhrgvc shrsiy
//
LOCUS       XP_077502034             146 aa            linear   INV 25-NOV-2025
DEFINITION  zinc transporter ZIP13-like [Amblyomma americanum].
ACCESSION   XP_077502034
VERSION     XP_077502034.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645908.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..146
                     /product="zinc transporter ZIP13-like"
                     /calculated_mol_wt=15369
     Region          69..>142
                     /region_name="Zip"
                     /note="ZIP Zinc transporter; cl00437"
                     /db_xref="CDD:444905"
     CDS             1..146
                     /gene="LOC144113028"
                     /coded_by="XM_077645908.1:2986..3426"
                     /db_xref="GeneID:144113028"
ORIGIN      
        1 mplaaqllrl gvvaasgldg vlcdhgrsvn ttvtgglpll rvwqlwgpas epgpfvtaas
       61 dvvrtyqtwl fsiaaacvvg lsgvfplllv pvdtgvnlkq nrsgcrtlkl llsfavggll
      121 gdvflhllpe awgrlhrgvc shrsiy
//
LOCUS       XP_077502035             550 aa            linear   INV 25-NOV-2025
DEFINITION  hexosaminidase D-like [Amblyomma americanum].
ACCESSION   XP_077502035
VERSION     XP_077502035.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645909.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..550
                     /product="hexosaminidase D-like"
                     /calculated_mol_wt=62301
     Region          79..388
                     /region_name="GH20_GcnA-like"
                     /note="Glycosyl hydrolase family 20 (GH20) catalytic
                     domain of N-acetyl-beta-D-glucosaminidase (GcnA, also
                     known as BhsA) and related proteins. GcnA is an
                     exoglucosidase which cleaves N-acetyl-beta-D-galactosamine
                     (NAG) and N-acetyl-beta-D-galactosamine...; cd06565"
                     /db_xref="CDD:119335"
     Site            order(87,161,217..218,264,290,364,366)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:119335"
     CDS             1..550
                     /gene="LOC144113029"
                     /coded_by="XM_077645909.1:269..1921"
                     /db_xref="GeneID:144113029"
ORIGIN      
        1 mspramapmk irllkliaav vviafvilyw qstrtvertp vetvqwvadv ltdppstasa
       61 phasfqqslr vppvqfderi vhfdlkgapp kleyydavfp llrklgatgl lmeyedmfpy
      121 tgvlsplaas naykeaeiah ilatarsnql qviplvqtfg hlefalklpe fadlrelshs
      181 pqalcptlna slslvkamld qvlhlhpsaq yvhigcdevy slgqcarcer rlgaanitkd
      241 klfldhvekv aahvaqygvr pimwddmlrg ieadevqrwg athpvdlmvw qytpniskhl
      301 edqhwlkyar fqglwiasaf kgatgpkqll pdtkyhlrnh hswletlnnh rellnirgit
      361 ltgwqrydhf atlcellpas lpslaanlvy lqhgcldepq lsqikellqc nrqlplfdda
      421 gssllfcqfp gakvltgvqq lvtlynqkkv meknshyvgw ldsyqvrlsf vspdhiveat
      481 knltellsts nhlhkylgda lnqlydvptv iewigtfldp lhaeltlleq qvqrllnysn
      541 wprrplerat
//
LOCUS       XP_077502036             550 aa            linear   INV 25-NOV-2025
DEFINITION  hexosaminidase D-like [Amblyomma americanum].
ACCESSION   XP_077502036
VERSION     XP_077502036.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645910.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..550
                     /product="hexosaminidase D-like"
                     /calculated_mol_wt=62301
     Region          79..388
                     /region_name="GH20_GcnA-like"
                     /note="Glycosyl hydrolase family 20 (GH20) catalytic
                     domain of N-acetyl-beta-D-glucosaminidase (GcnA, also
                     known as BhsA) and related proteins. GcnA is an
                     exoglucosidase which cleaves N-acetyl-beta-D-galactosamine
                     (NAG) and N-acetyl-beta-D-galactosamine...; cd06565"
                     /db_xref="CDD:119335"
     Site            order(87,161,217..218,264,290,364,366)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:119335"
     CDS             1..550
                     /gene="LOC144113029"
                     /coded_by="XM_077645910.1:159..1811"
                     /db_xref="GeneID:144113029"
ORIGIN      
        1 mspramapmk irllkliaav vviafvilyw qstrtvertp vetvqwvadv ltdppstasa
       61 phasfqqslr vppvqfderi vhfdlkgapp kleyydavfp llrklgatgl lmeyedmfpy
      121 tgvlsplaas naykeaeiah ilatarsnql qviplvqtfg hlefalklpe fadlrelshs
      181 pqalcptlna slslvkamld qvlhlhpsaq yvhigcdevy slgqcarcer rlgaanitkd
      241 klfldhvekv aahvaqygvr pimwddmlrg ieadevqrwg athpvdlmvw qytpniskhl
      301 edqhwlkyar fqglwiasaf kgatgpkqll pdtkyhlrnh hswletlnnh rellnirgit
      361 ltgwqrydhf atlcellpas lpslaanlvy lqhgcldepq lsqikellqc nrqlplfdda
      421 gssllfcqfp gakvltgvqq lvtlynqkkv meknshyvgw ldsyqvrlsf vspdhiveat
      481 knltellsts nhlhkylgda lnqlydvptv iewigtfldp lhaeltlleq qvqrllnysn
      541 wprrplerat
//
LOCUS       XP_077502037            1527 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113030 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502037
VERSION     XP_077502037.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645911.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1527
                     /product="uncharacterized protein LOC144113030 isoform X1"
                     /calculated_mol_wt=163483
     Region          6..97
                     /region_name="FHA"
                     /note="forkhead associated (FHA) domain superfamily;
                     cl00062"
                     /db_xref="CDD:469597"
     Site            order(30..31,45,46,66..67)
                     /site_type="other"
                     /note="phosphopeptide binding site [polypeptide binding]"
                     /db_xref="CDD:438714"
     CDS             1..1527
                     /gene="LOC144113030"
                     /coded_by="XM_077645911.1:124..4707"
                     /db_xref="GeneID:144113030"
ORIGIN      
        1 mpwyprifti kkdgtrgskh rfqrgrcsvg taagcdihir gadssyclfl adknmlaqvv
       61 nvtgsagvkh ngervrntsf lshgdivqig srqfrfewvn krkkksaqkr tkksskvaaa
      121 shqpkaagda resstknhes dnearheqqs rnralhssts stplidesrk rarrsrsclp
      181 gmhnsfeeey psfqegqscs slkrrsqsle nsiaersckl rrwrrnkglk vnsfshkens
      241 sgfltpgsqs wypngkattd esfldspgls rralirsqas lchtslsssa gslgcesffk
      301 tpnhtdsisk rvlgpltaqd vsnsslgnkr aykrlshian prshqshaks ftasplssrs
      361 rpslekntng ahtpsdhsar stwansslgq nsectnsfkr vvtadetiss fdgsncegqf
      421 gastlgfleq eegvsakktg skcaistdst skkrrrrrkk kwnstsfnes pgdkegaaev
      481 psraarssit nsavdsltwv qlgsegllqg vfyvpavfln mvtpasnnrs kkespeqeha
      541 vaseegddkk egvppqyard esqsdqesle hmgimaespk vtvskqtfqc ssqtlensre
      601 salekatllt rsgrfshtpt skemreevse leatgtasal kaptsrrtlr ssnvtkemnr
      661 avstlspghm tlddvdvenk eaqaqqnvdt pkpfqtsggk qtpasskisa seeateqvim
      721 gtspierppl mnispvvaes vgtqstrspc altlknsste atgdetvglr pnslnasgqk
      781 kavsrkvnie apqqemadtp kslkasaakq isaskkikeg qgdlkqttqa spsecvsfdn
      841 asaategfvr ftisslssas rnvnavtpgm eevcsqnvmg gsrvsskske alaqetvdtp
      901 kslntsggkq tpasskisas eeateqvimg tspierpplm nispvvaesv gtqstrspca
      961 ltlknsstea tgdetvglrp nslnasgqkk avsrkvniea pqqemadtpk slkasaakqi
     1021 saskkikegq gdlkqttqas psecvsfdna saategfvrf tisslssasr nvnavtpgme
     1081 evcsqnvmgg srvsskskea laqekvdtpk slktsggkqt passkisase eateqvimgt
     1141 spierpplmn ispvvaesvg tqstrspcal tlknssteat gdetvglrpn slnasgekkv
     1201 vsrkvnieap qqemadtpks lkaseakqis askkikegqg dhkqttqasp secvsfdnas
     1261 aategfvrft isslssasrn vnavtpgmee vcsqnvmggs rvsskskeal aqetvdtpks
     1321 lntsggkqtp asgeiivlvk eateqnivga spvespksle astahwassa snvvkedleq
     1381 etraspskqv neveevpele tagvslskcs satnvhalad epvrptrgcc trgsrkdnee
     1441 areekkvdhp qstkatrakh tqgsrkavkv dmaqtateat prktrgrrak aletalewad
     1501 dkpdasrrta edhseeappa krarkkv
//
LOCUS       XP_077502038             156 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113030 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502038
VERSION     XP_077502038.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645912.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..156
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..156
                     /product="uncharacterized protein LOC144113030 isoform X2"
                     /calculated_mol_wt=17403
     Region          6..97
                     /region_name="FHA"
                     /note="forkhead associated (FHA) domain superfamily;
                     cl00062"
                     /db_xref="CDD:469597"
     Site            order(30..31,45,46,66..67)
                     /site_type="other"
                     /note="phosphopeptide binding site [polypeptide binding]"
                     /db_xref="CDD:438714"
     CDS             1..156
                     /gene="LOC144113030"
                     /coded_by="XM_077645912.1:124..594"
                     /db_xref="GeneID:144113030"
ORIGIN      
        1 mpwyprifti kkdgtrgskh rfqrgrcsvg taagcdihir gadssyclfl adknmlaqvv
       61 nvtgsagvkh ngervrntsf lshgdivqig srqfrfewvn krkkksaqkr tkksskvaaa
      121 shqpkewadd kpdasrrtae dhseeappak rarkkv
//
LOCUS       XP_077502039            1109 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113031 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502039
VERSION     XP_077502039.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645913.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1109
                     /product="uncharacterized protein LOC144113031 isoform X1"
                     /calculated_mol_wt=120216
     Region          6..97
                     /region_name="FHA"
                     /note="forkhead associated (FHA) domain superfamily;
                     cl00062"
                     /db_xref="CDD:469597"
     Site            order(30..31,45,46,66..67)
                     /site_type="other"
                     /note="phosphopeptide binding site [polypeptide binding]"
                     /db_xref="CDD:438714"
     CDS             1..1109
                     /gene="LOC144113031"
                     /coded_by="XM_077645913.1:195..3524"
                     /db_xref="GeneID:144113031"
ORIGIN      
        1 mpwyprifti kkdgtrgskh rfqrgrcsvg taagcdihir gadssyclfl vdknmlaqvv
       61 nvtgsagvkh ngervrntsf lshgdivqig srqfrfewvn krkkksaqkr tkksskvaaa
      121 shqpkaagda resstkhhes dnearhkqqs rnralhssts stplidesrk rarrsrsclp
      181 gmhnsfeeey psfqegqscs slkrrsqsle nsiaersckl rrwrrkkglk vnsfshkens
      241 sgfltpgsqs wypngkattd esfldspgls rralirsqts lchtslsssa gslgcesffk
      301 tpnhtdsisk relgpltaqd vsnsslgnkr aykrlshian prshqshaks ftasplssrs
      361 rpslekntng ahtpsdhsar stwansslgq nsectnsfkr vvtadetiss fdgsncegqf
      421 gastlgfleq eegvsakktg skcaistdst skkrrrrrkk kwnstsfnes pgdkegaaev
      481 psraarssit nsavdsltwv qlgsegllqg vfyvpavfln mvtpasnnrs kkespeqeha
      541 vaseegddkk egvppqyard esqsdqesle hmgimaespk vtvskqtfqc ssqtlensre
      601 salekatllt rsgrfshtpt skemreevse leatgtasal kaptsrqtlp srnvteemnr
      661 avstlspghm tlddvdvenk eaqaqknvdt pkpfqtsggk qtpasskisa seeateqvim
      721 gtspierppl mnispvvaes vgtqstrspc altlknsste atgdetvglr pnslnasgek
      781 kvvsrkvnie apqqemadtp kslkaseakq isaskkikeg qgdlkqttqa spsecvsfdn
      841 asaategfvr ftisslssas rnvsavtpgm eevcsqnvmg gsrvsskske alaqetvdtp
      901 kslntsggkq tpasgeiivl vkeateqniv gaspvespks leastahwap sasnvvkedl
      961 eqetraspsk qvneveevpe letagvslsk cssatnvhal adepvrptrg cctrgsrkdn
     1021 eeareekkvd hpqstkatra khtqgsrkav kvdmaqtate atprktrgrr akaletalew
     1081 addkpdasrr taedhseeap pakrarkkv
//
LOCUS       XP_077502040             156 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113031 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502040
VERSION     XP_077502040.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645914.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..156
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..156
                     /product="uncharacterized protein LOC144113031 isoform X2"
                     /calculated_mol_wt=17431
     Region          6..97
                     /region_name="FHA"
                     /note="forkhead associated (FHA) domain superfamily;
                     cl00062"
                     /db_xref="CDD:469597"
     Site            order(30..31,45,46,66..67)
                     /site_type="other"
                     /note="phosphopeptide binding site [polypeptide binding]"
                     /db_xref="CDD:438714"
     CDS             1..156
                     /gene="LOC144113031"
                     /coded_by="XM_077645914.1:195..665"
                     /db_xref="GeneID:144113031"
ORIGIN      
        1 mpwyprifti kkdgtrgskh rfqrgrcsvg taagcdihir gadssyclfl vdknmlaqvv
       61 nvtgsagvkh ngervrntsf lshgdivqig srqfrfewvn krkkksaqkr tkksskvaaa
      121 shqpkewadd kpdasrrtae dhseeappak rarkkv
//
LOCUS       XP_077502041            2324 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113032 [Amblyomma americanum].
ACCESSION   XP_077502041
VERSION     XP_077502041.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645915.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2324
                     /product="uncharacterized protein LOC144113032"
                     /calculated_mol_wt=247485
     Region          6..97
                     /region_name="FHA_Ki67"
                     /note="forkhead associated (FHA) domain found in
                     proliferation marker protein Ki-67 and similar proteins;
                     cd22673"
                     /db_xref="CDD:438725"
     Site            order(9..13,18..21,23..24,31..45,65..66,90..92,94)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:438725"
     Site            order(31,42..43,65..66,90)
                     /site_type="other"
                     /note="phosphopeptide binding site [polypeptide binding]"
                     /db_xref="CDD:438725"
     Region          <1948..>2323
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..2324
                     /gene="LOC144113032"
                     /coded_by="XM_077645915.1:184..7158"
                     /db_xref="GeneID:144113032"
ORIGIN      
        1 mpwypkivtv kkdgtrgsey pfptgrcsvg giagcdihir gagsshclfi vdknqqahvv
       61 nvtgsadvkh ngedvdnesf lghgdivqig prqfrfewve krkkeesskv aaashqpkaa
      121 gdaresstlh neidneahhv eqsngtpgac tptaysfdqs lfktprippe mfvsplsatd
      181 sdgcngdkgg pgtdalgnsq hnssssstpl idepriralh sstsstplid eprtrttrsr
      241 sslvgmvnsf reqehsafqe rqsysslkrk sqshqcsvee gpsklrrsds cghaqrtpla
      301 chsnangqsg pssrsssteg caahidgstl pgtsaqffaa stpvtraarn srlrsdkslk
      361 gisfshqekn sgfvtpgsqs sypngnattd esfldspgls rralrssras lshtslsssa
      421 dslgceslfk tpshtdsisk telgpltaqd isncslgnkr vhkrlshvan essqqsqaks
      481 ftaspmssrs rhslekntng aqtpsdhsar stransslgq nsestssvkr vvtadetiss
      541 fdgsncegqf gastlgfleq eegvsakktg skraistdst ssfkespgdk ggtaevssra
      601 arssitnsav dsptwvelgs egllqgvfcv saddskrvtr strlshtpnq tpasnnkskk
      661 aspeqegava ceegddeeeg vpvqsarems qsdqeslehm givaespkvt vskqtiqtle
      721 nsresaleka tlltrsgrfs htptskemre evseleatgt asalkaptsr rtlrssnvse
      781 emnravstls pghmtlddvd aavkgfnkrt rsarfshelv srkadekase hkrggmsptl
      841 qssgaqqali skkgiegmlk qktagtpksl kalaarqvpr ssevnekeaa ntllskrmss
      901 qnmgvalees fgctrstrss ctpasvsphv ekleqqiegt pvavkgtkgk qtavsklafg
      961 gvskqkrdsa vlggektptl lkadeerspe qetldmqtsk tlsspksdct pasrsgdrgt
     1021 pgpdevgsqd pmggsetrqa pvsrknkalt setlntpkss kalgskrtpa sgkidvsnea
     1081 teegivgtpa aaecapfmst gsvmnesfrr sarssctpvl eqepntesif npleasmarq
     1141 sipgnmksea dtpkfqkvag vkrtsaakmt tqergdlkqt trassakrvs fddtcvvveg
     1201 fvrstrssls sasrnvnaat pgmeevcsqn vmgdsivsrr skealaqetv dtpkslktsg
     1261 gkqtpasskm saseeateqd imgtspvers plmsispvva esvgtqstrs srtptlknss
     1321 keapgdetvg ltpnslkasg qkkavsrkvs netpqqemad tpnslkasaa krtsaskkii
     1381 enqgdlkqtt laspsecvsf dntsaategf vrstrpslss asrnvnavtp gmeevclqnv
     1441 mgasrvsrrs kealaqekvd tpkslktsgg kqtpasskis aseeateqdi mgtssversp
     1501 lmsispvvae svgtqstrss rtptvknssk eapgdeivgl apnslkasgq kkavsrkvnn
     1561 etpqqemadt pkslkasaak risaskkiie dqgdlkqttr aspsecvsfd ntsaategfv
     1621 rstrsslssa srnvnavtpg meevgsqnvm gasrvsrrsk ealaqetvdt pkslktsgrk
     1681 qtpasskisa seeateqnim gaspvespky leastaqwas paknvveedl eqetgaspsk
     1741 qvfsnntcvv seesvrptrs grtpasrnii tetpgqeevh lqeatrgsga krpavsrknk
     1801 kasaqkpvgt pksskalgvk qvqpsdevia dggtleqdta gmapdeyvtf dntdsaveas
     1861 drtrsirssq tssnegklkv tekekeatag snqkpsskps rakrtptskr ataadvatel
     1921 ekagmsplqh vafdntdgta aasgratraa rpkyaendkr tdvkmprqea ectvsplkal
     1981 eaaqtlasnk elgespklea vdtkktsrki lqaktarsrr kvnegeevpe letagvspsk
     2041 cssathvhal adeparptrg crtrgsrkdn veapeekevd hpqstkatra khtqgsrkvc
     2101 kkdetlqdva gsslvtsstt qrkdatfdkh lqgelsptke vsvtrntrgk rkvdlkkesi
     2161 aekqttaeel igtvaekrgr rqqrvasaed aqnpdsteqi inpkitrqrr anggakkapr
     2221 nkevvddapa spnveeatve tastegkvla ttmepkrskr kmaavkvdma qtateatprk
     2281 trgrrakale tviewaddkp dasrrtakdh seevplpkrt rkkv
//
LOCUS       XP_077502042            2324 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113032 [Amblyomma americanum].
ACCESSION   XP_077502042
VERSION     XP_077502042.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645916.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2324
                     /product="uncharacterized protein LOC144113032"
                     /calculated_mol_wt=247485
     Region          6..97
                     /region_name="FHA_Ki67"
                     /note="forkhead associated (FHA) domain found in
                     proliferation marker protein Ki-67 and similar proteins;
                     cd22673"
                     /db_xref="CDD:438725"
     Site            order(9..13,18..21,23..24,31..45,65..66,90..92,94)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:438725"
     Site            order(31,42..43,65..66,90)
                     /site_type="other"
                     /note="phosphopeptide binding site [polypeptide binding]"
                     /db_xref="CDD:438725"
     Region          <1948..>2323
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..2324
                     /gene="LOC144113032"
                     /coded_by="XM_077645916.1:229..7203"
                     /db_xref="GeneID:144113032"
ORIGIN      
        1 mpwypkivtv kkdgtrgsey pfptgrcsvg giagcdihir gagsshclfi vdknqqahvv
       61 nvtgsadvkh ngedvdnesf lghgdivqig prqfrfewve krkkeesskv aaashqpkaa
      121 gdaresstlh neidneahhv eqsngtpgac tptaysfdqs lfktprippe mfvsplsatd
      181 sdgcngdkgg pgtdalgnsq hnssssstpl idepriralh sstsstplid eprtrttrsr
      241 sslvgmvnsf reqehsafqe rqsysslkrk sqshqcsvee gpsklrrsds cghaqrtpla
      301 chsnangqsg pssrsssteg caahidgstl pgtsaqffaa stpvtraarn srlrsdkslk
      361 gisfshqekn sgfvtpgsqs sypngnattd esfldspgls rralrssras lshtslsssa
      421 dslgceslfk tpshtdsisk telgpltaqd isncslgnkr vhkrlshvan essqqsqaks
      481 ftaspmssrs rhslekntng aqtpsdhsar stransslgq nsestssvkr vvtadetiss
      541 fdgsncegqf gastlgfleq eegvsakktg skraistdst ssfkespgdk ggtaevssra
      601 arssitnsav dsptwvelgs egllqgvfcv saddskrvtr strlshtpnq tpasnnkskk
      661 aspeqegava ceegddeeeg vpvqsarems qsdqeslehm givaespkvt vskqtiqtle
      721 nsresaleka tlltrsgrfs htptskemre evseleatgt asalkaptsr rtlrssnvse
      781 emnravstls pghmtlddvd aavkgfnkrt rsarfshelv srkadekase hkrggmsptl
      841 qssgaqqali skkgiegmlk qktagtpksl kalaarqvpr ssevnekeaa ntllskrmss
      901 qnmgvalees fgctrstrss ctpasvsphv ekleqqiegt pvavkgtkgk qtavsklafg
      961 gvskqkrdsa vlggektptl lkadeerspe qetldmqtsk tlsspksdct pasrsgdrgt
     1021 pgpdevgsqd pmggsetrqa pvsrknkalt setlntpkss kalgskrtpa sgkidvsnea
     1081 teegivgtpa aaecapfmst gsvmnesfrr sarssctpvl eqepntesif npleasmarq
     1141 sipgnmksea dtpkfqkvag vkrtsaakmt tqergdlkqt trassakrvs fddtcvvveg
     1201 fvrstrssls sasrnvnaat pgmeevcsqn vmgdsivsrr skealaqetv dtpkslktsg
     1261 gkqtpasskm saseeateqd imgtspvers plmsispvva esvgtqstrs srtptlknss
     1321 keapgdetvg ltpnslkasg qkkavsrkvs netpqqemad tpnslkasaa krtsaskkii
     1381 enqgdlkqtt laspsecvsf dntsaategf vrstrpslss asrnvnavtp gmeevclqnv
     1441 mgasrvsrrs kealaqekvd tpkslktsgg kqtpasskis aseeateqdi mgtssversp
     1501 lmsispvvae svgtqstrss rtptvknssk eapgdeivgl apnslkasgq kkavsrkvnn
     1561 etpqqemadt pkslkasaak risaskkiie dqgdlkqttr aspsecvsfd ntsaategfv
     1621 rstrsslssa srnvnavtpg meevgsqnvm gasrvsrrsk ealaqetvdt pkslktsgrk
     1681 qtpasskisa seeateqnim gaspvespky leastaqwas paknvveedl eqetgaspsk
     1741 qvfsnntcvv seesvrptrs grtpasrnii tetpgqeevh lqeatrgsga krpavsrknk
     1801 kasaqkpvgt pksskalgvk qvqpsdevia dggtleqdta gmapdeyvtf dntdsaveas
     1861 drtrsirssq tssnegklkv tekekeatag snqkpsskps rakrtptskr ataadvatel
     1921 ekagmsplqh vafdntdgta aasgratraa rpkyaendkr tdvkmprqea ectvsplkal
     1981 eaaqtlasnk elgespklea vdtkktsrki lqaktarsrr kvnegeevpe letagvspsk
     2041 cssathvhal adeparptrg crtrgsrkdn veapeekevd hpqstkatra khtqgsrkvc
     2101 kkdetlqdva gsslvtsstt qrkdatfdkh lqgelsptke vsvtrntrgk rkvdlkkesi
     2161 aekqttaeel igtvaekrgr rqqrvasaed aqnpdsteqi inpkitrqrr anggakkapr
     2221 nkevvddapa spnveeatve tastegkvla ttmepkrskr kmaavkvdma qtateatprk
     2281 trgrrakale tviewaddkp dasrrtakdh seevplpkrt rkkv
//
LOCUS       XP_077502043             293 aa            linear   INV 25-NOV-2025
DEFINITION  docking protein 1-like [Amblyomma americanum].
ACCESSION   XP_077502043
VERSION     XP_077502043.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645917.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..293
                     /product="docking protein 1-like"
                     /calculated_mol_wt=32285
     Region          8..110
                     /region_name="PH"
                     /note="Pleckstrin homology domain; smart00233"
                     /db_xref="CDD:214574"
     Region          140..240
                     /region_name="IRS"
                     /note="PTB domain (IRS-1 type); pfam02174"
                     /db_xref="CDD:460473"
     CDS             1..293
                     /gene="LOC144113033"
                     /coded_by="XM_077645917.1:154..1035"
                     /db_xref="GeneID:144113033"
ORIGIN      
        1 mdgeepaktg ylhvlhqgfl kkswlhkyca lydssrhgvk rfevydsees fsrhaqqkci
       61 tledcikvvp alqknqpnvf evhtrtlcqh fsadsfgemt qwieairgvs fgqqavsnpa
      121 tqdspvveee ntlygsldap hvfrvralpt eagercgllh gsffllvgpr gvtlaergpq
      181 gslgpallww pyysirrygl lagtfsleag rkaasgeglf swhaadaedi fravascvra
      241 sadgsalldq leqhcyenvp mspapaaded avphvrrnga eyavvhkpgr lfq
//
LOCUS       XP_077502044             608 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113035 [Amblyomma americanum].
ACCESSION   XP_077502044
VERSION     XP_077502044.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645918.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..608
                     /product="uncharacterized protein LOC144113035"
                     /calculated_mol_wt=67775
     CDS             1..608
                     /gene="LOC144113035"
                     /coded_by="XM_077645918.1:119..1945"
                     /db_xref="GeneID:144113035"
ORIGIN      
        1 maaegqcssa dqaesdgsdl hgqnagpvep dehdlvyvpd nakcpegirs qlrawavkhh
       61 iriaavnsll dilrprftdl lktqsalvst rqsvpleqlg sgeyvhfglq sgllraldtd
      121 gvplfkssct qvrpilvqvh hprskqsdii fpvgifcgts kptfldeylk afieemcpll
      181 sngilhksia ykvvlraiic dapakafvka tklfsgyygc dkcctkgvyl grityqsmds
      241 plrtdqsfad qtqaehhkgi spltsigipm vtrfpldymh lvclgvvrkl igywlrgplk
      301 mrlahfsvmq lsghlatkrq hitsdfarkl ravdifeqwk atefrqflly sgpvvakkil
      361 pramyehflq lhvgiailss ssmdyfidya dqllrsfvsk cksiygkesl iynvhglihl
      421 andcrqlgcl dnfscfafen ylkdlkahvr cgkkpleqly sriieeqacs vvgngssaef
      481 sayevlhssc hssgpalsyp alnqyqkvtw resvirtkqp dnclllsgnr ivfasnflls
      541 gseilivgkl cksegdfytc pcrsslpsvf kvssrgsnle vflfsdvckg qllpfkgkdv
      601 ffplfyll
//
LOCUS       XP_077502045             316 aa            linear   INV 25-NOV-2025
DEFINITION  phosphoseryl tRNA kinase isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502045
VERSION     XP_077502045.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645919.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..316
                     /product="phosphoseryl tRNA kinase isoform X1"
                     /calculated_mol_wt=35074
     Region          9..287
                     /region_name="NK"
                     /note="Nucleoside/nucleotide kinase (NK) is a protein
                     superfamily consisting of multiple families of enzymes
                     that share structural similarity and are functionally
                     related to the catalysis of the reversible phosphate group
                     transfer from nucleoside triphosphates...; cl17190"
                     /db_xref="CDD:450170"
     CDS             1..316
                     /gene="Pstk"
                     /coded_by="XM_077645919.1:414..1364"
                     /db_xref="GeneID:144113036"
ORIGIN      
        1 mgtgcasvlm lfcglpasgk ttlvqkiraa lgsrsstsar glswcpyvic ydelvstevq
       61 aemasrkglt kecrqhllea adcyigqlcg dnfepshvye epvnemvgrl laatrqiqpn
      121 ekavimvddn lyyrsmraaw fklarnyslg fcqvfllcte eeavrrnasr afsvpestir
      181 tmakkfeapr depweqltvt vdgtpdsiek vvaileaasr qpwppvitsp irepvpasqm
      241 hiwdlelrai vskfirqaqg ekssksqvqs wsvkvqkarq dvlaylkgld fgserkemnl
      301 eelmqahlgs stgdgs
//
LOCUS       XP_077502046             299 aa            linear   INV 25-NOV-2025
DEFINITION  phosphoseryl tRNA kinase isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502046
VERSION     XP_077502046.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645920.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..299
                     /product="phosphoseryl tRNA kinase isoform X2"
                     /calculated_mol_wt=33144
     Region          9..>254
                     /region_name="NK"
                     /note="Nucleoside/nucleotide kinase (NK) is a protein
                     superfamily consisting of multiple families of enzymes
                     that share structural similarity and are functionally
                     related to the catalysis of the reversible phosphate group
                     transfer from nucleoside triphosphates...; cl17190"
                     /db_xref="CDD:450170"
     CDS             1..299
                     /gene="Pstk"
                     /coded_by="XM_077645920.1:152..1051"
                     /db_xref="GeneID:144113036"
ORIGIN      
        1 mgtgcasvlm lfcglpasgk ttlvqkiraa lgsrsstsar glswcpyvic ydelvstevq
       61 aemasrkglt kecrqhllea adcyigqlcg dnfepshvye epvnemvgrl laatrqiqpn
      121 ekavimvddn lyyrsmraaw fklarnyslg fcqvfllcte eeavrrnasr afsvpestir
      181 tmakkfeapr depweqltvt vdgtpdsmpc tdeehvrppm nrgnatrgtl rclldvdgil
      241 lsssthdrdi slrrervmna tvnfsgyafl grpglrlgis gkrnpiklgt khgrvapyh
//
LOCUS       XP_077502048             255 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113037 [Amblyomma americanum].
ACCESSION   XP_077502048
VERSION     XP_077502048.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645922.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..255
                     /product="uncharacterized protein LOC144113037"
                     /calculated_mol_wt=28358
     CDS             1..255
                     /gene="LOC144113037"
                     /coded_by="XM_077645922.1:141..908"
                     /db_xref="GeneID:144113037"
ORIGIN      
        1 mttvpsdlyv grfhiveftd egstyvvpes wvrmeadgat fywppttakk evdrmillav
       61 pveddwegyk arilatksty erakeclpha imtshldseg svvekkrrrk rkavllsdse
      121 sddilevrsp gtvmkrgkgs gmsqdvqehi pirrnsseqv hirdgfvaar erqvprvlsk
      181 spttfnrhtk qlpgmsrlse rfakhgaglq dkattsswgq dssssdceaa pantsapkea
      241 sslpawftal ekesf
//
LOCUS       XP_077502049             441 aa            linear   INV 25-NOV-2025
DEFINITION  eukaryotic release factor 1 [Amblyomma americanum].
ACCESSION   XP_077502049
VERSION     XP_077502049.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645923.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..441
                     /product="eukaryotic release factor 1"
                     /calculated_mol_wt=49328
     Region          18..422
                     /region_name="aRF1/eRF1"
                     /note="peptide chain release factor 1, archaeal and
                     eukaryotic forms; TIGR03676"
                     /db_xref="CDD:274719"
     CDS             1..441
                     /gene="eRF1"
                     /coded_by="XM_077645923.1:305..1630"
                     /db_xref="GeneID:144113038"
ORIGIN      
        1 mastgynlee snadrnveiw kikkliksle aargngtsmi sliippkdqi srvskmlade
       61 fgtasniksr vnrlsvlgai tsvqhrlkly tkvppnglvi ycgtivteeg kekkvnidfe
      121 pfkpintsly lcdnkfhtea lsalladdnk fgfivmdgng alfgtlqgnt revlhkftvd
      181 lpkkhgrggq salrfarlrm ekrhnyvrkv aevattlyis ndrpniagli lagsadfkte
      241 lsqsdmfdpr lqvkvlklvd vsyggengfn qaielsaevl snvkfiqekk ligryfdeis
      301 qdtgkycfgv edtlkalemg sveiliawen ldivryvlkn htsdeekilh ltpeqekdks
      361 hfldretgve lelvesmall ewlannyknf gatleiitdk sqegsqfvkg fggiggilry
      421 rvdfqslevn dlvddfdldd l
//
LOCUS       XP_077502050             396 aa            linear   INV 25-NOV-2025
DEFINITION  pantothenate kinase 3 fbl isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502050
VERSION     XP_077502050.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645924.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..396
                     /product="pantothenate kinase 3 fbl isoform X1"
                     /calculated_mol_wt=43320
     Region          46..389
                     /region_name="ASKHA_NBD_PanK-II_Pank1-like"
                     /note="nucleotide-binding domain (NBD) of the pantothenate
                     kinase 1 (Pank1)-like subfamily; cd24122"
                     /db_xref="CDD:466972"
     CDS             1..396
                     /gene="fbl"
                     /coded_by="XM_077645924.1:422..1612"
                     /db_xref="GeneID:144113040"
ORIGIN      
        1 mslsdwsaav prtgldsdgr ravmasvngd atpaarvpsp qppmpwfgmd iggtlvklvy
       61 fepavedgpe ttealerikh ylkknaaygq sgkrdvhlqm dgvevggieg tlhfirfpts
      121 emprflelak akgiaslast vcatgggahk fehdfrtevg lglhkfdemd slirgihyme
      181 ahnsrecyyy sspmercvkm pydfsnpypy lvvnigsgvs ilavrsptey hrvtgtslgg
      241 gtflglccll tgcetfeeai elaqrgdsth vdklvrdiyg gdypkfdlra dtvassfgnm
      301 ncrarreaas redlaratlv titnnigsia rmcainegis rvvfvgnflr vnpismrmla
      361 yamqfwskgt lkalfleheg yfgavgclle lmktcs
//
LOCUS       XP_077502051             396 aa            linear   INV 25-NOV-2025
DEFINITION  pantothenate kinase 3 fbl isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502051
VERSION     XP_077502051.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645925.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..396
                     /product="pantothenate kinase 3 fbl isoform X1"
                     /calculated_mol_wt=43320
     Region          46..389
                     /region_name="ASKHA_NBD_PanK-II_Pank1-like"
                     /note="nucleotide-binding domain (NBD) of the pantothenate
                     kinase 1 (Pank1)-like subfamily; cd24122"
                     /db_xref="CDD:466972"
     CDS             1..396
                     /gene="fbl"
                     /coded_by="XM_077645925.1:342..1532"
                     /db_xref="GeneID:144113040"
ORIGIN      
        1 mslsdwsaav prtgldsdgr ravmasvngd atpaarvpsp qppmpwfgmd iggtlvklvy
       61 fepavedgpe ttealerikh ylkknaaygq sgkrdvhlqm dgvevggieg tlhfirfpts
      121 emprflelak akgiaslast vcatgggahk fehdfrtevg lglhkfdemd slirgihyme
      181 ahnsrecyyy sspmercvkm pydfsnpypy lvvnigsgvs ilavrsptey hrvtgtslgg
      241 gtflglccll tgcetfeeai elaqrgdsth vdklvrdiyg gdypkfdlra dtvassfgnm
      301 ncrarreaas redlaratlv titnnigsia rmcainegis rvvfvgnflr vnpismrmla
      361 yamqfwskgt lkalfleheg yfgavgclle lmktcs
//
LOCUS       XP_077502052             396 aa            linear   INV 25-NOV-2025
DEFINITION  pantothenate kinase 3 fbl isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502052
VERSION     XP_077502052.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645926.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..396
                     /product="pantothenate kinase 3 fbl isoform X1"
                     /calculated_mol_wt=43320
     Region          46..389
                     /region_name="ASKHA_NBD_PanK-II_Pank1-like"
                     /note="nucleotide-binding domain (NBD) of the pantothenate
                     kinase 1 (Pank1)-like subfamily; cd24122"
                     /db_xref="CDD:466972"
     CDS             1..396
                     /gene="fbl"
                     /coded_by="XM_077645926.1:366..1556"
                     /db_xref="GeneID:144113040"
ORIGIN      
        1 mslsdwsaav prtgldsdgr ravmasvngd atpaarvpsp qppmpwfgmd iggtlvklvy
       61 fepavedgpe ttealerikh ylkknaaygq sgkrdvhlqm dgvevggieg tlhfirfpts
      121 emprflelak akgiaslast vcatgggahk fehdfrtevg lglhkfdemd slirgihyme
      181 ahnsrecyyy sspmercvkm pydfsnpypy lvvnigsgvs ilavrsptey hrvtgtslgg
      241 gtflglccll tgcetfeeai elaqrgdsth vdklvrdiyg gdypkfdlra dtvassfgnm
      301 ncrarreaas redlaratlv titnnigsia rmcainegis rvvfvgnflr vnpismrmla
      361 yamqfwskgt lkalfleheg yfgavgclle lmktcs
//
LOCUS       XP_077502053             373 aa            linear   INV 25-NOV-2025
DEFINITION  pantothenate kinase 3 fbl isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502053
VERSION     XP_077502053.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645927.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..373
                     /product="pantothenate kinase 3 fbl isoform X2"
                     /calculated_mol_wt=40890
     Region          23..366
                     /region_name="ASKHA_NBD_PanK-II_Pank1-like"
                     /note="nucleotide-binding domain (NBD) of the pantothenate
                     kinase 1 (Pank1)-like subfamily; cd24122"
                     /db_xref="CDD:466972"
     CDS             1..373
                     /gene="fbl"
                     /coded_by="XM_077645927.1:179..1300"
                     /db_xref="GeneID:144113040"
ORIGIN      
        1 masvngdatp aarvpspqpp mpwfgmdigg tlvklvyfep avedgpette alerikhylk
       61 knaaygqsgk rdvhlqmdgv evggiegtlh firfptsemp rflelakakg iaslastvca
      121 tgggahkfeh dfrtevglgl hkfdemdsli rgihymeahn srecyyyssp mercvkmpyd
      181 fsnpypylvv nigsgvsila vrspteyhrv tgtslgggtf lglcclltgc etfeeaiela
      241 qrgdsthvdk lvrdiyggdy pkfdlradtv assfgnmncr arreaasred laratlvtit
      301 nnigsiarmc ainegisrvv fvgnflrvnp ismrmlayam qfwskgtlka lflehegyfg
      361 avgcllelmk tcs
//
LOCUS       XP_077502054             373 aa            linear   INV 25-NOV-2025
DEFINITION  pantothenate kinase 3 fbl isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502054
VERSION     XP_077502054.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645928.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..373
                     /product="pantothenate kinase 3 fbl isoform X2"
                     /calculated_mol_wt=40890
     Region          23..366
                     /region_name="ASKHA_NBD_PanK-II_Pank1-like"
                     /note="nucleotide-binding domain (NBD) of the pantothenate
                     kinase 1 (Pank1)-like subfamily; cd24122"
                     /db_xref="CDD:466972"
     CDS             1..373
                     /gene="fbl"
                     /coded_by="XM_077645928.1:165..1286"
                     /db_xref="GeneID:144113040"
ORIGIN      
        1 masvngdatp aarvpspqpp mpwfgmdigg tlvklvyfep avedgpette alerikhylk
       61 knaaygqsgk rdvhlqmdgv evggiegtlh firfptsemp rflelakakg iaslastvca
      121 tgggahkfeh dfrtevglgl hkfdemdsli rgihymeahn srecyyyssp mercvkmpyd
      181 fsnpypylvv nigsgvsila vrspteyhrv tgtslgggtf lglcclltgc etfeeaiela
      241 qrgdsthvdk lvrdiyggdy pkfdlradtv assfgnmncr arreaasred laratlvtit
      301 nnigsiarmc ainegisrvv fvgnflrvnp ismrmlayam qfwskgtlka lflehegyfg
      361 avgcllelmk tcs
//
LOCUS       XP_077502055             616 aa            linear   INV 25-NOV-2025
DEFINITION  cytoplasmic polyadenylation element-binding protein orb2 isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077502055
VERSION     XP_077502055.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645929.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..616
                     /product="cytoplasmic polyadenylation element-binding
                     protein orb2 isoform X1"
                     /calculated_mol_wt=66840
     Region          359..450
                     /region_name="RRM1_CPEB2_like"
                     /note="RNA recognition motif 1 (RRM1) found in cytoplasmic
                     polyadenylation element-binding protein CPEB-2, CPEB-3,
                     CPEB-4 and similar protiens; cd12724"
                     /db_xref="CDD:410123"
     Region          467..547
                     /region_name="RRM2_CPEB2_like"
                     /note="RNA recognition motif 2 (RRM2) found in cytoplasmic
                     polyadenylation element-binding protein CPEB-2, CPEB-3,
                     CPEB-4 and similar protiens; cd12726"
                     /db_xref="CDD:410125"
     Region          542..603
                     /region_name="CEBP_ZZ"
                     /note="Cytoplasmic polyadenylation element-binding protein
                     ZZ domain; pfam16366"
                     /db_xref="CDD:465105"
     CDS             1..616
                     /gene="orb2"
                     /coded_by="XM_077645929.1:98..1948"
                     /db_xref="GeneID:144113041"
ORIGIN      
        1 mgdytfsvsa nvralspvef qpnpgnsalf fspdqightm qddllleksa gkqassqlss
       61 asvettsnge qaledyssrk skgedpaaan slpektespd vvpsplqmgf sseviatsga
      121 nfwsavaade afiqgiptvn gtlafpsfps pmlgaghpqr raitgahnfa aptrgpppqg
      181 pplfkayssw sspqqpswss ppwsglkrsv pnlgplspag kespkfrrss slpkpdedgf
      241 qvaqgpldtl rfpleqqlld mvrqdppeaa hyckpwgglg gaasslgsaa aypefrpdea
      301 rlqarggrgg fsrrkgragg gpwmlagaks pffldgdlvl eeqrcspkgg epaaerysrk
      361 vfvgglppdi deeeitasfr rfgplvvdwp hkaesksyfp pkgyafllfq decsvqalmd
      421 aciqeedkly lcvssptikd kpvqirpwrl gdadfvldas lpldprktvf vggvprplka
      481 velamimdrl yggvcyagid tdpelkypkg agrvafsnqq syiaaisarf vqlqhgdiek
      541 rvevkpyvld dqmcdecqga rcggkfapff canvtclqyy cehcwatihs rpgrefhkpl
      601 vkegadrpra vpfrwc
//
LOCUS       XP_077502056             577 aa            linear   INV 25-NOV-2025
DEFINITION  cytoplasmic polyadenylation element-binding protein orb2 isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077502056
VERSION     XP_077502056.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645930.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..577
                     /product="cytoplasmic polyadenylation element-binding
                     protein orb2 isoform X2"
                     /calculated_mol_wt=62658
     Region          320..411
                     /region_name="RRM1_CPEB2_like"
                     /note="RNA recognition motif 1 (RRM1) found in cytoplasmic
                     polyadenylation element-binding protein CPEB-2, CPEB-3,
                     CPEB-4 and similar protiens; cd12724"
                     /db_xref="CDD:410123"
     Region          428..508
                     /region_name="RRM2_CPEB2_like"
                     /note="RNA recognition motif 2 (RRM2) found in cytoplasmic
                     polyadenylation element-binding protein CPEB-2, CPEB-3,
                     CPEB-4 and similar protiens; cd12726"
                     /db_xref="CDD:410125"
     Region          503..564
                     /region_name="CEBP_ZZ"
                     /note="Cytoplasmic polyadenylation element-binding protein
                     ZZ domain; pfam16366"
                     /db_xref="CDD:465105"
     CDS             1..577
                     /gene="orb2"
                     /coded_by="XM_077645930.1:346..2079"
                     /db_xref="GeneID:144113041"
ORIGIN      
        1 mqddllleks agkqassqls sasvettsng eqaledyssr kskgedpaaa nslpektesp
       61 dvvpsplqmg fsseviatsg anfwsavaad eafiqgiptv ngtlafpsfp spmlgaghpq
      121 rraitgahnf aaptrgpppq gpplfkayss wsspqqpsws sppwsglkrs vpnlgplspa
      181 gkespkfrrs sslpkpdedg fqvaqgpldt lrfpleqqll dmvrqdppea ahyckpwggl
      241 ggaasslgsa aaypefrpde arlqarggrg gfsrrkgrag ggpwmlagak spffldgdlv
      301 leeqrcspkg gepaaerysr kvfvgglppd ideeeitasf rrfgplvvdw phkaesksyf
      361 ppkgyafllf qdecsvqalm daciqeedkl ylcvssptik dkpvqirpwr lgdadfvlda
      421 slpldprktv fvggvprplk avelamimdr lyggvcyagi dtdpelkypk gagrvafsnq
      481 qsyiaaisar fvqlqhgdie krvevkpyvl ddqmcdecqg arcggkfapf fcanvtclqy
      541 ycehcwatih srpgrefhkp lvkegadrpr avpfrwc
//
LOCUS       XP_077502057             577 aa            linear   INV 25-NOV-2025
DEFINITION  cytoplasmic polyadenylation element-binding protein orb2 isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077502057
VERSION     XP_077502057.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645931.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..577
                     /product="cytoplasmic polyadenylation element-binding
                     protein orb2 isoform X2"
                     /calculated_mol_wt=62658
     Region          320..411
                     /region_name="RRM1_CPEB2_like"
                     /note="RNA recognition motif 1 (RRM1) found in cytoplasmic
                     polyadenylation element-binding protein CPEB-2, CPEB-3,
                     CPEB-4 and similar protiens; cd12724"
                     /db_xref="CDD:410123"
     Region          428..508
                     /region_name="RRM2_CPEB2_like"
                     /note="RNA recognition motif 2 (RRM2) found in cytoplasmic
                     polyadenylation element-binding protein CPEB-2, CPEB-3,
                     CPEB-4 and similar protiens; cd12726"
                     /db_xref="CDD:410125"
     Region          503..564
                     /region_name="CEBP_ZZ"
                     /note="Cytoplasmic polyadenylation element-binding protein
                     ZZ domain; pfam16366"
                     /db_xref="CDD:465105"
     CDS             1..577
                     /gene="orb2"
                     /coded_by="XM_077645931.1:339..2072"
                     /db_xref="GeneID:144113041"
ORIGIN      
        1 mqddllleks agkqassqls sasvettsng eqaledyssr kskgedpaaa nslpektesp
       61 dvvpsplqmg fsseviatsg anfwsavaad eafiqgiptv ngtlafpsfp spmlgaghpq
      121 rraitgahnf aaptrgpppq gpplfkayss wsspqqpsws sppwsglkrs vpnlgplspa
      181 gkespkfrrs sslpkpdedg fqvaqgpldt lrfpleqqll dmvrqdppea ahyckpwggl
      241 ggaasslgsa aaypefrpde arlqarggrg gfsrrkgrag ggpwmlagak spffldgdlv
      301 leeqrcspkg gepaaerysr kvfvgglppd ideeeitasf rrfgplvvdw phkaesksyf
      361 ppkgyafllf qdecsvqalm daciqeedkl ylcvssptik dkpvqirpwr lgdadfvlda
      421 slpldprktv fvggvprplk avelamimdr lyggvcyagi dtdpelkypk gagrvafsnq
      481 qsyiaaisar fvqlqhgdie krvevkpyvl ddqmcdecqg arcggkfapf fcanvtclqy
      541 ycehcwatih srpgrefhkp lvkegadrpr avpfrwc
//
LOCUS       XP_077502058             272 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase MARCHF5 [Amblyomma americanum].
ACCESSION   XP_077502058
VERSION     XP_077502058.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645932.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..272
                     /product="E3 ubiquitin-protein ligase MARCHF5"
                     /calculated_mol_wt=30057
     Region          18..78
                     /region_name="RING_CH-C4HC3_MARCH5"
                     /note="RING-CH finger, H2 subclass (C4HC3-type), found in
                     membrane-associated RING-CH5 (MARCH5); cd16701"
                     /db_xref="CDD:438361"
     CDS             1..272
                     /gene="LOC144113043"
                     /coded_by="XM_077645932.1:267..1085"
                     /db_xref="GeneID:144113043"
ORIGIN      
        1 mvsatmsaas tdgscerryc wvcfasedee rdaawvqpck crgttkwvhq aclqrwidek
       61 qggnsaakva cpqcsseyvv vlpslggpvy lldlmdrsvy racpfvaagm lmgsvywtav
      121 tygavtvmqv lghkqglsvm eqadplfllv glpsipvvlv larmvrwedy llrvwcrrsa
      181 pfakrllfvv ppqdplsatr llcgalllpt aaslvgrlff grlapsqrtl lgglafvalk
      241 gaakiylkrh qylrqcrrhv ldyqqqqqss pp
//
LOCUS       XP_077502060             541 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113044 [Amblyomma americanum].
ACCESSION   XP_077502060
VERSION     XP_077502060.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645934.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..541
                     /product="uncharacterized protein LOC144113044"
                     /calculated_mol_wt=58984
     CDS             1..541
                     /gene="LOC144113044"
                     /coded_by="XM_077645934.1:130..1755"
                     /db_xref="GeneID:144113044"
ORIGIN      
        1 mfddhnkgen ergstficei gmlhdlvsga acsacgrcdl siresaatrk glaaflelyc
       61 qndacptavl sstysssris rsckgsdeas argsardafa vnvksvvaar aiglrheqll
      121 rfcaimgfak pvhrrafvav gkklhaaatk aaaenleqar qltaaeagst nvavmfdgtw
      181 qkrghkshng vgtaislltg lcldfevlsn yclscsrhka ledeeeqiwq ashtpvrekn
      241 atcsahamet eaalriwkrt dlyatplkyt tflsdgdska yaavaeldiy gsvpvqkedc
      301 tnhvakrlgi alrkaklprg eklkdktiak lqgyfqvatt snkgnvrdmy cavwasyfhs
      361 csrdgasshk fcpdgemswc khkralalgq papvhtpils vsqgkavlpi yerltdekll
      421 qrcvkgqtqn aaeslnskiw llcpktkfvt rtvvdtatai avlwfnkgha rfeevlqelg
      481 ilpsktllsl skevdkrris smstkataea rahchnvtkk arleesarrd cegptygagg
      541 f
//
LOCUS       XP_077502061             417 aa            linear   INV 25-NOV-2025
DEFINITION  C-terminal binding protein [Amblyomma americanum].
ACCESSION   XP_077502061
VERSION     XP_077502061.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645935.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..417
                     /product="C-terminal binding protein"
                     /calculated_mol_wt=45303
     Region          32..350
                     /region_name="CtBP_dh"
                     /note="C-terminal binding protein (CtBP),
                     D-isomer-specific 2-hydroxyacid dehydrogenases related
                     repressor; cd05299"
                     /db_xref="CDD:240624"
     Site            order(101,104..105,270,319,322)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:240624"
     Site            order(104,132,184..185,187..189,207..211,240..242,244,247,
                     268..270,294..295,319,321..322)
                     /site_type="other"
                     /note="NAD binding site [chemical binding]"
                     /db_xref="CDD:240624"
     CDS             1..417
                     /gene="CtBP"
                     /coded_by="XM_077645935.1:446..1699"
                     /db_xref="GeneID:144113045"
ORIGIN      
        1 mdkkkplkrp rmdnpplgar gpvangplha rplvalldgr dcsvempilk dvatvafcda
       61 qstqeihekv lneavgalmw htitltkedl ekfkalriiv rigsgvdnid tkaagelgia
      121 vcnvpgygve evadttlcli lnlyrrtywl anmvregkkf qgpeqvreaa qgcarirgdt
      181 lgivglgrvg tavalrarvf gfnvifydpy lpdgiekslg lsrvytlqdl lfqsdcvslh
      241 ctlnehnhhl ineytmkqmr pgaflvntar gglvdeaala aalkdgrira aaldvhenep
      301 yntlagplke apnlictpha awysdascte lremaaseir raivgripds lrncvnkeyf
      361 tgygdgingg aynynpmgvp hsttalepla pgvphstshl qdsanhlapk pessevh
//
LOCUS       XP_077502062             417 aa            linear   INV 25-NOV-2025
DEFINITION  C-terminal binding protein [Amblyomma americanum].
ACCESSION   XP_077502062
VERSION     XP_077502062.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645936.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..417
                     /product="C-terminal binding protein"
                     /calculated_mol_wt=45303
     Region          32..350
                     /region_name="CtBP_dh"
                     /note="C-terminal binding protein (CtBP),
                     D-isomer-specific 2-hydroxyacid dehydrogenases related
                     repressor; cd05299"
                     /db_xref="CDD:240624"
     Site            order(101,104..105,270,319,322)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:240624"
     Site            order(104,132,184..185,187..189,207..211,240..242,244,247,
                     268..270,294..295,319,321..322)
                     /site_type="other"
                     /note="NAD binding site [chemical binding]"
                     /db_xref="CDD:240624"
     CDS             1..417
                     /gene="CtBP"
                     /coded_by="XM_077645936.1:342..1595"
                     /db_xref="GeneID:144113045"
ORIGIN      
        1 mdkkkplkrp rmdnpplgar gpvangplha rplvalldgr dcsvempilk dvatvafcda
       61 qstqeihekv lneavgalmw htitltkedl ekfkalriiv rigsgvdnid tkaagelgia
      121 vcnvpgygve evadttlcli lnlyrrtywl anmvregkkf qgpeqvreaa qgcarirgdt
      181 lgivglgrvg tavalrarvf gfnvifydpy lpdgiekslg lsrvytlqdl lfqsdcvslh
      241 ctlnehnhhl ineytmkqmr pgaflvntar gglvdeaala aalkdgrira aaldvhenep
      301 yntlagplke apnlictpha awysdascte lremaaseir raivgripds lrncvnkeyf
      361 tgygdgingg aynynpmgvp hsttalepla pgvphstshl qdsanhlapk pessevh
//
LOCUS       XP_077502063             417 aa            linear   INV 25-NOV-2025
DEFINITION  C-terminal binding protein [Amblyomma americanum].
ACCESSION   XP_077502063
VERSION     XP_077502063.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645937.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..417
                     /product="C-terminal binding protein"
                     /calculated_mol_wt=45303
     Region          32..350
                     /region_name="CtBP_dh"
                     /note="C-terminal binding protein (CtBP),
                     D-isomer-specific 2-hydroxyacid dehydrogenases related
                     repressor; cd05299"
                     /db_xref="CDD:240624"
     Site            order(101,104..105,270,319,322)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:240624"
     Site            order(104,132,184..185,187..189,207..211,240..242,244,247,
                     268..270,294..295,319,321..322)
                     /site_type="other"
                     /note="NAD binding site [chemical binding]"
                     /db_xref="CDD:240624"
     CDS             1..417
                     /gene="CtBP"
                     /coded_by="XM_077645937.1:241..1494"
                     /db_xref="GeneID:144113045"
ORIGIN      
        1 mdkkkplkrp rmdnpplgar gpvangplha rplvalldgr dcsvempilk dvatvafcda
       61 qstqeihekv lneavgalmw htitltkedl ekfkalriiv rigsgvdnid tkaagelgia
      121 vcnvpgygve evadttlcli lnlyrrtywl anmvregkkf qgpeqvreaa qgcarirgdt
      181 lgivglgrvg tavalrarvf gfnvifydpy lpdgiekslg lsrvytlqdl lfqsdcvslh
      241 ctlnehnhhl ineytmkqmr pgaflvntar gglvdeaala aalkdgrira aaldvhenep
      301 yntlagplke apnlictpha awysdascte lremaaseir raivgripds lrncvnkeyf
      361 tgygdgingg aynynpmgvp hsttalepla pgvphstshl qdsanhlapk pessevh
//
LOCUS       XP_077502064             389 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein transferase Katazuke [Amblyomma americanum].
ACCESSION   XP_077502064
VERSION     XP_077502064.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645938.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..389
                     /product="E3 ubiquitin-protein transferase Katazuke"
                     /calculated_mol_wt=44209
     Region          106..137
                     /region_name="LisH"
                     /note="Lissencephaly type-1-like homology motif;
                     smart00667"
                     /db_xref="CDD:128913"
     Region          146..296
                     /region_name="CTLH"
                     /note="CTLH/CRA C-terminal to LisH motif domain;
                     pfam10607"
                     /db_xref="CDD:402305"
     Region          329..378
                     /region_name="RING-Ubox_Emp"
                     /note="U-box domain, a modified RING finger, found in
                     erythroblast macrophage protein (Emp) and similar
                     proteins; cd16659"
                     /db_xref="CDD:438321"
     CDS             1..389
                     /gene="Kaz"
                     /coded_by="XM_077645938.1:101..1270"
                     /db_xref="GeneID:144113046"
ORIGIN      
        1 msdikalehp tlkvpyeiln kkfraaqksm drevshvqsg aaelekslrd kapagqlhsq
       61 lgslveklel lrrksaesis eeleaaaack rrvehlkgfe tggeqwkrqr ldrmlvehll
      121 ragyygtaak laersglrdl tnmdlflvsk evedslasrd tskclawchd nksklrklrs
      181 tlefqlrqqe fvelvrrdrr leavrharrh fgaledeaql aevqrvmsll alpgarppdg
      241 rwellgdsrw elladsrwdl lvqqfrqeql rlyqlsscsv ltvalqagls alktpqcyde
      301 sqrnpdcpvc sralnclals lpyahcsqsr lvcrisgqpl nehnqplvlp ngfvygeqal
      361 ravarrgrvt cprsrdsfdl reaekvyvm
//
LOCUS       XP_077502065           17949 aa            linear   INV 25-NOV-2025
DEFINITION  sallimus [Amblyomma americanum].
ACCESSION   XP_077502065
VERSION     XP_077502065.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645939.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..17949
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..17949
                     /product="sallimus"
                     /calculated_mol_wt=2040862
     Region          126..341
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cl02488"
                     /db_xref="CDD:413338"
     Region          <214..>522
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Site            241..246
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          878..972
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          895..899
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          908..912
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          936..942
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          952..957
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          965..968
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1005..1093
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1023..1027
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1036..1040
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1059..1063
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1073..1078
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1086..1089
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1103..1193
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1121..1125
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1134..1138
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1159..1163
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1173..1178
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1186..1189
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1198..1288
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1215..1219
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1228..1232
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1254..1258
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1268..1273
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1281..1284
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1327..1420
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          1386..1390
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1400..1405
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1413..1416
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1641..1733
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1658..1662
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1671..1675
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1699..1703
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1713..1718
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1726..1729
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1768..1844
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1772..1776
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1785..1789
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1812..1816
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1826..1831
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1839..1842
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1934..2023
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1951..1955
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409473"
     Region          1964..1968
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409473"
     Region          1990..1993
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409473"
     Region          2003..2008
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409473"
     Region          2016..2019
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409473"
     Region          2051..2141
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2068..2072
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2081..2085
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2107..2111
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2121..2126
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2134..2137
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2157..2240
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2167..2171
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2180..2184
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2206..2210
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2220..2225
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2233..2236
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2297..2386
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2314..2318
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2329..2333
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2354..2358
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2368..2373
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2381..2384
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2431..2523
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2449..2453
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2460..2468
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2489..2493
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2503..2508
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2516..2519
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2571..2661
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2604..2608
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2629..2633
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2643..2648
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2705..2798
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2723..2727
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2738..2742
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2763..2767
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2777..2782
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2790..2793
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2840..2933
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2859..2863
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2872..2878
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2899..2903
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2913..2918
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2975..3064
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2992..2996
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3007..3011
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3033..3036
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3046..3051
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3059..3062
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3108..3199
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3125..3129
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3140..3144
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3165..3169
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3179..3184
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3193..3196
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3241..3331
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3258..3262
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3273..3277
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3298..3302
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3312..3317
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3325..3328
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3375..3466
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3392..3396
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3407..3411
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3432..3436
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3446..3451
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3459..3462
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3508..3600
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3526..3530
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3541..3545
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3566..3570
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3580..3585
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3593..3596
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3642..3734
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3660..3663
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3671..3679
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3714..3719
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3727..3730
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3774..3864
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3792..3796
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3807..3811
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3832..3836
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3846..3851
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3859..3862
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3907..3999
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3925..3929
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3936..3940
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3965..3969
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3979..3984
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3992..3995
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4041..4134
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          4075..4079
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4100..4104
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4114..4119
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4127..4130
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4175..4267
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          4193..4197
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4208..4212
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4234..4237
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4247..4252
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4260..4263
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4309..4401
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          4327..4331
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4340..4346
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4367..4371
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4381..4386
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4394..4397
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4441..4532
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4458..4462
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4473..4477
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4498..4502
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4512..4517
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4525..4528
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4579..4663
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          4597..4601
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4612..4616
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4637..4641
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4651..4656
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4716..4807
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          4738..4741
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4749..4753
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4774..4778
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4788..4793
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4817..4908
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4835..4839
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4850..4854
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4875..4879
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4889..4894
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4902..4905
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4952..5043
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4970..4974
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4985..4989
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5007..5011
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5024..5029
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5037..5040
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5090..5181
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5123..5127
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5148..5152
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5162..5167
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5229..5320
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5246..5250
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5261..5265
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5286..5290
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5300..5305
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5313..5316
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5366..5458
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5384..5388
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5399..5403
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5421..5425
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5438..5443
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5451..5454
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5500..5590
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5518..5522
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5533..5537
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5559..5562
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5572..5577
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5585..5588
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5638..5726
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5655..5659
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5668..5672
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5692..5696
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5706..5711
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5719..5722
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5917..6007
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5934..5938
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5947..5951
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5972..5976
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5986..5991
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5999..6002
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6019..6109
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6036..6040
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6049..6053
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6074..6078
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6089..6094
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6102..6105
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6131..6220
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6148..6152
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6161..6165
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6186..6190
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6200..6205
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6213..6216
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6234..6318
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6246..6250
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6259..6263
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6284..6288
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6298..6303
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6311..6314
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6338..6434
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6355..6359
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6368..6372
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6399..6403
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6413..6418
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6441..6532
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6458..6462
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6471..6475
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6498..6502
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6512..6517
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6525..6528
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6548..6637
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6565..6569
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6578..6582
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6603..6607
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6617..6622
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6630..6633
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6675..6765
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6692..6696
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6706..6710
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6731..6735
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6745..6750
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6758..6761
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6778..6868
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6795..6799
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6808..6812
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6834..6838
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6848..6853
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6861..6864
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6989..7078
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7006..7010
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7019..7023
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7045..7049
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7059..7064
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7072..7075
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7107..7198
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7125..7129
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7138..7142
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7165..7169
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7179..7184
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7192..7195
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <7302..7471
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          7471..7560
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7488..7492
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7501..7505
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7527..7531
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7541..7546
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7554..7557
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7589..7683
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7607..7611
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7620..7624
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7649..7653
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7663..7668
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          <7816..8385
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <8518..>8740
                     /region_name="wall_bind_EntB"
                     /note="cell wall-binding protein EntB; NF040676"
                     /db_xref="CDD:468642"
     Region          <8848..9093
                     /region_name="wall_bind_EntB"
                     /note="cell wall-binding protein EntB; NF040676"
                     /db_xref="CDD:468642"
     Region          <13588..13805
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          <14931..15633
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <15744..16255
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          16404..16494
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          16421..16425
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          16434..16438
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          16460..16464
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          16474..16479
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          16487..16490
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          16524..16621
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          16542..16546
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          16555..16559
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          16587..16591
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          16601..16606
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          16614..16617
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          16632..16722
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          16649..16653
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          16662..16666
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          16688..16692
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          16702..16707
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          16715..16718
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          16728..16814
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          16744..16748
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          16756..16760
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          16784..16788
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          16798..16803
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          16807..16810
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          16819..16903
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          16836..16840
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          16848..16852
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          16873..16877
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          16887..16892
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          16896..16899
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          16924..16993
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          16926..16930
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          16938..16942
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          16963..16967
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          16977..16982
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          16986..16989
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          16999..17082
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          17015..17019
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          17027..17031
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          17052..17056
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          17066..17071
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          17075..17078
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          17098..17174
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          17103..17107
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          17116..17120
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          17142..17146
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          17156..17161
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          17169..17172
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          17180..17267
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(17180,17246,17261)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(17262..17263,17265..17266)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          17282..17371
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          17299..17303
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          17312..17316
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          17337..17341
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          17351..17356
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          17378..17466
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          17394..17398
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          17407..17411
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          17432..17436
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          17446..17451
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          17459..17462
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <17482..17912
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     CDS             1..17949
                     /gene="sls"
                     /coded_by="XM_077645939.1:124..53973"
                     /db_xref="GeneID:144113047"
ORIGIN      
        1 massasttti ssiavqsgnt rlviallqsg dalelriqel spemsdvgsn yeealhlqqq
       61 hqqvlqklqn kqspveells qadelianqk pkaqvysama hnlglawknl naqlehrsiv
      121 ldtavhyhaa akklsdelak geqeftdtfl pdqvaacear lqklnearka ileaskrslq
      181 pfqnllqtlk evastgdsrp lqpaaalsai aalegqhesl qdqrrklgll cearkealek
      241 rlralllfre ldeverqleq spedtalgds passeillhe httriasane klrdrmlkll
      301 ksakqiqdae vearafdlma rvtsrierde arkkdlkeav effneaqtal nkldqlqiqv
      361 sqgpvqdlaa atvlldacie rplllghgil arlsvgtsgv qdkvdelqsr hlhvrqlyeq
      421 kaadvvesse rainfltqcn tlykwcievv qtfleqsshl stgaaaahdf lqshqrilne
      481 mqarepqltm lltqddsrsk ellqlwqqlr slleqrirla qryltcckis tqliedlkti
      541 sdagperqas inenfmqfdh nannflhdaq riqdtwldvt kavrhiqetl dqcyqlknnp
      601 ginseqlikd aqktmesavk ieselypaip relnraetia qylgrhldtl mplvrtiqse
      661 ldarieatsm lqrrdhlfqq lrstqqsfqk nisqyqelvk amisffdyfa qlekklqdth
      721 evegllaeak qraeklatlv dktepseaav ldrdkihslv dnqgallvas lrksqqdsqm
      781 skfneelqli nqqvreltds lkrdsskchv vefqqriqsl errievlcts srlqtesarv
      841 eleeltsrwr nfhkeamaap ppqpverktq evkevcgpsf vvplddvevq egakcslvcv
      901 laghppptvq wykdgvsirg nsdyvttsqq ledgtseqkl tieetladds akftckaina
      961 aglaetscrl svreqprena leevfieale eqhtthttgg vlteptfiep lpataqlrpg
     1021 eelhlectvq grpeprvsws kdllpvrdaa kpsqeqgmar lvlsgatesd sgtytavakn
     1081 kagetacscq vkvaedaapp epprvlkale dlevkpgpdp itleciivgr pepeviwyhn
     1141 tqpikeserv rllfrgdkcs lvlngvsaqn agtyrcsavn pmgscytecn mrvplsapvf
     1201 leplrdvttd egcrvvltak lwapeppfvr wfkdgrevlp spdfqvshdp dgtvkllipk
     1261 aaasnsghye veasnpggrt rtgckvhvre aqkavqassq lavsrtsqqt vsvsksgsel
     1321 rlarslpsel vvqsgtkvcl svacsqpagg qltaswfrsg qpiadspdfr ltrslenvgg
     1381 aslsvfslti seafpedsgd levrvqgpsg vvtartklvv ldedddvtds vqsrdesiqq
     1441 arevkvyetk larpgpvdsa glaltgdlql rvtevthvne mppevseekt tkvvdqagak
     1501 dkvteevtrr vtkrvteitk thtkelstsr asraagplgr vepipvapep kfdilepkyd
     1561 vplfgsrpir apspppappe esrpslfepl svrtsfclet gqfqsprsii rtktaspvws
     1621 pkkkavviae npatgqrvsa pvflqgpedv laaqgqpatl ralvtgcpap rlrwfkdgqp
     1681 vhgpdhvvgq psgpapwtaw ltipeafped sgvyvctasn lvgntsaccr ltvfetgmvv
     1741 qpphtwgppn aspptfepit qgggllyegs dakfqvkvhg npppevvfsr rgmplrndpr
     1801 rqvtydpttg icclhirnlt aeddgdyncv avncageasl tltiraaaaa vmrgqmqqmt
     1861 tvqrtqvvds rthvqdgvsp ihaqpihyfa ghdqtfrvdt feyrllreve frqfvigrea
     1921 apqpaipgap isapqiqarp rnskllegsd atftvkiasn ppaqvywfrn gellqasqrv
     1981 imeqqgstyi lrvhmalped agyytvlaen sqgrvacsah lviesmpkae eprqadsssf
     2041 vmtetsrtlk psfvkipqdq evtegkmvrf dlrvsgrpfp ditwlrngqp vnddathkll
     2101 vneggqhalm itaasredag twtciannks gevrfevhlv viekeqvvap kfverfqsln
     2161 vregesvtlh cravatpapr itwqkdgrqv hsqppnliie tqdgssalyl nrasvadsaw
     2221 yqctaqnqag statrarlhv iaepkkhtep walhlpkptk vispeksppr etiwlrhver
     2281 ayqpvrgqdd erapqkpaft thlqdlvlhe gerghldarl ipigdetmti ewfcngrpie
     2341 assrvmttyr fgyvaltllh vypedsgvyv craaneagea tttatlrcta rpaierkaiq
     2401 edsiraiqel edaekysrql sidetasltk pvfirplsnk dnlleganch feaqltpvnd
     2461 stmkvewfln gqplttgsri nstfsfgyva lnimslrced sgvymckatn akgeavstat
     2521 lrvkaqglvt gdlgipeqea yirktqelea yqmslhapke iifepvstmk pnfktglrdq
     2581 lnvkenstvh fearleptgd ssmrvewfkd grpleagsrt ttffnfgyvs ltirgvdsrd
     2641 sgvytcqatn sigsattqar lvcvsqasiv ketqhpgglq kiqhledsah yqrseleevs
     2701 ikekpkftqp lqgpsaikeg qsvhletrle pmgdanmkve wfhngrplat ghrfktyfdf
     2761 gyvaldilyt fpedtgtfev ratnklgtdv ltkeitvqak asvdtsaihe vglekieyle
     2821 gsgvdhssyf hieevtrtkp yfkiplkdpk gplkegdsiv lecaieplqd ptmkvewffn
     2881 grplptghrf itkydfgraf lqilnlvped tgqytlratn hlgsahstsc ikvigksgvi
     2941 tesqypegwe kiqhledhsr yarqehvesv itqkptfsrp lhnvetvega nvhlecrlqp
     3001 vgdptmrvew frnsvpikvg hrfrpahefd yvaldilsfy pedsgiytck atsslgenvt
     3061 scnvncfaks qlilesqhpe glqkiqqled qsryrreiie ettvktkpsf tsnmtalslr
     3121 egqnahlecr lepvndadlr vewfrngvsl pighryrpfh dfgyvalnil slvpedsgty
     3181 tvratnslgk aelsttinve gkssidtdtq hpeglqkiqa leghhyerdt ddldqsvtta
     3241 pvftsapksi vvqegqkahl ecrlipvgdt klkvewfkng qpvpagsrfv emcnfgfvsl
     3301 dilntyaeds gtytckatnq lgeavvsaql kchaekslil dtqnqeayek iqqledygrq
     3361 arpayvveei ttqapvftqa mknlslnenq sahfecklip vgdpnlkvew fhnglpiqka
     3421 nrvntihdfg fvaldlsyvk aqdsgtytck atnslgsavc satlnvqdsk slvfdtqhpe
     3481 glqkiqqlee lgrykpevtq eapcpgppmf vtqlqgpsrl tegesahlec riapypdatm
     3541 kvqwfhngve lqsghryrtm ydfgfcaldi lsanaehsge yvvqatnelg tarstarihv
     3601 dakggviles qqpdalpkik qleescgyir ptqeevvikd kpnfvrglyn letlhegqsa
     3661 hleatltpin danmkiqwfh ngveiplghr fktvsdfgyv alnilyaype dsgtymckat
     3721 nqlgeavttc sinvlgksai vtdtyhekgl ekirqleeyq apekpeqviq lqrpvftvpl
     3781 nsldglvegq sahlecrlep indadlkvqw yvngveirpg hrfrtthdfg yvaldimyvy
     3841 aedsgtymck atnslgeavt tcnlralpkq kifydthhpe glekirelea qvkyqsaeiq
     3901 ekpiskpvfi telrgtqeis egesahlecr vepahdaklk idilhngrpl taatrvhits
     3961 dfgyvaidat saipedsgty tvratndlgt aettatlrvl pkssiisdtq hpeglakire
     4021 medesrfkre viqepvtyqt pvftvplqnl enlvedqrnv hlecrlipvg dptlnvqwff
     4081 ndtplmegtr fhpvhdfgyv aldmdyvrpe dtgvytckat nslgqavttc mlkvkpkasi
     4141 lldtlqpqgy ekireledlk gqkppekpda vyekpvftsh lvgpgeineg qparlecrcv
     4201 pvgdpdlkfy wyvngielpk gsrlipnndf gfvtldilsg iaedsgvymc kavnkageav
     4261 tstslrvkgr agvlldshhp eayrqtqkfe ydssripekw sdekpkaapv fvqhlnnidg
     4321 aveghylrie grieptnddk lkvkwfkngk plvmgtrikp tddfglvsld issarpddsg
     4381 iytckatndv geaistctik vegreniila sqhpdslpki rqleeyvppe kyvpepdyeg
     4441 pvfvthlnnl eiregatahf ecrvepskdp tlkveflknn kpvpagskyn fnndfgfvtl
     4501 dvsnaypeda giytcrarna kgeavttgsl kvqgksgvls dtlhpmgaqg lskvqelets
     4561 yltryqapve eaekvfprpv fvvplepnfs iqegspvtle ckvepasdpk lrvewflngk
     4621 plapgsrhtl thdfgfvvla mtdfwgrdag vytcrasnaa geafttttit cltrkgvqed
     4681 tlhpegrkgl esiqhleesl trvpeaiqee aagqppvfts qfvnlkdlne geiahfeatl
     4741 tpvgdqtmqv ewffrgkplk aghrirtvha fgmvvleilg tvledsgryt cratnkwgka
     4801 evtvdlectd ktkgqrpqft tqlqnlmdlk egnsahlech lvpvgdpdmk vewyknsqpl
     4861 rdssriktls dfgyvvmdis fvhaedsgdy vcvatnkygs datkctiqca gtgkifrdsl
     4921 qpqsldriae legasaltrt savmeatrlq ppkflsqlsn itnlvegqsa hfecqlvpvn
     4981 dpdltvewyf ngqllrsghr frtfhdfgiv ildilycyge dsgewvckat nklgsdvtra
     5041 tlqckskssl iltpqvppem asatqniial eeslyrtaav iepegpaeap rftvpltnve
     5101 dlregdnahl earltptddp dltvewfknn mplmsgtrir tindfgfvvl emspvypeds
     5161 gvyscrarnr fgeavttctl kcqgkrsiil etqlpesmtt giekiakfee vssaridekw
     5221 tdkdtsqppk fittpqdltl aenslahfec rltpvgdptl rvdwyhngkp lvtgsrvkti
     5281 sdfgyvilev agvyprdsgv ytcravnkvg easvscklav kgkqsvvmep qlpqefrsgy
     5341 esiqkleesm yrteekiydd dkkeppkfvt qiqslldkve gdsahfeckl ipvgdpnlkv
     5401 ewflngrplv tgtrvhtidd fgfvvldidw lfprdsgeym cratnrwgsd ttkatlkika
     5461 kkdiimdsql pegmnvdklr dleyptpqee tiqeqepvkp rfitqiqpqq nlnegdsahf
     5521 ecrlepindp klrvewyhng qplrsghrfk tthdfgfval dvlyvypeds gtyvaravnd
     5581 vgedqtqatl rctakpkldy rtqlpkdmkd gvkkiaemea swqraetqee veeepcapmf
     5641 imkpepqvvi egewakfqcr vighpkprli wvlnghtvia gsrykltydg iyhldipktr
     5701 qydqgkvevf arnfcgeayc fttlevrpkf ddyravlkhs pkpwydqdvk syqkyrhete
     5761 lqrvfeeklt pggtridvwk teqgqqgehq kikkrieeee leklkpkver fktdsiyyda
     5821 rtgekkvetg sqaqymakyf eteaekqqrg atgispesvv qgrevhtttq rqtqkeqqgd
     5881 leitrkktlt etleqehkgv tkeqrvqgpa qepakapvft kklqpcrvde grgakfqctf
     5941 tgqpapkitw yrenfpiqps qdfqivttds tstliirevy vedsgvfsvk aenrggsaks
     6001 sanlvveerr eqrsgvvppn ftrtiqdvss kagklvrlda kvsgskpldv ywlkngkkvt
     6061 pdvshkivee ddqytllile aqadsdsgsy ecvainsage arcqahvvie gakpktppts
     6121 pkeapgdqkp ptvteplkpl avkegqsavf rcripavpga qvkwfrgdqq vkqsryfrms
     6181 qennlftlki seafpedegv ykcvatnpag tvstsanlkv ivpelnevpp tvtpladltv
     6241 pegsparfvt slggvpppkv iwvreghiik qsrdfqmnqd qgsaslvirh typedegvyv
     6301 cratnasgqa etsarltvqr ktteqepvra mgpqgrapqi tkqpesrsak pghkvdfkat
     6361 atgsprpdit wykdgrpvst tigdvvvstt ptsqeevttt ltinnvtvdd agkytcvatn
     6421 cwgraesiqv tlevkggkep prfieqlepv aatdgspail rctvvgsqpi hikwfqnrre
     6481 vkdtkdfqmh ydpltglvsl tipeiypddq glytcrafne ygqdetsaav tvidveffgg
     6541 vsdvsvqprf vqplrpmayp egeparlsve vhavpnpdfh wffegkmlks srdflvtseg
     6601 lksslviael fpedagpyec raqnpagrat tsanlrviqe qslptsplsk vqpgaeptee
     6661 rptsppeslt qlkppvftrn lvsqtvvegs pvtfvgevts fpevvsftwy lngkvikssr
     6721 dfqiynegnr ttlkiaevfp ddagtfsvta enpvgiatsk akltveeeai egaameaprf
     6781 lteltpvtvm dggeaklsvy vtgkpmprvt wlhndrevke nsdvwttqrt dgycelfise
     6841 afpedmgeyv ckavniagta ttrthinves yeyvpdseva satydtpgga hthdhtqees
     6901 ydtldtmpsv semdesdmer ipdlprddgr pprmpgdkhk prkshhkrrk kkvsrgstsv
     6961 pkemipvcye pmqpwergrr ktsdpgtapv vvvpledviv plgqpatldc kavgmpkpki
     7021 twyrngvpik hsfeyqvyyd pqgissltia hteledfaey vveaknrygt dttraeliql
     7081 dlnsmrpmrr iqradhrlsl dfksmrprfe vplqsaynkp lgfpvtlect vrgrpkptvh
     7141 wfhngiliep sympvykvti lsdkcmlhit emmpategry vckavneage kttecilhag
     7201 epeelkpeee kpaeekphki paflqskqpr rkpsddeyqs feseselsfk etivpqirkl
     7261 srpkespppe kpektakesa ieflttvlkp twpfsmfrsa spkavptdee qpeperfpsv
     7321 erppsaerpp saerpprppe rfpsaerppr pdrektdrer ppstertrpv rppraepprp
     7381 lrpgehverp pvpeaiqrre ppqpespwhp epqepyqaer pptevdrlap rdagkppigr
     7441 rtpeapermp peefvkwkpg hrrtsepgmp pkfvapledv evlvgypatl dcrtvgvpkp
     7501 kitwykqgqp vkyspeysly ydtegncsit ihrttpedfg dficeaknky gtevteaeli
     7561 qidmksmlpm rktpkkvnkl dlefvpmkpr feqpllsevr aplgmpitle ciargrpppe
     7621 vrwyhedrfi ppddtestvy kvetlhdksl lhivevnett rgryvckatn eagervtecf
     7681 vtigereedk tketlqrtgf evpvstedtv flrplrhekl hikaakeikm peesiverlp
     7741 tmetvdaypq pveatklfiq eevsvkkryi teeitqqeik etkqvtkeqp eadeqipefl
     7801 kkvlkpiprh qqviqeekvd iseitqyetl pkpeeelphp gikeapqqrd eplkhevpld
     7861 kqpkrkvpvv kqedietpef ltktlkptdk frkkdipeal eesefqkltk rrtiqpqqpd
     7921 ykeaeyfeat etmeiikpqk lkkpeekpkd keyvltthet teatdlkfqa rpkpdkpfge
     7981 hpvveeiqvl kkekpteitv esvekpeiad vktvyrpkvm teslerpkei tkpdyktekl
     8041 qeekgfikvf kvpkpkmpgt kekpqeilpt ketediiqlq kklkpeeqke epidergeqg
     8101 lvietikrpk ekkpedkkpq kkpmeqptla emiifkkqeq lpeitteave tphivdvkkl
     8161 lqtdittqas qktkeiaeie ydteevlegr etielmkppk tkkakkkvkp deeepieeet
     8221 dqvidlkska rpkegkpeee kpkkrpqeqp qveeisvikk ketpreitae aietpditdi
     8281 ktvkrtdivt ealqrpdeis qieyetedll eeretielak vpkpkkarrk kpqeeeaqma
     8341 edveqkvhld atarprekkp eekkpvaedi tilkrkespr kitteatelp diidvvttkr
     8401 tdittealer pkeiaqveyt teevveeret ielakmpkpk kpkkkakpee eeipvveete
     8461 qeidlaakar pqkekpekkp eveeitvlkk eesptkitte atelpdiidv attkrtditt
     8521 ealerpkeia qveytteevi eeretielak vpkpkkkakp eeeeipfvee teqeiklask
     8581 trpkkekpev eeitilkkee spreimteat eipeiidvkt vertdittea lerpkeiaqv
     8641 eytteeviee retielakvp kpkkpkkkvk peeeempvve eteqeielas kirpkkekpe
     8701 veeitilkke espreimtea teipeiidvk tvertditte alerpkeitq veytteevve
     8761 eretielakv rkpkkpkkkv kpeeeeipvv eeteqeiela sktrpkkekp eveeitilkk
     8821 eespreitte atempeiidv ktvdrtnitt ealerpkeia qveytteevi eeretielak
     8881 vpkpkkpkkk vkpeeeeipv veeteqeiel asktrpkkek peveeitilk keespreitt
     8941 eaveipeiid vktvertdit tealerpkei aqveypteev ieeketiela kvskpkkpkk
     9001 kpkpeaeeip vaeeteqivd leskarpkee kpeqqpevee itilkkeesp reitteakei
     9061 pdiievktie rtditteale rpkeitqvey ttekvveerq tielakvpkp kkpkkkvkpe
     9121 eeeipvaeet eqivdleska rpkeekpeek peveeitilk keesprkitt eakeipdiid
     9181 vktiertdit tealerpkei tqvdytteev veeretiela kvpkpkkpkk kvkpeeeeip
     9241 vaeeteqivd leskarpkee kpeekpevee itilkkeesp rkitteakei pdiidvktie
     9301 rtditteale rpkeitqvdy ttekvveere tielakvpkp kkpkkkvkpe eeeipvaeet
     9361 eqivdleska rpkeekpeek peveeitilk keesprkitt eakeipdiid vktiertdit
     9421 tealerpkei tqvdytteev veeretiela kvpkpkkpkk kvkpeeeeip vaeeteqivd
     9481 leskarpkee kpeekpevee itilkkeesp reitteakei pdiidvksie rtditteale
     9541 rpkeitqvey tteevveere tielakvpkp kkpkkkvkpe eeempvmeet eqvidleska
     9601 rpkeekpeek peveeitilk keespreitt eakeipdiid vksiertdit tealerpkei
     9661 tqveytteea veeretiela kvpkakkpkk kvkpeeeemp vmeeteqvid leskarpkee
     9721 kpeekpevee itilkkeesp reitteavei pditdvksik rtditteale rpkeitqvey
     9781 rteeaveere tielakvpkp kkpkkkvkpe eegipvaeet eqivdleska rpkeekpeek
     9841 peveeitilk keesprkitt eakeipdiid vktiehtdit tealerpkei tqveytteev
     9901 veeretiela kvpktkkpkk kakleeeeip vveeaeqiid lgskarpkee kpevkeitil
     9961 kkeespreit teakeipdit dvksikrtdi ttealerpke isqveyttee vveeretiel
    10021 akvpkpkkpk kkvkpeeeei pvveeaeqii dleskarpke ekpeekpevk eitilkkees
    10081 prkitteake ipdiidvkti ertditteal erpkeitqve ytteevveer etielakvpk
    10141 akkpkkkakp eeeeipvvee teqvidlesk arpkeekpee kpevdeitil kkeespreit
    10201 teakeipdii dvktiertdi ttealerpke itqveyttee vveeretiel akvpkpkkpk
    10261 kkvkpeeeem pvmeeteqvi dleskarpke ekpeekpeve eitilkkees preitteake
    10321 ipdiidvkti ertditteal erpkeitqve ytteeaveer etielakvpk akkpkkkvkp
    10381 eeeempvved teqiidlttk arpkkekpee kpeveeitil kkeespreit teakeipdii
    10441 dvksiertdi ttealeqpke itqveyttee aveeretiel akvpkpkkpk kkvkpeeeem
    10501 pvmeeteqii dliskarpke empvaeeiti lekefpreit teavelpdir eiktvqrtei
    10561 iteaverpke itevketvee tetidivktr kpkktkkkks pgekevlvte eteemfdves
    10621 karprkkkpg eqegieditv ieeieivkkk pidvttdvte vcegefmqah vseefvvski
    10681 peassaqmsi vasrpiviee isselnadql didkkpkeek pqtaltesqa vtieeaftse
    10741 mpqavkmpeq itreasesvi ieekslvvee ttseqyasef lakapkkrqk akevlepqqs
    10801 vevceagslf ttdelkplsr dvyqktlgil eeqsfairde gkeemvpefa pddvvykeas
    10861 ktllllpqhq vdvtevvpsv sseelviekt aekkgnvtfi eesrefstvl eelpaqfqse
    10921 dvpltsltck iteatrsypq heeqatefhe dlpegikaeq sdveltrgth evhevaaefl
    10981 apsekesvas lsrvdiprev ttveemaaqf dvsgperthl tveftevprd aievqeqvte
    11041 fqpdtvrpll pesknigdik scvdieetat lfeapllkee tlvkdladir ketlvveeiq
    11101 teeqapearl tvipekktvk sskvlplenl pvqvsqvtpd hgtselpsea ptqrhlkhsi
    11161 veelsmtqev atvldeikpe ahveqpmlkd keqvteglti qevtedsiqp eetsscivve
    11221 eeildhaeag ievkkktkkk yvrfddtagt qevtevlhtf srtapegtsl kiedvteqye
    11281 nvpegseqpi ieeaeevqpt peekpeegle ekpkekkkkm vkkkekpevi eiklqpkeea
    11341 peeireiaet reteekvdei tefdiqikkv hkteevteit vtseevpqvd vtqlvqkpve
    11401 lisiekieek peevpevkce erpeevleek pkekkkkkvv kkkeklevte ielkpkdeap
    11461 eeiqeikeik eteekfeeit efdieikkvr kpedvteitv tseevpqvdv mqelekpvel
    11521 isiekieekp eevpeekpee kpeelpeekp kekkkkkvik kkekpevtei elkpkeeapa
    11581 eiqeiteike teekfeeite fdieikkmrk pedvteitvt seevpqvdvm qevekpveli
    11641 siekieekpe evpeevpeek peekpeelpe ekpkekkkkk vlkkkekpev teielrpkqe
    11701 aptemqeite iketeekfee ttefeieikk vhkpedvtei tvtseeisqi dvtqkvekpv
    11761 elisiekiee kpeevpeekp eekpeelpee rpkekkkkvi kkkekpevte ielkpkeeap
    11821 eeiqeiteik eveekfeeit efdikieklr kpeevtditv taeevpqvdv tqhlekpvel
    11881 isiekieekp eempedkpke rpeeiveekp kekkkkkvik kkeklevtql dlkqkeeape
    11941 eiqeiteike aeekfeeite fdieikkvrk peevtditvt seevpqvdvt qvmekpveli
    12001 siekieekpe evpeekpeek peefqeekpk ekkkkkvikk kekaevteie lrpkqeapae
    12061 mqeiteiket eekfeeitef dieikkvrkp edvteitvts qevpqvdvtq evekpvelis
    12121 iektekpeev peekpeekpe elpeekpkek kkkkvikkke kpevtevelk pkeeapegiq
    12181 eiteiketee kfeeitefdi eikkvrkped iteitvtsee vpqvdvmqev ekpvklisie
    12241 kieekpeevp eekpeekpee lpeekpkekk kkkvikkkek pevtelelkp keeapeeiqe
    12301 ikeiketeek feeitefdie ikkvskpedv teitvtseev pqvdvtqkve kpvelisiek
    12361 ieekpeevpe ekpeekpeel peekpkekkk kkvikkkekp evteielkpk eeapegiqei
    12421 teikeveekf eeitefdiki eklrkpeevt ditvtaeevp qvdvtqqlek pvelisieki
    12481 eekpeevpee kseekpeefq ekpkekkkkv ikkkekpevt eielkpkeea pediqeitei
    12541 tetqekfeei tefdieikkv rkpedvteit vtseevpqvd mtqelekpve lisiekieek
    12601 peevpeekpe ekpeelpeek pkekkkkkvi kkkekpevte ielkpkeeap egiqeiteik
    12661 eveekfeeit efdikieklr kpeevtditv taeevpqvdv tqqlekpvel isiekieekp
    12721 eevpeeksee kpeefqekpk ekkkkkvikk kekpevtele ltpkeapkei qeiteikete
    12781 ekleeitefd ieikkvskpe dvteitvtse evpqvdvtqe vekpvklisi ekieekpeev
    12841 peekpeekpe elpqekpkek kkkkvikkke kpevteielk pkeeapeeiq eiteikeiee
    12901 rieevtefdi kigklrkpee vtdikvtaee vpqvdvtqev ekpvelesie kikekpeevp
    12961 eekpeekpee fpeerpkekk kkkvikkekp evteielkpk eapeeiqkia eikeveekte
    13021 evtefdikie klrkpeevte itvtaeevpq advtqlekpv elesiekiee kpeevpeekp
    13081 eekpeefree kpkekkkkvi kkkekpevte ielkpkeape eiqeiteike veekieevte
    13141 fdiiieklrk peevtditvt aeevpqadvt qlekpveles iekieempee kpeekpeefp
    13201 eekpkekkkk kvikkkekpe vtdielkpke eapeeiqeit eikeveekme evtefdikid
    13261 klrkpeevtd itvtaeevpq vdvtqqlekp velesiekie ekpeevpqek peekpeefpe
    13321 ekpkekkkkk vikkkekpev teiemkpkee apeeiqeite ikefeekiee vtefgikivk
    13381 lrkpeevtdi avtaeevpqa dvtqqlekpv elesiekiee kpkevpeekp eekpeevlee
    13441 kpkekkkkkv vkkekpdiaq vemkpmeeap eksevteikp veeieetpkf dveikkphmp
    13501 eevtditvis kevpqadvaq evvkpleies iekieekped kpqerpeevl eekrkekkkk
    13561 kaikkkekpe vteielipke eapdeimtea ieetkvvefg vtvekditds hveliqdaek
    13621 pskidstgif pekpqekdte qpkktkpkhr lsikvlddee dvasptsvdf kfikplseek
    13681 pseepvelvy ethreetidi evtleslpqp tvkeeesklf kkedaleape kpeifeekmp
    13741 eeydestdtr edkvpekqrp kiirpkteqp kgkrkprrrp dtileeeefy sagesiedye
    13801 ekdtemyeha dieleavgid eldvfesavd sselpmlqea lvstlelrpt eeatkempev
    13861 isrpkdelfv tesvagflds vesarvtevq dsslleqava tilvskdvps eeefevsvkq
    13921 deelitlemk lftdtreqeq evqvvfedke etvaplqpti seqpevtprr rrkvpekphk
    13981 eelpatqdiv vsteekpeem pkqeempqev tmeetkpedk apeeaklkik kkpkkskvte
    14041 evpeetvilk kpeekpeelp kevteeimvp leekpeevpk pedvpqkikv evkpeekape
    14101 eaeikikkkp kkpkvkeevp eetvilkkpe ekpeelpkev teeitvplee kpeevpkpee
    14161 vpqqvtveea kpeekvpeee evkikkkpkr pkateevpke tvtlkkheek peeipqevte
    14221 dvtvaleekp evapkpeeap qevtveeakp eekapeeaei kikkkpkkpk vkeevreetv
    14281 ilkkpeekpe elpkevteei tvpleekpee apkpkevpqe vtkeeakpee kapeeaeiki
    14341 kkkpkkpkvt eevpeetvii kkpeekpeel pkevteeitv pleekpeeap kpeevpqeit
    14401 veeakpeeka peeaeikikk kpkkpkvtee vpketviikk peekpeelpk evtgevtvpl
    14461 eekpeeapkq eevpqevtve eakpeekape eaeikikkkp kkpkvkeevp eetvilkkpe
    14521 ekpeelpkev teeitvplee kpeeapkqee vpqevtveea kpeekapeea eikikkkpkk
    14581 pkvteevpke tvilkkpeek peelpkevte eitvpleekp eevpkpeevp qevtveeakp
    14641 kekapeeaei kikkkpkkpk vteevpeetv ilkkpeekpe elpkevtkei avpleekpee
    14701 vpkpeevpqe vtveevkpee kapeeaeiki kkkpkkpkvt eevpeetvii kkpeekpeel
    14761 pkevtgevtv pleekpeeap kpeevpqevt veeakpeeka peeaeikikk kpkkpkvkee
    14821 vreetvilkk peekpeelpk evteeitvpl eekpeeapkp kevpqevtke eakpeekape
    14881 eaeikikkkp kkpkvteevp eetvilkkpe ekpeelpkev aeeitvplee kpeevpkpee
    14941 vpqevtvkea kpeekvpeea evkikkkpkk pkakeevpee tvtlkkheek peeilqevte
    15001 dvtvvleekp papmpevape evtveekpkt edkremfeee aklvikkkpk kpklkeeape
    15061 etvtlkkpee kpedrpeeis editvsleek pkeapkpeev sdevtveekl kpedkkevae
    15121 eeakltikkk pkkpkvkeea peetatlkkp eekpeerpee iteditvtle ekpkevpkpe
    15181 evseeltvee kpkpeikrev veeeakltik kkpkkpklke evpeetvtlk kpeekpeerp
    15241 eeiseditvt leekpkeapk peevsekltv eekpkpedek evveeeaklt ikkkpkkpkl
    15301 keeapeetvt lkkpeekpee rpeevnedit vsleekpkea pkpeevseev tveekpkpev
    15361 mrevveeeak ltikkkpkkp klkeeapeet vtlrkpeeks eerpeeised itvtleekpk
    15421 eapkpeevpe eltveekptp evkkevveee akltikkkpk kpkleeapee tvtlkkpeek
    15481 peerpeeise ditvtleekp keapkpdevs eeltveekpk peakrevvee eakltikkkp
    15541 kkpkleeape etvtlkkpee kpeerpeeis editvtleek pkevpkpeev seeltveekp
    15601 kpeikrevve eeakltikkk pkkpklkeev peetvtlkkp eekpeerpee iseditvtle
    15661 ekpkeapkpe evseeltvee kpkpevkrev veeeakltik kkpkkpklke eapeetvtlr
    15721 kpeekpeerp eeiseditvt leekpkeapk peevpeeltv eekptpevkr evieeeaklt
    15781 ikkkpkkpkl eeapeetvtl kkpeekpeer peeiseditv tleekpkeap kpeevseelt
    15841 veekpkpevk revveeeakl tikkkpkkpk lkeeapeetv tlrkpeekpe erpeeisedi
    15901 tvtleekpke apkpeevsee ltveekpkpe akrevveeea kltikkkpkk pklkeevpee
    15961 tvtlkkpeek peerpeeise ditvtleekp keapkpeevs eeltveekpk peakrevvee
    16021 eakltikkkp kkpkleeape etvtlkkpee kpeerpeeis editvtleek pkevpkpeev
    16081 seeltveekp kpeikrevve eeakltikkk pkkpklkeev peetvtlkkp eekpeerpee
    16141 iseaitvtle ekpreapkpe evseeltvqe kpkpiqkevv eeeakltirk pkkredfeev
    16201 teetptvtlk kraskadald dvsqkftlpl rkdsaaprqy deveemfelk vprkistddt
    16261 givlkpkdee qteqedtlll dltskekeqv vseasdefki vlqqpqgeee eieiveyvai
    16321 ttyiteeedg ldleegehvi lverkseeiw lvrktitkek glvpksilqd vasynrlldq
    16381 vleekiskip afgklrpgek mtapkflkkp eptevekgqp atfkctvqgn prpyvtwfkq
    16441 triiksttef eiyydednvs tlvinevfpe dagtytvvak npagfascsa dltvqefise
    16501 qtisrrtvsr ssslldilve gippvfsapl rphlrvdegs tltlvikvtg tpepdvtlkv
    16561 ndkplnekdd krfsvvktqe dhnfinvtfv lkdvkpsdeg tyevmaense gsvtshidve
    16621 viekpkeeqe ppkftqtfsd tvvteeetvr livkytgkpp ptvlwyrndk pipkdstkii
    16681 tredeststl eipksevadt asytctasnp agkvthtakv tvqepnlefv eklqdievke
    16741 nhvamfvtkt nrpvdhvtwc kdgepiklde phdkyqaise gpyqklvikn ssikdegeyt
    16801 cvlgdqetsa dlvvielppe ittfmtdqtv akddtaqffi eltkgdalvr wfkdgkelqf
    16861 sdrvrlkidg krqvliikns vrqdagrytc kvgdqscsar ltveppsvef tarlpekttv
    16921 pqdtdaifev alsradvdvv wmkngkpipm dqrfkfvkee kvrrlvvpcv dkedtaeytc
    16981 vagnvktsti lhvedadaef tlrlhdvivr esdtvtllve vsretlevrw mkdgdtihps
    17041 ekvlvttegk srkliiress iddqgvyscv lkdkkcsssv vvhappkitt skenfstrkg
    17101 ttlklpipyt gipqptatwk lngqplepvk nkiiqeispq eatltikdlq qadtgtvtlt
    17161 lknscgecsk pftvtcietp sapgtpepsd isssgsltls wkppksdggs kitnyiieyh
    17221 drntlrwily sdtvqdtftk veglspdeey mfrvtaenea gkgdpspgtk yiriqpkage
    17281 ppvilehltd kvvgikkpae ltcrisgnpt ptvkwlkngk dlvmisnfsa efkdqkavlr
    17341 ikeastksgg kytckatnph gstettctls vqdppraefd eklrnvrink qdefkvpcti
    17401 hgfpkptvaw fhdgvplqst khtlitekdd tstitihsae gpdsgiytie lkntagsisy
    17461 dfklrvldkp gppqepiqfl etagdhitls wqpplddggs pltkyiiehc ettrtvfiev
    17521 gttqpdtpsf kdpdvtegne ylyrisavne ygtsapltse pvvarcpydk psapegpltt
    17581 akmtdtslvl twkeplsdgg rpiidytiek reaskkawik vgtteslsme vtnlksdnay
    17641 mfrvtcrnei gssppltsee pitvhaqiqp pdaptgplri sdvtnktatl twsppafdgg
    17701 spivayiveh lsedqaavdp pkwatvanvd ggttthrvis lsdktrhrfr vcaenvagvg
    17761 ppledtgewq tlpaaadrpt pptapleilv tgpnsmdvsw grpewdggsp llgytaalrd
    17821 tsrtmwmevg qvdaetlrlh vkdleegaqy hvrilarndv gfsdplesee pvtilrppgf
    17881 qdqattspek kpkakaeets edsyttqtts swireanvep llhsytkhvl vsrneyffrl
    17941 whyadtlfk
//
LOCUS       XP_077502066            1084 aa            linear   INV 25-NOV-2025
DEFINITION  dynactin subunit 1 [Amblyomma americanum].
ACCESSION   XP_077502066
VERSION     XP_077502066.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645940.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1084
                     /product="dynactin subunit 1"
                     /calculated_mol_wt=121810
     Region          11..72
                     /region_name="CAP_GLY"
                     /note="CAP-Gly domain; pfam01302"
                     /db_xref="CDD:460154"
     Region          <121..>529
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          442..676
                     /region_name="Dynactin"
                     /note="Dynein associated protein; pfam12455"
                     /db_xref="CDD:463591"
     CDS             1..1084
                     /gene="DCTN1-p150"
                     /coded_by="XM_077645940.1:230..3484"
                     /db_xref="GeneID:144113048"
ORIGIN      
        1 mttydgrpie vgarvevlgk dvrgrvafvg stsfsmgrwv gvvldepkgk nngtvqgrty
       61 fscadnhgif vrqlqlrlld depaaeapaq akplatttpg ptvepvaldq peplsrtwvv
      121 eeasaekqaa qkeqdaekqt lkaqildlqe kldtlmqkrq eskaqlrdye rtrlqlqqll
      181 efkskisesq aelqkqlaqa rreaqeaqde rerhaeevsd mveamematv ekemaeekle
      241 qlqqevdhwk ekyemleldy ktltselstg epssykerql aqenekmrea lmrlrdlmtq
      301 lknekqqqkk eldrsraeaq dlarmkqlle erhqqaecsi telqeqldma lgaeemvehl
      361 teknldleek lrdlreavdd leklhdlnee llqqaretel elreelaqah vrladsqrrv
      421 eaahealadc eqtlakyhal vaqlreqaqg leqqlqsept pvaveasela lrlaqgkaha
      481 ravdlelrrl evaqsnrhvd yltaflsegf lahehegvlt lllvarlgak cgilakqleq
      541 rwagavpgpg qpveavqysh aclvrfllgr lqgllhqyat aldscsvelf aktatlypem
      601 lgherlvdly vqllrkdeld envslgnler alsyfqslya vhlsnerqde rhlltqslrc
      661 lalaceavqc algfvrhvlg aqggdlaall daalssasdv vqavrkvrrr lppdegpvtl
      721 gaeareqlea evrslgqvvq aacalqrava srieheplpp ldklkelahe atdrvygked
      781 qgpdclreal aaavstlerl vdavqpqepq pkhvapiqlr aqaarqqard leavrsslea
      841 rendiialkk alkikseecs emhvrkdmae kklevltrds dervdkvqrq ldetkmrlrk
      901 kekefeetmd hlqadidale tergelkdkl kmiskktlyk glsetvglpa stswpqlqdi
      961 kvalrhvhse rakleshrvs qllaslppla pvppraeqqr dacakaaraq sqalalcvqv
     1021 rvvdlsnrra pvqgpnlaal iearqcleas rhelvllral avarvaaawr afpnpalvrl
     1081 lqaq
//
LOCUS       XP_077502067            1084 aa            linear   INV 25-NOV-2025
DEFINITION  dynactin subunit 1 [Amblyomma americanum].
ACCESSION   XP_077502067
VERSION     XP_077502067.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645941.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1084
                     /product="dynactin subunit 1"
                     /calculated_mol_wt=121810
     Region          11..72
                     /region_name="CAP_GLY"
                     /note="CAP-Gly domain; pfam01302"
                     /db_xref="CDD:460154"
     Region          <121..>529
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          442..676
                     /region_name="Dynactin"
                     /note="Dynein associated protein; pfam12455"
                     /db_xref="CDD:463591"
     CDS             1..1084
                     /gene="DCTN1-p150"
                     /coded_by="XM_077645941.1:298..3552"
                     /db_xref="GeneID:144113048"
ORIGIN      
        1 mttydgrpie vgarvevlgk dvrgrvafvg stsfsmgrwv gvvldepkgk nngtvqgrty
       61 fscadnhgif vrqlqlrlld depaaeapaq akplatttpg ptvepvaldq peplsrtwvv
      121 eeasaekqaa qkeqdaekqt lkaqildlqe kldtlmqkrq eskaqlrdye rtrlqlqqll
      181 efkskisesq aelqkqlaqa rreaqeaqde rerhaeevsd mveamematv ekemaeekle
      241 qlqqevdhwk ekyemleldy ktltselstg epssykerql aqenekmrea lmrlrdlmtq
      301 lknekqqqkk eldrsraeaq dlarmkqlle erhqqaecsi telqeqldma lgaeemvehl
      361 teknldleek lrdlreavdd leklhdlnee llqqaretel elreelaqah vrladsqrrv
      421 eaahealadc eqtlakyhal vaqlreqaqg leqqlqsept pvaveasela lrlaqgkaha
      481 ravdlelrrl evaqsnrhvd yltaflsegf lahehegvlt lllvarlgak cgilakqleq
      541 rwagavpgpg qpveavqysh aclvrfllgr lqgllhqyat aldscsvelf aktatlypem
      601 lgherlvdly vqllrkdeld envslgnler alsyfqslya vhlsnerqde rhlltqslrc
      661 lalaceavqc algfvrhvlg aqggdlaall daalssasdv vqavrkvrrr lppdegpvtl
      721 gaeareqlea evrslgqvvq aacalqrava srieheplpp ldklkelahe atdrvygked
      781 qgpdclreal aaavstlerl vdavqpqepq pkhvapiqlr aqaarqqard leavrsslea
      841 rendiialkk alkikseecs emhvrkdmae kklevltrds dervdkvqrq ldetkmrlrk
      901 kekefeetmd hlqadidale tergelkdkl kmiskktlyk glsetvglpa stswpqlqdi
      961 kvalrhvhse rakleshrvs qllaslppla pvppraeqqr dacakaaraq sqalalcvqv
     1021 rvvdlsnrra pvqgpnlaal iearqcleas rhelvllral avarvaaawr afpnpalvrl
     1081 lqaq
//
LOCUS       XP_077502068            1084 aa            linear   INV 25-NOV-2025
DEFINITION  dynactin subunit 1 [Amblyomma americanum].
ACCESSION   XP_077502068
VERSION     XP_077502068.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645942.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1084
                     /product="dynactin subunit 1"
                     /calculated_mol_wt=121810
     Region          11..72
                     /region_name="CAP_GLY"
                     /note="CAP-Gly domain; pfam01302"
                     /db_xref="CDD:460154"
     Region          <121..>529
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          442..676
                     /region_name="Dynactin"
                     /note="Dynein associated protein; pfam12455"
                     /db_xref="CDD:463591"
     CDS             1..1084
                     /gene="DCTN1-p150"
                     /coded_by="XM_077645942.1:148..3402"
                     /db_xref="GeneID:144113048"
ORIGIN      
        1 mttydgrpie vgarvevlgk dvrgrvafvg stsfsmgrwv gvvldepkgk nngtvqgrty
       61 fscadnhgif vrqlqlrlld depaaeapaq akplatttpg ptvepvaldq peplsrtwvv
      121 eeasaekqaa qkeqdaekqt lkaqildlqe kldtlmqkrq eskaqlrdye rtrlqlqqll
      181 efkskisesq aelqkqlaqa rreaqeaqde rerhaeevsd mveamematv ekemaeekle
      241 qlqqevdhwk ekyemleldy ktltselstg epssykerql aqenekmrea lmrlrdlmtq
      301 lknekqqqkk eldrsraeaq dlarmkqlle erhqqaecsi telqeqldma lgaeemvehl
      361 teknldleek lrdlreavdd leklhdlnee llqqaretel elreelaqah vrladsqrrv
      421 eaahealadc eqtlakyhal vaqlreqaqg leqqlqsept pvaveasela lrlaqgkaha
      481 ravdlelrrl evaqsnrhvd yltaflsegf lahehegvlt lllvarlgak cgilakqleq
      541 rwagavpgpg qpveavqysh aclvrfllgr lqgllhqyat aldscsvelf aktatlypem
      601 lgherlvdly vqllrkdeld envslgnler alsyfqslya vhlsnerqde rhlltqslrc
      661 lalaceavqc algfvrhvlg aqggdlaall daalssasdv vqavrkvrrr lppdegpvtl
      721 gaeareqlea evrslgqvvq aacalqrava srieheplpp ldklkelahe atdrvygked
      781 qgpdclreal aaavstlerl vdavqpqepq pkhvapiqlr aqaarqqard leavrsslea
      841 rendiialkk alkikseecs emhvrkdmae kklevltrds dervdkvqrq ldetkmrlrk
      901 kekefeetmd hlqadidale tergelkdkl kmiskktlyk glsetvglpa stswpqlqdi
      961 kvalrhvhse rakleshrvs qllaslppla pvppraeqqr dacakaaraq sqalalcvqv
     1021 rvvdlsnrra pvqgpnlaal iearqcleas rhelvllral avarvaaawr afpnpalvrl
     1081 lqaq
//
LOCUS       XP_077502069             962 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113050 [Amblyomma americanum].
ACCESSION   XP_077502069
VERSION     XP_077502069.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645943.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..962
                     /product="uncharacterized protein LOC144113050"
                     /calculated_mol_wt=104859
     Region          386..519
                     /region_name="Sod_Cu"
                     /note="Copper/zinc superoxide dismutase (SODC); pfam00080"
                     /db_xref="CDD:459663"
     Region          <735..822
                     /region_name="Sod_Cu"
                     /note="Copper/zinc superoxide dismutase (SODC); pfam00080"
                     /db_xref="CDD:459663"
     CDS             1..962
                     /gene="LOC144113050"
                     /coded_by="XM_077645943.1:143..3031"
                     /db_xref="GeneID:144113050"
ORIGIN      
        1 mlllllllml ctgsarglha pfnmagvqge irlvpaapsg vnitvrlvgp pgefswsvre
       61 mpalfdasap cadlgrarhd lsrrhgplel ngtelsfedd qlelqgagsv wgralllqqg
      121 ktqacanllh eegplhwaea lfagprvagr vlfleapsas lvavllfqgg rssryawqll
      181 eadvldaapg crflqmvydp dradgegcsr aahercragd lsakhgplnv gsprgsrrav
      241 ldthlplghr rlflalreea tmafaacapl rvleartvha klprggqlsf tqqspvqptl
      301 vraqgllgas falhpapyce gplrgspavq lfapvslepq lslfgprriv grwisvsrge
      361 dspaecarig fpgptvvgka tfhypvsghv flrqdandqq setavlikmd pygfndttqy
      421 klqvherrpg rdfynwtarc vsagdvfdpl kvgsgpcphp llcpvgalta kgakptfpky
      481 ffytdlhlpl sgphsvlqrs lvlhdsgapp hrgdrlacas llplhpmsaa vrrwlpgeqg
      541 vlghmawvqd spedsvrvev rlrglggras ayrvhalpvp leqrfpckav rdaqgaavel
      601 ssrygdlagk gdeslkalda elqlfgnasv vgksvviqmg errsvcgtil pemeprqgre
      661 lvaiatfdhp esalhgyvrl rqleykdggt sdtfilvdlr hpgkynrnqt kghhwaayvn
      721 qvahdaleks ekarciaagy rwnpylaqsd kdsyhtecsp rtplrcemgd lsgklgplsi
      781 gtgpaiytdt nlplvgnfsv lgrsiivfak dgsmlrkaca nikldihlvr hvsvrkfpgf
      841 ssgafmdhmr tllnasdwlv msdfqaeqdi legqctqltv hffgpeahrl qiefgnlitl
      901 gsvrrqtrtg lkliktfyrp cktldeelsd hsvrvavglp lllllpllpp vdslgrrvel
      961 gp
//
LOCUS       XP_077502070             777 aa            linear   INV 25-NOV-2025
DEFINITION  nuclear receptor binding SET domain protein [Amblyomma americanum].
ACCESSION   XP_077502070
VERSION     XP_077502070.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645944.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..777
                     /product="nuclear receptor binding SET domain protein"
                     /calculated_mol_wt=86967
     Region          91..189
                     /region_name="PWWP_NSD_rpt1"
                     /note="first PWWP domain found in nuclear receptor-binding
                     SET domain-containing (NSD) proteins; cd20144"
                     /db_xref="CDD:438972"
     Site            order(102,105,133)
                     /site_type="other"
                     /note="putative methyl-histone binding site [polypeptide
                     binding]"
                     /db_xref="CDD:438972"
     Site            103..106
                     /site_type="other"
                     /note="PWWP sequence motif"
                     /db_xref="CDD:438972"
     Region          234..275
                     /region_name="PHD1_NSD"
                     /note="PHD finger 1 found in nuclear receptor-binding SET
                     domain-containing (NSD) proteins; cd15564"
                     /db_xref="CDD:277039"
     Site            order(234,243..247,252,269)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277039"
     Region          281..324
                     /region_name="PHD2_NSD"
                     /note="PHD finger 2 found in nuclear receptor-binding SET
                     domain-containing (NSD) proteins; cd15565"
                     /db_xref="CDD:277040"
     Site            order(289..293,299,319)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277040"
     Region          325..375
                     /region_name="PHD3_NSD"
                     /note="PHD finger 3 found in nuclear receptor-binding SET
                     domain-containing (NSD) proteins; cd15566"
                     /db_xref="CDD:277041"
     Site            order(325,342..346,350,370)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277041"
     Region          389..429
                     /region_name="PHD4_NSD"
                     /note="PHD finger 4 found in nuclear receptor-binding SET
                     domain-containing (NSD) proteins; cd15567"
                     /db_xref="CDD:277042"
     Site            order(389,398..402,406,424)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277042"
     Region          437..532
                     /region_name="PWWP_NSD_rpt2"
                     /note="second PWWP domain found in nuclear
                     receptor-binding SET domain-containing (NSD) proteins;
                     cd05838"
                     /db_xref="CDD:438963"
     Site            order(448,451,480)
                     /site_type="other"
                     /note="putative methyl-histone binding site [polypeptide
                     binding]"
                     /db_xref="CDD:438963"
     Site            449..452
                     /site_type="other"
                     /note="PWWP sequence motif"
                     /db_xref="CDD:438963"
     Region          564..610
                     /region_name="AWS"
                     /note="associated with SET domains; smart00570"
                     /db_xref="CDD:197795"
     Region          610..751
                     /region_name="SET_NSD"
                     /note="SET domain (including post-SET domain) found in
                     nuclear SET domain-containing proteins, NSD1, NSD2, NSD3
                     and similar proteins; cd19173"
                     /db_xref="CDD:380950"
     Site            order(621..623,640,650,654,663..669,686..690,697..699,714,
                     725..735,737..741,750)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:380950"
     Site            order(621..623,663..666,686..690,727,734,738..741,750)
                     /site_type="other"
                     /note="SAM binding site"
                     /db_xref="CDD:380950"
     Site            order(640,650,654,665..669,697..699,714,725..729)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:380950"
     Site            order(692,739,741,746)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:380950"
     CDS             1..777
                     /gene="NSD"
                     /coded_by="XM_077645944.1:293..2626"
                     /db_xref="GeneID:144113051"
ORIGIN      
        1 meegmangsd vgeermemeh llnspdgsgp pgfllpggas qrskfgrvrk pkfspcmeyt
       61 plvrshsshn gqkqtevgen skssgtseyl vgdllwskig hhpfwpamvs fdpvsgrymk
      121 vvrknlvlyh vqyfgvqakh gwtqprrvip fegltkfvvl gdlkrppyav plrqrdawma
      181 avdeanaame ltrterkqkl tfeyydsppk parnakavlp lqenssveka kdelclvcek
      241 tgatlactgp ckmsfhlecl giahppavfl cdecttgkhp clvcnktedt ekcsqescgs
      301 fyhvsclkss plplkeeplv cprhfclycf qqkprsfstk grllrcvrcp stfhhgclpa
      361 gssllnssai vcprhsrqhh msaplnvnwc mlcskggrll ccegcpvamh edclglqeap
      421 egaflcpdcl ewkhpkygdi lwvklgcyrw wpaqvcapsd vpaniqalkh epigefavrf
      481 ygshdyywtn krrvflfeeg dggsltassk svarlfeaal eeatlaseqq rkareqsekp
      541 apfrmirsnr pvghtampsv dvykessclc npedpcrtnc lnrlmlyecn rclcpagndc
      601 enqrfqrrqy akasrirspg rgwglqadqs laagdfvmey vgeiideaec errlaqlhme
      661 niqnfyfltl dknriidagp rgnlsrfinh scepncetqk wtvngdtrvg ifaicditpg
      721 teltfnynld crgnekipca cgakncsgyi glrprrpaec epkpkrrkgp rgtgtqd
//
LOCUS       XP_077502071             603 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: ectonucleoside triphosphate diphosphohydrolase
            NTPase [Amblyomma americanum].
ACCESSION   XP_077502071
VERSION     XP_077502071.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645945.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 4 indels
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..603
                     /product="LOW QUALITY PROTEIN: ectonucleoside triphosphate
                     diphosphohydrolase NTPase"
                     /calculated_mol_wt=65595
     Region          162..552
                     /region_name="ASKHA_ATPase-like"
                     /note="ATPase-like domain of the ASKHA (Acetate and Sugar
                     Kinases/Hsc70/Actin) superfamily; cl49607"
                     /db_xref="CDD:483947"
     CDS             1..603
                     /gene="NTPase"
                     /coded_by="XM_077645945.1:18..1829"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 1 base in 1 codon;
                     deleted 3 bases in 3 codons"
                     /db_xref="GeneID:144113052"
ORIGIN      
        1 mrvagllpaq rfvnvgsrlf svsrsavgir dtslqaaerp twrvqlksaq apdqspvtvr
       61 thagatadsl lgafchdrgq eydpalalyt rsneplrgga slralrvrpg dtlyvrhqrs
      121 phlflanwgl lgvlcavlgl lglllvsmay gltggrvpfv hgivvdagss hsqatlyhwe
      181 gakylgtgrv vqmefrdveg gisslgprlt gpalaravsd vradlqppvy lgatagmrll
      241 nltnpmeada ilmavecale pmglrqatil sghdeglfaw lstnylfevi ppasagaetf
      301 gamdlggast qiafavddnv ssndvstlql yghrynvfsv tylcygvnem errylahiva
      361 eqgyartvks pchnsgfnfn rtaeevfesy ctktpatevw lqqhpntvft fvgdgtsagc
      421 kktmtqlmdp rqckhnytdc merpavpvph qikfvgvsaf fytmkglnst xqpltaflni
      481 sdwicsaswd eavktgtpkq ylsryclqsm yirdvlidky gfnedtwtnl afekkangye
      541 lgwslgfmin atnaipaalp stpsiglnlf vllvvlfvll lvlaaiflll alkqsrakln
      601 pps
//
LOCUS       XP_077502072             590 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: TRC8 ring finger protein [Amblyomma
            americanum].
ACCESSION   XP_077502072
VERSION     XP_077502072.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645946.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..590
                     /product="LOW QUALITY PROTEIN: TRC8 ring finger protein"
                     /calculated_mol_wt=64199
     Region          8..515
                     /region_name="TRC8_N"
                     /note="TRC8 N-terminal domain; pfam13705"
                     /db_xref="CDD:463960"
     Region          544..584
                     /region_name="RING-H2_RNF139-like"
                     /note="RING finger, H2 subclass, found in RING finger
                     proteins RNF139, RNF145, and similar proteins; cd16476"
                     /db_xref="CDD:438139"
     CDS             1..590
                     /gene="Trc8"
                     /coded_by="XM_077645946.1:1..1773"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 1 base in 1 codon"
                     /db_xref="GeneID:144113053"
ORIGIN      
        1 msvrgriwaa ldvilrvppl fvmdavlscs lglrydsgav apgvdvikdn sdgsnssgav
       61 hendlfthfd lstllwsfvy lqaflgalvm avlqtrqllh vylwlsslga vlwsyvcnre
      121 lvryaedlsh dgslllellr lepgallrtg anyalqllla lllsagrgpr ataalaltll
      181 aptvasvcll pasplasali aqlllvapal rcappxagsd dvararallg qvggqallea
      241 hwarlraplv lrvfwalrva ahaallpprl plaqalaqla trgcdtsval lgmasvvatl
      301 arltaaaarr llllegsper slatvagllf lvlalqtgit gldpprrlar lvrnlcllat
      361 avlhfvqgml gpllvslgas rsgsrqrhar alglslalaa cpcallaylw thqpistwll
      421 avsafsaelv vkviislliy llfladarre tmweplddyv yylratgsvl eflfgvfllf
      481 ngawifafes rgtirafmmc fhayfniwqq akagwkacar rraalyklhs lpeatsqqlr
      541 elddvcticf qelqtarvtr crhffhsacl rkwlyvrdmc plchstlyhq
//
LOCUS       XP_077502073             565 aa            linear   INV 25-NOV-2025
DEFINITION  G protein-coupled receptor kinase 2 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502073
VERSION     XP_077502073.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645947.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..565
                     /product="G protein-coupled receptor kinase 2 isoform X1"
                     /calculated_mol_wt=64724
     Region          35..178
                     /region_name="RGS"
                     /note="Regulator of G protein signaling (RGS) domain
                     superfamily; cl02565"
                     /db_xref="CDD:470619"
     Region          184..472
                     /region_name="STKc_GRK4_like"
                     /note="Catalytic domain of G protein-coupled Receptor
                     Kinase 4-like Serine/Threonine Kinases; cd05605"
                     /db_xref="CDD:270756"
     Site            order(191..195,199,212,214,246,262..263,265,269,271,311,
                     313,315..316,318,328..329,332,346..351,378,384,387)
                     /site_type="active"
                     /db_xref="CDD:270756"
     Site            order(191..195,199,212,214,263..265,318,329)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270756"
     Site            order(195,269,271,311,313,315,332,346..351,378,384,387)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270756"
     Site            order(328..339,341..351)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270756"
     CDS             1..565
                     /gene="Gprk2"
                     /coded_by="XM_077645947.1:600..2297"
                     /db_xref="GeneID:144113054"
ORIGIN      
        1 melenivant vylkaregga dsnkgkskkw kkilqfphis qcvylrdkvd isygyiveqq
       61 pigrllfrqy cqtkphyckc nsfldaveay elqldedral aaqdvfnkfl seqssefvdv
      121 vnedlvekck qglaeaskdl fsecaravke flmggpfqef egsmyfdryl qwkwlesqpv
      181 taktfrmyrv lgkggfgevc acqvratgkm yackklekkr ikkrrgetmv liekqilqkv
      241 nsrfvvslay ayetkdalcl vltimnggdl kfhmysmgge pgfepqrarf yaaevtqgle
      301 hlhakgivyr dlkpenilld dhghvrisdl glavevpdeq egvrgrvgtv gymapevidn
      361 erytyspdwf slgcllyeml egqapfrark ekvkreever rvkeerekys akfseeakdc
      421 cqqllakspa trlgcqdgra garqvkrhpf fkslnwkrle agmleppfvp dphavyakdv
      481 ldieqfstvk gvnldasdds fysqfnmgsv sipwqtemie tecfkelnvf gpsqtrppdl
      541 cwelppaedr rgcfpfrrka rsrps
//
LOCUS       XP_077502074             562 aa            linear   INV 25-NOV-2025
DEFINITION  G protein-coupled receptor kinase 2 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502074
VERSION     XP_077502074.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645948.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..562
                     /product="G protein-coupled receptor kinase 2 isoform X2"
                     /calculated_mol_wt=64539
     Region          32..175
                     /region_name="RGS"
                     /note="Regulator of G protein signaling (RGS) domain
                     superfamily; cl02565"
                     /db_xref="CDD:470619"
     Region          181..469
                     /region_name="STKc_GRK4_like"
                     /note="Catalytic domain of G protein-coupled Receptor
                     Kinase 4-like Serine/Threonine Kinases; cd05605"
                     /db_xref="CDD:270756"
     Site            order(188..192,196,209,211,243,259..260,262,266,268,308,
                     310,312..313,315,325..326,329,343..348,375,381,384)
                     /site_type="active"
                     /db_xref="CDD:270756"
     Site            order(188..192,196,209,211,260..262,315,326)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270756"
     Site            order(192,266,268,308,310,312,329,343..348,375,381,384)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270756"
     Site            order(325..336,338..348)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270756"
     CDS             1..562
                     /gene="Gprk2"
                     /coded_by="XM_077645948.1:580..2268"
                     /db_xref="GeneID:144113054"
ORIGIN      
        1 melenivant vylkaredsn kgkskkwkki lqfphisqcv ylrdkvdisy gyiveqqpig
       61 rllfrqycqt kphyckcnsf ldaveayelq ldedralaaq dvfnkflseq ssefvdvvne
      121 dlvekckqgl aeaskdlfse caravkeflm ggpfqefegs myfdrylqwk wlesqpvtak
      181 tfrmyrvlgk ggfgevcacq vratgkmyac kklekkrikk rrgetmvlie kqilqkvnsr
      241 fvvslayaye tkdalclvlt imnggdlkfh mysmggepgf epqrarfyaa evtqglehlh
      301 akgivyrdlk penillddhg hvrisdlgla vevpdeqegv rgrvgtvgym apevidnery
      361 tyspdwfslg cllyemlegq apfrarkekv kreeverrvk eerekysakf seeakdccqq
      421 llakspatrl gcqdgragar qvkrhpffks lnwkrleagm leppfvpdph avyakdvldi
      481 eqfstvkgvn ldasddsfys qfnmgsvsip wqtemietec fkelnvfgps qtrppdlcwe
      541 lppaedrrgc fpfrrkarsr ps
//
LOCUS       XP_077502075             520 aa            linear   INV 25-NOV-2025
DEFINITION  TWiK family of potassium channels protein 18-like [Amblyomma
            americanum].
ACCESSION   XP_077502075
VERSION     XP_077502075.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645949.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..520
                     /product="TWiK family of potassium channels protein
                     18-like"
                     /calculated_mol_wt=57777
     Region          <214..270
                     /region_name="Ion_trans_2"
                     /note="Ion channel; pfam07885"
                     /db_xref="CDD:462301"
     Region          367..437
                     /region_name="Ion_trans_2"
                     /note="Ion channel; pfam07885"
                     /db_xref="CDD:462301"
     CDS             1..520
                     /gene="LOC144113055"
                     /coded_by="XM_077645949.1:24..1586"
                     /db_xref="GeneID:144113055"
ORIGIN      
        1 mmrkrpargp pgprrwrggy fggrpawlrp sclgapglrp iwaaapsgaa eanmssrsps
       61 pragrrpafl sqpsviswqs dptrppqyli lnpmhivnlt adktrscavr aaqsvkafsq
      121 rwfshillll vllayaamga alfqliegpf edkqkqtiln arqyiiedlv raqrtlsvas
      181 frrhalrrlr efetqvrvle dearivshse kkvwsfwgal fycgtvftti gygniapstl
      241 ggmavtvlya figiplllmv ladlgklftr gikwlflslr qlfrtgrcgk hkakapppql
      301 qyvafvwrkv ndtmayvpyp aylkpepttp sgeeggeevs kaaseeelvi ddqfnlpvsl
      361 allllsiymt vgaciftiwe dwsffeafyf vfismstigf gdyvpdhpmf mmatfiyllf
      421 glaltsmcin vvqeklsaif erarmqlgtt igfdamvidq erkepletdi neavtkdaia
      481 keaiakglaa qeaaaksate kaavtndvst knssqhkept
//
LOCUS       XP_077502076             478 aa            linear   INV 25-NOV-2025
DEFINITION  AT-rich interactive domain-containing protein 5B-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077502076
VERSION     XP_077502076.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645950.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..478
                     /product="AT-rich interactive domain-containing protein
                     5B-like isoform X1"
                     /calculated_mol_wt=53833
     Region          30..>106
                     /region_name="BAH"
                     /note="BAH domain; pfam01426"
                     /db_xref="CDD:460207"
     Region          327..410
                     /region_name="ARID_ARID5"
                     /note="ARID/BRIGHT DNA binding domain of AT-rich
                     interactive domain-containing proteins ARID5A, ARID5B, and
                     similar proteins; cd16869"
                     /db_xref="CDD:350633"
     Site            order(345..351,376,378..379,382,393,395..397,399)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:350633"
     CDS             1..478
                     /gene="LOC144113056"
                     /coded_by="XM_077645950.1:125..1561"
                     /db_xref="GeneID:144113056"
ORIGIN      
        1 msfvgdprgr sgsctfygsf eyksrsgpat lsvgqfvyvr ispnddpcia elqllwkdhq
       61 hsaqlvsvql yflpeqtpdg rlphqgqhev laasekavlr ledfvgcitd evewshglla
      121 gppsggtssp gvvvlsypry cryrallkrl eghwppegsl alllgadqll pqganayrll
      181 fcrdtfdhea lahsdlicdh lapvergrqr krsrkansgs esseegssem itaaasaplk
      241 psvphskasq dkaahvklek vkfektslds ipknkpsqek kvqdkvpldr mphgrmsqdk
      301 lspdtvsqdr mphdkmlldk trngadeflr rlfqfmkern tpiqrvphlg fkqidlyhfy
      361 qfsqrlggye kitgrkqwkq vydqlggdps stsaatctrr hyerlllpfe lhlrgedrpp
      421 cwrleegpsd cqvldlsvrr eapppaqcld lrvhrrgden calqgsplys vyrledaw
//
LOCUS       XP_077502077             418 aa            linear   INV 25-NOV-2025
DEFINITION  AT-rich interactive domain-containing protein 5B-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077502077
VERSION     XP_077502077.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645951.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..418
                     /product="AT-rich interactive domain-containing protein
                     5B-like isoform X2"
                     /calculated_mol_wt=46686
     Region          30..>106
                     /region_name="BAH"
                     /note="BAH domain; pfam01426"
                     /db_xref="CDD:460207"
     Region          327..403
                     /region_name="ARID_ARID5"
                     /note="ARID/BRIGHT DNA binding domain of AT-rich
                     interactive domain-containing proteins ARID5A, ARID5B, and
                     similar proteins; cd16869"
                     /db_xref="CDD:350633"
     Site            order(345..351,376,378..379,382,393,395..397,399)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:350633"
     CDS             1..418
                     /gene="LOC144113056"
                     /coded_by="XM_077645951.1:127..1383"
                     /db_xref="GeneID:144113056"
ORIGIN      
        1 msfvgdprgr sgsctfygsf eyksrsgpat lsvgqfvyvr ispnddpcia elqllwkdhq
       61 hsaqlvsvql yflpeqtpdg rlphqgqhev laasekavlr ledfvgcitd evewshglla
      121 gppsggtssp gvvvlsypry cryrallkrl eghwppegsl alllgadqll pqganayrll
      181 fcrdtfdhea lahsdlicdh lapvergrqr krsrkansgs esseegssem itaaasaplk
      241 psvphskasq dkaahvklek vkfektslds ipknkpsqek kvqdkvpldr mphgrmsqdk
      301 lspdtvsqdr mphdkmlldk trngadeflr rlfqfmkern tpiqrvphlg fkqidlyhfy
      361 qfsqrlggye kitgrkqwkq vydqlggdps stsaatctrr hyesndldre acqasaai
//
LOCUS       XP_077502078             387 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein C2orf42 [Amblyomma americanum].
ACCESSION   XP_077502078
VERSION     XP_077502078.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645952.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..387
                     /product="uncharacterized protein C2orf42"
                     /calculated_mol_wt=43117
     Region          10..47
                     /region_name="zf-tcix"
                     /note="Putative treble-clef, zinc-finger, Zn-binding;
                     pfam14952"
                     /db_xref="CDD:464398"
     CDS             1..387
                     /gene="LOC144113057"
                     /coded_by="XM_077645952.1:136..1299"
                     /db_xref="GeneID:144113057"
ORIGIN      
        1 mssrkslfsd lgkatlrgir kcpkcgtyng trgvrcknka cdevfrehgv rkrgadavrl
       61 htsapgqlfs vrlqrgeart fvqlsaegsa lcegcqgssg cahvqaalls saqaqelplk
      121 psvveaeees vrdaiwnlva segsplvqrv srtvlavrrq ggfvhvrlsp rrqlrcadcg
      181 rskqgcvhsh acmcaltsad evradklrdl apkrpepslt flqwlsgvte rinetmrydc
      241 pgrpdplvfh vpqeffeclq qricgrrlpa rkdgvkctws itsllhvrhi fetpdvplee
      301 srafiencdg tyepyqppfv pdepacegvp lirplelktf lkvgnfphsa pfviewtpdv
      361 lprsrvgelr lmfeyghlrn ghveqrp
//
LOCUS       XP_077502079             384 aa            linear   INV 25-NOV-2025
DEFINITION  required for meiotic nuclear division 5 protein souji [Amblyomma
            americanum].
ACCESSION   XP_077502079
VERSION     XP_077502079.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645953.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..384
                     /product="required for meiotic nuclear division 5 protein
                     souji"
                     /calculated_mol_wt=42206
     Region          17..384
                     /region_name="COG5109"
                     /note="Uncharacterized conserved protein, contains RING
                     Zn-finger [General function prediction only]"
                     /db_xref="CDD:227440"
     CDS             1..384
                     /gene="Sou"
                     /coded_by="XM_077645953.1:1546..2700"
                     /db_xref="GeneID:144113058"
ORIGIN      
        1 mesclavere idkvlskfgt lsehtkttls eligyvqemy relgelpadt dvttshgial
       61 tqcaqkikdv ssslatehrd lhgtvskvgk aidknfvpdf watsseevfe gsdkktalnq
      121 vigehllrqg mldiaeelsr earlesaqke pfaelnnvld alkrrdlgpa lawvtqhelq
      181 gtalhfqlhr lhlvgllqrg aaaeaisyar ahlgplarqh erdlqvlmgs laflrvpggl
      241 arspyaflle pslwsdtcda ftrdacallg lsvrsplavc veagslalpa llnikqvmlq
      301 rqvagvwstr delpieirlg cqfhsvfacp ilrqqstdtn ppmrlvcghv isrdalhkla
      361 sgsklkcpyc pveqnptdar lihf
//
LOCUS       XP_077502080             376 aa            linear   INV 25-NOV-2025
DEFINITION  methyltransferase like 4 [Amblyomma americanum].
ACCESSION   XP_077502080
VERSION     XP_077502080.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645954.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..376
                     /product="methyltransferase like 4"
                     /calculated_mol_wt=41549
     Region          193..356
                     /region_name="MT-A70"
                     /note="cl01947"
                     /db_xref="CDD:470404"
     CDS             1..376
                     /gene="Mettl4"
                     /coded_by="XM_077645954.1:5..1135"
                     /db_xref="GeneID:144113059"
ORIGIN      
        1 maarmavlld cesgcfishn qlleswyrkc qrpgvtgdvs lklkdilfdf ktpirmdsaa
       61 drlaagterr krkrrsiddt pltdfvqpgs wiaeasgriv raaldaghle lgpasdcnvr
      121 dnnraardaa taallaaspp adgdssptpt gfrcpalhar liqfrgqpvw lpakaraqlg
      181 dvrdvgpyls gsydfilldp pwqnksakrk rtygtlrhde llsvlplgrl asprgclvav
      241 wctanpghlq fvarqllpsw gltylatwyw vkvtkhgepv rpfdcphkkp yefvlfggps
      301 sasvprdkvf vsvpscvhsh kpplselvrp ylrdgaecme lfarylvpgw tsvgnevlkl
      361 qhdsfyepts heslps
//
LOCUS       XP_077502081             285 aa            linear   INV 25-NOV-2025
DEFINITION  endoplasmic reticulum-Golgi intermediate compartment protein 1-like
            [Amblyomma americanum].
ACCESSION   XP_077502081
VERSION     XP_077502081.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645955.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..285
                     /product="endoplasmic reticulum-Golgi intermediate
                     compartment protein 1-like"
                     /calculated_mol_wt=31914
     Region          5..96
                     /region_name="ERGIC_N"
                     /note="Endoplasmic Reticulum-Golgi Intermediate
                     Compartment (ERGIC); pfam13850"
                     /db_xref="CDD:464000"
     Region          97..266
                     /region_name="COPIIcoated_ERV"
                     /note="Endoplasmic reticulum vesicle transporter;
                     pfam07970"
                     /db_xref="CDD:462327"
     CDS             1..285
                     /gene="LOC144113060"
                     /coded_by="XM_077645955.1:214..1071"
                     /db_xref="GeneID:144113060"
ORIGIN      
        1 mafdvrrfdi yrkipkdltq ptvtgavisi lscffisilf lsefisymsp elvselyvdn
       61 psstekipvs initllkldc svvgldiqdd mgrhevgfve ntektpvgpg crfegkffih
      121 kvpgnfhvst haaakqpeki dmthiihdlt fglkmtdevk gsfnsldemd ksgangiesh
      181 dyvmkivptv yeksrgerie syqytyayks yvsishtgri mpaiwfrydl tpitvkytrr
      241 gvplysflts vcaivggtft vagivdslvf tasevfkkfe mgkls
//
LOCUS       XP_077502082             312 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113061 [Amblyomma americanum].
ACCESSION   XP_077502082
VERSION     XP_077502082.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645956.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..312
                     /product="uncharacterized protein LOC144113061"
                     /calculated_mol_wt=34434
     Region          <27..212
                     /region_name="PRK12323"
                     /note="DNA polymerase III subunit gamma/tau; cl46901"
                     /db_xref="CDD:481241"
     Region          221..245
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          223..245
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(230,232,234,236..237,240..241,244,258,260,264..265,
                     268..269,272,286,288,290,292..293,296..297,300)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          248..>304
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          253..273
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          281..301
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..312
                     /gene="LOC144113061"
                     /coded_by="XM_077645956.1:140..1078"
                     /db_xref="GeneID:144113061"
ORIGIN      
        1 mtmdgldtls rdclqfnlsf esfkatgagg lappegsddp lntpvsttae litffatdav
       61 ipdpvpisge pahrwpaerg aasplrsdea kdpygrlayr gtfttawpgp dagpffeqpe
      121 pfppldsfka ppytaepepa kypwfayepp hapaagssaa aaakpeesta tlaeynqats
      181 kgheilsqay qnspvplklv pvkprkypnr psktpvherp yacpiegcdr rfsrsdeltr
      241 hirihtgqkp frcniclrsf srsdhltthi rthtgekpft cdacgrkfar sderkrhakv
      301 hlkhrlkked hp
//
LOCUS       XP_077502083             534 aa            linear   INV 25-NOV-2025
DEFINITION  F-box/WD repeat-containing protein lin-23-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077502083
VERSION     XP_077502083.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645957.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..534
                     /product="F-box/WD repeat-containing protein lin-23-like
                     isoform X1"
                     /calculated_mol_wt=60370
     Region          72..108
                     /region_name="Beta-TrCP_D"
                     /note="D domain of beta-TrCP; pfam12125"
                     /db_xref="CDD:463465"
     Region          108..158
                     /region_name="F-box_SF"
                     /note="F-box domain superfamily; cl45894"
                     /db_xref="CDD:459239"
     Site            order(120..124,127..128,131,142..144,146,149)
                     /site_type="other"
                     /note="F-box motif"
                     /db_xref="CDD:438954"
     Site            order(120,123,126..127,130..131,135,137..138,142..144,146)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438954"
     Region          233..511
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cd00200"
                     /db_xref="CDD:238121"
     Region          236..271
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Site            order(248,252,258..259,271..272,288,292,298..299,311..312,
                     327,332,338..339,355,370,375,381..382,394..395,411,415,
                     421..422,434..435,450,455,461..462,483..484,500,504,
                     510..511)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          277..311
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          316..353
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          360..393
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          399..433
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          439..482
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          488..510
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..534
                     /gene="LOC144113064"
                     /coded_by="XM_077645957.1:145..1749"
                     /db_xref="GeneID:144113064"
ORIGIN      
        1 mettlseekn vpgqgaerys astcpgrspc tcstpdaepv atqlcestkq csgtvaghaa
       61 sqadrerclv sfdtwpeevq tqfveallar mshcqhsris tflmpmlqrd fisllpkkgl
      121 dhvaeailsy ldarslcaae avcrewrrvv aegglwrklm errvstdplw rglaerrgwm
      181 qylfkpppge phpdhmfyrr lypriiqdie siennwrcgr hnlqrincrs enskgvyclq
      241 yddtkivsgl rdntikiwdr aslqcvkvlt ghtgsvlclq yddkviisgs sdstvrvwdv
      301 ktgemvntli hhceavlhlr fnngmmvtcs kdrsiavwdm vapreinlrr vlvghraavn
      361 vvdfderyvv sasgdrtikv wgtpncefvr tlnghkrgia clqyrdrlvv sgssdntirl
      421 wdiecgaclr vlegheelvr cirfdtkriv sgaydgkikv wdlaaaldpr apagtlclrt
      481 lvehsgrvfr lqfddfqivs sshddtiliw dflhlpseaa ssplpprtyt yvnk
//
LOCUS       XP_077502084             530 aa            linear   INV 25-NOV-2025
DEFINITION  F-box/WD repeat-containing protein lin-23-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077502084
VERSION     XP_077502084.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645958.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..530
                     /product="F-box/WD repeat-containing protein lin-23-like
                     isoform X2"
                     /calculated_mol_wt=60144
     Region          46..82
                     /region_name="Beta-TrCP_D"
                     /note="D domain of beta-TrCP; cl26390"
                     /db_xref="CDD:474793"
     Region          82..132
                     /region_name="F-box_SF"
                     /note="F-box domain superfamily; cl45894"
                     /db_xref="CDD:459239"
     Site            order(94..98,101..102,105,116..118,120,123)
                     /site_type="other"
                     /note="F-box motif"
                     /db_xref="CDD:438852"
     Site            order(94,97,100..101,104..105,109,111..112,116..118,120)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438852"
     Region          207..507
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cd00200"
                     /db_xref="CDD:238121"
     Region          210..245
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Site            order(222,226,232..233,245..246,262,266,272..273,285..286,
                     301,306,312..313,329,344,349,355..356,368..369,385,389,
                     395..396,408..409,424,429,435..436,479..480,496,500,
                     506..507)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          251..285
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          290..327
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          334..367
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          373..407
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          413..464
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          471..506
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..530
                     /gene="LOC144113064"
                     /coded_by="XM_077645958.1:139..1731"
                     /db_xref="GeneID:144113064"
ORIGIN      
        1 mettlseekn vpgqatqlce stkqcsgtva ghaasqadre rclvsfdtwp eevqtqfvea
       61 llarmshcqh sristflmpm lqrdfisllp kkgldhvaea ilsyldarsl caaeavcrew
      121 rrvvaegglw rklmerrvst dplwrglaer rgwmqylfkp ppgephpdhm fyrrlyprii
      181 qdiesiennw rcgrhnlqri ncrsenskgv yclqyddtki vsglrdntik iwdraslqcv
      241 kvltghtgsv lclqyddkvi isgssdstvr vwdvktgemv ntlihhceav lhlrfnngmm
      301 vtcskdrsia vwdmvaprei nlrrvlvghr aavnvvdfde ryvvsasgdr tikvwgtpnc
      361 efvrtlnghk rgiaclqyrd rlvvsgssdn tirlwdiecg aclrvleghe elvrcirfdt
      421 krivsgaydg kikvwdlaaa ldprapagtl clrtlvvrav cwcsqpclfl issmsasqeh
      481 sgrvfrlqfd dfqivssshd dtiliwdflh lpseaasspl pprtytyvnk
//
LOCUS       XP_077502085             518 aa            linear   INV 25-NOV-2025
DEFINITION  F-box/WD repeat-containing protein lin-23-like isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077502085
VERSION     XP_077502085.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645959.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..518
                     /product="F-box/WD repeat-containing protein lin-23-like
                     isoform X3"
                     /calculated_mol_wt=58788
     Region          34..70
                     /region_name="Beta-TrCP_D"
                     /note="D domain of beta-TrCP; pfam12125"
                     /db_xref="CDD:463465"
     Region          70..120
                     /region_name="F-box_SF"
                     /note="F-box domain superfamily; cl45894"
                     /db_xref="CDD:459239"
     Site            order(82..86,89..90,93,104..106,108,111)
                     /site_type="other"
                     /note="F-box motif"
                     /db_xref="CDD:438852"
     Site            order(82,85,88..89,92..93,97,99..100,104..106,108)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438852"
     Region          195..495
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cd00200"
                     /db_xref="CDD:238121"
     Region          198..233
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Site            order(210,214,220..221,233..234,250,254,260..261,273..274,
                     289,294,300..301,317,332,337,343..344,356..357,373,377,
                     383..384,396..397,412,417,423..424,467..468,484,488,
                     494..495)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          239..273
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          278..315
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          322..355
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          361..395
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          401..452
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          459..494
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..518
                     /gene="LOC144113064"
                     /coded_by="XM_077645959.1:76..1632"
                     /db_xref="GeneID:144113064"
ORIGIN      
        1 mgatqlcest kqcsgtvagh aasqadrerc lvsfdtwpee vqtqfveall armshcqhsr
       61 istflmpmlq rdfisllpkk gldhvaeail syldarslca aeavcrewrr vvaegglwrk
      121 lmerrvstdp lwrglaerrg wmqylfkppp gephpdhmfy rrlypriiqd iesiennwrc
      181 grhnlqrinc rsenskgvyc lqyddtkivs glrdntikiw draslqcvkv ltghtgsvlc
      241 lqyddkviis gssdstvrvw dvktgemvnt lihhceavlh lrfnngmmvt cskdrsiavw
      301 dmvapreinl rrvlvghraa vnvvdfdery vvsasgdrti kvwgtpncef vrtlnghkrg
      361 iaclqyrdrl vvsgssdnti rlwdiecgac lrvlegheel vrcirfdtkr ivsgaydgki
      421 kvwdlaaald prapagtlcl rtlvvravcw csqpclflis smsasqehsg rvfrlqfddf
      481 qivssshddt iliwdflhlp seaassplpp rtytyvnk
//
LOCUS       XP_077502086             508 aa            linear   INV 25-NOV-2025
DEFINITION  F-box/WD repeat-containing protein lin-23-like isoform X4
            [Amblyomma americanum].
ACCESSION   XP_077502086
VERSION     XP_077502086.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645960.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..508
                     /product="F-box/WD repeat-containing protein lin-23-like
                     isoform X4"
                     /calculated_mol_wt=57745
     Region          46..82
                     /region_name="Beta-TrCP_D"
                     /note="D domain of beta-TrCP; pfam12125"
                     /db_xref="CDD:463465"
     Region          82..132
                     /region_name="F-box_SF"
                     /note="F-box domain superfamily; cl45894"
                     /db_xref="CDD:459239"
     Site            order(94..98,101..102,105,116..118,120,123)
                     /site_type="other"
                     /note="F-box motif"
                     /db_xref="CDD:438852"
     Site            order(94,97,100..101,104..105,109,111..112,116..118,120)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438852"
     Region          207..485
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cd00200"
                     /db_xref="CDD:238121"
     Region          210..245
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Site            order(222,226,232..233,245..246,262,266,272..273,285..286,
                     301,306,312..313,329,344,349,355..356,368..369,385,389,
                     395..396,408..409,424,429,435..436,457..458,474,478,
                     484..485)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          251..285
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          290..327
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          334..367
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          373..407
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          413..456
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          462..484
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..508
                     /gene="LOC144113064"
                     /coded_by="XM_077645960.1:139..1665"
                     /db_xref="GeneID:144113064"
ORIGIN      
        1 mettlseekn vpgqatqlce stkqcsgtva ghaasqadre rclvsfdtwp eevqtqfvea
       61 llarmshcqh sristflmpm lqrdfisllp kkgldhvaea ilsyldarsl caaeavcrew
      121 rrvvaegglw rklmerrvst dplwrglaer rgwmqylfkp ppgephpdhm fyrrlyprii
      181 qdiesiennw rcgrhnlqri ncrsenskgv yclqyddtki vsglrdntik iwdraslqcv
      241 kvltghtgsv lclqyddkvi isgssdstvr vwdvktgemv ntlihhceav lhlrfnngmm
      301 vtcskdrsia vwdmvaprei nlrrvlvghr aavnvvdfde ryvvsasgdr tikvwgtpnc
      361 efvrtlnghk rgiaclqyrd rlvvsgssdn tirlwdiecg aclrvleghe elvrcirfdt
      421 krivsgaydg kikvwdlaaa ldprapagtl clrtlvehsg rvfrlqfddf qivssshddt
      481 iliwdflhlp seaassplpp rtytyvnk
//
LOCUS       XP_077502087             556 aa            linear   INV 25-NOV-2025
DEFINITION  F-box/WD repeat-containing protein lin-23-like isoform X5
            [Amblyomma americanum].
ACCESSION   XP_077502087
VERSION     XP_077502087.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645961.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..556
                     /product="F-box/WD repeat-containing protein lin-23-like
                     isoform X5"
                     /calculated_mol_wt=62769
     Region          72..108
                     /region_name="Beta-TrCP_D"
                     /note="D domain of beta-TrCP; pfam12125"
                     /db_xref="CDD:463465"
     Region          108..158
                     /region_name="F-box_SF"
                     /note="F-box domain superfamily; cl45894"
                     /db_xref="CDD:459239"
     Site            order(120..124,127..128,131,142..144,146,149)
                     /site_type="other"
                     /note="F-box motif"
                     /db_xref="CDD:438852"
     Site            order(120,123,126..127,130..131,135,137..138,142..144,146)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438852"
     Region          233..533
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cd00200"
                     /db_xref="CDD:238121"
     Region          236..271
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Site            order(248,252,258..259,271..272,288,292,298..299,311..312,
                     327,332,338..339,355,370,375,381..382,394..395,411,415,
                     421..422,434..435,450,455,461..462,505..506,522,526,
                     532..533)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          277..311
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          316..353
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          360..393
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          399..433
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          439..490
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          497..532
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..556
                     /gene="LOC144113064"
                     /coded_by="XM_077645961.1:157..1827"
                     /db_xref="GeneID:144113064"
ORIGIN      
        1 mettlseekn vpgqgaerys astcpgrspc tcstpdaepv atqlcestkq csgtvaghaa
       61 sqadrerclv sfdtwpeevq tqfveallar mshcqhsris tflmpmlqrd fisllpkkgl
      121 dhvaeailsy ldarslcaae avcrewrrvv aegglwrklm errvstdplw rglaerrgwm
      181 qylfkpppge phpdhmfyrr lypriiqdie siennwrcgr hnlqrincrs enskgvyclq
      241 yddtkivsgl rdntikiwdr aslqcvkvlt ghtgsvlclq yddkviisgs sdstvrvwdv
      301 ktgemvntli hhceavlhlr fnngmmvtcs kdrsiavwdm vapreinlrr vlvghraavn
      361 vvdfderyvv sasgdrtikv wgtpncefvr tlnghkrgia clqyrdrlvv sgssdntirl
      421 wdiecgaclr vlegheelvr cirfdtkriv sgaydgkikv wdlaaaldpr apagtlclrt
      481 lvvravcwcs qpclflissm sasqehsgrv frlqfddfqi vssshddtil iwdflhlpse
      541 aassplpprt ytyvnk
//
LOCUS       XP_077502088            1136 aa            linear   INV 25-NOV-2025
DEFINITION  ran-binding protein 16 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502088
VERSION     XP_077502088.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645962.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1136
                     /product="ran-binding protein 16 isoform X1"
                     /calculated_mol_wt=127554
     Region          29..95
                     /region_name="IBN_N"
                     /note="Importin-beta N-terminal domain; smart00913"
                     /db_xref="CDD:197981"
     CDS             1..1136
                     /gene="Ranbp16"
                     /coded_by="XM_077645962.1:114..3524"
                     /db_xref="GeneID:144113065"
ORIGIN      
        1 maddqelarv ealcqqlyeg gdaaeraqae sqlsefccgs spdclqrcrl lldrsqssya
       61 qllaattlsk lvsknpgsls lqqrlemrny ilsylwqrpk gaayvtqalv qlfarltklg
      121 wfdtdkeefv frnvirqvqs flqgsveycm vgvqllsqlt cemnhvteae anrsltkqrk
      181 iassfrdsql ydifqlacdl lrraleswks qmtynddtqq qlmsqllrla hhclsfdfig
      241 tspdessddl ctvqiptgwr pafldyntlq lffdlfhslp gtlsplvltc lvqiasvrrs
      301 lfnnaerakf lsqlvtgvkh ilenpqslsd pnsyhefcrl larlksnyql gelvkvetyp
      361 etialiakft vtslqmwqfa pnsvhyllsl wqrmvasvpy vkasephlld aytpevtray
      421 itsrldsvsq vmsfmhpags ghrhvvctts gsveatlvpa nregledpld dmgmvqqqld
      481 qlstigrcey ektcallvql ldqsaqayre agtqgaaald lailegqlsw lvyiigaaig
      541 grasfstadd hdamdgelvc rvlqlmqlrd srlplgscsg eklelallsf feqfrkiyvg
      601 dqvpktskvy rrlsevlgls ddsmvlsvfv rkiitnlkyw sysdaivskt lqllsdlsvg
      661 yssvrklvkl eevqfmlsnh tsehfpflgh nsgnrspcap aagsagsagg grergmdmrc
      721 rstfytslgr llmvelgede erfvqfmrpl taafeaigat lanadsplfn adeakkmlig
      781 lardlrglvf afntktsymm lfewiyptyf pilhralelw yhdpsvstpv lklvaelvqn
      841 rsqrlhfdvs spngillfre tsrtmvlygt ciltlgqipk dqvyklklkg vaicfsmlks
      901 alcggyvnlg vfslygdsal ddalgifvkl llsipqpdll dypklsqayy vlleclaqdh
      961 mhflsnlepq vflyilssvs egltaldtmv ctgccatldh mvsflfrrls kgstkgppep
     1021 clrvlelhpe ilqqmlstil niimfedcrn qwsmsrpllg lillneeyfg qlrqslvssq
     1081 pvekqatmsq wfdslmdgie rnlltknrdr ftqnlsvfrr dindslkgps tldmmt
//
LOCUS       XP_077502089            1107 aa            linear   INV 25-NOV-2025
DEFINITION  ran-binding protein 16 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502089
VERSION     XP_077502089.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645963.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1107
                     /product="ran-binding protein 16 isoform X2"
                     /calculated_mol_wt=124622
     Region          29..95
                     /region_name="IBN_N"
                     /note="Importin-beta N-terminal domain; smart00913"
                     /db_xref="CDD:197981"
     CDS             1..1107
                     /gene="Ranbp16"
                     /coded_by="XM_077645963.1:114..3437"
                     /db_xref="GeneID:144113065"
ORIGIN      
        1 maddqelarv ealcqqlyeg gdaaeraqae sqlsefccgs spdclqrcrl lldrsqssya
       61 qllaattlsk lvsknpgsls lqqrlemrny ilsylwqrpk gaayvtqalv qlfarltklg
      121 wfdtdkeefv frnvirqvqs flqgsveycm vgvqllsqlt cemnhvteae anrsltkqrk
      181 iassfrdsql ydifqlacdl lrraleswks qmtynddtqq qlmsqllrla hhclsfdfig
      241 tspdessddl ctvqiptgwr pafldyntlq lffdlfhslp gtlsplvltc lvqiasvrrs
      301 lfnnaerakf lsqlvtgvkh ilenpqslsd pnsyhefcrl larlksnyql gelvkvetyp
      361 etialiakft vtslqmwqfa pnsvhyllsl wqrmvasvpy vkasephlld aytpevtray
      421 itsrldsvsq vmregledpl ddmgmvqqql dqlstigrce yektcallvq lldqsaqayr
      481 eagtqgaaal dlailegqls wlvyiigaai ggrasfstad dhdamdgelv crvlqlmqlr
      541 dsrlplgscs geklelalls ffeqfrkiyv gdqvpktskv yrrlsevlgl sddsmvlsvf
      601 vrkiitnlky wsysdaivsk tlqllsdlsv gyssvrklvk leevqfmlsn htsehfpflg
      661 hnsgnrspca paagsagsag ggrergmdmr crstfytslg rllmvelged eerfvqfmrp
      721 ltaafeaiga tlanadsplf nadeakkmli glardlrglv fafntktsym mlfewiypty
      781 fpilhralel wyhdpsvstp vlklvaelvq nrsqrlhfdv sspngillfr etsrtmvlyg
      841 tciltlgqip kdqvyklklk gvaicfsmlk salcggyvnl gvfslygdsa lddalgifvk
      901 lllsipqpdl ldypklsqay yvlleclaqd hmhflsnlep qvflyilssv segltaldtm
      961 vctgccatld hmvsflfrrl skgstkgppe pclrvlelhp eilqqmlsti lniimfedcr
     1021 nqwsmsrpll glillneeyf gqlrqslvss qpvekqatms qwfdslmdgi ernlltknrd
     1081 rftqnlsvfr rdindslkgp stldmmt
//
LOCUS       XP_077502090            1106 aa            linear   INV 25-NOV-2025
DEFINITION  ran-binding protein 16 isoform X3 [Amblyomma americanum].
ACCESSION   XP_077502090
VERSION     XP_077502090.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645964.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1106
                     /product="ran-binding protein 16 isoform X3"
                     /calculated_mol_wt=124494
     Region          29..95
                     /region_name="IBN_N"
                     /note="Importin-beta N-terminal domain; smart00913"
                     /db_xref="CDD:197981"
     CDS             1..1106
                     /gene="Ranbp16"
                     /coded_by="XM_077645964.1:114..3434"
                     /db_xref="GeneID:144113065"
ORIGIN      
        1 maddqelarv ealcqqlyeg gdaaeraqae sqlsefccgs spdclqrcrl lldrsqssya
       61 qllaattlsk lvsknpgsls lqqrlemrny ilsylwqrpk gaayvtqalv qlfarltklg
      121 wfdtdkeefv frnvirqvqs flqgsveycm vgvqllsqlt cemnhvteae anrsltkqrk
      181 iassfrdsql ydifqlacdl lrraleswks qmtynddtqq lmsqllrlah hclsfdfigt
      241 spdessddlc tvqiptgwrp afldyntlql ffdlfhslpg tlsplvltcl vqiasvrrsl
      301 fnnaerakfl sqlvtgvkhi lenpqslsdp nsyhefcrll arlksnyqlg elvkvetype
      361 tialiakftv tslqmwqfap nsvhyllslw qrmvasvpyv kasephllda ytpevtrayi
      421 tsrldsvsqv mregledpld dmgmvqqqld qlstigrcey ektcallvql ldqsaqayre
      481 agtqgaaald lailegqlsw lvyiigaaig grasfstadd hdamdgelvc rvlqlmqlrd
      541 srlplgscsg eklelallsf feqfrkiyvg dqvpktskvy rrlsevlgls ddsmvlsvfv
      601 rkiitnlkyw sysdaivskt lqllsdlsvg yssvrklvkl eevqfmlsnh tsehfpflgh
      661 nsgnrspcap aagsagsagg grergmdmrc rstfytslgr llmvelgede erfvqfmrpl
      721 taafeaigat lanadsplfn adeakkmlig lardlrglvf afntktsymm lfewiyptyf
      781 pilhralelw yhdpsvstpv lklvaelvqn rsqrlhfdvs spngillfre tsrtmvlygt
      841 ciltlgqipk dqvyklklkg vaicfsmlks alcggyvnlg vfslygdsal ddalgifvkl
      901 llsipqpdll dypklsqayy vlleclaqdh mhflsnlepq vflyilssvs egltaldtmv
      961 ctgccatldh mvsflfrrls kgstkgppep clrvlelhpe ilqqmlstil niimfedcrn
     1021 qwsmsrpllg lillneeyfg qlrqslvssq pvekqatmsq wfdslmdgie rnlltknrdr
     1081 ftqnlsvfrr dindslkgps tldmmt
//
LOCUS       XP_077502091             137 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113067 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502091
VERSION     XP_077502091.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645965.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..137
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..137
                     /product="uncharacterized protein LOC144113067 isoform X1"
                     /calculated_mol_wt=14648
     CDS             1..137
                     /gene="LOC144113067"
                     /coded_by="XM_077645965.1:953..1366"
                     /db_xref="GeneID:144113067"
ORIGIN      
        1 mvlcnrdtei ehsfpmvlss egaentdssc ttavqapqfl tkhseqerdi agillelgsl
       61 krctenkavq vksggfpkrf admcgavgep ahqlrarrpk gngaarkrss atrspglgaq
      121 pcfegqesaa mktkter
//
LOCUS       XP_077502092              85 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113067 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502092
VERSION     XP_077502092.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645966.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..85
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..85
                     /product="uncharacterized protein LOC144113067 isoform X2"
                     /calculated_mol_wt=9196
     CDS             1..85
                     /gene="LOC144113067"
                     /coded_by="XM_077645966.1:953..1210"
                     /db_xref="GeneID:144113067"
ORIGIN      
        1 mvlcnrdtei ehsfpmvlss egaentdssc ttavqapqfl tkhseqerdi agillelgsl
       61 krctenkavq vksggfpkrf admvp
//
LOCUS       XP_077502094             794 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113069 [Amblyomma americanum].
ACCESSION   XP_077502094
VERSION     XP_077502094.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645968.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..794
                     /product="uncharacterized protein LOC144113069"
                     /calculated_mol_wt=88528
     Region          105..135
                     /region_name="EGF"
                     /note="Epidermal growth factor-like domain; smart00181"
                     /db_xref="CDD:214544"
     CDS             1..794
                     /gene="LOC144113069"
                     /coded_by="XM_077645968.1:164..2548"
                     /db_xref="GeneID:144113069"
ORIGIN      
        1 mgvvwsskps scmhattlll aaffcctvls fepdpahyst pheaieyaaw malkegkppc
       61 vtvhpigitl fcimgqcvqd kngssycqcr qhfagltcsv ykgkctsetl csphlckdnp
      121 skislyecvc kpgyhvpdsp afshcipdnl lgsatrerds tktakrhsae knshlnfsvs
      181 ntttstisvf hkeekapqps vvcktsdfrt sfsepkegrd sffgqtsprt dskmiralta
      241 rteetktsss salhdienqq gslpnkdksk nnakvrmqar svmkpymsls nasitsqhig
      301 remppltpqh yskqavnvqm ptirrrrast fqreqgqape nqlilddlts ysarsknena
      361 gkkknsklis ifnsehnmpv fghasdkgrn hehitsstev alevpqreyq kegdedyevs
      421 qfkkkllnma paqlassegh idnslqgpaa ylvaagmkrv lggkkdvkhk qlpaeaqdei
      481 idslkyavea sensktnete sdsemkkgni lvvadkrkrr kssviqsdyv aedngtnqrl
      541 pfliaeplla tknrkdipvp telhrviqal mqhsseeshl qgatyrhden ygtkmkhlkn
      601 sampamtwrk dlpprkgdem tpstsdtssk kpkklkkfks sdnansksew mnqnpwsevq
      661 nqalhsskdc vstncdnsli lmshvnyqaf nttnhpfskq stweaikntt nfsymkhmsp
      721 ardiskseap essseayakr kktkhrrsfg rdpklffnvt vlrsggrnks asylvpfags
      781 kailiswgty ndsl
//
LOCUS       XP_077502095             574 aa            linear   INV 25-NOV-2025
DEFINITION  lipase maturation factor 1-like [Amblyomma americanum].
ACCESSION   XP_077502095
VERSION     XP_077502095.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645969.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..574
                     /product="lipase maturation factor 1-like"
                     /calculated_mol_wt=65890
     Region          182..551
                     /region_name="LMF1"
                     /note="Lipase maturation factor; pfam06762"
                     /db_xref="CDD:462004"
     CDS             1..574
                     /gene="LOC144113070"
                     /coded_by="XM_077645969.1:120..1844"
                     /db_xref="GeneID:144113070"
ORIGIN      
        1 marlrknseq pplgkaasvs dkrdvpkdsg dlhsaerree gskkshlsal dtatthvllk
       61 nqywltrivf lrslgfvyaa aflvalnqnk yllgergllp annyleritd vhgglhietv
      121 lrvptlmwly kyvsvdtlld ciasvgtvla vgiavtgaan avslfllwil yhslvnigql
      181 wysfgwesql letgflaiff cpiwnwqqlp cetppspvvi wgyrwllfri mlgaglikir
      241 gdrcwweltc mmyhyetqpv pnplsyylhq tppfvhmlev ignhiielii pwfmfltrpf
      301 riacgiiqis fqiililsgn lsflnwltil psipyfddaa lrwifskkts nqvallakdt
      361 eegklqpskp rkatnlalal clgflsipvv vnlfsphqqm ntsfeplrlv ntygafgsit
      421 krraeviiqg thsedphnks avweeyeffc kpgnvfrrpc vispyhyrld wlmwfaafqd
      481 yqshpwlfhf aakllvndrn iskiisknpf mdrepprfir avryryryap igssqakagh
      541 wwirsrmgsy mypvslsslk pllknfgwkv psvk
//
LOCUS       XP_077502096             139 aa            linear   INV 25-NOV-2025
DEFINITION  protein FAM136A-like [Amblyomma americanum].
ACCESSION   XP_077502096
VERSION     XP_077502096.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645970.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..139
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..139
                     /product="protein FAM136A-like"
                     /calculated_mol_wt=15707
     Region          5..130
                     /region_name="DUF842"
                     /note="Eukaryotic protein of unknown function (DUF842);
                     pfam05811"
                     /db_xref="CDD:461747"
     CDS             1..139
                     /gene="LOC144113071"
                     /coded_by="XM_077645970.1:148..567"
                     /db_xref="GeneID:144113071"
ORIGIN      
        1 maeeaarrvq tavdnmvnel egthlrrilg nvhrcavkcc ddsrlsmega rtcimncsep
       61 iekahskvev elgnfqerir mcvvqcendv rdqfnpqtte aeamkfrgqy dacvmkcadk
      121 higllpqllq rlkdslkhc
//
LOCUS       XP_077502097             268 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113072 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502097
VERSION     XP_077502097.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645971.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..268
                     /product="uncharacterized protein LOC144113072 isoform X1"
                     /calculated_mol_wt=30740
     CDS             1..268
                     /gene="LOC144113072"
                     /coded_by="XM_077645971.1:8..814"
                     /db_xref="GeneID:144113072"
ORIGIN      
        1 matmsvmtht lrtcimnqaa wthfsasfir faglmfpldt rtwvtvlavl fhsamsapvs
       61 rsgenndhpl wvnpcnlpdd asghaghlrp qdvrnmldav anrarvasee aeeakrkfve
      121 rtfsdsefed svasyrlpwl pnvttpseeh fksmtkeeay kttfgyvqyy avgleqavld
      181 qifhngpshp lflkiqenlg qllcelqigi vhfnvaknpd vlrevmshey rdlkqdsqrn
      241 lrdyiilrey irltryisel faylrdhs
//
LOCUS       XP_077502098             250 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113072 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502098
VERSION     XP_077502098.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645972.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..250
                     /product="uncharacterized protein LOC144113072 isoform X2"
                     /calculated_mol_wt=28637
     CDS             1..250
                     /gene="LOC144113072"
                     /coded_by="XM_077645972.1:80..832"
                     /db_xref="GeneID:144113072"
ORIGIN      
        1 msvlndkpsr cgrrlkgkca dtrtwvtvla vlfhsamsap vsrsgenndh plwvnpcnlp
       61 ddasghaghl rpqdvrnmld avanrarvas eeaeeakrkf vertfsdsef edsvasyrlp
      121 wlpnvttpse ehfksmtkee aykttfgyvq yyavgleqav ldqifhngps hplflkiqen
      181 lgqllcelqi givhfnvakn pdvlrevmsh eyrdlkqdsq rnlrdyiilr eyirltryis
      241 elfaylrdhs
//
LOCUS       XP_077502099             235 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113072 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502099
VERSION     XP_077502099.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645973.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..235
                     /product="uncharacterized protein LOC144113072 isoform X3"
                     /calculated_mol_wt=27095
     CDS             1..235
                     /gene="LOC144113072"
                     /coded_by="XM_077645973.1:82..789"
                     /db_xref="GeneID:144113072"
ORIGIN      
        1 mrphhdtrtw vtvlavlfhs amsapvsrsg enndhplwvn pcnlpddasg haghlrpqdv
       61 rnmldavanr arvaseeaee akrkfvertf sdsefedsva syrlpwlpnv ttpseehfks
      121 mtkeeayktt fgyvqyyavg leqavldqif hngpshplfl kiqenlgqll celqigivhf
      181 nvaknpdvlr evmsheyrdl kqdsqrnlrd yiilreyirl tryiselfay lrdhs
//
LOCUS       XP_077502100             265 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113072 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077502100
VERSION     XP_077502100.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645974.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..265
                     /product="uncharacterized protein LOC144113072 isoform X4"
                     /calculated_mol_wt=30308
     CDS             1..265
                     /gene="LOC144113072"
                     /coded_by="XM_077645974.1:210..1007"
                     /db_xref="GeneID:144113072"
ORIGIN      
        1 mksrvarvpg kepccaarpr prwahhrpas sllpadtrtw vtvlavlfhs amsapvsrsg
       61 enndhplwvn pcnlpddasg haghlrpqdv rnmldavanr arvaseeaee akrkfvertf
      121 sdsefedsva syrlpwlpnv ttpseehfks mtkeeayktt fgyvqyyavg leqavldqif
      181 hngpshplfl kiqenlgqll celqigivhf nvaknpdvlr evmsheyrdl kqdsqrnlrd
      241 yiilreyirl tryiselfay lrdhs
//
LOCUS       XP_077502101             214 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113072 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077502101
VERSION     XP_077502101.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645975.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..214
                     /product="uncharacterized protein LOC144113072 isoform X5"
                     /calculated_mol_wt=24638
     CDS             1..214
                     /gene="LOC144113072"
                     /coded_by="XM_077645975.1:409..1053"
                     /db_xref="GeneID:144113072"
ORIGIN      
        1 msapvsrsge nndhplwvnp cnlpddasgh aghlrpqdvr nmldavanra rvaseeaeea
       61 krkfvertfs dsefedsvas yrlpwlpnvt tpseehfksm tkeeaykttf gyvqyyavgl
      121 eqavldqifh ngpshplflk iqenlgqllc elqigivhfn vaknpdvlre vmsheyrdlk
      181 qdsqrnlrdy iilreyirlt ryiselfayl rdhs
//
LOCUS       XP_077502102             214 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113072 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077502102
VERSION     XP_077502102.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645976.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..214
                     /product="uncharacterized protein LOC144113072 isoform X5"
                     /calculated_mol_wt=24638
     CDS             1..214
                     /gene="LOC144113072"
                     /coded_by="XM_077645976.1:150..794"
                     /db_xref="GeneID:144113072"
ORIGIN      
        1 msapvsrsge nndhplwvnp cnlpddasgh aghlrpqdvr nmldavanra rvaseeaeea
       61 krkfvertfs dsefedsvas yrlpwlpnvt tpseehfksm tkeeaykttf gyvqyyavgl
      121 eqavldqifh ngpshplflk iqenlgqllc elqigivhfn vaknpdvlre vmsheyrdlk
      181 qdsqrnlrdy iilreyirlt ryiselfayl rdhs
//
LOCUS       XP_077502103             968 aa            linear   INV 25-NOV-2025
DEFINITION  regulator of telomere elongation helicase 1 homolog [Amblyomma
            americanum].
ACCESSION   XP_077502103
VERSION     XP_077502103.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645977.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..968
                     /product="regulator of telomere elongation helicase 1
                     homolog"
                     /calculated_mol_wt=107893
     Region          12..720
                     /region_name="rad3"
                     /note="DNA repair helicase (rad3); TIGR00604"
                     /db_xref="CDD:273169"
     CDS             1..968
                     /gene="LOC144113073"
                     /coded_by="XM_077645977.1:452..3358"
                     /db_xref="GeneID:144113073"
ORIGIN      
        1 mslqmfnlrg vtvhfphepy dvqkkymekv ieclqsgvng ilesptgtgk tlsllcssla
       61 wledhkaamq ltaglhrspd pnagflsqlq slfdqqeaan rpspicpkii yssrthsqls
      121 qainelkktn yryvksvvlg srdqlcinpd vqklqdnask lrvcrhkvtt rtcpfhlnyd
      181 tkmtrseyqd tpvmdiedlg klgkkfvccp yyaaktlkgr adivfmpyny lvdaksrkah
      241 gvelegnvvi fdeahnienm ceesmsfqll ssdlalcike tthaadlkqq keteaaagme
      301 gvdpdftlld iakikaills lekyvdellv qvnaesttkp gnfmftmlee agvsrhnkde
      361 lldlldkivs flevnavgaf sprgtglnrf vnilnslysv egdgssveai fkkkfkvhiq
      421 kdankkkkps hdvwtvssna skkldwclnc wcfspsvsmd nllkqgvrci iltsgtlspl
      481 ssfaaelgip fpvqlenphv ikeeqiyvsv lsngydgqll ncsydnrnnp aylaslgrtv
      541 cnlcrvipgg vllffpsyav mrnfvetwta ngtmtslalv kptvmevqrn tdfssliqeh
      601 cenvdspekr gcllmavcrg rmsegmdftd qyaraaiivg fplppcfdpr vqlkkqylde
      661 spsrsifsgn dwyvlqatra vnqaigrvir hqhdfgailf cdkryseprn lsqlskwvke
      721 ktklrssfsv vlkelaaffk aagvsnensq agtkmhvasr nafgipskdg tsvsrnltsa
      781 qhsvaennen vmeayrrpte eqlasfhkve qgqnlfdvln nssapgavdf ssthkvnfrn
      841 tddkqtnedr lqnakrrkly vplkgigppe psmqdgasts rtsspkssqd iwksilaslk
      901 kslkecdyns vcssmklflr tnnsdalaea lalclvdapn rdelltclak vvtaskredf
      961 vvkcrshw
//
LOCUS       XP_077502104             342 aa            linear   INV 25-NOV-2025
DEFINITION  Kv channel-interacting protein 4-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502104
VERSION     XP_077502104.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645978.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..342
                     /product="Kv channel-interacting protein 4-like isoform
                     X1"
                     /calculated_mol_wt=38379
     Region          <211..327
                     /region_name="FRQ1"
                     /note="Ca2+-binding protein, EF-hand superfamily [Signal
                     transduction mechanisms]; COG5126"
                     /db_xref="CDD:444056"
     CDS             1..342
                     /gene="LOC144113074"
                     /coded_by="XM_077645978.1:167..1195"
                     /db_xref="GeneID:144113074"
ORIGIN      
        1 mstrsqdpaa vplagssglq qrrfagrsad raaspeaaaa pavtdgkadr arrvpsvgqs
       61 crparerrss afrrmrstfl asppcrrksa figagnkgsd nddvdeetev knfkywanki
      121 ghvfkkvrqt kkrrkssygs lfssldseid elegptvhyk peaietlcql tkfnkrelql
      181 myrgfkqecp sgmvkeetfk iiysqffprg adasqyahfv fntfdqdhtg aitftdfvig
      241 lsvlsrgslq eklrwtfnly dingdgyitk delarivkav ydlmgkavep mveenttreh
      301 vervfqkldl nkdgvvtide fmdsctkden isksmsvldt il
//
LOCUS       XP_077502105             295 aa            linear   INV 25-NOV-2025
DEFINITION  Kv channel-interacting protein 4-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502105
VERSION     XP_077502105.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645979.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..295
                     /product="Kv channel-interacting protein 4-like isoform
                     X2"
                     /calculated_mol_wt=33147
     Region          <164..280
                     /region_name="FRQ1"
                     /note="Ca2+-binding protein, EF-hand superfamily [Signal
                     transduction mechanisms]; COG5126"
                     /db_xref="CDD:444056"
     CDS             1..295
                     /gene="LOC144113074"
                     /coded_by="XM_077645979.1:16..903"
                     /db_xref="GeneID:144113074"
ORIGIN      
        1 mctsaahmla ghrrhaglct wqspgdsfaa sppppwshrn rarkegrrrp lehrdarqci
       61 tgsrrsmqsl gtsggsssaa vrrtnarrtv sasgsqthns eidelegptv hykpeaietl
      121 cqltkfnkre lqlmyrgfkq ecpsgmvkee tfkiiysqff prgadasqya hfvfntfdqd
      181 htgaitftdf viglsvlsrg slqeklrwtf nlydingdgy itkdelariv kavydlmgka
      241 vepmveentt rehvervfqk ldlnkdgvvt idefmdsctk denisksmsv ldtil
//
LOCUS       XP_077502106             286 aa            linear   INV 25-NOV-2025
DEFINITION  Kv channel-interacting protein 4-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502106
VERSION     XP_077502106.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645980.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..286
                     /product="Kv channel-interacting protein 4-like isoform
                     X3"
                     /calculated_mol_wt=32265
     Region          <155..271
                     /region_name="FRQ1"
                     /note="Ca2+-binding protein, EF-hand superfamily [Signal
                     transduction mechanisms]; COG5126"
                     /db_xref="CDD:444056"
     CDS             1..286
                     /gene="LOC144113074"
                     /coded_by="XM_077645980.1:166..1026"
                     /db_xref="GeneID:144113074"
ORIGIN      
        1 mstrsqdpaa vplagssglq qrrfagrsad raaspegagn kgsdnddvde etevknfkyw
       61 ankighvfkk vrqtkkrrks sygslfssld seidelegpt vhykpeaiet lcqltkfnkr
      121 elqlmyrgfk qecpsgmvke etfkiiysqf fprgadasqy ahfvfntfdq dhtgaitftd
      181 fviglsvlsr gslqeklrwt fnlydingdg yitkdelari vkavydlmgk avepmveent
      241 trehvervfq kldlnkdgvv tidefmdsct kdenisksms vldtil
//
LOCUS       XP_077502107             279 aa            linear   INV 25-NOV-2025
DEFINITION  Kv channel-interacting protein 4-like isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077502107
VERSION     XP_077502107.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645981.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..279
                     /product="Kv channel-interacting protein 4-like isoform
                     X4"
                     /calculated_mol_wt=31348
     Region          <148..264
                     /region_name="FRQ1"
                     /note="Ca2+-binding protein, EF-hand superfamily [Signal
                     transduction mechanisms]; COG5126"
                     /db_xref="CDD:444056"
     CDS             1..279
                     /gene="LOC144113074"
                     /coded_by="XM_077645981.1:70..909"
                     /db_xref="GeneID:144113074"
ORIGIN      
        1 mygmvssqsg gkgrslrrhq lsrqrtshsl gvpagsprai pgcsseqpps parssshrgq
       61 hnvygrlvgf lkhtwtgftm avdseidele gptvhykpea ietlcqltkf nkrelqlmyr
      121 gfkqecpsgm vkeetfkiiy sqffprgada sqyahfvfnt fdqdhtgait ftdfviglsv
      181 lsrgslqekl rwtfnlydin gdgyitkdel arivkavydl mgkavepmve enttrehver
      241 vfqkldlnkd gvvtidefmd sctkdenisk smsvldtil
//
LOCUS       XP_077502108             440 aa            linear   INV 25-NOV-2025
DEFINITION  ADP-ribosylhydrolase ARH3-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502108
VERSION     XP_077502108.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645982.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..440
                     /product="ADP-ribosylhydrolase ARH3-like isoform X1"
                     /calculated_mol_wt=48834
     Region          86..437
                     /region_name="DraG"
                     /note="ADP-ribosylglycohydrolase [Posttranslational
                     modification, protein turnover, chaperones]; COG1397"
                     /db_xref="CDD:441007"
     CDS             1..440
                     /gene="LOC144113076"
                     /coded_by="XM_077645982.1:16..1338"
                     /db_xref="GeneID:144113076"
ORIGIN      
        1 mfigikaavt frfcqrkail peaevveeee ekkcgrrscr sekskkreiq hrddhfglpe
       61 krerdvfgkw dgipgtytrm sadttlvekf egclvgallg dclgapfetd ftgdafpnqq
      121 tffqnllkht vvpgpsagrn frpsnvhpgh geyhytddta msmclansll akrkfdaldv
      181 akrftteyfe neaivfeygn avrdvfrklk nnnyedpykp aqeqfggrgs ygngaamrva
      241 pvalfymddv ekmvevarnq akithsnqtg ysaaimqcta vhlalksdpk ipldpvafid
      301 hlialmdkle eavdgfkplv fklmhikklv snpdldptpa evaktlgnni taqgsvptai
      361 ysflrsqrpl kdfehespfv rclyfavacg gdtdtiatma csisgafhgi gaipsvlqrq
      421 cqsvdevtri adyleeavsk
//
LOCUS       XP_077502109             419 aa            linear   INV 25-NOV-2025
DEFINITION  ADP-ribosylhydrolase ARH3-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502109
VERSION     XP_077502109.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645983.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..419
                     /product="ADP-ribosylhydrolase ARH3-like isoform X2"
                     /calculated_mol_wt=45557
     Region          65..416
                     /region_name="DraG"
                     /note="ADP-ribosylglycohydrolase [Posttranslational
                     modification, protein turnover, chaperones]; COG1397"
                     /db_xref="CDD:441007"
     CDS             1..419
                     /gene="LOC144113076"
                     /coded_by="XM_077645983.1:16..1275"
                     /db_xref="GeneID:144113076"
ORIGIN      
        1 mveysgsevd llvviqperr hprisctsvs fplppgpsas gvaasahass galpkytrms
       61 adttlvekfe gclvgallgd clgapfetdf tgdafpnqqt ffqnllkhtv vpgpsagrnf
      121 rpsnvhpghg eyhytddtam smclanslla krkfdaldva krftteyfen eaivfeygna
      181 vrdvfrklkn nnyedpykpa qeqfggrgsy gngaamrvap valfymddve kmvevarnqa
      241 kithsnqtgy saaimqctav hlalksdpki pldpvafidh lialmdklee avdgfkplvf
      301 klmhikklvs npdldptpae vaktlgnnit aqgsvptaiy sflrsqrplk dfehespfvr
      361 clyfavacgg dtdtiatmac sisgafhgig aipsvlqrqc qsvdevtria dyleeavsk
//
LOCUS       XP_077502110             380 aa            linear   INV 25-NOV-2025
DEFINITION  ADP-ribosylhydrolase ARH3-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077502110
VERSION     XP_077502110.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645984.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..380
                     /product="ADP-ribosylhydrolase ARH3-like isoform X3"
                     /calculated_mol_wt=41853
     Region          26..377
                     /region_name="DraG"
                     /note="ADP-ribosylglycohydrolase [Posttranslational
                     modification, protein turnover, chaperones]; COG1397"
                     /db_xref="CDD:441007"
     CDS             1..380
                     /gene="LOC144113076"
                     /coded_by="XM_077645984.1:134..1276"
                     /db_xref="GeneID:144113076"
ORIGIN      
        1 mpaprrsfvv rnllrqytrm sadttlvekf egclvgallg dclgapfetd ftgdafpnqq
       61 tffqnllkht vvpgpsagrn frpsnvhpgh geyhytddta msmclansll akrkfdaldv
      121 akrftteyfe neaivfeygn avrdvfrklk nnnyedpykp aqeqfggrgs ygngaamrva
      181 pvalfymddv ekmvevarnq akithsnqtg ysaaimqcta vhlalksdpk ipldpvafid
      241 hlialmdkle eavdgfkplv fklmhikklv snpdldptpa evaktlgnni taqgsvptai
      301 ysflrsqrpl kdfehespfv rclyfavacg gdtdtiatma csisgafhgi gaipsvlqrq
      361 cqsvdevtri adyleeavsk
//
LOCUS       XP_077502111             366 aa            linear   INV 25-NOV-2025
DEFINITION  ADP-ribosylhydrolase ARH3-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077502111
VERSION     XP_077502111.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645985.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..366
                     /product="ADP-ribosylhydrolase ARH3-like isoform X4"
                     /calculated_mol_wt=40149
     Region          12..363
                     /region_name="DraG"
                     /note="ADP-ribosylglycohydrolase [Posttranslational
                     modification, protein turnover, chaperones]; COG1397"
                     /db_xref="CDD:441007"
     CDS             1..366
                     /gene="LOC144113076"
                     /coded_by="XM_077645985.1:16..1116"
                     /db_xref="GeneID:144113076"
ORIGIN      
        1 msytrmsadt tlvekfegcl vgallgdclg apfetdftgd afpnqqtffq nllkhtvvpg
       61 psagrnfrps nvhpghgeyh ytddtamsmc lansllakrk fdaldvakrf tteyfeneai
      121 vfeygnavrd vfrklknnny edpykpaqeq fggrgsygng aamrvapval fymddvekmv
      181 evarnqakit hsnqtgysaa imqctavhla lksdpkipld pvafidhlia lmdkleeavd
      241 gfkplvfklm hikklvsnpd ldptpaevak tlgnnitaqg svptaiysfl rsqrplkdfe
      301 hespfvrcly favacggdtd tiatmacsis gafhgigaip svlqrqcqsv devtriadyl
      361 eeavsk
//
LOCUS       XP_077502112             361 aa            linear   INV 25-NOV-2025
DEFINITION  ADP-ribosylhydrolase ARH3-like isoform X5 [Amblyomma americanum].
ACCESSION   XP_077502112
VERSION     XP_077502112.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645986.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..361
                     /product="ADP-ribosylhydrolase ARH3-like isoform X5"
                     /calculated_mol_wt=39510
     Region          7..358
                     /region_name="DraG"
                     /note="ADP-ribosylglycohydrolase [Posttranslational
                     modification, protein turnover, chaperones]; COG1397"
                     /db_xref="CDD:441007"
     CDS             1..361
                     /gene="LOC144113076"
                     /coded_by="XM_077645986.1:165..1250"
                     /db_xref="GeneID:144113076"
ORIGIN      
        1 msadttlvek fegclvgall gdclgapfet dftgdafpnq qtffqnllkh tvvpgpsagr
       61 nfrpsnvhpg hgeyhytddt amsmclansl lakrkfdald vakrftteyf eneaivfeyg
      121 navrdvfrkl knnnyedpyk paqeqfggrg sygngaamrv apvalfymdd vekmvevarn
      181 qakithsnqt gysaaimqct avhlalksdp kipldpvafi dhlialmdkl eeavdgfkpl
      241 vfklmhikkl vsnpdldptp aevaktlgnn itaqgsvpta iysflrsqrp lkdfehespf
      301 vrclyfavac ggdtdtiatm acsisgafhg igaipsvlqr qcqsvdevtr iadyleeavs
      361 k
//
LOCUS       XP_077502113             361 aa            linear   INV 25-NOV-2025
DEFINITION  ADP-ribosylhydrolase ARH3-like isoform X5 [Amblyomma americanum].
ACCESSION   XP_077502113
VERSION     XP_077502113.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645987.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..361
                     /product="ADP-ribosylhydrolase ARH3-like isoform X5"
                     /calculated_mol_wt=39510
     Region          7..358
                     /region_name="DraG"
                     /note="ADP-ribosylglycohydrolase [Posttranslational
                     modification, protein turnover, chaperones]; COG1397"
                     /db_xref="CDD:441007"
     CDS             1..361
                     /gene="LOC144113076"
                     /coded_by="XM_077645987.1:215..1300"
                     /db_xref="GeneID:144113076"
ORIGIN      
        1 msadttlvek fegclvgall gdclgapfet dftgdafpnq qtffqnllkh tvvpgpsagr
       61 nfrpsnvhpg hgeyhytddt amsmclansl lakrkfdald vakrftteyf eneaivfeyg
      121 navrdvfrkl knnnyedpyk paqeqfggrg sygngaamrv apvalfymdd vekmvevarn
      181 qakithsnqt gysaaimqct avhlalksdp kipldpvafi dhlialmdkl eeavdgfkpl
      241 vfklmhikkl vsnpdldptp aevaktlgnn itaqgsvpta iysflrsqrp lkdfehespf
      301 vrclyfavac ggdtdtiatm acsisgafhg igaipsvlqr qcqsvdevtr iadyleeavs
      361 k
//
LOCUS       XP_077502114             361 aa            linear   INV 25-NOV-2025
DEFINITION  ADP-ribosylhydrolase ARH3-like isoform X5 [Amblyomma americanum].
ACCESSION   XP_077502114
VERSION     XP_077502114.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645988.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..361
                     /product="ADP-ribosylhydrolase ARH3-like isoform X5"
                     /calculated_mol_wt=39510
     Region          7..358
                     /region_name="DraG"
                     /note="ADP-ribosylglycohydrolase [Posttranslational
                     modification, protein turnover, chaperones]; COG1397"
                     /db_xref="CDD:441007"
     CDS             1..361
                     /gene="LOC144113076"
                     /coded_by="XM_077645988.1:115..1200"
                     /db_xref="GeneID:144113076"
ORIGIN      
        1 msadttlvek fegclvgall gdclgapfet dftgdafpnq qtffqnllkh tvvpgpsagr
       61 nfrpsnvhpg hgeyhytddt amsmclansl lakrkfdald vakrftteyf eneaivfeyg
      121 navrdvfrkl knnnyedpyk paqeqfggrg sygngaamrv apvalfymdd vekmvevarn
      181 qakithsnqt gysaaimqct avhlalksdp kipldpvafi dhlialmdkl eeavdgfkpl
      241 vfklmhikkl vsnpdldptp aevaktlgnn itaqgsvpta iysflrsqrp lkdfehespf
      301 vrclyfavac ggdtdtiatm acsisgafhg igaipsvlqr qcqsvdevtr iadyleeavs
      361 k
//
LOCUS       XP_077502115             634 aa            linear   INV 25-NOV-2025
DEFINITION  heat shock protein 70 B2-like [Amblyomma americanum].
ACCESSION   XP_077502115
VERSION     XP_077502115.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645989.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..634
                     /product="heat shock protein 70 B2-like"
                     /calculated_mol_wt=70025
     Region          2..634
                     /region_name="PTZ00009"
                     /note="heat shock 70 kDa protein; Provisional"
                     /db_xref="CDD:240227"
     CDS             1..634
                     /gene="LOC144113077"
                     /coded_by="XM_077645989.1:506..2410"
                     /db_xref="GeneID:144113077"
ORIGIN      
        1 msksapaigi dlgttfscvg vfqhgrveii andqgnrttp syvaftdter ligdpakaqv
       61 alnpentvfd akrligrkyd dpkiqqdihh wpfkivnqgg kpnicvefkg ekkvfnpeei
      121 samvltkmke taeaylgspv kdavvtvpay fndsqrqatk dagaiaglnv lriineptaa
      181 alaygldknl kgeknilifd lgggtfdvsi ltidegsmfe vrstagdthl ggedfdnrlv
      241 nyfveefkrk hkrdlktntr airrlrtace rakrtlssss easieidalf egidfyskit
      301 rarfeemcmd lfrstlepve kalqdsklsk sqihdivlvg gstripriqk llkdffngkd
      361 lamsinpdea vaygaaiqaa iltgdtseii rdvllvdvap lslgietagg vmakiverns
      421 ripcktsktf ttyadnqtgv tiqvfegera mtkdnhllgt fdlmgippap rgvpqiqvtf
      481 dldangilqv sakdestgrs esiritndkg rlskeeidrm ladaekyrqe dekqrervta
      541 rnnlesyvyn vkqsvemvgp ekldaadkqk vlskceevin widrntlaek eeyddkmkdm
      601 qqvctpimtk lhqgasqapp nqggaagpki devd
//
LOCUS       XP_077502116             232 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113078 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502116
VERSION     XP_077502116.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645990.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..232
                     /product="uncharacterized protein LOC144113078 isoform X1"
                     /calculated_mol_wt=25377
     CDS             1..232
                     /gene="LOC144113078"
                     /coded_by="XM_077645990.1:124..822"
                     /db_xref="GeneID:144113078"
ORIGIN      
        1 mhctwrkqlr acasackvrl qdstmrlavf aalcvgafga tvqdvnefvd tvlgdkmqtf
       61 pnldpmnalg ryvklgnvir sdwtanlknv qvtglskvrr sgdcaeakal psgrvvvqcs
      121 lsmdalvvqf tseqsylggr pdvigvtatf pphlvslrae vgarrypdvk ltlrskleps
      181 lsytnlgklt enramkngys yaasdtlhrq lqkeyvdyla qavmgvplpy gr
//
LOCUS       XP_077502117             208 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113078 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502117
VERSION     XP_077502117.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645991.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..208
                     /product="uncharacterized protein LOC144113078 isoform X2"
                     /calculated_mol_wt=22602
     CDS             1..208
                     /gene="LOC144113078"
                     /coded_by="XM_077645991.1:121..747"
                     /db_xref="GeneID:144113078"
ORIGIN      
        1 mrlavfaalc vgafgatvqd vnefvdtvlg dkmqtfpnld pmnalgryvk lgnvirsdwt
       61 anlknvqvtg lskvrrsgdc aeakalpsgr vvvqcslsmd alvvqftseq sylggrpdvi
      121 gvtatfpphl vslraevgar rypdvkltlr sklepslsyt nlgkltenra mkngysyaas
      181 dtlhrqlqke yvdylaqavm gvplpygr
//
LOCUS       XP_077502118             207 aa            linear   INV 25-NOV-2025
DEFINITION  salivary anticoagulant protein P23-like [Amblyomma americanum].
ACCESSION   XP_077502118
VERSION     XP_077502118.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645992.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..207
                     /product="salivary anticoagulant protein P23-like"
                     /calculated_mol_wt=22555
     CDS             1..207
                     /gene="LOC144113079"
                     /coded_by="XM_077645992.1:41..664"
                     /db_xref="GeneID:144113079"
ORIGIN      
        1 mlpavlvtlf vgafgatvyd anryidevlg rrmpvlvsrn rldplpvady itnlgapnvl
       61 saqmrkvnvt glglvrrqgd cgrpgtsgpg rvtvgcnvvl drvavsatsd lryymepyqr
      121 igvranfdtn halvevtsvv gqppivnfrl lrplvprltf snlknlplys viekgysqel
      181 srilqtqlsg pylaslgtac rdvpfpr
//
LOCUS       XP_077502119             140 aa            linear   INV 25-NOV-2025
DEFINITION  placenta growth factor-like [Amblyomma americanum].
ACCESSION   XP_077502119
VERSION     XP_077502119.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645993.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..140
                     /product="placenta growth factor-like"
                     /calculated_mol_wt=15595
     Region          44..135
                     /region_name="PDGF"
                     /note="Platelet-derived and vascular endothelial growth
                     factors (PDGF, VEGF) family domain; PDGF is a potent
                     activator for cells of mesenchymal origin; PDGF-A and
                     PDGF-B form AA and BB homodimers and an AB heterodimer;
                     VEGF is a potent mitogen in embryonic and...; cl00116"
                     /db_xref="CDD:444701"
     Site            order(44..47,84,86..89,104..106,110)
                     /site_type="active"
                     /note="receptor binding interface [active]"
                     /db_xref="CDD:238079"
     Site            order(46,77,84,91,130,132)
                     /site_type="other"
                     /note="cysteine knot motif"
                     /db_xref="CDD:238079"
     Site            order(71,83)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:238079"
     CDS             1..140
                     /gene="LOC144113080"
                     /coded_by="XM_077645993.1:647..1069"
                     /db_xref="GeneID:144113080"
ORIGIN      
        1 mrrlaltvlv lsavlcraap aprrpanpas araarhqefv wreaacrvpq prvqclkelq
       61 pndtrkfvph ctilhrcapd tgccaaeeqh cqvktvqavq lpflvlhldt gggpsryepv
      121 tlvfdnhtec ecrlrnepir
//
LOCUS       XP_077502120             149 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113081 [Amblyomma americanum].
ACCESSION   XP_077502120
VERSION     XP_077502120.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645994.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..149
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..149
                     /product="uncharacterized protein LOC144113081"
                     /calculated_mol_wt=16849
     Region          60..144
                     /region_name="PDGF"
                     /note="Platelet-derived and vascular endothelial growth
                     factors (PDGF, VEGF) family domain; PDGF is a potent
                     activator for cells of mesenchymal origin; PDGF-A and
                     PDGF-B form AA and BB homodimers and an AB heterodimer;
                     VEGF is a potent mitogen in embryonic and...; cl00116"
                     /db_xref="CDD:444701"
     Site            order(81,93)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:238079"
     Site            order(87,94,101,139,141)
                     /site_type="other"
                     /note="cysteine knot motif"
                     /db_xref="CDD:238079"
     CDS             1..149
                     /gene="LOC144113081"
                     /coded_by="XM_077645994.1:129..578"
                     /db_xref="GeneID:144113081"
ORIGIN      
        1 masehervar glawtallvl ctcfavglcr phkpygsray vrasqhlqmv qrearcrvpq
       61 prticvpdiy pneskryvph ctvvhrcgpd tgccatedeh cqpraiqvve rtflvlelea
      121 sgeqqtkves lafenhtece crakydhir
//
LOCUS       XP_077502121             255 aa            linear   INV 25-NOV-2025
DEFINITION  D-ribitol-5-phosphate cytidylyltransferase-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077502121
VERSION     XP_077502121.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645995.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..255
                     /product="D-ribitol-5-phosphate cytidylyltransferase-like
                     isoform X1"
                     /calculated_mol_wt=27976
     Region          14..193
                     /region_name="CDP-ME_synthetase"
                     /note="CDP-ME synthetase is involved in
                     mevalonate-independent isoprenoid production; cd02516"
                     /db_xref="CDD:133009"
     Site            order(19,21..26,33,91..94,97,117..119)
                     /site_type="active"
                     /note="substrate binding site [active]"
                     /db_xref="CDD:133009"
     CDS             1..255
                     /gene="LOC144113082"
                     /coded_by="XM_077645995.1:171..938"
                     /db_xref="GeneID:144113082"
ORIGIN      
        1 mdrsekpfmm dsivsvilpa agtgermkik mpkqfcevqg kpvichtiga flqyswisei
       61 vvvyppdkys lvvstlntad llekvtlvpg ggtrhrsimn gmkhleqdpp diviihdavr
      121 pivpkhvlee vvqeaklhgg agpvvplvst vlkiddkgfl eesldrsryv asetpqafqy
      181 pllmkayqrs teedytngpw essyhsfhdg aavkgcatal rwqvlpavgl vrprllfpsn
      241 lvqstinlaa tcqgg
//
LOCUS       XP_077502122             220 aa            linear   INV 25-NOV-2025
DEFINITION  D-ribitol-5-phosphate cytidylyltransferase-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077502122
VERSION     XP_077502122.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645996.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..220
                     /product="D-ribitol-5-phosphate cytidylyltransferase-like
                     isoform X2"
                     /calculated_mol_wt=24474
     Region          14..193
                     /region_name="CDP-ME_synthetase"
                     /note="CDP-ME synthetase is involved in
                     mevalonate-independent isoprenoid production; cd02516"
                     /db_xref="CDD:133009"
     Site            order(19,21..26,33,91..94,97,117..119)
                     /site_type="active"
                     /note="substrate binding site [active]"
                     /db_xref="CDD:133009"
     CDS             1..220
                     /gene="LOC144113082"
                     /coded_by="XM_077645996.1:170..832"
                     /db_xref="GeneID:144113082"
ORIGIN      
        1 mdrsekpfmm dsivsvilpa agtgermkik mpkqfcevqg kpvichtiga flqyswisei
       61 vvvyppdkys lvvstlntad llekvtlvpg ggtrhrsimn gmkhleqdpp diviihdavr
      121 pivpkhvlee vvqeaklhgg agpvvplvst vlkiddkgfl eesldrsryv asetpqafqy
      181 pllmkayqrs teedytnvlc tlpmiclsyd kpkyfsliaq
//
LOCUS       XP_077502123            1950 aa            linear   INV 25-NOV-2025
DEFINITION  dedicator of cytokinesis protein myoblast city [Amblyomma
            americanum].
ACCESSION   XP_077502123
VERSION     XP_077502123.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645997.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1950
                     /product="dedicator of cytokinesis protein myoblast city"
                     /calculated_mol_wt=223038
     Region          13..68
                     /region_name="SH3_DOCK_AB"
                     /note="Src Homology 3 domain of Class A and B Dedicator of
                     Cytokinesis proteins; cd11872"
                     /db_xref="CDD:212805"
     Site            order(15..19,26,31..33,35,39..40,44..45,47,55,57,59,61,
                     63..64,66,68)
                     /site_type="other"
                     /note="ELMO interaction site [polypeptide binding]"
                     /db_xref="CDD:212805"
     Site            order(18,20,23,27,44..45,61,63..64)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212805"
     Region          76..420
                     /region_name="DOCK_N"
                     /note="DOCK N-terminus; pfam16172"
                     /db_xref="CDD:465040"
     Region          428..634
                     /region_name="C2_Dock-A"
                     /note="C2 domains found in Dedicator Of CytoKinesis (Dock)
                     class A proteins; cd08694"
                     /db_xref="CDD:176076"
     Region          1248..1647
                     /region_name="DHR2_DOCK_A"
                     /note="Dock Homology Region 2, a GEF domain, of Class A
                     Dedicator of Cytokinesis proteins; cd11697"
                     /db_xref="CDD:212570"
     Site            order(1332,1339..1341,1343,1345..1346,1348..1349,
                     1352..1353,1355..1356,1359..1360,1363,1450,1454..1457,
                     1460)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:212570"
     Site            order(1398,1400..1403,1419..1421,1438..1440,1474..1477,
                     1488..1489,1492,1531,1535,1555..1556,1558..1559,
                     1563..1564,1567..1573,1576,1606,1622,1624..1625,1628,1631)
                     /site_type="other"
                     /note="Rac binding site [polypeptide binding]"
                     /db_xref="CDD:212570"
     Site            1567..1572
                     /site_type="other"
                     /note="nucleotide sensor"
                     /db_xref="CDD:212570"
     Region          <1670..1927
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..1950
                     /gene="mbc"
                     /coded_by="XM_077645997.1:185..6037"
                     /db_xref="GeneID:144113083"
ORIGIN      
        1 mtkwtpvsdk akygvaianf teegvhrlrl nvgdsvyiye eledwyygcc snsktkkgif
       61 pkayiavkds iidktgphev vipkepsiik eitavlrewg piwkqlylts raefdsvrnm
      121 myelmdsrrk imsgtlpvde lrelkqkvta kiemgnaild ldlvvrhssg nilnpdvtst
      181 idlyrahvda tkrikqmssa qldigspkas shychnlfvv vknfvcrigd dadllmtlyd
      241 akeqkfisen yyvqwakcgl ikdldqlnnl rvlftdlgsr dltrervyfi cqiirigame
      301 lkenehkrst lyskkvpese gmrrpfgvaa mditdvisgk idsdeekqhf vpyvqcgerd
      361 fldtaikkal aakevtqkeh kgqglwiclk llhgdnkqvr eenphlvsgt tavarkmgfp
      421 evilpgdvrn dlyltlvqae fskgaktsdr nievtvkvcn dqgvvipgvi sigsgsesld
      481 eyhsviyyhe dkprwmetfk vavpieafyk ahllftfkhr ssneakdrve kpfamsfvkl
      541 mqengttlnd evhellvyrl dhkrhvemdt syltlpatra efnsimqnss tpnaqpnkaq
      601 lasfqtggis laikdsfqis tlvcstkltq nvdllgllkw wtlpdnlekn lhalmkvdge
      661 evvkflqdil dalfdilmkn sdselydnlv fealvfiigl isdrkyqhfk pvldlyieet
      721 fsatlaynkl ivvlkyyidn intntqeteg sllframksi eyifkfivhs rklfaslneg
      781 kgtqqfelsl eqllksitgm mlykadtvll iqgaclkyfp atipdilhvf naeqlsyivt
      841 ellnnlpper ltkqkmmcvn divhsevfkf pecraillpv inqhvqllme kgeelelcvk
      901 ilsdimvvlh ghdrtqtied isvvmhsvlr tviqtvirtd rsspivgniv almiailrqm
      961 akchyhsyld hfptstdlld flmeillvfk dlvsknvypk dwndmimlqn svmlkalrhf
     1021 sytirdrftn pfdyqvwnny fhcaiafltq dalqlenfsh nkrnkiiary kdmrretgfe
     1081 irkmwfnlgq nkirfvpgmv gpflemtmip eaelrkatip iffdmmqcef ysprkavsyh
     1141 kddcyvhnle ikgnfqefen emitkldvlv eggrgdqqyq dflkqilfsl cenhatlrda
     1201 cekfintvve lmkrlleyrt iitdenkenr msctvnlldf yheisrqemy irylhklcdl
     1261 hlecdnyiea aftlqlhakl lrwsdeslps llrsakypqc etnrelkerl yhdildyfdk
     1321 gklweagvtl skellsqyen etfdyaqlss lhariatfye nimrqlrpep eyfrvayygr
     1381 nfpaflqnkv fvyrgkeyer lsdfssrlln qfpnallltk ltipgkdvte sqaqylqink
     1441 vdpvmnnphr fqnkpvheqi lkyyrvnevn kftysrplrr gekdsdsdig niwlerttle
     1501 tahklpgilr wfvvvkaqtv evsplqnaie tmeatntkir dmvlqyradt slplnplsml
     1561 lggivdaavn ggignyekaf ftqefleanr satdginrlk eliacqipll eagieihrdk
     1621 apeslqpyhs hmqekylklk ahieetygkv ppppelnehp ilirrykvvp pstqssrter
     1681 tdssvfapes snrktasiss lpgvpgkpqs tqltralvps vfvkqnsvvs phkphgkksr
     1741 dstgltlrrn saaslssqhe eksqsqwydl plttekgsea piielneqlt phrplrseae
     1801 krhsrpssgq fklppstpps qagsggattp slatamsfpm fpassigpac speppqevdr
     1861 ppplpqkqay adytnvtedt pvlparkpsl sipgmtknrp qpplpakegs kegtpvlpkk
     1921 perpptnrnl sfpqdvtkkm trtaseateg
//
LOCUS       XP_077502124             860 aa            linear   INV 25-NOV-2025
DEFINITION  kelch-like protein 26 [Amblyomma americanum].
ACCESSION   XP_077502124
VERSION     XP_077502124.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645998.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..860
                     /product="kelch-like protein 26"
                     /calculated_mol_wt=94658
     Region          1..698
                     /region_name="PHA03098"
                     /note="kelch-like protein; Provisional"
                     /db_xref="CDD:222983"
     Region          127..211
                     /region_name="BACK"
                     /note="BTB And C-terminal Kelch; smart00875"
                     /db_xref="CDD:197943"
     Region          530..799
                     /region_name="NanM"
                     /note="N-acetylneuraminic acid mutarotase [Cell
                     wall/membrane/envelope biogenesis]; COG3055"
                     /db_xref="CDD:442289"
     Region          541..591
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          595..641
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          751..799
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     CDS             1..860
                     /gene="LOC144113084"
                     /coded_by="XM_077645998.1:194..2776"
                     /db_xref="GeneID:144113084"
ORIGIN      
        1 matssppadg dliemivdde vlsfskallm qsspffqhtf tdgqpkkkrv visgvsgqtf
       61 khllgymksg klelscnnia difhaacklq mpdiirqcie mearssavgr qiilykaakr
      121 vnnveemnaa lyrmvsnftl vtqsdeflel dakdvcells cdvlscerel dvfnaarrwl
      181 nhkvdtrlal tsrlmycvrf plmslmdlsh cveadtppgl qddhnvrvmi lsavcfwvak
      241 aagkeaevgh lscmprhyik kdaevstdgm etamtcgrse qptllsvtlg aaktrllpaa
      301 sskieegtqe lhiasvlesg hlnvpehsie qeaslsllss taecdcsmds vaptvcessv
      361 snifssevqa tcpstqesdg asvtqdtqct yspsdfcsrs dddkdqlssq svdsvilhgf
      421 sggdivhrdy vsnlktklll ssqrgvehev lfarspllse qpkadflvvs dnpensaips
      481 asvsagvehn fsdtpckdge qgtlfliggi eadespsmcp ilsysikgns wsprktlpvh
      541 ryghkavfld gciyiiggfe nfspvnleka ssrccfwfev ssgkwgimap lnktrahhgl
      601 avldntiyai ggvdsnnkll gclerydkek dewtklsael ysprmamgfc shkrqlwvag
      661 givqigrrtc stayvevynp eterwafavn flpsprsclt lvecggtmya vggllshrcg
      721 rkrifttved tlafvderkt wvhrapmpna rhttvaaahd gvifiiggrq aerpdyhfdn
      781 ilaydtkldk wmtlgnlptn vtdyhcvlap pekpsdnlre lltsrgdhrs ptkmadrfpv
      841 aapdvksysq gdiavqqqnk
//
LOCUS       XP_077502125             882 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113085 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502125
VERSION     XP_077502125.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077645999.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..882
                     /product="uncharacterized protein LOC144113085 isoform X1"
                     /calculated_mol_wt=97668
     Region          3..51
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(3,8,23,26,31,34)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     Region          96..149
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(104,109,124,127,132,135)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     Region          <439..465
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Region          <584..610
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Region          645..695
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(653,656,671,673,676,679,694)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          732..797
                     /region_name="BRcat_RBR_HOIL1"
                     /note="BRcat domain found in heme-oxidized IRP2 ubiquitin
                     ligase 1 (HOIL-1) and similar proteins; cd20345"
                     /db_xref="CDD:439006"
     Region          810..882
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(819,822,837,840,845,865)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     CDS             1..882
                     /gene="LOC144113085"
                     /coded_by="XM_077645999.1:145..2793"
                     /db_xref="GeneID:144113085"
ORIGIN      
        1 mdckrigcsg rvqpngtvpf avcqecrspn clrcsaihen qsckkyqnsi qykgspgkia
       61 aqnntafspp rvpqldkspm qascpaappa vaiddnssek lvqckvvgcd grafvdktda
      121 lfrcplcnhy tcinceavhe smtcaqyren kdsiaapvhk prrqseedse eaevekpeqp
      181 aadddaegmi vdccyegcsg yayvfktatv akcelcehwt cvsckaches qscavykgid
      241 vpeecaaqep tvsenqrrgr nreqksvsag stehggflsg vlknfgklls gsgsgtvees
      301 paqmplasss adnyrshpgr vpcarghtef ekvqgngipp cdacssrksd htaeecqhal
      361 crqciqksve katvyrsvpc papagnsqpc tghlreeevq qvvprnilrn lfkksdwrds
      421 qcfdkrcsha frrkkgaqff tcpmcrrknc adclaihegm tceqyiekfv admdrgkleg
      481 ngippcdacs srksdhtaee cqhalcrqci qksvekatvy rsvpcpapag nsqpctghlr
      541 eeevqqvvpr nilrnlfkks dwrdsqcfdk rcshafrrkk gaqfftcpmc rrkncadcla
      601 ihegmtceqy iekfvadmdr gklenpeekk krlqeqialy erpsiqttee fecpiclvev
      661 epgmgivlkn chhqiceqcl adtaknsqaa evlcpcksdd adcqmpvlds dlraclsead
      721 yaalqerglr eaenaskeps fhcktpdckg wclhgrevev fdcpvcqrqn clrceaihkd
      781 mscadyqadl krraendvaa raslnfleem lkteqamrcp qcrvviikrs gcdfmvctsc
      841 ktqlcwatkg arwgpggqgd tsggcrcgvg gvkchpkctt ch
//
LOCUS       XP_077502126             853 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113085 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502126
VERSION     XP_077502126.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646000.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..853
                     /product="uncharacterized protein LOC144113085 isoform X2"
                     /calculated_mol_wt=94665
     Region          3..51
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(3,8,23,26,31,34)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     Region          67..120
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(75,80,95,98,103,106)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     Region          <410..436
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Region          <555..581
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Region          616..666
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(624,627,642,644,647,650,665)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          703..768
                     /region_name="BRcat_RBR_HOIL1"
                     /note="BRcat domain found in heme-oxidized IRP2 ubiquitin
                     ligase 1 (HOIL-1) and similar proteins; cd20345"
                     /db_xref="CDD:439006"
     Region          781..853
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(790,793,808,811,816,836)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     CDS             1..853
                     /gene="LOC144113085"
                     /coded_by="XM_077646000.1:145..2706"
                     /db_xref="GeneID:144113085"
ORIGIN      
        1 mdckrigcsg rvqpngtvpf avcqecrspn clrcsaihen qsckkyqnsi qykgspgkia
       61 avaiddnsse klvqckvvgc dgrafvdktd alfrcplcnh ytcinceavh esmtcaqyre
      121 nkdsiaapvh kprrqseeds eeaevekpeq paadddaegm ivdccyegcs gyayvfktat
      181 vakcelcehw tcvsckache sqscavykgi dvpeecaaqe ptvsenqrrg rnreqksvsa
      241 gstehggfls gvlknfgkll sgsgsgtvee spaqmplass sadnyrshpg rvpcarghte
      301 fekvqgngip pcdacssrks dhtaeecqha lcrqciqksv ekatvyrsvp cpapagnsqp
      361 ctghlreeev qqvvprnilr nlfkksdwrd sqcfdkrcsh afrrkkgaqf ftcpmcrrkn
      421 cadclaiheg mtceqyiekf vadmdrgkle gngippcdac ssrksdhtae ecqhalcrqc
      481 iqksvekatv yrsvpcpapa gnsqpctghl reeevqqvvp rnilrnlfkk sdwrdsqcfd
      541 krcshafrrk kgaqfftcpm crrkncadcl aihegmtceq yiekfvadmd rgklenpeek
      601 kkrlqeqial yerpsiqtte efecpiclve vepgmgivlk nchhqiceqc ladtaknsqa
      661 aevlcpcksd dadcqmpvld sdlraclsea dyaalqergl reaenaskep sfhcktpdck
      721 gwclhgreve vfdcpvcqrq nclrceaihk dmscadyqad lkrraendva araslnflee
      781 mlkteqamrc pqcrvviikr sgcdfmvcts cktqlcwatk garwgpggqg dtsggcrcgv
      841 ggvkchpkct tch
//
LOCUS       XP_077502127             842 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113085 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502127
VERSION     XP_077502127.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646001.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..842
                     /product="uncharacterized protein LOC144113085 isoform X3"
                     /calculated_mol_wt=93398
     Region          3..51
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(3,8,23,26,31,34)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     Region          96..149
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(104,109,124,127,132,135)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     Region          <399..425
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Region          <544..570
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Region          605..655
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(613,616,631,633,636,639,654)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          692..757
                     /region_name="BRcat_RBR_HOIL1"
                     /note="BRcat domain found in heme-oxidized IRP2 ubiquitin
                     ligase 1 (HOIL-1) and similar proteins; cd20345"
                     /db_xref="CDD:439006"
     Region          770..842
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(779,782,797,800,805,825)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     CDS             1..842
                     /gene="LOC144113085"
                     /coded_by="XM_077646001.1:250..2778"
                     /db_xref="GeneID:144113085"
ORIGIN      
        1 mdckrigcsg rvqpngtvpf avcqecrspn clrcsaihen qsckkyqnsi qykgspgkia
       61 aqnntafspp rvpqldkspm qascpaappa vaiddnssek lvqckvvgcd grafvdktda
      121 lfrcplcnhy tcinceavhe smtcaqyren kdsiaapvhk prrqseedse eaevekpeqp
      181 aadddaegmi vdccyegcsg yayvfktatv akcelcehwt cvsckaches qscavykgid
      241 vpeecaaqep tsgsgtvees paqmplasss adnyrshpgr vpcarghtef ekvqgngipp
      301 cdacssrksd htaeecqhal crqciqksve katvyrsvpc papagnsqpc tghlreeevq
      361 qvvprnilrn lfkksdwrds qcfdkrcsha frrkkgaqff tcpmcrrknc adclaihegm
      421 tceqyiekfv admdrgkleg ngippcdacs srksdhtaee cqhalcrqci qksvekatvy
      481 rsvpcpapag nsqpctghlr eeevqqvvpr nilrnlfkks dwrdsqcfdk rcshafrrkk
      541 gaqfftcpmc rrkncadcla ihegmtceqy iekfvadmdr gklenpeekk krlqeqialy
      601 erpsiqttee fecpiclvev epgmgivlkn chhqiceqcl adtaknsqaa evlcpcksdd
      661 adcqmpvlds dlraclsead yaalqerglr eaenaskeps fhcktpdckg wclhgrevev
      721 fdcpvcqrqn clrceaihkd mscadyqadl krraendvaa raslnfleem lkteqamrcp
      781 qcrvviikrs gcdfmvctsc ktqlcwatkg arwgpggqgd tsggcrcgvg gvkchpkctt
      841 ch
//
LOCUS       XP_077502128             813 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113085 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077502128
VERSION     XP_077502128.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646002.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..813
                     /product="uncharacterized protein LOC144113085 isoform X4"
                     /calculated_mol_wt=90394
     Region          3..51
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(3,8,23,26,31,34)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     Region          67..120
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(75,80,95,98,103,106)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     Region          <370..396
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Region          <515..541
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Region          576..626
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(584,587,602,604,607,610,625)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          663..728
                     /region_name="BRcat_RBR_HOIL1"
                     /note="BRcat domain found in heme-oxidized IRP2 ubiquitin
                     ligase 1 (HOIL-1) and similar proteins; cd20345"
                     /db_xref="CDD:439006"
     Region          741..813
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(750,753,768,771,776,796)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     CDS             1..813
                     /gene="LOC144113085"
                     /coded_by="XM_077646002.1:145..2586"
                     /db_xref="GeneID:144113085"
ORIGIN      
        1 mdckrigcsg rvqpngtvpf avcqecrspn clrcsaihen qsckkyqnsi qykgspgkia
       61 avaiddnsse klvqckvvgc dgrafvdktd alfrcplcnh ytcinceavh esmtcaqyre
      121 nkdsiaapvh kprrqseeds eeaevekpeq paadddaegm ivdccyegcs gyayvfktat
      181 vakcelcehw tcvsckache sqscavykgi dvpeecaaqe ptsgsgtvee spaqmplass
      241 sadnyrshpg rvpcarghte fekvqgngip pcdacssrks dhtaeecqha lcrqciqksv
      301 ekatvyrsvp cpapagnsqp ctghlreeev qqvvprnilr nlfkksdwrd sqcfdkrcsh
      361 afrrkkgaqf ftcpmcrrkn cadclaiheg mtceqyiekf vadmdrgkle gngippcdac
      421 ssrksdhtae ecqhalcrqc iqksvekatv yrsvpcpapa gnsqpctghl reeevqqvvp
      481 rnilrnlfkk sdwrdsqcfd krcshafrrk kgaqfftcpm crrkncadcl aihegmtceq
      541 yiekfvadmd rgklenpeek kkrlqeqial yerpsiqtte efecpiclve vepgmgivlk
      601 nchhqiceqc ladtaknsqa aevlcpcksd dadcqmpvld sdlraclsea dyaalqergl
      661 reaenaskep sfhcktpdck gwclhgreve vfdcpvcqrq nclrceaihk dmscadyqad
      721 lkrraendva araslnflee mlkteqamrc pqcrvviikr sgcdfmvcts cktqlcwatk
      781 garwgpggqg dtsggcrcgv ggvkchpkct tch
//
LOCUS       XP_077502129             751 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113086 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502129
VERSION     XP_077502129.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646003.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..751
                     /product="uncharacterized protein LOC144113086 isoform X1"
                     /calculated_mol_wt=82297
     Region          3..51
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(3,8,23,26,31,34)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     Region          104..149
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(104,109,124,127,132,135)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     Region          192..235
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(192,197,212,215,220,223)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     Region          <453..479
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Region          514..564
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(522,525,540,542,545,548,563)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          601..666
                     /region_name="BRcat_RBR_HOIL1"
                     /note="BRcat domain found in heme-oxidized IRP2 ubiquitin
                     ligase 1 (HOIL-1) and similar proteins; cd20345"
                     /db_xref="CDD:439006"
     Region          679..751
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(688,691,706,709,714,734)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     CDS             1..751
                     /gene="LOC144113086"
                     /coded_by="XM_077646003.1:145..2400"
                     /db_xref="GeneID:144113086"
ORIGIN      
        1 mdckrvgcsg rvqpngdvpf avcpecrspn clrcsaihed qscrqyqnsi rpkgspgkia
       61 aqnstacssp rsagsdkspr qapaptappa vaidddssek lvqckvvgcd grafvdktda
      121 lfrcplcdhy tcinceavhe smtcaqyren kdsvappvyk priqseednd eeddeeeqpa
      181 adddaegmiv eccnegcrgy ayvfktatva kcelcehwtc vsckachesq scaqykginv
      241 peecaapept asssyhgrgl svpsprwvsd nqrrgrnreq kavsagstep ggllsgvfkn
      301 fgkllsgagt aeelpaqmph gagpadnyrf dpgtvsgarg hteskkvqgr eilpcdacgy
      361 prsdyvvaec ehalcsqciq ksvekatshg svpcpalngi lqpcaghihe eevqqvvppe
      421 ilkklvkksn scdtkcfdkr cshafrrkkg aqfftcpmcr rkncadchai hegmtceqyi
      481 ekfvadmdrg kledpeekkk rleelmalye qdslptteef ecpiclveve pgkgivlrnc
      541 hhqiceqcla dtaknspaae vlcpckcdda dcqmsvldsd lraclseady aalqerglre
      601 aentskepsf hcktpdckgw clhgrevevf ycpicqqrnc lrceaihkdm rcadyqadlk
      661 rraendaaar aslnfleeml rtgqamrcpq crvviikreg cdgmvciscn tqlcwatkga
      721 rwgpggqgdi sggcrcgvgg vrchprcnyc h
//
LOCUS       XP_077502130             734 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113086 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502130
VERSION     XP_077502130.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646004.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..734
                     /product="uncharacterized protein LOC144113086 isoform X2"
                     /calculated_mol_wt=80471
     Region          3..51
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(3,8,23,26,31,34)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     Region          104..149
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(104,109,124,127,132,135)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     Region          192..235
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(192,197,212,215,220,223)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     Region          <436..462
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Region          497..547
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(505,508,523,525,528,531,546)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          584..649
                     /region_name="BRcat_RBR_HOIL1"
                     /note="BRcat domain found in heme-oxidized IRP2 ubiquitin
                     ligase 1 (HOIL-1) and similar proteins; cd20345"
                     /db_xref="CDD:439006"
     Region          662..734
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(671,674,689,692,697,717)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     CDS             1..734
                     /gene="LOC144113086"
                     /coded_by="XM_077646004.1:145..2349"
                     /db_xref="GeneID:144113086"
ORIGIN      
        1 mdckrvgcsg rvqpngdvpf avcpecrspn clrcsaihed qscrqyqnsi rpkgspgkia
       61 aqnstacssp rsagsdkspr qapaptappa vaidddssek lvqckvvgcd grafvdktda
      121 lfrcplcdhy tcinceavhe smtcaqyren kdsvappvyk priqseednd eeddeeeqpa
      181 adddaegmiv eccnegcrgy ayvfktatva kcelcehwtc vsckachesq scaqykginv
      241 peecaapept vsdnqrrgrn reqkavsags tepggllsgv fknfgkllsg agtaeelpaq
      301 mphgagpadn yrfdpgtvsg arghteskkv qgreilpcda cgyprsdyvv aecehalcsq
      361 ciqksvekat shgsvpcpal ngilqpcagh iheeevqqvv ppeilkklvk ksnscdtkcf
      421 dkrcshafrr kkgaqfftcp mcrrkncadc haihegmtce qyiekfvadm drgkledpee
      481 kkkrleelma lyeqdslptt eefecpiclv evepgkgivl rnchhqiceq cladtaknsp
      541 aaevlcpckc ddadcqmsvl dsdlraclse adyaalqerg lreaentske psfhcktpdc
      601 kgwclhgrev evfycpicqq rnclrceaih kdmrcadyqa dlkrraenda aaraslnfle
      661 emlrtgqamr cpqcrvviik regcdgmvci scntqlcwat kgarwgpggq gdisggcrcg
      721 vggvrchprc nych
//
LOCUS       XP_077502131             711 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113086 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502131
VERSION     XP_077502131.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646005.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..711
                     /product="uncharacterized protein LOC144113086 isoform X3"
                     /calculated_mol_wt=78096
     Region          3..51
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(3,8,23,26,31,34)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     Region          104..149
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(104,109,124,127,132,135)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     Region          192..235
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(192,197,212,215,220,223)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     Region          <413..439
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Region          474..524
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(482,485,500,502,505,508,523)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          561..626
                     /region_name="BRcat_RBR_HOIL1"
                     /note="BRcat domain found in heme-oxidized IRP2 ubiquitin
                     ligase 1 (HOIL-1) and similar proteins; cd20345"
                     /db_xref="CDD:439006"
     Region          639..711
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(648,651,666,669,674,694)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     CDS             1..711
                     /gene="LOC144113086"
                     /coded_by="XM_077646005.1:145..2280"
                     /db_xref="GeneID:144113086"
ORIGIN      
        1 mdckrvgcsg rvqpngdvpf avcpecrspn clrcsaihed qscrqyqnsi rpkgspgkia
       61 aqnstacssp rsagsdkspr qapaptappa vaidddssek lvqckvvgcd grafvdktda
      121 lfrcplcdhy tcinceavhe smtcaqyren kdsvappvyk priqseednd eeddeeeqpa
      181 adddaegmiv eccnegcrgy ayvfktatva kcelcehwtc vsckachesq scaqykginv
      241 peecaapept asssyhgrgl svpsprwagt aeelpaqmph gagpadnyrf dpgtvsgarg
      301 hteskkvqgr eilpcdacgy prsdyvvaec ehalcsqciq ksvekatshg svpcpalngi
      361 lqpcaghihe eevqqvvppe ilkklvkksn scdtkcfdkr cshafrrkkg aqfftcpmcr
      421 rkncadchai hegmtceqyi ekfvadmdrg kledpeekkk rleelmalye qdslptteef
      481 ecpiclveve pgkgivlrnc hhqiceqcla dtaknspaae vlcpckcdda dcqmsvldsd
      541 lraclseady aalqerglre aentskepsf hcktpdckgw clhgrevevf ycpicqqrnc
      601 lrceaihkdm rcadyqadlk rraendaaar aslnfleeml rtgqamrcpq crvviikreg
      661 cdgmvciscn tqlcwatkga rwgpggqgdi sggcrcgvgg vrchprcnyc h
//
LOCUS       XP_077502132             703 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113086 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077502132
VERSION     XP_077502132.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646006.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..703
                     /product="uncharacterized protein LOC144113086 isoform X4"
                     /calculated_mol_wt=76999
     Region          3..51
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(3,8,23,26,31,34)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     Region          104..149
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(104,109,124,127,132,135)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     Region          192..235
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(192,197,212,215,220,223)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     Region          <453..479
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Region          514..564
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(522,525,540,542,545,548,563)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          601..666
                     /region_name="BRcat_RBR_HOIL1"
                     /note="BRcat domain found in heme-oxidized IRP2 ubiquitin
                     ligase 1 (HOIL-1) and similar proteins; cd20345"
                     /db_xref="CDD:439006"
     CDS             1..703
                     /gene="LOC144113086"
                     /coded_by="XM_077646006.1:145..2256"
                     /db_xref="GeneID:144113086"
ORIGIN      
        1 mdckrvgcsg rvqpngdvpf avcpecrspn clrcsaihed qscrqyqnsi rpkgspgkia
       61 aqnstacssp rsagsdkspr qapaptappa vaidddssek lvqckvvgcd grafvdktda
      121 lfrcplcdhy tcinceavhe smtcaqyren kdsvappvyk priqseednd eeddeeeqpa
      181 adddaegmiv eccnegcrgy ayvfktatva kcelcehwtc vsckachesq scaqykginv
      241 peecaapept asssyhgrgl svpsprwvsd nqrrgrnreq kavsagstep ggllsgvfkn
      301 fgkllsgagt aeelpaqmph gagpadnyrf dpgtvsgarg hteskkvqgr eilpcdacgy
      361 prsdyvvaec ehalcsqciq ksvekatshg svpcpalngi lqpcaghihe eevqqvvppe
      421 ilkklvkksn scdtkcfdkr cshafrrkkg aqfftcpmcr rkncadchai hegmtceqyi
      481 ekfvadmdrg kledpeekkk rleelmalye qdslptteef ecpiclveve pgkgivlrnc
      541 hhqiceqcla dtaknspaae vlcpckcdda dcqmsvldsd lraclseady aalqerglre
      601 aentskepsf hcktpdckgw clhgrevevf ycpicqqrnc lrceaihkdm rcadyqadlk
      661 rraendaaar aslnflegqg disggcrcgv ggvrchprcn ych
//
LOCUS       XP_077502133             234 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113088 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502133
VERSION     XP_077502133.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646007.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..234
                     /product="uncharacterized protein LOC144113088 isoform X1"
                     /calculated_mol_wt=26086
     CDS             1..234
                     /gene="LOC144113088"
                     /coded_by="XM_077646007.1:76..780"
                     /db_xref="GeneID:144113088"
ORIGIN      
        1 mtpcfarcge elpsrrlllw rrqllpdari fivrfpahfs pqldksgskh entsskehcl
       61 yvfwrtadlk krpkrcnrtc qqawlkdcfp evehempflm aseakqssps twavlsagal
      121 atlavlgtyl tvawhpaflp llilplfglc hvlvypkaak rgfigppvir yampeefrct
      181 vlvakpqpdy qtipvekvqp pppaapvrpa thcaaycapi vpafhapppv iavm
//
LOCUS       XP_077502134             187 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113088 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502134
VERSION     XP_077502134.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646008.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="uncharacterized protein LOC144113088 isoform X2"
                     /calculated_mol_wt=19976
     CDS             1..187
                     /gene="LOC144113088"
                     /coded_by="XM_077646008.1:16..579"
                     /db_xref="GeneID:144113088"
ORIGIN      
        1 mcsrrvyspa ggrprdrphq gltlgsrthv pparrppllp vteaaafemp flmaseakqs
       61 spstwavlsa galatlavlg tyltvawhpa flpllilplf glchvlvypk aakrgfigpp
      121 viryampeef rctvlvakpq pdyqtipvek vqppppaapv rpathcaayc apivpafhap
      181 ppviavm
//
LOCUS       XP_077502136             139 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113088 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502136
VERSION     XP_077502136.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646010.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..139
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..139
                     /product="uncharacterized protein LOC144113088 isoform X3"
                     /calculated_mol_wt=14731
     CDS             1..139
                     /gene="LOC144113088"
                     /coded_by="XM_077646010.1:111..530"
                     /db_xref="GeneID:144113088"
ORIGIN      
        1 mpflmaseak qsspstwavl sagalatlav lgtyltvawh paflpllilp lfglchvlvy
       61 pkaakrgfig ppviryampe efrctvlvak pqpdyqtipv ekvqppppaa pvrpathcaa
      121 ycapivpafh apppviavm
//
LOCUS       XP_077502137             152 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113089 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502137
VERSION     XP_077502137.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646011.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..152
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..152
                     /product="uncharacterized protein LOC144113089 isoform X1"
                     /calculated_mol_wt=16534
     CDS             1..152
                     /gene="LOC144113089"
                     /coded_by="XM_077646011.1:63..521"
                     /db_xref="GeneID:144113089"
ORIGIN      
        1 mdasyveccy fmlgganpgl prafmyafnm fwftlvavsa lmgslvhkgy yfisigcvml
       61 ivitcasysi yrtcmgrrlr pvapasdadg ndslfgpppp yeavmirqpd gppdyteaic
      121 intapkeslp gnveserlls depqptavev ek
//
LOCUS       XP_077502138             148 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113089 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502138
VERSION     XP_077502138.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646012.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="uncharacterized protein LOC144113089 isoform X2"
                     /calculated_mol_wt=16086
     CDS             1..148
                     /gene="LOC144113089"
                     /coded_by="XM_077646012.1:148..594"
                     /db_xref="GeneID:144113089"
ORIGIN      
        1 mseccyfmlg ganpglpraf myafnmfwft lvavsalmgs lvhkgyyfis igcvmlivit
       61 casysiyrtc mgrrlrpvap asdadgndsl fgppppyeav mirqpdgppd yteaicinta
      121 pkeslpgnve serllsdepq ptavevek
//
LOCUS       XP_077502139            2945 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: kalirin-like [Amblyomma americanum].
ACCESSION   XP_077502139
VERSION     XP_077502139.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646013.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (GKSP01011825.1) annotated
            using gene prediction method: Gnomon, supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts  :: corrected 2 indels
            assembly gap :: added 1300 transcript bases to patch genome
                            assembly gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..2945
                     /product="LOW QUALITY PROTEIN: kalirin-like"
                     /calculated_mol_wt=330760
     Region          36..176
                     /region_name="SEC14"
                     /note="Domain in homologues of a S. cerevisiae
                     phosphatidylinositol transfer protein (Sec14p);
                     smart00516"
                     /db_xref="CDD:214706"
     Region          189..409
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            308..313
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          553..770
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Region          666..885
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            769..774
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          886..1116
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            991..996
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Site            1116..1121
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1122..1222
                     /region_name="SPEC"
                     /note="Spectrin repeats; smart00150"
                     /db_xref="CDD:197544"
     Region          1270..1441
                     /region_name="RhoGEF"
                     /note="Guanine nucleotide exchange factor for
                     Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous
                     (DH) domain. It appears that PH domains invariably occur
                     C-terminal to RhoGEF/DH domains; cd00160"
                     /db_xref="CDD:238091"
     Site            order(1274,1278,1373,1394..1395,1398..1399,1401..1402,
                     1405..1406,1409..1410,1413,1437,1441)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:238091"
     Region          1448..1574
                     /region_name="PH1_Kalirin_Trio_like"
                     /note="Triple functional domain pleckstrin homology
                     pleckstrin homology (PH) domain, repeat 1; cd13240"
                     /db_xref="CDD:270060"
     Site            order(1465,1505,1507)
                     /site_type="other"
                     /note="Rac1 binding site [polypeptide binding]"
                     /db_xref="CDD:270060"
     Region          <1693..1892
                     /region_name="SH3-RhoG_link"
                     /note="SH3-RhoGEF linking unstructured region; pfam16609"
                     /db_xref="CDD:465196"
     Region          1890..2068
                     /region_name="RhoGEF"
                     /note="Guanine nucleotide exchange factor for
                     Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous
                     (DH) domain. It appears that PH domains invariably occur
                     C-terminal to RhoGEF/DH domains; cd00160"
                     /db_xref="CDD:238091"
     Site            order(1896,1900,1996,2017..2018,2021..2022,2024..2025,
                     2028..2029,2032..2033,2036,2064,2068)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:238091"
     Region          2073..2211
                     /region_name="PH2_Kalirin_Trio_p63RhoGEF"
                     /note="p63RhoGEF pleckstrin homology (PH) domain, repeat
                     2; cd13241"
                     /db_xref="CDD:270061"
     Site            order(2082,2115..2116,2142,2144,2198,2201..2203,
                     2205..2206,2208..2210)
                     /site_type="other"
                     /note="Galpha i/q binding site [polypeptide binding]"
                     /db_xref="CDD:270061"
     Region          2553..2639
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(2628..2629,2631..2632)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          2668..2911
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(2668..2670,2673,2675,2688,2690,2718,2734..2737,2781,
                     2785..2786,2788,2799..2800)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..2945
                     /gene="LOC144113091"
                     /coded_by="XM_077646013.1:118..8955"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 1 base in 1 codon;
                     deleted 1 base in 1 codon; added 1300 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144113091"
ORIGIN      
        1 mcatksstdg padsdgafry ksgpsrmmeg ahellpllqe rlmilsggrd lrggpvltfp
       61 arsrpdrlkp eemrkvltyl ssipceeare lgfsvvidmr gatwatvkpi lkvlqeyfpa
      121 kihaayiikp enfwqkqrts mgsskykfet nlispealsk yidpsqltad ldgtlpydhg
      181 qwlelrmale kflwktseml erlemmkedl vstkfaedvt gakrmiednl nakkrvlqip
      241 vdevdlegqq vlhrlgvssg gsgssdsgys gggdpagpqv grlleslrla rqhvlqlwhv
      301 rkvqldqalq lslyqqdahk mlewiyhnke rflmnyveig hsiqdakvlq eehdhftmas
      361 mnvyvninri lvtgtrlieg ghyaagtvqq ivaaldhawk efaagldert tvlslsvlfh
      421 qkaqqylanv pswsksseis siprevadle elihkhqsly eamcqaytev hsaskkllyq
      481 ldhlvqvchp sksethkhga aegknlllgc qagdysegak hvlsviheil gqhraleskw
      541 htkklklhqr lalrlfqedv rqvldwlekh gevflrknpg igrnlvrarv lqkshehfen
      601 vaqntytnae kllaaaeela qtgecnadei cgvaqdledq itsfatrveq rrqllqlavi
      661 ffthdkelss wfeelraelh snkvadtvea aeqlleqftq qrdstidaav stisegetll
      721 eelrslgmna etdatgsyva vegtlealtr trhelealws nrklqldlcl qlrlferdsi
      781 elssqfelwm kelnqmelsr elsqaernlq lhtdsvahmq qavfqllqrg qelsqvless
      841 gvqlmadsqy daqnriqtll eflheremdi edlaevkrvr leqciqlcql ekdasqvntw
      901 irngeamlsa tfaiptclpe aeqsrsqheq fqlaiektha saiqiqqrae slvqanhydp
      961 aavravaeav dtwwhrmmth aedrhrmvta alrfyktaeq vysvldsler eyrrdedwca
     1021 ageelegtdr gvqlaqlltk hqekkeaflk actmarrnae tflkytarcs qhcaghgdan
     1081 crgpeakvka lmeqllkqen kvleywtvrk krldqcqqyv lfersakqal gwiketgehy
     1141 ltshnglges reeterllke hnefkgnake trekvrlllq ladslvergh ahasaikcwv
     1201 aavdkgykdf slrmdqyrsq leqklgiqve etkelsldrn sdpnleskvk esavkelnee
     1261 krksarrkef imaellqter tyvkdletci htymaelrna evnrppgivg kehvlfgnme
     1321 eiyefhnsif lkelekyetm pedvghcfvt waqkfeiyvk ycknkpesna lliqhagtff
     1381 eevqhkhgvp hpipaylikp vqritkyqll xkdllacced gvqgeirdgl evmlnvpkka
     1441 ndamhlslld gcdvsldqlg evvlqdsfqv fdsralirkg rerhiflfel yllfskemkd
     1501 sngkvkyvyk qklmtsevgi tehiegdeck favwtavwte hgptsenkii lkassletkq
     1561 twvkklrqvi qetcfnstls tlnlnkstkl tsklfsnrss rdfedssqde gltdlqervs
     1621 vasfgssntt dsekggtdpd dqtpsssqge paaftewhvp ardggrrqel lpadlspggg
     1681 gppvpplpsp gnkrrgtfrk wltnpvrkls hgrldkadpa sakgnptpps kklappaavs
     1741 qssklvsyke dmgnkssspg ppskpseite vnkaeqsaqq kccvstksss vdadpggtae
     1801 eeevcelppp meiqdhlfkp qqtpdftidp aepeeralgg eledtskekq alitqdddse
     1861 tttsqedphe gslettvpdg rerekslqkr sfvlkelvdt eqqyvtdlgy ivegymavmq
     1921 kgevpmpedl kngkdkiifg nieaiyewhr dlflaelekc leeperlgll frryerrlnm
     1981 yvvycqnkpk seyivseyid tyfeeirqkl ghklqlpdll ikpvqrimky qlllkdilky
     2041 teraqlhkea edlrkavhim hvvpkaandm mnvgrlqgfd gkitaqgkll lqglllvsep
     2101 stgakfrerq vflfeqiiil seavgaktpf snqayiyknh lqvnkmslne hsedgdplkf
     2161 tlcskdphqe gvsfviqtps mearnkwvsn irailetqld flraiqspia yqkeltkdis
     2221 apelsslwnp slrktlshpa aahkctrgsv atadsgtsks mrqrdkklvr paeaedrkap
     2281 qhspgkgkrt flegfrntlr pkaaakpeaa lcfshsldsa sraattlepa adgrrwsete
     2341 seetspspgq eqrgrspnde rpspwrrvas ftssgnrqed gaslcngrpw slrlkktyka
     2401 dkkqrksqdd tslsaegnsf telmpsavaa apgscavlrc qmtppgsagg avswrlpdgg
     2461 svrasgplhs apvlfrmgas vegelpaire hprsladrcv arlhadgsss levadcgpqd
     2521 aglyvcshva dgatqatatr rllvtgnypn esgrpqllka egtklqlkwe paqgaddcpm
     2581 tgyvveksik glhfwepagp pttfctqvih cepgtyafrv vsyselgtsn pgppsdtitv
     2641 addaaksqsg vvewkkdfsg fvevaecgrg rfsvvkkcin natgereaak iigfqqqsra
     2701 tvehecqila slsyeaivel lecyrtpssh ilvfpfiegl rlfdhicvqw sfysehtash
     2761 ylaqllgald ylhlcgiahl dikpenllve ecsdrlklid fgdarkvlga eckmsvigta
     2821 efmapevlrc rplstrtdlw stgvllyvfl vglspflgat eletcaniea aryaiptsas
     2881 iseeasdlls kllaidtqkr lsaskalahh wikeapsaqt lsssplrdfi krrdemvlma
     2941 eltss
//
LOCUS       XP_077502140             252 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113093 [Amblyomma americanum].
ACCESSION   XP_077502140
VERSION     XP_077502140.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646014.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..252
                     /product="uncharacterized protein LOC144113093"
                     /calculated_mol_wt=29599
     Region          7..81
                     /region_name="RRM_RBM7_like"
                     /note="RNA recognition motif (RRM) found in RNA-binding
                     protein 7 (RBM7) and similar proteins; cd12336"
                     /db_xref="CDD:409773"
     Region          <10..221
                     /region_name="PABP-1234"
                     /note="polyadenylate binding protein, human types 1, 2, 3,
                     4 family; TIGR01628"
                     /db_xref="CDD:130689"
     CDS             1..252
                     /gene="LOC144113093"
                     /coded_by="XM_077646014.1:134..892"
                     /db_xref="GeneID:144113093"
ORIGIN      
        1 mdnelrdrtl wcgnlhyrvt eevlrelfiq agpvedvkip kdaagrarnf afitfhhpes
       61 igytlalmdg itlygrtikm errpqaavdn tyvdlmanyc ayykgltfgp aqrppnmynr
      121 gdaprqsfas qgqglqgthl pqgmpihyhh qqqqchqrgg vppgpeysrn ysrdydrypp
      181 drendrrdnd hydrrarape pyarydrefr venghpdmrs thrgsgrydr ryseeqqrsr
      241 sdhrrdhhgr rh
//
LOCUS       XP_077502141             193 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113095 [Amblyomma americanum].
ACCESSION   XP_077502141
VERSION     XP_077502141.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646015.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..193
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..193
                     /product="uncharacterized protein LOC144113095"
                     /calculated_mol_wt=20426
     CDS             1..193
                     /gene="LOC144113095"
                     /coded_by="XM_077646015.1:16..597"
                     /db_xref="GeneID:144113095"
ORIGIN      
        1 msellfrnlg liapavvlvw prelitwrsa qkdpvcgltg kpcpsalgsa crgrrlsrts
       61 vdrkvrtara fcqqhrrtar ssslpapalt rmdsteadsg edvqlerprn avllgcsaas
      121 ivatavgvfa giflhpaivl llvlgnaasv alalrcgyra vqpeplgnaa kapdgkpvlp
      181 viqiyppkmv tel
//
LOCUS       XP_077502143             159 aa            linear   INV 25-NOV-2025
DEFINITION  protein FAM136A-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502143
VERSION     XP_077502143.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646017.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..159
                     /product="protein FAM136A-like isoform X1"
                     /calculated_mol_wt=17855
     Region          5..150
                     /region_name="DUF842"
                     /note="Eukaryotic protein of unknown function (DUF842);
                     pfam05811"
                     /db_xref="CDD:461747"
     CDS             1..159
                     /gene="LOC144113101"
                     /coded_by="XM_077646017.1:257..736"
                     /db_xref="GeneID:144113101"
ORIGIN      
        1 maeeaarrvq taidnmvnel egthlrrila pltviggrrg nfsphyinkg nvhrcavkcc
       61 ddsslsrert racimncsep iekaqskveg elgnfqervr mcvvqcendv rdqmdpqtte
      121 aeavkfknqy dacvikcadk higllpqllq rlkdslkhc
//
LOCUS       XP_077502144             139 aa            linear   INV 25-NOV-2025
DEFINITION  protein FAM136A-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502144
VERSION     XP_077502144.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646018.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..139
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..139
                     /product="protein FAM136A-like isoform X2"
                     /calculated_mol_wt=15675
     Region          5..130
                     /region_name="DUF842"
                     /note="Eukaryotic protein of unknown function (DUF842);
                     pfam05811"
                     /db_xref="CDD:461747"
     CDS             1..139
                     /gene="LOC144113101"
                     /coded_by="XM_077646018.1:230..649"
                     /db_xref="GeneID:144113101"
ORIGIN      
        1 maeeaarrvq taidnmvnel egthlrrilg nvhrcavkcc ddsslsrert racimncsep
       61 iekaqskveg elgnfqervr mcvvqcendv rdqmdpqtte aeavkfknqy dacvikcadk
      121 higllpqllq rlkdslkhc
//
LOCUS       XP_077502145             515 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113107 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502145
VERSION     XP_077502145.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646019.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..515
                     /product="uncharacterized protein LOC144113107 isoform X1"
                     /calculated_mol_wt=56825
     CDS             1..515
                     /gene="LOC144113107"
                     /coded_by="XM_077646019.1:156..1703"
                     /db_xref="GeneID:144113107"
ORIGIN      
        1 mtdvmslqsl fvpgasrsst iklncdadst tdssgrlmlp eivagtpaia mlpqrkgsfl
       61 rgtfygkngg ewadeetgee tsdfirtlqk qlkemtisga vyrlmqnhea hgflnaerrf
      121 pcantltgvy siyrgasfdv iqshclssvp pfndrkpnlg rlegwlvvtl pwdhaglsfg
      181 aeiqkwcgep ellkapqrcf tsshrhqcvv kkdvttlrsl prflvrqsdd mlmlvlhasa
      241 adpttvsgsf cackaafrmf ftdcrspeev ysvavatpfa kdslldaykv vslqkilfif
      301 lyktksavgs wmtisvgtgf drlqalvlep pmacitergq vklvftqfsk fssvesvern
      361 kkeaegashk skapliarqq rslsescayq ipertrrgil kngidwrrti sessddcfsa
      421 tsldsdlscp asslesiqpk ktvsfsdhvs hivyranssv larrrrnqkk aankkkaaar
      481 ralnrfdstd tsgissgsea sledisrscq pcppe
//
LOCUS       XP_077502146             508 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113107 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502146
VERSION     XP_077502146.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646020.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..508
                     /product="uncharacterized protein LOC144113107 isoform X2"
                     /calculated_mol_wt=56053
     CDS             1..508
                     /gene="LOC144113107"
                     /coded_by="XM_077646020.1:155..1681"
                     /db_xref="GeneID:144113107"
ORIGIN      
        1 mtdvmslqsl fvpgasrsst iklncdadst tdssgrlmlp eivagtpaia mlpqrkgsfl
       61 rgtfygkngg ewadeetgee tsdfirtlqk qlkemtisga vyrlmqnhea hgflnaerrf
      121 pcantltgvy siyrgasfdv iqshclssvp pfndrkpnlg rlegwlvvtl pwdhaglsfg
      181 aeiqkwcgep ellkapqrcf tsshrhqcvv kkdvttlrsl prflvrqsdd mlmlvlhasa
      241 adpttvsgsf cackaafrmf ftdcrspeev ysvavatpfa kdslldaykv vslqkilfif
      301 lyktksavgs wmtisvgtgf drlqalvlep pmacitertq fskfssvesv ernkkeaega
      361 shkskaplia rqqrslsesc ayqipertrr gilkngidwr rtisessddc fsatsldsdl
      421 scpasslesi qpkktvsfsd hvshivyran ssvlarrrrn qkkaankkka aarralnrfd
      481 stdtsgissg seasledisr scqpcppe
//
LOCUS       XP_077502147             583 aa            linear   INV 25-NOV-2025
DEFINITION  frizzled 2 [Amblyomma americanum].
ACCESSION   XP_077502147
VERSION     XP_077502147.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646021.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..583
                     /product="frizzled 2"
                     /calculated_mol_wt=65099
     Region          22..141
                     /region_name="CRD_FZ5_like"
                     /note="Cysteine-rich Wnt-binding domain (CRD) of receptors
                     similar to frizzled 5; cd07456"
                     /db_xref="CDD:143565"
     Site            order(30,32..34,36..38)
                     /site_type="other"
                     /note="putative Wnt binding site [polypeptide binding]"
                     /db_xref="CDD:143565"
     Region          222..527
                     /region_name="7tmF_FZD5_FZD8-like"
                     /note="class F frizzled subfamilies 5, 8 and related
                     proteins; member of 7-transmembrane G protein-coupled
                     receptors; cd15035"
                     /db_xref="CDD:320163"
     Site            order(222,231,281,296,379,384,386,389..390,395,471,
                     474..475,491,496,499..500)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:320163"
     Region          230..255
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320163"
     Region          264..285
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320163"
     Region          311..337
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320163"
     Region          354..370
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320163"
     Region          392..421
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320163"
     Region          442..469
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320163"
     Region          489..514
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320163"
     CDS             1..583
                     /gene="fz2"
                     /coded_by="XM_077646021.1:322..2073"
                     /db_xref="GeneID:144113109"
ORIGIN      
        1 mrphvlplll lasagggwsr erceditipm ckgigynqts mpnqfnqdtq deagmevhqf
       61 wplveiqcsd dlkfflcsly tpicmeeyag svpacrsvce raragcapim rqygfawper
      121 mncdalpqyg dpdqlcmdsk egarpdsadt slvaappapp pskprggsrp rqrerqpgrg
      181 tasgdcdcpc qaplintsdp hfyarglssr ppecalpcrm vyfngydqtf atywvstwav
      241 lccvstsltv ltflidttrf ryperpiifl sfcylmvsag ylvrlamghe piacdgnvvr
      301 yyvssrpfac tlvfillyff gmassmwwvi ltltwllaag lkwgneaiag ysqyfhmvaw
      361 avptvktiva vllsavdgds vagvcyvgnq dltnlrafvl gplcvyllvg tsfllagfvs
      421 lfrirsvira qarakadkle klmirigifs vlytvpatvv iacyfyehhy rdawlrrlrc
      481 pcprnneapr phffmfmvky fmclvvgits gfwiwsgktl dswwnfyarl cggrrrrgrs
      541 vagggvgdfp dphvrtfkqs mspvshigql sshggkqlpl shv
//
LOCUS       XP_077502148             387 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113110 [Amblyomma americanum].
ACCESSION   XP_077502148
VERSION     XP_077502148.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646022.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..387
                     /product="uncharacterized protein LOC144113110"
                     /calculated_mol_wt=42910
     Region          70..165
                     /region_name="BACK"
                     /note="BTB And C-terminal Kelch; smart00875"
                     /db_xref="CDD:197943"
     Region          211..381
                     /region_name="TLD"
                     /note="cl02144"
                     /db_xref="CDD:445683"
     CDS             1..387
                     /gene="LOC144113110"
                     /coded_by="XM_077646022.1:426..1589"
                     /db_xref="GeneID:144113110"
ORIGIN      
        1 mgkihlqdst vfealalahe lgieelrqsc edhvtstmsi hnacmflasa lamedrvsss
       61 gtkggksfvd rctayvgena mecvktsaff nlpkdalihl vssdylamee edvwravlsw
      121 akhhagvtqp tahwteeera ricqqllgvi nhvrlllids qvfaeevept gavpmeltle
      181 ryryaalpsk fthleerhlq prtslrlfqg sqlltgpklr lqqvlnawyg vpkqlwrlly
      241 rasnhgysae sfhhlcdghs ptfvialgsq gqlcggfsdv pwgktggrgr yipsekaflf
      301 tlinnadlpp tkfdivkkmf aishhpeygp ifgagadlsi anhcnanmes ysnlphsydg
      361 egasctllmg dynfsvldye vfttvgk
//
LOCUS       XP_077502149             357 aa            linear   INV 25-NOV-2025
DEFINITION  dnaJ heat shock protein family member shriveled [Amblyomma
            americanum].
ACCESSION   XP_077502149
VERSION     XP_077502149.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646023.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..357
                     /product="dnaJ heat shock protein family member shriveled"
                     /calculated_mol_wt=40169
     Region          26..345
                     /region_name="DnaJ_bact"
                     /note="chaperone protein DnaJ; TIGR02349"
                     /db_xref="CDD:274090"
     CDS             1..357
                     /gene="shv"
                     /coded_by="XM_077646023.1:71..1144"
                     /db_xref="GeneID:144113111"
ORIGIN      
        1 maspalglls fsvlllliaq slagrdfysi lgvsrsanvn qikkayrkla kelhpdknkd
       61 dpraqekfqd lgaayevlsd pekrktydrh geeglkhdaf ggsdpfasff gdfgffgegs
      121 rneqreiprg advvmdlwvt leelyagnfv evvrnkpvvk pakgtrrcnc rqemvtrqlg
      181 pgrfqmmqqt vcdecpnvkl vneeklleve veagmkdgqe qvfvaegeph idgepgdlkl
      241 rirtmphhaf errgddlytn vtisltdalv gfeteithld ghkvlvtrek itwpgarlrk
      301 knegmpnyen nnqkgtlyvt fdvdfpkgdl saedkeaikk ilkeesktki ynglrgy
//
LOCUS       XP_077502150             281 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113113 [Amblyomma americanum].
ACCESSION   XP_077502150
VERSION     XP_077502150.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646024.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..281
                     /product="uncharacterized protein LOC144113113"
                     /calculated_mol_wt=32135
     Region          13..100
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..281
                     /gene="LOC144113113"
                     /coded_by="XM_077646024.1:69..914"
                     /db_xref="GeneID:144113113"
ORIGIN      
        1 maaavgprav straswtdre vecflalise kkvsealdsr rqrnqdvfkd lqaemanlgy
       61 hwtwqqlrnc wknlkkrfta erleqersga apsawkwydh msallshrpm vqareygvds
      121 qdvppdygdn sqldisdadt rteeddadil qeqsepstss rsppqkrrrm geaklekvll
      181 nvqrkneefw tkqseiqfqq qkqlladenk hmqellqgvs ttflqgtqtl mgqffsqqaa
      241 lvaslqhmps fpqfmgppnm nlqhqfpppp patntpptpk h
//
LOCUS       XP_077502151             655 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113116 [Amblyomma americanum].
ACCESSION   XP_077502151
VERSION     XP_077502151.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646025.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..655
                     /product="uncharacterized protein LOC144113116"
                     /calculated_mol_wt=69397
     Region          <57..315
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          <459..>513
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     CDS             1..655
                     /gene="LOC144113116"
                     /coded_by="XM_077646025.1:216..2183"
                     /db_xref="GeneID:144113116"
ORIGIN      
        1 madgedalhl ldedgirsal lrhrrsssvg csrslrklia easitlqrqq eavrivtaaq
       61 gtppsieqea sslpvhdatp ledgqpaanv ellsetpplg spilkdvlpt psppalrryd
      121 satprvekpt aaapstaatg tqtspsnasd dhgpdeeegd vaatthqepq taeavqsgns
      181 cavttnleea fseadylras laytkrqgnp phrrapspnt attkrgspsk kkkrspperr
      241 apspdaaann vsvgepsgra pvttgniaps aanakpqran qrrrfqaaqp digpnskrfs
      301 gpppqqgngn fgsptilfhp tgrmanflst tkdaiaaqis klpgvkavrv nlrrnvvaad
      361 ahptadlqpl ldvsiicgvk vrakeaggct ctgrvfrvdr alstddimag iesrvpvisc
      421 trsgsdiilr fagqeppqei slfklrrsvr arlprplqcf qcgtyghata actakprclr
      481 cgnnhhasqc dsnkprcvnc rgthgatepr cprwqeerqi aialaapnnr ltrqqiardi
      541 qkerankpsq dtapnsvdaa hnsglspeat appavsaaaq nytqtppqta rsfrdvvvgr
      601 kpdgttvnqp dqrdvviaal aaavraliae lpstspahql caaalaaqqa lskhd
//
LOCUS       XP_077502152             267 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113118 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502152
VERSION     XP_077502152.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646026.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..267
                     /product="uncharacterized protein LOC144113118 isoform X1"
                     /calculated_mol_wt=29749
     Region          <57..>98
                     /region_name="Med15"
                     /note="ARC105 or Med15 subunit of Mediator complex
                     non-fungal; pfam09606"
                     /db_xref="CDD:312941"
     Region          110..183
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..267
                     /gene="LOC144113118"
                     /coded_by="XM_077646026.1:168..971"
                     /db_xref="GeneID:144113118"
ORIGIN      
        1 mavadvsaam lvlidgvetr vyavldehgt hmknddglly etegglrisv lrveepmrnt
       61 ppptvpaave dssrgapspl wqttpppvwq ptpspgpscy spppsggger dawherksrf
      121 litkykeakd nigrkggfrt kkamwekltd qinsefgchl tplqvenkwk tmeraykksk
      181 akkwlvwtfa ssllvrtvwt adisvvcsss ypqkmifdvf ffyrelsevl erehhitpva
      241 llapgrivan gekpsyptan fsatpfa
//
LOCUS       XP_077502153             234 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113118 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502153
VERSION     XP_077502153.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646027.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..234
                     /product="uncharacterized protein LOC144113118 isoform X2"
                     /calculated_mol_wt=25914
     Region          60..>103
                     /region_name="DUF3432"
                     /note="Domain of unknown function (DUF3432); pfam11914"
                     /db_xref="CDD:403204"
     Region          110..183
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..234
                     /gene="LOC144113118"
                     /coded_by="XM_077646027.1:168..872"
                     /db_xref="GeneID:144113118"
ORIGIN      
        1 mavadvsaam lvlidgvetr vyavldehgt hmknddglly etegglrisv lrveepmrnt
       61 ppptvpaave dssrgapspl wqttpppvwq ptpspgpscy spppsggger dawherksrf
      121 litkykeakd nigrkggfrt kkamwekltd qinsefgchl tplqvenkwk tmeraykksk
      181 akkwlvwtfa ssllvrtgtl rgarkraphy scgiactrkn scvslqlnts fflg
//
LOCUS       XP_077502154             182 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113118 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502154
VERSION     XP_077502154.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646028.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..182
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..182
                     /product="uncharacterized protein LOC144113118 isoform X3"
                     /calculated_mol_wt=19632
     Region          <57..>98
                     /region_name="Med15"
                     /note="ARC105 or Med15 subunit of Mediator complex
                     non-fungal; pfam09606"
                     /db_xref="CDD:312941"
     CDS             1..182
                     /gene="LOC144113118"
                     /coded_by="XM_077646028.1:168..716"
                     /db_xref="GeneID:144113118"
ORIGIN      
        1 mavadvsaam lvlidgvetr vyavldehgt hmknddglly etegglrisv lrveepmrnt
       61 ppptvpaave dssrgapspl wqttpppvwq ptpspgpscy spppsggger dawherksrf
      121 litkykeakd nigrkggfre lsevlerehh itpvallapg rivangekps yptanfsatp
      181 fa
//
LOCUS       XP_077502155             921 aa            linear   INV 25-NOV-2025
DEFINITION  structural maintenance of chromosomes protein 5-like, partial
            [Amblyomma americanum].
ACCESSION   XP_077502155
VERSION     XP_077502155.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646029.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (DAPXPI010019966.1) annotated
            using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 761 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: incomplete on the carboxy end.
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..>921
                     /product="structural maintenance of chromosomes protein
                     5-like"
     Region          7..>206
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            65..72
                     /site_type="other"
                     /note="Walker A/P-loop"
                     /db_xref="CDD:213179"
     Site            188..191
                     /site_type="other"
                     /note="Q-loop/lid"
                     /db_xref="CDD:213179"
     Region          <235..>920
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..921
                     /gene="LOC144113121"
                     /coded_by="XM_077646029.1:224..>2986"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 761 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144113121"
ORIGIN      
        1 mekyangavv riqledfmsg gqnrlltawc rrfdvcqgsc nwekkgrayr qmevrpgpnl
       61 nvvvgvngtg ksslvcalcl glcgtpfqla raaspadyir tganrariei elcnhergny
      121 viertilpnk svwkvntvlv aqkkfgrkss ailacgsasq cfsaekmtkq veelvaslni
      181 qvgnlcqflp qdrvaafvcm sqqelleste navgsvdlvh mhqrlielqq icgsqeeslr
      241 aqqlqlsqer qkleqlspdm hslesykeve lridrlrqkl awlvyeearc allekkqwlq
      301 eiknevrnqd qevaqlkgwv daasrryaql saadkqlgea vaahmqslea tskkvfelee
      361 qfssakrelr rrlqeeearv srmkalaesi adieqemadl ardkseeerv rvdlqqcnqa
      421 inriqrerae adifigcrqr estvlvqqmm rlqdvsnqrm ellrrrsrea yeatlwlrqn
      481 egrfkgkvya pimtqidlle psdakyveaq iptndlvafv aerqddlnaf ltamrdtcnl
      541 svngvvmpse sldafqprrp lsqisqygfr ayvqslftap dgvmrylckr yrvhdipvgs
      601 aatedclsql rtlginrfft eenlytvrvs eydpsrfstm sseleppsll imsvdtdtle
      661 eleqrkqvls deiaakvaeg rkldseercl qqqqeelrra kkrllenagr lrqlsiiled
      721 kqatlqtmes aaidldevrr ktlcqqqetc acrmvqvktl tetvklcget sraraehrlh
      781 mmtaaadqek aegmlrvaeq qrfslqvale dlqkrlaayq revlvkrhka qeatkcsstt
      841 kqippelvke fkrlprtake vnnqllleeq alscmlpvda svereywqck aavsrleqev
      901 agseqqlsla raemaetgar w
//
LOCUS       XP_077502156             162 aa            linear   INV 25-NOV-2025
DEFINITION  structural maintenance of chromosomes protein 5-like [Amblyomma
            americanum].
ACCESSION   XP_077502156
VERSION     XP_077502156.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646030.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..162
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..162
                     /product="structural maintenance of chromosomes protein
                     5-like"
                     /calculated_mol_wt=17563
     Region          <38..138
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            58..67
                     /site_type="other"
                     /note="ABC transporter signature motif"
                     /db_xref="CDD:213179"
     Site            82..87
                     /site_type="other"
                     /note="Walker B"
                     /db_xref="CDD:213179"
     Site            90..93
                     /site_type="other"
                     /note="D-loop"
                     /db_xref="CDD:213179"
     Site            116..122
                     /site_type="other"
                     /note="H-loop/switch region"
                     /db_xref="CDD:213179"
     CDS             1..162
                     /gene="LOC144113123"
                     /coded_by="XM_077646030.1:83..571"
                     /db_xref="GeneID:144113123"
ORIGIN      
        1 mniatyelve agpkeattha gvslnhspdp sqydkygvti svkfhdetpl aelsaiyhsg
       61 gersvatvly imalqeqttv pfrvvdeinq gmdsdnerkv femmmntaak nsaqyllvtp
      121 kllpdlpyte dvtmlflmll dspitegidv lagapvgllg dt
//
LOCUS       XP_077502157             113 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113124 [Amblyomma americanum].
ACCESSION   XP_077502157
VERSION     XP_077502157.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646031.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..113
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..113
                     /product="uncharacterized protein LOC144113124"
                     /calculated_mol_wt=12274
     Region          <4..>82
                     /region_name="Extradiol_Dioxygenase_3B_like"
                     /note="Subunit B of Class III Extradiol ring-cleavage
                     dioxygenases; cl00599"
                     /db_xref="CDD:444999"
     CDS             1..113
                     /gene="LOC144113124"
                     /coded_by="XM_077646031.1:144..485"
                     /db_xref="GeneID:144113124"
ORIGIN      
        1 mdlnklcgpw licssdsdep vqpyahsiha pptppnaarl mngsedelam pvvgghcsev
       61 mkakqppvlq frktakrqrt dgsfsskgqr avkanrnepy ssstwiwpls tsp
//
LOCUS       XP_077502158             295 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113125 [Amblyomma americanum].
ACCESSION   XP_077502158
VERSION     XP_077502158.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646032.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..295
                     /product="uncharacterized protein LOC144113125"
                     /calculated_mol_wt=31636
     Region          <139..238
                     /region_name="DUF4371"
                     /note="Domain of unknown function (DUF4371); cl46273"
                     /db_xref="CDD:480613"
     CDS             1..295
                     /gene="LOC144113125"
                     /coded_by="XM_077646032.1:260..1147"
                     /db_xref="GeneID:144113125"
ORIGIN      
        1 msskkyfqaf ipsystdfpc iqksrkgeky afctvcrcdl niahagrrdi llhlgtkkhk
       61 dsaqivdtne klggyfsasv dqgtiraecl ftafliehnv pftaadhagd lfrkmfpnsk
      121 eavaytcart ktaaiakema nqqvssvvsc lqsgpfsvat dgsndtgnda klypivvtfv
      181 dgivhtalla mptlegdstg rnigalvldt lkahnvpvrn cvgmacdnap vmigskngva
      241 avlkenheni ailgccchli nlaaqkgsac lpvsvdevli dvyyylekss kgktn
//
LOCUS       XP_077502159             306 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113126 [Amblyomma americanum].
ACCESSION   XP_077502159
VERSION     XP_077502159.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646033.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..306
                     /product="uncharacterized protein LOC144113126"
                     /calculated_mol_wt=33076
     Region          <139..238
                     /region_name="DUF4371"
                     /note="Domain of unknown function (DUF4371); cl46273"
                     /db_xref="CDD:480613"
     CDS             1..306
                     /gene="LOC144113126"
                     /coded_by="XM_077646033.1:3190..4110"
                     /db_xref="GeneID:144113126"
ORIGIN      
        1 msskkyfqaf ipsystdfpc iqksrkgeky afctvcrcdl niahagrrdi llhlgtkkhk
       61 dsaqivdtne klggyfsasv dqgtiraecl ftafliehnv pftaadhagd lfrkmfpnsk
      121 eavaytcart ktaaiakema nqqvssvvsc lqsgpfsvat dgsndtgnda klypivvtfv
      181 dgivhtalla mptlegdstg rnigalvldt lkahnvpvrn cvgmacdnap vmigskngva
      241 avlkenheni ailgccchli nlaaqkgsac lpvsvdevli dvyyylekss krkdklrafq
      301 clhdve
//
LOCUS       XP_077502160             409 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113127 [Amblyomma americanum].
ACCESSION   XP_077502160
VERSION     XP_077502160.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646034.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..409
                     /product="uncharacterized protein LOC144113127"
                     /calculated_mol_wt=45748
     Region          5..95
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          256..>408
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..409
                     /gene="LOC144113127"
                     /coded_by="XM_077646034.1:44..1273"
                     /db_xref="GeneID:144113127"
ORIGIN      
        1 mpgcccapgc rsnydggpsv rvykfpadet qraawtkais redfvptkht vvcekhfqts
       61 dfvrfatytd entgnvievp lqrvrlkpaa vpsvfpgcpk ylsqppssre apaekkarle
      121 aaslrkaiel svaaqeeedr rnkicsfgel csalpklkts dfwtvvttqa kvlflelsld
      181 qapavrssvv vlddlsvqvf lggsrlhnlg decvpsqmkd lreldkvlqs veklescasg
      241 sekehqllat vlmlldevnk dyelqenhgw nlqvlkfvrs qvelvlknvp ryppdvlvfs
      301 gllytispha yrfirsslki klphpdtirr lcssyhmnpa leqqdpcfln yaktvlqama
      361 ehertvtlmm deihlqtyfe ykagfvigaa anssdpakta yvfmlqsll
//
LOCUS       XP_077502161             641 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113128 [Amblyomma americanum].
ACCESSION   XP_077502161
VERSION     XP_077502161.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646035.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..641
                     /product="uncharacterized protein LOC144113128"
                     /calculated_mol_wt=70586
     CDS             1..641
                     /gene="LOC144113128"
                     /coded_by="XM_077646035.1:468..2393"
                     /db_xref="GeneID:144113128"
ORIGIN      
        1 mpgsarkvhs rhkfgkkrkk slvgnlkrrp avrpdndetp alndrvgeaa qvgltrsaar
       61 eavsgsarir rdtvmlepsd vlqnktkteq klrelastpa terksrclrd gsasdcllde
      121 asftivcfes lnsllklvkc ktcggndlsf vkdereygla vklslqcasc gdassawssp
      181 rvrgdqkinp fvvnvlaara mqstgnqqta lndifsslnv shrglhnktw qdyvkkkltp
      241 aaaraaeevt qecarsvrel yneldlgnpg niavsydgtw mtrghtshig vgtvielftg
      301 lvldyvvlcn fcagcergpk egdpsyqswr aghlcqknse kkagemevea alilferslk
      361 kcglryttml ldgdsrafla vqeadvygyi kvkkedcinh vqkrmgtalr tllskhkgae
      421 nlggkgkltg slitklssyy awalkshngd veatqkavma tyhhitstde asnhsfcpsg
      481 ldswcqqnaa aargesapkh rynipphvak allpvyeqls drkllerchr gktqnsnesl
      541 hsliwalapk erhaslftve aavaeavmrf nagskrtsaa ilqelglntn amsakrmrek
      601 derreqkcar khsiaeniqg vlkkrhldtt gnqkdyipgg y
//
LOCUS       XP_077502162             340 aa            linear   INV 25-NOV-2025
DEFINITION  lysine-specific demethylase 5-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502162
VERSION     XP_077502162.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646036.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..340
                     /product="lysine-specific demethylase 5-like isoform X1"
                     /calculated_mol_wt=38052
     Region          77..177
                     /region_name="JmjC"
                     /note="JmjC domain, hydroxylase; pfam02373"
                     /db_xref="CDD:396791"
     Region          267..>307
                     /region_name="zf-C5HC2"
                     /note="C5HC2 zinc finger; pfam02928"
                     /db_xref="CDD:460750"
     CDS             1..340
                     /gene="LOC144113129"
                     /coded_by="XM_077646036.1:24..1046"
                     /db_xref="GeneID:144113129"
ORIGIN      
        1 mvhlfirvpl iivimscmpr teicqrapwh kiffvtqgar eelqvpvish psllvsknkl
       61 psyslrapra gawlcgetqh gsvlfhcrge pktwygvpgg kaevfedamr caapelfqaq
      121 pdllhqlvti mnpnilqasg vpifrtdqna gefvvtfprs yhagfnqgyn faeavnfapa
      181 dwlpigrvcv shysmlrrfc vfshdelvck maadperldi slaastyqdm lkmvetereq
      241 rrrllewgit daereafell pdderqcdyc kttcflsavt cscnssklvc iphrehlcec
      301 ppsnhclsdv aapiftslpc vragaagsik yrisslegkd
//
LOCUS       XP_077502163             340 aa            linear   INV 25-NOV-2025
DEFINITION  lysine-specific demethylase 5-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502163
VERSION     XP_077502163.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646037.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..340
                     /product="lysine-specific demethylase 5-like isoform X1"
                     /calculated_mol_wt=38052
     Region          77..177
                     /region_name="JmjC"
                     /note="JmjC domain, hydroxylase; pfam02373"
                     /db_xref="CDD:396791"
     Region          267..>307
                     /region_name="zf-C5HC2"
                     /note="C5HC2 zinc finger; pfam02928"
                     /db_xref="CDD:460750"
     CDS             1..340
                     /gene="LOC144113129"
                     /coded_by="XM_077646037.1:24..1046"
                     /db_xref="GeneID:144113129"
ORIGIN      
        1 mvhlfirvpl iivimscmpr teicqrapwh kiffvtqgar eelqvpvish psllvsknkl
       61 psyslrapra gawlcgetqh gsvlfhcrge pktwygvpgg kaevfedamr caapelfqaq
      121 pdllhqlvti mnpnilqasg vpifrtdqna gefvvtfprs yhagfnqgyn faeavnfapa
      181 dwlpigrvcv shysmlrrfc vfshdelvck maadperldi slaastyqdm lkmvetereq
      241 rrrllewgit daereafell pdderqcdyc kttcflsavt cscnssklvc iphrehlcec
      301 ppsnhclsdv aapiftslpc vragaagsik yrisslegkd
//
LOCUS       XP_077502164             340 aa            linear   INV 25-NOV-2025
DEFINITION  lysine-specific demethylase 5-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502164
VERSION     XP_077502164.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646038.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..340
                     /product="lysine-specific demethylase 5-like isoform X1"
                     /calculated_mol_wt=38052
     Region          77..177
                     /region_name="JmjC"
                     /note="JmjC domain, hydroxylase; pfam02373"
                     /db_xref="CDD:396791"
     Region          267..>307
                     /region_name="zf-C5HC2"
                     /note="C5HC2 zinc finger; pfam02928"
                     /db_xref="CDD:460750"
     CDS             1..340
                     /gene="LOC144113129"
                     /coded_by="XM_077646038.1:24..1046"
                     /db_xref="GeneID:144113129"
ORIGIN      
        1 mvhlfirvpl iivimscmpr teicqrapwh kiffvtqgar eelqvpvish psllvsknkl
       61 psyslrapra gawlcgetqh gsvlfhcrge pktwygvpgg kaevfedamr caapelfqaq
      121 pdllhqlvti mnpnilqasg vpifrtdqna gefvvtfprs yhagfnqgyn faeavnfapa
      181 dwlpigrvcv shysmlrrfc vfshdelvck maadperldi slaastyqdm lkmvetereq
      241 rrrllewgit daereafell pdderqcdyc kttcflsavt cscnssklvc iphrehlcec
      301 ppsnhclsdv aapiftslpc vragaagsik yrisslegkd
//
LOCUS       XP_077502165             335 aa            linear   INV 25-NOV-2025
DEFINITION  lysine-specific demethylase 5-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502165
VERSION     XP_077502165.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646039.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..335
                     /product="lysine-specific demethylase 5-like isoform X2"
                     /calculated_mol_wt=37495
     Region          72..172
                     /region_name="JmjC"
                     /note="JmjC domain, hydroxylase; pfam02373"
                     /db_xref="CDD:396791"
     Region          262..>302
                     /region_name="zf-C5HC2"
                     /note="C5HC2 zinc finger; pfam02928"
                     /db_xref="CDD:460750"
     CDS             1..335
                     /gene="LOC144113129"
                     /coded_by="XM_077646039.1:24..1031"
                     /db_xref="GeneID:144113129"
ORIGIN      
        1 mvhlfirvpl iivimscmpr teicqrapwh kiffvtqgar eelqvpvish pslllpsysl
       61 rapragawlc getqhgsvlf hcrgepktwy gvpggkaevf edamrcaape lfqaqpdllh
      121 qlvtimnpni lqasgvpifr tdqnagefvv tfprsyhagf nqgynfaeav nfapadwlpi
      181 grvcvshysm lrrfcvfshd elvckmaadp erldislaas tyqdmlkmve tereqrrrll
      241 ewgitdaere afellpdder qcdyckttcf lsavtcscns sklvciphre hlcecppsnh
      301 clsdvaapif tslpcvraga agsikyriss legkd
//
LOCUS       XP_077502166             333 aa            linear   INV 25-NOV-2025
DEFINITION  lysine-specific demethylase 5-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502166
VERSION     XP_077502166.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646040.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..333
                     /product="lysine-specific demethylase 5-like isoform X3"
                     /calculated_mol_wt=36804
     Region          70..170
                     /region_name="JmjC"
                     /note="JmjC domain, hydroxylase; pfam02373"
                     /db_xref="CDD:396791"
     Region          260..>300
                     /region_name="zf-C5HC2"
                     /note="C5HC2 zinc finger; pfam02928"
                     /db_xref="CDD:460750"
     CDS             1..333
                     /gene="LOC144113129"
                     /coded_by="XM_077646040.1:41..1042"
                     /db_xref="GeneID:144113129"
ORIGIN      
        1 myppnsggde peepersnts dlsldraegg gareelqvpv ishpsllvsk nklpsyslra
       61 pragawlcge tqhgsvlfhc rgepktwygv pggkaevfed amrcaapelf qaqpdllhql
      121 vtimnpnilq asgvpifrtd qnagefvvtf prsyhagfnq gynfaeavnf apadwlpigr
      181 vcvshysmlr rfcvfshdel vckmaadper ldislaasty qdmlkmvete reqrrrllew
      241 gitdaereaf ellpdderqc dyckttcfls avtcscnssk lvciphrehl cecppsnhcl
      301 sdvaapifts lpcvragaag sikyrissle gkd
//
LOCUS       XP_077502167             328 aa            linear   INV 25-NOV-2025
DEFINITION  lysine-specific demethylase 5-like isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077502167
VERSION     XP_077502167.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646041.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..328
                     /product="lysine-specific demethylase 5-like isoform X4"
                     /calculated_mol_wt=36247
     Region          65..165
                     /region_name="JmjC"
                     /note="JmjC domain, hydroxylase; pfam02373"
                     /db_xref="CDD:396791"
     Region          255..>295
                     /region_name="zf-C5HC2"
                     /note="C5HC2 zinc finger; pfam02928"
                     /db_xref="CDD:460750"
     CDS             1..328
                     /gene="LOC144113129"
                     /coded_by="XM_077646041.1:39..1025"
                     /db_xref="GeneID:144113129"
ORIGIN      
        1 myppnsggde peepersnts dlsldraegg gareelqvpv ishpslllps yslrapraga
       61 wlcgetqhgs vlfhcrgepk twygvpggka evfedamrca apelfqaqpd llhqlvtimn
      121 pnilqasgvp ifrtdqnage fvvtfprsyh agfnqgynfa eavnfapadw lpigrvcvsh
      181 ysmlrrfcvf shdelvckma adperldisl aastyqdmlk mvetereqrr rllewgitda
      241 ereafellpd derqcdyckt tcflsavtcs cnssklvcip hrehlcecpp snhclsdvaa
      301 piftslpcvr agaagsikyr isslegkd
//
LOCUS       XP_077502168             316 aa            linear   INV 25-NOV-2025
DEFINITION  lysine-specific demethylase 5-like isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077502168
VERSION     XP_077502168.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646042.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..316
                     /product="lysine-specific demethylase 5-like isoform X5"
                     /calculated_mol_wt=35675
     Region          77..177
                     /region_name="JmjC"
                     /note="JmjC domain, hydroxylase; pfam02373"
                     /db_xref="CDD:396791"
     Region          267..309
                     /region_name="zf-C5HC2"
                     /note="C5HC2 zinc finger; pfam02928"
                     /db_xref="CDD:460750"
     CDS             1..316
                     /gene="LOC144113129"
                     /coded_by="XM_077646042.1:24..974"
                     /db_xref="GeneID:144113129"
ORIGIN      
        1 mvhlfirvpl iivimscmpr teicqrapwh kiffvtqgar eelqvpvish psllvsknkl
       61 psyslrapra gawlcgetqh gsvlfhcrge pktwygvpgg kaevfedamr caapelfqaq
      121 pdllhqlvti mnpnilqasg vpifrtdqna gefvvtfprs yhagfnqgyn faeavnfapa
      181 dwlpigrvcv shysmlrrfc vfshdelvck maadperldi slaastyqdm lkmvetereq
      241 rrrllewgit daereafell pdderqcdyc kttcflsavt cscnssklvc iphrehlcec
      301 ppsnhclris slegkd
//
LOCUS       XP_077502169             316 aa            linear   INV 25-NOV-2025
DEFINITION  lysine-specific demethylase 5-like isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077502169
VERSION     XP_077502169.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646043.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..316
                     /product="lysine-specific demethylase 5-like isoform X5"
                     /calculated_mol_wt=35675
     Region          77..177
                     /region_name="JmjC"
                     /note="JmjC domain, hydroxylase; pfam02373"
                     /db_xref="CDD:396791"
     Region          267..309
                     /region_name="zf-C5HC2"
                     /note="C5HC2 zinc finger; pfam02928"
                     /db_xref="CDD:460750"
     CDS             1..316
                     /gene="LOC144113129"
                     /coded_by="XM_077646043.1:24..974"
                     /db_xref="GeneID:144113129"
ORIGIN      
        1 mvhlfirvpl iivimscmpr teicqrapwh kiffvtqgar eelqvpvish psllvsknkl
       61 psyslrapra gawlcgetqh gsvlfhcrge pktwygvpgg kaevfedamr caapelfqaq
      121 pdllhqlvti mnpnilqasg vpifrtdqna gefvvtfprs yhagfnqgyn faeavnfapa
      181 dwlpigrvcv shysmlrrfc vfshdelvck maadperldi slaastyqdm lkmvetereq
      241 rrrllewgit daereafell pdderqcdyc kttcflsavt cscnssklvc iphrehlcec
      301 ppsnhclris slegkd
//
LOCUS       XP_077502170             316 aa            linear   INV 25-NOV-2025
DEFINITION  lysine-specific demethylase 5-like isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077502170
VERSION     XP_077502170.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646044.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..316
                     /product="lysine-specific demethylase 5-like isoform X5"
                     /calculated_mol_wt=35675
     Region          77..177
                     /region_name="JmjC"
                     /note="JmjC domain, hydroxylase; pfam02373"
                     /db_xref="CDD:396791"
     Region          267..309
                     /region_name="zf-C5HC2"
                     /note="C5HC2 zinc finger; pfam02928"
                     /db_xref="CDD:460750"
     CDS             1..316
                     /gene="LOC144113129"
                     /coded_by="XM_077646044.1:24..974"
                     /db_xref="GeneID:144113129"
ORIGIN      
        1 mvhlfirvpl iivimscmpr teicqrapwh kiffvtqgar eelqvpvish psllvsknkl
       61 psyslrapra gawlcgetqh gsvlfhcrge pktwygvpgg kaevfedamr caapelfqaq
      121 pdllhqlvti mnpnilqasg vpifrtdqna gefvvtfprs yhagfnqgyn faeavnfapa
      181 dwlpigrvcv shysmlrrfc vfshdelvck maadperldi slaastyqdm lkmvetereq
      241 rrrllewgit daereafell pdderqcdyc kttcflsavt cscnssklvc iphrehlcec
      301 ppsnhclris slegkd
//
LOCUS       XP_077502171             311 aa            linear   INV 25-NOV-2025
DEFINITION  lysine-specific demethylase 5-like isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077502171
VERSION     XP_077502171.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646045.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..311
                     /product="lysine-specific demethylase 5-like isoform X6"
                     /calculated_mol_wt=35119
     Region          72..172
                     /region_name="JmjC"
                     /note="JmjC domain, hydroxylase; pfam02373"
                     /db_xref="CDD:396791"
     Region          262..304
                     /region_name="zf-C5HC2"
                     /note="C5HC2 zinc finger; pfam02928"
                     /db_xref="CDD:460750"
     CDS             1..311
                     /gene="LOC144113129"
                     /coded_by="XM_077646045.1:24..959"
                     /db_xref="GeneID:144113129"
ORIGIN      
        1 mvhlfirvpl iivimscmpr teicqrapwh kiffvtqgar eelqvpvish pslllpsysl
       61 rapragawlc getqhgsvlf hcrgepktwy gvpggkaevf edamrcaape lfqaqpdllh
      121 qlvtimnpni lqasgvpifr tdqnagefvv tfprsyhagf nqgynfaeav nfapadwlpi
      181 grvcvshysm lrrfcvfshd elvckmaadp erldislaas tyqdmlkmve tereqrrrll
      241 ewgitdaere afellpdder qcdyckttcf lsavtcscns sklvciphre hlcecppsnh
      301 clrisslegk d
//
LOCUS       XP_077502172             304 aa            linear   INV 25-NOV-2025
DEFINITION  lysine-specific demethylase 5-like isoform X7 [Amblyomma
            americanum].
ACCESSION   XP_077502172
VERSION     XP_077502172.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646046.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..304
                     /product="lysine-specific demethylase 5-like isoform X7"
                     /calculated_mol_wt=33870
     Region          65..165
                     /region_name="JmjC"
                     /note="JmjC domain, hydroxylase; pfam02373"
                     /db_xref="CDD:396791"
     Region          255..>297
                     /region_name="zf-C5HC2"
                     /note="C5HC2 zinc finger; pfam02928"
                     /db_xref="CDD:460750"
     CDS             1..304
                     /gene="LOC144113129"
                     /coded_by="XM_077646046.1:36..950"
                     /db_xref="GeneID:144113129"
ORIGIN      
        1 myppnsggde peepersnts dlsldraegg gareelqvpv ishpslllps yslrapraga
       61 wlcgetqhgs vlfhcrgepk twygvpggka evfedamrca apelfqaqpd llhqlvtimn
      121 pnilqasgvp ifrtdqnage fvvtfprsyh agfnqgynfa eavnfapadw lpigrvcvsh
      181 ysmlrrfcvf shdelvckma adperldisl aastyqdmlk mvetereqrr rllewgitda
      241 ereafellpd derqcdyckt tcflsavtcs cnssklvcip hrehlcecpp snhclrissl
      301 egkd
//
LOCUS       XP_077502174             139 aa            linear   INV 25-NOV-2025
DEFINITION  bolA-like protein 3 [Amblyomma americanum].
ACCESSION   XP_077502174
VERSION     XP_077502174.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646048.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..139
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..139
                     /product="bolA-like protein 3"
                     /calculated_mol_wt=15451
     Region          71..133
                     /region_name="BolA"
                     /note="BolA-like protein; cl00386"
                     /db_xref="CDD:469753"
     CDS             1..139
                     /gene="LOC144113134"
                     /coded_by="XM_077646048.1:16..435"
                     /db_xref="GeneID:144113134"
ORIGIN      
        1 mrlssgdtvq sapcrpndvp cvreavlkmh kfdtftmsrf ltgfvrrlqv frflsneave
       61 tsgekslssi lqkkfpeaad iqvkdisggc gamyevyvqs sifkdlsrvq qhrlvnealk
      121 aeiqdmhgir ittalpgnq
//
LOCUS       XP_077502175             920 aa            linear   INV 25-NOV-2025
DEFINITION  puromycin-sensitive aminopeptidase [Amblyomma americanum].
ACCESSION   XP_077502175
VERSION     XP_077502175.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646049.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..920
                     /product="puromycin-sensitive aminopeptidase"
                     /calculated_mol_wt=103430
     Region          67..505
                     /region_name="M1_APN-Q_like"
                     /note="Peptidase M1 aminopeptidase N catalytic domain
                     family which includes aminopeptidase N (APN),
                     aminopeptidase Q (APQ), tricorn interacting factor F3, and
                     endoplasmic reticulum aminopeptidase 1 (ERAP1); cd09601"
                     /db_xref="CDD:341064"
     Site            order(184,186,322..325,358..359,362,381,385,439,444)
                     /site_type="active"
                     /db_xref="CDD:341064"
     Region          583..897
                     /region_name="ERAP1_C"
                     /note="ERAP1-like C-terminal domain; pfam11838"
                     /db_xref="CDD:463368"
     CDS             1..920
                     /gene="Psa"
                     /coded_by="XM_077646049.1:95..2857"
                     /db_xref="GeneID:144113135"
ORIGIN      
        1 mlaalvprgv gfvlsrfsvp rascsvssra qrpgvclnic gqlctprhia msgespserl
       61 ptnvrpvnyk ielkpdlkkl afdgrtvvtl qvlepsdvmv lnsldldvks aeyipehgke
      121 lkassiatsk eeeritikfd ttlapgtgql kltysgemng klrgfywvkf kgqngeetfg
      181 gvthfeptda rrafpcwdep alkasfdltl ivpkgytals ntnvvsdaec pgdstlrvit
      241 fartpkmsty lvafvlgeyd yiegtsedgv vvriytpkgk qeqgryglev atralpyyke
      301 yfgiayplpk mdliavpdla aaamenwglv threcallvd eqntsaerkq nialvvthei
      361 ahqwfgnlvt mewwthlwln egfasfiefl cvdylfpkyh iwtqfvtdcy aqameldalq
      421 nshpievpvr hpseideifd disyhkgasi irmlhsyigd dkfregmnvy lskykygntt
      481 tedlwhslae vcqvpveaim ntwvkqkgyp visvtsrqdg enrvlmfsqe kfnadgkatk
      541 dgflwmvpis ittskaptti akqflldsas svvvlegvss aewvkvnvgt vgcyrtlyss
      601 emlsqlipav enkilppldr lglqsdlfal vqsghkstvd ilrlmeayve ednytvwnsi
      661 nsclgklnql lshtdlqpll hvygrrllar ifsklgwdpk pdeshlakll rstvidrlar
      721 fkdpevlaea rrrfdahvaw taiiaadirg avyqatasva drklydeflk lyrntdlqee
      781 knrlsaalag vtnpeliqat lefalsdevk sqdavfviiy caltavgrdl twsffknnkd
      841 avrkrycsgf liarlvkcit enfateekal eielffsqny fpgvervvqq slenirlnaa
      901 wlardtedir qyllrtasss
//
LOCUS       XP_077502176             440 aa            linear   INV 25-NOV-2025
DEFINITION  deoxynucleotidyltransferase terminal-interacting protein 1
            [Amblyomma americanum].
ACCESSION   XP_077502176
VERSION     XP_077502176.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646050.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..440
                     /product="deoxynucleotidyltransferase terminal-interacting
                     protein 1"
                     /calculated_mol_wt=48340
     Region          102..167
                     /region_name="DNTTIP1_dimer"
                     /note="DNTTIP1 dimerization domain; pfam18192"
                     /db_xref="CDD:408020"
     CDS             1..440
                     /gene="LOC144113136"
                     /coded_by="XM_077646050.1:134..1456"
                     /db_xref="GeneID:144113136"
ORIGIN      
        1 mastipeiri yspsdkgddt sdsdrsritg dgsdrksaml frgscevfss dnstmvpwln
       61 tfnmrpynls clpstmayrp scrsqsvais raklgcitss aksldvlrqn lqksinreid
      121 eilqrylekf fkpglenvri nngehsiseq hiqavcrqil eeakkmyhgs ytrgnsptsd
      181 cnidsgrnsp sdlklgrfls kqsaagrrrr dnsdsdseas lqmvkkkkgr pplhqggvls
      241 grstpcklka davkregpkw dparlqpgsq fimgakanka lglgatrgrl yikhpevfky
      301 sgdqedkqwl yehnqmpatg gkaymllved irelaetdey rgspglmlde ivgfsvpdsm
      361 lvkmqaamla mrtdlptgsr rrgtrpadlr hddgsaspae eplgaspfgq gfedvspaas
      421 dvsatmepph tapfelglsp
//
LOCUS       XP_077502177             152 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113137 [Amblyomma americanum].
ACCESSION   XP_077502177
VERSION     XP_077502177.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646051.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..152
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..152
                     /product="uncharacterized protein LOC144113137"
                     /calculated_mol_wt=16134
     CDS             1..152
                     /gene="LOC144113137"
                     /coded_by="XM_077646051.1:169..627"
                     /db_xref="GeneID:144113137"
ORIGIN      
        1 mrtdlkidll gtiqmitstc ndvkqatvan cfgkagleva ghecpealdd ndecledafc
       61 dlshfpgvip seltakdfin idsevqavad ladedivagi agaqegdssd dedncvpeem
      121 rpctaaelas afsliwrrrg emegtglshl tv
//
LOCUS       XP_077502178             378 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113141 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502178
VERSION     XP_077502178.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646052.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..378
                     /product="uncharacterized protein LOC144113141 isoform X1"
                     /calculated_mol_wt=40564
     Region          46..130
                     /region_name="NR_DBD_like"
                     /note="DNA-binding domain of nuclear receptors is composed
                     of two C4-type zinc fingers; cl02596"
                     /db_xref="CDD:413390"
     Site            order(48,51,65,68,85,91,101,104)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143512"
     Site            order(58..60,66..67,69,71,74,77,98..99,102,105,116,119)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143512"
     CDS             1..378
                     /gene="LOC144113141"
                     /coded_by="XM_077646052.1:219..1355"
                     /db_xref="GeneID:144113141"
ORIGIN      
        1 mrptyvttmh yllqstachp fgdrkmeigl sepcmpkeww pnrmnqlckv cgepaagyhf
       61 gaftcegcks ffgrtynnls algdcknsgq cvinkknrts ckscrlkkcl mvgmsksgsr
      121 ygrrsnwfki hcliqdqaem nsriaeqhhq lgaaasaaah pltalydkdm lgygakteeg
      181 ngtssplssd kppsspassy pppsssastf astpsafetk ssngesptnt daftyhpmsp
      241 vspisplhps kfffhpgagv ffgkapvthn glsklyadgg gavaypyspr ylplaplkpy
      301 rdflhpstlv vngecladlp eqekpidlsv rkpcspsasp agghaddvda qdcdvkpall
      361 apsssgaeip ldlrsakk
//
LOCUS       XP_077502179             340 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113141 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502179
VERSION     XP_077502179.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646053.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..340
                     /product="uncharacterized protein LOC144113141 isoform X2"
                     /calculated_mol_wt=35976
     Region          8..92
                     /region_name="NR_DBD_like"
                     /note="DNA-binding domain of nuclear receptors is composed
                     of two C4-type zinc fingers; cl02596"
                     /db_xref="CDD:413390"
     Site            order(10,13,27,30,47,53,63,66)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143512"
     Site            order(20..22,28..29,31,33,36,39,60..61,64,67,78,81)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143512"
     CDS             1..340
                     /gene="LOC144113141"
                     /coded_by="XM_077646053.1:182..1204"
                     /db_xref="GeneID:144113141"
ORIGIN      
        1 mtlgmmnqlc kvcgepaagy hfgaftcegc ksffgrtynn lsalgdckns gqcvinkknr
       61 tsckscrlkk clmvgmsksg srygrrsnwf kihcliqdqa emnsriaeqh hqlgaaasaa
      121 ahpltalydk dmlgygakte egngtsspls sdkppsspas sypppsssas tfastpsafe
      181 tkssngespt ntdaftyhpm spvspisplh pskfffhpga gvffgkapvt hnglsklyad
      241 gggavaypys prylplaplk pyrdflhpst lvvngeclad lpeqekpidl svrkpcspsa
      301 spagghaddv daqdcdvkpa llapsssgae ipldlrsakk
//
LOCUS       XP_077502180             353 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113141 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502180
VERSION     XP_077502180.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646054.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..353
                     /product="uncharacterized protein LOC144113141 isoform X3"
                     /calculated_mol_wt=37628
     Region          21..105
                     /region_name="NR_DBD_like"
                     /note="DNA-binding domain of nuclear receptors is composed
                     of two C4-type zinc fingers; cl02596"
                     /db_xref="CDD:413390"
     Site            order(23,26,40,43,60,66,76,79)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143512"
     Site            order(33..35,41..42,44,46,49,52,73..74,77,80,91,94)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143512"
     CDS             1..353
                     /gene="LOC144113141"
                     /coded_by="XM_077646054.1:229..1290"
                     /db_xref="GeneID:144113141"
ORIGIN      
        1 meiglsepcm pkewwpnrmn qlckvcgepa agyhfgaftc egcksffgrt ynnlsalgdc
       61 knsgqcvink knrtsckscr lkkclmvgms ksgsrygrrs nwfkihcliq dqaemnsria
      121 eqhhqlgaaa saaahpltal ydkdmlgyga kteegngtss plssdkppss passypppss
      181 sastfastps afetkssnge sptntdafty hpmspvspis plhpskfffh pgagvffgka
      241 pvthnglskl yadgggavay pysprylpla plkpyrdflh pstlvvngec ladlpeqekp
      301 idlsvrkpcs psaspaggha ddvdaqdcdv kpallapsss gaeipldlrs akk
//
LOCUS       XP_077502181             575 aa            linear   INV 25-NOV-2025
DEFINITION  spermatogenesis-associated serine-rich protein 2-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077502181
VERSION     XP_077502181.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646055.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..575
                     /product="spermatogenesis-associated serine-rich protein
                     2-like isoform X1"
                     /calculated_mol_wt=63468
     Region          66..104
                     /region_name="CUE"
                     /note="CUE domain; pfam02845"
                     /db_xref="CDD:427018"
     Region          177..412
                     /region_name="DUF1387"
                     /note="Protein of unknown function (DUF1387); pfam07139"
                     /db_xref="CDD:462100"
     Region          <282..547
                     /region_name="CwlO1"
                     /note="Uncharacterized N-terminal coiled-coil domain of
                     peptidoglycan hydrolase CwlO [Function unknown]; COG3883"
                     /db_xref="CDD:443091"
     CDS             1..575
                     /gene="LOC144113142"
                     /coded_by="XM_077646055.1:67..1794"
                     /db_xref="GeneID:144113142"
ORIGIN      
        1 marklgggsr wlyekdaees cilpgpyaaq artwrraenk mrsiydthnn valadgeqhg
       61 pgdlkeklsk vrevvpsmsk dqicmvlrty dydvnatita isengpeaal rewncagnkv
      121 klanknrkrk kkpkkepaag smvenqdaia agdskdvnlg kldegkdhle ehfscished
      181 vpvssfkqng semekysgvs ygwgdepppe plhedeakhe vaeieskssr plhshgsgrs
      241 ksspdvkcgd gyevsatpss stlpkktcle kslkdlsrqt valqrvqvll eeqltkaeka
      301 ikaaflevqk alsdrqaqle eemekvrtda ramlqqrqkl aaelkarset astlseqslt
      361 elrddikhfv verkydeelg ktarfvcske kiekeikdfg qvvhvrdpyt srrlslssva
      421 sstyhdsgyh qsvtsspvyk sqyysydtmk eeehggrkfe gaggysrssd srgpdtrask
      481 vytnshrnay pgsspggars ggyprvgrnr rpddmrysgs drgggggrgg yggggrphry
      541 ststgrgryp tetgrrsred dgtrkpsptg vngqa
//
LOCUS       XP_077502182             574 aa            linear   INV 25-NOV-2025
DEFINITION  spermatogenesis-associated serine-rich protein 2-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077502182
VERSION     XP_077502182.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646056.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..574
                     /product="spermatogenesis-associated serine-rich protein
                     2-like isoform X2"
                     /calculated_mol_wt=63380
     Region          66..104
                     /region_name="CUE"
                     /note="CUE domain; pfam02845"
                     /db_xref="CDD:427018"
     Region          <207..411
                     /region_name="DUF1387"
                     /note="Protein of unknown function (DUF1387); pfam07139"
                     /db_xref="CDD:462100"
     Region          <281..546
                     /region_name="CwlO1"
                     /note="Uncharacterized N-terminal coiled-coil domain of
                     peptidoglycan hydrolase CwlO [Function unknown]; COG3883"
                     /db_xref="CDD:443091"
     CDS             1..574
                     /gene="LOC144113142"
                     /coded_by="XM_077646056.1:67..1791"
                     /db_xref="GeneID:144113142"
ORIGIN      
        1 marklgggsr wlyekdaees cilpgpyaaq artwrraenk mrsiydthnn valadgeqhg
       61 pgdlkeklsk vrevvpsmsk dqicmvlrty dydvnatita isengpeaal rewncagnkv
      121 klanknrkrk kkpkkepaag smvenqdaia agdskdvnlg kldegkdhle ehfscished
      181 vpvssfkqng semekysgvs ygwgdepppe plhedeakhe vaeiesksrp lhshgsgrsk
      241 sspdvkcgdg yevsatpsss tlpkktclek slkdlsrqtv alqrvqvlle eqltkaekai
      301 kaaflevqka lsdrqaqlee emekvrtdar amlqqrqkla aelkarseta stlseqslte
      361 lrddikhfvv erkydeelgk tarfvcskek iekeikdfgq vvhvrdpyts rrlslssvas
      421 styhdsgyhq svtsspvyks qyysydtmke eehggrkfeg aggysrssds rgpdtraskv
      481 ytnshrnayp gsspggarsg gyprvgrnrr pddmrysgsd rgggggrggy ggggrphrys
      541 tstgrgrypt etgrrsredd gtrkpsptgv ngqa
//
LOCUS       XP_077502183             541 aa            linear   INV 25-NOV-2025
DEFINITION  spermatogenesis-associated serine-rich protein 2-like isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077502183
VERSION     XP_077502183.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646057.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..541
                     /product="spermatogenesis-associated serine-rich protein
                     2-like isoform X3"
                     /calculated_mol_wt=59543
     Region          32..70
                     /region_name="CUE"
                     /note="CUE domain; pfam02845"
                     /db_xref="CDD:427018"
     Region          143..378
                     /region_name="DUF1387"
                     /note="Protein of unknown function (DUF1387); pfam07139"
                     /db_xref="CDD:462100"
     Region          <248..513
                     /region_name="CwlO1"
                     /note="Uncharacterized N-terminal coiled-coil domain of
                     peptidoglycan hydrolase CwlO [Function unknown]; COG3883"
                     /db_xref="CDD:443091"
     CDS             1..541
                     /gene="LOC144113142"
                     /coded_by="XM_077646057.1:77..1702"
                     /db_xref="GeneID:144113142"
ORIGIN      
        1 marklgvrsi ydthnnvala dgeqhgpgdl keklskvrev vpsmskdqic mvlrtydydv
       61 natitaisen gpeaalrewn cagnkvklan knrkrkkkpk kepaagsmve nqdaiaagds
      121 kdvnlgklde gkdhleehfs cishedvpvs sfkqngseme kysgvsygwg depppeplhe
      181 deakhevaei eskssrplhs hgsgrskssp dvkcgdgyev satpssstlp kktclekslk
      241 dlsrqtvalq rvqvlleeql tkaekaikaa flevqkalsd rqaqleeeme kvrtdaraml
      301 qqrqklaael karsetastl seqsltelrd dikhfvverk ydeelgktar fvcskekiek
      361 eikdfgqvvh vrdpytsrrl slssvassty hdsgyhqsvt sspvyksqyy sydtmkeeeh
      421 ggrkfegagg ysrssdsrgp dtraskvytn shrnaypgss pggarsggyp rvgrnrrpdd
      481 mrysgsdrgg gggrggyggg grphrystst grgryptetg rrsreddgtr kpsptgvngq
      541 a
//
LOCUS       XP_077502184             512 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113142 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077502184
VERSION     XP_077502184.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646058.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..512
                     /product="uncharacterized protein LOC144113142 isoform X4"
                     /calculated_mol_wt=56431
     Region          114..349
                     /region_name="DUF1387"
                     /note="Protein of unknown function (DUF1387); pfam07139"
                     /db_xref="CDD:462100"
     Region          <219..484
                     /region_name="CwlO1"
                     /note="Uncharacterized N-terminal coiled-coil domain of
                     peptidoglycan hydrolase CwlO [Function unknown]; COG3883"
                     /db_xref="CDD:443091"
     CDS             1..512
                     /gene="LOC144113142"
                     /coded_by="XM_077646058.1:67..1605"
                     /db_xref="GeneID:144113142"
ORIGIN      
        1 marklgggsr wlyekdaees cilpgpyaaq artwrraenk ngpeaalrew ncagnkvkla
       61 nknrkrkkkp kkepaagsmv enqdaiaagd skdvnlgkld egkdhleehf scishedvpv
      121 ssfkqngsem ekysgvsygw gdepppeplh edeakhevae ieskssrplh shgsgrskss
      181 pdvkcgdgye vsatpssstl pkktcleksl kdlsrqtval qrvqvlleeq ltkaekaika
      241 aflevqkals drqaqleeem ekvrtdaram lqqrqklaae lkarsetast lseqsltelr
      301 ddikhfvver kydeelgkta rfvcskekie keikdfgqvv hvrdpytsrr lslssvasst
      361 yhdsgyhqsv tsspvyksqy ysydtmkeee hggrkfegag gysrssdsrg pdtraskvyt
      421 nshrnaypgs spggarsggy prvgrnrrpd dmrysgsdrg ggggrggygg ggrphrysts
      481 tgrgryptet grrsreddgt rkpsptgvng qa
//
LOCUS       XP_077502185             511 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113142 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077502185
VERSION     XP_077502185.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646059.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..511
                     /product="uncharacterized protein LOC144113142 isoform X5"
                     /calculated_mol_wt=56343
     Region          <144..348
                     /region_name="DUF1387"
                     /note="Protein of unknown function (DUF1387); pfam07139"
                     /db_xref="CDD:462100"
     Region          <218..483
                     /region_name="CwlO1"
                     /note="Uncharacterized N-terminal coiled-coil domain of
                     peptidoglycan hydrolase CwlO [Function unknown]; COG3883"
                     /db_xref="CDD:443091"
     CDS             1..511
                     /gene="LOC144113142"
                     /coded_by="XM_077646059.1:67..1602"
                     /db_xref="GeneID:144113142"
ORIGIN      
        1 marklgggsr wlyekdaees cilpgpyaaq artwrraenk ngpeaalrew ncagnkvkla
       61 nknrkrkkkp kkepaagsmv enqdaiaagd skdvnlgkld egkdhleehf scishedvpv
      121 ssfkqngsem ekysgvsygw gdepppeplh edeakhevae iesksrplhs hgsgrskssp
      181 dvkcgdgyev satpssstlp kktclekslk dlsrqtvalq rvqvlleeql tkaekaikaa
      241 flevqkalsd rqaqleeeme kvrtdaraml qqrqklaael karsetastl seqsltelrd
      301 dikhfvverk ydeelgktar fvcskekiek eikdfgqvvh vrdpytsrrl slssvassty
      361 hdsgyhqsvt sspvyksqyy sydtmkeeeh ggrkfegagg ysrssdsrgp dtraskvytn
      421 shrnaypgss pggarsggyp rvgrnrrpdd mrysgsdrgg gggrggyggg grphrystst
      481 grgryptetg rrsreddgtr kpsptgvngq a
//
LOCUS       XP_077502186             478 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113142 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077502186
VERSION     XP_077502186.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646060.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..478
                     /product="uncharacterized protein LOC144113142 isoform X6"
                     /calculated_mol_wt=52539
     Region          80..315
                     /region_name="DUF1387"
                     /note="Protein of unknown function (DUF1387); pfam07139"
                     /db_xref="CDD:462100"
     Region          <185..450
                     /region_name="CwlO1"
                     /note="Uncharacterized N-terminal coiled-coil domain of
                     peptidoglycan hydrolase CwlO [Function unknown]; COG3883"
                     /db_xref="CDD:443091"
     CDS             1..478
                     /gene="LOC144113142"
                     /coded_by="XM_077646060.1:67..1503"
                     /db_xref="GeneID:144113142"
ORIGIN      
        1 marklgdgpe aalrewncag nkvklanknr krkkkpkkep aagsmvenqd aiaagdskdv
       61 nlgkldegkd hleehfscis hedvpvssfk qngsemekys gvsygwgdep ppeplhedea
      121 khevaeiesk ssrplhshgs grsksspdvk cgdgyevsat pssstlpkkt clekslkdls
      181 rqtvalqrvq vlleeqltka ekaikaafle vqkalsdrqa qleeemekvr tdaramlqqr
      241 qklaaelkar setastlseq sltelrddik hfvverkyde elgktarfvc skekiekeik
      301 dfgqvvhvrd pytsrrlsls svasstyhds gyhqsvtssp vyksqyysyd tmkeeehggr
      361 kfegaggysr ssdsrgpdtr askvytnshr naypgsspgg arsggyprvg rnrrpddmry
      421 sgsdrggggg rggyggggrp hryststgrg ryptetgrrs reddgtrkps ptgvngqa
//
LOCUS       XP_077502187             810 aa            linear   INV 25-NOV-2025
DEFINITION  armadillo [Amblyomma americanum].
ACCESSION   XP_077502187
VERSION     XP_077502187.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646061.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..810
                     /product="armadillo"
                     /calculated_mol_wt=88247
     Region          88..162
                     /region_name="CTNNAbd_dArm"
                     /note="alpha-catenin binding domain found in Drosophila
                     melanogaster armadillo segment polarity protein (dArm) and
                     similar proteins; cd21726"
                     /db_xref="CDD:439243"
     Site            order(94..97,101..102,104..106,109..110,113,131..133,
                     135..136,139..140,143,146..154)
                     /site_type="other"
                     /note="putative CNTTA binding site [polypeptide binding]"
                     /db_xref="CDD:439243"
     Region          164..>676
                     /region_name="PLN03200"
                     /note="cellulose synthase-interactive protein;
                     Provisional"
                     /db_xref="CDD:215629"
     Region          164..189
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          196..234
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          241..273
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          241..273
                     /region_name="Arm"
                     /note="Armadillo/beta-catenin-like repeat; pfam00514"
                     /db_xref="CDD:425727"
     Site            order(264,268,272,306,310,314,349,353,357)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293788"
     Region          281..317
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          323..358
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          365..400
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          413..438
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Site            order(429,433,437,473,477,481,519,523,527)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293788"
     Region          446..484
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          493..528
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          535..596
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          600..638
                     /region_name="Arm"
                     /note="Armadillo/beta-catenin-like repeat; pfam00514"
                     /db_xref="CDD:425727"
     Region          602..636
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          643..677
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     CDS             1..810
                     /gene="arm"
                     /coded_by="XM_077646061.1:153..2585"
                     /db_xref="GeneID:144113143"
ORIGIN      
        1 msyqmqsgaq patrsmshgn ysnpgdvpvn pkeqtilwqq gnyladsgih sgvttqtpsi
       61 tgkeddlgde qmvfdweqpg fgqgftpdqv demnqqlsqt rsqrvraamf petldegiei
      121 pstqydpaqp tavqrlseps qmlkhavvnl inyqddadla traipelikl lndedqvvvs
      181 qaammvhqls kkeasrhaim nspqmvaalv ramstsndle ttrcaagtlh nlshhrqgll
      241 aifksggipa lvkllsspve svlfyaittl hnlllhqegs kmavrlaggl qkmvsllqrn
      301 nvkflaivtd clqilaygnq eskliilasg gpaelvrimr sytyekllwt tsrvlkvlsv
      361 cssnkpaive aggvqatsmh lghqsqrlvl nclwtlrnls daalkqesle pllaslvqll
      421 asgdinvvtc aagilsnltc nnhrnkvtlc hvggiealvr tviqagdree itepavcalr
      481 hltcrhpeae laqnsvrlny glqvivkllh ppsrwplvka viglirnlal cpanhaplre
      541 hgaiprlvql lhksyqdtqr qrssvasngs qqgaysdgvr meeivegtvg alhilareah
      601 nraiirglnv ipvfvqllys dieniqrvaa gvlcelaadk egaemieadg atapltdllh
      661 srnegvatya aavlfrmsed kpqdykkrls meltnslfre dagpwagtgp dmdmnldayh
      721 eqmysgsqgp psgrhnpysq qgydqqvpid smqgldlgps qgghnytpmd tgmdldapgd
      781 lnfdpldpsl papaqqdssg qlaawydtdl
//
LOCUS       XP_077502188             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113145 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502188
VERSION     XP_077502188.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646062.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144113145 isoform X1"
                     /calculated_mol_wt=12677
     Region          <8..109
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..127
                     /gene="LOC144113145"
                     /coded_by="XM_077646062.1:444..827"
                     /db_xref="GeneID:144113145"
ORIGIN      
        1 msasgsspta lptganspvv elppvspdit sdsgtsgars npasspfsaa rddllapisa
       61 pdqtvpppda prdvegpgac gspqrppsls ddlpvrgsss gepptfpapi cifayhlyrr
      121 pssclea
//
LOCUS       XP_077502189             110 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113145 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502189
VERSION     XP_077502189.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646063.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..110
                     /product="uncharacterized protein LOC144113145 isoform X2"
                     /calculated_mol_wt=10614
     Region          <19..>110
                     /region_name="PRK12323"
                     /note="DNA polymerase III subunit gamma/tau; cl46901"
                     /db_xref="CDD:481241"
     CDS             1..110
                     /gene="LOC144113145"
                     /coded_by="XM_077646063.1:441..773"
                     /db_xref="GeneID:144113145"
ORIGIN      
        1 msasgsspta lptganspvv elppvspdit sdsgtsgars npasspfsaa rddllapisa
       61 pdqtvpppda prdvegpgac gspqrppsls ddlpvrgsss gepptfpasa
//
LOCUS       XP_077502190             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113147 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502190
VERSION     XP_077502190.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646064.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144113147 isoform X1"
                     /calculated_mol_wt=12691
     Region          <19..>108
                     /region_name="PRK12323"
                     /note="DNA polymerase III subunit gamma/tau; cl46901"
                     /db_xref="CDD:481241"
     CDS             1..127
                     /gene="LOC144113147"
                     /coded_by="XM_077646064.1:422..805"
                     /db_xref="GeneID:144113147"
ORIGIN      
        1 msasgsspta lptganspvv elppvspdit sdsgtsgsrt npasspfsaa rddllapisa
       61 pdqtvpppda prdvegpgac gspqrppsps ddlpvrgsss gepptfpapi cifayhlyrr
      121 pssclea
//
LOCUS       XP_077502191             110 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113147 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502191
VERSION     XP_077502191.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646065.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..110
                     /product="uncharacterized protein LOC144113147 isoform X2"
                     /calculated_mol_wt=10627
     Region          <19..>110
                     /region_name="PRK12323"
                     /note="DNA polymerase III subunit gamma/tau; cl46901"
                     /db_xref="CDD:481241"
     CDS             1..110
                     /gene="LOC144113147"
                     /coded_by="XM_077646065.1:422..754"
                     /db_xref="GeneID:144113147"
ORIGIN      
        1 msasgsspta lptganspvv elppvspdit sdsgtsgsrt npasspfsaa rddllapisa
       61 pdqtvpppda prdvegpgac gspqrppsps ddlpvrgsss gepptfpasa
//
LOCUS       XP_077502192             311 aa            linear   INV 25-NOV-2025
DEFINITION  RA domain-containing protein rau isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502192
VERSION     XP_077502192.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646066.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..311
                     /product="RA domain-containing protein rau isoform X1"
                     /calculated_mol_wt=35319
     Region          73..166
                     /region_name="Ubl1_cv_Nsp3_N-like"
                     /note="first ubiquitin-like (Ubl) domain located at the
                     N-terminus of coronavirus SARS-CoV non-structural protein
                     3 (Nsp3) and related proteins; cl28922"
                     /db_xref="CDD:475130"
     Region          169..254
                     /region_name="RA"
                     /note="Ras-associating (RA) domain, structurally similar
                     to a beta-grasp ubiquitin-like fold; cd17043"
                     /db_xref="CDD:340563"
     CDS             1..311
                     /gene="rau"
                     /coded_by="XM_077646066.1:124..1059"
                     /db_xref="GeneID:144113149"
ORIGIN      
        1 mlkhvhtstt tlspfrcikp qandalslns svsfgsggae qlssrsssts slhealpprk
       61 rspirsgede ltmvkvyarv lcadmeyktl cidttasssd vvrlllskfk mkhrdpnlfy
      121 ltmevwvrkt gipirtimvl ddearpvelq schpkgdskf llqmrrgglv kvydsclmag
      181 slyksllvse rttaeevvql vlhcynssdr gskyslyevc piknyeralh phelplriqq
      241 nwpspehfaf hlrrnqegth qkrqlpwtcq tdmtghlnfr lnvsrnelsp aaihsptlkp
      301 synnyencfy i
//
LOCUS       XP_077502193             300 aa            linear   INV 25-NOV-2025
DEFINITION  RA domain-containing protein rau isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502193
VERSION     XP_077502193.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646067.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..300
                     /product="RA domain-containing protein rau isoform X2"
                     /calculated_mol_wt=34078
     Region          60..155
                     /region_name="Ubl1_cv_Nsp3_N-like"
                     /note="first ubiquitin-like (Ubl) domain located at the
                     N-terminus of coronavirus SARS-CoV non-structural protein
                     3 (Nsp3) and related proteins; cl28922"
                     /db_xref="CDD:475130"
     Region          158..243
                     /region_name="RA"
                     /note="Ras-associating (RA) domain, structurally similar
                     to a beta-grasp ubiquitin-like fold; cd17043"
                     /db_xref="CDD:340563"
     CDS             1..300
                     /gene="rau"
                     /coded_by="XM_077646067.1:122..1024"
                     /db_xref="GeneID:144113149"
ORIGIN      
        1 mlkhvhtstt tlspfrcikp qandalslns svsfgsggae qlssrsssts slhealpprk
       61 tmvkvyarvl cadmeyktlc idttasssdv vrlllskfkm khrdpnlfyl tmevwvrktg
      121 ipirtimvld dearpvelqs chpkgdskfl lqmrrgglvk vydsclmags lyksllvser
      181 ttaeevvqlv lhcynssdrg skyslyevcp iknyeralhp helplriqqn wpspehfafh
      241 lrrnqegthq krqlpwtcqt dmtghlnfrl nvsrnelspa aihsptlkps ynnyencfyi
//
LOCUS       XP_077502194            1069 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113150 [Amblyomma americanum].
ACCESSION   XP_077502194
VERSION     XP_077502194.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646068.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1069
                     /product="uncharacterized protein LOC144113150"
                     /calculated_mol_wt=121854
     Region          <192..294
                     /region_name="PHA03309"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:165564"
     Region          311..331
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(311,314,327,331)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(316,318,320,322..323,326..327,330,346,348,352..353,
                     356..357,360,374,376,378,380..381,384..385,388)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          <332..415
                     /region_name="SFP1"
                     /note="Putative transcriptional repressor regulating G2/M
                     transition [Transcription / Cell division and chromosome
                     partitioning]; COG5189"
                     /db_xref="CDD:227516"
     Region          341..361
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          369..389
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          394..410
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          452..473
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          475..869
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          481..501
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(481,484,497,501)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(486,488,490,492..493,496..497,500,522,524,528..529,
                     532..533,536,550,552,554,556..557,560..561,564)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          517..537
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          545..565
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          573..594
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          602..619
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          643..663
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(643,646,659,663)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(648,650,652,654..655,658..659,662,676,678,682..683,
                     686..687,691,704,706,708,710..711,714..715)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          671..692
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          699..715
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          727..747
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          755..775
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          796..816
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          825..845
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(825,828,841,845)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(830,832,834,836..837,840..841,844,858,860,864..865,
                     868..869,872,887,889,891,893..894,897..898)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          853..874
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          882..900
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1069
                     /gene="LOC144113150"
                     /coded_by="XM_077646068.1:474..3683"
                     /db_xref="GeneID:144113150"
ORIGIN      
        1 metrvfinid vepfctklvf pvgvpaagyv riifgdfakt cskyravneg aadsesdast
       61 avndkvfvrs psidgeplct lksaalpddl nddptaaaae eeeeskevnd dnppqepdve
      121 eptepaelqs natpanelss drrkqknpmk irlkalpvap lpdttrrssr trnaatvryv
      181 sssssdddfn rdsadsdwsg sdesyekapk vkrtrrasap irrattagrk atslrkslpa
      241 kklspakkst mpsrkttasf kkslsvakas atetptpkva plnikalkqq srerqalkas
      301 nkaksdetyt ceecgqtfss rlkykkhswt hkpksehphr cdvcdklfat aftlerhqaa
      361 hckgmpncce kcrkgfatpe dlakheethk dyrceccsql ylsyrglrkh ytqfhkneml
      421 nkqqssgnat edgtsgkqge sensasqkeq kcakcdlgcd seealathym evhpdqqvfp
      481 cetcrkvftd rdmlqthlrk hmqiktgens seqtylcnic dkelkslstl rvhvqshnkv
      541 rsfkcdicqe kyttasnlrk hrkihnttrm ykcphcpkef ttnnyklkhv krvhtrdfqy
      601 gcshcpkkfl ekkrfqqhmv lmhasmlted dyatmtlvkk lhcsqcsyvt yssksfrqhe
      661 vthtgkypht csecnkgftf rfeltkhvrc vhqaqffrce sckrtftaee tykihkehhd
      721 rglgftctec gnmyetqghy eqhmqahskd lpyecptchk rfaltrslnl hrlshdkqrt
      781 prprpysgnr sweyycelcn vfykygsslv ahrvcvhgng ptttcpycdk kfnsrltlsm
      841 hirmhtrerp mrcsycgktf rvwnslkrhv vtshtkdfkl fcplcnkgfv srsylkvhlk
      901 sahkavmsgp gkrrrltdap taaqhppara vtvqkppvii pmpakldrst lvpqarrprh
      961 htakataaav ppapvathpp lvpppaqvdt sqrpqlqppr pqqvaverpq lqqqaqlqql
     1021 hlqqlqlqps qmhpqhlqpp rqqtqqqrdl femqqipqle sdtyslmll
//
LOCUS       XP_077502195             206 aa            linear   INV 25-NOV-2025
DEFINITION  receptor expression-enhancing protein 5-like [Amblyomma
            americanum].
ACCESSION   XP_077502195
VERSION     XP_077502195.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646069.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..206
                     /product="receptor expression-enhancing protein 5-like"
                     /calculated_mol_wt=23597
     Region          107..176
                     /region_name="TB2_DP1_HVA22"
                     /note="TB2/DP1, HVA22 family; pfam03134"
                     /db_xref="CDD:460820"
     CDS             1..206
                     /gene="LOC144113151"
                     /coded_by="XM_077646069.1:42..662"
                     /db_xref="GeneID:144113151"
ORIGIN      
        1 msppdgnrvd adsseeasts egkpsniams sppkkplicr ilhrfhcllr aqmyadtilg
       61 rsaaelqeas nikrefllyi lvillilsll igvfgrhvsn licvpypavv smisvdsnir
      121 qryvkwityw mvfgfvnvvd mmcplistiv pqyylwrtlf lawcfspykw ngckliresl
      181 ytsrnmrilr viayvfyeaa rmltvq
//
LOCUS       XP_077502196             111 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113152 [Amblyomma americanum].
ACCESSION   XP_077502196
VERSION     XP_077502196.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646070.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..111
                     /product="uncharacterized protein LOC144113152"
                     /calculated_mol_wt=11951
     CDS             1..111
                     /gene="LOC144113152"
                     /coded_by="XM_077646070.1:682..1017"
                     /db_xref="GeneID:144113152"
ORIGIN      
        1 mqanrlrtpp lrrihplssg ggspsgwsge gwtsiwakpa skgkqvmhpk tehkklthha
       61 rdntpsgpnr plmptdgsgl avllrsfthr mqqpqgsths hvctsadgly t
//
LOCUS       XP_077502197             329 aa            linear   INV 25-NOV-2025
DEFINITION  cyclin Y [Amblyomma americanum].
ACCESSION   XP_077502197
VERSION     XP_077502197.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646071.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..329
                     /product="cyclin Y"
                     /calculated_mol_wt=37646
     Region          152..248
                     /region_name="CYCLIN_CCNY_like"
                     /note="cyclin box found in cyclin-Y (CCNY) family;
                     cd20540"
                     /db_xref="CDD:410243"
     CDS             1..329
                     /gene="CycY"
                     /coded_by="XM_077646071.1:249..1238"
                     /db_xref="GeneID:144113153"
ORIGIN      
        1 mggslsccsa psprphrkkd sadpphsees asnlqhiser epedfdqdps thptagplfm
       61 qrsrsdvrnc kekrrsqinl qekaplkksn scstiyldds tvsqpnlrct ikcvtlaiyy
      121 hirdrggqqr tidifderlh plskeppemr dpdhrtiyrf vrtlfnaaql saecaiitlv
      181 ylerlltyae mdispcswrr vvlgavllas kvwddqavwn vdycqilkei tvedmneler
      241 qflellqfni nvpasvyaky yfdlrtladh nelafpsepl skeraqklea msrvcedklg
      301 elhrngfkkw ssldnvnnvs vrrstails
//
LOCUS       XP_077502198             329 aa            linear   INV 25-NOV-2025
DEFINITION  cyclin Y [Amblyomma americanum].
ACCESSION   XP_077502198
VERSION     XP_077502198.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646072.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..329
                     /product="cyclin Y"
                     /calculated_mol_wt=37646
     Region          152..248
                     /region_name="CYCLIN_CCNY_like"
                     /note="cyclin box found in cyclin-Y (CCNY) family;
                     cd20540"
                     /db_xref="CDD:410243"
     CDS             1..329
                     /gene="CycY"
                     /coded_by="XM_077646072.1:172..1161"
                     /db_xref="GeneID:144113153"
ORIGIN      
        1 mggslsccsa psprphrkkd sadpphsees asnlqhiser epedfdqdps thptagplfm
       61 qrsrsdvrnc kekrrsqinl qekaplkksn scstiyldds tvsqpnlrct ikcvtlaiyy
      121 hirdrggqqr tidifderlh plskeppemr dpdhrtiyrf vrtlfnaaql saecaiitlv
      181 ylerlltyae mdispcswrr vvlgavllas kvwddqavwn vdycqilkei tvedmneler
      241 qflellqfni nvpasvyaky yfdlrtladh nelafpsepl skeraqklea msrvcedklg
      301 elhrngfkkw ssldnvnnvs vrrstails
//
LOCUS       XP_077502199            1370 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113154 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502199
VERSION     XP_077502199.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646073.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1370
                     /product="uncharacterized protein LOC144113154 isoform X1"
                     /calculated_mol_wt=148641
     Region          599..669
                     /region_name="PDZ_5"
                     /note="PDZ domain; pfam17817"
                     /db_xref="CDD:465518"
     Region          673..762
                     /region_name="PDZ_neurabin-like"
                     /note="PDZ domain of neurabin-1 and neurabin-2, and
                     related domains; cd06790"
                     /db_xref="CDD:467252"
     Site            order(685..691,709,712,742,746,749..750)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467252"
     Region          <843..>982
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          1250..1319
                     /region_name="SAM_Neurabin-like"
                     /note="SAM domain of SAM_Neurabin-like subfamily; cd09512"
                     /db_xref="CDD:188911"
     Region          1309..>1348
                     /region_name="PRK11778"
                     /note="putative inner membrane peptidase; Provisional"
                     /db_xref="CDD:236978"
     CDS             1..1370
                     /gene="LOC144113154"
                     /coded_by="XM_077646073.1:199..4311"
                     /db_xref="GeneID:144113154"
ORIGIN      
        1 maevrrsevl aasrsrtgwl deppptsssp tspakpfiss lrstfesrtn qtqdgaerrr
       61 vaptgtktgs kvssianmfq arddcktasn eslartsple pntadaarps gpptltrsds
      121 hvarfynara mferlqeask kpqpvsgrrg svasspassp ssatpatsfp kwseegpade
      181 saqslvngds qhsaksstea lsdparngps saisngvtrp elpgicagkd vaaseleppv
      241 kvpqhkkpsa pprveslrrs sedgllqgel prppeeasll ppeppvppph glpvaeeahp
      301 alssaqssss csldsaages rnadtandrl dtlplknsvp tgsnkpqleh stlhlkedsp
      361 lctdpgette gdkswgpseg tdsakqplgs myarvhnskp plpskppvlh hasskhdscp
      421 plemeevphw pqqdyvghng iseppsnsvl lytlaaasgr stmllqqdnv chdkysnsnn
      481 sglkhaaget laeletrehe lfnveavins rsykgdpfgd inmdaqvdgs gvefmtqeea
      541 dkllsssrqq eilsdeeaqe vrrllvlrtp sptvaeeesr ppsvvvveee gvsyhvlpdg
      601 hffterpgla sddeeepypv pfqprsarvk fstepikvfs thsvddydrr ndevdpvsas
      661 aeyelekrie kmdvfpvelv kgpeglglsi igmgvgadag leklgifvkt itengaaykd
      721 nrirvndqii evdgkslvgv tqayaasvlr ntsglvrfli grerdstnse iaqliyqsiq
      781 adrerearlk gvgqeavpgs gldslastpd devdelapga sfelsndssd slspdadpdn
      841 irlrfketqy rlavaeaear svrerlslae gerrdlgrll egaqgrlqea eatgqalrle
      901 aaqqrqaahe lrerlaalea kyarakrllr dlqqrahear qreeyhlhql hekdheynal
      961 vkalkdrvil lencltetqk aagvpvqlpy dntmklltpq mkkrrppppa vplseldsdd
     1021 clrcelspde vsgdhrrstv errgagdafd ravphtelld staakakael aakgslanrq
     1081 ppsvsalkkq tstdlaspde qqdlntsins tgsetsihss shmsdtsnas dssptrplsq
     1141 qlgddfkptv lqwqnrghte ssgslgslds ppgrrvssls sptdsqqsls piptppphaq
     1201 kvtllthkki dgslprecgs ssslrsscsn qgessgcerr eevrphhwqs gpvdrwsvsq
     1261 vgqwlvvlgl eahvatfaaq ciggetllql dsarlkelgv vstsdrsllk kkikelrsql
     1321 dkerkaqeke lrareklqrk adkakrksqp atgtsclvvr rvlafaflma
//
LOCUS       XP_077502200            1370 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113154 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502200
VERSION     XP_077502200.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646074.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1370
                     /product="uncharacterized protein LOC144113154 isoform X1"
                     /calculated_mol_wt=148641
     Region          599..669
                     /region_name="PDZ_5"
                     /note="PDZ domain; pfam17817"
                     /db_xref="CDD:465518"
     Region          673..762
                     /region_name="PDZ_neurabin-like"
                     /note="PDZ domain of neurabin-1 and neurabin-2, and
                     related domains; cd06790"
                     /db_xref="CDD:467252"
     Site            order(685..691,709,712,742,746,749..750)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467252"
     Region          <843..>982
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          1250..1319
                     /region_name="SAM_Neurabin-like"
                     /note="SAM domain of SAM_Neurabin-like subfamily; cd09512"
                     /db_xref="CDD:188911"
     Region          1309..>1348
                     /region_name="PRK11778"
                     /note="putative inner membrane peptidase; Provisional"
                     /db_xref="CDD:236978"
     CDS             1..1370
                     /gene="LOC144113154"
                     /coded_by="XM_077646074.1:82..4194"
                     /db_xref="GeneID:144113154"
ORIGIN      
        1 maevrrsevl aasrsrtgwl deppptsssp tspakpfiss lrstfesrtn qtqdgaerrr
       61 vaptgtktgs kvssianmfq arddcktasn eslartsple pntadaarps gpptltrsds
      121 hvarfynara mferlqeask kpqpvsgrrg svasspassp ssatpatsfp kwseegpade
      181 saqslvngds qhsaksstea lsdparngps saisngvtrp elpgicagkd vaaseleppv
      241 kvpqhkkpsa pprveslrrs sedgllqgel prppeeasll ppeppvppph glpvaeeahp
      301 alssaqssss csldsaages rnadtandrl dtlplknsvp tgsnkpqleh stlhlkedsp
      361 lctdpgette gdkswgpseg tdsakqplgs myarvhnskp plpskppvlh hasskhdscp
      421 plemeevphw pqqdyvghng iseppsnsvl lytlaaasgr stmllqqdnv chdkysnsnn
      481 sglkhaaget laeletrehe lfnveavins rsykgdpfgd inmdaqvdgs gvefmtqeea
      541 dkllsssrqq eilsdeeaqe vrrllvlrtp sptvaeeesr ppsvvvveee gvsyhvlpdg
      601 hffterpgla sddeeepypv pfqprsarvk fstepikvfs thsvddydrr ndevdpvsas
      661 aeyelekrie kmdvfpvelv kgpeglglsi igmgvgadag leklgifvkt itengaaykd
      721 nrirvndqii evdgkslvgv tqayaasvlr ntsglvrfli grerdstnse iaqliyqsiq
      781 adrerearlk gvgqeavpgs gldslastpd devdelapga sfelsndssd slspdadpdn
      841 irlrfketqy rlavaeaear svrerlslae gerrdlgrll egaqgrlqea eatgqalrle
      901 aaqqrqaahe lrerlaalea kyarakrllr dlqqrahear qreeyhlhql hekdheynal
      961 vkalkdrvil lencltetqk aagvpvqlpy dntmklltpq mkkrrppppa vplseldsdd
     1021 clrcelspde vsgdhrrstv errgagdafd ravphtelld staakakael aakgslanrq
     1081 ppsvsalkkq tstdlaspde qqdlntsins tgsetsihss shmsdtsnas dssptrplsq
     1141 qlgddfkptv lqwqnrghte ssgslgslds ppgrrvssls sptdsqqsls piptppphaq
     1201 kvtllthkki dgslprecgs ssslrsscsn qgessgcerr eevrphhwqs gpvdrwsvsq
     1261 vgqwlvvlgl eahvatfaaq ciggetllql dsarlkelgv vstsdrsllk kkikelrsql
     1321 dkerkaqeke lrareklqrk adkakrksqp atgtsclvvr rvlafaflma
//
LOCUS       XP_077502201            1347 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113154 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502201
VERSION     XP_077502201.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646075.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1347
                     /product="uncharacterized protein LOC144113154 isoform X2"
                     /calculated_mol_wt=146220
     Region          599..669
                     /region_name="PDZ_5"
                     /note="PDZ domain; pfam17817"
                     /db_xref="CDD:465518"
     Region          673..762
                     /region_name="PDZ_neurabin-like"
                     /note="PDZ domain of neurabin-1 and neurabin-2, and
                     related domains; cd06790"
                     /db_xref="CDD:467252"
     Site            order(685..691,709,712,742,746,749..750)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467252"
     Region          <843..>982
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          1250..1319
                     /region_name="SAM_Neurabin-like"
                     /note="SAM domain of SAM_Neurabin-like subfamily; cd09512"
                     /db_xref="CDD:188911"
     Region          1309..>1347
                     /region_name="PRK11778"
                     /note="putative inner membrane peptidase; Provisional"
                     /db_xref="CDD:236978"
     CDS             1..1347
                     /gene="LOC144113154"
                     /coded_by="XM_077646075.1:199..4242"
                     /db_xref="GeneID:144113154"
ORIGIN      
        1 maevrrsevl aasrsrtgwl deppptsssp tspakpfiss lrstfesrtn qtqdgaerrr
       61 vaptgtktgs kvssianmfq arddcktasn eslartsple pntadaarps gpptltrsds
      121 hvarfynara mferlqeask kpqpvsgrrg svasspassp ssatpatsfp kwseegpade
      181 saqslvngds qhsaksstea lsdparngps saisngvtrp elpgicagkd vaaseleppv
      241 kvpqhkkpsa pprveslrrs sedgllqgel prppeeasll ppeppvppph glpvaeeahp
      301 alssaqssss csldsaages rnadtandrl dtlplknsvp tgsnkpqleh stlhlkedsp
      361 lctdpgette gdkswgpseg tdsakqplgs myarvhnskp plpskppvlh hasskhdscp
      421 plemeevphw pqqdyvghng iseppsnsvl lytlaaasgr stmllqqdnv chdkysnsnn
      481 sglkhaaget laeletrehe lfnveavins rsykgdpfgd inmdaqvdgs gvefmtqeea
      541 dkllsssrqq eilsdeeaqe vrrllvlrtp sptvaeeesr ppsvvvveee gvsyhvlpdg
      601 hffterpgla sddeeepypv pfqprsarvk fstepikvfs thsvddydrr ndevdpvsas
      661 aeyelekrie kmdvfpvelv kgpeglglsi igmgvgadag leklgifvkt itengaaykd
      721 nrirvndqii evdgkslvgv tqayaasvlr ntsglvrfli grerdstnse iaqliyqsiq
      781 adrerearlk gvgqeavpgs gldslastpd devdelapga sfelsndssd slspdadpdn
      841 irlrfketqy rlavaeaear svrerlslae gerrdlgrll egaqgrlqea eatgqalrle
      901 aaqqrqaahe lrerlaalea kyarakrllr dlqqrahear qreeyhlhql hekdheynal
      961 vkalkdrvil lencltetqk aagvpvqlpy dntmklltpq mkkrrppppa vplseldsdd
     1021 clrcelspde vsgdhrrstv errgagdafd ravphtelld staakakael aakgslanrq
     1081 ppsvsalkkq tstdlaspde qqdlntsins tgsetsihss shmsdtsnas dssptrplsq
     1141 qlgddfkptv lqwqnrghte ssgslgslds ppgrrvssls sptdsqqsls piptppphaq
     1201 kvtllthkki dgslprecgs ssslrsscsn qgessgcerr eevrphhwqs gpvdrwsvsq
     1261 vgqwlvvlgl eahvatfaaq ciggetllql dsarlkelgv vstsdrsllk kkikelrsql
     1321 dkerkaqeke lrareklqrk adkakrk
//
LOCUS       XP_077502202            1311 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113154 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502202
VERSION     XP_077502202.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646076.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1311
                     /product="uncharacterized protein LOC144113154 isoform X3"
                     /calculated_mol_wt=142490
     Region          599..669
                     /region_name="PDZ_5"
                     /note="PDZ domain; pfam17817"
                     /db_xref="CDD:465518"
     Region          673..762
                     /region_name="PDZ_neurabin-like"
                     /note="PDZ domain of neurabin-1 and neurabin-2, and
                     related domains; cd06790"
                     /db_xref="CDD:467252"
     Site            order(685..691,709,712,742,746,749..750)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467252"
     Region          <843..>982
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          1191..1260
                     /region_name="SAM_Neurabin-like"
                     /note="SAM domain of SAM_Neurabin-like subfamily; cd09512"
                     /db_xref="CDD:188911"
     Region          1250..>1289
                     /region_name="PRK11778"
                     /note="putative inner membrane peptidase; Provisional"
                     /db_xref="CDD:236978"
     CDS             1..1311
                     /gene="LOC144113154"
                     /coded_by="XM_077646076.1:199..4134"
                     /db_xref="GeneID:144113154"
ORIGIN      
        1 maevrrsevl aasrsrtgwl deppptsssp tspakpfiss lrstfesrtn qtqdgaerrr
       61 vaptgtktgs kvssianmfq arddcktasn eslartsple pntadaarps gpptltrsds
      121 hvarfynara mferlqeask kpqpvsgrrg svasspassp ssatpatsfp kwseegpade
      181 saqslvngds qhsaksstea lsdparngps saisngvtrp elpgicagkd vaaseleppv
      241 kvpqhkkpsa pprveslrrs sedgllqgel prppeeasll ppeppvppph glpvaeeahp
      301 alssaqssss csldsaages rnadtandrl dtlplknsvp tgsnkpqleh stlhlkedsp
      361 lctdpgette gdkswgpseg tdsakqplgs myarvhnskp plpskppvlh hasskhdscp
      421 plemeevphw pqqdyvghng iseppsnsvl lytlaaasgr stmllqqdnv chdkysnsnn
      481 sglkhaaget laeletrehe lfnveavins rsykgdpfgd inmdaqvdgs gvefmtqeea
      541 dkllsssrqq eilsdeeaqe vrrllvlrtp sptvaeeesr ppsvvvveee gvsyhvlpdg
      601 hffterpgla sddeeepypv pfqprsarvk fstepikvfs thsvddydrr ndevdpvsas
      661 aeyelekrie kmdvfpvelv kgpeglglsi igmgvgadag leklgifvkt itengaaykd
      721 nrirvndqii evdgkslvgv tqayaasvlr ntsglvrfli grerdstnse iaqliyqsiq
      781 adrerearlk gvgqeavpgs gldslastpd devdelapga sfelsndssd slspdadpdn
      841 irlrfketqy rlavaeaear svrerlslae gerrdlgrll egaqgrlqea eatgqalrle
      901 aaqqrqaahe lrerlaalea kyarakrllr dlqqrahear qreeyhlhql hekdheynal
      961 vkalkdrvil lencltetqk aagvpvqlpy dntmklltpq mkkrrppppa vplseldsdd
     1021 clrcelspde vsgdhrrstv errgagdafd ravphtelld staakakael aakgslanrq
     1081 ppsvsalkkq tstdlaspde qqdlntsins tgsetsihss shmsdtsnas dssptrplsq
     1141 qlgddfkptv lqwqnrgecg sssslrsscs nqgessgcer reevrphhwq sgpvdrwsvs
     1201 qvgqwlvvlg leahvatfaa qciggetllq ldsarlkelg vvstsdrsll kkkikelrsq
     1261 ldkerkaqek elrareklqr kadkakrksq patgtsclvv rrvlafaflm a
//
LOCUS       XP_077502203             623 aa            linear   INV 25-NOV-2025
DEFINITION  ecdysone-induced protein 75B isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502203
VERSION     XP_077502203.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646077.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..623
                     /product="ecdysone-induced protein 75B isoform X1"
                     /calculated_mol_wt=68848
     Region          34..122
                     /region_name="NR_DBD_REV_ERB"
                     /note="DNA-binding domain of REV-ERB receptor-like is
                     composed of two C4-type zinc fingers; cd07166"
                     /db_xref="CDD:143540"
     Site            order(39,42,56,59,76,82,92,95)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143540"
     Site            order(48..51,57..58,60,62,64..65,89..90,93,96,107,
                     110..116)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143540"
     Site            order(107..108,112)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143540"
     Region          190..384
                     /region_name="NR_LBD"
                     /note="The ligand binding domain of nuclear receptors, a
                     family of ligand-activated transcription regulators;
                     cl11397"
                     /db_xref="CDD:472173"
     Site            order(211..212,215,218..219,248..249,252..253,256,259,293)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132738"
     Site            order(220,223,227,232,237..238,240..241,244..245)
                     /site_type="active"
                     /note="coregulator recognition site [active]"
                     /db_xref="CDD:132738"
     CDS             1..623
                     /gene="Eip75B"
                     /coded_by="XM_077646077.1:190..2061"
                     /db_xref="GeneID:144113155"
ORIGIN      
        1 miltekdlql latlpfqqqq lerkdadlki efdgttvlcr vcgdkasgfh ygvhscegck
       61 gffrrsiqqk iqyrpctknq qcsilrinrn rcqycrlkkc iavgmsrdav rfgrvpkrek
      121 akilaamqkv nassqekala veledemrlf etvvraheet cdftsdkvsp llerarahpv
      181 ytlcppqlac plnpttqpae gsgaprlmed fserfspair gvvefakrip gfsllaqddq
      241 vtllkagvfe vllvrlacmf dsrsssmvcl ngqvllresl hsasnarfll dsmfdfadrl
      301 nalgltdael glfcsvvvia pdrpglrntd lvqkmqrrls evlqkavaag hpdrpqlcae
      361 llkkvpdlrt lntlhsekll aykmepatpp yleqwgated wspasphsva sstnsldgak
      421 spvrsgsfss stdyspdspa kpfakvrrld sptdsgidsg kepgcctpsa svcssprsld
      481 dkaadepdra dedmpvlkra lqapplvsph qlmeeayrhh kklravrkea asssadlass
      541 hstlvgrlqq aprflneqql krtdlihnii mrnegpqnff qgwgspprcp yspqpspplv
      601 pqdgpqplnl skkpptpplk tet
//
LOCUS       XP_077502204             612 aa            linear   INV 25-NOV-2025
DEFINITION  ecdysone-induced protein 75B isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502204
VERSION     XP_077502204.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646078.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..612
                     /product="ecdysone-induced protein 75B isoform X2"
                     /calculated_mol_wt=67293
     Region          23..111
                     /region_name="NR_DBD_REV_ERB"
                     /note="DNA-binding domain of REV-ERB receptor-like is
                     composed of two C4-type zinc fingers; cd07166"
                     /db_xref="CDD:143540"
     Site            order(28,31,45,48,65,71,81,84)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143540"
     Site            order(37..40,46..47,49,51,53..54,78..79,82,85,96,99..105)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143540"
     Site            order(96..97,101)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143540"
     Region          179..373
                     /region_name="NR_LBD"
                     /note="The ligand binding domain of nuclear receptors, a
                     family of ligand-activated transcription regulators;
                     cl11397"
                     /db_xref="CDD:472173"
     Site            order(200..201,204,207..208,237..238,241..242,245,248,282)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132726"
     Site            order(209,212,216,221,226..227,229..230,233..234)
                     /site_type="active"
                     /note="coregulator recognition site [active]"
                     /db_xref="CDD:132726"
     CDS             1..612
                     /gene="Eip75B"
                     /coded_by="XM_077646078.1:293..2131"
                     /db_xref="GeneID:144113155"
ORIGIN      
        1 mvqgllqakp aepphegaie fdgttvlcrv cgdkasgfhy gvhscegckg ffrrsiqqki
       61 qyrpctknqq csilrinrnr cqycrlkkci avgmsrdavr fgrvpkreka kilaamqkvn
      121 assqekalav eledemrlfe tvvraheetc dftsdkvspl lerarahpvy tlcppqlacp
      181 lnpttqpaeg sgaprlmedf serfspairg vvefakripg fsllaqddqv tllkagvfev
      241 llvrlacmfd srsssmvcln gqvllreslh sasnarflld smfdfadrln algltdaelg
      301 lfcsvvviap drpglrntdl vqkmqrrlse vlqkavaagh pdrpqlcael lkkvpdlrtl
      361 ntlhseklla ykmepatppy leqwgatedw spasphsvas stnsldgaks pvrsgsfsss
      421 tdyspdspak pfakvrrlds ptdsgidsgk epgcctpsas vcssprsldd kaadepdrad
      481 edmpvlkral qapplvsphq lmeeayrhhk klravrkeaa sssadlassh stlvgrlqqa
      541 prflneqqlk rtdlihniim rnegpqnffq gwgspprcpy spqpspplvp qdgpqplnls
      601 kkpptpplkt et
//
LOCUS       XP_077502205             536 aa            linear   INV 25-NOV-2025
DEFINITION  ecdysone-induced protein 75B isoform X3 [Amblyomma americanum].
ACCESSION   XP_077502205
VERSION     XP_077502205.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646079.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..536
                     /product="ecdysone-induced protein 75B isoform X3"
                     /calculated_mol_wt=58683
     Region          103..297
                     /region_name="NR_LBD"
                     /note="The ligand binding domain of nuclear receptors, a
                     family of ligand-activated transcription regulators;
                     cl11397"
                     /db_xref="CDD:472173"
     Site            order(124..125,128,131..132,161..162,165..166,169,172,206)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132726"
     Site            order(133,136,140,145,150..151,153..154,157..158)
                     /site_type="active"
                     /note="coregulator recognition site [active]"
                     /db_xref="CDD:132726"
     CDS             1..536
                     /gene="Eip75B"
                     /coded_by="XM_077646079.1:134..1744"
                     /db_xref="GeneID:144113155"
ORIGIN      
        1 mmmapdprpg nagylyfvdg kpvrfgrvpk rekakilaam qkvnassqek alaveledem
       61 rlfetvvrah eetcdftsdk vspllerara hpvytlcppq lacplnpttq paegsgaprl
      121 medfserfsp airgvvefak ripgfsllaq ddqvtllkag vfevllvrla cmfdsrsssm
      181 vclngqvllr eslhsasnar flldsmfdfa drlnalgltd aelglfcsvv viapdrpglr
      241 ntdlvqkmqr rlsevlqkav aaghpdrpql caellkkvpd lrtlntlhse kllaykmepa
      301 tppyleqwga tedwspasph svasstnsld gakspvrsgs fssstdyspd spakpfakvr
      361 rldsptdsgi dsgkepgcct psasvcsspr slddkaadep dradedmpvl kralqapplv
      421 sphqlmeeay rhhkklravr keaasssadl asshstlvgr lqqaprflne qqlkrtdlih
      481 niimrnegpq nffqgwgspp rcpyspqpsp plvpqdgpqp lnlskkpptp plktet
//
LOCUS       XP_077502206             519 aa            linear   INV 25-NOV-2025
DEFINITION  ecdysone-induced protein 75B isoform X4 [Amblyomma americanum].
ACCESSION   XP_077502206
VERSION     XP_077502206.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646080.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..519
                     /product="ecdysone-induced protein 75B isoform X4"
                     /calculated_mol_wt=56834
     Region          <1..18
                     /region_name="NR_DBD_like"
                     /note="DNA-binding domain of nuclear receptors is composed
                     of two C4-type zinc fingers; cl02596"
                     /db_xref="CDD:413390"
     Region          86..280
                     /region_name="NR_LBD"
                     /note="The ligand binding domain of nuclear receptors, a
                     family of ligand-activated transcription regulators;
                     cl11397"
                     /db_xref="CDD:472173"
     Site            order(107..108,111,114..115,144..145,148..149,152,155,189)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132726"
     Site            order(116,119,123,128,133..134,136..137,140..141)
                     /site_type="active"
                     /note="coregulator recognition site [active]"
                     /db_xref="CDD:132726"
     CDS             1..519
                     /gene="Eip75B"
                     /coded_by="XM_077646080.1:282..1841"
                     /db_xref="GeneID:144113155"
ORIGIN      
        1 msrdavrfgr vpkrekakil aamqkvnass qekalavele demrlfetvv raheetcdft
       61 sdkvspller arahpvytlc ppqlacplnp ttqpaegsga prlmedfser fspairgvve
      121 fakripgfsl laqddqvtll kagvfevllv rlacmfdsrs ssmvclngqv llreslhsas
      181 narflldsmf dfadrlnalg ltdaelglfc svvviapdrp glrntdlvqk mqrrlsevlq
      241 kavaaghpdr pqlcaellkk vpdlrtlntl hsekllaykm epatppyleq wgatedwspa
      301 sphsvasstn sldgakspvr sgsfssstdy spdspakpfa kvrrldsptd sgidsgkepg
      361 cctpsasvcs sprslddkaa depdradedm pvlkralqap plvsphqlme eayrhhkklr
      421 avrkeaasss adlasshstl vgrlqqaprf lneqqlkrtd lihniimrne gpqnffqgwg
      481 spprcpyspq pspplvpqdg pqplnlskkp ptpplktet
//
LOCUS       XP_077502207             519 aa            linear   INV 25-NOV-2025
DEFINITION  ecdysone-induced protein 75B isoform X4 [Amblyomma americanum].
ACCESSION   XP_077502207
VERSION     XP_077502207.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646081.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..519
                     /product="ecdysone-induced protein 75B isoform X4"
                     /calculated_mol_wt=56834
     Region          <1..18
                     /region_name="NR_DBD_like"
                     /note="DNA-binding domain of nuclear receptors is composed
                     of two C4-type zinc fingers; cl02596"
                     /db_xref="CDD:413390"
     Region          86..280
                     /region_name="NR_LBD"
                     /note="The ligand binding domain of nuclear receptors, a
                     family of ligand-activated transcription regulators;
                     cl11397"
                     /db_xref="CDD:472173"
     Site            order(107..108,111,114..115,144..145,148..149,152,155,189)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132726"
     Site            order(116,119,123,128,133..134,136..137,140..141)
                     /site_type="active"
                     /note="coregulator recognition site [active]"
                     /db_xref="CDD:132726"
     CDS             1..519
                     /gene="Eip75B"
                     /coded_by="XM_077646081.1:396..1955"
                     /db_xref="GeneID:144113155"
ORIGIN      
        1 msrdavrfgr vpkrekakil aamqkvnass qekalavele demrlfetvv raheetcdft
       61 sdkvspller arahpvytlc ppqlacplnp ttqpaegsga prlmedfser fspairgvve
      121 fakripgfsl laqddqvtll kagvfevllv rlacmfdsrs ssmvclngqv llreslhsas
      181 narflldsmf dfadrlnalg ltdaelglfc svvviapdrp glrntdlvqk mqrrlsevlq
      241 kavaaghpdr pqlcaellkk vpdlrtlntl hsekllaykm epatppyleq wgatedwspa
      301 sphsvasstn sldgakspvr sgsfssstdy spdspakpfa kvrrldsptd sgidsgkepg
      361 cctpsasvcs sprslddkaa depdradedm pvlkralqap plvsphqlme eayrhhkklr
      421 avrkeaasss adlasshstl vgrlqqaprf lneqqlkrtd lihniimrne gpqnffqgwg
      481 spprcpyspq pspplvpqdg pqplnlskkp ptpplktet
//
LOCUS       XP_077502208             519 aa            linear   INV 25-NOV-2025
DEFINITION  ecdysone-induced protein 75B isoform X5 [Amblyomma americanum].
ACCESSION   XP_077502208
VERSION     XP_077502208.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646082.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..519
                     /product="ecdysone-induced protein 75B isoform X5"
                     /calculated_mol_wt=56834
     Region          86..280
                     /region_name="NR_LBD"
                     /note="The ligand binding domain of nuclear receptors, a
                     family of ligand-activated transcription regulators;
                     cl11397"
                     /db_xref="CDD:472173"
     Site            order(107..108,111,114..115,144..145,148..149,152,155,189)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132726"
     Site            order(116,119,123,128,133..134,136..137,140..141)
                     /site_type="active"
                     /note="coregulator recognition site [active]"
                     /db_xref="CDD:132726"
     CDS             1..519
                     /gene="Eip75B"
                     /coded_by="XM_077646082.1:345..1904"
                     /db_xref="GeneID:144113155"
ORIGIN      
        1 mamravrfgr vpkrekakil aamqkvnass qekalavele demrlfetvv raheetcdft
       61 sdkvspller arahpvytlc ppqlacplnp ttqpaegsga prlmedfser fspairgvve
      121 fakripgfsl laqddqvtll kagvfevllv rlacmfdsrs ssmvclngqv llreslhsas
      181 narflldsmf dfadrlnalg ltdaelglfc svvviapdrp glrntdlvqk mqrrlsevlq
      241 kavaaghpdr pqlcaellkk vpdlrtlntl hsekllaykm epatppyleq wgatedwspa
      301 sphsvasstn sldgakspvr sgsfssstdy spdspakpfa kvrrldsptd sgidsgkepg
      361 cctpsasvcs sprslddkaa depdradedm pvlkralqap plvsphqlme eayrhhkklr
      421 avrkeaasss adlasshstl vgrlqqaprf lneqqlkrtd lihniimrne gpqnffqgwg
      481 spprcpyspq pspplvpqdg pqplnlskkp ptpplktet
//
LOCUS       XP_077502209             563 aa            linear   INV 25-NOV-2025
DEFINITION  monocarboxylate transporter 5-like [Amblyomma americanum].
ACCESSION   XP_077502209
VERSION     XP_077502209.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646083.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..563
                     /product="monocarboxylate transporter 5-like"
                     /calculated_mol_wt=61208
     Region          16..541
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(27..28,31..32,35,70,121..122,124..126,129,148,
                     151..152,155,372,375..376,379..381,384,411,415,466..467,
                     471,475,491,494..495,498..499,502)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:349949"
     CDS             1..563
                     /gene="LOC144113157"
                     /coded_by="XM_077646083.1:285..1976"
                     /db_xref="GeneID:144113157"
ORIGIN      
        1 mglsrkpalr arqlhglviv aavfwinmfv lgffraggvl yvalvrtyrc syeqaswpfs
       61 lagatlcmtg pfagalsrll sirlivsagv vvtavaissc fftdditvvv illgifygig
      121 tgmvcnltpl lltqcfenhr aiacgvaysg stigsffwpa lleylinefg lrgalliyga
      181 tilhgvmgsi lltplskaph reaeaeepkp eaeeekmlep lrekcqsldf hpfqnggnfa
      241 rryssrmsvr safaddsasa cavsvsglcm vlsdgdqwhs reehldqhha cspatslkkl
      301 ndpspleplw etgsapdtap dtrtsgkqss lkasltdvdr agsslcgnli rvvlndlkll
      361 rnyhfilvtt savgfffvfs tfiiiipdya tdkgltqseg vfllsvygit dlfskpipgl
      421 laykkiidnk gificggfit gltmlvmpsm dsywsfvlmt llfglvtggl ifmspvllte
      481 flgphlavma fgmsnmfigi asllrpfiig yfkdnwhsyd glfysmatac ilssliwfvg
      541 piaekvrtfv kapstspppp qev
//
LOCUS       XP_077502210             563 aa            linear   INV 25-NOV-2025
DEFINITION  monocarboxylate transporter 5-like [Amblyomma americanum].
ACCESSION   XP_077502210
VERSION     XP_077502210.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646084.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..563
                     /product="monocarboxylate transporter 5-like"
                     /calculated_mol_wt=61208
     Region          16..541
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(27..28,31..32,35,70,121..122,124..126,129,148,
                     151..152,155,372,375..376,379..381,384,411,415,466..467,
                     471,475,491,494..495,498..499,502)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:349949"
     CDS             1..563
                     /gene="LOC144113157"
                     /coded_by="XM_077646084.1:275..1966"
                     /db_xref="GeneID:144113157"
ORIGIN      
        1 mglsrkpalr arqlhglviv aavfwinmfv lgffraggvl yvalvrtyrc syeqaswpfs
       61 lagatlcmtg pfagalsrll sirlivsagv vvtavaissc fftdditvvv illgifygig
      121 tgmvcnltpl lltqcfenhr aiacgvaysg stigsffwpa lleylinefg lrgalliyga
      181 tilhgvmgsi lltplskaph reaeaeepkp eaeeekmlep lrekcqsldf hpfqnggnfa
      241 rryssrmsvr safaddsasa cavsvsglcm vlsdgdqwhs reehldqhha cspatslkkl
      301 ndpspleplw etgsapdtap dtrtsgkqss lkasltdvdr agsslcgnli rvvlndlkll
      361 rnyhfilvtt savgfffvfs tfiiiipdya tdkgltqseg vfllsvygit dlfskpipgl
      421 laykkiidnk gificggfit gltmlvmpsm dsywsfvlmt llfglvtggl ifmspvllte
      481 flgphlavma fgmsnmfigi asllrpfiig yfkdnwhsyd glfysmatac ilssliwfvg
      541 piaekvrtfv kapstspppp qev
//
LOCUS       XP_077502211             563 aa            linear   INV 25-NOV-2025
DEFINITION  monocarboxylate transporter 5-like [Amblyomma americanum].
ACCESSION   XP_077502211
VERSION     XP_077502211.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646085.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..563
                     /product="monocarboxylate transporter 5-like"
                     /calculated_mol_wt=61208
     Region          16..541
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(27..28,31..32,35,70,121..122,124..126,129,148,
                     151..152,155,372,375..376,379..381,384,411,415,466..467,
                     471,475,491,494..495,498..499,502)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:349949"
     CDS             1..563
                     /gene="LOC144113157"
                     /coded_by="XM_077646085.1:49..1740"
                     /db_xref="GeneID:144113157"
ORIGIN      
        1 mglsrkpalr arqlhglviv aavfwinmfv lgffraggvl yvalvrtyrc syeqaswpfs
       61 lagatlcmtg pfagalsrll sirlivsagv vvtavaissc fftdditvvv illgifygig
      121 tgmvcnltpl lltqcfenhr aiacgvaysg stigsffwpa lleylinefg lrgalliyga
      181 tilhgvmgsi lltplskaph reaeaeepkp eaeeekmlep lrekcqsldf hpfqnggnfa
      241 rryssrmsvr safaddsasa cavsvsglcm vlsdgdqwhs reehldqhha cspatslkkl
      301 ndpspleplw etgsapdtap dtrtsgkqss lkasltdvdr agsslcgnli rvvlndlkll
      361 rnyhfilvtt savgfffvfs tfiiiipdya tdkgltqseg vfllsvygit dlfskpipgl
      421 laykkiidnk gificggfit gltmlvmpsm dsywsfvlmt llfglvtggl ifmspvllte
      481 flgphlavma fgmsnmfigi asllrpfiig yfkdnwhsyd glfysmatac ilssliwfvg
      541 piaekvrtfv kapstspppp qev
//
LOCUS       XP_077502212             405 aa            linear   INV 25-NOV-2025
DEFINITION  endoplasmic reticulum protein 44 [Amblyomma americanum].
ACCESSION   XP_077502212
VERSION     XP_077502212.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646086.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..405
                     /product="endoplasmic reticulum protein 44"
                     /calculated_mol_wt=46332
     Region          31..137
                     /region_name="Thioredoxin_like"
                     /note="Protein Disulfide Oxidoreductases and Other
                     Proteins with a Thioredoxin fold; cl00388"
                     /db_xref="CDD:469754"
     Region          145..239
                     /region_name="PDI_b_family"
                     /note="Protein Disulfide Isomerase (PDIb) family, redox
                     inactive TRX-like domain b; composed of eukaryotic
                     proteins involved in oxidative protein folding in the
                     endoplasmic reticulum (ER) by acting as catalysts and
                     folding assistants. Members of this family...; cd02981"
                     /db_xref="CDD:239279"
     Region          248..358
                     /region_name="Thioredoxin_like"
                     /note="Protein Disulfide Oxidoreductases and Other
                     Proteins with a Thioredoxin fold; cl00388"
                     /db_xref="CDD:469754"
     CDS             1..405
                     /gene="ERp44"
                     /coded_by="XM_077646086.1:119..1336"
                     /db_xref="GeneID:144113158"
ORIGIN      
        1 mrcnvvsifn kligvvlflf lsrtkqasae svvaltssnf dehvgkyelv flnfyadwcr
       61 fsqilapvfe eaaknvhelt kepnkvlfgk vdcdretsia akhritkypt lklvrngmvm
      121 kreyrgqrsa eamknyilem lkdqvvevph eaeaqhveek kpallgyfag kdteayttfr
      181 rvasdlrddc nfyfvsrpag ntndttpdsi hfkpprskfg ekhetypslp hlfdelkkwa
      241 gekciplvre itfenaeelt eeglpflilf hhpddkesae lytkvvheql kndkhtinpl
      301 iadgvkfahp lqhlgktvkd lpliaidsfr hmyrfpdfsh lkipgklksf vddlysgklh
      361 refhygpdpt vggaetesqs tdppestfkn lapsrqryti lkdel
//
LOCUS       XP_077502213             454 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113159 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502213
VERSION     XP_077502213.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646087.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..454
                     /product="uncharacterized protein LOC144113159 isoform X1"
                     /calculated_mol_wt=50800
     Region          238..436
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(296,335,349,351)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..454
                     /gene="LOC144113159"
                     /coded_by="XM_077646087.1:589..1953"
                     /db_xref="GeneID:144113159"
ORIGIN      
        1 mkaykalesh nyftsgwvkh lsakefqdgk vvllgevnhs qrlghkplqv wilckkdgav
       61 itahctcmag ageacshvga clfavetgik mmksrsctqk dnmwlpayve kvqykrlkdi
      121 nftsskgkkr qldsnsfeag pvkerahvpp lseqertkly gsiqdagivp sifsvlpgyd
      181 ghfknpvpmh darlrnlyse emlahdldvl vkkandvmps iiiseetvka vealtrqqss
      241 ssnwflyrag rvtasvmkrv chtsidnpsi sllklicype kqslktpait wgvknepraf
      301 rayqtseaek hdmfkcsksg lhlstrypyv gatpdglvcc accgkgvvel kcpfslrevk
      361 dvtematags cleavngvvq lrrqhgyfyq vqtqmavcdv qwcdfvvwtp nllhveriqk
      421 drhfceeila agrkffihai lpeafqhllh qaac
//
LOCUS       XP_077502214             160 aa            linear   INV 25-NOV-2025
DEFINITION  natterin-3-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502214
VERSION     XP_077502214.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646088.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..160
                     /product="natterin-3-like isoform X2"
                     /calculated_mol_wt=17154
     Region          39..151
                     /region_name="DUF3421"
                     /note="Protein of unknown function (DUF3421); pfam11901"
                     /db_xref="CDD:463390"
     CDS             1..160
                     /gene="LOC144113159"
                     /coded_by="XM_077646088.1:271..753"
                     /db_xref="GeneID:144113159"
ORIGIN      
        1 mnpnwvwsap nalafmptim wvrsdvsnvp nnagpggkdg gektyigrae hngdlipgkv
       61 eashgccyvs yadgehryen yeilvfdgas vhwvassggs vpgraveggr tssgeklyig
      121 rthhegtvtv gkiqpshecl yipyggcehr ytgyealalq
//
LOCUS       XP_077502215             346 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113160 [Amblyomma americanum].
ACCESSION   XP_077502215
VERSION     XP_077502215.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646089.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..346
                     /product="uncharacterized protein LOC144113160"
                     /calculated_mol_wt=38627
     Region          73..124
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          155..323
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..346
                     /gene="LOC144113160"
                     /coded_by="XM_077646089.1:313..1353"
                     /db_xref="GeneID:144113160"
ORIGIN      
        1 mtmqdiqale qhsvvlnehl ytlqkekeql evtedsmksc eakvrlytgm pnfavlfavf
       61 ealasfishn vnnkltkfqe fvlfmmklkv nlqnadlafr fsiseatvsr ifdkwlhvay
      121 crlkdeiiwp trdalqktmp qaffdsfgan vaviidcfei kierpssylp rcetwsqykg
      181 sntakfligi apqgvvtyis egwggrasdk hitehcgfld nlvpgdvvla drgfnisesv
      241 gfycaklhvp aftrgkkqls aedvqstrkl anvrihvirv iglirnkfif lksvvpidyv
      301 vcrpgdevap ldkivtvccv lsnlcasiva apndatgqqa sasvde
//
LOCUS       XP_077502216             160 aa            linear   INV 25-NOV-2025
DEFINITION  natterin-3-like [Amblyomma americanum].
ACCESSION   XP_077502216
VERSION     XP_077502216.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646090.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..160
                     /product="natterin-3-like"
                     /calculated_mol_wt=17178
     Region          39..151
                     /region_name="DUF3421"
                     /note="Protein of unknown function (DUF3421); pfam11901"
                     /db_xref="CDD:463390"
     CDS             1..160
                     /gene="LOC144113162"
                     /coded_by="XM_077646090.1:105..587"
                     /db_xref="GeneID:144113162"
ORIGIN      
        1 mnpnwvwsap kalafmptim wvrsevgkvp nnavpggkdg gektyigrae hnadlipgkv
       61 eashgccyvs yadgehryen yeilvfdgas vhwvassgas vpgraveggr assgeklyig
      121 rthhegtvtm gkiqpshegl yipyggcehr ysgyealalq
//
LOCUS       XP_077502217             158 aa            linear   INV 25-NOV-2025
DEFINITION  natterin-3-like [Amblyomma americanum].
ACCESSION   XP_077502217
VERSION     XP_077502217.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646091.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="natterin-3-like"
                     /calculated_mol_wt=17026
     Region          33..145
                     /region_name="DUF3421"
                     /note="Protein of unknown function (DUF3421); pfam11901"
                     /db_xref="CDD:463390"
     CDS             1..158
                     /gene="LOC144113163"
                     /coded_by="XM_077646091.1:142..618"
                     /db_xref="GeneID:144113163"
ORIGIN      
        1 megygaqvyr pvcewvmcfe nripfnavpg gedsgetiyi gravhngevv pgkvvpshsc
       61 cyvaydgveh shkeyqalis egtpfawvpa sdgvlptgav qggvcasgep lyigrtyheg
      121 tltigkiqps rrclaipygg eehcypdyev lvvqtvnf
//
LOCUS       XP_077502218             125 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113165 [Amblyomma americanum].
ACCESSION   XP_077502218
VERSION     XP_077502218.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646092.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..125
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..125
                     /product="uncharacterized protein LOC144113165"
                     /calculated_mol_wt=13114
     CDS             1..125
                     /gene="LOC144113165"
                     /coded_by="XM_077646092.1:26..403"
                     /db_xref="GeneID:144113165"
ORIGIN      
        1 miedaavgir lltqenaise lrgqevrvrw qegqdaapae llafgkptvl erkrkkivaa
       61 aasaeeiata sstsvqgsga aspvqqlccs aalempella kiaeltaene vlkrklesae
      121 ttvgk
//
LOCUS       XP_077502219              77 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113166 [Amblyomma americanum].
ACCESSION   XP_077502219
VERSION     XP_077502219.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646093.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..77
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..77
                     /product="uncharacterized protein LOC144113166"
                     /calculated_mol_wt=8209
     Region          6..70
                     /region_name="DM9"
                     /note="Repeats found in Drosophila proteins; smart00696"
                     /db_xref="CDD:128937"
     CDS             1..77
                     /gene="LOC144113166"
                     /coded_by="XM_077646093.1:25..258"
                     /db_xref="GeneID:144113166"
ORIGIN      
        1 mptimwvrsd vsnvpnnagp ggkdggekty igraehngdl ipgkveashg ccyvsyadge
       61 hryenyeilv fdgasvh
//
LOCUS       XP_077502220             334 aa            linear   INV 25-NOV-2025
DEFINITION  mortality factor 4-like protein 1 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502220
VERSION     XP_077502220.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646094.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..334
                     /product="mortality factor 4-like protein 1 isoform X1"
                     /calculated_mol_wt=38325
     Region          11..67
                     /region_name="CBD_MSL3_like"
                     /note="chromo barrel domain of human male-specific lethal
                     complex subunit 3, and similar proteins; cd18983"
                     /db_xref="CDD:350846"
     Site            order(17..18,22..25,40,42..45,49,51..54,56..58,61..62)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:350846"
     Site            order(22,42,45,49)
                     /site_type="other"
                     /note="putative methylated histone tail binding site
                     [chemical binding]"
                     /db_xref="CDD:350846"
     Site            order(39,41..46,48..52)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:350846"
     Site            order(39,42,46,48,52)
                     /site_type="other"
                     /note="putative RNA binding site [nucleotide binding]"
                     /db_xref="CDD:350846"
     Region          148..322
                     /region_name="MRG"
                     /note="pfam05712"
                     /db_xref="CDD:461721"
     CDS             1..334
                     /gene="LOC144113167"
                     /coded_by="XM_077646094.1:117..1121"
                     /db_xref="GeneID:144113167"
ORIGIN      
        1 mapktrfqdg ekvlcfhgpl lyeakcikaq vkdkqtkyfi hysgwnknwd ewvpesrvlk
       61 fndvnlqkqk elekahlsaa shshsalkgk knkatkpkke vdkersstps qerpqkqkgt
      121 aaassvtshq pstsqatess geshrkkrsr qdphiepeet fvirtevkvr ipdelkpwlv
      181 ddwefvnqqk klvqlpcnlt vdqifadyvk qktsvkgiss nkvsailevt nglkeyfnvm
      241 lgsqllykfe rtqyteilnd rpdtpmsqiy gashllrlfv kigamlsytp fdekciqlll
      301 tqtheflkym akntqlfnln dyavaspdyq rkai
//
LOCUS       XP_077502221             324 aa            linear   INV 25-NOV-2025
DEFINITION  mortality factor 4-like protein 1 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502221
VERSION     XP_077502221.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646095.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..324
                     /product="mortality factor 4-like protein 1 isoform X2"
                     /calculated_mol_wt=37376
     Region          11..67
                     /region_name="CBD_MSL3_like"
                     /note="chromo barrel domain of human male-specific lethal
                     complex subunit 3, and similar proteins; cd18983"
                     /db_xref="CDD:350846"
     Site            order(17..18,22..25,40,42..45,49,51..54,56..58,61..62)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:350846"
     Site            order(22,42,45,49)
                     /site_type="other"
                     /note="putative methylated histone tail binding site
                     [chemical binding]"
                     /db_xref="CDD:350846"
     Site            order(39,41..46,48..52)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:350846"
     Site            order(39,42,46,48,52)
                     /site_type="other"
                     /note="putative RNA binding site [nucleotide binding]"
                     /db_xref="CDD:350846"
     Region          138..312
                     /region_name="MRG"
                     /note="pfam05712"
                     /db_xref="CDD:461721"
     CDS             1..324
                     /gene="LOC144113167"
                     /coded_by="XM_077646095.1:116..1090"
                     /db_xref="GeneID:144113167"
ORIGIN      
        1 mapktrfqdg ekvlcfhgpl lyeakcikaq vkdkqtkyfi hysgwnknwd ewvpesrvlk
       61 fndvnlqkqk elekahlkgk knkatkpkke vdkersstps qerpqkqkgt aaassvtshq
      121 pstsqatess geshrkkrsr qdphiepeet fvirtevkvr ipdelkpwlv ddwefvnqqk
      181 klvqlpcnlt vdqifadyvk qktsvkgiss nkvsailevt nglkeyfnvm lgsqllykfe
      241 rtqyteilnd rpdtpmsqiy gashllrlfv kigamlsytp fdekciqlll tqtheflkym
      301 akntqlfnln dyavaspdyq rkai
//
LOCUS       XP_077502222             207 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113168 [Amblyomma americanum].
ACCESSION   XP_077502222
VERSION     XP_077502222.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646096.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..207
                     /product="uncharacterized protein LOC144113168"
                     /calculated_mol_wt=23680
     Region          13..100
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..207
                     /gene="LOC144113168"
                     /coded_by="XM_077646096.1:69..692"
                     /db_xref="GeneID:144113168"
ORIGIN      
        1 maaavgprav straswtdre vecflalise kkvsealdsr rqrnqdvfkd lqaemanlgy
       61 hwtwqqlrnc wndlkkrfta erleqersga apsawkwydh msallshrpk vqareygvds
      121 qdvppdygdn sqldisdadt rteeddadil qeqsepstss rsppqkrrrm geaklekvll
      181 nvqrkkrril deasrdtisa tkttacr
//
LOCUS       XP_077502223             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113170 [Amblyomma americanum].
ACCESSION   XP_077502223
VERSION     XP_077502223.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646097.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144113170"
                     /calculated_mol_wt=23743
     CDS             1..217
                     /gene="LOC144113170"
                     /coded_by="XM_077646097.1:262..915"
                     /db_xref="GeneID:144113170"
ORIGIN      
        1 mqpvqvlrla rllnappaqr hsasgariqr cappvqrlsa tgttiarslp lknrsgsaer
       61 tqesppqwkl gpaaertegs ppqkqklsta aekswpfvrp rpkcraikvl lvsllaapmn
      121 vvtpgvcpal rsvpvpisas fdwdvddslv flfhhfpafp hwhpglaaav nliwqqgptg
      181 gilsegrilk rplqdvrgta fwsrdytfst prvhqqn
//
LOCUS       XP_077502224             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113170 [Amblyomma americanum].
ACCESSION   XP_077502224
VERSION     XP_077502224.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646098.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144113170"
                     /calculated_mol_wt=23743
     CDS             1..217
                     /gene="LOC144113170"
                     /coded_by="XM_077646098.1:262..915"
                     /db_xref="GeneID:144113170"
ORIGIN      
        1 mqpvqvlrla rllnappaqr hsasgariqr cappvqrlsa tgttiarslp lknrsgsaer
       61 tqesppqwkl gpaaertegs ppqkqklsta aekswpfvrp rpkcraikvl lvsllaapmn
      121 vvtpgvcpal rsvpvpisas fdwdvddslv flfhhfpafp hwhpglaaav nliwqqgptg
      181 gilsegrilk rplqdvrgta fwsrdytfst prvhqqn
//
LOCUS       XP_077502225             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113170 [Amblyomma americanum].
ACCESSION   XP_077502225
VERSION     XP_077502225.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646099.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144113170"
                     /calculated_mol_wt=23743
     CDS             1..217
                     /gene="LOC144113170"
                     /coded_by="XM_077646099.1:262..915"
                     /db_xref="GeneID:144113170"
ORIGIN      
        1 mqpvqvlrla rllnappaqr hsasgariqr cappvqrlsa tgttiarslp lknrsgsaer
       61 tqesppqwkl gpaaertegs ppqkqklsta aekswpfvrp rpkcraikvl lvsllaapmn
      121 vvtpgvcpal rsvpvpisas fdwdvddslv flfhhfpafp hwhpglaaav nliwqqgptg
      181 gilsegrilk rplqdvrgta fwsrdytfst prvhqqn
//
LOCUS       XP_077502226             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113170 [Amblyomma americanum].
ACCESSION   XP_077502226
VERSION     XP_077502226.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646100.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144113170"
                     /calculated_mol_wt=23743
     CDS             1..217
                     /gene="LOC144113170"
                     /coded_by="XM_077646100.1:262..915"
                     /db_xref="GeneID:144113170"
ORIGIN      
        1 mqpvqvlrla rllnappaqr hsasgariqr cappvqrlsa tgttiarslp lknrsgsaer
       61 tqesppqwkl gpaaertegs ppqkqklsta aekswpfvrp rpkcraikvl lvsllaapmn
      121 vvtpgvcpal rsvpvpisas fdwdvddslv flfhhfpafp hwhpglaaav nliwqqgptg
      181 gilsegrilk rplqdvrgta fwsrdytfst prvhqqn
//
LOCUS       XP_077502227             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113170 [Amblyomma americanum].
ACCESSION   XP_077502227
VERSION     XP_077502227.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646101.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144113170"
                     /calculated_mol_wt=23743
     CDS             1..217
                     /gene="LOC144113170"
                     /coded_by="XM_077646101.1:218..871"
                     /db_xref="GeneID:144113170"
ORIGIN      
        1 mqpvqvlrla rllnappaqr hsasgariqr cappvqrlsa tgttiarslp lknrsgsaer
       61 tqesppqwkl gpaaertegs ppqkqklsta aekswpfvrp rpkcraikvl lvsllaapmn
      121 vvtpgvcpal rsvpvpisas fdwdvddslv flfhhfpafp hwhpglaaav nliwqqgptg
      181 gilsegrilk rplqdvrgta fwsrdytfst prvhqqn
//
LOCUS       XP_077502228             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113170 [Amblyomma americanum].
ACCESSION   XP_077502228
VERSION     XP_077502228.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646102.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144113170"
                     /calculated_mol_wt=23743
     CDS             1..217
                     /gene="LOC144113170"
                     /coded_by="XM_077646102.1:464..1117"
                     /db_xref="GeneID:144113170"
ORIGIN      
        1 mqpvqvlrla rllnappaqr hsasgariqr cappvqrlsa tgttiarslp lknrsgsaer
       61 tqesppqwkl gpaaertegs ppqkqklsta aekswpfvrp rpkcraikvl lvsllaapmn
      121 vvtpgvcpal rsvpvpisas fdwdvddslv flfhhfpafp hwhpglaaav nliwqqgptg
      181 gilsegrilk rplqdvrgta fwsrdytfst prvhqqn
//
LOCUS       XP_077502229             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113171 [Amblyomma americanum].
ACCESSION   XP_077502229
VERSION     XP_077502229.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646103.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144113171"
                     /calculated_mol_wt=23772
     CDS             1..217
                     /gene="LOC144113171"
                     /coded_by="XM_077646103.1:254..907"
                     /db_xref="GeneID:144113171"
ORIGIN      
        1 mqpvqvlrla rllnappaqr hsasgariqr cappvqrlsa tgttiarslp lknrsgsaer
       61 tresppqwkl gpaaertegs ppqkqklsta aekswpfvrp rpkcraikvl lvsllaapmn
      121 vvtpgvcpal rsvpvpisas fdwdvddslv flfhhfpafp hwhpglaaav nliwqqgptg
      181 gilsegrilk rplqdvrgta fwsrdytfst prvhqqn
//
LOCUS       XP_077502230             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113171 [Amblyomma americanum].
ACCESSION   XP_077502230
VERSION     XP_077502230.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646104.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144113171"
                     /calculated_mol_wt=23772
     CDS             1..217
                     /gene="LOC144113171"
                     /coded_by="XM_077646104.1:254..907"
                     /db_xref="GeneID:144113171"
ORIGIN      
        1 mqpvqvlrla rllnappaqr hsasgariqr cappvqrlsa tgttiarslp lknrsgsaer
       61 tresppqwkl gpaaertegs ppqkqklsta aekswpfvrp rpkcraikvl lvsllaapmn
      121 vvtpgvcpal rsvpvpisas fdwdvddslv flfhhfpafp hwhpglaaav nliwqqgptg
      181 gilsegrilk rplqdvrgta fwsrdytfst prvhqqn
//
LOCUS       XP_077502231             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113171 [Amblyomma americanum].
ACCESSION   XP_077502231
VERSION     XP_077502231.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646105.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144113171"
                     /calculated_mol_wt=23772
     CDS             1..217
                     /gene="LOC144113171"
                     /coded_by="XM_077646105.1:254..907"
                     /db_xref="GeneID:144113171"
ORIGIN      
        1 mqpvqvlrla rllnappaqr hsasgariqr cappvqrlsa tgttiarslp lknrsgsaer
       61 tresppqwkl gpaaertegs ppqkqklsta aekswpfvrp rpkcraikvl lvsllaapmn
      121 vvtpgvcpal rsvpvpisas fdwdvddslv flfhhfpafp hwhpglaaav nliwqqgptg
      181 gilsegrilk rplqdvrgta fwsrdytfst prvhqqn
//
LOCUS       XP_077502232             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113171 [Amblyomma americanum].
ACCESSION   XP_077502232
VERSION     XP_077502232.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646106.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144113171"
                     /calculated_mol_wt=23772
     CDS             1..217
                     /gene="LOC144113171"
                     /coded_by="XM_077646106.1:254..907"
                     /db_xref="GeneID:144113171"
ORIGIN      
        1 mqpvqvlrla rllnappaqr hsasgariqr cappvqrlsa tgttiarslp lknrsgsaer
       61 tresppqwkl gpaaertegs ppqkqklsta aekswpfvrp rpkcraikvl lvsllaapmn
      121 vvtpgvcpal rsvpvpisas fdwdvddslv flfhhfpafp hwhpglaaav nliwqqgptg
      181 gilsegrilk rplqdvrgta fwsrdytfst prvhqqn
//
LOCUS       XP_077502233             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113171 [Amblyomma americanum].
ACCESSION   XP_077502233
VERSION     XP_077502233.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646107.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144113171"
                     /calculated_mol_wt=23772
     CDS             1..217
                     /gene="LOC144113171"
                     /coded_by="XM_077646107.1:254..907"
                     /db_xref="GeneID:144113171"
ORIGIN      
        1 mqpvqvlrla rllnappaqr hsasgariqr cappvqrlsa tgttiarslp lknrsgsaer
       61 tresppqwkl gpaaertegs ppqkqklsta aekswpfvrp rpkcraikvl lvsllaapmn
      121 vvtpgvcpal rsvpvpisas fdwdvddslv flfhhfpafp hwhpglaaav nliwqqgptg
      181 gilsegrilk rplqdvrgta fwsrdytfst prvhqqn
//
LOCUS       XP_077502234             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113171 [Amblyomma americanum].
ACCESSION   XP_077502234
VERSION     XP_077502234.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646108.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144113171"
                     /calculated_mol_wt=23772
     CDS             1..217
                     /gene="LOC144113171"
                     /coded_by="XM_077646108.1:254..907"
                     /db_xref="GeneID:144113171"
ORIGIN      
        1 mqpvqvlrla rllnappaqr hsasgariqr cappvqrlsa tgttiarslp lknrsgsaer
       61 tresppqwkl gpaaertegs ppqkqklsta aekswpfvrp rpkcraikvl lvsllaapmn
      121 vvtpgvcpal rsvpvpisas fdwdvddslv flfhhfpafp hwhpglaaav nliwqqgptg
      181 gilsegrilk rplqdvrgta fwsrdytfst prvhqqn
//
LOCUS       XP_077502235             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113171 [Amblyomma americanum].
ACCESSION   XP_077502235
VERSION     XP_077502235.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646109.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144113171"
                     /calculated_mol_wt=23772
     CDS             1..217
                     /gene="LOC144113171"
                     /coded_by="XM_077646109.1:254..907"
                     /db_xref="GeneID:144113171"
ORIGIN      
        1 mqpvqvlrla rllnappaqr hsasgariqr cappvqrlsa tgttiarslp lknrsgsaer
       61 tresppqwkl gpaaertegs ppqkqklsta aekswpfvrp rpkcraikvl lvsllaapmn
      121 vvtpgvcpal rsvpvpisas fdwdvddslv flfhhfpafp hwhpglaaav nliwqqgptg
      181 gilsegrilk rplqdvrgta fwsrdytfst prvhqqn
//
LOCUS       XP_077502236             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113171 [Amblyomma americanum].
ACCESSION   XP_077502236
VERSION     XP_077502236.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646110.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144113171"
                     /calculated_mol_wt=23772
     CDS             1..217
                     /gene="LOC144113171"
                     /coded_by="XM_077646110.1:204..857"
                     /db_xref="GeneID:144113171"
ORIGIN      
        1 mqpvqvlrla rllnappaqr hsasgariqr cappvqrlsa tgttiarslp lknrsgsaer
       61 tresppqwkl gpaaertegs ppqkqklsta aekswpfvrp rpkcraikvl lvsllaapmn
      121 vvtpgvcpal rsvpvpisas fdwdvddslv flfhhfpafp hwhpglaaav nliwqqgptg
      181 gilsegrilk rplqdvrgta fwsrdytfst prvhqqn
//
LOCUS       XP_077502237             147 aa            linear   INV 25-NOV-2025
DEFINITION  natterin-4-like [Amblyomma americanum].
ACCESSION   XP_077502237
VERSION     XP_077502237.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646111.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..147
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..147
                     /product="natterin-4-like"
                     /calculated_mol_wt=16107
     Region          25..135
                     /region_name="DUF3421"
                     /note="Protein of unknown function (DUF3421); pfam11901"
                     /db_xref="CDD:463390"
     CDS             1..147
                     /gene="LOC144113172"
                     /coded_by="XM_077646111.1:99..542"
                     /db_xref="GeneID:144113172"
ORIGIN      
        1 msvrwaccya dniphnavvg gnakgedlyv grawhnggll pgkvvrslqc cyvpcnnseh
       61 kyqhyeilvn dgaelkwips cdghvpdeai pggksaaget lyigrtrheg tvtcgkvhps
      121 qgllyipyyh rehryngyev lvnagar
//
LOCUS       XP_077502238             159 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113173 [Amblyomma americanum].
ACCESSION   XP_077502238
VERSION     XP_077502238.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646112.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..159
                     /product="uncharacterized protein LOC144113173"
                     /calculated_mol_wt=17893
     Region          <11..44
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..159
                     /gene="LOC144113173"
                     /coded_by="XM_077646112.1:149..628"
                     /db_xref="GeneID:144113173"
ORIGIN      
        1 mwekltdqin sefgchltpl qvenkwktme raykkskakn gssghsraac lyerelsevl
       61 erehhitpva llapgrivan reeenmlpnn gpadeedepp apsrsteatp arrrsrqdkt
      121 aigglikvlq eamaekkqqh eekmsllerl vkaveaknk
//
LOCUS       XP_077502239             146 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113174 [Amblyomma americanum].
ACCESSION   XP_077502239
VERSION     XP_077502239.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646113.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..146
                     /product="uncharacterized protein LOC144113174"
                     /calculated_mol_wt=15819
     CDS             1..146
                     /gene="LOC144113174"
                     /coded_by="XM_077646113.1:951..1391"
                     /db_xref="GeneID:144113174"
ORIGIN      
        1 mavviglpav pnsfpketsv aplqertarc pmssvdplsl psgsvqcrli speilastlr
       61 pvpmwlllfp sladnsfytw qhgsstisdi gdayknkpgt thlghdtvia meilplqilq
      121 klaprmlfsk enpelvdhlf lcakgk
//
LOCUS       XP_077502240             232 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113175 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502240
VERSION     XP_077502240.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646114.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..232
                     /product="uncharacterized protein LOC144113175 isoform X1"
                     /calculated_mol_wt=25091
     CDS             1..232
                     /gene="LOC144113175"
                     /coded_by="XM_077646114.1:598..1296"
                     /db_xref="GeneID:144113175"
ORIGIN      
        1 mwppmhdlle pcapllnmvl trlssllssp sislglsfds lrggkttved qrcnvkrhgl
       61 rkqailecat agdvkmmhel gglghfcgle dhilqlgvlp lvatssagdr vvagvfaacv
      121 afdscppaas aaqkiltgsh amatvhkrdy vvnlvnwfrl vddvvlhcnl rlqtllegwe
      181 rlrvfslpql ktliasapdt gidnlfiape alralvsscp qllqhcrlpl mq
//
LOCUS       XP_077502241             232 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113175 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502241
VERSION     XP_077502241.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646115.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..232
                     /product="uncharacterized protein LOC144113175 isoform X1"
                     /calculated_mol_wt=25091
     CDS             1..232
                     /gene="LOC144113175"
                     /coded_by="XM_077646115.1:598..1296"
                     /db_xref="GeneID:144113175"
ORIGIN      
        1 mwppmhdlle pcapllnmvl trlssllssp sislglsfds lrggkttved qrcnvkrhgl
       61 rkqailecat agdvkmmhel gglghfcgle dhilqlgvlp lvatssagdr vvagvfaacv
      121 afdscppaas aaqkiltgsh amatvhkrdy vvnlvnwfrl vddvvlhcnl rlqtllegwe
      181 rlrvfslpql ktliasapdt gidnlfiape alralvsscp qllqhcrlpl mq
//
LOCUS       XP_077502242             232 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113175 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502242
VERSION     XP_077502242.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646116.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..232
                     /product="uncharacterized protein LOC144113175 isoform X1"
                     /calculated_mol_wt=25091
     CDS             1..232
                     /gene="LOC144113175"
                     /coded_by="XM_077646116.1:598..1296"
                     /db_xref="GeneID:144113175"
ORIGIN      
        1 mwppmhdlle pcapllnmvl trlssllssp sislglsfds lrggkttved qrcnvkrhgl
       61 rkqailecat agdvkmmhel gglghfcgle dhilqlgvlp lvatssagdr vvagvfaacv
      121 afdscppaas aaqkiltgsh amatvhkrdy vvnlvnwfrl vddvvlhcnl rlqtllegwe
      181 rlrvfslpql ktliasapdt gidnlfiape alralvsscp qllqhcrlpl mq
//
LOCUS       XP_077502243             221 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113175 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502243
VERSION     XP_077502243.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646117.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..221
                     /product="uncharacterized protein LOC144113175 isoform X2"
                     /calculated_mol_wt=23757
     CDS             1..221
                     /gene="LOC144113175"
                     /coded_by="XM_077646117.1:598..1263"
                     /db_xref="GeneID:144113175"
ORIGIN      
        1 mwppmhdlle pcapllnmvl trlssllssp sislglsfds lrggkttved qrcnvkrhgl
       61 rkqailecat agdvkmmhel gglghfcgle dhilqlgvlp lvatssagdr vvagvfaacv
      121 afdscppaas aaqkiltgsh amatvhkrdy vvnlvnwfrl vddvvlhcnl rlqtllegwe
      181 rlrvfslpql ktliasapdt gidnlfiape alralvsscp q
//
LOCUS       XP_077502244             202 aa            linear   INV 25-NOV-2025
DEFINITION  protein regulator of cytokinesis 1-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502244
VERSION     XP_077502244.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646118.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..202
                     /product="protein regulator of cytokinesis 1-like isoform
                     X3"
                     /calculated_mol_wt=23457
     Region          <90..197
                     /region_name="MAP65_ASE1"
                     /note="Microtubule associated protein (MAP65/ASE1 family);
                     pfam03999"
                     /db_xref="CDD:427641"
     CDS             1..202
                     /gene="LOC144113175"
                     /coded_by="XM_077646118.1:598..1206"
                     /db_xref="GeneID:144113175"
ORIGIN      
        1 mwppmhdlle pcapllnmvl trlssllssp sislglsfds lrggkttved qrcnvkrhgl
       61 rkqailecat agdfsdrrle eleeelrrlk efhaknkpil mkverrealw arfvefekra
      121 adpfclnncg gslllemrer krleaelprl eeeikthisg htgvedeqff tewaedflkh
      181 lssqheafnl ekdrerqert sr
//
LOCUS       XP_077502245             202 aa            linear   INV 25-NOV-2025
DEFINITION  protein regulator of cytokinesis 1-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502245
VERSION     XP_077502245.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646119.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..202
                     /product="protein regulator of cytokinesis 1-like isoform
                     X3"
                     /calculated_mol_wt=23457
     Region          <90..197
                     /region_name="MAP65_ASE1"
                     /note="Microtubule associated protein (MAP65/ASE1 family);
                     pfam03999"
                     /db_xref="CDD:427641"
     CDS             1..202
                     /gene="LOC144113175"
                     /coded_by="XM_077646119.1:598..1206"
                     /db_xref="GeneID:144113175"
ORIGIN      
        1 mwppmhdlle pcapllnmvl trlssllssp sislglsfds lrggkttved qrcnvkrhgl
       61 rkqailecat agdfsdrrle eleeelrrlk efhaknkpil mkverrealw arfvefekra
      121 adpfclnncg gslllemrer krleaelprl eeeikthisg htgvedeqff tewaedflkh
      181 lssqheafnl ekdrerqert sr
//
LOCUS       XP_077502246             277 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113178 [Amblyomma americanum].
ACCESSION   XP_077502246
VERSION     XP_077502246.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646120.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..277
                     /product="uncharacterized protein LOC144113178"
                     /calculated_mol_wt=30167
     CDS             1..277
                     /gene="LOC144113178"
                     /coded_by="XM_077646120.1:303..1136"
                     /db_xref="GeneID:144113178"
ORIGIN      
        1 mtghmllyer llkklpkdte knircngdgh yvvpsgsspd sklytvysdi glctcrsgqq
       61 safckhqalv hkvfggifpn aplltteary nlgklllgdn cpgidffegl hetqvlqnaa
      121 cskaptdald cwdtpststa pttqysppes retstaqlmn qeleedpfea lieafqhlka
      181 evgndptcmk htarlakklk slpskqsfvt aminmsaavc rvsrcrgkis vqptsisrrr
      241 pglnkgskcv ppgrpskkak rspslcrsve dnipaak
//
LOCUS       XP_077502247             277 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113180 [Amblyomma americanum].
ACCESSION   XP_077502247
VERSION     XP_077502247.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646121.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..277
                     /product="uncharacterized protein LOC144113180"
                     /calculated_mol_wt=30162
     CDS             1..277
                     /gene="LOC144113180"
                     /coded_by="XM_077646121.1:65..898"
                     /db_xref="GeneID:144113180"
ORIGIN      
        1 mtghmllyer llkklpkdte knircngdgh yvvpsgsspd sklytvysdi glctcrsgqq
       61 gafckhqalv hkvfggifpn aplltteary nlgklllgdn cpgidffegl hetqvlqnaa
      121 cskaptdald cwdtpststa pttqysppes retstaqlmn qeleedpfea liqafqhlka
      181 evgndptcmk htarlakklk slpskqsfvt aminmsaavc rvsrcrgkis vqptsisrrr
      241 pglnkgskcv ppgrpskkak rshslcrsve dnvpaak
//
LOCUS       XP_077502248             612 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113181 [Amblyomma americanum].
ACCESSION   XP_077502248
VERSION     XP_077502248.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646122.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..612
                     /product="uncharacterized protein LOC144113181"
                     /calculated_mol_wt=67409
     CDS             1..612
                     /gene="LOC144113181"
                     /coded_by="XM_077646122.1:769..2607"
                     /db_xref="GeneID:144113181"
ORIGIN      
        1 mtspkkrdfr drkystkhkf rgrrkakrrt tsagsvpdpr parpiddsps deigaafsfv
       61 sasekkiaff ekdkrtsdhg sanvvmcdig altalvngap cptcgenqla vrepvnkrkg
      121 lasflelhce nvacpvtvls stytsrrvtr sgdasvkqgf dggssrdgfa snvkavvaar
      181 aigvgyqqlv rfcvimglpk pmhhktfdai tkkvhaaavs avvknlekar eatknevggl
      241 daavmfdgtw qkrghkshng vgtvvsletg lcldfevlsn ychgcsrhke lpgeeeiwqa
      301 fhgpvceknv dcsshameae aarriwqrtl syetplrftk flsdgdskay vavcdaqvyg
      361 plniekedct nhvakrlgsa lrqlktplpr gqklkesavt klqtyyqiav tsnqgsiqkm
      421 ysaiwasyfh scssdgassh kfcpggpesw ckhrraealc qpapnhtpll tkaqgkavlp
      481 iykrltdekl larciqgktq naaeslnsri wllcpktkfa srtvvemata lavlwfnegh
      541 asfervleel gvlpshqllm ltdsqnerri krmsacqtae arahrrsmak rarvedssrk
      601 dregvtysag qf
//
LOCUS       XP_077502249             487 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113182 [Amblyomma americanum].
ACCESSION   XP_077502249
VERSION     XP_077502249.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646123.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..487
                     /product="uncharacterized protein LOC144113182"
                     /calculated_mol_wt=54203
     Region          <9..80
                     /region_name="MULE"
                     /note="MULE transposase domain; pfam10551"
                     /db_xref="CDD:463145"
     Region          <42..180
                     /region_name="Transposase_mut"
                     /note="Transposase, Mutator family; cl27632"
                     /db_xref="CDD:474953"
     CDS             1..487
                     /gene="LOC144113182"
                     /coded_by="XM_077646123.1:1128..2591"
                     /db_xref="GeneID:144113182"
ORIGIN      
        1 mltatsagai piavivhssr ttegysagfe llkhnypfcf ggceapetfm tdnsaaekaa
       61 ilehwpearq qlchfhvaqa ewlwltlgkh gvspserksl maifqkimya nskdtleaak
      121 qdlysqqhkg fiphvdslle reeewvllfr lsaktrghnt nnysevlirv lkdiilhrtk
      181 afnvatlaef lsvvwekyff hrildhahsr mtghmllyer llkklpkdte knircngdgh
      241 yvvpsgsspd sklytvysdi slctcrsgqq gafckhqalv hkvfggifpn aplltteary
      301 nlgklllgdn fpgidffegl hetqvlqnaa cskaptdald cwdtpststa pttqysppes
      361 retstaqlmn qeleedpfea lieafqhlka evgndpacmk htarmakklk slpskqsfvt
      421 aminmsaavc rvsrcrgkis vqptsisrrr pglnkgskcv ppgrpskkak rshslcrsve
      481 dnvpaak
//
LOCUS       XP_077502250             427 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113183 [Amblyomma americanum].
ACCESSION   XP_077502250
VERSION     XP_077502250.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646124.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..427
                     /product="uncharacterized protein LOC144113183"
                     /calculated_mol_wt=47228
     CDS             1..427
                     /gene="LOC144113183"
                     /coded_by="XM_077646124.1:138..1421"
                     /db_xref="GeneID:144113183"
ORIGIN      
        1 mgyeqlvrfs avlglpkpmh hksfsaitrk vrdaamsavs enlarsrqit hseaggdeia
       61 vmydgtwhkr ghkshngigt vvsldtglcl dfevlsnfcl acsrhkalpn ediwqafhgp
      121 vcernvdcss hameaeaavr iwrrsqsydt plqfkkflsd gdskayaavv eanvyggaie
      181 kedctnhvak rlgtalrklk eplprgeklk epaiqrltty yqvaitsnrg svkgmhqaiw
      241 asyfhscssd gasshkycpd gpaswckhrr aealgeaapa htplltkaqg kailpiykrl
      301 tdeklltrcv qgktqnaaes lnskiwllcp ktrfasrfvv ematalavlw fnrghssfer
      361 vleelgvlpp qdlvglgssr dsmrqkkmsa kqtaesrarr cmlrnksrad dsackrrege
      421 tygagsf
//
LOCUS       XP_077502251             255 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113185 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502251
VERSION     XP_077502251.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646125.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..255
                     /product="uncharacterized protein LOC144113185 isoform X1"
                     /calculated_mol_wt=29354
     Region          <139..237
                     /region_name="MAP65_ASE1"
                     /note="Microtubule associated protein (MAP65/ASE1 family);
                     pfam03999"
                     /db_xref="CDD:427641"
     CDS             1..255
                     /gene="LOC144113185"
                     /coded_by="XM_077646125.1:1298..2065"
                     /db_xref="GeneID:144113185"
ORIGIN      
        1 mmhelggldn fggleddili sspsrgngas qlssslhmpr glhledhgqy atttaesfvs
       61 rscrlplrhs aecrismhga egevhllpss dtansirttv rfiisrlnci snslpllyqr
      121 ieeleeelrr lkefhakskp ilmkverrea lsarfvefek raadpfrlnn cgghlllemr
      181 erkrleaelp rleeeiktrr htgvedkqff tewaedflkh lssqheaynl ekyrerqert
      241 fqigglknwr ksseg
//
LOCUS       XP_077502253             219 aa            linear   INV 25-NOV-2025
DEFINITION  protein regulator of cytokinesis 1-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502253
VERSION     XP_077502253.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646127.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..219
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..219
                     /product="protein regulator of cytokinesis 1-like isoform
                     X2"
                     /calculated_mol_wt=25386
     Region          <103..201
                     /region_name="MAP65_ASE1"
                     /note="Microtubule associated protein (MAP65/ASE1 family);
                     pfam03999"
                     /db_xref="CDD:427641"
     CDS             1..219
                     /gene="LOC144113185"
                     /coded_by="XM_077646127.1:1298..1957"
                     /db_xref="GeneID:144113185"
ORIGIN      
        1 mmhelggldn fggleddili sspsrgngas qlssslhmpr glhledhgqy atttaesfvs
       61 rscrlplrhs aecrismhga edqrieelee elrrlkefha kskpilmkve rrealsarfv
      121 efekraadpf rlnncgghll lemrerkrle aelprleeei ktrrhtgved kqfftewaed
      181 flkhlssqhe aynlekyrer qertfqiggl knwrksseg
//
LOCUS       XP_077502254             255 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113186 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502254
VERSION     XP_077502254.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646128.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..255
                     /product="uncharacterized protein LOC144113186 isoform X1"
                     /calculated_mol_wt=28018
     CDS             1..255
                     /gene="LOC144113186"
                     /coded_by="XM_077646128.1:27..794"
                     /db_xref="GeneID:144113186"
ORIGIN      
        1 mvtlqrahvk hvflpwcflq lremwppmhd llepcaplln mvltrlssll sspsiclgls
       61 fdslrggktt vedqrcnvkr hglrkqaile catagdvkmm helgglghfc gledhilqlg
      121 vlplvatssa gdrvvagvfa acvafdscpp nasaaqkilt gshamatvhk rdyvvnfvnw
      181 frlvddvvlh cnlrlqtlle gwerlrvfsl pqlktliasa pdtgidnlfi apealralvs
      241 scpqllqhcr lplmq
//
LOCUS       XP_077502255             255 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113186 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502255
VERSION     XP_077502255.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646129.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..255
                     /product="uncharacterized protein LOC144113186 isoform X1"
                     /calculated_mol_wt=28018
     CDS             1..255
                     /gene="LOC144113186"
                     /coded_by="XM_077646129.1:27..794"
                     /db_xref="GeneID:144113186"
ORIGIN      
        1 mvtlqrahvk hvflpwcflq lremwppmhd llepcaplln mvltrlssll sspsiclgls
       61 fdslrggktt vedqrcnvkr hglrkqaile catagdvkmm helgglghfc gledhilqlg
      121 vlplvatssa gdrvvagvfa acvafdscpp nasaaqkilt gshamatvhk rdyvvnfvnw
      181 frlvddvvlh cnlrlqtlle gwerlrvfsl pqlktliasa pdtgidnlfi apealralvs
      241 scpqllqhcr lplmq
//
LOCUS       XP_077502256             244 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113186 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502256
VERSION     XP_077502256.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646130.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..244
                     /product="uncharacterized protein LOC144113186 isoform X2"
                     /calculated_mol_wt=26684
     CDS             1..244
                     /gene="LOC144113186"
                     /coded_by="XM_077646130.1:23..757"
                     /db_xref="GeneID:144113186"
ORIGIN      
        1 mvtlqrahvk hvflpwcflq lremwppmhd llepcaplln mvltrlssll sspsiclgls
       61 fdslrggktt vedqrcnvkr hglrkqaile catagdvkmm helgglghfc gledhilqlg
      121 vlplvatssa gdrvvagvfa acvafdscpp nasaaqkilt gshamatvhk rdyvvnfvnw
      181 frlvddvvlh cnlrlqtlle gwerlrvfsl pqlktliasa pdtgidnlfi apealralvs
      241 scpq
//
LOCUS       XP_077502257             232 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113186 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502257
VERSION     XP_077502257.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646131.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..232
                     /product="uncharacterized protein LOC144113186 isoform X3"
                     /calculated_mol_wt=25184
     CDS             1..232
                     /gene="LOC144113186"
                     /coded_by="XM_077646131.1:1979..2677"
                     /db_xref="GeneID:144113186"
ORIGIN      
        1 mwppmhdlle pcapllnmvl trlssllssp siclglsfds lrggkttved qrcnvkrhgl
       61 rkqailecat agdvkmmhel gglghfcgle dhilqlgvlp lvatssagdr vvagvfaacv
      121 afdscppnas aaqkiltgsh amatvhkrdy vvnfvnwfrl vddvvlhcnl rlqtllegwe
      181 rlrvfslpql ktliasapdt gidnlfiape alralvsscp qllqhcrlpl mq
//
LOCUS       XP_077502258             232 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113186 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502258
VERSION     XP_077502258.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646132.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..232
                     /product="uncharacterized protein LOC144113186 isoform X3"
                     /calculated_mol_wt=25184
     CDS             1..232
                     /gene="LOC144113186"
                     /coded_by="XM_077646132.1:1768..2466"
                     /db_xref="GeneID:144113186"
ORIGIN      
        1 mwppmhdlle pcapllnmvl trlssllssp siclglsfds lrggkttved qrcnvkrhgl
       61 rkqailecat agdvkmmhel gglghfcgle dhilqlgvlp lvatssagdr vvagvfaacv
      121 afdscppnas aaqkiltgsh amatvhkrdy vvnfvnwfrl vddvvlhcnl rlqtllegwe
      181 rlrvfslpql ktliasapdt gidnlfiape alralvsscp qllqhcrlpl mq
//
LOCUS       XP_077502259             223 aa            linear   INV 25-NOV-2025
DEFINITION  protein regulator of cytokinesis 1-like isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077502259
VERSION     XP_077502259.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646133.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..223
                     /product="protein regulator of cytokinesis 1-like isoform
                     X4"
                     /calculated_mol_wt=25699
     Region          <111..218
                     /region_name="MAP65_ASE1"
                     /note="Microtubule associated protein (MAP65/ASE1 family);
                     pfam03999"
                     /db_xref="CDD:427641"
     CDS             1..223
                     /gene="LOC144113186"
                     /coded_by="XM_077646133.1:97..768"
                     /db_xref="GeneID:144113186"
ORIGIN      
        1 mpewraamag prtsakqlap rdvathvrps tpcapllnmv ltrlssllss psiclglsfd
       61 slrggkttve dqrcnvkrhg lrkqailecg tagdfsdrrl eeleeelrrl kefhaknkpi
      121 lmkverreal warflefekr aadpfrlsnr ggslllemre rkrleaelpr leeeikthis
      181 ghtgvedeqf ftewaedflk hlssqheayn lekdrerqer tpr
//
LOCUS       XP_077502260             223 aa            linear   INV 25-NOV-2025
DEFINITION  protein regulator of cytokinesis 1-like isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077502260
VERSION     XP_077502260.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646134.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..223
                     /product="protein regulator of cytokinesis 1-like isoform
                     X4"
                     /calculated_mol_wt=25699
     Region          <111..218
                     /region_name="MAP65_ASE1"
                     /note="Microtubule associated protein (MAP65/ASE1 family);
                     pfam03999"
                     /db_xref="CDD:427641"
     CDS             1..223
                     /gene="LOC144113186"
                     /coded_by="XM_077646134.1:97..768"
                     /db_xref="GeneID:144113186"
ORIGIN      
        1 mpewraamag prtsakqlap rdvathvrps tpcapllnmv ltrlssllss psiclglsfd
       61 slrggkttve dqrcnvkrhg lrkqailecg tagdfsdrrl eeleeelrrl kefhaknkpi
      121 lmkverreal warflefekr aadpfrlsnr ggslllemre rkrleaelpr leeeikthis
      181 ghtgvedeqf ftewaedflk hlssqheayn lekdrerqer tpr
//
LOCUS       XP_077502261             221 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113186 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077502261
VERSION     XP_077502261.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646135.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..221
                     /product="uncharacterized protein LOC144113186 isoform X5"
                     /calculated_mol_wt=23850
     CDS             1..221
                     /gene="LOC144113186"
                     /coded_by="XM_077646135.1:1988..2653"
                     /db_xref="GeneID:144113186"
ORIGIN      
        1 mwppmhdlle pcapllnmvl trlssllssp siclglsfds lrggkttved qrcnvkrhgl
       61 rkqailecat agdvkmmhel gglghfcgle dhilqlgvlp lvatssagdr vvagvfaacv
      121 afdscppnas aaqkiltgsh amatvhkrdy vvnfvnwfrl vddvvlhcnl rlqtllegwe
      181 rlrvfslpql ktliasapdt gidnlfiape alralvsscp q
//
LOCUS       XP_077502262             241 aa            linear   INV 25-NOV-2025
DEFINITION  protein regulator of cytokinesis 1-like [Amblyomma americanum].
ACCESSION   XP_077502262
VERSION     XP_077502262.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646136.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..241
                     /product="protein regulator of cytokinesis 1-like"
                     /calculated_mol_wt=27925
     Region          <117..225
                     /region_name="MAP65_ASE1"
                     /note="Microtubule associated protein (MAP65/ASE1 family);
                     pfam03999"
                     /db_xref="CDD:427641"
     CDS             1..241
                     /gene="LOC144113188"
                     /coded_by="XM_077646136.1:1695..2420"
                     /db_xref="GeneID:144113188"
ORIGIN      
        1 mapatdivww pggimdfkmm helggldnfg gleddiliss psrgngasql ssslhftrgl
       61 hledhgqyat ttaesfvsrs crlplrhsae crismhgaed fsdqrleele eelrrlkefh
      121 aknkpilmkv erqealwarf vefekraadp frlnncggrl llemrerkrl eaelprleee
      181 ikthisrhts vedeqfftew aedflkhlss qheaynledr erqertfqig glknwrksse
      241 g
//
LOCUS       XP_077502263             213 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113189 [Amblyomma americanum].
ACCESSION   XP_077502263
VERSION     XP_077502263.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646137.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..213
                     /product="uncharacterized protein LOC144113189"
                     /calculated_mol_wt=24325
     CDS             1..213
                     /gene="LOC144113189"
                     /coded_by="XM_077646137.1:3335..3976"
                     /db_xref="GeneID:144113189"
ORIGIN      
        1 mmhelggldt prrlrlfpai hrpeflvdah alvtghksys vvncvnwfrl agdlvrhhel
       61 rlqafpagwe rlrgsgqphl eahhrnmmhe lggldtprrl rlfpaihrpe flvdahalvt
      121 ghksysvvnc vnwfrlagdl vrhhelrlqa fpagwerlrg sgqphleahr rnmmhklgcl
      181 dtsprlrplp svhlwrpefv adaqamvtgd ksn
//
LOCUS       XP_077502264             213 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113189 [Amblyomma americanum].
ACCESSION   XP_077502264
VERSION     XP_077502264.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646138.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..213
                     /product="uncharacterized protein LOC144113189"
                     /calculated_mol_wt=24325
     CDS             1..213
                     /gene="LOC144113189"
                     /coded_by="XM_077646138.1:3179..3820"
                     /db_xref="GeneID:144113189"
ORIGIN      
        1 mmhelggldt prrlrlfpai hrpeflvdah alvtghksys vvncvnwfrl agdlvrhhel
       61 rlqafpagwe rlrgsgqphl eahhrnmmhe lggldtprrl rlfpaihrpe flvdahalvt
      121 ghksysvvnc vnwfrlagdl vrhhelrlqa fpagwerlrg sgqphleahr rnmmhklgcl
      181 dtsprlrplp svhlwrpefv adaqamvtgd ksn
//
LOCUS       XP_077502265             213 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113189 [Amblyomma americanum].
ACCESSION   XP_077502265
VERSION     XP_077502265.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646139.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..213
                     /product="uncharacterized protein LOC144113189"
                     /calculated_mol_wt=24325
     CDS             1..213
                     /gene="LOC144113189"
                     /coded_by="XM_077646139.1:2747..3388"
                     /db_xref="GeneID:144113189"
ORIGIN      
        1 mmhelggldt prrlrlfpai hrpeflvdah alvtghksys vvncvnwfrl agdlvrhhel
       61 rlqafpagwe rlrgsgqphl eahhrnmmhe lggldtprrl rlfpaihrpe flvdahalvt
      121 ghksysvvnc vnwfrlagdl vrhhelrlqa fpagwerlrg sgqphleahr rnmmhklgcl
      181 dtsprlrplp svhlwrpefv adaqamvtgd ksn
//
LOCUS       XP_077502267             213 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113189 [Amblyomma americanum].
ACCESSION   XP_077502267
VERSION     XP_077502267.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646141.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..213
                     /product="uncharacterized protein LOC144113189"
                     /calculated_mol_wt=24325
     CDS             1..213
                     /gene="LOC144113189"
                     /coded_by="XM_077646141.1:3180..3821"
                     /db_xref="GeneID:144113189"
ORIGIN      
        1 mmhelggldt prrlrlfpai hrpeflvdah alvtghksys vvncvnwfrl agdlvrhhel
       61 rlqafpagwe rlrgsgqphl eahhrnmmhe lggldtprrl rlfpaihrpe flvdahalvt
      121 ghksysvvnc vnwfrlagdl vrhhelrlqa fpagwerlrg sgqphleahr rnmmhklgcl
      181 dtsprlrplp svhlwrpefv adaqamvtgd ksn
//
LOCUS       XP_077502268             213 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113189 [Amblyomma americanum].
ACCESSION   XP_077502268
VERSION     XP_077502268.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646142.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..213
                     /product="uncharacterized protein LOC144113189"
                     /calculated_mol_wt=24325
     CDS             1..213
                     /gene="LOC144113189"
                     /coded_by="XM_077646142.1:3438..4079"
                     /db_xref="GeneID:144113189"
ORIGIN      
        1 mmhelggldt prrlrlfpai hrpeflvdah alvtghksys vvncvnwfrl agdlvrhhel
       61 rlqafpagwe rlrgsgqphl eahhrnmmhe lggldtprrl rlfpaihrpe flvdahalvt
      121 ghksysvvnc vnwfrlagdl vrhhelrlqa fpagwerlrg sgqphleahr rnmmhklgcl
      181 dtsprlrplp svhlwrpefv adaqamvtgd ksn
//
LOCUS       XP_077502269             213 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113189 [Amblyomma americanum].
ACCESSION   XP_077502269
VERSION     XP_077502269.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646143.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..213
                     /product="uncharacterized protein LOC144113189"
                     /calculated_mol_wt=24325
     CDS             1..213
                     /gene="LOC144113189"
                     /coded_by="XM_077646143.1:3438..4079"
                     /db_xref="GeneID:144113189"
ORIGIN      
        1 mmhelggldt prrlrlfpai hrpeflvdah alvtghksys vvncvnwfrl agdlvrhhel
       61 rlqafpagwe rlrgsgqphl eahhrnmmhe lggldtprrl rlfpaihrpe flvdahalvt
      121 ghksysvvnc vnwfrlagdl vrhhelrlqa fpagwerlrg sgqphleahr rnmmhklgcl
      181 dtsprlrplp svhlwrpefv adaqamvtgd ksn
//
LOCUS       XP_077502270             213 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113189 [Amblyomma americanum].
ACCESSION   XP_077502270
VERSION     XP_077502270.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646144.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..213
                     /product="uncharacterized protein LOC144113189"
                     /calculated_mol_wt=24325
     CDS             1..213
                     /gene="LOC144113189"
                     /coded_by="XM_077646144.1:2376..3017"
                     /db_xref="GeneID:144113189"
ORIGIN      
        1 mmhelggldt prrlrlfpai hrpeflvdah alvtghksys vvncvnwfrl agdlvrhhel
       61 rlqafpagwe rlrgsgqphl eahhrnmmhe lggldtprrl rlfpaihrpe flvdahalvt
      121 ghksysvvnc vnwfrlagdl vrhhelrlqa fpagwerlrg sgqphleahr rnmmhklgcl
      181 dtsprlrplp svhlwrpefv adaqamvtgd ksn
//
LOCUS       XP_077502271             213 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113189 [Amblyomma americanum].
ACCESSION   XP_077502271
VERSION     XP_077502271.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646145.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..213
                     /product="uncharacterized protein LOC144113189"
                     /calculated_mol_wt=24325
     CDS             1..213
                     /gene="LOC144113189"
                     /coded_by="XM_077646145.1:943..1584"
                     /db_xref="GeneID:144113189"
ORIGIN      
        1 mmhelggldt prrlrlfpai hrpeflvdah alvtghksys vvncvnwfrl agdlvrhhel
       61 rlqafpagwe rlrgsgqphl eahhrnmmhe lggldtprrl rlfpaihrpe flvdahalvt
      121 ghksysvvnc vnwfrlagdl vrhhelrlqa fpagwerlrg sgqphleahr rnmmhklgcl
      181 dtsprlrplp svhlwrpefv adaqamvtgd ksn
//
LOCUS       XP_077502272             213 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113189 [Amblyomma americanum].
ACCESSION   XP_077502272
VERSION     XP_077502272.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646146.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..213
                     /product="uncharacterized protein LOC144113189"
                     /calculated_mol_wt=24325
     CDS             1..213
                     /gene="LOC144113189"
                     /coded_by="XM_077646146.1:164..805"
                     /db_xref="GeneID:144113189"
ORIGIN      
        1 mmhelggldt prrlrlfpai hrpeflvdah alvtghksys vvncvnwfrl agdlvrhhel
       61 rlqafpagwe rlrgsgqphl eahhrnmmhe lggldtprrl rlfpaihrpe flvdahalvt
      121 ghksysvvnc vnwfrlagdl vrhhelrlqa fpagwerlrg sgqphleahr rnmmhklgcl
      181 dtsprlrplp svhlwrpefv adaqamvtgd ksn
//
LOCUS       XP_077502273             213 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113189 [Amblyomma americanum].
ACCESSION   XP_077502273
VERSION     XP_077502273.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646147.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..213
                     /product="uncharacterized protein LOC144113189"
                     /calculated_mol_wt=24325
     CDS             1..213
                     /gene="LOC144113189"
                     /coded_by="XM_077646147.1:668..1309"
                     /db_xref="GeneID:144113189"
ORIGIN      
        1 mmhelggldt prrlrlfpai hrpeflvdah alvtghksys vvncvnwfrl agdlvrhhel
       61 rlqafpagwe rlrgsgqphl eahhrnmmhe lggldtprrl rlfpaihrpe flvdahalvt
      121 ghksysvvnc vnwfrlagdl vrhhelrlqa fpagwerlrg sgqphleahr rnmmhklgcl
      181 dtsprlrplp svhlwrpefv adaqamvtgd ksn
//
LOCUS       XP_077502274             153 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113190 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502274
VERSION     XP_077502274.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646148.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..153
                     /product="uncharacterized protein LOC144113190 isoform X1"
                     /calculated_mol_wt=17974
     Region          <57..105
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     CDS             1..153
                     /gene="LOC144113190"
                     /coded_by="XM_077646148.1:143..604"
                     /db_xref="GeneID:144113190"
ORIGIN      
        1 mmerkrleae lprleeeikt hisrhtgved eqfftewaed flkhlssqhe afnlekdrer
       61 qerlvqrcrl plmrreflls kvsdeplvrt skeskdllie amryhltplp plsgwvmnrt
      121 direqghctt ssrlacflam mfpaaprdad tha
//
LOCUS       XP_077502275             152 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113190 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502275
VERSION     XP_077502275.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646149.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..152
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..152
                     /product="uncharacterized protein LOC144113190 isoform X2"
                     /calculated_mol_wt=17891
     Region          <56..104
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     CDS             1..152
                     /gene="LOC144113190"
                     /coded_by="XM_077646149.1:190..648"
                     /db_xref="GeneID:144113190"
ORIGIN      
        1 mmerkrleae lprleeeikt hisrhtsved eqfftewaed flkhlssqhe aynledrerq
       61 erlvqrcrlp lmrrefllsk vsdeplvrts keskdlliea mryhltplpp lsgwvmnrtd
      121 ireqghctts srlacflamm fpaaprdadt ha
//
LOCUS       XP_077502276             146 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113190 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502276
VERSION     XP_077502276.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646150.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..146
                     /product="uncharacterized protein LOC144113190 isoform X3"
                     /calculated_mol_wt=17318
     Region          <57..105
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     CDS             1..146
                     /gene="LOC144113190"
                     /coded_by="XM_077646150.1:143..583"
                     /db_xref="GeneID:144113190"
ORIGIN      
        1 mmerkrleae lprleeeikt hisrhtgved eqfftewaed flkhlssqhe afnlekdrer
       61 qerlvqrcrl plmrreflls kvsdeplvrt skeskdllie amryhltplp plsgvfsrhd
      121 vscssarcrh pcmtfwslli hcltrc
//
LOCUS       XP_077502277             136 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113190 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077502277
VERSION     XP_077502277.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646151.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..136
                     /product="uncharacterized protein LOC144113190 isoform X4"
                     /calculated_mol_wt=16200
     Region          <57..105
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     CDS             1..136
                     /gene="LOC144113190"
                     /coded_by="XM_077646151.1:143..553"
                     /db_xref="GeneID:144113190"
ORIGIN      
        1 mmerkrleae lprleeeikt hisrhtgved eqfftewaed flkhlssqhe afnlekdrer
       61 qerlvqrcrl plmrreflls kvsdeplvrt skeskdllie amryhltplp plsgsarcrh
      121 pcmtfwslli hcltrc
//
LOCUS       XP_077502278             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113193 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502278
VERSION     XP_077502278.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646152.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144113193 isoform X1"
                     /calculated_mol_wt=14345
     CDS             1..127
                     /gene="LOC144113193"
                     /coded_by="XM_077646152.1:6252..6635"
                     /db_xref="GeneID:144113193"
ORIGIN      
        1 mmhelggldt prrlrlfpai hrpeflvdah alvtghksys vvncvnwfrl agdlvrhhel
       61 rlqafpagwe rlrgsgqphl eahrrnmmhk lggldtsprl rplpsvhlwh pefvadaqam
      121 vtgdksn
//
LOCUS       XP_077502279             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113193 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502279
VERSION     XP_077502279.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646153.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144113193 isoform X1"
                     /calculated_mol_wt=14345
     CDS             1..127
                     /gene="LOC144113193"
                     /coded_by="XM_077646153.1:6252..6635"
                     /db_xref="GeneID:144113193"
ORIGIN      
        1 mmhelggldt prrlrlfpai hrpeflvdah alvtghksys vvncvnwfrl agdlvrhhel
       61 rlqafpagwe rlrgsgqphl eahrrnmmhk lggldtsprl rplpsvhlwh pefvadaqam
      121 vtgdksn
//
LOCUS       XP_077502280             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113193 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502280
VERSION     XP_077502280.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646154.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144113193 isoform X1"
                     /calculated_mol_wt=14345
     CDS             1..127
                     /gene="LOC144113193"
                     /coded_by="XM_077646154.1:5984..6367"
                     /db_xref="GeneID:144113193"
ORIGIN      
        1 mmhelggldt prrlrlfpai hrpeflvdah alvtghksys vvncvnwfrl agdlvrhhel
       61 rlqafpagwe rlrgsgqphl eahrrnmmhk lggldtsprl rplpsvhlwh pefvadaqam
      121 vtgdksn
//
LOCUS       XP_077502281              83 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113193 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502281
VERSION     XP_077502281.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646155.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..83
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..83
                     /product="uncharacterized protein LOC144113193 isoform X2"
                     /calculated_mol_wt=8848
     CDS             1..83
                     /gene="LOC144113193"
                     /coded_by="XM_077646155.1:5076..5327"
                     /db_xref="GeneID:144113193"
ORIGIN      
        1 mmhelgrldn lggleddili slpsrgngas qlssslhmpr glhledhgqy atttaesfva
       61 rvdllcvtll nagfrsmvqk tai
//
LOCUS       XP_077502282             171 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113194 [Amblyomma americanum].
ACCESSION   XP_077502282
VERSION     XP_077502282.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646156.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..171
                     /product="uncharacterized protein LOC144113194"
                     /calculated_mol_wt=17869
     CDS             1..171
                     /gene="LOC144113194"
                     /coded_by="XM_077646156.1:136..651"
                     /db_xref="GeneID:144113194"
ORIGIN      
        1 mavvigqpav pnslpketsv aplqectalc pmssvdplsl psgsvqcrli speilastlr
       61 mlssssaiss psgatarspf ppaswpslad nsfytwqhgs stisdmgdty knkpgtthlg
      121 hgtvtsmeil plqilqlsat krrghwvsha vacatllapf valpnsshrt h
//
LOCUS       XP_077502283             174 aa            linear   INV 25-NOV-2025
DEFINITION  natterin-4-like [Amblyomma americanum].
ACCESSION   XP_077502283
VERSION     XP_077502283.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646157.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..174
                     /product="natterin-4-like"
                     /calculated_mol_wt=18957
     Region          50..161
                     /region_name="DUF3421"
                     /note="Protein of unknown function (DUF3421); pfam11901"
                     /db_xref="CDD:463390"
     CDS             1..174
                     /gene="LOC144113195"
                     /coded_by="XM_077646157.1:70..594"
                     /db_xref="GeneID:144113195"
ORIGIN      
        1 mrpipleild ceeayqhdgs hmgvqrlcyw krcrgghvpy navcggedlg geslyvgras
       61 hsgdlvpgkl vpshgvcyvs wgegehghdn yevlvsndal lqwlrasngs vpsgaiqggv
      121 tvtgerlyig rarhhntltl gkvhpshgcl ylpfagnehr yteyealvcm tinf
//
LOCUS       XP_077502284             194 aa            linear   INV 25-NOV-2025
DEFINITION  natterin-4-like [Amblyomma americanum].
ACCESSION   XP_077502284
VERSION     XP_077502284.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646158.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..194
                     /product="natterin-4-like"
                     /calculated_mol_wt=20871
     Region          71..181
                     /region_name="DUF3421"
                     /note="Protein of unknown function (DUF3421); pfam11901"
                     /db_xref="CDD:463390"
     CDS             1..194
                     /gene="LOC144113196"
                     /coded_by="XM_077646158.1:36..620"
                     /db_xref="GeneID:144113196"
ORIGIN      
        1 mqtlallrrl srsiygptlt rerhpasnqq ltlytkmagf gafhpqpvcq wvqchgsrlp
       61 snavpggedc gesifigram hggdlvpgkv vpshdccyvs ydgtehshqe yqalvtngtp
      121 mawvpaaggs vptgaiqggs cangeplyig rtfhdgtlti gkvhpshgcl yipyggqehr
      181 yteyevlvck tinf
//
LOCUS       XP_077502285             192 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113197 [Amblyomma americanum].
ACCESSION   XP_077502285
VERSION     XP_077502285.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646159.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..192
                     /product="uncharacterized protein LOC144113197"
                     /calculated_mol_wt=20506
     Region          4..88
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     CDS             1..192
                     /gene="LOC144113197"
                     /coded_by="XM_077646159.1:19..597"
                     /db_xref="GeneID:144113197"
ORIGIN      
        1 mmeqfletvr vfpflydkth peykdkdakm nrwaiigrtf dltgpqaaak fknvkdrwlk
       61 ivsasegsrk sgaggeagkv htkwtlfdiv dgmlrstphy aersssnvpp eedisiltsp
      121 lssncpsspr lvascisspa aalfqemysd spndftdedd lrlpstsega asaagssgls
      181 tsqagaaapd pg
//
LOCUS       XP_077502287             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113202 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502287
VERSION     XP_077502287.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646161.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144113202 isoform X1"
                     /calculated_mol_wt=14525
     CDS             1..133
                     /gene="LOC144113202"
                     /coded_by="XM_077646161.1:434..835"
                     /db_xref="GeneID:144113202"
ORIGIN      
        1 mlrrpvtikr igkcsilmnt fircenccig snslfkdchl easevviann cyingltvnv
       61 kdealvipan scvlqkesrl enfntapvvi afgmddhlgv eemqpaffsg cvgtpesegp
      121 vsqvlehlfs cwt
//
LOCUS       XP_077502288             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113202 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502288
VERSION     XP_077502288.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646162.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144113202 isoform X1"
                     /calculated_mol_wt=14525
     CDS             1..133
                     /gene="LOC144113202"
                     /coded_by="XM_077646162.1:2175..2576"
                     /db_xref="GeneID:144113202"
ORIGIN      
        1 mlrrpvtikr igkcsilmnt fircenccig snslfkdchl easevviann cyingltvnv
       61 kdealvipan scvlqkesrl enfntapvvi afgmddhlgv eemqpaffsg cvgtpesegp
      121 vsqvlehlfs cwt
//
LOCUS       XP_077502289             130 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113202 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502289
VERSION     XP_077502289.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646163.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..130
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..130
                     /product="uncharacterized protein LOC144113202 isoform X2"
                     /calculated_mol_wt=14115
     CDS             1..130
                     /gene="LOC144113202"
                     /coded_by="XM_077646163.1:251..643"
                     /db_xref="GeneID:144113202"
ORIGIN      
        1 mlvtikrigk csilmntfir cenccigsns lfkdchleas evvianncyi ngltvnvkde
       61 alvipanscv lqkesrlenf ntapvviafg mddhlgveem qpaffsgcvg tpesegpvsq
      121 vlehlfscwt
//
LOCUS       XP_077502290             100 aa            linear   INV 25-NOV-2025
DEFINITION  L-fucose kinase-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077502290
VERSION     XP_077502290.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646164.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..100
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..100
                     /product="L-fucose kinase-like isoform X3"
                     /calculated_mol_wt=10526
     Region          <1..>88
                     /region_name="fkp"
                     /note="bifunctional
                     fucokinase/L-fucose-1-P-guanylyltransferase; Provisional;
                     PRK13412"
                     /db_xref="CDD:237379"
     CDS             1..100
                     /gene="LOC144113202"
                     /coded_by="XM_077646164.1:2692..2994"
                     /db_xref="GeneID:144113202"
ORIGIN      
        1 mdlfggctdt ppigyelggs viniavlvdg qkpigakarr lhephivikl lhdlvpekie
       61 itsiedlldy sqpgargall kaclvgsgvv eidsknklsq
//
LOCUS       XP_077502291             100 aa            linear   INV 25-NOV-2025
DEFINITION  L-fucose kinase-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077502291
VERSION     XP_077502291.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646165.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..100
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..100
                     /product="L-fucose kinase-like isoform X3"
                     /calculated_mol_wt=10526
     Region          <1..>88
                     /region_name="fkp"
                     /note="bifunctional
                     fucokinase/L-fucose-1-P-guanylyltransferase; Provisional;
                     PRK13412"
                     /db_xref="CDD:237379"
     CDS             1..100
                     /gene="LOC144113202"
                     /coded_by="XM_077646165.1:1661..1963"
                     /db_xref="GeneID:144113202"
ORIGIN      
        1 mdlfggctdt ppigyelggs viniavlvdg qkpigakarr lhephivikl lhdlvpekie
       61 itsiedlldy sqpgargall kaclvgsgvv eidsknklsq
//
LOCUS       XP_077502292             997 aa            linear   INV 25-NOV-2025
DEFINITION  oxysterol-binding protein-related protein 6-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077502292
VERSION     XP_077502292.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646166.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..997
                     /product="oxysterol-binding protein-related protein 6-like
                     isoform X1"
                     /calculated_mol_wt=110878
     Region          84..206
                     /region_name="PH_ORP3_ORP6_ORP7"
                     /note="Human Oxysterol binding protein related proteins 3,
                     6, and 7 Pleckstrin homology (PH) domain; cd13287"
                     /db_xref="CDD:270104"
     Region          630..979
                     /region_name="Oxysterol_BP"
                     /note="Oxysterol-binding protein; pfam01237"
                     /db_xref="CDD:460126"
     CDS             1..997
                     /gene="LOC144113206"
                     /coded_by="XM_077646166.1:606..3599"
                     /db_xref="GeneID:144113206"
ORIGIN      
        1 meepqpemat tttaaaaatt naatvatttt tttttnnsds easpgrsspa sqqsepgsra
       61 apdvaairpe apaattagss rrrraadwei mqglrtgqrc ddkprkfegy llkrrkwplk
      121 gwhkrffcle kgiltyakss tdmakgknhg svdlglsvis tkskgrridi daeefiyhlk
      181 vknqaqfqqw vsqlqhhrly rqheitfgsr eapkmtspaa edsatlpgan gpsgfelssl
      241 sspmllensr lirsplnqqq srvaawilds saidhgnkda smlqeklfhl anllqliens
      301 pstvssnyle ipemehvvgg qkkdrkrfhl krirrgkkds dpsvankggs sgssssgkpp
      361 lnveitvsqd aagtndifas mdnlteanrs dksdgsamls irdmgylscs hpslasdgtl
      421 vgpvpdttsl aaycsydtta vfkhmeqfls lakevhsglr tmlrslqter erlkqhlese
      481 ttascgsnaa lianlknalt qavqqnydlr aclkkvheet dpsafpapdg saaesfdept
      541 lvhqpvqqsv shesssalst seffdaaekl slssssseee eeegsfcsev sddgteyapg
      601 aegnlrdhlp ltqgrrsklp apkpdagdts lwnllcknig kdlskvsmpv tineplnmlq
      661 rlceeleysd lldkaaated hfdrmvciaa favsgysssy hraghkpfnp llgetyecir
      721 edkgfrfiae qvnhhppvsa cyaesknykf wqdvriktkf wgksmeilpv gtvhlellss
      781 gshyqwnkvt tcvhnifsgq rwvdqygemt irdlthdvtc kltfvkasyw sekrhevfgn
      841 vvnkqgdvif nlsgqwteai ycgvaptakc iwrpgtmped yelyygftrf aielneldpq
      901 makvlpptds rfrpdqrlle egnigaaeav ksqleqnqre rrkqreemgi dyqpmwfrke
      961 ttkdgkehwt ythkywetrt npgfgsisfp eklwdpl
//
LOCUS       XP_077502293             997 aa            linear   INV 25-NOV-2025
DEFINITION  oxysterol-binding protein-related protein 6-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077502293
VERSION     XP_077502293.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646167.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..997
                     /product="oxysterol-binding protein-related protein 6-like
                     isoform X1"
                     /calculated_mol_wt=110878
     Region          84..206
                     /region_name="PH_ORP3_ORP6_ORP7"
                     /note="Human Oxysterol binding protein related proteins 3,
                     6, and 7 Pleckstrin homology (PH) domain; cd13287"
                     /db_xref="CDD:270104"
     Region          630..979
                     /region_name="Oxysterol_BP"
                     /note="Oxysterol-binding protein; pfam01237"
                     /db_xref="CDD:460126"
     CDS             1..997
                     /gene="LOC144113206"
                     /coded_by="XM_077646167.1:195..3188"
                     /db_xref="GeneID:144113206"
ORIGIN      
        1 meepqpemat tttaaaaatt naatvatttt tttttnnsds easpgrsspa sqqsepgsra
       61 apdvaairpe apaattagss rrrraadwei mqglrtgqrc ddkprkfegy llkrrkwplk
      121 gwhkrffcle kgiltyakss tdmakgknhg svdlglsvis tkskgrridi daeefiyhlk
      181 vknqaqfqqw vsqlqhhrly rqheitfgsr eapkmtspaa edsatlpgan gpsgfelssl
      241 sspmllensr lirsplnqqq srvaawilds saidhgnkda smlqeklfhl anllqliens
      301 pstvssnyle ipemehvvgg qkkdrkrfhl krirrgkkds dpsvankggs sgssssgkpp
      361 lnveitvsqd aagtndifas mdnlteanrs dksdgsamls irdmgylscs hpslasdgtl
      421 vgpvpdttsl aaycsydtta vfkhmeqfls lakevhsglr tmlrslqter erlkqhlese
      481 ttascgsnaa lianlknalt qavqqnydlr aclkkvheet dpsafpapdg saaesfdept
      541 lvhqpvqqsv shesssalst seffdaaekl slssssseee eeegsfcsev sddgteyapg
      601 aegnlrdhlp ltqgrrsklp apkpdagdts lwnllcknig kdlskvsmpv tineplnmlq
      661 rlceeleysd lldkaaated hfdrmvciaa favsgysssy hraghkpfnp llgetyecir
      721 edkgfrfiae qvnhhppvsa cyaesknykf wqdvriktkf wgksmeilpv gtvhlellss
      781 gshyqwnkvt tcvhnifsgq rwvdqygemt irdlthdvtc kltfvkasyw sekrhevfgn
      841 vvnkqgdvif nlsgqwteai ycgvaptakc iwrpgtmped yelyygftrf aielneldpq
      901 makvlpptds rfrpdqrlle egnigaaeav ksqleqnqre rrkqreemgi dyqpmwfrke
      961 ttkdgkehwt ythkywetrt npgfgsisfp eklwdpl
//
LOCUS       XP_077502294             990 aa            linear   INV 25-NOV-2025
DEFINITION  oxysterol-binding protein-related protein 6-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077502294
VERSION     XP_077502294.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646168.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..990
                     /product="oxysterol-binding protein-related protein 6-like
                     isoform X2"
                     /calculated_mol_wt=110076
     Region          84..206
                     /region_name="PH_ORP3_ORP6_ORP7"
                     /note="Human Oxysterol binding protein related proteins 3,
                     6, and 7 Pleckstrin homology (PH) domain; cd13287"
                     /db_xref="CDD:270104"
     Region          623..972
                     /region_name="Oxysterol_BP"
                     /note="Oxysterol-binding protein; pfam01237"
                     /db_xref="CDD:460126"
     CDS             1..990
                     /gene="LOC144113206"
                     /coded_by="XM_077646168.1:576..3548"
                     /db_xref="GeneID:144113206"
ORIGIN      
        1 meepqpemat tttaaaaatt naatvatttt tttttnnsds easpgrsspa sqqsepgsra
       61 apdvaairpe apaattagss rrrraadwei mqglrtgqrc ddkprkfegy llkrrkwplk
      121 gwhkrffcle kgiltyakss tdmakgknhg svdlglsvis tkskgrridi daeefiyhlk
      181 vknqaqfqqw vsqlqhhrly rqheitfgsr eapkmtspaa edsatlpgan gpsgfelssl
      241 sspmllensr lirsplnqqq srvaawilds saidhgnkda smlqeklfhl anllqliens
      301 pstvssnyle ipemehvvgg qkkdrkrfhl krirrgkkds dpsvankggs sgssssgkpp
      361 lnveitvsqd aagtndifas mdnlteanrs dksdgsamls irdmgylscs hpslasdgtl
      421 vgpvpdttsl aaycsydtta vfkhmeqfls lakevhsglr tmlrslqter erlkqhlese
      481 ttascgsnaa lianlknalt qavqqnydlr aclkkvheet dpsafpapdg saaesfdept
      541 qsvshesssa lstseffdaa eklslsssss eeeeeegsfc sevsddgtey apgaegnlrd
      601 hlpltqgrrs klpapkpdag dtslwnllck nigkdlskvs mpvtinepln mlqrlceele
      661 ysdlldkaaa tedhfdrmvc iaafavsgys ssyhraghkp fnpllgetye ciredkgfrf
      721 iaeqvnhhpp vsacyaeskn ykfwqdvrik tkfwgksmei lpvgtvhlel lssgshyqwn
      781 kvttcvhnif sgqrwvdqyg emtirdlthd vtckltfvka sywsekrhev fgnvvnkqgd
      841 vifnlsgqwt eaiycgvapt akciwrpgtm pedyelyygf trfaielnel dpqmakvlpp
      901 tdsrfrpdqr lleegnigaa eavksqleqn qrerrkqree mgidyqpmwf rkettkdgke
      961 hwtythkywe trtnpgfgsi sfpeklwdpl
//
LOCUS       XP_077502295             990 aa            linear   INV 25-NOV-2025
DEFINITION  oxysterol-binding protein-related protein 6-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077502295
VERSION     XP_077502295.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646169.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..990
                     /product="oxysterol-binding protein-related protein 6-like
                     isoform X2"
                     /calculated_mol_wt=110076
     Region          84..206
                     /region_name="PH_ORP3_ORP6_ORP7"
                     /note="Human Oxysterol binding protein related proteins 3,
                     6, and 7 Pleckstrin homology (PH) domain; cd13287"
                     /db_xref="CDD:270104"
     Region          623..972
                     /region_name="Oxysterol_BP"
                     /note="Oxysterol-binding protein; pfam01237"
                     /db_xref="CDD:460126"
     CDS             1..990
                     /gene="LOC144113206"
                     /coded_by="XM_077646169.1:195..3167"
                     /db_xref="GeneID:144113206"
ORIGIN      
        1 meepqpemat tttaaaaatt naatvatttt tttttnnsds easpgrsspa sqqsepgsra
       61 apdvaairpe apaattagss rrrraadwei mqglrtgqrc ddkprkfegy llkrrkwplk
      121 gwhkrffcle kgiltyakss tdmakgknhg svdlglsvis tkskgrridi daeefiyhlk
      181 vknqaqfqqw vsqlqhhrly rqheitfgsr eapkmtspaa edsatlpgan gpsgfelssl
      241 sspmllensr lirsplnqqq srvaawilds saidhgnkda smlqeklfhl anllqliens
      301 pstvssnyle ipemehvvgg qkkdrkrfhl krirrgkkds dpsvankggs sgssssgkpp
      361 lnveitvsqd aagtndifas mdnlteanrs dksdgsamls irdmgylscs hpslasdgtl
      421 vgpvpdttsl aaycsydtta vfkhmeqfls lakevhsglr tmlrslqter erlkqhlese
      481 ttascgsnaa lianlknalt qavqqnydlr aclkkvheet dpsafpapdg saaesfdept
      541 qsvshesssa lstseffdaa eklslsssss eeeeeegsfc sevsddgtey apgaegnlrd
      601 hlpltqgrrs klpapkpdag dtslwnllck nigkdlskvs mpvtinepln mlqrlceele
      661 ysdlldkaaa tedhfdrmvc iaafavsgys ssyhraghkp fnpllgetye ciredkgfrf
      721 iaeqvnhhpp vsacyaeskn ykfwqdvrik tkfwgksmei lpvgtvhlel lssgshyqwn
      781 kvttcvhnif sgqrwvdqyg emtirdlthd vtckltfvka sywsekrhev fgnvvnkqgd
      841 vifnlsgqwt eaiycgvapt akciwrpgtm pedyelyygf trfaielnel dpqmakvlpp
      901 tdsrfrpdqr lleegnigaa eavksqleqn qrerrkqree mgidyqpmwf rkettkdgke
      961 hwtythkywe trtnpgfgsi sfpeklwdpl
//
LOCUS       XP_077502296             989 aa            linear   INV 25-NOV-2025
DEFINITION  oxysterol-binding protein-related protein 6-like isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077502296
VERSION     XP_077502296.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646170.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..989
                     /product="oxysterol-binding protein-related protein 6-like
                     isoform X3"
                     /calculated_mol_wt=109947
     Region          84..206
                     /region_name="PH_ORP3_ORP6_ORP7"
                     /note="Human Oxysterol binding protein related proteins 3,
                     6, and 7 Pleckstrin homology (PH) domain; cd13287"
                     /db_xref="CDD:270104"
     Region          622..971
                     /region_name="Oxysterol_BP"
                     /note="Oxysterol-binding protein; pfam01237"
                     /db_xref="CDD:460126"
     CDS             1..989
                     /gene="LOC144113206"
                     /coded_by="XM_077646170.1:219..3188"
                     /db_xref="GeneID:144113206"
ORIGIN      
        1 meepqpemat tttaaaaatt naatvatttt tttttnnsds easpgrsspa sqqsepgsra
       61 apdvaairpe apaattagss rrrraadwei mqglrtgqrc ddkprkfegy llkrrkwplk
      121 gwhkrffcle kgiltyakss tdmakgknhg svdlglsvis tkskgrridi daeefiyhlk
      181 vknqaqfqqw vsqlqhhrly rqheitfgsr eapkmtspaa edsatlpgan gpsgfelssl
      241 sspmllensr lirsplnqqq srvaawilds saidhgnkda smlqeklfhl anllqliens
      301 pstvssnyle ipemehvvgg qkkdrkrfhl krirrgkkds dpsvankggs sgssssgkpp
      361 lnveitvsqd aagtndifas mdnlteanrs dksdgsamls irdmgylscs hpslasdgtl
      421 vgpvpdttsl aaycsydtta vfkhmeqfls lakevhsglr tmlrslqter erlkqhlese
      481 ttascgsnaa lianlknalt qavqqnydlr aclkkvheet dpsafpapdg saaesfdept
      541 svshesssal stseffdaae klslssssse eeeeegsfcs evsddgteya pgaegnlrdh
      601 lpltqgrrsk lpapkpdagd tslwnllckn igkdlskvsm pvtineplnm lqrlceeley
      661 sdlldkaaat edhfdrmvci aafavsgyss syhraghkpf npllgetyec iredkgfrfi
      721 aeqvnhhppv sacyaeskny kfwqdvrikt kfwgksmeil pvgtvhlell ssgshyqwnk
      781 vttcvhnifs gqrwvdqyge mtirdlthdv tckltfvkas ywsekrhevf gnvvnkqgdv
      841 ifnlsgqwte aiycgvapta kciwrpgtmp edyelyygft rfaielneld pqmakvlppt
      901 dsrfrpdqrl leegnigaae avksqleqnq rerrkqreem gidyqpmwfr kettkdgkeh
      961 wtythkywet rtnpgfgsis fpeklwdpl
//
LOCUS       XP_077502297             323 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113207 [Amblyomma americanum].
ACCESSION   XP_077502297
VERSION     XP_077502297.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646171.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..323
                     /product="uncharacterized protein LOC144113207"
                     /calculated_mol_wt=34528
     CDS             1..323
                     /gene="LOC144113207"
                     /coded_by="XM_077646171.1:124..1095"
                     /db_xref="GeneID:144113207"
ORIGIN      
        1 mqsnvpsprs pragtilltv revdpatwna lqqrpeglqi aqvtlkrgrd daappegvpe
       61 akrnnrsgpg vsderdadtl apergasrpp hpteaegcpd ergsaiesdg keeiplehnh
      121 aqegapdapd adpsgadgqg pdasvdqaam alldlrygwl arraipaagn gnpyeaiaep
      181 psaddrhsia plppdedspp rgisrellry alkssgsdiv rissptmlks llrprwkrcc
      241 laeggrcdvc akraitagag dndnecssvm apelqlpaiv vlskgkfhkm skdqasgppr
      301 eekmkrlegi vkklwikkae pml
//
LOCUS       XP_077502298             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113208 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502298
VERSION     XP_077502298.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646172.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144113208 isoform X1"
                     /calculated_mol_wt=15586
     CDS             1..141
                     /gene="LOC144113208"
                     /coded_by="XM_077646172.1:243..668"
                     /db_xref="GeneID:144113208"
ORIGIN      
        1 mtqaefrafr vgnmtavtlc tmclvgvgll llisgavvtg ivytevrppt adenydryrg
       61 adlrrvlgpl mlclggflli ggcvfmafgy ysnyrdeerg ysqahardyd sgqqpkdvkm
      121 rylgsssske dleagkrmme r
//
LOCUS       XP_077502299             123 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113208 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502299
VERSION     XP_077502299.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646173.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..123
                     /product="uncharacterized protein LOC144113208 isoform X2"
                     /calculated_mol_wt=13493
     CDS             1..123
                     /gene="LOC144113208"
                     /coded_by="XM_077646173.1:268..639"
                     /db_xref="GeneID:144113208"
ORIGIN      
        1 mtqaefrafr vgnmtavtlc tmclvgvgll llisgavvtg ivytevrppt adenydryrg
       61 adlrrvlgpl mlclggflli ggcvfmafgy ysnyrdeerg ysqahardyd sgqqpkdvkv
      121 sty
//
LOCUS       XP_077502300            1033 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: exosome RNA helicase MTR4-like [Amblyomma
            americanum].
ACCESSION   XP_077502300
VERSION     XP_077502300.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646174.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1033
                     /product="LOW QUALITY PROTEIN: exosome RNA helicase
                     MTR4-like"
                     /calculated_mol_wt=116787
     Region          120..1026
                     /region_name="Dob10"
                     /note="Superfamily II RNA helicase [Replication,
                     recombination and repair]; COG4581"
                     /db_xref="CDD:443638"
     CDS             1..1033
                     /gene="LOC144113209"
                     /coded_by="XM_077646174.1:110..3211"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 1 base in 1 codon"
                     /db_xref="GeneID:144113209"
ORIGIN      
        1 mddlfsvfdd apksskllss sclkkgdikk eestcedaga ndfvqavidg krsldnylee
       61 episkkqkas dirdefnaad ylprvhvhtv egvegcihev alpegteyvp lkrnadgpaa
      121 reypfildpf qkeaivclen nqsvlvsaht sagktvvaey aislalqekq rviyttpika
      181 lsnqkfrefs ddfkdvglmt gdvtinpsas climtteilr smlyrgseim revgwvifde
      241 ihymrdkerg vvweetiill pdnvryifls atipnarqfa ewichlhnqp cnvvyteyrp
      301 vplqhyifpa gasglylvvd etgnfkedkf neamavlqna gdaakgdstl xgrkggfkge
      361 sncykivkmi merdyapviv fsfskkecea yatqiakldl ttphekhlvq evfqnamdsl
      421 seedqklpqv eqvlpllkrg iavhhsgllp ilketieilf aeglvkalfa tetfamglnm
      481 partvlftna rkfdgkdfrw vtsgeyiqms gragrrgldd rgivilmvde kmspaagkdi
      541 vkglpdpins afhltynmvl nlmrveevnp eyilersffq fqnnamiptl yekllkleaq
      601 lesfqipneq evasyykvre qleslsqelq afltkpqycv pylqpgrmvy ihngeddfgw
      661 gaiinyskkk vvsargessl kepvviadil lnvskesaqq rltskvtppk pgekgemqvi
      721 pmpianirkv stirvfynqd lkssdnraav lksikeveer fpnnvplvdh fgslkindtk
      781 mrevvkkvea fekrmfahpv hsrqdsqkly eeykkkmkva qeirdvknel kkakallqmd
      841 elkcrkrvlr rlgystaadv meikgkvace issadellvt emifnntfne lnvhqatall
      901 sclvfqeksn empnlteels gplrqmqdia rrvaqvtkda klcidedtyv ssfkphlmda
      961 vyawskgssf aqvcrmtdvf egsiircmrr leellrqivq aakcignsel enkfseavkl
     1021 ikrdivfaas lyl
//
LOCUS       XP_077502301             277 aa            linear   INV 25-NOV-2025
DEFINITION  transmembrane protein 163a-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502301
VERSION     XP_077502301.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646175.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..277
                     /product="transmembrane protein 163a-like isoform X1"
                     /calculated_mol_wt=29969
     Region          84..>267
                     /region_name="FieF"
                     /note="Divalent metal cation (Fe/Co/Zn/Cd) efflux pump
                     [Inorganic ion transport and metabolism]; COG0053"
                     /db_xref="CDD:439823"
     CDS             1..277
                     /gene="LOC144113211"
                     /coded_by="XM_077646175.1:167..1000"
                     /db_xref="GeneID:144113211"
ORIGIN      
        1 mrptergkhn fgnscfvrvq lgwtaapkse lpcamerngl ltsgsngkvr vlnettplkl
       61 dsqllengpf eggdlnrkcv ltlsivsvvf nivvggtgfv fafqrnsasi yafaaeclld
      121 mlssmiviwq yltphysfar ervacillgl ffilsaiaii skamddlivr esptwipvli
      181 ilagvgaaac fllavvklil akrlgslslv ldginsliss fcavaiivsd lvydsnpevw
      241 ymdavlstva alimllvgak vlfenwsigr hghmtlq
//
LOCUS       XP_077502302             243 aa            linear   INV 25-NOV-2025
DEFINITION  transmembrane protein 163a-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502302
VERSION     XP_077502302.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646176.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..243
                     /product="transmembrane protein 163a-like isoform X2"
                     /calculated_mol_wt=26197
     Region          50..>233
                     /region_name="FieF"
                     /note="Divalent metal cation (Fe/Co/Zn/Cd) efflux pump
                     [Inorganic ion transport and metabolism]; COG0053"
                     /db_xref="CDD:439823"
     CDS             1..243
                     /gene="LOC144113211"
                     /coded_by="XM_077646176.1:463..1194"
                     /db_xref="GeneID:144113211"
ORIGIN      
        1 mernglltsg sngkvrvlne ttplkldsql lengpfeggd lnrkcvltls ivsvvfnivv
       61 ggtgfvfafq rnsasiyafa aeclldmlss miviwqyltp hysfarerva cillglffil
      121 saiaiiskam ddlivrespt wipvliilag vgaaacflla vvklilakrl gslslvldgi
      181 nslissfcav aiivsdlvyd snpevwymda vlstvaalim llvgakvlfe nwsigrhghm
      241 tlq
//
LOCUS       XP_077502303             243 aa            linear   INV 25-NOV-2025
DEFINITION  transmembrane protein 163a-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502303
VERSION     XP_077502303.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646177.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..243
                     /product="transmembrane protein 163a-like isoform X2"
                     /calculated_mol_wt=26197
     Region          50..>233
                     /region_name="FieF"
                     /note="Divalent metal cation (Fe/Co/Zn/Cd) efflux pump
                     [Inorganic ion transport and metabolism]; COG0053"
                     /db_xref="CDD:439823"
     CDS             1..243
                     /gene="LOC144113211"
                     /coded_by="XM_077646177.1:308..1039"
                     /db_xref="GeneID:144113211"
ORIGIN      
        1 mernglltsg sngkvrvlne ttplkldsql lengpfeggd lnrkcvltls ivsvvfnivv
       61 ggtgfvfafq rnsasiyafa aeclldmlss miviwqyltp hysfarerva cillglffil
      121 saiaiiskam ddlivrespt wipvliilag vgaaacflla vvklilakrl gslslvldgi
      181 nslissfcav aiivsdlvyd snpevwymda vlstvaalim llvgakvlfe nwsigrhghm
      241 tlq
//
LOCUS       XP_077502304             243 aa            linear   INV 25-NOV-2025
DEFINITION  transmembrane protein 163a-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502304
VERSION     XP_077502304.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646178.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..243
                     /product="transmembrane protein 163a-like isoform X2"
                     /calculated_mol_wt=26197
     Region          50..>233
                     /region_name="FieF"
                     /note="Divalent metal cation (Fe/Co/Zn/Cd) efflux pump
                     [Inorganic ion transport and metabolism]; COG0053"
                     /db_xref="CDD:439823"
     CDS             1..243
                     /gene="LOC144113211"
                     /coded_by="XM_077646178.1:193..924"
                     /db_xref="GeneID:144113211"
ORIGIN      
        1 mernglltsg sngkvrvlne ttplkldsql lengpfeggd lnrkcvltls ivsvvfnivv
       61 ggtgfvfafq rnsasiyafa aeclldmlss miviwqyltp hysfarerva cillglffil
      121 saiaiiskam ddlivrespt wipvliilag vgaaacflla vvklilakrl gslslvldgi
      181 nslissfcav aiivsdlvyd snpevwymda vlstvaalim llvgakvlfe nwsigrhghm
      241 tlq
//
LOCUS       XP_077502305             239 aa            linear   INV 25-NOV-2025
DEFINITION  transmembrane protein 163a-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077502305
VERSION     XP_077502305.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646179.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..239
                     /product="transmembrane protein 163a-like isoform X3"
                     /calculated_mol_wt=26076
     Region          46..>229
                     /region_name="FieF"
                     /note="Divalent metal cation (Fe/Co/Zn/Cd) efflux pump
                     [Inorganic ion transport and metabolism]; COG0053"
                     /db_xref="CDD:439823"
     CDS             1..239
                     /gene="LOC144113211"
                     /coded_by="XM_077646179.1:284..1003"
                     /db_xref="GeneID:144113211"
ORIGIN      
        1 mgfhswepyh fhyadettpl kldsqlleng pfeggdlnrk cvltlsivsv vfnivvggtg
       61 fvfafqrnsa siyafaaecl ldmlssmivi wqyltphysf arervacill glffilsaia
      121 iiskamddli vresptwipv liilagvgaa acfllavvkl ilakrlgsls lvldginsli
      181 ssfcavaiiv sdlvydsnpe vwymdavlst vaalimllvg akvlfenwsi grhghmtlq
//
LOCUS       XP_077502306             168 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113213 [Amblyomma americanum].
ACCESSION   XP_077502306
VERSION     XP_077502306.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646180.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..168
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..168
                     /product="uncharacterized protein LOC144113213"
                     /calculated_mol_wt=18388
     CDS             1..168
                     /gene="LOC144113213"
                     /coded_by="XM_077646180.1:270..776"
                     /db_xref="GeneID:144113213"
ORIGIN      
        1 mapayavasp gplfavqvaq ratephahap svgtrprmpa drgrcqllps alvalwlstf
       61 acaahaakkd clidkekgwf dpsnvyqcvn sdccteygkh sccakkpkse ivqeqlllwg
      121 gllgflllla viiyckrkdv qifegrslrc kfcpgqrdri enirsnva
//
LOCUS       XP_077502307             907 aa            linear   INV 25-NOV-2025
DEFINITION  dual specificity calcium/calmodulin-dependent 3',5'-cyclic
            nucleotide phosphodiesterase 1-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502307
VERSION     XP_077502307.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646181.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..907
                     /product="dual specificity calcium/calmodulin-dependent
                     3',5'-cyclic nucleotide phosphodiesterase 1-like isoform
                     X1"
                     /calculated_mol_wt=100768
     Region          281..341
                     /region_name="PDEase_I_N"
                     /note="3'5'-cyclic nucleotide phosphodiesterase
                     N-terminal; pfam08499"
                     /db_xref="CDD:462497"
     Region          426..648
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..907
                     /gene="LOC144113214"
                     /coded_by="XM_077646181.1:323..3046"
                     /db_xref="GeneID:144113214"
ORIGIN      
        1 mqidgpgape ssllaqnrmg kdalqksnsv plmtgnsrrp eatrkcvlrl dgysylivan
       61 pppeapakda pegeaeeeda gdptkpgtvv vrrfqprvat vvrekageqc deggrqndaa
      121 aeteasaagd rrhgkshgal gdarplppvd tleacdkaan rlrdlsthlq hgdvpldmlq
      181 ktlqyaacvl eavcqdetrk ltpippqppp slletplpps gvatsmsvcp nekripatdv
      241 lpadsvprqp gveqrrrali nanskfnsgs fskgaqsffr kmldeeddls evqpdavpne
      301 vrewlastft rqaatqrkrs edkprfrsva nairagimve kiyrrmssst llqipphlan
      361 llkyvddwcf dvfnvnevan gqvlryltye lfgrygivhk fkissaalen fisaveqgyc
      421 kyknpyhnnl haadvtqtvh ymlysagiss wlteleafat lfaaiihdye htgttnnfhv
      481 msrsetaliy ndrsvlenhh isaafrllte ddqnilssls keeykefrll viemvlatdm
      541 tshfqqvktm ktalshhdfs ldkakalsli lhccdishps kewrlhhrwt yllmeeffqq
      601 gdkehelglp ysplcdrntt liaesqigfi dfilnptmdv cgdllekill qvaqraneds
      661 iseegsenki tevlseddgk aqddskakdd akqqddskqe dakphedgkk lsdektqqrs
      721 lstvrpmrtt sttsitslts itaistdegi ksgkvgsaps sprspkgqla pkelkrpwlk
      781 yleenrsmwq eraaqdaear rkraspgneg lessrtestp aesktvqlkp latkpseskl
      841 aelqplqsks lesmplehkp lesrtpesrl leskhtdpht mdskklelss lestpleata
      901 dtttsah
//
LOCUS       XP_077502308             896 aa            linear   INV 25-NOV-2025
DEFINITION  dual specificity calcium/calmodulin-dependent 3',5'-cyclic
            nucleotide phosphodiesterase 1-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502308
VERSION     XP_077502308.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646182.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..896
                     /product="dual specificity calcium/calmodulin-dependent
                     3',5'-cyclic nucleotide phosphodiesterase 1-like isoform
                     X2"
                     /calculated_mol_wt=99801
     Region          270..330
                     /region_name="PDEase_I_N"
                     /note="3'5'-cyclic nucleotide phosphodiesterase
                     N-terminal; pfam08499"
                     /db_xref="CDD:462497"
     Region          415..637
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..896
                     /gene="LOC144113214"
                     /coded_by="XM_077646182.1:36..2726"
                     /db_xref="GeneID:144113214"
ORIGIN      
        1 mqrrnqrlra fvcvcvcarv fcvsgvcirg geylklpgna dhfehpmanp ppeapakdap
       61 egeaeeedag dptkpgtvvv rrfqprvatv vrekageqcd eggrqndaaa eteasaagdr
      121 rhgkshgalg darplppvdt leacdkaanr lrdlsthlqh gdvpldmlqk tlqyaacvle
      181 avcqdetrkl tpippqppps lletplppsg vatsmsvcpn ekripatdvl padsvprqpg
      241 veqrrralin anskfnsgsf skgaqsffrk mldeeddlse vqpdavpnev rewlastftr
      301 qaatqrkrse dkprfrsvan airagimvek iyrrmssstl lqipphlanl lkyvddwcfd
      361 vfnvnevang qvlryltyel fgrygivhkf kissaalenf isaveqgyck yknpyhnnlh
      421 aadvtqtvhy mlysagissw lteleafatl faaiihdyeh tgttnnfhvm srsetaliyn
      481 drsvlenhhi saafrllted dqnilsslsk eeykefrllv iemvlatdmt shfqqvktmk
      541 talshhdfsl dkakalslil hccdishpsk ewrlhhrwty llmeeffqqg dkehelglpy
      601 splcdrnttl iaesqigfid filnptmdvc gdllekillq vaqranedsi seegsenkit
      661 evlseddgka qddskakdda kqqddskqed akphedgkkl sdektqqrsl stvrpmrtts
      721 ttsitsltsi taistdegik sgkvgsapss prspkgqlap kelkrpwlky leenrsmwqe
      781 raaqdaearr kraspgnegl essrtestpa esktvqlkpl atkpseskla elqplqsksl
      841 esmplehkpl esrtpesrll eskhtdphtm dskklelssl estpleatad tttsah
//
LOCUS       XP_077502309             889 aa            linear   INV 25-NOV-2025
DEFINITION  dual specificity calcium/calmodulin-dependent 3',5'-cyclic
            nucleotide phosphodiesterase 1-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502309
VERSION     XP_077502309.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646183.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..889
                     /product="dual specificity calcium/calmodulin-dependent
                     3',5'-cyclic nucleotide phosphodiesterase 1-like isoform
                     X3"
                     /calculated_mol_wt=98902
     Region          263..323
                     /region_name="PDEase_I_N"
                     /note="3'5'-cyclic nucleotide phosphodiesterase
                     N-terminal; pfam08499"
                     /db_xref="CDD:462497"
     Region          408..630
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..889
                     /gene="LOC144113214"
                     /coded_by="XM_077646183.1:306..2975"
                     /db_xref="GeneID:144113214"
ORIGIN      
        1 mgkdalqksn svplmtgnsr rpeatrkcvl rldgysyliv anpppeapak dapegeaeee
       61 dagdptkpgt vvvrrfqprv atvvrekage qcdeggrqnd aaaeteasaa gdrrhgkshg
      121 algdarplpp vdtleacdka anrlrdlsth lqhgdvpldm lqktlqyaac vleavcqdet
      181 rkltpippqp ppslletplp psgvatsmsv cpnekripat dvlpadsvpr qpgveqrrra
      241 linanskfns gsfskgaqsf frkmldeedd lsevqpdavp nevrewlast ftrqaatqrk
      301 rsedkprfrs vanairagim vekiyrrmss stllqipphl anllkyvddw cfdvfnvnev
      361 angqvlrylt yelfgrygiv hkfkissaal enfisaveqg yckyknpyhn nlhaadvtqt
      421 vhymlysagi sswlteleaf atlfaaiihd yehtgttnnf hvmsrsetal iyndrsvlen
      481 hhisaafrll teddqnilss lskeeykefr llviemvlat dmtshfqqvk tmktalshhd
      541 fsldkakals lilhccdish pskewrlhhr wtyllmeeff qqgdkehelg lpysplcdrn
      601 ttliaesqig fidfilnptm dvcgdlleki llqvaqrane dsiseegsen kitevlsedd
      661 gkaqddskak ddakqqddsk qedakphedg kklsdektqq rslstvrpmr ttsttsitsl
      721 tsitaistde giksgkvgsa pssprspkgq lapkelkrpw lkyleenrsm wqeraaqdae
      781 arrkraspgn eglessrtes tpaesktvql kplatkpses klaelqplqs kslesmpleh
      841 kplesrtpes rlleskhtdp htmdskklel sslestplea tadtttsah
//
LOCUS       XP_077502310             888 aa            linear   INV 25-NOV-2025
DEFINITION  dual specificity calcium/calmodulin-dependent 3',5'-cyclic
            nucleotide phosphodiesterase 1-like isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077502310
VERSION     XP_077502310.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646184.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..888
                     /product="dual specificity calcium/calmodulin-dependent
                     3',5'-cyclic nucleotide phosphodiesterase 1-like isoform
                     X4"
                     /calculated_mol_wt=98566
     Region          262..322
                     /region_name="PDEase_I_N"
                     /note="3'5'-cyclic nucleotide phosphodiesterase
                     N-terminal; pfam08499"
                     /db_xref="CDD:462497"
     Region          407..629
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..888
                     /gene="LOC144113214"
                     /coded_by="XM_077646184.1:16..2682"
                     /db_xref="GeneID:144113214"
ORIGIN      
        1 mqaagatagv asssaepaqh pksrrrwnkt raakkaqqva npppeapakd apegeaeeed
       61 agdptkpgtv vvrrfqprva tvvrekageq cdeggrqnda aaeteasaag drrhgkshga
      121 lgdarplppv dtleacdkaa nrlrdlsthl qhgdvpldml qktlqyaacv leavcqdetr
      181 kltpippqpp pslletplpp sgvatsmsvc pnekripatd vlpadsvprq pgveqrrral
      241 inanskfnsg sfskgaqsff rkmldeeddl sevqpdavpn evrewlastf trqaatqrkr
      301 sedkprfrsv anairagimv ekiyrrmsss tllqipphla nllkyvddwc fdvfnvneva
      361 ngqvlrylty elfgrygivh kfkissaale nfisaveqgy ckyknpyhnn lhaadvtqtv
      421 hymlysagis swlteleafa tlfaaiihdy ehtgttnnfh vmsrsetali yndrsvlenh
      481 hisaafrllt eddqnilssl skeeykefrl lviemvlatd mtshfqqvkt mktalshhdf
      541 sldkakalsl ilhccdishp skewrlhhrw tyllmeeffq qgdkehelgl pysplcdrnt
      601 tliaesqigf idfilnptmd vcgdllekil lqvaqraned siseegsenk itevlseddg
      661 kaqddskakd dakqqddskq edakphedgk klsdektqqr slstvrpmrt tsttsitslt
      721 sitaistdeg iksgkvgsap ssprspkgql apkelkrpwl kyleenrsmw qeraaqdaea
      781 rrkraspgne glessrtest paesktvqlk platkpsesk laelqplqsk slesmplehk
      841 plesrtpesr lleskhtdph tmdskklels slestpleat adtttsah
//
LOCUS       XP_077502311             877 aa            linear   INV 25-NOV-2025
DEFINITION  dual specificity calcium/calmodulin-dependent 3',5'-cyclic
            nucleotide phosphodiesterase 1-like isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077502311
VERSION     XP_077502311.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646185.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..877
                     /product="dual specificity calcium/calmodulin-dependent
                     3',5'-cyclic nucleotide phosphodiesterase 1-like isoform
                     X5"
                     /calculated_mol_wt=97675
     Region          251..311
                     /region_name="PDEase_I_N"
                     /note="3'5'-cyclic nucleotide phosphodiesterase
                     N-terminal; pfam08499"
                     /db_xref="CDD:462497"
     Region          396..618
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..877
                     /gene="LOC144113214"
                     /coded_by="XM_077646185.1:622..3255"
                     /db_xref="GeneID:144113214"
ORIGIN      
        1 mslnkdprhf kkcksatfrv dglsytivan pppeapakda pegeaeeeda gdptkpgtvv
       61 vrrfqprvat vvrekageqc deggrqndaa aeteasaagd rrhgkshgal gdarplppvd
      121 tleacdkaan rlrdlsthlq hgdvpldmlq ktlqyaacvl eavcqdetrk ltpippqppp
      181 slletplpps gvatsmsvcp nekripatdv lpadsvprqp gveqrrrali nanskfnsgs
      241 fskgaqsffr kmldeeddls evqpdavpne vrewlastft rqaatqrkrs edkprfrsva
      301 nairagimve kiyrrmssst llqipphlan llkyvddwcf dvfnvnevan gqvlryltye
      361 lfgrygivhk fkissaalen fisaveqgyc kyknpyhnnl haadvtqtvh ymlysagiss
      421 wlteleafat lfaaiihdye htgttnnfhv msrsetaliy ndrsvlenhh isaafrllte
      481 ddqnilssls keeykefrll viemvlatdm tshfqqvktm ktalshhdfs ldkakalsli
      541 lhccdishps kewrlhhrwt yllmeeffqq gdkehelglp ysplcdrntt liaesqigfi
      601 dfilnptmdv cgdllekill qvaqraneds iseegsenki tevlseddgk aqddskakdd
      661 akqqddskqe dakphedgkk lsdektqqrs lstvrpmrtt sttsitslts itaistdegi
      721 ksgkvgsaps sprspkgqla pkelkrpwlk yleenrsmwq eraaqdaear rkraspgneg
      781 lessrtestp aesktvqlkp latkpseskl aelqplqsks lesmplehkp lesrtpesrl
      841 leskhtdpht mdskklelss lestpleata dtttsah
//
LOCUS       XP_077502312             838 aa            linear   INV 25-NOV-2025
DEFINITION  dual specificity calcium/calmodulin-dependent 3',5'-cyclic
            nucleotide phosphodiesterase 1A-like isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077502312
VERSION     XP_077502312.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646186.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..838
                     /product="dual specificity calcium/calmodulin-dependent
                     3',5'-cyclic nucleotide phosphodiesterase 1A-like isoform
                     X6"
                     /calculated_mol_wt=93336
     Region          212..272
                     /region_name="PDEase_I_N"
                     /note="3'5'-cyclic nucleotide phosphodiesterase
                     N-terminal; pfam08499"
                     /db_xref="CDD:462497"
     Region          357..579
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..838
                     /gene="LOC144113214"
                     /coded_by="XM_077646186.1:321..2837"
                     /db_xref="GeneID:144113214"
ORIGIN      
        1 mqidgpgape ssllaqnrmg kdalqksnsv plmtgnsrrp eatrkcvlrl dgysylivan
       61 pppeapakda pegeaeeeda gdptkpgtvv vrrfqprvat vvrekageqc deggrqndaa
      121 aeteasaagd rrhgkshgal gdarplppvd tleacdkaan rlrdlsthlq hgdvpldmlq
      181 ktlqyaacvl eavcqdetsg sfskgaqsff rkmldeeddl sevqpdavpn evrewlastf
      241 trqaatqrkr sedkprfrsv anairagimv ekiyrrmsss tllqipphla nllkyvddwc
      301 fdvfnvneva ngqvlrylty elfgrygivh kfkissaale nfisaveqgy ckyknpyhnn
      361 lhaadvtqtv hymlysagis swlteleafa tlfaaiihdy ehtgttnnfh vmsrsetali
      421 yndrsvlenh hisaafrllt eddqnilssl skeeykefrl lviemvlatd mtshfqqvkt
      481 mktalshhdf sldkakalsl ilhccdishp skewrlhhrw tyllmeeffq qgdkehelgl
      541 pysplcdrnt tliaesqigf idfilnptmd vcgdllekil lqvaqraned siseegsenk
      601 itevlseddg kaqddskakd dakqqddskq edakphedgk klsdektqqr slstvrpmrt
      661 tsttsitslt sitaistdeg iksgkvgsap ssprspkgql apkelkrpwl kyleenrsmw
      721 qeraaqdaea rrkraspgne glessrtest paesktvqlk platkpsesk laelqplqsk
      781 slesmplehk plesrtpesr lleskhtdph tmdskklels slestpleat adtttsah
//
LOCUS       XP_077502313             826 aa            linear   INV 25-NOV-2025
DEFINITION  dual specificity calcium/calmodulin-dependent 3',5'-cyclic
            nucleotide phosphodiesterase 1A-like isoform X7 [Amblyomma
            americanum].
ACCESSION   XP_077502313
VERSION     XP_077502313.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646187.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..826
                     /product="dual specificity calcium/calmodulin-dependent
                     3',5'-cyclic nucleotide phosphodiesterase 1A-like isoform
                     X7"
                     /calculated_mol_wt=92105
     Region          200..260
                     /region_name="PDEase_I_N"
                     /note="3'5'-cyclic nucleotide phosphodiesterase
                     N-terminal; pfam08499"
                     /db_xref="CDD:462497"
     Region          345..567
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..826
                     /gene="LOC144113214"
                     /coded_by="XM_077646187.1:321..2801"
                     /db_xref="GeneID:144113214"
ORIGIN      
        1 mqidgpgape ssllaqnrmg kdalqksnsv plmtgnsrrp eatrkcvlrl dgysylivan
       61 pppeapakda pegeaeeeda gdptkpgtvv vrrfqprvat vvrekageqc deggrqndaa
      121 aeteasaagd rrhgkshgal gdarplppvd tleacdkaan rlrdlsthlq hgdvpldmlq
      181 ktlqyaacvl eavcqdetrk mldeeddlse vqpdavpnev rewlastftr qaatqrkrse
      241 dkprfrsvan airagimvek iyrrmssstl lqipphlanl lkyvddwcfd vfnvnevang
      301 qvlryltyel fgrygivhkf kissaalenf isaveqgyck yknpyhnnlh aadvtqtvhy
      361 mlysagissw lteleafatl faaiihdyeh tgttnnfhvm srsetaliyn drsvlenhhi
      421 saafrllted dqnilsslsk eeykefrllv iemvlatdmt shfqqvktmk talshhdfsl
      481 dkakalslil hccdishpsk ewrlhhrwty llmeeffqqg dkehelglpy splcdrnttl
      541 iaesqigfid filnptmdvc gdllekillq vaqranedsi seegsenkit evlseddgka
      601 qddskakdda kqqddskqed akphedgkkl sdektqqrsl stvrpmrtts ttsitsltsi
      661 taistdegik sgkvgsapss prspkgqlap kelkrpwlky leenrsmwqe raaqdaearr
      721 kraspgnegl essrtestpa esktvqlkpl atkpseskla elqplqsksl esmplehkpl
      781 esrtpesrll eskhtdphtm dskklelssl estpleatad tttsah
//
LOCUS       XP_077502314             820 aa            linear   INV 25-NOV-2025
DEFINITION  dual specificity calcium/calmodulin-dependent 3',5'-cyclic
            nucleotide phosphodiesterase 1A-like isoform X8 [Amblyomma
            americanum].
ACCESSION   XP_077502314
VERSION     XP_077502314.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646188.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..820
                     /product="dual specificity calcium/calmodulin-dependent
                     3',5'-cyclic nucleotide phosphodiesterase 1A-like isoform
                     X8"
                     /calculated_mol_wt=91470
     Region          194..254
                     /region_name="PDEase_I_N"
                     /note="3'5'-cyclic nucleotide phosphodiesterase
                     N-terminal; pfam08499"
                     /db_xref="CDD:462497"
     Region          339..561
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..820
                     /gene="LOC144113214"
                     /coded_by="XM_077646188.1:304..2766"
                     /db_xref="GeneID:144113214"
ORIGIN      
        1 mgkdalqksn svplmtgnsr rpeatrkcvl rldgysyliv anpppeapak dapegeaeee
       61 dagdptkpgt vvvrrfqprv atvvrekage qcdeggrqnd aaaeteasaa gdrrhgkshg
      121 algdarplpp vdtleacdka anrlrdlsth lqhgdvpldm lqktlqyaac vleavcqdet
      181 sgsfskgaqs ffrkmldeed dlsevqpdav pnevrewlas tftrqaatqr krsedkprfr
      241 svanairagi mvekiyrrms sstllqipph lanllkyvdd wcfdvfnvne vangqvlryl
      301 tyelfgrygi vhkfkissaa lenfisaveq gyckyknpyh nnlhaadvtq tvhymlysag
      361 isswltelea fatlfaaiih dyehtgttnn fhvmsrseta liyndrsvle nhhisaafrl
      421 lteddqnils slskeeykef rllviemvla tdmtshfqqv ktmktalshh dfsldkakal
      481 slilhccdis hpskewrlhh rwtyllmeef fqqgdkehel glpysplcdr nttliaesqi
      541 gfidfilnpt mdvcgdllek illqvaqran edsiseegse nkitevlsed dgkaqddska
      601 kddakqqdds kqedakphed gkklsdektq qrslstvrpm rttsttsits ltsitaistd
      661 egiksgkvgs apssprspkg qlapkelkrp wlkyleenrs mwqeraaqda earrkraspg
      721 neglessrte stpaesktvq lkplatkpse sklaelqplq skslesmple hkplesrtpe
      781 srlleskhtd phtmdskkle lsslestple atadtttsah
//
LOCUS       XP_077502315             808 aa            linear   INV 25-NOV-2025
DEFINITION  dual specificity calcium/calmodulin-dependent 3',5'-cyclic
            nucleotide phosphodiesterase 1A-like isoform X9 [Amblyomma
            americanum].
ACCESSION   XP_077502315
VERSION     XP_077502315.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646189.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..808
                     /product="dual specificity calcium/calmodulin-dependent
                     3',5'-cyclic nucleotide phosphodiesterase 1A-like isoform
                     X9"
                     /calculated_mol_wt=90243
     Region          182..242
                     /region_name="PDEase_I_N"
                     /note="3'5'-cyclic nucleotide phosphodiesterase
                     N-terminal; pfam08499"
                     /db_xref="CDD:462497"
     Region          327..549
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..808
                     /gene="LOC144113214"
                     /coded_by="XM_077646189.1:609..3035"
                     /db_xref="GeneID:144113214"
ORIGIN      
        1 mslnkdprhf kkcksatfrv dglsytivan pppeapakda pegeaeeeda gdptkpgtvv
       61 vrrfqprvat vvrekageqc deggrqndaa aeteasaagd rrhgkshgal gdarplppvd
      121 tleacdkaan rlrdlsthlq hgdvpldmlq ktlqyaacvl eavcqdetsg sfskgaqsff
      181 rkmldeeddl sevqpdavpn evrewlastf trqaatqrkr sedkprfrsv anairagimv
      241 ekiyrrmsss tllqipphla nllkyvddwc fdvfnvneva ngqvlrylty elfgrygivh
      301 kfkissaale nfisaveqgy ckyknpyhnn lhaadvtqtv hymlysagis swlteleafa
      361 tlfaaiihdy ehtgttnnfh vmsrsetali yndrsvlenh hisaafrllt eddqnilssl
      421 skeeykefrl lviemvlatd mtshfqqvkt mktalshhdf sldkakalsl ilhccdishp
      481 skewrlhhrw tyllmeeffq qgdkehelgl pysplcdrnt tliaesqigf idfilnptmd
      541 vcgdllekil lqvaqraned siseegsenk itevlseddg kaqddskakd dakqqddskq
      601 edakphedgk klsdektqqr slstvrpmrt tsttsitslt sitaistdeg iksgkvgsap
      661 ssprspkgql apkelkrpwl kyleenrsmw qeraaqdaea rrkraspgne glessrtest
      721 paesktvqlk platkpsesk laelqplqsk slesmplehk plesrtpesr lleskhtdph
      781 tmdskklels slestpleat adtttsah
//
LOCUS       XP_077502316             796 aa            linear   INV 25-NOV-2025
DEFINITION  dual specificity calcium/calmodulin-dependent 3',5'-cyclic
            nucleotide phosphodiesterase 1A-like isoform X10 [Amblyomma
            americanum].
ACCESSION   XP_077502316
VERSION     XP_077502316.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646190.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..796
                     /product="dual specificity calcium/calmodulin-dependent
                     3',5'-cyclic nucleotide phosphodiesterase 1A-like isoform
                     X10"
                     /calculated_mol_wt=89012
     Region          170..230
                     /region_name="PDEase_I_N"
                     /note="3'5'-cyclic nucleotide phosphodiesterase
                     N-terminal; pfam08499"
                     /db_xref="CDD:462497"
     Region          315..537
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..796
                     /gene="LOC144113214"
                     /coded_by="XM_077646190.1:608..2998"
                     /db_xref="GeneID:144113214"
ORIGIN      
        1 mslnkdprhf kkcksatfrv dglsytivan pppeapakda pegeaeeeda gdptkpgtvv
       61 vrrfqprvat vvrekageqc deggrqndaa aeteasaagd rrhgkshgal gdarplppvd
      121 tleacdkaan rlrdlsthlq hgdvpldmlq ktlqyaacvl eavcqdetrk mldeeddlse
      181 vqpdavpnev rewlastftr qaatqrkrse dkprfrsvan airagimvek iyrrmssstl
      241 lqipphlanl lkyvddwcfd vfnvnevang qvlryltyel fgrygivhkf kissaalenf
      301 isaveqgyck yknpyhnnlh aadvtqtvhy mlysagissw lteleafatl faaiihdyeh
      361 tgttnnfhvm srsetaliyn drsvlenhhi saafrllted dqnilsslsk eeykefrllv
      421 iemvlatdmt shfqqvktmk talshhdfsl dkakalslil hccdishpsk ewrlhhrwty
      481 llmeeffqqg dkehelglpy splcdrnttl iaesqigfid filnptmdvc gdllekillq
      541 vaqranedsi seegsenkit evlseddgka qddskakdda kqqddskqed akphedgkkl
      601 sdektqqrsl stvrpmrtts ttsitsltsi taistdegik sgkvgsapss prspkgqlap
      661 kelkrpwlky leenrsmwqe raaqdaearr kraspgnegl essrtestpa esktvqlkpl
      721 atkpseskla elqplqsksl esmplehkpl esrtpesrll eskhtdphtm dskklelssl
      781 estpleatad tttsah
//
LOCUS       XP_077502317             730 aa            linear   INV 25-NOV-2025
DEFINITION  dual specificity calcium/calmodulin-dependent 3',5'-cyclic
            nucleotide phosphodiesterase 1A-like isoform X11 [Amblyomma
            americanum].
ACCESSION   XP_077502317
VERSION     XP_077502317.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646191.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..730
                     /product="dual specificity calcium/calmodulin-dependent
                     3',5'-cyclic nucleotide phosphodiesterase 1A-like isoform
                     X11"
                     /calculated_mol_wt=81964
     Region          104..164
                     /region_name="PDEase_I_N"
                     /note="3'5'-cyclic nucleotide phosphodiesterase
                     N-terminal; pfam08499"
                     /db_xref="CDD:462497"
     Region          249..471
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..730
                     /gene="LOC144113214"
                     /coded_by="XM_077646191.1:616..2808"
                     /db_xref="GeneID:144113214"
ORIGIN      
        1 mlqktlqyaa cvleavcqde trkltpippq pppslletpl ppsgvatsms vcpnekripa
       61 tdvlpadsvp rqpgveqrrr alinanskfn sgsfskgaqs ffrkmldeed dlsevqpdav
      121 pnevrewlas tftrqaatqr krsedkprfr svanairagi mvekiyrrms sstllqipph
      181 lanllkyvdd wcfdvfnvne vangqvlryl tyelfgrygi vhkfkissaa lenfisaveq
      241 gyckyknpyh nnlhaadvtq tvhymlysag isswltelea fatlfaaiih dyehtgttnn
      301 fhvmsrseta liyndrsvle nhhisaafrl lteddqnils slskeeykef rllviemvla
      361 tdmtshfqqv ktmktalshh dfsldkakal slilhccdis hpskewrlhh rwtyllmeef
      421 fqqgdkehel glpysplcdr nttliaesqi gfidfilnpt mdvcgdllek illqvaqran
      481 edsiseegse nkitevlsed dgkaqddska kddakqqdds kqedakphed gkklsdektq
      541 qrslstvrpm rttsttsits ltsitaistd egiksgkvgs apssprspkg qlapkelkrp
      601 wlkyleenrs mwqeraaqda earrkraspg neglessrte stpaesktvq lkplatkpse
      661 sklaelqplq skslesmple hkplesrtpe srlleskhtd phtmdskkle lsslestple
      721 atadtttsah
//
LOCUS       XP_077502318            1389 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113216 [Amblyomma americanum].
ACCESSION   XP_077502318
VERSION     XP_077502318.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646192.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1389
                     /product="uncharacterized protein LOC144113216"
                     /calculated_mol_wt=148238
     Region          <698..1081
                     /region_name="PRK12323"
                     /note="DNA polymerase III subunit gamma/tau; cl46901"
                     /db_xref="CDD:481241"
     Region          924..>1294
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..1389
                     /gene="LOC144113216"
                     /coded_by="XM_077646192.1:437..4606"
                     /db_xref="GeneID:144113216"
ORIGIN      
        1 mdsagepsma chqvseaepv vqeeaqvigy fdlensglql vegdntfylp pealrehvlm
       61 aeegkegqni ivvlggsedd vvqafaense tllgdqatft vnqlenaisa tlpqefagve
      121 avtttegqql lilsedtsek mvslesapve esaavletql lyqedpkdnl pgecteagts
      181 vqseqqpgve kspevngkeg invlpgecte agtsvqserq pgvekspevn gkeginvipe
      241 dsrdktgvvy psqensefqk esrpvplats plgvrdpsdg adsfrisisl aqcgslgqrk
      301 ivvspgakra ggteekqngt vggdivhvkd khsshksvss aqareaaidv dcnsskippa
      361 ssavrrrldl anvllpndlt mekgtaefav qkdigstseg tealdlvrap qntsehvsad
      421 pptacipesk sdepaqvvae gttaesqeaa ekglgaqlfk rlndrdaakm takaatstcs
      481 dgklpvhrea alnrgtvasg teghekcsfr dedsglgsal kqnsleekea krhttaseds
      541 kqqckqfhge hlpgvkdeka ahvppekcsr trtyvdatte evslpsvtat pnsslegeek
      601 pkgsviksav tqkelcqtsh svdaddfees dgegtppvke khgensevdv seeqeeksqe
      661 vteeveneqn vdiansninm edeqevsqql pvgktpsekp staaedntek sldfqtkpgr
      721 geaqneppsd mkmsrevpnk qdgtvcdpat sslaaskfed sptsnshevt qsnvsdhqea
      781 srdigvanal kskvdlaeeq nathspislh sdsdeklrae tetrnidtvc dkaasdssds
      841 rereiecehs dqapkvdskg eyqkpnvefq dvhreavenp esshcelqdr kerdngdaid
      901 kgnqnvrvps ppeandlntk eaicaessde kvlaaldlvp sqeaspapss iavsspppek
      961 tnvaaekpgp ssaasgslgk kkssiavssp ppgktnvaae kpgpssaasg slrkkksria
     1021 vsspppektn vaaekpdvaa ekpgpssaas gslrkkksri avsspppekt nvaaekpdva
     1081 aekpgpssaa sgslgkkkrk keqkvecslg kpgpsshpsd aeekkwdgqe gsssgkkrwn
     1141 lrtptkprkr iiipdsdded fesygsedis aettksrsee vfdvllnvfk teqakkaanh
     1201 aepavkkrak rgglssskrk psfpkpfgsg dgavgkegat stmekykake kpsgrkshpv
     1261 dtpsfvtnet papkrarkrr sepaepaepa mpeackksad gddttetpvt gnripsvtdl
     1321 fagdirircq kpsssndkaa tnyhcskcgf rstrmenivr hhkndcshsr slfdwhteps
     1381 kstnsnapt
//
LOCUS       XP_077502319            1389 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113216 [Amblyomma americanum].
ACCESSION   XP_077502319
VERSION     XP_077502319.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646193.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1389
                     /product="uncharacterized protein LOC144113216"
                     /calculated_mol_wt=148238
     Region          <698..1081
                     /region_name="PRK12323"
                     /note="DNA polymerase III subunit gamma/tau; cl46901"
                     /db_xref="CDD:481241"
     Region          924..>1294
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..1389
                     /gene="LOC144113216"
                     /coded_by="XM_077646193.1:304..4473"
                     /db_xref="GeneID:144113216"
ORIGIN      
        1 mdsagepsma chqvseaepv vqeeaqvigy fdlensglql vegdntfylp pealrehvlm
       61 aeegkegqni ivvlggsedd vvqafaense tllgdqatft vnqlenaisa tlpqefagve
      121 avtttegqql lilsedtsek mvslesapve esaavletql lyqedpkdnl pgecteagts
      181 vqseqqpgve kspevngkeg invlpgecte agtsvqserq pgvekspevn gkeginvipe
      241 dsrdktgvvy psqensefqk esrpvplats plgvrdpsdg adsfrisisl aqcgslgqrk
      301 ivvspgakra ggteekqngt vggdivhvkd khsshksvss aqareaaidv dcnsskippa
      361 ssavrrrldl anvllpndlt mekgtaefav qkdigstseg tealdlvrap qntsehvsad
      421 pptacipesk sdepaqvvae gttaesqeaa ekglgaqlfk rlndrdaakm takaatstcs
      481 dgklpvhrea alnrgtvasg teghekcsfr dedsglgsal kqnsleekea krhttaseds
      541 kqqckqfhge hlpgvkdeka ahvppekcsr trtyvdatte evslpsvtat pnsslegeek
      601 pkgsviksav tqkelcqtsh svdaddfees dgegtppvke khgensevdv seeqeeksqe
      661 vteeveneqn vdiansninm edeqevsqql pvgktpsekp staaedntek sldfqtkpgr
      721 geaqneppsd mkmsrevpnk qdgtvcdpat sslaaskfed sptsnshevt qsnvsdhqea
      781 srdigvanal kskvdlaeeq nathspislh sdsdeklrae tetrnidtvc dkaasdssds
      841 rereiecehs dqapkvdskg eyqkpnvefq dvhreavenp esshcelqdr kerdngdaid
      901 kgnqnvrvps ppeandlntk eaicaessde kvlaaldlvp sqeaspapss iavsspppek
      961 tnvaaekpgp ssaasgslgk kkssiavssp ppgktnvaae kpgpssaasg slrkkksria
     1021 vsspppektn vaaekpdvaa ekpgpssaas gslrkkksri avsspppekt nvaaekpdva
     1081 aekpgpssaa sgslgkkkrk keqkvecslg kpgpsshpsd aeekkwdgqe gsssgkkrwn
     1141 lrtptkprkr iiipdsdded fesygsedis aettksrsee vfdvllnvfk teqakkaanh
     1201 aepavkkrak rgglssskrk psfpkpfgsg dgavgkegat stmekykake kpsgrkshpv
     1261 dtpsfvtnet papkrarkrr sepaepaepa mpeackksad gddttetpvt gnripsvtdl
     1321 fagdirircq kpsssndkaa tnyhcskcgf rstrmenivr hhkndcshsr slfdwhteps
     1381 kstnsnapt
//
LOCUS       XP_077502320            1389 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113216 [Amblyomma americanum].
ACCESSION   XP_077502320
VERSION     XP_077502320.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646194.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1389
                     /product="uncharacterized protein LOC144113216"
                     /calculated_mol_wt=148238
     Region          <698..1081
                     /region_name="PRK12323"
                     /note="DNA polymerase III subunit gamma/tau; cl46901"
                     /db_xref="CDD:481241"
     Region          924..>1294
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..1389
                     /gene="LOC144113216"
                     /coded_by="XM_077646194.1:528..4697"
                     /db_xref="GeneID:144113216"
ORIGIN      
        1 mdsagepsma chqvseaepv vqeeaqvigy fdlensglql vegdntfylp pealrehvlm
       61 aeegkegqni ivvlggsedd vvqafaense tllgdqatft vnqlenaisa tlpqefagve
      121 avtttegqql lilsedtsek mvslesapve esaavletql lyqedpkdnl pgecteagts
      181 vqseqqpgve kspevngkeg invlpgecte agtsvqserq pgvekspevn gkeginvipe
      241 dsrdktgvvy psqensefqk esrpvplats plgvrdpsdg adsfrisisl aqcgslgqrk
      301 ivvspgakra ggteekqngt vggdivhvkd khsshksvss aqareaaidv dcnsskippa
      361 ssavrrrldl anvllpndlt mekgtaefav qkdigstseg tealdlvrap qntsehvsad
      421 pptacipesk sdepaqvvae gttaesqeaa ekglgaqlfk rlndrdaakm takaatstcs
      481 dgklpvhrea alnrgtvasg teghekcsfr dedsglgsal kqnsleekea krhttaseds
      541 kqqckqfhge hlpgvkdeka ahvppekcsr trtyvdatte evslpsvtat pnsslegeek
      601 pkgsviksav tqkelcqtsh svdaddfees dgegtppvke khgensevdv seeqeeksqe
      661 vteeveneqn vdiansninm edeqevsqql pvgktpsekp staaedntek sldfqtkpgr
      721 geaqneppsd mkmsrevpnk qdgtvcdpat sslaaskfed sptsnshevt qsnvsdhqea
      781 srdigvanal kskvdlaeeq nathspislh sdsdeklrae tetrnidtvc dkaasdssds
      841 rereiecehs dqapkvdskg eyqkpnvefq dvhreavenp esshcelqdr kerdngdaid
      901 kgnqnvrvps ppeandlntk eaicaessde kvlaaldlvp sqeaspapss iavsspppek
      961 tnvaaekpgp ssaasgslgk kkssiavssp ppgktnvaae kpgpssaasg slrkkksria
     1021 vsspppektn vaaekpdvaa ekpgpssaas gslrkkksri avsspppekt nvaaekpdva
     1081 aekpgpssaa sgslgkkkrk keqkvecslg kpgpsshpsd aeekkwdgqe gsssgkkrwn
     1141 lrtptkprkr iiipdsdded fesygsedis aettksrsee vfdvllnvfk teqakkaanh
     1201 aepavkkrak rgglssskrk psfpkpfgsg dgavgkegat stmekykake kpsgrkshpv
     1261 dtpsfvtnet papkrarkrr sepaepaepa mpeackksad gddttetpvt gnripsvtdl
     1321 fagdirircq kpsssndkaa tnyhcskcgf rstrmenivr hhkndcshsr slfdwhteps
     1381 kstnsnapt
//
LOCUS       XP_077502321            1389 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113216 [Amblyomma americanum].
ACCESSION   XP_077502321
VERSION     XP_077502321.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646195.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1389
                     /product="uncharacterized protein LOC144113216"
                     /calculated_mol_wt=148238
     Region          <698..1081
                     /region_name="PRK12323"
                     /note="DNA polymerase III subunit gamma/tau; cl46901"
                     /db_xref="CDD:481241"
     Region          924..>1294
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..1389
                     /gene="LOC144113216"
                     /coded_by="XM_077646195.1:434..4603"
                     /db_xref="GeneID:144113216"
ORIGIN      
        1 mdsagepsma chqvseaepv vqeeaqvigy fdlensglql vegdntfylp pealrehvlm
       61 aeegkegqni ivvlggsedd vvqafaense tllgdqatft vnqlenaisa tlpqefagve
      121 avtttegqql lilsedtsek mvslesapve esaavletql lyqedpkdnl pgecteagts
      181 vqseqqpgve kspevngkeg invlpgecte agtsvqserq pgvekspevn gkeginvipe
      241 dsrdktgvvy psqensefqk esrpvplats plgvrdpsdg adsfrisisl aqcgslgqrk
      301 ivvspgakra ggteekqngt vggdivhvkd khsshksvss aqareaaidv dcnsskippa
      361 ssavrrrldl anvllpndlt mekgtaefav qkdigstseg tealdlvrap qntsehvsad
      421 pptacipesk sdepaqvvae gttaesqeaa ekglgaqlfk rlndrdaakm takaatstcs
      481 dgklpvhrea alnrgtvasg teghekcsfr dedsglgsal kqnsleekea krhttaseds
      541 kqqckqfhge hlpgvkdeka ahvppekcsr trtyvdatte evslpsvtat pnsslegeek
      601 pkgsviksav tqkelcqtsh svdaddfees dgegtppvke khgensevdv seeqeeksqe
      661 vteeveneqn vdiansninm edeqevsqql pvgktpsekp staaedntek sldfqtkpgr
      721 geaqneppsd mkmsrevpnk qdgtvcdpat sslaaskfed sptsnshevt qsnvsdhqea
      781 srdigvanal kskvdlaeeq nathspislh sdsdeklrae tetrnidtvc dkaasdssds
      841 rereiecehs dqapkvdskg eyqkpnvefq dvhreavenp esshcelqdr kerdngdaid
      901 kgnqnvrvps ppeandlntk eaicaessde kvlaaldlvp sqeaspapss iavsspppek
      961 tnvaaekpgp ssaasgslgk kkssiavssp ppgktnvaae kpgpssaasg slrkkksria
     1021 vsspppektn vaaekpdvaa ekpgpssaas gslrkkksri avsspppekt nvaaekpdva
     1081 aekpgpssaa sgslgkkkrk keqkvecslg kpgpsshpsd aeekkwdgqe gsssgkkrwn
     1141 lrtptkprkr iiipdsdded fesygsedis aettksrsee vfdvllnvfk teqakkaanh
     1201 aepavkkrak rgglssskrk psfpkpfgsg dgavgkegat stmekykake kpsgrkshpv
     1261 dtpsfvtnet papkrarkrr sepaepaepa mpeackksad gddttetpvt gnripsvtdl
     1321 fagdirircq kpsssndkaa tnyhcskcgf rstrmenivr hhkndcshsr slfdwhteps
     1381 kstnsnapt
//
LOCUS       XP_077502322             360 aa            linear   INV 25-NOV-2025
DEFINITION  elongator complex protein 4 [Amblyomma americanum].
ACCESSION   XP_077502322
VERSION     XP_077502322.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646196.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..360
                     /product="elongator complex protein 4"
                     /calculated_mol_wt=38973
     Region          16..331
                     /region_name="PAXNEB"
                     /note="PAXNEB protein; pfam05625"
                     /db_xref="CDD:461696"
     CDS             1..360
                     /gene="Elp4"
                     /coded_by="XM_077646196.1:240..1322"
                     /db_xref="GeneID:144113217"
ORIGIN      
        1 msssfqkctr pahrgipgtk lspltaetvl scgvsavdfi lggglplggi llaeedafgt
       61 ysrqllkyyv aegieqkhlv yfaggdveps kflrelpkal qfsaagqpas assttsdgdl
      121 kiafryerma kqdslldyde ldhvfdfsdr iestklygac vntfspcetw gnaadrsagv
      181 vepnyasvlg cvsslvdkak tddsashtav rialqglggp awgsvasvcs flhalkalir
      241 gepasvfvtv pevitkahps vlrccrrvvd ivlkvqafdd herganplyk nyhgllqivk
      301 lcrlsslgvr mpdcdflfhq rskkflverl hlppeletee ttstdklsgl acasnqkfsf
//
LOCUS       XP_077502323             564 aa            linear   INV 25-NOV-2025
DEFINITION  frizzled class receptor [Amblyomma americanum].
ACCESSION   XP_077502323
VERSION     XP_077502323.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646197.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..564
                     /product="frizzled class receptor"
                     /calculated_mol_wt=64420
     Region          39..156
                     /region_name="CRD_FZ1_like"
                     /note="Cysteine-rich Wnt-binding domain (CRD) of receptors
                     similar to frizzled 1; cd07458"
                     /db_xref="CDD:143567"
     Site            order(48,50..52,54..56)
                     /site_type="other"
                     /note="putative Wnt binding site [polypeptide binding]"
                     /db_xref="CDD:143567"
     Region          213..548
                     /region_name="7tmF_FZD1_insect"
                     /note="class F insect frizzled subfamily 1, member of
                     7-transmembrane G protein-coupled receptors; cd15248"
                     /db_xref="CDD:320376"
     Site            order(214,223,273,300,382,387,389,392..393,398,473,
                     476..477,509,514,517..518)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:320376"
     Region          222..247
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320376"
     Region          256..277
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320376"
     Region          314..340
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320376"
     Region          357..373
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320376"
     Region          395..424
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320376"
     Region          444..471
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320376"
     Region          507..532
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320376"
     CDS             1..564
                     /gene="fz"
                     /coded_by="XM_077646197.1:309..2003"
                     /db_xref="GeneID:144113223"
ORIGIN      
        1 mrprnvalgl valaallapg lgqvvssrvt ehrtiphhgr ceaitiplck diqynetimp
       61 nllnhqkqed agmevhqffp lvkvkcspdl qfflcsmyap vctileypip pcrslcmsar
      121 egcehlmnkf gfkwpeslec dkfpevgsek lcvgenvteh rinhapsspn stgplvsrgf
      181 lcprefrvpl gldyvfrirg keekncgmpc hhmffrgenl kfsrywigaw aiqcmvstlf
      241 tvltflidmq rfryperpii flsfcnlmvs styfagfvlg dqvacnepfe vpggqsniem
      301 vrtitqnnkk eactvmfmvl yffsmagsiw wvvltltwfl aaglkwghea iesnsqyfhl
      361 aawtipavkt itilamgkve gdvlsgvcyv glwnmdalrg fvlaplfvyl algkvflmag
      421 fislchirtv mkhdgtktdk leklmvrigi fsflytlpal tvlccyfyeq ahfddwmlaw
      481 hwqvntekrw sipcpepscn rrdrslrrpe fyvfmikylg ilivgitsgv wiwsgktfas
      541 wrnfyarlqn ltstparrra eayv
//
LOCUS       XP_077502324             229 aa            linear   INV 25-NOV-2025
DEFINITION  leukocyte surface antigen CD53-like [Amblyomma americanum].
ACCESSION   XP_077502324
VERSION     XP_077502324.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646198.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..229
                     /product="leukocyte surface antigen CD53-like"
                     /calculated_mol_wt=24659
     Region          24..226
                     /region_name="Tetraspanin"
                     /note="Tetraspanin family; pfam00335"
                     /db_xref="CDD:459767"
     CDS             1..229
                     /gene="LOC144113224"
                     /coded_by="XM_077646198.1:126..815"
                     /db_xref="GeneID:144113224"
ORIGIN      
        1 mvqgcgmsci knllfifnff faisgiviiv cggyslhlfk ktgpvigddy vsapvilivv
       61 gsivflvafl gccgamqesy cmlmlfsvfl flilvaeiaa galgfvykgk vnniakdrft
      121 dtlkdykres egkvkpvqea wdfiqqqlkc cgvtgpgdwq tyaqmdppqs ccssgtgtgn
      181 citftkgcyd kvkedisqya vyvgiagigi glieiigiif scclanqvk
//
LOCUS       XP_077502325             115 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113225 [Amblyomma americanum].
ACCESSION   XP_077502325
VERSION     XP_077502325.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646199.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..115
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..115
                     /product="uncharacterized protein LOC144113225"
                     /calculated_mol_wt=12961
     CDS             1..115
                     /gene="LOC144113225"
                     /coded_by="XM_077646199.1:270..617"
                     /db_xref="GeneID:144113225"
ORIGIN      
        1 mrflgcsvsm micklepfsq atqdkhirdt lfdpsltlrk rkkdvsgklp lespmhaplv
       61 sdrqtrptmd kmtkgsgqkr ipedevaadl rillahshra qramcddssd lrvvw
//
LOCUS       XP_077502326             195 aa            linear   INV 25-NOV-2025
DEFINITION  COP9 signalosome complex subunit 8-like [Amblyomma americanum].
ACCESSION   XP_077502326
VERSION     XP_077502326.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646200.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..195
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..195
                     /product="COP9 signalosome complex subunit 8-like"
                     /calculated_mol_wt=21480
     Region          34..166
                     /region_name="CSN8_PSD8_EIF3K"
                     /note="CSN8/PSMD8/EIF3K family; pfam10075"
                     /db_xref="CDD:462954"
     CDS             1..195
                     /gene="LOC144113228"
                     /coded_by="XM_077646200.1:136..723"
                     /db_xref="GeneID:144113228"
ORIGIN      
        1 malvgdgsgd armreyskla adleqqelea adgiaspqty gqllaiyllq ndlpnakflw
       61 kripqeikqs hpelgnmwkv gqalwykdfp aiytglaqew pddikpimqe lgertrrral
      121 tlvakayssi slddasaflg vpkvdlaavv cplgwvvdva sgmvlpkyta arhedstpse
      181 eqlakltdfv aflen
//
LOCUS       XP_077502327             459 aa            linear   INV 25-NOV-2025
DEFINITION  MPN domain-containing protein-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502327
VERSION     XP_077502327.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646201.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..459
                     /product="MPN domain-containing protein-like isoform X1"
                     /calculated_mol_wt=51925
     Region          20..130
                     /region_name="RAMA"
                     /note="Restriction Enzyme Adenine Methylase Associated;
                     pfam18755"
                     /db_xref="CDD:465856"
     Region          205..398
                     /region_name="MPN_2A_DUB"
                     /note="Mov34/MPN/PAD-1 family: Histone H2A deubiquitinase;
                     cd08067"
                     /db_xref="CDD:163698"
     Site            order(231,288,290,298,301)
                     /site_type="active"
                     /note="MPN+ (JAMM) motif [active]"
                     /db_xref="CDD:163698"
     Site            order(288,290,301)
                     /site_type="other"
                     /note="Zinc-binding site [ion binding]"
                     /db_xref="CDD:163698"
     CDS             1..459
                     /gene="LOC144113229"
                     /coded_by="XM_077646201.1:245..1624"
                     /db_xref="GeneID:144113229"
ORIGIN      
        1 mstkiededl dageqsgdee deegnlskrr gahtgrsvtl smlmsdgilq hgenvlsidy
       61 lgsrftgdll kdgkikwret qevysspssw avhckkllnp dkksgcgwst irykgkkldw
      121 ykatwfrkqr ikkddrennv gakvptgekt ksgivikkpl ayqnapeqpv pketlilshg
      181 algtrnvdcd lntlvistpf svldriqpft ltistnclll idfhchltes evvgylggtw
      241 diaahnlsil qafpcrgrlg dkeaapaiee eiresleqrh ltvvgwyhsh pkappqpslr
      301 dcncqldyqi tmkgesdssy tpcvgliccr ctyenqapyv kdescvdaky laywvmpppd
      361 hrpneygrpm qmmynvaqds fltqdllmem rllseyyrgs pdalnfckdf ephnlsywek
      421 lkrsltsklp rdlqvtsgdt qgqavdhfwe fvkglimpv
//
LOCUS       XP_077502328             451 aa            linear   INV 25-NOV-2025
DEFINITION  MPN domain-containing protein-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502328
VERSION     XP_077502328.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646202.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..451
                     /product="MPN domain-containing protein-like isoform X2"
                     /calculated_mol_wt=50943
     Region          20..130
                     /region_name="RAMA"
                     /note="Restriction Enzyme Adenine Methylase Associated;
                     pfam18755"
                     /db_xref="CDD:465856"
     Region          205..390
                     /region_name="MPN_2A_DUB"
                     /note="Mov34/MPN/PAD-1 family: Histone H2A deubiquitinase;
                     cd08067"
                     /db_xref="CDD:163698"
     Site            order(231,288,290,298,301)
                     /site_type="active"
                     /note="MPN+ (JAMM) motif [active]"
                     /db_xref="CDD:163698"
     Site            order(288,290,301)
                     /site_type="other"
                     /note="Zinc-binding site [ion binding]"
                     /db_xref="CDD:163698"
     CDS             1..451
                     /gene="LOC144113229"
                     /coded_by="XM_077646202.1:245..1600"
                     /db_xref="GeneID:144113229"
ORIGIN      
        1 mstkiededl dageqsgdee deegnlskrr gahtgrsvtl smlmsdgilq hgenvlsidy
       61 lgsrftgdll kdgkikwret qevysspssw avhckkllnp dkksgcgwst irykgkkldw
      121 ykatwfrkqr ikkddrennv gakvptgekt ksgivikkpl ayqnapeqpv pketlilshg
      181 algtrnvdcd lntlvistpf svldriqpft ltistnclll idfhchltes evvgylggtw
      241 diaahnlsil qafpcrgrlg dkeaapaiee eiresleqrh ltvvgwyhsh pkappqpslr
      301 dcncqldyqi tmkgesdssy tpcvglicsp yvkdescvda kylaywvmpp pdhrpneygr
      361 pmqmmynvaq dsfltqdllm emrllseyyr gspdalnfck dfephnlsyw eklkrsltsk
      421 lprdlqvtsg dtqgqavdhf wefvkglimp v
//
LOCUS       XP_077502329             303 aa            linear   INV 25-NOV-2025
DEFINITION  Krueppel-like factor 6 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502329
VERSION     XP_077502329.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646203.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..303
                     /product="Krueppel-like factor 6 isoform X1"
                     /calculated_mol_wt=33078
     Region          2..>100
                     /region_name="KLF6_7_N-like"
                     /note="N-terminal domain of Kruppel-like factor (KLF) 6,
                     KLF7, and similar proteins; cd21973"
                     /db_xref="CDD:409246"
     Region          <214..>303
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          225..244
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(229,231,233,235..236,239..240,243,259,261,265..266,
                     269..270,273,287,289,291,293..294,297..298,301)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          252..274
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          282..302
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..303
                     /gene="LOC144113230"
                     /coded_by="XM_077646203.1:487..1398"
                     /db_xref="GeneID:144113230"
ORIGIN      
        1 mdilpsgnif helqaihdtg ffpaqpsled fwqqtiwemd rylkdeprga askrcsgrel
       61 ssstdeaarl wdecsspada kllladsgig ssdlgdssea egdrlsddld fgagamdnas
      121 essassalsw esdgsakaqp eqpfslvqra altppsspea arprggrrha asaepsgtaa
      181 aaaappllta gaaaakqahf nhaarggrrf dvdsskrrih rcqfngckkv ytksshlkah
      241 qrthtgekpy kcswegcewr farsdeltrh yrkhtgskpf kcnhcdrcfs rsdhlalhmk
      301 rhq
//
LOCUS       XP_077502330             271 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113230 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502330
VERSION     XP_077502330.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646204.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..271
                     /product="uncharacterized protein LOC144113230 isoform X2"
                     /calculated_mol_wt=29425
     Region          <2..>68
                     /region_name="KLF6_7_N-like"
                     /note="N-terminal domain of Kruppel-like factor (KLF) 6,
                     KLF7, and similar proteins; cl41732"
                     /db_xref="CDD:425363"
     Region          <182..>271
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          193..212
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(197,199,201,203..204,207..208,211,227,229,233..234,
                     237..238,241,255,257,259,261..262,265..266,269)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          220..242
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          250..270
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..271
                     /gene="LOC144113230"
                     /coded_by="XM_077646204.1:18..833"
                     /db_xref="GeneID:144113230"
ORIGIN      
        1 mqtiwemdry lkdeprgaas krcsgrelss stdeaarlwd ecsspadakl lladsgigss
       61 dlgdsseaeg drlsddldfg agamdnases sassalswes dgsakaqpeq pfslvqraal
      121 tppsspeaar prggrrhaas aepsgtaaaa aapplltaga aaakqahfnh aarggrrfdv
      181 dsskrrihrc qfngckkvyt ksshlkahqr thtgekpykc swegcewrfa rsdeltrhyr
      241 khtgskpfkc nhcdrcfsrs dhlalhmkrh q
//
LOCUS       XP_077502331             265 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113230 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502331
VERSION     XP_077502331.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646205.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..265
                     /product="uncharacterized protein LOC144113230 isoform X3"
                     /calculated_mol_wt=28636
     Region          <171..>265
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          187..206
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(191,193,195,197..198,201..202,205,221,223,227..228,
                     231..232,235,249,251,253,255..256,259..260,263)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          214..236
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          244..264
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..265
                     /gene="LOC144113230"
                     /coded_by="XM_077646205.1:133..930"
                     /db_xref="GeneID:144113230"
ORIGIN      
        1 mdrylkdepr gaaskrcsgr elssstdeaa rlwdecsspa dakllladsg igssdlgdss
       61 eaegdrlsdd ldfgagamdn asessassal swesdgsaka qpeqpfslvq raaltppssp
      121 eaarprggrr haasaepsgt aaaaaappll tagaaaakqa hfnhaarggr rfdvdsskrr
      181 ihrcqfngck kvytksshlk ahqrthtgek pykcswegce wrfarsdelt rhyrkhtgsk
      241 pfkcnhcdrc fsrsdhlalh mkrhq
//
LOCUS       XP_077502332             977 aa            linear   INV 25-NOV-2025
DEFINITION  RAB3 GTPase activating protein subunit 1 [Amblyomma americanum].
ACCESSION   XP_077502332
VERSION     XP_077502332.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646206.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..977
                     /product="RAB3 GTPase activating protein subunit 1"
                     /calculated_mol_wt=109461
     Region          599..752
                     /region_name="Rab3-GTPase_cat"
                     /note="Rab3 GTPase-activating protein catalytic subunit;
                     pfam13890"
                     /db_xref="CDD:464022"
     Region          764..977
                     /region_name="Rab3-GAP_cat_C"
                     /note="Rab3 GTPase-activating protein catalytic subunit
                     C-terminal; pfam19533"
                     /db_xref="CDD:466115"
     CDS             1..977
                     /gene="Rab3GAP1"
                     /coded_by="XM_077646206.1:282..3215"
                     /db_xref="GeneID:144113232"
ORIGIN      
        1 mgddglfded pdlyeitdft tstewerfia sveavihewk ltavrtveal kegelsvqdw
       61 ktcsedikfa sfafkitrhy leespkssee tlnledepap faledmmtlg ndfpprahyl
      121 vrwfglrdfl viapsceeev issedrtkll vssvsvalnn skcslpafvq vhhpqerlcf
      181 gvcvgggltt hfemvhlnhv pkpcsnvsgl lslfkgklsy asqqsptpiq ltirftymlq
      241 dwspaswpql ppdvtndwfd ignlklgklp fgaledpise mqlsvtwpsi pedvvvdndv
      301 ysdlrpenap kwsirmrwkd npeclmeeyl rnffvvcqmt nslddvlknm vqtedlsaka
      361 eildaldrla qpnalpfrlp qlpqpevsse sprnmllrnq ellievlgyi flssdaspqe
      421 knmpaecsem espsethtst kllelkesln slktthpnsl lwrlalaaah vyhacggiea
      481 laqlwvkvia emryywdnnl alpslepgtp nlaasilhqk lqmlnycier kkkrerrhlp
      541 addsgsqrks gssesdedvf yecpddivae gdkggsadna anvgsgefaa attgeqepvr
      601 egvlrrcgdm tllgsdeply ipitqepapm tedmleqhae alvklgsnpe gamlrarmqs
      661 aclfsdmeaf kaanpgasls dfvrwysprd wvaplvdqat gqvieeghls hrmqlpgntw
      721 qevwetaqpm pahrqkrlfd dtkeaekvly yllslhprei vkllmpilih calahiidha
      781 hegprslttf leqtvtkvas qlrgkepkyk ellsllyeae vavarctslr kkfyrenstd
      841 swatspedig phrqpsttss hlsssknqae tdvnkfvldl vdnpevsipg agkgsvgklv
      901 sqlfvdslkl qdsdaaseae edseckfpsp vareyilrtr hsrptpasrp tpqrmfcvma
      961 gndfrlagaf thdtvfy
//
LOCUS       XP_077502333             879 aa            linear   INV 25-NOV-2025
DEFINITION  leucine-rich repeat and fibronectin type-III domain-containing
            protein hattifattener [Amblyomma americanum].
ACCESSION   XP_077502333
VERSION     XP_077502333.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646207.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..879
                     /product="leucine-rich repeat and fibronectin type-III
                     domain-containing protein hattifattener"
                     /calculated_mol_wt=96463
     Region          87..142
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          87..106
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          107..131
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <131..>337
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          132..155
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          156..171
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          179..202
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          203..225
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          226..249
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          250..298
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          299..322
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          323..344
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          348..360
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          358..409
                     /region_name="LRRCT"
                     /note="Leucine rich repeat C-terminal domain; smart00082"
                     /db_xref="CDD:214507"
     CDS             1..879
                     /gene="haf"
                     /coded_by="XM_077646207.1:13..2652"
                     /db_xref="GeneID:144113233"
ORIGIN      
        1 mcplpssqdq rcpgmwllls aalallaasp gvrakcpwsr dlvdlhadci caysstqrls
       61 vqcspvnfar lmnalhasaq nvpidllhin nstveelpdg afakldiqsl hlarcqlrhv
      121 serallglen slaslslpdn rleevpvaal rrlaslrqld lssnairhvp dgafsglpln
      181 tlkladneli iaddafaglq dslknlnlkg tgqervpraa aqltslafld laqnkiaqla
      241 pedlggmhtl talnlernri vkidadafag indtlsslsl lnnllvefps qalatltelr
      301 vldlgfngir nlpddafann pfltllaldg npmatiplep frhlnttlrg isiggpylec
      361 dcrirwiaew iankdlqvts rernpqycgk pdhfkrrtfa kiepseficn vsttttpppp
      421 pttvslttem etepavatst rspptttpat tttttttttt ttttavppve pvitttvpep
      481 tsvhidellv aetttfrepf vsssesaadv ityapeltda papsqrqasd khvrpllssp
      541 rnqdnevpsn kvplvphivn aqrkdsavti ewqtegespl gfqvvyrlfg ekiyrhgpal
      601 rpdqrkytmq nlptneclvv cvvnlqdaqd igiddvlpsq ckelkleryv iahldkiiia
      661 ssaavcgiii laviiflccw rkkqgrsssp ksrlplptst vhptvkptde wetvsmyssr
      721 siprarmyhm dtangsvnqs fvmddnrshi shfshmpngy sskarstadg qshrsysqms
      781 nkyrglgnpe vrksqqslsa lsgthsflda hvgqntrpla ngkkrvlhng rlasassmhs
      841 lneydsdwna rtenwkdnev diyvgqshva pshskyhhr
//
LOCUS       XP_077502334             602 aa            linear   INV 25-NOV-2025
DEFINITION  2',5'-phosphodiesterase 12 [Amblyomma americanum].
ACCESSION   XP_077502334
VERSION     XP_077502334.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646208.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..602
                     /product="2',5'-phosphodiesterase 12"
                     /calculated_mol_wt=68385
     Region          283..601
                     /region_name="EEP"
                     /note="Exonuclease-Endonuclease-Phosphatase (EEP) domain
                     superfamily; cl00490"
                     /db_xref="CDD:469791"
     Site            order(287,337,445,489,491,554,591..592)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197306"
     Site            order(337,591)
                     /site_type="other"
                     /note="putative metal binding site [ion binding]"
                     /db_xref="CDD:197306"
     Site            order(445,491,592)
                     /site_type="other"
                     /note="putative phosphate binding site [ion binding]"
                     /db_xref="CDD:197306"
     CDS             1..602
                     /gene="LOC144113234"
                     /coded_by="XM_077646208.1:168..1976"
                     /db_xref="GeneID:144113234"
ORIGIN      
        1 mifvrflssk lphfswqcki rmssaavaae kyayvshnse rkllrlsfky asrdvkerqy
       61 tlnrslteel rsvlnriain ianveegkkk rkrkkngcsd edatksesqe elpvsleyng
      121 vpvsgdvans dawkegsilq igehrysiav napavksvrl pkyimagfpv harielehcd
      181 lhdcemtwfm tikrgqtfpn hevykannml fvdthqrssf lvpetadvgr yvllrvipcv
      241 gsrkgmpvev vststvevgp gfcpfearqy fttfftlpgr frcisynlla dvyadtsysr
      301 svlfpycasy aldlcyrkql ltkeilgykg dliclqevdr kvfredlepi lgangflgry
      361 tekcspmaeg vacffrsskf relevyssvl atalveeqal sdittsisqn qslrdrilnl
      421 ptafqvllle plerpkrlll vanthlyyhp dsdhirllqa yscirlvewl raeyaerygv
      481 epavvfagdf nscpafgvyq lmtcgrvsqh crdwysneee avvgleavqh iplasacgtp
      541 aytnytkgfq gcldyifydy mqlvresvvp tplhqqvtqe eglpsvhfps dhvaqvatlr
      601 wl
//
LOCUS       XP_077502335             256 aa            linear   INV 25-NOV-2025
DEFINITION  pre-miRNA 5'-monophosphate methyltransferase isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502335
VERSION     XP_077502335.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646209.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..256
                     /product="pre-miRNA 5'-monophosphate methyltransferase
                     isoform X1"
                     /calculated_mol_wt=28798
     Region          <54..182
                     /region_name="UbiG"
                     /note="2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1,
                     4-benzoquinol methylase [Coenzyme transport and
                     metabolism]; COG2227"
                     /db_xref="CDD:441829"
     Region          146..252
                     /region_name="Bin3"
                     /note="Bicoid-interacting protein 3 (Bin3); pfam06859"
                     /db_xref="CDD:462022"
     CDS             1..256
                     /gene="LOC144113235"
                     /coded_by="XM_077646209.1:20..790"
                     /db_xref="GeneID:144113235"
ORIGIN      
        1 maaptpergg sgsrakygnf nnyyqfnkpe erlkmlpkdl vaycnlsdqs qvvaldigcn
       61 sgvkssahai lcknveltth lyrnlassgm tdvkmlaidi dssliecakk ncsypeaiey
      121 mclditsnas vesltaylek lgrdafdvtf cfsvtmwihl nhgdnglkqf letvsknthf
      181 llveaqlwkc yrsasrrmrr snetefqnle alsmnvnved nihdflqgrc glqvvecfgq
      241 tqwgrkvtly krkeav
//
LOCUS       XP_077502336             243 aa            linear   INV 25-NOV-2025
DEFINITION  pre-miRNA 5'-monophosphate methyltransferase isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502336
VERSION     XP_077502336.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646210.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..243
                     /product="pre-miRNA 5'-monophosphate methyltransferase
                     isoform X2"
                     /calculated_mol_wt=27446
     Region          52..>176
                     /region_name="Methyltransf_31"
                     /note="Methyltransferase domain; pfam13847"
                     /db_xref="CDD:463998"
     Region          133..239
                     /region_name="Bin3"
                     /note="Bicoid-interacting protein 3 (Bin3); pfam06859"
                     /db_xref="CDD:462022"
     CDS             1..243
                     /gene="LOC144113235"
                     /coded_by="XM_077646210.1:20..751"
                     /db_xref="GeneID:144113235"
ORIGIN      
        1 maaptpergg sgsrakygnf nnyyqfnkpe erlkmlpkdl vaycnlsdqs qvvaldigcn
       61 sgeltthlyr nlassgmtdv kmlaididss liecakkncs ypeaieymcl ditsnasves
      121 ltayleklgr dafdvtfcfs vtmwihlnhg dnglkqflet vsknthfllv eaqlwkcyrs
      181 asrrmrrsne tefqnleals mnvnvednih dflqgrcglq vvecfgqtqw grkvtlykrk
      241 eav
//
LOCUS       XP_077502337             240 aa            linear   INV 25-NOV-2025
DEFINITION  cilia- and flagella-associated protein 298-B [Amblyomma
            americanum].
ACCESSION   XP_077502337
VERSION     XP_077502337.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646211.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..240
                     /product="cilia- and flagella-associated protein 298-B"
                     /calculated_mol_wt=26762
     Region          148..>207
                     /region_name="CFAP298"
                     /note="Cilia- and flagella-associated protein 298;
                     pfam11069"
                     /db_xref="CDD:463214"
     CDS             1..240
                     /gene="LOC144113236"
                     /coded_by="XM_077646211.1:320..1042"
                     /db_xref="GeneID:144113236"
ORIGIN      
        1 mvklkvqcgn avqflvetsl sttldaliqd vtdiysdvlv irlaaanies farrlekqpn
       61 apkvtastla epsvilakvt aealarvsnt qlesgktvtk sltentkdml kttlstldlp
      121 kamegernvl lvnmdkmdve nslsgddakl wwagkelcrg nelrkyfgsn ekttvtvals
      181 sqppersksv aeaqfnefll srmkrqkdle elepdeetqm hdryslrkyv hgledvkwkp
//
LOCUS       XP_077502338             100 aa            linear   INV 25-NOV-2025
DEFINITION  translocase of inner membrane 10 [Amblyomma americanum].
ACCESSION   XP_077502338
VERSION     XP_077502338.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646212.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..100
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..100
                     /product="translocase of inner membrane 10"
                     /calculated_mol_wt=11313
     Region          13..75
                     /region_name="zf-Tim10_DDP"
                     /note="Tim10/DDP family zinc finger; pfam02953"
                     /db_xref="CDD:460764"
     CDS             1..100
                     /gene="Tim10"
                     /coded_by="XM_077646212.1:334..636"
                     /db_xref="GeneID:144113237"
ORIGIN      
        1 maanmqldpt klqlvadlei emmsdmynrm taacqkkcip tkyreadlsk gesvcldrcv
       61 akyldiheri gkklttlsmq deelmkkmqe qqstaqahak
//
LOCUS       XP_077502339             418 aa            linear   INV 25-NOV-2025
DEFINITION  actin-related protein 3 [Amblyomma americanum].
ACCESSION   XP_077502339
VERSION     XP_077502339.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646213.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..418
                     /product="actin-related protein 3"
                     /calculated_mol_wt=47044
     Region          7..410
                     /region_name="ASKHA_NBD_Arp3-like"
                     /note="nucleotide-binding domain (NBD) of actin-related
                     protein3 (Arp3) and similar proteins; cd10221"
                     /db_xref="CDD:466822"
     CDS             1..418
                     /gene="Arp3"
                     /coded_by="XM_077646213.1:101..1357"
                     /db_xref="GeneID:144113238"
ORIGIN      
        1 magrlpaivi dngtgytklg fagnkepqfi ipsaiaires akigdqatrr lakgiddldf
       61 figdealdaa gyavkypirh givedwdlme rfweqsifky lraepedhyf lltepplntp
      121 enreytaeim fesfnvpgly iavqavlala aswtsrqmgd rtltglvids gdgvthcipv
      181 aegyvigsci khipiagrnv tyfiqnllre revgippeqs letakaikek lcyicpdiak
      241 efsrydqeps kwikkyegin aitkqkfsvd vgyerflgpe iffhpefsnp dfttpiseiv
      301 dtcvqscpid vrrplykniv lsggstmfkd fgrrlqrdlk rvvdarlkfs eklsggritp
      361 kpmevqvish hmqryavwfg gsmlastpef yqvchtkkay eecgpsicrh npvfgamt
//
LOCUS       XP_077502340            1029 aa            linear   INV 25-NOV-2025
DEFINITION  pre-mRNA-splicing factor CWC22 homolog isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502340
VERSION     XP_077502340.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646214.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1029
                     /product="pre-mRNA-splicing factor CWC22 homolog isoform
                     X1"
                     /calculated_mol_wt=117666
     Region          501..682
                     /region_name="MIF4G"
                     /note="Middle domain of eukaryotic initiation factor 4G
                     (eIF4G); smart00543"
                     /db_xref="CDD:214713"
     Region          786..892
                     /region_name="MA3"
                     /note="MA3 domain; pfam02847"
                     /db_xref="CDD:397128"
     CDS             1..1029
                     /gene="LOC144113239"
                     /coded_by="XM_077646214.1:148..3237"
                     /db_xref="GeneID:144113239"
ORIGIN      
        1 mfhsvlqvrn nplacvcrma dkgdhvlkst vpkdpdclvl gnespvqddv tgektseaqd
       61 vpnrfqqwdc ppwdrwsrer stdsrersvd srdsatrerd fryaeerkrd rergrreevi
      121 lyrhrdsesa nsdsteeeev qcanespvka atssvakdna ksrktlsqdv lpasrtsven
      181 mehssapald thlktaktfk nspvgkeatr gtelakeaiq gtcmagkknr savhqrlddt
      241 hsktsrnsgk cppmkqdhqm ghasdhssds dndhvrhtgh drkkerrret dpdrkrdadh
      301 errrndgghd rerdsdyhrr ekrhhdhrrd hdrdrdrred wdedrkqdrh rrddhkrirr
      361 derderhreg hgkrddhksd hdrsrkgdkr ddeegsgawr mdhkndrdrs rrgdrkdede
      421 wsgasrddhs krggrdeveg lkrarksspd aeiepprkkd vitgrtggay ipparlrmmq
      481 eqitdkssve yqriawealk ksinglvnka nvgnmdviik elmrenlvrg rgllcrtimt
      541 aqaasptfth vyaamvavin trfpqtgeli lkrlviqfrr sfrrndkltc masvrfvahl
      601 lnqqvahevl aleiltlllg nptddsveva vaflkecgmk lteltpkgvh aiferlrsil
      661 heasldkrvq ymievmfair kdgfsahpsv vkelelveee sqythllald dtldgedmln
      721 vfkfdpeyie neekyaeiak dilgsdeegd esgdesddee eeeeeeaqgg engeiidnte
      781 tnlvalrrti yltiqsslnf eecahkllkl elkpgqvsem chmildccaq qrtyekffgl
      841 laqrfcllkk eyaepfeqif lssydtihrf etnklrnvak ffahllftda ipwtvlshvk
      901 lteddttsss rifikilfqe lceymglpkl nerikdptlq dafeglfprn lpkntrfain
      961 fftsiglggl tadlrehlks apktapvpav iasappaskn ktsssssdss sdssssdsss
     1021 dsssdssss
//
LOCUS       XP_077502341            1011 aa            linear   INV 25-NOV-2025
DEFINITION  pre-mRNA-splicing factor CWC22 homolog isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502341
VERSION     XP_077502341.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646215.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1011
                     /product="pre-mRNA-splicing factor CWC22 homolog isoform
                     X2"
                     /calculated_mol_wt=115597
     Region          483..664
                     /region_name="MIF4G"
                     /note="Middle domain of eukaryotic initiation factor 4G
                     (eIF4G); smart00543"
                     /db_xref="CDD:214713"
     Region          768..874
                     /region_name="MA3"
                     /note="MA3 domain; pfam02847"
                     /db_xref="CDD:397128"
     CDS             1..1011
                     /gene="LOC144113239"
                     /coded_by="XM_077646215.1:138..3173"
                     /db_xref="GeneID:144113239"
ORIGIN      
        1 madkgdhvlk stvpkdpdcl vlgnespvqd dvtgektsea qdvpnrfqqw dcppwdrwsr
       61 erstdsrers vdsrdsatre rdfryaeerk rdrergrree vilyrhrdse sansdsteee
      121 evqcanespv kaatssvakd naksrktlsq dvlpasrtsv enmehssapa ldthlktakt
      181 fknspvgkea trgtelakea iqgtcmagkk nrsavhqrld dthsktsrns gkcppmkqdh
      241 qmghasdhss dsdndhvrht ghdrkkerrr etdpdrkrda dherrrndgg hdrerdsdyh
      301 rrekrhhdhr rdhdrdrdrr edwdedrkqd rhrrddhkri rrderderhr eghgkrddhk
      361 sdhdrsrkgd krddeegsga wrmdhkndrd rsrrgdrkde dewsgasrdd hskrggrdev
      421 eglkrarkss pdaeiepprk kdvitgrtgg ayipparlrm mqeqitdkss veyqriawea
      481 lkksinglvn kanvgnmdvi ikelmrenlv rgrgllcrti mtaqaasptf thvyaamvav
      541 intrfpqtge lilkrlviqf rrsfrrndkl tcmasvrfva hllnqqvahe vlaleiltll
      601 lgnptddsve vavaflkecg mklteltpkg vhaiferlrs ilheasldkr vqymievmfa
      661 irkdgfsahp svvkelelve eesqythlla lddtldgedm lnvfkfdpey ieneekyaei
      721 akdilgsdee gdesgdesdd eeeeeeeeaq ggengeiidn tetnlvalrr tiyltiqssl
      781 nfeecahkll klelkpgqvs emchmildcc aqqrtyekff gllaqrfcll kkeyaepfeq
      841 iflssydtih rfetnklrnv akffahllft daipwtvlsh vklteddtts ssrifikilf
      901 qelceymglp klnerikdpt lqdafeglfp rnlpkntrfa infftsiglg gltadlrehl
      961 ksapktapvp aviasappas knktsssssd sssdssssds ssdsssdsss s
//
LOCUS       XP_077502342             636 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113243 [Amblyomma americanum].
ACCESSION   XP_077502342
VERSION     XP_077502342.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646216.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..636
                     /product="uncharacterized protein LOC144113243"
                     /calculated_mol_wt=69881
     Region          <105..>459
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          <430..542
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..636
                     /gene="LOC144113243"
                     /coded_by="XM_077646216.1:87..1997"
                     /db_xref="GeneID:144113243"
ORIGIN      
        1 mqrlveehsn vfadyeslvl qhrtekeekd qliaqlrtla sklcdpsdld dgsalklfea
       61 lssavqqlsd ersslndrer aakttiealm gerdalmaqn rvlveklkdy nelqqakesl
      121 ehhltihkdf aknitrevhq ltsaidalkq vvqesaiarg pfeatvqtia reavklhaqi
      181 fydhwsrsqg vtsdmsdela rkkeevkkls aecarmdsvi reqmaeiegl csgitnfeql
      241 aadsaddlar vqaeaeslke slaaaqmeae ereeaallll ekdtqiaqle gelqekreel
      301 aslvqekaav deserlrecn qkwkeivssi skkleqeknw resmeiqflk tskgeialkp
      361 sepsrqalak letenkhlka evsklqekvc sltneiaetr nsaalkleri erlyreakee
      421 nqfmkravia asagvtpgrq astavqrgsr papaapqdvp kcmptqecpf tssflhtpyk
      481 pstsvaqaps liapafaeap asstfrgrvv vhepalrqeg pvvtpesspc tspglevaem
      541 padssqesss sevslselas iceaipvkpk pptgnaggks cstkshavaa tmaskhlqea
      601 ktpvkasprk rkrpctasav passkkskle kkedss
//
LOCUS       XP_077502343             560 aa            linear   INV 25-NOV-2025
DEFINITION  ASCIZ zinc finger protein isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502343
VERSION     XP_077502343.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646217.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..560
                     /product="ASCIZ zinc finger protein isoform X1"
                     /calculated_mol_wt=61732
     Site            order(31,33,35,37..38,41..42,46,59,61,64..65,68..69,84,86,
                     88,90..91,94..95,100)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          54..70
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          80..101
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..560
                     /gene="Asciz"
                     /coded_by="XM_077646217.1:86..1768"
                     /db_xref="GeneID:144113246"
ORIGIN      
        1 mkakaepqlp narksfcctv ssccsrdgqp klftsqkllt qhfikvhaek kyscskcgkg
       61 fgaewlskhh eatcgtswrc scgasyqnre alltharrrs htlpfelkrg dackrrqpsr
      121 qslqpiivpv atqviiviqs prerssdskd nlaqsqtkvp sqwrsiipka spvgdlsshg
      181 ldtpssslai ekvhqssqtn vsckcrhtkd sciqacsmeg stkdaselvn lplkaskrsr
      241 amktavhtqt yesgvhhtgi qpllskgvsk karhrcsiqt qtiesavdra qkarakrnia
      301 rpeprkldke iatnelwsdl dqilfngcat pykewmdifs rsssatqtld sdallkdlcn
      361 pypsscslfl dgprgsfsqi glpavpleqg ldvispkpgm lpilapicep gvagsledsl
      421 hdvghilsse tqtdylfdsl lienmqqtaa qtdfpscshn etqtnedvsf egtdvlhmet
      481 qtsgflfcdf esvdietqtp wdhldfsldd lvvepdftmk kdaesqigdy qlvcpwhclr
      541 pgvenskgcn nmqtqtsgsi
//
LOCUS       XP_077502344             123 aa            linear   INV 25-NOV-2025
DEFINITION  ASCIZ zinc finger protein isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502344
VERSION     XP_077502344.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646218.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..123
                     /product="ASCIZ zinc finger protein isoform X2"
                     /calculated_mol_wt=13760
     CDS             1..123
                     /gene="Asciz"
                     /coded_by="XM_077646218.1:86..457"
                     /db_xref="GeneID:144113246"
ORIGIN      
        1 mkakaepqlp narksfcctv ssccsrdgqp klftsqkllt qhfikvhaek kyscskcgkg
       61 fgaewlskhh eatcgtswrc scgasyqnre alltharrrs htlpfelkrg dacnslkssf
      121 wfi
//
LOCUS       XP_077502345             794 aa            linear   INV 25-NOV-2025
DEFINITION  spire type actin nucleation factor isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502345
VERSION     XP_077502345.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646219.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..794
                     /product="spire type actin nucleation factor isoform X1"
                     /calculated_mol_wt=88770
     Region          19..229
                     /region_name="KIND"
                     /note="Kinase non-catalytic C-lobe domain; pfam16474"
                     /db_xref="CDD:465129"
     Region          251..281
                     /region_name="WH2_Spire_1-2_r1"
                     /note="first tandem Wiskott-Aldrich Syndrome Homology
                     (WASP) region 2 (WH2 motif) repeat of protein Spire
                     homologs 1 and 2; cd22065"
                     /db_xref="CDD:409208"
     Site            order(264..265,267..269,271..273,277..280)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409208"
     Region          277..280
                     /region_name="actin-binding sequence"
                     /note="actin-binding sequence [structural motif]"
                     /db_xref="CDD:409208"
     Region          290..321
                     /region_name="WH2_Spire1_r2-like"
                     /note="second tandem Wiskott-Aldrich Syndrome Homology
                     (WASP) region 2 (WH2 motif) repeat of protein Spire
                     homolog 1 (Spir1), and related proteins; cd22078"
                     /db_xref="CDD:409221"
     Site            order(295..296,298..300,302..304,310..313)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409221"
     Region          310..313
                     /region_name="actin-binding sequence"
                     /note="actin-binding sequence [structural motif]"
                     /db_xref="CDD:409221"
     Region          323..348
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(327..328,330..332,334..336,341..344)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     Region          567..602
                     /region_name="FYVE_SPIR"
                     /note="FYVE-related domain found in Spir proteins, Spire1
                     and Spire2; cd15748"
                     /db_xref="CDD:277287"
     CDS             1..794
                     /gene="spir"
                     /coded_by="XM_077646219.1:71..2455"
                     /db_xref="GeneID:144113247"
ORIGIN      
        1 masedanapk cdlegdgcvr lssileafaa pireeqawam cyqtskcmck ewmadnrgcy
       61 cltdtsqlri hkdgsvhstt vrdlgpastr piatseyklv cslglvlfha leyglrsdee
      121 hvlsrpfeml ldrmtsadpe lssdaelssd ddsgeedchd egiekdsged dpgggdsgag
      181 ksphragltl akvlemcash lqnpsqadmh ykavcralva ealelstflq kissgtkefi
      241 kchrsdpdts dldglqfhdw amlwmqvire lrqgvklkkv dvethlhpve yemtpyemll
      301 ddirsqrykl nkvmvngdlp prvkkdahal ilefirsrpp lfpvsrrklk paprretsah
      361 drlmadirqp qvlrpvrkra tasscvetsr trlddsdstp qprrrlimad islhlsssfd
      421 eddlddspts pvaeqpqslp vqlrrtpnrp tklqwqqkhs sgdgadvttt avlkqklerr
      481 hsitvcesps rvsntsitqd garrpdklsl strhesgken wttaqweksm eclsltleev
      541 vhirnvltka elesllankp lyedvekrrv cftckktkfs ffrpwgvkck lcerticdkc
      601 stkmhiptdr fdripvymlc ptateeespn hsplpvafqf tsgsapsspi lnrptfntkl
      661 nvpvyappts tprprvaklr haasvcsprt ggsedttsqe tpvrrppllr srtfqhkass
      721 pqeeqlrgpl mtvcrdckgm irhiilasrt dmaksqrkac kpplclspvl pspagtpdap
      781 masagkrplf vrcq
//
LOCUS       XP_077502346             793 aa            linear   INV 25-NOV-2025
DEFINITION  spire type actin nucleation factor isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502346
VERSION     XP_077502346.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646220.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..793
                     /product="spire type actin nucleation factor isoform X2"
                     /calculated_mol_wt=88603
     Region          19..229
                     /region_name="KIND"
                     /note="Kinase non-catalytic C-lobe domain; pfam16474"
                     /db_xref="CDD:465129"
     Region          251..281
                     /region_name="WH2_Spire_1-2_r1"
                     /note="first tandem Wiskott-Aldrich Syndrome Homology
                     (WASP) region 2 (WH2 motif) repeat of protein Spire
                     homologs 1 and 2; cd22065"
                     /db_xref="CDD:409208"
     Site            order(264..265,267..269,271..273,277..280)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409208"
     Region          277..280
                     /region_name="actin-binding sequence"
                     /note="actin-binding sequence [structural motif]"
                     /db_xref="CDD:409208"
     Region          290..321
                     /region_name="WH2_Spire1_r2-like"
                     /note="second tandem Wiskott-Aldrich Syndrome Homology
                     (WASP) region 2 (WH2 motif) repeat of protein Spire
                     homolog 1 (Spir1), and related proteins; cd22078"
                     /db_xref="CDD:409221"
     Site            order(295..296,298..300,302..304,310..313)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409221"
     Region          310..313
                     /region_name="actin-binding sequence"
                     /note="actin-binding sequence [structural motif]"
                     /db_xref="CDD:409221"
     Region          323..348
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(327..328,330..332,334..336,341..344)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     Region          567..602
                     /region_name="FYVE_SPIR"
                     /note="FYVE-related domain found in Spir proteins, Spire1
                     and Spire2; cd15748"
                     /db_xref="CDD:277287"
     CDS             1..793
                     /gene="spir"
                     /coded_by="XM_077646220.1:71..2452"
                     /db_xref="GeneID:144113247"
ORIGIN      
        1 masedanapk cdlegdgcvr lssileafaa pireeqawam cyqtskcmck ewmadnrgcy
       61 cltdtsqlri hkdgsvhstt vrdlgpastr piatseyklv cslglvlfha leyglrsdee
      121 hvlsrpfeml ldrmtsadpe lssdaelssd ddsgeedchd egiekdsged dpgggdsgag
      181 ksphragltl akvlemcash lqnpsqadmh ykavcralva ealelstflq kissgtkefi
      241 kchrsdpdts dldglqfhdw amlwmqvire lrqgvklkkv dvethlhpve yemtpyemll
      301 ddirsqrykl nkvmvngdlp prvkkdahal ilefirsrpp lfpvsrrklk paprretsah
      361 drlmadirqp qvlrpvrkra tasscvetsr trlddsdstp qprrrlimad islhlsssfd
      421 eddlddspts pvaeqpqslp vqlrrtpnrp tklqwqqkhs sgdgadvttt avlkqklerr
      481 hsitvcesps rvsntsitqd garrpdklsl strhesgken wttaqweksm eclsltleev
      541 vhirnvltka elesllankp lyedvekrrv cftckktkfs ffrpwgvkck lcerticdkc
      601 stkmhiptdr fdripvymlc ptateeespn hsplpvafqf tsgsapsspi lnrptfntkl
      661 nvpvyappts tprprvaklr haasvcsprt ggsedttsqe tpvrrppllr srtfqhkass
      721 pqeeqlrgpl mtvcrdckgm irhiilasrt dmaksqrkac kpplclspvl pspagtpdap
      781 masagrrsdp rgh
//
LOCUS       XP_077502347             785 aa            linear   INV 25-NOV-2025
DEFINITION  spire type actin nucleation factor isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502347
VERSION     XP_077502347.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646221.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..785
                     /product="spire type actin nucleation factor isoform X3"
                     /calculated_mol_wt=87810
     Region          19..229
                     /region_name="KIND"
                     /note="Kinase non-catalytic C-lobe domain; pfam16474"
                     /db_xref="CDD:465129"
     Region          251..281
                     /region_name="WH2_Spire_1-2_r1"
                     /note="first tandem Wiskott-Aldrich Syndrome Homology
                     (WASP) region 2 (WH2 motif) repeat of protein Spire
                     homologs 1 and 2; cd22065"
                     /db_xref="CDD:409208"
     Site            order(264..265,267..269,271..273,277..280)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409208"
     Region          277..280
                     /region_name="actin-binding sequence"
                     /note="actin-binding sequence [structural motif]"
                     /db_xref="CDD:409208"
     Region          290..>314
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(295..296,298..300,302..304,310..313)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     Region          558..593
                     /region_name="FYVE_SPIR"
                     /note="FYVE-related domain found in Spir proteins, Spire1
                     and Spire2; cd15748"
                     /db_xref="CDD:277287"
     CDS             1..785
                     /gene="spir"
                     /coded_by="XM_077646221.1:71..2428"
                     /db_xref="GeneID:144113247"
ORIGIN      
        1 masedanapk cdlegdgcvr lssileafaa pireeqawam cyqtskcmck ewmadnrgcy
       61 cltdtsqlri hkdgsvhstt vrdlgpastr piatseyklv cslglvlfha leyglrsdee
      121 hvlsrpfeml ldrmtsadpe lssdaelssd ddsgeedchd egiekdsged dpgggdsgag
      181 ksphragltl akvlemcash lqnpsqadmh ykavcralva ealelstflq kissgtkefi
      241 kchrsdpdts dldglqfhdw amlwmqvire lrqgvklkkv dvethlhpve yemtpyemll
      301 ddirsqrykl nkvmqrtpev aiqekvfklp plfpvsrrkl kpaprretsa hdrlmadirq
      361 pqvlrpvrkr atasscvets rtrlddsdst pqprrrlima dislhlsssf deddlddspt
      421 spvaeqpqsl pvqlrrtpnr ptklqwqqkh ssgdgadvtt tavlkqkler rhsitvcesp
      481 srvsntsitq dgarrpdkls lstrhesgke nwttaqweks meclsltlee vvhirnvltk
      541 aelesllank plyedvekrr vcftckktkf sffrpwgvkc klcerticdk cstkmhiptd
      601 rfdripvyml cptateeesp nhsplpvafq ftsgsapssp ilnrptfntk lnvpvyappt
      661 stprprvakl rhaasvcspr tggsedttsq etpvrrppll rsrtfqhkas spqeeqlrgp
      721 lmtvcrdckg mirhiilasr tdmaksqrka ckpplclspv lpspagtpda pmasagkrpl
      781 fvrcq
//
LOCUS       XP_077502348             128 aa            linear   INV 25-NOV-2025
DEFINITION  programmed cell death 5 [Amblyomma americanum].
ACCESSION   XP_077502348
VERSION     XP_077502348.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646222.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..128
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..128
                     /product="programmed cell death 5"
                     /calculated_mol_wt=14150
     Region          9..115
                     /region_name="dsDNA_bind"
                     /note="Double-stranded DNA-binding domain; pfam01984"
                     /db_xref="CDD:460404"
     CDS             1..128
                     /gene="PDCD-5"
                     /coded_by="XM_077646222.1:107..493"
                     /db_xref="GeneID:144113248"
ORIGIN      
        1 mddeelaalr arrlgdlqkg lggngpsnad reaerkakae dfknqvlnqa ltqeararln
       61 tialakpeka alvenmlvrm vqmgqiqskl pesglislle kvseqtqkkt tvkfdrrrag
      121 ldsdeeey
//
LOCUS       XP_077502349             724 aa            linear   INV 25-NOV-2025
DEFINITION  C-Maf-inducing protein-like [Amblyomma americanum].
ACCESSION   XP_077502349
VERSION     XP_077502349.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646223.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..724
                     /product="C-Maf-inducing protein-like"
                     /calculated_mol_wt=79914
     Region          <154..201
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          <601..722
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     Region          615..638
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          639..663
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          664..687
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          688..712
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..724
                     /gene="LOC144113249"
                     /coded_by="XM_077646223.1:402..2576"
                     /db_xref="GeneID:144113249"
ORIGIN      
        1 meeqpnnsrs vqepgsrrll ppdeqssgsv agssaqtnfv rnvfstggaq tsvdsmssds
       61 sdcsalagts aerpgspqgp asappaaacc aqgpkfklth egdvqlcrln hsrtviskil
      121 sskflrrwet hkvllgtsem sskmpsgfme qpvsysvmed vyslcrwdps hkfclrvvip
      181 egslllqaan gylrdqwlhs ilwkksllkh ekilmtsrri evvtkelknl velalttplq
      241 dkniyqrple iiskflvlsq ewlygkavet vikailplle rttptpeice yfckhcrecp
      301 rsklvfemyt pvvqrilkrn mdfgkypmtr llvqeyiqsl ncqnsgleav eefvrsvhge
      361 gstcphprvl pnlvatvlag vfalfearrn peehndashs tsdweakhdc fvkvldvivn
      421 yedwrlplgq llqplpfpdd algcqrftlg lepiveclgt dprcevhsvv lgaregkdgw
      481 lqlyspgsga cqdggalwsq llgtlvgcgc grrraflgpl akwlealcer avagdtacqm
      541 tlcamlelga lesqearlqa saalqssphy galclrqgqa rqlqqkggpt kltlparstd
      601 adlekllscg sfgnleclsl aftqvtsaca ehliklpslr ylnlwstqfg dsglkliseh
      661 lpklqvlnlc etpvtdkgls slesmkslrk lnlnstnlsa qtfeclkekl palqecdvry
      721 tdaw
//
LOCUS       XP_077502350             452 aa            linear   INV 25-NOV-2025
DEFINITION  coiled-coil domain-containing protein 102A isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502350
VERSION     XP_077502350.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646224.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..452
                     /product="coiled-coil domain-containing protein 102A
                     isoform X1"
                     /calculated_mol_wt=51966
     Region          <67..>265
                     /region_name="COG4913"
                     /note="Uncharacterized conserved protein, contains a
                     C-terminal ATPase domain [Function unknown]"
                     /db_xref="CDD:443941"
     Region          <168..>409
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..452
                     /gene="LOC144113250"
                     /coded_by="XM_077646224.1:373..1731"
                     /db_xref="GeneID:144113250"
ORIGIN      
        1 msshvkrdts yrsydsewea keelrlrele eararaaqme ktmrwwsdct anwrekwskv
       61 rternkaree arllrarvda atkeaaalkr ekheleaevd qlrrefdylr taesgttsrv
      121 sellatssaa sshseqehpd qetssglppq lesdpadqqa slmqlrldet akllfaerqe
      181 knqvakqlek qtgelgqlra kydelrksrq dtlkelsqir aehqdeleci lidledeasg
      241 rtcldkrmte lraqlvqlqg enaaewgrre rleteklale rdnknlrasl qemqeclqrk
      301 aqssstspdq kalqaelqhr tkelldlkha hsklkkvlqe kstelshalr rseqyeaevk
      361 klrgridelk kelataedea dsatnsirkl qrcndelqeq ietlqvqleh lqtsqeeclv
      421 asvhldtvka eeqcaaqasq pappeqprra gs
//
LOCUS       XP_077502351             448 aa            linear   INV 25-NOV-2025
DEFINITION  coiled-coil domain-containing protein 102A isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502351
VERSION     XP_077502351.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646225.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..448
                     /product="coiled-coil domain-containing protein 102A
                     isoform X2"
                     /calculated_mol_wt=51680
     Region          <67..>265
                     /region_name="COG4913"
                     /note="Uncharacterized conserved protein, contains a
                     C-terminal ATPase domain [Function unknown]"
                     /db_xref="CDD:443941"
     Region          <168..>416
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..448
                     /gene="LOC144113250"
                     /coded_by="XM_077646225.1:378..1724"
                     /db_xref="GeneID:144113250"
ORIGIN      
        1 msshvkrdts yrsydsewea keelrlrele eararaaqme ktmrwwsdct anwrekwskv
       61 rternkaree arllrarvda atkeaaalkr ekheleaevd qlrrefdylr taesgttsrv
      121 sellatssaa sshseqehpd qetssglppq lesdpadqqa slmqlrldet akllfaerqe
      181 knqvakqlek qtgelgqlra kydelrksrq dtlkelsqir aehqdeleci lidledeasg
      241 rtcldkrmte lraqlvqlqg enaaewgrre rleteklale rdnknlrasl qemqeclqrk
      301 aqssstspdq kalqaelqhr tkelldlkha hsklkkvlqe kstelshalr rseqyeaevk
      361 klrgridelk kelataedea dsatnsirkl qrcndelqeq ietlqvqleh lqtrlknsvp
      421 hklhslhhls srggqaadll sedevged
//
LOCUS       XP_077502352             213 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113251 [Amblyomma americanum].
ACCESSION   XP_077502352
VERSION     XP_077502352.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646226.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..213
                     /product="uncharacterized protein LOC144113251"
                     /calculated_mol_wt=23699
     Region          67..88
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(67,70,83,88)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          95..115
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(95,98,111,115)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(100,102,104,106..107,110..111,114,128,130,134..135,
                     138..139,142,156,158,160,162..163,166..167,170)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          123..143
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          147..>209
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          151..171
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          179..198
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..213
                     /gene="LOC144113251"
                     /coded_by="XM_077646226.1:31..672"
                     /db_xref="GeneID:144113251"
ORIGIN      
        1 mwlrcrwvsd prayvppcpp yhqphhqfma ppvdpcfmgt pqpvpqavpa aaaagppgld
       61 agtgfwcdlc gkvfdrrqal lshgcrshgi crfvcthcga afrqssqltk hnrthtgerp
      121 fpcqvcqrsf akksslvehv rthtgerpyr chicqatfsr kahlvghtrt htgerpfqcp
      181 acpkafatss alnqhrrvth rspendnspr pll
//
LOCUS       XP_077502353             569 aa            linear   INV 25-NOV-2025
DEFINITION  aarF domain containing kinase 5 [Amblyomma americanum].
ACCESSION   XP_077502353
VERSION     XP_077502353.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646227.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..569
                     /product="aarF domain containing kinase 5"
                     /calculated_mol_wt=65409
     Region          159..410
                     /region_name="ADCK1-like"
                     /note="aarF domain containing kinase 1 and similar
                     proteins; cd13969"
                     /db_xref="CDD:270871"
     Site            order(209..213,215,225,227,286,305..308,350,354..355,357,
                     371..372)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:270871"
     CDS             1..569
                     /gene="Adck5"
                     /coded_by="XM_077646227.1:110..1819"
                     /db_xref="GeneID:144113252"
ORIGIN      
        1 mvysvlkmaq qcvrcqalrf ysnapagssk vtavpsarnl rnryvyalvg vslsapfiwy
       61 qtlsppdkrf vrtslggllr faktlriglt iscdytysaw gytegtqeyk dmmkrchqrg
      121 aerilkgcle ngglyiklgq slvalnhllp reyldtlevl hdhalvrskd eiselfqedf
      181 gclpeemfke fnrtpiaaas laqvfkaktl egrdvavkvq yidlqqrfng dlngigilih
      241 ivswmhpdfn fawvldylrs slvkeldfvl eagnmercar dlahlpyvsv pkvlwnktsk
      301 rvltmdfvdg vkisnvegik klgldladvd qkmvsafaeq lfhtgfvhad phpgnvfvkk
      361 gddgkakial ldhglyeyis kenrlslcql wksiimndhs gmkthslelg vanypvfcei
      421 lmqrplqrqt frlrnklsse dvaymrnmvq thfdevmeci rslprpmllv frnintvrsi
      481 tknhghpvdr ftlmariatr rltlnlehvg lmmmlrhwws rtmfelrlrl eywqlrlsll
      541 ylhfllwvrg ptkdieylme lasgdvspm
//
LOCUS       XP_077502354             344 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113254 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502354
VERSION     XP_077502354.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646228.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..344
                     /product="uncharacterized protein LOC144113254 isoform X1"
                     /calculated_mol_wt=37720
     CDS             1..344
                     /gene="LOC144113254"
                     /coded_by="XM_077646228.1:115..1149"
                     /db_xref="GeneID:144113254"
ORIGIN      
        1 msfptveqya stcfcwplqt rclddedend ghalfdtkas gdgaegklpa rrvnfalqpg
       61 rherrvgttg avvvevvasv gappssvqir vgpakhgpvp sasrpslaaa assssscsgs
      121 trrdktsarp pakkdnssdd fehagllasn atvdavlael rarhavqaql adclwvpdfd
      181 agrvrvqrpr fwlmntlfre gvsfmcytrp rpprqlpdev qlrtcvaahd mrlvcgftya
      241 cfrggleltf grfhmsvtlv freglpcvrc levqtiedph iielrgvssp frkfvwagvk
      301 kairrkkefl atavseryke vlqtaigkvd mtavfakrml gprs
//
LOCUS       XP_077502355             313 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113254 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502355
VERSION     XP_077502355.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646229.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..313
                     /product="uncharacterized protein LOC144113254 isoform X2"
                     /calculated_mol_wt=34204
     CDS             1..313
                     /gene="LOC144113254"
                     /coded_by="XM_077646229.1:158..1099"
                     /db_xref="GeneID:144113254"
ORIGIN      
        1 mqalafvgpc klaaltmrmk ttdtrcstrr hqamvgttga vvvevvasvg appssvqirv
       61 gpakhgpvps asrpslaaaa ssssscsgst rrdktsarpp akkdnssddf ehagllasna
      121 tvdavlaelr arhavqaqla dclwvpdfda grvrvqrprf wlmntlfreg vsfmcytrpr
      181 pprqlpdevq lrtcvaahdm rlvcgftyac frggleltfg rfhmsvtlvf reglpcvrcl
      241 evqtiedphi ielrgvsspf rkfvwagvkk airrkkefla tavserykev lqtaigkvdm
      301 tavfakrmlg prs
//
LOCUS       XP_077502356            1181 aa            linear   INV 25-NOV-2025
DEFINITION  nardilysin-like [Amblyomma americanum].
ACCESSION   XP_077502356
VERSION     XP_077502356.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646230.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1181
                     /product="nardilysin-like"
                     /calculated_mol_wt=133982
     Region          175..1045
                     /region_name="Ptr"
                     /note="Secreted/periplasmic Zn-dependent peptidases,
                     insulinase-like [Posttranslational modification, protein
                     turnover, chaperones]; COG1025"
                     /db_xref="CDD:440648"
     CDS             1..1181
                     /gene="LOC144113255"
                     /coded_by="XM_077646230.1:404..3949"
                     /db_xref="GeneID:144113255"
ORIGIN      
        1 mddiikspnd ireyrvitle ngltallvsd hktrphspms csssrhssss sspasskkms
       61 hdcvtvaeel pencfeaaqv aalpkisias sianylnatk hedppqakgt hdqppernae
      121 lcsvsssrpq ssecpfsddt sdesmsdddv scqgseseae sdaqsghhsr adnkqqsnar
      181 kekmaaaalc vgvgsfhepd qlqglahfle hmvfmgsaky prenyfdafl nkhggsdnay
      241 tecertvykm evhqkhlhra ldifanffvs pllrkesmer eleaidnefq lvlpsdtcrl
      301 qqllgsiaye ghpmkkfmwg ntnslknvpe kdgtdvhaal rtffekyyrp sqmtlavqsr
      361 hslddlehmv keifsamptr tpseelisfp isepfpveef tklykvqpvk kvnrvsitwa
      421 lpsllheykt kpleyisyvv ghegvgsila ylrerswalg lvagnegsgf hhnttcslfn
      481 itisiteegl knveevltav fsfvamarqv gpvksifeei qtvadnnfrw ceeespldyv
      541 erlcanmqly ppqhylsget clfeydpvli qkcldelvpc kanimiiscr yqkqgicslk
      601 epyletpyct qevpqewfsa wsnivpdpyf evpqrnkyia sdfslkegne hqsdlpiklh
      661 edqryrlwyk kdtkfnvpka cvyfqlispv myisaenavl mdllcdillq nmsqetnaav
      721 caaldftisv nengliikvl gfneklpllf dvilhhlayf evkqelfdni rkhlhkryyn
      781 lfmkpahlct dtrfsvlqqc hwsniekrmv ikdatvstll cfveqfrkql fleglvhgnl
      841 taseaialae laveklsctp lppcmipdsr vmqiplgnyy crvasfnied hnsmivnyyq
      901 lgpgsvkqht laelmidfme epcfdilrtk sqlgydvncs nrntngivgf tvgvccsaek
      961 facsyvdeqi elflsmfakk iseltqeefa tqvsslikqk scsdlylqee adrhwgeiva
     1021 fdylfdrlqr eidflktltl eefkdwcklf lplqrpgeph rrklsiqivg ygeaaiaesk
     1081 pfgchlphss ssstgdiqnt psegkalsfq lciehmhnih pwkhpkrphd hnsteetsse
     1141 dltyslqfle svvgkeevsy igdvtvfkqg lavyplcrvk s
//
LOCUS       XP_077502357             320 aa            linear   INV 25-NOV-2025
DEFINITION  sirtuin 4 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502357
VERSION     XP_077502357.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646231.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..320
                     /product="sirtuin 4 isoform X1"
                     /calculated_mol_wt=34621
     Region          70..318
                     /region_name="SIRT5_Af1_CobB"
                     /note="Eukaryotic, archaeal and prokaryotic group (class3)
                     which includes human sirtuin SIRT5, Archaeoglobus fulgidus
                     Sir2-Af1, and E. coli CobB; and are members of the SIR2
                     family of proteins, silent information regulator 2 (Sir2)
                     enzymes which catalyze NAD...; cd01412"
                     /db_xref="CDD:238703"
     Site            order(79,81..82,89..90,137,159..160,162,177,266,271,
                     293..294,309)
                     /site_type="other"
                     /note="NAD+ binding site [chemical binding]"
                     /db_xref="CDD:238703"
     Site            order(161,177,238,240..244,270..272)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:238703"
     Site            order(185,188,226,229)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:238703"
     CDS             1..320
                     /gene="Sirt4"
                     /coded_by="XM_077646231.1:40..1002"
                     /db_xref="GeneID:144113256"
ORIGIN      
        1 meaaveycvy ffhltaynrl csswmaarvv lrralsvtga llsrpstmak arrqpssdma
       61 afrevfksar hvvaltgagi saesgvptfr gagglwrkyn aqdlatptaf lanpslvwef
      121 yhyrrdlvlq kqpnaahkal aeaeshfekd grtfvvitqn idelhraagt knllelhgtl
      181 fktrctkcgh vavnrdnpit palagkgapd peapeaavpv selprctkct gllrphvvwf
      241 geslepdvla kanvelercd lclvigtssv vypaamfape vaargvpvae fnlestpgte
      301 nfgfhfdgpc gttlpkalal
//
LOCUS       XP_077502358             296 aa            linear   INV 25-NOV-2025
DEFINITION  sirtuin 4 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502358
VERSION     XP_077502358.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646232.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..296
                     /product="sirtuin 4 isoform X2"
                     /calculated_mol_wt=31735
     Region          46..294
                     /region_name="SIRT5_Af1_CobB"
                     /note="Eukaryotic, archaeal and prokaryotic group (class3)
                     which includes human sirtuin SIRT5, Archaeoglobus fulgidus
                     Sir2-Af1, and E. coli CobB; and are members of the SIR2
                     family of proteins, silent information regulator 2 (Sir2)
                     enzymes which catalyze NAD...; cd01412"
                     /db_xref="CDD:238703"
     Site            order(55,57..58,65..66,113,135..136,138,153,242,247,
                     269..270,285)
                     /site_type="other"
                     /note="NAD+ binding site [chemical binding]"
                     /db_xref="CDD:238703"
     Site            order(137,153,214,216..220,246..248)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:238703"
     Site            order(161,164,202,205)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:238703"
     CDS             1..296
                     /gene="Sirt4"
                     /coded_by="XM_077646232.1:146..1036"
                     /db_xref="GeneID:144113256"
ORIGIN      
        1 maarvvlrra lsvtgallsr pstmakarrq pssdmaafre vfksarhvva ltgagisaes
       61 gvptfrgagg lwrkynaqdl atptaflanp slvwefyhyr rdlvlqkqpn aahkalaeae
      121 shfekdgrtf vvitqnidel hraagtknll elhgtlfktr ctkcghvavn rdnpitpala
      181 gkgapdpeap eaavpvselp rctkctgllr phvvwfgesl epdvlakanv elercdlclv
      241 igtssvvypa amfapevaar gvpvaefnle stpgtenfgf hfdgpcgttl pkalal
//
LOCUS       XP_077502359             308 aa            linear   INV 25-NOV-2025
DEFINITION  protein IMPACT-B-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502359
VERSION     XP_077502359.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646233.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..308
                     /product="protein IMPACT-B-like isoform X1"
                     /calculated_mol_wt=34939
     Region          18..113
                     /region_name="RWD_IMPACT"
                     /note="RWD domain of protein IMPACT and related proteins;
                     cd23821"
                     /db_xref="CDD:467657"
     Region          171..279
                     /region_name="UPF0029"
                     /note="Uncharacterized protein family UPF0029; pfam01205"
                     /db_xref="CDD:460111"
     CDS             1..308
                     /gene="LOC144113258"
                     /coded_by="XM_077646233.1:209..1135"
                     /db_xref="GeneID:144113258"
ORIGIN      
        1 msqgmdhiav adnvssqade iealsaiyqe dwkvedaakr vykielcsea sgdklsfqvt
       61 lppeypseap pfyeisapwm sqerkaklla dlddhfynhm gesiiydwie iarkavradp
      121 verrsacasd detpevvesf hllamqpdip sppvdfrgsl nvfhgdiled rrsvfqahla
      181 evhtvreveq vldelktnkk iaeathniwa yrilqteprt vlckdseddg etyagnrllh
      241 lldildvknv livvtrwygg ihlgpdrfkh inklardlle qcgyikkeml tpdskskakq
      301 kkpkgkkt
//
LOCUS       XP_077502360             280 aa            linear   INV 25-NOV-2025
DEFINITION  protein IMPACT-B-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502360
VERSION     XP_077502360.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646234.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..280
                     /product="protein IMPACT-B-like isoform X2"
                     /calculated_mol_wt=31479
     Region          18..113
                     /region_name="RWD_IMPACT"
                     /note="RWD domain of protein IMPACT and related proteins;
                     cd23821"
                     /db_xref="CDD:467657"
     Region          171..>237
                     /region_name="UPF0029"
                     /note="Uncharacterized protein family UPF0029; pfam01205"
                     /db_xref="CDD:460111"
     CDS             1..280
                     /gene="LOC144113258"
                     /coded_by="XM_077646234.1:203..1045"
                     /db_xref="GeneID:144113258"
ORIGIN      
        1 msqgmdhiav adnvssqade iealsaiyqe dwkvedaakr vykielcsea sgdklsfqvt
       61 lppeypseap pfyeisapwm sqerkaklla dlddhfynhm gesiiydwie iarkavradp
      121 verrsacasd detpevvesf hllamqpdip sppvdfrgsl nvfhgdiled rrsvfqahla
      181 evhtvreveq vldelktnkk iaeathniwa yrilqteprt vlckdseddg etyagnrllh
      241 lldlslvgme aftwaqiass tstnwpeisl nsvvtskkkc
//
LOCUS       XP_077502361             248 aa            linear   INV 25-NOV-2025
DEFINITION  protein IMPACT-B-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077502361
VERSION     XP_077502361.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646235.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..248
                     /product="protein IMPACT-B-like isoform X3"
                     /calculated_mol_wt=28064
     Region          18..113
                     /region_name="RWD_IMPACT"
                     /note="RWD domain of protein IMPACT and related proteins;
                     cd23821"
                     /db_xref="CDD:467657"
     Region          171..>242
                     /region_name="UPF0029"
                     /note="Uncharacterized protein family UPF0029; pfam01205"
                     /db_xref="CDD:460111"
     CDS             1..248
                     /gene="LOC144113258"
                     /coded_by="XM_077646235.1:203..949"
                     /db_xref="GeneID:144113258"
ORIGIN      
        1 msqgmdhiav adnvssqade iealsaiyqe dwkvedaakr vykielcsea sgdklsfqvt
       61 lppeypseap pfyeisapwm sqerkaklla dlddhfynhm gesiiydwie iarkavradp
      121 verrsacasd detpevvesf hllamqpdip sppvdfrgsl nvfhgdiled rrsvfqahla
      181 evhtvreveq vldelktnkk iaeathniwa yrilqteprt vlckdseddg etyagnrllh
      241 lldvchrs
//
LOCUS       XP_077502362             366 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113260 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502362
VERSION     XP_077502362.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646236.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..366
                     /product="uncharacterized protein LOC144113260 isoform X1"
                     /calculated_mol_wt=40881
     Region          <1..38
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            12..17
                     /site_type="other"
                     /note="Walker B"
                     /db_xref="CDD:213179"
     Region          <73..>122
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            order(89..93,95..99)
                     /site_type="other"
                     /note="ABC transporter signature motif"
                     /db_xref="CDD:213179"
     Site            114..119
                     /site_type="other"
                     /note="Walker B"
                     /db_xref="CDD:213179"
     Region          <210..>258
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            226..235
                     /site_type="other"
                     /note="ABC transporter signature motif"
                     /db_xref="CDD:213179"
     Site            250..255
                     /site_type="other"
                     /note="Walker B"
                     /db_xref="CDD:213179"
     CDS             1..366
                     /gene="LOC144113260"
                     /coded_by="XM_077646236.1:493..1593"
                     /db_xref="GeneID:144113260"
ORIGIN      
        1 mvalkeqttv pfrvvdnine efralpktak evndqllfee qtlscmlpvd asvslsrspd
       61 adqydkygvs irvkfrdeap laelsaihqs ggearsvatv lylmalqeqt tvpfrlvdei
      121 nqvereylqc ratvsrleke vagsehqlsl traevaetga rwlhdveell qrvnasfghf
      181 fcelgcvgev spsrspdadq ydkygvsirv kfrdeaplpe lsaihqsgge rsvatmlylm
      241 alqeqttvpf rvvdeinqve reylqcratv srlekevags ehqlsltrae vaetgarwlh
      301 dveellqrvn asfghffcel gcvgellpdl pyseddtmif lmlldspite didvlagapv
      361 gllrdt
//
LOCUS       XP_077502363             366 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113260 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502363
VERSION     XP_077502363.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646237.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..366
                     /product="uncharacterized protein LOC144113260 isoform X1"
                     /calculated_mol_wt=40881
     Region          <1..38
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            12..17
                     /site_type="other"
                     /note="Walker B"
                     /db_xref="CDD:213179"
     Region          <73..>122
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            order(89..93,95..99)
                     /site_type="other"
                     /note="ABC transporter signature motif"
                     /db_xref="CDD:213179"
     Site            114..119
                     /site_type="other"
                     /note="Walker B"
                     /db_xref="CDD:213179"
     Region          <210..>258
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            226..235
                     /site_type="other"
                     /note="ABC transporter signature motif"
                     /db_xref="CDD:213179"
     Site            250..255
                     /site_type="other"
                     /note="Walker B"
                     /db_xref="CDD:213179"
     CDS             1..366
                     /gene="LOC144113260"
                     /coded_by="XM_077646237.1:406..1506"
                     /db_xref="GeneID:144113260"
ORIGIN      
        1 mvalkeqttv pfrvvdnine efralpktak evndqllfee qtlscmlpvd asvslsrspd
       61 adqydkygvs irvkfrdeap laelsaihqs ggearsvatv lylmalqeqt tvpfrlvdei
      121 nqvereylqc ratvsrleke vagsehqlsl traevaetga rwlhdveell qrvnasfghf
      181 fcelgcvgev spsrspdadq ydkygvsirv kfrdeaplpe lsaihqsgge rsvatmlylm
      241 alqeqttvpf rvvdeinqve reylqcratv srlekevags ehqlsltrae vaetgarwlh
      301 dveellqrvn asfghffcel gcvgellpdl pyseddtmif lmlldspite didvlagapv
      361 gllrdt
//
LOCUS       XP_077502364             366 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113260 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502364
VERSION     XP_077502364.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646238.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..366
                     /product="uncharacterized protein LOC144113260 isoform X1"
                     /calculated_mol_wt=40881
     Region          <1..38
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            12..17
                     /site_type="other"
                     /note="Walker B"
                     /db_xref="CDD:213179"
     Region          <73..>122
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            order(89..93,95..99)
                     /site_type="other"
                     /note="ABC transporter signature motif"
                     /db_xref="CDD:213179"
     Site            114..119
                     /site_type="other"
                     /note="Walker B"
                     /db_xref="CDD:213179"
     Region          <210..>258
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            226..235
                     /site_type="other"
                     /note="ABC transporter signature motif"
                     /db_xref="CDD:213179"
     Site            250..255
                     /site_type="other"
                     /note="Walker B"
                     /db_xref="CDD:213179"
     CDS             1..366
                     /gene="LOC144113260"
                     /coded_by="XM_077646238.1:226..1326"
                     /db_xref="GeneID:144113260"
ORIGIN      
        1 mvalkeqttv pfrvvdnine efralpktak evndqllfee qtlscmlpvd asvslsrspd
       61 adqydkygvs irvkfrdeap laelsaihqs ggearsvatv lylmalqeqt tvpfrlvdei
      121 nqvereylqc ratvsrleke vagsehqlsl traevaetga rwlhdveell qrvnasfghf
      181 fcelgcvgev spsrspdadq ydkygvsirv kfrdeaplpe lsaihqsgge rsvatmlylm
      241 alqeqttvpf rvvdeinqve reylqcratv srlekevags ehqlsltrae vaetgarwlh
      301 dveellqrvn asfghffcel gcvgellpdl pyseddtmif lmlldspite didvlagapv
      361 gllrdt
//
LOCUS       XP_077502365             366 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113260 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502365
VERSION     XP_077502365.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646239.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..366
                     /product="uncharacterized protein LOC144113260 isoform X1"
                     /calculated_mol_wt=40881
     Region          <1..38
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            12..17
                     /site_type="other"
                     /note="Walker B"
                     /db_xref="CDD:213179"
     Region          <73..>122
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            order(89..93,95..99)
                     /site_type="other"
                     /note="ABC transporter signature motif"
                     /db_xref="CDD:213179"
     Site            114..119
                     /site_type="other"
                     /note="Walker B"
                     /db_xref="CDD:213179"
     Region          <210..>258
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            226..235
                     /site_type="other"
                     /note="ABC transporter signature motif"
                     /db_xref="CDD:213179"
     Site            250..255
                     /site_type="other"
                     /note="Walker B"
                     /db_xref="CDD:213179"
     CDS             1..366
                     /gene="LOC144113260"
                     /coded_by="XM_077646239.1:240..1340"
                     /db_xref="GeneID:144113260"
ORIGIN      
        1 mvalkeqttv pfrvvdnine efralpktak evndqllfee qtlscmlpvd asvslsrspd
       61 adqydkygvs irvkfrdeap laelsaihqs ggearsvatv lylmalqeqt tvpfrlvdei
      121 nqvereylqc ratvsrleke vagsehqlsl traevaetga rwlhdveell qrvnasfghf
      181 fcelgcvgev spsrspdadq ydkygvsirv kfrdeaplpe lsaihqsgge rsvatmlylm
      241 alqeqttvpf rvvdeinqve reylqcratv srlekevags ehqlsltrae vaetgarwlh
      301 dveellqrvn asfghffcel gcvgellpdl pyseddtmif lmlldspite didvlagapv
      361 gllrdt
//
LOCUS       XP_077502366             354 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113260 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502366
VERSION     XP_077502366.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646240.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..354
                     /product="uncharacterized protein LOC144113260 isoform X2"
                     /calculated_mol_wt=39477
     Region          <61..>110
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            order(77..81,83..87)
                     /site_type="other"
                     /note="ABC transporter signature motif"
                     /db_xref="CDD:213179"
     Site            102..107
                     /site_type="other"
                     /note="Walker B"
                     /db_xref="CDD:213179"
     Region          <198..>246
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            214..223
                     /site_type="other"
                     /note="ABC transporter signature motif"
                     /db_xref="CDD:213179"
     Site            238..243
                     /site_type="other"
                     /note="Walker B"
                     /db_xref="CDD:213179"
     CDS             1..354
                     /gene="LOC144113260"
                     /coded_by="XM_077646240.1:638..1702"
                     /db_xref="GeneID:144113260"
ORIGIN      
        1 mqgtthpqef ralpktakev ndqllfeeqt lscmlpvdas vslsrspdad qydkygvsir
       61 vkfrdeapla elsaihqsgg earsvatvly lmalqeqttv pfrlvdeinq vereylqcra
      121 tvsrlekeva gsehqlsltr aevaetgarw lhdveellqr vnasfghffc elgcvgevsp
      181 srspdadqyd kygvsirvkf rdeaplpels aihqsggers vatmlylmal qeqttvpfrv
      241 vdeinqvere ylqcratvsr lekevagseh qlsltraeva etgarwlhdv eellqrvnas
      301 fghffcelgc vgellpdlpy seddtmiflm lldspitedi dvlagapvgl lrdt
//
LOCUS       XP_077502367             299 aa            linear   INV 25-NOV-2025
DEFINITION  structural maintenance of chromosomes protein 5-like isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077502367
VERSION     XP_077502367.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646241.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..299
                     /product="structural maintenance of chromosomes protein
                     5-like isoform X3"
                     /calculated_mol_wt=33308
     Region          <1..38
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            12..17
                     /site_type="other"
                     /note="Walker B"
                     /db_xref="CDD:213179"
     Region          <73..>122
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            order(89..93,95..99)
                     /site_type="other"
                     /note="ABC transporter signature motif"
                     /db_xref="CDD:213179"
     Site            114..119
                     /site_type="other"
                     /note="Walker B"
                     /db_xref="CDD:213179"
     Region          <210..275
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            226..235
                     /site_type="other"
                     /note="ABC transporter signature motif"
                     /db_xref="CDD:213179"
     Site            250..255
                     /site_type="other"
                     /note="Walker B"
                     /db_xref="CDD:213179"
     CDS             1..299
                     /gene="LOC144113260"
                     /coded_by="XM_077646241.1:493..1392"
                     /db_xref="GeneID:144113260"
ORIGIN      
        1 mvalkeqttv pfrvvdnine efralpktak evndqllfee qtlscmlpvd asvslsrspd
       61 adqydkygvs irvkfrdeap laelsaihqs ggearsvatv lylmalqeqt tvpfrlvdei
      121 nqvereylqc ratvsrleke vagsehqlsl traevaetga rwlhdveell qrvnasfghf
      181 fcelgcvgev spsrspdadq ydkygvsirv kfrdeaplpe lsaihqsgge rsvatmlylm
      241 alqeqttvpf rvvdeinqll pdlpyseddt miflmlldsp itedidvlag apvgllrdt
//
LOCUS       XP_077502368             296 aa            linear   INV 25-NOV-2025
DEFINITION  structural maintenance of chromosomes protein 5-like isoform X4
            [Amblyomma americanum].
ACCESSION   XP_077502368
VERSION     XP_077502368.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646242.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..296
                     /product="structural maintenance of chromosomes protein
                     5-like isoform X4"
                     /calculated_mol_wt=33118
     Region          <1..38
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            12..17
                     /site_type="other"
                     /note="Walker B"
                     /db_xref="CDD:213179"
     Region          <140..>188
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            156..165
                     /site_type="other"
                     /note="ABC transporter signature motif"
                     /db_xref="CDD:213179"
     Site            180..185
                     /site_type="other"
                     /note="Walker B"
                     /db_xref="CDD:213179"
     CDS             1..296
                     /gene="LOC144113260"
                     /coded_by="XM_077646242.1:493..1383"
                     /db_xref="GeneID:144113260"
ORIGIN      
        1 mvalkeqttv pfrvvdnine efralpktak evndqllfee qtlscmlpvd asvereylqc
       61 ratvsrleke vagsehqlsl traevaetga rwlhdveell qrvnasfghf fcelgcvgev
      121 spsrspdadq ydkygvsirv kfrdeaplpe lsaihqsgge rsvatmlylm alqeqttvpf
      181 rvvdeinqve reylqcratv srlekevags ehqlsltrae vaetgarwlh dveellqrvn
      241 asfghffcel gcvgellpdl pyseddtmif lmlldspite didvlagapv gllrdt
//
LOCUS       XP_077502369             284 aa            linear   INV 25-NOV-2025
DEFINITION  structural maintenance of chromosomes protein 5-like isoform X5
            [Amblyomma americanum].
ACCESSION   XP_077502369
VERSION     XP_077502369.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646243.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..284
                     /product="structural maintenance of chromosomes protein
                     5-like isoform X5"
                     /calculated_mol_wt=31713
     Region          <128..>176
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            144..153
                     /site_type="other"
                     /note="ABC transporter signature motif"
                     /db_xref="CDD:213179"
     Site            168..173
                     /site_type="other"
                     /note="Walker B"
                     /db_xref="CDD:213179"
     CDS             1..284
                     /gene="LOC144113260"
                     /coded_by="XM_077646243.1:638..1492"
                     /db_xref="GeneID:144113260"
ORIGIN      
        1 mqgtthpqef ralpktakev ndqllfeeqt lscmlpvdas vereylqcra tvsrlekeva
       61 gsehqlsltr aevaetgarw lhdveellqr vnasfghffc elgcvgevsp srspdadqyd
      121 kygvsirvkf rdeaplpels aihqsggers vatmlylmal qeqttvpfrv vdeinqvere
      181 ylqcratvsr lekevagseh qlsltraeva etgarwlhdv eellqrvnas fghffcelgc
      241 vgellpdlpy seddtmiflm lldspitedi dvlagapvgl lrdt
//
LOCUS       XP_077502370             703 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113262 [Amblyomma americanum].
ACCESSION   XP_077502370
VERSION     XP_077502370.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646244.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..703
                     /product="uncharacterized protein LOC144113262"
                     /calculated_mol_wt=79842
     Region          191..280
                     /region_name="SCAN"
                     /note="SCAN domain; pfam02023"
                     /db_xref="CDD:460417"
     Region          348..363
                     /region_name="zf-CCHC"
                     /note="Zinc knuckle; pfam00098"
                     /db_xref="CDD:395050"
     CDS             1..703
                     /gene="LOC144113262"
                     /coded_by="XM_077646244.1:842..2953"
                     /db_xref="GeneID:144113262"
ORIGIN      
        1 mdlmkllrsd llslceelgv dveekmkksh ictllletan eetvsdtwel lkaerkreet
       61 erkkeenerq rlaaereere lrrlqlandq kklecessrg mapervsqae sfpmdrlmqp
      121 ykigedlgyy lvnfertcek rgyaketwpq kllmvlpcea ativarldak daddyekvka
      181 sllrkyrlsa eefrqrfrga skrssesyae fafslkanlt ewlkgaeayg tkdkvvecvg
      241 leqfynsipe avklwvqdre nvstvekaae ladeyatrrk lssessesek kafglrkrfi
      301 pknrsqfrnr etvegtkqap ektedrakqa gaqkaltkqf earkpfrcfn cqetghiaar
      361 ctktrlafsy andsdenlel lrpyihelqv ngkscrvlrd sgatmdivhp syvkeeditg
      421 evawirqvle nqsvclpmar vvisgpfgel vteaavssav slqypylfsn ksnqllcdrg
      481 qklaegtvka ltrsksrela sklrysqese atgeregipe iaepskenav raheqqqepv
      541 tegspidasl qeacspdsml lptslcmdrl lscassrstg atrrlsepss pptfrrgppi
      601 vsdewaahqc vpglldssgv nmnlthqtvn hsqffvdpva gtrtqsiess wqkarlvrsg
      661 rkltpqltet ylgwlwwqst ngrrrchdpf lrlmeaiakr ysm
//
LOCUS       XP_077502371             241 aa            linear   INV 25-NOV-2025
DEFINITION  NPC intracellular cholesterol transporter 1-like [Amblyomma
            americanum].
ACCESSION   XP_077502371
VERSION     XP_077502371.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646245.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..241
                     /product="NPC intracellular cholesterol transporter
                     1-like"
                     /calculated_mol_wt=26121
     Region          35..>216
                     /region_name="NPC1_N"
                     /note="Niemann-Pick C1 N terminus; pfam16414"
                     /db_xref="CDD:465110"
     CDS             1..241
                     /gene="LOC144113263"
                     /coded_by="XM_077646245.1:15..740"
                     /db_xref="GeneID:144113263"
ORIGIN      
        1 mhrsgiprra pstmssqwlf alavgiafvs saraqcisyg ycgtdddsgk plpcsvsrep
       61 vpikknvieg acpaligssg esvpaccdvk qaeafksefk tlfnlgvgkt sacflnfqnl
      121 vcqafcsprq seffaingtv ekgnehpsat dsvyavskkf aervyntckd vrtyvfgikl
      181 mrymcgkhgn sncnaerlle fvgsiwsegg yspikirhvi tdspitvggk klepfspehl
      241 h
//
LOCUS       XP_077502372             590 aa            linear   INV 25-NOV-2025
DEFINITION  poly [ADP-ribose] polymerase 2-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502372
VERSION     XP_077502372.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646246.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..590
                     /product="poly [ADP-ribose] polymerase 2-like isoform X1"
                     /calculated_mol_wt=66497
     Region          <122..586
                     /region_name="PLN03124"
                     /note="poly [ADP-ribose] polymerase; Provisional"
                     /db_xref="CDD:215591"
     CDS             1..590
                     /gene="LOC144113264"
                     /coded_by="XM_077646246.1:147..1919"
                     /db_xref="GeneID:144113264"
ORIGIN      
        1 mpkrlapppa aaavgrkkkt cvrkkeveev ividsssedd dgnasvvcie sdctsddsee
       61 ftsfesiacs sltgekptek epqerpaved vkkpplevvp rvdplctckg plfvysnsyg
      121 fynvmltqtc leknvnkfyt mqllvkphdc syylwcrwgr vgekgvfsli sygddlfkaq
      181 vlfekrflqk tknewskrcs ftkysgkyym lkmdyteegt eseniekrvv dlandtvksr
      241 lepsvkalmt fisdvkelqk ividmkfdvv kmplgkltke qiregykalc kveacinkns
      301 sqkelmdacs efytciphsf prlqrpeliq tneevrmmmr lleafediet alsllkediq
      361 nrythpldnf ysslgcdise ldgsdsdlpl ilqavantqg ethtsyrlvv qnvfkvhkks
      421 qkfssvgnhr mlwhgsqasn fagilsqglr iappkvpsng hmfgkgiyfa dcvsksanyc
      481 tsgrrskegl lllcevslgs vvtrmnsqrn adsqlpcdrh svqglgkysp dpegnvvtks
      541 gavlacgrll ldqdmksalq yneyivysss qakiaylvrv kfdyelyslr
//
LOCUS       XP_077502374             587 aa            linear   INV 25-NOV-2025
DEFINITION  poly [ADP-ribose] polymerase 2-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502374
VERSION     XP_077502374.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646248.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..587
                     /product="poly [ADP-ribose] polymerase 2-like isoform X2"
                     /calculated_mol_wt=66328
     Region          15..583
                     /region_name="PLN03124"
                     /note="poly [ADP-ribose] polymerase; Provisional"
                     /db_xref="CDD:215591"
     CDS             1..587
                     /gene="LOC144113264"
                     /coded_by="XM_077646248.1:496..2259"
                     /db_xref="GeneID:144113264"
ORIGIN      
        1 myivaafyfi pgfplptssr kkeveevivi dsssedddgn asvvciesdc tsddseefts
       61 fesiacsslt gekptekepq erpavedvkk pplevvprvd plctckgplf vysnsygfyn
      121 vmltqtclek nvnkfytmql lvkphdcsyy lwcrwgrvge kgvfslisyg ddlfkaqvlf
      181 ekrflqktkn ewskrcsftk ysgkyymlkm dyteegtese niekrvvdla ndtvksrlep
      241 svkalmtfis dvkelqkivi dmkfdvvkmp lgkltkeqir egykalckve acinknssqk
      301 elmdacsefy tciphsfprl qrpeliqtne evrmmmrlle afedietals llkediqnry
      361 thpldnfyss lgcdiseldg sdsdlplilq avantqgeth tsyrlvvqnv fkvhkksqkf
      421 ssvgnhrmlw hgsqasnfag ilsqglriap pkvpsnghmf gkgiyfadcv sksanyctsg
      481 rrskegllll cevslgsvvt rmnsqrnads qlpcdrhsvq glgkyspdpe gnvvtksgav
      541 lacgrllldq dmksalqyne yivysssqak iaylvrvkfd yelyslr
//
LOCUS       XP_077502375             586 aa            linear   INV 25-NOV-2025
DEFINITION  poly [ADP-ribose] polymerase 2-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502375
VERSION     XP_077502375.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646249.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..586
                     /product="poly [ADP-ribose] polymerase 2-like isoform X3"
                     /calculated_mol_wt=66226
     Region          <118..582
                     /region_name="PLN03124"
                     /note="poly [ADP-ribose] polymerase; Provisional"
                     /db_xref="CDD:215591"
     CDS             1..586
                     /gene="LOC144113264"
                     /coded_by="XM_077646249.1:496..2256"
                     /db_xref="GeneID:144113264"
ORIGIN      
        1 myivaafyfi pgfplptssr kkeveevivi dsssedddgn asvvciesdc tsddseefts
       61 fesiacsslt gekpkkepqe rpavedvkkp plevvprvdp lctckgplfv ysnsygfynv
      121 mltqtclekn vnkfytmqll vkphdcsyyl wcrwgrvgek gvfslisygd dlfkaqvlfe
      181 krflqktkne wskrcsftky sgkyymlkmd yteegtesen iekrvvdlan dtvksrleps
      241 vkalmtfisd vkelqkivid mkfdvvkmpl gkltkeqire gykalckvea cinknssqke
      301 lmdacsefyt ciphsfprlq rpeliqtnee vrmmmrllea fedietalsl lkediqnryt
      361 hpldnfyssl gcdiseldgs dsdlplilqa vantqgetht syrlvvqnvf kvhkksqkfs
      421 svgnhrmlwh gsqasnfagi lsqglriapp kvpsnghmfg kgiyfadcvs ksanyctsgr
      481 rskegllllc evslgsvvtr mnsqrnadsq lpcdrhsvqg lgkyspdpeg nvvtksgavl
      541 acgrllldqd mksalqyney ivysssqaki aylvrvkfdy elyslr
//
LOCUS       XP_077502376             118 aa            linear   INV 25-NOV-2025
DEFINITION  protein C10 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502376
VERSION     XP_077502376.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646250.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..118
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..118
                     /product="protein C10 isoform X1"
                     /calculated_mol_wt=13138
     Region          11..113
                     /region_name="P_C10"
                     /note="Protein C10; pfam14974"
                     /db_xref="CDD:464416"
     CDS             1..118
                     /gene="LOC144113265"
                     /coded_by="XM_077646250.1:107..463"
                     /db_xref="GeneID:144113265"
ORIGIN      
        1 msanlafken fdsekakgal edilaafdqp enfarlqdak dlagndmlkh mqvvfplltq
       61 iemgviesyg ftrdgegvlq faqiikqmer ehpeiarlna elrahfippm vppslpdn
//
LOCUS       XP_077502377             106 aa            linear   INV 25-NOV-2025
DEFINITION  protein C10 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502377
VERSION     XP_077502377.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646251.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..106
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..106
                     /product="protein C10 isoform X2"
                     /calculated_mol_wt=11690
     Region          11..>78
                     /region_name="P_C10"
                     /note="Protein C10; pfam14974"
                     /db_xref="CDD:464416"
     CDS             1..106
                     /gene="LOC144113265"
                     /coded_by="XM_077646251.1:117..437"
                     /db_xref="GeneID:144113265"
ORIGIN      
        1 msanlafken fdsekakgal edilaafdqp enfarlqdak dlagndmlkh mqvvfplltq
       61 iemgviesyg ftrdgealsp kcvlpqcasi imfhscfhgc lhrprc
//
LOCUS       XP_077502378             869 aa            linear   INV 25-NOV-2025
DEFINITION  G patch domain-containing protein 1 [Amblyomma americanum].
ACCESSION   XP_077502378
VERSION     XP_077502378.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646252.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..869
                     /product="G patch domain-containing protein 1"
                     /calculated_mol_wt=96412
     Region          28..107
                     /region_name="DUF1604"
                     /note="Protein of unknown function (DUF1604); pfam07713"
                     /db_xref="CDD:462241"
     Region          144..177
                     /region_name="G-patch"
                     /note="G-patch domain; pfam01585"
                     /db_xref="CDD:396249"
     CDS             1..869
                     /gene="LOC144113266"
                     /coded_by="XM_077646252.1:145..2754"
                     /db_xref="GeneID:144113266"
ORIGIN      
        1 msgeyaflgt pleeldldev prkkfqpdlt vrdaqgrrrf hgaftggfsa gffntvgtke
       61 gwapstykss rtnksenvas rpedfmdeed lgefgiapkk vqvqddfvdf aeqrkrlrdp
      121 prmhdgpipg spplknlfkp vshsigvkll nimgwrqgqg igervkrgkp kqaeerptvk
      181 vygcalppgt esseeedvfa aehtfapadv ddvlyvpkdn qhgigyvpld khsvlsapid
      241 idqptttkcs lekgkrklai tgqafgvgaf eeddsdiyar edmsqysfev adepddkprr
      301 tlpaieggrs kplcdltktl egfhlakepq avlkfypppk ippdfkpihe vprrerrrfe
      361 epksdlckem ktehtgsifd lvspadrerl esiknagsae avkgignssl sqpsqpvsqp
      421 tssskapvpv pssepgrpmk pfardeakqr rfeqylslln snrlevfpam qplnmtewek
      481 ererkefqra aaicqpnqgl esrfvstrtl kdddsidvpv dkaeetpdat kaaqlgmfgr
      541 lthnefewhp vdllcrrfnv pnpypncgkv glatvtvdkh avfnfltpsn keeepskapd
      601 dsegldqvse patsnpglsr avesqpdkee vqdssnepee erppleffns ifsesseded
      661 ekprdgraas geaatpkeav agvveqlgsp vevvkrpqrp algvfanldf eklnrrppae
      721 plqdvhssaa sstvassssd krpspsketp ddniygpklp pgdpfsvdte apgpairhgt
      781 sslhhshrhk kkdhksksgk khknklkskl kkkkhkkhsk qettkkdsgh kssetgessd
      841 ssgeestkyq kepsykdima rleklkklm
//
LOCUS       XP_077502379             449 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113271 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502379
VERSION     XP_077502379.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646253.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..449
                     /product="uncharacterized protein LOC144113271 isoform X1"
                     /calculated_mol_wt=49125
     Region          <17..243
                     /region_name="PRK02224"
                     /note="DNA double-strand break repair Rad50 ATPase"
                     /db_xref="CDD:179385"
     CDS             1..449
                     /gene="LOC144113271"
                     /coded_by="XM_077646253.1:328..1677"
                     /db_xref="GeneID:144113271"
ORIGIN      
        1 mgervydhws rsqgvtsdms delarkkeev krlsaesarm dsvireqmae ieglcsgitn
       61 feqlaadsad elarvqaeae slkeslaaaq meaeereeaa llllekdtqi aqlegelqek
      121 reelaslvqe kaavdeserl recnqnwkei vssiskkleq eknwresmei qflktskgei
      181 aqkpsehsrq alakletenk hlkakvsklq ekvcrltnei aetrdaeark reriehlyre
      241 akeehqfmrr astaaragvt pgrqastavq rgsrpvpaap qdvpkcmptq ecpftssflh
      301 tphkpstsva qapsliapaf aeapasstfr grvvvhepes ssstspglev avmpaepsqe
      361 stssemslse lasiieaipv kpkpptgnag gkscstksha vaatkaskhl qkaktpvkas
      421 pckrkhpcta savpasskks klekkedss
//
LOCUS       XP_077502380             449 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113271 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502380
VERSION     XP_077502380.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646254.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..449
                     /product="uncharacterized protein LOC144113271 isoform X1"
                     /calculated_mol_wt=49125
     Region          <17..243
                     /region_name="PRK02224"
                     /note="DNA double-strand break repair Rad50 ATPase"
                     /db_xref="CDD:179385"
     CDS             1..449
                     /gene="LOC144113271"
                     /coded_by="XM_077646254.1:313..1662"
                     /db_xref="GeneID:144113271"
ORIGIN      
        1 mgervydhws rsqgvtsdms delarkkeev krlsaesarm dsvireqmae ieglcsgitn
       61 feqlaadsad elarvqaeae slkeslaaaq meaeereeaa llllekdtqi aqlegelqek
      121 reelaslvqe kaavdeserl recnqnwkei vssiskkleq eknwresmei qflktskgei
      181 aqkpsehsrq alakletenk hlkakvsklq ekvcrltnei aetrdaeark reriehlyre
      241 akeehqfmrr astaaragvt pgrqastavq rgsrpvpaap qdvpkcmptq ecpftssflh
      301 tphkpstsva qapsliapaf aeapasstfr grvvvhepes ssstspglev avmpaepsqe
      361 stssemslse lasiieaipv kpkpptgnag gkscstksha vaatkaskhl qkaktpvkas
      421 pckrkhpcta savpasskks klekkedss
//
LOCUS       XP_077502381             449 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113271 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502381
VERSION     XP_077502381.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646255.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..449
                     /product="uncharacterized protein LOC144113271 isoform X1"
                     /calculated_mol_wt=49125
     Region          <17..243
                     /region_name="PRK02224"
                     /note="DNA double-strand break repair Rad50 ATPase"
                     /db_xref="CDD:179385"
     CDS             1..449
                     /gene="LOC144113271"
                     /coded_by="XM_077646255.1:209..1558"
                     /db_xref="GeneID:144113271"
ORIGIN      
        1 mgervydhws rsqgvtsdms delarkkeev krlsaesarm dsvireqmae ieglcsgitn
       61 feqlaadsad elarvqaeae slkeslaaaq meaeereeaa llllekdtqi aqlegelqek
      121 reelaslvqe kaavdeserl recnqnwkei vssiskkleq eknwresmei qflktskgei
      181 aqkpsehsrq alakletenk hlkakvsklq ekvcrltnei aetrdaeark reriehlyre
      241 akeehqfmrr astaaragvt pgrqastavq rgsrpvpaap qdvpkcmptq ecpftssflh
      301 tphkpstsva qapsliapaf aeapasstfr grvvvhepes ssstspglev avmpaepsqe
      361 stssemslse lasiieaipv kpkpptgnag gkscstksha vaatkaskhl qkaktpvkas
      421 pckrkhpcta savpasskks klekkedss
//
LOCUS       XP_077502382             434 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113271 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502382
VERSION     XP_077502382.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646256.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..434
                     /product="uncharacterized protein LOC144113271 isoform X2"
                     /calculated_mol_wt=47447
     Region          <2..>256
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..434
                     /gene="LOC144113271"
                     /coded_by="XM_077646256.1:328..1632"
                     /db_xref="GeneID:144113271"
ORIGIN      
        1 mgervydhws rsqgvtsdms delarkkeev krlsaesarm dsvireqmae ieglcsgitn
       61 feqlaadsad elarvqaeae slkeslaaaq meaeereeaa llllekdtqi aqlegelqek
      121 reelaslvqe kaavdeserl recnqnwkei vssiskkleq eknwresmei qflktskgei
      181 aqknkhlkak vsklqekvcr ltneiaetrd aearkrerie hlyreakeeh qfmrrastaa
      241 ragvtpgrqa stavqrgsrp vpaapqdvpk cmptqecpft ssflhtphkp stsvaqapsl
      301 iapafaeapa sstfrgrvvv hepessssts pglevavmpa epsqestsse mslselasii
      361 eaipvkpkpp tgnaggkscs tkshavaatk askhlqkakt pvkaspckrk hpctasavpa
      421 sskksklekk edss
//
LOCUS       XP_077502383             402 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113271 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502383
VERSION     XP_077502383.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646257.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..402
                     /product="uncharacterized protein LOC144113271 isoform X3"
                     /calculated_mol_wt=43657
     Region          59..>212
                     /region_name="YhaN"
                     /note="Uncharacterized conserved protein YhaN, contains
                     AAA domain [Function unknown]; COG4717"
                     /db_xref="CDD:443752"
     CDS             1..402
                     /gene="LOC144113271"
                     /coded_by="XM_077646257.1:1944..3152"
                     /db_xref="GeneID:144113271"
ORIGIN      
        1 maeieglcsg itnfeqlaad sadelarvqa eaeslkesla aaqmeaeere eaallllekd
       61 tqiaqlegel qekreelasl vqekaavdes erlrecnqnw keivssiskk leqeknwres
      121 meiqflktsk geiaqkpseh srqalaklet enkhlkakvs klqekvcrlt neiaetrdae
      181 arkreriehl yreakeehqf mrrastaara gvtpgrqast avqrgsrpvp aapqdvpkcm
      241 ptqecpftss flhtphkpst svaqapslia pafaeapass tfrgrvvvhe pesssstspg
      301 levavmpaep sqestssems lselasiiea ipvkpkpptg naggkscstk shavaatkas
      361 khlqkaktpv kaspckrkhp ctasavpass kksklekked ss
//
LOCUS       XP_077502384             449 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113272 [Amblyomma americanum].
ACCESSION   XP_077502384
VERSION     XP_077502384.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646258.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..449
                     /product="uncharacterized protein LOC144113272"
                     /calculated_mol_wt=49081
     Region          <17..243
                     /region_name="PRK02224"
                     /note="DNA double-strand break repair Rad50 ATPase"
                     /db_xref="CDD:179385"
     CDS             1..449
                     /gene="LOC144113272"
                     /coded_by="XM_077646258.1:308..1657"
                     /db_xref="GeneID:144113272"
ORIGIN      
        1 mgervydhws rsqgvtsdms delarkkeev krlsaesarm dsvireqmae ieglcsgitn
       61 feqlaadsad elarvqaeae slkeslaaaq meaeereeaa llllekdtqi aqlegelqek
      121 reelaslvqe kaavdeserl recnqnwkei vssiskkleq eknwresmei qflktskgei
      181 aqkpsehsrq alakletenk hlkakvsklq ekvcrltnei aetrdaeark reriehlyre
      241 akeehqfmrr astaaragvt pglqastavq rgsrpvpaap qdvpkcmptq ecpftssflh
      301 tphkpstsva qapsliapaf aeapasstfr grvvvhepes ssstspglev avmpaepsqe
      361 stssemslse lasiieaipv kpkpptgnag gkscstksha vaatkaskhl qkaktpvkas
      421 pckrkhpcta savpasskks klekkedss
//
LOCUS       XP_077502385             309 aa            linear   INV 25-NOV-2025
DEFINITION  sulfotransferase ssu-1-like [Amblyomma americanum].
ACCESSION   XP_077502385
VERSION     XP_077502385.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646259.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..309
                     /product="sulfotransferase ssu-1-like"
                     /calculated_mol_wt=35668
     Region          36..302
                     /region_name="Sulfotransfer_1"
                     /note="Sulfotransferase domain; pfam00685"
                     /db_xref="CDD:425820"
     CDS             1..309
                     /gene="LOC144113274"
                     /coded_by="XM_077646259.1:101..1030"
                     /db_xref="GeneID:144113274"
ORIGIN      
        1 mpvmrkpsyv dvdgirlpgg fdpvhvreal nfkpnpgdif ivtypkcgth wiqqisqlil
       61 nrgvsatnyl efqmktpfie ftgtkmldai ppprllkthf afdrqpyhrd akyvymvrnp
      121 kdccvsffhh trgipgykfs ngsfddffel firgetdfgd yfdnllswyd hkddpnvffl
      181 tyeqlkadak twvlrlahff gkeyaeplkk dpdllqqvld kssvqymkdh ielsekdlqi
      241 lsegganpal epvrrmfvpq dgrgpsiqfi rkgqvgdwrg hftpdqeakm qarvkektag
      301 tdvmglwkd
//
LOCUS       XP_077502386             309 aa            linear   INV 25-NOV-2025
DEFINITION  sulfotransferase ssu-1-like [Amblyomma americanum].
ACCESSION   XP_077502386
VERSION     XP_077502386.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646260.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..309
                     /product="sulfotransferase ssu-1-like"
                     /calculated_mol_wt=35668
     Region          36..302
                     /region_name="Sulfotransfer_1"
                     /note="Sulfotransferase domain; pfam00685"
                     /db_xref="CDD:425820"
     CDS             1..309
                     /gene="LOC144113274"
                     /coded_by="XM_077646260.1:186..1115"
                     /db_xref="GeneID:144113274"
ORIGIN      
        1 mpvmrkpsyv dvdgirlpgg fdpvhvreal nfkpnpgdif ivtypkcgth wiqqisqlil
       61 nrgvsatnyl efqmktpfie ftgtkmldai ppprllkthf afdrqpyhrd akyvymvrnp
      121 kdccvsffhh trgipgykfs ngsfddffel firgetdfgd yfdnllswyd hkddpnvffl
      181 tyeqlkadak twvlrlahff gkeyaeplkk dpdllqqvld kssvqymkdh ielsekdlqi
      241 lsegganpal epvrrmfvpq dgrgpsiqfi rkgqvgdwrg hftpdqeakm qarvkektag
      301 tdvmglwkd
//
LOCUS       XP_077502387             275 aa            linear   INV 25-NOV-2025
DEFINITION  inositol monophosphatase 2 [Amblyomma americanum].
ACCESSION   XP_077502387
VERSION     XP_077502387.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646261.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..275
                     /product="inositol monophosphatase 2"
                     /calculated_mol_wt=29657
     Region          14..255
                     /region_name="IMPase"
                     /note="inositol monophosphatase and related domains. A
                     family of Mg++ dependent phosphatases, inhibited by
                     lithium, many of which may act on inositol monophosphate
                     substrate. They dephosphorylate inositol phosphate to
                     generate inositol, which may be recycled...; cd01639"
                     /db_xref="CDD:238817"
     Site            order(38,43,49,72..73,92..97,220..221)
                     /site_type="active"
                     /db_xref="CDD:238817"
     Site            order(42,44,98..99,101..106,158..160,162,164..165,
                     174..175,178,181..182,185,189..195,203,207..210)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:238817"
     CDS             1..275
                     /gene="LOC144113275"
                     /coded_by="XM_077646261.1:132..959"
                     /db_xref="GeneID:144113275"
ORIGIN      
        1 mpgnqdiqly fsralelvke agtvvrkalg egkqvdtkse fsdlvtqydk qveqlligkl
       61 reqfpnhrfi geesavagvk neltdnptwi idpidgttnf vhgfpvvavs valavqkeva
      121 lgivynpvqd rmytaikghg afcngsklkv sgqdeiskal iisevgssrd aghmqfvfqn
      181 mhnlmkkaqg lrclgsaald mcsvasgead amyefgihvw diaaaalivt eaggvvmdtq
      241 ggplnlmhrr vlcassqvla ntisqildhv qlesd
//
LOCUS       XP_077502388            1264 aa            linear   INV 25-NOV-2025
DEFINITION  small wing phospholipase C gamma 1 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502388
VERSION     XP_077502388.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646262.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1264
                     /product="small wing phospholipase C gamma 1 isoform X1"
                     /calculated_mol_wt=146565
     Region          28..150
                     /region_name="PH_PLC_gamma"
                     /note="Phospholipase C-gamma (PLC-gamma) pleckstrin
                     homology (PH) domain; cd13362"
                     /db_xref="CDD:270168"
     Site            order(33,35,47,62,124)
                     /site_type="other"
                     /note="putative Rac1 binding site [polypeptide binding]"
                     /db_xref="CDD:270168"
     Site            order(36,38,44,46,48,61..62)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:270168"
     Region          155..296
                     /region_name="EFh_PI-PLCgamma"
                     /note="EF-hand motif found in phosphoinositide
                     phospholipase C gamma isozymes (PI-PLC-gamma); cd16201"
                     /db_xref="CDD:320031"
     Region          155..183
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320031"
     Region          190..219
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320031"
     Region          223..252
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320031"
     Region          262..296
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320031"
     Region          308..>470
                     /region_name="PI-PLCc_gamma"
                     /note="Catalytic domain of metazoan
                     phosphoinositide-specific phospholipase C-gamma; cd08592"
                     /db_xref="CDD:176534"
     Site            order(324,369)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:176534"
     Site            order(325,354,356,403)
                     /site_type="other"
                     /note="putative Ca binding site [ion binding]"
                     /db_xref="CDD:176534"
     Region          482..>510
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          541..639
                     /region_name="SH2_N-SH2_PLC_gamma_like"
                     /note="N-terminal Src homology 2 (N-SH2) domain in
                     Phospholipase C gamma; cd10341"
                     /db_xref="CDD:199829"
     Site            order(555,576,578,586,597)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:199829"
     Site            order(589,598,613,636)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:199829"
     Region          653..756
                     /region_name="SH2_C-SH2_PLC_gamma_like"
                     /note="C-terminal Src homology 2 (C-SH2) domain in
                     Phospholipase C gamma; cd09932"
                     /db_xref="CDD:198186"
     Site            order(665,684,705,707)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198186"
     Site            order(706,732)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198186"
     Region          786..838
                     /region_name="SH3_PLCgamma"
                     /note="Src homology 3 domain of Phospholipase C (PLC)
                     gamma; cd11825"
                     /db_xref="CDD:212759"
     Site            order(791,797,799..800,819,831,835..836)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212759"
     Region          828..921
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          941..1052
                     /region_name="PI-PLC-Y"
                     /note="Phosphatidylinositol-specific phospholipase C, Y
                     domain; pfam00387"
                     /db_xref="CDD:459794"
     Region          1071..1193
                     /region_name="C2_PLC_like"
                     /note="C2 domain present in Phosphoinositide-specific
                     phospholipases C (PLC); cd00275"
                     /db_xref="CDD:175974"
     Site            order(1093,1117,1146,1148,1154,1156)
                     /site_type="other"
                     /note="Ca2+ binding pocket [ion binding]"
                     /db_xref="CDD:175974"
     CDS             1..1264
                     /gene="sl"
                     /coded_by="XM_077646262.1:449..4243"
                     /db_xref="GeneID:144113276"
ORIGIN      
        1 maasvhqngs vpvalsclpe ieqvirkleh gtslikfyqk grpekrtfsi kletrqviww
       61 rpvtgrtvee gavdlrevke vrlgrnskif drwpedarkw dpaqcfvify gsafrlktls
      121 cvatsakdce qwihgirylv adtlsasypl qverwlrkef ysmqnprnvi tlkdlkaffp
      181 kvncklstnk lkeyfqevdt nrageigfeg faslyhslih deklfndffk pyadesgkvt
      241 lkafqkflsq eqkgavfdde rvvskfmrdy lqdplrdaqe pyftvpefld ylfskqnelw
      301 dsqhnkvnqd mtrplthywv asshntyltg dqvksessve ayarcltmsc rcieldcwdg
      361 pdgmpyvyhg htlttkirfi dviktikdha fvaseypvil sienhctlpq qrkmanlfqe
      421 vfgemlltqp ieregtkmps peqlkwkvii khkklpegtd dkvivnrsde gsqetdvsna
      481 vkngilyled pvdkewrahf fmltqnrmyy aeeqqmqdee eegdgtelqe qregvpseel
      541 hfgekwfhgk laggrlkaqe llqqhahlgd gtflvresdt fvgdyslsfw rqgkvnhcri
      601 kskqergqtk yylidtvafd tlynlishyq thplrsqefy mylnepvpqp nkhegkewyh
      661 anmtrthaee llkrvkydgt flvrpseked ncfaisfrae nklkhcrikq egrlfvigta
      721 qfeslvelvn yyekhplyrk vklkypined iirrigtdpe dsplhvggpa gtymdpdsfs
      781 gafratvkal ydyraqrdde lsfckhaiit nvvrldggww rgnyggkrqh wfpanfvevl
      841 etqdasddgs gdamplgtlq kgsidiagct vdilqnwpgr gqdsvfrivs ptqlvpieia
      901 apsreemqdw vlkiretaqs andmiqqrre merslrlake lsnliiycrs vqfcperign
      961 ftemssfpet kvekwmspqh csfflkyhrl qfsrvypkgs ridssnydpv hlwncgcqmv
     1021 alnyqtpdrp mqlnqgrflq ngrcgyvlrp dfmhdddydp ydrttlksvh pitlsikvia
     1081 arhlmksgrg ivspfveiei vgadydscry ktgtipdngl npvwneafmf dicnpsvall
     1141 rfvvqdedmf gdpnflgqvt ypvcclrtgy rsiplkneys eelelasllv hlethatkee
     1201 deayvciqrl rdesrelgra leeserrgdm qqaqalrqql tttqeqlrtk reerrlller
     1261 sqpa
//
LOCUS       XP_077502389            1261 aa            linear   INV 25-NOV-2025
DEFINITION  small wing phospholipase C gamma 1 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502389
VERSION     XP_077502389.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646263.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1261
                     /product="small wing phospholipase C gamma 1 isoform X2"
                     /calculated_mol_wt=146191
     Region          28..150
                     /region_name="PH_PLC_gamma"
                     /note="Phospholipase C-gamma (PLC-gamma) pleckstrin
                     homology (PH) domain; cd13362"
                     /db_xref="CDD:270168"
     Site            order(33,35,47,62,124)
                     /site_type="other"
                     /note="putative Rac1 binding site [polypeptide binding]"
                     /db_xref="CDD:270168"
     Site            order(36,38,44,46,48,61..62)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:270168"
     Region          155..296
                     /region_name="EFh_PI-PLCgamma"
                     /note="EF-hand motif found in phosphoinositide
                     phospholipase C gamma isozymes (PI-PLC-gamma); cd16201"
                     /db_xref="CDD:320031"
     Region          155..183
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320031"
     Region          190..219
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320031"
     Region          223..252
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320031"
     Region          262..296
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320031"
     Region          308..>470
                     /region_name="PI-PLCc_gamma"
                     /note="Catalytic domain of metazoan
                     phosphoinositide-specific phospholipase C-gamma; cd08592"
                     /db_xref="CDD:176534"
     Site            order(324,369)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:176534"
     Site            order(325,354,356,403)
                     /site_type="other"
                     /note="putative Ca binding site [ion binding]"
                     /db_xref="CDD:176534"
     Region          482..>510
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          541..639
                     /region_name="SH2_N-SH2_PLC_gamma_like"
                     /note="N-terminal Src homology 2 (N-SH2) domain in
                     Phospholipase C gamma; cd10341"
                     /db_xref="CDD:199829"
     Site            order(555,576,578,586,597)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:199829"
     Site            order(589,598,613,636)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:199829"
     Region          653..756
                     /region_name="SH2_C-SH2_PLC_gamma_like"
                     /note="C-terminal Src homology 2 (C-SH2) domain in
                     Phospholipase C gamma; cd09932"
                     /db_xref="CDD:198186"
     Site            order(665,684,705,707)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198186"
     Site            order(706,732)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198186"
     Region          786..838
                     /region_name="SH3_PLCgamma"
                     /note="Src homology 3 domain of Phospholipase C (PLC)
                     gamma; cd11825"
                     /db_xref="CDD:212759"
     Site            order(791,797,799..800,819,831,835..836)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212759"
     Region          828..921
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          941..1052
                     /region_name="PI-PLC-Y"
                     /note="Phosphatidylinositol-specific phospholipase C, Y
                     domain; pfam00387"
                     /db_xref="CDD:459794"
     Region          1071..1193
                     /region_name="C2_PLC_like"
                     /note="C2 domain present in Phosphoinositide-specific
                     phospholipases C (PLC); cd00275"
                     /db_xref="CDD:175974"
     Site            order(1093,1117,1146,1148,1154,1156)
                     /site_type="other"
                     /note="Ca2+ binding pocket [ion binding]"
                     /db_xref="CDD:175974"
     CDS             1..1261
                     /gene="sl"
                     /coded_by="XM_077646263.1:449..4234"
                     /db_xref="GeneID:144113276"
ORIGIN      
        1 maasvhqngs vpvalsclpe ieqvirkleh gtslikfyqk grpekrtfsi kletrqviww
       61 rpvtgrtvee gavdlrevke vrlgrnskif drwpedarkw dpaqcfvify gsafrlktls
      121 cvatsakdce qwihgirylv adtlsasypl qverwlrkef ysmqnprnvi tlkdlkaffp
      181 kvncklstnk lkeyfqevdt nrageigfeg faslyhslih deklfndffk pyadesgkvt
      241 lkafqkflsq eqkgavfdde rvvskfmrdy lqdplrdaqe pyftvpefld ylfskqnelw
      301 dsqhnkvnqd mtrplthywv asshntyltg dqvksessve ayarcltmsc rcieldcwdg
      361 pdgmpyvyhg htlttkirfi dviktikdha fvaseypvil sienhctlpq qrkmanlfqe
      421 vfgemlltqp ieregtkmps peqlkwkvii khkklpegtd dkvivnrsde gsqetdvsna
      481 vkngilyled pvdkewrahf fmltqnrmyy aeeqqmqdee eegdgtelqe qregvpseel
      541 hfgekwfhgk laggrlkaqe llqqhahlgd gtflvresdt fvgdyslsfw rqgkvnhcri
      601 kskqergqtk yylidtvafd tlynlishyq thplrsqefy mylnepvpqp nkhegkewyh
      661 anmtrthaee llkrvkydgt flvrpseked ncfaisfrae nklkhcrikq egrlfvigta
      721 qfeslvelvn yyekhplyrk vklkypined iirrigtdpe dsplhvggpa gtymdpdsfs
      781 gafratvkal ydyraqrdde lsfckhaiit nvvrldggww rgnyggkrqh wfpanfvevl
      841 etqdasddgs gdamplgtlq kgsidiagct vdilqnwpgr gqdsvfrivs ptqlvpieia
      901 apsreemqdw vlkiretaqs andmiqqrre merslrlake lsnliiycrs vqfcperign
      961 ftemssfpet kvekwmspqh csfflkyhrl qfsrvypkgs ridssnydpv hlwncgcqmv
     1021 alnyqtpdrp mqlnqgrflq ngrcgyvlrp dfmhdddydp ydrttlksvh pitlsikvia
     1081 arhlmksgrg ivspfveiei vgadydscry ktgtipdngl npvwneafmf dicnpsvall
     1141 rfvvqdedmf gdpnflgqvt ypvcclrtgy rsiplkneys eelelasllv hlethatkee
     1201 deayvciqrl rdesrelgra leeserrgdm qqaqalrqql tttqeqlrtk reerrttfta
     1261 l
//
LOCUS       XP_077502390             224 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113278 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502390
VERSION     XP_077502390.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646264.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..224
                     /product="uncharacterized protein LOC144113278 isoform X1"
                     /calculated_mol_wt=25527
     CDS             1..224
                     /gene="LOC144113278"
                     /coded_by="XM_077646264.1:152..826"
                     /db_xref="GeneID:144113278"
ORIGIN      
        1 mlsgcllavg krlpllkpny cqtvlhgtkr eastrwnlfr rrtpeayary dglnptldql
       61 vytsgvhnym llasitatsi aaggvcyvga tyfnlwpeps ngqqpiwsps saqkgafvsv
      121 ismymlacly lirhmpvriy ynkskqmfcf vfqstvpglr rketfkpgal evvpetkyit
      181 irdffgnvrv rngwrrfvvv eerfrfvafy nvmvgfddid vles
//
LOCUS       XP_077502391             223 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113278 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502391
VERSION     XP_077502391.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646265.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..223
                     /product="uncharacterized protein LOC144113278 isoform X2"
                     /calculated_mol_wt=25399
     CDS             1..223
                     /gene="LOC144113278"
                     /coded_by="XM_077646265.1:152..823"
                     /db_xref="GeneID:144113278"
ORIGIN      
        1 mlsgcllavg krlpllkpny ctvlhgtkre astrwnlfrr rtpeayaryd glnptldqlv
       61 ytsgvhnyml lasitatsia aggvcyvgat yfnlwpepsn gqqpiwspss aqkgafvsvi
      121 smymlaclyl irhmpvriyy nkskqmfcfv fqstvpglrr ketfkpgale vvpetkyiti
      181 rdffgnvrvr ngwrrfvvve erfrfvafyn vmvgfddidv les
//
LOCUS       XP_077502392              63 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113278 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502392
VERSION     XP_077502392.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646266.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..63
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..63
                     /product="uncharacterized protein LOC144113278 isoform X3"
                     /calculated_mol_wt=7303
     CDS             1..63
                     /gene="LOC144113278"
                     /coded_by="XM_077646266.1:175..366"
                     /db_xref="GeneID:144113278"
ORIGIN      
        1 mlsgcllavg krlpllkpny cqtvlhgtkr eastrwnlfr rrtpeayary deysawtakk
       61 rnf
//
LOCUS       XP_077502393              62 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113278 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077502393
VERSION     XP_077502393.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646267.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..62
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..62
                     /product="uncharacterized protein LOC144113278 isoform X4"
                     /calculated_mol_wt=7175
     CDS             1..62
                     /gene="LOC144113278"
                     /coded_by="XM_077646267.1:213..401"
                     /db_xref="GeneID:144113278"
ORIGIN      
        1 mlsgcllavg krlpllkpny ctvlhgtkre astrwnlfrr rtpeayaryd eysawtakkr
       61 nf
//
LOCUS       XP_077502394             434 aa            linear   INV 25-NOV-2025
DEFINITION  adenosylhomocysteinase [Amblyomma americanum].
ACCESSION   XP_077502394
VERSION     XP_077502394.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646268.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..434
                     /product="adenosylhomocysteinase"
                     /calculated_mol_wt=47840
     Region          8..433
                     /region_name="AdoHcyase"
                     /note="S-adenosyl-L-homocysteine hydrolase; pfam05221"
                     /db_xref="CDD:461594"
     CDS             1..434
                     /gene="Ahcy"
                     /coded_by="XM_077646268.1:181..1485"
                     /db_xref="GeneID:144113279"
ORIGIN      
        1 msaskpayki adigladfgr keiiiaenem pglmalrrny gpskplkgar iagclhmtiq
       61 tavlietlle lgaevqwssc nifstqdhaa aaiakrgvpv yawkgeteee yiwcieqtlv
      121 fpdgqplnmi lddggdltal vhnkypqylp girglseett tgvhqlykmk segklkipci
      181 nvndsvtksk fdnlygcres ladgikratd imlagkvacv agfgdvgkgc aqslrgfgcr
      241 vlitevdpin alqaamegye vttledaask asifvtatgc rdiirgehfe kmrndaivcn
      301 ighfdieidv kwleqnavek vevkpqvdrf klkngnhiic laqgrlvnlg camghpsfvm
      361 stsftnqvla qielwtkndk ykvdvyflpk kldeevaalh lnhlgvkltk lnkeqssylg
      421 iptegpfkpd yyry
//
LOCUS       XP_077502395              76 aa            linear   INV 25-NOV-2025
DEFINITION  hematopoietic stem/progenitor cell protein 300 [Amblyomma
            americanum].
ACCESSION   XP_077502395
VERSION     XP_077502395.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646269.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..76
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..76
                     /product="hematopoietic stem/progenitor cell protein 300"
                     /calculated_mol_wt=8781
     CDS             1..76
                     /gene="HSPC300"
                     /coded_by="XM_077646269.1:139..369"
                     /db_xref="GeneID:144113281"
ORIGIN      
        1 msvlereaiq kqiqqdwanr eyieiisgsi kkivdfltsf dmscrarlat lnekltsler
       61 rieylearvt kgdtfn
//
LOCUS       XP_077502396             596 aa            linear   INV 25-NOV-2025
DEFINITION  biotin--protein ligase-like [Amblyomma americanum].
ACCESSION   XP_077502396
VERSION     XP_077502396.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646270.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..596
                     /product="biotin--protein ligase-like"
                     /calculated_mol_wt=64688
     Region          353..573
                     /region_name="BirA2"
                     /note="Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme
                     transport and metabolism]; COG0340"
                     /db_xref="CDD:440109"
     CDS             1..596
                     /gene="LOC144113282"
                     /coded_by="XM_077646270.1:417..2207"
                     /db_xref="GeneID:144113282"
ORIGIN      
        1 milhvirrvs frpssyfllp lrgvaspgac dvsgilgssg maphkppnvl iftnedgsrq
       61 prfhavketl gmclspdhyv iyslsehqvk stpwlantkl livactdmss ttrrifteyf
      121 frggnllvma akldlpgvry mehslgpeai atvvheaaed iavlrgqvly qfdqncrvkt
      181 valdqegagl vfelrgssng glallseihl eldpvffgts edvfallkka negrismlrn
      241 ilskhfrlnc slnashklst gniftatkev lqdflsrpqt asgvfvdgnv sfcyvtedka
      301 asmtatvdcl pviipsgdtf fcspafnsai yfsalrthsl grlalfvena tttmtaikal
      361 qsihgsvaia trqlngvgrg gnawlgppgc amftvclqvp lnsplgqksp fvqhlaalav
      421 akavrctegy emvnirvkwp ndiyygshsk mggvlvsstv nrdsitcyvg cginvsnsqp
      481 tlcindivkv apsklgtsqv aspltpekvi artlnemefl vatfqsgcss mvlqeyykyw
      541 lhggqevtlq dygckalirg ldeygyllae csgklyklqp dgnsfdlmnn livtka
//
LOCUS       XP_077502397             384 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger CCCH domain-containing protein 15 [Amblyomma
            americanum].
ACCESSION   XP_077502397
VERSION     XP_077502397.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646271.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..384
                     /product="zinc finger CCCH domain-containing protein 15"
                     /calculated_mol_wt=43796
     Region          1..287
                     /region_name="COG5252"
                     /note="Uncharacterized conserved protein, contains
                     CCCH-type Zn-finger protein [General function prediction
                     only]"
                     /db_xref="CDD:227577"
     CDS             1..384
                     /gene="LOC144113283"
                     /coded_by="XM_077646271.1:105..1259"
                     /db_xref="GeneID:144113283"
ORIGIN      
        1 mppkkkapep skktemkkke kviedktfgl knkkgaktqk fiqqvqhqvk fgnqpakkve
       61 aqvknekkkt eaedlnqllf rpvqkvekga dpksvlcaff kqgtcgkgdk ckfshdlsie
      121 rkaekrdiyt dvrdqdtmdn wdeaklaevv ekkhgeaerr mpktdiickh fldaleqnky
      181 gwfwecpngg qkchyrhavp pgfvlkknkk nadeiktged tltiedliet eraklggnlp
      241 ritlesflaw kkakirekqa seqkerdrkr defkagrsig lsgrdmftfr pelaeddgqd
      301 eedeeaydls rfegeedste vvreitlenl mvesmqgedl eaaaaapiga svstsvpide
      361 klfedddled ledledelgd leik
//
LOCUS       XP_077502398             721 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113284 [Amblyomma americanum].
ACCESSION   XP_077502398
VERSION     XP_077502398.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646272.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..721
                     /product="uncharacterized protein LOC144113284"
                     /calculated_mol_wt=80659
     Region          192..>385
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     Region          193..221
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          222..248
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          249..279
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          280..304
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          338..365
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          <496..589
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     CDS             1..721
                     /gene="LOC144113284"
                     /coded_by="XM_077646272.1:251..2416"
                     /db_xref="GeneID:144113284"
ORIGIN      
        1 mayptnyyhr maphpyplya nlpgfrract asssndcwlc dvlqswnstl rycrlnlree
       61 epgvlclqsl rgtffavede prdkapvhsa afllaylprl hkcirrislh rqhlprqpap
      121 vrlllgadww pqqpecnvqy leissivdae wdiflcglgr vsalkcliie railgnnyvs
      181 kldqllaans gslqklvisa srtqtaeasd rliraisnct hltelsfdch ltrtgvydlg
      241 rlllpceniq klslrdqfga ecrrallgal rsfvetnasl telhykslsl dviqlldalk
      301 fnrtlkhlvl sgykhnqktf grehgaalga alatnsglrs lvishctfse lavedisagl
      361 alntslerfd lshcrvnfdv asalcrvlyt nrtlhmvvld pdgeaisewv rfskqvaaan
      421 cyhrvgmrwd etsmqnivnv lkqpslcppe chidssrfsi tslsqvcsal vsssvksisv
      481 tfidsnlnea rhhvheairk nqsivslslq edrissgaci fvakallenk tvtklslrin
      541 dlytgaaqtl seilsknqtl eklllncnnv dlwcvnviar glkenrtlth fsifderdda
      601 srsiitvdec vdrnlsswnc avqfvlkaci srrraetfes lqgmpcflqr vaaasgksla
      661 eaaamvecak hfirsnylfi tgvvirgicc ypsgqtqvdc lnndcwteit kylrvsdvir
      721 h
//
LOCUS       XP_077502399             721 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113286 [Amblyomma americanum].
ACCESSION   XP_077502399
VERSION     XP_077502399.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646273.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..721
                     /product="uncharacterized protein LOC144113286"
                     /calculated_mol_wt=80611
     Region          192..>385
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     Region          <496..>589
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     CDS             1..721
                     /gene="LOC144113286"
                     /coded_by="XM_077646273.1:135..2300"
                     /db_xref="GeneID:144113286"
ORIGIN      
        1 maypttyyhr maphpyplya nlpgfrract asssndcwlc dvltswnsal rycrlnlree
       61 epgelclqsl rgtffavyde prdkapvhsa afllaylprl hkcirrislh rehlphqpap
      121 vrlllgadww pqqpecnvqy leissivdve wdillcglgr vsalkgliie raildnnyvs
      181 kldqllaans gslqklvisa srtqtaeasd rliraisnct hltelsfdch ltttgvydlg
      241 rlllpceniq klslrdqfga errrallgal ssfvetnasl telhyksqsl dviqlldalk
      301 fnrtlkhlvl sgykhnqktf grehgaalga alatnsglrs lvishctfse lavedisagl
      361 alntslerfd lshcrvnfdv asalcrvlyt nrtlhmvvld pdgeaiserv rfskqvaaan
      421 cyhrvgmrwd etsmqnfvna lkqpslcppe chidssrfsi mslsqvcsal vlstvksisv
      481 tfidsnlnea rhhvheairq nqsivslslq edgissgaci fvakallenk tvtklslrin
      541 dlrtgaaetl seilsknqtl eklllncnhv dlwcvniiar glkenrtlth fsifderdda
      601 srsiitvyec vdrnlsswnc avqfvleaci srrraetfes lqgmpcflqr vaaasgksla
      661 eaaamvecak hfirsnylfi tgvvirgicc ypsgqtqvdc lindcwteit kylrvsdvir
      721 h
//
LOCUS       XP_077502400             721 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113287 [Amblyomma americanum].
ACCESSION   XP_077502400
VERSION     XP_077502400.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646274.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..721
                     /product="uncharacterized protein LOC144113287"
                     /calculated_mol_wt=80408
     Region          192..399
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     Region          193..221
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          222..248
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          249..279
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          280..304
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          338..365
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          <496..>589
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     CDS             1..721
                     /gene="LOC144113287"
                     /coded_by="XM_077646274.1:467..2632"
                     /db_xref="GeneID:144113287"
ORIGIN      
        1 maypttyyhr maphpyplya nlpgfrract asssndcwlc dvltswnsal qycrlnlree
       61 epgelclqsl rgaffavdde prdkapvhsa afllaylprl hkcirrislh rehlphqpap
      121 vrlllgadww pqqpecnvqy leissivdve wdillcglgr vsalkgliie raivdnnyvs
      181 kldqllaans gslqklvisa srtqtaeasd rliraisnct hltelsfdch ltrtgvydlg
      241 rlllpceniq klslrdqfga ecrrallgal rsfvetnasl telhykslsl dviqlldalk
      301 fnrtlkhlvl sgykhnqktf grehgaalga alatnsglrs lvishctfse lavedisagl
      361 alntslerfd lshcrvnfdv asalcrvlyt nrtlhvvvld pdgeaiserv rfskqvaaan
      421 cyhrvgmrwd etsmqnfvna lkqpslcppe chidssrfsi mslsqvcsal vlstvksisv
      481 tfidsnlnea rhhvheairq nqsivslslq edgissgaci fvakallenk tvtklslrin
      541 dlrtgaaetl seilsknqtl eklllncnhv dlwcvnviar glkenrtlth fsifderdda
      601 srstiavdec vdrnlsswnc avqfvleaci srrraetfes lqgmpcflqr vaaasgksla
      661 eaaamvecak hfirsnylfi tgvvirgicc ypsgqtqvdc lnndcwpeit kylrvsdvir
      721 h
//
LOCUS       XP_077502401             239 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial import inner membrane translocase subunit Tim21
            isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502401
VERSION     XP_077502401.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646275.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..239
                     /product="mitochondrial import inner membrane translocase
                     subunit Tim21 isoform X1"
                     /calculated_mol_wt=26611
     Region          86..227
                     /region_name="TIM21"
                     /note="pfam08294"
                     /db_xref="CDD:311963"
     CDS             1..239
                     /gene="LOC144113288"
                     /coded_by="XM_077646275.1:221..940"
                     /db_xref="GeneID:144113288"
ORIGIN      
        1 mtltskvhil vtgckakrlk nlsslahrsv ngqvqssdkd gallrltgfr pailwlrpas
       61 tttnkntsal seakdteggq ltlgqkvkqs tkdasylgvi laglgvtglm fytifhelfs
      121 ghspnsvysr alercradpr lkaalgeplq ghgettsrgr rrhvihmeyi kdginymrmk
      181 fyikgpsrsg tvhlevkeds hknyeyrylf vdldgypkrt iilednreik gpsntfdvl
//
LOCUS       XP_077502402             239 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial import inner membrane translocase subunit Tim21
            isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502402
VERSION     XP_077502402.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646276.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..239
                     /product="mitochondrial import inner membrane translocase
                     subunit Tim21 isoform X1"
                     /calculated_mol_wt=26611
     Region          86..227
                     /region_name="TIM21"
                     /note="pfam08294"
                     /db_xref="CDD:311963"
     CDS             1..239
                     /gene="LOC144113288"
                     /coded_by="XM_077646276.1:183..902"
                     /db_xref="GeneID:144113288"
ORIGIN      
        1 mtltskvhil vtgckakrlk nlsslahrsv ngqvqssdkd gallrltgfr pailwlrpas
       61 tttnkntsal seakdteggq ltlgqkvkqs tkdasylgvi laglgvtglm fytifhelfs
      121 ghspnsvysr alercradpr lkaalgeplq ghgettsrgr rrhvihmeyi kdginymrmk
      181 fyikgpsrsg tvhlevkeds hknyeyrylf vdldgypkrt iilednreik gpsntfdvl
//
LOCUS       XP_077502403             216 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial import inner membrane translocase subunit Tim21
            isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502403
VERSION     XP_077502403.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646277.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..216
                     /product="mitochondrial import inner membrane translocase
                     subunit Tim21 isoform X2"
                     /calculated_mol_wt=23668
     Region          86..199
                     /region_name="Coa1"
                     /note="Cytochrome oxidase complex assembly protein 1;
                     cl21648"
                     /db_xref="CDD:451344"
     CDS             1..216
                     /gene="LOC144113288"
                     /coded_by="XM_077646277.1:223..873"
                     /db_xref="GeneID:144113288"
ORIGIN      
        1 mtltskvhil vtgckakrlk nlsslahrsv ngqvqssdkd gallrltgfr pailwlrpas
       61 tttnkntsal seakdteggq ltlgqkvkqs tkdasylgvi laglgvtglm fytifhelfs
      121 ghspnsvysr alercradpr lkaalgeplq ghgettsrgr rrhvihmeyi kdginymrmk
      181 fyikgpsrsg tvhlevkevm fcsgqsqelr vplpvc
//
LOCUS       XP_077502404             399 aa            linear   INV 25-NOV-2025
DEFINITION  peptidase M20 domain-containing protein 2-like [Amblyomma
            americanum].
ACCESSION   XP_077502404
VERSION     XP_077502404.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646278.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..399
                     /product="peptidase M20 domain-containing protein 2-like"
                     /calculated_mol_wt=42777
     Region          12..372
                     /region_name="M20_ACY1L2-like"
                     /note="M20 Peptidase aminoacylase 1-like protein 2-like,
                     amidohydrolase subfamily; cd05672"
                     /db_xref="CDD:349921"
     CDS             1..399
                     /gene="LOC144113289"
                     /coded_by="XM_077646278.1:224..1423"
                     /db_xref="GeneID:144113289"
ORIGIN      
        1 msdmsefivs tidkyrekln lvsqtiwknp elyyrevkah dylsqalede gfqvqrkyhl
       61 dtafraeysn vlggpvicii ceydalpvig hacghnliae agfaagvavk ealrsdpklk
      121 gkvvvlgtpa eesgggkidl inagafadid vammvhpsrs nelaplfigv ghisveyhgk
      181 aahssgapwd gvnavdaavm cynglsylrq hikphcrvga iftnggaaan iipersamdi
      241 lyraptvamm eelrkkvmsc isagaeatgc smeitrsfsf kpivtnsvia qlykkhaeal
      301 gvefddanpk atlfvassdm gdvchtvpsi hpiyridtag pnhtkeftda aghpcaqept
      361 lvaakamala cldillnpdv lipkakaafa admeqscgr
//
LOCUS       XP_077502405            2006 aa            linear   INV 25-NOV-2025
DEFINITION  1-phosphatidylinositol 3-phosphate 5-kinase fab1 [Amblyomma
            americanum].
ACCESSION   XP_077502405
VERSION     XP_077502405.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646279.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2006
                     /product="1-phosphatidylinositol 3-phosphate 5-kinase
                     fab1"
                     /calculated_mol_wt=223960
     Region          156..217
                     /region_name="FYVE_PIKfyve_Fab1"
                     /note="FYVE domain found in metazoan PIKfyve, fungal and
                     plant Fab1, and similar proteins; cd15725"
                     /db_xref="CDD:277264"
     Site            order(157,160,178..183,185..186,210..212)
                     /site_type="other"
                     /note="putative phosphatidylinositol 3-phosphate binding
                     site [chemical binding]"
                     /db_xref="CDD:277264"
     Region          314..395
                     /region_name="DEP"
                     /note="DEP domain, named after Dishevelled, Egl-10, and
                     Pleckstrin, where this domain was first discovered. The
                     function of this domain is still not clear, but it is
                     believed to be important for the membrane association of
                     the signaling proteins in which it is...; cl02442"
                     /db_xref="CDD:470580"
     Region          574..831
                     /region_name="Fab1_TCP"
                     /note="TCP-1 like domain of the eukaryotic
                     phosphatidylinositol 3-phosphate (PtdIns3P) 5-kinase Fab1.
                     Fab1p is important for vacuole size regulation, presumably
                     by modulating PtdIns(3,5)P2 effector activity. In the
                     human homolog p235/PIKfyve deletion of this...; cd03334"
                     /db_xref="CDD:239450"
     Region          1725..1990
                     /region_name="PIPKc_PIKfyve"
                     /note="Phosphatidylinositol phosphate kinase (PIPK)
                     catalytic domain found in
                     1-phosphatidylinositol-3-phosphate 5-kinase and similar
                     proteins; cd17300"
                     /db_xref="CDD:340437"
     Site            order(1773,1782,1784,1844..1847,1858,1879,1926,1941..1942)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:340437"
     CDS             1..2006
                     /gene="fab1"
                     /coded_by="XM_077646279.1:428..6448"
                     /db_xref="GeneID:144113290"
ORIGIN      
        1 mdsadcrhga kkldsptslt efaplssdvk tkasgfsfsr ffklsrsprs datsdaavpe
       61 vvrdespcqc ipedeavapd pqslpcagte rdsptaasas taesqtvpss slpesraele
      121 vlhtrsfsgv lsriahimdr givapnaykd adfkqywmpd snckecyecg dkfttfrrrh
      181 hcricgqifc srccnreipg kiigyrgdlr vcmyccqvvl syvksldlsa dlrgvlsdlq
      241 nklgmmrmee edstspdtgt wgstgrrkts vgfreealvv pkgdtedysp sdkpgfqnps
      301 pgrtpvfdkm firelwsqiq hpihgapfqt yrhrlhsysn cvvgnelvdw lvaqgkvssr
      361 geaisigqal idsnflepvi phepifiday slykpssdtq tdahpshsss ppdevveenq
      421 dplwvseiqp agslspfavm ddslgvnkap pdcvdspmpy kekkrltast stfflnldlk
      481 esrvtvsrpr dslaispeee epedskrsrw dshrstinne vcnhtitddc vsgalyasss
      541 mnnlgqqkvl vdetktketk eslssaysvl ekdllnqlla adglplpwad vvlpivhkva
      601 stvtpnvkhl dddmdirqyv hikkipggnr secavvngvv ctknvvhkkm rqqltnpril
      661 lvgssivyqr venklssldp ilmqeceylk hvvakiqvfq pdlllvektv srlaqekllq
      721 mdvtlainvk psvmerisrc tqsaivssid aqlrkpnlgs cqnffvktya lpsnrfktll
      781 ffdgcsstlg ctillrggsa telkkvknii rfmlyvafnw rlelafltde haqppshsqv
      841 aeddieneve eknciayded pptsdqteep kvamgpedst rkvemssved fsdplhtlln
      901 adedeaalte hrlslksqvl plsnsfklal dstilccspf irltlpylkt eagrrcelrk
      961 ffsdeiywsp ktcdteevwt kaepdessls slsakntqek vvilphhpft fqklssslwe
     1021 saeaeallsd frarggriqn lcqhnfsktv hasgnspang aggtteqhmw dgrtdalspy
     1081 nhqrlavlfc sysptssnap yfcvnpwvvn mdfygrndip lggflerycf rpaytcpnas
     1141 cdtpmlehvr kfvhengciq illhhldnpa ataqssilmw swcrkcrfvt plttmsdetw
     1201 slsfakylel rfhghaytcr alheqcqhtl hhdyyqyfas qqivasfkys piamrevalp
     1261 ppvisivdei pqvsavvdei rdlalkgywv yntvvetlcs lraqvqatky eafaadlmem
     1321 qqseknafke kiediqlqlt spnlkmcqsq dpatldtsvl nevcslawki ndslvllkhy
     1381 iadavncwnk rlqdfesmrk reekllakts qmkkpasalg adayavsddq agvpkqelne
     1441 sidtasslgd sdsagdadia dghfsldlhh cdkevqdiqr ddnvpflpdd sfspptgptm
     1501 lhvssssssv sscmygispg mqvghlgqds gtpkqdsscg qapglddste skksttlrsh
     1561 ersrsdgaer llenrtkltt askveddtpg gkqekrgtvk tiisqllsss gsnpiqspfp
     1621 lsdhfnvshg aripiviydq epssiiahal asadyeqklv elqstlttal nqlreqpspt
     1681 akaptdnsmv dfndlalcgs qetdkrtghk npnmhietqf sdatsqfycr iffaeqfrkl
     1741 rclifphged rfihslsrcv swsaqggksg ssfckthddr filkqmsrye vqsflefapl
     1801 yfqyvssact drqptvlaki vgvfrigykn satnaaskld llvmenlfyk rniaqkfdlk
     1861 gsvrnrmvnt rsqtegdvvl ldenllkttc dsplyvrphs ktvlslairn danflsdnsv
     1921 mdysllvgfd ndrkelvvgi idyirtftwd kklemvvkst vtlrghgklp tivwpnlyrv
     1981 rfceamdkyf lcvpdhwsgl grgvec
//
LOCUS       XP_077502406             350 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger TRAF-type-containing protein 1 homolog isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077502406
VERSION     XP_077502406.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646280.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..350
                     /product="zinc finger TRAF-type-containing protein 1
                     homolog isoform X1"
                     /calculated_mol_wt=39598
     Region          46..107
                     /region_name="RING-HC_CYHR1"
                     /note="RING finger, HC subclass, found in cysteine and
                     histidine-rich protein 1 (CYHR1) and similar proteins;
                     cd16505"
                     /db_xref="CDD:438168"
     Region          112..>183
                     /region_name="Sina"
                     /note="Seven in absentia protein family; pfam03145"
                     /db_xref="CDD:460824"
     Region          195..350
                     /region_name="CYHR1_C"
                     /note="C-terminal domain of cysteine and histidine-rich
                     protein 1 and similar proteins; cd22861"
                     /db_xref="CDD:439370"
     CDS             1..350
                     /gene="LOC144113291"
                     /coded_by="XM_077646280.1:141..1193"
                     /db_xref="GeneID:144113291"
ORIGIN      
        1 madetgssvs gselaestsd eclgeaegpa kkrfkagerl lgksdklehr lggilccavc
       61 ldlprsaiyq ctnghlmcag cfthlladar lrdetatcpn crtvisrelc srnlavekav
      121 celptecvfc gselpraqie rheaelceer ltrcqysrig cqwrgpyhel evhsqvcshp
      181 hksggevmea lelidqqmlk eqmlyntife llsvekitfn dlqfkpyrtd efihklyyet
      241 srftafssqw vvkarvndnq rdptqscert lsyhlvlksr altpmgihym vlkgpfgdlk
      301 vnprlyqhef teaamespyq plplpdsaec nkllaakafn frlimfhvdk
//
LOCUS       XP_077502407             300 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger TRAF-type-containing protein 1 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502407
VERSION     XP_077502407.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646281.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..300
                     /product="zinc finger TRAF-type-containing protein 1
                     isoform X2"
                     /calculated_mol_wt=33379
     Region          46..107
                     /region_name="RING-HC_CYHR1"
                     /note="RING finger, HC subclass, found in cysteine and
                     histidine-rich protein 1 (CYHR1) and similar proteins;
                     cd16505"
                     /db_xref="CDD:438168"
     Region          112..>183
                     /region_name="Sina"
                     /note="Seven in absentia protein family; pfam03145"
                     /db_xref="CDD:460824"
     Region          195..>258
                     /region_name="CYHR1_C"
                     /note="C-terminal domain of cysteine and histidine-rich
                     protein 1 and similar proteins; cd22861"
                     /db_xref="CDD:439370"
     CDS             1..300
                     /gene="LOC144113291"
                     /coded_by="XM_077646281.1:141..1043"
                     /db_xref="GeneID:144113291"
ORIGIN      
        1 madetgssvs gselaestsd eclgeaegpa kkrfkagerl lgksdklehr lggilccavc
       61 ldlprsaiyq ctnghlmcag cfthlladar lrdetatcpn crtvisrelc srnlavekav
      121 celptecvfc gselpraqie rheaelceer ltrcqysrig cqwrgpyhel evhsqvcshp
      181 hksggevmea lelidqqmlk eqmlyntife llsvekitfn dlwesttwcs rapsetsrsi
      241 hactsmnsqk lpwrarinrc hcpillnats cllpkpstsa sscftwtset mcrclesdml
//
LOCUS       XP_077502408             323 aa            linear   INV 25-NOV-2025
DEFINITION  neuralized-like protein 2 [Amblyomma americanum].
ACCESSION   XP_077502408
VERSION     XP_077502408.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646282.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..323
                     /product="neuralized-like protein 2"
                     /calculated_mol_wt=35226
     Region          41..276
                     /region_name="SPRY_NHR_like"
                     /note="SPRY domain in neuralized homology repeat; cd12887"
                     /db_xref="CDD:293945"
     Region          282..317
                     /region_name="SOCS_SOCS_like"
                     /note="SOCS (suppressors of cytokine signaling) box of
                     SOCS-like proteins. The CIS/SOCS family of proteins is
                     characterized by the presence of a C-terminal SOCS box and
                     a central SH2 domain. These intracellular proteins
                     regulate the responses of immune cells to...; cd03717"
                     /db_xref="CDD:239687"
     Site            order(282..286,292,299,305)
                     /site_type="active"
                     /note="elongin B/C interaction [active]"
                     /db_xref="CDD:239687"
     CDS             1..323
                     /gene="LOC144113292"
                     /coded_by="XM_077646282.1:247..1218"
                     /db_xref="GeneID:144113292"
ORIGIN      
        1 mhvclssndg sfggtmnvtt rnevvhkwkd asgstsvpss rfhsyhgrnl ilcdnnmvay
       61 rkksfahala fseqpllpge iflleiaqne rgwsghmrlg ltqhdpgchf plpqyalpdl
      121 anmgrswvfa ihnsetdseh pgsspyqrld tstsvlgtge hiytsrgvvp rsmllpkkrp
      181 rtaimgtpvg gedsvagfsg dgsfnsgtgs cgkssilptd vgsrigvmyv trgqsadmhf
      241 iingedqlya tdiphqdkpl yavvdvygtt khvrivqlyg vvaslqsacr dailqhissc
      301 avrtlplprk lkeylcyhsp rpq
//
LOCUS       XP_077502409             323 aa            linear   INV 25-NOV-2025
DEFINITION  neuralized-like protein 2 [Amblyomma americanum].
ACCESSION   XP_077502409
VERSION     XP_077502409.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646283.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..323
                     /product="neuralized-like protein 2"
                     /calculated_mol_wt=35226
     Region          41..276
                     /region_name="SPRY_NHR_like"
                     /note="SPRY domain in neuralized homology repeat; cd12887"
                     /db_xref="CDD:293945"
     Region          282..317
                     /region_name="SOCS_SOCS_like"
                     /note="SOCS (suppressors of cytokine signaling) box of
                     SOCS-like proteins. The CIS/SOCS family of proteins is
                     characterized by the presence of a C-terminal SOCS box and
                     a central SH2 domain. These intracellular proteins
                     regulate the responses of immune cells to...; cd03717"
                     /db_xref="CDD:239687"
     Site            order(282..286,292,299,305)
                     /site_type="active"
                     /note="elongin B/C interaction [active]"
                     /db_xref="CDD:239687"
     CDS             1..323
                     /gene="LOC144113292"
                     /coded_by="XM_077646283.1:248..1219"
                     /db_xref="GeneID:144113292"
ORIGIN      
        1 mhvclssndg sfggtmnvtt rnevvhkwkd asgstsvpss rfhsyhgrnl ilcdnnmvay
       61 rkksfahala fseqpllpge iflleiaqne rgwsghmrlg ltqhdpgchf plpqyalpdl
      121 anmgrswvfa ihnsetdseh pgsspyqrld tstsvlgtge hiytsrgvvp rsmllpkkrp
      181 rtaimgtpvg gedsvagfsg dgsfnsgtgs cgkssilptd vgsrigvmyv trgqsadmhf
      241 iingedqlya tdiphqdkpl yavvdvygtt khvrivqlyg vvaslqsacr dailqhissc
      301 avrtlplprk lkeylcyhsp rpq
//
LOCUS       XP_077502410             129 aa            linear   INV 25-NOV-2025
DEFINITION  BEN domain-containing protein 6-like [Amblyomma americanum].
ACCESSION   XP_077502410
VERSION     XP_077502410.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646284.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..129
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..129
                     /product="BEN domain-containing protein 6-like"
                     /calculated_mol_wt=13944
     Region          57..>114
                     /region_name="BEN"
                     /note="The BEN domain is found in diverse animal proteins;
                     smart01025"
                     /db_xref="CDD:214981"
     CDS             1..129
                     /gene="LOC144113295"
                     /coded_by="XM_077646284.1:431..820"
                     /db_xref="GeneID:144113295"
ORIGIN      
        1 mldaakmves lkkmvdtmek mpartaesps evnigngvti pkvlldkwka mcptpprfaw
       61 allrsvftse elqgcslfgq rsnlwpgapv kpaldknkvd aiigytcslf nttdtkvkas
      121 latmlakgk
//
LOCUS       XP_077502412            2401 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: CCR4-NOT transcription complex subunit 1
            [Amblyomma americanum].
ACCESSION   XP_077502412
VERSION     XP_077502412.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646286.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 3 indels
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2401
                     /product="LOW QUALITY PROTEIN: CCR4-NOT transcription
                     complex subunit 1"
                     /calculated_mol_wt=267816
     Region          496..652
                     /region_name="CNOT1_HEAT"
                     /note="CCR4-NOT transcription complex subunit 1 HEAT
                     repeat; pfam16418"
                     /db_xref="CDD:465113"
     Region          <808..2392
                     /region_name="CDC39"
                     /note="Cell division control protein, negative regulator
                     of transcription [Cell division and chromosome
                     partitioning / Transcription]; COG5103"
                     /db_xref="CDD:227434"
     CDS             1..2401
                     /gene="Not1"
                     /coded_by="XM_077646286.1:316..7521"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 4 bases in 2 codons;
                     deleted 1 base in 1 codon"
                     /db_xref="GeneID:144113297"
ORIGIN      
        1 mnldplsfal syisysvssl tkknfkssvq eisrlvalhg feaerhllrc lfshvdfsgd
       61 gkssgkdfhq tqyliqefss ilakpnfvss vcfaienplh hqkslrpspl llphisrvlr
      121 lnrvqevvlg tsllhsssae lchcatqfir lklpdllrsy tdsdsstqeg dlqdctpevl
      181 hlllvellnk nsehfgvtne lkeaffenlr kdfpqdrvpv vlapllygea sdvlmakgmt
      241 epssnmskpm elshvdvike vgygftssve ecrqslvqmg lrdpgpcmvs rvlsmmanth
      301 sglseplqgl gsstttawga dkdanasnvt wnidvfiqal ydlvptlnwk dvvseldfpg
      361 ffvkdrqglk llwhglcrgl gsepfptdrl yrvwrnpdsq vslfhqimkn sdivcltdyk
      421 yrpvsmeilk alpeddnrdv snwksldlie tllnlsekvq ysshvhdlfk ypvqhcpdll
      481 mlgllqtsgp mqgfrqellt nlvfvflsnh pnsaivlqaa wnvqshanvv rplimhtmad
      541 wymrggeseq grlsrildva qdlkalsvll ssnsfplvid laclasrrey lkldkwlndk
      601 ifehkepfiq acvsflkrrc pqimggivke enlpksaqlp tetlatmlsc lqqyaggvsq
      661 elsetiltma gncsillnkp rqpppgvvkn atrawsqhfq qpvsthlrll sssnsssnns
      721 snssssrqql evlsslgsls isgpaaaqpp plgvgggppl fgssfatpgs pasxfgapps
      781 gtpsngpppp aqtasfssvv pslgtxqfgs qldvatilpe mnqpvskeie deangyfqri
      841 ynhpphptls iaevldmlkr fqespikser dvfncmiknl feeyrffpqy pekeliitaq
      901 lfggiieqsl vtymalglal ryvldalrkp ygtkmyyfgi taldrfktrl kdypqycqhl
      961 asiphfkdfp ehlreyvdcg arsqeplnrp qgqtlphnla mlaqglnpaa tpqnsvvrtt
     1021 patvptssag laatsgvvak ptnvggkpsi anatnidtll gatekddklv ippeavqdkv
     1081 afiinnlsqm nlpqkteefk evvkeefwpw faqymvmkra siepnfhtly anfldtlkig
     1141 elsklvlret frnikvllts dkalanfsdr sllkslghwl gmltmaknkp vlqvdmdvks
     1201 llveayhkgq qellyvvpfi akvlescaks rvfkppnpwt mgildvlsel hrepelklnl
     1261 kfeievlckt lnldindlkp glilkdqerr krldpqlspr hkvespplpp svvaptvaps
     1321 avppplasqg pagvvtavag pgvpvaqvgl apqqpeevps amsvtgtsma afpqpqfsyh
     1381 dinvlsmtnl aqhiqinsql plfqahphlk qyvrtaidra iqewimpvve rslkialttc
     1441 dqivrkdfal dpeesrlraa ahhmvrslta gmamitcrdp mlmtisnnlk aafhtalrlq
     1501 gatlpqkell eqattvvaqe nvelaccfiq ktaiekaipe idkklgqeye frksarsegr
     1561 rycdpvvlty qaermpeqir lkvgcvtpqq mavyeefarn ipgfqpiser dqpslflpkp
     1621 vvrqtgfass vddmthlydk ivseleqqvh gllgtvvpsn pqvaalhvlh eavlvarnsr
     1681 eavtamallq ktvesmaegl shslgadpet avrfrdahla vlktlaegyg hpwaakhitr
     1741 fllelreeir wnvdvvdpll rsgllvlatf dlhlaqsmen gmnfpavdfa kqlvrlyfse
     1801 arhgdynlse sdlcntletl vrinahsrqp qeglsqvmem lrsgsgggav eyggvpssgp
     1861 msmmhsgisq areyddppgl hektefllre wvsmynapta gkdstkafqi fvqqmnvqgi
     1921 lktddlitrf frlstemcve hcyralmeqt psnativrsk cfnsldaymr liallvklsg
     1981 dasnpttkvn llnkvlgiva gvllqdhesr glefqqlpyh rifmmlflel sapepvleai
     2041 gfhvlmafcn tlhvlqpcka pafayswlel ishrvflgrv laltpqqkaw gmfaqllndl
     2101 fkflapflrn vdlekpiqll ykgtlrvllv llhdfpeflc dyhygfcdli panciqmrnl
     2161 ilsafprhmr lpdpftpnlk vevlpeitqa prvltnfasv iqpqsfkkdl dsyiktrgpv
     2221 tflselrsnl qattepginy nvplmnalvl yvgtqaiayi qsksltpsms tithsshmdi
     2281 fqnlavdldt egrylflnai anqlrypnsh thyfsctlly lfaeanteai qeqitrvlle
     2341 rlivnrphpw gllvtfidli knptfkfwth efvrcapeie klfesvarsc mqqkppteps
     2401 g
//
LOCUS       XP_077502413             428 aa            linear   INV 25-NOV-2025
DEFINITION  DENN domain-containing protein 11-like [Amblyomma americanum].
ACCESSION   XP_077502413
VERSION     XP_077502413.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646287.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..428
                     /product="DENN domain-containing protein 11-like"
                     /calculated_mol_wt=48678
     Region          63..313
                     /region_name="DENND11"
                     /note="DENN domain-containing protein 11; pfam09804"
                     /db_xref="CDD:430838"
     CDS             1..428
                     /gene="LOC144113299"
                     /coded_by="XM_077646287.1:228..1514"
                     /db_xref="GeneID:144113299"
ORIGIN      
        1 marrkgatdr apllsddepi dgystytnis cqmkcqlgpa lprtdsiktn klgddtvydi
       61 vgvfviafdt ragntvewwl pddldvsgie fkamtsgahr vhtdfiyfkr knyyglscfe
      121 nmkveseqer garmksigvl aksysllhlh kaflqkqvrq llerpgvyed lfqfylahks
      181 ppaldtflgt ayksicdgmh smeithpagc fsqflnffge kifllwkfal lkkrilffsp
      241 ppigvicyrv yctstlvahv ypdvetclcp pnfyvnvtdv dalaeqlsyv acttekifet
      301 kqnlfdlfvd qqnlkvhssl herllelspa drlkynhlle lrskcqplla gdsdatddsw
      361 ftgffmaqnt qlfkellvvs rsteklwtde hmhrvgldph gdklfltelv erygidivli
      421 tdsvccpa
//
LOCUS       XP_077502414             694 aa            linear   INV 25-NOV-2025
DEFINITION  putative ATP-dependent RNA helicase DHX35 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502414
VERSION     XP_077502414.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646288.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..694
                     /product="putative ATP-dependent RNA helicase DHX35
                     isoform X1"
                     /calculated_mol_wt=77758
     Region          50..>693
                     /region_name="HrpA"
                     /note="HrpA-like RNA helicase [Translation, ribosomal
                     structure and biogenesis]; COG1643"
                     /db_xref="CDD:441249"
     CDS             1..694
                     /gene="LOC144113300"
                     /coded_by="XM_077646288.1:230..2314"
                     /db_xref="GeneID:144113300"
ORIGIN      
        1 mfqrpvfqkp ggereflnee wiieevtgsa eytnyvynpn vslsisqqre rlpvfknrid
       61 ilyllekhrv livtgetgsg kstqipqylm eagwaqkgqm igvtqprrva aislarrvae
      121 ekgclvgqev gycvrfddcf dkertkikfm tegilvneim aspllpsysv lmldeahert
      181 lltdtslglm kkillkrpdl rliissatle aevlksffhs dieskhisse ilsiqgrvyp
      241 vevyyltnpv pnyvkaavet vvkihetqrm ghvlvfltgq deveeavsll ieysrntknt
      301 pgipsmyvlp lygslpqseq mkafqpfspk vrkvvvatni aeasvtingi vyvvdcgfvk
      361 lnffnpktst dalvvvpesq ssatqragra grvssgkvyr lfreedfkql plfttpeiqr
      421 snlstlvlql kslgvnniah fsfpsappsk ivinaleldy algaldksgg ltelgmkmvt
      481 fpvpamqakm lltsgefgcs eealtivaml qiesiflfps srraearkak ykfsvlegdl
      541 ltllnvyngf iqkgkdkqwc gqmflnyngl vrateirnql rklllkykvp lvscqgdtds
      601 vcrcivaghf ayaaylhysg eyrtvcgdhp laihptsvly tqkkpkwvvf gelvhtsref
      661 mrnvtvvess wlyelapgfy qygtdtdaar grll
//
LOCUS       XP_077502415             603 aa            linear   INV 25-NOV-2025
DEFINITION  putative ATP-dependent RNA helicase DHX35 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502415
VERSION     XP_077502415.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646289.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..603
                     /product="putative ATP-dependent RNA helicase DHX35
                     isoform X2"
                     /calculated_mol_wt=67901
     Region          50..>602
                     /region_name="HrpA"
                     /note="HrpA-like RNA helicase [Translation, ribosomal
                     structure and biogenesis]; COG1643"
                     /db_xref="CDD:441249"
     CDS             1..603
                     /gene="LOC144113300"
                     /coded_by="XM_077646289.1:229..2040"
                     /db_xref="GeneID:144113300"
ORIGIN      
        1 mfqrpvfqkp ggereflnee wiieevtgsa eytnyvynpn vslsisqqre rlpvfknrid
       61 ilyllekhrv livtgetgsg kstqipqylm eagwaqkgqm igvtqprrva aislarrvae
      121 ekgclvgqev gycvrfddcf dkertkikfm tegilvneim aspllpsysv lmldeahert
      181 lltdtslglm kkillkrpdl rliissatle aevlksffhs dieskhisse ilsiqgrvyp
      241 vevyyltnpv pnyvkaavet vvkihetqrm ghvlvfltgq deveeavsll ieysrntknt
      301 pgipsmyvlp lygslpqseq mkafqpfspk vrkvvvatni aeasvtingi vyvvdcgfvk
      361 lnffnpktst dalvvvpesq ssatqragra grvssgkvyr lfrgefgcse ealtivamlq
      421 iesiflfpss rraearkaky kfsvlegdll tllnvyngfi qkgkdkqwcg qmflnynglv
      481 rateirnqlr klllkykvpl vscqgdtdsv crcivaghfa yaaylhysge yrtvcgdhpl
      541 aihptsvlyt qkkpkwvvfg elvhtsrefm rnvtvvessw lyelapgfyq ygtdtdaarg
      601 rll
//
LOCUS       XP_077502416             642 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113303 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502416
VERSION     XP_077502416.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646290.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..642
                     /product="uncharacterized protein LOC144113303 isoform X1"
                     /calculated_mol_wt=71979
     Region          428..>505
                     /region_name="zf-CCCH_3"
                     /note="Zinc-finger containing family; pfam15663"
                     /db_xref="CDD:464787"
     CDS             1..642
                     /gene="LOC144113303"
                     /coded_by="XM_077646290.1:259..2187"
                     /db_xref="GeneID:144113303"
ORIGIN      
        1 maqtdhekka lqdeisylsr lidshhfltk tqestqigsl srykkvnapv trarsavcsg
       61 grftnvlkvd phcstrsten vgsqdssivr ntfglqrtpq prsrstvvhk srytltklha
      121 rpnttekpel kpgilearkl lsvppatvtc talschtsfk rppsgryvyr rksetngtac
      181 sngrfgtaps npsmksprpl vstyrlirnr akaatpiksp ssnltrlgar rivkkyklnn
      241 vnksrtslqr pqrlqferca amsgmkrfwp psyssthyfh gqkrkmtgks stdlntthsh
      301 yikvgnityk asrnklsref rrslsqspva ckaspcheri itvrgsvytm dktgkvlrri
      361 sqprrnsfas gltridvggq tyieqkpgvl sqtpsaetrt ylsravnrsi qrvrtvntsr
      421 rlqwksycif fnrfgrcnkg dscgyihdpe kvavctrylr gtckvsdcqf shkvapekmp
      481 vcsfflkgrc tnnpcpyrhv kvsskaevcq dfavrgycad gikckkqhil icpehaankk
      541 cprgnkcyls hrargtkrkc eedprqgnle hssragaplk apkqswscpg psrsmeseqe
      601 aqettkapqf iplesssplp vtpvarscpg etgirirpnf lt
//
LOCUS       XP_077502417             642 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113303 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502417
VERSION     XP_077502417.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646291.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..642
                     /product="uncharacterized protein LOC144113303 isoform X1"
                     /calculated_mol_wt=71979
     Region          428..>505
                     /region_name="zf-CCCH_3"
                     /note="Zinc-finger containing family; pfam15663"
                     /db_xref="CDD:464787"
     CDS             1..642
                     /gene="LOC144113303"
                     /coded_by="XM_077646291.1:265..2193"
                     /db_xref="GeneID:144113303"
ORIGIN      
        1 maqtdhekka lqdeisylsr lidshhfltk tqestqigsl srykkvnapv trarsavcsg
       61 grftnvlkvd phcstrsten vgsqdssivr ntfglqrtpq prsrstvvhk srytltklha
      121 rpnttekpel kpgilearkl lsvppatvtc talschtsfk rppsgryvyr rksetngtac
      181 sngrfgtaps npsmksprpl vstyrlirnr akaatpiksp ssnltrlgar rivkkyklnn
      241 vnksrtslqr pqrlqferca amsgmkrfwp psyssthyfh gqkrkmtgks stdlntthsh
      301 yikvgnityk asrnklsref rrslsqspva ckaspcheri itvrgsvytm dktgkvlrri
      361 sqprrnsfas gltridvggq tyieqkpgvl sqtpsaetrt ylsravnrsi qrvrtvntsr
      421 rlqwksycif fnrfgrcnkg dscgyihdpe kvavctrylr gtckvsdcqf shkvapekmp
      481 vcsfflkgrc tnnpcpyrhv kvsskaevcq dfavrgycad gikckkqhil icpehaankk
      541 cprgnkcyls hrargtkrkc eedprqgnle hssragaplk apkqswscpg psrsmeseqe
      601 aqettkapqf iplesssplp vtpvarscpg etgirirpnf lt
//
LOCUS       XP_077502418             642 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113303 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502418
VERSION     XP_077502418.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646292.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..642
                     /product="uncharacterized protein LOC144113303 isoform X1"
                     /calculated_mol_wt=71979
     Region          428..>505
                     /region_name="zf-CCCH_3"
                     /note="Zinc-finger containing family; pfam15663"
                     /db_xref="CDD:464787"
     CDS             1..642
                     /gene="LOC144113303"
                     /coded_by="XM_077646292.1:268..2196"
                     /db_xref="GeneID:144113303"
ORIGIN      
        1 maqtdhekka lqdeisylsr lidshhfltk tqestqigsl srykkvnapv trarsavcsg
       61 grftnvlkvd phcstrsten vgsqdssivr ntfglqrtpq prsrstvvhk srytltklha
      121 rpnttekpel kpgilearkl lsvppatvtc talschtsfk rppsgryvyr rksetngtac
      181 sngrfgtaps npsmksprpl vstyrlirnr akaatpiksp ssnltrlgar rivkkyklnn
      241 vnksrtslqr pqrlqferca amsgmkrfwp psyssthyfh gqkrkmtgks stdlntthsh
      301 yikvgnityk asrnklsref rrslsqspva ckaspcheri itvrgsvytm dktgkvlrri
      361 sqprrnsfas gltridvggq tyieqkpgvl sqtpsaetrt ylsravnrsi qrvrtvntsr
      421 rlqwksycif fnrfgrcnkg dscgyihdpe kvavctrylr gtckvsdcqf shkvapekmp
      481 vcsfflkgrc tnnpcpyrhv kvsskaevcq dfavrgycad gikckkqhil icpehaankk
      541 cprgnkcyls hrargtkrkc eedprqgnle hssragaplk apkqswscpg psrsmeseqe
      601 aqettkapqf iplesssplp vtpvarscpg etgirirpnf lt
//
LOCUS       XP_077502419             381 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113303 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502419
VERSION     XP_077502419.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646293.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..381
                     /product="uncharacterized protein LOC144113303 isoform X2"
                     /calculated_mol_wt=42836
     Region          167..>244
                     /region_name="zf-CCCH_3"
                     /note="Zinc-finger containing family; pfam15663"
                     /db_xref="CDD:464787"
     Region          <220..>333
                     /region_name="YTH1"
                     /note="Cleavage and polyadenylation specificity factor
                     (CPSF) Clipper subunit and related makorin family
                     Zn-finger proteins [General function prediction only];
                     COG5084"
                     /db_xref="CDD:227416"
     CDS             1..381
                     /gene="LOC144113303"
                     /coded_by="XM_077646293.1:501..1646"
                     /db_xref="GeneID:144113303"
ORIGIN      
        1 msgmkrfwpp syssthyfhg qkrkmtgkss tdlntthshy ikvgnityka srnklsrefr
       61 rslsqspvac kaspcherii tvrgsvytmd ktgkvlrris qprrnsfasg ltridvggqt
      121 yieqkpgvls qtpsaetrty lsravnrsiq rvrtvntsrr lqwksyciff nrfgrcnkgd
      181 scgyihdpek vavctrylrg tckvsdcqfs hkvapekmpv csfflkgrct nnpcpyrhvk
      241 vsskaevcqd favrgycadg ikckkqhili cpehaankkc prgnkcylsh rargtkrkce
      301 edprqgnleh ssragaplka pkqswscpgp srsmeseqea qettkapqfi plesssplpv
      361 tpvarscpge tgirirpnfl t
//
LOCUS       XP_077502420             900 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113305 [Amblyomma americanum].
ACCESSION   XP_077502420
VERSION     XP_077502420.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646294.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..900
                     /product="uncharacterized protein LOC144113305"
                     /calculated_mol_wt=99894
     CDS             1..900
                     /gene="LOC144113305"
                     /coded_by="XM_077646294.1:1250..3952"
                     /db_xref="GeneID:144113305"
ORIGIN      
        1 msqdsqpatp krrklylene srpfvvprst awfnkkrslt spatsvngnp tyvsdsasve
       61 sgasssaagp sqpsseshfg aaaetgssdk ccqlddnsga dnsslshtde ieprmdatse
      121 gdcsssssss eygdpddged dgaavphgst vpqfdsaell secmqefgrk tlpgssttla
      181 eavvlimafv vangltwsal ddllklvdal fgyqrsglph skylfrklwa gkcaslvqrh
      241 fycevcmsll qacsdsqmls ckvcktqvew rtvkkrgcff allnlkeqlh lvtdkvgsll
      301 fanmsklaea assgskiitd ignaavckgm rqqgllkwsd ftltvntdgs plfksnnssv
      361 wpiqvcvnel pqphrmensf laglwfgrkh pdmmlfvgef vkavqavgev vwqhgaaava
      421 skvyiacvcv daparasvgn htqfnglfgc pwclacgkle egrrlytste ppaeertskg
      481 vrknmklaml qktsvnglkg psplwslkyl dlvscytvey mhcvllgvtr qfteywfdps
      541 rcrekyyigr pstvnalnkr ltsirpphhv trlprtiqer hfwkahewrn wllfycvpcc
      601 lntlpaqflr hfallceaif illqeqlssa aisyadrllq rfvcraaaly gercmsfnvh
      661 qlthltqaar nfgplwahsa fgfesgnshi vklvtaangv pdqileraim sqelhlvlsl
      721 naiplrsqql cseflhypnv skahyvqgac mfgvpkevpl llpaeraale lmlnniprvq
      781 eyfrffyrgt vmhsqqyqrg tksdssvies qdgeffvikr ifevvdegsp sngevvllcr
      841 kivcedcdly lpghimqcff slefplvaln lsgvakpcif vtfsneenyv ckipnlierd
//
LOCUS       XP_077502421             431 aa            linear   INV 25-NOV-2025
DEFINITION  OXA1L mitochondrial inner membrane protein isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502421
VERSION     XP_077502421.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646295.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..431
                     /product="OXA1L mitochondrial inner membrane protein
                     isoform X1"
                     /calculated_mol_wt=47504
     Region          145..343
                     /region_name="5TM_Oxa1-like"
                     /note="Five transmembrane core domain of mitochondrial
                     inner membrane protein Oxa1 and similar proteins; cd20069"
                     /db_xref="CDD:410993"
     CDS             1..431
                     /gene="OXA1L"
                     /coded_by="XM_077646295.1:92..1387"
                     /db_xref="GeneID:144113306"
ORIGIN      
        1 mntnctlkyg iqitlnkrtg lwnslrrqpa rllsakccqr vpllkpnnrk yappicqvsg
       61 srwlsseagk idtvaatvgq veqttsaaaa aipavppapt vvpvesfvqs laddtvptlq
      121 svglggwsps givqqlldfl hastglpwwa tiaistvvvk vcllplvikg qrnsihmnnn
      181 lpqmqhlqak mtearntgnq leaarianel mvfmkeknin plksmiipma qapvfisfff
      241 alrgmanlpm esfktggmlw ftdltvadpy yllplitsvs lfctlelgae sgvradnlqw
      301 tryvfrclpv vifpitmnfp sallcywvts nmftlcqvgv lrieavrkrl nmpvllkhqk
      361 selalskkgf vegvresftn triareleer srtdeirfkk agigpvvkty tydptkqafa
      421 rgagssqkvn g
//
LOCUS       XP_077502422             430 aa            linear   INV 25-NOV-2025
DEFINITION  OXA1L mitochondrial inner membrane protein isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502422
VERSION     XP_077502422.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646296.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..430
                     /product="OXA1L mitochondrial inner membrane protein
                     isoform X2"
                     /calculated_mol_wt=47376
     Region          144..342
                     /region_name="5TM_Oxa1-like"
                     /note="Five transmembrane core domain of mitochondrial
                     inner membrane protein Oxa1 and similar proteins; cd20069"
                     /db_xref="CDD:410993"
     CDS             1..430
                     /gene="OXA1L"
                     /coded_by="XM_077646296.1:92..1384"
                     /db_xref="GeneID:144113306"
ORIGIN      
        1 mntnctlkyg iqitlnkrtg lwnslrrqpa rllsakccqr vpllkpnnrk yappicqvsg
       61 srwlsseagk idtvaatvgv eqttsaaaaa ipavppaptv vpvesfvqsl addtvptlqs
      121 vglggwspsg ivqqlldflh astglpwwat iaistvvvkv cllplvikgq rnsihmnnnl
      181 pqmqhlqakm tearntgnql eaarianelm vfmkekninp lksmiipmaq apvfisfffa
      241 lrgmanlpme sfktggmlwf tdltvadpyy llplitsvsl fctlelgaes gvradnlqwt
      301 ryvfrclpvv ifpitmnfps allcywvtsn mftlcqvgvl rieavrkrln mpvllkhqks
      361 elalskkgfv egvresftnt riareleers rtdeirfkka gigpvvktyt ydptkqafar
      421 gagssqkvng
//
LOCUS       XP_077502423             255 aa            linear   INV 25-NOV-2025
DEFINITION  OXA1L mitochondrial inner membrane protein isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502423
VERSION     XP_077502423.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646297.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..255
                     /product="OXA1L mitochondrial inner membrane protein
                     isoform X3"
                     /calculated_mol_wt=28717
     Region          1..167
                     /region_name="5TM_Oxa1-like"
                     /note="Five transmembrane core domain of mitochondrial
                     inner membrane protein Oxa1 and similar proteins; cd20069"
                     /db_xref="CDD:410993"
     CDS             1..255
                     /gene="OXA1L"
                     /coded_by="XM_077646297.1:570..1337"
                     /db_xref="GeneID:144113306"
ORIGIN      
        1 mnnnlpqmqh lqakmtearn tgnqleaari anelmvfmke kninplksmi ipmaqapvfi
       61 sfffalrgma nlpmesfktg gmlwftdltv adpyyllpli tsvslfctle lgaesgvrad
      121 nlqwtryvfr clpvvifpit mnfpsallcy wvtsnmftlc qvgvlrieav rkrlnmpvll
      181 khqkselals kkgfvegvre sftntriare leersrtdei rfkkagigpv vktytydptk
      241 qafargagss qkvng
//
LOCUS       XP_077502424             307 aa            linear   INV 25-NOV-2025
DEFINITION  female sterile (2) ltoPP43 [Amblyomma americanum].
ACCESSION   XP_077502424
VERSION     XP_077502424.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646298.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..307
                     /product="female sterile (2) ltoPP43"
                     /calculated_mol_wt=34203
     Region          203..270
                     /region_name="Iwr1"
                     /note="Transcription factor Iwr1; pfam08574"
                     /db_xref="CDD:430085"
     CDS             1..307
                     /gene="fs(2)ltoPP43"
                     /coded_by="XM_077646298.1:304..1227"
                     /db_xref="GeneID:144113307"
ORIGIN      
        1 mttavrikrk ageiaadaiv lapkisrlgd gdqaigggea dytfyyagsf rnpnsseaeq
       61 virhakeakr lkqdpklrpv dlqnklrney rsatkkkrqq tilrhraive elcteeaidt
      121 dvnalkesan slfrlydvht edaeadqask envdskeqek tskiacnnva mvqetvkepk
      181 itcnnvavvq etvkgpkeda eciydvyyas eefkllrddg ynlfkiggpp dafapetayd
      241 sdsdlglevh dddsdsnaed nwrndypded fekwasysgg lfdegtdfms gqlndltldf
      301 ddpdadg
//
LOCUS       XP_077502425             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113309 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502425
VERSION     XP_077502425.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646299.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144113309 isoform X1"
                     /calculated_mol_wt=19307
     CDS             1..181
                     /gene="LOC144113309"
                     /coded_by="XM_077646299.1:186..731"
                     /db_xref="GeneID:144113309"
ORIGIN      
        1 mrwmrnrdav gldpasmdap plppltagtw ssagipwqhe adarghlaga tqpalllllt
       61 raaeaapras wshqtlatvm eqnntlsvim fmamfggstf llfllcyccq rkmrenafap
      121 ldnastahhp qvaanlasqs tqiipsgppp nydavvkkdp adlepppyev avaslqasgy
      181 v
//
LOCUS       XP_077502426             241 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113309 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502426
VERSION     XP_077502426.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646300.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..241
                     /product="uncharacterized protein LOC144113309 isoform X2"
                     /calculated_mol_wt=25961
     CDS             1..241
                     /gene="LOC144113309"
                     /coded_by="XM_077646300.1:12..737"
                     /db_xref="GeneID:144113309"
ORIGIN      
        1 mtskapidta lskketmkrr vvslvkrgfp aplptpdpkt gaggrslkph rqvpgtgflr
       61 tfevsrpdtg prprldlgta phragtwssa gipwqheada rghlagatqp allllltraa
      121 eaapraswsq yvrlssrtqr ahsiaisiaa yctpadhapq wtsnlghrng akqhivshhv
      181 hghvwwkhis tvptvlllpt qnerkclcts gqcinspppt gssksciavn snhtkwatsk
      241 l
//
LOCUS       XP_077502427             586 aa            linear   INV 25-NOV-2025
DEFINITION  F-box and leucine-rich repeat protein 13-like [Amblyomma
            americanum].
ACCESSION   XP_077502427
VERSION     XP_077502427.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646301.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..586
                     /product="F-box and leucine-rich repeat protein 13-like"
                     /calculated_mol_wt=64857
     Region          13..50
                     /region_name="F-box-like"
                     /note="pfam12937"
                     /db_xref="CDD:463757"
     Region          75..98
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          99..112
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          124..149
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          158..183
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          232..257
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          258..281
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          284..309
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          <306..487
                     /region_name="AMN1"
                     /note="Antagonist of mitotic exit network protein 1;
                     cd09293"
                     /db_xref="CDD:187754"
     Region          310..362
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          363..387
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          388..413
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          414..449
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          448..>581
                     /region_name="AMN1"
                     /note="Antagonist of mitotic exit network protein 1;
                     cd09293"
                     /db_xref="CDD:187754"
     Region          450..474
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          475..500
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          501..526
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          527..550
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     CDS             1..586
                     /gene="LOC144113313"
                     /coded_by="XM_077646301.1:300..2060"
                     /db_xref="GeneID:144113313"
ORIGIN      
        1 melsedsdms rvlppellrr vfsflgvsdr lsagltcktw halatdgrly gdvafvletd
       61 vrermealaq tssvitwliv kntilytdte fwrhvgktlk klefescnfe pdefvsmire
      121 cpklehltis dcdsivlasg qsvldeatsg ipstmagina ldlsdnrfmt daafhqlita
      181 mgslstlsle gcelcghpav yrrfyppgag hstllmtpqd afqtllesga rlrvlnlnht
      241 mtddtylikl ckaysqtlqe lhlascaflg qrgvtalcln vprlkclnia rnrqiddgcl
      301 tticanlkel eeldisgcik itseglhals rlgklqhasf sncsgceqqg fvklfssrpw
      361 pgmrtldmss criddavvrc iaeqmpalvs ldvsqspllt dvavrtiwth lrllrvlrme
      421 rctnitdaal ygpdnsesce paslarltgl ktlslngcyq lsdagvaaal rfrelttldl
      481 ghcnlltams lkhvasrmps lsklvlsfcv rlddagvtll lwrlprvryi niegctgitd
      541 gllrnavrcr plkylnvnlc svtlkmirwa skerpdlelv htaqvr
//
LOCUS       XP_077502428             586 aa            linear   INV 25-NOV-2025
DEFINITION  F-box and leucine-rich repeat protein 13-like [Amblyomma
            americanum].
ACCESSION   XP_077502428
VERSION     XP_077502428.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646302.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..586
                     /product="F-box and leucine-rich repeat protein 13-like"
                     /calculated_mol_wt=64857
     Region          13..50
                     /region_name="F-box-like"
                     /note="pfam12937"
                     /db_xref="CDD:463757"
     Region          75..98
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          99..112
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          124..149
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          158..183
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          232..257
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          258..281
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          284..309
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          <306..487
                     /region_name="AMN1"
                     /note="Antagonist of mitotic exit network protein 1;
                     cd09293"
                     /db_xref="CDD:187754"
     Region          310..362
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          363..387
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          388..413
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          414..449
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          448..>581
                     /region_name="AMN1"
                     /note="Antagonist of mitotic exit network protein 1;
                     cd09293"
                     /db_xref="CDD:187754"
     Region          450..474
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          475..500
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          501..526
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          527..550
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     CDS             1..586
                     /gene="LOC144113313"
                     /coded_by="XM_077646302.1:280..2040"
                     /db_xref="GeneID:144113313"
ORIGIN      
        1 melsedsdms rvlppellrr vfsflgvsdr lsagltcktw halatdgrly gdvafvletd
       61 vrermealaq tssvitwliv kntilytdte fwrhvgktlk klefescnfe pdefvsmire
      121 cpklehltis dcdsivlasg qsvldeatsg ipstmagina ldlsdnrfmt daafhqlita
      181 mgslstlsle gcelcghpav yrrfyppgag hstllmtpqd afqtllesga rlrvlnlnht
      241 mtddtylikl ckaysqtlqe lhlascaflg qrgvtalcln vprlkclnia rnrqiddgcl
      301 tticanlkel eeldisgcik itseglhals rlgklqhasf sncsgceqqg fvklfssrpw
      361 pgmrtldmss criddavvrc iaeqmpalvs ldvsqspllt dvavrtiwth lrllrvlrme
      421 rctnitdaal ygpdnsesce paslarltgl ktlslngcyq lsdagvaaal rfrelttldl
      481 ghcnlltams lkhvasrmps lsklvlsfcv rlddagvtll lwrlprvryi niegctgitd
      541 gllrnavrcr plkylnvnlc svtlkmirwa skerpdlelv htaqvr
//
LOCUS       XP_077502429             212 aa            linear   INV 25-NOV-2025
DEFINITION  RAS oncogene family member Rab2 [Amblyomma americanum].
ACCESSION   XP_077502429
VERSION     XP_077502429.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646303.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..212
                     /product="RAS oncogene family member Rab2"
                     /calculated_mol_wt=23323
     Region          1..188
                     /region_name="PLN03108"
                     /note="Rab family protein; Provisional"
                     /db_xref="CDD:178655"
     CDS             1..212
                     /gene="Rab2"
                     /coded_by="XM_077646303.1:95..733"
                     /db_xref="GeneID:144113314"
ORIGIN      
        1 msyaylfkyi iigdtgvgks clllqftdkr fqpvhdltig vefgarmiti dgkqiklqiw
       61 dtagqeafrs itrsyyrgaa gallvyditr rdtfnhlttw ledarqhsns nmvimlignk
      121 sdletrrevk keegeafare hglifmetsa ktaanveeaf intakeiyek iqegvfdinn
      181 eangikigpq hsptspsmps sgggasasgg cc
//
LOCUS       XP_077502430             620 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113315 [Amblyomma americanum].
ACCESSION   XP_077502430
VERSION     XP_077502430.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646304.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..620
                     /product="uncharacterized protein LOC144113315"
                     /calculated_mol_wt=67817
     CDS             1..620
                     /gene="LOC144113315"
                     /coded_by="XM_077646304.1:500..2362"
                     /db_xref="GeneID:144113315"
ORIGIN      
        1 mdeggssrrs hrpvkhaskh kfrgrrrklt vktlptasep aaarpsdsda vssgefdaal
       61 eyvsasqkki glfddkervq deessvltdl talsllarga vcptcrrsem rvcesadkrk
      121 glasylelrc ensacpesvl satytsrrvs sggkasssva gdgprasray dsgssrdafa
      181 vnvktvvaar svgmgyeqlv rfsavlglpk pmhhksfsai trkvrdaams avsenlarsr
      241 qithseaggd eiavmyygtw hkrghkshng igtvvsldtg lcldfevlsn fclacsrhka
      301 lpnediwqaf hgpvcernvd csshameaea avriwrrsqs ydtplqfkkf lsdgdskaya
      361 avveanvygg aiekedctnh vakrlgtalr klkeplprge klkepaiqrl ttyyqvaits
      421 nrgsvkgmhq aiwasyfhsc ssdgasshky cpdgpaswck hrraealgea apahtplltk
      481 aqgkailpiy krltdeklla rcvqgktqna aeslnskiwl lcpktrfasr fvvematala
      541 vlwfnrghss fervleelgv lppqdlvglg ssrdsmrqkk msakqtaesr arrcmlrnks
      601 raddsackrr egetygagsf
//
LOCUS       XP_077502431            4127 aa            linear   INV 25-NOV-2025
DEFINITION  chromodomain helicase DNA binding protein kismet isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077502431
VERSION     XP_077502431.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646305.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..4127
                     /product="chromodomain helicase DNA binding protein kismet
                     isoform X1"
                     /calculated_mol_wt=449494
     Region          <5..>339
                     /region_name="Med15"
                     /note="ARC105 or Med15 subunit of Mediator complex
                     non-fungal; pfam09606"
                     /db_xref="CDD:312941"
     Region          <257..850
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1164..1231
                     /region_name="CD1_tandem_CHD5-9_like"
                     /note="repeat 1 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 5-9, and similar
                     proteins; cd18668"
                     /db_xref="CDD:349315"
     Site            order(1167,1199,1201..1202,1206)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:349315"
     Region          1250..>1272
                     /region_name="CD_CSD"
                     /note="CHROMO (CHRromatin Organization Modifier) domains
                     and chromo shadow domains; cl28914"
                     /db_xref="CDD:475127"
     Region          <1263..1533
                     /region_name="DUF612"
                     /note="Protein of unknown function, DUF612; pfam04747"
                     /db_xref="CDD:282585"
     Region          1434..1486
                     /region_name="CD_CSD"
                     /note="CHROMO (CHRromatin Organization Modifier) domains
                     and chromo shadow domains; cl28914"
                     /db_xref="CDD:475127"
     Region          1519..>2120
                     /region_name="PLN03142"
                     /note="Probable chromatin-remodeling complex ATPase chain;
                     Provisional"
                     /db_xref="CDD:215601"
     Region          <2829..3024
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          3724..3767
                     /region_name="BRK"
                     /note="BRK domain; pfam07533"
                     /db_xref="CDD:462196"
     CDS             1..4127
                     /gene="kis"
                     /coded_by="XM_077646305.1:598..12981"
                     /db_xref="GeneID:144113318"
ORIGIN      
        1 maeyqllggd gygqgmedgs gslagygaas qdlvsplmva npmgstvpgt sqmapysqmh
       61 pgypqpadqk mhyaaygagh vapeygarvh pmgeqqsami ggysqqsmvg qrmqasdqyp
      121 ygtmgapqrs qdmwrghymn sgagyqqhta dmgaqhtvtt atgvtsqhhr tsqgqygssq
      181 dyslhtpqqh gspqrppygp qgngthvaql tskaaaatat hmtdqsmtpy pqttypsaqt
      241 sanrstygta gamshsavaq smptartlrp ggyatsssss qapipqgspa rmqqypqsay
      301 spghpfsgsd atspshlspy ptqapnspqy rgsypsprrp tttppagsps vvtsqqsssq
      361 gsypspgath lsspgqvlsp gsgatasatp vassslqqle qmvmphmata kppptgtsst
      421 gsyygssvqq sygpaqaqqs lgygvvpsta qhhsypspnl msrsrplagt peassfgase
      481 vgpmaagsal parsdyamga tgpartapvs pasygaypqq qpvsslgyeq aqqqlqhlys
      541 vpqtaqtqkr iselqerlri lqqqqqpqqs allssvpaps tvvpgmgsal ggslgpmgsp
      601 tsmgaypsqy pqgaasgvpa apysqprqqm psaapaqqpq qlppaqqqph pqlppygpqa
      661 qqqllqnsql qpqplqaqsl qpqslqpqal qpqslqppal qpqtlqpqpv ppqslqpqtv
      721 qpqglqpqal qpqglqpqpq qqgpasvgma aasssvspqs ptvstlfpsm adsyrppsqp
      781 pslptpmisd qdlqsslppl plagvpsvvp avvddlcple gplsssldvp ytcplppedp
      841 yllppddvlp piepmtkrkp srskkkakts hempplglgp geglmppefm mppespgsmp
      901 lllpqppcme vppaaaakks kkrkpkepke geppkepkpk kpkkpkapkp pkepkeggap
      961 rrkskskkkn saeqapapqv ssteaqdpga pppevtaelp eggtiteata qaadaaaptd
     1021 eaktdaaaaq pveegktpkk kskekkekke pkapkeatpk tpkapkkklp rlslkfskkk
     1081 krkrlgssdk sdlegtppps peecdgvqkr rssrnthrkk ytddielqls gdegaldglh
     1141 iphtargqga etknvqvlvn vvtedtmvve kilasrmstr ksededkdsv eveeffvkyk
     1201 nlsyihcdwk tleelemtdk rvlqkvkrfr qkkdninsif dfledepfnp dyvevdrild
     1261 mtetvdpies eeanqsdvkd ekpdqieeia aegkekaepm eiepeeqngn gekecraqeq
     1321 tgedgdkepl aekeplidtc lpaeetkkep sggspktpsa paavtegpds gacggdgegs
     1381 pmehsikkel pdeeppssvk pededvfedn aavpiktdpl lapkventeq vkadssdgpn
     1441 gskgkvartt hkhylvkwrg lsyeestwel eedvdplkve hflrfkdppp kekwkvkkrp
     1501 kpsewkqide spvykggntl reyqleglsw ltfcwyngqn cilademglg ktiqsltfin
     1561 eivrygingp flviaplsti gnwqrefetw tdlnvityhg ssasrnmiqe yemyykdeng
     1621 qritdvfkfq vmittfeivl sdcmelqalp wracvideah rlknrnckll eglrmlnleh
     1681 svlltgtplq nnveelfsll nflepsrfss tetfmeefgd lktegqvdkl kallkpmmlr
     1741 rlkedveksl apkeetivev eltniqkkyy railernfaf ltkggvgtnv pnlmntmmel
     1801 rkccihpyli kgaeeqilqe yrlqhgdsld mtlnalvqas gklvlldkll pklkdgghrv
     1861 lvfsqmvrcl dlledylvhk rypyeridgr vrgnlrqaai drfckpdsdr fvfllctrag
     1921 glginltaad tviifdsdwn pqndlqaqar chrigqskav kvyrlicrnt yeremfdkas
     1981 lklgldravl qsmnsqkesl gvnqqltkqe iedllrkgay gaimdddgeg nnfceedidq
     2041 illrrthtit iesegkgstf skasftasst rsdieiddpn fwekwakkan ldvdelkgrn
     2101 elilqeprrr tqtkrfgtdd nmldlseles sdeddetgvr trgsrrsrhr dspviggrar
     2161 rggrasrgfs edeflgdiap gnwtrtecfq vekglltfgw grweesiaig lwrrrvtakd
     2221 vedisrvvll yclrhykgde kikgfiwdli aptedgetri hknhsglsap vprgrkgkkl
     2281 kngkgepdsp dmladwardd qynpdlllcd dgyrkhlhrh ankvllrvrl lyylkheiig
     2341 elhqqvfagv pardipippp tadgeppapw wdedadksll igvykhgyer fnlmrqdhtl
     2401 cflarcgppd gaallaemta npedledkre egkkdldeed pvspepstss eakppsepse
     2461 qavpstgeqp vpaaggpivp asqvpgelgt lefptpsdln trlrriitmy qrnhkkreli
     2521 naqrarntay qiitalqveg fnfvnrmerr ekfeaairer eirrletqqr wsrreeaefy
     2581 ravscygvdy rrqeqrfvwe gfrtvgrler krddtlteyf raframcrrv cgrklgeedd
     2641 nlqyvvepis eeratrclqr iellskiree ilphpelder lklcqpsmdl pdwwicgrhd
     2701 kdlligaaky glsrmdyhll ndgdlsfrev vraqsaskpp mvvvshhgps fplvsatvdt
     2761 avpatakatv dsnqevlcpe tvtqaavvls kdensdlpqv sevkvdnsce lkvdsssese
     2821 kvasecsgea akvtlsedhi tvtseknees tvqparddfk eeqarlpdvf padekmdvdk
     2881 piekspedee qtlkdsteen pgeemevdea ksemtseade dlevktleqt peegstkkqq
     2941 qiellevpli epkkieeedv edvpvqqnds dkvvaesaev ekaeigplng neakdeelhc
     3001 ekdvsgiaee shqeqptscn tttlapvssl smtcallelp tprvvlpppv eddnsktdve
     3061 ktdteqdpae mipcgskmpl tgfppfrvlt pqlteesqet nsqqddvlsq esfnldgegg
     3121 teppivhfmt hstrnpvrwp kdkvlqirle yivhavekke wpvprqgftl lpatptesps
     3181 spvassvasg seqtphatpe hiatpvvvre qhpppppppp ppsvllpdls arlspavvap
     3241 svaatatgia aattvsttve mapvvsvsag phspippaps ddllrgtrrr rrrrrfeiea
     3301 eraklralls hnlqqqqlkq lqqflapppp pstsslpstl atatapvstv apstppgaga
     3361 vaaaaaavaa attvgapspq vahreepskv pppppahqgs ssvrggstsa lgmldlrvka
     3421 aptpppakas tpappseepp mdlssggsap ttsnlsaaat mvpsvrsall ngsvaasrgr
     3481 gggrgrrsgg sridalalnl qarkqqqlre ehrqpgssad eredsdasst tsapalpppt
     3541 pmalpmsggk lhvdekeler llarksrhee ppkshkspav plslssmlss svlphgadvp
     3601 sssrqvrdip slssvatslp gankavdsrg gssalqqqqq qqqqqaaeqa saaaamhqdl
     3661 krwlddhpel mashpsltaa aaamafspmp slsshqlell elpesrrrgr rprldpskld
     3721 phcltgdenv svinrltgkk itgskapplk hlaewldknp mfdvdpkwaq lvkerrwlqh
     3781 skqneeaksq msqsasarrk rghlpempke rvaapeskrg rrtaasllss lhqaglggas
     3841 sssggaanlg fpstsalnpl sfsssmlgnf pglkffmepg kssaavtsps tssathplfl
     3901 pfgglagmgl anplfsfpgl gfqglpfeea ketkaqdger apskerhnkg spssssslpt
     3961 ssaslastsf pflypsglly nplglggfsl ptnmsasfas laqaglvngl gglgsvissa
     4021 askapsasla ssrrpaapsv tsalgaglrs svlppppass ttrrpapipp ptldsddesl
     4081 mgndmddldd lddrdnsdel deepdhkrpr eerpkkpeaa phdkvas
//
LOCUS       XP_077502432            4126 aa            linear   INV 25-NOV-2025
DEFINITION  chromodomain helicase DNA binding protein kismet isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077502432
VERSION     XP_077502432.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646306.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..4126
                     /product="chromodomain helicase DNA binding protein kismet
                     isoform X2"
                     /calculated_mol_wt=449366
     Region          <5..>339
                     /region_name="Med15"
                     /note="ARC105 or Med15 subunit of Mediator complex
                     non-fungal; pfam09606"
                     /db_xref="CDD:312941"
     Region          <257..874
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1163..1230
                     /region_name="CD1_tandem_CHD5-9_like"
                     /note="repeat 1 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 5-9, and similar
                     proteins; cd18668"
                     /db_xref="CDD:349315"
     Site            order(1166,1198,1200..1201,1205)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:349315"
     Region          1249..>1271
                     /region_name="CD_CSD"
                     /note="CHROMO (CHRromatin Organization Modifier) domains
                     and chromo shadow domains; cl28914"
                     /db_xref="CDD:475127"
     Region          <1262..1532
                     /region_name="DUF612"
                     /note="Protein of unknown function, DUF612; pfam04747"
                     /db_xref="CDD:282585"
     Region          1433..1485
                     /region_name="CD_CSD"
                     /note="CHROMO (CHRromatin Organization Modifier) domains
                     and chromo shadow domains; cl28914"
                     /db_xref="CDD:475127"
     Region          1518..>2119
                     /region_name="PLN03142"
                     /note="Probable chromatin-remodeling complex ATPase chain;
                     Provisional"
                     /db_xref="CDD:215601"
     Region          <2828..3023
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          3723..3766
                     /region_name="BRK"
                     /note="BRK domain; pfam07533"
                     /db_xref="CDD:462196"
     CDS             1..4126
                     /gene="kis"
                     /coded_by="XM_077646306.1:229..12609"
                     /db_xref="GeneID:144113318"
ORIGIN      
        1 maeyqllggd gygqgmedgs gslagygaas qdlvsplmva npmgstvpgt sqmapysqmh
       61 pgypqpadqk mhyaaygagh vapeygarvh pmgeqqsami ggysqqsmvg qrmqasdqyp
      121 ygtmgapqrs qdmwrghymn sgagyqqhta dmgaqhtvtt atgvtsqhhr tsqgqygssq
      181 dyslhtpqqh gspqrppygp qgngthvaql tskaaaatat hmtdqsmtpy pqttypsaqt
      241 sanrstygta gamshsavaq smptartlrp ggyatsssss qapipqgspa rmqqypqsay
      301 spghpfsgsd atspshlspy ptqapnspqy rgsypsprrp tttppagsps vvtsqqsssq
      361 gsypspgath lsspgqvlsp gsgatasatp vassslqqle qmvmphmata kppptgtsst
      421 gsyygssvqq sygpaqaqqs lgygvvpsta qhhsypspnl msrsrplagt peassfgase
      481 vgpmaagsal parsdyamga tgpartapvs pasygaypqq qpvsslgyeq aqqqlqhlys
      541 vpqtaqtqkr iselqerlri lqqqqqpqqs allssvpaps tvvpgmgsal ggslgpmgsp
      601 tsmgaypsqy pqgaasgvpa apysqprqqm psaapaqqpq qlppaqqphp qlppygpqaq
      661 qqllqnsqlq pqplqaqslq pqslqpqalq pqslqppalq pqtlqpqpvp pqslqpqtvq
      721 pqglqpqalq pqglqpqpqq qgpasvgmaa asssvspqsp tvstlfpsma dsyrppsqpp
      781 slptpmisdq dlqsslpplp lagvpsvvpa vvddlcpleg plsssldvpy tcplppedpy
      841 llppddvlpp iepmtkrkps rskkkaktsh empplglgpg eglmppefmm ppespgsmpl
      901 llpqppcmev ppaaaakksk krkpkepkeg eppkepkpkk pkkpkapkpp kepkeggapr
      961 rkskskkkns aeqapapqvs steaqdpgap ppevtaelpe ggtiteataq aadaaaptde
     1021 aktdaaaaqp veegktpkkk skekkekkep kapkeatpkt pkapkkklpr lslkfskkkk
     1081 rkrlgssdks dlegtpppsp eecdgvqkrr ssrnthrkky tddielqlsg degaldglhi
     1141 phtargqgae tknvqvlvnv vtedtmvvek ilasrmstrk sededkdsve veeffvkykn
     1201 lsyihcdwkt leelemtdkr vlqkvkrfrq kkdninsifd fledepfnpd yvevdrildm
     1261 tetvdpiese eanqsdvkde kpdqieeiaa egkekaepme iepeeqngng ekecraqeqt
     1321 gedgdkepla ekeplidtcl paeetkkeps ggspktpsap aavtegpdsg acggdgegsp
     1381 mehsikkelp deeppssvkp ededvfedna avpiktdpll apkventeqv kadssdgpng
     1441 skgkvartth khylvkwrgl syeestwele edvdplkveh flrfkdpppk ekwkvkkrpk
     1501 psewkqides pvykggntlr eyqleglswl tfcwyngqnc ilademglgk tiqsltfine
     1561 ivrygingpf lviaplstig nwqrefetwt dlnvityhgs sasrnmiqey emyykdengq
     1621 ritdvfkfqv mittfeivls dcmelqalpw racvideahr lknrncklle glrmlnlehs
     1681 vlltgtplqn nveelfslln flepsrfsst etfmeefgdl ktegqvdklk allkpmmlrr
     1741 lkedveksla pkeetiveve ltniqkkyyr ailernfafl tkggvgtnvp nlmntmmelr
     1801 kccihpylik gaeeqilqey rlqhgdsldm tlnalvqasg klvlldkllp klkdgghrvl
     1861 vfsqmvrcld lledylvhkr ypyeridgrv rgnlrqaaid rfckpdsdrf vfllctragg
     1921 lginltaadt viifdsdwnp qndlqaqarc hrigqskavk vyrlicrnty eremfdkasl
     1981 klgldravlq smnsqkeslg vnqqltkqei edllrkgayg aimdddgegn nfceedidqi
     2041 llrrthtiti esegkgstfs kasftasstr sdieiddpnf wekwakkanl dvdelkgrne
     2101 lilqeprrrt qtkrfgtddn mldlseless deddetgvrt rgsrrsrhrd spviggrarr
     2161 ggrasrgfse deflgdiapg nwtrtecfqv ekglltfgwg rweesiaigl wrrrvtakdv
     2221 edisrvvlly clrhykgdek ikgfiwdlia ptedgetrih knhsglsapv prgrkgkklk
     2281 ngkgepdspd mladwarddq ynpdlllcdd gyrkhlhrha nkvllrvrll yylkheiige
     2341 lhqqvfagvp ardipipppt adgeppapww dedadkslli gvykhgyerf nlmrqdhtlc
     2401 flarcgppdg aallaemtan pedledkree gkkdldeedp vspepstsse akppsepseq
     2461 avpstgeqpv paaggpivpa sqvpgelgtl efptpsdlnt rlrriitmyq rnhkkrelin
     2521 aqrarntayq iitalqvegf nfvnrmerre kfeaairere irrletqqrw srreeaefyr
     2581 avscygvdyr rqeqrfvweg frtvgrlerk rddtlteyfr aframcrrvc grklgeeddn
     2641 lqyvvepise eratrclqri ellskireei lphpelderl klcqpsmdlp dwwicgrhdk
     2701 dlligaakyg lsrmdyhlln dgdlsfrevv raqsaskppm vvvshhgpsf plvsatvdta
     2761 vpatakatvd snqevlcpet vtqaavvlsk densdlpqvs evkvdnscel kvdsssesek
     2821 vasecsgeaa kvtlsedhit vtsekneest vqparddfke eqarlpdvfp adekmdvdkp
     2881 iekspedeeq tlkdsteenp geemevdeak semtseaded levktleqtp eegstkkqqq
     2941 iellevplie pkkieeedve dvpvqqndsd kvvaesaeve kaeigplngn eakdeelhce
     3001 kdvsgiaees hqeqptscnt ttlapvssls mtcallelpt prvvlpppve ddnsktdvek
     3061 tdteqdpaem ipcgskmplt gfppfrvltp qlteesqetn sqqddvlsqe sfnldgeggt
     3121 eppivhfmth strnpvrwpk dkvlqirley ivhavekkew pvprqgftll patptespss
     3181 pvassvasgs eqtphatpeh iatpvvvreq hppppppppp psvllpdlsa rlspavvaps
     3241 vaatatgiaa attvsttvem apvvsvsagp hspippapsd dllrgtrrrr rrrrfeieae
     3301 raklrallsh nlqqqqlkql qqflappppp stsslpstla tatapvstva pstppgagav
     3361 aaaaaavaaa ttvgapspqv ahreepskvp ppppahqgss svrggstsal gmldlrvkaa
     3421 ptpppakast pappseeppm dlssggsapt tsnlsaaatm vpsvrsalln gsvaasrgrg
     3481 ggrgrrsggs ridalalnlq arkqqqlree hrqpgssade redsdasstt sapalppptp
     3541 malpmsggkl hvdekelerl larksrheep pkshkspavp lslssmlsss vlphgadvps
     3601 ssrqvrdips lssvatslpg ankavdsrgg ssalqqqqqq qqqqaaeqas aaaamhqdlk
     3661 rwlddhpelm ashpsltaaa aamafspmps lsshqlelle lpesrrrgrr prldpskldp
     3721 hcltgdenvs vinrltgkki tgskapplkh laewldknpm fdvdpkwaql vkerrwlqhs
     3781 kqneeaksqm sqsasarrkr ghlpempker vaapeskrgr rtaasllssl hqaglggass
     3841 ssggaanlgf pstsalnpls fsssmlgnfp glkffmepgk ssaavtspst ssathplflp
     3901 fgglagmgla nplfsfpglg fqglpfeeak etkaqdgera pskerhnkgs pssssslpts
     3961 saslastsfp flypsgllyn plglggfslp tnmsasfasl aqaglvnglg glgsvissaa
     4021 skapsaslas srrpaapsvt salgaglrss vlppppasst trrpapippp tldsddeslm
     4081 gndmddlddl ddrdnsdeld eepdhkrpre erpkkpeaap hdkvas
//
LOCUS       XP_077502433            4122 aa            linear   INV 25-NOV-2025
DEFINITION  chromodomain helicase DNA binding protein kismet isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077502433
VERSION     XP_077502433.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646307.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..4122
                     /product="chromodomain helicase DNA binding protein kismet
                     isoform X3"
                     /calculated_mol_wt=448917
     Region          <5..>339
                     /region_name="Med15"
                     /note="ARC105 or Med15 subunit of Mediator complex
                     non-fungal; pfam09606"
                     /db_xref="CDD:312941"
     Region          <257..850
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1164..1231
                     /region_name="CD1_tandem_CHD5-9_like"
                     /note="repeat 1 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 5-9, and similar
                     proteins; cd18668"
                     /db_xref="CDD:349315"
     Site            order(1167,1199,1201..1202,1206)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:349315"
     Region          1250..>1272
                     /region_name="CD_CSD"
                     /note="CHROMO (CHRromatin Organization Modifier) domains
                     and chromo shadow domains; cl28914"
                     /db_xref="CDD:475127"
     Region          <1263..1533
                     /region_name="DUF612"
                     /note="Protein of unknown function, DUF612; pfam04747"
                     /db_xref="CDD:282585"
     Region          1434..1486
                     /region_name="CD_CSD"
                     /note="CHROMO (CHRromatin Organization Modifier) domains
                     and chromo shadow domains; cl28914"
                     /db_xref="CDD:475127"
     Region          1519..>2120
                     /region_name="PLN03142"
                     /note="Probable chromatin-remodeling complex ATPase chain;
                     Provisional"
                     /db_xref="CDD:215601"
     Region          <2824..3019
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          3719..3762
                     /region_name="BRK"
                     /note="BRK domain; pfam07533"
                     /db_xref="CDD:462196"
     CDS             1..4122
                     /gene="kis"
                     /coded_by="XM_077646307.1:598..12966"
                     /db_xref="GeneID:144113318"
ORIGIN      
        1 maeyqllggd gygqgmedgs gslagygaas qdlvsplmva npmgstvpgt sqmapysqmh
       61 pgypqpadqk mhyaaygagh vapeygarvh pmgeqqsami ggysqqsmvg qrmqasdqyp
      121 ygtmgapqrs qdmwrghymn sgagyqqhta dmgaqhtvtt atgvtsqhhr tsqgqygssq
      181 dyslhtpqqh gspqrppygp qgngthvaql tskaaaatat hmtdqsmtpy pqttypsaqt
      241 sanrstygta gamshsavaq smptartlrp ggyatsssss qapipqgspa rmqqypqsay
      301 spghpfsgsd atspshlspy ptqapnspqy rgsypsprrp tttppagsps vvtsqqsssq
      361 gsypspgath lsspgqvlsp gsgatasatp vassslqqle qmvmphmata kppptgtsst
      421 gsyygssvqq sygpaqaqqs lgygvvpsta qhhsypspnl msrsrplagt peassfgase
      481 vgpmaagsal parsdyamga tgpartapvs pasygaypqq qpvsslgyeq aqqqlqhlys
      541 vpqtaqtqkr iselqerlri lqqqqqpqqs allssvpaps tvvpgmgsal ggslgpmgsp
      601 tsmgaypsqy pqgaasgvpa apysqprqqm psaapaqqpq qlppaqqqph pqlppygpqa
      661 qqqllqnsql qpqplqaqsl qpqslqpqal qpqslqppal qpqtlqpqpv ppqslqpqtv
      721 qpqglqpqal qpqglqpqpq qqgpasvgma aasssvspqs ptvstlfpsm adsyrppsqp
      781 pslptpmisd qdlqsslppl plagvpsvvp avvddlcple gplsssldvp ytcplppedp
      841 yllppddvlp piepmtkrkp srskkkakts hempplglgp geglmppefm mppespgsmp
      901 lllpqppcme vppaaaakks kkrkpkepke geppkepkpk kpkkpkapkp pkepkeggap
      961 rrkskskkkn saeqapapqv ssteaqdpga pppevtaelp eggtiteata qaadaaaptd
     1021 eaktdaaaaq pveegktpkk kskekkekke pkapkeatpk tpkapkkklp rlslkfskkk
     1081 krkrlgssdk sdlegtppps peecdgvqkr rssrnthrkk ytddielqls gdegaldglh
     1141 iphtargqga etknvqvlvn vvtedtmvve kilasrmstr ksededkdsv eveeffvkyk
     1201 nlsyihcdwk tleelemtdk rvlqkvkrfr qkkdninsif dfledepfnp dyvevdrild
     1261 mtetvdpies eeanqsdvkd ekpdqieeia aegkekaepm eiepeeqngn gekecraqeq
     1321 tgedgdkepl aekeplidtc lpaeetkkep sggspktpsa paavtegpds gacggdgegs
     1381 pmehsikkel pdeeppssvk pededvfedn aavpiktdpl lapkventeq vkadssdgpn
     1441 gskgkvartt hkhylvkwrg lsyeestwel eedvdplkve hflrfkdppp kekwkvkkrp
     1501 kpsewkqide spvykggntl reyqleglsw ltfcwyngqn cilademglg ktiqsltfin
     1561 eivrygingp flviaplsti gnwqrefetw tdlnvityhg ssasrnmiqe yemyykdeng
     1621 qritdvfkfq vmittfeivl sdcmelqalp wracvideah rlknrnckll eglrmlnleh
     1681 svlltgtplq nnveelfsll nflepsrfss tetfmeefgd lktegqvdkl kallkpmmlr
     1741 rlkedveksl apkeetivev eltniqkkyy railernfaf ltkggvgtnv pnlmntmmel
     1801 rkccihpyli kgaeeqilqe yrlqhgdsld mtlnalvqas gklvlldkll pklkdgghrv
     1861 lvfsqmvrcl dlledylvhk rypyeridgr vrgnlrqaai drfckpdsdr fvfllctrag
     1921 glginltaad tviifdsdwn pqndlqaqar chrigqskav kvyrlicrnt yeremfdkas
     1981 lklgldravl qsmnsqkesl gvnqqltkqe iedllrkgay gaimdddgeg nnfceedidq
     2041 illrrthtit iesegkgstf skasftasst rsdieiddpn fwekwakkan ldvdelkgrn
     2101 elilqeprrr tqtkrfgtdd nmldlseles sdeddetgvr trgsrrsrhr dspviggrar
     2161 rggrasrgfs edeflgdiap gnwtrtecfq vekglltfgw grweesiaig lwrrrvtakd
     2221 vedisrvvll yclrhykgde kikgfiwdli aptedgetri hknhsglsap vprgrkgkkl
     2281 kngkgepdsp dmladwardd qynpdlllcd dgyrkhlhrh ankvllrvrl lyylkheiig
     2341 elhqqvfagv pardipippp tadgeppapw wdedadksll igvykhgyer fnlmrqdhtl
     2401 cflarcgppd gaallaemta npedledkre egkkdldeed pvspepstss eakppsepse
     2461 qavpstgeqp vpaaggpivp asqvpgelgt lefptpsdln trlrriitmy qrnhkkreli
     2521 naqrariita lqvegfnfvn rmerrekfea airereirrl etqqrwsrre eaefyravsc
     2581 ygvdyrrqeq rfvwegfrtv grlerkrddt lteyfrafra mcrrvcgrkl geeddnlqyv
     2641 vepiseerat rclqriells kireeilphp elderlklcq psmdlpdwwi cgrhdkdlli
     2701 gaakyglsrm dyhllndgdl sfrevvraqs askppmvvvs hhgpsfplvs atvdtavpat
     2761 akatvdsnqe vlcpetvtqa avvlskdens dlpqvsevkv dnscelkvds ssesekvase
     2821 csgeaakvtl sedhitvtse kneestvqpa rddfkeeqar lpdvfpadek mdvdkpieks
     2881 pedeeqtlkd steenpgeem evdeaksemt seadedlevk tleqtpeegs tkkqqqiell
     2941 evpliepkki eeedvedvpv qqndsdkvva esaevekaei gplngneakd eelhcekdvs
     3001 giaeeshqeq ptscntttla pvsslsmtca llelptprvv lpppveddns ktdvektdte
     3061 qdpaemipcg skmpltgfpp frvltpqlte esqetnsqqd dvlsqesfnl dgeggteppi
     3121 vhfmthstrn pvrwpkdkvl qirleyivha vekkewpvpr qgftllpatp tespsspvas
     3181 svasgseqtp hatpehiatp vvvreqhppp pppppppsvl lpdlsarlsp avvapsvaat
     3241 atgiaaattv sttvemapvv svsagphspi ppapsddllr gtrrrrrrrr feieaerakl
     3301 rallshnlqq qqlkqlqqfl apppppstss lpstlatata pvstvapstp pgagavaaaa
     3361 aavaaattvg apspqvahre epskvppppp ahqgsssvrg gstsalgmld lrvkaaptpp
     3421 pakastpapp seeppmdlss ggsapttsnl saaatmvpsv rsallngsva asrgrgggrg
     3481 rrsggsrida lalnlqarkq qqlreehrqp gssadereds dassttsapa lppptpmalp
     3541 msggklhvde kelerllark srheeppksh kspavplsls smlsssvlph gadvpsssrq
     3601 vrdipslssv atslpganka vdsrggssal qqqqqqqqqq aaeqasaaaa mhqdlkrwld
     3661 dhpelmashp sltaaaaama fspmpslssh qlellelpes rrrgrrprld pskldphclt
     3721 gdenvsvinr ltgkkitgsk applkhlaew ldknpmfdvd pkwaqlvker rwlqhskqne
     3781 eaksqmsqsa sarrkrghlp empkervaap eskrgrrtaa sllsslhqag lggassssgg
     3841 aanlgfpsts alnplsfsss mlgnfpglkf fmepgkssaa vtspstssat hplflpfggl
     3901 agmglanplf sfpglgfqgl pfeeaketka qdgerapske rhnkgspsss sslptssasl
     3961 astsfpflyp sgllynplgl ggfslptnms asfaslaqag lvnglgglgs vissaaskap
     4021 saslassrrp aapsvtsalg aglrssvlpp ppassttrrp apippptlds ddeslmgndm
     4081 ddlddlddrd nsdeldeepd hkrpreerpk kpeaaphdkv as
//
LOCUS       XP_077502434            4113 aa            linear   INV 25-NOV-2025
DEFINITION  chromodomain helicase DNA binding protein kismet isoform X4
            [Amblyomma americanum].
ACCESSION   XP_077502434
VERSION     XP_077502434.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646308.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..4113
                     /product="chromodomain helicase DNA binding protein kismet
                     isoform X4"
                     /calculated_mol_wt=447948
     Region          <5..>339
                     /region_name="Med15"
                     /note="ARC105 or Med15 subunit of Mediator complex
                     non-fungal; pfam09606"
                     /db_xref="CDD:312941"
     Region          <257..850
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1164..1231
                     /region_name="CD1_tandem_CHD5-9_like"
                     /note="repeat 1 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 5-9, and similar
                     proteins; cd18668"
                     /db_xref="CDD:349315"
     Site            order(1167,1199,1201..1202,1206)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:349315"
     Region          1250..>1272
                     /region_name="CD_CSD"
                     /note="CHROMO (CHRromatin Organization Modifier) domains
                     and chromo shadow domains; cl28914"
                     /db_xref="CDD:475127"
     Region          1434..1486
                     /region_name="CD_CSD"
                     /note="CHROMO (CHRromatin Organization Modifier) domains
                     and chromo shadow domains; cl28914"
                     /db_xref="CDD:475127"
     Region          1519..>2120
                     /region_name="PLN03142"
                     /note="Probable chromatin-remodeling complex ATPase chain;
                     Provisional"
                     /db_xref="CDD:215601"
     Region          <2815..3010
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          3710..3753
                     /region_name="BRK"
                     /note="BRK domain; pfam07533"
                     /db_xref="CDD:462196"
     CDS             1..4113
                     /gene="kis"
                     /coded_by="XM_077646308.1:598..12939"
                     /db_xref="GeneID:144113318"
ORIGIN      
        1 maeyqllggd gygqgmedgs gslagygaas qdlvsplmva npmgstvpgt sqmapysqmh
       61 pgypqpadqk mhyaaygagh vapeygarvh pmgeqqsami ggysqqsmvg qrmqasdqyp
      121 ygtmgapqrs qdmwrghymn sgagyqqhta dmgaqhtvtt atgvtsqhhr tsqgqygssq
      181 dyslhtpqqh gspqrppygp qgngthvaql tskaaaatat hmtdqsmtpy pqttypsaqt
      241 sanrstygta gamshsavaq smptartlrp ggyatsssss qapipqgspa rmqqypqsay
      301 spghpfsgsd atspshlspy ptqapnspqy rgsypsprrp tttppagsps vvtsqqsssq
      361 gsypspgath lsspgqvlsp gsgatasatp vassslqqle qmvmphmata kppptgtsst
      421 gsyygssvqq sygpaqaqqs lgygvvpsta qhhsypspnl msrsrplagt peassfgase
      481 vgpmaagsal parsdyamga tgpartapvs pasygaypqq qpvsslgyeq aqqqlqhlys
      541 vpqtaqtqkr iselqerlri lqqqqqpqqs allssvpaps tvvpgmgsal ggslgpmgsp
      601 tsmgaypsqy pqgaasgvpa apysqprqqm psaapaqqpq qlppaqqqph pqlppygpqa
      661 qqqllqnsql qpqplqaqsl qpqslqpqal qpqslqppal qpqtlqpqpv ppqslqpqtv
      721 qpqglqpqal qpqglqpqpq qqgpasvgma aasssvspqs ptvstlfpsm adsyrppsqp
      781 pslptpmisd qdlqsslppl plagvpsvvp avvddlcple gplsssldvp ytcplppedp
      841 yllppddvlp piepmtkrkp srskkkakts hempplglgp geglmppefm mppespgsmp
      901 lllpqppcme vppaaaakks kkrkpkepke geppkepkpk kpkkpkapkp pkepkeggap
      961 rrkskskkkn saeqapapqv ssteaqdpga pppevtaelp eggtiteata qaadaaaptd
     1021 eaktdaaaaq pveegktpkk kskekkekke pkapkeatpk tpkapkkklp rlslkfskkk
     1081 krkrlgssdk sdlegtppps peecdgvqkr rssrnthrkk ytddielqls gdegaldglh
     1141 iphtargqga etknvqvlvn vvtedtmvve kilasrmstr ksededkdsv eveeffvkyk
     1201 nlsyihcdwk tleelemtdk rvlqkvkrfr qkkdninsif dfledepfnp dyvevdrild
     1261 mtetvdpies eeanqsdvkd ekpdqieeia aegkekaepm eiepeeqngn gekecraqeq
     1321 tgedgdkepl aekeplidtc lpaeetkkep sggspktpsa paavtegpds gacggdgegs
     1381 pmehsikkel pdeeppssvk pededvfedn aavpiktdpl lapkventeq vkadssdgpn
     1441 gskgkvartt hkhylvkwrg lsyeestwel eedvdplkve hflrfkdppp kekwkvkkrp
     1501 kpsewkqide spvykggntl reyqleglsw ltfcwyngqn cilademglg ktiqsltfin
     1561 eivrygingp flviaplsti gnwqrefetw tdlnvityhg ssasrnmiqe yemyykdeng
     1621 qritdvfkfq vmittfeivl sdcmelqalp wracvideah rlknrnckll eglrmlnleh
     1681 svlltgtplq nnveelfsll nflepsrfss tetfmeefgd lktegqvdkl kallkpmmlr
     1741 rlkedveksl apkeetivev eltniqkkyy railernfaf ltkggvgtnv pnlmntmmel
     1801 rkccihpyli kgaeeqilqe yrlqhgdsld mtlnalvqas gklvlldkll pklkdgghrv
     1861 lvfsqmvrcl dlledylvhk rypyeridgr vrgnlrqaai drfckpdsdr fvfllctrag
     1921 glginltaad tviifdsdwn pqndlqaqar chrigqskav kvyrlicrnt yeremfdkas
     1981 lklgldravl qsmnsqkesl gvnqqltkqe iedllrkgay gaimdddgeg nnfceedidq
     2041 illrrthtit iesegkgstf skasftasst rsdieiddpn fwekwakkan ldvdelkgrn
     2101 elilqeprrr tqtkrfgtdd nmldlseles sdeddetgvr trgsrrsrhr dspviggrar
     2161 rggrasrgfs edeflgdiap gnwtrtecfq vekglltfgw grweesiaig lwrrrvtakd
     2221 vedisrvvll yclrhykgde kikgfiwdli aptedgetri hknhsglsap vprgrkgkkl
     2281 kngkgepdsp dmladwardd qynpdlllcd dgyrkhlhrh ankvllrvrl lyylkheiig
     2341 elhqqvfagv pardipippp tadgeppapw wdedadksll igvykhgyer fnlmrqdhtl
     2401 cflarcgppd gaallaemta npedledkre egkkdldeed pvspepstss eakppsepse
     2461 qavpstgeqp vpaaggpivp asqvpgelgt lefptpsdln trlrriitmy qrnhkkreli
     2521 naqrarntay qrmerrekfe aairereirr letqqrwsrr eeaefyravs cygvdyrrqe
     2581 qrfvwegfrt vgrlerkrdd tlteyfrafr amcrrvcgrk lgeeddnlqy vvepiseera
     2641 trclqriell skireeilph pelderlklc qpsmdlpdww icgrhdkdll igaakyglsr
     2701 mdyhllndgd lsfrevvraq saskppmvvv shhgpsfplv satvdtavpa takatvdsnq
     2761 evlcpetvtq aavvlskden sdlpqvsevk vdnscelkvd sssesekvas ecsgeaakvt
     2821 lsedhitvts ekneestvqp arddfkeeqa rlpdvfpade kmdvdkpiek spedeeqtlk
     2881 dsteenpgee mevdeaksem tseadedlev ktleqtpeeg stkkqqqiel levpliepkk
     2941 ieeedvedvp vqqndsdkvv aesaevekae igplngneak deelhcekdv sgiaeeshqe
     3001 qptscntttl apvsslsmtc allelptprv vlpppveddn sktdvektdt eqdpaemipc
     3061 gskmpltgfp pfrvltpqlt eesqetnsqq ddvlsqesfn ldgeggtepp ivhfmthstr
     3121 npvrwpkdkv lqirleyivh avekkewpvp rqgftllpat ptespsspva ssvasgseqt
     3181 phatpehiat pvvvreqhpp ppppppppsv llpdlsarls pavvapsvaa tatgiaaatt
     3241 vsttvemapv vsvsagphsp ippapsddll rgtrrrrrrr rfeieaerak lrallshnlq
     3301 qqqlkqlqqf lapppppsts slpstlatat apvstvapst ppgagavaaa aaavaaattv
     3361 gapspqvahr eepskvpppp pahqgsssvr ggstsalgml dlrvkaaptp ppakastpap
     3421 pseeppmdls sggsapttsn lsaaatmvps vrsallngsv aasrgrgggr grrsggsrid
     3481 alalnlqark qqqlreehrq pgssadered sdassttsap alppptpmal pmsggklhvd
     3541 ekelerllar ksrheeppks hkspavplsl ssmlsssvlp hgadvpsssr qvrdipslss
     3601 vatslpgank avdsrggssa lqqqqqqqqq qaaeqasaaa amhqdlkrwl ddhpelmash
     3661 psltaaaaam afspmpslss hqlellelpe srrrgrrprl dpskldphcl tgdenvsvin
     3721 rltgkkitgs kapplkhlae wldknpmfdv dpkwaqlvke rrwlqhskqn eeaksqmsqs
     3781 asarrkrghl pempkervaa peskrgrrta asllsslhqa glggassssg gaanlgfpst
     3841 salnplsfss smlgnfpglk ffmepgkssa avtspstssa thplflpfgg lagmglanpl
     3901 fsfpglgfqg lpfeeaketk aqdgerapsk erhnkgspss ssslptssas lastsfpfly
     3961 psgllynplg lggfslptnm sasfaslaqa glvnglgglg svissaaska psaslassrr
     4021 paapsvtsal gaglrssvlp pppassttrr papippptld sddeslmgnd mddlddlddr
     4081 dnsdeldeep dhkrpreerp kkpeaaphdk vas
//
LOCUS       XP_077502435            4108 aa            linear   INV 25-NOV-2025
DEFINITION  chromodomain helicase DNA binding protein kismet isoform X5
            [Amblyomma americanum].
ACCESSION   XP_077502435
VERSION     XP_077502435.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646309.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..4108
                     /product="chromodomain helicase DNA binding protein kismet
                     isoform X5"
                     /calculated_mol_wt=447370
     Region          <5..>339
                     /region_name="Med15"
                     /note="ARC105 or Med15 subunit of Mediator complex
                     non-fungal; pfam09606"
                     /db_xref="CDD:312941"
     Region          <257..850
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1164..1231
                     /region_name="CD1_tandem_CHD5-9_like"
                     /note="repeat 1 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 5-9, and similar
                     proteins; cd18668"
                     /db_xref="CDD:349315"
     Site            order(1167,1199,1201..1202,1206)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:349315"
     Region          1250..>1272
                     /region_name="CD_CSD"
                     /note="CHROMO (CHRromatin Organization Modifier) domains
                     and chromo shadow domains; cl28914"
                     /db_xref="CDD:475127"
     Region          1434..1486
                     /region_name="CD_CSD"
                     /note="CHROMO (CHRromatin Organization Modifier) domains
                     and chromo shadow domains; cl28914"
                     /db_xref="CDD:475127"
     Region          1519..>2120
                     /region_name="PLN03142"
                     /note="Probable chromatin-remodeling complex ATPase chain;
                     Provisional"
                     /db_xref="CDD:215601"
     Region          <2810..3005
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          3705..3748
                     /region_name="BRK"
                     /note="BRK domain; pfam07533"
                     /db_xref="CDD:462196"
     CDS             1..4108
                     /gene="kis"
                     /coded_by="XM_077646309.1:598..12924"
                     /db_xref="GeneID:144113318"
ORIGIN      
        1 maeyqllggd gygqgmedgs gslagygaas qdlvsplmva npmgstvpgt sqmapysqmh
       61 pgypqpadqk mhyaaygagh vapeygarvh pmgeqqsami ggysqqsmvg qrmqasdqyp
      121 ygtmgapqrs qdmwrghymn sgagyqqhta dmgaqhtvtt atgvtsqhhr tsqgqygssq
      181 dyslhtpqqh gspqrppygp qgngthvaql tskaaaatat hmtdqsmtpy pqttypsaqt
      241 sanrstygta gamshsavaq smptartlrp ggyatsssss qapipqgspa rmqqypqsay
      301 spghpfsgsd atspshlspy ptqapnspqy rgsypsprrp tttppagsps vvtsqqsssq
      361 gsypspgath lsspgqvlsp gsgatasatp vassslqqle qmvmphmata kppptgtsst
      421 gsyygssvqq sygpaqaqqs lgygvvpsta qhhsypspnl msrsrplagt peassfgase
      481 vgpmaagsal parsdyamga tgpartapvs pasygaypqq qpvsslgyeq aqqqlqhlys
      541 vpqtaqtqkr iselqerlri lqqqqqpqqs allssvpaps tvvpgmgsal ggslgpmgsp
      601 tsmgaypsqy pqgaasgvpa apysqprqqm psaapaqqpq qlppaqqqph pqlppygpqa
      661 qqqllqnsql qpqplqaqsl qpqslqpqal qpqslqppal qpqtlqpqpv ppqslqpqtv
      721 qpqglqpqal qpqglqpqpq qqgpasvgma aasssvspqs ptvstlfpsm adsyrppsqp
      781 pslptpmisd qdlqsslppl plagvpsvvp avvddlcple gplsssldvp ytcplppedp
      841 yllppddvlp piepmtkrkp srskkkakts hempplglgp geglmppefm mppespgsmp
      901 lllpqppcme vppaaaakks kkrkpkepke geppkepkpk kpkkpkapkp pkepkeggap
      961 rrkskskkkn saeqapapqv ssteaqdpga pppevtaelp eggtiteata qaadaaaptd
     1021 eaktdaaaaq pveegktpkk kskekkekke pkapkeatpk tpkapkkklp rlslkfskkk
     1081 krkrlgssdk sdlegtppps peecdgvqkr rssrnthrkk ytddielqls gdegaldglh
     1141 iphtargqga etknvqvlvn vvtedtmvve kilasrmstr ksededkdsv eveeffvkyk
     1201 nlsyihcdwk tleelemtdk rvlqkvkrfr qkkdninsif dfledepfnp dyvevdrild
     1261 mtetvdpies eeanqsdvkd ekpdqieeia aegkekaepm eiepeeqngn gekecraqeq
     1321 tgedgdkepl aekeplidtc lpaeetkkep sggspktpsa paavtegpds gacggdgegs
     1381 pmehsikkel pdeeppssvk pededvfedn aavpiktdpl lapkventeq vkadssdgpn
     1441 gskgkvartt hkhylvkwrg lsyeestwel eedvdplkve hflrfkdppp kekwkvkkrp
     1501 kpsewkqide spvykggntl reyqleglsw ltfcwyngqn cilademglg ktiqsltfin
     1561 eivrygingp flviaplsti gnwqrefetw tdlnvityhg ssasrnmiqe yemyykdeng
     1621 qritdvfkfq vmittfeivl sdcmelqalp wracvideah rlknrnckll eglrmlnleh
     1681 svlltgtplq nnveelfsll nflepsrfss tetfmeefgd lktegqvdkl kallkpmmlr
     1741 rlkedveksl apkeetivev eltniqkkyy railernfaf ltkggvgtnv pnlmntmmel
     1801 rkccihpyli kgaeeqilqe yrlqhgdsld mtlnalvqas gklvlldkll pklkdgghrv
     1861 lvfsqmvrcl dlledylvhk rypyeridgr vrgnlrqaai drfckpdsdr fvfllctrag
     1921 glginltaad tviifdsdwn pqndlqaqar chrigqskav kvyrlicrnt yeremfdkas
     1981 lklgldravl qsmnsqkesl gvnqqltkqe iedllrkgay gaimdddgeg nnfceedidq
     2041 illrrthtit iesegkgstf skasftasst rsdieiddpn fwekwakkan ldvdelkgrn
     2101 elilqeprrr tqtkrfgtdd nmldlseles sdeddetgvr trgsrrsrhr dspviggrar
     2161 rggrasrgfs edeflgdiap gnwtrtecfq vekglltfgw grweesiaig lwrrrvtakd
     2221 vedisrvvll yclrhykgde kikgfiwdli aptedgetri hknhsglsap vprgrkgkkl
     2281 kngkgepdsp dmladwardd qynpdlllcd dgyrkhlhrh ankvllrvrl lyylkheiig
     2341 elhqqvfagv pardipippp tadgeppapw wdedadksll igvykhgyer fnlmrqdhtl
     2401 cflarcgppd gaallaemta npedledkre egkkdldeed pvspepstss eakppsepse
     2461 qavpstgeqp vpaaggpivp asqvpgelgt lefptpsdln trlrriitmy qrnhkkreli
     2521 naqrarrmer rekfeaaire reirrletqq rwsrreeaef yravscygvd yrrqeqrfvw
     2581 egfrtvgrle rkrddtltey fraframcrr vcgrklgeed dnlqyvvepi seeratrclq
     2641 riellskire eilphpelde rlklcqpsmd lpdwwicgrh dkdlligaak yglsrmdyhl
     2701 lndgdlsfre vvraqsaskp pmvvvshhgp sfplvsatvd tavpatakat vdsnqevlcp
     2761 etvtqaavvl skdensdlpq vsevkvdnsc elkvdssses ekvasecsge aakvtlsedh
     2821 itvtseknee stvqparddf keeqarlpdv fpadekmdvd kpiekspede eqtlkdstee
     2881 npgeemevde aksemtsead edlevktleq tpeegstkkq qqiellevpl iepkkieeed
     2941 vedvpvqqnd sdkvvaesae vekaeigpln gneakdeelh cekdvsgiae eshqeqptsc
     3001 ntttlapvss lsmtcallel ptprvvlppp veddnsktdv ektdteqdpa emipcgskmp
     3061 ltgfppfrvl tpqlteesqe tnsqqddvls qesfnldgeg gteppivhfm thstrnpvrw
     3121 pkdkvlqirl eyivhavekk ewpvprqgft llpatptesp sspvassvas gseqtphatp
     3181 ehiatpvvvr eqhppppppp pppsvllpdl sarlspavva psvaatatgi aaattvsttv
     3241 emapvvsvsa gphspippap sddllrgtrr rrrrrrfeie aeraklrall shnlqqqqlk
     3301 qlqqflappp ppstsslpst latatapvst vapstppgag avaaaaaava aattvgapsp
     3361 qvahreepsk vpppppahqg sssvrggsts algmldlrvk aaptpppaka stpappseep
     3421 pmdlssggsa pttsnlsaaa tmvpsvrsal lngsvaasrg rgggrgrrsg gsridalaln
     3481 lqarkqqqlr eehrqpgssa deredsdass ttsapalppp tpmalpmsgg klhvdekele
     3541 rllarksrhe eppkshkspa vplslssmls ssvlphgadv psssrqvrdi pslssvatsl
     3601 pgankavdsr ggssalqqqq qqqqqqaaeq asaaaamhqd lkrwlddhpe lmashpslta
     3661 aaaamafspm pslsshqlel lelpesrrrg rrprldpskl dphcltgden vsvinrltgk
     3721 kitgskappl khlaewldkn pmfdvdpkwa qlvkerrwlq hskqneeaks qmsqsasarr
     3781 krghlpempk ervaapeskr grrtaaslls slhqaglgga ssssggaanl gfpstsalnp
     3841 lsfsssmlgn fpglkffmep gkssaavtsp stssathplf lpfgglagmg lanplfsfpg
     3901 lgfqglpfee aketkaqdge rapskerhnk gspssssslp tssaslasts fpflypsgll
     3961 ynplglggfs lptnmsasfa slaqaglvng lgglgsviss aaskapsasl assrrpaaps
     4021 vtsalgaglr ssvlppppas sttrrpapip pptldsddes lmgndmddld dlddrdnsde
     4081 ldeepdhkrp reerpkkpea aphdkvas
//
LOCUS       XP_077502437            2823 aa            linear   INV 25-NOV-2025
DEFINITION  C-type lectin domain-containing protein bark beetle isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077502437
VERSION     XP_077502437.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646311.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2823
                     /product="C-type lectin domain-containing protein bark
                     beetle isoform X1"
                     /calculated_mol_wt=318397
     Region          117..220
                     /region_name="SR"
                     /note="Scavenger receptor Cys-rich; smart00202"
                     /db_xref="CDD:214555"
     Region          <277..363
                     /region_name="Beta_helix"
                     /note="Right handed beta helix region; pfam13229"
                     /db_xref="CDD:463811"
     Region          <496..607
                     /region_name="Beta_helix"
                     /note="Right handed beta helix region; pfam13229"
                     /db_xref="CDD:463811"
     Region          982..1083
                     /region_name="SR"
                     /note="Scavenger receptor Cys-rich; smart00202"
                     /db_xref="CDD:214555"
     Region          <1129..>1200
                     /region_name="Beta_helix"
                     /note="Right handed beta helix region; pfam13229"
                     /db_xref="CDD:463811"
     Region          1394..1554
                     /region_name="Beta_helix"
                     /note="Right handed beta helix region; pfam13229"
                     /db_xref="CDD:463811"
     Region          1806..1903
                     /region_name="SRCR"
                     /note="Scavenger receptor cysteine-rich domain; pfam00530"
                     /db_xref="CDD:459844"
     Region          2477..2574
                     /region_name="CLECT"
                     /note="C-type lectin (CTL) or carbohydrate-recognition
                     domain (CRD); smart00034"
                     /db_xref="CDD:214480"
     Site            order(2550,2554,2556,2560,2562..2564,2567..2569)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     CDS             1..2823
                     /gene="bark"
                     /coded_by="XM_077646311.1:179..8650"
                     /db_xref="GeneID:144113319"
ORIGIN      
        1 mrhrpawasa vllcwlmcgs lgggrpveir drqvfnlsgs pyrmardivv edtgelviep
       61 gvelrfapgv gilvngvlka qgtaqrpvtl tvlneseprr ephsvvhrdt svewpsvrls
      121 dgpgplrgrl qmrhgahwws vctraknwtn edvrvvcrhl gfsggllqgw lprhndsrql
      181 mfaspncigd eqsldrcpgw ahrqlgsgic dfqldievac eptlqeqarh ywrglhfela
      241 ktelqqkqis pndwintevs qsilqhvvmk yagrqqdgna taaiqvygtp pvmknlevrl
      301 sayhginvtm pnqpveiqqs vfsenrgygi fintskggvn lsgvrvmkng adgivytrhe
      361 tkigsetsfc esssvsniry ptvfvyqldr rnyksscfrv lpagygdlvl tvhfkdmqry
      421 dgrkkleggt vtvwngkqkl dehklaqfti dnttvpqsvs sekgtpltie fqppkytysh
      481 qndifpqlqf smeavlgvgk dydlnvtdch isnnngrgiw lrdgvagasi veteiqgnth
      541 vagihidggv gdllvnrsdi sgnqgdginv thaggykhiq qsfihgnsrr gialwfnets
      601 nwasfnftth isfsnissng digvllgnvc radafsnism ntfganggpa vvyqscwvks
      661 tvngeakvda lhithnrfgg stklsvqmtp alninatikh nsflnhtsga lwienkdhge
      721 hlehhadkva eievsendfk fnsgtfvaqi glvhgssvqi lnfhhnklee nsirqpfarl
      781 nprskvaavv avtssntvie rnrfsnprsd yelgshlddh gsiinaslnf wgldtytndv
      841 svriynrifd rkdrynlapv qflqyllngq dldtkeqiss nferdkimqf qtagnkeigg
      901 evmgdvtler gdytvkkdif vrrgskllik pgtrlqfeqs igmmvegrle sdgtseqivy
      961 talsvpmead etetlptemp slrlssvteg tievtvngtt ggvcaygfti eaaalvcqql
     1021 gmvlsshdwl mepasqassg gtshilfsnv rcsemdtdfl tcrhevlged fenscfsevg
     1081 ircirpswag irlgvhaets ilknvvieka gqldyathvf kpalqvdfnn hileklnire
     1141 ntdsgiglmw ndifrdihnp vvkssevynn nrhgiltrsq gvditenriq gnrgggvwyd
     1201 pvftkreqrd avrwaedtat visdnirlnp derhyirvae aspntrrefr vscedkraig
     1261 imvvdpired stetllirrg qdfedtsapm wdirknltsf plretgfivt lryetgsapk
     1321 ggallmmtav qplgarrmpc tgffckdtgq aqmleyklrl niksniiegc gwgfgsnhyn
     1381 rdiapdeafy yhrysnetvh lvkntitesr qhavhvsspl wdpltsslae ivynltenri
     1441 nnnqgginhy srdlrssnnl fhwiinrtef snnnggidlr lpyvwdynen ythsmaivnc
     1501 tfknnrnfkl ridghfarfn atwniiedne chsgegvftv agmekemlit rntfrrnrgk
     1561 yvvefklesh serfsivgan fyynmlqnnq pdassgvspd hyepnsyavs irgvqrinit
     1621 qnflvnpdhe yeflagvfts slnnavnvae nwwgslnatd iksrvfdldd wngyaaadfs
     1681 pylargefgg ptlsydgsde wgfrmdtsrp lggrlfksit lyqrsspyii rsdltilpna
     1741 tltilegvel qfypsvgilv lgnliavgrp wapirmgplk qlqqsfrykr aspkrfvrlc
     1801 vdqacsadrr dgfleffnst tlqwvpvcdq rfternaevv crelgfgtld vwidrgyrhe
     1861 lgptelsiin swlhplqctg hepslsscef rlngygehsy acspfdhkfv yihcgeeltt
     1921 peeefwggir fsvpdykaqn smgggvyass ryytpptsal rhvhirkagi lhnekgpavq
     1981 vvlrdvtleh lnisecasng idiiaspgyq yihavnvthn rgtginfvtl ngqtstaerl
     2041 nyiplgpvdi pynvfglvdi cdnnkafpvd gkvllyykyd nrpvecikif tsksrlsqkv
     2101 gfrilqfnlf nsteftpypd sisifdgdvf nstvkkighm svgtdskest aqttfyrsqg
     2161 itlsvklhat gasglngfia evvtiplqti tardskhnit asyfnknrrg aityrsagei
     2221 tpiltlhgnr fedngdalfg nfssseaavf fdiqnsmemh ilrnlfiynq gglrifvsst
     2281 nsvsalkgyv hnnlftqnkn reslyvrsge tgmyqylhvy rnyfaresaa fhdnvvfekv
     2341 tfnfsenmiv esegyrqmsv lgfektqssg qlvyrnwlwd neafakelka tvlaessgqk
     2401 fsknyflnrl nyfemvtann teqenssyvd aennwwgfke tsavvgriyd rsdqpellev
     2461 rfqphleynd tvisgncmgg wemiedtcfl yipgpmthae akrfcqldas smpylkqklt
     2521 klmqyistwq edfdwlyeri wvqsldvpld qcavlyrggv wahdcndrlp flceraqeiv
     2581 vrsdywyqep vsitamsllg vllicvciil gfwwcksqer hkqhlqrqns irasirsasn
     2641 rsldrdaffs dykhrieral adtptstppr lavhppkhng stdsvakqyt ydlddsrsyd
     2701 dtvhevrnpa ldyssdieek yptsvpgtrk gaadlqhptf dltyenrgym drsvggydds
     2761 ldasrdwssg tdstldmkrs letpqavpmd psdntfkape pyqpshyant dnyyrrqple
     2821 tam
//
LOCUS       XP_077502438            2808 aa            linear   INV 25-NOV-2025
DEFINITION  C-type lectin domain-containing protein bark beetle isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077502438
VERSION     XP_077502438.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646312.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2808
                     /product="C-type lectin domain-containing protein bark
                     beetle isoform X2"
                     /calculated_mol_wt=316725
     Region          117..220
                     /region_name="SR"
                     /note="Scavenger receptor Cys-rich; smart00202"
                     /db_xref="CDD:214555"
     Region          <277..363
                     /region_name="Beta_helix"
                     /note="Right handed beta helix region; pfam13229"
                     /db_xref="CDD:463811"
     Region          <496..607
                     /region_name="Beta_helix"
                     /note="Right handed beta helix region; pfam13229"
                     /db_xref="CDD:463811"
     Region          982..1083
                     /region_name="SR"
                     /note="Scavenger receptor Cys-rich; smart00202"
                     /db_xref="CDD:214555"
     Region          <1129..>1200
                     /region_name="Beta_helix"
                     /note="Right handed beta helix region; pfam13229"
                     /db_xref="CDD:463811"
     Region          1379..1539
                     /region_name="Beta_helix"
                     /note="Right handed beta helix region; pfam13229"
                     /db_xref="CDD:463811"
     Region          1791..1888
                     /region_name="SRCR"
                     /note="Scavenger receptor cysteine-rich domain; pfam00530"
                     /db_xref="CDD:459844"
     Region          2462..2559
                     /region_name="CLECT"
                     /note="C-type lectin (CTL) or carbohydrate-recognition
                     domain (CRD); smart00034"
                     /db_xref="CDD:214480"
     Site            order(2535,2539,2541,2545,2547..2549,2552..2554)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     CDS             1..2808
                     /gene="bark"
                     /coded_by="XM_077646312.1:179..8605"
                     /db_xref="GeneID:144113319"
ORIGIN      
        1 mrhrpawasa vllcwlmcgs lgggrpveir drqvfnlsgs pyrmardivv edtgelviep
       61 gvelrfapgv gilvngvlka qgtaqrpvtl tvlneseprr ephsvvhrdt svewpsvrls
      121 dgpgplrgrl qmrhgahwws vctraknwtn edvrvvcrhl gfsggllqgw lprhndsrql
      181 mfaspncigd eqsldrcpgw ahrqlgsgic dfqldievac eptlqeqarh ywrglhfela
      241 ktelqqkqis pndwintevs qsilqhvvmk yagrqqdgna taaiqvygtp pvmknlevrl
      301 sayhginvtm pnqpveiqqs vfsenrgygi fintskggvn lsgvrvmkng adgivytrhe
      361 tkigsetsfc esssvsniry ptvfvyqldr rnyksscfrv lpagygdlvl tvhfkdmqry
      421 dgrkkleggt vtvwngkqkl dehklaqfti dnttvpqsvs sekgtpltie fqppkytysh
      481 qndifpqlqf smeavlgvgk dydlnvtdch isnnngrgiw lrdgvagasi veteiqgnth
      541 vagihidggv gdllvnrsdi sgnqgdginv thaggykhiq qsfihgnsrr gialwfnets
      601 nwasfnftth isfsnissng digvllgnvc radafsnism ntfganggpa vvyqscwvks
      661 tvngeakvda lhithnrfgg stklsvqmtp alninatikh nsflnhtsga lwienkdhge
      721 hlehhadkva eievsendfk fnsgtfvaqi glvhgssvqi lnfhhnklee nsirqpfarl
      781 nprskvaavv avtssntvie rnrfsnprsd yelgshlddh gsiinaslnf wgldtytndv
      841 svriynrifd rkdrynlapv qflqyllngq dldtkeqiss nferdkimqf qtagnkeigg
      901 evmgdvtler gdytvkkdif vrrgskllik pgtrlqfeqs igmmvegrle sdgtseqivy
      961 talsvpmead etetlptemp slrlssvteg tievtvngtt ggvcaygfti eaaalvcqql
     1021 gmvlsshdwl mepasqassg gtshilfsnv rcsemdtdfl tcrhevlged fenscfsevg
     1081 ircirpswag irlgvhaets ilknvvieka gqldyathvf kpalqvdfnn hileklnire
     1141 ntdsgiglmw ndifrdihnp vvkssevynn nrhgiltrsq gvditenriq gnrgggvwyd
     1201 pvftkreqrd avrwaedtat visdnirlnp derhyirvae aspntrrefr vscedkraig
     1261 imvvdpired stetllirrg qdfedtsapm wdirknltsf plretgfivt lryetgsapk
     1321 ggallmmtav qplgqaqmle yklrlniksn iiegcgwgfg snhynrdiap deafyyhrys
     1381 netvhlvknt itesrqhavh vssplwdplt sslaeivynl tenrinnnqg ginhysrdlr
     1441 ssnnlfhwii nrtefsnnng gidlrlpyvw dynenythsm aivnctfknn rnfklridgh
     1501 farfnatwni iednechsge gvftvagmek emlitrntfr rnrgkyvvef kleshserfs
     1561 ivganfyynm lqnnqpdass gvspdhyepn syavsirgvq rinitqnflv npdheyefla
     1621 gvftsslnna vnvaenwwgs lnatdiksrv fdlddwngya aadfspylar gefggptlsy
     1681 dgsdewgfrm dtsrplggrl fksitlyqrs spyiirsdlt ilpnatltil egvelqfyps
     1741 vgilvlgnli avgrpwapir mgplkqlqqs frykraspkr fvrlcvdqac sadrrdgfle
     1801 ffnsttlqwv pvcdqrfter naevvcrelg fgtldvwidr gyrhelgpte lsiinswlhp
     1861 lqctghepsl sscefrlngy gehsyacspf dhkfvyihcg eelttpeeef wggirfsvpd
     1921 ykaqnsmggg vyassryytp ptsalrhvhi rkagilhnek gpavqvvlrd vtlehlnise
     1981 casngidiia spgyqyihav nvthnrgtgi nfvtlngqts taerlnyipl gpvdipynvf
     2041 glvdicdnnk afpvdgkvll yykydnrpve cikiftsksr lsqkvgfril qfnlfnstef
     2101 tpypdsisif dgdvfnstvk kighmsvgtd skestaqttf yrsqgitlsv klhatgasgl
     2161 ngfiaevvti plqtitards khnitasyfn knrrgaityr sageitpilt lhgnrfedng
     2221 dalfgnfsss eaavffdiqn smemhilrnl fiynqgglri fvsstnsvsa lkgyvhnnlf
     2281 tqnknresly vrsgetgmyq ylhvyrnyfa resaafhdnv vfekvtfnfs enmivesegy
     2341 rqmsvlgfek tqssgqlvyr nwlwdneafa kelkatvlae ssgqkfskny flnrlnyfem
     2401 vtannteqen ssyvdaennw wgfketsavv griydrsdqp ellevrfqph leyndtvisg
     2461 ncmggwemie dtcflyipgp mthaeakrfc qldassmpyl kqkltklmqy istwqedfdw
     2521 lyeriwvqsl dvpldqcavl yrggvwahdc ndrlpflcer aqeivvrsdy wyqepvsita
     2581 msllgvllic vciilgfwwc ksqerhkqhl qrqnsirasi rsasnrsldr daffsdykhr
     2641 ieraladtpt stpprlavhp pkhngstdsv akqytydldd srsyddtvhe vrnpaldyss
     2701 dieekyptsv pgtrkgaadl qhptfdltye nrgymdrsvg gyddsldasr dwssgtdstl
     2761 dmkrsletpq avpmdpsdnt fkapepyqps hyantdnyyr rqpletam
//
LOCUS       XP_077502439            1742 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat-rich membrane spanning [Amblyomma americanum].
ACCESSION   XP_077502439
VERSION     XP_077502439.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646313.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1742
                     /product="ankyrin repeat-rich membrane spanning"
                     /calculated_mol_wt=191927
     Region          24..306
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          37..68
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          70..101
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          104..134
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(105,109..110,113..115,117..118,122,125,134,136,138,
                     142..143,146..148,150..151,155,158,167,169,171,175..176,
                     179..181,183..184,188,191)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          136..167
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          169..197
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          185..456
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          202..233
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          235..266
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          268..299
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          301..329
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          334..365
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          440..980
                     /region_name="KAP_NTPase"
                     /note="KAP family P-loop domain; cl46386"
                     /db_xref="CDD:480726"
     CDS             1..1742
                     /gene="Arms"
                     /coded_by="XM_077646313.1:207..5435"
                     /db_xref="GeneID:144113320"
ORIGIN      
        1 masaftsaaa ainagdlhsl erslnskivs pderdengqt llmiaaqcgq lpiveelirq
       61 gadvhledld nwtallnaak egytdvvsel vmhnadiehr dmggwtalmw acykghteta
      121 kylleqgafp nvhdlnhmsc liwaagrghq stvgdllsyg akvnsgdkyg ttplvwacrr
      181 gftgivemll eaganvdtag myswtaliia tkgnysdivs mlleykpnvn avdadgytal
      241 aiackegftd iavsllnaga yvnmhdrsgd tnlihaakag nfsivdvllk kyadvdvqgm
      301 drktalywav ekgytdivrv ilnanpnlel ptkdgdtplm katrirstei vqmlidkkak
      361 vsatdkrgdt alhiamrars kgiveillrn pknsqllyrp nrsnetpyni dtshqksila
      421 qifgarrlnt nednenmlgy dlyssaladi lsepslsmpi svglyarwgs gksfllgkle
      481 edmrsfavqw meptfsfswv lfslvsfysc mlgycvllat gdwllgiilg agsfmltyci
      541 lyiirygssh hdwnwaykfc ikmenmlssf ktvlqvifch ppatnkdfgs npvrfifteq
      601 nkvsssgagd asiayiiisl fsaiedefgf fttrfyhvfq pkpvassswt wrrlccvpnf
      661 aiflltflcl laavavlvet lagngpyvvs sgqtqpllia lasvvavvll tnlytwvrmv
      721 lslfrshhrr ihsylshvda tsggymealk geielmsamv hcldafvgkq trlvlivdgl
      781 dsceqdkvlr vldavyvlfs dpghpfvtil aldphiiika ieanlhrafq nttvrghdyl
      841 rnivhlpfyl qnsglrkvra aqqtavglkr sgshwcekee vekaaqgivs tgpdrpgnrr
      901 vsnssmsipe sfrkpsqqrk gnqklkases vassmsnlhr gvtgaqdltk vlltddyfsd
      961 inprsmrrlm nivyvtgrll kafnidfnwy hlaswvnite qwpyhtswii lyyelhenei
     1021 ddatslrsif ekikgkvptg kdlqplydvd hdvrkfeifl sfhsasllis dlkifvpfti
     1081 nldpyirrvi qeryqsqses eklvhkcgsg pfgppapplg pvgmmgpaga pwtplkaelm
     1141 ypqapptpvm psepyqqqpy hlwwsvgspg asstmpvapl vrvwnepftg rlsaltvegv
     1201 snlleglegi dksrvpdyqg tltrhnihgq vlltcnisel revlamtfgd weifkmails
     1261 lreqeyqraq psaapedvpp vpfsrdrths vsatvpgdht dskakdktnd gseksdkssh
     1321 sqttgrrpns slekqvtmee slivgaletl seeamedtld esvaphagfh hsqvlspils
     1381 dpgegeeasg tagrttgdne vdvvylkmph gspkynyspl sasvpdyewg vqgpsptvyc
     1441 stdlplesyg vsappsiass pnapppgsfd sasfmskwdt agqkprnkhl dsvvaqegms
     1501 rrgslsrdvp essliehwkg lggsdlqqer lrlemlslpn daaedenapl ievtrpmflg
     1561 tlssrdaess srrerefiqp fklargarrf nierqfsvde rsdidpdehk pdggkststl
     1621 sltmlksadg ttetssaata ltplssssgr speeelqvrr nsvpsaqsde qttseggvvp
     1681 sqsapfkppe mssrtflqql aeqdqmgqag qplcvgtssd tvviagkatd akhlfssset
     1741 vi
//
LOCUS       XP_077502440            1742 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat-rich membrane spanning [Amblyomma americanum].
ACCESSION   XP_077502440
VERSION     XP_077502440.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646314.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1742
                     /product="ankyrin repeat-rich membrane spanning"
                     /calculated_mol_wt=191927
     Region          24..306
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          37..68
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          70..101
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          104..134
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(105,109..110,113..115,117..118,122,125,134,136,138,
                     142..143,146..148,150..151,155,158,167,169,171,175..176,
                     179..181,183..184,188,191)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          136..167
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          169..197
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          185..456
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          202..233
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          235..266
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          268..299
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          301..329
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          334..365
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          440..980
                     /region_name="KAP_NTPase"
                     /note="KAP family P-loop domain; cl46386"
                     /db_xref="CDD:480726"
     CDS             1..1742
                     /gene="Arms"
                     /coded_by="XM_077646314.1:157..5385"
                     /db_xref="GeneID:144113320"
ORIGIN      
        1 masaftsaaa ainagdlhsl erslnskivs pderdengqt llmiaaqcgq lpiveelirq
       61 gadvhledld nwtallnaak egytdvvsel vmhnadiehr dmggwtalmw acykghteta
      121 kylleqgafp nvhdlnhmsc liwaagrghq stvgdllsyg akvnsgdkyg ttplvwacrr
      181 gftgivemll eaganvdtag myswtaliia tkgnysdivs mlleykpnvn avdadgytal
      241 aiackegftd iavsllnaga yvnmhdrsgd tnlihaakag nfsivdvllk kyadvdvqgm
      301 drktalywav ekgytdivrv ilnanpnlel ptkdgdtplm katrirstei vqmlidkkak
      361 vsatdkrgdt alhiamrars kgiveillrn pknsqllyrp nrsnetpyni dtshqksila
      421 qifgarrlnt nednenmlgy dlyssaladi lsepslsmpi svglyarwgs gksfllgkle
      481 edmrsfavqw meptfsfswv lfslvsfysc mlgycvllat gdwllgiilg agsfmltyci
      541 lyiirygssh hdwnwaykfc ikmenmlssf ktvlqvifch ppatnkdfgs npvrfifteq
      601 nkvsssgagd asiayiiisl fsaiedefgf fttrfyhvfq pkpvassswt wrrlccvpnf
      661 aiflltflcl laavavlvet lagngpyvvs sgqtqpllia lasvvavvll tnlytwvrmv
      721 lslfrshhrr ihsylshvda tsggymealk geielmsamv hcldafvgkq trlvlivdgl
      781 dsceqdkvlr vldavyvlfs dpghpfvtil aldphiiika ieanlhrafq nttvrghdyl
      841 rnivhlpfyl qnsglrkvra aqqtavglkr sgshwcekee vekaaqgivs tgpdrpgnrr
      901 vsnssmsipe sfrkpsqqrk gnqklkases vassmsnlhr gvtgaqdltk vlltddyfsd
      961 inprsmrrlm nivyvtgrll kafnidfnwy hlaswvnite qwpyhtswii lyyelhenei
     1021 ddatslrsif ekikgkvptg kdlqplydvd hdvrkfeifl sfhsasllis dlkifvpfti
     1081 nldpyirrvi qeryqsqses eklvhkcgsg pfgppapplg pvgmmgpaga pwtplkaelm
     1141 ypqapptpvm psepyqqqpy hlwwsvgspg asstmpvapl vrvwnepftg rlsaltvegv
     1201 snlleglegi dksrvpdyqg tltrhnihgq vlltcnisel revlamtfgd weifkmails
     1261 lreqeyqraq psaapedvpp vpfsrdrths vsatvpgdht dskakdktnd gseksdkssh
     1321 sqttgrrpns slekqvtmee slivgaletl seeamedtld esvaphagfh hsqvlspils
     1381 dpgegeeasg tagrttgdne vdvvylkmph gspkynyspl sasvpdyewg vqgpsptvyc
     1441 stdlplesyg vsappsiass pnapppgsfd sasfmskwdt agqkprnkhl dsvvaqegms
     1501 rrgslsrdvp essliehwkg lggsdlqqer lrlemlslpn daaedenapl ievtrpmflg
     1561 tlssrdaess srrerefiqp fklargarrf nierqfsvde rsdidpdehk pdggkststl
     1621 sltmlksadg ttetssaata ltplssssgr speeelqvrr nsvpsaqsde qttseggvvp
     1681 sqsapfkppe mssrtflqql aeqdqmgqag qplcvgtssd tvviagkatd akhlfssset
     1741 vi
//
LOCUS       XP_077502441            1742 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat-rich membrane spanning [Amblyomma americanum].
ACCESSION   XP_077502441
VERSION     XP_077502441.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646315.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1742
                     /product="ankyrin repeat-rich membrane spanning"
                     /calculated_mol_wt=191927
     Region          24..306
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          37..68
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          70..101
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          104..134
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(105,109..110,113..115,117..118,122,125,134,136,138,
                     142..143,146..148,150..151,155,158,167,169,171,175..176,
                     179..181,183..184,188,191)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          136..167
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          169..197
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          185..456
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          202..233
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          235..266
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          268..299
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          301..329
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          334..365
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          440..980
                     /region_name="KAP_NTPase"
                     /note="KAP family P-loop domain; cl46386"
                     /db_xref="CDD:480726"
     CDS             1..1742
                     /gene="Arms"
                     /coded_by="XM_077646315.1:149..5377"
                     /db_xref="GeneID:144113320"
ORIGIN      
        1 masaftsaaa ainagdlhsl erslnskivs pderdengqt llmiaaqcgq lpiveelirq
       61 gadvhledld nwtallnaak egytdvvsel vmhnadiehr dmggwtalmw acykghteta
      121 kylleqgafp nvhdlnhmsc liwaagrghq stvgdllsyg akvnsgdkyg ttplvwacrr
      181 gftgivemll eaganvdtag myswtaliia tkgnysdivs mlleykpnvn avdadgytal
      241 aiackegftd iavsllnaga yvnmhdrsgd tnlihaakag nfsivdvllk kyadvdvqgm
      301 drktalywav ekgytdivrv ilnanpnlel ptkdgdtplm katrirstei vqmlidkkak
      361 vsatdkrgdt alhiamrars kgiveillrn pknsqllyrp nrsnetpyni dtshqksila
      421 qifgarrlnt nednenmlgy dlyssaladi lsepslsmpi svglyarwgs gksfllgkle
      481 edmrsfavqw meptfsfswv lfslvsfysc mlgycvllat gdwllgiilg agsfmltyci
      541 lyiirygssh hdwnwaykfc ikmenmlssf ktvlqvifch ppatnkdfgs npvrfifteq
      601 nkvsssgagd asiayiiisl fsaiedefgf fttrfyhvfq pkpvassswt wrrlccvpnf
      661 aiflltflcl laavavlvet lagngpyvvs sgqtqpllia lasvvavvll tnlytwvrmv
      721 lslfrshhrr ihsylshvda tsggymealk geielmsamv hcldafvgkq trlvlivdgl
      781 dsceqdkvlr vldavyvlfs dpghpfvtil aldphiiika ieanlhrafq nttvrghdyl
      841 rnivhlpfyl qnsglrkvra aqqtavglkr sgshwcekee vekaaqgivs tgpdrpgnrr
      901 vsnssmsipe sfrkpsqqrk gnqklkases vassmsnlhr gvtgaqdltk vlltddyfsd
      961 inprsmrrlm nivyvtgrll kafnidfnwy hlaswvnite qwpyhtswii lyyelhenei
     1021 ddatslrsif ekikgkvptg kdlqplydvd hdvrkfeifl sfhsasllis dlkifvpfti
     1081 nldpyirrvi qeryqsqses eklvhkcgsg pfgppapplg pvgmmgpaga pwtplkaelm
     1141 ypqapptpvm psepyqqqpy hlwwsvgspg asstmpvapl vrvwnepftg rlsaltvegv
     1201 snlleglegi dksrvpdyqg tltrhnihgq vlltcnisel revlamtfgd weifkmails
     1261 lreqeyqraq psaapedvpp vpfsrdrths vsatvpgdht dskakdktnd gseksdkssh
     1321 sqttgrrpns slekqvtmee slivgaletl seeamedtld esvaphagfh hsqvlspils
     1381 dpgegeeasg tagrttgdne vdvvylkmph gspkynyspl sasvpdyewg vqgpsptvyc
     1441 stdlplesyg vsappsiass pnapppgsfd sasfmskwdt agqkprnkhl dsvvaqegms
     1501 rrgslsrdvp essliehwkg lggsdlqqer lrlemlslpn daaedenapl ievtrpmflg
     1561 tlssrdaess srrerefiqp fklargarrf nierqfsvde rsdidpdehk pdggkststl
     1621 sltmlksadg ttetssaata ltplssssgr speeelqvrr nsvpsaqsde qttseggvvp
     1681 sqsapfkppe mssrtflqql aeqdqmgqag qplcvgtssd tvviagkatd akhlfssset
     1741 vi
//
LOCUS       XP_077502442             432 aa            linear   INV 25-NOV-2025
DEFINITION  misexpression suppressor of KSR 2 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502442
VERSION     XP_077502442.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646316.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..432
                     /product="misexpression suppressor of KSR 2 isoform X1"
                     /calculated_mol_wt=48640
     Region          71..358
                     /region_name="alpha/beta hydrolases"
                     /note="A functionally diverse superfamily containing
                     proteases, lipases, peroxidases, esterases, epoxide
                     hydrolases and dehalogenases. The catalytic apparatus
                     typically involves three residues (catalytic triad): a
                     serine, a glutamate or aspartate and a...; cl21494"
                     /db_xref="CDD:473884"
     CDS             1..432
                     /gene="MESK2"
                     /coded_by="XM_077646316.1:1050..2348"
                     /db_xref="GeneID:144113321"
ORIGIN      
        1 mdaalsklak kdvpyakmip vqkdsdksef veaqdergmp tdtmddielk nvelqyplmr
       61 slsrsdsytq edrietdfgt mvvavqgdrn kpaiftyhdi glnhvsnfqa ffnytdvrdm
      121 mqnfcvyhvn apgqeegaap lpegyaypsm etladmlllv mkfykikhfi gfgvgagayi
      181 lakfaldrpe lvdalflinc tatksswtew gyqklnamhl rssgmtastl dylmwhhfgk
      241 lneernhdlv qvfrqyfnkt inaqnlgffi dsfikrsdln itremdpvkk kmvknfkctv
      301 mlvagalsph lddtvnmnar mdpgtsswmk vsdcgmvlee qpgkvseafr lflqglgyal
      361 nssqrrssaa slnealemrr rssltasahn avpslagqkl ssvrqtslee eanakqevhi
      421 venpinqhqv nc
//
LOCUS       XP_077502443             394 aa            linear   INV 25-NOV-2025
DEFINITION  misexpression suppressor of KSR 2 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502443
VERSION     XP_077502443.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646317.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..394
                     /product="misexpression suppressor of KSR 2 isoform X2"
                     /calculated_mol_wt=44447
     Region          71..358
                     /region_name="Abhydrolase"
                     /note="alpha/beta hydrolases; cl21494"
                     /db_xref="CDD:473884"
     CDS             1..394
                     /gene="MESK2"
                     /coded_by="XM_077646317.1:1036..2220"
                     /db_xref="GeneID:144113321"
ORIGIN      
        1 mdaalsklak kdvpyakmip vqkdsdksef veaqdergmp tdtmddielk nvelqyplmr
       61 slsrsdsytq edrietdfgt mvvavqgdrn kpaiftyhdi glnhvsnfqa ffnytdvrdm
      121 mqnfcvyhvn apgqeegaap lpegyaypsm etladmlllv mkfykikhfi gfgvgagayi
      181 lakfaldrpe lvdalflinc tatksswtew gyqklnamhl rssgmtastl dylmwhhfgk
      241 lneernhdlv qvfrqyfnkt inaqnlgffi dsfikrsdln itremdpvkk kmvknfkctv
      301 mlvagalsph lddtvnmnar mdpgtsswmk vsdcgmvlee qpgkvseafr lflqglgyal
      361 nssqrrssaa slnealemrr rssltasahn adea
//
LOCUS       XP_077502444             394 aa            linear   INV 25-NOV-2025
DEFINITION  misexpression suppressor of KSR 2 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502444
VERSION     XP_077502444.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646318.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..394
                     /product="misexpression suppressor of KSR 2 isoform X3"
                     /calculated_mol_wt=44418
     Region          33..320
                     /region_name="Abhydrolase"
                     /note="alpha/beta hydrolases; cl21494"
                     /db_xref="CDD:473884"
     CDS             1..394
                     /gene="MESK2"
                     /coded_by="XM_077646318.1:210..1394"
                     /db_xref="GeneID:144113321"
ORIGIN      
        1 mptdtmddie lknvelqypl mrslsrsdsy tqedrietdf gtmvvavqgd rnkpaiftyh
       61 diglnhvsnf qaffnytdvr dmmqnfcvyh vnapgqeega aplpegyayp smetladmll
      121 lvmkfykikh figfgvgaga yilakfaldr pelvdalfli nctatksswt ewgyqklnam
      181 hlrssgmtas tldylmwhhf gklneernhd lvqvfrqyfn ktinaqnlgf fidsfikrsd
      241 lnitremdpv kkkmvknfkc tvmlvagals phlddtvnmn armdpgtssw mkvsdcgmvl
      301 eeqpgkvsea frlflqglgy alnssqrrss aaslnealem rrrssltasa hnavpslagq
      361 klssvrqtsl eeeanakqev hivenpinqh qvnc
//
LOCUS       XP_077502445             394 aa            linear   INV 25-NOV-2025
DEFINITION  misexpression suppressor of KSR 2 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502445
VERSION     XP_077502445.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646319.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..394
                     /product="misexpression suppressor of KSR 2 isoform X3"
                     /calculated_mol_wt=44418
     Region          33..320
                     /region_name="Abhydrolase"
                     /note="alpha/beta hydrolases; cl21494"
                     /db_xref="CDD:473884"
     CDS             1..394
                     /gene="MESK2"
                     /coded_by="XM_077646319.1:211..1395"
                     /db_xref="GeneID:144113321"
ORIGIN      
        1 mptdtmddie lknvelqypl mrslsrsdsy tqedrietdf gtmvvavqgd rnkpaiftyh
       61 diglnhvsnf qaffnytdvr dmmqnfcvyh vnapgqeega aplpegyayp smetladmll
      121 lvmkfykikh figfgvgaga yilakfaldr pelvdalfli nctatksswt ewgyqklnam
      181 hlrssgmtas tldylmwhhf gklneernhd lvqvfrqyfn ktinaqnlgf fidsfikrsd
      241 lnitremdpv kkkmvknfkc tvmlvagals phlddtvnmn armdpgtssw mkvsdcgmvl
      301 eeqpgkvsea frlflqglgy alnssqrrss aaslnealem rrrssltasa hnavpslagq
      361 klssvrqtsl eeeanakqev hivenpinqh qvnc
//
LOCUS       XP_077502446             394 aa            linear   INV 25-NOV-2025
DEFINITION  misexpression suppressor of KSR 2 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502446
VERSION     XP_077502446.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646320.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..394
                     /product="misexpression suppressor of KSR 2 isoform X3"
                     /calculated_mol_wt=44418
     Region          33..320
                     /region_name="Abhydrolase"
                     /note="alpha/beta hydrolases; cl21494"
                     /db_xref="CDD:473884"
     CDS             1..394
                     /gene="MESK2"
                     /coded_by="XM_077646320.1:156..1340"
                     /db_xref="GeneID:144113321"
ORIGIN      
        1 mptdtmddie lknvelqypl mrslsrsdsy tqedrietdf gtmvvavqgd rnkpaiftyh
       61 diglnhvsnf qaffnytdvr dmmqnfcvyh vnapgqeega aplpegyayp smetladmll
      121 lvmkfykikh figfgvgaga yilakfaldr pelvdalfli nctatksswt ewgyqklnam
      181 hlrssgmtas tldylmwhhf gklneernhd lvqvfrqyfn ktinaqnlgf fidsfikrsd
      241 lnitremdpv kkkmvknfkc tvmlvagals phlddtvnmn armdpgtssw mkvsdcgmvl
      301 eeqpgkvsea frlflqglgy alnssqrrss aaslnealem rrrssltasa hnavpslagq
      361 klssvrqtsl eeeanakqev hivenpinqh qvnc
//
LOCUS       XP_077502447             356 aa            linear   INV 25-NOV-2025
DEFINITION  misexpression suppressor of KSR 2 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077502447
VERSION     XP_077502447.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646321.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..356
                     /product="misexpression suppressor of KSR 2 isoform X4"
                     /calculated_mol_wt=40225
     Region          33..320
                     /region_name="Abhydrolase"
                     /note="alpha/beta hydrolases; cl21494"
                     /db_xref="CDD:473884"
     CDS             1..356
                     /gene="MESK2"
                     /coded_by="XM_077646321.1:148..1218"
                     /db_xref="GeneID:144113321"
ORIGIN      
        1 mptdtmddie lknvelqypl mrslsrsdsy tqedrietdf gtmvvavqgd rnkpaiftyh
       61 diglnhvsnf qaffnytdvr dmmqnfcvyh vnapgqeega aplpegyayp smetladmll
      121 lvmkfykikh figfgvgaga yilakfaldr pelvdalfli nctatksswt ewgyqklnam
      181 hlrssgmtas tldylmwhhf gklneernhd lvqvfrqyfn ktinaqnlgf fidsfikrsd
      241 lnitremdpv kkkmvknfkc tvmlvagals phlddtvnmn armdpgtssw mkvsdcgmvl
      301 eeqpgkvsea frlflqglgy alnssqrrss aaslnealem rrrssltasa hnadea
//
LOCUS       XP_077502448             356 aa            linear   INV 25-NOV-2025
DEFINITION  misexpression suppressor of KSR 2 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077502448
VERSION     XP_077502448.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646322.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..356
                     /product="misexpression suppressor of KSR 2 isoform X4"
                     /calculated_mol_wt=40225
     Region          33..320
                     /region_name="Abhydrolase"
                     /note="alpha/beta hydrolases; cl21494"
                     /db_xref="CDD:473884"
     CDS             1..356
                     /gene="MESK2"
                     /coded_by="XM_077646322.1:171..1241"
                     /db_xref="GeneID:144113321"
ORIGIN      
        1 mptdtmddie lknvelqypl mrslsrsdsy tqedrietdf gtmvvavqgd rnkpaiftyh
       61 diglnhvsnf qaffnytdvr dmmqnfcvyh vnapgqeega aplpegyayp smetladmll
      121 lvmkfykikh figfgvgaga yilakfaldr pelvdalfli nctatksswt ewgyqklnam
      181 hlrssgmtas tldylmwhhf gklneernhd lvqvfrqyfn ktinaqnlgf fidsfikrsd
      241 lnitremdpv kkkmvknfkc tvmlvagals phlddtvnmn armdpgtssw mkvsdcgmvl
      301 eeqpgkvsea frlflqglgy alnssqrrss aaslnealem rrrssltasa hnadea
//
LOCUS       XP_077502449             356 aa            linear   INV 25-NOV-2025
DEFINITION  misexpression suppressor of KSR 2 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077502449
VERSION     XP_077502449.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646323.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..356
                     /product="misexpression suppressor of KSR 2 isoform X4"
                     /calculated_mol_wt=40225
     Region          33..320
                     /region_name="Abhydrolase"
                     /note="alpha/beta hydrolases; cl21494"
                     /db_xref="CDD:473884"
     CDS             1..356
                     /gene="MESK2"
                     /coded_by="XM_077646323.1:146..1216"
                     /db_xref="GeneID:144113321"
ORIGIN      
        1 mptdtmddie lknvelqypl mrslsrsdsy tqedrietdf gtmvvavqgd rnkpaiftyh
       61 diglnhvsnf qaffnytdvr dmmqnfcvyh vnapgqeega aplpegyayp smetladmll
      121 lvmkfykikh figfgvgaga yilakfaldr pelvdalfli nctatksswt ewgyqklnam
      181 hlrssgmtas tldylmwhhf gklneernhd lvqvfrqyfn ktinaqnlgf fidsfikrsd
      241 lnitremdpv kkkmvknfkc tvmlvagals phlddtvnmn armdpgtssw mkvsdcgmvl
      301 eeqpgkvsea frlflqglgy alnssqrrss aaslnealem rrrssltasa hnadea
//
LOCUS       XP_077502451             427 aa            linear   INV 25-NOV-2025
DEFINITION  sodium-dependent organic anion transporter-like [Amblyomma
            americanum].
ACCESSION   XP_077502451
VERSION     XP_077502451.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646325.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..427
                     /product="sodium-dependent organic anion transporter-like"
                     /calculated_mol_wt=46281
     Region          131..410
                     /region_name="SBF"
                     /note="Sodium Bile acid symporter family; cl19217"
                     /db_xref="CDD:450274"
     CDS             1..427
                     /gene="LOC144113323"
                     /coded_by="XM_077646325.1:927..2210"
                     /db_xref="GeneID:144113323"
ORIGIN      
        1 mwllhsclvv tvlltqssqc vlnfssqesr lqlyepvnvt fcftqqlska nlvlrssdss
       61 vvqlnedevt mslervapvd nfstlltplk igyadllwqv edgltngtyn gihkmcvvre
      121 ltafsmgfga vigvlvalnn vnvgcqldmr vvlsqvkrpi apaigmfcqf ifmplvsygi
      181 amlllqnavt rlglftigsc pggiysnfyt lllqgdldls vtmtfmssvg alalmpmwmh
      241 tlgrqiagsa apavpfsnlm vslvaltlpl glglliqykc pkwaqtseki vkpfsvaill
      301 valvigtysy wyivtlitlm dvvaafvvvv aaflfgatma wlfrlnhkqi iavsletaiq
      361 ngplaivvlt fslpepsgdl avvpvvvlvt lssailltlf ailtvrqkcs tyaracqddv
      421 kletsvn
//
LOCUS       XP_077502452             138 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113324 [Amblyomma americanum].
ACCESSION   XP_077502452
VERSION     XP_077502452.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646326.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..138
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..138
                     /product="uncharacterized protein LOC144113324"
                     /calculated_mol_wt=15253
     Region          63..>120
                     /region_name="BEN"
                     /note="The BEN domain is found in diverse animal proteins;
                     smart01025"
                     /db_xref="CDD:214981"
     CDS             1..138
                     /gene="LOC144113324"
                     /coded_by="XM_077646326.1:407..823"
                     /db_xref="GeneID:144113324"
ORIGIN      
        1 msflflllav pakmvkrler mleskkepdq pvvpcakvdi gngvlvdrti lqmlerqcqs
       61 spgkfarall rnvfteeelr gkslygmknn vqkglpvkea ldpvrlsavi gytcdhfpgv
      121 nvaylknsla allarelk
//
LOCUS       XP_077502453             805 aa            linear   INV 25-NOV-2025
DEFINITION  mdm2-binding protein-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502453
VERSION     XP_077502453.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646327.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..805
                     /product="mdm2-binding protein-like isoform X1"
                     /calculated_mol_wt=89913
     Region          541..787
                     /region_name="MTBP_C"
                     /note="MDM2-binding; pfam14920"
                     /db_xref="CDD:464376"
     CDS             1..805
                     /gene="LOC144113325"
                     /coded_by="XM_077646327.1:478..2895"
                     /db_xref="GeneID:144113325"
ORIGIN      
        1 mkwnclflir diagkdivee ylhrlrvlta stswkvhsva shllplmpdr nihfailpfl
       61 lstceetevs wmsshlglfe salqswledi eklqeiyhfp pelspaegds aakrindlld
      121 rlpchqaldi vvdqrifsdl eirsnvslyg allrahvwhc atfrllgsgf nsaqidswld
      181 hldicdvelr tdwmpmwkga inwgeglplc hfelqcsvpp peltalhsta eafvdilase
      241 dnlrwslhle vleefdfnsl pmhlviplpf qlvstaspqs atamflehci ankkrcvalr
      301 lsfwdnveik tqrtrlttse wrrcllgegt lhpsflndpc lqepnghlhf vlvsngssaf
      361 lfplrktppv lgtclysnre eshiptnped stielladip lvssssilks vhrantlrla
      421 tlrnflangq ssshdieele kslskltvde sdwisdsaed lsmslsdhdt iascgdpsdw
      481 perrflvsns lkglasrenc smpkgvisis ageilklfde sgrarrkplh rvhvlgqntk
      541 psvteeqvls mqwpdvllck yhdlyyntns ealesaysaa rekfvsdeta ttcsssvess
      601 vvplskvrkl sskapvrksp rklpvsrsht tlwdqkqkrf lastrsarva saphrrsprk
      661 aaatqnvlqk tvplvqsnkr vaaspillrs sprkndrklq ldetsrkevc dpvctvpsaa
      721 eklrlklrva vanalekngv tqssllykpc gkklfaictt faqdlvgtgr tsellqkiad
      781 shakqvvkfe knkaghrksg tsaas
//
LOCUS       XP_077502454             804 aa            linear   INV 25-NOV-2025
DEFINITION  mdm2-binding protein-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502454
VERSION     XP_077502454.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646328.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..804
                     /product="mdm2-binding protein-like isoform X2"
                     /calculated_mol_wt=89826
     Region          541..786
                     /region_name="MTBP_C"
                     /note="MDM2-binding; pfam14920"
                     /db_xref="CDD:464376"
     CDS             1..804
                     /gene="LOC144113325"
                     /coded_by="XM_077646328.1:460..2874"
                     /db_xref="GeneID:144113325"
ORIGIN      
        1 mkwnclflir diagkdivee ylhrlrvlta stswkvhsva shllplmpdr nihfailpfl
       61 lstceetevs wmsshlglfe salqswledi eklqeiyhfp pelspaegds aakrindlld
      121 rlpchqaldi vvdqrifsdl eirsnvslyg allrahvwhc atfrllgsgf nsaqidswld
      181 hldicdvelr tdwmpmwkga inwgeglplc hfelqcsvpp peltalhsta eafvdilase
      241 dnlrwslhle vleefdfnsl pmhlviplpf qlvstaspqs atamflehci ankkrcvalr
      301 lsfwdnveik tqrtrlttse wrrcllgegt lhpsflndpc lqepnghlhf vlvsngssaf
      361 lfplrktppv lgtclysnre eshiptnped stielladip lvssssilks vhrantlrla
      421 tlrnflangq ssshdieele kslskltvde sdwisdsaed lsmslsdhdt iascgdpsdw
      481 perrflvsns lkglasrenc smpkgvisis ageilklfde sgrarrkplh rvhvlgqntk
      541 psvteeqvls mqwpdvllck yhdlyyntns ealesaysaa rekfvsdeta ttcsssvess
      601 vvplskvrkl sskapvrksp rklpvsrsht tlwdqkqkrf lastrsarva saphrrsprk
      661 aaatqnvlqk tvplvqsnkr vaaspillrs sprkndrklq ldetrkevcd pvctvpsaae
      721 klrlklrvav analekngvt qssllykpcg kklfaicttf aqdlvgtgrt sellqkiads
      781 hakqvvkfek nkaghrksgt saas
//
LOCUS       XP_077502455             276 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein F13E9.13, mitochondrial [Amblyomma
            americanum].
ACCESSION   XP_077502455
VERSION     XP_077502455.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646329.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..276
                     /product="uncharacterized protein F13E9.13, mitochondrial"
                     /calculated_mol_wt=29526
     Region          6..273
                     /region_name="BtpA"
                     /note="Membrane biogenesis protein, BtpA/SgcQ family [Cell
                     wall/membrane/envelope biogenesis]; COG0434"
                     /db_xref="CDD:440203"
     CDS             1..276
                     /gene="LOC144113326"
                     /coded_by="XM_077646329.1:54..884"
                     /db_xref="GeneID:144113326"
ORIGIN      
        1 mvsllkfrem fqsvtcavig mvhvkalpga pfnemkcsqi vdaacreaev ykkcgvdgvl
       61 venmfdlpyv kpcqagpett afmtrictev kgllpsvpcg vqilagnnks alavaaaagl
      121 qfvraegfvf ghvadegfme scagellryr riinaedilv ftdikkkhss haitsdisil
      181 etmkaaeffq sdgvvltgga tgqppsasel qelkaeatvp vllgsgials niedfftada
      241 vivgssfkhg nmwsgmlsds qvrafmekvh llrqnv
//
LOCUS       XP_077502456             245 aa            linear   INV 25-NOV-2025
DEFINITION  eukaryotic translation initiation factor 6 [Amblyomma americanum].
ACCESSION   XP_077502456
VERSION     XP_077502456.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646330.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..245
                     /product="eukaryotic translation initiation factor 6"
                     /calculated_mol_wt=26180
     Region          1..244
                     /region_name="PTZ00136"
                     /note="eukaryotic translation initiation factor 6-like
                     protein; Provisional"
                     /db_xref="CDD:185471"
     Site            order(2..4,203..207)
                     /site_type="other"
                     /note="'Velcro' closure"
                     /db_xref="CDD:238292"
     CDS             1..245
                     /gene="eIF6"
                     /coded_by="XM_077646330.1:119..856"
                     /db_xref="GeneID:144113327"
ORIGIN      
        1 mavraqfenn neigvfaklt nsyclvaigg senfysifeg eladtipvih asiagcriig
       61 rltvgnrhgl lvpntttdqe lqhlrnslpd svkiqrieer lsalgnviac ndyvalthpd
      121 ldketeeiia dvlkvevfrq tiagnvlvgs ycalsnqggl vhprtsiedq gelssllqvp
      181 lvagtvnrgs dvvgagmvvn dwcafcgmdt tstelsvves vfrlnegtpa aitttmkasl
      241 iesmt
//
LOCUS       XP_077502457             217 aa            linear   INV 25-NOV-2025
DEFINITION  tumor protein p53 inducible nuclear protein [Amblyomma americanum].
ACCESSION   XP_077502457
VERSION     XP_077502457.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646331.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="tumor protein p53 inducible nuclear protein"
                     /calculated_mol_wt=23725
     Region          28..214
                     /region_name="DOR"
                     /note="DOR family; pfam14839"
                     /db_xref="CDD:464343"
     CDS             1..217
                     /gene="TP53INP"
                     /coded_by="XM_077646331.1:182..835"
                     /db_xref="GeneID:144113328"
ORIGIN      
        1 mlsgiasyfl napptptaed dvnyqtmeae ddwllvdvtd kdrpqpevps eqphslptal
       61 hvavvdssqe atppsspclm dgswyvtppp cfvarrpvhl etsplenlli ehpsmsvygp
      121 prlrgapltp ppspppsaal pagraqpgpr paalaaragw leqlrpsqrt qvqreqrqla
      181 rsqlerqnrt vqagnrhpkr rdraaarrsg annnrkc
//
LOCUS       XP_077502458             217 aa            linear   INV 25-NOV-2025
DEFINITION  tumor protein p53 inducible nuclear protein [Amblyomma americanum].
ACCESSION   XP_077502458
VERSION     XP_077502458.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646332.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="tumor protein p53 inducible nuclear protein"
                     /calculated_mol_wt=23725
     Region          28..214
                     /region_name="DOR"
                     /note="DOR family; pfam14839"
                     /db_xref="CDD:464343"
     CDS             1..217
                     /gene="TP53INP"
                     /coded_by="XM_077646332.1:442..1095"
                     /db_xref="GeneID:144113328"
ORIGIN      
        1 mlsgiasyfl napptptaed dvnyqtmeae ddwllvdvtd kdrpqpevps eqphslptal
       61 hvavvdssqe atppsspclm dgswyvtppp cfvarrpvhl etsplenlli ehpsmsvygp
      121 prlrgapltp ppspppsaal pagraqpgpr paalaaragw leqlrpsqrt qvqreqrqla
      181 rsqlerqnrt vqagnrhpkr rdraaarrsg annnrkc
//
LOCUS       XP_077502459             217 aa            linear   INV 25-NOV-2025
DEFINITION  tumor protein p53 inducible nuclear protein [Amblyomma americanum].
ACCESSION   XP_077502459
VERSION     XP_077502459.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646333.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="tumor protein p53 inducible nuclear protein"
                     /calculated_mol_wt=23725
     Region          28..214
                     /region_name="DOR"
                     /note="DOR family; pfam14839"
                     /db_xref="CDD:464343"
     CDS             1..217
                     /gene="TP53INP"
                     /coded_by="XM_077646333.1:154..807"
                     /db_xref="GeneID:144113328"
ORIGIN      
        1 mlsgiasyfl napptptaed dvnyqtmeae ddwllvdvtd kdrpqpevps eqphslptal
       61 hvavvdssqe atppsspclm dgswyvtppp cfvarrpvhl etsplenlli ehpsmsvygp
      121 prlrgapltp ppspppsaal pagraqpgpr paalaaragw leqlrpsqrt qvqreqrqla
      181 rsqlerqnrt vqagnrhpkr rdraaarrsg annnrkc
//
LOCUS       XP_077502460             217 aa            linear   INV 25-NOV-2025
DEFINITION  tumor protein p53 inducible nuclear protein [Amblyomma americanum].
ACCESSION   XP_077502460
VERSION     XP_077502460.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646334.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="tumor protein p53 inducible nuclear protein"
                     /calculated_mol_wt=23725
     Region          28..214
                     /region_name="DOR"
                     /note="DOR family; pfam14839"
                     /db_xref="CDD:464343"
     CDS             1..217
                     /gene="TP53INP"
                     /coded_by="XM_077646334.1:69..722"
                     /db_xref="GeneID:144113328"
ORIGIN      
        1 mlsgiasyfl napptptaed dvnyqtmeae ddwllvdvtd kdrpqpevps eqphslptal
       61 hvavvdssqe atppsspclm dgswyvtppp cfvarrpvhl etsplenlli ehpsmsvygp
      121 prlrgapltp ppspppsaal pagraqpgpr paalaaragw leqlrpsqrt qvqreqrqla
      181 rsqlerqnrt vqagnrhpkr rdraaarrsg annnrkc
//
LOCUS       XP_077502461             181 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial ribosomal protein L13 [Amblyomma americanum].
ACCESSION   XP_077502461
VERSION     XP_077502461.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646335.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="mitochondrial ribosomal protein L13"
                     /calculated_mol_wt=21565
     Region          18..136
                     /region_name="Ribosomal_L13"
                     /note="Ribosomal protein L13; pfam00572"
                     /db_xref="CDD:459856"
     CDS             1..181
                     /gene="mRpL13"
                     /coded_by="XM_077646335.1:142..687"
                     /db_xref="GeneID:144113330"
ORIGIN      
        1 mgalsnfkrv qqwatftrrw llydarwqnp fqsaemisdv lqgrhkpiff ptndcgdhvv
       61 vvnckeiamp wyewkyrmyy hdtrfaggrs wtpawqvqdk datkvlwkav yralpgdlrr
      121 rplmarlhlf pddevphdil qnisgqlrql revpkklaey seeelsqfpk ifdypddyvi
      181 r
//
LOCUS       XP_077502463             174 aa            linear   INV 25-NOV-2025
DEFINITION  cilia- and flagella-associated protein 418-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077502463
VERSION     XP_077502463.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646337.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..174
                     /product="cilia- and flagella-associated protein 418-like
                     isoform X1"
                     /calculated_mol_wt=19438
     Region          34..174
                     /region_name="RMP"
                     /note="Retinal Maintenance; pfam14996"
                     /db_xref="CDD:464431"
     CDS             1..174
                     /gene="LOC144113331"
                     /coded_by="XM_077646337.1:284..808"
                     /db_xref="GeneID:144113331"
ORIGIN      
        1 mcmeddidil lddveakfck rtgqtmstsa vgassddsie haineicrap elsvpnsvpa
       61 enkllsrkta qcfvvylagt dvvfgvstsa skracshihc crcdfqvaif edfawdqstd
      121 ylffrnsapd lnklrsklvw klgwrayccq cqwrsvaalt saqqeklpwi cvrh
//
LOCUS       XP_077502464             174 aa            linear   INV 25-NOV-2025
DEFINITION  cilia- and flagella-associated protein 418-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077502464
VERSION     XP_077502464.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646338.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..174
                     /product="cilia- and flagella-associated protein 418-like
                     isoform X1"
                     /calculated_mol_wt=19438
     Region          34..174
                     /region_name="RMP"
                     /note="Retinal Maintenance; pfam14996"
                     /db_xref="CDD:464431"
     CDS             1..174
                     /gene="LOC144113331"
                     /coded_by="XM_077646338.1:285..809"
                     /db_xref="GeneID:144113331"
ORIGIN      
        1 mcmeddidil lddveakfck rtgqtmstsa vgassddsie haineicrap elsvpnsvpa
       61 enkllsrkta qcfvvylagt dvvfgvstsa skracshihc crcdfqvaif edfawdqstd
      121 ylffrnsapd lnklrsklvw klgwrayccq cqwrsvaalt saqqeklpwi cvrh
//
LOCUS       XP_077502465             167 aa            linear   INV 25-NOV-2025
DEFINITION  cilia- and flagella-associated protein 418-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077502465
VERSION     XP_077502465.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646339.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..167
                     /product="cilia- and flagella-associated protein 418-like
                     isoform X2"
                     /calculated_mol_wt=18363
     CDS             1..167
                     /gene="LOC144113331"
                     /coded_by="XM_077646339.1:621..1124"
                     /db_xref="GeneID:144113331"
ORIGIN      
        1 mcmeddidil lddveakfck rtgqtmstsa vgassddsie haineicrap elsvpnsvpa
       61 enkllsrkta qcfvvylagt dvvfgvstsa skracshihc crlaslllpv pvafrgrtyv
      121 ctareapldm csalvntsti phmkardlkr cprprtvlrc wrrhrqk
//
LOCUS       XP_077502466             153 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113332 [Amblyomma americanum].
ACCESSION   XP_077502466
VERSION     XP_077502466.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646340.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..153
                     /product="uncharacterized protein LOC144113332"
                     /calculated_mol_wt=16043
     CDS             1..153
                     /gene="LOC144113332"
                     /coded_by="XM_077646340.1:430..891"
                     /db_xref="GeneID:144113332"
ORIGIN      
        1 mahldklstk eledilcqlc tdenikvavs gdddskknla ksagsaiggk vlnklgpfgs
       61 mfgggskdsk dsgakekeae mrpaadvire lpekekdnla dtiqstiskf gdfkdpqall
      121 klitenamvk qlitsavknf lgnflksagg gaa
//
LOCUS       XP_077502467             139 aa            linear   INV 25-NOV-2025
DEFINITION  protein C19orf12 homolog [Amblyomma americanum].
ACCESSION   XP_077502467
VERSION     XP_077502467.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646341.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..139
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..139
                     /product="protein C19orf12 homolog"
                     /calculated_mol_wt=14842
     CDS             1..139
                     /gene="LOC144113333"
                     /coded_by="XM_077646341.1:308..727"
                     /db_xref="GeneID:144113333"
ORIGIN      
        1 mpvrrdemld vlcmisneek lrvtlkhsar gglitgasam aaavimgpvg lavggalggc
       61 vaavlmrdkf vsateiisnm sdhqkdelva avqrafadie vadavelval lcgdihikkr
      121 iiraivdyfh tqmrleiid
//
LOCUS       XP_077502468             139 aa            linear   INV 25-NOV-2025
DEFINITION  protein C19orf12 homolog [Amblyomma americanum].
ACCESSION   XP_077502468
VERSION     XP_077502468.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646342.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..139
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..139
                     /product="protein C19orf12 homolog"
                     /calculated_mol_wt=14842
     CDS             1..139
                     /gene="LOC144113333"
                     /coded_by="XM_077646342.1:250..669"
                     /db_xref="GeneID:144113333"
ORIGIN      
        1 mpvrrdemld vlcmisneek lrvtlkhsar gglitgasam aaavimgpvg lavggalggc
       61 vaavlmrdkf vsateiisnm sdhqkdelva avqrafadie vadavelval lcgdihikkr
      121 iiraivdyfh tqmrleiid
//
LOCUS       XP_077502469            1297 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113334 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502469
VERSION     XP_077502469.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646343.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1297
                     /product="uncharacterized protein LOC144113334 isoform X1"
                     /calculated_mol_wt=145577
     Region          453..473
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(453,456,469,473)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(458,460,462,464..465,468..469,472,486,492..493,
                     496..497,500,513,515,517,519..520,523..524,527)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          479..503
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          481..501
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          508..528
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          535..553
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          582..602
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(582,585,598,602)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          613..633
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          830..850
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(830,833,846,850)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(835,837,839,841..842,845..846,849,863,865,869..870,
                     873..874,878,891,893,895,897..898,901..902,905)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          858..879
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          886..906
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          915..936
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          963..984
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(963,966,980,984)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(968,970,972,974,976,979..980,983,997,999,1003..1004,
                     1007..1008,1011,1025,1027,1029,1031..1032,1035..1036,1039)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          976..1001
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          992..1012
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1020..1040
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1048..1068
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1076..1092
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1124..1143
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1297
                     /gene="LOC144113334"
                     /coded_by="XM_077646343.1:189..4082"
                     /db_xref="GeneID:144113334"
ORIGIN      
        1 mdvficgvch mgfhdigkfv ehknslgsvs cghctaifht qdeldqhalq ehptetqgla
       61 pkeedmlleq svevdpssee gdnnitaaid sattadsqti vvtegfsfea tensvvsdif
      121 acslcqcfal seeemvnhvn echqcnvskd seeapssyyr lqqlaptvvt tpgedkrtlr
      181 mlpvdveepp tqpttlhlvv etssppappk rrrgrprkmd qlqrpvpavv kpeeepdapl
      241 rgqdglfhcs rckrafcker hilghrcmak gdyvdsydkv gvdqkdeeng lvpdeeakda
      301 dttedfrlhr tserepwary rvrrggfrgg rrggisrgtp gspgpessag pgeankenlv
      361 gqdqaaadka aeggaeeaee lpprklhwre dprhvpvfsr deerlafeeh lnrvdlscvd
      421 hlytqhqvaq eitcppgtrp apkevcdlhv fscnvckkvf ktqshmrlhc lihtdlkpft
      481 chqcdfstna kgnlythmrk htgnyfkcsl cdfrscnrsh maeheathst vrqrcelcsn
      541 dyntikslvn hvrkyhtspa gkrylasfqs kqlqnmavlh ichlcnrkfk kkvdrdrhlf
      601 vhnvpeppta yqcglcgyqa srktyldnhy rkhrlvyvcs lcyglhlsat alrahlqkeh
      661 lekqaaeneg elksessmec fvaengtvst aeetvseglq ttseqsdsse ptqttvlsei
      721 ateseataea alsndatles lfadsigqsw ylpepdgitn syanipqelt egsqennsds
      781 psdsqassaq psgplsssva gmwslsqlhy kqlsmevysk irevfgeeec pdcgrlfhsr
      841 idldphrlth tedkpfkcvk cpyssgskdn lkrhqetahe grtfpcercd fvaqsrsslf
      901 qhrqkhtang gqgrcpvcqr qfpswrilrq hmlsqhpdaq qlfkvpggpp rvmgrlgrrt
      961 ykcpycgrvf hrsstdlqkh mwihegvkpf rcpdcpyqcr srnnlnvhrl thsndkphlc
     1021 dqcgkgyksk aalrlhtrth grgvlypcnk ceytatqkch lkrhlethdi irryicehcs
     1081 yssntinymk vhysrkhrge vfnqnsviad pssieqqkdr vykclscsyi fgnmndmkrh
     1141 lrlkhgvcle nldlsveevs aealaseaea aaasaqqemt mepalcedie alevpvgaav
     1201 eeeaaeegtv sliqqmveqg tlaagqevtl qaldaegllt tlnpeaiivr eqdgqplllg
     1261 tqgsylihyv qqdpdgmvlq qgpheqvqqe iiveltp
//
LOCUS       XP_077502470            1297 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113334 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502470
VERSION     XP_077502470.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646344.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1297
                     /product="uncharacterized protein LOC144113334 isoform X1"
                     /calculated_mol_wt=145577
     Region          453..473
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(453,456,469,473)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(458,460,462,464..465,468..469,472,486,492..493,
                     496..497,500,513,515,517,519..520,523..524,527)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          479..503
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          481..501
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          508..528
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          535..553
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          582..602
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(582,585,598,602)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          613..633
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          830..850
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(830,833,846,850)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(835,837,839,841..842,845..846,849,863,865,869..870,
                     873..874,878,891,893,895,897..898,901..902,905)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          858..879
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          886..906
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          915..936
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          963..984
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(963,966,980,984)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(968,970,972,974,976,979..980,983,997,999,1003..1004,
                     1007..1008,1011,1025,1027,1029,1031..1032,1035..1036,1039)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          976..1001
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          992..1012
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1020..1040
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1048..1068
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1076..1092
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1124..1143
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1297
                     /gene="LOC144113334"
                     /coded_by="XM_077646344.1:204..4097"
                     /db_xref="GeneID:144113334"
ORIGIN      
        1 mdvficgvch mgfhdigkfv ehknslgsvs cghctaifht qdeldqhalq ehptetqgla
       61 pkeedmlleq svevdpssee gdnnitaaid sattadsqti vvtegfsfea tensvvsdif
      121 acslcqcfal seeemvnhvn echqcnvskd seeapssyyr lqqlaptvvt tpgedkrtlr
      181 mlpvdveepp tqpttlhlvv etssppappk rrrgrprkmd qlqrpvpavv kpeeepdapl
      241 rgqdglfhcs rckrafcker hilghrcmak gdyvdsydkv gvdqkdeeng lvpdeeakda
      301 dttedfrlhr tserepwary rvrrggfrgg rrggisrgtp gspgpessag pgeankenlv
      361 gqdqaaadka aeggaeeaee lpprklhwre dprhvpvfsr deerlafeeh lnrvdlscvd
      421 hlytqhqvaq eitcppgtrp apkevcdlhv fscnvckkvf ktqshmrlhc lihtdlkpft
      481 chqcdfstna kgnlythmrk htgnyfkcsl cdfrscnrsh maeheathst vrqrcelcsn
      541 dyntikslvn hvrkyhtspa gkrylasfqs kqlqnmavlh ichlcnrkfk kkvdrdrhlf
      601 vhnvpeppta yqcglcgyqa srktyldnhy rkhrlvyvcs lcyglhlsat alrahlqkeh
      661 lekqaaeneg elksessmec fvaengtvst aeetvseglq ttseqsdsse ptqttvlsei
      721 ateseataea alsndatles lfadsigqsw ylpepdgitn syanipqelt egsqennsds
      781 psdsqassaq psgplsssva gmwslsqlhy kqlsmevysk irevfgeeec pdcgrlfhsr
      841 idldphrlth tedkpfkcvk cpyssgskdn lkrhqetahe grtfpcercd fvaqsrsslf
      901 qhrqkhtang gqgrcpvcqr qfpswrilrq hmlsqhpdaq qlfkvpggpp rvmgrlgrrt
      961 ykcpycgrvf hrsstdlqkh mwihegvkpf rcpdcpyqcr srnnlnvhrl thsndkphlc
     1021 dqcgkgyksk aalrlhtrth grgvlypcnk ceytatqkch lkrhlethdi irryicehcs
     1081 yssntinymk vhysrkhrge vfnqnsviad pssieqqkdr vykclscsyi fgnmndmkrh
     1141 lrlkhgvcle nldlsveevs aealaseaea aaasaqqemt mepalcedie alevpvgaav
     1201 eeeaaeegtv sliqqmveqg tlaagqevtl qaldaegllt tlnpeaiivr eqdgqplllg
     1261 tqgsylihyv qqdpdgmvlq qgpheqvqqe iiveltp
//
LOCUS       XP_077502471            1297 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113334 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502471
VERSION     XP_077502471.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646345.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1297
                     /product="uncharacterized protein LOC144113334 isoform X1"
                     /calculated_mol_wt=145577
     Region          453..473
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(453,456,469,473)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(458,460,462,464..465,468..469,472,486,492..493,
                     496..497,500,513,515,517,519..520,523..524,527)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          479..503
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          481..501
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          508..528
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          535..553
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          582..602
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(582,585,598,602)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          613..633
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          830..850
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(830,833,846,850)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(835,837,839,841..842,845..846,849,863,865,869..870,
                     873..874,878,891,893,895,897..898,901..902,905)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          858..879
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          886..906
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          915..936
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          963..984
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(963,966,980,984)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(968,970,972,974,976,979..980,983,997,999,1003..1004,
                     1007..1008,1011,1025,1027,1029,1031..1032,1035..1036,1039)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          976..1001
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          992..1012
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1020..1040
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1048..1068
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1076..1092
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1124..1143
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1297
                     /gene="LOC144113334"
                     /coded_by="XM_077646345.1:183..4076"
                     /db_xref="GeneID:144113334"
ORIGIN      
        1 mdvficgvch mgfhdigkfv ehknslgsvs cghctaifht qdeldqhalq ehptetqgla
       61 pkeedmlleq svevdpssee gdnnitaaid sattadsqti vvtegfsfea tensvvsdif
      121 acslcqcfal seeemvnhvn echqcnvskd seeapssyyr lqqlaptvvt tpgedkrtlr
      181 mlpvdveepp tqpttlhlvv etssppappk rrrgrprkmd qlqrpvpavv kpeeepdapl
      241 rgqdglfhcs rckrafcker hilghrcmak gdyvdsydkv gvdqkdeeng lvpdeeakda
      301 dttedfrlhr tserepwary rvrrggfrgg rrggisrgtp gspgpessag pgeankenlv
      361 gqdqaaadka aeggaeeaee lpprklhwre dprhvpvfsr deerlafeeh lnrvdlscvd
      421 hlytqhqvaq eitcppgtrp apkevcdlhv fscnvckkvf ktqshmrlhc lihtdlkpft
      481 chqcdfstna kgnlythmrk htgnyfkcsl cdfrscnrsh maeheathst vrqrcelcsn
      541 dyntikslvn hvrkyhtspa gkrylasfqs kqlqnmavlh ichlcnrkfk kkvdrdrhlf
      601 vhnvpeppta yqcglcgyqa srktyldnhy rkhrlvyvcs lcyglhlsat alrahlqkeh
      661 lekqaaeneg elksessmec fvaengtvst aeetvseglq ttseqsdsse ptqttvlsei
      721 ateseataea alsndatles lfadsigqsw ylpepdgitn syanipqelt egsqennsds
      781 psdsqassaq psgplsssva gmwslsqlhy kqlsmevysk irevfgeeec pdcgrlfhsr
      841 idldphrlth tedkpfkcvk cpyssgskdn lkrhqetahe grtfpcercd fvaqsrsslf
      901 qhrqkhtang gqgrcpvcqr qfpswrilrq hmlsqhpdaq qlfkvpggpp rvmgrlgrrt
      961 ykcpycgrvf hrsstdlqkh mwihegvkpf rcpdcpyqcr srnnlnvhrl thsndkphlc
     1021 dqcgkgyksk aalrlhtrth grgvlypcnk ceytatqkch lkrhlethdi irryicehcs
     1081 yssntinymk vhysrkhrge vfnqnsviad pssieqqkdr vykclscsyi fgnmndmkrh
     1141 lrlkhgvcle nldlsveevs aealaseaea aaasaqqemt mepalcedie alevpvgaav
     1201 eeeaaeegtv sliqqmveqg tlaagqevtl qaldaegllt tlnpeaiivr eqdgqplllg
     1261 tqgsylihyv qqdpdgmvlq qgpheqvqqe iiveltp
//
LOCUS       XP_077502472            1297 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113334 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502472
VERSION     XP_077502472.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646346.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1297
                     /product="uncharacterized protein LOC144113334 isoform X1"
                     /calculated_mol_wt=145577
     Region          453..473
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(453,456,469,473)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(458,460,462,464..465,468..469,472,486,492..493,
                     496..497,500,513,515,517,519..520,523..524,527)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          479..503
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          481..501
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          508..528
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          535..553
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          582..602
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(582,585,598,602)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          613..633
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          830..850
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(830,833,846,850)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(835,837,839,841..842,845..846,849,863,865,869..870,
                     873..874,878,891,893,895,897..898,901..902,905)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          858..879
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          886..906
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          915..936
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          963..984
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(963,966,980,984)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(968,970,972,974,976,979..980,983,997,999,1003..1004,
                     1007..1008,1011,1025,1027,1029,1031..1032,1035..1036,1039)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          976..1001
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          992..1012
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1020..1040
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1048..1068
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1076..1092
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1124..1143
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1297
                     /gene="LOC144113334"
                     /coded_by="XM_077646346.1:179..4072"
                     /db_xref="GeneID:144113334"
ORIGIN      
        1 mdvficgvch mgfhdigkfv ehknslgsvs cghctaifht qdeldqhalq ehptetqgla
       61 pkeedmlleq svevdpssee gdnnitaaid sattadsqti vvtegfsfea tensvvsdif
      121 acslcqcfal seeemvnhvn echqcnvskd seeapssyyr lqqlaptvvt tpgedkrtlr
      181 mlpvdveepp tqpttlhlvv etssppappk rrrgrprkmd qlqrpvpavv kpeeepdapl
      241 rgqdglfhcs rckrafcker hilghrcmak gdyvdsydkv gvdqkdeeng lvpdeeakda
      301 dttedfrlhr tserepwary rvrrggfrgg rrggisrgtp gspgpessag pgeankenlv
      361 gqdqaaadka aeggaeeaee lpprklhwre dprhvpvfsr deerlafeeh lnrvdlscvd
      421 hlytqhqvaq eitcppgtrp apkevcdlhv fscnvckkvf ktqshmrlhc lihtdlkpft
      481 chqcdfstna kgnlythmrk htgnyfkcsl cdfrscnrsh maeheathst vrqrcelcsn
      541 dyntikslvn hvrkyhtspa gkrylasfqs kqlqnmavlh ichlcnrkfk kkvdrdrhlf
      601 vhnvpeppta yqcglcgyqa srktyldnhy rkhrlvyvcs lcyglhlsat alrahlqkeh
      661 lekqaaeneg elksessmec fvaengtvst aeetvseglq ttseqsdsse ptqttvlsei
      721 ateseataea alsndatles lfadsigqsw ylpepdgitn syanipqelt egsqennsds
      781 psdsqassaq psgplsssva gmwslsqlhy kqlsmevysk irevfgeeec pdcgrlfhsr
      841 idldphrlth tedkpfkcvk cpyssgskdn lkrhqetahe grtfpcercd fvaqsrsslf
      901 qhrqkhtang gqgrcpvcqr qfpswrilrq hmlsqhpdaq qlfkvpggpp rvmgrlgrrt
      961 ykcpycgrvf hrsstdlqkh mwihegvkpf rcpdcpyqcr srnnlnvhrl thsndkphlc
     1021 dqcgkgyksk aalrlhtrth grgvlypcnk ceytatqkch lkrhlethdi irryicehcs
     1081 yssntinymk vhysrkhrge vfnqnsviad pssieqqkdr vykclscsyi fgnmndmkrh
     1141 lrlkhgvcle nldlsveevs aealaseaea aaasaqqemt mepalcedie alevpvgaav
     1201 eeeaaeegtv sliqqmveqg tlaagqevtl qaldaegllt tlnpeaiivr eqdgqplllg
     1261 tqgsylihyv qqdpdgmvlq qgpheqvqqe iiveltp
//
LOCUS       XP_077502473            1292 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113334 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502473
VERSION     XP_077502473.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646347.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1292
                     /product="uncharacterized protein LOC144113334 isoform X2"
                     /calculated_mol_wt=145078
     Region          448..468
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(448,451,464,468)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(453,455,457,459..460,463..464,467,481,483,487..488,
                     491..492,495,508,510,512,514..515,518..519,522)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          474..498
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          476..496
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          503..523
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          530..548
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          577..597
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(577,580,593,597)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          608..628
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          825..845
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(825,828,841,845)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(830,832,834,836..837,840..841,844,858,860,864..865,
                     868..869,873,886,888,890,892..893,896..897,900)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          853..874
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          881..901
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          910..931
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          958..979
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(958,961,975,979)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(963,965,967,969,971,974..975,978,992,994,998..999,
                     1002..1003,1006,1020,1022,1024,1026..1027,1030..1031,1034)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          971..996
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          987..1007
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1015..1035
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1043..1063
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1071..1087
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1119..1138
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1292
                     /gene="LOC144113334"
                     /coded_by="XM_077646347.1:189..4067"
                     /db_xref="GeneID:144113334"
ORIGIN      
        1 mdvficgvch mgfhdigkfv ehknslgsvs cghctaifht qdeldqhalq ehptetqgla
       61 pkeedmlleq svevdpssee gdnnitaaid sattadsqti vvtegfsfea tensvvsdif
      121 acslcqcfal seeemvnhvn echqcnvskd seeapssyyr lqqlaptvvt tpgedkrtlr
      181 mlpvdveepp tqpttlhlvv etssppappk rrrgrprkmd qlqrpvpavv kpeeepdapl
      241 rgqdglfhcs rckrafcker hilghrcmak gdyvdsydkk deenglvpde eakdadtted
      301 frlhrtsere pwaryrvrrg gfrggrrggi srgtpgspgp essagpgean kenlvgqdqa
      361 aadkaaegga eeaeelpprk lhwredprhv pvfsrdeerl afeehlnrvd lscvdhlytq
      421 hqvaqeitcp pgtrpapkev cdlhvfscnv ckkvfktqsh mrlhclihtd lkpftchqcd
      481 fstnakgnly thmrkhtgny fkcslcdfrs cnrshmaehe athstvrqrc elcsndynti
      541 kslvnhvrky htspagkryl asfqskqlqn mavlhichlc nrkfkkkvdr drhlfvhnvp
      601 epptayqcgl cgyqasrkty ldnhyrkhrl vyvcslcygl hlsatalrah lqkehlekqa
      661 aenegelkse ssmecfvaen gtvstaeetv seglqttseq sdsseptqtt vlseiatese
      721 ataeaalsnd atleslfads igqswylpep dgitnsyani pqeltegsqe nnsdspsdsq
      781 assaqpsgpl sssvagmwsl sqlhykqlsm evyskirevf geeecpdcgr lfhsridldp
      841 hrlthtedkp fkcvkcpyss gskdnlkrhq etahegrtfp cercdfvaqs rsslfqhrqk
      901 htanggqgrc pvcqrqfpsw rilrqhmlsq hpdaqqlfkv pggpprvmgr lgrrtykcpy
      961 cgrvfhrsst dlqkhmwihe gvkpfrcpdc pyqcrsrnnl nvhrlthsnd kphlcdqcgk
     1021 gykskaalrl htrthgrgvl ypcnkceyta tqkchlkrhl ethdiirryi cehcsyssnt
     1081 inymkvhysr khrgevfnqn sviadpssie qqkdrvykcl scsyifgnmn dmkrhlrlkh
     1141 gvclenldls veevsaeala seaeaaaasa qqemtmepal cediealevp vgaaveeeaa
     1201 eegtvsliqq mveqgtlaag qevtlqalda egllttlnpe aiivreqdgq plllgtqgsy
     1261 lihyvqqdpd gmvlqqgphe qvqqeiivel tp
//
LOCUS       XP_077502474            1292 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113334 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502474
VERSION     XP_077502474.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646348.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1292
                     /product="uncharacterized protein LOC144113334 isoform X2"
                     /calculated_mol_wt=145078
     Region          448..468
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(448,451,464,468)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(453,455,457,459..460,463..464,467,481,483,487..488,
                     491..492,495,508,510,512,514..515,518..519,522)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          474..498
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          476..496
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          503..523
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          530..548
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          577..597
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(577,580,593,597)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          608..628
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          825..845
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(825,828,841,845)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(830,832,834,836..837,840..841,844,858,860,864..865,
                     868..869,873,886,888,890,892..893,896..897,900)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          853..874
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          881..901
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          910..931
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          958..979
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(958,961,975,979)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(963,965,967,969,971,974..975,978,992,994,998..999,
                     1002..1003,1006,1020,1022,1024,1026..1027,1030..1031,1034)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          971..996
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          987..1007
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1015..1035
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1043..1063
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1071..1087
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1119..1138
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1292
                     /gene="LOC144113334"
                     /coded_by="XM_077646348.1:243..4121"
                     /db_xref="GeneID:144113334"
ORIGIN      
        1 mdvficgvch mgfhdigkfv ehknslgsvs cghctaifht qdeldqhalq ehptetqgla
       61 pkeedmlleq svevdpssee gdnnitaaid sattadsqti vvtegfsfea tensvvsdif
      121 acslcqcfal seeemvnhvn echqcnvskd seeapssyyr lqqlaptvvt tpgedkrtlr
      181 mlpvdveepp tqpttlhlvv etssppappk rrrgrprkmd qlqrpvpavv kpeeepdapl
      241 rgqdglfhcs rckrafcker hilghrcmak gdyvdsydkk deenglvpde eakdadtted
      301 frlhrtsere pwaryrvrrg gfrggrrggi srgtpgspgp essagpgean kenlvgqdqa
      361 aadkaaegga eeaeelpprk lhwredprhv pvfsrdeerl afeehlnrvd lscvdhlytq
      421 hqvaqeitcp pgtrpapkev cdlhvfscnv ckkvfktqsh mrlhclihtd lkpftchqcd
      481 fstnakgnly thmrkhtgny fkcslcdfrs cnrshmaehe athstvrqrc elcsndynti
      541 kslvnhvrky htspagkryl asfqskqlqn mavlhichlc nrkfkkkvdr drhlfvhnvp
      601 epptayqcgl cgyqasrkty ldnhyrkhrl vyvcslcygl hlsatalrah lqkehlekqa
      661 aenegelkse ssmecfvaen gtvstaeetv seglqttseq sdsseptqtt vlseiatese
      721 ataeaalsnd atleslfads igqswylpep dgitnsyani pqeltegsqe nnsdspsdsq
      781 assaqpsgpl sssvagmwsl sqlhykqlsm evyskirevf geeecpdcgr lfhsridldp
      841 hrlthtedkp fkcvkcpyss gskdnlkrhq etahegrtfp cercdfvaqs rsslfqhrqk
      901 htanggqgrc pvcqrqfpsw rilrqhmlsq hpdaqqlfkv pggpprvmgr lgrrtykcpy
      961 cgrvfhrsst dlqkhmwihe gvkpfrcpdc pyqcrsrnnl nvhrlthsnd kphlcdqcgk
     1021 gykskaalrl htrthgrgvl ypcnkceyta tqkchlkrhl ethdiirryi cehcsyssnt
     1081 inymkvhysr khrgevfnqn sviadpssie qqkdrvykcl scsyifgnmn dmkrhlrlkh
     1141 gvclenldls veevsaeala seaeaaaasa qqemtmepal cediealevp vgaaveeeaa
     1201 eegtvsliqq mveqgtlaag qevtlqalda egllttlnpe aiivreqdgq plllgtqgsy
     1261 lihyvqqdpd gmvlqqgphe qvqqeiivel tp
//
LOCUS       XP_077502475            1292 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113334 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502475
VERSION     XP_077502475.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646349.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1292
                     /product="uncharacterized protein LOC144113334 isoform X2"
                     /calculated_mol_wt=145078
     Region          448..468
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(448,451,464,468)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(453,455,457,459..460,463..464,467,481,483,487..488,
                     491..492,495,508,510,512,514..515,518..519,522)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          474..498
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          476..496
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          503..523
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          530..548
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          577..597
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(577,580,593,597)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          608..628
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          825..845
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(825,828,841,845)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(830,832,834,836..837,840..841,844,858,860,864..865,
                     868..869,873,886,888,890,892..893,896..897,900)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          853..874
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          881..901
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          910..931
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          958..979
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(958,961,975,979)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(963,965,967,969,971,974..975,978,992,994,998..999,
                     1002..1003,1006,1020,1022,1024,1026..1027,1030..1031,1034)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          971..996
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          987..1007
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1015..1035
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1043..1063
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1071..1087
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1119..1138
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1292
                     /gene="LOC144113334"
                     /coded_by="XM_077646349.1:351..4229"
                     /db_xref="GeneID:144113334"
ORIGIN      
        1 mdvficgvch mgfhdigkfv ehknslgsvs cghctaifht qdeldqhalq ehptetqgla
       61 pkeedmlleq svevdpssee gdnnitaaid sattadsqti vvtegfsfea tensvvsdif
      121 acslcqcfal seeemvnhvn echqcnvskd seeapssyyr lqqlaptvvt tpgedkrtlr
      181 mlpvdveepp tqpttlhlvv etssppappk rrrgrprkmd qlqrpvpavv kpeeepdapl
      241 rgqdglfhcs rckrafcker hilghrcmak gdyvdsydkk deenglvpde eakdadtted
      301 frlhrtsere pwaryrvrrg gfrggrrggi srgtpgspgp essagpgean kenlvgqdqa
      361 aadkaaegga eeaeelpprk lhwredprhv pvfsrdeerl afeehlnrvd lscvdhlytq
      421 hqvaqeitcp pgtrpapkev cdlhvfscnv ckkvfktqsh mrlhclihtd lkpftchqcd
      481 fstnakgnly thmrkhtgny fkcslcdfrs cnrshmaehe athstvrqrc elcsndynti
      541 kslvnhvrky htspagkryl asfqskqlqn mavlhichlc nrkfkkkvdr drhlfvhnvp
      601 epptayqcgl cgyqasrkty ldnhyrkhrl vyvcslcygl hlsatalrah lqkehlekqa
      661 aenegelkse ssmecfvaen gtvstaeetv seglqttseq sdsseptqtt vlseiatese
      721 ataeaalsnd atleslfads igqswylpep dgitnsyani pqeltegsqe nnsdspsdsq
      781 assaqpsgpl sssvagmwsl sqlhykqlsm evyskirevf geeecpdcgr lfhsridldp
      841 hrlthtedkp fkcvkcpyss gskdnlkrhq etahegrtfp cercdfvaqs rsslfqhrqk
      901 htanggqgrc pvcqrqfpsw rilrqhmlsq hpdaqqlfkv pggpprvmgr lgrrtykcpy
      961 cgrvfhrsst dlqkhmwihe gvkpfrcpdc pyqcrsrnnl nvhrlthsnd kphlcdqcgk
     1021 gykskaalrl htrthgrgvl ypcnkceyta tqkchlkrhl ethdiirryi cehcsyssnt
     1081 inymkvhysr khrgevfnqn sviadpssie qqkdrvykcl scsyifgnmn dmkrhlrlkh
     1141 gvclenldls veevsaeala seaeaaaasa qqemtmepal cediealevp vgaaveeeaa
     1201 eegtvsliqq mveqgtlaag qevtlqalda egllttlnpe aiivreqdgq plllgtqgsy
     1261 lihyvqqdpd gmvlqqgphe qvqqeiivel tp
//
LOCUS       XP_077502476            1292 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113334 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502476
VERSION     XP_077502476.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646350.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1292
                     /product="uncharacterized protein LOC144113334 isoform X2"
                     /calculated_mol_wt=145078
     Region          448..468
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(448,451,464,468)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(453,455,457,459..460,463..464,467,481,483,487..488,
                     491..492,495,508,510,512,514..515,518..519,522)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          474..498
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          476..496
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          503..523
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          530..548
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          577..597
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(577,580,593,597)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          608..628
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          825..845
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(825,828,841,845)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(830,832,834,836..837,840..841,844,858,860,864..865,
                     868..869,873,886,888,890,892..893,896..897,900)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          853..874
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          881..901
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          910..931
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          958..979
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(958,961,975,979)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(963,965,967,969,971,974..975,978,992,994,998..999,
                     1002..1003,1006,1020,1022,1024,1026..1027,1030..1031,1034)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          971..996
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          987..1007
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1015..1035
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1043..1063
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1071..1087
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1119..1138
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1292
                     /gene="LOC144113334"
                     /coded_by="XM_077646350.1:195..4073"
                     /db_xref="GeneID:144113334"
ORIGIN      
        1 mdvficgvch mgfhdigkfv ehknslgsvs cghctaifht qdeldqhalq ehptetqgla
       61 pkeedmlleq svevdpssee gdnnitaaid sattadsqti vvtegfsfea tensvvsdif
      121 acslcqcfal seeemvnhvn echqcnvskd seeapssyyr lqqlaptvvt tpgedkrtlr
      181 mlpvdveepp tqpttlhlvv etssppappk rrrgrprkmd qlqrpvpavv kpeeepdapl
      241 rgqdglfhcs rckrafcker hilghrcmak gdyvdsydkk deenglvpde eakdadtted
      301 frlhrtsere pwaryrvrrg gfrggrrggi srgtpgspgp essagpgean kenlvgqdqa
      361 aadkaaegga eeaeelpprk lhwredprhv pvfsrdeerl afeehlnrvd lscvdhlytq
      421 hqvaqeitcp pgtrpapkev cdlhvfscnv ckkvfktqsh mrlhclihtd lkpftchqcd
      481 fstnakgnly thmrkhtgny fkcslcdfrs cnrshmaehe athstvrqrc elcsndynti
      541 kslvnhvrky htspagkryl asfqskqlqn mavlhichlc nrkfkkkvdr drhlfvhnvp
      601 epptayqcgl cgyqasrkty ldnhyrkhrl vyvcslcygl hlsatalrah lqkehlekqa
      661 aenegelkse ssmecfvaen gtvstaeetv seglqttseq sdsseptqtt vlseiatese
      721 ataeaalsnd atleslfads igqswylpep dgitnsyani pqeltegsqe nnsdspsdsq
      781 assaqpsgpl sssvagmwsl sqlhykqlsm evyskirevf geeecpdcgr lfhsridldp
      841 hrlthtedkp fkcvkcpyss gskdnlkrhq etahegrtfp cercdfvaqs rsslfqhrqk
      901 htanggqgrc pvcqrqfpsw rilrqhmlsq hpdaqqlfkv pggpprvmgr lgrrtykcpy
      961 cgrvfhrsst dlqkhmwihe gvkpfrcpdc pyqcrsrnnl nvhrlthsnd kphlcdqcgk
     1021 gykskaalrl htrthgrgvl ypcnkceyta tqkchlkrhl ethdiirryi cehcsyssnt
     1081 inymkvhysr khrgevfnqn sviadpssie qqkdrvykcl scsyifgnmn dmkrhlrlkh
     1141 gvclenldls veevsaeala seaeaaaasa qqemtmepal cediealevp vgaaveeeaa
     1201 eegtvsliqq mveqgtlaag qevtlqalda egllttlnpe aiivreqdgq plllgtqgsy
     1261 lihyvqqdpd gmvlqqgphe qvqqeiivel tp
//
LOCUS       XP_077502479            1221 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113334 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502479
VERSION     XP_077502479.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646353.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1221
                     /product="uncharacterized protein LOC144113334 isoform X3"
                     /calculated_mol_wt=136611
     Region          453..473
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(453,456,469,473)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(458,460,462,464..465,468..469,472,486,492..493,
                     496..497,500,513,515,517,519..520,523..524,527)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          479..503
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          481..501
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          508..528
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          535..553
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          582..602
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(582,585,598,602)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          613..633
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          830..850
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          887..908
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(887,890,904,908)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(892,894,896,898,900,903..904,907,921,923,927..928,
                     931..932,935,949,951,953,955..956,959..960,963)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          900..925
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          <910..964
                     /region_name="SFP1"
                     /note="Putative transcriptional repressor regulating G2/M
                     transition [Transcription / Cell division and chromosome
                     partitioning]; COG5189"
                     /db_xref="CDD:227516"
     Region          916..936
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          944..964
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          972..992
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1000..1016
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1048..1067
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1221
                     /gene="LOC144113334"
                     /coded_by="XM_077646353.1:189..3854"
                     /db_xref="GeneID:144113334"
ORIGIN      
        1 mdvficgvch mgfhdigkfv ehknslgsvs cghctaifht qdeldqhalq ehptetqgla
       61 pkeedmlleq svevdpssee gdnnitaaid sattadsqti vvtegfsfea tensvvsdif
      121 acslcqcfal seeemvnhvn echqcnvskd seeapssyyr lqqlaptvvt tpgedkrtlr
      181 mlpvdveepp tqpttlhlvv etssppappk rrrgrprkmd qlqrpvpavv kpeeepdapl
      241 rgqdglfhcs rckrafcker hilghrcmak gdyvdsydkv gvdqkdeeng lvpdeeakda
      301 dttedfrlhr tserepwary rvrrggfrgg rrggisrgtp gspgpessag pgeankenlv
      361 gqdqaaadka aeggaeeaee lpprklhwre dprhvpvfsr deerlafeeh lnrvdlscvd
      421 hlytqhqvaq eitcppgtrp apkevcdlhv fscnvckkvf ktqshmrlhc lihtdlkpft
      481 chqcdfstna kgnlythmrk htgnyfkcsl cdfrscnrsh maeheathst vrqrcelcsn
      541 dyntikslvn hvrkyhtspa gkrylasfqs kqlqnmavlh ichlcnrkfk kkvdrdrhlf
      601 vhnvpeppta yqcglcgyqa srktyldnhy rkhrlvyvcs lcyglhlsat alrahlqkeh
      661 lekqaaeneg elksessmec fvaengtvst aeetvseglq ttseqsdsse ptqttvlsei
      721 ateseataea alsndatles lfadsigqsw ylpepdgitn syanipqelt egsqennsds
      781 psdsqassaq psgplsssva gmwslsqlhy kqlsmevysk irevfgeeec pdcgrlfhsr
      841 idldphrlth tedkpfkcvk cpyssgskvp ggpprvmgrl grrtykcpyc grvfhrsstd
      901 lqkhmwiheg vkpfrcpdcp yqcrsrnnln vhrlthsndk phlcdqcgkg ykskaalrlh
      961 trthgrgvly pcnkceytat qkchlkrhle thdiirryic ehcsyssnti nymkvhysrk
     1021 hrgevfnqns viadpssieq qkdrvykcls csyifgnmnd mkrhlrlkhg vclenldlsv
     1081 eevsaealas eaeaaaasaq qemtmepalc ediealevpv gaaveeeaae egtvsliqqm
     1141 veqgtlaagq evtlqaldae gllttlnpea iivreqdgqp lllgtqgsyl ihyvqqdpdg
     1201 mvlqqgpheq vqqeiivelt p
//
LOCUS       XP_077502480            1216 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113334 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077502480
VERSION     XP_077502480.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646354.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1216
                     /product="uncharacterized protein LOC144113334 isoform X4"
                     /calculated_mol_wt=136112
     Region          448..468
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(448,451,464,468)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(453,455,457,459..460,463..464,467,481,487..488,
                     491..492,495,508,510,512,514..515,518..519,522)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          474..498
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          476..496
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          503..523
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          530..548
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          577..597
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(577,580,593,597)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          608..628
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          825..845
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          882..903
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(882,885,899,903)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(887,889,891,893,895,898..899,902,916,918,922..923,
                     926..927,930,944,946,948,950..951,954..955,958)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          895..920
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          <905..959
                     /region_name="SFP1"
                     /note="Putative transcriptional repressor regulating G2/M
                     transition [Transcription / Cell division and chromosome
                     partitioning]; COG5189"
                     /db_xref="CDD:227516"
     Region          911..931
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          939..959
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          967..987
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          995..1011
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1043..1062
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1216
                     /gene="LOC144113334"
                     /coded_by="XM_077646354.1:189..3839"
                     /db_xref="GeneID:144113334"
ORIGIN      
        1 mdvficgvch mgfhdigkfv ehknslgsvs cghctaifht qdeldqhalq ehptetqgla
       61 pkeedmlleq svevdpssee gdnnitaaid sattadsqti vvtegfsfea tensvvsdif
      121 acslcqcfal seeemvnhvn echqcnvskd seeapssyyr lqqlaptvvt tpgedkrtlr
      181 mlpvdveepp tqpttlhlvv etssppappk rrrgrprkmd qlqrpvpavv kpeeepdapl
      241 rgqdglfhcs rckrafcker hilghrcmak gdyvdsydkk deenglvpde eakdadtted
      301 frlhrtsere pwaryrvrrg gfrggrrggi srgtpgspgp essagpgean kenlvgqdqa
      361 aadkaaegga eeaeelpprk lhwredprhv pvfsrdeerl afeehlnrvd lscvdhlytq
      421 hqvaqeitcp pgtrpapkev cdlhvfscnv ckkvfktqsh mrlhclihtd lkpftchqcd
      481 fstnakgnly thmrkhtgny fkcslcdfrs cnrshmaehe athstvrqrc elcsndynti
      541 kslvnhvrky htspagkryl asfqskqlqn mavlhichlc nrkfkkkvdr drhlfvhnvp
      601 epptayqcgl cgyqasrkty ldnhyrkhrl vyvcslcygl hlsatalrah lqkehlekqa
      661 aenegelkse ssmecfvaen gtvstaeetv seglqttseq sdsseptqtt vlseiatese
      721 ataeaalsnd atleslfads igqswylpep dgitnsyani pqeltegsqe nnsdspsdsq
      781 assaqpsgpl sssvagmwsl sqlhykqlsm evyskirevf geeecpdcgr lfhsridldp
      841 hrlthtedkp fkcvkcpyss gskvpggppr vmgrlgrrty kcpycgrvfh rsstdlqkhm
      901 wihegvkpfr cpdcpyqcrs rnnlnvhrlt hsndkphlcd qcgkgykska alrlhtrthg
      961 rgvlypcnkc eytatqkchl krhlethdii rryicehcsy ssntinymkv hysrkhrgev
     1021 fnqnsviadp ssieqqkdrv ykclscsyif gnmndmkrhl rlkhgvclen ldlsveevsa
     1081 ealaseaeaa aasaqqemtm epalcediea levpvgaave eeaaeegtvs liqqmveqgt
     1141 laagqevtlq aldaeglltt lnpeaiivre qdgqplllgt qgsylihyvq qdpdgmvlqq
     1201 gpheqvqqei iveltp
//
LOCUS       XP_077502481             707 aa            linear   INV 25-NOV-2025
DEFINITION  epithelial splicing regulatory protein fusilli isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077502481
VERSION     XP_077502481.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646355.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..707
                     /product="epithelial splicing regulatory protein fusilli
                     isoform X1"
                     /calculated_mol_wt=78528
     Region          25..168
                     /region_name="DnaQ_like_exo"
                     /note="DnaQ-like (or DEDD) 3'-5' exonuclease domain
                     superfamily; cl10012"
                     /db_xref="CDD:447876"
     Region          213..305
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          306..414
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          460..543
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          630..705
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..707
                     /gene="fus"
                     /coded_by="XM_077646355.1:795..2918"
                     /db_xref="GeneID:144113336"
ORIGIN      
        1 maatrfvvvv hlvtagqqgd algsdeqdtv vfswlvvdla nnkvvavqqn lvkprscdvn
       61 envisescrt eygiseeqlk navpleqvid qfsqwararl eaeaggqfyf vtdgqlplrq
      121 alhpeavrhg illpdafyhf fdlrkefqaf ygkavdsvqd mlaylslepd asaeivtrhv
      181 qdmakvilrl nmdghkftep eeisqrlesg icsktdvvds ntvvrarglp wqssdqdiak
      241 ffsglnivkg gvalclspqg rrngealvrf ensehrdmal krhkhhigqr yievyratge
      301 dfvnvaggnn neahsflsrg gqvivrmrgl pydctakqvv effassgdgs eagsephtsc
      361 pvmddedgvl fvrkpdgrat gdafvlfdse evasralhkh rqligsryie lfrsttaevq
      421 qvlnrtmdpr tydtaqplia slpqvpllpq qmlpsrssrk dcirlrglpy eaqvetilef
      481 lgehaqaivy qgvhmvynaq gqpsgeafiq mdsedsafla alqrhhryim gkkpryievf
      541 qcsiddmnlv ltggipvqrp llspaagaml ppapygaypy vqppapvlpa atmttlaprv
      601 styyppifyw sypsppvspt tyyphsagpt mvilrglpys stpqdiltff qgfpeltadc
      661 iqiqrnsdgr pngeavvtfp sraeaeraiq eknrqnigsr yielfma
//
LOCUS       XP_077502482             683 aa            linear   INV 25-NOV-2025
DEFINITION  epithelial splicing regulatory protein fusilli isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077502482
VERSION     XP_077502482.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646356.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..683
                     /product="epithelial splicing regulatory protein fusilli
                     isoform X2"
                     /calculated_mol_wt=75820
     Region          25..168
                     /region_name="DnaQ_like_exo"
                     /note="DnaQ-like (or DEDD) 3'-5' exonuclease domain
                     superfamily; cl10012"
                     /db_xref="CDD:447876"
     Region          213..305
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          306..414
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          460..543
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          <632..681
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..683
                     /gene="fus"
                     /coded_by="XM_077646356.1:782..2833"
                     /db_xref="GeneID:144113336"
ORIGIN      
        1 maatrfvvvv hlvtagqqgd algsdeqdtv vfswlvvdla nnkvvavqqn lvkprscdvn
       61 envisescrt eygiseeqlk navpleqvid qfsqwararl eaeaggqfyf vtdgqlplrq
      121 alhpeavrhg illpdafyhf fdlrkefqaf ygkavdsvqd mlaylslepd asaeivtrhv
      181 qdmakvilrl nmdghkftep eeisqrlesg icsktdvvds ntvvrarglp wqssdqdiak
      241 ffsglnivkg gvalclspqg rrngealvrf ensehrdmal krhkhhigqr yievyratge
      301 dfvnvaggnn neahsflsrg gqvivrmrgl pydctakqvv effassgdgs eagsephtsc
      361 pvmddedgvl fvrkpdgrat gdafvlfdse evasralhkh rqligsryie lfrsttaevq
      421 qvlnrtmdpr tydtaqplia slpqvpllpq qmlpsrssrk dcirlrglpy eaqvetilef
      481 lgehaqaivy qgvhmvynaq gqpsgeafiq mdsedsafla alqrhhryim gkkpryievf
      541 qcsiddmnlv ltggipvqrp llspaagaml ppapygaypy vqppapvlpa atmttlaprv
      601 styyppifyw sypsppvspt tyyphsagpt mltadciqiq rnsdgrpnge avvtfpsrae
      661 aeraiqeknr qnigsryiel fma
//
LOCUS       XP_077502483             395 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113337 [Amblyomma americanum].
ACCESSION   XP_077502483
VERSION     XP_077502483.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646357.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..395
                     /product="uncharacterized protein LOC144113337"
                     /calculated_mol_wt=44837
     Region          138..172
                     /region_name="F-box_SF"
                     /note="F-box domain superfamily; cd09917"
                     /db_xref="CDD:438852"
     Site            order(140..141,143..145,148..149,152,163..165,167,170)
                     /site_type="other"
                     /note="F-box motif"
                     /db_xref="CDD:438852"
     Site            order(140,144,147..148,151..152,156,158..159,163..165,167)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438852"
     Region          237..382
                     /region_name="UEV_Morgue-like"
                     /note="ubiquitin E2 variant (UEV) domain of Drosophila
                     melanogaster Morgue and related proteins; cd23826"
                     /db_xref="CDD:467436"
     CDS             1..395
                     /gene="LOC144113337"
                     /coded_by="XM_077646357.1:268..1455"
                     /db_xref="GeneID:144113337"
ORIGIN      
        1 maavncnhqr stvsvrrpad grleemlqng hnifqfkqtv chacngfygp cfghplcctc
       61 haflypdvls mqdgdqgwks dsedsgndep adfydmvkhn verpklpppg pkqdrlaeci
      121 salsspkpqe stpdgvlarl plevlslifs rlddvslwhc sqvcrrwhhi leceltenhw
      181 alfikhrwpl fkpprkvasw rdlysrlvvs apcrrclqel regprsdtca wgpecswrtv
      241 rlqqelrqlq teppegigal pldplcahwq aaiagppdsp yegglfhlfl qipsgypmkp
      301 pvvkfltkif hpnisrhgdv gidslhhnws laltiskvli siqslltdpy clvcmepevg
      361 rlyledrlgf ertarlwtlr yamhgdpvny fahpp
//
LOCUS       XP_077502484             395 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113337 [Amblyomma americanum].
ACCESSION   XP_077502484
VERSION     XP_077502484.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646358.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..395
                     /product="uncharacterized protein LOC144113337"
                     /calculated_mol_wt=44837
     Region          138..172
                     /region_name="F-box_SF"
                     /note="F-box domain superfamily; cd09917"
                     /db_xref="CDD:438852"
     Site            order(140..141,143..145,148..149,152,163..165,167,170)
                     /site_type="other"
                     /note="F-box motif"
                     /db_xref="CDD:438852"
     Site            order(140,144,147..148,151..152,156,158..159,163..165,167)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438852"
     Region          237..382
                     /region_name="UEV_Morgue-like"
                     /note="ubiquitin E2 variant (UEV) domain of Drosophila
                     melanogaster Morgue and related proteins; cd23826"
                     /db_xref="CDD:467436"
     CDS             1..395
                     /gene="LOC144113337"
                     /coded_by="XM_077646358.1:155..1342"
                     /db_xref="GeneID:144113337"
ORIGIN      
        1 maavncnhqr stvsvrrpad grleemlqng hnifqfkqtv chacngfygp cfghplcctc
       61 haflypdvls mqdgdqgwks dsedsgndep adfydmvkhn verpklpppg pkqdrlaeci
      121 salsspkpqe stpdgvlarl plevlslifs rlddvslwhc sqvcrrwhhi leceltenhw
      181 alfikhrwpl fkpprkvasw rdlysrlvvs apcrrclqel regprsdtca wgpecswrtv
      241 rlqqelrqlq teppegigal pldplcahwq aaiagppdsp yegglfhlfl qipsgypmkp
      301 pvvkfltkif hpnisrhgdv gidslhhnws laltiskvli siqslltdpy clvcmepevg
      361 rlyledrlgf ertarlwtlr yamhgdpvny fahpp
//
LOCUS       XP_077502485             406 aa            linear   INV 25-NOV-2025
DEFINITION  dnaJ homolog subfamily A member 2-like [Amblyomma americanum].
ACCESSION   XP_077502485
VERSION     XP_077502485.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646359.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..406
                     /product="dnaJ homolog subfamily A member 2-like"
                     /calculated_mol_wt=45561
     Region          5..406
                     /region_name="PTZ00037"
                     /note="DnaJ_C chaperone protein; Provisional"
                     /db_xref="CDD:240236"
     CDS             1..406
                     /gene="LOC144113338"
                     /coded_by="XM_077646359.1:89..1309"
                     /db_xref="GeneID:144113338"
ORIGIN      
        1 msdgklydvl gvsrnatdye ikkayrrlak eyhpdknpqe gekfkeisfa yevlsdpkkr
       61 eiyntyglng lkegvhespf atedifshif ggspfggmfg mdgsrrrrqr gedtihplkv
      121 sledlyngkr aklqvdhtvi ckacdgvggr sgavltcqsc rgrgikvtfk higpnmmqqv
      181 qstcpdcrgd gevinekdac ksckgrkvik elkylevdvd kgmrdneriv frgegdqqpg
      241 mepgdiiivl qtkphevfhr dgcdlymths vtltealcgf emvlkhldgr dlvirevpgs
      301 vikprcikgi rgegmplyrn ptekgnlyik fdvdfpenhf adeaklkele allgdrpsgg
      361 lgevpvgehv eevdlhdydp nsqsqrqeay hedeqqprss vecahq
//
LOCUS       XP_077502486            1432 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger protein pebbled [Amblyomma americanum].
ACCESSION   XP_077502486
VERSION     XP_077502486.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646360.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1432
                     /product="zinc finger protein pebbled"
                     /calculated_mol_wt=156396
     Region          <89..138
                     /region_name="PHA00733"
                     /note="hypothetical protein"
                     /db_xref="CDD:177301"
     Region          92..112
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(92,95,108,112)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(97,99,101,103..104,107..108,111,126,128,132..133,
                     136..137,140,154,156,158,160..161,164..165,168)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          121..141
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          133..158
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          149..169
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          203..223
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          472..492
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(472,475,488,492)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(477,479,481,483..484,487..488,491,506,508,512..513,
                     516..517,520,534,536,538,540..541,544..545)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          499..523
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          501..521
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          514..538
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          529..547
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          589..610
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Site            order(594,596,598,600..601,604..605,608,625,627..629,
                     631..632,636)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275370"
     Region          620..636
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          <646..753
                     /region_name="LGT"
                     /note="Prolipoprotein diacylglyceryl transferase; cl00478"
                     /db_xref="CDD:469786"
     Region          1059..1079
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1059,1062,1075,1079)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1064,1066,1068,1070..1071,1074..1075,1078,1092,1094,
                     1098..1099,1102..1103,1107,1131,1134,1136,1138..1139,
                     1142..1143,1146)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1071..1096
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1087..1108
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1126..1146
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1201..1222
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          1228..1249
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1228,1231,1244,1249)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1233,1235,1237,1239..1240,1243..1244,1247,1290,1292,
                     1296..1297,1300..1301,1305,1318,1320,1322,1324..1325,
                     1328..1329,1332)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1285..1306
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1313..1333
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1432
                     /gene="peb"
                     /coded_by="XM_077646360.1:343..4641"
                     /db_xref="GeneID:144113339"
ORIGIN      
        1 mngdpgklsp vhsikddgag dtdresgtdd qihlgrattp larpnsvpsk lgssgtesst
       61 nesphiqqdg dlqrdaitgr rkhsvvegph icpvcddvlg sshaltlhir qhnptdhsht
      121 crlcgktlss assldrhmlv hsgerpfkca vcqmafttng nmhrhmrtha gpeepegpar
      181 rglkrppevd tpplsplpeq vscpvcgkaf lcraglashm vthphdpiqc tqccevtasy
      241 aayldhrcgp qevapqgpgf hdltfvdfst gkfsliaksl cesqarrpss afhtfecnlc
      301 krafpcssal rlhaqahgrg tfcpscscdf psprfleahq lkhrraeqcg ppacskedfl
      361 allelqtras saaleeavph gkeehfesya yfnggtprpe keaghdfadi qsiisvtska
      421 plaatspgqs gasprpgspl eadsaspdgg kpeegtrdes naeedgqpqf gcklchmsfa
      481 slgllrkhgq lhshgttpya cnvcsytsmd kstlirhlrt hngerpfqca ickyafttka
      541 ncerhvrkrh kkltkgevrg amqynphmae rstssetlap evgsletvck ycsvdfkfnr
      601 vlrhhlrslh nscsrkpfcc nicklgfstk nncirhalkq hpdmrdrlaq vvaakplals
      661 dssedltags ledspqktsp adataplsqp papkeplpee pardqcldls sqnppegkas
      721 seaaeplnlt lkpaqegatg esdannndeq viaakslvsl selpppqeep ldlavhaidl
      781 swrnsispel aqgthsaspc appppplppp sqllqpsski qlppktpplg rppvnglpsa
      841 rnptilesss asddiykmtf kmvndklspk llpknqrsfs cvycsagftl ksnmerhikr
      901 khpeharptr srnfiptmss pgsskpfsst lsdetrtalr vvlsskanka adaaesmpss
      961 asqeqqakae skdehrsved lssrtvengs degsadlasv ssvihtansq vfqqylldek
     1021 glpeetqeta tgecsesdgg ehrekkrssy tdspnsvacp ycsrkfpwts slrrhiltht
     1081 gqkpfkcerc piwfttksnc erhyvrkhgr hgdhltrcvp erpfkcnvcp sstfstrgnl
     1141 rkhyyikhws rngadksarm paegdsephn edsldamdda gegssdngse dphqcpdrlh
     1201 cpcceavfst aseiehhvgq lievpyrchq cerafvqrad clahlesdht elqlpadieh
     1261 evvaahmksi ipgtsedygl criacvfclr rfvstdslkk hvlgchtgdt facdtchqkl
     1321 psknsitrhk rkhhdlllse pgdsseeeqp nsedqpqtnh khsskkllll gkpkkkaada
     1381 aetgstetks plecgngdli qnllgiqdsk iidemlmnpa dsaarllgvk qv
//
LOCUS       XP_077502487            1432 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger protein pebbled [Amblyomma americanum].
ACCESSION   XP_077502487
VERSION     XP_077502487.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646361.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1432
                     /product="zinc finger protein pebbled"
                     /calculated_mol_wt=156396
     Region          <89..138
                     /region_name="PHA00733"
                     /note="hypothetical protein"
                     /db_xref="CDD:177301"
     Region          92..112
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(92,95,108,112)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(97,99,101,103..104,107..108,111,126,128,132..133,
                     136..137,140,154,156,158,160..161,164..165,168)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          121..141
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          133..158
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          149..169
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          203..223
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          472..492
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(472,475,488,492)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(477,479,481,483..484,487..488,491,506,508,512..513,
                     516..517,520,534,536,538,540..541,544..545)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          499..523
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          501..521
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          514..538
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          529..547
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          589..610
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Site            order(594,596,598,600..601,604..605,608,625,627..629,
                     631..632,636)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275370"
     Region          620..636
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          <646..753
                     /region_name="LGT"
                     /note="Prolipoprotein diacylglyceryl transferase; cl00478"
                     /db_xref="CDD:469786"
     Region          1059..1079
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1059,1062,1075,1079)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1064,1066,1068,1070..1071,1074..1075,1078,1092,1094,
                     1098..1099,1102..1103,1107,1131,1134,1136,1138..1139,
                     1142..1143,1146)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1071..1096
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1087..1108
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1126..1146
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1201..1222
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          1228..1249
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1228,1231,1244,1249)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1233,1235,1237,1239..1240,1243..1244,1247,1290,1292,
                     1296..1297,1300..1301,1305,1318,1320,1322,1324..1325,
                     1328..1329,1332)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1285..1306
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1313..1333
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1432
                     /gene="peb"
                     /coded_by="XM_077646361.1:335..4633"
                     /db_xref="GeneID:144113339"
ORIGIN      
        1 mngdpgklsp vhsikddgag dtdresgtdd qihlgrattp larpnsvpsk lgssgtesst
       61 nesphiqqdg dlqrdaitgr rkhsvvegph icpvcddvlg sshaltlhir qhnptdhsht
      121 crlcgktlss assldrhmlv hsgerpfkca vcqmafttng nmhrhmrtha gpeepegpar
      181 rglkrppevd tpplsplpeq vscpvcgkaf lcraglashm vthphdpiqc tqccevtasy
      241 aayldhrcgp qevapqgpgf hdltfvdfst gkfsliaksl cesqarrpss afhtfecnlc
      301 krafpcssal rlhaqahgrg tfcpscscdf psprfleahq lkhrraeqcg ppacskedfl
      361 allelqtras saaleeavph gkeehfesya yfnggtprpe keaghdfadi qsiisvtska
      421 plaatspgqs gasprpgspl eadsaspdgg kpeegtrdes naeedgqpqf gcklchmsfa
      481 slgllrkhgq lhshgttpya cnvcsytsmd kstlirhlrt hngerpfqca ickyafttka
      541 ncerhvrkrh kkltkgevrg amqynphmae rstssetlap evgsletvck ycsvdfkfnr
      601 vlrhhlrslh nscsrkpfcc nicklgfstk nncirhalkq hpdmrdrlaq vvaakplals
      661 dssedltags ledspqktsp adataplsqp papkeplpee pardqcldls sqnppegkas
      721 seaaeplnlt lkpaqegatg esdannndeq viaakslvsl selpppqeep ldlavhaidl
      781 swrnsispel aqgthsaspc appppplppp sqllqpsski qlppktpplg rppvnglpsa
      841 rnptilesss asddiykmtf kmvndklspk llpknqrsfs cvycsagftl ksnmerhikr
      901 khpeharptr srnfiptmss pgsskpfsst lsdetrtalr vvlsskanka adaaesmpss
      961 asqeqqakae skdehrsved lssrtvengs degsadlasv ssvihtansq vfqqylldek
     1021 glpeetqeta tgecsesdgg ehrekkrssy tdspnsvacp ycsrkfpwts slrrhiltht
     1081 gqkpfkcerc piwfttksnc erhyvrkhgr hgdhltrcvp erpfkcnvcp sstfstrgnl
     1141 rkhyyikhws rngadksarm paegdsephn edsldamdda gegssdngse dphqcpdrlh
     1201 cpcceavfst aseiehhvgq lievpyrchq cerafvqrad clahlesdht elqlpadieh
     1261 evvaahmksi ipgtsedygl criacvfclr rfvstdslkk hvlgchtgdt facdtchqkl
     1321 psknsitrhk rkhhdlllse pgdsseeeqp nsedqpqtnh khsskkllll gkpkkkaada
     1381 aetgstetks plecgngdli qnllgiqdsk iidemlmnpa dsaarllgvk qv
//
LOCUS       XP_077502488            1432 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger protein pebbled [Amblyomma americanum].
ACCESSION   XP_077502488
VERSION     XP_077502488.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646362.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1432
                     /product="zinc finger protein pebbled"
                     /calculated_mol_wt=156396
     Region          <89..138
                     /region_name="PHA00733"
                     /note="hypothetical protein"
                     /db_xref="CDD:177301"
     Region          92..112
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(92,95,108,112)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(97,99,101,103..104,107..108,111,126,128,132..133,
                     136..137,140,154,156,158,160..161,164..165,168)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          121..141
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          133..158
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          149..169
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          203..223
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          472..492
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(472,475,488,492)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(477,479,481,483..484,487..488,491,506,508,512..513,
                     516..517,520,534,536,538,540..541,544..545)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          499..523
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          501..521
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          514..538
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          529..547
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          589..610
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Site            order(594,596,598,600..601,604..605,608,625,627..629,
                     631..632,636)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275370"
     Region          620..636
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          <646..753
                     /region_name="LGT"
                     /note="Prolipoprotein diacylglyceryl transferase; cl00478"
                     /db_xref="CDD:469786"
     Region          1059..1079
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1059,1062,1075,1079)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1064,1066,1068,1070..1071,1074..1075,1078,1092,1094,
                     1098..1099,1102..1103,1107,1131,1134,1136,1138..1139,
                     1142..1143,1146)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1071..1096
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1087..1108
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1126..1146
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1201..1222
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          1228..1249
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1228,1231,1244,1249)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1233,1235,1237,1239..1240,1243..1244,1247,1290,1292,
                     1296..1297,1300..1301,1305,1318,1320,1322,1324..1325,
                     1328..1329,1332)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1285..1306
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1313..1333
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1432
                     /gene="peb"
                     /coded_by="XM_077646362.1:269..4567"
                     /db_xref="GeneID:144113339"
ORIGIN      
        1 mngdpgklsp vhsikddgag dtdresgtdd qihlgrattp larpnsvpsk lgssgtesst
       61 nesphiqqdg dlqrdaitgr rkhsvvegph icpvcddvlg sshaltlhir qhnptdhsht
      121 crlcgktlss assldrhmlv hsgerpfkca vcqmafttng nmhrhmrtha gpeepegpar
      181 rglkrppevd tpplsplpeq vscpvcgkaf lcraglashm vthphdpiqc tqccevtasy
      241 aayldhrcgp qevapqgpgf hdltfvdfst gkfsliaksl cesqarrpss afhtfecnlc
      301 krafpcssal rlhaqahgrg tfcpscscdf psprfleahq lkhrraeqcg ppacskedfl
      361 allelqtras saaleeavph gkeehfesya yfnggtprpe keaghdfadi qsiisvtska
      421 plaatspgqs gasprpgspl eadsaspdgg kpeegtrdes naeedgqpqf gcklchmsfa
      481 slgllrkhgq lhshgttpya cnvcsytsmd kstlirhlrt hngerpfqca ickyafttka
      541 ncerhvrkrh kkltkgevrg amqynphmae rstssetlap evgsletvck ycsvdfkfnr
      601 vlrhhlrslh nscsrkpfcc nicklgfstk nncirhalkq hpdmrdrlaq vvaakplals
      661 dssedltags ledspqktsp adataplsqp papkeplpee pardqcldls sqnppegkas
      721 seaaeplnlt lkpaqegatg esdannndeq viaakslvsl selpppqeep ldlavhaidl
      781 swrnsispel aqgthsaspc appppplppp sqllqpsski qlppktpplg rppvnglpsa
      841 rnptilesss asddiykmtf kmvndklspk llpknqrsfs cvycsagftl ksnmerhikr
      901 khpeharptr srnfiptmss pgsskpfsst lsdetrtalr vvlsskanka adaaesmpss
      961 asqeqqakae skdehrsved lssrtvengs degsadlasv ssvihtansq vfqqylldek
     1021 glpeetqeta tgecsesdgg ehrekkrssy tdspnsvacp ycsrkfpwts slrrhiltht
     1081 gqkpfkcerc piwfttksnc erhyvrkhgr hgdhltrcvp erpfkcnvcp sstfstrgnl
     1141 rkhyyikhws rngadksarm paegdsephn edsldamdda gegssdngse dphqcpdrlh
     1201 cpcceavfst aseiehhvgq lievpyrchq cerafvqrad clahlesdht elqlpadieh
     1261 evvaahmksi ipgtsedygl criacvfclr rfvstdslkk hvlgchtgdt facdtchqkl
     1321 psknsitrhk rkhhdlllse pgdsseeeqp nsedqpqtnh khsskkllll gkpkkkaada
     1381 aetgstetks plecgngdli qnllgiqdsk iidemlmnpa dsaarllgvk qv
//
LOCUS       XP_077502489            1432 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger protein pebbled [Amblyomma americanum].
ACCESSION   XP_077502489
VERSION     XP_077502489.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646363.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1432
                     /product="zinc finger protein pebbled"
                     /calculated_mol_wt=156396
     Region          <89..138
                     /region_name="PHA00733"
                     /note="hypothetical protein"
                     /db_xref="CDD:177301"
     Region          92..112
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(92,95,108,112)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(97,99,101,103..104,107..108,111,126,128,132..133,
                     136..137,140,154,156,158,160..161,164..165,168)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          121..141
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          133..158
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          149..169
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          203..223
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          472..492
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(472,475,488,492)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(477,479,481,483..484,487..488,491,506,508,512..513,
                     516..517,520,534,536,538,540..541,544..545)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          499..523
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          501..521
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          514..538
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          529..547
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          589..610
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Site            order(594,596,598,600..601,604..605,608,625,627..629,
                     631..632,636)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275370"
     Region          620..636
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          <646..753
                     /region_name="LGT"
                     /note="Prolipoprotein diacylglyceryl transferase; cl00478"
                     /db_xref="CDD:469786"
     Region          1059..1079
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1059,1062,1075,1079)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1064,1066,1068,1070..1071,1074..1075,1078,1092,1094,
                     1098..1099,1102..1103,1107,1131,1134,1136,1138..1139,
                     1142..1143,1146)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1071..1096
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1087..1108
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1126..1146
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1201..1222
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          1228..1249
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1228,1231,1244,1249)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1233,1235,1237,1239..1240,1243..1244,1247,1290,1292,
                     1296..1297,1300..1301,1305,1318,1320,1322,1324..1325,
                     1328..1329,1332)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1285..1306
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1313..1333
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1432
                     /gene="peb"
                     /coded_by="XM_077646363.1:126..4424"
                     /db_xref="GeneID:144113339"
ORIGIN      
        1 mngdpgklsp vhsikddgag dtdresgtdd qihlgrattp larpnsvpsk lgssgtesst
       61 nesphiqqdg dlqrdaitgr rkhsvvegph icpvcddvlg sshaltlhir qhnptdhsht
      121 crlcgktlss assldrhmlv hsgerpfkca vcqmafttng nmhrhmrtha gpeepegpar
      181 rglkrppevd tpplsplpeq vscpvcgkaf lcraglashm vthphdpiqc tqccevtasy
      241 aayldhrcgp qevapqgpgf hdltfvdfst gkfsliaksl cesqarrpss afhtfecnlc
      301 krafpcssal rlhaqahgrg tfcpscscdf psprfleahq lkhrraeqcg ppacskedfl
      361 allelqtras saaleeavph gkeehfesya yfnggtprpe keaghdfadi qsiisvtska
      421 plaatspgqs gasprpgspl eadsaspdgg kpeegtrdes naeedgqpqf gcklchmsfa
      481 slgllrkhgq lhshgttpya cnvcsytsmd kstlirhlrt hngerpfqca ickyafttka
      541 ncerhvrkrh kkltkgevrg amqynphmae rstssetlap evgsletvck ycsvdfkfnr
      601 vlrhhlrslh nscsrkpfcc nicklgfstk nncirhalkq hpdmrdrlaq vvaakplals
      661 dssedltags ledspqktsp adataplsqp papkeplpee pardqcldls sqnppegkas
      721 seaaeplnlt lkpaqegatg esdannndeq viaakslvsl selpppqeep ldlavhaidl
      781 swrnsispel aqgthsaspc appppplppp sqllqpsski qlppktpplg rppvnglpsa
      841 rnptilesss asddiykmtf kmvndklspk llpknqrsfs cvycsagftl ksnmerhikr
      901 khpeharptr srnfiptmss pgsskpfsst lsdetrtalr vvlsskanka adaaesmpss
      961 asqeqqakae skdehrsved lssrtvengs degsadlasv ssvihtansq vfqqylldek
     1021 glpeetqeta tgecsesdgg ehrekkrssy tdspnsvacp ycsrkfpwts slrrhiltht
     1081 gqkpfkcerc piwfttksnc erhyvrkhgr hgdhltrcvp erpfkcnvcp sstfstrgnl
     1141 rkhyyikhws rngadksarm paegdsephn edsldamdda gegssdngse dphqcpdrlh
     1201 cpcceavfst aseiehhvgq lievpyrchq cerafvqrad clahlesdht elqlpadieh
     1261 evvaahmksi ipgtsedygl criacvfclr rfvstdslkk hvlgchtgdt facdtchqkl
     1321 psknsitrhk rkhhdlllse pgdsseeeqp nsedqpqtnh khsskkllll gkpkkkaada
     1381 aetgstetks plecgngdli qnllgiqdsk iidemlmnpa dsaarllgvk qv
//
LOCUS       XP_077502491             931 aa            linear   INV 25-NOV-2025
DEFINITION  epithelial splicing regulatory protein 2-like [Amblyomma
            americanum].
ACCESSION   XP_077502491
VERSION     XP_077502491.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646365.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..931
                     /product="epithelial splicing regulatory protein 2-like"
                     /calculated_mol_wt=99719
     Region          2..75
                     /region_name="RRM1_RBM12_like"
                     /note="RNA recognition motif 1 (RRM1) found in RNA-binding
                     protein RBM12, RBM12B and similar proteins; cd12510"
                     /db_xref="CDD:409932"
     Region          404..460
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          505..574
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          652..723
                     /region_name="RRM_hnRNPH_ESRPs_RBM12_like"
                     /note="RNA recognition motif (RRM) found in heterogeneous
                     nuclear ribonucleoprotein (hnRNP) H protein family,
                     epithelial splicing regulatory proteins (ESRPs),
                     Drosophila RNA-binding protein Fusilli, RNA-binding
                     protein 12 (RBM12) and similar proteins; cd12254"
                     /db_xref="CDD:409699"
     Region          <709..830
                     /region_name="PHA03264"
                     /note="envelope glycoprotein D; Provisional"
                     /db_xref="CDD:223029"
     Region          855..928
                     /region_name="RRM5_RBM12_like"
                     /note="RNA recognition motif 5 (RRM5) found in RNA-binding
                     protein RBM12, RBM12B and similar proteins; cd12515"
                     /db_xref="CDD:409937"
     Site            order(867..870,887,889)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:409937"
     CDS             1..931
                     /gene="LOC144113344"
                     /coded_by="XM_077646365.1:259..3054"
                     /db_xref="GeneID:144113344"
ORIGIN      
        1 msiiirlqnl pwaansldir ryfqglsipe ggvhivggek gdafiafgsd edarqamerd
       61 ggkikevrik lllssraemq riidharaqh tapppvkkee rrrpspddrs rrpqrdrspp
      121 rrrtsrrdrs hsrspsyrrd kdrsrssrsd errrsfdepg rvaepakvsn gqvperkgwd
      181 ekpafadpsk aatptfpigs lparqqnevg sapvnmacqq paalpnlaaf nlpavglmgn
      241 lsglaanmmg gimaggeamg gmagsvgdgv panvagnlst glaghmgggm avnmpgglpg
      301 nvsggvpsgm sgnmpghlgg mpagamgaga sggmaagmpg qmganmpgaf gmgdampnnv
      361 envgnmapgf vdrrmdgkgd rksdggapsg gardmprhae etccieirgl tgaptprdvk
      421 dlfrglrifg gcirvatsdn giksvvvrfa nkwdarealq gdfkvlcgdp iqicpfpedl
      481 feqtellvas pvgqqgrppr eadmvvmmkg lpyntseqdv lqffsglnvl divvehdrsg
      541 rvtgtafvef gdkrdfetam nmqrrkighr yielgvgsre vmyaarnges vgpdampmnr
      601 reeeppvmhg vgpergpela agpghgpgpm hgpgmgpahg pghslvppgh tcvsmlglps
      661 tvtdrdiadf fstqgvipra ihimlgangv ptghafaefg shadcerafl qdganlgphv
      721 ialktipyse vaqalgghpr pdgrpdgrpd grpdsrfdrr fegrfdgrph drgngrpdgg
      781 rpglmrpegr feppagppre rdgppakrpl lerssrpplf peprrgplfp erggpllrap
      841 rheeprmdgf gkpgcvvtat nipyragvdd iihffqgyel tkenvmrrfn drgqptgdar
      901 iafptprdaq ealskfnnrp mhgrsislgi m
//
LOCUS       XP_077502492             931 aa            linear   INV 25-NOV-2025
DEFINITION  epithelial splicing regulatory protein 2-like [Amblyomma
            americanum].
ACCESSION   XP_077502492
VERSION     XP_077502492.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646366.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..931
                     /product="epithelial splicing regulatory protein 2-like"
                     /calculated_mol_wt=99719
     Region          2..75
                     /region_name="RRM1_RBM12_like"
                     /note="RNA recognition motif 1 (RRM1) found in RNA-binding
                     protein RBM12, RBM12B and similar proteins; cd12510"
                     /db_xref="CDD:409932"
     Region          404..460
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          505..574
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          652..723
                     /region_name="RRM_hnRNPH_ESRPs_RBM12_like"
                     /note="RNA recognition motif (RRM) found in heterogeneous
                     nuclear ribonucleoprotein (hnRNP) H protein family,
                     epithelial splicing regulatory proteins (ESRPs),
                     Drosophila RNA-binding protein Fusilli, RNA-binding
                     protein 12 (RBM12) and similar proteins; cd12254"
                     /db_xref="CDD:409699"
     Region          <709..830
                     /region_name="PHA03264"
                     /note="envelope glycoprotein D; Provisional"
                     /db_xref="CDD:223029"
     Region          855..928
                     /region_name="RRM5_RBM12_like"
                     /note="RNA recognition motif 5 (RRM5) found in RNA-binding
                     protein RBM12, RBM12B and similar proteins; cd12515"
                     /db_xref="CDD:409937"
     Site            order(867..870,887,889)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:409937"
     CDS             1..931
                     /gene="LOC144113344"
                     /coded_by="XM_077646366.1:378..3173"
                     /db_xref="GeneID:144113344"
ORIGIN      
        1 msiiirlqnl pwaansldir ryfqglsipe ggvhivggek gdafiafgsd edarqamerd
       61 ggkikevrik lllssraemq riidharaqh tapppvkkee rrrpspddrs rrpqrdrspp
      121 rrrtsrrdrs hsrspsyrrd kdrsrssrsd errrsfdepg rvaepakvsn gqvperkgwd
      181 ekpafadpsk aatptfpigs lparqqnevg sapvnmacqq paalpnlaaf nlpavglmgn
      241 lsglaanmmg gimaggeamg gmagsvgdgv panvagnlst glaghmgggm avnmpgglpg
      301 nvsggvpsgm sgnmpghlgg mpagamgaga sggmaagmpg qmganmpgaf gmgdampnnv
      361 envgnmapgf vdrrmdgkgd rksdggapsg gardmprhae etccieirgl tgaptprdvk
      421 dlfrglrifg gcirvatsdn giksvvvrfa nkwdarealq gdfkvlcgdp iqicpfpedl
      481 feqtellvas pvgqqgrppr eadmvvmmkg lpyntseqdv lqffsglnvl divvehdrsg
      541 rvtgtafvef gdkrdfetam nmqrrkighr yielgvgsre vmyaarnges vgpdampmnr
      601 reeeppvmhg vgpergpela agpghgpgpm hgpgmgpahg pghslvppgh tcvsmlglps
      661 tvtdrdiadf fstqgvipra ihimlgangv ptghafaefg shadcerafl qdganlgphv
      721 ialktipyse vaqalgghpr pdgrpdgrpd grpdsrfdrr fegrfdgrph drgngrpdgg
      781 rpglmrpegr feppagppre rdgppakrpl lerssrpplf peprrgplfp erggpllrap
      841 rheeprmdgf gkpgcvvtat nipyragvdd iihffqgyel tkenvmrrfn drgqptgdar
      901 iafptprdaq ealskfnnrp mhgrsislgi m
//
LOCUS       XP_077502493             931 aa            linear   INV 25-NOV-2025
DEFINITION  epithelial splicing regulatory protein 2-like [Amblyomma
            americanum].
ACCESSION   XP_077502493
VERSION     XP_077502493.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646367.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..931
                     /product="epithelial splicing regulatory protein 2-like"
                     /calculated_mol_wt=99719
     Region          2..75
                     /region_name="RRM1_RBM12_like"
                     /note="RNA recognition motif 1 (RRM1) found in RNA-binding
                     protein RBM12, RBM12B and similar proteins; cd12510"
                     /db_xref="CDD:409932"
     Region          404..460
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          505..574
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          652..723
                     /region_name="RRM_hnRNPH_ESRPs_RBM12_like"
                     /note="RNA recognition motif (RRM) found in heterogeneous
                     nuclear ribonucleoprotein (hnRNP) H protein family,
                     epithelial splicing regulatory proteins (ESRPs),
                     Drosophila RNA-binding protein Fusilli, RNA-binding
                     protein 12 (RBM12) and similar proteins; cd12254"
                     /db_xref="CDD:409699"
     Region          <709..830
                     /region_name="PHA03264"
                     /note="envelope glycoprotein D; Provisional"
                     /db_xref="CDD:223029"
     Region          855..928
                     /region_name="RRM5_RBM12_like"
                     /note="RNA recognition motif 5 (RRM5) found in RNA-binding
                     protein RBM12, RBM12B and similar proteins; cd12515"
                     /db_xref="CDD:409937"
     Site            order(867..870,887,889)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:409937"
     CDS             1..931
                     /gene="LOC144113344"
                     /coded_by="XM_077646367.1:317..3112"
                     /db_xref="GeneID:144113344"
ORIGIN      
        1 msiiirlqnl pwaansldir ryfqglsipe ggvhivggek gdafiafgsd edarqamerd
       61 ggkikevrik lllssraemq riidharaqh tapppvkkee rrrpspddrs rrpqrdrspp
      121 rrrtsrrdrs hsrspsyrrd kdrsrssrsd errrsfdepg rvaepakvsn gqvperkgwd
      181 ekpafadpsk aatptfpigs lparqqnevg sapvnmacqq paalpnlaaf nlpavglmgn
      241 lsglaanmmg gimaggeamg gmagsvgdgv panvagnlst glaghmgggm avnmpgglpg
      301 nvsggvpsgm sgnmpghlgg mpagamgaga sggmaagmpg qmganmpgaf gmgdampnnv
      361 envgnmapgf vdrrmdgkgd rksdggapsg gardmprhae etccieirgl tgaptprdvk
      421 dlfrglrifg gcirvatsdn giksvvvrfa nkwdarealq gdfkvlcgdp iqicpfpedl
      481 feqtellvas pvgqqgrppr eadmvvmmkg lpyntseqdv lqffsglnvl divvehdrsg
      541 rvtgtafvef gdkrdfetam nmqrrkighr yielgvgsre vmyaarnges vgpdampmnr
      601 reeeppvmhg vgpergpela agpghgpgpm hgpgmgpahg pghslvppgh tcvsmlglps
      661 tvtdrdiadf fstqgvipra ihimlgangv ptghafaefg shadcerafl qdganlgphv
      721 ialktipyse vaqalgghpr pdgrpdgrpd grpdsrfdrr fegrfdgrph drgngrpdgg
      781 rpglmrpegr feppagppre rdgppakrpl lerssrpplf peprrgplfp erggpllrap
      841 rheeprmdgf gkpgcvvtat nipyragvdd iihffqgyel tkenvmrrfn drgqptgdar
      901 iafptprdaq ealskfnnrp mhgrsislgi m
//
LOCUS       XP_077502494            1482 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113346 [Amblyomma americanum].
ACCESSION   XP_077502494
VERSION     XP_077502494.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646368.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1482
                     /product="uncharacterized protein LOC144113346"
                     /calculated_mol_wt=167526
     Region          518..1078
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1482
                     /gene="LOC144113346"
                     /coded_by="XM_077646368.1:1576..6024"
                     /db_xref="GeneID:144113346"
ORIGIN      
        1 meppakpllp gpmpltsafc ardrqdncre malrihclds gwgtptasql rrllglqcav
       61 dqvvagdcge vevyespnwt anvtlklers pkdsfmhivl kwcdqyqngs nngaaagksg
      121 ppewwlasee leefcdpyst qiatehkdvp vnafalgcfl gyntfvahfd stytmpppps
      181 plssakssss hsgksmsvkr gkkgrgakss cpsdgtsssa ktacpnselt spegfkvtqc
      241 nahfehcsng lylyfvdvrk rsstrfrllv rysticriil rktsptctiv ylqllhppll
      301 ykvcagnsat sqcnregdyl pqfdlsdttp wdravefgeg pfkcgadvlg rsrvmrltls
      361 gprhwaplcn lilrtrknaq vfyaplrelq arelkavarp svqdfgvmya llsvwsesfq
      421 vtdelamdaq scretllela ahaeecpral eealfeiyfs lsqgkvfwfr salehlyfry
      481 rahrqridvf tgsviaqelp shmvkvrkvi ltptrrvymp pqlvcksrll qhcdpdyalr
      541 ivvrdddckl isfslgackd dflsavikpp lssglkiggr rfmflgcsts qlrnhgiwfy
      601 ahdnqgrtae qirdgigdls tipsvpkfma rlgqafsqsl glltvprqnt sveqdirhaa
      661 pgrssdgshr eyifsdgigr ishsllrkvh haleleegee pcaiqirygg ckgmllldpt
      721 lsgcrivfre smrkfhsdhs dlyvlktskp rvlylnrpmi tileqsgika evflmlqnki
      781 ldsfidsmmd pheaahvlra ycalrlpyke lagvgidltv epffralvra vnkkvlkelr
      841 tkarilvppn ygrtmfgvld etgtleygqv fvqysndmlr ykvndpatil egdvivtknp
      901 cmhpgdirkl eavnvpqlhh vrdcivfpqk gerphpdema gsdldgdeys vlwyedlifn
      961 nncnpmhyys dppkerkasi gvqdmvdffc qyikgdnigl ianahlvwad ildgginshr
     1021 crelarkcav nldfakcgdl kglqnsekpp mypdfmekld tkntycsrkv lgqlyrnckk
     1081 velsteclev veeslpdprl llegreqfmk eatsaykrya kkirallksy rieseseals
     1141 gavsklskym kendstdmam vlesqvehvv rqtreeffse qldeahenlk asawyqvtye
     1201 lqsseggiqs fpwvvsdvlm rivvntsscl pvpasrnsfs qrlgalllrv phpgggdqdd
     1261 aqhgdtqvlt nllrlmydwi dssreflfik daeelglykn imreacvkvs rslsrdmpph
     1321 klvimclrfa cawclkifqg gsdgeviske crrryrlghl alitlnrlsm sgnlaylcra
     1381 pegcpsteli riyinredev lfeilrryed ivteimtdws gvediqcdlk idrmddwflq
     1441 lmvtgsrwal erlkeivvyp sfrevlllaf erkknaiagt le
//
LOCUS       XP_077502495            1482 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113346 [Amblyomma americanum].
ACCESSION   XP_077502495
VERSION     XP_077502495.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646369.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1482
                     /product="uncharacterized protein LOC144113346"
                     /calculated_mol_wt=167526
     Region          518..1078
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1482
                     /gene="LOC144113346"
                     /coded_by="XM_077646369.1:1660..6108"
                     /db_xref="GeneID:144113346"
ORIGIN      
        1 meppakpllp gpmpltsafc ardrqdncre malrihclds gwgtptasql rrllglqcav
       61 dqvvagdcge vevyespnwt anvtlklers pkdsfmhivl kwcdqyqngs nngaaagksg
      121 ppewwlasee leefcdpyst qiatehkdvp vnafalgcfl gyntfvahfd stytmpppps
      181 plssakssss hsgksmsvkr gkkgrgakss cpsdgtsssa ktacpnselt spegfkvtqc
      241 nahfehcsng lylyfvdvrk rsstrfrllv rysticriil rktsptctiv ylqllhppll
      301 ykvcagnsat sqcnregdyl pqfdlsdttp wdravefgeg pfkcgadvlg rsrvmrltls
      361 gprhwaplcn lilrtrknaq vfyaplrelq arelkavarp svqdfgvmya llsvwsesfq
      421 vtdelamdaq scretllela ahaeecpral eealfeiyfs lsqgkvfwfr salehlyfry
      481 rahrqridvf tgsviaqelp shmvkvrkvi ltptrrvymp pqlvcksrll qhcdpdyalr
      541 ivvrdddckl isfslgackd dflsavikpp lssglkiggr rfmflgcsts qlrnhgiwfy
      601 ahdnqgrtae qirdgigdls tipsvpkfma rlgqafsqsl glltvprqnt sveqdirhaa
      661 pgrssdgshr eyifsdgigr ishsllrkvh haleleegee pcaiqirygg ckgmllldpt
      721 lsgcrivfre smrkfhsdhs dlyvlktskp rvlylnrpmi tileqsgika evflmlqnki
      781 ldsfidsmmd pheaahvlra ycalrlpyke lagvgidltv epffralvra vnkkvlkelr
      841 tkarilvppn ygrtmfgvld etgtleygqv fvqysndmlr ykvndpatil egdvivtknp
      901 cmhpgdirkl eavnvpqlhh vrdcivfpqk gerphpdema gsdldgdeys vlwyedlifn
      961 nncnpmhyys dppkerkasi gvqdmvdffc qyikgdnigl ianahlvwad ildgginshr
     1021 crelarkcav nldfakcgdl kglqnsekpp mypdfmekld tkntycsrkv lgqlyrnckk
     1081 velsteclev veeslpdprl llegreqfmk eatsaykrya kkirallksy rieseseals
     1141 gavsklskym kendstdmam vlesqvehvv rqtreeffse qldeahenlk asawyqvtye
     1201 lqsseggiqs fpwvvsdvlm rivvntsscl pvpasrnsfs qrlgalllrv phpgggdqdd
     1261 aqhgdtqvlt nllrlmydwi dssreflfik daeelglykn imreacvkvs rslsrdmpph
     1321 klvimclrfa cawclkifqg gsdgeviske crrryrlghl alitlnrlsm sgnlaylcra
     1381 pegcpsteli riyinredev lfeilrryed ivteimtdws gvediqcdlk idrmddwflq
     1441 lmvtgsrwal erlkeivvyp sfrevlllaf erkknaiagt le
//
LOCUS       XP_077502496            1482 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113346 [Amblyomma americanum].
ACCESSION   XP_077502496
VERSION     XP_077502496.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646370.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1482
                     /product="uncharacterized protein LOC144113346"
                     /calculated_mol_wt=167526
     Region          518..1078
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1482
                     /gene="LOC144113346"
                     /coded_by="XM_077646370.1:1435..5883"
                     /db_xref="GeneID:144113346"
ORIGIN      
        1 meppakpllp gpmpltsafc ardrqdncre malrihclds gwgtptasql rrllglqcav
       61 dqvvagdcge vevyespnwt anvtlklers pkdsfmhivl kwcdqyqngs nngaaagksg
      121 ppewwlasee leefcdpyst qiatehkdvp vnafalgcfl gyntfvahfd stytmpppps
      181 plssakssss hsgksmsvkr gkkgrgakss cpsdgtsssa ktacpnselt spegfkvtqc
      241 nahfehcsng lylyfvdvrk rsstrfrllv rysticriil rktsptctiv ylqllhppll
      301 ykvcagnsat sqcnregdyl pqfdlsdttp wdravefgeg pfkcgadvlg rsrvmrltls
      361 gprhwaplcn lilrtrknaq vfyaplrelq arelkavarp svqdfgvmya llsvwsesfq
      421 vtdelamdaq scretllela ahaeecpral eealfeiyfs lsqgkvfwfr salehlyfry
      481 rahrqridvf tgsviaqelp shmvkvrkvi ltptrrvymp pqlvcksrll qhcdpdyalr
      541 ivvrdddckl isfslgackd dflsavikpp lssglkiggr rfmflgcsts qlrnhgiwfy
      601 ahdnqgrtae qirdgigdls tipsvpkfma rlgqafsqsl glltvprqnt sveqdirhaa
      661 pgrssdgshr eyifsdgigr ishsllrkvh haleleegee pcaiqirygg ckgmllldpt
      721 lsgcrivfre smrkfhsdhs dlyvlktskp rvlylnrpmi tileqsgika evflmlqnki
      781 ldsfidsmmd pheaahvlra ycalrlpyke lagvgidltv epffralvra vnkkvlkelr
      841 tkarilvppn ygrtmfgvld etgtleygqv fvqysndmlr ykvndpatil egdvivtknp
      901 cmhpgdirkl eavnvpqlhh vrdcivfpqk gerphpdema gsdldgdeys vlwyedlifn
      961 nncnpmhyys dppkerkasi gvqdmvdffc qyikgdnigl ianahlvwad ildgginshr
     1021 crelarkcav nldfakcgdl kglqnsekpp mypdfmekld tkntycsrkv lgqlyrnckk
     1081 velsteclev veeslpdprl llegreqfmk eatsaykrya kkirallksy rieseseals
     1141 gavsklskym kendstdmam vlesqvehvv rqtreeffse qldeahenlk asawyqvtye
     1201 lqsseggiqs fpwvvsdvlm rivvntsscl pvpasrnsfs qrlgalllrv phpgggdqdd
     1261 aqhgdtqvlt nllrlmydwi dssreflfik daeelglykn imreacvkvs rslsrdmpph
     1321 klvimclrfa cawclkifqg gsdgeviske crrryrlghl alitlnrlsm sgnlaylcra
     1381 pegcpsteli riyinredev lfeilrryed ivteimtdws gvediqcdlk idrmddwflq
     1441 lmvtgsrwal erlkeivvyp sfrevlllaf erkknaiagt le
//
LOCUS       XP_077502497            1482 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113346 [Amblyomma americanum].
ACCESSION   XP_077502497
VERSION     XP_077502497.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646371.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1482
                     /product="uncharacterized protein LOC144113346"
                     /calculated_mol_wt=167526
     Region          518..1078
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1482
                     /gene="LOC144113346"
                     /coded_by="XM_077646371.1:1518..5966"
                     /db_xref="GeneID:144113346"
ORIGIN      
        1 meppakpllp gpmpltsafc ardrqdncre malrihclds gwgtptasql rrllglqcav
       61 dqvvagdcge vevyespnwt anvtlklers pkdsfmhivl kwcdqyqngs nngaaagksg
      121 ppewwlasee leefcdpyst qiatehkdvp vnafalgcfl gyntfvahfd stytmpppps
      181 plssakssss hsgksmsvkr gkkgrgakss cpsdgtsssa ktacpnselt spegfkvtqc
      241 nahfehcsng lylyfvdvrk rsstrfrllv rysticriil rktsptctiv ylqllhppll
      301 ykvcagnsat sqcnregdyl pqfdlsdttp wdravefgeg pfkcgadvlg rsrvmrltls
      361 gprhwaplcn lilrtrknaq vfyaplrelq arelkavarp svqdfgvmya llsvwsesfq
      421 vtdelamdaq scretllela ahaeecpral eealfeiyfs lsqgkvfwfr salehlyfry
      481 rahrqridvf tgsviaqelp shmvkvrkvi ltptrrvymp pqlvcksrll qhcdpdyalr
      541 ivvrdddckl isfslgackd dflsavikpp lssglkiggr rfmflgcsts qlrnhgiwfy
      601 ahdnqgrtae qirdgigdls tipsvpkfma rlgqafsqsl glltvprqnt sveqdirhaa
      661 pgrssdgshr eyifsdgigr ishsllrkvh haleleegee pcaiqirygg ckgmllldpt
      721 lsgcrivfre smrkfhsdhs dlyvlktskp rvlylnrpmi tileqsgika evflmlqnki
      781 ldsfidsmmd pheaahvlra ycalrlpyke lagvgidltv epffralvra vnkkvlkelr
      841 tkarilvppn ygrtmfgvld etgtleygqv fvqysndmlr ykvndpatil egdvivtknp
      901 cmhpgdirkl eavnvpqlhh vrdcivfpqk gerphpdema gsdldgdeys vlwyedlifn
      961 nncnpmhyys dppkerkasi gvqdmvdffc qyikgdnigl ianahlvwad ildgginshr
     1021 crelarkcav nldfakcgdl kglqnsekpp mypdfmekld tkntycsrkv lgqlyrnckk
     1081 velsteclev veeslpdprl llegreqfmk eatsaykrya kkirallksy rieseseals
     1141 gavsklskym kendstdmam vlesqvehvv rqtreeffse qldeahenlk asawyqvtye
     1201 lqsseggiqs fpwvvsdvlm rivvntsscl pvpasrnsfs qrlgalllrv phpgggdqdd
     1261 aqhgdtqvlt nllrlmydwi dssreflfik daeelglykn imreacvkvs rslsrdmpph
     1321 klvimclrfa cawclkifqg gsdgeviske crrryrlghl alitlnrlsm sgnlaylcra
     1381 pegcpsteli riyinredev lfeilrryed ivteimtdws gvediqcdlk idrmddwflq
     1441 lmvtgsrwal erlkeivvyp sfrevlllaf erkknaiagt le
//
LOCUS       XP_077502498            1482 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113346 [Amblyomma americanum].
ACCESSION   XP_077502498
VERSION     XP_077502498.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646372.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1482
                     /product="uncharacterized protein LOC144113346"
                     /calculated_mol_wt=167526
     Region          518..1078
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1482
                     /gene="LOC144113346"
                     /coded_by="XM_077646372.1:1490..5938"
                     /db_xref="GeneID:144113346"
ORIGIN      
        1 meppakpllp gpmpltsafc ardrqdncre malrihclds gwgtptasql rrllglqcav
       61 dqvvagdcge vevyespnwt anvtlklers pkdsfmhivl kwcdqyqngs nngaaagksg
      121 ppewwlasee leefcdpyst qiatehkdvp vnafalgcfl gyntfvahfd stytmpppps
      181 plssakssss hsgksmsvkr gkkgrgakss cpsdgtsssa ktacpnselt spegfkvtqc
      241 nahfehcsng lylyfvdvrk rsstrfrllv rysticriil rktsptctiv ylqllhppll
      301 ykvcagnsat sqcnregdyl pqfdlsdttp wdravefgeg pfkcgadvlg rsrvmrltls
      361 gprhwaplcn lilrtrknaq vfyaplrelq arelkavarp svqdfgvmya llsvwsesfq
      421 vtdelamdaq scretllela ahaeecpral eealfeiyfs lsqgkvfwfr salehlyfry
      481 rahrqridvf tgsviaqelp shmvkvrkvi ltptrrvymp pqlvcksrll qhcdpdyalr
      541 ivvrdddckl isfslgackd dflsavikpp lssglkiggr rfmflgcsts qlrnhgiwfy
      601 ahdnqgrtae qirdgigdls tipsvpkfma rlgqafsqsl glltvprqnt sveqdirhaa
      661 pgrssdgshr eyifsdgigr ishsllrkvh haleleegee pcaiqirygg ckgmllldpt
      721 lsgcrivfre smrkfhsdhs dlyvlktskp rvlylnrpmi tileqsgika evflmlqnki
      781 ldsfidsmmd pheaahvlra ycalrlpyke lagvgidltv epffralvra vnkkvlkelr
      841 tkarilvppn ygrtmfgvld etgtleygqv fvqysndmlr ykvndpatil egdvivtknp
      901 cmhpgdirkl eavnvpqlhh vrdcivfpqk gerphpdema gsdldgdeys vlwyedlifn
      961 nncnpmhyys dppkerkasi gvqdmvdffc qyikgdnigl ianahlvwad ildgginshr
     1021 crelarkcav nldfakcgdl kglqnsekpp mypdfmekld tkntycsrkv lgqlyrnckk
     1081 velsteclev veeslpdprl llegreqfmk eatsaykrya kkirallksy rieseseals
     1141 gavsklskym kendstdmam vlesqvehvv rqtreeffse qldeahenlk asawyqvtye
     1201 lqsseggiqs fpwvvsdvlm rivvntsscl pvpasrnsfs qrlgalllrv phpgggdqdd
     1261 aqhgdtqvlt nllrlmydwi dssreflfik daeelglykn imreacvkvs rslsrdmpph
     1321 klvimclrfa cawclkifqg gsdgeviske crrryrlghl alitlnrlsm sgnlaylcra
     1381 pegcpsteli riyinredev lfeilrryed ivteimtdws gvediqcdlk idrmddwflq
     1441 lmvtgsrwal erlkeivvyp sfrevlllaf erkknaiagt le
//
LOCUS       XP_077502499            1482 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113346 [Amblyomma americanum].
ACCESSION   XP_077502499
VERSION     XP_077502499.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646373.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1482
                     /product="uncharacterized protein LOC144113346"
                     /calculated_mol_wt=167526
     Region          518..1078
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1482
                     /gene="LOC144113346"
                     /coded_by="XM_077646373.1:1567..6015"
                     /db_xref="GeneID:144113346"
ORIGIN      
        1 meppakpllp gpmpltsafc ardrqdncre malrihclds gwgtptasql rrllglqcav
       61 dqvvagdcge vevyespnwt anvtlklers pkdsfmhivl kwcdqyqngs nngaaagksg
      121 ppewwlasee leefcdpyst qiatehkdvp vnafalgcfl gyntfvahfd stytmpppps
      181 plssakssss hsgksmsvkr gkkgrgakss cpsdgtsssa ktacpnselt spegfkvtqc
      241 nahfehcsng lylyfvdvrk rsstrfrllv rysticriil rktsptctiv ylqllhppll
      301 ykvcagnsat sqcnregdyl pqfdlsdttp wdravefgeg pfkcgadvlg rsrvmrltls
      361 gprhwaplcn lilrtrknaq vfyaplrelq arelkavarp svqdfgvmya llsvwsesfq
      421 vtdelamdaq scretllela ahaeecpral eealfeiyfs lsqgkvfwfr salehlyfry
      481 rahrqridvf tgsviaqelp shmvkvrkvi ltptrrvymp pqlvcksrll qhcdpdyalr
      541 ivvrdddckl isfslgackd dflsavikpp lssglkiggr rfmflgcsts qlrnhgiwfy
      601 ahdnqgrtae qirdgigdls tipsvpkfma rlgqafsqsl glltvprqnt sveqdirhaa
      661 pgrssdgshr eyifsdgigr ishsllrkvh haleleegee pcaiqirygg ckgmllldpt
      721 lsgcrivfre smrkfhsdhs dlyvlktskp rvlylnrpmi tileqsgika evflmlqnki
      781 ldsfidsmmd pheaahvlra ycalrlpyke lagvgidltv epffralvra vnkkvlkelr
      841 tkarilvppn ygrtmfgvld etgtleygqv fvqysndmlr ykvndpatil egdvivtknp
      901 cmhpgdirkl eavnvpqlhh vrdcivfpqk gerphpdema gsdldgdeys vlwyedlifn
      961 nncnpmhyys dppkerkasi gvqdmvdffc qyikgdnigl ianahlvwad ildgginshr
     1021 crelarkcav nldfakcgdl kglqnsekpp mypdfmekld tkntycsrkv lgqlyrnckk
     1081 velsteclev veeslpdprl llegreqfmk eatsaykrya kkirallksy rieseseals
     1141 gavsklskym kendstdmam vlesqvehvv rqtreeffse qldeahenlk asawyqvtye
     1201 lqsseggiqs fpwvvsdvlm rivvntsscl pvpasrnsfs qrlgalllrv phpgggdqdd
     1261 aqhgdtqvlt nllrlmydwi dssreflfik daeelglykn imreacvkvs rslsrdmpph
     1321 klvimclrfa cawclkifqg gsdgeviske crrryrlghl alitlnrlsm sgnlaylcra
     1381 pegcpsteli riyinredev lfeilrryed ivteimtdws gvediqcdlk idrmddwflq
     1441 lmvtgsrwal erlkeivvyp sfrevlllaf erkknaiagt le
//
LOCUS       XP_077502500            1482 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113346 [Amblyomma americanum].
ACCESSION   XP_077502500
VERSION     XP_077502500.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646374.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1482
                     /product="uncharacterized protein LOC144113346"
                     /calculated_mol_wt=167526
     Region          518..1078
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1482
                     /gene="LOC144113346"
                     /coded_by="XM_077646374.1:1570..6018"
                     /db_xref="GeneID:144113346"
ORIGIN      
        1 meppakpllp gpmpltsafc ardrqdncre malrihclds gwgtptasql rrllglqcav
       61 dqvvagdcge vevyespnwt anvtlklers pkdsfmhivl kwcdqyqngs nngaaagksg
      121 ppewwlasee leefcdpyst qiatehkdvp vnafalgcfl gyntfvahfd stytmpppps
      181 plssakssss hsgksmsvkr gkkgrgakss cpsdgtsssa ktacpnselt spegfkvtqc
      241 nahfehcsng lylyfvdvrk rsstrfrllv rysticriil rktsptctiv ylqllhppll
      301 ykvcagnsat sqcnregdyl pqfdlsdttp wdravefgeg pfkcgadvlg rsrvmrltls
      361 gprhwaplcn lilrtrknaq vfyaplrelq arelkavarp svqdfgvmya llsvwsesfq
      421 vtdelamdaq scretllela ahaeecpral eealfeiyfs lsqgkvfwfr salehlyfry
      481 rahrqridvf tgsviaqelp shmvkvrkvi ltptrrvymp pqlvcksrll qhcdpdyalr
      541 ivvrdddckl isfslgackd dflsavikpp lssglkiggr rfmflgcsts qlrnhgiwfy
      601 ahdnqgrtae qirdgigdls tipsvpkfma rlgqafsqsl glltvprqnt sveqdirhaa
      661 pgrssdgshr eyifsdgigr ishsllrkvh haleleegee pcaiqirygg ckgmllldpt
      721 lsgcrivfre smrkfhsdhs dlyvlktskp rvlylnrpmi tileqsgika evflmlqnki
      781 ldsfidsmmd pheaahvlra ycalrlpyke lagvgidltv epffralvra vnkkvlkelr
      841 tkarilvppn ygrtmfgvld etgtleygqv fvqysndmlr ykvndpatil egdvivtknp
      901 cmhpgdirkl eavnvpqlhh vrdcivfpqk gerphpdema gsdldgdeys vlwyedlifn
      961 nncnpmhyys dppkerkasi gvqdmvdffc qyikgdnigl ianahlvwad ildgginshr
     1021 crelarkcav nldfakcgdl kglqnsekpp mypdfmekld tkntycsrkv lgqlyrnckk
     1081 velsteclev veeslpdprl llegreqfmk eatsaykrya kkirallksy rieseseals
     1141 gavsklskym kendstdmam vlesqvehvv rqtreeffse qldeahenlk asawyqvtye
     1201 lqsseggiqs fpwvvsdvlm rivvntsscl pvpasrnsfs qrlgalllrv phpgggdqdd
     1261 aqhgdtqvlt nllrlmydwi dssreflfik daeelglykn imreacvkvs rslsrdmpph
     1321 klvimclrfa cawclkifqg gsdgeviske crrryrlghl alitlnrlsm sgnlaylcra
     1381 pegcpsteli riyinredev lfeilrryed ivteimtdws gvediqcdlk idrmddwflq
     1441 lmvtgsrwal erlkeivvyp sfrevlllaf erkknaiagt le
//
LOCUS       XP_077502502            1482 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113346 [Amblyomma americanum].
ACCESSION   XP_077502502
VERSION     XP_077502502.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646376.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1482
                     /product="uncharacterized protein LOC144113346"
                     /calculated_mol_wt=167526
     Region          518..1078
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1482
                     /gene="LOC144113346"
                     /coded_by="XM_077646376.1:1538..5986"
                     /db_xref="GeneID:144113346"
ORIGIN      
        1 meppakpllp gpmpltsafc ardrqdncre malrihclds gwgtptasql rrllglqcav
       61 dqvvagdcge vevyespnwt anvtlklers pkdsfmhivl kwcdqyqngs nngaaagksg
      121 ppewwlasee leefcdpyst qiatehkdvp vnafalgcfl gyntfvahfd stytmpppps
      181 plssakssss hsgksmsvkr gkkgrgakss cpsdgtsssa ktacpnselt spegfkvtqc
      241 nahfehcsng lylyfvdvrk rsstrfrllv rysticriil rktsptctiv ylqllhppll
      301 ykvcagnsat sqcnregdyl pqfdlsdttp wdravefgeg pfkcgadvlg rsrvmrltls
      361 gprhwaplcn lilrtrknaq vfyaplrelq arelkavarp svqdfgvmya llsvwsesfq
      421 vtdelamdaq scretllela ahaeecpral eealfeiyfs lsqgkvfwfr salehlyfry
      481 rahrqridvf tgsviaqelp shmvkvrkvi ltptrrvymp pqlvcksrll qhcdpdyalr
      541 ivvrdddckl isfslgackd dflsavikpp lssglkiggr rfmflgcsts qlrnhgiwfy
      601 ahdnqgrtae qirdgigdls tipsvpkfma rlgqafsqsl glltvprqnt sveqdirhaa
      661 pgrssdgshr eyifsdgigr ishsllrkvh haleleegee pcaiqirygg ckgmllldpt
      721 lsgcrivfre smrkfhsdhs dlyvlktskp rvlylnrpmi tileqsgika evflmlqnki
      781 ldsfidsmmd pheaahvlra ycalrlpyke lagvgidltv epffralvra vnkkvlkelr
      841 tkarilvppn ygrtmfgvld etgtleygqv fvqysndmlr ykvndpatil egdvivtknp
      901 cmhpgdirkl eavnvpqlhh vrdcivfpqk gerphpdema gsdldgdeys vlwyedlifn
      961 nncnpmhyys dppkerkasi gvqdmvdffc qyikgdnigl ianahlvwad ildgginshr
     1021 crelarkcav nldfakcgdl kglqnsekpp mypdfmekld tkntycsrkv lgqlyrnckk
     1081 velsteclev veeslpdprl llegreqfmk eatsaykrya kkirallksy rieseseals
     1141 gavsklskym kendstdmam vlesqvehvv rqtreeffse qldeahenlk asawyqvtye
     1201 lqsseggiqs fpwvvsdvlm rivvntsscl pvpasrnsfs qrlgalllrv phpgggdqdd
     1261 aqhgdtqvlt nllrlmydwi dssreflfik daeelglykn imreacvkvs rslsrdmpph
     1321 klvimclrfa cawclkifqg gsdgeviske crrryrlghl alitlnrlsm sgnlaylcra
     1381 pegcpsteli riyinredev lfeilrryed ivteimtdws gvediqcdlk idrmddwflq
     1441 lmvtgsrwal erlkeivvyp sfrevlllaf erkknaiagt le
//
LOCUS       XP_077502503            1482 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113346 [Amblyomma americanum].
ACCESSION   XP_077502503
VERSION     XP_077502503.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646377.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1482
                     /product="uncharacterized protein LOC144113346"
                     /calculated_mol_wt=167526
     Region          518..1078
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1482
                     /gene="LOC144113346"
                     /coded_by="XM_077646377.1:1560..6008"
                     /db_xref="GeneID:144113346"
ORIGIN      
        1 meppakpllp gpmpltsafc ardrqdncre malrihclds gwgtptasql rrllglqcav
       61 dqvvagdcge vevyespnwt anvtlklers pkdsfmhivl kwcdqyqngs nngaaagksg
      121 ppewwlasee leefcdpyst qiatehkdvp vnafalgcfl gyntfvahfd stytmpppps
      181 plssakssss hsgksmsvkr gkkgrgakss cpsdgtsssa ktacpnselt spegfkvtqc
      241 nahfehcsng lylyfvdvrk rsstrfrllv rysticriil rktsptctiv ylqllhppll
      301 ykvcagnsat sqcnregdyl pqfdlsdttp wdravefgeg pfkcgadvlg rsrvmrltls
      361 gprhwaplcn lilrtrknaq vfyaplrelq arelkavarp svqdfgvmya llsvwsesfq
      421 vtdelamdaq scretllela ahaeecpral eealfeiyfs lsqgkvfwfr salehlyfry
      481 rahrqridvf tgsviaqelp shmvkvrkvi ltptrrvymp pqlvcksrll qhcdpdyalr
      541 ivvrdddckl isfslgackd dflsavikpp lssglkiggr rfmflgcsts qlrnhgiwfy
      601 ahdnqgrtae qirdgigdls tipsvpkfma rlgqafsqsl glltvprqnt sveqdirhaa
      661 pgrssdgshr eyifsdgigr ishsllrkvh haleleegee pcaiqirygg ckgmllldpt
      721 lsgcrivfre smrkfhsdhs dlyvlktskp rvlylnrpmi tileqsgika evflmlqnki
      781 ldsfidsmmd pheaahvlra ycalrlpyke lagvgidltv epffralvra vnkkvlkelr
      841 tkarilvppn ygrtmfgvld etgtleygqv fvqysndmlr ykvndpatil egdvivtknp
      901 cmhpgdirkl eavnvpqlhh vrdcivfpqk gerphpdema gsdldgdeys vlwyedlifn
      961 nncnpmhyys dppkerkasi gvqdmvdffc qyikgdnigl ianahlvwad ildgginshr
     1021 crelarkcav nldfakcgdl kglqnsekpp mypdfmekld tkntycsrkv lgqlyrnckk
     1081 velsteclev veeslpdprl llegreqfmk eatsaykrya kkirallksy rieseseals
     1141 gavsklskym kendstdmam vlesqvehvv rqtreeffse qldeahenlk asawyqvtye
     1201 lqsseggiqs fpwvvsdvlm rivvntsscl pvpasrnsfs qrlgalllrv phpgggdqdd
     1261 aqhgdtqvlt nllrlmydwi dssreflfik daeelglykn imreacvkvs rslsrdmpph
     1321 klvimclrfa cawclkifqg gsdgeviske crrryrlghl alitlnrlsm sgnlaylcra
     1381 pegcpsteli riyinredev lfeilrryed ivteimtdws gvediqcdlk idrmddwflq
     1441 lmvtgsrwal erlkeivvyp sfrevlllaf erkknaiagt le
//
LOCUS       XP_077502504            1482 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113346 [Amblyomma americanum].
ACCESSION   XP_077502504
VERSION     XP_077502504.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646378.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1482
                     /product="uncharacterized protein LOC144113346"
                     /calculated_mol_wt=167526
     Region          518..1078
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1482
                     /gene="LOC144113346"
                     /coded_by="XM_077646378.1:1484..5932"
                     /db_xref="GeneID:144113346"
ORIGIN      
        1 meppakpllp gpmpltsafc ardrqdncre malrihclds gwgtptasql rrllglqcav
       61 dqvvagdcge vevyespnwt anvtlklers pkdsfmhivl kwcdqyqngs nngaaagksg
      121 ppewwlasee leefcdpyst qiatehkdvp vnafalgcfl gyntfvahfd stytmpppps
      181 plssakssss hsgksmsvkr gkkgrgakss cpsdgtsssa ktacpnselt spegfkvtqc
      241 nahfehcsng lylyfvdvrk rsstrfrllv rysticriil rktsptctiv ylqllhppll
      301 ykvcagnsat sqcnregdyl pqfdlsdttp wdravefgeg pfkcgadvlg rsrvmrltls
      361 gprhwaplcn lilrtrknaq vfyaplrelq arelkavarp svqdfgvmya llsvwsesfq
      421 vtdelamdaq scretllela ahaeecpral eealfeiyfs lsqgkvfwfr salehlyfry
      481 rahrqridvf tgsviaqelp shmvkvrkvi ltptrrvymp pqlvcksrll qhcdpdyalr
      541 ivvrdddckl isfslgackd dflsavikpp lssglkiggr rfmflgcsts qlrnhgiwfy
      601 ahdnqgrtae qirdgigdls tipsvpkfma rlgqafsqsl glltvprqnt sveqdirhaa
      661 pgrssdgshr eyifsdgigr ishsllrkvh haleleegee pcaiqirygg ckgmllldpt
      721 lsgcrivfre smrkfhsdhs dlyvlktskp rvlylnrpmi tileqsgika evflmlqnki
      781 ldsfidsmmd pheaahvlra ycalrlpyke lagvgidltv epffralvra vnkkvlkelr
      841 tkarilvppn ygrtmfgvld etgtleygqv fvqysndmlr ykvndpatil egdvivtknp
      901 cmhpgdirkl eavnvpqlhh vrdcivfpqk gerphpdema gsdldgdeys vlwyedlifn
      961 nncnpmhyys dppkerkasi gvqdmvdffc qyikgdnigl ianahlvwad ildgginshr
     1021 crelarkcav nldfakcgdl kglqnsekpp mypdfmekld tkntycsrkv lgqlyrnckk
     1081 velsteclev veeslpdprl llegreqfmk eatsaykrya kkirallksy rieseseals
     1141 gavsklskym kendstdmam vlesqvehvv rqtreeffse qldeahenlk asawyqvtye
     1201 lqsseggiqs fpwvvsdvlm rivvntsscl pvpasrnsfs qrlgalllrv phpgggdqdd
     1261 aqhgdtqvlt nllrlmydwi dssreflfik daeelglykn imreacvkvs rslsrdmpph
     1321 klvimclrfa cawclkifqg gsdgeviske crrryrlghl alitlnrlsm sgnlaylcra
     1381 pegcpsteli riyinredev lfeilrryed ivteimtdws gvediqcdlk idrmddwflq
     1441 lmvtgsrwal erlkeivvyp sfrevlllaf erkknaiagt le
//
LOCUS       XP_077502505            1482 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113346 [Amblyomma americanum].
ACCESSION   XP_077502505
VERSION     XP_077502505.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646379.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1482
                     /product="uncharacterized protein LOC144113346"
                     /calculated_mol_wt=167526
     Region          518..1078
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1482
                     /gene="LOC144113346"
                     /coded_by="XM_077646379.1:1343..5791"
                     /db_xref="GeneID:144113346"
ORIGIN      
        1 meppakpllp gpmpltsafc ardrqdncre malrihclds gwgtptasql rrllglqcav
       61 dqvvagdcge vevyespnwt anvtlklers pkdsfmhivl kwcdqyqngs nngaaagksg
      121 ppewwlasee leefcdpyst qiatehkdvp vnafalgcfl gyntfvahfd stytmpppps
      181 plssakssss hsgksmsvkr gkkgrgakss cpsdgtsssa ktacpnselt spegfkvtqc
      241 nahfehcsng lylyfvdvrk rsstrfrllv rysticriil rktsptctiv ylqllhppll
      301 ykvcagnsat sqcnregdyl pqfdlsdttp wdravefgeg pfkcgadvlg rsrvmrltls
      361 gprhwaplcn lilrtrknaq vfyaplrelq arelkavarp svqdfgvmya llsvwsesfq
      421 vtdelamdaq scretllela ahaeecpral eealfeiyfs lsqgkvfwfr salehlyfry
      481 rahrqridvf tgsviaqelp shmvkvrkvi ltptrrvymp pqlvcksrll qhcdpdyalr
      541 ivvrdddckl isfslgackd dflsavikpp lssglkiggr rfmflgcsts qlrnhgiwfy
      601 ahdnqgrtae qirdgigdls tipsvpkfma rlgqafsqsl glltvprqnt sveqdirhaa
      661 pgrssdgshr eyifsdgigr ishsllrkvh haleleegee pcaiqirygg ckgmllldpt
      721 lsgcrivfre smrkfhsdhs dlyvlktskp rvlylnrpmi tileqsgika evflmlqnki
      781 ldsfidsmmd pheaahvlra ycalrlpyke lagvgidltv epffralvra vnkkvlkelr
      841 tkarilvppn ygrtmfgvld etgtleygqv fvqysndmlr ykvndpatil egdvivtknp
      901 cmhpgdirkl eavnvpqlhh vrdcivfpqk gerphpdema gsdldgdeys vlwyedlifn
      961 nncnpmhyys dppkerkasi gvqdmvdffc qyikgdnigl ianahlvwad ildgginshr
     1021 crelarkcav nldfakcgdl kglqnsekpp mypdfmekld tkntycsrkv lgqlyrnckk
     1081 velsteclev veeslpdprl llegreqfmk eatsaykrya kkirallksy rieseseals
     1141 gavsklskym kendstdmam vlesqvehvv rqtreeffse qldeahenlk asawyqvtye
     1201 lqsseggiqs fpwvvsdvlm rivvntsscl pvpasrnsfs qrlgalllrv phpgggdqdd
     1261 aqhgdtqvlt nllrlmydwi dssreflfik daeelglykn imreacvkvs rslsrdmpph
     1321 klvimclrfa cawclkifqg gsdgeviske crrryrlghl alitlnrlsm sgnlaylcra
     1381 pegcpsteli riyinredev lfeilrryed ivteimtdws gvediqcdlk idrmddwflq
     1441 lmvtgsrwal erlkeivvyp sfrevlllaf erkknaiagt le
//
LOCUS       XP_077502506            1482 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113346 [Amblyomma americanum].
ACCESSION   XP_077502506
VERSION     XP_077502506.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646380.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1482
                     /product="uncharacterized protein LOC144113346"
                     /calculated_mol_wt=167526
     Region          518..1078
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1482
                     /gene="LOC144113346"
                     /coded_by="XM_077646380.1:1530..5978"
                     /db_xref="GeneID:144113346"
ORIGIN      
        1 meppakpllp gpmpltsafc ardrqdncre malrihclds gwgtptasql rrllglqcav
       61 dqvvagdcge vevyespnwt anvtlklers pkdsfmhivl kwcdqyqngs nngaaagksg
      121 ppewwlasee leefcdpyst qiatehkdvp vnafalgcfl gyntfvahfd stytmpppps
      181 plssakssss hsgksmsvkr gkkgrgakss cpsdgtsssa ktacpnselt spegfkvtqc
      241 nahfehcsng lylyfvdvrk rsstrfrllv rysticriil rktsptctiv ylqllhppll
      301 ykvcagnsat sqcnregdyl pqfdlsdttp wdravefgeg pfkcgadvlg rsrvmrltls
      361 gprhwaplcn lilrtrknaq vfyaplrelq arelkavarp svqdfgvmya llsvwsesfq
      421 vtdelamdaq scretllela ahaeecpral eealfeiyfs lsqgkvfwfr salehlyfry
      481 rahrqridvf tgsviaqelp shmvkvrkvi ltptrrvymp pqlvcksrll qhcdpdyalr
      541 ivvrdddckl isfslgackd dflsavikpp lssglkiggr rfmflgcsts qlrnhgiwfy
      601 ahdnqgrtae qirdgigdls tipsvpkfma rlgqafsqsl glltvprqnt sveqdirhaa
      661 pgrssdgshr eyifsdgigr ishsllrkvh haleleegee pcaiqirygg ckgmllldpt
      721 lsgcrivfre smrkfhsdhs dlyvlktskp rvlylnrpmi tileqsgika evflmlqnki
      781 ldsfidsmmd pheaahvlra ycalrlpyke lagvgidltv epffralvra vnkkvlkelr
      841 tkarilvppn ygrtmfgvld etgtleygqv fvqysndmlr ykvndpatil egdvivtknp
      901 cmhpgdirkl eavnvpqlhh vrdcivfpqk gerphpdema gsdldgdeys vlwyedlifn
      961 nncnpmhyys dppkerkasi gvqdmvdffc qyikgdnigl ianahlvwad ildgginshr
     1021 crelarkcav nldfakcgdl kglqnsekpp mypdfmekld tkntycsrkv lgqlyrnckk
     1081 velsteclev veeslpdprl llegreqfmk eatsaykrya kkirallksy rieseseals
     1141 gavsklskym kendstdmam vlesqvehvv rqtreeffse qldeahenlk asawyqvtye
     1201 lqsseggiqs fpwvvsdvlm rivvntsscl pvpasrnsfs qrlgalllrv phpgggdqdd
     1261 aqhgdtqvlt nllrlmydwi dssreflfik daeelglykn imreacvkvs rslsrdmpph
     1321 klvimclrfa cawclkifqg gsdgeviske crrryrlghl alitlnrlsm sgnlaylcra
     1381 pegcpsteli riyinredev lfeilrryed ivteimtdws gvediqcdlk idrmddwflq
     1441 lmvtgsrwal erlkeivvyp sfrevlllaf erkknaiagt le
//
LOCUS       XP_077502507            1482 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113346 [Amblyomma americanum].
ACCESSION   XP_077502507
VERSION     XP_077502507.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646381.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1482
                     /product="uncharacterized protein LOC144113346"
                     /calculated_mol_wt=167526
     Region          518..1078
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1482
                     /gene="LOC144113346"
                     /coded_by="XM_077646381.1:1475..5923"
                     /db_xref="GeneID:144113346"
ORIGIN      
        1 meppakpllp gpmpltsafc ardrqdncre malrihclds gwgtptasql rrllglqcav
       61 dqvvagdcge vevyespnwt anvtlklers pkdsfmhivl kwcdqyqngs nngaaagksg
      121 ppewwlasee leefcdpyst qiatehkdvp vnafalgcfl gyntfvahfd stytmpppps
      181 plssakssss hsgksmsvkr gkkgrgakss cpsdgtsssa ktacpnselt spegfkvtqc
      241 nahfehcsng lylyfvdvrk rsstrfrllv rysticriil rktsptctiv ylqllhppll
      301 ykvcagnsat sqcnregdyl pqfdlsdttp wdravefgeg pfkcgadvlg rsrvmrltls
      361 gprhwaplcn lilrtrknaq vfyaplrelq arelkavarp svqdfgvmya llsvwsesfq
      421 vtdelamdaq scretllela ahaeecpral eealfeiyfs lsqgkvfwfr salehlyfry
      481 rahrqridvf tgsviaqelp shmvkvrkvi ltptrrvymp pqlvcksrll qhcdpdyalr
      541 ivvrdddckl isfslgackd dflsavikpp lssglkiggr rfmflgcsts qlrnhgiwfy
      601 ahdnqgrtae qirdgigdls tipsvpkfma rlgqafsqsl glltvprqnt sveqdirhaa
      661 pgrssdgshr eyifsdgigr ishsllrkvh haleleegee pcaiqirygg ckgmllldpt
      721 lsgcrivfre smrkfhsdhs dlyvlktskp rvlylnrpmi tileqsgika evflmlqnki
      781 ldsfidsmmd pheaahvlra ycalrlpyke lagvgidltv epffralvra vnkkvlkelr
      841 tkarilvppn ygrtmfgvld etgtleygqv fvqysndmlr ykvndpatil egdvivtknp
      901 cmhpgdirkl eavnvpqlhh vrdcivfpqk gerphpdema gsdldgdeys vlwyedlifn
      961 nncnpmhyys dppkerkasi gvqdmvdffc qyikgdnigl ianahlvwad ildgginshr
     1021 crelarkcav nldfakcgdl kglqnsekpp mypdfmekld tkntycsrkv lgqlyrnckk
     1081 velsteclev veeslpdprl llegreqfmk eatsaykrya kkirallksy rieseseals
     1141 gavsklskym kendstdmam vlesqvehvv rqtreeffse qldeahenlk asawyqvtye
     1201 lqsseggiqs fpwvvsdvlm rivvntsscl pvpasrnsfs qrlgalllrv phpgggdqdd
     1261 aqhgdtqvlt nllrlmydwi dssreflfik daeelglykn imreacvkvs rslsrdmpph
     1321 klvimclrfa cawclkifqg gsdgeviske crrryrlghl alitlnrlsm sgnlaylcra
     1381 pegcpsteli riyinredev lfeilrryed ivteimtdws gvediqcdlk idrmddwflq
     1441 lmvtgsrwal erlkeivvyp sfrevlllaf erkknaiagt le
//
LOCUS       XP_077502508            1482 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113346 [Amblyomma americanum].
ACCESSION   XP_077502508
VERSION     XP_077502508.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646382.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1482
                     /product="uncharacterized protein LOC144113346"
                     /calculated_mol_wt=167526
     Region          518..1078
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1482
                     /gene="LOC144113346"
                     /coded_by="XM_077646382.1:2049..6497"
                     /db_xref="GeneID:144113346"
ORIGIN      
        1 meppakpllp gpmpltsafc ardrqdncre malrihclds gwgtptasql rrllglqcav
       61 dqvvagdcge vevyespnwt anvtlklers pkdsfmhivl kwcdqyqngs nngaaagksg
      121 ppewwlasee leefcdpyst qiatehkdvp vnafalgcfl gyntfvahfd stytmpppps
      181 plssakssss hsgksmsvkr gkkgrgakss cpsdgtsssa ktacpnselt spegfkvtqc
      241 nahfehcsng lylyfvdvrk rsstrfrllv rysticriil rktsptctiv ylqllhppll
      301 ykvcagnsat sqcnregdyl pqfdlsdttp wdravefgeg pfkcgadvlg rsrvmrltls
      361 gprhwaplcn lilrtrknaq vfyaplrelq arelkavarp svqdfgvmya llsvwsesfq
      421 vtdelamdaq scretllela ahaeecpral eealfeiyfs lsqgkvfwfr salehlyfry
      481 rahrqridvf tgsviaqelp shmvkvrkvi ltptrrvymp pqlvcksrll qhcdpdyalr
      541 ivvrdddckl isfslgackd dflsavikpp lssglkiggr rfmflgcsts qlrnhgiwfy
      601 ahdnqgrtae qirdgigdls tipsvpkfma rlgqafsqsl glltvprqnt sveqdirhaa
      661 pgrssdgshr eyifsdgigr ishsllrkvh haleleegee pcaiqirygg ckgmllldpt
      721 lsgcrivfre smrkfhsdhs dlyvlktskp rvlylnrpmi tileqsgika evflmlqnki
      781 ldsfidsmmd pheaahvlra ycalrlpyke lagvgidltv epffralvra vnkkvlkelr
      841 tkarilvppn ygrtmfgvld etgtleygqv fvqysndmlr ykvndpatil egdvivtknp
      901 cmhpgdirkl eavnvpqlhh vrdcivfpqk gerphpdema gsdldgdeys vlwyedlifn
      961 nncnpmhyys dppkerkasi gvqdmvdffc qyikgdnigl ianahlvwad ildgginshr
     1021 crelarkcav nldfakcgdl kglqnsekpp mypdfmekld tkntycsrkv lgqlyrnckk
     1081 velsteclev veeslpdprl llegreqfmk eatsaykrya kkirallksy rieseseals
     1141 gavsklskym kendstdmam vlesqvehvv rqtreeffse qldeahenlk asawyqvtye
     1201 lqsseggiqs fpwvvsdvlm rivvntsscl pvpasrnsfs qrlgalllrv phpgggdqdd
     1261 aqhgdtqvlt nllrlmydwi dssreflfik daeelglykn imreacvkvs rslsrdmpph
     1321 klvimclrfa cawclkifqg gsdgeviske crrryrlghl alitlnrlsm sgnlaylcra
     1381 pegcpsteli riyinredev lfeilrryed ivteimtdws gvediqcdlk idrmddwflq
     1441 lmvtgsrwal erlkeivvyp sfrevlllaf erkknaiagt le
//
LOCUS       XP_077502509            1482 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113346 [Amblyomma americanum].
ACCESSION   XP_077502509
VERSION     XP_077502509.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646383.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1482
                     /product="uncharacterized protein LOC144113346"
                     /calculated_mol_wt=167526
     Region          518..1078
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1482
                     /gene="LOC144113346"
                     /coded_by="XM_077646383.1:2796..7244"
                     /db_xref="GeneID:144113346"
ORIGIN      
        1 meppakpllp gpmpltsafc ardrqdncre malrihclds gwgtptasql rrllglqcav
       61 dqvvagdcge vevyespnwt anvtlklers pkdsfmhivl kwcdqyqngs nngaaagksg
      121 ppewwlasee leefcdpyst qiatehkdvp vnafalgcfl gyntfvahfd stytmpppps
      181 plssakssss hsgksmsvkr gkkgrgakss cpsdgtsssa ktacpnselt spegfkvtqc
      241 nahfehcsng lylyfvdvrk rsstrfrllv rysticriil rktsptctiv ylqllhppll
      301 ykvcagnsat sqcnregdyl pqfdlsdttp wdravefgeg pfkcgadvlg rsrvmrltls
      361 gprhwaplcn lilrtrknaq vfyaplrelq arelkavarp svqdfgvmya llsvwsesfq
      421 vtdelamdaq scretllela ahaeecpral eealfeiyfs lsqgkvfwfr salehlyfry
      481 rahrqridvf tgsviaqelp shmvkvrkvi ltptrrvymp pqlvcksrll qhcdpdyalr
      541 ivvrdddckl isfslgackd dflsavikpp lssglkiggr rfmflgcsts qlrnhgiwfy
      601 ahdnqgrtae qirdgigdls tipsvpkfma rlgqafsqsl glltvprqnt sveqdirhaa
      661 pgrssdgshr eyifsdgigr ishsllrkvh haleleegee pcaiqirygg ckgmllldpt
      721 lsgcrivfre smrkfhsdhs dlyvlktskp rvlylnrpmi tileqsgika evflmlqnki
      781 ldsfidsmmd pheaahvlra ycalrlpyke lagvgidltv epffralvra vnkkvlkelr
      841 tkarilvppn ygrtmfgvld etgtleygqv fvqysndmlr ykvndpatil egdvivtknp
      901 cmhpgdirkl eavnvpqlhh vrdcivfpqk gerphpdema gsdldgdeys vlwyedlifn
      961 nncnpmhyys dppkerkasi gvqdmvdffc qyikgdnigl ianahlvwad ildgginshr
     1021 crelarkcav nldfakcgdl kglqnsekpp mypdfmekld tkntycsrkv lgqlyrnckk
     1081 velsteclev veeslpdprl llegreqfmk eatsaykrya kkirallksy rieseseals
     1141 gavsklskym kendstdmam vlesqvehvv rqtreeffse qldeahenlk asawyqvtye
     1201 lqsseggiqs fpwvvsdvlm rivvntsscl pvpasrnsfs qrlgalllrv phpgggdqdd
     1261 aqhgdtqvlt nllrlmydwi dssreflfik daeelglykn imreacvkvs rslsrdmpph
     1321 klvimclrfa cawclkifqg gsdgeviske crrryrlghl alitlnrlsm sgnlaylcra
     1381 pegcpsteli riyinredev lfeilrryed ivteimtdws gvediqcdlk idrmddwflq
     1441 lmvtgsrwal erlkeivvyp sfrevlllaf erkknaiagt le
//
LOCUS       XP_077502510             203 aa            linear   INV 25-NOV-2025
DEFINITION  protein big brother-like [Amblyomma americanum].
ACCESSION   XP_077502510
VERSION     XP_077502510.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646384.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (GKSP01015237.1) annotated
            using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 96 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..203
                     /product="protein big brother-like"
                     /calculated_mol_wt=23871
     Region          20..179
                     /region_name="CBF_beta"
                     /note="Core binding factor beta subunit; pfam02312"
                     /db_xref="CDD:396750"
     CDS             1..203
                     /gene="LOC144113350"
                     /coded_by="XM_077646384.1:154..765"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 96 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144113350"
ORIGIN      
        1 mlpfetpsif dqlprfvfkm prvvpdqkak fesdelfrrl sreceirytg yrdrpleerq
       61 vrfqnacreg htevafvatg tnlqlvfspc hngyvdccdf dkergkvhik ssfimngvcv
      121 ryrgwidldr ldgvgcleyd eergrledav lrdqiecynq rlrdfeekqr afrarherqa
      181 eaelevrrkh qelspdrggs asn
//
LOCUS       XP_077502511             828 aa            linear   INV 25-NOV-2025
DEFINITION  katanin p80 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502511
VERSION     XP_077502511.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646385.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..828
                     /product="katanin p80 isoform X1"
                     /calculated_mol_wt=89883
     Region          <40..279
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          52..88
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          94..130
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          135..171
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          178..213
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          219..255
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          261..296
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <387..>629
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          666..824
                     /region_name="Katanin_con80"
                     /note="con80 domain of Katanin; pfam13925"
                     /db_xref="CDD:464044"
     CDS             1..828
                     /gene="kat80"
                     /coded_by="XM_077646385.1:55..2541"
                     /db_xref="GeneID:144113351"
ORIGIN      
        1 mttglvaafp pvpmaplgsl aggrplpwlq letasycvgv eefvahgstv kclaighksg
       61 rvmvtggedn kvnlwaigkt ncimsltght tavecvkfcp aeemvcagst sgtakiwnle
      121 aakmvrtltg hkgnvrcmdf hphaefvasg smdttiklwd trkkecifty kghnkcvnsl
      181 kfspdgrwia sgsedgsvkl wdlpagkmls efrdhcgpvn dvdfhpnefl lasgssdstv
      241 kfwdlenfnl vsstegdsgi vrcvffspdg aclfsgaedf lkvygwepvk tcdtlvmgwn
      301 rvadisvaqn qliagafslt nvavyvidln rvqpfggmpn nssvpqyitt spfkvgshtr
      361 rsfskdkkdt adfqvmrmkp ledgdafple aeqgeanikd yrdynnifhp sqessldlgv
      421 qnkmsrtppk dnpfpapped pfkptlepqs skvvrlsgas npcvlterpg atvketrqls
      481 vttsysamgt tftirprgsl vgsgallppe ppapsimaai pgyssvgvvp nnipvptsvl
      541 pnkvgsramp tgpisavaas lgkpiekata mvrtsssqvv esstvsivrp vmsvlpdske
      601 altspqkavv hpeqkqsvdy vpsqrdtpag ldldeflprh lqaslrlgsa gipemseaea
      661 latiaeqhkt llavlhhrlk nikivlaqws tkdpkmaiet altmndqstl vdilniivlr
      721 pqiwtldmcq tilpaifgll qskyesymst gcaclrillk nfasviktni tappgvgvdi
      781 sreerynkcm scynqllsir sfllkrqtmq gklghlfrem hilmqgle
//
LOCUS       XP_077502512             818 aa            linear   INV 25-NOV-2025
DEFINITION  katanin p80 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502512
VERSION     XP_077502512.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646386.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..818
                     /product="katanin p80 isoform X2"
                     /calculated_mol_wt=88823
     Region          <40..279
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          52..88
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          94..130
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          135..171
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          178..213
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          219..255
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          261..296
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <411..>619
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          656..814
                     /region_name="Katanin_con80"
                     /note="con80 domain of Katanin; pfam13925"
                     /db_xref="CDD:464044"
     CDS             1..818
                     /gene="kat80"
                     /coded_by="XM_077646386.1:54..2510"
                     /db_xref="GeneID:144113351"
ORIGIN      
        1 mttglvaafp pvpmaplgsl aggrplpwlq letasycvgv eefvahgstv kclaighksg
       61 rvmvtggedn kvnlwaigkt ncimsltght tavecvkfcp aeemvcagst sgtakiwnle
      121 aakmvrtltg hkgnvrcmdf hphaefvasg smdttiklwd trkkecifty kghnkcvnsl
      181 kfspdgrwia sgsedgsvkl wdlpagkmls efrdhcgpvn dvdfhpnefl lasgssdstv
      241 kfwdlenfnl vsstegdsgi vrcvffspdg aclfsgaedf lkvygwepvk tcdtlvmgwn
      301 rvadisvaqn qliagafslt nvavyvidln rvqpfggmpn nssvpqyitt spfkvgshtr
      361 rsfskdkkdt adfqvmrmkp ledgdafple aeqgeanikd yrdynnifhp sqelsrtppk
      421 dnpfpapped pfkptlepqs skvvrlsgas npcvlterpg atvketrqls vttsysamgt
      481 tftirprgsl vgsgallppe ppapsimaai pgyssvgvvp nnipvptsvl pnkvgsramp
      541 tgpisavaas lgkpiekata mvrtsssqvv esstvsivrp vmsvlpdske altspqkavv
      601 hpeqkqsvdy vpsqrdtpag ldldeflprh lqaslrlgsa gipemseaea latiaeqhkt
      661 llavlhhrlk nikivlaqws tkdpkmaiet altmndqstl vdilniivlr pqiwtldmcq
      721 tilpaifgll qskyesymst gcaclrillk nfasviktni tappgvgvdi sreerynkcm
      781 scynqllsir sfllkrqtmq gklghlfrem hilmqgle
//
LOCUS       XP_077502513             810 aa            linear   INV 25-NOV-2025
DEFINITION  katanin p80 isoform X3 [Amblyomma americanum].
ACCESSION   XP_077502513
VERSION     XP_077502513.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646387.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..810
                     /product="katanin p80 isoform X3"
                     /calculated_mol_wt=87746
     Region          <40..279
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          52..88
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          94..130
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          135..171
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          178..213
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          219..255
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          261..296
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <332..>611
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          648..806
                     /region_name="Katanin_con80"
                     /note="con80 domain of Katanin; pfam13925"
                     /db_xref="CDD:464044"
     CDS             1..810
                     /gene="kat80"
                     /coded_by="XM_077646387.1:57..2489"
                     /db_xref="GeneID:144113351"
ORIGIN      
        1 mttglvaafp pvpmaplgsl aggrplpwlq letasycvgv eefvahgstv kclaighksg
       61 rvmvtggedn kvnlwaigkt ncimsltght tavecvkfcp aeemvcagst sgtakiwnle
      121 aakmvrtltg hkgnvrcmdf hphaefvasg smdttiklwd trkkecifty kghnkcvnsl
      181 kfspdgrwia sgsedgsvkl wdlpagkmls efrdhcgpvn dvdfhpnefl lasgssdstv
      241 kfwdlenfnl vsstegdsgi vrcvffspdg aclfsgaedf lkvygwepvk tcdtlvmgwn
      301 rvadisvaqn qliagafslt nvavyvidln rvqpfggmpn nssvpqyitt spfkvgvmrm
      361 kpledgdafp leaeqgeani kdyrdynnif hpsqessldl gvqnkmsrtp pkdnpfpapp
      421 edpfkptlep qsskvvrlsg asnpcvlter pgatvketrq lsvttsysam gttftirprg
      481 slvgsgallp peppapsima aipgyssvgv vpnnipvpts vlpnkvgsra mptgpisava
      541 aslgkpieka tamvrtsssq vvesstvsiv rpvmsvlpds kealtspqka vvhpeqkqsv
      601 dyvpsqrdtp agldldeflp rhlqaslrlg sagipemsea ealatiaeqh ktllavlhhr
      661 lknikivlaq wstkdpkmai etaltmndqs tlvdilniiv lrpqiwtldm cqtilpaifg
      721 llqskyesym stgcaclril lknfasvikt nitappgvgv disreerynk cmscynqlls
      781 irsfllkrqt mqgklghlfr emhilmqgle
//
LOCUS       XP_077502515             802 aa            linear   INV 25-NOV-2025
DEFINITION  katanin p80 isoform X4 [Amblyomma americanum].
ACCESSION   XP_077502515
VERSION     XP_077502515.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646389.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..802
                     /product="katanin p80 isoform X4"
                     /calculated_mol_wt=87126
     Region          <40..279
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          52..88
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          94..130
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          135..171
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          178..213
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          219..255
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          261..296
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          640..798
                     /region_name="Katanin_con80"
                     /note="con80 domain of Katanin; pfam13925"
                     /db_xref="CDD:464044"
     CDS             1..802
                     /gene="kat80"
                     /coded_by="XM_077646389.1:55..2463"
                     /db_xref="GeneID:144113351"
ORIGIN      
        1 mttglvaafp pvpmaplgsl aggrplpwlq letasycvgv eefvahgstv kclaighksg
       61 rvmvtggedn kvnlwaigkt ncimsltght tavecvkfcp aeemvcagst sgtakiwnle
      121 aakmvrtltg hkgnvrcmdf hphaefvasg smdttiklwd trkkecifty kghnkcvnsl
      181 kfspdgrwia sgsedgsvkl wdlpagkmls efrdhcgpvn dvdfhpnefl lasgssdstv
      241 kfwdlenfnl vsstegdsgi vrcvffspdg aclfsgaedf lkvygwepvk tcdtlvmgwn
      301 rvadisvaqn qliagafslt nvavyvidln rvqpfggmpn nssvpqyitt spfkvgshtr
      361 rsfskdkkdt adfqvmrmkp ledgdafple aeqgeanikd yrdynnifhp sqeypfkptl
      421 epqsskvvrl sgasnpcvlt erpgatvket rqlsvttsys amgttftirp rgslvgsgal
      481 lppeppapsi maaipgyssv gvvpnnipvp tsvlpnkvgs ramptgpisa vaaslgkpie
      541 katamvrtss sqvvesstvs ivrpvmsvlp dskealtspq kavvhpeqkq svdyvpsqrd
      601 tpagldldef lprhlqaslr lgsagipems eaealatiae qhktllavlh hrlknikivl
      661 aqwstkdpkm aietaltmnd qstlvdilni ivlrpqiwtl dmcqtilpai fgllqskyes
      721 ymstgcaclr illknfasvi ktnitappgv gvdisreery nkcmscynql lsirsfllkr
      781 qtmqgklghl fremhilmqg le
//
LOCUS       XP_077502516             789 aa            linear   INV 25-NOV-2025
DEFINITION  katanin p80 isoform X5 [Amblyomma americanum].
ACCESSION   XP_077502516
VERSION     XP_077502516.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646390.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..789
                     /product="katanin p80 isoform X5"
                     /calculated_mol_wt=86055
     Region          <11..250
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          23..59
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          65..101
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          106..142
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          149..184
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          190..226
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          232..267
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <382..>590
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          627..785
                     /region_name="Katanin_con80"
                     /note="con80 domain of Katanin; pfam13925"
                     /db_xref="CDD:464044"
     CDS             1..789
                     /gene="kat80"
                     /coded_by="XM_077646390.1:267..2636"
                     /db_xref="GeneID:144113351"
ORIGIN      
        1 makatkrawk lqefvahgst vkclaighks grvmvtgged nkvnlwaigk tncimsltgh
       61 ttavecvkfc paeemvcags tsgtakiwnl eaakmvrtlt ghkgnvrcmd fhphaefvas
      121 gsmdttiklw dtrkkecift ykghnkcvns lkfspdgrwi asgsedgsvk lwdlpagkml
      181 sefrdhcgpv ndvdfhpnef llasgssdst vkfwdlenfn lvsstegdsg ivrcvffspd
      241 gaclfsgaed flkvygwepv ktcdtlvmgw nrvadisvaq nqliagafsl tnvavyvidl
      301 nrvqpfggmp nnssvpqyit tspfkvgsht rrsfskdkkd tadfqvmrmk pledgdafpl
      361 eaeqgeanik dyrdynnifh psqelsrtpp kdnpfpappe dpfkptlepq sskvvrlsga
      421 snpcvlterp gatvketrql svttsysamg ttftirprgs lvgsgallpp eppapsimaa
      481 ipgyssvgvv pnnipvptsv lpnkvgsram ptgpisavaa slgkpiekat amvrtsssqv
      541 vesstvsivr pvmsvlpdsk ealtspqkav vhpeqkqsvd yvpsqrdtpa gldldeflpr
      601 hlqaslrlgs agipemseae alatiaeqhk tllavlhhrl knikivlaqw stkdpkmaie
      661 taltmndqst lvdilniivl rpqiwtldmc qtilpaifgl lqskyesyms tgcaclrill
      721 knfasviktn itappgvgvd isreerynkc mscynqllsi rsfllkrqtm qgklghlfre
      781 mhilmqgle
//
LOCUS       XP_077502517             773 aa            linear   INV 25-NOV-2025
DEFINITION  katanin p80 isoform X6 [Amblyomma americanum].
ACCESSION   XP_077502517
VERSION     XP_077502517.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646391.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..773
                     /product="katanin p80 isoform X6"
                     /calculated_mol_wt=84358
     Region          <11..250
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          23..59
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          65..101
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          106..142
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          149..184
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          190..226
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          232..267
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          611..769
                     /region_name="Katanin_con80"
                     /note="con80 domain of Katanin; pfam13925"
                     /db_xref="CDD:464044"
     CDS             1..773
                     /gene="kat80"
                     /coded_by="XM_077646391.1:264..2585"
                     /db_xref="GeneID:144113351"
ORIGIN      
        1 makatkrawk lqefvahgst vkclaighks grvmvtgged nkvnlwaigk tncimsltgh
       61 ttavecvkfc paeemvcags tsgtakiwnl eaakmvrtlt ghkgnvrcmd fhphaefvas
      121 gsmdttiklw dtrkkecift ykghnkcvns lkfspdgrwi asgsedgsvk lwdlpagkml
      181 sefrdhcgpv ndvdfhpnef llasgssdst vkfwdlenfn lvsstegdsg ivrcvffspd
      241 gaclfsgaed flkvygwepv ktcdtlvmgw nrvadisvaq nqliagafsl tnvavyvidl
      301 nrvqpfggmp nnssvpqyit tspfkvgsht rrsfskdkkd tadfqvmrmk pledgdafpl
      361 eaeqgeanik dyrdynnifh psqeypfkpt lepqsskvvr lsgasnpcvl terpgatvke
      421 trqlsvttsy samgttftir prgslvgsga llppeppaps imaaipgyss vgvvpnnipv
      481 ptsvlpnkvg sramptgpis avaaslgkpi ekatamvrts ssqvvesstv sivrpvmsvl
      541 pdskealtsp qkavvhpeqk qsvdyvpsqr dtpagldlde flprhlqasl rlgsagipem
      601 seaealatia eqhktllavl hhrlknikiv laqwstkdpk maietaltmn dqstlvdiln
      661 iivlrpqiwt ldmcqtilpa ifgllqskye symstgcacl rillknfasv iktnitappg
      721 vgvdisreer ynkcmscynq llsirsfllk rqtmqgklgh lfremhilmq gle
//
LOCUS       XP_077502518             256 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin E2 variant domain-containing protein pendolino isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077502518
VERSION     XP_077502518.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646392.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..256
                     /product="ubiquitin E2 variant domain-containing protein
                     pendolino isoform X1"
                     /calculated_mol_wt=29283
     Region          43..155
                     /region_name="UEV_AKTIP"
                     /note="ubiquitin E2 variant (UEV) domain of
                     AKT-interacting protein and related proteins; cd23814"
                     /db_xref="CDD:467434"
     CDS             1..256
                     /gene="peo"
                     /coded_by="XM_077646392.1:732..1502"
                     /db_xref="GeneID:144113352"
ORIGIN      
        1 msvrtsrkml patpeqrvgg rclqplehds qcshaygrvf leyslmseyl mlmrqqlsgv
       61 yvmpsassaf twfgvlfirq glyqggvfrf tlripdnypd gdcpdltfes pifhplidpi
      121 sgdmdikrsf pkwrknvnyl wqvllcarra fykietqmpl npeaatlyeq nteqfklkva
      181 eslaeceqrl yespamddsh alhflpwdvt vqervaqrhi ldkveaadea leespsntgl
      241 swvkhgcmqv fsktas
//
LOCUS       XP_077502519             256 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin E2 variant domain-containing protein pendolino isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077502519
VERSION     XP_077502519.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646393.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..256
                     /product="ubiquitin E2 variant domain-containing protein
                     pendolino isoform X1"
                     /calculated_mol_wt=29283
     Region          43..155
                     /region_name="UEV_AKTIP"
                     /note="ubiquitin E2 variant (UEV) domain of
                     AKT-interacting protein and related proteins; cd23814"
                     /db_xref="CDD:467434"
     CDS             1..256
                     /gene="peo"
                     /coded_by="XM_077646393.1:286..1056"
                     /db_xref="GeneID:144113352"
ORIGIN      
        1 msvrtsrkml patpeqrvgg rclqplehds qcshaygrvf leyslmseyl mlmrqqlsgv
       61 yvmpsassaf twfgvlfirq glyqggvfrf tlripdnypd gdcpdltfes pifhplidpi
      121 sgdmdikrsf pkwrknvnyl wqvllcarra fykietqmpl npeaatlyeq nteqfklkva
      181 eslaeceqrl yespamddsh alhflpwdvt vqervaqrhi ldkveaadea leespsntgl
      241 swvkhgcmqv fsktas
//
LOCUS       XP_077502520             256 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin E2 variant domain-containing protein pendolino isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077502520
VERSION     XP_077502520.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646394.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..256
                     /product="ubiquitin E2 variant domain-containing protein
                     pendolino isoform X1"
                     /calculated_mol_wt=29283
     Region          43..155
                     /region_name="UEV_AKTIP"
                     /note="ubiquitin E2 variant (UEV) domain of
                     AKT-interacting protein and related proteins; cd23814"
                     /db_xref="CDD:467434"
     CDS             1..256
                     /gene="peo"
                     /coded_by="XM_077646394.1:264..1034"
                     /db_xref="GeneID:144113352"
ORIGIN      
        1 msvrtsrkml patpeqrvgg rclqplehds qcshaygrvf leyslmseyl mlmrqqlsgv
       61 yvmpsassaf twfgvlfirq glyqggvfrf tlripdnypd gdcpdltfes pifhplidpi
      121 sgdmdikrsf pkwrknvnyl wqvllcarra fykietqmpl npeaatlyeq nteqfklkva
      181 eslaeceqrl yespamddsh alhflpwdvt vqervaqrhi ldkveaadea leespsntgl
      241 swvkhgcmqv fsktas
//
LOCUS       XP_077502521             256 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin E2 variant domain-containing protein pendolino isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077502521
VERSION     XP_077502521.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646395.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..256
                     /product="ubiquitin E2 variant domain-containing protein
                     pendolino isoform X1"
                     /calculated_mol_wt=29283
     Region          43..155
                     /region_name="UEV_AKTIP"
                     /note="ubiquitin E2 variant (UEV) domain of
                     AKT-interacting protein and related proteins; cd23814"
                     /db_xref="CDD:467434"
     CDS             1..256
                     /gene="peo"
                     /coded_by="XM_077646395.1:97..867"
                     /db_xref="GeneID:144113352"
ORIGIN      
        1 msvrtsrkml patpeqrvgg rclqplehds qcshaygrvf leyslmseyl mlmrqqlsgv
       61 yvmpsassaf twfgvlfirq glyqggvfrf tlripdnypd gdcpdltfes pifhplidpi
      121 sgdmdikrsf pkwrknvnyl wqvllcarra fykietqmpl npeaatlyeq nteqfklkva
      181 eslaeceqrl yespamddsh alhflpwdvt vqervaqrhi ldkveaadea leespsntgl
      241 swvkhgcmqv fsktas
//
LOCUS       XP_077502522             256 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin E2 variant domain-containing protein pendolino isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077502522
VERSION     XP_077502522.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646396.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..256
                     /product="ubiquitin E2 variant domain-containing protein
                     pendolino isoform X1"
                     /calculated_mol_wt=29283
     Region          43..155
                     /region_name="UEV_AKTIP"
                     /note="ubiquitin E2 variant (UEV) domain of
                     AKT-interacting protein and related proteins; cd23814"
                     /db_xref="CDD:467434"
     CDS             1..256
                     /gene="peo"
                     /coded_by="XM_077646396.1:520..1290"
                     /db_xref="GeneID:144113352"
ORIGIN      
        1 msvrtsrkml patpeqrvgg rclqplehds qcshaygrvf leyslmseyl mlmrqqlsgv
       61 yvmpsassaf twfgvlfirq glyqggvfrf tlripdnypd gdcpdltfes pifhplidpi
      121 sgdmdikrsf pkwrknvnyl wqvllcarra fykietqmpl npeaatlyeq nteqfklkva
      181 eslaeceqrl yespamddsh alhflpwdvt vqervaqrhi ldkveaadea leespsntgl
      241 swvkhgcmqv fsktas
//
LOCUS       XP_077502523             248 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin E2 variant domain-containing protein pendolino isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077502523
VERSION     XP_077502523.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646397.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..248
                     /product="ubiquitin E2 variant domain-containing protein
                     pendolino isoform X2"
                     /calculated_mol_wt=28336
     Region          35..147
                     /region_name="UEV_AKTIP"
                     /note="ubiquitin E2 variant (UEV) domain of
                     AKT-interacting protein and related proteins; cd23814"
                     /db_xref="CDD:467434"
     CDS             1..248
                     /gene="peo"
                     /coded_by="XM_077646397.1:527..1273"
                     /db_xref="GeneID:144113352"
ORIGIN      
        1 mlpatpeqrv ggrclqpleh dsqcshaygr vfleyslmse ylmlmrqqls gvyvmpsass
       61 aftwfgvlfi rqglyqggvf rftlripdny pdgdcpdltf espifhplid pisgdmdikr
      121 sfpkwrknvn ylwqvllcar rafykietqm plnpeaatly eqnteqfklk vaeslaeceq
      181 rlyespamdd shalhflpwd vtvqervaqr hildkveaad ealeespsnt glswvkhgcm
      241 qvfsktas
//
LOCUS       XP_077502524             226 aa            linear   INV 25-NOV-2025
DEFINITION  UPF0598 protein CG30010 [Amblyomma americanum].
ACCESSION   XP_077502524
VERSION     XP_077502524.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646398.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..226
                     /product="UPF0598 protein CG30010"
                     /calculated_mol_wt=26512
     Region          28..211
                     /region_name="DUF4505"
                     /note="Domain of unknown function (DUF4505); pfam14956"
                     /db_xref="CDD:464402"
     CDS             1..226
                     /gene="LOC144113354"
                     /coded_by="XM_077646398.1:163..843"
                     /db_xref="GeneID:144113354"
ORIGIN      
        1 mvkwrtlgwk lyhrlptrvc rrslhyeqgq ttkeytreyf yfidhqgmlf lddsrmknft
       61 scfkekkfle fffrrlrrne tgkyedefpf aspcgremnf vrcddlpfvf thivqlpeed
      121 vkqknskgfl lhnhagnllk veftpehlcm pagtgrvyhp apervggvgl vrsllaiels
      181 khftfpsdsd kcppthfdwe gtrykltnsl lpiisevscw sgtnel
//
LOCUS       XP_077502525             742 aa            linear   INV 25-NOV-2025
DEFINITION  cell adhesion molecule Dscam1-like [Amblyomma americanum].
ACCESSION   XP_077502525
VERSION     XP_077502525.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646399.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..742
                     /product="cell adhesion molecule Dscam1-like"
                     /calculated_mol_wt=80302
     Region          30..115
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          42..46
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          56..60
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          81..85
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          95..100
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          108..111
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          119..216
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          136..140
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          149..153
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          181..185
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          195..200
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          209..212
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          227..317
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          237..241
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          250..254
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          283..287
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          297..302
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          <320..>533
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Region          321..410
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(321,383,398)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(399..400,402..403)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          519..607
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(519,582,597)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(598..599,601..602)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     CDS             1..742
                     /gene="LOC144113355"
                     /coded_by="XM_077646399.1:295..2523"
                     /db_xref="GeneID:144113355"
ORIGIN      
        1 mvefktfvll cvcsrltftl aqkppklspl qlpkdlvagt dvrltcnvvv gdtpleilwy
       61 rnekrlissp fieeeagthl stlsfkplge dhggtykcma rnaagvdsas velvvnaapr
      121 wvvepsdvka aegsavtvhc nvkgtpvpqv awahdnggrv aqlrsmsgne dsdrhkllsn
      181 gtllirdvqk sdsgmytcsa dngigsalkk avavtvytva qaqvltqhls vqfgqtanlt
      241 ciatgdhpit vtwlkgeksv atnsqlydrv ivsndtqker lvsslilqqv tagdagrytc
      301 rvknayaedt kairlnvqqp psnptevevs dvwsrsarir wksptsstvl syqvrfwshr
      361 edemlnhtvr ggvttalvrd lhpateyrva ivavnsagas esspiirftt tqeepsaapv
      421 nvrieksgat fvlvtwsppp esdwngellg yyvgyssaae ssrkspfsyh tvgpdqgsft
      481 lrglakatsy vvvvkafnav gsgppspaip vttldgdfpp aptlsvsavd qtslqvtwmf
      541 nvpvphritg ytlhyrqpgd swkevfvasg kqssyllsgl epslpcqvyl iahsrqgnse
      601 psdvlnvplv vtsipqqpgs passdtelre vlyvvvpivt atalavvlvv svcfclyvrk
      661 trppppppay adfprdsdft favhgmpppg katpystmqr stdnegiyes ivdmrtlslr
      721 rkqaaqnslr kgsmdlvdvc vv
//
LOCUS       XP_077502526             865 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113356 [Amblyomma americanum].
ACCESSION   XP_077502526
VERSION     XP_077502526.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646400.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..865
                     /product="uncharacterized protein LOC144113356"
                     /calculated_mol_wt=95464
     CDS             1..865
                     /gene="LOC144113356"
                     /coded_by="XM_077646400.1:378..2975"
                     /db_xref="GeneID:144113356"
ORIGIN      
        1 mlemfycglc tysstskahl gvhayfahda kpvpwykkns daepvlpped fnnlvhrkry
       61 ictlttachf scltneifrg hleshedcst yrcrlcfgst dtcgllfhhy qtdhgyfavq
      121 clycdfgsnd dfivmhhvme qhskrpfril lrngdtsavs qelrllqeyl tedpvmpaqp
      181 caqlsvealt qenvtssnaa mpradpvklg hssleaklsl dhsckgvsnm dseveeelve
      241 nhaassavcv thqsagrasp lsteeslyss ssectqhlat qlwgtkslhp syhveqslql
      301 dpgkaqplhe tetssslnds whlycsaenc svefkdifdy lnhlaechsl qssfpcpkcd
      361 ltvsstdlpm hlvgkhsgfl fcpyqdcsfg scdqcgldth iiqahqiyre eknrngahva
      421 seqglcttsn sssknhtstr nvdftsemei pngesrndkl ccevslenak ddltnrlyic
      481 gscstnystl tcylhhiasa hlvpficghc fkpykksrcl lmhagclhlg qpfsvlclkd
      541 gkvtdiglnv aplwieeaqg ylrmvqpgkm rakgktmlkn lkravtyiss aclenvasqa
      601 ellqsnavph saslklvhga egtslqyert vdtvrsdttv lsrrsvpsql lydsapagvv
      661 sngssnssen qltvaphtsp phlqnassst sqftnarnvk wkilksvyid assvkkvngn
      721 ivvdsgvlrr lnsqlpvssp pvrkvsgslc crvneglhvc secfltfstf eelakhiklh
      781 sldiingfdl asgvlsigds sglegltfqh dikqsrdtaq lqlkpvqsev qkrvvdnrrl
      841 clstqqkceg aiqsvftcge fesyc
//
LOCUS       XP_077502528             865 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113356 [Amblyomma americanum].
ACCESSION   XP_077502528
VERSION     XP_077502528.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646402.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..865
                     /product="uncharacterized protein LOC144113356"
                     /calculated_mol_wt=95464
     CDS             1..865
                     /gene="LOC144113356"
                     /coded_by="XM_077646402.1:944..3541"
                     /db_xref="GeneID:144113356"
ORIGIN      
        1 mlemfycglc tysstskahl gvhayfahda kpvpwykkns daepvlpped fnnlvhrkry
       61 ictlttachf scltneifrg hleshedcst yrcrlcfgst dtcgllfhhy qtdhgyfavq
      121 clycdfgsnd dfivmhhvme qhskrpfril lrngdtsavs qelrllqeyl tedpvmpaqp
      181 caqlsvealt qenvtssnaa mpradpvklg hssleaklsl dhsckgvsnm dseveeelve
      241 nhaassavcv thqsagrasp lsteeslyss ssectqhlat qlwgtkslhp syhveqslql
      301 dpgkaqplhe tetssslnds whlycsaenc svefkdifdy lnhlaechsl qssfpcpkcd
      361 ltvsstdlpm hlvgkhsgfl fcpyqdcsfg scdqcgldth iiqahqiyre eknrngahva
      421 seqglcttsn sssknhtstr nvdftsemei pngesrndkl ccevslenak ddltnrlyic
      481 gscstnystl tcylhhiasa hlvpficghc fkpykksrcl lmhagclhlg qpfsvlclkd
      541 gkvtdiglnv aplwieeaqg ylrmvqpgkm rakgktmlkn lkravtyiss aclenvasqa
      601 ellqsnavph saslklvhga egtslqyert vdtvrsdttv lsrrsvpsql lydsapagvv
      661 sngssnssen qltvaphtsp phlqnassst sqftnarnvk wkilksvyid assvkkvngn
      721 ivvdsgvlrr lnsqlpvssp pvrkvsgslc crvneglhvc secfltfstf eelakhiklh
      781 sldiingfdl asgvlsigds sglegltfqh dikqsrdtaq lqlkpvqsev qkrvvdnrrl
      841 clstqqkceg aiqsvftcge fesyc
//
LOCUS       XP_077502529             151 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113357 [Amblyomma americanum].
ACCESSION   XP_077502529
VERSION     XP_077502529.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646403.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..151
                     /product="uncharacterized protein LOC144113357"
                     /calculated_mol_wt=16702
     Region          66..>121
                     /region_name="TIM"
                     /note="TIM-like beta/alpha barrel domains; cl21457"
                     /db_xref="CDD:473867"
     CDS             1..151
                     /gene="LOC144113357"
                     /coded_by="XM_077646403.1:60..515"
                     /db_xref="GeneID:144113357"
ORIGIN      
        1 mpgvlshtev rmgkytkmnf rvsrtvamll tegfeaspvn prsdnggspa ktaeehvltf
       61 lwyaanktcl rdvaerfslg ettafriier mieylrevpk tvilfpadle qlaknfeqvl
      121 vylvplaast apilafaarr trsgqhistg t
//
LOCUS       XP_077502530             145 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113358 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502530
VERSION     XP_077502530.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646404.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..145
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..145
                     /product="uncharacterized protein LOC144113358 isoform X1"
                     /calculated_mol_wt=16267
     CDS             1..145
                     /gene="LOC144113358"
                     /coded_by="XM_077646404.1:144..581"
                     /db_xref="GeneID:144113358"
ORIGIN      
        1 mepfhlhnty dfnhgsrcsf fltaaakqek kgitarrrvf vsiyrldccw yqdplqgrpl
       61 pricsgrlks gimtcvfiev qtatasttyp taphyqqrqh kvekavygtm kgipkdavfh
      121 kraattiwst eavaqcssss kfsge
//
LOCUS       XP_077502531             131 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113358 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502531
VERSION     XP_077502531.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646405.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..131
                     /product="uncharacterized protein LOC144113358 isoform X2"
                     /calculated_mol_wt=14728
     CDS             1..131
                     /gene="LOC144113358"
                     /coded_by="XM_077646405.1:887..1282"
                     /db_xref="GeneID:144113358"
ORIGIN      
        1 mkqvylsyya tpmlvgalcn impssvptal lhdlsrmlfs klmtihsdwm nllftsqpls
       61 tylqtihsqr mslqhyltaf kvirgcadke eeppsspghr lpigdkkssq ggdisavcdf
      121 ermnwqhink v
//
LOCUS       XP_077502532             131 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113358 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502532
VERSION     XP_077502532.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646406.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..131
                     /product="uncharacterized protein LOC144113358 isoform X2"
                     /calculated_mol_wt=14728
     CDS             1..131
                     /gene="LOC144113358"
                     /coded_by="XM_077646406.1:887..1282"
                     /db_xref="GeneID:144113358"
ORIGIN      
        1 mkqvylsyya tpmlvgalcn impssvptal lhdlsrmlfs klmtihsdwm nllftsqpls
       61 tylqtihsqr mslqhyltaf kvirgcadke eeppsspghr lpigdkkssq ggdisavcdf
      121 ermnwqhink v
//
LOCUS       XP_077502533             128 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113358 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502533
VERSION     XP_077502533.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646407.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..128
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..128
                     /product="uncharacterized protein LOC144113358 isoform X3"
                     /calculated_mol_wt=14215
     CDS             1..128
                     /gene="LOC144113358"
                     /coded_by="XM_077646407.1:887..1273"
                     /db_xref="GeneID:144113358"
ORIGIN      
        1 mkqvylsyya tpmlvgalcn impssvptal lhdlsrmlfs klmtihsdwm nllftsqpls
       61 tylqtihsqr mslqhyltaf kvirgcadke eeppsspghr lpigdkkssq eclnitalpg
      121 ppsgtcfw
//
LOCUS       XP_077502534             111 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113358 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077502534
VERSION     XP_077502534.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646408.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..111
                     /product="uncharacterized protein LOC144113358 isoform X4"
                     /calculated_mol_wt=12432
     CDS             1..111
                     /gene="LOC144113358"
                     /coded_by="XM_077646408.1:887..1222"
                     /db_xref="GeneID:144113358"
ORIGIN      
        1 mkqvylsyya tpmlvgalcn impssvptal lhdlsrmlfs klilkagrim rkrlslstll
       61 crgyktfgiw rdkevsglps lrvicedrkr lsggvltkrr nhhlaqaiac q
//
LOCUS       XP_077502535             108 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113358 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077502535
VERSION     XP_077502535.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646409.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..108
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..108
                     /product="uncharacterized protein LOC144113358 isoform X5"
                     /calculated_mol_wt=11979
     CDS             1..108
                     /gene="LOC144113358"
                     /coded_by="XM_077646409.1:887..1213"
                     /db_xref="GeneID:144113358"
ORIGIN      
        1 mkqvylsyya tpmlvgalcn impssvptal lhdlsrmlfs klmtihsdwm nllftsqpvi
       61 rgcadkeeep psspghrlpi gdkkssqggd isavcdferm nwqhinkv
//
LOCUS       XP_077502537             147 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113360 [Amblyomma americanum].
ACCESSION   XP_077502537
VERSION     XP_077502537.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646411.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..147
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..147
                     /product="uncharacterized protein LOC144113360"
                     /calculated_mol_wt=15981
     CDS             1..147
                     /gene="LOC144113360"
                     /coded_by="XM_077646411.1:1599..2042"
                     /db_xref="GeneID:144113360"
ORIGIN      
        1 mrttgypgyp ahsasvattp calqalcgas twcaqtsvys lrgrqrplka qhksagdlrq
       61 hqgprpaaat rrgpcgscge gvvpcwfita dakvsrrwln rrhqllnrkl tatvkrmpsv
      121 filnpdvstd mlvlsytfgv nrtqkgk
//
LOCUS       XP_077502538             620 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113361 [Amblyomma americanum].
ACCESSION   XP_077502538
VERSION     XP_077502538.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646412.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..620
                     /product="uncharacterized protein LOC144113361"
                     /calculated_mol_wt=69906
     CDS             1..620
                     /gene="LOC144113361"
                     /coded_by="XM_077646412.1:76..1938"
                     /db_xref="GeneID:144113361"
ORIGIN      
        1 mpkrkckfte qlgkeypfik ktkseadvrc gacgvdfsva hggrsdivkh issvkhkqsv
       61 saaasssavt rffrqerage kelqlaaseg ifafhtiahn hsfrsmdcts klvqklfsek
      121 fscartkaea ialnvlapsa eeelktdlqe akfvsvfcda snhkdlkvlp imvryftaaa
      181 gvqtkllein tlpgetsamv sqyimdalea nqvtsklvaf sadnancnfg gaarngknnv
      241 fsilqdsvdh kilgigcaah ivhntvqtaa dclpldieav ivkiysyfyi ytvrvesfke
      301 fcdfasveyq rllgysktrw lalmpaverv lklypalkay flsidkcpsl iksffedpcg
      361 elwlyflhsq aamfestvlq iegdtktave vysalsqlqt kvqqryeqsy lpssvrsrat
      421 eleeqgqvrr eqvvntvvkf yatcasyldr wcqqfdevrs llwvslvssp cwddvqktve
      481 litsefnhvt vdenamfdei nnvshnvtae kikewktqki svgerwveil ghfekndvpc
      541 enlktvleyc mclpgtnapv ervfslmnki wtaektqlkv atlkamlvlk vnvrmtceqy
      601 fahlckkehl lkkihssqky
//
LOCUS       XP_077502539             917 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113362 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502539
VERSION     XP_077502539.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646413.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..917
                     /product="uncharacterized protein LOC144113362 isoform X1"
                     /calculated_mol_wt=101200
     CDS             1..917
                     /gene="LOC144113362"
                     /coded_by="XM_077646413.1:469..3222"
                     /db_xref="GeneID:144113362"
ORIGIN      
        1 mfycglctys stskallgvh ayfthdakpv pwyknnsdae halpldefkn lvhrkryfct
       61 ltttchfscl tndifrqhlv shkdfstykc hlcigstytc gllfhhyqtd hgffavqcly
      121 cdfgnnddwi vmrhvmeqhs rrpfrillrn gdvpavsqel rlliqeylte dpvmpsqpct
      181 qlsvealtqe nvtssnepms gvgpslpcaq lslealtqvn vtssnaampr adlvnlghss
      241 leaklsldds ckgvsnmdse veeklvenha atfavcvthq sagrtsplst geglhssssd
      301 ftqrlstelq gtkslhpsch veqnlelepg kaqplqetet ssslndswhl acsaencsve
      361 fgdifdylnh laechplqsf pcpkcdltvs stdlsmhlve khggflfcpy qdcsfgspdq
      421 cgldthiiqa hqiyreenrn gahvaneqgl cttsnsssrn htstgsvdft semeisnrei
      481 rddklccevn ledakddltn rlyicglcsa nystltsylh htasahsvpf icghcfkpyk
      541 rsrcllmhag clhlgqpfsv lclkdgketd iglnvaplwi keaqeylrmv qpgklqakgk
      601 tmlknlkrav tyisstflen vssqaellqs navshsaslk lvhgaertsl qyersvdtvc
      661 sdatvssgrs vpsqllydpa pvgvvcngss nssenqltav phlfknldis vdtspphlqn
      721 nassstsqft iarsvkckil qsvyidassi kkvngnivvd sgvlrrlnsq lpvssppvrk
      781 vsgslccrvn eglhvcsecf ltfstfeela khivklhsld iingfdlasa vlsigdssdl
      841 qglriqlgik qsrgtaqlqf krvqsevqkr evdnnglfvc lhnrsvkvpy sqfsravnln
      901 pivklvrlpi ikkepke
//
LOCUS       XP_077502540             917 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113362 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502540
VERSION     XP_077502540.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646414.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..917
                     /product="uncharacterized protein LOC144113362 isoform X1"
                     /calculated_mol_wt=101200
     CDS             1..917
                     /gene="LOC144113362"
                     /coded_by="XM_077646414.1:517..3270"
                     /db_xref="GeneID:144113362"
ORIGIN      
        1 mfycglctys stskallgvh ayfthdakpv pwyknnsdae halpldefkn lvhrkryfct
       61 ltttchfscl tndifrqhlv shkdfstykc hlcigstytc gllfhhyqtd hgffavqcly
      121 cdfgnnddwi vmrhvmeqhs rrpfrillrn gdvpavsqel rlliqeylte dpvmpsqpct
      181 qlsvealtqe nvtssnepms gvgpslpcaq lslealtqvn vtssnaampr adlvnlghss
      241 leaklsldds ckgvsnmdse veeklvenha atfavcvthq sagrtsplst geglhssssd
      301 ftqrlstelq gtkslhpsch veqnlelepg kaqplqetet ssslndswhl acsaencsve
      361 fgdifdylnh laechplqsf pcpkcdltvs stdlsmhlve khggflfcpy qdcsfgspdq
      421 cgldthiiqa hqiyreenrn gahvaneqgl cttsnsssrn htstgsvdft semeisnrei
      481 rddklccevn ledakddltn rlyicglcsa nystltsylh htasahsvpf icghcfkpyk
      541 rsrcllmhag clhlgqpfsv lclkdgketd iglnvaplwi keaqeylrmv qpgklqakgk
      601 tmlknlkrav tyisstflen vssqaellqs navshsaslk lvhgaertsl qyersvdtvc
      661 sdatvssgrs vpsqllydpa pvgvvcngss nssenqltav phlfknldis vdtspphlqn
      721 nassstsqft iarsvkckil qsvyidassi kkvngnivvd sgvlrrlnsq lpvssppvrk
      781 vsgslccrvn eglhvcsecf ltfstfeela khivklhsld iingfdlasa vlsigdssdl
      841 qglriqlgik qsrgtaqlqf krvqsevqkr evdnnglfvc lhnrsvkvpy sqfsravnln
      901 pivklvrlpi ikkepke
//
LOCUS       XP_077502541             917 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113362 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502541
VERSION     XP_077502541.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646415.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..917
                     /product="uncharacterized protein LOC144113362 isoform X1"
                     /calculated_mol_wt=101200
     CDS             1..917
                     /gene="LOC144113362"
                     /coded_by="XM_077646415.1:441..3194"
                     /db_xref="GeneID:144113362"
ORIGIN      
        1 mfycglctys stskallgvh ayfthdakpv pwyknnsdae halpldefkn lvhrkryfct
       61 ltttchfscl tndifrqhlv shkdfstykc hlcigstytc gllfhhyqtd hgffavqcly
      121 cdfgnnddwi vmrhvmeqhs rrpfrillrn gdvpavsqel rlliqeylte dpvmpsqpct
      181 qlsvealtqe nvtssnepms gvgpslpcaq lslealtqvn vtssnaampr adlvnlghss
      241 leaklsldds ckgvsnmdse veeklvenha atfavcvthq sagrtsplst geglhssssd
      301 ftqrlstelq gtkslhpsch veqnlelepg kaqplqetet ssslndswhl acsaencsve
      361 fgdifdylnh laechplqsf pcpkcdltvs stdlsmhlve khggflfcpy qdcsfgspdq
      421 cgldthiiqa hqiyreenrn gahvaneqgl cttsnsssrn htstgsvdft semeisnrei
      481 rddklccevn ledakddltn rlyicglcsa nystltsylh htasahsvpf icghcfkpyk
      541 rsrcllmhag clhlgqpfsv lclkdgketd iglnvaplwi keaqeylrmv qpgklqakgk
      601 tmlknlkrav tyisstflen vssqaellqs navshsaslk lvhgaertsl qyersvdtvc
      661 sdatvssgrs vpsqllydpa pvgvvcngss nssenqltav phlfknldis vdtspphlqn
      721 nassstsqft iarsvkckil qsvyidassi kkvngnivvd sgvlrrlnsq lpvssppvrk
      781 vsgslccrvn eglhvcsecf ltfstfeela khivklhsld iingfdlasa vlsigdssdl
      841 qglriqlgik qsrgtaqlqf krvqsevqkr evdnnglfvc lhnrsvkvpy sqfsravnln
      901 pivklvrlpi ikkepke
//
LOCUS       XP_077502542             917 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113362 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502542
VERSION     XP_077502542.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646416.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..917
                     /product="uncharacterized protein LOC144113362 isoform X1"
                     /calculated_mol_wt=101200
     CDS             1..917
                     /gene="LOC144113362"
                     /coded_by="XM_077646416.1:393..3146"
                     /db_xref="GeneID:144113362"
ORIGIN      
        1 mfycglctys stskallgvh ayfthdakpv pwyknnsdae halpldefkn lvhrkryfct
       61 ltttchfscl tndifrqhlv shkdfstykc hlcigstytc gllfhhyqtd hgffavqcly
      121 cdfgnnddwi vmrhvmeqhs rrpfrillrn gdvpavsqel rlliqeylte dpvmpsqpct
      181 qlsvealtqe nvtssnepms gvgpslpcaq lslealtqvn vtssnaampr adlvnlghss
      241 leaklsldds ckgvsnmdse veeklvenha atfavcvthq sagrtsplst geglhssssd
      301 ftqrlstelq gtkslhpsch veqnlelepg kaqplqetet ssslndswhl acsaencsve
      361 fgdifdylnh laechplqsf pcpkcdltvs stdlsmhlve khggflfcpy qdcsfgspdq
      421 cgldthiiqa hqiyreenrn gahvaneqgl cttsnsssrn htstgsvdft semeisnrei
      481 rddklccevn ledakddltn rlyicglcsa nystltsylh htasahsvpf icghcfkpyk
      541 rsrcllmhag clhlgqpfsv lclkdgketd iglnvaplwi keaqeylrmv qpgklqakgk
      601 tmlknlkrav tyisstflen vssqaellqs navshsaslk lvhgaertsl qyersvdtvc
      661 sdatvssgrs vpsqllydpa pvgvvcngss nssenqltav phlfknldis vdtspphlqn
      721 nassstsqft iarsvkckil qsvyidassi kkvngnivvd sgvlrrlnsq lpvssppvrk
      781 vsgslccrvn eglhvcsecf ltfstfeela khivklhsld iingfdlasa vlsigdssdl
      841 qglriqlgik qsrgtaqlqf krvqsevqkr evdnnglfvc lhnrsvkvpy sqfsravnln
      901 pivklvrlpi ikkepke
//
LOCUS       XP_077502543             111 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113362 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502543
VERSION     XP_077502543.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646417.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..111
                     /product="uncharacterized protein LOC144113362 isoform X2"
                     /calculated_mol_wt=12327
     CDS             1..111
                     /gene="LOC144113362"
                     /coded_by="XM_077646417.1:73..408"
                     /db_xref="GeneID:144113362"
ORIGIN      
        1 mlllcfrqsr lcdskiaais nrlvlrdgvt rspvvallly rlpvfyrtav htgngryrdg
       61 shlkplarlt yccivlydke ekgvvtalvt ivaayillyy kcsvasetve a
//
LOCUS       XP_077502544              95 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113362 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502544
VERSION     XP_077502544.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646418.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..95
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..95
                     /product="uncharacterized protein LOC144113362 isoform X3"
                     /calculated_mol_wt=10555
     CDS             1..95
                     /gene="LOC144113362"
                     /coded_by="XM_077646418.1:73..360"
                     /db_xref="GeneID:144113362"
ORIGIN      
        1 mlllcfrqsr lcdskiaais nrlvlrdgvt rspvvallly rlpvfyrtav htgngryrvl
       61 ydkeekgvvt alvtivaayi llyykcsvas etvea
//
LOCUS       XP_077502545             371 aa            linear   INV 25-NOV-2025
DEFINITION  acid phosphatase 1 [Amblyomma americanum].
ACCESSION   XP_077502545
VERSION     XP_077502545.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646419.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..371
                     /product="acid phosphatase 1"
                     /calculated_mol_wt=42286
     Region          27..316
                     /region_name="HP"
                     /note="Histidine phosphatase domain found in a
                     functionally diverse set of proteins, mostly phosphatases;
                     contains a His residue which is phosphorylated during the
                     reaction; cl11399"
                     /db_xref="CDD:472174"
     Site            order(35..36,39,101,273..274)
                     /site_type="active"
                     /note="catalytic core [active]"
                     /db_xref="CDD:132717"
     CDS             1..371
                     /gene="Acph-1"
                     /coded_by="XM_077646419.1:177..1292"
                     /db_xref="GeneID:144113365"
ORIGIN      
        1 mklvcllvla agaaarsiqq ciepsslrql hvmfrhgdrt ptslypndpn spsdfpeglg
       61 hithkgkndq ynlgrylrtk yedfltydpn emrarssgre rclesiqtnl yglypprdkk
      121 vwnsevdwqp vpiqtmpvdl dgmlyedavc prddeeleri rhspegaevl rsnanlmrtl
      181 qdlsgkkmtd wvsvrdlldt ltiersrnlk ipdwalplwg nmtrvakytt ilnynstlhn
      241 rlraglmire ilrhsdavvn gnsnvklymy aahdvliaaf tsafgvfnql avpsstaiit
      301 elhedadgnf yiqmlfkndt trkpyrleip gcegfrcnlk tfkeiakpyv vedwrqecgl
      361 dplpggfdlf p
//
LOCUS       XP_077502546             152 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113366 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502546
VERSION     XP_077502546.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646420.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..152
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..152
                     /product="uncharacterized protein LOC144113366 isoform X1"
                     /calculated_mol_wt=16903
     CDS             1..152
                     /gene="LOC144113366"
                     /coded_by="XM_077646420.1:107..565"
                     /db_xref="GeneID:144113366"
ORIGIN      
        1 mshilikwve eakwdvypvk sladpaigir llteegaikq lkgsvvsvfw kegeqpakae
       61 llylgkqasl ekkraklaae apecvdrrre dsqpsagmst cnehpccrsa aqqlkeqqee
      121 isclrnenes lkrqleaaek iavqhskmvc sd
//
LOCUS       XP_077502547             138 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113366 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502547
VERSION     XP_077502547.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646421.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..138
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..138
                     /product="uncharacterized protein LOC144113366 isoform X2"
                     /calculated_mol_wt=15253
     Region          63..>120
                     /region_name="BEN"
                     /note="The BEN domain is found in diverse animal proteins;
                     smart01025"
                     /db_xref="CDD:214981"
     CDS             1..138
                     /gene="LOC144113366"
                     /coded_by="XM_077646421.1:548..964"
                     /db_xref="GeneID:144113366"
ORIGIN      
        1 msflflllav pakmvkrler mleskkepdq pvvpcakvdi gngvlvdrti lqmlerqcqs
       61 spgkfarall rnvfteeelr gkslygmknn vqkglpvkea ldpvrlsavi gytcdhfpgv
      121 nvaylknsla allarelk
//
LOCUS       XP_077502548             138 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113366 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502548
VERSION     XP_077502548.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646422.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..138
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..138
                     /product="uncharacterized protein LOC144113366 isoform X2"
                     /calculated_mol_wt=15253
     Region          63..>120
                     /region_name="BEN"
                     /note="The BEN domain is found in diverse animal proteins;
                     smart01025"
                     /db_xref="CDD:214981"
     CDS             1..138
                     /gene="LOC144113366"
                     /coded_by="XM_077646422.1:544..960"
                     /db_xref="GeneID:144113366"
ORIGIN      
        1 msflflllav pakmvkrler mleskkepdq pvvpcakvdi gngvlvdrti lqmlerqcqs
       61 spgkfarall rnvfteeelr gkslygmknn vqkglpvkea ldpvrlsavi gytcdhfpgv
      121 nvaylknsla allarelk
//
LOCUS       XP_077502549             922 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113370 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502549
VERSION     XP_077502549.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646423.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..922
                     /product="uncharacterized protein LOC144113370 isoform X1"
                     /calculated_mol_wt=105055
     Region          <292..335
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cl21498"
                     /db_xref="CDD:473887"
     Region          326..370
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cd00167"
                     /db_xref="CDD:238096"
     Site            order(327,358..359,361..362,364..366,368..370)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238096"
     Region          380..443
                     /region_name="Myb_DNA-bind_6"
                     /note="Myb-like DNA-binding domain; pfam13921"
                     /db_xref="CDD:372817"
     Site            order(380,413..414,416..417,419..421,423..425)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238096"
     Region          430..>539
                     /region_name="REB1"
                     /note="Myb superfamily proteins, including transcription
                     factors and mRNA splicing factors [Transcription / RNA
                     processing and modification / Cell division and chromosome
                     partitioning]; COG5147"
                     /db_xref="CDD:227476"
     Region          435..478
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cd00167"
                     /db_xref="CDD:238096"
     Site            order(435,465..466,468..469,471..473,475..477)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238096"
     CDS             1..922
                     /gene="LOC144113370"
                     /coded_by="XM_077646423.1:50..2818"
                     /db_xref="GeneID:144113370"
ORIGIN      
        1 meedslemdp gpsselqldl gpvsftvddy eteesdvdes elygfqdnad qgapadsysq
       61 feesadeklp gasetyafqd kavhgapads yshfeenvce kplpgsssae apsrqqeclk
      121 akpsyqelvh iniqelteal ernrklqkdl pmllthlavr rkkpkvrknw yhslvfhypy
      181 frdvngmpap pnedeltkra nkeldpymsp vvpwsanedr slvdavqsnl lqqnmeavqn
      241 rsevlaerll ttkdpkqhae lserkaqldk evcdmpdlsl vellesstrp vdwlrieakd
      301 mrsnrtaldc emhwhhlldv rlnqdpwtpt edsrlcelaa kydehcwdev aeelntgrsa
      361 yqcavryfrh havrfnsgpf saeedeqlkt lvsryrngks vnwfevahim nwrskkqlmn
      421 ryqrsldpqv lhghwaaeed mmllvavkly gcdnwskvat llpsrtnnqc rehflnffey
      481 qvvsgpyipc edqtlvelvd khgqgcwsav skdmpwrtpg svllrykrlk qlmekdevtv
      541 adlqkyyats gdkvardrrg mtsamdrryd vfrricrmit tstqrevalk lckgegqnld
      601 reaclrlyqr mrylflngrp lnselaqaka lnkeiaqyaq pgqrtiapnl gayerqewqa
      661 vknvlhdiyg lerppgdpev eevgtlpafe effcmsvlvv padyhvgssh lapllspnet
      721 tmatfgrlvd rfadgehads wlylcdgsaf pelcealdav nidsirctyc hsralsedel
      781 csfasrewap dlyqhacrrc eqlrdarkky dllcgrflsf flwpllvdeq nvidtvalpp
      841 cthpkraksy ytpkkvkkpw vkaawakrkr laaeaaaams gngsqeggqe erdgdtaiae
      901 ssadgavpsa swgessltng mt
//
LOCUS       XP_077502550             771 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113370 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502550
VERSION     XP_077502550.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646424.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..771
                     /product="uncharacterized protein LOC144113370 isoform X2"
                     /calculated_mol_wt=88336
     Region          <141..184
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cl21498"
                     /db_xref="CDD:473887"
     Region          176..237
                     /region_name="Myb_DNA-bind_6"
                     /note="Myb-like DNA-binding domain; pfam13921"
                     /db_xref="CDD:372817"
     Site            order(176,207..208,210..211,213..215,217..219)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238096"
     Region          229..292
                     /region_name="Myb_DNA-bind_6"
                     /note="Myb-like DNA-binding domain; pfam13921"
                     /db_xref="CDD:372817"
     Site            order(229,262..263,265..266,268..270,272..274)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238096"
     Region          279..>388
                     /region_name="REB1"
                     /note="Myb superfamily proteins, including transcription
                     factors and mRNA splicing factors [Transcription / RNA
                     processing and modification / Cell division and chromosome
                     partitioning]; COG5147"
                     /db_xref="CDD:227476"
     Region          284..327
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cd00167"
                     /db_xref="CDD:238096"
     Site            order(284,314..315,317..318,320..322,324..326)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238096"
     CDS             1..771
                     /gene="LOC144113370"
                     /coded_by="XM_077646424.1:231..2546"
                     /db_xref="GeneID:144113370"
ORIGIN      
        1 mllthlavrr kkpkvrknwy hslvfhypyf rdvngmpapp nedeltkran keldpymspv
       61 vpwsanedrs lvdavqsnll qqnmeavqnr sevlaerllt tkdpkqhael serkaqldke
      121 vcdmpdlslv ellesstrpv dwlrieakdm rsnrtaldce mhwhhlldvr lnqdpwtpte
      181 dsrlcelaak ydehcwdeva eelntgrsay qcavryfrhh avrfnsgpfs aeedeqlktl
      241 vsryrngksv nwfevahimn wrskkqlmnr yqrsldpqvl hghwaaeedm mllvavklyg
      301 cdnwskvatl lpsrtnnqcr ehflnffeyq vvsgpyipce dqtlvelvdk hgqgcwsavs
      361 kdmpwrtpgs vllrykrlkq lmekdevtva dlqkyyatsg dkvardrrgm tsamdrrydv
      421 frricrmitt stqrevalkl ckgegqnldr eaclrlyqrm rylflngrpl nselaqakal
      481 nkeiaqyaqp gqrtiapnlg ayerqewqav knvlhdiygl erppgdpeve evgtlpafee
      541 ffcmsvlvvp adyhvgsshl apllspnett matfgrlvdr fadgehadsw lylcdgsafp
      601 elcealdavn idsirctych sralsedelc sfasrewapd lyqhacrrce qlrdarkkyd
      661 llcgrflsff lwpllvdeqn vidtvalppc thpkraksyy tpkkvkkpwv kaawakrkrl
      721 aaeaaaamsg ngsqeggqee rdgdtaiaes sadgavpsas wgessltngm t
//
LOCUS       XP_077502551             180 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113374 [Amblyomma americanum].
ACCESSION   XP_077502551
VERSION     XP_077502551.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646425.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..180
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..180
                     /product="uncharacterized protein LOC144113374"
                     /calculated_mol_wt=19482
     CDS             1..180
                     /gene="LOC144113374"
                     /coded_by="XM_077646425.1:21..563"
                     /db_xref="GeneID:144113374"
ORIGIN      
        1 mlrvfrlnaa lvqrltplrv pgelrfpggr vhdvrvlglp alqrhgdvaa slkggvlgls
       61 gglgldellv qcqytagprr plqlagevdg rvtnltarmt lgvnvarata sldvfkfrni
      121 gnvqvtrltg vtppfnwlgv aiinlilqdq adrlrqklee kakkalnesl entripitin
//
LOCUS       XP_077502552             306 aa            linear   INV 25-NOV-2025
DEFINITION  putative rRNA-processing protein EBP2 [Amblyomma americanum].
ACCESSION   XP_077502552
VERSION     XP_077502552.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646426.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..306
                     /product="putative rRNA-processing protein EBP2"
                     /calculated_mol_wt=34756
     Region          38..295
                     /region_name="Ebp2"
                     /note="Eukaryotic rRNA processing protein EBP2; pfam05890"
                     /db_xref="CDD:461770"
     CDS             1..306
                     /gene="LOC144113378"
                     /coded_by="XM_077646426.1:173..1093"
                     /db_xref="GeneID:144113378"
ORIGIN      
        1 msdsesldtd eelqeslakg elkpglyaia phvkkefinn taalkqklae meldldwvet
       61 ltmvnglapl tpelseqfgd melqknrkgt vvmgsnedpv hhdfkremaf yrqaqaavle
      121 giprlhqlgv vtrrpddyfa qmaksdthmt kvreklltkk valersekar klrelkkfgk
      181 kvqvevmqqr qkekremmen lkkfkkgqgs ldflentgkq kgpkpaaagn qpgkgkrlsk
      241 sqlkrqykdk kygyggqrkr skfntasssa dvegfsrkkh nmapgagkkr pgkarrqnmk
      301 nkqrrk
//
LOCUS       XP_077502553             889 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113379 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502553
VERSION     XP_077502553.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646427.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..889
                     /product="uncharacterized protein LOC144113379 isoform X1"
                     /calculated_mol_wt=101613
     Region          <259..302
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cl21498"
                     /db_xref="CDD:473887"
     Region          294..355
                     /region_name="Myb_DNA-bind_6"
                     /note="Myb-like DNA-binding domain; pfam13921"
                     /db_xref="CDD:372817"
     Site            order(294,325..326,328..329,331..333,335..337)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238096"
     Region          347..410
                     /region_name="Myb_DNA-bind_6"
                     /note="Myb-like DNA-binding domain; pfam13921"
                     /db_xref="CDD:372817"
     Site            order(347,380..381,383..384,386..388,390..392)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238096"
     Region          397..>506
                     /region_name="REB1"
                     /note="Myb superfamily proteins, including transcription
                     factors and mRNA splicing factors [Transcription / RNA
                     processing and modification / Cell division and chromosome
                     partitioning]; COG5147"
                     /db_xref="CDD:227476"
     Region          402..445
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cd00167"
                     /db_xref="CDD:238096"
     Site            order(402,432..433,435..436,438..440,442..444)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238096"
     CDS             1..889
                     /gene="LOC144113379"
                     /coded_by="XM_077646427.1:78..2747"
                     /db_xref="GeneID:144113379"
ORIGIN      
        1 meedslemdp gpsselqldl gpvsftvddc eteesdvgas etyafqdkav hgapadsysh
       61 feenvcekpl pgsssaeaps rqqeclkakp syqelvhini qeltealern rklqkdlpml
      121 lthlavrrkk pkvrknwyhs mvfhypyfrd vngmpappne deltkranke ldpymspvvp
      181 wsadedrslv davqsnllqq nmeavqnrse vlaerllttk dpkqhaelse rkaqldkevc
      241 dmpdlslvel lesstrpvdw rrieakdmrs nrtaldcemh whhlldvrln qdpwtpteds
      301 rlcelaakyd khcwdevaee lntgrsayqc avryfrhhav rfnsgpfsae edeqlktlvs
      361 ryrngksvnw fevahimnwr skkqlmnryq rsldpqvlhg hwaaeedmml lvavklygcd
      421 nwskvatllp srtnnqcreh flnffeyqvv sgpytpcedq tlvelvdkhg qgcwsavskd
      481 mpwrtpgsvl lrykrlkqlm ekdevtvadl qkyyatsgdk vardrrgmts amdrrydvfr
      541 ricrmittst qrevalklck gegqnldrea clrlyqrmry lflngrplny elaqakalnk
      601 eiaqyaqpgq rtiapnlgay erqewqavkn vlhdiygler ppgdpeveev gtlpafeeff
      661 cmsvlvvpad yhvgsshlap llspnettma tfgrlvdrfa dgehadswly lcdgsafpel
      721 cealdavnid sirctychsr alsedelcsf asrewapdly qhacrrceql rdtrkkydll
      781 cgrflsfflw pllvdeqnvi dtvalppcth pkraksyytp kkvkkpwvka awakrkrlaa
      841 eaaaamsgng sqergqeerd gdtaiaessa dgavpsaswg essltngmt
//
LOCUS       XP_077502554             771 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113379 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502554
VERSION     XP_077502554.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646428.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..771
                     /product="uncharacterized protein LOC144113379 isoform X2"
                     /calculated_mol_wt=88590
     Region          <141..184
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cl21498"
                     /db_xref="CDD:473887"
     Region          176..237
                     /region_name="Myb_DNA-bind_6"
                     /note="Myb-like DNA-binding domain; pfam13921"
                     /db_xref="CDD:372817"
     Site            order(176,207..208,210..211,213..215,217..219)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238096"
     Region          229..292
                     /region_name="Myb_DNA-bind_6"
                     /note="Myb-like DNA-binding domain; pfam13921"
                     /db_xref="CDD:372817"
     Site            order(229,262..263,265..266,268..270,272..274)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238096"
     Region          279..>388
                     /region_name="REB1"
                     /note="Myb superfamily proteins, including transcription
                     factors and mRNA splicing factors [Transcription / RNA
                     processing and modification / Cell division and chromosome
                     partitioning]; COG5147"
                     /db_xref="CDD:227476"
     Region          284..327
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cd00167"
                     /db_xref="CDD:238096"
     Site            order(284,314..315,317..318,320..322,324..326)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238096"
     CDS             1..771
                     /gene="LOC144113379"
                     /coded_by="XM_077646428.1:223..2538"
                     /db_xref="GeneID:144113379"
ORIGIN      
        1 mllthlavrr kkpkvrknwy hsmvfhypyf rdvngmpapp nedeltkran keldpymspv
       61 vpwsadedrs lvdavqsnll qqnmeavqnr sevlaerllt tkdpkqhael serkaqldke
      121 vcdmpdlslv ellesstrpv dwrrieakdm rsnrtaldce mhwhhlldvr lnqdpwtpte
      181 dsrlcelaak ydkhcwdeva eelntgrsay qcavryfrhh avrfnsgpfs aeedeqlktl
      241 vsryrngksv nwfevahimn wrskkqlmnr yqrsldpqvl hghwaaeedm mllvavklyg
      301 cdnwskvatl lpsrtnnqcr ehflnffeyq vvsgpytpce dqtlvelvdk hgqgcwsavs
      361 kdmpwrtpgs vllrykrlkq lmekdevtva dlqkyyatsg dkvardrrgm tsamdrrydv
      421 frricrmitt stqrevalkl ckgegqnldr eaclrlyqrm rylflngrpl nyelaqakal
      481 nkeiaqyaqp gqrtiapnlg ayerqewqav knvlhdiygl erppgdpeve evgtlpafee
      541 ffcmsvlvvp adyhvgsshl apllspnett matfgrlvdr fadgehadsw lylcdgsafp
      601 elcealdavn idsirctych sralsedelc sfasrewapd lyqhacrrce qlrdtrkkyd
      661 llcgrflsff lwpllvdeqn vidtvalppc thpkraksyy tpkkvkkpwv kaawakrkrl
      721 aaeaaaamsg ngsqergqee rdgdtaiaes sadgavpsas wgessltngm t
//
LOCUS       XP_077502555             349 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113381 [Amblyomma americanum].
ACCESSION   XP_077502555
VERSION     XP_077502555.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646429.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 37% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..349
                     /product="uncharacterized protein LOC144113381"
                     /calculated_mol_wt=39579
     CDS             1..349
                     /gene="LOC144113381"
                     /coded_by="XM_077646429.1:1..1050"
                     /db_xref="GeneID:144113381"
ORIGIN      
        1 mdivkppgpl qltgnvrrnw lifkqklqlf ltatstatpr saavkaaill saagdealdi
       61 ynfsyakdes kddydtllkk fgtyfdeken evyerhvfrm rvqvpvvqpa rrvpfslrep
      121 lreelnrmek asiikrvdep tewfipgkdl lladmlsrap alravleste dvdvhavqvl
      181 ssigrrlrtt lpecsieker pvvkhrqssk gkllpplqqg dtvrvrdgrq wsrkamvlqk
      241 vaprshivrt dndrllrrnr qhllrtqehy nvtddsddea dihsepsrts spgtadgqge
      301 prdglatstq ltassaatgs smpaiprwtt rprlepkrlt ydhefrqis
//
LOCUS       XP_077502557             278 aa            linear   INV 25-NOV-2025
DEFINITION  transcription factor LBX2-like [Amblyomma americanum].
ACCESSION   XP_077502557
VERSION     XP_077502557.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646431.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..278
                     /product="transcription factor LBX2-like"
                     /calculated_mol_wt=30742
     Region          <159..276
                     /region_name="COG5576"
                     /note="Homeodomain-containing transcription factor
                     [Transcription]"
                     /db_xref="CDD:227863"
     Region          180..236
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..278
                     /gene="LOC144113390"
                     /coded_by="XM_077646431.1:45..881"
                     /db_xref="GeneID:144113390"
ORIGIN      
        1 madgagqqpd epaaspahea gsplrpardd eelldvvsta sldgadfsed eeyfrpikrl
       61 cmleapanps pkpltsffik dilshkparr asgivrpwdl esaspgrrrp rstdedsrse
      121 sdsadspaap qphsstspld alfemtskaf eglesgdraa dasrenggsf ngrqpvkkkr
      181 ksrtaftnhq ifelekrfly qkylspadrd eiaqslgltn aqvitwfqnr raklkrdmee
      241 lkkdmdaaki htihktlldn iqdlssfkkd ihktasgp
//
LOCUS       XP_077502558             309 aa            linear   INV 25-NOV-2025
DEFINITION  T-cell leukemia homeobox protein 3-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502558
VERSION     XP_077502558.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646432.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..309
                     /product="T-cell leukemia homeobox protein 3-like isoform
                     X1"
                     /calculated_mol_wt=33300
     Region          150..206
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..309
                     /gene="LOC144113391"
                     /coded_by="XM_077646432.1:24..953"
                     /db_xref="GeneID:144113391"
ORIGIN      
        1 msarmtspep tdgsrsgspa spvpsaegad hkprlsfsis rllgddserr pdtllaggps
       61 pplvsempal vaaaqlsplh lgangtvirv pahrpaplga afpsfpwigp ahpavkdrlq
      121 vpfglpsamp pvpprrighp yqnrtppkrk kprtsftrmq icelekrfhk qkylasaera
      181 alakqlkmtd aqvktwfqnr rtkwrkispl ssyaafpddm rqtaeereae rhaanrlmms
      241 lqaevvsksi yegardslcm snaslhalqs lqpwaseaan alhagaagdr gpaaaaylap
      301 ppsltsplc
//
LOCUS       XP_077502559             294 aa            linear   INV 25-NOV-2025
DEFINITION  T-cell leukemia homeobox protein 3-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502559
VERSION     XP_077502559.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646433.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..294
                     /product="T-cell leukemia homeobox protein 3-like isoform
                     X2"
                     /calculated_mol_wt=31676
     Region          150..206
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..294
                     /gene="LOC144113391"
                     /coded_by="XM_077646433.1:24..908"
                     /db_xref="GeneID:144113391"
ORIGIN      
        1 msarmtspep tdgsrsgspa spvpsaegad hkprlsfsis rllgddserr pdtllaggps
       61 pplvsempal vaaaqlsplh lgangtvirv pahrpaplga afpsfpwigp ahpavkdrlq
      121 vpfglpsamp pvpprrighp yqnrtppkrk kprtsftrmq icelekrfhk qkylasaera
      181 alakqlkmtd aqvktwfqnr rtkwrrqtae ereaerhaan rlmmslqaev vsksiyegar
      241 dslcmsnasl halqslqpwa seaanalhag aagdrgpaaa aylapppslt splc
//
LOCUS       XP_077502560             359 aa            linear   INV 25-NOV-2025
DEFINITION  GDP-mannose 4,6 dehydratase [Amblyomma americanum].
ACCESSION   XP_077502560
VERSION     XP_077502560.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646434.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..359
                     /product="GDP-mannose 4,6 dehydratase"
                     /calculated_mol_wt=40674
     Region          11..354
                     /region_name="Gmd"
                     /note="GDP-D-mannose dehydratase [Cell
                     wall/membrane/envelope biogenesis]; COG1089"
                     /db_xref="CDD:440706"
     CDS             1..359
                     /gene="Gmd"
                     /coded_by="XM_077646434.1:165..1244"
                     /db_xref="GeneID:144113392"
ORIGIN      
        1 matgderlnr kvalisgitg qdgsylaefl lakgyevhgi irrsssfntg riehlykdpr
       61 thqqgsmqlh ygdltdslcl vklirqtnpt eiynlgaqsh vkvsfdlsey tanvdalgtl
      121 rileairvcg ledkvkfyqa stselygkvq eipqkettpf yprspygvak lygywivvny
      181 reaykmfavn gilfnhespr rgetfvtrki srsvakihlg qlecfelgnl dskrdwghak
      241 dyveamwlml qekepndfvi atgeyhsvre fvevafkhvg rtivwegsgl gevgkekesg
      301 tvrvkvnpky frpaevdfll gdaskakekl gwqrrisfqe lvkemvdsdi almkknpsa
//
LOCUS       XP_077502561             273 aa            linear   INV 25-NOV-2025
DEFINITION  Rieske iron-sulfur protein [Amblyomma americanum].
ACCESSION   XP_077502561
VERSION     XP_077502561.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646435.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..273
                     /product="Rieske iron-sulfur protein"
                     /calculated_mol_wt=29505
     Region          2..76
                     /region_name="Ubiq-Cytc-red_N"
                     /note="Ubiquinol-cytochrome c reductase 8 kDa, N-terminal;
                     pfam09165"
                     /db_xref="CDD:462698"
     Region          79..144
                     /region_name="UCR_TM"
                     /note="Ubiquinol cytochrome reductase transmembrane
                     region; pfam02921"
                     /db_xref="CDD:460747"
     Region          150..273
                     /region_name="Rieske_cytochrome_bc1"
                     /note="Iron-sulfur protein (ISP) component of the bc(1)
                     complex family, Rieske domain; The Rieske domain is a
                     [2Fe-2S] cluster binding domain involved in electron
                     transfer. The bc(1) complex is a multisubunit enzyme found
                     in many different organisms including...; cd03470"
                     /db_xref="CDD:239552"
     Site            order(216,218..219,235,238,240)
                     /site_type="other"
                     /note="[2Fe-2S] cluster binding site [ion binding]"
                     /db_xref="CDD:239552"
     CDS             1..273
                     /gene="RFeSP"
                     /coded_by="XM_077646435.1:75..896"
                     /db_xref="GeneID:144113393"
ORIGIN      
        1 misvkgraan lanyvkassk tvasqvqpvt pfvelkneek rppppaklsh yalakslptr
       61 ellacsgsft apaqvrmaht dvkvpdfsay rkkssldpak ssrdvqvssk mqtylilgvg
      121 amggtyaaks lvtkfvmsls asadvlamak ievklsdipe gknatfkwrg kplfvrhrts
      181 deisregavd msslrdpqhd rertqkpewl viigvcthlg cvpianagdf ggyycpchgs
      241 hydasgrirk gpaplnlevp pyefttdstl lvg
//
LOCUS       XP_077502562             118 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113394 [Amblyomma americanum].
ACCESSION   XP_077502562
VERSION     XP_077502562.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646436.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..118
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..118
                     /product="uncharacterized protein LOC144113394"
                     /calculated_mol_wt=13861
     CDS             1..118
                     /gene="LOC144113394"
                     /coded_by="XM_077646436.1:2310..2666"
                     /db_xref="GeneID:144113394"
ORIGIN      
        1 mrrqkeawkg raqelqesel ekekeywksr aqelqddnif lkaqvaslqr cleskmfqie
       61 qgkarysqgm taetlkhwgv qksvsteeae ktltrtltek iqdllksklr kqikflkt
//
LOCUS       XP_077502563             413 aa            linear   INV 25-NOV-2025
DEFINITION  forkhead box C1-B-like [Amblyomma americanum].
ACCESSION   XP_077502563
VERSION     XP_077502563.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646437.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..413
                     /product="forkhead box C1-B-like"
                     /calculated_mol_wt=44799
     Region          80..170
                     /region_name="FH_FOXL1"
                     /note="Forkhead (FH) domain found in Forkhead box protein
                     L1 (FOXL1) and similar proteins; cd20027"
                     /db_xref="CDD:410801"
     Site            order(86,104..105,108,127..128,130..131,134,141,151..153,
                     155)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410801"
     CDS             1..413
                     /gene="LOC144113396"
                     /coded_by="XM_077646437.1:530..1771"
                     /db_xref="GeneID:144113396"
ORIGIN      
        1 mhslygdhhg qgyyrysgys tppaaggsga sspallygaa hgqeplgayp grmgrqpqyv
       61 aapyatgama iqqqapkdmv kppysyiali amaiqnapek kitlngiyqf imdrfpfyre
      121 nkqgwqnsir hnlslnecfv kvprddkkpg kgsywtldpd svnmfdngsy lrrrrrfkkk
      181 dstkdankde aqrktaeled kkpvikqpsn svggrtsges hqavtpktep vadscllasc
      241 kyearpltqp lgeqqgtlal dpslstfsvd slmtrdqaad dlshysrplf qaamtpcpsp
      301 atlsytcssp gvyadrgstg ddavavsgad alpagqqqhy agsrpawytm qqqlldpsea
      361 yaasngfrea ytgasaapgs nsttggqssy qvgfplnnay qrsytagydc try
//
LOCUS       XP_077502564             413 aa            linear   INV 25-NOV-2025
DEFINITION  forkhead box C1-B-like [Amblyomma americanum].
ACCESSION   XP_077502564
VERSION     XP_077502564.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646438.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..413
                     /product="forkhead box C1-B-like"
                     /calculated_mol_wt=44799
     Region          80..170
                     /region_name="FH_FOXL1"
                     /note="Forkhead (FH) domain found in Forkhead box protein
                     L1 (FOXL1) and similar proteins; cd20027"
                     /db_xref="CDD:410801"
     Site            order(86,104..105,108,127..128,130..131,134,141,151..153,
                     155)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410801"
     CDS             1..413
                     /gene="LOC144113396"
                     /coded_by="XM_077646438.1:562..1803"
                     /db_xref="GeneID:144113396"
ORIGIN      
        1 mhslygdhhg qgyyrysgys tppaaggsga sspallygaa hgqeplgayp grmgrqpqyv
       61 aapyatgama iqqqapkdmv kppysyiali amaiqnapek kitlngiyqf imdrfpfyre
      121 nkqgwqnsir hnlslnecfv kvprddkkpg kgsywtldpd svnmfdngsy lrrrrrfkkk
      181 dstkdankde aqrktaeled kkpvikqpsn svggrtsges hqavtpktep vadscllasc
      241 kyearpltqp lgeqqgtlal dpslstfsvd slmtrdqaad dlshysrplf qaamtpcpsp
      301 atlsytcssp gvyadrgstg ddavavsgad alpagqqqhy agsrpawytm qqqlldpsea
      361 yaasngfrea ytgasaapgs nsttggqssy qvgfplnnay qrsytagydc try
//
LOCUS       XP_077502565             472 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113399 [Amblyomma americanum].
ACCESSION   XP_077502565
VERSION     XP_077502565.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646439.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..472
                     /product="uncharacterized protein LOC144113399"
                     /calculated_mol_wt=52384
     Region          221..267
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          302..462
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..472
                     /gene="LOC144113399"
                     /coded_by="XM_077646439.1:142..1560"
                     /db_xref="GeneID:144113399"
ORIGIN      
        1 madgsewvpn ertricskhf vngekstias hpgyyptifp dayrvshvda tkqlaryerl
       61 hrrrrarass asvmpkqasa tvsekdtass teedtsetdp lnqepsnlev lcmavdaaqk
      121 descqtddia rcsgtfsvfl csargneact qvnhkivrda evqckpavvs rqsgrdsrta
      181 cftgydsvqa caaamkdlcg vspdiyalll simprsaert cdvpitdrlv lflmklklgi
      241 tysslavlfc vsrntvsrhf ksvlktlsia tqkwiyrppd hvirqtmpnc fkvhyqdcsm
      301 iidctevrte qpytvqqqrv lyshykggyt lkflvgitpc gavcfcskay ggrcsdsfit
      361 vdsgfldlvq pgdvvmadkg fpgikaglee vravlvmppf lhgqdqfsgs evhetyqiaq
      421 vrihveriiq rikmfnilnt ripteliavm sdvfhvccvl anlqppiiss eq
//
LOCUS       XP_077502566             451 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113400 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502566
VERSION     XP_077502566.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646440.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..451
                     /product="uncharacterized protein LOC144113400 isoform X1"
                     /calculated_mol_wt=50824
     Region          16..>96
                     /region_name="SWIM"
                     /note="Uncharacterized protein, contains SWIM-type Zn
                     finger domain [Function unknown]; COG4715"
                     /db_xref="CDD:443750"
     Region          261..441
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(334,368,381,383)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..451
                     /gene="LOC144113400"
                     /coded_by="XM_077646440.1:102..1457"
                     /db_xref="GeneID:144113400"
ORIGIN      
        1 msqvvlagfr pikgteeyfh pasltsgkkl ydaghvsfvt evdgvnvrak crsqqgkqvy
       61 dvslhinknd relqaglcsc rygaagsckh caavalhvnm hddisctser qrwgrpskka
      121 rvddkasiee lfgankhphv gggeppkpld psflikhfpd iqcpmsrilk aqnendesac
      181 ktvlqdiidk vlleeeiekl rsllqepkka lhslmvtakf pkilikkkey eeempaevaa
      241 yyaknikcls alhlcaltkg qannhrwhre rrlritcsqa hmilrtrkpp qdllktilhr
      301 rgfcseatsy gtfsnittsh vgltesyfsg mkmepvarkn feekvcadvl etglvvhtaq
      361 pwlcgspdgl fetakgvcll eikcpfsrrn rdiidvdqei tfvpyikykn gkltllashr
      421 yytqvqllmy vcnlnecffl filnnkasps l
//
LOCUS       XP_077502567             432 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113400 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502567
VERSION     XP_077502567.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646441.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..432
                     /product="uncharacterized protein LOC144113400 isoform X2"
                     /calculated_mol_wt=48794
     Region          16..>96
                     /region_name="SWIM"
                     /note="Uncharacterized protein, contains SWIM-type Zn
                     finger domain [Function unknown]; COG4715"
                     /db_xref="CDD:443750"
     Region          261..420
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(315,349,362,364)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..432
                     /gene="LOC144113400"
                     /coded_by="XM_077646441.1:101..1399"
                     /db_xref="GeneID:144113400"
ORIGIN      
        1 msqvvlagfr pikgteeyfh pasltsgkkl ydaghvsfvt evdgvnvrak crsqqgkqvy
       61 dvslhinknd relqaglcsc rygaagsckh caavalhvnm hddisctser qrwgrpskka
      121 rvddkasiee lfgankhphv gggeppkpld psflikhfpd iqcpmsrilk aqnendesac
      181 ktvlqdiidk vlleeeiekl rsllqepkka lhslmvtakf pkilikkkey eeempaevaa
      241 yyaknikcls alhlcaltkg qannhrwhre rrlritcsqa hmilrtrkpp qdllktilhr
      301 rgfcseatsy gmkmepvark nfeekvcadv letglvvhta qpwlcgspdg lfetakgvcl
      361 leikcpfsrr nrdiidvdqe itfvpyikyk ngkltllash ryytqvqllm yvcnlnecff
      421 lfilnnkasp sl
//
LOCUS       XP_077502568             336 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113401 [Amblyomma americanum].
ACCESSION   XP_077502568
VERSION     XP_077502568.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646442.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..336
                     /product="uncharacterized protein LOC144113401"
                     /calculated_mol_wt=36699
     Region          18..85
                     /region_name="RRM_hnRNPC_like"
                     /note="RNA recognition motif (RRM) found in heterogeneous
                     nuclear ribonucleoprotein C (hnRNP C)-related proteins;
                     cd12341"
                     /db_xref="CDD:409778"
     Region          <202..>225
                     /region_name="ZnB-Zn_SUZ12-like"
                     /note="zinc finger-binding (ZnB) and C2H2-type zinc finger
                     (Zn) domain found in suppressor of zeste 12 protein
                     (SUZ12), and similar proteins; cl45905"
                     /db_xref="CDD:459250"
     CDS             1..336
                     /gene="LOC144113401"
                     /coded_by="XM_077646442.1:404..1414"
                     /db_xref="GeneID:144113401"
ORIGIN      
        1 mtrvgnqtns tdpaavnsri fvgnlnthvv skedverlfk rygriigism hkgyafvqyt
       61 dtydarnavl gedgrtiagq ildvnmvsep kphqvnrkrq nlapdpfatp rqlgspvkra
      121 rteplsfrpt samgnkgvak kklhtyaepd ilicgncrev fpglqklldh krhrcrlrft
      181 crchvvglfp aesppgiplp vcaqclarcg swwelachle sahgmsiyhh pleaatnsdd
      241 hdpkssqsdr imespnndgg ceeaasvasd srsddwasiv vecanhgsng ahstsdltde
      301 gldkthddsp aapshdtfek gvdttdlqpi iaaeqt
//
LOCUS       XP_077502571             266 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113402 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502571
VERSION     XP_077502571.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646445.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..266
                     /product="uncharacterized protein LOC144113402 isoform X1"
                     /calculated_mol_wt=28720
     Region          <81..110
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..266
                     /gene="LOC144113402"
                     /coded_by="XM_077646445.1:2657..3457"
                     /db_xref="GeneID:144113402"
ORIGIN      
        1 mapppppdae etvdqeleee maveeekeel pcdtastqqq pavvpscsaq qhaggggapa
       61 aagdttqtss kpamcrmrcf ihaywsskhq pdlcgitgne eadelataah qlpprdlpla
      121 ledvravihe hlqrqhpdpr iaggeriasi ngcralsrsq ramilcarig cvwpgdrrvr
      181 hglatseacd gcgavetleh lllrctafad acrdmlvayr elgllpdslk tllwpqgsar
      241 trertmvslc afleqtglts clfcar
//
LOCUS       XP_077502572             266 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113402 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502572
VERSION     XP_077502572.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646446.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..266
                     /product="uncharacterized protein LOC144113402 isoform X1"
                     /calculated_mol_wt=28720
     Region          <81..110
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..266
                     /gene="LOC144113402"
                     /coded_by="XM_077646446.1:2653..3453"
                     /db_xref="GeneID:144113402"
ORIGIN      
        1 mapppppdae etvdqeleee maveeekeel pcdtastqqq pavvpscsaq qhaggggapa
       61 aagdttqtss kpamcrmrcf ihaywsskhq pdlcgitgne eadelataah qlpprdlpla
      121 ledvravihe hlqrqhpdpr iaggeriasi ngcralsrsq ramilcarig cvwpgdrrvr
      181 hglatseacd gcgavetleh lllrctafad acrdmlvayr elgllpdslk tllwpqgsar
      241 trertmvslc afleqtglts clfcar
//
LOCUS       XP_077502573             266 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113402 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502573
VERSION     XP_077502573.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646447.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..266
                     /product="uncharacterized protein LOC144113402 isoform X1"
                     /calculated_mol_wt=28720
     Region          <81..110
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..266
                     /gene="LOC144113402"
                     /coded_by="XM_077646447.1:1103..1903"
                     /db_xref="GeneID:144113402"
ORIGIN      
        1 mapppppdae etvdqeleee maveeekeel pcdtastqqq pavvpscsaq qhaggggapa
       61 aagdttqtss kpamcrmrcf ihaywsskhq pdlcgitgne eadelataah qlpprdlpla
      121 ledvravihe hlqrqhpdpr iaggeriasi ngcralsrsq ramilcarig cvwpgdrrvr
      181 hglatseacd gcgavetleh lllrctafad acrdmlvayr elgllpdslk tllwpqgsar
      241 trertmvslc afleqtglts clfcar
//
LOCUS       XP_077502574             266 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113402 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502574
VERSION     XP_077502574.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646448.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..266
                     /product="uncharacterized protein LOC144113402 isoform X1"
                     /calculated_mol_wt=28720
     Region          <81..110
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..266
                     /gene="LOC144113402"
                     /coded_by="XM_077646448.1:1969..2769"
                     /db_xref="GeneID:144113402"
ORIGIN      
        1 mapppppdae etvdqeleee maveeekeel pcdtastqqq pavvpscsaq qhaggggapa
       61 aagdttqtss kpamcrmrcf ihaywsskhq pdlcgitgne eadelataah qlpprdlpla
      121 ledvravihe hlqrqhpdpr iaggeriasi ngcralsrsq ramilcarig cvwpgdrrvr
      181 hglatseacd gcgavetleh lllrctafad acrdmlvayr elgllpdslk tllwpqgsar
      241 trertmvslc afleqtglts clfcar
//
LOCUS       XP_077502575             266 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113402 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502575
VERSION     XP_077502575.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646449.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..266
                     /product="uncharacterized protein LOC144113402 isoform X1"
                     /calculated_mol_wt=28720
     Region          <81..110
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..266
                     /gene="LOC144113402"
                     /coded_by="XM_077646449.1:419..1219"
                     /db_xref="GeneID:144113402"
ORIGIN      
        1 mapppppdae etvdqeleee maveeekeel pcdtastqqq pavvpscsaq qhaggggapa
       61 aagdttqtss kpamcrmrcf ihaywsskhq pdlcgitgne eadelataah qlpprdlpla
      121 ledvravihe hlqrqhpdpr iaggeriasi ngcralsrsq ramilcarig cvwpgdrrvr
      181 hglatseacd gcgavetleh lllrctafad acrdmlvayr elgllpdslk tllwpqgsar
      241 trertmvslc afleqtglts clfcar
//
LOCUS       XP_077502576             246 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113402 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502576
VERSION     XP_077502576.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646450.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..246
                     /product="uncharacterized protein LOC144113402 isoform X2"
                     /calculated_mol_wt=26514
     Region          <61..90
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..246
                     /gene="LOC144113402"
                     /coded_by="XM_077646450.1:2689..3429"
                     /db_xref="GeneID:144113402"
ORIGIN      
        1 maveeekeel pcdtastqqq pavvpscsaq qhaggggapa aagdttqtss kpamcrmrcf
       61 ihaywsskhq pdlcgitgne eadelataah qlpprdlpla ledvravihe hlqrqhpdpr
      121 iaggeriasi ngcralsrsq ramilcarig cvwpgdrrvr hglatseacd gcgavetleh
      181 lllrctafad acrdmlvayr elgllpdslk tllwpqgsar trertmvslc afleqtglts
      241 clfcar
//
LOCUS       XP_077502577             246 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113402 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502577
VERSION     XP_077502577.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646451.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..246
                     /product="uncharacterized protein LOC144113402 isoform X2"
                     /calculated_mol_wt=26514
     Region          <61..90
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..246
                     /gene="LOC144113402"
                     /coded_by="XM_077646451.1:2685..3425"
                     /db_xref="GeneID:144113402"
ORIGIN      
        1 maveeekeel pcdtastqqq pavvpscsaq qhaggggapa aagdttqtss kpamcrmrcf
       61 ihaywsskhq pdlcgitgne eadelataah qlpprdlpla ledvravihe hlqrqhpdpr
      121 iaggeriasi ngcralsrsq ramilcarig cvwpgdrrvr hglatseacd gcgavetleh
      181 lllrctafad acrdmlvayr elgllpdslk tllwpqgsar trertmvslc afleqtglts
      241 clfcar
//
LOCUS       XP_077502578             246 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113402 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502578
VERSION     XP_077502578.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646452.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..246
                     /product="uncharacterized protein LOC144113402 isoform X2"
                     /calculated_mol_wt=26514
     Region          <61..90
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..246
                     /gene="LOC144113402"
                     /coded_by="XM_077646452.1:2609..3349"
                     /db_xref="GeneID:144113402"
ORIGIN      
        1 maveeekeel pcdtastqqq pavvpscsaq qhaggggapa aagdttqtss kpamcrmrcf
       61 ihaywsskhq pdlcgitgne eadelataah qlpprdlpla ledvravihe hlqrqhpdpr
      121 iaggeriasi ngcralsrsq ramilcarig cvwpgdrrvr hglatseacd gcgavetleh
      181 lllrctafad acrdmlvayr elgllpdslk tllwpqgsar trertmvslc afleqtglts
      241 clfcar
//
LOCUS       XP_077502579             246 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113402 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502579
VERSION     XP_077502579.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646453.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..246
                     /product="uncharacterized protein LOC144113402 isoform X2"
                     /calculated_mol_wt=26514
     Region          <61..90
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..246
                     /gene="LOC144113402"
                     /coded_by="XM_077646453.1:1921..2661"
                     /db_xref="GeneID:144113402"
ORIGIN      
        1 maveeekeel pcdtastqqq pavvpscsaq qhaggggapa aagdttqtss kpamcrmrcf
       61 ihaywsskhq pdlcgitgne eadelataah qlpprdlpla ledvravihe hlqrqhpdpr
      121 iaggeriasi ngcralsrsq ramilcarig cvwpgdrrvr hglatseacd gcgavetleh
      181 lllrctafad acrdmlvayr elgllpdslk tllwpqgsar trertmvslc afleqtglts
      241 clfcar
//
LOCUS       XP_077502580             200 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113402 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502580
VERSION     XP_077502580.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646454.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..200
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..200
                     /product="uncharacterized protein LOC144113402 isoform X3"
                     /calculated_mol_wt=22000
     CDS             1..200
                     /gene="LOC144113402"
                     /coded_by="XM_077646454.1:407..1009"
                     /db_xref="GeneID:144113402"
ORIGIN      
        1 magrwhlekd lfvvrlfhwl slsclqqlss qaadwcvara plpsgrrpps srvgercgtw
       61 qnakectage dgvppsrenl gnlgefgnvn lvdtllmlvr rqgrrtvemc inltfciklg
      121 qgqacrawpt trsvfaycct iskrhhstpy dfsgcirgtg ggtlpgltgr vcklqspsil
      181 kgvavamfst ssqrrrwtrs
//
LOCUS       XP_077502581             198 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113402 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077502581
VERSION     XP_077502581.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646455.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..198
                     /product="uncharacterized protein LOC144113402 isoform X4"
                     /calculated_mol_wt=21577
     CDS             1..198
                     /gene="LOC144113402"
                     /coded_by="XM_077646455.1:407..1003"
                     /db_xref="GeneID:144113402"
ORIGIN      
        1 magrwhlekd lfvvrlfhwl slsclqqlss qaadwcvara plpsgrrpps srvgercgtw
       61 qnakectage dgvppsrenl gnlgefgnvn lvdtllmlvr rqgrrtvemc inltfciklg
      121 qgqacrawpt trsvfaycct iskrhhstpy dfsgcirgtg ggtlpgltgr vcklqspsil
      181 kgvavamfst ssqnqphv
//
LOCUS       XP_077502582             198 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113402 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077502582
VERSION     XP_077502582.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646456.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..198
                     /product="uncharacterized protein LOC144113402 isoform X4"
                     /calculated_mol_wt=21577
     CDS             1..198
                     /gene="LOC144113402"
                     /coded_by="XM_077646456.1:407..1003"
                     /db_xref="GeneID:144113402"
ORIGIN      
        1 magrwhlekd lfvvrlfhwl slsclqqlss qaadwcvara plpsgrrpps srvgercgtw
       61 qnakectage dgvppsrenl gnlgefgnvn lvdtllmlvr rqgrrtvemc inltfciklg
      121 qgqacrawpt trsvfaycct iskrhhstpy dfsgcirgtg ggtlpgltgr vcklqspsil
      181 kgvavamfst ssqnqphv
//
LOCUS       XP_077502583             198 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113402 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077502583
VERSION     XP_077502583.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646457.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..198
                     /product="uncharacterized protein LOC144113402 isoform X4"
                     /calculated_mol_wt=21577
     CDS             1..198
                     /gene="LOC144113402"
                     /coded_by="XM_077646457.1:407..1003"
                     /db_xref="GeneID:144113402"
ORIGIN      
        1 magrwhlekd lfvvrlfhwl slsclqqlss qaadwcvara plpsgrrpps srvgercgtw
       61 qnakectage dgvppsrenl gnlgefgnvn lvdtllmlvr rqgrrtvemc inltfciklg
      121 qgqacrawpt trsvfaycct iskrhhstpy dfsgcirgtg ggtlpgltgr vcklqspsil
      181 kgvavamfst ssqnqphv
//
LOCUS       XP_077502584             159 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113402 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077502584
VERSION     XP_077502584.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646458.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..159
                     /product="uncharacterized protein LOC144113402 isoform X5"
                     /calculated_mol_wt=17754
     CDS             1..159
                     /gene="LOC144113402"
                     /coded_by="XM_077646458.1:407..886"
                     /db_xref="GeneID:144113402"
ORIGIN      
        1 magrwhlekd lfvvrlfhwl slsclqqlss qaadwcvara plpsgrrpps srvgercgtw
       61 qnakectage dgvppsrenl gnlgefgnvn lvdtllmlvr rqgrrtvemc inltfciklg
      121 qgqacrawpt trsvfaycct iskrhhstpy dfsgcirki
//
LOCUS       XP_077502586             159 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113402 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077502586
VERSION     XP_077502586.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646460.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..159
                     /product="uncharacterized protein LOC144113402 isoform X5"
                     /calculated_mol_wt=17754
     CDS             1..159
                     /gene="LOC144113402"
                     /coded_by="XM_077646460.1:407..886"
                     /db_xref="GeneID:144113402"
ORIGIN      
        1 magrwhlekd lfvvrlfhwl slsclqqlss qaadwcvara plpsgrrpps srvgercgtw
       61 qnakectage dgvppsrenl gnlgefgnvn lvdtllmlvr rqgrrtvemc inltfciklg
      121 qgqacrawpt trsvfaycct iskrhhstpy dfsgcirki
//
LOCUS       XP_077502587             369 aa            linear   INV 25-NOV-2025
DEFINITION  exonuclease V-like [Amblyomma americanum].
ACCESSION   XP_077502587
VERSION     XP_077502587.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646461.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..369
                     /product="exonuclease V-like"
                     /calculated_mol_wt=41722
     Region          33..298
                     /region_name="Exo5"
                     /note="Exonuclease V - a 5' deoxyribonuclease; pfam09810"
                     /db_xref="CDD:430843"
     CDS             1..369
                     /gene="LOC144113404"
                     /coded_by="XM_077646461.1:187..1296"
                     /db_xref="GeneID:144113404"
ORIGIN      
        1 mddsgaanaw ddeegddlia klhfdgltds rpplqkyrng riyvsdlssq vyceqkllyt
       61 ligvprlkem gfdveeaerp qvargshihl arqlevqdvv avdtktredn iavlfvnllh
      121 avrsllsgap ivrevpvfgr vldgdifvng vvdeircdge tlqidivelk tiagtrlpna
      181 svrrphrlqv mlyrhllasl ilgkvdvkdi ekgfhvnldv qlsspvlell pskerhmnsl
      241 gllvpfvlta iqalpvpsql tveyfsqktn atlcfqgvey deswlvdtlq qlfpfwtgsw
      301 lcstwlfcsr egldyrrrpd naelleyrgl rcfkdhratr hlslrersta vhgprlnids
      361 rcdinvtll
//
LOCUS       XP_077502588             458 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113405 [Amblyomma americanum].
ACCESSION   XP_077502588
VERSION     XP_077502588.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646462.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (DAPXPI010021904.1) annotated
            using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 368 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..458
                     /product="uncharacterized protein LOC144113405"
                     /calculated_mol_wt=47166
     Region          <57..>220
                     /region_name="PRK12323"
                     /note="DNA polymerase III subunit gamma/tau; cl46901"
                     /db_xref="CDD:481241"
     Region          218..274
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..458
                     /gene="LOC144113405"
                     /coded_by="XM_077646462.1:27..1403"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 368 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144113405"
ORIGIN      
        1 mesgaaaspy plhswhphvy aqppksptph siahilgird darndqplnl scpgrtqpsp
       61 pglaaspras ahdaktapsp lpalgtpslp hadaqlalpk aarslpshwt pghagllqrg
      121 psglrivrvl gamggaqgvq rpapvpkgak rkkepggrea epsaapsptp avtnpggspg
      181 ipsgaspgaq qnpaanrarl sespvngges gdkaarkkka rttftgrqif elerqfeikk
      241 ylssseraem akllnvtetq vkiwfqnrrt kwkkqdgisn aeaaelkige kaakakgtaa
      301 akaageqplg ltktagassp llpaagsnss salsvnhhtf sgtssakpsp cgvsdlsnls
      361 ssgdrtddss qhfppssdld kmengtaals sdgadaacrd spgadqrpfr eaaeltsrag
      421 gaavakqhad gfapspsrss vlvcdetdkf qdsgqveq
//
LOCUS       XP_077502589            4948 aa            linear   INV 25-NOV-2025
DEFINITION  obscurin isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502589
VERSION     XP_077502589.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646463.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..4948
                     /product="obscurin isoform X1"
                     /calculated_mol_wt=548963
     Region          228..288
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     Site            order(233,235,238,244,262..263,282,284..285)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212690"
     Region          312..489
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          496..600
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          707..796
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          724..728
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          737..741
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          762..766
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          776..781
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          789..792
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          821..911
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          838..842
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          851..855
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          877..881
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          891..896
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          904..907
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <935..1003
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          942..946
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          970..973
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          983..988
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          996..999
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1015..1105
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1032..1036
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1045..1049
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1071..1075
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1085..1090
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1098..1101
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1109..1192
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1126..1130
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1139..1143
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1164..1169
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1179..1184
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1192..1195
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1203..1294
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1220..1224
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1233..1237
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1260..1264
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1274..1279
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1287..1290
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1298..1390
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1316..1320
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1329..1333
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1356..1360
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1370..1375
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1383..1386
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1394..1483
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1411..1415
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1424..1428
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1451..1455
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1465..1470
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1478..1481
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1489..1578
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1506..1510
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1519..1523
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1544..1548
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1558..1563
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1571..1574
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1582..1671
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1599..1603
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1612..1616
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1634..1641
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1651..1656
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1664..1667
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1675..1764
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1692..1696
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1705..1709
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1730..1734
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1744..1749
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1757..1760
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1773..1862
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1790..1794
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1803..1807
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1829..1832
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1842..1847
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1855..1858
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1873..1956
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1884..1888
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1897..1901
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1924..1928
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1937..1942
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1950..1953
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1962..2049
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1979..1983
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1992..1996
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2017..2021
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2031..2036
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2044..2047
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2063..2146
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2074..2078
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2087..2091
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2113..2117
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2127..2132
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2140..2143
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2158..2248
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2175..2179
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2189..2193
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2215..2219
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2229..2234
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2242..2245
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2258..2347
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2275..2279
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2288..2292
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2314..2318
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2328..2333
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2341..2344
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2358..2445
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2375..2379
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2389..2393
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2415..2419
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2429..2434
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2442..2445
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2460..2550
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2477..2481
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2491..2495
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2517..2521
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2531..2536
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2544..2547
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2572..2657
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2587..2591
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2601..2605
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2627..2631
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2641..2646
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2654..2657
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2672..2759
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2689..2693
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2703..2707
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2729..2733
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2743..2748
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2756..2759
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2778..2864
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2791..2795
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2805..2809
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2831..2835
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2845..2850
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2858..2861
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2876..2966
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2893..2897
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2906..2910
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2932..2936
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2946..2951
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2959..2962
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2974..3064
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2991..2995
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3004..3008
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3030..3034
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3044..3049
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3057..3060
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3068..3159
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3085..3089
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3098..3102
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3125..3129
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3139..3144
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3152..3155
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3174..3260
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3188..3192
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3201..3205
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3226..3230
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3240..3245
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3253..3256
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3266..3355
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3296..3300
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3321..3325
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3335..3340
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3362..3452
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3379..3383
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3392..3396
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3419..3422
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3432..3437
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3445..3448
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3571..3662
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(3571,3637,3652)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(3653..3654,3656..3657)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          3714..3718
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          <3720..3786
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3727..3731
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3753..3757
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3767..3772
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3791..3882
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3808..3812
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3821..3825
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3847..3851
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3861..3866
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3874..3877
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3907..4163
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(3916..3919,3922,3924,3937,3939,3967,3983..3986,4029,
                     4033..4034,4036,4048..4049)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     Region          4391..4481
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4408..4412
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4421..4425
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4447..4451
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4461..4466
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4474..4477
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4485..4577
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(4485,4550,4565)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(4566..4567,4569..4570)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          4629..4886
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(4638..4641,4644,4646,4659,4661,4689,4705..4708,4751,
                     4755..4756,4758,4770..4771)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..4948
                     /gene="Obsc"
                     /coded_by="XM_077646463.1:229..15075"
                     /db_xref="GeneID:144113406"
ORIGIN      
        1 msysrssysr tskfseglsd erssrtsars sslmdsdiss smtssrrmmr ttsygdeggs
       61 sfssstvrrs srlsaleddd igsriqsssv rtsrlssrnd ddfgstaisr rpfaissgds
      121 fessrkssls ssvlgesvds sasralrrls kdddkesfas krskltskss fdvdessasv
      181 rysstkitss iessdlggas ysaskasysy rdsagsatep eagviggiye aildykplss
      241 deeglplkeg qevevidtsk prkwrvrtrs srtgisqegw vpccylekkq gavieeqvap
      301 kdresyakre avvkelvete edfsrdmqrv vnnylkemen pimpkelrdq kdvlfsnfkd
      361 icdfhnteli kgvqfnasep aklgvtflrl erdfdkhvky cedfpraqel lqsgplkeyf
      421 ddfsakindd ktlsdhlklp iqrindyqll lkelikytar lhedytdlqr aldfmqaipq
      481 rasdlqyina iegyhgnihn wgrvikhgwf diidaknreq lrylflfkgr lcitevkkir
      541 anrniylvkn vlktseseiv etgdddatlk ilqkdssgqd vpitlrakdi eqkkewarel
      601 qasrvasevv eemqlddtse rqqekssysr ttslsestav pvspdpasas mpraeifeas
      661 keatsaaths vrsgsvtsid eiqslnsfae desvyltask kdatgkpqfr kalrgltcsq
      721 ndntflecev sspekptiqw lkdniplkks srvqlseeeg kyklyindas sedsgmytii
      781 asnsegttsc saplyvkpal svakpdsrpt spggtilpha pvfkvklkdt ellegtsvrf
      841 elvvrgcpep evtvykngkk lveddrvrvm feskevfelv idhisfedmg kytcvavnse
      901 gkdessgtit vtkdkilfqg ltaddsaaeg sgpltpppks plfrwfkdgq efeaserfqv
      961 tfddiedtls lvfqhvtpdd aglytcvast ssgkiscsae ltvqgavikk apeaptiaas
     1021 lsdvevnega samlelkvkg ypkpkvtwkh egkvieaggr yrflfedees mtlvikntkk
     1081 tdagiysvva enesgtante crltvnstpt fkkelkdvsv mtddvlkldv evqgtpapdv
     1141 kwykdgqavv eddrvkitqp aedahqliik rakvedsgny scvisntmgt qtgfsavsvn
     1201 appkflqklk dyeasetenv tfrvkisgnp kpnvswkkdg kplkpdghry qtleetenvh
     1261 cliiddvhqe daaqytveia neygvetdsa altitckpkf kkplsdvecc egetnikitv
     1321 evegspapkv kwslndslic ednsfkfesv eesgkytlvi keaksdmtge mvceaanvkg
     1381 kdkcsgkftv kskpkivkgl ddvnvevgqs atfsvkvvna kdakvkwlkd dcevtidnkn
     1441 vyakeeadas ftlviksvtp edagqytfki knelgedsst grlstqkkpe fkkklqdqei
     1501 seedvaeftv vvtgepkpnv kwlhgkreik endvyeitsd gdtytlkvkk pnkelageyt
     1561 ceaqntcgke scsasltihs kpeiieglkd iegevgdaaf ftakiagspt ptvvwkkdde
     1621 eitveatike ipqgksykve fdeltlllag nyecvatnky gsastrgklt vqskpkfekk
     1681 penqcvysge ecvfeakvsg fptpnvtwsr dgetieesak ftisshngth rltvkdakkd
     1741 decfytctak nnlgrvsqqa eltiklpdda eaptfirrmt dtvavigdkv rfevrvagkp
     1801 tpkvtwykdg eelfegrrve ikedgdthti tlkdlvmrde akysckavne kgeatdkaql
     1861 tvkepvapai kglkdqeast gstvkfqati tgipapdviw kkdgkelsps hkitissdve
     1921 tesytlvihd vdddfgqytc kasnsagete adamltlkge ppvfikeled vkvtineeav
     1981 fhsrikgnpt psatwllngs iltesedfsi tqhgdsytlk itkvtshdvg evvckaknta
     2041 gqasskagls lketaptvak slpskvrved geplkleakv pghpepdvqw lkdgepivps
     2101 dhvkltqepd gtvalevdei rpddaghyav vanndkgkaa tesdvetkpk gddsvkspaf
     2161 seglkdatlt egkpghleak lakgsapsdv qwlkdgepvk psdhvkmvek pdgtialdid
     2221 kvtpsdaghy svmaendegk aasdadvktk psdlegkpvf eeglkpttlt egepgrleak
     2281 mpaeaaapve wtkdgapvkp tdhvklveep dgtvalelde vnpddaghya vkatndkgst
     2341 qseadvktqp kgaepskprf ekglqpatlp egkparleak vapdtlpikv ewlkdgkpvk
     2401 lddrvraeqk pdgtlalvid nvkpqdaghy avvasndagk tpseadvdti pkkaakdqkp
     2461 ifekglapte ltegkpgkle aklqpgdkpl diqwlkdgkp irpsdrvkpe ekpdgtvcls
     2521 iddvkpedag rysvvaknpd getaseadvt tkpdktaakp ekaaakpkkp afekdlepat
     2581 ltegkparle aklapgsnpt nvkwlkdgrp irpssrvkpe ekpdgtlalv iddvrpedag
     2641 nysvvaenda geaeseadik tqpekyrkqr kpafekelap ttltegkpar leaklepdsn
     2701 ptsvkwmkdg rplrpsdrik meekpdgtla lliddvrped agnysvvaan dageaesead
     2761 iktqpdkyrk qrrpafekel qpatlpegkp arleaklepd snptsikwlk dgrpirpsdr
     2821 irteerpdgt lalviddvrp edaghysvva andageaasd advktkpear vpgekpefvi
     2881 elknadlleg qplhlvgkvk sdspfttewl kdgkkvepsl rlalsqepdg tvllsiehat
     2941 pddagkyvcv aknpegkaes sstvkvaelp kyepeiveel kpavftegep gkleakvsgd
     3001 pmpdvkwikd gkelpedsri rsttspggdv altidpvkpe dagkydlvaa ndegecrtsa
     3061 pvtvnhppkf vkplepvevv egyparlett lagqptpdte wqkdgkplip dgkniketkt
     3121 psgdvaleip katpddagvy tckaknplge ketkapltva gsdtgdkaee kptvsplddv
     3181 evmesepftl eakitgkptp evewfyggkp ipraadlett fdgrkarlss khslpshagr
     3241 yecratnsag kasskatvgv tpmskpyftk rltdleaspn eplklvtkvk gypepeitwr
     3301 fngkviepsm rytmskegev ctltvpwpes kdcgvyecla knpvgedkcs gritirekge
     3361 ppsfvkrlen lripegdtat ftariagtpl pevkwfkdge eleltrrhkt elepngnlrl
     3421 tirdckpedf geyrasiynp ygsdtcmatl rvespyddgk rrpstdkyps rdipektdvr
     3481 rgrspsprqy pevngyrapp prderrrepy sgpyshelpp tsplpgrdfd rpdydpksrh
     3541 pphpryddld rpydritrpd lepepyyagv pdpltdkpyi rrmtdrdltl gwkpslptrs
     3601 ripvtytvem aplpdgnwsp yksgvkdtql dvrglepfkd yrfrvrvgnk hglsdpspyv
     3661 tahrsrlggs ppvpkefkpk dfdiphppld kpgeapvfvr heedvmygir ghpvsiefwv
     3721 ygnpqpditw tfkgtkvemg grynsmqdrn gqvtlfisrm tednvgaytc tavnehgqat
     3781 ktiylelaee pvftkrlept timlrrggel qcrvigkpyp kikwfkdwqp icntsrvnii
     3841 weapdvctla lngsiaadag lyscsasnia gtascsamls ieesesefdw rtyrqpkiir
     3901 pksthledyy nlgdelgrgt qgvtyhaver stgrsfgakt mhgrgqfkdw mkseldmmnq
     3961 lchprllrlw dafetknsms litdfcgggp lldsiisqdr vsehqianyi kqileglnym
     4021 haqniahlgl tlgdalvare nsddikigdf slstrlprgk vviqeyghpe fvapeiankk
     4081 paslasdmws vgvisyills gispflglnd retlrnvqdg kinflhdgfa kvsdeardfi
     4141 skllvfepse rmdvktaleh pwlkiterpd rgeglsnidr lkeyqrhwrn wyanascrnt
     4201 yrrrpmescf thpskmiypp devytppmsp dreisrskvk sapiegpgkg pndrmdisse
     4261 ssyqsgpdty llqlrdvdfp lrirqylrvg asrspslaas lrerhwggfd inergtrnvy
     4321 pqvvvrerrk fvdvmdeeid dekkglgtrt lplrlqrevg slgyahhqme hlkheawkdk
     4381 tsreksigma pffrekmedc viqendevvf rclavgnptp qyswfrndsi liessrvqit
     4441 qdpdgrselr lkpgkaydvg lfkcaarnsh gvalcharlk igekpsrpep pickqcsshq
     4501 ayvqwyspky dgnsrifcfc leyketlgae wtkvsdsitq efwvipdlkp atsymfrvsa
     4561 knkygwsets lssdpcttad egaaqkiqls rehkhqeeif egeyeletrv pldygqetsp
     4621 vqlesgqlnd lytfsselav grfgavvsgt skklssglal kvvlintdpe tsahreyevl
     4681 kslrhervic lktasvhddv vvlgleklcg mdvltflsmr htynedmvsr iikqvldgle
     4741 ylhfrsicyi elqpdnvvlt dahscniklv dfgsanfvpr tgkilpldts acleylapev
     4801 lkghevchat dvwgvgvlty illsghspfl gvneedtktn ilyvryhfdk lykeasteat
     4861 rflmqlfkrt pekrpaisec lenkwmlpse fmlrkrensv flshklqefa anfhlkkkna
     4921 staerlskli glqlsrssls siegsgda
//
LOCUS       XP_077502590            4931 aa            linear   INV 25-NOV-2025
DEFINITION  obscurin isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502590
VERSION     XP_077502590.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646464.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..4931
                     /product="obscurin isoform X2"
                     /calculated_mol_wt=547036
     Region          228..288
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     Site            order(233,235,238,244,262..263,282,284..285)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212690"
     Region          312..489
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          496..600
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          690..779
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          707..711
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          720..724
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          745..749
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          759..764
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          772..775
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          804..894
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          821..825
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          834..838
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          860..864
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          874..879
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          887..890
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <918..986
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          925..929
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          953..956
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          966..971
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          979..982
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          998..1088
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1015..1019
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1028..1032
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1054..1058
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1068..1073
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1081..1084
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1092..1175
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1109..1113
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1122..1126
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1147..1152
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1162..1167
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1175..1178
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1186..1277
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1203..1207
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1216..1220
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1243..1247
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1257..1262
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1270..1273
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1281..1373
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1299..1303
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1312..1316
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1339..1343
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1353..1358
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1366..1369
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1377..1466
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1394..1398
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1407..1411
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1434..1438
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1448..1453
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1461..1464
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1472..1561
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1489..1493
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1502..1506
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1527..1531
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1541..1546
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1554..1557
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1565..1654
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1582..1586
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1595..1599
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1617..1624
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1634..1639
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1647..1650
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1658..1747
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1675..1679
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1688..1692
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1713..1717
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1727..1732
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1740..1743
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1756..1845
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1773..1777
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1786..1790
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1812..1815
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1825..1830
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1838..1841
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1856..1939
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1867..1871
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1880..1884
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1907..1911
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1920..1925
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1933..1936
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1945..2032
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1962..1966
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1975..1979
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2000..2004
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2014..2019
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2027..2030
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2046..2129
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2057..2061
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2070..2074
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2096..2100
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2110..2115
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2123..2126
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2141..2231
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2158..2162
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2172..2176
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2198..2202
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2212..2217
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2225..2228
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2241..2330
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2258..2262
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2271..2275
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2297..2301
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2311..2316
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2324..2327
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2341..2428
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2358..2362
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2372..2376
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2398..2402
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2412..2417
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2425..2428
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2443..2533
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2460..2464
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2474..2478
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2500..2504
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2514..2519
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2527..2530
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2555..2640
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2570..2574
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2584..2588
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2610..2614
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2624..2629
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2637..2640
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2655..2742
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2672..2676
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2686..2690
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2712..2716
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2726..2731
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2739..2742
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2761..2847
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2774..2778
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2788..2792
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2814..2818
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2828..2833
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2841..2844
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2859..2949
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2876..2880
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2889..2893
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2915..2919
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2929..2934
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2942..2945
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2957..3047
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2974..2978
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2987..2991
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3013..3017
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3027..3032
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3040..3043
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3051..3142
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3068..3072
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3081..3085
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3108..3112
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3122..3127
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3135..3138
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3157..3243
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3171..3175
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3184..3188
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3209..3213
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3223..3228
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3236..3239
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3249..3338
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3279..3283
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3304..3308
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3318..3323
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3345..3435
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3362..3366
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3375..3379
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3402..3405
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3415..3420
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3428..3431
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3554..3645
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(3554,3620,3635)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(3636..3637,3639..3640)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          3697..3701
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          <3703..3769
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3710..3714
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3736..3740
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3750..3755
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3774..3865
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3791..3795
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3804..3808
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3830..3834
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3844..3849
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3857..3860
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3890..4146
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(3899..3902,3905,3907,3920,3922,3950,3966..3969,4012,
                     4016..4017,4019,4031..4032)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     Region          4374..4464
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4391..4395
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4404..4408
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4430..4434
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4444..4449
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4457..4460
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4468..4560
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(4468,4533,4548)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(4549..4550,4552..4553)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          4612..4869
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(4621..4624,4627,4629,4642,4644,4672,4688..4691,4734,
                     4738..4739,4741,4753..4754)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..4931
                     /gene="Obsc"
                     /coded_by="XM_077646464.1:229..15024"
                     /db_xref="GeneID:144113406"
ORIGIN      
        1 msysrssysr tskfseglsd erssrtsars sslmdsdiss smtssrrmmr ttsygdeggs
       61 sfssstvrrs srlsaleddd igsriqsssv rtsrlssrnd ddfgstaisr rpfaissgds
      121 fessrkssls ssvlgesvds sasralrrls kdddkesfas krskltskss fdvdessasv
      181 rysstkitss iessdlggas ysaskasysy rdsagsatep eagviggiye aildykplss
      241 deeglplkeg qevevidtsk prkwrvrtrs srtgisqegw vpccylekkq gavieeqvap
      301 kdresyakre avvkelvete edfsrdmqrv vnnylkemen pimpkelrdq kdvlfsnfkd
      361 icdfhnteli kgvqfnasep aklgvtflrl erdfdkhvky cedfpraqel lqsgplkeyf
      421 ddfsakindd ktlsdhlklp iqrindyqll lkelikytar lhedytdlqr aldfmqaipq
      481 rasdlqyina iegyhgnihn wgrvikhgwf diidaknreq lrylflfkgr lcitevkkir
      541 anrniylvkn vlktseseiv etgdddatlk ilqkdssgqd vpitlrakdi eqkkewarel
      601 qasrvasevv eemqlddtse rqqekssysr ttslsestav pvspdpasas mpraeifeas
      661 keatsaaths vrsgsvtsid askkdatgkp qfrkalrglt csqndntfle cevsspekpt
      721 iqwlkdnipl kkssrvqlse eegkyklyin dassedsgmy tiiasnsegt tscsaplyvk
      781 palsvakpds rptspggtil phapvfkvkl kdtellegts vrfelvvrgc pepevtvykn
      841 gkklveddrv rvmfeskevf elvidhisfe dmgkytcvav nsegkdessg titvtkdkil
      901 fqgltaddsa aegsgpltpp pksplfrwfk dgqefeaser fqvtfddied tlslvfqhvt
      961 pddaglytcv astssgkisc saeltvqgav ikkapeapti aaslsdvevn egasamlelk
     1021 vkgypkpkvt wkhegkviea ggryrflfed eesmtlvikn tkktdagiys vvaenesgta
     1081 ntecrltvns tptfkkelkd vsvmtddvlk ldvevqgtpa pdvkwykdgq avveddrvki
     1141 tqpaedahql iikrakveds gnyscvisnt mgtqtgfsav svnappkflq klkdyeaset
     1201 envtfrvkis gnpkpnvswk kdgkplkpdg hryqtleete nvhcliiddv hqedaaqytv
     1261 eianeygvet dsaaltitck pkfkkplsdv eccegetnik itvevegspa pkvkwslnds
     1321 licednsfkf esveesgkyt lvikeaksdm tgemvceaan vkgkdkcsgk ftvkskpkiv
     1381 kglddvnvev gqsatfsvkv vnakdakvkw lkddcevtid nknvyakeea dasftlviks
     1441 vtpedagqyt fkiknelged sstgrlstqk kpefkkklqd qeiseedvae ftvvvtgepk
     1501 pnvkwlhgkr eikendvyei tsdgdtytlk vkkpnkelag eytceaqntc gkescsaslt
     1561 ihskpeiieg lkdiegevgd aafftakiag sptptvvwkk ddeeitveat ikeipqgksy
     1621 kvefdeltll lagnyecvat nkygsastrg kltvqskpkf ekkpenqcvy sgeecvfeak
     1681 vsgfptpnvt wsrdgetiee sakftisshn gthrltvkda kkddecfytc taknnlgrvs
     1741 qqaeltiklp ddaeaptfir rmtdtvavig dkvrfevrva gkptpkvtwy kdgeelfegr
     1801 rveikedgdt htitlkdlvm rdeakyscka vnekgeatdk aqltvkepva paikglkdqe
     1861 astgstvkfq atitgipapd viwkkdgkel spshkitiss dvetesytlv ihdvdddfgq
     1921 ytckasnsag eteadamltl kgeppvfike ledvkvtine eavfhsrikg nptpsatwll
     1981 ngsiltesed fsitqhgdsy tlkitkvtsh dvgevvckak ntagqasska glslketapt
     2041 vakslpskvr vedgeplkle akvpghpepd vqwlkdgepi vpsdhvkltq epdgtvalev
     2101 deirpddagh yavvanndkg kaatesdvet kpkgddsvks pafseglkda tltegkpghl
     2161 eaklakgsap sdvqwlkdge pvkpsdhvkm vekpdgtial didkvtpsda ghysvmaend
     2221 egkaasdadv ktkpsdlegk pvfeeglkpt tltegepgrl eakmpaeaaa pvewtkdgap
     2281 vkptdhvklv eepdgtvale ldevnpddag hyavkatndk gstqseadvk tqpkgaepsk
     2341 prfekglqpa tlpegkparl eakvapdtlp ikvewlkdgk pvklddrvra eqkpdgtlal
     2401 vidnvkpqda ghyavvasnd agktpseadv dtipkkaakd qkpifekgla pteltegkpg
     2461 kleaklqpgd kpldiqwlkd gkpirpsdrv kpeekpdgtv clsiddvkpe dagrysvvak
     2521 npdgetasea dvttkpdkta akpekaaakp kkpafekdle patltegkpa rleaklapgs
     2581 nptnvkwlkd grpirpssrv kpeekpdgtl alviddvrpe dagnysvvae ndageaesea
     2641 diktqpekyr kqrkpafeke lapttltegk parleaklep dsnptsvkwm kdgrplrpsd
     2701 rikmeekpdg tlalliddvr pedagnysvv aandageaes eadiktqpdk yrkqrrpafe
     2761 kelqpatlpe gkparleakl epdsnptsik wlkdgrpirp sdrirteerp dgtlalvidd
     2821 vrpedaghys vvaandagea asdadvktkp earvpgekpe fvielknadl legqplhlvg
     2881 kvksdspftt ewlkdgkkve pslrlalsqe pdgtvllsie hatpddagky vcvaknpegk
     2941 aessstvkva elpkyepeiv eelkpavfte gepgkleakv sgdpmpdvkw ikdgkelped
     3001 srirsttspg gdvaltidpv kpedagkydl vaandegecr tsapvtvnhp pkfvkplepv
     3061 evvegyparl ettlagqptp dtewqkdgkp lipdgknike tktpsgdval eipkatpdda
     3121 gvytckaknp lgeketkapl tvagsdtgdk aeekptvspl ddvevmesep ftleakitgk
     3181 ptpevewfyg gkpipraadl ettfdgrkar lsskhslpsh agryecratn sagkasskat
     3241 vgvtpmskpy ftkrltdlea spneplklvt kvkgypepei twrfngkvie psmrytmske
     3301 gevctltvpw peskdcgvye claknpvged kcsgritire kgeppsfvkr lenlripegd
     3361 tatftariag tplpevkwfk dgeeleltrr hktelepngn lrltirdckp edfgeyrasi
     3421 ynpygsdtcm atlrvespyd dgkrrpstdk ypsrdipekt dvrrgrspsp rqypevngyr
     3481 appprderrr epysgpyshe lpptsplpgr dfdrpdydpk srhpphpryd dldrpydrit
     3541 rpdlepepyy agvpdpltdk pyirrmtdrd ltlgwkpslp trsripvtyt vemaplpdgn
     3601 wspyksgvkd tqldvrglep fkdyrfrvrv gnkhglsdps pyvtahrsrl ggsppvpkef
     3661 kpkdfdiphp pldkpgeapv fvrheedvmy girghpvsie fwvygnpqpd itwtfkgtkv
     3721 emggrynsmq drngqvtlfi srmtednvga ytctavnehg qatktiylel aeepvftkrl
     3781 epttimlrrg gelqcrvigk pypkikwfkd wqpicntsrv niiweapdvc tlalngsiaa
     3841 daglyscsas niagtascsa mlsieesese fdwrtyrqpk iirpksthle dyynlgdelg
     3901 rgtqgvtyha verstgrsfg aktmhgrgqf kdwmkseldm mnqlchprll rlwdafetkn
     3961 smslitdfcg ggplldsiis qdrvsehqia nyikqilegl nymhaqniah lgltlgdalv
     4021 arensddiki gdfslstrlp rgkvviqeyg hpefvapeia nkkpaslasd mwsvgvisyi
     4081 llsgispflg lndretlrnv qdgkinflhd gfakvsdear dfiskllvfe psermdvkta
     4141 lehpwlkite rpdrgeglsn idrlkeyqrh wrnwyanasc rntyrrrpme scfthpskmi
     4201 yppdevytpp mspdreisrs kvksapiegp gkgpndrmdi ssessyqsgp dtyllqlrdv
     4261 dfplrirqyl rvgasrspsl aaslrerhwg gfdinergtr nvypqvvvre rrkfvdvmde
     4321 eiddekkglg trtlplrlqr evgslgyahh qmehlkheaw kdktsreksi gmapffrekm
     4381 edcviqende vvfrclavgn ptpqyswfrn dsiliessrv qitqdpdgrs elrlkpgkay
     4441 dvglfkcaar nshgvalcha rlkigekpsr peppickqcs shqayvqwys pkydgnsrif
     4501 cfcleyketl gaewtkvsds itqefwvipd lkpatsymfr vsaknkygws etslssdpct
     4561 tadegaaqki qlsrehkhqe eifegeyele trvpldygqe tspvqlesgq lndlytfsse
     4621 lavgrfgavv sgtskklssg lalkvvlint dpetsahrey evlkslrher viclktasvh
     4681 ddvvvlglek lcgmdvltfl smrhtynedm vsriikqvld gleylhfrsi cyielqpdnv
     4741 vltdahscni klvdfgsanf vprtgkilpl dtsacleyla pevlkghevc hatdvwgvgv
     4801 ltyillsghs pflgvneedt ktnilyvryh fdklykeast eatrflmqlf krtpekrpai
     4861 seclenkwml psefmlrkre nsvflshklq efaanfhlkk knastaerls kliglqlsrs
     4921 slssiegsgd a
//
LOCUS       XP_077502591            4902 aa            linear   INV 25-NOV-2025
DEFINITION  obscurin isoform X3 [Amblyomma americanum].
ACCESSION   XP_077502591
VERSION     XP_077502591.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646465.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..4902
                     /product="obscurin isoform X3"
                     /calculated_mol_wt=543383
     Region          228..288
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     Site            order(233,235,238,244,262..263,282,284..285)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212690"
     Region          312..489
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          661..750
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          678..682
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          691..695
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          716..720
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          730..735
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          743..746
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          775..865
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          792..796
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          805..809
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          831..835
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          845..850
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          858..861
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <889..957
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          896..900
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          924..927
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          937..942
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          950..953
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          969..1059
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          986..990
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          999..1003
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1025..1029
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1039..1044
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1052..1055
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1063..1146
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1080..1084
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1093..1097
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1118..1123
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1133..1138
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1146..1149
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1157..1248
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1174..1178
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1187..1191
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1214..1218
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1228..1233
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1241..1244
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1252..1344
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1270..1274
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1283..1287
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1310..1314
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1324..1329
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1337..1340
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1348..1437
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1365..1369
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1378..1382
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1405..1409
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1419..1424
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1432..1435
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1443..1532
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1460..1464
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1473..1477
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1498..1502
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1512..1517
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1525..1528
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1536..1625
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1553..1557
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1566..1570
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1588..1595
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1605..1610
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1618..1621
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1629..1718
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1646..1650
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1659..1663
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1684..1688
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1698..1703
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1711..1714
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1727..1816
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1744..1748
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1757..1761
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1783..1786
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1796..1801
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1809..1812
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1827..1910
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1838..1842
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1851..1855
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1878..1882
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1891..1896
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1904..1907
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1916..2003
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1933..1937
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1946..1950
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1971..1975
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1985..1990
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1998..2001
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2017..2100
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2028..2032
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2041..2045
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2067..2071
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2081..2086
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2094..2097
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2112..2202
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2129..2133
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2143..2147
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2169..2173
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2183..2188
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2196..2199
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2212..2301
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2229..2233
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2242..2246
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2268..2272
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2282..2287
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2295..2298
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2312..2399
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2329..2333
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2343..2347
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2369..2373
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2383..2388
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2396..2399
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2414..2504
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2431..2435
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2445..2449
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2471..2475
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2485..2490
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2498..2501
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2526..2611
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2541..2545
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2555..2559
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2581..2585
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2595..2600
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2608..2611
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2626..2713
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2643..2647
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2657..2661
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2683..2687
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2697..2702
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2710..2713
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2732..2818
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2745..2749
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2759..2763
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2785..2789
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2799..2804
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2812..2815
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2830..2920
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2847..2851
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2860..2864
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2886..2890
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2900..2905
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2913..2916
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2928..3018
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2945..2949
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2958..2962
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2984..2988
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2998..3003
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3011..3014
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3022..3113
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3039..3043
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3052..3056
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3079..3083
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3093..3098
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3106..3109
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3128..3214
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3142..3146
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3155..3159
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3180..3184
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3194..3199
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3207..3210
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3220..3309
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3250..3254
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3275..3279
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3289..3294
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3316..3406
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3333..3337
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3346..3350
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3373..3376
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3386..3391
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3399..3402
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3525..3616
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(3525,3591,3606)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(3607..3608,3610..3611)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          3668..3672
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          <3674..3740
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3681..3685
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3707..3711
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3721..3726
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3745..3836
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3762..3766
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3775..3779
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3801..3805
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3815..3820
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3828..3831
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3861..4117
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(3870..3873,3876,3878,3891,3893,3921,3937..3940,3983,
                     3987..3988,3990,4002..4003)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     Region          4345..4435
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4362..4366
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4375..4379
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4401..4405
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4415..4420
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4428..4431
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4439..4531
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(4439,4504,4519)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(4520..4521,4523..4524)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          4583..4840
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(4592..4595,4598,4600,4613,4615,4643,4659..4662,4705,
                     4709..4710,4712,4724..4725)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..4902
                     /gene="Obsc"
                     /coded_by="XM_077646465.1:229..14937"
                     /db_xref="GeneID:144113406"
ORIGIN      
        1 msysrssysr tskfseglsd erssrtsars sslmdsdiss smtssrrmmr ttsygdeggs
       61 sfssstvrrs srlsaleddd igsriqsssv rtsrlssrnd ddfgstaisr rpfaissgds
      121 fessrkssls ssvlgesvds sasralrrls kdddkesfas krskltskss fdvdessasv
      181 rysstkitss iessdlggas ysaskasysy rdsagsatep eagviggiye aildykplss
      241 deeglplkeg qevevidtsk prkwrvrtrs srtgisqegw vpccylekkq gavieeqvap
      301 kdresyakre avvkelvete edfsrdmqrv vnnylkemen pimpkelrdq kdvlfsnfkd
      361 icdfhnteli kgvqfnasep aklgvtflrl erdfdkhvky cedfpraqel lqsgplkeyf
      421 ddfsakindd ktlsdhlklp iqrindyqll lkelikytar lhedytdlqr aldfmqaipq
      481 rasdlqyina iegyhgnihn wgrvikhtse seivetgddd atlkilqkds sgqdvpitlr
      541 akdieqkkew arelqasrva sevveemqld dtserqqeks sysrttslse stavpvspdp
      601 asasmpraei feaskeatsa athsvrsgsv tsideiqsln sfaedesvyl taskkdatgk
      661 pqfrkalrgl tcsqndntfl ecevsspekp tiqwlkdnip lkkssrvqls eeegkyklyi
      721 ndassedsgm ytiiasnseg ttscsaplyv kpalsvakpd srptspggti lphapvfkvk
      781 lkdtellegt svrfelvvrg cpepevtvyk ngkklveddr vrvmfeskev felvidhisf
      841 edmgkytcva vnsegkdess gtitvtkdki lfqgltadds aaegsgpltp ppksplfrwf
      901 kdgqefease rfqvtfddie dtlslvfqhv tpddaglytc vastssgkis csaeltvqga
      961 vikkapeapt iaaslsdvev negasamlel kvkgypkpkv twkhegkvie aggryrflfe
     1021 deesmtlvik ntkktdagiy svvaenesgt antecrltvn stptfkkelk dvsvmtddvl
     1081 kldvevqgtp apdvkwykdg qavveddrvk itqpaedahq liikrakved sgnyscvisn
     1141 tmgtqtgfsa vsvnappkfl qklkdyease tenvtfrvki sgnpkpnvsw kkdgkplkpd
     1201 ghryqtleet envhcliidd vhqedaaqyt veianeygve tdsaaltitc kpkfkkplsd
     1261 veccegetni kitvevegsp apkvkwslnd slicednsfk fesveesgky tlvikeaksd
     1321 mtgemvceaa nvkgkdkcsg kftvkskpki vkglddvnve vgqsatfsvk vvnakdakvk
     1381 wlkddcevti dnknvyakee adasftlvik svtpedagqy tfkiknelge dsstgrlstq
     1441 kkpefkkklq dqeiseedva eftvvvtgep kpnvkwlhgk reikendvye itsdgdtytl
     1501 kvkkpnkela geytceaqnt cgkescsasl tihskpeiie glkdiegevg daafftakia
     1561 gsptptvvwk kddeeitvea tikeipqgks ykvefdeltl llagnyecva tnkygsastr
     1621 gkltvqskpk fekkpenqcv ysgeecvfea kvsgfptpnv twsrdgetie esakftissh
     1681 ngthrltvkd akkddecfyt ctaknnlgrv sqqaeltikl pddaeaptfi rrmtdtvavi
     1741 gdkvrfevrv agkptpkvtw ykdgeelfeg rrveikedgd thtitlkdlv mrdeakysck
     1801 avnekgeatd kaqltvkepv apaikglkdq eastgstvkf qatitgipap dviwkkdgke
     1861 lspshkitis sdvetesytl vihdvdddfg qytckasnsa geteadamlt lkgeppvfik
     1921 eledvkvtin eeavfhsrik gnptpsatwl lngsiltese dfsitqhgds ytlkitkvts
     1981 hdvgevvcka kntagqassk aglslketap tvakslpskv rvedgeplkl eakvpghpep
     2041 dvqwlkdgep ivpsdhvklt qepdgtvale vdeirpddag hyavvanndk gkaatesdve
     2101 tkpkgddsvk spafseglkd atltegkpgh leaklakgsa psdvqwlkdg epvkpsdhvk
     2161 mvekpdgtia ldidkvtpsd aghysvmaen degkaasdad vktkpsdleg kpvfeeglkp
     2221 ttltegepgr leakmpaeaa apvewtkdga pvkptdhvkl veepdgtval eldevnpdda
     2281 ghyavkatnd kgstqseadv ktqpkgaeps kprfekglqp atlpegkpar leakvapdtl
     2341 pikvewlkdg kpvklddrvr aeqkpdgtla lvidnvkpqd aghyavvasn dagktpsead
     2401 vdtipkkaak dqkpifekgl apteltegkp gkleaklqpg dkpldiqwlk dgkpirpsdr
     2461 vkpeekpdgt vclsiddvkp edagrysvva knpdgetase advttkpdkt aakpekaaak
     2521 pkkpafekdl epatltegkp arleaklapg snptnvkwlk dgrpirpssr vkpeekpdgt
     2581 lalviddvrp edagnysvva endageaese adiktqpeky rkqrkpafek elapttlteg
     2641 kparleakle pdsnptsvkw mkdgrplrps drikmeekpd gtlalliddv rpedagnysv
     2701 vaandageae seadiktqpd kyrkqrrpaf ekelqpatlp egkparleak lepdsnptsi
     2761 kwlkdgrpir psdrirteer pdgtlalvid dvrpedaghy svvaandage aasdadvktk
     2821 pearvpgekp efvielknad llegqplhlv gkvksdspft tewlkdgkkv epslrlalsq
     2881 epdgtvllsi ehatpddagk yvcvaknpeg kaessstvkv aelpkyepei veelkpavft
     2941 egepgkleak vsgdpmpdvk wikdgkelpe dsrirsttsp ggdvaltidp vkpedagkyd
     3001 lvaandegec rtsapvtvnh ppkfvkplep vevvegypar lettlagqpt pdtewqkdgk
     3061 plipdgknik etktpsgdva leipkatpdd agvytckakn plgeketkap ltvagsdtgd
     3121 kaeekptvsp lddvevmese pftleakitg kptpevewfy ggkpipraad lettfdgrka
     3181 rlsskhslps hagryecrat nsagkasska tvgvtpmskp yftkrltdle aspneplklv
     3241 tkvkgypepe itwrfngkvi epsmrytmsk egevctltvp wpeskdcgvy eclaknpvge
     3301 dkcsgritir ekgeppsfvk rlenlripeg dtatftaria gtplpevkwf kdgeeleltr
     3361 rhktelepng nlrltirdck pedfgeyras iynpygsdtc matlrvespy ddgkrrpstd
     3421 kypsrdipek tdvrrgrsps prqypevngy rappprderr repysgpysh elpptsplpg
     3481 rdfdrpdydp ksrhpphpry ddldrpydri trpdlepepy yagvpdpltd kpyirrmtdr
     3541 dltlgwkpsl ptrsripvty tvemaplpdg nwspyksgvk dtqldvrgle pfkdyrfrvr
     3601 vgnkhglsdp spyvtahrsr lggsppvpke fkpkdfdiph ppldkpgeap vfvrheedvm
     3661 ygirghpvsi efwvygnpqp ditwtfkgtk vemggrynsm qdrngqvtlf isrmtednvg
     3721 aytctavneh gqatktiyle laeepvftkr lepttimlrr ggelqcrvig kpypkikwfk
     3781 dwqpicntsr vniiweapdv ctlalngsia adaglyscsa sniagtascs amlsieeses
     3841 efdwrtyrqp kiirpksthl edyynlgdel grgtqgvtyh averstgrsf gaktmhgrgq
     3901 fkdwmkseld mmnqlchprl lrlwdafetk nsmslitdfc gggplldsii sqdrvsehqi
     3961 anyikqileg lnymhaqnia hlgltlgdal varensddik igdfslstrl prgkvviqey
     4021 ghpefvapei ankkpaslas dmwsvgvisy illsgispfl glndretlrn vqdgkinflh
     4081 dgfakvsdea rdfiskllvf epsermdvkt alehpwlkit erpdrgegls nidrlkeyqr
     4141 hwrnwyanas crntyrrrpm escfthpskm iyppdevytp pmspdreisr skvksapieg
     4201 pgkgpndrmd issessyqsg pdtyllqlrd vdfplrirqy lrvgasrsps laaslrerhw
     4261 ggfdinergt rnvypqvvvr errkfvdvmd eeiddekkgl gtrtlplrlq revgslgyah
     4321 hqmehlkhea wkdktsreks igmapffrek medcviqend evvfrclavg nptpqyswfr
     4381 ndsiliessr vqitqdpdgr selrlkpgka ydvglfkcaa rnshgvalch arlkigekps
     4441 rpeppickqc sshqayvqwy spkydgnsri fcfcleyket lgaewtkvsd sitqefwvip
     4501 dlkpatsymf rvsaknkygw setslssdpc ttadegaaqk iqlsrehkhq eeifegeyel
     4561 etrvpldygq etspvqlesg qlndlytfss elavgrfgav vsgtskklss glalkvvlin
     4621 tdpetsahre yevlkslrhe rviclktasv hddvvvlgle klcgmdvltf lsmrhtyned
     4681 mvsriikqvl dgleylhfrs icyielqpdn vvltdahscn iklvdfgsan fvprtgkilp
     4741 ldtsacleyl apevlkghev chatdvwgvg vltyillsgh spflgvneed tktnilyvry
     4801 hfdklykeas teatrflmql fkrtpekrpa iseclenkwm lpsefmlrkr ensvflshkl
     4861 qefaanfhlk kknastaerl skliglqlsr sslssiegsg da
//
LOCUS       XP_077502592            4885 aa            linear   INV 25-NOV-2025
DEFINITION  obscurin isoform X4 [Amblyomma americanum].
ACCESSION   XP_077502592
VERSION     XP_077502592.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646466.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..4885
                     /product="obscurin isoform X4"
                     /calculated_mol_wt=541456
     Region          228..288
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     Site            order(233,235,238,244,262..263,282,284..285)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212690"
     Region          312..489
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          644..733
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          661..665
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          674..678
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          699..703
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          713..718
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          726..729
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          758..848
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          775..779
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          788..792
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          814..818
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          828..833
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          841..844
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <872..940
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          879..883
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          907..910
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          920..925
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          933..936
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          952..1042
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          969..973
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          982..986
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1008..1012
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1022..1027
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1035..1038
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1046..1129
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1063..1067
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1076..1080
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1101..1106
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1116..1121
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1129..1132
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1140..1231
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1157..1161
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1170..1174
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1197..1201
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1211..1216
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1224..1227
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1235..1327
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1253..1257
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1266..1270
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1293..1297
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1307..1312
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1320..1323
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1331..1420
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1348..1352
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1361..1365
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1388..1392
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1402..1407
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1415..1418
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1426..1515
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1443..1447
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1456..1460
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1481..1485
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1495..1500
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1508..1511
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1519..1608
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1536..1540
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1549..1553
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1571..1578
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1588..1593
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1601..1604
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1612..1701
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1629..1633
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1642..1646
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1667..1671
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1681..1686
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1694..1697
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1710..1799
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1727..1731
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1740..1744
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1766..1769
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1779..1784
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1792..1795
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1810..1893
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1821..1825
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1834..1838
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1861..1865
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1874..1879
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1887..1890
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1899..1986
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1916..1920
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1929..1933
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1954..1958
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1968..1973
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1981..1984
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2000..2083
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2011..2015
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2024..2028
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2050..2054
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2064..2069
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2077..2080
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2095..2185
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2112..2116
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2126..2130
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2152..2156
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2166..2171
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2179..2182
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2195..2284
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2212..2216
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2225..2229
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2251..2255
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2265..2270
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2278..2281
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2295..2382
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2312..2316
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2326..2330
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2352..2356
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2366..2371
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2379..2382
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2397..2487
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2414..2418
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2428..2432
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2454..2458
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2468..2473
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2481..2484
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2509..2594
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2524..2528
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2538..2542
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2564..2568
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2578..2583
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2591..2594
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2609..2696
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2626..2630
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2640..2644
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2666..2670
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2680..2685
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2693..2696
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2715..2801
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2728..2732
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2742..2746
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2768..2772
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2782..2787
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2795..2798
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2813..2903
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2830..2834
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2843..2847
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2869..2873
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2883..2888
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2896..2899
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2911..3001
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2928..2932
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2941..2945
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2967..2971
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2981..2986
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2994..2997
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3005..3096
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3022..3026
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3035..3039
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3062..3066
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3076..3081
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3089..3092
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3111..3197
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3125..3129
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3138..3142
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3163..3167
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3177..3182
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3190..3193
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3203..3292
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3233..3237
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3258..3262
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3272..3277
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3299..3389
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3316..3320
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3329..3333
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3356..3359
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3369..3374
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3382..3385
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3508..3599
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(3508,3574,3589)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(3590..3591,3593..3594)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          3651..3655
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          <3657..3723
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3664..3668
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3690..3694
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3704..3709
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3728..3819
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3745..3749
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3758..3762
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3784..3788
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3798..3803
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3811..3814
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3844..4100
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(3853..3856,3859,3861,3874,3876,3904,3920..3923,3966,
                     3970..3971,3973,3985..3986)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     Region          4328..4418
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4345..4349
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4358..4362
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4384..4388
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4398..4403
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4411..4414
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4422..4514
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(4422,4487,4502)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(4503..4504,4506..4507)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          4566..4823
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(4575..4578,4581,4583,4596,4598,4626,4642..4645,4688,
                     4692..4693,4695,4707..4708)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..4885
                     /gene="Obsc"
                     /coded_by="XM_077646466.1:229..14886"
                     /db_xref="GeneID:144113406"
ORIGIN      
        1 msysrssysr tskfseglsd erssrtsars sslmdsdiss smtssrrmmr ttsygdeggs
       61 sfssstvrrs srlsaleddd igsriqsssv rtsrlssrnd ddfgstaisr rpfaissgds
      121 fessrkssls ssvlgesvds sasralrrls kdddkesfas krskltskss fdvdessasv
      181 rysstkitss iessdlggas ysaskasysy rdsagsatep eagviggiye aildykplss
      241 deeglplkeg qevevidtsk prkwrvrtrs srtgisqegw vpccylekkq gavieeqvap
      301 kdresyakre avvkelvete edfsrdmqrv vnnylkemen pimpkelrdq kdvlfsnfkd
      361 icdfhnteli kgvqfnasep aklgvtflrl erdfdkhvky cedfpraqel lqsgplkeyf
      421 ddfsakindd ktlsdhlklp iqrindyqll lkelikytar lhedytdlqr aldfmqaipq
      481 rasdlqyina iegyhgnihn wgrvikhtse seivetgddd atlkilqkds sgqdvpitlr
      541 akdieqkkew arelqasrva sevveemqld dtserqqeks sysrttslse stavpvspdp
      601 asasmpraei feaskeatsa athsvrsgsv tsidaskkda tgkpqfrkal rgltcsqndn
      661 tflecevssp ekptiqwlkd niplkkssrv qlseeegkyk lyindassed sgmytiiasn
      721 segttscsap lyvkpalsva kpdsrptspg gtilphapvf kvklkdtell egtsvrfelv
      781 vrgcpepevt vykngkklve ddrvrvmfes kevfelvidh isfedmgkyt cvavnsegkd
      841 essgtitvtk dkilfqglta ddsaaegsgp ltpppksplf rwfkdgqefe aserfqvtfd
      901 diedtlslvf qhvtpddagl ytcvastssg kiscsaeltv qgavikkape aptiaaslsd
      961 vevnegasam lelkvkgypk pkvtwkhegk vieaggryrf lfedeesmtl vikntkktda
     1021 giysvvaene sgtantecrl tvnstptfkk elkdvsvmtd dvlkldvevq gtpapdvkwy
     1081 kdgqavvedd rvkitqpaed ahqliikrak vedsgnyscv isntmgtqtg fsavsvnapp
     1141 kflqklkdye asetenvtfr vkisgnpkpn vswkkdgkpl kpdghryqtl eetenvhcli
     1201 iddvhqedaa qytveianey gvetdsaalt itckpkfkkp lsdveccege tnikitveve
     1261 gspapkvkws lndslicedn sfkfesvees gkytlvikea ksdmtgemvc eaanvkgkdk
     1321 csgkftvksk pkivkglddv nvevgqsatf svkvvnakda kvkwlkddce vtidnknvya
     1381 keeadasftl viksvtpeda gqytfkikne lgedsstgrl stqkkpefkk klqdqeisee
     1441 dvaeftvvvt gepkpnvkwl hgkreikend vyeitsdgdt ytlkvkkpnk elageytcea
     1501 qntcgkescs asltihskpe iieglkdieg evgdaaffta kiagsptptv vwkkddeeit
     1561 veatikeipq gksykvefde ltlllagnye cvatnkygsa strgkltvqs kpkfekkpen
     1621 qcvysgeecv feakvsgfpt pnvtwsrdge tieesakfti sshngthrlt vkdakkddec
     1681 fytctaknnl grvsqqaelt iklpddaeap tfirrmtdtv avigdkvrfe vrvagkptpk
     1741 vtwykdgeel fegrrveike dgdthtitlk dlvmrdeaky sckavnekge atdkaqltvk
     1801 epvapaikgl kdqeastgst vkfqatitgi papdviwkkd gkelspshki tissdvetes
     1861 ytlvihdvdd dfgqytckas nsageteada mltlkgeppv fikeledvkv tineeavfhs
     1921 rikgnptpsa twllngsilt esedfsitqh gdsytlkitk vtshdvgevv ckakntagqa
     1981 sskaglslke taptvakslp skvrvedgep lkleakvpgh pepdvqwlkd gepivpsdhv
     2041 kltqepdgtv alevdeirpd daghyavvan ndkgkaates dvetkpkgdd svkspafseg
     2101 lkdatltegk pghleaklak gsapsdvqwl kdgepvkpsd hvkmvekpdg tialdidkvt
     2161 psdaghysvm aendegkaas dadvktkpsd legkpvfeeg lkpttltege pgrleakmpa
     2221 eaaapvewtk dgapvkptdh vklveepdgt valeldevnp ddaghyavka tndkgstqse
     2281 advktqpkga epskprfekg lqpatlpegk parleakvap dtlpikvewl kdgkpvkldd
     2341 rvraeqkpdg tlalvidnvk pqdaghyavv asndagktps eadvdtipkk aakdqkpife
     2401 kglaptelte gkpgkleakl qpgdkpldiq wlkdgkpirp sdrvkpeekp dgtvclsidd
     2461 vkpedagrys vvaknpdget aseadvttkp dktaakpeka aakpkkpafe kdlepatlte
     2521 gkparleakl apgsnptnvk wlkdgrpirp ssrvkpeekp dgtlalvidd vrpedagnys
     2581 vvaendagea eseadiktqp ekyrkqrkpa fekelapttl tegkparlea klepdsnpts
     2641 vkwmkdgrpl rpsdrikmee kpdgtlalli ddvrpedagn ysvvaandag eaeseadikt
     2701 qpdkyrkqrr pafekelqpa tlpegkparl eaklepdsnp tsikwlkdgr pirpsdrirt
     2761 eerpdgtlal viddvrpeda ghysvvaand ageaasdadv ktkpearvpg ekpefvielk
     2821 nadllegqpl hlvgkvksds pfttewlkdg kkvepslrla lsqepdgtvl lsiehatpdd
     2881 agkyvcvakn pegkaessst vkvaelpkye peiveelkpa vftegepgkl eakvsgdpmp
     2941 dvkwikdgke lpedsrirst tspggdvalt idpvkpedag kydlvaande gecrtsapvt
     3001 vnhppkfvkp lepvevvegy parlettlag qptpdtewqk dgkplipdgk niketktpsg
     3061 dvaleipkat pddagvytck aknplgeket kapltvagsd tgdkaeekpt vsplddvevm
     3121 esepftleak itgkptpeve wfyggkpipr aadlettfdg rkarlsskhs lpshagryec
     3181 ratnsagkas skatvgvtpm skpyftkrlt dleaspnepl klvtkvkgyp epeitwrfng
     3241 kviepsmryt mskegevctl tvpwpeskdc gvyeclaknp vgedkcsgri tirekgepps
     3301 fvkrlenlri pegdtatfta riagtplpev kwfkdgeele ltrrhktele pngnlrltir
     3361 dckpedfgey rasiynpygs dtcmatlrve spyddgkrrp stdkypsrdi pektdvrrgr
     3421 spsprqypev ngyrappprd errrepysgp yshelpptsp lpgrdfdrpd ydpksrhpph
     3481 pryddldrpy dritrpdlep epyyagvpdp ltdkpyirrm tdrdltlgwk pslptrsrip
     3541 vtytvemapl pdgnwspyks gvkdtqldvr glepfkdyrf rvrvgnkhgl sdpspyvtah
     3601 rsrlggsppv pkefkpkdfd iphppldkpg eapvfvrhee dvmygirghp vsiefwvygn
     3661 pqpditwtfk gtkvemggry nsmqdrngqv tlfisrmted nvgaytctav nehgqatkti
     3721 ylelaeepvf tkrlepttim lrrggelqcr vigkpypkik wfkdwqpicn tsrvniiwea
     3781 pdvctlalng siaadaglys csasniagta scsamlsiee sesefdwrty rqpkiirpks
     3841 thledyynlg delgrgtqgv tyhaverstg rsfgaktmhg rgqfkdwmks eldmmnqlch
     3901 prllrlwdaf etknsmslit dfcgggplld siisqdrvse hqianyikqi leglnymhaq
     3961 niahlgltlg dalvarensd dikigdfsls trlprgkvvi qeyghpefva peiankkpas
     4021 lasdmwsvgv isyillsgis pflglndret lrnvqdgkin flhdgfakvs deardfiskl
     4081 lvfepsermd vktalehpwl kiterpdrge glsnidrlke yqrhwrnwya nascrntyrr
     4141 rpmescfthp skmiyppdev ytppmspdre isrskvksap iegpgkgpnd rmdissessy
     4201 qsgpdtyllq lrdvdfplri rqylrvgasr spslaaslre rhwggfdine rgtrnvypqv
     4261 vvrerrkfvd vmdeeiddek kglgtrtlpl rlqrevgslg yahhqmehlk heawkdktsr
     4321 eksigmapff rekmedcviq endevvfrcl avgnptpqys wfrndsilie ssrvqitqdp
     4381 dgrselrlkp gkaydvglfk caarnshgva lcharlkige kpsrpeppic kqcsshqayv
     4441 qwyspkydgn srifcfcley ketlgaewtk vsdsitqefw vipdlkpats ymfrvsaknk
     4501 ygwsetslss dpcttadega aqkiqlsreh khqeeifege yeletrvpld ygqetspvql
     4561 esgqlndlyt fsselavgrf gavvsgtskk lssglalkvv lintdpetsa hreyevlksl
     4621 rherviclkt asvhddvvvl gleklcgmdv ltflsmrhty nedmvsriik qvldgleylh
     4681 frsicyielq pdnvvltdah scniklvdfg sanfvprtgk ilpldtsacl eylapevlkg
     4741 hevchatdvw gvgvltyill sghspflgvn eedtktnily vryhfdklyk easteatrfl
     4801 mqlfkrtpek rpaiseclen kwmlpsefml rkrensvfls hklqefaanf hlkkknasta
     4861 erlskliglq lsrsslssie gsgda
//
LOCUS       XP_077502593            4857 aa            linear   INV 25-NOV-2025
DEFINITION  obscurin isoform X5 [Amblyomma americanum].
ACCESSION   XP_077502593
VERSION     XP_077502593.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646467.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..4857
                     /product="obscurin isoform X5"
                     /calculated_mol_wt=538127
     Region          228..288
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     Site            order(233,235,238,244,262..263,282,284..285)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212690"
     Region          312..489
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          496..600
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          707..796
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          724..728
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          737..741
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          762..766
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          776..781
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          789..792
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          821..911
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          838..842
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          851..855
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          877..881
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          891..896
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          904..907
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <935..1003
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          942..946
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          970..973
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          983..988
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          996..999
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1015..1105
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1032..1036
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1045..1049
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1071..1075
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1085..1090
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1098..1101
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1109..1192
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1126..1130
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1139..1143
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1164..1169
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1179..1184
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1192..1195
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1203..1294
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1220..1224
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1233..1237
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1260..1264
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1274..1279
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1287..1290
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1298..1390
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1316..1320
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1329..1333
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1356..1360
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1370..1375
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1383..1386
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1394..1483
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1411..1415
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1424..1428
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1451..1455
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1465..1470
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1478..1481
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1489..1578
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1506..1510
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1519..1523
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1544..1548
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1558..1563
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1571..1574
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1582..1671
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1599..1603
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1612..1616
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1634..1641
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1651..1656
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1664..1667
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1675..1764
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1692..1696
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1705..1709
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1730..1734
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1744..1749
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1757..1760
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1773..1862
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1790..1794
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1803..1807
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1829..1832
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1842..1847
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1855..1858
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1873..1956
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1884..1888
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1897..1901
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1924..1928
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1937..1942
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1950..1953
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1962..2049
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1979..1983
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1992..1996
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2017..2021
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2031..2036
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2044..2047
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2063..2146
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2074..2078
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2087..2091
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2113..2117
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2127..2132
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2140..2143
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2158..2248
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2175..2179
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2189..2193
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2215..2219
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2229..2234
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2242..2245
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2258..2347
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2275..2279
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2288..2292
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2314..2318
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2328..2333
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2341..2344
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2358..2445
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2375..2379
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2389..2393
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2415..2419
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2429..2434
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2442..2445
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2460..2550
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2477..2481
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2491..2495
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2517..2521
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2531..2536
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2544..2547
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2572..2657
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2587..2591
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2601..2605
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2627..2631
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2641..2646
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2654..2657
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2672..2759
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2689..2693
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2703..2707
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2729..2733
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2743..2748
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2756..2759
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2778..2864
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2791..2795
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2805..2809
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2831..2835
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2845..2850
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2858..2861
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2876..2966
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2893..2897
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2906..2910
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2932..2936
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2946..2951
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2959..2962
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2974..3064
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2991..2995
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3004..3008
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3030..3034
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3044..3049
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3057..3060
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3068..3159
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3085..3089
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3098..3102
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3125..3129
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3139..3144
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3152..3155
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3174..3260
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3188..3192
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3201..3205
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3226..3230
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3240..3245
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3253..3256
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3266..3355
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3296..3300
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3321..3325
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3335..3340
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3362..3452
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3379..3383
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3392..3396
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3419..3422
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3432..3437
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3445..3448
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3480..3571
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(3480,3546,3561)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(3562..3563,3565..3566)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          3623..3627
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          <3629..3695
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3636..3640
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3662..3666
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3676..3681
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3700..3791
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3717..3721
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3730..3734
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3756..3760
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3770..3775
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3783..3786
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3816..4072
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(3825..3828,3831,3833,3846,3848,3876,3892..3895,3938,
                     3942..3943,3945,3957..3958)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     Region          4300..4390
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4317..4321
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4330..4334
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4356..4360
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4370..4375
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4383..4386
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4394..4486
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(4394,4459,4474)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(4475..4476,4478..4479)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          4538..4795
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(4547..4550,4553,4555,4568,4570,4598,4614..4617,4660,
                     4664..4665,4667,4679..4680)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..4857
                     /gene="Obsc"
                     /coded_by="XM_077646467.1:229..14802"
                     /db_xref="GeneID:144113406"
ORIGIN      
        1 msysrssysr tskfseglsd erssrtsars sslmdsdiss smtssrrmmr ttsygdeggs
       61 sfssstvrrs srlsaleddd igsriqsssv rtsrlssrnd ddfgstaisr rpfaissgds
      121 fessrkssls ssvlgesvds sasralrrls kdddkesfas krskltskss fdvdessasv
      181 rysstkitss iessdlggas ysaskasysy rdsagsatep eagviggiye aildykplss
      241 deeglplkeg qevevidtsk prkwrvrtrs srtgisqegw vpccylekkq gavieeqvap
      301 kdresyakre avvkelvete edfsrdmqrv vnnylkemen pimpkelrdq kdvlfsnfkd
      361 icdfhnteli kgvqfnasep aklgvtflrl erdfdkhvky cedfpraqel lqsgplkeyf
      421 ddfsakindd ktlsdhlklp iqrindyqll lkelikytar lhedytdlqr aldfmqaipq
      481 rasdlqyina iegyhgnihn wgrvikhgwf diidaknreq lrylflfkgr lcitevkkir
      541 anrniylvkn vlktseseiv etgdddatlk ilqkdssgqd vpitlrakdi eqkkewarel
      601 qasrvasevv eemqlddtse rqqekssysr ttslsestav pvspdpasas mpraeifeas
      661 keatsaaths vrsgsvtsid eiqslnsfae desvyltask kdatgkpqfr kalrgltcsq
      721 ndntflecev sspekptiqw lkdniplkks srvqlseeeg kyklyindas sedsgmytii
      781 asnsegttsc saplyvkpal svakpdsrpt spggtilpha pvfkvklkdt ellegtsvrf
      841 elvvrgcpep evtvykngkk lveddrvrvm feskevfelv idhisfedmg kytcvavnse
      901 gkdessgtit vtkdkilfqg ltaddsaaeg sgpltpppks plfrwfkdgq efeaserfqv
      961 tfddiedtls lvfqhvtpdd aglytcvast ssgkiscsae ltvqgavikk apeaptiaas
     1021 lsdvevnega samlelkvkg ypkpkvtwkh egkvieaggr yrflfedees mtlvikntkk
     1081 tdagiysvva enesgtante crltvnstpt fkkelkdvsv mtddvlkldv evqgtpapdv
     1141 kwykdgqavv eddrvkitqp aedahqliik rakvedsgny scvisntmgt qtgfsavsvn
     1201 appkflqklk dyeasetenv tfrvkisgnp kpnvswkkdg kplkpdghry qtleetenvh
     1261 cliiddvhqe daaqytveia neygvetdsa altitckpkf kkplsdvecc egetnikitv
     1321 evegspapkv kwslndslic ednsfkfesv eesgkytlvi keaksdmtge mvceaanvkg
     1381 kdkcsgkftv kskpkivkgl ddvnvevgqs atfsvkvvna kdakvkwlkd dcevtidnkn
     1441 vyakeeadas ftlviksvtp edagqytfki knelgedsst grlstqkkpe fkkklqdqei
     1501 seedvaeftv vvtgepkpnv kwlhgkreik endvyeitsd gdtytlkvkk pnkelageyt
     1561 ceaqntcgke scsasltihs kpeiieglkd iegevgdaaf ftakiagspt ptvvwkkdde
     1621 eitveatike ipqgksykve fdeltlllag nyecvatnky gsastrgklt vqskpkfekk
     1681 penqcvysge ecvfeakvsg fptpnvtwsr dgetieesak ftisshngth rltvkdakkd
     1741 decfytctak nnlgrvsqqa eltiklpdda eaptfirrmt dtvavigdkv rfevrvagkp
     1801 tpkvtwykdg eelfegrrve ikedgdthti tlkdlvmrde akysckavne kgeatdkaql
     1861 tvkepvapai kglkdqeast gstvkfqati tgipapdviw kkdgkelsps hkitissdve
     1921 tesytlvihd vdddfgqytc kasnsagete adamltlkge ppvfikeled vkvtineeav
     1981 fhsrikgnpt psatwllngs iltesedfsi tqhgdsytlk itkvtshdvg evvckaknta
     2041 gqasskagls lketaptvak slpskvrved geplkleakv pghpepdvqw lkdgepivps
     2101 dhvkltqepd gtvalevdei rpddaghyav vanndkgkaa tesdvetkpk gddsvkspaf
     2161 seglkdatlt egkpghleak lakgsapsdv qwlkdgepvk psdhvkmvek pdgtialdid
     2221 kvtpsdaghy svmaendegk aasdadvktk psdlegkpvf eeglkpttlt egepgrleak
     2281 mpaeaaapve wtkdgapvkp tdhvklveep dgtvalelde vnpddaghya vkatndkgst
     2341 qseadvktqp kgaepskprf ekglqpatlp egkparleak vapdtlpikv ewlkdgkpvk
     2401 lddrvraeqk pdgtlalvid nvkpqdaghy avvasndagk tpseadvdti pkkaakdqkp
     2461 ifekglapte ltegkpgkle aklqpgdkpl diqwlkdgkp irpsdrvkpe ekpdgtvcls
     2521 iddvkpedag rysvvaknpd getaseadvt tkpdktaakp ekaaakpkkp afekdlepat
     2581 ltegkparle aklapgsnpt nvkwlkdgrp irpssrvkpe ekpdgtlalv iddvrpedag
     2641 nysvvaenda geaeseadik tqpekyrkqr kpafekelap ttltegkpar leaklepdsn
     2701 ptsvkwmkdg rplrpsdrik meekpdgtla lliddvrped agnysvvaan dageaesead
     2761 iktqpdkyrk qrrpafekel qpatlpegkp arleaklepd snptsikwlk dgrpirpsdr
     2821 irteerpdgt lalviddvrp edaghysvva andageaasd advktkpear vpgekpefvi
     2881 elknadlleg qplhlvgkvk sdspfttewl kdgkkvepsl rlalsqepdg tvllsiehat
     2941 pddagkyvcv aknpegkaes sstvkvaelp kyepeiveel kpavftegep gkleakvsgd
     3001 pmpdvkwikd gkelpedsri rsttspggdv altidpvkpe dagkydlvaa ndegecrtsa
     3061 pvtvnhppkf vkplepvevv egyparlett lagqptpdte wqkdgkplip dgkniketkt
     3121 psgdvaleip katpddagvy tckaknplge ketkapltva gsdtgdkaee kptvsplddv
     3181 evmesepftl eakitgkptp evewfyggkp ipraadlett fdgrkarlss khslpshagr
     3241 yecratnsag kasskatvgv tpmskpyftk rltdleaspn eplklvtkvk gypepeitwr
     3301 fngkviepsm rytmskegev ctltvpwpes kdcgvyecla knpvgedkcs gritirekge
     3361 ppsfvkrlen lripegdtat ftariagtpl pevkwfkdge eleltrrhkt elepngnlrl
     3421 tirdckpedf geyrasiynp ygsdtcmatl rvespyddgk rrpstdkyps rdipektgvp
     3481 dpltdkpyir rmtdrdltlg wkpslptrsr ipvtytvema plpdgnwspy ksgvkdtqld
     3541 vrglepfkdy rfrvrvgnkh glsdpspyvt ahrsrlggsp pvpkefkpkd fdiphppldk
     3601 pgeapvfvrh eedvmygirg hpvsiefwvy gnpqpditwt fkgtkvemgg rynsmqdrng
     3661 qvtlfisrmt ednvgaytct avnehgqatk tiylelaeep vftkrleptt imlrrggelq
     3721 crvigkpypk ikwfkdwqpi cntsrvniiw eapdvctlal ngsiaadagl yscsasniag
     3781 tascsamlsi eesesefdwr tyrqpkiirp ksthledyyn lgdelgrgtq gvtyhavers
     3841 tgrsfgaktm hgrgqfkdwm kseldmmnql chprllrlwd afetknsmsl itdfcgggpl
     3901 ldsiisqdrv sehqianyik qileglnymh aqniahlglt lgdalvaren sddikigdfs
     3961 lstrlprgkv viqeyghpef vapeiankkp aslasdmwsv gvisyillsg ispflglndr
     4021 etlrnvqdgk inflhdgfak vsdeardfis kllvfepser mdvktalehp wlkiterpdr
     4081 geglsnidrl keyqrhwrnw yanascrnty rrrpmescft hpskmiyppd evytppmspd
     4141 reisrskvks apiegpgkgp ndrmdisses syqsgpdtyl lqlrdvdfpl rirqylrvga
     4201 srspslaasl rerhwggfdi nergtrnvyp qvvvrerrkf vdvmdeeidd ekkglgtrtl
     4261 plrlqrevgs lgyahhqmeh lkheawkdkt sreksigmap ffrekmedcv iqendevvfr
     4321 clavgnptpq yswfrndsil iessrvqitq dpdgrselrl kpgkaydvgl fkcaarnshg
     4381 valcharlki gekpsrpepp ickqcsshqa yvqwyspkyd gnsrifcfcl eyketlgaew
     4441 tkvsdsitqe fwvipdlkpa tsymfrvsak nkygwsetsl ssdpcttade gaaqkiqlsr
     4501 ehkhqeeife geyeletrvp ldygqetspv qlesgqlndl ytfsselavg rfgavvsgts
     4561 kklssglalk vvlintdpet sahreyevlk slrhervicl ktasvhddvv vlgleklcgm
     4621 dvltflsmrh tynedmvsri ikqvldgley lhfrsicyie lqpdnvvltd ahscniklvd
     4681 fgsanfvprt gkilpldtsa cleylapevl kghevchatd vwgvgvltyi llsghspflg
     4741 vneedtktni lyvryhfdkl ykeasteatr flmqlfkrtp ekrpaisecl enkwmlpsef
     4801 mlrkrensvf lshklqefaa nfhlkkknas taerlsklig lqlsrsslss iegsgda
//
LOCUS       XP_077502594            4856 aa            linear   INV 25-NOV-2025
DEFINITION  obscurin isoform X6 [Amblyomma americanum].
ACCESSION   XP_077502594
VERSION     XP_077502594.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646468.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..4856
                     /product="obscurin isoform X6"
                     /calculated_mol_wt=539023
     Region          228..288
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     Site            order(233,235,238,244,262..263,282,284..285)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212690"
     Region          312..489
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          496..600
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          707..796
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          724..728
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          737..741
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          762..766
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          776..781
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          789..792
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          821..911
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          838..842
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          851..855
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          877..881
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          891..896
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          904..907
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          923..1013
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          940..944
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          953..957
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          979..983
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          993..998
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1006..1009
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1017..1100
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1034..1038
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1047..1051
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1072..1077
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1087..1092
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1100..1103
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1111..1202
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1128..1132
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1141..1145
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1168..1172
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1182..1187
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1195..1198
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1206..1298
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1224..1228
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1237..1241
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1264..1268
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1278..1283
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1291..1294
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1302..1391
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1319..1323
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1332..1336
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1359..1363
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1373..1378
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1386..1389
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1397..1486
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1414..1418
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1427..1431
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1452..1456
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1466..1471
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1479..1482
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1490..1579
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1507..1511
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1520..1524
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1542..1549
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1559..1564
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1572..1575
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1583..1672
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1600..1604
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1613..1617
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1638..1642
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1652..1657
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1665..1668
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1681..1770
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1698..1702
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1711..1715
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1737..1740
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1750..1755
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1763..1766
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1781..1864
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1792..1796
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1805..1809
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1832..1836
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1845..1850
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1858..1861
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1870..1957
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1887..1891
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1900..1904
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1925..1929
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1939..1944
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1952..1955
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1971..2054
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1982..1986
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1995..1999
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2021..2025
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2035..2040
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2048..2051
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2066..2156
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2083..2087
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2097..2101
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2123..2127
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2137..2142
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2150..2153
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2166..2255
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2183..2187
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2196..2200
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2222..2226
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2236..2241
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2249..2252
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2266..2353
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2283..2287
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2297..2301
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2323..2327
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2337..2342
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2350..2353
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2368..2458
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2385..2389
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2399..2403
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2425..2429
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2439..2444
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2452..2455
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2480..2565
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2495..2499
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2509..2513
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2535..2539
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2549..2554
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2562..2565
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2580..2667
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2597..2601
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2611..2615
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2637..2641
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2651..2656
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2664..2667
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2686..2772
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2699..2703
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2713..2717
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2739..2743
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2753..2758
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2766..2769
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2784..2874
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2801..2805
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2814..2818
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2840..2844
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2854..2859
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2867..2870
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2882..2972
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2899..2903
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2912..2916
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2938..2942
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2952..2957
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2965..2968
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2976..3067
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2993..2997
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3006..3010
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3033..3037
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3047..3052
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3060..3063
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3082..3168
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3096..3100
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3109..3113
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3134..3138
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3148..3153
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3161..3164
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3174..3263
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3204..3208
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3229..3233
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3243..3248
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3270..3360
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3287..3291
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3300..3304
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3327..3330
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3340..3345
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3353..3356
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3479..3570
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(3479,3545,3560)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(3561..3562,3564..3565)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          3622..3626
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          <3628..3694
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3635..3639
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3661..3665
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3675..3680
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3699..3790
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3716..3720
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3729..3733
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3755..3759
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3769..3774
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3782..3785
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3815..4071
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(3824..3827,3830,3832,3845,3847,3875,3891..3894,3937,
                     3941..3942,3944,3956..3957)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     Region          4299..4389
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4316..4320
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4329..4333
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4355..4359
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4369..4374
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4382..4385
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4393..4485
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(4393,4458,4473)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(4474..4475,4477..4478)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          4537..4794
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(4546..4549,4552,4554,4567,4569,4597,4613..4616,4659,
                     4663..4664,4666,4678..4679)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..4856
                     /gene="Obsc"
                     /coded_by="XM_077646468.1:229..14799"
                     /db_xref="GeneID:144113406"
ORIGIN      
        1 msysrssysr tskfseglsd erssrtsars sslmdsdiss smtssrrmmr ttsygdeggs
       61 sfssstvrrs srlsaleddd igsriqsssv rtsrlssrnd ddfgstaisr rpfaissgds
      121 fessrkssls ssvlgesvds sasralrrls kdddkesfas krskltskss fdvdessasv
      181 rysstkitss iessdlggas ysaskasysy rdsagsatep eagviggiye aildykplss
      241 deeglplkeg qevevidtsk prkwrvrtrs srtgisqegw vpccylekkq gavieeqvap
      301 kdresyakre avvkelvete edfsrdmqrv vnnylkemen pimpkelrdq kdvlfsnfkd
      361 icdfhnteli kgvqfnasep aklgvtflrl erdfdkhvky cedfpraqel lqsgplkeyf
      421 ddfsakindd ktlsdhlklp iqrindyqll lkelikytar lhedytdlqr aldfmqaipq
      481 rasdlqyina iegyhgnihn wgrvikhgwf diidaknreq lrylflfkgr lcitevkkir
      541 anrniylvkn vlktseseiv etgdddatlk ilqkdssgqd vpitlrakdi eqkkewarel
      601 qasrvasevv eemqlddtse rqqekssysr ttslsestav pvspdpasas mpraeifeas
      661 keatsaaths vrsgsvtsid eiqslnsfae desvyltask kdatgkpqfr kalrgltcsq
      721 ndntflecev sspekptiqw lkdniplkks srvqlseeeg kyklyindas sedsgmytii
      781 asnsegttsc saplyvkpal svakpdsrpt spggtilpha pvfkvklkdt ellegtsvrf
      841 elvvrgcpep evtvykngkk lveddrvrvm feskevfelv idhisfedmg kytcvavnse
      901 gkdessgtit vtsavikkap eaptiaasls dvevnegasa mlelkvkgyp kpkvtwkheg
      961 kvieaggryr flfedeesmt lvikntkktd agiysvvaen esgtantecr ltvnstptfk
     1021 kelkdvsvmt ddvlkldvev qgtpapdvkw ykdgqavved drvkitqpae dahqliikra
     1081 kvedsgnysc visntmgtqt gfsavsvnap pkflqklkdy easetenvtf rvkisgnpkp
     1141 nvswkkdgkp lkpdghryqt leetenvhcl iiddvhqeda aqytveiane ygvetdsaal
     1201 titckpkfkk plsdvecceg etnikitvev egspapkvkw slndsliced nsfkfesvee
     1261 sgkytlvike aksdmtgemv ceaanvkgkd kcsgkftvks kpkivkgldd vnvevgqsat
     1321 fsvkvvnakd akvkwlkddc evtidnknvy akeeadasft lviksvtped agqytfkikn
     1381 elgedsstgr lstqkkpefk kklqdqeise edvaeftvvv tgepkpnvkw lhgkreiken
     1441 dvyeitsdgd tytlkvkkpn kelageytce aqntcgkesc sasltihskp eiieglkdie
     1501 gevgdaafft akiagsptpt vvwkkddeei tveatikeip qgksykvefd eltlllagny
     1561 ecvatnkygs astrgkltvq skpkfekkpe nqcvysgeec vfeakvsgfp tpnvtwsrdg
     1621 etieesakft isshngthrl tvkdakkdde cfytctaknn lgrvsqqael tiklpddaea
     1681 ptfirrmtdt vavigdkvrf evrvagkptp kvtwykdgee lfegrrveik edgdthtitl
     1741 kdlvmrdeak ysckavnekg eatdkaqltv kepvapaikg lkdqeastgs tvkfqatitg
     1801 ipapdviwkk dgkelspshk itissdvete sytlvihdvd ddfgqytcka snsagetead
     1861 amltlkgepp vfikeledvk vtineeavfh srikgnptps atwllngsil tesedfsitq
     1921 hgdsytlkit kvtshdvgev vckakntagq asskaglslk etaptvaksl pskvrvedge
     1981 plkleakvpg hpepdvqwlk dgepivpsdh vkltqepdgt valevdeirp ddaghyavva
     2041 nndkgkaate sdvetkpkgd dsvkspafse glkdatlteg kpghleakla kgsapsdvqw
     2101 lkdgepvkps dhvkmvekpd gtialdidkv tpsdaghysv maendegkaa sdadvktkps
     2161 dlegkpvfee glkpttlteg epgrleakmp aeaaapvewt kdgapvkptd hvklveepdg
     2221 tvaleldevn pddaghyavk atndkgstqs eadvktqpkg aepskprfek glqpatlpeg
     2281 kparleakva pdtlpikvew lkdgkpvkld drvraeqkpd gtlalvidnv kpqdaghyav
     2341 vasndagktp seadvdtipk kaakdqkpif ekglaptelt egkpgkleak lqpgdkpldi
     2401 qwlkdgkpir psdrvkpeek pdgtvclsid dvkpedagry svvaknpdge taseadvttk
     2461 pdktaakpek aaakpkkpaf ekdlepatlt egkparleak lapgsnptnv kwlkdgrpir
     2521 pssrvkpeek pdgtlalvid dvrpedagny svvaendage aeseadiktq pekyrkqrkp
     2581 afekelaptt ltegkparle aklepdsnpt svkwmkdgrp lrpsdrikme ekpdgtlall
     2641 iddvrpedag nysvvaanda geaeseadik tqpdkyrkqr rpafekelqp atlpegkpar
     2701 leaklepdsn ptsikwlkdg rpirpsdrir teerpdgtla lviddvrped aghysvvaan
     2761 dageaasdad vktkpearvp gekpefviel knadllegqp lhlvgkvksd spfttewlkd
     2821 gkkvepslrl alsqepdgtv llsiehatpd dagkyvcvak npegkaesss tvkvaelpky
     2881 epeiveelkp avftegepgk leakvsgdpm pdvkwikdgk elpedsrirs ttspggdval
     2941 tidpvkpeda gkydlvaand egecrtsapv tvnhppkfvk plepvevveg yparlettla
     3001 gqptpdtewq kdgkplipdg kniketktps gdvaleipka tpddagvytc kaknplgeke
     3061 tkapltvags dtgdkaeekp tvsplddvev mesepftlea kitgkptpev ewfyggkpip
     3121 raadlettfd grkarlsskh slpshagrye cratnsagka sskatvgvtp mskpyftkrl
     3181 tdleaspnep lklvtkvkgy pepeitwrfn gkviepsmry tmskegevct ltvpwpeskd
     3241 cgvyeclakn pvgedkcsgr itirekgepp sfvkrlenlr ipegdtatft ariagtplpe
     3301 vkwfkdgeel eltrrhktel epngnlrlti rdckpedfge yrasiynpyg sdtcmatlrv
     3361 espyddgkrr pstdkypsrd ipektdvrrg rspsprqype vngyrapppr derrrepysg
     3421 pyshelppts plpgrdfdrp dydpksrhpp hpryddldrp ydritrpdle pepyyagvpd
     3481 pltdkpyirr mtdrdltlgw kpslptrsri pvtytvemap lpdgnwspyk sgvkdtqldv
     3541 rglepfkdyr frvrvgnkhg lsdpspyvta hrsrlggspp vpkefkpkdf diphppldkp
     3601 geapvfvrhe edvmygirgh pvsiefwvyg npqpditwtf kgtkvemggr ynsmqdrngq
     3661 vtlfisrmte dnvgaytcta vnehgqatkt iylelaeepv ftkrleptti mlrrggelqc
     3721 rvigkpypki kwfkdwqpic ntsrvniiwe apdvctlaln gsiaadagly scsasniagt
     3781 ascsamlsie esesefdwrt yrqpkiirpk sthledyynl gdelgrgtqg vtyhaverst
     3841 grsfgaktmh grgqfkdwmk seldmmnqlc hprllrlwda fetknsmsli tdfcgggpll
     3901 dsiisqdrvs ehqianyikq ileglnymha qniahlgltl gdalvarens ddikigdfsl
     3961 strlprgkvv iqeyghpefv apeiankkpa slasdmwsvg visyillsgi spflglndre
     4021 tlrnvqdgki nflhdgfakv sdeardfisk llvfepserm dvktalehpw lkiterpdrg
     4081 eglsnidrlk eyqrhwrnwy anascrntyr rrpmescfth pskmiyppde vytppmspdr
     4141 eisrskvksa piegpgkgpn drmdissess yqsgpdtyll qlrdvdfplr irqylrvgas
     4201 rspslaaslr erhwggfdin ergtrnvypq vvvrerrkfv dvmdeeidde kkglgtrtlp
     4261 lrlqrevgsl gyahhqmehl kheawkdkts reksigmapf frekmedcvi qendevvfrc
     4321 lavgnptpqy swfrndsili essrvqitqd pdgrselrlk pgkaydvglf kcaarnshgv
     4381 alcharlkig ekpsrpeppi ckqcsshqay vqwyspkydg nsrifcfcle yketlgaewt
     4441 kvsdsitqef wvipdlkpat symfrvsakn kygwsetsls sdpcttadeg aaqkiqlsre
     4501 hkhqeeifeg eyeletrvpl dygqetspvq lesgqlndly tfsselavgr fgavvsgtsk
     4561 klssglalkv vlintdpets ahreyevlks lrherviclk tasvhddvvv lgleklcgmd
     4621 vltflsmrht ynedmvsrii kqvldgleyl hfrsicyiel qpdnvvltda hscniklvdf
     4681 gsanfvprtg kilpldtsac leylapevlk ghevchatdv wgvgvltyil lsghspflgv
     4741 needtktnil yvryhfdkly keasteatrf lmqlfkrtpe krpaisecle nkwmlpsefm
     4801 lrkrensvfl shklqefaan fhlkkknast aerlskligl qlsrsslssi egsgda
//
LOCUS       XP_077502595            4793 aa            linear   INV 25-NOV-2025
DEFINITION  obscurin isoform X7 [Amblyomma americanum].
ACCESSION   XP_077502595
VERSION     XP_077502595.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646469.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..4793
                     /product="obscurin isoform X7"
                     /calculated_mol_wt=531516
     Region          228..288
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     Site            order(233,235,238,244,262..263,282,284..285)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212690"
     Region          312..489
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          644..733
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          661..665
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          674..678
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          699..703
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          713..718
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          726..729
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          758..848
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          775..779
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          788..792
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          814..818
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          828..833
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          841..844
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          860..950
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          877..881
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          890..894
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          916..920
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          930..935
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          943..946
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          954..1037
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          971..975
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          984..988
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1009..1014
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1024..1029
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1037..1040
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1048..1139
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1065..1069
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1078..1082
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1105..1109
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1119..1124
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1132..1135
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1143..1235
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1161..1165
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1174..1178
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1201..1205
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1215..1220
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1228..1231
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1239..1328
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1256..1260
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1269..1273
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1296..1300
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1310..1315
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1323..1326
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1334..1423
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1351..1355
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1364..1368
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1389..1393
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1403..1408
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1416..1419
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1427..1516
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1444..1448
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1457..1461
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1479..1486
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1496..1501
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1509..1512
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1520..1609
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1537..1541
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1550..1554
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1575..1579
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1589..1594
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1602..1605
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1618..1707
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1635..1639
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1648..1652
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1674..1677
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1687..1692
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1700..1703
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1718..1801
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1729..1733
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1742..1746
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1769..1773
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1782..1787
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1795..1798
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1807..1894
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1824..1828
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1837..1841
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1862..1866
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1876..1881
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1889..1892
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1908..1991
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1919..1923
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1932..1936
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1958..1962
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1972..1977
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1985..1988
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2003..2093
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2020..2024
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2034..2038
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2060..2064
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2074..2079
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2087..2090
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2103..2192
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2120..2124
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2133..2137
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2159..2163
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2173..2178
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2186..2189
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2203..2290
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2220..2224
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2234..2238
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2260..2264
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2274..2279
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2287..2290
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2305..2395
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2322..2326
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2336..2340
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2362..2366
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2376..2381
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2389..2392
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2417..2502
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2432..2436
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2446..2450
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2472..2476
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2486..2491
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2499..2502
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2517..2604
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2534..2538
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2548..2552
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2574..2578
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2588..2593
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2601..2604
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2623..2709
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2636..2640
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2650..2654
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2676..2680
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2690..2695
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2703..2706
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2721..2811
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2738..2742
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2751..2755
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2777..2781
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2791..2796
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2804..2807
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2819..2909
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2836..2840
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2849..2853
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2875..2879
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2889..2894
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2902..2905
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2913..3004
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2930..2934
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2943..2947
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2970..2974
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2984..2989
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2997..3000
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3019..3105
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3033..3037
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3046..3050
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3071..3075
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3085..3090
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3098..3101
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3111..3200
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3141..3145
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3166..3170
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3180..3185
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3207..3297
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3224..3228
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3237..3241
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3264..3267
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3277..3282
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3290..3293
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3416..3507
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(3416,3482,3497)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(3498..3499,3501..3502)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          3559..3563
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          <3565..3631
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3572..3576
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3598..3602
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3612..3617
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3636..3727
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3653..3657
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3666..3670
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3692..3696
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3706..3711
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3719..3722
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3752..4008
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(3761..3764,3767,3769,3782,3784,3812,3828..3831,3874,
                     3878..3879,3881,3893..3894)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     Region          4236..4326
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4253..4257
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4266..4270
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4292..4296
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4306..4311
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4319..4322
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4330..4422
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(4330,4395,4410)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(4411..4412,4414..4415)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          4474..4731
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(4483..4486,4489,4491,4504,4506,4534,4550..4553,4596,
                     4600..4601,4603,4615..4616)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..4793
                     /gene="Obsc"
                     /coded_by="XM_077646469.1:229..14610"
                     /db_xref="GeneID:144113406"
ORIGIN      
        1 msysrssysr tskfseglsd erssrtsars sslmdsdiss smtssrrmmr ttsygdeggs
       61 sfssstvrrs srlsaleddd igsriqsssv rtsrlssrnd ddfgstaisr rpfaissgds
      121 fessrkssls ssvlgesvds sasralrrls kdddkesfas krskltskss fdvdessasv
      181 rysstkitss iessdlggas ysaskasysy rdsagsatep eagviggiye aildykplss
      241 deeglplkeg qevevidtsk prkwrvrtrs srtgisqegw vpccylekkq gavieeqvap
      301 kdresyakre avvkelvete edfsrdmqrv vnnylkemen pimpkelrdq kdvlfsnfkd
      361 icdfhnteli kgvqfnasep aklgvtflrl erdfdkhvky cedfpraqel lqsgplkeyf
      421 ddfsakindd ktlsdhlklp iqrindyqll lkelikytar lhedytdlqr aldfmqaipq
      481 rasdlqyina iegyhgnihn wgrvikhtse seivetgddd atlkilqkds sgqdvpitlr
      541 akdieqkkew arelqasrva sevveemqld dtserqqeks sysrttslse stavpvspdp
      601 asasmpraei feaskeatsa athsvrsgsv tsidaskkda tgkpqfrkal rgltcsqndn
      661 tflecevssp ekptiqwlkd niplkkssrv qlseeegkyk lyindassed sgmytiiasn
      721 segttscsap lyvkpalsva kpdsrptspg gtilphapvf kvklkdtell egtsvrfelv
      781 vrgcpepevt vykngkklve ddrvrvmfes kevfelvidh isfedmgkyt cvavnsegkd
      841 essgtitvts avikkapeap tiaaslsdve vnegasamle lkvkgypkpk vtwkhegkvi
      901 eaggryrflf edeesmtlvi kntkktdagi ysvvaenesg tantecrltv nstptfkkel
      961 kdvsvmtddv lkldvevqgt papdvkwykd gqavveddrv kitqpaedah qliikrakve
     1021 dsgnyscvis ntmgtqtgfs avsvnappkf lqklkdyeas etenvtfrvk isgnpkpnvs
     1081 wkkdgkplkp dghryqtlee tenvhcliid dvhqedaaqy tveianeygv etdsaaltit
     1141 ckpkfkkpls dveccegetn ikitvevegs papkvkwsln dslicednsf kfesveesgk
     1201 ytlvikeaks dmtgemvcea anvkgkdkcs gkftvkskpk ivkglddvnv evgqsatfsv
     1261 kvvnakdakv kwlkddcevt idnknvyake eadasftlvi ksvtpedagq ytfkiknelg
     1321 edsstgrlst qkkpefkkkl qdqeiseedv aeftvvvtge pkpnvkwlhg kreikendvy
     1381 eitsdgdtyt lkvkkpnkel ageytceaqn tcgkescsas ltihskpeii eglkdiegev
     1441 gdaafftaki agsptptvvw kkddeeitve atikeipqgk sykvefdelt lllagnyecv
     1501 atnkygsast rgkltvqskp kfekkpenqc vysgeecvfe akvsgfptpn vtwsrdgeti
     1561 eesakftiss hngthrltvk dakkddecfy tctaknnlgr vsqqaeltik lpddaeaptf
     1621 irrmtdtvav igdkvrfevr vagkptpkvt wykdgeelfe grrveikedg dthtitlkdl
     1681 vmrdeakysc kavnekgeat dkaqltvkep vapaikglkd qeastgstvk fqatitgipa
     1741 pdviwkkdgk elspshkiti ssdvetesyt lvihdvdddf gqytckasns ageteadaml
     1801 tlkgeppvfi keledvkvti neeavfhsri kgnptpsatw llngsiltes edfsitqhgd
     1861 sytlkitkvt shdvgevvck akntagqass kaglslketa ptvakslpsk vrvedgeplk
     1921 leakvpghpe pdvqwlkdge pivpsdhvkl tqepdgtval evdeirpdda ghyavvannd
     1981 kgkaatesdv etkpkgddsv kspafseglk datltegkpg hleaklakgs apsdvqwlkd
     2041 gepvkpsdhv kmvekpdgti aldidkvtps daghysvmae ndegkaasda dvktkpsdle
     2101 gkpvfeeglk pttltegepg rleakmpaea aapvewtkdg apvkptdhvk lveepdgtva
     2161 leldevnpdd aghyavkatn dkgstqsead vktqpkgaep skprfekglq patlpegkpa
     2221 rleakvapdt lpikvewlkd gkpvklddrv raeqkpdgtl alvidnvkpq daghyavvas
     2281 ndagktpsea dvdtipkkaa kdqkpifekg lapteltegk pgkleaklqp gdkpldiqwl
     2341 kdgkpirpsd rvkpeekpdg tvclsiddvk pedagrysvv aknpdgetas eadvttkpdk
     2401 taakpekaaa kpkkpafekd lepatltegk parleaklap gsnptnvkwl kdgrpirpss
     2461 rvkpeekpdg tlalviddvr pedagnysvv aendageaes eadiktqpek yrkqrkpafe
     2521 kelapttlte gkparleakl epdsnptsvk wmkdgrplrp sdrikmeekp dgtlallidd
     2581 vrpedagnys vvaandagea eseadiktqp dkyrkqrrpa fekelqpatl pegkparlea
     2641 klepdsnpts ikwlkdgrpi rpsdrirtee rpdgtlalvi ddvrpedagh ysvvaandag
     2701 eaasdadvkt kpearvpgek pefvielkna dllegqplhl vgkvksdspf ttewlkdgkk
     2761 vepslrlals qepdgtvlls iehatpddag kyvcvaknpe gkaessstvk vaelpkyepe
     2821 iveelkpavf tegepgklea kvsgdpmpdv kwikdgkelp edsrirstts pggdvaltid
     2881 pvkpedagky dlvaandege crtsapvtvn hppkfvkple pvevvegypa rlettlagqp
     2941 tpdtewqkdg kplipdgkni ketktpsgdv aleipkatpd dagvytckak nplgeketka
     3001 pltvagsdtg dkaeekptvs plddvevmes epftleakit gkptpevewf yggkpipraa
     3061 dlettfdgrk arlsskhslp shagryecra tnsagkassk atvgvtpmsk pyftkrltdl
     3121 easpneplkl vtkvkgypep eitwrfngkv iepsmrytms kegevctltv pwpeskdcgv
     3181 yeclaknpvg edkcsgriti rekgeppsfv krlenlripe gdtatftari agtplpevkw
     3241 fkdgeelelt rrhktelepn gnlrltirdc kpedfgeyra siynpygsdt cmatlrvesp
     3301 yddgkrrpst dkypsrdipe ktdvrrgrsp sprqypevng yrappprder rrepysgpys
     3361 helpptsplp grdfdrpdyd pksrhpphpr yddldrpydr itrpdlepep yyagvpdplt
     3421 dkpyirrmtd rdltlgwkps lptrsripvt ytvemaplpd gnwspyksgv kdtqldvrgl
     3481 epfkdyrfrv rvgnkhglsd pspyvtahrs rlggsppvpk efkpkdfdip hppldkpgea
     3541 pvfvrheedv mygirghpvs iefwvygnpq pditwtfkgt kvemggryns mqdrngqvtl
     3601 fisrmtednv gaytctavne hgqatktiyl elaeepvftk rlepttimlr rggelqcrvi
     3661 gkpypkikwf kdwqpicnts rvniiweapd vctlalngsi aadaglyscs asniagtasc
     3721 samlsieese sefdwrtyrq pkiirpksth ledyynlgde lgrgtqgvty haverstgrs
     3781 fgaktmhgrg qfkdwmksel dmmnqlchpr llrlwdafet knsmslitdf cgggplldsi
     3841 isqdrvsehq ianyikqile glnymhaqni ahlgltlgda lvarensddi kigdfslstr
     3901 lprgkvviqe yghpefvape iankkpasla sdmwsvgvis yillsgispf lglndretlr
     3961 nvqdgkinfl hdgfakvsde ardfiskllv fepsermdvk talehpwlki terpdrgegl
     4021 snidrlkeyq rhwrnwyana scrntyrrrp mescfthpsk miyppdevyt ppmspdreis
     4081 rskvksapie gpgkgpndrm dissessyqs gpdtyllqlr dvdfplrirq ylrvgasrsp
     4141 slaaslrerh wggfdinerg trnvypqvvv rerrkfvdvm deeiddekkg lgtrtlplrl
     4201 qrevgslgya hhqmehlkhe awkdktsrek sigmapffre kmedcviqen devvfrclav
     4261 gnptpqyswf rndsiliess rvqitqdpdg rselrlkpgk aydvglfkca arnshgvalc
     4321 harlkigekp srpeppickq csshqayvqw yspkydgnsr ifcfcleyke tlgaewtkvs
     4381 dsitqefwvi pdlkpatsym frvsaknkyg wsetslssdp cttadegaaq kiqlsrehkh
     4441 qeeifegeye letrvpldyg qetspvqles gqlndlytfs selavgrfga vvsgtskkls
     4501 sglalkvvli ntdpetsahr eyevlkslrh erviclktas vhddvvvlgl eklcgmdvlt
     4561 flsmrhtyne dmvsriikqv ldgleylhfr sicyielqpd nvvltdahsc niklvdfgsa
     4621 nfvprtgkil pldtsacley lapevlkghe vchatdvwgv gvltyillsg hspflgvnee
     4681 dtktnilyvr yhfdklykea steatrflmq lfkrtpekrp aiseclenkw mlpsefmlrk
     4741 rensvflshk lqefaanfhl kkknastaer lskliglqls rsslssiegs gda
//
LOCUS       XP_077502596             514 aa            linear   INV 25-NOV-2025
DEFINITION  PUB and ZnF_RBZ domain-containing protein tamozhennic [Amblyomma
            americanum].
ACCESSION   XP_077502596
VERSION     XP_077502596.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646470.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..514
                     /product="PUB and ZnF_RBZ domain-containing protein
                     tamozhennic"
                     /calculated_mol_wt=56150
     Region          79..166
                     /region_name="PUB"
                     /note="PNGase/UBA or UBX (PUB) domain of p97 adaptor
                     proteins; cl15262"
                     /db_xref="CDD:472791"
     Site            order(83,86..87,90,98,103..104,107,109,113)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:198416"
     Region          447..467
                     /region_name="ZnF_RBZ"
                     /note="Zinc finger domain; smart00547"
                     /db_xref="CDD:197784"
     Region          447..466
                     /region_name="RanBP2-type Zn finger"
                     /note="RanBP2-type Zn finger [structural motif]"
                     /db_xref="CDD:275376"
     Region          485..503
                     /region_name="RanBP2-type Zn finger"
                     /note="RanBP2-type Zn finger [structural motif]"
                     /db_xref="CDD:275376"
     CDS             1..514
                     /gene="tamo"
                     /coded_by="XM_077646470.1:362..1906"
                     /db_xref="GeneID:144113407"
ORIGIN      
        1 mwrsapdmvr svlskyqrec llqeegsrqa ekrqsleeaa wrlvcklpha nkfvckpvea
       61 vlrraledga sfeplqaata faaleeyatn lllqpwrtel feiklysgyy khtverhlkn
      121 aetvlqlmgy ekrensvmhl ghavdtatlg dvaldcliaa aecrvlaaim dgikdrglsl
      181 swkevfrfrr dfscdpedav ralvqkhkyk sskqpaptys anepylswrd lpgsdyqmat
      241 ngtwpsphwq sqlgatsvda seakrhpats yrassamhgt rhteelvmgs tdvlgrtvgd
      301 gdpyadaygi sasswggyct asgydlpgat srasdrsflr cdlsgdrcys vpadsmpgfl
      361 phhsgdcssg rdaishlsqe vrslslgskl sglemngmph esslaianaa lpshsdsagd
      421 cldaaavvea sqryleaees ssdkqiwscg actylnaper dvcemcsksr hlgpemtpll
      481 sggkqcplct lvndrqalsc sacsqslkds ptyi
//
LOCUS       XP_077502597             514 aa            linear   INV 25-NOV-2025
DEFINITION  PUB and ZnF_RBZ domain-containing protein tamozhennic [Amblyomma
            americanum].
ACCESSION   XP_077502597
VERSION     XP_077502597.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646471.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..514
                     /product="PUB and ZnF_RBZ domain-containing protein
                     tamozhennic"
                     /calculated_mol_wt=56150
     Region          79..166
                     /region_name="PUB"
                     /note="PNGase/UBA or UBX (PUB) domain of p97 adaptor
                     proteins; cl15262"
                     /db_xref="CDD:472791"
     Site            order(83,86..87,90,98,103..104,107,109,113)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:198416"
     Region          447..467
                     /region_name="ZnF_RBZ"
                     /note="Zinc finger domain; smart00547"
                     /db_xref="CDD:197784"
     Region          447..466
                     /region_name="RanBP2-type Zn finger"
                     /note="RanBP2-type Zn finger [structural motif]"
                     /db_xref="CDD:275376"
     Region          485..503
                     /region_name="RanBP2-type Zn finger"
                     /note="RanBP2-type Zn finger [structural motif]"
                     /db_xref="CDD:275376"
     CDS             1..514
                     /gene="tamo"
                     /coded_by="XM_077646471.1:340..1884"
                     /db_xref="GeneID:144113407"
ORIGIN      
        1 mwrsapdmvr svlskyqrec llqeegsrqa ekrqsleeaa wrlvcklpha nkfvckpvea
       61 vlrraledga sfeplqaata faaleeyatn lllqpwrtel feiklysgyy khtverhlkn
      121 aetvlqlmgy ekrensvmhl ghavdtatlg dvaldcliaa aecrvlaaim dgikdrglsl
      181 swkevfrfrr dfscdpedav ralvqkhkyk sskqpaptys anepylswrd lpgsdyqmat
      241 ngtwpsphwq sqlgatsvda seakrhpats yrassamhgt rhteelvmgs tdvlgrtvgd
      301 gdpyadaygi sasswggyct asgydlpgat srasdrsflr cdlsgdrcys vpadsmpgfl
      361 phhsgdcssg rdaishlsqe vrslslgskl sglemngmph esslaianaa lpshsdsagd
      421 cldaaavvea sqryleaees ssdkqiwscg actylnaper dvcemcsksr hlgpemtpll
      481 sggkqcplct lvndrqalsc sacsqslkds ptyi
//
LOCUS       XP_077502598             118 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113409 [Amblyomma americanum].
ACCESSION   XP_077502598
VERSION     XP_077502598.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646472.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..118
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..118
                     /product="uncharacterized protein LOC144113409"
                     /calculated_mol_wt=13861
     CDS             1..118
                     /gene="LOC144113409"
                     /coded_by="XM_077646472.1:2284..2640"
                     /db_xref="GeneID:144113409"
ORIGIN      
        1 mrrqkeawkg raqelqesel ekekeywksr aqelqddnif lkaqvaslqr cleskmfqie
       61 qgkarysqgm taetlkhwgv qksvsteeae ktltrtltek iqdllksklr kqikflkt
//
LOCUS       XP_077502599             208 aa            linear   INV 25-NOV-2025
DEFINITION  retinitis pigmentosa 9 protein-like [Amblyomma americanum].
ACCESSION   XP_077502599
VERSION     XP_077502599.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646473.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..208
                     /product="retinitis pigmentosa 9 protein-like"
                     /calculated_mol_wt=23993
     CDS             1..208
                     /gene="LOC144113410"
                     /coded_by="XM_077646473.1:137..763"
                     /db_xref="GeneID:144113410"
ORIGIN      
        1 mlsstkcdrr gsqetklrst lsdkernldn ekiqalkhid tfysqappgl iqeerenped
       61 cipdlpenrn areflskapt kglwmplgke vkvmkcwrck ayghrtgdre cpmflsgnsa
      121 seefrfvhed pmhdyvkenk atekqerirq lqalleessd sdssssssss sssstpeesr
      181 krrhkrshsk heakkkhhrk skkkhkkk
//
LOCUS       XP_077502600             763 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113411 [Amblyomma americanum].
ACCESSION   XP_077502600
VERSION     XP_077502600.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646474.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..763
                     /product="uncharacterized protein LOC144113411"
                     /calculated_mol_wt=86023
     CDS             1..763
                     /gene="LOC144113411"
                     /coded_by="XM_077646474.1:128..2419"
                     /db_xref="GeneID:144113411"
ORIGIN      
        1 mskkrctkln tdvlrkkdsn gdlislwcrp tskdsdafct lccttvhcgq hgtaavyrha
       61 ssqkhiaqak rnrnaegvlk ksskvqstld qhsatatslq ekvtraetlf alsvvannip
      121 ytygdaatat yrhmfpdset akgfqcgrkk lsyiisdglg pyfkakviqe ldmpdtfyti
      181 midespipea kvqqldvlvr yyststenvv vehlqsfhlg hatadqlfsc iedalsdvrk
      241 knmvcfysdg pnvmkslkrr lkkevspdmv digecglhkv hnafaagldm fcaeveslat
      301 dvhyyfkfat rhadmkells dvglpqlefl rhvnsrwltl lpsverilks fdalkaffsk
      361 sgqprcssmr hgrlssafcd ktlraklffl qnaaqifdrf ktlfqskdpl lhvfydemlv
      421 lvkqvlgrfl rqesfagatg sqlkeldves senwkakpei gldteqsmkl wnptekkafy
      481 ikarafyiac akylitrlpl dnkllfhlrf lnpdtkgnsf tsslryvana lpqvippcdv
      541 ssltdewnsl mcetsdwels pnvvthwssv falqtpagqa kypritklvk aalslphgna
      601 dcergfsenk qalhhrstls itsisslrqt kafmkrysgd atkvsltrdi lrnveksykv
      661 yrerieeekt atsqkrkhee eeptevcerk klmdeksslq qrlsslkall asaqeliskg
      721 vadrdmdkve sgnillcdvn sklpsvieri ktvdsalqsm kan
//
LOCUS       XP_077502601             225 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113413 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502601
VERSION     XP_077502601.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646475.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..225
                     /product="uncharacterized protein LOC144113413 isoform X1"
                     /calculated_mol_wt=24293
     CDS             1..225
                     /gene="LOC144113413"
                     /coded_by="XM_077646475.1:16..693"
                     /db_xref="GeneID:144113413"
ORIGIN      
        1 mgffsplspv rywflrssaa hifnaccrrg lralpapgek strrpaaaws qvvssllssv
       61 fslglvaciv shykmgarsw serrsrpaph dpqpyqhvll ppvqsalyss asaaerqraa
      121 laaardaqvr aasvrtdlal elpptvalpe ehplagsvrl rdadqeqely gacvrappnr
      181 tvrdgsppcg gggrlrrvvv rsnsvtpcnr tarwhspdcq rpggv
//
LOCUS       XP_077502602             224 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113413 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502602
VERSION     XP_077502602.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646476.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..224
                     /product="uncharacterized protein LOC144113413 isoform X2"
                     /calculated_mol_wt=24222
     CDS             1..224
                     /gene="LOC144113413"
                     /coded_by="XM_077646476.1:16..690"
                     /db_xref="GeneID:144113413"
ORIGIN      
        1 mgffsplspv rywflrssaa hifnaccrrg lralpapgek strrpaawsq vvssllssvf
       61 slglvacivs hykmgarsws errsrpaphd pqpyqhvllp pvqsalyssa saaerqraal
      121 aaardaqvra asvrtdlale lpptvalpee hplagsvrlr dadqeqelyg acvrappnrt
      181 vrdgsppcgg ggrlrrvvvr snsvtpcnrt arwhspdcqr pggv
//
LOCUS       XP_077502603             221 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113413 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502603
VERSION     XP_077502603.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646477.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..221
                     /product="uncharacterized protein LOC144113413 isoform X3"
                     /calculated_mol_wt=23690
     CDS             1..221
                     /gene="LOC144113413"
                     /coded_by="XM_077646477.1:161..826"
                     /db_xref="GeneID:144113413"
ORIGIN      
        1 mpttagaagp salrpicwra kyrpevcclk hakcrtstmt lvaawsqvvs sllssvfslg
       61 lvacivshyk mgarswserr srpaphdpqp yqhvllppvq salyssasaa erqraalaaa
      121 rdaqvraasv rtdlalelpp tvalpeehpl agsvrlrdad qeqelygacv rappnrtvrd
      181 gsppcggggr lrrvvvrsns vtpcnrtarw hspdcqrpgg v
//
LOCUS       XP_077502604             204 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113413 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077502604
VERSION     XP_077502604.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646478.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..204
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..204
                     /product="uncharacterized protein LOC144113413 isoform X4"
                     /calculated_mol_wt=21922
     CDS             1..204
                     /gene="LOC144113413"
                     /coded_by="XM_077646478.1:41..655"
                     /db_xref="GeneID:144113413"
ORIGIN      
        1 mlpprsarap ssrrevdata cryafaawsq vvssllssvf slglvacivs hykmgarsws
       61 errsrpaphd pqpyqhvllp pvqsalyssa saaerqraal aaardaqvra asvrtdlale
      121 lpptvalpee hplagsvrlr dadqeqelyg acvrappnrt vrdgsppcgg ggrlrrvvvr
      181 snsvtpcnrt arwhspdcqr pggv
//
LOCUS       XP_077502606             183 aa            linear   INV 25-NOV-2025
DEFINITION  protein TMEPAI-like isoform X5 [Amblyomma americanum].
ACCESSION   XP_077502606
VERSION     XP_077502606.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646480.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="protein TMEPAI-like isoform X5"
                     /calculated_mol_wt=19549
     CDS             1..183
                     /gene="LOC144113413"
                     /coded_by="XM_077646480.1:255..806"
                     /db_xref="GeneID:144113413"
ORIGIN      
        1 mlassawsqv vssllssvfs lglvacivsh ykmgarswse rrsrpaphdp qpyqhvllpp
       61 vqsalyssas aaerqraala aardaqvraa svrtdlalel pptvalpeeh plagsvrlrd
      121 adqeqelyga cvrappnrtv rdgsppcggg grlrrvvvrs nsvtpcnrta rwhspdcqrp
      181 ggv
//
LOCUS       XP_077502607             182 aa            linear   INV 25-NOV-2025
DEFINITION  protein TMEPAI-like isoform X6 [Amblyomma americanum].
ACCESSION   XP_077502607
VERSION     XP_077502607.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646481.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..182
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..182
                     /product="protein TMEPAI-like isoform X6"
                     /calculated_mol_wt=19478
     CDS             1..182
                     /gene="LOC144113413"
                     /coded_by="XM_077646481.1:176..724"
                     /db_xref="GeneID:144113413"
ORIGIN      
        1 mlasswsqvv ssllssvfsl glvacivshy kmgarswser rsrpaphdpq pyqhvllppv
       61 qsalyssasa aerqraalaa ardaqvraas vrtdlalelp ptvalpeehp lagsvrlrda
      121 dqeqelygac vrappnrtvr dgsppcgggg rlrrvvvrsn svtpcnrtar whspdcqrpg
      181 gv
//
LOCUS       XP_077502608             583 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113414 [Amblyomma americanum].
ACCESSION   XP_077502608
VERSION     XP_077502608.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646482.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..583
                     /product="uncharacterized protein LOC144113414"
                     /calculated_mol_wt=62936
     Region          125..285
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; pfam01529"
                     /db_xref="CDD:396215"
     CDS             1..583
                     /gene="LOC144113414"
                     /coded_by="XM_077646482.1:427..2178"
                     /db_xref="GeneID:144113414"
ORIGIN      
        1 mkrdilqtrc ahllgvcqvl vdcgvkttkg gewsrqngws lplhplqlva wfflayflvm
       61 yfavlvpscp aggwqiglsl vnaavgivhl vshilsvtid padanvrakn ikgplpqfdr
      121 skhahvienq fcyicevkvg nrskhcsscn kciekfdhhc kwlnncvgsq nykyfavcva
      181 salmaclltf lvsaglivay faktglfhsl pvqrgnltvs avgsgedqsg sfyvfvpvdk
      241 tiwllmvevc aalsllaial lshllgfhlf lafkglstyd yivlkrnasf hggtreekkn
      301 fflkwckhkv nqvspvqqks savpkndnvd asgrtsvrid vpnghdktir gsslhsrdvq
      361 nnspsrqryi lreihksrlk varknrggkl mevsehgsha tavvglahqq gataspatap
      421 dpartkldsi slagaasasr svsqssldtd davqvpmaas aalslgtsaf ggslsnllfs
      481 estvlpaptp gellmehhsd sykespqear plvralpplt krtvvpatat vassadtege
      541 vftlssrlpe atdgnlqrwa vrsasipcgk tfevsadvvs dpr
//
LOCUS       XP_077502609             451 aa            linear   INV 25-NOV-2025
DEFINITION  transcription factor E2F5-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502609
VERSION     XP_077502609.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646483.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..451
                     /product="transcription factor E2F5-like isoform X1"
                     /calculated_mol_wt=48972
     Region          10..75
                     /region_name="E2F_TDP"
                     /note="E2F/DP family winged-helix DNA-binding domain;
                     pfam02319"
                     /db_xref="CDD:460530"
     Region          87..189
                     /region_name="E2F_DD"
                     /note="Dimerization domain of E2F transcription factors;
                     cl22411"
                     /db_xref="CDD:473951"
     Region          87..122
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:271137"
     Site            order(88..89,92..93,95..96,99..100,102..103,106..107,
                     109..110,112..114,116..117,120,128..133,136..137,140,
                     142..150,169..176,178..181,183,189)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:271137"
     Site            order(152..161,163..164,170)
                     /site_type="other"
                     /note="RbC binding site [polypeptide binding]"
                     /db_xref="CDD:271137"
     Region          <199..372
                     /region_name="PLN03237"
                     /note="DNA topoisomerase 2; Provisional"
                     /db_xref="CDD:215641"
     CDS             1..451
                     /gene="LOC144113416"
                     /coded_by="XM_077646483.1:70..1425"
                     /db_xref="GeneID:144113416"
ORIGIN      
        1 maeagapsrh ekslgllttk fvtllqeapd gvldlkvaad ilavrqkrri yditnvlegi
       61 gliekkskns iqwkgagpgc ntlelserlq vlqaeleele aaeatldehk awaqqslrni
      121 vedasnaara hittralrsc fpdstllslr gppdtvisvp dlrqtvdkny wlhakseqgp
      181 isvllldkel degsssllri ppevkapstt kikapadnaa svvtsaapee essvkqvple
      241 aqdiqmeevv sadseppdap sdresddden plppmpmtrr ksrqqqlpmk rklsdskapn
      301 gavkkakemp veapvtrmtt rssprssprk tllsaspqss plvtrsqraa aasvakvmet
      361 sdrsrlspke nagavsgnse psgntdvpkd laseelldlk emnlgdsdel yenmsptspf
      421 lclspplser dyffnldede gicdlfdilp v
//
LOCUS       XP_077502610             450 aa            linear   INV 25-NOV-2025
DEFINITION  transcription factor E2F5-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502610
VERSION     XP_077502610.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646484.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..450
                     /product="transcription factor E2F5-like isoform X2"
                     /calculated_mol_wt=48843
     Region          10..75
                     /region_name="E2F_TDP"
                     /note="E2F/DP family winged-helix DNA-binding domain;
                     pfam02319"
                     /db_xref="CDD:460530"
     Region          87..189
                     /region_name="E2F_DD"
                     /note="Dimerization domain of E2F transcription factors;
                     cl22411"
                     /db_xref="CDD:473951"
     Region          87..122
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:270870"
     Site            order(88..89,92..93,95..96,99..100,102..103,106..107,
                     109..110,112..114,116..117,120,128..133,136..137,140,
                     142..150,169..176,178..181,183,189)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:270870"
     Site            order(152..161,163..164,170)
                     /site_type="other"
                     /note="RbC binding site [polypeptide binding]"
                     /db_xref="CDD:270870"
     Region          <199..397
                     /region_name="PLN03237"
                     /note="DNA topoisomerase 2; Provisional"
                     /db_xref="CDD:215641"
     CDS             1..450
                     /gene="LOC144113416"
                     /coded_by="XM_077646484.1:70..1422"
                     /db_xref="GeneID:144113416"
ORIGIN      
        1 maeagapsrh ekslgllttk fvtllqeapd gvldlkvaad ilavrqkrri yditnvlegi
       61 gliekkskns iqwkgagpgc ntlelserlq vlqaeleele aaeatldehk awaqqslrni
      121 vedasnaara hittralrsc fpdstllslr gppdtvisvp dlrqtvdkny wlhakseqgp
      181 isvllldkel degsssllri ppevkapstt kikapadnaa svvtsaapee essvkqvple
      241 aqdiqmeevv sadseppdap sdresddden plppmpmtrr ksrqqqlpmk rklsdskapn
      301 gavkkakemp veapvtrmtt rssprssprk tllsaspqss plvtrsqraa aasvakvmet
      361 sdrsrlspkn agavsgnsep sgntdvpkdl aseelldlke mnlgdsdely enmsptspfl
      421 clspplserd yffnldedeg icdlfdilpv
//
LOCUS       XP_077502611             478 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113417 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502611
VERSION     XP_077502611.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646485.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..478
                     /product="uncharacterized protein LOC144113417 isoform X1"
                     /calculated_mol_wt=54114
     Region          29..125
                     /region_name="Nuf2"
                     /note="Nuf2 family; pfam03800"
                     /db_xref="CDD:461057"
     Region          <162..433
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..478
                     /gene="LOC144113417"
                     /coded_by="XM_077646485.1:222..1658"
                     /db_xref="GeneID:144113417"
ORIGIN      
        1 macraslapt akgnnneqdp lvemlercfn iratrqelda pasgliisil healvpfgln
       61 qealtvprfl lpseathies svplshyvkv mqdffcrlgi pdiglqdlan pkpkrtrkll
      121 kilvnswlrm taiyskykek ekkqleakki lhetkaassa rtaevnrlag llgaiageae
      181 kldkeqsace kalaascekk aaivsdyqql ktthyastev iqrteleiwe lkesveekra
      241 gvcrdpaark kkiaadtetl tslesavvdf qrklsvieek vklvpqirda lqkilttade
      301 ceakfdeime lreskeveak krikvehakh mlkkklelmg eeevrlgrrt iqphnaslry
      361 reqrnkdaet hyqelvehhk papaaedaet vvtatdgnlq pnspepdlqe eleaeqakle
      421 gaalrieeer dslqksldky iaknerrmci rleqvqdmvd rirdlwnpek lrslladl
//
LOCUS       XP_077502612             452 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113417 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502612
VERSION     XP_077502612.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646486.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..452
                     /product="uncharacterized protein LOC144113417 isoform X2"
                     /calculated_mol_wt=51335
     Region          29..125
                     /region_name="Nuf2"
                     /note="Nuf2 family; pfam03800"
                     /db_xref="CDD:461057"
     Region          <155..439
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..452
                     /gene="LOC144113417"
                     /coded_by="XM_077646486.1:222..1580"
                     /db_xref="GeneID:144113417"
ORIGIN      
        1 macraslapt akgnnneqdp lvemlercfn iratrqelda pasgliisil healvpfgln
       61 qealtvprfl lpseathies svplshyvkv mqdffcrlgi pdiglqdlan pkpkrtrkll
      121 kilvnswlrm taiyskykek ekkqleakki lhetkaassa rtaevnrlag llgaiageae
      181 kldkeqsace kalaascekk aaivsdyqql ktthyastev iqrteleiwe lkesveekra
      241 gvcrdpaark kkiaadtetl tslesavvdf qrklsvieek vklvpqirda lqkilttade
      301 ceakfdeime lreskeveak krikvehakh mlkkklelmg eeevrlgrrt iqphnaslry
      361 reqrnkdaet hyqelvehhk papaaedaeq aklegaalri eeerdslqks ldkyiakner
      421 rmcirleqvq dmvdrirdlw npeklrslla dl
//
LOCUS       XP_077502613             427 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113417 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502613
VERSION     XP_077502613.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646487.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..427
                     /product="uncharacterized protein LOC144113417 isoform X3"
                     /calculated_mol_wt=47856
     Region          29..125
                     /region_name="Nuf2"
                     /note="Nuf2 family; pfam03800"
                     /db_xref="CDD:461057"
     Region          <153..402
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..427
                     /gene="LOC144113417"
                     /coded_by="XM_077646487.1:221..1504"
                     /db_xref="GeneID:144113417"
ORIGIN      
        1 macraslapt akgnnneqdp lvemlercfn iratrqelda pasgliisil healvpfgln
       61 qealtvprfl lpseathies svplshyvkv mqdffcrlgi pdiglqdlan pkpkrtrkll
      121 kilvnswlrm taiyskykek ekkqleakki lhetkaassa rtaevnrlag llgaiageae
      181 kldkeqsace kalaascekk aaivsdyqql ktthyastev iqrteleiwe lkesveekra
      241 gvcrdpaark kkiaadtetl tslesavvdf qrklsvieek vklvpqirda lqkilttade
      301 ceakfdeime lreskeveak krikvehakh mlkkklelmg eeevrlgrrt iqphnaslry
      361 reqrnkdaet hyqelvehhk papaaedaet vvtatdgnlq pnspepdlqe eleaellpgr
      421 cqlvlaa
//
LOCUS       XP_077502614             417 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113417 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077502614
VERSION     XP_077502614.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646488.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..417
                     /product="uncharacterized protein LOC144113417 isoform X4"
                     /calculated_mol_wt=46835
     Region          29..125
                     /region_name="Nuf2"
                     /note="Nuf2 family; pfam03800"
                     /db_xref="CDD:461057"
     Region          <153..402
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..417
                     /gene="LOC144113417"
                     /coded_by="XM_077646488.1:221..1474"
                     /db_xref="GeneID:144113417"
ORIGIN      
        1 macraslapt akgnnneqdp lvemlercfn iratrqelda pasgliisil healvpfgln
       61 qealtvprfl lpseathies svplshyvkv mqdffcrlgi pdiglqdlan pkpkrtrkll
      121 kilvnswlrm taiyskykek ekkqleakki lhetkaassa rtaevnrlag llgaiageae
      181 kldkeqsace kalaascekk aaivsdyqql ktthyastev iqrteleiwe lkesveekra
      241 gvcrdpaark kkiaadtetl tslesavvdf qrklsvieek vklvpqirda lqkilttade
      301 ceakfdeime lreskeveak krikvehakh mlkkklelmg eeevrlgrrt iqphnaslry
      361 reqrnkdaet hyqelvehhk papaaedaet vvtatdgnlq pnspepdlqe eleaelt
//
LOCUS       XP_077502615             414 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113417 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077502615
VERSION     XP_077502615.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646489.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..414
                     /product="uncharacterized protein LOC144113417 isoform X5"
                     /calculated_mol_wt=46700
     Region          29..125
                     /region_name="Nuf2"
                     /note="Nuf2 family; pfam03800"
                     /db_xref="CDD:461057"
     Region          <184..345
                     /region_name="CCDC22"
                     /note="Coiled-coil domain-containing protein 22;
                     pfam05667"
                     /db_xref="CDD:461708"
     CDS             1..414
                     /gene="LOC144113417"
                     /coded_by="XM_077646489.1:222..1466"
                     /db_xref="GeneID:144113417"
ORIGIN      
        1 macraslapt akgnnneqdp lvemlercfn iratrqelda pasgliisil healvpfgln
       61 qealtvprfl lpseathies svplshyvkv mqdffcrlgi pdiglqdlan pkpkrtrkll
      121 kilvnswlrm taiyskykek ekkqleakki lhetkaassa rtaevnrlag llgaiageae
      181 kldkeqsace kalaascekk aaivsdyqql ktthyastev iqrteleiwe lkesveekra
      241 gvcrdpaark kkiaadtetl tslesavvdf qrklsvieek vklvpqirda lqkilttade
      301 ceakfdeime lreskeveak krikvehakh mlkkklelmg eeevrlgrrt iqphnaslry
      361 reqrnkdaet hyqelvehhk papaaedaec wfsakvlkye glgnenlgpr fsfl
//
LOCUS       XP_077502616             401 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113417 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077502616
VERSION     XP_077502616.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646490.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..401
                     /product="uncharacterized protein LOC144113417 isoform X6"
                     /calculated_mol_wt=45077
     Region          29..125
                     /region_name="Nuf2"
                     /note="Nuf2 family; pfam03800"
                     /db_xref="CDD:461057"
     Region          <153..380
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..401
                     /gene="LOC144113417"
                     /coded_by="XM_077646490.1:221..1426"
                     /db_xref="GeneID:144113417"
ORIGIN      
        1 macraslapt akgnnneqdp lvemlercfn iratrqelda pasgliisil healvpfgln
       61 qealtvprfl lpseathies svplshyvkv mqdffcrlgi pdiglqdlan pkpkrtrkll
      121 kilvnswlrm taiyskykek ekkqleakki lhetkaassa rtaevnrlag llgaiageae
      181 kldkeqsace kalaascekk aaivsdyqql ktthyastev iqrteleiwe lkesveekra
      241 gvcrdpaark kkiaadtetl tslesavvdf qrklsvieek vklvpqirda lqkilttade
      301 ceakfdeime lreskeveak krikvehakh mlkkklelmg eeevrlgrrt iqphnaslry
      361 reqrnkdaet hyqelvehhk papaaedael lpgrcqlvla a
//
LOCUS       XP_077502618             391 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113417 isoform X7 [Amblyomma
            americanum].
ACCESSION   XP_077502618
VERSION     XP_077502618.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646492.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..391
                     /product="uncharacterized protein LOC144113417 isoform X7"
                     /calculated_mol_wt=44056
     Region          29..125
                     /region_name="Nuf2"
                     /note="Nuf2 family; pfam03800"
                     /db_xref="CDD:461057"
     CDS             1..391
                     /gene="LOC144113417"
                     /coded_by="XM_077646492.1:221..1396"
                     /db_xref="GeneID:144113417"
ORIGIN      
        1 macraslapt akgnnneqdp lvemlercfn iratrqelda pasgliisil healvpfgln
       61 qealtvprfl lpseathies svplshyvkv mqdffcrlgi pdiglqdlan pkpkrtrkll
      121 kilvnswlrm taiyskykek ekkqleakki lhetkaassa rtaevnrlag llgaiageae
      181 kldkeqsace kalaascekk aaivsdyqql ktthyastev iqrteleiwe lkesveekra
      241 gvcrdpaark kkiaadtetl tslesavvdf qrklsvieek vklvpqirda lqkilttade
      301 ceakfdeime lreskeveak krikvehakh mlkkklelmg eeevrlgrrt iqphnaslry
      361 reqrnkdaet hyqelvehhk papaaedael t
//
LOCUS       XP_077502619             822 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113421 [Amblyomma americanum].
ACCESSION   XP_077502619
VERSION     XP_077502619.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646493.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..822
                     /product="uncharacterized protein LOC144113421"
                     /calculated_mol_wt=89165
     Region          108..128
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Site            order(115,117..118,122,127,158,161..165,167..168)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275371"
     Region          152..175
                     /region_name="zf-met"
                     /note="Zinc-finger of C2H2 type; pfam12874"
                     /db_xref="CDD:463736"
     Region          153..175
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Region          <192..346
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          224..246
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Region          224..246
                     /region_name="zf-met"
                     /note="Zinc-finger of C2H2 type; pfam12874"
                     /db_xref="CDD:463736"
     Region          349..373
                     /region_name="zf-met"
                     /note="Zinc-finger of C2H2 type; pfam12874"
                     /db_xref="CDD:463736"
     Region          351..373
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Region          445..469
                     /region_name="zf-met"
                     /note="Zinc-finger of C2H2 type; pfam12874"
                     /db_xref="CDD:463736"
     CDS             1..822
                     /gene="LOC144113421"
                     /coded_by="XM_077646493.1:108..2576"
                     /db_xref="GeneID:144113421"
ORIGIN      
        1 mstevvsegs aveecdvgen vdftaddtes kswddvpfdq tplensheev knedivaada
       61 eagaveveee qhaeiteaps edvnkeladp qaeessaven esepsytcdm cqgtfeetee
      121 ehlkgrkhlk lmerlekygs leaiaaefqp stcylcnvss vsksqmqmhl sgnkhkarca
      181 slnlhpsamd fpgknrkpqt pavtdaktpg pldpnsivte rpacdvcnit lssvpqlmqh
      241 ltgkrhkelv talklavmrl ppnlrppqlr mpprkndgkp psgpttkpap vpkpgvavqg
      301 ppaqalkrpg ppadgptavp akkakldsnp eegsavqktp ggkpaarkfs cdicnirlns
      361 efqrnehlkg kihkdrlael kgkpggtvkp agtikaagtv kpagpvlqkg allgnkppga
      421 plgkqgsvpk mksqqqqkln atasfwcdvc qvglssqlhl nqhlgsnrhc enfaryggng
      481 pslfaqagkk vpggaqrapn lqgfapresq nwagppppnn anghfrtpap ptqhqssvwq
      541 tpsvqpqwgg pvlptpapsf snqrsseepl sssfglleps aqanreqgay glntsqhfsk
      601 lqldlaleis klsqhlarpe fsqelsqqls hhlsqelsrq rqmatdrdrs tredfgtter
      661 lpllgdglrl rgegapllgd glrlrgegap llgdglrlrg egaplldegp llrdkaplir
      721 dsfedraprr ppllgesgpt fssreqdysf errddfdrrh gidsygerrn hdfvdsyntd
      781 ygsnhgyddl pfggsadfqr kprssaggll drpplldshs rf
//
LOCUS       XP_077502620             575 aa            linear   INV 25-NOV-2025
DEFINITION  eukaryotic translation initiation factor 2-alpha kinase 1-like
            [Amblyomma americanum].
ACCESSION   XP_077502620
VERSION     XP_077502620.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646494.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..575
                     /product="eukaryotic translation initiation factor 2-alpha
                     kinase 1-like"
                     /calculated_mol_wt=64160
     Region          142..535
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(155..158,161,163,176,178,209,370..373,426,430..431,
                     433,444..445)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..575
                     /gene="LOC144113423"
                     /coded_by="XM_077646494.1:273..2000"
                     /db_xref="GeneID:144113423"
ORIGIN      
        1 mdppipklsl rtvttldvpd igrppprlvg qesfglvprg qipyylvles vleqlcifye
       61 sdlslrkelf egvrqrlhql kfldptpdlq elcslrvkfr hsfsfliqnv rrdlrrrvsg
      121 slvclpgpql draldnevic hqkryeydfe eiekiaeggf givckamrrt drqiyavkki
      181 pfkycnlkvl kqvlrevell aqlshpniva yksawienvt dfmlkshaat vdscqstvli
      241 ssgsgdnkgs ppysvdlsss qeivpaschk slspntstea iyfmdsgtsv teqcasleep
      301 skltmpsaee lnrspynsks dselgkgndg haangrkass hvtnspavvt pdifrllnss
      361 dvggmlyiqm elcsknladw latrnqqlae fgesgdsgti pkamhifkqi lkgveymhsk
      421 gfihrdikpq nilfdlegsf vklgdfglat rsnqqepssh pfpwtprsgh tqgvgtslya
      481 apeqceqacy dskvdiyslg vvltellcpf steherltel tklrngsipt aleahsedvv
      541 cylfctqslm vflplmdlkn vsifidllih lfrta
//
LOCUS       XP_077502621             575 aa            linear   INV 25-NOV-2025
DEFINITION  eukaryotic translation initiation factor 2-alpha kinase 1-like
            [Amblyomma americanum].
ACCESSION   XP_077502621
VERSION     XP_077502621.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646495.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..575
                     /product="eukaryotic translation initiation factor 2-alpha
                     kinase 1-like"
                     /calculated_mol_wt=64160
     Region          142..535
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(155..158,161,163,176,178,209,370..373,426,430..431,
                     433,444..445)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..575
                     /gene="LOC144113423"
                     /coded_by="XM_077646495.1:213..1940"
                     /db_xref="GeneID:144113423"
ORIGIN      
        1 mdppipklsl rtvttldvpd igrppprlvg qesfglvprg qipyylvles vleqlcifye
       61 sdlslrkelf egvrqrlhql kfldptpdlq elcslrvkfr hsfsfliqnv rrdlrrrvsg
      121 slvclpgpql draldnevic hqkryeydfe eiekiaeggf givckamrrt drqiyavkki
      181 pfkycnlkvl kqvlrevell aqlshpniva yksawienvt dfmlkshaat vdscqstvli
      241 ssgsgdnkgs ppysvdlsss qeivpaschk slspntstea iyfmdsgtsv teqcasleep
      301 skltmpsaee lnrspynsks dselgkgndg haangrkass hvtnspavvt pdifrllnss
      361 dvggmlyiqm elcsknladw latrnqqlae fgesgdsgti pkamhifkqi lkgveymhsk
      421 gfihrdikpq nilfdlegsf vklgdfglat rsnqqepssh pfpwtprsgh tqgvgtslya
      481 apeqceqacy dskvdiyslg vvltellcpf steherltel tklrngsipt aleahsedvv
      541 cylfctqslm vflplmdlkn vsifidllih lfrta
//
LOCUS       XP_077502622             575 aa            linear   INV 25-NOV-2025
DEFINITION  eukaryotic translation initiation factor 2-alpha kinase 1-like
            [Amblyomma americanum].
ACCESSION   XP_077502622
VERSION     XP_077502622.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646496.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..575
                     /product="eukaryotic translation initiation factor 2-alpha
                     kinase 1-like"
                     /calculated_mol_wt=64160
     Region          142..535
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(155..158,161,163,176,178,209,370..373,426,430..431,
                     433,444..445)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..575
                     /gene="LOC144113423"
                     /coded_by="XM_077646496.1:204..1931"
                     /db_xref="GeneID:144113423"
ORIGIN      
        1 mdppipklsl rtvttldvpd igrppprlvg qesfglvprg qipyylvles vleqlcifye
       61 sdlslrkelf egvrqrlhql kfldptpdlq elcslrvkfr hsfsfliqnv rrdlrrrvsg
      121 slvclpgpql draldnevic hqkryeydfe eiekiaeggf givckamrrt drqiyavkki
      181 pfkycnlkvl kqvlrevell aqlshpniva yksawienvt dfmlkshaat vdscqstvli
      241 ssgsgdnkgs ppysvdlsss qeivpaschk slspntstea iyfmdsgtsv teqcasleep
      301 skltmpsaee lnrspynsks dselgkgndg haangrkass hvtnspavvt pdifrllnss
      361 dvggmlyiqm elcsknladw latrnqqlae fgesgdsgti pkamhifkqi lkgveymhsk
      421 gfihrdikpq nilfdlegsf vklgdfglat rsnqqepssh pfpwtprsgh tqgvgtslya
      481 apeqceqacy dskvdiyslg vvltellcpf steherltel tklrngsipt aleahsedvv
      541 cylfctqslm vflplmdlkn vsifidllih lfrta
//
LOCUS       XP_077502623             198 aa            linear   INV 25-NOV-2025
DEFINITION  fibroblast growth factor 17-like [Amblyomma americanum].
ACCESSION   XP_077502623
VERSION     XP_077502623.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646497.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..198
                     /product="fibroblast growth factor 17-like"
                     /calculated_mol_wt=22335
     Region          51..170
                     /region_name="beta-trefoil_FGF8-like"
                     /note="FGF domain, beta-trefoil fold, found in the
                     fibroblast growth factor 8 (FGF8)-like family; cd23307"
                     /db_xref="CDD:466993"
     CDS             1..198
                     /gene="LOC144113424"
                     /coded_by="XM_077646497.1:230..826"
                     /db_xref="GeneID:144113424"
ORIGIN      
        1 mlihkfvvpi tltllvslvm vkcqgfgskn knlelvgdvp sgrsiqneff lfnkcskgni
       61 llkgkkvtaa pgasssklvl vsvgmgsklt ihsngtflcf nkggrlvnkr rrsrrkslce
      121 freilrdnys tfqsvfnsnw yigfnrsgqp irggrkvrer kwasymflkr elqkqrpdpf
      181 gkvpsvewyk hlnatdss
//
LOCUS       XP_077502624             774 aa            linear   INV 25-NOV-2025
DEFINITION  junctophilin [Amblyomma americanum].
ACCESSION   XP_077502624
VERSION     XP_077502624.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646498.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..774
                     /product="junctophilin"
                     /calculated_mol_wt=84389
     Region          <2..133
                     /region_name="COG4642"
                     /note="Uncharacterized conserved protein [Function
                     unknown]"
                     /db_xref="CDD:443680"
     Region          99..>349
                     /region_name="PLN03185"
                     /note="phosphatidylinositol phosphate kinase; Provisional"
                     /db_xref="CDD:215619"
     CDS             1..774
                     /gene="jp"
                     /coded_by="XM_077646498.1:179..2503"
                     /db_xref="GeneID:144113426"
ORIGIN      
        1 msggrfdfdd ggtycggwed gkahghgvct gpkgqgeycg swhygfevsg vytwpsgsgy
       61 egqwqngkrh glgvetrgrw ryrgewtqgf kgrygvrqsl isgakyegtw anglqdgygs
      121 etyadggtyq gqwlrgtrhg ygvrssapfg laahnrnpss ggkvevrtsq tsldssshpv
      181 dpaserdrki ddcrggfvlk arsdeppqrr rslvekssnf kksilqglri kkqhstgdid
      241 krgvssgssl rssgsnmssa ssesaqsglt eahtdsnasf vsqedmtdqn vmetymgewk
      301 ndrrsgygia ersdglkyeg ewynnkkygy gvttfkdgar eegkyknnll vtsgkkkhlf
      361 llrsakfrer veaavaaalr asqialqkad iaisrtatar gkaeqadvaa lhakddaeva
      421 rsvskqyape lytpvdllkk laevpdlakl rvaaggatpq rrrslfqptr aqeltapqld
      481 aaqqqllnal tkahqqskpg gsrqgtppva nakpapplqp apqqapvqhv qavslpqqlp
      541 qridegmpqv pgapeppprt phgslhgrqt qrdgysfqqa mndhfeqyrr erppsrtpph
      601 kadqqlpplt aiyggadctp dsgvsesmdg dssptrlpgg ggsggrrstl pnmpadegsh
      661 hraaslyvaa prrrlaapap aesltrkksl pniadaaaaa aapafmsree vsvlssqrrd
      721 elrrmqeqah rlrtnpllyl fnsdvkdwfs rqqlvmlvlf inislaimff kllt
//
LOCUS       XP_077502625             449 aa            linear   INV 25-NOV-2025
DEFINITION  CCR4-NOT transcription complex subunit 11 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502625
VERSION     XP_077502625.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646499.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..449
                     /product="CCR4-NOT transcription complex subunit 11
                     isoform X1"
                     /calculated_mol_wt=50524
     Region          307..430
                     /region_name="CNOT11"
                     /note="CCR4-NOT transcription complex subunit 11;
                     pfam10155"
                     /db_xref="CDD:401964"
     CDS             1..449
                     /gene="Not11"
                     /coded_by="XM_077646499.1:232..1581"
                     /db_xref="GeneID:144113427"
ORIGIN      
        1 maltekelgl llsllqddql ekqtfeslgq tlqhhfakqd hfrvscalal liqqsdlisg
       61 pcqrivalyf lyemyrtesi hmnpfisifv hllnpaeetg gkkpefahvi pkltvhekyf
      121 ltqlltvpak dlfkktpwqv mnldesclqm gdtggiqvsf aehqsempqs srsgiplvid
      181 dpdlrrpiig gdapspakam qqlltgenpp vegvfqpefl rlvpplhdcd geltwitpfe
      241 hgdysllwep ticqsdtagp eakrlmaraf kstltqpqqq qlldelaadp klvyhiglsp
      301 aklpelvenn plvaievllk lmqssqitey fsvlvnmems lhsmevvnrl ttavelptef
      361 vhlyisncis tcetikdrym qnrlvrlvcv flqslirnki invqdlfiev qafciefsri
      421 reaaalfrll kqldtaeqgp sspsqasak
//
LOCUS       XP_077502626             444 aa            linear   INV 25-NOV-2025
DEFINITION  CCR4-NOT transcription complex subunit 11 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502626
VERSION     XP_077502626.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646500.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..444
                     /product="CCR4-NOT transcription complex subunit 11
                     isoform X2"
                     /calculated_mol_wt=50050
     Region          302..425
                     /region_name="CNOT11"
                     /note="CCR4-NOT transcription complex subunit 11;
                     pfam10155"
                     /db_xref="CDD:401964"
     CDS             1..444
                     /gene="Not11"
                     /coded_by="XM_077646500.1:231..1565"
                     /db_xref="GeneID:144113427"
ORIGIN      
        1 maltekelgl llsllqddql ekqtfeslgq tlqhhfakqd hfralliqqs dlisgpcqri
       61 valyflyemy rtesihmnpf isifvhllnp aeetggkkpe fahvipkltv hekyfltqll
      121 tvpakdlfkk tpwqvmnlde sclqmgdtgg iqvsfaehqs empqssrsgi plviddpdlr
      181 rpiiggdaps pakamqqllt genppvegvf qpeflrlvpp lhdcdgeltw itpfehgdys
      241 llwepticqs dtagpeakrl marafkstlt qpqqqqllde laadpklvyh iglspaklpe
      301 lvennplvai evllklmqss qiteyfsvlv nmemslhsme vvnrlttave lptefvhlyi
      361 sncistceti kdrymqnrlv rlvcvflqsl irnkiinvqd lfievqafci efsrireaaa
      421 lfrllkqldt aeqgpsspsq asak
//
LOCUS       XP_077502627            1044 aa            linear   INV 25-NOV-2025
DEFINITION  nuclear factor of activated T cells 3 [Amblyomma americanum].
ACCESSION   XP_077502627
VERSION     XP_077502627.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646501.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (GKSP01013738.1) annotated
            using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 771 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..1044
                     /product="nuclear factor of activated T cells 3"
                     /calculated_mol_wt=111102
     Region          288..457
                     /region_name="RHD-n"
                     /note="N-terminal sub-domain of the Rel homology domain
                     (RHD); cl08275"
                     /db_xref="CDD:447596"
     Site            order(301,303..304,306..311,397,399..400,457)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143642"
     Region          461..566
                     /region_name="IPT"
                     /note="Immunoglobulin-like fold, Plexins, Transcription
                     factors (IPT). IPTs are also known as Transcription factor
                     ImmunoGlobin (TIG) domains. They are present in
                     intracellular transcription factors, cell surface
                     receptors (such as plexins and scatter factor...; cl15674"
                     /db_xref="CDD:472823"
     CDS             1..1044
                     /gene="NFAT"
                     /coded_by="XM_077646501.1:222..3356"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 771 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144113428"
ORIGIN      
        1 mgkitnrpqa dtsnlssgyi skgpvvplgp assgakeery glrsgqrvsq dpvssyrdlp
       61 yselselasa lceqnhrmes nlstldctld ttnqhedsvs stvrdiaqds gsllqepnsm
      121 lvrmlhmtvk eeqswlgalg gspacsgsps ssrasasppp qspcralaga splavseeat
      181 lgdapqpset qlasragpdf hnlqtalral ikevtqaspq eqhctpasic gvaksllsad
      241 sssnrssglf appvsmvtkg gitrrvnqva krptlymsfp srsrrgdvel kilsqpeeqh
      301 raryltegsr gavkdrtgmg fptvklaglq eqvklqvfig tdqgkvqphm fyqasrvcgk
      361 nstpcqerrl dgttvleidm lpskdmtvsc dcvgilkern vdvekrlvry ggtrnkkrst
      421 kcrlvfrvvl paqqeqdsgg lpseilqvas apilctqppg ipeiskksls ecsikgevel
      481 fiigknflkd tkvlfkepas dnlgdcepny vwekcvipek eylqpthlic rvpayrnpdv
      541 ghlvvvhlvv tsggraseph pftyvplagg gqiptgplsa ttakssanaa aasslelktq
      601 pfggsqgaql ptiiqiplla gtsprsqrhp vqtlvipttt ialspasasv pesqpkpgns
      661 sgaavaaaat snniqfvvvq qpaqqptqsq vqqqqaqqsq qpaapatlsl vvssaaqqtl
      721 lkaaeeaasp aneschlkrk ishapegapd kcaanyelsg eeplknteeq lgnlglfytd
      781 qgilpscsve qlmgvtqeag ttactaatsa tfecetqwst lthqsqpenk plwgqmgces
      841 ataeselegr ppemqslent avpfadeqqf kkqkvkdapv lslpswptgs leqvqsmdft
      901 csgvkpevap swpnslngml sqpepkvpvv fgdkvildsv gvstapssqf svtgsfennp
      961 qcqdnmaaga fgitsfeaat qpvsqpqalv vepslvvsrd qasssaqqaq tqaqgpnmdh
     1021 isamsmlpes ellrminpna fdnv
//
LOCUS       XP_077502628             287 aa            linear   INV 25-NOV-2025
DEFINITION  homeobox protein Nkx-2.5-like [Amblyomma americanum].
ACCESSION   XP_077502628
VERSION     XP_077502628.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646502.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..287
                     /product="homeobox protein Nkx-2.5-like"
                     /calculated_mol_wt=30875
     Region          150..200
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..287
                     /gene="LOC144113430"
                     /coded_by="XM_077646502.1:60..923"
                     /db_xref="GeneID:144113430"
ORIGIN      
        1 mlassatpfs vkdilnlsds gvdeldpqal lyqaqsleka ffeeachegl ldpdalllpa
       61 eaaataaatg aggqsaphlc lpyhaacfdg aglepayaqq hdegppphla qhcttgrpdl
      121 lgqqfvndak sggrecgprp rkrkkprvlf sqsqvfeleq rfkqqkylsa perdrlanal
      181 kltstqvkiw fqnrrykckr qtqdkslemv aaasarrvav pvlvrdgkpa vpfpppapsy
      241 pmhpyfagat alappppapp qppdasalmy pamgghrpta tgairtw
//
LOCUS       XP_077502629             502 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113431 [Amblyomma americanum].
ACCESSION   XP_077502629
VERSION     XP_077502629.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646503.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..502
                     /product="uncharacterized protein LOC144113431"
                     /calculated_mol_wt=54428
     Region          266..502
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            272..279
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206648"
     Site            order(274..280,324,395..396,398,425..427)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206648"
     Site            296
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206648"
     Site            300..302
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206648"
     Site            321..324
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206648"
     Site            order(323..324,355..356)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206648"
     Site            395..398
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206648"
     Site            425..427
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206648"
     CDS             1..502
                     /gene="LOC144113431"
                     /coded_by="XM_077646503.1:248..1756"
                     /db_xref="GeneID:144113431"
ORIGIN      
        1 malenaarra sptratarps ssqgsadsen cltgdgaggg aaaggrspss qqqhspshsv
       61 rrkprasphr yhgshsngca satasplrda svsrsqsmrt trrphslqrh frdriasipg
      121 dllmvngand srgssssslp cdeptadvqr lrnfavtskg vvnrgdsfrs ksrsthsvas
      181 tgsghklavp aaadgeggda ycvcasldgd rrvvsslqql spepaartdr asfalaclep
      241 agpslsglpp evvvtrsaea adlaryrilv lgarevgkts linqfmtsey iyaydssvde
      301 eneksvmvvl ngeeselifi ehahqdellq htrnpcqalr tpptlfnpaa myrpdaylvv
      361 ynvcsrsslk iakeylslih rwdnvdhkav imvgnktdlv rhrtvttddg rclatnervk
      421 fietsacinh hvdellagll hqirlknqis ykesckrdsv rmrgrnstct ssfsgckakv
      481 flkrflrkac srsrscddlh vl
//
LOCUS       XP_077502630            1674 aa            linear   INV 25-NOV-2025
DEFINITION  A.superbus venom factor 1-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502630
VERSION     XP_077502630.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646504.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1674
                     /product="A.superbus venom factor 1-like isoform X1"
                     /calculated_mol_wt=187502
     Region          26..122
                     /region_name="MG1"
                     /note="Macroglobulin domain MG1; pfam17790"
                     /db_xref="CDD:465508"
     Region          <123..1339
                     /region_name="YfaS"
                     /note="Uncharacterized conserved protein YfaS,
                     alpha-2-macroglobulin family [General function prediction
                     only]; COG2373"
                     /db_xref="CDD:441940"
     Region          668..741
                     /region_name="ANATO"
                     /note="Anaphylatoxin homologous domain; C3a, C4a and C5a
                     anaphylatoxins are protein fragments generated
                     enzymatically in serum during activation of complement
                     molecules C3, C4, and C5. They induce smooth muscle
                     contraction. These fragments are homologous to...;
                     cd00017"
                     /db_xref="CDD:237984"
     Region          1004..1272
                     /region_name="A2M_like"
                     /note="Proteins similar to alpha2-macroglobulin (alpha
                     (2)-M). Alpha (2)-M is a major carrier protein in serum.
                     It is a broadly specific proteinase inhibitor. The
                     structural thioester of alpha (2)-M, is involved in the
                     immobilization and entrapment of...; cd02891"
                     /db_xref="CDD:239221"
     Site            order(1008,1010..1011,1014,1054,1064,1066,1256)
                     /site_type="other"
                     /note="surface patch"
                     /db_xref="CDD:239221"
     Site            1008..1011
                     /site_type="active"
                     /note="thioester region [active]"
                     /db_xref="CDD:239221"
     Site            order(1106,1110,1112)
                     /site_type="other"
                     /note="specificity defining residues"
                     /db_xref="CDD:239221"
     Region          1405..1493
                     /region_name="A2M_recep"
                     /note="A-macroglobulin receptor binding domain; pfam07677"
                     /db_xref="CDD:462226"
     Region          1548..1656
                     /region_name="NTR"
                     /note="UNC-6/NTR/C345C module; pfam01759"
                     /db_xref="CDD:396359"
     CDS             1..1674
                     /gene="LOC144113433"
                     /coded_by="XM_077646504.1:105..5129"
                     /db_xref="GeneID:144113433"
ORIGIN      
        1 mastlalvca lfvalctprm saeencfvva pnvfrlgtne tfavmvdgvk krvtvtlqny
       61 pssrgaffhw tgdvasdnlr ivdiavketd lpelfygrdq iyvtlevtcg rlwtrktqvl
      121 vspasgehff lqtekpiyhp gstvnirfla vdgklepsas sfrlevrnpq nvivertdfq
      181 pskelmltht yelpertllg ewalvvkygy kfqqnttatf lvdkyvlprf kvdlsvpdyv
      241 lsnfstipcr vkasfvnkmp vhgvvllefg lrhevgdiww fssskspkll eggeahyilr
      301 realepnigd klpdlhathg rlvvkatvte eatgakesaq sektvfcktp fvvslkknqr
      361 sfkpglkiyi taevtyvngn paanvltkat rlnsrdtfta rtrsdgvatf lipvsfedgt
      421 lsvkvetads ryrpehqara qislepystv qsgfiaierk dpksfprard tyeavlfthr
      481 fdkvsssiyy vvlskgriql vdkllegrri eqniafsvtp emtpsfrvvv faildgrvvt
      541 dsiyvnaepa ctttsqftle rrnlgnppep msqetlvltg trgtvvgllg vdqamyllrk
      601 kdlltrdklf rsldskdlgc gagggtnaae alyssgivll thedvgnpmr sgvgcheerr
      661 rrrrdaqnil eeyedetvrk cclrgqwpdr flrscskrag ilrkymeqgs anitqdcvea
      721 ferccdiaeh daaqrsssag vdelsdeylg vhnqirddfr etwifhsmti rddgtaefsa
      781 tlpasvttwe vnavsvspsg gvcaveplei vatkkffvev nvpysvvkke qveipatvyn
      841 ygtkqitakv vllgtndics gaklgkpsav rvleippghg rtaifpvvpl aagkkqihvk
      901 arstsgegde vkvelnvrpp gvqrtrsfav ildpenpkkr ntrniqeeyt etfgtngtqv
      961 vhirsprpdf alpntercei divgdgvtav lqsiikkpdq afiypsdcge qttaklmpvl
     1021 yayeffktan risiaeenda ldyirraynq ilkyrkpdgs fsvfewssas pwltafvirn
     1081 lceatksvmi denvirsglr yitsrqqgng gfheyslsel vlnfghpsal tafilitfee
     1141 caeggysvsq tsraraaafl ernlhrgdsp galalaayal slanntgkdg liqwlmesvr
     1201 ydqvtddrhv passellsaq atayalmali renaqseiir sfqrwlskrt gptgsvqstq
     1261 dtvmvlqalt kfaayakenn ldlacevtls nskifkknir ikrdnatiln kieidrpgek
     1321 ifvrvkgsgt gilyfnytyd akvldgelck fnitanfvet kpdieliltr vsrsvektss
     1381 rppsdlkpny rmeacarpve hapdgmvmle vglltgfkpn ateldelvke rkiqlytits
     1441 rhvdfyvpfi aanmthcvnf sleqefnagk lqssyvkays yykpdfsctr fyspdktspl
     1501 lkadkcddsd vcvcaeggcp pekpldrfik irddeyfede eqrellrefa cdgvhyvwrg
     1561 nstanvstdg fievaflitq vlkpgqeddl kgkirrikar dtcntfnipn geeyivmgkd
     1621 styiekdlfe tkqflylids ssmvfpakpk nkqqnrlaaw finefsdert rcfl
//
LOCUS       XP_077502631            1656 aa            linear   INV 25-NOV-2025
DEFINITION  A.superbus venom factor 1-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502631
VERSION     XP_077502631.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646505.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1656
                     /product="A.superbus venom factor 1-like isoform X2"
                     /calculated_mol_wt=185298
     Region          26..122
                     /region_name="MG1"
                     /note="Macroglobulin domain MG1; pfam17790"
                     /db_xref="CDD:465508"
     Region          <123..1321
                     /region_name="YfaS"
                     /note="Uncharacterized conserved protein YfaS,
                     alpha-2-macroglobulin family [General function prediction
                     only]; COG2373"
                     /db_xref="CDD:441940"
     Region          986..1254
                     /region_name="A2M_like"
                     /note="Proteins similar to alpha2-macroglobulin (alpha
                     (2)-M). Alpha (2)-M is a major carrier protein in serum.
                     It is a broadly specific proteinase inhibitor. The
                     structural thioester of alpha (2)-M, is involved in the
                     immobilization and entrapment of...; cd02891"
                     /db_xref="CDD:239221"
     Site            order(990,992..993,996,1036,1046,1048,1238)
                     /site_type="other"
                     /note="surface patch"
                     /db_xref="CDD:239221"
     Site            990..993
                     /site_type="active"
                     /note="thioester region [active]"
                     /db_xref="CDD:239221"
     Site            order(1088,1092,1094)
                     /site_type="other"
                     /note="specificity defining residues"
                     /db_xref="CDD:239221"
     Region          1387..1475
                     /region_name="A2M_recep"
                     /note="A-macroglobulin receptor binding domain; pfam07677"
                     /db_xref="CDD:462226"
     Region          1530..1638
                     /region_name="NTR"
                     /note="UNC-6/NTR/C345C module; pfam01759"
                     /db_xref="CDD:396359"
     CDS             1..1656
                     /gene="LOC144113433"
                     /coded_by="XM_077646505.1:87..5057"
                     /db_xref="GeneID:144113433"
ORIGIN      
        1 mastlalvca lfvalctprm saeencfvva pnvfrlgtne tfavmvdgvk krvtvtlqny
       61 pssrgaffhw tgdvasdnlr ivdiavketd lpelfygrdq iyvtlevtcg rlwtrktqvl
      121 vspasgehff lqtekpiyhp gstvnirfla vdgklepsas sfrlevrnpq nvivertdfq
      181 pskelmltht yelpertllg ewalvvkygy kfqqnttatf lvdkyvlprf kvdlsvpdyv
      241 lsnfstipcr vkasfvnkmp vhgvvllefg lrhevgdiww fssskspkll eggeahyilr
      301 realepnigd klpdlhathg rlvvkatvte eatgakesaq sektvfcktp fvvslkknqr
      361 sfkpglkiyi taevtyvngn paanvltkat rlnsrdtfta rtrsdgvatf lipvsfedgt
      421 lsvkvetads ryrpehqara qislepystv qsgfiaierk dpksfprard tyeavlfthr
      481 fdkvsssiyy vvlskgriql vdkllegrri eqniafsvtp emtpsfrvvv faildgrvvt
      541 dsiyvnaepa ctttsqftle rrnlgnppep msqetlvltg trgtvvgllg vdqamyllrk
      601 kdlltrdklf rsldskdlgc gagggtnaae alyssgivll thedvgnpmr sveeyedetv
      661 rkcclrgqwp drflrscskr agilrkymeq gsanitqdcv eaferccdia ehdaaqrsss
      721 agvdelsdey lgvhnqirdd fretwifhsm tirddgtaef satlpasvtt wevnavsvsp
      781 sggvcavepl eivatkkffv evnvpysvvk keqveipatv ynygtkqita kvvllgtndi
      841 csgaklgkps avrvleippg hgrtaifpvv plaagkkqih vkarstsgeg devkvelnvr
      901 ppgvqrtrsf avildpenpk krntrniqee ytetfgtngt qvvhirsprp dfalpnterc
      961 eidivgdgvt avlqsiikkp dqafiypsdc geqttaklmp vlyayeffkt anrisiaeen
     1021 daldyirray nqilkyrkpd gsfsvfewss aspwltafvi rnlceatksv midenvirsg
     1081 lryitsrqqg nggfheysls elvlnfghps altafilitf eecaeggysv sqtsraraaa
     1141 flernlhrgd spgalalaay alslanntgk dgliqwlmes vrydqvtddr hvpassells
     1201 aqatayalma lirenaqsei irsfqrwlsk rtgptgsvqs tqdtvmvlqa ltkfaayake
     1261 nnldlacevt lsnskifkkn irikrdnati lnkieidrpg ekifvrvkgs gtgilyfnyt
     1321 ydakvldgel ckfnitanfv etkpdielil trvsrsvekt ssrppsdlkp nyrmeacarp
     1381 vehapdgmvm levglltgfk pnateldelv kerkiqlyti tsrhvdfyvp fiaanmthcv
     1441 nfsleqefna gklqssyvka ysyykpdfsc trfyspdkts pllkadkcdd sdvcvcaegg
     1501 cppekpldrf ikirddeyfe deeqrellre facdgvhyvw rgnstanvst dgfievafli
     1561 tqvlkpgqed dlkgkirrik ardtcntfni pngeeyivmg kdstyiekdl fetkqflyli
     1621 dsssmvfpak pknkqqnrla awfinefsde rtrcfl
//
LOCUS       XP_077502632             153 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial ribosomal protein L52 [Amblyomma americanum].
ACCESSION   XP_077502632
VERSION     XP_077502632.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646506.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..153
                     /product="mitochondrial ribosomal protein L52"
                     /calculated_mol_wt=17571
     Region          44..135
                     /region_name="MRPL52"
                     /note="Mitoribosomal protein mL52; pfam18699"
                     /db_xref="CDD:465836"
     CDS             1..153
                     /gene="mRpL52"
                     /coded_by="XM_077646506.1:92..553"
                     /db_xref="GeneID:144113434"
ORIGIN      
        1 maalmcgrvf erlkilrrna akglvvqpsr gastkevksr sllrdyhwrt afglprrwne
       61 selfadtpdw tfndgrptpl ngkqkkryle qleycativk ylkeinnaqe maaqvaqene
      121 elkkrvasgk fkqkgiqelq lhrpnqagrr aet
//
LOCUS       XP_077502633             153 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial ribosomal protein L52 [Amblyomma americanum].
ACCESSION   XP_077502633
VERSION     XP_077502633.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646507.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..153
                     /product="mitochondrial ribosomal protein L52"
                     /calculated_mol_wt=17571
     Region          44..135
                     /region_name="MRPL52"
                     /note="Mitoribosomal protein mL52; pfam18699"
                     /db_xref="CDD:465836"
     CDS             1..153
                     /gene="mRpL52"
                     /coded_by="XM_077646507.1:92..553"
                     /db_xref="GeneID:144113434"
ORIGIN      
        1 maalmcgrvf erlkilrrna akglvvqpsr gastkevksr sllrdyhwrt afglprrwne
       61 selfadtpdw tfndgrptpl ngkqkkryle qleycativk ylkeinnaqe maaqvaqene
      121 elkkrvasgk fkqkgiqelq lhrpnqagrr aet
//
LOCUS       XP_077502634             132 aa            linear   INV 25-NOV-2025
DEFINITION  GEL complex subunit OPTI isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502634
VERSION     XP_077502634.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646508.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..132
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..132
                     /product="GEL complex subunit OPTI isoform X1"
                     /calculated_mol_wt=14937
     Region          41..114
                     /region_name="EMC6"
                     /note="pfam07019"
                     /db_xref="CDD:462067"
     CDS             1..132
                     /gene="LOC144113436"
                     /coded_by="XM_077646508.1:134..532"
                     /db_xref="GeneID:144113436"
ORIGIN      
        1 mnqrrkqaer nghakgslss vwvraftpns ewpdkdefld vlywsrqilg illglawglv
       61 plkgflgial ffcinvvlvy gytssfqkvd deeyggvwel vkegfvtsfa gflvptlrrf
      121 hgypeaknmg mr
//
LOCUS       XP_077502635             126 aa            linear   INV 25-NOV-2025
DEFINITION  GEL complex subunit OPTI isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502635
VERSION     XP_077502635.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646509.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..126
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..126
                     /product="GEL complex subunit OPTI isoform X2"
                     /calculated_mol_wt=14297
     Region          41..117
                     /region_name="EMC6"
                     /note="pfam07019"
                     /db_xref="CDD:462067"
     CDS             1..126
                     /gene="LOC144113436"
                     /coded_by="XM_077646509.1:132..512"
                     /db_xref="GeneID:144113436"
ORIGIN      
        1 mnqrrkqaer nghakgslss vwvraftpns ewpdkdefld vlywsrqilg illglawglv
       61 plkgflgial ffcinvvlvy gytssfqkvd deeyggvwel vkegfvtsfa gflvtwimiy
      121 samqyd
//
LOCUS       XP_077502637             472 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113437 [Amblyomma americanum].
ACCESSION   XP_077502637
VERSION     XP_077502637.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646511.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..472
                     /product="uncharacterized protein LOC144113437"
                     /calculated_mol_wt=51301
     Region          <390..431
                     /region_name="EB"
                     /note="EB module; pfam01683"
                     /db_xref="CDD:460294"
     CDS             1..472
                     /gene="LOC144113437"
                     /coded_by="XM_077646511.1:320..1738"
                     /db_xref="GeneID:144113437"
ORIGIN      
        1 maklptllll vaaaavplaa vadaptttqp aggprhdgsg gglhraglaq ageapgtlag
       61 alppgqrhsa asltalaaqg vrggkrddye eqydddddei mgmsgvarlk kgeqmdklqp
      121 flrdieklys rgfrrshvmr skaahdgwkd liriqhnedq qllaadaqdr natvggealp
      181 nvflagrkqv nisalpyllk qptltvqard lshddkvsfy msvsfedqva fhwylngrpl
      241 eeydsmqild mntvidaank sthsrvsqie iehllklpts tgkyeincta tvdytqhslk
      301 yviepqltdd cppancydrh aicrngkcvc dspypvrlss vhttcrtesf letpchhheq
      361 cqyttnnsec leqgwcackr sfgrtpqhvc vkktgvnarc dtdgqcalyn atcilshctc
      421 nsdsmeendh cvvvsrsvnh lhsattavkp rsataalava valggfcgll vs
//
LOCUS       XP_077502638             318 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113438 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502638
VERSION     XP_077502638.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646512.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..318
                     /product="uncharacterized protein LOC144113438 isoform X1"
                     /calculated_mol_wt=36762
     Region          103..208
                     /region_name="PFM_aerolysin_family"
                     /note="pore-forming module of aerolysin-type beta-barrel
                     pore-forming proteins; cl40431"
                     /db_xref="CDD:394801"
     Region          125..189
                     /region_name="putative membrane-spanning region"
                     /note="putative membrane-spanning region [structural
                     motif]"
                     /db_xref="CDD:380782"
     CDS             1..318
                     /gene="LOC144113438"
                     /coded_by="XM_077646512.1:207..1163"
                     /db_xref="GeneID:144113438"
ORIGIN      
        1 mdmasllnsv lgicgvvlvl ivlqntavda kkvfdvnskv kdfirklerq hktkvswwdm
       61 ygkqrwlvdr gdqykkyqvt akadpmtlda esnrtlesdr tvksytvyvn rfenhqpdhe
      121 qktlirrttr rekevswfst ksinidvdvr atlslfgvfn lggsfeysyd lrsgerhvek
      181 eveefsieke itipprstve vewvitetve scpwksdvvl dgwfaiwfer pvqrhhlwfh
      241 pidavrdpdl klhdpsgvvy ttegfavgvt tvrndlrvrq yshegpqnet iyavplnesd
      301 vvsttlirtp lddaedeh
//
LOCUS       XP_077502639             318 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113438 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502639
VERSION     XP_077502639.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646513.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..318
                     /product="uncharacterized protein LOC144113438 isoform X2"
                     /calculated_mol_wt=37073
     Region          103..208
                     /region_name="PFM_aerolysin_family"
                     /note="pore-forming module of aerolysin-type beta-barrel
                     pore-forming proteins; cl40431"
                     /db_xref="CDD:394801"
     Region          125..189
                     /region_name="putative membrane-spanning region"
                     /note="putative membrane-spanning region [structural
                     motif]"
                     /db_xref="CDD:380782"
     CDS             1..318
                     /gene="LOC144113438"
                     /coded_by="XM_077646513.1:16..972"
                     /db_xref="GeneID:144113438"
ORIGIN      
        1 mtatqhepvy fttcpqfadk rvlqntavda kkvfdvnskv kdfirklerq hktkvswwdm
       61 ygkqrwlvdr gdqykkyqvt akadpmtlda esnrtlesdr tvksytvyvn rfenhqpdhe
      121 qktlirrttr rekevswfst ksinidvdvr atlslfgvfn lggsfeysyd lrsgerhvek
      181 eveefsieke itipprstve vewvitetve scpwksdvvl dgwfaiwfer pvqrhhlwfh
      241 pidavrdpdl klhdpsgvvy ttegfavgvt tvrndlrvrq yshegpqnet iyavplnesd
      301 vvsttlirtp lddaedeh
//
LOCUS       XP_077502640             994 aa            linear   INV 25-NOV-2025
DEFINITION  alanine--tRNA ligase, cytoplasmic-like [Amblyomma americanum].
ACCESSION   XP_077502640
VERSION     XP_077502640.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646514.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..994
                     /product="alanine--tRNA ligase, cytoplasmic-like"
                     /calculated_mol_wt=110577
     Region          28..937
                     /region_name="PLN02900"
                     /note="alanyl-tRNA synthetase"
                     /db_xref="CDD:215487"
     CDS             1..994
                     /gene="LOC144113439"
                     /coded_by="XM_077646514.1:131..3115"
                     /db_xref="GeneID:144113439"
ORIGIN      
        1 mtgkaltacg tqltwlrfkn mrgfctrptk klsarearqa fvdyfvrdha hqfvpsssvv
       61 pavadrsvlf tsagmnqfkg vflgtvepsn qfynlqraan yqkcirvggk hndlddvgkd
      121 lthhtffeml gswsfgdyfk keacamawel ltkvyqlpke rlyvtyfagd palglqpdee
      181 crqvwldlgv pethvlpfgm kenfwemgdv gpcgpcseih idlaesnsas saavhrvnag
      241 rsdmvelwnl vfiefdrdan ahlhalpkkh vdtgmglerl aailqnvksn ydtdlflpli
      301 ealqkkarvn pysglvgsnc svdiayrtav dharmftmai sdgvlpehad agnklrriir
      361 kashaviwhl kcdrgtlahl vdevnfllgd iypginvnlv knvvnseedk ylwqmskaea
      421 alneakpanv isghtawemy sqygiqkdli adlaaergls idwelfsqlf kefqeksasg
      481 qarhaehlvh elpghaehlr dtgilptdtq giysyvsrng dyefpslkat vkaiffngqn
      541 vasvrhgeqc livtdatnfy fesggqvsdi gciktkngev tvqnvkdcgg fvfheglvta
      601 glvhrddvae mcidkqhrtr cmmhhtathc lnaalrmvlg steqrsslvd sdhlrfdfma
      661 eknltneqia evqdicremi qdgctvtrtv mlqadalalp klvkvpnety pstvtvisig
      721 nggdlfsqel ccgthvssls dlgdivvtsh qsmgtmvrsv cavagplava vrsqdesiqr
      781 qmhelaeeid tlahipldny irmaackrkl tsirkiaaar eislllqrks esellkmerc
      841 idsiirkynr sfgearvlah lnealeiwes ssfvvscldf cadgnlvskl afkkckpkpy
      901 fvavythpde lridcavpqe yltstfraea wvnavgpyvm lkkfmssksh ttetycnvka
      961 sfgdlnikav eeaavsfaka hcgqvskkqs nvhl
//
LOCUS       XP_077502641             994 aa            linear   INV 25-NOV-2025
DEFINITION  alanine--tRNA ligase, cytoplasmic-like [Amblyomma americanum].
ACCESSION   XP_077502641
VERSION     XP_077502641.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646515.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..994
                     /product="alanine--tRNA ligase, cytoplasmic-like"
                     /calculated_mol_wt=110577
     Region          28..937
                     /region_name="PLN02900"
                     /note="alanyl-tRNA synthetase"
                     /db_xref="CDD:215487"
     CDS             1..994
                     /gene="LOC144113439"
                     /coded_by="XM_077646515.1:135..3119"
                     /db_xref="GeneID:144113439"
ORIGIN      
        1 mtgkaltacg tqltwlrfkn mrgfctrptk klsarearqa fvdyfvrdha hqfvpsssvv
       61 pavadrsvlf tsagmnqfkg vflgtvepsn qfynlqraan yqkcirvggk hndlddvgkd
      121 lthhtffeml gswsfgdyfk keacamawel ltkvyqlpke rlyvtyfagd palglqpdee
      181 crqvwldlgv pethvlpfgm kenfwemgdv gpcgpcseih idlaesnsas saavhrvnag
      241 rsdmvelwnl vfiefdrdan ahlhalpkkh vdtgmglerl aailqnvksn ydtdlflpli
      301 ealqkkarvn pysglvgsnc svdiayrtav dharmftmai sdgvlpehad agnklrriir
      361 kashaviwhl kcdrgtlahl vdevnfllgd iypginvnlv knvvnseedk ylwqmskaea
      421 alneakpanv isghtawemy sqygiqkdli adlaaergls idwelfsqlf kefqeksasg
      481 qarhaehlvh elpghaehlr dtgilptdtq giysyvsrng dyefpslkat vkaiffngqn
      541 vasvrhgeqc livtdatnfy fesggqvsdi gciktkngev tvqnvkdcgg fvfheglvta
      601 glvhrddvae mcidkqhrtr cmmhhtathc lnaalrmvlg steqrsslvd sdhlrfdfma
      661 eknltneqia evqdicremi qdgctvtrtv mlqadalalp klvkvpnety pstvtvisig
      721 nggdlfsqel ccgthvssls dlgdivvtsh qsmgtmvrsv cavagplava vrsqdesiqr
      781 qmhelaeeid tlahipldny irmaackrkl tsirkiaaar eislllqrks esellkmerc
      841 idsiirkynr sfgearvlah lnealeiwes ssfvvscldf cadgnlvskl afkkckpkpy
      901 fvavythpde lridcavpqe yltstfraea wvnavgpyvm lkkfmssksh ttetycnvka
      961 sfgdlnikav eeaavsfaka hcgqvskkqs nvhl
//
LOCUS       XP_077502642             582 aa            linear   INV 25-NOV-2025
DEFINITION  LIM domain-binding protein 2-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502642
VERSION     XP_077502642.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646516.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..582
                     /product="LIM domain-binding protein 2-like isoform X1"
                     /calculated_mol_wt=62542
     Region          182..411
                     /region_name="LIM_bind"
                     /note="LIM-domain binding protein; pfam01803"
                     /db_xref="CDD:426444"
     Region          490..518
                     /region_name="LID"
                     /note="LIM interaction domain (LID); pfam17916"
                     /db_xref="CDD:465564"
     CDS             1..582
                     /gene="LOC144113440"
                     /coded_by="XM_077646516.1:766..2514"
                     /db_xref="GeneID:144113440"
ORIGIN      
        1 mvvfikeeqk pgpaqgahel atgtvsprhc vrnsptgsgg gpglvaqapa hgpahgpghg
       61 ppgpgpaahg pphgpahspa hgpppphgpp appphgpppp hgpgmepdgs wkgpapphhh
      121 palmghgpam hhgppppphh gppppphpgp phpmagppht mpdgrplehg rhtpyfgqpd
      181 yriyemnkrl qqrteesdnl wwdafateff eddatltltf fledgpkryt igrtlipryf
      241 rsifeggvtd lyfnlkhpke sfhnttitld cdqctmvthh gkppyskglg gqypadplke
      301 qlakdsaviv ctegrlilef tfddlmriks whfatrthre lvprsliaiq vryaaqqqdp
      361 gmleqlskni trqgltnstl nylrlcvile pmqelmsrhk ayalsprdcl kttlfqkwqr
      421 mvappgvahr gapndlksaq dietqrppsk rrkrkssaan naggasnags ggkkknmspg
      481 ppnfslasqd vmvvgepslm ggefgdeder vitrlentqy dasaaaangl eegdefgggp
      541 lapppgwqgp ppgpppernp sapppgpppp pedkktspsi sq
//
LOCUS       XP_077502643             578 aa            linear   INV 25-NOV-2025
DEFINITION  LIM domain-binding protein 2-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502643
VERSION     XP_077502643.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646517.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..578
                     /product="LIM domain-binding protein 2-like isoform X2"
                     /calculated_mol_wt=62052
     Region          182..407
                     /region_name="LIM_bind"
                     /note="LIM-domain binding protein; pfam01803"
                     /db_xref="CDD:426444"
     Region          486..514
                     /region_name="LID"
                     /note="LIM interaction domain (LID); pfam17916"
                     /db_xref="CDD:465564"
     CDS             1..578
                     /gene="LOC144113440"
                     /coded_by="XM_077646517.1:766..2502"
                     /db_xref="GeneID:144113440"
ORIGIN      
        1 mvvfikeeqk pgpaqgahel atgtvsprhc vrnsptgsgg gpglvaqapa hgpahgpghg
       61 ppgpgpaahg pphgpahspa hgpppphgpp appphgpppp hgpgmepdgs wkgpapphhh
      121 palmghgpam hhgppppphh gppppphpgp phpmagppht mpdgrplehg rhtpyfgqpd
      181 yriyemnkrl qqrteesdnl wwdafateff eddatltltf fledgpkryt igrtlipryf
      241 rsifeggvtd lyfnlkhpke sfhnttitld cdqctmvthh gkppyskglg gqypadplke
      301 qlakdsaviv ctegrlilef tfddlmriks whfatrthre lvprsliaiq aqqqdpgmle
      361 qlsknitrqg ltnstlnylr lcvilepmqe lmsrhkayal sprdclkttl fqkwqrmvap
      421 pgvahrgapn dlksaqdiet qrppskrrkr kssaannagg asnagsggkk knmspgppnf
      481 slasqdvmvv gepslmggef gdedervitr lentqydasa aaangleegd efgggplapp
      541 pgwqgpppgp ppernpsapp pgpppppedk ktspsisq
//
LOCUS       XP_077502644             569 aa            linear   INV 25-NOV-2025
DEFINITION  LIM domain-binding protein 2-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502644
VERSION     XP_077502644.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646518.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..569
                     /product="LIM domain-binding protein 2-like isoform X3"
                     /calculated_mol_wt=61247
     Region          169..398
                     /region_name="LIM_bind"
                     /note="LIM-domain binding protein; pfam01803"
                     /db_xref="CDD:426444"
     Region          477..505
                     /region_name="LID"
                     /note="LIM interaction domain (LID); pfam17916"
                     /db_xref="CDD:465564"
     CDS             1..569
                     /gene="LOC144113440"
                     /coded_by="XM_077646518.1:330..2039"
                     /db_xref="GeneID:144113440"
ORIGIN      
        1 mrlrpelatg tvsprhcvrn sptgsgggpg lvaqapahgp ahgpghgppg pgpaahgpph
       61 gpahspahgp ppphgppapp phgpppphgp gmepdgswkg papphhhpal mghgpamhhg
      121 ppppphhgpp ppphpgpphp magpphtmpd grplehgrht pyfgqpdyri yemnkrlqqr
      181 teesdnlwwd afateffedd atltltffle dgpkrytigr tlipryfrsi feggvtdlyf
      241 nlkhpkesfh nttitldcdq ctmvthhgkp pyskglggqy padplkeqla kdsavivcte
      301 grlileftfd dlmrikswhf atrthrelvp rsliaiqvry aaqqqdpgml eqlsknitrq
      361 gltnstlnyl rlcvilepmq elmsrhkaya lsprdclktt lfqkwqrmva ppgvahrgap
      421 ndlksaqdie tqrppskrrk rkssaannag gasnagsggk kknmspgppn fslasqdvmv
      481 vgepslmgge fgdedervit rlentqydas aaaangleeg defgggplap ppgwqgpppg
      541 pppernpsap ppgppppped kktspsisq
//
LOCUS       XP_077502645             569 aa            linear   INV 25-NOV-2025
DEFINITION  LIM domain-binding protein 2-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502645
VERSION     XP_077502645.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646519.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..569
                     /product="LIM domain-binding protein 2-like isoform X3"
                     /calculated_mol_wt=61247
     Region          169..398
                     /region_name="LIM_bind"
                     /note="LIM-domain binding protein; pfam01803"
                     /db_xref="CDD:426444"
     Region          477..505
                     /region_name="LID"
                     /note="LIM interaction domain (LID); pfam17916"
                     /db_xref="CDD:465564"
     CDS             1..569
                     /gene="LOC144113440"
                     /coded_by="XM_077646519.1:115..1824"
                     /db_xref="GeneID:144113440"
ORIGIN      
        1 mrlrpelatg tvsprhcvrn sptgsgggpg lvaqapahgp ahgpghgppg pgpaahgpph
       61 gpahspahgp ppphgppapp phgpppphgp gmepdgswkg papphhhpal mghgpamhhg
      121 ppppphhgpp ppphpgpphp magpphtmpd grplehgrht pyfgqpdyri yemnkrlqqr
      181 teesdnlwwd afateffedd atltltffle dgpkrytigr tlipryfrsi feggvtdlyf
      241 nlkhpkesfh nttitldcdq ctmvthhgkp pyskglggqy padplkeqla kdsavivcte
      301 grlileftfd dlmrikswhf atrthrelvp rsliaiqvry aaqqqdpgml eqlsknitrq
      361 gltnstlnyl rlcvilepmq elmsrhkaya lsprdclktt lfqkwqrmva ppgvahrgap
      421 ndlksaqdie tqrppskrrk rkssaannag gasnagsggk kknmspgppn fslasqdvmv
      481 vgepslmgge fgdedervit rlentqydas aaaangleeg defgggplap ppgwqgpppg
      541 pppernpsap ppgppppped kktspsisq
//
LOCUS       XP_077502646             565 aa            linear   INV 25-NOV-2025
DEFINITION  LIM domain-binding protein 2-like isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077502646
VERSION     XP_077502646.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646520.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..565
                     /product="LIM domain-binding protein 2-like isoform X4"
                     /calculated_mol_wt=60811
     Region          182..411
                     /region_name="LIM_bind"
                     /note="LIM-domain binding protein; pfam01803"
                     /db_xref="CDD:426444"
     Region          473..501
                     /region_name="LID"
                     /note="LIM interaction domain (LID); pfam17916"
                     /db_xref="CDD:465564"
     CDS             1..565
                     /gene="LOC144113440"
                     /coded_by="XM_077646520.1:769..2466"
                     /db_xref="GeneID:144113440"
ORIGIN      
        1 mvvfikeeqk pgpaqgahel atgtvsprhc vrnsptgsgg gpglvaqapa hgpahgpghg
       61 ppgpgpaahg pphgpahspa hgpppphgpp appphgpppp hgpgmepdgs wkgpapphhh
      121 palmghgpam hhgppppphh gppppphpgp phpmagppht mpdgrplehg rhtpyfgqpd
      181 yriyemnkrl qqrteesdnl wwdafateff eddatltltf fledgpkryt igrtlipryf
      241 rsifeggvtd lyfnlkhpke sfhnttitld cdqctmvthh gkppyskglg gqypadplke
      301 qlakdsaviv ctegrlilef tfddlmriks whfatrthre lvprsliaiq vryaaqqqdp
      361 gmleqlskni trqgltnstl nylrlcvile pmqelmsrhk ayalsprdcl kttlfqkwqr
      421 mvappetqrp pskrrkrkss aannaggasn agsggkkknm spgppnfsla sqdvmvvgep
      481 slmggefgde dervitrlen tqydasaaaa ngleegdefg ggplapppgw qgpppgpppe
      541 rnpsapppgp ppppedkkts psisq
//
LOCUS       XP_077502647             561 aa            linear   INV 25-NOV-2025
DEFINITION  LIM domain-binding protein 2-like isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077502647
VERSION     XP_077502647.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646521.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..561
                     /product="LIM domain-binding protein 2-like isoform X5"
                     /calculated_mol_wt=60321
     Region          182..407
                     /region_name="LIM_bind"
                     /note="LIM-domain binding protein; pfam01803"
                     /db_xref="CDD:426444"
     Region          469..497
                     /region_name="LID"
                     /note="LIM interaction domain (LID); pfam17916"
                     /db_xref="CDD:465564"
     CDS             1..561
                     /gene="LOC144113440"
                     /coded_by="XM_077646521.1:769..2454"
                     /db_xref="GeneID:144113440"
ORIGIN      
        1 mvvfikeeqk pgpaqgahel atgtvsprhc vrnsptgsgg gpglvaqapa hgpahgpghg
       61 ppgpgpaahg pphgpahspa hgpppphgpp appphgpppp hgpgmepdgs wkgpapphhh
      121 palmghgpam hhgppppphh gppppphpgp phpmagppht mpdgrplehg rhtpyfgqpd
      181 yriyemnkrl qqrteesdnl wwdafateff eddatltltf fledgpkryt igrtlipryf
      241 rsifeggvtd lyfnlkhpke sfhnttitld cdqctmvthh gkppyskglg gqypadplke
      301 qlakdsaviv ctegrlilef tfddlmriks whfatrthre lvprsliaiq aqqqdpgmle
      361 qlsknitrqg ltnstlnylr lcvilepmqe lmsrhkayal sprdclkttl fqkwqrmvap
      421 petqrppskr rkrkssaann aggasnagsg gkkknmspgp pnfslasqdv mvvgepslmg
      481 gefgdederv itrlentqyd asaaaangle egdefgggpl apppgwqgpp pgpppernps
      541 apppgppppp edkktspsis q
//
LOCUS       XP_077502648             376 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113443 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502648
VERSION     XP_077502648.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646522.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..376
                     /product="uncharacterized protein LOC144113443 isoform X1"
                     /calculated_mol_wt=42743
     CDS             1..376
                     /gene="LOC144113443"
                     /coded_by="XM_077646522.1:103..1233"
                     /db_xref="GeneID:144113443"
ORIGIN      
        1 mfayvrfrdd dmktvlpisr frsfqpkglk dfatnkwyev fwedneqsgy yqaqivrlfd
       61 tkeeaerqkr ipvpprpsdt etelttpssd dslrevmrka aaekkkkknn sqrilrekql
      121 qqlmenepde qaheleqlrr enkdlrerlk tmekalcski fdaeqlmsrh csckqkrpei
      181 rtservpakg avermpalsa pagpekvppa apeakpadpp aresqrpvav lappqpslgh
      241 npatpeecaa dqleglpfqa tgskvflghg vsltregfnh lmsrqkdsvf vreacvqifs
      301 taglvgksit gnmcnrtkad pkppldqqky aalsaffqhy learyeadea rrrhrslnki
      361 vagkladvmk mqrrdv
//
LOCUS       XP_077502649             350 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113443 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502649
VERSION     XP_077502649.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646523.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..350
                     /product="uncharacterized protein LOC144113443 isoform X2"
                     /calculated_mol_wt=39789
     CDS             1..350
                     /gene="LOC144113443"
                     /coded_by="XM_077646523.1:107..1159"
                     /db_xref="GeneID:144113443"
ORIGIN      
        1 mfayvrfrdd dmktvlpisr frsfqpkglk dfatnkwyev fwedneqsgy yqaqivrlfd
       61 tkeeaerqkr ipvpprpsdt etelttpssd dslrevmrka aaekkkkknn sqrilrekql
      121 qqlmenepde qaheleqlrr enkdlrerlk tmekalcski fdaeqlmsrh csckqkrpei
      181 rtservpakg avermpalsa pagpekvppa apeakpadpp aresqrpvav lappqpslgh
      241 npatpeecaa dqleglpfqa tgskvflghg vsltreglvg ksitgnmcnr tkadpkppld
      301 qqkyaalsaf fqhylearye adearrrhrs lnkivagkla dvmkmqrrdv
//
LOCUS       XP_077502651             822 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113444 [Amblyomma americanum].
ACCESSION   XP_077502651
VERSION     XP_077502651.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646525.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..822
                     /product="uncharacterized protein LOC144113444"
                     /calculated_mol_wt=92241
     CDS             1..822
                     /gene="LOC144113444"
                     /coded_by="XM_077646525.1:86..2554"
                     /db_xref="GeneID:144113444"
ORIGIN      
        1 mplsvifesn ktaakitkst sttiatenrm kraftrtvvl rtkttctavl rakttsamia
       61 aemrtkktli vatlrtktms ltiatetrtk ttrtvavvrt tatviessvi ffsierlpns
      121 dvtvrdamfi lmtytsssgl nwvdmeklvg vinlflgkpi lptsqyllrk vwkrweadmv
      181 krhyfceecg aiipnpekre flcpscntsn saqnffatvs iekqlevmlt ndtvaqtllt
      241 slkkkratas dpgpsenkvi rditdgklyq rqmgkaawsd vsltfntdga rafkcskssl
      301 wpiqavvnel pvplrwkhvl lcglyfgksh pnmasflevf tetvrrtvnw ecdgekyssn
      361 mkftcccvda paraavlnik qfngyygcsy chhkgvlvnr svkypvaddg ghpeprtnrs
      421 vrsdmkslis gepsrgvkgf splarlpdfd lvwgvcpdym hcvlegvtrq ladtwfgcvq
      481 sasyigvpat lsqvntrlls ikplqwfsrl pcsigeralw kasewkwwll fysvpclegi
      541 lpeeylhhfc slvaavyill kdemtkedih eamdklscfv lsmeelygre amtfnvhqll
      601 hlpksvlelg plwshsafvf esgngmllkl isdangvplq ilerfamrlq itklantlnp
      661 tsqvqavydt lmpsscagks stekplgrgt llnemdeqvr cavqeklgqv lplmkfkrmd
      721 vhgqrlhveg yrrakktrnc ifrcvqgkyy ilesvfqlpd stclllctcl asesyrdlph
      781 ihkcaktskk vvlaadkvss vsvamdvgrv cyvgdmpnlf ed
//
LOCUS       XP_077502652             243 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113445 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502652
VERSION     XP_077502652.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646526.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..243
                     /product="uncharacterized protein LOC144113445 isoform X1"
                     /calculated_mol_wt=26772
     CDS             1..243
                     /gene="LOC144113445"
                     /coded_by="XM_077646526.1:143..874"
                     /db_xref="GeneID:144113445"
ORIGIN      
        1 msrgrpfqsa allrkedaar ifcrniweql grngiygeic wlgeqdmvhn ealalflqvq
       61 pslgcaeegf taalpyqpdi ksfarasrcp vieivqpffv stsatpsvts shvdipaair
      121 scppaistsg lgsstsdmml nqfcfivqma lvlwcslvqh nsdclcgrsr lgiwrsarsc
      181 lvqqricgvp rksriyqnda atgaftacgc dvqvvclrwl gtilyhisii lfqqippfrv
      241 hqt
//
LOCUS       XP_077502653             210 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113445 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502653
VERSION     XP_077502653.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646527.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..210
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..210
                     /product="uncharacterized protein LOC144113445 isoform X2"
                     /calculated_mol_wt=22900
     CDS             1..210
                     /gene="LOC144113445"
                     /coded_by="XM_077646527.1:207..839"
                     /db_xref="GeneID:144113445"
ORIGIN      
        1 mpkplqtscl qrhlpqisst gadvtvqlvs fisvfkrrav msrgrpfqsa allrkedaar
       61 ifcrniweql grngiygeic wlgeqdmvhn ealalflqvq pslgcaeegf taalpyqpdi
      121 ksfarasrcp vieivqpffv stsatpsvts shvdipaair scppaistsg lgsstsdmml
      181 nqfcfivqma lvlwcslvqh nsdclcemtg
//
LOCUS       XP_077502654             226 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113446 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502654
VERSION     XP_077502654.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646528.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..226
                     /product="uncharacterized protein LOC144113446 isoform X1"
                     /calculated_mol_wt=24710
     CDS             1..226
                     /gene="LOC144113446"
                     /coded_by="XM_077646528.1:261..941"
                     /db_xref="GeneID:144113446"
ORIGIN      
        1 mpkplqtsfl qrhlpqisst gadvtvqlvs fisvfkrrav msrgrpfqsa allrkedaar
       61 ifcrniweql grngiygeic wlgeqdmvhn ealalflqvq pslgcaeegf taalpyqpdi
      121 ksfarasrcp vieivqpffv ptsatpsvts shvdipaair scppaistsg lgsstsdmml
      181 nqfcfivqma lvlwcslvqh nsdclcepsq nrgarpnygm ssrlta
//
LOCUS       XP_077502655             186 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113446 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502655
VERSION     XP_077502655.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646529.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..186
                     /product="uncharacterized protein LOC144113446 isoform X2"
                     /calculated_mol_wt=20243
     CDS             1..186
                     /gene="LOC144113446"
                     /coded_by="XM_077646529.1:523..1083"
                     /db_xref="GeneID:144113446"
ORIGIN      
        1 msrgrpfqsa allrkedaar ifcrniweql grngiygeic wlgeqdmvhn ealalflqvq
       61 pslgcaeegf taalpyqpdi ksfarasrcp vieivqpffv ptsatpsvts shvdipaair
      121 scppaistsg lgsstsdmml nqfcfivqma lvlwcslvqh nsdclcepsq nrgarpnygm
      181 ssrlta
//
LOCUS       XP_077502656             120 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113446 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502656
VERSION     XP_077502656.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646530.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..120
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..120
                     /product="uncharacterized protein LOC144113446 isoform X3"
                     /calculated_mol_wt=12932
     CDS             1..120
                     /gene="LOC144113446"
                     /coded_by="XM_077646530.1:1135..1497"
                     /db_xref="GeneID:144113446"
ORIGIN      
        1 mvpggsavgp trsaaclyst reagrrqvcq gtqpekrcqa evwavfspta efpeqssasp
       61 evqgsgcths gsrppsrllr rgkmaashtp craprreqsl drlrndnvql rgqvlsekel
//
LOCUS       XP_077502657             119 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113446 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077502657
VERSION     XP_077502657.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646531.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..119
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..119
                     /product="uncharacterized protein LOC144113446 isoform X4"
                     /calculated_mol_wt=12801
     CDS             1..119
                     /gene="LOC144113446"
                     /coded_by="XM_077646531.1:1135..1494"
                     /db_xref="GeneID:144113446"
ORIGIN      
        1 mvpggsavgp trsaaclyst reagrrqvcq gtqpekrcqa evwavfspta efpeqssasp
       61 evqgsgcths gsrppsrllr rgkmaashtp craprreqsl drlrndnvql rgqlgadrr
//
LOCUS       XP_077502658             104 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113447 [Amblyomma americanum].
ACCESSION   XP_077502658
VERSION     XP_077502658.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646532.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..104
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..104
                     /product="uncharacterized protein LOC144113447"
                     /calculated_mol_wt=10993
     CDS             1..104
                     /gene="LOC144113447"
                     /coded_by="XM_077646532.1:242..556"
                     /db_xref="GeneID:144113447"
ORIGIN      
        1 mltstagtvp seragglgwn parkevpsrs mgcllancsi ssaspevqgs gcthsgsrpp
       61 srllrrgkma ashtpcrapr reqsldrlrn dnvqlrgqlg adrr
//
LOCUS       XP_077502659             717 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 1-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502659
VERSION     XP_077502659.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646533.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..717
                     /product="endothelin-converting enzyme 1-like isoform X1"
                     /calculated_mol_wt=81955
     Region          99..717
                     /region_name="M13"
                     /note="Peptidase family M13 includes neprilysin and
                     endothelin-converting enzyme I; cd08662"
                     /db_xref="CDD:341056"
     Site            order(519,524..526,529,547,563..564,567..568,571,624,669,
                     672..673,685..686)
                     /site_type="active"
                     /db_xref="CDD:341056"
     CDS             1..717
                     /gene="LOC144113450"
                     /coded_by="XM_077646533.1:291..2444"
                     /db_xref="GeneID:144113450"
ORIGIN      
        1 maqllvsels pdissylilv scfppldyvs rdtmakehaf ysralnycif ttvvfavcfa
       61 anypivrwif girtkhcitp acerftrdvr tsmnrrqppc edfyqftcgr wqhthpgfpn
      121 qtefatsklv aeivknlkrv enipdknqta lqkaaavfqs clsmlngeet nsealvsfie
      181 nlgitwpagg tksqlkalnv lvklslsvdv hllfvvdayv dysetyslri svpnhmlyaq
      241 shnytskerk kevlsvlygn nsfdamltvl akfeekiqqr mlsvnavrrf tdtialrgig
      301 tympnqpase wvqalnkelp gdkavtenta vrvddvahle aiselvyele dqmllswfvg
      361 wnvlrefale asyqlgtala gttetfnekh cfyavrdnlh faltarslls vaepstrtai
      421 sstasavwrs ymhsirandw mdmetktral ekavqmvlld gyprfvddpe nlnifysyip
      481 dltkdfmttt illrearmny mksyihvpay ktnlekwnyp llsldslrfh nnlnklvvpv
      541 aaateplylk yfptswkygg lgtaiaekmy hafdvtgkdf dsfggpsdww snntrlknnk
      601 rslcylkdfv qwsegapgls tveqyralla siegvrqafr ayalktngnk krlrtidfla
      661 psqifflsyc frwcskdepr namnvhmcnf playskqftd vygcqkgdrm yfrrkcs
//
LOCUS       XP_077502660             684 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 1-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502660
VERSION     XP_077502660.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646534.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..684
                     /product="endothelin-converting enzyme 1-like isoform X2"
                     /calculated_mol_wt=78300
     Region          66..684
                     /region_name="M13"
                     /note="Peptidase family M13 includes neprilysin and
                     endothelin-converting enzyme I; cd08662"
                     /db_xref="CDD:341056"
     Site            order(486,491..493,496,514,530..531,534..535,538,591,636,
                     639..640,652..653)
                     /site_type="active"
                     /db_xref="CDD:341056"
     CDS             1..684
                     /gene="LOC144113450"
                     /coded_by="XM_077646534.1:505..2559"
                     /db_xref="GeneID:144113450"
ORIGIN      
        1 makehafysr alnycifttv vfavcfaany pivrwifgir tkhcitpace rftrdvrtsm
       61 nrrqppcedf yqftcgrwqh thpgfpnqte fatsklvaei vknlkrveni pdknqtalqk
      121 aaavfqscls mlngeetnse alvsfienlg itwpaggtks qlkalnvlvk lslsvdvhll
      181 fvvdayvdys etyslrisvp nhmlyaqshn ytskerkkev lsvlygnnsf damltvlakf
      241 eekiqqrmls vnavrrftdt ialrgigtym pnqpasewvq alnkelpgdk avtentavrv
      301 ddvahleais elvyeledqm llswfvgwnv lrefaleasy qlgtalagtt etfnekhcfy
      361 avrdnlhfal tarsllsvae pstrtaisst asavwrsymh sirandwmdm etktraleka
      421 vqmvlldgyp rfvddpenln ifysyipdlt kdfmtttill rearmnymks yihvpayktn
      481 lekwnyplls ldslrfhnnl nklvvpvaaa teplylkyfp tswkygglgt aiaekmyhaf
      541 dvtgkdfdsf ggpsdwwsnn trlknnkrsl cylkdfvqws egapglstve qyrallasie
      601 gvrqafraya lktngnkkrl rtidflapsq ifflsycfrw cskdeprnam nvhmcnfpla
      661 yskqftdvyg cqkgdrmyfr rkcs
//
LOCUS       XP_077502662             684 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 1-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502662
VERSION     XP_077502662.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646536.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..684
                     /product="endothelin-converting enzyme 1-like isoform X2"
                     /calculated_mol_wt=78300
     Region          66..684
                     /region_name="M13"
                     /note="Peptidase family M13 includes neprilysin and
                     endothelin-converting enzyme I; cd08662"
                     /db_xref="CDD:341056"
     Site            order(486,491..493,496,514,530..531,534..535,538,591,636,
                     639..640,652..653)
                     /site_type="active"
                     /db_xref="CDD:341056"
     CDS             1..684
                     /gene="LOC144113450"
                     /coded_by="XM_077646536.1:310..2364"
                     /db_xref="GeneID:144113450"
ORIGIN      
        1 makehafysr alnycifttv vfavcfaany pivrwifgir tkhcitpace rftrdvrtsm
       61 nrrqppcedf yqftcgrwqh thpgfpnqte fatsklvaei vknlkrveni pdknqtalqk
      121 aaavfqscls mlngeetnse alvsfienlg itwpaggtks qlkalnvlvk lslsvdvhll
      181 fvvdayvdys etyslrisvp nhmlyaqshn ytskerkkev lsvlygnnsf damltvlakf
      241 eekiqqrmls vnavrrftdt ialrgigtym pnqpasewvq alnkelpgdk avtentavrv
      301 ddvahleais elvyeledqm llswfvgwnv lrefaleasy qlgtalagtt etfnekhcfy
      361 avrdnlhfal tarsllsvae pstrtaisst asavwrsymh sirandwmdm etktraleka
      421 vqmvlldgyp rfvddpenln ifysyipdlt kdfmtttill rearmnymks yihvpayktn
      481 lekwnyplls ldslrfhnnl nklvvpvaaa teplylkyfp tswkygglgt aiaekmyhaf
      541 dvtgkdfdsf ggpsdwwsnn trlknnkrsl cylkdfvqws egapglstve qyrallasie
      601 gvrqafraya lktngnkkrl rtidflapsq ifflsycfrw cskdeprnam nvhmcnfpla
      661 yskqftdvyg cqkgdrmyfr rkcs
//
LOCUS       XP_077502663             554 aa            linear   INV 25-NOV-2025
DEFINITION  major facilitator superfamily domain-containing protein 10-like
            isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502663
VERSION     XP_077502663.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646537.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..554
                     /product="major facilitator superfamily domain-containing
                     protein 10-like isoform X1"
                     /calculated_mol_wt=59885
     Region          50..446
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(60..61,64..65,68,128,178..179,181..183,186,205,
                     208..209,212,308,311..312,315..317,320,348,352,401..402,
                     406,410,426,429..430,433..434,437)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340947"
     CDS             1..554
                     /gene="LOC144113451"
                     /coded_by="XM_077646537.1:173..1837"
                     /db_xref="GeneID:144113451"
ORIGIN      
        1 mhgtlsypsk fivskpcaaf lvtlcfelal fvqtrmavpt sqgervprtv yvifvylvid
       61 llaftvilpl lpsildyysh ndkdglypwl egcirylqsf igapsslnkv lfggcigsly
      121 sflqfltapl igslsdvygr kwmltlttcg ialsyalwav snnfalfvls riiggvskgn
      181 islstaviad vcnsklrgkg maligiafsv gfivgpliga qfaaaskgst efyvgpalfa
      241 lllalldvvf lclmfteslp qekrahsvas slsgalsyvn paslftfqpv qklplkdhqv
      301 lrqcglayfs yllfysgley slsflthsrf qytrmqqgkm yfmvglvmaa vqggyvhrvp
      361 pgkeirvclv gvmmiipsfi iigvswspyv lysglllyaf ssgtvvpclt tlvskygapt
      421 qkgtilgifr slgalaralg pmlsatgsps pvvdscssss ysgmlgrsnh llvprlrpap
      481 vrvrpqlrpd rsllhdgkhv lcddvartip grnprgvnks cqgifilqts lpvafpates
      541 assfpgiptw lgtq
//
LOCUS       XP_077502664             483 aa            linear   INV 25-NOV-2025
DEFINITION  major facilitator superfamily domain-containing protein 10-like
            isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502664
VERSION     XP_077502664.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646538.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..483
                     /product="major facilitator superfamily domain-containing
                     protein 10-like isoform X2"
                     /calculated_mol_wt=52459
     Region          50..472
                     /region_name="MFS_MFSD10"
                     /note="Major facilitator superfamily domain-containing
                     protein 10; cd17389"
                     /db_xref="CDD:340947"
     Site            order(60..61,64..65,68,128,178..179,181..183,186,205,
                     208..209,212,308,311..312,315..317,320,348,352,401..402,
                     406,410,426,429..430,433..434,437)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340947"
     CDS             1..483
                     /gene="LOC144113451"
                     /coded_by="XM_077646538.1:168..1619"
                     /db_xref="GeneID:144113451"
ORIGIN      
        1 mhgtlsypsk fivskpcaaf lvtlcfelal fvqtrmavpt sqgervprtv yvifvylvid
       61 llaftvilpl lpsildyysh ndkdglypwl egcirylqsf igapsslnkv lfggcigsly
      121 sflqfltapl igslsdvygr kwmltlttcg ialsyalwav snnfalfvls riiggvskgn
      181 islstaviad vcnsklrgkg maligiafsv gfivgpliga qfaaaskgst efyvgpalfa
      241 lllalldvvf lclmfteslp qekrahsvas slsgalsyvn paslftfqpv qklplkdhqv
      301 lrqcglayfs yllfysgley slsflthsrf qytrmqqgkm yfmvglvmaa vqggyvhrvp
      361 pgkeirvclv gvmmiipsfi iigvswspyv lysglllyaf ssgtvvpclt tlvskygapt
      421 qkgtilgifr slgalaralg pmlsatvyws agpvacycig gflfvfplli lrnmhrtvgd
      481 tvk
//
LOCUS       XP_077502665             437 aa            linear   INV 25-NOV-2025
DEFINITION  forkhead box protein F1-like [Amblyomma americanum].
ACCESSION   XP_077502665
VERSION     XP_077502665.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646539.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..437
                     /product="forkhead box protein F1-like"
                     /calculated_mol_wt=46987
     Region          82..159
                     /region_name="FH_FOXF"
                     /note="Forkhead (FH) domain found in the Forkhead box
                     protein F (FOXF) subfamily; cd20020"
                     /db_xref="CDD:410794"
     Site            order(86,104..105,108,127..128,130..131,134,141,151..153,
                     155)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410794"
     CDS             1..437
                     /gene="LOC144113452"
                     /coded_by="XM_077646539.1:231..1544"
                     /db_xref="GeneID:144113452"
ORIGIN      
        1 mkmvkiempm anamdtthda nqlcehtrld elsismpavs ttnncsssnn aslnassepd
       61 sedkvvsttk ksgagvrrqe kppysyiali vmaiqssptk rltlneiynf lqqrfpffrg
      121 tyqgwknsvr hnlslnecfi klpkglgrpg kghywtidps sepmfeegsf rrrprgfrrk
      181 cqtmkaytty yptpsstsll spspydllsq qqhhtgvlpg haqvgtshhq aymspdpqsm
      241 mssstaalap tshcsfpstg qnslgpvghh ylancslpqp mtpmstagsg dlplnssmys
      301 aaapgitmdt msaawsscpp hpsvthssqy ikqpppspvp spvsqmqslp pdlssmsyst
      361 gspslhgqsl lgntadsmdi alagmrfshs gsspcerkvf sagpfsaapl sglgslspga
      421 ptsalpqply yepkyal
//
LOCUS       XP_077502666             271 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113457 [Amblyomma americanum].
ACCESSION   XP_077502666
VERSION     XP_077502666.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646540.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..271
                     /product="uncharacterized protein LOC144113457"
                     /calculated_mol_wt=28765
     Region          29..118
                     /region_name="retropepsin_like"
                     /note="Retropepsins; pepsin-like aspartate proteases;
                     cd00303"
                     /db_xref="CDD:133136"
     Site            order(43,45,47,71..73,111)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133136"
     Site            43..45
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133136"
     Site            43
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133136"
     Site            order(71..74,78..81)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133136"
     CDS             1..271
                     /gene="LOC144113457"
                     /coded_by="XM_077646540.1:319..1134"
                     /db_xref="GeneID:144113457"
ORIGIN      
        1 mqttqisddr almtsttaae lkdrfsldip vlldghsisa lvdtgaefsi isgklaalpe
       61 kdttpcsgvq irmagghiit plgqcmarvq isdstsvvsc lvlldcsrdl ilgvdflhen
      121 gavidlrhrt vtfstaeadd tsdvrqcssa lristesvli pprssvfasi ecddglrdgp
      181 avvegnlsll lthgicaars vvhlrdgrse llitnftsep rhlfrataia wadqladate
      241 cfaseamdrp dtsldridis sglsatsaah l
//
LOCUS       XP_077502667             209 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113458 [Amblyomma americanum].
ACCESSION   XP_077502667
VERSION     XP_077502667.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646541.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..209
                     /product="uncharacterized protein LOC144113458"
                     /calculated_mol_wt=22642
     Region          57..133
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..209
                     /gene="LOC144113458"
                     /coded_by="XM_077646541.1:53..682"
                     /db_xref="GeneID:144113458"
ORIGIN      
        1 mtlgrflsqw hasaakinas gasrrfrvap gcrqlsdtae setsapidnk daampsccvp
       61 gcrsrtpgqe pgvtfhtfpk dperrrqwte avrpgqrdwe pakwtcvcsk hflpehfdrm
      121 splvvrirad avplqapmdv aesqetlagp sqqapmdvte rqetlpgpsq qasmdvatml
      181 diqpgpsqaf qltpelptlp tapasvgtp
//
LOCUS       XP_077502669             206 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113459 [Amblyomma americanum].
ACCESSION   XP_077502669
VERSION     XP_077502669.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646543.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..206
                     /product="uncharacterized protein LOC144113459"
                     /calculated_mol_wt=22217
     CDS             1..206
                     /gene="LOC144113459"
                     /coded_by="XM_077646543.1:216..836"
                     /db_xref="GeneID:144113459"
ORIGIN      
        1 massdvgsds hgilvqvtgv fqepavvggy lflhswtfgk mqahccsphs pvhgrasgia
       61 gaelhrygmc mgnkkkdvqe kgaprplwkf chtklptpac llpnndqest aalicssapn
      121 salafhrkpk qpgqlnsslp vqgiskrktr chscpppllv llkeeakdle sktpegvley
      181 icdvygpetv rkkierstvc tsmaqi
//
LOCUS       XP_077502670             189 aa            linear   INV 25-NOV-2025
DEFINITION  transmembrane protein 208 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502670
VERSION     XP_077502670.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646544.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..189
                     /product="transmembrane protein 208 isoform X1"
                     /calculated_mol_wt=21342
     Region          26..185
                     /region_name="TMEM208_SND2"
                     /note="SRP-independent targeting protein 2/TMEM208;
                     pfam05620"
                     /db_xref="CDD:428546"
     CDS             1..189
                     /gene="LOC144113460"
                     /coded_by="XM_077646544.1:226..795"
                     /db_xref="GeneID:144113460"
ORIGIN      
        1 mavmltsvdi dqflkqtsfk pqkgkqgtkg qkqileenkq tikfysimaa aalaghlvth
       61 lvlwrdsitl symllyafsv lvyggciqtm rymarasyse tgqlldggld lnmvqagmge
      121 hlkdliilta ciqtlslvst yawflwlvap gyafyllwvn vlgpwffqpa ppevddkkqr
      181 klerksrrh
//
LOCUS       XP_077502671             171 aa            linear   INV 25-NOV-2025
DEFINITION  transmembrane protein 208 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502671
VERSION     XP_077502671.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646545.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..171
                     /product="transmembrane protein 208 isoform X2"
                     /calculated_mol_wt=19260
     Region          8..167
                     /region_name="TMEM208_SND2"
                     /note="SRP-independent targeting protein 2/TMEM208;
                     pfam05620"
                     /db_xref="CDD:428546"
     CDS             1..171
                     /gene="LOC144113460"
                     /coded_by="XM_077646545.1:224..739"
                     /db_xref="GeneID:144113460"
ORIGIN      
        1 mapqkgkqgt kgqkqileen kqtikfysim aaaalaghlv thlvlwrdsi tlsymllyaf
       61 svlvyggciq tmrymarasy setgqlldgg ldlnmvqagm gehlkdliil taciqtlslv
      121 styawflwlv apgyafyllw vnvlgpwffq pappevddkk qrklerksrr h
//
LOCUS       XP_077502672             539 aa            linear   INV 25-NOV-2025
DEFINITION  DDB1- and CUL4-associated factor 11 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502672
VERSION     XP_077502672.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646546.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..539
                     /product="DDB1- and CUL4-associated factor 11 isoform X1"
                     /calculated_mol_wt=60642
     Region          <185..517
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          227..268
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          275..311
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          316..358
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          365..400
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          406..439
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          447..487
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          493..517
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..539
                     /gene="LOC144113463"
                     /coded_by="XM_077646546.1:384..2003"
                     /db_xref="GeneID:144113463"
ORIGIN      
        1 msltgsswrm cerppsesas grnsenpgry rvvrtddpny arvlqfllrs ieanrarlfs
       61 salevfyqdd sddelqlngl ytldmgrglm srrcyfrlvs aasspdnsri lkndlytivn
      121 qqsgsknrgk peqgtsmasl lqqrecgmpf rgiftrgdkc rmssrflpnv tagtlfypsk
      181 tfcgtfarng difmtacqdc viriydtshg dfdelkcvsa rdvgwsildm avspdglyfa
      241 ysswseylhl cnvygdhqvh ealalcpedr rfcifslqfs cdgkdilgga ndsclyvydr
      301 eahhrslrir ahdgdvnava fadktsqilf sggddglckv wdrrtlserd pkpvgilagh
      361 cngitfvdsk ndarylitns kdqtiklwdm rafsskegiq atkmavacqn wdyrwqrvpk
      421 klldlceklp gdtslmtyqg htvlqtlirc hfspiattgq qfiytgcasg rvvvynlltg
      481 kietvlsghr acvrdvswhp ylpmlvsssw dgsvtkwvyn sepeskpvlk gklveehne
//
LOCUS       XP_077502673             538 aa            linear   INV 25-NOV-2025
DEFINITION  DDB1- and CUL4-associated factor 11 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502673
VERSION     XP_077502673.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646547.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..538
                     /product="DDB1- and CUL4-associated factor 11 isoform X2"
                     /calculated_mol_wt=60394
     Region          <184..516
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          226..267
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          274..310
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          315..357
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          364..399
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          405..438
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          446..486
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          492..516
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..538
                     /gene="LOC144113463"
                     /coded_by="XM_077646547.1:16..1632"
                     /db_xref="GeneID:144113463"
ORIGIN      
        1 mlcflsllcc lrvditlaaq fypvsvpcra syegyvvsci lnsfvqrcsi eanrarlfss
       61 alevfyqdds ddelqlngly tldmgrglms rrcyfrlvsa asspdnsril kndlytivnq
      121 qsgsknrgkp eqgtsmasll qqrecgmpfr giftrgdkcr mssrflpnvt agtlfypskt
      181 fcgtfarngd ifmtacqdcv iriydtshgd fdelkcvsar dvgwsildma vspdglyfay
      241 sswseylhlc nvygdhqvhe alalcpedrr fcifslqfsc dgkdilggan dsclyvydre
      301 ahhrslrira hdgdvnavaf adktsqilfs ggddglckvw drrtlserdp kpvgilaghc
      361 ngitfvdskn darylitnsk dqtiklwdmr afsskegiqa tkmavacqnw dyrwqrvpkk
      421 lldlceklpg dtslmtyqgh tvlqtlirch fspiattgqq fiytgcasgr vvvynlltgk
      481 ietvlsghra cvrdvswhpy lpmlvssswd gsvtkwvyns epeskpvlkg klveehne
//
LOCUS       XP_077502674             519 aa            linear   INV 25-NOV-2025
DEFINITION  DDB1- and CUL4-associated factor 11 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502674
VERSION     XP_077502674.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646548.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..519
                     /product="DDB1- and CUL4-associated factor 11 isoform X3"
                     /calculated_mol_wt=58251
     Region          <165..497
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          207..248
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          255..291
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          296..338
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          345..380
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          386..419
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          427..467
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          473..497
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..519
                     /gene="LOC144113463"
                     /coded_by="XM_077646548.1:1934..3493"
                     /db_xref="GeneID:144113463"
ORIGIN      
        1 msltgsswrm cerppsesas grnsenpgry rvvrtddpny arvlqfllrs ieanrarlfs
       61 salevfyqdd sddelqlngl sasspdnsri lkndlytivn qqsgsknrgk peqgtsmasl
      121 lqqrecgmpf rgiftrgdkc rmssrflpnv tagtlfypsk tfcgtfarng difmtacqdc
      181 viriydtshg dfdelkcvsa rdvgwsildm avspdglyfa ysswseylhl cnvygdhqvh
      241 ealalcpedr rfcifslqfs cdgkdilgga ndsclyvydr eahhrslrir ahdgdvnava
      301 fadktsqilf sggddglckv wdrrtlserd pkpvgilagh cngitfvdsk ndarylitns
      361 kdqtiklwdm rafsskegiq atkmavacqn wdyrwqrvpk klldlceklp gdtslmtyqg
      421 htvlqtlirc hfspiattgq qfiytgcasg rvvvynlltg kietvlsghr acvrdvswhp
      481 ylpmlvsssw dgsvtkwvyn sepeskpvlk gklveehne
//
LOCUS       XP_077502675             518 aa            linear   INV 25-NOV-2025
DEFINITION  DDB1- and CUL4-associated factor 11 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077502675
VERSION     XP_077502675.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646549.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..518
                     /product="DDB1- and CUL4-associated factor 11 isoform X4"
                     /calculated_mol_wt=58003
     Region          <164..496
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          206..247
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          254..290
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          295..337
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          344..379
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          385..418
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          426..466
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          472..496
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..518
                     /gene="LOC144113463"
                     /coded_by="XM_077646549.1:16..1572"
                     /db_xref="GeneID:144113463"
ORIGIN      
        1 mlcflsllcc lrvditlaaq fypvsvpcra syegyvvsci lnsfvqrcsi eanrarlfss
       61 alevfyqdds ddelqlngls asspdnsril kndlytivnq qsgsknrgkp eqgtsmasll
      121 qqrecgmpfr giftrgdkcr mssrflpnvt agtlfypskt fcgtfarngd ifmtacqdcv
      181 iriydtshgd fdelkcvsar dvgwsildma vspdglyfay sswseylhlc nvygdhqvhe
      241 alalcpedrr fcifslqfsc dgkdilggan dsclyvydre ahhrslrira hdgdvnavaf
      301 adktsqilfs ggddglckvw drrtlserdp kpvgilaghc ngitfvdskn darylitnsk
      361 dqtiklwdmr afsskegiqa tkmavacqnw dyrwqrvpkk lldlceklpg dtslmtyqgh
      421 tvlqtlirch fspiattgqq fiytgcasgr vvvynlltgk ietvlsghra cvrdvswhpy
      481 lpmlvssswd gsvtkwvyns epeskpvlkg klveehne
//
LOCUS       XP_077502676             455 aa            linear   INV 25-NOV-2025
DEFINITION  DDB1- and CUL4-associated factor 11 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077502676
VERSION     XP_077502676.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646550.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..455
                     /product="DDB1- and CUL4-associated factor 11 isoform X5"
                     /calculated_mol_wt=51024
     Region          <101..433
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          143..184
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          191..227
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          232..274
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          281..316
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          322..355
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          363..403
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          409..433
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..455
                     /gene="LOC144113463"
                     /coded_by="XM_077646550.1:304..1671"
                     /db_xref="GeneID:144113463"
ORIGIN      
        1 mgrglmsrrc yfrlvsaass pdnsrilknd lytivnqqsg sknrgkpeqg tsmasllqqr
       61 ecgmpfrgif trgdkcrmss rflpnvtagt lfypsktfcg tfarngdifm tacqdcviri
      121 ydtshgdfde lkcvsardvg wsildmavsp dglyfayssw seylhlcnvy gdhqvheala
      181 lcpedrrfci fslqfscdgk dilggandsc lyvydreahh rslrirahdg dvnavafadk
      241 tsqilfsggd dglckvwdrr tlserdpkpv gilaghcngi tfvdskndar ylitnskdqt
      301 iklwdmrafs skegiqatkm avacqnwdyr wqrvpkklld lceklpgdts lmtyqghtvl
      361 qtlirchfsp iattgqqfiy tgcasgrvvv ynlltgkiet vlsghracvr dvswhpylpm
      421 lvssswdgsv tkwvynsepe skpvlkgklv eehne
//
LOCUS       XP_077502677             455 aa            linear   INV 25-NOV-2025
DEFINITION  DDB1- and CUL4-associated factor 11 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077502677
VERSION     XP_077502677.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646551.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..455
                     /product="DDB1- and CUL4-associated factor 11 isoform X5"
                     /calculated_mol_wt=51024
     Region          <101..433
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          143..184
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          191..227
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          232..274
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          281..316
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          322..355
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          363..403
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          409..433
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..455
                     /gene="LOC144113463"
                     /coded_by="XM_077646551.1:149..1516"
                     /db_xref="GeneID:144113463"
ORIGIN      
        1 mgrglmsrrc yfrlvsaass pdnsrilknd lytivnqqsg sknrgkpeqg tsmasllqqr
       61 ecgmpfrgif trgdkcrmss rflpnvtagt lfypsktfcg tfarngdifm tacqdcviri
      121 ydtshgdfde lkcvsardvg wsildmavsp dglyfayssw seylhlcnvy gdhqvheala
      181 lcpedrrfci fslqfscdgk dilggandsc lyvydreahh rslrirahdg dvnavafadk
      241 tsqilfsggd dglckvwdrr tlserdpkpv gilaghcngi tfvdskndar ylitnskdqt
      301 iklwdmrafs skegiqatkm avacqnwdyr wqrvpkklld lceklpgdts lmtyqghtvl
      361 qtlirchfsp iattgqqfiy tgcasgrvvv ynlltgkiet vlsghracvr dvswhpylpm
      421 lvssswdgsv tkwvynsepe skpvlkgklv eehne
//
LOCUS       XP_077502678             403 aa            linear   INV 25-NOV-2025
DEFINITION  DDB1- and CUL4-associated factor 11 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077502678
VERSION     XP_077502678.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646552.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..403
                     /product="DDB1- and CUL4-associated factor 11 isoform X6"
                     /calculated_mol_wt=45266
     Region          <49..381
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          91..133
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          139..175
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          180..222
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          229..264
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          270..303
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          311..351
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          357..381
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..403
                     /gene="LOC144113463"
                     /coded_by="XM_077646552.1:400..1611"
                     /db_xref="GeneID:144113463"
ORIGIN      
        1 masllqqrec gmpfrgiftr gdkcrmssrf lpnvtagtlf ypsktfcgtf arngdifmta
       61 cqdcviriyd tshgdfdelk cvsardvgws ildmavspdg lyfaysswse ylhlcnvygd
      121 hqvhealalc pedrrfcifs lqfscdgkdi lggandscly vydreahhrs lrirahdgdv
      181 navafadkts qilfsggddg lckvwdrrtl serdpkpvgi laghcngitf vdskndaryl
      241 itnskdqtik lwdmrafssk egiqatkmav acqnwdyrwq rvpkklldlc eklpgdtslm
      301 tyqghtvlqt lirchfspia ttgqqfiytg casgrvvvyn lltgkietvl sghracvrdv
      361 swhpylpmlv ssswdgsvtk wvynsepesk pvlkgklvee hne
//
LOCUS       XP_077502680             403 aa            linear   INV 25-NOV-2025
DEFINITION  DDB1- and CUL4-associated factor 11 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077502680
VERSION     XP_077502680.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646554.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..403
                     /product="DDB1- and CUL4-associated factor 11 isoform X6"
                     /calculated_mol_wt=45266
     Region          <49..381
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          91..133
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          139..175
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          180..222
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          229..264
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          270..303
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          311..351
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          357..381
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..403
                     /gene="LOC144113463"
                     /coded_by="XM_077646554.1:247..1458"
                     /db_xref="GeneID:144113463"
ORIGIN      
        1 masllqqrec gmpfrgiftr gdkcrmssrf lpnvtagtlf ypsktfcgtf arngdifmta
       61 cqdcviriyd tshgdfdelk cvsardvgws ildmavspdg lyfaysswse ylhlcnvygd
      121 hqvhealalc pedrrfcifs lqfscdgkdi lggandscly vydreahhrs lrirahdgdv
      181 navafadkts qilfsggddg lckvwdrrtl serdpkpvgi laghcngitf vdskndaryl
      241 itnskdqtik lwdmrafssk egiqatkmav acqnwdyrwq rvpkklldlc eklpgdtslm
      301 tyqghtvlqt lirchfspia ttgqqfiytg casgrvvvyn lltgkietvl sghracvrdv
      361 swhpylpmlv ssswdgsvtk wvynsepesk pvlkgklvee hne
//
LOCUS       XP_077502681             775 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113466 [Amblyomma americanum].
ACCESSION   XP_077502681
VERSION     XP_077502681.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646555.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..775
                     /product="uncharacterized protein LOC144113466"
                     /calculated_mol_wt=80607
     CDS             1..775
                     /gene="LOC144113466"
                     /coded_by="XM_077646555.1:150..2477"
                     /db_xref="GeneID:144113466"
ORIGIN      
        1 mtsrdqvgsd vadklssirl ptlpqqdgag yraasdhspa alghtshssc lpdksgggep
       61 elfttasdts slvapvcpqe ktaglrnlgs htagiqkptr dlcgfpggag asllqspavp
      121 setllmdlet pqqeqktsqy stnsndssik kpfsdvqglp kendlpenla gacglslelp
      181 gpsandiqgv vkpdideldf qgnsavankl slvrsakssp erledsqkkt sdtfaaaady
      241 isednlsigg kvtlqedsea drktpsvdip rtlqkeqgst entvdhhdav gapipchthe
      301 qsslssascv nllgpaegsl savlhnaqrm sklesserkd yhdsaptasc tseaslpira
      361 dvsfpensev nsntpsaeip stvetglrgt nrtvdghvea ggpcntnrvp slsstsedsv
      421 ldddedsmsa imriadamyk lptgspcpvt stignfqqif ispsttaakk tadvrgtaim
      481 pahsgdgsgr gtelaeipfm hysssgettw ggssyhvvpt asavplagvs sgaasqaglv
      541 sssavhehav altaersvag akkvehivvt lpsrsavpge ssqaietint raeglkrphs
      601 sagsenegls dfpsrlgshk klavikklcp edlsthvgrt srktkggaqa sqsagkgqqa
      661 amqsrqpaap rtgrkqvsgg qrkgkllskh pehmvpnvlh pvpsplssks scessklnnd
      721 gssgpsdtks apylsaklrs mrcstgqsss tpasnrassm ettrnrtqkk cslck
//
LOCUS       XP_077502682             709 aa            linear   INV 25-NOV-2025
DEFINITION  phosphorylated CTD-interacting factor 1 [Amblyomma americanum].
ACCESSION   XP_077502682
VERSION     XP_077502682.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646556.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..709
                     /product="phosphorylated CTD-interacting factor 1"
                     /calculated_mol_wt=80913
     Region          78..107
                     /region_name="WW"
                     /note="Two conserved tryptophans domain; also known as the
                     WWP or rsp5 domain; around 40 amino acids; functions as an
                     interaction module in a diverse set of signalling
                     proteins; binds specific proline-rich sequences but at low
                     affinities compared to other...; cd00201"
                     /db_xref="CDD:238122"
     Site            order(91,102)
                     /site_type="active"
                     /note="binding pocket [active]"
                     /db_xref="CDD:238122"
     Region          450..620
                     /region_name="PCIF1_WW"
                     /note="Phosphorylated CTD interacting factor 1 WW domain;
                     pfam12237"
                     /db_xref="CDD:463502"
     CDS             1..709
                     /gene="Pcif1"
                     /coded_by="XM_077646556.1:472..2601"
                     /db_xref="GeneID:144113468"
ORIGIN      
        1 mpemsdarfq nllsreeall drnhavneml skpaasweeq kfgvenvpvg qatpflsppq
       61 tvgpdpvhdl paellqigwr rfwskregrp yffnkltnes lwemprlstp tqydpltdpl
      121 giqgpptpvd pgtppapshl lpspppassp vllprpdkrr asddanpaaa kkfvlsgpfd
      181 levptnvviw ersptlmppp hpdvenlrst lttrlrtnyh emchsregie cpkesfirwl
      241 merkvvdkgh dpllpsvchp evsqcmyrei mndipiklvr pkfagdarkq lskyaeaakk
      301 miesrnaspe srkivkwnve dafhwlrktl natyddhler lahlkkqcqp hliqaarssv
      361 egicakifhm svdnvkkire khwsilnqhg ieeitsplhv tnpkkvfcyp vqfaissprl
      421 pavdvltdkd stllrykgev vrintlyfqk leqlyrwnct ddrkfenflp rvwcmvkryq
      481 tylglssneg hgtqnslpvl vmeclhkhfg vtfecfaspl ncyfrqycsa fpdtdsffgs
      541 rgtiisffpi agsfevnppf seelmeatvn hlerlltesq eslsfivffp dfrdpvpval
      601 tkleasrykr eqllipaydh eyrhgfqhim prsevsvrst hgthiiflqn dagyarwgpt
      661 perldalres yrpgldrdre vaaeavpvss lpskdtppkd lplgppkli
//
LOCUS       XP_077502683             877 aa            linear   INV 25-NOV-2025
DEFINITION  transcriptional repressor CTCF [Amblyomma americanum].
ACCESSION   XP_077502683
VERSION     XP_077502683.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646557.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..877
                     /product="transcriptional repressor CTCF"
                     /calculated_mol_wt=96230
     Region          <2..186
                     /region_name="COG4935"
                     /note="Regulatory P domain of the subtilisin-like
                     proprotein convertases and other proteases
                     [Posttranslational modification, protein turnover,
                     chaperones]"
                     /db_xref="CDD:443962"
     Region          334..354
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(339,341,343,345..346,349..350,353,367,369,373..374,
                     377..378,381,395,397,399,401..402,405..406,412)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          358..>648
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          362..382
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          390..413
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          419..439
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          447..467
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          475..495
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          504..525
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          533..553
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          561..581
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(566,568,570,572..573,576..577,580,594,596,600..601,
                     604..605,608,631,633,635,637..638,641..642,645)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          589..610
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          626..646
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..877
                     /gene="CTCF"
                     /coded_by="XM_077646557.1:225..2858"
                     /db_xref="GeneID:144113469"
ORIGIN      
        1 msdqqtdtpt dgassgpeaa sagdseaisd iqnylasfnk eiqgdgassa avaggdsait
       61 saetdtvete psvttadgtt tfyvtqtadg fqyatstqaq fdgttayytp aqatfyttdg
      121 eqaaegattv tgpgqletiq gyattadgka fpattttsgg lqqivlavgk gqgtesreia
      181 vtipdsigdq vqhesteell kaveqqgrsa gdavqpseqp tfvamqpgts hdgsdeqqvi
      241 lntgnsyqtv tivpsdtnpg evsyvlivsq pdeskedlak admdmsvydf heggdkagae
      301 ivaeeasape pvakmrtiki apkksqtvtq ahmcnycnyt spkryllsrh mkshseerph
      361 kcsvcergfk tlaslqnhvn thtgtrphqc kecdaaftts gelvrhvryr hthekphrct
      421 ecdyasvels klkrhmrcht gerpyqcphc tyaspdtykl krhlrihtge kpyecdvcha
      481 rftqsnslka hklihsgnkp ifqcelcptt cgrktdlrih vqklhtsdkp lkckrcgksf
      541 pdrytykvhv kshegekcfk cdlcpyasis qrhleshmli htdqkpfrcd ecdqafrqkq
      601 llkrhknlyh dpnyvppipk ekthecpecs kafrhkgnli rhlaihdpea taaeraeamr
      661 igepkgpgee pdgeeddyml dddedddmdd mamedgmvne gqqvvvfevi qipaadgvtm
      721 eqhairavse dalrmasngs vitgadgqel tiiqqvpqdq avvteavtka alapmkrprg
      781 rprkyatmea aasnqgeevl gmtkaemdap qreaemarrr leqkqkdmee cfgfndeede
      841 tilampaapg vngpsedkvq dgttttyilm qengdav
//
LOCUS       XP_077502684             877 aa            linear   INV 25-NOV-2025
DEFINITION  transcriptional repressor CTCF [Amblyomma americanum].
ACCESSION   XP_077502684
VERSION     XP_077502684.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646558.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..877
                     /product="transcriptional repressor CTCF"
                     /calculated_mol_wt=96230
     Region          <2..186
                     /region_name="COG4935"
                     /note="Regulatory P domain of the subtilisin-like
                     proprotein convertases and other proteases
                     [Posttranslational modification, protein turnover,
                     chaperones]"
                     /db_xref="CDD:443962"
     Region          334..354
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(339,341,343,345..346,349..350,353,367,369,373..374,
                     377..378,381,395,397,399,401..402,405..406,412)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          358..>648
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          362..382
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          390..413
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          419..439
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          447..467
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          475..495
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          504..525
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          533..553
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          561..581
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(566,568,570,572..573,576..577,580,594,596,600..601,
                     604..605,608,631,633,635,637..638,641..642,645)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          589..610
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          626..646
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..877
                     /gene="CTCF"
                     /coded_by="XM_077646558.1:796..3429"
                     /db_xref="GeneID:144113469"
ORIGIN      
        1 msdqqtdtpt dgassgpeaa sagdseaisd iqnylasfnk eiqgdgassa avaggdsait
       61 saetdtvete psvttadgtt tfyvtqtadg fqyatstqaq fdgttayytp aqatfyttdg
      121 eqaaegattv tgpgqletiq gyattadgka fpattttsgg lqqivlavgk gqgtesreia
      181 vtipdsigdq vqhesteell kaveqqgrsa gdavqpseqp tfvamqpgts hdgsdeqqvi
      241 lntgnsyqtv tivpsdtnpg evsyvlivsq pdeskedlak admdmsvydf heggdkagae
      301 ivaeeasape pvakmrtiki apkksqtvtq ahmcnycnyt spkryllsrh mkshseerph
      361 kcsvcergfk tlaslqnhvn thtgtrphqc kecdaaftts gelvrhvryr hthekphrct
      421 ecdyasvels klkrhmrcht gerpyqcphc tyaspdtykl krhlrihtge kpyecdvcha
      481 rftqsnslka hklihsgnkp ifqcelcptt cgrktdlrih vqklhtsdkp lkckrcgksf
      541 pdrytykvhv kshegekcfk cdlcpyasis qrhleshmli htdqkpfrcd ecdqafrqkq
      601 llkrhknlyh dpnyvppipk ekthecpecs kafrhkgnli rhlaihdpea taaeraeamr
      661 igepkgpgee pdgeeddyml dddedddmdd mamedgmvne gqqvvvfevi qipaadgvtm
      721 eqhairavse dalrmasngs vitgadgqel tiiqqvpqdq avvteavtka alapmkrprg
      781 rprkyatmea aasnqgeevl gmtkaemdap qreaemarrr leqkqkdmee cfgfndeede
      841 tilampaapg vngpsedkvq dgttttyilm qengdav
//
LOCUS       XP_077502685             877 aa            linear   INV 25-NOV-2025
DEFINITION  transcriptional repressor CTCF [Amblyomma americanum].
ACCESSION   XP_077502685
VERSION     XP_077502685.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646559.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..877
                     /product="transcriptional repressor CTCF"
                     /calculated_mol_wt=96230
     Region          <2..186
                     /region_name="COG4935"
                     /note="Regulatory P domain of the subtilisin-like
                     proprotein convertases and other proteases
                     [Posttranslational modification, protein turnover,
                     chaperones]"
                     /db_xref="CDD:443962"
     Region          334..354
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(339,341,343,345..346,349..350,353,367,369,373..374,
                     377..378,381,395,397,399,401..402,405..406,412)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          358..>648
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          362..382
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          390..413
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          419..439
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          447..467
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          475..495
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          504..525
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          533..553
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          561..581
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(566,568,570,572..573,576..577,580,594,596,600..601,
                     604..605,608,631,633,635,637..638,641..642,645)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          589..610
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          626..646
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..877
                     /gene="CTCF"
                     /coded_by="XM_077646559.1:409..3042"
                     /db_xref="GeneID:144113469"
ORIGIN      
        1 msdqqtdtpt dgassgpeaa sagdseaisd iqnylasfnk eiqgdgassa avaggdsait
       61 saetdtvete psvttadgtt tfyvtqtadg fqyatstqaq fdgttayytp aqatfyttdg
      121 eqaaegattv tgpgqletiq gyattadgka fpattttsgg lqqivlavgk gqgtesreia
      181 vtipdsigdq vqhesteell kaveqqgrsa gdavqpseqp tfvamqpgts hdgsdeqqvi
      241 lntgnsyqtv tivpsdtnpg evsyvlivsq pdeskedlak admdmsvydf heggdkagae
      301 ivaeeasape pvakmrtiki apkksqtvtq ahmcnycnyt spkryllsrh mkshseerph
      361 kcsvcergfk tlaslqnhvn thtgtrphqc kecdaaftts gelvrhvryr hthekphrct
      421 ecdyasvels klkrhmrcht gerpyqcphc tyaspdtykl krhlrihtge kpyecdvcha
      481 rftqsnslka hklihsgnkp ifqcelcptt cgrktdlrih vqklhtsdkp lkckrcgksf
      541 pdrytykvhv kshegekcfk cdlcpyasis qrhleshmli htdqkpfrcd ecdqafrqkq
      601 llkrhknlyh dpnyvppipk ekthecpecs kafrhkgnli rhlaihdpea taaeraeamr
      661 igepkgpgee pdgeeddyml dddedddmdd mamedgmvne gqqvvvfevi qipaadgvtm
      721 eqhairavse dalrmasngs vitgadgqel tiiqqvpqdq avvteavtka alapmkrprg
      781 rprkyatmea aasnqgeevl gmtkaemdap qreaemarrr leqkqkdmee cfgfndeede
      841 tilampaapg vngpsedkvq dgttttyilm qengdav
//
LOCUS       XP_077502686             674 aa            linear   INV 25-NOV-2025
DEFINITION  condensin-2 complex subunit H2-like [Amblyomma americanum].
ACCESSION   XP_077502686
VERSION     XP_077502686.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646560.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..674
                     /product="condensin-2 complex subunit H2-like"
                     /calculated_mol_wt=74928
     Region          72..190
                     /region_name="CNDH2_N"
                     /note="Condensin II complex subunit CAP-H2 or CNDH2,
                     N-terminal; pfam06278"
                     /db_xref="CDD:461866"
     Region          <428..635
                     /region_name="CNDH2_C"
                     /note="Condensin II complex subunit CAP-H2 or CNDH2,
                     C-term; pfam16858"
                     /db_xref="CDD:465283"
     CDS             1..674
                     /gene="LOC144113471"
                     /coded_by="XM_077646560.1:184..2208"
                     /db_xref="GeneID:144113471"
ORIGIN      
        1 masassavst lrvlpprmaa adtgapralk vqttaastsl sahviiaeap eadmlggpfl
       61 rqsardrkie rryvsllrpv rdltanfnvd ivthldqylr elsqtpvtyk egepsvnftl
      121 aafvidgsas vygrkvdhlh tlvrkvagev ahrtseghna qnadhhsrga rpktspkrsn
      181 refqtfdeie ladelnimpr pqqapgdlaa aqvnrflrrp lsmvmlsaeg etelrdlrgd
      241 ligykegyrl ystskhlegc eyadeecghs sdhargslpa vgfsldveyd cardsslatp
      301 hlpgtippcr ysggseelpa radsevsfpa pvpqareqek gqkpfigvkt cidsqeevps
      361 kcrpmlvrsk arkracdest stdgcfvpia atwvnstqyp hlfhtelqlr kremvipgge
      421 rylrdsmkcl kmkkelredy cssqpqeegf ddsdrgvndg aggcfeasdm qpgdlvvmee
      481 dkdvgpvsrm dagvvrreaq rptspvdsse gdsrqkvrhs ieceldtpms efqhldlqka
      541 vaewdnrirp lldtqekres fnihtycsri ldhfsdtpsk qtlffrdicq gqpgwkvarn
      601 fvaalelans ynvelgtndv leegmdtlhl tllsrkqnfh eleksdsqvs mtarlsqtse
      661 kstqiskrph padl
//
LOCUS       XP_077502687             433 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-1,3-mannosyl-glycoprotein
            2-beta-N-acetylglucosaminyltransferase-like [Amblyomma americanum].
ACCESSION   XP_077502687
VERSION     XP_077502687.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646561.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..433
                     /product="alpha-1,3-mannosyl-glycoprotein
                     2-beta-N-acetylglucosaminyltransferase-like"
                     /calculated_mol_wt=50801
     Region          76..407
                     /region_name="Glyco_tranf_GTA_type"
                     /note="Glycosyltransferase family A (GT-A) includes
                     diverse families of glycosyl transferases with a common
                     GT-A type structural fold; cl11394"
                     /db_xref="CDD:472172"
     Site            order(82,84,111,183,185)
                     /site_type="active"
                     /db_xref="CDD:133007"
     CDS             1..433
                     /gene="LOC144113472"
                     /coded_by="XM_077646561.1:268..1569"
                     /db_xref="GeneID:144113472"
ORIGIN      
        1 mnrlhvlllv gallwafvtg lifvqisqsr lhaiirsqaa vlerdvrsql hsndevldrl
       61 krwkklidda rhrqevitvl vlacnrvsvk rtldqvvryl psqrqfpviv sqdgyhnett
      121 qlllqyqreh nftlmhqpdq tdltagvtav fniegyyris rhykwaltqv fdrlersavi
      181 vleddldiap dffeyfasll pilrkdptlf cvsayndngr rthisllpdm lhrtdffsgl
      241 gwlltrelwq eigprwpkaf wddwirqpeq rrgracirpe vprtrtygrd gvsegqffdk
      301 ylkdihlnev fvrfssmdln yllqsrydta fqqtvsesrm ytlenlrdgl itqtparipy
      361 tkegdfdeia kvlhimsdfk sgvprtaykg vvsiyldgir lfivphpwpf ehemhllekn
      421 rhelngplvr plr
//
LOCUS       XP_077502689             355 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113473 [Amblyomma americanum].
ACCESSION   XP_077502689
VERSION     XP_077502689.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646563.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..355
                     /product="uncharacterized protein LOC144113473"
                     /calculated_mol_wt=38801
     Region          209..229
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          <212..338
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          240..260
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          264..286
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          266..286
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(271,273,275,277..278,281..282,285,299,301,305..306,
                     309..310,313,327,329,331,333..334,337..338)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          279..302
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          294..314
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          322..338
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..355
                     /gene="LOC144113473"
                     /coded_by="XM_077646563.1:148..1215"
                     /db_xref="GeneID:144113473"
ORIGIN      
        1 mprsflikkh vnagaaavsr pvafvkrpwl degsveaedl stspppavrv plalspalsd
       61 gcvpdiarpf dlslkakkps grpeypsihr pvaitpppgv aliapkgdrp ldwphqpayl
      121 thsympltly pvppatdyya plvppplvag hpltprelak qsppqrdavv pswssgvsss
      181 ssdekesrgs papperprrr penaprypct gcnrsystys glskhrqfhc sattsksfsc
      241 kycekdyssl galkmhirth tlpckcklcg kafsrpwllq ghirthtgek pfacphcsra
      301 fadrsnlrah lqthsdikky sckscsrtfs rmslllkhed ggcpaaalnr sqpkp
//
LOCUS       XP_077502690             796 aa            linear   INV 25-NOV-2025
DEFINITION  bromodomain-containing protein 7-like [Amblyomma americanum].
ACCESSION   XP_077502690
VERSION     XP_077502690.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646564.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..796
                     /product="bromodomain-containing protein 7-like"
                     /calculated_mol_wt=88354
     Region          203..298
                     /region_name="Bromodomain"
                     /note="Bromodomains are found in many chromatin-associated
                     proteins and in nuclear histone acetyltransferases. They
                     interact specifically with acetylated lysine; cl02556"
                     /db_xref="CDD:445827"
     Site            order(228,233,236,275,279,285)
                     /site_type="active"
                     /note="acetyllysine binding site [active]"
                     /db_xref="CDD:99945"
     Region          374..550
                     /region_name="DUF3512"
                     /note="Domain of unknown function (DUF3512); pfam12024"
                     /db_xref="CDD:463436"
     CDS             1..796
                     /gene="LOC144113474"
                     /coded_by="XM_077646564.1:116..2506"
                     /db_xref="GeneID:144113474"
ORIGIN      
        1 mgskkhkkhh ksekkeakee drqekslklv lkvggstggl qtpvpspsav asphntvpps
       61 qqqhepmpaa aapaafrveg tsvvchaheg ssskhkkskk kkkkkssske hrekrhrhhh
      121 hrhhhhhhhh hkdsekkekr kcehdcslgt derqsvpsav epplakrprq eptpvppeps
      181 pppaerpvrd prtctlkksd ktplqmllyy llknlqkrdp qdffawpvnd iiapgystii
      241 hspmdfstmr kkiddndytc vsefrddlkl mcdnamtynr adtvyyksak rmwysgnkll
      301 lkdhllgleg tlpffpdlts eelgfdvdgt asapdikplp dvadapvtie aaplgspeya
      361 kktkngssed gasddqaane illqaqkaaq vaagrlslkk vpskfgflrq mddgsttlai
      421 lnpdygslkg pkeikvnlem lvgklahgtg tlmdfkddik niarpinyln ygtystyaph
      481 ydssfanlsk eesdlvysty gdelgvqyae sllsfakdcd yvmdmvdnll dvmtsgehsk
      541 tvkileahrn givpeekdla sftnhaevee veveqpepkc sdsrpsdsfy slndlaadst
      601 vvdsnhaslp qihqsfterd lvlgnthevh ehhdhhehhe hhehhdrhdh lhqtvsesss
      661 ldsadsdina vlqqklqesk emidtlsrlq qerlsatppa hlshiqgase lelelaekia
      721 ktlselasha kpgsivsvea irkamginyp ptgtchhhhs cphvaspysv sahdlpdsss
      781 sndvslrand spssnt
//
LOCUS       XP_077502691             204 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113475 [Amblyomma americanum].
ACCESSION   XP_077502691
VERSION     XP_077502691.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646565.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..204
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..204
                     /product="uncharacterized protein LOC144113475"
                     /calculated_mol_wt=21504
     Region          38..89
                     /region_name="Chitin_bind_4"
                     /note="Insect cuticle protein; pfam00379"
                     /db_xref="CDD:459790"
     CDS             1..204
                     /gene="LOC144113475"
                     /coded_by="XM_077646565.1:156..770"
                     /db_xref="GeneID:144113475"
ORIGIN      
        1 mnpkllvmlg tlavvnagvp llggestqhr aqddygnyqf sydivdplga tngrwevgda
       61 ygnkrggyti tdidgrqrrv eyvadaygfr vivntnepgt aasapasavy nspyvnvirg
      121 lalarpvvaa aasaavpvas vhapyvkrvr kvfkvpvlqa paaavppvvl aapplggqyp
      181 gyapsihgqf gkrigaarnf ynyy
//
LOCUS       XP_077502692             444 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113476 [Amblyomma americanum].
ACCESSION   XP_077502692
VERSION     XP_077502692.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646566.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..444
                     /product="uncharacterized protein LOC144113476"
                     /calculated_mol_wt=51337
     Region          71..>143
                     /region_name="PUB"
                     /note="PNGase/UBA or UBX (PUB) domain of p97 adaptor
                     proteins; cl15262"
                     /db_xref="CDD:472791"
     Site            order(84,87..88,91,99..100,105..107,110,112,116)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:198416"
     CDS             1..444
                     /gene="LOC144113476"
                     /coded_by="XM_077646566.1:438..1772"
                     /db_xref="GeneID:144113476"
ORIGIN      
        1 msepkispsd plklwysikg dvrtaksval esltlqsvdh svyvmlqmnc dfikkfefln
       61 iseiivtnlr ngmslmeisd clrilakyak emlkptkery khwnkipfmk agapqkwtrm
      121 dgtktilkql gytqetskgy ifplhsfgpp lnvikslsle lalaaaelsv fynnhhphpe
      181 yirnimcpgg allpsasste rrefnvmdll aevgnsanlr dltqapipaq nlmspsphkg
      241 rgrppavtra sevvancaaa cnchayydny taqqqqqqqq qqqqqqqqqq qqqqqqqlpa
      301 hqqplhlqtp shlqpahqqq qelqrhqqei qrhqqemqqq emqqqqqqqq qqqqqqqqqq
      361 qqqqqqqqqq qqqqqqqqqq qqadqqaqpq qqqqqqqppq qqqqqppqqq pqqqpppqqp
      421 qqdiqqqqqq qqpppppppr epqp
//
LOCUS       XP_077502693             225 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113477 [Amblyomma americanum].
ACCESSION   XP_077502693
VERSION     XP_077502693.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646567.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..225
                     /product="uncharacterized protein LOC144113477"
                     /calculated_mol_wt=24278
     CDS             1..225
                     /gene="LOC144113477"
                     /coded_by="XM_077646567.1:35..712"
                     /db_xref="GeneID:144113477"
ORIGIN      
        1 mrqfcacvai tgcadngppp qlfkqscswn kallilcivt hasvcysgde arsnpckgll
       61 vgdmafgsri refdlsenss wvdyveriel scaanklttd ddkravllsc cgpetyslia
      121 tlvkpsrppn vgyqvivdav kkhinpkpse lysstlrrtp rpgnlqpksc wagssgppyp
      181 afarmrvtqa afgsrhhevs slvtpctrgt sgqgpsgclh acrdr
//
LOCUS       XP_077502694             806 aa            linear   INV 25-NOV-2025
DEFINITION  leucine-rich repeat-containing G protein-coupled receptor 1
            [Amblyomma americanum].
ACCESSION   XP_077502694
VERSION     XP_077502694.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646568.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..806
                     /product="leucine-rich repeat-containing G protein-coupled
                     receptor 1"
                     /calculated_mol_wt=89948
     Region          73..97
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <82..>273
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          98..122
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          123..147
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          148..162
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          197..217
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          218..242
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          243..263
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          468..721
                     /region_name="7tmA_Glyco_hormone_R"
                     /note="glycoprotein hormone receptors, member of the class
                     A family of seven-transmembrane G protein-coupled
                     receptors; cd15136"
                     /db_xref="CDD:320264"
     Region          479..504
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320264"
     Site            order(500,503..504,524..529,531..532,535,580,582..586,608,
                     611..613,615..617,619..620,669,672..673,675..676,679,
                     688..689,691..693,696,699..700)
                     /site_type="other"
                     /note="putative peptide ligand binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:320264"
     Region          524..554
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320264"
     Region          566..588
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320264"
     Region          608..637
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320264"
     Region          647..679
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320264"
     Region          689..714
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320264"
     CDS             1..806
                     /gene="Lgr1"
                     /coded_by="XM_077646568.1:16..2436"
                     /db_xref="GeneID:144113478"
ORIGIN      
        1 megrratrll allvlvvlas avggplsaag tqlpvpftet aaaegcrcwa qgtecrcfgd
       61 svrevpaqlt drveklsler isldvlrngs lesyadtlra lhlnsllklr fiepgvfdnl
      121 helreisiqk simlqsvppg vlrglpklsv lrithsglre vpymhdlnps tyigmldfds
      181 nriaelaans ikvkagtlil nhntiaaira aafdgskiae vrlrgnrklt vveddafygl
      241 snlshldlse tsltklptrg leslevlelr dapalhefps vyhfrfirva hltypyhcca
      301 fqfpethnpe ehsrfqehcd sedraamsrr qnrppaigee hdfqllerff vvqlsnaltt
      361 tqptrrfvrh vfsefgtihe spksrvsnvs sspwnifdsr dmegtfhpev rpqeeqwvtc
      421 gklasrrvqc lpapdafnpc edvmgtvvlr vavwfvvvaa vagnlavllv llsgrlsvsk
      481 flmcnlafad lcmgayllli aaedlhtrgr yfshailwqh gagckvagfl tvfaselsiy
      541 tltvitlerw yaityavhln rrlrlrtaar imacgwlyal laaslpllgi ssysktsicl
      601 pmenstvadl lylltllsvn glafvlicgc yakmylaisg qrprshcgnk dtsvakrmam
      661 lvftdfacwa piaffgltav agyplidvpk skillvffyp lnscanpfly ailtkqyrrd
      721 laallstrgv ctrrmlkyts swhttptnrl lrnkdnssts rqttelsnrt qkgsphpikn
      781 nftklgvhee flrrqsllce hldtpi
//
LOCUS       XP_077502695            2275 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC144113479
            [Amblyomma americanum].
ACCESSION   XP_077502695
VERSION     XP_077502695.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646569.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2275
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC144113479"
                     /calculated_mol_wt=250022
     Region          25..68
                     /region_name="Bbox1_HOIP"
                     /note="B-box-type 1 zinc finger found in
                     HOIL-1-interacting protein (HOIP) and similar proteins;
                     cd19815"
                     /db_xref="CDD:380873"
     Region          249..>604
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     Region          606..626
                     /region_name="ZnF_RBZ"
                     /note="Zinc finger domain; smart00547"
                     /db_xref="CDD:197784"
     Region          606..625
                     /region_name="RanBP2-type Zn finger"
                     /note="RanBP2-type Zn finger [structural motif]"
                     /db_xref="CDD:275376"
     Region          1006..1155
                     /region_name="HOIP-UBA"
                     /note="HOIP UBA domain pair; pfam16678"
                     /db_xref="CDD:465229"
     Region          1906..1959
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(1908,1911,1926,1928,1931,1934,1953,1956)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438293"
     Region          1984..2062
                     /region_name="BRcat_RBR_HOIP"
                     /note="BRcat domain found in HOIL-1-interacting protein
                     (HOIP) and similar proteins; cd20337"
                     /db_xref="CDD:438998"
     Site            order(1989,1991..1996,1998..1999,2007..2009,2011..2012,
                     2014..2020,2026,2028..2029,2039..2040,2044..2045,2049,
                     2053,2055,2057,2060..2062)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:438998"
     Region          2063..2147
                     /region_name="Rcat_RBR_HOIP"
                     /note="Rcat domain found in HOIL-1-interacting protein
                     (HOIP) and similar proteins; cd20351"
                     /db_xref="CDD:439012"
     Site            order(2064..2066,2069..2070,2073..2074,2086..2091,
                     2093..2095,2097..2099,2101..2102,2104,2106,2115,2118,2129,
                     2131..2133,2136,2142,2145..2146)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:439012"
     Region          2150..2240
                     /region_name="E3_UbLigase_RBR"
                     /note="E3 Ubiquitin Ligase RBR C-terminal domain;
                     pfam18091"
                     /db_xref="CDD:436265"
     CDS             1..2275
                     /gene="LOC144113479"
                     /coded_by="XM_077646569.1:308..7135"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:144113479"
ORIGIN      
        1 mfarkdvgap ngvgaagpsa rdgshcdlcg ctrpsvrcdk cnrqvfclsc ddmyhrhprr
       61 kshlrravds vnsgrpvavk ppqqrvegdp shmpvppprk kkslfsafsr gpaathltdd
      121 lerhpalpkk efswtdkfgs ikrfmasrpl ppvppedegl pppspryhrd hvplnyvnvd
      181 pnppavpqrp pprlgfpppp pqqqqqqqlh rdrrelahmg vggarndrmt tlddwddigs
      241 asasshgsdd eswgpegyrr pgaehrdgpp frkvgppaaa hpafgtlrkh ssphafavhk
      301 ggesstlnrq sssmtdlhgl hagypgsaaa mippgpgypa gpafrapfgy ppmaayplps
      361 atalgyyrpp gaflggstgs wsnvnsaaed gketdddksq rhstldcrri krsqscmhdp
      421 mmhpapfypf gypaypplfp pwmrppspsp sqhslpvada sgdrlhrphh rkhkkslskt
      481 tsrrqssqel ssdedqdsps lddhssrdrg shssekaksr grtpssrdrd dakapdkakt
      541 etcgtptege hsdavrqqqa rllaaaarge qqrsqtpaeq apaglqqgap epsalpqpkw
      601 dpskpwrcqh ctfineagsr iclicckttf reqspdaasd deerqkkrag fqdtladkqi
      661 elssaksdve ptkseaapae sqpeakpean pgpspdddnq vnftdefike qqevekeirr
      721 rleiqmride enrriesagv vqesqtvqhf qgdsaetess smapqqamal caevgstpla
      781 skaaislatq papsyqssvq qchsssqtva ggherspaas dfvdttptit tqavrpkvly
      841 kasvatdted ftdpstlspl sdaqqeiqer rrvfaeakaq sldqprpafl qrkgeqaapk
      901 gtrignlirt lsktslqnte pegrlyrshs rsslhsdmsd aspvrrgiar hgslaefdrp
      961 fglenrkddd asslvgatss syisegrkqd yylsleelvq qrkqeqmrtq glelvrlire
     1021 aeqrgftadd lqvamnhcgn dnpinwlrdn wqnmtetvvt latnyghdrr ennigivsas
     1081 eaqnalrrqk gniwaavtec venrqrmfme lssrgnftrq eilaaltdnh gnmdmayaql
     1141 tkatlkpflm riwgpgagad ndegrrpspl sdkreesvtd rvkdwlesld aasqltssna
     1201 stleerrakr hsfhgcrseq lfsdgshrdr stspassfss lretsevssv ssatqgtssr
     1261 ieailrrrea qkefekgrks kkeahadgsk rrwtlasvfn akkpgqttkr dtfaaagssa
     1321 eksssqttlt sdttqldsss sgatvsmetl asieatgakp ktflgrfssf knesrkkesk
     1381 ateaigsdna dgfpkafptg dvpraetqnl qfkretskdd pnsncatkae tnqahveass
     1441 shdaservda amgnvpfsan rnaienikqs ihaaklsfma sapgaivhnt sadasglstn
     1501 frnsqnsdla egeatalaak avekstesre erpdnkvkse lkergppasq vkeksndtna
     1561 assssshvss slignqptes tnisevgkst navsklltss ttqaaidasg sneqpaqket
     1621 mseggelsta savapkvvvh sadtsenhyd aaapapsads ssksgsytqt ptkggkqtaa
     1681 vgdpktanay stesqerndk eetraggnes egaalgattt rklgnrlpea atprrrervl
     1741 tngtaegveq etlkpsaagk ggpnrrpslr rtsrensddc vvrdrdgnal ivkpqkpier
     1801 rgsvnarpgl qiskavaani aklrsvpppk krkqvvqprp agklptekqk qykakaeelv
     1861 legrcptmvh ahlvaelidm sfdeedaila aeqcesiyqa vnflqqecel caanypisqm
     1921 vsllncvhra ckeclkayft iqirdrnime llcpfcnepd lsdedvelny fnnldillks
     1981 fverdvydlf qqklrdralm kdpnfrwcsq vvlfgfityp nqtrlvcpdc kavtcascrk
     2041 pwqkqhegit ceqfqewqqd ndpesqaras rdiwlkngid cpnckfryal arggcmhfkc
     2101 yqcsfdfccg cnlpfkmgek cgrtpncarl glhahhprnc lfylrdknie dlqrlleesn
     2161 vpfnrappah wvktsrcevp eqkesvdgfk ddicgrpvee gtaglcrmhy veylgqlifk
     2221 hhidplpifd vdelelvlkr anvrlpsryk lsdreyqdal ikvidtdvpl dvaag
//
LOCUS       XP_077502696             343 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase RNF31-like [Amblyomma americanum].
ACCESSION   XP_077502696
VERSION     XP_077502696.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646570.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..343
                     /product="E3 ubiquitin-protein ligase RNF31-like"
                     /calculated_mol_wt=39254
     Region          64..141
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(86,91,106,109,114,117)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     Region          142..225
                     /region_name="BRcat_Rcat_RBR"
                     /note="BRcat (benign-catalytic) and Rcat
                     (required-for-catalysis) domains, part of the RBR
                     (RING1-BRcat-Rcat) domain; cl45895"
                     /db_xref="CDD:459240"
     Site            order(160,163,179,182,187,190)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438995"
     Region          228..311
                     /region_name="E3_UbLigase_RBR"
                     /note="E3 Ubiquitin Ligase RBR C-terminal domain;
                     pfam18091"
                     /db_xref="CDD:436265"
     CDS             1..343
                     /gene="LOC144113482"
                     /coded_by="XM_077646570.1:188..1219"
                     /db_xref="GeneID:144113482"
ORIGIN      
        1 mnmfpecdld rnklrpcqht gcsvclkahf wngaengqls clqcdaeilq henicllrsi
       61 fggeyddfdr rllersierq seyvycinek cgrcfnvpeg ltrldcpfcr tvacalcksk
      121 wrkeherrsc eefkkwkaen dpddpefqtq dlirrtaiec prcqtqyfka kggcahftcg
      181 nckrefcecc ktefwrgkec greacknqgm hghhprncff ytrdysheel lgilknasig
      241 vdeeipadst ltcsvsitnd dfhdspcgla vlrakkcekh ykeylcesif ahrvdvlslm
      301 neqkleqelh kngvnvpvip aanpgeklqa lrqavaaavp lrs
//
LOCUS       XP_077502697             842 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113483 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502697
VERSION     XP_077502697.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646571.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..842
                     /product="uncharacterized protein LOC144113483 isoform X1"
                     /calculated_mol_wt=91722
     Region          242..262
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(242,245,258,262)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          270..291
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          453..474
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          479..497
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..842
                     /gene="LOC144113483"
                     /coded_by="XM_077646571.1:286..2814"
                     /db_xref="GeneID:144113483"
ORIGIN      
        1 mdkgtlnaaf sclegiytnq nvgkhvsall qasrgsashf lppyplfppl applpatddc
       61 akkahlpqpq qpqqpqqpqq qqqqhsrvps sgtsakserk tkrdpgassn dgwvqgqekf
      121 swdgspvaay npatkmfrcv acntlgflsr iaehylgthv nakqvfqcpe cpyssawsrc
      181 vrlhlgkvhg iqnapaslwr dqplldeimg llqnlknlae asggagltaa patpavdkry
      241 vcprcpyatd rrdlyhrhen ihkddkpfhc yvcfklfnra dhvkkhflri hknhpydigl
      301 irrhpprsas haahgpsasg hhhsnngqwg ahaagtarlp qpsnctgpad csralpvpan
      361 gcpaggtqaw gfhasgvpav csgggpkamg cptalpqeta akgkrggsak aaatatsgta
      421 aaktfacayc pwrgvdgwcl rrhlnthikp fscpfceyka arserlnthv lkvhgkhlcn
      481 kchfagedsi alerhvkenh lssslndksr mgcslyppfp vrpphpsisq pvdkckiepc
      541 gcsvtngtsa cssnskpqvg pteqrgladc vspgprhdpa qeggcrtade rplgqgglat
      601 vfpyrcvvcg fcsftqdtmi ahmrlhsgqt lccramdacc qystpfedvl rkhvstehad
      661 dsavarcphc gmpcssakvl trhaaeshhn mvlrgrcadc avmllrhfgl vaepdatgrv
      721 vaapvlqvqr lkqsrkqpkp rkvvvccsdr cgksqlgpaa prrrrkhcap prlplgkpsl
      781 slschhckle gglrrkkafr chssylvhmy wchsrerhrc srcglgfrhg yqsylhskkq
      841 hs
//
LOCUS       XP_077502698             841 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113483 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502698
VERSION     XP_077502698.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646572.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..841
                     /product="uncharacterized protein LOC144113483 isoform X2"
                     /calculated_mol_wt=91594
     Region          241..261
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(241,244,257,261)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          269..290
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          452..473
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          478..496
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..841
                     /gene="LOC144113483"
                     /coded_by="XM_077646572.1:261..2786"
                     /db_xref="GeneID:144113483"
ORIGIN      
        1 mdkgtlnaaf sclegiytnq nvgkhvsall qasrgsashf lppyplfppl applpatddc
       61 akkahlpqpq qpqqpqqpqq qqqqhsrvps sgtsakserk tkrdpgassn dgwvqgqekf
      121 swdgspvaay npatkmfrcv acntlgflsr iaehylgthv nakvfqcpec pyssawsrcv
      181 rlhlgkvhgi qnapaslwrd qplldeimgl lqnlknlaea sggagltaap atpavdkryv
      241 cprcpyatdr rdlyhrheni hkddkpfhcy vcfklfnrad hvkkhflrih knhpydigli
      301 rrhpprsash aahgpsasgh hhsnngqwga haagtarlpq psnctgpadc sralpvpang
      361 cpaggtqawg fhasgvpavc sgggpkamgc ptalpqetaa kgkrggsaka aatatsgtaa
      421 aktfacaycp wrgvdgwclr rhlnthikpf scpfceykaa rserlnthvl kvhgkhlcnk
      481 chfagedsia lerhvkenhl ssslndksrm gcslyppfpv rpphpsisqp vdkckiepcg
      541 csvtngtsac ssnskpqvgp teqrgladcv spgprhdpaq eggcrtader plgqgglatv
      601 fpyrcvvcgf csftqdtmia hmrlhsgqtl ccramdaccq ystpfedvlr khvstehadd
      661 savarcphcg mpcssakvlt rhaaeshhnm vlrgrcadca vmllrhfglv aepdatgrvv
      721 aapvlqvqrl kqsrkqpkpr kvvvccsdrc gksqlgpaap rrrrkhcapp rlplgkpsls
      781 lschhckleg glrrkkafrc hssylvhmyw chsrerhrcs rcglgfrhgy qsylhskkqh
      841 s
//
LOCUS       XP_077502700             555 aa            linear   INV 25-NOV-2025
DEFINITION  ATP synthase F(1) complex subunit alpha, mitochondrial-like
            [Amblyomma americanum].
ACCESSION   XP_077502700
VERSION     XP_077502700.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646574.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..555
                     /product="ATP synthase F(1) complex subunit alpha,
                     mitochondrial-like"
                     /calculated_mol_wt=59838
     Region          50..555
                     /region_name="PRK09281"
                     /note="F0F1 ATP synthase subunit alpha; Validated"
                     /db_xref="CDD:236448"
     CDS             1..555
                     /gene="LOC144113484"
                     /coded_by="XM_077646574.1:1..1668"
                     /db_xref="GeneID:144113484"
ORIGIN      
        1 mafvsrrlac tlarhfpkqa sklapaheai salalrklst tpcsyaaags aevstileer
       61 ilgqasttnl eetgrvlsig dgiarvyglk niqaeemvef ssglkgmaln lepdnvgivv
      121 fgndklikeg divkrtgaiv dvpvgpellg rvvdalgnpi dgkgpvacks rarvgvkapg
      181 iiprisvkep mltgikavds lvpigrgqre liigdrqtgk taiaidgiin qkrfndgsde
      241 kkklyciyva igqkrstvaq ivkrltqsda mkytiivsat asdaaplqyl apysgcamge
      301 yfrdngmhal iiyddlskqa vayrqmslll rrppgreayp gdvfylhsrl leraakmnda
      361 fgggsltalp vietqagdvs ayiptnvisi tdgqiflete lfykgirpai nvglsvsrvg
      421 saaqtkamkq vagsmklela qyrevaafaq fgsdldaatq qllnrgvrlt ellkqgqytp
      481 maiedqvavi ytgvrgyldk ldpshinefe keflqhvrsh hkdilttiaq eghisestda
      541 klksivtsfv anfsa
//
LOCUS       XP_077502701             300 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113485 [Amblyomma americanum].
ACCESSION   XP_077502701
VERSION     XP_077502701.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646575.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..300
                     /product="uncharacterized protein LOC144113485"
                     /calculated_mol_wt=33021
     Region          21..>204
                     /region_name="PHA03384"
                     /note="early DNA-binding protein E2A; Provisional"
                     /db_xref="CDD:223068"
     Region          <134..279
                     /region_name="JHBP"
                     /note="Haemolymph juvenile hormone binding protein (JHBP);
                     cl12117"
                     /db_xref="CDD:472279"
     CDS             1..300
                     /gene="LOC144113485"
                     /coded_by="XM_077646575.1:170..1072"
                     /db_xref="GeneID:144113485"
ORIGIN      
        1 magvkksrgp qppqpkrgpp rratarvapk gdrsgdkpgh kpddkpgdkp gyksgdkpgh
       61 kpsdkpgdkp gdkpgdksgd kpghksgdkp gdkqkdegyg dvcevigekl lkllsdgaer
      121 alhsiplpll gasdaelrda tllgldnfkl rpedparvvr sekhlivhip lqvqdlnvtg
      181 kyttlrshvt mkgkfkahlq hlhihteivi csgrpklqtl evtkmeglri tsakgmtvfl
      241 nwvlvalids finrnqarff rvirealwka famhvmtvlp rldvryiskk qachhgqqkn
//
LOCUS       XP_077502702             300 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113485 [Amblyomma americanum].
ACCESSION   XP_077502702
VERSION     XP_077502702.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646576.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..300
                     /product="uncharacterized protein LOC144113485"
                     /calculated_mol_wt=33021
     Region          21..>204
                     /region_name="PHA03384"
                     /note="early DNA-binding protein E2A; Provisional"
                     /db_xref="CDD:223068"
     Region          <134..279
                     /region_name="JHBP"
                     /note="Haemolymph juvenile hormone binding protein (JHBP);
                     cl12117"
                     /db_xref="CDD:472279"
     CDS             1..300
                     /gene="LOC144113485"
                     /coded_by="XM_077646576.1:49..951"
                     /db_xref="GeneID:144113485"
ORIGIN      
        1 magvkksrgp qppqpkrgpp rratarvapk gdrsgdkpgh kpddkpgdkp gyksgdkpgh
       61 kpsdkpgdkp gdkpgdksgd kpghksgdkp gdkqkdegyg dvcevigekl lkllsdgaer
      121 alhsiplpll gasdaelrda tllgldnfkl rpedparvvr sekhlivhip lqvqdlnvtg
      181 kyttlrshvt mkgkfkahlq hlhihteivi csgrpklqtl evtkmeglri tsakgmtvfl
      241 nwvlvalids finrnqarff rvirealwka famhvmtvlp rldvryiskk qachhgqqkn
//
LOCUS       XP_077502703             427 aa            linear   INV 25-NOV-2025
DEFINITION  carboxypeptidase B-like [Amblyomma americanum].
ACCESSION   XP_077502703
VERSION     XP_077502703.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646577.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..427
                     /product="carboxypeptidase B-like"
                     /calculated_mol_wt=48145
     Region          38..110
                     /region_name="Propep_M14"
                     /note="Carboxypeptidase activation peptide; pfam02244"
                     /db_xref="CDD:460505"
     Region          149..424
                     /region_name="Peptidase_M14_like"
                     /note="M14 family of metallocarboxypeptidases and related
                     proteins; cl11393"
                     /db_xref="CDD:472171"
     Site            order(190,193,248,268..269,321..322,328,396)
                     /site_type="active"
                     /db_xref="CDD:349427"
     CDS             1..427
                     /gene="LOC144113486"
                     /coded_by="XM_077646577.1:412..1695"
                     /db_xref="GeneID:144113486"
ORIGIN      
        1 mamsaalpll llaclawpra qgalegatdt nffghhllsv ridtlshlkt lldvkdsldl
       61 dfwshtireg ytglvrvppe svneivdvme sagiqynvns dnlqslinee krliapddas
      121 pagesstdll dysryysyrs vtkallgyak ahsfletqvi gqthddndii svkiaseadl
      181 pvividcgvh arewvtisac myiinelvtr hqeratdvsa ifnyewrvip clnpdgyefs
      241 rrsnrmwrkn rsvqysvngt kcygtdlnrn fdvsfcstve eadpcketfc gkapfsepes
      301 hafreyvtef rdriafyfsf hafgdlwlip ysfrterppd ydelmaranr avdaiqqvsg
      361 tryragsias llyiapgtsi dwmyvkkgvr msftievsse kngfflnqsn vmevpkqawv
      421 gmvaaiq
//
LOCUS       XP_077502704             300 aa            linear   INV 25-NOV-2025
DEFINITION  T-box transcription factor TBX20-like [Amblyomma americanum].
ACCESSION   XP_077502704
VERSION     XP_077502704.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646578.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..300
                     /product="T-box transcription factor TBX20-like"
                     /calculated_mol_wt=34058
     Region          1..163
                     /region_name="T-box"
                     /note="DNA-binding domain of the T-box transcription
                     factor family; cl00145"
                     /db_xref="CDD:469628"
     Site            order(33,79,82,95..96,139..142,147,150..151,154)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410319"
     CDS             1..300
                     /gene="LOC144113487"
                     /coded_by="XM_077646578.1:334..1236"
                     /db_xref="GeneID:144113487"
ORIGIN      
        1 mfptvrvsfs gvqpdaryiv lldivpvdnk ryryayhrss wlvagkadpp sparlyahpd
       61 cpftgdqlrk qvvsfekvkl tnnemdkqgh ivlnsmhkyq prihlvkrrh napnvpvndl
      121 eaeqfrtyvf petvftavta yqnqlitklk idsnpfakgf rdssrltele retmeslitd
      181 hpfartplrt flenggdpet aaylhekgll aawgprlqrr pwrgprcsgs rrawsctrsl
      241 rstrsacapr rprasrpscg pvgggrgrrg vaaapgldae rrrgrprqrr rrpavrssrp
//
LOCUS       XP_077502705             564 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113488 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502705
VERSION     XP_077502705.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646579.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..564
                     /product="uncharacterized protein LOC144113488 isoform X1"
                     /calculated_mol_wt=60722
     Region          176..369
                     /region_name="T-box_TBX20-like"
                     /note="DNA-binding domain of T-box transcription factor 20
                     and related T-box proteins; cd20193"
                     /db_xref="CDD:410319"
     Site            order(203..204,237,283,286,299..300,345..348,353,356..357,
                     360..366)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410319"
     CDS             1..564
                     /gene="LOC144113488"
                     /coded_by="XM_077646579.1:136..1830"
                     /db_xref="GeneID:144113488"
ORIGIN      
        1 mvaahmepar aspmsarakd fsidalisrr pcdssndgsd adtpgpplll dadklcepss
       61 plllrsqrra asspetgapg lqflgrfgpg saaarlessp pssvssladd elpdapgdfa
      121 paarrsdsep ggesggstpa aprpggkqre gpskgsarve npqilprcnc delrnadahl
      181 etkdlwekfh elgtemiitk tgrrmfptlr vsfnglhpdg ryvvlldivp vdnkryryay
      241 hrsswlvagk adppsparly ahpdcpftgd qlrkqvvsfe kvkltnnemd kqghivlnsm
      301 hkyqprihlv klrpdccsnp pvitdleaeq frtyifpetv ftavtayqnq litklkidsn
      361 pfakgfrdss rltdlegesv etllrehafr hspfrafmtg dlppehqffa tagaalgrlp
      421 tgsellllan gaspwkmpam pmamadltal mnaqqqqlha lpglylgfpr tlfppfpvag
      481 aptpapvppa rspcppppts ptatgraaps asspgpplyp prtsaaaayp glkfhpyfhp
      541 rllgaaagpr egspveydaa apqv
//
LOCUS       XP_077502706             563 aa            linear   INV 25-NOV-2025
DEFINITION  T-box protein H15-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502706
VERSION     XP_077502706.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646580.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..563
                     /product="T-box protein H15-like isoform X2"
                     /calculated_mol_wt=60651
     Region          176..369
                     /region_name="T-box_TBX20-like"
                     /note="DNA-binding domain of T-box transcription factor 20
                     and related T-box proteins; cd20193"
                     /db_xref="CDD:410319"
     Site            order(203..204,237,283,286,299..300,345..348,353,356..357,
                     360..366)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410319"
     CDS             1..563
                     /gene="LOC144113488"
                     /coded_by="XM_077646580.1:136..1827"
                     /db_xref="GeneID:144113488"
ORIGIN      
        1 mvaahmepar aspmsarakd fsidalisrr pcdssndgsd adtpgpplll dadklcepss
       61 plllrsqrra asspetgapg lqflgrfgpg saaarlessp pssvssladd elpdapgdfa
      121 paarrsdsep ggesggstpa aprpggkqre gpskgsarve npqilprcnc delrnadahl
      181 etkdlwekfh elgtemiitk tgrrmfptlr vsfnglhpdg ryvvlldivp vdnkryryay
      241 hrsswlvagk adppsparly ahpdcpftgd qlrkqvvsfe kvkltnnemd kqghivlnsm
      301 hkyqprihlv klrpdccsnp pvitdleaeq frtyifpetv ftavtayqnq litklkidsn
      361 pfakgfrdss rltdlegesv etllrehafr hspfrafmtg dlppehqffa tagalgrlpt
      421 gsellllang aspwkmpamp mamadltalm naqqqqlhal pglylgfprt lfppfpvaga
      481 ptpapvppar spcpppptsp tatgraapsa sspgpplypp rtsaaaaypg lkfhpyfhpr
      541 llgaaagpre gspveydaaa pqv
//
LOCUS       XP_077502707             117 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113489 [Amblyomma americanum].
ACCESSION   XP_077502707
VERSION     XP_077502707.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646581.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..117
                     /product="uncharacterized protein LOC144113489"
                     /calculated_mol_wt=12001
     CDS             1..117
                     /gene="LOC144113489"
                     /coded_by="XM_077646581.1:88..441"
                     /db_xref="GeneID:144113489"
ORIGIN      
        1 msclrwtall allvlfvava vpvllagsda pdeeqpqqpa vaseqaagga nmdederrpe
       61 eqppeqqqeq pgetwargeg mqqpaasast aaaagvgdsc gdcsgfceak saddddk
//
LOCUS       XP_077502708             374 aa            linear   INV 25-NOV-2025
DEFINITION  per1-like protein PGAP5 [Amblyomma americanum].
ACCESSION   XP_077502708
VERSION     XP_077502708.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646582.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..374
                     /product="per1-like protein PGAP5"
                     /calculated_mol_wt=43360
     Region          64..324
                     /region_name="MPP_superfamily"
                     /note="metallophosphatase superfamily, metallophosphatase
                     domain; cl13995"
                     /db_xref="CDD:472684"
     Site            order(68,70,110,148..149,244,298)
                     /site_type="active"
                     /db_xref="CDD:277317"
     Site            order(68,70,110,148,244,298)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:277317"
     CDS             1..374
                     /gene="PGAP5"
                     /coded_by="XM_077646582.1:110..1234"
                     /db_xref="GeneID:144113491"
ORIGIN      
        1 mplrllrrrr sgflkqlifa flvliycevv iyyvvigqcs wphvekleqg rfssqeerep
       61 lkmmllsdth llgpkrghwf dklrrewqmh rtfqtaltlh rpevvaflgd vfdegqwssd
      121 eefkaymkrf wdlfyvpanv kvlvavgnhd vgfhyrmhey fvdrfeesfn tsalkltvik
      181 gnlfvtvnsm alygdncnfc arakieleqi rhklrcsegk vtrakscqkg drlgtngrpi
      241 ilmhfplyrs sdsacsepda aspeekeesf renweclsqk atvllldaln pravftghth
      301 hgcltihrks ipewtlpsis wrnkrgpsfa laaftpndma vskcyipret tviflyiiaa
      361 ivlilfsfhg rqrf
//
LOCUS       XP_077502709             465 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger CCHC domain-containing protein 8 homolog [Amblyomma
            americanum].
ACCESSION   XP_077502709
VERSION     XP_077502709.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646583.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..465
                     /product="zinc finger CCHC domain-containing protein 8
                     homolog"
                     /calculated_mol_wt=50826
     Region          152..202
                     /region_name="PSP"
                     /note="cl02643"
                     /db_xref="CDD:470641"
     CDS             1..465
                     /gene="LOC144113492"
                     /coded_by="XM_077646583.1:133..1530"
                     /db_xref="GeneID:144113492"
ORIGIN      
        1 mnetdqtqac adsvdagvnr eansteneda erppkmpkqe eedeltacng vffidknfdn
       61 fdaledppnc ttiyveesee egeekkdpas gdagpagprv ecfncggnhf lsvctkkida
      121 arvsqrrrem arerdaapkk lryfehemls qrfrpgvyse elrkalnlhp rdlppfvyrm
      181 rvigyppgwl knavvensgi kiygldeddk andtdieege ikseeksviy dtsrliripg
      241 fncpvpdgvy dmhaahgmpp lsqhqqlpsg slyvepevla aksfngrrka sasatkpstd
      301 vgevvdmnid dgaeegqvcf anentladva adsngastpt tqespapntp saasdcniat
      361 prstesdgpg qpspasveag ntsnsedlap atpkvksqnv yigtpmflgl yskiktlppr
      421 enfakdmtdy ipfenlpgtt grydklravl akmrqgkket sdket
//
LOCUS       XP_077502710             465 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger CCHC domain-containing protein 8 homolog [Amblyomma
            americanum].
ACCESSION   XP_077502710
VERSION     XP_077502710.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646584.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..465
                     /product="zinc finger CCHC domain-containing protein 8
                     homolog"
                     /calculated_mol_wt=50826
     Region          152..202
                     /region_name="PSP"
                     /note="cl02643"
                     /db_xref="CDD:470641"
     CDS             1..465
                     /gene="LOC144113492"
                     /coded_by="XM_077646584.1:122..1519"
                     /db_xref="GeneID:144113492"
ORIGIN      
        1 mnetdqtqac adsvdagvnr eansteneda erppkmpkqe eedeltacng vffidknfdn
       61 fdaledppnc ttiyveesee egeekkdpas gdagpagprv ecfncggnhf lsvctkkida
      121 arvsqrrrem arerdaapkk lryfehemls qrfrpgvyse elrkalnlhp rdlppfvyrm
      181 rvigyppgwl knavvensgi kiygldeddk andtdieege ikseeksviy dtsrliripg
      241 fncpvpdgvy dmhaahgmpp lsqhqqlpsg slyvepevla aksfngrrka sasatkpstd
      301 vgevvdmnid dgaeegqvcf anentladva adsngastpt tqespapntp saasdcniat
      361 prstesdgpg qpspasveag ntsnsedlap atpkvksqnv yigtpmflgl yskiktlppr
      421 enfakdmtdy ipfenlpgtt grydklravl akmrqgkket sdket
//
LOCUS       XP_077502712             596 aa            linear   INV 25-NOV-2025
DEFINITION  constitutive coactivator of peroxisome proliferator-activated
            receptor gamma-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502712
VERSION     XP_077502712.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646586.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..596
                     /product="constitutive coactivator of peroxisome
                     proliferator-activated receptor gamma-like isoform X1"
                     /calculated_mol_wt=66816
     Region          5..209
                     /region_name="PIN_SF"
                     /note="PIN (PilT N terminus) domain: Superfamily; cl28905"
                     /db_xref="CDD:475124"
     CDS             1..596
                     /gene="LOC144113493"
                     /coded_by="XM_077646586.1:129..1919"
                     /db_xref="GeneID:144113493"
ORIGIN      
        1 mgvrglqsfi ehsvpggctt vniinearkh srscppgtkp tivvdglsli rwiytgthdf
       61 ifagpwihls alvrsfvqaf eeqsidlvff fdgyisgskv eewrirrqrs lddiqatfgr
      121 lrsgtwtgnd andyccpngt aytlwlvvkc ftsckvfyav eecdleaary ahwhpecfal
      181 lsqdtdfvif nmgalylstl hldirtfttr ayhpvalash lglatlhlpl faclsgndtv
      241 rreqhlrefh taigfpahqr ipcrllfrki ariiqqrgwg pipdisvary tgvsldvlae
      301 gvhsydlqye acclrkpagl dhaswclalr syknatafpa llqilhrrei ylgetmeeal
      361 dgqvppahic frpvrsrmyw slfggsdtvv vtehvaypga sgiqdvdvms tsfpfngevp
      421 plyrlwsqep gminahwqlf cgclgmqlhl delmsldpad avlvstlnvm fmagvleere
      481 vsafvlqril peqdkhklls qeipdneidp hlvsistyfm iglqtitmvl sacggpfclq
      541 daapwmifcg klfhvlaqel chedsavsaa slleknpgvl elhhklwriv tgrncv
//
LOCUS       XP_077502713             524 aa            linear   INV 25-NOV-2025
DEFINITION  constitutive coactivator of peroxisome proliferator-activated
            receptor gamma-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502713
VERSION     XP_077502713.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646587.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..524
                     /product="constitutive coactivator of peroxisome
                     proliferator-activated receptor gamma-like isoform X2"
                     /calculated_mol_wt=58991
     Region          5..209
                     /region_name="PIN_SF"
                     /note="PIN (PilT N terminus) domain: Superfamily; cl28905"
                     /db_xref="CDD:475124"
     CDS             1..524
                     /gene="LOC144113493"
                     /coded_by="XM_077646587.1:123..1697"
                     /db_xref="GeneID:144113493"
ORIGIN      
        1 mgvrglqsfi ehsvpggctt vniinearkh srscppgtkp tivvdglsli rwiytgthdf
       61 ifagpwihls alvrsfvqaf eeqsidlvff fdgyisgskv eewrirrqrs lddiqatfgr
      121 lrsgtwtgnd andyccpngt aytlwlvvkc ftsckvfyav eecdleaary ahwhpecfal
      181 lsqdtdfvif nmgalylstl hldirtfttr ayhpvalash lglatlhlpl faclsgndtv
      241 rreqhlrefh taigfpahqr ipcrllfrki ariiqqrgwg pipdisvary tgvsldvlae
      301 gvhsydlqye acclrkpagl dhaswclalr syknatafpa llqilhrrei ylgetmeeal
      361 dgqvppahic frpvrsrmyw slfggsdtvv vtehvaypga sgiqdvdvms tsfpfngevp
      421 plyrlwsqep gminahwqlf cgclgmqlhl delmsldpad avlvstlnvm fmagvleere
      481 vsafvlqril peqdkhklls qeippgvlel hhklwrivtg rncv
//
LOCUS       XP_077502714             396 aa            linear   INV 25-NOV-2025
DEFINITION  NKD inhibitor of WNT signaling pathway naked cuticle isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077502714
VERSION     XP_077502714.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646588.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..396
                     /product="NKD inhibitor of WNT signaling pathway naked
                     cuticle isoform X1"
                     /calculated_mol_wt=44499
     CDS             1..396
                     /gene="nkd"
                     /coded_by="XM_077646588.1:142..1332"
                     /db_xref="GeneID:144113494"
ORIGIN      
        1 matilrkmpr ftvkfcsafs kaerslsecp lelslslcdd esckpggqfd eeqclhhgst
       61 gstekvttps ltedeerpek aeeersekee rgeglsavda scnksnqgee rqefsftlyd
      121 fnghgkvtkd diaslvrsiy dalgkkslpk sgsrtikvrl aigpdedscp aqtrplsess
      181 arssaptact lltddpppsr rapeslsvpv hrkfppqaen agnrckhssg gvppsplgsr
      241 srrvkprvhh rhellqwlke stekacyqss aaednrrsqq ataarraeap srpeavpcpy
      301 qlldrghecg gaaaspqcer rqhrrskshe eeppcrgwhh hyrhrerdle rqramrqvas
      361 wiereqlagp pplpaqrheh hhlhehvhhh yhhyvd
//
LOCUS       XP_077502715             397 aa            linear   INV 25-NOV-2025
DEFINITION  NKD inhibitor of WNT signaling pathway naked cuticle isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077502715
VERSION     XP_077502715.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646589.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..397
                     /product="NKD inhibitor of WNT signaling pathway naked
                     cuticle isoform X2"
                     /calculated_mol_wt=44628
     CDS             1..397
                     /gene="nkd"
                     /coded_by="XM_077646589.1:140..1333"
                     /db_xref="GeneID:144113494"
ORIGIN      
        1 matilrkmpr ftvkfcsafs kaerslsecp lelslslcdd esckpggqfd eeqclhhegs
       61 tgstekvttp sltedeerpe kaeeerseke ergeglsavd ascnksnqge erqefsftly
      121 dfnghgkvtk ddiaslvrsi ydalgkkslp ksgsrtikvr laigpdedsc paqtrplses
      181 sarssaptac tlltddppps rrapeslsvp vhrkfppqae nagnrckhss ggvppsplgs
      241 rsrrvkprvh hrhellqwlk estekacyqs saaednrrsq qataarraea psrpeavpcp
      301 yqlldrghec ggaaaspqce rrqhrrsksh eeeppcrgwh hhyrhrerdl erqramrqva
      361 swiereqlag ppplpaqrhe hhhlhehvhh hyhhyvd
//
LOCUS       XP_077502716             242 aa            linear   INV 25-NOV-2025
DEFINITION  transmembrane protein 70 homolog, mitochondrial [Amblyomma
            americanum].
ACCESSION   XP_077502716
VERSION     XP_077502716.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646590.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..242
                     /product="transmembrane protein 70 homolog, mitochondrial"
                     /calculated_mol_wt=26735
     Region          65..208
                     /region_name="TMEM70"
                     /note="TMEM70/TMEM186/TMEM223 protein family; pfam06979"
                     /db_xref="CDD:462058"
     CDS             1..242
                     /gene="LOC144113495"
                     /coded_by="XM_077646590.1:149..877"
                     /db_xref="GeneID:144113495"
ORIGIN      
        1 mtilrtlhtl ssknfltgaa ilhaqrcdrl hgkyaeaffa nglqrrgist sspmkhshdh
       61 eksssktlvy lgsirstvkm vkslsltssf lgilaqpill qklsgasvga tvivvscasf
      121 fifvtplmlh yitrryvtel tydpdtkefn attlsllnrk keisfiaddv evptvpgpft
      181 tllakgqplf vevqnfqnad alehllgydk pidweikpph peeteqvltk rkssqgscdk
      241 ls
//
LOCUS       XP_077502717             946 aa            linear   INV 25-NOV-2025
DEFINITION  GPI ethanolamine phosphate transferase 1-like [Amblyomma
            americanum].
ACCESSION   XP_077502717
VERSION     XP_077502717.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646591.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..946
                     /product="GPI ethanolamine phosphate transferase 1-like"
                     /calculated_mol_wt=107984
     Region          43..348
                     /region_name="GPI_EPT_1"
                     /note="GPI ethanolamine phosphate transferase 1; PIG-N;
                     cd16020"
                     /db_xref="CDD:293744"
     Site            order(55..56,97,217,265..266)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:293744"
     Site            order(55,97,217,266)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:293744"
     Region          433..898
                     /region_name="PigN"
                     /note="Phosphatidylinositolglycan class N (PIG-N);
                     pfam04987"
                     /db_xref="CDD:461508"
     CDS             1..946
                     /gene="LOC144113496"
                     /coded_by="XM_077646591.1:225..3065"
                     /db_xref="GeneID:144113496"
ORIGIN      
        1 mllsvfagii glavhvvflf sifdiyfksp iihglpayev plpspasrlv livadglrad
       61 kvfelqkngt srapylrsvi tekgswgvsh trvptesrpg hvaliagfye dvsavtkgwk
      121 hnpvefdsvf nrsrhvwawg aadivhlftk gdyvkhihah tydtneidfa dedasrldlw
      181 vfgklqeflr saednqtlne mlkrdktvff lhllgldtig hgytpeskey cenialvdrn
      241 vkdvvklfee fyqedgktay vftsdhgmtd wgnhgagsss etetpliawg agialprlsg
      301 rdgyydgyse awnlslyrrv dinqadlapl maillgmaip vnslgllpve ylntdwhfra
      361 valltnarqi laqfkqqkhi ieettfslff rqfrdlmpkr eveltemiet llkqsrykea
      421 vqviekfiel alkglvyyqk ydrltlsiav algfigwmaf villvlrnyt gimckslesq
      481 snkrnqewqt kvkiistsil vlflstvlly vqnapfmyyf yflipivlwt mvfyeldvyy
      541 eakaylrrfe vkmwfvaltf iailamelvv itffyrgvms lglliigcwp fstslskkts
      601 ftwligclvl gifpllpvig kqhnytmvtl sgwvsilvfs ycarrpelgl vrnsrqipke
      661 pqrsviltav qivliwvavt ivrstadsie hkeglplfnq ilswlllifs pvlclfstts
      721 llnrlqnltl sllvpfilmc ifyeslflmv lcfvmflwic iehqlsgstl rlqdmtfesq
      781 dvslqakavt yhiriddirk ayffiffmlv afygtgnias lnsfsissfy cfitvfrpft
      841 maaillikvl ipllivscaf rallqsikvs ntalfllvvv lsdftalhff flikdsgswl
      901 digmsishyl lamgmfifta vfhglawfmt tfslecsghe fkrhll
//
LOCUS       XP_077502718             310 aa            linear   INV 25-NOV-2025
DEFINITION  calbindin 53E isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502718
VERSION     XP_077502718.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646592.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..310
                     /product="calbindin 53E isoform X1"
                     /calculated_mol_wt=35555
     Region          35..300
                     /region_name="EFh_HEF_CBN"
                     /note="EF-hand, calcium binding motif, found in Drosophila
                     melanogaster calbindin-32 (CBN) and similar proteins;
                     cd16179"
                     /db_xref="CDD:320079"
     Region          35..63
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320079"
     Site            order(43,45,47,54,93,95,97,104,139,141,143,150,184,186,
                     188,195,237,239,241,248)
                     /site_type="other"
                     /note="putative Ca binding site [ion binding]"
                     /db_xref="CDD:320079"
     Region          84..113
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320079"
     Region          130..159
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320079"
     Region          175..204
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320079"
     Region          228..257
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320079"
     Region          272..300
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320079"
     CDS             1..310
                     /gene="Cbp53E"
                     /coded_by="XM_077646592.1:268..1200"
                     /db_xref="GeneID:144113498"
ORIGIN      
        1 maestkagnk ssanfmrqfr dkktrelknl tasqfmdvws hydkdgngfi egreldnflr
       61 efvssvslsd vgpevvsesm leelkecfme ayddnkdgki eirelaqllp leesflllfr
      121 fdnplessve fmkiwreydt dgsgyieade lknflkdllr eakkdsieed klieytdtll
      181 qifdsnkdgk lqlsemakll pvkenflcrs afkltdatag askltredie rvfalydrdk
      241 ngtieneelk gflkdllelv kkdydaqdle efqeailrgc dfnkdgkisk keltmvllal
      301 akhsadedgk
//
LOCUS       XP_077502719             304 aa            linear   INV 25-NOV-2025
DEFINITION  calbindin 53E isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502719
VERSION     XP_077502719.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646593.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..304
                     /product="calbindin 53E isoform X2"
                     /calculated_mol_wt=34983
     Region          35..294
                     /region_name="EFh_HEF_CBN"
                     /note="EF-hand, calcium binding motif, found in Drosophila
                     melanogaster calbindin-32 (CBN) and similar proteins;
                     cd16179"
                     /db_xref="CDD:320079"
     Region          35..63
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320079"
     Site            order(43,45,47,54,93,95,97,104,139,141,143,150,184,186,
                     188,195,231,233,235,242)
                     /site_type="other"
                     /note="putative Ca binding site [ion binding]"
                     /db_xref="CDD:320079"
     Region          84..113
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320079"
     Region          130..159
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320079"
     Region          175..204
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320079"
     Region          222..251
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320079"
     Region          266..294
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320079"
     CDS             1..304
                     /gene="Cbp53E"
                     /coded_by="XM_077646593.1:266..1180"
                     /db_xref="GeneID:144113498"
ORIGIN      
        1 maestkagnk ssanfmrqfr dkktrelknl tasqfmdvws hydkdgngfi egreldnflr
       61 efvssvslsd vgpevvsesm leelkecfme ayddnkdgki eirelaqllp leesflllfr
      121 fdnplessve fmkiwreydt dgsgyieade lknflkdllr eakkdsieed klieytdtll
      181 qifdsnkdgk lqlsemakll pvkenflcrs afkgaskltr ediervfaly drdkngtien
      241 eelkgflkdl lelvkkdyda qdleefqeai lrgcdfnkdg kiskkeltmv llalakhsad
      301 edgk
//
LOCUS       XP_077502720             613 aa            linear   INV 25-NOV-2025
DEFINITION  glycosylphosphatidylinositol anchor attachment 1 [Amblyomma
            americanum].
ACCESSION   XP_077502720
VERSION     XP_077502720.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646594.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..613
                     /product="glycosylphosphatidylinositol anchor attachment
                     1"
                     /calculated_mol_wt=67685
     Region          121..611
                     /region_name="Gaa1"
                     /note="Gaa1-like, GPI transamidase component; pfam04114"
                     /db_xref="CDD:461181"
     CDS             1..613
                     /gene="GAA1"
                     /coded_by="XM_077646594.1:357..2198"
                     /db_xref="GeneID:144113501"
ORIGIN      
        1 mgvlmntsqn skvvllvqkh cnvictlayv agliafavla sdecnnktyf senallpglv
       61 nrrfdlgrfa kdaleslkee aknhaslplp wllgqfrqlg ldvfrhnfsl hyplgskpth
      121 hgenvyailr aprgagteav vlsspyrmed nlhgstlpgi almvalakyf raqgswakdi
      181 iflitehelv gfqawldayh dvhtapgvid pgmlqarsgp vqaainlelh ssrikrldlk
      241 lvglngqlpn ldlfnlvtel clresvhttf hgqvspydlp pfedwvqsfk tmtammaaqa
      301 tgqpngghgl fhryaiqalt leghadgvas vsvgfnevgr vlegvfssln nllerfhqsf
      361 ffyllpstrr yisiglyspa faliglpall kagvlflsls gdaksadaas tketaspwsa
      421 lpcmtvshtv gfllmvapsy fdhfghhfhl ssqesvyfgy vaalivslll plvvgprkek
      481 ersahrcavl lafstvvfsl alvnisfata vtvitipvlv tagstehwva vlfhrllqll
      541 ihpfvfsfli ifvqamsfdg wdpqfirnnl sysflghkkf vvfsvedwll ygnwtfvitc
      601 lglipvwlqm wmv
//
LOCUS       XP_077502722             321 aa            linear   INV 25-NOV-2025
DEFINITION  partitioning defective protein 6 [Amblyomma americanum].
ACCESSION   XP_077502722
VERSION     XP_077502722.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646596.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..321
                     /product="partitioning defective protein 6"
                     /calculated_mol_wt=35356
     Region          17..97
                     /region_name="PB1_Par6"
                     /note="The PB1 domain is an essential part of Par6 protein
                     which in complex with Par3 and aPKC proteins is crucial
                     for establishment of apical-basal polarity of animal
                     cells. The PB1 domain is a modular domain mediating
                     specific protein-protein interactions...; cd06403"
                     /db_xref="CDD:99724"
     Site            order(18,20,27..29,31,46,50,91)
                     /site_type="other"
                     /note="PB1 interaction surface [polypeptide binding]"
                     /db_xref="CDD:99724"
     Region          160..252
                     /region_name="PDZ_Par6-like"
                     /note="PDZ domain of partitioning defective 6 (Par6),
                     Drosophila Rho GTPase-activating protein 100F
                     (RhoGAP100F), and related domains; cd06718"
                     /db_xref="CDD:467202"
     Site            order(172..178,199,202,232,236,239..240)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467202"
     CDS             1..321
                     /gene="par-6"
                     /coded_by="XM_077646596.1:96..1061"
                     /db_xref="GeneID:144113502"
ORIGIN      
        1 mskplknlhh lqngsilevk skfdaefrrf sldkaelsry eefcrliekf hnigevpfsl
       61 cytdpihgdl lpinndenfa ravqsarpll rlliqrkgds reelngfgap gasrkkrflg
      121 qyllpvgvrt arpalsislp edfrqvssii dvdvvpdtcr rvrlvkhgsd kplgfyirdg
      181 tsvrvtphgl drlpgifisr lvpgglaegt gllavndevl evngievsgk tldqvtdmmv
      241 anssnliiti rpanqcsvgt lprrgsfgrs sqmshgshns npsvvsdddr ldedeirdht
      301 agfegldetp prsthdgvit l
//
LOCUS       XP_077502723             270 aa            linear   INV 25-NOV-2025
DEFINITION  twisted gastrulation protein homolog 1-A-like [Amblyomma
            americanum].
ACCESSION   XP_077502723
VERSION     XP_077502723.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646597.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..270
                     /product="twisted gastrulation protein homolog 1-A-like"
                     /calculated_mol_wt=29928
     Region          80..210
                     /region_name="Tsg"
                     /note="Twisted gastrulation (Tsg) protein conserved
                     region; pfam04668"
                     /db_xref="CDD:461385"
     CDS             1..270
                     /gene="LOC144113503"
                     /coded_by="XM_077646597.1:1..813"
                     /db_xref="GeneID:144113503"
ORIGIN      
        1 mlkqvllpla vavacalsls lacneaicas ivskcmltqs ckcdklnvtc srdcylcldy
       61 lypeccscve mcprqnetis lshksniedl pdpthglfal lteeadrslr wrsytfpvhv
      121 alyspvhdkd iklkavvssg naseaveeed vqvnctvafm sqcmslnkcr esclsmgass
      181 yrwfhdgccq cvgsmcinyg inesrclqcp yskelmdldd eqdeevpied pdrhtyqhvd
      241 ysdnaysake kaedrnnavd tsaahnanst
//
LOCUS       XP_077502724             532 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113504 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502724
VERSION     XP_077502724.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646598.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (DAPXPI010028372.1) annotated
            using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 186 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..532
                     /product="uncharacterized protein LOC144113504 isoform X1"
                     /calculated_mol_wt=55224
     Region          58..165
                     /region_name="DUF4745"
                     /note="Domain of unknown function (DUF4745); pfam15923"
                     /db_xref="CDD:374226"
     CDS             1..532
                     /gene="LOC144113504"
                     /coded_by="XM_077646598.1:249..1847"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 186 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144113504"
ORIGIN      
        1 manlrrhfnf gshksgsint fglshghqss segiafpdha fsrsafmnev csfldhcsay
       61 iqalnsfcss ssvlakslvr vfhnvpvyre vaalflgawe dlgrvtaaas aavktetlmt
      121 lqdvlnkleg eapgdesaal tesiqavgsc lgsylelqvq fsysawkals kisksfpqdr
      181 nssksaaaks saelaatikk hfasllhklg astkqgslas givtdsqqsq fyvegtvpap
      241 sslapapegp aqpggknlaw thtseelmgs gngsqpavqp gqsesgllgv ldtggdledv
      301 inllsaaphp sptagpkgfl reaalalwpp paatapgncs gkrtwprpts gahglsafgm
      361 dpfvwsslgq fdqralcsgl pssqsdcmsg gfwssgerqg wsslvsgeng sssdesssnn
      421 gqdsdtflmg lnlagsdlva svrqrrhsss dgppegmhna lesssntdsv lletravkts
      481 twplkqsssf pvhlplshsv gdaasdggcl ddaagsgfsg svnsyflfgh gs
//
LOCUS       XP_077502725             532 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113504 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502725
VERSION     XP_077502725.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646599.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (DAPXPI010028372.1) annotated
            using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 186 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..532
                     /product="uncharacterized protein LOC144113504 isoform X1"
                     /calculated_mol_wt=55224
     Region          58..165
                     /region_name="DUF4745"
                     /note="Domain of unknown function (DUF4745); pfam15923"
                     /db_xref="CDD:374226"
     CDS             1..532
                     /gene="LOC144113504"
                     /coded_by="XM_077646599.1:194..1792"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 186 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144113504"
ORIGIN      
        1 manlrrhfnf gshksgsint fglshghqss segiafpdha fsrsafmnev csfldhcsay
       61 iqalnsfcss ssvlakslvr vfhnvpvyre vaalflgawe dlgrvtaaas aavktetlmt
      121 lqdvlnkleg eapgdesaal tesiqavgsc lgsylelqvq fsysawkals kisksfpqdr
      181 nssksaaaks saelaatikk hfasllhklg astkqgslas givtdsqqsq fyvegtvpap
      241 sslapapegp aqpggknlaw thtseelmgs gngsqpavqp gqsesgllgv ldtggdledv
      301 inllsaaphp sptagpkgfl reaalalwpp paatapgncs gkrtwprpts gahglsafgm
      361 dpfvwsslgq fdqralcsgl pssqsdcmsg gfwssgerqg wsslvsgeng sssdesssnn
      421 gqdsdtflmg lnlagsdlva svrqrrhsss dgppegmhna lesssntdsv lletravkts
      481 twplkqsssf pvhlplshsv gdaasdggcl ddaagsgfsg svnsyflfgh gs
//
LOCUS       XP_077502726             532 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113504 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502726
VERSION     XP_077502726.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646600.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (DAPXPI010028372.1) annotated
            using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 186 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..532
                     /product="uncharacterized protein LOC144113504 isoform X1"
                     /calculated_mol_wt=55224
     Region          58..165
                     /region_name="DUF4745"
                     /note="Domain of unknown function (DUF4745); pfam15923"
                     /db_xref="CDD:374226"
     CDS             1..532
                     /gene="LOC144113504"
                     /coded_by="XM_077646600.1:289..1887"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 186 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144113504"
ORIGIN      
        1 manlrrhfnf gshksgsint fglshghqss segiafpdha fsrsafmnev csfldhcsay
       61 iqalnsfcss ssvlakslvr vfhnvpvyre vaalflgawe dlgrvtaaas aavktetlmt
      121 lqdvlnkleg eapgdesaal tesiqavgsc lgsylelqvq fsysawkals kisksfpqdr
      181 nssksaaaks saelaatikk hfasllhklg astkqgslas givtdsqqsq fyvegtvpap
      241 sslapapegp aqpggknlaw thtseelmgs gngsqpavqp gqsesgllgv ldtggdledv
      301 inllsaaphp sptagpkgfl reaalalwpp paatapgncs gkrtwprpts gahglsafgm
      361 dpfvwsslgq fdqralcsgl pssqsdcmsg gfwssgerqg wsslvsgeng sssdesssnn
      421 gqdsdtflmg lnlagsdlva svrqrrhsss dgppegmhna lesssntdsv lletravkts
      481 twplkqsssf pvhlplshsv gdaasdggcl ddaagsgfsg svnsyflfgh gs
//
LOCUS       XP_077502727             414 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113504 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502727
VERSION     XP_077502727.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646601.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (DAPXPI010028372.1) annotated
            using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 186 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..414
                     /product="uncharacterized protein LOC144113504 isoform X2"
                     /calculated_mol_wt=42460
     CDS             1..414
                     /gene="LOC144113504"
                     /coded_by="XM_077646601.1:341..1585"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 186 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144113504"
ORIGIN      
        1 mtlqdvlnkl egeapgdesa altesiqavg sclgsylelq vqfsysawka lskisksfpq
       61 drnssksaaa kssaelaati kkhfasllhk lgastkqgsl asgivtdsqq sqfyvegtvp
      121 apsslapape gpaqpggknl awthtseelm gsgngsqpav qpgqsesgll gvldtggdle
      181 dvinllsaap hpsptagpkg flreaalalw pppaatapgn csgkrtwprp tsgahglsaf
      241 gmdpfvwssl gqfdqralcs glpssqsdcm sggfwssger qgwsslvsge ngsssdesss
      301 nngqdsdtfl mglnlagsdl vasvrqrrhs ssdgppegmh nalesssntd svlletravk
      361 tstwplkqss sfpvhlplsh svgdaasdgg clddaagsgf sgsvnsyflf ghgs
//
LOCUS       XP_077502728             578 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113505 [Amblyomma americanum].
ACCESSION   XP_077502728
VERSION     XP_077502728.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646602.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..578
                     /product="uncharacterized protein LOC144113505"
                     /calculated_mol_wt=64762
     CDS             1..578
                     /gene="LOC144113505"
                     /coded_by="XM_077646602.1:754..2490"
                     /db_xref="GeneID:144113505"
ORIGIN      
        1 meartdgyty ngkkeepsap titstpadiv ssssplssge qglasmaeps tlngsasdnh
       61 gedglseepi rkrrrssnds tngtatptgk grssrgdkvr apdsdttcpr krggnsssdt
      121 esgpseqemd gdpatqdsyg nghspkvppl kivlpssgan nlegsepags tnskerkvss
      181 skpalpyvvn aaeslgegsp geqaveeker rreeercqrv trsshraals hedevedape
      241 vhprkrklra reeqqqplqq qqqqqqqqqq qqqqqqqqqq qqqqqhqpll qqqqqqqqhq
      301 pllqqqqqqq plqqqqqsgl apslgppplv eemsqpvtng fqmfldirkq vekrrqamlv
      361 vhpkppqgfk dyllnrgsyv legnpssrls vpmiplpqsl sgspmrdlfl eqereryklr
      421 lqhlierekl vlsaeqeilr vhgraaraiq nqavplsacs ilrdeeiynv lkpeqicvks
      481 pesiadkkdd kdknvrsryn grlflswlqd vedkwektke kmllrqhnea eslhavqkld
      541 wewklkelgl cevkatpvid dicvpsvhvs defdvlpa
//
LOCUS       XP_077502729             578 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113505 [Amblyomma americanum].
ACCESSION   XP_077502729
VERSION     XP_077502729.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646603.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..578
                     /product="uncharacterized protein LOC144113505"
                     /calculated_mol_wt=64762
     CDS             1..578
                     /gene="LOC144113505"
                     /coded_by="XM_077646603.1:586..2322"
                     /db_xref="GeneID:144113505"
ORIGIN      
        1 meartdgyty ngkkeepsap titstpadiv ssssplssge qglasmaeps tlngsasdnh
       61 gedglseepi rkrrrssnds tngtatptgk grssrgdkvr apdsdttcpr krggnsssdt
      121 esgpseqemd gdpatqdsyg nghspkvppl kivlpssgan nlegsepags tnskerkvss
      181 skpalpyvvn aaeslgegsp geqaveeker rreeercqrv trsshraals hedevedape
      241 vhprkrklra reeqqqplqq qqqqqqqqqq qqqqqqqqqq qqqqqhqpll qqqqqqqqhq
      301 pllqqqqqqq plqqqqqsgl apslgppplv eemsqpvtng fqmfldirkq vekrrqamlv
      361 vhpkppqgfk dyllnrgsyv legnpssrls vpmiplpqsl sgspmrdlfl eqereryklr
      421 lqhlierekl vlsaeqeilr vhgraaraiq nqavplsacs ilrdeeiynv lkpeqicvks
      481 pesiadkkdd kdknvrsryn grlflswlqd vedkwektke kmllrqhnea eslhavqkld
      541 wewklkelgl cevkatpvid dicvpsvhvs defdvlpa
//
LOCUS       XP_077502730             578 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113505 [Amblyomma americanum].
ACCESSION   XP_077502730
VERSION     XP_077502730.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646604.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..578
                     /product="uncharacterized protein LOC144113505"
                     /calculated_mol_wt=64762
     CDS             1..578
                     /gene="LOC144113505"
                     /coded_by="XM_077646604.1:469..2205"
                     /db_xref="GeneID:144113505"
ORIGIN      
        1 meartdgyty ngkkeepsap titstpadiv ssssplssge qglasmaeps tlngsasdnh
       61 gedglseepi rkrrrssnds tngtatptgk grssrgdkvr apdsdttcpr krggnsssdt
      121 esgpseqemd gdpatqdsyg nghspkvppl kivlpssgan nlegsepags tnskerkvss
      181 skpalpyvvn aaeslgegsp geqaveeker rreeercqrv trsshraals hedevedape
      241 vhprkrklra reeqqqplqq qqqqqqqqqq qqqqqqqqqq qqqqqhqpll qqqqqqqqhq
      301 pllqqqqqqq plqqqqqsgl apslgppplv eemsqpvtng fqmfldirkq vekrrqamlv
      361 vhpkppqgfk dyllnrgsyv legnpssrls vpmiplpqsl sgspmrdlfl eqereryklr
      421 lqhlierekl vlsaeqeilr vhgraaraiq nqavplsacs ilrdeeiynv lkpeqicvks
      481 pesiadkkdd kdknvrsryn grlflswlqd vedkwektke kmllrqhnea eslhavqkld
      541 wewklkelgl cevkatpvid dicvpsvhvs defdvlpa
//
LOCUS       XP_077502731             578 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113505 [Amblyomma americanum].
ACCESSION   XP_077502731
VERSION     XP_077502731.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646605.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..578
                     /product="uncharacterized protein LOC144113505"
                     /calculated_mol_wt=64762
     CDS             1..578
                     /gene="LOC144113505"
                     /coded_by="XM_077646605.1:533..2269"
                     /db_xref="GeneID:144113505"
ORIGIN      
        1 meartdgyty ngkkeepsap titstpadiv ssssplssge qglasmaeps tlngsasdnh
       61 gedglseepi rkrrrssnds tngtatptgk grssrgdkvr apdsdttcpr krggnsssdt
      121 esgpseqemd gdpatqdsyg nghspkvppl kivlpssgan nlegsepags tnskerkvss
      181 skpalpyvvn aaeslgegsp geqaveeker rreeercqrv trsshraals hedevedape
      241 vhprkrklra reeqqqplqq qqqqqqqqqq qqqqqqqqqq qqqqqhqpll qqqqqqqqhq
      301 pllqqqqqqq plqqqqqsgl apslgppplv eemsqpvtng fqmfldirkq vekrrqamlv
      361 vhpkppqgfk dyllnrgsyv legnpssrls vpmiplpqsl sgspmrdlfl eqereryklr
      421 lqhlierekl vlsaeqeilr vhgraaraiq nqavplsacs ilrdeeiynv lkpeqicvks
      481 pesiadkkdd kdknvrsryn grlflswlqd vedkwektke kmllrqhnea eslhavqkld
      541 wewklkelgl cevkatpvid dicvpsvhvs defdvlpa
//
LOCUS       XP_077502732             578 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113505 [Amblyomma americanum].
ACCESSION   XP_077502732
VERSION     XP_077502732.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646606.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..578
                     /product="uncharacterized protein LOC144113505"
                     /calculated_mol_wt=64762
     CDS             1..578
                     /gene="LOC144113505"
                     /coded_by="XM_077646606.1:402..2138"
                     /db_xref="GeneID:144113505"
ORIGIN      
        1 meartdgyty ngkkeepsap titstpadiv ssssplssge qglasmaeps tlngsasdnh
       61 gedglseepi rkrrrssnds tngtatptgk grssrgdkvr apdsdttcpr krggnsssdt
      121 esgpseqemd gdpatqdsyg nghspkvppl kivlpssgan nlegsepags tnskerkvss
      181 skpalpyvvn aaeslgegsp geqaveeker rreeercqrv trsshraals hedevedape
      241 vhprkrklra reeqqqplqq qqqqqqqqqq qqqqqqqqqq qqqqqhqpll qqqqqqqqhq
      301 pllqqqqqqq plqqqqqsgl apslgppplv eemsqpvtng fqmfldirkq vekrrqamlv
      361 vhpkppqgfk dyllnrgsyv legnpssrls vpmiplpqsl sgspmrdlfl eqereryklr
      421 lqhlierekl vlsaeqeilr vhgraaraiq nqavplsacs ilrdeeiynv lkpeqicvks
      481 pesiadkkdd kdknvrsryn grlflswlqd vedkwektke kmllrqhnea eslhavqkld
      541 wewklkelgl cevkatpvid dicvpsvhvs defdvlpa
//
LOCUS       XP_077502733             578 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113505 [Amblyomma americanum].
ACCESSION   XP_077502733
VERSION     XP_077502733.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646607.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..578
                     /product="uncharacterized protein LOC144113505"
                     /calculated_mol_wt=64762
     CDS             1..578
                     /gene="LOC144113505"
                     /coded_by="XM_077646607.1:393..2129"
                     /db_xref="GeneID:144113505"
ORIGIN      
        1 meartdgyty ngkkeepsap titstpadiv ssssplssge qglasmaeps tlngsasdnh
       61 gedglseepi rkrrrssnds tngtatptgk grssrgdkvr apdsdttcpr krggnsssdt
      121 esgpseqemd gdpatqdsyg nghspkvppl kivlpssgan nlegsepags tnskerkvss
      181 skpalpyvvn aaeslgegsp geqaveeker rreeercqrv trsshraals hedevedape
      241 vhprkrklra reeqqqplqq qqqqqqqqqq qqqqqqqqqq qqqqqhqpll qqqqqqqqhq
      301 pllqqqqqqq plqqqqqsgl apslgppplv eemsqpvtng fqmfldirkq vekrrqamlv
      361 vhpkppqgfk dyllnrgsyv legnpssrls vpmiplpqsl sgspmrdlfl eqereryklr
      421 lqhlierekl vlsaeqeilr vhgraaraiq nqavplsacs ilrdeeiynv lkpeqicvks
      481 pesiadkkdd kdknvrsryn grlflswlqd vedkwektke kmllrqhnea eslhavqkld
      541 wewklkelgl cevkatpvid dicvpsvhvs defdvlpa
//
LOCUS       XP_077502735             796 aa            linear   INV 25-NOV-2025
DEFINITION  SPG7 matrix AAA peptidase subunit, paraplegin isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502735
VERSION     XP_077502735.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646609.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..796
                     /product="SPG7 matrix AAA peptidase subunit, paraplegin
                     isoform X1"
                     /calculated_mol_wt=88216
     Region          143..747
                     /region_name="HflB"
                     /note="ATP-dependent Zn proteases [Posttranslational
                     modification, protein turnover, chaperones]; COG0465"
                     /db_xref="CDD:440233"
     CDS             1..796
                     /gene="Spg7"
                     /coded_by="XM_077646609.1:139..2529"
                     /db_xref="GeneID:144113508"
ORIGIN      
        1 mphgigimmi gsaskvsyhq qyqvmryavn fisthlrass frglqeartv rpffgwsgrp
       61 atkrlcsqid selkavrgll krsgvlagdq ctfssllgst ralhttsfrg qdhngknnkd
      121 kddeeskvsl lakaaiwmmt ayivitlmsl lfptsnqpdv lryvswnefy hqmlakgeve
      181 eiivrpeldl vtiylhdnai ikgrkaehkt fhmnivdveh feeklrmaek slgihadagv
      241 plvyernqes twlllaslia valmillmfr sgtiktpqam dffsqmgrar ftivdpltgs
      301 gkgvrfkdva glqeakqeim efidylkrpe rytslgakip kgvlllgppg cgktmlakav
      361 ateasvpfla magsefiemi gglgaarvrd lfkearkrap civyideida igrkrsnvga
      421 egstgeeeqt lnqllvemdg matkegvill astnrsevld kallrpgrfd rhilidlptl
      481 aerkeifeqh lkvinldnpp shysqrlaql tpgfigadia nvcneaalha arnkekvvss
      541 gnleyaverv vggtekrsqv msltekevva fhecghalvg wllehtdalm kvsivprtsn
      601 algfaqylpt dqklysyeql fqkmcmalgg rvaesltfnr istgaeddlk kvrkmayami
      661 hqygmdpvig plsfpaeeeg qivvgrkpys rrlantideq arlvvvrayk etervlqqns
      721 dklkllagel lkrevlnyad iealigpppf gkkqlievld lgpdssdkkn skpsseregp
      781 hgkpqqnlsg atgtst
//
LOCUS       XP_077502736             797 aa            linear   INV 25-NOV-2025
DEFINITION  SPG7 matrix AAA peptidase subunit, paraplegin isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502736
VERSION     XP_077502736.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646610.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..797
                     /product="SPG7 matrix AAA peptidase subunit, paraplegin
                     isoform X2"
                     /calculated_mol_wt=88289
     Region          143..748
                     /region_name="HflB"
                     /note="ATP-dependent Zn proteases [Posttranslational
                     modification, protein turnover, chaperones]; COG0465"
                     /db_xref="CDD:440233"
     CDS             1..797
                     /gene="Spg7"
                     /coded_by="XM_077646610.1:158..2551"
                     /db_xref="GeneID:144113508"
ORIGIN      
        1 mphgigimmi gsaskvsyhq qyqvmryavn fisthlrass frglqeartv rpffgwsgrp
       61 atkrlcsqid selkavrgll krsgvlagdq ctfssllgst ralhttsfrg qdhngknnkd
      121 kddeeskvsl lakaaiwmmt ayivitlmsl lfptsnqpdv lryvswnefy hqmlakgeve
      181 eiivrpeldl vtiylhdnai ikgrkaehkt fhmnivdveh feeklrmaek slgihadagv
      241 plvyernqes twlllaslia valmillmfr sgtiktpqam dffsqmgrar ftivdpltgs
      301 gkgvrfkdva glqeakqeim efidylkrpe rytslgakip kgvlllgppg cgktmlakav
      361 ateasvpfla magsefiemi gglgaarvrd lfkearkrap civyideida igrkrsnvga
      421 egstgeeeqt lnqllvemdg matkegvill astnrsevld kallrpgrfd rhilidlptl
      481 aerkeifeqh lkvinldnpp shysqrlaql tpgfsvgadi anvcneaalh aarnkekvvs
      541 sgnleyaver vvggtekrsq vmsltekevv afhecghalv gwllehtdal mkvsivprts
      601 nalgfaqylp tdqklysyeq lfqkmcmalg grvaesltfn ristgaeddl kkvrkmayam
      661 ihqygmdpvi gplsfpaeee gqivvgrkpy srrlantide qarlvvvray ketervlqqn
      721 sdklkllage llkrevlnya diealigppp fgkkqlievl dlgpdssdkk nskpssereg
      781 phgkpqqnls gatgtst
//
LOCUS       XP_077502737             796 aa            linear   INV 25-NOV-2025
DEFINITION  SPG7 matrix AAA peptidase subunit, paraplegin isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502737
VERSION     XP_077502737.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646611.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..796
                     /product="SPG7 matrix AAA peptidase subunit, paraplegin
                     isoform X3"
                     /calculated_mol_wt=88190
     Region          143..747
                     /region_name="HflB"
                     /note="ATP-dependent Zn proteases [Posttranslational
                     modification, protein turnover, chaperones]; COG0465"
                     /db_xref="CDD:440233"
     CDS             1..796
                     /gene="Spg7"
                     /coded_by="XM_077646611.1:158..2548"
                     /db_xref="GeneID:144113508"
ORIGIN      
        1 mphgigimmi gsaskvsyhq qyqvmryavn fisthlrass frglqeartv rpffgwsgrp
       61 atkrlcsqid selkavrgll krsgvlagdq ctfssllgst ralhttsfrg qdhngknnkd
      121 kddeeskvsl lakaaiwmmt ayivitlmsl lfptsnqpdv lryvswnefy hqmlakgeve
      181 eiivrpeldl vtiylhdnai ikgrkaehkt fhmnivdveh feeklrmaek slgihadagv
      241 plvyernqes twlllaslia valmillmfr sgtiktpqam dffsqmgrar ftivdpltgs
      301 gkgvrfkdva glqeakqeim efidylkrpe rytslgakip kgvlllgppg cgktmlakav
      361 ateasvpfla magsefiemi gglgaarvrd lfkearkrap civyideida igrkrsnvga
      421 egstgeeeqt lnqllvemdg matkegvill astnrsevld kallrpgrfd rhilidlptl
      481 aerkeifeqh lkvinldnpp shysqrlaql tpgfsgadia nvcneaalha arnkekvvss
      541 gnleyaverv vggtekrsqv msltekevva fhecghalvg wllehtdalm kvsivprtsn
      601 algfaqylpt dqklysyeql fqkmcmalgg rvaesltfnr istgaeddlk kvrkmayami
      661 hqygmdpvig plsfpaeeeg qivvgrkpys rrlantideq arlvvvrayk etervlqqns
      721 dklkllagel lkrevlnyad iealigpppf gkkqlievld lgpdssdkkn skpsseregp
      781 hgkpqqnlsg atgtst
//
LOCUS       XP_077502738             306 aa            linear   INV 25-NOV-2025
DEFINITION  hemK methyltransferase 1 [Amblyomma americanum].
ACCESSION   XP_077502738
VERSION     XP_077502738.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646612.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..306
                     /product="hemK methyltransferase 1"
                     /calculated_mol_wt=33848
     Region          7..304
                     /region_name="HemK"
                     /note="Methylase of polypeptide chain release factors
                     [Translation, ribosomal structure and biogenesis];
                     COG2890"
                     /db_xref="CDD:442135"
     CDS             1..306
                     /gene="HemK1"
                     /coded_by="XM_077646612.1:172..1092"
                     /db_xref="GeneID:144113509"
ORIGIN      
        1 mrqfcssaaq ftsywmrifa ragidageak aslncflssv lgrrraleiq ahpdsaipil
       61 pshlnklnhy crrraqrlpv qyilgkwsfh gialkmcppv fiprpetegl vdivlshinr
      121 sendvrhvld igcgtgaicl glasctskvh ytaidrspvs vkltqenssq lglservscy
      181 laeatpdglk csepnlnmsl ydaivsnppy iaiqdsstie peilkhedht alfagpdgld
      241 vvrsilqlsr rllrvgghlf levgllqppl iramlsaeeh ghlfelvavh pdftsrprfv
      301 eikriq
//
LOCUS       XP_077502739             559 aa            linear   INV 25-NOV-2025
DEFINITION  glucose-6-phosphate isomerase [Amblyomma americanum].
ACCESSION   XP_077502739
VERSION     XP_077502739.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646613.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..559
                     /product="glucose-6-phosphate isomerase"
                     /calculated_mol_wt=62634
     Region          14..559
                     /region_name="pgi"
                     /note="glucose-6-phosphate isomerase; Reviewed; PRK00179"
                     /db_xref="CDD:234679"
     CDS             1..559
                     /gene="Pgi"
                     /coded_by="XM_077646613.1:128..1807"
                     /db_xref="GeneID:144113510"
ORIGIN      
        1 mttfvqdhfl vedpafkklq eyynsngkkl nmpelfkkdp erfkkcsikl dtpdsgvllf
       61 dysknlinee vlgklfdlvk srkvtlarda mfrgekinft egravlhval rnrsntpilv
      121 dgkdvmpgvn avlkhmrefs dsvrkgqwrg ysgksitdvv nigiggsdlg plmvtealqp
      181 yqcgpnvhfv snidgthlak tlkklsaett lfiiasktft tqetitnaqs akewlmnhtk
      241 npadvakhfv alstngpkvk efgideknmf efwdwvggry slwsaiglsv alfvgmdnfe
      301 qllsgahfmd qhflkapvea nvpiilavlg vwyhnffgae shallpydqy lhrfaayfqq
      361 gdmesngkyi trsgkrvayt tgpivwgepg tngqhafyql ihqgtrlipc dfiapvkthn
      421 pishgihhei llanflaqpe almkgktkee araelekagm sqdvlekilp hkvfegnrpt
      481 nsilvdkvtp fslgmliamy ehkiftqgii wdvnsfdqwg velgkqlaka iqpelkgknq
      541 vsthdsstng linfiksfn
//
LOCUS       XP_077502740             246 aa            linear   INV 25-NOV-2025
DEFINITION  NHP2 ribonucleoprotein [Amblyomma americanum].
ACCESSION   XP_077502740
VERSION     XP_077502740.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646614.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..246
                     /product="NHP2 ribonucleoprotein"
                     /calculated_mol_wt=26770
     Region          137..231
                     /region_name="Ribosomal_L7Ae"
                     /note="Ribosomal protein L7Ae/L30e/S12e/Gadd45 family;
                     pfam01248"
                     /db_xref="CDD:426153"
     CDS             1..246
                     /gene="NHP2"
                     /coded_by="XM_077646614.1:49..789"
                     /db_xref="GeneID:144113511"
ORIGIN      
        1 msflpscggw aavpciqasg vsgcsdcvlf stgnvvlrgn eacalsaycr pallfsmpki
       61 krertedtye egnsvmkvlk kikkekvedg asanvedgld estravengg qdsltyeekl
      121 afvnsiakpm atkkftkkly klvkkaakhk gylmtglkev qgklrkgetg lvilagdvhp
      181 idiishipgl ceekglpyif tpsrrdmata vgmkralvil mikehadyke lmaecaqviq
      241 neyamv
//
LOCUS       XP_077502741             600 aa            linear   INV 25-NOV-2025
DEFINITION  terminal nucleotidyltransferase 4B-like [Amblyomma americanum].
ACCESSION   XP_077502741
VERSION     XP_077502741.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646615.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..600
                     /product="terminal nucleotidyltransferase 4B-like"
                     /calculated_mol_wt=66830
     Region          38..444
                     /region_name="TRF4"
                     /note="DNA polymerase sigma [Replication, recombination
                     and repair]; COG5260"
                     /db_xref="CDD:227585"
     CDS             1..600
                     /gene="LOC144113512"
                     /coded_by="XM_077646615.1:210..2012"
                     /db_xref="GeneID:144113512"
ORIGIN      
        1 mdpsigwyqp ehygpalrlw nsiwetqqgl etmdlnnsnf rapeyipins tvasvtepkk
       61 avngqnelts gadkfynptk rkrenrasty alnrnqhlis qyggtpwrte vkhfrpgilg
      121 lhdeieefyr ymqptpaehr mrlgviqrik dvilglwpqa eveifgsfrt glylptsdid
      181 vvvlgkwetl pmwtlekall shgiaepqsi kvldkasvpi vkltdakttv kvdisfnmnn
      241 gvksacliqs fkekfpalpk lvlvlkqfll qrdlnevftg gissyslilm tvsflqlhpr
      301 cgdapspnlg tllleffdly gkhfnyfvtg irikdggayi rkeemqrdag dsyrpsilci
      361 edpltpgndi grssygaltv kkafeyafmv lnqavhsfhp amsdpkqsil griirvtdev
      421 iqyrqwiqek fpvdnneels tlpppdsect kqeasnrshg krttneadss sstpssgpsf
      481 ggspqhtsat gsdgvsgvrs qgactngvaa vwsqdsegac eaneqladsc qlttsrtffp
      541 kqrkfstgtp eaaaehsscr gfssssarrr qrnlsrptre tvlvaqglvh rdmytvasfr
//
LOCUS       XP_077502742             574 aa            linear   INV 25-NOV-2025
DEFINITION  malonate--CoA ligase ACSF3, mitochondrial-like [Amblyomma
            americanum].
ACCESSION   XP_077502742
VERSION     XP_077502742.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646616.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..574
                     /product="malonate--CoA ligase ACSF3, mitochondrial-like"
                     /calculated_mol_wt=63819
     Region          55..566
                     /region_name="MCS"
                     /note="Malonyl-CoA synthetase (MCS); cd05941"
                     /db_xref="CDD:341264"
     Site            order(192,195..200,202..203)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341264"
     Site            order(195,235..236,239..241,288,290,294,329..331,350..355,
                     359,374,451,462,470,472,474,477,506,543)
                     /site_type="active"
                     /db_xref="CDD:341264"
     Site            order(195,329..331,350..355,374,451,462,470,472,477)
                     /site_type="other"
                     /note="AMP binding site [chemical binding]"
                     /db_xref="CDD:341264"
     Site            order(235..236,239..241,288,290,294,329,351,353..355,359,
                     472,474..476,506,543)
                     /site_type="other"
                     /note="CoA binding site [chemical binding]"
                     /db_xref="CDD:341264"
     CDS             1..574
                     /gene="LOC144113514"
                     /coded_by="XM_077646616.1:500..2224"
                     /db_xref="GeneID:144113514"
ORIGIN      
        1 mtlivfarlq gtllknlslk lklgcassik cassavgnfp pqfspvfggi eehlnklavv
       61 dalgshtygq llgfsqqire qiskatkshd qmrisylcps diryvatqwa cwlggnvavp
      121 lyhqhpdsml eyyikdsqss vllttqeyke rivklaerls lpaividgpd ndsakevenk
      181 dwesfkeeda lmmytsgttg ppkgvvlsfh nvhfqvaqir rawqwtaadv mlhalplhht
      241 hgiisglltp lysraaclml pkfspsevwk hllhldkskp avsvfmavpt myvkliehye
      301 qhlkhsketa pevvqetfak nirfvisgsa slpqpifekf qditgmtile rygmtevgvp
      361 lsnplhgkrh pgcvgyptag vevciaepdn sqlgykplvv gteeetkfle gpdnssgell
      421 irgsnvfkyy wqrpevtkds ftqdgwfktg dsatcidgvy kilgrtsadi iktggykvsa
      481 ldverhllah psikectvvg apddtwgerv aavvvlhdps ttlelealrn wckermpsyn
      541 vpsilhcvpa lernflgkvn kkelvkkmwp apkp
//
LOCUS       XP_077502743             574 aa            linear   INV 25-NOV-2025
DEFINITION  malonate--CoA ligase ACSF3, mitochondrial-like [Amblyomma
            americanum].
ACCESSION   XP_077502743
VERSION     XP_077502743.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646617.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..574
                     /product="malonate--CoA ligase ACSF3, mitochondrial-like"
                     /calculated_mol_wt=63819
     Region          55..566
                     /region_name="MCS"
                     /note="Malonyl-CoA synthetase (MCS); cd05941"
                     /db_xref="CDD:341264"
     Site            order(192,195..200,202..203)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341264"
     Site            order(195,235..236,239..241,288,290,294,329..331,350..355,
                     359,374,451,462,470,472,474,477,506,543)
                     /site_type="active"
                     /db_xref="CDD:341264"
     Site            order(195,329..331,350..355,374,451,462,470,472,477)
                     /site_type="other"
                     /note="AMP binding site [chemical binding]"
                     /db_xref="CDD:341264"
     Site            order(235..236,239..241,288,290,294,329,351,353..355,359,
                     472,474..476,506,543)
                     /site_type="other"
                     /note="CoA binding site [chemical binding]"
                     /db_xref="CDD:341264"
     CDS             1..574
                     /gene="LOC144113514"
                     /coded_by="XM_077646617.1:354..2078"
                     /db_xref="GeneID:144113514"
ORIGIN      
        1 mtlivfarlq gtllknlslk lklgcassik cassavgnfp pqfspvfggi eehlnklavv
       61 dalgshtygq llgfsqqire qiskatkshd qmrisylcps diryvatqwa cwlggnvavp
      121 lyhqhpdsml eyyikdsqss vllttqeyke rivklaerls lpaividgpd ndsakevenk
      181 dwesfkeeda lmmytsgttg ppkgvvlsfh nvhfqvaqir rawqwtaadv mlhalplhht
      241 hgiisglltp lysraaclml pkfspsevwk hllhldkskp avsvfmavpt myvkliehye
      301 qhlkhsketa pevvqetfak nirfvisgsa slpqpifekf qditgmtile rygmtevgvp
      361 lsnplhgkrh pgcvgyptag vevciaepdn sqlgykplvv gteeetkfle gpdnssgell
      421 irgsnvfkyy wqrpevtkds ftqdgwfktg dsatcidgvy kilgrtsadi iktggykvsa
      481 ldverhllah psikectvvg apddtwgerv aavvvlhdps ttlelealrn wckermpsyn
      541 vpsilhcvpa lernflgkvn kkelvkkmwp apkp
//
LOCUS       XP_077502744             574 aa            linear   INV 25-NOV-2025
DEFINITION  malonate--CoA ligase ACSF3, mitochondrial-like [Amblyomma
            americanum].
ACCESSION   XP_077502744
VERSION     XP_077502744.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646618.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..574
                     /product="malonate--CoA ligase ACSF3, mitochondrial-like"
                     /calculated_mol_wt=63819
     Region          55..566
                     /region_name="MCS"
                     /note="Malonyl-CoA synthetase (MCS); cd05941"
                     /db_xref="CDD:341264"
     Site            order(192,195..200,202..203)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341264"
     Site            order(195,235..236,239..241,288,290,294,329..331,350..355,
                     359,374,451,462,470,472,474,477,506,543)
                     /site_type="active"
                     /db_xref="CDD:341264"
     Site            order(195,329..331,350..355,374,451,462,470,472,477)
                     /site_type="other"
                     /note="AMP binding site [chemical binding]"
                     /db_xref="CDD:341264"
     Site            order(235..236,239..241,288,290,294,329,351,353..355,359,
                     472,474..476,506,543)
                     /site_type="other"
                     /note="CoA binding site [chemical binding]"
                     /db_xref="CDD:341264"
     CDS             1..574
                     /gene="LOC144113514"
                     /coded_by="XM_077646618.1:150..1874"
                     /db_xref="GeneID:144113514"
ORIGIN      
        1 mtlivfarlq gtllknlslk lklgcassik cassavgnfp pqfspvfggi eehlnklavv
       61 dalgshtygq llgfsqqire qiskatkshd qmrisylcps diryvatqwa cwlggnvavp
      121 lyhqhpdsml eyyikdsqss vllttqeyke rivklaerls lpaividgpd ndsakevenk
      181 dwesfkeeda lmmytsgttg ppkgvvlsfh nvhfqvaqir rawqwtaadv mlhalplhht
      241 hgiisglltp lysraaclml pkfspsevwk hllhldkskp avsvfmavpt myvkliehye
      301 qhlkhsketa pevvqetfak nirfvisgsa slpqpifekf qditgmtile rygmtevgvp
      361 lsnplhgkrh pgcvgyptag vevciaepdn sqlgykplvv gteeetkfle gpdnssgell
      421 irgsnvfkyy wqrpevtkds ftqdgwfktg dsatcidgvy kilgrtsadi iktggykvsa
      481 ldverhllah psikectvvg apddtwgerv aavvvlhdps ttlelealrn wckermpsyn
      541 vpsilhcvpa lernflgkvn kkelvkkmwp apkp
//
LOCUS       XP_077502746             464 aa            linear   INV 25-NOV-2025
DEFINITION  transport and golgi organization 10 [Amblyomma americanum].
ACCESSION   XP_077502746
VERSION     XP_077502746.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646620.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..464
                     /product="transport and golgi organization 10"
                     /calculated_mol_wt=52956
     Region          20..131
                     /region_name="BTB_POZ_BTBD17"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in BTB/POZ
                     domain-containing protein 17 (BTBD17); cd18292"
                     /db_xref="CDD:349601"
     Region          141..213
                     /region_name="BACK_BTBD17"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     BTB/POZ domain-containing protein 17 (BTBD17); cd18493"
                     /db_xref="CDD:350568"
     CDS             1..464
                     /gene="Tango10"
                     /coded_by="XM_077646620.1:277..1671"
                     /db_xref="GeneID:144113515"
ORIGIN      
        1 mahsndstgd heqqfdnpca vlekiaglya erllsditle vggkqyaahr lilcassdvf
       61 qvmlmdpkws ssrtthiqlq eeplcetvfg dflgylytgr lrvdhlrvlp lvvladkynv
      121 kdlmrlcvny mahhivsaay hnqlvswyry tlncgsssvh vafsnfiawn fdlvastedf
      181 gnidfdvlts flkrsdlvta sefqlfshaa rwlslqepwf qcsqpandea fgqlvrqvma
      241 hvrfpmlnvd elkevlshpl arhykrvmlk rlkialefqt fglmngfrwd wpesmvpriy
      301 tadtwsaslc idnyprqtrf gchtlifmtp sstswaladk vlewtvdvyp kgvlfqrfll
      361 ialretlegp esqtktvrls ltssrsneda tvvvsilvsg mqdgvehvrk vvqrehtftq
      421 esrmlnlddl vpyaelndva assflvgprc drfrlritim pvcs
//
LOCUS       XP_077502747             464 aa            linear   INV 25-NOV-2025
DEFINITION  transport and golgi organization 10 [Amblyomma americanum].
ACCESSION   XP_077502747
VERSION     XP_077502747.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646621.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..464
                     /product="transport and golgi organization 10"
                     /calculated_mol_wt=52956
     Region          20..131
                     /region_name="BTB_POZ_BTBD17"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in BTB/POZ
                     domain-containing protein 17 (BTBD17); cd18292"
                     /db_xref="CDD:349601"
     Region          141..213
                     /region_name="BACK_BTBD17"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     BTB/POZ domain-containing protein 17 (BTBD17); cd18493"
                     /db_xref="CDD:350568"
     CDS             1..464
                     /gene="Tango10"
                     /coded_by="XM_077646621.1:248..1642"
                     /db_xref="GeneID:144113515"
ORIGIN      
        1 mahsndstgd heqqfdnpca vlekiaglya erllsditle vggkqyaahr lilcassdvf
       61 qvmlmdpkws ssrtthiqlq eeplcetvfg dflgylytgr lrvdhlrvlp lvvladkynv
      121 kdlmrlcvny mahhivsaay hnqlvswyry tlncgsssvh vafsnfiawn fdlvastedf
      181 gnidfdvlts flkrsdlvta sefqlfshaa rwlslqepwf qcsqpandea fgqlvrqvma
      241 hvrfpmlnvd elkevlshpl arhykrvmlk rlkialefqt fglmngfrwd wpesmvpriy
      301 tadtwsaslc idnyprqtrf gchtlifmtp sstswaladk vlewtvdvyp kgvlfqrfll
      361 ialretlegp esqtktvrls ltssrsneda tvvvsilvsg mqdgvehvrk vvqrehtftq
      421 esrmlnlddl vpyaelndva assflvgprc drfrlritim pvcs
//
LOCUS       XP_077502748             464 aa            linear   INV 25-NOV-2025
DEFINITION  transport and golgi organization 10 [Amblyomma americanum].
ACCESSION   XP_077502748
VERSION     XP_077502748.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646622.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..464
                     /product="transport and golgi organization 10"
                     /calculated_mol_wt=52956
     Region          20..131
                     /region_name="BTB_POZ_BTBD17"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in BTB/POZ
                     domain-containing protein 17 (BTBD17); cd18292"
                     /db_xref="CDD:349601"
     Region          141..213
                     /region_name="BACK_BTBD17"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     BTB/POZ domain-containing protein 17 (BTBD17); cd18493"
                     /db_xref="CDD:350568"
     CDS             1..464
                     /gene="Tango10"
                     /coded_by="XM_077646622.1:260..1654"
                     /db_xref="GeneID:144113515"
ORIGIN      
        1 mahsndstgd heqqfdnpca vlekiaglya erllsditle vggkqyaahr lilcassdvf
       61 qvmlmdpkws ssrtthiqlq eeplcetvfg dflgylytgr lrvdhlrvlp lvvladkynv
      121 kdlmrlcvny mahhivsaay hnqlvswyry tlncgsssvh vafsnfiawn fdlvastedf
      181 gnidfdvlts flkrsdlvta sefqlfshaa rwlslqepwf qcsqpandea fgqlvrqvma
      241 hvrfpmlnvd elkevlshpl arhykrvmlk rlkialefqt fglmngfrwd wpesmvpriy
      301 tadtwsaslc idnyprqtrf gchtlifmtp sstswaladk vlewtvdvyp kgvlfqrfll
      361 ialretlegp esqtktvrls ltssrsneda tvvvsilvsg mqdgvehvrk vvqrehtftq
      421 esrmlnlddl vpyaelndva assflvgprc drfrlritim pvcs
//
LOCUS       XP_077502749             464 aa            linear   INV 25-NOV-2025
DEFINITION  transport and golgi organization 10 [Amblyomma americanum].
ACCESSION   XP_077502749
VERSION     XP_077502749.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646623.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..464
                     /product="transport and golgi organization 10"
                     /calculated_mol_wt=52956
     Region          20..131
                     /region_name="BTB_POZ_BTBD17"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in BTB/POZ
                     domain-containing protein 17 (BTBD17); cd18292"
                     /db_xref="CDD:349601"
     Region          141..213
                     /region_name="BACK_BTBD17"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     BTB/POZ domain-containing protein 17 (BTBD17); cd18493"
                     /db_xref="CDD:350568"
     CDS             1..464
                     /gene="Tango10"
                     /coded_by="XM_077646623.1:281..1675"
                     /db_xref="GeneID:144113515"
ORIGIN      
        1 mahsndstgd heqqfdnpca vlekiaglya erllsditle vggkqyaahr lilcassdvf
       61 qvmlmdpkws ssrtthiqlq eeplcetvfg dflgylytgr lrvdhlrvlp lvvladkynv
      121 kdlmrlcvny mahhivsaay hnqlvswyry tlncgsssvh vafsnfiawn fdlvastedf
      181 gnidfdvlts flkrsdlvta sefqlfshaa rwlslqepwf qcsqpandea fgqlvrqvma
      241 hvrfpmlnvd elkevlshpl arhykrvmlk rlkialefqt fglmngfrwd wpesmvpriy
      301 tadtwsaslc idnyprqtrf gchtlifmtp sstswaladk vlewtvdvyp kgvlfqrfll
      361 ialretlegp esqtktvrls ltssrsneda tvvvsilvsg mqdgvehvrk vvqrehtftq
      421 esrmlnlddl vpyaelndva assflvgprc drfrlritim pvcs
//
LOCUS       XP_077502750             464 aa            linear   INV 25-NOV-2025
DEFINITION  transport and golgi organization 10 [Amblyomma americanum].
ACCESSION   XP_077502750
VERSION     XP_077502750.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646624.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..464
                     /product="transport and golgi organization 10"
                     /calculated_mol_wt=52956
     Region          20..131
                     /region_name="BTB_POZ_BTBD17"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in BTB/POZ
                     domain-containing protein 17 (BTBD17); cd18292"
                     /db_xref="CDD:349601"
     Region          141..213
                     /region_name="BACK_BTBD17"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     BTB/POZ domain-containing protein 17 (BTBD17); cd18493"
                     /db_xref="CDD:350568"
     CDS             1..464
                     /gene="Tango10"
                     /coded_by="XM_077646624.1:732..2126"
                     /db_xref="GeneID:144113515"
ORIGIN      
        1 mahsndstgd heqqfdnpca vlekiaglya erllsditle vggkqyaahr lilcassdvf
       61 qvmlmdpkws ssrtthiqlq eeplcetvfg dflgylytgr lrvdhlrvlp lvvladkynv
      121 kdlmrlcvny mahhivsaay hnqlvswyry tlncgsssvh vafsnfiawn fdlvastedf
      181 gnidfdvlts flkrsdlvta sefqlfshaa rwlslqepwf qcsqpandea fgqlvrqvma
      241 hvrfpmlnvd elkevlshpl arhykrvmlk rlkialefqt fglmngfrwd wpesmvpriy
      301 tadtwsaslc idnyprqtrf gchtlifmtp sstswaladk vlewtvdvyp kgvlfqrfll
      361 ialretlegp esqtktvrls ltssrsneda tvvvsilvsg mqdgvehvrk vvqrehtftq
      421 esrmlnlddl vpyaelndva assflvgprc drfrlritim pvcs
//
LOCUS       XP_077502751             464 aa            linear   INV 25-NOV-2025
DEFINITION  transport and golgi organization 10 [Amblyomma americanum].
ACCESSION   XP_077502751
VERSION     XP_077502751.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646625.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..464
                     /product="transport and golgi organization 10"
                     /calculated_mol_wt=52956
     Region          20..131
                     /region_name="BTB_POZ_BTBD17"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in BTB/POZ
                     domain-containing protein 17 (BTBD17); cd18292"
                     /db_xref="CDD:349601"
     Region          141..213
                     /region_name="BACK_BTBD17"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     BTB/POZ domain-containing protein 17 (BTBD17); cd18493"
                     /db_xref="CDD:350568"
     CDS             1..464
                     /gene="Tango10"
                     /coded_by="XM_077646625.1:336..1730"
                     /db_xref="GeneID:144113515"
ORIGIN      
        1 mahsndstgd heqqfdnpca vlekiaglya erllsditle vggkqyaahr lilcassdvf
       61 qvmlmdpkws ssrtthiqlq eeplcetvfg dflgylytgr lrvdhlrvlp lvvladkynv
      121 kdlmrlcvny mahhivsaay hnqlvswyry tlncgsssvh vafsnfiawn fdlvastedf
      181 gnidfdvlts flkrsdlvta sefqlfshaa rwlslqepwf qcsqpandea fgqlvrqvma
      241 hvrfpmlnvd elkevlshpl arhykrvmlk rlkialefqt fglmngfrwd wpesmvpriy
      301 tadtwsaslc idnyprqtrf gchtlifmtp sstswaladk vlewtvdvyp kgvlfqrfll
      361 ialretlegp esqtktvrls ltssrsneda tvvvsilvsg mqdgvehvrk vvqrehtftq
      421 esrmlnlddl vpyaelndva assflvgprc drfrlritim pvcs
//
LOCUS       XP_077502752             464 aa            linear   INV 25-NOV-2025
DEFINITION  transport and golgi organization 10 [Amblyomma americanum].
ACCESSION   XP_077502752
VERSION     XP_077502752.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646626.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..464
                     /product="transport and golgi organization 10"
                     /calculated_mol_wt=52956
     Region          20..131
                     /region_name="BTB_POZ_BTBD17"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in BTB/POZ
                     domain-containing protein 17 (BTBD17); cd18292"
                     /db_xref="CDD:349601"
     Region          141..213
                     /region_name="BACK_BTBD17"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     BTB/POZ domain-containing protein 17 (BTBD17); cd18493"
                     /db_xref="CDD:350568"
     CDS             1..464
                     /gene="Tango10"
                     /coded_by="XM_077646626.1:340..1734"
                     /db_xref="GeneID:144113515"
ORIGIN      
        1 mahsndstgd heqqfdnpca vlekiaglya erllsditle vggkqyaahr lilcassdvf
       61 qvmlmdpkws ssrtthiqlq eeplcetvfg dflgylytgr lrvdhlrvlp lvvladkynv
      121 kdlmrlcvny mahhivsaay hnqlvswyry tlncgsssvh vafsnfiawn fdlvastedf
      181 gnidfdvlts flkrsdlvta sefqlfshaa rwlslqepwf qcsqpandea fgqlvrqvma
      241 hvrfpmlnvd elkevlshpl arhykrvmlk rlkialefqt fglmngfrwd wpesmvpriy
      301 tadtwsaslc idnyprqtrf gchtlifmtp sstswaladk vlewtvdvyp kgvlfqrfll
      361 ialretlegp esqtktvrls ltssrsneda tvvvsilvsg mqdgvehvrk vvqrehtftq
      421 esrmlnlddl vpyaelndva assflvgprc drfrlritim pvcs
//
LOCUS       XP_077502753             818 aa            linear   INV 25-NOV-2025
DEFINITION  vacuolar protein sorting 35 [Amblyomma americanum].
ACCESSION   XP_077502753
VERSION     XP_077502753.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646627.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..818
                     /product="vacuolar protein sorting 35"
                     /calculated_mol_wt=93344
     Region          16..774
                     /region_name="Vps35"
                     /note="Vacuolar protein sorting-associated protein 35;
                     pfam03635"
                     /db_xref="CDD:427414"
     CDS             1..818
                     /gene="Vps35"
                     /coded_by="XM_077646627.1:202..2658"
                     /db_xref="GeneID:144113516"
ORIGIN      
        1 mpqtpqaspq edqeklldda asivkvqafh mkrcldkgkl mealkhasnm lgelrtslls
       61 pksyyelyma vtdelrhlei hlldeiqkgr kmsdlyelvq yagniiprly llitvglvym
      121 ksnehskkdi lkdlvemcrg vqhplrglfl rnyllqctrn ilpdneedls lndanlnadg
      181 gkvellmysr esevsgtvkd svdfvllnfg emnklwvrmq hqghsrdrdr rekerqelrl
      241 lvgtnlvrls qldavdmdry kkvvlpgile qvvscrdaia qeylmeciiq vfpdefhlqt
      301 lscflkacae lrpqvnvkni iislidrlaa yamkedgpgi psdiklfdif sdqiaqviqm
      361 rqdmptediv slqvslihla lkcyrervdy vdkvlqttee iftklgitrv elskpvgkel
      421 vrllkipvqs ygdlltilql shfgsllqlc dyrgrkvmas flvnsalend tliptpeqad
      481 rvlilisplv qdqpdqpdee edpedfleeq vlvgrfanlm vsdspdqqyl ivmtarkhfg
      541 sggnkriryt lpplvfqsyq lafkylslsd qddkwekkcn kifqfchqti salikaelae
      601 lplrlflqga lvagqikfen yetvayefis qafslyedei sdskaqlsai tlimgtveqt
      661 scfceenhep lrtqcalaas kllkkpdqcr gvglcshlfw sgktqesgge emhdgkrvie
      721 clkkglriat qcmdssvqvq lfvellnyyi yffekgneqi ktdtisqlig kireelpqle
      781 aneetdqikk hfqntldhlr armespdtdg psyaglsl
//
LOCUS       XP_077502754             818 aa            linear   INV 25-NOV-2025
DEFINITION  vacuolar protein sorting 35 [Amblyomma americanum].
ACCESSION   XP_077502754
VERSION     XP_077502754.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646628.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..818
                     /product="vacuolar protein sorting 35"
                     /calculated_mol_wt=93344
     Region          16..774
                     /region_name="Vps35"
                     /note="Vacuolar protein sorting-associated protein 35;
                     pfam03635"
                     /db_xref="CDD:427414"
     CDS             1..818
                     /gene="Vps35"
                     /coded_by="XM_077646628.1:202..2658"
                     /db_xref="GeneID:144113516"
ORIGIN      
        1 mpqtpqaspq edqeklldda asivkvqafh mkrcldkgkl mealkhasnm lgelrtslls
       61 pksyyelyma vtdelrhlei hlldeiqkgr kmsdlyelvq yagniiprly llitvglvym
      121 ksnehskkdi lkdlvemcrg vqhplrglfl rnyllqctrn ilpdneedls lndanlnadg
      181 gkvellmysr esevsgtvkd svdfvllnfg emnklwvrmq hqghsrdrdr rekerqelrl
      241 lvgtnlvrls qldavdmdry kkvvlpgile qvvscrdaia qeylmeciiq vfpdefhlqt
      301 lscflkacae lrpqvnvkni iislidrlaa yamkedgpgi psdiklfdif sdqiaqviqm
      361 rqdmptediv slqvslihla lkcyrervdy vdkvlqttee iftklgitrv elskpvgkel
      421 vrllkipvqs ygdlltilql shfgsllqlc dyrgrkvmas flvnsalend tliptpeqad
      481 rvlilisplv qdqpdqpdee edpedfleeq vlvgrfanlm vsdspdqqyl ivmtarkhfg
      541 sggnkriryt lpplvfqsyq lafkylslsd qddkwekkcn kifqfchqti salikaelae
      601 lplrlflqga lvagqikfen yetvayefis qafslyedei sdskaqlsai tlimgtveqt
      661 scfceenhep lrtqcalaas kllkkpdqcr gvglcshlfw sgktqesgge emhdgkrvie
      721 clkkglriat qcmdssvqvq lfvellnyyi yffekgneqi ktdtisqlig kireelpqle
      781 aneetdqikk hfqntldhlr armespdtdg psyaglsl
//
LOCUS       XP_077502755             475 aa            linear   INV 25-NOV-2025
DEFINITION  pyruvate dehydrogenase [acetyl-transferring]-phosphatase 1-like
            protein, mitochondrial [Amblyomma americanum].
ACCESSION   XP_077502755
VERSION     XP_077502755.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646629.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..475
                     /product="pyruvate dehydrogenase
                     [acetyl-transferring]-phosphatase 1-like protein,
                     mitochondrial"
                     /calculated_mol_wt=52861
     Region          70..362
                     /region_name="PP2Cc"
                     /note="Serine/threonine phosphatases, family 2C, catalytic
                     domain; The protein architecture and deduced catalytic
                     mechanism of PP2C phosphatases are similar to the PP1,
                     PP2A, PP2B family of protein Ser/Thr phosphatases, with
                     which PP2C shares no sequence...; cd00143"
                     /db_xref="CDD:238083"
     Site            order(80,84..85,104..106,358)
                     /site_type="active"
                     /db_xref="CDD:238083"
     CDS             1..475
                     /gene="Pdp"
                     /coded_by="XM_077646629.1:193..1620"
                     /db_xref="GeneID:144113518"
ORIGIN      
        1 mattltglrh fslprlqria palcvclnvp kqlsrsrsdl fgaprlgppe vskilranet
       61 srevqarslr sfdtnqlpsn spmedrlvia rcllttghlf gvfdghgghg faqlvsqrlf
      121 dyialsilph allkeyieqn rrthlvevih cidsmtdeqn qahfdslhaf ahellssmgr
      181 pfsmqdalhq aflqldadis revaeqkwqd slryalmgac acvvhvdgmh lhvasagdcr
      241 avlgclteds swqakplcle hntdnigelr rvlaehpese rntvvrqdrl lgqlaplral
      301 gdfnykwpas qvaellvpla gphalpphyc tppyltavpe vthhhlgphd kflvlasdgl
      361 weqlqphrva klvgqhmsgr qtldrlrlpq phmrliqvar ilaarqkgla qkptdanaat
      421 hlirnalgrt eygiehskla amlalpqevv rsfrddisia viyfdsnflr lspag
//
LOCUS       XP_077502756             340 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113519 [Amblyomma americanum].
ACCESSION   XP_077502756
VERSION     XP_077502756.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646630.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..340
                     /product="uncharacterized protein LOC144113519"
                     /calculated_mol_wt=37562
     Region          <284..320
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          298..302
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          312..317
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          326..329
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..340
                     /gene="LOC144113519"
                     /coded_by="XM_077646630.1:1856..2878"
                     /db_xref="GeneID:144113519"
ORIGIN      
        1 mapqrgasag adksddrhpf rrplanarll vtpglvirrp wrqarkavla rfvgrsawlm
       61 prdgpsflae kkksrgsrda arqcpdtram rtalllcllt averrrssas hhhhhappqt
      121 dcyhqywlyq paqavggfqy pyaqsgrhgd ypypyapdhh rgypdgrypv aelppppppp
      181 ppppppslpy pgpwvppypr qpapknldpf pgkflgmgsv rylnggrdve lvcdlhgnql
      241 vsnvvwvkat hprgqppawr ppppscgpcf cpvqygcvyq pldladrrff adsrgnhasl
      301 hiygvspady gvyrcsatss ptgsaadnte tvyqivhftg
//
LOCUS       XP_077502757             382 aa            linear   INV 25-NOV-2025
DEFINITION  glucose-fructose oxidoreductase domain-containing protein 2
            [Amblyomma americanum].
ACCESSION   XP_077502757
VERSION     XP_077502757.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646631.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..382
                     /product="glucose-fructose oxidoreductase
                     domain-containing protein 2"
                     /calculated_mol_wt=41766
     Region          4..358
                     /region_name="MviM"
                     /note="Predicted dehydrogenase [General function
                     prediction only]; COG0673"
                     /db_xref="CDD:440437"
     CDS             1..382
                     /gene="LOC144113520"
                     /coded_by="XM_077646631.1:209..1357"
                     /db_xref="GeneID:144113520"
ORIGIN      
        1 mlpgvgvfgt gksvrvlvpc lrsrgfkvea iwgrtadtak eaalelgipf ytnkedevll
       61 rksvdfvcim csphlhaqia vkalgigkhv lcerpaglsq sevlkmvraa qyyptlisvl
      121 chslrflpay vhmkrqiqdg yvgtvqiiea rvhcgslvhd rydwtceesm gggvlstfgs
      181 hvidiaafvt glravrvhgl trtltkttdt vcgirqvssd dfctfqmels ngayatvtln
      241 shcagqfsqe llvcgtrgnl vvrsgdlhgq rkgakeevly ldvedlkqtp dsvvpkphak
      301 glvkmvtalr dafasrghgq gwakepadsa asfedglyiq avmeavrqss qarqwvrvql
      361 lqdearhkga fcrlyrmasa sm
//
LOCUS       XP_077502758              99 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113521 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502758
VERSION     XP_077502758.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646632.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..99
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..99
                     /product="uncharacterized protein LOC144113521 isoform X1"
                     /calculated_mol_wt=10689
     CDS             1..99
                     /gene="LOC144113521"
                     /coded_by="XM_077646632.1:141..440"
                     /db_xref="GeneID:144113521"
ORIGIN      
        1 mkhqnsedsp vneedgesvk glkrmrlgdm dqpcsallqs fkesilakiq mlegrldals
       61 dqcetmedki ellstavkeh asapsprlgs vggntfsll
//
LOCUS       XP_077502759             108 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113521 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502759
VERSION     XP_077502759.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646633.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..108
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..108
                     /product="uncharacterized protein LOC144113521 isoform X2"
                     /calculated_mol_wt=11749
     CDS             1..108
                     /gene="LOC144113521"
                     /coded_by="XM_077646633.1:130..456"
                     /db_xref="GeneID:144113521"
ORIGIN      
        1 mrsstperdm khqnsedspv needgesvkg lkrmrlgdmd qpcsallqsf kesilakiqm
       61 legrldalsd qcetmedkie llstavkeha sapsprlgsv ggntfsll
//
LOCUS       XP_077502760              99 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113521 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502760
VERSION     XP_077502760.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646634.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..99
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..99
                     /product="uncharacterized protein LOC144113521 isoform X1"
                     /calculated_mol_wt=10689
     CDS             1..99
                     /gene="LOC144113521"
                     /coded_by="XM_077646634.1:109..408"
                     /db_xref="GeneID:144113521"
ORIGIN      
        1 mkhqnsedsp vneedgesvk glkrmrlgdm dqpcsallqs fkesilakiq mlegrldals
       61 dqcetmedki ellstavkeh asapsprlgs vggntfsll
//
LOCUS       XP_077502761              99 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113521 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502761
VERSION     XP_077502761.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646635.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..99
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..99
                     /product="uncharacterized protein LOC144113521 isoform X1"
                     /calculated_mol_wt=10689
     CDS             1..99
                     /gene="LOC144113521"
                     /coded_by="XM_077646635.1:173..472"
                     /db_xref="GeneID:144113521"
ORIGIN      
        1 mkhqnsedsp vneedgesvk glkrmrlgdm dqpcsallqs fkesilakiq mlegrldals
       61 dqcetmedki ellstavkeh asapsprlgs vggntfsll
//
LOCUS       XP_077502762             136 aa            linear   INV 25-NOV-2025
DEFINITION  protein BANP-like [Amblyomma americanum].
ACCESSION   XP_077502762
VERSION     XP_077502762.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646636.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..136
                     /product="protein BANP-like"
                     /calculated_mol_wt=15394
     Region          1..72
                     /region_name="BEN"
                     /note="The BEN domain is found in diverse animal proteins;
                     smart01025"
                     /db_xref="CDD:214981"
     CDS             1..136
                     /gene="LOC144113522"
                     /coded_by="XM_077646636.1:260..670"
                     /db_xref="GeneID:144113522"
ORIGIN      
        1 maltlldhlf dretqarsni sgsgkhkkqq ldplmiygir chlrhvfevt nsdwdrikln
       61 idskcrtafr rkkkglplnp kvfctengdf hvvhataeqi aqiqqthhiq ilngnhilat
      121 tvdrapddav rvtvlp
//
LOCUS       XP_077502763             522 aa            linear   INV 25-NOV-2025
DEFINITION  protein unc-93 homolog A-like [Amblyomma americanum].
ACCESSION   XP_077502763
VERSION     XP_077502763.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646637.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..522
                     /product="protein unc-93 homolog A-like"
                     /calculated_mol_wt=55412
     Region          43..461
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(51..52,55..56,59,88,138..139,141..143,146,180,
                     183..184,187,298,301..302,305..307,310,337,341,385..386,
                     390,394,417,420..421,424..425,428)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340964"
     CDS             1..522
                     /gene="LOC144113523"
                     /coded_by="XM_077646637.1:292..1860"
                     /db_xref="GeneID:144113523"
ORIGIN      
        1 mqasspgaal peprgsvgvl sppsapswdp rqrrqrlrrn llvvsacsfl vnsaflslsh
       61 lqtsvlnves glgtvslsai sssfvlsclf lapffisvag lkvtigactl lytpyfianf
      121 yphwvtvlpt sfvlgfgagp lctansayva elatqyaglh rqntdvalgr ffgvflmfgq
      181 lsqvfgnlls yvvlgagsat dgsvvsqeas sdkratcgvn fcpqsalptn itapsvtsrf
      241 qlcvvytafg wiaalvafll ldpverkvqr qdrtslhllm ltmrhmrnpy qifiipltif
      301 sglevafiad dfakayitct wgvhyvglfl vslgvsgalf slsfgwllnl tgrsqmvllg
      361 avvnvamiil mlsyetgyll lvllvasgvw giadaawttn iisfyavafw qnpeaafasc
      421 hlwrsvgaia slgysnlvct dmklyillca ltvgvagyfa vdaevkrssa avalpnatvv
      481 natvtpivsa aalqplpaas sldagipvvi palltsnplv aq
//
LOCUS       XP_077502764             467 aa            linear   INV 25-NOV-2025
DEFINITION  UNC93-like protein [Amblyomma americanum].
ACCESSION   XP_077502764
VERSION     XP_077502764.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646638.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..467
                     /product="UNC93-like protein"
                     /calculated_mol_wt=51468
     Region          34..451
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(44..45,48..49,52,80,130..131,133..135,138,172,
                     175..176,179,285,288..289,292..294,297,324,328,373..374,
                     378,382,405,408..409,412..413,416)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340964"
     CDS             1..467
                     /gene="LOC144113524"
                     /coded_by="XM_077646638.1:221..1624"
                     /db_xref="GeneID:144113524"
ORIGIN      
        1 meknpekvla agpevmched ecvegtprki iknvlvisfg fvllfsafqs vtnlqssins
       61 eaglgtftma tiyvalvlsc mfvptllirk lslkntlvvs mamytfyfaa nfyptwgtli
      121 pasilmglgg aplwtskcay lttvaneyav ktrqkpedvv trffglffmi fqtaqiwgnl
      181 isyyvlkpas rpsnasvnia scgisfvtae ddadnenlqr pddtklytlm siltacavla
      241 vfsmllmldp laaparlvsk kpvsllvetl rhlrkpyqwa ilpltvysgv qqafliadfn
      301 qayvscalgi hyvgfimity gvadaifsmg fglitkvigr vpvfifgsvv ngailvvmct
      361 wrptpddfyv ffviagcwgi gdavwqtqvn afygtvftdd eeaafanyrl wesfgfiigf
      421 aiqkvlaihh kvvvvvvflv vgmlgylsie ihlkfkkksq ltvtiss
//
LOCUS       XP_077502765             467 aa            linear   INV 25-NOV-2025
DEFINITION  UNC93-like protein [Amblyomma americanum].
ACCESSION   XP_077502765
VERSION     XP_077502765.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646639.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..467
                     /product="UNC93-like protein"
                     /calculated_mol_wt=51468
     Region          34..451
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(44..45,48..49,52,80,130..131,133..135,138,172,
                     175..176,179,285,288..289,292..294,297,324,328,373..374,
                     378,382,405,408..409,412..413,416)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340964"
     CDS             1..467
                     /gene="LOC144113524"
                     /coded_by="XM_077646639.1:247..1650"
                     /db_xref="GeneID:144113524"
ORIGIN      
        1 meknpekvla agpevmched ecvegtprki iknvlvisfg fvllfsafqs vtnlqssins
       61 eaglgtftma tiyvalvlsc mfvptllirk lslkntlvvs mamytfyfaa nfyptwgtli
      121 pasilmglgg aplwtskcay lttvaneyav ktrqkpedvv trffglffmi fqtaqiwgnl
      181 isyyvlkpas rpsnasvnia scgisfvtae ddadnenlqr pddtklytlm siltacavla
      241 vfsmllmldp laaparlvsk kpvsllvetl rhlrkpyqwa ilpltvysgv qqafliadfn
      301 qayvscalgi hyvgfimity gvadaifsmg fglitkvigr vpvfifgsvv ngailvvmct
      361 wrptpddfyv ffviagcwgi gdavwqtqvn afygtvftdd eeaafanyrl wesfgfiigf
      421 aiqkvlaihh kvvvvvvflv vgmlgylsie ihlkfkkksq ltvtiss
//
LOCUS       XP_077502766             309 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113525 [Amblyomma americanum].
ACCESSION   XP_077502766
VERSION     XP_077502766.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646640.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..309
                     /product="uncharacterized protein LOC144113525"
                     /calculated_mol_wt=33341
     Region          <125..>274
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          185..205
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(185,188,201,205)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          211..231
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(211,214,227,231)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(216,218,220,222..223,226..227,230,244,246,250..251,
                     254..255,258,272,274,276,278..279,282..283)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          239..259
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          267..284
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..309
                     /gene="LOC144113525"
                     /coded_by="XM_077646640.1:139..1068"
                     /db_xref="GeneID:144113525"
ORIGIN      
        1 mprsflvkkv kypvanhhag gprwyrqpss pteaapvpsp qpsspsrgga valpaydysc
       61 pgyeggspgl apvtvlpgge pspvaaeakq yhelspafqf danenvasnv dvvssepstp
      121 lggdkpplad grsrrkvrds sprakrsper prytcgecgk hyatssnlch kqthrspdsq
      181 lakkcptcak vyvsmpalam hvlthnlshk cpvcgkafsr pwllqghmrs htgekpfgca
      241 hcgkafadrs nlrahmqths glkhfrcarc hksfalksyl nkhlesacfk edgcvswaad
      301 dsldalppl
//
LOCUS       XP_077502767             367 aa            linear   INV 25-NOV-2025
DEFINITION  transcriptional repressor scratch 1-like [Amblyomma americanum].
ACCESSION   XP_077502767
VERSION     XP_077502767.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646641.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..367
                     /product="transcriptional repressor scratch 1-like"
                     /calculated_mol_wt=39078
     Region          196..218
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          198..218
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(198,201,214,218)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          229..246
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Region          249..>332
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          255..275
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(255,258,271,275)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(260,262,264,266..267,270..271,274,288,290,294..295,
                     298..299,302,316,318,320,322..323,326..327)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          283..303
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          311..328
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..367
                     /gene="LOC144113526"
                     /coded_by="XM_077646641.1:171..1274"
                     /db_xref="GeneID:144113526"
ORIGIN      
        1 mprcylvkka atkhpagpas sgrwpyrepl spseaetapc aplfgdqaad erpavvtsaa
       61 fgrtsptagf ihdystgqla lgaeslvape raaaysplrd eprvldlsgp efysraplap
      121 lvqaaaeprc pssegarsss passcssfga rstgavtvsy tydaffisdg rsrrrtavsa
      181 lsgparrpat edrprytcge cgkhyatssn lsrhkqthrs pdsqlakkcp tcakvyvsmp
      241 alamhvlthn lshkcpvcgk afsrpwllqg hmrshtgekp fgcahcgkaf adrsnlrahm
      301 qthsglkhfr carchksfal ksylnkhles acfkdgatps ppspalplpp aehqlgpama
      361 avlglat
//
LOCUS       XP_077502768             340 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113527 [Amblyomma americanum].
ACCESSION   XP_077502768
VERSION     XP_077502768.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646642.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..340
                     /product="uncharacterized protein LOC144113527"
                     /calculated_mol_wt=37225
     Region          245..310
                     /region_name="bZIP_Maf_large"
                     /note="Basic leucine zipper (bZIP) domain of large
                     musculoaponeurotic fibrosarcoma (Maf) proteins: a
                     DNA-binding and dimerization domain; cd14718"
                     /db_xref="CDD:269866"
     Region          245..310
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269866"
     Site            order(252,255..257,259..261,263..265,267..268,270..271)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269866"
     Site            order(271,278,281..282,285..286,288..289,292..293,
                     295..296,299,302..303,306..307,309..310)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269866"
     CDS             1..340
                     /gene="LOC144113527"
                     /coded_by="XM_077646642.1:355..1377"
                     /db_xref="GeneID:144113527"
ORIGIN      
        1 mwgkpagael fgaqvvcgar rerrslvsps rargasvaly ageageaava awlggappdp
       61 aclyfqggaa aatpspsgss lsssldedam aadeysfdle nleevvkqem gakyaalqpe
      121 lqqlehlrrp lvgstpgtpp dtppgstacp aspefgacmp ddsmmwlsqp ppppaphqah
      181 vrfghqepld lrpqgdlewl qlrrdlegpr ppmpppmpmr dilddeqlis lsvrelnkrl
      241 hgfpreevvr lkqkrrtlkn rgyaqncrtk rlaqrheles rnrilqaean rlrqelerac
      301 qerdfykqql gaaararaqq pqpqqlpphg lgpgspgflm
//
LOCUS       XP_077502769             123 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113528 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502769
VERSION     XP_077502769.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646643.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..123
                     /product="uncharacterized protein LOC144113528 isoform X1"
                     /calculated_mol_wt=13454
     CDS             1..123
                     /gene="LOC144113528"
                     /coded_by="XM_077646643.1:385..756"
                     /db_xref="GeneID:144113528"
ORIGIN      
        1 mqprigkkpe gfmadgmeer wlsrsgnfne akkktqvrna ddsqcsqkht wrcrvlpsel
       61 pstagrlrlh gghpallthq rrgktsvlpl gitgasalcl lqalspsspa wcprarratn
      121 eis
//
LOCUS       XP_077502770              87 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113528 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502770
VERSION     XP_077502770.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646644.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..87
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..87
                     /product="uncharacterized protein LOC144113528 isoform X2"
                     /calculated_mol_wt=9449
     CDS             1..87
                     /gene="LOC144113528"
                     /coded_by="XM_077646644.1:385..648"
                     /db_xref="GeneID:144113528"
ORIGIN      
        1 mqprigkkpe gfmadgmeer wlsrsgnfne akkktqmtan aarnipggag ccqaschrrp
       61 ggfdytgatp rssrisegek rasfpwe
//
LOCUS       XP_077502771            1416 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113530 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502771
VERSION     XP_077502771.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646645.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1416
                     /product="uncharacterized protein LOC144113530 isoform X1"
                     /calculated_mol_wt=154850
     Region          18..82
                     /region_name="CAP_GLY"
                     /note="CAP-Gly domain; pfam01302"
                     /db_xref="CDD:460154"
     Region          927..1007
                     /region_name="Ubl_HOIL1"
                     /note="ubiquitin-like (Ubl) domain found in heme-oxidized
                     IRP2 ubiquitin ligase 1 (HOIL-1) and similar proteins;
                     cd01799"
                     /db_xref="CDD:340497"
     Site            order(929,934..936,938..940,942..945,964..965,967..968,
                     1005..1007)
                     /site_type="other"
                     /note="UBA interaction site [polypeptide binding]"
                     /db_xref="CDD:340497"
     Region          1179..1234
                     /region_name="mRING-HC-C3HC3D_RBR_HOIL1"
                     /note="Modified RING finger, HC subclass (C3HC3D-type),
                     found in heme-oxidized IRP2 ubiquitin ligase 1 (HOIL-1)
                     and similar proteins; cd16633"
                     /db_xref="CDD:438295"
     Region          1267..1330
                     /region_name="BRcat_RBR_HOIL1"
                     /note="BRcat domain found in heme-oxidized IRP2 ubiquitin
                     ligase 1 (HOIL-1) and similar proteins; cd20345"
                     /db_xref="CDD:439006"
     Region          1344..1416
                     /region_name="Rcat_RBR_HOIL1"
                     /note="Rcat domain found in heme-oxidized IRP2 ubiquitin
                     ligase 1 (HOIL-1) and similar proteins; cd20358"
                     /db_xref="CDD:439019"
     CDS             1..1416
                     /gene="LOC144113530"
                     /coded_by="XM_077646645.1:536..4786"
                     /db_xref="GeneID:144113530"
ORIGIN      
        1 mslrrpfsed dtgplltlgd rvvwlfehgp ehgwvrwigr ipvvcpswti gvefdnpiga
       61 gdgkfggkry fyarndhayf lpnssllras dynscfrkqn tmggptpspq skmytfphqf
      121 saakcpfpgs anaveathlt aahlissgsn skelgicsag kllrsqstca qdghnlsdyd
      181 nwlggqdpcn vhvkswksss thsvvscppv llpkmlvare ptsssdsemn fgslrslmsc
      241 ftlgshrkkk rrkkkkknhr lvivtgpans nccysggnia saqaktaahl cdgehsikhg
      301 akeldakels cvpsrskamw ryslqeqdmq rreasskepv ashsaeqrmp pklppktstk
      361 kkrlapqppd gtvtpvgtlp rnspvavpsg ssahgtdsls ltrrrshkkp apqppgcdmw
      421 hstarsgtlp sgiqkgvyan enteenkgma tlkakplpvd dvgmcgssll qpyslkcqln
      481 vqenvlnpqe kqdfgknlvs lvikpdlpvk tipspklnmg ndkvattgas ssiqnkdlnd
      541 hqgakafgev gkptlaktvv tenekrdayk hqafdrelrs stlpstrqcs pileeeepya
      601 ttavcgefnn lesvlkrnsn vfelldafsk lelnsdetpk dsepespkhq kkqvkslksl
      661 fqkhpadask vvstkwtytp meakkenlfa atkptfpiat rpgdygntyt kventdpssl
      721 csethstkks psqlplntpl ascrmnlkpv shspdfgnvf cklrsqasgs sgnppwknql
      781 qalkampdep rpstselpvv etknkpsggr kegwgslvge sascsvvaat agfplevmag
      841 kaspasspvp sslssahinp grqrppahpn sptalmqakl palfrqleea iskgehdraa
      901 ilarelamyk vscslrrvrk vapdpkqftl kmyvedrksh vgpislpvyp emtlsnlkkk
      961 veaeynipvr vqrwilgknl atddeatleq fgvsqgacpv flylvspaee lkaeevlrns
     1021 swkppdsvas stdevdelap talrqapsda sstldallnd qaagwtcqlc ginntepdtc
     1081 amcgvtalad aidhstvdas hrtavdtdpd aepilrhdle pieihanenn qlgvdndpve
     1141 vdfpqgqqdg tnaevpkqpp qpllpqdymr lveldgqdlv psmeafecsv cflgvepgqg
     1201 vllrdclhmf crdclsnsvr yaeeamvkcp frndeyscss hlqereiral vspevyehhl
     1261 srsvktaesq apnsfhckta dcpgwcmled nvnvflcpvc ghsncltcra ihegknclqy
     1321 qdeldfnapn gqeakqtkey ldnmvkegqa mhcpqcrviv mkkwgcdwlk csvcqteicw
     1381 vtkgprwgpq gkgdtsagck cgvngvkchp kcnych
//
LOCUS       XP_077502772            1416 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113530 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502772
VERSION     XP_077502772.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646646.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1416
                     /product="uncharacterized protein LOC144113530 isoform X1"
                     /calculated_mol_wt=154850
     Region          18..82
                     /region_name="CAP_GLY"
                     /note="CAP-Gly domain; pfam01302"
                     /db_xref="CDD:460154"
     Region          927..1007
                     /region_name="Ubl_HOIL1"
                     /note="ubiquitin-like (Ubl) domain found in heme-oxidized
                     IRP2 ubiquitin ligase 1 (HOIL-1) and similar proteins;
                     cd01799"
                     /db_xref="CDD:340497"
     Site            order(929,934..936,938..940,942..945,964..965,967..968,
                     1005..1007)
                     /site_type="other"
                     /note="UBA interaction site [polypeptide binding]"
                     /db_xref="CDD:340497"
     Region          1179..1234
                     /region_name="mRING-HC-C3HC3D_RBR_HOIL1"
                     /note="Modified RING finger, HC subclass (C3HC3D-type),
                     found in heme-oxidized IRP2 ubiquitin ligase 1 (HOIL-1)
                     and similar proteins; cd16633"
                     /db_xref="CDD:438295"
     Region          1267..1330
                     /region_name="BRcat_RBR_HOIL1"
                     /note="BRcat domain found in heme-oxidized IRP2 ubiquitin
                     ligase 1 (HOIL-1) and similar proteins; cd20345"
                     /db_xref="CDD:439006"
     Region          1344..1416
                     /region_name="Rcat_RBR_HOIL1"
                     /note="Rcat domain found in heme-oxidized IRP2 ubiquitin
                     ligase 1 (HOIL-1) and similar proteins; cd20358"
                     /db_xref="CDD:439019"
     CDS             1..1416
                     /gene="LOC144113530"
                     /coded_by="XM_077646646.1:336..4586"
                     /db_xref="GeneID:144113530"
ORIGIN      
        1 mslrrpfsed dtgplltlgd rvvwlfehgp ehgwvrwigr ipvvcpswti gvefdnpiga
       61 gdgkfggkry fyarndhayf lpnssllras dynscfrkqn tmggptpspq skmytfphqf
      121 saakcpfpgs anaveathlt aahlissgsn skelgicsag kllrsqstca qdghnlsdyd
      181 nwlggqdpcn vhvkswksss thsvvscppv llpkmlvare ptsssdsemn fgslrslmsc
      241 ftlgshrkkk rrkkkkknhr lvivtgpans nccysggnia saqaktaahl cdgehsikhg
      301 akeldakels cvpsrskamw ryslqeqdmq rreasskepv ashsaeqrmp pklppktstk
      361 kkrlapqppd gtvtpvgtlp rnspvavpsg ssahgtdsls ltrrrshkkp apqppgcdmw
      421 hstarsgtlp sgiqkgvyan enteenkgma tlkakplpvd dvgmcgssll qpyslkcqln
      481 vqenvlnpqe kqdfgknlvs lvikpdlpvk tipspklnmg ndkvattgas ssiqnkdlnd
      541 hqgakafgev gkptlaktvv tenekrdayk hqafdrelrs stlpstrqcs pileeeepya
      601 ttavcgefnn lesvlkrnsn vfelldafsk lelnsdetpk dsepespkhq kkqvkslksl
      661 fqkhpadask vvstkwtytp meakkenlfa atkptfpiat rpgdygntyt kventdpssl
      721 csethstkks psqlplntpl ascrmnlkpv shspdfgnvf cklrsqasgs sgnppwknql
      781 qalkampdep rpstselpvv etknkpsggr kegwgslvge sascsvvaat agfplevmag
      841 kaspasspvp sslssahinp grqrppahpn sptalmqakl palfrqleea iskgehdraa
      901 ilarelamyk vscslrrvrk vapdpkqftl kmyvedrksh vgpislpvyp emtlsnlkkk
      961 veaeynipvr vqrwilgknl atddeatleq fgvsqgacpv flylvspaee lkaeevlrns
     1021 swkppdsvas stdevdelap talrqapsda sstldallnd qaagwtcqlc ginntepdtc
     1081 amcgvtalad aidhstvdas hrtavdtdpd aepilrhdle pieihanenn qlgvdndpve
     1141 vdfpqgqqdg tnaevpkqpp qpllpqdymr lveldgqdlv psmeafecsv cflgvepgqg
     1201 vllrdclhmf crdclsnsvr yaeeamvkcp frndeyscss hlqereiral vspevyehhl
     1261 srsvktaesq apnsfhckta dcpgwcmled nvnvflcpvc ghsncltcra ihegknclqy
     1321 qdeldfnapn gqeakqtkey ldnmvkegqa mhcpqcrviv mkkwgcdwlk csvcqteicw
     1381 vtkgprwgpq gkgdtsagck cgvngvkchp kcnych
//
LOCUS       XP_077502773            1416 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113530 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502773
VERSION     XP_077502773.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646647.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1416
                     /product="uncharacterized protein LOC144113530 isoform X1"
                     /calculated_mol_wt=154850
     Region          18..82
                     /region_name="CAP_GLY"
                     /note="CAP-Gly domain; pfam01302"
                     /db_xref="CDD:460154"
     Region          927..1007
                     /region_name="Ubl_HOIL1"
                     /note="ubiquitin-like (Ubl) domain found in heme-oxidized
                     IRP2 ubiquitin ligase 1 (HOIL-1) and similar proteins;
                     cd01799"
                     /db_xref="CDD:340497"
     Site            order(929,934..936,938..940,942..945,964..965,967..968,
                     1005..1007)
                     /site_type="other"
                     /note="UBA interaction site [polypeptide binding]"
                     /db_xref="CDD:340497"
     Region          1179..1234
                     /region_name="mRING-HC-C3HC3D_RBR_HOIL1"
                     /note="Modified RING finger, HC subclass (C3HC3D-type),
                     found in heme-oxidized IRP2 ubiquitin ligase 1 (HOIL-1)
                     and similar proteins; cd16633"
                     /db_xref="CDD:438295"
     Region          1267..1330
                     /region_name="BRcat_RBR_HOIL1"
                     /note="BRcat domain found in heme-oxidized IRP2 ubiquitin
                     ligase 1 (HOIL-1) and similar proteins; cd20345"
                     /db_xref="CDD:439006"
     Region          1344..1416
                     /region_name="Rcat_RBR_HOIL1"
                     /note="Rcat domain found in heme-oxidized IRP2 ubiquitin
                     ligase 1 (HOIL-1) and similar proteins; cd20358"
                     /db_xref="CDD:439019"
     CDS             1..1416
                     /gene="LOC144113530"
                     /coded_by="XM_077646647.1:233..4483"
                     /db_xref="GeneID:144113530"
ORIGIN      
        1 mslrrpfsed dtgplltlgd rvvwlfehgp ehgwvrwigr ipvvcpswti gvefdnpiga
       61 gdgkfggkry fyarndhayf lpnssllras dynscfrkqn tmggptpspq skmytfphqf
      121 saakcpfpgs anaveathlt aahlissgsn skelgicsag kllrsqstca qdghnlsdyd
      181 nwlggqdpcn vhvkswksss thsvvscppv llpkmlvare ptsssdsemn fgslrslmsc
      241 ftlgshrkkk rrkkkkknhr lvivtgpans nccysggnia saqaktaahl cdgehsikhg
      301 akeldakels cvpsrskamw ryslqeqdmq rreasskepv ashsaeqrmp pklppktstk
      361 kkrlapqppd gtvtpvgtlp rnspvavpsg ssahgtdsls ltrrrshkkp apqppgcdmw
      421 hstarsgtlp sgiqkgvyan enteenkgma tlkakplpvd dvgmcgssll qpyslkcqln
      481 vqenvlnpqe kqdfgknlvs lvikpdlpvk tipspklnmg ndkvattgas ssiqnkdlnd
      541 hqgakafgev gkptlaktvv tenekrdayk hqafdrelrs stlpstrqcs pileeeepya
      601 ttavcgefnn lesvlkrnsn vfelldafsk lelnsdetpk dsepespkhq kkqvkslksl
      661 fqkhpadask vvstkwtytp meakkenlfa atkptfpiat rpgdygntyt kventdpssl
      721 csethstkks psqlplntpl ascrmnlkpv shspdfgnvf cklrsqasgs sgnppwknql
      781 qalkampdep rpstselpvv etknkpsggr kegwgslvge sascsvvaat agfplevmag
      841 kaspasspvp sslssahinp grqrppahpn sptalmqakl palfrqleea iskgehdraa
      901 ilarelamyk vscslrrvrk vapdpkqftl kmyvedrksh vgpislpvyp emtlsnlkkk
      961 veaeynipvr vqrwilgknl atddeatleq fgvsqgacpv flylvspaee lkaeevlrns
     1021 swkppdsvas stdevdelap talrqapsda sstldallnd qaagwtcqlc ginntepdtc
     1081 amcgvtalad aidhstvdas hrtavdtdpd aepilrhdle pieihanenn qlgvdndpve
     1141 vdfpqgqqdg tnaevpkqpp qpllpqdymr lveldgqdlv psmeafecsv cflgvepgqg
     1201 vllrdclhmf crdclsnsvr yaeeamvkcp frndeyscss hlqereiral vspevyehhl
     1261 srsvktaesq apnsfhckta dcpgwcmled nvnvflcpvc ghsncltcra ihegknclqy
     1321 qdeldfnapn gqeakqtkey ldnmvkegqa mhcpqcrviv mkkwgcdwlk csvcqteicw
     1381 vtkgprwgpq gkgdtsagck cgvngvkchp kcnych
//
LOCUS       XP_077502774            1416 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113530 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502774
VERSION     XP_077502774.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646648.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1416
                     /product="uncharacterized protein LOC144113530 isoform X1"
                     /calculated_mol_wt=154850
     Region          18..82
                     /region_name="CAP_GLY"
                     /note="CAP-Gly domain; pfam01302"
                     /db_xref="CDD:460154"
     Region          927..1007
                     /region_name="Ubl_HOIL1"
                     /note="ubiquitin-like (Ubl) domain found in heme-oxidized
                     IRP2 ubiquitin ligase 1 (HOIL-1) and similar proteins;
                     cd01799"
                     /db_xref="CDD:340497"
     Site            order(929,934..936,938..940,942..945,964..965,967..968,
                     1005..1007)
                     /site_type="other"
                     /note="UBA interaction site [polypeptide binding]"
                     /db_xref="CDD:340497"
     Region          1179..1234
                     /region_name="mRING-HC-C3HC3D_RBR_HOIL1"
                     /note="Modified RING finger, HC subclass (C3HC3D-type),
                     found in heme-oxidized IRP2 ubiquitin ligase 1 (HOIL-1)
                     and similar proteins; cd16633"
                     /db_xref="CDD:438295"
     Region          1267..1330
                     /region_name="BRcat_RBR_HOIL1"
                     /note="BRcat domain found in heme-oxidized IRP2 ubiquitin
                     ligase 1 (HOIL-1) and similar proteins; cd20345"
                     /db_xref="CDD:439006"
     Region          1344..1416
                     /region_name="Rcat_RBR_HOIL1"
                     /note="Rcat domain found in heme-oxidized IRP2 ubiquitin
                     ligase 1 (HOIL-1) and similar proteins; cd20358"
                     /db_xref="CDD:439019"
     CDS             1..1416
                     /gene="LOC144113530"
                     /coded_by="XM_077646648.1:187..4437"
                     /db_xref="GeneID:144113530"
ORIGIN      
        1 mslrrpfsed dtgplltlgd rvvwlfehgp ehgwvrwigr ipvvcpswti gvefdnpiga
       61 gdgkfggkry fyarndhayf lpnssllras dynscfrkqn tmggptpspq skmytfphqf
      121 saakcpfpgs anaveathlt aahlissgsn skelgicsag kllrsqstca qdghnlsdyd
      181 nwlggqdpcn vhvkswksss thsvvscppv llpkmlvare ptsssdsemn fgslrslmsc
      241 ftlgshrkkk rrkkkkknhr lvivtgpans nccysggnia saqaktaahl cdgehsikhg
      301 akeldakels cvpsrskamw ryslqeqdmq rreasskepv ashsaeqrmp pklppktstk
      361 kkrlapqppd gtvtpvgtlp rnspvavpsg ssahgtdsls ltrrrshkkp apqppgcdmw
      421 hstarsgtlp sgiqkgvyan enteenkgma tlkakplpvd dvgmcgssll qpyslkcqln
      481 vqenvlnpqe kqdfgknlvs lvikpdlpvk tipspklnmg ndkvattgas ssiqnkdlnd
      541 hqgakafgev gkptlaktvv tenekrdayk hqafdrelrs stlpstrqcs pileeeepya
      601 ttavcgefnn lesvlkrnsn vfelldafsk lelnsdetpk dsepespkhq kkqvkslksl
      661 fqkhpadask vvstkwtytp meakkenlfa atkptfpiat rpgdygntyt kventdpssl
      721 csethstkks psqlplntpl ascrmnlkpv shspdfgnvf cklrsqasgs sgnppwknql
      781 qalkampdep rpstselpvv etknkpsggr kegwgslvge sascsvvaat agfplevmag
      841 kaspasspvp sslssahinp grqrppahpn sptalmqakl palfrqleea iskgehdraa
      901 ilarelamyk vscslrrvrk vapdpkqftl kmyvedrksh vgpislpvyp emtlsnlkkk
      961 veaeynipvr vqrwilgknl atddeatleq fgvsqgacpv flylvspaee lkaeevlrns
     1021 swkppdsvas stdevdelap talrqapsda sstldallnd qaagwtcqlc ginntepdtc
     1081 amcgvtalad aidhstvdas hrtavdtdpd aepilrhdle pieihanenn qlgvdndpve
     1141 vdfpqgqqdg tnaevpkqpp qpllpqdymr lveldgqdlv psmeafecsv cflgvepgqg
     1201 vllrdclhmf crdclsnsvr yaeeamvkcp frndeyscss hlqereiral vspevyehhl
     1261 srsvktaesq apnsfhckta dcpgwcmled nvnvflcpvc ghsncltcra ihegknclqy
     1321 qdeldfnapn gqeakqtkey ldnmvkegqa mhcpqcrviv mkkwgcdwlk csvcqteicw
     1381 vtkgprwgpq gkgdtsagck cgvngvkchp kcnych
//
LOCUS       XP_077502775            1416 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113530 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502775
VERSION     XP_077502775.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646649.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1416
                     /product="uncharacterized protein LOC144113530 isoform X1"
                     /calculated_mol_wt=154850
     Region          18..82
                     /region_name="CAP_GLY"
                     /note="CAP-Gly domain; pfam01302"
                     /db_xref="CDD:460154"
     Region          927..1007
                     /region_name="Ubl_HOIL1"
                     /note="ubiquitin-like (Ubl) domain found in heme-oxidized
                     IRP2 ubiquitin ligase 1 (HOIL-1) and similar proteins;
                     cd01799"
                     /db_xref="CDD:340497"
     Site            order(929,934..936,938..940,942..945,964..965,967..968,
                     1005..1007)
                     /site_type="other"
                     /note="UBA interaction site [polypeptide binding]"
                     /db_xref="CDD:340497"
     Region          1179..1234
                     /region_name="mRING-HC-C3HC3D_RBR_HOIL1"
                     /note="Modified RING finger, HC subclass (C3HC3D-type),
                     found in heme-oxidized IRP2 ubiquitin ligase 1 (HOIL-1)
                     and similar proteins; cd16633"
                     /db_xref="CDD:438295"
     Region          1267..1330
                     /region_name="BRcat_RBR_HOIL1"
                     /note="BRcat domain found in heme-oxidized IRP2 ubiquitin
                     ligase 1 (HOIL-1) and similar proteins; cd20345"
                     /db_xref="CDD:439006"
     Region          1344..1416
                     /region_name="Rcat_RBR_HOIL1"
                     /note="Rcat domain found in heme-oxidized IRP2 ubiquitin
                     ligase 1 (HOIL-1) and similar proteins; cd20358"
                     /db_xref="CDD:439019"
     CDS             1..1416
                     /gene="LOC144113530"
                     /coded_by="XM_077646649.1:104..4354"
                     /db_xref="GeneID:144113530"
ORIGIN      
        1 mslrrpfsed dtgplltlgd rvvwlfehgp ehgwvrwigr ipvvcpswti gvefdnpiga
       61 gdgkfggkry fyarndhayf lpnssllras dynscfrkqn tmggptpspq skmytfphqf
      121 saakcpfpgs anaveathlt aahlissgsn skelgicsag kllrsqstca qdghnlsdyd
      181 nwlggqdpcn vhvkswksss thsvvscppv llpkmlvare ptsssdsemn fgslrslmsc
      241 ftlgshrkkk rrkkkkknhr lvivtgpans nccysggnia saqaktaahl cdgehsikhg
      301 akeldakels cvpsrskamw ryslqeqdmq rreasskepv ashsaeqrmp pklppktstk
      361 kkrlapqppd gtvtpvgtlp rnspvavpsg ssahgtdsls ltrrrshkkp apqppgcdmw
      421 hstarsgtlp sgiqkgvyan enteenkgma tlkakplpvd dvgmcgssll qpyslkcqln
      481 vqenvlnpqe kqdfgknlvs lvikpdlpvk tipspklnmg ndkvattgas ssiqnkdlnd
      541 hqgakafgev gkptlaktvv tenekrdayk hqafdrelrs stlpstrqcs pileeeepya
      601 ttavcgefnn lesvlkrnsn vfelldafsk lelnsdetpk dsepespkhq kkqvkslksl
      661 fqkhpadask vvstkwtytp meakkenlfa atkptfpiat rpgdygntyt kventdpssl
      721 csethstkks psqlplntpl ascrmnlkpv shspdfgnvf cklrsqasgs sgnppwknql
      781 qalkampdep rpstselpvv etknkpsggr kegwgslvge sascsvvaat agfplevmag
      841 kaspasspvp sslssahinp grqrppahpn sptalmqakl palfrqleea iskgehdraa
      901 ilarelamyk vscslrrvrk vapdpkqftl kmyvedrksh vgpislpvyp emtlsnlkkk
      961 veaeynipvr vqrwilgknl atddeatleq fgvsqgacpv flylvspaee lkaeevlrns
     1021 swkppdsvas stdevdelap talrqapsda sstldallnd qaagwtcqlc ginntepdtc
     1081 amcgvtalad aidhstvdas hrtavdtdpd aepilrhdle pieihanenn qlgvdndpve
     1141 vdfpqgqqdg tnaevpkqpp qpllpqdymr lveldgqdlv psmeafecsv cflgvepgqg
     1201 vllrdclhmf crdclsnsvr yaeeamvkcp frndeyscss hlqereiral vspevyehhl
     1261 srsvktaesq apnsfhckta dcpgwcmled nvnvflcpvc ghsncltcra ihegknclqy
     1321 qdeldfnapn gqeakqtkey ldnmvkegqa mhcpqcrviv mkkwgcdwlk csvcqteicw
     1381 vtkgprwgpq gkgdtsagck cgvngvkchp kcnych
//
LOCUS       XP_077502776            1415 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113530 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502776
VERSION     XP_077502776.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646650.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1415
                     /product="uncharacterized protein LOC144113530 isoform X2"
                     /calculated_mol_wt=154722
     Region          18..82
                     /region_name="CAP_GLY"
                     /note="CAP-Gly domain; pfam01302"
                     /db_xref="CDD:460154"
     Region          927..1007
                     /region_name="Ubl_HOIL1"
                     /note="ubiquitin-like (Ubl) domain found in heme-oxidized
                     IRP2 ubiquitin ligase 1 (HOIL-1) and similar proteins;
                     cd01799"
                     /db_xref="CDD:340497"
     Site            order(929,934..936,938..940,942..945,964..965,967..968,
                     1005..1007)
                     /site_type="other"
                     /note="UBA interaction site [polypeptide binding]"
                     /db_xref="CDD:340497"
     Region          1178..1233
                     /region_name="mRING-HC-C3HC3D_RBR_HOIL1"
                     /note="Modified RING finger, HC subclass (C3HC3D-type),
                     found in heme-oxidized IRP2 ubiquitin ligase 1 (HOIL-1)
                     and similar proteins; cd16633"
                     /db_xref="CDD:438295"
     Region          1266..1329
                     /region_name="BRcat_RBR_HOIL1"
                     /note="BRcat domain found in heme-oxidized IRP2 ubiquitin
                     ligase 1 (HOIL-1) and similar proteins; cd20345"
                     /db_xref="CDD:439006"
     Region          1343..1415
                     /region_name="Rcat_RBR_HOIL1"
                     /note="Rcat domain found in heme-oxidized IRP2 ubiquitin
                     ligase 1 (HOIL-1) and similar proteins; cd20358"
                     /db_xref="CDD:439019"
     CDS             1..1415
                     /gene="LOC144113530"
                     /coded_by="XM_077646650.1:535..4782"
                     /db_xref="GeneID:144113530"
ORIGIN      
        1 mslrrpfsed dtgplltlgd rvvwlfehgp ehgwvrwigr ipvvcpswti gvefdnpiga
       61 gdgkfggkry fyarndhayf lpnssllras dynscfrkqn tmggptpspq skmytfphqf
      121 saakcpfpgs anaveathlt aahlissgsn skelgicsag kllrsqstca qdghnlsdyd
      181 nwlggqdpcn vhvkswksss thsvvscppv llpkmlvare ptsssdsemn fgslrslmsc
      241 ftlgshrkkk rrkkkkknhr lvivtgpans nccysggnia saqaktaahl cdgehsikhg
      301 akeldakels cvpsrskamw ryslqeqdmq rreasskepv ashsaeqrmp pklppktstk
      361 kkrlapqppd gtvtpvgtlp rnspvavpsg ssahgtdsls ltrrrshkkp apqppgcdmw
      421 hstarsgtlp sgiqkgvyan enteenkgma tlkakplpvd dvgmcgssll qpyslkcqln
      481 vqenvlnpqe kqdfgknlvs lvikpdlpvk tipspklnmg ndkvattgas ssiqnkdlnd
      541 hqgakafgev gkptlaktvv tenekrdayk hqafdrelrs stlpstrqcs pileeeepya
      601 ttavcgefnn lesvlkrnsn vfelldafsk lelnsdetpk dsepespkhq kkqvkslksl
      661 fqkhpadask vvstkwtytp meakkenlfa atkptfpiat rpgdygntyt kventdpssl
      721 csethstkks psqlplntpl ascrmnlkpv shspdfgnvf cklrsqasgs sgnppwknql
      781 qalkampdep rpstselpvv etknkpsggr kegwgslvge sascsvvaat agfplevmag
      841 kaspasspvp sslssahinp grqrppahpn sptalmqakl palfrqleea iskgehdraa
      901 ilarelamyk vscslrrvrk vapdpkqftl kmyvedrksh vgpislpvyp emtlsnlkkk
      961 veaeynipvr vqrwilgknl atddeatleq fgvsqgacpv flylvspaee lkaeevlrns
     1021 swkppdsvas stdevdelap talrqapsda sstldallnd qaagwtcqlc ginntepdtc
     1081 amcgvtalad aidhstvdas hrtavdtdpd aepilrhdle pieihanenn qlgvdndpve
     1141 vdfpqgqdgt naevpkqppq pllpqdymrl veldgqdlvp smeafecsvc flgvepgqgv
     1201 llrdclhmfc rdclsnsvry aeeamvkcpf rndeyscssh lqereiralv spevyehhls
     1261 rsvktaesqa pnsfhcktad cpgwcmledn vnvflcpvcg hsncltcrai hegknclqyq
     1321 deldfnapng qeakqtkeyl dnmvkegqam hcpqcrvivm kkwgcdwlkc svcqteicwv
     1381 tkgprwgpqg kgdtsagckc gvngvkchpk cnych
//
LOCUS       XP_077502777            1299 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113530 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502777
VERSION     XP_077502777.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646651.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1299
                     /product="uncharacterized protein LOC144113530 isoform X3"
                     /calculated_mol_wt=141642
     Region          810..890
                     /region_name="Ubl_HOIL1"
                     /note="ubiquitin-like (Ubl) domain found in heme-oxidized
                     IRP2 ubiquitin ligase 1 (HOIL-1) and similar proteins;
                     cd01799"
                     /db_xref="CDD:340497"
     Site            order(812,817..819,821..823,825..828,847..848,850..851,
                     888..890)
                     /site_type="other"
                     /note="UBA interaction site [polypeptide binding]"
                     /db_xref="CDD:340497"
     Region          1062..1117
                     /region_name="mRING-HC-C3HC3D_RBR_HOIL1"
                     /note="Modified RING finger, HC subclass (C3HC3D-type),
                     found in heme-oxidized IRP2 ubiquitin ligase 1 (HOIL-1)
                     and similar proteins; cd16633"
                     /db_xref="CDD:438295"
     Region          1150..1213
                     /region_name="BRcat_RBR_HOIL1"
                     /note="BRcat domain found in heme-oxidized IRP2 ubiquitin
                     ligase 1 (HOIL-1) and similar proteins; cd20345"
                     /db_xref="CDD:439006"
     Region          1227..1299
                     /region_name="Rcat_RBR_HOIL1"
                     /note="Rcat domain found in heme-oxidized IRP2 ubiquitin
                     ligase 1 (HOIL-1) and similar proteins; cd20358"
                     /db_xref="CDD:439019"
     CDS             1..1299
                     /gene="LOC144113530"
                     /coded_by="XM_077646651.1:688..4587"
                     /db_xref="GeneID:144113530"
ORIGIN      
        1 mvfeitpnck pgsanaveat hltaahliss gsnskelgic sagkllrsqs tcaqdghnls
       61 dydnwlggqd pcnvhvkswk sssthsvvsc ppvllpkmlv areptsssds emnfgslrsl
      121 mscftlgshr kkkrrkkkkk nhrlvivtgp ansnccysgg niasaqakta ahlcdgehsi
      181 khgakeldak elscvpsrsk amwryslqeq dmqrreassk epvashsaeq rmppklppkt
      241 stkkkrlapq ppdgtvtpvg tlprnspvav psgssahgtd slsltrrrsh kkpapqppgc
      301 dmwhstarsg tlpsgiqkgv yanenteenk gmatlkakpl pvddvgmcgs sllqpyslkc
      361 qlnvqenvln pqekqdfgkn lvslvikpdl pvktipspkl nmgndkvatt gasssiqnkd
      421 lndhqgakaf gevgkptlak tvvtenekrd aykhqafdre lrsstlpstr qcspileeee
      481 pyattavcge fnnlesvlkr nsnvfellda fsklelnsde tpkdsepesp khqkkqvksl
      541 kslfqkhpad askvvstkwt ytpmeakken lfaatkptfp iatrpgdygn tytkventdp
      601 sslcsethst kkspsqlpln tplascrmnl kpvshspdfg nvfcklrsqa sgssgnppwk
      661 nqlqalkamp deprpstsel pvvetknkps ggrkegwgsl vgesascsvv aatagfplev
      721 magkaspass pvpsslssah inpgrqrppa hpnsptalmq aklpalfrql eeaiskgehd
      781 raailarela mykvscslrr vrkvapdpkq ftlkmyvedr kshvgpislp vypemtlsnl
      841 kkkveaeyni pvrvqrwilg knlatddeat leqfgvsqga cpvflylvsp aeelkaeevl
      901 rnsswkppds vasstdevde laptalrqap sdasstldal lndqaagwtc qlcginntep
      961 dtcamcgvta ladaidhstv dashrtavdt dpdaepilrh dlepieihan ennqlgvdnd
     1021 pvevdfpqgq qdgtnaevpk qppqpllpqd ymrlveldgq dlvpsmeafe csvcflgvep
     1081 gqgvllrdcl hmfcrdclsn svryaeeamv kcpfrndeys csshlqerei ralvspevye
     1141 hhlsrsvkta esqapnsfhc ktadcpgwcm lednvnvflc pvcghsnclt craihegknc
     1201 lqyqdeldfn apngqeakqt keyldnmvke gqamhcpqcr vivmkkwgcd wlkcsvcqte
     1261 icwvtkgprw gpqgkgdtsa gckcgvngvk chpkcnych
//
LOCUS       XP_077502778            1298 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113530 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077502778
VERSION     XP_077502778.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646652.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1298
                     /product="uncharacterized protein LOC144113530 isoform X4"
                     /calculated_mol_wt=141514
     Region          810..890
                     /region_name="Ubl_HOIL1"
                     /note="ubiquitin-like (Ubl) domain found in heme-oxidized
                     IRP2 ubiquitin ligase 1 (HOIL-1) and similar proteins;
                     cd01799"
                     /db_xref="CDD:340497"
     Site            order(812,817..819,821..823,825..828,847..848,850..851,
                     888..890)
                     /site_type="other"
                     /note="UBA interaction site [polypeptide binding]"
                     /db_xref="CDD:340497"
     Region          1061..1116
                     /region_name="mRING-HC-C3HC3D_RBR_HOIL1"
                     /note="Modified RING finger, HC subclass (C3HC3D-type),
                     found in heme-oxidized IRP2 ubiquitin ligase 1 (HOIL-1)
                     and similar proteins; cd16633"
                     /db_xref="CDD:438295"
     Region          1149..1212
                     /region_name="BRcat_RBR_HOIL1"
                     /note="BRcat domain found in heme-oxidized IRP2 ubiquitin
                     ligase 1 (HOIL-1) and similar proteins; cd20345"
                     /db_xref="CDD:439006"
     Region          1226..1298
                     /region_name="Rcat_RBR_HOIL1"
                     /note="Rcat domain found in heme-oxidized IRP2 ubiquitin
                     ligase 1 (HOIL-1) and similar proteins; cd20358"
                     /db_xref="CDD:439019"
     CDS             1..1298
                     /gene="LOC144113530"
                     /coded_by="XM_077646652.1:828..4724"
                     /db_xref="GeneID:144113530"
ORIGIN      
        1 mvfeitpnck pgsanaveat hltaahliss gsnskelgic sagkllrsqs tcaqdghnls
       61 dydnwlggqd pcnvhvkswk sssthsvvsc ppvllpkmlv areptsssds emnfgslrsl
      121 mscftlgshr kkkrrkkkkk nhrlvivtgp ansnccysgg niasaqakta ahlcdgehsi
      181 khgakeldak elscvpsrsk amwryslqeq dmqrreassk epvashsaeq rmppklppkt
      241 stkkkrlapq ppdgtvtpvg tlprnspvav psgssahgtd slsltrrrsh kkpapqppgc
      301 dmwhstarsg tlpsgiqkgv yanenteenk gmatlkakpl pvddvgmcgs sllqpyslkc
      361 qlnvqenvln pqekqdfgkn lvslvikpdl pvktipspkl nmgndkvatt gasssiqnkd
      421 lndhqgakaf gevgkptlak tvvtenekrd aykhqafdre lrsstlpstr qcspileeee
      481 pyattavcge fnnlesvlkr nsnvfellda fsklelnsde tpkdsepesp khqkkqvksl
      541 kslfqkhpad askvvstkwt ytpmeakken lfaatkptfp iatrpgdygn tytkventdp
      601 sslcsethst kkspsqlpln tplascrmnl kpvshspdfg nvfcklrsqa sgssgnppwk
      661 nqlqalkamp deprpstsel pvvetknkps ggrkegwgsl vgesascsvv aatagfplev
      721 magkaspass pvpsslssah inpgrqrppa hpnsptalmq aklpalfrql eeaiskgehd
      781 raailarela mykvscslrr vrkvapdpkq ftlkmyvedr kshvgpislp vypemtlsnl
      841 kkkveaeyni pvrvqrwilg knlatddeat leqfgvsqga cpvflylvsp aeelkaeevl
      901 rnsswkppds vasstdevde laptalrqap sdasstldal lndqaagwtc qlcginntep
      961 dtcamcgvta ladaidhstv dashrtavdt dpdaepilrh dlepieihan ennqlgvdnd
     1021 pvevdfpqgq dgtnaevpkq ppqpllpqdy mrlveldgqd lvpsmeafec svcflgvepg
     1081 qgvllrdclh mfcrdclsns vryaeeamvk cpfrndeysc sshlqereir alvspevyeh
     1141 hlsrsvktae sqapnsfhck tadcpgwcml ednvnvflcp vcghsncltc raihegkncl
     1201 qyqdeldfna pngqeakqtk eyldnmvkeg qamhcpqcrv ivmkkwgcdw lkcsvcqtei
     1261 cwvtkgprwg pqgkgdtsag ckcgvngvkc hpkcnych
//
LOCUS       XP_077502779             570 aa            linear   INV 25-NOV-2025
DEFINITION  ran-binding protein M isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502779
VERSION     XP_077502779.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646653.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..570
                     /product="ran-binding protein M isoform X1"
                     /calculated_mol_wt=62360
     Region          79..217
                     /region_name="SPRY_RanBP9_10"
                     /note="SPRY domain in Ran binding proteins 9 and 10;
                     cd12909"
                     /db_xref="CDD:293966"
     Region          250..282
                     /region_name="LisH"
                     /note="Lissencephaly type-1-like homology motif;
                     smart00667"
                     /db_xref="CDD:128913"
     Region          289..550
                     /region_name="CTLH"
                     /note="CTLH/CRA C-terminal to LisH motif domain;
                     pfam10607"
                     /db_xref="CDD:402305"
     CDS             1..570
                     /gene="RanBPM"
                     /coded_by="XM_077646653.1:37..1749"
                     /db_xref="GeneID:144113532"
ORIGIN      
        1 matmnqafgg tesatsysqq ptdrlkllyp avneeetplp rswspkdkft yiglsqnnlr
       61 vhykgnafst amcsstrigk nhkdaasvra thpipaacgl yyfevkivsk grdgymgvgl
      121 saqgvnmnrl pgwdkhsygy hgddghsfcs sgtgqpygpt fttgdvigcg vnlidnlcfy
      181 tknghnlgia ftdlppnlyp tvglqtpgev vdanfgqspf vfdiedmmke lmmrtrttie
      241 rfpvndkqge wqttlhrvvq tylvhhgyca taeafarstg qsfdedlssi knrqriqklv
      301 lagrvgeaie ttqrlypgll ennpnllfll rcrqfvemvn gtdsevrpar rspgssprll
      361 llssspkstg sdvasngvaa sdgsaangda tlngdhnddd cgtdmvvegs egrtmvtngl
      421 dpdsrqhngh fhrldlgdtd mdvdppkrql cggnqaamer mlqfgrelhl msvqlkhqfg
      481 tnkanrkmiq dafsllayad pwsspigyql dpvqrepvca alnsailesq klprrpplel
      541 ivahsrellk lmskaglgsc afanidsllq
//
LOCUS       XP_077502780             558 aa            linear   INV 25-NOV-2025
DEFINITION  ran-binding protein M isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502780
VERSION     XP_077502780.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646654.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..558
                     /product="ran-binding protein M isoform X2"
                     /calculated_mol_wt=61103
     Region          63..205
                     /region_name="SPRY_RanBP9_10"
                     /note="SPRY domain in Ran binding proteins 9 and 10;
                     cd12909"
                     /db_xref="CDD:293966"
     Region          238..270
                     /region_name="LisH"
                     /note="Lissencephaly type-1-like homology motif;
                     smart00667"
                     /db_xref="CDD:128913"
     Region          277..538
                     /region_name="CTLH"
                     /note="CTLH/CRA C-terminal to LisH motif domain;
                     pfam10607"
                     /db_xref="CDD:402305"
     CDS             1..558
                     /gene="RanBPM"
                     /coded_by="XM_077646654.1:35..1711"
                     /db_xref="GeneID:144113532"
ORIGIN      
        1 matmnqafgg tesatsysqq ptdrlkllyp avneeetplp rswspkdkft yiglsqnnlr
       61 vhykgigknh kdaasvrath pipaacglyy fevkivskgr dgymgvglsa qgvnmnrlpg
      121 wdkhsygyhg ddghsfcssg tgqpygptft tgdvigcgvn lidnlcfytk nghnlgiaft
      181 dlppnlyptv glqtpgevvd anfgqspfvf diedmmkelm mrtrttierf pvndkqgewq
      241 ttlhrvvqty lvhhgycata eafarstgqs fdedlssikn rqriqklvla grvgeaiett
      301 qrlypgllen npnllfllrc rqfvemvngt dsevrparrs pgssprllll ssspkstgsd
      361 vasngvaasd gsaangdatl ngdhndddcg tdmvvegseg rtmvtngldp dsrqhnghfh
      421 rldlgdtdmd vdppkrqlcg gnqaamerml qfgrelhlms vqlkhqfgtn kanrkmiqda
      481 fsllayadpw sspigyqldp vqrepvcaal nsailesqkl prrppleliv ahsrellklm
      541 skaglgscaf anidsllq
//
LOCUS       XP_077502781             438 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial ribosomal protein L38 [Amblyomma americanum].
ACCESSION   XP_077502781
VERSION     XP_077502781.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646655.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..438
                     /product="mitochondrial ribosomal protein L38"
                     /calculated_mol_wt=51868
     Region          152..288
                     /region_name="PEBP_euk"
                     /note="PhosphatidylEthanolamine-Binding Protein (PEBP)
                     domain present in eukaryotes; cd00866"
                     /db_xref="CDD:176644"
     Site            order(187,199,201,224..227,233,235)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:176644"
     CDS             1..438
                     /gene="mRpL38"
                     /coded_by="XM_077646655.1:161..1477"
                     /db_xref="GeneID:144113534"
ORIGIN      
        1 matlaaflkn fralrrlepa marcvrlppm dtlksleekm qalkakepvf eervnigfkv
       61 aphsvnkqew krqrkiiqen kdnydlaeks rngtlkiqce karqewrkty apmhvkevad
      121 hygvfrdlye fgffhpvipv dvlyeydaey ftpvhygnii lpseaakapt vlfdsepnml
      181 wtlvltslds hllendkeyl hwfignikgn qvpsgevvcd ymqpflprgt gyhrfvfvly
      241 kqeglidysn qklsanstsl kertfktydf ykefenvltp aglaffqctw edslvdffhk
      301 tlkmrvptfe yvhppeyvpk qvlyphkqpf ntyldmyrdp rdireevylk rlkslnpled
      361 eppmpkypni ykipqgtpsw lvdemrkerf rigkykdlrp fsiypeeeye dpkvkawreq
      421 keferkwaeq kaakssps
//
LOCUS       XP_077502782             591 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113535 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502782
VERSION     XP_077502782.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646656.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..591
                     /product="uncharacterized protein LOC144113535 isoform X1"
                     /calculated_mol_wt=62782
     Region          1..269
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(21..23,25..26,30,33,42,44,46,50..51,54..56,58..59,
                     63,66,75,77,79,83..84,87..89,91..92,96,99)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          44..75
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          77..106
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          110..135
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          149..177
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          181..211
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          196..>372
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          213..250
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          282..312
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(283,287..288,291..293,295..296,300,303,312,314,317,
                     321..322,325..327,329..330,334,337,346,348,350,354..355,
                     360..362,364..365,369)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          314..346
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          522..584
                     /region_name="SAM_superfamily"
                     /note="SAM (Sterile alpha motif); cl15755"
                     /db_xref="CDD:472832"
     CDS             1..591
                     /gene="LOC144113535"
                     /coded_by="XM_077646656.1:81..1856"
                     /db_xref="GeneID:144113535"
ORIGIN      
        1 mqtmnnsesm ekvasflslc lendlpavrs sltnraavda qdaegttglq iaatngykil
       61 aeyligqsad vnlannygwt plmhaaqhgq vsvvalllhh gatvnatgrl gvgalalaag
      121 gghhsavcll leagadtads avhcgsvltp lgaaavnghv svigallqrg ssvnqvllat
      181 gasplmlaas gghlraaqvl mhhranpnqn nfygqtaldv atacakmeva eylrsiaqva
      241 qqehnpnvtd ifdaivacdl nevrrileeh ppccnwchsk kgttplmqaa ilgyidaval
      301 lvesnadlem qdcsagwtal mhavyhgqlh varylissga dvyteafnga taldiacyle
      361 ngntkiiqml adetvaglss qlaaeterkt ndsqhlkvlk ewigsvtqkl ttwrgrqpll
      421 tsigwaaslp sslavdlqsm ststthetvp aldisftspe tifpevdsvf waappsltny
      481 kkmlegvqaa lpdsaigths qkltrraiep kfhhlkqppl tesessggvm silqalglgk
      541 yarsfeenev dweafvnldp gsieaigvlt gisrevlqea iyelqkmrpa c
//
LOCUS       XP_077502783             529 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113535 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502783
VERSION     XP_077502783.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646657.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..529
                     /product="uncharacterized protein LOC144113535 isoform X2"
                     /calculated_mol_wt=56596
     Region          86..310
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          87..115
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          119..149
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          151..188
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          220..250
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(221,225..226,229..231,233..234,238,241,250,252,255,
                     259..260,263..265,267..268,272,275,284,286,288,292..293,
                     298..300,302..303,307)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          252..284
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          460..522
                     /region_name="SAM_superfamily"
                     /note="SAM (Sterile alpha motif); cl15755"
                     /db_xref="CDD:472832"
     CDS             1..529
                     /gene="LOC144113535"
                     /coded_by="XM_077646657.1:81..1670"
                     /db_xref="GeneID:144113535"
ORIGIN      
        1 mqtmnnsesm ekvasflslc lendlpaelp ltpkmqkvqq dcklqprtgv galalaaggg
       61 hhsavcllle agadtadsav hcgsvltplg aaavnghvsv igallqrgss vnqvllatga
      121 splmlaasgg hlraaqvlmh hranpnqnnf ygqtaldvat acakmevaey lrsiaqvaqq
      181 ehnpnvtdif daivacdlne vrrileehpp ccnwchskkg ttplmqaail gyidavallv
      241 esnadlemqd csagwtalmh avyhgqlhva rylissgadv yteafngata ldiacyleng
      301 ntkiiqmlad etvaglssql aaeterktnd sqhlkvlkew igsvtqkltt wrgrqpllts
      361 igwaaslpss lavdlqsmst stthetvpal disftspeti fpevdsvfwa appsltnykk
      421 mlegvqaalp dsaigthsqk ltrraiepkf hhlkqpplte sessggvmsi lqalglgkya
      481 rsfeenevdw eafvnldpgs ieaigvltgi srevlqeaiy elqkmrpac
//
LOCUS       XP_077502784             521 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113535 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502784
VERSION     XP_077502784.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646658.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..521
                     /product="uncharacterized protein LOC144113535 isoform X3"
                     /calculated_mol_wt=56232
     Region          4..301
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(44,46,50..51,54..56,58..59,63,66,75,77,79,83..84,
                     87..89,91..92,96,99,108,110,112,116..117,120..122,
                     124..125,129,132)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          44..75
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          77..108
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          110..138
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          212..242
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(213,217..218,221..223,225..226,230,233,242,244,247,
                     251..252,255..257,259..260,264,267,276,278,280,284..285,
                     290..292,294..295,299)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          244..276
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          452..514
                     /region_name="SAM_superfamily"
                     /note="SAM (Sterile alpha motif); cl15755"
                     /db_xref="CDD:472832"
     CDS             1..521
                     /gene="LOC144113535"
                     /coded_by="XM_077646658.1:81..1646"
                     /db_xref="GeneID:144113535"
ORIGIN      
        1 mqtmnnsesm ekvasflslc lendlpavrs sltnraavda qdaegttglq iaatngykil
       61 aeyligqsad vnlannygwt plmhaaqhgq vsvvalllhh gatvnatgrl gasplmlaas
      121 gghlraaqvl mhhranpnqn nfygqtaldv atacakmeva eylrsiaqva qqehnpnvtd
      181 ifdaivacdl nevrrileeh ppccnwchsk kgttplmqaa ilgyidaval lvesnadlem
      241 qdcsagwtal mhavyhgqlh varylissga dvyteafnga taldiacyle ngntkiiqml
      301 adetvaglss qlaaeterkt ndsqhlkvlk ewigsvtqkl ttwrgrqpll tsigwaaslp
      361 sslavdlqsm ststthetvp aldisftspe tifpevdsvf waappsltny kkmlegvqaa
      421 lpdsaigths qkltrraiep kfhhlkqppl tesessggvm silqalglgk yarsfeenev
      481 dweafvnldp gsieaigvlt gisrevlqea iyelqkmrpa c
//
LOCUS       XP_077502785             509 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat and SAM domain-containing protein 6-like isoform X4
            [Amblyomma americanum].
ACCESSION   XP_077502785
VERSION     XP_077502785.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646659.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..509
                     /product="ankyrin repeat and SAM domain-containing protein
                     6-like isoform X4"
                     /calculated_mol_wt=54018
     Region          12..290
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          67..95
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          99..129
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          131..168
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          200..230
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(201,205..206,209..211,213..214,218,221,230,232,235,
                     239..240,243..245,247..248,252,255,264,266,268,272..273,
                     278..280,282..283,287)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          232..264
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          440..502
                     /region_name="SAM_superfamily"
                     /note="SAM (Sterile alpha motif); cl15755"
                     /db_xref="CDD:472832"
     CDS             1..509
                     /gene="LOC144113535"
                     /coded_by="XM_077646659.1:305..1834"
                     /db_xref="GeneID:144113535"
ORIGIN      
        1 mhaaqhgqvs vvalllhhga tvnatgrlgv galalaaggg hhsavcllle agadtadsav
       61 hcgsvltplg aaavnghvsv igallqrgss vnqvllatga splmlaasgg hlraaqvlmh
      121 hranpnqnnf ygqtaldvat acakmevaey lrsiaqvaqq ehnpnvtdif daivacdlne
      181 vrrileehpp ccnwchskkg ttplmqaail gyidavallv esnadlemqd csagwtalmh
      241 avyhgqlhva rylissgadv yteafngata ldiacyleng ntkiiqmlad etvaglssql
      301 aaeterktnd sqhlkvlkew igsvtqkltt wrgrqpllts igwaaslpss lavdlqsmst
      361 stthetvpal disftspeti fpevdsvfwa appsltnykk mlegvqaalp dsaigthsqk
      421 ltrraiepkf hhlkqpplte sessggvmsi lqalglgkya rsfeenevdw eafvnldpgs
      481 ieaigvltgi srevlqeaiy elqkmrpac
//
LOCUS       XP_077502786             495 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat and SAM domain-containing protein 6-like isoform X5
            [Amblyomma americanum].
ACCESSION   XP_077502786
VERSION     XP_077502786.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646660.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..495
                     /product="ankyrin repeat and SAM domain-containing protein
                     6-like isoform X5"
                     /calculated_mol_wt=52831
     Region          52..276
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          53..81
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          85..115
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          117..154
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          186..216
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(187,191..192,195..197,199..200,204,207,216,218,221,
                     225..226,229..231,233..234,238,241,250,252,254,258..259,
                     264..266,268..269,273)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          218..250
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          426..488
                     /region_name="SAM_superfamily"
                     /note="SAM (Sterile alpha motif); cl15755"
                     /db_xref="CDD:472832"
     CDS             1..495
                     /gene="LOC144113535"
                     /coded_by="XM_077646660.1:205..1692"
                     /db_xref="GeneID:144113535"
ORIGIN      
        1 mqkvqqdckl qprtgvgala laaggghhsa vcllleagad tadsavhcgs vltplgaaav
       61 nghvsvigal lqrgssvnqv llatgasplm laasgghlra aqvlmhhran pnqnnfygqt
      121 aldvatacak mevaeylrsi aqvaqqehnp nvtdifdaiv acdlnevrri leehppccnw
      181 chskkgttpl mqaailgyid avallvesna dlemqdcsag wtalmhavyh gqlhvaryli
      241 ssgadvytea fngataldia cylengntki iqmladetva glssqlaaet erktndsqhl
      301 kvlkewigsv tqklttwrgr qplltsigwa aslpsslavd lqsmststth etvpaldisf
      361 tspetifpev dsvfwaapps ltnykkmleg vqaalpdsai gthsqkltrr aiepkfhhlk
      421 qppltesess ggvmsilqal glgkyarsfe enevdweafv nldpgsieai gvltgisrev
      481 lqeaiyelqk mrpac
//
LOCUS       XP_077502787             471 aa            linear   INV 25-NOV-2025
DEFINITION  periodic tryptophan protein 1 homolog [Amblyomma americanum].
ACCESSION   XP_077502787
VERSION     XP_077502787.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646661.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..471
                     /product="periodic tryptophan protein 1 homolog"
                     /calculated_mol_wt=51682
     Region          <115..294
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          <221..380
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          225..262
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          269..303
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          311..348
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          344..>422
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          356..393
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          400..424
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..471
                     /gene="LOC144113536"
                     /coded_by="XM_077646661.1:92..1507"
                     /db_xref="GeneID:144113536"
ORIGIN      
        1 mnfvpcltwi prgvakanpq kvkllseqlk eliekgapds knvdgmddme dckaeasedd
       61 itakygldty ddedseapql gnlgslavya nnaddpyldp qhgedseeei ddftirptdn
      121 liavarvded tcatvevyvn nhqeehlyvh hdimlpaypl clewmnfdpt dpnpgnylav
      181 gdmtpvisvw dldlvdslep ayklgkkakk kknavkatmm hkdavlslsw nkqvrhllas
      241 gsadhkalvw dldagvparc lsahkekvqs vvwhpfesht lltgacdstv klwdcrssda
      301 sfkswtvdge vekvlwnhfd pfyfyvstds glvygfdart dqavftlsah tkgvtgmals
      361 aycpgclita sedkslkvwd ildhkpvfvf ekealtvgsv ltlasspdep fvvavggdnk
      421 tlgfavvdlk ewaglscfeg rkllraevqt ddanmetdvp agalgglsld t
//
LOCUS       XP_077502788             275 aa            linear   INV 25-NOV-2025
DEFINITION  AN1-type zinc finger protein 1-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502788
VERSION     XP_077502788.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646662.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..275
                     /product="AN1-type zinc finger protein 1-like isoform X1"
                     /calculated_mol_wt=30964
     Region          15..49
                     /region_name="zf-AN1"
                     /note="AN1-like Zinc finger; pfam01428"
                     /db_xref="CDD:460208"
     Region          73..108
                     /region_name="zf-AN1"
                     /note="AN1-like Zinc finger; pfam01428"
                     /db_xref="CDD:460208"
     CDS             1..275
                     /gene="LOC144113537"
                     /coded_by="XM_077646662.1:243..1070"
                     /db_xref="GeneID:144113537"
ORIGIN      
        1 mskvmaelpq lgkhchvesc nrldflpfec vhckntfcsd hrtsdahvcd rvqthvltdk
       61 earcsrtgtl fccsfgdcsq keltpitcgl cadnfcvrhr haqdhkckcl psqsehmplt
      121 tkvvkkiles kkpvekpiss kaslsptaqk vklmkmkmha ageksvplad rvyfevyfpk
      181 ghkvrskpyh fsklwtvgra idstaiiekl pnqnnrltek rlllfhastl kalgmsdife
      241 tllnskllne aetlvleyvp pncdalaady hyees
//
LOCUS       XP_077502789             241 aa            linear   INV 25-NOV-2025
DEFINITION  AN1-type zinc finger protein 1-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502789
VERSION     XP_077502789.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646663.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..241
                     /product="AN1-type zinc finger protein 1-like isoform X2"
                     /calculated_mol_wt=27200
     Region          39..74
                     /region_name="zf-AN1"
                     /note="AN1-like Zinc finger; pfam01428"
                     /db_xref="CDD:460208"
     CDS             1..241
                     /gene="LOC144113537"
                     /coded_by="XM_077646663.1:299..1024"
                     /db_xref="GeneID:144113537"
ORIGIN      
        1 mqslrfftfr vcalqehilt hvltdkearc srtgtlfccs fgdcsqkelt pitcglcadn
       61 fcvrhrhaqd hkckclpsqs ehmplttkvv kkileskkpv ekpisskasl sptaqkvklm
      121 kmkmhaagek svpladrvyf evyfpkghkv rskpyhfskl wtvgraidst aiieklpnqn
      181 nrltekrlll fhastlkalg msdifetlln skllneaetl vleyvppncd alaadyhyee
      241 s
//
LOCUS       XP_077502790             138 aa            linear   INV 25-NOV-2025
DEFINITION  trafficking protein particle complex subunit 2-like protein
            [Amblyomma americanum].
ACCESSION   XP_077502790
VERSION     XP_077502790.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646664.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..138
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..138
                     /product="trafficking protein particle complex subunit
                     2-like protein"
                     /calculated_mol_wt=15248
     Region          3..136
                     /region_name="TRAPPC2L"
                     /note="Trafficking protein particle complex subunit
                     2-like; cd14854"
                     /db_xref="CDD:341444"
     CDS             1..138
                     /gene="LOC144113539"
                     /coded_by="XM_077646664.1:292..708"
                     /db_xref="GeneID:144113539"
ORIGIN      
        1 mavavavigk ensplfvkta apcnelkfly tihtsldvve ekispgnkss gdvrelylgl
       61 lyptedykvy gyvtntktkf ivivetsrtt lrdneirqmf hklhasycdv vcnpfhvpgd
      121 qivsksfdva vngimtge
//
LOCUS       XP_077502791             138 aa            linear   INV 25-NOV-2025
DEFINITION  trafficking protein particle complex subunit 2-like protein
            [Amblyomma americanum].
ACCESSION   XP_077502791
VERSION     XP_077502791.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646665.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..138
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..138
                     /product="trafficking protein particle complex subunit
                     2-like protein"
                     /calculated_mol_wt=15248
     Region          3..136
                     /region_name="TRAPPC2L"
                     /note="Trafficking protein particle complex subunit
                     2-like; cd14854"
                     /db_xref="CDD:341444"
     CDS             1..138
                     /gene="LOC144113539"
                     /coded_by="XM_077646665.1:283..699"
                     /db_xref="GeneID:144113539"
ORIGIN      
        1 mavavavigk ensplfvkta apcnelkfly tihtsldvve ekispgnkss gdvrelylgl
       61 lyptedykvy gyvtntktkf ivivetsrtt lrdneirqmf hklhasycdv vcnpfhvpgd
      121 qivsksfdva vngimtge
//
LOCUS       XP_077502792             227 aa            linear   INV 25-NOV-2025
DEFINITION  EKC/KEOPS complex subunit TP53RK-like [Amblyomma americanum].
ACCESSION   XP_077502792
VERSION     XP_077502792.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646666.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..227
                     /product="EKC/KEOPS complex subunit TP53RK-like"
                     /calculated_mol_wt=25737
     Region          8..221
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(11..12,15,17,27,35,69,83..86,134,138..139,141,
                     155..156)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..227
                     /gene="LOC144113541"
                     /coded_by="XM_077646666.1:182..865"
                     /db_xref="GeneID:144113541"
ORIGIN      
        1 mdafdavlfk qgaearvykg tyfgraaifk erfekkyrhp dldrllsver lraearalrk
       61 trmagvrvpp vyfvdltsri ivtgyvdnav tvrdrivslq seepatvkes leflmdkige
      121 avallhknhv vhgdlttsnl lvqrrdgesp liyvidfgls fisetaedkg vdlyvleraf
      181 lsahpgiesf fqrflnsysk nysqkaanil kkfgevkqrg rkrtmvg
//
LOCUS       XP_077502793             225 aa            linear   INV 25-NOV-2025
DEFINITION  charged multivesicular body protein shrub [Amblyomma americanum].
ACCESSION   XP_077502793
VERSION     XP_077502793.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646667.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..225
                     /product="charged multivesicular body protein shrub"
                     /calculated_mol_wt=24810
     Region          1..>24
                     /region_name="HlyU"
                     /note="Transcriptional activator HlyU; cl02273"
                     /db_xref="CDD:470516"
     Region          22..157
                     /region_name="Snf7"
                     /note="pfam03357"
                     /db_xref="CDD:460896"
     CDS             1..225
                     /gene="shrb"
                     /coded_by="XM_077646667.1:156..833"
                     /db_xref="GeneID:144113542"
ORIGIN      
        1 msffakmfgg gkggekapsp geaiqrlrei eemlnkkqdf leskvkmele aakkhgtknk
       61 raalaalkrk rryekqlaqi dgtlttieyq realenastn tevlkimsla akalknahen
      121 mdvdkvhdlm devqeqqeia seiseaisnp vafgtdvded elleeletle qqeldeklid
      181 vagpaaedlp evptaepskt paapskpaka heedpemlel akwas
//
LOCUS       XP_077502794             225 aa            linear   INV 25-NOV-2025
DEFINITION  charged multivesicular body protein shrub [Amblyomma americanum].
ACCESSION   XP_077502794
VERSION     XP_077502794.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646668.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..225
                     /product="charged multivesicular body protein shrub"
                     /calculated_mol_wt=24810
     Region          1..>24
                     /region_name="HlyU"
                     /note="Transcriptional activator HlyU; cl02273"
                     /db_xref="CDD:470516"
     Region          22..157
                     /region_name="Snf7"
                     /note="pfam03357"
                     /db_xref="CDD:460896"
     CDS             1..225
                     /gene="shrb"
                     /coded_by="XM_077646668.1:159..836"
                     /db_xref="GeneID:144113542"
ORIGIN      
        1 msffakmfgg gkggekapsp geaiqrlrei eemlnkkqdf leskvkmele aakkhgtknk
       61 raalaalkrk rryekqlaqi dgtlttieyq realenastn tevlkimsla akalknahen
      121 mdvdkvhdlm devqeqqeia seiseaisnp vafgtdvded elleeletle qqeldeklid
      181 vagpaaedlp evptaepskt paapskpaka heedpemlel akwas
//
LOCUS       XP_077502795             225 aa            linear   INV 25-NOV-2025
DEFINITION  charged multivesicular body protein shrub [Amblyomma americanum].
ACCESSION   XP_077502795
VERSION     XP_077502795.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646669.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..225
                     /product="charged multivesicular body protein shrub"
                     /calculated_mol_wt=24810
     Region          1..>24
                     /region_name="HlyU"
                     /note="Transcriptional activator HlyU; cl02273"
                     /db_xref="CDD:470516"
     Region          22..157
                     /region_name="Snf7"
                     /note="pfam03357"
                     /db_xref="CDD:460896"
     CDS             1..225
                     /gene="shrb"
                     /coded_by="XM_077646669.1:167..844"
                     /db_xref="GeneID:144113542"
ORIGIN      
        1 msffakmfgg gkggekapsp geaiqrlrei eemlnkkqdf leskvkmele aakkhgtknk
       61 raalaalkrk rryekqlaqi dgtlttieyq realenastn tevlkimsla akalknahen
      121 mdvdkvhdlm devqeqqeia seiseaisnp vafgtdvded elleeletle qqeldeklid
      181 vagpaaedlp evptaepskt paapskpaka heedpemlel akwas
//
LOCUS       XP_077502796             496 aa            linear   INV 25-NOV-2025
DEFINITION  protein APCDD1-like [Amblyomma americanum].
ACCESSION   XP_077502796
VERSION     XP_077502796.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646670.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..496
                     /product="protein APCDD1-like"
                     /calculated_mol_wt=54922
     Region          47..267
                     /region_name="APCDDC"
                     /note="Adenomatosis polyposis coli down-regulated 1;
                     pfam14921"
                     /db_xref="CDD:464377"
     Region          281..451
                     /region_name="APCDDC"
                     /note="Adenomatosis polyposis coli down-regulated 1;
                     pfam14921"
                     /db_xref="CDD:464377"
     CDS             1..496
                     /gene="LOC144113543"
                     /coded_by="XM_077646670.1:200..1690"
                     /db_xref="GeneID:144113543"
ORIGIN      
        1 mllllllliv gagisgfpgr afakgedgsa rclrwardfa rnwhaetsps epdlsgtwvs
       61 qrcevrsgpe filrkyhffd dskfhmvqff yldssctvpa yaldgwgrle lsslswvvpg
      121 ateaeaslsh lnviaytaev adrlsravnr scpgevkrpw etylkyrlvs fvegrtadkp
      181 liedfvctgg lqftlnelql irivhqgplp nrrqsdapaa elylgdihsd vrkrlsyrpt
      241 syqpplleas aagchvchlv akgaelsppq lppkpklpvh lngewlslrc evqplglfla
      301 rrllfqpgng swsgwfqyyr dpnckqrwfl lsrqgtyela gpsqrlrgat kvnlrtlaaq
      361 ilpqhrgivt nlnsaaedgr cgsrwslrrt qdvtatggcr llgvsvpsta yevahneldv
      421 ygnallflgh adtdgeasrp drratayetp lvqcrslpep snelsgrpla lvsggaavrp
      481 lllcwlplll qlvard
//
LOCUS       XP_077502797             961 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113544 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502797
VERSION     XP_077502797.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646671.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..961
                     /product="uncharacterized protein LOC144113544 isoform X1"
                     /calculated_mol_wt=104995
     Region          219..290
                     /region_name="KH-I_Mextli_like"
                     /note="type I K homology (KH) RNA-binding domain found in
                     Drosophila melanogaster eukaryotic translation initiation
                     factor 4E-binding protein Mextli and similar proteins;
                     cd22454"
                     /db_xref="CDD:411882"
     Site            order(231,233..235,237..241,244..245,248..249,254..257)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411882"
     CDS             1..961
                     /gene="LOC144113544"
                     /coded_by="XM_077646671.1:166..3051"
                     /db_xref="GeneID:144113544"
ORIGIN      
        1 msakntkgvr klerprplrl nsdpekspss vlspsesvtm lidtnaqcav tdedvlkeie
       61 mlhisvnngn ydatilplia nvcdllklkg asmessfkdq ldcyfvtlrn asreerldvv
      121 grlrllevie lramgwkcnq nlteyykrtf aeleghefst ynsssnqmaf lpqlqvqpqq
      181 qqhtmtpiap atspplslqp tevlkssgkf gkpakipgrn flkdeivirn sdsgkvmgik
      241 grrvhmieel sdtvisfqrv npgvrdrlvq itgpseetit rakeliedti rrnaspvrem
      301 gdetsnynmg tkescqkpsl vhsysmgdas mgeytstitv grdvlrisga kavliktakl
      361 vldkyfsgqa efsdkvpdgl spsnqgsyms pepgvlsnnr gttdlcteta swqglavrrh
      421 sgppssqtss ssdeetyked geqelmekap tqspkhvsrs gsivknlpaq iatkasgive
      481 apaqtkqfkr sgsspefsak adrpqvpsta sldssdlpdd aqsnrrmysr efllecsqss
      541 vatqmppdfp sldpevanlm vkqekaptqs pkhvsrsgsi mknlpaqiat kasgiveapa
      601 qtkqfkrsgs spefsakadr pqvpstalld ssdlpddaqs nrrmysrefl lecsqssvat
      661 qmppdfpsld pevanlmvkq ekaptqspkh vsrsgsimkn lpaqiatkas giveapaqtk
      721 qfkrsgsspe fsakadrpqv pstalldssd lpddaqsnrr mysrefllec sqssvatqmp
      781 pdfpsldpev anlmvkqeka ptqspkhvsr sgsimknlpa qiatkvsgiv eapaqtkqfk
      841 rsgsspefsa kadrpqvpst asldssdlpd daqsnrrmys refllecsqs svatqmppdf
      901 psldpevanl mvkqhitpfd sdgfrqrmmr qasvsdfvss spvsstpprl dlsevedsst
      961 t
//
LOCUS       XP_077502798             941 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113544 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502798
VERSION     XP_077502798.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646672.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..941
                     /product="uncharacterized protein LOC144113544 isoform X2"
                     /calculated_mol_wt=102762
     Region          219..290
                     /region_name="KH-I_Mextli_like"
                     /note="type I K homology (KH) RNA-binding domain found in
                     Drosophila melanogaster eukaryotic translation initiation
                     factor 4E-binding protein Mextli and similar proteins;
                     cd22454"
                     /db_xref="CDD:411882"
     Site            order(231,233..235,237..241,244..245,248..249,254..257)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411882"
     CDS             1..941
                     /gene="LOC144113544"
                     /coded_by="XM_077646672.1:166..2991"
                     /db_xref="GeneID:144113544"
ORIGIN      
        1 msakntkgvr klerprplrl nsdpekspss vlspsesvtm lidtnaqcav tdedvlkeie
       61 mlhisvnngn ydatilplia nvcdllklkg asmessfkdq ldcyfvtlrn asreerldvv
      121 grlrllevie lramgwkcnq nlteyykrtf aeleghefst ynsssnqmaf lpqlqvqpqq
      181 qqhtmtpiap atspplslqp tevlkssgkf gkpakipgrn flkdeivirn sdsgkvmgik
      241 grrvhmieel sdtvisfqrv npgvrdrlvq itgpseetit rakeliedti rrnaspvrem
      301 gdetsnynmg tkescqkpsl vhsysmgdas mgeytstitv grdvlrisga kavliktakl
      361 vldkyfsgqa efsdkvpdgl spsnqgsyms pepgvlsnnr gttdlcteta swqglavrrh
      421 sgppssqtss ssdeetyked geqelmekap tqspkhvsrs gsivknlpaq iatkasgive
      481 apaqtkqfkr sgsspefsak adrpqvpsta sldssdlpdd aqsnrrmysr efllecsqss
      541 vatqmppdfp sldpevanlm vkqekaptqs pkhvsrsgsi mknlpaqiat kasgiveapa
      601 qtkqfkrsgs spefsakadr pqvpstalld ssdlpddaqs nrrmysrefl lecsqssvat
      661 qmppdfpsld pevanlmvkq ekaptqspkh vsrsgsimkn lpaqiatkas giveapaqtk
      721 qfkrsgsspe fsakadrpqv pstalldssd lpddaqsnrr mysrefllec sqssvatqmp
      781 pdfpsldpev anlmvkqeka ptqspkhvsr sgsimknlpa qiatkvsgiv eapaqtkqfk
      841 rsgsspefsa kadrpqvpst asldssdlpd daqsnrrmys refllecsqs svatqmppdf
      901 psldpevanl mvkqvrhakt dgyakrkyst taaqvkgphq g
//
LOCUS       XP_077502799             937 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113544 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502799
VERSION     XP_077502799.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646673.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..937
                     /product="uncharacterized protein LOC144113544 isoform X3"
                     /calculated_mol_wt=102210
     Region          219..266
                     /region_name="KH-I"
                     /note="K homology (KH) RNA-binding domain, type I;
                     cl00098"
                     /db_xref="CDD:469614"
     CDS             1..937
                     /gene="LOC144113544"
                     /coded_by="XM_077646673.1:171..2984"
                     /db_xref="GeneID:144113544"
ORIGIN      
        1 msakntkgvr klerprplrl nsdpekspss vlspsesvtm lidtnaqcav tdedvlkeie
       61 mlhisvnngn ydatilplia nvcdllklkg asmessfkdq ldcyfvtlrn asreerldvv
      121 grlrllevie lramgwkcnq nlteyykrtf aeleghefst ynsssnqmaf lpqlqvqpqq
      181 qqhtmtpiap atspplslqp tevlkssgkf gkpakipgrn flkdeivirn sdsgkvnpgv
      241 rdrlvqitgp seetitrake liedtirrna spvremgdet snynmgtkes cqkpslvhsy
      301 smgdasmgey tstitvgrdv lrisgakavl iktaklvldk yfsgqaefsd kvpdglspsn
      361 qgsymspepg vlsnnrgttd lctetaswqg lavrrhsgpp ssqtssssde etykedgeqe
      421 lmekaptqsp khvsrsgsiv knlpaqiatk asgiveapaq tkqfkrsgss pefsakadrp
      481 qvpstaslds sdlpddaqsn rrmysrefll ecsqssvatq mppdfpsldp evanlmvkqe
      541 kaptqspkhv srsgsimknl paqiatkasg iveapaqtkq fkrsgsspef sakadrpqvp
      601 stalldssdl pddaqsnrrm ysrefllecs qssvatqmpp dfpsldpeva nlmvkqekap
      661 tqspkhvsrs gsimknlpaq iatkasgive apaqtkqfkr sgsspefsak adrpqvpsta
      721 lldssdlpdd aqsnrrmysr efllecsqss vatqmppdfp sldpevanlm vkqekaptqs
      781 pkhvsrsgsi mknlpaqiat kvsgiveapa qtkqfkrsgs spefsakadr pqvpstasld
      841 ssdlpddaqs nrrmysrefl lecsqssvat qmppdfpsld pevanlmvkq hitpfdsdgf
      901 rqrmmrqasv sdfvssspvs stpprldlse vedsstt
//
LOCUS       XP_077502800             917 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113544 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077502800
VERSION     XP_077502800.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646674.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..917
                     /product="uncharacterized protein LOC144113544 isoform X4"
                     /calculated_mol_wt=99978
     Region          219..266
                     /region_name="KH-I"
                     /note="K homology (KH) RNA-binding domain, type I;
                     cl00098"
                     /db_xref="CDD:469614"
     CDS             1..917
                     /gene="LOC144113544"
                     /coded_by="XM_077646674.1:171..2924"
                     /db_xref="GeneID:144113544"
ORIGIN      
        1 msakntkgvr klerprplrl nsdpekspss vlspsesvtm lidtnaqcav tdedvlkeie
       61 mlhisvnngn ydatilplia nvcdllklkg asmessfkdq ldcyfvtlrn asreerldvv
      121 grlrllevie lramgwkcnq nlteyykrtf aeleghefst ynsssnqmaf lpqlqvqpqq
      181 qqhtmtpiap atspplslqp tevlkssgkf gkpakipgrn flkdeivirn sdsgkvnpgv
      241 rdrlvqitgp seetitrake liedtirrna spvremgdet snynmgtkes cqkpslvhsy
      301 smgdasmgey tstitvgrdv lrisgakavl iktaklvldk yfsgqaefsd kvpdglspsn
      361 qgsymspepg vlsnnrgttd lctetaswqg lavrrhsgpp ssqtssssde etykedgeqe
      421 lmekaptqsp khvsrsgsiv knlpaqiatk asgiveapaq tkqfkrsgss pefsakadrp
      481 qvpstaslds sdlpddaqsn rrmysrefll ecsqssvatq mppdfpsldp evanlmvkqe
      541 kaptqspkhv srsgsimknl paqiatkasg iveapaqtkq fkrsgsspef sakadrpqvp
      601 stalldssdl pddaqsnrrm ysrefllecs qssvatqmpp dfpsldpeva nlmvkqekap
      661 tqspkhvsrs gsimknlpaq iatkasgive apaqtkqfkr sgsspefsak adrpqvpsta
      721 lldssdlpdd aqsnrrmysr efllecsqss vatqmppdfp sldpevanlm vkqekaptqs
      781 pkhvsrsgsi mknlpaqiat kvsgiveapa qtkqfkrsgs spefsakadr pqvpstasld
      841 ssdlpddaqs nrrmysrefl lecsqssvat qmppdfpsld pevanlmvkq vrhaktdgya
      901 krkysttaaq vkgphqg
//
LOCUS       XP_077502801             915 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113544 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077502801
VERSION     XP_077502801.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646675.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..915
                     /product="uncharacterized protein LOC144113544 isoform X5"
                     /calculated_mol_wt=99977
     Region          173..244
                     /region_name="KH-I_Mextli_like"
                     /note="type I K homology (KH) RNA-binding domain found in
                     Drosophila melanogaster eukaryotic translation initiation
                     factor 4E-binding protein Mextli and similar proteins;
                     cd22454"
                     /db_xref="CDD:411882"
     Site            order(185,187..189,191..195,198..199,202..203,208..211)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411882"
     CDS             1..915
                     /gene="LOC144113544"
                     /coded_by="XM_077646675.1:135..2882"
                     /db_xref="GeneID:144113544"
ORIGIN      
        1 mcavtdedvl keiemlhisv nngnydatil plianvcdll klkgasmess fkdqldcyfv
       61 tlrnasreer ldvvgrlrll evielramgw kcnqnlteyy krtfaelegh efstynsssn
      121 qmaflpqlqv qpqqqqhtmt piapatsppl slqptevlks sgkfgkpaki pgrnflkdei
      181 virnsdsgkv mgikgrrvhm ieelsdtvis fqrvnpgvrd rlvqitgpse etitrakeli
      241 edtirrnasp vremgdetsn ynmgtkescq kpslvhsysm gdasmgeyts titvgrdvlr
      301 isgakavlik taklvldkyf sgqaefsdkv pdglspsnqg symspepgvl snnrgttdlc
      361 tetaswqgla vrrhsgppss qtssssdeet ykedgeqelm ekaptqspkh vsrsgsivkn
      421 lpaqiatkas giveapaqtk qfkrsgsspe fsakadrpqv pstasldssd lpddaqsnrr
      481 mysrefllec sqssvatqmp pdfpsldpev anlmvkqeka ptqspkhvsr sgsimknlpa
      541 qiatkasgiv eapaqtkqfk rsgsspefsa kadrpqvpst alldssdlpd daqsnrrmys
      601 refllecsqs svatqmppdf psldpevanl mvkqekaptq spkhvsrsgs imknlpaqia
      661 tkasgiveap aqtkqfkrsg sspefsakad rpqvpstall dssdlpddaq snrrmysref
      721 llecsqssva tqmppdfpsl dpevanlmvk qekaptqspk hvsrsgsimk nlpaqiatkv
      781 sgiveapaqt kqfkrsgssp efsakadrpq vpstasldss dlpddaqsnr rmysreflle
      841 csqssvatqm ppdfpsldpe vanlmvkqhi tpfdsdgfrq rmmrqasvsd fvssspvsst
      901 pprldlseve dsstt
//
LOCUS       XP_077502802             844 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113544 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077502802
VERSION     XP_077502802.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646676.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..844
                     /product="uncharacterized protein LOC144113544 isoform X6"
                     /calculated_mol_wt=92335
     Region          219..290
                     /region_name="KH-I_Mextli_like"
                     /note="type I K homology (KH) RNA-binding domain found in
                     Drosophila melanogaster eukaryotic translation initiation
                     factor 4E-binding protein Mextli and similar proteins;
                     cd22454"
                     /db_xref="CDD:411882"
     Site            order(231,233..235,237..241,244..245,248..249,254..257)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411882"
     CDS             1..844
                     /gene="LOC144113544"
                     /coded_by="XM_077646676.1:166..2700"
                     /db_xref="GeneID:144113544"
ORIGIN      
        1 msakntkgvr klerprplrl nsdpekspss vlspsesvtm lidtnaqcav tdedvlkeie
       61 mlhisvnngn ydatilplia nvcdllklkg asmessfkdq ldcyfvtlrn asreerldvv
      121 grlrllevie lramgwkcnq nlteyykrtf aeleghefst ynsssnqmaf lpqlqvqpqq
      181 qqhtmtpiap atspplslqp tevlkssgkf gkpakipgrn flkdeivirn sdsgkvmgik
      241 grrvhmieel sdtvisfqrv npgvrdrlvq itgpseetit rakeliedti rrnaspvrem
      301 gdetsnynmg tkescqkpsl vhsysmgdas mgeytstitv grdvlrisga kavliktakl
      361 vldkyfsgqa efsdkvpdgl spsnqgsyms pepgvlsnnr gttdlcteta swqglavrrh
      421 sgppssqtss ssdeetyked geqelmekap tqspkhvsrs gsivknlpaq iatkasgive
      481 apaqtkqfkr sgsspefsak adrpqvpsta sldssdlpdd aqsnrrmysr efllecsqss
      541 vatqmppdfp sldpevanlm vkqekaptqs pkhvsrsgsi mknlpaqiat kasgiveapa
      601 qtkqfkrsgs spefsakadr pqvpstalld ssdlpddaqs nrrmysrefl lecsqssvat
      661 qmppdfpsld pevanlmvkq ekaptqspkh vsrsgsimkn lpaqiatkas giveapaqtk
      721 qfkrsgsspe fsakadrpqv pstalldssd lpddaqsnrr mysrefllec sqssvatqmp
      781 pdfpsldpev anlmvkqhit pfdsdgfrqr mmrqasvsdf vssspvsstp prldlseved
      841 sstt
//
LOCUS       XP_077502803             844 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113544 isoform X7 [Amblyomma
            americanum].
ACCESSION   XP_077502803
VERSION     XP_077502803.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646677.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..844
                     /product="uncharacterized protein LOC144113544 isoform X7"
                     /calculated_mol_wt=92395
     Region          219..290
                     /region_name="KH-I_Mextli_like"
                     /note="type I K homology (KH) RNA-binding domain found in
                     Drosophila melanogaster eukaryotic translation initiation
                     factor 4E-binding protein Mextli and similar proteins;
                     cd22454"
                     /db_xref="CDD:411882"
     Site            order(231,233..235,237..241,244..245,248..249,254..257)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411882"
     CDS             1..844
                     /gene="LOC144113544"
                     /coded_by="XM_077646677.1:166..2700"
                     /db_xref="GeneID:144113544"
ORIGIN      
        1 msakntkgvr klerprplrl nsdpekspss vlspsesvtm lidtnaqcav tdedvlkeie
       61 mlhisvnngn ydatilplia nvcdllklkg asmessfkdq ldcyfvtlrn asreerldvv
      121 grlrllevie lramgwkcnq nlteyykrtf aeleghefst ynsssnqmaf lpqlqvqpqq
      181 qqhtmtpiap atspplslqp tevlkssgkf gkpakipgrn flkdeivirn sdsgkvmgik
      241 grrvhmieel sdtvisfqrv npgvrdrlvq itgpseetit rakeliedti rrnaspvrem
      301 gdetsnynmg tkescqkpsl vhsysmgdas mgeytstitv grdvlrisga kavliktakl
      361 vldkyfsgqa efsdkvpdgl spsnqgsyms pepgvlsnnr gttdlcteta swqglavrrh
      421 sgppssqtss ssdeetyked geqelmekap tqspkhvsrs gsimknlpaq iatkasgive
      481 apaqtkqfkr sgsspefsak adrpqvpsta lldssdlpdd aqsnrrmysr efllecsqss
      541 vatqmppdfp sldpevanlm vkqekaptqs pkhvsrsgsi mknlpaqiat kasgiveapa
      601 qtkqfkrsgs spefsakadr pqvpstalld ssdlpddaqs nrrmysrefl lecsqssvat
      661 qmppdfpsld pevanlmvkq ekaptqspkh vsrsgsimkn lpaqiatkvs giveapaqtk
      721 qfkrsgsspe fsakadrpqv pstasldssd lpddaqsnrr mysrefllec sqssvatqmp
      781 pdfpsldpev anlmvkqhit pfdsdgfrqr mmrqasvsdf vssspvsstp prldlseved
      841 sstt
//
LOCUS       XP_077502804            4302 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113546 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502804
VERSION     XP_077502804.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646678.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..4302
                     /product="uncharacterized protein LOC144113546 isoform X1"
                     /calculated_mol_wt=485488
     CDS             1..4302
                     /gene="LOC144113546"
                     /coded_by="XM_077646678.1:41..12949"
                     /db_xref="GeneID:144113546"
ORIGIN      
        1 mavtalnqlt qnkfsmynwt ceikavtcme efisaqitnc stllanfvlp yakkgatwsq
       61 lktackghrd yqscvlhqgf kdcedgtnda klleaitngs fsayswtcet qgkvcreeyl
      121 encstiittv vspnikephe lyklrqackg vhgyqacvne mntacpvegi ytrsnmsevt
      181 hdvfnaynwt cnyegctnqt lkfalskces vldtsvkpya lnrsaddmld kackglhmye
      241 dcvaevhkgf cpnsslnsve lldqaarave mynwtcitgs dnctnanlek nihqctqtle
      301 advipyldrv ssvqvaeilc khvreyqici lqgskalcpa vdspdvsnln eltsgtytky
      361 ewictfspka cntsafepil aqcskaitdl vipnvemtkd sekireacng hkkfracime
      421 lgpgvcphtd frdeasavnl trglftqysw tcaeqgkics neeskaavdv csgiistkil
      481 qninvqdrlq lrnsceglre yvqcvnstlt akcptdlrlg rislsnvtke vetrygwtcs
      541 vkamnctpen akalvatcnh iledsvvpat rvsvfdlklp vtckevkryq dcivtegkki
      601 cphddmedvn vlsditrgnf skynwtcstd ltnctsanfi disnkcqkll elkvapnirl
      661 paskdvldva cvyvtkyqdc marksfeicp veltvtrewq qnatdglfsk yfwvcepqkv
      721 sctveklsev iescedqiys yvipysanyt dkkslkfacq gnkkyqdcii yhgnslcpdv
      781 efdinllnkl tknmftkynw tcmengtncs tqvmtsvlek cspiissdvi plvdesseen
      841 lsaackgvhr yqecaeqavr svcfqdsvla tfnfdnatag lyseynwtce gskpslevsp
      901 kcdartlgqr aseclgfikv kvrpfakdkt liaflktack nnnlfkqcin skipasclky
      961 dkllesdelk qfinvyedyr wtcklgitsg sacnakigkn lvecagiidv lvakdvsvfh
     1021 spsevaetcr hlksyeicmm qsikgvcgdg vvftqnsnvl kymieyynky swacvqapek
     1081 sctsealtak mkecrgfidf ivrpnaalsf smqdscqglq dyktcvftgi mescapnate
     1141 iannsvvdqy lhqvmekhas vcrqapepvg eckekavtek lrrcrgivsw lvlpnmdnpd
     1201 dddkqqracv avadyqacva ksvrqmcpnt nvysddtmdd ltgglytkys stcrhrrkmt
     1261 tssaapslts tvvhikkaph cseksatkal mecmgfmhvk vmpnaaarqn pemlkvacss
     1321 mkhyqqcvne kirmcanhse vteslliryy tdelyrkyew tcepatpkav cnpllvlghl
     1381 encsrqlnat vhglnpeevs awkteaacta vttyqecvss klnmkcahrv lpqtphvlhy
     1441 vrdlynaynw tcqagqvdkc dgnelmdals eclgflnvlv lpyitnsskn asletactgy
     1501 qefkycisss inakcprspy alrlprvqyf thdlvqnykw tcdavhqkkr qacrsetlla
     1561 qlekcfgehv rakvipnadt sagqdklqla cralntyqdc vneeiigsca gnqgqvllld
     1621 svknytysvy eqyqwtcdnh pdcnknslls elnsckgimt tkvipnavnm sdtsrlseac
     1681 rgtrlyqecv rekmsakcap espvfllpev ahfsfatfnk ynwtcfrkre grmsefkntt
     1741 elvkqlkecy gfvlfgvkrp lrssfkdkam reacmsardy qrcirakldq fskqqqslpd
     1801 sqdveyyatr lyskynwtcd slnvrlkshh cnvdrleagl kqcrsladfa iaavknktev
     1861 dissiceyyl dhqtcidnql qecrffpsvl klsrirrhtq llndsyrnyc sfllpgkpkc
     1921 ssaalsemye eclgfisygv vpnlgsdtae ldlpavqryl dnykgciqem tkiacgstdm
     1981 sqdlqvsltn pvqivkkysh fistnncses slgsalrecd giwdldvepl lkytdnsdli
     2041 qacssieifg ncvkqkvipl cekmeiasah qsvhaylsna ldvykticsr dlnavneamc
     2101 sktelvaafs ecggiieyrv vpfvddpydq vrlgvacsgv reykrcifsr lspvcedkiv
     2161 pmssdvtkyl rnnynlydwt cnttepqnkc dkeqlrkela kcigllnkef disvmglnte
     2221 hlctfledyk lclsmrtspe crkdddamrs pevlfftndv iskfmglcnm idvkecnrnt
     2281 vlesvykckg lvdyvlidsi qdsgdmeatk qacgglfnyr hcvhseifka cdasnpvlqe
     2341 ktvqrytvgv prnlswvcel ntkpedctvk nilpsitecq gfiagigdph hlrksdsfal
     2401 niacsfvqqy recidlnirs vckepkvlse iseisfytqd lyhkylwtcd rkktagelcn
     2461 vdelvshlkq cevflipgvv pyvdntfetl kysyvcgsls ayskcvqkak sticgnkeim
     2521 aspeikmlly nkineykwyc ssrksgsgdc neehledelk hclgfvktvv rpkllgsyna
     2581 tdmkmacsal qdyrycvvtk iskhcgydtt ivqrpairky lfdlydkhkw icqdyvktec
     2641 tarhvmahak scelhlnyts lsqltlrdtd sstmvcrglh qyqscfersy ldlcagdrsv
     2701 hlwntisgma akrakhaqea clkhkdpinl dscnpkvlae rsrrcqgivd tnlipnirkk
     2761 nnspklkeac saykqfqlcl rqnighcnis lapnkdvlek ykwvcsdaln ksltdtcnet
     2821 ellhemqgcl gimyflvipn sgqknnkqah akaceglkhy qncvlsktsr vcankilvrs
     2881 tkvrrltrhi mkkfswtcal ngqdqkckth sivdqlktcl gfidmavapn tfypadkakm
     2941 qmacsgledy qdcvdskvye mcpplhkylk rkdvrvftef lpskyswvch tsnshqctlg
     3001 nlmenlkicn glitsvlygk ldggklvgdn qamacsvmqs felcvtvqag ricnpddlvl
     3061 hhhdvraatt eildayswic ndtsavestf vdgavmwrks gceddllrdk lthcqelfnr
     3121 tvpaeftgek fqtgdveltc kavksyeeca rsaqlelcst tgenaivsey srelykeynw
     3181 tcnlasdalc desfllksip acievlksev ithvgtisna kaqklacegy kryqscvkyi
     3241 vdsscqnidi dntashvgim gfltrrlass fqwacngfhc stasvttsir ecvrtleels
     3301 nsfvrndrid pcekvtaykr cilkkmdlpc rlnhqflqsk elehytdvly safdgfctgv
     3361 qtecnkedml qslykceeii lkqvqphttd rgnpyesrla crglreykwc vsnvkttfhc
     3421 kgddefdaeg dvdklvvaie dkynwtceqd aktcvvpkfk ihlkpcfsil aadvlphild
     3481 ydnasgmdta crgvrhyqvc vskklyslck kekavyssem qyfkekvpkl lswtcswtst
     3541 kgrcdvhgml qkmavcasdl qqagvpsgpa nhsisekmag crafttyktc lsgtipemcs
     3601 qdaslmaska vhhftvdips qyarycdykd ggveiarqvs rcdeetlrir yiecsqiiss
     3661 evipftdeps dspllsdack amrkyqncia kiptqdcsng lkipfseesr yyvsvlkkky
     3721 ewvcngngdy mcnpeillly lskceatfmk srvhdyvdvd vldntdcrdl qnyqwclrns
     3781 ifesdceyyq niltspevly ftdkllrpyt ktchmnldcq igslyselse cipiikvkvl
     3841 pfvqqgwvqn pvtacsgtre yqkcfrhamq ktscdknigl fklaevqyyr sflfnkynwt
     3901 cfehaapvgs ceknqvqrdl qkcgssifqn taskkplscs slsmanflnc vdktnvklac
     3961 sadpekvssi aaqarevlld yraacgfert vvppgikflk kefercklvl kksdrllksa
     4021 qvretkvlda cralqlfslc teaaiilakn vdsphaaeir neseamvssy eikcaphecn
     4081 rnrvlddllr cftespvfsn cssslktstt tlaepcltkv fkghacsrkv dvvyvarevf
     4141 takveahkhd chmlksqyrq csqsefaifl qkcyesflre vtgkslatpk fcrmyhkygs
     4201 cvdeasaets cfnvtrlqap yevslrnfts kyaevctqyi rstgnngsss pghqplvrdi
     4261 edapaadkgf vprqisrivi ylclailmap ilfspfgfqh yl
//
LOCUS       XP_077502805            4301 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113546 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502805
VERSION     XP_077502805.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646679.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..4301
                     /product="uncharacterized protein LOC144113546 isoform X2"
                     /calculated_mol_wt=485417
     CDS             1..4301
                     /gene="LOC144113546"
                     /coded_by="XM_077646679.1:41..12946"
                     /db_xref="GeneID:144113546"
ORIGIN      
        1 mavtalnqlt qnkfsmynwt ceikavtcme efisaqitnc stllanfvlp yakkgatwsq
       61 lktackghrd yqscvlhqgf kdcedgtnda klleaitngs fsayswtcet qgkvcreeyl
      121 encstiittv vspnikephe lyklrqackg vhgyqacvne mntacpvegi ytrsnmsevt
      181 hdvfnaynwt cnyegctnqt lkfalskces vldtsvkpya lnrsaddmld kackglhmye
      241 dcvaevhkgf cpnsslnsve lldqaarave mynwtcitgs dnctnanlek nihqctqtle
      301 advipyldrv ssvqvaeilc khvreyqici lqgskalcpa vdspdvsnln eltsgtytky
      361 ewictfspka cntsafepil aqcskaitdl vipnvemtkd sekireacng hkkfracime
      421 lgpgvcphtd frdeasavnl trglftqysw tcaeqgkics neeskaavdv csgiistkil
      481 qninvqdrlq lrnsceglre yvqcvnstlt akcptdlrlg rislsnvtke vetrygwtcs
      541 vkamnctpen akalvatcnh iledsvvpat rvsvfdlklp vtckevkryq dcivtegkki
      601 cphddmedvn vlsditrgnf skynwtcstd ltnctsanfi disnkcqkll elkvapnirl
      661 paskdvldva cvyvtkyqdc marksfeicp veltvtrewq qnatdglfsk yfwvcepqkv
      721 sctveklsev iescedqiys yvipysanyt dkkslkfacq gnkkyqdcii yhgnslcpdv
      781 efdinllnkl tknmftkynw tcmengtncs tqvmtsvlek cspiissdvi plvdesseen
      841 lsaackgvhr yqecaeqavr svcfqdsvla tfnfdnatag lyseynwtce gskpslevsp
      901 kcdartlgqr aseclgfikv kvrpfakdkt liaflktack nnnlfkqcin skipasclky
      961 dkllesdelk qfinvyedyr wtcklgitsg sacnakigkn lvecagiidv lvakdvsvfh
     1021 spsevaetcr hlksyeicmm qsikgvcgdg vvftqnsnvl kymieyynky swacvqapek
     1081 sctsealtak mkecrgfidf ivrpnaalsf smqdscqglq dyktcvftgi mescapnate
     1141 iannsvvdqy lhqvmekhas vcrqapepvg eckekavtek lrrcrgivsw lvlpnmdnpd
     1201 dddkqqracv avadyqacva ksvrqmcpnt nvysddtmdd ltgglytkys stcrhrrkmt
     1261 tssaapslts tvvhikkaph cseksatkal mecmgfmhvk vmpnaaarqn pemlkvacss
     1321 mkhyqqcvne kirmcanhse vteslliryy tdelyrkyew tcepatpkav cnpllvlghl
     1381 encsrqlnat vhglnpeevs awkteaacta vttyqecvss klnmkcahrv lpqtphvlhy
     1441 vrdlynaynw tcqagqvdkc dgnelmdals eclgflnvlv lpyitnsskn asletactgy
     1501 qefkycisss inakcprspy alrlprvqyf thdlvqnykw tcdavhqkkr qacrsetlla
     1561 qlekcfgehv rakvipnadt sagqdklqla cralntyqdc vneeiigsca gnqgqvllld
     1621 svknytysvy eqyqwtcdnh pdcnknslls elnsckgimt tkvipnavnm sdtsrlseac
     1681 rgtrlyqecv rekmsakcap espvfllpev ahfsfatfnk ynwtcfrkre grmsefkntt
     1741 elvkqlkecy gfvlfgvkrp lrssfkdkam reacmsardy qrcirakldq fskqqqslpd
     1801 sqdveyyatr lyskynwtcd slnvrlkshh cnvdrleagl kqcrsladfa iaavknktev
     1861 dissiceyyl dhqtcidnql qecrffpsvl klsrirrhtq llndsyrnyc sfllpgkpkc
     1921 ssaalsemye eclgfisygv vpnlgsdtae ldlpavqryl dnykgciqem tkiacgstdm
     1981 sqdlqvsltn pvqivkkysh fistnncses slgsalrecd giwdldvepl lkytdnsdli
     2041 qacssieifg ncvkqkvipl cekmeiasah qsvhaylsna ldvykticsr dlnavneamc
     2101 sktelvaafs ecggiieyrv vpfvddpydq vrlgvacsgv reykrcifsr lspvcedkiv
     2161 pmssdvtkyl rnnynlydwt cnttepqnkc dkeqlrkela kcigllnkef disvmglnte
     2221 hlctfledyk lclsmrtspe crkdddamrs pevlfftndv iskfmglcnm idvkecnrnt
     2281 vlesvykckg lvdyvlidsi qdsgdmeatk qacgglfnyr hcvhseifka cdasnpvlqe
     2341 ktvqrytvgv prnlswvcel ntkpedctvk nilpsitecq gfiagigdph hlrksdsfal
     2401 niacsfvqqy recidlnirs vckepkvlse iseisfytqd lyhkylwtcd rkktgelcnv
     2461 delvshlkqc evflipgvvp yvdntfetlk ysyvcgslsa yskcvqkaks ticgnkeima
     2521 speikmllyn kineykwycs srksgsgdcn eehledelkh clgfvktvvr pkllgsynat
     2581 dmkmacsalq dyrycvvtki skhcgydtti vqrpairkyl fdlydkhkwi cqdyvktect
     2641 arhvmahaks celhlnytsl sqltlrdtds stmvcrglhq yqscfersyl dlcagdrsvh
     2701 lwntisgmaa krakhaqeac lkhkdpinld scnpkvlaer srrcqgivdt nlipnirkkn
     2761 nspklkeacs aykqfqlclr qnighcnisl apnkdvleky kwvcsdalnk sltdtcnete
     2821 llhemqgclg imyflvipns gqknnkqaha kaceglkhyq ncvlsktsrv cankilvrst
     2881 kvrrltrhim kkfswtcaln gqdqkckths ivdqlktclg fidmavapnt fypadkakmq
     2941 macsgledyq dcvdskvyem cpplhkylkr kdvrvftefl pskyswvcht snshqctlgn
     3001 lmenlkicng litsvlygkl dggklvgdnq amacsvmqsf elcvtvqagr icnpddlvlh
     3061 hhdvraatte ildayswicn dtsavestfv dgavmwrksg ceddllrdkl thcqelfnrt
     3121 vpaeftgekf qtgdveltck avksyeecar saqlelcstt genaivseys relykeynwt
     3181 cnlasdalcd esfllksipa cievlksevi thvgtisnak aqklacegyk ryqscvkyiv
     3241 dsscqnidid ntashvgimg fltrrlassf qwacngfhcs tasvttsire cvrtleelsn
     3301 sfvrndridp cekvtaykrc ilkkmdlpcr lnhqflqske lehytdvlys afdgfctgvq
     3361 tecnkedmlq slykceeiil kqvqphttdr gnpyesrlac rglreykwcv snvkttfhck
     3421 gddefdaegd vdklvvaied kynwtceqda ktcvvpkfki hlkpcfsila advlphildy
     3481 dnasgmdtac rgvrhyqvcv skklyslckk ekavyssemq yfkekvpkll swtcswtstk
     3541 grcdvhgmlq kmavcasdlq qagvpsgpan hsisekmagc rafttyktcl sgtipemcsq
     3601 daslmaskav hhftvdipsq yarycdykdg gveiarqvsr cdeetlriry iecsqiisse
     3661 vipftdepsd spllsdacka mrkyqnciak iptqdcsngl kipfseesry yvsvlkkkye
     3721 wvcngngdym cnpeilllyl skceatfmks rvhdyvdvdv ldntdcrdlq nyqwclrnsi
     3781 fesdceyyqn iltspevlyf tdkllrpytk tchmnldcqi gslyselsec ipiikvkvlp
     3841 fvqqgwvqnp vtacsgtrey qkcfrhamqk tscdkniglf klaevqyyrs flfnkynwtc
     3901 fehaapvgsc eknqvqrdlq kcgssifqnt askkplscss lsmanflncv dktnvklacs
     3961 adpekvssia aqarevlldy raacgfertv vppgikflkk efercklvlk ksdrllksaq
     4021 vretkvldac ralqlfslct eaaiilaknv dsphaaeirn eseamvssye ikcaphecnr
     4081 nrvlddllrc ftespvfsnc ssslktsttt laepcltkvf kghacsrkvd vvyvarevft
     4141 akveahkhdc hmlksqyrqc sqsefaiflq kcyesflrev tgkslatpkf crmyhkygsc
     4201 vdeasaetsc fnvtrlqapy evslrnftsk yaevctqyir stgnngsssp ghqplvrdie
     4261 dapaadkgfv prqisriviy lclailmapi lfspfgfqhy l
//
LOCUS       XP_077502806            1491 aa            linear   INV 25-NOV-2025
DEFINITION  puratrophin-1-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502806
VERSION     XP_077502806.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646680.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1491
                     /product="puratrophin-1-like isoform X1"
                     /calculated_mol_wt=167597
     Region          269..398
                     /region_name="CRAL_TRIO_2"
                     /note="Divergent CRAL/TRIO domain; pfam13716"
                     /db_xref="CDD:463965"
     Region          424..629
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cl02488"
                     /db_xref="CDD:413338"
     Site            523..528
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1010..1173
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          1173..1311
                     /region_name="PH_puratrophin-1"
                     /note="Puratrophin-1 pleckstrin homology (PH) domain;
                     cd13242"
                     /db_xref="CDD:270062"
     CDS             1..1491
                     /gene="LOC144113549"
                     /coded_by="XM_077646680.1:178..4653"
                     /db_xref="GeneID:144113549"
ORIGIN      
        1 mfkaspepas cddsgcpalq srkawplcfg rrvviqlcel dssvlrpgdf ylavgpedas
       61 rasgparlll kyrddlghlc srridahelg rllamdwtap gqetdeqlvt llkhllvsve
      121 rgleriewds llaanrswls ghrpdncsck ghasgpadps edqasglcpc skssgvdsas
      181 rrtsassgid pgpsssddss sregswagml lpdglsalfr ertslsreee hddgycdnsl
      241 vqptsmsqlg hrlllsrvac fpgcrdvngr giaivsssaf kdvpeltpsq laqmlmyfct
      301 ipkkeisvrg flllvdatda geqmwrmlde alclleanis naidtivvan srsplevkgl
      361 lpqtrtkvvh vtsqekllef vdkdnllpef ggtytydhqe wmsfrkflep fvsgcrlsgr
      421 qlvsvmqelr tsrvppssal alqlidaqkr hiqdtfrdeq lrhlqeegdt ilqelqayra
      481 rsphnldyrd aleksgllyn elkramaklt kladkrlqrl qecmlvrtfe eessqvlswv
      541 ckngaealsk hsfvadslsa iqdqeyefek fyflamrqie kgndlleeva massvtglak
      601 gtqelavslk hhldcftdkl edtrekledt srcyllldhs yewaldamkf vssmkmdnas
      661 tpesmtqlmr llqeyqeehp apseqnfsem ldlaskldnp klveqcktaq arcretaelm
      721 earhttlvra rqqlelegrk spslgtsfrs atmegcslfp qnswapgsts tpycsfvrrr
      781 siattqphly sapppmnsyt afpapggtsv vqdsfaldqy iqekeeqlls qglitddltr
      841 rgpvtsisrs flesrskegg prtaglsalr ekvrnslhkd ghqcrpqrkl mrrsytwqlc
      901 eealsrvsdr vraqnerlvs drtamadleg sdglassiss peseeltspd seqpkgegqs
      961 sdlvrgsrvp vpvnshlltr asalqladsa isdanfseeq lrnrktllli mremvqterd
     1021 yvksleyiie nyipelmred ipqalrgqrn vvfgniekiy efhslyflqq leqcescpfm
     1081 vgqcflqyep qfylyalynk nkpksdslms eygnaffkqk qlelgdkmdl asyllkpvqr
     1141 mgkyalllkq ilkecperep ehadlkaaee mvrfqlrhgn dllgmdalre cdvnvkeqgr
     1201 llrqdeflvw qgrsrkslrh vflfedlilf skvrrdpehk ghdiyqykhs ikttdiglte
     1261 qigesptkfe iwfrkrklnd vyllqapspe vkrawvhdis kllwkqalrn remrlaemss
     1321 mgignkpcld irpssdqind rsitfqqlsr taprlcsplg ilelgresga skrphsiisv
     1381 sssssssssq gslhcalnlg feagdsprpl qrslthqshc stesgfctda stagdcstda
     1441 slmtvrrhrk aersdsllsn dslathseeg shcgeevtss lhtqafslhd t
//
LOCUS       XP_077502807            1425 aa            linear   INV 25-NOV-2025
DEFINITION  puratrophin-1-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502807
VERSION     XP_077502807.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646681.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1425
                     /product="puratrophin-1-like isoform X2"
                     /calculated_mol_wt=160577
     Region          272..401
                     /region_name="CRAL_TRIO_2"
                     /note="Divergent CRAL/TRIO domain; pfam13716"
                     /db_xref="CDD:463965"
     Region          427..632
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cl02488"
                     /db_xref="CDD:413338"
     Site            526..531
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          944..1107
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          1107..1245
                     /region_name="PH_puratrophin-1"
                     /note="Puratrophin-1 pleckstrin homology (PH) domain;
                     cd13242"
                     /db_xref="CDD:270062"
     CDS             1..1425
                     /gene="LOC144113549"
                     /coded_by="XM_077646681.1:178..4455"
                     /db_xref="GeneID:144113549"
ORIGIN      
        1 mfkaspepas cddsgcpalq srkawplcfg rrvviqlcel dssvlrpgdf ylavgpedas
       61 rasgparlll kyrddlghlc srridahelg rllamdwtap gqetdeqlvt llkhllvsve
      121 rgleriewds llaanrswls ghrpdncsck ghasgpadps edqasglcpc skssgvdsas
      181 rrtsassgid pgpsssddss sregswagml lpgeldglsa lfrertslsr eeehddgycd
      241 nslvqptsms qlghrlllsr vacfpgcrdv ngrgiaivss safkdvpelt psqlaqmlmy
      301 fctipkkeis vrgflllvda tdageqmwrm ldealcllea nisnaidtiv vansrsplev
      361 kgllpqtrtk vvhvtsqekl lefvdkdnll pefggtytyd hqewmsfrkf lepfvsgcrl
      421 sgrqlvsvmq elrtsrvpps salalqlida qkrhiqdtfr deqlrhlqee gdtilqelqa
      481 yrarsphnld yrdaleksgl lynelkrama kltkladkrl qrlqecmlvr tfeeessqvl
      541 swvckngaea lskhsfvads lsaiqdqeye fekfyflamr qiekgndlle evamassvtg
      601 lakgtqelav slkhhldcft dkledtrekl edtsrcylll dhsyewalda mkfvssmkmd
      661 nastpesmtq lmrllqeyqe ehpapseqnf semldlaskl dnpklveqck taqarcreta
      721 elmearhttl vrarqqlele grkspslgts frsatmegcs lfpqnswapg ststpycsfv
      781 rrrsiattqp hlysapppmn sytafpapgg tsvvqdsfal dqyiqekeeq llsqglitdd
      841 ltrrgpvtsi srsflesrsk eggprtagls alrekvrnsl hkdghqcrpq rklmrrsytw
      901 qlceealsrv sdrvraqner lvsdrtamad legsdglart lllimremvq terdyvksle
      961 yiienyipel mredipqalr gqrnvvfgni ekiyefhsly flqqleqces cpfmvgqcfl
     1021 qyepqfylya lynknkpksd slmseygnaf fkqkqlelgd kmdlasyllk pvqrmgkyal
     1081 llkqilkecp erepehadlk aaeemvrfql rhgndllgmd alrecdvnvk eqgrllrqde
     1141 flvwqgrsrk slrhvflfed lilfskvrrd pehkghdiyq ykhsikttdi glteqigesp
     1201 tkfeiwfrkr klndvyllqa pspevkrawv hdiskllwkq alrnremrla emssmgignk
     1261 pcldirpssd qindrsitfq qlsrtaprlc splgilelgr esgaskrphs iisvssssss
     1321 sssqgslhca lnlgfeagds prplqrslth qshcstesgf ctdastagdc stdaslmtvr
     1381 rhrkaersds llsndslath seegshcgee vtsslhtqaf slhdt
//
LOCUS       XP_077502808            1400 aa            linear   INV 25-NOV-2025
DEFINITION  puratrophin-1-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077502808
VERSION     XP_077502808.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646682.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1400
                     /product="puratrophin-1-like isoform X3"
                     /calculated_mol_wt=157601
     Region          178..307
                     /region_name="CRAL_TRIO_2"
                     /note="Divergent CRAL/TRIO domain; pfam13716"
                     /db_xref="CDD:463965"
     Region          333..538
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cl02488"
                     /db_xref="CDD:413338"
     Site            432..437
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          919..1082
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          1082..1220
                     /region_name="PH_puratrophin-1"
                     /note="Puratrophin-1 pleckstrin homology (PH) domain;
                     cd13242"
                     /db_xref="CDD:270062"
     CDS             1..1400
                     /gene="LOC144113549"
                     /coded_by="XM_077646682.1:597..4799"
                     /db_xref="GeneID:144113549"
ORIGIN      
        1 mdwtapgqet deqlvtllkh llvsvergle riewdsllaa nrswlsghrp dncsckghas
       61 gpadpsedqa sglcpcskss gvdsasrrts assgidpgps ssddsssreg swagmllpge
      121 ldglsalfre rtslsreeeh ddgycdnslv qptsmsqlgh rlllsrvacf pgcrdvngrg
      181 iaivsssafk dvpeltpsql aqmlmyfcti pkkeisvrgf lllvdatdag eqmwrmldea
      241 lclleanisn aidtivvans rsplevkgll pqtrtkvvhv tsqekllefv dkdnllpefg
      301 gtytydhqew msfrkflepf vsgcrlsgrq lvsvmqelrt srvppssala lqlidaqkrh
      361 iqdtfrdeql rhlqeegdti lqelqayrar sphnldyrda leksgllyne lkramakltk
      421 ladkrlqrlq ecmlvrtfee essqvlswvc kngaealskh sfvadslsai qdqeyefekf
      481 yflamrqiek gndlleevam assvtglakg tqelavslkh hldcftdkle dtrekledts
      541 rcyllldhsy ewaldamkfv ssmkmdnast pesmtqlmrl lqeyqeehpa pseqnfseml
      601 dlaskldnpk lveqcktaqa rcretaelme arhttlvrar qqlelegrks pslgtsfrsa
      661 tmegcslfpq nswapgstst pycsfvrrrs iattqphlys apppmnsyta fpapggtsvv
      721 qdsfaldqyi qekeeqllsq glitddltrr gpvtsisrsf lesrskeggp rtaglsalre
      781 kvrnslhkdg hqcrpqrklm rrsytwqlce ealsrvsdrv raqnerlvsd rtamadlegs
      841 dglassissp eseeltspds eqpkgegqss dlvrgsrvpv pvnshlltra salqladsai
      901 sdanfseeql rnrktlllim remvqterdy vksleyiien yipelmredi pqalrgqrnv
      961 vfgniekiye fhslyflqql eqcescpfmv gqcflqyepq fylyalynkn kpksdslmse
     1021 ygnaffkqkq lelgdkmdla syllkpvqrm gkyalllkqi lkecperepe hadlkaaeem
     1081 vrfqlrhgnd llgmdalrec dvnvkeqgrl lrqdeflvwq grsrkslrhv flfedlilfs
     1141 kvrrdpehkg hdiyqykhsi kttdiglteq igesptkfei wfrkrklndv yllqapspev
     1201 krawvhdisk llwkqalrnr emrlaemssm gignkpcldi rpssdqindr sitfqqlsrt
     1261 aprlcsplgi lelgresgas krphsiisvs ssssssssqg slhcalnlgf eagdsprplq
     1321 rslthqshcs tesgfctdas tagdcstdas lmtvrrhrka ersdsllsnd slathseegs
     1381 hcgeevtssl htqafslhdt
//
LOCUS       XP_077502809            1397 aa            linear   INV 25-NOV-2025
DEFINITION  puratrophin-1-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077502809
VERSION     XP_077502809.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646683.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1397
                     /product="puratrophin-1-like isoform X4"
                     /calculated_mol_wt=157301
     Region          175..304
                     /region_name="CRAL_TRIO_2"
                     /note="Divergent CRAL/TRIO domain; pfam13716"
                     /db_xref="CDD:463965"
     Region          330..535
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cl02488"
                     /db_xref="CDD:413338"
     Site            429..434
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          916..1079
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          1079..1217
                     /region_name="PH_puratrophin-1"
                     /note="Puratrophin-1 pleckstrin homology (PH) domain;
                     cd13242"
                     /db_xref="CDD:270062"
     CDS             1..1397
                     /gene="LOC144113549"
                     /coded_by="XM_077646683.1:597..4790"
                     /db_xref="GeneID:144113549"
ORIGIN      
        1 mdwtapgqet deqlvtllkh llvsvergle riewdsllaa nrswlsghrp dncsckghas
       61 gpadpsedqa sglcpcskss gvdsasrrts assgidpgps ssddsssreg swagmllpdg
      121 lsalfrerts lsreeehddg ycdnslvqpt smsqlghrll lsrvacfpgc rdvngrgiai
      181 vsssafkdvp eltpsqlaqm lmyfctipkk eisvrgflll vdatdageqm wrmldealcl
      241 leanisnaid tivvansrsp levkgllpqt rtkvvhvtsq ekllefvdkd nllpefggty
      301 tydhqewmsf rkflepfvsg crlsgrqlvs vmqelrtsrv ppssalalql idaqkrhiqd
      361 tfrdeqlrhl qeegdtilqe lqayrarsph nldyrdalek sgllynelkr amakltklad
      421 krlqrlqecm lvrtfeeess qvlswvckng aealskhsfv adslsaiqdq eyefekfyfl
      481 amrqiekgnd lleevamass vtglakgtqe lavslkhhld cftdkledtr ekledtsrcy
      541 llldhsyewa ldamkfvssm kmdnastpes mtqlmrllqe yqeehpapse qnfsemldla
      601 skldnpklve qcktaqarcr etaelmearh ttlvrarqql elegrkspsl gtsfrsatme
      661 gcslfpqnsw apgststpyc sfvrrrsiat tqphlysapp pmnsytafpa pggtsvvqds
      721 faldqyiqek eeqllsqgli tddltrrgpv tsisrsfles rskeggprta glsalrekvr
      781 nslhkdghqc rpqrklmrrs ytwqlceeal srvsdrvraq nerlvsdrta madlegsdgl
      841 assisspese eltspdseqp kgegqssdlv rgsrvpvpvn shlltrasal qladsaisda
      901 nfseeqlrnr ktlllimrem vqterdyvks leyiienyip elmredipqa lrgqrnvvfg
      961 niekiyefhs lyflqqleqc escpfmvgqc flqyepqfyl yalynknkpk sdslmseygn
     1021 affkqkqlel gdkmdlasyl lkpvqrmgky alllkqilke cperepehad lkaaeemvrf
     1081 qlrhgndllg mdalrecdvn vkeqgrllrq deflvwqgrs rkslrhvflf edlilfskvr
     1141 rdpehkghdi yqykhsiktt diglteqige sptkfeiwfr krklndvyll qapspevkra
     1201 wvhdiskllw kqalrnremr laemssmgig nkpcldirps sdqindrsit fqqlsrtapr
     1261 lcsplgilel gresgaskrp hsiisvssss sssssqgslh calnlgfeag dsprplqrsl
     1321 thqshcstes gfctdastag dcstdaslmt vrrhrkaers dsllsndsla thseegshcg
     1381 eevtsslhtq afslhdt
//
LOCUS       XP_077502810            1494 aa            linear   INV 25-NOV-2025
DEFINITION  puratrophin-1-like isoform X5 [Amblyomma americanum].
ACCESSION   XP_077502810
VERSION     XP_077502810.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646684.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1494
                     /product="puratrophin-1-like isoform X5"
                     /calculated_mol_wt=167897
     Region          272..401
                     /region_name="CRAL_TRIO_2"
                     /note="Divergent CRAL/TRIO domain; pfam13716"
                     /db_xref="CDD:463965"
     Region          427..632
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cl02488"
                     /db_xref="CDD:413338"
     Site            526..531
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1013..1176
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          1176..1314
                     /region_name="PH_puratrophin-1"
                     /note="Puratrophin-1 pleckstrin homology (PH) domain;
                     cd13242"
                     /db_xref="CDD:270062"
     CDS             1..1494
                     /gene="LOC144113549"
                     /coded_by="XM_077646684.1:235..4719"
                     /db_xref="GeneID:144113549"
ORIGIN      
        1 mfkaspepas cddsgcpalq srkawplcfg rrvviqlcel dssvlrpgdf ylavgpedas
       61 rasgparlll kyrddlghlc srridahelg rllamdwtap gqetdeqlvt llkhllvsve
      121 rgleriewds llaanrswls ghrpdncsck ghasgpadps edqasglcpc skssgvdsas
      181 rrtsassgid pgpsssddss sregswagml lpgeldglsa lfrertslsr eeehddgycd
      241 nslvqptsms qlghrlllsr vacfpgcrdv ngrgiaivss safkdvpelt psqlaqmlmy
      301 fctipkkeis vrgflllvda tdageqmwrm ldealcllea nisnaidtiv vansrsplev
      361 kgllpqtrtk vvhvtsqekl lefvdkdnll pefggtytyd hqewmsfrkf lepfvsgcrl
      421 sgrqlvsvmq elrtsrvpps salalqlida qkrhiqdtfr deqlrhlqee gdtilqelqa
      481 yrarsphnld yrdaleksgl lynelkrama kltkladkrl qrlqecmlvr tfeeessqvl
      541 swvckngaea lskhsfvads lsaiqdqeye fekfyflamr qiekgndlle evamassvtg
      601 lakgtqelav slkhhldcft dkledtrekl edtsrcylll dhsyewalda mkfvssmkmd
      661 nastpesmtq lmrllqeyqe ehpapseqnf semldlaskl dnpklveqck taqarcreta
      721 elmearhttl vrarqqlele grkspslgts frsatmegcs lfpqnswapg ststpycsfv
      781 rrrsiattqp hlysapppmn sytafpapgg tsvvqdsfal dqyiqekeeq llsqglitdd
      841 ltrrgpvtsi srsflesrsk eggprtagls alrekvrnsl hkdghqcrpq rklmrrsytw
      901 qlceealsrv sdrvraqner lvsdrtamad legsdglass isspeseelt spdseqpkge
      961 gqssdlvrgs rvpvpvnshl ltrasalqla dsaisdanfs eeqlrnrktl llimremvqt
     1021 erdyvksley iienyipelm redipqalrg qrnvvfgnie kiyefhslyf lqqleqcesc
     1081 pfmvgqcflq yepqfylyal ynknkpksds lmseygnaff kqkqlelgdk mdlasyllkp
     1141 vqrmgkyall lkqilkecpe repehadlka aeemvrfqlr hgndllgmda lrecdvnvke
     1201 qgrllrqdef lvwqgrsrks lrhvflfedl ilfskvrrdp ehkghdiyqy khsikttdig
     1261 lteqigespt kfeiwfrkrk lndvyllqap spevkrawvh diskllwkqa lrnremrlae
     1321 mssmgignkp cldirpssdq indrsitfqq lsrtaprlcs plgilelgre sgaskrphsi
     1381 isvsssssss ssqgslhcal nlgfeagdsp rplqrslthq shcstesgfc tdastagdcs
     1441 tdaslmtvrr hrkaersdsl lsndslaths eegshcgeev tsslhtqafs lhdt
//
LOCUS       XP_077502811             296 aa            linear   INV 25-NOV-2025
DEFINITION  purine nucleoside phosphorylase-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502811
VERSION     XP_077502811.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646685.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..296
                     /product="purine nucleoside phosphorylase-like isoform X1"
                     /calculated_mol_wt=32618
     Region          19..291
                     /region_name="PNP-EcPNPII_like"
                     /note="purine nucleoside phosphorylases similar to human
                     PNP and Escherichia coli PNP-II (XapA); cd09009"
                     /db_xref="CDD:350160"
     Site            order(43..44,95,97,99,125..129,207,212..213,218,229..232,
                     254..257,267,269,272)
                     /site_type="active"
                     /db_xref="CDD:350160"
     Site            order(43..44,75,95,97,126..127,232)
                     /site_type="other"
                     /note="phosphate binding site [ion binding]"
                     /db_xref="CDD:350160"
     Site            order(44,97,99,127..129,212..213,229..231,254..255,269,
                     272)
                     /site_type="other"
                     /note="purine nucleoside binding site [chemical binding]"
                     /db_xref="CDD:350160"
     Site            order(98..102,144..150,155..156,158..159,170..176,179,182,
                     186,203,206..217,219..220,222..224,231,238,258,265..267,
                     269)
                     /site_type="other"
                     /note="homotrimer interface [polypeptide binding]"
                     /db_xref="CDD:350160"
     CDS             1..296
                     /gene="LOC144113552"
                     /coded_by="XM_077646685.1:250..1140"
                     /db_xref="GeneID:144113552"
ORIGIN      
        1 makvpgqsse nsiegysyeh iesianflls ktehrptvgi icgsglgsla dliegskqfq
       61 ykeipdfpvs tvpghsgklv fgklegkivi cmqgrfhpye gyplwkcamp vrlmkllgvk
      121 tlivtnaagg vnpnfkagdi miirdhinfp glggdnplkg rnderwgprf pamsnaydrk
      181 lrelakkian eelglwpylr egvycmlggp nfesvaeinm lrvlgadacg mstaheviva
      241 rhcglrvfgl slisnecisn ydtqqvanhe evletgqkrk kdleklvrtl ikrmda
//
LOCUS       XP_077502812             288 aa            linear   INV 25-NOV-2025
DEFINITION  purine nucleoside phosphorylase-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502812
VERSION     XP_077502812.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646686.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..288
                     /product="purine nucleoside phosphorylase-like isoform X2"
                     /calculated_mol_wt=31908
     Region          11..283
                     /region_name="PNP-EcPNPII_like"
                     /note="purine nucleoside phosphorylases similar to human
                     PNP and Escherichia coli PNP-II (XapA); cd09009"
                     /db_xref="CDD:350160"
     Site            order(35..36,87,89,91,117..121,199,204..205,210,221..224,
                     246..249,259,261,264)
                     /site_type="active"
                     /db_xref="CDD:350160"
     Site            order(35..36,67,87,89,118..119,224)
                     /site_type="other"
                     /note="phosphate binding site [ion binding]"
                     /db_xref="CDD:350160"
     Site            order(36,89,91,119..121,204..205,221..223,246..247,261,
                     264)
                     /site_type="other"
                     /note="purine nucleoside binding site [chemical binding]"
                     /db_xref="CDD:350160"
     Site            order(90..94,136..142,147..148,150..151,162..168,171,174,
                     178,195,198..209,211..212,214..216,223,230,250,257..259,
                     261)
                     /site_type="other"
                     /note="homotrimer interface [polypeptide binding]"
                     /db_xref="CDD:350160"
     CDS             1..288
                     /gene="LOC144113552"
                     /coded_by="XM_077646686.1:114..980"
                     /db_xref="GeneID:144113552"
ORIGIN      
        1 msskdeqysy ehiesianfl lsktehrptv giicgsglgs ladliegskq fqykeipdfp
       61 vstvpghsgk lvfgklegki vicmqgrfhp yegyplwkca mpvrlmkllg vktlivtnaa
      121 ggvnpnfkag dimiirdhin fpglggdnpl kgrnderwgp rfpamsnayd rklrelakki
      181 aneelglwpy lregvycmlg gpnfesvaei nmlrvlgada cgmstahevi varhcglrvf
      241 glslisneci snydtqqvan heevletgqk rkkdleklvr tlikrmda
//
LOCUS       XP_077502813             391 aa            linear   INV 25-NOV-2025
DEFINITION  tRNA methyltransferase 10 homolog C-like [Amblyomma americanum].
ACCESSION   XP_077502813
VERSION     XP_077502813.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646687.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..391
                     /product="tRNA methyltransferase 10 homolog C-like"
                     /calculated_mol_wt=45264
     Region          207..382
                     /region_name="Trm10_MRRP1"
                     /note="Mitochondrial ribonuclease P protein 1; cd18102"
                     /db_xref="CDD:349975"
     Site            order(295..297,314..315,317,319,340..341,356..357,359,362)
                     /site_type="other"
                     /note="SAM binding site [chemical binding]"
                     /db_xref="CDD:349975"
     CDS             1..391
                     /gene="LOC144113553"
                     /coded_by="XM_077646687.1:146..1321"
                     /db_xref="GeneID:144113553"
ORIGIN      
        1 malrlwllql tahhsadris nrcwklmaaa tevpfstcrr ycnvvpahkl teeherydea
       61 netahpyrpy cvadfpglav dkitkerirl llrhhklrlr sgllaplels sqdvldllak
      121 dsfnkrcrff kylfhqeqkr lrqqrretdg lvtteapkst lgrllphsad rldeyrlfrn
      181 cflirirect inrfyesrqi pavlfgqsiv fdlsydevmt presnkaard llsvfgrnrc
      241 srnpfhlyfc naspngltar ilqsalfeps eipllseltp ssyldifpke klvyltpdse
      301 ntldvfdyda vyvvgalvdk eakkgatlak atseglktar fpqtfswywq kevsqplhlt
      361 dvfkillalk qtndwnsalr yvnrkvtvqq d
//
LOCUS       XP_077502814            1034 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: L-fucose kinase-like [Amblyomma americanum].
ACCESSION   XP_077502814
VERSION     XP_077502814.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646688.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1034
                     /product="LOW QUALITY PROTEIN: L-fucose kinase-like"
                     /calculated_mol_wt=112969
     Region          40..987
                     /region_name="fkp"
                     /note="bifunctional
                     fucokinase/L-fucose-1-P-guanylyltransferase; Provisional;
                     PRK13412"
                     /db_xref="CDD:237379"
     CDS             1..1034
                     /gene="LOC144113554"
                     /coded_by="XM_077646688.1:140..3244"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 1 base in 1 codon"
                     /db_xref="GeneID:144113554"
ORIGIN      
        1 mcnfdcivvt csslkwatai eaelrvlqar niisesvkil tvedpasnig sgaatlnall
       61 vvteflsahn gftvmspdvl dksrililhh sreyalepcg kafmsipeih aastdqitgr
      121 ssnlenllni vsqmlengpp gvwvcstdmi ltctdvavnw hsvkdcllic sasdpdyatn
      181 hgavrvnsdg vvsnilycga lealhnytrp dgtvlvvsgl vffsaelaes llalhsmspl
      241 dcctymgvds gaepmqmslf fdllvamaad vsqdsfvsgq cgrwydkkfc lektalaqra
      301 rnliwnelsp ysvnaytlgg kpshrylsnd ttgrqhsell inragsemlh haaldvsikr
      361 vgkcsilmns fircenccig snsllkdchl easevvigen cyinglavnv kdealvipan
      421 tcvlqyeihl enfntapvvi afgmddhlgl gqqqdclmta klftcgkesf rdalrlvgtt
      481 hcqenvdewk kfsrlsladv lvhqslkdqf hkcwniffrv asdiiektll eksdvlitpy
      541 mnaacaygre adlmavldsv aegkmasedl aiasrafsci adflgamagn agglrsgpgg
      601 nktwmcafql lmegqvqkga lalkqerlkw mdrpdrmmra arhyegalqv lirkavhtae
      661 kyiitapasq lppfgvwava ecparmdlfg gwtdtppicy elggsvinia vlvdgqkpig
      721 akarrlhepq ivitllhdlv pekieitsie dlldysqpga rgallkaclv gsgvveidsk
      781 nklseqllar hsggielqsw sklpqgsglg tssilaaaiv aalwtavgqt fdkqavihcv
      841 lhveqllttg ggwqdqvggv tgglvhgssq phlplhvdve ilplslefch rlsnhfllly
      901 tgkvrlaknl lqtvirnwyt retkvvscfx ellhlcktsv kesflqgdle aigkwldhyw
      961 qlkkilavgc epsfvarlmg llkpyvhgql llgaggggfl vpsprsrarq asfrgfwtdh
     1021 reclklpytk wkli
//
LOCUS       XP_077502815             384 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113555 [Amblyomma americanum].
ACCESSION   XP_077502815
VERSION     XP_077502815.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646689.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..384
                     /product="uncharacterized protein LOC144113555"
                     /calculated_mol_wt=40267
     CDS             1..384
                     /gene="LOC144113555"
                     /coded_by="XM_077646689.1:174..1328"
                     /db_xref="GeneID:144113555"
ORIGIN      
        1 mrfnvhfkil laglvigvgs stellpagtg eadssnnaqr tevvagtpav sseplvanpv
       61 ealekleapg gavidaghsd sargfapvpi gmpthealvl ipspmfepfa lpfhmpvpyd
      121 pvsrfspgvp yvihrhpalg agaggyplei yhpavpflpe lsylpplppp ppgypplppl
      181 pgrpggppsp pqlpkpqplp plpkpqvppp rrpqlppklp mpprqppkip lpkpsppvrp
      241 apprrppapp rkqprppisk papqplpvpk plpvprppsv prppvlpkpe rkpdpetgpv
      301 plpedgddte iadpqsdppv lseenapgrr lfpsysqpda iqprviadkv gggsdttgdt
      361 vgpdgavdvk sqqgsfydgq avev
//
LOCUS       XP_077502816             223 aa            linear   INV 25-NOV-2025
DEFINITION  kelch-like protein 17 [Amblyomma americanum].
ACCESSION   XP_077502816
VERSION     XP_077502816.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646690.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..223
                     /product="kelch-like protein 17"
                     /calculated_mol_wt=25462
     Region          26..163
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     CDS             1..223
                     /gene="LOC144113556"
                     /coded_by="XM_077646690.1:7680..8351"
                     /db_xref="GeneID:144113556"
ORIGIN      
        1 mpdfgawcgr pqdntrtgwp gqplrlrvrl fcpefladah amvtghksdy vvilgnwyrs
       61 addlvlhyel rlqtlpegwe rlrvssqaql ksliasapda gidnlfiape alrpfvsscp
      121 qllqrwrlql mrrefllfkv geepllrtse egkellieam ryhlmpewra amagprtstk
      181 qlaprcrhpc ttfwslmihc lthgvdqtsl fadiakhipg lef
//
LOCUS       XP_077502817             223 aa            linear   INV 25-NOV-2025
DEFINITION  kelch-like protein 17 [Amblyomma americanum].
ACCESSION   XP_077502817
VERSION     XP_077502817.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646691.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..223
                     /product="kelch-like protein 17"
                     /calculated_mol_wt=25462
     Region          26..163
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     CDS             1..223
                     /gene="LOC144113556"
                     /coded_by="XM_077646691.1:7680..8351"
                     /db_xref="GeneID:144113556"
ORIGIN      
        1 mpdfgawcgr pqdntrtgwp gqplrlrvrl fcpefladah amvtghksdy vvilgnwyrs
       61 addlvlhyel rlqtlpegwe rlrvssqaql ksliasapda gidnlfiape alrpfvsscp
      121 qllqrwrlql mrrefllfkv geepllrtse egkellieam ryhlmpewra amagprtstk
      181 qlaprcrhpc ttfwslmihc lthgvdqtsl fadiakhipg lef
//
LOCUS       XP_077502818             223 aa            linear   INV 25-NOV-2025
DEFINITION  kelch-like protein 17 [Amblyomma americanum].
ACCESSION   XP_077502818
VERSION     XP_077502818.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646692.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..223
                     /product="kelch-like protein 17"
                     /calculated_mol_wt=25462
     Region          26..163
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     CDS             1..223
                     /gene="LOC144113556"
                     /coded_by="XM_077646692.1:7680..8351"
                     /db_xref="GeneID:144113556"
ORIGIN      
        1 mpdfgawcgr pqdntrtgwp gqplrlrvrl fcpefladah amvtghksdy vvilgnwyrs
       61 addlvlhyel rlqtlpegwe rlrvssqaql ksliasapda gidnlfiape alrpfvsscp
      121 qllqrwrlql mrrefllfkv geepllrtse egkellieam ryhlmpewra amagprtstk
      181 qlaprcrhpc ttfwslmihc lthgvdqtsl fadiakhipg lef
//
LOCUS       XP_077502819             428 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113558 [Amblyomma americanum].
ACCESSION   XP_077502819
VERSION     XP_077502819.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646693.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..428
                     /product="uncharacterized protein LOC144113558"
                     /calculated_mol_wt=48187
     CDS             1..428
                     /gene="LOC144113558"
                     /coded_by="XM_077646693.1:127..1413"
                     /db_xref="GeneID:144113558"
ORIGIN      
        1 msmlernwdd ftknrhqffa tfvtsddiam ldvkdetcvp litdnygdwt ddiavtlgsd
       61 vlrrcigypv ervrqrlykt wlarfaaqew pdtfdqdlle cvvatpspsd ptkplfgdlf
      121 rlaavsprpe rtfldtstls plfkrpavld adfktakglv ayldgllyvd dqkalheagl
      181 iigflsllls svtavsvnrl mgifrdleki glatvlptsf sgefpcptsa nlenlrnrcg
      241 wgqsalrpyi palavaqytl ymraideapr edvlflersf mvrtkysgld cvdllemfar
      301 ktrlpmdyln rmlggkasaw trklmefyls nvaspgnmqk sapwcrvvdp tffrglevgd
      361 gpsyalkims fltrsprgll ykkwsrlahe tmpesevena rvwadnfral lakgqlrpve
      421 asmasatn
//
LOCUS       XP_077502820             194 aa            linear   INV 25-NOV-2025
DEFINITION  natterin-4-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502820
VERSION     XP_077502820.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646694.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..194
                     /product="natterin-4-like isoform X1"
                     /calculated_mol_wt=20855
     Region          69..181
                     /region_name="DUF3421"
                     /note="Protein of unknown function (DUF3421); pfam11901"
                     /db_xref="CDD:463390"
     CDS             1..194
                     /gene="LOC144113559"
                     /coded_by="XM_077646694.1:32..616"
                     /db_xref="GeneID:144113559"
ORIGIN      
        1 mqtlallhrl scsiygphlt rerdpasdqq lalctkmaaf gafhhqpvcq wvqchgsrlp
       61 snavpggeds gesifigram hggdlvpgkv vpthnccyvs ydgtehshqe yqalvtngtp
      121 mtwvpaacgs vptgaiqggs cangeplyig rtfhdgtlai gkvhpshgcl yipyggqehr
      181 yteyevfvck tinf
//
LOCUS       XP_077502821             115 aa            linear   INV 25-NOV-2025
DEFINITION  natterin-3-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502821
VERSION     XP_077502821.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646695.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..115
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..115
                     /product="natterin-3-like isoform X2"
                     /calculated_mol_wt=12270
     Region          1..102
                     /region_name="DUF3421"
                     /note="Protein of unknown function (DUF3421); pfam11901"
                     /db_xref="CDD:463390"
     CDS             1..115
                     /gene="LOC144113559"
                     /coded_by="XM_077646695.1:511..858"
                     /db_xref="GeneID:144113559"
ORIGIN      
        1 mhggdlvpgk vvpthnccyv sydgtehshq eyqalvtngt pmtwvpaacg svptgaiqgg
       61 scangeplyi grtfhdgtla igkvhpshgc lyipyggqeh ryteyevfvc ktinf
//
LOCUS       XP_077502822             135 aa            linear   INV 25-NOV-2025
DEFINITION  protein regulator of cytokinesis 1-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502822
VERSION     XP_077502822.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646696.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..135
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..135
                     /product="protein regulator of cytokinesis 1-like isoform
                     X1"
                     /calculated_mol_wt=16195
     Region          <3..103
                     /region_name="MAP65_ASE1"
                     /note="Microtubule associated protein (MAP65/ASE1 family);
                     pfam03999"
                     /db_xref="CDD:427641"
     CDS             1..135
                     /gene="LOC144113560"
                     /coded_by="XM_077646696.1:1535..1942"
                     /db_xref="GeneID:144113560"
ORIGIN      
        1 mqverrealc srflefekra adqsrlnhrg gcllemrark rletelprle eeikmyisrh
       61 tgpeeqflse spidflehls sphdaynlek drerqertkg lknfqeelrr fkefhaknkp
      121 ilmkvewreg svlrv
//
LOCUS       XP_077502823             135 aa            linear   INV 25-NOV-2025
DEFINITION  protein regulator of cytokinesis 1-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502823
VERSION     XP_077502823.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646697.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..135
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..135
                     /product="protein regulator of cytokinesis 1-like isoform
                     X1"
                     /calculated_mol_wt=16195
     Region          <3..103
                     /region_name="MAP65_ASE1"
                     /note="Microtubule associated protein (MAP65/ASE1 family);
                     pfam03999"
                     /db_xref="CDD:427641"
     CDS             1..135
                     /gene="LOC144113560"
                     /coded_by="XM_077646697.1:669..1076"
                     /db_xref="GeneID:144113560"
ORIGIN      
        1 mqverrealc srflefekra adqsrlnhrg gcllemrark rletelprle eeikmyisrh
       61 tgpeeqflse spidflehls sphdaynlek drerqertkg lknfqeelrr fkefhaknkp
      121 ilmkvewreg svlrv
//
LOCUS       XP_077502824             121 aa            linear   INV 25-NOV-2025
DEFINITION  protein regulator of cytokinesis 1-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502824
VERSION     XP_077502824.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646698.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..121
                     /product="protein regulator of cytokinesis 1-like isoform
                     X2"
                     /calculated_mol_wt=14314
     Region          <3..120
                     /region_name="MAP65_ASE1"
                     /note="Microtubule associated protein (MAP65/ASE1 family);
                     pfam03999"
                     /db_xref="CDD:427641"
     CDS             1..121
                     /gene="LOC144113560"
                     /coded_by="XM_077646698.1:805..1170"
                     /db_xref="GeneID:144113560"
ORIGIN      
        1 mqverrealw arslefemra adqsrlnhrd gcllemrark rletelprle eeiktyinrh
       61 tgleeqflse saidflehlc sphdaynlek drerqertrg lknrrknseg srsstpktsp
      121 f
//
LOCUS       XP_077502825             121 aa            linear   INV 25-NOV-2025
DEFINITION  protein regulator of cytokinesis 1-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502825
VERSION     XP_077502825.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646699.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..121
                     /product="protein regulator of cytokinesis 1-like isoform
                     X3"
                     /calculated_mol_wt=14314
     Region          <3..120
                     /region_name="MAP65_ASE1"
                     /note="Microtubule associated protein (MAP65/ASE1 family);
                     pfam03999"
                     /db_xref="CDD:427641"
     CDS             1..121
                     /gene="LOC144113560"
                     /coded_by="XM_077646699.1:805..1170"
                     /db_xref="GeneID:144113560"
ORIGIN      
        1 mqverrealw arslefemra adqsrlnhrd gcllemrark rletelprle eeiktyinrh
       61 tgleeqflse saidflehlc sphdaynlek drerqertrg lknrrknseg srsstpktsp
      121 f
//
LOCUS       XP_077502826             150 aa            linear   INV 25-NOV-2025
DEFINITION  protein regulator of cytokinesis 1-like [Amblyomma americanum].
ACCESSION   XP_077502826
VERSION     XP_077502826.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646700.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..150
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..150
                     /product="protein regulator of cytokinesis 1-like"
                     /calculated_mol_wt=17665
     Region          <8..149
                     /region_name="MAP65_ASE1"
                     /note="Microtubule associated protein (MAP65/ASE1 family);
                     pfam03999"
                     /db_xref="CDD:427641"
     CDS             1..150
                     /gene="LOC144113561"
                     /coded_by="XM_077646700.1:926..1378"
                     /db_xref="GeneID:144113561"
ORIGIN      
        1 magedqrlee peeelgsnke fhaknkpilm qverrealwa rflefekraa dqsrlnhrgg
       61 cllemrvrkr letelprlee eiktyisrht greeqflses aidflehlcs phdaynlekd
      121 rerqertrgl knrrknsegs rsstpntspf
//
LOCUS       XP_077502827             121 aa            linear   INV 25-NOV-2025
DEFINITION  protein regulator of cytokinesis 1-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502827
VERSION     XP_077502827.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646701.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..121
                     /product="protein regulator of cytokinesis 1-like isoform
                     X1"
                     /calculated_mol_wt=14314
     Region          <3..120
                     /region_name="MAP65_ASE1"
                     /note="Microtubule associated protein (MAP65/ASE1 family);
                     pfam03999"
                     /db_xref="CDD:427641"
     CDS             1..121
                     /gene="LOC144113562"
                     /coded_by="XM_077646701.1:1032..1397"
                     /db_xref="GeneID:144113562"
ORIGIN      
        1 mqverrealw arslefemra adqsrlnhrd gcllemrark rletelprle eeiktyinrh
       61 tgleeqflse saidflehlc sphdaynlek drerqertrg lknrrknseg srsstpktsp
      121 f
//
LOCUS       XP_077502828             121 aa            linear   INV 25-NOV-2025
DEFINITION  protein regulator of cytokinesis 1-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502828
VERSION     XP_077502828.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646702.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..121
                     /product="protein regulator of cytokinesis 1-like isoform
                     X1"
                     /calculated_mol_wt=14314
     Region          <3..120
                     /region_name="MAP65_ASE1"
                     /note="Microtubule associated protein (MAP65/ASE1 family);
                     pfam03999"
                     /db_xref="CDD:427641"
     CDS             1..121
                     /gene="LOC144113562"
                     /coded_by="XM_077646702.1:1032..1397"
                     /db_xref="GeneID:144113562"
ORIGIN      
        1 mqverrealw arslefemra adqsrlnhrd gcllemrark rletelprle eeiktyinrh
       61 tgleeqflse saidflehlc sphdaynlek drerqertrg lknrrknseg srsstpktsp
      121 f
//
LOCUS       XP_077502829             101 aa            linear   INV 25-NOV-2025
DEFINITION  protein regulator of cytokinesis 1-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502829
VERSION     XP_077502829.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646703.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..101
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..101
                     /product="protein regulator of cytokinesis 1-like isoform
                     X2"
                     /calculated_mol_wt=12160
     Region          <3..95
                     /region_name="MAP65_ASE1"
                     /note="Microtubule associated protein (MAP65/ASE1 family);
                     pfam03999"
                     /db_xref="CDD:427641"
     CDS             1..101
                     /gene="LOC144113562"
                     /coded_by="XM_077646703.1:1032..1337"
                     /db_xref="GeneID:144113562"
ORIGIN      
        1 mqverrealw arslefemra adqsrlnhrd gcllemrark rletelprle eeiktyisrh
       61 tgreeqflse saidflehlc sphdaynlek drerqervrt f
//
LOCUS       XP_077502830             121 aa            linear   INV 25-NOV-2025
DEFINITION  protein regulator of cytokinesis 1-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502830
VERSION     XP_077502830.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646704.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..121
                     /product="protein regulator of cytokinesis 1-like isoform
                     X1"
                     /calculated_mol_wt=14296
     Region          <3..120
                     /region_name="MAP65_ASE1"
                     /note="Microtubule associated protein (MAP65/ASE1 family);
                     pfam03999"
                     /db_xref="CDD:427641"
     CDS             1..121
                     /gene="LOC144113564"
                     /coded_by="XM_077646704.1:358..723"
                     /db_xref="GeneID:144113564"
ORIGIN      
        1 mqverrealw arflefekra adqshlnhrg gcllemrark rletelprle eeiktyisrh
       61 tgreeqflse saidflehlc sphdaynlek drerqertrg lknrrknseg srsstpntsp
      121 f
//
LOCUS       XP_077502831             104 aa            linear   INV 25-NOV-2025
DEFINITION  protein regulator of cytokinesis 1-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502831
VERSION     XP_077502831.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646705.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..104
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..104
                     /product="protein regulator of cytokinesis 1-like isoform
                     X2"
                     /calculated_mol_wt=12140
     Region          <3..103
                     /region_name="MAP65_ASE1"
                     /note="Microtubule associated protein (MAP65/ASE1 family);
                     pfam03999"
                     /db_xref="CDD:427641"
     CDS             1..104
                     /gene="LOC144113564"
                     /coded_by="XM_077646705.1:44..358"
                     /db_xref="GeneID:144113564"
ORIGIN      
        1 mraadqsrln hrggcllemr arkrletelp rleeeiktyi srhtgreeqf lsesaidfle
       61 hlcsphdayn lekdrerqer trglknrrkn segsrsstpk tspf
//
LOCUS       XP_077502832             151 aa            linear   INV 25-NOV-2025
DEFINITION  protein O-mannosyl-transferase Tmtc3-like [Amblyomma americanum].
ACCESSION   XP_077502832
VERSION     XP_077502832.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646706.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..151
                     /product="protein O-mannosyl-transferase Tmtc3-like"
                     /calculated_mol_wt=16090
     Region          2..>93
                     /region_name="Spy"
                     /note="Predicted O-linked N-acetylglucosamine transferase,
                     SPINDLY family [Posttranslational modification, protein
                     turnover, chaperones]; COG3914"
                     /db_xref="CDD:443119"
     Region          17..48
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          53..79
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     CDS             1..151
                     /gene="LOC144113565"
                     /coded_by="XM_077646706.1:196..651"
                     /db_xref="GeneID:144113565"
ORIGIN      
        1 meamrflkdl lkyhpnhikg lvllgdinvn hlkdleaaee cyrrilaldp gnvqgshnlc
       61 vvyvergels raercfkhat tldpnatyir ehlqvtrtll rnrsgdaaag gerrsgagal
      121 tgsppqpppv lqpfaaaaaa ssaaasaana q
//
LOCUS       XP_077502834             675 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113567 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502834
VERSION     XP_077502834.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646708.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..675
                     /product="uncharacterized protein LOC144113567 isoform X1"
                     /calculated_mol_wt=70216
     Region          15..72
                     /region_name="SSXT"
                     /note="SSXT protein (N-terminal region); pfam05030"
                     /db_xref="CDD:428268"
     Region          <97..658
                     /region_name="Glutenin_hmw"
                     /note="High molecular weight glutenin subunit; pfam03157"
                     /db_xref="CDD:367362"
     Region          <181..>329
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     CDS             1..675
                     /gene="LOC144113567"
                     /coded_by="XM_077646708.1:151..2178"
                     /db_xref="GeneID:144113567"
ORIGIN      
        1 msvafaqqqk grhnpspaqi qkmldensql iqaivdyqnk gkaseclqyq qilhrnlvyl
       61 asvadagpvv qasipppcgp ggpdqgggpa pgsllpsqqs ygsamqsqqq papggpgvgq
      121 qppmgghgms gygrsgppps snmvsqqqpm paqqphhqpr wsyrhwctfp prggatghgp
      181 mqhqpssqqh pamnqhsqqp hmgqqpgaqh mtqppshpsa mqpvmgsahq pgqahqmsqh
      241 gaspqgpvqq qmqspqqhss qhpgqhqgqh pgqhpgqphp gqhpgqhpgq hpvqhpghps
      301 qhqgqhpgqh pvqhpsqhsv qhsvqhpgqh pgqhpgqhpg phpsqhpgqq pqqphpgqph
      361 pgqhpgqphs sqnqgqhlsq hpsqhpvphp aphpaqhqsq hsgqpqqppq qqhaspppvs
      421 sppsgpgtpq rqgpsppsqq hhppygsqgs lnppnrsspt pnqspapass lstqnyaqhs
      481 shgqqgaalg pqqhqpsggp asspyggysp qvgpsqtppq qrppsqpspa ttphpsspyp
      541 ghkmaaqsgy patpyqaags pgfpgappgg aagaappagt ypattsasag yaqygqgypq
      601 gygvaygygp sggypqggay lgaypqsgtg aplrypagyp gyghyrapqp tgqqaaayga
      661 yeqqgyppga ysqaq
//
LOCUS       XP_077502835             652 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113567 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502835
VERSION     XP_077502835.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646709.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..652
                     /product="uncharacterized protein LOC144113567 isoform X2"
                     /calculated_mol_wt=67548
     Region          15..72
                     /region_name="SSXT"
                     /note="SSXT protein (N-terminal region); pfam05030"
                     /db_xref="CDD:428268"
     Region          174..>361
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     Region          <410..626
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     CDS             1..652
                     /gene="LOC144113567"
                     /coded_by="XM_077646709.1:153..2111"
                     /db_xref="GeneID:144113567"
ORIGIN      
        1 msvafaqqqk grhnpspaqi qkmldensql iqaivdyqnk gkaseclqyq qilhrnlvyl
       61 asvadagpvv qasipppcgp ggpdqgggpa pgsllpsqqs ygsamqsqqq papggpgvgq
      121 qppmgghgms gygrsgppps snmvsqqqpm paqqphhqph qpssqqhpam nqhsqqphmg
      181 qqpgaqhmtq ppshpsamqp vmgsahqpgq ahqmsqhgas pqgpvqqqmq spqqhssqhp
      241 gqhqgqhpgq hpgqphpgqh pgqhpgqhpv qhpghpsqhq gqhpgqhpvq hpsqhsvqhs
      301 vqhpgqhpgq hpgqhpgphp sqhpgqqpqq phpgqphpgq hpgqphssqn qgqhlsqhps
      361 qhpvphpaph paqhqsqhsg qpqqppqqqh aspppvsspp sgpgtpqrqg psppsqqhhp
      421 pygsqgslnp pnrssptpnq spapasslst qnyaqhsshg qqgaalgpqq hqpsggpass
      481 pyggyspqvg psqtppqqrp psqpspattp hpsspypghk maaqsgypat pyqaagspgf
      541 pgappggaag aappagtypa ttsasagyaq ygqgypqgyg vaygygpsgg ypqggaylga
      601 ypqsgtgapl rypagypgyg hyrapqptgq qaaaygayeq qgyppgaysq aq
//
LOCUS       XP_077502836             655 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113567 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502836
VERSION     XP_077502836.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646710.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..655
                     /product="uncharacterized protein LOC144113567 isoform X3"
                     /calculated_mol_wt=67905
     Region          15..72
                     /region_name="SSXT"
                     /note="SSXT protein (N-terminal region); pfam05030"
                     /db_xref="CDD:428268"
     Region          <97..638
                     /region_name="Glutenin_hmw"
                     /note="High molecular weight glutenin subunit; pfam03157"
                     /db_xref="CDD:367362"
     Region          <161..>309
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     CDS             1..655
                     /gene="LOC144113567"
                     /coded_by="XM_077646710.1:152..2119"
                     /db_xref="GeneID:144113567"
ORIGIN      
        1 msvafaqqqk grhnpspaqi qkmldensql iqaivdyqnk gkaseclqyq qilhrnlvyl
       61 asvadagpvv qasipppcgp ggpdqgggpa pgsllpsqqs ygsamqsqqq papggpgvgq
      121 qppmgghgms gygrsgppps snmvsqqqpm paqqphhqpp mqhqpssqqh pamnqhsqqp
      181 hmgqqpgaqh mtqppshpsa mqpvmgsahq pgqahqmsqh gaspqgpvqq qmqspqqhss
      241 qhpgqhqgqh pgqhpgqphp gqhpgqhpgq hpvqhpghps qhqgqhpgqh pvqhpsqhsv
      301 qhsvqhpgqh pgqhpgqhpg phpsqhpgqq pqqphpgqph pgqhpgqphs sqnqgqhlsq
      361 hpsqhpvphp aphpaqhqsq hsgqpqqppq qqhaspppvs sppsgpgtpq rqgpsppsqq
      421 hhppygsqgs lnppnrsspt pnqspapass lstqnyaqhs shgqqgaalg pqqhqpsggp
      481 asspyggysp qvgpsqtppq qrppsqpspa ttphpsspyp ghkmaaqsgy patpyqaags
      541 pgfpgappgg aagaappagt ypattsasag yaqygqgypq gygvaygygp sggypqggay
      601 lgaypqsgtg aplrypagyp gyghyrapqp tgqqaaayga yeqqgyppga ysqaq
//
LOCUS       XP_077502837             323 aa            linear   INV 25-NOV-2025
DEFINITION  acyl-coenzyme A diphosphatase Fitm [Amblyomma americanum].
ACCESSION   XP_077502837
VERSION     XP_077502837.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646711.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..323
                     /product="acyl-coenzyme A diphosphatase Fitm"
                     /calculated_mol_wt=36600
     Region          84..291
                     /region_name="Scs3p"
                     /note="Inositol phospholipid synthesis and
                     fat-storage-inducing TM; pfam10261"
                     /db_xref="CDD:402051"
     CDS             1..323
                     /gene="Fitm"
                     /coded_by="XM_077646711.1:49..1020"
                     /db_xref="GeneID:144113568"
ORIGIN      
        1 magnqrrskv sgamkwdatp akqtttgrkp laepmsvkqv fvmvglhlcr rvvvveprvk
       61 vplylgvlli gsvmcdffpi prtylsrkdn vfnayfvkla wgwtlvtvgl fvavsswvyc
      121 cgnralvvrh ltrlavgtaa wffttnsfva fetytsrctl ekhgtrdacl kagqrwfgfd
      181 isghafllif cnlliaeear sfcgwerigd llrnekyddd salkdlpaeq lrllqeaypr
      241 ltpyvrllfv amtyfsllwd lmlictvvyf hsmpqkvlgg vvaivewfml yrvwyvswsp
      301 glpgkgpfky adaprkksqg aqn
//
LOCUS       XP_077502838             184 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113569 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502838
VERSION     XP_077502838.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646712.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..184
                     /product="uncharacterized protein LOC144113569 isoform X1"
                     /calculated_mol_wt=20763
     Region          4..57
                     /region_name="CD_CSD"
                     /note="CHROMO (CHRromatin Organization Modifier) domains
                     and chromo shadow domains; cd00024"
                     /db_xref="CDD:349274"
     Site            order(4..8,27,29..32,36,38..41,43..44,51,54..55)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349274"
     CDS             1..184
                     /gene="LOC144113569"
                     /coded_by="XM_077646712.1:148..702"
                     /db_xref="GeneID:144113569"
ORIGIN      
        1 mnafeveril drrtrrkgrr kileyrirwq nfgpsedtwe prenlrgcpe llrefednla
       61 qmqeadhmdv ssttdnfggs dseeergyss ddddstelmp aspnmplgpa qhkkhrrpag
      121 lplpwsatrw tvvegsgsvr vrgqnahvha tdlrkntrst tllplpqpal vaelmqsktv
      181 avac
//
LOCUS       XP_077502839             169 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113569 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502839
VERSION     XP_077502839.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646713.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..169
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..169
                     /product="uncharacterized protein LOC144113569 isoform X2"
                     /calculated_mol_wt=19361
     Region          4..57
                     /region_name="CD_CSD"
                     /note="CHROMO (CHRromatin Organization Modifier) domains
                     and chromo shadow domains; cd00024"
                     /db_xref="CDD:349274"
     Site            order(4..8,27,29..32,36,38..41,43..44,51,54..55)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349274"
     CDS             1..169
                     /gene="LOC144113569"
                     /coded_by="XM_077646713.1:148..657"
                     /db_xref="GeneID:144113569"
ORIGIN      
        1 mnafeveril drrtrrkgrr kileyrirwq nfgpsedtwe prenlrgcpe llrefednla
       61 qmqeadhmdv ssttdnfggs dseeergyss ddddstelmp aspnmplgpa qhdheevaaa
      121 retahgrnva aestlffavl lcvlvvffya ttdvkifrgg wkwlseifi
//
LOCUS       XP_077502840             144 aa            linear   INV 25-NOV-2025
DEFINITION  chromobox protein homolog 1-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077502840
VERSION     XP_077502840.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646714.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..144
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..144
                     /product="chromobox protein homolog 1-like isoform X3"
                     /calculated_mol_wt=16703
     Region          4..57
                     /region_name="CD_CSD"
                     /note="CHROMO (CHRromatin Organization Modifier) domains
                     and chromo shadow domains; cd00024"
                     /db_xref="CDD:349274"
     Site            order(4..8,27,29..32,36,38..41,43..44,51,54..55)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349274"
     CDS             1..144
                     /gene="LOC144113569"
                     /coded_by="XM_077646714.1:148..582"
                     /db_xref="GeneID:144113569"
ORIGIN      
        1 mnafeveril drrtrrkgrr kileyrirwq nfgpsedtwe prenlrgcpe llrefednla
       61 qmqeadhmdv ssttdnfggs dseeergdhe evaaaretah grnvaaestl ffavllcvlv
      121 vffyattdvk ifrggwkwls eifi
//
LOCUS       XP_077502841             475 aa            linear   INV 25-NOV-2025
DEFINITION  oxidative stress-induced growth inhibitor 1-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077502841
VERSION     XP_077502841.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646715.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..475
                     /product="oxidative stress-induced growth inhibitor 1-like
                     isoform X1"
                     /calculated_mol_wt=52134
     Region          162..405
                     /region_name="NADB_Rossmann"
                     /note="Rossmann-fold NAD(P)(+)-binding proteins; cl21454"
                     /db_xref="CDD:473865"
     CDS             1..475
                     /gene="LOC144113570"
                     /coded_by="XM_077646715.1:464..1891"
                     /db_xref="GeneID:144113570"
ORIGIN      
        1 marlaadhqr dratahdqei revpanmaiy kdvvvigngp sgitmsyfla gnwpyyapgt
       61 chpnpylhar lednrslplv eqdlsllssg legrscnpvs llldtlchpd adlgsdepsa
      121 ltwvhdpska ldhivlgngl pggswqrmdg giltislgsw melpdltfke wdsskpqppq
      181 dspncqnrvs vqrvaqyyrd yvdlkglqhy fqnfacvqsv rpiddpeedh lwevkgmnkq
      241 tgtsfcyitp hvvlaaghyd aprklgvpge dlpfvshdlg qlevllrdtk ssitklavvg
      301 sglsaadavi aarfhgvdvc hvfrkkvddp elvfnqlprs mypeyhkvhq mmssaehypg
      361 ykayahcqvr cihadgrmel dsgteiqdis hvlvligshp nldflpsagq qlglvagllv
      421 dcranpiqid pythetvalk gvyalgplvg dnfvrflqgg slavtahiwr glggt
//
LOCUS       XP_077502842             475 aa            linear   INV 25-NOV-2025
DEFINITION  oxidative stress-induced growth inhibitor 1-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077502842
VERSION     XP_077502842.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646716.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..475
                     /product="oxidative stress-induced growth inhibitor 1-like
                     isoform X1"
                     /calculated_mol_wt=52134
     Region          162..405
                     /region_name="NADB_Rossmann"
                     /note="Rossmann-fold NAD(P)(+)-binding proteins; cl21454"
                     /db_xref="CDD:473865"
     CDS             1..475
                     /gene="LOC144113570"
                     /coded_by="XM_077646716.1:398..1825"
                     /db_xref="GeneID:144113570"
ORIGIN      
        1 marlaadhqr dratahdqei revpanmaiy kdvvvigngp sgitmsyfla gnwpyyapgt
       61 chpnpylhar lednrslplv eqdlsllssg legrscnpvs llldtlchpd adlgsdepsa
      121 ltwvhdpska ldhivlgngl pggswqrmdg giltislgsw melpdltfke wdsskpqppq
      181 dspncqnrvs vqrvaqyyrd yvdlkglqhy fqnfacvqsv rpiddpeedh lwevkgmnkq
      241 tgtsfcyitp hvvlaaghyd aprklgvpge dlpfvshdlg qlevllrdtk ssitklavvg
      301 sglsaadavi aarfhgvdvc hvfrkkvddp elvfnqlprs mypeyhkvhq mmssaehypg
      361 ykayahcqvr cihadgrmel dsgteiqdis hvlvligshp nldflpsagq qlglvagllv
      421 dcranpiqid pythetvalk gvyalgplvg dnfvrflqgg slavtahiwr glggt
//
LOCUS       XP_077502843             475 aa            linear   INV 25-NOV-2025
DEFINITION  oxidative stress-induced growth inhibitor 1-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077502843
VERSION     XP_077502843.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646717.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..475
                     /product="oxidative stress-induced growth inhibitor 1-like
                     isoform X1"
                     /calculated_mol_wt=52134
     Region          162..405
                     /region_name="NADB_Rossmann"
                     /note="Rossmann-fold NAD(P)(+)-binding proteins; cl21454"
                     /db_xref="CDD:473865"
     CDS             1..475
                     /gene="LOC144113570"
                     /coded_by="XM_077646717.1:193..1620"
                     /db_xref="GeneID:144113570"
ORIGIN      
        1 marlaadhqr dratahdqei revpanmaiy kdvvvigngp sgitmsyfla gnwpyyapgt
       61 chpnpylhar lednrslplv eqdlsllssg legrscnpvs llldtlchpd adlgsdepsa
      121 ltwvhdpska ldhivlgngl pggswqrmdg giltislgsw melpdltfke wdsskpqppq
      181 dspncqnrvs vqrvaqyyrd yvdlkglqhy fqnfacvqsv rpiddpeedh lwevkgmnkq
      241 tgtsfcyitp hvvlaaghyd aprklgvpge dlpfvshdlg qlevllrdtk ssitklavvg
      301 sglsaadavi aarfhgvdvc hvfrkkvddp elvfnqlprs mypeyhkvhq mmssaehypg
      361 ykayahcqvr cihadgrmel dsgteiqdis hvlvligshp nldflpsagq qlglvagllv
      421 dcranpiqid pythetvalk gvyalgplvg dnfvrflqgg slavtahiwr glggt
//
LOCUS       XP_077502844             475 aa            linear   INV 25-NOV-2025
DEFINITION  oxidative stress-induced growth inhibitor 1-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077502844
VERSION     XP_077502844.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646718.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..475
                     /product="oxidative stress-induced growth inhibitor 1-like
                     isoform X1"
                     /calculated_mol_wt=52134
     Region          162..405
                     /region_name="NADB_Rossmann"
                     /note="Rossmann-fold NAD(P)(+)-binding proteins; cl21454"
                     /db_xref="CDD:473865"
     CDS             1..475
                     /gene="LOC144113570"
                     /coded_by="XM_077646718.1:149..1576"
                     /db_xref="GeneID:144113570"
ORIGIN      
        1 marlaadhqr dratahdqei revpanmaiy kdvvvigngp sgitmsyfla gnwpyyapgt
       61 chpnpylhar lednrslplv eqdlsllssg legrscnpvs llldtlchpd adlgsdepsa
      121 ltwvhdpska ldhivlgngl pggswqrmdg giltislgsw melpdltfke wdsskpqppq
      181 dspncqnrvs vqrvaqyyrd yvdlkglqhy fqnfacvqsv rpiddpeedh lwevkgmnkq
      241 tgtsfcyitp hvvlaaghyd aprklgvpge dlpfvshdlg qlevllrdtk ssitklavvg
      301 sglsaadavi aarfhgvdvc hvfrkkvddp elvfnqlprs mypeyhkvhq mmssaehypg
      361 ykayahcqvr cihadgrmel dsgteiqdis hvlvligshp nldflpsagq qlglvagllv
      421 dcranpiqid pythetvalk gvyalgplvg dnfvrflqgg slavtahiwr glggt
//
LOCUS       XP_077502845             475 aa            linear   INV 25-NOV-2025
DEFINITION  oxidative stress-induced growth inhibitor 1-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077502845
VERSION     XP_077502845.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646719.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..475
                     /product="oxidative stress-induced growth inhibitor 1-like
                     isoform X1"
                     /calculated_mol_wt=52134
     Region          162..405
                     /region_name="NADB_Rossmann"
                     /note="Rossmann-fold NAD(P)(+)-binding proteins; cl21454"
                     /db_xref="CDD:473865"
     CDS             1..475
                     /gene="LOC144113570"
                     /coded_by="XM_077646719.1:84..1511"
                     /db_xref="GeneID:144113570"
ORIGIN      
        1 marlaadhqr dratahdqei revpanmaiy kdvvvigngp sgitmsyfla gnwpyyapgt
       61 chpnpylhar lednrslplv eqdlsllssg legrscnpvs llldtlchpd adlgsdepsa
      121 ltwvhdpska ldhivlgngl pggswqrmdg giltislgsw melpdltfke wdsskpqppq
      181 dspncqnrvs vqrvaqyyrd yvdlkglqhy fqnfacvqsv rpiddpeedh lwevkgmnkq
      241 tgtsfcyitp hvvlaaghyd aprklgvpge dlpfvshdlg qlevllrdtk ssitklavvg
      301 sglsaadavi aarfhgvdvc hvfrkkvddp elvfnqlprs mypeyhkvhq mmssaehypg
      361 ykayahcqvr cihadgrmel dsgteiqdis hvlvligshp nldflpsagq qlglvagllv
      421 dcranpiqid pythetvalk gvyalgplvg dnfvrflqgg slavtahiwr glggt
//
LOCUS       XP_077502846             464 aa            linear   INV 25-NOV-2025
DEFINITION  oxidative stress-induced growth inhibitor 1-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077502846
VERSION     XP_077502846.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646720.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..464
                     /product="oxidative stress-induced growth inhibitor 1-like
                     isoform X2"
                     /calculated_mol_wt=51077
     Region          151..394
                     /region_name="NADB_Rossmann"
                     /note="Rossmann-fold NAD(P)(+)-binding proteins; cl21454"
                     /db_xref="CDD:473865"
     CDS             1..464
                     /gene="LOC144113570"
                     /coded_by="XM_077646720.1:81..1475"
                     /db_xref="GeneID:144113570"
ORIGIN      
        1 mrrrrmteir evpanmaiyk dvvvigngps gitmsyflag nwpyyapgtc hpnpylharl
       61 ednrslplve qdlsllssgl egrscnpvsl lldtlchpda dlgsdepsal twvhdpskal
      121 dhivlgnglp ggswqrmdgg iltislgswm elpdltfkew dsskpqppqd spncqnrvsv
      181 qrvaqyyrdy vdlkglqhyf qnfacvqsvr piddpeedhl wevkgmnkqt gtsfcyitph
      241 vvlaaghyda prklgvpged lpfvshdlgq levllrdtks sitklavvgs glsaadavia
      301 arfhgvdvch vfrkkvddpe lvfnqlprsm ypeyhkvhqm mssaehypgy kayahcqvrc
      361 ihadgrmeld sgteiqdish vlvligshpn ldflpsagqq lglvagllvd cranpiqidp
      421 ythetvalkg vyalgplvgd nfvrflqggs lavtahiwrg lggt
//
LOCUS       XP_077502847             449 aa            linear   INV 25-NOV-2025
DEFINITION  oxidative stress-induced growth inhibitor 1-like isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077502847
VERSION     XP_077502847.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646721.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..449
                     /product="oxidative stress-induced growth inhibitor 1-like
                     isoform X3"
                     /calculated_mol_wt=49180
     Region          136..379
                     /region_name="NADB_Rossmann"
                     /note="Rossmann-fold NAD(P)(+)-binding proteins; cl21454"
                     /db_xref="CDD:473865"
     CDS             1..449
                     /gene="LOC144113570"
                     /coded_by="XM_077646721.1:398..1747"
                     /db_xref="GeneID:144113570"
ORIGIN      
        1 maiykdvvvi gngpsgitms yflagnwpyy apgtchpnpy lharlednrs lplveqdlsl
       61 lssglegrsc npvsllldtl chpdadlgsd epsaltwvhd pskaldhivl gnglpggswq
      121 rmdggiltis lgswmelpdl tfkewdsskp qppqdspncq nrvsvqrvaq yyrdyvdlkg
      181 lqhyfqnfac vqsvrpiddp eedhlwevkg mnkqtgtsfc yitphvvlaa ghydaprklg
      241 vpgedlpfvs hdlgqlevll rdtkssitkl avvgsglsaa daviaarfhg vdvchvfrkk
      301 vddpelvfnq lprsmypeyh kvhqmmssae hypgykayah cqvrcihadg rmeldsgtei
      361 qdishvlvli gshpnldflp sagqqlglva gllvdcranp iqidpythet valkgvyalg
      421 plvgdnfvrf lqggslavta hiwrglggt
//
LOCUS       XP_077502848             449 aa            linear   INV 25-NOV-2025
DEFINITION  oxidative stress-induced growth inhibitor 1-like isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077502848
VERSION     XP_077502848.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646722.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..449
                     /product="oxidative stress-induced growth inhibitor 1-like
                     isoform X3"
                     /calculated_mol_wt=49180
     Region          136..379
                     /region_name="NADB_Rossmann"
                     /note="Rossmann-fold NAD(P)(+)-binding proteins; cl21454"
                     /db_xref="CDD:473865"
     CDS             1..449
                     /gene="LOC144113570"
                     /coded_by="XM_077646722.1:127..1476"
                     /db_xref="GeneID:144113570"
ORIGIN      
        1 maiykdvvvi gngpsgitms yflagnwpyy apgtchpnpy lharlednrs lplveqdlsl
       61 lssglegrsc npvsllldtl chpdadlgsd epsaltwvhd pskaldhivl gnglpggswq
      121 rmdggiltis lgswmelpdl tfkewdsskp qppqdspncq nrvsvqrvaq yyrdyvdlkg
      181 lqhyfqnfac vqsvrpiddp eedhlwevkg mnkqtgtsfc yitphvvlaa ghydaprklg
      241 vpgedlpfvs hdlgqlevll rdtkssitkl avvgsglsaa daviaarfhg vdvchvfrkk
      301 vddpelvfnq lprsmypeyh kvhqmmssae hypgykayah cqvrcihadg rmeldsgtei
      361 qdishvlvli gshpnldflp sagqqlglva gllvdcranp iqidpythet valkgvyalg
      421 plvgdnfvrf lqggslavta hiwrglggt
//
LOCUS       XP_077502849             655 aa            linear   INV 25-NOV-2025
DEFINITION  ATP-binding cassette sub-family B member 10, mitochondrial-like
            [Amblyomma americanum].
ACCESSION   XP_077502849
VERSION     XP_077502849.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646723.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..655
                     /product="ATP-binding cassette sub-family B member 10,
                     mitochondrial-like"
                     /calculated_mol_wt=70979
     Region          72..651
                     /region_name="MdlB"
                     /note="ABC-type multidrug transport system, ATPase and
                     permease component [Defense mechanisms]; COG1132"
                     /db_xref="CDD:440747"
     CDS             1..655
                     /gene="LOC144113572"
                     /coded_by="XM_077646723.1:242..2209"
                     /db_xref="GeneID:144113572"
ORIGIN      
        1 mhayilgvgs plfrqvrvrk hllpkclstv ngavrcssrn vlrlctpppk pvvsavlcrt
       61 csgsrhtdss elkrllrqak perfrlgaav illfvsssvm iafpfcigkv idviytapnh
      121 eelrenlswi crlltgvvvi ggvanfgrvy lmntsaqrii nalrkqahas lmqqevaffd
      181 rnrtgdlitr lssdtalvgm sltqnmsdgl rsavavlggv gmmlytspql slvglsivpp
      241 valmsfafar rlrqvaadvq trlaqssaia eeqlsnirtv rafakesyek nryaeglsrl
      301 lemvnsetwl kavffgctga tgnmivlavl yyggvlmsdg rltvgnlssf llyaayvgvs
      361 igglggffte ttkalgasrk vweiadrvpa lpnsgglilp nlqgqvefsn vtfaypsrpd
      421 itvlknlnls vpagsvlaiv gpsgqgkstl aslllrlydp shgvvmldgi dirkldpsfl
      481 rlhvgivsqe ptlfattifd nilygaksle rstkddvmra aseanalefi qglpdgfntm
      541 vgergvllsg gqkqriaiar ailrdpsili ldeatsslda vseravqeal kklmmgrtvl
      601 tiahrlstir radrivvlka gtivesgtyd qlmeitdglf rklvehqihd eitsg
//
LOCUS       XP_077502850             240 aa            linear   INV 25-NOV-2025
DEFINITION  type I phosphatidylinositol 4,5-bisphosphate 4-phosphatase-B
            [Amblyomma americanum].
ACCESSION   XP_077502850
VERSION     XP_077502850.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646724.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..240
                     /product="type I phosphatidylinositol 4,5-bisphosphate
                     4-phosphatase-B"
                     /calculated_mol_wt=26533
     Region          9..234
                     /region_name="Tmemb_55A"
                     /note="Transmembrane protein 55A; pfam09788"
                     /db_xref="CDD:462901"
     CDS             1..240
                     /gene="LOC144113573"
                     /coded_by="XM_077646724.1:108..830"
                     /db_xref="GeneID:144113573"
ORIGIN      
        1 mdlrdesaer tpllqeqsds sfpsynvqar delpppystq yaaqgnivpc rvcgnvidit
       61 nrrdqhvvkc shcneatpir happgkkyvr cpcncllicn vtsqrigcpr anchriinla
      121 psttnippas vpglcrvscv hchqtflfnt lnnalsrcph crkvssvgpq fartrgilsl
      181 vlalvilait lgitlgtyky aeekkgiyvv yacglvafll fllrslyyft mrvshieepa
//
LOCUS       XP_077502851             255 aa            linear   INV 25-NOV-2025
DEFINITION  peptide methionine sulfoxide reductase-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502851
VERSION     XP_077502851.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646725.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..255
                     /product="peptide methionine sulfoxide reductase-like
                     isoform X1"
                     /calculated_mol_wt=28895
     Region          45..186
                     /region_name="PMSR"
                     /note="Peptide methionine sulfoxide reductase; pfam01625"
                     /db_xref="CDD:460270"
     CDS             1..255
                     /gene="LOC144113574"
                     /coded_by="XM_077646725.1:205..972"
                     /db_xref="GeneID:144113574"
ORIGIN      
        1 mgllaalate lymstckwyd riinfrtsiq pstkrlyepe vpfekatfgm gcfwapeamf
       61 gaakgvlrtt vgfaaaakke sityrslspg eeeedeadht evtrveydpr etnfkellkl
      121 fwvhhdptrq rgtkeymsai fchddvqete akqsleehrk nfrnpivtki lkleqfytae
      181 ehhqkyrlqr ktalyaslvq sgmkdvsrsh vatrlngyvg gfgtlaafda esgrlglsad
      241 qinavrtlir rggar
//
LOCUS       XP_077502852             246 aa            linear   INV 25-NOV-2025
DEFINITION  peptide methionine sulfoxide reductase-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502852
VERSION     XP_077502852.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646726.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..246
                     /product="peptide methionine sulfoxide reductase-like
                     isoform X2"
                     /calculated_mol_wt=27893
     Region          45..177
                     /region_name="PMSR"
                     /note="Peptide methionine sulfoxide reductase; pfam01625"
                     /db_xref="CDD:460270"
     CDS             1..246
                     /gene="LOC144113574"
                     /coded_by="XM_077646726.1:186..926"
                     /db_xref="GeneID:144113574"
ORIGIN      
        1 mgllaalate lymstckwyd riinfrtsiq pstkrlyepe vpfekatfgm gcfwapeamf
       61 gaakgvlrtt vgfaaaakke sityrsladh tevtrveydp retnfkellk lfwvhhdptr
      121 qrgtkeymsa ifchddvqet eakqsleehr knfrnpivtk ilkleqfyta eehhqkyrlq
      181 rktalyaslv qsgmkdvsrs hvatrlngyv ggfgtlaafd aesgrlglsa dqinavrtli
      241 rrggar
//
LOCUS       XP_077502853             234 aa            linear   INV 25-NOV-2025
DEFINITION  peptide methionine sulfoxide reductase-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502853
VERSION     XP_077502853.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646727.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..234
                     /product="peptide methionine sulfoxide reductase-like
                     isoform X3"
                     /calculated_mol_wt=26192
     Region          24..165
                     /region_name="PMSR"
                     /note="Peptide methionine sulfoxide reductase; pfam01625"
                     /db_xref="CDD:460270"
     CDS             1..234
                     /gene="LOC144113574"
                     /coded_by="XM_077646727.1:195..899"
                     /db_xref="GeneID:144113574"
ORIGIN      
        1 mgsgsgaiqp stkrlyepev pfekatfgmg cfwapeamfg aakgvlrttv gfaaaakkes
       61 ityrslspge eeedeadhte vtrveydpre tnfkellklf wvhhdptrqr gtkeymsaif
      121 chddvqetea kqsleehrkn frnpivtkil kleqfytaee hhqkyrlqrk talyaslvqs
      181 gmkdvsrshv atrlngyvgg fgtlaafdae sgrlglsadq inavrtlirr ggar
//
LOCUS       XP_077502854             181 aa            linear   INV 25-NOV-2025
DEFINITION  protein FAM210B, mitochondrial-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502854
VERSION     XP_077502854.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646728.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="protein FAM210B, mitochondrial-like isoform X1"
                     /calculated_mol_wt=19506
     Region          77..166
                     /region_name="FAM210A-B_dom"
                     /note="FAM210A/B-like domain; pfam06916"
                     /db_xref="CDD:429191"
     CDS             1..181
                     /gene="LOC144113575"
                     /coded_by="XM_077646728.1:135..680"
                     /db_xref="GeneID:144113575"
ORIGIN      
        1 mlsvasfcaq qrilvscsqa lrsscsvfrs tsskhigkpl llhvpapfss alsalhrcsa
       61 lsqgdtgqks apvsrrekwk haikeygsam vafhvvlsit sfgicyvlvs sgidmtaiae
      121 rlgfnmdsew vnskvaggsg tlviayavhk lftpvrmgit ltaapilvrw lrrngifgfr
      181 y
//
LOCUS       XP_077502855             130 aa            linear   INV 25-NOV-2025
DEFINITION  protein FAM210B, mitochondrial-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502855
VERSION     XP_077502855.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646729.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..130
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..130
                     /product="protein FAM210B, mitochondrial-like isoform X2"
                     /calculated_mol_wt=13951
     Region          26..115
                     /region_name="FAM210A-B_dom"
                     /note="FAM210A/B-like domain; pfam06916"
                     /db_xref="CDD:429191"
     CDS             1..130
                     /gene="LOC144113575"
                     /coded_by="XM_077646729.1:367..759"
                     /db_xref="GeneID:144113575"
ORIGIN      
        1 mgshaasssv sqgdtgqksa pvsrrekwkh aikeygsamv afhvvlsits fgicyvlvss
       61 gidmtaiaer lgfnmdsewv nskvaggsgt lviayavhkl ftpvrmgitl taapilvrwl
      121 rrngifgfry
//
LOCUS       XP_077502856             130 aa            linear   INV 25-NOV-2025
DEFINITION  protein FAM210B, mitochondrial-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502856
VERSION     XP_077502856.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646730.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..130
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..130
                     /product="protein FAM210B, mitochondrial-like isoform X2"
                     /calculated_mol_wt=13951
     Region          26..115
                     /region_name="FAM210A-B_dom"
                     /note="FAM210A/B-like domain; pfam06916"
                     /db_xref="CDD:429191"
     CDS             1..130
                     /gene="LOC144113575"
                     /coded_by="XM_077646730.1:293..685"
                     /db_xref="GeneID:144113575"
ORIGIN      
        1 mgshaasssv sqgdtgqksa pvsrrekwkh aikeygsamv afhvvlsits fgicyvlvss
       61 gidmtaiaer lgfnmdsewv nskvaggsgt lviayavhkl ftpvrmgitl taapilvrwl
      121 rrngifgfry
//
LOCUS       XP_077502857             606 aa            linear   INV 25-NOV-2025
DEFINITION  LIM domain-containing protein jub [Amblyomma americanum].
ACCESSION   XP_077502857
VERSION     XP_077502857.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646731.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..606
                     /product="LIM domain-containing protein jub"
                     /calculated_mol_wt=65754
     Region          388..441
                     /region_name="LIM1_Ajuba_like"
                     /note="The first LIM domain of Ajuba-like proteins;
                     cd09352"
                     /db_xref="CDD:188738"
     Site            order(388,391,410,413,416,419,437,440)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188738"
     Region          453..505
                     /region_name="LIM2_Ajuba_like"
                     /note="The second LIM domain of Ajuba-like proteins;
                     cd09355"
                     /db_xref="CDD:188741"
     Site            order(453,456,473,476,479,482,501,504)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188741"
     Region          513..574
                     /region_name="LIM3_Ajuba_like"
                     /note="The third LIM domain of Ajuba-like proteins;
                     cd09438"
                     /db_xref="CDD:188822"
     Site            order(513,516,540,543,546,549,570,573)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188822"
     CDS             1..606
                     /gene="jub"
                     /coded_by="XM_077646731.1:228..2048"
                     /db_xref="GeneID:144113577"
ORIGIN      
        1 myddelasda trilqdlslf evptrdhlta atpkrtdtsa gedfekkrei yanlnsksyp
       61 dfalagdhdy grivsraats qgsyasadvl gsprrylpts rralgaiesl adgkelpvya
      121 ridrasviaa skfatpkapc tevasgvppp dyarwaackt rlpdsahssp rssvssssrd
      181 sqhsnssaga apiyenvaal qsprsslssq dskppspras hcepppyphg kpppeypiya
      241 nldgsslqrn glvrpcaqkp ssyassvrys tagnnslscs ypigkqqqvf qmrsqpcena
      301 aglaarspqa acpqvppqap cpqtlppppp ypgykpntln tktllpynvt pprpkgptea
      361 ekkiealmrq iedeleqnpp egeffgicht cgekvtgagq acqamgnlyh tncficcscg
      421 ralrgkafyn vhgkvyceed ylysgfqqta ekcavcghli memilqamgk syhpgcfrcc
      481 icnecldgvp ftidmdnkiy cvndyhkmfa pkcaacgkai tpvegtdetv rvvsmdkdfh
      541 vdcyvceecs lqltddrdkr cypldghllc qgchvrrlqa qgsaargepp pqgaqaqivw
      601 ngaqlh
//
LOCUS       XP_077502858             137 aa            linear   INV 25-NOV-2025
DEFINITION  prefoldin subunit 4 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502858
VERSION     XP_077502858.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646732.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..137
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..137
                     /product="prefoldin subunit 4 isoform X1"
                     /calculated_mol_wt=15523
     Region          23..125
                     /region_name="Prefoldin_4"
                     /note="prefoldin subunit 4; cd23165"
                     /db_xref="CDD:467481"
     Site            order(23,25..28,31..32,35,102,105..106,108..109,112..113,
                     115..117,119..121,123..125)
                     /site_type="other"
                     /note="prefoldin/TRiC interface [polypeptide binding]"
                     /db_xref="CDD:467481"
     Region          23..64
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:467481"
     Site            order(60..61,65,69,71..80,86,90)
                     /site_type="other"
                     /note="prefoldin oligomer interface [polypeptide binding]"
                     /db_xref="CDD:467481"
     Region          84..125
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:467481"
     CDS             1..137
                     /gene="Pfdn4"
                     /coded_by="XM_077646732.1:72..485"
                     /db_xref="GeneID:144113578"
ORIGIN      
        1 masslrrqpv speegdvqvt skdqeminnf arqnarleda teemkqleke lrnledaade
       61 iliveddipi pyqlgevfvn ldseeaqqml eqakaqnker tavleeetrt ikgimaelks
      121 qlynkfgnni nlepdds
//
LOCUS       XP_077502859             133 aa            linear   INV 25-NOV-2025
DEFINITION  prefoldin subunit 4 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502859
VERSION     XP_077502859.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646733.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="prefoldin subunit 4 isoform X2"
                     /calculated_mol_wt=15111
     Region          19..121
                     /region_name="Prefoldin_4"
                     /note="prefoldin subunit 4; cd23165"
                     /db_xref="CDD:467481"
     Site            order(19,21..24,27..28,31,98,101..102,104..105,108..109,
                     111..113,115..117,119..121)
                     /site_type="other"
                     /note="prefoldin/TRiC interface [polypeptide binding]"
                     /db_xref="CDD:467481"
     Region          19..60
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:467481"
     Site            order(56..57,61,65,67..76,82,86)
                     /site_type="other"
                     /note="prefoldin oligomer interface [polypeptide binding]"
                     /db_xref="CDD:467481"
     Region          80..121
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:467481"
     CDS             1..133
                     /gene="Pfdn4"
                     /coded_by="XM_077646733.1:113..514"
                     /db_xref="GeneID:144113578"
ORIGIN      
        1 masslrrqpe gdvqvtskdq eminnfarqn arledateem kqlekelrnl edaadeiliv
       61 eddipipyql gevfvnldse eaqqmleqak aqnkertavl eeetrtikgi maelksqlyn
      121 kfgnninlep dds
//
LOCUS       XP_077502860             134 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial ribosomal protein S17 [Amblyomma americanum].
ACCESSION   XP_077502860
VERSION     XP_077502860.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646734.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..134
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..134
                     /product="mitochondrial ribosomal protein S17"
                     /calculated_mol_wt=15066
     Region          10..81
                     /region_name="Ribosomal_uS17"
                     /note="Ribosomal protein uS17; cd00364"
                     /db_xref="CDD:466775"
     CDS             1..134
                     /gene="mRpS17"
                     /coded_by="XM_077646734.1:117..521"
                     /db_xref="GeneID:144113579"
ORIGIN      
        1 masaaspalf lgkcvtsamk ktvrvvvtrf tlnkfllahy kerteyevld aneecepgdw
       61 vlikelperl slkiahkvek ivfkngniid pitgqkcift yfvkdvdres evfgltppyk
      121 afpaapemkk isek
//
LOCUS       XP_077502861             284 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin polymerization-promoting protein ringmaker [Amblyomma
            americanum].
ACCESSION   XP_077502861
VERSION     XP_077502861.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646735.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..284
                     /product="tubulin polymerization-promoting protein
                     ringmaker"
                     /calculated_mol_wt=31525
     Region          130..275
                     /region_name="p25-alpha"
                     /note="pfam05517"
                     /db_xref="CDD:461670"
     CDS             1..284
                     /gene="ringer"
                     /coded_by="XM_077646735.1:61..915"
                     /db_xref="GeneID:144113580"
ORIGIN      
        1 msklrksdav agrrdsrrmq vlmvedfdvp ppsaeddscr rrdngasdsd vencsdvade
       61 rpcrssiddd vhwplgvlqe qfqilqeriv devrrvmree fyemrmsmat ppespapgsp
      121 vpptsfdgqf kafakfgdsk stgdaitlsn sdkwfkqakv idgkkitttd tgiyfkqvak
      181 tkkalnlkey qqflevigkn kkveveeikd klstcgppat trttpvatgg avarltdhtk
      241 ytgthkqrfd eagkgrgkeg rqdvaadsgy vsgykdegny nkth
//
LOCUS       XP_077502862             238 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113581 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502862
VERSION     XP_077502862.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646736.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..238
                     /product="uncharacterized protein LOC144113581 isoform X1"
                     /calculated_mol_wt=27775
     CDS             1..238
                     /gene="LOC144113581"
                     /coded_by="XM_077646736.1:22..738"
                     /db_xref="GeneID:144113581"
ORIGIN      
        1 msrtlpkipt nkerqkcshi fhdflvprll gyyfekdikw sspkdgtfrl riepttlslm
       61 drdifqdwie vnslqnkedp mtkiqaatsl hqelnkepci emkhrtmayq yfqitnpklk
      121 syisskteak vvpecwqchm rtnntsttna cecwlnknat pldlprdife eeestnisda
      181 ynifctqdmr isladpeiiw ghplhitgqg fcilrrsviy pyetcwytld gngdlhlt
//
LOCUS       XP_077502863             238 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113581 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502863
VERSION     XP_077502863.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646737.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..238
                     /product="uncharacterized protein LOC144113581 isoform X1"
                     /calculated_mol_wt=27775
     CDS             1..238
                     /gene="LOC144113581"
                     /coded_by="XM_077646737.1:22..738"
                     /db_xref="GeneID:144113581"
ORIGIN      
        1 msrtlpkipt nkerqkcshi fhdflvprll gyyfekdikw sspkdgtfrl riepttlslm
       61 drdifqdwie vnslqnkedp mtkiqaatsl hqelnkepci emkhrtmayq yfqitnpklk
      121 syisskteak vvpecwqchm rtnntsttna cecwlnknat pldlprdife eeestnisda
      181 ynifctqdmr isladpeiiw ghplhitgqg fcilrrsviy pyetcwytld gngdlhlt
//
LOCUS       XP_077502864             207 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113581 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502864
VERSION     XP_077502864.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646738.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..207
                     /product="uncharacterized protein LOC144113581 isoform X2"
                     /calculated_mol_wt=24261
     CDS             1..207
                     /gene="LOC144113581"
                     /coded_by="XM_077646738.1:22..645"
                     /db_xref="GeneID:144113581"
ORIGIN      
        1 msrtlpkipt nkerqkcshi fhdflvprll gyyfekdikw sspkdgtfrl riepttlslm
       61 drdifqdwie vnslqnkedp mtkiqaatsl hqelnkepci emkhrtmayq yfqitnpklk
      121 syisskteak vvpecwqchm rtnntsttna cecwlnknat pldlprdife eeestnisda
      181 ynifctqiyp yetcwytldg ngdlhlt
//
LOCUS       XP_077502865             207 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113581 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502865
VERSION     XP_077502865.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646739.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..207
                     /product="uncharacterized protein LOC144113581 isoform X2"
                     /calculated_mol_wt=24261
     CDS             1..207
                     /gene="LOC144113581"
                     /coded_by="XM_077646739.1:22..645"
                     /db_xref="GeneID:144113581"
ORIGIN      
        1 msrtlpkipt nkerqkcshi fhdflvprll gyyfekdikw sspkdgtfrl riepttlslm
       61 drdifqdwie vnslqnkedp mtkiqaatsl hqelnkepci emkhrtmayq yfqitnpklk
      121 syisskteak vvpecwqchm rtnntsttna cecwlnknat pldlprdife eeestnisda
      181 ynifctqiyp yetcwytldg ngdlhlt
//
LOCUS       XP_077502866             507 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113582 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502866
VERSION     XP_077502866.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646740.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..507
                     /product="uncharacterized protein LOC144113582 isoform X1"
                     /calculated_mol_wt=57655
     CDS             1..507
                     /gene="LOC144113582"
                     /coded_by="XM_077646740.1:124..1647"
                     /db_xref="GeneID:144113582"
ORIGIN      
        1 myhsaehvst isgrygkddv evhtsrsfpg tsndlnasrg fspprkrshe nisstdssnv
       61 hvqisdiagd edivattyfl kickstilkp ysiilqliaw rkfrkdnptr hhlswrfmnv
      121 mypalilmll lfyaalqgla cpgrlefktm katslrnatv ivpqnttpvt sewdyydett
      181 tqkyerpelg elplchhrlt lyilpsvlqv maflygsymf rligvdeely aimekvflqs
      241 sayfteriii titrryllag ivwtlmqvav qvlynsavet eeqlverafs alaewktivl
      301 itlrilgqla mtvvemavll nyctqcemii iylrgvalrl rekritmreg mhellasndy
      361 ishlnrnlgk vtalfiinfs lhaliglflf llnndkrpvv layraaypvv wifamfaply
      421 qasrvtavgl kiskisiesr vfgyqdvqef dldsfvlfvg nmklrakmfg taiwpstllf
      481 vfggtalvay llvicdvltn pvmgsfl
//
LOCUS       XP_077502867             504 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113582 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502867
VERSION     XP_077502867.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646741.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..504
                     /product="uncharacterized protein LOC144113582 isoform X2"
                     /calculated_mol_wt=57285
     CDS             1..504
                     /gene="LOC144113582"
                     /coded_by="XM_077646741.1:58..1572"
                     /db_xref="GeneID:144113582"
ORIGIN      
        1 mdkncgtagt ytgqhwnqvh tsrsfpgtsn dlnasrgfsp prkrshenis stdssnvhvq
       61 isdiagdedi vattyflkic kstilkpysi ilqliawrkf rkdnptrhhl swrfmnvmyp
      121 alilmlllfy aalqglacpg rlefktmkat slrnatvivp qnttpvtsew dyydetttqk
      181 yerpelgelp lchhrltlyi lpsvlqvmaf lygsymfrli gvdeelyaim ekvflqssay
      241 fteriiitit rryllagivw tlmqvavqvl ynsaveteeq lverafsala ewktivlitl
      301 rilgqlamtv vemavllnyc tqcemiiiyl rgvalrlrek ritmregmhe llasndyish
      361 lnrnlgkvta lfiinfslha liglflflln ndkrpvvlay raaypvvwif amfaplyqas
      421 rvtavglkis kisiesrvfg yqdvqefdld sfvlfvgnmk lrakmfgtai wpstllfvfg
      481 gtalvayllv icdvltnpvm gsfl
//
LOCUS       XP_077502868             340 aa            linear   INV 25-NOV-2025
DEFINITION  GDP-fucose transporter nac [Amblyomma americanum].
ACCESSION   XP_077502868
VERSION     XP_077502868.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646742.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..340
                     /product="GDP-fucose transporter nac"
                     /calculated_mol_wt=37586
     Region          12..309
                     /region_name="TPT"
                     /note="Triose-phosphate Transporter family; cl26744"
                     /db_xref="CDD:474852"
     CDS             1..340
                     /gene="nac"
                     /coded_by="XM_077646742.1:220..1242"
                     /db_xref="GeneID:144113584"
ORIGIN      
        1 mgdraivmky friagvvsay wlisislvfi nntllsdkkr kldaplfitf fqcfvsyvlc
       61 iilsflsekl pclfqfpkvs hdfstlkalm plsgffvamv tfnnlclkhv gvafytvsrs
      121 lttvfnvmft ylvlrqttsv pavtccgviv agfllgvnqe gqmgslsvfg vtcgvlasat
      181 lsmysiytkk mlplvgdsvs lltfynnvna vilffplvil fgevpviynf pfltdpvfws
      241 lmlvsgvfgf migyvtmlqi hvtsplthnv sgtakactqt vlavvwfsel ksvlwwvsng
      301 lvllgsaayt rvrqlemvkh hihttkqedk adtkgpvikv
//
LOCUS       XP_077502869             177 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113585 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502869
VERSION     XP_077502869.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646743.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..177
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..177
                     /product="uncharacterized protein LOC144113585 isoform X1"
                     /calculated_mol_wt=19585
     CDS             1..177
                     /gene="LOC144113585"
                     /coded_by="XM_077646743.1:28..561"
                     /db_xref="GeneID:144113585"
ORIGIN      
        1 mrrkahadrs rsrasarrrt rmlapaegtv segrgamarp vrmlscssll vatgvvllaa
       61 agiavachdl eyfrtkqece hqfereirrs ydehhdpars revaccalmr anqcitnlhn
      121 akpcedvshe tlahfsrktq kwncenfdyt pcgsgasits asilllvall afvvnrs
//
LOCUS       XP_077502870             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113585 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502870
VERSION     XP_077502870.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646744.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144113585 isoform X2"
                     /calculated_mol_wt=15594
     CDS             1..141
                     /gene="LOC144113585"
                     /coded_by="XM_077646744.1:182..607"
                     /db_xref="GeneID:144113585"
ORIGIN      
        1 marpvrmlsc ssllvatgvv llaaagiava chdleyfrtk qecehqfere irrsydehhd
       61 parsrevacc almranqcit nlhnakpced vshetlahfs rktqkwncen fdytpcgsga
      121 sitsasilll vallafvvnr s
//
LOCUS       XP_077502871             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113585 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502871
VERSION     XP_077502871.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646745.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144113585 isoform X2"
                     /calculated_mol_wt=15594
     CDS             1..141
                     /gene="LOC144113585"
                     /coded_by="XM_077646745.1:223..648"
                     /db_xref="GeneID:144113585"
ORIGIN      
        1 marpvrmlsc ssllvatgvv llaaagiava chdleyfrtk qecehqfere irrsydehhd
       61 parsrevacc almranqcit nlhnakpced vshetlahfs rktqkwncen fdytpcgsga
      121 sitsasilll vallafvvnr s
//
LOCUS       XP_077502872             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113585 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502872
VERSION     XP_077502872.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646746.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144113585 isoform X2"
                     /calculated_mol_wt=15594
     CDS             1..141
                     /gene="LOC144113585"
                     /coded_by="XM_077646746.1:281..706"
                     /db_xref="GeneID:144113585"
ORIGIN      
        1 marpvrmlsc ssllvatgvv llaaagiava chdleyfrtk qecehqfere irrsydehhd
       61 parsrevacc almranqcit nlhnakpced vshetlahfs rktqkwncen fdytpcgsga
      121 sitsasilll vallafvvnr s
//
LOCUS       XP_077502873             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113585 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502873
VERSION     XP_077502873.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646747.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144113585 isoform X2"
                     /calculated_mol_wt=15594
     CDS             1..141
                     /gene="LOC144113585"
                     /coded_by="XM_077646747.1:152..577"
                     /db_xref="GeneID:144113585"
ORIGIN      
        1 marpvrmlsc ssllvatgvv llaaagiava chdleyfrtk qecehqfere irrsydehhd
       61 parsrevacc almranqcit nlhnakpced vshetlahfs rktqkwncen fdytpcgsga
      121 sitsasilll vallafvvnr s
//
LOCUS       XP_077502874             110 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113586 [Amblyomma americanum].
ACCESSION   XP_077502874
VERSION     XP_077502874.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646748.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..110
                     /product="uncharacterized protein LOC144113586"
                     /calculated_mol_wt=11948
     CDS             1..110
                     /gene="LOC144113586"
                     /coded_by="XM_077646748.1:268..600"
                     /db_xref="GeneID:144113586"
ORIGIN      
        1 mssstyayyq eeprtpkryf nlgvlscclg gllfsagiil vvlgsspqep ealwiagivl
       61 llaggllffl gigsvglyla redrrkreaa rartrnytss lrssdmvlve
//
LOCUS       XP_077502875            1663 aa            linear   INV 25-NOV-2025
DEFINITION  amyotrophic lateral sclerosis 2 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502875
VERSION     XP_077502875.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646749.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1663
                     /product="amyotrophic lateral sclerosis 2 isoform X1"
                     /calculated_mol_wt=183967
     Region          <30..219
                     /region_name="ATS1"
                     /note="Alpha-tubulin suppressor ATS1 and related RCC1
                     domain-containing proteins [Cell cycle control, cell
                     division, chromosome partitioning, Cytoskeleton]; COG5184"
                     /db_xref="CDD:444065"
     Region          <523..637
                     /region_name="ATS1"
                     /note="Alpha-tubulin suppressor ATS1 and related RCC1
                     domain-containing proteins [Cell cycle control, cell
                     division, chromosome partitioning, Cytoskeleton]; COG5184"
                     /db_xref="CDD:444065"
     Region          912..1013
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          <1046..1253
                     /region_name="COG4642"
                     /note="Uncharacterized conserved protein [Function
                     unknown]"
                     /db_xref="CDD:443680"
     Region          1557..1657
                     /region_name="VPS9"
                     /note="Vacuolar sorting protein 9 (VPS9) domain;
                     pfam02204"
                     /db_xref="CDD:460489"
     CDS             1..1663
                     /gene="Als2"
                     /coded_by="XM_077646749.1:230..5221"
                     /db_xref="GeneID:144113587"
ORIGIN      
        1 mgvieetsde dkspevqgil wtcnsplssi sigkytvtsl sfgshhclfl tdngevfafg
       61 knnygqlgsg ntveystvpf hvtgvncaak diacgpnhsa altidglvym wgqnnqgqcg
      121 fpeekllspr kveilkelgk cqecgctvtg gvfvisiscg pvhtlaltgn hevfawgtgp
      181 qlglnaatks lpkridflke kkvlsvacga thslavteni ppaiteddav icsscvvkss
      241 tcplglpcmv skltsaqdda iagnsegsmg nedviessat lkaspdgsee qspsadlqaq
      301 dlhseagslh psdqiqaslv salpvntdvt tsvqdeafpv sgasatkenr hempasevqe
      361 adcdefegve lrrgpmqrlr stsmpskrts silnpehame fldrqlghkp qsktrksvpe
      421 dsisepnkei ssstssvamk nmfsrvtslf vdrlnfvsfa pqvtatgsrs gsaesfelve
      481 sdqipnicns ektenhferr qnssrssdir efqlpkhsmi tqvwswgkgs ygqlghgddl
      541 drmqpclmkh lndsgvlkvs aghghslalt csfsvfswgl ndvsqlghnp aakcistpkl
      601 vamprndlvf diaagqshsl fladcggsyp vmyccgqhqc esspygcqks kkvfpvaalk
      661 kgglitgvfa ggpssgclin lketdevqal yefgaserrc lnslcmvqnk vfshlvkdgs
      721 livdipdyac kplksvistt dmlvsllsan aydltlllqs rhisdalsil ekkdawidvf
      781 wsytravtnf lavggfasls rmlvakigeq esplfspnal shfakdiigd tsqlsshtil
      841 qqlfslplkr igeytrlfsr llasldqhtk lwcslqscln dwlalydist kelklaestk
      901 lfwdsvsqkl adiyrvpecr mlrdskscpl qlvsagrfat hhfvlfhdkf vhthfggfqn
      961 yslelawvea dmksetvfai ttpeemlafs cptpsektew vcclnqairn linsekrenp
     1021 vqsmksghld krltppmirh asyvftklpa ykdatykgsw lkgqlhgfgv ltwpdgrkyt
     1081 grfknnlqhg egeyiapgng gdtvfrgtwk ngklhglgsa rypngnlyeg ffkdglrdgh
     1141 gilkegrfls sssiyigqwl qnkrhgygvl dnekegekym gmwqddcrhg ngimvtlddi
     1201 yyegnfvnnn lsgygtmmfq dntifkgelg aggslngkgv lslsngdtiq gtfcgvwseg
     1261 ikisgtyqka vadeqsisdv dslihrdntg spslsvpafe kwrdifdhcr dmlgihgisd
     1321 tqdnkhawda iaiaisnrkr qshvkkpakp cceenldyle riprtsrkhg sltlehytei
     1381 keylssafdc vhhplgylld glvgvfrlty cgigvhprll vhaveevrsf chrlyelvri
     1441 lfpdlphssk pmvlttlgdi espddffdvt gecitpntli qpvllprlyp slstlyalnn
     1501 eqedrhywer llkwnkqsdl almtflgvde kfmslkfpdk iftmernrvs sikdecftna
     1561 idalqhista ftafdklqai rqtfeeinka iqkglgdflw nmddlfpvfq yvvvrarirn
     1621 lgseihfvdd lmerhfqnge lgimfttvka cyfqirtekl iqa
//
LOCUS       XP_077502876            1663 aa            linear   INV 25-NOV-2025
DEFINITION  amyotrophic lateral sclerosis 2 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502876
VERSION     XP_077502876.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646750.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1663
                     /product="amyotrophic lateral sclerosis 2 isoform X2"
                     /calculated_mol_wt=184011
     Region          <30..219
                     /region_name="ATS1"
                     /note="Alpha-tubulin suppressor ATS1 and related RCC1
                     domain-containing proteins [Cell cycle control, cell
                     division, chromosome partitioning, Cytoskeleton]; COG5184"
                     /db_xref="CDD:444065"
     Region          <523..637
                     /region_name="ATS1"
                     /note="Alpha-tubulin suppressor ATS1 and related RCC1
                     domain-containing proteins [Cell cycle control, cell
                     division, chromosome partitioning, Cytoskeleton]; COG5184"
                     /db_xref="CDD:444065"
     Region          912..1013
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          <1046..1253
                     /region_name="COG4642"
                     /note="Uncharacterized conserved protein [Function
                     unknown]"
                     /db_xref="CDD:443680"
     Region          1557..1657
                     /region_name="VPS9"
                     /note="Vacuolar sorting protein 9 (VPS9) domain;
                     pfam02204"
                     /db_xref="CDD:460489"
     CDS             1..1663
                     /gene="Als2"
                     /coded_by="XM_077646750.1:230..5221"
                     /db_xref="GeneID:144113587"
ORIGIN      
        1 mgvieetsde dkspevqgil wtcnsplssi sigkytvtsl sfgshhclfl tdngevfafg
       61 knnygqlgsg ntveystvpf hvtgvncaak diacgpnhsa altidglvym wgqnnqgqcg
      121 fpeekllspr kveilkelgk cqecgctvtg gvfvisiscg pvhtlaltgn hevfawgtgp
      181 qlglnaatks lpkridflke kkvlsvacga thslavteni ppaiteddav icsscvvkss
      241 tcplglpcmv skltsaqdda iagnsegsmg nedviessat lkaspdgsee qspsadlqaq
      301 dlhseagslh psdqiqaslv salpvntdvt tsvqdeafpv sgasatkenr hempasevqe
      361 adcdefegve lrrgpmqrlr stsmpskrts silnpehame fldrqlghkp qsktrksvpe
      421 dsisepnkei ssstssvamk nmfsrvtslf vdrlnfvsfa pqvtatgsrs gsaesfelve
      481 sdqipnicns ektenhferr qnssrssdir efqlpkhsmi tqvwswgkgs ygqlghgddl
      541 drmqpclmkh lndsgvlkvs aghghslalt csfsvfswgl ndvsqlghnp aakcistpkl
      601 vamprndlvf diaagqshsl fladcggsyp vmyccgqhqc esspygcqks kkvfpvaalk
      661 kgglitgvfa ggpssgclin lketdevqal yefgaserrc lnslcmvqnk vfshlvkdgs
      721 livdipdyac kplksvistt dmlvsllsan aydltlllqs rhisdalsil ekkdawidvf
      781 wsytravtnf lavggfasls rmlvakigeq esplfspnal shfakdiigd tsqlsshtil
      841 qqlfslplkr igeytrlfsr llasldqhtk lwcslqscln dwlalydist kelklaestk
      901 lfwdsvsqkl adiyrvpecr mlrdskscpl qlvsagrfat hhfvlfhdkf vhthfggfqn
      961 yslelawvea dmksetvfai ttpeemlafs cptpsektew vcclnqairn linsekrenp
     1021 vqsmksghld krltppmirh asyvftklpa ykdatykgsw lkgqlhgfgv ltwpdgrkyt
     1081 grfknnlqhg egeyiapgng gdtvfrgtwk ngklhglgsa rypngnlyeg ffkdglrdgh
     1141 gilkegrfls sssiyigqwl qnkrhgygvl dnekegekym gmwqddcrhg ngimvtlddi
     1201 yyegnfvnnn lsgygtmmfq dntifkgelg aggslngkgv lslsngdtiq gtfcgvwseg
     1261 ikisgtyqka vadeqsisdv dslihrdntg spslsvpafe kwrdifdhcr dmlgihgisd
     1321 tqdnkhawda iaiaisnrkr qshvkkpakp cceenldyle riprtsrkhg sltlehytei
     1381 keylssafdc vhhplgylld glvgvfrlty cgigvhprll vhaveevrsf chrlyelvri
     1441 lfpdlphssk pmvlttlgdi espddffdvt gecitpntli qpvllprlyp slstlyalnn
     1501 eqedrhywer llkwnkqsdl almtflgvde kfmslkfpdk iftmernrvs sikdecftna
     1561 idalqhista ftafdklqai rqtfeeinke vqkglgdflw nmddlfpvfq yvvvrarirn
     1621 lgseihfvdd lmerhfqnge lgimfttvka cyfqirtekl iqa
//
LOCUS       XP_077502877            1653 aa            linear   INV 25-NOV-2025
DEFINITION  amyotrophic lateral sclerosis 2 isoform X3 [Amblyomma americanum].
ACCESSION   XP_077502877
VERSION     XP_077502877.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646751.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1653
                     /product="amyotrophic lateral sclerosis 2 isoform X3"
                     /calculated_mol_wt=182913
     Region          <30..219
                     /region_name="ATS1"
                     /note="Alpha-tubulin suppressor ATS1 and related RCC1
                     domain-containing proteins [Cell cycle control, cell
                     division, chromosome partitioning, Cytoskeleton]; COG5184"
                     /db_xref="CDD:444065"
     Region          <523..637
                     /region_name="ATS1"
                     /note="Alpha-tubulin suppressor ATS1 and related RCC1
                     domain-containing proteins [Cell cycle control, cell
                     division, chromosome partitioning, Cytoskeleton]; COG5184"
                     /db_xref="CDD:444065"
     Region          902..1003
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          <1036..1243
                     /region_name="COG4642"
                     /note="Uncharacterized conserved protein [Function
                     unknown]"
                     /db_xref="CDD:443680"
     Region          1547..1647
                     /region_name="VPS9"
                     /note="Vacuolar sorting protein 9 (VPS9) domain;
                     pfam02204"
                     /db_xref="CDD:460489"
     CDS             1..1653
                     /gene="Als2"
                     /coded_by="XM_077646751.1:217..5178"
                     /db_xref="GeneID:144113587"
ORIGIN      
        1 mgvieetsde dkspevqgil wtcnsplssi sigkytvtsl sfgshhclfl tdngevfafg
       61 knnygqlgsg ntveystvpf hvtgvncaak diacgpnhsa altidglvym wgqnnqgqcg
      121 fpeekllspr kveilkelgk cqecgctvtg gvfvisiscg pvhtlaltgn hevfawgtgp
      181 qlglnaatks lpkridflke kkvlsvacga thslavteni ppaiteddav icsscvvkss
      241 tcplglpcmv skltsaqdda iagnsegsmg nedviessat lkaspdgsee qspsadlqaq
      301 dlhseagslh psdqiqaslv salpvntdvt tsvqdeafpv sgasatkenr hempasevqe
      361 adcdefegve lrrgpmqrlr stsmpskrts silnpehame fldrqlghkp qsktrksvpe
      421 dsisepnkei ssstssvamk nmfsrvtslf vdrlnfvsfa pqvtatgsrs gsaesfelve
      481 sdqipnicns ektenhferr qnssrssdir efqlpkhsmi tqvwswgkgs ygqlghgddl
      541 drmqpclmkh lndsgvlkvs aghghslalt csfsvfswgl ndvsqlghnp aakcistpkl
      601 vamprndlvf diaagqshsl fladcggsyp vmyccgqhqc esspygcqks kkvfpvaalk
      661 kgglitgvfa ggpssgclin lketdevqal yefgaserrc lnslcmvqnk vfshlvkdgs
      721 livdipdyac kplksvistt dmlvsllsan aydltlllqs rhisdalsil ekkdawidvf
      781 wsytravtnf lavggfasls rmlvfspnal shfakdiigd tsqlsshtil qqlfslplkr
      841 igeytrlfsr llasldqhtk lwcslqscln dwlalydist kelklaestk lfwdsvsqkl
      901 adiyrvpecr mlrdskscpl qlvsagrfat hhfvlfhdkf vhthfggfqn yslelawvea
      961 dmksetvfai ttpeemlafs cptpsektew vcclnqairn linsekrenp vqsmksghld
     1021 krltppmirh asyvftklpa ykdatykgsw lkgqlhgfgv ltwpdgrkyt grfknnlqhg
     1081 egeyiapgng gdtvfrgtwk ngklhglgsa rypngnlyeg ffkdglrdgh gilkegrfls
     1141 sssiyigqwl qnkrhgygvl dnekegekym gmwqddcrhg ngimvtlddi yyegnfvnnn
     1201 lsgygtmmfq dntifkgelg aggslngkgv lslsngdtiq gtfcgvwseg ikisgtyqka
     1261 vadeqsisdv dslihrdntg spslsvpafe kwrdifdhcr dmlgihgisd tqdnkhawda
     1321 iaiaisnrkr qshvkkpakp cceenldyle riprtsrkhg sltlehytei keylssafdc
     1381 vhhplgylld glvgvfrlty cgigvhprll vhaveevrsf chrlyelvri lfpdlphssk
     1441 pmvlttlgdi espddffdvt gecitpntli qpvllprlyp slstlyalnn eqedrhywer
     1501 llkwnkqsdl almtflgvde kfmslkfpdk iftmernrvs sikdecftna idalqhista
     1561 ftafdklqai rqtfeeinka iqkglgdflw nmddlfpvfq yvvvrarirn lgseihfvdd
     1621 lmerhfqnge lgimfttvka cyfqirtekl iqa
//
LOCUS       XP_077502878            1653 aa            linear   INV 25-NOV-2025
DEFINITION  amyotrophic lateral sclerosis 2 isoform X4 [Amblyomma americanum].
ACCESSION   XP_077502878
VERSION     XP_077502878.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646752.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1653
                     /product="amyotrophic lateral sclerosis 2 isoform X4"
                     /calculated_mol_wt=182957
     Region          <30..219
                     /region_name="ATS1"
                     /note="Alpha-tubulin suppressor ATS1 and related RCC1
                     domain-containing proteins [Cell cycle control, cell
                     division, chromosome partitioning, Cytoskeleton]; COG5184"
                     /db_xref="CDD:444065"
     Region          <523..637
                     /region_name="ATS1"
                     /note="Alpha-tubulin suppressor ATS1 and related RCC1
                     domain-containing proteins [Cell cycle control, cell
                     division, chromosome partitioning, Cytoskeleton]; COG5184"
                     /db_xref="CDD:444065"
     Region          902..1003
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          <1036..1243
                     /region_name="COG4642"
                     /note="Uncharacterized conserved protein [Function
                     unknown]"
                     /db_xref="CDD:443680"
     Region          1547..1647
                     /region_name="VPS9"
                     /note="Vacuolar sorting protein 9 (VPS9) domain;
                     pfam02204"
                     /db_xref="CDD:460489"
     CDS             1..1653
                     /gene="Als2"
                     /coded_by="XM_077646752.1:230..5191"
                     /db_xref="GeneID:144113587"
ORIGIN      
        1 mgvieetsde dkspevqgil wtcnsplssi sigkytvtsl sfgshhclfl tdngevfafg
       61 knnygqlgsg ntveystvpf hvtgvncaak diacgpnhsa altidglvym wgqnnqgqcg
      121 fpeekllspr kveilkelgk cqecgctvtg gvfvisiscg pvhtlaltgn hevfawgtgp
      181 qlglnaatks lpkridflke kkvlsvacga thslavteni ppaiteddav icsscvvkss
      241 tcplglpcmv skltsaqdda iagnsegsmg nedviessat lkaspdgsee qspsadlqaq
      301 dlhseagslh psdqiqaslv salpvntdvt tsvqdeafpv sgasatkenr hempasevqe
      361 adcdefegve lrrgpmqrlr stsmpskrts silnpehame fldrqlghkp qsktrksvpe
      421 dsisepnkei ssstssvamk nmfsrvtslf vdrlnfvsfa pqvtatgsrs gsaesfelve
      481 sdqipnicns ektenhferr qnssrssdir efqlpkhsmi tqvwswgkgs ygqlghgddl
      541 drmqpclmkh lndsgvlkvs aghghslalt csfsvfswgl ndvsqlghnp aakcistpkl
      601 vamprndlvf diaagqshsl fladcggsyp vmyccgqhqc esspygcqks kkvfpvaalk
      661 kgglitgvfa ggpssgclin lketdevqal yefgaserrc lnslcmvqnk vfshlvkdgs
      721 livdipdyac kplksvistt dmlvsllsan aydltlllqs rhisdalsil ekkdawidvf
      781 wsytravtnf lavggfasls rmlvfspnal shfakdiigd tsqlsshtil qqlfslplkr
      841 igeytrlfsr llasldqhtk lwcslqscln dwlalydist kelklaestk lfwdsvsqkl
      901 adiyrvpecr mlrdskscpl qlvsagrfat hhfvlfhdkf vhthfggfqn yslelawvea
      961 dmksetvfai ttpeemlafs cptpsektew vcclnqairn linsekrenp vqsmksghld
     1021 krltppmirh asyvftklpa ykdatykgsw lkgqlhgfgv ltwpdgrkyt grfknnlqhg
     1081 egeyiapgng gdtvfrgtwk ngklhglgsa rypngnlyeg ffkdglrdgh gilkegrfls
     1141 sssiyigqwl qnkrhgygvl dnekegekym gmwqddcrhg ngimvtlddi yyegnfvnnn
     1201 lsgygtmmfq dntifkgelg aggslngkgv lslsngdtiq gtfcgvwseg ikisgtyqka
     1261 vadeqsisdv dslihrdntg spslsvpafe kwrdifdhcr dmlgihgisd tqdnkhawda
     1321 iaiaisnrkr qshvkkpakp cceenldyle riprtsrkhg sltlehytei keylssafdc
     1381 vhhplgylld glvgvfrlty cgigvhprll vhaveevrsf chrlyelvri lfpdlphssk
     1441 pmvlttlgdi espddffdvt gecitpntli qpvllprlyp slstlyalnn eqedrhywer
     1501 llkwnkqsdl almtflgvde kfmslkfpdk iftmernrvs sikdecftna idalqhista
     1561 ftafdklqai rqtfeeinke vqkglgdflw nmddlfpvfq yvvvrarirn lgseihfvdd
     1621 lmerhfqnge lgimfttvka cyfqirtekl iqa
//
LOCUS       XP_077502879            1157 aa            linear   INV 25-NOV-2025
DEFINITION  low-density lipoprotein receptor-related protein 2-like [Amblyomma
            americanum].
ACCESSION   XP_077502879
VERSION     XP_077502879.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646753.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1157
                     /product="low-density lipoprotein receptor-related protein
                     2-like"
                     /calculated_mol_wt=128390
     Region          38..71
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     Region          148..190
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          193..234
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          249..283
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          357..392
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     Region          822..864
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          865..907
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          1001..1029
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     CDS             1..1157
                     /gene="LOC144113588"
                     /coded_by="XM_077646753.1:236..3709"
                     /db_xref="GeneID:144113588"
ORIGIN      
        1 mlsaclfvhl lpslfafafs iaeslqagng eslcnewgrc aqtcvqdwrn rslivcgcve
       61 gfrldsnrvs clpldplasy llittnagiq qsfveskgks qflynsntla vavdflfngs
      121 ktivfwsdin tdqinagevv agrlenvrsv vmgglesveg lavdwvtcnl ywvesrhqei
      181 evsrcdgsmr asvvgdvrhp rglaldprvg flfwtdwddk npriersyld gssrkvivkv
      241 eeysnlpksl pngitldyav hrvywidags gsihtvkydg sghqsilhls kfakpfsmdv
      301 fgdyfywtdi tfssifkahr fslnasqevv lrsnnslyqa aivhpsrhpt ynatnycayk
      361 ngfcshlcvl gngqplcmcp yglqlgldkr tceahgplii yatkdanvvy falasdpsqw
      421 iyppmklhaa afaltydsrn evlywiegra ikkhvlktyt teifaefgsl vkltalsfdc
      481 lsgnlyvgns vqlnseirsq isvcatggry csnliwdsvr hvsglavdai nrlifwmere
      541 raaadqdlmt vkaahlngsv swtlhtwnsp knvpvkgldv nplqeevcws dankgalmca
      601 syqttgnfse vllsgggsqg hvvfaasgkk llyslgngsi ysmthssrdi phlvwnstqp
      661 lsymklasad dfpehlvsrh pcqqlpvpcn giclpqegsh tclplggsdt llvflqnhrt
      721 vvypvgsdpt vpplllqaqf sempldsihv lpteeavifs srerlyrari dgydvtpmav
      781 lnntrvtala adassgllyr avyssqgari esfylngsgv svlvdtnlhr vnalvvhpfr
      841 rllfwsdage wprierfdle tkerqtivhd eliqvsdltl dlerdlvywc dlgtariera
      901 rldgtrrtsi vlnkglnrhy spvsvavfqs vlfwidstys ggslvmlvrn rhpqistwsr
      961 ylgpnmshlq lynrhmlsyl nessnaygsn lnpimscrgf cfnggtclav ddspggptcq
     1021 ctsnftgprc qfyaayiynp pfsdeepadv kiivipvavl lsafllmlvv awvkrrrawq
     1081 stavslssee anpalaarmi dnqyetsfsd nvllmatnla fedmepapgn sntlnglesl
     1141 prkplktamm gswlqsv
//
LOCUS       XP_077502880             457 aa            linear   INV 25-NOV-2025
DEFINITION  dihydrouridine synthase 2 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502880
VERSION     XP_077502880.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646754.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..457
                     /product="dihydrouridine synthase 2 isoform X1"
                     /calculated_mol_wt=51214
     Region          10..255
                     /region_name="DUS_like_FMN"
                     /note="Dihydrouridine synthase-like (DUS-like) FMN-binding
                     domain. Members of this family catalyze the reduction of
                     the 5,6-double bond of a uridine residue on tRNA.
                     Dihydrouridine modification of tRNA is widely observed in
                     prokaryotes and eukaryotes, and also...; cd02801"
                     /db_xref="CDD:239200"
     Site            order(14..16,40,84,110,152,180,211,213,238..239)
                     /site_type="other"
                     /note="FMN binding site [chemical binding]"
                     /db_xref="CDD:239200"
     Site            order(84,113..114,152,154,179..180,182..183,212..213,239)
                     /site_type="active"
                     /db_xref="CDD:239200"
     Site            order(113,154,180,182)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:239200"
     Site            order(114,152,179,183,212..213)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:239200"
     Region          370..>432
                     /region_name="DSRM_SF"
                     /note="double-stranded RNA binding motif (DSRM)
                     superfamily; cl00054"
                     /db_xref="CDD:444671"
     Site            order(376..377,379,398,428..430)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:380679"
     CDS             1..457
                     /gene="Dus2"
                     /coded_by="XM_077646754.1:176..1549"
                     /db_xref="GeneID:144113590"
ORIGIN      
        1 mtssvdyrnk vilapmvrig tlpmrllalh ygadlvytee lidyrllkcq ridnkvlgti
       61 dfvdddhqiv frtcekekgr nilqigtcnp eravqvaklv erdvagidvn mgcpkefsik
      121 ggmgaalltq adkvkailta lvqstdlpvt ckirvldkle etlalgklie stgvkaiavh
      181 grtkeerpqh anrnavikal aehvgipvia nggsgeitcy edidrfrqat gassvmlarq
      241 aenncsifrk egkkpiddvi eqylayaiey dnratntkyc vqqmlgslqe sdrgkallas
      301 qqmeeicvlw nmedkhasrq lklqarakam relsngdnse pvlkkckvgd eevwqmeakf
      361 vrnmfgmanl pktvlinwtr knnyphpvyk tesmeksfrs vvlvnrkrys stylcpqkfs
      421 ergncgkarr fahlsvpalp rvpctvrpsk mgnisyk
//
LOCUS       XP_077502881             451 aa            linear   INV 25-NOV-2025
DEFINITION  dihydrouridine synthase 2 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502881
VERSION     XP_077502881.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646755.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..451
                     /product="dihydrouridine synthase 2 isoform X2"
                     /calculated_mol_wt=50348
     Region          10..255
                     /region_name="DUS_like_FMN"
                     /note="Dihydrouridine synthase-like (DUS-like) FMN-binding
                     domain. Members of this family catalyze the reduction of
                     the 5,6-double bond of a uridine residue on tRNA.
                     Dihydrouridine modification of tRNA is widely observed in
                     prokaryotes and eukaryotes, and also...; cd02801"
                     /db_xref="CDD:239200"
     Site            order(14..16,40,84,110,152,180,211,213,238..239)
                     /site_type="other"
                     /note="FMN binding site [chemical binding]"
                     /db_xref="CDD:239200"
     Site            order(84,113..114,152,154,179..180,182..183,212..213,239)
                     /site_type="active"
                     /db_xref="CDD:239200"
     Site            order(113,154,180,182)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:239200"
     Site            order(114,152,179,183,212..213)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:239200"
     Region          370..435
                     /region_name="DSRM_DUS2L"
                     /note="double-stranded RNA binding motif of
                     tRNA-dihydrouridine(20) synthase [NAD(P)+]-like (DUS2L)
                     and similar proteins; cd19871"
                     /db_xref="CDD:380700"
     Site            order(370,372..373,376..377,380..381,386..387,395..396,
                     398,416..419,422..423)
                     /site_type="other"
                     /note="putative RNA binding site [nucleotide binding]"
                     /db_xref="CDD:380700"
     CDS             1..451
                     /gene="Dus2"
                     /coded_by="XM_077646755.1:176..1531"
                     /db_xref="GeneID:144113590"
ORIGIN      
        1 mtssvdyrnk vilapmvrig tlpmrllalh ygadlvytee lidyrllkcq ridnkvlgti
       61 dfvdddhqiv frtcekekgr nilqigtcnp eravqvaklv erdvagidvn mgcpkefsik
      121 ggmgaalltq adkvkailta lvqstdlpvt ckirvldkle etlalgklie stgvkaiavh
      181 grtkeerpqh anrnavikal aehvgipvia nggsgeitcy edidrfrqat gassvmlarq
      241 aenncsifrk egkkpiddvi eqylayaiey dnratntkyc vqqmlgslqe sdrgkallas
      301 qqmeeicvlw nmedkhasrq lklqarakam relsngdnse pvlkkckvgd eevwqmeakf
      361 vrnmfgmanl pktvlinwtr knnyphpvyk tesmeksfrs vvlvnrkrys styleknkky
      421 aeqatalval yalglidssk ikgntagmpv e
//
LOCUS       XP_077502882             443 aa            linear   INV 25-NOV-2025
DEFINITION  dihydrouridine synthase 2 isoform X3 [Amblyomma americanum].
ACCESSION   XP_077502882
VERSION     XP_077502882.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646756.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="dihydrouridine synthase 2 isoform X3"
                     /calculated_mol_wt=49825
     Region          10..255
                     /region_name="DUS_like_FMN"
                     /note="Dihydrouridine synthase-like (DUS-like) FMN-binding
                     domain. Members of this family catalyze the reduction of
                     the 5,6-double bond of a uridine residue on tRNA.
                     Dihydrouridine modification of tRNA is widely observed in
                     prokaryotes and eukaryotes, and also...; cd02801"
                     /db_xref="CDD:239200"
     Site            order(14..16,40,84,110,152,180,211,213,238..239)
                     /site_type="other"
                     /note="FMN binding site [chemical binding]"
                     /db_xref="CDD:239200"
     Site            order(84,113..114,152,154,179..180,182..183,212..213,239)
                     /site_type="active"
                     /db_xref="CDD:239200"
     Site            order(113,154,180,182)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:239200"
     Site            order(114,152,179,183,212..213)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:239200"
     Region          370..>413
                     /region_name="DSRM_SF"
                     /note="double-stranded RNA binding motif (DSRM)
                     superfamily; cl00054"
                     /db_xref="CDD:444671"
     CDS             1..443
                     /gene="Dus2"
                     /coded_by="XM_077646756.1:176..1507"
                     /db_xref="GeneID:144113590"
ORIGIN      
        1 mtssvdyrnk vilapmvrig tlpmrllalh ygadlvytee lidyrllkcq ridnkvlgti
       61 dfvdddhqiv frtcekekgr nilqigtcnp eravqvaklv erdvagidvn mgcpkefsik
      121 ggmgaalltq adkvkailta lvqstdlpvt ckirvldkle etlalgklie stgvkaiavh
      181 grtkeerpqh anrnavikal aehvgipvia nggsgeitcy edidrfrqat gassvmlarq
      241 aenncsifrk egkkpiddvi eqylayaiey dnratntkyc vqqmlgslqe sdrgkallas
      301 qqmeeicvlw nmedkhasrq lklqarakam relsngdnse pvlkkckvgd eevwqmeakf
      361 vrnmfgmanl pktvlinwtr knnyphpvyk tesmeksfrs vvlvnrkrys styllqqhft
      421 slrelpqwls gygarlltqk tqv
//
LOCUS       XP_077502883             748 aa            linear   INV 25-NOV-2025
DEFINITION  vesicle-fusing ATPase 2-like [Amblyomma americanum].
ACCESSION   XP_077502883
VERSION     XP_077502883.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646757.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..748
                     /product="vesicle-fusing ATPase 2-like"
                     /calculated_mol_wt=82963
     Region          8..86
                     /region_name="CDC48_N"
                     /note="Cell division protein 48 (CDC48) N-terminal domain;
                     smart01073"
                     /db_xref="CDD:215012"
     Region          114..168
                     /region_name="CDC48_2"
                     /note="Cell division protein 48 (CDC48) domain 2;
                     smart01072"
                     /db_xref="CDD:215011"
     Region          225..401
                     /region_name="RecA-like_NSF-SEC18_r1-like"
                     /note="first of two ATPase domains of NSF and SEC18, and
                     similar ATPase domains; cd19504"
                     /db_xref="CDD:410912"
     Region          <226..>612
                     /region_name="CDC48"
                     /note="AAA family ATPase, CDC48 subfamily; TIGR01243"
                     /db_xref="CDD:273521"
     Site            order(227,270..274,334)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:410912"
     Site            order(239,242,246,252..256,301..302,305,309,358..359,362,
                     366..367,392)
                     /site_type="other"
                     /note="hexamer interface [polypeptide binding]"
                     /db_xref="CDD:410912"
     CDS             1..748
                     /gene="LOC144113591"
                     /coded_by="XM_077646757.1:297..2543"
                     /db_xref="GeneID:144113591"
ORIGIN      
        1 mmsatkqmkv drcpteefsl sncaavnpvd fsedkvrhve vtttnhkfvf tiktsdkvrp
       61 gtmgfslpmr kwaelsvsqm vdvrpyvfdp ntqyiakitl eadflekkni tkepydtdkm
      121 stdfvqqfpk qaftvgqila yqfadkkllk lvvkemevas ldylrgdtsk pqpkpktthi
      181 glllpdaavv fekgegstls ltghakgkiv rqsiinpdwd fnkmgiggld kefsaifrra
      241 fasrvfppev ieqldlkhvr gillygppgt gktlmarqig kmlngrepki vngpqilnky
      301 vgeseanirr lfedaeeeek klginsglhi iifdeidaic kqrgsvagnt gvhdtvvnql
      361 lskidgvesl nnilvigmtn rrdmidealt rpgrlevqme iglpdeagrl qilnihtaqm
      421 rnhrkmapdv dlaelallsk nfsgaelegl vraaqstamn rlikagskve ldpeaaekll
      481 vtradfmnal endvkpafgt steeieqlvs kgvttwgpqi sailedgdlf iqqarspesr
      541 glvtillega pnsgktalaa kialrsefpf iklcspdamv gytetakcql ikkvfddayk
      601 sqqscilvdn ierlldygpi gprysnlvlq allvllqkdp pkgrkllvlc tsslkgvlde
      661 mglsssftav lrvpnlsqsq hvlavledvd cfskaeldqi srhlegrriw igikkllayi
      721 dmarqtasqy rvmkfickle eegalelk
//
LOCUS       XP_077502885             155 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113593 [Amblyomma americanum].
ACCESSION   XP_077502885
VERSION     XP_077502885.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646759.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..155
                     /product="uncharacterized protein LOC144113593"
                     /calculated_mol_wt=17828
     CDS             1..155
                     /gene="LOC144113593"
                     /coded_by="XM_077646759.1:647..1114"
                     /db_xref="GeneID:144113593"
ORIGIN      
        1 mtskclvtfr ernfvlpfdg tltrrglver vrdhelfrgi dvatliltmf eedfnvfvdi
       61 pedfeirdre kislrglpgs dcgcsrrapa sgaipchchv qphiqpasct krypiqyrah
      121 qsgqyfkars rvvqwlyhdl clytmypgkl yneaa
//
LOCUS       XP_077502886             559 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113594 [Amblyomma americanum].
ACCESSION   XP_077502886
VERSION     XP_077502886.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646760.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..559
                     /product="uncharacterized protein LOC144113594"
                     /calculated_mol_wt=60885
     Region          19..105
                     /region_name="THAP"
                     /note="The THAP domain is a putative DNA-binding domain
                     (DBD) and probably also binds a zinc ion; smart00980"
                     /db_xref="CDD:214951"
     Region          303..350
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          381..541
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..559
                     /gene="LOC144113594"
                     /coded_by="XM_077646760.1:83..1762"
                     /db_xref="GeneID:144113594"
ORIGIN      
        1 maasgsaect rsksrssiyc cvygchnsyk ntagklpkik fysfpwrpye eerrqrwvra
       61 vrraspdgsp wqpnrcstri csahfvgnek stiaghpayi ptlfpasygr cdgvcsetki
      121 gryeraqrrg ltteastsgk lrtaaeavgs qsevsgadgl saeatapdep ettadasspk
      181 aaasgaggaq sssdsvatml svatqtegsl hqgqctlfls vasqgcastq vchndtvdts
      241 vqaqvatest fagpeerscv flgyesmchk eeafrelcgv sanvfallls vispipvrqi
      301 dvpvaqklai flmrlklgts fssiavlfgl hrtavsrify fvlsnmlaal qnfipnpsra
      361 tvdalmpacf mrhyprcrfi idctevrtee pptleqrral fshykggytf kflvgilpng
      421 citfvldayg grtsdthitl esgfldriep gdvvladkgf pgikapaegk egivvlppfs
      481 kgnvqftcee lqetyhvaqv rihvesviqr lklfnvlntr vavdmiphms simrmccilv
      541 nlqnpvintk wpqvnkngy
//
LOCUS       XP_077502887            1011 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113595 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502887
VERSION     XP_077502887.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646761.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1011
                     /product="uncharacterized protein LOC144113595 isoform X1"
                     /calculated_mol_wt=115096
     Region          121..138
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          142..165
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <158..466
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          166..188
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          211..234
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          235..258
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          259..281
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          282..305
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          306..352
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          353..376
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          377..399
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          398..556
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     Region          400..422
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          423..446
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          447..468
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          471..493
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          494..540
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          540..579
                     /region_name="LRR_4"
                     /note="Leucine Rich repeats (2 copies); pfam12799"
                     /db_xref="CDD:463713"
     Region          541..561
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          566..618
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          574..618
                     /region_name="LRRCT"
                     /note="Leucine rich repeat C-terminal domain; smart00082"
                     /db_xref="CDD:214507"
     Region          611..650
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          619..687
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          626..666
                     /region_name="LRRNT"
                     /note="Leucine rich repeat N-terminal domain; smart00013"
                     /db_xref="CDD:214470"
     Region          688..709
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          859..988
                     /region_name="TIR"
                     /note="Toll - interleukin 1 - resistance; smart00255"
                     /db_xref="CDD:214587"
     CDS             1..1011
                     /gene="LOC144113595"
                     /coded_by="XM_077646761.1:150..3185"
                     /db_xref="GeneID:144113595"
ORIGIN      
        1 mdhwmcsvrq awecsfrcsg rarnmvqggp isasvrhlfg taifagmrta aqltlaglvl
       61 iaalsrrqce aaglgghcpi sldwcacmvv rrgprvlcna tadgdavsgd lsrlagfamd
      121 slildgvnlt tlprryfvnl tvkalvvrnt pmqsteedsf dglthlttld mshnrlrtip
      181 lgltafptlk ilrlpdnyia dfknlpplen lteldlsknl iekldgnvlk dfpklqrvwl
      241 annliiflst sffkgaknvt fvdlhnnmlt niytafqglh hlmevdlsrn siigidklst
      301 gttprlkilr asynsiswls rfgpvniale tielthcnis elhretfsql pellrldlsy
      361 naiqyipgny fhkesklkyf saagnklfsi tntfsvtrrm evlnlsmnfi edledslfnm
      421 palkkltlrn nrikfirdgt frsnsyikhl dlsgndlkwv gidsferlfs levllmtgts
      481 vmslngslhe lpklrhlfih dnrlkklrvv dfanspqivs idakrnnitd vqgafrkldn
      541 lkslslrrnr lksihrasfp nkldrlktlv ldenplfcdc qlswlietap sllwkgvpvc
      601 hgpdwnrgkq lyslsadnlt ewfancsqsc ncvchkntsf saailvncsr rglqalpkef
      661 pwktylldls wnrlrqldnr lvhktpslds llmannllsd igtgviprst ekldltknrl
      721 rrfplglvse rtvasvwlsg npwkcscedy pfrqwaeshs yairdynetl ceqgqnllts
      781 gkkfmdlgek dlcpsqkvfl ilaygvpsmv lvvtamaavt aylkykheir vwlyargicr
      841 slqcikeddv dedkdfdvfl sfsskdrewa ysellpkvea ngfsvctydr nfkggfliqd
      901 ivqeavccsr rtllvvtqny vesewcrwef rvaqhraled ninrlivvvv gdvrpegvde
      961 elqrymqetn ylrwgeahfw dkllyslpkr dartrvipnm hplthmatpa i
//
LOCUS       XP_077502888             965 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113595 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502888
VERSION     XP_077502888.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646762.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..965
                     /product="uncharacterized protein LOC144113595 isoform X2"
                     /calculated_mol_wt=109998
     Region          75..92
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          96..119
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <112..420
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          120..142
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          165..188
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          189..212
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          213..235
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          236..259
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          260..306
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          307..330
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          331..353
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          352..510
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     Region          354..376
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          377..400
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          401..422
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          425..447
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          448..494
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          495..515
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          520..572
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          528..572
                     /region_name="LRRCT"
                     /note="Leucine rich repeat C-terminal domain; smart00082"
                     /db_xref="CDD:214507"
     Region          565..604
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          573..641
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          580..620
                     /region_name="LRRNT"
                     /note="Leucine rich repeat N-terminal domain; smart00013"
                     /db_xref="CDD:214470"
     Region          642..663
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          813..942
                     /region_name="TIR"
                     /note="Toll - interleukin 1 - resistance; smart00255"
                     /db_xref="CDD:214587"
     CDS             1..965
                     /gene="LOC144113595"
                     /coded_by="XM_077646762.1:161..3058"
                     /db_xref="GeneID:144113595"
ORIGIN      
        1 mrtaaqltla glvliaalsr rqceaaglgg hcpisldwca cmvvrrgprv lcnatadgda
       61 vsgdlsrlag famdslildg vnlttlprry fvnltvkalv vrntpmqste edsfdglthl
      121 ttldmshnrl rtiplgltaf ptlkilrlpd nyiadfknlp plenlteldl sknliekldg
      181 nvlkdfpklq rvwlannlii flstsffkga knvtfvdlhn nmltniytaf qglhhlmevd
      241 lsrnsiigid klstgttprl kilrasynsi swlsrfgpvn ialetielth cniselhret
      301 fsqlpellrl dlsynaiqyi pgnyfhkesk lkyfsaagnk lfsitntfsv trrmevlnls
      361 mnfiedleds lfnmpalkkl tlrnnrikfi rdgtfrsnsy ikhldlsgnd lkwvgidsfe
      421 rlfslevllm tgtsvmslng slhelpklrh lfihdnrlkk lrvvdfansp qivsidakrn
      481 nitdvqgafr kldnlkslsl rrnrlksihr asfpnkldrl ktlvldenpl fcdcqlswli
      541 etapsllwkg vpvchgpdwn rgkqlyslsa dnltewfanc sqscncvchk ntsfsaailv
      601 ncsrrglqal pkefpwktyl ldlswnrlrq ldnrlvhktp sldsllmann llsdigtgvi
      661 prstekldlt knrlrrfplg lvsertvasv wlsgnpwkcs cedypfrqwa eshsyairdy
      721 netlceqgqn lltsgkkfmd lgekdlcpsq kvflilaygv psmvlvvtam aavtaylkyk
      781 heirvwlyar gicrslqcik eddvdedkdf dvflsfsskd rewaysellp kveangfsvc
      841 tydrnfkggf liqdivqeav ccsrrtllvv tqnyvesewc rwefrvaqhr aledninrli
      901 vvvvgdvrpe gvdeelqrym qetnylrwge ahfwdkllys lpkrdartrv ipnmhplthm
      961 atpai
//
LOCUS       XP_077502889             965 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113595 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502889
VERSION     XP_077502889.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646763.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..965
                     /product="uncharacterized protein LOC144113595 isoform X2"
                     /calculated_mol_wt=109998
     Region          75..92
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          96..119
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <112..420
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          120..142
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          165..188
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          189..212
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          213..235
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          236..259
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          260..306
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          307..330
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          331..353
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          352..510
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     Region          354..376
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          377..400
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          401..422
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          425..447
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          448..494
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          495..515
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          520..572
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          528..572
                     /region_name="LRRCT"
                     /note="Leucine rich repeat C-terminal domain; smart00082"
                     /db_xref="CDD:214507"
     Region          565..604
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          573..641
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          580..620
                     /region_name="LRRNT"
                     /note="Leucine rich repeat N-terminal domain; smart00013"
                     /db_xref="CDD:214470"
     Region          642..663
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          813..942
                     /region_name="TIR"
                     /note="Toll - interleukin 1 - resistance; smart00255"
                     /db_xref="CDD:214587"
     CDS             1..965
                     /gene="LOC144113595"
                     /coded_by="XM_077646763.1:72..2969"
                     /db_xref="GeneID:144113595"
ORIGIN      
        1 mrtaaqltla glvliaalsr rqceaaglgg hcpisldwca cmvvrrgprv lcnatadgda
       61 vsgdlsrlag famdslildg vnlttlprry fvnltvkalv vrntpmqste edsfdglthl
      121 ttldmshnrl rtiplgltaf ptlkilrlpd nyiadfknlp plenlteldl sknliekldg
      181 nvlkdfpklq rvwlannlii flstsffkga knvtfvdlhn nmltniytaf qglhhlmevd
      241 lsrnsiigid klstgttprl kilrasynsi swlsrfgpvn ialetielth cniselhret
      301 fsqlpellrl dlsynaiqyi pgnyfhkesk lkyfsaagnk lfsitntfsv trrmevlnls
      361 mnfiedleds lfnmpalkkl tlrnnrikfi rdgtfrsnsy ikhldlsgnd lkwvgidsfe
      421 rlfslevllm tgtsvmslng slhelpklrh lfihdnrlkk lrvvdfansp qivsidakrn
      481 nitdvqgafr kldnlkslsl rrnrlksihr asfpnkldrl ktlvldenpl fcdcqlswli
      541 etapsllwkg vpvchgpdwn rgkqlyslsa dnltewfanc sqscncvchk ntsfsaailv
      601 ncsrrglqal pkefpwktyl ldlswnrlrq ldnrlvhktp sldsllmann llsdigtgvi
      661 prstekldlt knrlrrfplg lvsertvasv wlsgnpwkcs cedypfrqwa eshsyairdy
      721 netlceqgqn lltsgkkfmd lgekdlcpsq kvflilaygv psmvlvvtam aavtaylkyk
      781 heirvwlyar gicrslqcik eddvdedkdf dvflsfsskd rewaysellp kveangfsvc
      841 tydrnfkggf liqdivqeav ccsrrtllvv tqnyvesewc rwefrvaqhr aledninrli
      901 vvvvgdvrpe gvdeelqrym qetnylrwge ahfwdkllys lpkrdartrv ipnmhplthm
      961 atpai
//
LOCUS       XP_077502890             965 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113595 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502890
VERSION     XP_077502890.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646764.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..965
                     /product="uncharacterized protein LOC144113595 isoform X2"
                     /calculated_mol_wt=109998
     Region          75..92
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          96..119
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <112..420
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          120..142
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          165..188
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          189..212
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          213..235
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          236..259
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          260..306
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          307..330
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          331..353
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          352..510
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     Region          354..376
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          377..400
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          401..422
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          425..447
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          448..494
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          495..515
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          520..572
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          528..572
                     /region_name="LRRCT"
                     /note="Leucine rich repeat C-terminal domain; smart00082"
                     /db_xref="CDD:214507"
     Region          565..604
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          573..641
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          580..620
                     /region_name="LRRNT"
                     /note="Leucine rich repeat N-terminal domain; smart00013"
                     /db_xref="CDD:214470"
     Region          642..663
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          813..942
                     /region_name="TIR"
                     /note="Toll - interleukin 1 - resistance; smart00255"
                     /db_xref="CDD:214587"
     CDS             1..965
                     /gene="LOC144113595"
                     /coded_by="XM_077646764.1:101..2998"
                     /db_xref="GeneID:144113595"
ORIGIN      
        1 mrtaaqltla glvliaalsr rqceaaglgg hcpisldwca cmvvrrgprv lcnatadgda
       61 vsgdlsrlag famdslildg vnlttlprry fvnltvkalv vrntpmqste edsfdglthl
      121 ttldmshnrl rtiplgltaf ptlkilrlpd nyiadfknlp plenlteldl sknliekldg
      181 nvlkdfpklq rvwlannlii flstsffkga knvtfvdlhn nmltniytaf qglhhlmevd
      241 lsrnsiigid klstgttprl kilrasynsi swlsrfgpvn ialetielth cniselhret
      301 fsqlpellrl dlsynaiqyi pgnyfhkesk lkyfsaagnk lfsitntfsv trrmevlnls
      361 mnfiedleds lfnmpalkkl tlrnnrikfi rdgtfrsnsy ikhldlsgnd lkwvgidsfe
      421 rlfslevllm tgtsvmslng slhelpklrh lfihdnrlkk lrvvdfansp qivsidakrn
      481 nitdvqgafr kldnlkslsl rrnrlksihr asfpnkldrl ktlvldenpl fcdcqlswli
      541 etapsllwkg vpvchgpdwn rgkqlyslsa dnltewfanc sqscncvchk ntsfsaailv
      601 ncsrrglqal pkefpwktyl ldlswnrlrq ldnrlvhktp sldsllmann llsdigtgvi
      661 prstekldlt knrlrrfplg lvsertvasv wlsgnpwkcs cedypfrqwa eshsyairdy
      721 netlceqgqn lltsgkkfmd lgekdlcpsq kvflilaygv psmvlvvtam aavtaylkyk
      781 heirvwlyar gicrslqcik eddvdedkdf dvflsfsskd rewaysellp kveangfsvc
      841 tydrnfkggf liqdivqeav ccsrrtllvv tqnyvesewc rwefrvaqhr aledninrli
      901 vvvvgdvrpe gvdeelqrym qetnylrwge ahfwdkllys lpkrdartrv ipnmhplthm
      961 atpai
//
LOCUS       XP_077502891             988 aa            linear   INV 25-NOV-2025
DEFINITION  toll-like receptor 2 type-2 [Amblyomma americanum].
ACCESSION   XP_077502891
VERSION     XP_077502891.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646765.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..988
                     /product="toll-like receptor 2 type-2"
                     /calculated_mol_wt=112771
     Region          105..160
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          105..124
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          126..149
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <142..450
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          150..172
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          195..218
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          219..242
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          243..265
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          266..289
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          290..336
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          337..360
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          353..>709
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          361..383
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          384..406
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          407..430
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          431..452
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          478..501
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          502..524
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          525..545
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          558..602
                     /region_name="LRRCT"
                     /note="Leucine rich repeat C-terminal domain; smart00082"
                     /db_xref="CDD:214507"
     Region          595..634
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          603..671
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          672..693
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          836..965
                     /region_name="TIR"
                     /note="Toll - interleukin 1 - resistance; smart00255"
                     /db_xref="CDD:214587"
     CDS             1..988
                     /gene="LOC144113597"
                     /coded_by="XM_077646765.1:16..2982"
                     /db_xref="GeneID:144113597"
ORIGIN      
        1 mklcfvqpea svrshkdets vltwiigcag mrtgaqltla glvliaalnr rqceaaelgg
       61 hcpisldwca cmvvrrgprv lcnatadgda vsddlsrlag famdslildg vnlttlprhy
      121 fvnltvkalv vrntpmqste edsfeglthl ttldmshnrl raiprgltav ptlkilrlpe
      181 nyiadfknlp plenltdldl sknliekldg nvlkdfpklk rvwlannlii flstsffkga
      241 knvtfvdlhn nmltnvhtaf qglrhlteld lsrnsitdid klstgttytl rilrasynsi
      301 swlsrigpfn illetielth cniselrret fsqlpellrl dlsynaiqyi pgncfhkesk
      361 lkyfsaagnk lfsitntfsv trrmevlnls mnfiedleds lfnmpalkel tlrnnrikfi
      421 rdgtfrsnsy ikhldlsgnd lnwvgidsfe rlfslevllm tgtsvmslng slhelpklrh
      481 lfihdnrlkk lraadfknsp qivsidakrn nitdvqgafr kldnlkslsl rrnrlksihr
      541 asfpnkldrl ktlvldenpl fcdcqlswli etapsllwkg vpvchgpdwn rgkqlyslsa
      601 dnltewfanc sqscncvchk ntsfsaailv ncsrrglqal pkefpwktyl ldlswnrlrq
      661 ldnrlvhktp sldsllmann llsdigtgvi prstekldlt knrlrrfplg lvsertvasv
      721 wlsgnpwkcs cedypfrqwa eshsyairdy netlceqgkk fidlgekdlc psrkvfiila
      781 ygvpslvlmv tamaavtayl kykheirvwl yargicrslk cikeddvded kdfdvflsfs
      841 skdrewayse llpkveangf svctydrnfk ggfliqdivq eavccsrrtl lvvtqnyves
      901 ewcrwefrva qhralednin rlivvvvgdv rpegvdeelq rymqetnylr wgeahfwdkl
      961 lyslpkrdar trvipnmhpl trmatpai
//
LOCUS       XP_077502892             974 aa            linear   INV 25-NOV-2025
DEFINITION  toll-like receptor Tollo [Amblyomma americanum].
ACCESSION   XP_077502892
VERSION     XP_077502892.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646766.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..974
                     /product="toll-like receptor Tollo"
                     /calculated_mol_wt=109301
     Region          104..127
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          128..469
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          128..153
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          173..196
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          197..220
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          221..243
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          244..267
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          268..290
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          291..314
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          315..338
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          324..>705
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          339..361
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          362..384
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          385..408
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          409..432
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          433..455
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          456..476
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          480..502
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          503..528
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          531..>577
                     /region_name="PCC"
                     /note="polycystin cation channel protein; TIGR00864"
                     /db_xref="CDD:188093"
     Region          576..649
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          650..671
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          672..694
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          703..>734
                     /region_name="LRRCT"
                     /note="Leucine rich repeat C-terminal domain; smart00082"
                     /db_xref="CDD:214507"
     Region          817..951
                     /region_name="TIR"
                     /note="Toll - interleukin 1 - resistance; smart00255"
                     /db_xref="CDD:214587"
     CDS             1..974
                     /gene="LOC144113598"
                     /coded_by="XM_077646766.1:150..3074"
                     /db_xref="GeneID:144113598"
ORIGIN      
        1 mpssttrglf qvdslacalv macvfcwsea rnwravnatc peklehcqcv vvkqgartsc
       61 kniarpqeld admarlegii hrrlafdhvq iaelparwft nhsvselsik ncplsdvgea
      121 amsgidrlsr vvmenvqlet vprglsaakr lrwlqirksg vrflrgvlsl avlseldlrn
      181 naievideey lsgvpnlqhl llsgnkiqhl spvlfkatrk lrtvefrtnn ittvstmfdd
      241 lryleavdls hnmitdiqel lasrlpylki lklgnnrisv vtevalpnvm ikelllndne
      301 itqvrngafa alssmtrldi snnsiaqmee svlpttsnle yltlsrtgli nvagsfskqr
      361 hikdlvlsln rieditdaft glemlrtvsl rsnlvthvpd gafsdssgll einlsdsrir
      421 wvgrnafkel ltlnklilsg nqllslngsv anlpklhyld asfnaiqsle rgeftnngrl
      481 tfiqlganni snvrgaftga tdlvglvlrg nalkllrrsd fpkrfkgkpt ltlndnplmc
      541 dcrlswlvgp gsevqmrnnp icegppwlag rfllnltedd lvrweadcep acrcgcreds
      601 lgkrtvlvnc sssalegaps tfpegttqle lldnrieald galvndaprl rvlslrknll
      661 ssvnvttipe evqsldlsyn rlkrfpytlv sqrklttlrl sgnpfvcdca dyrfrqwiea
      721 hrkvivdaqd ivcaehtnpl vslkaflslg qkqlcpvaip ravayllpvl valamglais
      781 aaylryrrel kvwlyarglc lslqcvkede ldeaklfdvf lsfssrdgdw vhaqllpgve
      841 slgfsvctye rnfkggfllq diirdavacs rrtlllltrn fvesewcrwe frlayqrale
      901 dhvnrlvvvl vddvapdsmd ddlrayvraa nyirwgehnf wdkllyslpk kdakrklite
      961 rteqypmthi sqag
//
LOCUS       XP_077502893             974 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113599 [Amblyomma americanum].
ACCESSION   XP_077502893
VERSION     XP_077502893.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646767.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..974
                     /product="uncharacterized protein LOC144113599"
                     /calculated_mol_wt=110166
     Region          104..127
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <114..422
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          128..150
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          151..172
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          173..196
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          197..220
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          221..243
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          244..267
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          268..290
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          292..314
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          315..338
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          331..>705
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          339..361
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          362..384
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          385..408
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          409..432
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          433..455
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          456..479
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          480..502
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          503..523
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          539..649
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          544..620
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          650..671
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          672..694
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          817..951
                     /region_name="TIR"
                     /note="Toll - interleukin 1 - resistance; smart00255"
                     /db_xref="CDD:214587"
     CDS             1..974
                     /gene="LOC144113599"
                     /coded_by="XM_077646767.1:118..3042"
                     /db_xref="GeneID:144113599"
ORIGIN      
        1 mrastaktas rvdlfasalv vvvcalcsve arqwrkvnvt cpklerchct tvnqgaltlc
       61 rdvsdaseld admatlegvi hrkltfdqvq itelparwft nhtvimlivn ncplrdigea
      121 alngidslsr vmlendqles vprgltaakh lhwlevrknr isflqgvlfl pelyeldlry
      181 naievidday lsgmpnlqil rlssnriqhl spdlfkntgk lkivefrnnr itainnafng
      241 mryletldls dnmitdadgv fssrmpylki lkldhnriqv vtelasynam ikevylndne
      301 isqvdfgafa aieamtrlyl nnnnisrlhg svfhveskle ilsladnalt svagtfsrtr
      361 rmsdlrlfsn riedisdafk altllrklsl mdnlvthvpd gtfsdnsgll einlsnnrir
      421 wvgkyafkgi vtlekvllqn nrllalngsf knlpklryvd asynsirsle kgeftnngrl
      481 tsihlaanni stvqwaftga snltvlvlrk nrvellrrsd farvlrvrpa ftiddnpllc
      541 dcrlawlvrp daevqmrnya acegppwlkg klladltqnd ltkwqqdcqp gcqcdcvedg
      601 igdraihvdc ssatlnrapk vypegttqle lrnnrleeld dalvngaphl qilslknnll
      661 sslnatnvpe klrsldisen rlkrfphalv sqrelrtlrl sgnpfacecs dypfrqwiea
      721 hgnvvldiee vicaessnsm vslkpfvnlg qkqlcpaavp ravavllpvl valamglais
      781 aaylryrrel kvwlyarglc lslqcvkede ldeaklfdvf lsfssrdgdw vhaqllpgve
      841 slgfsvctye rnfkggfllq diirdavacs rrtlllltrn flesewcrwe frlayqrale
      901 dhinrlvvvl vddvaldvld edlrefvrav nyirwgepnf wdkllyslpk kdskrklitd
      961 rtqqypmeht saag
//
LOCUS       XP_077502895             598 aa            linear   INV 25-NOV-2025
DEFINITION  protein PALS2-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502895
VERSION     XP_077502895.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646769.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..598
                     /product="protein PALS2-like isoform X1"
                     /calculated_mol_wt=67741
     Region          34..79
                     /region_name="L27"
                     /note="L27 domain; cl02672"
                     /db_xref="CDD:470651"
     Region          91..138
                     /region_name="L27"
                     /note="L27 domain; pfam02828"
                     /db_xref="CDD:460717"
     Region          161..238
                     /region_name="PDZ_MPP6-MPP2-like"
                     /note="PDZ domain of membrane palmitoylated protein 6
                     (MPP6), MPP2, and related domains; cd10832"
                     /db_xref="CDD:467268"
     Site            order(172..178,187,190,218,222,225..226)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467268"
     Region          250..309
                     /region_name="SH3_MPP"
                     /note="Src Homology 3 domain of Membrane Protein,
                     Palmitoylated (or MAGUK p55 subfamily member) proteins;
                     cd11862"
                     /db_xref="CDD:212796"
     Site            order(252,276..277)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212796"
     Site            order(255,257,260,271,289..290,306,308..309)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212796"
     Region          371..585
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..598
                     /gene="LOC144113600"
                     /coded_by="XM_077646769.1:299..2095"
                     /db_xref="GeneID:144113600"
ORIGIN      
        1 mvlcggrpga dddgrqllrs teqlnqqkqg tafqhvreni edlngrvgvr dtdliflkgl
       61 mespvmsclv qvqdhleekt ttfkpvstnn vellrdvcqa crrvsgrdaq elanilsgph
      121 fsallnahda vatksyepak plalekeapa mapetlndti rmvgirkndd eplgitvrve
      181 ddnlmiaril aggiidrqgl lhvgdtilev ngvpvhtpeq lqlqlkkaqg svafkilpsy
      241 hdnlptfqcf vkalytyeps kdtllpckei glpfkqgdil qvlnqedpnw wqarrvsasg
      301 paglipsqel eerrrafvrp eydyatktkm cgtkitkkkr ktmyeskasa efekaellly
      361 eevarmppfe rktvvlvgar gvgrrslknk lisydparfg tplphtsrpi retetdgkvy
      421 yfvsrevmea diadnkylew gehsghlygt kldtlravir sgkmcvvdcs pqylkilntp
      481 efmpyvvfia apqlehfrqm heygrhhgyg ssrnltfdra msrhgsrrar tleslaslqe
      541 dedlkrtiee saqlqrayer yfdtilvnnn fektfeqlkd vvdslstepq wvpiswvy
//
LOCUS       XP_077502896             569 aa            linear   INV 25-NOV-2025
DEFINITION  protein PALS2-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502896
VERSION     XP_077502896.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646770.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..569
                     /product="protein PALS2-like isoform X2"
                     /calculated_mol_wt=64588
     Region          5..50
                     /region_name="L27"
                     /note="L27 domain; cl02672"
                     /db_xref="CDD:470651"
     Region          62..109
                     /region_name="L27"
                     /note="L27 domain; pfam02828"
                     /db_xref="CDD:460717"
     Region          132..209
                     /region_name="PDZ_MPP6-MPP2-like"
                     /note="PDZ domain of membrane palmitoylated protein 6
                     (MPP6), MPP2, and related domains; cd10832"
                     /db_xref="CDD:467268"
     Site            order(143..149,158,161,189,193,196..197)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467268"
     Region          221..280
                     /region_name="SH3_MPP"
                     /note="Src Homology 3 domain of Membrane Protein,
                     Palmitoylated (or MAGUK p55 subfamily member) proteins;
                     cd11862"
                     /db_xref="CDD:212796"
     Site            order(223,247..248)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212796"
     Site            order(226,228,231,242,260..261,277,279..280)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212796"
     Region          342..556
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..569
                     /gene="LOC144113600"
                     /coded_by="XM_077646770.1:168..1877"
                     /db_xref="GeneID:144113600"
ORIGIN      
        1 maafqhvren iedlngrvgv rdtdliflkg lmespvmscl vqvqdhleek tttfkpvstn
       61 nvellrdvcq acrrvsgrda qelanilsgp hfsallnahd avatksyepa kplalekeap
      121 amapetlndt irmvgirknd deplgitvrv eddnlmiari laggiidrqg llhvgdtile
      181 vngvpvhtpe qlqlqlkkaq gsvafkilps yhdnlptfqc fvkalytyep skdtllpcke
      241 iglpfkqgdi lqvlnqedpn wwqarrvsas gpaglipsqe leerrrafvr peydyatktk
      301 mcgtkitkkk rktmyeskas aefekaelll yeevarmppf erktvvlvga rgvgrrslkn
      361 klisydparf gtplphtsrp iretetdgkv yyfvsrevme adiadnkyle wgehsghlyg
      421 tkldtlravi rsgkmcvvdc spqylkilnt pefmpyvvfi aapqlehfrq mheygrhhgy
      481 gssrnltfdr amsrhgsrra rtleslaslq ededlkrtie esaqlqraye ryfdtilvnn
      541 nfektfeqlk dvvdslstep qwvpiswvy
//
LOCUS       XP_077502897             538 aa            linear   INV 25-NOV-2025
DEFINITION  protein PALS2-like [Amblyomma americanum].
ACCESSION   XP_077502897
VERSION     XP_077502897.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646771.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..538
                     /product="protein PALS2-like"
                     /calculated_mol_wt=60937
     Region          31..78
                     /region_name="L27"
                     /note="L27 domain; pfam02828"
                     /db_xref="CDD:460717"
     Region          101..178
                     /region_name="PDZ_MPP6-MPP2-like"
                     /note="PDZ domain of membrane palmitoylated protein 6
                     (MPP6), MPP2, and related domains; cd10832"
                     /db_xref="CDD:467268"
     Site            order(112..118,127,130,158,162,165..166)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467268"
     Region          190..249
                     /region_name="SH3_MPP"
                     /note="Src Homology 3 domain of Membrane Protein,
                     Palmitoylated (or MAGUK p55 subfamily member) proteins;
                     cd11862"
                     /db_xref="CDD:212796"
     Site            order(192,216..217)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212796"
     Site            order(195,197,200,211,229..230,246,248..249)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212796"
     Region          311..525
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..538
                     /gene="LOC144113602"
                     /coded_by="XM_077646771.1:358..1974"
                     /db_xref="GeneID:144113602"
ORIGIN      
        1 mespvmsclv qvqdhleekt ttfkpvstnn vellrdvcqa crrvsgrdaq elanilsgph
       61 fsallnahda vatksyepak plalekeapa mapetlndti rmvgirkndd eplgitvrae
      121 ddnlmiaril aggiidrqgl lhvgdtilev ngvpvhtpeq lqlqlkkaqg svafkilpsy
      181 hdnlptfqcf vkalytyeps kdtllpckei glpfkqgdil qvlnqedpnw wqarrvsasg
      241 paglipsqel eerrrafvrp eydyatktkm cgtkvtkkkr ktmyeskasa efekaellly
      301 eevarmppfe rktvvlvgar gvgrrslknk lisydptrfg tplphtsrpi retetdgkvy
      361 yfvsrevmea diadnkyleg gehsghlygt kldtlravir sgkmcvvdcs pqylkilntp
      421 efmpylvfia apqlehfrqm heygrhhgyg ssrnltfdra msrhgsrrar tleslaslqe
      481 dedlkrtiee saqlqrayer yfdtilvnnn fektfeqlkd vidslstepq wvpisway
//
LOCUS       XP_077502898            1503 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113603 [Amblyomma americanum].
ACCESSION   XP_077502898
VERSION     XP_077502898.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646772.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1503
                     /product="uncharacterized protein LOC144113603"
                     /calculated_mol_wt=170637
     Region          2..44
                     /region_name="Rho_N"
                     /note="Rho termination factor, N-terminal domain;
                     smart00959"
                     /db_xref="CDD:198027"
     Region          210..300
                     /region_name="SCAN"
                     /note="SCAN domain; pfam02023"
                     /db_xref="CDD:460417"
     Region          361..378
                     /region_name="zf-CCHC"
                     /note="Zinc knuckle; pfam00098"
                     /db_xref="CDD:395050"
     Region          649..705
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          725..820
                     /region_name="rve"
                     /note="Integrase core domain; pfam00665"
                     /db_xref="CDD:459897"
     Region          1112..1287
                     /region_name="RT_LTR"
                     /note="Reverse transcriptases (RTs) from retrotransposons
                     and retroviruses which have long terminal repeats (LTRs)
                     in their DNA copies but not in their RNA template. RT
                     catalyzes DNA replication from an RNA template, and is
                     responsible for the replication of...; cd01647"
                     /db_xref="CDD:238825"
     Site            order(1175..1180,1209..1210,1236,1238..1239,1283..1284)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238825"
     Site            order(1175..1180,1209,1238)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238825"
     Site            1210
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238825"
     Region          1383..1503
                     /region_name="RNase_HI_RT_Ty3"
                     /note="Ty3/Gypsy family of RNase HI in long-term repeat
                     retroelements; cd09274"
                     /db_xref="CDD:260006"
     Site            order(1387..1390,1424..1426,1429,1454,1487,1501)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260006"
     CDS             1..1503
                     /gene="LOC144113603"
                     /coded_by="XM_077646772.1:1545..6056"
                     /db_xref="GeneID:144113603"
ORIGIN      
        1 mdlkslrkpk llelarelgl dvsdklrkpe llrailelea eddelsecle tieereqkek
       61 eererkeqke kdererkeqk ekeererkeq kekekeerdr qhalemkrle vemerarngs
      121 qahgagervs fkmtdlmrpf klgediglfl vnfertcekq gfsretwpqr lltllpaeaa
      181 dvvarlkree aedfdqvkss llkkyrlsae afrrkfrene kgrnesytef ayrlmsnmqe
      241 wlkeekafgd hekilqcfgl eqfysrlpen vrywvldrpd vstvakaael aeefvtrrar
      301 gakdgqkgef gskserpkft pmrargdtrs adasesspte rketaaaeae rrklfearqa
      361 rvcytcqkpg hfsaqcpetk tkvvflslcs tdenmkllep ymrdflvngk ecrvlrdsaa
      421 tmdvvhpsyv epdmftgeca wikqaveahs vclpvakvli egpfgaiete aavssmlppq
      481 ypylfsnrsd hllrekgllf geasvqaltr srvrelaaka vvagptlssn ekgseaqqad
      541 iqstselnki epvalkapgt geempdtgkl kelstdllia ptsdglnrll kvswsaliae
      601 qkkdgslenm rcnvkegiak knvrfvergg vlyrkyldrr gvefdqlivp qcyrqdllrl
      661 shggswsghl gvkktkdrll qeyywpgcfr dvetfvrtcd tcqrvgkpgd ksraplklvp
      721 iitepfrrlv idtvgplpvt atgyrhiltv icpatkfpea vplkdlssve ivnallsifa
      781 rvgfpaeiqs dqgtvftsal ttaflercgv kllhssvyhp qsnsveklhs vmkrvlralc
      841 feqqsdwelc lpgvmfalrt aphaatgfsp aelvygrslr splrmlrdsw egrgddpvvv
      901 eyvlsllerl rraqelsgva makaqqrakv yydrtararr fevgdevmil rtslknkldv
      961 qwegparivq klsdvnyvvs lpgkrkaqqv yhcnllkpyr qreavvcmmv nvpeelpvel
     1021 pglgsvtnre dtdqvisdli ikaplsaeqr telhqllqef qgqfserpgr tsvlthdiel
     1081 tspepvrska yrvsprqrdi meaevkkmlq lgvieagesd ytsplilvev pgkeprpcvd
     1141 yrrlnsitkd qiypipniee rlekvssaqf istldlvrgy wqvplteeas ryaafispmg
     1201 tfrpkvlsfg lkntpycfss lmdkvlrgqe efalpylddv aifsaswseh mahlravltr
     1261 lreagltvka pkcqlaqaev vylghvigqg rrrpseikva avrdfpqprt ktdirsflgv
     1321 agyyqryipr ysdiaapltd alrktepqtv vwsetkeraf salksaltsq pvlrspdytk
     1381 gfvvqcdase rgmgvvlcqr dngevehpvl yasrkltcre qaysatekec aclvwavqkl
     1441 scylagsrfi ietdhcplqw lqtispkngr llrwslalqq ysfevrykkg slngnadgls
     1501 rsp
//
LOCUS       XP_077502899             378 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113604 [Amblyomma americanum].
ACCESSION   XP_077502899
VERSION     XP_077502899.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646773.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..378
                     /product="uncharacterized protein LOC144113604"
                     /calculated_mol_wt=43025
     Region          5..225
                     /region_name="HAUS6_N"
                     /note="HAUS augmin-like complex subunit 6 N-terminus;
                     pfam14661"
                     /db_xref="CDD:464244"
     Region          143..>329
                     /region_name="CwlO1"
                     /note="Uncharacterized N-terminal coiled-coil domain of
                     peptidoglycan hydrolase CwlO [Function unknown]; COG3883"
                     /db_xref="CDD:443091"
     CDS             1..378
                     /gene="LOC144113604"
                     /coded_by="XM_077646773.1:140..1276"
                     /db_xref="GeneID:144113604"
ORIGIN      
        1 medvllvcld alhfkppddt kllqehfrgr qapatvrqvl hflyytydkk avesrmrniw
       61 pvktgesdkr freeitqwis tlqgdelatp yiqefsaall lrpgtpqgsk flaslsvfvl
      121 hyklkdsvhf sspfspaerp awklemvkka tiqtqsqnaa fkeeldafes ekakmqslle
      181 kkrsdlelsi khlqaqvqsf esddtlmsle klcrklraee rdskktleii neavetfsrs
      241 krvidlsslt dkqskflsss glvdvvgtfk dctelakkls aqfvkegctq qdleavklrv
      301 aalratlrrl qeqretakam kaeidqrlsa gckkpfvvln dkdidemcrp aaqpatepdv
      361 eefvnellav hrsqnrnq
//
LOCUS       XP_077502900             378 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113604 [Amblyomma americanum].
ACCESSION   XP_077502900
VERSION     XP_077502900.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646774.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..378
                     /product="uncharacterized protein LOC144113604"
                     /calculated_mol_wt=43025
     Region          5..225
                     /region_name="HAUS6_N"
                     /note="HAUS augmin-like complex subunit 6 N-terminus;
                     pfam14661"
                     /db_xref="CDD:464244"
     Region          143..>329
                     /region_name="CwlO1"
                     /note="Uncharacterized N-terminal coiled-coil domain of
                     peptidoglycan hydrolase CwlO [Function unknown]; COG3883"
                     /db_xref="CDD:443091"
     CDS             1..378
                     /gene="LOC144113604"
                     /coded_by="XM_077646774.1:141..1277"
                     /db_xref="GeneID:144113604"
ORIGIN      
        1 medvllvcld alhfkppddt kllqehfrgr qapatvrqvl hflyytydkk avesrmrniw
       61 pvktgesdkr freeitqwis tlqgdelatp yiqefsaall lrpgtpqgsk flaslsvfvl
      121 hyklkdsvhf sspfspaerp awklemvkka tiqtqsqnaa fkeeldafes ekakmqslle
      181 kkrsdlelsi khlqaqvqsf esddtlmsle klcrklraee rdskktleii neavetfsrs
      241 krvidlsslt dkqskflsss glvdvvgtfk dctelakkls aqfvkegctq qdleavklrv
      301 aalratlrrl qeqretakam kaeidqrlsa gckkpfvvln dkdidemcrp aaqpatepdv
      361 eefvnellav hrsqnrnq
//
LOCUS       XP_077502901             155 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113605 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502901
VERSION     XP_077502901.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646775.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..155
                     /product="uncharacterized protein LOC144113605 isoform X1"
                     /calculated_mol_wt=16900
     CDS             1..155
                     /gene="LOC144113605"
                     /coded_by="XM_077646775.1:88..555"
                     /db_xref="GeneID:144113605"
ORIGIN      
        1 mavadvsaam lvlidgvetr ayalldehgt hmenddgfly etegglrisv lrveepirnt
       61 ppptvpavve dgsrlapspl rqttppvvwq ptpspgpscy spppsghger dawndrksrf
      121 litkykkpri alaervalgk sqrvkcrndt clcar
//
LOCUS       XP_077502902             144 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113605 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502902
VERSION     XP_077502902.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646776.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..144
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..144
                     /product="uncharacterized protein LOC144113605 isoform X2"
                     /calculated_mol_wt=15595
     CDS             1..144
                     /gene="LOC144113605"
                     /coded_by="XM_077646776.1:85..519"
                     /db_xref="GeneID:144113605"
ORIGIN      
        1 mavadvsaam lvlidgvetr ayalldehgt hmenddgfly etegglrisv lrveepirnt
       61 ppptvpavve dgsrlapspl rqttppvvwq ptpspgpscy spppsghger dawndrksrf
      121 litkykkpri alaervalgn sprc
//
LOCUS       XP_077502903             538 aa            linear   INV 25-NOV-2025
DEFINITION  protein PALS2-like [Amblyomma americanum].
ACCESSION   XP_077502903
VERSION     XP_077502903.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646777.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..538
                     /product="protein PALS2-like"
                     /calculated_mol_wt=60991
     Region          31..78
                     /region_name="L27"
                     /note="L27 domain; pfam02828"
                     /db_xref="CDD:460717"
     Region          101..178
                     /region_name="PDZ_MPP6-MPP2-like"
                     /note="PDZ domain of membrane palmitoylated protein 6
                     (MPP6), MPP2, and related domains; cd10832"
                     /db_xref="CDD:467268"
     Site            order(112..118,127,130,158,162,165..166)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467268"
     Region          190..249
                     /region_name="SH3_MPP"
                     /note="Src Homology 3 domain of Membrane Protein,
                     Palmitoylated (or MAGUK p55 subfamily member) proteins;
                     cd11862"
                     /db_xref="CDD:212796"
     Site            order(192,216..217)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212796"
     Site            order(195,197,200,211,229..230,246,248..249)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212796"
     Region          311..525
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..538
                     /gene="LOC144113608"
                     /coded_by="XM_077646777.1:224..1840"
                     /db_xref="GeneID:144113608"
ORIGIN      
        1 mespvmsclv qvqdhleekt itfkpvstnn vellrdvcqa crrvsgrdaq elanilsgph
       61 fsallnahda vatksyepak plalekeapa mapetlndti rmvgirkndd eplgitvrae
      121 ddnlmiaril aggiidrqgl lhvgdtilev ngvpvhtpeq lqlqlkkaqg svafkilpsy
      181 hdnlptfqcf vkalytyeps kdtllpckei glpfkqgdil qvlnqedpnw wqarrvsasg
      241 paglipsqel eerrrafvrp eydyatktkm cgtkitkkkr ktmyeskasa efekaellly
      301 eevarmppfe rktvvlvgar gvgrrslknk lisydptrfg tplphtsrpi retetdgkvy
      361 yfvsrevmea diadnkyleg gehsghlygt kldtlravir sgkmcvvdcs pqylkilntp
      421 efmpylvfia apqlehfrqm heygrhhgyg ssrnltfdra msrhgsrrar tleslaslqe
      481 dedlkrtiee saqlqrayer yfdtilvnnn fektfeqlkd vidslstepq wvpiswvy
//
LOCUS       XP_077502904             540 aa            linear   INV 25-NOV-2025
DEFINITION  protein PALS2-like [Amblyomma americanum].
ACCESSION   XP_077502904
VERSION     XP_077502904.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646778.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..540
                     /product="protein PALS2-like"
                     /calculated_mol_wt=61305
     Region          31..78
                     /region_name="L27"
                     /note="L27 domain; pfam02828"
                     /db_xref="CDD:460717"
     Region          101..180
                     /region_name="PDZ_MPP6-MPP2-like"
                     /note="PDZ domain of membrane palmitoylated protein 6
                     (MPP6), MPP2, and related domains; cd10832"
                     /db_xref="CDD:467268"
     Site            order(112..118,127,130,158,164,167..168)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467268"
     Region          192..251
                     /region_name="SH3_MPP"
                     /note="Src Homology 3 domain of Membrane Protein,
                     Palmitoylated (or MAGUK p55 subfamily member) proteins;
                     cd11862"
                     /db_xref="CDD:212796"
     Site            order(194,218..219)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212796"
     Site            order(197,199,202,213,231..232,248,250..251)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212796"
     Region          313..527
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..540
                     /gene="LOC144113613"
                     /coded_by="XM_077646778.1:447..2069"
                     /db_xref="GeneID:144113613"
ORIGIN      
        1 mespvmsclv qvqdhleekt ttfkpvstnn vellrdvcqa crrvsgrdaq elanilsgph
       61 fsallnahda vatksyepak plalekeapa mapetlndti rmvgirkndd eplgitvrve
      121 ddnlmiaril aggiidrqgl lhvgdtilev ngvpvhtpeq lqlqlqlkka qgsvafkilp
      181 syhdnlptfq cfvkalytye pskdtllpck eiglpfkqgd ilqvlnqedp nwwqarrvsa
      241 sgpaglipsq eleerrrafv rpeydyatkt kmcgtkvtkk krktmyeska saefekaell
      301 lyeevarmpp ferktvvlvg argvgrrslk nklisydpar fgtplphtsr piretetdgk
      361 vyyfvsrevm eadiadnkyl ewgehsghly gtkldtlrav irsgkmcvvd cspqylkiln
      421 tpefmpyvvf iaapqlehfr qmheygrhhg ygssrnltfd ramsrhgsrr artleslasl
      481 qededlkrti eesaqlqray eryfdtilvn nnfektfeql kdvvdslste pqwvpiswvy
//
LOCUS       XP_077502905             333 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113614 [Amblyomma americanum].
ACCESSION   XP_077502905
VERSION     XP_077502905.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646779.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..333
                     /product="uncharacterized protein LOC144113614"
                     /calculated_mol_wt=37561
     Region          5..>136
                     /region_name="HAUS6_N"
                     /note="HAUS augmin-like complex subunit 6 N-terminus;
                     pfam14661"
                     /db_xref="CDD:464244"
     Region          143..>281
                     /region_name="CwlO1"
                     /note="Uncharacterized N-terminal coiled-coil domain of
                     peptidoglycan hydrolase CwlO [Function unknown]; COG3883"
                     /db_xref="CDD:443091"
     CDS             1..333
                     /gene="LOC144113614"
                     /coded_by="XM_077646779.1:140..1141"
                     /db_xref="GeneID:144113614"
ORIGIN      
        1 medvllvcld alhfkppddt kllqehfrgr qapatvrqvl hflyytydkk avesrmrniw
       61 pvktgesdkr freeitqwis tlqgdelatp yiqefsaall lrpgtpqgsk flaslsvfvl
      121 hyklkdsvhf sspfspaerp awklemvkka tiqtqsqnaa fkeeldafes ekaktqslle
      181 kkrsdlelsi khlqaqvqrs savlkvsicp lisssglvdv vgtfkdctel akklsaqfvk
      241 egctqqdlea vklrvaalra tlrrlqeqre takamkaeid qclsagckkp fvvlndkdid
      301 emcrpaaqpa tepdveefvn ellavhrsqn rnq
//
LOCUS       XP_077502906             333 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113614 [Amblyomma americanum].
ACCESSION   XP_077502906
VERSION     XP_077502906.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646780.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..333
                     /product="uncharacterized protein LOC144113614"
                     /calculated_mol_wt=37561
     Region          5..>136
                     /region_name="HAUS6_N"
                     /note="HAUS augmin-like complex subunit 6 N-terminus;
                     pfam14661"
                     /db_xref="CDD:464244"
     Region          143..>281
                     /region_name="CwlO1"
                     /note="Uncharacterized N-terminal coiled-coil domain of
                     peptidoglycan hydrolase CwlO [Function unknown]; COG3883"
                     /db_xref="CDD:443091"
     CDS             1..333
                     /gene="LOC144113614"
                     /coded_by="XM_077646780.1:141..1142"
                     /db_xref="GeneID:144113614"
ORIGIN      
        1 medvllvcld alhfkppddt kllqehfrgr qapatvrqvl hflyytydkk avesrmrniw
       61 pvktgesdkr freeitqwis tlqgdelatp yiqefsaall lrpgtpqgsk flaslsvfvl
      121 hyklkdsvhf sspfspaerp awklemvkka tiqtqsqnaa fkeeldafes ekaktqslle
      181 kkrsdlelsi khlqaqvqrs savlkvsicp lisssglvdv vgtfkdctel akklsaqfvk
      241 egctqqdlea vklrvaalra tlrrlqeqre takamkaeid qclsagckkp fvvlndkdid
      301 emcrpaaqpa tepdveefvn ellavhrsqn rnq
//
LOCUS       XP_077502907             144 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113615 [Amblyomma americanum].
ACCESSION   XP_077502907
VERSION     XP_077502907.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646781.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..144
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..144
                     /product="uncharacterized protein LOC144113615"
                     /calculated_mol_wt=15595
     CDS             1..144
                     /gene="LOC144113615"
                     /coded_by="XM_077646781.1:102..536"
                     /db_xref="GeneID:144113615"
ORIGIN      
        1 mavadvsaam lvlidgvetr ayalldehgt hmenddgfly etegglrisv lrveepirnt
       61 ppptvpavve dgsrlapspl rqttppvvwq ptpspgpscy spppsghger dawndrksrf
      121 litkykkpri alaervalgn sprc
//
LOCUS       XP_077502908             538 aa            linear   INV 25-NOV-2025
DEFINITION  protein PALS2-like [Amblyomma americanum].
ACCESSION   XP_077502908
VERSION     XP_077502908.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646782.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..538
                     /product="protein PALS2-like"
                     /calculated_mol_wt=61100
     Region          31..78
                     /region_name="L27"
                     /note="L27 domain; pfam02828"
                     /db_xref="CDD:460717"
     Region          101..178
                     /region_name="PDZ_MPP6-MPP2-like"
                     /note="PDZ domain of membrane palmitoylated protein 6
                     (MPP6), MPP2, and related domains; cd10832"
                     /db_xref="CDD:467268"
     Site            order(112..118,127,130,158,162,165..166)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467268"
     Region          190..249
                     /region_name="SH3_MPP"
                     /note="Src Homology 3 domain of Membrane Protein,
                     Palmitoylated (or MAGUK p55 subfamily member) proteins;
                     cd11862"
                     /db_xref="CDD:212796"
     Site            order(192,216..217)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212796"
     Site            order(195,197,200,211,229..230,246,248..249)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212796"
     Region          311..525
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..538
                     /gene="LOC144113618"
                     /coded_by="XM_077646782.1:224..1840"
                     /db_xref="GeneID:144113618"
ORIGIN      
        1 mespvmsclv qvqdhleekt ttfkpvstnn vellrdvcqa crrvsgrdaq elanilsgph
       61 fsallnahda vatksyepak plalekeapa mapetlndti rmvgirkndd eplgitvrve
      121 ddnlmiaril aggiidrqgl lhvghtilev ngvpvhtpeq lqlqlkkaqg svafkilpsy
      181 hdnlptfqcf vkalytyeps kdtllpckei glpfkqgdil qvlnqedpnw wqarrvsasg
      241 paglipsqel eerrrafvrp eydyatktkm cgtkvtkkkr ktmyeskasa efekaellly
      301 eevarmppfe rktvvlvgar gvgrrslknk lisydparfg tplphtsrpi retetdgkvy
      361 yfvsrevmea diadnkylew gehsghlygt kldtlravir sgkmcvvdcs pqylkilntp
      421 efmpyvvfia apqlehfrqm heygrhhgyg ssrnltfdra msrhgsrrar tleslaslqe
      481 dedlkrtiee saqlqrayer yfdtilvnnn fektfeqlkd vidslstepq wvpiswvy
//
LOCUS       XP_077502909             150 aa            linear   INV 25-NOV-2025
DEFINITION  NPC intracellular cholesterol transporter 2-like [Amblyomma
            americanum].
ACCESSION   XP_077502909
VERSION     XP_077502909.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646783.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..150
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..150
                     /product="NPC intracellular cholesterol transporter
                     2-like"
                     /calculated_mol_wt=15948
     Region          26..146
                     /region_name="Npc2_like"
                     /note="Niemann-Pick type C2 (Npc2) is a lysosomal protein
                     in which a mutation in the gene causes a rare form of
                     Niemann-Pick type C disease, an autosomal recessive lipid
                     storage disorder characterized by accumulation of
                     low-density lipoprotein-derived...; cd00916"
                     /db_xref="CDD:238458"
     Site            order(86,116,120)
                     /site_type="other"
                     /note="putative cholesterol/lipid binding site [chemical
                     binding]"
                     /db_xref="CDD:238458"
     CDS             1..150
                     /gene="LOC144113622"
                     /coded_by="XM_077646783.1:77..529"
                     /db_xref="GeneID:144113622"
ORIGIN      
        1 mevrrqllla alllssaaca lgdwpqcdap dgttvnfeie gckppgtcvl kkgtqanvti
       61 hfkskvasqg vkvkaygvfh evplpfplpq pdacksgvtc pvqaqgsyty rgsfpvksay
      121 psislnvkwe liddasknlv cqlipveiss
//
LOCUS       XP_077502910             148 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113623 [Amblyomma americanum].
ACCESSION   XP_077502910
VERSION     XP_077502910.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646784.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="uncharacterized protein LOC144113623"
                     /calculated_mol_wt=15085
     CDS             1..148
                     /gene="LOC144113623"
                     /coded_by="XM_077646784.1:169..615"
                     /db_xref="GeneID:144113623"
ORIGIN      
        1 mgsspgtrac yaaagiftls gfllsgasva ivmmivrddk ldeqlwtaav lvvgvsfgvl
       61 clgilvcaca mckrmaasqa aldkaeavre qgakrrlppa aafpaghvaa vrvwstagsl
      121 dpllpgqvaa gespseaedd eqpreeva
//
LOCUS       XP_077502911             537 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113624 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502911
VERSION     XP_077502911.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646785.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..537
                     /product="uncharacterized protein LOC144113624 isoform X1"
                     /calculated_mol_wt=57290
     CDS             1..537
                     /gene="LOC144113624"
                     /coded_by="XM_077646785.1:181..1794"
                     /db_xref="GeneID:144113624"
ORIGIN      
        1 mlfnsatyra qrrqpgttma ssrppleggc srvcvillyl sgmlliiagv vaggfivnyp
       61 dilfylavas gsaivlgilf vslsacvcir rrpmpskdaa rkfapptsng vkgvttadfk
      121 easvgsrddl ngsapsarps dgllnialek geffhntsvd psnprraqqk pvtssprttk
      181 aklvrdgavf peppkvseip vgsrsqpdsl rrakvkngkl pkapasvgdf aslglqdddd
      241 avfsateksk psvkgrgkvp ltafkqtsks vsdlddessf esstrapqqr tavrpetpqm
      301 eprlavschn ilseepvrvg iahlrqredp svsrkppvpr kvtgialpgm vqrapgagaa
      361 slsqddlsrv rlkpaeqkpr aplrdqgped gkqrlqhksv pdffpqdggv rltlldssss
      421 sygkssrtta papetplnts lasyadaagp edlhrcntvg lppaplpkpa tsgqqfrvya
      481 pkqdslvpdk qplmaewaas issksdtfss deaecqrhls ssqellmgnv kcletei
//
LOCUS       XP_077502912             527 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113624 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502912
VERSION     XP_077502912.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646786.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..527
                     /product="uncharacterized protein LOC144113624 isoform X2"
                     /calculated_mol_wt=56135
     CDS             1..527
                     /gene="LOC144113624"
                     /coded_by="XM_077646786.1:127..1710"
                     /db_xref="GeneID:144113624"
ORIGIN      
        1 mlevysttma ssrppleggc srvcvillyl sgmlliiagv vaggfivnyp dilfylavas
       61 gsaivlgilf vslsacvcir rrpmpskdaa rkfapptsng vkgvttadfk easvgsrddl
      121 ngsapsarps dgllnialek geffhntsvd psnprraqqk pvtssprttk aklvrdgavf
      181 peppkvseip vgsrsqpdsl rrakvkngkl pkapasvgdf aslglqdddd avfsateksk
      241 psvkgrgkvp ltafkqtsks vsdlddessf esstrapqqr tavrpetpqm eprlavschn
      301 ilseepvrvg iahlrqredp svsrkppvpr kvtgialpgm vqrapgagaa slsqddlsrv
      361 rlkpaeqkpr aplrdqgped gkqrlqhksv pdffpqdggv rltlldssss sygkssrtta
      421 papetplnts lasyadaagp edlhrcntvg lppaplpkpa tsgqqfrvya pkqdslvpdk
      481 qplmaewaas issksdtfss deaecqrhls ssqellmgnv kcletei
//
LOCUS       XP_077502913             525 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113624 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502913
VERSION     XP_077502913.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646787.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..525
                     /product="uncharacterized protein LOC144113624 isoform X3"
                     /calculated_mol_wt=55827
     CDS             1..525
                     /gene="LOC144113624"
                     /coded_by="XM_077646787.1:134..1711"
                     /db_xref="GeneID:144113624"
ORIGIN      
        1 mepgttmass rppleggcsr vcvillylsg mlliiagvva ggfivnypdi lfylavasgs
       61 aivlgilfvs lsacvcirrr pmpskdaark fapptsngvk gvttadfkea svgsrddlng
      121 sapsarpsdg llnialekge ffhntsvdps nprraqqkpv tssprttkak lvrdgavfpe
      181 ppkvseipvg srsqpdslrr akvkngklpk apasvgdfas lglqddddav fsatekskps
      241 vkgrgkvplt afkqtsksvs dlddessfes strapqqrta vrpetpqmep rlavschnil
      301 seepvrvgia hlrqredpsv srkppvprkv tgialpgmvq rapgagaasl sqddlsrvrl
      361 kpaeqkprap lrdqgpedgk qrlqhksvpd ffpqdggvrl tlldsssssy gkssrttapa
      421 petplntsla syadaagped lhrcntvglp paplpkpats gqqfrvyapk qdslvpdkqp
      481 lmaewaasis sksdtfssde aecqrhlsss qellmgnvkc letei
//
LOCUS       XP_077502914             519 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113624 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077502914
VERSION     XP_077502914.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646788.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..519
                     /product="uncharacterized protein LOC144113624 isoform X4"
                     /calculated_mol_wt=55210
     CDS             1..519
                     /gene="LOC144113624"
                     /coded_by="XM_077646788.1:120..1679"
                     /db_xref="GeneID:144113624"
ORIGIN      
        1 massrppleg gcsrvcvill ylsgmlliia gvvaggfivn ypdilfylav asgsaivlgi
       61 lfvslsacvc irrrpmpskd aarkfappts ngvkgvttad fkeasvgsrd dlngsapsar
      121 psdgllnial ekgeffhnts vdpsnprraq qkpvtssprt tkaklvrdga vfpeppkvse
      181 ipvgsrsqpd slrrakvkng klpkapasvg dfaslglqdd ddavfsatek skpsvkgrgk
      241 vpltafkqts ksvsdlddes sfesstrapq qrtavrpetp qmeprlavsc hnilseepvr
      301 vgiahlrqre dpsvsrkppv prkvtgialp gmvqrapgag aaslsqddls rvrlkpaeqk
      361 praplrdqgp edgkqrlqhk svpdffpqdg gvrltlldss sssygkssrt tapapetpln
      421 tslasyadaa gpedlhrcnt vglppaplpk patsgqqfrv yapkqdslvp dkqplmaewa
      481 asissksdtf ssdeaecqrh lsssqellmg nvkcletei
//
LOCUS       XP_077502915             431 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113626 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502915
VERSION     XP_077502915.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646789.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..431
                     /product="uncharacterized protein LOC144113626 isoform X1"
                     /calculated_mol_wt=46480
     CDS             1..431
                     /gene="LOC144113626"
                     /coded_by="XM_077646789.1:88..1383"
                     /db_xref="GeneID:144113626"
ORIGIN      
        1 mlakpmlssp ptrywtaaal argewagvsr pasmdaghrs lppsrrekkr ppaegplltl
       61 aggapewldv ipcgqlgavr dvfkaaaadd ksavpscyes cdstslssfd ngseggakep
      121 rskdafagry sfksraddrt dggcqitrlc mnrdgsvrst gfvprvtdag eeeppeilvt
      181 advsrcsdde ngsgcaigrg spyaslprdf lrvprsrkgr lvlsgpkkag rkkgsevfev
      241 rrvpsdsads giytsfgkei rsynlpqdsa tpagrnmptp epevaessla tvttpaatta
      301 esppdegaag saqvemerrr rqrntelati fifipvliaw vlivplvlfs ysfenpyskh
      361 fevalwicip lstivlsatc lvsnvywyyd reagrwryli hcgkgpagfy wgdvltepgn
      421 gkqqtggvev v
//
LOCUS       XP_077502916             430 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113626 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502916
VERSION     XP_077502916.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646790.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..430
                     /product="uncharacterized protein LOC144113626 isoform X2"
                     /calculated_mol_wt=46409
     CDS             1..430
                     /gene="LOC144113626"
                     /coded_by="XM_077646790.1:114..1406"
                     /db_xref="GeneID:144113626"
ORIGIN      
        1 mlakpmlssp ptrywtaala rgewagvsrp asmdaghrsl ppsrrekkrp paegplltla
       61 ggapewldvi pcgqlgavrd vfkaaaaddk savpscyesc dstslssfdn gseggakepr
      121 skdafagrys fksraddrtd ggcqitrlcm nrdgsvrstg fvprvtdage eeppeilvta
      181 dvsrcsdden gsgcaigrgs pyaslprdfl rvprsrkgrl vlsgpkkagr kkgsevfevr
      241 rvpsdsadsg iytsfgkeir synlpqdsat pagrnmptpe pevaesslat vttpaattae
      301 sppdegaags aqvemerrrr qrntelatif ifipvliawv livplvlfsy sfenpyskhf
      361 evalwicipl stivlsatcl vsnvywyydr eagrwrylih cgkgpagfyw gdvltepgng
      421 kqqtggvevv
//
LOCUS       XP_077502917             398 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113626 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502917
VERSION     XP_077502917.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646791.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..398
                     /product="uncharacterized protein LOC144113626 isoform X3"
                     /calculated_mol_wt=42966
     CDS             1..398
                     /gene="LOC144113626"
                     /coded_by="XM_077646791.1:222..1418"
                     /db_xref="GeneID:144113626"
ORIGIN      
        1 mdaghrslpp srrekkrppa egplltlagg apewldvipc gqlgavrdvf kaaaaddksa
       61 vpscyescds tslssfdngs eggakeprsk dafagrysfk sraddrtdgg cqitrlcmnr
      121 dgsvrstgfv prvtdageee ppeilvtadv srcsddengs gcaigrgspy aslprdflrv
      181 prsrkgrlvl sgpkkagrkk gsevfevrrv psdsadsgiy tsfgkeirsy nlpqdsatpa
      241 grnmptpepe vaesslatvt tpaattaesp pdegaagsaq vemerrrrqr ntelatifif
      301 ipvliawvli vplvlfsysf enpyskhfev alwiciplst ivlsatclvs nvywyydrea
      361 grwrylihcg kgpagfywgd vltepgngkq qtggvevv
//
LOCUS       XP_077502918             398 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113626 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502918
VERSION     XP_077502918.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646792.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..398
                     /product="uncharacterized protein LOC144113626 isoform X3"
                     /calculated_mol_wt=42966
     CDS             1..398
                     /gene="LOC144113626"
                     /coded_by="XM_077646792.1:216..1412"
                     /db_xref="GeneID:144113626"
ORIGIN      
        1 mdaghrslpp srrekkrppa egplltlagg apewldvipc gqlgavrdvf kaaaaddksa
       61 vpscyescds tslssfdngs eggakeprsk dafagrysfk sraddrtdgg cqitrlcmnr
      121 dgsvrstgfv prvtdageee ppeilvtadv srcsddengs gcaigrgspy aslprdflrv
      181 prsrkgrlvl sgpkkagrkk gsevfevrrv psdsadsgiy tsfgkeirsy nlpqdsatpa
      241 grnmptpepe vaesslatvt tpaattaesp pdegaagsaq vemerrrrqr ntelatifif
      301 ipvliawvli vplvlfsysf enpyskhfev alwiciplst ivlsatclvs nvywyydrea
      361 grwrylihcg kgpagfywgd vltepgngkq qtggvevv
//
LOCUS       XP_077502919             398 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113626 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502919
VERSION     XP_077502919.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646793.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..398
                     /product="uncharacterized protein LOC144113626 isoform X3"
                     /calculated_mol_wt=42966
     CDS             1..398
                     /gene="LOC144113626"
                     /coded_by="XM_077646793.1:169..1365"
                     /db_xref="GeneID:144113626"
ORIGIN      
        1 mdaghrslpp srrekkrppa egplltlagg apewldvipc gqlgavrdvf kaaaaddksa
       61 vpscyescds tslssfdngs eggakeprsk dafagrysfk sraddrtdgg cqitrlcmnr
      121 dgsvrstgfv prvtdageee ppeilvtadv srcsddengs gcaigrgspy aslprdflrv
      181 prsrkgrlvl sgpkkagrkk gsevfevrrv psdsadsgiy tsfgkeirsy nlpqdsatpa
      241 grnmptpepe vaesslatvt tpaattaesp pdegaagsaq vemerrrrqr ntelatifif
      301 ipvliawvli vplvlfsysf enpyskhfev alwiciplst ivlsatclvs nvywyydrea
      361 grwrylihcg kgpagfywgd vltepgngkq qtggvevv
//
LOCUS       XP_077502920             398 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113626 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502920
VERSION     XP_077502920.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646794.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..398
                     /product="uncharacterized protein LOC144113626 isoform X3"
                     /calculated_mol_wt=42966
     CDS             1..398
                     /gene="LOC144113626"
                     /coded_by="XM_077646794.1:219..1415"
                     /db_xref="GeneID:144113626"
ORIGIN      
        1 mdaghrslpp srrekkrppa egplltlagg apewldvipc gqlgavrdvf kaaaaddksa
       61 vpscyescds tslssfdngs eggakeprsk dafagrysfk sraddrtdgg cqitrlcmnr
      121 dgsvrstgfv prvtdageee ppeilvtadv srcsddengs gcaigrgspy aslprdflrv
      181 prsrkgrlvl sgpkkagrkk gsevfevrrv psdsadsgiy tsfgkeirsy nlpqdsatpa
      241 grnmptpepe vaesslatvt tpaattaesp pdegaagsaq vemerrrrqr ntelatifif
      301 ipvliawvli vplvlfsysf enpyskhfev alwiciplst ivlsatclvs nvywyydrea
      361 grwrylihcg kgpagfywgd vltepgngkq qtggvevv
//
LOCUS       XP_077502921             224 aa            linear   INV 25-NOV-2025
DEFINITION  CD63 antigen-like [Amblyomma americanum].
ACCESSION   XP_077502921
VERSION     XP_077502921.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646795.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..224
                     /product="CD63 antigen-like"
                     /calculated_mol_wt=24346
     Region          10..216
                     /region_name="Tetraspanin"
                     /note="Tetraspanin family; pfam00335"
                     /db_xref="CDD:459767"
     CDS             1..224
                     /gene="LOC144113627"
                     /coded_by="XM_077646795.1:126..800"
                     /db_xref="GeneID:144113627"
ORIGIN      
        1 mvnsskmgfv kyllfafnai fvlcgialia vgavvlsaln pqkpfyegyl gapvaivvlg
       61 vfvfvlaflg ccgairenyh mimafsvllt iilicelgaa iaayayrski ahaireeatk
      121 sihnyhnntd vqrlwddiqs slhccganst adydvlppsc cpekpqdctp akayerncvd
      181 vlldkfneki vyvgvtgiii cfievvgivf gcclakairk yevv
//
LOCUS       XP_077502922             668 aa            linear   INV 25-NOV-2025
DEFINITION  hormone receptor-like in 38 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502922
VERSION     XP_077502922.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646796.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..668
                     /product="hormone receptor-like in 38 isoform X1"
                     /calculated_mol_wt=72653
     Region          333..407
                     /region_name="NR_DBD_NGFI-B"
                     /note="DNA-binding domain of the orphan nuclear receptor,
                     nerve growth factor-induced-B; cd06969"
                     /db_xref="CDD:143527"
     Site            order(335,338,352,355,371,377,387,390)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143527"
     Site            order(344..347,353..354,356,358,360..361,384..385,388,391,
                     404..406)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143527"
     Region          435..668
                     /region_name="NR_LBD"
                     /note="The ligand binding domain of nuclear receptors, a
                     family of ligand-activated transcription regulators;
                     cl11397"
                     /db_xref="CDD:472173"
     Site            order(476..477,480,483..484,513..514,517..518,521,524,556)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132757"
     Site            order(485,488,492,497,502..503,505..506,509..510)
                     /site_type="active"
                     /note="coregulator recognition site [active]"
                     /db_xref="CDD:132757"
     CDS             1..668
                     /gene="Hr38"
                     /coded_by="XM_077646796.1:114..2120"
                     /db_xref="GeneID:144113628"
ORIGIN      
        1 meldaalsiq gawhglvrra hsaalsstss llhadylsss ftaeeslsls eelklpgldl
       61 vgaqvpsevf gstvrevssy qemfqsgfpv vsspplteqp tpltplsisp anpsalpsfe
      121 etyspryrrd ithsgvfsfk feelrtdael paeagspeay qvsvggfspk phtstayrpp
      181 ftppppppam agssqefykt eppsyqsssc yvqyqaeqeh akagfsgylh psppvfqkgl
      241 vfpgpsesss fslvsagsss sltsfpgdlt pplpspggsw pqarkppllk asaaattase
      301 tggrlappsa pgtppasgss tasspppatp syqlcavcgd naacqhygvr tcegckgffk
      361 rtvqkgakyv clanrdcpvd krrrnrcqfc rfqkclavgm vkevvrtdsl kgrrgrlpsk
      421 pkspqespps ppvslitalv rahvdtspdl anrdyslcqe mpvdesrrta aevqqfynll
      481 tssvdviktf sekipgfsel dksdqellfq saslelfalr layrirqedd eftfcngvvl
      541 hrtqcerifg ewlaaifefs rslhamqidi safaclaalt lvterhglkd gskverlqmk
      601 iigslrdhvt ynseaqkkph yfsrllaklp dlrslsvqgl qrifylkled lvpaptvien
      661 mfvsslpf
//
LOCUS       XP_077502923             666 aa            linear   INV 25-NOV-2025
DEFINITION  hormone receptor-like in 38 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502923
VERSION     XP_077502923.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646797.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..666
                     /product="hormone receptor-like in 38 isoform X2"
                     /calculated_mol_wt=72333
     Region          331..405
                     /region_name="NR_DBD_NGFI-B"
                     /note="DNA-binding domain of the orphan nuclear receptor,
                     nerve growth factor-induced-B; cd06969"
                     /db_xref="CDD:143527"
     Site            order(333,336,350,353,369,375,385,388)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143527"
     Site            order(342..345,351..352,354,356,358..359,382..383,386,389,
                     402..404)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143527"
     Region          433..666
                     /region_name="NR_LBD"
                     /note="The ligand binding domain of nuclear receptors, a
                     family of ligand-activated transcription regulators;
                     cl11397"
                     /db_xref="CDD:472173"
     Site            order(474..475,478,481..482,511..512,515..516,519,522,554)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132757"
     Site            order(483,486,490,495,500..501,503..504,507..508)
                     /site_type="active"
                     /note="coregulator recognition site [active]"
                     /db_xref="CDD:132757"
     CDS             1..666
                     /gene="Hr38"
                     /coded_by="XM_077646797.1:114..2114"
                     /db_xref="GeneID:144113628"
ORIGIN      
        1 meldaalsiq gawhglvrra hsaalsstss llhadylsss ftaeeslsls eelklpgldl
       61 vgaqvpsevf gstvrevssy qemfqsgfpv vsspplteqp tpltplsisp anpsalpsfe
      121 etysprrdit hsgvfsfkfe elrtdaelpa eagspeayqv svggfspkph tstayrppft
      181 ppppppamag ssqefyktep psyqssscyv qyqaeqehak agfsgylhps ppvfqkglvf
      241 pgpsesssfs lvsagssssl tsfpgdltpp lpspggswpq arkppllkas aaattasetg
      301 grlappsapg tppasgssta sspppatpsy qlcavcgdna acqhygvrtc egckgffkrt
      361 vqkgakyvcl anrdcpvdkr rrnrcqfcrf qkclavgmvk evvrtdslkg rrgrlpskpk
      421 spqesppspp vslitalvra hvdtspdlan rdyslcqemp vdesrrtaae vqqfynllts
      481 svdviktfse kipgfseldk sdqellfqsa slelfalrla yrirqeddef tfcngvvlhr
      541 tqcerifgew laaifefsrs lhamqidisa faclaaltlv terhglkdgs kverlqmkii
      601 gslrdhvtyn seaqkkphyf srllaklpdl rslsvqglqr ifylkledlv paptvienmf
      661 vsslpf
//
LOCUS       XP_077502924             666 aa            linear   INV 25-NOV-2025
DEFINITION  hormone receptor-like in 38 isoform X3 [Amblyomma americanum].
ACCESSION   XP_077502924
VERSION     XP_077502924.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646798.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..666
                     /product="hormone receptor-like in 38 isoform X3"
                     /calculated_mol_wt=72519
     Region          331..405
                     /region_name="NR_DBD_NGFI-B"
                     /note="DNA-binding domain of the orphan nuclear receptor,
                     nerve growth factor-induced-B; cd06969"
                     /db_xref="CDD:143527"
     Site            order(333,336,350,353,369,375,385,388)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143527"
     Site            order(342..345,351..352,354,356,358..359,382..383,386,389,
                     402..404)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143527"
     Region          433..666
                     /region_name="NR_LBD"
                     /note="The ligand binding domain of nuclear receptors, a
                     family of ligand-activated transcription regulators;
                     cl11397"
                     /db_xref="CDD:472173"
     Site            order(474..475,478,481..482,511..512,515..516,519,522,554)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132757"
     Site            order(483,486,490,495,500..501,503..504,507..508)
                     /site_type="active"
                     /note="coregulator recognition site [active]"
                     /db_xref="CDD:132757"
     CDS             1..666
                     /gene="Hr38"
                     /coded_by="XM_077646798.1:115..2115"
                     /db_xref="GeneID:144113628"
ORIGIN      
        1 meldaalsiq gawhglvrra hvrlqghqpr vagpaaatyp lghwprgpaa salarsstcd
       61 sifcfprale naarsqvrhr vemfqsgfpv vsspplteqp tpltplsisp anpsalpsfe
      121 etysprrdit hsgvfsfkfe elrtdaelpa eagspeayqv svggfspkph tstayrppft
      181 ppppppamag ssqefyktep psyqssscyv qyqaeqehak agfsgylhps ppvfqkglvf
      241 pgpsesssfs lvsagssssl tsfpgdltpp lpspggswpq arkppllkas aaattasetg
      301 grlappsapg tppasgssta sspppatpsy qlcavcgdna acqhygvrtc egckgffkrt
      361 vqkgakyvcl anrdcpvdkr rrnrcqfcrf qkclavgmvk evvrtdslkg rrgrlpskpk
      421 spqesppspp vslitalvra hvdtspdlan rdyslcqemp vdesrrtaae vqqfynllts
      481 svdviktfse kipgfseldk sdqellfqsa slelfalrla yrirqeddef tfcngvvlhr
      541 tqcerifgew laaifefsrs lhamqidisa faclaaltlv terhglkdgs kverlqmkii
      601 gslrdhvtyn seaqkkphyf srllaklpdl rslsvqglqr ifylkledlv paptvienmf
      661 vsslpf
//
LOCUS       XP_077502925             661 aa            linear   INV 25-NOV-2025
DEFINITION  hormone receptor-like in 38 isoform X4 [Amblyomma americanum].
ACCESSION   XP_077502925
VERSION     XP_077502925.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646799.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..661
                     /product="hormone receptor-like in 38 isoform X4"
                     /calculated_mol_wt=71871
     Region          326..400
                     /region_name="NR_DBD_NGFI-B"
                     /note="DNA-binding domain of the orphan nuclear receptor,
                     nerve growth factor-induced-B; cd06969"
                     /db_xref="CDD:143527"
     Site            order(328,331,345,348,364,370,380,383)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143527"
     Site            order(337..340,346..347,349,351,353..354,377..378,381,384,
                     397..399)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143527"
     Region          428..661
                     /region_name="NR_LBD"
                     /note="The ligand binding domain of nuclear receptors, a
                     family of ligand-activated transcription regulators;
                     cl11397"
                     /db_xref="CDD:472173"
     Site            order(469..470,473,476..477,506..507,510..511,514,517,549)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132757"
     Site            order(478,481,485,490,495..496,498..499,502..503)
                     /site_type="active"
                     /note="coregulator recognition site [active]"
                     /db_xref="CDD:132757"
     CDS             1..661
                     /gene="Hr38"
                     /coded_by="XM_077646799.1:114..2099"
                     /db_xref="GeneID:144113628"
ORIGIN      
        1 meldaalsiq gawhglvrra hvrlqghqpr vagpaaatyp lghwprgpaa salarsstcd
       61 sifcfprale naarsqemfq sgfpvvsspp lteqptpltp lsispanpsa lpsfeetysp
      121 rrdithsgvf sfkfeelrtd aelpaeagsp eayqvsvggf spkphtstay rppftppppp
      181 pamagssqef ykteppsyqs sscyvqyqae qehakagfsg ylhpsppvfq kglvfpgpse
      241 sssfslvsag ssssltsfpg dltpplpspg gswpqarkpp llkasaaatt asetggrlap
      301 psapgtppas gsstassppp atpsyqlcav cgdnaacqhy gvrtcegckg ffkrtvqkga
      361 kyvclanrdc pvdkrrrnrc qfcrfqkcla vgmvkevvrt dslkgrrgrl pskpkspqes
      421 ppsppvslit alvrahvdts pdlanrdysl cqempvdesr rtaaevqqfy nlltssvdvi
      481 ktfsekipgf seldksdqel lfqsaslelf alrlayrirq eddeftfcng vvlhrtqcer
      541 ifgewlaaif efsrslhamq idisafacla altlvterhg lkdgskverl qmkiigslrd
      601 hvtynseaqk kphyfsrlla klpdlrslsv qglqrifylk ledlvpaptv ienmfvsslp
      661 f
//
LOCUS       XP_077502926             653 aa            linear   INV 25-NOV-2025
DEFINITION  hormone receptor-like in 38 isoform X5 [Amblyomma americanum].
ACCESSION   XP_077502926
VERSION     XP_077502926.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646800.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..653
                     /product="hormone receptor-like in 38 isoform X5"
                     /calculated_mol_wt=70932
     Region          318..392
                     /region_name="NR_DBD_NGFI-B"
                     /note="DNA-binding domain of the orphan nuclear receptor,
                     nerve growth factor-induced-B; cd06969"
                     /db_xref="CDD:143527"
     Site            order(320,323,337,340,356,362,372,375)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143527"
     Site            order(329..332,338..339,341,343,345..346,369..370,373,376,
                     389..391)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143527"
     Region          420..653
                     /region_name="NR_LBD"
                     /note="The ligand binding domain of nuclear receptors, a
                     family of ligand-activated transcription regulators;
                     cl11397"
                     /db_xref="CDD:472173"
     Site            order(461..462,465,468..469,498..499,502..503,506,509,541)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132757"
     Site            order(470,473,477,482,487..488,490..491,494..495)
                     /site_type="active"
                     /note="coregulator recognition site [active]"
                     /db_xref="CDD:132757"
     CDS             1..653
                     /gene="Hr38"
                     /coded_by="XM_077646800.1:255..2216"
                     /db_xref="GeneID:144113628"
ORIGIN      
        1 mlllespqsa alsstssllh adylsssfta eeslslseel klpgldlvga qvpsevfgst
       61 vrevssyqem fqsgfpvvss pplteqptpl tplsispanp salpsfeety sprrdithsg
      121 vfsfkfeelr tdaelpaeag speayqvsvg gfspkphtst ayrppftppp pppamagssq
      181 efykteppsy qssscyvqyq aeqehakagf sgylhpsppv fqkglvfpgp sesssfslvs
      241 agssssltsf pgdltpplps pggswpqark ppllkasaaa ttasetggrl appsapgtpp
      301 asgsstassp ppatpsyqlc avcgdnaacq hygvrtcegc kgffkrtvqk gakyvclanr
      361 dcpvdkrrrn rcqfcrfqkc lavgmvkevv rtdslkgrrg rlpskpkspq esppsppvsl
      421 italvrahvd tspdlanrdy slcqempvde srrtaaevqq fynlltssvd viktfsekip
      481 gfseldksdq ellfqsasle lfalrlayri rqeddeftfc ngvvlhrtqc erifgewlaa
      541 ifefsrslha mqidisafac laaltlvter hglkdgskve rlqmkiigsl rdhvtynsea
      601 qkkphyfsrl laklpdlrsl svqglqrify lkledlvpap tvienmfvss lpf
//
LOCUS       XP_077502927             608 aa            linear   INV 25-NOV-2025
DEFINITION  hormone receptor-like in 38 isoform X6 [Amblyomma americanum].
ACCESSION   XP_077502927
VERSION     XP_077502927.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646801.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..608
                     /product="hormone receptor-like in 38 isoform X6"
                     /calculated_mol_wt=66392
     Region          273..347
                     /region_name="NR_DBD_NGFI-B"
                     /note="DNA-binding domain of the orphan nuclear receptor,
                     nerve growth factor-induced-B; cd06969"
                     /db_xref="CDD:143527"
     Site            order(275,278,292,295,311,317,327,330)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143527"
     Site            order(284..287,293..294,296,298,300..301,324..325,328,331,
                     344..346)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143527"
     Region          375..608
                     /region_name="NR_LBD"
                     /note="The ligand binding domain of nuclear receptors, a
                     family of ligand-activated transcription regulators;
                     cl11397"
                     /db_xref="CDD:472173"
     Site            order(416..417,420,423..424,453..454,457..458,461,464,496)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132757"
     Site            order(425,428,432,437,442..443,445..446,449..450)
                     /site_type="active"
                     /note="coregulator recognition site [active]"
                     /db_xref="CDD:132757"
     CDS             1..608
                     /gene="Hr38"
                     /coded_by="XM_077646801.1:113..1939"
                     /db_xref="GeneID:144113628"
ORIGIN      
        1 meldaalsiq gawhglvrra hemfqsgfpv vsspplteqp tpltplsisp anpsalpsfe
       61 etyspryrrd ithsgvfsfk feelrtdael paeagspeay qvsvggfspk phtstayrpp
      121 ftppppppam agssqefykt eppsyqsssc yvqyqaeqeh akagfsgylh psppvfqkgl
      181 vfpgpsesss fslvsagsss sltsfpgdlt pplpspggsw pqarkppllk asaaattase
      241 tggrlappsa pgtppasgss tasspppatp syqlcavcgd naacqhygvr tcegckgffk
      301 rtvqkgakyv clanrdcpvd krrrnrcqfc rfqkclavgm vkevvrtdsl kgrrgrlpsk
      361 pkspqespps ppvslitalv rahvdtspdl anrdyslcqe mpvdesrrta aevqqfynll
      421 tssvdviktf sekipgfsel dksdqellfq saslelfalr layrirqedd eftfcngvvl
      481 hrtqcerifg ewlaaifefs rslhamqidi safaclaalt lvterhglkd gskverlqmk
      541 iigslrdhvt ynseaqkkph yfsrllaklp dlrslsvqgl qrifylkled lvpaptvien
      601 mfvsslpf
//
LOCUS       XP_077502928             606 aa            linear   INV 25-NOV-2025
DEFINITION  hormone receptor-like in 38 isoform X7 [Amblyomma americanum].
ACCESSION   XP_077502928
VERSION     XP_077502928.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646802.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..606
                     /product="hormone receptor-like in 38 isoform X7"
                     /calculated_mol_wt=66072
     Region          271..345
                     /region_name="NR_DBD_NGFI-B"
                     /note="DNA-binding domain of the orphan nuclear receptor,
                     nerve growth factor-induced-B; cd06969"
                     /db_xref="CDD:143527"
     Site            order(273,276,290,293,309,315,325,328)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143527"
     Site            order(282..285,291..292,294,296,298..299,322..323,326,329,
                     342..344)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143527"
     Region          373..606
                     /region_name="NR_LBD"
                     /note="The ligand binding domain of nuclear receptors, a
                     family of ligand-activated transcription regulators;
                     cl11397"
                     /db_xref="CDD:472173"
     Site            order(414..415,418,421..422,451..452,455..456,459,462,494)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132757"
     Site            order(423,426,430,435,440..441,443..444,447..448)
                     /site_type="active"
                     /note="coregulator recognition site [active]"
                     /db_xref="CDD:132757"
     CDS             1..606
                     /gene="Hr38"
                     /coded_by="XM_077646802.1:202..2022"
                     /db_xref="GeneID:144113628"
ORIGIN      
        1 meldaalsiq gawhglvrra hemfqsgfpv vsspplteqp tpltplsisp anpsalpsfe
       61 etysprrdit hsgvfsfkfe elrtdaelpa eagspeayqv svggfspkph tstayrppft
      121 ppppppamag ssqefyktep psyqssscyv qyqaeqehak agfsgylhps ppvfqkglvf
      181 pgpsesssfs lvsagssssl tsfpgdltpp lpspggswpq arkppllkas aaattasetg
      241 grlappsapg tppasgssta sspppatpsy qlcavcgdna acqhygvrtc egckgffkrt
      301 vqkgakyvcl anrdcpvdkr rrnrcqfcrf qkclavgmvk evvrtdslkg rrgrlpskpk
      361 spqesppspp vslitalvra hvdtspdlan rdyslcqemp vdesrrtaae vqqfynllts
      421 svdviktfse kipgfseldk sdqellfqsa slelfalrla yrirqeddef tfcngvvlhr
      481 tqcerifgew laaifefsrs lhamqidisa faclaaltlv terhglkdgs kverlqmkii
      541 gslrdhvtyn seaqkkphyf srllaklpdl rslsvqglqr ifylkledlv paptvienmf
      601 vsslpf
//
LOCUS       XP_077502929             595 aa            linear   INV 25-NOV-2025
DEFINITION  hormone receptor-like in 38 isoform X8 [Amblyomma americanum].
ACCESSION   XP_077502929
VERSION     XP_077502929.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646803.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..595
                     /product="hormone receptor-like in 38 isoform X8"
                     /calculated_mol_wt=64990
     Region          260..334
                     /region_name="NR_DBD_NGFI-B"
                     /note="DNA-binding domain of the orphan nuclear receptor,
                     nerve growth factor-induced-B; cd06969"
                     /db_xref="CDD:143527"
     Site            order(262,265,279,282,298,304,314,317)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143527"
     Site            order(271..274,280..281,283,285,287..288,311..312,315,318,
                     331..333)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143527"
     Region          362..595
                     /region_name="NR_LBD"
                     /note="The ligand binding domain of nuclear receptors, a
                     family of ligand-activated transcription regulators;
                     cl11397"
                     /db_xref="CDD:472173"
     Site            order(403..404,407,410..411,440..441,444..445,448,451,483)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132757"
     Site            order(412,415,419,424,429..430,432..433,436..437)
                     /site_type="active"
                     /note="coregulator recognition site [active]"
                     /db_xref="CDD:132757"
     CDS             1..595
                     /gene="Hr38"
                     /coded_by="XM_077646803.1:533..2320"
                     /db_xref="GeneID:144113628"
ORIGIN      
        1 mlllespqem fqsgfpvvss pplteqptpl tplsispanp salpsfeety spryrrdith
       61 sgvfsfkfee lrtdaelpae agspeayqvs vggfspkpht stayrppftp pppppamags
      121 sqefyktepp syqssscyvq yqaeqehaka gfsgylhpsp pvfqkglvfp gpsesssfsl
      181 vsagsssslt sfpgdltppl pspggswpqa rkppllkasa aattasetgg rlappsapgt
      241 ppasgsstas spppatpsyq lcavcgdnaa cqhygvrtce gckgffkrtv qkgakyvcla
      301 nrdcpvdkrr rnrcqfcrfq kclavgmvke vvrtdslkgr rgrlpskpks pqesppsppv
      361 slitalvrah vdtspdlanr dyslcqempv desrrtaaev qqfynlltss vdviktfsek
      421 ipgfseldks dqellfqsas lelfalrlay rirqeddeft fcngvvlhrt qcerifgewl
      481 aaifefsrsl hamqidisaf aclaaltlvt erhglkdgsk verlqmkiig slrdhvtyns
      541 eaqkkphyfs rllaklpdlr slsvqglqri fylkledlvp aptvienmfv sslpf
//
LOCUS       XP_077502930             593 aa            linear   INV 25-NOV-2025
DEFINITION  hormone receptor-like in 38 isoform X9 [Amblyomma americanum].
ACCESSION   XP_077502930
VERSION     XP_077502930.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646804.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..593
                     /product="hormone receptor-like in 38 isoform X9"
                     /calculated_mol_wt=64671
     Region          258..332
                     /region_name="NR_DBD_NGFI-B"
                     /note="DNA-binding domain of the orphan nuclear receptor,
                     nerve growth factor-induced-B; cd06969"
                     /db_xref="CDD:143527"
     Site            order(260,263,277,280,296,302,312,315)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143527"
     Site            order(269..272,278..279,281,283,285..286,309..310,313,316,
                     329..331)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143527"
     Region          360..593
                     /region_name="NR_LBD"
                     /note="The ligand binding domain of nuclear receptors, a
                     family of ligand-activated transcription regulators;
                     cl11397"
                     /db_xref="CDD:472173"
     Site            order(401..402,405,408..409,438..439,442..443,446,449,481)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132757"
     Site            order(410,413,417,422,427..428,430..431,434..435)
                     /site_type="active"
                     /note="coregulator recognition site [active]"
                     /db_xref="CDD:132757"
     CDS             1..593
                     /gene="Hr38"
                     /coded_by="XM_077646804.1:600..2381"
                     /db_xref="GeneID:144113628"
ORIGIN      
        1 mlllespqem fqsgfpvvss pplteqptpl tplsispanp salpsfeety sprrdithsg
       61 vfsfkfeelr tdaelpaeag speayqvsvg gfspkphtst ayrppftppp pppamagssq
      121 efykteppsy qssscyvqyq aeqehakagf sgylhpsppv fqkglvfpgp sesssfslvs
      181 agssssltsf pgdltpplps pggswpqark ppllkasaaa ttasetggrl appsapgtpp
      241 asgsstassp ppatpsyqlc avcgdnaacq hygvrtcegc kgffkrtvqk gakyvclanr
      301 dcpvdkrrrn rcqfcrfqkc lavgmvkevv rtdslkgrrg rlpskpkspq esppsppvsl
      361 italvrahvd tspdlanrdy slcqempvde srrtaaevqq fynlltssvd viktfsekip
      421 gfseldksdq ellfqsasle lfalrlayri rqeddeftfc ngvvlhrtqc erifgewlaa
      481 ifefsrslha mqidisafac laaltlvter hglkdgskve rlqmkiigsl rdhvtynsea
      541 qkkphyfsrl laklpdlrsl svqglqrify lkledlvpap tvienmfvss lpf
//
LOCUS       XP_077502931             593 aa            linear   INV 25-NOV-2025
DEFINITION  hormone receptor-like in 38 isoform X9 [Amblyomma americanum].
ACCESSION   XP_077502931
VERSION     XP_077502931.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646805.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..593
                     /product="hormone receptor-like in 38 isoform X9"
                     /calculated_mol_wt=64671
     Region          258..332
                     /region_name="NR_DBD_NGFI-B"
                     /note="DNA-binding domain of the orphan nuclear receptor,
                     nerve growth factor-induced-B; cd06969"
                     /db_xref="CDD:143527"
     Site            order(260,263,277,280,296,302,312,315)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143527"
     Site            order(269..272,278..279,281,283,285..286,309..310,313,316,
                     329..331)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143527"
     Region          360..593
                     /region_name="NR_LBD"
                     /note="The ligand binding domain of nuclear receptors, a
                     family of ligand-activated transcription regulators;
                     cl11397"
                     /db_xref="CDD:472173"
     Site            order(401..402,405,408..409,438..439,442..443,446,449,481)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132757"
     Site            order(410,413,417,422,427..428,430..431,434..435)
                     /site_type="active"
                     /note="coregulator recognition site [active]"
                     /db_xref="CDD:132757"
     CDS             1..593
                     /gene="Hr38"
                     /coded_by="XM_077646805.1:434..2215"
                     /db_xref="GeneID:144113628"
ORIGIN      
        1 mlllespqem fqsgfpvvss pplteqptpl tplsispanp salpsfeety sprrdithsg
       61 vfsfkfeelr tdaelpaeag speayqvsvg gfspkphtst ayrppftppp pppamagssq
      121 efykteppsy qssscyvqyq aeqehakagf sgylhpsppv fqkglvfpgp sesssfslvs
      181 agssssltsf pgdltpplps pggswpqark ppllkasaaa ttasetggrl appsapgtpp
      241 asgsstassp ppatpsyqlc avcgdnaacq hygvrtcegc kgffkrtvqk gakyvclanr
      301 dcpvdkrrrn rcqfcrfqkc lavgmvkevv rtdslkgrrg rlpskpkspq esppsppvsl
      361 italvrahvd tspdlanrdy slcqempvde srrtaaevqq fynlltssvd viktfsekip
      421 gfseldksdq ellfqsasle lfalrlayri rqeddeftfc ngvvlhrtqc erifgewlaa
      481 ifefsrslha mqidisafac laaltlvter hglkdgskve rlqmkiigsl rdhvtynsea
      541 qkkphyfsrl laklpdlrsl svqglqrify lkledlvpap tvienmfvss lpf
//
LOCUS       XP_077502932             586 aa            linear   INV 25-NOV-2025
DEFINITION  hormone receptor-like in 38 isoform X10 [Amblyomma americanum].
ACCESSION   XP_077502932
VERSION     XP_077502932.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646806.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..586
                     /product="hormone receptor-like in 38 isoform X10"
                     /calculated_mol_wt=63949
     Region          251..325
                     /region_name="NR_DBD_NGFI-B"
                     /note="DNA-binding domain of the orphan nuclear receptor,
                     nerve growth factor-induced-B; cd06969"
                     /db_xref="CDD:143527"
     Site            order(253,256,270,273,289,295,305,308)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143527"
     Site            order(262..265,271..272,274,276,278..279,302..303,306,309,
                     322..324)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143527"
     Region          353..586
                     /region_name="NR_LBD"
                     /note="The ligand binding domain of nuclear receptors, a
                     family of ligand-activated transcription regulators;
                     cl11397"
                     /db_xref="CDD:472173"
     Site            order(394..395,398,401..402,431..432,435..436,439,442,474)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132757"
     Site            order(403,406,410,415,420..421,423..424,427..428)
                     /site_type="active"
                     /note="coregulator recognition site [active]"
                     /db_xref="CDD:132757"
     CDS             1..586
                     /gene="Hr38"
                     /coded_by="XM_077646806.1:147..1907"
                     /db_xref="GeneID:144113628"
ORIGIN      
        1 mfqsgfpvvs spplteqptp ltplsispan psalpsfeet yspryrrdit hsgvfsfkfe
       61 elrtdaelpa eagspeayqv svggfspkph tstayrppft ppppppamag ssqefyktep
      121 psyqssscyv qyqaeqehak agfsgylhps ppvfqkglvf pgpsesssfs lvsagssssl
      181 tsfpgdltpp lpspggswpq arkppllkas aaattasetg grlappsapg tppasgssta
      241 sspppatpsy qlcavcgdna acqhygvrtc egckgffkrt vqkgakyvcl anrdcpvdkr
      301 rrnrcqfcrf qkclavgmvk evvrtdslkg rrgrlpskpk spqesppspp vslitalvra
      361 hvdtspdlan rdyslcqemp vdesrrtaae vqqfynllts svdviktfse kipgfseldk
      421 sdqellfqsa slelfalrla yrirqeddef tfcngvvlhr tqcerifgew laaifefsrs
      481 lhamqidisa faclaaltlv terhglkdgs kverlqmkii gslrdhvtyn seaqkkphyf
      541 srllaklpdl rslsvqglqr ifylkledlv paptvienmf vsslpf
//
LOCUS       XP_077502933             655 aa            linear   INV 25-NOV-2025
DEFINITION  hormone receptor-like in 38 isoform X11 [Amblyomma americanum].
ACCESSION   XP_077502933
VERSION     XP_077502933.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646807.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..655
                     /product="hormone receptor-like in 38 isoform X11"
                     /calculated_mol_wt=71251
     Region          320..394
                     /region_name="NR_DBD_NGFI-B"
                     /note="DNA-binding domain of the orphan nuclear receptor,
                     nerve growth factor-induced-B; cd06969"
                     /db_xref="CDD:143527"
     Site            order(322,325,339,342,358,364,374,377)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143527"
     Site            order(331..334,340..341,343,345,347..348,371..372,375,378,
                     391..393)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143527"
     Region          422..655
                     /region_name="NR_LBD"
                     /note="The ligand binding domain of nuclear receptors, a
                     family of ligand-activated transcription regulators;
                     cl11397"
                     /db_xref="CDD:472173"
     Site            order(463..464,467,470..471,500..501,504..505,508,511,543)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132757"
     Site            order(472,475,479,484,489..490,492..493,496..497)
                     /site_type="active"
                     /note="coregulator recognition site [active]"
                     /db_xref="CDD:132757"
     CDS             1..655
                     /gene="Hr38"
                     /coded_by="XM_077646807.1:271..2238"
                     /db_xref="GeneID:144113628"
ORIGIN      
        1 mlllespqsa alsstssllh adylsssfta eeslslseel klpgldlvga qvpsevfgst
       61 vrevssyqem fqsgfpvvss pplteqptpl tplsispanp salpsfeety spryrrdith
      121 sgvfsfkfee lrtdaelpae agspeayqvs vggfspkpht stayrppftp pppppamags
      181 sqefyktepp syqssscyvq yqaeqehaka gfsgylhpsp pvfqkglvfp gpsesssfsl
      241 vsagsssslt sfpgdltppl pspggswpqa rkppllkasa aattasetgg rlappsapgt
      301 ppasgsstas spppatpsyq lcavcgdnaa cqhygvrtce gckgffkrtv qkgakyvcla
      361 nrdcpvdkrr rnrcqfcrfq kclavgmvke vvrtdslkgr rgrlpskpks pqesppsppv
      421 slitalvrah vdtspdlanr dyslcqempv desrrtaaev qqfynlltss vdviktfsek
      481 ipgfseldks dqellfqsas lelfalrlay rirqeddeft fcngvvlhrt qcerifgewl
      541 aaifefsrsl hamqidisaf aclaaltlvt erhglkdgsk verlqmkiig slrdhvtyns
      601 eaqkkphyfs rllaklpdlr slsvqglqri fylkledlvp aptvienmfv sslpf
//
LOCUS       XP_077502934             338 aa            linear   INV 25-NOV-2025
DEFINITION  syntaxin 17 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502934
VERSION     XP_077502934.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646808.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..338
                     /product="syntaxin 17 isoform X1"
                     /calculated_mol_wt=37063
     Region          188..249
                     /region_name="SNARE_syntaxin17"
                     /note="SNARE motif of syntaxin 17; cd15846"
                     /db_xref="CDD:277199"
     Site            order(192,194..195,197..202,204..206,208..209,211..213,
                     215..216,218..220,222..223,225..230,232..237,239..244,
                     246..248)
                     /site_type="other"
                     /note="heterotetramer interface [polypeptide binding]"
                     /db_xref="CDD:277199"
     Site            222
                     /site_type="other"
                     /note="zero layer"
                     /db_xref="CDD:277199"
     CDS             1..338
                     /gene="Syx17"
                     /coded_by="XM_077646808.1:466..1482"
                     /db_xref="GeneID:144113630"
ORIGIN      
        1 mpsptagsla vcsglvnemv dsdlrdsahr eiaaepsvkf plrrvklvvh ritkvaiphh
       61 ldilskqrsi igkcqklkqw dklhdeqira sltikqlkad lyeletlrsq vdptdlqqfe
      121 hlvrpaseat feqvrsftei clpvgpvidf saqhtclpev vpaaatdtdv pslekitvsd
      181 qafyvehipd ntaafkswns lkkdlhdlhd lvtsfsslvh aqqdkvetie envtradeqv
      241 aysvvhlaqa aklrkamlpv tgaligltvg gpvgllvgmk valgcalgag flgytggkvl
      301 qrrseqdkvp edmelkempk rhvtdetllt tspvkpvi
//
LOCUS       XP_077502935             320 aa            linear   INV 25-NOV-2025
DEFINITION  syntaxin 17 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502935
VERSION     XP_077502935.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646809.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..320
                     /product="syntaxin 17 isoform X2"
                     /calculated_mol_wt=35348
     Region          170..231
                     /region_name="SNARE_syntaxin17"
                     /note="SNARE motif of syntaxin 17; cd15846"
                     /db_xref="CDD:277199"
     Site            order(174,176..177,179..184,186..188,190..191,193..195,
                     197..198,200..202,204..205,207..212,214..219,221..226,
                     228..230)
                     /site_type="other"
                     /note="heterotetramer interface [polypeptide binding]"
                     /db_xref="CDD:277199"
     Site            204
                     /site_type="other"
                     /note="zero layer"
                     /db_xref="CDD:277199"
     CDS             1..320
                     /gene="Syx17"
                     /coded_by="XM_077646809.1:174..1136"
                     /db_xref="GeneID:144113630"
ORIGIN      
        1 mvdsdlrdsa hreiaaepsv kfplrrvklv vhritkvaip hhldilskqr siigkcqklk
       61 qwdklhdeqi rasltikqlk adlyeletlr sqvdptdlqq fehlvrpase atfeqvrsft
      121 eiclpvgpvi dfsaqhtclp evvpaaatdt dvpslekitv sdqafyvehi pdntaafksw
      181 nslkkdlhdl hdlvtsfssl vhaqqdkvet ieenvtrade qvaysvvhla qaaklrkaml
      241 pvtgaliglt vggpvgllvg mkvalgcalg agflgytggk vlqrrseqdk vpedmelkem
      301 pkrhvtdetl lttspvkpvi
//
LOCUS       XP_077502936             298 aa            linear   INV 25-NOV-2025
DEFINITION  syntaxin 17 isoform X3 [Amblyomma americanum].
ACCESSION   XP_077502936
VERSION     XP_077502936.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646810.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..298
                     /product="syntaxin 17 isoform X3"
                     /calculated_mol_wt=32838
     Region          188..249
                     /region_name="SNARE_syntaxin17"
                     /note="SNARE motif of syntaxin 17; cd15846"
                     /db_xref="CDD:277199"
     Site            order(192,194..195,197..202,204..206,208..209,211..213,
                     215..216,218..220,222..223,225..230,232..237,239..244,
                     246..248)
                     /site_type="other"
                     /note="heterotetramer interface [polypeptide binding]"
                     /db_xref="CDD:277199"
     Site            222
                     /site_type="other"
                     /note="zero layer"
                     /db_xref="CDD:277199"
     CDS             1..298
                     /gene="Syx17"
                     /coded_by="XM_077646810.1:469..1365"
                     /db_xref="GeneID:144113630"
ORIGIN      
        1 mpsptagsla vcsglvnemv dsdlrdsahr eiaaepsvkf plrrvklvvh ritkvaiphh
       61 ldilskqrsi igkcqklkqw dklhdeqira sltikqlkad lyeletlrsq vdptdlqqfe
      121 hlvrpaseat feqvrsftei clpvgpvidf saqhtclpev vpaaatdtdv pslekitvsd
      181 qafyvehipd ntaafkswns lkkdlhdlhd lvtsfsslvh aqqdkvetie envtradeqv
      241 aysvvhlaqa aklrkamlpv tgaligltvg gpvgllvtle grcysagqsk trclktwn
//
LOCUS       XP_077502937             194 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin conjugating enzyme 2 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502937
VERSION     XP_077502937.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646811.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..194
                     /product="ubiquitin conjugating enzyme 2 isoform X1"
                     /calculated_mol_wt=21144
     Region          51..191
                     /region_name="UBCc_UBE2E"
                     /note="Ubiquitin-conjugating enzyme E2, catalytic (UBCc)
                     domain of ubiquitin conjugating enzyme E2 E1-E3 and
                     related proteins; cd23793"
                     /db_xref="CDD:467413"
     CDS             1..194
                     /gene="Ubc2"
                     /coded_by="XM_077646811.1:16..600"
                     /db_xref="GeneID:144113631"
ORIGIN      
        1 msgdrtqagp srpassttrg nssvtvehpg gdrkapkstp kmskalstsa kriqkelaei
       61 tldpppncsa gpkgdnlyew vstilgppgs vyeggvffld ihfspdypfk ppkvtfrtri
      121 yhcninsqgv icldilkdnw spaltiskvl lsicslltdc npadplvgsi atqfmqqree
      181 hdriarlwtk ryat
//
LOCUS       XP_077502938             153 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin conjugating enzyme 2 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502938
VERSION     XP_077502938.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646812.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..153
                     /product="ubiquitin conjugating enzyme 2 isoform X2"
                     /calculated_mol_wt=16968
     Region          10..150
                     /region_name="UBCc_UBE2E"
                     /note="Ubiquitin-conjugating enzyme E2, catalytic (UBCc)
                     domain of ubiquitin conjugating enzyme E2 E1-E3 and
                     related proteins; cd23793"
                     /db_xref="CDD:467413"
     CDS             1..153
                     /gene="Ubc2"
                     /coded_by="XM_077646812.1:234..695"
                     /db_xref="GeneID:144113631"
ORIGIN      
        1 mskalstsak riqkelaeit ldpppncsag pkgdnlyewv stilgppgsv yeggvffldi
       61 hfspdypfkp pkvtfrtriy hcninsqgvi cldilkdnws paltiskvll sicslltdcn
      121 padplvgsia tqfmqqreeh driarlwtkr yat
//
LOCUS       XP_077502939             153 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin conjugating enzyme 2 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502939
VERSION     XP_077502939.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646813.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..153
                     /product="ubiquitin conjugating enzyme 2 isoform X2"
                     /calculated_mol_wt=16968
     Region          10..150
                     /region_name="UBCc_UBE2E"
                     /note="Ubiquitin-conjugating enzyme E2, catalytic (UBCc)
                     domain of ubiquitin conjugating enzyme E2 E1-E3 and
                     related proteins; cd23793"
                     /db_xref="CDD:467413"
     CDS             1..153
                     /gene="Ubc2"
                     /coded_by="XM_077646813.1:261..722"
                     /db_xref="GeneID:144113631"
ORIGIN      
        1 mskalstsak riqkelaeit ldpppncsag pkgdnlyewv stilgppgsv yeggvffldi
       61 hfspdypfkp pkvtfrtriy hcninsqgvi cldilkdnws paltiskvll sicslltdcn
      121 padplvgsia tqfmqqreeh driarlwtkr yat
//
LOCUS       XP_077502940             394 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin-like activating enzyme 5 [Amblyomma americanum].
ACCESSION   XP_077502940
VERSION     XP_077502940.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646814.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..394
                     /product="ubiquitin-like activating enzyme 5"
                     /calculated_mol_wt=43523
     Region          44..288
                     /region_name="ThiF_MoeB_HesA_family"
                     /note="ThiF_MoeB_HesA. Family of E1-like enzymes involved
                     in molybdopterin and thiamine biosynthesis family. The
                     common reaction mechanism catalyzed by MoeB and ThiF, like
                     other E1 enzymes, begins with a nucleophilic attack of the
                     C-terminal carboxylate of MoaD...; cd00757"
                     /db_xref="CDD:238386"
     Site            order(72,74,76,96,98,107,119,172,178)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:238386"
     Site            order(76,173..175,179,197..198,204,206,273,277,284,
                     286..287)
                     /site_type="other"
                     /note="substrate interface [chemical binding]"
                     /db_xref="CDD:238386"
     CDS             1..394
                     /gene="Uba5"
                     /coded_by="XM_077646814.1:126..1310"
                     /db_xref="GeneID:144113632"
ORIGIN      
        1 madsacfkel drklreeehs mrdpgftpre kiaqmssevv dsnpysrlma lkrmgivdny
       61 eairtftvav vgvggvgsva aemltrcgig klilfdydkv elanmnrlff qphqaglskv
      121 eaavktlqfi npdvefdiyn ynittvdnfq hfidtirtgs lqggpvdlvl scvdnfearm
      181 aintacneln qvwiesgvse navsghiqlv vpgetacfac applvvasni dektlkregv
      241 caaslpttmg ivagflvqnt lkyllnfgtv shylgynalq dffptmqmkp nsqcddsfcr
      301 qrqkeyqdre askpapelve vqkeevvhed newgiclvse tveeghddec ampglasglq
      361 laytvpkqqk dedtegaaes eqtleelmaq mksl
//
LOCUS       XP_077502941             344 aa            linear   INV 25-NOV-2025
DEFINITION  ribosomal silencing factor RsfS-like protein, 312 [Amblyomma
            americanum].
ACCESSION   XP_077502941
VERSION     XP_077502941.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646815.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..344
                     /product="ribosomal silencing factor RsfS-like protein,
                     312"
                     /calculated_mol_wt=38389
     Region          200..299
                     /region_name="RsfS"
                     /note="Ribosomal silencing factor during starvation;
                     pfam02410"
                     /db_xref="CDD:460550"
     CDS             1..344
                     /gene="RsfS312"
                     /coded_by="XM_077646815.1:285..1319"
                     /db_xref="GeneID:144113633"
ORIGIN      
        1 mgsqmvltal yrlggfgarq tvyraadlhr dqlcqrlasq aalrrhfsar ysslrkddal
       61 dsklessqss kkplmepdde trsilndiwr dfecvppkps paeggnsgss sgpaaapkgd
      121 vpkekatdgp riqsllhsar anakklfade dssiildste eqsallgaql dvdyipqnvk
      181 vdpfegfnlk rgetgvfeve elvdilreek ltdiavitvp kemvyadylv latavsprqa
      241 rgvteflrkl ykrkkdskdp tlfiegeqta dwkvldmgnv vlhvflpear rhydvetlwt
      301 vgsayddlth ikddpvynll qqqiqllqev qpkdlenrsa mqqs
//
LOCUS       XP_077502942             240 aa            linear   INV 25-NOV-2025
DEFINITION  transmembrane protein 41A-like [Amblyomma americanum].
ACCESSION   XP_077502942
VERSION     XP_077502942.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646816.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..240
                     /product="transmembrane protein 41A-like"
                     /calculated_mol_wt=26547
     Region          51..238
                     /region_name="TVP38"
                     /note="Uncharacterized membrane protein YdjX, related to
                     fungal oxalate transporter, TVP38/TMEM64 family [Function
                     unknown]; COG0398"
                     /db_xref="CDD:440167"
     CDS             1..240
                     /gene="LOC144113635"
                     /coded_by="XM_077646816.1:308..1030"
                     /db_xref="GeneID:144113635"
ORIGIN      
        1 mppilllpac vaaatgwlyl lashlpcieh ggnasclkfp sshqelkdla allssykeqh
       61 aayvfvlfss aylfkqtfai pgsvflnvla galfglwksf lltcllsgmg asqcyllskf
      121 cgqkyvtryf pdkvrllqdk veqngdrlly wllflrlfpm tpnwflnias pvvgvplhlf
      181 fmsvfiglmp ynficvqtgc ilselksmnd iltwttmakm aaiasvallp sllvkkakte
//
LOCUS       XP_077502943             240 aa            linear   INV 25-NOV-2025
DEFINITION  transmembrane protein 41A-like [Amblyomma americanum].
ACCESSION   XP_077502943
VERSION     XP_077502943.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646817.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..240
                     /product="transmembrane protein 41A-like"
                     /calculated_mol_wt=26547
     Region          51..238
                     /region_name="TVP38"
                     /note="Uncharacterized membrane protein YdjX, related to
                     fungal oxalate transporter, TVP38/TMEM64 family [Function
                     unknown]; COG0398"
                     /db_xref="CDD:440167"
     CDS             1..240
                     /gene="LOC144113635"
                     /coded_by="XM_077646817.1:357..1079"
                     /db_xref="GeneID:144113635"
ORIGIN      
        1 mppilllpac vaaatgwlyl lashlpcieh ggnasclkfp sshqelkdla allssykeqh
       61 aayvfvlfss aylfkqtfai pgsvflnvla galfglwksf lltcllsgmg asqcyllskf
      121 cgqkyvtryf pdkvrllqdk veqngdrlly wllflrlfpm tpnwflnias pvvgvplhlf
      181 fmsvfiglmp ynficvqtgc ilselksmnd iltwttmakm aaiasvallp sllvkkakte
//
LOCUS       XP_077502944             140 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113636 [Amblyomma americanum].
ACCESSION   XP_077502944
VERSION     XP_077502944.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646818.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..140
                     /product="uncharacterized protein LOC144113636"
                     /calculated_mol_wt=15953
     CDS             1..140
                     /gene="LOC144113636"
                     /coded_by="XM_077646818.1:135..557"
                     /db_xref="GeneID:144113636"
ORIGIN      
        1 mrtgtlqqdq elrsrrlknd sqrskfssdg tvvdrslnlq givtmlkedt cnksvgginc
       61 sicgklyksk vcftkhiweh smywdlfddm khqdrvlsiq galilysllh pgdlagllvt
      121 dpprlrrrer leragvrthp
//
LOCUS       XP_077502946             789 aa            linear   INV 25-NOV-2025
DEFINITION  dnaJ homolog subfamily C member 7-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502946
VERSION     XP_077502946.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646820.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..789
                     /product="dnaJ homolog subfamily C member 7-like isoform
                     X1"
                     /calculated_mol_wt=89436
     Region          32..224
                     /region_name="VIP2"
                     /note="A family of actin-ADP-ribosylating toxin. A member
                     of the Bacillus-prodiced vegetative insecticidal proteins
                     (VIPs) possesses high specificity against the major insect
                     pest, corn rootworms, and belongs to a classs of binary
                     toxins and regulators of...; cl00173"
                     /db_xref="CDD:469640"
     Region          <290..402
                     /region_name="TadD"
                     /note="Flp pilus assembly protein TadD, contains TPR
                     repeats [Intracellular trafficking, secretion, and
                     vesicular transport, Extracellular structures]; COG5010"
                     /db_xref="CDD:444034"
     Region          304..332
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(305,308..309,312..313,315,339,342..343,346..347,
                     349..350,373,376..377,380..381,384)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          337..367
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          348..642
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Region          372..396
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          429..454
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(465,468..469,471,495,498..499,502..503,505..506,529,
                     534..535,538..539)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          465..488
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          493..523
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(544..545,548..549,551,579,582..583,586..587,
                     589..590,613,616..617,620..621,624)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          544..568
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          573..607
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          612..636
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          665..732
                     /region_name="DnaJ"
                     /note="DnaJ domain; pfam00226"
                     /db_xref="CDD:395170"
     Site            order(693..695,709,712..713,716..717)
                     /site_type="other"
                     /note="HSP70 interaction site [polypeptide binding]"
                     /db_xref="CDD:99751"
     CDS             1..789
                     /gene="LOC144113640"
                     /coded_by="XM_077646820.1:16..2385"
                     /db_xref="GeneID:144113640"
ORIGIN      
        1 malfngvgap sglgwglsda devaahqglr yylqkaeqls rvevklllaq dcadniefsa
       61 swqeaqrlyr rqicavtddg gygvtedhcv alvcytlrlp nisrlfnqsc rnardtessw
      121 rafrfkslwc llveafkllp hsdpacgaly rgvkcmmscs vgglarfpqf vsasvrfdqa
      181 akfarrgvlt lasvpaefvr disrysvyph hrevlvwplc sfnvrrvcpg gshllefrea
      241 eplriprlpv pdaaglrhge eegvlcplla rlqilcvlsa pvgsvapdpd tvvpavqlrq
      301 nrlaevkreq gndlysqqky deavrcytea ieldgcnvay vnnrsacymm lgnyraaldd
      361 crlalqkdpr naksllrevk chvamgdlgs akrslqlire ldaqgfaqar lhkteiarll
      421 ktveilehfl geadkayeaq dyekviycmd rvlqhavsct rcevlkaesl allkkltgar
      481 eiadnimrae ptnadavyvr glcfyyedni ekaiqhfqqv lrlapdhpka svaykrarll
      541 kskkdegnea fnggkyeeaf niytsalevd prnnlanskl yfnratvcsk lnklnqtved
      601 cttaislned yvkaymrrak tymdlemyee avrdyecifr kdqtrenkrl ldqaklalkk
      661 skckdyykvl gipkdatvdd ikkayrkral lhhpdrhsna sedmkreqek kfkelgeayn
      721 ilsdpkkrmr ydegrdldem egcssdpnph vfrtfffetp gfsfstnans fqhnsgfggf
      781 pggfnfqfr
//
LOCUS       XP_077502947             727 aa            linear   INV 25-NOV-2025
DEFINITION  dnaJ homolog subfamily C member 7-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502947
VERSION     XP_077502947.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646821.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..727
                     /product="dnaJ homolog subfamily C member 7-like isoform
                     X2"
                     /calculated_mol_wt=83393
     Region          <26..158
                     /region_name="VIP2"
                     /note="A family of actin-ADP-ribosylating toxin. A member
                     of the Bacillus-prodiced vegetative insecticidal proteins
                     (VIPs) possesses high specificity against the major insect
                     pest, corn rootworms, and belongs to a classs of binary
                     toxins and regulators of...; cl00173"
                     /db_xref="CDD:469640"
     Region          <227..>311
                     /region_name="Spy"
                     /note="Predicted O-linked N-acetylglucosamine transferase,
                     SPINDLY family [Posttranslational modification, protein
                     turnover, chaperones]; COG3914"
                     /db_xref="CDD:443119"
     Region          242..270
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(243,246..247,250..251,253,277,280..281,284..285,
                     287..288,311,314..315,318..319,322)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          275..305
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          286..580
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Region          310..334
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          367..392
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(403,406..407,409,433,436..437,440..441,443..444,467,
                     472..473,476..477)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          403..426
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          431..461
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(482..483,486..487,489,517,520..521,524..525,
                     527..528,551,554..555,558..559,562)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          482..506
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          511..545
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          550..574
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          603..670
                     /region_name="DnaJ"
                     /note="DnaJ domain; pfam00226"
                     /db_xref="CDD:395170"
     Site            order(631..633,647,650..651,654..655)
                     /site_type="other"
                     /note="HSP70 interaction site [polypeptide binding]"
                     /db_xref="CDD:99751"
     CDS             1..727
                     /gene="LOC144113640"
                     /coded_by="XM_077646821.1:16..2199"
                     /db_xref="GeneID:144113640"
ORIGIN      
        1 maavdfyqtr dlcsplwnfm erdcylalic ytlrtpnicm rfnaacrkar pnerswhafp
       61 ykglwclliw afrsippcnn ippelyrgva tycgtgrapv pfphfvstsv ladeasrfgd
      121 qrhllsffrv psefvrdisp ysiyphqael liwplcrfmh ltysgsyeqh ysfydavpvw
      181 ygisaawqlv ygaslessac agpgmtglvm yyfpattavv aphaepltld pevedeimtr
      241 laevkreqgn dlysqqkyde avrcyteaie ldgcnvayvn nrsacymmlg nyraalddcr
      301 lalqkdprna ksllrevkch vamgdlgsak rslqlireld aqgfaqarlh kteiarllkt
      361 veilehflge adkayeaqdy ekviycmdrv lqhavsctrc evlkaeslal lkkltgarei
      421 adnimraept nadavyvrgl cfyyedniek aiqhfqqvlr lapdhpkasv aykrarllks
      481 kkdegneafn ggkyeeafni ytsalevdpr nnlansklyf nratvcskln klnqtvedct
      541 taislnedyv kaymrrakty mdlemyeeav rdyecifrkd qtrenkrlld qaklalkksk
      601 ckdyykvlgi pkdatvddik kayrkrallh hpdrhsnase dmkreqekkf kelgeaynil
      661 sdpkkrmryd egrdldemeg cssdpnphvf rtfffetpgf sfstnansfq hnsgfggfpg
      721 gfnfqfr
//
LOCUS       XP_077502948             638 aa            linear   INV 25-NOV-2025
DEFINITION  dnaJ homolog subfamily C member 7-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502948
VERSION     XP_077502948.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646822.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..638
                     /product="dnaJ homolog subfamily C member 7-like isoform
                     X3"
                     /calculated_mol_wt=72222
     Region          111..251
                     /region_name="TadD"
                     /note="Flp pilus assembly protein TadD, contains TPR
                     repeats [Intracellular trafficking, secretion, and
                     vesicular transport, Extracellular structures]; COG5010"
                     /db_xref="CDD:444034"
     Region          153..181
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(154,157..158,161..162,164,188,191..192,195..196,
                     198..199,222,225..226,229..230,233)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          186..216
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          197..491
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Region          221..245
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          278..303
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(314,317..318,320,344,347..348,351..352,354..355,378,
                     383..384,387..388)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          314..337
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          342..372
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(393..394,397..398,400,428,431..432,435..436,
                     438..439,462,465..466,469..470,473)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          393..417
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          422..456
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          461..485
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          514..581
                     /region_name="DnaJ"
                     /note="DnaJ domain; pfam00226"
                     /db_xref="CDD:395170"
     Site            order(542..544,558,561..562,565..566)
                     /site_type="other"
                     /note="HSP70 interaction site [polypeptide binding]"
                     /db_xref="CDD:99751"
     CDS             1..638
                     /gene="LOC144113640"
                     /coded_by="XM_077646822.1:135..2051"
                     /db_xref="GeneID:144113640"
ORIGIN      
        1 maeprrarap rprppadgmp ilrrnqrgrv viatllvdgv graavrcasv dallrrpegs
       61 rgasparcrs qerlpprgmr cccwaggrsl alqpalstta psicrsvrrc laagrrrkgd
      121 ddgraaarga srrsrtsllt taarpepsns rlaevkreqg ndlysqqkyd eavrcyteai
      181 eldgcnvayv nnrsacymml gnyraalddc rlalqkdprn aksllrevkc hvamgdlgsa
      241 krslqlirel daqgfaqarl hkteiarllk tveilehflg eadkayeaqd yekviycmdr
      301 vlqhavsctr cevlkaesla llkkltgare iadnimraep tnadavyvrg lcfyyednie
      361 kaiqhfqqvl rlapdhpkas vaykrarllk skkdegneaf nggkyeeafn iytsalevdp
      421 rnnlanskly fnratvcskl nklnqtvedc ttaislnedy vkaymrrakt ymdlemyeea
      481 vrdyecifrk dqtrenkrll dqaklalkks kckdyykvlg ipkdatvddi kkayrkrall
      541 hhpdrhsnas edmkreqekk fkelgeayni lsdpkkrmry degrdldeme gcssdpnphv
      601 frtfffetpg fsfstnansf qhnsgfggfp ggfnfqfr
//
LOCUS       XP_077502949             576 aa            linear   INV 25-NOV-2025
DEFINITION  dnaJ homolog subfamily C member 7-like isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077502949
VERSION     XP_077502949.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646823.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..576
                     /product="dnaJ homolog subfamily C member 7-like isoform
                     X4"
                     /calculated_mol_wt=65717
     Region          <89..>160
                     /region_name="Spy"
                     /note="Predicted O-linked N-acetylglucosamine transferase,
                     SPINDLY family [Posttranslational modification, protein
                     turnover, chaperones]; COG3914"
                     /db_xref="CDD:443119"
     Region          91..119
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(92,95..96,99..100,102,126,129..130,133..134,
                     136..137,160,163..164,167..168,171)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          124..154
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          135..429
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Region          159..183
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          216..241
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(252,255..256,258,282,285..286,289..290,292..293,316,
                     321..322,325..326)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          252..275
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          280..310
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(331..332,335..336,338,366,369..370,373..374,
                     376..377,400,403..404,407..408,411)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          331..355
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          360..394
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          399..423
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          452..519
                     /region_name="DnaJ"
                     /note="DnaJ domain; pfam00226"
                     /db_xref="CDD:395170"
     Site            order(480..482,496,499..500,503..504)
                     /site_type="other"
                     /note="HSP70 interaction site [polypeptide binding]"
                     /db_xref="CDD:99751"
     CDS             1..576
                     /gene="LOC144113640"
                     /coded_by="XM_077646823.1:186..1916"
                     /db_xref="GeneID:144113640"
ORIGIN      
        1 maeprrarap rprppadgmp ilrrnqrgrv viatllvdgv graavrcasv dallrrpegs
       61 rgasparcrs qerlpprgmr cccwaggrrl aevkreqgnd lysqqkydea vrcyteaiel
      121 dgcnvayvnn rsacymmlgn yraalddcrl alqkdprnak sllrevkchv amgdlgsakr
      181 slqlirelda qgfaqarlhk teiarllktv eilehflgea dkayeaqdye kviycmdrvl
      241 qhavsctrce vlkaeslall kkltgareia dnimraeptn adavyvrglc fyyednieka
      301 iqhfqqvlrl apdhpkasva ykrarllksk kdegneafng gkyeeafniy tsalevdprn
      361 nlansklyfn ratvcsklnk lnqtvedctt aislnedyvk aymrraktym dlemyeeavr
      421 dyecifrkdq trenkrlldq aklalkkskc kdyykvlgip kdatvddikk ayrkrallhh
      481 pdrhsnased mkreqekkfk elgeaynils dpkkrmryde grdldemegc ssdpnphvfr
      541 tfffetpgfs fstnansfqh nsgfggfpgg fnfqfr
//
LOCUS       XP_077502950             575 aa            linear   INV 25-NOV-2025
DEFINITION  dnaJ homolog subfamily C member 7-like isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077502950
VERSION     XP_077502950.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646824.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..575
                     /product="dnaJ homolog subfamily C member 7-like isoform
                     X5"
                     /calculated_mol_wt=65469
     Region          48..188
                     /region_name="TadD"
                     /note="Flp pilus assembly protein TadD, contains TPR
                     repeats [Intracellular trafficking, secretion, and
                     vesicular transport, Extracellular structures]; COG5010"
                     /db_xref="CDD:444034"
     Region          90..118
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(91,94..95,98..99,101,125,128..129,132..133,135..136,
                     159,162..163,166..167,170)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          123..153
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          134..428
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Region          158..182
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          215..240
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(251,254..255,257,281,284..285,288..289,291..292,315,
                     320..321,324..325)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          251..274
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          279..309
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(330..331,334..335,337,365,368..369,372..373,
                     375..376,399,402..403,406..407,410)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          330..354
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          359..393
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          398..422
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          451..>544
                     /region_name="DnaJ_bact"
                     /note="chaperone protein DnaJ; TIGR02349"
                     /db_xref="CDD:274090"
     CDS             1..575
                     /gene="LOC144113640"
                     /coded_by="XM_077646824.1:331..2058"
                     /db_xref="GeneID:144113640"
ORIGIN      
        1 malqesmdcd vqiieeksdp ppsrqnlalq palsttapsi crsvrrclaa grrrkgdddg
       61 raaargasrr srtsllttaa rpepsnsrla evkreqgndl ysqqkydeav rcyteaield
      121 gcnvayvnnr sacymmlgny raalddcrla lqkdprnaks llrevkchva mgdlgsakrs
      181 lqlireldaq gfaqarlhkt eiarllktve ilehflgead kayeaqdyek viycmdrvlq
      241 havsctrcev lkaeslallk kltgareiad nimraeptna davyvrglcf yyedniekai
      301 qhfqqvlrla pdhpkasvay krarllkskk degneafngg kyeeafniyt salevdprnn
      361 lansklyfnr atvcsklnkl nqtvedctta islnedyvka ymrraktymd lemyeeavrd
      421 yecifrkdqt renkrlldqa klalkkskck dyykvlgipk datvddikka yrkrallhhp
      481 drhsnasedm kreqekkfke lgeaynilsd pkkrmrydeg rdldemegcs sdpnphvfrt
      541 fffetpgfsf stnansfqhn sgfggfpggf nfqfr
//
LOCUS       XP_077502951             517 aa            linear   INV 25-NOV-2025
DEFINITION  dnaJ homolog subfamily C member 7-like isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077502951
VERSION     XP_077502951.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646825.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..517
                     /product="dnaJ homolog subfamily C member 7-like isoform
                     X6"
                     /calculated_mol_wt=59287
     Region          <30..>101
                     /region_name="Spy"
                     /note="Predicted O-linked N-acetylglucosamine transferase,
                     SPINDLY family [Posttranslational modification, protein
                     turnover, chaperones]; COG3914"
                     /db_xref="CDD:443119"
     Region          32..60
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(33,36..37,40..41,43,67,70..71,74..75,77..78,101,
                     104..105,108..109,112)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          65..95
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          76..370
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Region          100..124
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          155..182
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(193,196..197,199,223,226..227,230..231,233..234,257,
                     262..263,266..267)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          193..216
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          221..251
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(272..273,276..277,279,307,310..311,314..315,
                     317..318,341,344..345,348..349,352)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          272..296
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          301..335
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          340..364
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          393..>486
                     /region_name="DnaJ_bact"
                     /note="chaperone protein DnaJ; TIGR02349"
                     /db_xref="CDD:274090"
     CDS             1..517
                     /gene="LOC144113640"
                     /coded_by="XM_077646825.1:220..1773"
                     /db_xref="GeneID:144113640"
ORIGIN      
        1 mvltsracrp pewhstlaiq kvglagmsrr laevkreqgn dlysqqkyde avrcyteaie
       61 ldgcnvayvn nrsacymmlg nyraalddcr lalqkdprna ksllrevkch vamgdlgsak
      121 rslqlireld aqgfaqarlh kteiarllkt veilehflge adkayeaqdy ekviycmdrv
      181 lqhavsctrc evlkaeslal lkkltgarei adnimraept nadavyvrgl cfyyedniek
      241 aiqhfqqvlr lapdhpkasv aykrarllks kkdegneafn ggkyeeafni ytsalevdpr
      301 nnlansklyf nratvcskln klnqtvedct taislnedyv kaymrrakty mdlemyeeav
      361 rdyecifrkd qtrenkrlld qaklalkksk ckdyykvlgi pkdatvddik kayrkrallh
      421 hpdrhsnase dmkreqekkf kelgeaynil sdpkkrmryd egrdldemeg cssdpnphvf
      481 rtfffetpgf sfstnansfq hnsgfggfpg gfnfqfr
//
LOCUS       XP_077502952             513 aa            linear   INV 25-NOV-2025
DEFINITION  dnaJ homolog subfamily C member 7-like isoform X7 [Amblyomma
            americanum].
ACCESSION   XP_077502952
VERSION     XP_077502952.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646826.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..513
                     /product="dnaJ homolog subfamily C member 7-like isoform
                     X7"
                     /calculated_mol_wt=58909
     Region          3..>97
                     /region_name="Spy"
                     /note="Predicted O-linked N-acetylglucosamine transferase,
                     SPINDLY family [Posttranslational modification, protein
                     turnover, chaperones]; COG3914"
                     /db_xref="CDD:443119"
     Region          28..56
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(29,32..33,36..37,39,63,66..67,70..71,73..74,97,
                     100..101,104..105,108)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          61..91
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          72..366
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Region          96..120
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          153..178
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(189,192..193,195,219,222..223,226..227,229..230,253,
                     258..259,262..263)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          189..212
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          217..247
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(268..269,272..273,275,303,306..307,310..311,
                     313..314,337,340..341,344..345,348)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          268..292
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          297..331
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          336..360
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          389..>482
                     /region_name="DnaJ_bact"
                     /note="chaperone protein DnaJ; TIGR02349"
                     /db_xref="CDD:274090"
     CDS             1..513
                     /gene="LOC144113640"
                     /coded_by="XM_077646826.1:51..1592"
                     /db_xref="GeneID:144113640"
ORIGIN      
        1 malqesmdcd vqiieeksdp ppsrqklaev kreqgndlys qqkydeavrc yteaieldgc
       61 nvayvnnrsa cymmlgnyra alddcrlalq kdprnaksll revkchvamg dlgsakrslq
      121 lireldaqgf aqarlhktei arllktveil ehflgeadka yeaqdyekvi ycmdrvlqha
      181 vsctrcevlk aeslallkkl tgareiadni mraeptnada vyvrglcfyy edniekaiqh
      241 fqqvlrlapd hpkasvaykr arllkskkde gneafnggky eeafniytsa levdprnnla
      301 nsklyfnrat vcsklnklnq tvedcttais lnedyvkaym rraktymdle myeeavrdye
      361 cifrkdqtre nkrlldqakl alkkskckdy ykvlgipkda tvddikkayr krallhhpdr
      421 hsnasedmkr eqekkfkelg eaynilsdpk krmrydegrd ldemegcssd pnphvfrtff
      481 fetpgfsfst nansfqhnsg fggfpggfnf qfr
//
LOCUS       XP_077502953             513 aa            linear   INV 25-NOV-2025
DEFINITION  dnaJ homolog subfamily C member 7-like isoform X7 [Amblyomma
            americanum].
ACCESSION   XP_077502953
VERSION     XP_077502953.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646827.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..513
                     /product="dnaJ homolog subfamily C member 7-like isoform
                     X7"
                     /calculated_mol_wt=58909
     Region          3..>97
                     /region_name="Spy"
                     /note="Predicted O-linked N-acetylglucosamine transferase,
                     SPINDLY family [Posttranslational modification, protein
                     turnover, chaperones]; COG3914"
                     /db_xref="CDD:443119"
     Region          28..56
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(29,32..33,36..37,39,63,66..67,70..71,73..74,97,
                     100..101,104..105,108)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          61..91
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          72..366
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Region          96..120
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          153..178
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(189,192..193,195,219,222..223,226..227,229..230,253,
                     258..259,262..263)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          189..212
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          217..247
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(268..269,272..273,275,303,306..307,310..311,
                     313..314,337,340..341,344..345,348)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          268..292
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          297..331
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          336..360
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          389..>482
                     /region_name="DnaJ_bact"
                     /note="chaperone protein DnaJ; TIGR02349"
                     /db_xref="CDD:274090"
     CDS             1..513
                     /gene="LOC144113640"
                     /coded_by="XM_077646827.1:320..1861"
                     /db_xref="GeneID:144113640"
ORIGIN      
        1 malqesmdcd vqiieeksdp ppsrqklaev kreqgndlys qqkydeavrc yteaieldgc
       61 nvayvnnrsa cymmlgnyra alddcrlalq kdprnaksll revkchvamg dlgsakrslq
      121 lireldaqgf aqarlhktei arllktveil ehflgeadka yeaqdyekvi ycmdrvlqha
      181 vsctrcevlk aeslallkkl tgareiadni mraeptnada vyvrglcfyy edniekaiqh
      241 fqqvlrlapd hpkasvaykr arllkskkde gneafnggky eeafniytsa levdprnnla
      301 nsklyfnrat vcsklnklnq tvedcttais lnedyvkaym rraktymdle myeeavrdye
      361 cifrkdqtre nkrlldqakl alkkskckdy ykvlgipkda tvddikkayr krallhhpdr
      421 hsnasedmkr eqekkfkelg eaynilsdpk krmrydegrd ldemegcssd pnphvfrtff
      481 fetpgfsfst nansfqhnsg fggfpggfnf qfr
//
LOCUS       XP_077502955             504 aa            linear   INV 25-NOV-2025
DEFINITION  dnaJ homolog subfamily C member 7-like isoform X8 [Amblyomma
            americanum].
ACCESSION   XP_077502955
VERSION     XP_077502955.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646829.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..504
                     /product="dnaJ homolog subfamily C member 7-like isoform
                     X8"
                     /calculated_mol_wt=57608
     Region          <18..>88
                     /region_name="Spy"
                     /note="Predicted O-linked N-acetylglucosamine transferase,
                     SPINDLY family [Posttranslational modification, protein
                     turnover, chaperones]; COG3914"
                     /db_xref="CDD:443119"
     Region          19..47
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(20,23..24,27..28,30,54,57..58,61..62,64..65,88,
                     91..92,95..96,99)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          52..82
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          63..357
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Region          87..111
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          144..169
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(180,183..184,186,210,213..214,217..218,220..221,244,
                     249..250,253..254)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          180..203
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          208..238
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(259..260,263..264,266,294,297..298,301..302,
                     304..305,328,331..332,335..336,339)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          259..283
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          288..322
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          327..351
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          380..>473
                     /region_name="DnaJ_bact"
                     /note="chaperone protein DnaJ; TIGR02349"
                     /db_xref="CDD:274090"
     CDS             1..504
                     /gene="LOC144113640"
                     /coded_by="XM_077646829.1:113..1627"
                     /db_xref="GeneID:144113640"
ORIGIN      
        1 miigvcmrgp gvsaavllae vkreqgndly sqqkydeavr cyteaieldg cnvayvnnrs
       61 acymmlgnyr aalddcrlal qkdprnaksl lrevkchvam gdlgsakrsl qlireldaqg
      121 faqarlhkte iarllktvei lehflgeadk ayeaqdyekv iycmdrvlqh avsctrcevl
      181 kaeslallkk ltgareiadn imraeptnad avyvrglcfy yedniekaiq hfqqvlrlap
      241 dhpkasvayk rarllkskkd egneafnggk yeeafniyts alevdprnnl ansklyfnra
      301 tvcsklnkln qtvedcttai slnedyvkay mrraktymdl emyeeavrdy ecifrkdqtr
      361 enkrlldqak lalkkskckd yykvlgipkd atvddikkay rkrallhhpd rhsnasedmk
      421 reqekkfkel geaynilsdp kkrmrydegr dldemegcss dpnphvfrtf ffetpgfsfs
      481 tnansfqhns gfggfpggfn fqfr
//
LOCUS       XP_077502956             200 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113643 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502956
VERSION     XP_077502956.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646830.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..200
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..200
                     /product="uncharacterized protein LOC144113643 isoform X1"
                     /calculated_mol_wt=22596
     Region          <124..167
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     CDS             1..200
                     /gene="LOC144113643"
                     /coded_by="XM_077646830.1:1572..2174"
                     /db_xref="GeneID:144113643"
ORIGIN      
        1 mpdfgawcvr pqydartgwp gqplrlrllp avrlcrpefl tdahamvtgh ksdyvvnlgn
       61 wyrtaddlvl qyelrlqtlp egwerlriss qaqlkslias apdagignll iapealrpfv
      121 sscpqllhrw glqlmhrefl lfkvgeepll rtseegkell ikamryhlmp ewraamagpr
      181 tpakqlaprd adtharpsea
//
LOCUS       XP_077502957             155 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113643 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502957
VERSION     XP_077502957.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646831.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..155
                     /product="uncharacterized protein LOC144113643 isoform X2"
                     /calculated_mol_wt=17435
     Region          <79..122
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     CDS             1..155
                     /gene="LOC144113643"
                     /coded_by="XM_077646831.1:1644..2111"
                     /db_xref="GeneID:144113643"
ORIGIN      
        1 mvtghksdyv vnlgnwyrta ddlvlqyelr lqtlpegwer lrissqaqlk sliasapdag
       61 ignlliapea lrpfvsscpq llhrwglqlm hrefllfkvg eepllrtsee gkellikamr
      121 yhlmpewraa magprtpakq laprdadtha rpsea
//
LOCUS       XP_077502958             155 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113643 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502958
VERSION     XP_077502958.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646832.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..155
                     /product="uncharacterized protein LOC144113643 isoform X2"
                     /calculated_mol_wt=17435
     Region          <79..122
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     CDS             1..155
                     /gene="LOC144113643"
                     /coded_by="XM_077646832.1:306..773"
                     /db_xref="GeneID:144113643"
ORIGIN      
        1 mvtghksdyv vnlgnwyrta ddlvlqyelr lqtlpegwer lrissqaqlk sliasapdag
       61 ignlliapea lrpfvsscpq llhrwglqlm hrefllfkvg eepllrtsee gkellikamr
      121 yhlmpewraa magprtpakq laprdadtha rpsea
//
LOCUS       XP_077502959             151 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113644 [Amblyomma americanum].
ACCESSION   XP_077502959
VERSION     XP_077502959.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646833.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..151
                     /product="uncharacterized protein LOC144113644"
                     /calculated_mol_wt=16044
     CDS             1..151
                     /gene="LOC144113644"
                     /coded_by="XM_077646833.1:132..587"
                     /db_xref="GeneID:144113644"
ORIGIN      
        1 mmhklgglnn fdgleddili sspsrgngaf qlssslhlpr glhfedhgqy atttaksfvs
       61 rscrpplrhs adcrisvhga edlkmmhelg glghfcgled hilqpgvlpl vatssagdrv
      121 vaaifaawia fdscplstsa aqssstmltq w
//
LOCUS       XP_077502960             365 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113645 [Amblyomma americanum].
ACCESSION   XP_077502960
VERSION     XP_077502960.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646834.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..365
                     /product="uncharacterized protein LOC144113645"
                     /calculated_mol_wt=39995
     Region          174..360
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..365
                     /gene="LOC144113645"
                     /coded_by="XM_077646834.1:141..1238"
                     /db_xref="GeneID:144113645"
ORIGIN      
        1 mdcsqslset sasvrragep aavsgepvsk gyvqsrssqs lllfqedadg asptgstlnq
       61 acshasgfim vqsdhtyagp steasassle pgtagvaatl sasadlspga pfsrsftspi
      121 nerrplqklr atlgqlrnrn krlqglllqr hrmkttvelv dslrehltpa vaafveaqlk
      181 mhnvsrfgrr wcsqnktfal glyfhspkgy rycrkllklp svrslqlwla rvplrvgfyp
      241 evfdliekra asfprqdrac tiifdemhvs kelsynpvsd rfegleeysa aqgpnlanka
      301 lvfmakgict pwkqplgyff adkaapatvl ydllfkchkm lvgaglqpva vvcdqgnqnv
      361 cfrnl
//
LOCUS       XP_077502961             643 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113647 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502961
VERSION     XP_077502961.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646835.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..643
                     /product="uncharacterized protein LOC144113647 isoform X1"
                     /calculated_mol_wt=71416
     Region          208..>550
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..643
                     /gene="LOC144113647"
                     /coded_by="XM_077646835.1:208..2139"
                     /db_xref="GeneID:144113647"
ORIGIN      
        1 msegqdhhkr mpttqvatvg sanaaegqpg avmtssqqrp cysdlldsir kihdklitlt
       61 lklifklqvc enaeasnqvl ggrlndpqqr ivelmadnaq chlnmqrlve ehsnvladne
      121 slvlqhrtek eekdqliaql rmlasklcdl sdlddrsalk liealgsavq qlrdertsln
      181 dreraaktti ealtgerdal maenrvlaek qkdynelqqa kesledhlti ykdvaknitr
      241 evhhqltsam gtlkqviqes tiaegpfeat lqtvereavr lrtqislfyd hwshservts
      301 dmsdelarkk eevqrlsaec armdsvireq maeieglcsg itnfeqlaad sagdlarvqa
      361 eaeslkesla aaqmeaeere eaallllkkd tqiaqlegkl qekreelasl vqekvaavde
      421 serfrevnqn lkeivfslsk qleqeqnrre smvtdavqtd gclvpkadce vqtsgnydel
      481 llklessehe vnllkssihr iccdnaalss mykecrgelt kaseichale dqlkrlaeel
      541 kavntdkeal lvktpvkasp rkekhpgsea kqsstsvssv plkmagsrcr vapeqktvdv
      601 sgeavwmtas giaeavgnvr vrsfrgsavp asskkpklek kgd
//
LOCUS       XP_077502962             643 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113647 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502962
VERSION     XP_077502962.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646836.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..643
                     /product="uncharacterized protein LOC144113647 isoform X1"
                     /calculated_mol_wt=71416
     Region          208..>550
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..643
                     /gene="LOC144113647"
                     /coded_by="XM_077646836.1:142..2073"
                     /db_xref="GeneID:144113647"
ORIGIN      
        1 msegqdhhkr mpttqvatvg sanaaegqpg avmtssqqrp cysdlldsir kihdklitlt
       61 lklifklqvc enaeasnqvl ggrlndpqqr ivelmadnaq chlnmqrlve ehsnvladne
      121 slvlqhrtek eekdqliaql rmlasklcdl sdlddrsalk liealgsavq qlrdertsln
      181 dreraaktti ealtgerdal maenrvlaek qkdynelqqa kesledhlti ykdvaknitr
      241 evhhqltsam gtlkqviqes tiaegpfeat lqtvereavr lrtqislfyd hwshservts
      301 dmsdelarkk eevqrlsaec armdsvireq maeieglcsg itnfeqlaad sagdlarvqa
      361 eaeslkesla aaqmeaeere eaallllkkd tqiaqlegkl qekreelasl vqekvaavde
      421 serfrevnqn lkeivfslsk qleqeqnrre smvtdavqtd gclvpkadce vqtsgnydel
      481 llklessehe vnllkssihr iccdnaalss mykecrgelt kaseichale dqlkrlaeel
      541 kavntdkeal lvktpvkasp rkekhpgsea kqsstsvssv plkmagsrcr vapeqktvdv
      601 sgeavwmtas giaeavgnvr vrsfrgsavp asskkpklek kgd
//
LOCUS       XP_077502963             643 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113647 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502963
VERSION     XP_077502963.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646837.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..643
                     /product="uncharacterized protein LOC144113647 isoform X1"
                     /calculated_mol_wt=71416
     Region          208..>550
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..643
                     /gene="LOC144113647"
                     /coded_by="XM_077646837.1:201..2132"
                     /db_xref="GeneID:144113647"
ORIGIN      
        1 msegqdhhkr mpttqvatvg sanaaegqpg avmtssqqrp cysdlldsir kihdklitlt
       61 lklifklqvc enaeasnqvl ggrlndpqqr ivelmadnaq chlnmqrlve ehsnvladne
      121 slvlqhrtek eekdqliaql rmlasklcdl sdlddrsalk liealgsavq qlrdertsln
      181 dreraaktti ealtgerdal maenrvlaek qkdynelqqa kesledhlti ykdvaknitr
      241 evhhqltsam gtlkqviqes tiaegpfeat lqtvereavr lrtqislfyd hwshservts
      301 dmsdelarkk eevqrlsaec armdsvireq maeieglcsg itnfeqlaad sagdlarvqa
      361 eaeslkesla aaqmeaeere eaallllkkd tqiaqlegkl qekreelasl vqekvaavde
      421 serfrevnqn lkeivfslsk qleqeqnrre smvtdavqtd gclvpkadce vqtsgnydel
      481 llklessehe vnllkssihr iccdnaalss mykecrgelt kaseichale dqlkrlaeel
      541 kavntdkeal lvktpvkasp rkekhpgsea kqsstsvssv plkmagsrcr vapeqktvdv
      601 sgeavwmtas giaeavgnvr vrsfrgsavp asskkpklek kgd
//
LOCUS       XP_077502965             617 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113647 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502965
VERSION     XP_077502965.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646839.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..617
                     /product="uncharacterized protein LOC144113647 isoform X2"
                     /calculated_mol_wt=68684
     Region          208..>550
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..617
                     /gene="LOC144113647"
                     /coded_by="XM_077646839.1:206..2059"
                     /db_xref="GeneID:144113647"
ORIGIN      
        1 msegqdhhkr mpttqvatvg sanaaegqpg avmtssqqrp cysdlldsir kihdklitlt
       61 lklifklqvc enaeasnqvl ggrlndpqqr ivelmadnaq chlnmqrlve ehsnvladne
      121 slvlqhrtek eekdqliaql rmlasklcdl sdlddrsalk liealgsavq qlrdertsln
      181 dreraaktti ealtgerdal maenrvlaek qkdynelqqa kesledhlti ykdvaknitr
      241 evhhqltsam gtlkqviqes tiaegpfeat lqtvereavr lrtqislfyd hwshservts
      301 dmsdelarkk eevqrlsaec armdsvireq maeieglcsg itnfeqlaad sagdlarvqa
      361 eaeslkesla aaqmeaeere eaallllkkd tqiaqlegkl qekreelasl vqekvaavde
      421 serfrevnqn lkeivfslsk qleqeqnrre smvtdavqtd gclvpkadce vqtsgnydel
      481 llklessehe vnllkssihr iccdnaalss mykecrgelt kaseichale dqlkrlaeel
      541 kavntdkeal lvktpvkasp rkekhpgsea kqsstsvssv plkmagsrcr vapeqktvdg
      601 savpasskkp klekkgd
//
LOCUS       XP_077502966             586 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113647 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502966
VERSION     XP_077502966.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646840.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..586
                     /product="uncharacterized protein LOC144113647 isoform X3"
                     /calculated_mol_wt=65424
     Region          <71..>550
                     /region_name="CCDC158"
                     /note="Coiled-coil domain-containing protein 158;
                     pfam15921"
                     /db_xref="CDD:464943"
     CDS             1..586
                     /gene="LOC144113647"
                     /coded_by="XM_077646840.1:205..1965"
                     /db_xref="GeneID:144113647"
ORIGIN      
        1 msegqdhhkr mpttqvatvg sanaaegqpg avmtssqqrp cysdlldsir kihdklitlt
       61 lklifklqvc enaeasnqvl ggrlndpqqr ivelmadnaq chlnmqrlve ehsnvladne
      121 slvlqhrtek eekdqliaql rmlasklcdl sdlddrsalk liealgsavq qlrdertsln
      181 dreraaktti ealtgerdal maenrvlaek qkdynelqqa kesledhlti ykdvaknitr
      241 evhhqltsam gtlkqviqes tiaegpfeat lqtvereavr lrtqislfyd hwshservts
      301 dmsdelarkk eevqrlsaec armdsvireq maeieglcsg itnfeqlaad sagdlarvqa
      361 eaeslkesla aaqmeaeere eaallllkkd tqiaqlegkl qekreelasl vqekvaavde
      421 serfrevnqn lkeivfslsk qleqeqnrre smvtdavqtd gclvpkadce vqtsgnydel
      481 llklessehe vnllkssihr iccdnaalss mykecrgelt kaseichale dqlkrlaeel
      541 kavntdkeal lvktpvkasp rkekhpgsgs avpasskkpk lekkgd
//
LOCUS       XP_077502967             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113648 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502967
VERSION     XP_077502967.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646841.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144113648 isoform X1"
                     /calculated_mol_wt=14961
     Region          104..>141
                     /region_name="BRCT"
                     /note="C-terminal domain of the breast cancer suppressor
                     protein (BRCA1) and related domains; cl00038"
                     /db_xref="CDD:469589"
     CDS             1..141
                     /gene="LOC144113648"
                     /coded_by="XM_077646841.1:1080..1505"
                     /db_xref="GeneID:144113648"
ORIGIN      
        1 mpsifesnna altsaeltve dimssvrkne ecgeenelgd dadsppadqg dkpvssaegp
       61 stklgkrkgn lpddvpvelr rsrsqegeda galigtsetr krkpevlftd idctveeeqv
      121 vrelggtvaa masdcthlit d
//
LOCUS       XP_077502968             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113648 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502968
VERSION     XP_077502968.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646842.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144113648 isoform X1"
                     /calculated_mol_wt=14961
     Region          104..>141
                     /region_name="BRCT"
                     /note="C-terminal domain of the breast cancer suppressor
                     protein (BRCA1) and related domains; cl00038"
                     /db_xref="CDD:469589"
     CDS             1..141
                     /gene="LOC144113648"
                     /coded_by="XM_077646842.1:1080..1505"
                     /db_xref="GeneID:144113648"
ORIGIN      
        1 mpsifesnna altsaeltve dimssvrkne ecgeenelgd dadsppadqg dkpvssaegp
       61 stklgkrkgn lpddvpvelr rsrsqegeda galigtsetr krkpevlftd idctveeeqv
      121 vrelggtvaa masdcthlit d
//
LOCUS       XP_077502969             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113648 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502969
VERSION     XP_077502969.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646843.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144113648 isoform X1"
                     /calculated_mol_wt=14961
     Region          104..>141
                     /region_name="BRCT"
                     /note="C-terminal domain of the breast cancer suppressor
                     protein (BRCA1) and related domains; cl00038"
                     /db_xref="CDD:469589"
     CDS             1..141
                     /gene="LOC144113648"
                     /coded_by="XM_077646843.1:889..1314"
                     /db_xref="GeneID:144113648"
ORIGIN      
        1 mpsifesnna altsaeltve dimssvrkne ecgeenelgd dadsppadqg dkpvssaegp
       61 stklgkrkgn lpddvpvelr rsrsqegeda galigtsetr krkpevlftd idctveeeqv
      121 vrelggtvaa masdcthlit d
//
LOCUS       XP_077502970             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113648 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502970
VERSION     XP_077502970.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646844.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144113648 isoform X1"
                     /calculated_mol_wt=14961
     Region          104..>141
                     /region_name="BRCT"
                     /note="C-terminal domain of the breast cancer suppressor
                     protein (BRCA1) and related domains; cl00038"
                     /db_xref="CDD:469589"
     CDS             1..141
                     /gene="LOC144113648"
                     /coded_by="XM_077646844.1:1080..1505"
                     /db_xref="GeneID:144113648"
ORIGIN      
        1 mpsifesnna altsaeltve dimssvrkne ecgeenelgd dadsppadqg dkpvssaegp
       61 stklgkrkgn lpddvpvelr rsrsqegeda galigtsetr krkpevlftd idctveeeqv
      121 vrelggtvaa masdcthlit d
//
LOCUS       XP_077502971             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113648 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502971
VERSION     XP_077502971.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646845.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144113648 isoform X1"
                     /calculated_mol_wt=14961
     Region          104..>141
                     /region_name="BRCT"
                     /note="C-terminal domain of the breast cancer suppressor
                     protein (BRCA1) and related domains; cl00038"
                     /db_xref="CDD:469589"
     CDS             1..141
                     /gene="LOC144113648"
                     /coded_by="XM_077646845.1:901..1326"
                     /db_xref="GeneID:144113648"
ORIGIN      
        1 mpsifesnna altsaeltve dimssvrkne ecgeenelgd dadsppadqg dkpvssaegp
       61 stklgkrkgn lpddvpvelr rsrsqegeda galigtsetr krkpevlftd idctveeeqv
      121 vrelggtvaa masdcthlit d
//
LOCUS       XP_077502972             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113648 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502972
VERSION     XP_077502972.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646846.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144113648 isoform X1"
                     /calculated_mol_wt=14961
     Region          104..>141
                     /region_name="BRCT"
                     /note="C-terminal domain of the breast cancer suppressor
                     protein (BRCA1) and related domains; cl00038"
                     /db_xref="CDD:469589"
     CDS             1..141
                     /gene="LOC144113648"
                     /coded_by="XM_077646846.1:1080..1505"
                     /db_xref="GeneID:144113648"
ORIGIN      
        1 mpsifesnna altsaeltve dimssvrkne ecgeenelgd dadsppadqg dkpvssaegp
       61 stklgkrkgn lpddvpvelr rsrsqegeda galigtsetr krkpevlftd idctveeeqv
      121 vrelggtvaa masdcthlit d
//
LOCUS       XP_077502973             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113648 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502973
VERSION     XP_077502973.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646847.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144113648 isoform X1"
                     /calculated_mol_wt=14961
     Region          104..>141
                     /region_name="BRCT"
                     /note="C-terminal domain of the breast cancer suppressor
                     protein (BRCA1) and related domains; cl00038"
                     /db_xref="CDD:469589"
     CDS             1..141
                     /gene="LOC144113648"
                     /coded_by="XM_077646847.1:1080..1505"
                     /db_xref="GeneID:144113648"
ORIGIN      
        1 mpsifesnna altsaeltve dimssvrkne ecgeenelgd dadsppadqg dkpvssaegp
       61 stklgkrkgn lpddvpvelr rsrsqegeda galigtsetr krkpevlftd idctveeeqv
      121 vrelggtvaa masdcthlit d
//
LOCUS       XP_077502974             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113648 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502974
VERSION     XP_077502974.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646848.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144113648 isoform X1"
                     /calculated_mol_wt=14961
     Region          104..>141
                     /region_name="BRCT"
                     /note="C-terminal domain of the breast cancer suppressor
                     protein (BRCA1) and related domains; cl00038"
                     /db_xref="CDD:469589"
     CDS             1..141
                     /gene="LOC144113648"
                     /coded_by="XM_077646848.1:1080..1505"
                     /db_xref="GeneID:144113648"
ORIGIN      
        1 mpsifesnna altsaeltve dimssvrkne ecgeenelgd dadsppadqg dkpvssaegp
       61 stklgkrkgn lpddvpvelr rsrsqegeda galigtsetr krkpevlftd idctveeeqv
      121 vrelggtvaa masdcthlit d
//
LOCUS       XP_077502975             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113648 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502975
VERSION     XP_077502975.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646849.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144113648 isoform X1"
                     /calculated_mol_wt=14961
     Region          104..>141
                     /region_name="BRCT"
                     /note="C-terminal domain of the breast cancer suppressor
                     protein (BRCA1) and related domains; cl00038"
                     /db_xref="CDD:469589"
     CDS             1..141
                     /gene="LOC144113648"
                     /coded_by="XM_077646849.1:630..1055"
                     /db_xref="GeneID:144113648"
ORIGIN      
        1 mpsifesnna altsaeltve dimssvrkne ecgeenelgd dadsppadqg dkpvssaegp
       61 stklgkrkgn lpddvpvelr rsrsqegeda galigtsetr krkpevlftd idctveeeqv
      121 vrelggtvaa masdcthlit d
//
LOCUS       XP_077502976             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113648 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502976
VERSION     XP_077502976.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646850.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144113648 isoform X1"
                     /calculated_mol_wt=14961
     Region          104..>141
                     /region_name="BRCT"
                     /note="C-terminal domain of the breast cancer suppressor
                     protein (BRCA1) and related domains; cl00038"
                     /db_xref="CDD:469589"
     CDS             1..141
                     /gene="LOC144113648"
                     /coded_by="XM_077646850.1:1080..1505"
                     /db_xref="GeneID:144113648"
ORIGIN      
        1 mpsifesnna altsaeltve dimssvrkne ecgeenelgd dadsppadqg dkpvssaegp
       61 stklgkrkgn lpddvpvelr rsrsqegeda galigtsetr krkpevlftd idctveeeqv
      121 vrelggtvaa masdcthlit d
//
LOCUS       XP_077502977             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113648 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502977
VERSION     XP_077502977.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646851.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144113648 isoform X1"
                     /calculated_mol_wt=14961
     Region          104..>141
                     /region_name="BRCT"
                     /note="C-terminal domain of the breast cancer suppressor
                     protein (BRCA1) and related domains; cl00038"
                     /db_xref="CDD:469589"
     CDS             1..141
                     /gene="LOC144113648"
                     /coded_by="XM_077646851.1:1080..1505"
                     /db_xref="GeneID:144113648"
ORIGIN      
        1 mpsifesnna altsaeltve dimssvrkne ecgeenelgd dadsppadqg dkpvssaegp
       61 stklgkrkgn lpddvpvelr rsrsqegeda galigtsetr krkpevlftd idctveeeqv
      121 vrelggtvaa masdcthlit d
//
LOCUS       XP_077502978             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113648 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502978
VERSION     XP_077502978.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646852.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144113648 isoform X1"
                     /calculated_mol_wt=14961
     Region          104..>141
                     /region_name="BRCT"
                     /note="C-terminal domain of the breast cancer suppressor
                     protein (BRCA1) and related domains; cl00038"
                     /db_xref="CDD:469589"
     CDS             1..141
                     /gene="LOC144113648"
                     /coded_by="XM_077646852.1:1080..1505"
                     /db_xref="GeneID:144113648"
ORIGIN      
        1 mpsifesnna altsaeltve dimssvrkne ecgeenelgd dadsppadqg dkpvssaegp
       61 stklgkrkgn lpddvpvelr rsrsqegeda galigtsetr krkpevlftd idctveeeqv
      121 vrelggtvaa masdcthlit d
//
LOCUS       XP_077502980             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113648 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502980
VERSION     XP_077502980.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646854.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144113648 isoform X1"
                     /calculated_mol_wt=14961
     Region          104..>141
                     /region_name="BRCT"
                     /note="C-terminal domain of the breast cancer suppressor
                     protein (BRCA1) and related domains; cl00038"
                     /db_xref="CDD:469589"
     CDS             1..141
                     /gene="LOC144113648"
                     /coded_by="XM_077646854.1:1080..1505"
                     /db_xref="GeneID:144113648"
ORIGIN      
        1 mpsifesnna altsaeltve dimssvrkne ecgeenelgd dadsppadqg dkpvssaegp
       61 stklgkrkgn lpddvpvelr rsrsqegeda galigtsetr krkpevlftd idctveeeqv
      121 vrelggtvaa masdcthlit d
//
LOCUS       XP_077502981             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113648 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502981
VERSION     XP_077502981.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646855.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144113648 isoform X1"
                     /calculated_mol_wt=14961
     Region          104..>141
                     /region_name="BRCT"
                     /note="C-terminal domain of the breast cancer suppressor
                     protein (BRCA1) and related domains; cl00038"
                     /db_xref="CDD:469589"
     CDS             1..141
                     /gene="LOC144113648"
                     /coded_by="XM_077646855.1:1080..1505"
                     /db_xref="GeneID:144113648"
ORIGIN      
        1 mpsifesnna altsaeltve dimssvrkne ecgeenelgd dadsppadqg dkpvssaegp
       61 stklgkrkgn lpddvpvelr rsrsqegeda galigtsetr krkpevlftd idctveeeqv
      121 vrelggtvaa masdcthlit d
//
LOCUS       XP_077502982             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113648 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502982
VERSION     XP_077502982.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646856.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144113648 isoform X1"
                     /calculated_mol_wt=14961
     Region          104..>141
                     /region_name="BRCT"
                     /note="C-terminal domain of the breast cancer suppressor
                     protein (BRCA1) and related domains; cl00038"
                     /db_xref="CDD:469589"
     CDS             1..141
                     /gene="LOC144113648"
                     /coded_by="XM_077646856.1:1080..1505"
                     /db_xref="GeneID:144113648"
ORIGIN      
        1 mpsifesnna altsaeltve dimssvrkne ecgeenelgd dadsppadqg dkpvssaegp
       61 stklgkrkgn lpddvpvelr rsrsqegeda galigtsetr krkpevlftd idctveeeqv
      121 vrelggtvaa masdcthlit d
//
LOCUS       XP_077502983             140 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113648 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502983
VERSION     XP_077502983.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646857.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..140
                     /product="uncharacterized protein LOC144113648 isoform X2"
                     /calculated_mol_wt=14833
     Region          103..>140
                     /region_name="BRCT"
                     /note="C-terminal domain of the breast cancer suppressor
                     protein (BRCA1) and related domains; cl00038"
                     /db_xref="CDD:469589"
     CDS             1..140
                     /gene="LOC144113648"
                     /coded_by="XM_077646857.1:1080..1502"
                     /db_xref="GeneID:144113648"
ORIGIN      
        1 mpsifesnna altsaeltve dimssvrkne ecgeenelgd dadsppadqg dkpvssaegp
       61 stklgkrkgn lpddvpvelr rsrsqegeda galigtsetr krkpevlftd idctveeevv
      121 relggtvaam asdcthlitd
//
LOCUS       XP_077502984             167 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113652 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502984
VERSION     XP_077502984.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646858.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..167
                     /product="uncharacterized protein LOC144113652 isoform X1"
                     /calculated_mol_wt=18969
     Region          87..162
                     /region_name="Death"
                     /note="Death Domain: a protein-protein interaction domain;
                     cd01670"
                     /db_xref="CDD:260017"
     CDS             1..167
                     /gene="LOC144113652"
                     /coded_by="XM_077646858.1:371..874"
                     /db_xref="GeneID:144113652"
ORIGIN      
        1 mkcvqqstrv aakyrsvrdh laslqynhth sqhhdsvpgi hrdakdpaqd isevhgharl
       61 nmkddsyiqd sipgdilsst rkisktdiey vsdevsewre larllpedpi teeelhsikr
      121 lhhssrdrvr svlelwrhkc hgsatlgslv eslhllgyse lamtlkp
//
LOCUS       XP_077502985             161 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113652 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502985
VERSION     XP_077502985.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646859.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..161
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..161
                     /product="uncharacterized protein LOC144113652 isoform X2"
                     /calculated_mol_wt=18004
     Region          90..160
                     /region_name="Death"
                     /note="Death domain; pfam00531"
                     /db_xref="CDD:459845"
     CDS             1..161
                     /gene="LOC144113652"
                     /coded_by="XM_077646859.1:309..794"
                     /db_xref="GeneID:144113652"
ORIGIN      
        1 madteivqph tqsasrlssw npqrwrggga slsalayakd paqdisevhg harlnmkdds
       61 yiqdsipgdi lsstrkiskt dieyvsdevs ewrelarllp edpiteeelh sikrlhhssr
      121 drvrsvlelw rhkchgsatl gslveslhll gyselamtlk p
//
LOCUS       XP_077502986             138 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113652 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077502986
VERSION     XP_077502986.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646860.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..138
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..138
                     /product="uncharacterized protein LOC144113652 isoform X3"
                     /calculated_mol_wt=15688
     CDS             1..138
                     /gene="LOC144113652"
                     /coded_by="XM_077646860.1:371..787"
                     /db_xref="GeneID:144113652"
ORIGIN      
        1 mkcvqqstrv aakyrsvrdh laslqynhth sqhhdsvpgi hrdakdpaqd isevhgharl
       61 nmkddsyiqd sipgdilsst rkisktdiey vsdevsewre larllpgfce kknlnkkets
      121 wsvkvkqdlf athrgsdh
//
LOCUS       XP_077502987             131 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113652 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077502987
VERSION     XP_077502987.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646861.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..131
                     /product="uncharacterized protein LOC144113652 isoform X4"
                     /calculated_mol_wt=14768
     Region          60..130
                     /region_name="Death"
                     /note="Death domain; pfam00531"
                     /db_xref="CDD:459845"
     CDS             1..131
                     /gene="LOC144113652"
                     /coded_by="XM_077646861.1:309..704"
                     /db_xref="GeneID:144113652"
ORIGIN      
        1 madteidakd paqdisevhg harlnmkdds yiqdsipgdi lsstrkiskt dieyvsdevs
       61 ewrelarllp edpiteeelh sikrlhhssr drvrsvlelw rhkchgsatl gslveslhll
      121 gyselamtlk p
//
LOCUS       XP_077502988             148 aa            linear   INV 25-NOV-2025
DEFINITION  CCAAT/enhancer-binding protein gamma-like [Amblyomma americanum].
ACCESSION   XP_077502988
VERSION     XP_077502988.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646862.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="CCAAT/enhancer-binding protein gamma-like"
                     /calculated_mol_wt=16153
     Region          42..102
                     /region_name="bZIP_CEBPG"
                     /note="Basic leucine zipper (bZIP) domain of
                     CCAAT/enhancer-binding protein gamma (CEBPG): a
                     DNA-binding and dimerization domain; cd14713"
                     /db_xref="CDD:269861"
     Region          44..102
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269861"
     Site            order(47..48,50..51,53..55,57..62,64,68)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269861"
     Site            order(69,72,75..76,79..80,82..83,85..87,89..90,93..94,
                     96..97,100..101)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269861"
     CDS             1..148
                     /gene="LOC144113653"
                     /coded_by="XM_077646862.1:307..753"
                     /db_xref="GeneID:144113653"
ORIGIN      
        1 mpckrdpeww ealmaprkqa sgnssdssss mgrgkrsalm dkdseeyrmh rarnnlavkk
       61 srtkskqraq etqqkvaqlr aenealvaki kilnkelsfl kdlflahaga tggnrgnnct
      121 glegvdlsfl nedeaseskg iasknvgs
//
LOCUS       XP_077502989             148 aa            linear   INV 25-NOV-2025
DEFINITION  CCAAT/enhancer-binding protein gamma-like [Amblyomma americanum].
ACCESSION   XP_077502989
VERSION     XP_077502989.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646863.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="CCAAT/enhancer-binding protein gamma-like"
                     /calculated_mol_wt=16153
     Region          42..102
                     /region_name="bZIP_CEBPG"
                     /note="Basic leucine zipper (bZIP) domain of
                     CCAAT/enhancer-binding protein gamma (CEBPG): a
                     DNA-binding and dimerization domain; cd14713"
                     /db_xref="CDD:269861"
     Region          44..102
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269861"
     Site            order(47..48,50..51,53..55,57..62,64,68)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269861"
     Site            order(69,72,75..76,79..80,82..83,85..87,89..90,93..94,
                     96..97,100..101)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269861"
     CDS             1..148
                     /gene="LOC144113653"
                     /coded_by="XM_077646863.1:311..757"
                     /db_xref="GeneID:144113653"
ORIGIN      
        1 mpckrdpeww ealmaprkqa sgnssdssss mgrgkrsalm dkdseeyrmh rarnnlavkk
       61 srtkskqraq etqqkvaqlr aenealvaki kilnkelsfl kdlflahaga tggnrgnnct
      121 glegvdlsfl nedeaseskg iasknvgs
//
LOCUS       XP_077502990             148 aa            linear   INV 25-NOV-2025
DEFINITION  CCAAT/enhancer-binding protein gamma-like [Amblyomma americanum].
ACCESSION   XP_077502990
VERSION     XP_077502990.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646864.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="CCAAT/enhancer-binding protein gamma-like"
                     /calculated_mol_wt=16153
     Region          42..102
                     /region_name="bZIP_CEBPG"
                     /note="Basic leucine zipper (bZIP) domain of
                     CCAAT/enhancer-binding protein gamma (CEBPG): a
                     DNA-binding and dimerization domain; cd14713"
                     /db_xref="CDD:269861"
     Region          44..102
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269861"
     Site            order(47..48,50..51,53..55,57..62,64,68)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269861"
     Site            order(69,72,75..76,79..80,82..83,85..87,89..90,93..94,
                     96..97,100..101)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269861"
     CDS             1..148
                     /gene="LOC144113653"
                     /coded_by="XM_077646864.1:169..615"
                     /db_xref="GeneID:144113653"
ORIGIN      
        1 mpckrdpeww ealmaprkqa sgnssdssss mgrgkrsalm dkdseeyrmh rarnnlavkk
       61 srtkskqraq etqqkvaqlr aenealvaki kilnkelsfl kdlflahaga tggnrgnnct
      121 glegvdlsfl nedeaseskg iasknvgs
//
LOCUS       XP_077502991             148 aa            linear   INV 25-NOV-2025
DEFINITION  CCAAT/enhancer-binding protein gamma-like [Amblyomma americanum].
ACCESSION   XP_077502991
VERSION     XP_077502991.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646865.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="CCAAT/enhancer-binding protein gamma-like"
                     /calculated_mol_wt=16153
     Region          42..102
                     /region_name="bZIP_CEBPG"
                     /note="Basic leucine zipper (bZIP) domain of
                     CCAAT/enhancer-binding protein gamma (CEBPG): a
                     DNA-binding and dimerization domain; cd14713"
                     /db_xref="CDD:269861"
     Region          44..102
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269861"
     Site            order(47..48,50..51,53..55,57..62,64,68)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269861"
     Site            order(69,72,75..76,79..80,82..83,85..87,89..90,93..94,
                     96..97,100..101)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269861"
     CDS             1..148
                     /gene="LOC144113653"
                     /coded_by="XM_077646865.1:162..608"
                     /db_xref="GeneID:144113653"
ORIGIN      
        1 mpckrdpeww ealmaprkqa sgnssdssss mgrgkrsalm dkdseeyrmh rarnnlavkk
       61 srtkskqraq etqqkvaqlr aenealvaki kilnkelsfl kdlflahaga tggnrgnnct
      121 glegvdlsfl nedeaseskg iasknvgs
//
LOCUS       XP_077502992             502 aa            linear   INV 25-NOV-2025
DEFINITION  RNA-binding protein 39-like protein Caper isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077502992
VERSION     XP_077502992.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646866.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..502
                     /product="RNA-binding protein 39-like protein Caper
                     isoform X1"
                     /calculated_mol_wt=55712
     Region          <132..495
                     /region_name="SF-CC1"
                     /note="splicing factor, CC1-like family; TIGR01622"
                     /db_xref="CDD:273721"
     CDS             1..502
                     /gene="Caper"
                     /coded_by="XM_077646866.1:148..1656"
                     /db_xref="GeneID:144113655"
ORIGIN      
        1 maddldveal leapyrkeev ngagslqqag ssqegakgsa dkenrhgsss sskkrrsrsr
       61 srsrgrgrhr srsrgrdkrr srsrsrerrr rhsrspgrrr gpplpprppr srsrsrdrrr
      121 rrsmsprrrg ldditpeerd mrtvfcmqls qrirardlee ffsavgkvrd vrlimdnktr
      181 rskgiayvef ldvesvplam glngqklfgi pivvqptqae rnraaaqnas tssstlqrgn
      241 vgpmrlyvgs lhfnitedml kgifepfgki dkielikdme tnrskgygfi tfhdsedakk
      301 aleqlngfel agrpmkvghv tertdvsqap sfldseeldr sgidlgatgr lqlmaklaeg
      361 tgfqipqaav nalqmnptgl pgqpqaaava aaaaaptiat qcfllsnmfd pltetnpswd
      421 eeirrdviee crkhggalhv yvdraspegh vyvkcptias avasvnalhg rwfagriita
      481 ayvpvmsyht lfpdsattna ll
//
LOCUS       XP_077502994             488 aa            linear   INV 25-NOV-2025
DEFINITION  RNA-binding protein 39-like protein Caper isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077502994
VERSION     XP_077502994.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646868.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..488
                     /product="RNA-binding protein 39-like protein Caper
                     isoform X2"
                     /calculated_mol_wt=54204
     Region          <118..481
                     /region_name="SF-CC1"
                     /note="splicing factor, CC1-like family; TIGR01622"
                     /db_xref="CDD:273721"
     CDS             1..488
                     /gene="Caper"
                     /coded_by="XM_077646868.1:14..1480"
                     /db_xref="GeneID:144113655"
ORIGIN      
        1 myqeevngag slqqagssqe gakgsadken rhgssssskk rrsrsrsrsr grgrhrsrsr
       61 grdkrrsrsr srerrrrhsr spgrrrgppl pprpprsrsr srdrrrrrsm sprrrglddi
      121 tpeerdmrtv fcmqlsqrir ardleeffsa vgkvrdvrli mdnktrrskg iayvefldve
      181 svplamglng qklfgipivv qptqaernra aaqnastsss tlqrgnvgpm rlyvgslhfn
      241 itedmlkgif epfgkidkie likdmetnrs kgygfitfhd sedakkaleq lngfelagrp
      301 mkvghvtert dvsqapsfld seeldrsgid lgatgrlqlm aklaegtgfq ipqaavnalq
      361 mnptglpgqp qaaavaaaaa aptiatqcfl lsnmfdplte tnpswdeeir rdvieecrkh
      421 ggalhvyvdr aspeghvyvk cptiasavas vnalhgrwfa griitaayvp vmsyhtlfpd
      481 sattnall
//
LOCUS       XP_077502995             392 aa            linear   INV 25-NOV-2025
DEFINITION  G protein alpha s subunit isoform X1 [Amblyomma americanum].
ACCESSION   XP_077502995
VERSION     XP_077502995.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646869.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..392
                     /product="G protein alpha s subunit isoform X1"
                     /calculated_mol_wt=45762
     Region          41..386
                     /region_name="G-alpha"
                     /note="Alpha subunit of G proteins (guanine nucleotide
                     binding); cd00066"
                     /db_xref="CDD:206639"
     Site            order(47,52..54,189,208,211,277..278,280,363..364)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206639"
     Site            47..54
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206639"
     Site            order(48..50,78,82,86,89,92..93,113,116,157,186,211,219,
                     223)
                     /site_type="active"
                     /note="GoLoco binding site [active]"
                     /db_xref="CDD:206639"
     Site            184..192
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206639"
     Site            189
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206639"
     Site            order(190,192,194,205,207,211..213,215,218..219,221..224)
                     /site_type="other"
                     /note="beta - gamma complex interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206639"
     Site            order(192,217,220..221,224)
                     /site_type="other"
                     /note="adenylyl cyclase interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206639"
     Site            208..211
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206639"
     Site            209..225
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206639"
     Site            277..280
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206639"
     Site            order(355..366,368..371)
                     /site_type="active"
                     /note="putative receptor binding site [active]"
                     /db_xref="CDD:206639"
     Site            363..365
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206639"
     CDS             1..392
                     /gene="Galphas"
                     /coded_by="XM_077646869.1:254..1432"
                     /db_xref="GeneID:144113658"
ORIGIN      
        1 mgcfggsssk sdaeedkrrk eankkiekqi qkdkqiyrat hrllllgage sgkstivkqm
       61 rilhvngfse eekkqkiedi kknirdailt itgamstlvp pvqleksenq wrvdyiqdva
      121 sspdfdypae fyehteilwk dkgvqaafer sneyqlidca kyfldrvati kqpdytpneq
      181 dilrcrvlts gifetkfqvd kvnfhmfdvg gqrderrkwi qcfndvtaii fvtacssynm
      241 vlredpnqnr lresldlfks iwnnrwlrti svilflnkqd llaekikagk srleeyfqef
      301 ahyqtpsdep rlnpfqpgtp htvietgedp evirakyfir deflristas gdgkhycyph
      361 ftcavdteni rrvfndcrdi iqrmhlrqye ll
//
LOCUS       XP_077502996             379 aa            linear   INV 25-NOV-2025
DEFINITION  G protein alpha s subunit isoform X2 [Amblyomma americanum].
ACCESSION   XP_077502996
VERSION     XP_077502996.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646870.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..379
                     /product="G protein alpha s subunit isoform X2"
                     /calculated_mol_wt=44260
     Region          41..373
                     /region_name="G-alpha"
                     /note="Alpha subunit of G proteins (guanine nucleotide
                     binding); cd00066"
                     /db_xref="CDD:206639"
     Site            order(47,52..54,189,208,211,277..278,280,350..351)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206639"
     Site            47..54
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206639"
     Site            order(48..50,78,82,86,89,92..93,113,116,157,186,211,219,
                     223)
                     /site_type="active"
                     /note="GoLoco binding site [active]"
                     /db_xref="CDD:206639"
     Site            184..192
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206639"
     Site            189
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206639"
     Site            order(190,192,194,205,207,211..213,215,218..219,221..224)
                     /site_type="other"
                     /note="beta - gamma complex interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206639"
     Site            order(192,217,220..221,224)
                     /site_type="other"
                     /note="adenylyl cyclase interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206639"
     Site            208..211
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206639"
     Site            209..225
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206639"
     Site            277..280
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206639"
     Site            order(342..353,355..358)
                     /site_type="active"
                     /note="putative receptor binding site [active]"
                     /db_xref="CDD:206639"
     Site            350..352
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206639"
     CDS             1..379
                     /gene="Galphas"
                     /coded_by="XM_077646870.1:234..1373"
                     /db_xref="GeneID:144113658"
ORIGIN      
        1 mgcfggsssk sdaeedkrrk eankkiekqi qkdkqiyrat hrllllgage sgkstivkqm
       61 rilhvngfse eekkqkiedi kknirdailt itgamstlvp pvqleksenq wrvdyiqdva
      121 sspdfdypae fyehteilwk dkgvqaafer sneyqlidca kyfldrvati kqpdytpneq
      181 dilrcrvlts gifetkfqvd kvnfhmfdvg gqrderrkwi qcfndvtaii fvtacssynm
      241 vlredpnqnr lresldlfks iwnnrwlrti svilflnkqd llaekikagk srleeyfqef
      301 ahyqtpsdav ietgedpevi rakyfirdef lristasgdg khycyphftc avdtenirrv
      361 fndcrdiiqr mhlrqyell
//
LOCUS       XP_077502997             218 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113659 [Amblyomma americanum].
ACCESSION   XP_077502997
VERSION     XP_077502997.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646871.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..218
                     /product="uncharacterized protein LOC144113659"
                     /calculated_mol_wt=23689
     Region          54..208
                     /region_name="GSH_Peroxidase"
                     /note="Glutathione (GSH) peroxidase family; tetrameric
                     selenoenzymes that catalyze the reduction of a variety of
                     hydroperoxides including lipid peroxidases, using GSH as a
                     specific electron donor substrate. GSH peroxidase contains
                     one selenocysteine residue per...; cd00340"
                     /db_xref="CDD:238207"
     Site            order(87,124,178)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:238207"
     Site            order(123,126,128,131,134,138)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:238207"
     CDS             1..218
                     /gene="LOC144113659"
                     /coded_by="XM_077646871.1:78..734"
                     /db_xref="GeneID:144113659"
ORIGIN      
        1 mqvlfrlvvc alciagvlvl vegdpqscpa npadeplmpv ldmasgdnwk dacsiydfta
       61 edisgkkvsl rkysghvvli vnvasrcgft nanyeqlqal hdkyssnnpp lsilgfpcnq
      121 fgsqepgsna eisdfcrsty nvkfdmfakv dvngdgahpl wkflkhrqsg tlgdgikwnf
      181 tkflvnksgq pvaryaptte pnaiendikk llagasql
//
LOCUS       XP_077502998             524 aa            linear   INV 25-NOV-2025
DEFINITION  alanine aminotransferase 2 [Amblyomma americanum].
ACCESSION   XP_077502998
VERSION     XP_077502998.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646872.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..524
                     /product="alanine aminotransferase 2"
                     /calculated_mol_wt=57802
     Region          49..524
                     /region_name="PTZ00377"
                     /note="alanine aminotransferase; Provisional"
                     /db_xref="CDD:240391"
     CDS             1..524
                     /gene="LOC144113662"
                     /coded_by="XM_077646872.1:217..1791"
                     /db_xref="GeneID:144113662"
ORIGIN      
        1 mlqramfrqv rllsearlsr dprlwaaatk vnfplgaavg raastmagrt vtldnmnphl
       61 itmeyavrgp ivirageiek elkagkkynf teviranigd chavgqvplt ffrqviaact
      121 vpdlmkgdmf padvklraqq ildycggksv gaysdsagve iirrhvadyi sqrdgveaky
      181 edvllstgas esvrsvlnll nvrkdgkpsg vmipipqypl ysatlaeygi hqanyyldee
      241 ndwalsiqel eralseakkv cnprglvvin pgnptgsvlt eqnikqvvef ahkhklflma
      301 devyqhniya egaafhsfrk vmhnmgppys emelasfmsa skgymgecgl rggyvevinl
      361 dpavksvllk svsaklcssv lgqcamdcvv nppkpgepsy dlfikektav lnslkkraql
      421 vadtfnsfeg frcnkvagam yafprftlpq kaieeakaag qapdffyvmq llentgvcvv
      481 pgsgfgqvpg tyhfrttilp pleklqvmlt hfkdfhqkfm skyk
//
LOCUS       XP_077502999             318 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113663 [Amblyomma americanum].
ACCESSION   XP_077502999
VERSION     XP_077502999.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646873.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..318
                     /product="uncharacterized protein LOC144113663"
                     /calculated_mol_wt=36376
     CDS             1..318
                     /gene="LOC144113663"
                     /coded_by="XM_077646873.1:1009..1965"
                     /db_xref="GeneID:144113663"
ORIGIN      
        1 mecsmsdamk avaedcsdde flkhpellev leklkeqaey vvtngtrrkl ekakadafaa
       61 qerhlqalll knawtnigis lcskedlepc vegatkdfie qfkglkdlvl iaesrfgsvl
      121 niqsqpevtq elkaeihill aeellkqksk llnqmgdfkc sslqesvvsr cidrdvkeks
      181 vfnlmreyan casefkksye eyltvvssmp vldeeehdll kkceevyeny flavdeieee
      241 vescienevp dlfnetekrl lceieqktaa leekesriqe feriwspele dkitsykiai
      301 rdnrglqfal dtastdsd
//
LOCUS       XP_077503000             318 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113664 [Amblyomma americanum].
ACCESSION   XP_077503000
VERSION     XP_077503000.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646874.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..318
                     /product="uncharacterized protein LOC144113664"
                     /calculated_mol_wt=36423
     CDS             1..318
                     /gene="LOC144113664"
                     /coded_by="XM_077646874.1:150..1106"
                     /db_xref="GeneID:144113664"
ORIGIN      
        1 meatmsdafk avtedcsddd flkhpellev leklkeraey vvtndtrrkl ekakadalaa
       61 qerhlqalvl knawtnigis lcskedlepc vegatkdfie qfeglkyqvl iaespfgsvl
      121 niqnqpevtq elkaevhiql aeellkqksk llnqmgdfkc sslqesvlsk ctdrdikkrs
      181 vfnlvleyad casefkkaye qyltlvssmp vldekehdll kiceevyeny flandgieee
      241 velciqhnvn hlfkeaekrl lceveqkrat vqqkesrlqe feriwspele dkitsykiai
      301 rdnrglkfal dtassdsd
//
LOCUS       XP_077503002             203 aa            linear   INV 25-NOV-2025
DEFINITION  NFKB inhibitor interacting Ras like 1 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503002
VERSION     XP_077503002.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646876.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..203
                     /product="NFKB inhibitor interacting Ras like 1 isoform
                     X1"
                     /calculated_mol_wt=22489
     Region          15..178
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            20..27
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206648"
     Site            order(22..28,73,130..131,133,160..162)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206648"
     Site            47
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206648"
     Site            50..52
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206648"
     Site            70..73
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206648"
     Site            order(72..73,90..91)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206648"
     Site            130..133
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206648"
     Site            160..162
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206648"
     CDS             1..203
                     /gene="kappaB-Ras"
                     /coded_by="XM_077646876.1:1..612"
                     /db_xref="GeneID:144113665"
ORIGIN      
        1 mqmlkssvss gktskimvcg hrgcgktsvl eqliynhhpl kadiqptied iycalidndr
       61 ggkekvlfyd srgvessrae vskshlshad gvvliyains resfllleql kkdidkhkdk
      121 kelaflvlgn kadlsaerqv dvqsainwat kekvrfaevs afdrksliep fiylvskinp
      181 ppskstfpql grkgkagsit mel
//
LOCUS       XP_077503003             201 aa            linear   INV 25-NOV-2025
DEFINITION  NFKB inhibitor interacting Ras like 1 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503003
VERSION     XP_077503003.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646877.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..201
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..201
                     /product="NFKB inhibitor interacting Ras like 1 isoform
                     X2"
                     /calculated_mol_wt=22230
     Region          13..176
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            18..25
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206648"
     Site            order(20..26,71,128..129,131,158..160)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206648"
     Site            45
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206648"
     Site            48..50
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206648"
     Site            68..71
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206648"
     Site            order(70..71,88..89)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206648"
     Site            128..131
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206648"
     Site            158..160
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206648"
     CDS             1..201
                     /gene="kappaB-Ras"
                     /coded_by="XM_077646877.1:214..819"
                     /db_xref="GeneID:144113665"
ORIGIN      
        1 mlkssvssgk tskimvcghr gcgktsvleq liynhhplka diqptiediy calidndrgg
       61 kekvlfydsr gvessraevs kshlshadgv vliyainsre sfllleqlkk didkhkdkke
      121 laflvlgnka dlsaerqvdv qsainwatke kvrfaevsaf drksliepfi ylvskinppp
      181 skstfpqlgr kgkagsitme l
//
LOCUS       XP_077503004             201 aa            linear   INV 25-NOV-2025
DEFINITION  NFKB inhibitor interacting Ras like 1 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503004
VERSION     XP_077503004.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646878.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..201
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..201
                     /product="NFKB inhibitor interacting Ras like 1 isoform
                     X2"
                     /calculated_mol_wt=22230
     Region          13..176
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            18..25
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206648"
     Site            order(20..26,71,128..129,131,158..160)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206648"
     Site            45
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206648"
     Site            48..50
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206648"
     Site            68..71
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206648"
     Site            order(70..71,88..89)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206648"
     Site            128..131
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206648"
     Site            158..160
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206648"
     CDS             1..201
                     /gene="kappaB-Ras"
                     /coded_by="XM_077646878.1:245..850"
                     /db_xref="GeneID:144113665"
ORIGIN      
        1 mlkssvssgk tskimvcghr gcgktsvleq liynhhplka diqptiediy calidndrgg
       61 kekvlfydsr gvessraevs kshlshadgv vliyainsre sfllleqlkk didkhkdkke
      121 laflvlgnka dlsaerqvdv qsainwatke kvrfaevsaf drksliepfi ylvskinppp
      181 skstfpqlgr kgkagsitme l
//
LOCUS       XP_077503005             201 aa            linear   INV 25-NOV-2025
DEFINITION  NFKB inhibitor interacting Ras like 1 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503005
VERSION     XP_077503005.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646879.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..201
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..201
                     /product="NFKB inhibitor interacting Ras like 1 isoform
                     X2"
                     /calculated_mol_wt=22230
     Region          13..176
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            18..25
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206648"
     Site            order(20..26,71,128..129,131,158..160)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206648"
     Site            45
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206648"
     Site            48..50
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206648"
     Site            68..71
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206648"
     Site            order(70..71,88..89)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206648"
     Site            128..131
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206648"
     Site            158..160
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206648"
     CDS             1..201
                     /gene="kappaB-Ras"
                     /coded_by="XM_077646879.1:109..714"
                     /db_xref="GeneID:144113665"
ORIGIN      
        1 mlkssvssgk tskimvcghr gcgktsvleq liynhhplka diqptiediy calidndrgg
       61 kekvlfydsr gvessraevs kshlshadgv vliyainsre sfllleqlkk didkhkdkke
      121 laflvlgnka dlsaerqvdv qsainwatke kvrfaevsaf drksliepfi ylvskinppp
      181 skstfpqlgr kgkagsitme l
//
LOCUS       XP_077503006             172 aa            linear   INV 25-NOV-2025
DEFINITION  NFKB inhibitor interacting Ras like 1 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503006
VERSION     XP_077503006.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646880.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..172
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..172
                     /product="NFKB inhibitor interacting Ras like 1 isoform
                     X3"
                     /calculated_mol_wt=18884
     Region          13..149
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            18..25
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206648"
     Site            45
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206648"
     Site            48..50
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206648"
     Site            68..71
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206648"
     Site            order(70..71,88..89)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206648"
     Site            128..131
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206648"
     CDS             1..172
                     /gene="kappaB-Ras"
                     /coded_by="XM_077646880.1:217..735"
                     /db_xref="GeneID:144113665"
ORIGIN      
        1 mlkssvssgk tskimvcghr gcgktsvleq liynhhplka diqptiediy calidndrgg
       61 kekvlfydsr gvessraevs kshlshadgv vliyainsre sfllleqlkk didkhkdkke
      121 laflvlgnka dlsaerqvdv qsainwatke kgkstfpqlg rkgkagsitm el
//
LOCUS       XP_077503007             654 aa            linear   INV 25-NOV-2025
DEFINITION  HEAT repeat-containing protein 3 [Amblyomma americanum].
ACCESSION   XP_077503007
VERSION     XP_077503007.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646881.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..654
                     /product="HEAT repeat-containing protein 3"
                     /calculated_mol_wt=70274
     Region          2..642
                     /region_name="Syo1_like"
                     /note="Fungal symportin 1 (syo1) and similar proteins;
                     cd13394"
                     /db_xref="CDD:240521"
     Site            order(333,340,349..350,384,433,436..437,440..441,443..445,
                     453,484,488,526,531,618,624)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:240521"
     CDS             1..654
                     /gene="LOC144113666"
                     /coded_by="XM_077646881.1:105..2069"
                     /db_xref="GeneID:144113666"
ORIGIN      
        1 mgkaktkkhi srrnnptgmp sthdieedge lqdgnatsss vddlpdmlqg gnaeerecaa
       61 cimanfakdp atceslltas ivrtaaplll dknhavrhsi agalrnisai nheacnalid
      121 fdvmtplvgl lkeynspwqp aesqekidsk tevffeavnl lwnlcesnet svsifnherl
      181 wtvllpclnv ekygtkiata vghclhtvse dnaelaesms gfkassgifq tlsqepgial
      241 fqvlsagili niteaqkvpl apallsiisv lanalslpvl dnikmlaakv aeleepaekk
      301 nmdthvveek ekdhekilas ildglmakqt aleilsnlcc gedggngddd sedvsddssd
      361 vsdgdmetee tsaifplnis cefhealvgq nivpkvldhv sqmdeaiasi llehkpssvv
      421 lrrvhavrcr allcignlaa aldpddlggi sglvstwtcl aqlafvhmdy kdlelleast
      481 sacrsvlqal asaaahggts dtpplpsisd eelkvlaqvg lqcsepavrt nvtrimatlg
      541 cllgphqtnv lkkvgqclle islkdpdivv vtealdalfd vfgedttdti aaeielvtkl
      601 rqmlpafksk vnqakktlay hypviattkt nlvrflkyka qrrgiqngcg gdig
//
LOCUS       XP_077503008             920 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger protein Lobe isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503008
VERSION     XP_077503008.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646882.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..920
                     /product="zinc finger protein Lobe isoform X1"
                     /calculated_mol_wt=99576
     Region          170..190
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(170,173,186,190)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(175,177,179,181..182,185..186,189,203,205,209..210,
                     213..214,217,231,233,235,237..238,241..242,245)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          198..218
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          221..656
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          226..246
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          254..274
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          486..506
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(486,489,502,506)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(491,493,495,497..498,501..502,505,520,522,526..527,
                     530..531,534,550,552,554,556..557,560..561)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          514..535
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          545..562
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          603..624
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(603,606,619,624)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(608,610,612,614..615,618..619,623,638,640,644..645,
                     648..649,652,664,666,668,670..671,674..675)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          633..653
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          659..676
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          705..726
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(705,708,721,726)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(710,712,714,716..717,720..721,725,743,745,749..750,
                     753..754,757,772,774,776,778..779,782..783,786)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          738..758
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          767..787
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..920
                     /gene="L"
                     /coded_by="XM_077646882.1:948..3710"
                     /db_xref="GeneID:144113667"
ORIGIN      
        1 msrrkqakpr alkpdedpge leaseqrrcq amqvdsrpee aersqedssp edccpldgla
       61 qgpeasgdav raggededrl pfrcdgcgla vdslhslllh rnvdcsrgaa pgggagtpss
      121 wrsssgsped eltsaslspp psllaggpas asdselsfti gvteatpyac hfcdkafprl
      181 sylkrheqvh sdrmpfrcdf cqrlfkhkrs rdrhvklhtg drkyrcsqce aafsrsdhlk
      241 ihmkthdhak pfqcamcnrg yntaaaltsh mqnhrksaaa vtnglepsaa lsrpgsslas
      301 angvlavltn padggstsms rddssscstd klsltggspg pgsvdvqang cksmserpgs
      361 sspreeplac gfcsrrdfgs lealqahvra shvpfsaasl vgpshggplr pepsgraasr
      421 hsppaattaa slvdasyece ycgmklpsvh alqthtlaah sfsevlskrv svmmgadsgg
      481 ggpllcsqcg aaspdfesfr ahlashlegr msrccpecra efpssqqlea hvaahflats
      541 veygcqaclk lfprpdelqk hlldvhahhl yrcalcrdvf dskvgvqvhf avkhsnecqv
      601 fkcsacnaay rsepefalhv qvthlakaap yrcllcdqtf asepllqshl dthgkqfaca
      661 lcsqafhvef llekhmqach aadltlhpdg vqnlsvkprv kdakcdicdq afgtdallsa
      721 hrkqvhnvrs qksvsllcay cnepcksrsd lenhmkshta spskhkcnic demcpsattl
      781 aehklthckv vkgstcagcr evlrseeqff shvnshgggp mlplpcvvcr qtlmsqvelr
      841 mharfhvahc dkgslccvce rpldaasrii tgrqqerqsf mckdcfhakg vqdqgrcpec
      901 rikfesplel eqhvrsshgg
//
LOCUS       XP_077503009             856 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger protein Lobe isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503009
VERSION     XP_077503009.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646883.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..856
                     /product="zinc finger protein Lobe isoform X2"
                     /calculated_mol_wt=92321
     Region          <66..184
                     /region_name="SFP1"
                     /note="Putative transcriptional repressor regulating G2/M
                     transition [Transcription / Cell division and chromosome
                     partitioning]; COG5189"
                     /db_xref="CDD:227516"
     Region          106..126
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(106,109,122,126)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(111,113,115,117..118,121..122,125,139,141,145..146,
                     149..150,153,167,169,171,173..174,177..178,181)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          134..154
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          157..592
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          162..>222
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          162..187
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          162..182
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(167,173,180,186,189,196)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          190..210
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          190..210
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          422..442
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(422,425,438,442)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(427,429,431,433..434,437..438,441,456,458,462..463,
                     466..467,470,486,488,490,492..493,496..497)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          450..471
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          481..498
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          539..560
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(539,542,555,560)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(544,546,548,550..551,554..555,559,574,576,580..581,
                     584..585,588,600,602,604,606..607,610..611)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          569..589
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          595..612
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          641..662
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(641,644,657,662)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(646,648,650,652..653,656..657,661,679,681,685..686,
                     689..690,693,708,710,712,714..715,718..719,722)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          674..694
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          703..723
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..856
                     /gene="L"
                     /coded_by="XM_077646883.1:137..2707"
                     /db_xref="GeneID:144113667"
ORIGIN      
        1 mcremaasdp gsdvdvdgte eldvgsesqp qemlaaaqgl arlgaapggg agtpsswrss
       61 sgspedelts aslspppsll aggpasasds elsftigvte atpyachfcd kafprlsylk
      121 rheqvhsdrm pfrcdfcqrl fkhkrsrdrh vklhtgdrky rcsqceaafs rsdhlkihmk
      181 thdhakpfqc amcnrgynta aaltshmqnh rksaaavtng lepsaalsrp gsslasangv
      241 lavltnpadg gstsmsrdds sscstdklsl tggspgpgsv dvqangcksm serpgssspr
      301 eeplacgfcs rrdfgsleal qahvrashvp fsaaslvgps hggplrpeps graasrhspp
      361 aattaaslvd asyeceycgm klpsvhalqt htlaahsfse vlskrvsvmm gadsggggpl
      421 lcsqcgaasp dfesfrahla shlegrmsrc cpecraefps sqqleahvaa hflatsveyg
      481 cqaclklfpr pdelqkhlld vhahhlyrca lcrdvfdskv gvqvhfavkh snecqvfkcs
      541 acnaayrsep efalhvqvth lakaapyrcl lcdqtfasep llqshldthg kqfacalcsq
      601 afhvefllek hmqachaadl tlhpdgvqnl svkprvkdak cdicdqafgt dallsahrkq
      661 vhnvrsqksv sllcaycnep cksrsdlenh mkshtaspsk hkcnicdemc psattlaehk
      721 lthckvvkgs tcagcrevlr seeqffshvn shgggpmlpl pcvvcrqtlm sqvelrmhar
      781 fhvahcdkgs lccvcerpld aasriitgrq qerqsfmckd cfhakgvqdq grcpecrikf
      841 espleleqhv rsshgg
//
LOCUS       XP_077503010             891 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger protein Lobe isoform X3 [Amblyomma americanum].
ACCESSION   XP_077503010
VERSION     XP_077503010.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646884.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..891
                     /product="zinc finger protein Lobe isoform X3"
                     /calculated_mol_wt=96379
     Region          <101..219
                     /region_name="SFP1"
                     /note="Putative transcriptional repressor regulating G2/M
                     transition [Transcription / Cell division and chromosome
                     partitioning]; COG5189"
                     /db_xref="CDD:227516"
     Region          141..161
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(141,144,157,161)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(146,148,150,152..153,156..157,160,174,176,180..181,
                     184..185,188,202,204,206,208..209,212..213,216)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          169..189
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          192..627
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          197..217
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          225..245
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          457..477
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(457,460,473,477)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(462,464,466,468..469,472..473,476,491,493,497..498,
                     501..502,505,521,523,525,527..528,531..532)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          485..506
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          516..533
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          574..595
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(574,577,590,595)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(579,581,583,585..586,589..590,594,609,611,615..616,
                     619..620,623,635,637,639,641..642,645..646)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          604..624
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          630..647
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          676..697
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(676,679,692,697)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(681,683,685,687..688,691..692,696,714,716,720..721,
                     724..725,728,743,745,747,749..750,753..754,757)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          709..729
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          738..758
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..891
                     /gene="L"
                     /coded_by="XM_077646884.1:106..2781"
                     /db_xref="GeneID:144113667"
ORIGIN      
        1 mcapaalsaa atfrckgqsi fdrgrqcnts rcgrarlwrp grlarammpa gaaatwpigp
       61 lcwaalepll wswtlpqhga apgggagtps swrsssgspe deltsaslsp ppsllaggpa
      121 sasdselsft igvteatpya chfcdkafpr lsylkrheqv hsdrmpfrcd fcqrlfkhkr
      181 srdrhvklht gdrkyrcsqc eaafsrsdhl kihmkthdha kpfqcamcnr gyntaaalts
      241 hmqnhrksaa avtnglepsa alsrpgssla sangvlavlt npadggstsm srddssscst
      301 dklsltggsp gpgsvdvqan gcksmserpg ssspreepla cgfcsrrdfg slealqahvr
      361 ashvpfsaas lvgpshggpl rpepsgraas rhsppaatta aslvdasyec eycgmklpsv
      421 halqthtlaa hsfsevlskr vsvmmgadsg gggpllcsqc gaaspdfesf rahlashleg
      481 rmsrccpecr aefpssqqle ahvaahflat sveygcqacl klfprpdelq khlldvhahh
      541 lyrcalcrdv fdskvgvqvh favkhsnecq vfkcsacnaa yrsepefalh vqvthlakaa
      601 pyrcllcdqt fasepllqsh ldthgkqfac alcsqafhve fllekhmqac haadltlhpd
      661 gvqnlsvkpr vkdakcdicd qafgtdalls ahrkqvhnvr sqksvsllca ycnepcksrs
      721 dlenhmksht aspskhkcni cdemcpsatt laehklthck vvkgstcagc revlrseeqf
      781 fshvnshggg pmlplpcvvc rqtlmsqvel rmharfhvah cdkgslccvc erpldaasri
      841 itgrqqerqs fmckdcfhak gvqdqgrcpe crikfesple leqhvrsshg g
//
LOCUS       XP_077503011             763 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger protein Lobe isoform X4 [Amblyomma americanum].
ACCESSION   XP_077503011
VERSION     XP_077503011.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646885.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..763
                     /product="zinc finger protein Lobe isoform X4"
                     /calculated_mol_wt=83309
     Region          41..61
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(41,44,57,61)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(46,48,50,52..53,56..57,60,74,76,80..81,84..85,88,
                     102,104,106,108..109,112..113,116)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          69..>129
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          69..94
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          69..89
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(74,80,87,93,96,103)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          97..117
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          97..117
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          329..349
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(329,332,345,349)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(334,336,338,340..341,344..345,348,363,365,369..370,
                     373..374,377,393,395,397,399..400,403..404)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          357..378
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          388..405
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          446..467
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(446,449,462,467)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(451,453,455,457..458,461..462,466,481,483,487..488,
                     491..492,495,507,509,511,513..514,517..518)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          476..496
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          502..519
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          548..569
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(548,551,564,569)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(553,555,557,559..560,563..564,568,586,588,592..593,
                     596..597,600,615,617,619,621..622,625..626,629)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          581..601
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          610..630
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..763
                     /gene="L"
                     /coded_by="XM_077646885.1:105..2396"
                     /db_xref="GeneID:144113667"
ORIGIN      
        1 mwtltaprss twdpsrsrrr cspprrdsld svhsdrmpfr cdfcqrlfkh krsrdrhvkl
       61 htgdrkyrcs qceaafsrsd hlkihmkthd hakpfqcamc nrgyntaaal tshmqnhrks
      121 aaavtnglep saalsrpgss lasangvlav ltnpadggst smsrddsssc stdklsltgg
      181 spgpgsvdvq angcksmser pgssspreep lacgfcsrrd fgslealqah vrashvpfsa
      241 aslvgpshgg plrpepsgra asrhsppaat taaslvdasy eceycgmklp svhalqthtl
      301 aahsfsevls krvsvmmgad sggggpllcs qcgaaspdfe sfrahlashl egrmsrccpe
      361 craefpssqq leahvaahfl atsveygcqa clklfprpde lqkhlldvha hhlyrcalcr
      421 dvfdskvgvq vhfavkhsne cqvfkcsacn aayrsepefa lhvqvthlak aapyrcllcd
      481 qtfasepllq shldthgkqf acalcsqafh vefllekhmq achaadltlh pdgvqnlsvk
      541 prvkdakcdi cdqafgtdal lsahrkqvhn vrsqksvsll caycnepcks rsdlenhmks
      601 htaspskhkc nicdemcpsa ttlaehklth ckvvkgstca gcrevlrsee qffshvnshg
      661 ggpmlplpcv vcrqtlmsqv elrmharfhv ahcdkgslcc vcerpldaas riitgrqqer
      721 qsfmckdcfh akgvqdqgrc pecrikfesp leleqhvrss hgg
//
LOCUS       XP_077503012             548 aa            linear   INV 25-NOV-2025
DEFINITION  cell division cycle protein 45 [Amblyomma americanum].
ACCESSION   XP_077503012
VERSION     XP_077503012.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646886.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..548
                     /product="cell division cycle protein 45"
                     /calculated_mol_wt=62012
     Region          20..543
                     /region_name="CDC45"
                     /note="CDC45-like protein; pfam02724"
                     /db_xref="CDD:460663"
     CDS             1..548
                     /gene="Cdc45"
                     /coded_by="XM_077646886.1:196..1842"
                     /db_xref="GeneID:144113668"
ORIGIN      
        1 myvsdlptdf yleviksral viiatdvdav aacrilqqlf hsdnalytlv pvskktavaa
       61 ayrdhgegik nvillncgat wdiieeckpe dddvifyiad snrpvhihni yntsqvrllm
      121 pkcaeegvpd yddlfredee eddeddmsie evadkrrerr qweerrkkll feytefsyhg
      181 qaaavtmfev ssrmdrdtpe ilwwaivgqs eqyiagkieh nryvleagdl qarvshqmnn
      241 iaarldplas navqisfdqe lalplythws lmeslrnspn ifckfklwtq kgnrklqefl
      301 aelglpllqc kqqyasmdis lknnvkewmc nmaekygldn llfacftgks gyrdhffasd
      361 tayglmalle spvedvttsf fstldalsws ntellrhgiq lakeclvatm qqvhsfmdlg
      421 siicagpfly gtvqegaqhs rhfgrpsalf rlaqcalqay aantksrrfa clplvlaady
      481 addgaytlvv gipplsedst knffgrafeq astmthctyq adffcspavl vykqdrgkfl
      541 dalvsllv
//
LOCUS       XP_077503013             594 aa            linear   INV 25-NOV-2025
DEFINITION  nudC domain-containing protein 1 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503013
VERSION     XP_077503013.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646887.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..594
                     /product="nudC domain-containing protein 1 isoform X1"
                     /calculated_mol_wt=66668
     Region          288..368
                     /region_name="alpha-crystallin-Hsps_p23-like"
                     /note="alpha-crystallin domain (ACD) found in
                     alpha-crystallin-type small heat shock proteins, and a
                     similar domain found in p23 (a cochaperone for Hsp90) and
                     in other p23-like proteins; cl00175"
                     /db_xref="CDD:469641"
     CDS             1..594
                     /gene="LOC144113669"
                     /coded_by="XM_077646887.1:38..1822"
                     /db_xref="GeneID:144113669"
ORIGIN      
        1 maasvaklhl ipnrnlldak fesyrlslep lptirqsfqg gvqkvhledd qygylhvqms
       61 gfinalvvdp wdantlffms eahhvmwthf sqetrllepr vvwkipeasg acrtgtldfp
      121 haewclisdg mgtiyilnsp srpqgepwql cythrlewsr pgtilltsah snfpssqqme
      181 cllayisrpq dvqgsvsipe ttvhflcvle wltftcmsng egglwsvkrh rrlvgasipk
      241 yvsfdthgna lclaseksfs fvydsckaev gheveetkgg kpqdpplyyf iqtaedirvf
      301 frlneaspkq pkakidgkti tvtidgemvl rgefphtvks dssfstqgdk leirllkaer
      361 gvvwhevvvg dsrgeevidp afveeiharl ahltsdseqv agsekpsfny qeldecdntt
      421 eyftftrlcg dshrathqin lsghqwlfsv rssadlmpal clrhdvdglv wqprnvmaeg
      481 dadsfrvehv atfnafgyvq askqdrkfva aspdfsfaav cnavrhvyly rqpetignsq
      541 elrnrktgre vstiakqhvi sldqcdsilg mvtnrrcifv lspgalyavk vpss
//
LOCUS       XP_077503014             593 aa            linear   INV 25-NOV-2025
DEFINITION  nudC domain-containing protein 1 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503014
VERSION     XP_077503014.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646888.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..593
                     /product="nudC domain-containing protein 1 isoform X2"
                     /calculated_mol_wt=66540
     Region          288..368
                     /region_name="alpha-crystallin-Hsps_p23-like"
                     /note="alpha-crystallin domain (ACD) found in
                     alpha-crystallin-type small heat shock proteins, and a
                     similar domain found in p23 (a cochaperone for Hsp90) and
                     in other p23-like proteins; cl00175"
                     /db_xref="CDD:469641"
     CDS             1..593
                     /gene="LOC144113669"
                     /coded_by="XM_077646888.1:38..1819"
                     /db_xref="GeneID:144113669"
ORIGIN      
        1 maasvaklhl ipnrnlldak fesyrlslep lptirqsfqg gvqkvhledd qygylhvqms
       61 gfinalvvdp wdantlffms eahhvmwthf sqetrllepr vvwkipeasg acrtgtldfp
      121 haewclisdg mgtiyilnsp srpqgepwql cythrlewsr pgtilltsah snfpssqqme
      181 cllayisrpq dvqgsvsipe ttvhflcvle wltftcmsng egglwsvkrh rrlvgasipk
      241 yvsfdthgna lclaseksfs fvydsckaev gheveetkgg kpqdpplyyf iqtaedirvf
      301 frlneaspkq pkakidgkti tvtidgemvl rgefphtvks dssfstqgdk leirllkaer
      361 gvvwhevvvg dsrgeevidp afveeiharl ahltsdseva gsekpsfnyq eldecdntte
      421 yftftrlcgd shrathqinl sghqwlfsvr ssadlmpalc lrhdvdglvw qprnvmaegd
      481 adsfrvehva tfnafgyvqa skqdrkfvaa spdfsfaavc navrhvylyr qpetignsqe
      541 lrnrktgrev stiakqhvis ldqcdsilgm vtnrrcifvl spgalyavkv pss
//
LOCUS       XP_077503015             590 aa            linear   INV 25-NOV-2025
DEFINITION  organic cation transporter protein-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503015
VERSION     XP_077503015.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646889.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..590
                     /product="organic cation transporter protein-like isoform
                     X1"
                     /calculated_mol_wt=64945
     Region          35..552
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..590
                     /gene="LOC144113670"
                     /coded_by="XM_077646889.1:2549..4321"
                     /db_xref="GeneID:144113670"
ORIGIN      
        1 msltwrslla psslhvtphc eapamdldel lpeigafgpy qkwiyfllci patlpavftl
       61 fssifvsatp rhrcrvpele alrhafspae laelsiprra gggefdgcrm ydlnytavvl
      121 rvldegslpr pnaswltrac dhgwvydtty yddtlvtqmn vvcddawkvs maqcsfyigs
      181 vagnllfgyi gdrwgrkksf fltivvnvvg gigaafpaty eayiawrgii gmvypaifqt
      241 pfimsmeims pekrtfagmf vcavygvalm llagvayllr swfavslats lpfvallgfw
      301 wlvpesprwl lsqhklaeae vivqkiakrn gkkippnflk tfmdqkqsla rrdsavegev
      361 slrrlvqypn llkkvvvvsf swtantmvyn glsfavssmg isdyvsftis gaaevpgvcl
      421 awyfmdrhgr rpvmvfsmll gglaciatvf aasdsawptv mlcslgkmsi tasfatiyvy
      481 saelfptvlr tfamgvaamt asisliitph ilylgqlygv vipsaifgvl svaggvavll
      541 lpetmkvrlp etlsegeafg kdvrfcsctg rrasnedtem idqqqetlrv
//
LOCUS       XP_077503016             566 aa            linear   INV 25-NOV-2025
DEFINITION  organic cation transporter protein-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503016
VERSION     XP_077503016.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646890.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..566
                     /product="organic cation transporter protein-like isoform
                     X2"
                     /calculated_mol_wt=62358
     Region          11..528
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..566
                     /gene="LOC144113670"
                     /coded_by="XM_077646890.1:76..1776"
                     /db_xref="GeneID:144113670"
ORIGIN      
        1 mdldellpei gafgpyqkwi yfllcipatl pavftlfssi fvsatprhrc rvpelealrh
       61 afspaelael siprraggge fdgcrmydln ytavvlrvld egslprpnas wltracdhgw
      121 vydttyyddt lvtqmnvvcd dawkvsmaqc sfyigsvagn llfgyigdrw grkksfflti
      181 vvnvvggiga afpatyeayi awrgiigmvy paifqtpfim smeimspekr tfagmfvcav
      241 ygvalmllag vayllrswfa vslatslpfv allgfwwlvp esprwllsqh klaeaevivq
      301 kiakrngkki ppnflktfmd qkqslarrds avegevslrr lvqypnllkk vvvvsfswta
      361 ntmvynglsf avssmgisdy vsftisgaae vpgvclawyf mdrhgrrpvm vfsmllggla
      421 ciatvfaasd sawptvmlcs lgkmsitasf atiyvysael fptvlrtfam gvaamtasis
      481 liitphilyl gqlygvvips aifgvlsvag gvavlllpet mkvrlpetls egeafgkdvr
      541 fcsctgrras nedtemidqq qetlrv
//
LOCUS       XP_077503017             566 aa            linear   INV 25-NOV-2025
DEFINITION  organic cation transporter protein-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503017
VERSION     XP_077503017.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646891.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..566
                     /product="organic cation transporter protein-like isoform
                     X2"
                     /calculated_mol_wt=62358
     Region          11..528
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..566
                     /gene="LOC144113670"
                     /coded_by="XM_077646891.1:164..1864"
                     /db_xref="GeneID:144113670"
ORIGIN      
        1 mdldellpei gafgpyqkwi yfllcipatl pavftlfssi fvsatprhrc rvpelealrh
       61 afspaelael siprraggge fdgcrmydln ytavvlrvld egslprpnas wltracdhgw
      121 vydttyyddt lvtqmnvvcd dawkvsmaqc sfyigsvagn llfgyigdrw grkksfflti
      181 vvnvvggiga afpatyeayi awrgiigmvy paifqtpfim smeimspekr tfagmfvcav
      241 ygvalmllag vayllrswfa vslatslpfv allgfwwlvp esprwllsqh klaeaevivq
      301 kiakrngkki ppnflktfmd qkqslarrds avegevslrr lvqypnllkk vvvvsfswta
      361 ntmvynglsf avssmgisdy vsftisgaae vpgvclawyf mdrhgrrpvm vfsmllggla
      421 ciatvfaasd sawptvmlcs lgkmsitasf atiyvysael fptvlrtfam gvaamtasis
      481 liitphilyl gqlygvvips aifgvlsvag gvavlllpet mkvrlpetls egeafgkdvr
      541 fcsctgrras nedtemidqq qetlrv
//
LOCUS       XP_077503018             566 aa            linear   INV 25-NOV-2025
DEFINITION  organic cation transporter protein-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503018
VERSION     XP_077503018.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646892.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..566
                     /product="organic cation transporter protein-like isoform
                     X2"
                     /calculated_mol_wt=62358
     Region          11..528
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..566
                     /gene="LOC144113670"
                     /coded_by="XM_077646892.1:39..1739"
                     /db_xref="GeneID:144113670"
ORIGIN      
        1 mdldellpei gafgpyqkwi yfllcipatl pavftlfssi fvsatprhrc rvpelealrh
       61 afspaelael siprraggge fdgcrmydln ytavvlrvld egslprpnas wltracdhgw
      121 vydttyyddt lvtqmnvvcd dawkvsmaqc sfyigsvagn llfgyigdrw grkksfflti
      181 vvnvvggiga afpatyeayi awrgiigmvy paifqtpfim smeimspekr tfagmfvcav
      241 ygvalmllag vayllrswfa vslatslpfv allgfwwlvp esprwllsqh klaeaevivq
      301 kiakrngkki ppnflktfmd qkqslarrds avegevslrr lvqypnllkk vvvvsfswta
      361 ntmvynglsf avssmgisdy vsftisgaae vpgvclawyf mdrhgrrpvm vfsmllggla
      421 ciatvfaasd sawptvmlcs lgkmsitasf atiyvysael fptvlrtfam gvaamtasis
      481 liitphilyl gqlygvvips aifgvlsvag gvavlllpet mkvrlpetls egeafgkdvr
      541 fcsctgrras nedtemidqq qetlrv
//
LOCUS       XP_077503019             566 aa            linear   INV 25-NOV-2025
DEFINITION  organic cation transporter protein-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503019
VERSION     XP_077503019.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646893.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..566
                     /product="organic cation transporter protein-like isoform
                     X2"
                     /calculated_mol_wt=62358
     Region          11..528
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..566
                     /gene="LOC144113670"
                     /coded_by="XM_077646893.1:35..1735"
                     /db_xref="GeneID:144113670"
ORIGIN      
        1 mdldellpei gafgpyqkwi yfllcipatl pavftlfssi fvsatprhrc rvpelealrh
       61 afspaelael siprraggge fdgcrmydln ytavvlrvld egslprpnas wltracdhgw
      121 vydttyyddt lvtqmnvvcd dawkvsmaqc sfyigsvagn llfgyigdrw grkksfflti
      181 vvnvvggiga afpatyeayi awrgiigmvy paifqtpfim smeimspekr tfagmfvcav
      241 ygvalmllag vayllrswfa vslatslpfv allgfwwlvp esprwllsqh klaeaevivq
      301 kiakrngkki ppnflktfmd qkqslarrds avegevslrr lvqypnllkk vvvvsfswta
      361 ntmvynglsf avssmgisdy vsftisgaae vpgvclawyf mdrhgrrpvm vfsmllggla
      421 ciatvfaasd sawptvmlcs lgkmsitasf atiyvysael fptvlrtfam gvaamtasis
      481 liitphilyl gqlygvvips aifgvlsvag gvavlllpet mkvrlpetls egeafgkdvr
      541 fcsctgrras nedtemidqq qetlrv
//
LOCUS       XP_077503020             566 aa            linear   INV 25-NOV-2025
DEFINITION  organic cation transporter protein-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503020
VERSION     XP_077503020.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646894.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..566
                     /product="organic cation transporter protein-like isoform
                     X2"
                     /calculated_mol_wt=62358
     Region          11..528
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..566
                     /gene="LOC144113670"
                     /coded_by="XM_077646894.1:157..1857"
                     /db_xref="GeneID:144113670"
ORIGIN      
        1 mdldellpei gafgpyqkwi yfllcipatl pavftlfssi fvsatprhrc rvpelealrh
       61 afspaelael siprraggge fdgcrmydln ytavvlrvld egslprpnas wltracdhgw
      121 vydttyyddt lvtqmnvvcd dawkvsmaqc sfyigsvagn llfgyigdrw grkksfflti
      181 vvnvvggiga afpatyeayi awrgiigmvy paifqtpfim smeimspekr tfagmfvcav
      241 ygvalmllag vayllrswfa vslatslpfv allgfwwlvp esprwllsqh klaeaevivq
      301 kiakrngkki ppnflktfmd qkqslarrds avegevslrr lvqypnllkk vvvvsfswta
      361 ntmvynglsf avssmgisdy vsftisgaae vpgvclawyf mdrhgrrpvm vfsmllggla
      421 ciatvfaasd sawptvmlcs lgkmsitasf atiyvysael fptvlrtfam gvaamtasis
      481 liitphilyl gqlygvvips aifgvlsvag gvavlllpet mkvrlpetls egeafgkdvr
      541 fcsctgrras nedtemidqq qetlrv
//
LOCUS       XP_077503021             566 aa            linear   INV 25-NOV-2025
DEFINITION  organic cation transporter protein-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503021
VERSION     XP_077503021.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646895.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..566
                     /product="organic cation transporter protein-like isoform
                     X2"
                     /calculated_mol_wt=62358
     Region          11..528
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..566
                     /gene="LOC144113670"
                     /coded_by="XM_077646895.1:23..1723"
                     /db_xref="GeneID:144113670"
ORIGIN      
        1 mdldellpei gafgpyqkwi yfllcipatl pavftlfssi fvsatprhrc rvpelealrh
       61 afspaelael siprraggge fdgcrmydln ytavvlrvld egslprpnas wltracdhgw
      121 vydttyyddt lvtqmnvvcd dawkvsmaqc sfyigsvagn llfgyigdrw grkksfflti
      181 vvnvvggiga afpatyeayi awrgiigmvy paifqtpfim smeimspekr tfagmfvcav
      241 ygvalmllag vayllrswfa vslatslpfv allgfwwlvp esprwllsqh klaeaevivq
      301 kiakrngkki ppnflktfmd qkqslarrds avegevslrr lvqypnllkk vvvvsfswta
      361 ntmvynglsf avssmgisdy vsftisgaae vpgvclawyf mdrhgrrpvm vfsmllggla
      421 ciatvfaasd sawptvmlcs lgkmsitasf atiyvysael fptvlrtfam gvaamtasis
      481 liitphilyl gqlygvvips aifgvlsvag gvavlllpet mkvrlpetls egeafgkdvr
      541 fcsctgrras nedtemidqq qetlrv
//
LOCUS       XP_077503022             461 aa            linear   INV 25-NOV-2025
DEFINITION  beta-alanine transporter-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077503022
VERSION     XP_077503022.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646896.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..461
                     /product="beta-alanine transporter-like isoform X3"
                     /calculated_mol_wt=51027
     Region          35..450
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..461
                     /gene="LOC144113670"
                     /coded_by="XM_077646896.1:2561..3946"
                     /db_xref="GeneID:144113670"
ORIGIN      
        1 msltwrslla psslhvtphc eapamdldel lpeigafgpy qkwiyfllci patlpavftl
       61 fssifvsatp rhrcrvpele alrhafspae laelsiprra gggefdgcrm ydlnytavvl
      121 rvldegslpr pnaswltrac dhgwvydtty yddtlvtqmn vvcddawkvs maqcsfyigs
      181 vagnllfgyi gdrwgrkksf fltivvnvvg gigaafpaty eayiawrgii gmvypaifqt
      241 pfimsmeims pekrtfagmf vcavygvalm llagvayllr swfavslats lpfvallgfw
      301 wlvpesprwl lsqhklaeae vivqkiakrn gkkippnflk tfmdqkqsla rrdsavegev
      361 slrrlvqypn llkkvvvvsf swtantmvyn glsfavssmg isdyvsftis gaaevpgvcl
      421 awyfmdrhgr rpvmvfsmll gglaciatvf aasaqivrgp p
//
LOCUS       XP_077503023             137 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113671 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503023
VERSION     XP_077503023.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646897.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..137
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..137
                     /product="uncharacterized protein LOC144113671 isoform X1"
                     /calculated_mol_wt=15320
     CDS             1..137
                     /gene="LOC144113671"
                     /coded_by="XM_077646897.1:90..503"
                     /db_xref="GeneID:144113671"
ORIGIN      
        1 mavhlnplra rpwsrlllwr grqrapkptl fptaagsprt rresfrakct arrgglgaat
       61 ererrsrfvq aartmpeksn sggykddrwk vkaipfppsa errdtgfrey hpgvlhprsg
      121 gsspttpqps qggwkys
//
LOCUS       XP_077503024              63 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113671 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503024
VERSION     XP_077503024.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646898.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..63
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..63
                     /product="uncharacterized protein LOC144113671 isoform X2"
                     /calculated_mol_wt=6871
     CDS             1..63
                     /gene="LOC144113671"
                     /coded_by="XM_077646898.1:308..499"
                     /db_xref="GeneID:144113671"
ORIGIN      
        1 mpeksnsggy kddrwkvkai pfppsaerrd tgfreyhpgv lhprsggssp ttpqpsqggw
       61 kys
//
LOCUS       XP_077503025              63 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113671 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503025
VERSION     XP_077503025.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646899.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..63
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..63
                     /product="uncharacterized protein LOC144113671 isoform X2"
                     /calculated_mol_wt=6871
     CDS             1..63
                     /gene="LOC144113671"
                     /coded_by="XM_077646899.1:420..611"
                     /db_xref="GeneID:144113671"
ORIGIN      
        1 mpeksnsggy kddrwkvkai pfppsaerrd tgfreyhpgv lhprsggssp ttpqpsqggw
       61 kys
//
LOCUS       XP_077503026              63 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113671 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503026
VERSION     XP_077503026.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646900.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..63
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..63
                     /product="uncharacterized protein LOC144113671 isoform X2"
                     /calculated_mol_wt=6871
     CDS             1..63
                     /gene="LOC144113671"
                     /coded_by="XM_077646900.1:261..452"
                     /db_xref="GeneID:144113671"
ORIGIN      
        1 mpeksnsggy kddrwkvkai pfppsaerrd tgfreyhpgv lhprsggssp ttpqpsqggw
       61 kys
//
LOCUS       XP_077503027              63 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113671 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503027
VERSION     XP_077503027.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646901.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..63
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..63
                     /product="uncharacterized protein LOC144113671 isoform X2"
                     /calculated_mol_wt=6871
     CDS             1..63
                     /gene="LOC144113671"
                     /coded_by="XM_077646901.1:266..457"
                     /db_xref="GeneID:144113671"
ORIGIN      
        1 mpeksnsggy kddrwkvkai pfppsaerrd tgfreyhpgv lhprsggssp ttpqpsqggw
       61 kys
//
LOCUS       XP_077503028            1129 aa            linear   INV 25-NOV-2025
DEFINITION  retinoblastoma-like protein 1 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503028
VERSION     XP_077503028.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646902.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1129
                     /product="retinoblastoma-like protein 1 isoform X1"
                     /calculated_mol_wt=126187
     Region          73..209
                     /region_name="DUF3452"
                     /note="Domain of unknown function (DUF3452); pfam11934"
                     /db_xref="CDD:463402"
     Region          380..576
                     /region_name="RB_A"
                     /note="Retinoblastoma-associated protein A domain;
                     pfam01858"
                     /db_xref="CDD:460364"
     Region          815..1007
                     /region_name="CYCLIN_SF"
                     /note="Cyclin box fold superfamily; cl40454"
                     /db_xref="CDD:477360"
     CDS             1..1129
                     /gene="LOC144113673"
                     /coded_by="XM_077646902.1:147..3536"
                     /db_xref="GeneID:144113673"
ORIGIN      
        1 mglssegdqe irarfqevcs slnmdrltsd qalntyesis mnytlegntl hwiacalyve
       61 crsgnvptva qtsriegncv sltrllttcs isllefftkm kkwadmanln ermrqrieti
      121 eksfcvtaki yekyaqifat vfkenqggaq rrcikpakrr vhrpaqcnsn dmfhfiwtly
      181 vyikgrfsai ccdlvncyhl llccidygyc talankrtdl lspafyeahq eklkdsdnqd
      241 vgiirelvks hdgdfedasv lkahyfrypi qqlmeekill gdlktlalsl dptafdssmk
      301 klnktyeegv lkegelderi flddnaqeri gtprhtldsv qrelrmshsd tvvklvevpq
      361 lltpltgrsn lhnreainrs pvstatqcas klqallagck napskelqdl fstfdpslea
      421 siletvktmg dtfcsayaqp vsdqrsssah mdfarkrlql getlfykale nivnievrqh
      481 kpntdltahl shsvflqslf accleivmfc ynsqrefpwi levfnlmpyn fykiieplir
      541 aeeslwrevv khlnhieeqi leclawkdds plwdaiehse qsvpackevl lqrqietfqe
      601 sdssdvseaq spvphlrglk gdqdgsqssr kacmadvtlp selcegaicq psdpggrfsv
      661 gagraqcvaa pclcgfdmva fdadvalspv svaerfqspg savrrrlfap nitgggasas
      721 lqqspraesg tmgqartvsi tvqplqgsct trytpvdgkg aapatdstrp nivtlstapa
      781 papapapapv papvstpvpa pvpenpkekp lrsgslalff rkvyhlagvr fqdicarlgi
      841 tqkdiqlkiw tcfehsivcq tdlmrdrhld qlilcsvyvi tkvtrvdksf qeilhcyrqq
      901 pqnkshvyrs vllrknpths dnssrtgsns sspvpvered gdnkrtehls strssstlpl
      961 tlpnsqpptp trlagtatqf efdergdiik fyneiyrkrm tsfaikfspd ssknenvglt
     1021 plprakcmvn sprrqvshnr kvfvsplkag cypvspkhht yhfsqspakd lrvinrlvss
     1081 sdsvskrilh eddnsedesp atkrcffkki qtvmkerqdv cgggsssdn
//
LOCUS       XP_077503030            1077 aa            linear   INV 25-NOV-2025
DEFINITION  retinoblastoma-like protein 2 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503030
VERSION     XP_077503030.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646904.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1077
                     /product="retinoblastoma-like protein 2 isoform X2"
                     /calculated_mol_wt=120995
     Region          73..209
                     /region_name="DUF3452"
                     /note="Domain of unknown function (DUF3452); pfam11934"
                     /db_xref="CDD:463402"
     Region          380..576
                     /region_name="RB_A"
                     /note="Retinoblastoma-associated protein A domain;
                     pfam01858"
                     /db_xref="CDD:460364"
     Region          763..955
                     /region_name="CYCLIN_SF"
                     /note="Cyclin box fold superfamily; cl40454"
                     /db_xref="CDD:477360"
     CDS             1..1077
                     /gene="LOC144113673"
                     /coded_by="XM_077646904.1:124..3357"
                     /db_xref="GeneID:144113673"
ORIGIN      
        1 mglssegdqe irarfqevcs slnmdrltsd qalntyesis mnytlegntl hwiacalyve
       61 crsgnvptva qtsriegncv sltrllttcs isllefftkm kkwadmanln ermrqrieti
      121 eksfcvtaki yekyaqifat vfkenqggaq rrcikpakrr vhrpaqcnsn dmfhfiwtly
      181 vyikgrfsai ccdlvncyhl llccidygyc talankrtdl lspafyeahq eklkdsdnqd
      241 vgiirelvks hdgdfedasv lkahyfrypi qqlmeekill gdlktlalsl dptafdssmk
      301 klnktyeegv lkegelderi flddnaqeri gtprhtldsv qrelrmshsd tvvklvevpq
      361 lltpltgrsn lhnreainrs pvstatqcas klqallagck napskelqdl fstfdpslea
      421 siletvktmg dtfcsayaqp vsdqrsssah mdfarkrlql getlfykale nivnievrqh
      481 kpntdltahl shsvflqslf accleivmfc ynsqrefpwi levfnlmpyn fykiieplir
      541 aeeslwrevv khlnhieeqi leclawkdds plwdaiehse qsvpackevl lqrqietfqe
      601 sdssdvseaq spvphlrglk gdqdgsqssr kdvalspvsv aerfqspgsa vrrrlfapni
      661 tgggasaslq qspraesgtm gqartvsitv qplqgscttr ytpvdgkgaa patdstrpni
      721 vtlstapapa papapapvpa pvstpvpapv penpkekplr sgslalffrk vyhlagvrfq
      781 dicarlgitq kdiqlkiwtc fehsivcqtd lmrdrhldql ilcsvyvitk vtrvdksfqe
      841 ilhcyrqqpq nkshvyrsvl lrknpthsdn ssrtgsnsss pvpveredgd nkrtehlsst
      901 rssstlpltl pnsqpptptr lagtatqfef dergdiikfy neiyrkrmts faikfspdss
      961 knenvgltpl prakcmvnsp rrqvshnrkv fvsplkagcy pvspkhhtyh fsqspakdlr
     1021 vinrlvsssd svskrilhed dnsedespat krcffkkiqt vmkerqdvcg ggsssdn
//
LOCUS       XP_077503031            1017 aa            linear   INV 25-NOV-2025
DEFINITION  retinoblastoma-like protein 2 isoform X3 [Amblyomma americanum].
ACCESSION   XP_077503031
VERSION     XP_077503031.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646905.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1017
                     /product="retinoblastoma-like protein 2 isoform X3"
                     /calculated_mol_wt=114830
     Region          73..209
                     /region_name="DUF3452"
                     /note="Domain of unknown function (DUF3452); pfam11934"
                     /db_xref="CDD:463402"
     Region          380..576
                     /region_name="RB_A"
                     /note="Retinoblastoma-associated protein A domain;
                     pfam01858"
                     /db_xref="CDD:460364"
     Region          703..895
                     /region_name="CYCLIN_SF"
                     /note="Cyclin box fold superfamily; cl40454"
                     /db_xref="CDD:477360"
     CDS             1..1017
                     /gene="LOC144113673"
                     /coded_by="XM_077646905.1:404..3457"
                     /db_xref="GeneID:144113673"
ORIGIN      
        1 mglssegdqe irarfqevcs slnmdrltsd qalntyesis mnytlegntl hwiacalyve
       61 crsgnvptva qtsriegncv sltrllttcs isllefftkm kkwadmanln ermrqrieti
      121 eksfcvtaki yekyaqifat vfkenqggaq rrcikpakrr vhrpaqcnsn dmfhfiwtly
      181 vyikgrfsai ccdlvncyhl llccidygyc talankrtdl lspafyeahq eklkdsdnqd
      241 vgiirelvks hdgdfedasv lkahyfrypi qqlmeekill gdlktlalsl dptafdssmk
      301 klnktyeegv lkegelderi flddnaqeri gtprhtldsv qrelrmshsd tvvklvevpq
      361 lltpltgrsn lhnreainrs pvstatqcas klqallagck napskelqdl fstfdpslea
      421 siletvktmg dtfcsayaqp vsdqrsssah mdfarkrlql getlfykale nivnievrqh
      481 kpntdltahl shsvflqslf accleivmfc ynsqrefpwi levfnlmpyn fykiieplir
      541 aeeslwrevv khlnhieeqi leclawkdds plwdaiehse qsvpackevl lqrqietfqe
      601 sdssdvseaq spvphlrglk gdqdgsqssr kalqgscttr ytpvdgkgaa patdstrpni
      661 vtlstapapa papapapvpa pvstpvpapv penpkekplr sgslalffrk vyhlagvrfq
      721 dicarlgitq kdiqlkiwtc fehsivcqtd lmrdrhldql ilcsvyvitk vtrvdksfqe
      781 ilhcyrqqpq nkshvyrsvl lrknpthsdn ssrtgsnsss pvpveredgd nkrtehlsst
      841 rssstlpltl pnsqpptptr lagtatqfef dergdiikfy neiyrkrmts faikfspdss
      901 knenvgltpl prakcmvnsp rrqvshnrkv fvsplkagcy pvspkhhtyh fsqspakdlr
      961 vinrlvsssd svskrilhed dnsedespat krcffkkiqt vmkerqdvcg ggsssdn
//
LOCUS       XP_077503032             857 aa            linear   INV 25-NOV-2025
DEFINITION  lon protease homolog 2, peroxisomal-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503032
VERSION     XP_077503032.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646906.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..857
                     /product="lon protease homolog 2, peroxisomal-like isoform
                     X1"
                     /calculated_mol_wt=94281
     Region          38..847
                     /region_name="Lon"
                     /note="ATP-dependent Lon protease, bacterial type
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG0466"
                     /db_xref="CDD:440234"
     CDS             1..857
                     /gene="LOC144113675"
                     /coded_by="XM_077646906.1:93..2666"
                     /db_xref="GeneID:144113675"
ORIGIN      
        1 mfevpaiscg gasvasvvsr matptarlpr rlplllidgv lfpgasirip vsshrnmnmv
       61 khhllghstf ssaivgvvpr edsesheeem wsmhhlgtag ivvqvtgtnw prpsytllvt
      121 glcrfridgl vqespylvgn vsqldklpge dgsmhiddcd telmelmgqf reqvnklidt
      181 ldlsvpyvik lkrflaslpi qslpdvcaai vrathaerlq vldavdlger fkktlpllvr
      241 qieglqffhq akldgstsrs fqiekvgsph rprqqldidd ddfedrdevg llekkikaae
      301 mpdharkvaa kelqrlkkmp phmpehamtr nylellidlp wshsssevvd laksrsdlda
      361 dhygmeklkk rvleylavrk lksslkgpil cfvgppgvgk tsvgrsiahs lgrefyrisl
      421 ggvsdqadir ghrrtyigsm pgriiqglrt vgvknpvfll deidkmtpgi hgdpaaalle
      481 vldpeqncaf idhylnipyd lssvlfiata ntvstipaal ldrmevipmp gytheekehi
      541 arnhllpkql kehgittdmm qvtrealkkl vvkytreagv ralerkigal cravavkive
      601 sealcpvtlq hdqlskqsav tvavarppsl pivideiaie tilgppqyeq dmdsrvlqpg
      661 vavglawtva ggeimfveas rmegdgqlvl tgqlgdvmke samlalnwlr ihadmfhlpv
      721 qkgtdlmegi dvhihfpaga vgkdgpsagi tivtvlvslf tgrtvlpdva mtgeitlqgl
      781 vlpvggikdk vlaahragmr kvilpkrnkk dlqevpesik kdlyfcfvth ieevlqeafe
      841 ggfpdlpssv vhtlskl
//
LOCUS       XP_077503033             801 aa            linear   INV 25-NOV-2025
DEFINITION  lon protease homolog 2, peroxisomal-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503033
VERSION     XP_077503033.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646907.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..801
                     /product="lon protease homolog 2, peroxisomal-like isoform
                     X2"
                     /calculated_mol_wt=88413
     Region          18..791
                     /region_name="Lon"
                     /note="ATP-dependent Lon protease, bacterial type
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG0466"
                     /db_xref="CDD:440234"
     CDS             1..801
                     /gene="LOC144113675"
                     /coded_by="XM_077646907.1:120..2525"
                     /db_xref="GeneID:144113675"
ORIGIN      
        1 mnmvkhhllg hstfssaivg vvpredsesh eeemwsmhhl gtagivvqvt gtnwprpsyt
       61 llvtglcrfr idglvqespy lvgnvsqldk lpgedgsmhi ddcdtelmel mgqfreqvnk
      121 lidtldlsvp yviklkrfla slpiqslpdv caaivratha erlqvldavd lgerfkktlp
      181 llvrqieglq ffhqakldgs tsrsfqiekv gsphrprqql diddddfedr devgllekki
      241 kaaempdhar kvaakelqrl kkmpphmpeh amtrnylell idlpwshsss evvdlaksrs
      301 dldadhygme klkkrvleyl avrklksslk gpilcfvgpp gvgktsvgrs iahslgrefy
      361 rislggvsdq adirghrrty igsmpgriiq glrtvgvknp vflldeidkm tpgihgdpaa
      421 allevldpeq ncafidhyln ipydlssvlf iatantvsti paalldrmev ipmpgythee
      481 kehiarnhll pkqlkehgit tdmmqvtrea lkklvvkytr eagvralerk igalcravav
      541 kivesealcp vtlqhdqlsk qsavtvavar ppslpivide iaietilgpp qyeqdmdsrv
      601 lqpgvavgla wtvaggeimf veasrmegdg qlvltgqlgd vmkesamlal nwlrihadmf
      661 hlpvqkgtdl megidvhihf pagavgkdgp sagitivtvl vslftgrtvl pdvamtgeit
      721 lqglvlpvgg ikdkvlaahr agmrkvilpk rnkkdlqevp esikkdlyfc fvthieevlq
      781 eafeggfpdl pssvvhtlsk l
//
LOCUS       XP_077503034             788 aa            linear   INV 25-NOV-2025
DEFINITION  lon protease homolog 2, peroxisomal-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503034
VERSION     XP_077503034.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646908.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..788
                     /product="lon protease homolog 2, peroxisomal-like isoform
                     X3"
                     /calculated_mol_wt=87181
     Region          24..778
                     /region_name="Lon"
                     /note="ATP-dependent Lon protease, bacterial type
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG0466"
                     /db_xref="CDD:440234"
     CDS             1..788
                     /gene="LOC144113675"
                     /coded_by="XM_077646908.1:227..2593"
                     /db_xref="GeneID:144113675"
ORIGIN      
        1 mrwvhnlesa apyrqknvee mwsmhhlgta givvqvtgtn wprpsytllv tglcrfridg
       61 lvqespylvg nvsqldklpg edgsmhiddc dtelmelmgq freqvnklid tldlsvpyvi
      121 klkrflaslp iqslpdvcaa ivrathaerl qvldavdlge rfkktlpllv rqieglqffh
      181 qakldgstsr sfqiekvgsp hrprqqldid dddfedrdev gllekkikaa empdharkva
      241 akelqrlkkm pphmpehamt rnylellidl pwshsssevv dlaksrsdld adhygmeklk
      301 krvleylavr klksslkgpi lcfvgppgvg ktsvgrsiah slgrefyris lggvsdqadi
      361 rghrrtyigs mpgriiqglr tvgvknpvfl ldeidkmtpg ihgdpaaall evldpeqnca
      421 fidhylnipy dlssvlfiat antvstipaa lldrmevipm pgytheekeh iarnhllpkq
      481 lkehgittdm mqvtrealkk lvvkytreag vralerkiga lcravavkiv esealcpvtl
      541 qhdqlskqsa vtvavarpps lpivideiai etilgppqye qdmdsrvlqp gvavglawtv
      601 aggeimfvea srmegdgqlv ltgqlgdvmk esamlalnwl rihadmfhlp vqkgtdlmeg
      661 idvhihfpag avgkdgpsag itivtvlvsl ftgrtvlpdv amtgeitlqg lvlpvggikd
      721 kvlaahragm rkvilpkrnk kdlqevpesi kkdlyfcfvt hieevlqeaf eggfpdlpss
      781 vvhtlskl
//
LOCUS       XP_077503035              99 aa            linear   INV 25-NOV-2025
DEFINITION  venom protein 302-like [Amblyomma americanum].
ACCESSION   XP_077503035
VERSION     XP_077503035.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646909.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..99
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..99
                     /product="venom protein 302-like"
                     /calculated_mol_wt=10236
     Region          25..77
                     /region_name="IGFBP"
                     /note="Insulin-like growth factor binding protein;
                     pfam00219"
                     /db_xref="CDD:459717"
     CDS             1..99
                     /gene="LOC144113676"
                     /coded_by="XM_077646909.1:269..568"
                     /db_xref="GeneID:144113676"
ORIGIN      
        1 mkttlcatll fvglvalaes mpscpdclev ecpleedcay gvtsdtcgcc kvcasgpgee
       61 cggylnhggt caegltcrpn ltyyqlpgkc vhmqldsts
//
LOCUS       XP_077503036             914 aa            linear   INV 25-NOV-2025
DEFINITION  protein phosphatase PHLPP-like protein [Amblyomma americanum].
ACCESSION   XP_077503036
VERSION     XP_077503036.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646910.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..914
                     /product="protein phosphatase PHLPP-like protein"
                     /calculated_mol_wt=99212
     Region          <51..281
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          51..70
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          73..95
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          96..118
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          119..141
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          142..164
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          165..211
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          212..235
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          236..274
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          275..297
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <299..>514
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          299..322
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          323..345
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          346..369
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          370..385
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          393..417
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          418..440
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          441..463
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          464..485
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          486..508
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..914
                     /gene="LOC144113678"
                     /coded_by="XM_077646910.1:215..2959"
                     /db_xref="GeneID:144113678"
ORIGIN      
        1 mtassesnes etgsdtgfet gsdppsvgnr dtprtspspe vvkddvpltg qvldlrhrnl
       61 eslvvrgaft etlerlnvad cglrkapesv wdatslvvfn aasnylqeip pevsrlhrlr
      121 elhvdfnelh elpcelsals qlrvlsasgn rlealpetlg rlpeleslrv aanaiaqlpp
      181 ppalqplqal rrldlranci qgvadlsalq slqaldvswn kieelrlptt nrlrhldcsr
      241 nclrelnlpg grletlcssh nrlqnvditl pcnvlsyidl smnqlaqvpy wvpqarsaqh
      301 ldlshnrlas lpaslfsptl sqldtvrlnh nslasfpesi taqchirhld lqhncltslp
      361 anllrhayrl qwlnasynql sslarpeqts lplrwllltr tgltdelvwp lvlacpglkv
      421 lqlaynqllt vpariselqn lrdlvltgna lqrlpeslas lpaleallln cnqldvlpnf
      481 ehirslkvld vscnllskvt lsslvsptlq qldiscnqal fvdtqefhvl csrrnvsvvd
      541 tkfsgrlapp fksngpgqtc lespfwttgf seslkgparl yinqctqpsg htevlaaile
      601 spqrqllsta kaavaqlvrq errsadssav flqsiilsty ralgskacch qlnivlchla
      661 padasadadg aaylmtvaqc ggatcllaha dkqatilfds ngqttrcvad dwdaavqegf
      721 qthassswps targpppcel pspsirhvtl rpddrlvvls taalgealap daalaealda
      781 pnpmaaakrl nqavaslvsd ggtcviaigl qqaaattggp gksvhfritt diddeqadea
      841 eaykaweyml aqnhkmlfnr eletlhrtla kgksrhaaia dsaivssagt arlgtkkkry
      901 cygqtgealp ssva
//
LOCUS       XP_077503037             685 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113679 [Amblyomma americanum].
ACCESSION   XP_077503037
VERSION     XP_077503037.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646911.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..685
                     /product="uncharacterized protein LOC144113679"
                     /calculated_mol_wt=70763
     Region          <135..291
                     /region_name="LGT"
                     /note="Prolipoprotein diacylglyceryl transferase; cl00478"
                     /db_xref="CDD:469786"
     Region          <489..>677
                     /region_name="PRK07003"
                     /note="DNA polymerase III subunit gamma/tau"
                     /db_xref="CDD:235906"
     CDS             1..685
                     /gene="LOC144113679"
                     /coded_by="XM_077646911.1:160..2217"
                     /db_xref="GeneID:144113679"
ORIGIN      
        1 mgavkviftl alvtttvlsl ptlptplkls ggpgqsaqpv gqsshadsvp ggqadeskst
       61 sepkqaerle gvrsvssdps lttkkhsasd gsvqatqqvs thdvsskdav vqlkksvkln
      121 neeslpltgd aqeggkedtp pivkseesvh esptvphsda sktdtsvpet akpqepakpl
      181 kgiiedvrdl avpedavelp rdvtklsedn ckspvaapgd qaetedqtdl tpeaqrqpsa
      241 qlvpgnpepv aaeseiqehd ektvyegsnk pdeastlasa dlsqaepatp enrpetnhki
      301 skredtkhgf ekdsaahsas dtsssktkta ksevskpaht kmeahkstkp hvktvqetad
      361 etkgfktsre iaspemaevg aresdtvgvv qspdaavpdn sqvspsnqvp ssaapvlsts
      421 asqaavptad aetpqekpss qgandtaknq taiptgggsg fitnplvpir nffqpvfsaa
      481 tavrdqirpv igaalspilg plspgkggea qnastvtaap qvptsegaat satsavtdee
      541 pqkesqtaap tasitaeegs eapevsadgt gaasdaksva lpaleanrnq sqsplgpfpv
      601 afdpvaainq ftkpvvdaat nvrdqvrpif daavapivda lsptkeanna tteagsaepq
      661 lrasdempes aaeepgelnk rrysk
//
LOCUS       XP_077503038            1329 aa            linear   INV 25-NOV-2025
DEFINITION  recQ4 helicase isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503038
VERSION     XP_077503038.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646912.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1329
                     /product="recQ4 helicase isoform X1"
                     /calculated_mol_wt=149766
     Region          5..53
                     /region_name="RecQL4_SLD2_NTD"
                     /note="N-terminal homeodomain-like domain of metazoan RecQ
                     protein-like 4 (RecQL4), fungal DNA replication regulator
                     SLD2 and similar proteins; cd22289"
                     /db_xref="CDD:412085"
     Region          501..516
                     /region_name="zf-CCHC"
                     /note="Zinc knuckle; pfam00098"
                     /db_xref="CDD:395050"
     Region          621..>973
                     /region_name="RecQ"
                     /note="Superfamily II DNA helicase RecQ [Replication,
                     recombination and repair]; COG0514"
                     /db_xref="CDD:440280"
     CDS             1..1329
                     /gene="RecQ4"
                     /coded_by="XM_077646912.1:66..4055"
                     /db_xref="GeneID:144113680"
ORIGIN      
        1 mnlnenvqql kfiikewekt ftaehgrkps addiknapkn iaeaytgywk ikkkvpelka
       61 svalddgvwd sslnkkrppa nenkkrpiss vytskirekl lasfevksqp keafkscgyr
      121 skslqdepvc pkteerrehf tsvsneqqak ectpteqnit aaedsaptsp hkmastsrtl
      181 yykhsldesw lsrnsiaddd evfvsppssa shlpltcrls ayrgrfsekg yvkakvsmkn
      241 sslnssfsel iddvflkpms pktpameqpq tpvpspqlse iaceensscs clvlpeaktr
      301 nennsnhhtq klqhnfphas kqetqvskld gcgdqpsqkv yefekepvge lnslkelaee
      361 phekhnrkrr trvdstnspt qsgdkqqkka vkkpkvsnds gcdeprkrkk kctkpsvtaq
      421 davllderpa vqpkkvgrpn vsrqarsksl nenfvrinlk rkkfsrghhk vnvrqlkfkq
      481 wknkrnqtsw egttstsdas kcfkcgeigh warscpgsts rlevpcepev ahlptldeaa
      541 qmargiksln rsesltklfc sensyeqedk vpavvnesef nlathiknse rtsvqpllka
      601 dhggklpdtp kfvfealykm gfsdfrpgqe ativrilsgl stllvsstgs gkslcyqlpa
      661 ylyaqkmeci tlvvsplisl medqvarlap clkaaflhsa mppaqrqqvl tqiqegrvhf
      721 llvspeslve gssivrelpp vafacideah clsewshnfr paylqlykvl ttqlkvhcil
      781 altatatkst clsiaehlqi dditagiigt psvpenlils vsedqyrdea liellkgtrf
      841 gscesiivyc trreeterla slirtslqhi akevdkedtc kaksrrsyla wdaeayhagm
      901 tpfrrrsvqk kfmtgklrvv vatvafgmgi dkadiravih ynmpksfesy vqetgragrd
      961 gltshchlfl epekkdqnel krhiyansvd rhvirkllkk vlapltkckk sedntstgfn
     1021 mrstyeiaip ieaaveeldl keeniltllc flefhprkvv qllnrvyatc tikcyggpqq
     1081 lrsvasknav vaaamalqkk ineedctnsl sfavvdvaar mgwdsklvks dlkrlewdns
     1141 mlhatghsrk tgvivefsdl afhlnvsasl teedcdelld ylnervqkqe kmdlarlrkv
     1201 heafqsvscq ectecanena stdrsnalkk liqdyfeekp dlqhkdnedt tpteqclgsl
     1261 rqeirslvmq hrdhsfngra varifhgies pcfpakvwgr vhrfwrsfld vdfntilnia
     1321 nkellslrs
//
LOCUS       XP_077503039            1245 aa            linear   INV 25-NOV-2025
DEFINITION  recQ4 helicase isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503039
VERSION     XP_077503039.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646913.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1245
                     /product="recQ4 helicase isoform X2"
                     /calculated_mol_wt=140372
     Region          5..53
                     /region_name="RecQL4_SLD2_NTD"
                     /note="N-terminal homeodomain-like domain of metazoan RecQ
                     protein-like 4 (RecQL4), fungal DNA replication regulator
                     SLD2 and similar proteins; cd22289"
                     /db_xref="CDD:412085"
     Region          501..516
                     /region_name="zf-CCHC"
                     /note="Zinc knuckle; pfam00098"
                     /db_xref="CDD:395050"
     Region          621..>889
                     /region_name="RecQ"
                     /note="Superfamily II DNA helicase RecQ [Replication,
                     recombination and repair]; COG0514"
                     /db_xref="CDD:440280"
     CDS             1..1245
                     /gene="RecQ4"
                     /coded_by="XM_077646913.1:55..3792"
                     /db_xref="GeneID:144113680"
ORIGIN      
        1 mnlnenvqql kfiikewekt ftaehgrkps addiknapkn iaeaytgywk ikkkvpelka
       61 svalddgvwd sslnkkrppa nenkkrpiss vytskirekl lasfevksqp keafkscgyr
      121 skslqdepvc pkteerrehf tsvsneqqak ectpteqnit aaedsaptsp hkmastsrtl
      181 yykhsldesw lsrnsiaddd evfvsppssa shlpltcrls ayrgrfsekg yvkakvsmkn
      241 sslnssfsel iddvflkpms pktpameqpq tpvpspqlse iaceensscs clvlpeaktr
      301 nennsnhhtq klqhnfphas kqetqvskld gcgdqpsqkv yefekepvge lnslkelaee
      361 phekhnrkrr trvdstnspt qsgdkqqkka vkkpkvsnds gcdeprkrkk kctkpsvtaq
      421 davllderpa vqpkkvgrpn vsrqarsksl nenfvrinlk rkkfsrghhk vnvrqlkfkq
      481 wknkrnqtsw egttstsdas kcfkcgeigh warscpgsts rlevpcepev ahlptldeaa
      541 qmargiksln rsesltklfc sensyeqedk vpavvnesef nlathiknse rtsvqpllka
      601 dhggklpdtp kfvfealykm gfsdfrpgqe ativrilsgl stllvsstgs gkslcyqlpa
      661 ylyaqkmeci tlvvsplisl medqvlttql kvhcilalta tatkstclsi aehlqiddit
      721 agiigtpsvp enlilsvsed qyrdealiel lkgtrfgsce siivyctrre eterlaslir
      781 tslqhiakev dkedtckaks rrsylawdae ayhagmtpfr rrsvqkkfmt gklrvvvatv
      841 afgmgidkad iravihynmp ksfesyvqet gragrdglts hchlflepek kdqnelkrhi
      901 yansvdrhvi rkllkkvlap ltkckksedn tstgfnmrst yeiaipieaa veeldlkeen
      961 iltllcflef hprkvvqlln rvyatctikc yggpqqlrsv asknavvaaa malqkkinee
     1021 dctnslsfav vdvaarmgwd sklvksdlkr lewdnsmlha tghsrktgvi vefsdlafhl
     1081 nvsaslteed cdelldylne rvqkqekmdl arlrkvheaf qsvscqecte canenastdr
     1141 snalkkliqd yfeekpdlqh kdnedttpte qclgslrqei rslvmqhrdh sfngravari
     1201 fhgiespcfp akvwgrvhrf wrsfldvdfn tilniankel lslrs
//
LOCUS       XP_077503040             316 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier family 25 member 44 [Amblyomma americanum].
ACCESSION   XP_077503040
VERSION     XP_077503040.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646914.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..316
                     /product="solute carrier family 25 member 44"
                     /calculated_mol_wt=36563
     Region          20..103
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          111..219
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          224..304
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     CDS             1..316
                     /gene="LOC144113682"
                     /coded_by="XM_077646914.1:321..1271"
                     /db_xref="GeneID:144113682"
ORIGIN      
        1 medpeiltie wemmdksrff mfsminsftl rclvypltvi ktrlqvqkpg klytgtfdac
       61 skilryegfg glyrgfwint iqmfsgigyi ftyekvrdml srhadihdrr lkgligggcs
      121 slvsqtiitp fdvvsqhmmv lgrssksggv vmnplnisvd qkkkhlifaa ivrelyrrdg
      181 fsgfyrgyfa sllayvpgsa lwwmfypvys dglrrvlpgw tpqmfvqcma gpmsgvtvcf
      241 itnpmdvvra riqvqrmnsv mqtfwqlwte ermrmfqigl sarvmqslis sfllalgyet
      301 lkrwsvheey kdkirw
//
LOCUS       XP_077503041             538 aa            linear   INV 25-NOV-2025
DEFINITION  putative aminopeptidase NPEPL1 granny smith protein isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077503041
VERSION     XP_077503041.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646915.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..538
                     /product="putative aminopeptidase NPEPL1 granny smith
                     protein isoform X1"
                     /calculated_mol_wt=57372
     Region          39..505
                     /region_name="Peptidase_M17"
                     /note="Cytosol aminopeptidase family, N-terminal and
                     catalytic domains. Family M17 contains zinc- and
                     manganese-dependent exopeptidases (EC 3.4.11.1), including
                     leucine aminopeptidase. They catalyze removal of amino
                     acids from the N-terminus of a protein and...; cd00433"
                     /db_xref="CDD:238247"
     Site            order(73..74,89,97,105,204,206..208,252,254,282,288,293,
                     296,299..300,332,334..337,339,341..345,351,353,357,
                     394..398,401,427..428,430,436,438..439,444,446,457..458,
                     460,491)
                     /site_type="other"
                     /note="interface (dimer of trimers) [polypeptide binding]"
                     /db_xref="CDD:238247"
     Site            order(278,283,290,301,360,362,364,389)
                     /site_type="active"
                     /note="Substrate-binding/catalytic site [active]"
                     /db_xref="CDD:238247"
     Site            order(278,283,301,360,362)
                     /site_type="other"
                     /note="Zn-binding sites [ion binding]"
                     /db_xref="CDD:238247"
     CDS             1..538
                     /gene="grsm"
                     /coded_by="XM_077646915.1:147..1763"
                     /db_xref="GeneID:144113683"
ORIGIN      
        1 mehkskrasg ngcrlrnlam cctvikfngh ltpsdplhhp vliigqpknl akitfedvkc
       61 kleprvgeem fkaavlslhp sptdncflyl nlaivaalpt kcsrhntpsh ahsltklvkn
      121 ltagvdeyiv vvcewqdvfa sacavarafp vysrkssgnp gshsvtvefl lvdgggggrh
      181 lgepelrcls aaaegvqlta kivdtpchdm nteafikeve ivgnalglkp tiikgeelni
      241 rgfgglysvg raavhppalv ilshtpegsq dtiawvgkgi vydtgglcik sktamcgmkr
      301 dcggaagilg afyatvklgf sdnlhaifcl aenavgpdat rpddiitmys grtveinntd
      361 aegrlvvgdg vayahkdlka niildmatlt gaqgiatgky haalvsnssd weelamrsgi
      421 lsgdlihamp fapelhfvef asaladmkns vadrnnaqps caglficshl gfefpgvwmh
      481 vdmaypvhcg eratgygval lltlfghcsk npllkelssw ctanaddver atkkirlv
//
LOCUS       XP_077503042             519 aa            linear   INV 25-NOV-2025
DEFINITION  putative aminopeptidase NPEPL1 granny smith protein isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077503042
VERSION     XP_077503042.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646916.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..519
                     /product="putative aminopeptidase NPEPL1 granny smith
                     protein isoform X2"
                     /calculated_mol_wt=55262
     Region          20..486
                     /region_name="Peptidase_M17"
                     /note="Cytosol aminopeptidase family, N-terminal and
                     catalytic domains. Family M17 contains zinc- and
                     manganese-dependent exopeptidases (EC 3.4.11.1), including
                     leucine aminopeptidase. They catalyze removal of amino
                     acids from the N-terminus of a protein and...; cd00433"
                     /db_xref="CDD:238247"
     Site            order(54..55,70,78,86,185,187..189,233,235,263,269,274,
                     277,280..281,313,315..318,320,322..326,332,334,338,
                     375..379,382,408..409,411,417,419..420,425,427,438..439,
                     441,472)
                     /site_type="other"
                     /note="interface (dimer of trimers) [polypeptide binding]"
                     /db_xref="CDD:238247"
     Site            order(259,264,271,282,341,343,345,370)
                     /site_type="active"
                     /note="Substrate-binding/catalytic site [active]"
                     /db_xref="CDD:238247"
     Site            order(259,264,282,341,343)
                     /site_type="other"
                     /note="Zn-binding sites [ion binding]"
                     /db_xref="CDD:238247"
     CDS             1..519
                     /gene="grsm"
                     /coded_by="XM_077646916.1:185..1744"
                     /db_xref="GeneID:144113683"
ORIGIN      
        1 mcctvikfng hltpsdplhh pvliigqpkn lakitfedvk ckleprvgee mfkaavlslh
       61 psptdncfly lnlaivaalp tkcsrhntps hahsltklvk nltagvdeyi vvvcewqdvf
      121 asacavaraf pvysrkssgn pgshsvtvef llvdgggggr hlgepelrcl saaaegvqlt
      181 akivdtpchd mnteafikev eivgnalglk ptiikgeeln irgfgglysv graavhppal
      241 vilshtpegs qdtiawvgkg ivydtgglci ksktamcgmk rdcggaagil gafyatvklg
      301 fsdnlhaifc laenavgpda trpddiitmy sgrtveinnt daegrlvvgd gvayahkdlk
      361 aniildmatl tgaqgiatgk yhaalvsnss dweelamrsg ilsgdliham pfapelhfve
      421 fasaladmkn svadrnnaqp scaglficsh lgfefpgvwm hvdmaypvhc geratgygva
      481 llltlfghcs knpllkelss wctanaddve ratkkirlv
//
LOCUS       XP_077503043             519 aa            linear   INV 25-NOV-2025
DEFINITION  putative aminopeptidase NPEPL1 granny smith protein isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077503043
VERSION     XP_077503043.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646917.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..519
                     /product="putative aminopeptidase NPEPL1 granny smith
                     protein isoform X2"
                     /calculated_mol_wt=55262
     Region          20..486
                     /region_name="Peptidase_M17"
                     /note="Cytosol aminopeptidase family, N-terminal and
                     catalytic domains. Family M17 contains zinc- and
                     manganese-dependent exopeptidases (EC 3.4.11.1), including
                     leucine aminopeptidase. They catalyze removal of amino
                     acids from the N-terminus of a protein and...; cd00433"
                     /db_xref="CDD:238247"
     Site            order(54..55,70,78,86,185,187..189,233,235,263,269,274,
                     277,280..281,313,315..318,320,322..326,332,334,338,
                     375..379,382,408..409,411,417,419..420,425,427,438..439,
                     441,472)
                     /site_type="other"
                     /note="interface (dimer of trimers) [polypeptide binding]"
                     /db_xref="CDD:238247"
     Site            order(259,264,271,282,341,343,345,370)
                     /site_type="active"
                     /note="Substrate-binding/catalytic site [active]"
                     /db_xref="CDD:238247"
     Site            order(259,264,282,341,343)
                     /site_type="other"
                     /note="Zn-binding sites [ion binding]"
                     /db_xref="CDD:238247"
     CDS             1..519
                     /gene="grsm"
                     /coded_by="XM_077646917.1:174..1733"
                     /db_xref="GeneID:144113683"
ORIGIN      
        1 mcctvikfng hltpsdplhh pvliigqpkn lakitfedvk ckleprvgee mfkaavlslh
       61 psptdncfly lnlaivaalp tkcsrhntps hahsltklvk nltagvdeyi vvvcewqdvf
      121 asacavaraf pvysrkssgn pgshsvtvef llvdgggggr hlgepelrcl saaaegvqlt
      181 akivdtpchd mnteafikev eivgnalglk ptiikgeeln irgfgglysv graavhppal
      241 vilshtpegs qdtiawvgkg ivydtgglci ksktamcgmk rdcggaagil gafyatvklg
      301 fsdnlhaifc laenavgpda trpddiitmy sgrtveinnt daegrlvvgd gvayahkdlk
      361 aniildmatl tgaqgiatgk yhaalvsnss dweelamrsg ilsgdliham pfapelhfve
      421 fasaladmkn svadrnnaqp scaglficsh lgfefpgvwm hvdmaypvhc geratgygva
      481 llltlfghcs knpllkelss wctanaddve ratkkirlv
//
LOCUS       XP_077503044             519 aa            linear   INV 25-NOV-2025
DEFINITION  putative aminopeptidase NPEPL1 granny smith protein isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077503044
VERSION     XP_077503044.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646918.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..519
                     /product="putative aminopeptidase NPEPL1 granny smith
                     protein isoform X2"
                     /calculated_mol_wt=55262
     Region          20..486
                     /region_name="Peptidase_M17"
                     /note="Cytosol aminopeptidase family, N-terminal and
                     catalytic domains. Family M17 contains zinc- and
                     manganese-dependent exopeptidases (EC 3.4.11.1), including
                     leucine aminopeptidase. They catalyze removal of amino
                     acids from the N-terminus of a protein and...; cd00433"
                     /db_xref="CDD:238247"
     Site            order(54..55,70,78,86,185,187..189,233,235,263,269,274,
                     277,280..281,313,315..318,320,322..326,332,334,338,
                     375..379,382,408..409,411,417,419..420,425,427,438..439,
                     441,472)
                     /site_type="other"
                     /note="interface (dimer of trimers) [polypeptide binding]"
                     /db_xref="CDD:238247"
     Site            order(259,264,271,282,341,343,345,370)
                     /site_type="active"
                     /note="Substrate-binding/catalytic site [active]"
                     /db_xref="CDD:238247"
     Site            order(259,264,282,341,343)
                     /site_type="other"
                     /note="Zn-binding sites [ion binding]"
                     /db_xref="CDD:238247"
     CDS             1..519
                     /gene="grsm"
                     /coded_by="XM_077646918.1:134..1693"
                     /db_xref="GeneID:144113683"
ORIGIN      
        1 mcctvikfng hltpsdplhh pvliigqpkn lakitfedvk ckleprvgee mfkaavlslh
       61 psptdncfly lnlaivaalp tkcsrhntps hahsltklvk nltagvdeyi vvvcewqdvf
      121 asacavaraf pvysrkssgn pgshsvtvef llvdgggggr hlgepelrcl saaaegvqlt
      181 akivdtpchd mnteafikev eivgnalglk ptiikgeeln irgfgglysv graavhppal
      241 vilshtpegs qdtiawvgkg ivydtgglci ksktamcgmk rdcggaagil gafyatvklg
      301 fsdnlhaifc laenavgpda trpddiitmy sgrtveinnt daegrlvvgd gvayahkdlk
      361 aniildmatl tgaqgiatgk yhaalvsnss dweelamrsg ilsgdliham pfapelhfve
      421 fasaladmkn svadrnnaqp scaglficsh lgfefpgvwm hvdmaypvhc geratgygva
      481 llltlfghcs knpllkelss wctanaddve ratkkirlv
//
LOCUS       XP_077503045             480 aa            linear   INV 25-NOV-2025
DEFINITION  octopamine receptor in mushroom bodies isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503045
VERSION     XP_077503045.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646919.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..480
                     /product="octopamine receptor in mushroom bodies isoform
                     X1"
                     /calculated_mol_wt=52724
     Region          20..442
                     /region_name="7tmA_Octopamine_R"
                     /note="octopamine receptors in invertebrates, member of
                     the class A family of seven-transmembrane G
                     protein-coupled receptors; cd15063"
                     /db_xref="CDD:320191"
     Region          21..47
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320191"
     Region          54..80
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320191"
     Region          92..122
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320191"
     Site            order(95,99..100,103..104,196,203,206..207,210,391,
                     394..395,398,413,417,421)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:320191"
     Region          134..157
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320191"
     Region          199..228
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320191"
     Region          371..401
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320191"
     Region          410..435
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320191"
     CDS             1..480
                     /gene="Oamb"
                     /coded_by="XM_077646919.1:425..1867"
                     /db_xref="GeneID:144113684"
ORIGIN      
        1 mnatclssvp peklhepgtv atflvlglin vlvifgnllv mvavlastkl rtvtnyfvvs
       61 lavadlsvgi ivlpysivle vlevwifght wcqiwlavdv wlctssilnl caisvdryla
      121 itrpvryrsl mssrraklli vavwvtafvi cfpplvgwnd gtqnsvpyrg lnetslnass
      181 vadaplplce saqcvlinnk gyviysalgs fyipmlfmlf fnyriyraai qtgralergf
      241 ittksgkikg rtqdqrltlr vhrgndsamn akrgsehlga etcidgivtg rrrpglkksr
      301 depsasarss askarqqsdq ratrsappsf ksnrgsvrns grngtssggg gggkgsrssk
      361 rsqrwqakrf rteakatktv gtivggficc wlpfftvylv rafcehcipn llfsvffwlg
      421 ycnsainpli yalvskdfrl afkrilcrcr lkeggvssli kqihmltvld dappdnaesp
//
LOCUS       XP_077503046             480 aa            linear   INV 25-NOV-2025
DEFINITION  octopamine receptor in mushroom bodies isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503046
VERSION     XP_077503046.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646920.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..480
                     /product="octopamine receptor in mushroom bodies isoform
                     X1"
                     /calculated_mol_wt=52724
     Region          20..442
                     /region_name="7tmA_Octopamine_R"
                     /note="octopamine receptors in invertebrates, member of
                     the class A family of seven-transmembrane G
                     protein-coupled receptors; cd15063"
                     /db_xref="CDD:320191"
     Region          21..47
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320191"
     Region          54..80
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320191"
     Region          92..122
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320191"
     Site            order(95,99..100,103..104,196,203,206..207,210,391,
                     394..395,398,413,417,421)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:320191"
     Region          134..157
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320191"
     Region          199..228
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320191"
     Region          371..401
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320191"
     Region          410..435
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320191"
     CDS             1..480
                     /gene="Oamb"
                     /coded_by="XM_077646920.1:596..2038"
                     /db_xref="GeneID:144113684"
ORIGIN      
        1 mnatclssvp peklhepgtv atflvlglin vlvifgnllv mvavlastkl rtvtnyfvvs
       61 lavadlsvgi ivlpysivle vlevwifght wcqiwlavdv wlctssilnl caisvdryla
      121 itrpvryrsl mssrraklli vavwvtafvi cfpplvgwnd gtqnsvpyrg lnetslnass
      181 vadaplplce saqcvlinnk gyviysalgs fyipmlfmlf fnyriyraai qtgralergf
      241 ittksgkikg rtqdqrltlr vhrgndsamn akrgsehlga etcidgivtg rrrpglkksr
      301 depsasarss askarqqsdq ratrsappsf ksnrgsvrns grngtssggg gggkgsrssk
      361 rsqrwqakrf rteakatktv gtivggficc wlpfftvylv rafcehcipn llfsvffwlg
      421 ycnsainpli yalvskdfrl afkrilcrcr lkeggvssli kqihmltvld dappdnaesp
//
LOCUS       XP_077503047             480 aa            linear   INV 25-NOV-2025
DEFINITION  octopamine receptor in mushroom bodies isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503047
VERSION     XP_077503047.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646921.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..480
                     /product="octopamine receptor in mushroom bodies isoform
                     X1"
                     /calculated_mol_wt=52724
     Region          20..442
                     /region_name="7tmA_Octopamine_R"
                     /note="octopamine receptors in invertebrates, member of
                     the class A family of seven-transmembrane G
                     protein-coupled receptors; cd15063"
                     /db_xref="CDD:320191"
     Region          21..47
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320191"
     Region          54..80
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320191"
     Region          92..122
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320191"
     Site            order(95,99..100,103..104,196,203,206..207,210,391,
                     394..395,398,413,417,421)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:320191"
     Region          134..157
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320191"
     Region          199..228
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320191"
     Region          371..401
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320191"
     Region          410..435
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320191"
     CDS             1..480
                     /gene="Oamb"
                     /coded_by="XM_077646921.1:351..1793"
                     /db_xref="GeneID:144113684"
ORIGIN      
        1 mnatclssvp peklhepgtv atflvlglin vlvifgnllv mvavlastkl rtvtnyfvvs
       61 lavadlsvgi ivlpysivle vlevwifght wcqiwlavdv wlctssilnl caisvdryla
      121 itrpvryrsl mssrraklli vavwvtafvi cfpplvgwnd gtqnsvpyrg lnetslnass
      181 vadaplplce saqcvlinnk gyviysalgs fyipmlfmlf fnyriyraai qtgralergf
      241 ittksgkikg rtqdqrltlr vhrgndsamn akrgsehlga etcidgivtg rrrpglkksr
      301 depsasarss askarqqsdq ratrsappsf ksnrgsvrns grngtssggg gggkgsrssk
      361 rsqrwqakrf rteakatktv gtivggficc wlpfftvylv rafcehcipn llfsvffwlg
      421 ycnsainpli yalvskdfrl afkrilcrcr lkeggvssli kqihmltvld dappdnaesp
//
LOCUS       XP_077503048             464 aa            linear   INV 25-NOV-2025
DEFINITION  octopamine receptor in mushroom bodies isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503048
VERSION     XP_077503048.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646922.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..464
                     /product="octopamine receptor in mushroom bodies isoform
                     X2"
                     /calculated_mol_wt=51101
     CDS             1..464
                     /gene="Oamb"
                     /coded_by="XM_077646922.1:131..1525"
                     /db_xref="GeneID:144113684"
ORIGIN      
        1 mniraltfls taasmaaksq nlakdptksg vqtsggptms sdahpvqahd vnqrqvlavk
       61 dpkgqfyggg tptqsqstgk skegqpptvl svaacscelk dqqhvhvalt spcvmprttd
      121 astqtytgsl ckentqdhag grsgrqelsk lkepsvyqkr rnnsgsnenp lktsaatlqf
      181 rsgiptpskk kplikgteqe rgshveapdd grsgalfkqn mmerlakand ilremlaepp
      241 sstsiisaap ktvqpcpvkn sefstavsrs tpiretsvrp knadrkeipk tqkqcpsest
      301 slfidagqyi atdelwsink tiksqwkkar akihedaset ynkflldsce hmskltgatr
      361 eserlkvvka rkellasmkk evhiacgafe ewrthylqde lqkcctaler gghsfvlaav
      421 lyeeafkalr dkearthshv stlwmelikv ekmnqdffsy fini
//
LOCUS       XP_077503049             452 aa            linear   INV 25-NOV-2025
DEFINITION  dopamine receptor 2-like [Amblyomma americanum].
ACCESSION   XP_077503049
VERSION     XP_077503049.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646923.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..452
                     /product="dopamine receptor 2-like"
                     /calculated_mol_wt=50123
     Region          73..410
                     /region_name="7tmA_Dop1R2-like"
                     /note="dopamine 1-like receptor 2 from Drosophila
                     melanogaster and similar proteins, member of the class A
                     family of seven-transmembrane G protein-coupled receptors;
                     cd15067"
                     /db_xref="CDD:320195"
     Region          73..99
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320195"
     Region          106..132
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320195"
     Region          145..175
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320195"
     Site            order(148,152..153,156..157,226,233,236..237,240,356,
                     359..360,363,381,385,389)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:320195"
     Region          187..209
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320195"
     Region          229..258
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320195"
     Region          336..366
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320195"
     Region          378..403
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320195"
     CDS             1..452
                     /gene="LOC144113686"
                     /coded_by="XM_077646923.1:214..1572"
                     /db_xref="GeneID:144113686"
ORIGIN      
        1 mpalgeangs wdspaysvle llygsnassv elevlgngtv ldivrdanls lleesnanat
       61 aateesvhvq haalgvllgf fcfatvfgnv limvsvakeq ylhtvtnyfi aslatadclv
      121 gavvmpfsai heimnkywif gqdlcdvwhs idvlastasi lnlcvisldr ywaitdpisy
      181 pcrmtqarat tliavvwvcs alisfpaiaw wravtklppa afqcaftddv gylvfsstis
      241 fyaplmvmvf tyyriykaaa eqtrnlklgc kqvqscngee stsvtlrihr ggmlqtanny
      301 kavfstsnen fdrnnvtgts rnvknfslsr klaklakerk aaktlaivmg vfilcwlpff
      361 vtnilmgicg eacvmqpdlv fstvtwlgwi nsgmnpviya cwsrdfrraf anvlccccpg
      421 yfrkrqrrrd rlrrmikeda slrshsieea vl
//
LOCUS       XP_077503050             452 aa            linear   INV 25-NOV-2025
DEFINITION  dopamine receptor 2-like [Amblyomma americanum].
ACCESSION   XP_077503050
VERSION     XP_077503050.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646924.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..452
                     /product="dopamine receptor 2-like"
                     /calculated_mol_wt=50123
     Region          73..410
                     /region_name="7tmA_Dop1R2-like"
                     /note="dopamine 1-like receptor 2 from Drosophila
                     melanogaster and similar proteins, member of the class A
                     family of seven-transmembrane G protein-coupled receptors;
                     cd15067"
                     /db_xref="CDD:320195"
     Region          73..99
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320195"
     Region          106..132
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320195"
     Region          145..175
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320195"
     Site            order(148,152..153,156..157,226,233,236..237,240,356,
                     359..360,363,381,385,389)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:320195"
     Region          187..209
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320195"
     Region          229..258
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320195"
     Region          336..366
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320195"
     Region          378..403
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320195"
     CDS             1..452
                     /gene="LOC144113686"
                     /coded_by="XM_077646924.1:527..1885"
                     /db_xref="GeneID:144113686"
ORIGIN      
        1 mpalgeangs wdspaysvle llygsnassv elevlgngtv ldivrdanls lleesnanat
       61 aateesvhvq haalgvllgf fcfatvfgnv limvsvakeq ylhtvtnyfi aslatadclv
      121 gavvmpfsai heimnkywif gqdlcdvwhs idvlastasi lnlcvisldr ywaitdpisy
      181 pcrmtqarat tliavvwvcs alisfpaiaw wravtklppa afqcaftddv gylvfsstis
      241 fyaplmvmvf tyyriykaaa eqtrnlklgc kqvqscngee stsvtlrihr ggmlqtanny
      301 kavfstsnen fdrnnvtgts rnvknfslsr klaklakerk aaktlaivmg vfilcwlpff
      361 vtnilmgicg eacvmqpdlv fstvtwlgwi nsgmnpviya cwsrdfrraf anvlccccpg
      421 yfrkrqrrrd rlrrmikeda slrshsieea vl
//
LOCUS       XP_077503052             534 aa            linear   INV 25-NOV-2025
DEFINITION  hepatocyte nuclear factor 4 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503052
VERSION     XP_077503052.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646926.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..534
                     /product="hepatocyte nuclear factor 4 isoform X1"
                     /calculated_mol_wt=59183
     Region          101..176
                     /region_name="NR_DBD_HNF4A"
                     /note="DNA-binding domain of heptocyte nuclear factor 4
                     (HNF4) is composed of two C4-type zinc fingers; cd06960"
                     /db_xref="CDD:143518"
     Site            order(101,104,118,121,137,143,153,156)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143518"
     Site            order(107,111..113,119..120,122,124,126..127,130,150..151,
                     154,157,170..172,174)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143518"
     Site            order(138,151..152,154)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:143518"
     Region          195..430
                     /region_name="NR_LBD_HNF4_like"
                     /note="The ligand binding domain of heptocyte nuclear
                     factor 4, which is explosively expanded in nematodes;
                     cd06931"
                     /db_xref="CDD:132729"
     Site            order(226,232,236,270,274,277,287..288,305,411..412,415)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132729"
     Site            order(241,245,255..256,258..259,262..263,425..426,
                     429..430)
                     /site_type="other"
                     /note="coactivator recognition site [polypeptide binding]"
                     /db_xref="CDD:132729"
     Site            order(312,333,349,369..370,373..374,377..378,388..389,
                     391..393,395..396,398..400,402..403,406)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:132729"
     CDS             1..534
                     /gene="Hnf4"
                     /coded_by="XM_077646926.1:157..1761"
                     /db_xref="GeneID:144113687"
ORIGIN      
        1 mnagryenfa yegpplyfgg qqpdyappqp pvaqpvvldr aslrgadeqq ssdcscgssp
       61 drwarvrppp pppppppreh dselayssqq sgtpsvlsqf caicgdratg khygaascdg
      121 ckgffrrsvr knhlyscrfn rscvvdkdkr nqcrycrlkk cfragmkkea vqnerdrisn
      181 rrpsyeesnn ssglsvtnll naemysrnis spvseynisn kkianisdvc esmkqqllil
      241 vdwaksvpsf telsiedqva llrahagehl llglarrsmh tknvlllgnd fiiprvstev
      301 eisrigarim delvttlrev hiddtefacl raivffdpre ltppsvhlda cslthtkgls
      361 epqkikalrh qvqtnledyi ndrqyesrgr fgeimlllpt lqsitwqmie qiqfaklfgm
      421 akvdnllqem llggmvgpqa fynqgsspdc psgastspls lsssvihqsi lqsnagienl
      481 tngrrcdgne lgassrpqpd tpipsppdpt gfkpmqdlgv qafktevlet daty
//
LOCUS       XP_077503053             534 aa            linear   INV 25-NOV-2025
DEFINITION  hepatocyte nuclear factor 4 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503053
VERSION     XP_077503053.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646927.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..534
                     /product="hepatocyte nuclear factor 4 isoform X1"
                     /calculated_mol_wt=59183
     Region          101..176
                     /region_name="NR_DBD_HNF4A"
                     /note="DNA-binding domain of heptocyte nuclear factor 4
                     (HNF4) is composed of two C4-type zinc fingers; cd06960"
                     /db_xref="CDD:143518"
     Site            order(101,104,118,121,137,143,153,156)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143518"
     Site            order(107,111..113,119..120,122,124,126..127,130,150..151,
                     154,157,170..172,174)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143518"
     Site            order(138,151..152,154)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:143518"
     Region          195..430
                     /region_name="NR_LBD_HNF4_like"
                     /note="The ligand binding domain of heptocyte nuclear
                     factor 4, which is explosively expanded in nematodes;
                     cd06931"
                     /db_xref="CDD:132729"
     Site            order(226,232,236,270,274,277,287..288,305,411..412,415)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132729"
     Site            order(241,245,255..256,258..259,262..263,425..426,
                     429..430)
                     /site_type="other"
                     /note="coactivator recognition site [polypeptide binding]"
                     /db_xref="CDD:132729"
     Site            order(312,333,349,369..370,373..374,377..378,388..389,
                     391..393,395..396,398..400,402..403,406)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:132729"
     CDS             1..534
                     /gene="Hnf4"
                     /coded_by="XM_077646927.1:146..1750"
                     /db_xref="GeneID:144113687"
ORIGIN      
        1 mnagryenfa yegpplyfgg qqpdyappqp pvaqpvvldr aslrgadeqq ssdcscgssp
       61 drwarvrppp pppppppreh dselayssqq sgtpsvlsqf caicgdratg khygaascdg
      121 ckgffrrsvr knhlyscrfn rscvvdkdkr nqcrycrlkk cfragmkkea vqnerdrisn
      181 rrpsyeesnn ssglsvtnll naemysrnis spvseynisn kkianisdvc esmkqqllil
      241 vdwaksvpsf telsiedqva llrahagehl llglarrsmh tknvlllgnd fiiprvstev
      301 eisrigarim delvttlrev hiddtefacl raivffdpre ltppsvhlda cslthtkgls
      361 epqkikalrh qvqtnledyi ndrqyesrgr fgeimlllpt lqsitwqmie qiqfaklfgm
      421 akvdnllqem llggmvgpqa fynqgsspdc psgastspls lsssvihqsi lqsnagienl
      481 tngrrcdgne lgassrpqpd tpipsppdpt gfkpmqdlgv qafktevlet daty
//
LOCUS       XP_077503054             518 aa            linear   INV 25-NOV-2025
DEFINITION  hepatocyte nuclear factor 4 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503054
VERSION     XP_077503054.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646928.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..518
                     /product="hepatocyte nuclear factor 4 isoform X2"
                     /calculated_mol_wt=57462
     Region          101..176
                     /region_name="NR_DBD_HNF4A"
                     /note="DNA-binding domain of heptocyte nuclear factor 4
                     (HNF4) is composed of two C4-type zinc fingers; cd06960"
                     /db_xref="CDD:143518"
     Site            order(101,104,118,121,137,143,153,156)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143518"
     Site            order(107,111..113,119..120,122,124,126..127,130,150..151,
                     154,157,170..172,174)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143518"
     Site            order(138,151..152,154)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:143518"
     Region          195..414
                     /region_name="NR_LBD_HNF4_like"
                     /note="The ligand binding domain of heptocyte nuclear
                     factor 4, which is explosively expanded in nematodes;
                     cd06931"
                     /db_xref="CDD:132729"
     Site            order(226,232,236,270,274,277,287..288,305,395..396,399)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132729"
     Site            order(241,245,255..256,258..259,262..263,409..410,
                     413..414)
                     /site_type="other"
                     /note="coactivator recognition site [polypeptide binding]"
                     /db_xref="CDD:132729"
     Site            order(312,333,339,353..354,357..358,361..362,372..373,
                     375..377,379..380,382..384,386..387,390)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:132729"
     CDS             1..518
                     /gene="Hnf4"
                     /coded_by="XM_077646928.1:157..1713"
                     /db_xref="GeneID:144113687"
ORIGIN      
        1 mnagryenfa yegpplyfgg qqpdyappqp pvaqpvvldr aslrgadeqq ssdcscgssp
       61 drwarvrppp pppppppreh dselayssqq sgtpsvlsqf caicgdratg khygaascdg
      121 ckgffrrsvr knhlyscrfn rscvvdkdkr nqcrycrlkk cfragmkkea vqnerdrisn
      181 rrpsyeesnn ssglsvtnll naemysrnis spvseynisn kkianisdvc esmkqqllil
      241 vdwaksvpsf telsiedqva llrahagehl llglarrsmh tknvlllgnd fiiprvstev
      301 eisrigarim delvttlrev hiddtefacl raivffdpht kglsepqkik alrhqvqtnl
      361 edyindrqye srgrfgeiml llptlqsitw qmieqiqfak lfgmakvdnl lqemllggmv
      421 gpqafynqgs spdcpsgast splslsssvi hqsilqsnag ienltngrrc dgnelgassr
      481 pqpdtpipsp pdptgfkpmq dlgvqafkte vletdaty
//
LOCUS       XP_077503055             518 aa            linear   INV 25-NOV-2025
DEFINITION  hepatocyte nuclear factor 4 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503055
VERSION     XP_077503055.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646929.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..518
                     /product="hepatocyte nuclear factor 4 isoform X2"
                     /calculated_mol_wt=57462
     Region          101..176
                     /region_name="NR_DBD_HNF4A"
                     /note="DNA-binding domain of heptocyte nuclear factor 4
                     (HNF4) is composed of two C4-type zinc fingers; cd06960"
                     /db_xref="CDD:143518"
     Site            order(101,104,118,121,137,143,153,156)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143518"
     Site            order(107,111..113,119..120,122,124,126..127,130,150..151,
                     154,157,170..172,174)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143518"
     Site            order(138,151..152,154)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:143518"
     Region          195..414
                     /region_name="NR_LBD_HNF4_like"
                     /note="The ligand binding domain of heptocyte nuclear
                     factor 4, which is explosively expanded in nematodes;
                     cd06931"
                     /db_xref="CDD:132729"
     Site            order(226,232,236,270,274,277,287..288,305,395..396,399)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132729"
     Site            order(241,245,255..256,258..259,262..263,409..410,
                     413..414)
                     /site_type="other"
                     /note="coactivator recognition site [polypeptide binding]"
                     /db_xref="CDD:132729"
     Site            order(312,333,339,353..354,357..358,361..362,372..373,
                     375..377,379..380,382..384,386..387,390)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:132729"
     CDS             1..518
                     /gene="Hnf4"
                     /coded_by="XM_077646929.1:146..1702"
                     /db_xref="GeneID:144113687"
ORIGIN      
        1 mnagryenfa yegpplyfgg qqpdyappqp pvaqpvvldr aslrgadeqq ssdcscgssp
       61 drwarvrppp pppppppreh dselayssqq sgtpsvlsqf caicgdratg khygaascdg
      121 ckgffrrsvr knhlyscrfn rscvvdkdkr nqcrycrlkk cfragmkkea vqnerdrisn
      181 rrpsyeesnn ssglsvtnll naemysrnis spvseynisn kkianisdvc esmkqqllil
      241 vdwaksvpsf telsiedqva llrahagehl llglarrsmh tknvlllgnd fiiprvstev
      301 eisrigarim delvttlrev hiddtefacl raivffdpht kglsepqkik alrhqvqtnl
      361 edyindrqye srgrfgeiml llptlqsitw qmieqiqfak lfgmakvdnl lqemllggmv
      421 gpqafynqgs spdcpsgast splslsssvi hqsilqsnag ienltngrrc dgnelgassr
      481 pqpdtpipsp pdptgfkpmq dlgvqafkte vletdaty
//
LOCUS       XP_077503056             492 aa            linear   INV 25-NOV-2025
DEFINITION  hepatocyte nuclear factor 4 isoform X3 [Amblyomma americanum].
ACCESSION   XP_077503056
VERSION     XP_077503056.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646930.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..492
                     /product="hepatocyte nuclear factor 4 isoform X3"
                     /calculated_mol_wt=53898
     Region          59..134
                     /region_name="NR_DBD_HNF4A"
                     /note="DNA-binding domain of heptocyte nuclear factor 4
                     (HNF4) is composed of two C4-type zinc fingers; cd06960"
                     /db_xref="CDD:143518"
     Site            order(59,62,76,79,95,101,111,114)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143518"
     Site            order(65,69..71,77..78,80,82,84..85,88,108..109,112,115,
                     128..130,132)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143518"
     Site            order(96,109..110,112)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:143518"
     Region          153..388
                     /region_name="NR_LBD_HNF4_like"
                     /note="The ligand binding domain of heptocyte nuclear
                     factor 4, which is explosively expanded in nematodes;
                     cd06931"
                     /db_xref="CDD:132729"
     Site            order(184,190,194,228,232,235,245..246,263,369..370,373)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132729"
     Site            order(199,203,213..214,216..217,220..221,383..384,
                     387..388)
                     /site_type="other"
                     /note="coactivator recognition site [polypeptide binding]"
                     /db_xref="CDD:132729"
     Site            order(270,291,307,327..328,331..332,335..336,346..347,
                     349..351,353..354,356..358,360..361,364)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:132729"
     CDS             1..492
                     /gene="Hnf4"
                     /coded_by="XM_077646930.1:114..1592"
                     /db_xref="GeneID:144113687"
ORIGIN      
        1 mnaepsgasa gllgcaaplg svivqggppa maidasgqds elayssqqsg tpsvlsqfca
       61 icgdratgkh ygaascdgck gffrrsvrkn hlyscrfnrs cvvdkdkrnq crycrlkkcf
      121 ragmkkeavq nerdrisnrr psyeesnnss glsvtnllna emysrnissp vseynisnkk
      181 ianisdvces mkqqllilvd waksvpsfte lsiedqvall rahagehlll glarrsmhtk
      241 nvlllgndfi iprvstevei srigarimde lvttlrevhi ddtefaclra ivffdprelt
      301 ppsvhldacs lthtkglsep qkikalrhqv qtnledyind rqyesrgrfg eimlllptlq
      361 sitwqmieqi qfaklfgmak vdnllqemll ggmvgpqafy nqgsspdcps gastsplsls
      421 ssvihqsilq snagienltn grrcdgnelg assrpqpdtp ipsppdptgf kpmqdlgvqa
      481 fktevletda ty
//
LOCUS       XP_077503057             476 aa            linear   INV 25-NOV-2025
DEFINITION  hepatocyte nuclear factor 4 isoform X4 [Amblyomma americanum].
ACCESSION   XP_077503057
VERSION     XP_077503057.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646931.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..476
                     /product="hepatocyte nuclear factor 4 isoform X4"
                     /calculated_mol_wt=52177
     Region          59..134
                     /region_name="NR_DBD_HNF4A"
                     /note="DNA-binding domain of heptocyte nuclear factor 4
                     (HNF4) is composed of two C4-type zinc fingers; cd06960"
                     /db_xref="CDD:143518"
     Site            order(59,62,76,79,95,101,111,114)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143518"
     Site            order(65,69..71,77..78,80,82,84..85,88,108..109,112,115,
                     128..130,132)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143518"
     Site            order(96,109..110,112)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:143518"
     Region          153..372
                     /region_name="NR_LBD_HNF4_like"
                     /note="The ligand binding domain of heptocyte nuclear
                     factor 4, which is explosively expanded in nematodes;
                     cd06931"
                     /db_xref="CDD:132729"
     Site            order(184,190,194,228,232,235,245..246,263,353..354,357)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132729"
     Site            order(199,203,213..214,216..217,220..221,367..368,
                     371..372)
                     /site_type="other"
                     /note="coactivator recognition site [polypeptide binding]"
                     /db_xref="CDD:132729"
     Site            order(270,291,297,311..312,315..316,319..320,330..331,
                     333..335,337..338,340..342,344..345,348)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:132729"
     CDS             1..476
                     /gene="Hnf4"
                     /coded_by="XM_077646931.1:113..1543"
                     /db_xref="GeneID:144113687"
ORIGIN      
        1 mnaepsgasa gllgcaaplg svivqggppa maidasgqds elayssqqsg tpsvlsqfca
       61 icgdratgkh ygaascdgck gffrrsvrkn hlyscrfnrs cvvdkdkrnq crycrlkkcf
      121 ragmkkeavq nerdrisnrr psyeesnnss glsvtnllna emysrnissp vseynisnkk
      181 ianisdvces mkqqllilvd waksvpsfte lsiedqvall rahagehlll glarrsmhtk
      241 nvlllgndfi iprvstevei srigarimde lvttlrevhi ddtefaclra ivffdphtkg
      301 lsepqkikal rhqvqtnled yindrqyesr grfgeimlll ptlqsitwqm ieqiqfaklf
      361 gmakvdnllq emllggmvgp qafynqgssp dcpsgastsp lslsssvihq silqsnagie
      421 nltngrrcdg nelgassrpq pdtpipsppd ptgfkpmqdl gvqafktevl etdaty
//
LOCUS       XP_077503058             978 aa            linear   INV 25-NOV-2025
DEFINITION  pre-mRNA processing factor 4 kinase isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503058
VERSION     XP_077503058.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646932.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..978
                     /product="pre-mRNA processing factor 4 kinase isoform X1"
                     /calculated_mol_wt=111457
     Region          158..>398
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     Region          657..974
                     /region_name="STKc_PRP4"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Pre-mRNA-Processing factor 4; cd14135"
                     /db_xref="CDD:271037"
     Site            order(664..668,672,686,688,702,722,738..741,744,746..747,
                     786,788,790..791,793,805,808,818,820..823,825,862)
                     /site_type="active"
                     /db_xref="CDD:271037"
     Site            order(664..668,672,686,688,722,738..741,744,786,788,
                     790..791,793,805)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271037"
     Site            order(702,746,786,788,808,818,820..823,825,862)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271037"
     Site            804..825
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271037"
     CDS             1..978
                     /gene="Prp4k"
                     /coded_by="XM_077646932.1:245..3181"
                     /db_xref="GeneID:144113688"
ORIGIN      
        1 mlpstkamer sscaasnmlg gcpaakllad deqvaepark egkeggskhk krkhkhkhkh
       61 hhssskdksg krkssssgkt alpidtesnq aalqvssmnl eeleeqkail qaklytlvps
      121 dygssdeevg kskkskvssr shessskksh tslldhkaer egksrehsrs ehrkerdgsp
      181 rssrkahiek tqkdtstspt lkrsapkres pssrhrkspk rspsrrsspp rksplrklsp
      241 irktspsrrl spshkstspv rrsspsrkts plrraspsrk vspprrmspw kvspsrrssp
      301 prkvsparrv spsrrvsplr rvsplrrssp srkgsplrka spsrkaspsr rlspprfrae
      361 lerwrsaspr rtgdrhvprs psprypsrrl spprnsgrea grdmnrdvrr dsreagrers
      421 prrarprspl rrrsghsrsp rrgdfrsltl rsrsphgmgr nddrgrhgrd hwdrrddrgr
      481 hanssrkkpk spssssdeie dieieedede ekvierqrqq rqellkrlgv enqvessgfs
      541 spvhsagsgt htpdsvtidn matdmnense dtadfeaaim akrqmivqeg ntesdtgqek
      601 kkqldmfsed mfaenyssps svqkissraq enpnltdnwd daegyyrvri gevldtrytv
      661 ygytgqgvfs nvvrardmar gnqdvavkii rnnevmhrtg lkelemlrrl ndadpddkfh
      721 clrlfrhffh kqhlclvfep lsmnlrevlk kygkdvglhi kavrsysqql ffalkllkrc
      781 nilhadikpd nilvnetklv lklcdfgsas hvaenditpy lvsrfyrape ivlglgydfg
      841 idmwsvgctl yelytgkimf pgksnnqmlk fcmdlkgkfp nrlirkgifr dqhfdancnf
      901 lyhevdkvte rekvvvmtni nqtrdlstel igrqrlpedq vkkvgqlkdl lektlmldpa
      961 krisinhala hpfihdki
//
LOCUS       XP_077503059             978 aa            linear   INV 25-NOV-2025
DEFINITION  pre-mRNA processing factor 4 kinase isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503059
VERSION     XP_077503059.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646933.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..978
                     /product="pre-mRNA processing factor 4 kinase isoform X1"
                     /calculated_mol_wt=111457
     Region          158..>398
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     Region          657..974
                     /region_name="STKc_PRP4"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Pre-mRNA-Processing factor 4; cd14135"
                     /db_xref="CDD:271037"
     Site            order(664..668,672,686,688,702,722,738..741,744,746..747,
                     786,788,790..791,793,805,808,818,820..823,825,862)
                     /site_type="active"
                     /db_xref="CDD:271037"
     Site            order(664..668,672,686,688,722,738..741,744,786,788,
                     790..791,793,805)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271037"
     Site            order(702,746,786,788,808,818,820..823,825,862)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271037"
     Site            804..825
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271037"
     CDS             1..978
                     /gene="Prp4k"
                     /coded_by="XM_077646933.1:180..3116"
                     /db_xref="GeneID:144113688"
ORIGIN      
        1 mlpstkamer sscaasnmlg gcpaakllad deqvaepark egkeggskhk krkhkhkhkh
       61 hhssskdksg krkssssgkt alpidtesnq aalqvssmnl eeleeqkail qaklytlvps
      121 dygssdeevg kskkskvssr shessskksh tslldhkaer egksrehsrs ehrkerdgsp
      181 rssrkahiek tqkdtstspt lkrsapkres pssrhrkspk rspsrrsspp rksplrklsp
      241 irktspsrrl spshkstspv rrsspsrkts plrraspsrk vspprrmspw kvspsrrssp
      301 prkvsparrv spsrrvsplr rvsplrrssp srkgsplrka spsrkaspsr rlspprfrae
      361 lerwrsaspr rtgdrhvprs psprypsrrl spprnsgrea grdmnrdvrr dsreagrers
      421 prrarprspl rrrsghsrsp rrgdfrsltl rsrsphgmgr nddrgrhgrd hwdrrddrgr
      481 hanssrkkpk spssssdeie dieieedede ekvierqrqq rqellkrlgv enqvessgfs
      541 spvhsagsgt htpdsvtidn matdmnense dtadfeaaim akrqmivqeg ntesdtgqek
      601 kkqldmfsed mfaenyssps svqkissraq enpnltdnwd daegyyrvri gevldtrytv
      661 ygytgqgvfs nvvrardmar gnqdvavkii rnnevmhrtg lkelemlrrl ndadpddkfh
      721 clrlfrhffh kqhlclvfep lsmnlrevlk kygkdvglhi kavrsysqql ffalkllkrc
      781 nilhadikpd nilvnetklv lklcdfgsas hvaenditpy lvsrfyrape ivlglgydfg
      841 idmwsvgctl yelytgkimf pgksnnqmlk fcmdlkgkfp nrlirkgifr dqhfdancnf
      901 lyhevdkvte rekvvvmtni nqtrdlstel igrqrlpedq vkkvgqlkdl lektlmldpa
      961 krisinhala hpfihdki
//
LOCUS       XP_077503060             978 aa            linear   INV 25-NOV-2025
DEFINITION  pre-mRNA processing factor 4 kinase isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503060
VERSION     XP_077503060.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646934.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..978
                     /product="pre-mRNA processing factor 4 kinase isoform X1"
                     /calculated_mol_wt=111457
     Region          158..>398
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     Region          657..974
                     /region_name="STKc_PRP4"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Pre-mRNA-Processing factor 4; cd14135"
                     /db_xref="CDD:271037"
     Site            order(664..668,672,686,688,702,722,738..741,744,746..747,
                     786,788,790..791,793,805,808,818,820..823,825,862)
                     /site_type="active"
                     /db_xref="CDD:271037"
     Site            order(664..668,672,686,688,722,738..741,744,786,788,
                     790..791,793,805)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271037"
     Site            order(702,746,786,788,808,818,820..823,825,862)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271037"
     Site            804..825
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271037"
     CDS             1..978
                     /gene="Prp4k"
                     /coded_by="XM_077646934.1:245..3181"
                     /db_xref="GeneID:144113688"
ORIGIN      
        1 mlpstkamer sscaasnmlg gcpaakllad deqvaepark egkeggskhk krkhkhkhkh
       61 hhssskdksg krkssssgkt alpidtesnq aalqvssmnl eeleeqkail qaklytlvps
      121 dygssdeevg kskkskvssr shessskksh tslldhkaer egksrehsrs ehrkerdgsp
      181 rssrkahiek tqkdtstspt lkrsapkres pssrhrkspk rspsrrsspp rksplrklsp
      241 irktspsrrl spshkstspv rrsspsrkts plrraspsrk vspprrmspw kvspsrrssp
      301 prkvsparrv spsrrvsplr rvsplrrssp srkgsplrka spsrkaspsr rlspprfrae
      361 lerwrsaspr rtgdrhvprs psprypsrrl spprnsgrea grdmnrdvrr dsreagrers
      421 prrarprspl rrrsghsrsp rrgdfrsltl rsrsphgmgr nddrgrhgrd hwdrrddrgr
      481 hanssrkkpk spssssdeie dieieedede ekvierqrqq rqellkrlgv enqvessgfs
      541 spvhsagsgt htpdsvtidn matdmnense dtadfeaaim akrqmivqeg ntesdtgqek
      601 kkqldmfsed mfaenyssps svqkissraq enpnltdnwd daegyyrvri gevldtrytv
      661 ygytgqgvfs nvvrardmar gnqdvavkii rnnevmhrtg lkelemlrrl ndadpddkfh
      721 clrlfrhffh kqhlclvfep lsmnlrevlk kygkdvglhi kavrsysqql ffalkllkrc
      781 nilhadikpd nilvnetklv lklcdfgsas hvaenditpy lvsrfyrape ivlglgydfg
      841 idmwsvgctl yelytgkimf pgksnnqmlk fcmdlkgkfp nrlirkgifr dqhfdancnf
      901 lyhevdkvte rekvvvmtni nqtrdlstel igrqrlpedq vkkvgqlkdl lektlmldpa
      961 krisinhala hpfihdki
//
LOCUS       XP_077503061             964 aa            linear   INV 25-NOV-2025
DEFINITION  pre-mRNA processing factor 4 kinase isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503061
VERSION     XP_077503061.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646935.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..964
                     /product="pre-mRNA processing factor 4 kinase isoform X2"
                     /calculated_mol_wt=110314
     Region          144..>384
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     Region          643..960
                     /region_name="STKc_PRP4"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Pre-mRNA-Processing factor 4; cd14135"
                     /db_xref="CDD:271037"
     Site            order(650..654,658,672,674,688,708,724..727,730,732..733,
                     772,774,776..777,779,791,794,804,806..809,811,848)
                     /site_type="active"
                     /db_xref="CDD:271037"
     Site            order(650..654,658,672,674,708,724..727,730,772,774,
                     776..777,779,791)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271037"
     Site            order(688,732,772,774,794,804,806..809,811,848)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271037"
     Site            790..811
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271037"
     CDS             1..964
                     /gene="Prp4k"
                     /coded_by="XM_077646935.1:109..3003"
                     /db_xref="GeneID:144113688"
ORIGIN      
        1 mqfdamvplk kdrsesdeqv aeparkegke ggskhkkrkh khkhkhhhss skdksgkrks
       61 sssgktalpi dtesnqaalq vssmnleele eqkailqakl ytlvpsdygs sdeevgkskk
      121 skvssrshes sskkshtsll dhkaeregks rehsrsehrk erdgsprssr kahiektqkd
      181 tstsptlkrs apkrespssr hrkspkrsps rrsspprksp lrklspirkt spsrrlspsh
      241 kstspvrrss psrktsplrr aspsrkvspp rrmspwkvsp srrsspprkv sparrvspsr
      301 rvsplrrvsp lrrsspsrkg splrkaspsr kaspsrrlsp prfraelerw rsasprrtgd
      361 rhvprspspr ypsrrlsppr nsgreagrdm nrdvrrdsre agrersprra rprsplrrrs
      421 ghsrsprrgd frsltlrsrs phgmgrnddr grhgrdhwdr rddrgrhans srkkpkspss
      481 ssdeiediei eededeekvi erqrqqrqel lkrlgvenqv essgfsspvh sagsgthtpd
      541 svtidnmatd mnensedtad feaaimakrq mivqegntes dtgqekkkql dmfsedmfae
      601 nysspssvqk issraqenpn ltdnwddaeg yyrvrigevl dtrytvygyt gqgvfsnvvr
      661 ardmargnqd vavkiirnne vmhrtglkel emlrrlndad pddkfhclrl frhffhkqhl
      721 clvfeplsmn lrevlkkygk dvglhikavr sysqqlffal kllkrcnilh adikpdnilv
      781 netklvlklc dfgsashvae nditpylvsr fyrapeivlg lgydfgidmw svgctlyely
      841 tgkimfpgks nnqmlkfcmd lkgkfpnrli rkgifrdqhf dancnflyhe vdkvterekv
      901 vvmtninqtr dlsteligrq rlpedqvkkv gqlkdllekt lmldpakris inhalahpfi
      961 hdki
//
LOCUS       XP_077503062             964 aa            linear   INV 25-NOV-2025
DEFINITION  pre-mRNA processing factor 4 kinase isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503062
VERSION     XP_077503062.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646936.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..964
                     /product="pre-mRNA processing factor 4 kinase isoform X2"
                     /calculated_mol_wt=110314
     Region          144..>384
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     Region          643..960
                     /region_name="STKc_PRP4"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Pre-mRNA-Processing factor 4; cd14135"
                     /db_xref="CDD:271037"
     Site            order(650..654,658,672,674,688,708,724..727,730,732..733,
                     772,774,776..777,779,791,794,804,806..809,811,848)
                     /site_type="active"
                     /db_xref="CDD:271037"
     Site            order(650..654,658,672,674,708,724..727,730,772,774,
                     776..777,779,791)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271037"
     Site            order(688,732,772,774,794,804,806..809,811,848)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271037"
     Site            790..811
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271037"
     CDS             1..964
                     /gene="Prp4k"
                     /coded_by="XM_077646936.1:106..3000"
                     /db_xref="GeneID:144113688"
ORIGIN      
        1 mqfdamvplk kdrsesdeqv aeparkegke ggskhkkrkh khkhkhhhss skdksgkrks
       61 sssgktalpi dtesnqaalq vssmnleele eqkailqakl ytlvpsdygs sdeevgkskk
      121 skvssrshes sskkshtsll dhkaeregks rehsrsehrk erdgsprssr kahiektqkd
      181 tstsptlkrs apkrespssr hrkspkrsps rrsspprksp lrklspirkt spsrrlspsh
      241 kstspvrrss psrktsplrr aspsrkvspp rrmspwkvsp srrsspprkv sparrvspsr
      301 rvsplrrvsp lrrsspsrkg splrkaspsr kaspsrrlsp prfraelerw rsasprrtgd
      361 rhvprspspr ypsrrlsppr nsgreagrdm nrdvrrdsre agrersprra rprsplrrrs
      421 ghsrsprrgd frsltlrsrs phgmgrnddr grhgrdhwdr rddrgrhans srkkpkspss
      481 ssdeiediei eededeekvi erqrqqrqel lkrlgvenqv essgfsspvh sagsgthtpd
      541 svtidnmatd mnensedtad feaaimakrq mivqegntes dtgqekkkql dmfsedmfae
      601 nysspssvqk issraqenpn ltdnwddaeg yyrvrigevl dtrytvygyt gqgvfsnvvr
      661 ardmargnqd vavkiirnne vmhrtglkel emlrrlndad pddkfhclrl frhffhkqhl
      721 clvfeplsmn lrevlkkygk dvglhikavr sysqqlffal kllkrcnilh adikpdnilv
      781 netklvlklc dfgsashvae nditpylvsr fyrapeivlg lgydfgidmw svgctlyely
      841 tgkimfpgks nnqmlkfcmd lkgkfpnrli rkgifrdqhf dancnflyhe vdkvterekv
      901 vvmtninqtr dlsteligrq rlpedqvkkv gqlkdllekt lmldpakris inhalahpfi
      961 hdki
//
LOCUS       XP_077503063             973 aa            linear   INV 25-NOV-2025
DEFINITION  pre-mRNA processing factor 4 kinase isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503063
VERSION     XP_077503063.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646937.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..973
                     /product="pre-mRNA processing factor 4 kinase isoform X3"
                     /calculated_mol_wt=111114
     Region          153..>393
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     Region          652..969
                     /region_name="STKc_PRP4"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Pre-mRNA-Processing factor 4; cd14135"
                     /db_xref="CDD:271037"
     Site            order(659..663,667,681,683,697,717,733..736,739,741..742,
                     781,783,785..786,788,800,803,813,815..818,820,857)
                     /site_type="active"
                     /db_xref="CDD:271037"
     Site            order(659..663,667,681,683,717,733..736,739,781,783,
                     785..786,788,800)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271037"
     Site            order(697,741,781,783,803,813,815..818,820,857)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271037"
     Site            799..820
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271037"
     CDS             1..973
                     /gene="Prp4k"
                     /coded_by="XM_077646937.1:155..3076"
                     /db_xref="GeneID:144113688"
ORIGIN      
        1 makpcvqstv rsvdsmqdvk ngdvsdeqva eparkegkeg gskhkkrkhk hkhkhhhsss
       61 kdksgkrkss ssgktalpid tesnqaalqv ssmnleelee qkailqakly tlvpsdygss
      121 deevgkskks kvssrshess skkshtslld hkaeregksr ehsrsehrke rdgsprssrk
      181 ahiektqkdt stsptlkrsa pkrespssrh rkspkrspsr rsspprkspl rklspirkts
      241 psrrlspshk stspvrrssp srktsplrra spsrkvsppr rmspwkvsps rrsspprkvs
      301 parrvspsrr vsplrrvspl rrsspsrkgs plrkaspsrk aspsrrlspp rfraelerwr
      361 sasprrtgdr hvprspspry psrrlspprn sgreagrdmn rdvrrdsrea grersprrar
      421 prsplrrrsg hsrsprrgdf rsltlrsrsp hgmgrnddrg rhgrdhwdrr ddrgrhanss
      481 rkkpkspsss sdeiedieie ededeekvie rqrqqrqell krlgvenqve ssgfsspvhs
      541 agsgthtpds vtidnmatdm nensedtadf eaaimakrqm ivqegntesd tgqekkkqld
      601 mfsedmfaen ysspssvqki ssraqenpnl tdnwddaegy yrvrigevld trytvygytg
      661 qgvfsnvvra rdmargnqdv avkiirnnev mhrtglkele mlrrlndadp ddkfhclrlf
      721 rhffhkqhlc lvfeplsmnl revlkkygkd vglhikavrs ysqqlffalk llkrcnilha
      781 dikpdnilvn etklvlklcd fgsashvaen ditpylvsrf yrapeivlgl gydfgidmws
      841 vgctlyelyt gkimfpgksn nqmlkfcmdl kgkfpnrlir kgifrdqhfd ancnflyhev
      901 dkvterekvv vmtninqtrd lsteligrqr lpedqvkkvg qlkdllektl mldpakrisi
      961 nhalahpfih dki
//
LOCUS       XP_077503065             318 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113690 [Amblyomma americanum].
ACCESSION   XP_077503065
VERSION     XP_077503065.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646939.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..318
                     /product="uncharacterized protein LOC144113690"
                     /calculated_mol_wt=35481
     Region          5..95
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..318
                     /gene="LOC144113690"
                     /coded_by="XM_077646939.1:55..1011"
                     /db_xref="GeneID:144113690"
ORIGIN      
        1 mpgcccapgc rsnydggpsv rvykfpadet qraawtkais redfvptkht vvcekhfqts
       61 dfvrfatytd entgnvievp lqrvrlkpaa vpsvfpgcpk ylsqppssre apaekkarle
      121 aaslrkaiel svaaqeeedr rnkicsfgel csalpklkts dfwtvvttqa kvlflelsld
      181 qapavrssvv vlddlsvqvf lggsrlhnlg decvpsqlkd lreldkvlqs veklqscasg
      241 sekehqllat vlmlldevnk dyelqehhgw nlqvlkfvrs qvelvlknvp ryprdvlvfs
      301 gllyhhfstc iqdhqkla
//
LOCUS       XP_077503066             533 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113691 [Amblyomma americanum].
ACCESSION   XP_077503066
VERSION     XP_077503066.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646940.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..533
                     /product="uncharacterized protein LOC144113691"
                     /calculated_mol_wt=58633
     Region          13..111
                     /region_name="MULE"
                     /note="MULE transposase domain; pfam10551"
                     /db_xref="CDD:463145"
     CDS             1..533
                     /gene="LOC144113691"
                     /coded_by="XM_077646940.1:35..1636"
                     /db_xref="GeneID:144113691"
ORIGIN      
        1 mkrtqglsaa keiifvdsta scdesqsslt vvltatavga mplavllhss qssesykaaf
       61 gllklsyptc fggthapqaf mtdnsaaeka alqatwpqgk qllchfhvaq aewrwlhasr
      121 dtsrddkrdl mttfqkimya standlntai aemkakshkg yvarvlgfle rkaewvllyr
      181 sgmltrghnt nnfaeasirv lkdvvlgrhk afnvvalvdl itslweghlq krllkhaynr
      241 vpshkllydk llermpenaa asirtlgsnl fevpsckedg klyevwqdvg tctcragqqg
      301 afckhqalvh stygggfpna pvlstqdrhq lawlalgdkc qnpaffrdfr esawdqpgss
      361 sgepgnepvq pqpqplvqda tslpqdtmev eeqlavagps snhqcredaa evlkniteel
      421 wrqyslaadn pfyltllqrd lallkrarte pqvvamhlas taakasslrr griikvqptg
      481 larrrpgvtr gaarvhagrp lktagsrktk rlhslhlsvk davphakshg tgh
//
LOCUS       XP_077503067             725 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial proton/calcium exchanger protein-like [Amblyomma
            americanum].
ACCESSION   XP_077503067
VERSION     XP_077503067.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646941.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..725
                     /product="mitochondrial proton/calcium exchanger
                     protein-like"
                     /calculated_mol_wt=82544
     Region          161..383
                     /region_name="LETM1_RBD"
                     /note="LETM1-like, RBD; pfam07766"
                     /db_xref="CDD:462258"
     CDS             1..725
                     /gene="LOC144113692"
                     /coded_by="XM_077646941.1:335..2512"
                     /db_xref="GeneID:144113692"
ORIGIN      
        1 mhrrpiscsl ylwqlsmlrs grcvaagfhr swppyrhvss swhgctpllh rqrtppagtv
       61 pshsvlslvl crplslpsqr lfekdsskve gtvkalkeae pstvaakpdt patpapvppk
      121 rplikriwde lvhyyhgfrl lfidirvssr lvyrvlngee ltrrehkqlv rtvsdlfrll
      181 pfsvfiivpf melllpvalk lfpnmlpstf qtssakdakv kkelkvklem akflqstlde
      241 mavkkrgeah shnakefakf cekiresged atneeilkfs klfedeitld sltrpqltal
      301 crllelqpig tnnflrfqlr mklrnlkadd qmiqkegies ltvaelqavc rargmramgl
      361 pesklryqla qwldlslnen ippsllllsr amllsetlpp teqlkatist lpkeavteak
      421 ykigeregkv dnrtkieiiq qeeeaikkek eeiavevete klkavadqkp eeeikeiled
      481 kaaffteqde dlekkppvpk keelskadla diedalekia aekqelliek eeledlkeem
      541 adykqdvdel kdvvvktgkr diqetkaamr lynrvnriig smdhlidkln eerksmqikl
      601 dstegaasek tkdnlieind lvlamrriqk sssdtrlemi tdlldtldid hdgavkidhv
      661 lkvidtlgre nvkvspaqmk eiiellikee vlqaknkeqq aastdaaaqp pssehkeqqh
      721 enknt
//
LOCUS       XP_077503068             121 aa            linear   INV 25-NOV-2025
DEFINITION  crustacean cardioactive peptide [Amblyomma americanum].
ACCESSION   XP_077503068
VERSION     XP_077503068.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646942.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..121
                     /product="crustacean cardioactive peptide"
                     /calculated_mol_wt=13415
     Region          2..>66
                     /region_name="CCAP"
                     /note="Arthropod cardioacceleratory peptide 2a; pfam11105"
                     /db_xref="CDD:314123"
     CDS             1..121
                     /gene="CCAP"
                     /coded_by="XM_077646942.1:117..482"
                     /db_xref="GeneID:144113693"
ORIGIN      
        1 msatglflfc alvfsasltv aqekpdeatd erfspeqkrp fcnaftgcgg krsqyrtrnl
       61 lsrlrqrvln eaarsqqryr hqfaedvgsr ggfdvddlrn gailvnlpgl lrkrraalvg
      121 p
//
LOCUS       XP_077503069             121 aa            linear   INV 25-NOV-2025
DEFINITION  crustacean cardioactive peptide [Amblyomma americanum].
ACCESSION   XP_077503069
VERSION     XP_077503069.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646943.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..121
                     /product="crustacean cardioactive peptide"
                     /calculated_mol_wt=13415
     Region          2..>66
                     /region_name="CCAP"
                     /note="Arthropod cardioacceleratory peptide 2a; pfam11105"
                     /db_xref="CDD:314123"
     CDS             1..121
                     /gene="CCAP"
                     /coded_by="XM_077646943.1:203..568"
                     /db_xref="GeneID:144113693"
ORIGIN      
        1 msatglflfc alvfsasltv aqekpdeatd erfspeqkrp fcnaftgcgg krsqyrtrnl
       61 lsrlrqrvln eaarsqqryr hqfaedvgsr ggfdvddlrn gailvnlpgl lrkrraalvg
      121 p
//
LOCUS       XP_077503070             110 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113695 [Amblyomma americanum].
ACCESSION   XP_077503070
VERSION     XP_077503070.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646944.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..110
                     /product="uncharacterized protein LOC144113695"
                     /calculated_mol_wt=11675
     CDS             1..110
                     /gene="LOC144113695"
                     /coded_by="XM_077646944.1:359..691"
                     /db_xref="GeneID:144113695"
ORIGIN      
        1 msrlsrfggg sclcpfqrtl qstsvsefkv ekavygtkkg ipkdavfhkr aattiwstea
       61 vaqcsssskc sggdsgrywa tqlessccqg narfstfsek mhatmqgasa
//
LOCUS       XP_077503071             110 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113695 [Amblyomma americanum].
ACCESSION   XP_077503071
VERSION     XP_077503071.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646945.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..110
                     /product="uncharacterized protein LOC144113695"
                     /calculated_mol_wt=11675
     CDS             1..110
                     /gene="LOC144113695"
                     /coded_by="XM_077646945.1:438..770"
                     /db_xref="GeneID:144113695"
ORIGIN      
        1 msrlsrfggg sclcpfqrtl qstsvsefkv ekavygtkkg ipkdavfhkr aattiwstea
       61 vaqcsssskc sggdsgrywa tqlessccqg narfstfsek mhatmqgasa
//
LOCUS       XP_077503072             110 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113695 [Amblyomma americanum].
ACCESSION   XP_077503072
VERSION     XP_077503072.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646946.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..110
                     /product="uncharacterized protein LOC144113695"
                     /calculated_mol_wt=11675
     CDS             1..110
                     /gene="LOC144113695"
                     /coded_by="XM_077646946.1:358..690"
                     /db_xref="GeneID:144113695"
ORIGIN      
        1 msrlsrfggg sclcpfqrtl qstsvsefkv ekavygtkkg ipkdavfhkr aattiwstea
       61 vaqcsssskc sggdsgrywa tqlessccqg narfstfsek mhatmqgasa
//
LOCUS       XP_077503074             110 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113695 [Amblyomma americanum].
ACCESSION   XP_077503074
VERSION     XP_077503074.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646948.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..110
                     /product="uncharacterized protein LOC144113695"
                     /calculated_mol_wt=11675
     CDS             1..110
                     /gene="LOC144113695"
                     /coded_by="XM_077646948.1:191..523"
                     /db_xref="GeneID:144113695"
ORIGIN      
        1 msrlsrfggg sclcpfqrtl qstsvsefkv ekavygtkkg ipkdavfhkr aattiwstea
       61 vaqcsssskc sggdsgrywa tqlessccqg narfstfsek mhatmqgasa
//
LOCUS       XP_077503075             110 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113696 [Amblyomma americanum].
ACCESSION   XP_077503075
VERSION     XP_077503075.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646949.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..110
                     /product="uncharacterized protein LOC144113696"
                     /calculated_mol_wt=11675
     CDS             1..110
                     /gene="LOC144113696"
                     /coded_by="XM_077646949.1:363..695"
                     /db_xref="GeneID:144113696"
ORIGIN      
        1 msrlsrfggg sclcpfqrtl qstsvsefkv ekavygtkkg ipkdavfhkr aattiwstea
       61 vaqcsssskc sggdsgrywa tqlessccqg narfstfsek mhatmqgasa
//
LOCUS       XP_077503076             110 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113696 [Amblyomma americanum].
ACCESSION   XP_077503076
VERSION     XP_077503076.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646950.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..110
                     /product="uncharacterized protein LOC144113696"
                     /calculated_mol_wt=11675
     CDS             1..110
                     /gene="LOC144113696"
                     /coded_by="XM_077646950.1:199..531"
                     /db_xref="GeneID:144113696"
ORIGIN      
        1 msrlsrfggg sclcpfqrtl qstsvsefkv ekavygtkkg ipkdavfhkr aattiwstea
       61 vaqcsssskc sggdsgrywa tqlessccqg narfstfsek mhatmqgasa
//
LOCUS       XP_077503077             110 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113696 [Amblyomma americanum].
ACCESSION   XP_077503077
VERSION     XP_077503077.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646951.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..110
                     /product="uncharacterized protein LOC144113696"
                     /calculated_mol_wt=11675
     CDS             1..110
                     /gene="LOC144113696"
                     /coded_by="XM_077646951.1:180..512"
                     /db_xref="GeneID:144113696"
ORIGIN      
        1 msrlsrfggg sclcpfqrtl qstsvsefkv ekavygtkkg ipkdavfhkr aattiwstea
       61 vaqcsssskc sggdsgrywa tqlessccqg narfstfsek mhatmqgasa
//
LOCUS       XP_077503078             110 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113696 [Amblyomma americanum].
ACCESSION   XP_077503078
VERSION     XP_077503078.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646952.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..110
                     /product="uncharacterized protein LOC144113696"
                     /calculated_mol_wt=11675
     CDS             1..110
                     /gene="LOC144113696"
                     /coded_by="XM_077646952.1:76..408"
                     /db_xref="GeneID:144113696"
ORIGIN      
        1 msrlsrfggg sclcpfqrtl qstsvsefkv ekavygtkkg ipkdavfhkr aattiwstea
       61 vaqcsssskc sggdsgrywa tqlessccqg narfstfsek mhatmqgasa
//
LOCUS       XP_077503079             110 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113697 [Amblyomma americanum].
ACCESSION   XP_077503079
VERSION     XP_077503079.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646953.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..110
                     /product="uncharacterized protein LOC144113697"
                     /calculated_mol_wt=11661
     CDS             1..110
                     /gene="LOC144113697"
                     /coded_by="XM_077646953.1:335..667"
                     /db_xref="GeneID:144113697"
ORIGIN      
        1 msrlsrfggg sclcpfqrsl qstsvsefkv ekavygtkkg ipkdavfhkr aattiwstea
       61 vaqcsssskc sggdsgrywa tqlessccqg narfstfsek mhatmqgasa
//
LOCUS       XP_077503080             110 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113697 [Amblyomma americanum].
ACCESSION   XP_077503080
VERSION     XP_077503080.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646954.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..110
                     /product="uncharacterized protein LOC144113697"
                     /calculated_mol_wt=11661
     CDS             1..110
                     /gene="LOC144113697"
                     /coded_by="XM_077646954.1:431..763"
                     /db_xref="GeneID:144113697"
ORIGIN      
        1 msrlsrfggg sclcpfqrsl qstsvsefkv ekavygtkkg ipkdavfhkr aattiwstea
       61 vaqcsssskc sggdsgrywa tqlessccqg narfstfsek mhatmqgasa
//
LOCUS       XP_077503081             672 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase AMFR-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503081
VERSION     XP_077503081.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646955.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..672
                     /product="E3 ubiquitin-protein ligase AMFR-like isoform
                     X1"
                     /calculated_mol_wt=76565
     Region          97..>399
                     /region_name="HRD1"
                     /note="HRD ubiquitin ligase complex, ER membrane component
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG5243"
                     /db_xref="CDD:227568"
     Region          356..399
                     /region_name="RING-H2_AMFR"
                     /note="RING finger, H2 subclass, found in autocrine
                     motility factor receptor (AMFR) and similar proteins;
                     cd16455"
                     /db_xref="CDD:438119"
     Site            order(359..363,384..385,388..389,393..394,396)
                     /site_type="other"
                     /note="E2 binding site [polypeptide binding]"
                     /db_xref="CDD:438119"
     Region          481..521
                     /region_name="CUE_AMFR"
                     /note="CUE domain found in autocrine motility factor
                     receptor (AMFR) and similar proteins; cd14421"
                     /db_xref="CDD:270604"
     Region          614..637
                     /region_name="G2BR"
                     /note="E3 gp78 Ube2g2-binding region (G2BR); pfam18442"
                     /db_xref="CDD:375868"
     CDS             1..672
                     /gene="LOC144113698"
                     /coded_by="XM_077646955.1:197..2215"
                     /db_xref="GeneID:144113698"
ORIGIN      
        1 mpailldrlp lpslqaytvi svlllsgsvy yavqvtsqle wkinatfnfd fttdidttmt
       61 vgnltfvsnh qvtleqflle hhcvrrllei vyfmfqeplc vwtlinmayc cliligkviq
      121 klvfgelrvs eqqhmkdkfw nfvfykfifi fgvmnvqymd evvlwcswfs vlgflhllaq
      181 lckdrfeyls fspttpklth irllallcgi lllslglfav cvvvglhaga ntfafmaaec
      241 slvtvrtlyv ivrygihlwd ihcdkvwenr aayvyyaelc feltalaidf chdlhmllwg
      301 niflsmaslv ilmqlrylfy eiqrrvkkhk nylrvvkhme anypmatade leknsddcai
      361 cwdhmesark lpcghlfhns clrswleqdt scptcrmsls epgalagesl qdegrnttll
      421 lgalggqedt prhpqnattn hffhfdgsry vswlpsfsve vthtnllggq psppvqtsql
      481 dnmarqvqqm fpsmplntiv edlrstrsve ltienilder liapptsmyq tavprnsrgt
      541 vynsgfqnhg ldthkealad erfprnssan vldstspmfl edpepypqae eastsgdnsm
      601 gpqvggrfsk spserermls rrkeellkma rkkfldrska gpsvqnirla selssmslsq
      661 phrrlrqphl gs
//
LOCUS       XP_077503082             672 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase AMFR-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503082
VERSION     XP_077503082.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646956.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..672
                     /product="E3 ubiquitin-protein ligase AMFR-like isoform
                     X1"
                     /calculated_mol_wt=76565
     Region          97..>399
                     /region_name="HRD1"
                     /note="HRD ubiquitin ligase complex, ER membrane component
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG5243"
                     /db_xref="CDD:227568"
     Region          356..399
                     /region_name="RING-H2_AMFR"
                     /note="RING finger, H2 subclass, found in autocrine
                     motility factor receptor (AMFR) and similar proteins;
                     cd16455"
                     /db_xref="CDD:438119"
     Site            order(359..363,384..385,388..389,393..394,396)
                     /site_type="other"
                     /note="E2 binding site [polypeptide binding]"
                     /db_xref="CDD:438119"
     Region          481..521
                     /region_name="CUE_AMFR"
                     /note="CUE domain found in autocrine motility factor
                     receptor (AMFR) and similar proteins; cd14421"
                     /db_xref="CDD:270604"
     Region          614..637
                     /region_name="G2BR"
                     /note="E3 gp78 Ube2g2-binding region (G2BR); pfam18442"
                     /db_xref="CDD:375868"
     CDS             1..672
                     /gene="LOC144113698"
                     /coded_by="XM_077646956.1:257..2275"
                     /db_xref="GeneID:144113698"
ORIGIN      
        1 mpailldrlp lpslqaytvi svlllsgsvy yavqvtsqle wkinatfnfd fttdidttmt
       61 vgnltfvsnh qvtleqflle hhcvrrllei vyfmfqeplc vwtlinmayc cliligkviq
      121 klvfgelrvs eqqhmkdkfw nfvfykfifi fgvmnvqymd evvlwcswfs vlgflhllaq
      181 lckdrfeyls fspttpklth irllallcgi lllslglfav cvvvglhaga ntfafmaaec
      241 slvtvrtlyv ivrygihlwd ihcdkvwenr aayvyyaelc feltalaidf chdlhmllwg
      301 niflsmaslv ilmqlrylfy eiqrrvkkhk nylrvvkhme anypmatade leknsddcai
      361 cwdhmesark lpcghlfhns clrswleqdt scptcrmsls epgalagesl qdegrnttll
      421 lgalggqedt prhpqnattn hffhfdgsry vswlpsfsve vthtnllggq psppvqtsql
      481 dnmarqvqqm fpsmplntiv edlrstrsve ltienilder liapptsmyq tavprnsrgt
      541 vynsgfqnhg ldthkealad erfprnssan vldstspmfl edpepypqae eastsgdnsm
      601 gpqvggrfsk spserermls rrkeellkma rkkfldrska gpsvqnirla selssmslsq
      661 phrrlrqphl gs
//
LOCUS       XP_077503083             590 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase AMFR-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503083
VERSION     XP_077503083.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646957.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..590
                     /product="E3 ubiquitin-protein ligase AMFR-like isoform
                     X2"
                     /calculated_mol_wt=66964
     Region          22..>317
                     /region_name="HRD1"
                     /note="HRD ubiquitin ligase complex, ER membrane component
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG5243"
                     /db_xref="CDD:227568"
     Region          274..317
                     /region_name="RING-H2_AMFR"
                     /note="RING finger, H2 subclass, found in autocrine
                     motility factor receptor (AMFR) and similar proteins;
                     cd16455"
                     /db_xref="CDD:438119"
     Site            order(277..281,302..303,306..307,311..312,314)
                     /site_type="other"
                     /note="E2 binding site [polypeptide binding]"
                     /db_xref="CDD:438119"
     Region          399..439
                     /region_name="CUE_AMFR"
                     /note="CUE domain found in autocrine motility factor
                     receptor (AMFR) and similar proteins; cd14421"
                     /db_xref="CDD:270604"
     Region          532..555
                     /region_name="G2BR"
                     /note="E3 gp78 Ube2g2-binding region (G2BR); pfam18442"
                     /db_xref="CDD:375868"
     CDS             1..590
                     /gene="LOC144113698"
                     /coded_by="XM_077646957.1:660..2432"
                     /db_xref="GeneID:144113698"
ORIGIN      
        1 mepaivavas cfdrandvyk tlinmayccl iligkviqkl vfgelrvseq qhmkdkfwnf
       61 vfykfififg vmnvqymdev vlwcswfsvl gflhllaqlc kdrfeylsfs pttpklthir
      121 llallcgill lslglfavcv vvglhagant fafmaaecsl vtvrtlyviv rygihlwdih
      181 cdkvwenraa yvyyaelcfe ltalaidfch dlhmllwgni flsmaslvil mqlrylfyei
      241 qrrvkkhkny lrvvkhmean ypmatadele knsddcaicw dhmesarklp cghlfhnscl
      301 rswleqdtsc ptcrmslsep galageslqd egrnttlllg alggqedtpr hpqnattnhf
      361 fhfdgsryvs wlpsfsvevt htnllggqps ppvqtsqldn marqvqqmfp smplntived
      421 lrstrsvelt ienilderli apptsmyqta vprnsrgtvy nsgfqnhgld thkealader
      481 fprnssanvl dstspmfled pepypqaeea stsgdnsmgp qvggrfsksp serermlsrr
      541 keellkmark kfldrskagp svqnirlase lssmslsqph rrlrqphlgs
//
LOCUS       XP_077503084             296 aa            linear   INV 25-NOV-2025
DEFINITION  cytoplasmic dynein 2 intermediate chain 2-like [Amblyomma
            americanum].
ACCESSION   XP_077503084
VERSION     XP_077503084.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646958.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..296
                     /product="cytoplasmic dynein 2 intermediate chain 2-like"
                     /calculated_mol_wt=32118
     Region          <151..>296
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          154..191
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          197..235
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..296
                     /gene="LOC144113700"
                     /coded_by="XM_077646958.1:186..1076"
                     /db_xref="GeneID:144113700"
ORIGIN      
        1 mwdveaaneq wasspvvmhd nhqdmvkgve wigdaqephc llscaldgli iqwrhdarkn
       61 qleassyfll laenvrlhkk pisgtttayi gvtslaknhf dhsvfvmgte gggvlqgsls
      121 lskpiapvvd shasvertys cpvvarfpph rghtldihss pfernvfasa gidrevkvfs
      181 llqteavlsv qlpepvsrvr wspsqptlfa alqgdgrlaf ydlrrttapv aefapdghna
      241 tgtslefctr srdllfagrg pgtaqvwqlg srlvaasean qgdlirsvan gsfvpd
//
LOCUS       XP_077503085             208 aa            linear   INV 25-NOV-2025
DEFINITION  DNA replication complex GINS protein Psf3 [Amblyomma americanum].
ACCESSION   XP_077503085
VERSION     XP_077503085.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646959.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..208
                     /product="DNA replication complex GINS protein Psf3"
                     /calculated_mol_wt=23491
     Region          16..79
                     /region_name="GINS_B_Psf3"
                     /note="beta-strand (B) domain of GINS complex protein
                     Psf3; cd21693"
                     /db_xref="CDD:412029"
     Site            order(16..17,19,21..23,25..26,37..40,63..64,67..68,73)
                     /site_type="other"
                     /note="tetramer interface [polypeptide binding]"
                     /db_xref="CDD:412029"
     Region          73..182
                     /region_name="GINS_A_psf3"
                     /note="Alpha-helical domain of GINS complex protein Psf3
                     (partner of Sld5 3); cd11713"
                     /db_xref="CDD:212551"
     Site            order(108,111,132,136,140..141,158,161,163,165..175)
                     /site_type="other"
                     /note="tetramer interface [polypeptide binding]"
                     /db_xref="CDD:212551"
     CDS             1..208
                     /gene="Psf3"
                     /coded_by="XM_077646959.1:99..725"
                     /db_xref="GeneID:144113701"
ORIGIN      
        1 mssasgsrrv pcfgdyfsld dilttnervp ckveilipkl gslntsskee dlklgtkldl
       61 pywlaaplls rrivsaevpk vyretyreil sadavavdls rlqpqyygfg lliqklplsd
      121 veyvnnslik afknrfrhim dcsqgsfked lldltarldm seralfalgr galedlqrwq
      181 rrqshrista amvanhrkrk raaltdsv
//
LOCUS       XP_077503087            1152 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113702 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503087
VERSION     XP_077503087.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646961.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1152
                     /product="uncharacterized protein LOC144113702 isoform X1"
                     /calculated_mol_wt=125908
     Region          1021..1140
                     /region_name="ALMS_motif"
                     /note="ALMS motif; pfam15309"
                     /db_xref="CDD:464634"
     CDS             1..1152
                     /gene="LOC144113702"
                     /coded_by="XM_077646961.1:238..3696"
                     /db_xref="GeneID:144113702"
ORIGIN      
        1 mvfatcelge melpltaqss flsafsfplc ystpdaqsds dtsahcedql pilrtiwstt
       61 ncsdssacan ggrrsrttiy qytplsvpsp pqftssaheq elknacvgfa lstfprtakk
      121 dsmettdgcp pgktqgisys appalmfaas rgtsvassfs sesipvvtts qgstehesss
      181 ytvsgpadgg tqsydkmlee eqvagsapfc prvrsanqsp tesaldavvt akrdvsegnl
      241 msnpelalsl esvltvvpqs lrqssaagea eepegkqfik eqflkkpscd vsawkpryst
      301 sscgdvsfqs wdshleidta iedivdsvih elticetdfh scsdtfsrgd stvdiprrvs
      361 paslssytss krlewdngad igysgasert qrherlqypd ieshihrsnt svlineasvs
      421 peekirmfag phfrdvvvqt eacsikdaev qanasatnlv stqsgatast ertdllvsgs
      481 gtedlnglap trrehslssh aeddhrknyn erevpftavg hmcakqkpas rgsfevpmqd
      541 yassvslcys hrilkeasnl kkhassvrkh aadnngqver rhrdfvtapd apdidsssld
      601 vtgftiinnn rlkmrfptav pglasrsatp aeppracsel ykrrldywls sgfpyvpavv
      661 pletdalpri rtadpatakq dcrdprlvvs gsslpsltqc qkatnmtrae pagtatgadg
      721 mvrkryiarp aplkvsktsr sdavlslqas apcqkllrqd klgigvptkp slvmndihsg
      781 tpslsgaaks tllsrghsed allsrsnese vcsspellva srkqqvcgrh ssstsystas
      841 egtssqlltv ngkscklkrl ahkeleklrf lvdrqrhgyl kqlqreverl hrleqlflsa
      901 agsrtasspe aqttstekns tedacritdr nsvavqtsqf flenchsedd vakdrcfvsv
      961 sggagvkkqr drlsprkrkp tskrpvawvv pfgsnsqysg skhrgsspkg ksqqlshkee
     1021 slqaafaahc srlikrsear qskvaslaer rrmqrsvlpq aqsqernpth shppgtrgga
     1081 ptkktfthke mreqtervyq klpevidskk rlhreqqyrt nrlmaqlykk aiqekallgr
     1141 vnfplnkpft qp
//
LOCUS       XP_077503088            1142 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113702 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503088
VERSION     XP_077503088.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646962.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1142
                     /product="uncharacterized protein LOC144113702 isoform X2"
                     /calculated_mol_wt=124827
     Region          1011..1130
                     /region_name="ALMS_motif"
                     /note="ALMS motif; pfam15309"
                     /db_xref="CDD:464634"
     CDS             1..1142
                     /gene="LOC144113702"
                     /coded_by="XM_077646962.1:113..3541"
                     /db_xref="GeneID:144113702"
ORIGIN      
        1 melpltaqss flsafsfplc ystpdaqsds dtsahcedql pilrtiwstt ncsdssacan
       61 ggrrsrttiy qytplsvpsp pqftssaheq elknacvgfa lstfprtakk dsmettdgcp
      121 pgktqgisys appalmfaas rgtsvassfs sesipvvtts qgstehesss ytvsgpadgg
      181 tqsydkmlee eqvagsapfc prvrsanqsp tesaldavvt akrdvsegnl msnpelalsl
      241 esvltvvpqs lrqssaagea eepegkqfik eqflkkpscd vsawkpryst sscgdvsfqs
      301 wdshleidta iedivdsvih elticetdfh scsdtfsrgd stvdiprrvs paslssytss
      361 krlewdngad igysgasert qrherlqypd ieshihrsnt svlineasvs peekirmfag
      421 phfrdvvvqt eacsikdaev qanasatnlv stqsgatast ertdllvsgs gtedlnglap
      481 trrehslssh aeddhrknyn erevpftavg hmcakqkpas rgsfevpmqd yassvslcys
      541 hrilkeasnl kkhassvrkh aadnngqver rhrdfvtapd apdidsssld vtgftiinnn
      601 rlkmrfptav pglasrsatp aeppracsel ykrrldywls sgfpyvpavv pletdalpri
      661 rtadpatakq dcrdprlvvs gsslpsltqc qkatnmtrae pagtatgadg mvrkryiarp
      721 aplkvsktsr sdavlslqas apcqkllrqd klgigvptkp slvmndihsg tpslsgaaks
      781 tllsrghsed allsrsnese vcsspellva srkqqvcgrh ssstsystas egtssqlltv
      841 ngkscklkrl ahkeleklrf lvdrqrhgyl kqlqreverl hrleqlflsa agsrtasspe
      901 aqttstekns tedacritdr nsvavqtsqf flenchsedd vakdrcfvsv sggagvkkqr
      961 drlsprkrkp tskrpvawvv pfgsnsqysg skhrgsspkg ksqqlshkee slqaafaahc
     1021 srlikrsear qskvaslaer rrmqrsvlpq aqsqernpth shppgtrgga ptkktfthke
     1081 mreqtervyq klpevidskk rlhreqqyrt nrlmaqlykk aiqekallgr vnfplnkpft
     1141 qp
//
LOCUS       XP_077503089            1142 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113702 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503089
VERSION     XP_077503089.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646963.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1142
                     /product="uncharacterized protein LOC144113702 isoform X2"
                     /calculated_mol_wt=124827
     Region          1011..1130
                     /region_name="ALMS_motif"
                     /note="ALMS motif; pfam15309"
                     /db_xref="CDD:464634"
     CDS             1..1142
                     /gene="LOC144113702"
                     /coded_by="XM_077646963.1:117..3545"
                     /db_xref="GeneID:144113702"
ORIGIN      
        1 melpltaqss flsafsfplc ystpdaqsds dtsahcedql pilrtiwstt ncsdssacan
       61 ggrrsrttiy qytplsvpsp pqftssaheq elknacvgfa lstfprtakk dsmettdgcp
      121 pgktqgisys appalmfaas rgtsvassfs sesipvvtts qgstehesss ytvsgpadgg
      181 tqsydkmlee eqvagsapfc prvrsanqsp tesaldavvt akrdvsegnl msnpelalsl
      241 esvltvvpqs lrqssaagea eepegkqfik eqflkkpscd vsawkpryst sscgdvsfqs
      301 wdshleidta iedivdsvih elticetdfh scsdtfsrgd stvdiprrvs paslssytss
      361 krlewdngad igysgasert qrherlqypd ieshihrsnt svlineasvs peekirmfag
      421 phfrdvvvqt eacsikdaev qanasatnlv stqsgatast ertdllvsgs gtedlnglap
      481 trrehslssh aeddhrknyn erevpftavg hmcakqkpas rgsfevpmqd yassvslcys
      541 hrilkeasnl kkhassvrkh aadnngqver rhrdfvtapd apdidsssld vtgftiinnn
      601 rlkmrfptav pglasrsatp aeppracsel ykrrldywls sgfpyvpavv pletdalpri
      661 rtadpatakq dcrdprlvvs gsslpsltqc qkatnmtrae pagtatgadg mvrkryiarp
      721 aplkvsktsr sdavlslqas apcqkllrqd klgigvptkp slvmndihsg tpslsgaaks
      781 tllsrghsed allsrsnese vcsspellva srkqqvcgrh ssstsystas egtssqlltv
      841 ngkscklkrl ahkeleklrf lvdrqrhgyl kqlqreverl hrleqlflsa agsrtasspe
      901 aqttstekns tedacritdr nsvavqtsqf flenchsedd vakdrcfvsv sggagvkkqr
      961 drlsprkrkp tskrpvawvv pfgsnsqysg skhrgsspkg ksqqlshkee slqaafaahc
     1021 srlikrsear qskvaslaer rrmqrsvlpq aqsqernpth shppgtrgga ptkktfthke
     1081 mreqtervyq klpevidskk rlhreqqyrt nrlmaqlykk aiqekallgr vnfplnkpft
     1141 qp
//
LOCUS       XP_077503090            1142 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113702 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503090
VERSION     XP_077503090.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646964.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1142
                     /product="uncharacterized protein LOC144113702 isoform X2"
                     /calculated_mol_wt=124827
     Region          1011..1130
                     /region_name="ALMS_motif"
                     /note="ALMS motif; pfam15309"
                     /db_xref="CDD:464634"
     CDS             1..1142
                     /gene="LOC144113702"
                     /coded_by="XM_077646964.1:165..3593"
                     /db_xref="GeneID:144113702"
ORIGIN      
        1 melpltaqss flsafsfplc ystpdaqsds dtsahcedql pilrtiwstt ncsdssacan
       61 ggrrsrttiy qytplsvpsp pqftssaheq elknacvgfa lstfprtakk dsmettdgcp
      121 pgktqgisys appalmfaas rgtsvassfs sesipvvtts qgstehesss ytvsgpadgg
      181 tqsydkmlee eqvagsapfc prvrsanqsp tesaldavvt akrdvsegnl msnpelalsl
      241 esvltvvpqs lrqssaagea eepegkqfik eqflkkpscd vsawkpryst sscgdvsfqs
      301 wdshleidta iedivdsvih elticetdfh scsdtfsrgd stvdiprrvs paslssytss
      361 krlewdngad igysgasert qrherlqypd ieshihrsnt svlineasvs peekirmfag
      421 phfrdvvvqt eacsikdaev qanasatnlv stqsgatast ertdllvsgs gtedlnglap
      481 trrehslssh aeddhrknyn erevpftavg hmcakqkpas rgsfevpmqd yassvslcys
      541 hrilkeasnl kkhassvrkh aadnngqver rhrdfvtapd apdidsssld vtgftiinnn
      601 rlkmrfptav pglasrsatp aeppracsel ykrrldywls sgfpyvpavv pletdalpri
      661 rtadpatakq dcrdprlvvs gsslpsltqc qkatnmtrae pagtatgadg mvrkryiarp
      721 aplkvsktsr sdavlslqas apcqkllrqd klgigvptkp slvmndihsg tpslsgaaks
      781 tllsrghsed allsrsnese vcsspellva srkqqvcgrh ssstsystas egtssqlltv
      841 ngkscklkrl ahkeleklrf lvdrqrhgyl kqlqreverl hrleqlflsa agsrtasspe
      901 aqttstekns tedacritdr nsvavqtsqf flenchsedd vakdrcfvsv sggagvkkqr
      961 drlsprkrkp tskrpvawvv pfgsnsqysg skhrgsspkg ksqqlshkee slqaafaahc
     1021 srlikrsear qskvaslaer rrmqrsvlpq aqsqernpth shppgtrgga ptkktfthke
     1081 mreqtervyq klpevidskk rlhreqqyrt nrlmaqlykk aiqekallgr vnfplnkpft
     1141 qp
//
LOCUS       XP_077503091            1142 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113702 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503091
VERSION     XP_077503091.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646965.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1142
                     /product="uncharacterized protein LOC144113702 isoform X2"
                     /calculated_mol_wt=124827
     Region          1011..1130
                     /region_name="ALMS_motif"
                     /note="ALMS motif; pfam15309"
                     /db_xref="CDD:464634"
     CDS             1..1142
                     /gene="LOC144113702"
                     /coded_by="XM_077646965.1:147..3575"
                     /db_xref="GeneID:144113702"
ORIGIN      
        1 melpltaqss flsafsfplc ystpdaqsds dtsahcedql pilrtiwstt ncsdssacan
       61 ggrrsrttiy qytplsvpsp pqftssaheq elknacvgfa lstfprtakk dsmettdgcp
      121 pgktqgisys appalmfaas rgtsvassfs sesipvvtts qgstehesss ytvsgpadgg
      181 tqsydkmlee eqvagsapfc prvrsanqsp tesaldavvt akrdvsegnl msnpelalsl
      241 esvltvvpqs lrqssaagea eepegkqfik eqflkkpscd vsawkpryst sscgdvsfqs
      301 wdshleidta iedivdsvih elticetdfh scsdtfsrgd stvdiprrvs paslssytss
      361 krlewdngad igysgasert qrherlqypd ieshihrsnt svlineasvs peekirmfag
      421 phfrdvvvqt eacsikdaev qanasatnlv stqsgatast ertdllvsgs gtedlnglap
      481 trrehslssh aeddhrknyn erevpftavg hmcakqkpas rgsfevpmqd yassvslcys
      541 hrilkeasnl kkhassvrkh aadnngqver rhrdfvtapd apdidsssld vtgftiinnn
      601 rlkmrfptav pglasrsatp aeppracsel ykrrldywls sgfpyvpavv pletdalpri
      661 rtadpatakq dcrdprlvvs gsslpsltqc qkatnmtrae pagtatgadg mvrkryiarp
      721 aplkvsktsr sdavlslqas apcqkllrqd klgigvptkp slvmndihsg tpslsgaaks
      781 tllsrghsed allsrsnese vcsspellva srkqqvcgrh ssstsystas egtssqlltv
      841 ngkscklkrl ahkeleklrf lvdrqrhgyl kqlqreverl hrleqlflsa agsrtasspe
      901 aqttstekns tedacritdr nsvavqtsqf flenchsedd vakdrcfvsv sggagvkkqr
      961 drlsprkrkp tskrpvawvv pfgsnsqysg skhrgsspkg ksqqlshkee slqaafaahc
     1021 srlikrsear qskvaslaer rrmqrsvlpq aqsqernpth shppgtrgga ptkktfthke
     1081 mreqtervyq klpevidskk rlhreqqyrt nrlmaqlykk aiqekallgr vnfplnkpft
     1141 qp
//
LOCUS       XP_077503092            1028 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113703 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503092
VERSION     XP_077503092.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646966.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1028
                     /product="uncharacterized protein LOC144113703 isoform X1"
                     /calculated_mol_wt=112741
     Region          961..1016
                     /region_name="FAM193_C"
                     /note="FAM193 family C-terminal; pfam15914"
                     /db_xref="CDD:464940"
     CDS             1..1028
                     /gene="LOC144113703"
                     /coded_by="XM_077646966.1:117..3203"
                     /db_xref="GeneID:144113703"
ORIGIN      
        1 mspaetkktk rrknkkvarp tgkeqiegvg msagvgsanp cigqsakdag vhdltlsvsp
       61 acgpkelqgc ilcsrahstd satehtkdav difgpeavwd slpsdalcss dalfsedidl
      121 ssalrdgtfp kellsladdc gssvcvcery snwkalepea dqqellcwqv vkqalhrkyq
      181 raglgseavp plqddqplps daevramvsr lrrrdpqhll qclkyqaqlf vvesrlrmlr
      241 qlvtgprlad alvaeyaklr lawegldpll eelvtldlnk yglswelvnk ylfqaivysd
      301 peihkcltdl lthrhsikfk qdhkalleld ndmtlttiiw rkvqqlldep hqleqvlpaq
      361 wmspswkpvd pdlvaknlgq ccrsllagel qqegcnsale qrqcclatas gmvcpfaetr
      421 psvlaageps stsplsqspv lpgaawghgg dtcgpgldsq sseaslpkka aetcechact
      481 qmpwnpqsll qlygsrplnl dldsldslpq lrdwedghfk srhtstdlsp qpappgttsp
      541 qssstparrh sepgtcldsd cdnalvdged slddscsehs ssttasnqrs agenrvcdcc
      601 ycevfghgap pvapvsknyq emrdrlrlil skrknqlrga dhhgsgavrq qrqrmqaeai
      661 hmakqrpqqh hhqerheddr eveellsyin gtdsggsrke sahvtmdanv tsttsspgvk
      721 gtvtkrhkrk tkrredldkv nkaieikvhs apvisrlved aeeskvpssp kavvnrkear
      781 kengvrlskc pksngastng ssciasihel neecpppevs lcnrvtsstm istlsnsrvp
      841 ataisgnvfi igrptlpset gsmldeetvs kmtigdvels vtsttlssnn vlktvcqdrq
      901 asmpeasssc nhrqsrardq lseakkqlci thhlngtqta sknkksrkkk nssadvtspd
      961 evflpkdidl engdldeler eveafkrfcf nsvpvankek vhvnlkdill rrkqtsttvi
     1021 pagllqrt
//
LOCUS       XP_077503093            1006 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113703 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503093
VERSION     XP_077503093.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646967.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1006
                     /product="uncharacterized protein LOC144113703 isoform X2"
                     /calculated_mol_wt=110229
     Region          939..994
                     /region_name="FAM193_C"
                     /note="FAM193 family C-terminal; pfam15914"
                     /db_xref="CDD:464940"
     CDS             1..1006
                     /gene="LOC144113703"
                     /coded_by="XM_077646967.1:127..3147"
                     /db_xref="GeneID:144113703"
ORIGIN      
        1 mspaetkktk rrknkkvarp tgkeqiegvg msagvgsanp cigqsakdag vhdltlsvsp
       61 acgpkelqgc ilcsrahstd satehtkdav difgpeavwd slpsdalcss dalfsedidl
      121 ssalrdgtfp kellsladdc gssvcvcery snwkalepea dqqellcwqv vkqalhrkyq
      181 raglgseavp plqddqplps daevramvsr lrrrdpqhll qclkyqaqlf vvesrlrmlr
      241 qlvtgprlad alvaeyaklr lawegldpll eelvtldlnk yglswelvnk ylfqaivysd
      301 peihkcltdl lthrhsikfk qdhkalleld ndmtlttiiw rkvqqlldep hqleqvlpaq
      361 wmspswkpvd pdlvaknlgq ccrsllagel qqegcnsale qrqcclatas gmvcpfaetr
      421 psvlaageps stsplsqspv lpgaawghgg dtcgpgldsq sseaslpkka aetcechact
      481 qmpwnpqsll qlygsrplnl dldsldslpq lrdwedghfk srhtstdlsp qpappgttsp
      541 qssstparrh sepgtcldsd cdnalvdged slddscsehs ssttasnqrs agenrvcdcc
      601 ycevfghgap pvapvsknyq emrdrlrlil skrkaeaihm akqrpqqhhh qerheddrev
      661 eellsyingt dsggsrkesa hvtmdanvts ttsspgvkgt vtkrhkrktk rredldkvnk
      721 aieikvhsap visrlvedae eskvpsspka vvnrkearke ngvrlskcpk sngastngss
      781 ciasihelne ecpppevslc nrvtsstmis tlsnsrvpat aisgnvfiig rptlpsetgs
      841 mldeetvskm tigdvelsvt sttlssnnvl ktvcqdrqas mpeassscnh rqsrardqls
      901 eakkqlcith hlngtqtask nkksrkkkns sadvtspdev flpkdidlen gdldelerev
      961 eafkrfcfns vpvankekvh vnlkdillrr kqtsttvipa gllqrt
//
LOCUS       XP_077503094             958 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113703 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503094
VERSION     XP_077503094.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646968.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..958
                     /product="uncharacterized protein LOC144113703 isoform X3"
                     /calculated_mol_wt=104546
     Region          891..946
                     /region_name="FAM193_C"
                     /note="FAM193 family C-terminal; pfam15914"
                     /db_xref="CDD:464940"
     CDS             1..958
                     /gene="LOC144113703"
                     /coded_by="XM_077646968.1:117..2993"
                     /db_xref="GeneID:144113703"
ORIGIN      
        1 mspaetkktk rrknkkvarp tgkeqiegvg msagvgsanp cigqsakdag vhdltlsvsp
       61 acgpkelqgc ilcsrahstd satehtkdav difgpeavwd slpsdalcss dalfsedidl
      121 ssalrdgtfp kellsladdc gssvcvcery snwkalepea dqqellcwqv vkqalhrkyq
      181 raglgseavp plqddqplps daevtldlnk yglswelvnk ylfqaivysd peihkcltdl
      241 lthrhsikfk qdhkalleld ndmtlttiiw rkvqqlldep hqleqvlpaq wmspswkpvd
      301 pdlvaknlgq ccrsllagel qqegcnsale qrqcclatas gmvcpfaetr psvlaageps
      361 stsplsqspv lpgaawghgg dtcgpgldsq sseaslpkka aetcechact qmpwnpqsll
      421 qlygsrplnl dldsldslpq lrdwedghfk srhtstdlsp qpappgttsp qssstparrh
      481 sepgtcldsd cdnalvdged slddscsehs ssttasnqrs agenrvcdcc ycevfghgap
      541 pvapvsknyq emrdrlrlil skrknqlrga dhhgsgavrq qrqrmqaeai hmakqrpqqh
      601 hhqerheddr eveellsyin gtdsggsrke sahvtmdanv tsttsspgvk gtvtkrhkrk
      661 tkrredldkv nkaieikvhs apvisrlved aeeskvpssp kavvnrkear kengvrlskc
      721 pksngastng ssciasihel neecpppevs lcnrvtsstm istlsnsrvp ataisgnvfi
      781 igrptlpset gsmldeetvs kmtigdvels vtsttlssnn vlktvcqdrq asmpeasssc
      841 nhrqsrardq lseakkqlci thhlngtqta sknkksrkkk nssadvtspd evflpkdidl
      901 engdldeler eveafkrfcf nsvpvankek vhvnlkdill rrkqtsttvi pagllqrt
//
LOCUS       XP_077503095             947 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113703 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503095
VERSION     XP_077503095.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646969.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..947
                     /product="uncharacterized protein LOC144113703 isoform X4"
                     /calculated_mol_wt=103100
     Region          880..935
                     /region_name="FAM193_C"
                     /note="FAM193 family C-terminal; pfam15914"
                     /db_xref="CDD:464940"
     CDS             1..947
                     /gene="LOC144113703"
                     /coded_by="XM_077646969.1:127..2970"
                     /db_xref="GeneID:144113703"
ORIGIN      
        1 mspaetkktk rrknkkvarp tgkeqiegvg msagvgsanp cigqsakdag vhdltlsvsp
       61 acgpkelqgc ilcsrahstd satehtkdav difgpeavwd slpsdalcss dalfsedidl
      121 ssalrdgtfp kellsladdc gssvcvcery snwkalepea dqqellcwqv vkqalhrkyq
      181 raglgseavp plqddqplps daevramvsr lrrrdpqhll qclkyqaqlf vvesrlrmlr
      241 qlvtgprlad alvaeyaklr lawegldpll eelqvlpaqw mspswkpvdp dlvaknlgqc
      301 crsllagelq qegcnsaleq rqcclatasg mvcpfaetrp svlaagepss tsplsqspvl
      361 pgaawghggd tcgpgldsqs seaslpkkaa etcechactq mpwnpqsllq lygsrplnld
      421 ldsldslpql rdwedghfks rhtstdlspq pappgttspq ssstparrhs epgtcldsdc
      481 dnalvdgeds lddscsehss sttasnqrsa genrvcdccy cevfghgapp vapvsknyqe
      541 mrdrlrlils krknqlrgad hhgsgavrqq rqrmqaeaih makqrpqqhh hqerheddre
      601 veellsying tdsggsrkes ahvtmdanvt sttsspgvkg tvtkrhkrkt krredldkvn
      661 kaieikvhsa pvisrlveda eeskvpsspk avvnrkeark engvrlskcp ksngastngs
      721 sciasiheln eecpppevsl cnrvtsstmi stlsnsrvpa taisgnvfii grptlpsetg
      781 smldeetvsk mtigdvelsv tsttlssnnv lktvcqdrqa smpeassscn hrqsrardql
      841 seakkqlcit hhlngtqtas knkksrkkkn ssadvtspde vflpkdidle ngdldelere
      901 veafkrfcfn svpvankekv hvnlkdillr rkqtsttvip agllqrt
//
LOCUS       XP_077503096             925 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113703 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077503096
VERSION     XP_077503096.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646970.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..925
                     /product="uncharacterized protein LOC144113703 isoform X5"
                     /calculated_mol_wt=100588
     Region          858..913
                     /region_name="FAM193_C"
                     /note="FAM193 family C-terminal; pfam15914"
                     /db_xref="CDD:464940"
     CDS             1..925
                     /gene="LOC144113703"
                     /coded_by="XM_077646970.1:117..2894"
                     /db_xref="GeneID:144113703"
ORIGIN      
        1 mspaetkktk rrknkkvarp tgkeqiegvg msagvgsanp cigqsakdag vhdltlsvsp
       61 acgpkelqgc ilcsrahstd satehtkdav difgpeavwd slpsdalcss dalfsedidl
      121 ssalrdgtfp kellsladdc gssvcvcery snwkalepea dqqellcwqv vkqalhrkyq
      181 raglgseavp plqddqplps daevramvsr lrrrdpqhll qclkyqaqlf vvesrlrmlr
      241 qlvtgprlad alvaeyaklr lawegldpll eelqvlpaqw mspswkpvdp dlvaknlgqc
      301 crsllagelq qegcnsaleq rqcclatasg mvcpfaetrp svlaagepss tsplsqspvl
      361 pgaawghggd tcgpgldsqs seaslpkkaa etcechactq mpwnpqsllq lygsrplnld
      421 ldsldslpql rdwedghfks rhtstdlspq pappgttspq ssstparrhs epgtcldsdc
      481 dnalvdgeds lddscsehss sttasnqrsa genrvcdccy cevfghgapp vapvsknyqe
      541 mrdrlrlils krkaeaihma kqrpqqhhhq erheddreve ellsyingtd sggsrkesah
      601 vtmdanvtst tsspgvkgtv tkrhkrktkr redldkvnka ieikvhsapv isrlvedaee
      661 skvpsspkav vnrkearken gvrlskcpks ngastngssc iasihelnee cpppevslcn
      721 rvtsstmist lsnsrvpata isgnvfiigr ptlpsetgsm ldeetvskmt igdvelsvts
      781 ttlssnnvlk tvcqdrqasm peassscnhr qsrardqlse akkqlcithh lngtqtaskn
      841 kksrkkknss advtspdevf lpkdidleng dldelereve afkrfcfnsv pvankekvhv
      901 nlkdillrrk qtsttvipag llqrt
//
LOCUS       XP_077503097             798 aa            linear   INV 25-NOV-2025
DEFINITION  neprilysin-1-like [Amblyomma americanum].
ACCESSION   XP_077503097
VERSION     XP_077503097.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646971.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..798
                     /product="neprilysin-1-like"
                     /calculated_mol_wt=91331
     Region          137..795
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..798
                     /gene="LOC144113706"
                     /coded_by="XM_077646971.1:20..2416"
                     /db_xref="GeneID:144113706"
ORIGIN      
        1 mepspsalyq eggvrstspe tkccavrtrs rtihtshsrl ayvvvctvgv vmvlvgvavs
       61 tyklqngsti lelfnrtmsl ftynataagq ddseeaahps rhllkhhvkl pdadttctte
      121 tclwmerylr gklntsvnpc vdfysyvcss kwfskgldir drpymertvg mlmldvekff
      181 raylkqneen yhkypgvfvh qaisllskcq seekpdknft alrslleeyn lggwpyrrap
      241 rgvnivtisa fvdrdlgvfs farvylkkpf eddrgytvhl dkpsstlerh qlaflsesve
      301 nytnrvelal slfetkhdms eqaeaivale qklaaampvp kftefqdrik rvghldrrgk
      361 wdwkeylnii fqdievfdne kpvaimhhdy llkmasivne aetvtllnyi gyrlvvqvsp
      421 llakpasqll rmshdnyqef vperlqacmh llervyrqgm rfftrmtfsk dnstlmlkhy
      481 dysmsnveae ikssmadrll stsswldraa igigvdklen mrlvflgssd nintvanyyn
      541 fnaqpldpth llesfreiqa atmsiywetk ppkddldary dhstltpgye yffgrnvlfv
      601 phgniafvnd isknidpvly plllpnvlrg mfsavdrrga tvdhnsavst wwntdelskf
      661 sqielcfqdq yyielrklvg dsfdarikld eniadsaila plhdlyvktl tsqgttldkq
      721 klpvdigevd mhkmffisya lglcdnpske ssvrkvkyer ipgrlrinip lmnfakfsta
      781 fncpvnspmn parkcavw
//
LOCUS       XP_077503098             270 aa            linear   INV 25-NOV-2025
DEFINITION  transmembrane protein 127-like [Amblyomma americanum].
ACCESSION   XP_077503098
VERSION     XP_077503098.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646972.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..270
                     /product="transmembrane protein 127-like"
                     /calculated_mol_wt=30209
     Region          100..193
                     /region_name="TMEM127"
                     /note="Transmembrane protein 127; pfam20517"
                     /db_xref="CDD:466666"
     CDS             1..270
                     /gene="LOC144113707"
                     /coded_by="XM_077646972.1:257..1069"
                     /db_xref="GeneID:144113707"
ORIGIN      
        1 mpleratarh hrphhwrlke rernllaavl sivviatlst alvhprwfyl qgggcghkyl
       61 gvqnffyvgy feafprlfvn gpsrlpsyiy ygwnevmkdc vtptivslqr vlivlcflai
      121 lssllqffmd algvtykwlr virrnafcsi vtvglclgvv gtcyyistlm evqqemtkps
      181 rssrvevsfs vsyhlvaaag aaavlaagan llsrpvpvtq lgdsaidsll ldddfsretf
      241 vvgishsemw pyrqdltrlh slpplppysp
//
LOCUS       XP_077503099             270 aa            linear   INV 25-NOV-2025
DEFINITION  transmembrane protein 127-like [Amblyomma americanum].
ACCESSION   XP_077503099
VERSION     XP_077503099.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646973.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..270
                     /product="transmembrane protein 127-like"
                     /calculated_mol_wt=30209
     Region          100..193
                     /region_name="TMEM127"
                     /note="Transmembrane protein 127; pfam20517"
                     /db_xref="CDD:466666"
     CDS             1..270
                     /gene="LOC144113707"
                     /coded_by="XM_077646973.1:335..1147"
                     /db_xref="GeneID:144113707"
ORIGIN      
        1 mpleratarh hrphhwrlke rernllaavl sivviatlst alvhprwfyl qgggcghkyl
       61 gvqnffyvgy feafprlfvn gpsrlpsyiy ygwnevmkdc vtptivslqr vlivlcflai
      121 lssllqffmd algvtykwlr virrnafcsi vtvglclgvv gtcyyistlm evqqemtkps
      181 rssrvevsfs vsyhlvaaag aaavlaagan llsrpvpvtq lgdsaidsll ldddfsretf
      241 vvgishsemw pyrqdltrlh slpplppysp
//
LOCUS       XP_077503100             800 aa            linear   INV 25-NOV-2025
DEFINITION  neprilysin-1-like [Amblyomma americanum].
ACCESSION   XP_077503100
VERSION     XP_077503100.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646974.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..800
                     /product="neprilysin-1-like"
                     /calculated_mol_wt=91565
     Region          139..797
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..800
                     /gene="LOC144113708"
                     /coded_by="XM_077646974.1:948..3350"
                     /db_xref="GeneID:144113708"
ORIGIN      
        1 mepspsalyq eggvrttsse tkccvvrtrt rsrtihtshs rlayvvvctv gvvmvlvgia
       61 vstyklhngs tvlelfnrpm slfiynataa gqddseeaah psrhllkhhv klpdadttct
      121 tetclwmery lrgklntsvn pcvdfysyvc sskwfskgld irdrpymert vgmlmldvek
      181 ffraylkqne enyhnypgvf vhqaisllsk cqseekpdkn ftalrsllee ynlggwpyrr
      241 aprgvnivti safvdrdlgv fsfarvylkk pfeddrgytv hldkpsstle rhqlaflses
      301 venytnrvdl alslfetkhd mseqaeaiva leqklsaamp vpkftefqdr ikrvghldrr
      361 gkwdwkeyln iifqdievfe nekpvaimhq dyllkmasiv netepvtlln yigyrlvvqv
      421 spllakpagq llrmshdnyq efvperlqac mhllervyrq gmrfftrmtf skenstlllk
      481 hydysmsnve vqikssmadr llstsswldr aaigigvdkl enmrlvflgs sddintvany
      541 ynfnaqpldp thllesfrev qaatmsiywe tkspkddlda rydhstltpg yeyffgrnvl
      601 fvphgniafv ndisknidpv lyplllpnvl rgmfsavdrr gatvdhnsav stwwntdels
      661 kfsqielcfq dqyyielrkl vgdsfdarik ldeniadsai laplhdlyvk tltsqgttld
      721 kqklpvdige vdmhkmffis yalglcdnps kessvrkvky elipgrlrin iplinfakfs
      781 tafncpvnsp mnparkcavw
//
LOCUS       XP_077503101             519 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113709 [Amblyomma americanum].
ACCESSION   XP_077503101
VERSION     XP_077503101.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646975.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..519
                     /product="uncharacterized protein LOC144113709"
                     /calculated_mol_wt=56845
     Region          40..175
                     /region_name="PAAT-like"
                     /note="ATPase PAAT-like; pfam14958"
                     /db_xref="CDD:464404"
     CDS             1..519
                     /gene="LOC144113709"
                     /coded_by="XM_077646975.1:300..1859"
                     /db_xref="GeneID:144113709"
ORIGIN      
        1 mikakctwkv qgdrpvsdai vyfednenid ledaisaenc vqleapvndd vtgnyivpac
       61 qvflfcgdth qigsvsilse arvievygyh geylstlkne lmeevdgmsv frgdltlarp
      121 lkecclkfip lkskdsmwly gikvvvqekp rpdtlnlfpt tmplkdveer leasgaqlse
      181 kaenlkrmme lfqgstsmls aaamspvlag fsalprgtpq qvpdgmtqly nlfqnaslks
      241 pqvqqvpakq vpkptvtqep tretvpqagl gavetsqdgl lagmlklmle nqtikqqpqq
      301 epqpqpqvvt nakaseqqsa sldkefilgl lersgvlnkq tatallteii gktgatvqln
      361 qsfgdageks tnvpaedaia gqskivaekk ilqttsiate tehelskeaa sqtdvpepvq
      421 panealqaip aqlavealqi qreevrdavg tqfqglqdql lnlvgqrfeq lkaemfekle
      481 gkiqemeeri narfdaiida lqgqeddeei feqpeegsv
//
LOCUS       XP_077503102             731 aa            linear   INV 25-NOV-2025
DEFINITION  DEAH-box helicase 15 [Amblyomma americanum].
ACCESSION   XP_077503102
VERSION     XP_077503102.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646976.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..731
                     /product="DEAH-box helicase 15"
                     /calculated_mol_wt=83778
     Region          72..>698
                     /region_name="HrpA"
                     /note="HrpA-like RNA helicase [Translation, ribosomal
                     structure and biogenesis]; COG1643"
                     /db_xref="CDD:441249"
     CDS             1..731
                     /gene="Dhx15"
                     /coded_by="XM_077646976.1:122..2317"
                     /db_xref="GeneID:144113710"
ORIGIN      
        1 mskrhridvg espmtkkrdr dfdrdydrdd rsrervency nsqsvtpsqq lnaltglpys
       61 sryyelfrkr ialpvweyre kffeylsthq ilvlvgetgs gkttqipqwc vellrqkggr
      121 rgvactqprr vaamsvaarv aeemdvaigq evgysirfed csspktllky mtdgmllrea
      181 msdplleayg vvlldeaher tlatdilmgv lkqvvtqrpd lkivvmsatl dagkfqnyfd
      241 naplmnvpgr thpveifytp eperdyleaa irtviqihmc eeiegdillf ltgqeeieea
      301 ckrlkreidn lgpevgemkc iplysslppn lqqrifeppp pakangaigr kvvvstniae
      361 tsltidgvvf vidpgfakqk vynprirves llvspiskas sqqragragr trpgkcfrly
      421 tekayktemq dqtypeilrs nlgsvvlqlk klgiddlvhf dfmdppapet lmralellny
      481 lqalddngel telgsimaef pldpqlakml itsceyncsn ealsitamls vpqcfvrpne
      541 akkaadeskm rfahidgdhl tllnvyhafk qnhedtqwcy dnfinyrsmk sadnvrqqls
      601 rimdrfnlrr tsteftskdy yinirktlvs gffmqvahle rtghyltikd nqvvqlhpst
      661 cldhkpewvv ynefvlttkn yirtvtdikp ewlikiapny ydmsnfpqce akrqlemlia
      721 kmetrqyqeg f
//
LOCUS       XP_077503103             685 aa            linear   INV 25-NOV-2025
DEFINITION  heat shock protein 70 cognate 5 [Amblyomma americanum].
ACCESSION   XP_077503103
VERSION     XP_077503103.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646977.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..685
                     /product="heat shock protein 70 cognate 5"
                     /calculated_mol_wt=74383
     Region          54..658
                     /region_name="dnaK"
                     /note="molecular chaperone DnaK; Provisional; PRK00290"
                     /db_xref="CDD:234715"
     CDS             1..685
                     /gene="Hsc70-5"
                     /coded_by="XM_077646977.1:117..2174"
                     /db_xref="GeneID:144113711"
ORIGIN      
        1 mlnaarvssr avlssnsvte sllrrsfvtl tskdhllekn gcgqqvrnks dqvkgavigi
       61 dlgttnscva vmegktpkvi ensegsrttp svvaftadge rlvgmpakrq avtnasntls
      121 atkrligrkf edpevkkdmq tqsykivras ngdawveahg kmyspsqiga fvlmkmketa
      181 egylghsvkn avitvpayfn dsqrqatkda gqiaglnvlr vineptaaal aygmdktddk
      241 iiavydlggg tfdisvleiq kgvfevkstn gdtflggedf dnalvkffvq efkreqgidv
      301 tkdnmamqrl keaaekakie lsssvqtdin lpyltmdqsg pkhmtlklsr skfeglvadl
      361 irrtvepckk amqdgevkrt digevllvgg mtrmpkvqet vqeifgkqps kavnpdeava
      421 vgaaiqggvl agdvtdvlll dvtplslgie tlggvftkli nrnttiptkk sqvfstaadg
      481 qtqveikvhq geremacdnk llgqfslvgi ppaprgvpqi evtfdidang ivhvsardkg
      541 tgkeqqiviq ssgglskeei enmiknaery aeadkqkket veavnqaegi ihdteskmde
      601 fkdqlpqeec dklkekitkv reilsnkdke sadsikqatn dlqqaslklf emaykkmase
      661 rassessgss dsssqenaek keekn
//
LOCUS       XP_077503104             327 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113712 [Amblyomma americanum].
ACCESSION   XP_077503104
VERSION     XP_077503104.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646978.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..327
                     /product="uncharacterized protein LOC144113712"
                     /calculated_mol_wt=36896
     Region          <110..150
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     Region          159..>256
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     CDS             1..327
                     /gene="LOC144113712"
                     /coded_by="XM_077646978.1:1..984"
                     /db_xref="GeneID:144113712"
ORIGIN      
        1 mseetkvssq pflvqppvsf nfdktsewpg wiqefddyrf asglnertqe apvrtllytm
       61 grqarnifkt fnlseedskk ydmvktqfdt hfvatrnlvy esacfyrrrq epgesvdqyv
      121 telhtladrc dygemkermi ldrfvvglrd aklsealqid aaltlksalt karlkeavqk
      181 qqqdlrsvvd reehesltra evdivhrrts nqrrpdhqtc aacgrgphsr sscpargalc
      241 ylcqrrghfa avcswthrtt epeawpsevg icsprgeqpn mncvtfsvgd lavssakarf
      301 vqiaingavv sakvdtgaek qnvktas
//
LOCUS       XP_077503105             325 aa            linear   INV 25-NOV-2025
DEFINITION  protein-L-histidine N-pros-methyltransferase isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503105
VERSION     XP_077503105.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646979.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..325
                     /product="protein-L-histidine N-pros-methyltransferase
                     isoform X1"
                     /calculated_mol_wt=36431
     Region          43..315
                     /region_name="AdoMet_MTases"
                     /note="S-adenosylmethionine-dependent methyltransferases
                     (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes
                     that use S-adenosyl-L-methionine (SAM or AdoMet) as a
                     substrate for methyltransfer, creating the product
                     S-adenosyl-L-homocysteine (AdoHcy); cl17173"
                     /db_xref="CDD:473071"
     Site            order(144..150,166..167,183..185,208)
                     /site_type="other"
                     /note="S-adenosylmethionine binding site [chemical
                     binding]"
                     /db_xref="CDD:100107"
     CDS             1..325
                     /gene="LOC144113713"
                     /coded_by="XM_077646979.1:132..1109"
                     /db_xref="GeneID:144113713"
ORIGIN      
        1 mcneplprtm tnirpilhsp laralydrll rdqdlerldk sqwysintel lrdevlrsqf
       61 vqceldadtq tfldvsteks nwlgtqimha larlffgwfv trttlngflg rgsmfvfstr
      121 qflrllrlda estaaplfss lldvgagdgn vtaaiaplfc qvdvtekspa mrrtlsrrgf
      181 rvldaatlrp agldddgepq lydvvcclnv ldrcdtplal lrmlkarlts pagrlvlavv
      241 lplsqyveag krgtkplevl rvrgstleqq veslyrdvma pagfileswt rlpylcegdl
      301 eqayywlddi vmvlraadah gddps
//
LOCUS       XP_077503106             316 aa            linear   INV 25-NOV-2025
DEFINITION  protein-L-histidine N-pros-methyltransferase isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503106
VERSION     XP_077503106.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646980.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..316
                     /product="protein-L-histidine N-pros-methyltransferase
                     isoform X2"
                     /calculated_mol_wt=35389
     Region          34..306
                     /region_name="AdoMet_MTases"
                     /note="S-adenosylmethionine-dependent methyltransferases
                     (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes
                     that use S-adenosyl-L-methionine (SAM or AdoMet) as a
                     substrate for methyltransfer, creating the product
                     S-adenosyl-L-homocysteine (AdoHcy); cl17173"
                     /db_xref="CDD:473071"
     Site            order(135..141,157..158,174..176,199)
                     /site_type="other"
                     /note="S-adenosylmethionine binding site [chemical
                     binding]"
                     /db_xref="CDD:100107"
     CDS             1..316
                     /gene="LOC144113713"
                     /coded_by="XM_077646980.1:59..1009"
                     /db_xref="GeneID:144113713"
ORIGIN      
        1 mtnirpilhs plaralydrl lrdqdlerld ksqwysinte llrdevlrsq fvqceldadt
       61 qtfldvstek snwlgtqimh alarlffgwf vtrttlngfl grgsmfvfst rqflrllrld
      121 aestaaplfs slldvgagdg nvtaaiaplf cqvdvteksp amrrtlsrrg frvldaatlr
      181 pagldddgep qlydvvccln vldrcdtpla llrmlkarlt spagrlvlav vlplsqyvea
      241 gkrgtkplev lrvrgstleq qveslyrdvm apagfilesw trlpylcegd leqayywldd
      301 ivmvlraada hgddps
//
LOCUS       XP_077503107             316 aa            linear   INV 25-NOV-2025
DEFINITION  protein-L-histidine N-pros-methyltransferase isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503107
VERSION     XP_077503107.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646981.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..316
                     /product="protein-L-histidine N-pros-methyltransferase
                     isoform X2"
                     /calculated_mol_wt=35389
     Region          34..306
                     /region_name="AdoMet_MTases"
                     /note="S-adenosylmethionine-dependent methyltransferases
                     (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes
                     that use S-adenosyl-L-methionine (SAM or AdoMet) as a
                     substrate for methyltransfer, creating the product
                     S-adenosyl-L-homocysteine (AdoHcy); cl17173"
                     /db_xref="CDD:473071"
     Site            order(135..141,157..158,174..176,199)
                     /site_type="other"
                     /note="S-adenosylmethionine binding site [chemical
                     binding]"
                     /db_xref="CDD:100107"
     CDS             1..316
                     /gene="LOC144113713"
                     /coded_by="XM_077646981.1:200..1150"
                     /db_xref="GeneID:144113713"
ORIGIN      
        1 mtnirpilhs plaralydrl lrdqdlerld ksqwysinte llrdevlrsq fvqceldadt
       61 qtfldvstek snwlgtqimh alarlffgwf vtrttlngfl grgsmfvfst rqflrllrld
      121 aestaaplfs slldvgagdg nvtaaiaplf cqvdvteksp amrrtlsrrg frvldaatlr
      181 pagldddgep qlydvvccln vldrcdtpla llrmlkarlt spagrlvlav vlplsqyvea
      241 gkrgtkplev lrvrgstleq qveslyrdvm apagfilesw trlpylcegd leqayywldd
      301 ivmvlraada hgddps
//
LOCUS       XP_077503108            3122 aa            linear   INV 25-NOV-2025
DEFINITION  Zn finger homeodomain 2 [Amblyomma americanum].
ACCESSION   XP_077503108
VERSION     XP_077503108.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646982.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..3122
                     /product="Zn finger homeodomain 2"
                     /calculated_mol_wt=341382
     Region          465..486
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(465,468,481,486)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(470,472,474,476..477,480..481,485,501,503,507..508,
                     511..512,541,556,558,560,562..563,566..567)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          <492..555
                     /region_name="SFP1"
                     /note="Putative transcriptional repressor regulating G2/M
                     transition [Transcription / Cell division and chromosome
                     partitioning]; COG5189"
                     /db_xref="CDD:227516"
     Region          496..542
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          549..573
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          551..568
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1249..1269
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Site            order(1256..1258,1260..1261,1265,1268,1282,1285..1289,
                     1291..1292)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275371"
     Region          1277..1298
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Region          1409..1431
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1453..1469
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1552..1570
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(2127..2131,2133,2150,2156,2169,2171..2172,
                     2175..2176,2178..2180,2182..2183)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238039"
     Region          2127..2182
                     /region_name="HOX"
                     /note="Homeodomain; smart00389"
                     /db_xref="CDD:197696"
     Site            order(2129,2132,2172,2175..2176,2179)
                     /site_type="other"
                     /note="specific DNA base contacts [nucleotide binding]"
                     /db_xref="CDD:238039"
     Region          2386..2442
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     Region          2648..2704
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     Region          2909..2965
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..3122
                     /gene="zfh2"
                     /coded_by="XM_077646982.1:390..9758"
                     /db_xref="GeneID:144113714"
ORIGIN      
        1 meapqrsmdt gqqpavvasm tpedcpasga ergdksadgg sppsqqfked padasdqsss
       61 lsdvetfngk ivynpdgsaf iietatagds dgvsdedcsl dlphqegsiv dgpgvtpppi
      121 ipqvvnafhi srnpalysal ygqaysllqe kkkvpevpim hsyrvfsvrd qknkdekgat
      181 ddsagdekkv srppgwsall nttlplmehp svpikpilmc ficklsfgft ksfvghaige
      241 hgitltdeer hmmnqknisa iiqgvgkdke pllsfleplp aqgaaggpcg sgkfqaknpi
      301 asssssssla paeagvgpll santngpenp saqdarqspq apvltppgss nashrpdetl
      361 apeledlani ekiakaaaaa aavvenppvv tasaektmpv alpagsdptp rtsppsaerp
      421 msagsssspg vspkllpqpp flnacaspvm acpqhpdgkt ngvecpkcdt vlgssrslgg
      481 hmtmmhsrns cktlkcpkcn whykyqetle ihmkekhpen elnciyclts qphprlarge
      541 tytcgykpyr cevcnysttt kgnlsihmqs dkhinniqel qngnlqatpe qllqqqqppq
      601 qqqppalpea vkkpgpaakp katwrcdvcn yetnvarnlr ihmtsekhth nmmvlqqnvk
      661 hmqqlsalqq aqaldplfqf hpglllpcdg qlqpeaalad maynhallmm asqqhqqqqa
      721 laaaaagspl asptmdmehp dptlrlelst ddtsqlfqcc vcglfsvdsv ealgahfqqd
      781 rtrqredevl mavagsyvck lcsyktnlra nfqlhcktdk hlqrlqhvnh ikeggprndw
      841 klkyvnvsnp vqvrcnacdy ytnsvhklql haasarhdlv arlflflvaa etnlrgdvar
      901 hyqctacgfh srtrsgllgh ahslrhlqqt rakddgsesw kdtflvkeyv gnehvsfdvd
      961 venkgteame vdlpppvlpv edresanksg gpsspgsmlk qallenaaka eaaasshrse
     1021 eektserhtc klcnftaksq tllhahvssq hssqhqaqrq pksvqcplcq eqfkalekie
     1081 shlvethnvt tegmqrllal vdtsvwatta aaavkrgass atkqnalpaa nddsdsadad
     1141 cqasdsenrd kmilddclsi edltcgacgk nfsvleelfs hqtetghlel kqtprgpgyl
     1201 cwkkacnqyf mtpqavqvhf keihsrrsqq qqhqhqlavs erhvykyrcn qcslafktle
     1261 klqlhsqyhv iraatqcvlc grsfrsvaal qkhvetshid mtpeeleqyk tslvnnpllt
     1321 sgggavldpq ttellkkesn reedaavdee mdtddgaqvn endggvepmd asvpglegts
     1381 lkeqffedyi nsqaiaedsy ndpgrkykch rckvaftrqs yltshnktll hrkgeklsyp
     1441 mekyldpnrp ykcdickesf tqknillvhy nsvshlhklk lslkdgsspa pslpssavna
     1501 ipltvapssn csnsvtshta iappqsptls qvmahsptnn nnndsekkpf kcnickvayt
     1561 qgstldihmr svlhqtrask lhelamtgqv dlsspliekp esglavqses pqptqqksls
     1621 dmltpskpsq qslelsqkqa eassqnlshg hsapstpvpa ststtptsqd hsessclhhv
     1681 itstvshigp qpvllssqma spqgmvtcnr csailmsqea lvqhqqlycv aasaaqstsp
     1741 lvvtqkhsih shhhqhhtpq qmmpenqlsn pqlqaaatat qqllrlrspy prskppmykh
     1801 llegfgfdvv mqfnefnqkr mkrdaekreq ilqqqqqqqp keeldkpmsp kveavqvkke
     1861 teeiepeken tmpeinrsvc nicqkefssi wvlkahreev hhdivpldfv skladdfrte
     1921 ydkknasqvp aeegmldlas sqaaggqadg aspgasvgvs tvgvggnggs sgagggsggs
     1981 gntnssssnt naaghspqlp tpasatppvs lsvapqasqq qpvseastvt anqmaaqlqf
     2041 nqllmsmglg mglpmgmnmp faaamnmhpp lipvvmpphm dplitsafnh pmmpgamdps
     2101 ffaaqqkllq qqhqqlaqaq qqaqqqkrar trisdeqlri lrayfdinns pteeqlmems
     2161 eksglplkvi khwfrntlfk erqrnkdspy nfnnppstyl nleeyektge akviplsekn
     2221 nnaedgsisa vkpvppsaas keapsasttt tttttppsev pvkkdsiree arieaastdt
     2281 qdsvassntt qngegalltp kveaptlqss qhnsgggsss sgggggksef lssltsrlsv
     2341 dppldmaspv hhgfllgtpp pllerssstt ptpmtstpsa gssgkranrt rftdyqikvl
     2401 qeffetnayp kdddleylsk llnlsprviv vwfqnarqka rkvyenqpps apeddgsgrf
     2461 qrtpglnyqc kkclqvfqry yelikhqkts cfkdenplaa qlkaaasgsg tgtgssgggs
     2521 gddrslvgas npssprtpep pckspqngsy rcdkcslvfp rfdlwrehqi vhimnpnlfp
     2581 nyppnssfgi lqyegqqqqq qialpspqqq qqqqqqqpwk rkleeeqplq lqqqqdskdl
     2641 tgepprdkrl rttilpeqld ylyqkyqmes npsrkmleni ardvglkkrv vqvwfqntra
     2701 rerkgqfrah qqvihkrcpf cralfkarsa leshlatrha dlyskgelni dsfpdgdads
     2761 npgtptssas eeskaispsa gsdlvhstmk kyyedslkky ldemtgaptd lsvkpkanka
     2821 disggeapld lskplrldsd rsgdhlsvse ksmdlrigvs ddarsethse stdnmdgdds
     2881 yfesnptspl glhgaggqqp srpvsgsgkr frtqmttvql kvmksifady ktpsmaecem
     2941 lgreiglpkr vvqvwfqnar akekksklaf aktfgqeiep pkppeecslc svkynhqfsn
     3001 tsmqdhlfsk rhidalrnhi dnikkmtddg aperaadadk apslvqqlqm lgqglppsfa
     3061 lpspkedakk gdepkkddap ppppdslapy lyaaglpgyy paaagvpgaf ihptlfsgrn
     3121 kl
//
LOCUS       XP_077503109            3122 aa            linear   INV 25-NOV-2025
DEFINITION  Zn finger homeodomain 2 [Amblyomma americanum].
ACCESSION   XP_077503109
VERSION     XP_077503109.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646983.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..3122
                     /product="Zn finger homeodomain 2"
                     /calculated_mol_wt=341382
     Region          465..486
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(465,468,481,486)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(470,472,474,476..477,480..481,485,501,503,507..508,
                     511..512,541,556,558,560,562..563,566..567)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          <492..555
                     /region_name="SFP1"
                     /note="Putative transcriptional repressor regulating G2/M
                     transition [Transcription / Cell division and chromosome
                     partitioning]; COG5189"
                     /db_xref="CDD:227516"
     Region          496..542
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          549..573
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          551..568
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1249..1269
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Site            order(1256..1258,1260..1261,1265,1268,1282,1285..1289,
                     1291..1292)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275371"
     Region          1277..1298
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Region          1409..1431
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1453..1469
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1552..1570
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(2127..2131,2133,2150,2156,2169,2171..2172,
                     2175..2176,2178..2180,2182..2183)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238039"
     Region          2127..2182
                     /region_name="HOX"
                     /note="Homeodomain; smart00389"
                     /db_xref="CDD:197696"
     Site            order(2129,2132,2172,2175..2176,2179)
                     /site_type="other"
                     /note="specific DNA base contacts [nucleotide binding]"
                     /db_xref="CDD:238039"
     Region          2386..2442
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     Region          2648..2704
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     Region          2909..2965
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..3122
                     /gene="zfh2"
                     /coded_by="XM_077646983.1:698..10066"
                     /db_xref="GeneID:144113714"
ORIGIN      
        1 meapqrsmdt gqqpavvasm tpedcpasga ergdksadgg sppsqqfked padasdqsss
       61 lsdvetfngk ivynpdgsaf iietatagds dgvsdedcsl dlphqegsiv dgpgvtpppi
      121 ipqvvnafhi srnpalysal ygqaysllqe kkkvpevpim hsyrvfsvrd qknkdekgat
      181 ddsagdekkv srppgwsall nttlplmehp svpikpilmc ficklsfgft ksfvghaige
      241 hgitltdeer hmmnqknisa iiqgvgkdke pllsfleplp aqgaaggpcg sgkfqaknpi
      301 asssssssla paeagvgpll santngpenp saqdarqspq apvltppgss nashrpdetl
      361 apeledlani ekiakaaaaa aavvenppvv tasaektmpv alpagsdptp rtsppsaerp
      421 msagsssspg vspkllpqpp flnacaspvm acpqhpdgkt ngvecpkcdt vlgssrslgg
      481 hmtmmhsrns cktlkcpkcn whykyqetle ihmkekhpen elnciyclts qphprlarge
      541 tytcgykpyr cevcnysttt kgnlsihmqs dkhinniqel qngnlqatpe qllqqqqppq
      601 qqqppalpea vkkpgpaakp katwrcdvcn yetnvarnlr ihmtsekhth nmmvlqqnvk
      661 hmqqlsalqq aqaldplfqf hpglllpcdg qlqpeaalad maynhallmm asqqhqqqqa
      721 laaaaagspl asptmdmehp dptlrlelst ddtsqlfqcc vcglfsvdsv ealgahfqqd
      781 rtrqredevl mavagsyvck lcsyktnlra nfqlhcktdk hlqrlqhvnh ikeggprndw
      841 klkyvnvsnp vqvrcnacdy ytnsvhklql haasarhdlv arlflflvaa etnlrgdvar
      901 hyqctacgfh srtrsgllgh ahslrhlqqt rakddgsesw kdtflvkeyv gnehvsfdvd
      961 venkgteame vdlpppvlpv edresanksg gpsspgsmlk qallenaaka eaaasshrse
     1021 eektserhtc klcnftaksq tllhahvssq hssqhqaqrq pksvqcplcq eqfkalekie
     1081 shlvethnvt tegmqrllal vdtsvwatta aaavkrgass atkqnalpaa nddsdsadad
     1141 cqasdsenrd kmilddclsi edltcgacgk nfsvleelfs hqtetghlel kqtprgpgyl
     1201 cwkkacnqyf mtpqavqvhf keihsrrsqq qqhqhqlavs erhvykyrcn qcslafktle
     1261 klqlhsqyhv iraatqcvlc grsfrsvaal qkhvetshid mtpeeleqyk tslvnnpllt
     1321 sgggavldpq ttellkkesn reedaavdee mdtddgaqvn endggvepmd asvpglegts
     1381 lkeqffedyi nsqaiaedsy ndpgrkykch rckvaftrqs yltshnktll hrkgeklsyp
     1441 mekyldpnrp ykcdickesf tqknillvhy nsvshlhklk lslkdgsspa pslpssavna
     1501 ipltvapssn csnsvtshta iappqsptls qvmahsptnn nnndsekkpf kcnickvayt
     1561 qgstldihmr svlhqtrask lhelamtgqv dlsspliekp esglavqses pqptqqksls
     1621 dmltpskpsq qslelsqkqa eassqnlshg hsapstpvpa ststtptsqd hsessclhhv
     1681 itstvshigp qpvllssqma spqgmvtcnr csailmsqea lvqhqqlycv aasaaqstsp
     1741 lvvtqkhsih shhhqhhtpq qmmpenqlsn pqlqaaatat qqllrlrspy prskppmykh
     1801 llegfgfdvv mqfnefnqkr mkrdaekreq ilqqqqqqqp keeldkpmsp kveavqvkke
     1861 teeiepeken tmpeinrsvc nicqkefssi wvlkahreev hhdivpldfv skladdfrte
     1921 ydkknasqvp aeegmldlas sqaaggqadg aspgasvgvs tvgvggnggs sgagggsggs
     1981 gntnssssnt naaghspqlp tpasatppvs lsvapqasqq qpvseastvt anqmaaqlqf
     2041 nqllmsmglg mglpmgmnmp faaamnmhpp lipvvmpphm dplitsafnh pmmpgamdps
     2101 ffaaqqkllq qqhqqlaqaq qqaqqqkrar trisdeqlri lrayfdinns pteeqlmems
     2161 eksglplkvi khwfrntlfk erqrnkdspy nfnnppstyl nleeyektge akviplsekn
     2221 nnaedgsisa vkpvppsaas keapsasttt tttttppsev pvkkdsiree arieaastdt
     2281 qdsvassntt qngegalltp kveaptlqss qhnsgggsss sgggggksef lssltsrlsv
     2341 dppldmaspv hhgfllgtpp pllerssstt ptpmtstpsa gssgkranrt rftdyqikvl
     2401 qeffetnayp kdddleylsk llnlsprviv vwfqnarqka rkvyenqpps apeddgsgrf
     2461 qrtpglnyqc kkclqvfqry yelikhqkts cfkdenplaa qlkaaasgsg tgtgssgggs
     2521 gddrslvgas npssprtpep pckspqngsy rcdkcslvfp rfdlwrehqi vhimnpnlfp
     2581 nyppnssfgi lqyegqqqqq qialpspqqq qqqqqqqpwk rkleeeqplq lqqqqdskdl
     2641 tgepprdkrl rttilpeqld ylyqkyqmes npsrkmleni ardvglkkrv vqvwfqntra
     2701 rerkgqfrah qqvihkrcpf cralfkarsa leshlatrha dlyskgelni dsfpdgdads
     2761 npgtptssas eeskaispsa gsdlvhstmk kyyedslkky ldemtgaptd lsvkpkanka
     2821 disggeapld lskplrldsd rsgdhlsvse ksmdlrigvs ddarsethse stdnmdgdds
     2881 yfesnptspl glhgaggqqp srpvsgsgkr frtqmttvql kvmksifady ktpsmaecem
     2941 lgreiglpkr vvqvwfqnar akekksklaf aktfgqeiep pkppeecslc svkynhqfsn
     3001 tsmqdhlfsk rhidalrnhi dnikkmtddg aperaadadk apslvqqlqm lgqglppsfa
     3061 lpspkedakk gdepkkddap ppppdslapy lyaaglpgyy paaagvpgaf ihptlfsgrn
     3121 kl
//
LOCUS       XP_077503110            3122 aa            linear   INV 25-NOV-2025
DEFINITION  Zn finger homeodomain 2 [Amblyomma americanum].
ACCESSION   XP_077503110
VERSION     XP_077503110.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646984.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..3122
                     /product="Zn finger homeodomain 2"
                     /calculated_mol_wt=341382
     Region          465..486
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(465,468,481,486)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(470,472,474,476..477,480..481,485,501,503,507..508,
                     511..512,541,556,558,560,562..563,566..567)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          <492..555
                     /region_name="SFP1"
                     /note="Putative transcriptional repressor regulating G2/M
                     transition [Transcription / Cell division and chromosome
                     partitioning]; COG5189"
                     /db_xref="CDD:227516"
     Region          496..542
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          549..573
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          551..568
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1249..1269
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Site            order(1256..1258,1260..1261,1265,1268,1282,1285..1289,
                     1291..1292)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275371"
     Region          1277..1298
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Region          1409..1431
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1453..1469
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1552..1570
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(2127..2131,2133,2150,2156,2169,2171..2172,
                     2175..2176,2178..2180,2182..2183)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238039"
     Region          2127..2182
                     /region_name="HOX"
                     /note="Homeodomain; smart00389"
                     /db_xref="CDD:197696"
     Site            order(2129,2132,2172,2175..2176,2179)
                     /site_type="other"
                     /note="specific DNA base contacts [nucleotide binding]"
                     /db_xref="CDD:238039"
     Region          2386..2442
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     Region          2648..2704
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     Region          2909..2965
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..3122
                     /gene="zfh2"
                     /coded_by="XM_077646984.1:293..9661"
                     /db_xref="GeneID:144113714"
ORIGIN      
        1 meapqrsmdt gqqpavvasm tpedcpasga ergdksadgg sppsqqfked padasdqsss
       61 lsdvetfngk ivynpdgsaf iietatagds dgvsdedcsl dlphqegsiv dgpgvtpppi
      121 ipqvvnafhi srnpalysal ygqaysllqe kkkvpevpim hsyrvfsvrd qknkdekgat
      181 ddsagdekkv srppgwsall nttlplmehp svpikpilmc ficklsfgft ksfvghaige
      241 hgitltdeer hmmnqknisa iiqgvgkdke pllsfleplp aqgaaggpcg sgkfqaknpi
      301 asssssssla paeagvgpll santngpenp saqdarqspq apvltppgss nashrpdetl
      361 apeledlani ekiakaaaaa aavvenppvv tasaektmpv alpagsdptp rtsppsaerp
      421 msagsssspg vspkllpqpp flnacaspvm acpqhpdgkt ngvecpkcdt vlgssrslgg
      481 hmtmmhsrns cktlkcpkcn whykyqetle ihmkekhpen elnciyclts qphprlarge
      541 tytcgykpyr cevcnysttt kgnlsihmqs dkhinniqel qngnlqatpe qllqqqqppq
      601 qqqppalpea vkkpgpaakp katwrcdvcn yetnvarnlr ihmtsekhth nmmvlqqnvk
      661 hmqqlsalqq aqaldplfqf hpglllpcdg qlqpeaalad maynhallmm asqqhqqqqa
      721 laaaaagspl asptmdmehp dptlrlelst ddtsqlfqcc vcglfsvdsv ealgahfqqd
      781 rtrqredevl mavagsyvck lcsyktnlra nfqlhcktdk hlqrlqhvnh ikeggprndw
      841 klkyvnvsnp vqvrcnacdy ytnsvhklql haasarhdlv arlflflvaa etnlrgdvar
      901 hyqctacgfh srtrsgllgh ahslrhlqqt rakddgsesw kdtflvkeyv gnehvsfdvd
      961 venkgteame vdlpppvlpv edresanksg gpsspgsmlk qallenaaka eaaasshrse
     1021 eektserhtc klcnftaksq tllhahvssq hssqhqaqrq pksvqcplcq eqfkalekie
     1081 shlvethnvt tegmqrllal vdtsvwatta aaavkrgass atkqnalpaa nddsdsadad
     1141 cqasdsenrd kmilddclsi edltcgacgk nfsvleelfs hqtetghlel kqtprgpgyl
     1201 cwkkacnqyf mtpqavqvhf keihsrrsqq qqhqhqlavs erhvykyrcn qcslafktle
     1261 klqlhsqyhv iraatqcvlc grsfrsvaal qkhvetshid mtpeeleqyk tslvnnpllt
     1321 sgggavldpq ttellkkesn reedaavdee mdtddgaqvn endggvepmd asvpglegts
     1381 lkeqffedyi nsqaiaedsy ndpgrkykch rckvaftrqs yltshnktll hrkgeklsyp
     1441 mekyldpnrp ykcdickesf tqknillvhy nsvshlhklk lslkdgsspa pslpssavna
     1501 ipltvapssn csnsvtshta iappqsptls qvmahsptnn nnndsekkpf kcnickvayt
     1561 qgstldihmr svlhqtrask lhelamtgqv dlsspliekp esglavqses pqptqqksls
     1621 dmltpskpsq qslelsqkqa eassqnlshg hsapstpvpa ststtptsqd hsessclhhv
     1681 itstvshigp qpvllssqma spqgmvtcnr csailmsqea lvqhqqlycv aasaaqstsp
     1741 lvvtqkhsih shhhqhhtpq qmmpenqlsn pqlqaaatat qqllrlrspy prskppmykh
     1801 llegfgfdvv mqfnefnqkr mkrdaekreq ilqqqqqqqp keeldkpmsp kveavqvkke
     1861 teeiepeken tmpeinrsvc nicqkefssi wvlkahreev hhdivpldfv skladdfrte
     1921 ydkknasqvp aeegmldlas sqaaggqadg aspgasvgvs tvgvggnggs sgagggsggs
     1981 gntnssssnt naaghspqlp tpasatppvs lsvapqasqq qpvseastvt anqmaaqlqf
     2041 nqllmsmglg mglpmgmnmp faaamnmhpp lipvvmpphm dplitsafnh pmmpgamdps
     2101 ffaaqqkllq qqhqqlaqaq qqaqqqkrar trisdeqlri lrayfdinns pteeqlmems
     2161 eksglplkvi khwfrntlfk erqrnkdspy nfnnppstyl nleeyektge akviplsekn
     2221 nnaedgsisa vkpvppsaas keapsasttt tttttppsev pvkkdsiree arieaastdt
     2281 qdsvassntt qngegalltp kveaptlqss qhnsgggsss sgggggksef lssltsrlsv
     2341 dppldmaspv hhgfllgtpp pllerssstt ptpmtstpsa gssgkranrt rftdyqikvl
     2401 qeffetnayp kdddleylsk llnlsprviv vwfqnarqka rkvyenqpps apeddgsgrf
     2461 qrtpglnyqc kkclqvfqry yelikhqkts cfkdenplaa qlkaaasgsg tgtgssgggs
     2521 gddrslvgas npssprtpep pckspqngsy rcdkcslvfp rfdlwrehqi vhimnpnlfp
     2581 nyppnssfgi lqyegqqqqq qialpspqqq qqqqqqqpwk rkleeeqplq lqqqqdskdl
     2641 tgepprdkrl rttilpeqld ylyqkyqmes npsrkmleni ardvglkkrv vqvwfqntra
     2701 rerkgqfrah qqvihkrcpf cralfkarsa leshlatrha dlyskgelni dsfpdgdads
     2761 npgtptssas eeskaispsa gsdlvhstmk kyyedslkky ldemtgaptd lsvkpkanka
     2821 disggeapld lskplrldsd rsgdhlsvse ksmdlrigvs ddarsethse stdnmdgdds
     2881 yfesnptspl glhgaggqqp srpvsgsgkr frtqmttvql kvmksifady ktpsmaecem
     2941 lgreiglpkr vvqvwfqnar akekksklaf aktfgqeiep pkppeecslc svkynhqfsn
     3001 tsmqdhlfsk rhidalrnhi dnikkmtddg aperaadadk apslvqqlqm lgqglppsfa
     3061 lpspkedakk gdepkkddap ppppdslapy lyaaglpgyy paaagvpgaf ihptlfsgrn
     3121 kl
//
LOCUS       XP_077503111            3122 aa            linear   INV 25-NOV-2025
DEFINITION  Zn finger homeodomain 2 [Amblyomma americanum].
ACCESSION   XP_077503111
VERSION     XP_077503111.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646985.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..3122
                     /product="Zn finger homeodomain 2"
                     /calculated_mol_wt=341382
     Region          465..486
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(465,468,481,486)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(470,472,474,476..477,480..481,485,501,503,507..508,
                     511..512,541,556,558,560,562..563,566..567)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          <492..555
                     /region_name="SFP1"
                     /note="Putative transcriptional repressor regulating G2/M
                     transition [Transcription / Cell division and chromosome
                     partitioning]; COG5189"
                     /db_xref="CDD:227516"
     Region          496..542
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          549..573
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          551..568
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1249..1269
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Site            order(1256..1258,1260..1261,1265,1268,1282,1285..1289,
                     1291..1292)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275371"
     Region          1277..1298
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Region          1409..1431
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1453..1469
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1552..1570
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(2127..2131,2133,2150,2156,2169,2171..2172,
                     2175..2176,2178..2180,2182..2183)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238039"
     Region          2127..2182
                     /region_name="HOX"
                     /note="Homeodomain; smart00389"
                     /db_xref="CDD:197696"
     Site            order(2129,2132,2172,2175..2176,2179)
                     /site_type="other"
                     /note="specific DNA base contacts [nucleotide binding]"
                     /db_xref="CDD:238039"
     Region          2386..2442
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     Region          2648..2704
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     Region          2909..2965
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..3122
                     /gene="zfh2"
                     /coded_by="XM_077646985.1:647..10015"
                     /db_xref="GeneID:144113714"
ORIGIN      
        1 meapqrsmdt gqqpavvasm tpedcpasga ergdksadgg sppsqqfked padasdqsss
       61 lsdvetfngk ivynpdgsaf iietatagds dgvsdedcsl dlphqegsiv dgpgvtpppi
      121 ipqvvnafhi srnpalysal ygqaysllqe kkkvpevpim hsyrvfsvrd qknkdekgat
      181 ddsagdekkv srppgwsall nttlplmehp svpikpilmc ficklsfgft ksfvghaige
      241 hgitltdeer hmmnqknisa iiqgvgkdke pllsfleplp aqgaaggpcg sgkfqaknpi
      301 asssssssla paeagvgpll santngpenp saqdarqspq apvltppgss nashrpdetl
      361 apeledlani ekiakaaaaa aavvenppvv tasaektmpv alpagsdptp rtsppsaerp
      421 msagsssspg vspkllpqpp flnacaspvm acpqhpdgkt ngvecpkcdt vlgssrslgg
      481 hmtmmhsrns cktlkcpkcn whykyqetle ihmkekhpen elnciyclts qphprlarge
      541 tytcgykpyr cevcnysttt kgnlsihmqs dkhinniqel qngnlqatpe qllqqqqppq
      601 qqqppalpea vkkpgpaakp katwrcdvcn yetnvarnlr ihmtsekhth nmmvlqqnvk
      661 hmqqlsalqq aqaldplfqf hpglllpcdg qlqpeaalad maynhallmm asqqhqqqqa
      721 laaaaagspl asptmdmehp dptlrlelst ddtsqlfqcc vcglfsvdsv ealgahfqqd
      781 rtrqredevl mavagsyvck lcsyktnlra nfqlhcktdk hlqrlqhvnh ikeggprndw
      841 klkyvnvsnp vqvrcnacdy ytnsvhklql haasarhdlv arlflflvaa etnlrgdvar
      901 hyqctacgfh srtrsgllgh ahslrhlqqt rakddgsesw kdtflvkeyv gnehvsfdvd
      961 venkgteame vdlpppvlpv edresanksg gpsspgsmlk qallenaaka eaaasshrse
     1021 eektserhtc klcnftaksq tllhahvssq hssqhqaqrq pksvqcplcq eqfkalekie
     1081 shlvethnvt tegmqrllal vdtsvwatta aaavkrgass atkqnalpaa nddsdsadad
     1141 cqasdsenrd kmilddclsi edltcgacgk nfsvleelfs hqtetghlel kqtprgpgyl
     1201 cwkkacnqyf mtpqavqvhf keihsrrsqq qqhqhqlavs erhvykyrcn qcslafktle
     1261 klqlhsqyhv iraatqcvlc grsfrsvaal qkhvetshid mtpeeleqyk tslvnnpllt
     1321 sgggavldpq ttellkkesn reedaavdee mdtddgaqvn endggvepmd asvpglegts
     1381 lkeqffedyi nsqaiaedsy ndpgrkykch rckvaftrqs yltshnktll hrkgeklsyp
     1441 mekyldpnrp ykcdickesf tqknillvhy nsvshlhklk lslkdgsspa pslpssavna
     1501 ipltvapssn csnsvtshta iappqsptls qvmahsptnn nnndsekkpf kcnickvayt
     1561 qgstldihmr svlhqtrask lhelamtgqv dlsspliekp esglavqses pqptqqksls
     1621 dmltpskpsq qslelsqkqa eassqnlshg hsapstpvpa ststtptsqd hsessclhhv
     1681 itstvshigp qpvllssqma spqgmvtcnr csailmsqea lvqhqqlycv aasaaqstsp
     1741 lvvtqkhsih shhhqhhtpq qmmpenqlsn pqlqaaatat qqllrlrspy prskppmykh
     1801 llegfgfdvv mqfnefnqkr mkrdaekreq ilqqqqqqqp keeldkpmsp kveavqvkke
     1861 teeiepeken tmpeinrsvc nicqkefssi wvlkahreev hhdivpldfv skladdfrte
     1921 ydkknasqvp aeegmldlas sqaaggqadg aspgasvgvs tvgvggnggs sgagggsggs
     1981 gntnssssnt naaghspqlp tpasatppvs lsvapqasqq qpvseastvt anqmaaqlqf
     2041 nqllmsmglg mglpmgmnmp faaamnmhpp lipvvmpphm dplitsafnh pmmpgamdps
     2101 ffaaqqkllq qqhqqlaqaq qqaqqqkrar trisdeqlri lrayfdinns pteeqlmems
     2161 eksglplkvi khwfrntlfk erqrnkdspy nfnnppstyl nleeyektge akviplsekn
     2221 nnaedgsisa vkpvppsaas keapsasttt tttttppsev pvkkdsiree arieaastdt
     2281 qdsvassntt qngegalltp kveaptlqss qhnsgggsss sgggggksef lssltsrlsv
     2341 dppldmaspv hhgfllgtpp pllerssstt ptpmtstpsa gssgkranrt rftdyqikvl
     2401 qeffetnayp kdddleylsk llnlsprviv vwfqnarqka rkvyenqpps apeddgsgrf
     2461 qrtpglnyqc kkclqvfqry yelikhqkts cfkdenplaa qlkaaasgsg tgtgssgggs
     2521 gddrslvgas npssprtpep pckspqngsy rcdkcslvfp rfdlwrehqi vhimnpnlfp
     2581 nyppnssfgi lqyegqqqqq qialpspqqq qqqqqqqpwk rkleeeqplq lqqqqdskdl
     2641 tgepprdkrl rttilpeqld ylyqkyqmes npsrkmleni ardvglkkrv vqvwfqntra
     2701 rerkgqfrah qqvihkrcpf cralfkarsa leshlatrha dlyskgelni dsfpdgdads
     2761 npgtptssas eeskaispsa gsdlvhstmk kyyedslkky ldemtgaptd lsvkpkanka
     2821 disggeapld lskplrldsd rsgdhlsvse ksmdlrigvs ddarsethse stdnmdgdds
     2881 yfesnptspl glhgaggqqp srpvsgsgkr frtqmttvql kvmksifady ktpsmaecem
     2941 lgreiglpkr vvqvwfqnar akekksklaf aktfgqeiep pkppeecslc svkynhqfsn
     3001 tsmqdhlfsk rhidalrnhi dnikkmtddg aperaadadk apslvqqlqm lgqglppsfa
     3061 lpspkedakk gdepkkddap ppppdslapy lyaaglpgyy paaagvpgaf ihptlfsgrn
     3121 kl
//
LOCUS       XP_077503112            3122 aa            linear   INV 25-NOV-2025
DEFINITION  Zn finger homeodomain 2 [Amblyomma americanum].
ACCESSION   XP_077503112
VERSION     XP_077503112.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646986.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..3122
                     /product="Zn finger homeodomain 2"
                     /calculated_mol_wt=341382
     Region          465..486
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(465,468,481,486)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(470,472,474,476..477,480..481,485,501,503,507..508,
                     511..512,541,556,558,560,562..563,566..567)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          <492..555
                     /region_name="SFP1"
                     /note="Putative transcriptional repressor regulating G2/M
                     transition [Transcription / Cell division and chromosome
                     partitioning]; COG5189"
                     /db_xref="CDD:227516"
     Region          496..542
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          549..573
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          551..568
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1249..1269
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Site            order(1256..1258,1260..1261,1265,1268,1282,1285..1289,
                     1291..1292)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275371"
     Region          1277..1298
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Region          1409..1431
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1453..1469
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1552..1570
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(2127..2131,2133,2150,2156,2169,2171..2172,
                     2175..2176,2178..2180,2182..2183)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238039"
     Region          2127..2182
                     /region_name="HOX"
                     /note="Homeodomain; smart00389"
                     /db_xref="CDD:197696"
     Site            order(2129,2132,2172,2175..2176,2179)
                     /site_type="other"
                     /note="specific DNA base contacts [nucleotide binding]"
                     /db_xref="CDD:238039"
     Region          2386..2442
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     Region          2648..2704
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     Region          2909..2965
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..3122
                     /gene="zfh2"
                     /coded_by="XM_077646986.1:357..9725"
                     /db_xref="GeneID:144113714"
ORIGIN      
        1 meapqrsmdt gqqpavvasm tpedcpasga ergdksadgg sppsqqfked padasdqsss
       61 lsdvetfngk ivynpdgsaf iietatagds dgvsdedcsl dlphqegsiv dgpgvtpppi
      121 ipqvvnafhi srnpalysal ygqaysllqe kkkvpevpim hsyrvfsvrd qknkdekgat
      181 ddsagdekkv srppgwsall nttlplmehp svpikpilmc ficklsfgft ksfvghaige
      241 hgitltdeer hmmnqknisa iiqgvgkdke pllsfleplp aqgaaggpcg sgkfqaknpi
      301 asssssssla paeagvgpll santngpenp saqdarqspq apvltppgss nashrpdetl
      361 apeledlani ekiakaaaaa aavvenppvv tasaektmpv alpagsdptp rtsppsaerp
      421 msagsssspg vspkllpqpp flnacaspvm acpqhpdgkt ngvecpkcdt vlgssrslgg
      481 hmtmmhsrns cktlkcpkcn whykyqetle ihmkekhpen elnciyclts qphprlarge
      541 tytcgykpyr cevcnysttt kgnlsihmqs dkhinniqel qngnlqatpe qllqqqqppq
      601 qqqppalpea vkkpgpaakp katwrcdvcn yetnvarnlr ihmtsekhth nmmvlqqnvk
      661 hmqqlsalqq aqaldplfqf hpglllpcdg qlqpeaalad maynhallmm asqqhqqqqa
      721 laaaaagspl asptmdmehp dptlrlelst ddtsqlfqcc vcglfsvdsv ealgahfqqd
      781 rtrqredevl mavagsyvck lcsyktnlra nfqlhcktdk hlqrlqhvnh ikeggprndw
      841 klkyvnvsnp vqvrcnacdy ytnsvhklql haasarhdlv arlflflvaa etnlrgdvar
      901 hyqctacgfh srtrsgllgh ahslrhlqqt rakddgsesw kdtflvkeyv gnehvsfdvd
      961 venkgteame vdlpppvlpv edresanksg gpsspgsmlk qallenaaka eaaasshrse
     1021 eektserhtc klcnftaksq tllhahvssq hssqhqaqrq pksvqcplcq eqfkalekie
     1081 shlvethnvt tegmqrllal vdtsvwatta aaavkrgass atkqnalpaa nddsdsadad
     1141 cqasdsenrd kmilddclsi edltcgacgk nfsvleelfs hqtetghlel kqtprgpgyl
     1201 cwkkacnqyf mtpqavqvhf keihsrrsqq qqhqhqlavs erhvykyrcn qcslafktle
     1261 klqlhsqyhv iraatqcvlc grsfrsvaal qkhvetshid mtpeeleqyk tslvnnpllt
     1321 sgggavldpq ttellkkesn reedaavdee mdtddgaqvn endggvepmd asvpglegts
     1381 lkeqffedyi nsqaiaedsy ndpgrkykch rckvaftrqs yltshnktll hrkgeklsyp
     1441 mekyldpnrp ykcdickesf tqknillvhy nsvshlhklk lslkdgsspa pslpssavna
     1501 ipltvapssn csnsvtshta iappqsptls qvmahsptnn nnndsekkpf kcnickvayt
     1561 qgstldihmr svlhqtrask lhelamtgqv dlsspliekp esglavqses pqptqqksls
     1621 dmltpskpsq qslelsqkqa eassqnlshg hsapstpvpa ststtptsqd hsessclhhv
     1681 itstvshigp qpvllssqma spqgmvtcnr csailmsqea lvqhqqlycv aasaaqstsp
     1741 lvvtqkhsih shhhqhhtpq qmmpenqlsn pqlqaaatat qqllrlrspy prskppmykh
     1801 llegfgfdvv mqfnefnqkr mkrdaekreq ilqqqqqqqp keeldkpmsp kveavqvkke
     1861 teeiepeken tmpeinrsvc nicqkefssi wvlkahreev hhdivpldfv skladdfrte
     1921 ydkknasqvp aeegmldlas sqaaggqadg aspgasvgvs tvgvggnggs sgagggsggs
     1981 gntnssssnt naaghspqlp tpasatppvs lsvapqasqq qpvseastvt anqmaaqlqf
     2041 nqllmsmglg mglpmgmnmp faaamnmhpp lipvvmpphm dplitsafnh pmmpgamdps
     2101 ffaaqqkllq qqhqqlaqaq qqaqqqkrar trisdeqlri lrayfdinns pteeqlmems
     2161 eksglplkvi khwfrntlfk erqrnkdspy nfnnppstyl nleeyektge akviplsekn
     2221 nnaedgsisa vkpvppsaas keapsasttt tttttppsev pvkkdsiree arieaastdt
     2281 qdsvassntt qngegalltp kveaptlqss qhnsgggsss sgggggksef lssltsrlsv
     2341 dppldmaspv hhgfllgtpp pllerssstt ptpmtstpsa gssgkranrt rftdyqikvl
     2401 qeffetnayp kdddleylsk llnlsprviv vwfqnarqka rkvyenqpps apeddgsgrf
     2461 qrtpglnyqc kkclqvfqry yelikhqkts cfkdenplaa qlkaaasgsg tgtgssgggs
     2521 gddrslvgas npssprtpep pckspqngsy rcdkcslvfp rfdlwrehqi vhimnpnlfp
     2581 nyppnssfgi lqyegqqqqq qialpspqqq qqqqqqqpwk rkleeeqplq lqqqqdskdl
     2641 tgepprdkrl rttilpeqld ylyqkyqmes npsrkmleni ardvglkkrv vqvwfqntra
     2701 rerkgqfrah qqvihkrcpf cralfkarsa leshlatrha dlyskgelni dsfpdgdads
     2761 npgtptssas eeskaispsa gsdlvhstmk kyyedslkky ldemtgaptd lsvkpkanka
     2821 disggeapld lskplrldsd rsgdhlsvse ksmdlrigvs ddarsethse stdnmdgdds
     2881 yfesnptspl glhgaggqqp srpvsgsgkr frtqmttvql kvmksifady ktpsmaecem
     2941 lgreiglpkr vvqvwfqnar akekksklaf aktfgqeiep pkppeecslc svkynhqfsn
     3001 tsmqdhlfsk rhidalrnhi dnikkmtddg aperaadadk apslvqqlqm lgqglppsfa
     3061 lpspkedakk gdepkkddap ppppdslapy lyaaglpgyy paaagvpgaf ihptlfsgrn
     3121 kl
//
LOCUS       XP_077503113             231 aa            linear   INV 25-NOV-2025
DEFINITION  thiamine-triphosphatase-like [Amblyomma americanum].
ACCESSION   XP_077503113
VERSION     XP_077503113.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646987.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..231
                     /product="thiamine-triphosphatase-like"
                     /calculated_mol_wt=25712
     Region          12..205
                     /region_name="CYTH-like_Pase"
                     /note="CYTH-like (also known as triphosphate tunnel
                     metalloenzyme (TTM)-like) Phosphatases; cl11964"
                     /db_xref="CDD:448368"
     Site            order(12,14,16,44,60,62,64,71,88,132,134,148,158,160)
                     /site_type="other"
                     /note="putative active site"
                     /db_xref="CDD:143625"
     Site            order(12,14,146,158,160)
                     /site_type="other"
                     /note="putative metal binding residues [ion binding]"
                     /db_xref="CDD:143625"
     Site            order(12,14,16)
                     /site_type="active"
                     /note="signature motif [active]"
                     /db_xref="CDD:143625"
     Site            order(14,16,44,60,64,71,88,134,158)
                     /site_type="other"
                     /note="putative triphosphate binding site [ion binding]"
                     /db_xref="CDD:143625"
     CDS             1..231
                     /gene="LOC144113715"
                     /coded_by="XM_077646987.1:254..949"
                     /db_xref="GeneID:144113715"
ORIGIN      
        1 magaeparcc reverkfkvp pdieqrllsl gaelvsqkaf vdtyydtedc slclrdhwlr
       61 lrseknawel kyrrkedasc gaatayreeq gdvriletlr allpeaglek gatqvrdlva
      121 netlrelavi ktvrrtfkmp tnavvdidet dwglsvgeie ivlheadaea lagavqnata
      181 eiaqlaaqlg vqdgpppegk vesylrlqrp alyrrlvedi wphakakdac v
//
LOCUS       XP_077503114             231 aa            linear   INV 25-NOV-2025
DEFINITION  thiamine-triphosphatase-like [Amblyomma americanum].
ACCESSION   XP_077503114
VERSION     XP_077503114.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646988.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..231
                     /product="thiamine-triphosphatase-like"
                     /calculated_mol_wt=25712
     Region          12..205
                     /region_name="CYTH-like_Pase"
                     /note="CYTH-like (also known as triphosphate tunnel
                     metalloenzyme (TTM)-like) Phosphatases; cl11964"
                     /db_xref="CDD:448368"
     Site            order(12,14,16,44,60,62,64,71,88,132,134,148,158,160)
                     /site_type="other"
                     /note="putative active site"
                     /db_xref="CDD:143625"
     Site            order(12,14,146,158,160)
                     /site_type="other"
                     /note="putative metal binding residues [ion binding]"
                     /db_xref="CDD:143625"
     Site            order(12,14,16)
                     /site_type="active"
                     /note="signature motif [active]"
                     /db_xref="CDD:143625"
     Site            order(14,16,44,60,64,71,88,134,158)
                     /site_type="other"
                     /note="putative triphosphate binding site [ion binding]"
                     /db_xref="CDD:143625"
     CDS             1..231
                     /gene="LOC144113715"
                     /coded_by="XM_077646988.1:256..951"
                     /db_xref="GeneID:144113715"
ORIGIN      
        1 magaeparcc reverkfkvp pdieqrllsl gaelvsqkaf vdtyydtedc slclrdhwlr
       61 lrseknawel kyrrkedasc gaatayreeq gdvriletlr allpeaglek gatqvrdlva
      121 netlrelavi ktvrrtfkmp tnavvdidet dwglsvgeie ivlheadaea lagavqnata
      181 eiaqlaaqlg vqdgpppegk vesylrlqrp alyrrlvedi wphakakdac v
//
LOCUS       XP_077503115             231 aa            linear   INV 25-NOV-2025
DEFINITION  thiamine-triphosphatase-like [Amblyomma americanum].
ACCESSION   XP_077503115
VERSION     XP_077503115.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646989.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..231
                     /product="thiamine-triphosphatase-like"
                     /calculated_mol_wt=25712
     Region          12..205
                     /region_name="CYTH-like_Pase"
                     /note="CYTH-like (also known as triphosphate tunnel
                     metalloenzyme (TTM)-like) Phosphatases; cl11964"
                     /db_xref="CDD:448368"
     Site            order(12,14,16,44,60,62,64,71,88,132,134,148,158,160)
                     /site_type="other"
                     /note="putative active site"
                     /db_xref="CDD:143625"
     Site            order(12,14,146,158,160)
                     /site_type="other"
                     /note="putative metal binding residues [ion binding]"
                     /db_xref="CDD:143625"
     Site            order(12,14,16)
                     /site_type="active"
                     /note="signature motif [active]"
                     /db_xref="CDD:143625"
     Site            order(14,16,44,60,64,71,88,134,158)
                     /site_type="other"
                     /note="putative triphosphate binding site [ion binding]"
                     /db_xref="CDD:143625"
     CDS             1..231
                     /gene="LOC144113715"
                     /coded_by="XM_077646989.1:787..1482"
                     /db_xref="GeneID:144113715"
ORIGIN      
        1 magaeparcc reverkfkvp pdieqrllsl gaelvsqkaf vdtyydtedc slclrdhwlr
       61 lrseknawel kyrrkedasc gaatayreeq gdvriletlr allpeaglek gatqvrdlva
      121 netlrelavi ktvrrtfkmp tnavvdidet dwglsvgeie ivlheadaea lagavqnata
      181 eiaqlaaqlg vqdgpppegk vesylrlqrp alyrrlvedi wphakakdac v
//
LOCUS       XP_077503116             231 aa            linear   INV 25-NOV-2025
DEFINITION  thiamine-triphosphatase-like [Amblyomma americanum].
ACCESSION   XP_077503116
VERSION     XP_077503116.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646990.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..231
                     /product="thiamine-triphosphatase-like"
                     /calculated_mol_wt=25712
     Region          12..205
                     /region_name="CYTH-like_Pase"
                     /note="CYTH-like (also known as triphosphate tunnel
                     metalloenzyme (TTM)-like) Phosphatases; cl11964"
                     /db_xref="CDD:448368"
     Site            order(12,14,16,44,60,62,64,71,88,132,134,148,158,160)
                     /site_type="other"
                     /note="putative active site"
                     /db_xref="CDD:143625"
     Site            order(12,14,146,158,160)
                     /site_type="other"
                     /note="putative metal binding residues [ion binding]"
                     /db_xref="CDD:143625"
     Site            order(12,14,16)
                     /site_type="active"
                     /note="signature motif [active]"
                     /db_xref="CDD:143625"
     Site            order(14,16,44,60,64,71,88,134,158)
                     /site_type="other"
                     /note="putative triphosphate binding site [ion binding]"
                     /db_xref="CDD:143625"
     CDS             1..231
                     /gene="LOC144113715"
                     /coded_by="XM_077646990.1:391..1086"
                     /db_xref="GeneID:144113715"
ORIGIN      
        1 magaeparcc reverkfkvp pdieqrllsl gaelvsqkaf vdtyydtedc slclrdhwlr
       61 lrseknawel kyrrkedasc gaatayreeq gdvriletlr allpeaglek gatqvrdlva
      121 netlrelavi ktvrrtfkmp tnavvdidet dwglsvgeie ivlheadaea lagavqnata
      181 eiaqlaaqlg vqdgpppegk vesylrlqrp alyrrlvedi wphakakdac v
//
LOCUS       XP_077503117             231 aa            linear   INV 25-NOV-2025
DEFINITION  thiamine-triphosphatase-like [Amblyomma americanum].
ACCESSION   XP_077503117
VERSION     XP_077503117.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646991.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..231
                     /product="thiamine-triphosphatase-like"
                     /calculated_mol_wt=25712
     Region          12..205
                     /region_name="CYTH-like_Pase"
                     /note="CYTH-like (also known as triphosphate tunnel
                     metalloenzyme (TTM)-like) Phosphatases; cl11964"
                     /db_xref="CDD:448368"
     Site            order(12,14,16,44,60,62,64,71,88,132,134,148,158,160)
                     /site_type="other"
                     /note="putative active site"
                     /db_xref="CDD:143625"
     Site            order(12,14,146,158,160)
                     /site_type="other"
                     /note="putative metal binding residues [ion binding]"
                     /db_xref="CDD:143625"
     Site            order(12,14,16)
                     /site_type="active"
                     /note="signature motif [active]"
                     /db_xref="CDD:143625"
     Site            order(14,16,44,60,64,71,88,134,158)
                     /site_type="other"
                     /note="putative triphosphate binding site [ion binding]"
                     /db_xref="CDD:143625"
     CDS             1..231
                     /gene="LOC144113715"
                     /coded_by="XM_077646991.1:442..1137"
                     /db_xref="GeneID:144113715"
ORIGIN      
        1 magaeparcc reverkfkvp pdieqrllsl gaelvsqkaf vdtyydtedc slclrdhwlr
       61 lrseknawel kyrrkedasc gaatayreeq gdvriletlr allpeaglek gatqvrdlva
      121 netlrelavi ktvrrtfkmp tnavvdidet dwglsvgeie ivlheadaea lagavqnata
      181 eiaqlaaqlg vqdgpppegk vesylrlqrp alyrrlvedi wphakakdac v
//
LOCUS       XP_077503118             231 aa            linear   INV 25-NOV-2025
DEFINITION  thiamine-triphosphatase-like [Amblyomma americanum].
ACCESSION   XP_077503118
VERSION     XP_077503118.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646992.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..231
                     /product="thiamine-triphosphatase-like"
                     /calculated_mol_wt=25712
     Region          12..205
                     /region_name="CYTH-like_Pase"
                     /note="CYTH-like (also known as triphosphate tunnel
                     metalloenzyme (TTM)-like) Phosphatases; cl11964"
                     /db_xref="CDD:448368"
     Site            order(12,14,16,44,60,62,64,71,88,132,134,148,158,160)
                     /site_type="other"
                     /note="putative active site"
                     /db_xref="CDD:143625"
     Site            order(12,14,146,158,160)
                     /site_type="other"
                     /note="putative metal binding residues [ion binding]"
                     /db_xref="CDD:143625"
     Site            order(12,14,16)
                     /site_type="active"
                     /note="signature motif [active]"
                     /db_xref="CDD:143625"
     Site            order(14,16,44,60,64,71,88,134,158)
                     /site_type="other"
                     /note="putative triphosphate binding site [ion binding]"
                     /db_xref="CDD:143625"
     CDS             1..231
                     /gene="LOC144113715"
                     /coded_by="XM_077646992.1:44..739"
                     /db_xref="GeneID:144113715"
ORIGIN      
        1 magaeparcc reverkfkvp pdieqrllsl gaelvsqkaf vdtyydtedc slclrdhwlr
       61 lrseknawel kyrrkedasc gaatayreeq gdvriletlr allpeaglek gatqvrdlva
      121 netlrelavi ktvrrtfkmp tnavvdidet dwglsvgeie ivlheadaea lagavqnata
      181 eiaqlaaqlg vqdgpppegk vesylrlqrp alyrrlvedi wphakakdac v
//
LOCUS       XP_077503119            1041 aa            linear   INV 25-NOV-2025
DEFINITION  WD repeat domain 59 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503119
VERSION     XP_077503119.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646993.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1041
                     /product="WD repeat domain 59 isoform X1"
                     /calculated_mol_wt=116037
     Region          90..410
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          92..129
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          134..172
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          180..217
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          223..259
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          265..292
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          309..351
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          377..414
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          480..566
                     /region_name="RWD"
                     /note="RWD domain; cl02687"
                     /db_xref="CDD:413438"
     Region          987..1033
                     /region_name="mRING-H2-C3H3C2_WDR59"
                     /note="Modified RING finger, H2 subclass (C3H3C2-type),
                     found in WD repeat-containing protein 59 (WDR59) and
                     similar proteins; cd16692"
                     /db_xref="CDD:438353"
     CDS             1..1041
                     /gene="Wdr59"
                     /coded_by="XM_077646993.1:16..3141"
                     /db_xref="GeneID:144113717"
ORIGIN      
        1 mnasscstgl pcrrskalvm pvsrrvmfgv kycsrevveq prirrrrgcc cggdggnrpl
       61 lgeslpqpqq tltmeavnlt yvvsehkdlq atamaldsdg qyavlagrry ialvnldspd
      121 ttvkksvrqs kfevstaewn phladtflla cnqnveiftw cngellskqt iskahtrais
      181 dinwspfdes llasssydty vhiwdsrdlr kpylslsavt gasqvkwnki tqhvlatshd
      241 gdvriwdtrk sgspvqyiaa hsskiygldw sptsenqlat ssqdgtvkfw dvtnpkrvef
      301 mlqagapvwr arylpfgdgl vtalvpklrk gdknlviwss lrtgmpirtf lshsdvvlef
      361 gwrrprqgeq nkqsdlgipa ffsecrdyql vtwskdqslh lwhvdtkhqv cgqdvsedds
      421 vsltasmvae vpttpissef pkfigtvssk getplpqhpp pdlerefsll ninipkviiq
      481 eldaekrvcc vaitvgmhvl rlrlqfpqly pynasptfqf lkgttvdssm enrlakvlrm
      541 tseqhvkrnr nclepclrql vatlescves aevedsdsgy laerlkhpya aygsfqdssv
      601 pfprtsgarf cgadylvcft rpshlqkmna ptevtprsls algaylasqh kasrppstss
      661 lvslyaphsp gtatvqpqdv sissfyyker kgrgrtqrrt grqtssqqhp mpsaavliyn
      721 msgimpinqs laqnymldwg dpvaacnhna tcaanvgrkd laqvwslaal iaspqlsqdk
      781 qgpdaavpwa qhsfgrrmle siishylnhy diqtvamlcc ifsgenangn cgkkfafdqa
      841 cnsqltgsww qkvggspyht vtgpsdavfs glyveslkrn rsnswsdsid dirfmdsspd
      901 prevertnhr ancklldptk sahydefkkk yaeilykwkl yeqracvlkc lstsppkvka
      961 vdfitvchyc ksdvrsvqcd eckkltlhci vchlavrgql hfcvscghgg hvehlldwfr
     1021 tqsqcpsgcg crclaegevf a
//
LOCUS       XP_077503120            1032 aa            linear   INV 25-NOV-2025
DEFINITION  WD repeat domain 59 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503120
VERSION     XP_077503120.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646994.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1032
                     /product="WD repeat domain 59 isoform X2"
                     /calculated_mol_wt=114922
     Region          90..410
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          92..129
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          134..172
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          180..217
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          223..259
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          265..292
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          309..351
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          377..414
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          480..566
                     /region_name="RWD"
                     /note="RWD domain; cl02687"
                     /db_xref="CDD:413438"
     Region          978..1024
                     /region_name="mRING-H2-C3H3C2_WDR59"
                     /note="Modified RING finger, H2 subclass (C3H3C2-type),
                     found in WD repeat-containing protein 59 (WDR59) and
                     similar proteins; cd16692"
                     /db_xref="CDD:438353"
     CDS             1..1032
                     /gene="Wdr59"
                     /coded_by="XM_077646994.1:16..3114"
                     /db_xref="GeneID:144113717"
ORIGIN      
        1 mnasscstgl pcrrskalvm pvsrrvmfgv kycsrevveq prirrrrgcc cggdggnrpl
       61 lgeslpqpqq tltmeavnlt yvvsehkdlq atamaldsdg qyavlagrry ialvnldspd
      121 ttvkksvrqs kfevstaewn phladtflla cnqnveiftw cngellskqt iskahtrais
      181 dinwspfdes llasssydty vhiwdsrdlr kpylslsavt gasqvkwnki tqhvlatshd
      241 gdvriwdtrk sgspvqyiaa hsskiygldw sptsenqlat ssqdgtvkfw dvtnpkrvef
      301 mlqagapvwr arylpfgdgl vtalvpklrk gdknlviwss lrtgmpirtf lshsdvvlef
      361 gwrrprqgeq nkqsdlgipa ffsecrdyql vtwskdqslh lwhvdtkhqv cgqdvsedds
      421 vsltasmvae vpttpissef pkfigtvssk getplpqhpp pdlerefsll ninipkviiq
      481 eldaekrvcc vaitvgmhvl rlrlqfpqly pynasptfqf lkgttvdssm enrlakvlrm
      541 tseqhvkrnr nclepclrql vatlescves aevedsdsgy laerlkhpya aygsfqdssv
      601 pfprtsgarf cgadylvcft rpshlqkmna ptevtprsls algaylasqh kasrppstss
      661 lvslyaphsp gtatvqpqdv sissfyyker kgrgrtqrrt grqtssqqhp mpsaavliyn
      721 msgimpinqs laqnymldwg dpvaacnhna tcaanvgrkd laqvwslaal iaspqlsqdk
      781 qgpdaavpwa qhsfgrrmle siishylnhy diqtvamlcc ifsgenangn cgkkfafdqa
      841 cnsvggspyh tvtgpsdavf sglyveslkr nrsnswsdsi ddirfmdssp dprevertnh
      901 rancklldpt ksahydefkk kyaeilykwk lyeqracvlk clstsppkvk avdfitvchy
      961 cksdvrsvqc deckkltlhc ivchlavrgq lhfcvscghg ghvehlldwf rtqsqcpsgc
     1021 gcrclaegev fa
//
LOCUS       XP_077503121             704 aa            linear   INV 25-NOV-2025
DEFINITION  tRNA (cytosine(34)-C(5))-methyltransferase Nsun2 isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077503121
VERSION     XP_077503121.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646995.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..704
                     /product="tRNA (cytosine(34)-C(5))-methyltransferase Nsun2
                     isoform X1"
                     /calculated_mol_wt=80191
     Region          <39..423
                     /region_name="RsmB"
                     /note="16S rRNA C967 or C1407 C5-methylase, RsmB/RsmF
                     family [Translation, ribosomal structure and biogenesis];
                     COG0144"
                     /db_xref="CDD:439914"
     CDS             1..704
                     /gene="Nsun2"
                     /coded_by="XM_077646995.1:146..2260"
                     /db_xref="GeneID:144113719"
ORIGIN      
        1 mgrkrqrkrq gkapraakpq kvsenggppl yteidrsnea fekyykaqqi vpeeeweefm
       61 salrrglpva fritgflpes khllkiiksq yfadllnvgg deekvgkpsn ltwypdelaw
      121 qldlsrimir kseayarlhs flisetdtgn itrqeavsmi ppllldvqph hkvldmcaap
      181 gsktaqvmem lhghvntipe gvvvandvdn krcymlvhqa krlhstcclv tnhdaaampn
      241 myiskedgtl evlkydrilc dvpcsgdgtl rknidlwrkw nvangnsihg lqvriarrgl
      301 ellveggvmv ystcsmnple neavitslll qcegavelvd arsslpglvs spglhtwkvs
      361 skdmavytsf eevpetyhtq irahmfppsp evaaqlkler cirilphqqd tgaffvavlk
      421 ktasklpwes qnkdkgvara seeceeqqdt kedksivprk krriqgyked pfvflsednk
      481 iwkavrdfye vdpsfpnqql lgrcqqenkr niylvsktvr niielnseki kiintgvraf
      541 crcegkeela cdfrvcqegl ptvlpylkkr klamcrqdly vllteeyple tqfspelqeq
      601 lkdlekgcvl lvytsfpgtp defqlvlagr rgkatlrcyv akqervhylr vcgydvseye
      661 krdrrkkped tpkietlepe eaveyaeetm qeqtendikv ggdc
//
LOCUS       XP_077503122             697 aa            linear   INV 25-NOV-2025
DEFINITION  tRNA (cytosine(34)-C(5))-methyltransferase Nsun2 isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077503122
VERSION     XP_077503122.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646996.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..697
                     /product="tRNA (cytosine(34)-C(5))-methyltransferase Nsun2
                     isoform X2"
                     /calculated_mol_wt=79521
     Region          <39..423
                     /region_name="RsmB"
                     /note="16S rRNA C967 or C1407 C5-methylase, RsmB/RsmF
                     family [Translation, ribosomal structure and biogenesis];
                     COG0144"
                     /db_xref="CDD:439914"
     CDS             1..697
                     /gene="Nsun2"
                     /coded_by="XM_077646996.1:146..2239"
                     /db_xref="GeneID:144113719"
ORIGIN      
        1 mgrkrqrkrq gkapraakpq kvsenggppl yteidrsnea fekyykaqqi vpeeeweefm
       61 salrrglpva fritgflpes khllkiiksq yfadllnvgg deekvgkpsn ltwypdelaw
      121 qldlsrimir kseayarlhs flisetdtgn itrqeavsmi ppllldvqph hkvldmcaap
      181 gsktaqvmem lhghvntipe gvvvandvdn krcymlvhqa krlhstcclv tnhdaaampn
      241 myiskedgtl evlkydrilc dvpcsgdgtl rknidlwrkw nvangnsihg lqvriarrgl
      301 ellveggvmv ystcsmnple neavitslll qcegavelvd arsslpglvs spglhtwkvs
      361 skdmavytsf eevpetyhtq irahmfppsp evaaqlkler cirilphqqd tgaffvavlk
      421 ktasklpwes qnkdkeceeq qdtkedksiv prkkrriqgy kedpfvflse dnkiwkavrd
      481 fyevdpsfpn qqllgrcqqe nkrniylvsk tvrniielns ekikiintgv rafcrcegke
      541 elacdfrvcq eglptvlpyl kkrklamcrq dlyvllteey pletqfspel qeqlkdlekg
      601 cvllvytsfp gtpdefqlvl agrrgkatlr cyvakqervh ylrvcgydvs eyekrdrrkk
      661 pedtpkietl epeeaveyae etmqeqtend ikvggdc
//
LOCUS       XP_077503123             689 aa            linear   INV 25-NOV-2025
DEFINITION  tRNA (cytosine(34)-C(5))-methyltransferase Nsun2 isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077503123
VERSION     XP_077503123.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646997.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..689
                     /product="tRNA (cytosine(34)-C(5))-methyltransferase Nsun2
                     isoform X3"
                     /calculated_mol_wt=78526
     Region          <39..423
                     /region_name="RsmB"
                     /note="16S rRNA C967 or C1407 C5-methylase, RsmB/RsmF
                     family [Translation, ribosomal structure and biogenesis];
                     COG0144"
                     /db_xref="CDD:439914"
     CDS             1..689
                     /gene="Nsun2"
                     /coded_by="XM_077646997.1:146..2215"
                     /db_xref="GeneID:144113719"
ORIGIN      
        1 mgrkrqrkrq gkapraakpq kvsenggppl yteidrsnea fekyykaqqi vpeeeweefm
       61 salrrglpva fritgflpes khllkiiksq yfadllnvgg deekvgkpsn ltwypdelaw
      121 qldlsrimir kseayarlhs flisetdtgn itrqeavsmi ppllldvqph hkvldmcaap
      181 gsktaqvmem lhghvntipe gvvvandvdn krcymlvhqa krlhstcclv tnhdaaampn
      241 myiskedgtl evlkydrilc dvpcsgdgtl rknidlwrkw nvangnsihg lqvriarrgl
      301 ellveggvmv ystcsmnple neavitslll qcegavelvd arsslpglvs spglhtwkvs
      361 skdmavytsf eevpetyhtq irahmfppsp evaaqlkler cirilphqqd tgaffvavlk
      421 ktasklpwes qnkdkgvara seeceeqqdt kedksivprk krriqgyked pfvflsednk
      481 iwkavrdfye vdpsfpnqql lgrcqqenkr niylvsktvr niielnseki kiintgvraf
      541 crcegkeela cdfrvcqegl ptvlpylkkr klamcrqdly vllteeyple tqfspelqeq
      601 lkdlekgcvl lvytsfpgtp defqlvlagr rgkatlrcyv akqervhylr vcgydvseye
      661 krdrrkkped tpkietlepe eawmdgvlc
//
LOCUS       XP_077503124             687 aa            linear   INV 25-NOV-2025
DEFINITION  tRNA (cytosine(34)-C(5))-methyltransferase Nsun2 isoform X4
            [Amblyomma americanum].
ACCESSION   XP_077503124
VERSION     XP_077503124.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077646998.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..687
                     /product="tRNA (cytosine(34)-C(5))-methyltransferase Nsun2
                     isoform X4"
                     /calculated_mol_wt=78279
     Region          <39..423
                     /region_name="RsmB"
                     /note="16S rRNA C967 or C1407 C5-methylase, RsmB/RsmF
                     family [Translation, ribosomal structure and biogenesis];
                     COG0144"
                     /db_xref="CDD:439914"
     CDS             1..687
                     /gene="Nsun2"
                     /coded_by="XM_077646998.1:146..2209"
                     /db_xref="GeneID:144113719"
ORIGIN      
        1 mgrkrqrkrq gkapraakpq kvsenggppl yteidrsnea fekyykaqqi vpeeeweefm
       61 salrrglpva fritgflpes khllkiiksq yfadllnvgg deekvgkpsn ltwypdelaw
      121 qldlsrimir kseayarlhs flisetdtgn itrqeavsmi ppllldvqph hkvldmcaap
      181 gsktaqvmem lhghvntipe gvvvandvdn krcymlvhqa krlhstcclv tnhdaaampn
      241 myiskedgtl evlkydrilc dvpcsgdgtl rknidlwrkw nvangnsihg lqvriarrgl
      301 ellveggvmv ystcsmnple neavitslll qcegavelvd arsslpglvs spglhtwkvs
      361 skdmavytsf eevpetyhtq irahmfppsp evaaqlkler cirilphqqd tgaffvavlk
      421 ktasklpwes qnkdkgvara seeceeqqdt kedksivprk krriqgyked pfvflsednk
      481 iwkavrdfye vdpsfpnqql lgrcqqenkr niylvsktvr niielnseki kiintgvraf
      541 crcegkeela cdfrvcqegl ptvlpylkkr klamcrqdly vllteeyple tqfspelqeq
      601 lkdlekgcvl lvytsfpgtp defqlvlagr rgkatlrcyv akqervhylr vcgydvseye
      661 krdrrkkped tpkietlepe earclsv
//
LOCUS       XP_077503126             682 aa            linear   INV 25-NOV-2025
DEFINITION  tRNA (cytosine(34)-C(5))-methyltransferase Nsun2 isoform X5
            [Amblyomma americanum].
ACCESSION   XP_077503126
VERSION     XP_077503126.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647000.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..682
                     /product="tRNA (cytosine(34)-C(5))-methyltransferase Nsun2
                     isoform X5"
                     /calculated_mol_wt=77855
     Region          <39..423
                     /region_name="RsmB"
                     /note="16S rRNA C967 or C1407 C5-methylase, RsmB/RsmF
                     family [Translation, ribosomal structure and biogenesis];
                     COG0144"
                     /db_xref="CDD:439914"
     CDS             1..682
                     /gene="Nsun2"
                     /coded_by="XM_077647000.1:146..2194"
                     /db_xref="GeneID:144113719"
ORIGIN      
        1 mgrkrqrkrq gkapraakpq kvsenggppl yteidrsnea fekyykaqqi vpeeeweefm
       61 salrrglpva fritgflpes khllkiiksq yfadllnvgg deekvgkpsn ltwypdelaw
      121 qldlsrimir kseayarlhs flisetdtgn itrqeavsmi ppllldvqph hkvldmcaap
      181 gsktaqvmem lhghvntipe gvvvandvdn krcymlvhqa krlhstcclv tnhdaaampn
      241 myiskedgtl evlkydrilc dvpcsgdgtl rknidlwrkw nvangnsihg lqvriarrgl
      301 ellveggvmv ystcsmnple neavitslll qcegavelvd arsslpglvs spglhtwkvs
      361 skdmavytsf eevpetyhtq irahmfppsp evaaqlkler cirilphqqd tgaffvavlk
      421 ktasklpwes qnkdkeceeq qdtkedksiv prkkrriqgy kedpfvflse dnkiwkavrd
      481 fyevdpsfpn qqllgrcqqe nkrniylvsk tvrniielns ekikiintgv rafcrcegke
      541 elacdfrvcq eglptvlpyl kkrklamcrq dlyvllteey pletqfspel qeqlkdlekg
      601 cvllvytsfp gtpdefqlvl agrrgkatlr cyvakqervh ylrvcgydvs eyekrdrrkk
      661 pedtpkietl epeeawmdgv lc
//
LOCUS       XP_077503127             227 aa            linear   INV 25-NOV-2025
DEFINITION  pre-mRNA-splicing factor SYF2 [Amblyomma americanum].
ACCESSION   XP_077503127
VERSION     XP_077503127.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647001.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..227
                     /product="pre-mRNA-splicing factor SYF2"
                     /calculated_mol_wt=27216
     Region          74..222
                     /region_name="SYF2"
                     /note="SYF2 splicing factor; pfam08231"
                     /db_xref="CDD:462400"
     CDS             1..227
                     /gene="LOC144113720"
                     /coded_by="XM_077647001.1:135..818"
                     /db_xref="GeneID:144113720"
ORIGIN      
        1 msegqpsasm sskrseqler lralhmrrne artlnhkevi eedkksklpa nweskqkwtr
       61 ykqeeeekye daakrgedys rikllnvsad eaerldrkkk rknpdvgfsd yeaatvrqyq
      121 rlvkqikpdf eqyerkkeem gdalyptrdt iihglhkdtk egidrmvddi ekqidkrnky
      181 srrrrfndde didyinernm kfnkklerfy gkytaeikqn lergtav
//
LOCUS       XP_077503128            1214 aa            linear   INV 25-NOV-2025
DEFINITION  splicing factor 3b subunit 3 [Amblyomma americanum].
ACCESSION   XP_077503128
VERSION     XP_077503128.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647002.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1214
                     /product="splicing factor 3b subunit 3"
                     /calculated_mol_wt=134021
     Region          76..592
                     /region_name="MMS1_N"
                     /note="Mono-functional DNA-alkylating methyl
                     methanesulfonate N-term; pfam10433"
                     /db_xref="CDD:463091"
     Region          858..1179
                     /region_name="CPSF_A"
                     /note="CPSF A subunit region; pfam03178"
                     /db_xref="CDD:427182"
     CDS             1..1214
                     /gene="Sf3b3"
                     /coded_by="XM_077647002.1:248..3892"
                     /db_xref="GeneID:144113721"
ORIGIN      
        1 mylynltlqr atgithavhg nfsgtklqev avsrgkilel lrpdpntgkv ytlyasevfg
       61 virsmmsfrl tggskdylvi gsdsgrivil eynpqkscfe kvhqetfgks gcrrivpgqh
      121 lamdpkgral mvaavekqkl vyilnrdaaa rltissplea hksstlvyha vgvdvgfenp
      181 vfaclemdye eadtdptgea ahnthqtltf yeldlglnhv vrkyseplee hgnfliavpg
      241 gsdgpsgvli csenyitykn fgdqpdircp iprrrndldd pergmifvcs anhktkamff
      301 flaqteqgdi fkitletded mvteiklkyf dtipvatsmc vlktgflfva aefgnhclyq
      361 iarlgeedee pefssaiple egdtfffapr alrnllpvee ldslspamgc tiadlanedt
      421 pqlyvacgrg prsclrvlrh glevsemavs elpgnpnavw tvkrksdedy dayiivsfvn
      481 atlvlsiget veevtdsgfl gttptlscaq igddalvqvy pegirhirad krvnewrtpg
      541 kkaivkcavn qrqvvialtg gelvyfemdp tgqlneytdr remsadiicm slasvptgeq
      601 rsrflavgla dntvriisld psdclsplsm qalpatpesl sivemgmtev dtsgqgilyl
      661 niglqngvll rtvldqvtgd lsdtrtrylg srpvklfkvr mqgsdaalam ssrswlsyyy
      721 qnrfhltpls yetlefasgf sseqcpegiv aistntlril aleklgavfn qvstpleytp
      781 rkfvvntdsg hlivvetdhn aytekmkqqr kqqmaeemve aagegeqela aemaaaflse
      841 vlpeavfgsp kagsgmwasv irvlnpadgq tlckvaleqn eaalsvalvk fssqpdeqyv
      901 vvgaarelsl qpwharsggl lityrlshag etrldlvhat pveeaptalc pfqgrvlagv
      961 gkclrlydlg rkkllrkcen kyipnaivti qcmgnrvvvs dvqesffflr ykrlenqlvi
     1021 faddsvprwi tascmldydt iagadkfgnv sivrlpssis devdedptgi kslwdrgwlg
     1081 gssqkaevia nfhvgetvls lqkatlipgg seslvfvtls gtigvlvpft ahedhdffqh
     1141 lemhmrsenp plcgrdhlsf rssyfpvknv idgdlceqfn sldpskqksi aeeldrnpse
     1201 vskkledirt ryaf
//
LOCUS       XP_077503129            1214 aa            linear   INV 25-NOV-2025
DEFINITION  splicing factor 3b subunit 3 [Amblyomma americanum].
ACCESSION   XP_077503129
VERSION     XP_077503129.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647003.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1214
                     /product="splicing factor 3b subunit 3"
                     /calculated_mol_wt=134021
     Region          76..592
                     /region_name="MMS1_N"
                     /note="Mono-functional DNA-alkylating methyl
                     methanesulfonate N-term; pfam10433"
                     /db_xref="CDD:463091"
     Region          858..1179
                     /region_name="CPSF_A"
                     /note="CPSF A subunit region; pfam03178"
                     /db_xref="CDD:427182"
     CDS             1..1214
                     /gene="Sf3b3"
                     /coded_by="XM_077647003.1:227..3871"
                     /db_xref="GeneID:144113721"
ORIGIN      
        1 mylynltlqr atgithavhg nfsgtklqev avsrgkilel lrpdpntgkv ytlyasevfg
       61 virsmmsfrl tggskdylvi gsdsgrivil eynpqkscfe kvhqetfgks gcrrivpgqh
      121 lamdpkgral mvaavekqkl vyilnrdaaa rltissplea hksstlvyha vgvdvgfenp
      181 vfaclemdye eadtdptgea ahnthqtltf yeldlglnhv vrkyseplee hgnfliavpg
      241 gsdgpsgvli csenyitykn fgdqpdircp iprrrndldd pergmifvcs anhktkamff
      301 flaqteqgdi fkitletded mvteiklkyf dtipvatsmc vlktgflfva aefgnhclyq
      361 iarlgeedee pefssaiple egdtfffapr alrnllpvee ldslspamgc tiadlanedt
      421 pqlyvacgrg prsclrvlrh glevsemavs elpgnpnavw tvkrksdedy dayiivsfvn
      481 atlvlsiget veevtdsgfl gttptlscaq igddalvqvy pegirhirad krvnewrtpg
      541 kkaivkcavn qrqvvialtg gelvyfemdp tgqlneytdr remsadiicm slasvptgeq
      601 rsrflavgla dntvriisld psdclsplsm qalpatpesl sivemgmtev dtsgqgilyl
      661 niglqngvll rtvldqvtgd lsdtrtrylg srpvklfkvr mqgsdaalam ssrswlsyyy
      721 qnrfhltpls yetlefasgf sseqcpegiv aistntlril aleklgavfn qvstpleytp
      781 rkfvvntdsg hlivvetdhn aytekmkqqr kqqmaeemve aagegeqela aemaaaflse
      841 vlpeavfgsp kagsgmwasv irvlnpadgq tlckvaleqn eaalsvalvk fssqpdeqyv
      901 vvgaarelsl qpwharsggl lityrlshag etrldlvhat pveeaptalc pfqgrvlagv
      961 gkclrlydlg rkkllrkcen kyipnaivti qcmgnrvvvs dvqesffflr ykrlenqlvi
     1021 faddsvprwi tascmldydt iagadkfgnv sivrlpssis devdedptgi kslwdrgwlg
     1081 gssqkaevia nfhvgetvls lqkatlipgg seslvfvtls gtigvlvpft ahedhdffqh
     1141 lemhmrsenp plcgrdhlsf rssyfpvknv idgdlceqfn sldpskqksi aeeldrnpse
     1201 vskkledirt ryaf
//
LOCUS       XP_077503130            1214 aa            linear   INV 25-NOV-2025
DEFINITION  splicing factor 3b subunit 3 [Amblyomma americanum].
ACCESSION   XP_077503130
VERSION     XP_077503130.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647004.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1214
                     /product="splicing factor 3b subunit 3"
                     /calculated_mol_wt=134021
     Region          76..592
                     /region_name="MMS1_N"
                     /note="Mono-functional DNA-alkylating methyl
                     methanesulfonate N-term; pfam10433"
                     /db_xref="CDD:463091"
     Region          858..1179
                     /region_name="CPSF_A"
                     /note="CPSF A subunit region; pfam03178"
                     /db_xref="CDD:427182"
     CDS             1..1214
                     /gene="Sf3b3"
                     /coded_by="XM_077647004.1:207..3851"
                     /db_xref="GeneID:144113721"
ORIGIN      
        1 mylynltlqr atgithavhg nfsgtklqev avsrgkilel lrpdpntgkv ytlyasevfg
       61 virsmmsfrl tggskdylvi gsdsgrivil eynpqkscfe kvhqetfgks gcrrivpgqh
      121 lamdpkgral mvaavekqkl vyilnrdaaa rltissplea hksstlvyha vgvdvgfenp
      181 vfaclemdye eadtdptgea ahnthqtltf yeldlglnhv vrkyseplee hgnfliavpg
      241 gsdgpsgvli csenyitykn fgdqpdircp iprrrndldd pergmifvcs anhktkamff
      301 flaqteqgdi fkitletded mvteiklkyf dtipvatsmc vlktgflfva aefgnhclyq
      361 iarlgeedee pefssaiple egdtfffapr alrnllpvee ldslspamgc tiadlanedt
      421 pqlyvacgrg prsclrvlrh glevsemavs elpgnpnavw tvkrksdedy dayiivsfvn
      481 atlvlsiget veevtdsgfl gttptlscaq igddalvqvy pegirhirad krvnewrtpg
      541 kkaivkcavn qrqvvialtg gelvyfemdp tgqlneytdr remsadiicm slasvptgeq
      601 rsrflavgla dntvriisld psdclsplsm qalpatpesl sivemgmtev dtsgqgilyl
      661 niglqngvll rtvldqvtgd lsdtrtrylg srpvklfkvr mqgsdaalam ssrswlsyyy
      721 qnrfhltpls yetlefasgf sseqcpegiv aistntlril aleklgavfn qvstpleytp
      781 rkfvvntdsg hlivvetdhn aytekmkqqr kqqmaeemve aagegeqela aemaaaflse
      841 vlpeavfgsp kagsgmwasv irvlnpadgq tlckvaleqn eaalsvalvk fssqpdeqyv
      901 vvgaarelsl qpwharsggl lityrlshag etrldlvhat pveeaptalc pfqgrvlagv
      961 gkclrlydlg rkkllrkcen kyipnaivti qcmgnrvvvs dvqesffflr ykrlenqlvi
     1021 faddsvprwi tascmldydt iagadkfgnv sivrlpssis devdedptgi kslwdrgwlg
     1081 gssqkaevia nfhvgetvls lqkatlipgg seslvfvtls gtigvlvpft ahedhdffqh
     1141 lemhmrsenp plcgrdhlsf rssyfpvknv idgdlceqfn sldpskqksi aeeldrnpse
     1201 vskkledirt ryaf
//
LOCUS       XP_077503131             801 aa            linear   INV 25-NOV-2025
DEFINITION  AFG3 like matrix AAA peptidase subunit 2 [Amblyomma americanum].
ACCESSION   XP_077503131
VERSION     XP_077503131.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647005.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..801
                     /product="AFG3 like matrix AAA peptidase subunit 2"
                     /calculated_mol_wt=89002
     Region          149..742
                     /region_name="HflB"
                     /note="ATP-dependent Zn proteases [Posttranslational
                     modification, protein turnover, chaperones]; COG0465"
                     /db_xref="CDD:440233"
     CDS             1..801
                     /gene="Afg3l2"
                     /coded_by="XM_077647005.1:220..2625"
                     /db_xref="GeneID:144113723"
ORIGIN      
        1 masrylqmgr rlernllrtw hgarlsrhei ctkiltlngv pptlpplhfl leqwqllcek
       61 ppkgfekyfp gskkgqqkea geqavdkpag kelpkdapsq sgpqtkqssg skppqwnfsf
      121 ggqgnrsgrn pfnpddqnrm mtvalvttlg ilgllafnem rykeitwkdf vnaylargiv
      181 eklevinkkw vrvrllpgnq idgnnvlwfn igsvdtfern lenvqlefni eppnfvpviy
      241 knemdgssvi gilptlllfg flfwtmrrsa gfmggggarr gggifgmget taklinpndi
      301 gvkfkdvagc eeakveimef vnflknpqqy ielgakipkg ailtgppgtg ktllakatag
      361 eanvpfitvs gseflemfvg vgpsrvrdmf smarknapci lfideidavg rkrggrsfgg
      421 hseqentlnq llvemdgfnt ttnvvvlaat nrvdildqal lrpgrfdrqi fvpapdikgr
      481 asifkvhlkp lktlldkmdl arkmaaltpg ftgadianvc neaaliaard lndnitmkhf
      541 eqaiervvag mekktnvlqp eekktvayhe aghavagwfl ehadpllkvs iiprgkglgy
      601 aqylpkeqyl ytteqlldrm cmtlggrvse qiffgkittg aqddlkkvtq sayaqvvqfg
      661 mndkvgnlsf dmpqpgdmvl dkpyseetaq iidsevrkmv qkaydrtmal ltehkedvek
      721 iaqrllekei lsrddmiell gkrpfpekst yeefvegtgs feedttlpkg leswnkgpee
      781 dsksnkpssp dkdkkqqtas s
//
LOCUS       XP_077503132             671 aa            linear   INV 25-NOV-2025
DEFINITION  nucleolar protein 9 [Amblyomma americanum].
ACCESSION   XP_077503132
VERSION     XP_077503132.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647006.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..671
                     /product="nucleolar protein 9"
                     /calculated_mol_wt=75671
     CDS             1..671
                     /gene="LOC144113724"
                     /coded_by="XM_077647006.1:345..2360"
                     /db_xref="GeneID:144113724"
ORIGIN      
        1 mkpakhsvhr lrnvsdgkgi avrrsgsdyn vglpekasrg hfshsrdsns hlsnsfqqfs
       61 pmhqvdkdtk syfmmvcerf hdgfeddedk alflenvadq tcgkelelsc ddstshcmqm
      121 lllhtrslnv ltrfmstfle hvdlmcthhi gayvmqtlle vslkylqsps vyatseqeht
      181 daeanggnqq hrhgsysard klvdrkkhsa thldasside gsqiqvptgc hdnvvlwvla
      241 vgrfclenie kfvysrvpth virsvvqvlg gcyverhlsr pqtfkkkqkt eapkealevv
      301 dipdgfyelf rtyvntiktf dlldimsnsv tsllfqalim vlqqkdpkml sslvkhvaka
      361 vfsqssderg lppafldpsa sftveemlkr cspksftklw dnhlsgillk mslhpvstfc
      421 aqrviehats seqfqemyde lepgfnelld hgqvsmlvci aaackthsvs qarfvtalmk
      481 tlhcaepedr hvlltplily mkryedydqa hqgtavvyqg svllqhlftf gktqkvaqsl
      541 lsmstealka iaccpcgshv ldafvqsdqv kkaskeelir mlkglygsla mdkygscfvm
      601 nlwdvasmaq kvdiaeelsd kesalatsvh grlvskklsl yhfihrrndw aelqktkkkt
      661 srlfqdiike k
//
LOCUS       XP_077503133             444 aa            linear   INV 25-NOV-2025
DEFINITION  LSM14 family protein trailer hitch [Amblyomma americanum].
ACCESSION   XP_077503133
VERSION     XP_077503133.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647007.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..444
                     /product="LSM14 family protein trailer hitch"
                     /calculated_mol_wt=48089
     Region          4..77
                     /region_name="LSm14_N"
                     /note="Like-Sm protein 14, N-terminal domain; cd01736"
                     /db_xref="CDD:212483"
     Site            order(12..18,20..32,35..39)
                     /site_type="other"
                     /note="Sm1 motif"
                     /db_xref="CDD:212483"
     Site            order(30,32,68)
                     /site_type="other"
                     /note="putative RNA binding site [nucleotide binding]"
                     /db_xref="CDD:212483"
     Site            64..75
                     /site_type="other"
                     /note="Sm2 motif"
                     /db_xref="CDD:212483"
     Region          283..375
                     /region_name="FDF"
                     /note="FDF domain; pfam09532"
                     /db_xref="CDD:430668"
     CDS             1..444
                     /gene="tral"
                     /coded_by="XM_077647007.1:155..1489"
                     /db_xref="GeneID:144113726"
ORIGIN      
        1 mstgipylgs kislisksei ryegilytid tkestvalak vrsfgtedrp tdrpvaprde
       61 vyeyiifrah dikdlrvsep pkpqstlpgg ltndpaivqh satpvgagag fsasaynqap
      121 apygpvgtlp sysmapqyss qpsqppaasl ggsqqsgsqs gsttpaqrks ptmdagvqvs
      181 vpptekrqsp rggsqrshgq qgsagpsrqq qhgqqhgqqh hhqqqhgqqh hqqtlqqqpq
      241 srqggaqqqr grrggvaggs sgapsgrprq gafraakpke plkfdreydf eqanaefqel
      301 enklaqtkit dekkddsgte tqvgdnhedd dvvyydknks ffdnisceai erskgvqrid
      361 wrqerklnhe tfgisannwm rrnawrgrgg ywrggrgghr ggqrgggqgg nrggarprng
      421 avprdgwssv prtdatssad aawp
//
LOCUS       XP_077503134            1016 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113727 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503134
VERSION     XP_077503134.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647008.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1016
                     /product="uncharacterized protein LOC144113727 isoform X1"
                     /calculated_mol_wt=108568
     Region          551..638
                     /region_name="TAFH"
                     /note="NHR1 homology to TAF; pfam07531"
                     /db_xref="CDD:462195"
     Region          771..1013
                     /region_name="TAF4"
                     /note="Transcription initiation factor TFIID component
                     TAF4 family; pfam05236"
                     /db_xref="CDD:461598"
     CDS             1..1016
                     /gene="LOC144113727"
                     /coded_by="XM_077647008.1:1..3051"
                     /db_xref="GeneID:144113727"
ORIGIN      
        1 mrtlffscns kmasakslee mlttdvdesv vselvgsles qlsssgihap nqeltvssat
       61 tnhvnsvpvs dglqrlphdg qkhgvaaala nscpsivvsn fgktiattsh ivgvsscass
      121 passtntvmv tlpgalpasg yinqvtgtaq qallntslvr pisnsdikiv yspqghfnaa
      181 nlaavnnavi qqrgnvkiqg vpngstairt sphtpattpg lsaihnlanv asqqtpliis
      241 qpakprivvd pkdpkaafkp qilikqeppa asvgqeflrc sspaagviqa skpgpqpvsv
      301 vtqvvsspsi lppgvqivnv npgrpgaptv vgqktlaprv vigspvrlap qmlaarpgap
      361 svqryryhrp pehdntitlq pgmmrgtllv ktengqlqvv nvapslppns taatmsgapt
      421 yrlqsvqncr hqtplvrrap ihehmkqawp hlpnlgtqpv qqlqgrktna tpmvgtvlqq
      481 gqpsinivpt vpsstpiqtl gqplpqqtvt vkvaatttta svtqplsiqt tvansqtaat
      541 tpsqmspnta kkkcknflst lirlasdqpe qvasnvkgli qglidgtiqp edftnqlqke
      601 lnsspqpclv pflkkslpyl rhslmtkelt iegvrpppqg avtlpstpai ipniqqvtgv
      661 arptmphgtt qvrvmtpttg glaaqlasta giqnvlaqhq rlvtprpatt vvapgtksll
      721 vaktsnltaa lspatvgalq tkvatpvatr tpvtalkekr tysslrdddd indvaamggv
      781 nlveesqril asnaeivgtq irsckdenfl ftnplqrrit siavkygiee ipsdvvalis
      841 hatqerlktl vqklgvvaeh rlenlktdsr yeatqdvkaq lkfleeldrl ekkrheeqer
      901 eillraaksr skmedpeqlk lkqkakemqk aemeemrqre anmtallaig prkklktens
      961 gnsgaglgag sgltnsfskl pmrprvkrvn lkdilflmeq erdsihstll yksylk
//
LOCUS       XP_077503135            1010 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113727 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503135
VERSION     XP_077503135.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647009.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1010
                     /product="uncharacterized protein LOC144113727 isoform X2"
                     /calculated_mol_wt=107866
     Region          545..632
                     /region_name="TAFH"
                     /note="NHR1 homology to TAF; pfam07531"
                     /db_xref="CDD:462195"
     Region          765..1007
                     /region_name="TAF4"
                     /note="Transcription initiation factor TFIID component
                     TAF4 family; pfam05236"
                     /db_xref="CDD:461598"
     CDS             1..1010
                     /gene="LOC144113727"
                     /coded_by="XM_077647009.1:1..3033"
                     /db_xref="GeneID:144113727"
ORIGIN      
        1 mrtlffscns kmasakslee mlttdvdesv vselvgsles qlsssgihap nqeltvssat
       61 tnhvnsvpvs dglqrlphdg qkhgvaaala nscpsivvsn fgktiattsh ivgvsscass
      121 passtntvmv tlpgalpasg yinqvtgtaq qallntslvr pisnsdikiv yspqghfnaa
      181 nlaavnnavi qqrgnvkiqg vpngstairt sphtpattpg lsaihnlanv asqqtpliis
      241 qpakprivvd pkdpkaafkp qilikqeppa asvgqeflrc sspaagviqa skpgpqpvsv
      301 vtqvvsspsi lppgvqivnv npgrpgaptv vgqktlaprv vigspvrlap qmlaarpgap
      361 svqryryhrp pehdntitlq pgmmrgtllv ktengqlqvv nvapslppns taatmsgapt
      421 yrlqsvqncr hqtplvrrap ihehmkqnlg tqpvqqlqgr ktnatpmvgt vlqqgqpsin
      481 ivptvpsstp iqtlgqplpq qtvtvkvaat tttasvtqpl siqttvansq taattpsqms
      541 pntakkkckn flstlirlas dqpeqvasnv kgliqglidg tiqpedftnq lqkelnsspq
      601 pclvpflkks lpylrhslmt keltiegvrp ppqgavtlps tpaiipniqq vtgvarptmp
      661 hgttqvrvmt pttgglaaql astagiqnvl aqhqrlvtpr pattvvapgt ksllvaktsn
      721 ltaalspatv galqtkvatp vatrtpvtal kekrtysslr ddddindvaa mggvnlvees
      781 qrilasnaei vgtqirsckd enflftnplq rritsiavky gieeipsdvv alishatqer
      841 lktlvqklgv vaehrlenlk tdsryeatqd vkaqlkflee ldrlekkrhe eqereillra
      901 aksrskmedp eqlklkqkak emqkaemeem rqreanmtal laigprkklk tensgnsgag
      961 lgagsgltns fsklpmrprv krvnlkdilf lmeqerdsih stllyksylk
//
LOCUS       XP_077503137             994 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113727 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503137
VERSION     XP_077503137.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647011.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..994
                     /product="uncharacterized protein LOC144113727 isoform X3"
                     /calculated_mol_wt=105745
     Region          529..616
                     /region_name="TAFH"
                     /note="NHR1 homology to TAF; pfam07531"
                     /db_xref="CDD:462195"
     Region          749..991
                     /region_name="TAF4"
                     /note="Transcription initiation factor TFIID component
                     TAF4 family; pfam05236"
                     /db_xref="CDD:461598"
     CDS             1..994
                     /gene="LOC144113727"
                     /coded_by="XM_077647011.1:31..3015"
                     /db_xref="GeneID:144113727"
ORIGIN      
        1 masaksleem lttdvdesvv selvgslesq lsssgihapn qeltvssatt nhvnsvpvsd
       61 glqrlphdgq khgvaaalan scpsivvsnf gktiattshi vgvsscassp asstntvmvt
      121 lpgalpasgy inqvtgtaqq allntslvrp isnsdikivy spqghfnaan laavnnaviq
      181 qrgnvkiqgv pngstairts phtpattpgl saihnlanva sqqtpliisq pakprivvdp
      241 kdpkaafkpq ilikqeppaa svgqeflrcs spaagviqas kpgpqpvsvv tqvvsspsil
      301 ppgvqivnvn pgrpgaptvv gqktlaprvv igspvrlapq mlaarpgaps vqntitlqpg
      361 mmrgtllvkt engqlqvvnv apslppnsta atmsgaptyr lqsvqncrhq tplvrrapih
      421 ehmkqawphl pnlgtqpvqq lqgrktnatp mvgtvlqqgq psinivptvp sstpiqtlgq
      481 plpqqtvtvk vaattttasv tqplsiqttv ansqtaattp sqmspntakk kcknflstli
      541 rlasdqpeqv asnvkgliqg lidgtiqped ftnqlqkeln sspqpclvpf lkkslpylrh
      601 slmtkeltie gvrpppqgav tlpstpaiip niqqvtgvar ptmphgttqv rvmtpttggl
      661 aaqlastagi qnvlaqhqrl vtprpattvv apgtksllva ktsnltaals patvgalqtk
      721 vatpvatrtp vtalkekrty sslrddddin dvaamggvnl veesqrilas naeivgtqir
      781 sckdenflft nplqrritsi avkygieeip sdvvalisha tqerlktlvq klgvvaehrl
      841 enlktdsrye atqdvkaqlk fleeldrlek krheeqerei llraaksrsk medpeqlklk
      901 qkakemqkae meemrqrean mtallaigpr kklktensgn sgaglgagsg ltnsfsklpm
      961 rprvkrvnlk dilflmeqer dsihstllyk sylk
//
LOCUS       XP_077503138             979 aa            linear   INV 25-NOV-2025
DEFINITION  transcription initiation factor TFIID subunit 4B-like isoform X4
            [Amblyomma americanum].
ACCESSION   XP_077503138
VERSION     XP_077503138.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647012.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..979
                     /product="transcription initiation factor TFIID subunit
                     4B-like isoform X4"
                     /calculated_mol_wt=104117
     Region          201..>459
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          514..601
                     /region_name="TAFH"
                     /note="NHR1 homology to TAF; pfam07531"
                     /db_xref="CDD:462195"
     Region          734..976
                     /region_name="TAF4"
                     /note="Transcription initiation factor TFIID component
                     TAF4 family; pfam05236"
                     /db_xref="CDD:461598"
     CDS             1..979
                     /gene="LOC144113727"
                     /coded_by="XM_077647012.1:31..2970"
                     /db_xref="GeneID:144113727"
ORIGIN      
        1 masaksleem lttdvdesvv selvgslesq lsssgihapn qeltvssatt nhvnsvpvsd
       61 glqrlphdgq khgvaaalan scpsivvsnf gktiattshi vgvsscassp asstntvmvt
      121 lpgalpasgy inqvtgtaqq allntslvrp isnsdikivy spqghfnaan laavnnaviq
      181 qrgnvkiqgv pngstairts phtpattpgl saihnlanva sqqtpliisq pakprivvdp
      241 kdpkaafkpq ilikqeppaa svgqeflrcs spaagviqas kpgpqpvsvv tqvvsspsil
      301 ppgvqivnvn pgrpgaptvv gqktlaprvv igspvrlapq mlaarpgaps vqryryhrpp
      361 ehdntitlqp gmmrgtllvk tengqlqvvn vapslppnst aatmsgapty rlqsvqnlgt
      421 qpvqqlqgrk tnatpmvgtv lqqgqpsini vptvpsstpi qtlgqplpqq tvtvkvaatt
      481 ttasvtqpls iqttvansqt aattpsqmsp ntakkkcknf lstlirlasd qpeqvasnvk
      541 gliqglidgt iqpedftnql qkelnsspqp clvpflkksl pylrhslmtk eltiegvrpp
      601 pqgavtlpst paiipniqqv tgvarptmph gttqvrvmtp ttgglaaqla stagiqnvla
      661 qhqrlvtprp attvvapgtk sllvaktsnl taalspatvg alqtkvatpv atrtpvtalk
      721 ekrtysslrd dddindvaam ggvnlveesq rilasnaeiv gtqirsckde nflftnplqr
      781 ritsiavkyg ieeipsdvva lishatqerl ktlvqklgvv aehrlenlkt dsryeatqdv
      841 kaqlkfleel drlekkrhee qereillraa ksrskmedpe qlklkqkake mqkaemeemr
      901 qreanmtall aigprkklkt ensgnsgagl gagsgltnsf sklpmrprvk rvnlkdilfl
      961 meqerdsihs tllyksylk
//
LOCUS       XP_077503139             988 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113727 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077503139
VERSION     XP_077503139.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647013.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..988
                     /product="uncharacterized protein LOC144113727 isoform X5"
                     /calculated_mol_wt=105043
     Region          523..610
                     /region_name="TAFH"
                     /note="NHR1 homology to TAF; pfam07531"
                     /db_xref="CDD:462195"
     Region          743..985
                     /region_name="TAF4"
                     /note="Transcription initiation factor TFIID component
                     TAF4 family; pfam05236"
                     /db_xref="CDD:461598"
     CDS             1..988
                     /gene="LOC144113727"
                     /coded_by="XM_077647013.1:24..2990"
                     /db_xref="GeneID:144113727"
ORIGIN      
        1 masaksleem lttdvdesvv selvgslesq lsssgihapn qeltvssatt nhvnsvpvsd
       61 glqrlphdgq khgvaaalan scpsivvsnf gktiattshi vgvsscassp asstntvmvt
      121 lpgalpasgy inqvtgtaqq allntslvrp isnsdikivy spqghfnaan laavnnaviq
      181 qrgnvkiqgv pngstairts phtpattpgl saihnlanva sqqtpliisq pakprivvdp
      241 kdpkaafkpq ilikqeppaa svgqeflrcs spaagviqas kpgpqpvsvv tqvvsspsil
      301 ppgvqivnvn pgrpgaptvv gqktlaprvv igspvrlapq mlaarpgaps vqntitlqpg
      361 mmrgtllvkt engqlqvvnv apslppnsta atmsgaptyr lqsvqncrhq tplvrrapih
      421 ehmkqnlgtq pvqqlqgrkt natpmvgtvl qqgqpsiniv ptvpsstpiq tlgqplpqqt
      481 vtvkvaattt tasvtqplsi qttvansqta attpsqmspn takkkcknfl stlirlasdq
      541 peqvasnvkg liqglidgti qpedftnqlq kelnsspqpc lvpflkkslp ylrhslmtke
      601 ltiegvrppp qgavtlpstp aiipniqqvt gvarptmphg ttqvrvmtpt tgglaaqlas
      661 tagiqnvlaq hqrlvtprpa ttvvapgtks llvaktsnlt aalspatvga lqtkvatpva
      721 trtpvtalke krtysslrdd ddindvaamg gvnlveesqr ilasnaeivg tqirsckden
      781 flftnplqrr itsiavkygi eeipsdvval ishatqerlk tlvqklgvva ehrlenlktd
      841 sryeatqdvk aqlkfleeld rlekkrheeq ereillraak srskmedpeq lklkqkakem
      901 qkaemeemrq reanmtalla igprkklkte nsgnsgaglg agsgltnsfs klpmrprvkr
      961 vnlkdilflm eqerdsihst llyksylk
//
LOCUS       XP_077503140             968 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113727 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077503140
VERSION     XP_077503140.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647014.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..968
                     /product="uncharacterized protein LOC144113727 isoform X6"
                     /calculated_mol_wt=102609
     Region          201..>448
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          503..590
                     /region_name="TAFH"
                     /note="NHR1 homology to TAF; pfam07531"
                     /db_xref="CDD:462195"
     Region          723..965
                     /region_name="TAF4"
                     /note="Transcription initiation factor TFIID component
                     TAF4 family; pfam05236"
                     /db_xref="CDD:461598"
     CDS             1..968
                     /gene="LOC144113727"
                     /coded_by="XM_077647014.1:24..2930"
                     /db_xref="GeneID:144113727"
ORIGIN      
        1 masaksleem lttdvdesvv selvgslesq lsssgihapn qeltvssatt nhvnsvpvsd
       61 glqrlphdgq khgvaaalan scpsivvsnf gktiattshi vgvsscassp asstntvmvt
      121 lpgalpasgy inqvtgtaqq allntslvrp isnsdikivy spqghfnaan laavnnaviq
      181 qrgnvkiqgv pngstairts phtpattpgl saihnlanva sqqtpliisq pakprivvdp
      241 kdpkaafkpq ilikqeppaa svgqeflrcs spaagviqas kpgpqpvsvv tqvvsspsil
      301 ppgvqivnvn pgrpgaptvv gqktlaprvv igspvrlapq mlaarpgaps vqntitlqpg
      361 mmrgtllvkt engqlqvvnv apslppnsta atmsgaptyr lqsvqnlgtq pvqqlqgrkt
      421 natpmvgtvl qqgqpsiniv ptvpsstpiq tlgqplpqqt vtvkvaattt tasvtqplsi
      481 qttvansqta attpsqmspn takkkcknfl stlirlasdq peqvasnvkg liqglidgti
      541 qpedftnqlq kelnsspqpc lvpflkkslp ylrhslmtke ltiegvrppp qgavtlpstp
      601 aiipniqqvt gvarptmphg ttqvrvmtpt tgglaaqlas tagiqnvlaq hqrlvtprpa
      661 ttvvapgtks llvaktsnlt aalspatvga lqtkvatpva trtpvtalke krtysslrdd
      721 ddindvaamg gvnlveesqr ilasnaeivg tqirsckden flftnplqrr itsiavkygi
      781 eeipsdvval ishatqerlk tlvqklgvva ehrlenlktd sryeatqdvk aqlkfleeld
      841 rlekkrheeq ereillraak srskmedpeq lklkqkakem qkaemeemrq reanmtalla
      901 igprkklkte nsgnsgaglg agsgltnsfs klpmrprvkr vnlkdilflm eqerdsihst
      961 llyksylk
//
LOCUS       XP_077503141             626 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113728 [Amblyomma americanum].
ACCESSION   XP_077503141
VERSION     XP_077503141.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647015.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..626
                     /product="uncharacterized protein LOC144113728"
                     /calculated_mol_wt=66045
     Site            order(147..149,151,171,177,190,192..193,196..197,199..201,
                     203..204)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238039"
     Site            order(147,150,193,196..197,200)
                     /site_type="other"
                     /note="specific DNA base contacts [nucleotide binding]"
                     /db_xref="CDD:238039"
     Region          163..201
                     /region_name="Homeobox_KN"
                     /note="Homeobox KN domain; pfam05920"
                     /db_xref="CDD:428673"
     CDS             1..626
                     /gene="LOC144113728"
                     /coded_by="XM_077647015.1:290..2170"
                     /db_xref="GeneID:144113728"
ORIGIN      
        1 msygqfgysy passqllvsg gasssspsca epssgassts sgsgaggape spmaaeqpvc
       61 yepsrflypr latglavngl yppsyadqsg yvaalgtssa afypsldnra twgtipqaaa
      121 acypydpala ayspygdryg amdsaarrkn atrettntlk awlyehrknp yptkgekiml
      181 aiitkmtltq vstwfanarr rlkkenkmtw eprnknnesg ddkadggasd tdndcgdkvd
      241 adaprdikpe lqdteldark pvmeraeasc ldlqslaqhg ashgslptaa fssyssknis
      301 vansssnhsv hdtssddgsg pispriqrss lrhpnayhed rpsgnslviv dplegsrpki
      361 wslaqtatsd sppgmrrspg pplsdrygfq qrlvsegsag asaaasgyyr dlhtdpsfms
      421 ttqplhaplg hqsskqpaaw inghspmaps sasstghtdv lnltpegspt syvlgrqsmg
      481 cispqppqsa lssvgrrfal aeekeptavr heqclvkpeg nvyeptahdm avgdganfcr
      541 rqhqaqalms lqgsclteaa tsaalaasrl pvhssymapg dkiekvtgkv gsphlqtagl
      601 aagnsnpcms nstafkpvsk sdvksi
//
LOCUS       XP_077503142             530 aa            linear   INV 25-NOV-2025
DEFINITION  iroquois-class homeodomain protein mirror [Amblyomma americanum].
ACCESSION   XP_077503142
VERSION     XP_077503142.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647016.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..530
                     /product="iroquois-class homeodomain protein mirror"
                     /calculated_mol_wt=55087
     Site            order(136..138,140,160,166,179,181..182,185..186,188..190,
                     192..193)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238039"
     Site            order(136,139,182,185..186,189)
                     /site_type="other"
                     /note="specific DNA base contacts [nucleotide binding]"
                     /db_xref="CDD:238039"
     Region          152..190
                     /region_name="Homeobox_KN"
                     /note="Homeobox KN domain; pfam05920"
                     /db_xref="CDD:428673"
     CDS             1..530
                     /gene="mirr"
                     /coded_by="XM_077647016.1:304..1896"
                     /db_xref="GeneID:144113729"
ORIGIN      
        1 mpgqttqslh gsapaagtpc cesgrpvltd pitgqtvcsc qydaqllnyq rlaasglpin
       61 mygtaayagd qgflplgaae qsafyspsan gfdlkenlea wrnlpyaasm yypydsaaia
      121 gypfangygm dlngarrkna trettstlka wlnehrknpy ptkgekimla iitkmtltqv
      181 stwfanarrr lkkenkmtws prnrcddddd adgedappar hdskdgqgsa aasrqesnst
      241 sssevaaraa eelarrnhql maaaastagl tetrkrprde sddlvdvddd srssdltdlr
      301 rpsgashsse eprhglqqgg gnlsdaslsd sessartpaa spnghsdvss apkpriwsiv
      361 dtatsgspsh smlspgapsa aavaarmasl qrsarldylt pyhktsswya stlgslgggs
      421 fplstvysvp timspaagla pvsagspltd sttaaslsrl rtaaafsaaa dmvlpkpaaa
      481 pppppgpsga fnlsrlapar ssaaaiaaav astssspsts qasapqpsps
//
LOCUS       XP_077503143             613 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat domain family member sosondowah isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077503143
VERSION     XP_077503143.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647017.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..613
                     /product="ankyrin repeat domain family member sosondowah
                     isoform X1"
                     /calculated_mol_wt=65955
     CDS             1..613
                     /gene="sowah"
                     /coded_by="XM_077647017.1:88..1929"
                     /db_xref="GeneID:144113730"
ORIGIN      
        1 mccsaaspak hlaaatrsai lrlfsgakgt ysvhggpapk aprigptpha ettarslppi
       61 aascisedes fgsldrllia sreqrgtgkr salshllnat ryrynstkvp ltgtgasaqa
      121 tpdipapvaa savrdclpas nmccsaaspa kqlaaatrsa ilrlfsgakg tysvhggpap
      181 kaprigptph aettarslpp iaasciseae sfgsrerlli asreqrgtgk rsalshllna
      241 tryrynstkv pltgtgasaq atpdipapva asavrdclpa snmccsaasp akqraaatrs
      301 ailrlfsgak atwyhvrqrf kdfvnrlasv keengtkyli lrneyfeelq ssrreftvgh
      361 ngameetdss vgvmqqdhad yfapsqgqtl khlqwrqftq dypmigdecp plspvgasri
      421 rsspcllden cyedvppplp kkknsfggsp krpdmplppp rgrknhgrrg sqveervrqf
      481 svekelegnv spkektiqdv tvspgtvkek tqiinrmaes sgvpakpalp ssglgtitsk
      541 rregpgaraa pdddttsvnt ldprrrewll raaqtdyhsl vrllredpdl yrfedftsvc
      601 skhfdvfllr aap
//
LOCUS       XP_077503144             388 aa            linear   INV 25-NOV-2025
DEFINITION  ankyrin repeat domain family member sosondowah isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077503144
VERSION     XP_077503144.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647018.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..388
                     /product="ankyrin repeat domain family member sosondowah
                     isoform X2"
                     /calculated_mol_wt=43594
     Region          293..325
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          298..>364
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     CDS             1..388
                     /gene="sowah"
                     /coded_by="XM_077647018.1:91..1257"
                     /db_xref="GeneID:144113730"
ORIGIN      
        1 mtdieefnfe lvkqflldrg gkvtnpelvr nfrhylndpv hkdhvrqrfk dfvnrlasvk
       61 eengtkylil rneyfeelqs srreftvghn gameetdssv gvmqqdhady fapsqgqtlk
      121 hlqwrqftqd ypmigdecpp lspvgasrir sspclldenc yedvppplpk kknsfggspk
      181 rpdmplpppr grknhgrrgs qveervrqfs vekelegnvs pkektiqdvt vspgtvkekt
      241 qiinrmaess gvpakpalps sglgtitskr regpgaraap dddttsvntl dprrrewllr
      301 aaqtdyhslv rllredpdly rfevsiesit alhwaakygs aeivkliags yslnpnvksv
      361 sglhtfasgv hvqpvphsrv tsglwsrc
//
LOCUS       XP_077503145             488 aa            linear   INV 25-NOV-2025
DEFINITION  tyrosine aminotransferase [Amblyomma americanum].
ACCESSION   XP_077503145
VERSION     XP_077503145.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647019.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (DAPXPI010026833.1) annotated
            using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 192 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..488
                     /product="tyrosine aminotransferase"
                     /calculated_mol_wt=52994
     Region          60..461
                     /region_name="tyr_amTase_E"
                     /note="tyrosine aminotransferase, eukaryotic; TIGR01264"
                     /db_xref="CDD:273529"
     CDS             1..488
                     /gene="Tat"
                     /coded_by="XM_077647019.1:16..1482"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 192 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144113733"
ORIGIN      
        1 mlsceplprs taaaeeafgl qsntcslala rtgvvassga smlpdtncnk kvgmrvrnsw
       61 nappsrfats tnnpirgfve daglvpnaek tlislsigdp tvfgnlvpce eilapietsl
      121 rslknhgyip stgtlaakea vaeyssthgl kvnpqdvilt cgcsqalemc vtvlansgqn
      181 iliprpgfsv ykthaesvgi kvkfynllpe rgwavdlvhl esqidantaa iivnnpsnpc
      241 gsvyskehlk dilavaarny vpiiadeiye hfvfqgqeyh pmgslsedvp ilscsgltkr
      301 flvpgwrtgw ivihdrhdvf argvkqglqs lsqkimggna liqgalpail kdtpqkfyde
      361 tvsvvqknae iaykalstip gmrpimpqga mylmvgiein kfpefdsdae fvkalfaees
      421 vfclpgrcfh ypnyfrivlt vpqemmleai drmaafckah yidenrlvqq sesslvigga
      481 piamearg
//
LOCUS       XP_077503146             189 aa            linear   INV 25-NOV-2025
DEFINITION  ER membrane protein complex subunit 4 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503146
VERSION     XP_077503146.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647020.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..189
                     /product="ER membrane protein complex subunit 4 isoform
                     X1"
                     /calculated_mol_wt=20875
     Region          48..161
                     /region_name="DUF1077"
                     /note="Protein of unknown function (DUF1077); pfam06417"
                     /db_xref="CDD:461902"
     CDS             1..189
                     /gene="EMC4"
                     /coded_by="XM_077647020.1:88..657"
                     /db_xref="GeneID:144113734"
ORIGIN      
        1 maaslgsraa akrhkwsfas sqrrtdkvte lasppgysss lgqghseasr eteanlivkk
       61 swdvalapwk qvpmnlfimy magnsisifp immvgmlflr pvkalltiqs tfkmieggqa
      121 ilqkivylfg nlaclalaly kcssmgllpt hasdwldfve plqacahdcf eahiirdnsl
      181 hflfvfmmq
//
LOCUS       XP_077503147             175 aa            linear   INV 25-NOV-2025
DEFINITION  ER membrane protein complex subunit 4 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503147
VERSION     XP_077503147.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647021.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..175
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..175
                     /product="ER membrane protein complex subunit 4 isoform
                     X2"
                     /calculated_mol_wt=19016
     Region          48..161
                     /region_name="DUF1077"
                     /note="Protein of unknown function (DUF1077); pfam06417"
                     /db_xref="CDD:461902"
     CDS             1..175
                     /gene="EMC4"
                     /coded_by="XM_077647021.1:88..615"
                     /db_xref="GeneID:144113734"
ORIGIN      
        1 maaslgsraa akrhkwsfas sqrrtdkvte lasppgysss lgqghseasr eteanlivkk
       61 swdvalapwk qvpmnlfimy magnsisifp immvgmlflr pvkalltiqs tfkmieggqa
      121 ilqkivylfg nlaclalaly kcssmgllpt hasdwldfve plqriehsgg giils
//
LOCUS       XP_077503148             592 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylinositol-3-phosphatase SAC1 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503148
VERSION     XP_077503148.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647022.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..592
                     /product="phosphatidylinositol-3-phosphatase SAC1 isoform
                     X1"
                     /calculated_mol_wt=67914
     Region          7..561
                     /region_name="COG5329"
                     /note="Phosphoinositide polyphosphatase (Sac family)
                     [Signal transduction mechanisms]"
                     /db_xref="CDD:227637"
     CDS             1..592
                     /gene="Sac1"
                     /coded_by="XM_077647022.1:172..1950"
                     /db_xref="GeneID:144113735"
ORIGIN      
        1 mmsevygecv lystperyii epsnssdksl vidrvsreit lsakpdipph avrrviygiv
       61 giihllagpy liviverrrv gdingqviwr ikateaysyt rtslhlteqq nqynkqytam
      121 vqsvlstpnf yysttydlsh slqklynttp dflqmglmer adqrfvwnhy lmsefsnqve
      181 lrkfclpiih gfvyikacai nghgftfali srrscyragt rmfmrgldse ghaanfvete
      241 qiiegdtars sfiqtrgsip lfwtqlpnlr ykpapalssg lnhleafqkh fdnqiytygs
      301 qviislidqk gpekalgrql qevvnlannp kikyeafdfh hecrkmqwdr lsilmdriaq
      361 dqdafgyfmm lydgsvpclq tgvfrtncid cldrtnvvqs llarrsleqq lqrtgilmpg
      421 etikqhpvfe tlyknvwadn gdfcsiqyag tgalktdytr tgkrtilgal rdgynsavry
      481 iknnfndgfr qdaidlflgn yrvqegegaa lscpltvrre kkywvlpgfl llaiaccflc
      541 lfsssvysme tfmyflfwvt mtvltlmaiv yygtefvdfp klrdlapnrr sd
//
LOCUS       XP_077503149             596 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylinositol-3-phosphatase SAC1 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503149
VERSION     XP_077503149.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647023.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..596
                     /product="phosphatidylinositol-3-phosphatase SAC1 isoform
                     X2"
                     /calculated_mol_wt=68150
     Region          60..565
                     /region_name="COG5329"
                     /note="Phosphoinositide polyphosphatase (Sac family)
                     [Signal transduction mechanisms]"
                     /db_xref="CDD:227637"
     CDS             1..596
                     /gene="Sac1"
                     /coded_by="XM_077647023.1:84..1874"
                     /db_xref="GeneID:144113735"
ORIGIN      
        1 maaatdggvy dslilystpe ryiiepsnss dkslvidrvs reitlsakpd ipphavrrvi
       61 ygivgiihll agpylivive rrrvgdingq viwrikatea ysytrtslhl teqqnqynkq
      121 ytamvqsvls tpnfyystty dlshslqkly nttpdflqmg lmeradqrfv wnhylmsefs
      181 nqvelrkfcl piihgfvyik acainghgft falisrrscy ragtrmfmrg ldseghaanf
      241 veteqiiegd tarssfiqtr gsiplfwtql pnlrykpapa lssglnhlea fqkhfdnqiy
      301 tygsqviisl idqkgpekal grqlqevvnl annpkikyea fdfhhecrkm qwdrlsilmd
      361 riaqdqdafg yfmmlydgsv pclqtgvfrt ncidcldrtn vvqsllarrs leqqlqrtgi
      421 lmpgetikqh pvfetlyknv wadngdfcsi qyagtgalkt dytrtgkrti lgalrdgyns
      481 avryiknnfn dgfrqdaidl flgnyrvqeg egaalscplt vrrekkywvl pgflllaiac
      541 cflclfsssv ysmetfmyfl fwvtmtvltl maivyygtef vdfpklrdla pnrrsd
//
LOCUS       XP_077503150             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113736 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503150
VERSION     XP_077503150.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647024.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144113736 isoform X1"
                     /calculated_mol_wt=14224
     CDS             1..133
                     /gene="LOC144113736"
                     /coded_by="XM_077647024.1:175..576"
                     /db_xref="GeneID:144113736"
ORIGIN      
        1 mdrligrvtf vtaaspalat cdeaeavsgl qqckdqihqi dkntfnasna dhkeqlccsa
       61 vaqfedcirt airhagchhe vdflvtllms kardmlghiy dancwydckk avasmlsasa
      121 glvalalval lgt
//
LOCUS       XP_077503151             132 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113736 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503151
VERSION     XP_077503151.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647025.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..132
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..132
                     /product="uncharacterized protein LOC144113736 isoform X2"
                     /calculated_mol_wt=14137
     CDS             1..132
                     /gene="LOC144113736"
                     /coded_by="XM_077647025.1:145..543"
                     /db_xref="GeneID:144113736"
ORIGIN      
        1 mdrligrvtf vtaaspalat cdeaeavsgl qqckdqihqi dkntfnasna dhkeqlccav
       61 aqfedcirta irhagchhev dflvtllmsk ardmlghiyd ancwydckka vasmlsasag
      121 lvalalvall gt
//
LOCUS       XP_077503152             264 aa            linear   INV 25-NOV-2025
DEFINITION  proteasome alpha3 subunit [Amblyomma americanum].
ACCESSION   XP_077503152
VERSION     XP_077503152.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647026.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..264
                     /product="proteasome alpha3 subunit"
                     /calculated_mol_wt=29850
     Region          3..216
                     /region_name="proteasome_alpha_type_4"
                     /note="The 20S proteasome, multisubunit proteolytic
                     complex, is the central enzyme of nonlysosomal protein
                     degradation in both the cytosol and nucleus. It is
                     composed of 28 subunits arranged as four homoheptameric
                     rings that stack on top of one another forming...;
                     cd03752"
                     /db_xref="CDD:239721"
     Site            order(7..10,12..13,15..16,20,23,26..27,30,38,53,55..56,
                     80..82,84..85,116,119,122..123,126..130,148,153..154,156,
                     158..159,161)
                     /site_type="other"
                     /note="alpha subunit interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:239721"
     Site            order(32,48,50,64,166)
                     /site_type="active"
                     /db_xref="CDD:239721"
     CDS             1..264
                     /gene="Prosalpha3"
                     /coded_by="XM_077647026.1:142..936"
                     /db_xref="GeneID:144113737"
ORIGIN      
        1 marrydsrtt ifspegrlyq veyameaigh agtclgilas dgillaaerr ntnklldevf
       61 taekiyklhd dmacsvagit sdanvltnel rqiaqryllq ygesipcerl vswlcdlkqa
      121 ytqyggkrpf gvsilymgwd khygyqlyqs dpsgnysgwk atcignnsaa avsilkqeyk
      181 enettlkdal alaikvlskt ldmtkltsdk lematltrdm krnktrvnil hqseveklik
      241 kheeeeakle atkkekerek qsrs
//
LOCUS       XP_077503153             569 aa            linear   INV 25-NOV-2025
DEFINITION  malonate--CoA ligase ACSF3, mitochondrial-like [Amblyomma
            americanum].
ACCESSION   XP_077503153
VERSION     XP_077503153.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647027.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..569
                     /product="malonate--CoA ligase ACSF3, mitochondrial-like"
                     /calculated_mol_wt=63120
     Region          44..563
                     /region_name="MCS"
                     /note="Malonyl-CoA synthetase (MCS); cd05941"
                     /db_xref="CDD:341264"
     Site            order(185,188..193,195..196)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341264"
     Site            order(188,228..229,232..234,281,283,287,325..327,346..351,
                     355,370,448,459,467,469,471,474,503,540)
                     /site_type="active"
                     /db_xref="CDD:341264"
     Site            order(188,325..327,346..351,370,448,459,467,469,474)
                     /site_type="other"
                     /note="AMP binding site [chemical binding]"
                     /db_xref="CDD:341264"
     Site            order(228..229,232..234,281,283,287,325,347,349..351,355,
                     469,471..473,503,540)
                     /site_type="other"
                     /note="CoA binding site [chemical binding]"
                     /db_xref="CDD:341264"
     CDS             1..569
                     /gene="LOC144113738"
                     /coded_by="XM_077647027.1:203..1912"
                     /db_xref="GeneID:144113738"
ORIGIN      
        1 mtpvlflakl ravalqhvrv srrflgsfpp pfesifnkaa yyqdrlalvd aegtytfsnl
       61 lassstlqvh iddllrgssr qrisflcpnt vsyvitqwac wmsgnvavpl ywnhprsvle
      121 yyikdsqsal lvstkayadr lqpiakeldl plvvldedvk ketgftpskq ktdewkrlkh
      181 kdaqilytsg ttgppkgvvt thnnlfvqas aiigawefss sdvvlhtlpl hhthgvvnal
      241 vaplyagstc vmlpqfeaae vwnyliepps rgpriniyma vptiyakliq hydklfsgns
      301 apfsrskefi raacsqkirl mvsgsaslpl pvlerwkeit ghtllerygm teigmaltnp
      361 lhgtrkpgyv gkpfpgvkvc iakpnayaaa gydvistgde ygtkvvkgse netgdlhvkg
      421 pnvfkeywnk peatkeafts dgwfktgdta eykdgayril grtssdiiks ggykisaldv
      481 erhllahpdi rdcvvvglpd itwgekvaca valrdtvael tlqdlrewsr srmeaamipv
      541 llrivqeipr namgkvnkrd ivaqlfanc
//
LOCUS       XP_077503154             387 aa            linear   INV 25-NOV-2025
DEFINITION  divergent protein kinase domain 1C [Amblyomma americanum].
ACCESSION   XP_077503154
VERSION     XP_077503154.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647028.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..387
                     /product="divergent protein kinase domain 1C"
                     /calculated_mol_wt=42882
     Region          8..140
                     /region_name="PIP49_N"
                     /note="N-term cysteine-rich ER, FAM69; pfam14875"
                     /db_xref="CDD:464353"
     Region          166..357
                     /region_name="PIP49_C"
                     /note="Protein-kinase domain of FAM69; pfam12260"
                     /db_xref="CDD:463512"
     CDS             1..387
                     /gene="aln"
                     /coded_by="XM_077647028.1:141..1304"
                     /db_xref="GeneID:144113739"
ORIGIN      
        1 mkalqcafrp rrsakpvkvl gallsigvlv flaqlsfpgc tnadietflv nicdayrkge
       61 isgnlcthlc srdisdiqcq sqhigkdivf taiwnskpvv vkakkrlits ldpvywvdnn
      121 klnhypdnat ftrmvnsqis yvfrrnstwv etfygkqfsi aemnsiyall qqteylftla
      181 leglnvapki lgtcghayav eflspletrf ssghrdatas sfkdramial rilntldile
      241 nameeeihqc dmksnhfgvd akglvkmldl dafglhstvq aniassghcd vdkdcdffdc
      301 agrcnslgqc davvlnnnlq ricrnvflgn lfgrytgllr sppsdiatel hallnecgsm
      361 sitplsrnhs tviktkfvel lhsvlvp
//
LOCUS       XP_077503155             148 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113741 [Amblyomma americanum].
ACCESSION   XP_077503155
VERSION     XP_077503155.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647029.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="uncharacterized protein LOC144113741"
                     /calculated_mol_wt=17365
     CDS             1..148
                     /gene="LOC144113741"
                     /coded_by="XM_077647029.1:217..663"
                     /db_xref="GeneID:144113741"
ORIGIN      
        1 mglhavtvll iallvvvaes rreikykvkk qdflhryrel sknrteenvy dichkvalrr
       61 cgqnfmrafn lmeylsgady yntqctlrka ffacldklrq kpcrkrrasg lddkrfrkkl
      121 adalwetrvc vlgikdieel ppkkknlk
//
LOCUS       XP_077503156            1188 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113742 [Amblyomma americanum].
ACCESSION   XP_077503156
VERSION     XP_077503156.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647030.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1188
                     /product="uncharacterized protein LOC144113742"
                     /calculated_mol_wt=133296
     Region          140..465
                     /region_name="Fanconi_A_N"
                     /note="Fanconi anaemia group A protein N terminus;
                     pfam15865"
                     /db_xref="CDD:464911"
     CDS             1..1188
                     /gene="LOC144113742"
                     /coded_by="XM_077647030.1:157..3723"
                     /db_xref="GeneID:144113742"
ORIGIN      
        1 metnasfide letlitgsag areilcelsd psivlkgdrk dldyvetddl lvmqaallsc
       61 tnrrddvslt kenfsdlgya qiaihcaesd dessgqncll ndqgrkaalt ltahvnatst
      121 skgipvnfak vlasctvcpl ellcrlhagg vmfiedyial nvqkpqviad lsrslfaccq
      181 nceqnepfaf tfsvfmcalv ssaysasdhn iaspsvrkvc ctvletvlqr llkaneegqt
      241 ltcgdllgpl lkrplvqhsa ircyaqells cilaynpvyk vaqamsshhm wtykkcspwl
      301 fgmfqkamqv ltvsdvtsql sktlnskevn wymlltalsv yvttyhdckl liglietllk
      361 tafkcldmey lvsaillarh aaqeapqifp pysswfekyf gfsdttcans srtfaflvkf
      421 lsdmvpfeqa kylkvhlqkp ayassecrml yhdyvalakt rlhdleavde rntscedqeh
      481 saqvhesvqk aiedfaakgk lsrsvieasi fkkpyftgqf lpvlldpknf sderevvrsm
      541 aqkllqhgki srerydkfih eceeqhgdeg dvemensvfm pehllaqlrs gckdcetiil
      601 rlieevkkne reypnrtlel gheipnskei fevlqfasea ychqppqawi ksvllamgss
      661 lctvhllaaf lwnqlttqmd kqaaevfsyf llyvpalwed qllkvsvhgt tymsmheavf
      721 sclslstsqg rmqflqlsvt ylteaakqvp stslppcvpk vlvkkfqfvm qraeskldki
      781 dsdtwkkcsk spiedwiqsg klsvsewvnf esqitddvss tspvlrqdyl qfladadsar
      841 clcltllrvl vsgpgvpnys hlqillqsll lnppsgvfdk avpfatealt qmeaeiasss
      901 vdeaemavnf fkmychlpay cllvnrrddc ptsesvkifi rvvnkylvlc sqeetpglal
      961 hlfkglfqsn lskshassil eqcpilvlyl irhwkivspv laleyikcee lsslltgikk
     1021 lytlaeefia gseesgkrls kitqtwfpaf vlacslsahq vvpphyktlk palqtmaiqf
     1081 llllckrdhn nhndglkvya ssfleenpnl cfslepasfn talmqnlmsa ldfflllvfc
     1141 fslvvenver qepkrsccmn talllmthid tidedvlnvg mssahlqg
//
LOCUS       XP_077503157             319 aa            linear   INV 25-NOV-2025
DEFINITION  exosome complex exonuclease RRP42-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503157
VERSION     XP_077503157.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647031.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..319
                     /product="exosome complex exonuclease RRP42-like isoform
                     X1"
                     /calculated_mol_wt=34156
     Region          5..285
                     /region_name="RNase_PH_RRP42"
                     /note="RRP42 subunit of eukaryotic exosome; cd11367"
                     /db_xref="CDD:206772"
     Site            order(5..10,12..13,16..17,20,22,24..27,34,55,244)
                     /site_type="other"
                     /note="Rrp4 interface [polypeptide binding]"
                     /db_xref="CDD:206772"
     Site            order(43..45,50,57,61,63,82,87..88,103,111,136,138,
                     169..170,257,261..274,279..280,283..284)
                     /site_type="other"
                     /note="hexamer interface [polypeptide binding]"
                     /db_xref="CDD:206772"
     CDS             1..319
                     /gene="LOC144113743"
                     /coded_by="XM_077647031.1:1526..2485"
                     /db_xref="GeneID:144113743"
ORIGIN      
        1 magvliseae ktyivhgiqe dlrcdgrscl eyrfleletg ivsncsgssh vrlantdilv
       61 gikaeldapd piargqgrie ffvdctanad pefegrggek vaskirsala etfgspscld
      121 lkslavipgq qvwvlyvdvl vthpwhlaty alqlcsfpvp svfkrekile cggslldavs
      181 vgvkaalfdl kipkvsvtid engeaeidvs ddpydvskls vanvpcfitl skvgqqfvld
      241 atqeeeacav gslamavtag gkvaslfkgg igslhpenvm diieatpcmc traswcrftn
      301 vmkrcsiflr qvfavtaca
//
LOCUS       XP_077503158             316 aa            linear   INV 25-NOV-2025
DEFINITION  exosome complex component RRP42-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503158
VERSION     XP_077503158.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647032.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..316
                     /product="exosome complex component RRP42-like isoform X2"
                     /calculated_mol_wt=33560
     Region          5..302
                     /region_name="RNase_PH_RRP42"
                     /note="RRP42 subunit of eukaryotic exosome; cd11367"
                     /db_xref="CDD:206772"
     Site            order(5..10,12..13,16..17,20,22,24..27,34,55,244)
                     /site_type="other"
                     /note="Rrp4 interface [polypeptide binding]"
                     /db_xref="CDD:206772"
     Site            order(43..45,50,57,61,63,82,87..88,103,111,136,138,
                     169..170,257,261..274,279..280,283..284,286..287)
                     /site_type="other"
                     /note="hexamer interface [polypeptide binding]"
                     /db_xref="CDD:206772"
     CDS             1..316
                     /gene="LOC144113743"
                     /coded_by="XM_077647032.1:1526..2476"
                     /db_xref="GeneID:144113743"
ORIGIN      
        1 magvliseae ktyivhgiqe dlrcdgrscl eyrfleletg ivsncsgssh vrlantdilv
       61 gikaeldapd piargqgrie ffvdctanad pefegrggek vaskirsala etfgspscld
      121 lkslavipgq qvwvlyvdvl vthpwhlaty alqlcsfpvp svfkrekile cggslldavs
      181 vgvkaalfdl kipkvsvtid engeaeidvs ddpydvskls vanvpcfitl skvgqqfvld
      241 atqeeeacav gslamavtag gkvaslfkgg igslhpenvm diievgkdig ialnsavmkn
      301 lelessskge kkgfll
//
LOCUS       XP_077503159             292 aa            linear   INV 25-NOV-2025
DEFINITION  exosome complex exonuclease RRP42-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503159
VERSION     XP_077503159.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647033.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..292
                     /product="exosome complex exonuclease RRP42-like isoform
                     X3"
                     /calculated_mol_wt=31019
     Region          5..258
                     /region_name="RNase_PH_RRP42"
                     /note="RRP42 subunit of eukaryotic exosome; cd11367"
                     /db_xref="CDD:206772"
     Site            order(5..10,12..13,16..17,20,22,24..27,34,55,217)
                     /site_type="other"
                     /note="Rrp4 interface [polypeptide binding]"
                     /db_xref="CDD:206772"
     Site            order(43..45,50,57,61,63,82,87..88,103,111,136,138,
                     142..143,230,234..247,252..253,256..257)
                     /site_type="other"
                     /note="hexamer interface [polypeptide binding]"
                     /db_xref="CDD:206772"
     CDS             1..292
                     /gene="LOC144113743"
                     /coded_by="XM_077647033.1:1526..2404"
                     /db_xref="GeneID:144113743"
ORIGIN      
        1 magvliseae ktyivhgiqe dlrcdgrscl eyrfleletg ivsncsgssh vrlantdilv
       61 gikaeldapd piargqgrie ffvdctanad pefegrggek vaskirsala etfgspscld
      121 lkslavipgq qvwvlyvdvl ilecggslld avsvgvkaal fdlkipkvsv tidengeaei
      181 dvsddpydvs klsvanvpcf itlskvgqqf vldatqeeea cavgslamav taggkvaslf
      241 kggigslhpe nvmdiieatp cmctraswcr ftnvmkrcsi flrqvfavta ca
//
LOCUS       XP_077503160             289 aa            linear   INV 25-NOV-2025
DEFINITION  exosome complex component RRP42-like isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503160
VERSION     XP_077503160.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647034.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..289
                     /product="exosome complex component RRP42-like isoform X4"
                     /calculated_mol_wt=30422
     Region          5..275
                     /region_name="RNase_PH_RRP42"
                     /note="RRP42 subunit of eukaryotic exosome; cd11367"
                     /db_xref="CDD:206772"
     Site            order(5..10,12..13,16..17,20,22,24..27,34,55,217)
                     /site_type="other"
                     /note="Rrp4 interface [polypeptide binding]"
                     /db_xref="CDD:206772"
     Site            order(43..45,50,57,61,63,82,87..88,103,111,136,138,
                     142..143,230,234..247,252..253,256..257,259..260)
                     /site_type="other"
                     /note="hexamer interface [polypeptide binding]"
                     /db_xref="CDD:206772"
     CDS             1..289
                     /gene="LOC144113743"
                     /coded_by="XM_077647034.1:1526..2395"
                     /db_xref="GeneID:144113743"
ORIGIN      
        1 magvliseae ktyivhgiqe dlrcdgrscl eyrfleletg ivsncsgssh vrlantdilv
       61 gikaeldapd piargqgrie ffvdctanad pefegrggek vaskirsala etfgspscld
      121 lkslavipgq qvwvlyvdvl ilecggslld avsvgvkaal fdlkipkvsv tidengeaei
      181 dvsddpydvs klsvanvpcf itlskvgqqf vldatqeeea cavgslamav taggkvaslf
      241 kggigslhpe nvmdiievgk digialnsav mknlelesss kgekkgfll
//
LOCUS       XP_077503161             264 aa            linear   INV 25-NOV-2025
DEFINITION  exosome complex exonuclease RRP42-like isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077503161
VERSION     XP_077503161.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647035.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..264
                     /product="exosome complex exonuclease RRP42-like isoform
                     X5"
                     /calculated_mol_wt=28288
     Region          5..>235
                     /region_name="RNase_PH_RRP42"
                     /note="RRP42 subunit of eukaryotic exosome; cd11367"
                     /db_xref="CDD:206772"
     Site            order(5..10,12..13,16..17,20,22,24..27,34,55)
                     /site_type="other"
                     /note="Rrp4 interface [polypeptide binding]"
                     /db_xref="CDD:206772"
     CDS             1..264
                     /gene="LOC144113743"
                     /coded_by="XM_077647035.1:1526..2320"
                     /db_xref="GeneID:144113743"
ORIGIN      
        1 magvliseae ktyivhgiqe dlrcdgrscl eyrfleletg ivsncsgssh vrlantdilv
       61 gikaeldapd piargqgrie ffvdctanad pefegrggek vaskirsala etfgspscld
      121 lkslavipgq qvwvlyvdvl vthpwhlaty alqlcsfpvp svfkrekile cggslldavs
      181 vgvkaalfdl kipkvsvtid engeaeidvs ddpydvskls vanvpcfitl skvgkdigia
      241 lnsavmknle lessskgekk gfll
//
LOCUS       XP_077503162             259 aa            linear   INV 25-NOV-2025
DEFINITION  exosome complex exonuclease RRP42-like isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077503162
VERSION     XP_077503162.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647036.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..259
                     /product="exosome complex exonuclease RRP42-like isoform
                     X6"
                     /calculated_mol_wt=27993
     Region          5..>238
                     /region_name="RNase_PH_RRP42"
                     /note="RRP42 subunit of eukaryotic exosome; cd11367"
                     /db_xref="CDD:206772"
     Site            order(5..10,12..13,16..17,20,22,24..27,34,55)
                     /site_type="other"
                     /note="Rrp4 interface [polypeptide binding]"
                     /db_xref="CDD:206772"
     CDS             1..259
                     /gene="LOC144113743"
                     /coded_by="XM_077647036.1:1526..2305"
                     /db_xref="GeneID:144113743"
ORIGIN      
        1 magvliseae ktyivhgiqe dlrcdgrscl eyrfleletg ivsncsgssh vrlantdilv
       61 gikaeldapd piargqgrie ffvdctanad pefegrggek vaskirsala etfgspscld
      121 lkslavipgq qvwvlyvdvl vthpwhlaty alqlcsfpvp svfkrekile cggslldavs
      181 vgvkaalfdl kipkvsvtid engeaeidvs ddpydvskls vanvpcfitl skidaaclhl
      241 kysnnlknst fnlkkvmcl
//
LOCUS       XP_077503163             219 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113744 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503163
VERSION     XP_077503163.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647037.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..219
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..219
                     /product="uncharacterized protein LOC144113744 isoform X1"
                     /calculated_mol_wt=24589
     Region          56..215
                     /region_name="DUF4674"
                     /note="Domain of unknown function (DUF4674); pfam15719"
                     /db_xref="CDD:464823"
     CDS             1..219
                     /gene="LOC144113744"
                     /coded_by="XM_077647037.1:126..785"
                     /db_xref="GeneID:144113744"
ORIGIN      
        1 macsnyevst atlsflqkis kacnaislpv arclsyrlme csehrsigek gcptkcescg
       61 iawdpssvkm rtksrpvlrr nmrklfakeq hrpwlltskq kkqlksfrra ttmlvflcrv
      121 cgkkveqpcp kdlkkqsska ssntlfcnkp vsttskkkpi sspahvllkk rkeekcvvpd
      181 pqketasvkr nrsllkrvli eedtrktssg glhsflssl
//
LOCUS       XP_077503164             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113744 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503164
VERSION     XP_077503164.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647038.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144113744 isoform X2"
                     /calculated_mol_wt=20453
     Region          18..177
                     /region_name="DUF4674"
                     /note="Domain of unknown function (DUF4674); pfam15719"
                     /db_xref="CDD:464823"
     CDS             1..181
                     /gene="LOC144113744"
                     /coded_by="XM_077647038.1:32..577"
                     /db_xref="GeneID:144113744"
ORIGIN      
        1 mecsehrsig ekgcptkces cgiawdpssv kmrtksrpvl rrnmrklfak eqhrpwllts
       61 kqkkqlksfr rattmlvflc rvcgkkveqp cpkdlkkqss kassntlfcn kpvsttskkk
      121 pisspahvll kkrkeekcvv pdpqketasv krnrsllkrv lieedtrkts sgglhsflss
      181 l
//
LOCUS       XP_077503165             315 aa            linear   INV 25-NOV-2025
DEFINITION  carbonic anhydrase 1-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503165
VERSION     XP_077503165.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647039.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..315
                     /product="carbonic anhydrase 1-like isoform X1"
                     /calculated_mol_wt=35193
     Region          48..311
                     /region_name="Carb_anhydrase"
                     /note="Eukaryotic-type carbonic anhydrase; pfam00194"
                     /db_xref="CDD:459707"
     CDS             1..315
                     /gene="LOC144113745"
                     /coded_by="XM_077647039.1:415..1362"
                     /db_xref="GeneID:144113745"
ORIGIN      
        1 mpasrlwtlc snraltipav aikrfvshsa ptqaegfrhl rhrkvvicgp snwakrfpka
       61 agdnqspidi vrsdvqqdpf ltenplrwty tglqctslln tgsgwrvdvs apgprdakpi
      121 npirlnlrgg plhnnyqmvq fhshwgtcse tgsehtvdge hyagelhlvh ynvekynkps
      181 eaacsdkgls vvaiffkegk phhelkkitd cmskvtfkgm kcpleqdidi nsiipanssy
      241 wtyegslttp pcyesvtwiv fkepievsre qfeafrelms ykegggpqva tdgpilknfr
      301 ppqplngrvv repae
//
LOCUS       XP_077503166             315 aa            linear   INV 25-NOV-2025
DEFINITION  carbonic anhydrase 1-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503166
VERSION     XP_077503166.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647040.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..315
                     /product="carbonic anhydrase 1-like isoform X1"
                     /calculated_mol_wt=35193
     Region          48..311
                     /region_name="Carb_anhydrase"
                     /note="Eukaryotic-type carbonic anhydrase; pfam00194"
                     /db_xref="CDD:459707"
     CDS             1..315
                     /gene="LOC144113745"
                     /coded_by="XM_077647040.1:400..1347"
                     /db_xref="GeneID:144113745"
ORIGIN      
        1 mpasrlwtlc snraltipav aikrfvshsa ptqaegfrhl rhrkvvicgp snwakrfpka
       61 agdnqspidi vrsdvqqdpf ltenplrwty tglqctslln tgsgwrvdvs apgprdakpi
      121 npirlnlrgg plhnnyqmvq fhshwgtcse tgsehtvdge hyagelhlvh ynvekynkps
      181 eaacsdkgls vvaiffkegk phhelkkitd cmskvtfkgm kcpleqdidi nsiipanssy
      241 wtyegslttp pcyesvtwiv fkepievsre qfeafrelms ykegggpqva tdgpilknfr
      301 ppqplngrvv repae
//
LOCUS       XP_077503167             315 aa            linear   INV 25-NOV-2025
DEFINITION  carbonic anhydrase 1-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503167
VERSION     XP_077503167.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647041.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..315
                     /product="carbonic anhydrase 1-like isoform X1"
                     /calculated_mol_wt=35193
     Region          48..311
                     /region_name="Carb_anhydrase"
                     /note="Eukaryotic-type carbonic anhydrase; pfam00194"
                     /db_xref="CDD:459707"
     CDS             1..315
                     /gene="LOC144113745"
                     /coded_by="XM_077647041.1:142..1089"
                     /db_xref="GeneID:144113745"
ORIGIN      
        1 mpasrlwtlc snraltipav aikrfvshsa ptqaegfrhl rhrkvvicgp snwakrfpka
       61 agdnqspidi vrsdvqqdpf ltenplrwty tglqctslln tgsgwrvdvs apgprdakpi
      121 npirlnlrgg plhnnyqmvq fhshwgtcse tgsehtvdge hyagelhlvh ynvekynkps
      181 eaacsdkgls vvaiffkegk phhelkkitd cmskvtfkgm kcpleqdidi nsiipanssy
      241 wtyegslttp pcyesvtwiv fkepievsre qfeafrelms ykegggpqva tdgpilknfr
      301 ppqplngrvv repae
//
LOCUS       XP_077503168             315 aa            linear   INV 25-NOV-2025
DEFINITION  carbonic anhydrase 1-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503168
VERSION     XP_077503168.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647042.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..315
                     /product="carbonic anhydrase 1-like isoform X1"
                     /calculated_mol_wt=35193
     Region          48..311
                     /region_name="Carb_anhydrase"
                     /note="Eukaryotic-type carbonic anhydrase; pfam00194"
                     /db_xref="CDD:459707"
     CDS             1..315
                     /gene="LOC144113745"
                     /coded_by="XM_077647042.1:314..1261"
                     /db_xref="GeneID:144113745"
ORIGIN      
        1 mpasrlwtlc snraltipav aikrfvshsa ptqaegfrhl rhrkvvicgp snwakrfpka
       61 agdnqspidi vrsdvqqdpf ltenplrwty tglqctslln tgsgwrvdvs apgprdakpi
      121 npirlnlrgg plhnnyqmvq fhshwgtcse tgsehtvdge hyagelhlvh ynvekynkps
      181 eaacsdkgls vvaiffkegk phhelkkitd cmskvtfkgm kcpleqdidi nsiipanssy
      241 wtyegslttp pcyesvtwiv fkepievsre qfeafrelms ykegggpqva tdgpilknfr
      301 ppqplngrvv repae
//
LOCUS       XP_077503169             315 aa            linear   INV 25-NOV-2025
DEFINITION  carbonic anhydrase 1-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503169
VERSION     XP_077503169.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647043.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..315
                     /product="carbonic anhydrase 1-like isoform X1"
                     /calculated_mol_wt=35193
     Region          48..311
                     /region_name="Carb_anhydrase"
                     /note="Eukaryotic-type carbonic anhydrase; pfam00194"
                     /db_xref="CDD:459707"
     CDS             1..315
                     /gene="LOC144113745"
                     /coded_by="XM_077647043.1:88..1035"
                     /db_xref="GeneID:144113745"
ORIGIN      
        1 mpasrlwtlc snraltipav aikrfvshsa ptqaegfrhl rhrkvvicgp snwakrfpka
       61 agdnqspidi vrsdvqqdpf ltenplrwty tglqctslln tgsgwrvdvs apgprdakpi
      121 npirlnlrgg plhnnyqmvq fhshwgtcse tgsehtvdge hyagelhlvh ynvekynkps
      181 eaacsdkgls vvaiffkegk phhelkkitd cmskvtfkgm kcpleqdidi nsiipanssy
      241 wtyegslttp pcyesvtwiv fkepievsre qfeafrelms ykegggpqva tdgpilknfr
      301 ppqplngrvv repae
//
LOCUS       XP_077503170             304 aa            linear   INV 25-NOV-2025
DEFINITION  carbonic anhydrase 1-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503170
VERSION     XP_077503170.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647044.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..304
                     /product="carbonic anhydrase 1-like isoform X2"
                     /calculated_mol_wt=33919
     Region          48..300
                     /region_name="Carb_anhydrase"
                     /note="Eukaryotic-type carbonic anhydrase; pfam00194"
                     /db_xref="CDD:459707"
     CDS             1..304
                     /gene="LOC144113745"
                     /coded_by="XM_077647044.1:415..1329"
                     /db_xref="GeneID:144113745"
ORIGIN      
        1 mpasrlwtlc snraltipav aikrfvshsa ptqaegfrhl rhrkvvicgp snwakrfpka
       61 agdnqspidi vrsdvqqdpf ltenplrwty tglqctslln tgsgwrvdvs apgpnlrggp
      121 lhnnyqmvqf hshwgtcset gsehtvdgeh yagelhlvhy nvekynkpse aacsdkglsv
      181 vaiffkegkp hhelkkitdc mskvtfkgmk cpleqdidin siipanssyw tyegslttpp
      241 cyesvtwivf kepievsreq feafrelmsy kegggpqvat dgpilknfrp pqplngrvvr
      301 epae
//
LOCUS       XP_077503171             304 aa            linear   INV 25-NOV-2025
DEFINITION  carbonic anhydrase 1-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503171
VERSION     XP_077503171.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647045.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..304
                     /product="carbonic anhydrase 1-like isoform X2"
                     /calculated_mol_wt=33919
     Region          48..300
                     /region_name="Carb_anhydrase"
                     /note="Eukaryotic-type carbonic anhydrase; pfam00194"
                     /db_xref="CDD:459707"
     CDS             1..304
                     /gene="LOC144113745"
                     /coded_by="XM_077647045.1:314..1228"
                     /db_xref="GeneID:144113745"
ORIGIN      
        1 mpasrlwtlc snraltipav aikrfvshsa ptqaegfrhl rhrkvvicgp snwakrfpka
       61 agdnqspidi vrsdvqqdpf ltenplrwty tglqctslln tgsgwrvdvs apgpnlrggp
      121 lhnnyqmvqf hshwgtcset gsehtvdgeh yagelhlvhy nvekynkpse aacsdkglsv
      181 vaiffkegkp hhelkkitdc mskvtfkgmk cpleqdidin siipanssyw tyegslttpp
      241 cyesvtwivf kepievsreq feafrelmsy kegggpqvat dgpilknfrp pqplngrvvr
      301 epae
//
LOCUS       XP_077503173             297 aa            linear   INV 25-NOV-2025
DEFINITION  carbonic anhydrase 1-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077503173
VERSION     XP_077503173.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647047.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..297
                     /product="carbonic anhydrase 1-like isoform X3"
                     /calculated_mol_wt=32723
     Region          30..293
                     /region_name="Carb_anhydrase"
                     /note="Eukaryotic-type carbonic anhydrase; pfam00194"
                     /db_xref="CDD:459707"
     CDS             1..297
                     /gene="LOC144113745"
                     /coded_by="XM_077647047.1:116..1009"
                     /db_xref="GeneID:144113745"
ORIGIN      
        1 mahvsaggsg agpslsmclt qllahcwpac gpsnwakrfp kaagdnqspi divrsdvqqd
       61 pfltenplrw tytglqctsl lntgsgwrvd vsapgprdak pinpirlnlr ggplhnnyqm
      121 vqfhshwgtc setgsehtvd gehyagelhl vhynvekynk pseaacsdkg lsvvaiffke
      181 gkphhelkki tdcmskvtfk gmkcpleqdi dinsiipans sywtyegslt tppcyesvtw
      241 ivfkepievs reqfeafrel msykegggpq vatdgpilkn frppqplngr vvrepae
//
LOCUS       XP_077503174             284 aa            linear   INV 25-NOV-2025
DEFINITION  carbonic anhydrase 1-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077503174
VERSION     XP_077503174.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647048.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..284
                     /product="carbonic anhydrase 1-like isoform X4"
                     /calculated_mol_wt=31705
     Region          17..280
                     /region_name="Carb_anhydrase"
                     /note="Eukaryotic-type carbonic anhydrase; pfam00194"
                     /db_xref="CDD:459707"
     CDS             1..284
                     /gene="LOC144113745"
                     /coded_by="XM_077647048.1:349..1203"
                     /db_xref="GeneID:144113745"
ORIGIN      
        1 mskpqeeqpa wgysdadgps nwakrfpkaa gdnqspidiv rsdvqqdpfl tenplrwtyt
       61 glqctsllnt gsgwrvdvsa pgprdakpin pirlnlrggp lhnnyqmvqf hshwgtcset
      121 gsehtvdgeh yagelhlvhy nvekynkpse aacsdkglsv vaiffkegkp hhelkkitdc
      181 mskvtfkgmk cpleqdidin siipanssyw tyegslttpp cyesvtwivf kepievsreq
      241 feafrelmsy kegggpqvat dgpilknfrp pqplngrvvr epae
//
LOCUS       XP_077503175             273 aa            linear   INV 25-NOV-2025
DEFINITION  carbonic anhydrase 1-like isoform X5 [Amblyomma americanum].
ACCESSION   XP_077503175
VERSION     XP_077503175.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647049.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..273
                     /product="carbonic anhydrase 1-like isoform X5"
                     /calculated_mol_wt=30430
     Region          17..269
                     /region_name="Carb_anhydrase"
                     /note="Eukaryotic-type carbonic anhydrase; pfam00194"
                     /db_xref="CDD:459707"
     CDS             1..273
                     /gene="LOC144113745"
                     /coded_by="XM_077647049.1:357..1178"
                     /db_xref="GeneID:144113745"
ORIGIN      
        1 mskpqeeqpa wgysdadgps nwakrfpkaa gdnqspidiv rsdvqqdpfl tenplrwtyt
       61 glqctsllnt gsgwrvdvsa pgpnlrggpl hnnyqmvqfh shwgtcsetg sehtvdgehy
      121 agelhlvhyn vekynkpsea acsdkglsvv aiffkegkph helkkitdcm skvtfkgmkc
      181 pleqdidins iipanssywt yegslttppc yesvtwivfk epievsreqf eafrelmsyk
      241 egggpqvatd gpilknfrpp qplngrvvre pae
//
LOCUS       XP_077503176            1392 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113747 [Amblyomma americanum].
ACCESSION   XP_077503176
VERSION     XP_077503176.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647050.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1392
                     /product="uncharacterized protein LOC144113747"
                     /calculated_mol_wt=151728
     CDS             1..1392
                     /gene="LOC144113747"
                     /coded_by="XM_077647050.1:143..4321"
                     /db_xref="GeneID:144113747"
ORIGIN      
        1 mpkvkshlvf ltllclwsgc fatarrrkgr dvpdsckneg vrvsecrasf lteidmmdka
       61 knfqhtnctl yskleccmvt eygslgcadi dvinkaiart ikdfpsyenc prglcdpasv
      121 ngsfkimrdm denlhgrdav sglvsslkrk vkrevsagme sarsevssev sksyveehlk
      181 gpkhrgkkkg hkrghkgkks kkpklaaddl prsaripdhe kgdasvhdvt ekthedqltk
      241 aaspaalnea shdkaqqnis sgasalegns natdvtkkse vvsasagfsv ppapaaaadg
      301 sstnasglen aavtgktagl dglsdikvpi sievvknisl sdigktpkks hrrrrhhknk
      361 kaalnvdatk rsevegvtke hesevtasvp sldstfggea apapgaalsa danqssragp
      421 nnelpqpgtp easdhgrekl dakesiisns ssisstnksi vdaspqptlg evgvteakpd
      481 sakellades rgsppassds vgnkpnvdqp ltdknqsdda sarthlatnk sekltkddvn
      541 ktlsdkdtnk tagaiaespk pdakldtaap vvdattmvqe anlddkhtet lspmsekkev
      601 qdndlsnlpk esksklvsev ndpntklegn tstgsgltkn ssvnsnessp lspdarhste
      661 kntnhsaeek eakvnatsek aepknsklsd vpeislavhr lekkhelssg vnsfghksle
      721 rgstgkektn qtkgsevkne teshegivna dashnqtlna gashnqtqke pgaeglpasa
      781 kghsdnktea lsetavnlkd pkqvfgsgpl ievkgnkggs vekqmvhaye tgdsvesfkg
      841 knsliddlvd qygpgssdyd frygrdmkrk hkprfrdddd apkvrrrqwd gdepfevdtt
      901 kdpfqeypgm kdgdprtggl yvaerkhrrp adesvkiarh kkvhrrkpen dynyesvvrw
      961 klleeldsil dpknspevea lnkpsepefe hekdddmdqw krrlepfkvq veeqsgehll
     1021 gsgddsfrda evmrdgemyy rkyrrqgpvv ygqgyegver pivkdplihq pkkrfwierq
     1081 esdsmkrpah gedtqsavdi hlrplpgvpg hdaggadaae rsigfaqesa peesphrais
     1141 aegrasqlfr ggvgrrlinr raararrlrg srasrklrkn lkdteglkil rnddlqgddd
     1201 ddddddsddd ddddddddek evvaikkstl apitakgqvg rvdaaerplm sfgerihtve
     1261 ptrptpkkdf kpsgslvgsa espdcvpdtl rahtdycrgi ytkhqlsiee arktaskktm
     1321 acyklesiks clsagsyfsk cvgsnnnefq dlqgsvmtqm rdlrcgasvi ssssllllla
     1381 vaanyflrre nn
//
LOCUS       XP_077503177             893 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113748 [Amblyomma americanum].
ACCESSION   XP_077503177
VERSION     XP_077503177.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647051.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..893
                     /product="uncharacterized protein LOC144113748"
                     /calculated_mol_wt=97992
     CDS             1..893
                     /gene="LOC144113748"
                     /coded_by="XM_077647051.1:184..2865"
                     /db_xref="GeneID:144113748"
ORIGIN      
        1 mdlklvalca ilfisrgacg arvenvttkg lpsgwfpgaa vendtkcnyl qlaktytrcs
       61 lpfyadfvsd tlhsepstcs pvktfetcle dalkntlcsn dttlltptvk fftssilgay
      121 kqacnaalgs knftpsvakn asvggekspi vhdtsstash dnllisnnsl vteinlknas
      181 aakelnegdn htetfpavea nrkafvddkp sadntsavft kgvtfrdlvg vtnaadkglg
      241 shndsanver rmrrsdndpc epkidtgfkf pekcpnrtll rrmftcgvam hefilhgqnp
      301 ckcflnyrdc vekarkeldc esggakddpi ivtvkliesl mlswyqnpca iyrdpanrts
      361 vrarivdeve ggcnvrkasi atvvcyatyf amtelktpap wtnhddvcsa fidvnrclva
      421 aqhksnctni eftdhsrkfl aiitskqhrg cpgeagmpnv qavaayvkpg cqrskalkra
      481 lrcsisfqdl adterefapp qpvpcghvsr lhtcledsve gtgcygdinv hleikvykkv
      541 ledsydvrci vegptekrrr aknrqrilrp hpktlykqms gssglrswrv dlglyeknde
      601 nagyyyddsl egrkpvfagp rviaihpdda eeegdyensd alyqkahneg gggwrplaqr
      661 lkeeqdlaak vvevngtsne ttpqallqff kpensealaa qeqpksrsaq lqkleelakk
      721 phgkkaaged difgneyven selamfpngl dmfhhfrgaa larmenvvea nksesasddc
      781 pveqvklalg lcnatlkgll lrwpnvsdvg lpldgssagv kqfcsdlnty kgclasvlka
      841 yscsegqafn giyeayrkqm rlpycaassa twnnaavctv figllasvvn kyi
//
LOCUS       XP_077503178            1671 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113749 [Amblyomma americanum].
ACCESSION   XP_077503178
VERSION     XP_077503178.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647052.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1671
                     /product="uncharacterized protein LOC144113749"
                     /calculated_mol_wt=189355
     CDS             1..1671
                     /gene="LOC144113749"
                     /coded_by="XM_077647052.1:426..5441"
                     /db_xref="GeneID:144113749"
ORIGIN      
        1 marlhclivf iallwaptdg lafakakaga kakcqrfhal krfsacgavv ghavreksll
       61 ehpnqnatcq kaqgfekcvq dsmrltrcvr ssefnfhlqh isslvnemyk escsssgets
      121 smltdmstvc dirvavvrfl ecsssfyigq pqpapvlnqs qevsattlgv hfgvifdapa
      181 vsgtldkynh lpsiecrrsa kfrncidnih lysecgigte vhshlkylan ivnrkfvgkc
      241 sqqtqipdld cqllpflrkf lrcgvnryet agdrvdrdic tqvisykecv aaaervthcn
      301 mrnlslsyhl nylhkvmtsv ntscngarnv etsvykelck rttlfnnyfs cgmevqarle
      361 glkkgdkerc risenfekcf inakeqsgcl ladeatsims alldtwkkeh akecadfdvp
      421 pprggaqadc ltgtavrkvf lcglsfqtav revkfsplam spevcrrlde mntciaevkg
      481 rtqcamselh ahvapllqpf tidyaercek mvwsnqsapm laptaqtcrr lpalkrifhc
      541 glsfsrilee melvhqkaev lcpllkkyet cvpdsaselg ckndpvmkgh vrsfgkilhr
      601 qyyeaclrnr rgimptrlea lrrgrmkken strdifgtvn ilkqelkrgr nvsaniqeqi
      661 lsqvmedyyg pdydeyveee dkvpdsldvk paydytdreg tpkaknqdap itaqsprgaa
      721 pnssarpaek qdgnydprrn qefwagvera rrlrsqhkhe sgsaelihdi snkranldfa
      781 rpgayfidql gnaqdpredm yakkeqqteg tienpgrrpl pehywnppas favyrplrnv
      841 phaarqgerd vegwrggppl sreeelemye dtrgmsdllr kqrgahygry ngrgglgprs
      901 dlqraekesa hryalgdaaf pgsynyakss lngsvpyyey hgssgvqpfk teyvgrrieh
      961 rpyitlygpd drpgegqqdd srlipvrpdr rvismqrrgl dyrsadydpr eyvksplapl
     1021 phlagegvas rdmasfpddd gpalfneepl spngkqmglr ravpirrrdg kppldtarnl
     1081 egtagmiysp srrypenvpg aangprldam dnvkeefpqd rrqggalpyy prrlaskkfr
     1141 alagidprsl vgimqedydr gsrdhnrasl rmndepflpg neeisprlna mdrlsprdqv
     1201 gppknpnyyv peespgnarp fqrkfsfkrp ayketkpsnr esypdetrpl pntpqrhtnd
     1261 sshvgpnsfl pepglkspke kvvpdflgdp gvhttrpetr lrkedpprrr alnspdgrmk
     1321 aldsgtdfea dqnshseefl qrgakgqkrf fevkkrrpep rlgfpyvrhi despglllek
     1381 kdeasggaiv kqkpqnnnhq ltqyelerkr kkklikddld eivskhreds dskadmmlfp
     1441 serlphddrg vrdfdarale enkdyyeadt klegdtgdrs amwsaggdda rdelvrrhmq
     1501 hvqrkdrkqd klmgelsrrs awytddiwsd sndlfgmerl lsdqrhdggd niteedetsq
     1561 cllhnlqrrs dacvrvfedd fralgngter nfsaqaltpq lrksvcshar dfsqcmnvfa
     1621 qkfrcvdsqe lisnlteehl tragvmfchs stacisvrlm laalilhfls l
//
LOCUS       XP_077503179            1671 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113749 [Amblyomma americanum].
ACCESSION   XP_077503179
VERSION     XP_077503179.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647053.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1671
                     /product="uncharacterized protein LOC144113749"
                     /calculated_mol_wt=189355
     CDS             1..1671
                     /gene="LOC144113749"
                     /coded_by="XM_077647053.1:430..5445"
                     /db_xref="GeneID:144113749"
ORIGIN      
        1 marlhclivf iallwaptdg lafakakaga kakcqrfhal krfsacgavv ghavreksll
       61 ehpnqnatcq kaqgfekcvq dsmrltrcvr ssefnfhlqh isslvnemyk escsssgets
      121 smltdmstvc dirvavvrfl ecsssfyigq pqpapvlnqs qevsattlgv hfgvifdapa
      181 vsgtldkynh lpsiecrrsa kfrncidnih lysecgigte vhshlkylan ivnrkfvgkc
      241 sqqtqipdld cqllpflrkf lrcgvnryet agdrvdrdic tqvisykecv aaaervthcn
      301 mrnlslsyhl nylhkvmtsv ntscngarnv etsvykelck rttlfnnyfs cgmevqarle
      361 glkkgdkerc risenfekcf inakeqsgcl ladeatsims alldtwkkeh akecadfdvp
      421 pprggaqadc ltgtavrkvf lcglsfqtav revkfsplam spevcrrlde mntciaevkg
      481 rtqcamselh ahvapllqpf tidyaercek mvwsnqsapm laptaqtcrr lpalkrifhc
      541 glsfsrilee melvhqkaev lcpllkkyet cvpdsaselg ckndpvmkgh vrsfgkilhr
      601 qyyeaclrnr rgimptrlea lrrgrmkken strdifgtvn ilkqelkrgr nvsaniqeqi
      661 lsqvmedyyg pdydeyveee dkvpdsldvk paydytdreg tpkaknqdap itaqsprgaa
      721 pnssarpaek qdgnydprrn qefwagvera rrlrsqhkhe sgsaelihdi snkranldfa
      781 rpgayfidql gnaqdpredm yakkeqqteg tienpgrrpl pehywnppas favyrplrnv
      841 phaarqgerd vegwrggppl sreeelemye dtrgmsdllr kqrgahygry ngrgglgprs
      901 dlqraekesa hryalgdaaf pgsynyakss lngsvpyyey hgssgvqpfk teyvgrrieh
      961 rpyitlygpd drpgegqqdd srlipvrpdr rvismqrrgl dyrsadydpr eyvksplapl
     1021 phlagegvas rdmasfpddd gpalfneepl spngkqmglr ravpirrrdg kppldtarnl
     1081 egtagmiysp srrypenvpg aangprldam dnvkeefpqd rrqggalpyy prrlaskkfr
     1141 alagidprsl vgimqedydr gsrdhnrasl rmndepflpg neeisprlna mdrlsprdqv
     1201 gppknpnyyv peespgnarp fqrkfsfkrp ayketkpsnr esypdetrpl pntpqrhtnd
     1261 sshvgpnsfl pepglkspke kvvpdflgdp gvhttrpetr lrkedpprrr alnspdgrmk
     1321 aldsgtdfea dqnshseefl qrgakgqkrf fevkkrrpep rlgfpyvrhi despglllek
     1381 kdeasggaiv kqkpqnnnhq ltqyelerkr kkklikddld eivskhreds dskadmmlfp
     1441 serlphddrg vrdfdarale enkdyyeadt klegdtgdrs amwsaggdda rdelvrrhmq
     1501 hvqrkdrkqd klmgelsrrs awytddiwsd sndlfgmerl lsdqrhdggd niteedetsq
     1561 cllhnlqrrs dacvrvfedd fralgngter nfsaqaltpq lrksvcshar dfsqcmnvfa
     1621 qkfrcvdsqe lisnlteehl tragvmfchs stacisvrlm laalilhfls l
//
LOCUS       XP_077503180            1671 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113749 [Amblyomma americanum].
ACCESSION   XP_077503180
VERSION     XP_077503180.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647054.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1671
                     /product="uncharacterized protein LOC144113749"
                     /calculated_mol_wt=189355
     CDS             1..1671
                     /gene="LOC144113749"
                     /coded_by="XM_077647054.1:196..5211"
                     /db_xref="GeneID:144113749"
ORIGIN      
        1 marlhclivf iallwaptdg lafakakaga kakcqrfhal krfsacgavv ghavreksll
       61 ehpnqnatcq kaqgfekcvq dsmrltrcvr ssefnfhlqh isslvnemyk escsssgets
      121 smltdmstvc dirvavvrfl ecsssfyigq pqpapvlnqs qevsattlgv hfgvifdapa
      181 vsgtldkynh lpsiecrrsa kfrncidnih lysecgigte vhshlkylan ivnrkfvgkc
      241 sqqtqipdld cqllpflrkf lrcgvnryet agdrvdrdic tqvisykecv aaaervthcn
      301 mrnlslsyhl nylhkvmtsv ntscngarnv etsvykelck rttlfnnyfs cgmevqarle
      361 glkkgdkerc risenfekcf inakeqsgcl ladeatsims alldtwkkeh akecadfdvp
      421 pprggaqadc ltgtavrkvf lcglsfqtav revkfsplam spevcrrlde mntciaevkg
      481 rtqcamselh ahvapllqpf tidyaercek mvwsnqsapm laptaqtcrr lpalkrifhc
      541 glsfsrilee melvhqkaev lcpllkkyet cvpdsaselg ckndpvmkgh vrsfgkilhr
      601 qyyeaclrnr rgimptrlea lrrgrmkken strdifgtvn ilkqelkrgr nvsaniqeqi
      661 lsqvmedyyg pdydeyveee dkvpdsldvk paydytdreg tpkaknqdap itaqsprgaa
      721 pnssarpaek qdgnydprrn qefwagvera rrlrsqhkhe sgsaelihdi snkranldfa
      781 rpgayfidql gnaqdpredm yakkeqqteg tienpgrrpl pehywnppas favyrplrnv
      841 phaarqgerd vegwrggppl sreeelemye dtrgmsdllr kqrgahygry ngrgglgprs
      901 dlqraekesa hryalgdaaf pgsynyakss lngsvpyyey hgssgvqpfk teyvgrrieh
      961 rpyitlygpd drpgegqqdd srlipvrpdr rvismqrrgl dyrsadydpr eyvksplapl
     1021 phlagegvas rdmasfpddd gpalfneepl spngkqmglr ravpirrrdg kppldtarnl
     1081 egtagmiysp srrypenvpg aangprldam dnvkeefpqd rrqggalpyy prrlaskkfr
     1141 alagidprsl vgimqedydr gsrdhnrasl rmndepflpg neeisprlna mdrlsprdqv
     1201 gppknpnyyv peespgnarp fqrkfsfkrp ayketkpsnr esypdetrpl pntpqrhtnd
     1261 sshvgpnsfl pepglkspke kvvpdflgdp gvhttrpetr lrkedpprrr alnspdgrmk
     1321 aldsgtdfea dqnshseefl qrgakgqkrf fevkkrrpep rlgfpyvrhi despglllek
     1381 kdeasggaiv kqkpqnnnhq ltqyelerkr kkklikddld eivskhreds dskadmmlfp
     1441 serlphddrg vrdfdarale enkdyyeadt klegdtgdrs amwsaggdda rdelvrrhmq
     1501 hvqrkdrkqd klmgelsrrs awytddiwsd sndlfgmerl lsdqrhdggd niteedetsq
     1561 cllhnlqrrs dacvrvfedd fralgngter nfsaqaltpq lrksvcshar dfsqcmnvfa
     1621 qkfrcvdsqe lisnlteehl tragvmfchs stacisvrlm laalilhfls l
//
LOCUS       XP_077503181            1095 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113751 [Amblyomma americanum].
ACCESSION   XP_077503181
VERSION     XP_077503181.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647055.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1095
                     /product="uncharacterized protein LOC144113751"
                     /calculated_mol_wt=123525
     CDS             1..1095
                     /gene="LOC144113751"
                     /coded_by="XM_077647055.1:183..3470"
                     /db_xref="GeneID:144113751"
ORIGIN      
        1 mantavasll tavvlalllg rveasknvwi lskqemegdg cdfrayektr nacedmfsnv
       61 dvvtsgnntc snvqiyitcv kdamrktlcs andtikenen ariminvrkk nvtcipkaap
      121 qnmrqtmgcq rsvalkkffa csstfhyymk nkddllpsgg gtdcsvvsgy kdcvqlglrh
      181 sdcaddkdfi dhvhyfmrnd ivvfakpcqv dnnspaanit alktcdedka idtyltcakk
      241 ffdnvdseel rklskfpqka ksnsqlvgsg lrsneltakp efkvdpctav kswlncyydd
      301 llksncstds elykrsqaly qvmvdefncv ntqfqavaes etpdcqekpm lnnffvcain
      361 ynverkripv slqtkpdvic psnlkfemcv aqamresrcl hermkimhhl kyltdvittp
      421 tvfckegaae yakqvldgnk rcsrttlfre yftcglsfqk elethnnfqt eesgkercat
      481 mnaynecfrk aveasechsk saitdtmqyl meletkdidy sceeprpvln agiistrcak
      541 slavknaflc amtfehlved lkvkreatae kvckyleemn scvdvvkkst gcdrdgeifn
      601 hvspilhslt heydracrnv tnsfrmsfvq mktpencvrq rvlkkmfvcg lsfsnlfdem
      661 eprsesgnpv kckllidyeq ciakasrgtg cdedaemrvq imsfsdimrd eykdscpnvv
      721 yknapeaaeg fedyeedwae sdpywydtke gadgqtpgka rkpsagvvkp redpfgnvkd
      781 halsnasyvd aagkpalkrv drerilqesn kpatlrqqag pgqnlsdvmd lstflkrdsk
      841 svfnasgkrk vriekeyldd spaamasygl gadykeeyad dtfarvpsrr mglryrplqr
      901 rgdpnawvss rramkildde eqqedddimg lmrrkrkrtg sdavrrlgkd lqvkkqldyd
      961 degeedeilt drethisgde cnlkqlkrqs etcdrtfsds ikvswskdwp apassssstp
     1021 akdvqeklcn slvtyrtcll etakrmhcge iagkvavvve eqmkklgvaf cgaaslrmaf
     1081 wlaallsmal iflqa
//
LOCUS       XP_077503182             996 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113752 [Amblyomma americanum].
ACCESSION   XP_077503182
VERSION     XP_077503182.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647056.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..996
                     /product="uncharacterized protein LOC144113752"
                     /calculated_mol_wt=111757
     CDS             1..996
                     /gene="LOC144113752"
                     /coded_by="XM_077647056.1:308..3298"
                     /db_xref="GeneID:144113752"
ORIGIN      
        1 mrkgaptttm pvsglmayal ilmtctsaed talnapslrg qegeqcqfte fsrrredcnk
       61 gvsflsarkg ddchimqvya dcvtlalrtt rctknpffis sesnvirtkm adkklscdlh
      121 sdllilqvda ahsclrvrav klffkcgtaf hfhmggkslt patvcdllkt yvdcghaalk
      181 dtnctkeenl kahmdfflaq slkrhsircp nittynvssp vlkgavvavp esqrwamays
      241 qdgstdvrpr kheplmkqtk eclvedalrr yftcgsnflw kmviwkrhfa khqhmynfpt
      301 ndtlpstpsl kmlarrasen devcgsikel hectneilsr sdcknpdgla akmvtmqeml
      361 vgdasksack fspktkhgvy psyskqnlpp pgcsyrklvk lfsfcvvnff ssfamskdnm
      421 qaclfvgrhg scveqarkst ncsmqvkeld dfvyltnvvq dkyssrctpd gqktlaekka
      481 eagvpslckr ksllqrylkc shtlqdklsv kvddcralns fsqcfmeara dnectftedg
      541 ftsimqmqae lmtdtlrsrc tqpfdlnvll kcaqhdalkk vllcglsynk lmedlkrqge
      601 yyvtsttcsy laeyqhcive verstgctks tlhehtsail nfwtwnvapl cesaaslllt
      661 dvdpkcdspk allkalkcti efqeyaeqaa wdtmtsaesc sslemldkcl sssleigqck
      721 nslnakaqvs alrsllvsey rvgcrkiyls apgmpgmrvd dyvyedyyyf gdevlrtkpp
      781 kkranvfgka llrkepqiap sarsklrdei rkifnkirgm gfdkilskvk pdfghavvee
      841 mtaavpistt nsseeyplhf litgavipvm daddatcdia gyqeererct qyiadqadky
      901 rmnikyipvs nlrditdtvc rmvdlyracm sevakrnkcl amlantpriv enqlmklglt
      961 fcssatidsg cssrhsavqv tvailsvfcl kmknwe
//
LOCUS       XP_077503183             852 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113753 [Amblyomma americanum].
ACCESSION   XP_077503183
VERSION     XP_077503183.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647057.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..852
                     /product="uncharacterized protein LOC144113753"
                     /calculated_mol_wt=97268
     CDS             1..852
                     /gene="LOC144113753"
                     /coded_by="XM_077647057.1:220..2778"
                     /db_xref="GeneID:144113753"
ORIGIN      
        1 mkafvrllgv tlyfvacdgf qvvlqsaqhq gpkydsgcdv arftpqrelc datfklniph
       61 nhgdmkfvcs vigkykmcla nviqatgcdn keflveqlqp mqqyiqqnri ecipdvnsss
      121 varipagyra kldlctrdka wetqflcakk fhtklrkied dkeveshqic rslsryyscl
      181 ntvlhsesce edtelmmhme yfpkvltqky remcfaelkl tamnvakrle gytmdqtcqe
      241 edatkqffac gllfneiish sppqericla yrdfenctnv iardlhcnvg sefsrhsmhv
      301 mnvlvsqyeg ycrtirptnp idetgqpgpt pitpgpgpgp svtvqppvgc dgdlymekyf
      361 ecgltyifsl rdamfgdnph hqdqicetit lhksclegnk ktsrcsdlpe ikasldhinn
      421 elwnapgakc nsaqykrsgr irfrtseqqc vvreyaatff tcgtiflknt ypqavskded
      481 crfyreflrc keylipcksq sdissaldhf stvltegyes kctgynitdt cnklvliknf
      541 fscgltyyqs ynefgatylv aephacklly dfhncsyesv lknkpncket rdlfdnikkv
      601 kdyingmfnt khksdcktpa gmekrrvylg lerqsacdqf kaikklvlcg vtfhrmllti
      661 engtnakenr srvcplvkem kycmyaathd sgcsdalfln teisvlkkhl lmefedscnv
      721 lpsgddqefk vyrqacelke ftqewescda ameddigify rngsvfrkns msnrvrrrlc
      781 rdligyrkcl nnsadkhhcs plapqvsems nelfdrlglv ycsggtrhag lwslivaiaa
      841 swlmrqsadr ps
//
LOCUS       XP_077503185             399 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113754 [Amblyomma americanum].
ACCESSION   XP_077503185
VERSION     XP_077503185.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647059.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..399
                     /product="uncharacterized protein LOC144113754"
                     /calculated_mol_wt=45625
     CDS             1..399
                     /gene="LOC144113754"
                     /coded_by="XM_077647059.1:117..1316"
                     /db_xref="GeneID:144113754"
ORIGIN      
        1 mtkavlwpfc flflllmklc sgvpcngesy qklkrqctqa lhqrvkevqg dltrncrdfa
       61 dyvvcvrdsw risycdneta larhndtlve gmqkkyfrnc yndtsaqrke crpdkafekl
      121 ltcgnrfyds avhvsnkrsa islpslcpev ekyglcayks qqdancslgy qmvphlkyia
      181 ehieqryrll ctalrrsafq rgttacdrqr findmflcgy hfanvaevvg gnpqevcpyi
      241 ekykechnts iqtygcappd hlyqdaekff vlltqkynst cgsqgiarnf pqasrltlkd
      301 gagytcdgnr firyffqcaa gfvnatgnsl kdmhyicrav giyrhcvnga rhathcntkm
      361 elnshiafmq nfileqyepm camkrgktsg esdskraer
//
LOCUS       XP_077503186             271 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113755 [Amblyomma americanum].
ACCESSION   XP_077503186
VERSION     XP_077503186.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647060.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..271
                     /product="uncharacterized protein LOC144113755"
                     /calculated_mol_wt=28467
     CDS             1..271
                     /gene="LOC144113755"
                     /coded_by="XM_077647060.1:484..1299"
                     /db_xref="GeneID:144113755"
ORIGIN      
        1 mmysqvplfl vyaiqpydls qgeykteglt kntvtsnegt vngltgseal avvnevgkkk
       61 isapfltwys skkgsqeeqs qskrrvtrng gwratqqsav qslqpfgsls agqdgvqaqv
      121 nsaqltklaa glsqaaslse aavqqtseeq qssaardssv ssrgyyrgss hefsqlgasd
      181 tsaeqqqehs sqeeaavqtg eaaeaqqalp eapsasvqsn lfadqgsgqq tassswstgi
      241 rhqktaqagg tkggtkgllr awtrnnlgys g
//
LOCUS       XP_077503187            2145 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC144113756
            [Amblyomma americanum].
ACCESSION   XP_077503187
VERSION     XP_077503187.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647061.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            internal stop codons :: corrected 1 genomic stop codon
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2145
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC144113756"
                     /calculated_mol_wt=238315
     CDS             1..2145
                     /gene="LOC144113756"
                     /coded_by="XM_077647061.1:244..6681"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: substituted 1 base at 1
                     genomic stop codon"
                     /db_xref="GeneID:144113756"
ORIGIN      
        1 mneprialhf llwlsisvps caveylpraa dnpaehrlsa cdqnvliqft necekvfndv
       61 igsspdnttv kcsyfrtyhs cvhdalrstr cdgeslivdk alatieqhrl vnrlncdrdh
      121 ivyapqindq svacrkrsal rkfflcglay hyvtekndsr tggdwrlmcq avheyqkcts
      181 malsstdcfm dealrrhvsy fssgaldeqg vacssdgmpk aqrsqisskq qrqmnmaqrq
      241 cvgraalkdf fncglefnrg vklvslntgp kvrraysrkk grrssrrrqk pivvprgafr
      301 kpllragnqe mclivekfkn ccnaleerhk csagsqdlyg hmtfvqktli eslgelgcgg
      361 lpppdhttti gtrgppgppi tfgtdtivpp mftmptgips gcayvqlvrr ysycavafya
      421 dlegrgvtat sqdvcnfvne flecqdkaee etkcnrpknq fqdlnyftdv llkkyqsqch
      481 lttrftkpdr ptgpdgrptt pmgpvpptgc nqkdllrrff acgialqasi evadepckci
      541 teyekcyara kdqlqckddn mgknftyyml elteatkqmy nvaqcpksfy aggitagkcd
      601 ateamrrffl cdlsffrvve naasaggkaa qdicryvsdv neclakleyq tgctrsalhk
      661 hatsllnkit spfsrrcsrg kgkxtwktna qvalqkekcd esgvlkkalv caigfqnfmd
      721 qkvsrrrptk eevcpfikdf encidnasqg stcrtdmsls aqlvtykrll eeeygvdcrv
      781 rdsgalsrrf sagsdsgdys yddsrkreqk sqrnshherp qpasgrkads yeyddgprrr
      841 ggvrkaapsr pgaykgredy dyyndqakam ptpplpppnv eyededakey ydfkpdvkrg
      901 dqsgdggrgt rgrlrrgped derpsrkskr adgrskggrk neapqekaak dhayvdddde
      961 spvrpgssyd ygfeegfskk klkrndrned asffeddqds eqedrrrnrr qdapalgnvp
     1021 ddddetldee rmnagldaek nirgsrrarv ddedddedpf frgklarakq drkfqrsrym
     1081 qmeddgyeea qrrknlsacn vhmyhidmkr cdrgimdeva slynvtvrls rsvsnaakss
     1141 aciairefes cimqaqikhh cmvfdethni svmrnllara fdvncsdtap vpmkqvyrss
     1201 rqskcnstll arqysycavg fyaevegqqi gvtdkavchf vqefmnctkd aykdancegv
     1261 nveikpttgf fdnvllakya rmcgiekdvt rpttpqggts ptgggtnpid gdcpapkpgs
     1321 pcgpygpggp kgpcgpggps ssciaepgqp ggpggpggpg gpggpggpgg prpgepgspg
     1381 tpggpggpgg pggpggpggp ggpkpgepgg fggpggpggp ggpggpggpg gpecicpttl
     1441 splfttpttt tsttppptag tkpppgptpe eetetdtpwt kgptpstttt tektftsttp
     1501 svtgvtmett cinintsfpy psdcnkkell rrfftcgitf qtnlelqedt ckcyynykkc
     1561 fdearkelkc hetdqtkdpl vitvnilsel miniysvkce psmsplnfee cnqamalkki
     1621 mmccvtyfsl sdehakssws gsadvcsyaq evhrciaiae hktkcvnhrl fdharefvrh
     1681 atsdkrhlcs lnissmsiqa lgphmkskcq htavmkralt calqfqntld ghikskppma
     1741 lacgyvtrlq eclssslegt ecvgessvna qlkvykrvlq dayslncdts rpkevkagrq
     1801 qhtqkkadar msnkksafrm gkdlrqnalr ryldknarms kqtggrgsyd edddyelisk
     1861 rqkpvfmspa gklmeepdgf rdddsyelvr sdgyqyhdln rknqreledy edfadlepdv
     1921 rpragrkshr rhrklrnrdg kakklvvdsl vaskarhskr dkrrklayed eyffdnepee
     1981 esvisglpen mamfqhikhr pemnverlls gnvttnasit tdmagqkeyv cnsadlqnev
     2041 nkcnvtltkl itlwpqsykk qrdlimskqa ydaticsdvr synacltfam qatgcgdhlp
     2101 tlrqiieeqq srfglgfcts gnkrshpstf fflwvvlfal lhrgq
//
LOCUS       XP_077503188             331 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113757 [Amblyomma americanum].
ACCESSION   XP_077503188
VERSION     XP_077503188.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647062.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 39% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..331
                     /product="uncharacterized protein LOC144113757"
                     /calculated_mol_wt=36640
     Region          <8..80
                     /region_name="transpos_IS481"
                     /note="IS481 family transposase; NF033577"
                     /db_xref="CDD:468094"
     Region          <28..214
                     /region_name="COG4584"
                     /note="Transposase [Mobilome: prophages, transposons]"
                     /db_xref="CDD:443641"
     CDS             1..331
                     /gene="LOC144113757"
                     /coded_by="XM_077647062.1:1..996"
                     /db_xref="GeneID:144113757"
ORIGIN      
        1 makelcplts lssteviyhf kkvfsthgip etlvtdngaq fvsqefsqfa ncygfkrvtc
       61 sprypqange vermvktvke lllksgdlhl allayrdtpg plgktpakll mgrrlrttlp
      121 lhtkklapkt pnlkearekd aafretqrqn ydrrhaarhm fplvpgdsvw vkdyrrkgvv
      181 lqrakrprsy ivvlesggqi ernrralikq apnrgsdskd geyeflssld gteedgnsvs
      241 ieqtvpdgpe engtealpsd plqpppetss paaqgehvtr fgrklqaprk qttacamhdg
      301 lrfamngedt qivplanend sshgpadshg t
//
LOCUS       XP_077503189            1004 aa            linear   INV 25-NOV-2025
DEFINITION  protein tiptop-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503189
VERSION     XP_077503189.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647063.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1004
                     /product="protein tiptop-like isoform X1"
                     /calculated_mol_wt=106816
     Region          434..>510
                     /region_name="zf-C2H2_2"
                     /note="C2H2 type zinc-finger (2 copies); pfam12756"
                     /db_xref="CDD:463690"
     Region          434..456
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     CDS             1..1004
                     /gene="LOC144113758"
                     /coded_by="XM_077647063.1:441..3455"
                     /db_xref="GeneID:144113758"
ORIGIN      
        1 mvirsasaea vaviapavta atasvtttrr rprkapprps crlslvdavv aglaaaaakk
       61 hqweegeaka dapadeaked gsvtsdtrst sppaetppae prsspvdser asaatpkapf
      121 spmqdeepig epsapkeepe epprktstpa rdeappilap mvvvkkeeed eedeedapld
      181 fslkkpsgsd vaesgdedma aaegpaanga ttdspldlsv srrrartvph ehkpaklgrl
      241 epvpggpwlg armpsasapv glgaraigse phhqhqswng kvkrerstss elshptgsam
      301 kmafsektkg dafrpasnrq npwqsqwisr sgeqtkdvft cvwckesfqs ladmtlhmkq
      361 sprcgmagmh aaappppsaa aghttppppk ltatppgasg attkdgvplp rklvrgqdvw
      421 lgkgaeqtrq ilkcmwcgqs fksladmtth mrvtqhytni isqeqiiswr tpedklanqs
      481 qvnavltckv cdqafgslke lsyhmvknsh ykehilrsit egggrrrqtr errkkslpvr
      541 kllelermem tarsgapsnq tspsplgaae sdgkiaceec aekvdakdfv qhikscsrsq
      601 qllktalqqq trpepcssne srksaeddtp pppppplesp rpqsaepdra astesekrpn
      661 stsssstsvl naierlieks fdskgkrggp tsagilqrlg ideevyppwh pagsprygai
      721 ksaghsprlt gglaspcpse etpanhaspr sgvsspdkda eppvldperl sqprspaesv
      781 strasspgad dlviadglps rrsserggpd tddlsssssc dtplaemksa spkakqspra
      841 aassrprrgq hngegdavsd hplkelqkll dktdahlsra napqaaspgs ilafswacnd
      901 aatsdslmkc afcdthfvsk gayrhhlskm hfikdgsaap ppppqpgdsp twkakepppa
      961 agshgpgspa aspasspaap adesphskfl kytelakqls skyv
//
LOCUS       XP_077503190             955 aa            linear   INV 25-NOV-2025
DEFINITION  protein tiptop-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503190
VERSION     XP_077503190.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647064.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..955
                     /product="protein tiptop-like isoform X2"
                     /calculated_mol_wt=102274
     Region          385..>461
                     /region_name="zf-C2H2_2"
                     /note="C2H2 type zinc-finger (2 copies); pfam12756"
                     /db_xref="CDD:463690"
     Region          385..407
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     CDS             1..955
                     /gene="LOC144113758"
                     /coded_by="XM_077647064.1:113..2980"
                     /db_xref="GeneID:144113758"
ORIGIN      
        1 mprrkqhrpq rmqweegeak adapadeake dgsvtsdtrs tsppaetppa eprsspvdse
       61 rasaatpkap fspmqdeepi gepsapkeep eepprktstp ardeappila pmvvvkkeee
      121 deedeedapl dfslkkpsgs dvaesgdedm aaaegpaang attdspldls vsrrrartvp
      181 hehkpaklgr lepvpggpwl garmpsasap vglgaraigs ephhqhqswn gkvkrersts
      241 selshptgsa mkmafsektk gdafrpasnr qnpwqsqwis rsgeqtkdvf tcvwckesfq
      301 sladmtlhmk qsprcgmagm haaappppsa aaghttpppp kltatppgas gattkdgvpl
      361 prklvrgqdv wlgkgaeqtr qilkcmwcgq sfksladmtt hmrvtqhytn iisqeqiisw
      421 rtpedklanq sqvnavltck vcdqafgslk elsyhmvkns hykehilrsi tegggrrrqt
      481 rerrkkslpv rkllelerme mtarsgapsn qtspsplgaa esdgkiacee caekvdakdf
      541 vqhikscsrs qqllktalqq qtrpepcssn esrksaeddt ppppppples prpqsaepdr
      601 aastesekrp nstsssstsv lnaierliek sfdskgkrgg ptsagilqrl gideevyppw
      661 hpagspryga iksaghsprl tgglaspcps eetpanhasp rsgvsspdkd aeppvldper
      721 lsqprspaes vstrasspga ddlviadglp srrsserggp dtddlsssss cdtplaemks
      781 aspkakqspr aaassrprrg qhngegdavs dhplkelqkl ldktdahlsr anapqaaspg
      841 silafswacn daatsdslmk cafcdthfvs kgayrhhlsk mhfikdgsaa pppppqpgds
      901 ptwkakeppp aagshgpgsp aaspasspaa padesphskf lkytelakql sskyv
//
LOCUS       XP_077503191             810 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113759 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503191
VERSION     XP_077503191.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647065.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..810
                     /product="uncharacterized protein LOC144113759 isoform X1"
                     /calculated_mol_wt=88208
     Region          <109..232
                     /region_name="PRK10856"
                     /note="cytoskeleton protein RodZ"
                     /db_xref="CDD:236776"
     Region          582..804
                     /region_name="TACC_C"
                     /note="Transforming acidic coiled-coil-containing protein
                     (TACC), C-terminal; pfam05010"
                     /db_xref="CDD:461517"
     CDS             1..810
                     /gene="LOC144113759"
                     /coded_by="XM_077647065.1:160..2592"
                     /db_xref="GeneID:144113759"
ORIGIN      
        1 middpssekk enlppdgkps tsrrksteke qrlsvgassd pqesliaara ilsdivnhvv
       61 sersprptea sadngnsvln rtlssrslks yllagqcvpd edcanrtfpl eadpegserp
      121 sptsanagss gpadtgvvte psgsaradna lrpctseasa easpcvllsp enrssdahpv
      181 asrsatpvdt dqggsasdsv seppssdcwy ytdasdaslp gvgankseld ltyttakgsp
      241 lgaarffdts slhtsnvaps edtevnhpag safnescdqe eafcdsqpad alelhdghle
      301 tsgnvtcdvl rlqdsesspe stlkllspgl iektfespsk nfgvlapsdl qkastpttqe
      361 dvvphfdyhc svqsptdfcn ktckaseass hletpththr qdetvtlsat gfnenllsss
      421 dpsnaevtvi ekdpatpaav lgneatahiq chpdkgtteg tlpvdseagp evaqfseeeh
      481 raaavyfked ldflqkvgss qrlgrtslgr cslflnfdpl lansptnaps eesrpknrys
      541 lrsqlsekgs sladlsesed llnltapspp hgasvfveeq mfsekemsqa lkyqelmfqe
      601 rllkkdqefv rqlesarqea esfrgklees qelfqiqera lqclerhten lvealqlvsk
      661 daqkesaeke eslqnvarer dqlledlhcm ektfsdfhrr yekakeairt lkqneailkk
      721 qlaeageale knchmvhiik tkteesiega nkeventkrs feaditvlkg qlkkatmtin
      781 slekrleqkn eenaeltkly ddllnqvkak
//
LOCUS       XP_077503192             810 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113759 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503192
VERSION     XP_077503192.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647066.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..810
                     /product="uncharacterized protein LOC144113759 isoform X2"
                     /calculated_mol_wt=88040
     Region          <109..232
                     /region_name="PRK10856"
                     /note="cytoskeleton protein RodZ"
                     /db_xref="CDD:236776"
     Region          582..804
                     /region_name="TACC_C"
                     /note="Transforming acidic coiled-coil-containing protein
                     (TACC), C-terminal; pfam05010"
                     /db_xref="CDD:461517"
     CDS             1..810
                     /gene="LOC144113759"
                     /coded_by="XM_077647066.1:109..2541"
                     /db_xref="GeneID:144113759"
ORIGIN      
        1 mgnqpgvarm dtsapdgkps tsrrksteke qrlsvgassd pqesliaara ilsdivnhvv
       61 sersprptea sadngnsvln rtlssrslks yllagqcvpd edcanrtfpl eadpegserp
      121 sptsanagss gpadtgvvte psgsaradna lrpctseasa easpcvllsp enrssdahpv
      181 asrsatpvdt dqggsasdsv seppssdcwy ytdasdaslp gvgankseld ltyttakgsp
      241 lgaarffdts slhtsnvaps edtevnhpag safnescdqe eafcdsqpad alelhdghle
      301 tsgnvtcdvl rlqdsesspe stlkllspgl iektfespsk nfgvlapsdl qkastpttqe
      361 dvvphfdyhc svqsptdfcn ktckaseass hletpththr qdetvtlsat gfnenllsss
      421 dpsnaevtvi ekdpatpaav lgneatahiq chpdkgtteg tlpvdseagp evaqfseeeh
      481 raaavyfked ldflqkvgss qrlgrtslgr cslflnfdpl lansptnaps eesrpknrys
      541 lrsqlsekgs sladlsesed llnltapspp hgasvfveeq mfsekemsqa lkyqelmfqe
      601 rllkkdqefv rqlesarqea esfrgklees qelfqiqera lqclerhten lvealqlvsk
      661 daqkesaeke eslqnvarer dqlledlhcm ektfsdfhrr yekakeairt lkqneailkk
      721 qlaeageale knchmvhiik tkteesiega nkeventkrs feaditvlkg qlkkatmtin
      781 slekrleqkn eenaeltkly ddllnqvkak
//
LOCUS       XP_077503193             809 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113759 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503193
VERSION     XP_077503193.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647067.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..809
                     /product="uncharacterized protein LOC144113759 isoform X3"
                     /calculated_mol_wt=88052
     Region          <109..232
                     /region_name="PRK10856"
                     /note="cytoskeleton protein RodZ"
                     /db_xref="CDD:236776"
     Region          581..803
                     /region_name="TACC_C"
                     /note="Transforming acidic coiled-coil-containing protein
                     (TACC), C-terminal; pfam05010"
                     /db_xref="CDD:461517"
     CDS             1..809
                     /gene="LOC144113759"
                     /coded_by="XM_077647067.1:146..2575"
                     /db_xref="GeneID:144113759"
ORIGIN      
        1 middpssekk enlppdgkps tsrrksteke qrlsvgassd pqesliaara ilsdivnhvv
       61 sersprptea sadngnsvln rtlssrslks yllagqcvpd edcanrtfpl eadpegserp
      121 sptsanagss gpadtgvvte psgsaradna lrpctseasa easpcvllsp enrssdahpv
      181 asrsatpvdt dqggsasdsv seppssdcwy ytdasdaslp gvgankseld ltyttakgsp
      241 lgaarffdts slhtsnvaps edtevnhpag safnescdqe eafcdsqpad alelhdghle
      301 tsgnvtcdvl rlqdsesspe stlkllspgl iektfespsk nfgvlapsdl qkastpttqe
      361 dvvphfdyhc svqsptdfcn ktckaseass hletpththr qdetvtlsat gfnenllsss
      421 dpsnaevtvi ekdpatpaav lgneatahiq chpdkgtteg tlpvdseagp evaqfseeeh
      481 raaavyfked ldflqkvgss qrlgrtslgr cslflnfdpl lansptnaps eesrpknrys
      541 lsqlsekgss ladlsesedl lnltapspph gasvfveeqm fsekemsqal kyqelmfqer
      601 llkkdqefvr qlesarqeae sfrgkleesq elfqiqeral qclerhtenl vealqlvskd
      661 aqkesaekee slqnvarerd qlledlhcme ktfsdfhrry ekakeairtl kqneailkkq
      721 laeagealek nchmvhiikt kteesiegan keventkrsf eaditvlkgq lkkatmtins
      781 lekrleqkne enaeltklyd dllnqvkak
//
LOCUS       XP_077503194             725 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113761 [Amblyomma americanum].
ACCESSION   XP_077503194
VERSION     XP_077503194.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647068.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..725
                     /product="uncharacterized protein LOC144113761"
                     /calculated_mol_wt=80708
     Region          374..393
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(379,381,383,385..386,388..389,392,406,408,412..413,
                     416..417,420,434,436,438,440..441,444..445)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          401..421
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          429..445
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          508..528
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(513,515,517,519..520,523..524,527,542,547..548,
                     551..552,555,569,571,573,575..576,579..580)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          533..>606
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          536..556
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          564..581
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          595..612
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..725
                     /gene="LOC144113761"
                     /coded_by="XM_077647068.1:138..2315"
                     /db_xref="GeneID:144113761"
ORIGIN      
        1 mfdneeddth lcldvktyia hrkqlqhcck ivrndsalls vvsptaehqy epssqraddf
       61 fsslelqsiq taipneshgc csaskvgqad edledaedsd ndlypptsht ggkwkpgwgp
      121 parfdwkava qhrevplqav kepaasseef icvpcnrrys nnfsylrhke twyhlkrsgc
      181 istvevkssk rrlalvaagt avqeegqkca gtnslchasd vgtescckrp ssiqedmqqq
      241 rssspaaavq psysssncic sdnevlnkek klptgkcpdv iqpqangsvq ntvpvqgqga
      301 elccvtcnaq fltvnefrlh qchftvagqa ngkflystel setaltnips dgprlltkda
      361 smqrkhtsvp klpcphckkl fsksymklhm hrhtgekpfv csicdqhfsh rstlnihlkh
      421 hsglrkfhcn qcnfravrrs mlrrhelsvh rptvdlqpcp spvansllqq qgvqqkshnv
      481 snqkafseak isacrtqslp kpqpyficds csykttkshl lldhqrlhtg eqpfqcpqcn
      541 fkasrrsqmh rhsrihlglk pyscpyckyr cnnqenlrkh ilntkkhsgk qmypcsqcnf
      601 acneykaykn hvsdkhqkdl tnnsdsltvt agvenqetll pkccdeitdp pstdkpmtds
      661 cttlpepavf ltiisglsep dleysgatsq alavldgltc adnevlaess sgillpssld
      721 selsd
//
LOCUS       XP_077503195             293 aa            linear   INV 25-NOV-2025
DEFINITION  serine hydrolase BPHL isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503195
VERSION     XP_077503195.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647069.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..293
                     /product="serine hydrolase BPHL isoform X1"
                     /calculated_mol_wt=33072
     Region          40..291
                     /region_name="MenH"
                     /note="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase MenH and related
                     esterases, alpha/beta hydrolase fold [Coenzyme transport
                     and metabolism, General function prediction only];
                     COG0596"
                     /db_xref="CDD:440361"
     CDS             1..293
                     /gene="LOC144113762"
                     /coded_by="XM_077647069.1:153..1034"
                     /db_xref="GeneID:144113762"
ORIGIN      
        1 mntlsafakt aplrpllklq facsfvrcss tlkpasalnp pgfyvealny rihvetvgng
       61 prpvvllpga igstrtdfal qlekldrklf tiiawdppgy gfsrppernf pvdfyhrdar
      121 vlakvmhklg hkqysvigws dgantgmila aiqpkcvqkl vafggnayit eqdvqlleat
      181 rniekwsekm rasmeelygt ehfrhlwsny cdfyqdllqk hqgevcrsel phircptliv
      241 hggkdplvps hhahyllqhi anaklyvipd gkhnlhlkya defnkivtef lis
//
LOCUS       XP_077503196             239 aa            linear   INV 25-NOV-2025
DEFINITION  serine hydrolase BPHL isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503196
VERSION     XP_077503196.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647070.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..239
                     /product="serine hydrolase BPHL isoform X2"
                     /calculated_mol_wt=27286
     Region          18..237
                     /region_name="MenH"
                     /note="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase MenH and related
                     esterases, alpha/beta hydrolase fold [Coenzyme transport
                     and metabolism, General function prediction only];
                     COG0596"
                     /db_xref="CDD:440361"
     CDS             1..239
                     /gene="LOC144113762"
                     /coded_by="XM_077647070.1:171..890"
                     /db_xref="GeneID:144113762"
ORIGIN      
        1 malglatkhq rhsftaagst rtdfalqlek ldrklftiia wdppgygfsr ppernfpvdf
       61 yhrdarvlak vmhklghkqy svigwsdgan tgmilaaiqp kcvqklvafg gnayiteqdv
      121 qlleatrnie kwsekmrasm eelygtehfr hlwsnycdfy qdllqkhqge vcrselphir
      181 cptlivhggk dplvpshhah yllqhianak lyvipdgkhn lhlkyadefn kivteflis
//
LOCUS       XP_077503198             374 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like [Amblyomma americanum].
ACCESSION   XP_077503198
VERSION     XP_077503198.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647072.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..374
                     /product="tubulin beta chain-like"
                     /calculated_mol_wt=41888
     Region          1..358
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..374
                     /gene="LOC144113763"
                     /coded_by="XM_077647072.1:58..1182"
                     /db_xref="GeneID:144113763"
ORIGIN      
        1 mdsvrsgpfg qlfrpdnfif gqsgagnnwa kghytegael vdsvldvvrk eaescdclqg
       61 fqlahslggg tgsgmgtlli skireeypdr imntfsvmps pkvsdtvvep ynatlsvhql
      121 ventdetyci dnealydicf rtlklttpty gdlnhlvsvt msgvttclrf pgqlnadlrk
      181 lavnmvpfpr lhffmpgfap ltsrgsqqyr altvpeltqq mfdaknmmaa cdprhgrylt
      241 vatifrgrmr mkevdeqmln vqnknssyfv ewipnnvkta vcdipprglk msatfignst
      301 aiqelfkris eqftamfrrk aflhwytgeg mdemefteae snmndlvsey qqyqeatadd
      361 egefddedal adta
//
LOCUS       XP_077503199              59 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like [Amblyomma americanum].
ACCESSION   XP_077503199
VERSION     XP_077503199.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647073.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..59
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..59
                     /product="tubulin beta chain-like"
                     /calculated_mol_wt=6764
     Region          <1..39
                     /region_name="Tubulin_FtsZ_Cetz-like"
                     /note="Tubulin protein family of FtsZ and CetZ-like;
                     cl10017"
                     /db_xref="CDD:471962"
     CDS             1..59
                     /gene="LOC144113764"
                     /coded_by="XM_077647073.1:6..185"
                     /db_xref="GeneID:144113764"
ORIGIN      
        1 mfrrkaflhw ytgegmdeme fteaesnmnd lvseyqqyqe ataddegefd dedaladta
//
LOCUS       XP_077503200            1001 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113765 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503200
VERSION     XP_077503200.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647074.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1001
                     /product="uncharacterized protein LOC144113765 isoform X1"
                     /calculated_mol_wt=112718
     Region          58..134
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          339..>481
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..1001
                     /gene="LOC144113765"
                     /coded_by="XM_077647074.1:109..3114"
                     /db_xref="GeneID:144113765"
ORIGIN      
        1 mtqrvcaasa aagnaaaqwq qttatgasia klrerlptrr ahwtsvitvl svmshmycca
       61 kwcstsgksg ehlfrlpsdh rfsiwlkyan rlelletpre kvyktyrlcs khftrdcfts
      121 dactrlthea vpsvkvhepr lralvhpdfy eggaaqgies asplqstaap vevmlghdyl
      181 plpstlglgt iagadsavhd tddphssrap qltdvqttpp ppadeegaaq stpirviasa
      241 swdspdyaea gpsglsgake svrqpvmtpa sgtpksrrrk gavvytprtk krfeklhsrs
      301 dryrkalsri rkrhdtrrvt qqealnklep hlpqdlfelv kaqirlcsfp kskkhwskqi
      361 kqfalnlffh spkayrflre klhlphvrtl rrwladipmt pgiipgamda iesitkdwpl
      421 qdkaccllfd eislkknlmy dqskdivigf tddgntrtsr vanialvmav sgisrswmqp
      481 vafaashnat svlamrklll dliqqlqtkg llvkavicdq gsnnvslsrl lihsihepyf
      541 vvnnrkiyfm fdtphlikct rnnlrrhklt igsevvdwth ietlykktrh idrasktpve
      601 hlksrlakkl keqhiyrmpf admrvkyatq vfsesvsval ltliaiqelp vdakftaeft
      661 ermdklfdcl nssalkkqdd klryamtegs ehhvflesci dwiaqwhfgg pldkqphtik
      721 gwqitikall llwtdlkadf dythlftrrl qqdplenffa ivrqkhgcne tpnvyqfvaa
      781 vkhiwigqlf klsqgncevt thafltnles vsavlspsst pstshsrqqg sgssdlqass
      841 qdapdivyen vvyhvagtlv ksfldqrtte ctcegtllaa dggslhgrhq ffallkengv
      901 pehlfqsiaa tseaclnyik kldsafrkei sccahlsnvs rnlvermpnq qavfcslgck
      961 ikflklfcrt rlhwhlsfln rsmtsktkqs aparkrrkla g
//
LOCUS       XP_077503201             828 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113765 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503201
VERSION     XP_077503201.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647075.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..828
                     /product="uncharacterized protein LOC144113765 isoform X2"
                     /calculated_mol_wt=93471
     Region          166..>308
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..828
                     /gene="LOC144113765"
                     /coded_by="XM_077647075.1:665..3151"
                     /db_xref="GeneID:144113765"
ORIGIN      
        1 mlghdylplp stlglgtiag adsavhdtdd phssrapqlt dvqttppppa deegaaqstp
       61 irviasaswd spdyaeagps glsgakesvr qpvmtpasgt pksrrrkgav vytprtkkrf
      121 eklhsrsdry rkalsrirkr hdtrrvtqqe alnklephlp qdlfelvkaq irlcsfpksk
      181 khwskqikqf alnlffhspk ayrflreklh lphvrtlrrw ladipmtpgi ipgamdaies
      241 itkdwplqdk accllfdeis lkknlmydqs kdivigftdd gntrtsrvan ialvmavsgi
      301 srswmqpvaf aashnatsvl amrkllldli qqlqtkgllv kavicdqgsn nvslsrllih
      361 sihepyfvvn nrkiyfmfdt phlikctrnn lrrhkltigs evvdwthiet lykktrhidr
      421 asktpvehlk srlakklkeq hiyrmpfadm rvkyatqvfs esvsvalltl iaiqelpvda
      481 kftaefterm dklfdclnss alkkqddklr yamtegsehh vflescidwi aqwhfggpld
      541 kqphtikgwq itikallllw tdlkadfdyt hlftrrlqqd plenffaivr qkhgcnetpn
      601 vyqfvaavkh iwigqlfkls qgncevttha fltnlesvsa vlspsstpst shsrqqgsgs
      661 sdlqassqda pdivyenvvy hvagtlvksf ldqrttectc egtllaadgg slhgrhqffa
      721 llkengvpeh lfqsiaatse aclnyikkld safrkeiscc ahlsnvsrnl vermpnqqav
      781 fcslgckikf lklfcrtrlh whlsflnrsm tsktkqsapa rkrrklag
//
LOCUS       XP_077503202             231 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113765 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503202
VERSION     XP_077503202.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647076.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..231
                     /product="uncharacterized protein LOC144113765 isoform X3"
                     /calculated_mol_wt=25198
     Region          58..134
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..231
                     /gene="LOC144113765"
                     /coded_by="XM_077647076.1:109..804"
                     /db_xref="GeneID:144113765"
ORIGIN      
        1 mtqrvcaasa aagnaaaqwq qttatgasia klrerlptrr ahwtsvitvl svmshmycca
       61 kwcstsgksg ehlfrlpsdh rfsiwlkyan rlelletpre kvyktyrlcs khftrdcfts
      121 dactrlthea vpsvkvhepr lralvhpdfy eggaaqgies asplqstaap vevmlghdyl
      181 plpstlglgt iagadsavhd tddphssrap qltdvqttpp ppadeevclf i
//
LOCUS       XP_077503203             374 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain-like [Amblyomma americanum].
ACCESSION   XP_077503203
VERSION     XP_077503203.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647077.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..374
                     /product="tubulin beta chain-like"
                     /calculated_mol_wt=41819
     Region          1..358
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..374
                     /gene="LOC144113766"
                     /coded_by="XM_077647077.1:169..1293"
                     /db_xref="GeneID:144113766"
ORIGIN      
        1 mdsvrsgpfg qlfrpdnfif gqsgagnnwa kghytegael vdsvldvvrk eaescdclqg
       61 fqlahslggg tgsgmgtlli skireeypdr imntfsvmps pkvsdtvvep ynatlsvhql
      121 ventdetyci dnealydicf rtlklttpty gdlnhlvsvt msgvttclrf pgqlnadlrk
      181 lavnmvpfpr lhffmpgfap ltsrgsqqyr altvpeltqq mfdaknmmaa cdprhgrylt
      241 vatifrgrms mkevdeqmln vqnknssyfv ewipnnvkta vcdipprglk msatfignst
      301 aiqelfkris eqftamfrrk aflhwytgeg mdemefteae snmndlvsey qqyqeatadd
      361 egefddedal adta
//
LOCUS       XP_077503204             357 aa            linear   INV 25-NOV-2025
DEFINITION  methylthioribose-1-phosphate isomerase-like [Amblyomma americanum].
ACCESSION   XP_077503204
VERSION     XP_077503204.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647078.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..357
                     /product="methylthioribose-1-phosphate isomerase-like"
                     /calculated_mol_wt=37986
     Region          3..346
                     /region_name="salvage_mtnA"
                     /note="S-methyl-5-thioribose-1-phosphate isomerase;
                     TIGR00512"
                     /db_xref="CDD:273112"
     CDS             1..357
                     /gene="LOC144113768"
                     /coded_by="XM_077647078.1:190..1263"
                     /db_xref="GeneID:144113768"
ORIGIN      
        1 mlqsirykag tleildqrrl pheihyltla dakdvseair fmkvqgapai amvaalgvav
       61 eltwraqlps sradlaalvh flldyistsr praanlayva aqlgsmadtl aeyeglsvea
      121 mrdaicaeae vmlaadlafn rligqygaeh vvancraaag dgklgvlthc nagtlatagy
      181 gtalgvvrrl hesgrleray ctetrpcnqg srltayellh dripatvicd smvaallatg
      241 rvhavvvgad rvtangdtan tigtyqlaic akhhgipfyv asptttvdvr vltgkdvlie
      301 erparemtnv qgielapngt ewwnpaldvt paelitggii tqkgvfpasr vldslss
//
LOCUS       XP_077503205             357 aa            linear   INV 25-NOV-2025
DEFINITION  methylthioribose-1-phosphate isomerase-like [Amblyomma americanum].
ACCESSION   XP_077503205
VERSION     XP_077503205.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647079.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..357
                     /product="methylthioribose-1-phosphate isomerase-like"
                     /calculated_mol_wt=37986
     Region          3..346
                     /region_name="salvage_mtnA"
                     /note="S-methyl-5-thioribose-1-phosphate isomerase;
                     TIGR00512"
                     /db_xref="CDD:273112"
     CDS             1..357
                     /gene="LOC144113768"
                     /coded_by="XM_077647079.1:172..1245"
                     /db_xref="GeneID:144113768"
ORIGIN      
        1 mlqsirykag tleildqrrl pheihyltla dakdvseair fmkvqgapai amvaalgvav
       61 eltwraqlps sradlaalvh flldyistsr praanlayva aqlgsmadtl aeyeglsvea
      121 mrdaicaeae vmlaadlafn rligqygaeh vvancraaag dgklgvlthc nagtlatagy
      181 gtalgvvrrl hesgrleray ctetrpcnqg srltayellh dripatvicd smvaallatg
      241 rvhavvvgad rvtangdtan tigtyqlaic akhhgipfyv asptttvdvr vltgkdvlie
      301 erparemtnv qgielapngt ewwnpaldvt paelitggii tqkgvfpasr vldslss
//
LOCUS       XP_077503206             276 aa            linear   INV 25-NOV-2025
DEFINITION  18S rRNA (guanine-N(7))-methyltransferase [Amblyomma americanum].
ACCESSION   XP_077503206
VERSION     XP_077503206.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647080.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..276
                     /product="18S rRNA (guanine-N(7))-methyltransferase"
                     /calculated_mol_wt=30423
     Region          38..181
                     /region_name="UbiE"
                     /note="Ubiquinone/menaquinone biosynthesis C-methylase
                     UbiE/MenG [Coenzyme transport and metabolism]; COG2226"
                     /db_xref="CDD:441828"
     Region          201..272
                     /region_name="WBS_methylT"
                     /note="Methyltransferase involved in Williams-Beuren
                     syndrome; pfam12589"
                     /db_xref="CDD:432656"
     CDS             1..276
                     /gene="LOC144113770"
                     /coded_by="XM_077647080.1:125..955"
                     /db_xref="GeneID:144113770"
ORIGIN      
        1 msrrpeysap pevfyseqea akytsntrim eiqsamsera vellalpdde tclildlgcg
       61 sglsgdviee qghvwvgvdi sramlnvaqe reaagdmllg dlgqglcfra gafdgaisis
      121 alqwlcnadk kshnpvkrlh affsslyacl grgrravlqf ypenadqvel ltqqamragf
      181 tgglvvdypn stkakkmflv lftggpqklp talgtsaeqq dqiectarkq rlkklrgkgp
      241 ksaaawiqek kerrrrqgke trpdskytgr krcgkf
//
LOCUS       XP_077503207             409 aa            linear   INV 25-NOV-2025
DEFINITION  GTPase Era, mitochondrial [Amblyomma americanum].
ACCESSION   XP_077503207
VERSION     XP_077503207.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647081.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..409
                     /product="GTPase Era, mitochondrial"
                     /calculated_mol_wt=45552
     Region          68..404
                     /region_name="era"
                     /note="GTPase Era; Reviewed; PRK00089"
                     /db_xref="CDD:234624"
     CDS             1..409
                     /gene="LOC144113771"
                     /coded_by="XM_077647081.1:233..1462"
                     /db_xref="GeneID:144113771"
ORIGIN      
        1 mfssrccvpv rltaltrsil clspgpqlts aidfpraayr tvprtlaeyn hlksivplqp
       61 cnpkllrvav vgqpnvgkst lvntlmrwkv csvsskvhtt rqnakailvd getqivfldt
      121 pgivdidhgm khhleasmiv dpehallnad lvavmvdasd hwrrhsfdre tlrllefnah
      181 ltsilvvnkv dllkskrqil efthsltggi vggvptcrsr tvkrrltpee lfsrtdemlr
      241 tgdlnleget vglrvdhlkq tqgnsrnswp nfsrifmisa lkndgvkdik dfllqsarps
      301 ewmypfemvt dqnphelatl ivrekilnhl srevpynvea kvamwdldaa gvlhivieil
      361 gknrrhvkhi lgeggktvka vaaesrqdia aafrsdvslk livknssag
//
LOCUS       XP_077503208             446 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503208
VERSION     XP_077503208.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647082.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..446
                     /product="tubulin beta chain isoform X1"
                     /calculated_mol_wt=49784
     Region          1..430
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..446
                     /gene="LOC144113772"
                     /coded_by="XM_077647082.1:100..1440"
                     /db_xref="GeneID:144113772"
ORIGIN      
        1 mreivhiqag qcgnqigakf wevisdehgi dptgtyhgds dlqlerinvy yneasggkyv
       61 prailvdlep gtmdsvrsgp fgqlfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaegcdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntfsvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmpgf apltsrgsqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevdeqm lnvqnknssy fvewipnnvk tavcdipprg
      361 lkmsatfign staiqelfkr iseqftamfr rkaflhwytg egmdemefte aesnmndlvs
      421 eyqqyqeata deegefedee eaqaea
//
LOCUS       XP_077503209             445 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta chain isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503209
VERSION     XP_077503209.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647083.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..445
                     /product="tubulin beta chain isoform X2"
                     /calculated_mol_wt=49727
     Region          1..429
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..445
                     /gene="LOC144113772"
                     /coded_by="XM_077647083.1:100..1437"
                     /db_xref="GeneID:144113772"
ORIGIN      
        1 mreivhiqag qcgnqigakf wevisdehgi dptgtyhgds dlqlerinvy yneasgkyvp
       61 railvdlepg tmdsvrsgpf gqlfrpdnfv fgqsgagnnw akghytegae lvdsvldvvr
      121 keaegcdclq gfqlthslgg gtgsgmgtll iskireeypd rimntfsvvp spkvsdtvve
      181 pynatlsvhq lventdetyc idnealydic frtlklttpt ygdlnhlvsa tmsgvttclr
      241 fpgqlnadlr klavnmvpfp rlhffmpgfa pltsrgsqqy raltvpeltq qmfdaknmma
      301 acdprhgryl tvaavfrgrm smrevdeqml nvqnknssyf vewipnnvkt avcdipprgl
      361 kmsatfigns taiqelfkri seqftamfrr kaflhwytge gmdemeftea esnmndlvse
      421 yqqyqeatad eegefedeee aqaea
//
LOCUS       XP_077503210             190 aa            linear   INV 25-NOV-2025
DEFINITION  NEDD8-conjugating enzyme UBE2F-like [Amblyomma americanum].
ACCESSION   XP_077503210
VERSION     XP_077503210.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647084.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..190
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..190
                     /product="NEDD8-conjugating enzyme UBE2F-like"
                     /calculated_mol_wt=21653
     Region          36..182
                     /region_name="UBCc_UBE2F_UBE2M"
                     /note="Ubiquitin-conjugating enzyme E2, catalytic (UBCc)
                     domain of ubiquitin conjugating enzymes E2 F, E2 M and
                     related proteins; cd23794"
                     /db_xref="CDD:467414"
     CDS             1..190
                     /gene="LOC144113773"
                     /coded_by="XM_077647084.1:156..728"
                     /db_xref="GeneID:144113773"
ORIGIN      
        1 mitlskklkq ssqnnntdsv qslvpesvga vrrasvrdkl lvrevqemea nlpntctlkf
       61 ddpdvlhrfe liiapdegyw qggrfvflvd vpedynmvpp kvkcttrlwh pnineegnvc
      121 lsllressid gmgwaptrtl keivwglnal ftdllnfddp ldneaaehyq rdkegfvakv
      181 reyvqkyakr
//
LOCUS       XP_077503211             300 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113774 [Amblyomma americanum].
ACCESSION   XP_077503211
VERSION     XP_077503211.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647085.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..300
                     /product="uncharacterized protein LOC144113774"
                     /calculated_mol_wt=32437
     Region          75..150
                     /region_name="THAP"
                     /note="The THAP domain is a putative DNA-binding domain
                     (DBD) and probably also binds a zinc ion; smart00980"
                     /db_xref="CDD:214951"
     CDS             1..300
                     /gene="LOC144113774"
                     /coded_by="XM_077647085.1:1..903"
                     /db_xref="GeneID:144113774"
ORIGIN      
        1 mlllrsfrrp ctsllchrsv rfaprctaaa vfsvlsfpsa cgqcevffrl ctlralllkr
       61 lalhfkmslr hvkkrcsvvh cdsvdstigv tlhrfpldfh rcrawvqfcr nvslekkaps
      121 qlrelricsr hfepsvylts grlrhdalpt rasiadtsse cssdmncsqs lsetsasvrr
      181 agepaavsge pvskgyvqsr ssqslllfqe dadgasptgs tlnqacshas gfimvqsdht
      241 yagpsteasa sslepgtagv aatlsastdl spgapfsssp lsyegsftsp inerrplqkl
//
LOCUS       XP_077503212             224 aa            linear   INV 25-NOV-2025
DEFINITION  methionine-R-sulfoxide reductase B2, mitochondrial-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077503212
VERSION     XP_077503212.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647086.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..224
                     /product="methionine-R-sulfoxide reductase B2,
                     mitochondrial-like isoform X1"
                     /calculated_mol_wt=25448
     Region          62..>159
                     /region_name="MsrB"
                     /note="Peptide methionine sulfoxide reductase MsrB
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG0229"
                     /db_xref="CDD:439999"
     CDS             1..224
                     /gene="LOC144113775"
                     /coded_by="XM_077647086.1:183..857"
                     /db_xref="GeneID:144113775"
ORIGIN      
        1 msvlwgarni vkkpffsrlc eclfraataq airppnnkpv vraihlsdlf rmadqkdvkd
       61 mtekdwrekl tpdqfyicrq rgtepayrgr wvnhkakgty tciacgadlf ssehkfvsnc
      121 gwpsffmalg tvdgdeshss iirkedsshg mirtevcckk mlitwttsrl llcakkkekr
      181 lppvafsvht mlhlkqhrac atltwgtcsm mdhsqhdfaf asta
//
LOCUS       XP_077503213             195 aa            linear   INV 25-NOV-2025
DEFINITION  methionine-R-sulfoxide reductase B2, mitochondrial-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077503213
VERSION     XP_077503213.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647087.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..195
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..195
                     /product="methionine-R-sulfoxide reductase B2,
                     mitochondrial-like isoform X2"
                     /calculated_mol_wt=21505
     Region          62..>159
                     /region_name="MsrB"
                     /note="Peptide methionine sulfoxide reductase MsrB
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG0229"
                     /db_xref="CDD:439999"
     CDS             1..195
                     /gene="LOC144113775"
                     /coded_by="XM_077647087.1:183..770"
                     /db_xref="GeneID:144113775"
ORIGIN      
        1 msvlwgarni vkkpffsrlc eclfraataq airppnnkpv vraihlsdlf rmadqkdvkd
       61 mtekdwrekl tpdqfyicrq rgtepayrgr wvnhkakgty tciacgadlf ssehkfvsnc
      121 gwpsffmalg tvdgdeshss iirkedsshg mirtevcckk vagsphtsag iavqqlshas
      181 llqqvaisnt atgga
//
LOCUS       XP_077503214             194 aa            linear   INV 25-NOV-2025
DEFINITION  peptide methionine sulfoxide reductase MsrB-like isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077503214
VERSION     XP_077503214.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647088.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..194
                     /product="peptide methionine sulfoxide reductase MsrB-like
                     isoform X3"
                     /calculated_mol_wt=21967
     Region          62..194
                     /region_name="MsrB"
                     /note="Peptide methionine sulfoxide reductase MsrB
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG0229"
                     /db_xref="CDD:439999"
     CDS             1..194
                     /gene="LOC144113775"
                     /coded_by="XM_077647088.1:183..767"
                     /db_xref="GeneID:144113775"
ORIGIN      
        1 msvlwgarni vkkpffsrlc eclfraataq airppnnkpv vraihlsdlf rmadqkdvkd
       61 mtekdwrekl tpdqfyicrq rgtepayrgr wvnhkakgty tciacgadlf ssehkfvsnc
      121 gwpsffmalg tvdgdeshss iirkedsshg mirtevcckk cnshlghvfn dgplptrlrf
      181 cinsialnfr papk
//
LOCUS       XP_077503215             166 aa            linear   INV 25-NOV-2025
DEFINITION  peptide methionine sulfoxide reductase MsrB-like isoform X4
            [Amblyomma americanum].
ACCESSION   XP_077503215
VERSION     XP_077503215.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647089.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..166
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..166
                     /product="peptide methionine sulfoxide reductase MsrB-like
                     isoform X4"
                     /calculated_mol_wt=18807
     Region          62..>159
                     /region_name="MsrB"
                     /note="Peptide methionine sulfoxide reductase MsrB
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG0229"
                     /db_xref="CDD:439999"
     CDS             1..166
                     /gene="LOC144113775"
                     /coded_by="XM_077647089.1:183..683"
                     /db_xref="GeneID:144113775"
ORIGIN      
        1 msvlwgarni vkkpffsrlc eclfraataq airppnnkpv vraihlsdlf rmadqkdvkd
       61 mtekdwrekl tpdqfyicrq rgtepayrgr wvnhkakgty tciacgadlf ssehkfvsnc
      121 gwpsffmalg tvdgdeshss iirkedsshg mirtevcckk nlmpta
//
LOCUS       XP_077503217             445 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113780 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503217
VERSION     XP_077503217.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647091.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..445
                     /product="uncharacterized protein LOC144113780 isoform X1"
                     /calculated_mol_wt=47992
     CDS             1..445
                     /gene="LOC144113780"
                     /coded_by="XM_077647091.1:123..1460"
                     /db_xref="GeneID:144113780"
ORIGIN      
        1 msesrtnvls lrdadneals aegnpplgdv avdhgpppag slvrsssawm gvnwplqnws
       61 tarrghsdgh viprdaatst scenvavptr qatvkrkrvv dvgqktvkqm nelaryvnak
      121 gpcaicktpg avvtltqikr rrrkfmryws kqggpeeskg spdvwppdst pllvtatksq
      181 ksvswiehhe vqrdvgpdfm nsetgaflll lsvvalvvgi lvivkatens lkeylglitp
      241 tvslssalpv gseelsveqg rpstedvpaq pagdqdelrl aqeaaarqiq rwyrrcqgsp
      301 ragldadgar rkrvdrwaqs lrsffsrkki nhiragfqkr wgkkradrva segaddlapl
      361 eanevltgag svhsvcdgss pqpsagsqqd aavvsstsna gslasrsrlf pnggvqgvet
      421 qrsstcllqr srdenpvigr srsaq
//
LOCUS       XP_077503218             360 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113780 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503218
VERSION     XP_077503218.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647092.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..360
                     /product="uncharacterized protein LOC144113780 isoform X2"
                     /calculated_mol_wt=38292
     CDS             1..360
                     /gene="LOC144113780"
                     /coded_by="XM_077647092.1:123..1205"
                     /db_xref="GeneID:144113780"
ORIGIN      
        1 msesrtnvls lrdadneals aegnpplgdv avdhgpppag slvrsssawm gvnwplqnws
       61 tarrghsdgh viprdaatst scenvavptr qatvkrkrvv dvgqktvkqm nelaryvnak
      121 gpcaicktpg avvtltqikr rrrkfmryws kqggpeeskg spdvwppdst pllvtatksq
      181 ksvswiehhe vqrdvgpdfm nsetgaflll lsvvalvvgi lvivkatens lkeylglitp
      241 tvslssalpa gfqkrwgkkr adrvasegad dlapleanev ltgagsvhsv cdgsspqpsa
      301 gsqqdaavvs stsnagslas rsrlfpnggv qgvetqrsst cllqrsrden pvigrsrsaq
//
LOCUS       XP_077503219             211 aa            linear   INV 25-NOV-2025
DEFINITION  lysozyme 2-like [Amblyomma americanum].
ACCESSION   XP_077503219
VERSION     XP_077503219.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647093.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..211
                     /product="lysozyme 2-like"
                     /calculated_mol_wt=23643
     Region          72..193
                     /region_name="LYZ_C_invert"
                     /note="C-type invertebrate lysozyme; cd16899"
                     /db_xref="CDD:381618"
     CDS             1..211
                     /gene="LOC144113781"
                     /coded_by="XM_077647093.1:460..1095"
                     /db_xref="GeneID:144113781"
ORIGIN      
        1 mppckqpadr aplghrwpga ragsarmdet srpqqqdgfg arlrrlsakw lqmlfhaall
       61 aalvalpcsw gkvfdkcela relyfvhgfp edqlaqwvci aeheslfnss avghmnwdgs
      121 adhglfqind rywcsppgpk necridcadl eddnitddve carliykrhg fsawavwrgl
      181 crgvnfsnyl secvsgnavh vnsvvytygr k
//
LOCUS       XP_077503220             910 aa            linear   INV 25-NOV-2025
DEFINITION  exocyst complex component secretory 5 [Amblyomma americanum].
ACCESSION   XP_077503220
VERSION     XP_077503220.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647094.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..910
                     /product="exocyst complex component secretory 5"
                     /calculated_mol_wt=102878
     Region          5..85
                     /region_name="IPT_PCSR"
                     /note="IPT domain of Plexins and Cell Surface Receptors
                     (PCSR) and related proteins . This subgroup contains IPT
                     domains of plexins, receptors, like the
                     plasminogen-related growth factor receptors, the
                     hepatocyte growth factor-scatter factors, and the...;
                     cd00603"
                     /db_xref="CDD:238337"
     Region          128..902
                     /region_name="Sec5"
                     /note="Exocyst complex component Sec5; pfam15469"
                     /db_xref="CDD:464733"
     CDS             1..910
                     /gene="Sec5"
                     /coded_by="XM_077647094.1:253..2985"
                     /db_xref="GeneID:144113783"
ORIGIN      
        1 maeppkvtgl splegppgtk vtirgenlgl dqndllnvti cgvdcsmyse wkssskivtr
       61 trvctgrgev lvttrsggvg tctvnfrgil evigptvesa iwvdeshmfs dvfgrkghpt
      121 sptqlhpedp lgisdesvkr fpeeelrqlf pegsgnllse nfnptwylle nynstsfndl
      181 kaglqslkrk vgqmqegpss flksnvnaiv icldvlhdlq kamekdkqem gadlteklfe
      241 avtrskneae nifesilark dqadstrnal gilqkfrflf nlpvtiekni qkgdydivin
      301 dynrakslfq ntevavfrrv fndveqrvqr frnalmdnlk qlpmpvdeqk rlirylisle
      361 vpgspacdai rhnyswlfds vmackkrhlt mlaideeyeq fgksgdesqp kqvlfveevt
      421 elfqkhlpdf wklgqayfag dlfvlenvsv npkdekdfkd mlgrlvaklc wevrlallpp
      481 thrslvgsgl gqesdggkcn mwpvhtqger lgpwlpqclr sirqcylsll kleipeenta
      541 qiqqlifdlr vhsmtclfgq asedvrnllk sevwrleqdd rcggttqlpl mfenrvietl
      601 qlvrenilvs gpkemevfeq invqgaikql aqgvllafve tlekcalspd rlspmidsig
      661 gpvslsrspv evtpssqrak gwkqkredsl ttpplekrll ivlsncsfts rvviprlqes
      721 fkrhgypdms pvikiaqsrl vdldnklflk lleakcdpiv gaiepsmyag afdwkrcpep
      781 iavspyikev lmnlievhae vytispplvg rimtplaqsv aeeiariyec tekftkygnm
      841 qatldlrale eavdfyrnqs tgnyfmicrs kldpfgntre kelvdqllqk frtqmklqll
      901 cfkedvvvsv
//
LOCUS       XP_077503221             831 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin carboxyl-terminal hydrolase 10-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503221
VERSION     XP_077503221.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647095.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..831
                     /product="ubiquitin carboxyl-terminal hydrolase 10-like
                     isoform X1"
                     /calculated_mol_wt=90880
     Region          <295..384
                     /region_name="rne"
                     /note="ribonuclease E; Reviewed; PRK10811"
                     /db_xref="CDD:236766"
     Region          461..821
                     /region_name="UCH"
                     /note="Ubiquitin carboxyl-terminal hydrolase; pfam00443"
                     /db_xref="CDD:425685"
     CDS             1..831
                     /gene="LOC144113784"
                     /coded_by="XM_077647095.1:138..2633"
                     /db_xref="GeneID:144113784"
ORIGIN      
        1 mgdsrlsyef fnwadiphkd keilhealrn gsdcvelpwq depdcsetlr gappdqpgil
       61 gyaaaldpet algsplqetf diqsaymqpq piyppaqysp ghhhpsiisq emicdygeqh
      121 ppqppmaleg mgkrstgrrg hrnksmhhtp rpdvppppps evlermgdpv rhlpityagg
      181 qsgypfasys phpvfymcvg aaqpyygpaq ghplsfvtav vpsqpphvam aaastmagep
      241 lapshfadpp dlssslvypa rnehhyegte rrrrgsvkpp ragkgrakrg lrghqpkeev
      301 astlttasse eppavvergn lepeavvvpa vtdvapeslq apvnaasptp ctaasvpkke
      361 epavelteaa tsverptpma pvvngsgpam rswadlfkkg sggtegankl hsavpvtsds
      421 arsdcnelrt gpgvpesgdt slgtalhnci vnhtppmiqp rglengknwc yinanlqall
      481 acpafynllh slpvvpgmpg stetplmecf iyflhsyaip lvretgkkde lvigsaydpe
      541 pfrdllmkik pdckkgkqed aeeflsfmln glhdemvssm rsvsnglngq cngeaqapae
      601 hldddddegp wktvshrnrc qvtrsaeysr splmdifgge mrssvtadge tsaslqpfft
      661 lqldiqdgvk tveeallhla aeekvqnyhs sksnkevdal rrttleklpq vlilhlkrfv
      721 ydknggckkl mnaihiphql qldrklvssv ervsskqpcy vlfavlshsg ertdkghyvt
      781 dayhpavrtw lrcdddnvtp iaqgeqlqfe nsslvpyllf yqradtdhkp r
//
LOCUS       XP_077503222             830 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin carboxyl-terminal hydrolase 10-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503222
VERSION     XP_077503222.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647096.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..830
                     /product="ubiquitin carboxyl-terminal hydrolase 10-like
                     isoform X2"
                     /calculated_mol_wt=90882
     Region          <198..380
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     Region          <294..383
                     /region_name="rne"
                     /note="ribonuclease E; Reviewed; PRK10811"
                     /db_xref="CDD:236766"
     Region          460..820
                     /region_name="UCH"
                     /note="Ubiquitin carboxyl-terminal hydrolase; pfam00443"
                     /db_xref="CDD:425685"
     CDS             1..830
                     /gene="LOC144113784"
                     /coded_by="XM_077647096.1:138..2630"
                     /db_xref="GeneID:144113784"
ORIGIN      
        1 mgdsrlsyef fnwadiphkd keilhealrn gsdcvelpwq depdcsetlr gappdqrilg
       61 yaaaldpeta lgsplqetfd iqsaymqpqp iyppaqyspg hhhpsiisqe micdygeqhp
      121 pqppmalegm gkrstgrrgh rnksmhhtpr pdvpppppse vlermgdpvr hlpityaggq
      181 sgypfasysp hpvfymcvga aqpyygpaqg hplsfvtavv psqpphvama aastmagepl
      241 apshfadppd lssslvypar nehhyegter rrrgsvkppr agkgrakrgl rghqpkeeva
      301 stlttassee ppavvergnl epeavvvpav tdvapeslqa pvnaasptpc taasvpkkee
      361 pavelteaat sverptpmap vvngsgpamr swadlfkkgs ggteganklh savpvtsdsa
      421 rsdcnelrtg pgvpesgdts lgtalhnciv nhtppmiqpr glengknwcy inanlqalla
      481 cpafynllhs lpvvpgmpgs tetplmecfi yflhsyaipl vretgkkdel vigsaydpep
      541 frdllmkikp dckkgkqeda eeflsfmlng lhdemvssmr svsnglngqc ngeaqapaeh
      601 ldddddegpw ktvshrnrcq vtrsaeysrs plmdifggem rssvtadget saslqpfftl
      661 qldiqdgvkt veeallhlaa eekvqnyhss ksnkevdalr rttleklpqv lilhlkrfvy
      721 dknggckklm naihiphqlq ldrklvssve rvsskqpcyv lfavlshsge rtdkghyvtd
      781 ayhpavrtwl rcdddnvtpi aqgeqlqfen sslvpyllfy qradtdhkpr
//
LOCUS       XP_077503223             818 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin carboxyl-terminal hydrolase 10-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503223
VERSION     XP_077503223.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647097.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..818
                     /product="ubiquitin carboxyl-terminal hydrolase 10-like
                     isoform X3"
                     /calculated_mol_wt=89283
     Region          <199..378
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          448..808
                     /region_name="UCH"
                     /note="Ubiquitin carboxyl-terminal hydrolase; pfam00443"
                     /db_xref="CDD:425685"
     CDS             1..818
                     /gene="LOC144113784"
                     /coded_by="XM_077647097.1:138..2594"
                     /db_xref="GeneID:144113784"
ORIGIN      
        1 mgdsrlsyef fnwadiphkd keilhealrn gsdcvelpwq depdcsetlr gappdqpgil
       61 gyaaaldpet algsplqetf diqsaymqpq piyppaqysp ghhhpsiisq emicdygeqh
      121 ppqppmaleg mgkrstgrrg hrnksmhhtp rpdvppppps evlermgdpv rhlpityagg
      181 qsgypfasys phpvfymcvg aaqpyygpaq ghplsfvtav vpsqpphvam aaastmagep
      241 lapshfadpp dlssgterrr rgsvkpprag kgrakrglrg hqpkeevast lttasseepp
      301 avvergnlep eavvvpavtd vapeslqapv naasptpcta asvpkkeepa velteaatsv
      361 erptpmapvv ngsgpamrsw adlfkkgsgg teganklhsa vpvtsdsars dcnelrtgpg
      421 vpesgdtslg talhncivnh tppmiqprgl engknwcyin anlqallacp afynllhslp
      481 vvpgmpgste tplmecfiyf lhsyaiplvr etgkkdelvi gsaydpepfr dllmkikpdc
      541 kkgkqedaee flsfmlnglh demvssmrsv snglngqcng eaqapaehld ddddegpwkt
      601 vshrnrcqvt rsaeysrspl mdifggemrs svtadgetsa slqpfftlql diqdgvktve
      661 eallhlaaee kvqnyhssks nkevdalrrt tleklpqvli lhlkrfvydk nggckklmna
      721 ihiphqlqld rklvssverv sskqpcyvlf avlshsgert dkghyvtday hpavrtwlrc
      781 dddnvtpiaq geqlqfenss lvpyllfyqr adtdhkpr
//
LOCUS       XP_077503224             803 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin carboxyl-terminal hydrolase 10-like isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503224
VERSION     XP_077503224.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647098.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..803
                     /product="ubiquitin carboxyl-terminal hydrolase 10-like
                     isoform X4"
                     /calculated_mol_wt=87772
     Region          <207..>413
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          433..793
                     /region_name="UCH"
                     /note="Ubiquitin carboxyl-terminal hydrolase; pfam00443"
                     /db_xref="CDD:425685"
     CDS             1..803
                     /gene="LOC144113784"
                     /coded_by="XM_077647098.1:138..2549"
                     /db_xref="GeneID:144113784"
ORIGIN      
        1 mgdsrlsyef fnwadiphkd keilhealrn gsdcvelpwq depdcsetlr gappdqpgil
       61 gyaaaldpet algsplqetf diqsaymqpq piyppaqysp ghhhpsiisq emicdygeqh
      121 ppqppmaleg mgkrstgrrg hrnksmhhtp rpdvppppps evlermgdpv rhlpityagg
      181 qsgypfasys phpvfymcvg aaqpyygpaq ghplsfvtav vpsqpphvam aaastmagep
      241 lapshfadpp dlssslvypa rnehhyepke evastlttas seeppavver gnlepeavvv
      301 pavtdvapes lqapvnaasp tpctaasvpk keepavelte aatsverptp mapvvngsgp
      361 amrswadlfk kgsggtegan klhsavpvts dsarsdcnel rtgpgvpesg dtslgtalhn
      421 civnhtppmi qprglengkn wcyinanlqa llacpafynl lhslpvvpgm pgstetplme
      481 cfiyflhsya iplvretgkk delvigsayd pepfrdllmk ikpdckkgkq edaeeflsfm
      541 lnglhdemvs smrsvsngln gqcngeaqap aehlddddde gpwktvshrn rcqvtrsaey
      601 srsplmdifg gemrssvtad getsaslqpf ftlqldiqdg vktveeallh laaeekvqny
      661 hssksnkevd alrrttlekl pqvlilhlkr fvydknggck klmnaihiph qlqldrklvs
      721 svervsskqp cyvlfavlsh sgertdkghy vtdayhpavr twlrcdddnv tpiaqgeqlq
      781 fensslvpyl lfyqradtdh kpr
//
LOCUS       XP_077503225             790 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin carboxyl-terminal hydrolase 10-like isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077503225
VERSION     XP_077503225.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647099.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..790
                     /product="ubiquitin carboxyl-terminal hydrolase 10-like
                     isoform X5"
                     /calculated_mol_wt=86175
     Region          <207..>400
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          420..780
                     /region_name="UCH"
                     /note="Ubiquitin carboxyl-terminal hydrolase; pfam00443"
                     /db_xref="CDD:425685"
     CDS             1..790
                     /gene="LOC144113784"
                     /coded_by="XM_077647099.1:138..2510"
                     /db_xref="GeneID:144113784"
ORIGIN      
        1 mgdsrlsyef fnwadiphkd keilhealrn gsdcvelpwq depdcsetlr gappdqpgil
       61 gyaaaldpet algsplqetf diqsaymqpq piyppaqysp ghhhpsiisq emicdygeqh
      121 ppqppmaleg mgkrstgrrg hrnksmhhtp rpdvppppps evlermgdpv rhlpityagg
      181 qsgypfasys phpvfymcvg aaqpyygpaq ghplsfvtav vpsqpphvam aaastmagep
      241 lapshfadpp dlsspkeeva stlttassee ppavvergnl epeavvvpav tdvapeslqa
      301 pvnaasptpc taasvpkkee pavelteaat sverptpmap vvngsgpamr swadlfkkgs
      361 ggteganklh savpvtsdsa rsdcnelrtg pgvpesgdts lgtalhnciv nhtppmiqpr
      421 glengknwcy inanlqalla cpafynllhs lpvvpgmpgs tetplmecfi yflhsyaipl
      481 vretgkkdel vigsaydpep frdllmkikp dckkgkqeda eeflsfmlng lhdemvssmr
      541 svsnglngqc ngeaqapaeh ldddddegpw ktvshrnrcq vtrsaeysrs plmdifggem
      601 rssvtadget saslqpfftl qldiqdgvkt veeallhlaa eekvqnyhss ksnkevdalr
      661 rttleklpqv lilhlkrfvy dknggckklm naihiphqlq ldrklvssve rvsskqpcyv
      721 lfavlshsge rtdkghyvtd ayhpavrtwl rcdddnvtpi aqgeqlqfen sslvpyllfy
      781 qradtdhkpr
//
LOCUS       XP_077503226            1720 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113786 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503226
VERSION     XP_077503226.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647100.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1720
                     /product="uncharacterized protein LOC144113786 isoform X1"
                     /calculated_mol_wt=189013
     Region          186..206
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(186,189,202,206)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(191,193,195,197..198,201..202,205,219,221,225..226,
                     229..230,233,247,249,251,253..254,257..258,261)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          214..234
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          242..262
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          270..286
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          302..322
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(302,305,318,322)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          345..>393
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          347..368
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          347..367
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(347,350,363,367)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(352,354,356,358..359,362..363,366,380,382,386..387,
                     390..391,394,408,410,412,414..415,418..419,423)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Site            order(352,358,365,367,374,381)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          371..810
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          375..395
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          375..393
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          403..424
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          438..459
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          598..618
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(598,601,614,618)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(603,605,607,609..610,613..614,617,631,633,637..638,
                     641..642,646,660,662,664,666..667,670..671,674)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          626..647
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          655..675
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          766..786
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(766,769,782,786)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(771,773,775,777..778,781..782,785,799,801,805..806,
                     809..810,813,827,829,831,833..834,837..838,841)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          794..814
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          820..842
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          822..842
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          834..859
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          850..870
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1093..>1367
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          1099..1119
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1131..1151
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1162..1182
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1282..1302
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1282,1285,1298,1302)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1287,1289,1291,1293..1294,1297..1298,1301,1315,1317,
                     1321..1322,1325..1326,1329,1343,1345,1347,1349..1350,
                     1353..1354,1357)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1294..1318
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1310..1330
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1338..1358
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1350..1375
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1366..1386
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1597..1617
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1597,1600,1613,1617)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          <1619..>1697
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          1631..1651
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1631,1634,1647,1651)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1636,1638,1640,1642..1643,1646..1647,1650,1664,1666,
                     1670..1671,1674..1675,1678,1692,1694,1696,1698..1699,
                     1702..1703)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1644..1668
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1659..1679
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1687..1705
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1720
                     /gene="LOC144113786"
                     /coded_by="XM_077647100.1:335..5497"
                     /db_xref="GeneID:144113786"
ORIGIN      
        1 mgtkllvvfl lwngvsefsf hitmeiqdvr sitengilad asallteasp getavaalpv
       61 vnlsipflgg tqgimigtta dgsgafvvda shlsllgssg vftdgvlqfs vqgnddaeqv
      121 vllqpqeman legsstaplm elspnipavv pplaalpdae aarkspridh kpdeigppvg
      181 kgpfkcsics aefpkwsqvq rherahaedk pfrcsrceas fnqelnlrlh mathpgpgqp
      241 scpecgksfa rlaslkahlm lhqkeenlic aecgdefstq arldrhaqeh kdelsrvkyy
      301 fcrqcpqkfs kqpllkehmk mhykikasls hrtykrnidr ssfshkcttc kkqfqkpsql
      361 lrhirihtge rpfgcnichk tfnqkgalqi hlskhsglrp hrcefcsaaf sqrgnlrahi
      421 qrvhtgpsnd qnaivfrcqe ctctfrrlgs lnahmsrvhs gqmklstsvs rkealetlsk
      481 tlhqqasvvv dntldekdvl drsnadllqq vlvnsglths pstnnseakk lppppeetvp
      541 sdmkensfvp psnpieaaie kvasttesam vmsvadtatg qikmhiyrmv gnvrwhqcmy
      601 ctkefkkpsd lvrhirihtq ekpykcshcf rafavkstlt ahtrathlgv kqfqcptcqh
      661 lfstrgslkv hmrihtgdkp ytcgicnksf sssgrckmhv aahgreadgf rlpsnirgat
      721 gvisedlssf ipiqepifis eaageaaidd gsarrqlttq erpykcsmcl vgfkksshlk
      781 qhirshtgek pfhctecqrs fvsngvlkah vkthtgirey kcavcnatft tngslkrhmc
      841 thtnarpfmc pycqktfkts vnckkhmkih rgelilkrsd pqtqgaevdg ttgavlsdsl
      901 vealvpvnpd evmgqnhngl datipaaevl aqgsslehgd pdgfvaqevl vqefwryfdd
      961 nlqqlqgtlf ggqsdqmdlg qaaqvfsqng ffsqtlpqlt lqgdpldvgt lassfvtssl
     1021 aaptdlaapt elaaptelaa ptelapatse sdelvaelpv snedvspsla qestlqqaaq
     1081 dalrgaevda gdgekpfvct lcdkrfkrsa ylkshmrchs vrmasqknhi cqqcgscfps
     1141 afslkrhsdt hsegllcesf vceacsislp naaqyqqhls lhqssdkekp tddlpsvdpd
     1201 paaavdesqr tsghvvdkas vsatsrkfvt ltdeqsrela rkdprestls vseraliata
     1261 aekdresqew ekppaadypn rcemcpksfr kpsdlarhvr ihtgerpfsc eicqksftvk
     1321 stldthrrth rgernypchi cssffstmgs lkvhmrlhtg srpfkcphcd lrfrtsghrk
     1381 shilthfksg gvrrrsrmlt redkapqvai legapdaegi leeiqlqlap giqitglnps
     1441 tqtvqidatf lqqlqqlqqq gninisitps ttvsngadle qqldagsfli qqdatgsssa
     1501 vldqslplva segkdgavtf tvveggssst vqeeipdvqv aaaelgldel aaaegslmds
     1561 avmdaadfvt pgiiteaage rrkkpvddgd garvhvcdlc sksykrashl kehleshksk
     1621 edkgkkalhq clecpkafsk psqlkrheri htgerpfqct lcsksfnqnn alfvhlikht
     1681 gakpfkcdac gsqftqkcnl mahirrvhkv deaaskepet
//
LOCUS       XP_077503227            1713 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113786 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503227
VERSION     XP_077503227.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647101.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1713
                     /product="uncharacterized protein LOC144113786 isoform X2"
                     /calculated_mol_wt=187969
     Region          186..206
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(186,189,202,206)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(191,193,195,197..198,201..202,205,219,221,225..226,
                     229..230,233,247,249,251,253..254,257..258,261)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          214..234
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          242..262
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          270..286
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          302..322
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(302,305,318,322)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          345..>393
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          347..368
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          347..367
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(347,350,363,367)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(352,354,356,358..359,362..363,366,380,382,386..387,
                     390..391,394,408,410,412,414..415,418..419,423)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Site            order(352,358,365,367,374,381)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          371..810
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          375..395
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          375..393
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          403..424
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          438..459
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          598..618
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(598,601,614,618)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(603,605,607,609..610,613..614,617,631,633,637..638,
                     641..642,646,660,662,664,666..667,670..671,674)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          626..647
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          655..675
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          766..786
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(766,769,782,786)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(771,773,775,777..778,781..782,785,799,801,805..806,
                     809..810,813,827,829,831,833..834,837..838,841)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          794..814
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          820..842
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          822..842
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          834..859
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          850..870
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1086..>1360
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          1092..1112
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1124..1144
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1155..1175
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1275..1295
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1275,1278,1291,1295)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1280,1282,1284,1286..1287,1290..1291,1294,1308,1310,
                     1314..1315,1318..1319,1322,1336,1338,1340,1342..1343,
                     1346..1347,1350)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1287..1311
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1303..1323
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1331..1351
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1343..1368
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1359..1379
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1590..1610
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1590,1593,1606,1610)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          <1612..>1690
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          1624..1644
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1624,1627,1640,1644)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1629,1631,1633,1635..1636,1639..1640,1643,1657,1659,
                     1663..1664,1667..1668,1671,1685,1687,1689,1691..1692,
                     1695..1696)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1637..1661
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1652..1672
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1680..1698
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1713
                     /gene="LOC144113786"
                     /coded_by="XM_077647101.1:1008..6149"
                     /db_xref="GeneID:144113786"
ORIGIN      
        1 mgtkllvvfl lwngvsefsf hitmeiqdvr sitengilad asallteasp getavaalpv
       61 vnlsipflgg tqgimigtta dgsgafvvda shlsllgssg vftdgvlqfs vqgnddaeqv
      121 vllqpqeman legsstaplm elspnipavv pplaalpdae aarkspridh kpdeigppvg
      181 kgpfkcsics aefpkwsqvq rherahaedk pfrcsrceas fnqelnlrlh mathpgpgqp
      241 scpecgksfa rlaslkahlm lhqkeenlic aecgdefstq arldrhaqeh kdelsrvkyy
      301 fcrqcpqkfs kqpllkehmk mhykikasls hrtykrnidr ssfshkcttc kkqfqkpsql
      361 lrhirihtge rpfgcnichk tfnqkgalqi hlskhsglrp hrcefcsaaf sqrgnlrahi
      421 qrvhtgpsnd qnaivfrcqe ctctfrrlgs lnahmsrvhs gqmklstsvs rkealetlsk
      481 tlhqqasvvv dntldekdvl drsnadllqq vlvnsglths pstnnseakk lppppeetvp
      541 sdmkensfvp psnpieaaie kvasttesam vmsvadtatg qikmhiyrmv gnvrwhqcmy
      601 ctkefkkpsd lvrhirihtq ekpykcshcf rafavkstlt ahtrathlgv kqfqcptcqh
      661 lfstrgslkv hmrihtgdkp ytcgicnksf sssgrckmhv aahgreadgf rlpsnirgat
      721 gvisedlssf ipiqepifis eaageaaidd gsarrqlttq erpykcsmcl vgfkksshlk
      781 qhirshtgek pfhctecqrs fvsngvlkah vkthtgirey kcavcnatft tngslkrhmc
      841 thtnarpfmc pycqktfkts vnckkhmkih rgelilkrsd pqtqgaevdg ttgavlsdsl
      901 vealvpvnpd evmgqnhngl datipaaevl aqgsslehgd pdgfvaqevl vqdnlqqlqg
      961 tlfggqsdqm dlgqaaqvfs qngffsqtlp qltlqgdpld vgtlassfvt sslaaptdla
     1021 aptelaapte laaptelapa tsesdelvae lpvsnedvsp slaqestlqq aaqdalrgae
     1081 vdagdgekpf vctlcdkrfk rsaylkshmr chsvrmasqk nhicqqcgsc fpsafslkrh
     1141 sdthsegllc esfvceacsi slpnaaqyqq hlslhqssdk ekptddlpsv dpdpaaavde
     1201 sqrtsghvvd kasvsatsrk fvtltdeqsr elarkdpres tlsvserali ataaekdres
     1261 qewekppaad ypnrcemcpk sfrkpsdlar hvrihtgerp fsceicqksf tvkstldthr
     1321 rthrgernyp chicssffst mgslkvhmrl htgsrpfkcp hcdlrfrtsg hrkshilthf
     1381 ksggvrrrsr mltredkapq vailegapda egileeiqlq lapgiqitgl npstqtvqid
     1441 atflqqlqql qqqgninisi tpsttvsnga dleqqldags fliqqdatgs ssavldqslp
     1501 lvasegkdga vtftvveggs sstvqeeipd vqvaaaelgl delaaaegsl mdsavmdaad
     1561 fvtpgiitea agerrkkpvd dgdgarvhvc dlcsksykra shlkehlesh kskedkgkka
     1621 lhqclecpka fskpsqlkrh erihtgerpf qctlcsksfn qnnalfvhli khtgakpfkc
     1681 dacgsqftqk cnlmahirrv hkvdeaaske pet
//
LOCUS       XP_077503228            1697 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113786 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503228
VERSION     XP_077503228.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647102.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1697
                     /product="uncharacterized protein LOC144113786 isoform X3"
                     /calculated_mol_wt=186392
     Region          163..183
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(163,166,179,183)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(168,170,172,174..175,178..179,182,196,198,202..203,
                     206..207,210,224,226,228,230..231,234..235,238)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          191..211
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          219..239
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          247..263
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          279..299
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(279,282,295,299)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          322..>370
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          324..345
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          324..344
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(324,327,340,344)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(329,331,333,335..336,339..340,343,357,359,363..364,
                     367..368,371,385,387,389,391..392,395..396,400)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Site            order(329,335,342,344,351,358)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          348..787
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          352..372
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          352..370
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          380..401
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          415..436
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          575..595
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(575,578,591,595)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(580,582,584,586..587,590..591,594,608,610,614..615,
                     618..619,623,637,639,641,643..644,647..648,651)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          603..624
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          632..652
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          743..763
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(743,746,759,763)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(748,750,752,754..755,758..759,762,776,778,782..783,
                     786..787,790,804,806,808,810..811,814..815,818)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          771..791
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          797..819
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          799..819
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          811..836
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          827..847
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1070..>1344
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          1076..1096
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1108..1128
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1139..1159
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1259..1279
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1259,1262,1275,1279)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1264,1266,1268,1270..1271,1274..1275,1278,1292,1294,
                     1298..1299,1302..1303,1306,1320,1322,1324,1326..1327,
                     1330..1331,1334)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1271..1295
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1287..1307
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1315..1335
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1327..1352
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1343..1363
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1574..1594
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1574,1577,1590,1594)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          <1596..>1674
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          1608..1628
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1608,1611,1624,1628)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1613,1615,1617,1619..1620,1623..1624,1627,1641,1643,
                     1647..1648,1651..1652,1655,1669,1671,1673,1675..1676,
                     1679..1680)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1621..1645
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1636..1656
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1664..1682
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1697
                     /gene="LOC144113786"
                     /coded_by="XM_077647102.1:363..5456"
                     /db_xref="GeneID:144113786"
ORIGIN      
        1 meiqdvrsit engiladasa llteaspget avaalpvvnl sipflggtqg imigttadgs
       61 gafvvdashl sllgssgvft dgvlqfsvqg nddaeqvvll qpqemanleg sstaplmels
      121 pnipavvppl aalpdaeaar kspridhkpd eigppvgkgp fkcsicsaef pkwsqvqrhe
      181 rahaedkpfr csrceasfnq elnlrlhmat hpgpgqpscp ecgksfarla slkahlmlhq
      241 keenlicaec gdefstqarl drhaqehkde lsrvkyyfcr qcpqkfskqp llkehmkmhy
      301 kikaslshrt ykrnidrssf shkcttckkq fqkpsqllrh irihtgerpf gcnichktfn
      361 qkgalqihls khsglrphrc efcsaafsqr gnlrahiqrv htgpsndqna ivfrcqectc
      421 tfrrlgslna hmsrvhsgqm klstsvsrke aletlsktlh qqasvvvdnt ldekdvldrs
      481 nadllqqvlv nsglthspst nnseakklpp ppeetvpsdm kensfvppsn pieaaiekva
      541 sttesamvms vadtatgqik mhiyrmvgnv rwhqcmyctk efkkpsdlvr hirihtqekp
      601 ykcshcfraf avkstltaht rathlgvkqf qcptcqhlfs trgslkvhmr ihtgdkpytc
      661 gicnksfsss grckmhvaah greadgfrlp snirgatgvi sedlssfipi qepifiseaa
      721 geaaiddgsa rrqlttqerp ykcsmclvgf kksshlkqhi rshtgekpfh ctecqrsfvs
      781 ngvlkahvkt htgireykca vcnatfttng slkrhmctht narpfmcpyc qktfktsvnc
      841 kkhmkihrge lilkrsdpqt qgaevdgttg avlsdslvea lvpvnpdevm gqnhngldat
      901 ipaaevlaqg sslehgdpdg fvaqevlvqe fwryfddnlq qlqgtlfggq sdqmdlgqaa
      961 qvfsqngffs qtlpqltlqg dpldvgtlas sfvtsslaap tdlaaptela aptelaapte
     1021 lapatsesde lvaelpvsne dvspslaqes tlqqaaqdal rgaevdagdg ekpfvctlcd
     1081 krfkrsaylk shmrchsvrm asqknhicqq cgscfpsafs lkrhsdthse gllcesfvce
     1141 acsislpnaa qyqqhlslhq ssdkekptdd lpsvdpdpaa avdesqrtsg hvvdkasvsa
     1201 tsrkfvtltd eqsrelarkd prestlsvse raliataaek dresqewekp paadypnrce
     1261 mcpksfrkps dlarhvriht gerpfsceic qksftvkstl dthrrthrge rnypchicss
     1321 ffstmgslkv hmrlhtgsrp fkcphcdlrf rtsghrkshi lthfksggvr rrsrmltred
     1381 kapqvaileg apdaegilee iqlqlapgiq itglnpstqt vqidatflqq lqqlqqqgni
     1441 nisitpsttv sngadleqql dagsfliqqd atgsssavld qslplvaseg kdgavtftvv
     1501 eggssstvqe eipdvqvaaa elgldelaaa egslmdsavm daadfvtpgi iteaagerrk
     1561 kpvddgdgar vhvcdlcsks ykrashlkeh leshkskedk gkkalhqcle cpkafskpsq
     1621 lkrherihtg erpfqctlcs ksfnqnnalf vhlikhtgak pfkcdacgsq ftqkcnlmah
     1681 irrvhkvdea askepet
//
LOCUS       XP_077503229            1697 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113786 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503229
VERSION     XP_077503229.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647103.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1697
                     /product="uncharacterized protein LOC144113786 isoform X3"
                     /calculated_mol_wt=186392
     Region          163..183
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(163,166,179,183)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(168,170,172,174..175,178..179,182,196,198,202..203,
                     206..207,210,224,226,228,230..231,234..235,238)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          191..211
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          219..239
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          247..263
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          279..299
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(279,282,295,299)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          322..>370
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          324..345
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          324..344
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(324,327,340,344)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(329,331,333,335..336,339..340,343,357,359,363..364,
                     367..368,371,385,387,389,391..392,395..396,400)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Site            order(329,335,342,344,351,358)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          348..787
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          352..372
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          352..370
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          380..401
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          415..436
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          575..595
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(575,578,591,595)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(580,582,584,586..587,590..591,594,608,610,614..615,
                     618..619,623,637,639,641,643..644,647..648,651)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          603..624
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          632..652
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          743..763
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(743,746,759,763)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(748,750,752,754..755,758..759,762,776,778,782..783,
                     786..787,790,804,806,808,810..811,814..815,818)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          771..791
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          797..819
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          799..819
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          811..836
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          827..847
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1070..>1344
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          1076..1096
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1108..1128
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1139..1159
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1259..1279
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1259,1262,1275,1279)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1264,1266,1268,1270..1271,1274..1275,1278,1292,1294,
                     1298..1299,1302..1303,1306,1320,1322,1324,1326..1327,
                     1330..1331,1334)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1271..1295
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1287..1307
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1315..1335
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1327..1352
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1343..1363
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1574..1594
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1574,1577,1590,1594)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          <1596..>1674
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          1608..1628
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1608,1611,1624,1628)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1613,1615,1617,1619..1620,1623..1624,1627,1641,1643,
                     1647..1648,1651..1652,1655,1669,1671,1673,1675..1676,
                     1679..1680)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1621..1645
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1636..1656
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1664..1682
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1697
                     /gene="LOC144113786"
                     /coded_by="XM_077647103.1:425..5518"
                     /db_xref="GeneID:144113786"
ORIGIN      
        1 meiqdvrsit engiladasa llteaspget avaalpvvnl sipflggtqg imigttadgs
       61 gafvvdashl sllgssgvft dgvlqfsvqg nddaeqvvll qpqemanleg sstaplmels
      121 pnipavvppl aalpdaeaar kspridhkpd eigppvgkgp fkcsicsaef pkwsqvqrhe
      181 rahaedkpfr csrceasfnq elnlrlhmat hpgpgqpscp ecgksfarla slkahlmlhq
      241 keenlicaec gdefstqarl drhaqehkde lsrvkyyfcr qcpqkfskqp llkehmkmhy
      301 kikaslshrt ykrnidrssf shkcttckkq fqkpsqllrh irihtgerpf gcnichktfn
      361 qkgalqihls khsglrphrc efcsaafsqr gnlrahiqrv htgpsndqna ivfrcqectc
      421 tfrrlgslna hmsrvhsgqm klstsvsrke aletlsktlh qqasvvvdnt ldekdvldrs
      481 nadllqqvlv nsglthspst nnseakklpp ppeetvpsdm kensfvppsn pieaaiekva
      541 sttesamvms vadtatgqik mhiyrmvgnv rwhqcmyctk efkkpsdlvr hirihtqekp
      601 ykcshcfraf avkstltaht rathlgvkqf qcptcqhlfs trgslkvhmr ihtgdkpytc
      661 gicnksfsss grckmhvaah greadgfrlp snirgatgvi sedlssfipi qepifiseaa
      721 geaaiddgsa rrqlttqerp ykcsmclvgf kksshlkqhi rshtgekpfh ctecqrsfvs
      781 ngvlkahvkt htgireykca vcnatfttng slkrhmctht narpfmcpyc qktfktsvnc
      841 kkhmkihrge lilkrsdpqt qgaevdgttg avlsdslvea lvpvnpdevm gqnhngldat
      901 ipaaevlaqg sslehgdpdg fvaqevlvqe fwryfddnlq qlqgtlfggq sdqmdlgqaa
      961 qvfsqngffs qtlpqltlqg dpldvgtlas sfvtsslaap tdlaaptela aptelaapte
     1021 lapatsesde lvaelpvsne dvspslaqes tlqqaaqdal rgaevdagdg ekpfvctlcd
     1081 krfkrsaylk shmrchsvrm asqknhicqq cgscfpsafs lkrhsdthse gllcesfvce
     1141 acsislpnaa qyqqhlslhq ssdkekptdd lpsvdpdpaa avdesqrtsg hvvdkasvsa
     1201 tsrkfvtltd eqsrelarkd prestlsvse raliataaek dresqewekp paadypnrce
     1261 mcpksfrkps dlarhvriht gerpfsceic qksftvkstl dthrrthrge rnypchicss
     1321 ffstmgslkv hmrlhtgsrp fkcphcdlrf rtsghrkshi lthfksggvr rrsrmltred
     1381 kapqvaileg apdaegilee iqlqlapgiq itglnpstqt vqidatflqq lqqlqqqgni
     1441 nisitpsttv sngadleqql dagsfliqqd atgsssavld qslplvaseg kdgavtftvv
     1501 eggssstvqe eipdvqvaaa elgldelaaa egslmdsavm daadfvtpgi iteaagerrk
     1561 kpvddgdgar vhvcdlcsks ykrashlkeh leshkskedk gkkalhqcle cpkafskpsq
     1621 lkrherihtg erpfqctlcs ksfnqnnalf vhlikhtgak pfkcdacgsq ftqkcnlmah
     1681 irrvhkvdea askepet
//
LOCUS       XP_077503230            1697 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113786 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503230
VERSION     XP_077503230.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647104.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1697
                     /product="uncharacterized protein LOC144113786 isoform X3"
                     /calculated_mol_wt=186392
     Region          163..183
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(163,166,179,183)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(168,170,172,174..175,178..179,182,196,198,202..203,
                     206..207,210,224,226,228,230..231,234..235,238)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          191..211
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          219..239
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          247..263
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          279..299
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(279,282,295,299)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          322..>370
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          324..345
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          324..344
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(324,327,340,344)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(329,331,333,335..336,339..340,343,357,359,363..364,
                     367..368,371,385,387,389,391..392,395..396,400)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Site            order(329,335,342,344,351,358)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          348..787
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          352..372
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          352..370
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          380..401
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          415..436
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          575..595
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(575,578,591,595)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(580,582,584,586..587,590..591,594,608,610,614..615,
                     618..619,623,637,639,641,643..644,647..648,651)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          603..624
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          632..652
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          743..763
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(743,746,759,763)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(748,750,752,754..755,758..759,762,776,778,782..783,
                     786..787,790,804,806,808,810..811,814..815,818)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          771..791
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          797..819
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          799..819
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          811..836
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          827..847
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1070..>1344
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          1076..1096
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1108..1128
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1139..1159
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1259..1279
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1259,1262,1275,1279)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1264,1266,1268,1270..1271,1274..1275,1278,1292,1294,
                     1298..1299,1302..1303,1306,1320,1322,1324,1326..1327,
                     1330..1331,1334)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1271..1295
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1287..1307
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1315..1335
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1327..1352
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1343..1363
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1574..1594
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1574,1577,1590,1594)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          <1596..>1674
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          1608..1628
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1608,1611,1624,1628)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1613,1615,1617,1619..1620,1623..1624,1627,1641,1643,
                     1647..1648,1651..1652,1655,1669,1671,1673,1675..1676,
                     1679..1680)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1621..1645
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1636..1656
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1664..1682
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1697
                     /gene="LOC144113786"
                     /coded_by="XM_077647104.1:1011..6104"
                     /db_xref="GeneID:144113786"
ORIGIN      
        1 meiqdvrsit engiladasa llteaspget avaalpvvnl sipflggtqg imigttadgs
       61 gafvvdashl sllgssgvft dgvlqfsvqg nddaeqvvll qpqemanleg sstaplmels
      121 pnipavvppl aalpdaeaar kspridhkpd eigppvgkgp fkcsicsaef pkwsqvqrhe
      181 rahaedkpfr csrceasfnq elnlrlhmat hpgpgqpscp ecgksfarla slkahlmlhq
      241 keenlicaec gdefstqarl drhaqehkde lsrvkyyfcr qcpqkfskqp llkehmkmhy
      301 kikaslshrt ykrnidrssf shkcttckkq fqkpsqllrh irihtgerpf gcnichktfn
      361 qkgalqihls khsglrphrc efcsaafsqr gnlrahiqrv htgpsndqna ivfrcqectc
      421 tfrrlgslna hmsrvhsgqm klstsvsrke aletlsktlh qqasvvvdnt ldekdvldrs
      481 nadllqqvlv nsglthspst nnseakklpp ppeetvpsdm kensfvppsn pieaaiekva
      541 sttesamvms vadtatgqik mhiyrmvgnv rwhqcmyctk efkkpsdlvr hirihtqekp
      601 ykcshcfraf avkstltaht rathlgvkqf qcptcqhlfs trgslkvhmr ihtgdkpytc
      661 gicnksfsss grckmhvaah greadgfrlp snirgatgvi sedlssfipi qepifiseaa
      721 geaaiddgsa rrqlttqerp ykcsmclvgf kksshlkqhi rshtgekpfh ctecqrsfvs
      781 ngvlkahvkt htgireykca vcnatfttng slkrhmctht narpfmcpyc qktfktsvnc
      841 kkhmkihrge lilkrsdpqt qgaevdgttg avlsdslvea lvpvnpdevm gqnhngldat
      901 ipaaevlaqg sslehgdpdg fvaqevlvqe fwryfddnlq qlqgtlfggq sdqmdlgqaa
      961 qvfsqngffs qtlpqltlqg dpldvgtlas sfvtsslaap tdlaaptela aptelaapte
     1021 lapatsesde lvaelpvsne dvspslaqes tlqqaaqdal rgaevdagdg ekpfvctlcd
     1081 krfkrsaylk shmrchsvrm asqknhicqq cgscfpsafs lkrhsdthse gllcesfvce
     1141 acsislpnaa qyqqhlslhq ssdkekptdd lpsvdpdpaa avdesqrtsg hvvdkasvsa
     1201 tsrkfvtltd eqsrelarkd prestlsvse raliataaek dresqewekp paadypnrce
     1261 mcpksfrkps dlarhvriht gerpfsceic qksftvkstl dthrrthrge rnypchicss
     1321 ffstmgslkv hmrlhtgsrp fkcphcdlrf rtsghrkshi lthfksggvr rrsrmltred
     1381 kapqvaileg apdaegilee iqlqlapgiq itglnpstqt vqidatflqq lqqlqqqgni
     1441 nisitpsttv sngadleqql dagsfliqqd atgsssavld qslplvaseg kdgavtftvv
     1501 eggssstvqe eipdvqvaaa elgldelaaa egslmdsavm daadfvtpgi iteaagerrk
     1561 kpvddgdgar vhvcdlcsks ykrashlkeh leshkskedk gkkalhqcle cpkafskpsq
     1621 lkrherihtg erpfqctlcs ksfnqnnalf vhlikhtgak pfkcdacgsq ftqkcnlmah
     1681 irrvhkvdea askepet
//
LOCUS       XP_077503231            1697 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113786 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503231
VERSION     XP_077503231.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647105.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1697
                     /product="uncharacterized protein LOC144113786 isoform X3"
                     /calculated_mol_wt=186392
     Region          163..183
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(163,166,179,183)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(168,170,172,174..175,178..179,182,196,198,202..203,
                     206..207,210,224,226,228,230..231,234..235,238)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          191..211
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          219..239
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          247..263
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          279..299
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(279,282,295,299)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          322..>370
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          324..345
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          324..344
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(324,327,340,344)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(329,331,333,335..336,339..340,343,357,359,363..364,
                     367..368,371,385,387,389,391..392,395..396,400)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Site            order(329,335,342,344,351,358)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          348..787
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          352..372
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          352..370
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          380..401
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          415..436
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          575..595
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(575,578,591,595)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(580,582,584,586..587,590..591,594,608,610,614..615,
                     618..619,623,637,639,641,643..644,647..648,651)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          603..624
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          632..652
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          743..763
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(743,746,759,763)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(748,750,752,754..755,758..759,762,776,778,782..783,
                     786..787,790,804,806,808,810..811,814..815,818)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          771..791
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          797..819
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          799..819
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          811..836
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          827..847
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1070..>1344
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          1076..1096
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1108..1128
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1139..1159
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1259..1279
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1259,1262,1275,1279)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1264,1266,1268,1270..1271,1274..1275,1278,1292,1294,
                     1298..1299,1302..1303,1306,1320,1322,1324,1326..1327,
                     1330..1331,1334)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1271..1295
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1287..1307
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1315..1335
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1327..1352
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1343..1363
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1574..1594
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1574,1577,1590,1594)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          <1596..>1674
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          1608..1628
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1608,1611,1624,1628)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1613,1615,1617,1619..1620,1623..1624,1627,1641,1643,
                     1647..1648,1651..1652,1655,1669,1671,1673,1675..1676,
                     1679..1680)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1621..1645
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1636..1656
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1664..1682
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1697
                     /gene="LOC144113786"
                     /coded_by="XM_077647105.1:275..5368"
                     /db_xref="GeneID:144113786"
ORIGIN      
        1 meiqdvrsit engiladasa llteaspget avaalpvvnl sipflggtqg imigttadgs
       61 gafvvdashl sllgssgvft dgvlqfsvqg nddaeqvvll qpqemanleg sstaplmels
      121 pnipavvppl aalpdaeaar kspridhkpd eigppvgkgp fkcsicsaef pkwsqvqrhe
      181 rahaedkpfr csrceasfnq elnlrlhmat hpgpgqpscp ecgksfarla slkahlmlhq
      241 keenlicaec gdefstqarl drhaqehkde lsrvkyyfcr qcpqkfskqp llkehmkmhy
      301 kikaslshrt ykrnidrssf shkcttckkq fqkpsqllrh irihtgerpf gcnichktfn
      361 qkgalqihls khsglrphrc efcsaafsqr gnlrahiqrv htgpsndqna ivfrcqectc
      421 tfrrlgslna hmsrvhsgqm klstsvsrke aletlsktlh qqasvvvdnt ldekdvldrs
      481 nadllqqvlv nsglthspst nnseakklpp ppeetvpsdm kensfvppsn pieaaiekva
      541 sttesamvms vadtatgqik mhiyrmvgnv rwhqcmyctk efkkpsdlvr hirihtqekp
      601 ykcshcfraf avkstltaht rathlgvkqf qcptcqhlfs trgslkvhmr ihtgdkpytc
      661 gicnksfsss grckmhvaah greadgfrlp snirgatgvi sedlssfipi qepifiseaa
      721 geaaiddgsa rrqlttqerp ykcsmclvgf kksshlkqhi rshtgekpfh ctecqrsfvs
      781 ngvlkahvkt htgireykca vcnatfttng slkrhmctht narpfmcpyc qktfktsvnc
      841 kkhmkihrge lilkrsdpqt qgaevdgttg avlsdslvea lvpvnpdevm gqnhngldat
      901 ipaaevlaqg sslehgdpdg fvaqevlvqe fwryfddnlq qlqgtlfggq sdqmdlgqaa
      961 qvfsqngffs qtlpqltlqg dpldvgtlas sfvtsslaap tdlaaptela aptelaapte
     1021 lapatsesde lvaelpvsne dvspslaqes tlqqaaqdal rgaevdagdg ekpfvctlcd
     1081 krfkrsaylk shmrchsvrm asqknhicqq cgscfpsafs lkrhsdthse gllcesfvce
     1141 acsislpnaa qyqqhlslhq ssdkekptdd lpsvdpdpaa avdesqrtsg hvvdkasvsa
     1201 tsrkfvtltd eqsrelarkd prestlsvse raliataaek dresqewekp paadypnrce
     1261 mcpksfrkps dlarhvriht gerpfsceic qksftvkstl dthrrthrge rnypchicss
     1321 ffstmgslkv hmrlhtgsrp fkcphcdlrf rtsghrkshi lthfksggvr rrsrmltred
     1381 kapqvaileg apdaegilee iqlqlapgiq itglnpstqt vqidatflqq lqqlqqqgni
     1441 nisitpsttv sngadleqql dagsfliqqd atgsssavld qslplvaseg kdgavtftvv
     1501 eggssstvqe eipdvqvaaa elgldelaaa egslmdsavm daadfvtpgi iteaagerrk
     1561 kpvddgdgar vhvcdlcsks ykrashlkeh leshkskedk gkkalhqcle cpkafskpsq
     1621 lkrherihtg erpfqctlcs ksfnqnnalf vhlikhtgak pfkcdacgsq ftqkcnlmah
     1681 irrvhkvdea askepet
//
LOCUS       XP_077503232            1690 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113786 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503232
VERSION     XP_077503232.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647106.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1690
                     /product="uncharacterized protein LOC144113786 isoform X4"
                     /calculated_mol_wt=185348
     Region          163..183
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(163,166,179,183)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(168,170,172,174..175,178..179,182,196,198,202..203,
                     206..207,210,224,226,228,230..231,234..235,238)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          191..211
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          219..239
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          247..263
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          279..299
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(279,282,295,299)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          322..>370
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          324..345
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          324..344
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(324,327,340,344)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(329,331,333,335..336,339..340,343,357,359,363..364,
                     367..368,371,385,387,389,391..392,395..396,400)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Site            order(329,335,342,344,351,358)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          348..787
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          352..372
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          352..370
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          380..401
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          415..436
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          575..595
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(575,578,591,595)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(580,582,584,586..587,590..591,594,608,610,614..615,
                     618..619,623,637,639,641,643..644,647..648,651)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          603..624
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          632..652
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          743..763
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(743,746,759,763)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(748,750,752,754..755,758..759,762,776,778,782..783,
                     786..787,790,804,806,808,810..811,814..815,818)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          771..791
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          797..819
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          799..819
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          811..836
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          827..847
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1063..>1337
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          1069..1089
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1101..1121
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1132..1152
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1252..1272
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1252,1255,1268,1272)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1257,1259,1261,1263..1264,1267..1268,1271,1285,1287,
                     1291..1292,1295..1296,1299,1313,1315,1317,1319..1320,
                     1323..1324,1327)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1264..1288
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1280..1300
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1308..1328
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1320..1345
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1336..1356
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1567..1587
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1567,1570,1583,1587)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          <1589..>1667
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          1601..1621
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1601,1604,1617,1621)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1606,1608,1610,1612..1613,1616..1617,1620,1634,1636,
                     1640..1641,1644..1645,1648,1662,1664,1666,1668..1669,
                     1672..1673)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1614..1638
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1629..1649
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1657..1675
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1690
                     /gene="LOC144113786"
                     /coded_by="XM_077647106.1:362..5434"
                     /db_xref="GeneID:144113786"
ORIGIN      
        1 meiqdvrsit engiladasa llteaspget avaalpvvnl sipflggtqg imigttadgs
       61 gafvvdashl sllgssgvft dgvlqfsvqg nddaeqvvll qpqemanleg sstaplmels
      121 pnipavvppl aalpdaeaar kspridhkpd eigppvgkgp fkcsicsaef pkwsqvqrhe
      181 rahaedkpfr csrceasfnq elnlrlhmat hpgpgqpscp ecgksfarla slkahlmlhq
      241 keenlicaec gdefstqarl drhaqehkde lsrvkyyfcr qcpqkfskqp llkehmkmhy
      301 kikaslshrt ykrnidrssf shkcttckkq fqkpsqllrh irihtgerpf gcnichktfn
      361 qkgalqihls khsglrphrc efcsaafsqr gnlrahiqrv htgpsndqna ivfrcqectc
      421 tfrrlgslna hmsrvhsgqm klstsvsrke aletlsktlh qqasvvvdnt ldekdvldrs
      481 nadllqqvlv nsglthspst nnseakklpp ppeetvpsdm kensfvppsn pieaaiekva
      541 sttesamvms vadtatgqik mhiyrmvgnv rwhqcmyctk efkkpsdlvr hirihtqekp
      601 ykcshcfraf avkstltaht rathlgvkqf qcptcqhlfs trgslkvhmr ihtgdkpytc
      661 gicnksfsss grckmhvaah greadgfrlp snirgatgvi sedlssfipi qepifiseaa
      721 geaaiddgsa rrqlttqerp ykcsmclvgf kksshlkqhi rshtgekpfh ctecqrsfvs
      781 ngvlkahvkt htgireykca vcnatfttng slkrhmctht narpfmcpyc qktfktsvnc
      841 kkhmkihrge lilkrsdpqt qgaevdgttg avlsdslvea lvpvnpdevm gqnhngldat
      901 ipaaevlaqg sslehgdpdg fvaqevlvqd nlqqlqgtlf ggqsdqmdlg qaaqvfsqng
      961 ffsqtlpqlt lqgdpldvgt lassfvtssl aaptdlaapt elaaptelaa ptelapatse
     1021 sdelvaelpv snedvspsla qestlqqaaq dalrgaevda gdgekpfvct lcdkrfkrsa
     1081 ylkshmrchs vrmasqknhi cqqcgscfps afslkrhsdt hsegllcesf vceacsislp
     1141 naaqyqqhls lhqssdkekp tddlpsvdpd paaavdesqr tsghvvdkas vsatsrkfvt
     1201 ltdeqsrela rkdprestls vseraliata aekdresqew ekppaadypn rcemcpksfr
     1261 kpsdlarhvr ihtgerpfsc eicqksftvk stldthrrth rgernypchi cssffstmgs
     1321 lkvhmrlhtg srpfkcphcd lrfrtsghrk shilthfksg gvrrrsrmlt redkapqvai
     1381 legapdaegi leeiqlqlap giqitglnps tqtvqidatf lqqlqqlqqq gninisitps
     1441 ttvsngadle qqldagsfli qqdatgsssa vldqslplva segkdgavtf tvveggssst
     1501 vqeeipdvqv aaaelgldel aaaegslmds avmdaadfvt pgiiteaage rrkkpvddgd
     1561 garvhvcdlc sksykrashl kehleshksk edkgkkalhq clecpkafsk psqlkrheri
     1621 htgerpfqct lcsksfnqnn alfvhlikht gakpfkcdac gsqftqkcnl mahirrvhkv
     1681 deaaskepet
//
LOCUS       XP_077503233            1690 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113786 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503233
VERSION     XP_077503233.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647107.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1690
                     /product="uncharacterized protein LOC144113786 isoform X4"
                     /calculated_mol_wt=185348
     Region          163..183
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(163,166,179,183)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(168,170,172,174..175,178..179,182,196,198,202..203,
                     206..207,210,224,226,228,230..231,234..235,238)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          191..211
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          219..239
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          247..263
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          279..299
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(279,282,295,299)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          322..>370
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          324..345
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          324..344
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(324,327,340,344)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(329,331,333,335..336,339..340,343,357,359,363..364,
                     367..368,371,385,387,389,391..392,395..396,400)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Site            order(329,335,342,344,351,358)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          348..787
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          352..372
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          352..370
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          380..401
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          415..436
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          575..595
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(575,578,591,595)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(580,582,584,586..587,590..591,594,608,610,614..615,
                     618..619,623,637,639,641,643..644,647..648,651)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          603..624
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          632..652
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          743..763
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(743,746,759,763)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(748,750,752,754..755,758..759,762,776,778,782..783,
                     786..787,790,804,806,808,810..811,814..815,818)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          771..791
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          797..819
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          799..819
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          811..836
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          827..847
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1063..>1337
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          1069..1089
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1101..1121
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1132..1152
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1252..1272
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1252,1255,1268,1272)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1257,1259,1261,1263..1264,1267..1268,1271,1285,1287,
                     1291..1292,1295..1296,1299,1313,1315,1317,1319..1320,
                     1323..1324,1327)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1264..1288
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1280..1300
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1308..1328
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1320..1345
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1336..1356
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1567..1587
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1567,1570,1583,1587)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          <1589..>1667
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          1601..1621
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1601,1604,1617,1621)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1606,1608,1610,1612..1613,1616..1617,1620,1634,1636,
                     1640..1641,1644..1645,1648,1662,1664,1666,1668..1669,
                     1672..1673)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1614..1638
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1629..1649
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1657..1675
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1690
                     /gene="LOC144113786"
                     /coded_by="XM_077647107.1:1098..6170"
                     /db_xref="GeneID:144113786"
ORIGIN      
        1 meiqdvrsit engiladasa llteaspget avaalpvvnl sipflggtqg imigttadgs
       61 gafvvdashl sllgssgvft dgvlqfsvqg nddaeqvvll qpqemanleg sstaplmels
      121 pnipavvppl aalpdaeaar kspridhkpd eigppvgkgp fkcsicsaef pkwsqvqrhe
      181 rahaedkpfr csrceasfnq elnlrlhmat hpgpgqpscp ecgksfarla slkahlmlhq
      241 keenlicaec gdefstqarl drhaqehkde lsrvkyyfcr qcpqkfskqp llkehmkmhy
      301 kikaslshrt ykrnidrssf shkcttckkq fqkpsqllrh irihtgerpf gcnichktfn
      361 qkgalqihls khsglrphrc efcsaafsqr gnlrahiqrv htgpsndqna ivfrcqectc
      421 tfrrlgslna hmsrvhsgqm klstsvsrke aletlsktlh qqasvvvdnt ldekdvldrs
      481 nadllqqvlv nsglthspst nnseakklpp ppeetvpsdm kensfvppsn pieaaiekva
      541 sttesamvms vadtatgqik mhiyrmvgnv rwhqcmyctk efkkpsdlvr hirihtqekp
      601 ykcshcfraf avkstltaht rathlgvkqf qcptcqhlfs trgslkvhmr ihtgdkpytc
      661 gicnksfsss grckmhvaah greadgfrlp snirgatgvi sedlssfipi qepifiseaa
      721 geaaiddgsa rrqlttqerp ykcsmclvgf kksshlkqhi rshtgekpfh ctecqrsfvs
      781 ngvlkahvkt htgireykca vcnatfttng slkrhmctht narpfmcpyc qktfktsvnc
      841 kkhmkihrge lilkrsdpqt qgaevdgttg avlsdslvea lvpvnpdevm gqnhngldat
      901 ipaaevlaqg sslehgdpdg fvaqevlvqd nlqqlqgtlf ggqsdqmdlg qaaqvfsqng
      961 ffsqtlpqlt lqgdpldvgt lassfvtssl aaptdlaapt elaaptelaa ptelapatse
     1021 sdelvaelpv snedvspsla qestlqqaaq dalrgaevda gdgekpfvct lcdkrfkrsa
     1081 ylkshmrchs vrmasqknhi cqqcgscfps afslkrhsdt hsegllcesf vceacsislp
     1141 naaqyqqhls lhqssdkekp tddlpsvdpd paaavdesqr tsghvvdkas vsatsrkfvt
     1201 ltdeqsrela rkdprestls vseraliata aekdresqew ekppaadypn rcemcpksfr
     1261 kpsdlarhvr ihtgerpfsc eicqksftvk stldthrrth rgernypchi cssffstmgs
     1321 lkvhmrlhtg srpfkcphcd lrfrtsghrk shilthfksg gvrrrsrmlt redkapqvai
     1381 legapdaegi leeiqlqlap giqitglnps tqtvqidatf lqqlqqlqqq gninisitps
     1441 ttvsngadle qqldagsfli qqdatgsssa vldqslplva segkdgavtf tvveggssst
     1501 vqeeipdvqv aaaelgldel aaaegslmds avmdaadfvt pgiiteaage rrkkpvddgd
     1561 garvhvcdlc sksykrashl kehleshksk edkgkkalhq clecpkafsk psqlkrheri
     1621 htgerpfqct lcsksfnqnn alfvhlikht gakpfkcdac gsqftqkcnl mahirrvhkv
     1681 deaaskepet
//
LOCUS       XP_077503234            1690 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113786 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503234
VERSION     XP_077503234.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647108.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1690
                     /product="uncharacterized protein LOC144113786 isoform X4"
                     /calculated_mol_wt=185348
     Region          163..183
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(163,166,179,183)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(168,170,172,174..175,178..179,182,196,198,202..203,
                     206..207,210,224,226,228,230..231,234..235,238)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          191..211
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          219..239
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          247..263
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          279..299
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(279,282,295,299)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          322..>370
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          324..345
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          324..344
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(324,327,340,344)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(329,331,333,335..336,339..340,343,357,359,363..364,
                     367..368,371,385,387,389,391..392,395..396,400)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Site            order(329,335,342,344,351,358)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          348..787
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          352..372
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          352..370
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          380..401
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          415..436
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          575..595
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(575,578,591,595)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(580,582,584,586..587,590..591,594,608,610,614..615,
                     618..619,623,637,639,641,643..644,647..648,651)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          603..624
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          632..652
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          743..763
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(743,746,759,763)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(748,750,752,754..755,758..759,762,776,778,782..783,
                     786..787,790,804,806,808,810..811,814..815,818)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          771..791
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          797..819
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          799..819
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          811..836
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          827..847
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1063..>1337
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          1069..1089
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1101..1121
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1132..1152
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1252..1272
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1252,1255,1268,1272)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1257,1259,1261,1263..1264,1267..1268,1271,1285,1287,
                     1291..1292,1295..1296,1299,1313,1315,1317,1319..1320,
                     1323..1324,1327)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1264..1288
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1280..1300
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1308..1328
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1320..1345
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1336..1356
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1567..1587
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1567,1570,1583,1587)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          <1589..>1667
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          1601..1621
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1601,1604,1617,1621)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1606,1608,1610,1612..1613,1616..1617,1620,1634,1636,
                     1640..1641,1644..1645,1648,1662,1664,1666,1668..1669,
                     1672..1673)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1614..1638
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1629..1649
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1657..1675
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1690
                     /gene="LOC144113786"
                     /coded_by="XM_077647108.1:425..5497"
                     /db_xref="GeneID:144113786"
ORIGIN      
        1 meiqdvrsit engiladasa llteaspget avaalpvvnl sipflggtqg imigttadgs
       61 gafvvdashl sllgssgvft dgvlqfsvqg nddaeqvvll qpqemanleg sstaplmels
      121 pnipavvppl aalpdaeaar kspridhkpd eigppvgkgp fkcsicsaef pkwsqvqrhe
      181 rahaedkpfr csrceasfnq elnlrlhmat hpgpgqpscp ecgksfarla slkahlmlhq
      241 keenlicaec gdefstqarl drhaqehkde lsrvkyyfcr qcpqkfskqp llkehmkmhy
      301 kikaslshrt ykrnidrssf shkcttckkq fqkpsqllrh irihtgerpf gcnichktfn
      361 qkgalqihls khsglrphrc efcsaafsqr gnlrahiqrv htgpsndqna ivfrcqectc
      421 tfrrlgslna hmsrvhsgqm klstsvsrke aletlsktlh qqasvvvdnt ldekdvldrs
      481 nadllqqvlv nsglthspst nnseakklpp ppeetvpsdm kensfvppsn pieaaiekva
      541 sttesamvms vadtatgqik mhiyrmvgnv rwhqcmyctk efkkpsdlvr hirihtqekp
      601 ykcshcfraf avkstltaht rathlgvkqf qcptcqhlfs trgslkvhmr ihtgdkpytc
      661 gicnksfsss grckmhvaah greadgfrlp snirgatgvi sedlssfipi qepifiseaa
      721 geaaiddgsa rrqlttqerp ykcsmclvgf kksshlkqhi rshtgekpfh ctecqrsfvs
      781 ngvlkahvkt htgireykca vcnatfttng slkrhmctht narpfmcpyc qktfktsvnc
      841 kkhmkihrge lilkrsdpqt qgaevdgttg avlsdslvea lvpvnpdevm gqnhngldat
      901 ipaaevlaqg sslehgdpdg fvaqevlvqd nlqqlqgtlf ggqsdqmdlg qaaqvfsqng
      961 ffsqtlpqlt lqgdpldvgt lassfvtssl aaptdlaapt elaaptelaa ptelapatse
     1021 sdelvaelpv snedvspsla qestlqqaaq dalrgaevda gdgekpfvct lcdkrfkrsa
     1081 ylkshmrchs vrmasqknhi cqqcgscfps afslkrhsdt hsegllcesf vceacsislp
     1141 naaqyqqhls lhqssdkekp tddlpsvdpd paaavdesqr tsghvvdkas vsatsrkfvt
     1201 ltdeqsrela rkdprestls vseraliata aekdresqew ekppaadypn rcemcpksfr
     1261 kpsdlarhvr ihtgerpfsc eicqksftvk stldthrrth rgernypchi cssffstmgs
     1321 lkvhmrlhtg srpfkcphcd lrfrtsghrk shilthfksg gvrrrsrmlt redkapqvai
     1381 legapdaegi leeiqlqlap giqitglnps tqtvqidatf lqqlqqlqqq gninisitps
     1441 ttvsngadle qqldagsfli qqdatgsssa vldqslplva segkdgavtf tvveggssst
     1501 vqeeipdvqv aaaelgldel aaaegslmds avmdaadfvt pgiiteaage rrkkpvddgd
     1561 garvhvcdlc sksykrashl kehleshksk edkgkkalhq clecpkafsk psqlkrheri
     1621 htgerpfqct lcsksfnqnn alfvhlikht gakpfkcdac gsqftqkcnl mahirrvhkv
     1681 deaaskepet
//
LOCUS       XP_077503235            1690 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113786 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503235
VERSION     XP_077503235.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647109.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1690
                     /product="uncharacterized protein LOC144113786 isoform X4"
                     /calculated_mol_wt=185348
     Region          163..183
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(163,166,179,183)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(168,170,172,174..175,178..179,182,196,198,202..203,
                     206..207,210,224,226,228,230..231,234..235,238)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          191..211
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          219..239
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          247..263
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          279..299
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(279,282,295,299)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          322..>370
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          324..345
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          324..344
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(324,327,340,344)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(329,331,333,335..336,339..340,343,357,359,363..364,
                     367..368,371,385,387,389,391..392,395..396,400)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Site            order(329,335,342,344,351,358)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          348..787
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          352..372
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          352..370
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          380..401
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          415..436
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          575..595
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(575,578,591,595)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(580,582,584,586..587,590..591,594,608,610,614..615,
                     618..619,623,637,639,641,643..644,647..648,651)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          603..624
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          632..652
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          743..763
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(743,746,759,763)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(748,750,752,754..755,758..759,762,776,778,782..783,
                     786..787,790,804,806,808,810..811,814..815,818)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          771..791
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          797..819
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          799..819
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          811..836
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          827..847
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1063..>1337
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          1069..1089
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1101..1121
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1132..1152
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1252..1272
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1252,1255,1268,1272)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1257,1259,1261,1263..1264,1267..1268,1271,1285,1287,
                     1291..1292,1295..1296,1299,1313,1315,1317,1319..1320,
                     1323..1324,1327)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1264..1288
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1280..1300
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1308..1328
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1320..1345
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1336..1356
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1567..1587
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1567,1570,1583,1587)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          <1589..>1667
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          1601..1621
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1601,1604,1617,1621)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1606,1608,1610,1612..1613,1616..1617,1620,1634,1636,
                     1640..1641,1644..1645,1648,1662,1664,1666,1668..1669,
                     1672..1673)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1614..1638
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1629..1649
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1657..1675
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1690
                     /gene="LOC144113786"
                     /coded_by="XM_077647109.1:1010..6082"
                     /db_xref="GeneID:144113786"
ORIGIN      
        1 meiqdvrsit engiladasa llteaspget avaalpvvnl sipflggtqg imigttadgs
       61 gafvvdashl sllgssgvft dgvlqfsvqg nddaeqvvll qpqemanleg sstaplmels
      121 pnipavvppl aalpdaeaar kspridhkpd eigppvgkgp fkcsicsaef pkwsqvqrhe
      181 rahaedkpfr csrceasfnq elnlrlhmat hpgpgqpscp ecgksfarla slkahlmlhq
      241 keenlicaec gdefstqarl drhaqehkde lsrvkyyfcr qcpqkfskqp llkehmkmhy
      301 kikaslshrt ykrnidrssf shkcttckkq fqkpsqllrh irihtgerpf gcnichktfn
      361 qkgalqihls khsglrphrc efcsaafsqr gnlrahiqrv htgpsndqna ivfrcqectc
      421 tfrrlgslna hmsrvhsgqm klstsvsrke aletlsktlh qqasvvvdnt ldekdvldrs
      481 nadllqqvlv nsglthspst nnseakklpp ppeetvpsdm kensfvppsn pieaaiekva
      541 sttesamvms vadtatgqik mhiyrmvgnv rwhqcmyctk efkkpsdlvr hirihtqekp
      601 ykcshcfraf avkstltaht rathlgvkqf qcptcqhlfs trgslkvhmr ihtgdkpytc
      661 gicnksfsss grckmhvaah greadgfrlp snirgatgvi sedlssfipi qepifiseaa
      721 geaaiddgsa rrqlttqerp ykcsmclvgf kksshlkqhi rshtgekpfh ctecqrsfvs
      781 ngvlkahvkt htgireykca vcnatfttng slkrhmctht narpfmcpyc qktfktsvnc
      841 kkhmkihrge lilkrsdpqt qgaevdgttg avlsdslvea lvpvnpdevm gqnhngldat
      901 ipaaevlaqg sslehgdpdg fvaqevlvqd nlqqlqgtlf ggqsdqmdlg qaaqvfsqng
      961 ffsqtlpqlt lqgdpldvgt lassfvtssl aaptdlaapt elaaptelaa ptelapatse
     1021 sdelvaelpv snedvspsla qestlqqaaq dalrgaevda gdgekpfvct lcdkrfkrsa
     1081 ylkshmrchs vrmasqknhi cqqcgscfps afslkrhsdt hsegllcesf vceacsislp
     1141 naaqyqqhls lhqssdkekp tddlpsvdpd paaavdesqr tsghvvdkas vsatsrkfvt
     1201 ltdeqsrela rkdprestls vseraliata aekdresqew ekppaadypn rcemcpksfr
     1261 kpsdlarhvr ihtgerpfsc eicqksftvk stldthrrth rgernypchi cssffstmgs
     1321 lkvhmrlhtg srpfkcphcd lrfrtsghrk shilthfksg gvrrrsrmlt redkapqvai
     1381 legapdaegi leeiqlqlap giqitglnps tqtvqidatf lqqlqqlqqq gninisitps
     1441 ttvsngadle qqldagsfli qqdatgsssa vldqslplva segkdgavtf tvveggssst
     1501 vqeeipdvqv aaaelgldel aaaegslmds avmdaadfvt pgiiteaage rrkkpvddgd
     1561 garvhvcdlc sksykrashl kehleshksk edkgkkalhq clecpkafsk psqlkrheri
     1621 htgerpfqct lcsksfnqnn alfvhlikht gakpfkcdac gsqftqkcnl mahirrvhkv
     1681 deaaskepet
//
LOCUS       XP_077503236            1690 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113786 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503236
VERSION     XP_077503236.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647110.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1690
                     /product="uncharacterized protein LOC144113786 isoform X4"
                     /calculated_mol_wt=185348
     Region          163..183
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(163,166,179,183)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(168,170,172,174..175,178..179,182,196,198,202..203,
                     206..207,210,224,226,228,230..231,234..235,238)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          191..211
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          219..239
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          247..263
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          279..299
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(279,282,295,299)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          322..>370
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          324..345
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          324..344
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(324,327,340,344)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(329,331,333,335..336,339..340,343,357,359,363..364,
                     367..368,371,385,387,389,391..392,395..396,400)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Site            order(329,335,342,344,351,358)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          348..787
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          352..372
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          352..370
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          380..401
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          415..436
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          575..595
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(575,578,591,595)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(580,582,584,586..587,590..591,594,608,610,614..615,
                     618..619,623,637,639,641,643..644,647..648,651)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          603..624
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          632..652
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          743..763
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(743,746,759,763)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(748,750,752,754..755,758..759,762,776,778,782..783,
                     786..787,790,804,806,808,810..811,814..815,818)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          771..791
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          797..819
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          799..819
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          811..836
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          827..847
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1063..>1337
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          1069..1089
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1101..1121
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1132..1152
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1252..1272
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1252,1255,1268,1272)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1257,1259,1261,1263..1264,1267..1268,1271,1285,1287,
                     1291..1292,1295..1296,1299,1313,1315,1317,1319..1320,
                     1323..1324,1327)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1264..1288
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1280..1300
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1308..1328
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1320..1345
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1336..1356
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1567..1587
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1567,1570,1583,1587)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          <1589..>1667
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          1601..1621
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1601,1604,1617,1621)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1606,1608,1610,1612..1613,1616..1617,1620,1634,1636,
                     1640..1641,1644..1645,1648,1662,1664,1666,1668..1669,
                     1672..1673)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1614..1638
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1629..1649
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1657..1675
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1690
                     /gene="LOC144113786"
                     /coded_by="XM_077647110.1:274..5346"
                     /db_xref="GeneID:144113786"
ORIGIN      
        1 meiqdvrsit engiladasa llteaspget avaalpvvnl sipflggtqg imigttadgs
       61 gafvvdashl sllgssgvft dgvlqfsvqg nddaeqvvll qpqemanleg sstaplmels
      121 pnipavvppl aalpdaeaar kspridhkpd eigppvgkgp fkcsicsaef pkwsqvqrhe
      181 rahaedkpfr csrceasfnq elnlrlhmat hpgpgqpscp ecgksfarla slkahlmlhq
      241 keenlicaec gdefstqarl drhaqehkde lsrvkyyfcr qcpqkfskqp llkehmkmhy
      301 kikaslshrt ykrnidrssf shkcttckkq fqkpsqllrh irihtgerpf gcnichktfn
      361 qkgalqihls khsglrphrc efcsaafsqr gnlrahiqrv htgpsndqna ivfrcqectc
      421 tfrrlgslna hmsrvhsgqm klstsvsrke aletlsktlh qqasvvvdnt ldekdvldrs
      481 nadllqqvlv nsglthspst nnseakklpp ppeetvpsdm kensfvppsn pieaaiekva
      541 sttesamvms vadtatgqik mhiyrmvgnv rwhqcmyctk efkkpsdlvr hirihtqekp
      601 ykcshcfraf avkstltaht rathlgvkqf qcptcqhlfs trgslkvhmr ihtgdkpytc
      661 gicnksfsss grckmhvaah greadgfrlp snirgatgvi sedlssfipi qepifiseaa
      721 geaaiddgsa rrqlttqerp ykcsmclvgf kksshlkqhi rshtgekpfh ctecqrsfvs
      781 ngvlkahvkt htgireykca vcnatfttng slkrhmctht narpfmcpyc qktfktsvnc
      841 kkhmkihrge lilkrsdpqt qgaevdgttg avlsdslvea lvpvnpdevm gqnhngldat
      901 ipaaevlaqg sslehgdpdg fvaqevlvqd nlqqlqgtlf ggqsdqmdlg qaaqvfsqng
      961 ffsqtlpqlt lqgdpldvgt lassfvtssl aaptdlaapt elaaptelaa ptelapatse
     1021 sdelvaelpv snedvspsla qestlqqaaq dalrgaevda gdgekpfvct lcdkrfkrsa
     1081 ylkshmrchs vrmasqknhi cqqcgscfps afslkrhsdt hsegllcesf vceacsislp
     1141 naaqyqqhls lhqssdkekp tddlpsvdpd paaavdesqr tsghvvdkas vsatsrkfvt
     1201 ltdeqsrela rkdprestls vseraliata aekdresqew ekppaadypn rcemcpksfr
     1261 kpsdlarhvr ihtgerpfsc eicqksftvk stldthrrth rgernypchi cssffstmgs
     1321 lkvhmrlhtg srpfkcphcd lrfrtsghrk shilthfksg gvrrrsrmlt redkapqvai
     1381 legapdaegi leeiqlqlap giqitglnps tqtvqidatf lqqlqqlqqq gninisitps
     1441 ttvsngadle qqldagsfli qqdatgsssa vldqslplva segkdgavtf tvveggssst
     1501 vqeeipdvqv aaaelgldel aaaegslmds avmdaadfvt pgiiteaage rrkkpvddgd
     1561 garvhvcdlc sksykrashl kehleshksk edkgkkalhq clecpkafsk psqlkrheri
     1621 htgerpfqct lcsksfnqnn alfvhlikht gakpfkcdac gsqftqkcnl mahirrvhkv
     1681 deaaskepet
//
LOCUS       XP_077503237             357 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113788 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503237
VERSION     XP_077503237.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647111.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..357
                     /product="uncharacterized protein LOC144113788 isoform X1"
                     /calculated_mol_wt=40474
     Region          151..349
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(206,245,259,261)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..357
                     /gene="LOC144113788"
                     /coded_by="XM_077647111.1:709..1782"
                     /db_xref="GeneID:144113788"
ORIGIN      
        1 msfnlkrstv dpastqhprp trkvkhfask eeladhikks pygelfsipg tllhntlttt
       61 rreiqqpsqa pasvqrqqat hhsekvifqs pkckerqlnk veppvddfei tlerplcnln
      121 fgkllgsera vdklsltmts akeieaatre qrncaewfsh rkgritaslf kdvcrskrln
      181 ckslmkriln dncitslamq yginyeqlak qhalaafqtk htncrledcg lmihprypyl
      241 gcspdgllvc dchppallev kclyslwhvh pdelikegqc kadfcldsag vlkaahkyyy
      301 qvqaqlhlnl vgstvcylyl hvdqggalip vkrdegfmet hinnlesffe simlpkl
//
LOCUS       XP_077503238             179 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113788 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503238
VERSION     XP_077503238.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647112.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..179
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..179
                     /product="uncharacterized protein LOC144113788 isoform X2"
                     /calculated_mol_wt=20350
     CDS             1..179
                     /gene="LOC144113788"
                     /coded_by="XM_077647112.1:709..1248"
                     /db_xref="GeneID:144113788"
ORIGIN      
        1 msfnlkrstv dpastqhprp trkvkhfask eeladhikks pygelfsipg tllhntlttt
       61 rreiqqpsqa pasvqrqqat hhsekvifqs pkckerqlnk veppvddfei tlerplcnln
      121 fgkllgsera vdklsltmts akeieaatre qrncaewfsh rkgggprssr irrlrpwmt
//
LOCUS       XP_077503239             493 aa            linear   INV 25-NOV-2025
DEFINITION  pseudouridylate synthase RPUSD2-like [Amblyomma americanum].
ACCESSION   XP_077503239
VERSION     XP_077503239.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647113.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..493
                     /product="pseudouridylate synthase RPUSD2-like"
                     /calculated_mol_wt=56115
     Region          161..343
                     /region_name="PseudoU_synth_ScRIB2"
                     /note="Pseudouridine synthases similar to Saccharomyces
                     cerevisiae RIB2; cd02557"
                     /db_xref="CDD:211331"
     Site            order(223..226,325)
                     /site_type="active"
                     /note="probable active site [active]"
                     /db_xref="CDD:211331"
     CDS             1..493
                     /gene="LOC144113789"
                     /coded_by="XM_077647113.1:2639..4120"
                     /db_xref="GeneID:144113789"
ORIGIN      
        1 mlvvtteric ltrgnlgrsl rnlisavmst apkeslsdvv eeadigcgvk rkaeepitgl
       61 dikkirqgqk elrpgfgrdr fdeteyyfqh glrkvypyyf tyttfckgrw vghllvdvft
      121 refrahsrev yenaikgglv tvnsrpvgtd yrlkdndlla ntlhrhevpv lgapikilhn
      181 shdmvvidkp psipvhpcgr yrhnsvifil akehglqnlh tvhrldrlts glllmsrsvd
      241 rareleneik grqvskeyvc rvegcfpddv vtccepievi shkigvcgvg ahgkesktef
      301 kklsyngkss vvlckpltgr mhqirvhlqf lgypivndpl ynhtvfgsrk akggltektk
      361 eqlvedllel hnlkkwlgpe mdltldgylp eeegdgtsda daleepgash fpgslnhyle
      421 gdesaafram mekqafdkvk fvrrqdcqec rckyrdpepk dlliylhclr ytgpnwtyea
      481 pwpawarenw idd
//
LOCUS       XP_077503240            1451 aa            linear   INV 25-NOV-2025
DEFINITION  cleavage and polyadenylation specificity factor subunit 1 isoform
            X1 [Amblyomma americanum].
ACCESSION   XP_077503240
VERSION     XP_077503240.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647114.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1451
                     /product="cleavage and polyadenylation specificity factor
                     subunit 1 isoform X1"
                     /calculated_mol_wt=163899
     Region          17..1445
                     /region_name="SFT1"
                     /note="Pre-mRNA cleavage and polyadenylation specificity
                     factor [RNA processing and modification]; COG5161"
                     /db_xref="CDD:227490"
     CDS             1..1451
                     /gene="Cpsf160"
                     /coded_by="XM_077647114.1:149..4504"
                     /db_xref="GeneID:144113790"
ORIGIN      
        1 mdtikhfeer vkmstsmyav fkqthpptgv ehsvycnfyn lrerslviag ssvlkvfrlv
       61 pesdyaeqkh rvklehmhtf nlfgnvicle vvrlstarda lllcfrdakl slveydpsth
      121 dlktvciksf ekeefkagyv qtaqlpmirv dpcnrcaaml vysrtvavip frkdaateev
      181 dsgiafgnkp plldwypvpl teleekvnnv idmqflhgyy eptllilyep lrtwpgriai
      241 rqdtccilal slnlqqrvhp viwsftnlpf dclrvlavpr plggvlimav dsllylnqsv
      301 ppygvslnsf tdfstsfplk pqegvkisld caqacflnyd rlvlslkgge lyvltlfndg
      361 mrsvrnfyfd kaaasvltts mtlcedgylf lgsrlgnsll lhytekaaev ddvvkrdekt
      421 ecdatdgpps kkkrmdaigd wmasdvalid pdelevygse tmatkqltsy tfevcdslin
      481 igpcgkicmg epaflseefv qntdpdlelv ttagygknga lcvlqrsvrp qvvttfelpg
      541 clhmwtvmgp psekkpqekk eipdvpasee ktaeqppant haflilsrad ssmilqtdqe
      601 ineldhsgfs tqnptvfagn lgdgryvlqv cpmgvrlldg trqlqhipld vgssivsgsl
      661 adphviirsa eglvihltlr gdpatgcrla vlrpqltavk akilsicvyk dvsglfttqy
      721 repdeatkpq kpvpppkesl dtstnglldd edellygese enpilrepvr aeskdtnset
      781 dpvfeikeva ptfwlfvare ngvleiyslp dyklcflvkn fpmgqkvlvd svqmtapsgt
      841 ksseklsdis hesmpivhei lvvglgirqs rpllmarvde dlliyeafpf yetqrdghlk
      901 lrfkklshei flrerkyksq kpeneeeeka fqsrqwlqpf sdisgysgvf lcgyrpywlf
      961 mssrgelrch pmfvdgpiyc fapfhnvncp kgflhfnkqg elristlpth ltydapwpvr
     1021 kvplrctphf vnyhvdskty cvvtsqpdpc nhlvrftgde keyellerda ryifptmekf
     1081 slqllspvsw etipntrvdl dewehltcik nvmlssegts tgmkgylalg tnycygedvt
     1141 srgriiildi idvvpepgqp ltknkikivy skeqkgpvta lsqvvgflls aigqkiyiwq
     1201 lkdnelvgva fidtqiyihs vvtvknlilv gdvfksvsll ryqeasrtls lvsrdvrple
     1261 vyaveffids tqmsflvtda ernmllymyq pesrescggq rllrrgdfhv gspvaslfri
     1321 kcrmgdiaky drraaatvdg rhitmfatld gslgyvlpvp ektyrrllml qnvlvtniph
     1381 yaglnpkafr myhsqrrflg nphknildge liwkfmhlsf terselskki gttvtqitdd
     1441 lleietytah f
//
LOCUS       XP_077503241            1375 aa            linear   INV 25-NOV-2025
DEFINITION  cleavage and polyadenylation specificity factor subunit 1 isoform
            X2 [Amblyomma americanum].
ACCESSION   XP_077503241
VERSION     XP_077503241.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647115.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1375
                     /product="cleavage and polyadenylation specificity factor
                     subunit 1 isoform X2"
                     /calculated_mol_wt=154990
     Region          17..1369
                     /region_name="SFT1"
                     /note="Pre-mRNA cleavage and polyadenylation specificity
                     factor [RNA processing and modification]; COG5161"
                     /db_xref="CDD:227490"
     CDS             1..1375
                     /gene="Cpsf160"
                     /coded_by="XM_077647115.1:123..4250"
                     /db_xref="GeneID:144113790"
ORIGIN      
        1 mhtfnlfgnv iclevvrlst ardalllcfr daklslveyd psthdlktvc iksfekeefk
       61 agyvqtaqlp mirvdpcnrc aamlvysrtv avipfrkdaa teevdsgiaf gnkpplldwy
      121 pvplteleek vnnvidmqfl hgyyeptlli lyeplrtwpg riairqdtcc ilalslnlqq
      181 rvhpviwsft nlpfdclrvl avprplggvl imavdsllyl nqsvppygvs lnsftdfsts
      241 fplkpqegvk isldcaqacf lnydrlvlsl kggelyvltl fndgmrsvrn fyfdkaaasv
      301 lttsmtlced gylflgsrlg nslllhytek aaevddvvkr dektecdatd gppskkkrmd
      361 aigdwmasdv alidpdelev ygsetmatkq ltsytfevcd slinigpcgk icmgepafls
      421 eefvqntdpd lelvttagyg kngalcvlqr svrpqvvttf elpgclhmwt vmgppsekkp
      481 qekkeipdvp aseektaeqp panthaflil sradssmilq tdqeineldh sgfstqnptv
      541 fagnlgdgry vlqvcpmgvr lldgtrqlqh ipldvgssiv sgsladphvi irsaeglvih
      601 ltlrgdpatg crlavlrpql tavkakilsi cvykdvsglf ttqyrepdea tkpqkpvppp
      661 kesldtstng llddedelly geseenpilr epvraeskdt nsetdpvfei kevaptfwlf
      721 varengvlei yslpdyklcf lvknfpmgqk vlvdsvqmta psgtkssekl sdishesmpi
      781 vheilvvglg irqsrpllma rvdedlliye afpfyetqrd ghlklrfkkl sheiflrerk
      841 yksqkpenee eekafqsrqw lqpfsdisgy sgvflcgyrp ywlfmssrge lrchpmfvdg
      901 piycfapfhn vncpkgflhf nkqgelrist lpthltydap wpvrkvplrc tphfvnyhvd
      961 sktycvvtsq pdpcnhlvrf tgdekeyell erdaryifpt mekfslqlls pvswetipnt
     1021 rvdldewehl tciknvmlss egtstgmkgy lalgtnycyg edvtsrgrii ildiidvvpe
     1081 pgqpltknki kivyskeqkg pvtalsqvvg fllsaigqki yiwqlkdnel vgvafidtqi
     1141 yihsvvtvkn lilvgdvfks vsllryqeas rtlslvsrdv rplevyavef fidstqmsfl
     1201 vtdaernmll ymyqpesres cggqrllrrg dfhvgspvas lfrikcrmgd iakydrraaa
     1261 tvdgrhitmf atldgslgyv lpvpektyrr llmlqnvlvt niphyaglnp kafrmyhsqr
     1321 rflgnphkni ldgeliwkfm hlsftersel skkigttvtq itddlleiet ytahf
//
LOCUS       XP_077503242            1287 aa            linear   INV 25-NOV-2025
DEFINITION  cleavage and polyadenylation specificity factor subunit 1 isoform
            X3 [Amblyomma americanum].
ACCESSION   XP_077503242
VERSION     XP_077503242.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647116.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1287
                     /product="cleavage and polyadenylation specificity factor
                     subunit 1 isoform X3"
                     /calculated_mol_wt=144906
     Region          17..1276
                     /region_name="SFT1"
                     /note="Pre-mRNA cleavage and polyadenylation specificity
                     factor [RNA processing and modification]; COG5161"
                     /db_xref="CDD:227490"
     CDS             1..1287
                     /gene="Cpsf160"
                     /coded_by="XM_077647116.1:149..4012"
                     /db_xref="GeneID:144113790"
ORIGIN      
        1 mdtikhfeer vkmstsmyav fkqthpptgv ehsvycnfyn lrerslviag ssvlkvfrlv
       61 pesdyaeqkh rvklehmhtf nlfgnvicle vvrlstarda lllcfrdakl slveydpsth
      121 dlktvciksf ekeefkagyv qtaqlpmirv dpcnrcaaml vysrtvavip frkdaateev
      181 dsgiafgnkp plldwypvpl teleekvnnv idmqflhgyy eptllilyep lrtwpgriai
      241 rqdtccilal slnlqqrvhp viwsftnlpf dclrvlavpr plggvlimav dsllylnqsv
      301 ppygvslnsf tdfstsfplk pqegvkisld caqacflnyd rlvlslkgge lyvltlfndg
      361 mrsvrnfyfd kaaasvltts mtlcedgylf lgsrlgnsll lhytekaaev ddvvkrdekt
      421 ecdatdgpps kkkrmdaigd wmasdvalid pdelevygse tmatkqltsy tfevcdslin
      481 igpcgkicmg epaflseefv qntdpdlelv ttagygknga lcvlqrsvrp qvvttfelpg
      541 clhmwtvmgp psekkpqekk eipdvpasee ktaeqppant haflilsrad ssmilqtdqe
      601 ineldhsgfs tqnptvfagn lgdgryvlqv cpmgvrlldg trqlqhipld vgssivsgsl
      661 adphviirsa eglvihltlr gdpatgcrla vlrpqltavk akilsicvyk dvsglfttqy
      721 repdeatkpq kpvpppkesl dtstnglldd edellygese enpilrepvr aeskdtnset
      781 dpvfeikeva ptfwlfvare ngvleiyslp dyklcflvkn fpmgqkvlvd svqmtapsgt
      841 ksseklsdis hesmpivhei lvvglgirqs rpllmarvde dlliyeafpf yetqrdghlk
      901 lrfkklshei flrerkyksq kpeneeeeka fqsrqwlqpf sdisgysgvf lcgyrpywlf
      961 mssrgelrch pmfvdgpiyc fapfhnvncp kgflhfnkqg elristlpth ltydapwpvr
     1021 kvplrctphf vnyhvdskty cvvtsqpdpc nhlvrftgde keyellerda ryifptmekf
     1081 slqllspvsw etipntrvdl dewehltcik nvmlssegts tgmkgylalg tnycygedvt
     1141 srgriiildi idvvpepgqp ltknkikivy skeqkgpvta lsqvvgflls aigqkiyiwq
     1201 lkdnelvgva fidtqiyihs vvtvknlilv gdvfksvsll ryqeasrtls lvsrdvrple
     1261 vyaveffids tqmsflgpll clqcllt
//
LOCUS       XP_077503243             313 aa            linear   INV 25-NOV-2025
DEFINITION  regulatory particle non-ATPase 8 [Amblyomma americanum].
ACCESSION   XP_077503243
VERSION     XP_077503243.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647117.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..313
                     /product="regulatory particle non-ATPase 8"
                     /calculated_mol_wt=35276
     Region          10..290
                     /region_name="MPN_RPN7_8"
                     /note="Mpr1p, Pad1p N-terminal (MPN) domains without
                     catalytic isopeptidase activity, found in 19S proteasomal
                     subunits Rpn7 and Rpn8; cd08062"
                     /db_xref="CDD:163693"
     CDS             1..313
                     /gene="Rpn8"
                     /coded_by="XM_077647117.1:117..1058"
                     /db_xref="GeneID:144113791"
ORIGIN      
        1 mtgtdhpvsk vvvhplvlls vvdhfnrmgk tghqdrvvgi llgswkekgv ldvsnsfavp
       61 fdedekdktv wfldhdylen mysmfkkvna rerivgwyht gpnlhpsdip inelvrrycs
      121 tsvlviidaq llsiglptea yiaveevhdd gtpttktfeh vpseigaeea eevgvehllr
      181 dikdttlgtl sqritnqlmg lkglqgqvhh irdyleqvvm gtlpvnhtii yqlqdifnll
      241 pnvglqdfvk slyiktndqm lvvylaslvr svvalhnlin nkitnrdaek kesakkdepk
      301 dkkpeekkke vka
//
LOCUS       XP_077503244             924 aa            linear   INV 25-NOV-2025
DEFINITION  coatomer subunit beta' [Amblyomma americanum].
ACCESSION   XP_077503244
VERSION     XP_077503244.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647118.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..924
                     /product="coatomer subunit beta'"
                     /calculated_mol_wt=104054
     Region          15..297
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cd00200"
                     /db_xref="CDD:238121"
     Region          20..55
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Site            order(32,36,42..43,55..56,74,78,84..85,97..98,115,120,
                     126..127,141,159,164,170..171,183..184,204,208,214..215,
                     227..228,245,250,256..257,269..270,288,292)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          62..97
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          103..140
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          145..182
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          189..226
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          232..268
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          304..778
                     /region_name="Coatomer_WDAD_beta-like"
                     /note="Coatomer WD Associated Region from Coatomer Subunit
                     Beta and Beta'; cd22947"
                     /db_xref="CDD:438572"
     Region          304..347
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:438572"
     Region          353..387
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:438572"
     Region          393..425
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:438572"
     Region          431..462
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:438572"
     Region          466..519
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:438572"
     Region          523..562
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:438572"
     Site            order(556,586,705..707,734..735,756..757,760)
                     /site_type="other"
                     /note="coatomer beta'-subunit binding site [polypeptide
                     binding]"
                     /db_xref="CDD:438572"
     Region          563..587
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:438572"
     CDS             1..924
                     /gene="beta'COP"
                     /coded_by="XM_077647118.1:139..2913"
                     /db_xref="GeneID:144113792"
ORIGIN      
        1 mplkldvkrk llarsdrvkc vdlhptepwm laslyngnvh vwncesqqlv ktfevcdlpv
       61 raakfvprkn wvitgsddmq vrvfnyntle rvhmfeahsd yirsivvhpt qpfiltssdd
      121 mliklwnwek qwactqvfeg hthyvmqivi npkdnntfas asldrtvkvw qlgsvtpnft
      181 leghekgvnc vdyyhggdkp ylisgaddrl vkiwdyqnkt cvqtleghaq nitavcfhpe
      241 lpiimsgsed gtvriwhant yrlestlnyg lervwticsl qgsnnmalgy degsiiiklg
      301 reepamsmdn sgkiiwakhs eiqqanlkam tdteikdger lplqvkdmgs ceiypqtish
      361 npngrfvvvc gdgeyiiyta malrnksfgs aqefvwalds seyavrengs tvkifknfke
      421 rkafkpefga egifggfmlg vrsvsglafy dweslelvrr idiqpkhvyw sengelvsia
      481 tedsfyilky dqdavtkare akegvtedgi eeafdvlgev qesvktglwv gdcfiytnsv
      541 nrlnyyvgge ivtiahldrt myvlgyiske srlflgdkel nvvsysllls vleyqtavmr
      601 rdfetadkvl ptipkeqrtr vahflekqgf kaqalavstd pehrfelclq lgdtktahql
      661 aveaqseqkw kqlaelalah gdfslaqecl hnaldfagll llatsasnad misklatsae
      721 avgknniafl akfllgdaek afevllatrr ypeaaffakc yvpsqtsrav klwkeqlaki
      781 seksaqalad pedyenlfpg lqdsvkteef lkqqhlagrk pasdfpnivp lqdrnpveem
      841 kaaesagtfv ytvhateatg pvqpllctgk qqdiasvvee eagaqneeyv dldqleqele
      901 hdienlvlde nvdyklqedl lsdd
//
LOCUS       XP_077503245             940 aa            linear   INV 25-NOV-2025
DEFINITION  WW domain containing E3 ubiquitin protein ligase suppressor of
            deltex isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503245
VERSION     XP_077503245.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647119.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..940
                     /product="WW domain containing E3 ubiquitin protein ligase
                     suppressor of deltex isoform X1"
                     /calculated_mol_wt=105645
     Region          10..132
                     /region_name="C2_E3_ubiquitin_ligase"
                     /note="C2 domain present in E3 ubiquitin ligase; cd04021"
                     /db_xref="CDD:175988"
     Region          110..>442
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     Region          376..405
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          <406..940
                     /region_name="HUL4"
                     /note="Ubiquitin-protein ligase [Posttranslational
                     modification, protein turnover, chaperones]; COG5021"
                     /db_xref="CDD:227354"
     CDS             1..940
                     /gene="Su(dx)"
                     /coded_by="XM_077647119.1:233..3055"
                     /db_xref="GeneID:144113793"
ORIGIN      
        1 msegsssgsl felnvtvvsa klnssgilfk pdpyvelsvd ggvpvkteys kstcnpkwde
       61 qfpvlvtpys klhfrvfnhn slmkdallge gclelyqvle kadgkldkvp qpleltcgsk
      121 pgrsysyllt ildgmnvdms ryppkegiat alsrreaaap kssssgsihq qhngsvsghl
      181 sgssadlgsr qwgtpagarv rvrkshnsse ppvttaaaag lassaaeaag ngptpsdqpa
      241 hqssqnstss gggarpdgts qvahiampss staaedpsql gvgsgsqpsp wsststmvir
      301 mdnsggeaga hwaasepsse gasvqqsrlr rtgvpaspcg rpsraapaag tatgtgptag
      361 tpstaaagts adeeqlppgw evrydqfnrk yyvdhntrst twerpqplpp gwemrrdnrg
      421 rvyyvdhntr tttwqrptae svrnyqhwqs tqaqamqqcq qrflyhtpvq vddddplgpl
      481 pegwekridp nnrvyfvnhk nkttqwedpr tqgkeeplpp gweikytanr vryfvdhnsk
      541 tttfkdprps pkgaygvpla yernfkwklt qfrylchcns lpshikitvs rgnifedsfg
      601 qimrvpphel rrrlfitfkg eegldyggia rewffllshe vlnpmyclfe yagknnyslq
      661 inpassvnpd hllyfrfigr fiamalfhgk fiysgftlpf ykrmlgkklt mkdiesidne
      721 fynsliwike nnieecslel yfsvdfevlg qiqshelkpg ggeirvteen kdeylrlmtd
      781 wrfsrgqeeq tksfldgfne vlplewlhyf derelelmlc gmqeididdw qrnsiyrhyt
      841 rnskqviwfw qfirdmdnek rarllqfvtg tcrvpvggfa elmgsngpqr fciekvgket
      901 wlprshtcfn rldlppyksy eqlvekltya ieetegfaqe
//
LOCUS       XP_077503246             940 aa            linear   INV 25-NOV-2025
DEFINITION  WW domain containing E3 ubiquitin protein ligase suppressor of
            deltex isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503246
VERSION     XP_077503246.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647120.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..940
                     /product="WW domain containing E3 ubiquitin protein ligase
                     suppressor of deltex isoform X1"
                     /calculated_mol_wt=105645
     Region          10..132
                     /region_name="C2_E3_ubiquitin_ligase"
                     /note="C2 domain present in E3 ubiquitin ligase; cd04021"
                     /db_xref="CDD:175988"
     Region          110..>442
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     Region          376..405
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          <406..940
                     /region_name="HUL4"
                     /note="Ubiquitin-protein ligase [Posttranslational
                     modification, protein turnover, chaperones]; COG5021"
                     /db_xref="CDD:227354"
     CDS             1..940
                     /gene="Su(dx)"
                     /coded_by="XM_077647120.1:220..3042"
                     /db_xref="GeneID:144113793"
ORIGIN      
        1 msegsssgsl felnvtvvsa klnssgilfk pdpyvelsvd ggvpvkteys kstcnpkwde
       61 qfpvlvtpys klhfrvfnhn slmkdallge gclelyqvle kadgkldkvp qpleltcgsk
      121 pgrsysyllt ildgmnvdms ryppkegiat alsrreaaap kssssgsihq qhngsvsghl
      181 sgssadlgsr qwgtpagarv rvrkshnsse ppvttaaaag lassaaeaag ngptpsdqpa
      241 hqssqnstss gggarpdgts qvahiampss staaedpsql gvgsgsqpsp wsststmvir
      301 mdnsggeaga hwaasepsse gasvqqsrlr rtgvpaspcg rpsraapaag tatgtgptag
      361 tpstaaagts adeeqlppgw evrydqfnrk yyvdhntrst twerpqplpp gwemrrdnrg
      421 rvyyvdhntr tttwqrptae svrnyqhwqs tqaqamqqcq qrflyhtpvq vddddplgpl
      481 pegwekridp nnrvyfvnhk nkttqwedpr tqgkeeplpp gweikytanr vryfvdhnsk
      541 tttfkdprps pkgaygvpla yernfkwklt qfrylchcns lpshikitvs rgnifedsfg
      601 qimrvpphel rrrlfitfkg eegldyggia rewffllshe vlnpmyclfe yagknnyslq
      661 inpassvnpd hllyfrfigr fiamalfhgk fiysgftlpf ykrmlgkklt mkdiesidne
      721 fynsliwike nnieecslel yfsvdfevlg qiqshelkpg ggeirvteen kdeylrlmtd
      781 wrfsrgqeeq tksfldgfne vlplewlhyf derelelmlc gmqeididdw qrnsiyrhyt
      841 rnskqviwfw qfirdmdnek rarllqfvtg tcrvpvggfa elmgsngpqr fciekvgket
      901 wlprshtcfn rldlppyksy eqlvekltya ieetegfaqe
//
LOCUS       XP_077503247             940 aa            linear   INV 25-NOV-2025
DEFINITION  WW domain containing E3 ubiquitin protein ligase suppressor of
            deltex isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503247
VERSION     XP_077503247.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647121.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..940
                     /product="WW domain containing E3 ubiquitin protein ligase
                     suppressor of deltex isoform X1"
                     /calculated_mol_wt=105645
     Region          10..132
                     /region_name="C2_E3_ubiquitin_ligase"
                     /note="C2 domain present in E3 ubiquitin ligase; cd04021"
                     /db_xref="CDD:175988"
     Region          110..>442
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     Region          376..405
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          <406..940
                     /region_name="HUL4"
                     /note="Ubiquitin-protein ligase [Posttranslational
                     modification, protein turnover, chaperones]; COG5021"
                     /db_xref="CDD:227354"
     CDS             1..940
                     /gene="Su(dx)"
                     /coded_by="XM_077647121.1:264..3086"
                     /db_xref="GeneID:144113793"
ORIGIN      
        1 msegsssgsl felnvtvvsa klnssgilfk pdpyvelsvd ggvpvkteys kstcnpkwde
       61 qfpvlvtpys klhfrvfnhn slmkdallge gclelyqvle kadgkldkvp qpleltcgsk
      121 pgrsysyllt ildgmnvdms ryppkegiat alsrreaaap kssssgsihq qhngsvsghl
      181 sgssadlgsr qwgtpagarv rvrkshnsse ppvttaaaag lassaaeaag ngptpsdqpa
      241 hqssqnstss gggarpdgts qvahiampss staaedpsql gvgsgsqpsp wsststmvir
      301 mdnsggeaga hwaasepsse gasvqqsrlr rtgvpaspcg rpsraapaag tatgtgptag
      361 tpstaaagts adeeqlppgw evrydqfnrk yyvdhntrst twerpqplpp gwemrrdnrg
      421 rvyyvdhntr tttwqrptae svrnyqhwqs tqaqamqqcq qrflyhtpvq vddddplgpl
      481 pegwekridp nnrvyfvnhk nkttqwedpr tqgkeeplpp gweikytanr vryfvdhnsk
      541 tttfkdprps pkgaygvpla yernfkwklt qfrylchcns lpshikitvs rgnifedsfg
      601 qimrvpphel rrrlfitfkg eegldyggia rewffllshe vlnpmyclfe yagknnyslq
      661 inpassvnpd hllyfrfigr fiamalfhgk fiysgftlpf ykrmlgkklt mkdiesidne
      721 fynsliwike nnieecslel yfsvdfevlg qiqshelkpg ggeirvteen kdeylrlmtd
      781 wrfsrgqeeq tksfldgfne vlplewlhyf derelelmlc gmqeididdw qrnsiyrhyt
      841 rnskqviwfw qfirdmdnek rarllqfvtg tcrvpvggfa elmgsngpqr fciekvgket
      901 wlprshtcfn rldlppyksy eqlvekltya ieetegfaqe
//
LOCUS       XP_077503248             940 aa            linear   INV 25-NOV-2025
DEFINITION  WW domain containing E3 ubiquitin protein ligase suppressor of
            deltex isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503248
VERSION     XP_077503248.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647122.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..940
                     /product="WW domain containing E3 ubiquitin protein ligase
                     suppressor of deltex isoform X1"
                     /calculated_mol_wt=105645
     Region          10..132
                     /region_name="C2_E3_ubiquitin_ligase"
                     /note="C2 domain present in E3 ubiquitin ligase; cd04021"
                     /db_xref="CDD:175988"
     Region          110..>442
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     Region          376..405
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          <406..940
                     /region_name="HUL4"
                     /note="Ubiquitin-protein ligase [Posttranslational
                     modification, protein turnover, chaperones]; COG5021"
                     /db_xref="CDD:227354"
     CDS             1..940
                     /gene="Su(dx)"
                     /coded_by="XM_077647122.1:113..2935"
                     /db_xref="GeneID:144113793"
ORIGIN      
        1 msegsssgsl felnvtvvsa klnssgilfk pdpyvelsvd ggvpvkteys kstcnpkwde
       61 qfpvlvtpys klhfrvfnhn slmkdallge gclelyqvle kadgkldkvp qpleltcgsk
      121 pgrsysyllt ildgmnvdms ryppkegiat alsrreaaap kssssgsihq qhngsvsghl
      181 sgssadlgsr qwgtpagarv rvrkshnsse ppvttaaaag lassaaeaag ngptpsdqpa
      241 hqssqnstss gggarpdgts qvahiampss staaedpsql gvgsgsqpsp wsststmvir
      301 mdnsggeaga hwaasepsse gasvqqsrlr rtgvpaspcg rpsraapaag tatgtgptag
      361 tpstaaagts adeeqlppgw evrydqfnrk yyvdhntrst twerpqplpp gwemrrdnrg
      421 rvyyvdhntr tttwqrptae svrnyqhwqs tqaqamqqcq qrflyhtpvq vddddplgpl
      481 pegwekridp nnrvyfvnhk nkttqwedpr tqgkeeplpp gweikytanr vryfvdhnsk
      541 tttfkdprps pkgaygvpla yernfkwklt qfrylchcns lpshikitvs rgnifedsfg
      601 qimrvpphel rrrlfitfkg eegldyggia rewffllshe vlnpmyclfe yagknnyslq
      661 inpassvnpd hllyfrfigr fiamalfhgk fiysgftlpf ykrmlgkklt mkdiesidne
      721 fynsliwike nnieecslel yfsvdfevlg qiqshelkpg ggeirvteen kdeylrlmtd
      781 wrfsrgqeeq tksfldgfne vlplewlhyf derelelmlc gmqeididdw qrnsiyrhyt
      841 rnskqviwfw qfirdmdnek rarllqfvtg tcrvpvggfa elmgsngpqr fciekvgket
      901 wlprshtcfn rldlppyksy eqlvekltya ieetegfaqe
//
LOCUS       XP_077503249             940 aa            linear   INV 25-NOV-2025
DEFINITION  WW domain containing E3 ubiquitin protein ligase suppressor of
            deltex isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503249
VERSION     XP_077503249.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647123.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..940
                     /product="WW domain containing E3 ubiquitin protein ligase
                     suppressor of deltex isoform X1"
                     /calculated_mol_wt=105645
     Region          10..132
                     /region_name="C2_E3_ubiquitin_ligase"
                     /note="C2 domain present in E3 ubiquitin ligase; cd04021"
                     /db_xref="CDD:175988"
     Region          110..>442
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     Region          376..405
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          <406..940
                     /region_name="HUL4"
                     /note="Ubiquitin-protein ligase [Posttranslational
                     modification, protein turnover, chaperones]; COG5021"
                     /db_xref="CDD:227354"
     CDS             1..940
                     /gene="Su(dx)"
                     /coded_by="XM_077647123.1:296..3118"
                     /db_xref="GeneID:144113793"
ORIGIN      
        1 msegsssgsl felnvtvvsa klnssgilfk pdpyvelsvd ggvpvkteys kstcnpkwde
       61 qfpvlvtpys klhfrvfnhn slmkdallge gclelyqvle kadgkldkvp qpleltcgsk
      121 pgrsysyllt ildgmnvdms ryppkegiat alsrreaaap kssssgsihq qhngsvsghl
      181 sgssadlgsr qwgtpagarv rvrkshnsse ppvttaaaag lassaaeaag ngptpsdqpa
      241 hqssqnstss gggarpdgts qvahiampss staaedpsql gvgsgsqpsp wsststmvir
      301 mdnsggeaga hwaasepsse gasvqqsrlr rtgvpaspcg rpsraapaag tatgtgptag
      361 tpstaaagts adeeqlppgw evrydqfnrk yyvdhntrst twerpqplpp gwemrrdnrg
      421 rvyyvdhntr tttwqrptae svrnyqhwqs tqaqamqqcq qrflyhtpvq vddddplgpl
      481 pegwekridp nnrvyfvnhk nkttqwedpr tqgkeeplpp gweikytanr vryfvdhnsk
      541 tttfkdprps pkgaygvpla yernfkwklt qfrylchcns lpshikitvs rgnifedsfg
      601 qimrvpphel rrrlfitfkg eegldyggia rewffllshe vlnpmyclfe yagknnyslq
      661 inpassvnpd hllyfrfigr fiamalfhgk fiysgftlpf ykrmlgkklt mkdiesidne
      721 fynsliwike nnieecslel yfsvdfevlg qiqshelkpg ggeirvteen kdeylrlmtd
      781 wrfsrgqeeq tksfldgfne vlplewlhyf derelelmlc gmqeididdw qrnsiyrhyt
      841 rnskqviwfw qfirdmdnek rarllqfvtg tcrvpvggfa elmgsngpqr fciekvgket
      901 wlprshtcfn rldlppyksy eqlvekltya ieetegfaqe
//
LOCUS       XP_077503250             940 aa            linear   INV 25-NOV-2025
DEFINITION  WW domain containing E3 ubiquitin protein ligase suppressor of
            deltex isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503250
VERSION     XP_077503250.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647124.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..940
                     /product="WW domain containing E3 ubiquitin protein ligase
                     suppressor of deltex isoform X1"
                     /calculated_mol_wt=105645
     Region          10..132
                     /region_name="C2_E3_ubiquitin_ligase"
                     /note="C2 domain present in E3 ubiquitin ligase; cd04021"
                     /db_xref="CDD:175988"
     Region          110..>442
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     Region          376..405
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          <406..940
                     /region_name="HUL4"
                     /note="Ubiquitin-protein ligase [Posttranslational
                     modification, protein turnover, chaperones]; COG5021"
                     /db_xref="CDD:227354"
     CDS             1..940
                     /gene="Su(dx)"
                     /coded_by="XM_077647124.1:223..3045"
                     /db_xref="GeneID:144113793"
ORIGIN      
        1 msegsssgsl felnvtvvsa klnssgilfk pdpyvelsvd ggvpvkteys kstcnpkwde
       61 qfpvlvtpys klhfrvfnhn slmkdallge gclelyqvle kadgkldkvp qpleltcgsk
      121 pgrsysyllt ildgmnvdms ryppkegiat alsrreaaap kssssgsihq qhngsvsghl
      181 sgssadlgsr qwgtpagarv rvrkshnsse ppvttaaaag lassaaeaag ngptpsdqpa
      241 hqssqnstss gggarpdgts qvahiampss staaedpsql gvgsgsqpsp wsststmvir
      301 mdnsggeaga hwaasepsse gasvqqsrlr rtgvpaspcg rpsraapaag tatgtgptag
      361 tpstaaagts adeeqlppgw evrydqfnrk yyvdhntrst twerpqplpp gwemrrdnrg
      421 rvyyvdhntr tttwqrptae svrnyqhwqs tqaqamqqcq qrflyhtpvq vddddplgpl
      481 pegwekridp nnrvyfvnhk nkttqwedpr tqgkeeplpp gweikytanr vryfvdhnsk
      541 tttfkdprps pkgaygvpla yernfkwklt qfrylchcns lpshikitvs rgnifedsfg
      601 qimrvpphel rrrlfitfkg eegldyggia rewffllshe vlnpmyclfe yagknnyslq
      661 inpassvnpd hllyfrfigr fiamalfhgk fiysgftlpf ykrmlgkklt mkdiesidne
      721 fynsliwike nnieecslel yfsvdfevlg qiqshelkpg ggeirvteen kdeylrlmtd
      781 wrfsrgqeeq tksfldgfne vlplewlhyf derelelmlc gmqeididdw qrnsiyrhyt
      841 rnskqviwfw qfirdmdnek rarllqfvtg tcrvpvggfa elmgsngpqr fciekvgket
      901 wlprshtcfn rldlppyksy eqlvekltya ieetegfaqe
//
LOCUS       XP_077503251             940 aa            linear   INV 25-NOV-2025
DEFINITION  WW domain containing E3 ubiquitin protein ligase suppressor of
            deltex isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503251
VERSION     XP_077503251.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647125.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..940
                     /product="WW domain containing E3 ubiquitin protein ligase
                     suppressor of deltex isoform X1"
                     /calculated_mol_wt=105645
     Region          10..132
                     /region_name="C2_E3_ubiquitin_ligase"
                     /note="C2 domain present in E3 ubiquitin ligase; cd04021"
                     /db_xref="CDD:175988"
     Region          110..>442
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     Region          376..405
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          <406..940
                     /region_name="HUL4"
                     /note="Ubiquitin-protein ligase [Posttranslational
                     modification, protein turnover, chaperones]; COG5021"
                     /db_xref="CDD:227354"
     CDS             1..940
                     /gene="Su(dx)"
                     /coded_by="XM_077647125.1:857..3679"
                     /db_xref="GeneID:144113793"
ORIGIN      
        1 msegsssgsl felnvtvvsa klnssgilfk pdpyvelsvd ggvpvkteys kstcnpkwde
       61 qfpvlvtpys klhfrvfnhn slmkdallge gclelyqvle kadgkldkvp qpleltcgsk
      121 pgrsysyllt ildgmnvdms ryppkegiat alsrreaaap kssssgsihq qhngsvsghl
      181 sgssadlgsr qwgtpagarv rvrkshnsse ppvttaaaag lassaaeaag ngptpsdqpa
      241 hqssqnstss gggarpdgts qvahiampss staaedpsql gvgsgsqpsp wsststmvir
      301 mdnsggeaga hwaasepsse gasvqqsrlr rtgvpaspcg rpsraapaag tatgtgptag
      361 tpstaaagts adeeqlppgw evrydqfnrk yyvdhntrst twerpqplpp gwemrrdnrg
      421 rvyyvdhntr tttwqrptae svrnyqhwqs tqaqamqqcq qrflyhtpvq vddddplgpl
      481 pegwekridp nnrvyfvnhk nkttqwedpr tqgkeeplpp gweikytanr vryfvdhnsk
      541 tttfkdprps pkgaygvpla yernfkwklt qfrylchcns lpshikitvs rgnifedsfg
      601 qimrvpphel rrrlfitfkg eegldyggia rewffllshe vlnpmyclfe yagknnyslq
      661 inpassvnpd hllyfrfigr fiamalfhgk fiysgftlpf ykrmlgkklt mkdiesidne
      721 fynsliwike nnieecslel yfsvdfevlg qiqshelkpg ggeirvteen kdeylrlmtd
      781 wrfsrgqeeq tksfldgfne vlplewlhyf derelelmlc gmqeididdw qrnsiyrhyt
      841 rnskqviwfw qfirdmdnek rarllqfvtg tcrvpvggfa elmgsngpqr fciekvgket
      901 wlprshtcfn rldlppyksy eqlvekltya ieetegfaqe
//
LOCUS       XP_077503252             940 aa            linear   INV 25-NOV-2025
DEFINITION  WW domain containing E3 ubiquitin protein ligase suppressor of
            deltex isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503252
VERSION     XP_077503252.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647126.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..940
                     /product="WW domain containing E3 ubiquitin protein ligase
                     suppressor of deltex isoform X1"
                     /calculated_mol_wt=105645
     Region          10..132
                     /region_name="C2_E3_ubiquitin_ligase"
                     /note="C2 domain present in E3 ubiquitin ligase; cd04021"
                     /db_xref="CDD:175988"
     Region          110..>442
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     Region          376..405
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          <406..940
                     /region_name="HUL4"
                     /note="Ubiquitin-protein ligase [Posttranslational
                     modification, protein turnover, chaperones]; COG5021"
                     /db_xref="CDD:227354"
     CDS             1..940
                     /gene="Su(dx)"
                     /coded_by="XM_077647126.1:255..3077"
                     /db_xref="GeneID:144113793"
ORIGIN      
        1 msegsssgsl felnvtvvsa klnssgilfk pdpyvelsvd ggvpvkteys kstcnpkwde
       61 qfpvlvtpys klhfrvfnhn slmkdallge gclelyqvle kadgkldkvp qpleltcgsk
      121 pgrsysyllt ildgmnvdms ryppkegiat alsrreaaap kssssgsihq qhngsvsghl
      181 sgssadlgsr qwgtpagarv rvrkshnsse ppvttaaaag lassaaeaag ngptpsdqpa
      241 hqssqnstss gggarpdgts qvahiampss staaedpsql gvgsgsqpsp wsststmvir
      301 mdnsggeaga hwaasepsse gasvqqsrlr rtgvpaspcg rpsraapaag tatgtgptag
      361 tpstaaagts adeeqlppgw evrydqfnrk yyvdhntrst twerpqplpp gwemrrdnrg
      421 rvyyvdhntr tttwqrptae svrnyqhwqs tqaqamqqcq qrflyhtpvq vddddplgpl
      481 pegwekridp nnrvyfvnhk nkttqwedpr tqgkeeplpp gweikytanr vryfvdhnsk
      541 tttfkdprps pkgaygvpla yernfkwklt qfrylchcns lpshikitvs rgnifedsfg
      601 qimrvpphel rrrlfitfkg eegldyggia rewffllshe vlnpmyclfe yagknnyslq
      661 inpassvnpd hllyfrfigr fiamalfhgk fiysgftlpf ykrmlgkklt mkdiesidne
      721 fynsliwike nnieecslel yfsvdfevlg qiqshelkpg ggeirvteen kdeylrlmtd
      781 wrfsrgqeeq tksfldgfne vlplewlhyf derelelmlc gmqeididdw qrnsiyrhyt
      841 rnskqviwfw qfirdmdnek rarllqfvtg tcrvpvggfa elmgsngpqr fciekvgket
      901 wlprshtcfn rldlppyksy eqlvekltya ieetegfaqe
//
LOCUS       XP_077503253             939 aa            linear   INV 25-NOV-2025
DEFINITION  WW domain containing E3 ubiquitin protein ligase suppressor of
            deltex isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503253
VERSION     XP_077503253.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647127.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..939
                     /product="WW domain containing E3 ubiquitin protein ligase
                     suppressor of deltex isoform X2"
                     /calculated_mol_wt=105574
     Region          10..132
                     /region_name="C2_E3_ubiquitin_ligase"
                     /note="C2 domain present in E3 ubiquitin ligase; cd04021"
                     /db_xref="CDD:175988"
     Region          110..>441
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     Region          375..404
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          <405..939
                     /region_name="HUL4"
                     /note="Ubiquitin-protein ligase [Posttranslational
                     modification, protein turnover, chaperones]; COG5021"
                     /db_xref="CDD:227354"
     CDS             1..939
                     /gene="Su(dx)"
                     /coded_by="XM_077647127.1:233..3052"
                     /db_xref="GeneID:144113793"
ORIGIN      
        1 msegsssgsl felnvtvvsa klnssgilfk pdpyvelsvd ggvpvkteys kstcnpkwde
       61 qfpvlvtpys klhfrvfnhn slmkdallge gclelyqvle kadgkldkvp qpleltcgsk
      121 pgrsysyllt ildgmnvdms ryppkegiat alsrreaapk ssssgsihqq hngsvsghls
      181 gssadlgsrq wgtpagarvr vrkshnssep pvttaaaagl assaaeaagn gptpsdqpah
      241 qssqnstssg ggarpdgtsq vahiampsss taaedpsqlg vgsgsqpspw sststmvirm
      301 dnsggeagah waasepsseg asvqqsrlrr tgvpaspcgr psraapaagt atgtgptagt
      361 pstaaagtsa deeqlppgwe vrydqfnrky yvdhntrstt werpqplppg wemrrdnrgr
      421 vyyvdhntrt ttwqrptaes vrnyqhwqst qaqamqqcqq rflyhtpvqv ddddplgplp
      481 egwekridpn nrvyfvnhkn kttqwedprt qgkeeplppg weikytanrv ryfvdhnskt
      541 ttfkdprpsp kgaygvplay ernfkwkltq frylchcnsl pshikitvsr gnifedsfgq
      601 imrvpphelr rrlfitfkge egldyggiar ewffllshev lnpmyclfey agknnyslqi
      661 npassvnpdh llyfrfigrf iamalfhgkf iysgftlpfy krmlgkkltm kdiesidnef
      721 ynsliwiken nieecslely fsvdfevlgq iqshelkpgg geirvteenk deylrlmtdw
      781 rfsrgqeeqt ksfldgfnev lplewlhyfd erelelmlcg mqeididdwq rnsiyrhytr
      841 nskqviwfwq firdmdnekr arllqfvtgt crvpvggfae lmgsngpqrf ciekvgketw
      901 lprshtcfnr ldlppyksye qlvekltyai eetegfaqe
//
LOCUS       XP_077503254             939 aa            linear   INV 25-NOV-2025
DEFINITION  WW domain containing E3 ubiquitin protein ligase suppressor of
            deltex isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503254
VERSION     XP_077503254.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647128.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..939
                     /product="WW domain containing E3 ubiquitin protein ligase
                     suppressor of deltex isoform X2"
                     /calculated_mol_wt=105574
     Region          10..132
                     /region_name="C2_E3_ubiquitin_ligase"
                     /note="C2 domain present in E3 ubiquitin ligase; cd04021"
                     /db_xref="CDD:175988"
     Region          110..>441
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     Region          375..404
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          <405..939
                     /region_name="HUL4"
                     /note="Ubiquitin-protein ligase [Posttranslational
                     modification, protein turnover, chaperones]; COG5021"
                     /db_xref="CDD:227354"
     CDS             1..939
                     /gene="Su(dx)"
                     /coded_by="XM_077647128.1:416..3235"
                     /db_xref="GeneID:144113793"
ORIGIN      
        1 msegsssgsl felnvtvvsa klnssgilfk pdpyvelsvd ggvpvkteys kstcnpkwde
       61 qfpvlvtpys klhfrvfnhn slmkdallge gclelyqvle kadgkldkvp qpleltcgsk
      121 pgrsysyllt ildgmnvdms ryppkegiat alsrreaapk ssssgsihqq hngsvsghls
      181 gssadlgsrq wgtpagarvr vrkshnssep pvttaaaagl assaaeaagn gptpsdqpah
      241 qssqnstssg ggarpdgtsq vahiampsss taaedpsqlg vgsgsqpspw sststmvirm
      301 dnsggeagah waasepsseg asvqqsrlrr tgvpaspcgr psraapaagt atgtgptagt
      361 pstaaagtsa deeqlppgwe vrydqfnrky yvdhntrstt werpqplppg wemrrdnrgr
      421 vyyvdhntrt ttwqrptaes vrnyqhwqst qaqamqqcqq rflyhtpvqv ddddplgplp
      481 egwekridpn nrvyfvnhkn kttqwedprt qgkeeplppg weikytanrv ryfvdhnskt
      541 ttfkdprpsp kgaygvplay ernfkwkltq frylchcnsl pshikitvsr gnifedsfgq
      601 imrvpphelr rrlfitfkge egldyggiar ewffllshev lnpmyclfey agknnyslqi
      661 npassvnpdh llyfrfigrf iamalfhgkf iysgftlpfy krmlgkkltm kdiesidnef
      721 ynsliwiken nieecslely fsvdfevlgq iqshelkpgg geirvteenk deylrlmtdw
      781 rfsrgqeeqt ksfldgfnev lplewlhyfd erelelmlcg mqeididdwq rnsiyrhytr
      841 nskqviwfwq firdmdnekr arllqfvtgt crvpvggfae lmgsngpqrf ciekvgketw
      901 lprshtcfnr ldlppyksye qlvekltyai eetegfaqe
//
LOCUS       XP_077503255             938 aa            linear   INV 25-NOV-2025
DEFINITION  WW domain containing E3 ubiquitin protein ligase suppressor of
            deltex isoform X3 [Amblyomma americanum].
ACCESSION   XP_077503255
VERSION     XP_077503255.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647129.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..938
                     /product="WW domain containing E3 ubiquitin protein ligase
                     suppressor of deltex isoform X3"
                     /calculated_mol_wt=105503
     Region          10..132
                     /region_name="C2_E3_ubiquitin_ligase"
                     /note="C2 domain present in E3 ubiquitin ligase; cd04021"
                     /db_xref="CDD:175988"
     Region          140..>342
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     Region          374..403
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          <404..938
                     /region_name="HUL4"
                     /note="Ubiquitin-protein ligase [Posttranslational
                     modification, protein turnover, chaperones]; COG5021"
                     /db_xref="CDD:227354"
     CDS             1..938
                     /gene="Su(dx)"
                     /coded_by="XM_077647129.1:233..3049"
                     /db_xref="GeneID:144113793"
ORIGIN      
        1 msegsssgsl felnvtvvsa klnssgilfk pdpyvelsvd ggvpvkteys kstcnpkwde
       61 qfpvlvtpys klhfrvfnhn slmkdallge gclelyqvle kadgkldkvp qpleltcgsk
      121 pgrsysyllt ildgmnvdms ryppkegiat alsrreapks sssgsihqqh ngsvsghlsg
      181 ssadlgsrqw gtpagarvrv rkshnssepp vttaaaagla ssaaeaagng ptpsdqpahq
      241 ssqnstssgg garpdgtsqv ahiampssst aaedpsqlgv gsgsqpspws ststmvirmd
      301 nsggeagahw aasepssega svqqsrlrrt gvpaspcgrp sraapaagta tgtgptagtp
      361 staaagtsad eeqlppgwev rydqfnrkyy vdhntrsttw erpqplppgw emrrdnrgrv
      421 yyvdhntrtt twqrptaesv rnyqhwqstq aqamqqcqqr flyhtpvqvd dddplgplpe
      481 gwekridpnn rvyfvnhknk ttqwedprtq gkeeplppgw eikytanrvr yfvdhnsktt
      541 tfkdprpspk gaygvplaye rnfkwkltqf rylchcnslp shikitvsrg nifedsfgqi
      601 mrvpphelrr rlfitfkgee gldyggiare wffllshevl npmyclfeya gknnyslqin
      661 passvnpdhl lyfrfigrfi amalfhgkfi ysgftlpfyk rmlgkkltmk diesidnefy
      721 nsliwikenn ieecslelyf svdfevlgqi qshelkpggg eirvteenkd eylrlmtdwr
      781 fsrgqeeqtk sfldgfnevl plewlhyfde relelmlcgm qeididdwqr nsiyrhytrn
      841 skqviwfwqf irdmdnekra rllqfvtgtc rvpvggfael mgsngpqrfc iekvgketwl
      901 prshtcfnrl dlppyksyeq lvekltyaie etegfaqe
//
LOCUS       XP_077503256             413 aa            linear   INV 25-NOV-2025
DEFINITION  RNA-binding protein NOB1 [Amblyomma americanum].
ACCESSION   XP_077503256
VERSION     XP_077503256.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647130.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..413
                     /product="RNA-binding protein NOB1"
                     /calculated_mol_wt=45245
     Region          47..133
                     /region_name="PIN_6"
                     /note="PIN domain of ribonuclease; pfam17146"
                     /db_xref="CDD:465362"
     Region          262..333
                     /region_name="NOB1_Zn_bind"
                     /note="Nin one binding (NOB1) Zn-ribbon like; pfam08772"
                     /db_xref="CDD:462597"
     CDS             1..413
                     /gene="LOC144113795"
                     /coded_by="XM_077647130.1:21..1262"
                     /db_xref="GeneID:144113795"
ORIGIN      
        1 mvvavrdggs celcelvfak avnlfrtcsl cgamepvmll garvkhlvvd sggfiknaal
       61 heigkeiytl pevvneikdk atkqrlqvlp ytinyrvpsp eavkiisdfs kltgdypsls
      121 avdikvlalt ymlekehvgt dhlckkpkcm evqgtgaprd avaspgffga ltieentqkp
      181 ksvepascad etsedeadla eedssdgewi tpgniteikk emglltleev pipvacistd
      241 favqnvliqm glkavsvdgm aikhartfvl rchacftitk vmtkqfcpac gnktlkrvsv
      301 sveedgstkl yinykrpinv rgtryslptp kggkhstdpi lcedqpvpqn rlskmamsqv
      361 dvldadylsr nspfkindvy srsahlnmra grqgsrnpnq vkrntgnhkk rsk
//
LOCUS       XP_077503257             779 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113796 [Amblyomma americanum].
ACCESSION   XP_077503257
VERSION     XP_077503257.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647131.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..779
                     /product="uncharacterized protein LOC144113796"
                     /calculated_mol_wt=82965
     CDS             1..779
                     /gene="LOC144113796"
                     /coded_by="XM_077647131.1:129..2468"
                     /db_xref="GeneID:144113796"
ORIGIN      
        1 mvckeeiysw fkdlngavri dtmcgllnlc lplelrflgt cvedlaskdy ayfreaelra
       61 nsfselskct svrdavtrsk litslallhl cnrscshaif qalseemkct kqetydtktl
      121 eemlllftma thhpsfsfde rsefvkfrek leslwaaqgm avtapvesym vpcvqyatmp
      181 fmppypvaad avtsppyvqg csrthnmqka rlssievkpe pqckrsvkls lqwsdrhqte
      241 vvrsfqeisg lsqklsrlsa sdptcsgkai pqlpaciekn eeldgmfelv eyfnwvaslp
      301 ssylenelvg pffepapcgk pgfvpppavg petslplape dprvgllafy gasadaplaa
      361 appllaptvg smmppmvaps vncagqgaeh agpspcnspl ssapaspyes pptsgcnsra
      421 tspwtgtavt rmhwdgapss veellhrahl kkyshafknc slddvlamne eamkhkglpa
      481 scsrrllkei allkshtngv lssrtiatga aaafshasge ssttscssec sspspppalk
      541 avrthspass gsddstqpst gssygpnaak wpttgetveg tgsmvtpeta rlgsqapsvm
      601 plryappgsv lmepqprfvl ltdrptygps rapvralapv vktaatdgap hsnpavttta
      661 afapmysvgi mpagmfpqhs fppflhaghq sngylsflsg agyvapptta atyvplatfg
      721 apsvvvpppn sakvascync glvghrghec keatveeatk saqfrlhfap tgaapeaqp
//
LOCUS       XP_077503258             134 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome b5 [Amblyomma americanum].
ACCESSION   XP_077503258
VERSION     XP_077503258.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647132.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..134
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..134
                     /product="cytochrome b5"
                     /calculated_mol_wt=14854
     Region          9..80
                     /region_name="Cyt-b5"
                     /note="Cytochrome b5-like Heme/Steroid binding domain;
                     pfam00173"
                     /db_xref="CDD:459698"
     CDS             1..134
                     /gene="Cyt-b5"
                     /coded_by="XM_077647132.1:143..547"
                     /db_xref="GeneID:144113797"
ORIGIN      
        1 matptktytl aeiekhnekt sawllihnav ydvtkfmeeh pggeevlleq agkhateafe
       61 dvghstdare lmkqykigdl ceddqkkigq vakktqwaat tsnesswmsw lipvgvaaaa
      121 silyrlflsy gahq
//
LOCUS       XP_077503259             329 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113798 [Amblyomma americanum].
ACCESSION   XP_077503259
VERSION     XP_077503259.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647133.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..329
                     /product="uncharacterized protein LOC144113798"
                     /calculated_mol_wt=36683
     CDS             1..329
                     /gene="LOC144113798"
                     /coded_by="XM_077647133.1:104..1093"
                     /db_xref="GeneID:144113798"
ORIGIN      
        1 myrsylcvev villllshvg wngpssprvq pcsraqshva rqlvrrkvta vlqryrvslg
       61 pecplhpsrd lfwwppnesk hswscpmcgr sffcheklts hwdqehapqm ppkadrgvcl
      121 adycdilrcd vlgprvaaae rasasgssav vealpdkpka degrcgsppa adhprcscdq
      181 rhmsalqqkc rivvqqcvig llsvlsvkdf qdiedelnng icsyltcnky wddslneerr
      241 vpmlfsmiig iilvggfclc yyivwilfea iplpslgspc ggaayddvyc snsqvryssf
      301 grdgparqch crvlgrhayq khntaanvr
//
LOCUS       XP_077503260             571 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-4-like [Amblyomma americanum].
ACCESSION   XP_077503260
VERSION     XP_077503260.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647134.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..571
                     /product="protein argonaute-4-like"
                     /calculated_mol_wt=63249
     Region          140..>555
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     CDS             1..571
                     /gene="LOC144113799"
                     /coded_by="XM_077647134.1:69..1784"
                     /db_xref="GeneID:144113799"
ORIGIN      
        1 mhngkkksds adgrpavpsv sggnqatasp pgpsrvsfeq alqqgvaasp qpravsgsqt
       61 paaersaqps vepsgyrpiy gaaaaagaan vaasspwpas aaaavsgcqp aaaapppspp
      121 rtspvvvtqq tpeagdqttp gddvkirele rtlpshfprr pahgqlgrti qllanhfsie
      181 iptgsvyhyd vdissetaqe tkvpeqkkyp clstkinriv iellvkkyrq dlancipafd
      241 grknlytrrq vnfrertftv dleedqrsqk fiikiqyaat vnlealhgvf ekrvqtvphe
      301 vlqaidivlr hspsinlapv grsffrppgp nehndlgggr evwfgyytsv rpaqwmpmln
      361 vdmsatafye slplvdfmcr flsdsrrvlt ptdfrslrdn hyvrlnrelk glrvkvthlp
      421 yprkykvvki trepakdiyf esegsqisva dyfqsryrrl sypnfpcvqr gspthpvyip
      481 levcelaegq hcrkkldesq taemikrtak ppakrfheir qsvrdmvsss dkylrefgvk
      541 inteptqvvg rvldptgwas satflparps s
//
LOCUS       XP_077503262             105 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113803 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503262
VERSION     XP_077503262.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647136.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..105
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..105
                     /product="uncharacterized protein LOC144113803 isoform X1"
                     /calculated_mol_wt=11637
     CDS             1..105
                     /gene="LOC144113803"
                     /coded_by="XM_077647136.1:1237..1554"
                     /db_xref="GeneID:144113803"
ORIGIN      
        1 melgvtkael iqrmskrkit akdfeesepg ttvaqihsst smdpaapqnp gillrlplaw
       61 vllhhgtqvf llrsmarydg fsylelvckl emllalvlgn crrti
//
LOCUS       XP_077503263              79 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113803 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503263
VERSION     XP_077503263.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647137.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..79
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..79
                     /product="uncharacterized protein LOC144113803 isoform X2"
                     /calculated_mol_wt=8637
     CDS             1..79
                     /gene="LOC144113803"
                     /coded_by="XM_077647137.1:1237..1476"
                     /db_xref="GeneID:144113803"
ORIGIN      
        1 melgvtkael iqrmskrkit akdfeesepg ttvaqihsst smdpaapqnp gillrlplaw
       61 vllhhgtqvf llrsmarci
//
LOCUS       XP_077503264             388 aa            linear   INV 25-NOV-2025
DEFINITION  snRNA-activating protein complex subunit 3-like [Amblyomma
            americanum].
ACCESSION   XP_077503264
VERSION     XP_077503264.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647138.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..388
                     /product="snRNA-activating protein complex subunit 3-like"
                     /calculated_mol_wt=44590
     Region          178..381
                     /region_name="SNAPC3"
                     /note="snRNA-activating protein complex (SNAPc), subunit
                     3; pfam12251"
                     /db_xref="CDD:463506"
     CDS             1..388
                     /gene="LOC144113805"
                     /coded_by="XM_077647138.1:140..1306"
                     /db_xref="GeneID:144113805"
ORIGIN      
        1 mdavhepksr gfisepikva efkeqwtnal eawrfyefdd mrigeairka mniseselkr
       61 lekefrpdkl ccglenldrt elpevtdlmt iklqkeelqr qqqdtkyryk aaslvrrscp
      121 ltsvnlvsnn srvkppdter vpsgeailtv qvfkpiktpd yftkvhrgcn sfpfkilsev
      181 avlgsqtlle lrakiqcvsd dvpigdfsen pdkpqgpaas eiyksgfffi gntfyndmsd
      241 pscrdyseai iewakeprrg lgpfkkaimg dvtfdqleic lgypyvykhq gscehllvfs
      301 dirmhhahds qhlldypfvv knfpvgkrvl cnlcrkstak wvttenrrvp ddpyffcgac
      361 frkfnytadn kkigsfralp ykdwnavl
//
LOCUS       XP_077503265             388 aa            linear   INV 25-NOV-2025
DEFINITION  snRNA-activating protein complex subunit 3-like [Amblyomma
            americanum].
ACCESSION   XP_077503265
VERSION     XP_077503265.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647139.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..388
                     /product="snRNA-activating protein complex subunit 3-like"
                     /calculated_mol_wt=44590
     Region          178..381
                     /region_name="SNAPC3"
                     /note="snRNA-activating protein complex (SNAPc), subunit
                     3; pfam12251"
                     /db_xref="CDD:463506"
     CDS             1..388
                     /gene="LOC144113805"
                     /coded_by="XM_077647139.1:88..1254"
                     /db_xref="GeneID:144113805"
ORIGIN      
        1 mdavhepksr gfisepikva efkeqwtnal eawrfyefdd mrigeairka mniseselkr
       61 lekefrpdkl ccglenldrt elpevtdlmt iklqkeelqr qqqdtkyryk aaslvrrscp
      121 ltsvnlvsnn srvkppdter vpsgeailtv qvfkpiktpd yftkvhrgcn sfpfkilsev
      181 avlgsqtlle lrakiqcvsd dvpigdfsen pdkpqgpaas eiyksgfffi gntfyndmsd
      241 pscrdyseai iewakeprrg lgpfkkaimg dvtfdqleic lgypyvykhq gscehllvfs
      301 dirmhhahds qhlldypfvv knfpvgkrvl cnlcrkstak wvttenrrvp ddpyffcgac
      361 frkfnytadn kkigsfralp ykdwnavl
//
LOCUS       XP_077503266             557 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113806 [Amblyomma americanum].
ACCESSION   XP_077503266
VERSION     XP_077503266.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647140.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..557
                     /product="uncharacterized protein LOC144113806"
                     /calculated_mol_wt=59730
     Region          36..153
                     /region_name="CUB"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; cd00041"
                     /db_xref="CDD:238001"
     Site            order(45,47,49,76,81,122,148,150,152..153)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     Region          162..196
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(167,175,186..187)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(179,182,186,192..193)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            189..193
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          198..233
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(203,212,223..224)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(216,219,223,229..230)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            226..230
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          323..352
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(323,331,342..343)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(335,338,342,348..349)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            345..349
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     CDS             1..557
                     /gene="LOC144113806"
                     /coded_by="XM_077647140.1:174..1847"
                     /db_xref="GeneID:144113806"
ORIGIN      
        1 mrqlfsvlll aavvcsgsts vrgllqssgg adigscqqqr rlgprgkiss pgypasysnh
       61 tscswfiigr hdqiitisfd dldlggledc eknpccggtw iklgptpngg ervhcgrkap
      121 epfvsrsskv wikfhtssiq vpqgrgfqls ytvgtykqps scrpdefqcl ngmcifqhwa
      181 cnghtecedk sdellcacpp gmlhcatgsg cynrkarcng qpdcpdlsde ldcafcgpnr
      241 tlcsptsttc ydplsercds ifhcengede qgcvsgceqk imcasgvgcy raqdrcdgvp
      301 hcadrsdetg cgpekcrser gaylcgdgrc vaedtvcdrv tdcpdgsdey gclrnsmita
      361 aiigslfcgl lvvvavscsc rlyalrlaqq qaaaaegpmd spplfvdsap pdfwfreppp
      421 pyavavgdhr yhvpadgviv ygpggtqsdl gtfcgrgtqr rtrrlrrhrr rppspptpsi
      481 tshetgssee alatavhgds slvlsvhtat tsrrkvstts vqsegtsrtp lpvaaadsvv
      541 scddtrplie edprgst
//
LOCUS       XP_077503267             203 aa            linear   INV 25-NOV-2025
DEFINITION  dual specificity protein phosphatase 3-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503267
VERSION     XP_077503267.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647141.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..203
                     /product="dual specificity protein phosphatase 3-like
                     isoform X1"
                     /calculated_mol_wt=22251
     Region          55..201
                     /region_name="DUSP3-like"
                     /note="dual specificity protein phosphatases 3, 13, 26,
                     27, and similar domains; cd14515"
                     /db_xref="CDD:350365"
     CDS             1..203
                     /gene="LOC144113808"
                     /coded_by="XM_077647141.1:402..1013"
                     /db_xref="GeneID:144113808"
ORIGIN      
        1 msflgcdnsf amrglhqilv evkpelkplp gvallakshg lkapptdrys ygvdcdevyp
       61 gifvgdegaa rnkrylrglg vthvlnaaeg pqfgqvdtnq afydshaisy lglrlvdipq
      121 ediaahfdkc adfiedcleh ngkvlvncrm gmsrsatiai aylmikkgmt vddglrtlrm
      181 nravrpnngf llqlvqldak lrs
//
LOCUS       XP_077503268             164 aa            linear   INV 25-NOV-2025
DEFINITION  dual specificity protein phosphatase 3-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503268
VERSION     XP_077503268.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647142.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..164
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..164
                     /product="dual specificity protein phosphatase 3-like
                     isoform X2"
                     /calculated_mol_wt=17935
     Region          55..>141
                     /region_name="PTP_DSP_cys"
                     /note="cys-based protein tyrosine phosphatase and
                     dual-specificity phosphatase superfamily; cl28904"
                     /db_xref="CDD:475123"
     CDS             1..164
                     /gene="LOC144113808"
                     /coded_by="XM_077647142.1:402..896"
                     /db_xref="GeneID:144113808"
ORIGIN      
        1 msflgcdnsf amrglhqilv evkpelkplp gvallakshg lkapptdrys ygvdcdevyp
       61 gifvgdegaa rnkrylrglg vthvlnaaeg pqfgqvdtnq afydshaisy lglrlvdipq
      121 ediaahfdkc adfiedcleh neyaieatnr gvatnrrlsn qqea
//
LOCUS       XP_077503269             421 aa            linear   INV 25-NOV-2025
DEFINITION  pachytene checkpoint 2 protein [Amblyomma americanum].
ACCESSION   XP_077503269
VERSION     XP_077503269.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647143.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..421
                     /product="pachytene checkpoint 2 protein"
                     /calculated_mol_wt=46818
     Region          114..312
                     /region_name="RecA-like_Pch2-like"
                     /note="ATPase domain of Pachytene checkpoint 2 (Pch2) and
                     similar ATPase domains; cd19508"
                     /db_xref="CDD:410916"
     Site            order(127,144..145,148..149,155..158,160..164,208,
                     210..211,217,304)
                     /site_type="other"
                     /note="hexamer interface [polypeptide binding]"
                     /db_xref="CDD:410916"
     Site            order(173,177..179,204,206..207,245..246)
                     /site_type="active"
                     /note="ATP binding site [active]"
                     /db_xref="CDD:410916"
     CDS             1..421
                     /gene="pch2"
                     /coded_by="XM_077647143.1:107..1372"
                     /db_xref="GeneID:144113809"
ORIGIN      
        1 msynkthlkn qlakdslltp gncyvhadal ksrrctlaen viithveglv rqrgyphilt
       61 dfagtplagq vdkvvteydk dnverlddat lvvhvyylsy detgleeled gmaaanhwvl
      121 psvhleglwe nliyespvkn ellsysstal lfadkkvdpn iiswnkvvll hgppgtgkts
      181 lckalaqklt irlrsrfsqg qlleinshsl fskwfsesgk lvmkmfqkil tliddgttlv
      241 fvlidevesl ahcrsaaicg nepsdairvv nalltqidil krypnvfilt tsnisgvidl
      301 afvdradfky hldypsqtci skifmscmee lrrvsviddv ssllesssde kngelkalfr
      361 slcssavgls grqlrklpfi ahsicfadna ltlrtfllal seavhrqwqg iekiiaskgs
      421 l
//
LOCUS       XP_077503271             206 aa            linear   INV 25-NOV-2025
DEFINITION  AFG2-interacting ribosome maturation factor-like [Amblyomma
            americanum].
ACCESSION   XP_077503271
VERSION     XP_077503271.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647145.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..206
                     /product="AFG2-interacting ribosome maturation
                     factor-like"
                     /calculated_mol_wt=23700
     Region          17..171
                     /region_name="CK2S"
                     /note="Casein Kinase 2 substrate; pfam15011"
                     /db_xref="CDD:464443"
     CDS             1..206
                     /gene="LOC144113810"
                     /coded_by="XM_077647145.1:257..877"
                     /db_xref="GeneID:144113810"
ORIGIN      
        1 mdgitglneh lyeirkafkl afcslkeqkq vwekcmdsar pqlaaivtlr eiqvncrtak
       61 lsgselvtkh pdirqrvvrc venelfqekt klesvmkslk ksqnifsgac qqaikvyekl
      121 sqdlsiedic yrgeiypsla dmlewitcie qqfsshihar ellleetnfg ddykvdvfvr
      181 ewkddaalme slndvlatvk fiiemt
//
LOCUS       XP_077503272             304 aa            linear   INV 25-NOV-2025
DEFINITION  transmembrane protein 64 [Amblyomma americanum].
ACCESSION   XP_077503272
VERSION     XP_077503272.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647146.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..304
                     /product="transmembrane protein 64"
                     /calculated_mol_wt=32684
     Region          91..271
                     /region_name="TVP38"
                     /note="Uncharacterized membrane protein YdjX, related to
                     fungal oxalate transporter, TVP38/TMEM64 family [Function
                     unknown]; COG0398"
                     /db_xref="CDD:440167"
     CDS             1..304
                     /gene="LOC144113811"
                     /coded_by="XM_077647146.1:242..1156"
                     /db_xref="GeneID:144113811"
ORIGIN      
        1 mvssahgrll slrgwapdge advppctivt vehrsppptg ekpaydrswl lkaltavavl
       61 aalvvtllwg kqyvreclvw ldsqdretvc lffvlmytlv afpltwgyil lnfacgyhfg
      121 lvlgvavtag aasvgisvah tvmkrfflgf imarllssev vrstlalldt ghafkvviis
      181 rltpipfglq namfavsqms lgryvtasal glvptqvinv ylgttvrsme evldddataa
      241 agyavllaqv vlsvvlmsli vrkarqelre avlssgcssl asldtafcph fpwvpvaphr
      301 ppav
//
LOCUS       XP_077503273             152 aa            linear   INV 25-NOV-2025
DEFINITION  M-phase phosphoprotein 6 [Amblyomma americanum].
ACCESSION   XP_077503273
VERSION     XP_077503273.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647147.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..152
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..152
                     /product="M-phase phosphoprotein 6"
                     /calculated_mol_wt=17553
     Region          9..126
                     /region_name="MPP6"
                     /note="M-phase phosphoprotein 6; pfam10175"
                     /db_xref="CDD:462978"
     CDS             1..152
                     /gene="Mpp6"
                     /coded_by="XM_077647147.1:344..802"
                     /db_xref="GeneID:144113812"
ORIGIN      
        1 mardisktkl srhlldmkfm kkskekaqkd eeeeehrslf nsdamdcikk egsryvikes
       61 yalclglltp rmsfkgrnpe ierlmqqgge teaklanmpg ipdeemakry stlvgtiakk
      121 fdkrkqsths feegankrkv hkrrkfqkps de
//
LOCUS       XP_077503274             348 aa            linear   INV 25-NOV-2025
DEFINITION  V-type proton ATPase subunit VhaAC39-1 [Amblyomma americanum].
ACCESSION   XP_077503274
VERSION     XP_077503274.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647148.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..348
                     /product="V-type proton ATPase subunit VhaAC39-1"
                     /calculated_mol_wt=39711
     Region          13..341
                     /region_name="vATP-synt_AC39"
                     /note="ATP synthase (C/AC39) subunit; pfam01992"
                     /db_xref="CDD:426553"
     CDS             1..348
                     /gene="VhaAC39-1"
                     /coded_by="XM_077647148.1:68..1114"
                     /db_xref="GeneID:144113813"
ORIGIN      
        1 msqllfnmsn gyleglvrgf kggilkqsdy lnlvqcetle dlklhlqstd ygnflaneas
       61 pltvsviddk lreklvvefr hmrnhavepl atfldyitys ymidniilli tgtlhqrpig
      121 elipkchplg sfdqmeaihi astpaelyna vivdtplapf fvdciseqdl demnieiirn
      181 tlykaylenf ykfcesiggt tadvmleila feadrrafii tinsfgtelt kedraklfpk
      241 cghlypdgln alakaddydq vrsiaeyyaq ynvlfggagn npdertledk ffehevmlnv
      301 nafmqqfhsg vfyayvklke qecrnivwia ecvaqrhrsk idnyipif
//
LOCUS       XP_077503275             451 aa            linear   INV 25-NOV-2025
DEFINITION  F-box only protein 22-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503275
VERSION     XP_077503275.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647149.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..451
                     /product="F-box only protein 22-like isoform X1"
                     /calculated_mol_wt=49493
     Region          47..88
                     /region_name="F-box_SF"
                     /note="F-box domain superfamily; cl45894"
                     /db_xref="CDD:459239"
     Site            order(50..54,57..58,61,72..74,76,79)
                     /site_type="other"
                     /note="F-box motif"
                     /db_xref="CDD:438852"
     Site            order(50,53,56..57,60..61,65,67..68,72..74,76)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438852"
     Region          <373..445
                     /region_name="FIST"
                     /note="Small ligand-binding sensory domain FIST [Signal
                     transduction mechanisms]; COG4398"
                     /db_xref="CDD:443524"
     CDS             1..451
                     /gene="LOC144113814"
                     /coded_by="XM_077647149.1:402..1757"
                     /db_xref="GeneID:144113814"
ORIGIN      
        1 msakraaran artrqaasva aaararkssa mdseekephy svfghvlshs pelselilsy
       61 mscrqlcvca qvctlwnsta krlkskrsnf lhcfetlnrk rkacthgeps rdawgdnsks
      121 flqclrdaws eprhalvfgt hhmyryldap ekaakccsyr aenwnnaaer lashlppscy
      181 lllgaaggvv gmsgsgypve lergegisgl llpaqlpegv rvrpfmvyrh hlrfenypsd
      241 wfasdnfksl vglpvgdetl dvkcvlwfav ndlvfdddeh fmyevvddfv ascgarrfal
      301 ggavldnmtv kdgqkgides aiiggfcfsg dgvrsvsiil qptvrgtrav ereltrlkkt
      361 sgfdtwdkcs tvgfmfacva rgsklhgksn ieadafarvf pgiplmgifg kgeigvncvp
      421 snepvaaktl dftclhgytt vfvllclgkc k
//
LOCUS       XP_077503276             360 aa            linear   INV 25-NOV-2025
DEFINITION  F-box only protein 22-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503276
VERSION     XP_077503276.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647150.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..360
                     /product="F-box only protein 22-like isoform X2"
                     /calculated_mol_wt=39382
     Region          <282..354
                     /region_name="FIST"
                     /note="Small ligand-binding sensory domain FIST [Signal
                     transduction mechanisms]; COG4398"
                     /db_xref="CDD:443524"
     CDS             1..360
                     /gene="LOC144113814"
                     /coded_by="XM_077647150.1:16..1098"
                     /db_xref="GeneID:144113814"
ORIGIN      
        1 mlvctlnrkr kacthgepsr dawgdnsksf lqclrdawse prhalvfgth hmyryldape
       61 kaakccsyra enwnnaaerl ashlppscyl llgaaggvvg msgsgypvel ergegisgll
      121 lpaqlpegvr vrpfmvyrhh lrfenypsdw fasdnfkslv glpvgdetld vkcvlwfavn
      181 dlvfdddehf myevvddfva scgarrfalg gavldnmtvk dgqkgidesa iiggfcfsgd
      241 gvrsvsiilq ptvrgtrave reltrlkkts gfdtwdkcst vgfmfacvar gsklhgksni
      301 eadafarvfp giplmgifgk geigvncvps nepvaaktld ftclhgyttv fvllclgkck
//
LOCUS       XP_077503277             167 aa            linear   INV 25-NOV-2025
DEFINITION  glycine cleavage system H protein, mitochondrial [Amblyomma
            americanum].
ACCESSION   XP_077503277
VERSION     XP_077503277.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647151.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..167
                     /product="glycine cleavage system H protein,
                     mitochondrial"
                     /calculated_mol_wt=18257
     Region          44..165
                     /region_name="GcvH"
                     /note="Glycine cleavage system protein H (lipoate-binding)
                     [Amino acid transport and metabolism]; COG0509"
                     /db_xref="CDD:440275"
     CDS             1..167
                     /gene="ppl"
                     /coded_by="XM_077647151.1:132..635"
                     /db_xref="GeneID:144113815"
ORIGIN      
        1 maavrcivqs aaqhsfylkr gafkslqrya gssrcftlsa sskaerrfse khewvvvdgd
       61 vgtvgisqha qealgdvvyv qppdigtelt qhdecgaies vkaaseiytp vsgkvtevnk
      121 aledkpslvn sscydegwif klkvkntdel kslmdekaye eflkssa
//
LOCUS       XP_077503278              97 aa            linear   INV 25-NOV-2025
DEFINITION  dynein light chain roadblock-type 2-like [Amblyomma americanum].
ACCESSION   XP_077503278
VERSION     XP_077503278.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647152.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..97
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..97
                     /product="dynein light chain roadblock-type 2-like"
                     /calculated_mol_wt=10739
     Region          <26..92
                     /region_name="Robl_LC7"
                     /note="Roadblock/LC7 domain; smart00960"
                     /db_xref="CDD:214939"
     CDS             1..97
                     /gene="LOC144113816"
                     /coded_by="XM_077647152.1:234..527"
                     /db_xref="GeneID:144113816"
ORIGIN      
        1 mtseveeifk klkdqegvvg vvvttaegap irtsfdhttt lqysslvtrl ceqarstlrd
       61 lepgndltfl rmrtkkhevm iapdknyclv vvqnpsg
//
LOCUS       XP_077503279              97 aa            linear   INV 25-NOV-2025
DEFINITION  dynein light chain roadblock-type 2-like [Amblyomma americanum].
ACCESSION   XP_077503279
VERSION     XP_077503279.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647153.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..97
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..97
                     /product="dynein light chain roadblock-type 2-like"
                     /calculated_mol_wt=10739
     Region          <26..92
                     /region_name="Robl_LC7"
                     /note="Roadblock/LC7 domain; smart00960"
                     /db_xref="CDD:214939"
     CDS             1..97
                     /gene="LOC144113816"
                     /coded_by="XM_077647153.1:217..510"
                     /db_xref="GeneID:144113816"
ORIGIN      
        1 mtseveeifk klkdqegvvg vvvttaegap irtsfdhttt lqysslvtrl ceqarstlrd
       61 lepgndltfl rmrtkkhevm iapdknyclv vvqnpsg
//
LOCUS       XP_077503280             285 aa            linear   INV 25-NOV-2025
DEFINITION  lens fiber major intrinsic protein-like [Amblyomma americanum].
ACCESSION   XP_077503280
VERSION     XP_077503280.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647154.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..285
                     /product="lens fiber major intrinsic protein-like"
                     /calculated_mol_wt=30057
     Region          29..236
                     /region_name="MIP"
                     /note="Major intrinsic protein (MIP) superfamily. Members
                     of the MIP superfamily function as membrane channels that
                     selectively transport water, small neutral molecules, and
                     ions out of and between cells. The channel proteins share
                     a common fold: the N-terminal...; cl00200"
                     /db_xref="CDD:444743"
     Site            order(90..92,197..198,201,204)
                     /site_type="active"
                     /note="amphipathic channel [active]"
                     /db_xref="CDD:238204"
     Site            order(92..94,201..203)
                     /site_type="other"
                     /note="Asn-Pro-Ala signature motifs"
                     /db_xref="CDD:238204"
     CDS             1..285
                     /gene="LOC144113818"
                     /coded_by="XM_077647154.1:9..866"
                     /db_xref="GeneID:144113818"
ORIGIN      
        1 masapcsplr rmrrlmdslp eepppcrrel asgplwrrvf vellgsllft vftvplqprr
       61 ggqpvdsapl qcsvamgasa aalahclagd lnpavtlsrl vgrrlsvvra llcvaaqcsg
      121 aclgaaalyg laprdqrpaq qppqqlaqgl hasqafgvef lgtvlvalsa laadraasgp
      181 lgvayaaaal fayrftgagl nparslgpal itntwshhwv ywagpmlggl magftheytr
      241 garvsrehsl rpsppadqpa rnhvkrdisg lsndteltsn ndcrv
//
LOCUS       XP_077503281             467 aa            linear   INV 25-NOV-2025
DEFINITION  carboxypeptidase Q-like [Amblyomma americanum].
ACCESSION   XP_077503281
VERSION     XP_077503281.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647155.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..467
                     /product="carboxypeptidase Q-like"
                     /calculated_mol_wt=51297
     Region          39..462
                     /region_name="Zinc_peptidase_like"
                     /note="Zinc peptidases M18, M20, M28, and M42; cl14876"
                     /db_xref="CDD:472712"
     CDS             1..467
                     /gene="LOC144113819"
                     /coded_by="XM_077647155.1:127..1530"
                     /db_xref="GeneID:144113819"
ORIGIN      
        1 marsflpllv allaaaarrt havcivpdep freirnaseq vqnlitlfrs gaekgttyre
       61 lsqfadrfgs rmlgsvqlek sidnllerfk ldhfqdvhte dvlavrwrrq heearlvrpe
      121 ehplrvlglg gssgtppggv tapldvvksf aelemkgasv sghivlympe wkghgsyasy
      181 llkgtskaar lgalgalfra pepltgtalf agltgyekda priaaavltk qdadllarmv
      241 drrqevvvhm rmdpgepqqv vsrnilvewk gskypkeyvl vgahidswdi gqgaadnaaa
      301 vfivwralsv lkrlglqpkr tvrfvvwtar eqgmegatef frryaadsgn ttmaleldla
      361 afaptglals nvrnndtvci vremlkefea idangltrgg gapelrlwre agvpvagfnt
      421 awyfhddmrh seadtvsgiq sgdldlsasm vaglvhmlgq merklpr
//
LOCUS       XP_077503283             474 aa            linear   INV 25-NOV-2025
DEFINITION  carboxypeptidase Q-like [Amblyomma americanum].
ACCESSION   XP_077503283
VERSION     XP_077503283.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647157.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..474
                     /product="carboxypeptidase Q-like"
                     /calculated_mol_wt=52102
     Region          42..468
                     /region_name="Zinc_peptidase_like"
                     /note="Zinc peptidases M18, M20, M28, and M42; cl14876"
                     /db_xref="CDD:472712"
     CDS             1..474
                     /gene="LOC144113820"
                     /coded_by="XM_077647157.1:51..1475"
                     /db_xref="GeneID:144113820"
ORIGIN      
        1 mgrlvvllpl lllvaawasg devytcngtq llsaellaei rsyapvvqsi vdyviqgper
       61 nrtyeelakf vdrfgarisg sqnlensidy mvellgrqgl dsvytervav phwvrgnesa
      121 wivrprmqrl nmlglggsvg tppggiiapv lvvrsfqelh snvskargkv vvfdedfssy
      181 artvlyreng aaeaervgav aalvrsvtpf sigsphtgwm nypngeprip caaitvedaq
      241 llrrlqnrgi evyvhlimda knlplaasrn tiaelrgttk seelvlvsgh ldswdvgqga
      301 mddgggafis wralallrrl glrprrtlra llwtgeeegv wggrayyerh gvhdrgrlsl
      361 vmesdlgtfr plglrlaaad prarcmaaqv mrlfeainat rltlgadgpd iqpwvdagvp
      421 gasldtenke yfyfhhtdgd smtvedptnl dlctafwaat ayvfadlser lprp
//
LOCUS       XP_077503284             265 aa            linear   INV 25-NOV-2025
DEFINITION  PSME3-interacting protein [Amblyomma americanum].
ACCESSION   XP_077503284
VERSION     XP_077503284.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647158.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..265
                     /product="PSME3-interacting protein"
                     /calculated_mol_wt=29859
     Region          18..119
                     /region_name="FAM192A_Fyv6_N"
                     /note="FAM192A/Fyv6, N-terminal domain; pfam10187"
                     /db_xref="CDD:462987"
     CDS             1..265
                     /gene="LOC144113821"
                     /coded_by="XM_077647158.1:238..1035"
                     /db_xref="GeneID:144113821"
ORIGIN      
        1 msfggstsqe tipkfksfvs eneleerrkk rqeewekvrk qddpveapee eydprslfer
       61 leeqrlkkqa dyeeahklkn mirgldddev tfleyvdmrk qeivsqrmke dmqeieeyrk
      121 avatlseeal eakrlelkks tgavpasgqg kksqlsllsg avkrkcgdtp sdaekkqctn
      181 ssaeqqddtv cegdeersqs pgevrvqapv talhclgvlp glgvytdssd senssfsdea
      241 dleldlvgrr rpaamhqaer spkpn
//
LOCUS       XP_077503285             145 aa            linear   INV 25-NOV-2025
DEFINITION  NADH dehydrogenase (ubiquinone) B22 subunit [Amblyomma americanum].
ACCESSION   XP_077503285
VERSION     XP_077503285.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647159.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..145
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..145
                     /product="NADH dehydrogenase (ubiquinone) B22 subunit"
                     /calculated_mol_wt=17608
     Region          11..87
                     /region_name="Complex1_LYR_NDUFB9_LYRM3"
                     /note="LYR (leucine-tyrosine-arginine) motif found in NADH
                     dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
                     (NDUFB9) and similar proteins; cd20263"
                     /db_xref="CDD:380758"
     Site            order(11..12,15,22,25,46..47,50..51,59,62,66,70,73)
                     /site_type="other"
                     /note="phosphopantetheine binding site [chemical binding]"
                     /db_xref="CDD:380758"
     Site            order(13,16..17,19..21,23..24,28..29,33..35,37..39,41,
                     44..45,64,67..69,71..79,81..85,87)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:380758"
     CDS             1..145
                     /gene="ND-B22"
                     /coded_by="XM_077647159.1:98..535"
                     /db_xref="GeneID:144113823"
ORIGIN      
        1 maylvtrtvs hkfqvcslyk ralraleawn lerddyrisa vqlrqrfemy rhikdlrvak
       61 elldegeeel fqkehpiplh yplspqgvay greeitpdwv ldfwhpleka qypeyfarre
      121 qrkkeyienw ekqygkpqpe aehhh
//
LOCUS       XP_077503286             254 aa            linear   INV 25-NOV-2025
DEFINITION  type 2 phosphatidylinositol 4,5-bisphosphate 4-phosphatase-like
            [Amblyomma americanum].
ACCESSION   XP_077503286
VERSION     XP_077503286.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647160.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..254
                     /product="type 2 phosphatidylinositol 4,5-bisphosphate
                     4-phosphatase-like"
                     /calculated_mol_wt=27661
     Region          <48..249
                     /region_name="Tmemb_55A"
                     /note="Transmembrane protein 55A; pfam09788"
                     /db_xref="CDD:462901"
     CDS             1..254
                     /gene="LOC144113824"
                     /coded_by="XM_077647160.1:138..902"
                     /db_xref="GeneID:144113824"
ORIGIN      
        1 mayaesspll aqstsgnyss ayptapsggg spppsyedvg ympsrtkacd vcnysidisn
       61 hgncyvvpcp rcnevtalgn ppkgkkyirc tcnkllqcvk satrvkcdrp gcgaelqaed
      121 iavtkahakq prdaakrgrr eeemaephre htyrficahc esvsvldtap sssfrcrhcg
      181 krssvgqsnk kaiclitagt vvvmlvivlm astltlkhns gywwlylvgl ivavffhgyg
      241 lwllcmkrst vevr
//
LOCUS       XP_077503287             254 aa            linear   INV 25-NOV-2025
DEFINITION  type 2 phosphatidylinositol 4,5-bisphosphate 4-phosphatase-like
            [Amblyomma americanum].
ACCESSION   XP_077503287
VERSION     XP_077503287.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647161.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..254
                     /product="type 2 phosphatidylinositol 4,5-bisphosphate
                     4-phosphatase-like"
                     /calculated_mol_wt=27661
     Region          <48..249
                     /region_name="Tmemb_55A"
                     /note="Transmembrane protein 55A; pfam09788"
                     /db_xref="CDD:462901"
     CDS             1..254
                     /gene="LOC144113824"
                     /coded_by="XM_077647161.1:125..889"
                     /db_xref="GeneID:144113824"
ORIGIN      
        1 mayaesspll aqstsgnyss ayptapsggg spppsyedvg ympsrtkacd vcnysidisn
       61 hgncyvvpcp rcnevtalgn ppkgkkyirc tcnkllqcvk satrvkcdrp gcgaelqaed
      121 iavtkahakq prdaakrgrr eeemaephre htyrficahc esvsvldtap sssfrcrhcg
      181 krssvgqsnk kaiclitagt vvvmlvivlm astltlkhns gywwlylvgl ivavffhgyg
      241 lwllcmkrst vevr
//
LOCUS       XP_077503288             254 aa            linear   INV 25-NOV-2025
DEFINITION  type 2 phosphatidylinositol 4,5-bisphosphate 4-phosphatase-like
            [Amblyomma americanum].
ACCESSION   XP_077503288
VERSION     XP_077503288.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647162.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..254
                     /product="type 2 phosphatidylinositol 4,5-bisphosphate
                     4-phosphatase-like"
                     /calculated_mol_wt=27661
     Region          <48..249
                     /region_name="Tmemb_55A"
                     /note="Transmembrane protein 55A; pfam09788"
                     /db_xref="CDD:462901"
     CDS             1..254
                     /gene="LOC144113824"
                     /coded_by="XM_077647162.1:79..843"
                     /db_xref="GeneID:144113824"
ORIGIN      
        1 mayaesspll aqstsgnyss ayptapsggg spppsyedvg ympsrtkacd vcnysidisn
       61 hgncyvvpcp rcnevtalgn ppkgkkyirc tcnkllqcvk satrvkcdrp gcgaelqaed
      121 iavtkahakq prdaakrgrr eeemaephre htyrficahc esvsvldtap sssfrcrhcg
      181 krssvgqsnk kaiclitagt vvvmlvivlm astltlkhns gywwlylvgl ivavffhgyg
      241 lwllcmkrst vevr
//
LOCUS       XP_077503289             254 aa            linear   INV 25-NOV-2025
DEFINITION  type 2 phosphatidylinositol 4,5-bisphosphate 4-phosphatase-like
            [Amblyomma americanum].
ACCESSION   XP_077503289
VERSION     XP_077503289.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647163.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..254
                     /product="type 2 phosphatidylinositol 4,5-bisphosphate
                     4-phosphatase-like"
                     /calculated_mol_wt=27661
     Region          <48..249
                     /region_name="Tmemb_55A"
                     /note="Transmembrane protein 55A; pfam09788"
                     /db_xref="CDD:462901"
     CDS             1..254
                     /gene="LOC144113824"
                     /coded_by="XM_077647163.1:142..906"
                     /db_xref="GeneID:144113824"
ORIGIN      
        1 mayaesspll aqstsgnyss ayptapsggg spppsyedvg ympsrtkacd vcnysidisn
       61 hgncyvvpcp rcnevtalgn ppkgkkyirc tcnkllqcvk satrvkcdrp gcgaelqaed
      121 iavtkahakq prdaakrgrr eeemaephre htyrficahc esvsvldtap sssfrcrhcg
      181 krssvgqsnk kaiclitagt vvvmlvivlm astltlkhns gywwlylvgl ivavffhgyg
      241 lwllcmkrst vevr
//
LOCUS       XP_077503290             248 aa            linear   INV 25-NOV-2025
DEFINITION  proteasome alpha4 subunit [Amblyomma americanum].
ACCESSION   XP_077503290
VERSION     XP_077503290.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647164.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..248
                     /product="proteasome alpha4 subunit"
                     /calculated_mol_wt=27885
     Region          6..212
                     /region_name="proteasome_alpha_type_7"
                     /note="The 20S proteasome, multisubunit proteolytic
                     complex, is the central enzyme of nonlysosomal protein
                     degradation in both the cytosol and nucleus. It is
                     composed of 28 subunits arranged as four homoheptameric
                     rings that stack on top of one another forming...;
                     cd03755"
                     /db_xref="CDD:239724"
     Site            order(8..11,13..14,16..17,21,24,27..28,31,39,54,56..57,
                     80..82,84..85,116,119,122..123,126..130,148,153..154,156,
                     158..159,161)
                     /site_type="other"
                     /note="alpha subunit interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:239724"
     Site            order(33,49,51,64,166)
                     /site_type="active"
                     /db_xref="CDD:239724"
     CDS             1..248
                     /gene="Prosalpha4"
                     /coded_by="XM_077647164.1:124..870"
                     /db_xref="GeneID:144113826"
ORIGIN      
        1 mgtarydrai tvfspdghlf qveyaqeavk kgstavgikg tnivvlavek ksvaklqeer
       61 tvrkiclldd hvvmafaglt adarilidra rvecqshklt vedpvtleyi tryiaqlkqr
      121 ytqsngrrpf gisaliggfd fdgtprlfqt dpsgvyhewk anatgrnakt vreyleknys
      181 adkietdega vklairalle viqghnleva imergqplkm leskeiekyv eeiekekede
      241 aekkkqkk
//
LOCUS       XP_077503291             131 aa            linear   INV 25-NOV-2025
DEFINITION  putative nuclear transport factor 2 [Amblyomma americanum].
ACCESSION   XP_077503291
VERSION     XP_077503291.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647165.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..131
                     /product="putative nuclear transport factor 2"
                     /calculated_mol_wt=14845
     Region          7..126
                     /region_name="NTF2"
                     /note="Nuclear transport factor 2 (NTF2) domain plays an
                     important role in the trafficking of macromolecules, ions
                     and small molecules between the cytoplasm and nucleus.
                     This bi-directional transport of macromolecules across the
                     nuclear envelope requires many...; cd00780"
                     /db_xref="CDD:238403"
     Site            order(37,44,51,76,78..79,88,110,120..122,124,126)
                     /site_type="active"
                     /note="TAP/p15 interaction [active]"
                     /db_xref="CDD:238403"
     Site            order(42,44..45,73..74,76,78..79,86,90..92,96,98..101,104,
                     110,120..122,124..126)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:238403"
     Site            order(45..46,63,68,91,93,95..96,98,101,123,125)
                     /site_type="active"
                     /note="RanGDP-NTF2 interaction [active]"
                     /db_xref="CDD:238403"
     CDS             1..131
                     /gene="LOC144113827"
                     /coded_by="XM_077647165.1:140..535"
                     /db_xref="GeneID:144113827"
ORIGIN      
        1 msllnaqydt igrtfiqqyy amfddpalrp nlgtlyneek slmtfegqqi fgrtkileki
       61 qglgfqkich svtiidcqpm fdggilifvl gqlktdddpa htfnqvfvlk pigesfyveh
      121 dvfrlalhht a
//
LOCUS       XP_077503292             359 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113828 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503292
VERSION     XP_077503292.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647166.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..359
                     /product="uncharacterized protein LOC144113828 isoform X1"
                     /calculated_mol_wt=38289
     Region          195..346
                     /region_name="UBCc_UBE2Q"
                     /note="Ubiquitin-conjugating enzyme E2, catalytic (UBCc)
                     domain of ubiquitin-conjugating enzyme E2 Q1, Q2, and
                     related proteins; cd23802"
                     /db_xref="CDD:467422"
     CDS             1..359
                     /gene="LOC144113828"
                     /coded_by="XM_077647166.1:65..1144"
                     /db_xref="GeneID:144113828"
ORIGIN      
        1 mvntdappaa daaetaagep avaagatrde dapevpvaqg dsseevvadg aqgdaavtah
       61 rskrwsvrgr dkqkgrerga pkppaamaaa alrklfrger hrlstganhn epkkkkesae
      121 gnsssssrll slcagkasse raqgkaaaar kpsadgassg spdeaaaaaa pvsrparspr
      181 hrvapcsqqa avrarrlmre lheirrqqar sqppsftvdl vednlyewhv rlyqvdpesp
      241 lwrdmqeagv scvqlsvsfp enfpfappfv rvlsphierg fvmeggaicm elltprgwas
      301 aytveailvq vaaslskgqa riatkpgkqf nrklaessfr slvkthdkyg wvtppladg
//
LOCUS       XP_077503293             273 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin-conjugating enzyme E2Q-like protein 1 isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077503293
VERSION     XP_077503293.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647167.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..273
                     /product="ubiquitin-conjugating enzyme E2Q-like protein 1
                     isoform X2"
                     /calculated_mol_wt=29634
     Region          109..260
                     /region_name="UBCc_UBE2Q"
                     /note="Ubiquitin-conjugating enzyme E2, catalytic (UBCc)
                     domain of ubiquitin-conjugating enzyme E2 Q1, Q2, and
                     related proteins; cd23802"
                     /db_xref="CDD:467422"
     CDS             1..273
                     /gene="LOC144113828"
                     /coded_by="XM_077647167.1:372..1193"
                     /db_xref="GeneID:144113828"
ORIGIN      
        1 maaaalrklf rgerhrlstg anhnepkkkk esaegnssss srllslcagk asseraqgka
       61 aaarkpsadg assgspdeaa aaaapvsrpa rsprhrvapc sqqaavrarr lmrelheirr
      121 qqarsqppsf tvdlvednly ewhvrlyqvd pesplwrdmq eagvscvqls vsfpenfpfa
      181 ppfvrvlsph iergfvmegg aicmelltpr gwasaytvea ilvqvaasls kgqariatkp
      241 gkqfnrklae ssfrslvkth dkygwvtppl adg
//
LOCUS       XP_077503295            1582 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: CD109 antigen-like [Amblyomma americanum].
ACCESSION   XP_077503295
VERSION     XP_077503295.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647169.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1582
                     /product="LOW QUALITY PROTEIN: CD109 antigen-like"
                     /calculated_mol_wt=178742
     Region          <132..1520
                     /region_name="YfaS"
                     /note="Uncharacterized conserved protein YfaS,
                     alpha-2-macroglobulin family [General function prediction
                     only]; COG2373"
                     /db_xref="CDD:441940"
     Region          706..734
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(717,720,724,730..731)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            727..731
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          981..1316
                     /region_name="TED_complement"
                     /note="A-macroglobulin TED domain; pfam07678"
                     /db_xref="CDD:462227"
     CDS             1..1582
                     /gene="LOC144113829"
                     /coded_by="XM_077647169.1:29..4777"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:144113829"
ORIGIN      
        1 mlaaavllva llslssaqya gytdnridsv pihhqqpthl ivasrlvrpg qtfrvvvtll
       61 lqmpepvtvl asiqrngvev tqnsrecrap ipeeillkip ptsvpgsyrl rvegningvl
      121 ggtafsyeta lefsqrsmti fiqtdrplyk qgqtvhfral pvttelkafs davdvymltp
      181 nrtvvrrwls rrtnlgavsl eyplspqpdy gkwtiqviaq gqveeksfvv eeyyqtrfev
      241 nvtlptffma tqeyiygtvv anmtsgaavi gnltvratve pinptyqihr sssryiekvy
      301 ryfegvrdfa fslrelgrlv srlegmkvvv talvgerfld lietgfsesi vfnsslqlrf
      361 lgsspqvfrp gmpfkayvsv sysdgsplpa eyfrnkrvee mlrvtpsvtf vsggagggql
      421 sepvatavpg ssgiwelmle stpalsdpta vnavrairle aefydstygh vrtslvayss
      481 ysptnhhlqi ttstkfpkvg eyiifhvrsn yyvewfsyli iakgiillsg qekmtssikt
      541 favtlspema ptatvviydl argsevvaes ltfpvdgisi dkfnvslnnr kdktgdtiev
      601 aiygdpgtyv alsavdltly rmqagteisy sevlrkmnsf dtiingtltq ewfsrdgniy
      661 syacfpssty gldsnrtfef tgllvfsdsn vtrmrddcnl tagygtcydg acyrlerqcd
      721 gyfdcedgsd etrcedeqrf siprfemyrt nryqrlytns wlwkdinigp lgyyifnipv
      781 pdvptewmis afgmdessgf gilsnsisfs sakpfymnve mptkirlgeq lgirvtvfny
      841 rlyetevlit lasspdyrfv lvgplgrvns yapdtssgeh qhlifikpgk savvympiva
      901 vrtgtievtv laktqiakdm vtrsllvepd gipqyrhtsm vldltqgayl ikyldtnite
      961 spilpfrqdr lyvfgsnkat lsvvgdvfgp afptmpvnss sllskpfycg eqnmfsfgan
     1021 lftvhylrqt nqrdmhlerq afkylnlgyq rqltyqnedg sfqvfrwhsq psvwltafca
     1081 rvfhkatfqe weqflyidpt viqkaiswll drqspegsfh etsfyaydrk mslpserped
     1141 pvrfrnvslt ahvlitlaev rdirgeigsr aataraaaar ylermlhliq kledpyelai
     1201 vayaltlvns adgevafnll dekmretsgm rywsrtdlpa ptvviennkp ylhprlpyvy
     1261 dasnvettay gllvhvkrqa vvqkeivewl ntqrlsyggw astqdtllam eallefsits
     1321 rsrdvtdikv tveapstpgf trelhigpen lsklqtlsip nawgviivka qgtglaivql
     1381 hmeynvdtwk hlvtpppvpa falnirqysy grnsshvsfr scqswirtde sprsgmavle
     1441 vnlpsgyyiq qqtldayvqs gavrnlrear yeekkaeiyf dyldtspicv nftaqrwypv
     1501 anmtrfisir vydyyaperf nesmfevynl yalsichvcg syqcpfcpvf ntapsflhln
     1561 qrqallpltv llvllwrhlf kg
//
LOCUS       XP_077503296             722 aa            linear   INV 25-NOV-2025
DEFINITION  sodium-dependent noradrenaline transporter-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077503296
VERSION     XP_077503296.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647170.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..722
                     /product="sodium-dependent noradrenaline transporter-like
                     isoform X1"
                     /calculated_mol_wt=79748
     Region          159..698
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     Site            order(174,177,515,518..519)
                     /site_type="other"
                     /note="Na binding site [ion binding]"
                     /db_xref="CDD:271356"
     CDS             1..722
                     /gene="LOC144113830"
                     /coded_by="XM_077647170.1:46..2214"
                     /db_xref="GeneID:144113830"
ORIGIN      
        1 mharrgapsq sgcvgaglqa rrgpaqrrar tapseqampn nnsvaaakpp qpaaepllpa
       61 deaaqdaspr sqppqhrspg prsskdspvf feknkvrrmr hsairddgyc sststplsse
      121 yvdhlsgteg qtaviladgv tstvpvaskp hetkvpgerp twknkadfll siigfavdla
      181 nvwrfpylcy rngggvflip ymlmlifgal plfymelvlg qynrqgpisv wklcplfkgv
      241 gycsvlvswy vsfyynviig walyfmfssf raelpwsrcg npwntascys gtlaqndsea
      301 paadnrtspa leffnravle lhtspgmhdl gspkwqlllc vflvfiilyl alfkgvsssg
      361 kvvwvtatap yfiltllllr gvllpgagqg vqyylqpnvg klldtqvwvd aavqvfysvg
      421 vgfgvhltya synpfnnncy rdclmttavn sftsfysgfv ifvylgymaa kqgvpiqqva
      481 teghglvfqv ypeaiatlpg gplwavlffv mlltlgldsa mgglesvitg lmdefktffs
      541 rwryrrevft aivicasfcv sivnvtrggg ymitwldtys agisllcaal fesigiawiy
      601 gldrfcgnih emlgfrpgif wrlcwkfitp tflvaviisg vvnqapveyh nyryppwais
      661 mgwglalssv smvplvaiim lcrakgnckq riaytitpaa erqeekekgv verfklshwt
      721 yv
//
LOCUS       XP_077503297             685 aa            linear   INV 25-NOV-2025
DEFINITION  sodium-dependent noradrenaline transporter-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077503297
VERSION     XP_077503297.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647171.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..685
                     /product="sodium-dependent noradrenaline transporter-like
                     isoform X2"
                     /calculated_mol_wt=75922
     Region          122..661
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     Site            order(137,140,478,481..482)
                     /site_type="other"
                     /note="Na binding site [ion binding]"
                     /db_xref="CDD:271356"
     CDS             1..685
                     /gene="LOC144113830"
                     /coded_by="XM_077647171.1:959..3016"
                     /db_xref="GeneID:144113830"
ORIGIN      
        1 mpnnnsvaaa kppqpaaepl lpadeaaqda sprsqppqhr spgprsskds pvffeknkvr
       61 rmrhsairdd gycsststpl sseyvdhlsg tegqtavila dgvtstvpva skphetkvpg
      121 erptwknkad fllsiigfav dlanvwrfpy lcyrngggvf lipymlmlif galplfymel
      181 vlgqynrqgp isvwklcplf kgvgycsvlv swyvsfyynv iigwalyfmf ssfraelpws
      241 rcgnpwntas cysgtlaqnd seapaadnrt spaleffnra vlelhtspgm hdlgspkwql
      301 llcvflvfii lylalfkgvs ssgkvvwvta tapyfiltll llrgvllpga gqgvqyylqp
      361 nvgklldtqv wvdaavqvfy svgvgfgvhl tyasynpfnn ncyrdclmtt avnsftsfys
      421 gfvifvylgy maakqgvpiq qvateghglv fqvypeaiat lpggplwavl ffvmlltlgl
      481 dsamgglesv itglmdefkt ffsrwryrre vftaivicas fcvsivnvtr gggymitwld
      541 tysagisllc aalfesigia wiygldrfcg nihemlgfrp gifwrlcwkf itptflvavi
      601 isgvvnqapv eyhnyryppw aismgwglal ssvsmvplva iimlcrakgn ckqriaytit
      661 paaerqeeke kgvverfkls hwtyv
//
LOCUS       XP_077503298             685 aa            linear   INV 25-NOV-2025
DEFINITION  sodium-dependent noradrenaline transporter-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077503298
VERSION     XP_077503298.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647172.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..685
                     /product="sodium-dependent noradrenaline transporter-like
                     isoform X2"
                     /calculated_mol_wt=75922
     Region          122..661
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     Site            order(137,140,478,481..482)
                     /site_type="other"
                     /note="Na binding site [ion binding]"
                     /db_xref="CDD:271356"
     CDS             1..685
                     /gene="LOC144113830"
                     /coded_by="XM_077647172.1:177..2234"
                     /db_xref="GeneID:144113830"
ORIGIN      
        1 mpnnnsvaaa kppqpaaepl lpadeaaqda sprsqppqhr spgprsskds pvffeknkvr
       61 rmrhsairdd gycsststpl sseyvdhlsg tegqtavila dgvtstvpva skphetkvpg
      121 erptwknkad fllsiigfav dlanvwrfpy lcyrngggvf lipymlmlif galplfymel
      181 vlgqynrqgp isvwklcplf kgvgycsvlv swyvsfyynv iigwalyfmf ssfraelpws
      241 rcgnpwntas cysgtlaqnd seapaadnrt spaleffnra vlelhtspgm hdlgspkwql
      301 llcvflvfii lylalfkgvs ssgkvvwvta tapyfiltll llrgvllpga gqgvqyylqp
      361 nvgklldtqv wvdaavqvfy svgvgfgvhl tyasynpfnn ncyrdclmtt avnsftsfys
      421 gfvifvylgy maakqgvpiq qvateghglv fqvypeaiat lpggplwavl ffvmlltlgl
      481 dsamgglesv itglmdefkt ffsrwryrre vftaivicas fcvsivnvtr gggymitwld
      541 tysagisllc aalfesigia wiygldrfcg nihemlgfrp gifwrlcwkf itptflvavi
      601 isgvvnqapv eyhnyryppw aismgwglal ssvsmvplva iimlcrakgn ckqriaytit
      661 paaerqeeke kgvverfkls hwtyv
//
LOCUS       XP_077503299             343 aa            linear   INV 25-NOV-2025
DEFINITION  guanidinobutyrase-like [Amblyomma americanum].
ACCESSION   XP_077503299
VERSION     XP_077503299.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647173.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..343
                     /product="guanidinobutyrase-like"
                     /calculated_mol_wt=37027
     Region          44..333
                     /region_name="Agmatinase_PAH"
                     /note="Agmatinase-like family includes proclavaminic acid
                     amidinohydrolase; cd11592"
                     /db_xref="CDD:212538"
     Site            order(68,71,80..81,84,87,112,115..116,276,280,284,
                     288..289,320,323..324,327,331)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:212538"
     Site            order(151,174,176..178,190..191,266,268,310)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:212538"
     CDS             1..343
                     /gene="LOC144113831"
                     /coded_by="XM_077647173.1:164..1195"
                     /db_xref="GeneID:144113831"
ORIGIN      
        1 mqhparwapl lgrrlavqlr hlsrrqgssl nqplsgndcp rpggiatfmr lpyaasahgl
       61 dvavygvpfd cgtsnrsgar fgprqvrces tllrphnmat rtapydslqv advgdvpvtm
      121 ydtqravkdi ehfvrgllkh gcipvgiggd htttypvlra ikekhgpvgl lhldahadvn
      181 ptmmhcelah gtpfyravqd glldtrrvvq iglrgsvygp neerwslqhg fhiiqaqecw
      241 fksmapimet kvlpvlgagp vyisfdidcl dpafapgtgt peiggltsaq alevirsckg
      301 lnivgadlve vsppydtsgn taltaanllf emicvlpgvk cld
//
LOCUS       XP_077503300             721 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113833 [Amblyomma americanum].
ACCESSION   XP_077503300
VERSION     XP_077503300.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647174.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..721
                     /product="uncharacterized protein LOC144113833"
                     /calculated_mol_wt=80648
     Region          <234..407
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     Region          <496..>589
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     CDS             1..721
                     /gene="LOC144113833"
                     /coded_by="XM_077647174.1:284..2449"
                     /db_xref="GeneID:144113833"
ORIGIN      
        1 maypttyyhr maphpyplya dmpgfrract asstndcwlc dvltswnsal rycrlnlree
       61 epgklclqtl rcvfladdva prdkaavhsa afllaylprq hkcirrislh gehlphqrgp
      121 lrlllgadww pqqpecnvqy leiseivdfe wnillcglgr vsalkcliie raiidndyvs
      181 kldqllaans rglqklviss sraetdgisd rliraisnct hlaelsfdgh ltttgvvdlg
      241 rlllsceniq klslrdqfgd ecrlallgal sifvktnasl melhyeslsl dvielldalk
      301 fnrtlkhlvl sgykhnqktf grehgaalga alatnsglrs lvishctfse lavedisagl
      361 alntslerfd lshcrvnfgv asalcrvlyi nrtlhmlvld lngeaiseri rfseqvaaan
      421 cyhrvgmrwd etsmpnfvna lqqpslcple chiessrfsi rslsqvcsal vsstveslsv
      481 afidsfltep rhhvheairk nqsivslslq edrissgaci fvakallenr tvtklslrin
      541 swhtgaaetl seilsknqtl eklllncshv dlwcvdvias glkenrtlth fsifderdna
      601 scstmavdec vdrnlsswnc avqfvlevci srrraeifes lqgmpcflqr vaaasgksle
      661 eaatmvetak cfirsnylfi tgvvcrgicc ypsgqmqleh lysdcwhais kylrvsdvir
      721 h
//
LOCUS       XP_077503301             721 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113835 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503301
VERSION     XP_077503301.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647175.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..721
                     /product="uncharacterized protein LOC144113835 isoform X1"
                     /calculated_mol_wt=80677
     Region          193..221
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          222..248
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          249..279
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          <259..558
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     Region          280..304
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          338..365
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          <506..661
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     CDS             1..721
                     /gene="LOC144113835"
                     /coded_by="XM_077647175.1:293..2458"
                     /db_xref="GeneID:144113835"
ORIGIN      
        1 maypttyyhw maphpyplya dmpgfrract asstndcwlc dvltswnsal rycrlnlree
       61 epgklclqtl rcvfladdva prdkaavhsa afllaylprq hkcirrislh gehlphqrgp
      121 lrlllgadww pqqpecnvqy leiseivdfe wnillcglgr vsalkcliie raiidndyvs
      181 kldqllaans rglqklviss sraetdgisd rliraisnct hltelsfdgh ltttgvvdlg
      241 rlllsceniq klslrdqfgd ecrlallgal sifvktnasl melhyeslsl dviellnalk
      301 fnrtlkhlvl sgykhnqktf grehgaalga alatnsglrs lvishctfse lavedisagl
      361 alntslerfd lshcrvnfgv asalcrvlyi nrtlhmlvld lngeaiseri rfseqvaaan
      421 cyhrvgmrwd etsmpnfvna lqqpslcple chiessrfsi rslsqvcsal vsstveslsv
      481 afidsfltep rhhvheairk nqsivslslq edrissgaci fvakallenr tvtklslrin
      541 gwhtgaaetl seilsknqtl eklllncshv dlwcvdvias glkenrtlth fsifderdna
      601 scstmavdec vdrnlsswnc avqfvlevci srrraeifes lqgmpcflqr vaaasgksle
      661 eaatmvetak cfirsnylfi tgvvcrgicc ypsgqmqleh lysdcwhais kylrvsdvir
      721 h
//
LOCUS       XP_077503302             395 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113835 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503302
VERSION     XP_077503302.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647176.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..395
                     /product="uncharacterized protein LOC144113835 isoform X2"
                     /calculated_mol_wt=44334
     Region          <170..>263
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     CDS             1..395
                     /gene="LOC144113835"
                     /coded_by="XM_077647176.1:1879..3066"
                     /db_xref="GeneID:144113835"
ORIGIN      
        1 mlpfrttgqe gkeraeklai irlvfatvrv trsatddeva raladwlrfs nsrltaakfh
       61 rhtvgsvati fswhqpepyh lplirfseqv aaancyhrvg mrwdetsmpn fvnalqqpsl
      121 cplechiess rfsirslsqv csalvsstve slsvafidsf lteprhhvhe airknqsivs
      181 lslqedriss gacifvakal lenrtvtkls lringwhtga aetlseilsk nqtlekllln
      241 cshvdlwcvd viasglkenr tlthfsifde rdnascstma vdecvdrnls swncavqfvl
      301 evcisrrrae ifeslqgmpc flqrvaaasg ksleeaatmv etakcfirsn ylfitgvvcr
      361 giccypsgqm qlehlysdcw haiskylrvs dvirh
//
LOCUS       XP_077503303             334 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113836 [Amblyomma americanum].
ACCESSION   XP_077503303
VERSION     XP_077503303.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647177.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..334
                     /product="uncharacterized protein LOC144113836"
                     /calculated_mol_wt=38731
     CDS             1..334
                     /gene="LOC144113836"
                     /coded_by="XM_077647177.1:233..1237"
                     /db_xref="GeneID:144113836"
ORIGIN      
        1 maptgkrrsr rhprdvpael letrkqqtsa alrvtaerlt rpaercflnq reflrasrna
       61 ephpirleri rvaerarltr wspslspppn prrssrlrfr kratccspsl lpsssdasha
      121 pcekrrrclr apgahatnre wspeterrer livsrtlael hrslaekrhl lqqngksvls
      181 kvrsylqdva lkaqreeqee ekeediafsr vsreagreeq eeekeediaf llvsreagre
      241 eqeeekeedi assrvsgeag reeqeeekee diaflrvsre agreeqeeek eediassrvs
      301 geagreeqee ekeediafsr vsrearreeq chit
//
LOCUS       XP_077503305             300 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113837 [Amblyomma americanum].
ACCESSION   XP_077503305
VERSION     XP_077503305.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647179.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..300
                     /product="uncharacterized protein LOC144113837"
                     /calculated_mol_wt=34573
     CDS             1..300
                     /gene="LOC144113837"
                     /coded_by="XM_077647179.1:238..1140"
                     /db_xref="GeneID:144113837"
ORIGIN      
        1 maptgkrrsr rhprdvpael letrkqqtsa alrvtaerlt rpaercflnq reflrasrna
       61 ephpirleri rvaerarltr wspslspppn prrssrlrfr kratccspsl lpsssdasha
      121 pcekrrrclr apgahatnre wspeterrer livsrtlael hrslaekrhl lqqngksvls
      181 kvrsylqdva lkaqreeqee ekeediaflr vsreagreeq eeekeedias srvsgeagre
      241 eqeeekeedi assrvsgeag reeqeeekee diafsrvsge agreeqeeek eediaflrvt
//
LOCUS       XP_077503306             298 aa            linear   INV 25-NOV-2025
DEFINITION  transmembrane protein 231-like [Amblyomma americanum].
ACCESSION   XP_077503306
VERSION     XP_077503306.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647180.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..298
                     /product="transmembrane protein 231-like"
                     /calculated_mol_wt=34165
     Region          1..296
                     /region_name="TM231"
                     /note="Transmembrane protein 231; pfam10149"
                     /db_xref="CDD:462964"
     CDS             1..298
                     /gene="LOC144113838"
                     /coded_by="XM_077647180.1:30..926"
                     /db_xref="GeneID:144113838"
ORIGIN      
        1 maaytlfcka vyrnyraslc skatvfvvvi tgttiiipfi faykdlgfwk kidqyqeqps
       61 vrfkrqlifi legseglvyw ssypmlnlhv eglsvpsied leedfdsdgk ldelilsikm
      121 plprsfgvln mkllllfdck ietytkvsye glafvdhtss ipgsglsvvg elrlhqnell
      181 rrgshdhrfe hsiiegdssa vstfnldhil deyskrnvtt qlssvqstwk tasgaahfla
      241 nlrikypvei lwyrpgmwqv lkhawtqyla flaiflflge rtkefvfvnq iletyqlf
//
LOCUS       XP_077503307             298 aa            linear   INV 25-NOV-2025
DEFINITION  transmembrane protein 231-like [Amblyomma americanum].
ACCESSION   XP_077503307
VERSION     XP_077503307.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647181.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..298
                     /product="transmembrane protein 231-like"
                     /calculated_mol_wt=34165
     Region          1..296
                     /region_name="TM231"
                     /note="Transmembrane protein 231; pfam10149"
                     /db_xref="CDD:462964"
     CDS             1..298
                     /gene="LOC144113839"
                     /coded_by="XM_077647181.1:31..927"
                     /db_xref="GeneID:144113839"
ORIGIN      
        1 maaytlfcka vyrnyraslc skatvfvvvi tgttiiipfi faykdlgfwk kidqyqeqps
       61 vrfkrqlifi legseglvyw ssypmlnlhv eglsvpsied leedfdsdgk ldelilsikm
      121 plprsfgvln mkllllfdck ietytkvsye glafvdhtss ipgsglsvvg elrlhqnell
      181 rrgshdhrfe hsiiegdssa vstfnldhil deyskrnvtt qlssvqstwk tasgaahfla
      241 nlrikypvei lwyrpgmwqv lkhawtqyla flaiflflge rtkefvfvnq iletyqlf
//
LOCUS       XP_077503308             274 aa            linear   INV 25-NOV-2025
DEFINITION  dystrobrevin binding protein dysbindin isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503308
VERSION     XP_077503308.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647182.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..274
                     /product="dystrobrevin binding protein dysbindin isoform
                     X1"
                     /calculated_mol_wt=31580
     Region          57..>270
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <138..>234
                     /region_name="PRK00409"
                     /note="recombination and DNA strand exchange inhibitor
                     protein; Reviewed"
                     /db_xref="CDD:234750"
     CDS             1..274
                     /gene="Dysb"
                     /coded_by="XM_077647182.1:234..1058"
                     /db_xref="GeneID:144113840"
ORIGIN      
        1 mlenlrdrly svqqdfsfsl rnlslgdtsl pksqqecekr llnagadflh yfhlhfsyqs
       61 kweelhsqne gnakraeevd aliaglqsyc hqqlvsvqql nlqlaqlpkl qqgvqdlmnt
      121 igelegmfee velrlvsled vietqalqek qlderfklal ygekkkghfe slkekleedh
      181 qtllrqleeq erevlrerqe afgevfrrdl eaykeqgtlh rieavqdarv dledidlegn
      241 deerealdef ladvisttdg saaveestee iaaq
//
LOCUS       XP_077503309             274 aa            linear   INV 25-NOV-2025
DEFINITION  dystrobrevin binding protein dysbindin isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503309
VERSION     XP_077503309.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647183.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..274
                     /product="dystrobrevin binding protein dysbindin isoform
                     X2"
                     /calculated_mol_wt=31580
     Region          57..>270
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <138..>234
                     /region_name="PRK00409"
                     /note="recombination and DNA strand exchange inhibitor
                     protein; Reviewed"
                     /db_xref="CDD:234750"
     CDS             1..274
                     /gene="Dysb"
                     /coded_by="XM_077647183.1:236..1060"
                     /db_xref="GeneID:144113840"
ORIGIN      
        1 mlenlrdrly svqqdfsfsl rnlslgdtsl pksqqecekr llnagadflh yfhlhfsyqs
       61 kweelhsqne gnakraeevd aliaglqsyc hqqlvsvqql nlqlaqlpkl qqgvqdlmnt
      121 igelegmfee velrlvsled vietqalqek qlderfklal ygekkkghfe slkekleedh
      181 qtllrqleeq erevlrerqe afgevfrrdl eaykeqgtlh rieavqdarv dledidlegn
      241 deerealdef ladvisttdg saaveestee iaaq
//
LOCUS       XP_077503310             268 aa            linear   INV 25-NOV-2025
DEFINITION  dystrobrevin binding protein dysbindin isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503310
VERSION     XP_077503310.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647184.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..268
                     /product="dystrobrevin binding protein dysbindin isoform
                     X3"
                     /calculated_mol_wt=30811
     Region          <34..>268
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          <132..>228
                     /region_name="PRK00409"
                     /note="recombination and DNA strand exchange inhibitor
                     protein; Reviewed"
                     /db_xref="CDD:234750"
     CDS             1..268
                     /gene="Dysb"
                     /coded_by="XM_077647184.1:264..1070"
                     /db_xref="GeneID:144113840"
ORIGIN      
        1 mlenlrdrly svqqdfsfsl rnlslgdtsl pksqqecekr llnagadflh yyqskweelh
       61 sqnegnakra eevdaliagl qsychqqlvs vqqlnlqlaq lpklqqgvqd lmntigeleg
      121 mfeevelrlv sledvietqa lqekqlderf klalygekkk ghfeslkekl eedhqtllrq
      181 leeqerevlr erqeafgevf rrdleaykeq gtlhrieavq darvdledid legndeerea
      241 ldefladvis ttdgsaavee steeiaaq
//
LOCUS       XP_077503311             123 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113842 [Amblyomma americanum].
ACCESSION   XP_077503311
VERSION     XP_077503311.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647185.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..123
                     /product="uncharacterized protein LOC144113842"
                     /calculated_mol_wt=13142
     CDS             1..123
                     /gene="LOC144113842"
                     /coded_by="XM_077647185.1:34..405"
                     /db_xref="GeneID:144113842"
ORIGIN      
        1 mlqnilskhf clncslnash klstgnifta tkevlqdfls rpqtasgvfm dgnvsfcyvt
       61 edmaasmtat vdclpviips gdaffcspaf nsaiyfsasr thslgrlalf veivtttmta
      121 iea
//
LOCUS       XP_077503312             105 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113843 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503312
VERSION     XP_077503312.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647186.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..105
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..105
                     /product="uncharacterized protein LOC144113843 isoform X1"
                     /calculated_mol_wt=11961
     CDS             1..105
                     /gene="LOC144113843"
                     /coded_by="XM_077647186.1:2251..2568"
                     /db_xref="GeneID:144113843"
ORIGIN      
        1 mlpfrttgqe rkeraeklai irlvfatvrv trsatddeva raladwlrfs nsrltaakfh
       61 rhtvgsvati fswhqpepyh lplwvpkpdg rlqekyfasr aaskt
//
LOCUS       XP_077503313              78 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113843 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503313
VERSION     XP_077503313.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647187.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..78
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..78
                     /product="uncharacterized protein LOC144113843 isoform X2"
                     /calculated_mol_wt=8604
     CDS             1..78
                     /gene="LOC144113843"
                     /coded_by="XM_077647187.1:1220..1456"
                     /db_xref="GeneID:144113843"
ORIGIN      
        1 mlpsstkgst rhssgnslla eysqtqvrva kisshltqim phrhlrllvs qedqvvcflk
       61 ttvihvrqlw skfsgsss
//
LOCUS       XP_077503315             353 aa            linear   INV 25-NOV-2025
DEFINITION  queuosine 5'-phosphate N-glycosylase/hydrolase isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077503315
VERSION     XP_077503315.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647189.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..353
                     /product="queuosine 5'-phosphate N-glycosylase/hydrolase
                     isoform X1"
                     /calculated_mol_wt=40115
     Region          53..353
                     /region_name="Q_salvage"
                     /note="Potential Queuosine, Q, salvage protein family;
                     pfam10343"
                     /db_xref="CDD:463052"
     CDS             1..353
                     /gene="LOC144113848"
                     /coded_by="XM_077647189.1:292..1353"
                     /db_xref="GeneID:144113848"
ORIGIN      
        1 msvlspkdsa elivknardv tidpngiskv snvvldcynr glvgthawrd hplhpkvadd
       61 taaqwvflvd alnfnfwadn saeyfavrwn galhsgyysl cavvaralee gvpilsadfc
      121 rsislqqvqh ifrsdtstdi plleerhrvl qewghvlssm ydgsfancir ecnkwcqvll
      181 rmvvknftsf qdegtfeglk vsfykraqil vadlwscfng qglgefkdig tltafadyrv
      241 pqvlayfgal rysprltall akgnlfqngs keemeirgat ihacesmqll etlaeetkds
      301 snsqlvrkel kkrklpsaei nsvlvdyclw dyrrrhenev ehvpfhrvrc iyy
//
LOCUS       XP_077503316             353 aa            linear   INV 25-NOV-2025
DEFINITION  queuosine 5'-phosphate N-glycosylase/hydrolase isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077503316
VERSION     XP_077503316.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647190.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..353
                     /product="queuosine 5'-phosphate N-glycosylase/hydrolase
                     isoform X1"
                     /calculated_mol_wt=40115
     Region          53..353
                     /region_name="Q_salvage"
                     /note="Potential Queuosine, Q, salvage protein family;
                     pfam10343"
                     /db_xref="CDD:463052"
     CDS             1..353
                     /gene="LOC144113848"
                     /coded_by="XM_077647190.1:141..1202"
                     /db_xref="GeneID:144113848"
ORIGIN      
        1 msvlspkdsa elivknardv tidpngiskv snvvldcynr glvgthawrd hplhpkvadd
       61 taaqwvflvd alnfnfwadn saeyfavrwn galhsgyysl cavvaralee gvpilsadfc
      121 rsislqqvqh ifrsdtstdi plleerhrvl qewghvlssm ydgsfancir ecnkwcqvll
      181 rmvvknftsf qdegtfeglk vsfykraqil vadlwscfng qglgefkdig tltafadyrv
      241 pqvlayfgal rysprltall akgnlfqngs keemeirgat ihacesmqll etlaeetkds
      301 snsqlvrkel kkrklpsaei nsvlvdyclw dyrrrhenev ehvpfhrvrc iyy
//
LOCUS       XP_077503317             334 aa            linear   INV 25-NOV-2025
DEFINITION  queuosine 5'-phosphate N-glycosylase/hydrolase isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077503317
VERSION     XP_077503317.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647191.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..334
                     /product="queuosine 5'-phosphate N-glycosylase/hydrolase
                     isoform X2"
                     /calculated_mol_wt=38022
     Region          53..334
                     /region_name="Q_salvage"
                     /note="Potential Queuosine, Q, salvage protein family;
                     pfam10343"
                     /db_xref="CDD:463052"
     CDS             1..334
                     /gene="LOC144113848"
                     /coded_by="XM_077647191.1:288..1292"
                     /db_xref="GeneID:144113848"
ORIGIN      
        1 msvlspkdsa elivknardv tidpngiskv snvvldcynr glvgthawrd hplhpkvadd
       61 taaqwvflvd alnfnfwadn saeyfavrwn galhsgyysl cavvaralee gvpilsadfc
      121 rsislqqvqh ifrsdtstdi plleerhrvl qewghvlssm ydgsfancir ecnkwcqvll
      181 rmvvknftsf qdegtfeglk vsfykraqil vadlwscfng qglgefkdig tltafadyrv
      241 pqvlayfgal rysprltall akgnlfqngs keemeirgat ihacelvrke lkkrklpsae
      301 insvlvdycl wdyrrrhene vehvpfhrvr ciyy
//
LOCUS       XP_077503318             334 aa            linear   INV 25-NOV-2025
DEFINITION  queuosine 5'-phosphate N-glycosylase/hydrolase isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077503318
VERSION     XP_077503318.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647192.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..334
                     /product="queuosine 5'-phosphate N-glycosylase/hydrolase
                     isoform X2"
                     /calculated_mol_wt=38022
     Region          53..334
                     /region_name="Q_salvage"
                     /note="Potential Queuosine, Q, salvage protein family;
                     pfam10343"
                     /db_xref="CDD:463052"
     CDS             1..334
                     /gene="LOC144113848"
                     /coded_by="XM_077647192.1:141..1145"
                     /db_xref="GeneID:144113848"
ORIGIN      
        1 msvlspkdsa elivknardv tidpngiskv snvvldcynr glvgthawrd hplhpkvadd
       61 taaqwvflvd alnfnfwadn saeyfavrwn galhsgyysl cavvaralee gvpilsadfc
      121 rsislqqvqh ifrsdtstdi plleerhrvl qewghvlssm ydgsfancir ecnkwcqvll
      181 rmvvknftsf qdegtfeglk vsfykraqil vadlwscfng qglgefkdig tltafadyrv
      241 pqvlayfgal rysprltall akgnlfqngs keemeirgat ihacelvrke lkkrklpsae
      301 insvlvdycl wdyrrrhene vehvpfhrvr ciyy
//
LOCUS       XP_077503319             274 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113851 [Amblyomma americanum].
ACCESSION   XP_077503319
VERSION     XP_077503319.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647193.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..274
                     /product="uncharacterized protein LOC144113851"
                     /calculated_mol_wt=29976
     Region          20..100
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     Region          101..>233
                     /region_name="flhF"
                     /note="flagellar biosynthesis protein FlhF; PRK06995"
                     /db_xref="CDD:235904"
     CDS             1..274
                     /gene="LOC144113851"
                     /coded_by="XM_077647193.1:1..825"
                     /db_xref="GeneID:144113851"
ORIGIN      
        1 malnfsgddy gsfkgrlgrg cswtreetll lldlyekerl tderadfpph ntpkyqkvhq
       61 aisgflgahg ysrtpmqvre rlkrlkrdfr enrhgeftdr vgtilakraa lgapasppng
      121 svaatvagpq sspehsleac cpavlvktel gapeqpqqgs tlrkprtdwq plpiagvrap
      181 epsgkvekqr ptlseeqaae rpaassnggp rarkslqqrv vrllgqllae srrqtqaaea
      241 fqqqllyqmh liscslqvla agspqgplkr ddss
//
LOCUS       XP_077503320             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113852 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503320
VERSION     XP_077503320.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647194.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144113852 isoform X1"
                     /calculated_mol_wt=13868
     CDS             1..127
                     /gene="LOC144113852"
                     /coded_by="XM_077647194.1:23..406"
                     /db_xref="GeneID:144113852"
ORIGIN      
        1 mrltlllhmq vfftlgtsav lgptgqamsp cliepfssaa sgwkltvvst tvdkrgspvi
       61 nvsklhsdtd sidcdppiff ehqeprdynq aylnplrfgy vgfgagtlad mrltlllhmq
      121 ivagwnr
//
LOCUS       XP_077503322             100 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113852 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503322
VERSION     XP_077503322.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647196.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..100
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..100
                     /product="uncharacterized protein LOC144113852 isoform X2"
                     /calculated_mol_wt=10983
     CDS             1..100
                     /gene="LOC144113852"
                     /coded_by="XM_077647196.1:1395..1697"
                     /db_xref="GeneID:144113852"
ORIGIN      
        1 mspcliepfs saasgwkltv vsttvdkrgs pvinvsklhs dtdsidcdpp iffehqeprd
       61 ynqaylnplr fgyvgfgagt ladmrltlll hmqivagwnr
//
LOCUS       XP_077503323            2072 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113853 [Amblyomma americanum].
ACCESSION   XP_077503323
VERSION     XP_077503323.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647197.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2072
                     /product="uncharacterized protein LOC144113853"
                     /calculated_mol_wt=233338
     Region          281..302
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          310..330
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(310,313,326,330)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(315,317,319,321..322,325..326,329,342,344,348..349,
                     352..353,356,370,372,374,376..377,380..381)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          337..357
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          365..381
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          560..580
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Site            order(565,567,569,571..572,575..576,579,593,595..597,
                     599..600,604,608)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275370"
     Region          586..610
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          588..609
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          693..713
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Site            order(698,700,702,704..705,708..709,712,726,728..730,
                     732..733,737)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275370"
     Region          721..739
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          1039..1059
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1039,1042,1055,1059)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1044,1045,1047,1049..1050,1054..1055,1058,1098,1100,
                     1104..1105,1108..1109,1112,1128,1130,1132,1134..1135,
                     1138..1139,1142)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1093..1113
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1123..1143
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1209..1229
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1209,1212,1225,1229)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          1237..1257
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..2072
                     /gene="LOC144113853"
                     /coded_by="XM_077647197.1:307..6525"
                     /db_xref="GeneID:144113853"
ORIGIN      
        1 mkpgfltmps yrcesctfvg knrkhlanhv alnhssrvyr cslcafvtry qanfyrhrrs
       61 ihklnsanvc emcgqlcdnq delvahtsne hpqfldvllr kleakrvrya kyaakmnmed
      121 gdtarasaek ivannlepel vtgdvsdqih lqngvpeeqn esveqskkrp ressppvsnk
      181 kgrtgrrtyt caecdlvtkw preflshrrd vhgdrisihe cpfceyaskq pqklqrhcni
      241 vhkeemygek srhlvktgpp asspapsaei kvhiqekvfk csscnfhaks kihveehekt
      301 vhlkrrfyrc qqcgyvchek grytrhmrfh slpkikcqfc nfqtcykwnm drhlkyhtsg
      361 gefrcnkcgf mtmsrkslta hclhhhnell kdatdsegen telenvddde drlmidedaa
      421 edstsppplk mtlkrtgeia vvensegtmr kcqycpqqvl wpselkrhea thfkmnkkhg
      481 cplchirfdq ldhlehhvml dhqeeasdks ssdavatgtt sapiiielgk sddstsrany
      541 sgstqaldis ikskqpmhvc qrcgyttrwi selrkhelih gnlkpfkcty csytsrwkgd
      601 mtrhvfrmhk entgaadtns nedpehakss hvlrfllsqp knadhdydsk aaepslskds
      661 ealsgkflpk ppesqcsfqv pvgiprivqk ykcpqcsflt rtasrfhvhm iqhlnkrpfm
      721 csvcnyrsnw qwdvnkhiks ksgndpkhkk aelvvmdetg fknyakykih lvdveesskg
      781 dkvlqpaqps rlnqeevqli rpdetenivv tpdilygdqd yqddlgapip esssnqqvly
      841 cghcnfnhte rkavlshlgs hasvkpyrcr lcnfvskwyh ivlmhvrhrh ntgpkhiesn
      901 vsfveegntf rlkeeeepav ppapqnaepk ifkcntcpyr cskvchmefh mkqhvpkega
      961 iykckycpyy anvkktlsrh mklhkeeqqp atevlprllv kadmkkhace hcpytsdnkt
     1021 qylyhkqfhr pnknaphkct hcnywsthsh lmaqhqkihr sadgrpldga pysggiqtmt
     1081 tmvngtahrm fkcrfcpltn krranvkihe rmhtaskgak fqcvlcsyrc nntgvlashm
     1141 klhkadnpsl dlsrmktfce nlnalpsrpa vakksvpesa aaaaqrseis alkdklktps
     1201 rkkifsyfcd kcpamfksht dldthktyhn sahlypchlc dyrarhkphl hkhllvhtpe
     1261 yaerqngftl aeirsqdrsq qpesatppaa paaadqmlll ekaetrafvd agsqsmgvql
     1321 hrcircpaaf qkaatleyhl slhgsagvya chfcnyaans aanvsahthl hyrgalkqkq
     1381 ppkmfrcdkc pasfskynrf eshltlhgrn erfrcshcdy svkfaanlvk hrklhevlvp
     1441 eskqpkvepp apltvtstpq plengsepst avplsdsapn aatpavveek rvyicskcpy
     1501 afhrretvvn hlqrhgtseg lcctfcdyrs vhmstlrdht rchfqpmrhy kpqaymkcds
     1561 leiwstasdg trtlvfrddg dekyfpeldd cddageddtl sspastpass gscsastass
     1621 spqlqlnnkd lepavlksvk letddvvlas ketleelvsn vvvlndekee mppgcktqvv
     1681 nivvldgtkq lnevgevaqa ehksesetdf kddgesaqdl fnricnpdsm eyedledekd
     1741 vycetlsedp dvadevecet veedsiepea laadadpmlk apskhaghte flditeaadc
     1801 evsaeckgmf lhsveceseh mtadvpdgva tsslvtdksl aavietgvts esdiscqvkk
     1861 feeskpvcsr idsedhpvat flndspcvgl keaavsnlan nfelkdcgve vagynlepdt
     1921 csraktqdvh ddfsqpnvlq sssgcsympv sqgvisitvc kssedsctae ndctqdgegt
     1981 qvlhsvsssl gadahcqrav eeieadeisk crsdpdamsc ivpddvveie igsslptika
     2041 dpggdtvlvp qevfemqspt glqrsilvpe ld
//
LOCUS       XP_077503324            2072 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113853 [Amblyomma americanum].
ACCESSION   XP_077503324
VERSION     XP_077503324.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647198.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2072
                     /product="uncharacterized protein LOC144113853"
                     /calculated_mol_wt=233338
     Region          281..302
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          310..330
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(310,313,326,330)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(315,317,319,321..322,325..326,329,342,344,348..349,
                     352..353,356,370,372,374,376..377,380..381)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          337..357
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          365..381
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          560..580
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Site            order(565,567,569,571..572,575..576,579,593,595..597,
                     599..600,604,608)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275370"
     Region          586..610
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          588..609
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          693..713
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Site            order(698,700,702,704..705,708..709,712,726,728..730,
                     732..733,737)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275370"
     Region          721..739
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          1039..1059
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1039,1042,1055,1059)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(1044,1045,1047,1049..1050,1054..1055,1058,1098,1100,
                     1104..1105,1108..1109,1112,1128,1130,1132,1134..1135,
                     1138..1139,1142)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1093..1113
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1123..1143
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1209..1229
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1209,1212,1225,1229)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          1237..1257
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..2072
                     /gene="LOC144113853"
                     /coded_by="XM_077647198.1:305..6523"
                     /db_xref="GeneID:144113853"
ORIGIN      
        1 mkpgfltmps yrcesctfvg knrkhlanhv alnhssrvyr cslcafvtry qanfyrhrrs
       61 ihklnsanvc emcgqlcdnq delvahtsne hpqfldvllr kleakrvrya kyaakmnmed
      121 gdtarasaek ivannlepel vtgdvsdqih lqngvpeeqn esveqskkrp ressppvsnk
      181 kgrtgrrtyt caecdlvtkw preflshrrd vhgdrisihe cpfceyaskq pqklqrhcni
      241 vhkeemygek srhlvktgpp asspapsaei kvhiqekvfk csscnfhaks kihveehekt
      301 vhlkrrfyrc qqcgyvchek grytrhmrfh slpkikcqfc nfqtcykwnm drhlkyhtsg
      361 gefrcnkcgf mtmsrkslta hclhhhnell kdatdsegen telenvddde drlmidedaa
      421 edstsppplk mtlkrtgeia vvensegtmr kcqycpqqvl wpselkrhea thfkmnkkhg
      481 cplchirfdq ldhlehhvml dhqeeasdks ssdavatgtt sapiiielgk sddstsrany
      541 sgstqaldis ikskqpmhvc qrcgyttrwi selrkhelih gnlkpfkcty csytsrwkgd
      601 mtrhvfrmhk entgaadtns nedpehakss hvlrfllsqp knadhdydsk aaepslskds
      661 ealsgkflpk ppesqcsfqv pvgiprivqk ykcpqcsflt rtasrfhvhm iqhlnkrpfm
      721 csvcnyrsnw qwdvnkhiks ksgndpkhkk aelvvmdetg fknyakykih lvdveesskg
      781 dkvlqpaqps rlnqeevqli rpdetenivv tpdilygdqd yqddlgapip esssnqqvly
      841 cghcnfnhte rkavlshlgs hasvkpyrcr lcnfvskwyh ivlmhvrhrh ntgpkhiesn
      901 vsfveegntf rlkeeeepav ppapqnaepk ifkcntcpyr cskvchmefh mkqhvpkega
      961 iykckycpyy anvkktlsrh mklhkeeqqp atevlprllv kadmkkhace hcpytsdnkt
     1021 qylyhkqfhr pnknaphkct hcnywsthsh lmaqhqkihr sadgrpldga pysggiqtmt
     1081 tmvngtahrm fkcrfcpltn krranvkihe rmhtaskgak fqcvlcsyrc nntgvlashm
     1141 klhkadnpsl dlsrmktfce nlnalpsrpa vakksvpesa aaaaqrseis alkdklktps
     1201 rkkifsyfcd kcpamfksht dldthktyhn sahlypchlc dyrarhkphl hkhllvhtpe
     1261 yaerqngftl aeirsqdrsq qpesatppaa paaadqmlll ekaetrafvd agsqsmgvql
     1321 hrcircpaaf qkaatleyhl slhgsagvya chfcnyaans aanvsahthl hyrgalkqkq
     1381 ppkmfrcdkc pasfskynrf eshltlhgrn erfrcshcdy svkfaanlvk hrklhevlvp
     1441 eskqpkvepp apltvtstpq plengsepst avplsdsapn aatpavveek rvyicskcpy
     1501 afhrretvvn hlqrhgtseg lcctfcdyrs vhmstlrdht rchfqpmrhy kpqaymkcds
     1561 leiwstasdg trtlvfrddg dekyfpeldd cddageddtl sspastpass gscsastass
     1621 spqlqlnnkd lepavlksvk letddvvlas ketleelvsn vvvlndekee mppgcktqvv
     1681 nivvldgtkq lnevgevaqa ehksesetdf kddgesaqdl fnricnpdsm eyedledekd
     1741 vycetlsedp dvadevecet veedsiepea laadadpmlk apskhaghte flditeaadc
     1801 evsaeckgmf lhsveceseh mtadvpdgva tsslvtdksl aavietgvts esdiscqvkk
     1861 feeskpvcsr idsedhpvat flndspcvgl keaavsnlan nfelkdcgve vagynlepdt
     1921 csraktqdvh ddfsqpnvlq sssgcsympv sqgvisitvc kssedsctae ndctqdgegt
     1981 qvlhsvsssl gadahcqrav eeieadeisk crsdpdamsc ivpddvveie igsslptika
     2041 dpggdtvlvp qevfemqspt glqrsilvpe ld
//
LOCUS       XP_077503325             778 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113854 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503325
VERSION     XP_077503325.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647199.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..778
                     /product="uncharacterized protein LOC144113854 isoform X1"
                     /calculated_mol_wt=88542
     Region          509..529
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Site            order(514,516,518,520..521,524..525,528,542,544..546,
                     548..549,553,556)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275370"
     Region          535..558
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          537..557
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          640..660
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Site            order(645,647,649,651..652,655..656,659,673,675..677,
                     679..680,684)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275370"
     Region          668..686
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     CDS             1..778
                     /gene="LOC144113854"
                     /coded_by="XM_077647199.1:2194..4530"
                     /db_xref="GeneID:144113854"
ORIGIN      
        1 mkpgfptmps yrcescpfvg knrkhlsnhv alnhssrvyr cslcafvtry qaslsrhrrs
       61 ihkinsanvc emcgqlcdnq delvahtsne hpqfsdvllr kleakrvrya kyaakmnred
      121 gdtarasvek ivannlepel vtgdvsdqih lqngvpeeqn esverskkrp hessppvsnk
      181 kgrtgrrtyt cakcdlvtkw preflshrrd vhgdhisihe cpfceyaskk lyfikrhcnv
      241 vhkeemyven srhlfktass asspapsaei nvniqekvfk cnscnfiakr kihveehekk
      301 ghlkrqssdg efhcskcsfm tmsrkslsah clqhhnevlk datssegent ereivddded
      361 rlvidedaae dstsppslkm tlkqteeiav veniegtmwk crycpqqvlw pselkrheet
      421 hfkmnkkhgc llchirfdql nhlehhvmln hqeepsdkss sdavatgkts asiiielgks
      481 ddstshasys gstqahdisi kskqpihvcq hcgyitrwis glrkhelthe nlkpfkcayc
      541 sytsrwnsdl krhakkhnen tgaadtnpne dpehaksshv mhlllepkna dhdydskaae
      601 pslskgseap srkllpkpae sqcsfevpvg iprivqkykc pqcsfltrta shfhthmiqh
      661 lnkkpfmcsv cnyrsnwqfe vnkhikgksg ndaehkkael vvidetgfkn yakykihlvd
      721 vkesskgdkv lqpaqpsrln qeevqlirpd etenivvtpd ilygdqdyqd dlgapipe
//
LOCUS       XP_077503326             109 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113854 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503326
VERSION     XP_077503326.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647200.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..109
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..109
                     /product="uncharacterized protein LOC144113854 isoform X2"
                     /calculated_mol_wt=12007
     CDS             1..109
                     /gene="LOC144113854"
                     /coded_by="XM_077647200.1:1367..1696"
                     /db_xref="GeneID:144113854"
ORIGIN      
        1 mapwkvqras hpltcccemg sdgrtaaqae rprlsklrra gtgrtdecsp avgadcpgqp
       61 srneeafatq dkfhrlciyr rlpgpascps palrvaefrt rwiielilr
//
LOCUS       XP_077503327             278 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113855 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503327
VERSION     XP_077503327.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647201.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..278
                     /product="uncharacterized protein LOC144113855 isoform X1"
                     /calculated_mol_wt=31310
     Region          69..265
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(124,163,179,181)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..278
                     /gene="LOC144113855"
                     /coded_by="XM_077647201.1:461..1297"
                     /db_xref="GeneID:144113855"
ORIGIN      
        1 mdfklcgtrn svhkcwhcrn adstttvyes crkwqiqdgd fsaaeaailk glqvssqeai
       61 nhekntrqqs gnemwkrahv hrltassfgv avkrptwtek glqnvtadkd lsrvravqys
      121 itneqpavqr yvavmkardh dvealrsglv vdpscpwlga spdslifnpc etpshgllev
      181 kcpyslkgkt pddmgdghcl kkdsmgafrl drghdyyyqv lgrmalsgis wtdffsfsdr
      241 pliidrvkfs aedwavarqk ldnffftvll pyiaskrp
//
LOCUS       XP_077503328             278 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113855 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503328
VERSION     XP_077503328.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647202.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..278
                     /product="uncharacterized protein LOC144113855 isoform X1"
                     /calculated_mol_wt=31310
     Region          69..265
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(124,163,179,181)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..278
                     /gene="LOC144113855"
                     /coded_by="XM_077647202.1:583..1419"
                     /db_xref="GeneID:144113855"
ORIGIN      
        1 mdfklcgtrn svhkcwhcrn adstttvyes crkwqiqdgd fsaaeaailk glqvssqeai
       61 nhekntrqqs gnemwkrahv hrltassfgv avkrptwtek glqnvtadkd lsrvravqys
      121 itneqpavqr yvavmkardh dvealrsglv vdpscpwlga spdslifnpc etpshgllev
      181 kcpyslkgkt pddmgdghcl kkdsmgafrl drghdyyyqv lgrmalsgis wtdffsfsdr
      241 pliidrvkfs aedwavarqk ldnffftvll pyiaskrp
//
LOCUS       XP_077503329             278 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113855 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503329
VERSION     XP_077503329.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647203.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..278
                     /product="uncharacterized protein LOC144113855 isoform X1"
                     /calculated_mol_wt=31310
     Region          69..265
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(124,163,179,181)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..278
                     /gene="LOC144113855"
                     /coded_by="XM_077647203.1:606..1442"
                     /db_xref="GeneID:144113855"
ORIGIN      
        1 mdfklcgtrn svhkcwhcrn adstttvyes crkwqiqdgd fsaaeaailk glqvssqeai
       61 nhekntrqqs gnemwkrahv hrltassfgv avkrptwtek glqnvtadkd lsrvravqys
      121 itneqpavqr yvavmkardh dvealrsglv vdpscpwlga spdslifnpc etpshgllev
      181 kcpyslkgkt pddmgdghcl kkdsmgafrl drghdyyyqv lgrmalsgis wtdffsfsdr
      241 pliidrvkfs aedwavarqk ldnffftvll pyiaskrp
//
LOCUS       XP_077503330             278 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113855 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503330
VERSION     XP_077503330.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647204.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..278
                     /product="uncharacterized protein LOC144113855 isoform X1"
                     /calculated_mol_wt=31310
     Region          69..265
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(124,163,179,181)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..278
                     /gene="LOC144113855"
                     /coded_by="XM_077647204.1:342..1178"
                     /db_xref="GeneID:144113855"
ORIGIN      
        1 mdfklcgtrn svhkcwhcrn adstttvyes crkwqiqdgd fsaaeaailk glqvssqeai
       61 nhekntrqqs gnemwkrahv hrltassfgv avkrptwtek glqnvtadkd lsrvravqys
      121 itneqpavqr yvavmkardh dvealrsglv vdpscpwlga spdslifnpc etpshgllev
      181 kcpyslkgkt pddmgdghcl kkdsmgafrl drghdyyyqv lgrmalsgis wtdffsfsdr
      241 pliidrvkfs aedwavarqk ldnffftvll pyiaskrp
//
LOCUS       XP_077503331             278 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113855 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503331
VERSION     XP_077503331.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647205.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..278
                     /product="uncharacterized protein LOC144113855 isoform X1"
                     /calculated_mol_wt=31310
     Region          69..265
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(124,163,179,181)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..278
                     /gene="LOC144113855"
                     /coded_by="XM_077647205.1:601..1437"
                     /db_xref="GeneID:144113855"
ORIGIN      
        1 mdfklcgtrn svhkcwhcrn adstttvyes crkwqiqdgd fsaaeaailk glqvssqeai
       61 nhekntrqqs gnemwkrahv hrltassfgv avkrptwtek glqnvtadkd lsrvravqys
      121 itneqpavqr yvavmkardh dvealrsglv vdpscpwlga spdslifnpc etpshgllev
      181 kcpyslkgkt pddmgdghcl kkdsmgafrl drghdyyyqv lgrmalsgis wtdffsfsdr
      241 pliidrvkfs aedwavarqk ldnffftvll pyiaskrp
//
LOCUS       XP_077503332             278 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113855 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503332
VERSION     XP_077503332.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647206.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..278
                     /product="uncharacterized protein LOC144113855 isoform X1"
                     /calculated_mol_wt=31310
     Region          69..265
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(124,163,179,181)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..278
                     /gene="LOC144113855"
                     /coded_by="XM_077647206.1:517..1353"
                     /db_xref="GeneID:144113855"
ORIGIN      
        1 mdfklcgtrn svhkcwhcrn adstttvyes crkwqiqdgd fsaaeaailk glqvssqeai
       61 nhekntrqqs gnemwkrahv hrltassfgv avkrptwtek glqnvtadkd lsrvravqys
      121 itneqpavqr yvavmkardh dvealrsglv vdpscpwlga spdslifnpc etpshgllev
      181 kcpyslkgkt pddmgdghcl kkdsmgafrl drghdyyyqv lgrmalsgis wtdffsfsdr
      241 pliidrvkfs aedwavarqk ldnffftvll pyiaskrp
//
LOCUS       XP_077503333             266 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113855 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503333
VERSION     XP_077503333.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647207.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..266
                     /product="uncharacterized protein LOC144113855 isoform X2"
                     /calculated_mol_wt=29699
     Region          57..253
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(112,151,167,169)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..266
                     /gene="LOC144113855"
                     /coded_by="XM_077647207.1:171..971"
                     /db_xref="GeneID:144113855"
ORIGIN      
        1 msvslggppn mvprpaqrsd ehrrppvhgt ggyhgytlgl qvssqeainh ekntrqqsgn
       61 emwkrahvhr ltassfgvav krptwtekgl qnvtadkdls rvravqysit neqpavqryv
      121 avmkardhdv ealrsglvvd pscpwlgasp dslifnpcet pshgllevkc pyslkgktpd
      181 dmgdghclkk dsmgafrldr ghdyyyqvlg rmalsgiswt dffsfsdrpl iidrvkfsae
      241 dwavarqkld nffftvllpy iaskrp
//
LOCUS       XP_077503334             155 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113855 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503334
VERSION     XP_077503334.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647208.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..155
                     /product="uncharacterized protein LOC144113855 isoform X3"
                     /calculated_mol_wt=17460
     CDS             1..155
                     /gene="LOC144113855"
                     /coded_by="XM_077647208.1:175..642"
                     /db_xref="GeneID:144113855"
ORIGIN      
        1 msvslggppn mvprpaqrsd ehrrppvhgt ggyhgytlfv dwrsvrqggl skpittrfyd
       61 arqhaqpfed lssrirtlga sigfasiqpg ghqprkehea avwqrdvees prpsidcfii
      121 wgcgqapyvd rerpaerdrr qrpfpcqgcs vqhye
//
LOCUS       XP_077503335             121 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113855 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503335
VERSION     XP_077503335.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647209.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..121
                     /product="uncharacterized protein LOC144113855 isoform X4"
                     /calculated_mol_wt=13886
     CDS             1..121
                     /gene="LOC144113855"
                     /coded_by="XM_077647209.1:193..558"
                     /db_xref="GeneID:144113855"
ORIGIN      
        1 mpyefvdwrs vrqgglskpi ttrfydarqh aqpfedlssr irtlgasigf asiqpgghqp
       61 rkeheaavwq rdveesprps idcfiiwgcg qapyvdrerp aerdrrqrpf pcqgcsvqhy
      121 e
//
LOCUS       XP_077503336             121 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113855 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503336
VERSION     XP_077503336.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647210.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..121
                     /product="uncharacterized protein LOC144113855 isoform X4"
                     /calculated_mol_wt=13886
     CDS             1..121
                     /gene="LOC144113855"
                     /coded_by="XM_077647210.1:323..688"
                     /db_xref="GeneID:144113855"
ORIGIN      
        1 mpyefvdwrs vrqgglskpi ttrfydarqh aqpfedlssr irtlgasigf asiqpgghqp
       61 rkeheaavwq rdveesprps idcfiiwgcg qapyvdrerp aerdrrqrpf pcqgcsvqhy
      121 e
//
LOCUS       XP_077503337             121 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113855 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503337
VERSION     XP_077503337.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647211.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..121
                     /product="uncharacterized protein LOC144113855 isoform X4"
                     /calculated_mol_wt=13886
     CDS             1..121
                     /gene="LOC144113855"
                     /coded_by="XM_077647211.1:347..712"
                     /db_xref="GeneID:144113855"
ORIGIN      
        1 mpyefvdwrs vrqgglskpi ttrfydarqh aqpfedlssr irtlgasigf asiqpgghqp
       61 rkeheaavwq rdveesprps idcfiiwgcg qapyvdrerp aerdrrqrpf pcqgcsvqhy
      121 e
//
LOCUS       XP_077503338             121 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113855 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503338
VERSION     XP_077503338.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647212.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..121
                     /product="uncharacterized protein LOC144113855 isoform X4"
                     /calculated_mol_wt=13886
     CDS             1..121
                     /gene="LOC144113855"
                     /coded_by="XM_077647212.1:314..679"
                     /db_xref="GeneID:144113855"
ORIGIN      
        1 mpyefvdwrs vrqgglskpi ttrfydarqh aqpfedlssr irtlgasigf asiqpgghqp
       61 rkeheaavwq rdveesprps idcfiiwgcg qapyvdrerp aerdrrqrpf pcqgcsvqhy
      121 e
//
LOCUS       XP_077503339             121 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113855 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503339
VERSION     XP_077503339.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647213.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..121
                     /product="uncharacterized protein LOC144113855 isoform X4"
                     /calculated_mol_wt=13886
     CDS             1..121
                     /gene="LOC144113855"
                     /coded_by="XM_077647213.1:366..731"
                     /db_xref="GeneID:144113855"
ORIGIN      
        1 mpyefvdwrs vrqgglskpi ttrfydarqh aqpfedlssr irtlgasigf asiqpgghqp
       61 rkeheaavwq rdveesprps idcfiiwgcg qapyvdrerp aerdrrqrpf pcqgcsvqhy
      121 e
//
LOCUS       XP_077503340             235 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113857 [Amblyomma americanum].
ACCESSION   XP_077503340
VERSION     XP_077503340.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647214.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..235
                     /product="uncharacterized protein LOC144113857"
                     /calculated_mol_wt=25188
     Region          6..84
                     /region_name="GT1"
                     /note="myb-like, SANT family; cl23759"
                     /db_xref="CDD:474047"
     CDS             1..235
                     /gene="LOC144113857"
                     /coded_by="XM_077647214.1:457..1164"
                     /db_xref="GeneID:144113857"
ORIGIN      
        1 msektsarvs keqsllmmef meqhpalaha asplsggyta arrrelwqql aallnaagpv
       61 aksvlrwrht wqvwctrckk qeaqflaagr gtgggrlpgl agsvcklrsp glvlgvaapm
      121 fssssqnqpr vsvgempgts glqqaearsp satqeqpgvv aghpsqqqrs twteevhqll
      181 aetrrtndll earsvedvaf harlleeqrq tsaalrsltv avtqltaavt etgai
//
LOCUS       XP_077503341             149 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113858 [Amblyomma americanum].
ACCESSION   XP_077503341
VERSION     XP_077503341.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647215.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..149
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..149
                     /product="uncharacterized protein LOC144113858"
                     /calculated_mol_wt=16607
     CDS             1..149
                     /gene="LOC144113858"
                     /coded_by="XM_077647215.1:177..626"
                     /db_xref="GeneID:144113858"
ORIGIN      
        1 mpvhpqpqgc stvaargppg vlnlwllnra efifpmlpsn whlpckrsds gntwsaanfg
       61 krkksvalvd qrklqatnsd qhlpkqrshn vitgdgalty rernrrignv mkniaplhki
      121 kqrskvsgvl htpqftvhvh swtknlnig
//
LOCUS       XP_077503343             466 aa            linear   INV 25-NOV-2025
DEFINITION  ileal sodium/bile acid cotransporter-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503343
VERSION     XP_077503343.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647217.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..466
                     /product="ileal sodium/bile acid cotransporter-like
                     isoform X1"
                     /calculated_mol_wt=49599
     Region          120..405
                     /region_name="SBF"
                     /note="Sodium Bile acid symporter family; cl19217"
                     /db_xref="CDD:450274"
     CDS             1..466
                     /gene="LOC144113863"
                     /coded_by="XM_077647217.1:201..1601"
                     /db_xref="GeneID:144113863"
ORIGIN      
        1 mcpvvllall lsllllllev vaalsvafnp arvdklkqgs farvsfsapp leprffsvas
       61 dnpyvasvad lnftetgsfv ngtftvkanf lghakvrvvt ngtesteflp vsvvrkqskl
      121 sliftvsvav lvslnyinmg caldmevvkd vlrrpigpvv gffsqylamp lvaygmaqwl
      181 feeaylqlgl ftfgcspggg asnmwtvllg gnlnlsiamt fistiaslgt mplwlftlgr
      241 slfqeaslri pfrnifttli smtlplgvgl alqrwrpkva dvckrllapv svamivyivv
      301 fgtyanlymf qlmswqvlva aaanvwvgfa vgalatrcag lpwvdvlavs ietgvqntgi
      361 aivllgfslp qpeadiasvv pvaasivtpi pllllllvqk vysrccrrad tvadrkgvaa
      421 faevgaagda eeekraltvc ngalangtlg ggkaaeasyg deperl
//
LOCUS       XP_077503344             466 aa            linear   INV 25-NOV-2025
DEFINITION  ileal sodium/bile acid cotransporter-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503344
VERSION     XP_077503344.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647218.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..466
                     /product="ileal sodium/bile acid cotransporter-like
                     isoform X2"
                     /calculated_mol_wt=49442
     Region          120..405
                     /region_name="SBF"
                     /note="Sodium Bile acid symporter family; cl19217"
                     /db_xref="CDD:450274"
     CDS             1..466
                     /gene="LOC144113863"
                     /coded_by="XM_077647218.1:197..1597"
                     /db_xref="GeneID:144113863"
ORIGIN      
        1 mcpvvllall lsllllllev vaalsvafnp arvdklkqgs farvsfsapp leprffsvas
       61 dnpyvasvad lnftetgsfv ngtftvkanf lghakvrvvt ngtesteflp vsvvrkqskl
      121 sliftvsvav lvslnyinmg caldmevvkd vlrrpigpvv gffsqylamp lvaygmaqwl
      181 feeaylqlgl ftfgcspggg asnmwtvllg gnlnlsiamt fistiaslgt mplwlftlgr
      241 slfqeaslri pfrnifttli smtlplgvgl alqrwrpkva dvckrllapv svamivyivv
      301 fgtyanlymf qlmswqvlva aaanvwvgfa vgalatrcag lpwvdvlavs ietgvqntgi
      361 aivllgfslp qpeadiasvv pvaasivtpi pllllllvqk vysrccrrad tvadrkgvaa
      421 faevgaagda eeekraltvc ngalangtlg ggkaaeasyg avqqvg
//
LOCUS       XP_077503345             463 aa            linear   INV 25-NOV-2025
DEFINITION  ileal sodium/bile acid cotransporter-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503345
VERSION     XP_077503345.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647219.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..463
                     /product="ileal sodium/bile acid cotransporter-like
                     isoform X3"
                     /calculated_mol_wt=49160
     Region          120..405
                     /region_name="SBF"
                     /note="Sodium Bile acid symporter family; cl19217"
                     /db_xref="CDD:450274"
     CDS             1..463
                     /gene="LOC144113863"
                     /coded_by="XM_077647219.1:198..1589"
                     /db_xref="GeneID:144113863"
ORIGIN      
        1 mcpvvllall lsllllllev vaalsvafnp arvdklkqgs farvsfsapp leprffsvas
       61 dnpyvasvad lnftetgsfv ngtftvkanf lghakvrvvt ngtesteflp vsvvrkqskl
      121 sliftvsvav lvslnyinmg caldmevvkd vlrrpigpvv gffsqylamp lvaygmaqwl
      181 feeaylqlgl ftfgcspggg asnmwtvllg gnlnlsiamt fistiaslgt mplwlftlgr
      241 slfqeaslri pfrnifttli smtlplgvgl alqrwrpkva dvckrllapv svamivyivv
      301 fgtyanlymf qlmswqvlva aaanvwvgfa vgalatrcag lpwvdvlavs ietgvqntgi
      361 aivllgfslp qpeadiasvv pvaasivtpi pllllllvqk vysrccrrad tvadrkgvaa
      421 faevgaagda eeekraltvc ngalangtlg ggkaaeasyg tis
//
LOCUS       XP_077503346             443 aa            linear   INV 25-NOV-2025
DEFINITION  ileal sodium/bile acid cotransporter-like isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503346
VERSION     XP_077503346.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647220.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="ileal sodium/bile acid cotransporter-like
                     isoform X4"
                     /calculated_mol_wt=47336
     Region          120..405
                     /region_name="SBF"
                     /note="Sodium Bile acid symporter family; cl19217"
                     /db_xref="CDD:450274"
     CDS             1..443
                     /gene="LOC144113863"
                     /coded_by="XM_077647220.1:197..1528"
                     /db_xref="GeneID:144113863"
ORIGIN      
        1 mcpvvllall lsllllllev vaalsvafnp arvdklkqgs farvsfsapp leprffsvas
       61 dnpyvasvad lnftetgsfv ngtftvkanf lghakvrvvt ngtesteflp vsvvrkqskl
      121 sliftvsvav lvslnyinmg caldmevvkd vlrrpigpvv gffsqylamp lvaygmaqwl
      181 feeaylqlgl ftfgcspggg asnmwtvllg gnlnlsiamt fistiaslgt mplwlftlgr
      241 slfqeaslri pfrnifttli smtlplgvgl alqrwrpkva dvckrllapv svamivyivv
      301 fgtyanlymf qlmswqvlva aaanvwvgfa vgalatrcag lpwvdvlavs ietgvqntgi
      361 aivllgfslp qpeadiasvv pvaasivtpi pllllllvqk vysrccrrad tvadrkgvaa
      421 faegpsrdla alhvlavela agp
//
LOCUS       XP_077503347             436 aa            linear   INV 25-NOV-2025
DEFINITION  ileal sodium/bile acid cotransporter-like isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077503347
VERSION     XP_077503347.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647221.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..436
                     /product="ileal sodium/bile acid cotransporter-like
                     isoform X5"
                     /calculated_mol_wt=46885
     Region          120..405
                     /region_name="SBF"
                     /note="Sodium Bile acid symporter family; cl19217"
                     /db_xref="CDD:450274"
     CDS             1..436
                     /gene="LOC144113863"
                     /coded_by="XM_077647221.1:197..1507"
                     /db_xref="GeneID:144113863"
ORIGIN      
        1 mcpvvllall lsllllllev vaalsvafnp arvdklkqgs farvsfsapp leprffsvas
       61 dnpyvasvad lnftetgsfv ngtftvkanf lghakvrvvt ngtesteflp vsvvrkqskl
      121 sliftvsvav lvslnyinmg caldmevvkd vlrrpigpvv gffsqylamp lvaygmaqwl
      181 feeaylqlgl ftfgcspggg asnmwtvllg gnlnlsiamt fistiaslgt mplwlftlgr
      241 slfqeaslri pfrnifttli smtlplgvgl alqrwrpkva dvckrllapv svamivyivv
      301 fgtyanlymf qlmswqvlva aaanvwvgfa vgalatrcag lpwvdvlavs ietgvqntgi
      361 aivllgfslp qpeadiasvv pvaasivtpi pllllllvqk vysrccrrad tvadrkgvaa
      421 faevqynkld spllse
//
LOCUS       XP_077503348             226 aa            linear   INV 25-NOV-2025
DEFINITION  DUF3736 domain-containing protein Msr-110 [Amblyomma americanum].
ACCESSION   XP_077503348
VERSION     XP_077503348.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647222.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..226
                     /product="DUF3736 domain-containing protein Msr-110"
                     /calculated_mol_wt=23467
     CDS             1..226
                     /gene="Msr-110"
                     /coded_by="XM_077647222.1:104..784"
                     /db_xref="GeneID:144113864"
ORIGIN      
        1 mdkaqeaaaa aagmdypase lgsigrdllp ppayqrpgse rlkvsvvvas alvaislvvg
       61 vvvvlvvhlr aqqqlhcaca pppqaaalkt raggelssaa sgsvisklpm plklqldpla
      121 gqllkknqra qvscvvekkk aseviahepr tlmtpfgnmt teprmvhltg ermifscltg
      181 trdeprggst tpraplgata pgqgngpsrm krgiaeekdc acncsc
//
LOCUS       XP_077503349             328 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC144113865
            [Amblyomma americanum].
ACCESSION   XP_077503349
VERSION     XP_077503349.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647223.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts          :: corrected 1 indel
            internal stop codons :: corrected 1 genomic stop codon
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..328
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC144113865"
                     /calculated_mol_wt=36757
     CDS             1..328
                     /gene="LOC144113865"
                     /coded_by="XM_077647223.1:6442..7428"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 1 base in 1 codon;
                     substituted 1 base at 1 genomic stop codon"
                     /db_xref="GeneID:144113865"
ORIGIN      
        1 mvsdfpleym hlfclgamwr lfhywvqggp hkgkvparlq taisgmlmel qkyapqemar
       61 kprslceler wkatefrnfl fyfgpavlkn nieksyynhf mklsvalpil acpalcrsil
      121 rgfvvdaesl ygegsyvyni hslvhlptdv kkyghmdsfs afrlenslge lkqnlksanr
      181 plqqivkcly eqnqlaracp vrqaslhksh dsgptlprft geqfckitlp dmvfeintad
      241 scllmedgnv vvatnvlscd gdieicgiff tqlsnfhqgi agttxlhhyk asglxptnav
      301 wpvqqvrskc crfpcghgfv vmpllhtt
//
LOCUS       XP_077503350             160 aa            linear   INV 25-NOV-2025
DEFINITION  CXXC motif containing zinc binding protein [Amblyomma americanum].
ACCESSION   XP_077503350
VERSION     XP_077503350.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647224.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..160
                     /product="CXXC motif containing zinc binding protein"
                     /calculated_mol_wt=17632
     Region          6..156
                     /region_name="CXXC_Zn-b_euk"
                     /note="CXXC motif containing zinc binding protein,
                     eukaryotic; pfam05907"
                     /db_xref="CDD:461777"
     CDS             1..160
                     /gene="LOC144113867"
                     /coded_by="XM_077647224.1:327..809"
                     /db_xref="GeneID:144113867"
ORIGIN      
        1 mvkialqlra slenlagftp srdcvwrlkl kcmncgeets swqtveaanr tamrgsrgea
       61 nlvlkcklcc rensldvlnd rlltynaesa tnfatvavfe crgvepvsfd asdgfsaeat
      121 gsgtvfddvk fesnewaeyd ekgkqavgiy dltfrfskik
//
LOCUS       XP_077503351             283 aa            linear   INV 25-NOV-2025
DEFINITION  craniofacial development protein 1-like [Amblyomma americanum].
ACCESSION   XP_077503351
VERSION     XP_077503351.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647225.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..283
                     /product="craniofacial development protein 1-like"
                     /calculated_mol_wt=30869
     Region          207..276
                     /region_name="BCNT"
                     /note="Bucentaur or craniofacial development; pfam07572"
                     /db_xref="CDD:462213"
     CDS             1..283
                     /gene="LOC144113868"
                     /coded_by="XM_077647225.1:137..988"
                     /db_xref="GeneID:144113868"
ORIGIN      
        1 mstldlakpa dssesedddy vpsaeeseda seaencvgsd deesavkkrk kskkkpvsrk
       61 rkggihldge ddpvcnsags gaedgeketp aalteeeekk radqlwsdfl kdvepipkkr
      121 sapvtntvsa psksspsvtc pqaaapaekk vkvtqllefa getikvekev aadskeahlf
      181 akgqedvagq skspmlsgkr kpggvgnvln qlfnkkqkis tlekskldwd sykqsegiae
      241 dlqnhnrgkd gylekqaflq rtdlrqfeie ktlraksrst nyl
//
LOCUS       XP_077503352            1382 aa            linear   INV 25-NOV-2025
DEFINITION  capping protein regulator and myosin 1 linker 1 leucine rich repeat
            protein isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503352
VERSION     XP_077503352.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647226.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1382
                     /product="capping protein regulator and myosin 1 linker 1
                     leucine rich repeat protein isoform X1"
                     /calculated_mol_wt=151470
     Region          25..120
                     /region_name="Carm_PH"
                     /note="Carmil pleckstrin homology domain; pfam17888"
                     /db_xref="CDD:436119"
     Region          <211..388
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     Region          249..277
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          279..302
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          322..353
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <324..673
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     Region          354..380
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          381..406
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          442..467
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          470..499
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          500..526
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          562..588
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          800..1036
                     /region_name="CARMIL_C"
                     /note="CARMIL C-terminus; pfam16000"
                     /db_xref="CDD:464966"
     CDS             1..1382
                     /gene="LRR"
                     /coded_by="XM_077647226.1:159..4307"
                     /db_xref="GeneID:144113869"
ORIGIN      
        1 mstknaitke lsdsirhvld rsakvsfkcm vrlevkqdkt enrvlafssc rffiltakvp
       61 tkiehsfhyl eiqtveskkp nqlcltidnk vytfysvdpe ssdvdhmiiq lgtavksifp
      121 qvpleliirr veavpavrlq pmlefnqsae asdpgpcgnf saqyicmcdf hglpfredva
      181 wdvdtiylsh dtkelslldf dhlegrdlvp iisalcynaw ftkfrasnvk lasealeqvl
      241 qmmkrsvsle elyldntgik wefahklsmt lianpnspln sldlsnnlie dkglnslcgi
      301 iakitqgvgq lcgplgklhk glshlnlaht gitakgvntl ahalslnrsm psnltylnls
      361 dnvfkddinn lynylaqpns lvhldlsgte caletvfgal lrgctqklev lnlsrnvyss
      421 kkskevlvpp sfktffsstv alrhlnmanv rmpmealkam llglacneil tevwldlscn
      481 dlktqgalvl esclpgircl ysldisennf dqdlanivaa vgknqsikrl sigrnftnmk
      541 tkhitrvmea vvqliqekds sleylslads klrgelclvi nalgsnqclt siditgnymg
      601 lngaktlaka lqinsklksi lwdrnstpaq gfqdiayale knftlrympc pvvditlamk
      661 iapdrteaaw kkisellhrn vspkkhsnsq afrlqqgfll sstqqmvdrw mlhlqdvirt
      721 aqasssedvt cdeevlkaeg ylkdassakq lltrlhdvvl qqeeagnpie iklqgcvdel
      781 ektvnaylkg tasnmlkcie dqcptimsde kvrkeiedic rsrqhfpqev lqraiqdqag
      841 tdilnrisei nltiaahvsd rivdeviesl prshrdlvek lnrqrsstpd vlrsglrpds
      901 hsgdthcdqd svsessdspl atpklpikrk sincrklrpq svvdsvegia addipdllpk
      961 gsetdslpnl sptseklehl gkarpkrpkt haptrpclqa pesrepplse gldcffkrhp
     1021 ststptlspd sedaglksds stsasssili teskpleksp sdtkdtekrs fmkgisslfs
     1081 rasssssesv vvqkskqesk dtvssimsss msaytsvksr hdidvheken gemvtevrvs
     1141 ettrvsgmek fgvgpgsvil aemkafqgkr tsggapskgn vsedkgpgpi espkdtgpfp
     1201 avrlkptgla eslrspteyy preksctsps envpsspqls tpgsspktvt alksgrpppp
     1261 vapkprpksm vgtmesrlsg efsgdeclrt rartpdeadc ldssnksseg aswkratvys
     1321 kdspdaslas saastaanra affagsslrs sgmaassvvk ekaegelevm psappsddiv
     1381 dv
//
LOCUS       XP_077503353            1381 aa            linear   INV 25-NOV-2025
DEFINITION  capping protein regulator and myosin 1 linker 1 leucine rich repeat
            protein isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503353
VERSION     XP_077503353.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647227.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1381
                     /product="capping protein regulator and myosin 1 linker 1
                     leucine rich repeat protein isoform X2"
                     /calculated_mol_wt=151368
     Region          25..120
                     /region_name="Carm_PH"
                     /note="Carmil pleckstrin homology domain; pfam17888"
                     /db_xref="CDD:436119"
     Region          <211..388
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     Region          249..277
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          279..302
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          322..353
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <324..673
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     Region          354..380
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          381..406
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          442..467
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          470..499
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          500..526
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          562..588
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          800..1035
                     /region_name="CARMIL_C"
                     /note="CARMIL C-terminus; pfam16000"
                     /db_xref="CDD:464966"
     CDS             1..1381
                     /gene="LRR"
                     /coded_by="XM_077647227.1:159..4304"
                     /db_xref="GeneID:144113869"
ORIGIN      
        1 mstknaitke lsdsirhvld rsakvsfkcm vrlevkqdkt enrvlafssc rffiltakvp
       61 tkiehsfhyl eiqtveskkp nqlcltidnk vytfysvdpe ssdvdhmiiq lgtavksifp
      121 qvpleliirr veavpavrlq pmlefnqsae asdpgpcgnf saqyicmcdf hglpfredva
      181 wdvdtiylsh dtkelslldf dhlegrdlvp iisalcynaw ftkfrasnvk lasealeqvl
      241 qmmkrsvsle elyldntgik wefahklsmt lianpnspln sldlsnnlie dkglnslcgi
      301 iakitqgvgq lcgplgklhk glshlnlaht gitakgvntl ahalslnrsm psnltylnls
      361 dnvfkddinn lynylaqpns lvhldlsgte caletvfgal lrgctqklev lnlsrnvyss
      421 kkskevlvpp sfktffsstv alrhlnmanv rmpmealkam llglacneil tevwldlscn
      481 dlktqgalvl esclpgircl ysldisennf dqdlanivaa vgknqsikrl sigrnftnmk
      541 tkhitrvmea vvqliqekds sleylslads klrgelclvi nalgsnqclt siditgnymg
      601 lngaktlaka lqinsklksi lwdrnstpaq gfqdiayale knftlrympc pvvditlamk
      661 iapdrteaaw kkisellhrn vspkkhsnsq afrlqqgfll sstqqmvdrw mlhlqdvirt
      721 aqasssedvt cdeevlkaeg ylkdassakq lltrlhdvvl qqeeagnpie iklqgcvdel
      781 ektvnaylkg tasnmlkcie dqcptimsde kvrkeiedic rsrqhfpqev lqraiqdqag
      841 tdilnrisei nltiaahvsd rivdeviesl prshrdlvek lnrqrsstpd vlrsglrpds
      901 hsgdthcdqd svsessdspl atpklpikrk sincrklrpq svvdsvegia addipdllpk
      961 gsenslpnls ptseklehlg karpkrpkth aptrpclqap esrepplseg ldcffkrhps
     1021 tstptlspds edaglksdss tsasssilit eskpleksps dtkdtekrsf mkgisslfsr
     1081 asssssesvv vqkskqeskd tvssimsssm saytsvksrh didvhekeng emvtevrvse
     1141 ttrvsgmekf gvgpgsvila emkafqgkrt sggapskgnv sedkgpgpie spkdtgpfpa
     1201 vrlkptglae slrspteyyp reksctspse nvpsspqlst pgsspktvta lksgrppppv
     1261 apkprpksmv gtmesrlsge fsgdeclrtr artpdeadcl dssnkssega swkratvysk
     1321 dspdaslass aastaanraa ffagsslrss gmaassvvke kaegelevmp sappsddivd
     1381 v
//
LOCUS       XP_077503356            1368 aa            linear   INV 25-NOV-2025
DEFINITION  capping protein regulator and myosin 1 linker 1 leucine rich repeat
            protein isoform X3 [Amblyomma americanum].
ACCESSION   XP_077503356
VERSION     XP_077503356.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647230.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1368
                     /product="capping protein regulator and myosin 1 linker 1
                     leucine rich repeat protein isoform X3"
                     /calculated_mol_wt=150057
     Region          25..120
                     /region_name="Carm_PH"
                     /note="Carmil pleckstrin homology domain; pfam17888"
                     /db_xref="CDD:436119"
     Region          <234..493
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     Region          249..277
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Site            order(256,315,347,401,432,434,462,487)
                     /site_type="other"
                     /note="Substrate binding site [chemical binding]"
                     /db_xref="CDD:238064"
     Region          279..303
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          304..339
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <310..659
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     Region          340..366
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          367..392
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          394..427
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          428..453
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          456..485
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          486..512
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          548..574
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          786..1022
                     /region_name="CARMIL_C"
                     /note="CARMIL C-terminus; pfam16000"
                     /db_xref="CDD:464966"
     CDS             1..1368
                     /gene="LRR"
                     /coded_by="XM_077647230.1:164..4270"
                     /db_xref="GeneID:144113869"
ORIGIN      
        1 mstknaitke lsdsirhvld rsakvsfkcm vrlevkqdkt enrvlafssc rffiltakvp
       61 tkiehsfhyl eiqtveskkp nqlcltidnk vytfysvdpe ssdvdhmiiq lgtavksifp
      121 qvpleliirr veavpavrlq pmlefnqsae asdpgpcgnf saqyicmcdf hglpfredva
      181 wdvdtiylsh dtkelslldf dhlegrdlvp iisalcynaw ftkfrasnvk lasealeqvl
      241 qmmkrsvsle elyldntgik wefahklsmt lianpnspln sldlsnnlie dkgvgqlcgp
      301 lgklhkglsh lnlahtgita kgvntlahal slnrsmpsnl tylnlsdnvf kddinnlyny
      361 laqpnslvhl dlsgtecale tvfgallrgc tqklevlnls rnvysskksk evlvppsfkt
      421 ffsstvalrh lnmanvrmpm ealkamllgl acneiltevw ldlscndlkt qgalvlescl
      481 pgirclysld isennfdqdl anivaavgkn qsikrlsigr nftnmktkhi trvmeavvql
      541 iqekdssley lsladsklrg elclvinalg snqcltsidi tgnymglnga ktlakalqin
      601 sklksilwdr nstpaqgfqd iayaleknft lrympcpvvd itlamkiapd rteaawkkis
      661 ellhrnvspk khsnsqafrl qqgfllsstq qmvdrwmlhl qdvirtaqas ssedvtcdee
      721 vlkaegylkd assakqlltr lhdvvlqqee agnpieiklq gcvdelektv naylkgtasn
      781 mlkciedqcp timsdekvrk eiedicrsrq hfpqevlqra iqdqagtdil nriseinlti
      841 aahvsdrivd evieslprsh rdlveklnrq rsstpdvlrs glrpdshsgd thcdqdsvse
      901 ssdsplatpk lpikrksinc rklrpqsvvd svegiaaddi pdllpkgset dslpnlspts
      961 eklehlgkar pkrpkthapt rpclqapesr epplsegldc ffkrhpstst ptlspdseda
     1021 glksdsstsa sssilitesk plekspsdtk dtekrsfmkg isslfsrass sssesvvvqk
     1081 skqeskdtvs simsssmsay tsvksrhdid vhekengemv tevrvsettr vsgmekfgvg
     1141 pgsvilaemk afqgkrtsgg apskgnvsed kgpgpiespk dtgpfpavrl kptglaeslr
     1201 spteyyprek sctspsenvp sspqlstpgs spktvtalks grppppvapk prpksmvgtm
     1261 esrlsgefsg declrtrart pdeadcldss nkssegaswk ratvyskdsp daslassaas
     1321 taanraaffa gsslrssgma assvvkekae gelevmpsap psddivdv
//
LOCUS       XP_077503357            1367 aa            linear   INV 25-NOV-2025
DEFINITION  capping protein regulator and myosin 1 linker 1 leucine rich repeat
            protein isoform X4 [Amblyomma americanum].
ACCESSION   XP_077503357
VERSION     XP_077503357.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647231.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1367
                     /product="capping protein regulator and myosin 1 linker 1
                     leucine rich repeat protein isoform X4"
                     /calculated_mol_wt=149955
     Region          25..120
                     /region_name="Carm_PH"
                     /note="Carmil pleckstrin homology domain; pfam17888"
                     /db_xref="CDD:436119"
     Region          <234..493
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     Region          249..277
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Site            order(256,315,347,401,432,434,462,487)
                     /site_type="other"
                     /note="Substrate binding site [chemical binding]"
                     /db_xref="CDD:238064"
     Region          279..303
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          304..339
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <310..659
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     Region          340..366
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          367..392
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          394..427
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          428..453
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          456..485
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          486..512
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          548..574
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          786..1021
                     /region_name="CARMIL_C"
                     /note="CARMIL C-terminus; pfam16000"
                     /db_xref="CDD:464966"
     CDS             1..1367
                     /gene="LRR"
                     /coded_by="XM_077647231.1:159..4262"
                     /db_xref="GeneID:144113869"
ORIGIN      
        1 mstknaitke lsdsirhvld rsakvsfkcm vrlevkqdkt enrvlafssc rffiltakvp
       61 tkiehsfhyl eiqtveskkp nqlcltidnk vytfysvdpe ssdvdhmiiq lgtavksifp
      121 qvpleliirr veavpavrlq pmlefnqsae asdpgpcgnf saqyicmcdf hglpfredva
      181 wdvdtiylsh dtkelslldf dhlegrdlvp iisalcynaw ftkfrasnvk lasealeqvl
      241 qmmkrsvsle elyldntgik wefahklsmt lianpnspln sldlsnnlie dkgvgqlcgp
      301 lgklhkglsh lnlahtgita kgvntlahal slnrsmpsnl tylnlsdnvf kddinnlyny
      361 laqpnslvhl dlsgtecale tvfgallrgc tqklevlnls rnvysskksk evlvppsfkt
      421 ffsstvalrh lnmanvrmpm ealkamllgl acneiltevw ldlscndlkt qgalvlescl
      481 pgirclysld isennfdqdl anivaavgkn qsikrlsigr nftnmktkhi trvmeavvql
      541 iqekdssley lsladsklrg elclvinalg snqcltsidi tgnymglnga ktlakalqin
      601 sklksilwdr nstpaqgfqd iayaleknft lrympcpvvd itlamkiapd rteaawkkis
      661 ellhrnvspk khsnsqafrl qqgfllsstq qmvdrwmlhl qdvirtaqas ssedvtcdee
      721 vlkaegylkd assakqlltr lhdvvlqqee agnpieiklq gcvdelektv naylkgtasn
      781 mlkciedqcp timsdekvrk eiedicrsrq hfpqevlqra iqdqagtdil nriseinlti
      841 aahvsdrivd evieslprsh rdlveklnrq rsstpdvlrs glrpdshsgd thcdqdsvse
      901 ssdsplatpk lpikrksinc rklrpqsvvd svegiaaddi pdllpkgsen slpnlsptse
      961 klehlgkarp krpkthaptr pclqapesre pplsegldcf fkrhpststp tlspdsedag
     1021 lksdsstsas ssiliteskp lekspsdtkd tekrsfmkgi sslfsrasss ssesvvvqks
     1081 kqeskdtvss imsssmsayt svksrhdidv hekengemvt evrvsettrv sgmekfgvgp
     1141 gsvilaemka fqgkrtsgga pskgnvsedk gpgpiespkd tgpfpavrlk ptglaeslrs
     1201 pteyypreks ctspsenvps spqlstpgss pktvtalksg rppppvapkp rpksmvgtme
     1261 srlsgefsgd eclrtrartp deadcldssn kssegaswkr atvyskdspd aslassaast
     1321 aanraaffag sslrssgmaa ssvvkekaeg elevmpsapp sddivdv
//
LOCUS       XP_077503358            1362 aa            linear   INV 25-NOV-2025
DEFINITION  capping protein regulator and myosin 1 linker 1 leucine rich repeat
            protein isoform X5 [Amblyomma americanum].
ACCESSION   XP_077503358
VERSION     XP_077503358.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647232.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1362
                     /product="capping protein regulator and myosin 1 linker 1
                     leucine rich repeat protein isoform X5"
                     /calculated_mol_wt=149417
     Region          25..120
                     /region_name="Carm_PH"
                     /note="Carmil pleckstrin homology domain; pfam17888"
                     /db_xref="CDD:436119"
     Region          <211..388
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     Region          249..277
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          279..302
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          322..353
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <324..673
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     Region          354..380
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          381..406
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          442..467
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          470..499
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          500..526
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          562..588
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          800..1016
                     /region_name="CARMIL_C"
                     /note="CARMIL C-terminus; pfam16000"
                     /db_xref="CDD:464966"
     CDS             1..1362
                     /gene="LRR"
                     /coded_by="XM_077647232.1:159..4247"
                     /db_xref="GeneID:144113869"
ORIGIN      
        1 mstknaitke lsdsirhvld rsakvsfkcm vrlevkqdkt enrvlafssc rffiltakvp
       61 tkiehsfhyl eiqtveskkp nqlcltidnk vytfysvdpe ssdvdhmiiq lgtavksifp
      121 qvpleliirr veavpavrlq pmlefnqsae asdpgpcgnf saqyicmcdf hglpfredva
      181 wdvdtiylsh dtkelslldf dhlegrdlvp iisalcynaw ftkfrasnvk lasealeqvl
      241 qmmkrsvsle elyldntgik wefahklsmt lianpnspln sldlsnnlie dkglnslcgi
      301 iakitqgvgq lcgplgklhk glshlnlaht gitakgvntl ahalslnrsm psnltylnls
      361 dnvfkddinn lynylaqpns lvhldlsgte caletvfgal lrgctqklev lnlsrnvyss
      421 kkskevlvpp sfktffsstv alrhlnmanv rmpmealkam llglacneil tevwldlscn
      481 dlktqgalvl esclpgircl ysldisennf dqdlanivaa vgknqsikrl sigrnftnmk
      541 tkhitrvmea vvqliqekds sleylslads klrgelclvi nalgsnqclt siditgnymg
      601 lngaktlaka lqinsklksi lwdrnstpaq gfqdiayale knftlrympc pvvditlamk
      661 iapdrteaaw kkisellhrn vspkkhsnsq afrlqqgfll sstqqmvdrw mlhlqdvirt
      721 aqasssedvt cdeevlkaeg ylkdassakq lltrlhdvvl qqeeagnpie iklqgcvdel
      781 ektvnaylkg tasnmlkcie dqcptimsde kvrkeiedic rsrqhfpqev lqraiqdqag
      841 tdilnrisei nltiaahvsd rivdeviesl prshrdlvek lnrqrsstpd vlrsglrpds
      901 hsgdthcdqd svsessdspl atpklpikrk sincrklrpq svvadslpnl sptseklehl
      961 gkarpkrpkt haptrpclqa pesrepplse gldcffkrhp ststptlspd sedaglksds
     1021 stsasssili teskpleksp sdtkdtekrs fmkgisslfs rasssssesv vvqkskqesk
     1081 dtvssimsss msaytsvksr hdidvheken gemvtevrvs ettrvsgmek fgvgpgsvil
     1141 aemkafqgkr tsggapskgn vsedkgpgpi espkdtgpfp avrlkptgla eslrspteyy
     1201 preksctsps envpsspqls tpgsspktvt alksgrpppp vapkprpksm vgtmesrlsg
     1261 efsgdeclrt rartpdeadc ldssnksseg aswkratvys kdspdaslas saastaanra
     1321 affagsslrs sgmaassvvk ekaegelevm psappsddiv dv
//
LOCUS       XP_077503359            1361 aa            linear   INV 25-NOV-2025
DEFINITION  capping protein regulator and myosin 1 linker 1 leucine rich repeat
            protein isoform X6 [Amblyomma americanum].
ACCESSION   XP_077503359
VERSION     XP_077503359.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647233.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1361
                     /product="capping protein regulator and myosin 1 linker 1
                     leucine rich repeat protein isoform X6"
                     /calculated_mol_wt=149346
     Region          25..120
                     /region_name="Carm_PH"
                     /note="Carmil pleckstrin homology domain; pfam17888"
                     /db_xref="CDD:436119"
     Region          <211..388
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     Region          249..277
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          279..302
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          322..353
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <324..673
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     Region          354..380
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          381..406
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          442..467
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          470..499
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          500..526
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          562..588
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          800..1015
                     /region_name="CARMIL_C"
                     /note="CARMIL C-terminus; pfam16000"
                     /db_xref="CDD:464966"
     CDS             1..1361
                     /gene="LRR"
                     /coded_by="XM_077647233.1:159..4244"
                     /db_xref="GeneID:144113869"
ORIGIN      
        1 mstknaitke lsdsirhvld rsakvsfkcm vrlevkqdkt enrvlafssc rffiltakvp
       61 tkiehsfhyl eiqtveskkp nqlcltidnk vytfysvdpe ssdvdhmiiq lgtavksifp
      121 qvpleliirr veavpavrlq pmlefnqsae asdpgpcgnf saqyicmcdf hglpfredva
      181 wdvdtiylsh dtkelslldf dhlegrdlvp iisalcynaw ftkfrasnvk lasealeqvl
      241 qmmkrsvsle elyldntgik wefahklsmt lianpnspln sldlsnnlie dkglnslcgi
      301 iakitqgvgq lcgplgklhk glshlnlaht gitakgvntl ahalslnrsm psnltylnls
      361 dnvfkddinn lynylaqpns lvhldlsgte caletvfgal lrgctqklev lnlsrnvyss
      421 kkskevlvpp sfktffsstv alrhlnmanv rmpmealkam llglacneil tevwldlscn
      481 dlktqgalvl esclpgircl ysldisennf dqdlanivaa vgknqsikrl sigrnftnmk
      541 tkhitrvmea vvqliqekds sleylslads klrgelclvi nalgsnqclt siditgnymg
      601 lngaktlaka lqinsklksi lwdrnstpaq gfqdiayale knftlrympc pvvditlamk
      661 iapdrteaaw kkisellhrn vspkkhsnsq afrlqqgfll sstqqmvdrw mlhlqdvirt
      721 aqasssedvt cdeevlkaeg ylkdassakq lltrlhdvvl qqeeagnpie iklqgcvdel
      781 ektvnaylkg tasnmlkcie dqcptimsde kvrkeiedic rsrqhfpqev lqraiqdqag
      841 tdilnrisei nltiaahvsd rivdeviesl prshrdlvek lnrqrsstpd vlrsglrpds
      901 hsgdthcdqd svsessdspl atpklpikrk sincrklrpq svvdslpnls ptseklehlg
      961 karpkrpkth aptrpclqap esrepplseg ldcffkrhps tstptlspds edaglksdss
     1021 tsasssilit eskpleksps dtkdtekrsf mkgisslfsr asssssesvv vqkskqeskd
     1081 tvssimsssm saytsvksrh didvhekeng emvtevrvse ttrvsgmekf gvgpgsvila
     1141 emkafqgkrt sggapskgnv sedkgpgpie spkdtgpfpa vrlkptglae slrspteyyp
     1201 reksctspse nvpsspqlst pgsspktvta lksgrppppv apkprpksmv gtmesrlsge
     1261 fsgdeclrtr artpdeadcl dssnkssega swkratvysk dspdaslass aastaanraa
     1321 ffagsslrss gmaassvvke kaegelevmp sappsddivd v
//
LOCUS       XP_077503360            1348 aa            linear   INV 25-NOV-2025
DEFINITION  capping protein regulator and myosin 1 linker 1 leucine rich repeat
            protein isoform X7 [Amblyomma americanum].
ACCESSION   XP_077503360
VERSION     XP_077503360.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647234.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1348
                     /product="capping protein regulator and myosin 1 linker 1
                     leucine rich repeat protein isoform X7"
                     /calculated_mol_wt=148004
     Region          25..120
                     /region_name="Carm_PH"
                     /note="Carmil pleckstrin homology domain; pfam17888"
                     /db_xref="CDD:436119"
     Region          <234..493
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     Region          249..277
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Site            order(256,315,347,401,432,434,462,487)
                     /site_type="other"
                     /note="Substrate binding site [chemical binding]"
                     /db_xref="CDD:238064"
     Region          279..303
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          304..339
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <310..659
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     Region          340..366
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          367..392
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          394..427
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          428..453
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          456..485
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          486..512
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          548..574
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          786..1002
                     /region_name="CARMIL_C"
                     /note="CARMIL C-terminus; pfam16000"
                     /db_xref="CDD:464966"
     CDS             1..1348
                     /gene="LRR"
                     /coded_by="XM_077647234.1:159..4205"
                     /db_xref="GeneID:144113869"
ORIGIN      
        1 mstknaitke lsdsirhvld rsakvsfkcm vrlevkqdkt enrvlafssc rffiltakvp
       61 tkiehsfhyl eiqtveskkp nqlcltidnk vytfysvdpe ssdvdhmiiq lgtavksifp
      121 qvpleliirr veavpavrlq pmlefnqsae asdpgpcgnf saqyicmcdf hglpfredva
      181 wdvdtiylsh dtkelslldf dhlegrdlvp iisalcynaw ftkfrasnvk lasealeqvl
      241 qmmkrsvsle elyldntgik wefahklsmt lianpnspln sldlsnnlie dkgvgqlcgp
      301 lgklhkglsh lnlahtgita kgvntlahal slnrsmpsnl tylnlsdnvf kddinnlyny
      361 laqpnslvhl dlsgtecale tvfgallrgc tqklevlnls rnvysskksk evlvppsfkt
      421 ffsstvalrh lnmanvrmpm ealkamllgl acneiltevw ldlscndlkt qgalvlescl
      481 pgirclysld isennfdqdl anivaavgkn qsikrlsigr nftnmktkhi trvmeavvql
      541 iqekdssley lsladsklrg elclvinalg snqcltsidi tgnymglnga ktlakalqin
      601 sklksilwdr nstpaqgfqd iayaleknft lrympcpvvd itlamkiapd rteaawkkis
      661 ellhrnvspk khsnsqafrl qqgfllsstq qmvdrwmlhl qdvirtaqas ssedvtcdee
      721 vlkaegylkd assakqlltr lhdvvlqqee agnpieiklq gcvdelektv naylkgtasn
      781 mlkciedqcp timsdekvrk eiedicrsrq hfpqevlqra iqdqagtdil nriseinlti
      841 aahvsdrivd evieslprsh rdlveklnrq rsstpdvlrs glrpdshsgd thcdqdsvse
      901 ssdsplatpk lpikrksinc rklrpqsvva dslpnlspts eklehlgkar pkrpkthapt
      961 rpclqapesr epplsegldc ffkrhpstst ptlspdseda glksdsstsa sssilitesk
     1021 plekspsdtk dtekrsfmkg isslfsrass sssesvvvqk skqeskdtvs simsssmsay
     1081 tsvksrhdid vhekengemv tevrvsettr vsgmekfgvg pgsvilaemk afqgkrtsgg
     1141 apskgnvsed kgpgpiespk dtgpfpavrl kptglaeslr spteyyprek sctspsenvp
     1201 sspqlstpgs spktvtalks grppppvapk prpksmvgtm esrlsgefsg declrtrart
     1261 pdeadcldss nkssegaswk ratvyskdsp daslassaas taanraaffa gsslrssgma
     1321 assvvkekae gelevmpsap psddivdv
//
LOCUS       XP_077503361             669 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113870 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503361
VERSION     XP_077503361.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647235.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..669
                     /product="uncharacterized protein LOC144113870 isoform X1"
                     /calculated_mol_wt=71967
     Region          <80..350
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..669
                     /gene="LOC144113870"
                     /coded_by="XM_077647235.1:154..2163"
                     /db_xref="GeneID:144113870"
ORIGIN      
        1 msewdfdcpk yvslprasdd egdsspesyf edrkedgvfr rmtrsmtaal gtvavdaaqe
       61 alssaavkar sdasavstpa kattsplken chpeaekpth kqrverlgkr rplrvcnvdg
      121 srcrptlckp applstkgpa tvltvkkppt asagrkppta sagrkpptas aarkpptasa
      181 arkpptasaa rkpptvsaar kpptasaark pptasagrkp ptasagrkpp tastgrkppt
      241 asagrkppia saarkpptas aarkpptasa arkpptasaa rkpptasaar kpptasmakk
      301 pptasmakkp ptastakkpp tastakkppt lltmkkpaaa sreksatlsa arkpaassei
      361 rkpgrapaam cqslaelvgn fhtktptrfr kvfkaeggqc rnmeprkpti pvtpklatrl
      421 rsravrardq dehvsayssk pkrpkltdaq vkpwvavwpk sdpasstvkp ecgslvskpl
      481 mevlstksvk tpceklkkvl ritvpkspaf alkvrsetwk qrkeknvldl avcqrtavlh
      541 rrnlsqqfld rqkrtlqhke patgaavavk qvepqkfral papdpkrvfr prnlsvpatk
      601 prpfrlasve rhekcqeefr rktkqleaer vkmaqfvakp vpaflkarrn sineakpgtl
      661 ghvsrprrr
//
LOCUS       XP_077503362             668 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113870 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503362
VERSION     XP_077503362.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647236.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..668
                     /product="uncharacterized protein LOC144113870 isoform X2"
                     /calculated_mol_wt=71838
     Region          <79..349
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..668
                     /gene="LOC144113870"
                     /coded_by="XM_077647236.1:141..2147"
                     /db_xref="GeneID:144113870"
ORIGIN      
        1 msewdfdcpk yvslprasdd egdsspesyf drkedgvfrr mtrsmtaalg tvavdaaqea
       61 lssaavkars dasavstpak attsplkenc hpeaekpthk qrverlgkrr plrvcnvdgs
      121 rcrptlckpa pplstkgpat vltvkkppta sagrkpptas agrkpptasa arkpptasaa
      181 rkpptasaar kpptvsaark pptasaarkp ptasagrkpp tasagrkppt astgrkppta
      241 sagrkppias aarkpptasa arkpptasaa rkpptasaar kpptasaark pptasmakkp
      301 ptasmakkpp tastakkppt astakkpptl ltmkkpaaas reksatlsaa rkpaasseir
      361 kpgrapaamc qslaelvgnf htktptrfrk vfkaeggqcr nmeprkptip vtpklatrlr
      421 sravrardqd ehvsaysskp krpkltdaqv kpwvavwpks dpasstvkpe cgslvskplm
      481 evlstksvkt pceklkkvlr itvpkspafa lkvrsetwkq rkeknvldla vcqrtavlhr
      541 rnlsqqfldr qkrtlqhkep atgaavavkq vepqkfralp apdpkrvfrp rnlsvpatkp
      601 rpfrlasver hekcqeefrr ktkqleaerv kmaqfvakpv paflkarrns ineakpgtlg
      661 hvsrprrr
//
LOCUS       XP_077503363             668 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113870 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503363
VERSION     XP_077503363.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647237.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..668
                     /product="uncharacterized protein LOC144113870 isoform X3"
                     /calculated_mol_wt=71811
     Region          <79..349
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..668
                     /gene="LOC144113870"
                     /coded_by="XM_077647237.1:141..2147"
                     /db_xref="GeneID:144113870"
ORIGIN      
        1 msewdfdcpk yvslprasdd egdsspesyf edrkedgvfr mtrsmtaalg tvavdaaqea
       61 lssaavkars dasavstpak attsplkenc hpeaekpthk qrverlgkrr plrvcnvdgs
      121 rcrptlckpa pplstkgpat vltvkkppta sagrkpptas agrkpptasa arkpptasaa
      181 rkpptasaar kpptvsaark pptasaarkp ptasagrkpp tasagrkppt astgrkppta
      241 sagrkppias aarkpptasa arkpptasaa rkpptasaar kpptasaark pptasmakkp
      301 ptasmakkpp tastakkppt astakkpptl ltmkkpaaas reksatlsaa rkpaasseir
      361 kpgrapaamc qslaelvgnf htktptrfrk vfkaeggqcr nmeprkptip vtpklatrlr
      421 sravrardqd ehvsaysskp krpkltdaqv kpwvavwpks dpasstvkpe cgslvskplm
      481 evlstksvkt pceklkkvlr itvpkspafa lkvrsetwkq rkeknvldla vcqrtavlhr
      541 rnlsqqfldr qkrtlqhkep atgaavavkq vepqkfralp apdpkrvfrp rnlsvpatkp
      601 rpfrlasver hekcqeefrr ktkqleaerv kmaqfvakpv paflkarrns ineakpgtlg
      661 hvsrprrr
//
LOCUS       XP_077503364             667 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113870 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503364
VERSION     XP_077503364.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647238.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..667
                     /product="uncharacterized protein LOC144113870 isoform X4"
                     /calculated_mol_wt=71681
     Region          <78..348
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..667
                     /gene="LOC144113870"
                     /coded_by="XM_077647238.1:141..2144"
                     /db_xref="GeneID:144113870"
ORIGIN      
        1 msewdfdcpk yvslprasdd egdsspesyf drkedgvfrm trsmtaalgt vavdaaqeal
       61 ssaavkarsd asavstpaka ttsplkench peaekpthkq rverlgkrrp lrvcnvdgsr
      121 crptlckpap plstkgpatv ltvkkpptas agrkpptasa grkpptasaa rkpptasaar
      181 kpptasaark pptvsaarkp ptasaarkpp tasagrkppt asagrkppta stgrkpptas
      241 agrkppiasa arkpptasaa rkpptasaar kpptasaark pptasaarkp ptasmakkpp
      301 tasmakkppt astakkppta stakkpptll tmkkpaaasr eksatlsaar kpaasseirk
      361 pgrapaamcq slaelvgnfh tktptrfrkv fkaeggqcrn meprkptipv tpklatrlrs
      421 ravrardqde hvsaysskpk rpkltdaqvk pwvavwpksd passtvkpec gslvskplme
      481 vlstksvktp ceklkkvlri tvpkspafal kvrsetwkqr keknvldlav cqrtavlhrr
      541 nlsqqfldrq krtlqhkepa tgaavavkqv epqkfralpa pdpkrvfrpr nlsvpatkpr
      601 pfrlasverh ekcqeefrrk tkqleaervk maqfvakpvp aflkarrnsi neakpgtlgh
      661 vsrprrr
//
LOCUS       XP_077503365             628 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113870 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077503365
VERSION     XP_077503365.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647239.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..628
                     /product="uncharacterized protein LOC144113870 isoform X5"
                     /calculated_mol_wt=67329
     Region          <80..350
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..628
                     /gene="LOC144113870"
                     /coded_by="XM_077647239.1:180..2066"
                     /db_xref="GeneID:144113870"
ORIGIN      
        1 msewdfdcpk yvslprasdd egdsspesyf edrkedgvfr rmtrsmtaal gtvavdaaqe
       61 alssaavkar sdasavstpa kattsplken chpeaekpth kqrverlgkr rplrvcnvdg
      121 srcrptlckp applstkgpa tvltvkkppt asagrkppta sagrkpptas aarkpptasa
      181 arkpptasaa rkpptvsaar kpptasaark pptasagrkp ptasagrkpp tastgrkppt
      241 asagrkppia saarkpptas aarkpptasa arkpptasaa rkpptasaar kpptasmakk
      301 pptasmakkp ptastakkpp tastakkppt lltmkkpaaa sreksatlsa arkpaassei
      361 rkpgrapaam cqslaelvgn fhtktptrfr kvfkaeggqc rnmeprkpti pvtpklatrl
      421 rsravrardq dehvsayssk pkrpkltdaq vkpwvavwpk sdpasstvkp ecgslvskpl
      481 mevlstksvk tpceklkkvl ritvpkspaf alkvrsetwk qrkeknvldl vepqkfralp
      541 apdpkrvfrp rnlsvpatkp rpfrlasver hekcqeefrr ktkqleaerv kmaqfvakpv
      601 paflkarrns ineakpgtlg hvsrprrr
//
LOCUS       XP_077503366             591 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113870 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077503366
VERSION     XP_077503366.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647240.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..591
                     /product="uncharacterized protein LOC144113870 isoform X6"
                     /calculated_mol_wt=62969
     Region          <124..342
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..591
                     /gene="LOC144113870"
                     /coded_by="XM_077647240.1:154..1929"
                     /db_xref="GeneID:144113870"
ORIGIN      
        1 msewdfdcpk yvslprasdd egdsspesyf edrkedgvfr rmtrsmtaal gtvavdaaqe
       61 alssaavkar sdasavstpa kattsplken chpeaekpth kqrverlgkr rplrvcnvdg
      121 srcrptlckp applstkgpa tvltvkkppt asagrkppta sagrkpptas aarkpptasa
      181 arkpptasaa rkpptvsaar kpptasaark pptasagrkp ptasagrkpp tastgrkppt
      241 asagrkppia saarkpptas aarkpptasa arkpptasaa rkpptasaar kpptasmakk
      301 pptasmakkp ptastakkpp tastakkppt lltmkkpaaa sreksatlsa arkpaassei
      361 rkpgrapaam cqslaelvgn fhtktptrfr kvfkaeggqc rnmeprkpti pvtpklatrl
      421 rsravrardq dehvsayssk pkrpkltdaq vkpwvavwpk sdpasstvkp ecgslvskpl
      481 mevlstksvk tpcvepqkfr alpapdpkrv frprnlsvpa tkprpfrlas verhekcqee
      541 frrktkqlea ervkmaqfva kpvpaflkar rnsineakpg tlghvsrprr r
//
LOCUS       XP_077503367             580 aa            linear   INV 25-NOV-2025
DEFINITION  glomulin [Amblyomma americanum].
ACCESSION   XP_077503367
VERSION     XP_077503367.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647241.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..580
                     /product="glomulin"
                     /calculated_mol_wt=64888
     Region          <349..526
                     /region_name="Kinetochor_Ybp2"
                     /note="Uncharacterized protein family, YAP/Alf4/glomulin;
                     pfam08568"
                     /db_xref="CDD:400745"
     CDS             1..580
                     /gene="LOC144113872"
                     /coded_by="XM_077647241.1:134..1876"
                     /db_xref="GeneID:144113872"
ORIGIN      
        1 meemdcvdpi araveflnsq kyneakafls evapdvifth gwdlvlpvlt amekeidakc
       61 dvnvclckel levigsrgkp kevlislleh lqlvkssagl havlqcfqkl lqgplgnklk
      121 fldmildsly ihlkrlsasv aknsgeqtsd dvstetpesq nlgcclnama dffsevtrgc
      181 dlqkesrlve dprtenelfl kylmlflgev gvldlqrhpd tspnfahlsa erlvshissr
      241 stdliklcis geetrqckek ssdaedtekd lpepvspicr avlawsifve nfeqdnvpcv
      301 yasryvfeyn lqnimillth ssghvvvkgv alcanmvsrl nhgefssldl dnsnlmsllr
      361 clgkvmiysc sskarkaals vlvdyikcfk yearyilycr mlqvekhagl hgllidlyrq
      421 dmhatlkrsn qddtflgvnf veflkivvsn fssqnltdil ddsdsvlavl nllryfafvm
      481 phqgqicaka tqsvceiaen ytkqvkksld qtriqykael ehskeevksg knpkdslfps
      541 lppeqeadvl ktaltrldlv esvlvlavds lghcanqllq
//
LOCUS       XP_077503368             429 aa            linear   INV 25-NOV-2025
DEFINITION  GDP-mannose pyrophosphorylase A [Amblyomma americanum].
ACCESSION   XP_077503368
VERSION     XP_077503368.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647242.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..429
                     /product="GDP-mannose pyrophosphorylase A"
                     /calculated_mol_wt=46354
     Region          16..269
                     /region_name="M1P_guanylylT_A_like_N"
                     /note="N-terminal domain of M1P_guanylyl_A_ like proteins
                     are likely to be a isoform of GDP-mannose
                     pyrophosphorylase; cd06428"
                     /db_xref="CDD:133050"
     Site            order(19..22,38,127..129,195)
                     /site_type="active"
                     /note="putative substrate binding site [active]"
                     /db_xref="CDD:133050"
     Site            order(129,255,257)
                     /site_type="active"
                     /note="metal binding site [active]"
                     /db_xref="CDD:133050"
     Region          297..363
                     /region_name="LbetaH"
                     /note="Left-handed parallel beta-Helix (LbetaH or LbH)
                     domain: The alignment contains 5 turns, each containing
                     three imperfect tandem repeats of a hexapeptide repeat
                     motif (X-[STAV]-X-[LIV]-[GAED]-X). Proteins containing
                     hexapeptide repeats are often enzymes...; cl00160"
                     /db_xref="CDD:469633"
     Site            order(308,310,314,316,326,331,333,343,347..348,350,360)
                     /site_type="other"
                     /note="putative trimer interface [polypeptide binding]"
                     /db_xref="CDD:100062"
     Site            order(343,345..346,350,362)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:100062"
     CDS             1..429
                     /gene="Gmppa"
                     /coded_by="XM_077647242.1:250..1539"
                     /db_xref="GeneID:144113873"
ORIGIN      
        1 mpasptpprt agmlkavili ggpqkgtrfr plsfdipkpl fpvagrpmmq hlveaccglp
       61 gvreilligf ypaddadltg fvksasreyg lpvrylqeya algtaggihh frdqirrgdp
      121 eafllihgdv cghfplaeml afhrslpksn lvtvlateat rqqslsygci vedktthqvl
      181 hyvekpstfv savincgvyl cspdvfqrtg aalrdrhale gdhldalsle hdvlaplaaa
      241 grqlhvfqts hwwsqlktag saiyanrhyl elyrlehper larpgpegpt ilgdvfvhps
      301 asvdptatlg pnvsvgpgar igagvrires lvlanatvcd halvlhsivg ldssvgawsr
      361 vegtpcdpnp drpfakmenv plfnadgrln psitvlgchv svppevivln sivlphkdla
      421 qsykneiil
//
LOCUS       XP_077503370             278 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquinone biosynthesis protein COQ9, mitochondrial [Amblyomma
            americanum].
ACCESSION   XP_077503370
VERSION     XP_077503370.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647244.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..278
                     /product="ubiquinone biosynthesis protein COQ9,
                     mitochondrial"
                     /calculated_mol_wt=30334
     Region          62..246
                     /region_name="COQ9"
                     /note="cl21709"
                     /db_xref="CDD:473940"
     CDS             1..278
                     /gene="Coq9"
                     /coded_by="XM_077647244.1:71..907"
                     /db_xref="GeneID:144113874"
ORIGIN      
        1 maallarkvv lenyvakpav vwrrglradh ircvakkdtt lggqeqkagi sdlisgqagd
       61 vknsilrsal dfipqhgwtl ksiaagassl glssvahglf pggsldlity fylssndele
      121 hqlagiaqks drravtkeks afiqdavwkr llllepykdt wvqalgilal pqnvpvavsn
      181 lgdllniiwy hagdmstdms wyskravlaa lyqsselvfl qdksfdnaqt qeflnrrike
      241 laafsscveq vkqatgtakd vaelgfstik nvlgintw
//
LOCUS       XP_077503371             241 aa            linear   INV 25-NOV-2025
DEFINITION  deoxynucleoside kinase [Amblyomma americanum].
ACCESSION   XP_077503371
VERSION     XP_077503371.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647245.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..241
                     /product="deoxynucleoside kinase"
                     /calculated_mol_wt=27544
     Region          21..206
                     /region_name="dNK"
                     /note="Deoxyribonucleoside kinase (dNK) catalyzes the
                     phosphorylation of deoxyribonucleosides to yield
                     corresponding monophosphates (dNMPs). This family consists
                     of various deoxynucleoside kinases including deoxyribo-
                     cytidine (EC 2.7.1.74), guanosine (EC 2.7.1; cd01673"
                     /db_xref="CDD:238836"
     Site            order(29,31..32,67,77..78,111,164,169)
                     /site_type="other"
                     /note="Substrate-binding site [chemical binding]"
                     /db_xref="CDD:238836"
     Site            85
                     /site_type="other"
                     /note="Substrate specificity [chemical binding]"
                     /db_xref="CDD:238836"
     CDS             1..241
                     /gene="dnk"
                     /coded_by="XM_077647245.1:121..846"
                     /db_xref="GeneID:144113875"
ORIGIN      
        1 mnvvgdaqlq kaarkgrtvn vavegnigcg kttflegckq fldtsvliep vniwrdiqgh
       61 nllelmyrdp krwslafqty vqltmmqlhl apvqtpvklm erslqsaryv fvenlhrsgh
      121 mtsvelsild qwfswitkne avgldmiiyl rtnpqvamsr ilerkrpeea dfpfdwlchv
      181 helheqwllg rsqfplppkv mvvdankdct diqqefaqhl pdildrsqlc haplangpss
      241 n
//
LOCUS       XP_077503372             430 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113876 [Amblyomma americanum].
ACCESSION   XP_077503372
VERSION     XP_077503372.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647246.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..430
                     /product="uncharacterized protein LOC144113876"
                     /calculated_mol_wt=47134
     Region          268..367
                     /region_name="SH2_SOCS6"
                     /note="Src homology 2 (SH2) domain found in suppressor of
                     cytokine signaling (SOCS) proteins; cd10387"
                     /db_xref="CDD:198250"
     Site            order(286,304,306,314,327)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198250"
     Site            order(337,339,356)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198250"
     Region          390..430
                     /region_name="SOCS_SOCS6"
                     /note="SOCS (suppressors of cytokine signaling) box of
                     SOCS6-like proteins. Together with CIS1, the CIS/SOCS
                     family of proteins is characterized by the presence of a
                     C-terminal SOCS box and a central SH2 domain. The general
                     function of the SOCS box is the...; cd03740"
                     /db_xref="CDD:239709"
     Site            order(391..396,402,409,415,420)
                     /site_type="other"
                     /note="putative elongin B/C interaction [polypeptide
                     binding]"
                     /db_xref="CDD:239709"
     CDS             1..430
                     /gene="LOC144113876"
                     /coded_by="XM_077647246.1:254..1546"
                     /db_xref="GeneID:144113876"
ORIGIN      
        1 mllrrkfpsv sgtrgqtnan gdmaqektes dledcqrlst tgksklksnf fkslrehwrk
       61 rktklgvqsg tekcpvvpes strirgvtvy rpgssslaap eshqgarnda ndnyvrarvg
      121 sngesacssv daeseaprrs mpasvregqp epvggttehs dhgqpsseqr rtacsmqppa
      181 tnsnwclshp tsgfshscss sapevqwkct gphtdgttqp aagagyseas aaaegpqvpp
      241 rpalrgatas vfapgmdgre dprisslsee lfklskhgwy wgpitrseae ekladqpdga
      301 flvrdssddr yllsltfksy gktlhtrieh cnglfsfyaq sgpegraciv dliqesmnys
      361 qsgvfcysrg rspgvpsfpv rlikpvsrlt hvrslqylcr fvirqytrfd hiqrlplpsr
      421 ikgyleeghy
//
LOCUS       XP_077503373             875 aa            linear   INV 25-NOV-2025
DEFINITION  disc proliferation abnormal isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503373
VERSION     XP_077503373.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647247.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..875
                     /product="disc proliferation abnormal isoform X1"
                     /calculated_mol_wt=97761
     Region          173..275
                     /region_name="MCM_N"
                     /note="MCM N-terminal domain; pfam14551"
                     /db_xref="CDD:464206"
     Region          278..781
                     /region_name="MCM"
                     /note="minichromosome maintenance proteins; smart00350"
                     /db_xref="CDD:214631"
     Site            order(479,501,523..524,529,532,536,543..545,547..548,
                     550..551,553,557,562,569..571,575,578,586,589,596,
                     599..600,604,608,610..612,614..617,619,629,633..634,650,
                     663,670..671,674,678..679,681..682,713,718,721..722,725,
                     729,741..742,745..746,749)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:350661"
     Site            order(480..482,523..529,603,654,673,677,742..743)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350661"
     Site            order(550,552,557,562,611..612)
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:350661"
     CDS             1..875
                     /gene="dpa"
                     /coded_by="XM_077647247.1:169..2796"
                     /db_xref="GeneID:144113878"
ORIGIN      
        1 msspsrtprr ntrsasrepr tpsrglrstp srkntparst rgtpsrrqrd gtgtdggaag
       61 vsaapsssid gvnevppssp yqrnlpvfte ldlssplhyg tpssatgtpr snargtpirh
      121 rtdirndrrm rqvaigasdg dtgeqtgava tsdsgtgpql viwgtdvvvs hckekfkqfl
      181 rtfaevnlat delmdgvdpv qplylqklee lymleepfln vncqhvanfd adlyrqlkcy
      241 pqeviptfdm aanevffeky pdaqlphqiq vrpfnsektq smrslnpedi dqlvtisgmi
      301 irtsnlipem reaffrctac afveaveidr griaepvtcr hcsakysctl ihnrsqfsdk
      361 qmvklqeape dmpagqtpht aliyahndlv davqpgdrit vtgiyrasav rvnprqrtvk
      421 avykthidav hfrkldnkrl yedsedakdc hftperieql krlsrlpdiy erlaralaps
      481 iyenedikkg illqlfggtr kdfadsgrgk frseinillc gdpgtsksql lqyvynlvpr
      541 gqytsgkgss avgltayvtk dpetkqlvlq tgalvlsdng iccidefdkm sdstrsilhe
      601 vmeqqtlsia kagiicqlna rtsilaaanp vesqwntnkt iieniqlpht llsrfdlifl
      661 mldpqdvryd rslarhlvsl yykkpeeaee eqmelglmkd yiayartyvh pqmseeagqa
      721 lieayvdmrr agsgrgqvsa yprqleslir lseahakvrf snvvelvdve eakrlhreal
      781 kqsatdpasg kidisilttg vsassrqrra qmasalrkml etksksqsla ynkvfadfkd
      841 qsdvmitrem feetlkdlqd dgflvlvgrt siklc
//
LOCUS       XP_077503374             875 aa            linear   INV 25-NOV-2025
DEFINITION  disc proliferation abnormal isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503374
VERSION     XP_077503374.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647248.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..875
                     /product="disc proliferation abnormal isoform X1"
                     /calculated_mol_wt=97761
     Region          173..275
                     /region_name="MCM_N"
                     /note="MCM N-terminal domain; pfam14551"
                     /db_xref="CDD:464206"
     Region          278..781
                     /region_name="MCM"
                     /note="minichromosome maintenance proteins; smart00350"
                     /db_xref="CDD:214631"
     Site            order(479,501,523..524,529,532,536,543..545,547..548,
                     550..551,553,557,562,569..571,575,578,586,589,596,
                     599..600,604,608,610..612,614..617,619,629,633..634,650,
                     663,670..671,674,678..679,681..682,713,718,721..722,725,
                     729,741..742,745..746,749)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:350661"
     Site            order(480..482,523..529,603,654,673,677,742..743)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350661"
     Site            order(550,552,557,562,611..612)
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:350661"
     CDS             1..875
                     /gene="dpa"
                     /coded_by="XM_077647248.1:134..2761"
                     /db_xref="GeneID:144113878"
ORIGIN      
        1 msspsrtprr ntrsasrepr tpsrglrstp srkntparst rgtpsrrqrd gtgtdggaag
       61 vsaapsssid gvnevppssp yqrnlpvfte ldlssplhyg tpssatgtpr snargtpirh
      121 rtdirndrrm rqvaigasdg dtgeqtgava tsdsgtgpql viwgtdvvvs hckekfkqfl
      181 rtfaevnlat delmdgvdpv qplylqklee lymleepfln vncqhvanfd adlyrqlkcy
      241 pqeviptfdm aanevffeky pdaqlphqiq vrpfnsektq smrslnpedi dqlvtisgmi
      301 irtsnlipem reaffrctac afveaveidr griaepvtcr hcsakysctl ihnrsqfsdk
      361 qmvklqeape dmpagqtpht aliyahndlv davqpgdrit vtgiyrasav rvnprqrtvk
      421 avykthidav hfrkldnkrl yedsedakdc hftperieql krlsrlpdiy erlaralaps
      481 iyenedikkg illqlfggtr kdfadsgrgk frseinillc gdpgtsksql lqyvynlvpr
      541 gqytsgkgss avgltayvtk dpetkqlvlq tgalvlsdng iccidefdkm sdstrsilhe
      601 vmeqqtlsia kagiicqlna rtsilaaanp vesqwntnkt iieniqlpht llsrfdlifl
      661 mldpqdvryd rslarhlvsl yykkpeeaee eqmelglmkd yiayartyvh pqmseeagqa
      721 lieayvdmrr agsgrgqvsa yprqleslir lseahakvrf snvvelvdve eakrlhreal
      781 kqsatdpasg kidisilttg vsassrqrra qmasalrkml etksksqsla ynkvfadfkd
      841 qsdvmitrem feetlkdlqd dgflvlvgrt siklc
//
LOCUS       XP_077503375             874 aa            linear   INV 25-NOV-2025
DEFINITION  disc proliferation abnormal isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503375
VERSION     XP_077503375.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647249.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..874
                     /product="disc proliferation abnormal isoform X2"
                     /calculated_mol_wt=97690
     Region          172..274
                     /region_name="MCM_N"
                     /note="MCM N-terminal domain; pfam14551"
                     /db_xref="CDD:464206"
     Region          277..780
                     /region_name="MCM"
                     /note="minichromosome maintenance proteins; smart00350"
                     /db_xref="CDD:214631"
     Site            order(478,500,522..523,528,531,535,542..544,546..547,
                     549..550,552,556,561,568..570,574,577,585,588,595,
                     598..599,603,607,609..611,613..616,618,628,632..633,649,
                     662,669..670,673,677..678,680..681,712,717,720..721,724,
                     728,740..741,744..745,748)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:350661"
     Site            order(479..481,522..528,602,653,672,676,741..742)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350661"
     Site            order(549,551,556,561,610..611)
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:350661"
     CDS             1..874
                     /gene="dpa"
                     /coded_by="XM_077647249.1:169..2793"
                     /db_xref="GeneID:144113878"
ORIGIN      
        1 msspsrtprr ntrsasrepr tpsrglrstp srkntprstr gtpsrrqrdg tgtdggaagv
       61 saapsssidg vnevppsspy qrnlpvftel dlssplhygt pssatgtprs nargtpirhr
      121 tdirndrrmr qvaigasdgd tgeqtgavat sdsgtgpqlv iwgtdvvvsh ckekfkqflr
      181 tfaevnlatd elmdgvdpvq plylqkleel ymleepflnv ncqhvanfda dlyrqlkcyp
      241 qeviptfdma anevffekyp daqlphqiqv rpfnsektqs mrslnpedid qlvtisgmii
      301 rtsnlipemr eaffrctaca fveaveidrg riaepvtcrh csakysctli hnrsqfsdkq
      361 mvklqeaped mpagqtphta liyahndlvd avqpgdritv tgiyrasavr vnprqrtvka
      421 vykthidavh frkldnkrly edsedakdch ftperieqlk rlsrlpdiye rlaralapsi
      481 yenedikkgi llqlfggtrk dfadsgrgkf rseinillcg dpgtsksqll qyvynlvprg
      541 qytsgkgssa vgltayvtkd petkqlvlqt galvlsdngi ccidefdkms dstrsilhev
      601 meqqtlsiak agiicqlnar tsilaaanpv esqwntnkti ieniqlphtl lsrfdliflm
      661 ldpqdvrydr slarhlvsly ykkpeeaeee qmelglmkdy iayartyvhp qmseeagqal
      721 ieayvdmrra gsgrgqvsay prqleslirl seahakvrfs nvvelvdvee akrlhrealk
      781 qsatdpasgk idisilttgv sassrqrraq masalrkmle tksksqslay nkvfadfkdq
      841 sdvmitremf eetlkdlqdd gflvlvgrts iklc
//
LOCUS       XP_077503376             874 aa            linear   INV 25-NOV-2025
DEFINITION  disc proliferation abnormal isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503376
VERSION     XP_077503376.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647250.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..874
                     /product="disc proliferation abnormal isoform X2"
                     /calculated_mol_wt=97690
     Region          172..274
                     /region_name="MCM_N"
                     /note="MCM N-terminal domain; pfam14551"
                     /db_xref="CDD:464206"
     Region          277..780
                     /region_name="MCM"
                     /note="minichromosome maintenance proteins; smart00350"
                     /db_xref="CDD:214631"
     Site            order(478,500,522..523,528,531,535,542..544,546..547,
                     549..550,552,556,561,568..570,574,577,585,588,595,
                     598..599,603,607,609..611,613..616,618,628,632..633,649,
                     662,669..670,673,677..678,680..681,712,717,720..721,724,
                     728,740..741,744..745,748)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:350661"
     Site            order(479..481,522..528,602,653,672,676,741..742)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350661"
     Site            order(549,551,556,561,610..611)
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:350661"
     CDS             1..874
                     /gene="dpa"
                     /coded_by="XM_077647250.1:134..2758"
                     /db_xref="GeneID:144113878"
ORIGIN      
        1 msspsrtprr ntrsasrepr tpsrglrstp srkntprstr gtpsrrqrdg tgtdggaagv
       61 saapsssidg vnevppsspy qrnlpvftel dlssplhygt pssatgtprs nargtpirhr
      121 tdirndrrmr qvaigasdgd tgeqtgavat sdsgtgpqlv iwgtdvvvsh ckekfkqflr
      181 tfaevnlatd elmdgvdpvq plylqkleel ymleepflnv ncqhvanfda dlyrqlkcyp
      241 qeviptfdma anevffekyp daqlphqiqv rpfnsektqs mrslnpedid qlvtisgmii
      301 rtsnlipemr eaffrctaca fveaveidrg riaepvtcrh csakysctli hnrsqfsdkq
      361 mvklqeaped mpagqtphta liyahndlvd avqpgdritv tgiyrasavr vnprqrtvka
      421 vykthidavh frkldnkrly edsedakdch ftperieqlk rlsrlpdiye rlaralapsi
      481 yenedikkgi llqlfggtrk dfadsgrgkf rseinillcg dpgtsksqll qyvynlvprg
      541 qytsgkgssa vgltayvtkd petkqlvlqt galvlsdngi ccidefdkms dstrsilhev
      601 meqqtlsiak agiicqlnar tsilaaanpv esqwntnkti ieniqlphtl lsrfdliflm
      661 ldpqdvrydr slarhlvsly ykkpeeaeee qmelglmkdy iayartyvhp qmseeagqal
      721 ieayvdmrra gsgrgqvsay prqleslirl seahakvrfs nvvelvdvee akrlhrealk
      781 qsatdpasgk idisilttgv sassrqrraq masalrkmle tksksqslay nkvfadfkdq
      841 sdvmitremf eetlkdlqdd gflvlvgrts iklc
//
LOCUS       XP_077503377            3000 aa            linear   INV 25-NOV-2025
DEFINITION  neural cadherin isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503377
VERSION     XP_077503377.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647251.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..3000
                     /product="neural cadherin isoform X1"
                     /calculated_mol_wt=335777
     Region          127..226
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(134..135,192,194,222,224..225)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          234..318
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          450..527
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          557..653
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(563..564,614,616,649,651..652)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          666..753
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(669..670,717,719,750,752..753)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          762..864
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(769..770,823,860,862..863)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          873..974
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(880..881,935,937,970,972..973)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          982..1075
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          1091..1194
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1095..1096,1158,1160,1191,1193..1194)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1206..1310
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1209..1210,1264,1266,1306,1308..1309)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1319..1410
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1325..1326,1376,1378,1406,1408..1409)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1418..1525
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1425,1428,1485,1487,1521,1523..1524)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1533..1635
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1540..1541,1601,1603,1632,1634..1635)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1656..1755
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1659..1660,1711,1713,1751,1753..1754)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1764..1860
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1770..1771,1823,1825,1856,1858..1859)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1868..1977
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1874..1875,1940,1942,1973,1975..1976)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2241..2278
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          2281..2462
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          2498..2523
                     /region_name="EGF"
                     /note="Epidermal growth factor-like domain; smart00181"
                     /db_xref="CDD:214544"
     Region          2531..2691
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          2759..2796
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(2759,2762,2776)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          2934..2993
                     /region_name="CADH_Y-type_LIR"
                     /note="Cadherin, Y-type LIR-motif; pfam01049"
                     /db_xref="CDD:460041"
     CDS             1..3000
                     /gene="CadN"
                     /coded_by="XM_077647251.1:249..9251"
                     /db_xref="GeneID:144113880"
ORIGIN      
        1 malpprlica aavlvvlwvp ghsktssdav lvlpddvypg lsvkqlpsvg qsfaltgepr
       61 vtrcfsllsd gllmvasnvs hlvgkpgklv ltetnfpswp lepqdrvqel lvhvipknkl
      121 lkfpetefrg tvlenecagt tvleldgatk dasrsvkyel lpdedgghee ftvsrlydns
      181 trlavrtrrp ldrehrrrye lklrasdgvd saltrllvnv ldvndndpva drdsyvfrva
      241 rdaepysivg nvsasdadgd svsfrlaqrh pfftvvpktg elmvtrglep ksylmavrvg
      301 drgkpqrfgr avavtvevse pepvapeprc leepetrvrr stvtirptkt yrfeetkgrd
      361 vnktvftldk kavdetfele kpdrwvyvsp ngdvrvkerw dyeqldkeks idfwvlarsp
      421 graekerqri iiriadvnde ppyfinrpip mqavvqlnap agtpvfklqa rdpdtdhnir
      481 ysivrdrsgn rfdvdahsge vvtlgsapfm ldkeyvlyvk aedlngqvgd rqyqstreer
      541 lsvvggkrqp qfyvpfyeav ipenqkkdsd iievkaksfa dreirytlra qgkgagtfni
      601 gptsgvvkla keldyedlrq pksyslivta tedsggfsts veltikvtdv ndnaprfelp
      661 dyqahnvded iavgtsilqv satdmdtgrn aeltysldke dftidsrgvv ysnrrldadv
      721 nntyvltvra tdrgeppltg tatiriyten kndeapkfsq dvytpnvden agpstlvttv
      781 vasdkdgdni lfgfvnsgtt sgmfqieert gvirlingpi hldkdkyeln vtarddgacc
      841 rngaltphts talvvvfitd vndnkpvfee cqtytpkvee gaqsgtsvik vkardldkgh
      901 ngqvrysivq qpnqkgtkfs vdeltgeirt nkvfdregdd grfvsvtvka tdrgsppleg
      961 vcsfkveitd indnpplfdr qeyrenvkqd tqvgihilrv sasdedadnn gaivynltap
     1021 ydpehlayfs inpdsgwisl qkaldrdqyq lraialdkgv pqhqatvevi idvvdrannp
     1081 piwdqpvygp ifikenlevg trviaikars gipqnpdvfy tlmkgsteqt nkkdtfylnq
     1141 klenrqtvae lvvnypldye riqqynltvr venngiqqla seatvyivle dvndeiplfi
     1201 ereqetvleg lppgtkvtqv qavdkdgtyp nnkvyyaies rdhgdkyfsi dretgdiytk
     1261 vefdreekma yailvraedg apsarphmtd nrpnsvtkyi rigigdkndn ppyfgqalye
     1321 aevnededvq htvitvtakd kdessrirye itqgniggaf avknetgaiy vagpldyetr
     1381 kefrlrlvas dnlnenhttv likvkdvndn apifdrptye aqiteendrn lpqkilqvta
     1441 tdgdkdresl ivyfltgqgv dedpgnskfa intttgeiyv lkpldrdlph grsqwrftvf
     1501 aedeggnglv gfadvlvnlk dvndnapffp saiytgnvte ngtagmtvmt mtatdyddpn
     1561 egnnarlays ieqnqvneng eliftideet gvistavccl dretnpeyti kvvavdgggl
     1621 kgtgtatiki kdindmppef tkkdwfvdve eteadnipen pilvvsvndg dlletnrfsy
     1681 kvidntfgsd kftmvtnsdg tgslkiakpl dyedlqqrfg fnitiqvsdn ggestdpyha
     1741 dyakvkvrvk dindnkpefe rpnievsvpe nstvgsslat fkatdadqgg ksrvsymidr
     1801 ssdkkrqfki npngvveiqr tldrediprh qvkilaiddg vpsrtatatl tvvvsdindn
     1861 pprfqydyrp vipehtppqk vqeilatddd drsknngppf tfrmdpnape liqqffrveh
     1921 dptgangdgm avvrsretfd retqkeylvp ilikdngnps ltgtstltvi igdvndnrmh
     1981 pgsksifvyn fkgespptki grihvedldd wdlpdktffw ennnahpnfd mdghtgmitm
     2041 lnstgagtyh lrflvydrmh tmdahanvtv tvkeipeeai ynsgsirvhg itdedfirvw
     2101 dwkkqrqvks kyeifrdlia eriktkpynv difsvilkqs rppitdirfs ahgspyykas
     2161 llngvvavnr reieekvgin itmvgidecl lenhncegsc tnvlmidrnp vmvnanrtsf
     2221 vgvntwvkak ctcgardfse iescrkrppp cynngrcvdt ytgiscrcqs gfhgpqcqqt
     2281 trhfngkgwa wfpplqqced shlslefmte rptglllyng pisrpdpgev viqdfislel
     2341 nagrprllld fgsgtaelfv dveealnngn whrldvfwdr qsvrlmvdnc msarthegnq
     2401 tidrsrcens tqvpefnefl nvntplqlgg thvggkdlga yawrhrhtte gfqgciknvv
     2461 hnsemydlgs pgssynsvtg cppaesscse aallhqscgh ghclgtykga ecvcdpgwhg
     2521 pncdretqsk mfqqssyiky alpfdphpyk tdiqlqfrtr qkhgelfrit skhgreycil
     2581 eirdkklrfr fnldrsrase erelwlpwlw vndgqwhtvr alrygasasl aldggsgrrf
     2641 nelldwhsph qhmwvekqnv iaggdvqyvg pgvtvvdndy qegcmndirl dqhylpmesg
     2701 seyaavvewr nlidgcpsnn pchgiscprp fvcvdlwmlh ecrcvegftp tedrldcedk
     2761 decldepcgy aghctnlphg qghtclches lechncscfd vgagarmtnt slalgleall
     2821 iilaclvvyl llvlvivayt rsrrsdqkfg hggvdddvre niisyddegg geddmnaydi
     2881 tplripvdas gmqigakpgp ekapplqkev rvrepptgah pdvgdfirdh ldkadqdpga
     2941 ppvddvrnya yegggstags lsslasetdd heqrfdylng wgprfqklad mygqgeseed
//
LOCUS       XP_077503378            3000 aa            linear   INV 25-NOV-2025
DEFINITION  neural cadherin isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503378
VERSION     XP_077503378.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647252.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..3000
                     /product="neural cadherin isoform X2"
                     /calculated_mol_wt=335484
     Region          127..226
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(134..135,192,194,222,224..225)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          234..318
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          450..527
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          557..653
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(563..564,614,616,649,651..652)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          666..753
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(669..670,717,719,750,752..753)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          762..864
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(769..770,823,860,862..863)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          873..974
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(880..881,935,937,970,972..973)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          982..1075
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          1091..1194
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1095..1096,1158,1160,1191,1193..1194)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1206..1310
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1209..1210,1264,1266,1306,1308..1309)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1319..1409
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1325..1326,1376,1378,1406,1408..1409)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1418..1525
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1425..1426,1485,1487,1521,1523..1524)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1533..1635
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1540..1541,1601,1603,1632,1634..1635)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1656..1755
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1659..1660,1711,1713,1751,1753..1754)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1764..1860
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1770..1771,1823,1825,1856,1858..1859)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1868..1977
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1874..1875,1940,1942,1973,1975..1976)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2241..2278
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          2281..2462
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          2498..2523
                     /region_name="EGF"
                     /note="Epidermal growth factor-like domain; smart00181"
                     /db_xref="CDD:214544"
     Region          2531..2691
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          2759..2796
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(2759,2762,2776)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          2934..2993
                     /region_name="CADH_Y-type_LIR"
                     /note="Cadherin, Y-type LIR-motif; pfam01049"
                     /db_xref="CDD:460041"
     CDS             1..3000
                     /gene="CadN"
                     /coded_by="XM_077647252.1:249..9251"
                     /db_xref="GeneID:144113880"
ORIGIN      
        1 malpprlica aavlvvlwvp ghsktssdav lvlpddvypg lsvkqlpsvg qsfaltgepr
       61 vtrcfsllsd gllmvasnvs hlvgkpgklv ltetnfpswp lepqdrvqel lvhvipknkl
      121 lkfpetefrg tvlenecagt tvleldgatk dasrsvkyel lpdedgghee ftvsrlydns
      181 trlavrtrrp ldrehrrrye lklrasdgvd saltrllvnv ldvndndpva drdsyvfrva
      241 rdaepysivg nvsasdadgd svsfrlaqrh pfftvvpktg elmvtrglep ksylmavrvg
      301 drgkpqrfgr avavtvevse pepvapeprc leepetrvrr stvtirptkt yrfeetkgrd
      361 vnktvftldk kavdetfele kpdrwvyvsp ngdvrvkerw dyeqldkeks idfwvlarsp
      421 graekerqri iiriadvnde ppyfinrpip mqavvqlnap agtpvfklqa rdpdtdhnir
      481 ysivrdrsgn rfdvdahsge vvtlgsapfm ldkeyvlyvk aedlngqvgd rqyqstreer
      541 lsvvggkrqp qfyvpfyeav ipenqkkdsd iievkaksfa dreirytlra qgkgagtfni
      601 gptsgvvkla keldyedlrq pksyslivta tedsggfsts veltikvtdv ndnaprfelp
      661 dyqahnvded iavgtsilqv satdmdtgrn aeltysldke dftidsrgvv ysnrrldadv
      721 nntyvltvra tdrgeppltg tatiriyten kndeapkfsq dvytpnvden agpstlvttv
      781 vasdkdgdni lfgfvnsgtt sgmfqieert gvirlingpi hldkdkyeln vtarddgacc
      841 rngaltphts talvvvfitd vndnkpvfee cqtytpkvee gaqsgtsvik vkardldkgh
      901 ngqvrysivq qpnqkgtkfs vdeltgeirt nkvfdregdd grfvsvtvka tdrgsppleg
      961 vcsfkveitd indnpplfdr qeyrenvkqd tqvgihilrv sasdedadnn gaivynltap
     1021 ydpehlayfs inpdsgwisl qkaldrdqyq lraialdkgv pqhqatvevi idvvdrannp
     1081 piwdqpvygp ifikenlevg trviaikars gipqnpdvfy tlmkgsteqt nkkdtfylnq
     1141 klenrqtvae lvvnypldye riqqynltvr venngiqqla seatvyivle dvndeiplfi
     1201 ereqetvleg lppgtkvtqv qavdkdgtyp nnkvyyaies rdhgdkyfsi dretgdiytk
     1261 vefdreekma yailvraedg apsarphmtd nrpnsvtkyi rigigdkndn ppyfgqalye
     1321 aevnededvq htvitvtakd kdessrirye itqgniggaf avknetgaiy vagpldyetr
     1381 kefnltlvas dgfhegnttv lihvkdvndl ppvfgqpsym ttieeevshn lpmkimtvta
     1441 tdgdkdresl ivyfltgqgv dedpgnskfa intttgeiyv lkpldrdlph grsqwrftvf
     1501 aedeggnglv gfadvlvnlk dvndnapffp saiytgnvte ngtagmtvmt mtatdyddpn
     1561 egnnarlays ieqnqvneng eliftideet gvistavccl dretnpeyti kvvavdgggl
     1621 kgtgtatiki kdindmppef tkkdwfvdve eteadnipen pilvvsvndg dlletnrfsy
     1681 kvidntfgsd kftmvtnsdg tgslkiakpl dyedlqqrfg fnitiqvsdn ggestdpyha
     1741 dyakvkvrvk dindnkpefe rpnievsvpe nstvgsslat fkatdadqgg ksrvsymidr
     1801 ssdkkrqfki npngvveiqr tldrediprh qvkilaiddg vpsrtatatl tvvvsdindn
     1861 pprfqydyrp vipehtppqk vqeilatddd drsknngppf tfrmdpnape liqqffrveh
     1921 dptgangdgm avvrsretfd retqkeylvp ilikdngnps ltgtstltvi igdvndnrmh
     1981 pgsksifvyn fkgespptki grihvedldd wdlpdktffw ennnahpnfd mdghtgmitm
     2041 lnstgagtyh lrflvydrmh tmdahanvtv tvkeipeeai ynsgsirvhg itdedfirvw
     2101 dwkkqrqvks kyeifrdlia eriktkpynv difsvilkqs rppitdirfs ahgspyykas
     2161 llngvvavnr reieekvgin itmvgidecl lenhncegsc tnvlmidrnp vmvnanrtsf
     2221 vgvntwvkak ctcgardfse iescrkrppp cynngrcvdt ytgiscrcqs gfhgpqcqqt
     2281 trhfngkgwa wfpplqqced shlslefmte rptglllyng pisrpdpgev viqdfislel
     2341 nagrprllld fgsgtaelfv dveealnngn whrldvfwdr qsvrlmvdnc msarthegnq
     2401 tidrsrcens tqvpefnefl nvntplqlgg thvggkdlga yawrhrhtte gfqgciknvv
     2461 hnsemydlgs pgssynsvtg cppaesscse aallhqscgh ghclgtykga ecvcdpgwhg
     2521 pncdretqsk mfqqssyiky alpfdphpyk tdiqlqfrtr qkhgelfrit skhgreycil
     2581 eirdkklrfr fnldrsrase erelwlpwlw vndgqwhtvr alrygasasl aldggsgrrf
     2641 nelldwhsph qhmwvekqnv iaggdvqyvg pgvtvvdndy qegcmndirl dqhylpmesg
     2701 seyaavvewr nlidgcpsnn pchgiscprp fvcvdlwmlh ecrcvegftp tedrldcedk
     2761 decldepcgy aghctnlphg qghtclches lechncscfd vgagarmtnt slalgleall
     2821 iilaclvvyl llvlvivayt rsrrsdqkfg hggvdddvre niisyddegg geddmnaydi
     2881 tplripvdas gmqigakpgp ekapplqkev rvrepptgah pdvgdfirdh ldkadqdpga
     2941 ppvddvrnya yegggstags lsslasetdd heqrfdylng wgprfqklad mygqgeseed
//
LOCUS       XP_077503379            2993 aa            linear   INV 25-NOV-2025
DEFINITION  neural cadherin isoform X3 [Amblyomma americanum].
ACCESSION   XP_077503379
VERSION     XP_077503379.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647253.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2993
                     /product="neural cadherin isoform X3"
                     /calculated_mol_wt=334845
     Region          127..226
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(134..135,192,194,222,224..225)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          234..318
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          450..527
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          557..653
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(563..564,614,616,649,651..652)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          666..753
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(669..670,717,719,750,752..753)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          762..864
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(769..770,823,860,862..863)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          873..974
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(880..881,935,937,970,972..973)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          982..1075
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          1091..1194
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1095..1096,1158,1160,1191,1193..1194)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1206..1310
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1209..1210,1264,1266,1306,1308..1309)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1319..1410
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1325..1326,1376,1378,1406,1408..1409)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1418..1525
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1425,1428,1485,1487,1521,1523..1524)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1533..1635
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1540..1541,1601,1603,1632,1634..1635)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1656..1755
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1659..1660,1711,1713,1751,1753..1754)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1764..1860
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1770..1771,1823,1825,1856,1858..1859)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1868..1977
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1874..1875,1940,1942,1973,1975..1976)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2241..2278
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          2281..2462
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          2498..2523
                     /region_name="EGF"
                     /note="Epidermal growth factor-like domain; smart00181"
                     /db_xref="CDD:214544"
     Region          2531..2691
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          2713..2758
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain; smart00179"
                     /db_xref="CDD:214542"
     Region          2759..2796
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(2759,2762,2776)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          2927..2986
                     /region_name="CADH_Y-type_LIR"
                     /note="Cadherin, Y-type LIR-motif; pfam01049"
                     /db_xref="CDD:460041"
     CDS             1..2993
                     /gene="CadN"
                     /coded_by="XM_077647253.1:249..9230"
                     /db_xref="GeneID:144113880"
ORIGIN      
        1 malpprlica aavlvvlwvp ghsktssdav lvlpddvypg lsvkqlpsvg qsfaltgepr
       61 vtrcfsllsd gllmvasnvs hlvgkpgklv ltetnfpswp lepqdrvqel lvhvipknkl
      121 lkfpetefrg tvlenecagt tvleldgatk dasrsvkyel lpdedgghee ftvsrlydns
      181 trlavrtrrp ldrehrrrye lklrasdgvd saltrllvnv ldvndndpva drdsyvfrva
      241 rdaepysivg nvsasdadgd svsfrlaqrh pfftvvpktg elmvtrglep ksylmavrvg
      301 drgkpqrfgr avavtvevse pepvapeprc leepetrvrr stvtirptkt yrfeetkgrd
      361 vnktvftldk kavdetfele kpdrwvyvsp ngdvrvkerw dyeqldkeks idfwvlarsp
      421 graekerqri iiriadvnde ppyfinrpip mqavvqlnap agtpvfklqa rdpdtdhnir
      481 ysivrdrsgn rfdvdahsge vvtlgsapfm ldkeyvlyvk aedlngqvgd rqyqstreer
      541 lsvvggkrqp qfyvpfyeav ipenqkkdsd iievkaksfa dreirytlra qgkgagtfni
      601 gptsgvvkla keldyedlrq pksyslivta tedsggfsts veltikvtdv ndnaprfelp
      661 dyqahnvded iavgtsilqv satdmdtgrn aeltysldke dftidsrgvv ysnrrldadv
      721 nntyvltvra tdrgeppltg tatiriyten kndeapkfsq dvytpnvden agpstlvttv
      781 vasdkdgdni lfgfvnsgtt sgmfqieert gvirlingpi hldkdkyeln vtarddgacc
      841 rngaltphts talvvvfitd vndnkpvfee cqtytpkvee gaqsgtsvik vkardldkgh
      901 ngqvrysivq qpnqkgtkfs vdeltgeirt nkvfdregdd grfvsvtvka tdrgsppleg
      961 vcsfkveitd indnpplfdr qeyrenvkqd tqvgihilrv sasdedadnn gaivynltap
     1021 ydpehlayfs inpdsgwisl qkaldrdqyq lraialdkgv pqhqatvevi idvvdrannp
     1081 piwdqpvygp ifikenlevg trviaikars gipqnpdvfy tlmkgsteqt nkkdtfylnq
     1141 klenrqtvae lvvnypldye riqqynltvr venngiqqla seatvyivle dvndeiplfi
     1201 ereqetvleg lppgtkvtqv qavdkdgtyp nnkvyyaies rdhgdkyfsi dretgdiytk
     1261 vefdreekma yailvraedg apsarphmtd nrpnsvtkyi rigigdkndn ppyfgqalye
     1321 aevnededvq htvitvtakd kdessrirye itqgniggaf avknetgaiy vagpldyetr
     1381 kefrlrlvas dnlnenhttv likvkdvndn apifdrptye aqiteendrn lpqkilqvta
     1441 tdgdkdresl ivyfltgqgv dedpgnskfa intttgeiyv lkpldrdlph grsqwrftvf
     1501 aedeggnglv gfadvlvnlk dvndnapffp saiytgnvte ngtagmtvmt mtatdyddpn
     1561 egnnarlays ieqnqvneng eliftideet gvistavccl dretnpeyti kvvavdgggl
     1621 kgtgtatiki kdindmppef tkkdwfvdve eteadnipen pilvvsvndg dlletnrfsy
     1681 kvidntfgsd kftmvtnsdg tgslkiakpl dyedlqqrfg fnitiqvsdn ggestdpyha
     1741 dyakvkvrvk dindnkpefe rpnievsvpe nstvgsslat fkatdadqgg ksrvsymidr
     1801 ssdkkrqfki npngvveiqr tldrediprh qvkilaiddg vpsrtatatl tvvvsdindn
     1861 pprfqydyrp vipehtppqk vqeilatddd drsknngppf tfrmdpnape liqqffrveh
     1921 dptgangdgm avvrsretfd retqkeylvp ilikdngnps ltgtstltvi igdvndnrmh
     1981 pgsksifvyn fkgespptki grihvedldd wdlpdktffw ennnahpnfd mdghtgmitm
     2041 lnstgagtyh lrflvydrmh tmdahanvtv tvkeipeeai ynsgsirvhg itdedfirvw
     2101 dwkkqrqvks kyeifrdlia eriktkpynv difsvilkqs rppitdirfs ahgspyykas
     2161 llngvvavnr reieekvgin itmvgidecl lenhncegsc tnvlmidrnp vmvnanrtsf
     2221 vgvntwvkak ctcgardfse iescrkrppp cynngrcvdt ytgiscrcqs gfhgpqcqqt
     2281 trhfngkgwa wfpplqqced shlslefmte rptglllyng pisrpdpgev viqdfislel
     2341 nagrprllld fgsgtaelfv dveealnngn whrldvfwdr qsvrlmvdnc msarthegnq
     2401 tidrsrcens tqvpefnefl nvntplqlgg thvggkdlga yawrhrhtte gfqgciknvv
     2461 hnsemydlgs pgssynsvtg cppaesscse aallhqscgh ghclgtykga ecvcdpgwhg
     2521 pncdretqsk mfqqssyiky alpfdphpyk tdiqlqfrtr qkhgelfrit skhgreycil
     2581 eirdkklrfr fnldrsrase erelwlpwlw vndgqwhtvr alrygasasl aldggsgrrf
     2641 nelldwhsph qhmwvekqnv iaggdvqyvg pgvtvvdndy qegcmndirl dqhylpmesg
     2701 seyaavvewr nlidgcpsnn pchgiscprp fvcvdlwmlh ecrcspgfav tengknctda
     2761 decltepcln ggtcmnrphg egfyclcpdg fggdlcgalr qekimrlsma alaailvcll
     2821 nililvlviv aytrsrrsdq kfghggvddd vreniisydd egggeddmna yditplripv
     2881 dasgmqigak pgpekapplq kevrvreppt gahpdvgdfi rdhldkadqd pgappvddvr
     2941 nyayegggst agslsslase tddheqrfdy lngwgprfqk ladmygqges eed
//
LOCUS       XP_077503380            2993 aa            linear   INV 25-NOV-2025
DEFINITION  neural cadherin isoform X4 [Amblyomma americanum].
ACCESSION   XP_077503380
VERSION     XP_077503380.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647254.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2993
                     /product="neural cadherin isoform X4"
                     /calculated_mol_wt=334553
     Region          127..226
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(134..135,192,194,222,224..225)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          234..318
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          450..527
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          557..653
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(563..564,614,616,649,651..652)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          666..753
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(669..670,717,719,750,752..753)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          762..864
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(769..770,823,860,862..863)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          873..974
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(880..881,935,937,970,972..973)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          982..1075
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          1091..1194
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1095..1096,1158,1160,1191,1193..1194)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1206..1310
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1209..1210,1264,1266,1306,1308..1309)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1319..1409
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1325..1326,1376,1378,1406,1408..1409)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1418..1525
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1425..1426,1485,1487,1521,1523..1524)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1533..1635
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1540..1541,1601,1603,1632,1634..1635)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1656..1755
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1659..1660,1711,1713,1751,1753..1754)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1764..1860
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1770..1771,1823,1825,1856,1858..1859)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1868..1977
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1874..1875,1940,1942,1973,1975..1976)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2241..2278
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          2281..2462
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          2498..2523
                     /region_name="EGF"
                     /note="Epidermal growth factor-like domain; smart00181"
                     /db_xref="CDD:214544"
     Region          2531..2691
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          2713..2758
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain; smart00179"
                     /db_xref="CDD:214542"
     Region          2759..2796
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(2759,2762,2776)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          2927..2986
                     /region_name="CADH_Y-type_LIR"
                     /note="Cadherin, Y-type LIR-motif; pfam01049"
                     /db_xref="CDD:460041"
     CDS             1..2993
                     /gene="CadN"
                     /coded_by="XM_077647254.1:249..9230"
                     /db_xref="GeneID:144113880"
ORIGIN      
        1 malpprlica aavlvvlwvp ghsktssdav lvlpddvypg lsvkqlpsvg qsfaltgepr
       61 vtrcfsllsd gllmvasnvs hlvgkpgklv ltetnfpswp lepqdrvqel lvhvipknkl
      121 lkfpetefrg tvlenecagt tvleldgatk dasrsvkyel lpdedgghee ftvsrlydns
      181 trlavrtrrp ldrehrrrye lklrasdgvd saltrllvnv ldvndndpva drdsyvfrva
      241 rdaepysivg nvsasdadgd svsfrlaqrh pfftvvpktg elmvtrglep ksylmavrvg
      301 drgkpqrfgr avavtvevse pepvapeprc leepetrvrr stvtirptkt yrfeetkgrd
      361 vnktvftldk kavdetfele kpdrwvyvsp ngdvrvkerw dyeqldkeks idfwvlarsp
      421 graekerqri iiriadvnde ppyfinrpip mqavvqlnap agtpvfklqa rdpdtdhnir
      481 ysivrdrsgn rfdvdahsge vvtlgsapfm ldkeyvlyvk aedlngqvgd rqyqstreer
      541 lsvvggkrqp qfyvpfyeav ipenqkkdsd iievkaksfa dreirytlra qgkgagtfni
      601 gptsgvvkla keldyedlrq pksyslivta tedsggfsts veltikvtdv ndnaprfelp
      661 dyqahnvded iavgtsilqv satdmdtgrn aeltysldke dftidsrgvv ysnrrldadv
      721 nntyvltvra tdrgeppltg tatiriyten kndeapkfsq dvytpnvden agpstlvttv
      781 vasdkdgdni lfgfvnsgtt sgmfqieert gvirlingpi hldkdkyeln vtarddgacc
      841 rngaltphts talvvvfitd vndnkpvfee cqtytpkvee gaqsgtsvik vkardldkgh
      901 ngqvrysivq qpnqkgtkfs vdeltgeirt nkvfdregdd grfvsvtvka tdrgsppleg
      961 vcsfkveitd indnpplfdr qeyrenvkqd tqvgihilrv sasdedadnn gaivynltap
     1021 ydpehlayfs inpdsgwisl qkaldrdqyq lraialdkgv pqhqatvevi idvvdrannp
     1081 piwdqpvygp ifikenlevg trviaikars gipqnpdvfy tlmkgsteqt nkkdtfylnq
     1141 klenrqtvae lvvnypldye riqqynltvr venngiqqla seatvyivle dvndeiplfi
     1201 ereqetvleg lppgtkvtqv qavdkdgtyp nnkvyyaies rdhgdkyfsi dretgdiytk
     1261 vefdreekma yailvraedg apsarphmtd nrpnsvtkyi rigigdkndn ppyfgqalye
     1321 aevnededvq htvitvtakd kdessrirye itqgniggaf avknetgaiy vagpldyetr
     1381 kefnltlvas dgfhegnttv lihvkdvndl ppvfgqpsym ttieeevshn lpmkimtvta
     1441 tdgdkdresl ivyfltgqgv dedpgnskfa intttgeiyv lkpldrdlph grsqwrftvf
     1501 aedeggnglv gfadvlvnlk dvndnapffp saiytgnvte ngtagmtvmt mtatdyddpn
     1561 egnnarlays ieqnqvneng eliftideet gvistavccl dretnpeyti kvvavdgggl
     1621 kgtgtatiki kdindmppef tkkdwfvdve eteadnipen pilvvsvndg dlletnrfsy
     1681 kvidntfgsd kftmvtnsdg tgslkiakpl dyedlqqrfg fnitiqvsdn ggestdpyha
     1741 dyakvkvrvk dindnkpefe rpnievsvpe nstvgsslat fkatdadqgg ksrvsymidr
     1801 ssdkkrqfki npngvveiqr tldrediprh qvkilaiddg vpsrtatatl tvvvsdindn
     1861 pprfqydyrp vipehtppqk vqeilatddd drsknngppf tfrmdpnape liqqffrveh
     1921 dptgangdgm avvrsretfd retqkeylvp ilikdngnps ltgtstltvi igdvndnrmh
     1981 pgsksifvyn fkgespptki grihvedldd wdlpdktffw ennnahpnfd mdghtgmitm
     2041 lnstgagtyh lrflvydrmh tmdahanvtv tvkeipeeai ynsgsirvhg itdedfirvw
     2101 dwkkqrqvks kyeifrdlia eriktkpynv difsvilkqs rppitdirfs ahgspyykas
     2161 llngvvavnr reieekvgin itmvgidecl lenhncegsc tnvlmidrnp vmvnanrtsf
     2221 vgvntwvkak ctcgardfse iescrkrppp cynngrcvdt ytgiscrcqs gfhgpqcqqt
     2281 trhfngkgwa wfpplqqced shlslefmte rptglllyng pisrpdpgev viqdfislel
     2341 nagrprllld fgsgtaelfv dveealnngn whrldvfwdr qsvrlmvdnc msarthegnq
     2401 tidrsrcens tqvpefnefl nvntplqlgg thvggkdlga yawrhrhtte gfqgciknvv
     2461 hnsemydlgs pgssynsvtg cppaesscse aallhqscgh ghclgtykga ecvcdpgwhg
     2521 pncdretqsk mfqqssyiky alpfdphpyk tdiqlqfrtr qkhgelfrit skhgreycil
     2581 eirdkklrfr fnldrsrase erelwlpwlw vndgqwhtvr alrygasasl aldggsgrrf
     2641 nelldwhsph qhmwvekqnv iaggdvqyvg pgvtvvdndy qegcmndirl dqhylpmesg
     2701 seyaavvewr nlidgcpsnn pchgiscprp fvcvdlwmlh ecrcspgfav tengknctda
     2761 decltepcln ggtcmnrphg egfyclcpdg fggdlcgalr qekimrlsma alaailvcll
     2821 nililvlviv aytrsrrsdq kfghggvddd vreniisydd egggeddmna yditplripv
     2881 dasgmqigak pgpekapplq kevrvreppt gahpdvgdfi rdhldkadqd pgappvddvr
     2941 nyayegggst agslsslase tddheqrfdy lngwgprfqk ladmygqges eed
//
LOCUS       XP_077503382             237 aa            linear   INV 25-NOV-2025
DEFINITION  small nuclear ribonucleoprotein-associated protein B-like
            [Amblyomma americanum].
ACCESSION   XP_077503382
VERSION     XP_077503382.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647256.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..237
                     /product="small nuclear ribonucleoprotein-associated
                     protein B-like"
                     /calculated_mol_wt=24282
     Region          5..83
                     /region_name="Sm_B"
                     /note="Sm protein B; cd01717"
                     /db_xref="CDD:212464"
     Site            order(8,14,22..23,32,36,43..44,70..83)
                     /site_type="other"
                     /note="heptamer interface [polypeptide binding]"
                     /db_xref="CDD:212464"
     Site            order(17..23,25..37,39..43)
                     /site_type="other"
                     /note="Sm1 motif"
                     /db_xref="CDD:212464"
     Site            order(20..25,27,33..34,37,39,41,61,66,68,70..80)
                     /site_type="other"
                     /note="putative hexamer interface [polypeptide binding]"
                     /db_xref="CDD:212464"
     Site            order(36..39,49,60..62,74,76)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:212464"
     Site            70..81
                     /site_type="other"
                     /note="Sm2 motif"
                     /db_xref="CDD:212464"
     CDS             1..237
                     /gene="LOC144113881"
                     /coded_by="XM_077647256.1:132..845"
                     /db_xref="GeneID:144113881"
ORIGIN      
        1 mtisknnkml qhinyrmrvi lqdsrifigt fkafdkhmnl ilgdceefrk vkgknqakhg
       61 dreekralgl vllrgenivs ltvegppppe eglprvpipg aapgpgigra agrgipaapv
      121 gaapaglqgp vrgvggpsaq vmapqagrsa plppgavgvs appqvrppaa mgmrgppamm
      181 ggppammgrg mpppppggmp mggrppmgpp ppgmrgpppp gmmrpgpppg mrpptrq
//
LOCUS       XP_077503383             130 aa            linear   INV 25-NOV-2025
DEFINITION  peptide methionine sulfoxide reductase-like [Amblyomma americanum].
ACCESSION   XP_077503383
VERSION     XP_077503383.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647257.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..130
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..130
                     /product="peptide methionine sulfoxide reductase-like"
                     /calculated_mol_wt=14788
     Region          <1..75
                     /region_name="PMSR"
                     /note="Peptide methionine sulfoxide reductase; pfam01625"
                     /db_xref="CDD:460270"
     CDS             1..130
                     /gene="LOC144113882"
                     /coded_by="XM_077647257.1:369..761"
                     /db_xref="GeneID:144113882"
ORIGIN      
        1 mfwdfhdpta chkrqymsai fyhdkeqkai aeeslkrqqs kikkpiatki mpagtfydae
       61 dyhqkyllrr htalceslkk aglknfkesh vaarlngycs gsgtlaafea ereklglsdt
      121 qaalvrqcfk
//
LOCUS       XP_077503384             130 aa            linear   INV 25-NOV-2025
DEFINITION  peptide methionine sulfoxide reductase-like [Amblyomma americanum].
ACCESSION   XP_077503384
VERSION     XP_077503384.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647258.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..130
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..130
                     /product="peptide methionine sulfoxide reductase-like"
                     /calculated_mol_wt=14788
     Region          <1..75
                     /region_name="PMSR"
                     /note="Peptide methionine sulfoxide reductase; pfam01625"
                     /db_xref="CDD:460270"
     CDS             1..130
                     /gene="LOC144113882"
                     /coded_by="XM_077647258.1:475..867"
                     /db_xref="GeneID:144113882"
ORIGIN      
        1 mfwdfhdpta chkrqymsai fyhdkeqkai aeeslkrqqs kikkpiatki mpagtfydae
       61 dyhqkyllrr htalceslkk aglknfkesh vaarlngycs gsgtlaafea ereklglsdt
      121 qaalvrqcfk
//
LOCUS       XP_077503385             130 aa            linear   INV 25-NOV-2025
DEFINITION  peptide methionine sulfoxide reductase-like [Amblyomma americanum].
ACCESSION   XP_077503385
VERSION     XP_077503385.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647259.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..130
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..130
                     /product="peptide methionine sulfoxide reductase-like"
                     /calculated_mol_wt=14788
     Region          <1..75
                     /region_name="PMSR"
                     /note="Peptide methionine sulfoxide reductase; pfam01625"
                     /db_xref="CDD:460270"
     CDS             1..130
                     /gene="LOC144113882"
                     /coded_by="XM_077647259.1:369..761"
                     /db_xref="GeneID:144113882"
ORIGIN      
        1 mfwdfhdpta chkrqymsai fyhdkeqkai aeeslkrqqs kikkpiatki mpagtfydae
       61 dyhqkyllrr htalceslkk aglknfkesh vaarlngycs gsgtlaafea ereklglsdt
      121 qaalvrqcfk
//
LOCUS       XP_077503386             130 aa            linear   INV 25-NOV-2025
DEFINITION  peptide methionine sulfoxide reductase-like [Amblyomma americanum].
ACCESSION   XP_077503386
VERSION     XP_077503386.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647260.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..130
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..130
                     /product="peptide methionine sulfoxide reductase-like"
                     /calculated_mol_wt=14788
     Region          <1..75
                     /region_name="PMSR"
                     /note="Peptide methionine sulfoxide reductase; pfam01625"
                     /db_xref="CDD:460270"
     CDS             1..130
                     /gene="LOC144113882"
                     /coded_by="XM_077647260.1:368..760"
                     /db_xref="GeneID:144113882"
ORIGIN      
        1 mfwdfhdpta chkrqymsai fyhdkeqkai aeeslkrqqs kikkpiatki mpagtfydae
       61 dyhqkyllrr htalceslkk aglknfkesh vaarlngycs gsgtlaafea ereklglsdt
      121 qaalvrqcfk
//
LOCUS       XP_077503387             531 aa            linear   INV 25-NOV-2025
DEFINITION  protein O-glucosyltransferase 2-like [Amblyomma americanum].
ACCESSION   XP_077503387
VERSION     XP_077503387.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647261.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..531
                     /product="protein O-glucosyltransferase 2-like"
                     /calculated_mol_wt=60817
     Region          64..154
                     /region_name="Filamin"
                     /note="Filamin/ABP280 repeat; pfam00630"
                     /db_xref="CDD:395505"
     Region          180..521
                     /region_name="Glyco_transf_90"
                     /note="Glycosyl transferase family 90; cl23835"
                     /db_xref="CDD:329112"
     CDS             1..531
                     /gene="LOC144113883"
                     /coded_by="XM_077647261.1:484..2079"
                     /db_xref="GeneID:144113883"
ORIGIN      
        1 mgkrhdsrfr dvtfshcrml lagqlcaail lvavvaakdq crlrsacger saassrsepk
       61 vsarvygpgl rgcfnlptry ffvhvvdasg nnfttasakn ltvtiagtkg vcsiwtqvls
      121 rgdglfivry klfktcpeak levkfngspl mesplllkgp lyndacscpa pfedwllqte
      181 cptvdpqisk dlsqfeeidm rkmlrealrr fdrpgsvsfc hyaviknkvy rkcygqhvgf
      241 nmfmdqillf larkvllpdv emlvnlgdwp lerksysgvh ipffswcgse dstdivmpty
      301 dltesslemm grvtldllsv qgnsgpvwke kqscgfwrgr dsrqerldlv alsrrhpell
      361 nasltnffff rdkmdiygpq ashvsffdff eykyqinvdg tvaayrlpyl lagsslvlkq
      421 dskyyehfys rlipmvhyvp fqrnlsdlie klvwahehdg taqrivkeaq qfaldhllph
      481 hvfcyyaqll qqysfklkrr akvaegmeev lqprdnqpcd crqqqrnhde l
//
LOCUS       XP_077503388             291 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113884 [Amblyomma americanum].
ACCESSION   XP_077503388
VERSION     XP_077503388.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647262.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..291
                     /product="uncharacterized protein LOC144113884"
                     /calculated_mol_wt=32744
     CDS             1..291
                     /gene="LOC144113884"
                     /coded_by="XM_077647262.1:126..1001"
                     /db_xref="GeneID:144113884"
ORIGIN      
        1 mplsrlssas rrrsrsatms vhyklcwhee ivykfklewp pdsadilhhe qgiiesivqm
       61 sregqcpssl staefavniq lsewklsgli ffekdgvtye pyhhqfllri qkkhvdlppt
      121 aqmapsvsss eglstdsslp tpleqeppls dearrkirlp ahnrqkklsd traalrgqsk
      181 rdllrttpye vqssfrqvrp sysrrkltis rynipdpiyr ailqavnase qvqdgtiiil
      241 dntilrdgmg praidqfgrc lapastegsg ilhtlkrcvs gvlgkisnwi s
//
LOCUS       XP_077503389             291 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113885 [Amblyomma americanum].
ACCESSION   XP_077503389
VERSION     XP_077503389.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647263.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..291
                     /product="uncharacterized protein LOC144113885"
                     /calculated_mol_wt=32744
     CDS             1..291
                     /gene="LOC144113885"
                     /coded_by="XM_077647263.1:126..1001"
                     /db_xref="GeneID:144113885"
ORIGIN      
        1 mplsrlssas rrrsrsatms vhyklcwhee ivykfklewp pdsadilhhe qgiiesivqm
       61 sregqcpssl staefavniq lsewklsgli ffekdgvtye pyhhqfllri qkkhvdlppt
      121 aqmapsvsss eglstdsslp tpleqeppls dearrkirlp ahnrqkklsd traalrgqsk
      181 rdllrttpye vqssfrqvrp sysrrkltis rynipdpiyr ailqavnase qvqdgtiiil
      241 dntilrdgmg praidqfgrc lapastegsg ilhtlkrcvs gvlgkisnwi s
//
LOCUS       XP_077503390             291 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113887 [Amblyomma americanum].
ACCESSION   XP_077503390
VERSION     XP_077503390.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647264.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..291
                     /product="uncharacterized protein LOC144113887"
                     /calculated_mol_wt=32744
     CDS             1..291
                     /gene="LOC144113887"
                     /coded_by="XM_077647264.1:126..1001"
                     /db_xref="GeneID:144113887"
ORIGIN      
        1 mplsrlssas rrrsrsatms vhyklcwhee ivykfklewp pdsadilhhe qgiiesivqm
       61 sregqcpssl staefavniq lsewklsgli ffekdgvtye pyhhqfllri qkkhvdlppt
      121 aqmapsvsss eglstdsslp tpleqeppls dearrkirlp ahnrqkklsd traalrgqsk
      181 rdllrttpye vqssfrqvrp sysrrkltis rynipdpiyr ailqavnase qvqdgtiiil
      241 dntilrdgmg praidqfgrc lapastegsg ilhtlkrcvs gvlgkisnwi s
//
LOCUS       XP_077503391             273 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113888 [Amblyomma americanum].
ACCESSION   XP_077503391
VERSION     XP_077503391.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647265.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..273
                     /product="uncharacterized protein LOC144113888"
                     /calculated_mol_wt=30557
     CDS             1..273
                     /gene="LOC144113888"
                     /coded_by="XM_077647265.1:70..891"
                     /db_xref="GeneID:144113888"
ORIGIN      
        1 msvhyklcwh eeivykfkle wppdsadilh heqgiiesiv qmsregqcps slstaefavn
       61 iqlsewklsg liffekdgvt yepyhhqfll riqkkhvdlp ptaqvtpsvs sseglstdss
      121 lptpleqepp lsdearrkig lpahnrqkkl sdtraalrgq skrdllrttp yevqssfrqv
      181 rpsysrrklt isrynipdpi yrailqavna seqvqdgtii ildntilgdg mgpraidqfg
      241 rclapasteg sgilhtlkrc isgvlgkisn wis
//
LOCUS       XP_077503392             273 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113889 [Amblyomma americanum].
ACCESSION   XP_077503392
VERSION     XP_077503392.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647266.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..273
                     /product="uncharacterized protein LOC144113889"
                     /calculated_mol_wt=30736
     CDS             1..273
                     /gene="LOC144113889"
                     /coded_by="XM_077647266.1:105..926"
                     /db_xref="GeneID:144113889"
ORIGIN      
        1 msvhyklcwh eeivykfkle wppdsadilh heqgiiesiv qmsregqcps sfstaefavn
       61 iqltewklsg liffekdgvt yepyhhqfll riqkkhvdlp ptaqmtpsvs sseglstdss
      121 lptpleqepp lsdearrkir lpahnrqkkl sdtraalrgq skrdllrttp yevqssfrqv
      181 rpsysrrklt isrynipdpi yrailqavna seqvqdgtii ildntilgdg mgpraidqfg
      241 rclapasteg sgilhtlkrc isgvlgkisn wis
//
LOCUS       XP_077503394             235 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113890 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503394
VERSION     XP_077503394.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647268.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..235
                     /product="uncharacterized protein LOC144113890 isoform X1"
                     /calculated_mol_wt=26032
     CDS             1..235
                     /gene="LOC144113890"
                     /coded_by="XM_077647268.1:73..780"
                     /db_xref="GeneID:144113890"
ORIGIN      
        1 msrflkhskv fddskgrffl vllteasder lsklslsitd gnsvwkaats feescgslks
       61 rreenvkhlm krisncrles iddaeeltvt lvppgktyel kfkksadgsa qnsstsvlne
      121 vdynkdvvsl wcrpgikdth afcilrniti scaqhgssav krhanmtkhl qaaakkrdsn
      181 gvllapktvq ntidfsqgsh vphcrikcak lshicnvccl kehsllvgrh snlhl
//
LOCUS       XP_077503395             178 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113890 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503395
VERSION     XP_077503395.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647269.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..178
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..178
                     /product="uncharacterized protein LOC144113890 isoform X2"
                     /calculated_mol_wt=19783
     CDS             1..178
                     /gene="LOC144113890"
                     /coded_by="XM_077647269.1:66..602"
                     /db_xref="GeneID:144113890"
ORIGIN      
        1 msrflkhskv fddskgrffl vllteasder lsklslsitd gnsvwkaats feescgslks
       61 rreenvkhlm krisncrles iddaeeltvt lvppgktyel kfkksadvsa alqrfdllva
      121 vaeagdlktl vdtvdedila vspskraksr kkeavpveke gmnllnprsr kiahercs
//
LOCUS       XP_077503396             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113890 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503396
VERSION     XP_077503396.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647270.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144113890 isoform X3"
                     /calculated_mol_wt=17933
     CDS             1..158
                     /gene="LOC144113890"
                     /coded_by="XM_077647270.1:65..541"
                     /db_xref="GeneID:144113890"
ORIGIN      
        1 msrflkhskv fddskgrffl vllteasder lsklslsitd gnsvwkaats feescgslks
       61 rreenvkhlm krisncrles iddaeeltvt lvppgktyel kfkksadhsv ifylvillhn
      121 vdskvpslvp lfqntmprld alkmvyrgih dtswslqq
//
LOCUS       XP_077503397             146 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113890 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503397
VERSION     XP_077503397.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647271.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..146
                     /product="uncharacterized protein LOC144113890 isoform X4"
                     /calculated_mol_wt=16054
     CDS             1..146
                     /gene="LOC144113890"
                     /coded_by="XM_077647271.1:67..507"
                     /db_xref="GeneID:144113890"
ORIGIN      
        1 msrflkhskv fddskgrffl vllteasder lsklslsitd gnsvwkaats feescgslks
       61 rreenvkhlm krisncrles iddaeeltvt lvppgktyel kfkksadars algvqavqrn
      121 rkqasngsca lsaaipqqft atsrpt
//
LOCUS       XP_077503398             235 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113891 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503398
VERSION     XP_077503398.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647272.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..235
                     /product="uncharacterized protein LOC144113891 isoform X1"
                     /calculated_mol_wt=26020
     CDS             1..235
                     /gene="LOC144113891"
                     /coded_by="XM_077647272.1:116..823"
                     /db_xref="GeneID:144113891"
ORIGIN      
        1 msrflkhskv fddskgrffl vllteasdar lsklslsitd gnsvwkaats feescgslks
       61 rreenvkhlm krisncrles iddaeeltvt lvppgktyel kfkksadgsa qnsstsvlne
      121 vdynkdvvsl wcrpgikdth afcilrniti scaqhcssav krhanmtkhl qaaakkrdsn
      181 gvllapktvq ntidfsqgsh vphcrikcak lshicnvccl kehsllvgrh snlhl
//
LOCUS       XP_077503399             235 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113891 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503399
VERSION     XP_077503399.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647273.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..235
                     /product="uncharacterized protein LOC144113891 isoform X1"
                     /calculated_mol_wt=26020
     CDS             1..235
                     /gene="LOC144113891"
                     /coded_by="XM_077647273.1:116..823"
                     /db_xref="GeneID:144113891"
ORIGIN      
        1 msrflkhskv fddskgrffl vllteasdar lsklslsitd gnsvwkaats feescgslks
       61 rreenvkhlm krisncrles iddaeeltvt lvppgktyel kfkksadgsa qnsstsvlne
      121 vdynkdvvsl wcrpgikdth afcilrniti scaqhcssav krhanmtkhl qaaakkrdsn
      181 gvllapktvq ntidfsqgsh vphcrikcak lshicnvccl kehsllvgrh snlhl
//
LOCUS       XP_077503400             178 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113891 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503400
VERSION     XP_077503400.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647274.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..178
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..178
                     /product="uncharacterized protein LOC144113891 isoform X2"
                     /calculated_mol_wt=19738
     CDS             1..178
                     /gene="LOC144113891"
                     /coded_by="XM_077647274.1:116..652"
                     /db_xref="GeneID:144113891"
ORIGIN      
        1 msrflkhskv fddskgrffl vllteasdar lsklslsitd gnsvwkaats feescgslks
       61 rreenvkhlm krisncrles iddaeeltvt lvppgktyel kfkksadvsa alqrfdllva
      121 vaeagdlktl vdtvdedila vspskraksr kkeavhveke gmsllnprsr kiahercs
//
LOCUS       XP_077503401             146 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113891 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503401
VERSION     XP_077503401.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647275.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..146
                     /product="uncharacterized protein LOC144113891 isoform X3"
                     /calculated_mol_wt=15996
     CDS             1..146
                     /gene="LOC144113891"
                     /coded_by="XM_077647275.1:116..556"
                     /db_xref="GeneID:144113891"
ORIGIN      
        1 msrflkhskv fddskgrffl vllteasdar lsklslsitd gnsvwkaats feescgslks
       61 rreenvkhlm krisncrles iddaeeltvt lvppgktyel kfkksadars algvqavqrn
      121 rkqasngsca lsaaipqqft atsrpt
//
LOCUS       XP_077503402             218 aa            linear   INV 25-NOV-2025
DEFINITION  peptide deformylase, mitochondrial-like [Amblyomma americanum].
ACCESSION   XP_077503402
VERSION     XP_077503402.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647276.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..218
                     /product="peptide deformylase, mitochondrial-like"
                     /calculated_mol_wt=25119
     Region          35..199
                     /region_name="Pep_deformylase"
                     /note="Polypeptide deformylase; pfam01327"
                     /db_xref="CDD:426202"
     Site            order(76..78,83,139..141,182..183,186)
                     /site_type="active"
                     /db_xref="CDD:238271"
     Site            order(78,83,141,183)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:238271"
     Site            order(140,182,186)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:238271"
     CDS             1..218
                     /gene="LOC144113892"
                     /coded_by="XM_077647276.1:130..786"
                     /db_xref="GeneID:144113892"
ORIGIN      
        1 mkmmfkkllt scfryvsklk dygvttnptp pyefmcqvgd pvlraraepv dpvkvtsaev
       61 qkiiavmknv mvnshsvgis apqvgcplri immefpskym klaekdeakt rlyqvfplki
      121 finpimeitn sqkivlpegc esvrgftadv pryyevkvsg lnqygevhew qthgwparii
      181 qhemdhlegs lfidfmdprt fnfnywhlik klpksrlr
//
LOCUS       XP_077503403             203 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113894 [Amblyomma americanum].
ACCESSION   XP_077503403
VERSION     XP_077503403.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647277.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..203
                     /product="uncharacterized protein LOC144113894"
                     /calculated_mol_wt=22309
     Region          1..>135
                     /region_name="MtnA"
                     /note="5-methylthioribose/5-deoxyribulose 1-phosphate
                     isomerase (methionine salvage pathway), a paralog of
                     eIF-2B alpha subunit [Amino acid transport and
                     metabolism]; COG0182"
                     /db_xref="CDD:439952"
     CDS             1..203
                     /gene="LOC144113894"
                     /coded_by="XM_077647277.1:863..1474"
                     /db_xref="GeneID:144113894"
ORIGIN      
        1 mlqairyqag tleildqwql pheshylmla dakavwevih smkvqgapai amvaalglav
       61 eltrsqlpss radlaalvhk wldylstsrp tavnlanmad hlgckadtla kneglsveam
      121 rdaicaeaed mlaadwpptk placteqstw wptvgqrlat anwacspiat rapwprrvta
      181 lhsvrrprrc cqrkcqslda lft
//
LOCUS       XP_077503404             178 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113895 [Amblyomma americanum].
ACCESSION   XP_077503404
VERSION     XP_077503404.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647278.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..178
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..178
                     /product="uncharacterized protein LOC144113895"
                     /calculated_mol_wt=19783
     CDS             1..178
                     /gene="LOC144113895"
                     /coded_by="XM_077647278.1:65..601"
                     /db_xref="GeneID:144113895"
ORIGIN      
        1 msrflkhskv fddskgrffl vllteasder lsklslsitd gnsvwkaats feescgslks
       61 rreenvkhlm krisncrles iddaeeltvt lvppgktyel kfkksadvsa alqrfdllva
      121 vaeagdlktl vdtvdedila vspskraksr kkeavpveke gmnllnprsr kiahercs
//
LOCUS       XP_077503406             128 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113896 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503406
VERSION     XP_077503406.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647280.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..128
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..128
                     /product="uncharacterized protein LOC144113896 isoform X1"
                     /calculated_mol_wt=13341
     CDS             1..128
                     /gene="LOC144113896"
                     /coded_by="XM_077647280.1:1512..1898"
                     /db_xref="GeneID:144113896"
ORIGIN      
        1 mgvlvarfad dddilnrkag vavlqqgkhr grrhlgshih vgrglksgtg vrsrlpwsss
       61 pgdrpssvgl alsiplttes saatgfsgps tvsplasfrs ttprclvvrs rrpgqhisqg
      121 hlfagnka
//
LOCUS       XP_077503407              62 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113896 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503407
VERSION     XP_077503407.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647281.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..62
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..62
                     /product="uncharacterized protein LOC144113896 isoform X2"
                     /calculated_mol_wt=6336
     CDS             1..62
                     /gene="LOC144113896"
                     /coded_by="XM_077647281.1:135..323"
                     /db_xref="GeneID:144113896"
ORIGIN      
        1 mhgstksipl ttessaatgf sgpstvspla sfrsttprcl vvrsrrpgqh isqghlfagn
       61 ka
//
LOCUS       XP_077503408             113 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113897 [Amblyomma americanum].
ACCESSION   XP_077503408
VERSION     XP_077503408.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647282.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..113
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..113
                     /product="uncharacterized protein LOC144113897"
                     /calculated_mol_wt=12810
     CDS             1..113
                     /gene="LOC144113897"
                     /coded_by="XM_077647282.1:130..471"
                     /db_xref="GeneID:144113897"
ORIGIN      
        1 mkmmfkkllt scfryvsklk dygvttnptp pyefmcqvgd pvlraraepv dpvkvtsaev
       61 qkhsvifylv illhnvdskv pslvpllqnt mprldalkmv yrgihdtsws lqq
//
LOCUS       XP_077503409             113 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113898 [Amblyomma americanum].
ACCESSION   XP_077503409
VERSION     XP_077503409.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647283.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..113
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..113
                     /product="uncharacterized protein LOC144113898"
                     /calculated_mol_wt=12810
     CDS             1..113
                     /gene="LOC144113898"
                     /coded_by="XM_077647283.1:130..471"
                     /db_xref="GeneID:144113898"
ORIGIN      
        1 mkmmfkkllt scfryvsklk dygvttnptp pyefmcqvgd pvlraraepv dpvkvtsaev
       61 qkhsvifylv illhnvdskv pslvpllqnt mprldalkmv yrgihdtsws lqq
//
LOCUS       XP_077503410              87 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylinositol N-acetylglucosaminyltransferase subunit H-like
            [Amblyomma americanum].
ACCESSION   XP_077503410
VERSION     XP_077503410.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647284.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..87
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..87
                     /product="phosphatidylinositol
                     N-acetylglucosaminyltransferase subunit H-like"
                     /calculated_mol_wt=9934
     Region          <14..62
                     /region_name="PIG-H"
                     /note="GPI-GlcNAc transferase complex, PIG-H component;
                     pfam10181"
                     /db_xref="CDD:462983"
     CDS             1..87
                     /gene="LOC144113899"
                     /coded_by="XM_077647284.1:27..290"
                     /db_xref="GeneID:144113899"
ORIGIN      
        1 mgvlvarfad dddrqesqff nreniedvvi seaismhsvi fylvillhnv dskvpsllpl
       61 fqntmprlda lkmvyrgihd tswslqq
//
LOCUS       XP_077503411             164 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113900 [Amblyomma americanum].
ACCESSION   XP_077503411
VERSION     XP_077503411.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647285.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..164
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..164
                     /product="uncharacterized protein LOC144113900"
                     /calculated_mol_wt=18808
     Region          2..22
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(2,5,18,22)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          <5..146
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          30..50
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(30,33,46,50)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(35,37,39,41..42,45..46,49,63,65,69..70,73..74,77,91,
                     93,95,97..98,101..102,105)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          58..78
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          86..106
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          114..134
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          142..162
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..164
                     /gene="LOC144113900"
                     /coded_by="XM_077647285.1:231..725"
                     /db_xref="GeneID:144113900"
ORIGIN      
        1 mcvhcsksfi rkasmishir ihtgekpfqc trcsksftdq snmakhirth tgdkpfqcph
       61 csksfnvkfn lvthirthtg ekpfqcthcs ksfterssma khirthtgdk pfqcphcsks
      121 ftqkmnmvth vrthtgerpy qcnlcpeafa qkghlerhak thkk
//
LOCUS       XP_077503412            1280 aa            linear   INV 25-NOV-2025
DEFINITION  enhancer of mRNA-decapping protein 4 homolog Ge-1 isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077503412
VERSION     XP_077503412.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647286.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1280
                     /product="enhancer of mRNA-decapping protein 4 homolog
                     Ge-1 isoform X1"
                     /calculated_mol_wt=138939
     Region          113..441
                     /region_name="Ge1_WD40"
                     /note="WD40 region of Ge1, enhancer of mRNA-decapping
                     protein; pfam16529"
                     /db_xref="CDD:465162"
     Region          303..346
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          351..398
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <602..700
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..1280
                     /gene="Ge-1"
                     /coded_by="XM_077647286.1:96..3938"
                     /db_xref="GeneID:144113906"
ORIGIN      
        1 msshegsida neatrllkdi lhlgkspsap segveanggl deaaggggdp tdgmaaggnl
       61 lesifrssqe nqqeiqqsqq qiclldneed vsvdvcgkev qvvvsrmhgn assnrvkikn
      121 vvdyswelky nygkhiclhm sgaylayalr aanssagvvr vlnlktgqrl lvkgfrgpiq
      181 dmafawlatt vmlavvdvyg tlcvysidnd tsstllfkve rppdevpsey rrvvwcpyvp
      241 ddgsrggnsa eddnskvlva tnleeaevwn vgcivaeygm rtltvpevri glqriiehtk
      301 piidaafapd gtalatagad gqvrffqvym qepdasprcl hqwcphnekp vtclffldnm
      361 kacnpetqfw kyaltgtdhn telklwscet waclqtirfi vspvdrdllp afkveidpta
      421 cyvvlsdvhr kvvyvgqlql dnkaelvsla lfpvtlpvls favveavrcr ykpstdtehv
      481 drldaeseea dsadeertfa ktqegtmvrl ywmttksiqa chivypltas salssavslg
      541 tlsqnstvsg ctdhlsdvsa dadddekagq nkhdadvpsq kgkvhgddds sgfaegkvll
      601 kpsdfvspsn sstafnpcta aastntdplp pttspcvrnt stpvpgelcc npmpgslasp
      661 gaasgvldls rkgvggdaav akaattngdc elsdppqrkl sqrstassss qevadimaps
      721 emlanagnst deeeedddel dptldydsaa dethpgetea aahepslltp pasegqevgd
      781 agwprppekt egfhvapttt tvcdqevgad pleqdqnsah alsrlellis qhleennalt
      841 qtvgtlssql redhaqlqkl qewqshekdl vssllaecka nrtsfarlen aiskltelqk
      901 vgvekqstmv aqsldamlgi nlekfianef kitvvpsivk iidltrceis grletteali
      961 kestikavks kamfdavaqa tstsiqshve ttcrdilenv lmpsldrlch slfsqlgeif
     1021 qkgtreflhq aqehidkhnq eqvaeamkkv qsdverslqt flrdhqaalv ppdfrapfde
     1081 avnnalqgle tgllqaltgq qeklmnslke emhgvlkesv aaavaegaas rskmatpvpf
     1141 adphlllqqi tllmrqgqyn mafqqalsva dlatvvstce mvsptsifgq hpcplqqpvl
     1201 lsliqqlcad lsssseiklk yleeavlsld kenaitkehm kvilsqlclk lnnfimgtas
     1261 hdlgrmarrl lmvtqsllns
//
LOCUS       XP_077503413            1258 aa            linear   INV 25-NOV-2025
DEFINITION  enhancer of mRNA-decapping protein 4 homolog Ge-1 isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077503413
VERSION     XP_077503413.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647287.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1258
                     /product="enhancer of mRNA-decapping protein 4 homolog
                     Ge-1 isoform X2"
                     /calculated_mol_wt=136805
     Region          91..419
                     /region_name="Ge1_WD40"
                     /note="WD40 region of Ge1, enhancer of mRNA-decapping
                     protein; pfam16529"
                     /db_xref="CDD:465162"
     Region          281..324
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          329..376
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <580..678
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..1258
                     /gene="Ge-1"
                     /coded_by="XM_077647287.1:130..3906"
                     /db_xref="GeneID:144113906"
ORIGIN      
        1 mqrspypyse gveangglde aaggggdptd gmaaggnlle sifrssqenq qeiqqsqqqi
       61 clldneedvs vdvcgkevqv vvsrmhgnas snrvkiknvv dyswelkyny gkhiclhmsg
      121 aylayalraa nssagvvrvl nlktgqrllv kgfrgpiqdm afawlattvm lavvdvygtl
      181 cvysidndts stllfkverp pdevpseyrr vvwcpyvpdd gsrggnsaed dnskvlvatn
      241 leeaevwnvg civaeygmrt ltvpevrigl qriiehtkpi idaafapdgt alatagadgq
      301 vrffqvymqe pdasprclhq wcphnekpvt clffldnmka cnpetqfwky altgtdhnte
      361 lklwscetwa clqtirfivs pvdrdllpaf kveidptacy vvlsdvhrkv vyvgqlqldn
      421 kaelvslalf pvtlpvlsfa vveavrcryk pstdtehvdr ldaeseeads adeertfakt
      481 qegtmvrlyw mttksiqach ivypltassa lssavslgtl sqnstvsgct dhlsdvsada
      541 dddekagqnk hdadvpsqkg kvhgdddssg faegkvllkp sdfvspsnss tafnpctaaa
      601 stntdplppt tspcvrntst pvpgelccnp mpgslaspga asgvldlsrk gvggdaavak
      661 aattngdcel sdppqrklsq rstassssqe vadimapsem lanagnstde eeedddeldp
      721 tldydsaade thpgeteaaa hepslltppa segqevgdag wprppekteg fhvapttttv
      781 cdqevgadpl eqdqnsahal srlellisqh leennaltqt vgtlssqlre dhaqlqklqe
      841 wqshekdlvs sllaeckanr tsfarlenai skltelqkvg vekqstmvaq sldamlginl
      901 ekfianefki tvvpsivkii dltrceisgr lettealike stikavkska mfdavaqats
      961 tsiqshvett crdilenvlm psldrlchsl fsqlgeifqk gtreflhqaq ehidkhnqeq
     1021 vaeamkkvqs dverslqtfl rdhqaalvpp dfrapfdeav nnalqgletg llqaltgqqe
     1081 klmnslkeem hgvlkesvaa avaegaasrs kmatpvpfad phlllqqitl lmrqgqynma
     1141 fqqalsvadl atvvstcemv sptsifgqhp cplqqpvlls liqqlcadls ssseiklkyl
     1201 eeavlsldke naitkehmkv ilsqlclkln nfimgtashd lgrmarrllm vtqsllns
//
LOCUS       XP_077503414            1256 aa            linear   INV 25-NOV-2025
DEFINITION  enhancer of mRNA-decapping protein 4 homolog Ge-1 isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077503414
VERSION     XP_077503414.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647288.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1256
                     /product="enhancer of mRNA-decapping protein 4 homolog
                     Ge-1 isoform X3"
                     /calculated_mol_wt=136312
     Region          113..441
                     /region_name="Ge1_WD40"
                     /note="WD40 region of Ge1, enhancer of mRNA-decapping
                     protein; pfam16529"
                     /db_xref="CDD:465162"
     Region          303..346
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          351..398
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <602..841
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..1256
                     /gene="Ge-1"
                     /coded_by="XM_077647288.1:96..3866"
                     /db_xref="GeneID:144113906"
ORIGIN      
        1 msshegsida neatrllkdi lhlgkspsap segveanggl deaaggggdp tdgmaaggnl
       61 lesifrssqe nqqeiqqsqq qiclldneed vsvdvcgkev qvvvsrmhgn assnrvkikn
      121 vvdyswelky nygkhiclhm sgaylayalr aanssagvvr vlnlktgqrl lvkgfrgpiq
      181 dmafawlatt vmlavvdvyg tlcvysidnd tsstllfkve rppdevpsey rrvvwcpyvp
      241 ddgsrggnsa eddnskvlva tnleeaevwn vgcivaeygm rtltvpevri glqriiehtk
      301 piidaafapd gtalatagad gqvrffqvym qepdasprcl hqwcphnekp vtclffldnm
      361 kacnpetqfw kyaltgtdhn telklwscet waclqtirfi vspvdrdllp afkveidpta
      421 cyvvlsdvhr kvvyvgqlql dnkaelvsla lfpvtlpvls favveavrcr ykpstdtehv
      481 drldaeseea dsadeertfa ktqegtmvrl ywmttksiqa chivypltas salssavslg
      541 tlsqnstvsg ctdhlsdvsa dadddekagq nkhdadvpsq kgkvhgddds sgfaegkvll
      601 kpsdfvspsn sstafnpcta aastntdplp pttspcvrnt stpvpgelcc npmpgslasp
      661 gaasgvldls rkgvggdaav akaattngdc elsdppqrkl sqrstassss qevadimaps
      721 emlanadeth pgeteaaahe pslltppase gqevgdagwp rppektegfh vapttttvcd
      781 qevgadpleq dqnsahalsr lellisqhle ennaltqtvg tlssqlredh aqlqklqewq
      841 shekdlvssl laeckanrts farlenaisk ltelqkvgve kqstmvaqsl damlginlek
      901 fianefkitv vpsivkiidl trceisgrle ttealikest ikavkskamf davaqatsts
      961 iqshvettcr dilenvlmps ldrlchslfs qlgeifqkgt reflhqaqeh idkhnqeqva
     1021 eamkkvqsdv erslqtflrd hqaalvppdf rapfdeavnn alqgletgll qaltgqqekl
     1081 mnslkeemhg vlkesvaaav aegaasrskm atpvpfadph lllqqitllm rqgqynmafq
     1141 qalsvadlat vvstcemvsp tsifgqhpcp lqqpvllsli qqlcadlsss seiklkylee
     1201 avlsldkena itkehmkvil sqlclklnnf imgtashdlg rmarrllmvt qsllns
//
LOCUS       XP_077503415            1234 aa            linear   INV 25-NOV-2025
DEFINITION  enhancer of mRNA-decapping protein 4 homolog Ge-1 isoform X4
            [Amblyomma americanum].
ACCESSION   XP_077503415
VERSION     XP_077503415.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647289.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1234
                     /product="enhancer of mRNA-decapping protein 4 homolog
                     Ge-1 isoform X4"
                     /calculated_mol_wt=134177
     Region          91..419
                     /region_name="Ge1_WD40"
                     /note="WD40 region of Ge1, enhancer of mRNA-decapping
                     protein; pfam16529"
                     /db_xref="CDD:465162"
     Region          281..324
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          329..376
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <580..819
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..1234
                     /gene="Ge-1"
                     /coded_by="XM_077647289.1:175..3879"
                     /db_xref="GeneID:144113906"
ORIGIN      
        1 mqrspypyse gveangglde aaggggdptd gmaaggnlle sifrssqenq qeiqqsqqqi
       61 clldneedvs vdvcgkevqv vvsrmhgnas snrvkiknvv dyswelkyny gkhiclhmsg
      121 aylayalraa nssagvvrvl nlktgqrllv kgfrgpiqdm afawlattvm lavvdvygtl
      181 cvysidndts stllfkverp pdevpseyrr vvwcpyvpdd gsrggnsaed dnskvlvatn
      241 leeaevwnvg civaeygmrt ltvpevrigl qriiehtkpi idaafapdgt alatagadgq
      301 vrffqvymqe pdasprclhq wcphnekpvt clffldnmka cnpetqfwky altgtdhnte
      361 lklwscetwa clqtirfivs pvdrdllpaf kveidptacy vvlsdvhrkv vyvgqlqldn
      421 kaelvslalf pvtlpvlsfa vveavrcryk pstdtehvdr ldaeseeads adeertfakt
      481 qegtmvrlyw mttksiqach ivypltassa lssavslgtl sqnstvsgct dhlsdvsada
      541 dddekagqnk hdadvpsqkg kvhgdddssg faegkvllkp sdfvspsnss tafnpctaaa
      601 stntdplppt tspcvrntst pvpgelccnp mpgslaspga asgvldlsrk gvggdaavak
      661 aattngdcel sdppqrklsq rstassssqe vadimapsem lanadethpg eteaaaheps
      721 lltppasegq evgdagwprp pektegfhva pttttvcdqe vgadpleqdq nsahalsrle
      781 llisqhleen naltqtvgtl ssqlredhaq lqklqewqsh ekdlvsslla eckanrtsfa
      841 rlenaisklt elqkvgvekq stmvaqslda mlginlekfi anefkitvvp sivkiidltr
      901 ceisgrlett ealikestik avkskamfda vaqatstsiq shvettcrdi lenvlmpsld
      961 rlchslfsql geifqkgtre flhqaqehid khnqeqvaea mkkvqsdver slqtflrdhq
     1021 aalvppdfra pfdeavnnal qgletgllqa ltgqqeklmn slkeemhgvl kesvaaavae
     1081 gaasrskmat pvpfadphll lqqitllmrq gqynmafqqa lsvadlatvv stcemvspts
     1141 ifgqhpcplq qpvllsliqq lcadlsssse iklkyleeav lsldkenait kehmkvilsq
     1201 lclklnnfim gtashdlgrm arrllmvtqs llns
//
LOCUS       XP_077503416            1200 aa            linear   INV 25-NOV-2025
DEFINITION  enhancer of mRNA-decapping protein 4 homolog Ge-1 isoform X5
            [Amblyomma americanum].
ACCESSION   XP_077503416
VERSION     XP_077503416.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647290.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1200
                     /product="enhancer of mRNA-decapping protein 4 homolog
                     Ge-1 isoform X5"
                     /calculated_mol_wt=131022
     Region          113..441
                     /region_name="Ge1_WD40"
                     /note="WD40 region of Ge1, enhancer of mRNA-decapping
                     protein; pfam16529"
                     /db_xref="CDD:465162"
     Region          303..346
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          351..398
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..1200
                     /gene="Ge-1"
                     /coded_by="XM_077647290.1:96..3698"
                     /db_xref="GeneID:144113906"
ORIGIN      
        1 msshegsida neatrllkdi lhlgkspsap segveanggl deaaggggdp tdgmaaggnl
       61 lesifrssqe nqqeiqqsqq qiclldneed vsvdvcgkev qvvvsrmhgn assnrvkikn
      121 vvdyswelky nygkhiclhm sgaylayalr aanssagvvr vlnlktgqrl lvkgfrgpiq
      181 dmafawlatt vmlavvdvyg tlcvysidnd tsstllfkve rppdevpsey rrvvwcpyvp
      241 ddgsrggnsa eddnskvlva tnleeaevwn vgcivaeygm rtltvpevri glqriiehtk
      301 piidaafapd gtalatagad gqvrffqvym qepdasprcl hqwcphnekp vtclffldnm
      361 kacnpetqfw kyaltgtdhn telklwscet waclqtirfi vspvdrdllp afkveidpta
      421 cyvvlsdvhr kvvyvgqlql dnkaelvsla lfpvtlpvls favveavrcr ykpstdtehv
      481 drldaeseea dsadeertfa ktqegtmvrl ywmttksiqa chivypltas salssavslg
      541 tlsqnstvsg ctdhlsdvsa dadddekagq nkhdadvpsq kgkvhgddds sgfaegkvll
      601 kpsdfvspsn sstafnpcta aastntdplp pttspcvrnt stpvpagnst deeeedddel
      661 dptldydsaa dethpgetea aahepslltp pasegqevgd agwprppekt egfhvapttt
      721 tvcdqevgad pleqdqnsah alsrlellis qhleennalt qtvgtlssql redhaqlqkl
      781 qewqshekdl vssllaecka nrtsfarlen aiskltelqk vgvekqstmv aqsldamlgi
      841 nlekfianef kitvvpsivk iidltrceis grletteali kestikavks kamfdavaqa
      901 tstsiqshve ttcrdilenv lmpsldrlch slfsqlgeif qkgtreflhq aqehidkhnq
      961 eqvaeamkkv qsdverslqt flrdhqaalv ppdfrapfde avnnalqgle tgllqaltgq
     1021 qeklmnslke emhgvlkesv aaavaegaas rskmatpvpf adphlllqqi tllmrqgqyn
     1081 mafqqalsva dlatvvstce mvsptsifgq hpcplqqpvl lsliqqlcad lsssseiklk
     1141 yleeavlsld kenaitkehm kvilsqlclk lnnfimgtas hdlgrmarrl lmvtqsllns
//
LOCUS       XP_077503417            1178 aa            linear   INV 25-NOV-2025
DEFINITION  enhancer of mRNA-decapping protein 4 homolog Ge-1 isoform X6
            [Amblyomma americanum].
ACCESSION   XP_077503417
VERSION     XP_077503417.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647291.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1178
                     /product="enhancer of mRNA-decapping protein 4 homolog
                     Ge-1 isoform X6"
                     /calculated_mol_wt=128888
     Region          91..419
                     /region_name="Ge1_WD40"
                     /note="WD40 region of Ge1, enhancer of mRNA-decapping
                     protein; pfam16529"
                     /db_xref="CDD:465162"
     Region          281..324
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          329..376
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..1178
                     /gene="Ge-1"
                     /coded_by="XM_077647291.1:123..3659"
                     /db_xref="GeneID:144113906"
ORIGIN      
        1 mqrspypyse gveangglde aaggggdptd gmaaggnlle sifrssqenq qeiqqsqqqi
       61 clldneedvs vdvcgkevqv vvsrmhgnas snrvkiknvv dyswelkyny gkhiclhmsg
      121 aylayalraa nssagvvrvl nlktgqrllv kgfrgpiqdm afawlattvm lavvdvygtl
      181 cvysidndts stllfkverp pdevpseyrr vvwcpyvpdd gsrggnsaed dnskvlvatn
      241 leeaevwnvg civaeygmrt ltvpevrigl qriiehtkpi idaafapdgt alatagadgq
      301 vrffqvymqe pdasprclhq wcphnekpvt clffldnmka cnpetqfwky altgtdhnte
      361 lklwscetwa clqtirfivs pvdrdllpaf kveidptacy vvlsdvhrkv vyvgqlqldn
      421 kaelvslalf pvtlpvlsfa vveavrcryk pstdtehvdr ldaeseeads adeertfakt
      481 qegtmvrlyw mttksiqach ivypltassa lssavslgtl sqnstvsgct dhlsdvsada
      541 dddekagqnk hdadvpsqkg kvhgdddssg faegkvllkp sdfvspsnss tafnpctaaa
      601 stntdplppt tspcvrntst pvpagnstde eeedddeldp tldydsaade thpgeteaaa
      661 hepslltppa segqevgdag wprppekteg fhvapttttv cdqevgadpl eqdqnsahal
      721 srlellisqh leennaltqt vgtlssqlre dhaqlqklqe wqshekdlvs sllaeckanr
      781 tsfarlenai skltelqkvg vekqstmvaq sldamlginl ekfianefki tvvpsivkii
      841 dltrceisgr lettealike stikavkska mfdavaqats tsiqshvett crdilenvlm
      901 psldrlchsl fsqlgeifqk gtreflhqaq ehidkhnqeq vaeamkkvqs dverslqtfl
      961 rdhqaalvpp dfrapfdeav nnalqgletg llqaltgqqe klmnslkeem hgvlkesvaa
     1021 avaegaasrs kmatpvpfad phlllqqitl lmrqgqynma fqqalsvadl atvvstcemv
     1081 sptsifgqhp cplqqpvlls liqqlcadls ssseiklkyl eeavlsldke naitkehmkv
     1141 ilsqlclkln nfimgtashd lgrmarrllm vtqsllns
//
LOCUS       XP_077503418            1176 aa            linear   INV 25-NOV-2025
DEFINITION  enhancer of mRNA-decapping protein 4 homolog Ge-1 isoform X7
            [Amblyomma americanum].
ACCESSION   XP_077503418
VERSION     XP_077503418.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647292.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1176
                     /product="enhancer of mRNA-decapping protein 4 homolog
                     Ge-1 isoform X7"
                     /calculated_mol_wt=128395
     Region          113..441
                     /region_name="Ge1_WD40"
                     /note="WD40 region of Ge1, enhancer of mRNA-decapping
                     protein; pfam16529"
                     /db_xref="CDD:465162"
     Region          303..346
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          351..398
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..1176
                     /gene="Ge-1"
                     /coded_by="XM_077647292.1:96..3626"
                     /db_xref="GeneID:144113906"
ORIGIN      
        1 msshegsida neatrllkdi lhlgkspsap segveanggl deaaggggdp tdgmaaggnl
       61 lesifrssqe nqqeiqqsqq qiclldneed vsvdvcgkev qvvvsrmhgn assnrvkikn
      121 vvdyswelky nygkhiclhm sgaylayalr aanssagvvr vlnlktgqrl lvkgfrgpiq
      181 dmafawlatt vmlavvdvyg tlcvysidnd tsstllfkve rppdevpsey rrvvwcpyvp
      241 ddgsrggnsa eddnskvlva tnleeaevwn vgcivaeygm rtltvpevri glqriiehtk
      301 piidaafapd gtalatagad gqvrffqvym qepdasprcl hqwcphnekp vtclffldnm
      361 kacnpetqfw kyaltgtdhn telklwscet waclqtirfi vspvdrdllp afkveidpta
      421 cyvvlsdvhr kvvyvgqlql dnkaelvsla lfpvtlpvls favveavrcr ykpstdtehv
      481 drldaeseea dsadeertfa ktqegtmvrl ywmttksiqa chivypltas salssavslg
      541 tlsqnstvsg ctdhlsdvsa dadddekagq nkhdadvpsq kgkvhgddds sgfaegkvll
      601 kpsdfvspsn sstafnpcta aastntdplp pttspcvrnt stpvpadeth pgeteaaahe
      661 pslltppase gqevgdagwp rppektegfh vapttttvcd qevgadpleq dqnsahalsr
      721 lellisqhle ennaltqtvg tlssqlredh aqlqklqewq shekdlvssl laeckanrts
      781 farlenaisk ltelqkvgve kqstmvaqsl damlginlek fianefkitv vpsivkiidl
      841 trceisgrle ttealikest ikavkskamf davaqatsts iqshvettcr dilenvlmps
      901 ldrlchslfs qlgeifqkgt reflhqaqeh idkhnqeqva eamkkvqsdv erslqtflrd
      961 hqaalvppdf rapfdeavnn alqgletgll qaltgqqekl mnslkeemhg vlkesvaaav
     1021 aegaasrskm atpvpfadph lllqqitllm rqgqynmafq qalsvadlat vvstcemvsp
     1081 tsifgqhpcp lqqpvllsli qqlcadlsss seiklkylee avlsldkena itkehmkvil
     1141 sqlclklnnf imgtashdlg rmarrllmvt qsllns
//
LOCUS       XP_077503419            1154 aa            linear   INV 25-NOV-2025
DEFINITION  enhancer of mRNA-decapping protein 4 homolog Ge-1 isoform X8
            [Amblyomma americanum].
ACCESSION   XP_077503419
VERSION     XP_077503419.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647293.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1154
                     /product="enhancer of mRNA-decapping protein 4 homolog
                     Ge-1 isoform X8"
                     /calculated_mol_wt=126260
     Region          91..419
                     /region_name="Ge1_WD40"
                     /note="WD40 region of Ge1, enhancer of mRNA-decapping
                     protein; pfam16529"
                     /db_xref="CDD:465162"
     Region          281..324
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          329..376
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..1154
                     /gene="Ge-1"
                     /coded_by="XM_077647293.1:141..3605"
                     /db_xref="GeneID:144113906"
ORIGIN      
        1 mqrspypyse gveangglde aaggggdptd gmaaggnlle sifrssqenq qeiqqsqqqi
       61 clldneedvs vdvcgkevqv vvsrmhgnas snrvkiknvv dyswelkyny gkhiclhmsg
      121 aylayalraa nssagvvrvl nlktgqrllv kgfrgpiqdm afawlattvm lavvdvygtl
      181 cvysidndts stllfkverp pdevpseyrr vvwcpyvpdd gsrggnsaed dnskvlvatn
      241 leeaevwnvg civaeygmrt ltvpevrigl qriiehtkpi idaafapdgt alatagadgq
      301 vrffqvymqe pdasprclhq wcphnekpvt clffldnmka cnpetqfwky altgtdhnte
      361 lklwscetwa clqtirfivs pvdrdllpaf kveidptacy vvlsdvhrkv vyvgqlqldn
      421 kaelvslalf pvtlpvlsfa vveavrcryk pstdtehvdr ldaeseeads adeertfakt
      481 qegtmvrlyw mttksiqach ivypltassa lssavslgtl sqnstvsgct dhlsdvsada
      541 dddekagqnk hdadvpsqkg kvhgdddssg faegkvllkp sdfvspsnss tafnpctaaa
      601 stntdplppt tspcvrntst pvpadethpg eteaaaheps lltppasegq evgdagwprp
      661 pektegfhva pttttvcdqe vgadpleqdq nsahalsrle llisqhleen naltqtvgtl
      721 ssqlredhaq lqklqewqsh ekdlvsslla eckanrtsfa rlenaisklt elqkvgvekq
      781 stmvaqslda mlginlekfi anefkitvvp sivkiidltr ceisgrlett ealikestik
      841 avkskamfda vaqatstsiq shvettcrdi lenvlmpsld rlchslfsql geifqkgtre
      901 flhqaqehid khnqeqvaea mkkvqsdver slqtflrdhq aalvppdfra pfdeavnnal
      961 qgletgllqa ltgqqeklmn slkeemhgvl kesvaaavae gaasrskmat pvpfadphll
     1021 lqqitllmrq gqynmafqqa lsvadlatvv stcemvspts ifgqhpcplq qpvllsliqq
     1081 lcadlsssse iklkyleeav lsldkenait kehmkvilsq lclklnnfim gtashdlgrm
     1141 arrllmvtqs llns
//
LOCUS       XP_077503420             745 aa            linear   INV 25-NOV-2025
DEFINITION  SNF related kinase [Amblyomma americanum].
ACCESSION   XP_077503420
VERSION     XP_077503420.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647294.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..745
                     /product="SNF related kinase"
                     /calculated_mol_wt=80841
     Region          14..271
                     /region_name="STKc_SNRK"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     SNF1-related kinase; cd14074"
                     /db_xref="CDD:270976"
     Site            order(24..28,32,45,47,78,94..97,101,103..104,106,140..141,
                     143,145..146,148,159..160,163,177..181,183,213..216)
                     /site_type="active"
                     /db_xref="CDD:270976"
     Site            order(24..27,32,45,47,78,94..97,101,145..146,148,159..160)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270976"
     Site            order(101,103..104,106,141,143,145,163,177..181,183,
                     213..216)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270976"
     Site            159..181
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270976"
     Region          289..336
                     /region_name="UBA_SNRK"
                     /note="UBA domain of SNF-related serine/threonine-protein
                     kinase (SNRK) and similar proteins mainly found in
                     metazoa; cd14339"
                     /db_xref="CDD:270524"
     CDS             1..745
                     /gene="Snrk"
                     /coded_by="XM_077647294.1:706..2943"
                     /db_xref="GeneID:144113907"
ORIGIN      
        1 mnrpkhqqqy dpkiaglydl gdtlgrghfa vvklarhvft geqvavkvid ktklddvsra
       61 hlfqevrcmk lvqhpnvvrl yevidtqtkl ylvleygdgg dmydyimkhd rgvseqaark
      121 yfrqivhaiw ychklhvvhr dlkpenvvff eklemvkltd fgfsnkfcpg qkletscgsl
      181 aysapeillg dsydapkvdv wslgvilyml vcghapfqea ndsetltmim dckytippyv
      241 sedckrmiar mlirdpdkra sleeiaadpw ltagdapqpa dhlplihreh lseedhshii
      301 qkmvngcias kddilealdr neynymtaty yllaerklra qrqekaqqln rtsradlspv
      361 avtpgvssaa pspaqerpnv aqvlaspggv psialdssag hplasllgvc sgpleddssl
      421 avprsnlplg rkcsivreed dltslapshs ppirsavfce egeppepder avrpvlslpa
      481 apsassppts dgapqhqspt vrlrcipgrr lhavksspql lggagdmrvr pgvgklrvha
      541 tgrnrttscs ssddeeadkr rhpptakgdt plqgspsgsv lpsphahdeg ysadstdsld
      601 mlpdpaprlt nsvscgdltv eesgvpknde gasssavqca dvvlveemap adaprisnsf
      661 slaaglsclg rkssreemrs srsrlgssla klvpngsrns asnnagagpl kpqldinqng
      721 frkgrsksqs gvvdvklask cctlc
//
LOCUS       XP_077503421             655 aa            linear   INV 25-NOV-2025
DEFINITION  nuclear receptor coactivator protein neosin [Amblyomma americanum].
ACCESSION   XP_077503421
VERSION     XP_077503421.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647295.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (GKSP01013762.1) annotated
            using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 9 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..655
                     /product="nuclear receptor coactivator protein neosin"
                     /calculated_mol_wt=73029
     Region          17..78
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          96..>275
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     CDS             1..655
                     /gene="Neos"
                     /coded_by="XM_077647295.1:194..2161"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 9 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144113908"
ORIGIN      
        1 mdthmssdts dpakassriy vgnlkkhier edlqklfsky geiqgislhk ggfgfvqfke
       61 edeaqaavkg eggiffkgcl lqlrvcssqd vrrsyrgssr erdrefsdhd rrheanmprd
      121 rspvhrdyde refkrddrpp pskaydrspp rrgddyssdr rfgrdegryg rdappyggrg
      181 ddylrggqgg pspreshsry sredryggrd ergygrddpp rpyprpegng rygrdgywgg
      241 rddyshlgrd eppmgygrde phppprvrme avpvpppfrp eanlrkpndc eiivlhrqnr
      301 nyaemverrl knlgmqvdll flkdeallsr aledltqrgt lfavvvtdqh evhgsvtvni
      361 lygvpqehrn mplddslklv arhfreqvea qqaaaggpdr lgpdrelqfl lkllvdskyl
      421 sipeldriik ylterrdkql mlergesahq qprtvsstap sanveperpk appveeekpt
      481 qqelqqrils migqpgpisp ttvvkqaplp tpvqpqqqap aaapasdvpt styinfdnps
      541 vqkaldnlih ngsnllktls sanssgaspa lssqqpakpp sgygygdgea yggsapginn
      601 sfgghagqqy qqppplmrgp ggpptpgamr hplmgtqvqr pmmpasrhgg gmpry
//
LOCUS       XP_077503422             336 aa            linear   INV 25-NOV-2025
DEFINITION  regulator of microtubule dynamics protein 1-like [Amblyomma
            americanum].
ACCESSION   XP_077503422
VERSION     XP_077503422.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647296.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..336
                     /product="regulator of microtubule dynamics protein
                     1-like"
                     /calculated_mol_wt=38009
     Region          213..>314
                     /region_name="Spy"
                     /note="Predicted O-linked N-acetylglucosamine transferase,
                     SPINDLY family [Posttranslational modification, protein
                     turnover, chaperones]; COG3914"
                     /db_xref="CDD:443119"
     Region          226..276
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          282..309
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     CDS             1..336
                     /gene="LOC144113910"
                     /coded_by="XM_077647296.1:79..1089"
                     /db_xref="GeneID:144113910"
ORIGIN      
        1 mcyrarlwsl rygfapfaig lrvrsaglss kfqgwrhlta agqlavsgvv pqvfllcgla
       61 lldmsevera kqeadrlhea gdheasykml lhhkecgdpd llwrlarvtf krcelapkae
      121 keaglaegia yldkavqcgg edlgpvhkwy siligskkdh pttkerihdg flikkhvqas
      181 qqyiflsdtp fraiscgkdy fsifaflyse vlllflqrai elmpqdplny yvlgnwcyev
      241 agtpwlirka gqlvfseips styeealkhf lkaeeikpnf ytrnllmiak sllelnrkde
      301 avtylkrcvn lsanptvddk rvceeaqqsl kklgms
//
LOCUS       XP_077503423             125 aa            linear   INV 25-NOV-2025
DEFINITION  RAB interacting factor STRAT [Amblyomma americanum].
ACCESSION   XP_077503423
VERSION     XP_077503423.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647297.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..125
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..125
                     /product="RAB interacting factor STRAT"
                     /calculated_mol_wt=13912
     Site            order(33,36,97,100)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:238150"
     Region          37..124
                     /region_name="Mss4"
                     /note="Mss4 protein; pfam04421"
                     /db_xref="CDD:461301"
     Site            order(76..78,82,99)
                     /site_type="other"
                     /note="putative Rab GTPase interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:238150"
     CDS             1..125
                     /gene="strat"
                     /coded_by="XM_077647297.1:247..624"
                     /db_xref="GeneID:144113911"
ORIGIN      
        1 mddkenaprg vaggekekdk lvengknkvp ieckycssri lnvgmcelvc tqydlptvdg
       61 dskmlekvtd fwcvndiytf enigfsntvg qtkylacadc ergpvgwhsl etrksfvals
      121 rvrhl
//
LOCUS       XP_077503424            1152 aa            linear   INV 25-NOV-2025
DEFINITION  RAB11-binding protein RELCH homolog isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503424
VERSION     XP_077503424.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647298.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1152
                     /product="RAB11-binding protein RELCH homolog isoform X1"
                     /calculated_mol_wt=128556
     Site            order(919..920,923,926..927,958..959,962,965..966,969,
                     1001..1002,1005,1008..1009,1012,1044..1045,1052,
                     1055..1056)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          941..969
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          979..1013
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          1022..1056
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..1152
                     /gene="LOC144113912"
                     /coded_by="XM_077647298.1:65..3523"
                     /db_xref="GeneID:144113912"
ORIGIN      
        1 mrrqlysmaa glksgameed iskcssaset pivlpapfel esvydvtidk iaskllkdnf
       61 iltalefhae llesgrelpr lrdyfsnpgn fekhshstfd aspvlprtss vqtfdsldfa
      121 rysddgekql dervavlefe lrkaretikn lranltvvte sdpntpdngs ehttvledpl
      181 rpherravnf llneyllkhd ykltsitfsd enelqdfedw ddvglnipkp pdlvqlyrsy
      241 nrhsrlccdv aecgvqtefe dsdkqedlls nlqalaaekt ileaqvnclt mqlekanqen
      301 lvltaeldkv lfsraelpfk pdlplemvad tlsdavepct ptpavargsp rkkvpeekgd
      361 sseehftlrh smctadplpn ervhntapce desatksvgl eapksrrktp qafrealmnv
      421 chhhcelsds rlakdvttva ssgkevvlml arclphivpn vllakreelv plllatinlh
      481 pdaterdall nllfnltkrp ddaqrriila glvsvaqclg herveaellp qcweqinhky
      541 lerrllvaes cgylasqvas eirsslllsm lqqmlkeeke dmvrecaars lavllvyvdd
      601 qdkfsqavdl allvvadara smaevaqtvl lpsvaawale igrledtlls tlmklledhi
      661 ralalrsadg stalsqseeq hsvhlirtlg allpflfvhv vetgpyvnrl nfterssscv
      721 lgddrvprpq splldplkis nygldmpali aafdehigqe wyktwpafdw llskflpflt
      781 giairmepss raavhtlsel llsfcrlfgh tfthckvkpa fsalvpltda fsqeakdtlt
      841 savgpvyasg ilaafscesd ckelmaflrn llfvvslcel plssmesvil elsmdknyhe
      901 vllsvlwegv vhntalvrnn agrlfellvg tvadpllksr vtpalvtlas dpeisvrigt
      961 vrpfgailat ssqkdlldka fmqlqsfled pqhrddhsmq aefihmfasl gpnteprfrd
     1021 efilphlanl amrnnqnpnd ssraqimell leaystlsct yisehaisda flpglrslle
     1081 darqfapche pavaamiadf eakldctrer apslsspmas meemkrkmtk iftvpptnar
     1141 snlpnifqlr kk
//
LOCUS       XP_077503426            1054 aa            linear   INV 25-NOV-2025
DEFINITION  RAB11-binding protein RELCH homolog isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503426
VERSION     XP_077503426.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647300.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1054
                     /product="RAB11-binding protein RELCH homolog isoform X2"
                     /calculated_mol_wt=118033
     Site            order(821..822,825,828..829,860..861,864,867..868,871,
                     903..904,907,910..911,914,946..947,954,957..958)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          843..871
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          881..915
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          924..958
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..1054
                     /gene="LOC144113912"
                     /coded_by="XM_077647300.1:303..3467"
                     /db_xref="GeneID:144113912"
ORIGIN      
        1 mllqccrerq vsrhlipwtl rgirmmerns wmkellvvle felrkareti knlranltvv
       61 tesdpntpdn gsehttvled plrpherrav nfllneyllk hdykltsitf sdenelqdfe
      121 dwddvglnip kppdlvqlyr synrhsrlcc dvaecgvqte fedsdkqedl lsnlqalaae
      181 ktileaqvnc ltmqlekanq enlvltaeld kvlfsraelp fkpdlplemv adtlsdavep
      241 ctptpavarg sprkkvpeek gdsseehftl rhsmctadpl pnervhntap cedesatksv
      301 gleapksrrk tpqafrealm nvchhhcels dsrlakdvtt vassgkevvl mlarclphiv
      361 pnvllakree lvplllatin lhpdaterda llnllfnltk rpddaqrrii laglvsvaqc
      421 lgherveael lpqcweqinh kylerrllva escgylasqv aseirsslll smlqqmlkee
      481 kedmvrecaa rslavllvyv ddqdkfsqav dlallvvada rasmaevaqt vllpsvaawa
      541 leigrledtl lstlmklled hiralalrsa dgstalsqse eqhsvhlirt lgallpflfv
      601 hvvetgpyvn rlnftersss cvlgddrvpr pqsplldplk isnygldmpa liaafdehig
      661 qewyktwpaf dwllskflpf ltgiairmep ssraavhtls elllsfcrlf ghtfthckvk
      721 pafsalvplt dafsqeakdt ltsavgpvya sgilaafsce sdckelmafl rnllfvvslc
      781 elplssmesv ilelsmdkny hevllsvlwe gvvhntalvr nnagrlfell vgtvadpllk
      841 srvtpalvtl asdpeisvri gtvrpfgail atssqkdlld kafmqlqsfl edpqhrddhs
      901 mqaefihmfa slgpnteprf rdefilphla nlamrnnqnp ndssraqime llleaystls
      961 ctyisehais daflpglrsl ledarqfapc hepavaamia dfeakldctr erapslsspm
     1021 asmeemkrkm tkiftvpptn arsnlpnifq lrkk
//
LOCUS       XP_077503427             752 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113913 [Amblyomma americanum].
ACCESSION   XP_077503427
VERSION     XP_077503427.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647301.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..752
                     /product="uncharacterized protein LOC144113913"
                     /calculated_mol_wt=82737
     Region          432..522
                     /region_name="PWWP_MUM1-like"
                     /note="PWWP domain found in mutated melanoma-associated
                     antigen 1 (MUM-1) and similar proteins; cd06080"
                     /db_xref="CDD:438967"
     Site            order(445,448,466)
                     /site_type="other"
                     /note="putative methyl-histone binding site [polypeptide
                     binding]"
                     /db_xref="CDD:438967"
     Site            446..449
                     /site_type="other"
                     /note="PWWP sequence motif"
                     /db_xref="CDD:438967"
     CDS             1..752
                     /gene="LOC144113913"
                     /coded_by="XM_077647301.1:226..2484"
                     /db_xref="GeneID:144113913"
ORIGIN      
        1 meepefspst pvvaaaeeqh vsensipdna asaattsesv crgelsahkl seplpasemv
       61 avgssaesal pamdsgldkp elvggipvvc kgkvletvve gkqallqlak epvkqeeals
      121 qcpkepqkea mpdavhdvme qatsvsgade pamttdsvlg tdtetgrrts crvrtpsvli
      181 gapggdravp mseptaqsap rkrkltfeqi ppnlqavvsr nlelptltks lpsrmermkf
      241 gnrsevavrr kaprksnvkt cvthfrqqgl srsasdnths lyacqkcsfr tsrmdnlvrh
      301 kkadcafikd ffswdantft eatemskkrs lspdvlvese steeasktpv akckgsarks
      361 eastrtsekk ksverdeksk qalgvceess edeaptskrl ppiplesvyl ssedeaptst
      421 rsppiplqkl efgvddvvwv ewkkhlhwpg ivtkimprkk karirlvdal tnescvlasl
      481 nkivnfnnae rnrlylsrar vgqtsgalvk avqrveeylr krclgsnvgp rqffwdevpf
      541 tegppsvnsd dfyldalcsd sglasegagd avdevaarik ekrkqcnerl lqciingnvn
      601 shllgvlngt vdserhhlfy sslsrertql krlswfgpid dvqqqeeiye fcfellkans
      661 dldasfdias yvievwcpea vmkalsltlg idmekaeevl lratvlskae hearrhehlt
      721 pagtdateeh darvsnelrd igiadimatf ds
//
LOCUS       XP_077503428             617 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113914 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503428
VERSION     XP_077503428.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647302.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..617
                     /product="uncharacterized protein LOC144113914 isoform X1"
                     /calculated_mol_wt=70040
     Site            order(1,3,7..8,11..13,15..16,20,23,33,35,37,41,48,60..62,
                     64..65,69,72,81,83,85,89..90,93..95,97..98,102,105)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1..33
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <6..>105
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          35..81
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <161..347
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          168..250
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          <417..>551
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(458,460,464..465,468..470,472..473,477,480,493,495,
                     497,501..502,507..509,510..511,515,518,527,529,531,
                     535..536,539..541,543..544,547,550)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          458..493
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          495..527
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..617
                     /gene="LOC144113914"
                     /coded_by="XM_077647302.1:230..2083"
                     /db_xref="GeneID:144113914"
ORIGIN      
        1 meeselfvav ttgdessvrr lldnnvvpft vtndngenfl hilakqnlav lspeettlrr
       61 qrrsniiyml cddglnpshr ngvgqtpmhy aiqnsdpyll ktllqcspnp pfeicdaivp
      121 splqevlnkf kpglcdairr rciadvrriv nmwcrtdgfv dgyslkelal esgheeiisl
      181 ilgvepsmrl vhhvlarnve kvrkilcter vhlnldlrnm sdqgapilyy aiankdvdiv
      241 rllvlsgchv ftlmrdadnq eipvlfsals aemspelada lmprnllgqq evirrllyks
      301 rtilevaldk rvpprtfell lhrggaellv grnqdnktvr diaklanrfh yveaidkvvk
      361 dwvkdstsyl dqwqilalcg ydhittlfar vrtiketfke lppdtveyle gvtlyqeqia
      421 nlaravedgd yerfeelrty kstvfrtnmc wqacqkgdgq pllhravlfn rpsivktilq
      481 nipygqsidt lldqhhrtal hyaygmrnge hlvqllmnag csehtldrig kepldfkvhc
      541 gspametllr hirnkvpfee srvwnsaghq kikskccqga secnerpvlp hkvhaltcgc
      601 qatnvpdvnk tiwcail
//
LOCUS       XP_077503429             612 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113914 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503429
VERSION     XP_077503429.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647303.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..612
                     /product="uncharacterized protein LOC144113914 isoform X2"
                     /calculated_mol_wt=69442
     Site            order(1,3,7..8,11..13,15..16,20,23,33,35,37,41,48,60..62,
                     64..65,69,72,81,83,85,89..90,93..95,97..98,102,105)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1..33
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <6..>105
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          35..81
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <161..347
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          168..250
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          <412..>546
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(453,455,459..460,463..465,467..468,472,475,488,490,
                     492,496..497,502..504,505..506,510,513,522,524,526,
                     530..531,534..536,538..539,542,545)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          453..488
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          490..522
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..612
                     /gene="LOC144113914"
                     /coded_by="XM_077647303.1:229..2067"
                     /db_xref="GeneID:144113914"
ORIGIN      
        1 meeselfvav ttgdessvrr lldnnvvpft vtndngenfl hilakqnlav lspeettlrr
       61 qrrsniiyml cddglnpshr ngvgqtpmhy aiqnsdpyll ktllqcspnp pfeicdaivp
      121 splqevlnkf kpglcdairr rciadvrriv nmwcrtdgfv dgyslkelal esgheeiisl
      181 ilgvepsmrl vhhvlarnve kvrkilcter vhlnldlrnm sdqgapilyy aiankdvdiv
      241 rllvlsgchv ftlmrdadnq eipvlfsals aemspelada lmprnllgqq evirrllyks
      301 rtilevaldk rvpprtfell lhrggaellv grnqdnktvr diaklanrfh yveaidkvvk
      361 dwvkdstsyl dqwqilalcg ydhittlfar etfkelppdt veylegvtly qeqianlara
      421 vedgdyerfe elrtykstvf rtnmcwqacq kgdgqpllhr avlfnrpsiv ktilqnipyg
      481 qsidtlldqh hrtalhyayg mrngehlvql lmnagcseht ldrigkepld fkvhcgspam
      541 etllrhirnk vpfeesrvwn saghqkiksk ccqgasecne rpvlphkvha ltcgcqatnv
      601 pdvnktiwca il
//
LOCUS       XP_077503430             556 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113914 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503430
VERSION     XP_077503430.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647304.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..556
                     /product="uncharacterized protein LOC144113914 isoform X3"
                     /calculated_mol_wt=62875
     Site            order(1,3,7..8,11..13,15..16,20,23,33,35,37,41,48,60..62,
                     64..65,69,72,81,83,85,89..90,93..95,97..98,102,105)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1..33
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <6..>105
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          35..81
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          168..250
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          <356..>490
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(397,399,403..404,407..409,411..412,416,419,432,434,
                     436,440..441,446..448,449..450,454,457,466,468,470,
                     474..475,478..480,482..483,486,489)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          397..432
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          434..466
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..556
                     /gene="LOC144113914"
                     /coded_by="XM_077647304.1:231..1901"
                     /db_xref="GeneID:144113914"
ORIGIN      
        1 meeselfvav ttgdessvrr lldnnvvpft vtndngenfl hilakqnlav lspeettlrr
       61 qrrsniiyml cddglnpshr ngvgqtpmhy aiqnsdpyll ktllqcspnp pfeicdaivp
      121 splqevlnkf kpglcdairr rciadvrriv nmwcrtdgfv dgyslkelal esgheeiisl
      181 ilgvepsmrl vhhvlarnve kvrkilcter vhlnldlrnm sdqgapilyy aiankdvdiv
      241 rllvlsgchv ftlmrdadnq eipvlfsals aemspelada lmprnllgqq evirrllyks
      301 rtilevaldk rvpprtfell lhrggaellv grnqetfkel ppdtveyleg vtlyqeqian
      361 laravedgdy erfeelrtyk stvfrtnmcw qacqkgdgqp llhravlfnr psivktilqn
      421 ipygqsidtl ldqhhrtalh yaygmrngeh lvqllmnagc sehtldrigk epldfkvhcg
      481 spametllrh irnkvpfees rvwnsaghqk ikskccqgas ecnerpvlph kvhaltcgcq
      541 atnvpdvnkt iwcail
//
LOCUS       XP_077503431             549 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113914 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503431
VERSION     XP_077503431.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647305.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..549
                     /product="uncharacterized protein LOC144113914 isoform X4"
                     /calculated_mol_wt=62295
     Region          <93..279
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          100..182
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          <349..>483
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(390,392,396..397,400..402,404..405,409,412,425,427,
                     429,433..434,439..441,442..443,447,450,459,461,463,
                     467..468,471..473,475..476,479,482)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          390..425
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          427..459
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..549
                     /gene="LOC144113914"
                     /coded_by="XM_077647305.1:589..2238"
                     /db_xref="GeneID:144113914"
ORIGIN      
        1 mlcddglnps hrngvgqtpm hyaiqnsdpy llktllqcsp nppfeicdai vpsplqevln
       61 kfkpglcdai rrrciadvrr ivnmwcrtdg fvdgyslkel alesgheeii slilgvepsm
      121 rlvhhvlarn vekvrkilct ervhlnldlr nmsdqgapil yyaiankdvd ivrllvlsgc
      181 hvftlmrdad nqeipvlfsa lsaemspela dalmprnllg qqevirrlly ksrtileval
      241 dkrvpprtfe lllhrggael lvgrnqdnkt vrdiaklanr fhyveaidkv vkdwvkdsts
      301 yldqwqilal cgydhittlf arvrtiketf kelppdtvey legvtlyqeq ianlaraved
      361 gdyerfeelr tykstvfrtn mcwqacqkgd gqpllhravl fnrpsivkti lqnipygqsi
      421 dtlldqhhrt alhyaygmrn gehlvqllmn agcsehtldr igkepldfkv hcgspametl
      481 lrhirnkvpf eesrvwnsag hqkikskccq gasecnerpv lphkvhaltc gcqatnvpdv
      541 nktiwcail
//
LOCUS       XP_077503432             530 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113914 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077503432
VERSION     XP_077503432.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647306.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..530
                     /product="uncharacterized protein LOC144113914 isoform X5"
                     /calculated_mol_wt=60302
     Region          <74..260
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          81..163
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          <330..>464
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(371,373,377..378,381..383,385..386,390,393,406,408,
                     410,414..415,420..422,423..424,428,431,440,442,444,
                     448..449,452..454,456..457,460,463)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          371..406
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          408..440
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..530
                     /gene="LOC144113914"
                     /coded_by="XM_077647306.1:308..1900"
                     /db_xref="GeneID:144113914"
ORIGIN      
        1 mhyaiqnsdp yllktllqcs pnppfeicda ivpsplqevl nkfkpglcda irrrciadvr
       61 rivnmwcrtd gfvdgyslke lalesgheei islilgveps mrlvhhvlar nvekvrkilc
      121 tervhlnldl rnmsdqgapi lyyaiankdv divrllvlsg chvftlmrda dnqeipvlfs
      181 alsaemspel adalmprnll gqqevirrll yksrtileva ldkrvpprtf elllhrggae
      241 llvgrnqdnk tvrdiaklan rfhyveaidk vvkdwvkdst syldqwqila lcgydhittl
      301 farvrtiket fkelppdtve ylegvtlyqe qianlarave dgdyerfeel rtykstvfrt
      361 nmcwqacqkg dgqpllhrav lfnrpsivkt ilqnipygqs idtlldqhhr talhyaygmr
      421 ngehlvqllm nagcsehtld rigkepldfk vhcgspamet llrhirnkvp feesrvwnsa
      481 ghqkikskcc qgasecnerp vlphkvhalt cgcqatnvpd vnktiwcail
//
LOCUS       XP_077503433             420 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113914 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077503433
VERSION     XP_077503433.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647307.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..420
                     /product="uncharacterized protein LOC144113914 isoform X6"
                     /calculated_mol_wt=47680
     Site            order(1,3,7..8,11..13,15..16,20,23,33,35,37,41,48,60..62,
                     64..65,69,72,81,83,85,89..90,93..95,97..98,102,105)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1..33
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <6..>105
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          35..81
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          168..250
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     CDS             1..420
                     /gene="LOC144113914"
                     /coded_by="XM_077647307.1:232..1494"
                     /db_xref="GeneID:144113914"
ORIGIN      
        1 meeselfvav ttgdessvrr lldnnvvpft vtndngenfl hilakqnlav lspeettlrr
       61 qrrsniiyml cddglnpshr ngvgqtpmhy aiqnsdpyll ktllqcspnp pfeicdaivp
      121 splqevlnkf kpglcdairr rciadvrriv nmwcrtdgfv dgyslkelal esgheeiisl
      181 ilgvepsmrl vhhvlarnve kvrkilcter vhlnldlrnm sdqgapilyy aiankdvdiv
      241 rllvlsgchv ftlmrdadnq eipvlfsals aemspelada lmprnllgqq evirrllyks
      301 rtilevaldk rvpprtfell lhrggaellv grnqdnktvr diaklanrfh yveaidkvvk
      361 dwvkdstsyl dqwqilalcg ydhittlfar vrtiketfke lppdtveyle gvtlyqgrar
//
LOCUS       XP_077503434             415 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113914 isoform X7 [Amblyomma
            americanum].
ACCESSION   XP_077503434
VERSION     XP_077503434.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647308.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..415
                     /product="uncharacterized protein LOC144113914 isoform X7"
                     /calculated_mol_wt=47082
     Site            order(1,3,7..8,11..13,15..16,20,23,33,35,37,41,48,60..62,
                     64..65,69,72,81,83,85,89..90,93..95,97..98,102,105)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1..33
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <6..>105
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          35..81
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          168..250
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     CDS             1..415
                     /gene="LOC144113914"
                     /coded_by="XM_077647308.1:232..1479"
                     /db_xref="GeneID:144113914"
ORIGIN      
        1 meeselfvav ttgdessvrr lldnnvvpft vtndngenfl hilakqnlav lspeettlrr
       61 qrrsniiyml cddglnpshr ngvgqtpmhy aiqnsdpyll ktllqcspnp pfeicdaivp
      121 splqevlnkf kpglcdairr rciadvrriv nmwcrtdgfv dgyslkelal esgheeiisl
      181 ilgvepsmrl vhhvlarnve kvrkilcter vhlnldlrnm sdqgapilyy aiankdvdiv
      241 rllvlsgchv ftlmrdadnq eipvlfsals aemspelada lmprnllgqq evirrllyks
      301 rtilevaldk rvpprtfell lhrggaellv grnqdnktvr diaklanrfh yveaidkvvk
      361 dwvkdstsyl dqwqilalcg ydhittlfar etfkelppdt veylegvtly qgrar
//
LOCUS       XP_077503435             359 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113914 isoform X8 [Amblyomma
            americanum].
ACCESSION   XP_077503435
VERSION     XP_077503435.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647309.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..359
                     /product="uncharacterized protein LOC144113914 isoform X8"
                     /calculated_mol_wt=40515
     Site            order(1,3,7..8,11..13,15..16,20,23,33,35,37,41,48,60..62,
                     64..65,69,72,81,83,85,89..90,93..95,97..98,102,105)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1..33
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <6..>105
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          35..81
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          168..250
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     CDS             1..359
                     /gene="LOC144113914"
                     /coded_by="XM_077647309.1:233..1312"
                     /db_xref="GeneID:144113914"
ORIGIN      
        1 meeselfvav ttgdessvrr lldnnvvpft vtndngenfl hilakqnlav lspeettlrr
       61 qrrsniiyml cddglnpshr ngvgqtpmhy aiqnsdpyll ktllqcspnp pfeicdaivp
      121 splqevlnkf kpglcdairr rciadvrriv nmwcrtdgfv dgyslkelal esgheeiisl
      181 ilgvepsmrl vhhvlarnve kvrkilcter vhlnldlrnm sdqgapilyy aiankdvdiv
      241 rllvlsgchv ftlmrdadnq eipvlfsals aemspelada lmprnllgqq evirrllyks
      301 rtilevaldk rvpprtfell lhrggaellv grnqetfkel ppdtveyleg vtlyqgrar
//
LOCUS       XP_077503436             259 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: vesicle-associated membrane protein-associated
            protein B-like [Amblyomma americanum].
ACCESSION   XP_077503436
VERSION     XP_077503436.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647310.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..259
                     /product="LOW QUALITY PROTEIN: vesicle-associated membrane
                     protein-associated protein B-like"
                     /calculated_mol_wt=28698
     Region          6..96
                     /region_name="Motile_Sperm"
                     /note="MSP (Major sperm protein) domain; pfam00635"
                     /db_xref="CDD:459882"
     Region          161..>192
                     /region_name="ZapB"
                     /note="Cell division protein ZapB; pfam06005"
                     /db_xref="CDD:428718"
     CDS             1..259
                     /gene="LOC144113918"
                     /coded_by="XM_077647310.1:178..957"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:144113918"
ORIGIN      
        1 mskqvlqlep dselhfkgpf ndvvtshlkl snptdkricf kvkttapkry cvrpnsgile
       61 pkqavqvavm lqpfeydpte knkhkfmvqt miapdgdvnq dtvwrdanpe slmdsklrcv
      121 fempteaapq nnldvstshg dekgaapkge capkspkasn leadlkkate ecrrlreeva
      181 slrqenaalk edglrqrmlq taatsetvts haapettsqm thrtkemsma hpsgvsplml
      241 tlfafvmvvt gmlvgkwlf
//
LOCUS       XP_077503438             344 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113920 [Amblyomma americanum].
ACCESSION   XP_077503438
VERSION     XP_077503438.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647312.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..344
                     /product="uncharacterized protein LOC144113920"
                     /calculated_mol_wt=37777
     Region          14..81
                     /region_name="EF-hand_7"
                     /note="EF-hand domain pair; pfam13499"
                     /db_xref="CDD:463900"
     Region          93..313
                     /region_name="cysteine_hydrolases"
                     /note="Cysteine hydrolases; This family contains
                     amidohydrolases, like CSHase (N-carbamoylsarcosine
                     amidohydrolase), involved in creatine metabolism and
                     nicotinamidase, converting nicotinamide to nicotinic acid
                     and ammonia in the pyridine nucleotide cycle. It...;
                     cl00220"
                     /db_xref="CDD:444760"
     Site            order(98,223,268)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238245"
     Site            263..264
                     /site_type="active"
                     /note="conserved cis-peptide bond [active]"
                     /db_xref="CDD:238245"
     CDS             1..344
                     /gene="LOC144113920"
                     /coded_by="XM_077647312.1:176..1210"
                     /db_xref="GeneID:144113920"
ORIGIN      
        1 msklelcsqs ksprelftql vlqqdamlnk edfssflssl ftdfdevpta ekqtaikflf
       61 etfdsnhdgg idisefetmw tqwaqailcp kwalivvdvq ndfitgtlsv knlaekqdpe
      121 givpvindla enlpwelvvy sqdwhppdhi sffenkdhrh fhasskvstg hakvfdtvvf
      181 vddksssgsi eqtlwpahci qgtwgaelhk dlrvpssalk irkgispdvd sysafwdndk
      241 rsstvldnel karkitgvvi cgiaydvcva stashamclg yqtvivdnas cgtcaaatea
      301 tksclaakhc vflessevkq lisggarplq lglqlakats akni
//
LOCUS       XP_077503439             344 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113920 [Amblyomma americanum].
ACCESSION   XP_077503439
VERSION     XP_077503439.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647313.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..344
                     /product="uncharacterized protein LOC144113920"
                     /calculated_mol_wt=37777
     Region          14..81
                     /region_name="EF-hand_7"
                     /note="EF-hand domain pair; pfam13499"
                     /db_xref="CDD:463900"
     Region          93..313
                     /region_name="cysteine_hydrolases"
                     /note="Cysteine hydrolases; This family contains
                     amidohydrolases, like CSHase (N-carbamoylsarcosine
                     amidohydrolase), involved in creatine metabolism and
                     nicotinamidase, converting nicotinamide to nicotinic acid
                     and ammonia in the pyridine nucleotide cycle. It...;
                     cl00220"
                     /db_xref="CDD:444760"
     Site            order(98,223,268)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238245"
     Site            263..264
                     /site_type="active"
                     /note="conserved cis-peptide bond [active]"
                     /db_xref="CDD:238245"
     CDS             1..344
                     /gene="LOC144113920"
                     /coded_by="XM_077647313.1:174..1208"
                     /db_xref="GeneID:144113920"
ORIGIN      
        1 msklelcsqs ksprelftql vlqqdamlnk edfssflssl ftdfdevpta ekqtaikflf
       61 etfdsnhdgg idisefetmw tqwaqailcp kwalivvdvq ndfitgtlsv knlaekqdpe
      121 givpvindla enlpwelvvy sqdwhppdhi sffenkdhrh fhasskvstg hakvfdtvvf
      181 vddksssgsi eqtlwpahci qgtwgaelhk dlrvpssalk irkgispdvd sysafwdndk
      241 rsstvldnel karkitgvvi cgiaydvcva stashamclg yqtvivdnas cgtcaaatea
      301 tksclaakhc vflessevkq lisggarplq lglqlakats akni
//
LOCUS       XP_077503440             244 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113921 [Amblyomma americanum].
ACCESSION   XP_077503440
VERSION     XP_077503440.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647314.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..244
                     /product="uncharacterized protein LOC144113921"
                     /calculated_mol_wt=27562
     Region          <8..>117
                     /region_name="GKAP"
                     /note="Guanylate-kinase-associated protein (GKAP) protein;
                     pfam03359"
                     /db_xref="CDD:460897"
     CDS             1..244
                     /gene="LOC144113921"
                     /coded_by="XM_077647314.1:140..874"
                     /db_xref="GeneID:144113921"
ORIGIN      
        1 mapttsnfrd lveeerrrla smcqywesvs lepniseeaq gyirtaigqa nllmkerfaq
       61 faglihicen nlgekkttce dlqgfwdmiy fqvedvnkkf gqlvklqena wcteqpeats
      121 tdltragrea ndmkntrhis kkcapselqk ariaasrrri aeakarmact aseqtptash
      181 ekenavifea psfflvsspa rpkqthtrmp revpegvrst pkqkdflpli cvtrsakkvl
      241 ishn
//
LOCUS       XP_077503441             399 aa            linear   INV 25-NOV-2025
DEFINITION  mevalonate diphosphate decarboxylase isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503441
VERSION     XP_077503441.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647315.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..399
                     /product="mevalonate diphosphate decarboxylase isoform X1"
                     /calculated_mol_wt=44176
     Region          11..333
                     /region_name="MDD_C"
                     /note="Mevalonate 5-diphosphate decarboxylase C-terminal
                     domain; cl28577"
                     /db_xref="CDD:475042"
     CDS             1..399
                     /gene="Mvd"
                     /coded_by="XM_077647315.1:363..1562"
                     /db_xref="GeneID:144113922"
ORIGIN      
        1 mdhitkindg lymtttkapv niavvkywgk tnedliipan dslsltlste hlcatttvav
       61 gkkfredriw lngkeesmrs arlqnclhei rkrsrealhm hndlpdytgw nvhicsmnnf
      121 ptsaglassa agyaclvqsl gtlfhiegdl sgiarrgsgs acrsmyggfv awlrgtnpdg
      181 sdsvakqias sdhwpqmrvi ilvasdakkd tssshgmmrt metstlalhr venivpqhmk
      241 dmtgailnrd fnkfaeitmk esnqlhavcl dsfppirymn saswdvvtlv hrynrfydtn
      301 klaysfdagp naclfllees lpevlalvey afpttarevd ffrgapavig klsdelknfm
      361 nvspqqdaik faiithvgdg pkphdgleah lldvhgypk
//
LOCUS       XP_077503442             322 aa            linear   INV 25-NOV-2025
DEFINITION  mevalonate diphosphate decarboxylase isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503442
VERSION     XP_077503442.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647316.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..322
                     /product="mevalonate diphosphate decarboxylase isoform X2"
                     /calculated_mol_wt=35585
     Region          <3..256
                     /region_name="MDD_C"
                     /note="Mevalonate 5-diphosphate decarboxylase C-terminal
                     domain; cl28577"
                     /db_xref="CDD:475042"
     CDS             1..322
                     /gene="Mvd"
                     /coded_by="XM_077647316.1:218..1186"
                     /db_xref="GeneID:144113922"
ORIGIN      
        1 mrsarlqncl heirkrsrea lhmhndlpdy tgwnvhicsm nnfptsagla ssaagyaclv
       61 qslgtlfhie gdlsgiarrg sgsacrsmyg gfvawlrgtn pdgsdsvakq iassdhwpqm
      121 rviilvasda kkdtssshgm mrtmetstla lhrvenivpq hmkdmtgail nrdfnkfaei
      181 tmkesnqlha vcldsfppir ymnsaswdvv tlvhrynrfy dtnklaysfd agpnaclfll
      241 eeslpevlal veyafpttar evdffrgapa vigklsdelk nfmnvspqqd aikfaiithv
      301 gdgpkphdgl eahlldvhgy pk
//
LOCUS       XP_077503443             713 aa            linear   INV 25-NOV-2025
DEFINITION  protein arginine N-methyltransferase 7-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503443
VERSION     XP_077503443.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647317.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..713
                     /product="protein arginine N-methyltransferase 7-like
                     isoform X1"
                     /calculated_mol_wt=79358
     Region          38..>224
                     /region_name="COG4076"
                     /note="Predicted RNA methylase [General function
                     prediction only]"
                     /db_xref="CDD:443253"
     Region          405..>551
                     /region_name="COG4076"
                     /note="Predicted RNA methylase [General function
                     prediction only]"
                     /db_xref="CDD:443253"
     CDS             1..713
                     /gene="LOC144113923"
                     /coded_by="XM_077647317.1:155..2296"
                     /db_xref="GeneID:144113923"
ORIGIN      
        1 madsyyvenp mtgkdewktq aveydhhqdv arsafgdmlh dgernrkyhi alceaidavr
       61 argekvvvld lgtgsgilam maakrgadvv yaceafepva qcaeqvirdn hlgdrihvir
      121 kkstsiqvgp vvdsgalwrl levcscsrss rkvtasasel gkdmeeranv lvtevfdtel
      181 igegaietfv halkellepa cvvvphaatm yaqvvhspfl rshhtlssvc mtpelqikvp
      241 qsvqtcagts avhdiqlsql qesdflpite plpifrydft daktmpyedf tvsvtkatnr
      301 gtghavlmww aldmncsgsi hltcapywvh psgksapwrd hwmqgvyfpp ievdvdrgqe
      361 lylvacrdqy smwfatslsd cteappvpkc lcslhaafsr srigmlndas rnakytaalq
      421 klinresvcl cigsfnmlpl malklgakmv yviepdermr svleeyasvn qikgiqflsv
      481 khnggdsaer sseddsveka nlllsepffa tsllpwhnlk fwfqrfslsy llapsaitmp
      541 rtcalygvav efhdlwkisa pvgategftl qafdsliqes rsisddpvep hplweypcta
      601 ltnkfqlmyt hlqvkakesp lllsgstsfe srglcngvvl wtdfdldgyn vvtsgpseti
      661 ipgktitwdr fsrqgvqfft pgqknatkll wklcfdlags vtfdfkfetr dqa
//
LOCUS       XP_077503444             683 aa            linear   INV 25-NOV-2025
DEFINITION  protein arginine N-methyltransferase 7-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503444
VERSION     XP_077503444.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647318.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..683
                     /product="protein arginine N-methyltransferase 7-like
                     isoform X2"
                     /calculated_mol_wt=76165
     Region          38..>194
                     /region_name="COG4076"
                     /note="Predicted RNA methylase [General function
                     prediction only]"
                     /db_xref="CDD:443253"
     Region          375..>521
                     /region_name="COG4076"
                     /note="Predicted RNA methylase [General function
                     prediction only]"
                     /db_xref="CDD:443253"
     CDS             1..683
                     /gene="LOC144113923"
                     /coded_by="XM_077647318.1:155..2206"
                     /db_xref="GeneID:144113923"
ORIGIN      
        1 madsyyvenp mtgkdewktq aveydhhqdv arsafgdmlh dgernrkyhi alceaidavr
       61 argekvvvld lgtgsgilam maakrgadvv yaceafepva qcaeqvirdn hlgdrihvir
      121 kkstsiqvgp gkdmeeranv lvtevfdtel igegaietfv halkellepa cvvvphaatm
      181 yaqvvhspfl rshhtlssvc mtpelqikvp qsvqtcagts avhdiqlsql qesdflpite
      241 plpifrydft daktmpyedf tvsvtkatnr gtghavlmww aldmncsgsi hltcapywvh
      301 psgksapwrd hwmqgvyfpp ievdvdrgqe lylvacrdqy smwfatslsd cteappvpkc
      361 lcslhaafsr srigmlndas rnakytaalq klinresvcl cigsfnmlpl malklgakmv
      421 yviepdermr svleeyasvn qikgiqflsv khnggdsaer sseddsveka nlllsepffa
      481 tsllpwhnlk fwfqrfslsy llapsaitmp rtcalygvav efhdlwkisa pvgategftl
      541 qafdsliqes rsisddpvep hplweypcta ltnkfqlmyt hlqvkakesp lllsgstsfe
      601 srglcngvvl wtdfdldgyn vvtsgpseti ipgktitwdr fsrqgvqfft pgqknatkll
      661 wklcfdlags vtfdfkfetr dqa
//
LOCUS       XP_077503445            1577 aa            linear   INV 25-NOV-2025
DEFINITION  mutagen-sensitive 101 [Amblyomma americanum].
ACCESSION   XP_077503445
VERSION     XP_077503445.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647319.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1577
                     /product="mutagen-sensitive 101"
                     /calculated_mol_wt=172379
     Region          110..180
                     /region_name="BRCT"
                     /note="C-terminal domain of the breast cancer suppressor
                     protein (BRCA1) and related domains; cl00038"
                     /db_xref="CDD:469589"
     Region          199..275
                     /region_name="BRCT_TopBP1_rpt2_like"
                     /note="second BRCT domain of DNA topoisomerase 2-binding
                     protein 1 (TopBP1) and similar proteins; cd17731"
                     /db_xref="CDD:349363"
     Site            order(208..212,216,232..235,237,250..251,254,258..259)
                     /site_type="other"
                     /note="Crb2 interaction site [polypeptide binding]"
                     /db_xref="CDD:349363"
     Region          374..457
                     /region_name="BRCT_TopBP1_rpt3"
                     /note="third BRCT domain of DNA topoisomerase 2-binding
                     protein 1 (TopBP1) and similar proteins; cd17718"
                     /db_xref="CDD:349350"
     Region          578..667
                     /region_name="BRCT"
                     /note="C-terminal domain of the breast cancer suppressor
                     protein (BRCA1) and related domains; cl00038"
                     /db_xref="CDD:469589"
     Region          676..766
                     /region_name="BRCT"
                     /note="C-terminal domain of the breast cancer suppressor
                     protein (BRCA1) and related domains; cl00038"
                     /db_xref="CDD:469589"
     Region          1044..1118
                     /region_name="BRCT_TopBP1_rpt6"
                     /note="sixth BRCT domain of DNA topoisomerase 2-binding
                     protein 1 (TopBP1) and similar proteins; cd17727"
                     /db_xref="CDD:349359"
     Region          1311..1384
                     /region_name="BRCT_TopBP1_rpt7"
                     /note="seventh BRCT domain of DNA topoisomerase 2-binding
                     protein 1; cd17738"
                     /db_xref="CDD:349370"
     Site            order(1315..1319,1323,1354,1356..1360)
                     /site_type="other"
                     /note="BACH1 interaction site [polypeptide binding]"
                     /db_xref="CDD:349370"
     Region          1444..1522
                     /region_name="BRCT_TopBP1_rpt8"
                     /note="eighth (C-terminal) BRCT domain of DNA
                     topoisomerase 2-binding protein 1; cd17728"
                     /db_xref="CDD:349360"
     CDS             1..1577
                     /gene="mus101"
                     /coded_by="XM_077647319.1:212..4945"
                     /db_xref="GeneID:144113926"
ORIGIN      
        1 mdsipdgkll fvlregqtaa daspamqaaf kaaglermep switacaclt rkrrdptevf
       61 vcdpfegiaf ehlrqiqarv vgplcvsmai qvqeplprrp epvfslafrg lvitcsalcs
      121 eerewiktqv elmggsvvap ltasvthvva gdvgskkyhv aanrkipvmq lswvqtfwee
      181 eqhklahass layshlrlpa fkglvitvsq vpaserkelq alvesnggsy sgqlhckttt
      241 hvalleaage kynharlwkl hcvhirwlye sahagyalde slyaleppsv ckkstprsst
      301 sqdvtlppwd csaiattest rvnettrane gtvtfeanfk hslggvnesa gtlceaksrd
      361 pvddidvdkl ssccgqfldg ccvllwgfaq eklekmrriv nvcggvrlde ykeevthvvv
      421 aegphltpei mkiikrhggt pyvvsaqwfv essalgklqk inkyllvelh shvegaeips
      481 dclannmdtq qepavlehgn pgpsdftdim eqyrtsnnvs mnardnrsts hseksmgpat
      541 gdnvekmnat lysfeasakn gslserpleq cepadalftg mtfrlhdfse hdqlilkeai
      601 tqnggkiwed gyvckdeqkf ieivrlvids tkdyssyegl mityywleac lqeesllnfd
      661 edalfrpflk tpngdplrgc visfsqystp ereclaylav smgaacqdsl vrsvsrkqqs
      721 kilpnthlia repegskyea avkwglpvvt kewlvasarq gkkmnedgfl lndaarqpli
      781 gkpasemtsc qeqaqfvnap nqnepctdkp evsfidqdiv plqpdkeshk khyeqaencc
      841 evqphrgstg qtweahgars sisstpsvck kpesftlnep gfadectdna vyerskiaaa
      901 epakafctpg grrkistgga stpgsflksq rirellsesh nsmnssvtsp gassddesfq
      961 qtiggsyrpk lnitgleedf aaqenrnasr ksrpfslgkq varnlelnve rfcanediag
     1021 lldspspglq tvpldgarsg srplagavvc vskrlshvqk elgdivcelg gefipayaeh
     1081 cthlvhqgkp geplprdvlr areqgkklvs sgwvyackgs gswldeadfp sahderlslv
     1141 gyvstvrvpk qrsqqsalsq lgspkhirsp heengspkkp pstvvqprrl tesqqmindq
     1201 ldelmnaaka agqrrslrpp pvpviaspih pvltatdsik rsvitslplp dsesqfvgvt
     1261 wddptrrlem arlagklaev eppsqpateq spegehdgfe eaepvmdppv kvfvlsgfsd
     1321 eqkiryasii sqlkgvllts kaynpemthl vlvqalkser ylgalaagkf ilhaayldes
     1381 akagkfldee efewggpmte nclmnlstas tkshkskgqp afaprrwrqh iaqsagckga
     1441 fsewrvviya saskeaayvn vltaggativ ppttvcpgls rgevthalfd agmvkgvqlg
     1501 llvsagalcl kaeymaaflv ddpappperf yipevvklld csistdvnad tssaskrsaa
     1561 stssrgslal gakrrkq
//
LOCUS       XP_077503446            1577 aa            linear   INV 25-NOV-2025
DEFINITION  mutagen-sensitive 101 [Amblyomma americanum].
ACCESSION   XP_077503446
VERSION     XP_077503446.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647320.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1577
                     /product="mutagen-sensitive 101"
                     /calculated_mol_wt=172379
     Region          110..180
                     /region_name="BRCT"
                     /note="C-terminal domain of the breast cancer suppressor
                     protein (BRCA1) and related domains; cl00038"
                     /db_xref="CDD:469589"
     Region          199..275
                     /region_name="BRCT_TopBP1_rpt2_like"
                     /note="second BRCT domain of DNA topoisomerase 2-binding
                     protein 1 (TopBP1) and similar proteins; cd17731"
                     /db_xref="CDD:349363"
     Site            order(208..212,216,232..235,237,250..251,254,258..259)
                     /site_type="other"
                     /note="Crb2 interaction site [polypeptide binding]"
                     /db_xref="CDD:349363"
     Region          374..457
                     /region_name="BRCT_TopBP1_rpt3"
                     /note="third BRCT domain of DNA topoisomerase 2-binding
                     protein 1 (TopBP1) and similar proteins; cd17718"
                     /db_xref="CDD:349350"
     Region          578..667
                     /region_name="BRCT"
                     /note="C-terminal domain of the breast cancer suppressor
                     protein (BRCA1) and related domains; cl00038"
                     /db_xref="CDD:469589"
     Region          676..766
                     /region_name="BRCT"
                     /note="C-terminal domain of the breast cancer suppressor
                     protein (BRCA1) and related domains; cl00038"
                     /db_xref="CDD:469589"
     Region          1044..1118
                     /region_name="BRCT_TopBP1_rpt6"
                     /note="sixth BRCT domain of DNA topoisomerase 2-binding
                     protein 1 (TopBP1) and similar proteins; cd17727"
                     /db_xref="CDD:349359"
     Region          1311..1384
                     /region_name="BRCT_TopBP1_rpt7"
                     /note="seventh BRCT domain of DNA topoisomerase 2-binding
                     protein 1; cd17738"
                     /db_xref="CDD:349370"
     Site            order(1315..1319,1323,1354,1356..1360)
                     /site_type="other"
                     /note="BACH1 interaction site [polypeptide binding]"
                     /db_xref="CDD:349370"
     Region          1444..1522
                     /region_name="BRCT_TopBP1_rpt8"
                     /note="eighth (C-terminal) BRCT domain of DNA
                     topoisomerase 2-binding protein 1; cd17728"
                     /db_xref="CDD:349360"
     CDS             1..1577
                     /gene="mus101"
                     /coded_by="XM_077647320.1:132..4865"
                     /db_xref="GeneID:144113926"
ORIGIN      
        1 mdsipdgkll fvlregqtaa daspamqaaf kaaglermep switacaclt rkrrdptevf
       61 vcdpfegiaf ehlrqiqarv vgplcvsmai qvqeplprrp epvfslafrg lvitcsalcs
      121 eerewiktqv elmggsvvap ltasvthvva gdvgskkyhv aanrkipvmq lswvqtfwee
      181 eqhklahass layshlrlpa fkglvitvsq vpaserkelq alvesnggsy sgqlhckttt
      241 hvalleaage kynharlwkl hcvhirwlye sahagyalde slyaleppsv ckkstprsst
      301 sqdvtlppwd csaiattest rvnettrane gtvtfeanfk hslggvnesa gtlceaksrd
      361 pvddidvdkl ssccgqfldg ccvllwgfaq eklekmrriv nvcggvrlde ykeevthvvv
      421 aegphltpei mkiikrhggt pyvvsaqwfv essalgklqk inkyllvelh shvegaeips
      481 dclannmdtq qepavlehgn pgpsdftdim eqyrtsnnvs mnardnrsts hseksmgpat
      541 gdnvekmnat lysfeasakn gslserpleq cepadalftg mtfrlhdfse hdqlilkeai
      601 tqnggkiwed gyvckdeqkf ieivrlvids tkdyssyegl mityywleac lqeesllnfd
      661 edalfrpflk tpngdplrgc visfsqystp ereclaylav smgaacqdsl vrsvsrkqqs
      721 kilpnthlia repegskyea avkwglpvvt kewlvasarq gkkmnedgfl lndaarqpli
      781 gkpasemtsc qeqaqfvnap nqnepctdkp evsfidqdiv plqpdkeshk khyeqaencc
      841 evqphrgstg qtweahgars sisstpsvck kpesftlnep gfadectdna vyerskiaaa
      901 epakafctpg grrkistgga stpgsflksq rirellsesh nsmnssvtsp gassddesfq
      961 qtiggsyrpk lnitgleedf aaqenrnasr ksrpfslgkq varnlelnve rfcanediag
     1021 lldspspglq tvpldgarsg srplagavvc vskrlshvqk elgdivcelg gefipayaeh
     1081 cthlvhqgkp geplprdvlr areqgkklvs sgwvyackgs gswldeadfp sahderlslv
     1141 gyvstvrvpk qrsqqsalsq lgspkhirsp heengspkkp pstvvqprrl tesqqmindq
     1201 ldelmnaaka agqrrslrpp pvpviaspih pvltatdsik rsvitslplp dsesqfvgvt
     1261 wddptrrlem arlagklaev eppsqpateq spegehdgfe eaepvmdppv kvfvlsgfsd
     1321 eqkiryasii sqlkgvllts kaynpemthl vlvqalkser ylgalaagkf ilhaayldes
     1381 akagkfldee efewggpmte nclmnlstas tkshkskgqp afaprrwrqh iaqsagckga
     1441 fsewrvviya saskeaayvn vltaggativ ppttvcpgls rgevthalfd agmvkgvqlg
     1501 llvsagalcl kaeymaaflv ddpappperf yipevvklld csistdvnad tssaskrsaa
     1561 stssrgslal gakrrkq
//
LOCUS       XP_077503447             350 aa            linear   INV 25-NOV-2025
DEFINITION  D-aspartate oxidase-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503447
VERSION     XP_077503447.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647321.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..350
                     /product="D-aspartate oxidase-like isoform X1"
                     /calculated_mol_wt=38551
     Region          26..334
                     /region_name="DAO"
                     /note="FAD dependent oxidoreductase; pfam01266"
                     /db_xref="CDD:426168"
     CDS             1..350
                     /gene="LOC144113927"
                     /coded_by="XM_077647321.1:153..1205"
                     /db_xref="GeneID:144113927"
ORIGIN      
        1 mpahqsqvgs aarkkrvavv gagvvglsta lciqesipgt svtviadrfi edtlsfgaag
       61 ffrpdenigp tleitrewyr ttfqhyeall rsntaeaagi kklsgyalss snpeklvngt
      121 mkelcgglrp itkgelknfp skykhgifyn siladprkyl kwltdkiisn ggqlknqtik
      181 dlrdleefcv vvncsglrak eltqdplltp vrgqvikvla pwvtqfyyan gcyilpgtey
      241 vtlggtkqlg dwniqvsqhd rdyiwsicta vlpslkdckv iedwvglrpf rqpirieaev
      301 lgcgknkckv vhnyghgahg intswgtalh atrlvyeilq pqtasivakl
//
LOCUS       XP_077503448             350 aa            linear   INV 25-NOV-2025
DEFINITION  D-aspartate oxidase-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503448
VERSION     XP_077503448.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647322.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..350
                     /product="D-aspartate oxidase-like isoform X1"
                     /calculated_mol_wt=38551
     Region          26..334
                     /region_name="DAO"
                     /note="FAD dependent oxidoreductase; pfam01266"
                     /db_xref="CDD:426168"
     CDS             1..350
                     /gene="LOC144113927"
                     /coded_by="XM_077647322.1:147..1199"
                     /db_xref="GeneID:144113927"
ORIGIN      
        1 mpahqsqvgs aarkkrvavv gagvvglsta lciqesipgt svtviadrfi edtlsfgaag
       61 ffrpdenigp tleitrewyr ttfqhyeall rsntaeaagi kklsgyalss snpeklvngt
      121 mkelcgglrp itkgelknfp skykhgifyn siladprkyl kwltdkiisn ggqlknqtik
      181 dlrdleefcv vvncsglrak eltqdplltp vrgqvikvla pwvtqfyyan gcyilpgtey
      241 vtlggtkqlg dwniqvsqhd rdyiwsicta vlpslkdckv iedwvglrpf rqpirieaev
      301 lgcgknkckv vhnyghgahg intswgtalh atrlvyeilq pqtasivakl
//
LOCUS       XP_077503450             324 aa            linear   INV 25-NOV-2025
DEFINITION  D-aspartate oxidase-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503450
VERSION     XP_077503450.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647324.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..324
                     /product="D-aspartate oxidase-like isoform X2"
                     /calculated_mol_wt=35763
     Region          26..291
                     /region_name="DAO"
                     /note="FAD dependent oxidoreductase; pfam01266"
                     /db_xref="CDD:426168"
     CDS             1..324
                     /gene="LOC144113927"
                     /coded_by="XM_077647324.1:153..1127"
                     /db_xref="GeneID:144113927"
ORIGIN      
        1 mpahqsqvgs aarkkrvavv gagvvglsta lciqesipgt svtviadrfi edtlsfgaag
       61 ffrpdenigp tleitrewyr ttfqhyeall rsntaeaagi kklsgyalss snpeklvngt
      121 mkelcgglrp itkgelknfp skykhgifyn siladprkyl kwltdkiisn ggqlknqtik
      181 dlrdleefcv vvncsglrak eltqdplltp vrgqvikvla pwvtqfyyan gcyilpgtey
      241 vtlggtkqlg dwniqvsqhd rdyiwsicta vlpslkdckv iedwvglrpf rqpirieaev
      301 lgcgknkckf kggqlcvkkv lvaf
//
LOCUS       XP_077503451             965 aa            linear   INV 25-NOV-2025
DEFINITION  secretory pathway calcium atpase isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503451
VERSION     XP_077503451.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647325.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..965
                     /product="secretory pathway calcium atpase isoform X1"
                     /calculated_mol_wt=105497
     Region          104..944
                     /region_name="P-type_ATPase_SPCA"
                     /note="golgi-associated secretory pathway Ca(2+) transport
                     ATPases, similar to human ATPase secretory pathway Ca(2+)
                     transporting 1/hSPCA1 and Saccharomyces cerevisiae
                     Ca(2+)/Mn(2+)-transporting P-type ATPase, Pmr1p; cd02085"
                     /db_xref="CDD:319779"
     Site            order(399..401,503,528..530,573,618..620,667,670,673,692,
                     695)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319779"
     Site            order(753,757..758,760..761,786,789..790)
                     /site_type="other"
                     /note="putative cation binding site [ion binding]"
                     /db_xref="CDD:319779"
     CDS             1..965
                     /gene="SPoCk"
                     /coded_by="XM_077647325.1:130..3027"
                     /db_xref="GeneID:144113928"
ORIGIN      
        1 masdvdvlsm sesltqvsfr krkivpkpwa asrrllqgtt lirkeagggs asdsdndaea
       61 lplahgaaeg mhteeacqlp fevvaerlgt dlrrglswne ahrrlltvga nefevrqeep
      121 lckkyldqfk nplivlllas avvsvcmqqf ddafsitaai iivvtvafvq eyrsekslee
      181 lnklvpptch clrggqaetf laknlvpgdi iilnvgdrvp adirlfsavd lmidessftg
      241 etepaakvta plekagngia skrnlafmgt lvrygngrgi vintgeksef gdifkmmqae
      301 eapktplqrs mdtlgkqlsl ysfgiiglim llgwiqgrpl lemfnigvsl avaaipeglp
      361 ivvtvtlalg vmrmakrkai ikklptvetl gcvnvvcsdk tgtltknemt vcsmmtsely
      421 haevtgvgyn adghviltep gerevqmesm rlllecgsic nnadilgsel rgqptegall
      481 aaahkmgiyd vrnhyvrlqe ipfssehkmm avkvipkfgg eqeqyfvkga lekilahcth
      541 ysghgmplpi ttertaqyik eaqfmgrkgl rvlslasgtn lnqlkflglv gildpprpgv
      601 rdsietlhss gvnvkmltgd seetactvaa rlglhavgsv vlsgdqldam tdselqriig
      661 svsvfyrtgp ghklrivkal qrnhyivgmt gdgvndgval kkadigiamg kigtdvckea
      721 admilvdddf ftimaaieeg kgifynirnf vtfqlstsia alslialstl mkipnplnam
      781 qilwiniimd gppaqslgve pvdhdvlkqp prntkqpmit rdlifnvvis sliiilgtlf
      841 ifkkemsdqi vtprdttmtf tcfvffdmfn alscrsqtks aftiglfsnr mfllavafsv
      901 igqmlviyfp plqrvfqtea ltaldicllv gvsstvfivs elkkffersi srrvrkkqff
      961 gsdfv
//
LOCUS       XP_077503452             949 aa            linear   INV 25-NOV-2025
DEFINITION  secretory pathway calcium atpase isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503452
VERSION     XP_077503452.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647326.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..949
                     /product="secretory pathway calcium atpase isoform X2"
                     /calculated_mol_wt=103734
     Region          88..928
                     /region_name="P-type_ATPase_SPCA"
                     /note="golgi-associated secretory pathway Ca(2+) transport
                     ATPases, similar to human ATPase secretory pathway Ca(2+)
                     transporting 1/hSPCA1 and Saccharomyces cerevisiae
                     Ca(2+)/Mn(2+)-transporting P-type ATPase, Pmr1p; cd02085"
                     /db_xref="CDD:319779"
     Site            order(383..385,487,512..514,557,602..604,651,654,657,676,
                     679)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319779"
     Site            order(737,741..742,744..745,770,773..774)
                     /site_type="other"
                     /note="putative cation binding site [ion binding]"
                     /db_xref="CDD:319779"
     CDS             1..949
                     /gene="SPoCk"
                     /coded_by="XM_077647326.1:192..3041"
                     /db_xref="GeneID:144113928"
ORIGIN      
        1 mkttyeklpt evhhqgsptr qnthhghsea gggsasdsdn daealplahg aaegmhteea
       61 cqlpfevvae rlgtdlrrgl swneahrrll tvganefevr qeeplckkyl dqfknplivl
      121 llasavvsvc mqqfddafsi taaiiivvtv afvqeyrsek sleelnklvp ptchclrggq
      181 aetflaknlv pgdiiilnvg drvpadirlf savdlmides sftgetepaa kvtaplekag
      241 ngiaskrnla fmgtlvrygn grgivintge ksefgdifkm mqaeeapktp lqrsmdtlgk
      301 qlslysfgii glimllgwiq grpllemfni gvslavaaip eglpivvtvt lalgvmrmak
      361 rkaiikklpt vetlgcvnvv csdktgtltk nemtvcsmmt selyhaevtg vgynadghvi
      421 ltepgerevq mesmrlllec gsicnnadil gselrgqpte gallaaahkm giydvrnhyv
      481 rlqeipfsse hkmmavkvip kfggeqeqyf vkgalekila hcthysghgm plpitterta
      541 qyikeaqfmg rkglrvlsla sgtnlnqlkf lglvgildpp rpgvrdsiet lhssgvnvkm
      601 ltgdseetac tvaarlglha vgsvvlsgdq ldamtdselq riigsvsvfy rtgpghklri
      661 vkalqrnhyi vgmtgdgvnd gvalkkadig iamgkigtdv ckeaadmilv dddfftimaa
      721 ieegkgifyn irnfvtfqls tsiaalslia lstlmkipnp lnamqilwin iimdgppaqs
      781 lgvepvdhdv lkqpprntkq pmitrdlifn vvissliiil gtlfifkkem sdqivtprdt
      841 tmtftcfvff dmfnalscrs qtksaftigl fsnrmfllav afsvigqmlv iyfpplqrvf
      901 qtealtaldi cllvgvsstv fivselkkff ersisrrvrk kqffgsdfv
//
LOCUS       XP_077503453             895 aa            linear   INV 25-NOV-2025
DEFINITION  secretory pathway calcium atpase isoform X3 [Amblyomma americanum].
ACCESSION   XP_077503453
VERSION     XP_077503453.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647327.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..895
                     /product="secretory pathway calcium atpase isoform X3"
                     /calculated_mol_wt=98161
     Region          34..874
                     /region_name="P-type_ATPase_SPCA"
                     /note="golgi-associated secretory pathway Ca(2+) transport
                     ATPases, similar to human ATPase secretory pathway Ca(2+)
                     transporting 1/hSPCA1 and Saccharomyces cerevisiae
                     Ca(2+)/Mn(2+)-transporting P-type ATPase, Pmr1p; cd02085"
                     /db_xref="CDD:319779"
     Site            order(329..331,433,458..460,503,548..550,597,600,603,622,
                     625)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319779"
     Site            order(683,687..688,690..691,716,719..720)
                     /site_type="other"
                     /note="putative cation binding site [ion binding]"
                     /db_xref="CDD:319779"
     CDS             1..895
                     /gene="SPoCk"
                     /coded_by="XM_077647327.1:295..2982"
                     /db_xref="GeneID:144113928"
ORIGIN      
        1 mhteeacqlp fevvaerlgt dlrrglswne ahrrlltvga nefevrqeep lckkyldqfk
       61 nplivlllas avvsvcmqqf ddafsitaai iivvtvafvq eyrsekslee lnklvpptch
      121 clrggqaetf laknlvpgdi iilnvgdrvp adirlfsavd lmidessftg etepaakvta
      181 plekagngia skrnlafmgt lvrygngrgi vintgeksef gdifkmmqae eapktplqrs
      241 mdtlgkqlsl ysfgiiglim llgwiqgrpl lemfnigvsl avaaipeglp ivvtvtlalg
      301 vmrmakrkai ikklptvetl gcvnvvcsdk tgtltknemt vcsmmtsely haevtgvgyn
      361 adghviltep gerevqmesm rlllecgsic nnadilgsel rgqptegall aaahkmgiyd
      421 vrnhyvrlqe ipfssehkmm avkvipkfgg eqeqyfvkga lekilahcth ysghgmplpi
      481 ttertaqyik eaqfmgrkgl rvlslasgtn lnqlkflglv gildpprpgv rdsietlhss
      541 gvnvkmltgd seetactvaa rlglhavgsv vlsgdqldam tdselqriig svsvfyrtgp
      601 ghklrivkal qrnhyivgmt gdgvndgval kkadigiamg kigtdvckea admilvdddf
      661 ftimaaieeg kgifynirnf vtfqlstsia alslialstl mkipnplnam qilwiniimd
      721 gppaqslgve pvdhdvlkqp prntkqpmit rdlifnvvis sliiilgtlf ifkkemsdqi
      781 vtprdttmtf tcfvffdmfn alscrsqtks aftiglfsnr mfllavafsv igqmlviyfp
      841 plqrvfqtea ltaldicllv gvsstvfivs elkkffersi srrvrkkqff gsdfv
//
LOCUS       XP_077503454             566 aa            linear   INV 25-NOV-2025
DEFINITION  kelch like family member 18 [Amblyomma americanum].
ACCESSION   XP_077503454
VERSION     XP_077503454.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647328.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..566
                     /product="kelch like family member 18"
                     /calculated_mol_wt=62181
     Region          13..128
                     /region_name="BTB_POZ_KLHL18"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in Kelch-like
                     protein 18 (KLHL18); cd18247"
                     /db_xref="CDD:349556"
     Region          129..264
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     Region          283..330
                     /region_name="Kelch"
                     /note="Kelch domain; smart00612"
                     /db_xref="CDD:128874"
     Region          308..551
                     /region_name="NanM"
                     /note="N-acetylneuraminic acid mutarotase [Cell
                     wall/membrane/envelope biogenesis]; COG3055"
                     /db_xref="CDD:442289"
     Region          320..363
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          367..411
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          414..459
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          461..506
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          508..551
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     CDS             1..566
                     /gene="KLHL18"
                     /coded_by="XM_077647328.1:154..1854"
                     /db_xref="GeneID:144113929"
ORIGIN      
        1 maadecymfs qsdlpassfg lieeirrqgk lcditlkvdd eiftahrivl astipyfyam
       61 fthdmveskq tevtihgida salealinfa ysgkvnitaa nvqsllvgas flqllkvrea
      121 cseflvkrlh pnnvlgiraf adtlgcpalv eatnkfiqmh fldvcqsfef lalpledame
      181 iigwnqlyvv seeqvfeavm swvlhdregr gsllpdilah vrlpllspqf ltdrvaaepl
      241 vrgchrcrdl ldeardyllm perrpllqgf rtrprccpdv aghiyavggl tkagdsqstv
      301 evydpklghw qvaeamsmtr srvgvavlgg klyaiggyng ierlrtvevf spesriwsrv
      361 asmnckrsav gaavlhdkly vcggydgvss lntvecynpe rnewtmvtsm skhrsaagvv
      421 afdghiyalg ghdglsifgs verydvqtgq wspmpsmlth rcrlgvavlq gkiyvcggyd
      481 gakflqttea fdpvtqqwqf vapmnvtrsr vslvancgrl favggydgvs nlstvevydp
      541 eanqwtpias mcaheggvgv gvlppl
//
LOCUS       XP_077503455             566 aa            linear   INV 25-NOV-2025
DEFINITION  kelch like family member 18 [Amblyomma americanum].
ACCESSION   XP_077503455
VERSION     XP_077503455.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647329.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..566
                     /product="kelch like family member 18"
                     /calculated_mol_wt=62181
     Region          13..128
                     /region_name="BTB_POZ_KLHL18"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in Kelch-like
                     protein 18 (KLHL18); cd18247"
                     /db_xref="CDD:349556"
     Region          129..264
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     Region          283..330
                     /region_name="Kelch"
                     /note="Kelch domain; smart00612"
                     /db_xref="CDD:128874"
     Region          308..551
                     /region_name="NanM"
                     /note="N-acetylneuraminic acid mutarotase [Cell
                     wall/membrane/envelope biogenesis]; COG3055"
                     /db_xref="CDD:442289"
     Region          320..363
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          367..411
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          414..459
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          461..506
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          508..551
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     CDS             1..566
                     /gene="KLHL18"
                     /coded_by="XM_077647329.1:163..1863"
                     /db_xref="GeneID:144113929"
ORIGIN      
        1 maadecymfs qsdlpassfg lieeirrqgk lcditlkvdd eiftahrivl astipyfyam
       61 fthdmveskq tevtihgida salealinfa ysgkvnitaa nvqsllvgas flqllkvrea
      121 cseflvkrlh pnnvlgiraf adtlgcpalv eatnkfiqmh fldvcqsfef lalpledame
      181 iigwnqlyvv seeqvfeavm swvlhdregr gsllpdilah vrlpllspqf ltdrvaaepl
      241 vrgchrcrdl ldeardyllm perrpllqgf rtrprccpdv aghiyavggl tkagdsqstv
      301 evydpklghw qvaeamsmtr srvgvavlgg klyaiggyng ierlrtvevf spesriwsrv
      361 asmnckrsav gaavlhdkly vcggydgvss lntvecynpe rnewtmvtsm skhrsaagvv
      421 afdghiyalg ghdglsifgs verydvqtgq wspmpsmlth rcrlgvavlq gkiyvcggyd
      481 gakflqttea fdpvtqqwqf vapmnvtrsr vslvancgrl favggydgvs nlstvevydp
      541 eanqwtpias mcaheggvgv gvlppl
//
LOCUS       XP_077503456             396 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113930 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503456
VERSION     XP_077503456.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647330.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..396
                     /product="uncharacterized protein LOC144113930 isoform X1"
                     /calculated_mol_wt=45292
     Region          23..235
                     /region_name="HAUS6_N"
                     /note="HAUS augmin-like complex subunit 6 N-terminus;
                     pfam14661"
                     /db_xref="CDD:464244"
     Region          171..>348
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     CDS             1..396
                     /gene="LOC144113930"
                     /coded_by="XM_077647330.1:30..1220"
                     /db_xref="GeneID:144113930"
ORIGIN      
        1 mvpllsrefc gvvlnffrme dvllicldal hfkppddtkl lqehfrgrqa patvrqvlhf
       61 lyytydkkav esrmrniwpv ktgesdkrfr eeitqwistl qgdelatpyi qefsaalllr
      121 pgtpqgfkfl aslsvfvlhy klkdsvhfss pfspaerpaw klemvkkati qtrsqnaafk
      181 eeldafesek akmqsllekk rsdlelsinh lqaqvqsfes ddtlmslekl crklraeerd
      241 skktleiine avetfsrskr vidlssltdk qskflsssgl vdvvgtfkdc telakklsaq
      301 fvkegctqqd leavklrvaa lratlrrlqe qretakamka eidqrlsagc kkpfvvlndk
      361 didemcrpaa qpatepdvee fvnellavhr rqnrnq
//
LOCUS       XP_077503457             378 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113930 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503457
VERSION     XP_077503457.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647331.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..378
                     /product="uncharacterized protein LOC144113930 isoform X2"
                     /calculated_mol_wt=43183
     Region          5..217
                     /region_name="HAUS6_N"
                     /note="HAUS augmin-like complex subunit 6 N-terminus;
                     pfam14661"
                     /db_xref="CDD:464244"
     Region          153..>330
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     CDS             1..378
                     /gene="LOC144113930"
                     /coded_by="XM_077647331.1:141..1277"
                     /db_xref="GeneID:144113930"
ORIGIN      
        1 medvllicld alhfkppddt kllqehfrgr qapatvrqvl hflyytydkk avesrmrniw
       61 pvktgesdkr freeitqwis tlqgdelatp yiqefsaall lrpgtpqgfk flaslsvfvl
      121 hyklkdsvhf sspfspaerp awklemvkka tiqtrsqnaa fkeeldafes ekakmqslle
      181 kkrsdlelsi nhlqaqvqsf esddtlmsle klcrklraee rdskktleii neavetfsrs
      241 krvidlsslt dkqskflsss glvdvvgtfk dctelakkls aqfvkegctq qdleavklrv
      301 aalratlrrl qeqretakam kaeidqrlsa gckkpfvvln dkdidemcrp aaqpatepdv
      361 eefvnellav hrrqnrnq
//
LOCUS       XP_077503458             378 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113930 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503458
VERSION     XP_077503458.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647332.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..378
                     /product="uncharacterized protein LOC144113930 isoform X2"
                     /calculated_mol_wt=43183
     Region          5..217
                     /region_name="HAUS6_N"
                     /note="HAUS augmin-like complex subunit 6 N-terminus;
                     pfam14661"
                     /db_xref="CDD:464244"
     Region          153..>330
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     CDS             1..378
                     /gene="LOC144113930"
                     /coded_by="XM_077647332.1:116..1252"
                     /db_xref="GeneID:144113930"
ORIGIN      
        1 medvllicld alhfkppddt kllqehfrgr qapatvrqvl hflyytydkk avesrmrniw
       61 pvktgesdkr freeitqwis tlqgdelatp yiqefsaall lrpgtpqgfk flaslsvfvl
      121 hyklkdsvhf sspfspaerp awklemvkka tiqtrsqnaa fkeeldafes ekakmqslle
      181 kkrsdlelsi nhlqaqvqsf esddtlmsle klcrklraee rdskktleii neavetfsrs
      241 krvidlsslt dkqskflsss glvdvvgtfk dctelakkls aqfvkegctq qdleavklrv
      301 aalratlrrl qeqretakam kaeidqrlsa gckkpfvvln dkdidemcrp aaqpatepdv
      361 eefvnellav hrrqnrnq
//
LOCUS       XP_077503459             135 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113932 [Amblyomma americanum].
ACCESSION   XP_077503459
VERSION     XP_077503459.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647333.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..135
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..135
                     /product="uncharacterized protein LOC144113932"
                     /calculated_mol_wt=14779
     CDS             1..135
                     /gene="LOC144113932"
                     /coded_by="XM_077647333.1:14..421"
                     /db_xref="GeneID:144113932"
ORIGIN      
        1 mlvlidgvet rayalldehg thmenddgfl yetegglris vlrveepirn tppptvpavv
       61 edgsrlapsp lrqttppvvw qptpspgpsc yspppsghge rdawndrksr flitkykkpr
      121 ialaervalg nsprc
//
LOCUS       XP_077503460             659 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113933 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503460
VERSION     XP_077503460.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647334.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..659
                     /product="uncharacterized protein LOC144113933 isoform X1"
                     /calculated_mol_wt=71098
     Region          14..112
                     /region_name="HSF_DNA-bind"
                     /note="HSF-type DNA-binding; pfam00447"
                     /db_xref="CDD:459813"
     Region          <489..595
                     /region_name="Vert_HS_TF"
                     /note="Vertebrate heat shock transcription factor;
                     pfam06546"
                     /db_xref="CDD:461944"
     CDS             1..659
                     /gene="LOC144113933"
                     /coded_by="XM_077647334.1:152..2131"
                     /db_xref="GeneID:144113933"
ORIGIN      
        1 mhttefggss vpaflvklwk lvedeecndl iswnsngqsf iihnqtqfak ellplyfkhn
       61 nmaslirqln iygfrkvtnt dqglrsdree ieffhsffir gqecllefik rkvptsragg
      121 aatddgrdhs eafkgllsdv gsiqgrqehm dqsladmkre ngalwrevar lrqkhmkqqq
      181 iigklieflv tmvqanrnit vkrkvpfmlh dtsnpkmprl tkaafvtnyq vnspadslvs
      241 egpvihdvtd lmedlgetsg sivcepltag qspreievsr patpvqllas paatrdldtg
      301 pivasslnaa daieplvlld plpddqssyv apcdieevvv nssepspkep stasskslvv
      361 ptvktlqvat patsavkqtt pldsvsyeaf tssnlfgdee ptllgdrhaa ngtsdllssc
      421 vmlegipmfv gdsaasepas plgltvpats hasegspkhk edsgaasvsp dmqvalqdka
      481 fgntkvfaeh ldsidydldw lqdqlmtgal nldtstlmgv cpdwsgllsg slklfssedp
      541 ltsclgdldt egrfssdtig nevvqytpsl ldlgieedss feavnellls ddevpsslct
      601 tsiasgesis rpnslvasgk csglrqtsss vraavqtvpr svtkkgtlss kkrrgsskk
//
LOCUS       XP_077503461             655 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113933 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503461
VERSION     XP_077503461.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647335.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..655
                     /product="uncharacterized protein LOC144113933 isoform X2"
                     /calculated_mol_wt=70620
     Region          14..112
                     /region_name="HSF_DNA-bind"
                     /note="HSF-type DNA-binding; pfam00447"
                     /db_xref="CDD:459813"
     Region          <485..591
                     /region_name="Vert_HS_TF"
                     /note="Vertebrate heat shock transcription factor;
                     pfam06546"
                     /db_xref="CDD:461944"
     CDS             1..655
                     /gene="LOC144113933"
                     /coded_by="XM_077647335.1:149..2116"
                     /db_xref="GeneID:144113933"
ORIGIN      
        1 mhttefggss vpaflvklwk lvedeecndl iswnsngqsf iihnqtqfak ellplyfkhn
       61 nmaslirqln iygfrkvtnt dqglrsdree ieffhsffir gqecllefik rkvptsragg
      121 aatddgrdhs eafkgllsdv gsiqgrqehm dqsladmkre ngalwrevar lrqkhmkqqq
      181 iigklieflv tmvqanrnit vkrkvpfmlh dtsnpkmprl tkaafvtnyq vnspadslvs
      241 egpvihdvtd lmedlgetsg sivcepltag qspreievsr patpvqllas paatrdldtg
      301 pivasslnaa daieplvlld plpddqssyv apcdieevvv nssepspkep stasskslvv
      361 ptvktlqvat patsavkqtt pldsaftssn lfgdeeptll gdrhaangts dllsscvmle
      421 gipmfvgdsa asepasplgl tvpatshase gspkhkedsg aasvspdmqv alqdkafgnt
      481 kvfaehldsi dydldwlqdq lmtgalnldt stlmgvcpdw sgllsgslkl fssedpltsc
      541 lgdldtegrf ssdtignevv qytpslldlg ieedssfeav nelllsddev psslcttsia
      601 sgesisrpns lvasgkcsgl rqtsssvraa vqtvprsvtk kgtlsskkrr gsskk
//
LOCUS       XP_077503462             645 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113933 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503462
VERSION     XP_077503462.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647336.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..645
                     /product="uncharacterized protein LOC144113933 isoform X3"
                     /calculated_mol_wt=69654
     Region          14..112
                     /region_name="HSF_DNA-bind"
                     /note="HSF-type DNA-binding; pfam00447"
                     /db_xref="CDD:459813"
     Region          <489..>551
                     /region_name="Vert_HS_TF"
                     /note="Vertebrate heat shock transcription factor;
                     pfam06546"
                     /db_xref="CDD:461944"
     CDS             1..645
                     /gene="LOC144113933"
                     /coded_by="XM_077647336.1:153..2090"
                     /db_xref="GeneID:144113933"
ORIGIN      
        1 mhttefggss vpaflvklwk lvedeecndl iswnsngqsf iihnqtqfak ellplyfkhn
       61 nmaslirqln iygfrkvtnt dqglrsdree ieffhsffir gqecllefik rkvptsragg
      121 aatddgrdhs eafkgllsdv gsiqgrqehm dqsladmkre ngalwrevar lrqkhmkqqq
      181 iigklieflv tmvqanrnit vkrkvpfmlh dtsnpkmprl tkaafvtnyq vnspadslvs
      241 egpvihdvtd lmedlgetsg sivcepltag qspreievsr patpvqllas paatrdldtg
      301 pivasslnaa daieplvlld plpddqssyv apcdieevvv nssepspkep stasskslvv
      361 ptvktlqvat patsavkqtt pldsvsyeaf tssnlfgdee ptllgdrhaa ngtsdllssc
      421 vmlegipmfv gdsaasepas plgltvpats hasegspkhk edsgaasvsp dmqvalqdka
      481 fgntkvfaeh ldsidydldw lqdqlmtgal nldtstlmgl fssedpltsc lgdldtegrf
      541 ssdtignevv qytpslldlg ieedssfeav nelllsddev psslcttsia sgesisrpns
      601 lvasgkcsgl rqtsssvraa vqtvprsvtk kgtlsskkrr gsskk
//
LOCUS       XP_077503464             567 aa            linear   INV 25-NOV-2025
DEFINITION  heat shock factor protein-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077503464
VERSION     XP_077503464.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647338.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..567
                     /product="heat shock factor protein-like isoform X4"
                     /calculated_mol_wt=61410
     Region          14..112
                     /region_name="HSF_DNA-bind"
                     /note="HSF-type DNA-binding; pfam00447"
                     /db_xref="CDD:459813"
     CDS             1..567
                     /gene="LOC144113933"
                     /coded_by="XM_077647338.1:151..1854"
                     /db_xref="GeneID:144113933"
ORIGIN      
        1 mhttefggss vpaflvklwk lvedeecndl iswnsngqsf iihnqtqfak ellplyfkhn
       61 nmaslirqln iygfrkvtnt dqglrsdree ieffhsffir gqecllefik rkvptsragg
      121 aatddgrdhs eafkgllsdv gsiqgrqehm dqsladmkre ngalwrevar lrqkhmkqqq
      181 iigklieflv tmvqanrnit vkrkvpfmlh dtsnpkmprl tkaafvtnyq vnspadslvs
      241 egpvihdvtd lmedlgetsg sivcepltag qspreievsr patpvqllas paatrdldtg
      301 pivasslnaa daieplvlld plpddqssyv apcdieevvv nssepspkep stasskslvv
      361 ptvktlqvat patsavkqtt pldsvsyeaf tssnlfgdee ptllgdrhaa ngtsdllssc
      421 vmlegipmfv gdsaasepas plgltvpats hasegspkhk edsgaasvsp dmqvalqdka
      481 fgntkvfaeh ldsidydldw lqdqlmtgal nldtstlmgv cpdwsgllsg slklfssedp
      541 ltsclglgdr pqgncasrtn sqsnfls
//
LOCUS       XP_077503465             563 aa            linear   INV 25-NOV-2025
DEFINITION  heat shock factor protein-like isoform X5 [Amblyomma americanum].
ACCESSION   XP_077503465
VERSION     XP_077503465.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647339.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..563
                     /product="heat shock factor protein-like isoform X5"
                     /calculated_mol_wt=60932
     Region          14..112
                     /region_name="HSF_DNA-bind"
                     /note="HSF-type DNA-binding; pfam00447"
                     /db_xref="CDD:459813"
     CDS             1..563
                     /gene="LOC144113933"
                     /coded_by="XM_077647339.1:147..1838"
                     /db_xref="GeneID:144113933"
ORIGIN      
        1 mhttefggss vpaflvklwk lvedeecndl iswnsngqsf iihnqtqfak ellplyfkhn
       61 nmaslirqln iygfrkvtnt dqglrsdree ieffhsffir gqecllefik rkvptsragg
      121 aatddgrdhs eafkgllsdv gsiqgrqehm dqsladmkre ngalwrevar lrqkhmkqqq
      181 iigklieflv tmvqanrnit vkrkvpfmlh dtsnpkmprl tkaafvtnyq vnspadslvs
      241 egpvihdvtd lmedlgetsg sivcepltag qspreievsr patpvqllas paatrdldtg
      301 pivasslnaa daieplvlld plpddqssyv apcdieevvv nssepspkep stasskslvv
      361 ptvktlqvat patsavkqtt pldsaftssn lfgdeeptll gdrhaangts dllsscvmle
      421 gipmfvgdsa asepasplgl tvpatshase gspkhkedsg aasvspdmqv alqdkafgnt
      481 kvfaehldsi dydldwlqdq lmtgalnldt stlmgvcpdw sgllsgslkl fssedpltsc
      541 lglgdrpqgn casrtnsqsn fls
//
LOCUS       XP_077503466             553 aa            linear   INV 25-NOV-2025
DEFINITION  heat shock factor protein-like isoform X6 [Amblyomma americanum].
ACCESSION   XP_077503466
VERSION     XP_077503466.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647340.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..553
                     /product="heat shock factor protein-like isoform X6"
                     /calculated_mol_wt=59967
     Region          14..112
                     /region_name="HSF_DNA-bind"
                     /note="HSF-type DNA-binding; pfam00447"
                     /db_xref="CDD:459813"
     Region          <436..>523
                     /region_name="Vert_HS_TF"
                     /note="Vertebrate heat shock transcription factor;
                     pfam06546"
                     /db_xref="CDD:461944"
     CDS             1..553
                     /gene="LOC144113933"
                     /coded_by="XM_077647340.1:151..1812"
                     /db_xref="GeneID:144113933"
ORIGIN      
        1 mhttefggss vpaflvklwk lvedeecndl iswnsngqsf iihnqtqfak ellplyfkhn
       61 nmaslirqln iygfrkvtnt dqglrsdree ieffhsffir gqecllefik rkvptsragg
      121 aatddgrdhs eafkgllsdv gsiqgrqehm dqsladmkre ngalwrevar lrqkhmkqqq
      181 iigklieflv tmvqanrnit vkrkvpfmlh dtsnpkmprl tkaafvtnyq vnspadslvs
      241 egpvihdvtd lmedlgetsg sivcepltag qspreievsr patpvqllas paatrdldtg
      301 pivasslnaa daieplvlld plpddqssyv apcdieevvv nssepspkep stasskslvv
      361 ptvktlqvat patsavkqtt pldsvsyeaf tssnlfgdee ptllgdrhaa ngtsdllssc
      421 vmlegipmfv gdsaasepas plgltvpats hasegspkhk edsgaasvsp dmqvalqdka
      481 fgntkvfaeh ldsidydldw lqdqlmtgal nldtstlmgl fssedpltsc lglgdrpqgn
      541 casrtnsqsn fls
//
LOCUS       XP_077503467            1147 aa            linear   INV 25-NOV-2025
DEFINITION  strumpellin and WASH-interacting protein [Amblyomma americanum].
ACCESSION   XP_077503467
VERSION     XP_077503467.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647341.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1147
                     /product="strumpellin and WASH-interacting protein"
                     /calculated_mol_wt=132450
     Region          27..590
                     /region_name="WASH-4_N"
                     /note="WASH complex subunit 4, N-terminal; pfam14745"
                     /db_xref="CDD:434178"
     Region          596..939
                     /region_name="WASH-7_mid"
                     /note="WASH complex subunit 7; pfam14744"
                     /db_xref="CDD:434177"
     Region          957..1126
                     /region_name="WASH-7_C"
                     /note="WASH complex subunit 7, C-terminal; pfam14746"
                     /db_xref="CDD:464293"
     CDS             1..1147
                     /gene="SWIP"
                     /coded_by="XM_077647341.1:378..3821"
                     /db_xref="GeneID:144113935"
ORIGIN      
        1 maserwdkdi degvvrivgq lqlrkygkfm edlttefkni eqslddgite awdvdsdlia
       61 lhwvpyentt llelistdnk vlnkvmtvla alcaemkqlc heakskfhea ilfygegsls
      121 sntvpdgesq vavgrmigim qdlschvsrc ylvaknviqq msalydrnka acvidvtdvh
      181 fqtvfehlge mlaslvtlde ivqaqgglre hwtlyrrmlk svhnncakfn vvpedmkald
      241 klmgeleykl lqgrifmgcv eqgfddntlf vaknaafaee fahnirswfa qvdaklgetn
      301 eldkrkqlig iyalyvlyyy iyrnvdkkff ktlwdvykkv pavhvlgnil wfpdqflllq
      361 mpqvmrsldk kaqdavkaqr lsylqqksav lsrdvqalhv qvsswlvrme svfqkdtdkl
      421 ledlnrrssl llqgiyltwy ishqvktlmn lhvalnkpmt kssvlqlckm iemmkaiegm
      481 fhqqtlkicd svthivqhls yvalfavsna kkrlvsdkky serrldvlsa lvlvekclsg
      541 patkerrlii hlaiavgvqm kcfkdeeias fysvmrkldm igelheklqe gsdgsflywh
      601 rvvfpifldd vyesavdghr ihylvaalkd sarpikttkh dsdpqaildi freeiygqfk
      661 ehildklckd vetdlrlqth chlqlddrnp frvglkdyaq ilhirpirll gqvinvrayv
      721 ehyldktfyn lttvalhdwk tygemrsmar qkyglstved hlpsqtleqg ldvleimrni
      781 hifvsryyyn lnnqifvert snnkhlntin irhiansirt hgigimnttv nftyqflrkk
      841 fyifsqflyd ehiksrlikd lrhfrelrsq sdpkypfera ekfnrgirkl gltpdgesfl
      901 dqfrtlvsqv gnamgyvrmi rsgglhccsn aiqfvpdley vanfeglsre allgdactea
      961 ahrldavvts laknfsegse yfrllvdvfs ptfrdpsnmh lrnffvilpp ltlnyvehav
     1021 sckermsrkn kvgaaftddg famgvayilk lldqyqdfds lhwfqsvsek fqkdtaqvre
     1081 qkkeaagked eklqqamslt gkrldtyrhe fdllnyslss ariffradlt aeeekqekad
     1141 ditktph
//
LOCUS       XP_077503468             625 aa            linear   INV 25-NOV-2025
DEFINITION  rab geranylgeranyltransferase subunit alpha isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503468
VERSION     XP_077503468.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647342.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..625
                     /product="rab geranylgeranyltransferase subunit alpha
                     isoform X1"
                     /calculated_mol_wt=70606
     Region          93..>309
                     /region_name="BET4"
                     /note="Protein prenyltransferase, alpha subunit
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG5536"
                     /db_xref="CDD:227823"
     Region          <524..>605
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          524..545
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          546..567
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          568..592
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          593..616
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..625
                     /gene="RabGGTa"
                     /coded_by="XM_077647342.1:73..1950"
                     /db_xref="GeneID:144113936"
ORIGIN      
        1 mlifssntst hlllraslgc svvfgssets ssvldgpwlt tapaprvcpl aagspgkrrk
       61 rhseniimhg rvkvkttaek eeekrkerek kleiykaltq qviekrqkge fddellrvtg
      121 qilqsnpdyt tmwnirrevf mvhfeksckk taedgagell lteaalqknp ksygawshra
      181 wameafpgmd wdkelrlcnl fldqdernfh cwdyrrfvcg htevtgemel aftmdriaan
      241 fsnysawhyr ssllpniypg prqgtvqedv lleeyslvqn atftdpgdqs awfyhrwltg
      301 rekpaldfll fyvsretcqv ivhftrqisl sdteitvtmn gsllplswka pcqslcsslw
      361 yaslsegslp gdctltamvk tmnnecasad lsltraqqes kasgniprnh lfscelsaar
      421 tcvlenelkt crelhdleph nkwplltcvl lmraldgsrf rmdikrflae ltavdpmrrn
      481 yyndldskfa aesvieqlne ndmaadfsgl gltsichtnh lallrevdis knqiksllpl
      541 gcllsirkit lddncvetcd glgsllklat lslrnnkied kdclfvlktc psltelnleg
      601 npvseskdyv hsllpqvkll dgsrl
//
LOCUS       XP_077503469             623 aa            linear   INV 25-NOV-2025
DEFINITION  rab geranylgeranyltransferase subunit alpha isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503469
VERSION     XP_077503469.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647343.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..623
                     /product="rab geranylgeranyltransferase subunit alpha
                     isoform X2"
                     /calculated_mol_wt=70322
     Region          93..>307
                     /region_name="BET4"
                     /note="Protein prenyltransferase, alpha subunit
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG5536"
                     /db_xref="CDD:227823"
     Region          <522..>603
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          522..543
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          544..565
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          566..590
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          591..614
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..623
                     /gene="RabGGTa"
                     /coded_by="XM_077647343.1:75..1946"
                     /db_xref="GeneID:144113936"
ORIGIN      
        1 mlifssntst hlllraslgc svvfgssets ssvldgpwlt tapaprvcpl aagspgkrrk
       61 rhseniimhg rvkvkttaek eeekrkerek kleiykaltq qviekkgefd dellrvtgqi
      121 lqsnpdyttm wnirrevfmv hfeksckkta edgagelllt eaalqknpks ygawshrawa
      181 meafpgmdwd kelrlcnlfl dqdernfhcw dyrrfvcght evtgemelaf tmdriaanfs
      241 nysawhyrss llpniypgpr qgtvqedvll eeyslvqnat ftdpgdqsaw fyhrwltgre
      301 kpaldfllfy vsretcqviv hftrqislsd teitvtmngs llplswkapc qslcsslwya
      361 slsegslpgd ctltamvktm nnecasadls ltraqqeska sgniprnhlf scelsaartc
      421 vlenelktcr elhdlephnk wplltcvllm raldgsrfrm dikrflaelt avdpmrrnyy
      481 ndldskfaae svieqlnend maadfsglgl tsichtnhla llrevdiskn qiksllplgc
      541 llsirkitld dncvetcdgl gsllklatls lrnnkiedkd clfvlktcps ltelnlegnp
      601 vseskdyvhs llpqvklldg srl
//
LOCUS       XP_077503470             558 aa            linear   INV 25-NOV-2025
DEFINITION  rab geranylgeranyltransferase subunit alpha isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503470
VERSION     XP_077503470.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647344.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..558
                     /product="rab geranylgeranyltransferase subunit alpha
                     isoform X3"
                     /calculated_mol_wt=63557
     Region          26..>242
                     /region_name="BET4"
                     /note="Protein prenyltransferase, alpha subunit
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG5536"
                     /db_xref="CDD:227823"
     Region          <457..>538
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          457..478
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          479..500
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          501..525
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          526..549
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..558
                     /gene="RabGGTa"
                     /coded_by="XM_077647344.1:248..1924"
                     /db_xref="GeneID:144113936"
ORIGIN      
        1 mhgrvkvktt aekeeekrke rekkleiyka ltqqviekrq kgefddellr vtgqilqsnp
       61 dyttmwnirr evfmvhfeks ckktaedgag elllteaalq knpksygaws hrawameafp
      121 gmdwdkelrl cnlfldqder nfhcwdyrrf vcghtevtge melaftmdri aanfsnysaw
      181 hyrssllpni ypgprqgtvq edvlleeysl vqnatftdpg dqsawfyhrw ltgrekpald
      241 fllfyvsret cqvivhftrq islsdteitv tmngsllpls wkapcqslcs slwyaslseg
      301 slpgdctlta mvktmnneca sadlsltraq qeskasgnip rnhlfscels aartcvlene
      361 lktcrelhdl ephnkwpllt cvllmraldg srfrmdikrf laeltavdpm rrnyyndlds
      421 kfaaesvieq lnendmaadf sglgltsich tnhlallrev disknqiksl lplgcllsir
      481 kitlddncve tcdglgsllk latlslrnnk iedkdclfvl ktcpslteln legnpvsesk
      541 dyvhsllpqv klldgsrl
//
LOCUS       XP_077503471             558 aa            linear   INV 25-NOV-2025
DEFINITION  rab geranylgeranyltransferase subunit alpha isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503471
VERSION     XP_077503471.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647345.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..558
                     /product="rab geranylgeranyltransferase subunit alpha
                     isoform X3"
                     /calculated_mol_wt=63557
     Region          26..>242
                     /region_name="BET4"
                     /note="Protein prenyltransferase, alpha subunit
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG5536"
                     /db_xref="CDD:227823"
     Region          <457..>538
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          457..478
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          479..500
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          501..525
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          526..549
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..558
                     /gene="RabGGTa"
                     /coded_by="XM_077647345.1:148..1824"
                     /db_xref="GeneID:144113936"
ORIGIN      
        1 mhgrvkvktt aekeeekrke rekkleiyka ltqqviekrq kgefddellr vtgqilqsnp
       61 dyttmwnirr evfmvhfeks ckktaedgag elllteaalq knpksygaws hrawameafp
      121 gmdwdkelrl cnlfldqder nfhcwdyrrf vcghtevtge melaftmdri aanfsnysaw
      181 hyrssllpni ypgprqgtvq edvlleeysl vqnatftdpg dqsawfyhrw ltgrekpald
      241 fllfyvsret cqvivhftrq islsdteitv tmngsllpls wkapcqslcs slwyaslseg
      301 slpgdctlta mvktmnneca sadlsltraq qeskasgnip rnhlfscels aartcvlene
      361 lktcrelhdl ephnkwpllt cvllmraldg srfrmdikrf laeltavdpm rrnyyndlds
      421 kfaaesvieq lnendmaadf sglgltsich tnhlallrev disknqiksl lplgcllsir
      481 kitlddncve tcdglgsllk latlslrnnk iedkdclfvl ktcpslteln legnpvsesk
      541 dyvhsllpqv klldgsrl
//
LOCUS       XP_077503472             558 aa            linear   INV 25-NOV-2025
DEFINITION  rab geranylgeranyltransferase subunit alpha isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503472
VERSION     XP_077503472.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647346.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..558
                     /product="rab geranylgeranyltransferase subunit alpha
                     isoform X4"
                     /calculated_mol_wt=63557
     Region          26..>242
                     /region_name="BET4"
                     /note="Protein prenyltransferase, alpha subunit
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG5536"
                     /db_xref="CDD:227823"
     Region          <457..>538
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          457..478
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          479..500
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          501..525
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          526..549
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..558
                     /gene="RabGGTa"
                     /coded_by="XM_077647346.1:130..1806"
                     /db_xref="GeneID:144113936"
ORIGIN      
        1 mhgrvkvktt aekeeekrke rekkleiyka ltqqviekrq kgefddellr vtgqilqsnp
       61 dyttmwnirr evfmvhfeks ckktaedgag elllteaalq knpksygaws hrawameafp
      121 gmdwdkelrl cnlfldqder nfhcwdyrrf vcghtevtge melaftmdri aanfsnysaw
      181 hyrssllpni ypgprqgtvq edvlleeysl vqnatftdpg dqsawfyhrw ltgrekpald
      241 fllfyvsret cqvivhftrq islsdteitv tmngsllpls wkapcqslcs slwyaslseg
      301 slpgdctlta mvktmnneca sadlsltraq qeskasgnip rnhlfscels aartcvlene
      361 lktcrelhdl ephnkwpllt cvllmraldg srfrmdikrf laeltavdpm rrnyyndlds
      421 kfaaesvieq lnendmaadf sglgltsich tnhlallrev disknqiksl lplgcllsir
      481 kitlddncve tcdglgsllk latlslrnnk iedkdclfvl ktcpslteln legnpvsesk
      541 dyvhsllpqv klldgsrl
//
LOCUS       XP_077503473             263 aa            linear   INV 25-NOV-2025
DEFINITION  THAP domain-containing protein 11-like [Amblyomma americanum].
ACCESSION   XP_077503473
VERSION     XP_077503473.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647347.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..263
                     /product="THAP domain-containing protein 11-like"
                     /calculated_mol_wt=28823
     Region          14..95
                     /region_name="THAP"
                     /note="The THAP domain is a putative DNA-binding domain
                     (DBD) and probably also binds a zinc ion; smart00980"
                     /db_xref="CDD:214951"
     CDS             1..263
                     /gene="LOC144113938"
                     /coded_by="XM_077647347.1:93..884"
                     /db_xref="GeneID:144113938"
ORIGIN      
        1 mdvgrarkat nrryccvkdc hsregngdvk lfrfparpye strrlkwiva vrlvngedfs
       61 nwspndntri cskhfvngek stiethpgyl ptifpsvhrk rsrdsagqls rfnrwmqrsa
      121 vratacpqer eavacatddg hsptfgpaeq dtsggepwng ldflcaaaei hsavasvetq
      181 tdhesagsgs fsvficcsqg cdvstqithr etrdcgvqhk pavtstvsgp dhrtsyfagy
      241 dsiekpsgal edlcsvncvv fal
//
LOCUS       XP_077503474             521 aa            linear   INV 25-NOV-2025
DEFINITION  L-type amino acid transporter sobremesa [Amblyomma americanum].
ACCESSION   XP_077503474
VERSION     XP_077503474.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647348.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..521
                     /product="L-type amino acid transporter sobremesa"
                     /calculated_mol_wt=55588
     Region          38..511
                     /region_name="2A0308"
                     /note="L-type amino acid transporter; TIGR00911"
                     /db_xref="CDD:273332"
     CDS             1..521
                     /gene="sbm"
                     /coded_by="XM_077647348.1:132..1697"
                     /db_xref="GeneID:144113939"
ORIGIN      
        1 msagydyapv yrpttapldq anghvaanga vpeddvvsvt sqqgavqlkr rvgllsgval
       61 ivgtmigsgi fvspkgvler sgsvglsliv wagcgvlslf galslaelgt mihksggdfi
      121 yiltafagdp pargglkpvp aflhawttvl llkpaglgim alsfakyivq pafidcgdsp
      181 pvptkmvaag ciclvtfvnc fsvklatriq niftvtklva vaviiiagvv miakghtqyl
      241 stgfqgttss isdiatafys glwaydgwnn lnyiteelin pyvnlprsim igiplvtlcy
      301 vlinvsymsv msatellase avavrfgnhv lgpaavlisl fvaastfgsg ngttfttari
      361 gfvaareghl sevlsyahar rltpvpalal ncalsicmvs ladigslidf fsfaawmfyg
      421 atmlaliilr wtkkdaprpy kvpivipwiv lllsiylvaa piiqnpqvey vyaclfivsg
      481 llfyvpfvhy rlrlgimrkf tilvqlllnv vpttykntvq n
//
LOCUS       XP_077503475             452 aa            linear   INV 25-NOV-2025
DEFINITION  casein kinase II subunit alpha isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503475
VERSION     XP_077503475.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647349.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..452
                     /product="casein kinase II subunit alpha isoform X1"
                     /calculated_mol_wt=51831
     Region          89..394
                     /region_name="STKc_CK2_alpha"
                     /note="Catalytic subunit (alpha) of the Serine/Threonine
                     Kinase, Casein Kinase 2; cd14132"
                     /db_xref="CDD:271034"
     Site            order(105,108..110,136,138,170,172..173,179)
                     /site_type="other"
                     /note="allosteric inhibitor binding site [chemical
                     binding]"
                     /db_xref="CDD:271034"
     Site            order(105..106,108..111,119,123,172)
                     /site_type="other"
                     /note="tetramer interface [polypeptide binding]"
                     /db_xref="CDD:271034"
     Site            order(105..106,108..110,126,128,172)
                     /site_type="other"
                     /note="CK2 beta interface [polypeptide binding]"
                     /db_xref="CDD:271034"
     Site            order(114..118,122,135,137,149,164,182..185,189,191..192,
                     225,227,229..230,232,244,247,258,260..263,265,304)
                     /site_type="active"
                     /db_xref="CDD:271034"
     Site            order(116..117,119..120,122,135,137,164,182..183,185,
                     229..230,232,243..244)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271034"
     Site            order(149,191,225,227,247,258,260..263,265,304)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271034"
     Site            order(243..253,258..265)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271034"
     CDS             1..452
                     /gene="CkIIalpha"
                     /coded_by="XM_077647349.1:325..1683"
                     /db_xref="GeneID:144113940"
ORIGIN      
        1 mvrvtlvlal slsvylcael afyasqvssr cpgtlsrasl laltsvwggk gglcdrsgpr
       61 gkqfgssrgr qmplpsrsrv ypdvnshrpr dywdyeshvv ewgvqddyql vrklgrgkys
      121 evfeainvtn nekcvvkilk pvkkkkikre ikilenlrgg pnivtlqavv kdlvsrtpal
      181 ifehvnntdf kqlyqtltdy diryylyell kaldyshsmg imhrdvkphn vmidhecrrl
      241 rlidwglaef yhpgqeynvr vasryfkgpe llvdyqmydy sldmwslgcm lasmifrrep
      301 ffhghdnydq lvriakvlgt eelfeyleky qaeldprfnd ilgrhsrkrw erfvhsenqh
      361 lvtpealdfl dkllrydhqe rltareameh pyfypvskeq qqkfmdrrta hsgspppvsa
      421 ggapvgsnal gvlptspvts psvaalgstt st
//
LOCUS       XP_077503476             381 aa            linear   INV 25-NOV-2025
DEFINITION  casein kinase II subunit alpha isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503476
VERSION     XP_077503476.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647350.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..381
                     /product="casein kinase II subunit alpha isoform X2"
                     /calculated_mol_wt=44371
     Region          18..323
                     /region_name="STKc_CK2_alpha"
                     /note="Catalytic subunit (alpha) of the Serine/Threonine
                     Kinase, Casein Kinase 2; cd14132"
                     /db_xref="CDD:271034"
     Site            order(34,37..39,65,67,99,101..102,108)
                     /site_type="other"
                     /note="allosteric inhibitor binding site [chemical
                     binding]"
                     /db_xref="CDD:271034"
     Site            order(34..35,37..40,48,52,101)
                     /site_type="other"
                     /note="tetramer interface [polypeptide binding]"
                     /db_xref="CDD:271034"
     Site            order(34..35,37..39,55,57,101)
                     /site_type="other"
                     /note="CK2 beta interface [polypeptide binding]"
                     /db_xref="CDD:271034"
     Site            order(43..47,51,64,66,78,93,111..114,118,120..121,154,156,
                     158..159,161,173,176,187,189..192,194,233)
                     /site_type="active"
                     /db_xref="CDD:271034"
     Site            order(45..46,48..49,51,64,66,93,111..112,114,158..159,161,
                     172..173)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271034"
     Site            order(78,120,154,156,176,187,189..192,194,233)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271034"
     Site            order(172..182,187..194)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271034"
     CDS             1..381
                     /gene="CkIIalpha"
                     /coded_by="XM_077647350.1:296..1441"
                     /db_xref="GeneID:144113940"
ORIGIN      
        1 mplpsrsrvy pdvnshrprd ywdyeshvve wgvqddyqlv rklgrgkyse vfeainvtnn
       61 ekcvvkilkp vkkkkikrei kilenlrggp nivtlqavvk dlvsrtpali fehvnntdfk
      121 qlyqtltdyd iryylyellk aldyshsmgi mhrdvkphnv midhecrrlr lidwglaefy
      181 hpgqeynvrv asryfkgpel lvdyqmydys ldmwslgcml asmifrrepf fhghdnydql
      241 vriakvlgte elfeylekyq aeldprfndi lgrhsrkrwe rfvhsenqhl vtpealdfld
      301 kllrydhqer ltareamehp yfypvskeqq qkfmdrrtah sgspppvsag gapvgsnalg
      361 vlptspvtsp svaalgstts t
//
LOCUS       XP_077503477             381 aa            linear   INV 25-NOV-2025
DEFINITION  casein kinase II subunit alpha isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503477
VERSION     XP_077503477.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647351.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..381
                     /product="casein kinase II subunit alpha isoform X2"
                     /calculated_mol_wt=44371
     Region          18..323
                     /region_name="STKc_CK2_alpha"
                     /note="Catalytic subunit (alpha) of the Serine/Threonine
                     Kinase, Casein Kinase 2; cd14132"
                     /db_xref="CDD:271034"
     Site            order(34,37..39,65,67,99,101..102,108)
                     /site_type="other"
                     /note="allosteric inhibitor binding site [chemical
                     binding]"
                     /db_xref="CDD:271034"
     Site            order(34..35,37..40,48,52,101)
                     /site_type="other"
                     /note="tetramer interface [polypeptide binding]"
                     /db_xref="CDD:271034"
     Site            order(34..35,37..39,55,57,101)
                     /site_type="other"
                     /note="CK2 beta interface [polypeptide binding]"
                     /db_xref="CDD:271034"
     Site            order(43..47,51,64,66,78,93,111..114,118,120..121,154,156,
                     158..159,161,173,176,187,189..192,194,233)
                     /site_type="active"
                     /db_xref="CDD:271034"
     Site            order(45..46,48..49,51,64,66,93,111..112,114,158..159,161,
                     172..173)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271034"
     Site            order(78,120,154,156,176,187,189..192,194,233)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271034"
     Site            order(172..182,187..194)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271034"
     CDS             1..381
                     /gene="CkIIalpha"
                     /coded_by="XM_077647351.1:593..1738"
                     /db_xref="GeneID:144113940"
ORIGIN      
        1 mplpsrsrvy pdvnshrprd ywdyeshvve wgvqddyqlv rklgrgkyse vfeainvtnn
       61 ekcvvkilkp vkkkkikrei kilenlrggp nivtlqavvk dlvsrtpali fehvnntdfk
      121 qlyqtltdyd iryylyellk aldyshsmgi mhrdvkphnv midhecrrlr lidwglaefy
      181 hpgqeynvrv asryfkgpel lvdyqmydys ldmwslgcml asmifrrepf fhghdnydql
      241 vriakvlgte elfeylekyq aeldprfndi lgrhsrkrwe rfvhsenqhl vtpealdfld
      301 kllrydhqer ltareamehp yfypvskeqq qkfmdrrtah sgspppvsag gapvgsnalg
      361 vlptspvtsp svaalgstts t
//
LOCUS       XP_077503478             381 aa            linear   INV 25-NOV-2025
DEFINITION  casein kinase II subunit alpha isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503478
VERSION     XP_077503478.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647352.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..381
                     /product="casein kinase II subunit alpha isoform X2"
                     /calculated_mol_wt=44371
     Region          18..323
                     /region_name="STKc_CK2_alpha"
                     /note="Catalytic subunit (alpha) of the Serine/Threonine
                     Kinase, Casein Kinase 2; cd14132"
                     /db_xref="CDD:271034"
     Site            order(34,37..39,65,67,99,101..102,108)
                     /site_type="other"
                     /note="allosteric inhibitor binding site [chemical
                     binding]"
                     /db_xref="CDD:271034"
     Site            order(34..35,37..40,48,52,101)
                     /site_type="other"
                     /note="tetramer interface [polypeptide binding]"
                     /db_xref="CDD:271034"
     Site            order(34..35,37..39,55,57,101)
                     /site_type="other"
                     /note="CK2 beta interface [polypeptide binding]"
                     /db_xref="CDD:271034"
     Site            order(43..47,51,64,66,78,93,111..114,118,120..121,154,156,
                     158..159,161,173,176,187,189..192,194,233)
                     /site_type="active"
                     /db_xref="CDD:271034"
     Site            order(45..46,48..49,51,64,66,93,111..112,114,158..159,161,
                     172..173)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271034"
     Site            order(78,120,154,156,176,187,189..192,194,233)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271034"
     Site            order(172..182,187..194)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271034"
     CDS             1..381
                     /gene="CkIIalpha"
                     /coded_by="XM_077647352.1:414..1559"
                     /db_xref="GeneID:144113940"
ORIGIN      
        1 mplpsrsrvy pdvnshrprd ywdyeshvve wgvqddyqlv rklgrgkyse vfeainvtnn
       61 ekcvvkilkp vkkkkikrei kilenlrggp nivtlqavvk dlvsrtpali fehvnntdfk
      121 qlyqtltdyd iryylyellk aldyshsmgi mhrdvkphnv midhecrrlr lidwglaefy
      181 hpgqeynvrv asryfkgpel lvdyqmydys ldmwslgcml asmifrrepf fhghdnydql
      241 vriakvlgte elfeylekyq aeldprfndi lgrhsrkrwe rfvhsenqhl vtpealdfld
      301 kllrydhqer ltareamehp yfypvskeqq qkfmdrrtah sgspppvsag gapvgsnalg
      361 vlptspvtsp svaalgstts t
//
LOCUS       XP_077503479             947 aa            linear   INV 25-NOV-2025
DEFINITION  DNA topoisomerase I, mitochondrial-like [Amblyomma americanum].
ACCESSION   XP_077503479
VERSION     XP_077503479.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647353.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..947
                     /product="DNA topoisomerase I, mitochondrial-like"
                     /calculated_mol_wt=108523
     Region          397..611
                     /region_name="Topoisom_I_N"
                     /note="Eukaryotic DNA topoisomerase I, DNA binding
                     fragment; pfam02919"
                     /db_xref="CDD:460746"
     Region          542..919
                     /region_name="TOPEUc"
                     /note="DNA Topoisomerase I (eukaryota); smart00435"
                     /db_xref="CDD:214661"
     CDS             1..947
                     /gene="LOC144113941"
                     /coded_by="XM_077647353.1:171..3014"
                     /db_xref="GeneID:144113941"
ORIGIN      
        1 memlemnsae gssptglksp sgshgdcgnv shgltngvng vvvnnttsss sshhksssdk
       61 hnhssskhhk sskehrddhh khkdkhkdkh kdksstgttg ssskhkeghh sssskghsss
      121 kehksssssk pkdvisspss ahkttevkke sssskeskps seskhkdtss kdkdkdrhkd
      181 khkehkdkdk hrdkdkdrek hksssekhrd ssspvkvkqe vgtakkpsqe glspppakvk
      241 velsspphpp aprmtletet vdiseikgev lsdddvplsa rkpqqrlset sppkgkhame
      301 tdseedepls vrsqkaakki kseekakkkr kaddsdyedn ikhakkaaap kpkkartevk
      361 sepkaeegsp kkkgkvkkee evevwkwwee ekqndgkkwh tlehrgpvfa ppyeplpdnv
      421 rfyydgvpvk lspeaeevag fygrmldhdy tskdafnknf fhdwrkcmta keaelitdlk
      481 kcnfkeidty yklkseerka mskeekqaik vqneklveey gfciidghkq kignfkiepp
      541 glfrgrgehp kmgmlkrrim pedviinigk eakvpdppag hkwkevrhdn svswlsswte
      601 nilgqtkyim lnpssklkge kdmqkyemar klkscvdkir qnymndwksk emrirqrsva
      661 lyfidklalr agnekeeget adtvgccslr vehiklhdek dgkefvvefd flgkdsiryv
      721 navpvekrvy knlklfmenk kpqddlfdrl ntsilnkhln elmegltakv frtynasktl
      781 qeqlelltea dmslpekmla ynranravai lcnhqravpk tfgkqmenlq kkmeekeeqi
      841 rqckvelkql ksdynnsrte kdkqllerkk krmqqleeqm kklevqamdk eenkqialgt
      901 sklnyldpri svawckkwdv pidkiynktq rdkfrwaiem agpdyqf
//
LOCUS       XP_077503480             269 aa            linear   INV 25-NOV-2025
DEFINITION  protein FAM210A [Amblyomma americanum].
ACCESSION   XP_077503480
VERSION     XP_077503480.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647354.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..269
                     /product="protein FAM210A"
                     /calculated_mol_wt=30418
     Region          151..238
                     /region_name="FAM210A-B_dom"
                     /note="FAM210A/B-like domain; pfam06916"
                     /db_xref="CDD:429191"
     CDS             1..269
                     /gene="LOC144113942"
                     /coded_by="XM_077647354.1:46..855"
                     /db_xref="GeneID:144113942"
ORIGIN      
        1 mprsafstcl lnsclcgias rtwwsrwlsr difiavvias msatfswlwr advsscrlpr
       61 llylrhiplq ktnrllpylr whkayirhsp lescaaaarp sfiscthsfs seyctksdar
      121 agfkkdvtsp leasaepkke evmeeklsif qrykkmfkey wyvmlpvhia tsivwfgsff
      181 ylatcgvdvv pileflglpe svisplrksg lnyiatasam yklatparyt vtlggtsiai
      241 ralvkrglik pmptggelrq mlrdkikpq
//
LOCUS       XP_077503481             526 aa            linear   INV 25-NOV-2025
DEFINITION  N-acetylgalactosamine-6-sulfatase-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503481
VERSION     XP_077503481.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647355.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..526
                     /product="N-acetylgalactosamine-6-sulfatase-like isoform
                     X1"
                     /calculated_mol_wt=58025
     Region          26..498
                     /region_name="ALP_like"
                     /note="alkaline phosphatases and sulfatases; cl23718"
                     /db_xref="CDD:474031"
     Site            order(34,74,232,284..285)
                     /site_type="active"
                     /db_xref="CDD:293732"
     Site            order(34,74,232,285)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:293732"
     CDS             1..526
                     /gene="LOC144113944"
                     /coded_by="XM_077647355.1:202..1782"
                     /db_xref="GeneID:144113944"
ORIGIN      
        1 mnavravrmg lvvlvtaqws gaaaspnfvl mvmddmgwgd lgvlgqpake tphldrmase
       61 gmlltdfyaa spvcspsraa lltgrlpirs gfytsnnhsr nayvspsvvg gvpdeellip
      121 eilgevgyrn mivgkwhlgh rppflplhhg fheffgspst hpgpfddvtq pnipvfrddh
      181 migryyedfk idvktatsnv tviyteeavs firrqaaqrq sfflywapds thtpvyasev
      241 vrgrsvrgay gdavieldrg vgtildtlrs tgiaentlvf ftsdngaaty eresgsypre
      301 menggsngpl lcgklttfeg gvrepaiaww pgtipagtvs rlpvsnmdll ptiaelagat
      361 lppglvldgh slvhgvllgr rnnppterpv fyyrgnelma vrsglykahf wcwtdfkgdl
      421 nvcpgqhvdg vtshkqlnyt dqpvlfhlgr dpgekfpipp hlheyrehit pilaiyrqhk
      481 rsvvpgkpql dwcdaaamqw appgcekidr clpvppsrpq rcswir
//
LOCUS       XP_077503482             526 aa            linear   INV 25-NOV-2025
DEFINITION  N-acetylgalactosamine-6-sulfatase-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503482
VERSION     XP_077503482.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647356.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..526
                     /product="N-acetylgalactosamine-6-sulfatase-like isoform
                     X1"
                     /calculated_mol_wt=58025
     Region          26..498
                     /region_name="ALP_like"
                     /note="alkaline phosphatases and sulfatases; cl23718"
                     /db_xref="CDD:474031"
     Site            order(34,74,232,284..285)
                     /site_type="active"
                     /db_xref="CDD:293732"
     Site            order(34,74,232,285)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:293732"
     CDS             1..526
                     /gene="LOC144113944"
                     /coded_by="XM_077647356.1:197..1777"
                     /db_xref="GeneID:144113944"
ORIGIN      
        1 mnavravrmg lvvlvtaqws gaaaspnfvl mvmddmgwgd lgvlgqpake tphldrmase
       61 gmlltdfyaa spvcspsraa lltgrlpirs gfytsnnhsr nayvspsvvg gvpdeellip
      121 eilgevgyrn mivgkwhlgh rppflplhhg fheffgspst hpgpfddvtq pnipvfrddh
      181 migryyedfk idvktatsnv tviyteeavs firrqaaqrq sfflywapds thtpvyasev
      241 vrgrsvrgay gdavieldrg vgtildtlrs tgiaentlvf ftsdngaaty eresgsypre
      301 menggsngpl lcgklttfeg gvrepaiaww pgtipagtvs rlpvsnmdll ptiaelagat
      361 lppglvldgh slvhgvllgr rnnppterpv fyyrgnelma vrsglykahf wcwtdfkgdl
      421 nvcpgqhvdg vtshkqlnyt dqpvlfhlgr dpgekfpipp hlheyrehit pilaiyrqhk
      481 rsvvpgkpql dwcdaaamqw appgcekidr clpvppsrpq rcswir
//
LOCUS       XP_077503483             517 aa            linear   INV 25-NOV-2025
DEFINITION  N-acetylgalactosamine-6-sulfatase-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503483
VERSION     XP_077503483.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647357.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..517
                     /product="N-acetylgalactosamine-6-sulfatase-like isoform
                     X2"
                     /calculated_mol_wt=56961
     Region          26..489
                     /region_name="ALP_like"
                     /note="alkaline phosphatases and sulfatases; cl23718"
                     /db_xref="CDD:474031"
     Site            order(34,74,232,284..285)
                     /site_type="active"
                     /db_xref="CDD:293732"
     Site            order(34,74,232,285)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:293732"
     CDS             1..517
                     /gene="LOC144113944"
                     /coded_by="XM_077647357.1:202..1755"
                     /db_xref="GeneID:144113944"
ORIGIN      
        1 mnavravrmg lvvlvtaqws gaaaspnfvl mvmddmgwgd lgvlgqpake tphldrmase
       61 gmlltdfyaa spvcspsraa lltgrlpirs gfytsnnhsr nayvspsvvg gvpdeellip
      121 eilgevgyrn mivgkwhlgh rppflplhhg fheffgspst hpgpfddvtq pnipvfrddh
      181 migryyedfk idvktatsnv tviyteeavs firrqaaqrq sfflywapds thtpvyasev
      241 vrgrsvrgay gdavieldrg vgtildtlrs tgiaentlvf ftsdngaaty eresggsngp
      301 llcgklttfe ggvrepaiaw wpgtipagtv srlpvsnmdl lptiaelaga tlppglvldg
      361 hslvhgvllg rrnnppterp vfyyrgnelm avrsglykah fwcwtdfkgd lnvcpgqhvd
      421 gvtshkqlny tdqpvlfhlg rdpgekfpip phlheyrehi tpilaiyrqh krsvvpgkpq
      481 ldwcdaaamq wappgcekid rclpvppsrp qrcswir
//
LOCUS       XP_077503484             537 aa            linear   INV 25-NOV-2025
DEFINITION  N-acetylgalactosamine-6-sulfatase-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503484
VERSION     XP_077503484.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647358.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..537
                     /product="N-acetylgalactosamine-6-sulfatase-like isoform
                     X1"
                     /calculated_mol_wt=59949
     Region          37..509
                     /region_name="ALP_like"
                     /note="alkaline phosphatases and sulfatases; cl23718"
                     /db_xref="CDD:474031"
     Site            order(46,86,244,296..297)
                     /site_type="active"
                     /db_xref="CDD:293732"
     Site            order(46,86,244,297)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:293732"
     CDS             1..537
                     /gene="LOC144113945"
                     /coded_by="XM_077647358.1:113..1726"
                     /db_xref="GeneID:144113945"
ORIGIN      
        1 mwhhrleats iamlvhvcvc amlsttlaln ytdaspkpnf vimlmddmgw gdlgifgnpa
       61 ketpnldrma aegalltdfy tanplcspsr aalltgrlpi rngfyttnnh srngytpqei
      121 vggiqdeevl ipellaeagy rnkivgkwhl ghqerylplr hgfhewfgss nchfgpydnv
      181 qrpniaffrd dhmigryfed fkinlttsts nltklyaqea vdfiekqaar rqpfflywap
      241 dashapvyss eefrsrsqrg pygdavmeld hsvgvildal rrtgtdnntl vfftsdngaa
      301 lvsktrggsn gpflcgkqtt fegglrepai awwpgtiaag keltgavatv ldllptlcel
      361 agvtlpptlv ldgasmvpll lgrlspadpr iqrdrpvffy rgnelmavrs gsykahfwtw
      421 tnsweefkqg idfcpgvvvd kvtsheqqnh talpvlfhlg rdpgekypln pkseeyrksm
      481 asilsiyheh kaalvpgkpq ldwcdasvmh wappgceeig rclrkppsrp ylcewph
//
LOCUS       XP_077503485             489 aa            linear   INV 25-NOV-2025
DEFINITION  N-acetylgalactosamine-6-sulfatase-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503485
VERSION     XP_077503485.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647359.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..489
                     /product="N-acetylgalactosamine-6-sulfatase-like isoform
                     X2"
                     /calculated_mol_wt=54174
     Region          37..461
                     /region_name="ALP_like"
                     /note="alkaline phosphatases and sulfatases; cl23718"
                     /db_xref="CDD:474031"
     Site            order(46,86,196,248..249)
                     /site_type="active"
                     /db_xref="CDD:293732"
     Site            order(46,86,196,249)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:293732"
     CDS             1..489
                     /gene="LOC144113945"
                     /coded_by="XM_077647359.1:117..1586"
                     /db_xref="GeneID:144113945"
ORIGIN      
        1 mwhhrleats iamlvhvcvc amlsttlaln ytdaspkpnf vimlmddmgw gdlgifgnpa
       61 ketpnldrma aegalltdfy tanplcspsr aalltgrlpi rngfyttnnh srngytpqei
      121 vggiqdeevl ipellaeagy rnkivgkwyf edfkinltts tsnltklyaq eavdfiekqa
      181 arrqpfflyw apdashapvy sseefrsrsq rgpygdavme ldhsvgvild alrrtgtdnn
      241 tlvfftsdng aalvsktrgg sngpflcgkq ttfegglrep aiawwpgtia agkeltgava
      301 tvldllptlc elagvtlppt lvldgasmvp lllgrlspad priqrdrpvf fyrgnelmav
      361 rsgsykahfw twtnsweefk qgidfcpgvv vdkvtsheqq nhtalpvlfh lgrdpgekyp
      421 lnpkseeyrk smasilsiyh ehkaalvpgk pqldwcdasv mhwappgcee igrclrkpps
      481 rpylcewph
//
LOCUS       XP_077503486             102 aa            linear   INV 25-NOV-2025
DEFINITION  N-acetylgalactosamine-6-sulfatase-like [Amblyomma americanum].
ACCESSION   XP_077503486
VERSION     XP_077503486.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647360.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..102
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..102
                     /product="N-acetylgalactosamine-6-sulfatase-like"
                     /calculated_mol_wt=11838
     Region          <3..>102
                     /region_name="ALP_like"
                     /note="alkaline phosphatases and sulfatases; cl23718"
                     /db_xref="CDD:474031"
     CDS             1..102
                     /gene="LOC144113946"
                     /coded_by="XM_077647360.1:312..620"
                     /db_xref="GeneID:144113946"
ORIGIN      
        1 mthlghqery lpsrhgfhew fgspnchfgp ydnvqrpnia fflddhmigr yfedfkinlt
       61 tstsnltkly aqeavdfiek qaarrqpffl ycapdashap vy
//
LOCUS       XP_077503487             430 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113948 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503487
VERSION     XP_077503487.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647361.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..430
                     /product="uncharacterized protein LOC144113948 isoform X1"
                     /calculated_mol_wt=46919
     Region          65..150
                     /region_name="CUB"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; cl00049"
                     /db_xref="CDD:412131"
     Region          169..198
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(172,180,191..192)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(184,187,191,197..198)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            194..198
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          <259..378
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..430
                     /gene="LOC144113948"
                     /coded_by="XM_077647361.1:375..1667"
                     /db_xref="GeneID:144113948"
ORIGIN      
        1 masvqrdhws fvvlqfllls llicrhgafa drnkyfhiss lcmgerhypq ykkidgavlt
       61 sesennleca vtfqtdsilq rfmlrferla ldchdhlvif dgahaignhk vdlscrstrs
      121 dvgtiftqgn yvtlkyttds wspqgngfkl iitaykdssl pdlkhdlkcr dfechnsfci
      181 ssnltcdgvn hcgdnsdets halcvddtas sqilglgvsv fvtlalvviv vclvlcvgtl
      241 clcqrlqqhq qqrqatsgam tlpgnhpaal qanhsafpre pllpshkvtr petaptgsse
      301 qglprdlsac reqfssprpl davppvspgg vtptstfrqg plssqpdlla aahgeapqht
      361 wantlprgrp dvlgpsfave aaqtlpwnmq ngtmlssstl rakgaelppd svrhhsvvss
      421 qpsgweqtwl
//
LOCUS       XP_077503488             328 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113948 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503488
VERSION     XP_077503488.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647362.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..328
                     /product="uncharacterized protein LOC144113948 isoform X2"
                     /calculated_mol_wt=35969
     Region          65..150
                     /region_name="CUB"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; cl00049"
                     /db_xref="CDD:412131"
     Region          169..198
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(172,180,191..192)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(184,187,191,197..198)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            194..198
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     CDS             1..328
                     /gene="LOC144113948"
                     /coded_by="XM_077647362.1:375..1361"
                     /db_xref="GeneID:144113948"
ORIGIN      
        1 masvqrdhws fvvlqfllls llicrhgafa drnkyfhiss lcmgerhypq ykkidgavlt
       61 sesennleca vtfqtdsilq rfmlrferla ldchdhlvif dgahaignhk vdlscrstrs
      121 dvgtiftqgn yvtlkyttds wspqgngfkl iitaykdssl pdlkhdlkcr dfechnsfci
      181 ssnltcdgvn hcgdnsdets halcvddtas sqilglgvsv fvtlalvviv vclvlcvgtl
      241 clcqrlqqhq qqrqatsgam tlpgnhpaal qanhsafpsh qpgsggvygg taerlcekpp
      301 pypgnsyppa agitfhvpqe slyypatk
//
LOCUS       XP_077503489             885 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113949 [Amblyomma americanum].
ACCESSION   XP_077503489
VERSION     XP_077503489.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647363.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..885
                     /product="uncharacterized protein LOC144113949"
                     /calculated_mol_wt=97065
     Region          601..621
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(601,604,617,621)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          631..652
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(631,634,648,652)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          660..680
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          707..728
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(707,710,723,728)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(712,714,716,718..719,722..723,727,741,743,747..748,
                     751..752,755,769,771,773,775..776,779..780,783)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          736..756
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          748..771
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          764..784
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          776..801
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          792..813
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          824..844
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..885
                     /gene="LOC144113949"
                     /coded_by="XM_077647363.1:166..2823"
                     /db_xref="GeneID:144113949"
ORIGIN      
        1 mdfgasstdi lncdisvssq esqiaqcler lespatiisg gtsaactqng tihvssiptw
       61 lsngmlgtst sdtqstgalp pvselrpgfr argnmqlwtl laqmasgqag pdtthvsapp
      121 ekhvddvhac taqnaaayhi dtcvktqatg qnaqhhsyet easysycdiq rsnisalepl
      181 sslgmdieqa hhtvaaldvd sitsayqqys cipllnipnn sisseqpssl ngilddalyc
      241 taksnhsips lpqvqtvqeq rqngspsnia kliassngss vqhvlnephv sdthkalshe
      301 aevatslisd tasttsgpvs arapataklp qpvssqsrdc srkndlttns fssnvqneqp
      361 aprhakktfs avnlveevvc yrckvcmflh esrsevikha eshrhgntqp sstmqrrptq
      421 qvpqqpanra ekrflcgkcr rgfftlddcc rhlsqahqak skkirlewiy tnqpdqmras
      481 tpaeeatlld pvpvgenapk qpvlpdftaa plddfphgap kqavkpvcta pkkkedsckt
      541 spsdlsssav sssqmawrqk inreqgsfic erkgcnvrfr elenleyhvr chradkpdfv
      601 cpecsvvclr wrtmtghlwr hhlcdmelhq cdqcdfrtys lsklenshkr thgeerpflc
      661 dvcgkgfktg kqlrnhktih rqasgqwstv aaaqgnggts slpaeacsec grcfrvrrll
      721 qlhrdsvhrk lrpflcsycg hrassqsslk mhlrqhtgek pfacnlceyr tgdhnslrrh
      781 kmrhsgtkpy kcphcpyaci qsstykshlh rkhpgqsdsl lfacplcsfr tvrednykah
      841 lsdhstsetq shngaaaaaa aeqssslsss alslgdivgf aqsas
//
LOCUS       XP_077503490             885 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113949 [Amblyomma americanum].
ACCESSION   XP_077503490
VERSION     XP_077503490.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647364.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..885
                     /product="uncharacterized protein LOC144113949"
                     /calculated_mol_wt=97065
     Region          601..621
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(601,604,617,621)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          631..652
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(631,634,648,652)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          660..680
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          707..728
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(707,710,723,728)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(712,714,716,718..719,722..723,727,741,743,747..748,
                     751..752,755,769,771,773,775..776,779..780,783)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          736..756
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          748..771
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          764..784
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          776..801
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          792..813
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          824..844
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..885
                     /gene="LOC144113949"
                     /coded_by="XM_077647364.1:244..2901"
                     /db_xref="GeneID:144113949"
ORIGIN      
        1 mdfgasstdi lncdisvssq esqiaqcler lespatiisg gtsaactqng tihvssiptw
       61 lsngmlgtst sdtqstgalp pvselrpgfr argnmqlwtl laqmasgqag pdtthvsapp
      121 ekhvddvhac taqnaaayhi dtcvktqatg qnaqhhsyet easysycdiq rsnisalepl
      181 sslgmdieqa hhtvaaldvd sitsayqqys cipllnipnn sisseqpssl ngilddalyc
      241 taksnhsips lpqvqtvqeq rqngspsnia kliassngss vqhvlnephv sdthkalshe
      301 aevatslisd tasttsgpvs arapataklp qpvssqsrdc srkndlttns fssnvqneqp
      361 aprhakktfs avnlveevvc yrckvcmflh esrsevikha eshrhgntqp sstmqrrptq
      421 qvpqqpanra ekrflcgkcr rgfftlddcc rhlsqahqak skkirlewiy tnqpdqmras
      481 tpaeeatlld pvpvgenapk qpvlpdftaa plddfphgap kqavkpvcta pkkkedsckt
      541 spsdlsssav sssqmawrqk inreqgsfic erkgcnvrfr elenleyhvr chradkpdfv
      601 cpecsvvclr wrtmtghlwr hhlcdmelhq cdqcdfrtys lsklenshkr thgeerpflc
      661 dvcgkgfktg kqlrnhktih rqasgqwstv aaaqgnggts slpaeacsec grcfrvrrll
      721 qlhrdsvhrk lrpflcsycg hrassqsslk mhlrqhtgek pfacnlceyr tgdhnslrrh
      781 kmrhsgtkpy kcphcpyaci qsstykshlh rkhpgqsdsl lfacplcsfr tvrednykah
      841 lsdhstsetq shngaaaaaa aeqssslsss alslgdivgf aqsas
//
LOCUS       XP_077503491             885 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113949 [Amblyomma americanum].
ACCESSION   XP_077503491
VERSION     XP_077503491.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647365.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..885
                     /product="uncharacterized protein LOC144113949"
                     /calculated_mol_wt=97065
     Region          601..621
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(601,604,617,621)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          631..652
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(631,634,648,652)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          660..680
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          707..728
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(707,710,723,728)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(712,714,716,718..719,722..723,727,741,743,747..748,
                     751..752,755,769,771,773,775..776,779..780,783)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          736..756
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          748..771
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          764..784
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          776..801
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          792..813
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          824..844
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..885
                     /gene="LOC144113949"
                     /coded_by="XM_077647365.1:166..2823"
                     /db_xref="GeneID:144113949"
ORIGIN      
        1 mdfgasstdi lncdisvssq esqiaqcler lespatiisg gtsaactqng tihvssiptw
       61 lsngmlgtst sdtqstgalp pvselrpgfr argnmqlwtl laqmasgqag pdtthvsapp
      121 ekhvddvhac taqnaaayhi dtcvktqatg qnaqhhsyet easysycdiq rsnisalepl
      181 sslgmdieqa hhtvaaldvd sitsayqqys cipllnipnn sisseqpssl ngilddalyc
      241 taksnhsips lpqvqtvqeq rqngspsnia kliassngss vqhvlnephv sdthkalshe
      301 aevatslisd tasttsgpvs arapataklp qpvssqsrdc srkndlttns fssnvqneqp
      361 aprhakktfs avnlveevvc yrckvcmflh esrsevikha eshrhgntqp sstmqrrptq
      421 qvpqqpanra ekrflcgkcr rgfftlddcc rhlsqahqak skkirlewiy tnqpdqmras
      481 tpaeeatlld pvpvgenapk qpvlpdftaa plddfphgap kqavkpvcta pkkkedsckt
      541 spsdlsssav sssqmawrqk inreqgsfic erkgcnvrfr elenleyhvr chradkpdfv
      601 cpecsvvclr wrtmtghlwr hhlcdmelhq cdqcdfrtys lsklenshkr thgeerpflc
      661 dvcgkgfktg kqlrnhktih rqasgqwstv aaaqgnggts slpaeacsec grcfrvrrll
      721 qlhrdsvhrk lrpflcsycg hrassqsslk mhlrqhtgek pfacnlceyr tgdhnslrrh
      781 kmrhsgtkpy kcphcpyaci qsstykshlh rkhpgqsdsl lfacplcsfr tvrednykah
      841 lsdhstsetq shngaaaaaa aeqssslsss alslgdivgf aqsas
//
LOCUS       XP_077503492             975 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113952 [Amblyomma americanum].
ACCESSION   XP_077503492
VERSION     XP_077503492.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647366.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..975
                     /product="uncharacterized protein LOC144113952"
                     /calculated_mol_wt=105200
     Region          558..580
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(558,563,576,580)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          <580..>746
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          620..640
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(620,623,636,640)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          648..668
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          685..706
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(685,688,701,706)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(690,692,694,696..697,700..701,705,719,721,725..726,
                     729..730,733,747,749,751,753..754,757..758,761)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          714..734
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          742..762
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          754..779
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          770..791
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          800..820
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..975
                     /gene="LOC144113952"
                     /coded_by="XM_077647366.1:157..3084"
                     /db_xref="GeneID:144113952"
ORIGIN      
        1 medlapqlsa ilvgeasgde sspqgtlhpg gisvpvtlvl ssglpeqadv etqsstmlnt
       61 lalhtsdgap pttqffleqg adgqtrlkta eghmlevdid pgtfglvqgn sglpvllqvs
      121 tdaalvhrdd vqiavaasgs gadsateste eqqkkpgsss aaceeteepm fqdgeqlplq
      181 mylqvgketa denstsledv vqtntgtava ivtpdgdesd adelptpnvv rrververle
      241 dfmdvvttyh ckfcsfscaw rsgvmshfrs chvapsgggs lepieppvns thkirpgpkv
      301 srtvnnavaf alttagpgln tavtctgaes gievdspate pavnasvepp epptsvpasv
      361 pasvpvseva skptteivps rvpmdaslvl enalasmast gssevlssss hpkprgesgq
      421 papaqerhif icgqcsqgfg slddckehmv eahdlkvgad sdvetkkavt srkrgrprct
      481 aqlsestsgt acttttgsdv iagalqsvdd gdledgsnea lgkrrvrppk nleddylvap
      541 arrrrakken aaerqyrcge rscgyrfrte qnleyhvrch tpsasrpycc pecgeqrdhw
      601 rslamhlwrs hlldldlhrc tqcnyrtfsa fklenharih seerdftcaq cgkgfkqlsq
      661 lrnhhvvhld rkntphkrwy sqqtcelcqr tfsdskclrk hhqavhgkvk pyvcsfcghm
      721 sarkamlqlh lrqhtgekpf acnlceyrtg dhnslrrhkm rhsgtkpykc phcpyaciqa
      781 isykmhmknk hpgldglyac slcsfrsvsk enytnhmsdh krgflavpaq lsspstanst
      841 astegeglql pegtlqqleg ilpgnlnaaq liysclsalq qeggsanlpp gvttystgdg
      901 tqtitiqvps taagedsgss llpapseqdq fyltvqqqed gsmaylsgeg faqgadmddd
      961 vaslkisavh ftesr
//
LOCUS       XP_077503493             975 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113952 [Amblyomma americanum].
ACCESSION   XP_077503493
VERSION     XP_077503493.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647367.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..975
                     /product="uncharacterized protein LOC144113952"
                     /calculated_mol_wt=105200
     Region          558..580
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(558,563,576,580)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          <580..>746
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          620..640
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(620,623,636,640)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          648..668
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          685..706
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(685,688,701,706)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(690,692,694,696..697,700..701,705,719,721,725..726,
                     729..730,733,747,749,751,753..754,757..758,761)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          714..734
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          742..762
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          754..779
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          770..791
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          800..820
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..975
                     /gene="LOC144113952"
                     /coded_by="XM_077647367.1:175..3102"
                     /db_xref="GeneID:144113952"
ORIGIN      
        1 medlapqlsa ilvgeasgde sspqgtlhpg gisvpvtlvl ssglpeqadv etqsstmlnt
       61 lalhtsdgap pttqffleqg adgqtrlkta eghmlevdid pgtfglvqgn sglpvllqvs
      121 tdaalvhrdd vqiavaasgs gadsateste eqqkkpgsss aaceeteepm fqdgeqlplq
      181 mylqvgketa denstsledv vqtntgtava ivtpdgdesd adelptpnvv rrververle
      241 dfmdvvttyh ckfcsfscaw rsgvmshfrs chvapsgggs lepieppvns thkirpgpkv
      301 srtvnnavaf alttagpgln tavtctgaes gievdspate pavnasvepp epptsvpasv
      361 pasvpvseva skptteivps rvpmdaslvl enalasmast gssevlssss hpkprgesgq
      421 papaqerhif icgqcsqgfg slddckehmv eahdlkvgad sdvetkkavt srkrgrprct
      481 aqlsestsgt acttttgsdv iagalqsvdd gdledgsnea lgkrrvrppk nleddylvap
      541 arrrrakken aaerqyrcge rscgyrfrte qnleyhvrch tpsasrpycc pecgeqrdhw
      601 rslamhlwrs hlldldlhrc tqcnyrtfsa fklenharih seerdftcaq cgkgfkqlsq
      661 lrnhhvvhld rkntphkrwy sqqtcelcqr tfsdskclrk hhqavhgkvk pyvcsfcghm
      721 sarkamlqlh lrqhtgekpf acnlceyrtg dhnslrrhkm rhsgtkpykc phcpyaciqa
      781 isykmhmknk hpgldglyac slcsfrsvsk enytnhmsdh krgflavpaq lsspstanst
      841 astegeglql pegtlqqleg ilpgnlnaaq liysclsalq qeggsanlpp gvttystgdg
      901 tqtitiqvps taagedsgss llpapseqdq fyltvqqqed gsmaylsgeg faqgadmddd
      961 vaslkisavh ftesr
//
LOCUS       XP_077503495             127 aa            linear   INV 25-NOV-2025
DEFINITION  NADH dehydrogenase (ubiquinone) 13 kDa A subunit [Amblyomma
            americanum].
ACCESSION   XP_077503495
VERSION     XP_077503495.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647369.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="NADH dehydrogenase (ubiquinone) 13 kDa A
                     subunit"
                     /calculated_mol_wt=14207
     Region          82..118
                     /region_name="zf-CHCC"
                     /note="Zinc-finger domain; pfam10276"
                     /db_xref="CDD:463041"
     CDS             1..127
                     /gene="ND-13A"
                     /coded_by="XM_077647369.1:129..512"
                     /db_xref="GeneID:144113953"
ORIGIN      
        1 mnscvrnpsr fvqitqrlav hyrlssnvvk dveppkdpvt htgqhwdked yrlvrfmenp
       61 kqvnpnfavk liaevppkav narttwcdgg ngalghprvf inldapgnhs cgycglrfyq
      121 dhsskgh
//
LOCUS       XP_077503496             333 aa            linear   INV 25-NOV-2025
DEFINITION  F-box/LRR-repeat protein 12-like [Amblyomma americanum].
ACCESSION   XP_077503496
VERSION     XP_077503496.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647370.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..333
                     /product="F-box/LRR-repeat protein 12-like"
                     /calculated_mol_wt=37022
     Region          7..50
                     /region_name="F-box-like"
                     /note="pfam12937"
                     /db_xref="CDD:463757"
     Region          <47..233
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     Region          102..129
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          130..153
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          154..178
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          <175..286
                     /region_name="AMN1"
                     /note="Antagonist of mitotic exit network protein 1;
                     cd09293"
                     /db_xref="CDD:187754"
     Region          179..204
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          205..229
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          230..255
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          256..280
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     CDS             1..333
                     /gene="LOC144113955"
                     /coded_by="XM_077647370.1:134..1135"
                     /db_xref="GeneID:144113955"
ORIGIN      
        1 mtafdadalp dvvllhifrl lkfsdlckvg rvcrrwygls rsqalwtcvd asapeissvq
       61 mdrlasvltd svrwlyvssf lhcglflspg alrdlrahcp ylstlviksa llttlddfap
      121 vtvgdfpptl ktlslrhsff qvdqffslla irnlsildls fcwcvsdqdi pfvtqlpnlr
      181 elyleycedi rdtgvalvvs rghnlktlal dgtkitdeai rtiaenapal erlyvgstav
      241 tdvgvlhlck gkcfslqelc lfstrvtvqt ikvlvdifpq lqwlnlehtl idigdspliv
      301 kqvppgctvl vdgvrfhlgv gcnhsvdrlr lqd
//
LOCUS       XP_077503497             254 aa            linear   INV 25-NOV-2025
DEFINITION  cleavage and polyadenylation specificity factor subunit 5 isoform
            X1 [Amblyomma americanum].
ACCESSION   XP_077503497
VERSION     XP_077503497.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647371.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..254
                     /product="cleavage and polyadenylation specificity factor
                     subunit 5 isoform X1"
                     /calculated_mol_wt=28699
     Region          55..236
                     /region_name="NUDIX_Cfim25_Nudt21"
                     /note="25 kDa subunit of cleavage factor Im; cd18871"
                     /db_xref="CDD:467583"
     CDS             1..254
                     /gene="Cpsf5"
                     /coded_by="XM_077647371.1:18..782"
                     /db_xref="GeneID:144113956"
ORIGIN      
        1 mremrpersk mashitrsgs agwpksapqg qytgkymgsn panllnftln rtvnlypltn
       61 ytfgtkealy erdssvparf qrmreefeki gmrrsvegvl lvhehglphv lllqlgttff
      121 klpggelnmg edevdglkrl mteilgrqdg vkqdwviedt vgnwwrpnfe ppqypyvpph
      181 itkpkehkrl ylvqlpekal favpknyklv aaplfelydn aqgygpiiss lpqalsskil
      241 glsffadstl sicd
//
LOCUS       XP_077503498             243 aa            linear   INV 25-NOV-2025
DEFINITION  cleavage and polyadenylation specificity factor subunit 5 isoform
            X2 [Amblyomma americanum].
ACCESSION   XP_077503498
VERSION     XP_077503498.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647372.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..243
                     /product="cleavage and polyadenylation specificity factor
                     subunit 5 isoform X2"
                     /calculated_mol_wt=27754
     Region          55..237
                     /region_name="NUDIX_Cfim25_Nudt21"
                     /note="25 kDa subunit of cleavage factor Im; cd18871"
                     /db_xref="CDD:467583"
     CDS             1..243
                     /gene="Cpsf5"
                     /coded_by="XM_077647372.1:17..748"
                     /db_xref="GeneID:144113956"
ORIGIN      
        1 mremrpersk mashitrsgs agwpksapqg qytgkymgsn panllnftln rtvnlypltn
       61 ytfgtkealy erdssvparf qrmreefeki gmrrsvegvl lvhehglphv lllqlgttff
      121 klpggelnmg edevdglkrl mteilgrqdg vkqdwviedt vgnwwrpnfe ppqypyvpph
      181 itkpkehkrl ylvqlpekal favpknyklv aaplfelydn aqgygpiiss lpqalsrfnf
      241 iyl
//
LOCUS       XP_077503499             199 aa            linear   INV 25-NOV-2025
DEFINITION  cleavage and polyadenylation specificity factor subunit 5 isoform
            X3 [Amblyomma americanum].
ACCESSION   XP_077503499
VERSION     XP_077503499.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647373.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..199
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..199
                     /product="cleavage and polyadenylation specificity factor
                     subunit 5 isoform X3"
                     /calculated_mol_wt=22794
     Region          55..>198
                     /region_name="NUDIX_Cfim25_Nudt21"
                     /note="25 kDa subunit of cleavage factor Im; cd18871"
                     /db_xref="CDD:467583"
     CDS             1..199
                     /gene="Cpsf5"
                     /coded_by="XM_077647373.1:17..616"
                     /db_xref="GeneID:144113956"
ORIGIN      
        1 mremrpersk mashitrsgs agwpksapqg qytgkymgsn panllnftln rtvnlypltn
       61 ytfgtkealy erdssvparf qrmreefeki gmrrsvegvl lvhehglphv lllqlgttff
      121 klpggelnmg edevdglkrl mteilgrqdg vkqdwviedt vgnwwrpnfe ppqypyvpph
      181 itkpkehkrl ylvqlpekg
//
LOCUS       XP_077503500             361 aa            linear   INV 25-NOV-2025
DEFINITION  putative tRNA N6-adenosine threonylcarbamoyltransferase Tcs3
            isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503500
VERSION     XP_077503500.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647374.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..361
                     /product="putative tRNA N6-adenosine
                     threonylcarbamoyltransferase Tcs3 isoform X1"
                     /calculated_mol_wt=38902
     Region          3..310
                     /region_name="ASKHA_NBD_OSGEP_like_euk"
                     /note="nucleotide-binding domain (NBD) of
                     O-sialoglycoprotein endopeptidase (OSGEP) and similar
                     proteins mainly from eukaryotes; cd24132"
                     /db_xref="CDD:466982"
     CDS             1..361
                     /gene="Tcs3"
                     /coded_by="XM_077647374.1:94..1179"
                     /db_xref="GeneID:144113960"
ORIGIN      
        1 mtvaigfegs anklgvgivr dgevlsnprv tyitppgegf qprdtaihhr ahvldvlera
       61 leeaniapnq idvvcytkgp glgaplvsva vvartvaqiw nkpligvnhc ighiemgrli
      121 tgavnptvly vsggntqvia ysekryrifg etidiavgnc ldrfarvlkl sndpspgyni
      181 eqmakrgtkl vplpyvvkgm dvsfsgllsf ieeraeslls kgectaedlc fslqetvfam
      241 lvetterama htgssevliv ggvgcnkrlq emmgimaeer saklfatder fcidngamia
      301 qagwemfrsg qvtcfedttc tqrcmrdfpr dkqhspaaqg qscpgglepq eisgpgngha
      361 l
//
LOCUS       XP_077503501             335 aa            linear   INV 25-NOV-2025
DEFINITION  putative tRNA N6-adenosine threonylcarbamoyltransferase Tcs3
            isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503501
VERSION     XP_077503501.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647375.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..335
                     /product="putative tRNA N6-adenosine
                     threonylcarbamoyltransferase Tcs3 isoform X2"
                     /calculated_mol_wt=36496
     Region          3..310
                     /region_name="ASKHA_NBD_OSGEP_like_euk"
                     /note="nucleotide-binding domain (NBD) of
                     O-sialoglycoprotein endopeptidase (OSGEP) and similar
                     proteins mainly from eukaryotes; cd24132"
                     /db_xref="CDD:466982"
     CDS             1..335
                     /gene="Tcs3"
                     /coded_by="XM_077647375.1:94..1101"
                     /db_xref="GeneID:144113960"
ORIGIN      
        1 mtvaigfegs anklgvgivr dgevlsnprv tyitppgegf qprdtaihhr ahvldvlera
       61 leeaniapnq idvvcytkgp glgaplvsva vvartvaqiw nkpligvnhc ighiemgrli
      121 tgavnptvly vsggntqvia ysekryrifg etidiavgnc ldrfarvlkl sndpspgyni
      181 eqmakrgtkl vplpyvvkgm dvsfsgllsf ieeraeslls kgectaedlc fslqetvfam
      241 lvetterama htgssevliv ggvgcnkrlq emmgimaeer saklfatder fcidngamia
      301 qagwemfrsg qvtcfedttc tqryrtdeve vtwrd
//
LOCUS       XP_077503502            1008 aa            linear   INV 25-NOV-2025
DEFINITION  meckelin [Amblyomma americanum].
ACCESSION   XP_077503502
VERSION     XP_077503502.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647376.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1008
                     /product="meckelin"
                     /calculated_mol_wt=113256
     Region          57..186
                     /region_name="TNFRSF"
                     /note="Tumor necrosis factor receptor superfamily
                     (TNFRSF); cl22855"
                     /db_xref="CDD:473981"
     Region          85..115
                     /region_name="CRD2"
                     /note="CRD2 [structural motif]"
                     /db_xref="CDD:276930"
     Region          149..1007
                     /region_name="Meckelin"
                     /note="Meckelin (Transmembrane protein 67); pfam09773"
                     /db_xref="CDD:462892"
     CDS             1..1008
                     /gene="LOC144113961"
                     /coded_by="XM_077647376.1:123..3149"
                     /db_xref="GeneID:144113961"
ORIGIN      
        1 mlfivfigal ivfaesryls ayrtpsecdn asyfdvvals cvkcganrgq ehmqlaskdg
       61 sscqckpqhk iislesatki sclscppehv tsldgwdcvl cadsspfqkt tnscsscvdg
      121 vyeeivgsdg irrrtctacs ertwpdvnsq kcvtcpeefl lanssacscp sdkyfrvags
      181 cipaakvdan fdalynvkhq dssissgalr slllpslync kehgnetace alanlcvltl
      241 ylrssegtic drfltlgeas srrpkqsnqg lpwlfylddd anliisrtdv pnqftikhwk
      301 nssylhfiva kysltgklia igplkirels lcdtslankd ilrfgttfne ecqkparglw
      361 desklvfydv slesrqngik kiypvpvkie nleknsrrhs eegalswqlv rrfflvdpvt
      421 gagsdssprn vrpsakflry aekinihitl hkkgkpgsih pplltvryae aswdayeqda
      481 tlpisfsity ssdysqaaqd mpialgvlsa lavlwaciqt wswtrrsgrv sinvvtlvkl
      541 itytcsslss vflivvlstc lnwllmfkfq dvvhmllpsp hqerdiklyi ifafvmktfy
      601 llhvvflqss adiffldwer phamariprp grkdtsrlsv agdasqatgs sdagssisva
      661 adlkgggesg tavsiwrsyf vanewcelqc hrrvslslql lalllllkgl gleyvalasp
      721 esyyarslkn anipfsaacr falaaslyll iallqvvlqk fiyerfyedk lqhfvdlcsv
      781 snisvvifvq pkfgyyihgr saqghadvsm kemhellrre eddlcghrgl lpdteqqtfq
      841 mtlpshiyeq ycrmrrplyt ytqapdriqt adghlsrvni dtvvniynvv tkflsafldh
      901 slkdidytvk drtvfeklld iefndavdrg ffyndnghsf daviyhghef tlvlwdllmf
      961 cvadfassdf vlaaaityvv dkmleglrry lsrrnlvkka lvderflw
//
LOCUS       XP_077503504             578 aa            linear   INV 25-NOV-2025
DEFINITION  luciferin 4-monooxygenase-like [Amblyomma americanum].
ACCESSION   XP_077503504
VERSION     XP_077503504.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647378.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..578
                     /product="luciferin 4-monooxygenase-like"
                     /calculated_mol_wt=61591
     Region          80..562
                     /region_name="AFD_class_I"
                     /note="Adenylate forming domain, Class I superfamily;
                     cl17068"
                     /db_xref="CDD:473059"
     Site            order(226,229..234,236..237)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341228"
     Site            order(229,343..344,366..371,450,462,465,476,560)
                     /site_type="other"
                     /note="AMP binding site [chemical binding]"
                     /db_xref="CDD:341228"
     Site            order(229,269..270,316,318..319,322,343..344,366..371,450,
                     462,465,473..476,541)
                     /site_type="active"
                     /db_xref="CDD:341228"
     Site            order(269,318..319,322,343,473..475,534,541)
                     /site_type="other"
                     /note="CoA binding site [chemical binding]"
                     /db_xref="CDD:341228"
     CDS             1..578
                     /gene="LOC144113962"
                     /coded_by="XM_077647378.1:137..1873"
                     /db_xref="GeneID:144113962"
ORIGIN      
        1 mgttaeesgw tcklpqkdre csmadsgapq pkasqqaarg paaivedrvv rsavpepplp
       61 dcdfatclrn swqqfkdrta liehstderc syeqleeqcs rvaaglrslg fgpgdmagfh
      121 cgssldaiia cygvvlaggc vvmakpnlsq relsyqfgds spslvfsdee ltdkviavqk
      181 evpsiktlvv fgnhaaalpf aplkktplse cqapqapadp gsllfvlyss gttgkpkgvm
      241 ishrnavaqf tavgappkpp ippvvlgvtp fthasgvlmv navfargdsl vilscpepsl
      301 vipaiakhka vfmfefpthl rlllksplle dydlsslkvv svagstvppd lvtetkaklk
      361 vfalaqgygl tesfgagtft lgqnassdcv gtphfmsqlk ivdittgekl gpgqrgeiri
      421 rspcccigyl nnpeasaaly deegflksgd mgyyneqgvf yitdrvkdli kcmdqqvppa
      481 eleglllqhe avldvavvgv phddygeaar afvvlreghe psqalkdalf nlvadqasyh
      541 khlhggvefa asipksftgk plrsslrdah akqaqasp
//
LOCUS       XP_077503505            1192 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: annulin-like [Amblyomma americanum].
ACCESSION   XP_077503505
VERSION     XP_077503505.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647379.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1192
                     /product="LOW QUALITY PROTEIN: annulin-like"
                     /calculated_mol_wt=131874
     Region          480..602
                     /region_name="Transglut_N"
                     /note="Transglutaminase family; pfam00868"
                     /db_xref="CDD:459971"
     Region          751..843
                     /region_name="TGc"
                     /note="Transglutaminase/protease-like homologues;
                     smart00460"
                     /db_xref="CDD:214673"
     Region          962..1068
                     /region_name="Transglut_C"
                     /note="Transglutaminase family, C-terminal ig like domain;
                     pfam00927"
                     /db_xref="CDD:460002"
     Region          1076..1172
                     /region_name="Transglut_C"
                     /note="Transglutaminase family, C-terminal ig like domain;
                     pfam00927"
                     /db_xref="CDD:460002"
     CDS             1..1192
                     /gene="LOC144113963"
                     /coded_by="XM_077647379.1:16..3594"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:144113963"
ORIGIN      
        1 mdvprsvrfr shsqdarprr gprrtpdsee qspppvpkhg passppkknr pknlkvafes
       61 vdidsrlsaf hsengsarfr appsrshrle dftqvhsgrr rgvasaepsp rptrrasvss
      121 vasddgcyrr mgdvvsrrtf ptvepvagda vyanvpllvr geepplpapl qvspvgssre
      181 sigsahvygh rgalapklkn kdaqtgaaes gekstkvtkp kpldflpwcl cskgstgrts
      241 lpygkprqrp rlpavteeas rrnstdigtp egelgflqhl clcavcraqm qscqllaqlv
      301 seaqgaagpl aaiqspmgyg lyrsrqptaa ppsdsttsee mhdsfesgvr deynflrepe
      361 sedstfyddd aepvhlpeqa cfkedsgggg gggsgeealr hppwsvsvgg rssaakstsn
      421 akssaspaeq rrrgamgsvl scfhgsfsps eaetehkllp vapkppadgk gdsivqvrev
      481 nlrvaenaqq hrtedyeltk qpnpvlvvrr gdpfkvaiel srhynpdrda lsfvftvrge
      541 eapsytkntv avvpvgfhss aeflgsitgw talvqntrdt vldieitppp nvivgdwimd
      601 idskirsend ntgfrythkd pvyilfnpwn radtvymdht gmryecvlme sgliwrgshs
      661 rlrptvwsyg qfeknvlecc vyllrhvgrl pspacadpvk varhvsavvn apndegvivg
      721 nwtekfsngt pptgwmgsas ilqqfykskk pvkygqcwvf agvattvlra lgipsrpvtn
      781 yysahdthss ltvdqfyddn geaieklnkd siwnfhvwne awmtrpdlep gdysgwqaid
      841 atpqeasdgv yrcgpasvka ikraeilkpy dgtfifaevn adqiywryqg pmqplklirk
      901 atdkigqcis tkaigkfere ditdsykhpe htqeernaml kalrkcnhay sryylndele
      961 dvsfdfelld diiigspfsv klsarnknpt keytahvvmr vesthytgnk kklvksgqfd
     1021 vaikpnsree islevtypey ekllldqamf niatlatike tkfdyfaqdd frvrmpdvki
     1081 epegdalvkk plkvkatfrn plpkpltkgm fviegpglpe plrlpvkqki hageevtinf
     1141 yltpkaagpk aiiakfssre lsdvdgfkav dvrepsfles tnmvtmpiaa ta
//
LOCUS       XP_077503506             736 aa            linear   INV 25-NOV-2025
DEFINITION  annulin-like [Amblyomma americanum].
ACCESSION   XP_077503506
VERSION     XP_077503506.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647380.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..736
                     /product="annulin-like"
                     /calculated_mol_wt=82355
     Region          27..150
                     /region_name="Transglut_N"
                     /note="Transglutaminase family; pfam00868"
                     /db_xref="CDD:459971"
     Region          299..391
                     /region_name="TGc"
                     /note="Transglutaminase/protease-like homologues;
                     smart00460"
                     /db_xref="CDD:214673"
     Region          510..616
                     /region_name="Transglut_C"
                     /note="Transglutaminase family, C-terminal ig like domain;
                     pfam00927"
                     /db_xref="CDD:460002"
     Region          624..721
                     /region_name="Transglut_C"
                     /note="Transglutaminase family, C-terminal ig like domain;
                     pfam00927"
                     /db_xref="CDD:460002"
     CDS             1..736
                     /gene="LOC144113964"
                     /coded_by="XM_077647380.1:322..2532"
                     /db_xref="GeneID:144113964"
ORIGIN      
        1 mgdvssrlqg nddegqpgrp devvkvrsvd myvtynaenh ftrgfartks qnpapvlrrg
       61 qsftmvinln rpfnpssdal slffrlegvd ratpysketv slvpvdstga ealtpgawsa
      121 airkteeksl lvevmapvtc ivgswymdvd sklrsamdss gstytypdpl yilfnpwskd
      181 dtvymenaeg rfeyvlahtg iiwrgsynsl rpspwkyghf eenvldcclh llrnvgklpv
      241 qdcanpvlva rhisavvnss ddqgvlmgnw tsnfaggvpp vswtgshpil qqffktrkpv
      301 kygqcwvfag vttavcralg ipartvtnyy sahdthasat vdqfyddqgk aveklntdsi
      361 wnfhvwnevw mkrpdlgkee ydgwqvidat pqemsggifs cgpasvkaik agdlakpydc
      421 tfvfaevnad qiywryespr kplkmlkkst dkvgqhlstk avgrwdredi theykfpegt
      481 ererdivlka lrdnnhaysr fylndsmddv qfniepikdv lvgsgivvra kavnksaskr
      541 yackvilrve tvnytgvtrr alkhqnftln lqpgaeemit levgykdysr dvsdqasfkv
      601 tmmgnvpevk ydyivqedfm ltmpdieiks ygpvvvgkrq kftasfqnpl ptrltrcffa
      661 vegpglsrpy yislrepvqp rseawiefal tpatsgprsi aakfsstqla dvegfmevvv
      721 kgeeatpdaa pngtte
//
LOCUS       XP_077503507             212 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113965 [Amblyomma americanum].
ACCESSION   XP_077503507
VERSION     XP_077503507.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647381.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..212
                     /product="uncharacterized protein LOC144113965"
                     /calculated_mol_wt=22477
     CDS             1..212
                     /gene="LOC144113965"
                     /coded_by="XM_077647381.1:126..764"
                     /db_xref="GeneID:144113965"
ORIGIN      
        1 mragrlllpa aawlaalhtv alatsryvpd apggvrtaga avrnvgsssy tatgvlhlae
       61 ssppevvgts rrraaprgga lertgagdfg vsyigsppgs eetadwtdlv vtklrmqakp
      121 slgfeprptr hrrppaapar rrplhvprfa taagggsvgr saraksprft ylktvsyttf
      181 pyygfgygpy gfgyswkpyr pwgmmygamg gf
//
LOCUS       XP_077503508             396 aa            linear   INV 25-NOV-2025
DEFINITION  homocysteine-induced endoplasmic reticulum protein [Amblyomma
            americanum].
ACCESSION   XP_077503508
VERSION     XP_077503508.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647382.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..396
                     /product="homocysteine-induced endoplasmic reticulum
                     protein"
                     /calculated_mol_wt=42647
     Region          10..84
                     /region_name="Ubl_HERP"
                     /note="ubiquitin-like (Ubl) domain found in
                     homocysteine-inducible endoplasmic reticulum stress
                     protein HERP; cd01790"
                     /db_xref="CDD:340488"
     CDS             1..396
                     /gene="Herp"
                     /coded_by="XM_077647382.1:176..1366"
                     /db_xref="GeneID:144113966"
ORIGIN      
        1 maaqnmstcl virapnqkig dqqvycsldw svqqlkqhls kvypnnpkpe dqkliysgql
       61 ledemtlkdv fqmdfgkihi lhlvcrpggd npeaspssvp lvalsqpdvn aaggsvstvp
      121 stssvnsass dglrhrstsp gavpngqmpi algmppgass peqlvqqval aqqlyahyft
      181 qymhlmhqaa apvaaqasap rppspeaeap gapaaappvp raapdnrgpr mnaqggplld
      241 eeeeeaaqrd wldwvytvsr atvllsivyf yssfgrflvv tgiallmyly qggwfaapqq
      301 qqhperghdr nrareannqq hqaghpagdp qempqgetgp qqleeamdqd ddgimapptp
      361 ppppeppvsp mttawtfvat fftslipeqp ppvnan
//
LOCUS       XP_077503509             396 aa            linear   INV 25-NOV-2025
DEFINITION  homocysteine-induced endoplasmic reticulum protein [Amblyomma
            americanum].
ACCESSION   XP_077503509
VERSION     XP_077503509.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647383.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..396
                     /product="homocysteine-induced endoplasmic reticulum
                     protein"
                     /calculated_mol_wt=42647
     Region          10..84
                     /region_name="Ubl_HERP"
                     /note="ubiquitin-like (Ubl) domain found in
                     homocysteine-inducible endoplasmic reticulum stress
                     protein HERP; cd01790"
                     /db_xref="CDD:340488"
     CDS             1..396
                     /gene="Herp"
                     /coded_by="XM_077647383.1:260..1450"
                     /db_xref="GeneID:144113966"
ORIGIN      
        1 maaqnmstcl virapnqkig dqqvycsldw svqqlkqhls kvypnnpkpe dqkliysgql
       61 ledemtlkdv fqmdfgkihi lhlvcrpggd npeaspssvp lvalsqpdvn aaggsvstvp
      121 stssvnsass dglrhrstsp gavpngqmpi algmppgass peqlvqqval aqqlyahyft
      181 qymhlmhqaa apvaaqasap rppspeaeap gapaaappvp raapdnrgpr mnaqggplld
      241 eeeeeaaqrd wldwvytvsr atvllsivyf yssfgrflvv tgiallmyly qggwfaapqq
      301 qqhperghdr nrareannqq hqaghpagdp qempqgetgp qqleeamdqd ddgimapptp
      361 ppppeppvsp mttawtfvat fftslipeqp ppvnan
//
LOCUS       XP_077503510             396 aa            linear   INV 25-NOV-2025
DEFINITION  homocysteine-induced endoplasmic reticulum protein [Amblyomma
            americanum].
ACCESSION   XP_077503510
VERSION     XP_077503510.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647384.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..396
                     /product="homocysteine-induced endoplasmic reticulum
                     protein"
                     /calculated_mol_wt=42647
     Region          10..84
                     /region_name="Ubl_HERP"
                     /note="ubiquitin-like (Ubl) domain found in
                     homocysteine-inducible endoplasmic reticulum stress
                     protein HERP; cd01790"
                     /db_xref="CDD:340488"
     CDS             1..396
                     /gene="Herp"
                     /coded_by="XM_077647384.1:51..1241"
                     /db_xref="GeneID:144113966"
ORIGIN      
        1 maaqnmstcl virapnqkig dqqvycsldw svqqlkqhls kvypnnpkpe dqkliysgql
       61 ledemtlkdv fqmdfgkihi lhlvcrpggd npeaspssvp lvalsqpdvn aaggsvstvp
      121 stssvnsass dglrhrstsp gavpngqmpi algmppgass peqlvqqval aqqlyahyft
      181 qymhlmhqaa apvaaqasap rppspeaeap gapaaappvp raapdnrgpr mnaqggplld
      241 eeeeeaaqrd wldwvytvsr atvllsivyf yssfgrflvv tgiallmyly qggwfaapqq
      301 qqhperghdr nrareannqq hqaghpagdp qempqgetgp qqleeamdqd ddgimapptp
      361 ppppeppvsp mttawtfvat fftslipeqp ppvnan
//
LOCUS       XP_077503511             591 aa            linear   INV 25-NOV-2025
DEFINITION  alcohol dehydrogenase [acceptor]-like [Amblyomma americanum].
ACCESSION   XP_077503511
VERSION     XP_077503511.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647385.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..591
                     /product="alcohol dehydrogenase [acceptor]-like"
                     /calculated_mol_wt=64652
     Region          35..588
                     /region_name="BetA"
                     /note="Choline dehydrogenase or related flavoprotein
                     [Lipid transport and metabolism, General function
                     prediction only]; COG2303"
                     /db_xref="CDD:441878"
     CDS             1..591
                     /gene="LOC144113968"
                     /coded_by="XM_077647385.1:395..2170"
                     /db_xref="GeneID:144113968"
ORIGIN      
        1 mrlgkkelvl lnlvcigtav lwhhlrrcqd irlppdpeyd yivvgagtag cvlarrllds
       61 asgadgsaar vllieagrge apwyasvpll slamqatsid wqfvtapqkd alhcyhnqsl
      121 rwprgkalgg ssnlyfmihs rpapedwelw sqrcracrfr adrvdalyrk lefpaghgha
      181 drvldaripv ldtncgasel csafkeaagr lglgldkeen qllksnvyqg rrwttydlyi
      241 kpvltnprlr imtgahvsrv lfenttavgv eiiadgvvyq agakreviis sgavgsphil
      301 llsgigpkda ldkfqipvvs elpgvgnnlq dhvtvpiyfn lkapvsiney ktkslwqiwk
      361 yiwgeghlas sgvasvlrts igdrhleamf lvinfgsfre dilsrishmk kedidasfpd
      421 ignankegfl llascrhpks kgritlhtgh aldspnidpr yfsdphdirc vtnamttala
      481 lasdkaisal gasphiprlr ycehldasvq nddflecwar taagslhhpv gtaamgdasd
      541 ssavvdelfr vrgverlrvv dasvipfals stphsvvvml aeqaaemisa p
//
LOCUS       XP_077503512             353 aa            linear   INV 25-NOV-2025
DEFINITION  G protein alpha o subunit [Amblyomma americanum].
ACCESSION   XP_077503512
VERSION     XP_077503512.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647386.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..353
                     /product="G protein alpha o subunit"
                     /calculated_mol_wt=40086
     Region          34..347
                     /region_name="G-alpha"
                     /note="Alpha subunit of G proteins (guanine nucleotide
                     binding); cd00066"
                     /db_xref="CDD:206639"
     Site            order(40,45..47,181,200,203,269..270,272,324..325)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206639"
     Site            40..47
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206639"
     Site            order(41..43,71,75,79,82,85..86,104,107,149,178,203,211,
                     215)
                     /site_type="active"
                     /note="GoLoco binding site [active]"
                     /db_xref="CDD:206639"
     Site            176..184
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206639"
     Site            181
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206639"
     Site            order(182,184,186,197,199,203..205,207,210..211,213..216)
                     /site_type="other"
                     /note="beta - gamma complex interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206639"
     Site            order(184,209,212..213,216)
                     /site_type="other"
                     /note="adenylyl cyclase interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206639"
     Site            200..203
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206639"
     Site            201..217
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206639"
     Site            269..272
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206639"
     Site            order(316..327,329..332)
                     /site_type="active"
                     /note="putative receptor binding site [active]"
                     /db_xref="CDD:206639"
     Site            324..326
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206639"
     CDS             1..353
                     /gene="Galphao"
                     /coded_by="XM_077647386.1:190..1251"
                     /db_xref="GeneID:144113969"
ORIGIN      
        1 mgcamsaeer aalarskqie knlkedgiqa akdikllllg agesgkstiv kqmkiihdsg
       61 ftqedfkqyk pvvysntiqs mvailrampn lgisfgnner evrsffentl fsktekgnln
      121 pehdtktilk elwvpgqkcf sfntstqlnn safaflddld rlgkkeympt eqdilrtrvk
      181 ttgivevhfs fknlnfklfd vggqrserkk wihcfedvta iifcvamsey dqvlhedett
      241 nrmqeslklf dsicnnkwft dtsiilflnk kdlfeekikk splticfpey tgaqeygeaa
      301 ayiqaqfeak nksttkeiyc hmtcatdttn iqfvfdavtd viiannlrgc gly
//
LOCUS       XP_077503513              89 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113970 [Amblyomma americanum].
ACCESSION   XP_077503513
VERSION     XP_077503513.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647387.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..89
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..89
                     /product="uncharacterized protein LOC144113970"
                     /calculated_mol_wt=9479
     CDS             1..89
                     /gene="LOC144113970"
                     /coded_by="XM_077647387.1:414..683"
                     /db_xref="GeneID:144113970"
ORIGIN      
        1 massggakvc wmlvwlllli faafwvaafa afwyilvyvl vpcvpaakdv aallhrgvll
       61 pyfcsdnlvn grdlnsgvqf icsggtvpt
//
LOCUS       XP_077503515             521 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113971 [Amblyomma americanum].
ACCESSION   XP_077503515
VERSION     XP_077503515.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647389.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..521
                     /product="uncharacterized protein LOC144113971"
                     /calculated_mol_wt=58366
     CDS             1..521
                     /gene="LOC144113971"
                     /coded_by="XM_077647389.1:523..2088"
                     /db_xref="GeneID:144113971"
ORIGIN      
        1 mallyrftrl ndrsntgvft fiatrsvtrd lhrdattkdf pfayhrwavs fvrtdkvlgv
       61 llrirnaapg wkcyldftft llnrehfskn ealsekqvrf saeepthgaa rwilisdlqt
      121 rrfsdetgef llelslanam tvfetdirvp pqllnhhhhh hhhrgsgagq qkdwaavgsa
      181 pgtptgsgga qtariessyf cfggfdwnia llpdgspedg dngrprvlli rhtgfdhpcr
      241 vqyrvvlgeg drrldsgvld qisdlsgrir gfslgaaapa dlvrrgtlri qlhmlsanai
      301 seakvpatat plagvepcvp atancydrek qawcveadle gerlrlklyy aelqhipvgh
      361 lryvawnayl vrrqpgsgar esvlalraph ssyyvhdgmd vavvmetdvs vrqiresagm
      421 yldgagnltv hvewlesqll fgatyhkydd iartqchqmk reiaalqaen ynlerqvfsy
      481 qksisyasar gqpsedlpdd yyastvrrls etqslsesey a
//
LOCUS       XP_077503516            1407 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113972 [Amblyomma americanum].
ACCESSION   XP_077503516
VERSION     XP_077503516.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647390.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1407
                     /product="uncharacterized protein LOC144113972"
                     /calculated_mol_wt=157767
     Region          436..996
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1407
                     /gene="LOC144113972"
                     /coded_by="XM_077647390.1:59..4282"
                     /db_xref="GeneID:144113972"
ORIGIN      
        1 mvdslaflvv wkeqskeidq yvsllrrfft tidiswparf hmsdvaqqlg thteeryyqa
       61 vctytfpdtg srgvpaselq tvlqtassrv tqqwcllgri mpfrghlqwl iperergfwr
      121 gwvphetdip laslsfgtfa glclfaqshv lysqagvpgf vlscafkhde rllqvmlmlv
      181 hndgcrhsss sykleipyhs ifrvvvneae tlhdavgiyl qlvtvpllyr gaddvnawea
      241 stedgvwydr vfqlgcpcgw lmhssdiggs svvkltlrnk lkarqaisrl sarckpettf
      301 qyspmltekv gaqlreikkr cdgkikpnlc fptcyalssv lqqghngfaq mallardkle
      361 efeeelirlr htsgpalela lyairsaiee heivdiiscl pklfakfctp atalpktpag
      421 tclvrrcivt pskvillppq ihcenrilrk fdpefslrvs frddnmqnls yslmsghcrh
      481 mavervvast lrdgliagnr qfrllatscs qlrdhgawly atdakgyssd mirywmgdfs
      541 giastakkma rmgqcfsste esvqvpllsn svhevpdilg gkhpvtdehy ifsdgigmis
      601 pellkevtdk iglpvvpsai qiryagykgm lcvnplitgr qlvlrksmrk fncvnsecie
      661 vikisaprpv flnrqlitll eqlgvpsrvf fclqqsmvtl ltealvsdsv alqvletyvg
      721 palpfsqlqr hgfsltrdpf vrsilfavyr tsmesllsks riavphnlgr nmfgvldetr
      781 tlkygqvfvq ftslgsptgh kngpefsils gtvlvtkcpc lhpgdvrkft avdvpalhri
      841 kdcvvfpang frphpdemag sdldgdeyiv iwdksllfpg pnkepmiygq kvqqqrsels
      901 lvdgmaqfis dyikndnvgv msnahlavad kledgvfsee clsiatkist cldfaktgva
      961 aalekherpq eypdfmekgc hkntyrsnrv lghlyrfqrf lesvvstsfs shlvdgrsni
     1021 kllefhgwvi ykcvveqlla ayesdmdril kqygikteae vvsgfindts sfnkshyeks
     1081 nvevlvtkqy raivestrer ffkkvdeach legalsvqer ktvllrmasa cfmvtysnah
     1141 ktcssfpwtf sdvillvmes aprsevpyrq gnlliaclds qlppaspqls aksialevvl
     1201 kwavkedlll qdagvrcari crlcletlfi ksagaedska ngkgapspgr yvtsffryvs
     1261 stsvvfpncd scswssqtht ltmaalrtys mlsifcdlch lglpcdarlh epvqeiqegn
     1321 svrikvtspg fqdmllhgqd evvsllavws gvhevrirrg vdrgggrhiv vssvgrdwqr
     1381 wfleelllqp wlataiekrs leqfirg
//
LOCUS       XP_077503517             673 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113973 [Amblyomma americanum].
ACCESSION   XP_077503517
VERSION     XP_077503517.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647391.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..673
                     /product="uncharacterized protein LOC144113973"
                     /calculated_mol_wt=73986
     Region          22..125
                     /region_name="HSF_DNA-bind"
                     /note="HSF-type DNA-binding; pfam00447"
                     /db_xref="CDD:459813"
     CDS             1..673
                     /gene="LOC144113973"
                     /coded_by="XM_077647391.1:104..2125"
                     /db_xref="GeneID:144113973"
ORIGIN      
        1 mhpyedykfq lskessmsfv rfpkklwkiv necnsgaisw spdgkavvie ydkfqkqyln
       61 gqremfktnn insfirqlnl ygfrkvsshy kvicanqhgg advhvfrnaa flrgrpells
      121 rvtrksglmr pkalqheddd dsanllprhn rrghgtdagv rgvsaqgrsl prrsqrrcnr
      181 avrppaatvn pltavaccsa gtdsdaittt neemtaamqr fmhgarrneg yipngdikal
      241 ldpdqaptvv lhhqgerprt plleesltln smnaylsdes tsdtlstalm ideclseqae
      301 fmhsaiqhia anqheaihqa avqhpvvqnv afphaavqea nwhpavlqav nwnpavpnap
      361 nrpmavhrps viqragvpqm paqypacqna afqhaveqaa fqnaanqhvp iqapteypav
      421 qlagfqniat qqagredgqh aaiqhapfeh idldytviee aatqqaateq aavqqvnlyp
      481 apqhmaiqhv alqhpaiqhq vdhhvafeqa ehapfqpvaf eyapfahmas ehaasqpatf
      541 epaasvpmpf epttfepavs qqastkhasf mctdainmai kkmaivntvi kneaivktai
      601 kttafqnagv qcvtvkkvai kkttveqval eqmtlergav hqtddqnmdk qqaifdhavd
      661 iatvapvaae aqc
//
LOCUS       XP_077503518             157 aa            linear   INV 25-NOV-2025
DEFINITION  MIP18 family protein galla-2 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503518
VERSION     XP_077503518.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647392.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..157
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..157
                     /product="MIP18 family protein galla-2 isoform X1"
                     /calculated_mol_wt=17350
     Region          5..156
                     /region_name="COG5133"
                     /note="Uncharacterized conserved protein [Function
                     unknown]; cl00941"
                     /db_xref="CDD:470001"
     CDS             1..157
                     /gene="galla-2"
                     /coded_by="XM_077647392.1:224..697"
                     /db_xref="GeneID:144113974"
ORIGIN      
        1 margdsldni apstyekakd rvttadelnd diedaidare ifdhirsind pehpltleel
       61 nvvdeknimv dldksaidva frptiphcsm atliglairv kllrslppaf kldvrilpgt
      121 hvseaainkq lddkervaaa lenshllqvv nrcllth
//
LOCUS       XP_077503519             154 aa            linear   INV 25-NOV-2025
DEFINITION  MIP18 family protein galla-2 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503519
VERSION     XP_077503519.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647393.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..154
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..154
                     /product="MIP18 family protein galla-2 isoform X2"
                     /calculated_mol_wt=16885
     Region          5..153
                     /region_name="COG5133"
                     /note="Uncharacterized conserved protein [Function
                     unknown]; cl00941"
                     /db_xref="CDD:470001"
     CDS             1..154
                     /gene="galla-2"
                     /coded_by="XM_077647393.1:225..689"
                     /db_xref="GeneID:144113974"
ORIGIN      
        1 margdsldni apstyekakd rvttadelnd diedaidare ifgsindpeh pltleelnvv
       61 deknimvdld ksaidvafrp tiphcsmatl iglairvkll rslppafkld vrilpgthvs
      121 eaainkqldd kervaaalen shllqvvnrc llth
//
LOCUS       XP_077503520             434 aa            linear   INV 25-NOV-2025
DEFINITION  tetratricopeptide repeat protein 5-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503520
VERSION     XP_077503520.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647394.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..434
                     /product="tetratricopeptide repeat protein 5-like isoform
                     X1"
                     /calculated_mol_wt=48429
     Region          77..311
                     /region_name="TPR"
                     /note="Tetratricopeptide (TPR) repeat [General function
                     prediction only]; COG0457"
                     /db_xref="CDD:440225"
     Region          79..108
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(80,83..84,87..88,90,115,118..119,122..123,125..126,
                     151..152,155..156,159)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          113..143
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          188..225
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          233..259
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          316..424
                     /region_name="TTC5_OB"
                     /note="Tetratricopeptide repeat protein 5 OB fold domain;
                     pfam16669"
                     /db_xref="CDD:465225"
     CDS             1..434
                     /gene="LOC144113975"
                     /coded_by="XM_077647394.1:17..1321"
                     /db_xref="GeneID:144113975"
ORIGIN      
        1 maddqaessk qrnenngdsf clkslkeavd elytfrdnyy vknpnasqkq rtedveeklt
       61 kvlneldahg gvlektdrat fqmlkgkaln iredydpeah ealskavkfn pklaeawnel
      121 gecywkkghi tkarncfegv lkvtkdkvsl rnlsmvlrqv geteeerlhn vlesvtkake
      181 alqldiadgk swsilgnayl tvyfsgaqnv kslqqalaay araledprml ccsdlfhnra
      241 valqheedyq caleqlerac slephwaqpr ekaqnliahl qrvsdaiahk ghlkprklka
      301 llsnlkaqpv aglvpaklqd lspgknsncv lsvkvacsvl sqerfpftmc vldeaaacla
      361 vnvynmaegr gfvtgdfvvi prpnlhhikv nhqgcelsfp sirvdtpldl ivngkalsse
      421 sraplrleve ikkd
//
LOCUS       XP_077503521             385 aa            linear   INV 25-NOV-2025
DEFINITION  tetratricopeptide repeat protein 5-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503521
VERSION     XP_077503521.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647395.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..385
                     /product="tetratricopeptide repeat protein 5-like isoform
                     X2"
                     /calculated_mol_wt=42771
     Region          77..>225
                     /region_name="TPR"
                     /note="Tetratricopeptide (TPR) repeat [General function
                     prediction only]; COG0457"
                     /db_xref="CDD:440225"
     Region          79..108
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(80,83..84,87..88,90,115,118..119,122..123,125..126,
                     151..152,155..156,159)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          113..143
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          188..225
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          267..375
                     /region_name="TTC5_OB"
                     /note="Tetratricopeptide repeat protein 5 OB fold domain;
                     pfam16669"
                     /db_xref="CDD:465225"
     CDS             1..385
                     /gene="LOC144113975"
                     /coded_by="XM_077647395.1:17..1174"
                     /db_xref="GeneID:144113975"
ORIGIN      
        1 maddqaessk qrnenngdsf clkslkeavd elytfrdnyy vknpnasqkq rtedveeklt
       61 kvlneldahg gvlektdrat fqmlkgkaln iredydpeah ealskavkfn pklaeawnel
      121 gecywkkghi tkarncfegv lkvtkdkvsl rnlsmvlrqv geteeerlhn vlesvtkake
      181 alqldiadgk swsilgnayl tvyfsgaqnv kslqqalaay araledprml ccsdlfhnra
      241 vghlkprklk allsnlkaqp vaglvpaklq dlspgknsnc vlsvkvacsv lsqerfpftm
      301 cvldeaaacl avnvynmaeg rgfvtgdfvv iprpnlhhik vnhqgcelsf psirvdtpld
      361 livngkalss esraplrlev eikkd
//
LOCUS       XP_077503522             379 aa            linear   INV 25-NOV-2025
DEFINITION  tetratricopeptide repeat protein 5-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503522
VERSION     XP_077503522.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647396.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..379
                     /product="tetratricopeptide repeat protein 5-like isoform
                     X3"
                     /calculated_mol_wt=43217
     Region          <5..>142
                     /region_name="3a0801s09"
                     /note="mitochondrial precursor proteins import receptor
                     (72 kDa mitochondrial outermembrane protein)
                     (mitochondrial import receptor for the ADP/ATP carrier)
                     (translocase of outermembrane tom70); TIGR00990"
                     /db_xref="CDD:273380"
     Region          77..288
                     /region_name="TPR"
                     /note="Tetratricopeptide (TPR) repeat [General function
                     prediction only]; COG0457"
                     /db_xref="CDD:440225"
     Region          79..108
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(80,83..84,87..88,90,115,118..119,122..123,125..126,
                     151..152,155..156,159)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          113..143
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          188..225
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          233..259
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     CDS             1..379
                     /gene="LOC144113975"
                     /coded_by="XM_077647396.1:17..1156"
                     /db_xref="GeneID:144113975"
ORIGIN      
        1 maddqaessk qrnenngdsf clkslkeavd elytfrdnyy vknpnasqkq rtedveeklt
       61 kvlneldahg gvlektdrat fqmlkgkaln iredydpeah ealskavkfn pklaeawnel
      121 gecywkkghi tkarncfegv lkvtkdkvsl rnlsmvlrqv geteeerlhn vlesvtkake
      181 alqldiadgk swsilgnayl tvyfsgaqnv kslqqalaay araledprml ccsdlfhnra
      241 valqheedyq caleqlerac slephwaqpr ekaqnliahl qrvsdaiahk vfqahcqphs
      301 hpwrhfmqer hcllkrlpsh vhdhmttsge tadsalpskq whdgcsyges rglmsqphlr
      361 rtscrrsncn trspwrnia
//
LOCUS       XP_077503523             352 aa            linear   INV 25-NOV-2025
DEFINITION  tetratricopeptide repeat protein 5-like isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503523
VERSION     XP_077503523.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647397.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..352
                     /product="tetratricopeptide repeat protein 5-like isoform
                     X4"
                     /calculated_mol_wt=39031
     Site            order(2,5..6,8,33,36..37,40..41,43..44,69..70,73..74,77)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          2..26
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          3..229
                     /region_name="TPR"
                     /note="Tetratricopeptide (TPR) repeat [General function
                     prediction only]; COG0457"
                     /db_xref="CDD:440225"
     Region          31..61
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          107..143
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          150..180
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          234..342
                     /region_name="TTC5_OB"
                     /note="Tetratricopeptide repeat protein 5 OB fold domain;
                     pfam16669"
                     /db_xref="CDD:465225"
     CDS             1..352
                     /gene="LOC144113975"
                     /coded_by="XM_077647397.1:1148..2206"
                     /db_xref="GeneID:144113975"
ORIGIN      
        1 mlkgkalnir edydpeahea lskavkfnpk laeawnelge cywkkghitk arncfegvlk
       61 vtkdkvslrn lsmvlrqvge teeerlhnvl esvtkakeal qldiadgksw silgnayltv
      121 yfsgaqnvks lqqalaayar aledprmlcc sdlfhnrava lqheedyqca leqleracsl
      181 ephwaqprek aqnliahlqr vsdaiahkgh lkprklkall snlkaqpvag lvpaklqdls
      241 pgknsncvls vkvacsvlsq erfpftmcvl deaaaclavn vynmaegrgf vtgdfvvipr
      301 pnlhhikvnh qgcelsfpsi rvdtpldliv ngkalssesr aplrleveik kd
//
LOCUS       XP_077503524             967 aa            linear   INV 25-NOV-2025
DEFINITION  cyclic nucleotide-gated ion channel subunit B [Amblyomma
            americanum].
ACCESSION   XP_077503524
VERSION     XP_077503524.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647398.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..967
                     /product="cyclic nucleotide-gated ion channel subunit B"
                     /calculated_mol_wt=107342
     Region          263..>649
                     /region_name="PLN03192"
                     /note="Voltage-dependent potassium channel; Provisional"
                     /db_xref="CDD:215625"
     CDS             1..967
                     /gene="CngB"
                     /coded_by="XM_077647398.1:95..2998"
                     /db_xref="GeneID:144113977"
ORIGIN      
        1 mldyilpdff aeekarmsle plvqegaqhg aichsrscsp ttgfqnpafr tevvrepckv
       61 savtrnavph qhqpagpiee daattarrrd ercccgeads dqrgppasga gspekpstrs
      121 ttpspstssa aaaaaaglsr raggadggrv qtristdltp lhtavisdse nttasefvse
      181 riqtlvrafs hrsqrvkeri aepptpspsp ppekredyes rlrldsfalv edapaesqae
      241 seqliriacc sfpkkaclfp rtidpqskly vswqlavsvc flyncwaifl ravflegtht
      301 deagffacdy lsdlvylldm llfkarikfh sagmwvedpk ltrrnyfrkf ifkmdvasll
      361 pldllyyvyg vnpllrlprm lkiqtfweff dridalaksp yvirvlrtll ymmylihlnt
      421 cayyaiskye gigsnpfvfq ggnktayvrc fyfafktats igknakpsne leyaymtlsw
      481 llgvflfafl igqvrdivat atqgrtqcrq avdacvrhlr rlglpddlqr rvrlwlnhtw
      541 aqkktldens ilatlprkmk tdiaisvhyn tlakvqffqd cersmlrdlv lklqsvlflp
      601 gdyicrkgei gvemyivnkg vinviggenn nvvlatlteg svfgevsllg lpgfnrrtad
      661 vlsvgfsnlf vlskddlndv lhdypdveam lrrkaksqmm knqasnektl eskarrihae
      721 piiktpparp ktpklvqtvl qvlrpesktg salarrmfgg ltsqrptsgs esdsssdsse
      781 amgrpttlsf rdedgvemrv dtyhdvtqna ltlratsasg lrvprsvppr cksspavmrh
      841 veerrserat pparrprpsp lrllgrclga dspesagrrh wrpltarsrv tpltppaphd
      901 laaegppade asatlrhsgg sapssraslr saslsgnels drdefrlvsp gfpsssspsw
      961 nfeeppc
//
LOCUS       XP_077503525             423 aa            linear   INV 25-NOV-2025
DEFINITION  malonyl-CoA-acyl carrier protein transacylase beg [Amblyomma
            americanum].
ACCESSION   XP_077503525
VERSION     XP_077503525.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647399.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..423
                     /product="malonyl-CoA-acyl carrier protein transacylase
                     beg"
                     /calculated_mol_wt=47007
     Region          107..414
                     /region_name="Acyl_transf_1"
                     /note="Acyl transferase domain; cl08282"
                     /db_xref="CDD:471802"
     CDS             1..423
                     /gene="beg"
                     /coded_by="XM_077647399.1:171..1442"
                     /db_xref="GeneID:144113978"
ORIGIN      
        1 mlpvvslver csrclvsksk stvlrraias tvlrhsqdnf andneetlsk asknadykkd
       61 nvarlfresa tfdaerqqnt dvwsspaypv sfkvpdeeky eetvridpkn tsvilfpgqg
      121 sqfvgmgrhl lqypnvaamy erasdilnyd lqllclegpk eqldktvhcq aavlvtslaa
      181 verlratqpw aiencvaaag fsvgeyaalv fsqaisfeda vrlvkirgea mqaaselvps
      241 glmtlflmtt ananfickla kewcarkdie dpvcsvanyl fphckviggn eealrfieln
      301 ardlgvkkmk rlpvsgafht almrparapl akalqavhik aprimthsnl dgiayrnpde
      361 irrkleeqlv mpvkweqlmq vvynrdvgee fprtfecgpg vtlravlknv ngkawnvses
      421 isa
//
LOCUS       XP_077503526             379 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial ribosomal protein L39 [Amblyomma americanum].
ACCESSION   XP_077503526
VERSION     XP_077503526.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647400.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..379
                     /product="mitochondrial ribosomal protein L39"
                     /calculated_mol_wt=42672
     Region          25..>308
                     /region_name="PLN02908"
                     /note="threonyl-tRNA synthetase"
                     /db_xref="CDD:178496"
     CDS             1..379
                     /gene="mRpL39"
                     /coded_by="XM_077647400.1:114..1253"
                     /db_xref="GeneID:144113979"
ORIGIN      
        1 msinssnrvl fllrgacsrh ryfcsssvat sesrltneav rkkrdalfta ekerqaslit
       61 rvekievqyl gpmedctlim nkgistphdc akhiheslvq raaiaevdge pwdmsrplvq
      121 dcqlrlnfyk dqnpyfanka fwrtgsfllg yiierafkdq fyvqlhswpq pqvrsgsfvy
      181 nvdlgienwe prrdeldvls gmgrrlatkp hhferlevda svalqmfedn kykaeqipri
      241 aatspsgntv tlyrlldhvd issgpmigsv dhmgrfkvvs vhamdtsvgl lyrfqglaip
      301 ssfsisefay tvcceraqrm nftglpqtes etgeeptedn vhfedmsaed ehvksettsn
      361 svtaatkdsa tatsqrtsa
//
LOCUS       XP_077503527            1138 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: Golgi apparatus protein 1 [Amblyomma
            americanum].
ACCESSION   XP_077503527
VERSION     XP_077503527.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647401.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 4 indels
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1138
                     /product="LOW QUALITY PROTEIN: Golgi apparatus protein 1"
                     /calculated_mol_wt=129718
     Region          121..178
                     /region_name="Cys_rich_FGFR"
                     /note="Cysteine rich repeat; pfam00839"
                     /db_xref="CDD:425899"
     Region          180..244
                     /region_name="Cys_rich_FGFR"
                     /note="Cysteine rich repeat; pfam00839"
                     /db_xref="CDD:425899"
     Region          245..298
                     /region_name="Cys_rich_FGFR"
                     /note="Cysteine rich repeat; pfam00839"
                     /db_xref="CDD:425899"
     Region          373..426
                     /region_name="Cys_rich_FGFR"
                     /note="Cysteine rich repeat; pfam00839"
                     /db_xref="CDD:425899"
     Region          437..494
                     /region_name="Cys_rich_FGFR"
                     /note="Cysteine rich repeat; pfam00839"
                     /db_xref="CDD:425899"
     Region          501..567
                     /region_name="Cys_rich_FGFR"
                     /note="Cysteine rich repeat; pfam00839"
                     /db_xref="CDD:425899"
     Region          574..630
                     /region_name="Cys_rich_FGFR"
                     /note="Cysteine rich repeat; pfam00839"
                     /db_xref="CDD:425899"
     Region          633..689
                     /region_name="Cys_rich_FGFR"
                     /note="Cysteine rich repeat; pfam00839"
                     /db_xref="CDD:425899"
     Region          697..746
                     /region_name="Cys_rich_FGFR"
                     /note="Cysteine rich repeat; pfam00839"
                     /db_xref="CDD:425899"
     Region          763..819
                     /region_name="Cys_rich_FGFR"
                     /note="Cysteine rich repeat; pfam00839"
                     /db_xref="CDD:425899"
     Region          821..875
                     /region_name="Cys_rich_FGFR"
                     /note="Cysteine rich repeat; pfam00839"
                     /db_xref="CDD:425899"
     Region          879..936
                     /region_name="Cys_rich_FGFR"
                     /note="Cysteine rich repeat; pfam00839"
                     /db_xref="CDD:425899"
     Region          948..997
                     /region_name="Cys_rich_FGFR"
                     /note="Cysteine rich repeat; pfam00839"
                     /db_xref="CDD:425899"
     Region          1003..1060
                     /region_name="Cys_rich_FGFR"
                     /note="Cysteine rich repeat; pfam00839"
                     /db_xref="CDD:425899"
     CDS             1..1138
                     /gene="Glg1"
                     /coded_by="XM_077647401.1:41..3457"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 2 bases in 2 codons;
                     deleted 2 bases in 2 codons"
                     /db_xref="GeneID:144113980"
ORIGIN      
        1 masrddrgsv tprctmscwr wnsswcclsa vlfafvllqq apfayclnaa aarnsqdnnv
       61 agnadskvrr vepptivkla ddptcaselk llcspsqltn nfnvlecvqn ahrkedegls
      121 eachhllwky rrditqdnrf ndaaskfcpr lmemfpqcvp gekgkllacl mdhsenatep
      181 echtflermq svvfsdyrlv ykftdacgpd idrftcgrip kssderhsqg atleclsrvi
      241 nqlkdncrre llhlarlqge dfhldrplff acqedrdrlc phvasgegri yrcllrhrss
      301 remseqcrek laqreqltmq dfrvsqglsg aclqdiriyr crektstrre frlaqillcl
      361 enaihkdypv gaecqqemle hrrflmenfq ltpdlassce qdiaafcrrr lepngktlhc
      421 lmrharpsvq gsqrlsdqcr rqvehvlkvs aagedwrvdp vlqeacqsta shlcqdvkpg
      481 rgrmlsclmd qvsniamkdt ckeallqiqy fvardfkldp vlykecrada vayckakkew
      541 yddptrmdpe rgpivlpcly ryayhpddsv rlskqclyei rrvmrqravs idlhpeieep
      601 cmsdlarmcs dhlgrgeemq clqdnlekls rxcrsavany teeeaehlel nyplyhscqa
      661 vlkdlcsdll skdvdqgdll gclvqhkndf rmkedxrcra alehfqlisl kdnkfsyafk
      721 eacrkdaqty cgnsksgadv vsclsklvld dvtdekvprs lhrcrlqlrv elfqreenik
      781 ldpkldaaca vdqrtlcsnv hpgegamlec lkdhknkltr echiavfqre rleaesvgld
      841 ysltlackpa lrlfcpevep sralrcladh rkeptmdvrc rnmvqrrlve qntdyrlnaq
      901 lqhacrmdia kfcsalvldk aaestelqgk viqclkaqfv rhqltktcep vvtgivrdaa
      961 ldyqldpvla racaseiqns ckddrdieec lksrfqnrei kspeckkeva rlihegkadv
     1021 qadpilykac lhdikhfchd ltpgqghlls clltglesdt ialteecrtl lskrvemfey
     1081 aaqvapvesi rdvvqqians psrnyfvvva mgvlgiifvg glfcgrvtkr lpanlknk
//
LOCUS       XP_077503528             329 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-tocopherol transfer protein-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503528
VERSION     XP_077503528.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647402.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..329
                     /product="alpha-tocopherol transfer protein-like isoform
                     X1"
                     /calculated_mol_wt=37570
     Region          62..108
                     /region_name="CRAL_TRIO_N"
                     /note="CRAL/TRIO, N-terminal domain; smart01100"
                     /db_xref="CDD:215024"
     Region          127..282
                     /region_name="SEC14"
                     /note="Sec14p-like lipid-binding domain; cd00170"
                     /db_xref="CDD:469559"
     CDS             1..329
                     /gene="LOC144113981"
                     /coded_by="XM_077647402.1:6..995"
                     /db_xref="GeneID:144113981"
ORIGIN      
        1 maglqtcqva cywtlswgak dtvktrtftl yvtntsmads alppqlrqia eeelgetdec
       61 rqqalhdlsk llndepglfw hnkpdivlrf lrvrkynvea alksitkyyr irsswgpifd
      121 nyrpssivpe crsimavlph rdaqgrhili vrngmwnptv vpqievhraf mlclgflers
      181 psgqtagivi indyggfgie qaiycklglm kdmldhvqes mplrlravhi vreskgfdfc
      241 falvrpflks kiiqrihihg hkleglyehv patilpeayg glvsdtifdk fwsqmdaqed
      301 lfveesrygy knsdshetps eleetithl
//
LOCUS       XP_077503529             293 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-tocopherol transfer protein-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503529
VERSION     XP_077503529.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647403.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..293
                     /product="alpha-tocopherol transfer protein-like isoform
                     X2"
                     /calculated_mol_wt=33542
     Region          26..72
                     /region_name="CRAL_TRIO_N"
                     /note="CRAL/TRIO, N-terminal domain; smart01100"
                     /db_xref="CDD:215024"
     Region          91..246
                     /region_name="SEC14"
                     /note="Sec14p-like lipid-binding domain; cd00170"
                     /db_xref="CDD:469559"
     CDS             1..293
                     /gene="LOC144113981"
                     /coded_by="XM_077647403.1:182..1063"
                     /db_xref="GeneID:144113981"
ORIGIN      
        1 madsalppql rqiaeeelge tdecrqqalh dlskllndep glfwhnkpdi vlrflrvrky
       61 nveaalksit kyyrirsswg pifdnyrpss ivpecrsima vlphrdaqgr hilivrngmw
      121 nptvvpqiev hrafmlclgf lerspsgqta giviindygg fgieqaiyck lglmkdmldh
      181 vqesmplrlr avhivreskg fdfcfalvrp flkskiiqri hihghklegl yehvpatilp
      241 eaygglvsdt ifdkfwsqmd aqedlfvees rygyknsdsh etpseleeti thl
//
LOCUS       XP_077503530             293 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-tocopherol transfer protein-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503530
VERSION     XP_077503530.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647404.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..293
                     /product="alpha-tocopherol transfer protein-like isoform
                     X2"
                     /calculated_mol_wt=33542
     Region          26..72
                     /region_name="CRAL_TRIO_N"
                     /note="CRAL/TRIO, N-terminal domain; smart01100"
                     /db_xref="CDD:215024"
     Region          91..246
                     /region_name="SEC14"
                     /note="Sec14p-like lipid-binding domain; cd00170"
                     /db_xref="CDD:469559"
     CDS             1..293
                     /gene="LOC144113981"
                     /coded_by="XM_077647404.1:136..1017"
                     /db_xref="GeneID:144113981"
ORIGIN      
        1 madsalppql rqiaeeelge tdecrqqalh dlskllndep glfwhnkpdi vlrflrvrky
       61 nveaalksit kyyrirsswg pifdnyrpss ivpecrsima vlphrdaqgr hilivrngmw
      121 nptvvpqiev hrafmlclgf lerspsgqta giviindygg fgieqaiyck lglmkdmldh
      181 vqesmplrlr avhivreskg fdfcfalvrp flkskiiqri hihghklegl yehvpatilp
      241 eaygglvsdt ifdkfwsqmd aqedlfvees rygyknsdsh etpseleeti thl
//
LOCUS       XP_077503531             293 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-tocopherol transfer protein-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503531
VERSION     XP_077503531.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647405.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..293
                     /product="alpha-tocopherol transfer protein-like isoform
                     X2"
                     /calculated_mol_wt=33542
     Region          26..72
                     /region_name="CRAL_TRIO_N"
                     /note="CRAL/TRIO, N-terminal domain; smart01100"
                     /db_xref="CDD:215024"
     Region          91..246
                     /region_name="SEC14"
                     /note="Sec14p-like lipid-binding domain; cd00170"
                     /db_xref="CDD:469559"
     CDS             1..293
                     /gene="LOC144113981"
                     /coded_by="XM_077647405.1:58..939"
                     /db_xref="GeneID:144113981"
ORIGIN      
        1 madsalppql rqiaeeelge tdecrqqalh dlskllndep glfwhnkpdi vlrflrvrky
       61 nveaalksit kyyrirsswg pifdnyrpss ivpecrsima vlphrdaqgr hilivrngmw
      121 nptvvpqiev hrafmlclgf lerspsgqta giviindygg fgieqaiyck lglmkdmldh
      181 vqesmplrlr avhivreskg fdfcfalvrp flkskiiqri hihghklegl yehvpatilp
      241 eaygglvsdt ifdkfwsqmd aqedlfvees rygyknsdsh etpseleeti thl
//
LOCUS       XP_077503532            1068 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier family 12 member kcc [Amblyomma americanum].
ACCESSION   XP_077503532
VERSION     XP_077503532.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647406.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1068
                     /product="solute carrier family 12 member kcc"
                     /calculated_mol_wt=117259
     Region          28..1068
                     /region_name="AA_permease_2"
                     /note="Amino acid permease; cl45918"
                     /db_xref="CDD:459263"
     CDS             1..1068
                     /gene="kcc"
                     /coded_by="XM_077647406.1:414..3620"
                     /db_xref="GeneID:144113982"
ORIGIN      
        1 mraqsaprfa fasaapsclc ctmlkanlgt lagvyfpciq nifgviffir lvwivgtagv
       61 pvafltvfmc ccvtfttcis lsaiatngiv paggsyfmis rslgpefgga vgilfylatt
      121 vaaamyitga veiflnylvp emslygdfre dqevmyhnfr tygsillvlm tfvvfigvaf
      181 vsklapialf cvlvsitsvy vgafvnyagk pgievcvlgd rilsggdync tkdrnvsnsl
      241 whifckenen ksleedhtcy pyfdeheahl rsalpglass affenipvmf kekgevvsss
      301 desyfpsksd syiqivcdit tsftflvaif fpsvtgimag snrsgdlada qksipvgtla
      361 aqmttsivyi sgvflfgaaf dnlflrdkfg esiggglava qlawphpllv vlgsllstig
      421 aglqsltgap rllqaiakdg vipvlnvfav sssrgepvra llltafisel gilignldhi
      481 apiltmfflm cymfvnlact lqsllktpnw rprfkyyhws lsltgvilcl vvmflsswyy
      541 alsamaiagi vykyieyrga ekewgdglrg lalsaarysl lrleegppht knwrpqvlvl
      601 cklnqdympk yrklitfasq lkagkgltlv csvlegeysk mysecqaskq slkkvleeer
      661 vkgfadvvsg gntidaichl iqtaglgglk hntvilgwpy gwrqspders wkvfietvrn
      721 vsasknallv pknisqfpdn teklhgnidv wwivhdggll mllpfllkqh kvwkncklri
      781 ftvaqledns iqmkkdlamf lyhlridadv evvemndsdi saytyertlm meqrtemlkh
      841 mrlsrretls miqtivdhhh nplqnhtssk vrfsdkppqq agdselvdva adednrssrh
      901 ssrhssptks asgsptkqas sppkesspvs aaedtekskt espekkaqdg sgdpgatsan
      961 gvaskgpsap taaaaatpdg qkkpefdsll klkpdeanvr rmhtavklne vivnkshdsk
     1021 lviinlpgpp riprgeenym eflevltegl ervlmvrggg revitiys
//
LOCUS       XP_077503533             421 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113983 [Amblyomma americanum].
ACCESSION   XP_077503533
VERSION     XP_077503533.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647407.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..421
                     /product="uncharacterized protein LOC144113983"
                     /calculated_mol_wt=47176
     Region          4..97
                     /region_name="THAP"
                     /note="The THAP domain is a putative DNA-binding domain
                     (DBD) and probably also binds a zinc ion; smart00980"
                     /db_xref="CDD:214951"
     Region          238..290
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          320..>421
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..421
                     /gene="LOC144113983"
                     /coded_by="XM_077647407.1:153..1418"
                     /db_xref="GeneID:144113983"
ORIGIN      
        1 mvlcaivgcs nrsdssrrkk kptntnfyri priiadkcer sktlstnrrt lwlsrikrad
       61 ldpdnpnfrv cgahfttgap adlfdntnpd wapslhlgys trpvgsdrhd rsqqrrqrkr
      121 caetemtgse epcgvavpvp dvppsphqae evdgageetg vyvqtdmgms digaleqeci
      181 rlndcvyttr sekvqlemte eslssepkvv fytgiasftt llaifkmlep fvshsvnnsl
      241 akfqefvlfl mkiklnlqna dlafrfnvse stvsrifdkw lhvahcrlks qvmwpersal
      301 qrtmpqafyd sfgsdvavii dcfeikierp ssylprsetw stykgsntak yligiapqgi
      361 vtfisegwgg rvsdkhiteh cglldnllpg dvvladrgfn vadsvgfyra rlhlpaytkg
      421 k
//
LOCUS       XP_077503534             294 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase RNF212B-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503534
VERSION     XP_077503534.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647408.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..294
                     /product="E3 ubiquitin-protein ligase RNF212B-like isoform
                     X1"
                     /calculated_mol_wt=32354
     Region          5..45
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(7,10,25,27,30,33,42,45)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          84..>131
                     /region_name="PRK03992"
                     /note="proteasome-activating nucleotidase; Provisional"
                     /db_xref="CDD:179699"
     Region          <116..139
                     /region_name="ZIP_TSC22D-like"
                     /note="leucine zipper found in the TSC22 domain leucine
                     zipper transcription factors, c-Myc-binding protein, and
                     similar proteins; cl40461"
                     /db_xref="CDD:424092"
     CDS             1..294
                     /gene="LOC144113986"
                     /coded_by="XM_077647408.1:208..1092"
                     /db_xref="GeneID:144113986"
ORIGIN      
        1 mnklvycnnc faqptrtslf fvttcahvfc qgckedctrs kckicnatcs taslsqnmsa
       61 dvrelfkdpk iimqrammis gfqlsqlsgl saylrqqvns lkvslkaatd neikykqene
      121 rlrkevaalk sevkelkdqq spginisesp tflaqpdypi ppafeqptaa ppvarmedfg
      181 qrtlgmqnfs ggaigytpsr rttammastd lsqqrghrty ahddqpasrm gvaqatlafg
      241 sstrerrslp gsdsslssir fwtqntdshs rysasilsta sidrrggmhs nlcl
//
LOCUS       XP_077503535             293 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase RNF212B-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503535
VERSION     XP_077503535.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647409.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..293
                     /product="E3 ubiquitin-protein ligase RNF212B-like isoform
                     X2"
                     /calculated_mol_wt=32298
     Region          5..45
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(7,10,25,27,30,33,42,45)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          84..>131
                     /region_name="PRK03992"
                     /note="proteasome-activating nucleotidase; Provisional"
                     /db_xref="CDD:179699"
     Region          <116..139
                     /region_name="ZIP_TSC22D-like"
                     /note="leucine zipper found in the TSC22 domain leucine
                     zipper transcription factors, c-Myc-binding protein, and
                     similar proteins; cl40461"
                     /db_xref="CDD:424092"
     CDS             1..293
                     /gene="LOC144113986"
                     /coded_by="XM_077647409.1:208..1089"
                     /db_xref="GeneID:144113986"
ORIGIN      
        1 mnklvycnnc faqptrtslf fvttcahvfc qgckedctrs kckicnatcs taslsqnmsa
       61 dvrelfkdpk iimqrammis gfqlsqlsgl saylrqqvns lkvslkaatd neikykqene
      121 rlrkevaalk sevkelkdqq spginisesp tflaqpdypi ppafeqptaa ppvarmedfg
      181 qrtlgmqnfs ggaigytpsr rttammastd lsqqrghrty ahddqpasrm gvaqatlafg
      241 sstrerrslp gsdsslssir fwtqntdshs rfpvsrcfse dhkssgkasp srs
//
LOCUS       XP_077503536             276 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase RNF212B-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503536
VERSION     XP_077503536.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647410.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..276
                     /product="E3 ubiquitin-protein ligase RNF212B-like isoform
                     X3"
                     /calculated_mol_wt=30589
     Region          5..45
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(7,10,25,27,30,33,42,45)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          84..>131
                     /region_name="PRK03992"
                     /note="proteasome-activating nucleotidase; Provisional"
                     /db_xref="CDD:179699"
     Region          <116..139
                     /region_name="ZIP_TSC22D-like"
                     /note="leucine zipper found in the TSC22 domain leucine
                     zipper transcription factors, c-Myc-binding protein, and
                     similar proteins; cl40461"
                     /db_xref="CDD:424092"
     CDS             1..276
                     /gene="LOC144113986"
                     /coded_by="XM_077647410.1:208..1038"
                     /db_xref="GeneID:144113986"
ORIGIN      
        1 mnklvycnnc faqptrtslf fvttcahvfc qgckedctrs kckicnatcs taslsqnmsa
       61 dvrelfkdpk iimqrammis gfqlsqlsgl saylrqqvns lkvslkaatd neikykqene
      121 rlrkevaalk sevkelkdqq spginisesp tflaqpdypi ppafeqptaa ppvarmedfg
      181 qrtlgmqnfs ggaigytpsr rttammastd lsqqrghrty ahddqpasrm gvaqatlafg
      241 sstrerrslp gsdsslssir fwtqntdshs rfedmf
//
LOCUS       XP_077503538             202 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113988 [Amblyomma americanum].
ACCESSION   XP_077503538
VERSION     XP_077503538.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647412.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..202
                     /product="uncharacterized protein LOC144113988"
                     /calculated_mol_wt=22411
     CDS             1..202
                     /gene="LOC144113988"
                     /coded_by="XM_077647412.1:1..609"
                     /db_xref="GeneID:144113988"
ORIGIN      
        1 mgiinvnspy cnlclgamts cpqicacyqa aifvllrvdh kklnyiiffs sllcsteddi
       61 sfklsadeag esstqavegl pealpqasgc dvegetwaqe cspdvyepea aqlppakrpr
      121 msqkqtaenp gnagdenilq acvshlstir eqrerqqkdd ialfcmllds rlrrlprqva
      181 edfmhniemm tydvmlqart ev
//
LOCUS       XP_077503539             536 aa            linear   INV 25-NOV-2025
DEFINITION  lipid scramblase CLPTM1L [Amblyomma americanum].
ACCESSION   XP_077503539
VERSION     XP_077503539.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647413.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..536
                     /product="lipid scramblase CLPTM1L"
                     /calculated_mol_wt=61971
     Region          6..421
                     /region_name="CLPTM1"
                     /note="Cleft lip and palate transmembrane protein 1
                     (CLPTM1); pfam05602"
                     /db_xref="CDD:461689"
     CDS             1..536
                     /gene="LOC144113991"
                     /coded_by="XM_077647413.1:215..1825"
                     /db_xref="GeneID:144113991"
ORIGIN      
        1 mkfswtvgls gvflayilhs mwtlytlyyp krcdknelci rptwstesrf qfffcittsk
       61 rvrrasdlav lwsedefdis ehqeknvnvt lprrtlrngt ldayvlller kkkepvrsle
      121 qvisisavst ssspltrylv ptgeevqlig alksdaqaqp sgmqesnpit hwnpkvevni
      181 msdpfpikan qipgeiahll qltsrreyvp ivhmtdvttr ekdfipvnks ipemplkivi
      241 spvsvgrlrl mvtvnealks mhafglttkd tdevksiffd tnfyvllmtl lvsgfhllfd
      301 flafkndvnf wrgrktfegl svkavlwrgf sqviiffyll dedtsrivla tsavgaiiev
      361 wkvtkafkis vhwsswrpwi efgsisaeet ktqeldseam rylsyllypl cawgavyslv
      421 yqsykswysw cihslangvy afgflfmlpq lflnyklksv ahlpwkvfmy kafntfiddv
      481 faflitmpta hrvacfrdda vflvylyqrw lypvdksrvn efgesfekpa ekprke
//
LOCUS       XP_077503540              94 aa            linear   INV 25-NOV-2025
DEFINITION  putative small nuclear ribonucleoprotein E [Amblyomma americanum].
ACCESSION   XP_077503540
VERSION     XP_077503540.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647414.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..94
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..94
                     /product="putative small nuclear ribonucleoprotein E"
                     /calculated_mol_wt=10811
     Region          12..90
                     /region_name="Sm_E"
                     /note="Sm protein E; cd01718"
                     /db_xref="CDD:212465"
     Site            order(25,37,39,42,49..52,56,64,73,75,77..81,84..90)
                     /site_type="other"
                     /note="heptamer interface [polypeptide binding]"
                     /db_xref="CDD:212465"
     Site            order(34..40,42..54,56..60)
                     /site_type="other"
                     /note="Sm1 motif"
                     /db_xref="CDD:212465"
     Site            order(52,54..56,81,83)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:212465"
     Site            77..88
                     /site_type="other"
                     /note="Sm2 motif"
                     /db_xref="CDD:212465"
     CDS             1..94
                     /gene="LOC144113992"
                     /coded_by="XM_077647414.1:151..435"
                     /db_xref="GeneID:144113992"
ORIGIN      
        1 mafkggpgqk vqkvmvqpin lifrylqnra riqvwlyenv alrieghiig fdeymnlvld
       61 daeeiytktk qrkqvgrill kgenitlims tqsm
//
LOCUS       XP_077503541             273 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113995 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503541
VERSION     XP_077503541.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647415.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..273
                     /product="uncharacterized protein LOC144113995 isoform X1"
                     /calculated_mol_wt=29429
     Region          75..150
                     /region_name="THAP"
                     /note="The THAP domain is a putative DNA-binding domain
                     (DBD) and probably also binds a zinc ion; smart00980"
                     /db_xref="CDD:214951"
     CDS             1..273
                     /gene="LOC144113995"
                     /coded_by="XM_077647415.1:857..1678"
                     /db_xref="GeneID:144113995"
ORIGIN      
        1 mlllrsfrrp ctsllchrsv rfaprctaaa vfsilsfpsa cgqcevffrl ctlralllkr
       61 lalhfkmslr hvkkrcsvvh cdsvdstigv tlhrfpldfh rcrawaqfcr nvslekkaps
      121 qlrelricsr hfepsvylps grlrhdalpt rasiadtsse cssdmdcsqs lsetsasvrr
      181 agepaavsge pvskgyvqsr ssqslllfqe dadgasptgs mlnqacshas gfimvqsdht
      241 yagpsteasa sslepgtagv aatlsasadl spg
//
LOCUS       XP_077503542             109 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113995 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503542
VERSION     XP_077503542.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647416.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..109
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..109
                     /product="uncharacterized protein LOC144113995 isoform X2"
                     /calculated_mol_wt=10725
     CDS             1..109
                     /gene="LOC144113995"
                     /coded_by="XM_077647416.1:1107..1436"
                     /db_xref="GeneID:144113995"
ORIGIN      
        1 mdcsqslset sasvrragep aavsgepvsk gyvqsrssqs lllfqedadg asptgsmlnq
       61 acshasgfim vqsdhtyagp steasassle pgtagvaatl sasadlspg
//
LOCUS       XP_077503543             828 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113996 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503543
VERSION     XP_077503543.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647417.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..828
                     /product="uncharacterized protein LOC144113996 isoform X1"
                     /calculated_mol_wt=94888
     Region          <70..758
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..828
                     /gene="LOC144113996"
                     /coded_by="XM_077647417.1:289..2775"
                     /db_xref="GeneID:144113996"
ORIGIN      
        1 msfpraprft dvvedtppvg synpllrart papkfstndr fkpgsdlsll svpcsspave
       61 sfkvpqtpsr lqrdklqrrl remtghleer daqisklras rehlisriky lektlqsvth
      121 kvsarresis vkklqcreas sgayenlaah eatemlaevk lskgtaelke eiekfindfr
      181 dddannfsig sdsddlarek dlehafekly seneklvitv gelqcevkal knhhedlegk
      241 haklieqavn heneksklqk kfneimteke almenvkrlq aencrlecay ssialrceei
      301 esqldevsce daclrsllhd rgneilvleq hkqdlleklg efesvistmv daeqelssrl
      361 sdleslhsrn kelssmkmnl eqelkmvkda lhaledekrm terdltklle dksvlqesnq
      421 sylakhaqle kelfktkhel evcqseakhl sgencrltke lsavisshea tvlknrelek
      481 sldgvskekn tmeehlesvt dalktveasl nqvnaehlkl rhekacleee vcvirgrnee
      541 lnasktslqm qlsgalaqls aaerckqqlq eelalteeak heaikdalhk dkalkslesq
      601 nnnlakalle qqklcsklem elvslqdaad llqarneelq fstdasklqf svaerranky
      661 klvcseldlq krdlcdqikq lseeltelrd gqvaglteql kdktveaekw kfnfealqlr
      721 vapfqgqlel yemekeller rneateselt klhrkvsemm ghqnhrqkih ylsnllkern
      781 eykkevytlh ekmlkqmkei kklevllqqr grksvlelst kenldppa
//
LOCUS       XP_077503544             822 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113996 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503544
VERSION     XP_077503544.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647418.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..822
                     /product="uncharacterized protein LOC144113996 isoform X2"
                     /calculated_mol_wt=94259
     Region          <64..752
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..822
                     /gene="LOC144113996"
                     /coded_by="XM_077647418.1:199..2667"
                     /db_xref="GeneID:144113996"
ORIGIN      
        1 msfpraprft dvvedtppvg synpllrart papkfstndr fkpgsvpcss pavesfkvpq
       61 tpsrlqrdkl qrrlremtgh leerdaqisk lrasrehlis rikylektlq svthkvsarr
      121 esisvkklqc reassgayen laaheateml aevklskgta elkeeiekfi ndfrdddann
      181 fsigsdsddl arekdlehaf eklysenekl vitvgelqce vkalknhhed legkhaklie
      241 qavnheneks klqkkfneim tekealmenv krlqaencrl ecayssialr ceeiesqlde
      301 vscedaclrs llhdrgneil vleqhkqdll eklgefesvi stmvdaeqel ssrlsdlesl
      361 hsrnkelssm kmnleqelkm vkdalhaled ekrmterdlt klledksvlq esnqsylakh
      421 aqlekelfkt khelevcqse akhlsgencr ltkelsavis sheatvlknr eleksldgvs
      481 kekntmeehl esvtdalktv easlnqvnae hlklrhekac leeevcvirg rneelnaskt
      541 slqmqlsgal aqlsaaerck qqlqeelalt eeakheaikd alhkdkalks lesqnnnlak
      601 alleqqklcs klemelvslq daadllqarn eelqfstdas klqfsvaerr ankyklvcse
      661 ldlqkrdlcd qikqlseelt elrdgqvagl teqlkdktve aekwkfnfea lqlrvapfqg
      721 qlelyemeke llerrneate seltklhrkv semmghqnhr qkihylsnll kerneykkev
      781 ytlhekmlkq mkeikklevl lqqrgrksvl elstkenldp pa
//
LOCUS       XP_077503545             246 aa            linear   INV 25-NOV-2025
DEFINITION  repressor of RNA polymerase III transcription Maf1 [Amblyomma
            americanum].
ACCESSION   XP_077503545
VERSION     XP_077503545.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647419.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..246
                     /product="repressor of RNA polymerase III transcription
                     Maf1"
                     /calculated_mol_wt=27442
     Region          25..207
                     /region_name="Maf1"
                     /note="Maf1 regulator; pfam09174"
                     /db_xref="CDD:462704"
     CDS             1..246
                     /gene="Maf1"
                     /coded_by="XM_077647419.1:761..1501"
                     /db_xref="GeneID:144113997"
ORIGIN      
        1 mkllessrfe ainsslcfet gdckivgrie sysckmvgsd krlfktmnae agggpndlqa
       61 lsppqsalms qspsklysrs qsddgechlc dtisrktlfh ligtlnasfh pdydfsqaks
      121 defsretsle wvmntvdsnl fatanqaysa lrgrlwaavd seisladcdi fsynpdlasd
      181 pygedgclws fnfffynrrl krivfftcha vsansseygc deleqmnmel eeesvanpnr
      241 aiqeaf
//
LOCUS       XP_077503546             595 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-1,2-mannosyltransferase Alg9 [Amblyomma americanum].
ACCESSION   XP_077503546
VERSION     XP_077503546.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647420.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..595
                     /product="alpha-1,2-mannosyltransferase Alg9"
                     /calculated_mol_wt=66876
     Region          42..471
                     /region_name="Glyco_transf_22"
                     /note="Alg9-like mannosyltransferase family; pfam03901"
                     /db_xref="CDD:281842"
     CDS             1..595
                     /gene="Alg9"
                     /coded_by="XM_077647420.1:153..1940"
                     /db_xref="GeneID:144113998"
ORIGIN      
        1 marqrrkepr dhprklaaka rpaasstcdd qhvgpwapvp ytafkallsa rlcaavwnni
       61 adcdetfnyw epthylmygk glqtweyspk yairsytylw lhalpglvys sllqsnrvlv
      121 fyfmrcilgf vcalcevyfy kavlrrfgpv varstlvlll fstgmfisct aylpssfsmy
      181 stmvafggwf lgdhrvtvfa tavgtllgwp fsavlslpaa ydivcvrrkl vpflvscvas
      241 lvvvlpvlvt idthyfgrtv laplnivmyn vfskhganly gvepwtfyik ngmlnfnvaf
      301 aaalasapav llsrklkqpv spsqgragad cllelapfyl wcaiffsqah keerflfpvy
      361 pliclcaaic graletlaah ysrsltgvar raasavaqhl wlvllvattl lsvsrtvaly
      421 rnfrapmevy melgplanla adghdnqedi vpstlcvgke wyrfpssffl pqnwelafie
      481 sefrgqlpkp yasganatri iptdmndanr eeqsryvnls lcdylvdtdg hdvtdrepey
      541 ssspewevvs svkfldsrrs pvyarafyvp fvserlcsyv nyyllkrkkt tknna
//
LOCUS       XP_077503547             211 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144113999 [Amblyomma americanum].
ACCESSION   XP_077503547
VERSION     XP_077503547.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647421.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..211
                     /product="uncharacterized protein LOC144113999"
                     /calculated_mol_wt=22967
     Region          31..115
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..211
                     /gene="LOC144113999"
                     /coded_by="XM_077647421.1:125..760"
                     /db_xref="GeneID:144113999"
ORIGIN      
        1 mvsiflcrll lsgvpeplav sssppemntp ccvvgckpad wrgvelrhvi prgvreraaw
       61 meriglpash arrrltvcgr hsapdayyyn palvrlsgmn vkrlklrpkp lptlflptgk
      121 epahtctcsa arltvatqtr keslcasert recqsvatqv slglkvlvsa gtqtdpcalq
      181 eapvekfvck qgnthteall llptrfrsrg a
//
LOCUS       XP_077503548             142 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114000 [Amblyomma americanum].
ACCESSION   XP_077503548
VERSION     XP_077503548.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647422.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..142
                     /product="uncharacterized protein LOC144114000"
                     /calculated_mol_wt=15904
     Region          <21..138
                     /region_name="P2RX7_C"
                     /note="P2X purinoreceptor 7 intracellular domain;
                     pfam20478"
                     /db_xref="CDD:466627"
     CDS             1..142
                     /gene="LOC144114000"
                     /coded_by="XM_077647422.1:668..1096"
                     /db_xref="GeneID:144114000"
ORIGIN      
        1 mrsrhtgwds pgsggddnip wcscghceet ttleerlccc gvpweiseqe dgcitlhpvf
       61 dslccapqvl qavfleflng vdgerdddln rqyryvayrl ftrwiwgtlg ignrvvlpac
      121 vvarirrqsp sasyvgfrhp pl
//
LOCUS       XP_077503549             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077503549
VERSION     XP_077503549.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647423.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=49564
     Region          1..430
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144114001"
                     /coded_by="XM_077647423.1:54..1385"
                     /db_xref="GeneID:144114001"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gtidavrsgp fgplfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrdcqq yraltvpelt qemfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkdssy fvewipnnvk tavcdipprg
      361 lkmsatfign staiqelfkr iseqftamfr rkaflhwytg egmdelefte aesnlnelvs
      421 eyqqyqeatp edeeefeetq aea
//
LOCUS       XP_077503550             285 aa            linear   INV 25-NOV-2025
DEFINITION  anamorsin homolog isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503550
VERSION     XP_077503550.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647424.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..285
                     /product="anamorsin homolog isoform X1"
                     /calculated_mol_wt=30879
     Region          206..266
                     /region_name="CIAPIN1"
                     /note="Cytokine-induced anti-apoptosis inhibitor 1, Fe-S
                     biogenesis; pfam05093"
                     /db_xref="CDD:398662"
     CDS             1..285
                     /gene="LOC144114002"
                     /coded_by="XM_077647424.1:138..995"
                     /db_xref="GeneID:144114002"
ORIGIN      
        1 mdqvlkrgnr vlvlwegqvv aerlkdvvek iqsliqdpsl cqfenaqklp eskhpgssfd
       61 atvlgfvtpq mrpssdtlvn iinllkpngk llvklsgadl dsgnlvynlk lsgfvdicqn
      121 tsdgdsveit cskpsyeigs ssklpfaeat vgsqepsadv gkiwslsaqd mldgdvelvd
      181 pdqliteedf qkpdpsalka acgsekkrra crnctcglae eldreaaavq qpksscgncy
      241 lgdafrcasc pyrglpafkp gekivltedq lavdnsqrlk kielr
//
LOCUS       XP_077503551             285 aa            linear   INV 25-NOV-2025
DEFINITION  anamorsin homolog isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503551
VERSION     XP_077503551.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647425.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..285
                     /product="anamorsin homolog isoform X1"
                     /calculated_mol_wt=30879
     Region          206..266
                     /region_name="CIAPIN1"
                     /note="Cytokine-induced anti-apoptosis inhibitor 1, Fe-S
                     biogenesis; pfam05093"
                     /db_xref="CDD:398662"
     CDS             1..285
                     /gene="LOC144114002"
                     /coded_by="XM_077647425.1:131..988"
                     /db_xref="GeneID:144114002"
ORIGIN      
        1 mdqvlkrgnr vlvlwegqvv aerlkdvvek iqsliqdpsl cqfenaqklp eskhpgssfd
       61 atvlgfvtpq mrpssdtlvn iinllkpngk llvklsgadl dsgnlvynlk lsgfvdicqn
      121 tsdgdsveit cskpsyeigs ssklpfaeat vgsqepsadv gkiwslsaqd mldgdvelvd
      181 pdqliteedf qkpdpsalka acgsekkrra crnctcglae eldreaaavq qpksscgncy
      241 lgdafrcasc pyrglpafkp gekivltedq lavdnsqrlk kielr
//
LOCUS       XP_077503552             285 aa            linear   INV 25-NOV-2025
DEFINITION  anamorsin homolog isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503552
VERSION     XP_077503552.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647426.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..285
                     /product="anamorsin homolog isoform X1"
                     /calculated_mol_wt=30879
     Region          206..266
                     /region_name="CIAPIN1"
                     /note="Cytokine-induced anti-apoptosis inhibitor 1, Fe-S
                     biogenesis; pfam05093"
                     /db_xref="CDD:398662"
     CDS             1..285
                     /gene="LOC144114002"
                     /coded_by="XM_077647426.1:219..1076"
                     /db_xref="GeneID:144114002"
ORIGIN      
        1 mdqvlkrgnr vlvlwegqvv aerlkdvvek iqsliqdpsl cqfenaqklp eskhpgssfd
       61 atvlgfvtpq mrpssdtlvn iinllkpngk llvklsgadl dsgnlvynlk lsgfvdicqn
      121 tsdgdsveit cskpsyeigs ssklpfaeat vgsqepsadv gkiwslsaqd mldgdvelvd
      181 pdqliteedf qkpdpsalka acgsekkrra crnctcglae eldreaaavq qpksscgncy
      241 lgdafrcasc pyrglpafkp gekivltedq lavdnsqrlk kielr
//
LOCUS       XP_077503553             284 aa            linear   INV 25-NOV-2025
DEFINITION  anamorsin homolog isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503553
VERSION     XP_077503553.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647427.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..284
                     /product="anamorsin homolog isoform X2"
                     /calculated_mol_wt=30808
     Region          205..265
                     /region_name="CIAPIN1"
                     /note="Cytokine-induced anti-apoptosis inhibitor 1, Fe-S
                     biogenesis; pfam05093"
                     /db_xref="CDD:398662"
     CDS             1..284
                     /gene="LOC144114002"
                     /coded_by="XM_077647427.1:138..992"
                     /db_xref="GeneID:144114002"
ORIGIN      
        1 mdqvlkrgnr vlvlwegqvv aerlkdvvek iqsliqdpsl cqfenaqklp eskhpgssfd
       61 atvlgfvtpq mrpssdtlvn iinllkpngk llvklsgadl dsgnlvynlk lsgfvdicqn
      121 tsdgdsveit cskpsyeigs ssklpfaeat vgsqepsadv gkiwslsaqd mldgdvelvd
      181 pdqliteedf qkpdpsalka cgsekkrrac rnctcglaee ldreaaavqq pksscgncyl
      241 gdafrcascp yrglpafkpg ekivltedql avdnsqrlkk ielr
//
LOCUS       XP_077503554             284 aa            linear   INV 25-NOV-2025
DEFINITION  anamorsin homolog isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503554
VERSION     XP_077503554.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647428.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..284
                     /product="anamorsin homolog isoform X2"
                     /calculated_mol_wt=30808
     Region          205..265
                     /region_name="CIAPIN1"
                     /note="Cytokine-induced anti-apoptosis inhibitor 1, Fe-S
                     biogenesis; pfam05093"
                     /db_xref="CDD:398662"
     CDS             1..284
                     /gene="LOC144114002"
                     /coded_by="XM_077647428.1:131..985"
                     /db_xref="GeneID:144114002"
ORIGIN      
        1 mdqvlkrgnr vlvlwegqvv aerlkdvvek iqsliqdpsl cqfenaqklp eskhpgssfd
       61 atvlgfvtpq mrpssdtlvn iinllkpngk llvklsgadl dsgnlvynlk lsgfvdicqn
      121 tsdgdsveit cskpsyeigs ssklpfaeat vgsqepsadv gkiwslsaqd mldgdvelvd
      181 pdqliteedf qkpdpsalka cgsekkrrac rnctcglaee ldreaaavqq pksscgncyl
      241 gdafrcascp yrglpafkpg ekivltedql avdnsqrlkk ielr
//
LOCUS       XP_077503555             297 aa            linear   INV 25-NOV-2025
DEFINITION  epimerase family protein SDR39U1 [Amblyomma americanum].
ACCESSION   XP_077503555
VERSION     XP_077503555.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647429.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..297
                     /product="epimerase family protein SDR39U1"
                     /calculated_mol_wt=32435
     Region          16..294
                     /region_name="NADB_Rossmann"
                     /note="Rossmann-fold NAD(P)(+)-binding proteins; cl21454"
                     /db_xref="CDD:473865"
     Site            order(83,109,133,137)
                     /site_type="active"
                     /db_xref="CDD:187535"
     CDS             1..297
                     /gene="LOC144114004"
                     /coded_by="XM_077647429.1:75..968"
                     /db_xref="GeneID:144114004"
ORIGIN      
        1 mekvvigggt gfigtalrka lnklgydvlv vsrkpganvl twtdlaekgl pkctavfsla
       61 gqnvmdpmrr wtpgfrqnvy asrvettkal adaikkmesp pkafvsisgv gyykpdpvee
      121 yteespggdh dflaklctkw eaaaklppev kcrtvtvrsg vvlgsnggmv qqlywpfffg
      181 lggpvasgtq fmpwvhisdi vgilihamdk dgltgvlngv apqvvtnaef tkefaramwr
      241 palfplpkfv ldlafspera tmmtegqkvi pkrtlesgyk ykfpdvrsac qdvlqrk
//
LOCUS       XP_077503557             522 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114005 [Amblyomma americanum].
ACCESSION   XP_077503557
VERSION     XP_077503557.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647431.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..522
                     /product="uncharacterized protein LOC144114005"
                     /calculated_mol_wt=59181
     CDS             1..522
                     /gene="LOC144114005"
                     /coded_by="XM_077647431.1:258..1826"
                     /db_xref="GeneID:144114005"
ORIGIN      
        1 mwlllmrsct sqddfrmkrg lvsscidlfv sfgkiaamel eqrisnwlpa ldkeiveltv
       61 qklqqcgves lehlkyvvee dlqflkpisr rillerfhsa atpnppvsls pndiqsptst
      121 qcasfstpwe vfppqlmkac qegkrpvksd lnemvrlvsd hilainrkpg rkilraiaae
      181 ivshypktfe dtlngavigs gietllwkle ncvnnkrrps seqphqfepd deldlsvkrv
      241 kiqrdsygcv awqpklpsde sadsqekkke dllsqfrlaf pdetmvaqlm setyasqrql
      301 inasknirdi erswpflfqa khltnhvnil lgsnvaktfd drlktvgrqv fryahsqvkk
      361 ecvksclqei eaaktnrksm aveyeampll llaifgedke lfyklteema sdddlgdlps
      421 cpfictkgwt fltansytic vdthpvlhas kpdeafkllf fahfafniqy qketslclef
      481 tqraiaginp argtkvrkng gkqhclsprv aalatalkdy df
//
LOCUS       XP_077503558             659 aa            linear   INV 25-NOV-2025
DEFINITION  GTP-Rho-binding protein rhophilin isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503558
VERSION     XP_077503558.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647432.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..659
                     /product="GTP-Rho-binding protein rhophilin isoform X1"
                     /calculated_mol_wt=74561
     Region          18..102
                     /region_name="HR1_Rhophilin-1"
                     /note="Protein kinase C-related kinase homology region 1
                     (HR1) Rho-binding domain of Rhophilin-1; cd11633"
                     /db_xref="CDD:212023"
     Site            order(35,38,41..42,45..46,48..49,52..53,55..56,71..72,75,
                     78..79,82)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212023"
     Site            order(43..44,46..47,50..51,53..54,57..58,66..67,69)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212023"
     Region          106..490
                     /region_name="BRO1_Rhophilin"
                     /note="Protein-interacting Bro1-like domain of
                     RhoA-binding protein Rhophilin and related domains;
                     cd09244"
                     /db_xref="CDD:185767"
     Site            order(221,224,237,240..241,285,288..289,301,304..305,474,
                     476,478,480)
                     /site_type="other"
                     /note="putative ESCRT-III binding site"
                     /db_xref="CDD:185767"
     Region          509..585
                     /region_name="PDZ_rhophilin-like"
                     /note="PDZ domain of rhophilin-1, rhophilin-2, and related
                     domains; cd06712"
                     /db_xref="CDD:467196"
     Site            order(520..526,534,537,566,570,573..574)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467196"
     CDS             1..659
                     /gene="Rhp"
                     /coded_by="XM_077647432.1:1049..3028"
                     /db_xref="GeneID:144114006"
ORIGIN      
        1 metwienlnn lvialkqgsd praatcrgkl qyrrskinhe itkqmmmrng aeklfkattn
       61 kklretvale lsflnsnlql lkeqlaelns svqiyqsenq sqvvpmiplg lketkdidfk
      121 epfkdfimeh ysedssvyds aiqefmdvrq aartptrdqa gvqllfeyyn llyfvdrrff
      181 ppnrsmgiff ewfdsltgvp stqktvafek asvlfniaal ytqigakqdr gkstgidaav
      241 dsflraagmf cyirenfskp psmdlsaetl evlthvmlaq areclfekli lgnvekqgvm
      301 tcievgqeaa evaevyaqvh klismppvkd yvpyswisli lvkaehyral ahyyvavgll
      361 ehsgdldeal rrsleflhvq eqsnstcidi rvpqtmeerr qlgkahlrea lllheealrl
      421 hrmcrqlrqv dnllgrlrad hdaawqrysd leeeddfqev ldppqiqast kyqltltspd
      481 ftqykvkdlf hglgpisifs arhhwsasra vhltkkgkes fgfsvrgdsp vivagveacs
      541 laelagvkeg dfivaigdld tkwstheevv qlirkagdel tlrvitpmdr sylkqspass
      601 ttsssgvsss gssrvqspng siasnkskds hkrltwnffr rgtskerrea gydanilmr
//
LOCUS       XP_077503559             655 aa            linear   INV 25-NOV-2025
DEFINITION  GTP-Rho-binding protein rhophilin isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503559
VERSION     XP_077503559.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647433.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..655
                     /product="GTP-Rho-binding protein rhophilin isoform X2"
                     /calculated_mol_wt=74172
     Region          14..98
                     /region_name="HR1_Rhophilin-1"
                     /note="Protein kinase C-related kinase homology region 1
                     (HR1) Rho-binding domain of Rhophilin-1; cd11633"
                     /db_xref="CDD:212023"
     Site            order(31,34,37..38,41..42,44..45,48..49,51..52,67..68,71,
                     74..75,78)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212023"
     Site            order(39..40,42..43,46..47,49..50,53..54,62..63,65)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212023"
     Region          102..486
                     /region_name="BRO1_Rhophilin"
                     /note="Protein-interacting Bro1-like domain of
                     RhoA-binding protein Rhophilin and related domains;
                     cd09244"
                     /db_xref="CDD:185767"
     Site            order(217,220,233,236..237,281,284..285,297,300..301,470,
                     472,474,476)
                     /site_type="other"
                     /note="putative ESCRT-III binding site"
                     /db_xref="CDD:185767"
     Region          505..581
                     /region_name="PDZ_rhophilin-like"
                     /note="PDZ domain of rhophilin-1, rhophilin-2, and related
                     domains; cd06712"
                     /db_xref="CDD:467196"
     Site            order(516..522,530,533,562,566,569..570)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467196"
     CDS             1..655
                     /gene="Rhp"
                     /coded_by="XM_077647433.1:436..2403"
                     /db_xref="GeneID:144114006"
ORIGIN      
        1 masslrypqf rkrgsdpraa tcrgklqyrr skinheitkq mmmrngaekl fkattnkklr
       61 etvalelsfl nsnlqllkeq laelnssvqi yqsenqsqvv pmiplglket kdidfkepfk
      121 dfimehysed ssvydsaiqe fmdvrqaart ptrdqagvql lfeyynllyf vdrrffppnr
      181 smgiffewfd sltgvpstqk tvafekasvl fniaalytqi gakqdrgkst gidaavdsfl
      241 raagmfcyir enfskppsmd lsaetlevlt hvmlaqarec lfeklilgnv ekqgvmtcie
      301 vgqeaaevae vyaqvhklis mppvkdyvpy swislilvka ehyralahyy vavgllehsg
      361 dldealrrsl eflhvqeqsn stcidirvpq tmeerrqlgk ahlrealllh eealrlhrmc
      421 rqlrqvdnll grlradhdaa wqrysdleee ddfqevldpp qiqastkyql tltspdftqy
      481 kvkdlfhglg pisifsarhh wsasravhlt kkgkesfgfs vrgdspviva gveacslael
      541 agvkegdfiv aigdldtkws theevvqlir kagdeltlrv itpmdrsylk qspassttss
      601 sgvsssgssr vqspngsias nkskdshkrl twnffrrgts kerreagyda nilmr
//
LOCUS       XP_077503560             646 aa            linear   INV 25-NOV-2025
DEFINITION  GTP-Rho-binding protein rhophilin isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503560
VERSION     XP_077503560.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647434.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..646
                     /product="GTP-Rho-binding protein rhophilin isoform X3"
                     /calculated_mol_wt=72996
     Region          5..89
                     /region_name="HR1_Rhophilin-1"
                     /note="Protein kinase C-related kinase homology region 1
                     (HR1) Rho-binding domain of Rhophilin-1; cd11633"
                     /db_xref="CDD:212023"
     Site            order(22,25,28..29,32..33,35..36,39..40,42..43,58..59,62,
                     65..66,69)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212023"
     Site            order(30..31,33..34,37..38,40..41,44..45,53..54,56)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212023"
     Region          93..477
                     /region_name="BRO1_Rhophilin"
                     /note="Protein-interacting Bro1-like domain of
                     RhoA-binding protein Rhophilin and related domains;
                     cd09244"
                     /db_xref="CDD:185767"
     Site            order(208,211,224,227..228,272,275..276,288,291..292,461,
                     463,465,467)
                     /site_type="other"
                     /note="putative ESCRT-III binding site"
                     /db_xref="CDD:185767"
     Region          496..572
                     /region_name="PDZ_rhophilin-like"
                     /note="PDZ domain of rhophilin-1, rhophilin-2, and related
                     domains; cd06712"
                     /db_xref="CDD:467196"
     Site            order(507..513,521,524,553,557,560..561)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467196"
     CDS             1..646
                     /gene="Rhp"
                     /coded_by="XM_077647434.1:69..2009"
                     /db_xref="GeneID:144114006"
ORIGIN      
        1 maedgsdpra atcrgklqyr rskinheitk qmmmrngaek lfkattnkkl retvalelsf
       61 lnsnlqllke qlaelnssvq iyqsenqsqv vpmiplglke tkdidfkepf kdfimehyse
      121 dssvydsaiq efmdvrqaar tptrdqagvq llfeyynlly fvdrrffppn rsmgiffewf
      181 dsltgvpstq ktvafekasv lfniaalytq igakqdrgks tgidaavdsf lraagmfcyi
      241 renfskppsm dlsaetlevl thvmlaqare clfeklilgn vekqgvmtci evgqeaaeva
      301 evyaqvhkli smppvkdyvp yswislilvk aehyralahy yvavgllehs gdldealrrs
      361 leflhvqeqs nstcidirvp qtmeerrqlg kahlrealll heealrlhrm crqlrqvdnl
      421 lgrlradhda awqrysdlee eddfqevldp pqiqastkyq ltltspdftq ykvkdlfhgl
      481 gpisifsarh hwsasravhl tkkgkesfgf svrgdspviv agveacslae lagvkegdfi
      541 vaigdldtkw stheevvqli rkagdeltlr vitpmdrsyl kqspasstts ssgvsssgss
      601 rvqspngsia snkskdshkr ltwnffrrgt skerreagyd anilmr
//
LOCUS       XP_077503561            1486 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin carboxyl-terminal hydrolase CYLD isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503561
VERSION     XP_077503561.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647435.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1486
                     /product="ubiquitin carboxyl-terminal hydrolase CYLD
                     isoform X1"
                     /calculated_mol_wt=164708
     Region          1128..1478
                     /region_name="Peptidase_C19"
                     /note="Peptidase C19 contains ubiquitinyl hydrolases. They
                     are intracellular peptidases that remove ubiquitin
                     molecules from polyubiquinated peptides by cleavage of
                     isopeptide bonds. They hydrolyse bonds involving the
                     carboxyl group of the C-terminal Gly...; cl02553"
                     /db_xref="CDD:470612"
     Site            order(1131,1136,1394,1416)
                     /site_type="active"
                     /db_xref="CDD:239135"
     CDS             1..1486
                     /gene="CYLD"
                     /coded_by="XM_077647435.1:666..5126"
                     /db_xref="GeneID:144114007"
ORIGIN      
        1 mfwlrqhvlp liegfseere rspgaesfrs alsdgaesfy salsdhrssv yydaessdld
       61 ssdsdsryad vdeelfseqm pevgtdykea vakertvdda yesfsygklf edvygsvvhr
      121 ppllrrqqth qgsqnemqpp prkwrpppri pvkvdlfqec avpavnhepp ahcrsqpvef
      181 tghveheddw qemesaeleg emqegevsap llsyleqaae efaefildea reeacrfvna
      241 vsvigdrgki arpslkslps dnggqtelle cavtvangta qhtatsqdqd ildafnrqfe
      301 pcfdseeeld pqafenased didntgqsdi eekgllnrna llgtfkrfpk sllkslphvh
      361 sfsdgsrngg sprrcpsplf pvtssasdlt ekywrrhssd iadtgvqpkg lnstnknfls
      421 sdpdistypc tslsnghgdg amvdvlnmtm gasfplepqk kinsvkaidi aqclpvthpe
      481 klleesqsef ssalpsavll henkgngfap mqekcgalls eppkppprsp rhsnvmqpfq
      541 cqqisqeyts kvdcfseigm aqvenqaipe ggpcleshlk vparrkktmp gfqsfrgisv
      601 edspssekvs asaadplllk drstvedstk eeqlvqdkvp lfeqgflhrv tcdeseedss
      661 fsssedpqtd vempsngnlg llsyedpepd gepptsgilr lpfslsqltg hlfeddslld
      721 vvsfhvvdnc dflkdtcdds ppyvvklgps ddfsaflvdp dtsehseldg sgtdsvfeda
      781 asqsfnesnl dsfqsgcvtp dennekgded fenqqsgkqr dqsksfpnip arprrkcaqt
      841 sliekqhsti stgqcvdlkm hdkisaelgs petnasrnms civtsipdep qsthmpyvqc
      901 qggnlvcnni raetlqehsm leadvhrked vlemsgskea agalsgitpr ptsssyqevp
      961 gaglecdkpi lektsggslv gaalvgadss egsrsqnsvm tnsgemnsts gkadydvlqe
     1021 akrvietacd rdlvqatlkh fkdfakdeqg pggsprpppk latsdavnhv nplyvkyqas
     1081 kakaeatpcs tssteedgsn sspetndlap vmgqtlsqvs emcrtnagiq ghhnscylda
     1141 tlfamfscty vfddiltrkr dpsedikeyd qiqkvlrddi vctlrrkkyv phenvmklre
     1201 lldslgnvsg ltteekdpee flnslihalr vppflklssq qethlyqlfv tkhellerpt
     1261 vqqlfhqsih esriklkeip kaliiqmprc gkqfklydli vpslelditd alensprfcy
     1321 vcgkqathec wdcfrpdvgl evisycrscs dtvhkhhsre thktatfend fgegatprki
     1381 smrlfaivci etshyvcfvk cpvkdstkpe wcffdsmadr eggedghnip kvekyadaev
     1441 wlgpkglekl rgisensiqm lpgktrrvfr daymcmyenp lvmkyk
//
LOCUS       XP_077503562            1467 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin carboxyl-terminal hydrolase CYLD isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503562
VERSION     XP_077503562.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647436.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1467
                     /product="ubiquitin carboxyl-terminal hydrolase CYLD
                     isoform X2"
                     /calculated_mol_wt=162596
     Region          1109..1459
                     /region_name="Peptidase_C19"
                     /note="Peptidase C19 contains ubiquitinyl hydrolases. They
                     are intracellular peptidases that remove ubiquitin
                     molecules from polyubiquinated peptides by cleavage of
                     isopeptide bonds. They hydrolyse bonds involving the
                     carboxyl group of the C-terminal Gly...; cl02553"
                     /db_xref="CDD:470612"
     Site            order(1112,1117,1375,1397)
                     /site_type="active"
                     /db_xref="CDD:239135"
     CDS             1..1467
                     /gene="CYLD"
                     /coded_by="XM_077647436.1:666..5069"
                     /db_xref="GeneID:144114007"
ORIGIN      
        1 mfwlrqhvlp liegfseere rspgaesfrs alsdgaesfy salsdhrssv yydaessdld
       61 ssdsdsryad vdeelfseqm pevgtdykea vakertvdda yesfsygklf edvygsvvhr
      121 ppllrrqqth qgsqnemqpp prkwrpppri pvkvdlfqec avpavnhepp ahcrsqpvef
      181 tghveheddw qemesaeleg emqegevsap llsyleqaae efaefildea reeacrfvna
      241 vsvigdrgki arpslkslps dnggqtelle cavtvangta qhtatsqdqd ildafnrqfe
      301 pcfdseeeld pqafenased didntgqsdi eekgllnrna llgtfkrfpk sllkslphvh
      361 sfsdgsrngg sprrcpsplf pvtssasdlt ekywrrhssd iadtgvqpkg lnstnknfls
      421 sdpdistypc tslsnghgdg amvdvlnmtm gasfplepqk kinsvkaidi aqclpvthpe
      481 klleesqsef ssalpsavll henkgngfap mqekcgalls eppkppprsp rhsnvmqpfq
      541 cqqisqeyts kvdcfseigm aqvenqaipe ggpcleshlk vparrkktmp gfqsfrgisv
      601 edspssekvs asaadplllk drstvedstk eeqlvqdkvp lfeqgflhrv tcdeseedss
      661 fsssedpqtd vempsngnlg llsyedpepd gepptsgilr lpfslsqltg hlfeddslld
      721 vvsfhvvdnc dflkdtcdds ppyvvklgps ddfsaflvdp dtsehseldg sgtdsvfeda
      781 asqsfnesnl dsfqsgcvtp dennekgded fenqqsgkqr dqsksfpnip arprrkcaqt
      841 sliekqhsti stgqcvdlkm hdkisaelgs petnasrnms civtsipdep qsthmpyvqc
      901 qggnlvcnni raetlqehsm leadvhrked vlemsgskea agalsgitpr ptsssyqevp
      961 gaglecdkpi lektsggslv gaalvgadss egsrsqnsvm tnsgemnsts gkadydvlqe
     1021 akrvietacd rdlvqatlkh fkdfakdeqg pggsprpppt skakaeatpc stssteedgs
     1081 nsspetndla pvmgqtlsqv semcrtnagi qghhnscyld atlfamfsct yvfddiltrk
     1141 rdpsedikey dqiqkvlrdd ivctlrrkky vphenvmklr elldslgnvs gltteekdpe
     1201 eflnslihal rvppflklss qqethlyqlf vtkhellerp tvqqlfhqsi hesriklkei
     1261 pkaliiqmpr cgkqfklydl ivpsleldit dalensprfc yvcgkqathe cwdcfrpdvg
     1321 levisycrsc sdtvhkhhsr ethktatfen dfgegatprk ismrlfaivc ietshyvcfv
     1381 kcpvkdstkp ewcffdsmad reggedghni pkvekyadae vwlgpkglek lrgisensiq
     1441 mlpgktrrvf rdaymcmyen plvmkyk
//
LOCUS       XP_077503563            1296 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin carboxyl-terminal hydrolase CYLD isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503563
VERSION     XP_077503563.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647437.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1296
                     /product="ubiquitin carboxyl-terminal hydrolase CYLD
                     isoform X3"
                     /calculated_mol_wt=144539
     Region          938..1288
                     /region_name="Peptidase_C19"
                     /note="Peptidase C19 contains ubiquitinyl hydrolases. They
                     are intracellular peptidases that remove ubiquitin
                     molecules from polyubiquinated peptides by cleavage of
                     isopeptide bonds. They hydrolyse bonds involving the
                     carboxyl group of the C-terminal Gly...; cl02553"
                     /db_xref="CDD:470612"
     Site            order(941,946,1204,1226)
                     /site_type="active"
                     /db_xref="CDD:239135"
     CDS             1..1296
                     /gene="CYLD"
                     /coded_by="XM_077647437.1:578..4468"
                     /db_xref="GeneID:144114007"
ORIGIN      
        1 mfwlrqhvlp liegfseere rspgaesfrs alsdgaesfy salsdhrssv yydaessdld
       61 ssdsdsryad vdeelfseqm pevgtdykea vakertvdda yesfsygklf edvygsvvhr
      121 ppllrrqqth qgsqnemqpp prkwrpppri pvkvdlfqec avpavnhepp ahcrsqpvef
      181 tghveheddw qemesaeleg emqegevsap llsyleqaae efaefildea reeacrfvna
      241 vsvigdrgki arpslkslps dnggqtelle cavtvangta qhtatsqdqd ildafnrqfe
      301 pcfdseeeld pqafenased didntgqsdi eekgllnrna llgtfkrfpk sllkslphvh
      361 sfsdgsrngg sprrcpsplf pvtssasdlt ekywrrhssd iadtgvqpkg lnstnknfls
      421 sdpdistypc tslsnghgdg amvdvlnmtm gasfplepqk kinsvkaidi aqclpvthpe
      481 klleesqsef ssalpsavll henkgngfap mqekcgalls eppkppprsp rhsnvmqpfq
      541 cqqisqeyts kvdcfseigm aqvenqaipe ggpcleshlk vparrkktmp gfqsfrgisv
      601 edspssekvs asaadplllk drstvedstk eeqlvqdkvp lfeqgflhrv tcdeseedss
      661 fsssedpqtd vempsngnlg llsyedpepd gepptsgilr lpfslsqltg hlfeddslld
      721 vvsfhvvdnc dflkdtcdds ppyvvklgps ddfsaflvdp dtsehseldg sgtdsvfeda
      781 asqsfnesnl dsfqsgcvtp dennekgded fenqqsgkqr dqsksfpnip arprrkcaqt
      841 sliekqhsti stdfakdeqg pggsprpppk latsdavnhv nplyvkyqas kakaeatpcs
      901 tssteedgsn sspetndlap vmgqtlsqvs emcrtnagiq ghhnscylda tlfamfscty
      961 vfddiltrkr dpsedikeyd qiqkvlrddi vctlrrkkyv phenvmklre lldslgnvsg
     1021 ltteekdpee flnslihalr vppflklssq qethlyqlfv tkhellerpt vqqlfhqsih
     1081 esriklkeip kaliiqmprc gkqfklydli vpslelditd alensprfcy vcgkqathec
     1141 wdcfrpdvgl evisycrscs dtvhkhhsre thktatfend fgegatprki smrlfaivci
     1201 etshyvcfvk cpvkdstkpe wcffdsmadr eggedghnip kvekyadaev wlgpkglekl
     1261 rgisensiqm lpgktrrvfr daymcmyenp lvmkyk
//
LOCUS       XP_077503564            1277 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin carboxyl-terminal hydrolase CYLD isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503564
VERSION     XP_077503564.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647438.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1277
                     /product="ubiquitin carboxyl-terminal hydrolase CYLD
                     isoform X4"
                     /calculated_mol_wt=142427
     Region          919..1269
                     /region_name="Peptidase_C19"
                     /note="Peptidase C19 contains ubiquitinyl hydrolases. They
                     are intracellular peptidases that remove ubiquitin
                     molecules from polyubiquinated peptides by cleavage of
                     isopeptide bonds. They hydrolyse bonds involving the
                     carboxyl group of the C-terminal Gly...; cl02553"
                     /db_xref="CDD:470612"
     Site            order(922,927,1185,1207)
                     /site_type="active"
                     /db_xref="CDD:239135"
     CDS             1..1277
                     /gene="CYLD"
                     /coded_by="XM_077647438.1:142..3975"
                     /db_xref="GeneID:144114007"
ORIGIN      
        1 mfwlrqhvlp liegfseere rspgaesfrs alsdgaesfy salsdhrssv yydaessdld
       61 ssdsdsryad vdeelfseqm pevgtdykea vakertvdda yesfsygklf edvygsvvhr
      121 ppllrrqqth qgsqnemqpp prkwrpppri pvkvdlfqec avpavnhepp ahcrsqpvef
      181 tghveheddw qemesaeleg emqegevsap llsyleqaae efaefildea reeacrfvna
      241 vsvigdrgki arpslkslps dnggqtelle cavtvangta qhtatsqdqd ildafnrqfe
      301 pcfdseeeld pqafenased didntgqsdi eekgllnrna llgtfkrfpk sllkslphvh
      361 sfsdgsrngg sprrcpsplf pvtssasdlt ekywrrhssd iadtgvqpkg lnstnknfls
      421 sdpdistypc tslsnghgdg amvdvlnmtm gasfplepqk kinsvkaidi aqclpvthpe
      481 klleesqsef ssalpsavll henkgngfap mqekcgalls eppkppprsp rhsnvmqpfq
      541 cqqisqeyts kvdcfseigm aqvenqaipe ggpcleshlk vparrkktmp gfqsfrgisv
      601 edspssekvs asaadplllk drstvedstk eeqlvqdkvp lfeqgflhrv tcdeseedss
      661 fsssedpqtd vempsngnlg llsyedpepd gepptsgilr lpfslsqltg hlfeddslld
      721 vvsfhvvdnc dflkdtcdds ppyvvklgps ddfsaflvdp dtsehseldg sgtdsvfeda
      781 asqsfnesnl dsfqsgcvtp dennekgded fenqqsgkqr dqsksfpnip arprrkcaqt
      841 sliekqhsti stdfakdeqg pggsprpppt skakaeatpc stssteedgs nsspetndla
      901 pvmgqtlsqv semcrtnagi qghhnscyld atlfamfsct yvfddiltrk rdpsedikey
      961 dqiqkvlrdd ivctlrrkky vphenvmklr elldslgnvs gltteekdpe eflnslihal
     1021 rvppflklss qqethlyqlf vtkhellerp tvqqlfhqsi hesriklkei pkaliiqmpr
     1081 cgkqfklydl ivpsleldit dalensprfc yvcgkqathe cwdcfrpdvg levisycrsc
     1141 sdtvhkhhsr ethktatfen dfgegatprk ismrlfaivc ietshyvcfv kcpvkdstkp
     1201 ewcffdsmad reggedghni pkvekyadae vwlgpkglek lrgisensiq mlpgktrrvf
     1261 rdaymcmyen plvmkyk
//
LOCUS       XP_077503565             770 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin carboxyl-terminal hydrolase CYLD isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077503565
VERSION     XP_077503565.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647439.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..770
                     /product="ubiquitin carboxyl-terminal hydrolase CYLD
                     isoform X5"
                     /calculated_mol_wt=87585
     Region          207..272
                     /region_name="CAP_GLY"
                     /note="Cytoskeleton-associated proteins (CAPs) are
                     involved in the organisation of microtubules and
                     transportation of vesicles and organelles along the
                     cytoskeletal network; smart01052"
                     /db_xref="CDD:214997"
     Region          412..762
                     /region_name="Peptidase_C19"
                     /note="Peptidase C19 contains ubiquitinyl hydrolases. They
                     are intracellular peptidases that remove ubiquitin
                     molecules from polyubiquinated peptides by cleavage of
                     isopeptide bonds. They hydrolyse bonds involving the
                     carboxyl group of the C-terminal Gly...; cl02553"
                     /db_xref="CDD:470612"
     Site            order(415,420,678,700)
                     /site_type="active"
                     /db_xref="CDD:239135"
     CDS             1..770
                     /gene="CYLD"
                     /coded_by="XM_077647439.1:380..2692"
                     /db_xref="GeneID:144114007"
ORIGIN      
        1 mnrttqshkp kvlldrdiqn prfdagtlfe vladdpyesk vkvqsldpgd sgvilcnrei
       61 lrevtsleal vllpvkpvrn rlkiyrdqew lkeipiisen krvvvkdkfe givkfkgvvr
      121 eygpgirfiv eiqdgtndpi krkqcfsiqd lrlldgreiy hghnsvvprl pskkkslssg
      181 itrcdpapwh qerlrdfcds essplrindr vvwvsdhgpe ygtvrwlghl hdtsedlmvg
      241 vefdnqvgtg tgkyrdrqlf eaqlghasli pllgllradd ylgeqapavr ascweqaseq
      301 vdygnscfyt shldrrpkvt ssshfqdfak deqgpggspr pppklatsda vnhvnplyvk
      361 yqaskakaea tpcstsstee dgsnsspetn dlapvmgqtl sqvsemcrtn agiqghhnsc
      421 yldatlfamf sctyvfddil trkrdpsedi keydqiqkvl rddivctlrr kkyvphenvm
      481 klrelldslg nvsgltteek dpeeflnsli halrvppflk lssqqethly qlfvtkhell
      541 erptvqqlfh qsihesrikl keipkaliiq mprcgkqfkl ydlivpslel ditdalensp
      601 rfcyvcgkqa thecwdcfrp dvglevisyc rscsdtvhkh hsrethktat fendfgegat
      661 prkismrlfa ivcietshyv cfvkcpvkds tkpewcffds madreggedg hnipkvekya
      721 daevwlgpkg leklrgisen siqmlpgktr rvfrdaymcm yenplvmkyk
//
LOCUS       XP_077503566             757 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin carboxyl-terminal hydrolase CYLD isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077503566
VERSION     XP_077503566.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647440.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..757
                     /product="ubiquitin carboxyl-terminal hydrolase CYLD
                     isoform X6"
                     /calculated_mol_wt=86138
     Region          207..272
                     /region_name="CAP_GLY"
                     /note="Cytoskeleton-associated proteins (CAPs) are
                     involved in the organisation of microtubules and
                     transportation of vesicles and organelles along the
                     cytoskeletal network; smart01052"
                     /db_xref="CDD:214997"
     Region          399..749
                     /region_name="Peptidase_C19"
                     /note="Peptidase C19 contains ubiquitinyl hydrolases. They
                     are intracellular peptidases that remove ubiquitin
                     molecules from polyubiquinated peptides by cleavage of
                     isopeptide bonds. They hydrolyse bonds involving the
                     carboxyl group of the C-terminal Gly...; cl02553"
                     /db_xref="CDD:470612"
     Site            order(402,407,665,687)
                     /site_type="active"
                     /db_xref="CDD:239072"
     CDS             1..757
                     /gene="CYLD"
                     /coded_by="XM_077647440.1:380..2653"
                     /db_xref="GeneID:144114007"
ORIGIN      
        1 mnrttqshkp kvlldrdiqn prfdagtlfe vladdpyesk vkvqsldpgd sgvilcnrei
       61 lrevtsleal vllpvkpvrn rlkiyrdqew lkeipiisen krvvvkdkfe givkfkgvvr
      121 eygpgirfiv eiqdgtndpi krkqcfsiqd lrlldgreiy hghnsvvprl pskkkslssg
      181 itrcdpapwh qerlrdfcds essplrindr vvwvsdhgpe ygtvrwlghl hdtsedlmvg
      241 vefdnqvgtg tgkyrdrqlf eaqlghasli pllgllradd ylgeqapvdy gnscfytshl
      301 drrpkvtsss hfqdfakdeq gpggsprppp klatsdavnh vnplyvkyqa skakaeatpc
      361 stssteedgs nsspetndla pvmgqtlsqv semcrtnagi qghhnscyld atlfamfsct
      421 yvfddiltrk rdpsedikey dqiqkvlrdd ivctlrrkky vphenvmklr elldslgnvs
      481 gltteekdpe eflnslihal rvppflklss qqethlyqlf vtkhellerp tvqqlfhqsi
      541 hesriklkei pkaliiqmpr cgkqfklydl ivpsleldit dalensprfc yvcgkqathe
      601 cwdcfrpdvg levisycrsc sdtvhkhhsr ethktatfen dfgegatprk ismrlfaivc
      661 ietshyvcfv kcpvkdstkp ewcffdsmad reggedghni pkvekyadae vwlgpkglek
      721 lrgisensiq mlpgktrrvf rdaymcmyen plvmkyk
//
LOCUS       XP_077503567             751 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin carboxyl-terminal hydrolase CYLD isoform X7 [Amblyomma
            americanum].
ACCESSION   XP_077503567
VERSION     XP_077503567.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647441.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..751
                     /product="ubiquitin carboxyl-terminal hydrolase CYLD
                     isoform X7"
                     /calculated_mol_wt=85473
     Region          207..272
                     /region_name="CAP_GLY"
                     /note="Cytoskeleton-associated proteins (CAPs) are
                     involved in the organisation of microtubules and
                     transportation of vesicles and organelles along the
                     cytoskeletal network; smart01052"
                     /db_xref="CDD:214997"
     Region          393..743
                     /region_name="Peptidase_C19"
                     /note="Peptidase C19 contains ubiquitinyl hydrolases. They
                     are intracellular peptidases that remove ubiquitin
                     molecules from polyubiquinated peptides by cleavage of
                     isopeptide bonds. They hydrolyse bonds involving the
                     carboxyl group of the C-terminal Gly...; cl02553"
                     /db_xref="CDD:470612"
     Site            order(396,401,659,681)
                     /site_type="active"
                     /db_xref="CDD:239135"
     CDS             1..751
                     /gene="CYLD"
                     /coded_by="XM_077647441.1:380..2635"
                     /db_xref="GeneID:144114007"
ORIGIN      
        1 mnrttqshkp kvlldrdiqn prfdagtlfe vladdpyesk vkvqsldpgd sgvilcnrei
       61 lrevtsleal vllpvkpvrn rlkiyrdqew lkeipiisen krvvvkdkfe givkfkgvvr
      121 eygpgirfiv eiqdgtndpi krkqcfsiqd lrlldgreiy hghnsvvprl pskkkslssg
      181 itrcdpapwh qerlrdfcds essplrindr vvwvsdhgpe ygtvrwlghl hdtsedlmvg
      241 vefdnqvgtg tgkyrdrqlf eaqlghasli pllgllradd ylgeqapavr ascweqaseq
      301 vdygnscfyt shldrrpkvt ssshfqdfak deqgpggspr ppptskakae atpcstsste
      361 edgsnsspet ndlapvmgqt lsqvsemcrt nagiqghhns cyldatlfam fsctyvfddi
      421 ltrkrdpsed ikeydqiqkv lrddivctlr rkkyvphenv mklrelldsl gnvsglttee
      481 kdpeeflnsl ihalrvppfl klssqqethl yqlfvtkhel lerptvqqlf hqsihesrik
      541 lkeipkalii qmprcgkqfk lydlivpsle lditdalens prfcyvcgkq athecwdcfr
      601 pdvglevisy crscsdtvhk hhsrethkta tfendfgega tprkismrlf aivcietshy
      661 vcfvkcpvkd stkpewcffd smadregged ghnipkveky adaevwlgpk gleklrgise
      721 nsiqmlpgkt rrvfrdaymc myenplvmky k
//
LOCUS       XP_077503568             738 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin carboxyl-terminal hydrolase CYLD isoform X8 [Amblyomma
            americanum].
ACCESSION   XP_077503568
VERSION     XP_077503568.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647442.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..738
                     /product="ubiquitin carboxyl-terminal hydrolase CYLD
                     isoform X8"
                     /calculated_mol_wt=84026
     Region          207..272
                     /region_name="CAP_GLY"
                     /note="Cytoskeleton-associated proteins (CAPs) are
                     involved in the organisation of microtubules and
                     transportation of vesicles and organelles along the
                     cytoskeletal network; smart01052"
                     /db_xref="CDD:214997"
     Region          380..730
                     /region_name="Peptidase_C19"
                     /note="Peptidase C19 contains ubiquitinyl hydrolases. They
                     are intracellular peptidases that remove ubiquitin
                     molecules from polyubiquinated peptides by cleavage of
                     isopeptide bonds. They hydrolyse bonds involving the
                     carboxyl group of the C-terminal Gly...; cl02553"
                     /db_xref="CDD:470612"
     Site            order(383,388,646,668)
                     /site_type="active"
                     /db_xref="CDD:239135"
     CDS             1..738
                     /gene="CYLD"
                     /coded_by="XM_077647442.1:380..2596"
                     /db_xref="GeneID:144114007"
ORIGIN      
        1 mnrttqshkp kvlldrdiqn prfdagtlfe vladdpyesk vkvqsldpgd sgvilcnrei
       61 lrevtsleal vllpvkpvrn rlkiyrdqew lkeipiisen krvvvkdkfe givkfkgvvr
      121 eygpgirfiv eiqdgtndpi krkqcfsiqd lrlldgreiy hghnsvvprl pskkkslssg
      181 itrcdpapwh qerlrdfcds essplrindr vvwvsdhgpe ygtvrwlghl hdtsedlmvg
      241 vefdnqvgtg tgkyrdrqlf eaqlghasli pllgllradd ylgeqapvdy gnscfytshl
      301 drrpkvtsss hfqdfakdeq gpggsprppp tskakaeatp cstssteedg snsspetndl
      361 apvmgqtlsq vsemcrtnag iqghhnscyl datlfamfsc tyvfddiltr krdpsedike
      421 ydqiqkvlrd divctlrrkk yvphenvmkl relldslgnv sgltteekdp eeflnsliha
      481 lrvppflkls sqqethlyql fvtkheller ptvqqlfhqs ihesriklke ipkaliiqmp
      541 rcgkqfklyd livpsleldi tdalensprf cyvcgkqath ecwdcfrpdv glevisycrs
      601 csdtvhkhhs rethktatfe ndfgegatpr kismrlfaiv cietshyvcf vkcpvkdstk
      661 pewcffdsma dreggedghn ipkvekyada evwlgpkgle klrgisensi qmlpgktrrv
      721 frdaymcmye nplvmkyk
//
LOCUS       XP_077503569             633 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin carboxyl-terminal hydrolase CYLD isoform X9 [Amblyomma
            americanum].
ACCESSION   XP_077503569
VERSION     XP_077503569.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647443.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..633
                     /product="ubiquitin carboxyl-terminal hydrolase CYLD
                     isoform X9"
                     /calculated_mol_wt=71793
     Region          70..135
                     /region_name="CAP_GLY"
                     /note="Cytoskeleton-associated proteins (CAPs) are
                     involved in the organisation of microtubules and
                     transportation of vesicles and organelles along the
                     cytoskeletal network; smart01052"
                     /db_xref="CDD:214997"
     Region          275..625
                     /region_name="Peptidase_C19"
                     /note="Peptidase C19 contains ubiquitinyl hydrolases. They
                     are intracellular peptidases that remove ubiquitin
                     molecules from polyubiquinated peptides by cleavage of
                     isopeptide bonds. They hydrolyse bonds involving the
                     carboxyl group of the C-terminal Gly...; cl02553"
                     /db_xref="CDD:470612"
     Site            order(278,283,541,563)
                     /site_type="active"
                     /db_xref="CDD:239135"
     CDS             1..633
                     /gene="CYLD"
                     /coded_by="XM_077647443.1:117..2018"
                     /db_xref="GeneID:144114007"
ORIGIN      
        1 mncmcskkrr prgdgdmylp rdphghnsvv prlpskkksl ssgitrcdpa pwhqerlrdf
       61 cdsessplri ndrvvwvsdh gpeygtvrwl ghlhdtsedl mvgvefdnqv gtgtgkyrdr
      121 qlfeaqlgha slipllgllr addylgeqap avrascweqa seqvdygnsc fytshldrrp
      181 kvtssshfqd fakdeqgpgg sprpppklat sdavnhvnpl yvkyqaskak aeatpcstss
      241 teedgsnssp etndlapvmg qtlsqvsemc rtnagiqghh nscyldatlf amfsctyvfd
      301 diltrkrdps edikeydqiq kvlrddivct lrrkkyvphe nvmklrelld slgnvsgltt
      361 eekdpeefln slihalrvpp flklssqqet hlyqlfvtkh ellerptvqq lfhqsihesr
      421 iklkeipkal iiqmprcgkq fklydlivps lelditdale nsprfcyvcg kqathecwdc
      481 frpdvglevi sycrscsdtv hkhhsrethk tatfendfge gatprkismr lfaivciets
      541 hyvcfvkcpv kdstkpewcf fdsmadregg edghnipkve kyadaevwlg pkgleklrgi
      601 sensiqmlpg ktrrvfrday mcmyenplvm kyk
//
LOCUS       XP_077503570             620 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin carboxyl-terminal hydrolase CYLD isoform X10 [Amblyomma
            americanum].
ACCESSION   XP_077503570
VERSION     XP_077503570.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647444.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..620
                     /product="ubiquitin carboxyl-terminal hydrolase CYLD
                     isoform X10"
                     /calculated_mol_wt=70346
     Region          70..135
                     /region_name="CAP_GLY"
                     /note="Cytoskeleton-associated proteins (CAPs) are
                     involved in the organisation of microtubules and
                     transportation of vesicles and organelles along the
                     cytoskeletal network; smart01052"
                     /db_xref="CDD:214997"
     Region          262..612
                     /region_name="Peptidase_C19"
                     /note="Peptidase C19 contains ubiquitinyl hydrolases. They
                     are intracellular peptidases that remove ubiquitin
                     molecules from polyubiquinated peptides by cleavage of
                     isopeptide bonds. They hydrolyse bonds involving the
                     carboxyl group of the C-terminal Gly...; cl02553"
                     /db_xref="CDD:470612"
     Site            order(265,270,528,550)
                     /site_type="active"
                     /db_xref="CDD:239072"
     CDS             1..620
                     /gene="CYLD"
                     /coded_by="XM_077647444.1:115..1977"
                     /db_xref="GeneID:144114007"
ORIGIN      
        1 mncmcskkrr prgdgdmylp rdphghnsvv prlpskkksl ssgitrcdpa pwhqerlrdf
       61 cdsessplri ndrvvwvsdh gpeygtvrwl ghlhdtsedl mvgvefdnqv gtgtgkyrdr
      121 qlfeaqlgha slipllgllr addylgeqap vdygnscfyt shldrrpkvt ssshfqdfak
      181 deqgpggspr pppklatsda vnhvnplyvk yqaskakaea tpcstsstee dgsnsspetn
      241 dlapvmgqtl sqvsemcrtn agiqghhnsc yldatlfamf sctyvfddil trkrdpsedi
      301 keydqiqkvl rddivctlrr kkyvphenvm klrelldslg nvsgltteek dpeeflnsli
      361 halrvppflk lssqqethly qlfvtkhell erptvqqlfh qsihesrikl keipkaliiq
      421 mprcgkqfkl ydlivpslel ditdalensp rfcyvcgkqa thecwdcfrp dvglevisyc
      481 rscsdtvhkh hsrethktat fendfgegat prkismrlfa ivcietshyv cfvkcpvkds
      541 tkpewcffds madreggedg hnipkvekya daevwlgpkg leklrgisen siqmlpgktr
      601 rvfrdaymcm yenplvmkyk
//
LOCUS       XP_077503571             601 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin carboxyl-terminal hydrolase CYLD isoform X11 [Amblyomma
            americanum].
ACCESSION   XP_077503571
VERSION     XP_077503571.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647445.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..601
                     /product="ubiquitin carboxyl-terminal hydrolase CYLD
                     isoform X11"
                     /calculated_mol_wt=68234
     Region          70..135
                     /region_name="CAP_GLY"
                     /note="Cytoskeleton-associated proteins (CAPs) are
                     involved in the organisation of microtubules and
                     transportation of vesicles and organelles along the
                     cytoskeletal network; smart01052"
                     /db_xref="CDD:214997"
     Region          243..593
                     /region_name="Peptidase_C19"
                     /note="Peptidase C19 contains ubiquitinyl hydrolases. They
                     are intracellular peptidases that remove ubiquitin
                     molecules from polyubiquinated peptides by cleavage of
                     isopeptide bonds. They hydrolyse bonds involving the
                     carboxyl group of the C-terminal Gly...; cl02553"
                     /db_xref="CDD:470612"
     Site            order(246,251,509,531)
                     /site_type="active"
                     /db_xref="CDD:239135"
     CDS             1..601
                     /gene="CYLD"
                     /coded_by="XM_077647445.1:76..1881"
                     /db_xref="GeneID:144114007"
ORIGIN      
        1 mncmcskkrr prgdgdmylp rdphghnsvv prlpskkksl ssgitrcdpa pwhqerlrdf
       61 cdsessplri ndrvvwvsdh gpeygtvrwl ghlhdtsedl mvgvefdnqv gtgtgkyrdr
      121 qlfeaqlgha slipllgllr addylgeqap vdygnscfyt shldrrpkvt ssshfqdfak
      181 deqgpggspr ppptskakae atpcstsste edgsnsspet ndlapvmgqt lsqvsemcrt
      241 nagiqghhns cyldatlfam fsctyvfddi ltrkrdpsed ikeydqiqkv lrddivctlr
      301 rkkyvphenv mklrelldsl gnvsglttee kdpeeflnsl ihalrvppfl klssqqethl
      361 yqlfvtkhel lerptvqqlf hqsihesrik lkeipkalii qmprcgkqfk lydlivpsle
      421 lditdalens prfcyvcgkq athecwdcfr pdvglevisy crscsdtvhk hhsrethkta
      481 tfendfgega tprkismrlf aivcietshy vcfvkcpvkd stkpewcffd smadregged
      541 ghnipkveky adaevwlgpk gleklrgise nsiqmlpgkt rrvfrdaymc myenplvmky
      601 k
//
LOCUS       XP_077503572             366 aa            linear   INV 25-NOV-2025
DEFINITION  cyclin-dependent kinase 10 [Amblyomma americanum].
ACCESSION   XP_077503572
VERSION     XP_077503572.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647446.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..366
                     /product="cyclin-dependent kinase 10"
                     /calculated_mol_wt=41217
     Region          34..342
                     /region_name="STKc_CDK10"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Cyclin-Dependent protein Kinase 10; cd07845"
                     /db_xref="CDD:173742"
     Site            order(48..51,56,69,71,88,102,120..123,126,128..129,166,
                     168,170..171,173,184,187,199,201..204,206,244..245)
                     /site_type="active"
                     /db_xref="CDD:173742"
     Site            order(48..51,56,69,71,102,120..123,126,129,170..171,173,
                     184)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:173742"
     Site            order(80..82,84,87..88,90..91,94..95,107,109,116,155,
                     158..161,189,191..194,198..199,312,316..318)
                     /site_type="other"
                     /note="CDK/cyclin interface [polypeptide binding]"
                     /db_xref="CDD:173742"
     Site            order(88,128,166,168,187,199,201..204,206,244..245)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:173742"
     Site            order(183..194,196..206)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:173742"
     CDS             1..366
                     /gene="Cdc2rk"
                     /coded_by="XM_077647446.1:71..1171"
                     /db_xref="GeneID:144114009"
ORIGIN      
        1 meaglnqaap ekrvvlasls tgkffeipdk diagrcrlvt dfeklnrige gtygivyraq
       61 dlssgevvam kkvrmeqekd gipvsglrei nlllniqhqn ivnlkevavg kslesiflvm
      121 eyceqdlasl ldnmqspfse sqvkcimmql fkglqylhkn fivhrdlkvs nllltdkgcl
      181 kiadfglark ygvpvkpmtp rvvtlwyrap elllqaktqt taidiwaagc vlgelllhkp
      241 llpgrseihq leliidllgt pndmiwpgys klpalenftl kqqpynnlkh ffswlspagi
      301 rllnflfmyd pkkrataees lqssyfsepp lpceaelmps fpqhrnlkrt gvgdlseemk
      361 ktakli
//
LOCUS       XP_077503573             165 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114012 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503573
VERSION     XP_077503573.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647447.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..165
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..165
                     /product="uncharacterized protein LOC144114012 isoform X1"
                     /calculated_mol_wt=17767
     CDS             1..165
                     /gene="LOC144114012"
                     /coded_by="XM_077647447.1:104..601"
                     /db_xref="GeneID:144114012"
ORIGIN      
        1 mglprldvwf agtnddlppf sfalslsfln spsgvltvae ivtgiltfal ayssrvyvyq
       61 lsklvvrvep epsiiyvifv sflywfvsll vlaaalvsnt ginvtstffy vlleafgfif
      121 ylsggislla leasqsvata agvfsiisav lhglhallvy lkkkk
//
LOCUS       XP_077503574             165 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114012 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503574
VERSION     XP_077503574.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647448.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..165
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..165
                     /product="uncharacterized protein LOC144114012 isoform X1"
                     /calculated_mol_wt=17767
     CDS             1..165
                     /gene="LOC144114012"
                     /coded_by="XM_077647448.1:104..601"
                     /db_xref="GeneID:144114012"
ORIGIN      
        1 mglprldvwf agtnddlppf sfalslsfln spsgvltvae ivtgiltfal ayssrvyvyq
       61 lsklvvrvep epsiiyvifv sflywfvsll vlaaalvsnt ginvtstffy vlleafgfif
      121 ylsggislla leasqsvata agvfsiisav lhglhallvy lkkkk
//
LOCUS       XP_077503575             132 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114012 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503575
VERSION     XP_077503575.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647449.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..132
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..132
                     /product="uncharacterized protein LOC144114012 isoform X2"
                     /calculated_mol_wt=14405
     CDS             1..132
                     /gene="LOC144114012"
                     /coded_by="XM_077647449.1:110..508"
                     /db_xref="GeneID:144114012"
ORIGIN      
        1 mglprldvwf agtnddlppf sfalslsfln spsgvltvae ivtgiltfal ayssrvyvyq
       61 lsklvvrvep epsiiyvifv sflywfvsll vlaaalvsnt ginvtstffv fsiisavlhg
      121 lhallvylkk kk
//
LOCUS       XP_077503576             544 aa            linear   INV 25-NOV-2025
DEFINITION  luciferin 4-monooxygenase-like [Amblyomma americanum].
ACCESSION   XP_077503576
VERSION     XP_077503576.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647450.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..544
                     /product="luciferin 4-monooxygenase-like"
                     /calculated_mol_wt=59154
     Region          46..525
                     /region_name="AFD_class_I"
                     /note="Adenylate forming domain, Class I superfamily;
                     cl17068"
                     /db_xref="CDD:473059"
     Site            order(189,192..197,199..200)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341228"
     Site            order(192,308..309,331..336,415,427,430,441,525)
                     /site_type="other"
                     /note="AMP binding site [chemical binding]"
                     /db_xref="CDD:341228"
     Site            order(192,234..235,281,283..284,287,308..309,331..336,415,
                     427,430,438..441,506)
                     /site_type="active"
                     /db_xref="CDD:341228"
     Site            order(234,283..284,287,308,438..440,499,506)
                     /site_type="other"
                     /note="CoA binding site [chemical binding]"
                     /db_xref="CDD:341228"
     CDS             1..544
                     /gene="LOC144114014"
                     /coded_by="XM_077647450.1:419..2053"
                     /db_xref="GeneID:144114014"
ORIGIN      
        1 mdrvvddapv iedrivkgys pgqpipdvdy aaylrtlwgq fkdrtalika dtgerytyhe
       61 ledacsrvag glaslgfqpg dmagihcdts leamvafygt afaggsmvfa kgsltarevn
      121 yqfkdtkpci vfcdeenaak aaeacktips vktlvifgqn egmmpfstlr qscppaalps
      181 vdpdsllail yssgttglpk gtllsnrnav aslfssrndr tkaveqddvl lgsapfthvs
      241 gllfnnaifa hgaclviias lepsdmlpai ekykgtimfq fptflkklvr spllekydis
      301 svkkiyaggs atpsviveet lrkfnlehfg qaysmsesfg sgmisrrdck dyesvgtatt
      361 lteikivdvn tgaklgprqt geirirsqsc vrgylnnpea taslydedgf lrsgdigyyn
      421 ergefyvvdr ikemfkcmdq qvapseiedm llqheavkda avagvphpef gdaprafvvl
      481 rkgyaasegt aaelralvsa kvarhkhlhg gvefvssipk sdtgkslrrd lrdaylqrqa
      541 vrgl
//
LOCUS       XP_077503578             434 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114016 [Amblyomma americanum].
ACCESSION   XP_077503578
VERSION     XP_077503578.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647452.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..434
                     /product="uncharacterized protein LOC144114016"
                     /calculated_mol_wt=48225
     CDS             1..434
                     /gene="LOC144114016"
                     /coded_by="XM_077647452.1:1..1305"
                     /db_xref="GeneID:144114016"
ORIGIN      
        1 mgfidkkswd snelsrrgmh nktfqrhlkn tlqpaatqaa aaamaqvaqa vakvyddlcf
       61 ghrgniamcy dgtwmtrghs shigvgavve lftgyvldyv llsnfclgce tgpklntggy
      121 elwrshhecq kntnckagqm eveagkilfe rslqrhnmry ttmlcdgdsr tfnvlqyaki
      181 ygyievvked cinhvhkrmg aalrnllqrh kgagkqslgg rgrltadlvd klaiyygqal
      241 rsnvgnvdam savmatyyhi tstddcpnht lcptgeqswc rhnaakakee pepkhkyslp
      301 sdvaeallpv ysrvseksll qrcirgktqn snesfhsiiw tlihkerhss lfaveaatae
      361 avlrfnagck htatailqec ngnmpatvsr rakgkdlrrn arsakkraas lsfakaekkk
      421 hrdsmhpdya pgaf
//
LOCUS       XP_077503579             967 aa            linear   INV 25-NOV-2025
DEFINITION  alanine--tRNA ligase, cytoplasmic [Amblyomma americanum].
ACCESSION   XP_077503579
VERSION     XP_077503579.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647453.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..967
                     /product="alanine--tRNA ligase, cytoplasmic"
                     /calculated_mol_wt=106655
     Region          3..963
                     /region_name="PLN02900"
                     /note="alanyl-tRNA synthetase"
                     /db_xref="CDD:215487"
     CDS             1..967
                     /gene="AlaRS"
                     /coded_by="XM_077647453.1:190..3093"
                     /db_xref="GeneID:144114017"
ORIGIN      
        1 mtsaaeirqk fmdffvqkyd hvyvksssvi phddptllft nagmnqfkpi flgtvdpnse
       61 agrykrvvdt qkciraggkh ndlddvgkdv yhhtffemlg nwsfgdffkk eicawawell
      121 tevfklqpdr lyvtyfggne aaglepdlec kdiwkklgvp edrilpgsmk dnfwemgetg
      181 pcgpcseihy driggrnaah lvnadvpdvl eiwnlvfiqy nretdgslrl lpkqhvdtgm
      241 gferlvsviq nkpsnydtdv fvpifeaiqk atgarpysgk vgkddvdgid mayrvladhs
      301 rtltialsdg grpdnigrgy vlrrilrrav ryatekmnaa pgtfaslvpv vvqilgdvfp
      361 elkkdpegvm diineeesqf lktlsrgrrl lektvgklgd qkflpgdvaw rlydtygfpi
      421 dltqlmveek glavdmesye kskaaaqlas qggdnkvddg lkldvhaise lqakgvpstd
      481 dlpkydywak sddrnavyef apcqgtvlvl rtckkfvdsv epgqqcgvil drtnfyaesg
      541 gqscdtgfmt cegnedvefa vtevhaqggy vvhigrlgsg tlkvgdklkl sideerrkll
      601 mnnhtgthvl nfalrkvlta eadqrgslva sdrlrfdfan kkpmspaevk aaeehaveli
      661 akngavyakd splslakaiq glravfnety pdpvrvvsig ipveemladp sspagsitsv
      721 efcggthlkr aghigdfvis teeaiakgir rivaltgpea tkalkkaehf qtevdrlrtd
      781 aekvtqaaqg kelvkqivel teeisqssis ywkkdelrte lknlkkkldd ldrnnkaqaq
      841 qlaietvkkl ledkkdcdyi vhtlevgdna kaldaalkqv rtltpnaaam ffsqdsekgk
      901 viclssvpkg ivargltana wvkevsdvig gkgggreesa qaignnlgsl ekalqvahdf
      961 aklklac
//
LOCUS       XP_077503580             694 aa            linear   INV 25-NOV-2025
DEFINITION  single-strand DNA endonuclease protein asteroid [Amblyomma
            americanum].
ACCESSION   XP_077503580
VERSION     XP_077503580.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647454.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..694
                     /product="single-strand DNA endonuclease protein asteroid"
                     /calculated_mol_wt=78296
     Region          27..168
                     /region_name="PIN_SF"
                     /note="PIN (PilT N terminus) domain: Superfamily; cl28905"
                     /db_xref="CDD:475124"
     Region          138..>226
                     /region_name="XPG_I_2"
                     /note="XPG domain containing; pfam12813"
                     /db_xref="CDD:432803"
     CDS             1..694
                     /gene="ast"
                     /coded_by="XM_077647454.1:226..2310"
                     /db_xref="GeneID:144114018"
ORIGIN      
        1 mgvhglssff tlnpdlsewk nlhstklvid gnnliymiyf tsnleciygg dydgyatair
       61 nyfaafkacn ievilvfdgg hdvsnrklkt tqrrlqerla taktiaktga pnlkilpila
      121 hevfkdvvre lgiqvlqcsf eadeeiaaia nyyecpvasq dsdfyifnlv ggfvrldsls
      181 atplavtmes gekvnvlscr wyhvdnllch fpgfdksllp lfatlmgndf vngqmfegff
      241 sriklprlrs kklkiskqht kmvgllywls tttadlalsq ilqtlkatrr eltakivset
      301 varyegftsp fvdklkngta mvltdaadgt fpewfstlyf cgilnpylin iaavhkvfhl
      361 tqidcfdles syscsltlrr alyglvlsvn sasdkmpsve efdrdgrnia rcfiepvldv
      421 pgygklpkle eiatltlekr kelffclfgv enipdqcpes lhgliavicf wlknsrvkan
      481 enllrsfllg ivlmkllprt klssrkasek lkflkngctv rsregigdil fgvdeqlnqd
      541 ilcrvnsnfs kffirpthny akrfdselvy lvsqlqtcll farflnrlll epcknvefhk
      601 vlngtflynf tkeldgrvnp dlfiaeylgr gtvldtgfal lyrkihnllp gdsfrpiqsa
      661 vggkkkkkkr agahdvteeq ddslvmlsnr fegl
//
LOCUS       XP_077503581             518 aa            linear   INV 25-NOV-2025
DEFINITION  arylsulfatase B-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503581
VERSION     XP_077503581.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647455.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..518
                     /product="arylsulfatase B-like isoform X1"
                     /calculated_mol_wt=57290
     Region          39..448
                     /region_name="4-S"
                     /note="N-acetylgalactosamine 4-sulfatase, also called
                     arylsulftase B; cd16029"
                     /db_xref="CDD:293753"
     Site            order(47..48,85,139,141,232,290..291)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:293753"
     Site            order(47..48,85,139,141,232,291)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:293753"
     CDS             1..518
                     /gene="LOC144114020"
                     /coded_by="XM_077647455.1:14..1570"
                     /db_xref="GeneID:144114020"
ORIGIN      
        1 manrgsprws dspccpalwp laaavllalg asahdapqph ivfivaddvg wndvpwhnag
       61 lfspnlarla adgvileqyy aqpvctptra ailtgrypyk lgrqghvlnp leptglttsv
      121 pllaeelsrh gysthalgkw hlgycnwsyt ptmrgfdsfs gfylgsqdhf lhtasasiam
      181 egfdyranat pewdargrys sklladdtvr llaaqasedr plflylafqa vhaplqvppe
      241 yevpctkynn snrrllcgml naldeavgri ekallhhgyw nntlliftsd nggqilqggn
      301 nwplrgnknt lweggtrvpa fvagpllkkv gysnskivha vdwfptllgl agekrahadl
      361 dgvnqwqvls edahqvrqef vynlrrrahl agairggdyk filgdgghpn gwypepnsed
      421 vvgkedvrni sralynirnd peerhdlsss qpeiaarler rlrklanhma pednpnddpr
      481 gdprrwggly apgwctlhdn lglslatvsa tsdseerk
//
LOCUS       XP_077503582             515 aa            linear   INV 25-NOV-2025
DEFINITION  arylsulfatase B-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503582
VERSION     XP_077503582.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647456.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..515
                     /product="arylsulfatase B-like isoform X2"
                     /calculated_mol_wt=57024
     Region          39..448
                     /region_name="4-S"
                     /note="N-acetylgalactosamine 4-sulfatase, also called
                     arylsulftase B; cd16029"
                     /db_xref="CDD:293753"
     Site            order(47..48,85,139,141,232,290..291)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:293753"
     Site            order(47..48,85,139,141,232,291)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:293753"
     CDS             1..515
                     /gene="LOC144114020"
                     /coded_by="XM_077647456.1:14..1561"
                     /db_xref="GeneID:144114020"
ORIGIN      
        1 manrgsprws dspccpalwp laaavllalg asahdapqph ivfivaddvg wndvpwhnag
       61 lfspnlarla adgvileqyy aqpvctptra ailtgrypyk lgrqghvlnp leptglttsv
      121 pllaeelsrh gysthalgkw hlgycnwsyt ptmrgfdsfs gfylgsqdhf lhtasasiam
      181 egfdyranat pewdargrys sklladdtvr llaaqasedr plflylafqa vhaplqvppe
      241 yevpctkynn snrrllcgml naldeavgri ekallhhgyw nntlliftsd nggqilqggn
      301 nwplrgnknt lweggtrvpa fvagpllkkv gysnskivha vdwfptllgl agekrahadl
      361 dgvnqwqvls edahqvrqef vynlrrrahl agairggdyk filgdgghpn gwypepnsed
      421 vvgkedvrni sralynirnd peerhdlsss qpeiaarler rlrklanhma pednpnddpr
      481 gdprrwggly apgwctlhdn laraplgacg mrllr
//
LOCUS       XP_077503583            1036 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114021 [Amblyomma americanum].
ACCESSION   XP_077503583
VERSION     XP_077503583.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647457.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1036
                     /product="uncharacterized protein LOC144114021"
                     /calculated_mol_wt=96203
     CDS             1..1036
                     /gene="LOC144114021"
                     /coded_by="XM_077647457.1:126..3236"
                     /db_xref="GeneID:144114021"
ORIGIN      
        1 mniamrqlvs glvlaiicst akashfhggp pdvhqgldgl gglslplgaf ggpggpgpge
       61 lgphggnfeg pgpvglggeg paggiddkgg yggpeaaaas gyggfdalgg pnqvmgpnvg
      121 lehgghdgpa gaggfggleg pghggpgdfg gpgdfggpgg fggpggpggp eglagpggpg
      181 gpeglagpgg lggpeglggp ggpggpeglg gpygpdglyg pgglgahggl gflgglggsg
      241 gllnlgglgg iggqggpggh gahlgssgig gnggpdglgg eigafggphk lghggyggvg
      301 gflgglggla grgglekdgv plgigaaggr dklvggsgvn gygmkaggrg gyvgagvngg
      361 yggidskagg asrqsgggqe aaygvqkyka dggggysgsp glpreggrle ggygggelkg
      421 sgaggyggvs gfiggggafd gsyggqdlkg sgprgysgaa llngaggglg esygsqdlka
      481 ggangyggfs glngaggdfg ggyrsqgvkg gavnsyggla gangvgggfg pgyggegvke
      541 sgaggyggls gyngasggfg ggygnqnlkg gegsgyggls glngaggglg ggfgsqglke
      601 vgaggyggla glnglsgglg gaygsqnlkg ggaggygsls gfngagggig ggyvsqdlkg
      661 saagsygglv gfngvggglr efyesqylkg gaaggyggla gfngggtgla evyagqeykg
      721 sgaggyggqs ikgaggqggy tglpmlsyqy gglsggyvet igsakgaalg gsseygenkg
      781 qgrgetgrkg rssgnsgkrg kggknrkgke sndsddvsvs legdeklrqa qiirlarfaa
      841 fqaaeqmakm nqevgqsvvv lpevaaspag lgelgvnalg sgvgigglgf sgagdlgagg
      901 lgvgglglgg laaegaalng glshagdgpe vvgavptpvt vekgpfgkes lndpyhyyky
      961 tdsmptskdf kypllnlght fgpdvplshs vkhvdsgyvy ghphknvhgf gvglgiripf
     1021 lglgveyskk ikrlhp
//
LOCUS       XP_077503584             172 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114022 [Amblyomma americanum].
ACCESSION   XP_077503584
VERSION     XP_077503584.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647458.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..172
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..172
                     /product="uncharacterized protein LOC144114022"
                     /calculated_mol_wt=17371
     CDS             1..172
                     /gene="LOC144114022"
                     /coded_by="XM_077647458.1:47..565"
                     /db_xref="GeneID:144114022"
ORIGIN      
        1 mispsgsqgy iswhqrhgrn qsftrnllrn tsimntlvvf aaliacayag gfgyggfggy
       61 gyghghghav avpvktvsyv kkpvvsvgyv trpvvsyvkq pvatvshtvq pvvsvshalv
      121 saggygggyg gyggavavgg yggyglgygl gyggyglggy ggyggflkgg yh
//
LOCUS       XP_077503585             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114024 [Amblyomma americanum].
ACCESSION   XP_077503585
VERSION     XP_077503585.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647459.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144114024"
                     /calculated_mol_wt=13032
     CDS             1..133
                     /gene="LOC144114024"
                     /coded_by="XM_077647459.1:142..543"
                     /db_xref="GeneID:144114024"
ORIGIN      
        1 mntlvvfaal iacayaggfg fggyghgygh ghghavavpv ktvsyvkkpv vsvgyvtrpv
       61 vsyvkqpvat vshtvqpvvs vshaivstgg ygggyggygg avalggyggy glghglgygg
      121 yggyggfvkg gyh
//
LOCUS       XP_077503586             483 aa            linear   INV 25-NOV-2025
DEFINITION  protein disulfide-isomerase TMX3-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503586
VERSION     XP_077503586.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647460.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..483
                     /product="protein disulfide-isomerase TMX3-like isoform
                     X1"
                     /calculated_mol_wt=54230
     Region          25..125
                     /region_name="Thioredoxin_like"
                     /note="Protein Disulfide Oxidoreductases and Other
                     Proteins with a Thioredoxin fold; cl00388"
                     /db_xref="CDD:469754"
     Region          43..>382
                     /region_name="PTZ00102"
                     /note="disulphide isomerase; Provisional"
                     /db_xref="CDD:240266"
     CDS             1..483
                     /gene="LOC144114025"
                     /coded_by="XM_077647460.1:76..1527"
                     /db_xref="GeneID:144114025"
ORIGIN      
        1 myhsqlfmsl ligvlicvpv ayaskvlels drfldvrheg swlvkfyapw cghckmlepi
       61 wsqvaqslad tdirvsrvdc trftsvamey svkgfptvlf ikggktveyk gdrnreelae
      121 fgrrmdgppv hhltscddlk amrerhkvff iytdgekgkd dsstlkeanq sstsvssvll
      181 kmagsrcrta perktadrsg eavwvtvsgi aeaenftkma eefqpvnyff tvppscvgql
      241 genmtspasp avyvvkdntg flydessase qnmtfldwmn rerfptfqki tygnfhqilk
      301 tgkkivtavl eenqigkitm rmqefkdmle aialntrelf hehfvfgwvg tpeiansvam
      361 mhlpvpsllv iqpetyqyfl peghsreqpp spqavlelln tildgsavpy ggdsfpyrly
      421 rayfeaktal agmwrgnpvl tavlfglplg flsiicyttc ccdvleasdd deeadeashe
      481 kke
//
LOCUS       XP_077503587             436 aa            linear   INV 25-NOV-2025
DEFINITION  protein disulfide-isomerase TMX3-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503587
VERSION     XP_077503587.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647461.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..436
                     /product="protein disulfide-isomerase TMX3-like isoform
                     X2"
                     /calculated_mol_wt=49353
     Region          25..125
                     /region_name="Thioredoxin_like"
                     /note="Protein Disulfide Oxidoreductases and Other
                     Proteins with a Thioredoxin fold; cl00388"
                     /db_xref="CDD:469754"
     Region          43..>363
                     /region_name="ER_PDI_fam"
                     /note="protein disulfide isomerase, eukaryotic; TIGR01130"
                     /db_xref="CDD:273457"
     CDS             1..436
                     /gene="LOC144114025"
                     /coded_by="XM_077647461.1:76..1386"
                     /db_xref="GeneID:144114025"
ORIGIN      
        1 myhsqlfmsl ligvlicvpv ayaskvlels drfldvrheg swlvkfyapw cghckmlepi
       61 wsqvaqslad tdirvsrvdc trftsvamey svkgfptvlf ikggktveyk gdrnreelae
      121 fgrrmdgppv hhltscddlk amrerhkvff iytdgekgkd dsstlkenft kmaeefqpvn
      181 yfftvppscv gqlgenmtsp aspavyvvkd ntgflydess aseqnmtfld wmnrerfptf
      241 qkitygnfhq ilktgkkivt avleenqigk itmrmqefkd mleaialntr elfhehfvfg
      301 wvgtpeians vammhlpvps llviqpetyq yflpeghsre qppspqavle llntildgsa
      361 vpyggdsfpy rlyrayfeak talagmwrgn pvltavlfgl plgflsiicy ttcccdvlea
      421 sdddeeadea shekke
//
LOCUS       XP_077503589             285 aa            linear   INV 25-NOV-2025
DEFINITION  ganglioside induced differentiation associated protein 1 isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077503589
VERSION     XP_077503589.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647463.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..285
                     /product="ganglioside induced differentiation associated
                     protein 1 isoform X1"
                     /calculated_mol_wt=32623
     Region          8..281
                     /region_name="GstA"
                     /note="Glutathione S-transferase [Posttranslational
                     modification, protein turnover, chaperones]; COG0625"
                     /db_xref="CDD:440390"
     CDS             1..285
                     /gene="Gdap1"
                     /coded_by="XM_077647463.1:165..1022"
                     /db_xref="GeneID:144114026"
ORIGIN      
        1 mpytneqiil ffypntigsr kmllvlytrg isfmprvmdv lhgeqmepwf ldmsplgelp
       61 vlkhgdsift dvdqmmlyid qicpstdpvg klfpdrgtel gnmveslvkd lskinipliv
      121 ygtlfhpema vssgmtddeq kkkatmvkqg kaqivelmer npdyvssyve kqraydtflg
      181 laqsenavsk eleeverimd smeellntrk isgtttpdat qtwlfsiyvs aadiylmvll
      241 havykaglwd ryfektqkkh knltryfkrm rhdpliskal pglik
//
LOCUS       XP_077503590             277 aa            linear   INV 25-NOV-2025
DEFINITION  ganglioside induced differentiation associated protein 1 isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077503590
VERSION     XP_077503590.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647464.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..277
                     /product="ganglioside induced differentiation associated
                     protein 1 isoform X2"
                     /calculated_mol_wt=30814
     Region          8..208
                     /region_name="GstA"
                     /note="Glutathione S-transferase [Posttranslational
                     modification, protein turnover, chaperones]; COG0625"
                     /db_xref="CDD:440390"
     CDS             1..277
                     /gene="Gdap1"
                     /coded_by="XM_077647464.1:177..1010"
                     /db_xref="GeneID:144114026"
ORIGIN      
        1 mpytneqiil ffypntigsr kmllvlytrg isfmprvmdv lhgeqmepwf ldmsplgelp
       61 vlkhgdsift dvdqmmlyid qicpstdpvg klfpdrgtel gnmveslvkd lskinipliv
      121 ygtlfhpema vssgmtddeq kkkanatqtw lfsiyvsaad iylmvllhav ykaglwdryf
      181 ektqkkhknl tryfkrmrhd pliskalpgl inalagpeae dqvpdggdad ppaavaaesa
      241 aaeraaaegp trarglmcta crvlrvrfaa stlhcsr
//
LOCUS       XP_077503591             350 aa            linear   INV 25-NOV-2025
DEFINITION  ganglioside induced differentiation associated protein 1 isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077503591
VERSION     XP_077503591.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647465.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..350
                     /product="ganglioside induced differentiation associated
                     protein 1 isoform X3"
                     /calculated_mol_wt=39125
     Region          8..281
                     /region_name="GstA"
                     /note="Glutathione S-transferase [Posttranslational
                     modification, protein turnover, chaperones]; COG0625"
                     /db_xref="CDD:440390"
     CDS             1..350
                     /gene="Gdap1"
                     /coded_by="XM_077647465.1:165..1217"
                     /db_xref="GeneID:144114026"
ORIGIN      
        1 mpytneqiil ffypntigsr kmllvlytrg isfmprvmdv lhgeqmepwf ldmsplgelp
       61 vlkhgdsift dvdqmmlyid qicpstdpvg klfpdrgtel gnmveslvkd lskinipliv
      121 ygtlfhpema vssgmtddeq kkkatmvkqg kaqivelmer npdyvssyve kqraydtflg
      181 laqsenavsk eleeverimd smeellntrk isgtttpdat qtwlfsiyvs aadiylmvll
      241 havykaglwd ryfektqkkh knltryfkrm rhdpliskal pglinalagp eaedqvpdgg
      301 dadppaavaa esaaaeraaa egptrarglm ctacrvlrvr faastlhcsr
//
LOCUS       XP_077503592             121 aa            linear   INV 25-NOV-2025
DEFINITION  U-scoloptoxin(18)-Er1a-like [Amblyomma americanum].
ACCESSION   XP_077503592
VERSION     XP_077503592.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647466.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..121
                     /product="U-scoloptoxin(18)-Er1a-like"
                     /calculated_mol_wt=12483
     CDS             1..121
                     /gene="LOC144114027"
                     /coded_by="XM_077647466.1:126..491"
                     /db_xref="GeneID:144114027"
ORIGIN      
        1 mrplvlaslc lvalaglsra dddddhvqqv atgprrsglh dgdschnnde cvskcclkvf
       61 dgdgsgspgh chaaagpgeh csleqvkgga nvnhcpcrvg icgddnicpn edsspeddhd
      121 h
//
LOCUS       XP_077503593             222 aa            linear   INV 25-NOV-2025
DEFINITION  dual specificity protein phosphatase 22-like [Amblyomma
            americanum].
ACCESSION   XP_077503593
VERSION     XP_077503593.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647467.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..222
                     /product="dual specificity protein phosphatase 22-like"
                     /calculated_mol_wt=24876
     Region          5..140
                     /region_name="DSP_DUSP22_15"
                     /note="dual specificity phosphatase domain of dual
                     specificity protein phosphatase 22, 15, and similar
                     proteins; cd14519"
                     /db_xref="CDD:350369"
     CDS             1..222
                     /gene="LOC144114028"
                     /coded_by="XM_077647467.1:240..908"
                     /db_xref="GeneID:144114028"
ORIGIN      
        1 mgngmnkvlp gvyignfrds kdpeqlqann ithiisihdt arklhedkey lciqasdtpg
       61 qnlsqffsqa ndfihsarme ggnvlihcla gvsrsvtiav ayvmsvtsln wrealkavrg
      121 arsianpnfg fqkqlseyec kklheerkrl rlkfpresfc edeqecrkll vayhnsvrpl
      181 epspsicrra pqgltpppsp rrrrhrspsp sagpstsyar pp
//
LOCUS       XP_077503594             160 aa            linear   INV 25-NOV-2025
DEFINITION  transmembrane protein 42 [Amblyomma americanum].
ACCESSION   XP_077503594
VERSION     XP_077503594.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647468.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..160
                     /product="transmembrane protein 42"
                     /calculated_mol_wt=17122
     Region          <65..138
                     /region_name="TMEM234"
                     /note="Putative transmembrane family 234; cl46810"
                     /db_xref="CDD:481150"
     CDS             1..160
                     /gene="LOC144114029"
                     /coded_by="XM_077647468.1:112..594"
                     /db_xref="GeneID:144114029"
ORIGIN      
        1 milpnemkgm tlsllsglla alaslcgkfs maggetssac qavfghwlsg asahylcgnl
       61 iavirvvffl lmigcnavmw tvftkalrlc tttleaavtn tasnffftai fgqtlfgeql
      121 tllwwlgtvm ilfglllmhq anvdskglht vrgssrykss
//
LOCUS       XP_077503595             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114030 [Amblyomma americanum].
ACCESSION   XP_077503595
VERSION     XP_077503595.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647469.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144114030"
                     /calculated_mol_wt=15173
     CDS             1..141
                     /gene="LOC144114030"
                     /coded_by="XM_077647469.1:303..728"
                     /db_xref="GeneID:144114030"
ORIGIN      
        1 mifrtglpaw inllfiwtpv hahiyednlg gqisllgplv cfavfiaica cfcrecrrre
       61 amlatttavp adpfhstgpv pygpavagar papmaqpshp pysppysqpp apsqsqpppp
      121 gflgypygsm pqdppppyey k
//
LOCUS       XP_077503596             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114030 [Amblyomma americanum].
ACCESSION   XP_077503596
VERSION     XP_077503596.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647470.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144114030"
                     /calculated_mol_wt=15173
     CDS             1..141
                     /gene="LOC144114030"
                     /coded_by="XM_077647470.1:362..787"
                     /db_xref="GeneID:144114030"
ORIGIN      
        1 mifrtglpaw inllfiwtpv hahiyednlg gqisllgplv cfavfiaica cfcrecrrre
       61 amlatttavp adpfhstgpv pygpavagar papmaqpshp pysppysqpp apsqsqpppp
      121 gflgypygsm pqdppppyey k
//
LOCUS       XP_077503597             603 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114032 [Amblyomma americanum].
ACCESSION   XP_077503597
VERSION     XP_077503597.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647471.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..603
                     /product="uncharacterized protein LOC144114032"
                     /calculated_mol_wt=67265
     CDS             1..603
                     /gene="LOC144114032"
                     /coded_by="XM_077647471.1:22..1833"
                     /db_xref="GeneID:144114032"
ORIGIN      
        1 mstesrarkf rrtrkrrvdv slqlpacaea gadplseqcs qlqrdvcvte rfvldtedvp
       61 tpdptsqdae agtssvfvdi ssdqdlcpqv dgrfdyeadh eradedvatf rhqlqtwave
      121 sgsshaavts llkvlrshkc fsslpssara llqtpkksne lsevsegqyc hfgveagire
      181 ilgqcrdlpp elkltfhidg lplskssrgq fwtilgrisn lkhaapfvts vffgdskprd
      241 aneflysfve elnillstgi aveaftipvk lkaivcdapa kafvlrvknh tgyysctkct
      301 vkgayiegrv cfpnisselr tdcsfrqrsq eqyhtgssiv eqlpidivrd vpldymhlvc
      361 lgvvhkllvl wfrgpkairl gskvrielsv rnvkaapfvp celnrkprsl tdldrwkate
      421 frffflyggp vllssllpsq myenflalhv aitilsmpng tksevqyagq llnhfvkgft
      481 kisgkqhash nvhglchlas dvehlgplds wsafpfenhm falkrllrkp erpleqlfnr
      541 lsqqkavlke aqqmpnfpll tehesgplia pcqgpqfkkv klpgsvvlap gkrtaaafck
      601 mdl
//
LOCUS       XP_077503598            3279 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114035 [Amblyomma americanum].
ACCESSION   XP_077503598
VERSION     XP_077503598.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647472.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..3279
                     /product="uncharacterized protein LOC144114035"
                     /calculated_mol_wt=356090
     Region          45..>122
                     /region_name="PLN03124"
                     /note="poly [ADP-ribose] polymerase; Provisional"
                     /db_xref="CDD:215591"
     Region          92..123
                     /region_name="SAP"
                     /note="SAP domain; pfam02037"
                     /db_xref="CDD:460424"
     Region          639..>766
                     /region_name="PRK11907"
                     /note="bifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase"
                     /db_xref="CDD:237019"
     Region          <2637..2893
                     /region_name="CDC27"
                     /note="DNA polymerase subunit Cdc27; pfam09507"
                     /db_xref="CDD:462819"
     Region          2959..3045
                     /region_name="RRM_ACINU"
                     /note="RNA recognition motif (RRM) found in apoptotic
                     chromatin condensation inducer in the nucleus (acinus) and
                     similar proteins; cd12432"
                     /db_xref="CDD:409866"
     Region          <2962..3143
                     /region_name="PABP-1234"
                     /note="polyadenylate binding protein, human types 1, 2, 3,
                     4 family; TIGR01628"
                     /db_xref="CDD:130689"
     Region          3146..3222
                     /region_name="RSB_motif"
                     /note="RNSP1-SAP18 binding (RSB) motif; pfam16294"
                     /db_xref="CDD:465085"
     CDS             1..3279
                     /gene="LOC144114035"
                     /coded_by="XM_077647472.1:16..9855"
                     /db_xref="GeneID:144114035"
ORIGIN      
        1 mlckiestce lkslqvhqcy edetdrrldn qhmpighdsd ghgmldssar gdarsnesrq
       61 rkraldqasv grrqqrrkma dksevgldgk tlhslrvvdl rsalekrglp ksgskkdlve
      121 rlklqlkiek lheesvkaed rvpnldlqde lagqndfvrq ylaaqqqtya mqlkerrlae
      181 kqaccsapeq mdseettsrp vvfsstgdee gihqeswedp cegrtcpssi dpggaaghsr
      241 tqskppveaa dppvetcrsr lrhrrpsppg svkrgrikpa glgepnqsaq pepnissgqn
      301 epsldikvqr vvrsprkgav apaispppie ghygqrypsr rkrqqsasee sakalqrpde
      361 htvititgee vehhvaihpa tpstvqhdrk eyarekrarl arqpkelgig hrtrratntt
      421 patpvtlskd ttneasslty csekssnrpt spqcsssrva elnyqgtsnl vqcpvvegse
      481 ergkplrmli trtgttksgi scsrvnmqse llsenaakah pntlftqvpc dalsgstlqs
      541 svtkdikqdq kitpsssslp saqvaddlhe tlnklhitte atdckenisv qrgalpmqet
      601 edivpnsael apndetliyg stfrkctint gkvaafqkke svqltvaatq evevmaaerd
      661 lnasptspse ghnvictdsi tadtstifar psprksptat emtsgvthtn svlkndsaie
      721 caadtkdtat ssvekecihp aaitaqeael avlqqeelnv spisadggna tgtgnitadt
      781 stspsrpsvr rsltaaettn avqsilqtte eilvrsehch dvldaepnli dedevgsatd
      841 sllkemsiga edgndlqftt tcletpeqvn tddmsmtgre aeeaavaass stttalygsd
      901 ssgnrsnaie dtktpkspdn tlcfqrepgp yktpscvppt gkientsmph kdelfgqerp
      961 pevsllrarn lvqeaevkkf sylqssdatg fvleanates csslkestsq qnaseesise
     1021 ksmstasatd slvvidsakl fedtsrmvss gasvhtekrk pmggtdcleh aactledqgl
     1081 ppaqkakeqg tsaksslldt paalptmaes kvclpegqqa ieqfdtgwtq krklrsdcpa
     1141 sasesrtale aiqnispfkv evglssnqpp sdypnadtac gttafslkgl qemhnlcada
     1201 akkdapleir satlhlnnte vtnseklssd daaksavaae tslhdedrlc hsggkqpakn
     1261 aeavvkfpct dthvtaryeg cstcateprk dtrvdtpala leqsallcnk qdahtdtslh
     1321 hgindqklpm eadavevpsp sgsesiesng rcfnnskeps slkkkkdlne ciqestaete
     1381 aaseafpsvs tgdlqvkded slessklpec kedvgheipe kaavveegae skfcnvvgts
     1441 ddkypdksgi cslalpqdte kqgkipreat tldhkillkq ekpkdktaia tynssheadh
     1501 arseytqild kydpefspal ahnrdayvkl dnlhctsnts vsdravtssm pmittsvaqv
     1561 dlsaecsasv lydetttphe sdagfrvaeh qqqcqgtssf gscdsttesy tasqqsrats
     1621 psqikqlvdt cnstnavlrt seiaensgdi vpapdstgie gsplqhagfa dscanavspa
     1681 clhsassiqg tnrptvditp csqavesaap cqfspphtvt nmprsatavq yaqedsqvlt
     1741 tpdrgavpkq esvaasvnsn egtvhnsple pqvgfagalg ncgavgstfq eenlssakks
     1801 nlcldednfa stgqkvpvit evkedlkrsl erdkqsplpv hniscesyaa klkeaasdga
     1861 kevffgvvsd eheydgkgik ceptantqmn irhsvkdnas thspkvttts ddgttneppl
     1921 ereesdpfqa vkehavvnss enkqvksngs hlstaacehq vftepvimvn tvheiaapsq
     1981 dllqrsvdsf lpehsknlka sgqesirece ntkdctfqil ssklismsne etmcatgfsn
     2041 tavdtsqair eavkigiplg nacknddasa hlhsavpede nrceeietta evsqlmeikh
     2101 shrnpmnvmt tgeatfglct edctgfhsit kacssqprlt lrhlaedelp epskieasrs
     2161 afvlkngdsq rtagelscad ipddkicles pprtqpslat gavqrilase calnlqhedv
     2221 sitkqvvdkc isktadsdft shehsctevt nssvdnvsak msstdhcavl taedsahgir
     2281 kmrdtecetg vpsvntstgi hsgifcldse gareetgelk gcnlsapglr addmvvdaaa
     2341 trtmeirsrv epqeddassh tnaapssltg stetsacses tadrlsncgv yepcrqevsn
     2401 pfrqysgtlp tstcllqcdt getfknvlgl gdssrssssd tdsseseehq marlrgrgvr
     2461 kqattratke pseassdgeg neagnrrlrn grratpeepv vtrrssrnqk vaaegspked
     2521 sdaatepesp sgsppgsppg sppgsppgsp pgsppgsppg spqpeeeqel spvqeeeqqq
     2581 qqqqqqqeep eqeqhmgkka sssstsessd ssdgeeepvg rrglkgvlvs spgrhqpape
     2641 qpsspptsep vleidtkeqe dvnnlgapae gdnsqcdqsr dqsppkedis akenasedve
     2701 mkdeeekepa seasapkfkt rrlstaalkk dsedkeerpq rkrrwgstnv vlgpslsist
     2761 aalkdlipdi eeplskpkdp eeehvsasvg espvteieea sdepplklqv lddeptsedv
     2821 ppgddsteng kerkdddeep sqdeksatte qeisnddgps rserfsrdee rplgrdekav
     2881 kvdkplrese kperldthar eskpardkas pitapgapga erktlrvisd raplskrsga
     2941 gkdeadkrkl pspprnpksr vlfvrnlvrp ftlnqlkqll qefgdtvdse fwidkikskc
     3001 fvtyvteeda vkarealhnl rwplcnpkil hvdfsspeem trhkepptps pwptgaatge
     3061 raapafqrtv evdphvpggr evlrdprell rdpreprdnr esrepkdpre argslsgpps
     3121 rtdrgmlver qaagrrpgmp mrewdrdkvr qetpppppae rvpapsrsrp hsppekrerr
     3181 dkklakrkpe edtpaklldd lfrktkaspc iywlplteeq varkeeerkq rrqererrrq
     3241 qqqlqeeeer rkralsrdvt aktkqeskrs gsrspvrrr
//
LOCUS       XP_077503599             595 aa            linear   INV 25-NOV-2025
DEFINITION  polynucleotide kinase 3'-phosphatase [Amblyomma americanum].
ACCESSION   XP_077503599
VERSION     XP_077503599.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647473.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..595
                     /product="polynucleotide kinase 3'-phosphatase"
                     /calculated_mol_wt=67469
     Region          16..592
                     /region_name="PNK-3'Pase"
                     /note="polynucleotide 5'-kinase 3'-phosphatase; TIGR01663"
                     /db_xref="CDD:130724"
     CDS             1..595
                     /gene="PNKP"
                     /coded_by="XM_077647473.1:149..1936"
                     /db_xref="GeneID:144114036"
ORIGIN      
        1 mrysncmlrs ksatlpniil phnqdtvvgr srstqirnkr csrcqvklra dfdsgtievt
       61 qlgenscrvs grrlaaretv tvhngdqfdl laeelpyevv feksfdhsip ssathteslk
      121 slncgekhrs krktegedmp lpkkhtnada rdesdehvke vsqklkhmqn sfkerrnhek
      181 svkpnegats slctlppvnp vksgwvelrd hhvlmynsdg leqkskiaaf dldgtlittk
      241 sgkvfpvnsr dwrillpqce arlrslieeg yklviitnqr glakghshea dfkakvehil
      301 kklavpaqvy vsvghgfyrk papgiwrhle aegnggipvk leesfyvgda agrpanwepk
      361 rkkdfscsdr lfalnigiaf ytpeefflkr paakfelpaf dprqvpdlpl aevvattgpn
      421 rskflngddl saghtevvvl vgypasgksh fakeylvpkq yvhinrdmlk swqkcveece
      481 kalerdrsav idntnpdpes rkrfidiakk hgcecrcfvf dcslerakhn nqfreiklkg
      541 qphisvtdmv lyshrskfke pclsegffei vkinfvpkfs tpedeklyrs flkdk
//
LOCUS       XP_077503600             568 aa            linear   INV 25-NOV-2025
DEFINITION  VPS9 domain-containing protein 1-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503600
VERSION     XP_077503600.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647474.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..568
                     /product="VPS9 domain-containing protein 1-like isoform
                     X1"
                     /calculated_mol_wt=62978
     Region          479..567
                     /region_name="VPS9"
                     /note="Vacuolar sorting protein 9 (VPS9) domain;
                     pfam02204"
                     /db_xref="CDD:460489"
     CDS             1..568
                     /gene="LOC144114037"
                     /coded_by="XM_077647474.1:117..1823"
                     /db_xref="GeneID:144114037"
ORIGIN      
        1 msmrctalsi cvpsavcwsd sridasaihc rgapalkkdp lihckrkayv nylksldaia
       61 asllkdydah gvpetplpvn nakkvisvar qcldrvekil gaslnqfyae qekcnetysc
      121 ppaiveleap ctpeitggag lppvafvapr ilsrqlpqql nsdttsdaal lsnpmfdvas
      181 stfmetkakr rvlspmeqme qentavcrry qaliqravdp vakqnlrlel qrrlmdnlma
      241 grkkqaeylk dlqeqekkta emaarklqgs gvcvtaeekr qqelfarilq yeskrewpsa
      301 lqdmleshpk npkaarhivm hilgdskhpl sqwlakerva lqreiaeald nashlveama
      361 apreptsgss nrsvacahve svlqtalfll gltfeplkdp eaqevcysvl edhftwplwp
      421 hllatvrlen lsgeqrvatv msqlasasaq emhvspclsr spalgeavgm lrtvprlgps
      481 hklrvlvhit rlvcteplas eddhekklig addlipalsy ilvqskvpql yseylaleqi
      541 ldsrymlgee gyclasvlma fkyleslp
//
LOCUS       XP_077503601             547 aa            linear   INV 25-NOV-2025
DEFINITION  VPS9 domain-containing protein 1-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503601
VERSION     XP_077503601.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647475.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..547
                     /product="VPS9 domain-containing protein 1-like isoform
                     X2"
                     /calculated_mol_wt=60908
     Region          458..546
                     /region_name="VPS9"
                     /note="Vacuolar sorting protein 9 (VPS9) domain;
                     pfam02204"
                     /db_xref="CDD:460489"
     CDS             1..547
                     /gene="LOC144114037"
                     /coded_by="XM_077647475.1:228..1871"
                     /db_xref="GeneID:144114037"
ORIGIN      
        1 mcdrfslvlq lvsqalgqdd rneyekayvn ylksldaiaa sllkdydahg vpetplpvnn
       61 akkvisvarq cldrvekilg aslnqfyaeq ekcnetyscp paiveleapc tpeitggagl
      121 ppvafvapri lsrqlpqqln sdttsdaall snpmfdvass tfmetkakrr vlspmeqmeq
      181 entavcrryq aliqravdpv akqnlrlelq rrlmdnlmag rkkqaeylkd lqeqekktae
      241 maarklqgsg vcvtaeekrq qelfarilqy eskrewpsal qdmleshpkn pkaarhivmh
      301 ilgdskhpls qwlakerval qreiaealdn ashlveamaa preptsgssn rsvacahves
      361 vlqtalfllg ltfeplkdpe aqevcysvle dhftwplwph llatvrlenl sgeqrvatvm
      421 sqlasasaqe mhvspclsrs palgeavgml rtvprlgpsh klrvlvhitr lvcteplase
      481 ddhekkliga ddlipalsyi lvqskvpqly seylaleqil dsrymlgeeg yclasvlmaf
      541 kyleslp
//
LOCUS       XP_077503602             555 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114038 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503602
VERSION     XP_077503602.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647476.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..555
                     /product="uncharacterized protein LOC144114038 isoform X1"
                     /calculated_mol_wt=63156
     Region          221..>525
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     CDS             1..555
                     /gene="LOC144114038"
                     /coded_by="XM_077647476.1:88..1755"
                     /db_xref="GeneID:144114038"
ORIGIN      
        1 meegavyctp tltskkkrra qekllrspva callpaisll avpgaslepa rpamsgpspg
       61 ksprfpiavd ipipperrts ainivtnpsf dggleqapee alystiqpkn ecgsspgtpp
      121 pplppkqlev lytkpikpkk aksregsrgs kdfgdgkgdg pqlwklhisq esppkpsqss
      181 gcncqqlasl lqdnyamkhe lltykdriks lttklkqfeg isevmkdlql svervseent
      241 rlkqyekcit eaktteqekh dlfeqlkdaq sqykydvdhl sqqvsqlere kqelegalak
      301 lqqehdqlli stqnsvsfqf httamlevkr lleelkqkyq ndkenltlkv qlaeqerqel
      361 kqqlvqleee vgwlrsdhsa lsgayrravr qnhtlvthlr elqdddsemh sllssllvta
      421 hqvvserdsl eikneekeke cqrmlellgq hnvdidqlnk ylknmqdqte dekfkaklrr
      481 evegnlqeey eqqiahlhsk veqkqqeles vhrekrkler eleqlwqvfa hetelkalpg
      541 dtaqafalhq lasqn
//
LOCUS       XP_077503603             541 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114038 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503603
VERSION     XP_077503603.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647477.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..541
                     /product="uncharacterized protein LOC144114038 isoform X2"
                     /calculated_mol_wt=61563
     Region          <226..>540
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..541
                     /gene="LOC144114038"
                     /coded_by="XM_077647477.1:88..1713"
                     /db_xref="GeneID:144114038"
ORIGIN      
        1 meegavyctp tltskkkrra qekllrspva callpaisll avpgaslepa rpamsgpspg
       61 ksprfpiavd ipipperrts ainivtnpsf dggleqapee alystiqpkn ecgsspgtpp
      121 pplppkqlev lytkpikpkk aksregsrgs kdfgdgkgdg pqlwklhisq esppkpsqss
      181 gcncqqlasl lqdnyamkhe lltykdriks lttklkqfeg isevmkdlql svervseent
      241 rlkqyekcit eaktteqekh dlfeqlkdaq sqykydvdhl sqqvsqlere kqelegalak
      301 lqqehdqlli stqnsvsfqf httamlevkr lleelkqkyq ndkenltlkv qlaeqerqel
      361 kqqlvqleee vgwlrsdhsa lsgayrravr qnhtlvthlr elqdddsemh sllssllvtn
      421 eekekecqrm lellgqhnvd idqlnkylkn mqdqtedekf kaklrreveg nlqeeyeqqi
      481 ahlhskveqk qqelesvhre krklereleq lwqvfahete lkalpgdtaq afalhqlasq
      541 n
//
LOCUS       XP_077503604             537 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114038 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503604
VERSION     XP_077503604.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647478.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..537
                     /product="uncharacterized protein LOC144114038 isoform X3"
                     /calculated_mol_wt=60851
     Region          221..>514
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     CDS             1..537
                     /gene="LOC144114038"
                     /coded_by="XM_077647478.1:88..1701"
                     /db_xref="GeneID:144114038"
ORIGIN      
        1 meegavyctp tltskkkrra qekllrspva callpaisll avpgaslepa rpamsgpspg
       61 ksprfpiavd ipipperrts ainivtnpsf dggleqapee alystiqpkn ecgsspgtpp
      121 pplppkqlev lytkpikpkk aksregsrgs kdfgdgkgdg pqlwklhisq esppkpsqss
      181 gcncqqlasl lqdnyamkhe lltykdriks lttklkqfeg isevmkdlql svervseent
      241 rlkqyekcit eaktteqekh dlfeqlkdaq sqykydvdhl sqqvsqlere kqelegalak
      301 lqqehdqlli stqnsvsfqf httamlevkr lleelkqkyq ndkenltlkv qlaeqerqel
      361 kqqlvqleee vgwlrsdhsa lsgayrravr qnhtlvthlr elqdddsemh sllssllvta
      421 hqvvserdsl eikneekeke cqrmlellgq hnvdidqlnk ylknmqdqte dekfkaklrr
      481 evegnlqeey eqqiahlhsk veqkqqeles vhrekrykas agskaqftsq kggvqlt
//
LOCUS       XP_077503605             537 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114038 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503605
VERSION     XP_077503605.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647479.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..537
                     /product="uncharacterized protein LOC144114038 isoform X4"
                     /calculated_mol_wt=60728
     Region          221..>514
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     CDS             1..537
                     /gene="LOC144114038"
                     /coded_by="XM_077647479.1:88..1701"
                     /db_xref="GeneID:144114038"
ORIGIN      
        1 meegavyctp tltskkkrra qekllrspva callpaisll avpgaslepa rpamsgpspg
       61 ksprfpiavd ipipperrts ainivtnpsf dggleqapee alystiqpkn ecgsspgtpp
      121 pplppkqlev lytkpikpkk aksregsrgs kdfgdgkgdg pqlwklhisq esppkpsqss
      181 gcncqqlasl lqdnyamkhe lltykdriks lttklkqfeg isevmkdlql svervseent
      241 rlkqyekcit eaktteqekh dlfeqlkdaq sqykydvdhl sqqvsqlere kqelegalak
      301 lqqehdqlli stqnsvsfqf httamlevkr lleelkqkyq ndkenltlkv qlaeqerqel
      361 kqqlvqleee vgwlrsdhsa lsgayrravr qnhtlvthlr elqdddsemh sllssllvta
      421 hqvvserdsl eikneekeke cqrmlellgq hnvdidqlnk ylknmqdqte dekfkaklrr
      481 evegnlqeey eqqiahlhsk veqkqqeles vhrekrapig tsllagnats mskslsq
//
LOCUS       XP_077503606             519 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114038 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077503606
VERSION     XP_077503606.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647480.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..519
                     /product="uncharacterized protein LOC144114038 isoform X5"
                     /calculated_mol_wt=59090
     Region          221..>514
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     CDS             1..519
                     /gene="LOC144114038"
                     /coded_by="XM_077647480.1:88..1647"
                     /db_xref="GeneID:144114038"
ORIGIN      
        1 meegavyctp tltskkkrra qekllrspva callpaisll avpgaslepa rpamsgpspg
       61 ksprfpiavd ipipperrts ainivtnpsf dggleqapee alystiqpkn ecgsspgtpp
      121 pplppkqlev lytkpikpkk aksregsrgs kdfgdgkgdg pqlwklhisq esppkpsqss
      181 gcncqqlasl lqdnyamkhe lltykdriks lttklkqfeg isevmkdlql svervseent
      241 rlkqyekcit eaktteqekh dlfeqlkdaq sqykydvdhl sqqvsqlere kqelegalak
      301 lqqehdqlli stqnsvsfqf httamlevkr lleelkqkyq ndkenltlkv qlaeqerqel
      361 kqqlvqleee vgwlrsdhsa lsgayrravr qnhtlvthlr elqdddsemh sllssllvta
      421 hqvvserdsl eikneekeke cqrmlellgq hnvdidqlnk ylknmqdqte dekfkaklrr
      481 evegnlqeey eqqiahlhsk veqkqqeles vhrekrhlq
//
LOCUS       XP_077503607             516 aa            linear   INV 25-NOV-2025
DEFINITION  kelch domain-containing protein 4 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503607
VERSION     XP_077503607.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647481.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..516
                     /product="kelch domain-containing protein 4 isoform X1"
                     /calculated_mol_wt=57723
     Region          64..350
                     /region_name="NanM"
                     /note="N-acetylneuraminic acid mutarotase [Cell
                     wall/membrane/envelope biogenesis]; COG3055"
                     /db_xref="CDD:442289"
     Region          67..118
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          122..177
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          180..224
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          234..296
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          299..350
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     CDS             1..516
                     /gene="LOC144114040"
                     /coded_by="XM_077647481.1:126..1676"
                     /db_xref="GeneID:144114040"
ORIGIN      
        1 mgkkdkkkgk gaektiakte kkavqklkke laakgeedie kmisqfveed rkrlavteel
       61 vgppscrsga tlcvhpekdq lllfggeyfn gqktfmynel yvynikknnw llvktpnlpp
      121 prcahqavmv pqgggqmwlf ggefasptrs qfyhykdlwv yhvvsrsweh vnapggpssr
      181 sghrmvhvgr qlllfggfhe svrdyryfnd lylfdldhrv wtkvecsnsg psprsgcqll
      241 pvaegkvlly ggysrekvkk dldqgkahtd mfllqadvhs pgrwkwstvk qsgcrpsprs
      301 gmsvavqpqg crayffggvq dqeeeetlvg hffndllcld mdrgfwrpvt lrasgeksda
      361 qlgdggtkee gphedlhklh idepvvtsdd gvftvkigls kekttdamsq eaatseapke
      421 sfipvprmgs claikhgify lyggmfednd rqftlsdmya ldlhkldkwk vlvpldpqmq
      481 ewlgsddsds esedmevdee gddesedgga casags
//
LOCUS       XP_077503608             421 aa            linear   INV 25-NOV-2025
DEFINITION  kelch domain-containing protein 4 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503608
VERSION     XP_077503608.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647482.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..421
                     /product="kelch domain-containing protein 4 isoform X2"
                     /calculated_mol_wt=47144
     Region          3..255
                     /region_name="NanM"
                     /note="N-acetylneuraminic acid mutarotase [Cell
                     wall/membrane/envelope biogenesis]; COG3055"
                     /db_xref="CDD:442289"
     Region          27..82
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          85..129
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          139..201
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          204..255
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     CDS             1..421
                     /gene="LOC144114040"
                     /coded_by="XM_077647482.1:111..1376"
                     /db_xref="GeneID:144114040"
ORIGIN      
        1 mynelyvyni kknnwllvkt pnlppprcah qavmvpqggg qmwlfggefa sptrsqfyhy
       61 kdlwvyhvvs rswehvnapg gpssrsghrm vhvgrqlllf ggfhesvrdy ryfndlylfd
      121 ldhrvwtkve csnsgpsprs gcqllpvaeg kvllyggysr ekvkkdldqg kahtdmfllq
      181 advhspgrwk wstvkqsgcr psprsgmsva vqpqgcrayf fggvqdqeee etlvghffnd
      241 llcldmdrgf wrpvtlrasg eksdaqlgdg gtkeegphed lhklhidepv vtsddgvftv
      301 kiglskektt damsqeaats eapkesfipv prmgsclaik hgifylyggm fedndrqftl
      361 sdmyaldlhk ldkwkvlvpl dpqmqewlgs ddsdsesedm evdeegddes edggacasag
      421 s
//
LOCUS       XP_077503609             510 aa            linear   INV 25-NOV-2025
DEFINITION  intraflagellar transport protein 74 homolog isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503609
VERSION     XP_077503609.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647483.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..510
                     /product="intraflagellar transport protein 74 homolog
                     isoform X1"
                     /calculated_mol_wt=58756
     Region          <91..>377
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          <269..>502
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..510
                     /gene="LOC144114041"
                     /coded_by="XM_077647483.1:558..2090"
                     /db_xref="GeneID:144114041"
ORIGIN      
        1 mdsrpttsts nrppspsmrs ftaatqrssl lgsvsrlsgr pstrtgppta mlsqmnildr
       61 pvtqhglvap ktglkssqrh vqdktyfigl lrakmaelna etnrlqrene slaqeqatys
      121 syekkaeala aelkelqgem adynllvdkl ntdadmedvk eeyrelksqn dqeansvell
      181 feqrqdrerq lrqleeeieq ernmadniia amqpelrhry aeqkelnqsl vdqldklqqq
      241 lqglqlqksa leedislssv kqeavklyer lrelgekkaa leeeqkqrgt paqererlla
      301 qvkednsels smerqasear erlgklqeel aqldqeleeh ngerrakyre lrkreetmda
      361 flesfegarh dehkrqeklq veivqllesc srslphflal psqeemavlk ddlifkenem
      421 ekskmtlsal gmeqkklavd lqkieqlesk vqqeldtlrq kikqmeedmv vfsdidklrs
      481 ssehkkqeli aekealqikk gptrktgrik
//
LOCUS       XP_077503610             506 aa            linear   INV 25-NOV-2025
DEFINITION  intraflagellar transport protein 74 homolog isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503610
VERSION     XP_077503610.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647484.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..506
                     /product="intraflagellar transport protein 74 homolog
                     isoform X2"
                     /calculated_mol_wt=58329
     Region          <91..>377
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          <269..>502
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..506
                     /gene="LOC144114041"
                     /coded_by="XM_077647484.1:558..2078"
                     /db_xref="GeneID:144114041"
ORIGIN      
        1 mdsrpttsts nrppspsmrs ftaatqrssl lgsvsrlsgr pstrtgppta mlsqmnildr
       61 pvtqhglvap ktglkssqrh vqdktyfigl lrakmaelna etnrlqrene slaqeqatys
      121 syekkaeala aelkelqgem adynllvdkl ntdadmedvk eeyrelksqn dqeansvell
      181 feqrqdrerq lrqleeeieq ernmadniia amqpelrhry aeqkelnqsl vdqldklqqq
      241 lqglqlqksa leedislssv kqeavklyer lrelgekkaa leeeqkqrgt paqererlla
      301 qvkednsels smerqasear erlgklqeel aqldqeleeh ngerrakyre lrkreetmda
      361 flesfegarh dehkrqeklq veivqllesc srslphflal psqeemavlk ddlifkenem
      421 ekskmtlsal gmeqkklavd lqkieqlesk vqqeldtlrq kikqmeedmv vfsdidklrs
      481 ssehkkqeli aekealqikk gptrkq
//
LOCUS       XP_077503611             588 aa            linear   INV 25-NOV-2025
DEFINITION  intraflagellar transport protein 74 homolog isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503611
VERSION     XP_077503611.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647485.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..588
                     /product="intraflagellar transport protein 74 homolog
                     isoform X3"
                     /calculated_mol_wt=67939
     Region          <88..>180
                     /region_name="46"
                     /note="endonuclease subunit; Provisional; PHA02562"
                     /db_xref="CDD:222878"
     Region          <162..>388
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          <293..>526
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..588
                     /gene="LOC144114041"
                     /coded_by="XM_077647485.1:558..2324"
                     /db_xref="GeneID:144114041"
ORIGIN      
        1 mdsrpttsts nrppspsmrs ftaatqrssl lgsvsrlsgr pstrtgppta mlsqmnildr
       61 pvtqhglvap ktglkssqrh vqdktyfigl lrakmaelna etnrlqrene slaqeqatys
      121 syekkaeala aelkelqgem adynllvdkl ntdadmedvk eeyrelksqn dqeansvell
      181 feqrqdrerq lrqleeeieq ernmadniia amqpelrhry aeqkelnqsl vdqldklqqq
      241 lqglqlqksa leedislssv kqeavklyer lrelgekkaa leeeqkqrgt paqererlla
      301 qvkednsels smerqasear erlgklqeel aqldqeleeh ngerrakyre lrkreetmda
      361 flesfegarh dehkrqeklq veivqllesc srslphflal psqeemavlk ddlifkenem
      421 ekskmtlsal gmeqkklavd lqkieqlesk vqqeldtlrq kikqmeedmv vfsdidklrs
      481 ssehkkqeli aekealqikk gptrkvveel qaqydearav lneeegytrl vnlerrwqhh
      541 eqnnfaaqey veaklaeyny hpirqnvqal leaynaalqe sltkasys
//
LOCUS       XP_077503612             571 aa            linear   INV 25-NOV-2025
DEFINITION  intraflagellar transport protein 74 homolog isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503612
VERSION     XP_077503612.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647486.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..571
                     /product="intraflagellar transport protein 74 homolog
                     isoform X4"
                     /calculated_mol_wt=66016
     Region          <88..>180
                     /region_name="46"
                     /note="endonuclease subunit; Provisional; PHA02562"
                     /db_xref="CDD:222878"
     Region          <162..>388
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          <293..>526
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..571
                     /gene="LOC144114041"
                     /coded_by="XM_077647486.1:558..2273"
                     /db_xref="GeneID:144114041"
ORIGIN      
        1 mdsrpttsts nrppspsmrs ftaatqrssl lgsvsrlsgr pstrtgppta mlsqmnildr
       61 pvtqhglvap ktglkssqrh vqdktyfigl lrakmaelna etnrlqrene slaqeqatys
      121 syekkaeala aelkelqgem adynllvdkl ntdadmedvk eeyrelksqn dqeansvell
      181 feqrqdrerq lrqleeeieq ernmadniia amqpelrhry aeqkelnqsl vdqldklqqq
      241 lqglqlqksa leedislssv kqeavklyer lrelgekkaa leeeqkqrgt paqererlla
      301 qvkednsels smerqasear erlgklqeel aqldqeleeh ngerrakyre lrkreetmda
      361 flesfegarh dehkrqeklq veivqllesc srslphflal psqeemavlk ddlifkenem
      421 ekskmtlsal gmeqkklavd lqkieqlesk vqqeldtlrq kikqmeedmv vfsdidklrs
      481 ssehkkqeli aekealqikk gptrkvveel qaqydearav lneeegytrl vnlerrwqhh
      541 eqnnfaaqdn eavlptkdlq fsqqlpnicr h
//
LOCUS       XP_077503613             167 aa            linear   INV 25-NOV-2025
DEFINITION  biogenesis of lysosome-related organelles complex 1, subunit 3
            [Amblyomma americanum].
ACCESSION   XP_077503613
VERSION     XP_077503613.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647487.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..167
                     /product="biogenesis of lysosome-related organelles
                     complex 1, subunit 3"
                     /calculated_mol_wt=18234
     Region          17..154
                     /region_name="BLOC1S3"
                     /note="Biogenesis of lysosome-related organelles complex 1
                     subunit 3; pfam15753"
                     /db_xref="CDD:464847"
     CDS             1..167
                     /gene="Blos3"
                     /coded_by="XM_077647487.1:188..691"
                     /db_xref="GeneID:144114042"
ORIGIN      
        1 maaptdaemi apveqlnvat sqqatvvcge asesddedls vatttvasdm svssvpesvt
       61 sserrsselg qhrtllhrkl rerngslrrn ciemvrqpyq yavrelggis qnlvrsqqll
      121 qevsaslrkl tnelfqiedk idtiqhcnai peitipssnl saspech
//
LOCUS       XP_077503614             634 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114045 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503614
VERSION     XP_077503614.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647488.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..634
                     /product="uncharacterized protein LOC144114045 isoform X1"
                     /calculated_mol_wt=69612
     Region          199..218
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(203,205,207,209..210,213..214,217,231,233,237..238,
                     241..242,245,259,261,263,265..266,269..270,273)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          210..235
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          <220..305
                     /region_name="SFP1"
                     /note="Putative transcriptional repressor regulating G2/M
                     transition [Transcription / Cell division and chromosome
                     partitioning]; COG5189"
                     /db_xref="CDD:227516"
     Region          226..246
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          254..274
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          282..299
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          295..317
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          310..330
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          322..344
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          338..356
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          367..393
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Region          394..414
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(394,397,410,414)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(399,401,403,405..406,409..410,413,427,429,433..434,
                     437..438,442,474,476,478,480..481,484..485,488)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          406..431
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          422..443
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          469..489
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..634
                     /gene="LOC144114045"
                     /coded_by="XM_077647488.1:450..2354"
                     /db_xref="GeneID:144114045"
ORIGIN      
        1 midrsgaalv tmpltpggsg dvhicgrcha tfsdiqsfla hkkqeelsqs adallpltsq
       61 htgttpssld kttsqfnaql giqqsllhdg qplhqegsgp paerghelha saahvltnir
      121 spgqaypapv qqasglpadv swpahptvns tsstgaavvy gvattaldvp sseqneeslk
      181 rhgqkqscae laslrcsqpg csftcryrkd lqrhsrihtg ekpykcpecs kefsrsdkma
      241 dhrrrhlger pfscgqcdya aadrwtlkmh qrvhiddkpy rcqmcpfagr hqnqllvhlr
      301 thtgdapfqc pecsarfris cdlqrhlrth tgerpyacrh csyqasvlsn lrsheramhs
      361 veqaahcnqc sftcsskrel rlhkashrea slhctecpyt cerrssltsh mrihdgtrpy
      421 qcqtcsyask hpanlrshik nkhskkpskt kngvanarqv ascsrtfqcs ecpeafvred
      481 slrshcrlhr kgilppaalv pppeepvqvt selltseqlp vvqsstnesi eqgidsacli
      541 stttstttft vctasdevqp lcqpsedatv ssqvleclvr dpvtveflpd kqllllapgq
      601 dstaasttnl fllesalqpa ywasppvatl ensa
//
LOCUS       XP_077503615             632 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114045 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503615
VERSION     XP_077503615.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647489.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..632
                     /product="uncharacterized protein LOC144114045 isoform X2"
                     /calculated_mol_wt=69355
     Region          199..218
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(203,205,207,209..210,213..214,217,231,233,237..238,
                     241..242,245,259,261,263,265..266,269..270,273)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          210..235
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          <220..305
                     /region_name="SFP1"
                     /note="Putative transcriptional repressor regulating G2/M
                     transition [Transcription / Cell division and chromosome
                     partitioning]; COG5189"
                     /db_xref="CDD:227516"
     Region          226..246
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          254..274
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          282..299
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          295..317
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          310..330
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          322..344
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          338..356
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          367..393
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Region          394..414
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(394,397,410,414)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(399,401,403,405..406,409..410,413,427,429,433..434,
                     437..438,442,474,476,478,480..481,484..485,488)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          406..431
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          422..443
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          469..489
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..632
                     /gene="LOC144114045"
                     /coded_by="XM_077647489.1:450..2348"
                     /db_xref="GeneID:144114045"
ORIGIN      
        1 midrsgaalv tmpltpggsg dvhicgrcha tfsdiqsfla hkkqeelsqs adallpltsq
       61 htgttpssld kttsqfnaql giqqsllhdg qplhqegsgp paerghelha saahvltnir
      121 spgqaypapv qqasglpadv swpahptvns tsstgaavvy gvattaldvp sseqneeslk
      181 rhgqkqscae laslrcsqpg csftcryrkd lqrhsrihtg ekpykcpecs kefsrsdkma
      241 dhrrrhlger pfscgqcdya aadrwtlkmh qrvhiddkpy rcqmcpfagr hqnqllvhlr
      301 thtgdapfqc pecsarfris cdlqrhlrth tgerpyacrh csyqasvlsn lrsheramhs
      361 veqaahcnqc sftcsskrel rlhkashrea slhctecpyt cerrssltsh mrihdgtrpy
      421 qcqtcsyask hpanlrshik nkhskkpskt kngvanarqv ascsrtfqcs ecpeafvred
      481 slrshcrlhr kgilppaalv pppeepvqvt selltslpvv qsstnesieq gidsaclist
      541 ttstttftvc tasdevqplc qpsedatvss qvleclvrdp vtveflpdkq llllapgqds
      601 taasttnlfl lesalqpayw asppvatlen sa
//
LOCUS       XP_077503616             286 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier family 66 member 2 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503616
VERSION     XP_077503616.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647490.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..286
                     /product="solute carrier family 66 member 2 isoform X1"
                     /calculated_mol_wt=31916
     Region          29..89
                     /region_name="PQ-loop"
                     /note="PQ loop repeat; pfam04193"
                     /db_xref="CDD:461220"
     Region          191..248
                     /region_name="PQ-loop"
                     /note="PQ loop repeat; pfam04193"
                     /db_xref="CDD:461220"
     CDS             1..286
                     /gene="LOC144114046"
                     /coded_by="XM_077647490.1:150..1010"
                     /db_xref="GeneID:144114046"
ORIGIN      
        1 mtsvagtglf gavhplgetl fmaspsqwls wgasaaiifg gvvpyipqyr eikrtknadg
       61 fstwvclall vantlrmlfw fgrpfelpll aqsvvmsscm llmlqlcvrt rslstivpqp
      121 kqrfteslse nppclqaadv airdltlwes pwghfwawtd flsyveflat fafcsgally
      181 llldvelvve avgfvallte allglpqfwr nlrnqstygm stqmvlmwtv gdvfktayfv
      241 mrlapmqfwv cgtlqvsldv aillqvvwyk yqpasakask hsshis
//
LOCUS       XP_077503617             286 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier family 66 member 2 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503617
VERSION     XP_077503617.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647491.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..286
                     /product="solute carrier family 66 member 2 isoform X1"
                     /calculated_mol_wt=31916
     Region          29..89
                     /region_name="PQ-loop"
                     /note="PQ loop repeat; pfam04193"
                     /db_xref="CDD:461220"
     Region          191..248
                     /region_name="PQ-loop"
                     /note="PQ loop repeat; pfam04193"
                     /db_xref="CDD:461220"
     CDS             1..286
                     /gene="LOC144114046"
                     /coded_by="XM_077647491.1:385..1245"
                     /db_xref="GeneID:144114046"
ORIGIN      
        1 mtsvagtglf gavhplgetl fmaspsqwls wgasaaiifg gvvpyipqyr eikrtknadg
       61 fstwvclall vantlrmlfw fgrpfelpll aqsvvmsscm llmlqlcvrt rslstivpqp
      121 kqrfteslse nppclqaadv airdltlwes pwghfwawtd flsyveflat fafcsgally
      181 llldvelvve avgfvallte allglpqfwr nlrnqstygm stqmvlmwtv gdvfktayfv
      241 mrlapmqfwv cgtlqvsldv aillqvvwyk yqpasakask hsshis
//
LOCUS       XP_077503618             263 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier family 66 member 2 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503618
VERSION     XP_077503618.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647492.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..263
                     /product="solute carrier family 66 member 2 isoform X2"
                     /calculated_mol_wt=29392
     Region          29..89
                     /region_name="PQ-loop"
                     /note="PQ loop repeat; pfam04193"
                     /db_xref="CDD:461220"
     Region          168..225
                     /region_name="PQ-loop"
                     /note="PQ loop repeat; pfam04193"
                     /db_xref="CDD:461220"
     CDS             1..263
                     /gene="LOC144114046"
                     /coded_by="XM_077647492.1:150..941"
                     /db_xref="GeneID:144114046"
ORIGIN      
        1 mtsvagtglf gavhplgetl fmaspsqwls wgasaaiifg gvvpyipqyr eikrtknadg
       61 fstwvclall vantlrmlfw fgrpfelpll aqsvvmsscm llmlqlcvrt rslstivpqp
      121 kqrftespwg hfwawtdfls yveflatfaf csgallylll dvelvveavg fvallteall
      181 glpqfwrnlr nqstygmstq mvlmwtvgdv fktayfvmrl apmqfwvcgt lqvsldvail
      241 lqvvwykyqp asakaskhss his
//
LOCUS       XP_077503619             263 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier family 66 member 2 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503619
VERSION     XP_077503619.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647493.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..263
                     /product="solute carrier family 66 member 2 isoform X2"
                     /calculated_mol_wt=29392
     Region          29..89
                     /region_name="PQ-loop"
                     /note="PQ loop repeat; pfam04193"
                     /db_xref="CDD:461220"
     Region          168..225
                     /region_name="PQ-loop"
                     /note="PQ loop repeat; pfam04193"
                     /db_xref="CDD:461220"
     CDS             1..263
                     /gene="LOC144114046"
                     /coded_by="XM_077647493.1:343..1134"
                     /db_xref="GeneID:144114046"
ORIGIN      
        1 mtsvagtglf gavhplgetl fmaspsqwls wgasaaiifg gvvpyipqyr eikrtknadg
       61 fstwvclall vantlrmlfw fgrpfelpll aqsvvmsscm llmlqlcvrt rslstivpqp
      121 kqrftespwg hfwawtdfls yveflatfaf csgallylll dvelvveavg fvallteall
      181 glpqfwrnlr nqstygmstq mvlmwtvgdv fktayfvmrl apmqfwvcgt lqvsldvail
      241 lqvvwykyqp asakaskhss his
//
LOCUS       XP_077503620             263 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier family 66 member 2 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503620
VERSION     XP_077503620.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647494.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..263
                     /product="solute carrier family 66 member 2 isoform X2"
                     /calculated_mol_wt=29392
     Region          29..89
                     /region_name="PQ-loop"
                     /note="PQ loop repeat; pfam04193"
                     /db_xref="CDD:461220"
     Region          168..225
                     /region_name="PQ-loop"
                     /note="PQ loop repeat; pfam04193"
                     /db_xref="CDD:461220"
     CDS             1..263
                     /gene="LOC144114046"
                     /coded_by="XM_077647494.1:385..1176"
                     /db_xref="GeneID:144114046"
ORIGIN      
        1 mtsvagtglf gavhplgetl fmaspsqwls wgasaaiifg gvvpyipqyr eikrtknadg
       61 fstwvclall vantlrmlfw fgrpfelpll aqsvvmsscm llmlqlcvrt rslstivpqp
      121 kqrftespwg hfwawtdfls yveflatfaf csgallylll dvelvveavg fvallteall
      181 glpqfwrnlr nqstygmstq mvlmwtvgdv fktayfvmrl apmqfwvcgt lqvsldvail
      241 lqvvwykyqp asakaskhss his
//
LOCUS       XP_077503621             257 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier family 66 member 2 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503621
VERSION     XP_077503621.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647495.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..257
                     /product="solute carrier family 66 member 2 isoform X3"
                     /calculated_mol_wt=28397
     Region          29..89
                     /region_name="PQ-loop"
                     /note="PQ loop repeat; pfam04193"
                     /db_xref="CDD:461220"
     CDS             1..257
                     /gene="LOC144114046"
                     /coded_by="XM_077647495.1:150..923"
                     /db_xref="GeneID:144114046"
ORIGIN      
        1 mtsvagtglf gavhplgetl fmaspsqwls wgasaaiifg gvvpyipqyr eikrtknadg
       61 fstwvclall vantlrmlfw fgrpfelpll aqsvvmsscm llmlqlcvrt rslstivpqp
      121 kqrfteslse nppclqaadv airdltlwes pwghfwawtd flsyveflat fafcsgally
      181 llldvelvve avgfvallte allglpqfwr nlrnqstygm rwcgtntsqh ppkhpstavi
      241 sadsaihmga vfmslql
//
LOCUS       XP_077503623             234 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier family 66 member 2 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503623
VERSION     XP_077503623.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647497.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..234
                     /product="solute carrier family 66 member 2 isoform X4"
                     /calculated_mol_wt=25873
     Region          29..89
                     /region_name="PQ-loop"
                     /note="PQ loop repeat; pfam04193"
                     /db_xref="CDD:461220"
     CDS             1..234
                     /gene="LOC144114046"
                     /coded_by="XM_077647497.1:150..854"
                     /db_xref="GeneID:144114046"
ORIGIN      
        1 mtsvagtglf gavhplgetl fmaspsqwls wgasaaiifg gvvpyipqyr eikrtknadg
       61 fstwvclall vantlrmlfw fgrpfelpll aqsvvmsscm llmlqlcvrt rslstivpqp
      121 kqrftespwg hfwawtdfls yveflatfaf csgallylll dvelvveavg fvallteall
      181 glpqfwrnlr nqstygmrwc gtntsqhppk hpstavisad saihmgavfm slql
//
LOCUS       XP_077503624            1190 aa            linear   INV 25-NOV-2025
DEFINITION  sal-like protein 1 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503624
VERSION     XP_077503624.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647498.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1190
                     /product="sal-like protein 1 isoform X1"
                     /calculated_mol_wt=126661
     Region          336..406
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          339..360
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          339..359
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(344,350,357,359,366,373)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          367..387
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          367..387
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          <385..558
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          612..>665
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          612..633
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          612..632
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(612,615,628,632)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(617,619,621,623..624,627..628,631,645,647,651..652,
                     655..656,659,677,679,681,683..684,687..688,691)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Site            order(617,623,630,632,639,646)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          624..649
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          640..660
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          640..660
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          670..692
                     /region_name="ZnF_C2H2"
                     /note="zinc finger; smart00355"
                     /db_xref="CDD:197676"
     Region          672..692
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          <732..>875
                     /region_name="PTZ00449"
                     /note="104 kDa microneme/rhoptry antigen; Provisional"
                     /db_xref="CDD:185628"
     Region          893..>943
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          893..914
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          893..913
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(898,904,911,913,920,927)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          919..941
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          921..941
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          921..941
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(921,924,937,941)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(926,928,930,932..933,936..937,940,1024,1026,
                     1030..1031,1034..1035,1038,1052,1054,1056,1058..1059,
                     1062..1063,1066)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1016..>1064
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          1019..1040
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          1019..1039
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1024,1030,1037,1039,1046,1053)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          1047..1067
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1047..1064
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     CDS             1..1190
                     /gene="LOC144114048"
                     /coded_by="XM_077647498.1:671..4243"
                     /db_xref="GeneID:144114048"
ORIGIN      
        1 msrrkqacps rhiaspasss lcvgnshnga vvhrssclpg qlplrkssgr ggsstggeee
       61 ssddehvcgr craefptldh flahkrccps vesmglpgst psrlscsddd aagrqsasda
      121 aevdeepsva arlpsthval ealqstkvav aqfaygssgg paeiaalhea lmalqqqqvv
      181 qlhiiqqlqa yvgaspalpv nltraadstp pppapppprt vpspeapggq thatppppnt
      241 vvdlatkesa apepadppaa esplgepmgg tllvappagp pppdepntle llqrhtekal
      301 qdtmsggsfl lnglgspdps rlrrkgdgrp deaylrhrcr fcgkvfgsds alqihirsht
      361 gerpfkcnvc gnrfstkgnl kvhfqrhrak yphvrmnphp vpehldkhfp pleppgdrsp
      421 tspsgatssp ppslqpalal qpaplgvlvk aaaprpedlv rpscsgalal ratrstspgs
      481 ertsnvttpm tsaeleserp lslkaepede addkvsdedr dscgvsgsad dsmpftsags
      541 flsgsfstlq ttadaavplh adpafyqdll pkpgsndnsw etlmevtrps etsklqqlvd
      601 niehklsdpn qcvichrvls crsalqmhyr thtgerpfrc kicgrafttk gnlkthmgvh
      661 rvkpplrvlh kcpvchkqft nslvlqqhvr mhgseslhat qsfqgtsqrp tpppktsssp
      721 ppssptspap eqkkeegrsp spqplmrpls perppsqlts laalenqvkg iktslqvpal
      781 pfgpfgmgls sgfgrfddqs lllgrtpetg qrspafspra gselstgder stpgapspsg
      841 gtsppvnggp ldltprtllg rpadsfsgrf faapplgglp fpgaapgrpn ttcqicfktf
      901 acnsaleihy rshtkerpfk csvcergfst kgnlkqhmlt hkirdlpatl faatpsagas
      961 gpspapspgr saspcseqrp gaspdesrtk rvepggpppp ppppqhqprr ppglprhvch
     1021 vcnkpfssss slqihmrtht gdrpfkcsvc grafttkgnl kvhmgthmwn ngssrrgrrm
     1081 sielpsllsg pkadfvpppp pphelfypyl gpaylngmlg pkaneisviq gasteprpgd
     1141 eaepqngawa wkmacnlcak icgsslelea hlkahhrkel evqspdnvav
//
LOCUS       XP_077503625            1172 aa            linear   INV 25-NOV-2025
DEFINITION  sal-like protein 1 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503625
VERSION     XP_077503625.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647499.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1172
                     /product="sal-like protein 1 isoform X2"
                     /calculated_mol_wt=124731
     Region          318..388
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          321..342
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          321..341
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(326,332,339,341,348,355)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          349..369
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          349..369
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          <367..540
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          594..>647
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          594..615
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          594..614
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(594,597,610,614)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(599,601,603,605..606,609..610,613,627,629,633..634,
                     637..638,641,659,661,663,665..666,669..670,673)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Site            order(599,605,612,614,621,628)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          606..631
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          622..642
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          622..642
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          652..674
                     /region_name="ZnF_C2H2"
                     /note="zinc finger; smart00355"
                     /db_xref="CDD:197676"
     Region          654..674
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          <714..>857
                     /region_name="PTZ00449"
                     /note="104 kDa microneme/rhoptry antigen; Provisional"
                     /db_xref="CDD:185628"
     Region          875..>925
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          875..896
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          875..895
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(880,886,893,895,902,909)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          901..923
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          903..923
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          903..923
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(903,906,919,923)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(908,910,912,914..915,918..919,922,1006,1008,
                     1012..1013,1016..1017,1020,1034,1036,1038,1040..1041,
                     1044..1045,1048)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          998..>1046
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          1001..1022
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          1001..1021
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1006,1012,1019,1021,1028,1035)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          1029..1049
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1029..1046
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     CDS             1..1172
                     /gene="LOC144114048"
                     /coded_by="XM_077647499.1:671..4189"
                     /db_xref="GeneID:144114048"
ORIGIN      
        1 msrrkqacps rhiaspasss lcvgnshngs grggsstgge eessddehvc grcraefptl
       61 dhflahkrcc psvesmglpg stpsrlscsd ddaagrqsas daaevdeeps vaarlpsthv
      121 alealqstkv avaqfaygss ggpaeiaalh ealmalqqqq vvqlhiiqql qayvgaspal
      181 pvnltraads tppppapppp rtvpspeapg gqthatpppp ntvvdlatke saapepadpp
      241 aaesplgepm ggtllvappa gppppdepnt lellqrhtek alqdtmsggs fllnglgspd
      301 psrlrrkgdg rpdeaylrhr crfcgkvfgs dsalqihirs htgerpfkcn vcgnrfstkg
      361 nlkvhfqrhr akyphvrmnp hpvpehldkh fppleppgdr sptspsgats spppslqpal
      421 alqpaplgvl vkaaaprped lvrpscsgal alratrstsp gsertsnvtt pmtsaelese
      481 rplslkaepe deaddkvsde drdscgvsgs addsmpftsa gsflsgsfst lqttadaavp
      541 lhadpafyqd llpkpgsndn swetlmevtr psetsklqql vdniehklsd pnqcvichrv
      601 lscrsalqmh yrthtgerpf rckicgraft tkgnlkthmg vhrvkpplrv lhkcpvchkq
      661 ftnslvlqqh vrmhgseslh atqsfqgtsq rptpppktss spppssptsp apeqkkeegr
      721 spspqplmrp lsperppsql tslaalenqv kgiktslqvp alpfgpfgmg lssgfgrfdd
      781 qslllgrtpe tgqrspafsp ragselstgd erstpgapsp sggtsppvng gpldltprtl
      841 lgrpadsfsg rffaapplgg lpfpgaapgr pnttcqicfk tfacnsalei hyrshtkerp
      901 fkcsvcergf stkgnlkqhm lthkirdlpa tlfaatpsag asgpspapsp grsaspcseq
      961 rpgaspdesr tkrvepggpp ppppppqhqp rrppglprhv chvcnkpfss ssslqihmrt
     1021 htgdrpfkcs vcgrafttkg nlkvhmgthm wnngssrrgr rmsielpsll sgpkadfvpp
     1081 pppphelfyp ylgpaylngm lgpkaneisv iqgasteprp gdeaepqnga wawkmacnlc
     1141 akicgsslel eahlkahhrk elevqspdnv av
//
LOCUS       XP_077503626            1023 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114049 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503626
VERSION     XP_077503626.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647500.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1023
                     /product="uncharacterized protein LOC144114049 isoform X1"
                     /calculated_mol_wt=109827
     Region          2..216
                     /region_name="BAR"
                     /note="The Bin/Amphiphysin/Rvs (BAR) domain, a
                     dimerization module that binds membranes and detects
                     membrane curvature; cl12013"
                     /db_xref="CDD:472257"
     Site            order(3,5..6,9..10,12..13,24..25,27..28,31..32,34..35,38,
                     63,66..67,164,167..168,170..171,174..175,177..178,
                     181..182,185..186,188..189,191..192,194..195)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153271"
     CDS             1..1023
                     /gene="LOC144114049"
                     /coded_by="XM_077647500.1:373..3444"
                     /db_xref="GeneID:144114049"
ORIGIN      
        1 mggtpiwedf lakasklhsq lkitasvsaa fldsfqkvad matntkgatk evgkaltrlc
       61 lrhrsveakl rsftasimeq lviplqdkle dwkkstlqld kdhskefkra rqeikkmstd
      121 tlrlqkkvkk gsgkwemqrs lecalqemsd rcllleeaek qavrralvee rarycllvac
      181 ltpvleeell mlqetshlqe ildslakltr npeclpsase qvitdlkgsq gtwtfqtppg
      241 spgpaggsts sslgsrkssm csissfnsss sgstkshsps hqlrcraasq qppvggplrl
      301 ssvssqdsgf tsqdtlflrp psprgtnmva qvsagdagms slpgcasssp sspfpttpsv
      361 tstwpnlqds lqferamnsv lrcqrphtis sayersghqa rplltaetfq plegdllsss
      421 nhsgsadtlt stsgdssdgl elaaqpapas garpkspqtp trgprppvpi rtssststap
      481 slpekpallk qaavatvgas dvrplgqvts qpiyanadel avaastlpgt espplppppi
      541 qdslqaissw epdgmtpmrr gsalqmheay ktyspsnssm ryeglsgsst lpakssfrrs
      601 sqppppppvr rtpsitstqg cspsspssqh iqevpsdrgc qsvgdsddss tsrakliqal
      661 narfskpspl eqaglrfslp sshtedscgl plpdppetsh fdpendskpf papppflepv
      721 endplldveg ehagalrkqv qpekkllrah tighserdal iqslnakfst pevtppsetk
      781 maarktlpce spsmacarsq rsgvgaamks gksppemdff ranleqtllk relkrdprts
      841 iaggttdsag enrrsrsvgp vpsgekknns aerrqflssl narlaeqrra slqpaqqlap
      901 svkpaqytqq mttfctgqtt vpvpaqyvhm tssqiqnvqv vqavpgtlpq ttavttrrgs
      961 lqpdvefglr knggraarvq swlagrgsvd ltacreslmd qirqgtvlkr vasqsdrstp
     1021 lll
//
LOCUS       XP_077503627             982 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114049 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503627
VERSION     XP_077503627.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647501.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..982
                     /product="uncharacterized protein LOC144114049 isoform X2"
                     /calculated_mol_wt=105407
     Region          <1..176
                     /region_name="BAR"
                     /note="The Bin/Amphiphysin/Rvs (BAR) domain, a
                     dimerization module that binds membranes and detects
                     membrane curvature; cl12013"
                     /db_xref="CDD:472257"
     CDS             1..982
                     /gene="LOC144114049"
                     /coded_by="XM_077647501.1:114..3062"
                     /db_xref="GeneID:144114049"
ORIGIN      
        1 matntkgatk evgkaltrlc lrhrsveakl rsftasimeq lviplqdkle dwkkstlqld
       61 kdhskefkra rqeikkmstd tlrlqkkvkk gsgkwemqrs lecalqemsd rcllleeaek
      121 qavrralvee rarycllvac ltpvleeell mlqetshlqe ildslakltr npeclpsase
      181 qvitdlkgsq gtwtfqtppg spgpaggsts sslgsrkssm csissfnsss sgstkshsps
      241 hqlrcraasq ppvggplrls svssqdsgft sqdtlflrpp sprgtnmvaq vsagdagmss
      301 lpgcasssps spfpttpsvt stwpnlqdsl qferamnsvl rcqrphtiss ayersghqar
      361 plltaetfqp legdllsssn hsgsadtlts tsgdssdgle laaqpapasg arpkspqtpt
      421 rgprppvpir tssststaps lpekpallkq aavatvgasd vrplgqvtsq piyanadela
      481 vaastlpgte spplppppiq dslqaisswe pdgmtpmrrg salqmheayk tyspsnssmr
      541 yeglsgsstl pakssfrrss qppppppvrr tpsitstqgc spsspssqhi qevpsdrgcq
      601 svgdsddsst srakliqaln arfskpsple qaglrfslps shtedscglp lpdppetshf
      661 dpendskpfp apppflepve ndplldvege hagalrkqvq pekkllraht ighserdali
      721 qslnakfstp evtppsetkm aarktlpces psmacarsqr sgvgaamksg ksppemdffr
      781 anleqtllkr elkrdprtsi aggttdsage nrrsrsvgpv psgekknnsa errqflssln
      841 arlaeqrras lqpaqqlaps vkpaqytqqm ttfctgqttv pvpaqyvhmt ssqiqnvqvv
      901 qavpgtlpqt tavttrrgsl qpdvefglrk nggraarvqs wlagrgsvdl tacreslmdq
      961 irqgtvlkrv asqsdrstpl ll
//
LOCUS       XP_077503628             193 aa            linear   INV 25-NOV-2025
DEFINITION  cilia- and flagella-associated protein 20-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077503628
VERSION     XP_077503628.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647502.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..193
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..193
                     /product="cilia- and flagella-associated protein 20-like
                     isoform X1"
                     /calculated_mol_wt=22620
     Region          1..185
                     /region_name="CFA20_dom"
                     /note="CFA20 domain; pfam05018"
                     /db_xref="CDD:461521"
     CDS             1..193
                     /gene="LOC144114051"
                     /coded_by="XM_077647502.1:162..743"
                     /db_xref="GeneID:144114051"
ORIGIN      
        1 mfkntfqsgf lsilysigsk plqiwdkkvr nghikritdn diqsfvleil gtnvsttfit
       61 cpadprktlg irlpylimii knmkkyftfe vqilddknvr rrfrasnfqs ttrvkpfict
      121 mpmrldegwn qiqfnladft rraygtgyve tlrmqihanc rirrvyfadr lysedelpae
      181 fklylpvqar akv
//
LOCUS       XP_077503629             160 aa            linear   INV 25-NOV-2025
DEFINITION  cilia- and flagella-associated protein 20-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077503629
VERSION     XP_077503629.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647503.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..160
                     /product="cilia- and flagella-associated protein 20-like
                     isoform X2"
                     /calculated_mol_wt=18576
     Region          1..155
                     /region_name="CFA20_dom"
                     /note="CFA20 domain; pfam05018"
                     /db_xref="CDD:461521"
     CDS             1..160
                     /gene="LOC144114051"
                     /coded_by="XM_077647503.1:162..644"
                     /db_xref="GeneID:144114051"
ORIGIN      
        1 mfkntfqsgf lsilysigsk plqiwdkkvr nghikritdn diqsfvleil gtnvsttfit
       61 cpadprktlg irlpylimii knmkkyftfe vqilddknvr rrfrasnfqs ttrvkpfict
      121 mpmrldegwn qiqfnladft rraygtgyve tlrmqsfaal
//
LOCUS       XP_077503630             640 aa            linear   INV 25-NOV-2025
DEFINITION  protein arginine N-methyltransferase 5 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503630
VERSION     XP_077503630.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647504.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..640
                     /product="protein arginine N-methyltransferase 5 isoform
                     X1"
                     /calculated_mol_wt=73179
     Region          30..293
                     /region_name="PRMT5_TIM"
                     /note="PRMT5 TIM barrel domain; pfam17285"
                     /db_xref="CDD:435840"
     Region          299..467
                     /region_name="PRMT5"
                     /note="PRMT5 arginine-N-methyltransferase; pfam05185"
                     /db_xref="CDD:428356"
     Region          470..638
                     /region_name="PRMT5_C"
                     /note="PRMT5 oligomerization domain; pfam17286"
                     /db_xref="CDD:435841"
     CDS             1..640
                     /gene="csul"
                     /coded_by="XM_077647504.1:64..1986"
                     /db_xref="GeneID:144114053"
ORIGIN      
        1 maaqqkvscg vdlqfsanlr qglifagnag fkfvavdish prarrefieh dvktnprpfa
       61 rsdlvlpsqd wnslvtlkis kwidvdspve sfrkaseevl nqemcyaahl gvpailihvr
      121 srrcvnlsrm lhnfifasht yqlmfqawil ipmepasqqs rqhlcslsge gnqcdnddnn
      181 nvddfsrded pwewwnmfrn vagpekrlgl alslpanipd esamrrwmge pvkcivists
      241 vfltnkkgfp vlskahqavl hqffklncql lldgrcrhkh mrfyyqyldh vfkarpqndp
      301 lsefakgfed ylqtplqplm dnleavtyev fekdpvkytq yqkaihlalv dkgeqvgkdt
      361 eiiimvvgag rgplvraalt aaelagqkvr lyaieknpna vitllsqqtd swkdrvtvvs
      421 cdmrdfdpse kadilvsell gsfgdnelsp ecldgaqrfl kddgisipck yssylsplqs
      481 qklyievaal rekdkhplss yevpyvvrlh nvtelaehqr lftfvhpnkd kthdnsryks
      541 lrfkvrdnsi lhgfagyfdt vlykdvtlsi cpdthspgmf swfpmffplk eplrlnkgst
      601 levhfwrcvt qrkvwyewlv tepeigtihn pcgrsyiigl
//
LOCUS       XP_077503631             636 aa            linear   INV 25-NOV-2025
DEFINITION  protein arginine N-methyltransferase 5 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503631
VERSION     XP_077503631.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647505.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..636
                     /product="protein arginine N-methyltransferase 5 isoform
                     X2"
                     /calculated_mol_wt=72659
     Region          30..289
                     /region_name="PRMT5_TIM"
                     /note="PRMT5 TIM barrel domain; pfam17285"
                     /db_xref="CDD:435840"
     Region          295..463
                     /region_name="PRMT5"
                     /note="PRMT5 arginine-N-methyltransferase; pfam05185"
                     /db_xref="CDD:428356"
     Region          466..634
                     /region_name="PRMT5_C"
                     /note="PRMT5 oligomerization domain; pfam17286"
                     /db_xref="CDD:435841"
     CDS             1..636
                     /gene="csul"
                     /coded_by="XM_077647505.1:64..1974"
                     /db_xref="GeneID:144114053"
ORIGIN      
        1 maaqqkvscg vdlqfsanlr qglifagnag fkfvavdish prarrefieh dvktnprpfa
       61 rsdlvlpsqd wnslvtlkis kwidvdspve sfrkaseevl nqemcyaahl gvpailihvr
      121 srrcvnlsrm lhnfifasht yqawilipme pasqqsrqhl cslsgegnqc dnddnnnvdd
      181 fsrdedpwew wnmfrnvagp ekrlglalsl panipdesam rrwmgepvkc ivistsvflt
      241 nkkgfpvlsk ahqavlhqff klncqllldg rcrhkhmrfy yqyldhvfka rpqndplsef
      301 akgfedylqt plqplmdnle avtyevfekd pvkytqyqka ihlalvdkge qvgkdteiii
      361 mvvgagrgpl vraaltaael agqkvrlyai eknpnavitl lsqqtdswkd rvtvvscdmr
      421 dfdpsekadi lvsellgsfg dnelspecld gaqrflkddg isipckyssy lsplqsqkly
      481 ievaalrekd khplssyevp yvvrlhnvte laehqrlftf vhpnkdkthd nsrykslrfk
      541 vrdnsilhgf agyfdtvlyk dvtlsicpdt hspgmfswfp mffplkeplr lnkgstlevh
      601 fwrcvtqrkv wyewlvtepe igtihnpcgr syiigl
//
LOCUS       XP_077503632             538 aa            linear   INV 25-NOV-2025
DEFINITION  protein arginine N-methyltransferase 5 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503632
VERSION     XP_077503632.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647506.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..538
                     /product="protein arginine N-methyltransferase 5 isoform
                     X3"
                     /calculated_mol_wt=61018
     Region          30..293
                     /region_name="PRMT5_TIM"
                     /note="PRMT5 TIM barrel domain; pfam17285"
                     /db_xref="CDD:435840"
     Region          299..467
                     /region_name="PRMT5"
                     /note="PRMT5 arginine-N-methyltransferase; pfam05185"
                     /db_xref="CDD:428356"
     CDS             1..538
                     /gene="csul"
                     /coded_by="XM_077647506.1:64..1680"
                     /db_xref="GeneID:144114053"
ORIGIN      
        1 maaqqkvscg vdlqfsanlr qglifagnag fkfvavdish prarrefieh dvktnprpfa
       61 rsdlvlpsqd wnslvtlkis kwidvdspve sfrkaseevl nqemcyaahl gvpailihvr
      121 srrcvnlsrm lhnfifasht yqlmfqawil ipmepasqqs rqhlcslsge gnqcdnddnn
      181 nvddfsrded pwewwnmfrn vagpekrlgl alslpanipd esamrrwmge pvkcivists
      241 vfltnkkgfp vlskahqavl hqffklncql lldgrcrhkh mrfyyqyldh vfkarpqndp
      301 lsefakgfed ylqtplqplm dnleavtyev fekdpvkytq yqkaihlalv dkgeqvgkdt
      361 eiiimvvgag rgplvraalt aaelagqkvr lyaieknpna vitllsqqtd swkdrvtvvs
      421 cdmrdfdpse kadilvsell gsfgdnelsp ecldgaqrfl kddgisipck yssylsplqs
      481 qklyievaal rekdkhplav ccqrrcsshh tcsrifcrpl gwklgaqrps cislrrrs
//
LOCUS       XP_077503634             473 aa            linear   INV 25-NOV-2025
DEFINITION  Bardet-Biedl syndrome 4 [Amblyomma americanum].
ACCESSION   XP_077503634
VERSION     XP_077503634.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647508.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..473
                     /product="Bardet-Biedl syndrome 4"
                     /calculated_mol_wt=52899
     Region          <56..215
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Region          86..113
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          118..146
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(153,156..157,160..161,163,186,189..190,193..194,
                     196..197,220,223..224,227..228,231)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          153..180
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          157..413
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Region          185..214
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          219..247
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          253..281
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          287..315
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(288,291..292,295..296,298,322,325..326,329..330,
                     332..333,356,359..360,363..364,367)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          320..350
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          355..383
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          388..413
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     CDS             1..473
                     /gene="BBS4"
                     /coded_by="XM_077647508.1:1..1422"
                     /db_xref="GeneID:144114054"
ORIGIN      
        1 mansekqpvp gtsgtsvakg dgskstprds eqelssvrsr akkapehqsi drknwlihmh
       61 fvrrefdick alikemlaet kgyceyalyi qalilrhegn iseslqvfqs callngdpsi
      121 lkqvarslfl lgrhkaaadi yeecikmkgk cwdilhylgl cytklkdyeq arqrfsealq
      181 qssrdetfia lgelhiandd mksavnvfrk avennpdsae lntklgllym qcnmypkafe
      241 rlgaalasdp syapavlaag simqtygdyd valtkyrava svpdespalw nnvgmcffgk
      301 kkyvaaiscl kranylspmd wqilhnlglv hltmeqyasa yhffnaavhf nprngqlfml
      361 laialrnlqd tdnakrayer amaldeeplv clnyavlmhd lgdhkaaarl laqfeerclk
      421 qqqnsgveid aavtqmannl ssslqststd sksydevpds pdrndessqe rse
//
LOCUS       XP_077503635             164 aa            linear   INV 25-NOV-2025
DEFINITION  U-scoloptoxin(01)-Er1a-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503635
VERSION     XP_077503635.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647509.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..164
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..164
                     /product="U-scoloptoxin(01)-Er1a-like isoform X1"
                     /calculated_mol_wt=18189
     Region          51..107
                     /region_name="CBM_14"
                     /note="Chitin binding Peritrophin-A domain; pfam01607"
                     /db_xref="CDD:426342"
     CDS             1..164
                     /gene="LOC144114055"
                     /coded_by="XM_077647509.1:772..1266"
                     /db_xref="GeneID:144114055"
ORIGIN      
        1 mmklyillal laateaakvh artrraayel pdgvefvvgg vqttfqcerg gyfadvsnnc
       61 qlfhicneiy kedgsvelqq ytffcgnqtv fnqlsltcah peesvpcsna pdffyindni
      121 grvgtqahte ddlqraaplv pgfqqqqqfa asnkhetrll pppk
//
LOCUS       XP_077503636             164 aa            linear   INV 25-NOV-2025
DEFINITION  U-scoloptoxin(01)-Er1a-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503636
VERSION     XP_077503636.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647510.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..164
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..164
                     /product="U-scoloptoxin(01)-Er1a-like isoform X1"
                     /calculated_mol_wt=18189
     Region          51..107
                     /region_name="CBM_14"
                     /note="Chitin binding Peritrophin-A domain; pfam01607"
                     /db_xref="CDD:426342"
     CDS             1..164
                     /gene="LOC144114055"
                     /coded_by="XM_077647510.1:792..1286"
                     /db_xref="GeneID:144114055"
ORIGIN      
        1 mmklyillal laateaakvh artrraayel pdgvefvvgg vqttfqcerg gyfadvsnnc
       61 qlfhicneiy kedgsvelqq ytffcgnqtv fnqlsltcah peesvpcsna pdffyindni
      121 grvgtqahte ddlqraaplv pgfqqqqqfa asnkhetrll pppk
//
LOCUS       XP_077503637             164 aa            linear   INV 25-NOV-2025
DEFINITION  U-scoloptoxin(01)-Er1a-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503637
VERSION     XP_077503637.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647511.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..164
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..164
                     /product="U-scoloptoxin(01)-Er1a-like isoform X1"
                     /calculated_mol_wt=18189
     Region          51..107
                     /region_name="CBM_14"
                     /note="Chitin binding Peritrophin-A domain; pfam01607"
                     /db_xref="CDD:426342"
     CDS             1..164
                     /gene="LOC144114055"
                     /coded_by="XM_077647511.1:312..806"
                     /db_xref="GeneID:144114055"
ORIGIN      
        1 mmklyillal laateaakvh artrraayel pdgvefvvgg vqttfqcerg gyfadvsnnc
       61 qlfhicneiy kedgsvelqq ytffcgnqtv fnqlsltcah peesvpcsna pdffyindni
      121 grvgtqahte ddlqraaplv pgfqqqqqfa asnkhetrll pppk
//
LOCUS       XP_077503638             182 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114055 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503638
VERSION     XP_077503638.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647512.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..182
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..182
                     /product="uncharacterized protein LOC144114055 isoform X2"
                     /calculated_mol_wt=19967
     Region          69..125
                     /region_name="CBM_14"
                     /note="Chitin binding Peritrophin-A domain; pfam01607"
                     /db_xref="CDD:426342"
     CDS             1..182
                     /gene="LOC144114055"
                     /coded_by="XM_077647512.1:226..774"
                     /db_xref="GeneID:144114055"
ORIGIN      
        1 msgsqaeplh paisrgsamm klyillalla ateaakvhar trraayelpd gvefvvggvq
       61 ttfqcerggy fadvsnncql fhicneiyke dgsvelqqyt ffcgnqtvfn qlsltcahpe
      121 esvpcsnapd ffyindnigr vgtqahtedd lqraaplvpg fqqqqqfaas nkhetrllpp
      181 pk
//
LOCUS       XP_077503639             159 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114056 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503639
VERSION     XP_077503639.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647513.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..159
                     /product="uncharacterized protein LOC144114056 isoform X1"
                     /calculated_mol_wt=17493
     Region          17..151
                     /region_name="MARVEL"
                     /note="Membrane-associating domain; pfam01284"
                     /db_xref="CDD:366555"
     CDS             1..159
                     /gene="LOC144114056"
                     /coded_by="XM_077647513.1:187..666"
                     /db_xref="GeneID:144114056"
ORIGIN      
        1 mshtrtvtri svknedccsi pgllrvsetv fgticlglml hytkhsnvdv fgmpghelyf
       61 iltafgcllc tflllvsscw sftsssllsr tsfeilfhlv acalylaagi tlltktldyd
      121 rrfrgegyda riaaavfgim ngalyllgai lsfrifrqg
//
LOCUS       XP_077503640             167 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114056 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503640
VERSION     XP_077503640.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647514.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..167
                     /product="uncharacterized protein LOC144114056 isoform X2"
                     /calculated_mol_wt=18198
     Region          24..159
                     /region_name="MARVEL"
                     /note="Membrane-associating domain; pfam01284"
                     /db_xref="CDD:366555"
     CDS             1..167
                     /gene="LOC144114056"
                     /coded_by="XM_077647514.1:455..958"
                     /db_xref="GeneID:144114056"
ORIGIN      
        1 mshtvtvtrm ttttgsplli ntgyacniqg alkiletvfg ticlglmlhy tkhsnvdvfg
       61 mpghelyfil tafgcllctf lllvsscwsf tsssllsrts feilfhlvac alylaagitl
      121 ltktldydrr frgegydari aaavfgimng alyllgails frifrqg
//
LOCUS       XP_077503641            1403 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114058 [Amblyomma americanum].
ACCESSION   XP_077503641
VERSION     XP_077503641.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647515.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1403
                     /product="uncharacterized protein LOC144114058"
                     /calculated_mol_wt=157809
     Region          432..992
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1403
                     /gene="LOC144114058"
                     /coded_by="XM_077647515.1:1..4212"
                     /db_xref="GeneID:144114058"
ORIGIN      
        1 mgdsldflav wkeqtrgidd yvsllhrfft tidiplrarf hissvaqqpg thteeryyqa
       61 vckytfpdtg sravptsalq avsarvtqqw cllgrmmpfr ghlqwliper ehgfwcrwmp
      121 headiqlasl sfgtfaglcl faqshvfysk pgapgytlsc afkhderllq vmlklvhkns
      181 cshtcssykl qipyhsifrv vlneaethhd algiylqlvt apllyrsayd vneweastgd
      241 gvwydrvfql gcpcwwvmrs ydiggssvvk ltfrnklkar qaisrlsarc kpettfqysp
      301 mltkkvgakl reikmrchsk ikpnlcfptc yafsavlqqg hdgfaqmall acetleefee
      361 llirllhisg palelalyai rsaieeheiv diisclpklf akfctpaaal skapagtclv
      421 rrcivtpskv illppqihcq nrilrkfdpe fslrvsfrdd nmqnlsyslm sshcrhmvie
      481 rvvartlrdg lvagnrrfrl latscsqlrd hgawlyatda kgyssemirh wmgdfsgias
      541 tpkkmarmgq cfssteesvh vpllsnsvre vpdilggkhp vtaehyifsd gigmispgll
      601 kevtdkiglt vspsaiqiry agykgmlcvn pllsgrqivl rksmrkfncv nsecieviki
      661 saprpvflnr qlitlleqlg vpsrvffclq qrmvtlftea lvsdsvalqv letyvgpalp
      721 fgqllrhgfs ltrdpfvrsi lfavyrtsme sllsktriav prnlgrnmfg vldetgtlky
      781 gqvfvqftsl gsstrqqngp pvfilsgtvl vtkcpclhpg dvrkftavnv palhhikdcv
      841 vfpangfrph sdemagsdld gdeyiviwdr nlffpghnqe pmiygqkvqq qrselslvdg
      901 makfisdfik ndnvgvmsna hlamadklkd gvfskqclsi aakistcldf aktgvaavle
      961 kherpreypd fmakgchknt yrsnrvlghl yrfqrflesv vstsfnsqlv dgrsniklle
     1021 fhgwmsyrsv veelraayes dmdrilkqyg ikteaevvsg findtssfnk snyeksnvev
     1081 lvtkqyraia dstrkrffkk veeacylesa fsteeqttil lrmasacfmv tysnaqktcs
     1141 sfpwifseli llvmasasrr empyrqgnll iacldsqlpp assqlsaksi alevvlkwav
     1201 kedlllqdag vrcaricrhc letlfinsar aedaiangeg apspgcyvts ffryvgstsv
     1261 vfpkcdscsw ssqthtltma alrtysllai fcdlchlglp cdarlhepvq eiqegndvri
     1321 kvtspgfrdm llhgqdevvs llavwsgvhe vrirrgadrg ggrdivvssv grdwqrwfle
     1381 elllqpwlat avekrsleqf irg
//
LOCUS       XP_077503642            1403 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114059 [Amblyomma americanum].
ACCESSION   XP_077503642
VERSION     XP_077503642.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647516.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1403
                     /product="uncharacterized protein LOC144114059"
                     /calculated_mol_wt=157770
     Region          432..992
                     /region_name="RdRP"
                     /note="RNA dependent RNA polymerase; pfam05183"
                     /db_xref="CDD:461574"
     CDS             1..1403
                     /gene="LOC144114059"
                     /coded_by="XM_077647516.1:1..4212"
                     /db_xref="GeneID:144114059"
ORIGIN      
        1 mgdsldflav wkeqtrgidd yvsllhrfft tidiplsarf hissvaqqpg thteeryyqa
       61 vckytfpdtg sravptsalq avsarvtqqw cllgrmmpfr ghlqwliper ehgfwcrwmp
      121 headiqlasl sfgtfaglcl faqshvfysk pgapgytlsc afkhderllq vmlklvhkns
      181 cshtcssykl qipyhsifrv vlneaethhd algiylqlvt apllyrsayd vneweastgd
      241 gvwydrvfql gcpcwwvmrs ydiggssvvk ltfrnklkar qaisrlsarc kpettfqysp
      301 mltkkvgakl reikmrchsk ikpnlcfptc yafsavlqqg hdgfaqmall acetleefee
      361 llirllhisg palelalyai rsaieeheiv diisclpklf akfctpaaal skapagtclv
      421 rrcivtpskv illppqihcq nrilrkfdpe fslrvsfrdd nmqnlsyslm sshcrhmvie
      481 rvvartlrdg lvagnrrfrl latscsqlrd hgawlyatda kgyssemirh wmgdfsgias
      541 tpkkmarmgq cfssteesvh vpllsnsvre vpdilggkhp vtaehyifsd gigmispgll
      601 kevtdkiglt vspsaiqiry agykgmlcvn pllsgrqivl rksmrkfncv nsecieviki
      661 saprpvflnr qlitlleqlg vpsrvffclq qrmvtlftea lvsdsvalqv letyvgpalp
      721 fgqllrhgfs ltrdpfvrsi lfavyrtsme sllsktriav prnlgrnmfg eldetgtlky
      781 gqvfvqftsl gsstrqqngp pvfilsgtvl vtkcpclhpg dvrkftavnv palhhikdcv
      841 vfpangfrph sdemagsdld gdeyiviwdr nlffpghnqe pmiygqkvqq qrselslvdg
      901 makfisdfik ndnvgvmsna hlamadklkd gvfskqclsi aakistcldf aktgvaavle
      961 kherpreypd fmakgchknt yrsnrvlghl yrfqrflesv vstsfnsqlv dgrsniklle
     1021 fhgwmsyrsv veelraayes dmdrilkqyg ikteaevvsg findtssfnk snyeksnvev
     1081 lvtkqyraia dstrkrffkk veeacylesa fsteeqttil lrmasacfmv tysnaqktcs
     1141 sfpwifseli llvmasasrr empyrqgnll iacldsqlpp assqlsaksi alevvlkwav
     1201 kedlllqdag vrcaricrhc letlfinsar aedaiangeg apspgcyvts ffryvgstsv
     1261 vfpkcdscsw ssqthtltma alrtysllai fcdlchlglp cdarlhepvq eiqegndvri
     1321 kvtspgfrdm llhgqdevvs llavwsgvhe vrirrgadrg ggrdivvssv grdwqrwfle
     1381 elllqpwlat avekrsleqf irg
//
LOCUS       XP_077503643             376 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-(1,3)-fucosyltransferase C-like [Amblyomma americanum].
ACCESSION   XP_077503643
VERSION     XP_077503643.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647517.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..376
                     /product="alpha-(1,3)-fucosyltransferase C-like"
                     /calculated_mol_wt=43947
     Region          57..169
                     /region_name="Glyco_tran_10_N"
                     /note="Fucosyltransferase, N-terminal; pfam17039"
                     /db_xref="CDD:465336"
     Region          189..364
                     /region_name="Glyco_transf_10"
                     /note="Glycosyltransferase family 10 (fucosyltransferase)
                     C-term; pfam00852"
                     /db_xref="CDD:459962"
     CDS             1..376
                     /gene="LOC144114062"
                     /coded_by="XM_077647517.1:311..1441"
                     /db_xref="GeneID:144114062"
ORIGIN      
        1 maavtksgfl vivslvlflc lfmvaqnrqp lwrvfdafrl newsyspfys agangtrrlp
       61 rilmwtsfyg rwhgtlnder tveevasgcp akcfitndrr rlassdaivf hvrnldmndl
      121 pmrrsesqkw vfwsmepppy svfggfnymr dmfnwtmsyr rdsdvyeaya klvprndtda
      181 pkkdhralwk skqkqavwmv shcetdskre afvqelkkhl evdvygscgd hvcpksrgsa
      241 cyddfertyf ymlafensic kdyvtekffs alkydmvpvv fgganysqig parsyvdala
      301 frspkklaeh lvvlsrnyta yssyfkwker qrvvawdadf celcsklhsa hfqetssysd
      361 mrawwehqgr crtwap
//
LOCUS       XP_077503644             376 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-(1,3)-fucosyltransferase C-like [Amblyomma americanum].
ACCESSION   XP_077503644
VERSION     XP_077503644.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647518.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..376
                     /product="alpha-(1,3)-fucosyltransferase C-like"
                     /calculated_mol_wt=43947
     Region          57..169
                     /region_name="Glyco_tran_10_N"
                     /note="Fucosyltransferase, N-terminal; pfam17039"
                     /db_xref="CDD:465336"
     Region          189..364
                     /region_name="Glyco_transf_10"
                     /note="Glycosyltransferase family 10 (fucosyltransferase)
                     C-term; pfam00852"
                     /db_xref="CDD:459962"
     CDS             1..376
                     /gene="LOC144114062"
                     /coded_by="XM_077647518.1:447..1577"
                     /db_xref="GeneID:144114062"
ORIGIN      
        1 maavtksgfl vivslvlflc lfmvaqnrqp lwrvfdafrl newsyspfys agangtrrlp
       61 rilmwtsfyg rwhgtlnder tveevasgcp akcfitndrr rlassdaivf hvrnldmndl
      121 pmrrsesqkw vfwsmepppy svfggfnymr dmfnwtmsyr rdsdvyeaya klvprndtda
      181 pkkdhralwk skqkqavwmv shcetdskre afvqelkkhl evdvygscgd hvcpksrgsa
      241 cyddfertyf ymlafensic kdyvtekffs alkydmvpvv fgganysqig parsyvdala
      301 frspkklaeh lvvlsrnyta yssyfkwker qrvvawdadf celcsklhsa hfqetssysd
      361 mrawwehqgr crtwap
//
LOCUS       XP_077503645             376 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-(1,3)-fucosyltransferase C-like [Amblyomma americanum].
ACCESSION   XP_077503645
VERSION     XP_077503645.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647519.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..376
                     /product="alpha-(1,3)-fucosyltransferase C-like"
                     /calculated_mol_wt=43947
     Region          57..169
                     /region_name="Glyco_tran_10_N"
                     /note="Fucosyltransferase, N-terminal; pfam17039"
                     /db_xref="CDD:465336"
     Region          189..364
                     /region_name="Glyco_transf_10"
                     /note="Glycosyltransferase family 10 (fucosyltransferase)
                     C-term; pfam00852"
                     /db_xref="CDD:459962"
     CDS             1..376
                     /gene="LOC144114062"
                     /coded_by="XM_077647519.1:173..1303"
                     /db_xref="GeneID:144114062"
ORIGIN      
        1 maavtksgfl vivslvlflc lfmvaqnrqp lwrvfdafrl newsyspfys agangtrrlp
       61 rilmwtsfyg rwhgtlnder tveevasgcp akcfitndrr rlassdaivf hvrnldmndl
      121 pmrrsesqkw vfwsmepppy svfggfnymr dmfnwtmsyr rdsdvyeaya klvprndtda
      181 pkkdhralwk skqkqavwmv shcetdskre afvqelkkhl evdvygscgd hvcpksrgsa
      241 cyddfertyf ymlafensic kdyvtekffs alkydmvpvv fgganysqig parsyvdala
      301 frspkklaeh lvvlsrnyta yssyfkwker qrvvawdadf celcsklhsa hfqetssysd
      361 mrawwehqgr crtwap
//
LOCUS       XP_077503646             376 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-(1,3)-fucosyltransferase C-like [Amblyomma americanum].
ACCESSION   XP_077503646
VERSION     XP_077503646.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647520.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..376
                     /product="alpha-(1,3)-fucosyltransferase C-like"
                     /calculated_mol_wt=43947
     Region          57..169
                     /region_name="Glyco_tran_10_N"
                     /note="Fucosyltransferase, N-terminal; pfam17039"
                     /db_xref="CDD:465336"
     Region          189..364
                     /region_name="Glyco_transf_10"
                     /note="Glycosyltransferase family 10 (fucosyltransferase)
                     C-term; pfam00852"
                     /db_xref="CDD:459962"
     CDS             1..376
                     /gene="LOC144114062"
                     /coded_by="XM_077647520.1:178..1308"
                     /db_xref="GeneID:144114062"
ORIGIN      
        1 maavtksgfl vivslvlflc lfmvaqnrqp lwrvfdafrl newsyspfys agangtrrlp
       61 rilmwtsfyg rwhgtlnder tveevasgcp akcfitndrr rlassdaivf hvrnldmndl
      121 pmrrsesqkw vfwsmepppy svfggfnymr dmfnwtmsyr rdsdvyeaya klvprndtda
      181 pkkdhralwk skqkqavwmv shcetdskre afvqelkkhl evdvygscgd hvcpksrgsa
      241 cyddfertyf ymlafensic kdyvtekffs alkydmvpvv fgganysqig parsyvdala
      301 frspkklaeh lvvlsrnyta yssyfkwker qrvvawdadf celcsklhsa hfqetssysd
      361 mrawwehqgr crtwap
//
LOCUS       XP_077503647             324 aa            linear   INV 25-NOV-2025
DEFINITION  putative aminopeptidase W07G4.4 [Amblyomma americanum].
ACCESSION   XP_077503647
VERSION     XP_077503647.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647521.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..324
                     /product="putative aminopeptidase W07G4.4"
                     /calculated_mol_wt=34641
     Region          <1..319
                     /region_name="Peptidase_M17"
                     /note="Cytosol aminopeptidase family, N-terminal and
                     catalytic domains. Family M17 contains zinc- and
                     manganese-dependent exopeptidases (EC 3.4.11.1), including
                     leucine aminopeptidase. They catalyze removal of amino
                     acids from the N-terminus of a protein and...; cl29532"
                     /db_xref="CDD:475224"
     Site            order(43,45,78,84,89,92,95..96,129,131..134,136,138..142,
                     148,150,154,194..198,201,231..232,234,239,241..242,249,
                     251,265..266,268,307)
                     /site_type="other"
                     /note="interface (dimer of trimers) [polypeptide binding]"
                     /db_xref="CDD:238247"
     Site            order(74,79,86,97,157,159,161,189)
                     /site_type="active"
                     /note="Substrate-binding/catalytic site [active]"
                     /db_xref="CDD:238247"
     Site            order(74,79,97,157,159)
                     /site_type="other"
                     /note="Zn-binding sites [ion binding]"
                     /db_xref="CDD:238247"
     CDS             1..324
                     /gene="LOC144114063"
                     /coded_by="XM_077647521.1:430..1404"
                     /db_xref="GeneID:144114063"
ORIGIN      
        1 maaprvadyv qrvfrdspvk veviadeati esnypllaav nraargvarh qcrmihlsye
       61 pegpvnktlf lvgkgvtydt ggadvkaggh magmhrdkcg aaavagffqv lcklrpknir
      121 vygamamvrn sigsecyvsd eiitsragvr vrigntdaeg rmamadalce ykdkalkevn
      181 priftiatlt ghacmavgeg ysiiidngpa rasavskvvq aagdrigdpf evstlrredy
      241 dfvrgpseye dvlqcnneps srtprghqmp aaflvrvsgl dkhgvdseqp lcyshidiaa
      301 ssgpypgvps gapiaaltqa flpl
//
LOCUS       XP_077503648             214 aa            linear   INV 25-NOV-2025
DEFINITION  ribosomal protein L13 [Amblyomma americanum].
ACCESSION   XP_077503648
VERSION     XP_077503648.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647522.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by mRNA and EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..214
                     /product="ribosomal protein L13"
                     /calculated_mol_wt=24807
     Region          8..187
                     /region_name="Ribosomal_L13e"
                     /note="Ribosomal protein L13e; pfam01294"
                     /db_xref="CDD:460150"
     CDS             1..214
                     /gene="RpL13"
                     /coded_by="XM_077647522.1:137..781"
                     /db_xref="GeneID:144114064"
ORIGIN      
        1 mapkrnnmvp nghfhkdwqr yvktwfnqpm rkkrrhanrv kkaraiaprp lkgslrpvir
       61 cpgfkyntkq rlgkgftlee lkaagidkra artigisvdy rrrnksvesl qqnvqrlkey
      121 rsklilfplk gsksektevs tdeqklalqy kgvimplkks thyekprvpt eeekkycayv
      181 hlrqakakar lwgkrakkar eaaesmeaqa pkka
//
LOCUS       XP_077503649             214 aa            linear   INV 25-NOV-2025
DEFINITION  ribosomal protein L13 [Amblyomma americanum].
ACCESSION   XP_077503649
VERSION     XP_077503649.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647523.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..214
                     /product="ribosomal protein L13"
                     /calculated_mol_wt=24807
     Region          8..187
                     /region_name="Ribosomal_L13e"
                     /note="Ribosomal protein L13e; pfam01294"
                     /db_xref="CDD:460150"
     CDS             1..214
                     /gene="RpL13"
                     /coded_by="XM_077647523.1:143..787"
                     /db_xref="GeneID:144114064"
ORIGIN      
        1 mapkrnnmvp nghfhkdwqr yvktwfnqpm rkkrrhanrv kkaraiaprp lkgslrpvir
       61 cpgfkyntkq rlgkgftlee lkaagidkra artigisvdy rrrnksvesl qqnvqrlkey
      121 rsklilfplk gsksektevs tdeqklalqy kgvimplkks thyekprvpt eeekkycayv
      181 hlrqakakar lwgkrakkar eaaesmeaqa pkka
//
LOCUS       XP_077503650             886 aa            linear   INV 25-NOV-2025
DEFINITION  leucyl-tRNA synthetase, mitochondrial isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503650
VERSION     XP_077503650.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647524.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..886
                     /product="leucyl-tRNA synthetase, mitochondrial isoform
                     X1"
                     /calculated_mol_wt=101431
     Region          38..838
                     /region_name="LeuS"
                     /note="Leucyl-tRNA synthetase [Translation, ribosomal
                     structure and biogenesis]; COG0495"
                     /db_xref="CDD:440261"
     CDS             1..886
                     /gene="LeuRS-m"
                     /coded_by="XM_077647524.1:82..2742"
                     /db_xref="GeneID:144114068"
ORIGIN      
        1 mlmiirvlak kqqqrdlrni fsltsvwdpk isastrlkve efwkdkinqv ppkhvdpakk
       61 myvlsmfpyp sgqlhlghvr vyaisdtyar fyamhgkqvi hpmgwdsfgl paenaavern
      121 enprawtekn iesmkkqlkd lnfnfdwsre lstcdpgyyk wtqylflkly ekglvyqkna
      181 vvhwdpvdkt vladeqvdae grswrsgavv ekknlrqwfi rttylskdll sglcdsernd
      241 wddvgpvqrg wigectgfsf ewtvidscgt phsdllrvwt eqpeflgavt fvalspdhsl
      301 alsiangsds aagkssyyal npisgkkvpl lvsskfefpr atschlgtps lskddlefav
      361 ehgievikvv ddhnpshlin sgtltglsak aarlqvcqqa qlegrggypc sshlkdwlis
      421 rqrywgtpip ivhcrtcgvv pvpceelpve lplldkitke gvsplcgahd wlstscprcs
      481 gpatretdtm dtfvdsswyy lrfldpnnse kivdptvshd lmpvdlyigg lehavmhlyy
      541 arfvnhfchg lglsatkepf lrmlvhgmvv netfkvcatg qylprdtviq kgsklfhkdt
      601 gepvvvdwek mskskhngvr pedvvsehgi dttrlfilca ysptsrykwp spafrgivsw
      661 qerlwltvhd frnsagrsle eaqstpefad reahyyhgrn hhvknvtfmy nvtrqlnlvi
      721 kdlqiftkll ryknrgnvvf greferalaa lllmlaplap hfaselwagf tdaarfrdvd
      781 yqldrplmeq lwpeidpdfn ldllikednv dlmriqlprh dferltkesa yqmaiesesl
      841 rhrlqkgpkf rvvfdhypsy kaelhfisrg knaeelqrvh sqrhie
//
LOCUS       XP_077503651             808 aa            linear   INV 25-NOV-2025
DEFINITION  leucyl-tRNA synthetase, mitochondrial isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503651
VERSION     XP_077503651.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647525.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..808
                     /product="leucyl-tRNA synthetase, mitochondrial isoform
                     X2"
                     /calculated_mol_wt=92272
     Region          51..760
                     /region_name="LeuS"
                     /note="Leucyl-tRNA synthetase [Translation, ribosomal
                     structure and biogenesis]; COG0495"
                     /db_xref="CDD:440261"
     CDS             1..808
                     /gene="LeuRS-m"
                     /coded_by="XM_077647525.1:131..2557"
                     /db_xref="GeneID:144114068"
ORIGIN      
        1 msawdtyhst hlqssspafd snvtpppqcq rqspsplplr lretrrrgqc rniesmkkql
       61 kdlnfnfdws relstcdpgy ykwtqylflk lyekglvyqk navvhwdpvd ktvladeqvd
      121 aegrswrsga vvekknlrqw firttylskd llsglcdser ndwddvgpvq rgwigectgf
      181 sfewtvidsc gtphsdllrv wteqpeflga vtfvalspdh slalsiangs dsaagkssyy
      241 alnpisgkkv pllvsskfef pratschlgt pslskddlef avehgievik vvddhnpshl
      301 insgtltgls akaarlqvcq qaqlegrggy pcsshlkdwl isrqrywgtp ipivhcrtcg
      361 vvpvpceelp velplldkit kegvsplcga hdwlstscpr csgpatretd tmdtfvdssw
      421 yylrfldpnn sekivdptvs hdlmpvdlyi gglehavmhl yyarfvnhfc hglglsatke
      481 pflrmlvhgm vvnetfkvca tgqylprdtv iqkgsklfhk dtgepvvvdw ekmskskhng
      541 vrpedvvseh gidttrlfil caysptsryk wpspafrgiv swqerlwltv hdfrnsagrs
      601 leeaqstpef adreahyyhg rnhhvknvtf mynvtrqlnl vikdlqiftk llryknrgnv
      661 vfgreferal aalllmlapl aphfaselwa gftdaarfrd vdyqldrplm eqlwpeidpd
      721 fnldlliked nvdlmriqlp rhdferltke sayqmaiese slrhrlqkgp kfrvvfdhyp
      781 sykaelhfis rgknaeelqr vhsqrhie
//
LOCUS       XP_077503653             155 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114069 [Amblyomma americanum].
ACCESSION   XP_077503653
VERSION     XP_077503653.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647527.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..155
                     /product="uncharacterized protein LOC144114069"
                     /calculated_mol_wt=16448
     CDS             1..155
                     /gene="LOC144114069"
                     /coded_by="XM_077647527.1:203..670"
                     /db_xref="GeneID:144114069"
ORIGIN      
        1 mpvtvtqqtt tvsssggner lalcafnfgy lktpdgwiki aqvvvglivm slltktyqas
       61 wvvflmmtgf afavvsfiil lsamgsysgs lligtlfyln yhivgfffyl sggltcvvna
      121 sqyvgldkvi aagvlglfas alyavdvalt vrarv
//
LOCUS       XP_077503654             288 aa            linear   INV 25-NOV-2025
DEFINITION  signal sequence receptor subunit 1 l(1)G0320 [Amblyomma
            americanum].
ACCESSION   XP_077503654
VERSION     XP_077503654.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647528.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..288
                     /product="signal sequence receptor subunit 1 l(1)G0320"
                     /calculated_mol_wt=31958
     Region          10..285
                     /region_name="TRAP_alpha"
                     /note="Translocon-associated protein (TRAP), alpha
                     subunit; pfam03896"
                     /db_xref="CDD:461085"
     CDS             1..288
                     /gene="l(1)G0320"
                     /coded_by="XM_077647528.1:141..1007"
                     /db_xref="GeneID:144114070"
ORIGIN      
        1 mlfryiival livplatifh qsgppsalaq eeddvvveda apsgseqqea avseqdddde
       61 gddstsggpl kpsphgdtyl lftkpqnfqs klpagkevhf lvgfsnkger dfvldtmdas
      121 frypmdftfy iqnfttigyn kvvkpkqqat lsysfftses fsarpfglav nlryrdadgn
      181 afqdavfnet ievveidegl dgetfflymf latclvlllv aaqqflgtls kkkpaskqkv
      241 emgttnasdv dydwlpketr dlnrspkrsp rqsprsrrlk rgtgsgee
//
LOCUS       XP_077503655            1282 aa            linear   INV 25-NOV-2025
DEFINITION  5-oxoprolinase [Amblyomma americanum].
ACCESSION   XP_077503655
VERSION     XP_077503655.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647529.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1282
                     /product="5-oxoprolinase"
                     /calculated_mol_wt=139005
     Region          10..1265
                     /region_name="PLN02666"
                     /note="5-oxoprolinase"
                     /db_xref="CDD:215358"
     CDS             1..1282
                     /gene="LOC144114071"
                     /coded_by="XM_077647529.1:216..4064"
                     /db_xref="GeneID:144114071"
ORIGIN      
        1 msavpdsslk fqfaidrggt ftdiyarcpg gkikvmklls edpnnypdap regirrileq
       61 etgkaypene ridasmiewi rmgttvatna llerkgepta fvvtagfrdl lyignqarph
      121 lfdltcakpe vlydyvvead ervalcrddc cldldtpefl gttdekvtii kpldmqklkv
      181 dlqkvldqgi kslavalihs ytfkehellv gqcatelgfe hvslssdvmp mvrivprgft
      241 acvdayltpc ikkyvksfac gfknnlkdvp vlfmqsdggl tpmetfsgsr silsgpaggv
      301 vgyaktsyne edkvpvigfd mggtstdvsr ydghfdhtfe tttagvtiqa pqldistvaa
      361 gggsmlffrs glfvvgpesa gahpgpacyr kggpltvtda nvclgrllpd hfpkifghse
      421 repidkeasl safrrlthev nlflksqpgg ankhmtleev amgfvnvane smcrpiralt
      481 qgkgfdvsnh llacfggaga qhacaiartl gmrkvlihry agilsaygma ladvveeaqe
      541 pcamtytagn iplidarika lskkctealc qqgfdkqnil vepflhmryd rtdcalmcpc
      601 eviksgssvp sasadgqtts afgdfessfe srykrefgfv lpgrsiivdd irvravgrnt
      661 ipeseclapg seqqlqpvei vlcyfedcfh ptpvyllktl lagnsingpa iiiddnctiv
      721 veplcraqvl khgdisievg kskrkrigle ldavrlslfs hrfmsiaeqm gkilqrtsvs
      781 tnikerldfs calfgpdggl vcnaphipvh lgamqeavqy qmmmlgdnla pgdvllsnhp
      841 haggshlpdl tiitpvfykd eprpvffvan rghhadiggi apgsmpahsh tileegatfv
      901 sfkvvqagvf qqealikaln apaflpncsg trnlrdnlad iqaqiaanqk giilvkelid
      961 hygleavqay mgyvqdnaev airdllrvva hsaisklgka elhawdrmdd gsricltvkl
     1021 nekegsavfd fsgtgrevyg nlnapravtl sailyclrcm vghdlplnqg clapvqvllp
     1081 rnsllnpsad aavvggnvlt sqrvvdvifh afntcaasqg cmnnitfgde tlgyyetigg
     1141 gagagphwhg rsgihthmtn tritdpeile rrypvilqry hlnigtggqg kfsggdgvvr
     1201 ellfrkhlql svlterrvfs pyglqggypg scginllffk sgrvvnlgsk asvrispgdl
     1261 lhvetpgggg ygppfsppek kv
//
LOCUS       XP_077503656             429 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114072 [Amblyomma americanum].
ACCESSION   XP_077503656
VERSION     XP_077503656.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647530.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..429
                     /product="uncharacterized protein LOC144114072"
                     /calculated_mol_wt=47521
     CDS             1..429
                     /gene="LOC144114072"
                     /coded_by="XM_077647530.1:1..1290"
                     /db_xref="GeneID:144114072"
ORIGIN      
        1 massdsnels rrgmhnktfq rhlkntlqpa atqaaaaama qvaqavakvy ddfcfghrgn
       61 iamcydgtwm trghsshigv gavvelftgy vldyvllsnf clgcetgpkl ntggyelwrs
      121 hhecqkntnc kagqmeveag kirferslqr hnmryttmlc dgdsrtfnvl qdakiygyie
      181 vvkvdcinhv hkrmgaalrn llqrhkgagk qslggrgrlt adlvdklaiy ygqalrsnvg
      241 nvdamsavma tyyhitstdd cpnhtlcptg eqswcrhnaa kakeepepkh kyslpsdvae
      301 allpvysrls eksllqrcir gktqnsnesf hsiiwtlihk erhsslfave aataeavlrf
      361 nagckhtata ilqecngnmp atvsrrakgk dlrrnarsak kraaslsfak aekkkhrdsm
      421 hpdyapgaf
//
LOCUS       XP_077503657             154 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114073 [Amblyomma americanum].
ACCESSION   XP_077503657
VERSION     XP_077503657.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647531.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..154
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..154
                     /product="uncharacterized protein LOC144114073"
                     /calculated_mol_wt=16791
     CDS             1..154
                     /gene="LOC144114073"
                     /coded_by="XM_077647531.1:137..601"
                     /db_xref="GeneID:144114073"
ORIGIN      
        1 mptpfysgde vggneqdwnn egfrarvqdt lrlltaleql pgtdpmlldv lrflilqelv
       61 mdlnsvlnqq aaegepampe padeapfsig saveeatrqm ydqlrnrqee eqpsdlqshl
      121 aavssaavph vnsdhsggqk tpptaepnpr epdc
//
LOCUS       XP_077503658            2069 aa            linear   INV 25-NOV-2025
DEFINITION  MAM and LDL-receptor class A domain-containing protein 2-like
            [Amblyomma americanum].
ACCESSION   XP_077503658
VERSION     XP_077503658.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647532.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2069
                     /product="MAM and LDL-receptor class A domain-containing
                     protein 2-like"
                     /calculated_mol_wt=224425
     Region          28..186
                     /region_name="MAM"
                     /note="MAM domain, meprin/A5/mu; pfam00629"
                     /db_xref="CDD:459878"
     Region          189..362
                     /region_name="MAM"
                     /note="Domain in meprin, A5, receptor protein tyrosine
                     phosphatase mu (and others); smart00137"
                     /db_xref="CDD:214533"
     Region          375..531
                     /region_name="MAM"
                     /note="Meprin, A5 protein, and protein tyrosine
                     phosphatase Mu (MAM) domain. MAM is an extracellular
                     domain which mediates protein-protein interactions and is
                     found in a diverse set of proteins, many of which are
                     known to function in cell adhesion. Members...; cd06263"
                     /db_xref="CDD:99706"
     Region          540..704
                     /region_name="MAM"
                     /note="Meprin, A5 protein, and protein tyrosine
                     phosphatase Mu (MAM) domain. MAM is an extracellular
                     domain which mediates protein-protein interactions and is
                     found in a diverse set of proteins, many of which are
                     known to function in cell adhesion. Members...; cd06263"
                     /db_xref="CDD:99706"
     Region          723..889
                     /region_name="MAM"
                     /note="MAM domain, meprin/A5/mu; pfam00629"
                     /db_xref="CDD:459878"
     Region          900..1051
                     /region_name="MAM"
                     /note="MAM domain, meprin/A5/mu; pfam00629"
                     /db_xref="CDD:459878"
     Region          1058..1198
                     /region_name="MAM"
                     /note="Meprin, A5 protein, and protein tyrosine
                     phosphatase Mu (MAM) domain. MAM is an extracellular
                     domain which mediates protein-protein interactions and is
                     found in a diverse set of proteins, many of which are
                     known to function in cell adhesion. Members...; cd06263"
                     /db_xref="CDD:99706"
     Region          1238..1395
                     /region_name="MAM"
                     /note="MAM domain, meprin/A5/mu; pfam00629"
                     /db_xref="CDD:459878"
     Region          1400..1556
                     /region_name="MAM"
                     /note="MAM domain, meprin/A5/mu; pfam00629"
                     /db_xref="CDD:459878"
     Region          1561..1701
                     /region_name="MAM"
                     /note="Meprin, A5 protein, and protein tyrosine
                     phosphatase Mu (MAM) domain. MAM is an extracellular
                     domain which mediates protein-protein interactions and is
                     found in a diverse set of proteins, many of which are
                     known to function in cell adhesion. Members...; cd06263"
                     /db_xref="CDD:99706"
     Region          1683..>1841
                     /region_name="PHA03255"
                     /note="BDLF3; Provisional"
                     /db_xref="CDD:165513"
     Region          1857..1889
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(1862,1871,1882..1883)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1875,1878,1882,1888..1889)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1885..1889
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1891..1932
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1896,1911,1922..1923)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1915,1918,1922,1928..1929)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1925..1929
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     CDS             1..2069
                     /gene="LOC144114074"
                     /coded_by="XM_077647532.1:86..6295"
                     /db_xref="GeneID:144114074"
ORIGIN      
        1 mgrrcllwrt fgllcwlqaf agasdvecdf etgvcefsss ckggdcfvpv raklvdegpt
       61 idhtigtgdg wysrvqfsks awhesvarle lsttgpfcfl awvhasglkl prvemasrhe
      121 nadwhvdead kervffraqs shselwqkvq yneerqgamk leirawhftn gsvgvddlsl
      181 asgpcpedpa dgscsfdrsv cgyenavsgs gnkwklvppg rsasrnrava ldhttdtmrg
      241 gyvhlfvpan ttssrsltsp llsepkgqhs rcvrffyyap ppktptglel yldsnpgtsl
      301 psdgfagmer lwsvdssslt sglwmpvevg ftvhtkhevt frayvdvpqn sswycalddi
      361 evyacadqkg eqikcnfdng hmcnwtallq psgpagvwkl sdlrwgdphf pdrdhtqgta
      421 dggfvyaenr dggsvlkavl tsptldagwv gaacltfwhf avfdepascn ltvsplsgdp
      481 wwssthkler awkqetvraw lradqrqftl easltdalia vddveltfga csseqrglsc
      541 dfergpctwi npmakrrtwe wllrggplkt dlpqppndht lssadgsfml vsgremllrg
      601 saelqsevvn lkaagvqcme fwytahgsrd aeltfkvivq qttpgaakks eeiwshkggn
      661 vtawqlgrvk iprrhrvvfe aavlqsrdgy ialddisiyi ndhcstipae atsgkaayvl
      721 lecswntpks clwsrpeeia gtwklgsrfa pksalapaas pdgkpnnfiy lsceklhqgs
      781 svvsaslnsp iveqqhtavc vrfafhmfgp arrqlvfalg trqdfasvkt kttalyqahg
      841 gtvadrwhhl artvrfasaa ttieffassd kcergdials dihvtpgpct adaavqglsd
      901 fehdmgfwhn ngwehvltgm sgdmasatlr sgpadsagfl qltstgspkt aslrsfewha
      961 sdqprtielw yygnvtgnws lgvhladkkg splvrvwtlp sapemktwsl araeipvqse
     1021 dftvviegtv wprpgtnalv avdnvilglt pplhvancdf qldlcgyigs fdsdfrwlvg
     1081 sgrvfrsqpg aptyeaissg aghagsfayv dptvpmtpss kltatirsaf fnssgnenmt
     1141 vhyfrngtaf tsfqvhqlvg pgggqktsri lladlasgdq wqdvvlplrp agecqleftl
     1201 tgtpkekkgl aaiasisvis agkgqlytna lrfadlscdf dqgsyclwks eaaadgqplw
     1261 alndpatgrp pfplydrstw nqsghfifsr nngsvaslsr iksiplsrgw tpsshcfsfw
     1321 yfmstdrnss lsvtltpsks hswsepgarm aawapavvrf netkvakkaq ivleasidsg
     1381 lvavddfavt lggcpraplc tfeggvecdh tadmnnvrdw tvqqgsqlgv pdhtlrnmlg
     1441 hylylntssv rqsrnsiari ylssrdatag acltfwwrsh gdqselsvyr faqaeglgda
     1501 lvslnteenl mwnglsvnit sdrpwqavfe alfpnyatte sgvllddiel adgacpdqns
     1561 ctfegptcva wqdandpyat gqwelqradl svfpsdhtmk tsqghylrfs aevpgklaal
     1621 taggasgdgp rcatfwylls delqgltlla gpayfnesth sqwqlaqfsl tryhrsiiav
     1681 sgtnpkafal iddllvyddd cawqgdysta atagtevttl kvttdsgttt tpstsrataa
     1741 pgiesttvvl tspvtstaeh eattteavit dsvattvpst lsgtgttqse ttaavskpsv
     1801 tdtaseatav asmtavtvtt aiasvtdeka sptepatssa tpetsasstt asssrgcgig
     1861 mfscrdgvhc vpalllcdgv rdcpngadei cgdhqfcpdg hyycqtppsc lgrsklcdgf
     1921 hdcedgsdel lcfvcpgffc rnegncgfhp asgapncsck pgfdgnrcqr ppvlqptsar
     1981 vagatgtway vapvlvlvil astvgiavyl rkkrrneide trqvvvenpi ygldlsqint
     2041 prqsrrsaps wfsrtfrfsn atgisatec
//
LOCUS       XP_077503659            1495 aa            linear   INV 25-NOV-2025
DEFINITION  NACHT domain- and WD repeat-containing protein 1 isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077503659
VERSION     XP_077503659.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647533.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1495
                     /product="NACHT domain- and WD repeat-containing protein 1
                     isoform X1"
                     /calculated_mol_wt=165861
     Region          563..703
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            568..575
                     /site_type="other"
                     /note="Walker A motif"
                     /db_xref="CDD:99707"
     Site            order(569..576,659)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:99707"
     Site            655..660
                     /site_type="other"
                     /note="Walker B motif"
                     /db_xref="CDD:99707"
     Region          1148..1454
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          1170..1215
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1254..1294
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1299..1342
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1348..1383
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1389..1427
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1433..1475
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..1495
                     /gene="LOC144114075"
                     /coded_by="XM_077647533.1:81..4568"
                     /db_xref="GeneID:144114075"
ORIGIN      
        1 mkrtpqpslh tsrelcfstg asasggwwny fgsvdepqrf glagdpwkcn wqpltfeese
       61 drarvmgssc ssaqkkdker skkkkkgscs pegrrrssvr stdslpygke stpppsrsdp
      121 svtaitgsta pplapvdtsl tevqqnsgsl aspapqvtsp apgsetfsdd glpslqafpd
      181 dvqalllgrl gavpasllaa gsrlislyvc cgladmeaer thleqhvyps vrayaadkgy
      241 dlnivdlhwg iqveamdrii fthlclqtia rlrsqghfla lvllneklen qlphilpasl
      301 psdvltsvad avespeqrql lldwymedln svpkcyrlha ireripdiis tnpsakaaal
      361 elwqkessqm vealrsalta eqrakylvsv veeeleaavl ndsqkplccl wmerrfthrg
      421 pqrsnsvvgt liepaegsgp vdlsdskryn lvraqledrl pesqklvlrv kwsregptpt
      481 enaeyfdels ttlsrdlkam vdtvveedtt dettrpcrgv eqqlyvelvq qaqtclslse
      541 afigraemlt rlekylrskl rnpivlhgvk gsgkssllar vaqlcetwvp eapviarfvg
      601 tspdsdtqeq llrsicehcc alygmhaila sksiteqhea lgvlldkvsh qrpliilldg
      661 ldqmtkhsac gfdwlpselp phvkvvltvr dgskeledlk aklknqeqcf idlgpcgvde
      721 ckdifhksle rrsrtvsakq mesisaslqq spspllaall asavlhwhgt deptifhsed
      781 evasflfdql kkevdpkvts lvlgllsasr hglsdkeitd vvssyesvls gmphvhqkrp
      841 lkrcpfavwa lvhagldsfl rvrvvegyvl ntwsgdlyrt lcrehgpgve athvcilalf
      901 dyfqsvltae tssiilssas evdsgsqcvl pnvpkhtvfs nrrkhnelpf yclhlnhlar
      961 eklanvdall srlqvangpa gdgapvkygd sgdssealsq ivriqfllnp ewlqvkvccc
     1021 dpyflleeld mflnlnpgdt ecamlrelvq lssyalrydg rqfaahvtar lrnviateas
     1081 slkfprlknl yemaqrfpsl vpvsdylrep srkpvpavls eeergrtlgq lytirgdash
     1141 mvslnrsdll vwdvfgesiv rrltglsepr dlkmvdrfra lvlcnrelkv ysldegrllv
     1201 klkgvmnqkm ayfglhsqdy vvalsrnrmy vnmlnltsgd lettfkvged rflnsllvsg
     1261 ngkicvcgde tqkpfpllvw dlanrkllyd lriphhefit rlsaisgdgh yvvcvcrels
     1321 dsapnfiivy dlqsgtlfkk wkpernscsi aissqggcvv ngldncfvlv wdlatgarrf
     1381 tlkghnapvd qirldesgfk sltynsdgid rsvrvwdvmk geclavftpd lavtcceltl
     1441 dgkavvmgle gydrvfchll hdgegdqslp esrlasntyg nvsncgkvfd vsqqg
//
LOCUS       XP_077503660            1494 aa            linear   INV 25-NOV-2025
DEFINITION  NACHT domain- and WD repeat-containing protein 1 isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077503660
VERSION     XP_077503660.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647534.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1494
                     /product="NACHT domain- and WD repeat-containing protein 1
                     isoform X2"
                     /calculated_mol_wt=165774
     Region          562..702
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            567..574
                     /site_type="other"
                     /note="Walker A motif"
                     /db_xref="CDD:99707"
     Site            order(568..575,658)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:99707"
     Site            654..659
                     /site_type="other"
                     /note="Walker B motif"
                     /db_xref="CDD:99707"
     Region          1147..1453
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          1169..1214
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1253..1293
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1298..1341
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1347..1382
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1388..1426
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1432..1474
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..1494
                     /gene="LOC144114075"
                     /coded_by="XM_077647534.1:82..4566"
                     /db_xref="GeneID:144114075"
ORIGIN      
        1 mkrtpqpslh tsrelcfstg asasggwwny fgsvdepqrf glagdpwkcn wqpltfeese
       61 drarvmgssc ssaqkkdker skkkkkgcsp egrrrssvrs tdslpygkes tpppsrsdps
      121 vtaitgstap plapvdtslt evqqnsgsla spapqvtspa pgsetfsddg lpslqafpdd
      181 vqalllgrlg avpasllaag srlislyvcc gladmeaert hleqhvypsv rayaadkgyd
      241 lnivdlhwgi qveamdriif thlclqtiar lrsqghflal vllneklenq lphilpaslp
      301 sdvltsvada vespeqrqll ldwymedlns vpkcyrlhai reripdiist npsakaaale
      361 lwqkessqmv ealrsaltae qrakylvsvv eeeleaavln dsqkplcclw merrfthrgp
      421 qrsnsvvgtl iepaegsgpv dlsdskrynl vraqledrlp esqklvlrvk wsregptpte
      481 naeyfdelst tlsrdlkamv dtvveedttd ettrpcrgve qqlyvelvqq aqtclslsea
      541 figraemltr lekylrsklr npivlhgvkg sgkssllarv aqlcetwvpe apviarfvgt
      601 spdsdtqeql lrsicehcca lygmhailas ksiteqheal gvlldkvshq rpliilldgl
      661 dqmtkhsacg fdwlpselpp hvkvvltvrd gskeledlka klknqeqcfi dlgpcgvdec
      721 kdifhksler rsrtvsakqm esisaslqqs pspllaalla savlhwhgtd eptifhsede
      781 vasflfdqlk kevdpkvtsl vlgllsasrh glsdkeitdv vssyesvlsg mphvhqkrpl
      841 krcpfavwal vhagldsflr vrvvegyvln twsgdlyrtl crehgpgvea thvcilalfd
      901 yfqsvltaet ssiilssase vdsgsqcvlp nvpkhtvfsn rrkhnelpfy clhlnhlare
      961 klanvdalls rlqvangpag dgapvkygds gdssealsqi vriqfllnpe wlqvkvcccd
     1021 pyflleeldm flnlnpgdte camlrelvql ssyalrydgr qfaahvtarl rnviateass
     1081 lkfprlknly emaqrfpslv pvsdylreps rkpvpavlse eergrtlgql ytirgdashm
     1141 vslnrsdllv wdvfgesivr rltglseprd lkmvdrfral vlcnrelkvy sldegrllvk
     1201 lkgvmnqkma yfglhsqdyv valsrnrmyv nmlnltsgdl ettfkvgedr flnsllvsgn
     1261 gkicvcgdet qkpfpllvwd lanrkllydl riphhefitr lsaisgdghy vvcvcrelsd
     1321 sapnfiivyd lqsgtlfkkw kpernscsia issqggcvvn gldncfvlvw dlatgarrft
     1381 lkghnapvdq irldesgfks ltynsdgidr svrvwdvmkg eclavftpdl avtcceltld
     1441 gkavvmgleg ydrvfchllh dgegdqslpe srlasntygn vsncgkvfdv sqqg
//
LOCUS       XP_077503661            1478 aa            linear   INV 25-NOV-2025
DEFINITION  NACHT domain- and WD repeat-containing protein 1 isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077503661
VERSION     XP_077503661.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647535.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1478
                     /product="NACHT domain- and WD repeat-containing protein 1
                     isoform X3"
                     /calculated_mol_wt=163892
     Region          546..686
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            551..558
                     /site_type="other"
                     /note="Walker A motif"
                     /db_xref="CDD:99707"
     Site            order(552..559,642)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:99707"
     Site            638..643
                     /site_type="other"
                     /note="Walker B motif"
                     /db_xref="CDD:99707"
     Region          1131..1437
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          1153..1198
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1237..1277
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1282..1325
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1331..1366
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1372..1410
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1416..1458
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..1478
                     /gene="LOC144114075"
                     /coded_by="XM_077647535.1:121..4557"
                     /db_xref="GeneID:144114075"
ORIGIN      
        1 mtgasasggw wnyfgsvdep qrfglagdpw kcnwqpltfe esedrarvmg sscssaqkkd
       61 kerskkkkkg scspegrrrs svrstdslpy gkestpppsr sdpsvtaitg stapplapvd
      121 tsltevqqns gslaspapqv tspapgsetf sddglpslqa fpddvqalll grlgavpasl
      181 laagsrlisl yvccgladme aerthleqhv ypsvrayaad kgydlnivdl hwgiqveamd
      241 riifthlclq tiarlrsqgh flalvllnek lenqlphilp aslpsdvlts vadavespeq
      301 rqllldwyme dlnsvpkcyr lhaireripd iistnpsaka aalelwqkes sqmvealrsa
      361 ltaeqrakyl vsvveeelea avlndsqkpl cclwmerrft hrgpqrsnsv vgtliepaeg
      421 sgpvdlsdsk rynlvraqle drlpesqklv lrvkwsregp tptenaeyfd elsttlsrdl
      481 kamvdtvvee dttdettrpc rgveqqlyve lvqqaqtcls lseafigrae mltrlekylr
      541 sklrnpivlh gvkgsgkssl larvaqlcet wvpeapviar fvgtspdsdt qeqllrsice
      601 hccalygmha ilasksiteq healgvlldk vshqrpliil ldgldqmtkh sacgfdwlps
      661 elpphvkvvl tvrdgskele dlkaklknqe qcfidlgpcg vdeckdifhk slerrsrtvs
      721 akqmesisas lqqspsplla allasavlhw hgtdeptifh sedevasflf dqlkkevdpk
      781 vtslvlglls asrhglsdke itdvvssyes vlsgmphvhq krplkrcpfa vwalvhagld
      841 sflrvrvveg yvlntwsgdl yrtlcrehgp gveathvcil alfdyfqsvl taetssiils
      901 sasevdsgsq cvlpnvpkht vfsnrrkhne lpfyclhlnh lareklanvd allsrlqvan
      961 gpagdgapvk ygdsgdssea lsqivriqfl lnpewlqvkv cccdpyflle eldmflnlnp
     1021 gdtecamlre lvqlssyalr ydgrqfaahv tarlrnviat easslkfprl knlyemaqrf
     1081 pslvpvsdyl repsrkpvpa vlseeergrt lgqlytirgd ashmvslnrs dllvwdvfge
     1141 sivrrltgls eprdlkmvdr fralvlcnre lkvysldegr llvklkgvmn qkmayfglhs
     1201 qdyvvalsrn rmyvnmlnlt sgdlettfkv gedrflnsll vsgngkicvc gdetqkpfpl
     1261 lvwdlanrkl lydlriphhe fitrlsaisg dghyvvcvcr elsdsapnfi ivydlqsgtl
     1321 fkkwkperns csiaissqgg cvvngldncf vlvwdlatga rrftlkghna pvdqirldes
     1381 gfksltynsd gidrsvrvwd vmkgeclavf tpdlavtcce ltldgkavvm glegydrvfc
     1441 hllhdgegdq slpesrlasn tygnvsncgk vfdvsqqg
//
LOCUS       XP_077503662            1430 aa            linear   INV 25-NOV-2025
DEFINITION  NACHT domain- and WD repeat-containing protein 1 isoform X4
            [Amblyomma americanum].
ACCESSION   XP_077503662
VERSION     XP_077503662.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647536.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1430
                     /product="NACHT domain- and WD repeat-containing protein 1
                     isoform X4"
                     /calculated_mol_wt=158472
     Region          498..638
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            503..510
                     /site_type="other"
                     /note="Walker A motif"
                     /db_xref="CDD:99707"
     Site            order(504..511,594)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:99707"
     Site            590..595
                     /site_type="other"
                     /note="Walker B motif"
                     /db_xref="CDD:99707"
     Region          1083..1389
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          1105..1150
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1189..1229
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1234..1277
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1283..1318
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1324..1362
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1368..1410
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..1430
                     /gene="LOC144114075"
                     /coded_by="XM_077647536.1:161..4453"
                     /db_xref="GeneID:144114075"
ORIGIN      
        1 mgsscssaqk kdkerskkkk kgscspegrr rssvrstdsl pygkestppp srsdpsvtai
       61 tgstapplap vdtsltevqq nsgslaspap qvtspapgse tfsddglpsl qafpddvqal
      121 llgrlgavpa sllaagsrli slyvccglad meaerthleq hvypsvraya adkgydlniv
      181 dlhwgiqvea mdriifthlc lqtiarlrsq ghflalvlln eklenqlphi lpaslpsdvl
      241 tsvadavesp eqrqllldwy medlnsvpkc yrlhaireri pdiistnpsa kaaalelwqk
      301 essqmvealr saltaeqrak ylvsvveeel eaavlndsqk plcclwmerr fthrgpqrsn
      361 svvgtliepa egsgpvdlsd skrynlvraq ledrlpesqk lvlrvkwsre gptptenaey
      421 fdelsttlsr dlkamvdtvv eedttdettr pcrgveqqly velvqqaqtc lslseafigr
      481 aemltrleky lrsklrnpiv lhgvkgsgks sllarvaqlc etwvpeapvi arfvgtspds
      541 dtqeqllrsi cehccalygm hailasksit eqhealgvll dkvshqrpli illdgldqmt
      601 khsacgfdwl pselpphvkv vltvrdgske ledlkaklkn qeqcfidlgp cgvdeckdif
      661 hkslerrsrt vsakqmesis aslqqspspl laallasavl hwhgtdepti fhsedevasf
      721 lfdqlkkevd pkvtslvlgl lsasrhglsd keitdvvssy esvlsgmphv hqkrplkrcp
      781 favwalvhag ldsflrvrvv egyvlntwsg dlyrtlcreh gpgveathvc ilalfdyfqs
      841 vltaetssii lssasevdsg sqcvlpnvpk htvfsnrrkh nelpfyclhl nhlareklan
      901 vdallsrlqv angpagdgap vkygdsgdss ealsqivriq fllnpewlqv kvcccdpyfl
      961 leeldmflnl npgdtecaml relvqlssya lrydgrqfaa hvtarlrnvi ateasslkfp
     1021 rlknlyemaq rfpslvpvsd ylrepsrkpv pavlseeerg rtlgqlytir gdashmvsln
     1081 rsdllvwdvf gesivrrltg lseprdlkmv drfralvlcn relkvyslde grllvklkgv
     1141 mnqkmayfgl hsqdyvvals rnrmyvnmln ltsgdlettf kvgedrflns llvsgngkic
     1201 vcgdetqkpf pllvwdlanr kllydlriph hefitrlsai sgdghyvvcv crelsdsapn
     1261 fiivydlqsg tlfkkwkper nscsiaissq ggcvvngldn cfvlvwdlat garrftlkgh
     1321 napvdqirld esgfksltyn sdgidrsvrv wdvmkgecla vftpdlavtc celtldgkav
     1381 vmglegydrv fchllhdgeg dqslpesrla sntygnvsnc gkvfdvsqqg
//
LOCUS       XP_077503664             877 aa            linear   INV 25-NOV-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503664
VERSION     XP_077503664.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647538.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..877
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X1"
                     /calculated_mol_wt=96088
     Region          37..591
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          <633..822
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          764..872
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..877
                     /gene="AP-1gamma"
                     /coded_by="XM_077647538.1:46..2679"
                     /db_xref="GeneID:144114077"
ORIGIN      
        1 mdlqaalfsr lttgmptpmr lrdlirqira artaaderav vqkecayirs tfreedntwr
       61 crnvakllyi hmlgypahfg qleclklias srftdkrigy lgamlllder qdvhllitns
      121 lkndlgsqtq fvvglaltal gsicspemsr dlageverll ktsnayirkk aalsafriir
      181 kvpelmemfi patrslltek nhgvlitgvi litemceksp dtlqhfkkvr tlvpnlvril
      241 knlimagysp ehdvcgvsdp flqvkilrll rllghndpea seamndilaq vatntetskn
      301 vgnailyesv lsimdikses glrvlgvnil grfllntdkn iryvalntll rtvhadytav
      361 qrhrntildc lkdpdvsirr ralelcfali nmqniramtk ellvflekad pefkalcssn
      421 lfiaaemysp skrwhidtmi kvlttagnyv rddvvgsliq lisetsslht ytvqqlwrqv
      481 sqedfcprqp laqvacwclg efgdllnsae gsdadpvnvt edeivdfyek mlsnnqvllv
      541 tkeyavtslm klsvrfassa pqikkvvdaf ggnmnvelqq rsiefsslfs kfdhlrgsll
      601 ermppmesrs lgssggldng lareeeilld dqalsdprsp pptstgappa essalldllg
      661 gmaspaesas pppaaaqseg dvldvavaal eassppttgg sgssdlldll galdlsapap
      721 spatvpattf isslgnltap vvpdgfvsrp pvlpttngip pmtvfekdgl kldftfekep
      781 sspqtavihl tainlsscpm tdflfqaavp ktfqlqmlpp dgsvvqanss gcvkqtikvt
      841 spqknplrmr irisysinga qvleqaevnn fptsvwq
//
LOCUS       XP_077503665             877 aa            linear   INV 25-NOV-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503665
VERSION     XP_077503665.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647539.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..877
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X1"
                     /calculated_mol_wt=96088
     Region          37..591
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          <633..822
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          764..872
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..877
                     /gene="AP-1gamma"
                     /coded_by="XM_077647539.1:46..2679"
                     /db_xref="GeneID:144114077"
ORIGIN      
        1 mdlqaalfsr lttgmptpmr lrdlirqira artaaderav vqkecayirs tfreedntwr
       61 crnvakllyi hmlgypahfg qleclklias srftdkrigy lgamlllder qdvhllitns
      121 lkndlgsqtq fvvglaltal gsicspemsr dlageverll ktsnayirkk aalsafriir
      181 kvpelmemfi patrslltek nhgvlitgvi litemceksp dtlqhfkkvr tlvpnlvril
      241 knlimagysp ehdvcgvsdp flqvkilrll rllghndpea seamndilaq vatntetskn
      301 vgnailyesv lsimdikses glrvlgvnil grfllntdkn iryvalntll rtvhadytav
      361 qrhrntildc lkdpdvsirr ralelcfali nmqniramtk ellvflekad pefkalcssn
      421 lfiaaemysp skrwhidtmi kvlttagnyv rddvvgsliq lisetsslht ytvqqlwrqv
      481 sqedfcprqp laqvacwclg efgdllnsae gsdadpvnvt edeivdfyek mlsnnqvllv
      541 tkeyavtslm klsvrfassa pqikkvvdaf ggnmnvelqq rsiefsslfs kfdhlrgsll
      601 ermppmesrs lgssggldng lareeeilld dqalsdprsp pptstgappa essalldllg
      661 gmaspaesas pppaaaqseg dvldvavaal eassppttgg sgssdlldll galdlsapap
      721 spatvpattf isslgnltap vvpdgfvsrp pvlpttngip pmtvfekdgl kldftfekep
      781 sspqtavihl tainlsscpm tdflfqaavp ktfqlqmlpp dgsvvqanss gcvkqtikvt
      841 spqknplrmr irisysinga qvleqaevnn fptsvwq
//
LOCUS       XP_077503666             874 aa            linear   INV 25-NOV-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503666
VERSION     XP_077503666.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647540.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..874
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X2"
                     /calculated_mol_wt=95732
     Region          37..588
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          156..177
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          189..217
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          257..287
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          <630..819
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          761..869
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..874
                     /gene="AP-1gamma"
                     /coded_by="XM_077647540.1:45..2669"
                     /db_xref="GeneID:144114077"
ORIGIN      
        1 mdlqaalfsr lttgmptpmr lrdlirqira artaaderav vqkecayirs tfreedntwr
       61 crnvakllyi hmlgypahfg qleclklias srftdkrigy lgamlllder qdvhllitns
      121 lkndlgsqtq fvvglaltal gsicspemsr dlageverll ktsnayirkk aalsafriir
      181 kvpelmemfi patrslltek nhgvlitgvi litemceksp dtlqhfkklv pnlvrilknl
      241 imagyspehd vcgvsdpflq vkilrllrll ghndpeasea mndilaqvat ntetsknvgn
      301 ailyesvlsi mdiksesglr vlgvnilgrf llntdkniry valntllrtv hadytavqrh
      361 rntildclkd pdvsirrral elcfalinmq niramtkell vflekadpef kalcssnlfi
      421 aaemyspskr whidtmikvl ttagnyvrdd vvgsliqlis etsslhtytv qqlwrqvsqe
      481 dfcprqplaq vacwclgefg dllnsaegsd adpvnvtede ivdfyekmls nnqvllvtke
      541 yavtslmkls vrfassapqi kkvvdafggn mnvelqqrsi efsslfskfd hlrgsllerm
      601 ppmesrslgs sggldnglar eeeillddqa lsdprspppt stgappaess alldllggma
      661 spaesasppp aaaqsegdvl dvavaaleas sppttggsgs sdlldllgal dlsapapspa
      721 tvpattfiss lgnltapvvp dgfvsrppvl pttngippmt vfekdglkld ftfekepssp
      781 qtavihltai nlsscpmtdf lfqaavpktf qlqmlppdgs vvqanssgcv kqtikvtspq
      841 knplrmriri sysingaqvl eqaevnnfpt svwq
//
LOCUS       XP_077503667             874 aa            linear   INV 25-NOV-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503667
VERSION     XP_077503667.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647541.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..874
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X2"
                     /calculated_mol_wt=95732
     Region          37..588
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          156..177
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          189..217
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          257..287
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          <630..819
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          761..869
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..874
                     /gene="AP-1gamma"
                     /coded_by="XM_077647541.1:45..2669"
                     /db_xref="GeneID:144114077"
ORIGIN      
        1 mdlqaalfsr lttgmptpmr lrdlirqira artaaderav vqkecayirs tfreedntwr
       61 crnvakllyi hmlgypahfg qleclklias srftdkrigy lgamlllder qdvhllitns
      121 lkndlgsqtq fvvglaltal gsicspemsr dlageverll ktsnayirkk aalsafriir
      181 kvpelmemfi patrslltek nhgvlitgvi litemceksp dtlqhfkklv pnlvrilknl
      241 imagyspehd vcgvsdpflq vkilrllrll ghndpeasea mndilaqvat ntetsknvgn
      301 ailyesvlsi mdiksesglr vlgvnilgrf llntdkniry valntllrtv hadytavqrh
      361 rntildclkd pdvsirrral elcfalinmq niramtkell vflekadpef kalcssnlfi
      421 aaemyspskr whidtmikvl ttagnyvrdd vvgsliqlis etsslhtytv qqlwrqvsqe
      481 dfcprqplaq vacwclgefg dllnsaegsd adpvnvtede ivdfyekmls nnqvllvtke
      541 yavtslmkls vrfassapqi kkvvdafggn mnvelqqrsi efsslfskfd hlrgsllerm
      601 ppmesrslgs sggldnglar eeeillddqa lsdprspppt stgappaess alldllggma
      661 spaesasppp aaaqsegdvl dvavaaleas sppttggsgs sdlldllgal dlsapapspa
      721 tvpattfiss lgnltapvvp dgfvsrppvl pttngippmt vfekdglkld ftfekepssp
      781 qtavihltai nlsscpmtdf lfqaavpktf qlqmlppdgs vvqanssgcv kqtikvtspq
      841 knplrmriri sysingaqvl eqaevnnfpt svwq
//
LOCUS       XP_077503668             873 aa            linear   INV 25-NOV-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503668
VERSION     XP_077503668.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647542.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..873
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X3"
                     /calculated_mol_wt=95625
     Region          33..587
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          <629..818
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          760..868
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..873
                     /gene="AP-1gamma"
                     /coded_by="XM_077647542.1:97..2718"
                     /db_xref="GeneID:144114077"
ORIGIN      
        1 mpsptrlttg mptpmrlrdl irqiraarta aderavvqke cayirstfre edntwrcrnv
       61 akllyihmlg ypahfgqlec lkliassrft dkrigylgam lllderqdvh llitnslknd
      121 lgsqtqfvvg laltalgsic spemsrdlag everllktsn ayirkkaals afriirkvpe
      181 lmemfipatr sllteknhgv litgvilite mcekspdtlq hfkkvrtlvp nlvrilknli
      241 magyspehdv cgvsdpflqv kilrllrllg hndpeaseam ndilaqvatn tetsknvgna
      301 ilyesvlsim diksesglrv lgvnilgrfl lntdkniryv alntllrtvh adytavqrhr
      361 ntildclkdp dvsirrrale lcfalinmqn iramtkellv flekadpefk alcssnlfia
      421 aemyspskrw hidtmikvlt tagnyvrddv vgsliqlise tsslhtytvq qlwrqvsqed
      481 fcprqplaqv acwclgefgd llnsaegsda dpvnvtedei vdfyekmlsn nqvllvtkey
      541 avtslmklsv rfassapqik kvvdafggnm nvelqqrsie fsslfskfdh lrgsllermp
      601 pmesrslgss ggldnglare eeillddqal sdprspppts tgappaessa lldllggmas
      661 paesaspppa aaqsegdvld vavaaleass ppttggsgss dlldllgald lsapapspat
      721 vpattfissl gnltapvvpd gfvsrppvlp ttngippmtv fekdglkldf tfekepsspq
      781 tavihltain lsscpmtdfl fqaavpktfq lqmlppdgsv vqanssgcvk qtikvtspqk
      841 nplrmriris ysingaqvle qaevnnfpts vwq
//
LOCUS       XP_077503669             873 aa            linear   INV 25-NOV-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503669
VERSION     XP_077503669.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647543.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..873
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X3"
                     /calculated_mol_wt=95625
     Region          33..587
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          <629..818
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          760..868
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..873
                     /gene="AP-1gamma"
                     /coded_by="XM_077647543.1:172..2793"
                     /db_xref="GeneID:144114077"
ORIGIN      
        1 mpsptrlttg mptpmrlrdl irqiraarta aderavvqke cayirstfre edntwrcrnv
       61 akllyihmlg ypahfgqlec lkliassrft dkrigylgam lllderqdvh llitnslknd
      121 lgsqtqfvvg laltalgsic spemsrdlag everllktsn ayirkkaals afriirkvpe
      181 lmemfipatr sllteknhgv litgvilite mcekspdtlq hfkkvrtlvp nlvrilknli
      241 magyspehdv cgvsdpflqv kilrllrllg hndpeaseam ndilaqvatn tetsknvgna
      301 ilyesvlsim diksesglrv lgvnilgrfl lntdkniryv alntllrtvh adytavqrhr
      361 ntildclkdp dvsirrrale lcfalinmqn iramtkellv flekadpefk alcssnlfia
      421 aemyspskrw hidtmikvlt tagnyvrddv vgsliqlise tsslhtytvq qlwrqvsqed
      481 fcprqplaqv acwclgefgd llnsaegsda dpvnvtedei vdfyekmlsn nqvllvtkey
      541 avtslmklsv rfassapqik kvvdafggnm nvelqqrsie fsslfskfdh lrgsllermp
      601 pmesrslgss ggldnglare eeillddqal sdprspppts tgappaessa lldllggmas
      661 paesaspppa aaqsegdvld vavaaleass ppttggsgss dlldllgald lsapapspat
      721 vpattfissl gnltapvvpd gfvsrppvlp ttngippmtv fekdglkldf tfekepsspq
      781 tavihltain lsscpmtdfl fqaavpktfq lqmlppdgsv vqanssgcvk qtikvtspqk
      841 nplrmriris ysingaqvle qaevnnfpts vwq
//
LOCUS       XP_077503670             873 aa            linear   INV 25-NOV-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503670
VERSION     XP_077503670.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647544.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..873
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X3"
                     /calculated_mol_wt=95625
     Region          33..587
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          <629..818
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          760..868
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..873
                     /gene="AP-1gamma"
                     /coded_by="XM_077647544.1:186..2807"
                     /db_xref="GeneID:144114077"
ORIGIN      
        1 mpsptrlttg mptpmrlrdl irqiraarta aderavvqke cayirstfre edntwrcrnv
       61 akllyihmlg ypahfgqlec lkliassrft dkrigylgam lllderqdvh llitnslknd
      121 lgsqtqfvvg laltalgsic spemsrdlag everllktsn ayirkkaals afriirkvpe
      181 lmemfipatr sllteknhgv litgvilite mcekspdtlq hfkkvrtlvp nlvrilknli
      241 magyspehdv cgvsdpflqv kilrllrllg hndpeaseam ndilaqvatn tetsknvgna
      301 ilyesvlsim diksesglrv lgvnilgrfl lntdkniryv alntllrtvh adytavqrhr
      361 ntildclkdp dvsirrrale lcfalinmqn iramtkellv flekadpefk alcssnlfia
      421 aemyspskrw hidtmikvlt tagnyvrddv vgsliqlise tsslhtytvq qlwrqvsqed
      481 fcprqplaqv acwclgefgd llnsaegsda dpvnvtedei vdfyekmlsn nqvllvtkey
      541 avtslmklsv rfassapqik kvvdafggnm nvelqqrsie fsslfskfdh lrgsllermp
      601 pmesrslgss ggldnglare eeillddqal sdprspppts tgappaessa lldllggmas
      661 paesaspppa aaqsegdvld vavaaleass ppttggsgss dlldllgald lsapapspat
      721 vpattfissl gnltapvvpd gfvsrppvlp ttngippmtv fekdglkldf tfekepsspq
      781 tavihltain lsscpmtdfl fqaavpktfq lqmlppdgsv vqanssgcvk qtikvtspqk
      841 nplrmriris ysingaqvle qaevnnfpts vwq
//
LOCUS       XP_077503671             870 aa            linear   INV 25-NOV-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503671
VERSION     XP_077503671.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647545.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..870
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X4"
                     /calculated_mol_wt=95268
     Region          33..584
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          152..173
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(165..166,169,172..173,202..203,206,209..210,213,259,
                     272,275,278..279,292,320..321,324,340..341,373..374,377,
                     383..384)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          185..213
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          228..293
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          302..341
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          353..385
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          <626..815
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          757..865
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..870
                     /gene="AP-1gamma"
                     /coded_by="XM_077647545.1:97..2709"
                     /db_xref="GeneID:144114077"
ORIGIN      
        1 mpsptrlttg mptpmrlrdl irqiraarta aderavvqke cayirstfre edntwrcrnv
       61 akllyihmlg ypahfgqlec lkliassrft dkrigylgam lllderqdvh llitnslknd
      121 lgsqtqfvvg laltalgsic spemsrdlag everllktsn ayirkkaals afriirkvpe
      181 lmemfipatr sllteknhgv litgvilite mcekspdtlq hfkklvpnlv rilknlimag
      241 yspehdvcgv sdpflqvkil rllrllghnd peaseamndi laqvatntet sknvgnaily
      301 esvlsimdik sesglrvlgv nilgrfllnt dkniryvaln tllrtvhady tavqrhrnti
      361 ldclkdpdvs irrralelcf alinmqnira mtkellvfle kadpefkalc ssnlfiaaem
      421 yspskrwhid tmikvlttag nyvrddvvgs liqlisetss lhtytvqqlw rqvsqedfcp
      481 rqplaqvacw clgefgdlln saegsdadpv nvtedeivdf yekmlsnnqv llvtkeyavt
      541 slmklsvrfa ssapqikkvv dafggnmnve lqqrsiefss lfskfdhlrg sllermppme
      601 srslgssggl dnglareeei llddqalsdp rsppptstga ppaessalld llggmaspae
      661 saspppaaaq segdvldvav aaleassppt tggsgssdll dllgaldlsa papspatvpa
      721 ttfisslgnl tapvvpdgfv srppvlpttn gippmtvfek dglkldftfe kepsspqtav
      781 ihltainlss cpmtdflfqa avpktfqlqm lppdgsvvqa nssgcvkqti kvtspqknpl
      841 rmririsysi ngaqvleqae vnnfptsvwq
//
LOCUS       XP_077503672             870 aa            linear   INV 25-NOV-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503672
VERSION     XP_077503672.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647546.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..870
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X4"
                     /calculated_mol_wt=95268
     Region          33..584
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          152..173
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(165..166,169,172..173,202..203,206,209..210,213,259,
                     272,275,278..279,292,320..321,324,340..341,373..374,377,
                     383..384)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          185..213
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          228..293
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          302..341
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          353..385
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          <626..815
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          757..865
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..870
                     /gene="AP-1gamma"
                     /coded_by="XM_077647546.1:216..2828"
                     /db_xref="GeneID:144114077"
ORIGIN      
        1 mpsptrlttg mptpmrlrdl irqiraarta aderavvqke cayirstfre edntwrcrnv
       61 akllyihmlg ypahfgqlec lkliassrft dkrigylgam lllderqdvh llitnslknd
      121 lgsqtqfvvg laltalgsic spemsrdlag everllktsn ayirkkaals afriirkvpe
      181 lmemfipatr sllteknhgv litgvilite mcekspdtlq hfkklvpnlv rilknlimag
      241 yspehdvcgv sdpflqvkil rllrllghnd peaseamndi laqvatntet sknvgnaily
      301 esvlsimdik sesglrvlgv nilgrfllnt dkniryvaln tllrtvhady tavqrhrnti
      361 ldclkdpdvs irrralelcf alinmqnira mtkellvfle kadpefkalc ssnlfiaaem
      421 yspskrwhid tmikvlttag nyvrddvvgs liqlisetss lhtytvqqlw rqvsqedfcp
      481 rqplaqvacw clgefgdlln saegsdadpv nvtedeivdf yekmlsnnqv llvtkeyavt
      541 slmklsvrfa ssapqikkvv dafggnmnve lqqrsiefss lfskfdhlrg sllermppme
      601 srslgssggl dnglareeei llddqalsdp rsppptstga ppaessalld llggmaspae
      661 saspppaaaq segdvldvav aaleassppt tggsgssdll dllgaldlsa papspatvpa
      721 ttfisslgnl tapvvpdgfv srppvlpttn gippmtvfek dglkldftfe kepsspqtav
      781 ihltainlss cpmtdflfqa avpktfqlqm lppdgsvvqa nssgcvkqti kvtspqknpl
      841 rmririsysi ngaqvleqae vnnfptsvwq
//
LOCUS       XP_077503673             870 aa            linear   INV 25-NOV-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503673
VERSION     XP_077503673.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647547.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..870
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X4"
                     /calculated_mol_wt=95268
     Region          33..584
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          152..173
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(165..166,169,172..173,202..203,206,209..210,213,259,
                     272,275,278..279,292,320..321,324,340..341,373..374,377,
                     383..384)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          185..213
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          228..293
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          302..341
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          353..385
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          <626..815
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          757..865
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..870
                     /gene="AP-1gamma"
                     /coded_by="XM_077647547.1:189..2801"
                     /db_xref="GeneID:144114077"
ORIGIN      
        1 mpsptrlttg mptpmrlrdl irqiraarta aderavvqke cayirstfre edntwrcrnv
       61 akllyihmlg ypahfgqlec lkliassrft dkrigylgam lllderqdvh llitnslknd
      121 lgsqtqfvvg laltalgsic spemsrdlag everllktsn ayirkkaals afriirkvpe
      181 lmemfipatr sllteknhgv litgvilite mcekspdtlq hfkklvpnlv rilknlimag
      241 yspehdvcgv sdpflqvkil rllrllghnd peaseamndi laqvatntet sknvgnaily
      301 esvlsimdik sesglrvlgv nilgrfllnt dkniryvaln tllrtvhady tavqrhrnti
      361 ldclkdpdvs irrralelcf alinmqnira mtkellvfle kadpefkalc ssnlfiaaem
      421 yspskrwhid tmikvlttag nyvrddvvgs liqlisetss lhtytvqqlw rqvsqedfcp
      481 rqplaqvacw clgefgdlln saegsdadpv nvtedeivdf yekmlsnnqv llvtkeyavt
      541 slmklsvrfa ssapqikkvv dafggnmnve lqqrsiefss lfskfdhlrg sllermppme
      601 srslgssggl dnglareeei llddqalsdp rsppptstga ppaessalld llggmaspae
      661 saspppaaaq segdvldvav aaleassppt tggsgssdll dllgaldlsa papspatvpa
      721 ttfisslgnl tapvvpdgfv srppvlpttn gippmtvfek dglkldftfe kepsspqtav
      781 ihltainlss cpmtdflfqa avpktfqlqm lppdgsvvqa nssgcvkqti kvtspqknpl
      841 rmririsysi ngaqvleqae vnnfptsvwq
//
LOCUS       XP_077503674             870 aa            linear   INV 25-NOV-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503674
VERSION     XP_077503674.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647548.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..870
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X4"
                     /calculated_mol_wt=95268
     Region          33..584
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          152..173
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(165..166,169,172..173,202..203,206,209..210,213,259,
                     272,275,278..279,292,320..321,324,340..341,373..374,377,
                     383..384)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          185..213
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          228..293
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          302..341
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          353..385
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          <626..815
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          757..865
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..870
                     /gene="AP-1gamma"
                     /coded_by="XM_077647548.1:97..2709"
                     /db_xref="GeneID:144114077"
ORIGIN      
        1 mpsptrlttg mptpmrlrdl irqiraarta aderavvqke cayirstfre edntwrcrnv
       61 akllyihmlg ypahfgqlec lkliassrft dkrigylgam lllderqdvh llitnslknd
      121 lgsqtqfvvg laltalgsic spemsrdlag everllktsn ayirkkaals afriirkvpe
      181 lmemfipatr sllteknhgv litgvilite mcekspdtlq hfkklvpnlv rilknlimag
      241 yspehdvcgv sdpflqvkil rllrllghnd peaseamndi laqvatntet sknvgnaily
      301 esvlsimdik sesglrvlgv nilgrfllnt dkniryvaln tllrtvhady tavqrhrnti
      361 ldclkdpdvs irrralelcf alinmqnira mtkellvfle kadpefkalc ssnlfiaaem
      421 yspskrwhid tmikvlttag nyvrddvvgs liqlisetss lhtytvqqlw rqvsqedfcp
      481 rqplaqvacw clgefgdlln saegsdadpv nvtedeivdf yekmlsnnqv llvtkeyavt
      541 slmklsvrfa ssapqikkvv dafggnmnve lqqrsiefss lfskfdhlrg sllermppme
      601 srslgssggl dnglareeei llddqalsdp rsppptstga ppaessalld llggmaspae
      661 saspppaaaq segdvldvav aaleassppt tggsgssdll dllgaldlsa papspatvpa
      721 ttfisslgnl tapvvpdgfv srppvlpttn gippmtvfek dglkldftfe kepsspqtav
      781 ihltainlss cpmtdflfqa avpktfqlqm lppdgsvvqa nssgcvkqti kvtspqknpl
      841 rmririsysi ngaqvleqae vnnfptsvwq
//
LOCUS       XP_077503675             870 aa            linear   INV 25-NOV-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503675
VERSION     XP_077503675.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647549.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..870
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X4"
                     /calculated_mol_wt=95268
     Region          33..584
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          152..173
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(165..166,169,172..173,202..203,206,209..210,213,259,
                     272,275,278..279,292,320..321,324,340..341,373..374,377,
                     383..384)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          185..213
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          228..293
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          302..341
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          353..385
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          <626..815
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          757..865
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..870
                     /gene="AP-1gamma"
                     /coded_by="XM_077647549.1:216..2828"
                     /db_xref="GeneID:144114077"
ORIGIN      
        1 mpsptrlttg mptpmrlrdl irqiraarta aderavvqke cayirstfre edntwrcrnv
       61 akllyihmlg ypahfgqlec lkliassrft dkrigylgam lllderqdvh llitnslknd
      121 lgsqtqfvvg laltalgsic spemsrdlag everllktsn ayirkkaals afriirkvpe
      181 lmemfipatr sllteknhgv litgvilite mcekspdtlq hfkklvpnlv rilknlimag
      241 yspehdvcgv sdpflqvkil rllrllghnd peaseamndi laqvatntet sknvgnaily
      301 esvlsimdik sesglrvlgv nilgrfllnt dkniryvaln tllrtvhady tavqrhrnti
      361 ldclkdpdvs irrralelcf alinmqnira mtkellvfle kadpefkalc ssnlfiaaem
      421 yspskrwhid tmikvlttag nyvrddvvgs liqlisetss lhtytvqqlw rqvsqedfcp
      481 rqplaqvacw clgefgdlln saegsdadpv nvtedeivdf yekmlsnnqv llvtkeyavt
      541 slmklsvrfa ssapqikkvv dafggnmnve lqqrsiefss lfskfdhlrg sllermppme
      601 srslgssggl dnglareeei llddqalsdp rsppptstga ppaessalld llggmaspae
      661 saspppaaaq segdvldvav aaleassppt tggsgssdll dllgaldlsa papspatvpa
      721 ttfisslgnl tapvvpdgfv srppvlpttn gippmtvfek dglkldftfe kepsspqtav
      781 ihltainlss cpmtdflfqa avpktfqlqm lppdgsvvqa nssgcvkqti kvtspqknpl
      841 rmririsysi ngaqvleqae vnnfptsvwq
//
LOCUS       XP_077503676             863 aa            linear   INV 25-NOV-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077503676
VERSION     XP_077503676.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647550.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..863
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X5"
                     /calculated_mol_wt=94582
     Region          23..577
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          <619..808
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          750..858
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..863
                     /gene="AP-1gamma"
                     /coded_by="XM_077647550.1:149..2740"
                     /db_xref="GeneID:144114077"
ORIGIN      
        1 mptpmrlrdl irqiraarta aderavvqke cayirstfre edntwrcrnv akllyihmlg
       61 ypahfgqlec lkliassrft dkrigylgam lllderqdvh llitnslknd lgsqtqfvvg
      121 laltalgsic spemsrdlag everllktsn ayirkkaals afriirkvpe lmemfipatr
      181 sllteknhgv litgvilite mcekspdtlq hfkkvrtlvp nlvrilknli magyspehdv
      241 cgvsdpflqv kilrllrllg hndpeaseam ndilaqvatn tetsknvgna ilyesvlsim
      301 diksesglrv lgvnilgrfl lntdkniryv alntllrtvh adytavqrhr ntildclkdp
      361 dvsirrrale lcfalinmqn iramtkellv flekadpefk alcssnlfia aemyspskrw
      421 hidtmikvlt tagnyvrddv vgsliqlise tsslhtytvq qlwrqvsqed fcprqplaqv
      481 acwclgefgd llnsaegsda dpvnvtedei vdfyekmlsn nqvllvtkey avtslmklsv
      541 rfassapqik kvvdafggnm nvelqqrsie fsslfskfdh lrgsllermp pmesrslgss
      601 ggldnglare eeillddqal sdprspppts tgappaessa lldllggmas paesaspppa
      661 aaqsegdvld vavaaleass ppttggsgss dlldllgald lsapapspat vpattfissl
      721 gnltapvvpd gfvsrppvlp ttngippmtv fekdglkldf tfekepsspq tavihltain
      781 lsscpmtdfl fqaavpktfq lqmlppdgsv vqanssgcvk qtikvtspqk nplrmriris
      841 ysingaqvle qaevnnfpts vwq
//
LOCUS       XP_077503677             863 aa            linear   INV 25-NOV-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077503677
VERSION     XP_077503677.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647551.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..863
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X5"
                     /calculated_mol_wt=94582
     Region          23..577
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          <619..808
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          750..858
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..863
                     /gene="AP-1gamma"
                     /coded_by="XM_077647551.1:147..2738"
                     /db_xref="GeneID:144114077"
ORIGIN      
        1 mptpmrlrdl irqiraarta aderavvqke cayirstfre edntwrcrnv akllyihmlg
       61 ypahfgqlec lkliassrft dkrigylgam lllderqdvh llitnslknd lgsqtqfvvg
      121 laltalgsic spemsrdlag everllktsn ayirkkaals afriirkvpe lmemfipatr
      181 sllteknhgv litgvilite mcekspdtlq hfkkvrtlvp nlvrilknli magyspehdv
      241 cgvsdpflqv kilrllrllg hndpeaseam ndilaqvatn tetsknvgna ilyesvlsim
      301 diksesglrv lgvnilgrfl lntdkniryv alntllrtvh adytavqrhr ntildclkdp
      361 dvsirrrale lcfalinmqn iramtkellv flekadpefk alcssnlfia aemyspskrw
      421 hidtmikvlt tagnyvrddv vgsliqlise tsslhtytvq qlwrqvsqed fcprqplaqv
      481 acwclgefgd llnsaegsda dpvnvtedei vdfyekmlsn nqvllvtkey avtslmklsv
      541 rfassapqik kvvdafggnm nvelqqrsie fsslfskfdh lrgsllermp pmesrslgss
      601 ggldnglare eeillddqal sdprspppts tgappaessa lldllggmas paesaspppa
      661 aaqsegdvld vavaaleass ppttggsgss dlldllgald lsapapspat vpattfissl
      721 gnltapvvpd gfvsrppvlp ttngippmtv fekdglkldf tfekepsspq tavihltain
      781 lsscpmtdfl fqaavpktfq lqmlppdgsv vqanssgcvk qtikvtspqk nplrmriris
      841 ysingaqvle qaevnnfpts vwq
//
LOCUS       XP_077503678             863 aa            linear   INV 25-NOV-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077503678
VERSION     XP_077503678.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647552.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..863
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X5"
                     /calculated_mol_wt=94582
     Region          23..577
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          <619..808
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          750..858
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..863
                     /gene="AP-1gamma"
                     /coded_by="XM_077647552.1:39..2630"
                     /db_xref="GeneID:144114077"
ORIGIN      
        1 mptpmrlrdl irqiraarta aderavvqke cayirstfre edntwrcrnv akllyihmlg
       61 ypahfgqlec lkliassrft dkrigylgam lllderqdvh llitnslknd lgsqtqfvvg
      121 laltalgsic spemsrdlag everllktsn ayirkkaals afriirkvpe lmemfipatr
      181 sllteknhgv litgvilite mcekspdtlq hfkkvrtlvp nlvrilknli magyspehdv
      241 cgvsdpflqv kilrllrllg hndpeaseam ndilaqvatn tetsknvgna ilyesvlsim
      301 diksesglrv lgvnilgrfl lntdkniryv alntllrtvh adytavqrhr ntildclkdp
      361 dvsirrrale lcfalinmqn iramtkellv flekadpefk alcssnlfia aemyspskrw
      421 hidtmikvlt tagnyvrddv vgsliqlise tsslhtytvq qlwrqvsqed fcprqplaqv
      481 acwclgefgd llnsaegsda dpvnvtedei vdfyekmlsn nqvllvtkey avtslmklsv
      541 rfassapqik kvvdafggnm nvelqqrsie fsslfskfdh lrgsllermp pmesrslgss
      601 ggldnglare eeillddqal sdprspppts tgappaessa lldllggmas paesaspppa
      661 aaqsegdvld vavaaleass ppttggsgss dlldllgald lsapapspat vpattfissl
      721 gnltapvvpd gfvsrppvlp ttngippmtv fekdglkldf tfekepsspq tavihltain
      781 lsscpmtdfl fqaavpktfq lqmlppdgsv vqanssgcvk qtikvtspqk nplrmriris
      841 ysingaqvle qaevnnfpts vwq
//
LOCUS       XP_077503680             860 aa            linear   INV 25-NOV-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077503680
VERSION     XP_077503680.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647554.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..860
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X6"
                     /calculated_mol_wt=94226
     Region          23..574
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          142..163
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(155..156,159,162..163,192..193,196,199..200,203,249,
                     262,265,268..269,282,310..311,314,330..331,363..364,367,
                     373..374)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          175..203
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          218..283
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          292..331
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          343..375
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          <616..805
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          747..855
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..860
                     /gene="AP-1gamma"
                     /coded_by="XM_077647554.1:149..2731"
                     /db_xref="GeneID:144114077"
ORIGIN      
        1 mptpmrlrdl irqiraarta aderavvqke cayirstfre edntwrcrnv akllyihmlg
       61 ypahfgqlec lkliassrft dkrigylgam lllderqdvh llitnslknd lgsqtqfvvg
      121 laltalgsic spemsrdlag everllktsn ayirkkaals afriirkvpe lmemfipatr
      181 sllteknhgv litgvilite mcekspdtlq hfkklvpnlv rilknlimag yspehdvcgv
      241 sdpflqvkil rllrllghnd peaseamndi laqvatntet sknvgnaily esvlsimdik
      301 sesglrvlgv nilgrfllnt dkniryvaln tllrtvhady tavqrhrnti ldclkdpdvs
      361 irrralelcf alinmqnira mtkellvfle kadpefkalc ssnlfiaaem yspskrwhid
      421 tmikvlttag nyvrddvvgs liqlisetss lhtytvqqlw rqvsqedfcp rqplaqvacw
      481 clgefgdlln saegsdadpv nvtedeivdf yekmlsnnqv llvtkeyavt slmklsvrfa
      541 ssapqikkvv dafggnmnve lqqrsiefss lfskfdhlrg sllermppme srslgssggl
      601 dnglareeei llddqalsdp rsppptstga ppaessalld llggmaspae saspppaaaq
      661 segdvldvav aaleassppt tggsgssdll dllgaldlsa papspatvpa ttfisslgnl
      721 tapvvpdgfv srppvlpttn gippmtvfek dglkldftfe kepsspqtav ihltainlss
      781 cpmtdflfqa avpktfqlqm lppdgsvvqa nssgcvkqti kvtspqknpl rmririsysi
      841 ngaqvleqae vnnfptsvwq
//
LOCUS       XP_077503681             860 aa            linear   INV 25-NOV-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077503681
VERSION     XP_077503681.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647555.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..860
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X6"
                     /calculated_mol_wt=94226
     Region          23..574
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          142..163
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(155..156,159,162..163,192..193,196,199..200,203,249,
                     262,265,268..269,282,310..311,314,330..331,363..364,367,
                     373..374)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          175..203
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          218..283
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          292..331
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          343..375
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          <616..805
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          747..855
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..860
                     /gene="AP-1gamma"
                     /coded_by="XM_077647555.1:186..2768"
                     /db_xref="GeneID:144114077"
ORIGIN      
        1 mptpmrlrdl irqiraarta aderavvqke cayirstfre edntwrcrnv akllyihmlg
       61 ypahfgqlec lkliassrft dkrigylgam lllderqdvh llitnslknd lgsqtqfvvg
      121 laltalgsic spemsrdlag everllktsn ayirkkaals afriirkvpe lmemfipatr
      181 sllteknhgv litgvilite mcekspdtlq hfkklvpnlv rilknlimag yspehdvcgv
      241 sdpflqvkil rllrllghnd peaseamndi laqvatntet sknvgnaily esvlsimdik
      301 sesglrvlgv nilgrfllnt dkniryvaln tllrtvhady tavqrhrnti ldclkdpdvs
      361 irrralelcf alinmqnira mtkellvfle kadpefkalc ssnlfiaaem yspskrwhid
      421 tmikvlttag nyvrddvvgs liqlisetss lhtytvqqlw rqvsqedfcp rqplaqvacw
      481 clgefgdlln saegsdadpv nvtedeivdf yekmlsnnqv llvtkeyavt slmklsvrfa
      541 ssapqikkvv dafggnmnve lqqrsiefss lfskfdhlrg sllermppme srslgssggl
      601 dnglareeei llddqalsdp rsppptstga ppaessalld llggmaspae saspppaaaq
      661 segdvldvav aaleassppt tggsgssdll dllgaldlsa papspatvpa ttfisslgnl
      721 tapvvpdgfv srppvlpttn gippmtvfek dglkldftfe kepsspqtav ihltainlss
      781 cpmtdflfqa avpktfqlqm lppdgsvvqa nssgcvkqti kvtspqknpl rmririsysi
      841 ngaqvleqae vnnfptsvwq
//
LOCUS       XP_077503682             860 aa            linear   INV 25-NOV-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077503682
VERSION     XP_077503682.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647556.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..860
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X6"
                     /calculated_mol_wt=94226
     Region          23..574
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          142..163
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(155..156,159,162..163,192..193,196,199..200,203,249,
                     262,265,268..269,282,310..311,314,330..331,363..364,367,
                     373..374)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          175..203
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          218..283
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          292..331
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          343..375
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          <616..805
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          747..855
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..860
                     /gene="AP-1gamma"
                     /coded_by="XM_077647556.1:202..2784"
                     /db_xref="GeneID:144114077"
ORIGIN      
        1 mptpmrlrdl irqiraarta aderavvqke cayirstfre edntwrcrnv akllyihmlg
       61 ypahfgqlec lkliassrft dkrigylgam lllderqdvh llitnslknd lgsqtqfvvg
      121 laltalgsic spemsrdlag everllktsn ayirkkaals afriirkvpe lmemfipatr
      181 sllteknhgv litgvilite mcekspdtlq hfkklvpnlv rilknlimag yspehdvcgv
      241 sdpflqvkil rllrllghnd peaseamndi laqvatntet sknvgnaily esvlsimdik
      301 sesglrvlgv nilgrfllnt dkniryvaln tllrtvhady tavqrhrnti ldclkdpdvs
      361 irrralelcf alinmqnira mtkellvfle kadpefkalc ssnlfiaaem yspskrwhid
      421 tmikvlttag nyvrddvvgs liqlisetss lhtytvqqlw rqvsqedfcp rqplaqvacw
      481 clgefgdlln saegsdadpv nvtedeivdf yekmlsnnqv llvtkeyavt slmklsvrfa
      541 ssapqikkvv dafggnmnve lqqrsiefss lfskfdhlrg sllermppme srslgssggl
      601 dnglareeei llddqalsdp rsppptstga ppaessalld llggmaspae saspppaaaq
      661 segdvldvav aaleassppt tggsgssdll dllgaldlsa papspatvpa ttfisslgnl
      721 tapvvpdgfv srppvlpttn gippmtvfek dglkldftfe kepsspqtav ihltainlss
      781 cpmtdflfqa avpktfqlqm lppdgsvvqa nssgcvkqti kvtspqknpl rmririsysi
      841 ngaqvleqae vnnfptsvwq
//
LOCUS       XP_077503683             860 aa            linear   INV 25-NOV-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077503683
VERSION     XP_077503683.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647557.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..860
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X6"
                     /calculated_mol_wt=94226
     Region          23..574
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          142..163
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(155..156,159,162..163,192..193,196,199..200,203,249,
                     262,265,268..269,282,310..311,314,330..331,363..364,367,
                     373..374)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          175..203
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          218..283
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          292..331
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          343..375
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          <616..805
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          747..855
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..860
                     /gene="AP-1gamma"
                     /coded_by="XM_077647557.1:147..2729"
                     /db_xref="GeneID:144114077"
ORIGIN      
        1 mptpmrlrdl irqiraarta aderavvqke cayirstfre edntwrcrnv akllyihmlg
       61 ypahfgqlec lkliassrft dkrigylgam lllderqdvh llitnslknd lgsqtqfvvg
      121 laltalgsic spemsrdlag everllktsn ayirkkaals afriirkvpe lmemfipatr
      181 sllteknhgv litgvilite mcekspdtlq hfkklvpnlv rilknlimag yspehdvcgv
      241 sdpflqvkil rllrllghnd peaseamndi laqvatntet sknvgnaily esvlsimdik
      301 sesglrvlgv nilgrfllnt dkniryvaln tllrtvhady tavqrhrnti ldclkdpdvs
      361 irrralelcf alinmqnira mtkellvfle kadpefkalc ssnlfiaaem yspskrwhid
      421 tmikvlttag nyvrddvvgs liqlisetss lhtytvqqlw rqvsqedfcp rqplaqvacw
      481 clgefgdlln saegsdadpv nvtedeivdf yekmlsnnqv llvtkeyavt slmklsvrfa
      541 ssapqikkvv dafggnmnve lqqrsiefss lfskfdhlrg sllermppme srslgssggl
      601 dnglareeei llddqalsdp rsppptstga ppaessalld llggmaspae saspppaaaq
      661 segdvldvav aaleassppt tggsgssdll dllgaldlsa papspatvpa ttfisslgnl
      721 tapvvpdgfv srppvlpttn gippmtvfek dglkldftfe kepsspqtav ihltainlss
      781 cpmtdflfqa avpktfqlqm lppdgsvvqa nssgcvkqti kvtspqknpl rmririsysi
      841 ngaqvleqae vnnfptsvwq
//
LOCUS       XP_077503684             877 aa            linear   INV 25-NOV-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503684
VERSION     XP_077503684.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647558.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..877
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X1"
                     /calculated_mol_wt=96088
     Region          37..591
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          <633..822
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          764..872
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..877
                     /gene="AP-1gamma"
                     /coded_by="XM_077647558.1:46..2679"
                     /db_xref="GeneID:144114077"
ORIGIN      
        1 mdlqaalfsr lttgmptpmr lrdlirqira artaaderav vqkecayirs tfreedntwr
       61 crnvakllyi hmlgypahfg qleclklias srftdkrigy lgamlllder qdvhllitns
      121 lkndlgsqtq fvvglaltal gsicspemsr dlageverll ktsnayirkk aalsafriir
      181 kvpelmemfi patrslltek nhgvlitgvi litemceksp dtlqhfkkvr tlvpnlvril
      241 knlimagysp ehdvcgvsdp flqvkilrll rllghndpea seamndilaq vatntetskn
      301 vgnailyesv lsimdikses glrvlgvnil grfllntdkn iryvalntll rtvhadytav
      361 qrhrntildc lkdpdvsirr ralelcfali nmqniramtk ellvflekad pefkalcssn
      421 lfiaaemysp skrwhidtmi kvlttagnyv rddvvgsliq lisetsslht ytvqqlwrqv
      481 sqedfcprqp laqvacwclg efgdllnsae gsdadpvnvt edeivdfyek mlsnnqvllv
      541 tkeyavtslm klsvrfassa pqikkvvdaf ggnmnvelqq rsiefsslfs kfdhlrgsll
      601 ermppmesrs lgssggldng lareeeilld dqalsdprsp pptstgappa essalldllg
      661 gmaspaesas pppaaaqseg dvldvavaal eassppttgg sgssdlldll galdlsapap
      721 spatvpattf isslgnltap vvpdgfvsrp pvlpttngip pmtvfekdgl kldftfekep
      781 sspqtavihl tainlsscpm tdflfqaavp ktfqlqmlpp dgsvvqanss gcvkqtikvt
      841 spqknplrmr irisysinga qvleqaevnn fptsvwq
//
LOCUS       XP_077503685             641 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114079 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503685
VERSION     XP_077503685.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647559.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..641
                     /product="uncharacterized protein LOC144114079 isoform X1"
                     /calculated_mol_wt=71475
     CDS             1..641
                     /gene="LOC144114079"
                     /coded_by="XM_077647559.1:590..2515"
                     /db_xref="GeneID:144114079"
ORIGIN      
        1 madggqlrls hdellqahrk erkelqgavr crqyhlnfqq fevcfkrlqs ipfpannivs
       61 lhlheflasc alkhgiaedt lllclvttts hlmslssstk irlssawqes pvlwscvvvp
      121 rahschnfvw tlqkllcdlh cqrpsrdiap kalvvdeass tslnglltra gavlgahtsf
      181 eqvksvfsav gkgkfeklal gvpvlwssvt sevpfmsevr fnlccitdps tfhsvlssrl
      241 lessydfqec mwpnvlfaca hersldlanl heqfvdaevd rlkallssia eahrlsvqyk
      301 fseeatcvlp qllskfkqla lswncevalf ktatsqifrl aaalyvldgc lsgaiqpekr
      361 eyeipaahiw eahdivmhsl rikaefldcp lqdelfhtve npraslqsgn fqhhangtva
      421 nlgvspvfcv apemqvqsnh eqlpvpvaip relsvsivqp parenelsgl qlslsepqes
      481 yisfssvipd skeefvdkfh ykirrllisg kslltptlva qmkyvsvtdp nipgksrypt
      541 qvaalflerv aalgfgtmlt rhdshskkll frkteyhqlg paqlkllqdl ritreqysqg
      601 sqrpraalyw etgsgvpirl phspagptps lpvkqevidi n
//
LOCUS       XP_077503686             312 aa            linear   INV 25-NOV-2025
DEFINITION  deubiquitinase OTUD6B-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503686
VERSION     XP_077503686.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647560.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..312
                     /product="deubiquitinase OTUD6B-like isoform X2"
                     /calculated_mol_wt=34975
     Region          155..297
                     /region_name="OTU_OTUD6"
                     /note="OTU (ovarian tumor) domain of OTU domain-containing
                     proteins 6A and 6B and similar proteins; cd22761"
                     /db_xref="CDD:438598"
     Site            order(173,230,233,240..243,267,269,282,291..293)
                     /site_type="other"
                     /note="putative polypeptide substrate binding site
                     [polypeptide binding]"
                     /db_xref="CDD:438598"
     CDS             1..312
                     /gene="LOC144114079"
                     /coded_by="XM_077647560.1:598..1536"
                     /db_xref="GeneID:144114079"
ORIGIN      
        1 madggqlrls hdellqahrk erkelqakiq nlkhsvpkgd kkkkkdvaae vavleaelee
       61 kqkkdlqefa amqstvngnd saangqpgvn eviegldaas lhdehavmkq srvskaekrr
      121 ekkrqkevqr ekeiaaqeve nqflpravee relyeilakl glavyevpsd gncmykameh
      181 qlglfgvmks mselrqetan ymlshaeefl pfltskksgd mmtteeyeey caevsstttw
      241 ggqlelkals hackvpitvv qaygpsieig teynckpmll syhrclyemg ehynslvpne
      301 gkvdedpasl ea
//
LOCUS       XP_077503687             245 aa            linear   INV 25-NOV-2025
DEFINITION  deubiquitinase OTUD6B-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077503687
VERSION     XP_077503687.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647561.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..245
                     /product="deubiquitinase OTUD6B-like isoform X3"
                     /calculated_mol_wt=27568
     Region          13..245
                     /region_name="COG5539"
                     /note="Predicted cysteine protease (OTU family)
                     [Posttranslational modification, protein turnover,
                     chaperones]"
                     /db_xref="CDD:227826"
     Region          155..>245
                     /region_name="OTU"
                     /note="OTU (ovarian tumor) domain family; cl45892"
                     /db_xref="CDD:459237"
     CDS             1..245
                     /gene="LOC144114079"
                     /coded_by="XM_077647561.1:137..874"
                     /db_xref="GeneID:144114079"
ORIGIN      
        1 madggqlrls hdellqahrk erkelqakiq nlkhsvpkgd kkkkkdvaae vavleaelee
       61 kqkkdlqefa amqstvngnd saangqpgvn eviegldaas lhdehavmkq srvskaekrr
      121 ekkrqkevqr ekeiaaqeve nqflpravee relyeilakl glavyevpsd gncmykameh
      181 qlglfgvmks mselrqetan ymlshaeefl pfltskksgd mmtteeyeey caevsstttw
      241 ggqle
//
LOCUS       XP_077503688            1249 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114080 [Amblyomma americanum].
ACCESSION   XP_077503688
VERSION     XP_077503688.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647562.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1249
                     /product="uncharacterized protein LOC144114080"
                     /calculated_mol_wt=143026
     Region          1087..1135
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cd00167"
                     /db_xref="CDD:238096"
     CDS             1..1249
                     /gene="LOC144114080"
                     /coded_by="XM_077647562.1:607..4356"
                     /db_xref="GeneID:144114080"
ORIGIN      
        1 mprhfdlnqg yeevqprkkr vmgslddnsv yeadsedcah resrrrkkrs sactstpace
       61 vgeaftdesd tvgkecleke pislfrecay esktekkkky qrrkkcesat ddncayaese
      121 qiaeksysnl shitqvsddt rearhrhkky kkgktlrdsd sddneiedvp cvvrseqtdv
      181 kkedqsvael eecgnidemv lerkkrkykk pfdtedrysn lnhrtqisdd ivdarhkhkk
      241 hkkvetlrds esagneiedv pcvmqyeqtd vkkedqsvae leecgnidem vlerkkrknk
      301 kpfdtedrys nlnhrteisd divdarrkhk khkkvetlrd sesagneied vpcvmqyeqt
      361 dvekedqsva eleecgnide mvlerkkrkn kkpfdtedry snlkhhtqis ddivdarhkh
      421 kkvetlrdse svgneiedvp cvmqyeqtdv ekedqsvael eecgnidemv lerkkrknkk
      481 pfdtedrysn lnhrtqisdd ivgarhkhkk hkkvetlrds esagneiedv pcvmqyeqtd
      541 vekedqsvae leecgkmdnm gvkrkkrkkk kpcdaeyssc rdeidllfsd enlsstpgrv
      601 hkkkkykatv erlqadsgsa vsdlqencad khstfsgesd rilsddgnqn cnlrtphkrk
      661 krevakeyfq egascsfsdq qmdgcgntet crhvddghcc stgavhkkkp cqlklaaeca
      721 dcatdcalsc pteelcdnas figvggenaq mgselenqse tslgtslrel gesplkrkgs
      781 shcitqehsg tqqdlgiafk kkkrklarls afgdelfctp slqadadsse tagkcskgde
      841 mdapeiydtv pstdvgsank klklhdrstp gtsnetlssa gsrkytehac rsllrklrgh
      901 rrstspsdpd fvaylngeqr ypyihhktkp sldailryqe dtgltvemgk ytpeedfill
      961 kniheivkyy digyphmivg hcgdqdpamq kevqdfvrke qlirvlgrrl pcrtihsaym
     1021 rarilldpla srkkvvlnrq qkkrlqrmyd clgpkwtvma kkmglsaeqc rsafrhvkek
     1081 ktfatgkwqq sedellrqav erqtggrsvl lkdinwqavs krvgsrnasq crrrwtliql
     1141 tpppgqkark wgrfdsihli yimhklnask sakvdweavg ksfpwasspl iakyhwegtl
     1201 nyylplkqrd nfqkkvknly elalpsllkk ygkgktleei ielskshta
//
LOCUS       XP_077503689            1249 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114080 [Amblyomma americanum].
ACCESSION   XP_077503689
VERSION     XP_077503689.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647563.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1249
                     /product="uncharacterized protein LOC144114080"
                     /calculated_mol_wt=143026
     Region          1087..1135
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cd00167"
                     /db_xref="CDD:238096"
     CDS             1..1249
                     /gene="LOC144114080"
                     /coded_by="XM_077647563.1:607..4356"
                     /db_xref="GeneID:144114080"
ORIGIN      
        1 mprhfdlnqg yeevqprkkr vmgslddnsv yeadsedcah resrrrkkrs sactstpace
       61 vgeaftdesd tvgkecleke pislfrecay esktekkkky qrrkkcesat ddncayaese
      121 qiaeksysnl shitqvsddt rearhrhkky kkgktlrdsd sddneiedvp cvvrseqtdv
      181 kkedqsvael eecgnidemv lerkkrkykk pfdtedrysn lnhrtqisdd ivdarhkhkk
      241 hkkvetlrds esagneiedv pcvmqyeqtd vkkedqsvae leecgnidem vlerkkrknk
      301 kpfdtedrys nlnhrteisd divdarrkhk khkkvetlrd sesagneied vpcvmqyeqt
      361 dvekedqsva eleecgnide mvlerkkrkn kkpfdtedry snlkhhtqis ddivdarhkh
      421 kkvetlrdse svgneiedvp cvmqyeqtdv ekedqsvael eecgnidemv lerkkrknkk
      481 pfdtedrysn lnhrtqisdd ivgarhkhkk hkkvetlrds esagneiedv pcvmqyeqtd
      541 vekedqsvae leecgkmdnm gvkrkkrkkk kpcdaeyssc rdeidllfsd enlsstpgrv
      601 hkkkkykatv erlqadsgsa vsdlqencad khstfsgesd rilsddgnqn cnlrtphkrk
      661 krevakeyfq egascsfsdq qmdgcgntet crhvddghcc stgavhkkkp cqlklaaeca
      721 dcatdcalsc pteelcdnas figvggenaq mgselenqse tslgtslrel gesplkrkgs
      781 shcitqehsg tqqdlgiafk kkkrklarls afgdelfctp slqadadsse tagkcskgde
      841 mdapeiydtv pstdvgsank klklhdrstp gtsnetlssa gsrkytehac rsllrklrgh
      901 rrstspsdpd fvaylngeqr ypyihhktkp sldailryqe dtgltvemgk ytpeedfill
      961 kniheivkyy digyphmivg hcgdqdpamq kevqdfvrke qlirvlgrrl pcrtihsaym
     1021 rarilldpla srkkvvlnrq qkkrlqrmyd clgpkwtvma kkmglsaeqc rsafrhvkek
     1081 ktfatgkwqq sedellrqav erqtggrsvl lkdinwqavs krvgsrnasq crrrwtliql
     1141 tpppgqkark wgrfdsihli yimhklnask sakvdweavg ksfpwasspl iakyhwegtl
     1201 nyylplkqrd nfqkkvknly elalpsllkk ygkgktleei ielskshta
//
LOCUS       XP_077503690            1249 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114080 [Amblyomma americanum].
ACCESSION   XP_077503690
VERSION     XP_077503690.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647564.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1249
                     /product="uncharacterized protein LOC144114080"
                     /calculated_mol_wt=143026
     Region          1087..1135
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cd00167"
                     /db_xref="CDD:238096"
     CDS             1..1249
                     /gene="LOC144114080"
                     /coded_by="XM_077647564.1:607..4356"
                     /db_xref="GeneID:144114080"
ORIGIN      
        1 mprhfdlnqg yeevqprkkr vmgslddnsv yeadsedcah resrrrkkrs sactstpace
       61 vgeaftdesd tvgkecleke pislfrecay esktekkkky qrrkkcesat ddncayaese
      121 qiaeksysnl shitqvsddt rearhrhkky kkgktlrdsd sddneiedvp cvvrseqtdv
      181 kkedqsvael eecgnidemv lerkkrkykk pfdtedrysn lnhrtqisdd ivdarhkhkk
      241 hkkvetlrds esagneiedv pcvmqyeqtd vkkedqsvae leecgnidem vlerkkrknk
      301 kpfdtedrys nlnhrteisd divdarrkhk khkkvetlrd sesagneied vpcvmqyeqt
      361 dvekedqsva eleecgnide mvlerkkrkn kkpfdtedry snlkhhtqis ddivdarhkh
      421 kkvetlrdse svgneiedvp cvmqyeqtdv ekedqsvael eecgnidemv lerkkrknkk
      481 pfdtedrysn lnhrtqisdd ivgarhkhkk hkkvetlrds esagneiedv pcvmqyeqtd
      541 vekedqsvae leecgkmdnm gvkrkkrkkk kpcdaeyssc rdeidllfsd enlsstpgrv
      601 hkkkkykatv erlqadsgsa vsdlqencad khstfsgesd rilsddgnqn cnlrtphkrk
      661 krevakeyfq egascsfsdq qmdgcgntet crhvddghcc stgavhkkkp cqlklaaeca
      721 dcatdcalsc pteelcdnas figvggenaq mgselenqse tslgtslrel gesplkrkgs
      781 shcitqehsg tqqdlgiafk kkkrklarls afgdelfctp slqadadsse tagkcskgde
      841 mdapeiydtv pstdvgsank klklhdrstp gtsnetlssa gsrkytehac rsllrklrgh
      901 rrstspsdpd fvaylngeqr ypyihhktkp sldailryqe dtgltvemgk ytpeedfill
      961 kniheivkyy digyphmivg hcgdqdpamq kevqdfvrke qlirvlgrrl pcrtihsaym
     1021 rarilldpla srkkvvlnrq qkkrlqrmyd clgpkwtvma kkmglsaeqc rsafrhvkek
     1081 ktfatgkwqq sedellrqav erqtggrsvl lkdinwqavs krvgsrnasq crrrwtliql
     1141 tpppgqkark wgrfdsihli yimhklnask sakvdweavg ksfpwasspl iakyhwegtl
     1201 nyylplkqrd nfqkkvknly elalpsllkk ygkgktleei ielskshta
//
LOCUS       XP_077503691            1037 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114082 [Amblyomma americanum].
ACCESSION   XP_077503691
VERSION     XP_077503691.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647565.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1037
                     /product="uncharacterized protein LOC144114082"
                     /calculated_mol_wt=114891
     Region          891..1008
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     CDS             1..1037
                     /gene="LOC144114082"
                     /coded_by="XM_077647565.1:354..3467"
                     /db_xref="GeneID:144114082"
ORIGIN      
        1 mdgqktpvlk kipswlrsfr iskgdasepq nkevrpascq pkgflrsyrr slvskkneep
       61 gvesnhgtqd fargslmsir kiwaeeakaq aydertkser rpkkscalrt klldspskip
      121 isermhvhqr elqlsfhpsn vssnkenlak vfapkcknhe nqssagssdg nlaseivlsa
      181 arhaknrrsl vrehdvwrta vnnvglprts tmleqlpktg keqdlptlsa vvraakndkd
      241 kriqnwvmrn sgcgnglsar htmfcvqqsq lphrqsveet ipkyidcapk ipprtylakl
      301 ahakahlakt iapvtharap ttthningak cslsghrdal cnkvehvcll sskkaaikqr
      361 iscerhrrkc eevvhstenp sklqqprkam rpqvqhnpsy gtgctsrmqn lmkncrasgs
      421 dvmaddeatv lncscdrclv kfsaagkhmp ekmgdlhndc yrgvqrhtin scfskygkha
      481 savsqhckcc qfkktcatlk vvvdtrgesl nteascekis ekaaqcstst khafhcqdei
      541 tkvievavka siekllssns ipqltkswsa eslheptqfk kasssplnyi plsschsgta
      601 nallnsrdga tvdsdivlvh qpcptgeten eindddddif ktcsqsphse fclerstslp
      661 slvessspak lqvstafikl vkrvnsgqdn deissqrtsf ldgtgnlgsl tlgescsecs
      721 ssdtegcwqp aqshctddlc vrpektsvqm aqrdadtsrs lsgkhgqlep kifqseqqls
      781 leldesrfsl asipgrmppd gwertppcda pvgadlddee lselnpcmse dvqencssds
      841 dgsddqhile evirkgmgvt ppiklplrcp klvkmprkfk paemfcgkai lklsayvnsg
      901 hvtvhviras klvtsrggsa nayikvslqp dhikrahwrt avvkssrnpe fdhkfsfelm
      961 aedtgkrllv tvwhrdfenk rsellgsmsf gmgkilnsnq yvkgwyrllr qdlgmrrhfa
     1021 arcsrgsctr vktsvsd
//
LOCUS       XP_077503692             236 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: protein obstructor-E-like [Amblyomma
            americanum].
ACCESSION   XP_077503692
VERSION     XP_077503692.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647566.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 2 indels
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..236
                     /product="LOW QUALITY PROTEIN: protein obstructor-E-like"
                     /calculated_mol_wt=26419
     Region          16..57
                     /region_name="ChtBD2"
                     /note="Chitin-binding domain type 2; smart00494"
                     /db_xref="CDD:214696"
     Region          93..139
                     /region_name="CBM_14"
                     /note="Chitin binding Peritrophin-A domain; pfam01607"
                     /db_xref="CDD:426342"
     Region          165..214
                     /region_name="CBM_14"
                     /note="Chitin binding Peritrophin-A domain; pfam01607"
                     /db_xref="CDD:426342"
     CDS             1..236
                     /gene="LOC144114083"
                     /coded_by="XM_077647566.1:269..979"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 2 bases in 2 codons"
                     /db_xref="GeneID:144114083"
ORIGIN      
        1 mllyatlifv gllglgrcqc ttngffphet qcdsyyeckn gtvvqgfcpd glvfndeasy
       61 khlrcdlpfd incqnrpylq paqgvgncpr rwgmyadetn cgkfyncvdg ksfpfdcpeg
      121 laynerrgvc dwpdxvercd aeaylgfqcp eptayelqdf vnppyahprd cakhfvcvst
      181 yygkrlprlx scdegtvfnp strtcdepvn vpgcenyyga qenpfnkgqt lrrqgr
//
LOCUS       XP_077503695             227 aa            linear   INV 25-NOV-2025
DEFINITION  copper transporter 1A [Amblyomma americanum].
ACCESSION   XP_077503695
VERSION     XP_077503695.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647569.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..227
                     /product="copper transporter 1A"
                     /calculated_mol_wt=25013
     Region          83..212
                     /region_name="Ctr"
                     /note="Ctr copper transporter family; pfam04145"
                     /db_xref="CDD:461194"
     CDS             1..227
                     /gene="Ctr1A"
                     /coded_by="XM_077647569.1:113..796"
                     /db_xref="GeneID:144114084"
ORIGIN      
        1 mshgsdhsgh hvhgghgehg alplnmttps phsstplqtv itaalaavtm stpvhhsshg
       61 sssqhdhhmd gsshgsmdmh mamyfhtgfs etilfkdwav ssvagmvgsv vgvffmaaly
      121 ealkyfrehl frlhfssmsy ssvsvmgqdg rtltevhqiv rnrivswphl lqtflhmvqm
      181 vvsyflmlif mtynvwlclg vilgagfgyf vfgwkkatvv dvtdhch
//
LOCUS       XP_077503696             227 aa            linear   INV 25-NOV-2025
DEFINITION  copper transporter 1A [Amblyomma americanum].
ACCESSION   XP_077503696
VERSION     XP_077503696.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647570.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..227
                     /product="copper transporter 1A"
                     /calculated_mol_wt=25013
     Region          83..212
                     /region_name="Ctr"
                     /note="Ctr copper transporter family; pfam04145"
                     /db_xref="CDD:461194"
     CDS             1..227
                     /gene="Ctr1A"
                     /coded_by="XM_077647570.1:153..836"
                     /db_xref="GeneID:144114084"
ORIGIN      
        1 mshgsdhsgh hvhgghgehg alplnmttps phsstplqtv itaalaavtm stpvhhsshg
       61 sssqhdhhmd gsshgsmdmh mamyfhtgfs etilfkdwav ssvagmvgsv vgvffmaaly
      121 ealkyfrehl frlhfssmsy ssvsvmgqdg rtltevhqiv rnrivswphl lqtflhmvqm
      181 vvsyflmlif mtynvwlclg vilgagfgyf vfgwkkatvv dvtdhch
//
LOCUS       XP_077503697             402 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114085 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503697
VERSION     XP_077503697.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647571.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..402
                     /product="uncharacterized protein LOC144114085 isoform X1"
                     /calculated_mol_wt=45339
     Region          151..256
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..402
                     /gene="LOC144114085"
                     /coded_by="XM_077647571.1:142..1350"
                     /db_xref="GeneID:144114085"
ORIGIN      
        1 mafdrkqevp dedsskssds ryvseltesf serafrsall tgllllvlla vvhaivfcsk
       61 encfraltty slsdylaysf tpsdefwiaa fygmgmftat fltvylnsai pgvepplpwd
      121 rnmhpihhsv rrakraeale krfrsykded vvyvdaaeyg kntmtvavvn nkqllkisas
      181 ilsgnpetae eaaialacvg tkaryiisds kvailnfsrg rvspltntil srhvidrkit
      241 lvwtpahasl pgnetaheft rgfsfradps evvftdrdrm vtyreitqhy rlqrarlppa
      301 drslsryqai nwrrlqtytf pnpalwslmy pgqfsekcan celratldhi vwecphapre
      361 grgintkdqw etlllssdpa tqlqlvrlae aaaesqdhsa vv
//
LOCUS       XP_077503698             178 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114085 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503698
VERSION     XP_077503698.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647572.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..178
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..178
                     /product="uncharacterized protein LOC144114085 isoform X2"
                     /calculated_mol_wt=19664
     CDS             1..178
                     /gene="LOC144114085"
                     /coded_by="XM_077647572.1:142..678"
                     /db_xref="GeneID:144114085"
ORIGIN      
        1 mafdrkqevp dedsskssds ryvseltesf serafrsall tgllllvlla vvhaivfcsk
       61 encfraltty slsdylaysf tpsdefwiaa fygmgmftat fltvylnsai pgvepplpwd
      121 rsggtitvsy amcplvglmt fwwchgalpl flstviplsl ptdlediwgs rclvllps
//
LOCUS       XP_077503699             157 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114085 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503699
VERSION     XP_077503699.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647573.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..157
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..157
                     /product="uncharacterized protein LOC144114085 isoform X3"
                     /calculated_mol_wt=17447
     CDS             1..157
                     /gene="LOC144114085"
                     /coded_by="XM_077647573.1:142..615"
                     /db_xref="GeneID:144114085"
ORIGIN      
        1 mafdrkqevp dedsskssds ryvseltesf serafrsall tgllllvlla vvhaivfcsk
       61 encfraltty slsdylaysf tpsdefwiaa fygmgmftat fltvylnsai pgvepplpwd
      121 rwchgalplf lstviplslp tdlediwgsr clvllps
//
LOCUS       XP_077503700             147 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114085 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503700
VERSION     XP_077503700.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647574.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..147
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..147
                     /product="uncharacterized protein LOC144114085 isoform X4"
                     /calculated_mol_wt=16284
     CDS             1..147
                     /gene="LOC144114085"
                     /coded_by="XM_077647574.1:142..585"
                     /db_xref="GeneID:144114085"
ORIGIN      
        1 mafdrkqevp dedsskssds ryvseltesf serafrsall tgllllvlla vvhaivfcsk
       61 encfraltty slsdylaysf tpsdefwiaa fygmgmftat fltvylnsai pgvepplpwd
      121 rsggtitvsy amcplvglmt fwctlft
//
LOCUS       XP_077503701             147 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114086 [Amblyomma americanum].
ACCESSION   XP_077503701
VERSION     XP_077503701.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647575.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..147
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..147
                     /product="uncharacterized protein LOC144114086"
                     /calculated_mol_wt=16211
     CDS             1..147
                     /gene="LOC144114086"
                     /coded_by="XM_077647575.1:162..605"
                     /db_xref="GeneID:144114086"
ORIGIN      
        1 mafdrkqevp dedsskssds gyvseltesf serafrsall tgllllvlla vvhaivfcsk
       61 encfraltty slsdylaylf tpsdefwiaa fygmgmftat fltvylnsai pgvepplpwd
      121 rsggtitvsy amcplvglmt fwctlft
//
LOCUS       XP_077503702             218 aa            linear   INV 25-NOV-2025
DEFINITION  salivary anticoagulant protein P23-like [Amblyomma americanum].
ACCESSION   XP_077503702
VERSION     XP_077503702.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647576.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..218
                     /product="salivary anticoagulant protein P23-like"
                     /calculated_mol_wt=23573
     CDS             1..218
                     /gene="LOC144114087"
                     /coded_by="XM_077647576.1:118..774"
                     /db_xref="GeneID:144114087"
ORIGIN      
        1 maitfitlsl lvvgalagsk qdannymdav lrdhlpanvr slnldplqip gfnvkvdstg
       61 ltnrdvegkf pkgvlyglss avrrrgdcgv pgwqlssvtt gcyvsldtlr ltydgnykgf
      121 nilgskknis ldlvvektna fieatgapgq aatlktltvs giefrvnfnk klelnekrek
      181 kfvkavkraa egilqgvlyt sfrealsrsv grvplprp
//
LOCUS       XP_077503703             112 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114089 [Amblyomma americanum].
ACCESSION   XP_077503703
VERSION     XP_077503703.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647577.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..112
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..112
                     /product="uncharacterized protein LOC144114089"
                     /calculated_mol_wt=11739
     CDS             1..112
                     /gene="LOC144114089"
                     /coded_by="XM_077647577.1:80..418"
                     /db_xref="GeneID:144114089"
ORIGIN      
        1 maravtfapl lalsalamcs aqrnrfnfnf qpppprhrfs vqaqgagqnr rnfnvggsvq
       61 geydvyrgkg gsrvvvsggv ahgatrvdgk tykgkpegnv gigveipigk gr
//
LOCUS       XP_077503704             267 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114090 [Amblyomma americanum].
ACCESSION   XP_077503704
VERSION     XP_077503704.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647578.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..267
                     /product="uncharacterized protein LOC144114090"
                     /calculated_mol_wt=30739
     CDS             1..267
                     /gene="LOC144114090"
                     /coded_by="XM_077647578.1:76..879"
                     /db_xref="GeneID:144114090"
ORIGIN      
        1 mrnmvstyaa vcvlatvlal aaadgkagav skgvqpagkp aaakpavvvv krpeapkqpe
       61 ydywrpwyty nspqqhhrgh arkeshhppp kqpqphsqpp rqqhpqrqap pqnlfnwpsn
      121 sggfqwgrgd dkvkdsyvke vkpvaydtvi eeekkvvvke epkvvvkevp kvvvteppkv
      181 ivqevpkkek vmvkeckdhh hdhdsmskff kkmekdfkkf nkqfkdhhkh hqkshgkaiv
      241 ffrtqtpyyy ytrapryqad nyyyngy
//
LOCUS       XP_077503705             139 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114091 [Amblyomma americanum].
ACCESSION   XP_077503705
VERSION     XP_077503705.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647579.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..139
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..139
                     /product="uncharacterized protein LOC144114091"
                     /calculated_mol_wt=15273
     CDS             1..139
                     /gene="LOC144114091"
                     /coded_by="XM_077647579.1:247..666"
                     /db_xref="GeneID:144114091"
ORIGIN      
        1 msstqtnsyh vrlrtvlgia fcfaltsetg satvlrqymn ilppmkevdp artprlsmvv
       61 sqpryfycvl ypwsvvvspv ycmrqyrkyq eylrrrgpst vkpddlgydp glidpraasv
      121 sfglpggqps aaggqagsv
//
LOCUS       XP_077503707             328 aa            linear   INV 25-NOV-2025
DEFINITION  band 7 protein AGAP004871-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503707
VERSION     XP_077503707.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647581.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..328
                     /product="band 7 protein AGAP004871-like isoform X1"
                     /calculated_mol_wt=36029
     Region          108..309
                     /region_name="SPFH_like"
                     /note="core domain of the SPFH (stomatin, prohibitin,
                     flotillin, and HflK/C) superfamily; cl19107"
                     /db_xref="CDD:473137"
     CDS             1..328
                     /gene="LOC144114092"
                     /coded_by="XM_077647581.1:278..1264"
                     /db_xref="GeneID:144114092"
ORIGIN      
        1 mtpdnpgssp afsegdeggg grrvagarrv fkfrnlrsla rtqlgravnr arelresaea
       61 glglcgwilt alsfvivvvt fpfsllfcik vvqeyeravi frlgrllqgg skgpgiffil
      121 pcienytkvd lrtltfdvpp qevltkdsvt vsvdavvyyr vhnaavsvan venahhstrl
      181 laqttlrnil gtrnlheila dreqisnamq saldectdaw gikverveik dvrlpvqlqr
      241 amaaeaeaar earakliaae geqkssralk eaadvmslsp aalqlrylqt lntisaekns
      301 tiifplpidf lacfmpdkkk hramdpdp
//
LOCUS       XP_077503708             290 aa            linear   INV 25-NOV-2025
DEFINITION  band 7 protein AGAP004871-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503708
VERSION     XP_077503708.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647582.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..290
                     /product="band 7 protein AGAP004871-like isoform X2"
                     /calculated_mol_wt=31763
     Region          70..271
                     /region_name="SPFH_like"
                     /note="core domain of the SPFH (stomatin, prohibitin,
                     flotillin, and HflK/C) superfamily; cl19107"
                     /db_xref="CDD:473137"
     CDS             1..290
                     /gene="LOC144114092"
                     /coded_by="XM_077647582.1:191..1063"
                     /db_xref="GeneID:144114092"
ORIGIN      
        1 mpkngdhvkv pltgaigesa eaglglcgwi ltalsfvivv vtfpfsllfc ikvvqeyera
       61 vifrlgrllq ggskgpgiff ilpcienytk vdlrtltfdv ppqevltkds vtvsvdavvy
      121 yrvhnaavsv anvenahhst rllaqttlrn ilgtrnlhei ladreqisna mqsaldectd
      181 awgikverve ikdvrlpvql qramaaeaea arearaklia aegeqkssra lkeaadvmsl
      241 spaalqlryl qtlntisaek nstiifplpi dflacfmpdk kkhramdpdp
//
LOCUS       XP_077503709             634 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase TRIM45 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503709
VERSION     XP_077503709.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647583.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..634
                     /product="E3 ubiquitin-protein ligase TRIM45 isoform X1"
                     /calculated_mol_wt=70250
     Region          25..70
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Region          233..264
                     /region_name="zf-B_box"
                     /note="B-box zinc finger; pfam00643"
                     /db_xref="CDD:459886"
     Region          283..388
                     /region_name="CC_brat-like"
                     /note="coiled-coil (CC) domain of Drosophila brain tumor
                     (brat) and similar proteins; cl29238"
                     /db_xref="CDD:475168"
     Site            order(283..284,286..288,291,294,308..309,312,315..316,
                     319..320,323,330..331,334,337..338,341..342,345,355..356,
                     359,363,366..367,370..371,373..374,382,386..387)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:467844"
     Region          <522..546
                     /region_name="IG_FLMN"
                     /note="Filamin-type immunoglobulin domains; smart00557"
                     /db_xref="CDD:214720"
     CDS             1..634
                     /gene="LOC144114093"
                     /coded_by="XM_077647583.1:103..2007"
                     /db_xref="GeneID:144114093"
ORIGIN      
        1 mqseqargnr frykaphrld rldlqcpacl eklqdprilp clhtlcrrci erlsrcqrrl
       61 lssrpsaary dtaraictre earnalsate swnnrgpggh rcddrceert gagvivcptc
      121 qnecvvsatq nelpenvlvr qflgpapdpl gswqhsglkq pasrgksssl cdlcaksraa
      181 vnrcigclvs cctrvrrrqp ggdsrstwsp aqkrlergdq nartmapldl kacplhpmhq
      241 mdifcvtcrr ticasclsrd htdhvssdis takekvlkrt gllltrlqsk msklensang
      301 tlkktqalsr nasevthevn sfydgyvsal earrrellde itnfqqvsqk slrdrsstle
      361 aalvrarsvh efgktllhfg hempevvlpl vevldraarp ivvmdekedi sacqplslrf
      421 rrdkedhrge ylvygavsrq adpsvvsavl sfkdpansqr fgstkaarlk fldssghpld
      481 vgpeqvraal fstksaskte aevtravddp lqlrlefdtk nagthalavt lddqpvlgsp
      541 flfkvksrkp rcagnralva ssperssayh adkgflpqsa skrqrnpgdd dfkasldkal
      601 weglskpclq pnlsgsdvms nassrssrly qfta
//
LOCUS       XP_077503710             632 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase TRIM45 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503710
VERSION     XP_077503710.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647584.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..632
                     /product="E3 ubiquitin-protein ligase TRIM45 isoform X2"
                     /calculated_mol_wt=69992
     Region          23..68
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Region          231..262
                     /region_name="zf-B_box"
                     /note="B-box zinc finger; pfam00643"
                     /db_xref="CDD:459886"
     Region          281..386
                     /region_name="CC_brat-like"
                     /note="coiled-coil (CC) domain of Drosophila brain tumor
                     (brat) and similar proteins; cl29238"
                     /db_xref="CDD:475168"
     Site            order(281..282,284..286,289,292,306..307,310,313..314,
                     317..318,321,328..329,332,335..336,339..340,343,353..354,
                     357,361,364..365,368..369,371..372,380,384..385)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:467844"
     Region          <520..544
                     /region_name="IG_FLMN"
                     /note="Filamin-type immunoglobulin domains; smart00557"
                     /db_xref="CDD:214720"
     CDS             1..632
                     /gene="LOC144114093"
                     /coded_by="XM_077647584.1:184..2082"
                     /db_xref="GeneID:144114093"
ORIGIN      
        1 msrpannefr ykaphrldrl dlqcpaclek lqdprilpcl htlcrrcier lsrcqrrlls
       61 srpsaarydt araictreea rnalsatesw nnrgpgghrc ddrceertga gvivcptcqn
      121 ecvvsatqne lpenvlvrqf lgpapdplgs wqhsglkqpa srgkssslcd lcaksraavn
      181 rcigclvscc trvrrrqpgg dsrstwspaq krlergdqna rtmapldlka cplhpmhqmd
      241 ifcvtcrrti casclsrdht dhvssdista kekvlkrtgl lltrlqskms klensangtl
      301 kktqalsrna sevthevnsf ydgyvsalea rrrelldeit nfqqvsqksl rdrsstleaa
      361 lvrarsvhef gktllhfghe mpevvlplve vldraarpiv vmdekedisa cqplslrfrr
      421 dkedhrgeyl vygavsrqad psvvsavlsf kdpansqrfg stkaarlkfl dssghpldvg
      481 peqvraalfs tksaskteae vtravddplq lrlefdtkna gthalavtld dqpvlgspfl
      541 fkvksrkprc agnralvass perssayhad kgflpqsask rqrnpgdddf kasldkalwe
      601 glskpclqpn lsgsdvmsna ssrssrlyqf ta
//
LOCUS       XP_077503711             184 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114094 [Amblyomma americanum].
ACCESSION   XP_077503711
VERSION     XP_077503711.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647585.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..184
                     /product="uncharacterized protein LOC144114094"
                     /calculated_mol_wt=19925
     CDS             1..184
                     /gene="LOC144114094"
                     /coded_by="XM_077647585.1:145..699"
                     /db_xref="GeneID:144114094"
ORIGIN      
        1 msrgrpfqsa allrkedaar ifcrniweql grngiygeic wlaeqdmvhn ealalflqvq
       61 pslgcaeegf taalpyqpdi ksfarasrcp vieivqpffv stsatpsvts shvdipaair
      121 scppaistsg lgsstsdmml nqfcfivqma lvlwcslvqh nsdclcvssl aailkesnif
      181 hgps
//
LOCUS       XP_077503712             556 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: man(5)GlcNAc(2)-PP-dolichol translocation
            protein RFT1-like [Amblyomma americanum].
ACCESSION   XP_077503712
VERSION     XP_077503712.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647586.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..556
                     /product="LOW QUALITY PROTEIN: man(5)GlcNAc(2)-PP-dolichol
                     translocation protein RFT1-like"
                     /calculated_mol_wt=61884
     Region          11..540
                     /region_name="MATE_like"
                     /note="Multidrug and toxic compound extrusion family and
                     similar proteins; cl09326"
                     /db_xref="CDD:447704"
     CDS             1..556
                     /gene="LOC144114100"
                     /coded_by="XM_077647586.1:351..2021"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:144114100"
ORIGIN      
        1 magsnlmana traasynivl qltlrvltfv lnayilrhit rdllgvinvr lmllyttvqf
       61 lsrepfrrsc lsdtdnqnwp aiinltwlcl pvcvfigamm sfvwlfvveq pdpsvaggyv
      121 lgvhcvvisv vievlaeply vvsqafhyik fkvffvgsgi alrciimagl vafdpenavr
      181 aysiaqliss vyytvvifay ftfesrrlrq ltksdsqdsg lnqddhalpf ttaldiipfi
      241 gcngtqldhd vtkltwsfmk qtvfkqllte geryimtvfs ilsfaeqgvy dvvnnlgslt
      301 arlvfepvge ssyiffaqvv qrdvppdrqd vdsitlsast lkyllkllth igliilifgq
      361 aysglllhiy ggsalsgsla plllrwhcay ivliaingvt ecfvfaamnk eqldqhnqrl
      421 tlfsvlfllv sylltslfga vgfilaicfn miarigysmf fitayyaktq ycplhgmlps
      481 gtvlgasvfs yvvtkvseaa fcceagftyl lfhtavgamc lgtllaviyi qekeltaflk
      541 tcwhekslgk qrekvq
//
LOCUS       XP_077503713             178 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114102 [Amblyomma americanum].
ACCESSION   XP_077503713
VERSION     XP_077503713.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647587.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..178
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..178
                     /product="uncharacterized protein LOC144114102"
                     /calculated_mol_wt=19537
     CDS             1..178
                     /gene="LOC144114102"
                     /coded_by="XM_077647587.1:318..854"
                     /db_xref="GeneID:144114102"
ORIGIN      
        1 mkphrpcsle lqqqwnhtvm fpagqpgsev ktnhlamleq cevtapvngr agqrgkrvgh
       61 spsrrlarpg rrlrprrphr hrgegchlvl lasghaaard hrlarnpavd esgargclqv
      121 seeaehlvdl rldrleagsr glrsaidlda frsaaevppv pafvaprllw ymavafdg
//
LOCUS       XP_077503714             293 aa            linear   INV 25-NOV-2025
DEFINITION  Golgi-associated plant pathogenesis-related protein 1-like isoform
            X1 [Amblyomma americanum].
ACCESSION   XP_077503714
VERSION     XP_077503714.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647588.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..293
                     /product="Golgi-associated plant pathogenesis-related
                     protein 1-like isoform X1"
                     /calculated_mol_wt=32494
     Region          82..>126
                     /region_name="Jag_EloR"
                     /note="RNA-binding cell elongation regulator Jag/EloR;
                     NF041568"
                     /db_xref="CDD:469453"
     Region          147..279
                     /region_name="CAP_GAPR1-like"
                     /note="CAP (cysteine-rich secretory proteins, antigen 5,
                     and pathogenesis-related 1 proteins) domain of
                     Golgi-associated plant pathogenesis-related protein 1 and
                     similar proteins; cd05382"
                     /db_xref="CDD:349401"
     CDS             1..293
                     /gene="LOC144114106"
                     /coded_by="XM_077647588.1:484..1365"
                     /db_xref="GeneID:144114106"
ORIGIN      
        1 mgqccrkeap rggqmssgnq sfqqtvtrvd ggvvttstrt tqriiqvset sgypsdqfpt
       61 ghhvkrtvvt rtikqhadgt eevtedvvtd dgvprvqdkp kkglfgflgg rkdktrvtie
      121 eapsdqqats rlisdrkapt kplsdkefrq dslkwhnhyr aihgvpplkh sdelcryaqe
      181 wadtlakkdt fchrpnnkyg eniymawssd ptkevtgrea vdswyseiqq hqfgceprsl
      241 gsghftqvvw kastelgtar artatgkllv vanynpagnl igsfaqnvpp pkk
//
LOCUS       XP_077503715             285 aa            linear   INV 25-NOV-2025
DEFINITION  Golgi-associated plant pathogenesis-related protein 1-like isoform
            X2 [Amblyomma americanum].
ACCESSION   XP_077503715
VERSION     XP_077503715.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647589.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..285
                     /product="Golgi-associated plant pathogenesis-related
                     protein 1-like isoform X2"
                     /calculated_mol_wt=31706
     Region          74..>118
                     /region_name="Jag_EloR"
                     /note="RNA-binding cell elongation regulator Jag/EloR;
                     NF041568"
                     /db_xref="CDD:469453"
     Region          139..271
                     /region_name="CAP_GAPR1-like"
                     /note="CAP (cysteine-rich secretory proteins, antigen 5,
                     and pathogenesis-related 1 proteins) domain of
                     Golgi-associated plant pathogenesis-related protein 1 and
                     similar proteins; cd05382"
                     /db_xref="CDD:349401"
     CDS             1..285
                     /gene="LOC144114106"
                     /coded_by="XM_077647589.1:1735..2592"
                     /db_xref="GeneID:144114106"
ORIGIN      
        1 mqrvaemssg nqsfqqtvtr vdggvvttst rttqriiqvs etsgypsdqf ptghhvkrtv
       61 vtrtikqhad gteevtedvv tddgvprvqd kpkkglfgfl ggrkdktrvt ieeapsdqqa
      121 tsrlisdrka ptkplsdkef rqdslkwhnh yraihgvppl khsdelcrya qewadtlakk
      181 dtfchrpnnk ygeniymaws sdptkevtgr eavdswysei qqhqfgcepr slgsghftqv
      241 vwkastelgt arartatgkl lvvanynpag nligsfaqnv pppkk
//
LOCUS       XP_077503716             279 aa            linear   INV 25-NOV-2025
DEFINITION  Golgi-associated plant pathogenesis-related protein 1-like isoform
            X3 [Amblyomma americanum].
ACCESSION   XP_077503716
VERSION     XP_077503716.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647590.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..279
                     /product="Golgi-associated plant pathogenesis-related
                     protein 1-like isoform X3"
                     /calculated_mol_wt=30992
     Region          68..>112
                     /region_name="Jag_EloR"
                     /note="RNA-binding cell elongation regulator Jag/EloR;
                     NF041568"
                     /db_xref="CDD:469453"
     Region          133..265
                     /region_name="CAP_GAPR1-like"
                     /note="CAP (cysteine-rich secretory proteins, antigen 5,
                     and pathogenesis-related 1 proteins) domain of
                     Golgi-associated plant pathogenesis-related protein 1 and
                     similar proteins; cd05382"
                     /db_xref="CDD:349401"
     CDS             1..279
                     /gene="LOC144114106"
                     /coded_by="XM_077647590.1:142..981"
                     /db_xref="GeneID:144114106"
ORIGIN      
        1 mssgnqsfqq tvtrvdggvv ttstrttqri iqvsetsgyp sdqfptghhv krtvvtrtik
       61 qhadgteevt edvvtddgvp rvqdkpkkgl fgflggrkdk trvtieeaps dqqatsrlis
      121 drkaptkpls dkefrqdslk whnhyraihg vpplkhsdel cryaqewadt lakkdtfchr
      181 pnnkygeniy mawssdptke vtgreavdsw yseiqqhqfg ceprslgsgh ftqvvwkast
      241 elgtararta tgkllvvany npagnligsf aqnvpppkk
//
LOCUS       XP_077503717             279 aa            linear   INV 25-NOV-2025
DEFINITION  Golgi-associated plant pathogenesis-related protein 1-like isoform
            X3 [Amblyomma americanum].
ACCESSION   XP_077503717
VERSION     XP_077503717.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647591.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..279
                     /product="Golgi-associated plant pathogenesis-related
                     protein 1-like isoform X3"
                     /calculated_mol_wt=30992
     Region          68..>112
                     /region_name="Jag_EloR"
                     /note="RNA-binding cell elongation regulator Jag/EloR;
                     NF041568"
                     /db_xref="CDD:469453"
     Region          133..265
                     /region_name="CAP_GAPR1-like"
                     /note="CAP (cysteine-rich secretory proteins, antigen 5,
                     and pathogenesis-related 1 proteins) domain of
                     Golgi-associated plant pathogenesis-related protein 1 and
                     similar proteins; cd05382"
                     /db_xref="CDD:349401"
     CDS             1..279
                     /gene="LOC144114106"
                     /coded_by="XM_077647591.1:166..1005"
                     /db_xref="GeneID:144114106"
ORIGIN      
        1 mssgnqsfqq tvtrvdggvv ttstrttqri iqvsetsgyp sdqfptghhv krtvvtrtik
       61 qhadgteevt edvvtddgvp rvqdkpkkgl fgflggrkdk trvtieeaps dqqatsrlis
      121 drkaptkpls dkefrqdslk whnhyraihg vpplkhsdel cryaqewadt lakkdtfchr
      181 pnnkygeniy mawssdptke vtgreavdsw yseiqqhqfg ceprslgsgh ftqvvwkast
      241 elgtararta tgkllvvany npagnligsf aqnvpppkk
//
LOCUS       XP_077503718             270 aa            linear   INV 25-NOV-2025
DEFINITION  Golgi-associated plant pathogenesis-related protein 1-like
            [Amblyomma americanum].
ACCESSION   XP_077503718
VERSION     XP_077503718.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647592.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..270
                     /product="Golgi-associated plant pathogenesis-related
                     protein 1-like"
                     /calculated_mol_wt=29830
     Region          123..255
                     /region_name="CAP_GAPR1-like"
                     /note="CAP (cysteine-rich secretory proteins, antigen 5,
                     and pathogenesis-related 1 proteins) domain of
                     Golgi-associated plant pathogenesis-related protein 1 and
                     similar proteins; cd05382"
                     /db_xref="CDD:349401"
     CDS             1..270
                     /gene="LOC144114107"
                     /coded_by="XM_077647592.1:117..929"
                     /db_xref="GeneID:144114107"
ORIGIN      
        1 mssgsqsfqq tvtrvdggvr tttttttqrv vqvsetsgyp gdrlqtghhv qrtvvtrttk
       61 ehasgtaevi edvvtndgvl riqdkpkkgl fgflggkkdk trvtpvdaps drkaptkpls
      121 dkefrketlk whnhyrtihg vpplkhsdel cryaqewadt lakkdtfchr pnnkygeniy
      181 vawssdptke vtgrevvdsw ysevqkhqfg ceprslgsgh ftqvvwkast elgtararta
      241 tgkllvvany npagnligsf aqnvpppkks
//
LOCUS       XP_077503719             178 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114108 [Amblyomma americanum].
ACCESSION   XP_077503719
VERSION     XP_077503719.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647593.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..178
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..178
                     /product="uncharacterized protein LOC144114108"
                     /calculated_mol_wt=19651
     CDS             1..178
                     /gene="LOC144114108"
                     /coded_by="XM_077647593.1:4168..4704"
                     /db_xref="GeneID:144114108"
ORIGIN      
        1 mkprrpysle lqqqwnhtim fqagqpgsev ktnhlamleq cevtapvngr agqrgkrvgh
       61 spsrrlarrg rrlrprrphr rrgegshlvl lasghaaard hrlardaald esgargclqv
      121 seeaehlvdl rldlleagsr gfcsaidlda frsapevpsv ptfvvprlll ymavafdg
//
LOCUS       XP_077503720             245 aa            linear   INV 25-NOV-2025
DEFINITION  Golgi-associated plant pathogenesis-related protein 1-like
            [Amblyomma americanum].
ACCESSION   XP_077503720
VERSION     XP_077503720.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647594.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..245
                     /product="Golgi-associated plant pathogenesis-related
                     protein 1-like"
                     /calculated_mol_wt=27591
     Region          96..230
                     /region_name="CAP_GAPR1-like"
                     /note="CAP (cysteine-rich secretory proteins, antigen 5,
                     and pathogenesis-related 1 proteins) domain of
                     Golgi-associated plant pathogenesis-related protein 1 and
                     similar proteins; cd05382"
                     /db_xref="CDD:349401"
     CDS             1..245
                     /gene="LOC144114109"
                     /coded_by="XM_077647594.1:97..834"
                     /db_xref="GeneID:144114109"
ORIGIN      
        1 mgdflrqvad tlawcctcrr ppatgphedf ptttifknvs yddhgrrivq tktvqssllr
       61 kkivhrpdgt kdvveeiitp gghamlardr pmteaeflkd sldwhnyyrs lhgvpplaad
      121 qklcemakkw atflakedrf ehspdtdhge nvyvkwssna khqitgreav eswyseiady
      181 rwdgsspdid avghftqliw ketthmgcaq argpthkilv vanysppgnv igkfaacvpp
      241 rlyam
//
LOCUS       XP_077503721             114 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114110 [Amblyomma americanum].
ACCESSION   XP_077503721
VERSION     XP_077503721.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647595.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..114
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..114
                     /product="uncharacterized protein LOC144114110"
                     /calculated_mol_wt=12396
     Region          <1..>27
                     /region_name="CAP"
                     /note="CAP (cysteine-rich secretory proteins, antigen 5,
                     and pathogenesis-related 1 proteins) domain family;
                     cl00133"
                     /db_xref="CDD:412178"
     CDS             1..114
                     /gene="LOC144114110"
                     /coded_by="XM_077647595.1:155..499"
                     /db_xref="GeneID:144114110"
ORIGIN      
        1 mawssdptke vtgreavdsw yseiqqhqlg ceprslanrr ragrpadvpt spagswphtp
       61 lpraqlmpga cpcvrrclfv rgsgsfghth hdegvpylgn lgktqcikln crcs
//
LOCUS       XP_077503722             494 aa            linear   INV 25-NOV-2025
DEFINITION  pre-mRNA processing factor 19 [Amblyomma americanum].
ACCESSION   XP_077503722
VERSION     XP_077503722.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647596.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..494
                     /product="pre-mRNA processing factor 19"
                     /calculated_mol_wt=53340
     Region          3..56
                     /region_name="RING-Ubox_PRP19"
                     /note="U-box domain, a modified RING finger, found in
                     pre-mRNA-processing factor 19 (Prp19) and similar
                     proteins; cd16656"
                     /db_xref="CDD:438318"
     Site            order(3,15,17,21..22,24,53..56)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:438318"
     Region          69..133
                     /region_name="Prp19"
                     /note="Prp19/Pso4-like; pfam08606"
                     /db_xref="CDD:400774"
     Region          <198..491
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          218..254
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          260..296
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          301..337
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          343..378
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          384..423
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          427..461
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          467..491
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..494
                     /gene="Prp19"
                     /coded_by="XM_077647596.1:157..1641"
                     /db_xref="GeneID:144114111"
ORIGIN      
        1 msfccalsne tpeqpvvspa sgsvferrli skyvrehgtd pvngqpltee mlidlkvapi
       61 akprppsats vpallkalqd ewdavmlhsf slrqqlqtar qelshtlyqh daacrviarl
      121 tkevtaarea latlkpqaag ggaaagavet vametteeag gmtaevvrrl qekaalltaq
      181 rkkrgkaipe dlakpeelra yqtlgshpgl hsaglpgila ldvspsdril tggsdrnaav
      241 fnkdteqvva vlkghskkvt svvfhpdedt avtaspdttv rvwhipsaqt tqiirahegp
      301 vtglslhatg dyllststdq hwafsdlhtg rvlarvpdns altaaefhpd glilgtgtse
      361 slvkiwdlke rtnvanfpgh tgsitalafs engyylataa gdaavklwdl rklknfrtiv
      421 ledgyevrdl ffdqsgtyla vagtdvrlyl ckqwdtlkvf sdhtalatgv rfthrarnva
      481 svsmdrtlki ygtp
//
LOCUS       XP_077503723             494 aa            linear   INV 25-NOV-2025
DEFINITION  pre-mRNA processing factor 19 [Amblyomma americanum].
ACCESSION   XP_077503723
VERSION     XP_077503723.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647597.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..494
                     /product="pre-mRNA processing factor 19"
                     /calculated_mol_wt=53340
     Region          3..56
                     /region_name="RING-Ubox_PRP19"
                     /note="U-box domain, a modified RING finger, found in
                     pre-mRNA-processing factor 19 (Prp19) and similar
                     proteins; cd16656"
                     /db_xref="CDD:438318"
     Site            order(3,15,17,21..22,24,53..56)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:438318"
     Region          69..133
                     /region_name="Prp19"
                     /note="Prp19/Pso4-like; pfam08606"
                     /db_xref="CDD:400774"
     Region          <198..491
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          218..254
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          260..296
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          301..337
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          343..378
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          384..423
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          427..461
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          467..491
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..494
                     /gene="Prp19"
                     /coded_by="XM_077647597.1:210..1694"
                     /db_xref="GeneID:144114111"
ORIGIN      
        1 msfccalsne tpeqpvvspa sgsvferrli skyvrehgtd pvngqpltee mlidlkvapi
       61 akprppsats vpallkalqd ewdavmlhsf slrqqlqtar qelshtlyqh daacrviarl
      121 tkevtaarea latlkpqaag ggaaagavet vametteeag gmtaevvrrl qekaalltaq
      181 rkkrgkaipe dlakpeelra yqtlgshpgl hsaglpgila ldvspsdril tggsdrnaav
      241 fnkdteqvva vlkghskkvt svvfhpdedt avtaspdttv rvwhipsaqt tqiirahegp
      301 vtglslhatg dyllststdq hwafsdlhtg rvlarvpdns altaaefhpd glilgtgtse
      361 slvkiwdlke rtnvanfpgh tgsitalafs engyylataa gdaavklwdl rklknfrtiv
      421 ledgyevrdl ffdqsgtyla vagtdvrlyl ckqwdtlkvf sdhtalatgv rfthrarnva
      481 svsmdrtlki ygtp
//
LOCUS       XP_077503724             900 aa            linear   INV 25-NOV-2025
DEFINITION  prolyl endopeptidase FAP-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503724
VERSION     XP_077503724.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647598.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..900
                     /product="prolyl endopeptidase FAP-like isoform X1"
                     /calculated_mol_wt=99906
     Region          212..582
                     /region_name="DPPIV_N"
                     /note="Dipeptidyl peptidase IV (DPP IV) N-terminal region;
                     pfam00930"
                     /db_xref="CDD:395744"
     Region          665..885
                     /region_name="Peptidase_S9"
                     /note="Prolyl oligopeptidase family; pfam00326"
                     /db_xref="CDD:459761"
     CDS             1..900
                     /gene="LOC144114112"
                     /coded_by="XM_077647598.1:180..2882"
                     /db_xref="GeneID:144114112"
ORIGIN      
        1 mdvgetgcfg sekqgggrer dkrhrspdar vpaarwltrd ssdldaagte lriplleqgd
       61 tdpaedmelv ssapdsrnwr gigiallvia gvcalivtav illtpgptgn alstglrkai
      121 egladnyivr rvldqegdkg prvkqarisl eevlhgtfnp rkfngswvsa tdfvyrdadg
      181 alvlynaqnr ekrtllhntt frqydvrkyy mspdlnflll vhdivpvfrh sftakykiyd
      241 fsnrevfplk hssnqeelqy aawgqtgsql tyvfennlyl ipsvgdsspv qltntgvpgv
      301 vfngvadwly eeevlgssga lwwsphgsrl cyatfndsav stmplplyeg qypavlplry
      361 pkagtanpev elrvaavshg gaetaklvqp pqelldktse yyvtmaafld eeallvnfls
      421 raqnwtlvsv creaasawhc skqavetasg gwielseppl vtpplkggpa fflrlpvqda
      481 hrgnfrhlaa fqdkrrtwlt hgpydtiaal afrpktlhlf yrstregrpg eshvyqvpfd
      541 kegaqpvclt chlgdqclyn nawfspeaef yvleclgpgv pwvalystad hkqlemlesn
      601 aelkelvenr ampqvrnlva plpdgynatv rlllppglrd eevlkypflv nvyggpgsqq
      661 vtelfrinwg hylasrkgiv yglvdgrgsg lqgdrrlhqv yrrlgtveve dqlrvarhlk
      721 nefsfisseh taiwgwsygg yvsamalang gpgnngndtp hggaeggavf qcaisvapvs
      781 nwlyydsayt erymglprdn lagyeradlt raaarlkgkk fllvhgtadd nvhfqhsmml
      841 akeltrkgvl yktqvypdek halshvtthl ynamedfidd cfegsdhave evglmqvkas
//
LOCUS       XP_077503725             899 aa            linear   INV 25-NOV-2025
DEFINITION  prolyl endopeptidase FAP-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503725
VERSION     XP_077503725.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647599.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..899
                     /product="prolyl endopeptidase FAP-like isoform X2"
                     /calculated_mol_wt=99849
     Region          211..581
                     /region_name="DPPIV_N"
                     /note="Dipeptidyl peptidase IV (DPP IV) N-terminal region;
                     pfam00930"
                     /db_xref="CDD:395744"
     Region          664..884
                     /region_name="Peptidase_S9"
                     /note="Prolyl oligopeptidase family; pfam00326"
                     /db_xref="CDD:459761"
     CDS             1..899
                     /gene="LOC144114112"
                     /coded_by="XM_077647599.1:180..2879"
                     /db_xref="GeneID:144114112"
ORIGIN      
        1 mdvgetgcfg sekqgggrer dkrhrspdar vpaarwltrd ssdldaagte lriplleqgd
       61 tdpaedmelv ssapdsrnwr gigiallvia gvcalivtav illtpgptgn alstglrkai
      121 egladnyivr rvldqedkgp rvkqarisle evlhgtfnpr kfngswvsat dfvyrdadga
      181 lvlynaqnre krtllhnttf rqydvrkyym spdlnflllv hdivpvfrhs ftakykiydf
      241 snrevfplkh ssnqeelqya awgqtgsqlt yvfennlyli psvgdsspvq ltntgvpgvv
      301 fngvadwlye eevlgssgal wwsphgsrlc yatfndsavs tmplplyegq ypavlplryp
      361 kagtanpeve lrvaavshgg aetaklvqpp qelldktsey yvtmaaflde eallvnflsr
      421 aqnwtlvsvc reaasawhcs kqavetasgg wielsepplv tpplkggpaf flrlpvqdah
      481 rgnfrhlaaf qdkrrtwlth gpydtiaala frpktlhlfy rstregrpge shvyqvpfdk
      541 egaqpvcltc hlgdqclynn awfspeaefy vleclgpgvp wvalystadh kqlemlesna
      601 elkelvenra mpqvrnlvap lpdgynatvr lllppglrde evlkypflvn vyggpgsqqv
      661 telfrinwgh ylasrkgivy glvdgrgsgl qgdrrlhqvy rrlgtveved qlrvarhlkn
      721 efsfisseht aiwgwsyggy vsamalangg pgnngndtph ggaeggavfq caisvapvsn
      781 wlyydsayte rymglprdnl agyeradltr aaarlkgkkf llvhgtaddn vhfqhsmmla
      841 keltrkgvly ktqvypdekh alshvtthly namedfiddc fegsdhavee vglmqvkas
//
LOCUS       XP_077503726             888 aa            linear   INV 25-NOV-2025
DEFINITION  prolyl endopeptidase FAP-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077503726
VERSION     XP_077503726.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647600.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..888
                     /product="prolyl endopeptidase FAP-like isoform X3"
                     /calculated_mol_wt=98405
     Region          200..570
                     /region_name="DPPIV_N"
                     /note="Dipeptidyl peptidase IV (DPP IV) N-terminal region;
                     pfam00930"
                     /db_xref="CDD:395744"
     Region          653..873
                     /region_name="Peptidase_S9"
                     /note="Prolyl oligopeptidase family; pfam00326"
                     /db_xref="CDD:459761"
     CDS             1..888
                     /gene="LOC144114112"
                     /coded_by="XM_077647600.1:179..2845"
                     /db_xref="GeneID:144114112"
ORIGIN      
        1 mdvgetgcfg sekqgggrer dkrhrspdar vpaarwltrd ssdldaagte lriplleqgd
       61 tdpaedmelv ssapdsrnwr gigiallvia gvcalivtav illtpgptgn alstglrkai
      121 eglagdkgpr vkqarislee vlhgtfnprk fngswvsatd fvyrdadgal vlynaqnrek
      181 rtllhnttfr qydvrkyyms pdlnflllvh divpvfrhsf takykiydfs nrevfplkhs
      241 snqeelqyaa wgqtgsqlty vfennlylip svgdsspvql tntgvpgvvf ngvadwlyee
      301 evlgssgalw wsphgsrlcy atfndsavst mplplyegqy pavlplrypk agtanpevel
      361 rvaavshgga etaklvqppq elldktseyy vtmaafldee allvnflsra qnwtlvsvcr
      421 eaasawhcsk qavetasggw ielsepplvt pplkggpaff lrlpvqdahr gnfrhlaafq
      481 dkrrtwlthg pydtiaalaf rpktlhlfyr stregrpges hvyqvpfdke gaqpvcltch
      541 lgdqclynna wfspeaefyv leclgpgvpw valystadhk qlemlesnae lkelvenram
      601 pqvrnlvapl pdgynatvrl llppglrdee vlkypflvnv yggpgsqqvt elfrinwghy
      661 lasrkgivyg lvdgrgsglq gdrrlhqvyr rlgtvevedq lrvarhlkne fsfissehta
      721 iwgwsyggyv samalanggp gnngndtphg gaeggavfqc aisvapvsnw lyydsayter
      781 ymglprdnla gyeradltra aarlkgkkfl lvhgtaddnv hfqhsmmlak eltrkgvlyk
      841 tqvypdekha lshvtthlyn amedfiddcf egsdhaveev glmqvkas
//
LOCUS       XP_077503727             875 aa            linear   INV 25-NOV-2025
DEFINITION  prolyl endopeptidase FAP-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077503727
VERSION     XP_077503727.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647601.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..875
                     /product="prolyl endopeptidase FAP-like isoform X4"
                     /calculated_mol_wt=96948
     Region          187..557
                     /region_name="DPPIV_N"
                     /note="Dipeptidyl peptidase IV (DPP IV) N-terminal region;
                     pfam00930"
                     /db_xref="CDD:395744"
     Region          640..860
                     /region_name="Peptidase_S9"
                     /note="Prolyl oligopeptidase family; pfam00326"
                     /db_xref="CDD:459761"
     CDS             1..875
                     /gene="LOC144114112"
                     /coded_by="XM_077647601.1:789..3416"
                     /db_xref="GeneID:144114112"
ORIGIN      
        1 mnysggtssv pgkvqggler ksttgsakrt spqdptlpeq fselvssapd srnwrgigia
       61 llviagvcal ivtavilltp gptgnalstg lrkaieglad nyivrrvldq egdkgprvkq
      121 arisleevlh gtfnprkfng swvsatdfvy rdadgalvly naqnrekrtl lhnttfrqyd
      181 vrkyymspdl nflllvhdiv pvfrhsftak ykiydfsnre vfplkhssnq eelqyaawgq
      241 tgsqltyvfe nnlylipsvg dsspvqltnt gvpgvvfngv adwlyeeevl gssgalwwsp
      301 hgsrlcyatf ndsavstmpl plyegqypav lplrypkagt anpevelrva avshggaeta
      361 klvqppqell dktseyyvtm aafldeeall vnflsraqnw tlvsvcreaa sawhcskqav
      421 etasggwiel sepplvtppl kggpafflrl pvqdahrgnf rhlaafqdkr rtwlthgpyd
      481 tiaalafrpk tlhlfyrstr egrpgeshvy qvpfdkegaq pvcltchlgd qclynnawfs
      541 peaefyvlec lgpgvpwval ystadhkqle mlesnaelke lvenrampqv rnlvaplpdg
      601 ynatvrlllp pglrdeevlk ypflvnvygg pgsqqvtelf rinwghylas rkgivyglvd
      661 grgsglqgdr rlhqvyrrlg tvevedqlrv arhlknefsf issehtaiwg wsyggyvsam
      721 alanggpgnn gndtphggae ggavfqcais vapvsnwlyy dsayterymg lprdnlagye
      781 radltraaar lkgkkfllvh gtaddnvhfq hsmmlakelt rkgvlyktqv ypdekhalsh
      841 vtthlyname dfiddcfegs dhaveevglm qvkas
//
LOCUS       XP_077503728             869 aa            linear   INV 25-NOV-2025
DEFINITION  prolyl endopeptidase FAP-like isoform X5 [Amblyomma americanum].
ACCESSION   XP_077503728
VERSION     XP_077503728.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647602.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..869
                     /product="prolyl endopeptidase FAP-like isoform X5"
                     /calculated_mol_wt=96596
     Region          181..551
                     /region_name="DPPIV_N"
                     /note="Dipeptidyl peptidase IV (DPP IV) N-terminal region;
                     pfam00930"
                     /db_xref="CDD:395744"
     Region          634..854
                     /region_name="Peptidase_S9"
                     /note="Prolyl oligopeptidase family; pfam00326"
                     /db_xref="CDD:459761"
     CDS             1..869
                     /gene="LOC144114112"
                     /coded_by="XM_077647602.1:177..2786"
                     /db_xref="GeneID:144114112"
ORIGIN      
        1 mdvgetgcfg sekqgggrer dkrhrspdar vpaarwltrd ssdldaagte lriplleqgd
       61 tdpaedmelv ssapdsrnwr gigiallvia gvcalivtav illtpgdkgp rvkqarisle
      121 evlhgtfnpr kfngswvsat dfvyrdadga lvlynaqnre krtllhnttf rqydvrkyym
      181 spdlnflllv hdivpvfrhs ftakykiydf snrevfplkh ssnqeelqya awgqtgsqlt
      241 yvfennlyli psvgdsspvq ltntgvpgvv fngvadwlye eevlgssgal wwsphgsrlc
      301 yatfndsavs tmplplyegq ypavlplryp kagtanpeve lrvaavshgg aetaklvqpp
      361 qelldktsey yvtmaaflde eallvnflsr aqnwtlvsvc reaasawhcs kqavetasgg
      421 wielsepplv tpplkggpaf flrlpvqdah rgnfrhlaaf qdkrrtwlth gpydtiaala
      481 frpktlhlfy rstregrpge shvyqvpfdk egaqpvcltc hlgdqclynn awfspeaefy
      541 vleclgpgvp wvalystadh kqlemlesna elkelvenra mpqvrnlvap lpdgynatvr
      601 lllppglrde evlkypflvn vyggpgsqqv telfrinwgh ylasrkgivy glvdgrgsgl
      661 qgdrrlhqvy rrlgtveved qlrvarhlkn efsfisseht aiwgwsyggy vsamalangg
      721 pgnngndtph ggaeggavfq caisvapvsn wlyydsayte rymglprdnl agyeradltr
      781 aaarlkgkkf llvhgtaddn vhfqhsmmla keltrkgvly ktqvypdekh alshvtthly
      841 namedfiddc fegsdhavee vglmqvkas
//
LOCUS       XP_077503729             868 aa            linear   INV 25-NOV-2025
DEFINITION  inactive dipeptidyl peptidase 10-like isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077503729
VERSION     XP_077503729.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647603.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..868
                     /product="inactive dipeptidyl peptidase 10-like isoform
                     X6"
                     /calculated_mol_wt=96539
     Region          180..550
                     /region_name="DPPIV_N"
                     /note="Dipeptidyl peptidase IV (DPP IV) N-terminal region;
                     pfam00930"
                     /db_xref="CDD:395744"
     Region          633..853
                     /region_name="Peptidase_S9"
                     /note="Prolyl oligopeptidase family; pfam00326"
                     /db_xref="CDD:459761"
     CDS             1..868
                     /gene="LOC144114112"
                     /coded_by="XM_077647603.1:176..2782"
                     /db_xref="GeneID:144114112"
ORIGIN      
        1 mdvgetgcfg sekqgggrer dkrhrspdar vpaarwltrd ssdldaagte lriplleqgd
       61 tdpaedmelv ssapdsrnwr gigiallvia gvcalivtav illtpdkgpr vkqarislee
      121 vlhgtfnprk fngswvsatd fvyrdadgal vlynaqnrek rtllhnttfr qydvrkyyms
      181 pdlnflllvh divpvfrhsf takykiydfs nrevfplkhs snqeelqyaa wgqtgsqlty
      241 vfennlylip svgdsspvql tntgvpgvvf ngvadwlyee evlgssgalw wsphgsrlcy
      301 atfndsavst mplplyegqy pavlplrypk agtanpevel rvaavshgga etaklvqppq
      361 elldktseyy vtmaafldee allvnflsra qnwtlvsvcr eaasawhcsk qavetasggw
      421 ielsepplvt pplkggpaff lrlpvqdahr gnfrhlaafq dkrrtwlthg pydtiaalaf
      481 rpktlhlfyr stregrpges hvyqvpfdke gaqpvcltch lgdqclynna wfspeaefyv
      541 leclgpgvpw valystadhk qlemlesnae lkelvenram pqvrnlvapl pdgynatvrl
      601 llppglrdee vlkypflvnv yggpgsqqvt elfrinwghy lasrkgivyg lvdgrgsglq
      661 gdrrlhqvyr rlgtvevedq lrvarhlkne fsfissehta iwgwsyggyv samalanggp
      721 gnngndtphg gaeggavfqc aisvapvsnw lyydsayter ymglprdnla gyeradltra
      781 aarlkgkkfl lvhgtaddnv hfqhsmmlak eltrkgvlyk tqvypdekha lshvtthlyn
      841 amedfiddcf egsdhaveev glmqvkas
//
LOCUS       XP_077503730             855 aa            linear   INV 25-NOV-2025
DEFINITION  prolyl endopeptidase FAP-like isoform X7 [Amblyomma americanum].
ACCESSION   XP_077503730
VERSION     XP_077503730.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647604.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..855
                     /product="prolyl endopeptidase FAP-like isoform X7"
                     /calculated_mol_wt=94844
     Region          167..537
                     /region_name="DPPIV_N"
                     /note="Dipeptidyl peptidase IV (DPP IV) N-terminal region;
                     pfam00930"
                     /db_xref="CDD:395744"
     Region          620..840
                     /region_name="Peptidase_S9"
                     /note="Prolyl oligopeptidase family; pfam00326"
                     /db_xref="CDD:459761"
     CDS             1..855
                     /gene="LOC144114112"
                     /coded_by="XM_077647604.1:58..2625"
                     /db_xref="GeneID:144114112"
ORIGIN      
        1 mtssaepkkk ptaapvpqdp naelvssapd srnwrgigia llviagvcal ivtavilltp
       61 gptgnalstg lrkaieglad nyivrrvldq egdkgprvkq arisleevlh gtfnprkfng
      121 swvsatdfvy rdadgalvly naqnrekrtl lhnttfrqyd vrkyymspdl nflllvhdiv
      181 pvfrhsftak ykiydfsnre vfplkhssnq eelqyaawgq tgsqltyvfe nnlylipsvg
      241 dsspvqltnt gvpgvvfngv adwlyeeevl gssgalwwsp hgsrlcyatf ndsavstmpl
      301 plyegqypav lplrypkagt anpevelrva avshggaeta klvqppqell dktseyyvtm
      361 aafldeeall vnflsraqnw tlvsvcreaa sawhcskqav etasggwiel sepplvtppl
      421 kggpafflrl pvqdahrgnf rhlaafqdkr rtwlthgpyd tiaalafrpk tlhlfyrstr
      481 egrpgeshvy qvpfdkegaq pvcltchlgd qclynnawfs peaefyvlec lgpgvpwval
      541 ystadhkqle mlesnaelke lvenrampqv rnlvaplpdg ynatvrlllp pglrdeevlk
      601 ypflvnvygg pgsqqvtelf rinwghylas rkgivyglvd grgsglqgdr rlhqvyrrlg
      661 tvevedqlrv arhlknefsf issehtaiwg wsyggyvsam alanggpgnn gndtphggae
      721 ggavfqcais vapvsnwlyy dsayterymg lprdnlagye radltraaar lkgkkfllvh
      781 gtaddnvhfq hsmmlakelt rkgvlyktqv ypdekhalsh vtthlyname dfiddcfegs
      841 dhaveevglm qvkas
//
LOCUS       XP_077503731             844 aa            linear   INV 25-NOV-2025
DEFINITION  prolyl endopeptidase FAP-like isoform X8 [Amblyomma americanum].
ACCESSION   XP_077503731
VERSION     XP_077503731.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647605.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..844
                     /product="prolyl endopeptidase FAP-like isoform X8"
                     /calculated_mol_wt=93638
     Region          156..526
                     /region_name="DPPIV_N"
                     /note="Dipeptidyl peptidase IV (DPP IV) N-terminal region;
                     pfam00930"
                     /db_xref="CDD:395744"
     Region          609..829
                     /region_name="Peptidase_S9"
                     /note="Prolyl oligopeptidase family; pfam00326"
                     /db_xref="CDD:459761"
     CDS             1..844
                     /gene="LOC144114112"
                     /coded_by="XM_077647605.1:786..3320"
                     /db_xref="GeneID:144114112"
ORIGIN      
        1 mnysggtssv pgkvqggler ksttgsakrt spqdptlpeq fselvssapd srnwrgigia
       61 llviagvcal ivtavilltp gdkgprvkqa risleevlhg tfnprkfngs wvsatdfvyr
      121 dadgalvlyn aqnrekrtll hnttfrqydv rkyymspdln flllvhdivp vfrhsftaky
      181 kiydfsnrev fplkhssnqe elqyaawgqt gsqltyvfen nlylipsvgd sspvqltntg
      241 vpgvvfngva dwlyeeevlg ssgalwwsph gsrlcyatfn dsavstmplp lyegqypavl
      301 plrypkagta npevelrvaa vshggaetak lvqppqelld ktseyyvtma afldeeallv
      361 nflsraqnwt lvsvcreaas awhcskqave tasggwiels epplvtpplk ggpafflrlp
      421 vqdahrgnfr hlaafqdkrr twlthgpydt iaalafrpkt lhlfyrstre grpgeshvyq
      481 vpfdkegaqp vcltchlgdq clynnawfsp eaefyvlecl gpgvpwvaly stadhkqlem
      541 lesnaelkel venrampqvr nlvaplpdgy natvrlllpp glrdeevlky pflvnvyggp
      601 gsqqvtelfr inwghylasr kgivyglvdg rgsglqgdrr lhqvyrrlgt vevedqlrva
      661 rhlknefsfi ssehtaiwgw syggyvsama langgpgnng ndtphggaeg gavfqcaisv
      721 apvsnwlyyd sayterymgl prdnlagyer adltraaarl kgkkfllvhg taddnvhfqh
      781 smmlakeltr kgvlyktqvy pdekhalshv tthlynamed fiddcfegsd haveevglmq
      841 vkas
//
LOCUS       XP_077503732             843 aa            linear   INV 25-NOV-2025
DEFINITION  inactive dipeptidyl peptidase 10-like isoform X9 [Amblyomma
            americanum].
ACCESSION   XP_077503732
VERSION     XP_077503732.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647606.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..843
                     /product="inactive dipeptidyl peptidase 10-like isoform
                     X9"
                     /calculated_mol_wt=93581
     Region          155..525
                     /region_name="DPPIV_N"
                     /note="Dipeptidyl peptidase IV (DPP IV) N-terminal region;
                     pfam00930"
                     /db_xref="CDD:395744"
     Region          608..828
                     /region_name="Peptidase_S9"
                     /note="Prolyl oligopeptidase family; pfam00326"
                     /db_xref="CDD:459761"
     CDS             1..843
                     /gene="LOC144114112"
                     /coded_by="XM_077647606.1:785..3316"
                     /db_xref="GeneID:144114112"
ORIGIN      
        1 mnysggtssv pgkvqggler ksttgsakrt spqdptlpeq fselvssapd srnwrgigia
       61 llviagvcal ivtavilltp dkgprvkqar isleevlhgt fnprkfngsw vsatdfvyrd
      121 adgalvlyna qnrekrtllh nttfrqydvr kyymspdlnf lllvhdivpv frhsftakyk
      181 iydfsnrevf plkhssnqee lqyaawgqtg sqltyvfenn lylipsvgds spvqltntgv
      241 pgvvfngvad wlyeeevlgs sgalwwsphg srlcyatfnd savstmplpl yegqypavlp
      301 lrypkagtan pevelrvaav shggaetakl vqppqelldk tseyyvtmaa fldeeallvn
      361 flsraqnwtl vsvcreaasa whcskqavet asggwielse pplvtpplkg gpafflrlpv
      421 qdahrgnfrh laafqdkrrt wlthgpydti aalafrpktl hlfyrstreg rpgeshvyqv
      481 pfdkegaqpv cltchlgdqc lynnawfspe aefyvleclg pgvpwvalys tadhkqleml
      541 esnaelkelv enrampqvrn lvaplpdgyn atvrlllppg lrdeevlkyp flvnvyggpg
      601 sqqvtelfri nwghylasrk givyglvdgr gsglqgdrrl hqvyrrlgtv evedqlrvar
      661 hlknefsfis sehtaiwgws yggyvsamal anggpgnngn dtphggaegg avfqcaisva
      721 pvsnwlyyds ayterymglp rdnlagyera dltraaarlk gkkfllvhgt addnvhfqhs
      781 mmlakeltrk gvlyktqvyp dekhalshvt thlynamedf iddcfegsdh aveevglmqv
      841 kas
//
LOCUS       XP_077503733             843 aa            linear   INV 25-NOV-2025
DEFINITION  prolyl endopeptidase FAP-like isoform X10 [Amblyomma americanum].
ACCESSION   XP_077503733
VERSION     XP_077503733.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647607.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..843
                     /product="prolyl endopeptidase FAP-like isoform X10"
                     /calculated_mol_wt=93542
     Region          155..525
                     /region_name="DPPIV_N"
                     /note="Dipeptidyl peptidase IV (DPP IV) N-terminal region;
                     pfam00930"
                     /db_xref="CDD:395744"
     Region          608..828
                     /region_name="Peptidase_S9"
                     /note="Prolyl oligopeptidase family; pfam00326"
                     /db_xref="CDD:459761"
     CDS             1..843
                     /gene="LOC144114112"
                     /coded_by="XM_077647607.1:79..2610"
                     /db_xref="GeneID:144114112"
ORIGIN      
        1 msvasagtlq elvssapdsr nwrgigiall viagvcaliv tavilltpgp tgnalstglr
       61 kaiegladny ivrrvldqeg dkgprvkqar isleevlhgt fnprkfngsw vsatdfvyrd
      121 adgalvlyna qnrekrtllh nttfrqydvr kyymspdlnf lllvhdivpv frhsftakyk
      181 iydfsnrevf plkhssnqee lqyaawgqtg sqltyvfenn lylipsvgds spvqltntgv
      241 pgvvfngvad wlyeeevlgs sgalwwsphg srlcyatfnd savstmplpl yegqypavlp
      301 lrypkagtan pevelrvaav shggaetakl vqppqelldk tseyyvtmaa fldeeallvn
      361 flsraqnwtl vsvcreaasa whcskqavet asggwielse pplvtpplkg gpafflrlpv
      421 qdahrgnfrh laafqdkrrt wlthgpydti aalafrpktl hlfyrstreg rpgeshvyqv
      481 pfdkegaqpv cltchlgdqc lynnawfspe aefyvleclg pgvpwvalys tadhkqleml
      541 esnaelkelv enrampqvrn lvaplpdgyn atvrlllppg lrdeevlkyp flvnvyggpg
      601 sqqvtelfri nwghylasrk givyglvdgr gsglqgdrrl hqvyrrlgtv evedqlrvar
      661 hlknefsfis sehtaiwgws yggyvsamal anggpgnngn dtphggaegg avfqcaisva
      721 pvsnwlyyds ayterymglp rdnlagyera dltraaarlk gkkfllvhgt addnvhfqhs
      781 mmlakeltrk gvlyktqvyp dekhalshvt thlynamedf iddcfegsdh aveevglmqv
      841 kas
//
LOCUS       XP_077503734             833 aa            linear   INV 25-NOV-2025
DEFINITION  prolyl endopeptidase FAP-like isoform X11 [Amblyomma americanum].
ACCESSION   XP_077503734
VERSION     XP_077503734.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647608.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..833
                     /product="prolyl endopeptidase FAP-like isoform X11"
                     /calculated_mol_wt=92595
     Region          145..515
                     /region_name="DPPIV_N"
                     /note="Dipeptidyl peptidase IV (DPP IV) N-terminal region;
                     pfam00930"
                     /db_xref="CDD:395744"
     Region          598..818
                     /region_name="Peptidase_S9"
                     /note="Prolyl oligopeptidase family; pfam00326"
                     /db_xref="CDD:459761"
     CDS             1..833
                     /gene="LOC144114112"
                     /coded_by="XM_077647608.1:96..2597"
                     /db_xref="GeneID:144114112"
ORIGIN      
        1 mrleagafle dqadpgdgvg rqmdltqssc qelvssapds rnwrgigial lviagvcali
       61 vtavilltpg dkgprvkqar isleevlhgt fnprkfngsw vsatdfvyrd adgalvlyna
      121 qnrekrtllh nttfrqydvr kyymspdlnf lllvhdivpv frhsftakyk iydfsnrevf
      181 plkhssnqee lqyaawgqtg sqltyvfenn lylipsvgds spvqltntgv pgvvfngvad
      241 wlyeeevlgs sgalwwsphg srlcyatfnd savstmplpl yegqypavlp lrypkagtan
      301 pevelrvaav shggaetakl vqppqelldk tseyyvtmaa fldeeallvn flsraqnwtl
      361 vsvcreaasa whcskqavet asggwielse pplvtpplkg gpafflrlpv qdahrgnfrh
      421 laafqdkrrt wlthgpydti aalafrpktl hlfyrstreg rpgeshvyqv pfdkegaqpv
      481 cltchlgdqc lynnawfspe aefyvleclg pgvpwvalys tadhkqleml esnaelkelv
      541 enrampqvrn lvaplpdgyn atvrlllppg lrdeevlkyp flvnvyggpg sqqvtelfri
      601 nwghylasrk givyglvdgr gsglqgdrrl hqvyrrlgtv evedqlrvar hlknefsfis
      661 sehtaiwgws yggyvsamal anggpgnngn dtphggaegg avfqcaisva pvsnwlyyds
      721 ayterymglp rdnlagyera dltraaarlk gkkfllvhgt addnvhfqhs mmlakeltrk
      781 gvlyktqvyp dekhalshvt thlynamedf iddcfegsdh aveevglmqv kas
//
LOCUS       XP_077503736             832 aa            linear   INV 25-NOV-2025
DEFINITION  inactive dipeptidyl peptidase 10-like isoform X12 [Amblyomma
            americanum].
ACCESSION   XP_077503736
VERSION     XP_077503736.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647610.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..832
                     /product="inactive dipeptidyl peptidase 10-like isoform
                     X12"
                     /calculated_mol_wt=92538
     Region          144..514
                     /region_name="DPPIV_N"
                     /note="Dipeptidyl peptidase IV (DPP IV) N-terminal region;
                     pfam00930"
                     /db_xref="CDD:395744"
     Region          597..817
                     /region_name="Peptidase_S9"
                     /note="Prolyl oligopeptidase family; pfam00326"
                     /db_xref="CDD:459761"
     CDS             1..832
                     /gene="LOC144114112"
                     /coded_by="XM_077647610.1:95..2593"
                     /db_xref="GeneID:144114112"
ORIGIN      
        1 mrleagafle dqadpgdgvg rqmdltqssc qelvssapds rnwrgigial lviagvcali
       61 vtavilltpd kgprvkqari sleevlhgtf nprkfngswv satdfvyrda dgalvlynaq
      121 nrekrtllhn ttfrqydvrk yymspdlnfl llvhdivpvf rhsftakyki ydfsnrevfp
      181 lkhssnqeel qyaawgqtgs qltyvfennl ylipsvgdss pvqltntgvp gvvfngvadw
      241 lyeeevlgss galwwsphgs rlcyatfnds avstmplply egqypavlpl rypkagtanp
      301 evelrvaavs hggaetaklv qppqelldkt seyyvtmaaf ldeeallvnf lsraqnwtlv
      361 svcreaasaw hcskqaveta sggwielsep plvtpplkgg pafflrlpvq dahrgnfrhl
      421 aafqdkrrtw lthgpydtia alafrpktlh lfyrstregr pgeshvyqvp fdkegaqpvc
      481 ltchlgdqcl ynnawfspea efyvleclgp gvpwvalyst adhkqlemle snaelkelve
      541 nrampqvrnl vaplpdgyna tvrlllppgl rdeevlkypf lvnvyggpgs qqvtelfrin
      601 wghylasrkg ivyglvdgrg sglqgdrrlh qvyrrlgtve vedqlrvarh lknefsfiss
      661 ehtaiwgwsy ggyvsamala nggpgnngnd tphggaegga vfqcaisvap vsnwlyydsa
      721 yterymglpr dnlagyerad ltraaarlkg kkfllvhgta ddnvhfqhsm mlakeltrkg
      781 vlyktqvypd ekhalshvtt hlynamedfi ddcfegsdha veevglmqvk as
//
LOCUS       XP_077503737             824 aa            linear   INV 25-NOV-2025
DEFINITION  prolyl endopeptidase FAP-like isoform X13 [Amblyomma americanum].
ACCESSION   XP_077503737
VERSION     XP_077503737.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647611.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..824
                     /product="prolyl endopeptidase FAP-like isoform X13"
                     /calculated_mol_wt=91534
     Region          136..506
                     /region_name="DPPIV_N"
                     /note="Dipeptidyl peptidase IV (DPP IV) N-terminal region;
                     pfam00930"
                     /db_xref="CDD:395744"
     Region          589..809
                     /region_name="Peptidase_S9"
                     /note="Prolyl oligopeptidase family; pfam00326"
                     /db_xref="CDD:459761"
     CDS             1..824
                     /gene="LOC144114112"
                     /coded_by="XM_077647611.1:58..2532"
                     /db_xref="GeneID:144114112"
ORIGIN      
        1 mtssaepkkk ptaapvpqdp naelvssapd srnwrgigia llviagvcal ivtavilltp
       61 gdkgprvkqa risleevlhg tfnprkfngs wvsatdfvyr dadgalvlyn aqnrekrtll
      121 hnttfrqydv rkyymspdln flllvhdivp vfrhsftaky kiydfsnrev fplkhssnqe
      181 elqyaawgqt gsqltyvfen nlylipsvgd sspvqltntg vpgvvfngva dwlyeeevlg
      241 ssgalwwsph gsrlcyatfn dsavstmplp lyegqypavl plrypkagta npevelrvaa
      301 vshggaetak lvqppqelld ktseyyvtma afldeeallv nflsraqnwt lvsvcreaas
      361 awhcskqave tasggwiels epplvtpplk ggpafflrlp vqdahrgnfr hlaafqdkrr
      421 twlthgpydt iaalafrpkt lhlfyrstre grpgeshvyq vpfdkegaqp vcltchlgdq
      481 clynnawfsp eaefyvlecl gpgvpwvaly stadhkqlem lesnaelkel venrampqvr
      541 nlvaplpdgy natvrlllpp glrdeevlky pflvnvyggp gsqqvtelfr inwghylasr
      601 kgivyglvdg rgsglqgdrr lhqvyrrlgt vevedqlrva rhlknefsfi ssehtaiwgw
      661 syggyvsama langgpgnng ndtphggaeg gavfqcaisv apvsnwlyyd sayterymgl
      721 prdnlagyer adltraaarl kgkkfllvhg taddnvhfqh smmlakeltr kgvlyktqvy
      781 pdekhalshv tthlynamed fiddcfegsd haveevglmq vkas
//
LOCUS       XP_077503738             823 aa            linear   INV 25-NOV-2025
DEFINITION  inactive dipeptidyl peptidase 10-like isoform X14 [Amblyomma
            americanum].
ACCESSION   XP_077503738
VERSION     XP_077503738.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647612.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..823
                     /product="inactive dipeptidyl peptidase 10-like isoform
                     X14"
                     /calculated_mol_wt=91477
     Region          135..505
                     /region_name="DPPIV_N"
                     /note="Dipeptidyl peptidase IV (DPP IV) N-terminal region;
                     pfam00930"
                     /db_xref="CDD:395744"
     Region          588..808
                     /region_name="Peptidase_S9"
                     /note="Prolyl oligopeptidase family; pfam00326"
                     /db_xref="CDD:459761"
     CDS             1..823
                     /gene="LOC144114112"
                     /coded_by="XM_077647612.1:58..2529"
                     /db_xref="GeneID:144114112"
ORIGIN      
        1 mtssaepkkk ptaapvpqdp naelvssapd srnwrgigia llviagvcal ivtavilltp
       61 dkgprvkqar isleevlhgt fnprkfngsw vsatdfvyrd adgalvlyna qnrekrtllh
      121 nttfrqydvr kyymspdlnf lllvhdivpv frhsftakyk iydfsnrevf plkhssnqee
      181 lqyaawgqtg sqltyvfenn lylipsvgds spvqltntgv pgvvfngvad wlyeeevlgs
      241 sgalwwsphg srlcyatfnd savstmplpl yegqypavlp lrypkagtan pevelrvaav
      301 shggaetakl vqppqelldk tseyyvtmaa fldeeallvn flsraqnwtl vsvcreaasa
      361 whcskqavet asggwielse pplvtpplkg gpafflrlpv qdahrgnfrh laafqdkrrt
      421 wlthgpydti aalafrpktl hlfyrstreg rpgeshvyqv pfdkegaqpv cltchlgdqc
      481 lynnawfspe aefyvleclg pgvpwvalys tadhkqleml esnaelkelv enrampqvrn
      541 lvaplpdgyn atvrlllppg lrdeevlkyp flvnvyggpg sqqvtelfri nwghylasrk
      601 givyglvdgr gsglqgdrrl hqvyrrlgtv evedqlrvar hlknefsfis sehtaiwgws
      661 yggyvsamal anggpgnngn dtphggaegg avfqcaisva pvsnwlyyds ayterymglp
      721 rdnlagyera dltraaarlk gkkfllvhgt addnvhfqhs mmlakeltrk gvlyktqvyp
      781 dekhalshvt thlynamedf iddcfegsdh aveevglmqv kas
//
LOCUS       XP_077503739             818 aa            linear   INV 25-NOV-2025
DEFINITION  prolyl endopeptidase FAP-like isoform X15 [Amblyomma americanum].
ACCESSION   XP_077503739
VERSION     XP_077503739.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647613.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..818
                     /product="prolyl endopeptidase FAP-like isoform X15"
                     /calculated_mol_wt=91121
     Region          130..500
                     /region_name="DPPIV_N"
                     /note="Dipeptidyl peptidase IV (DPP IV) N-terminal region;
                     pfam00930"
                     /db_xref="CDD:395744"
     Region          583..803
                     /region_name="Peptidase_S9"
                     /note="Prolyl oligopeptidase family; pfam00326"
                     /db_xref="CDD:459761"
     CDS             1..818
                     /gene="LOC144114112"
                     /coded_by="XM_077647613.1:16..2472"
                     /db_xref="GeneID:144114112"
ORIGIN      
        1 mnlcrknaer tsqtslelvs sapdsrnwrg igiallviag vcalivtavi lltpgdkgpr
       61 vkqarislee vlhgtfnprk fngswvsatd fvyrdadgal vlynaqnrek rtllhnttfr
      121 qydvrkyyms pdlnflllvh divpvfrhsf takykiydfs nrevfplkhs snqeelqyaa
      181 wgqtgsqlty vfennlylip svgdsspvql tntgvpgvvf ngvadwlyee evlgssgalw
      241 wsphgsrlcy atfndsavst mplplyegqy pavlplrypk agtanpevel rvaavshgga
      301 etaklvqppq elldktseyy vtmaafldee allvnflsra qnwtlvsvcr eaasawhcsk
      361 qavetasggw ielsepplvt pplkggpaff lrlpvqdahr gnfrhlaafq dkrrtwlthg
      421 pydtiaalaf rpktlhlfyr stregrpges hvyqvpfdke gaqpvcltch lgdqclynna
      481 wfspeaefyv leclgpgvpw valystadhk qlemlesnae lkelvenram pqvrnlvapl
      541 pdgynatvrl llppglrdee vlkypflvnv yggpgsqqvt elfrinwghy lasrkgivyg
      601 lvdgrgsglq gdrrlhqvyr rlgtvevedq lrvarhlkne fsfissehta iwgwsyggyv
      661 samalanggp gnngndtphg gaeggavfqc aisvapvsnw lyydsayter ymglprdnla
      721 gyeradltra aarlkgkkfl lvhgtaddnv hfqhsmmlak eltrkgvlyk tqvypdekha
      781 lshvtthlyn amedfiddcf egsdhaveev glmqvkas
//
LOCUS       XP_077503740             690 aa            linear   INV 25-NOV-2025
DEFINITION  prolyl endopeptidase FAP-like isoform X16 [Amblyomma americanum].
ACCESSION   XP_077503740
VERSION     XP_077503740.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647614.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..690
                     /product="prolyl endopeptidase FAP-like isoform X16"
                     /calculated_mol_wt=76740
     Region          2..372
                     /region_name="DPPIV_N"
                     /note="Dipeptidyl peptidase IV (DPP IV) N-terminal region;
                     pfam00930"
                     /db_xref="CDD:395744"
     Region          455..675
                     /region_name="Peptidase_S9"
                     /note="Prolyl oligopeptidase family; pfam00326"
                     /db_xref="CDD:459761"
     CDS             1..690
                     /gene="LOC144114112"
                     /coded_by="XM_077647614.1:559..2631"
                     /db_xref="GeneID:144114112"
ORIGIN      
        1 mspdlnflll vhdivpvfrh sftakykiyd fsnrevfplk hssnqeelqy aawgqtgsql
       61 tyvfennlyl ipsvgdsspv qltntgvpgv vfngvadwly eeevlgssga lwwsphgsrl
      121 cyatfndsav stmplplyeg qypavlplry pkagtanpev elrvaavshg gaetaklvqp
      181 pqelldktse yyvtmaafld eeallvnfls raqnwtlvsv creaasawhc skqavetasg
      241 gwielseppl vtpplkggpa fflrlpvqda hrgnfrhlaa fqdkrrtwlt hgpydtiaal
      301 afrpktlhlf yrstregrpg eshvyqvpfd kegaqpvclt chlgdqclyn nawfspeaef
      361 yvleclgpgv pwvalystad hkqlemlesn aelkelvenr ampqvrnlva plpdgynatv
      421 rlllppglrd eevlkypflv nvyggpgsqq vtelfrinwg hylasrkgiv yglvdgrgsg
      481 lqgdrrlhqv yrrlgtveve dqlrvarhlk nefsfisseh taiwgwsygg yvsamalang
      541 gpgnngndtp hggaeggavf qcaisvapvs nwlyydsayt erymglprdn lagyeradlt
      601 raaarlkgkk fllvhgtadd nvhfqhsmml akeltrkgvl yktqvypdek halshvtthl
      661 ynamedfidd cfegsdhave evglmqvkas
//
LOCUS       XP_077503741             690 aa            linear   INV 25-NOV-2025
DEFINITION  prolyl endopeptidase FAP-like isoform X16 [Amblyomma americanum].
ACCESSION   XP_077503741
VERSION     XP_077503741.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647615.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..690
                     /product="prolyl endopeptidase FAP-like isoform X16"
                     /calculated_mol_wt=76740
     Region          2..372
                     /region_name="DPPIV_N"
                     /note="Dipeptidyl peptidase IV (DPP IV) N-terminal region;
                     pfam00930"
                     /db_xref="CDD:395744"
     Region          455..675
                     /region_name="Peptidase_S9"
                     /note="Prolyl oligopeptidase family; pfam00326"
                     /db_xref="CDD:459761"
     CDS             1..690
                     /gene="LOC144114112"
                     /coded_by="XM_077647615.1:463..2535"
                     /db_xref="GeneID:144114112"
ORIGIN      
        1 mspdlnflll vhdivpvfrh sftakykiyd fsnrevfplk hssnqeelqy aawgqtgsql
       61 tyvfennlyl ipsvgdsspv qltntgvpgv vfngvadwly eeevlgssga lwwsphgsrl
      121 cyatfndsav stmplplyeg qypavlplry pkagtanpev elrvaavshg gaetaklvqp
      181 pqelldktse yyvtmaafld eeallvnfls raqnwtlvsv creaasawhc skqavetasg
      241 gwielseppl vtpplkggpa fflrlpvqda hrgnfrhlaa fqdkrrtwlt hgpydtiaal
      301 afrpktlhlf yrstregrpg eshvyqvpfd kegaqpvclt chlgdqclyn nawfspeaef
      361 yvleclgpgv pwvalystad hkqlemlesn aelkelvenr ampqvrnlva plpdgynatv
      421 rlllppglrd eevlkypflv nvyggpgsqq vtelfrinwg hylasrkgiv yglvdgrgsg
      481 lqgdrrlhqv yrrlgtveve dqlrvarhlk nefsfisseh taiwgwsygg yvsamalang
      541 gpgnngndtp hggaeggavf qcaisvapvs nwlyydsayt erymglprdn lagyeradlt
      601 raaarlkgkk fllvhgtadd nvhfqhsmml akeltrkgvl yktqvypdek halshvtthl
      661 ynamedfidd cfegsdhave evglmqvkas
//
LOCUS       XP_077503742             266 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114114 [Amblyomma americanum].
ACCESSION   XP_077503742
VERSION     XP_077503742.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647616.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..266
                     /product="uncharacterized protein LOC144114114"
                     /calculated_mol_wt=28164
     Region          99..213
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     CDS             1..266
                     /gene="LOC144114114"
                     /coded_by="XM_077647616.1:268..1068"
                     /db_xref="GeneID:144114114"
ORIGIN      
        1 mtcalslhrr qqrrpedqlc dieadratpn cpqaassipq vqaarpgapa grsirpgelr
       61 lepanvdnla ntavmvrgrc drfseavdgr halndsccsl aaaatipalg lhsqqhaafl
      121 gssttaelmg irlgldlllt lgpppprsal lcdsraalsr lqsngrgtpl vreirsrier
      181 laqrgcavra qwvpghcgia gneeaddlat aahqlpasdl plaledvraa ihdhlrkqct
      241 pthasrevsa stlspavahl hghnvq
//
LOCUS       XP_077503743             240 aa            linear   INV 25-NOV-2025
DEFINITION  6-phosphogluconolactonase [Amblyomma americanum].
ACCESSION   XP_077503743
VERSION     XP_077503743.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647617.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..240
                     /product="6-phosphogluconolactonase"
                     /calculated_mol_wt=26408
     Region          21..230
                     /region_name="6PGL"
                     /note="6-Phosphogluconolactonase (6PGL) subfamily; 6PGL
                     catalyzes the second step of the oxidative phase of the
                     pentose phosphate pathway, the hydrolyzation of
                     6-phosphoglucono-1,5-lactone (delta form) to
                     6-phosphogluconate. 6PGL is thought to guard against
                     the...; cd01400"
                     /db_xref="CDD:238694"
     Site            order(45..46,72,143,174,197)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238694"
     CDS             1..240
                     /gene="Pgls"
                     /coded_by="XM_077647617.1:140..862"
                     /db_xref="GeneID:144114115"
ORIGIN      
        1 mtlklivtdd karllsrlkl liqqvadnfv ssgneyikvg vsggsmvgml aslllevktd
       61 wqkwrfffcd erlvpfdnse stfgcyraql lpklnlkeeq fvivnpslsp sdaardycek
      121 lakyfnavpe fdllllgmgp dghtcslfpg hklleekskw vaeitdspkp ppcrvtltlp
      181 vinsaklvvf pisgvekagt vlkvlhpkdk eqplpaslvq ptkgdviwll dsaaasllpk
//
LOCUS       XP_077503744            1044 aa            linear   INV 25-NOV-2025
DEFINITION  serine/threonine-protein kinase N isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503744
VERSION     XP_077503744.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647618.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1044
                     /product="serine/threonine-protein kinase N isoform X1"
                     /calculated_mol_wt=116674
     Region          47..112
                     /region_name="HR1_PKN_1"
                     /note="First Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11622"
                     /db_xref="CDD:212012"
     Site            order(55,58,61..62,65..66,68..69,72..73,75..76,91..92,95,
                     98..99,102)
                     /site_type="other"
                     /note="Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212012"
     Site            order(63..64,66..67,70..71,73..74,77..78,81,86..87,89)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212012"
     Region          146..215
                     /region_name="HR1_PKN_2"
                     /note="Second Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11623"
                     /db_xref="CDD:212013"
     Site            order(153,156,159..160,163..164,166..167,170..171,
                     173..174,194..195,198,201..202,205)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212013"
     Site            order(161..162,164..165,168..169,171..172,175..176,
                     189..190,192)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212013"
     Region          241..314
                     /region_name="HR1_PKN_3"
                     /note="Third Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11625"
                     /db_xref="CDD:212015"
     Site            order(251,254,257..258,261..262,264..265,268..269,
                     271..272,293..294,297,300..301,304)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212015"
     Site            order(259..260,262..263,266..267,269..270,273..274,
                     288..289,291)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212015"
     Region          438..520
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          717..1042
                     /region_name="STKc_PKN"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Protein Kinase N; cd05589"
                     /db_xref="CDD:270741"
     Site            order(723..727,731,744,746,781,797..798,800,804,806,842,
                     844,846..847,849,859..860,863,877..882,909,915,918)
                     /site_type="active"
                     /db_xref="CDD:270741"
     Site            order(723..727,731,744,746,781,798..800,804,842,844,
                     846..847,849,859..860)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270741"
     Site            order(727,804,806,842,844,846,863,877..882,909,915,918)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270741"
     Site            859..882
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270741"
     Site            1018
                     /site_type="other"
                     /note="turn motif phosphorylation site [posttranslational
                     modification]"
                     /db_xref="CDD:270741"
     Site            1034..1039
                     /site_type="other"
                     /note="hydrophobic motif (HM)"
                     /db_xref="CDD:270741"
     CDS             1..1044
                     /gene="Pkn"
                     /coded_by="XM_077647618.1:211..3345"
                     /db_xref="GeneID:144114116"
ORIGIN      
        1 msysyyrvwa pdmsrvasqe iagkyglgga argsgdgsss snepslqrql eelkrklked
       61 mrkelkikeg aenlrkattd rkslahvnsi vkkansrlnd lqrqlsdlta dilvtqghps
      121 pqgmvrraqp rstdsfygdk gdmplsttdq rlqslekqln ielkvkqgae nmlqmyssgp
      181 skdrkllaea qqmqadskak idyirmmmar lrqnqdshsk etgegcsnnh ldsttggkip
      241 evmspvelqi edlrhrlkve cavvegaknv lklcqaagkv tdrkvfqeaq nslvessekl
      301 dlirrslelr raelspgsak wqllkreles sqlvspysps ivphgpraeq pgslasplsp
      361 laasptisls kpasvtgqlq vrlmgcqdll edvpgrlprs qvagvgadlr sslvratgrg
      421 fsgrnssrsy sikeetssei mavlkldnvh vgqttykpcs qqawdqrftv aldrsrelei
      481 qiywkdwrgm cavkflrlee fidderhgma lhlepkgllf aeirfqnpmi srkprlqrqr
      541 klfihkgknf lrptqmninv atwgrlmkra mppgcsdqvt tvsppdallt tapvstspel
      601 dslvvtklsf edtnepdass plsshqtsle tdvqsalgef dflqqetsit smaveekaqq
      661 tsnmvvgghs pspepivela pdeedevnkl geppqvtpvv vvpdvesgqg sltldhfefi
      721 svlgrghfgk vilskhktsg qyfaikalkk gdivardele slmaekrife vansvqhpfl
      781 vnlfacfqtt nhvcfvmeya cggdlmmhih tdifpepraa fyaacvvlgl qflhenriiy
      841 rdlkldnlll daegyvklad fglckegmgy gdrtgtfcgt peflapevlt etsytravdw
      901 wglgvlvfem lvgespfpgd deeevfdsiv hdevryprfl sieavaimrr llrknperrl
      961 gtsekdaedv kkqaffrnin wehllvkklk ppfvptvksp edvsnfddef tceqpvltap
     1021 kdprpltqee qahfkdftyt snwc
//
LOCUS       XP_077503745            1040 aa            linear   INV 25-NOV-2025
DEFINITION  serine/threonine-protein kinase N isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503745
VERSION     XP_077503745.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647619.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1040
                     /product="serine/threonine-protein kinase N isoform X2"
                     /calculated_mol_wt=116132
     Region          47..112
                     /region_name="HR1_PKN_1"
                     /note="First Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11622"
                     /db_xref="CDD:212012"
     Site            order(55,58,61..62,65..66,68..69,72..73,75..76,91..92,95,
                     98..99,102)
                     /site_type="other"
                     /note="Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212012"
     Site            order(63..64,66..67,70..71,73..74,77..78,81,86..87,89)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212012"
     Region          142..211
                     /region_name="HR1_PKN_2"
                     /note="Second Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11623"
                     /db_xref="CDD:212013"
     Site            order(149,152,155..156,159..160,162..163,166..167,
                     169..170,190..191,194,197..198,201)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212013"
     Site            order(157..158,160..161,164..165,167..168,171..172,
                     185..186,188)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212013"
     Region          237..310
                     /region_name="HR1_PKN_3"
                     /note="Third Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11625"
                     /db_xref="CDD:212015"
     Site            order(247,250,253..254,257..258,260..261,264..265,
                     267..268,289..290,293,296..297,300)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212015"
     Site            order(255..256,258..259,262..263,265..266,269..270,
                     284..285,287)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212015"
     Region          434..516
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          713..1038
                     /region_name="STKc_PKN"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Protein Kinase N; cd05589"
                     /db_xref="CDD:270741"
     Site            order(719..723,727,740,742,777,793..794,796,800,802,838,
                     840,842..843,845,855..856,859,873..878,905,911,914)
                     /site_type="active"
                     /db_xref="CDD:270741"
     Site            order(719..723,727,740,742,777,794..796,800,838,840,
                     842..843,845,855..856)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270741"
     Site            order(723,800,802,838,840,842,859,873..878,905,911,914)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270741"
     Site            855..878
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270741"
     Site            1014
                     /site_type="other"
                     /note="turn motif phosphorylation site [posttranslational
                     modification]"
                     /db_xref="CDD:270741"
     Site            1030..1035
                     /site_type="other"
                     /note="hydrophobic motif (HM)"
                     /db_xref="CDD:270741"
     CDS             1..1040
                     /gene="Pkn"
                     /coded_by="XM_077647619.1:211..3333"
                     /db_xref="GeneID:144114116"
ORIGIN      
        1 msysyyrvwa pdmsrvasqe iagkyglgga argsgdgsss snepslqrql eelkrklked
       61 mrkelkikeg aenlrkattd rkslahvnsi vkkansrlnd lqrqlsdlta dilvtqghps
      121 pqgaqprstd sfygdkgdmp lsttdqrlqs lekqlnielk vkqgaenmlq myssgpskdr
      181 kllaeaqqmq adskakidyi rmmmarlrqn qdshsketge gcsnnhldst tggkipevms
      241 pvelqiedlr hrlkvecavv egaknvlklc qaagkvtdrk vfqeaqnslv essekldlir
      301 rslelrrael spgsakwqll krelessqlv spyspsivph gpraeqpgsl asplsplaas
      361 ptislskpas vtgqlqvrlm gcqdlledvp grlprsqvag vgadlrsslv ratgrgfsgr
      421 nssrsysike etsseimavl kldnvhvgqt tykpcsqqaw dqrftvaldr sreleiqiyw
      481 kdwrgmcavk flrleefidd erhgmalhle pkgllfaeir fqnpmisrkp rlqrqrklfi
      541 hkgknflrpt qmninvatwg rlmkramppg csdqvttvsp pdallttapv stspeldslv
      601 vtklsfedtn epdassplss hqtsletdvq salgefdflq qetsitsmav eekaqqtsnm
      661 vvgghspspe pivelapdee devnklgepp qvtpvvvvpd vesgqgsltl dhfefisvlg
      721 rghfgkvils khktsgqyfa ikalkkgdiv ardeleslma ekrifevans vqhpflvnlf
      781 acfqttnhvc fvmeyacggd lmmhihtdif pepraafyaa cvvlglqflh enriiyrdlk
      841 ldnllldaeg yvkladfglc kegmgygdrt gtfcgtpefl apevltetsy travdwwglg
      901 vlvfemlvge spfpgddeee vfdsivhdev ryprflsiea vaimrrllrk nperrlgtse
      961 kdaedvkkqa ffrninwehl lvkklkppfv ptvkspedvs nfddeftceq pvltapkdpr
     1021 pltqeeqahf kdftytsnwc
//
LOCUS       XP_077503746            1032 aa            linear   INV 25-NOV-2025
DEFINITION  serine/threonine-protein kinase N isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503746
VERSION     XP_077503746.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647620.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1032
                     /product="serine/threonine-protein kinase N isoform X3"
                     /calculated_mol_wt=115155
     Region          35..100
                     /region_name="HR1_PKN_1"
                     /note="First Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11622"
                     /db_xref="CDD:212012"
     Site            order(43,46,49..50,53..54,56..57,60..61,63..64,79..80,83,
                     86..87,90)
                     /site_type="other"
                     /note="Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212012"
     Site            order(51..52,54..55,58..59,61..62,65..66,69,74..75,77)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212012"
     Region          134..203
                     /region_name="HR1_PKN_2"
                     /note="Second Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11623"
                     /db_xref="CDD:212013"
     Site            order(141,144,147..148,151..152,154..155,158..159,
                     161..162,182..183,186,189..190,193)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212013"
     Site            order(149..150,152..153,156..157,159..160,163..164,
                     177..178,180)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212013"
     Region          229..302
                     /region_name="HR1_PKN_3"
                     /note="Third Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11625"
                     /db_xref="CDD:212015"
     Site            order(239,242,245..246,249..250,252..253,256..257,
                     259..260,281..282,285,288..289,292)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212015"
     Site            order(247..248,250..251,254..255,257..258,261..262,
                     276..277,279)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212015"
     Region          426..508
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          705..1030
                     /region_name="STKc_PKN"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Protein Kinase N; cd05589"
                     /db_xref="CDD:270741"
     Site            order(711..715,719,732,734,769,785..786,788,792,794,830,
                     832,834..835,837,847..848,851,865..870,897,903,906)
                     /site_type="active"
                     /db_xref="CDD:270741"
     Site            order(711..715,719,732,734,769,786..788,792,830,832,
                     834..835,837,847..848)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270741"
     Site            order(715,792,794,830,832,834,851,865..870,897,903,906)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270741"
     Site            847..870
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270741"
     Site            1006
                     /site_type="other"
                     /note="turn motif phosphorylation site [posttranslational
                     modification]"
                     /db_xref="CDD:270741"
     Site            1022..1027
                     /site_type="other"
                     /note="hydrophobic motif (HM)"
                     /db_xref="CDD:270741"
     CDS             1..1032
                     /gene="Pkn"
                     /coded_by="XM_077647620.1:219..3317"
                     /db_xref="GeneID:144114116"
ORIGIN      
        1 msrvasqeia gkyglggaar gsgdgssssn epslqrqlee lkrklkedmr kelkikegae
       61 nlrkattdrk slahvnsivk kansrlndlq rqlsdltadi lvtqghpspq gmvrraqprs
      121 tdsfygdkgd mplsttdqrl qslekqlnie lkvkqgaenm lqmyssgpsk drkllaeaqq
      181 mqadskakid yirmmmarlr qnqdshsket gegcsnnhld sttggkipev mspvelqied
      241 lrhrlkveca vvegaknvlk lcqaagkvtd rkvfqeaqns lvessekldl irrslelrra
      301 elspgsakwq llkrelessq lvspyspsiv phgpraeqpg slasplspla asptislskp
      361 asvtgqlqvr lmgcqdlled vpgrlprsqv agvgadlrss lvratgrgfs grnssrsysi
      421 keetsseima vlkldnvhvg qttykpcsqq awdqrftval drsreleiqi ywkdwrgmca
      481 vkflrleefi dderhgmalh lepkgllfae irfqnpmisr kprlqrqrkl fihkgknflr
      541 ptqmninvat wgrlmkramp pgcsdqvttv sppdalltta pvstspelds lvvtklsfed
      601 tnepdasspl sshqtsletd vqsalgefdf lqqetsitsm aveekaqqts nmvvgghsps
      661 pepivelapd eedevnklge ppqvtpvvvv pdvesgqgsl tldhfefisv lgrghfgkvi
      721 lskhktsgqy faikalkkgd ivardelesl maekrifeva nsvqhpflvn lfacfqttnh
      781 vcfvmeyacg gdlmmhihtd ifpepraafy aacvvlglqf lhenriiyrd lkldnlllda
      841 egyvkladfg lckegmgygd rtgtfcgtpe flapevltet sytravdwwg lgvlvfemlv
      901 gespfpgdde eevfdsivhd evryprflsi eavaimrrll rknperrlgt sekdaedvkk
      961 qaffrninwe hllvkklkpp fvptvksped vsnfddeftc eqpvltapkd prpltqeeqa
     1021 hfkdftytsn wc
//
LOCUS       XP_077503747            1032 aa            linear   INV 25-NOV-2025
DEFINITION  serine/threonine-protein kinase N isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503747
VERSION     XP_077503747.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647621.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1032
                     /product="serine/threonine-protein kinase N isoform X3"
                     /calculated_mol_wt=115155
     Region          35..100
                     /region_name="HR1_PKN_1"
                     /note="First Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11622"
                     /db_xref="CDD:212012"
     Site            order(43,46,49..50,53..54,56..57,60..61,63..64,79..80,83,
                     86..87,90)
                     /site_type="other"
                     /note="Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212012"
     Site            order(51..52,54..55,58..59,61..62,65..66,69,74..75,77)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212012"
     Region          134..203
                     /region_name="HR1_PKN_2"
                     /note="Second Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11623"
                     /db_xref="CDD:212013"
     Site            order(141,144,147..148,151..152,154..155,158..159,
                     161..162,182..183,186,189..190,193)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212013"
     Site            order(149..150,152..153,156..157,159..160,163..164,
                     177..178,180)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212013"
     Region          229..302
                     /region_name="HR1_PKN_3"
                     /note="Third Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11625"
                     /db_xref="CDD:212015"
     Site            order(239,242,245..246,249..250,252..253,256..257,
                     259..260,281..282,285,288..289,292)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212015"
     Site            order(247..248,250..251,254..255,257..258,261..262,
                     276..277,279)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212015"
     Region          426..508
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          705..1030
                     /region_name="STKc_PKN"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Protein Kinase N; cd05589"
                     /db_xref="CDD:270741"
     Site            order(711..715,719,732,734,769,785..786,788,792,794,830,
                     832,834..835,837,847..848,851,865..870,897,903,906)
                     /site_type="active"
                     /db_xref="CDD:270741"
     Site            order(711..715,719,732,734,769,786..788,792,830,832,
                     834..835,837,847..848)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270741"
     Site            order(715,792,794,830,832,834,851,865..870,897,903,906)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270741"
     Site            847..870
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270741"
     Site            1006
                     /site_type="other"
                     /note="turn motif phosphorylation site [posttranslational
                     modification]"
                     /db_xref="CDD:270741"
     Site            1022..1027
                     /site_type="other"
                     /note="hydrophobic motif (HM)"
                     /db_xref="CDD:270741"
     CDS             1..1032
                     /gene="Pkn"
                     /coded_by="XM_077647621.1:151..3249"
                     /db_xref="GeneID:144114116"
ORIGIN      
        1 msrvasqeia gkyglggaar gsgdgssssn epslqrqlee lkrklkedmr kelkikegae
       61 nlrkattdrk slahvnsivk kansrlndlq rqlsdltadi lvtqghpspq gmvrraqprs
      121 tdsfygdkgd mplsttdqrl qslekqlnie lkvkqgaenm lqmyssgpsk drkllaeaqq
      181 mqadskakid yirmmmarlr qnqdshsket gegcsnnhld sttggkipev mspvelqied
      241 lrhrlkveca vvegaknvlk lcqaagkvtd rkvfqeaqns lvessekldl irrslelrra
      301 elspgsakwq llkrelessq lvspyspsiv phgpraeqpg slasplspla asptislskp
      361 asvtgqlqvr lmgcqdlled vpgrlprsqv agvgadlrss lvratgrgfs grnssrsysi
      421 keetsseima vlkldnvhvg qttykpcsqq awdqrftval drsreleiqi ywkdwrgmca
      481 vkflrleefi dderhgmalh lepkgllfae irfqnpmisr kprlqrqrkl fihkgknflr
      541 ptqmninvat wgrlmkramp pgcsdqvttv sppdalltta pvstspelds lvvtklsfed
      601 tnepdasspl sshqtsletd vqsalgefdf lqqetsitsm aveekaqqts nmvvgghsps
      661 pepivelapd eedevnklge ppqvtpvvvv pdvesgqgsl tldhfefisv lgrghfgkvi
      721 lskhktsgqy faikalkkgd ivardelesl maekrifeva nsvqhpflvn lfacfqttnh
      781 vcfvmeyacg gdlmmhihtd ifpepraafy aacvvlglqf lhenriiyrd lkldnlllda
      841 egyvkladfg lckegmgygd rtgtfcgtpe flapevltet sytravdwwg lgvlvfemlv
      901 gespfpgdde eevfdsivhd evryprflsi eavaimrrll rknperrlgt sekdaedvkk
      961 qaffrninwe hllvkklkpp fvptvksped vsnfddeftc eqpvltapkd prpltqeeqa
     1021 hfkdftytsn wc
//
LOCUS       XP_077503748            1028 aa            linear   INV 25-NOV-2025
DEFINITION  serine/threonine-protein kinase N isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503748
VERSION     XP_077503748.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647622.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1028
                     /product="serine/threonine-protein kinase N isoform X4"
                     /calculated_mol_wt=114612
     Region          35..100
                     /region_name="HR1_PKN_1"
                     /note="First Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11622"
                     /db_xref="CDD:212012"
     Site            order(43,46,49..50,53..54,56..57,60..61,63..64,79..80,83,
                     86..87,90)
                     /site_type="other"
                     /note="Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212012"
     Site            order(51..52,54..55,58..59,61..62,65..66,69,74..75,77)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212012"
     Region          130..199
                     /region_name="HR1_PKN_2"
                     /note="Second Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11623"
                     /db_xref="CDD:212013"
     Site            order(137,140,143..144,147..148,150..151,154..155,
                     157..158,178..179,182,185..186,189)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212013"
     Site            order(145..146,148..149,152..153,155..156,159..160,
                     173..174,176)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212013"
     Region          225..298
                     /region_name="HR1_PKN_3"
                     /note="Third Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11625"
                     /db_xref="CDD:212015"
     Site            order(235,238,241..242,245..246,248..249,252..253,
                     255..256,277..278,281,284..285,288)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212015"
     Site            order(243..244,246..247,250..251,253..254,257..258,
                     272..273,275)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212015"
     Region          422..504
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          701..1026
                     /region_name="STKc_PKN"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Protein Kinase N; cd05589"
                     /db_xref="CDD:270741"
     Site            order(707..711,715,728,730,765,781..782,784,788,790,826,
                     828,830..831,833,843..844,847,861..866,893,899,902)
                     /site_type="active"
                     /db_xref="CDD:270741"
     Site            order(707..711,715,728,730,765,782..784,788,826,828,
                     830..831,833,843..844)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270741"
     Site            order(711,788,790,826,828,830,847,861..866,893,899,902)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270741"
     Site            843..866
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270741"
     Site            1002
                     /site_type="other"
                     /note="turn motif phosphorylation site [posttranslational
                     modification]"
                     /db_xref="CDD:270741"
     Site            1018..1023
                     /site_type="other"
                     /note="hydrophobic motif (HM)"
                     /db_xref="CDD:270741"
     CDS             1..1028
                     /gene="Pkn"
                     /coded_by="XM_077647622.1:251..3337"
                     /db_xref="GeneID:144114116"
ORIGIN      
        1 msrvasqeia gkyglggaar gsgdgssssn epslqrqlee lkrklkedmr kelkikegae
       61 nlrkattdrk slahvnsivk kansrlndlq rqlsdltadi lvtqghpspq gaqprstdsf
      121 ygdkgdmpls ttdqrlqsle kqlnielkvk qgaenmlqmy ssgpskdrkl laeaqqmqad
      181 skakidyirm mmarlrqnqd shsketgegc snnhldsttg gkipevmspv elqiedlrhr
      241 lkvecavveg aknvlklcqa agkvtdrkvf qeaqnslves sekldlirrs lelrraelsp
      301 gsakwqllkr elessqlvsp yspsivphgp raeqpgslas plsplaaspt islskpasvt
      361 gqlqvrlmgc qdlledvpgr lprsqvagvg adlrsslvra tgrgfsgrns srsysikeet
      421 sseimavlkl dnvhvgqtty kpcsqqawdq rftvaldrsr eleiqiywkd wrgmcavkfl
      481 rleefidder hgmalhlepk gllfaeirfq npmisrkprl qrqrklfihk gknflrptqm
      541 ninvatwgrl mkramppgcs dqvttvsppd allttapvst speldslvvt klsfedtnep
      601 dassplsshq tsletdvqsa lgefdflqqe tsitsmavee kaqqtsnmvv gghspspepi
      661 velapdeede vnklgeppqv tpvvvvpdve sgqgsltldh fefisvlgrg hfgkvilskh
      721 ktsgqyfaik alkkgdivar deleslmaek rifevansvq hpflvnlfac fqttnhvcfv
      781 meyacggdlm mhihtdifpe praafyaacv vlglqflhen riiyrdlkld nllldaegyv
      841 kladfglcke gmgygdrtgt fcgtpeflap evltetsytr avdwwglgvl vfemlvgesp
      901 fpgddeeevf dsivhdevry prflsieava imrrllrknp errlgtsekd aedvkkqaff
      961 rninwehllv kklkppfvpt vkspedvsnf ddeftceqpv ltapkdprpl tqeeqahfkd
     1021 ftytsnwc
//
LOCUS       XP_077503750            1028 aa            linear   INV 25-NOV-2025
DEFINITION  serine/threonine-protein kinase N isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503750
VERSION     XP_077503750.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647624.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1028
                     /product="serine/threonine-protein kinase N isoform X4"
                     /calculated_mol_wt=114612
     Region          35..100
                     /region_name="HR1_PKN_1"
                     /note="First Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11622"
                     /db_xref="CDD:212012"
     Site            order(43,46,49..50,53..54,56..57,60..61,63..64,79..80,83,
                     86..87,90)
                     /site_type="other"
                     /note="Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212012"
     Site            order(51..52,54..55,58..59,61..62,65..66,69,74..75,77)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212012"
     Region          130..199
                     /region_name="HR1_PKN_2"
                     /note="Second Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11623"
                     /db_xref="CDD:212013"
     Site            order(137,140,143..144,147..148,150..151,154..155,
                     157..158,178..179,182,185..186,189)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212013"
     Site            order(145..146,148..149,152..153,155..156,159..160,
                     173..174,176)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212013"
     Region          225..298
                     /region_name="HR1_PKN_3"
                     /note="Third Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11625"
                     /db_xref="CDD:212015"
     Site            order(235,238,241..242,245..246,248..249,252..253,
                     255..256,277..278,281,284..285,288)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212015"
     Site            order(243..244,246..247,250..251,253..254,257..258,
                     272..273,275)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212015"
     Region          422..504
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          701..1026
                     /region_name="STKc_PKN"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Protein Kinase N; cd05589"
                     /db_xref="CDD:270741"
     Site            order(707..711,715,728,730,765,781..782,784,788,790,826,
                     828,830..831,833,843..844,847,861..866,893,899,902)
                     /site_type="active"
                     /db_xref="CDD:270741"
     Site            order(707..711,715,728,730,765,782..784,788,826,828,
                     830..831,833,843..844)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270741"
     Site            order(711,788,790,826,828,830,847,861..866,893,899,902)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270741"
     Site            843..866
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270741"
     Site            1002
                     /site_type="other"
                     /note="turn motif phosphorylation site [posttranslational
                     modification]"
                     /db_xref="CDD:270741"
     Site            1018..1023
                     /site_type="other"
                     /note="hydrophobic motif (HM)"
                     /db_xref="CDD:270741"
     CDS             1..1028
                     /gene="Pkn"
                     /coded_by="XM_077647624.1:175..3261"
                     /db_xref="GeneID:144114116"
ORIGIN      
        1 msrvasqeia gkyglggaar gsgdgssssn epslqrqlee lkrklkedmr kelkikegae
       61 nlrkattdrk slahvnsivk kansrlndlq rqlsdltadi lvtqghpspq gaqprstdsf
      121 ygdkgdmpls ttdqrlqsle kqlnielkvk qgaenmlqmy ssgpskdrkl laeaqqmqad
      181 skakidyirm mmarlrqnqd shsketgegc snnhldsttg gkipevmspv elqiedlrhr
      241 lkvecavveg aknvlklcqa agkvtdrkvf qeaqnslves sekldlirrs lelrraelsp
      301 gsakwqllkr elessqlvsp yspsivphgp raeqpgslas plsplaaspt islskpasvt
      361 gqlqvrlmgc qdlledvpgr lprsqvagvg adlrsslvra tgrgfsgrns srsysikeet
      421 sseimavlkl dnvhvgqtty kpcsqqawdq rftvaldrsr eleiqiywkd wrgmcavkfl
      481 rleefidder hgmalhlepk gllfaeirfq npmisrkprl qrqrklfihk gknflrptqm
      541 ninvatwgrl mkramppgcs dqvttvsppd allttapvst speldslvvt klsfedtnep
      601 dassplsshq tsletdvqsa lgefdflqqe tsitsmavee kaqqtsnmvv gghspspepi
      661 velapdeede vnklgeppqv tpvvvvpdve sgqgsltldh fefisvlgrg hfgkvilskh
      721 ktsgqyfaik alkkgdivar deleslmaek rifevansvq hpflvnlfac fqttnhvcfv
      781 meyacggdlm mhihtdifpe praafyaacv vlglqflhen riiyrdlkld nllldaegyv
      841 kladfglcke gmgygdrtgt fcgtpeflap evltetsytr avdwwglgvl vfemlvgesp
      901 fpgddeeevf dsivhdevry prflsieava imrrllrknp errlgtsekd aedvkkqaff
      961 rninwehllv kklkppfvpt vkspedvsnf ddeftceqpv ltapkdprpl tqeeqahfkd
     1021 ftytsnwc
//
LOCUS       XP_077503751             496 aa            linear   INV 25-NOV-2025
DEFINITION  lariat debranching enzyme [Amblyomma americanum].
ACCESSION   XP_077503751
VERSION     XP_077503751.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647625.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..496
                     /product="lariat debranching enzyme"
                     /calculated_mol_wt=56204
     Region          3..258
                     /region_name="MPP_Dbr1_N"
                     /note="Dbr1 RNA lariat debranching enzyme, N-terminal
                     metallophosphatase domain; cd00844"
                     /db_xref="CDD:277322"
     Site            order(8,10,39,84..85,174,226,228)
                     /site_type="active"
                     /db_xref="CDD:277322"
     Site            order(39,84,174,226)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:277322"
     Region          243..372
                     /region_name="DBR1"
                     /note="Lariat debranching enzyme, C-terminal domain;
                     pfam05011"
                     /db_xref="CDD:461518"
     CDS             1..496
                     /gene="ldbr"
                     /coded_by="XM_077647625.1:150..1640"
                     /db_xref="GeneID:144114118"
ORIGIN      
        1 mkiavegcah geldkiyeti kglenqyqft vdlliicgdf qavrnasdme cmavpkkyqe
       61 mkdfhkyysg ekkaplltiv iggnheasny maelayggwl ceniyymgya svvningiri
      121 agisgiykgh dylkghfevp pyndstkrsa yhlrnlevfr mkqladpvdi vishdwprgi
      181 ynygntakll rqkkyftaev hsntlgcrpt eglleqlkpk ywfaahlhck faavvnhqdg
      241 mctkflaldk clprrdflqt ldmptssdep prltydlewl cvlqltdhll rvdsqnhymp
      301 gpgcgqrwqf tptkeekekl aatlnndllv pfnfertapv yvpgsercpg qvkpyinpqt
      361 ktfckrfglr dplaealnpq qqssplgisg flgnedvtst tmssslnese nlshqpspdt
      421 ddesvplhem saeerqkvht sefgspvcla edkkelfpel tertedvkpk tsplssgrvm
      481 krrnqsiyte teeedn
//
LOCUS       XP_077503752             333 aa            linear   INV 25-NOV-2025
DEFINITION  hypoxia-inducible factor 1-alpha inhibitor-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077503752
VERSION     XP_077503752.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647626.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..333
                     /product="hypoxia-inducible factor 1-alpha inhibitor-like
                     isoform X1"
                     /calculated_mol_wt=38421
     Region          48..288
                     /region_name="Cupin_8"
                     /note="Cupin-like domain; pfam13621"
                     /db_xref="CDD:463936"
     CDS             1..333
                     /gene="LOC144114119"
                     /coded_by="XM_077647626.1:45..1046"
                     /db_xref="GeneID:144114119"
ORIGIN      
        1 mahlsetwhs rannvqadfs dygipleivp rlsctdpean rlitsmmpvv ltdtnlvtta
       61 lkwdldylsk hfgngdctvy esdsvifkyy ddakvkehrl knfspptrrr emkmgqfver
      121 lknagrtkyy lqqamnetvg pnivldflgf nwewvtrqqk qngwgpltsn lllvategni
      181 tpthydeqqn ffaqlrghkr fllfspdqyk clyphpvwhp hdrqsqvdla srdldhfpla
      241 aqlhgweail gpgdvlylpm ywwhqvesap ccgytvsinf wyktapadkv iyplvghqkm
      301 aimrniekml vealkdvnev gpllramvlg ryl
//
LOCUS       XP_077503753             287 aa            linear   INV 25-NOV-2025
DEFINITION  hypoxia-inducible factor 1-alpha inhibitor-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077503753
VERSION     XP_077503753.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647627.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..287
                     /product="hypoxia-inducible factor 1-alpha inhibitor-like
                     isoform X2"
                     /calculated_mol_wt=33251
     Region          2..242
                     /region_name="Cupin_8"
                     /note="Cupin-like domain; pfam13621"
                     /db_xref="CDD:463936"
     CDS             1..287
                     /gene="LOC144114119"
                     /coded_by="XM_077647627.1:81..944"
                     /db_xref="GeneID:144114119"
ORIGIN      
        1 mpvvltdtnl vttalkwdld ylskhfgngd ctvyesdsvi fkyyddakvk ehrlknfspp
       61 trrremkmgq fverlknagr tkyylqqamn etvgpnivld flgfnwewvt rqqkqngwgp
      121 ltsnlllvat egnitpthyd eqqnffaqlr ghkrfllfsp dqykclyphp vwhphdrqsq
      181 vdlasrdldh fplaaqlhgw eailgpgdvl ylpmywwhqv esapccgytv sinfwyktap
      241 adkviyplvg hqkmaimrni ekmlvealkd vnevgpllra mvlgryl
//
LOCUS       XP_077503754             109 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114120 [Amblyomma americanum].
ACCESSION   XP_077503754
VERSION     XP_077503754.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647628.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..109
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..109
                     /product="uncharacterized protein LOC144114120"
                     /calculated_mol_wt=11983
     CDS             1..109
                     /gene="LOC144114120"
                     /coded_by="XM_077647628.1:190..519"
                     /db_xref="GeneID:144114120"
ORIGIN      
        1 mnckdtcppe psgdwdledv ctvlgvalll vvgllglplc lywdtrcvll tigslvvlcf
       61 varavherrr gtaalrerdh wlsltasflv rhsseerepl avtkcsysv
//
LOCUS       XP_077503755             754 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077503755
VERSION     XP_077503755.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647629.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..754
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=85440
     Region          86..209
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          305..709
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..754
                     /gene="LOC144114121"
                     /coded_by="XM_077647629.1:143..2407"
                     /db_xref="GeneID:144114121"
ORIGIN      
        1 mrtlgrnval lfgaavllag apltavaqna tdeepvtlad ildvtttkap epnmlermrk
       61 mvrdridesy dsmserflsa kisppcafkl lkfvhalrnf epwtlrmida sgkyptglfq
      121 gslsdlgayd qcietvvrde yggekvraqy cnvhiklgsd msffehvddv lrlshrrtpt
      181 fsrfqnseiv hgvkiglciv qectpediea vanvlivagd tkvkvtdcvt nvppeidttq
      241 aviigilgvl afimaastav dlylskrrrd eqrgtaltll ssfsivansk mlvkvttdkq
      301 sdayklrflh gmrffsigwv vlghsystfn ftnmarlvna lhygdnitfc fimagylsvd
      361 tflflagyll tynllrensn alwgtivaii rryirgtipi afcvmcmfla plmfrgpttp
      421 tlyelfyrev rtqfwalllq irnftgnlhi gmfghlwyms tdfqlfvisv iiiqffkkki
      481 wtsiivfgvl sliacsisaw qvsntiytpf vlpmadnfti mtgtlnkfyt hpsyhgvaff
      541 fgaitqlfly kyptvklskm lqaglwlmsa acgltailim ydwnrgehpa ewaklsfafs
      601 erllwsvwla wltfacstgr ggfintwlsw sawvpfsrls fgvyllhvpy yyirlnitre
      661 riffshytmv tqyfgalvas yllsyilfva ceaptgriek lvlmpkrrpq lhdnkstvpy
      721 medksdklhp emlykpkadm gsngnmapgq ssyl
//
LOCUS       XP_077503756             737 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077503756
VERSION     XP_077503756.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647630.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..737
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=82454
     Region          64..191
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          282..675
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..737
                     /gene="LOC144114122"
                     /coded_by="XM_077647630.1:510..2723"
                     /db_xref="GeneID:144114122"
ORIGIN      
        1 mklvhvlvva aavfgaavph vdaatrkksp elvetiksfv akmmnnpnsa ltqrllgaei
       61 spscmvgllk lmrgirnvep walrlfdasg kyptglfqgs madlgafdec vetlvldeyg
      121 nekirgqycn lylpaannse lleyvasgla mshprmidfa isssdssmve glrlgicfin
      181 dcsqediqmi inalaggtie lrvqdcvtne pqpwttaqis ivaflgtlav viaastvfdy
      241 citkrqikkg plqkalsafs ietnikllln ieqdkdsdas tyrflhgirf lsifsivlgh
      301 syaffdylam sravnalrya dmlgfnfimt aynsvdvfff isgflmlyni dkqaqtrvnr
      361 lyiyvvaiir rhlrttvpvf fvimccylgp viasgpnlkp lmerihyefs rqwwkillqv
      421 rnegerddqg flgvlwfisa dfqlfmvamp tvlilkrsyw gkiavfgimg avcsavgvwq
      481 lhgtafppfm mpfvtdikvl ndfysdvyvw pfhhglcyfa gcivcllvqk frhakisklk
      541 lfatwclcvf cgltcffiky dwcrgrnptd ewvkllvfva nllccaifvg wlvfacatgr
      601 ggiasrllsw kalvplsrls fgvycihvpi yyyyfalsre rvfyshftlv mlsfgvfvls
      661 nifsfitfia ceapvgslek qalmpsslph dskkrkaatt ngmlhngatv nnvpgrtgvn
      721 dktcmdavis lgdisrl
//
LOCUS       XP_077503757             747 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077503757
VERSION     XP_077503757.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647631.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..747
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=83651
     Region          71..197
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          291..682
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..747
                     /gene="LOC144114125"
                     /coded_by="XM_077647631.1:220..2463"
                     /db_xref="GeneID:144114125"
ORIGIN      
        1 mvsvkviplv clfvcapvsw cynesdtsga ttpqpeegiv aklqgflana lnkvspeltr
       61 klleaevspt csiglfklvg girrtepwal rlidamgkyp sglmqasktd lgafdecvet
      121 vvhdsfgeet irgqycnldi rlsenssfve atlaaiqlaf pkvvkfrghl tdrrlppirl
      181 gicvtndcne delqelvnkl lsspldikis ncvtslpipl trgqkailit igvlaamlvl
      241 gtitdicvss rkernavcgi lvrlltafsl isntktllqt vqdkssdayi lrffhglrff
      301 slvwivlghc ygaisptwsr llnnlimaeh tqdlivpaay iavdtfffls aylmchaisk
      361 qkrpavvvfi favirrfirt mvpvffvlmc myllpvmtsg pdakaffdqf ygemdrlwrd
      421 illqvqnyrk gaftilvhlw ylsvdfqlfl vtlpiviilk nrrkwaiavf vvlsivgcai
      481 aawqitgthv tpfvvvvtqs msiledtlnn fyflpfyhsv cyfsgciaff llqgfrdvkv
      541 pkivlavawl gaaglgvcci fskrpwyaar nptsdfgnic maffdritws lfllwitlac
      601 atgrggylns flswnafvpl srltfgvyli hapfiylvlh isrerilysh ftlvsmcfam
      661 lmwsyclayl afifceapta qldklifmgg grsdrrdrpa aqngtqhnek pwvvttemie
      721 ksqppmdlen gnnnahsgig nsvtyhl
//
LOCUS       XP_077503758             584 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114126 [Amblyomma americanum].
ACCESSION   XP_077503758
VERSION     XP_077503758.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647632.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..584
                     /product="uncharacterized protein LOC144114126"
                     /calculated_mol_wt=64608
     Region          4..110
                     /region_name="PH"
                     /note="Pleckstrin homology (PH) domain; cd00821"
                     /db_xref="CDD:275388"
     CDS             1..584
                     /gene="LOC144114126"
                     /coded_by="XM_077647632.1:226..1980"
                     /db_xref="GeneID:144114126"
ORIGIN      
        1 mviksgyvdv klppsarnss twkswkrkwv dltqlesasg qpcdvllevr anrnsgvqar
       61 gtlspktaqv frcgshsref avairsgqrp pmvflagese tqsqewmaal rallwppvpm
      121 velenvlgfk feasliddaw sgragllgsy gnlsisghkl ilthphtehv iqewylntlv
      181 rfqvfrnkhe adhgkllaie cgsesstgrg vlhfycpeal nfvytlrgvv qgiikrmppe
      241 qyswkeaqem rcppparren saqqrraaai edssgysvpk snaisvlhmp tfngplpppr
      301 meevqqgfpc srqshlsist fdsgisvtsg hvdnelespv sdvafsvssd ineriyekld
      361 dvllrrasle kerdippplp pkthrrrkse pakpavveea agevesspne rtlrpysmpa
      421 dmtpypkeeg thvspygtit kastnqvtqd nelpeqhakn adalqgaeeh imeestdcls
      481 ehgarkgdas vecegtavsl gdcatacqtf eestgdekes ghiscvleaa gdkyyfralr
      541 aggnledesg rkekrsysli acndhvrrqm srqrtfslps lsih
//
LOCUS       XP_077503759             168 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114127 [Amblyomma americanum].
ACCESSION   XP_077503759
VERSION     XP_077503759.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647633.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..168
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..168
                     /product="uncharacterized protein LOC144114127"
                     /calculated_mol_wt=18540
     Region          10..139
                     /region_name="MARVEL"
                     /note="Membrane-associating domain; cl04571"
                     /db_xref="CDD:383772"
     CDS             1..168
                     /gene="LOC144114127"
                     /coded_by="XM_077647633.1:180..686"
                     /db_xref="GeneID:144114127"
ORIGIN      
        1 mptigadrgf matangllri aeitlglcgl iciesisgec yyayihryef slfvvitsfl
       61 faffvimvfv lraqealarc fnvplallvs dsfialfyli vclleftayc pdpddnastk
      121 vagvfamfam mcfvvssyfs ykffqeerlp alpppglhaa tttqvlpk
//
LOCUS       XP_077503761             168 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114127 [Amblyomma americanum].
ACCESSION   XP_077503761
VERSION     XP_077503761.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647635.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..168
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..168
                     /product="uncharacterized protein LOC144114127"
                     /calculated_mol_wt=18540
     Region          10..139
                     /region_name="MARVEL"
                     /note="Membrane-associating domain; cl04571"
                     /db_xref="CDD:383772"
     CDS             1..168
                     /gene="LOC144114127"
                     /coded_by="XM_077647635.1:204..710"
                     /db_xref="GeneID:144114127"
ORIGIN      
        1 mptigadrgf matangllri aeitlglcgl iciesisgec yyayihryef slfvvitsfl
       61 faffvimvfv lraqealarc fnvplallvs dsfialfyli vclleftayc pdpddnastk
      121 vagvfamfam mcfvvssyfs ykffqeerlp alpppglhaa tttqvlpk
//
LOCUS       XP_077503762             168 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114127 [Amblyomma americanum].
ACCESSION   XP_077503762
VERSION     XP_077503762.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647636.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..168
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..168
                     /product="uncharacterized protein LOC144114127"
                     /calculated_mol_wt=18540
     Region          10..139
                     /region_name="MARVEL"
                     /note="Membrane-associating domain; cl04571"
                     /db_xref="CDD:383772"
     CDS             1..168
                     /gene="LOC144114127"
                     /coded_by="XM_077647636.1:162..668"
                     /db_xref="GeneID:144114127"
ORIGIN      
        1 mptigadrgf matangllri aeitlglcgl iciesisgec yyayihryef slfvvitsfl
       61 faffvimvfv lraqealarc fnvplallvs dsfialfyli vclleftayc pdpddnastk
      121 vagvfamfam mcfvvssyfs ykffqeerlp alpppglhaa tttqvlpk
//
LOCUS       XP_077503763             168 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114127 [Amblyomma americanum].
ACCESSION   XP_077503763
VERSION     XP_077503763.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647637.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..168
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..168
                     /product="uncharacterized protein LOC144114127"
                     /calculated_mol_wt=18540
     Region          10..139
                     /region_name="MARVEL"
                     /note="Membrane-associating domain; cl04571"
                     /db_xref="CDD:383772"
     CDS             1..168
                     /gene="LOC144114127"
                     /coded_by="XM_077647637.1:192..698"
                     /db_xref="GeneID:144114127"
ORIGIN      
        1 mptigadrgf matangllri aeitlglcgl iciesisgec yyayihryef slfvvitsfl
       61 faffvimvfv lraqealarc fnvplallvs dsfialfyli vclleftayc pdpddnastk
      121 vagvfamfam mcfvvssyfs ykffqeerlp alpppglhaa tttqvlpk
//
LOCUS       XP_077503764             168 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114127 [Amblyomma americanum].
ACCESSION   XP_077503764
VERSION     XP_077503764.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647638.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..168
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..168
                     /product="uncharacterized protein LOC144114127"
                     /calculated_mol_wt=18540
     Region          10..139
                     /region_name="MARVEL"
                     /note="Membrane-associating domain; cl04571"
                     /db_xref="CDD:383772"
     CDS             1..168
                     /gene="LOC144114127"
                     /coded_by="XM_077647638.1:147..653"
                     /db_xref="GeneID:144114127"
ORIGIN      
        1 mptigadrgf matangllri aeitlglcgl iciesisgec yyayihryef slfvvitsfl
       61 faffvimvfv lraqealarc fnvplallvs dsfialfyli vclleftayc pdpddnastk
      121 vagvfamfam mcfvvssyfs ykffqeerlp alpppglhaa tttqvlpk
//
LOCUS       XP_077503765             168 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114127 [Amblyomma americanum].
ACCESSION   XP_077503765
VERSION     XP_077503765.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647639.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..168
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..168
                     /product="uncharacterized protein LOC144114127"
                     /calculated_mol_wt=18540
     Region          10..139
                     /region_name="MARVEL"
                     /note="Membrane-associating domain; cl04571"
                     /db_xref="CDD:383772"
     CDS             1..168
                     /gene="LOC144114127"
                     /coded_by="XM_077647639.1:556..1062"
                     /db_xref="GeneID:144114127"
ORIGIN      
        1 mptigadrgf matangllri aeitlglcgl iciesisgec yyayihryef slfvvitsfl
       61 faffvimvfv lraqealarc fnvplallvs dsfialfyli vclleftayc pdpddnastk
      121 vagvfamfam mcfvvssyfs ykffqeerlp alpppglhaa tttqvlpk
//
LOCUS       XP_077503766             109 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114128 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503766
VERSION     XP_077503766.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647640.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..109
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..109
                     /product="uncharacterized protein LOC144114128 isoform X1"
                     /calculated_mol_wt=11969
     CDS             1..109
                     /gene="LOC144114128"
                     /coded_by="XM_077647640.1:1102..1431"
                     /db_xref="GeneID:144114128"
ORIGIN      
        1 mivdnssivh llplnwalid tvlwevlall flvsssllmh skvlyrqtyf fivdwtcslf
       61 vvsgifglgc mcsclalgiv rflrpksplq clfpaaasqd immspitas
//
LOCUS       XP_077503767             109 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114128 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503767
VERSION     XP_077503767.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647641.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..109
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..109
                     /product="uncharacterized protein LOC144114128 isoform X1"
                     /calculated_mol_wt=11969
     CDS             1..109
                     /gene="LOC144114128"
                     /coded_by="XM_077647641.1:614..943"
                     /db_xref="GeneID:144114128"
ORIGIN      
        1 mivdnssivh llplnwalid tvlwevlall flvsssllmh skvlyrqtyf fivdwtcslf
       61 vvsgifglgc mcsclalgiv rflrpksplq clfpaaasqd immspitas
//
LOCUS       XP_077503768              87 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114128 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503768
VERSION     XP_077503768.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647642.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..87
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..87
                     /product="uncharacterized protein LOC144114128 isoform X2"
                     /calculated_mol_wt=9343
     CDS             1..87
                     /gene="LOC144114128"
                     /coded_by="XM_077647642.1:1102..1365"
                     /db_xref="GeneID:144114128"
ORIGIN      
        1 mivdnssivh llplnwalid tvlwevlall flvsssllmh skifglgcmc sclalgivrf
       61 lrpksplqcl fpaaasqdim mspitas
//
LOCUS       XP_077503769              87 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114128 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503769
VERSION     XP_077503769.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647643.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..87
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..87
                     /product="uncharacterized protein LOC144114128 isoform X2"
                     /calculated_mol_wt=9343
     CDS             1..87
                     /gene="LOC144114128"
                     /coded_by="XM_077647643.1:613..876"
                     /db_xref="GeneID:144114128"
ORIGIN      
        1 mivdnssivh llplnwalid tvlwevlall flvsssllmh skifglgcmc sclalgivrf
       61 lrpksplqcl fpaaasqdim mspitas
//
LOCUS       XP_077503771             809 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114133 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503771
VERSION     XP_077503771.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647645.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..809
                     /product="uncharacterized protein LOC144114133 isoform X1"
                     /calculated_mol_wt=93277
     Region          <18..371
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          38..>202
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          38..73
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          80..121
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          127..166
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          174..215
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          220..247
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          248..291
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          298..339
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          347..369
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          561..>775
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     CDS             1..809
                     /gene="LOC144114133"
                     /coded_by="XM_077647645.1:158..2587"
                     /db_xref="GeneID:144114133"
ORIGIN      
        1 maskspqknm smkdlkalat krlgvfavkh lpkdtsyvvg lsqsvssnki aaassdnsiy
       61 lydeylmqkg ilqdkmkevt gvrfsisnpn lvynscrdgs vrvwdlrnir hpvskfvant
      121 egkvkplscf dfncneqflc agtdlyddns yllfwdfrsn kilggywdsh sdditqikfh
      181 pskpncmasa smdslvnlfd isqtdeddal qatlncdcsi nkmqwvrqrs geelisvitt
      241 devvqiwspd eagggpgplc tsctpspsav iqiilevsar kvitrsdlts tfsepvridh
      301 ivdnvmfedk myitagsskg lgllwnvtdq pelisvlwrg hrdlprvsal tkyfvvtgge
      361 dgnvciwrpe secppqaeee esedevddns rtsvdrddks dhsrdwsrsr srsrsrsrsr
      421 rrsrdrsrdr srdrsrersr srgrsrgrsk drgrsrgrsr drsrnrsrgr srgrsrdrsr
      481 srdrgkssrd rsrgrsrgrs rdrsrgrsrd rsraksrdrs rgrsrdrsra rsrdrsrgrs
      541 rdrsrgrsrd rsrgrsrdrs kgrsrdrsgg rsrnrskdra rdrskakdrr srdrsrdkhr
      601 serdrskdrs srngkdkpdr srsdksrsdr dksdrdksdr drsdrdksdk dkseknksdr
      661 dkdkersdks rsdksksekd rsdkdksere keksdrdsks drnrsdkers eksdkgksdr
      721 drskdrsdvk enstsernkt sdrdksdrer tdgdksdren sykdrsdges ksdgdkstsk
      781 sdrsdkssyk mpssvkekls shnskpysr
//
LOCUS       XP_077503772             784 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114133 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503772
VERSION     XP_077503772.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647646.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..784
                     /product="uncharacterized protein LOC144114133 isoform X2"
                     /calculated_mol_wt=90927
     Region          38..>202
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          38..73
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          80..121
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          127..166
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          174..215
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          220..247
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          273..314
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          322..344
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          536..>750
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     CDS             1..784
                     /gene="LOC144114133"
                     /coded_by="XM_077647646.1:158..2512"
                     /db_xref="GeneID:144114133"
ORIGIN      
        1 maskspqknm smkdlkalat krlgvfavkh lpkdtsyvvg lsqsvssnki aaassdnsiy
       61 lydeylmqkg ilqdkmkevt gvrfsisnpn lvynscrdgs vrvwdlrnir hpvskfvant
      121 egkvkplscf dfncneqflc agtdlyddns yllfwdfrsn kilggywdsh sdditqikfh
      181 pskpncmasa smdslvnlfd isqtdeddal qatlncdcsi nkmqwvrqrs geelisvitt
      241 devvqiwspd evsarkvitr sdltstfsep vridhivdnv mfedkmyita gsskglgllw
      301 nvtdqpelis vlwrghrdlp rvsaltkyfv vtggedgnvc iwrpesecpp qaeeeesede
      361 vddnsrtsvd rddksdhsrd wsrsrsrsrs rsrsrrrsrd rsrdrsrdrs rersrsrgrs
      421 rgrskdrgrs rgrsrdrsrn rsrgrsrgrs rdrsrsrdrg kssrdrsrgr srgrsrdrsr
      481 grsrdrsrak srdrsrgrsr drsrarsrdr srgrsrdrsr grsrdrsrgr srdrskgrsr
      541 drsggrsrnr skdrardrsk akdrrsrdrs rdkhrserdr skdrssrngk dkpdrsrsdk
      601 srsdrdksdr dksdrdrsdr dksdkdksek nksdrdkdke rsdksrsdks ksekdrsdkd
      661 kserekeksd rdsksdrnrs dkerseksdk gksdrdrskd rsdvkensts ernktsdrdk
      721 sdrertdgdk sdrensykdr sdgesksdgd kstsksdrsd kssykmpssv keklsshnsk
      781 pysr
//
LOCUS       XP_077503773             437 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077503773
VERSION     XP_077503773.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647647.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..437
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=49002
     Region          1..437
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..437
                     /gene="LOC144114134"
                     /coded_by="XM_077647647.1:44..1357"
                     /db_xref="GeneID:144114134"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevicdehgi dpsgayygds dlqlerinvy ynetsggkyv
       61 prailvdlep gtmdavrsgr fgqlfrpdnf vfgqsgagnn wakghytega elvdsvldvv
      121 rkeaescdcm qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdetf cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrgcqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkdssy fvewipnnvk tavcdipprg
      361 lkmsatfian staiqelfkr iseqftamfr rkaflhwytg egmdelefte aesnlnelvs
      421 eyqqyqeatv edegefe
//
LOCUS       XP_077503774             415 aa            linear   INV 25-NOV-2025
DEFINITION  mitogen-activated protein kinase dJNK [Amblyomma americanum].
ACCESSION   XP_077503774
VERSION     XP_077503774.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647648.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..415
                     /product="mitogen-activated protein kinase dJNK"
                     /calculated_mol_wt=47268
     Region          23..359
                     /region_name="STKc_JNK"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     c-Jun N-terminal Kinase; cd07850"
                     /db_xref="CDD:270840"
     Site            order(30..36,38,51,53,70,84,106..112,114..115,149,151,
                     153..154,156,166..167,170,181,183..186,188,225)
                     /site_type="active"
                     /db_xref="CDD:270840"
     Site            order(30..36,38,51,53,84,106..112,156,166)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270840"
     Site            order(70,114,149,151,170,181,183..186,188,225)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270840"
     Site            order(110,116,125,128,157..161,322..323,325,328)
                     /site_type="other"
                     /note="KIM docking site [polypeptide binding]"
                     /db_xref="CDD:270840"
     Site            order(166..176,180..188)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270840"
     CDS             1..415
                     /gene="bsk"
                     /coded_by="XM_077647648.1:279..1526"
                     /db_xref="GeneID:144114135"
ORIGIN      
        1 mskhsslfyt fevgdtkfti lrryqnlksi gsgaqgivca aydsvtgqnv aikklsrpfq
       61 nvthakrayr efklmklvnh kniigllnaf tpqktleefq dvylvmelmd anlcqviqmd
      121 ldhermsyll yqmlcgikhl hlagiihrdl kpsnivvksd ctlkildfgl artagttfmm
      181 tpyvvtryyr apevilgmgy kenvdiwsvg cimgemirgs vlfpgtdhid qwnkiieqlg
      241 tpsqefmrrl qptvrnyven rpryagysfd klfpdvlfpt dshehsrlka sqardllskm
      301 lvidperris vddalshpyi nvwydeaevn apapasydhs vderehtveq wkeliykevl
      361 eyeqthnslg tpgrnalhgs sqdgaievid gdqsvdqssn ggasansyhr rryqs
//
LOCUS       XP_077503775             335 aa            linear   INV 25-NOV-2025
DEFINITION  serine/threonine-protein kinase H1 homolog isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503775
VERSION     XP_077503775.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647649.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..335
                     /product="serine/threonine-protein kinase H1 homolog
                     isoform X1"
                     /calculated_mol_wt=36353
     Region          61..282
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(69..72,75,77,90,92,118,134..137,180,184..185,187,
                     200..201)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270989"
     CDS             1..335
                     /gene="LOC144114136"
                     /coded_by="XM_077647649.1:67..1074"
                     /db_xref="GeneID:144114136"
ORIGIN      
        1 mgsgqakmtq kaaalsagsv qtvmrpgchq rkqqgkwvkt ikissfssla gsyridarvl
       61 akynvktvis kgrfgrvlrv enklskqlya vkiietevds hvetelavlr rvrhtnvvrl
      121 devirvgglv ymvmelatgg slqdklevha pfvetdaarv ihmltcglaq lhglgiahrn
      181 lepenvlyeh pgagakvlit efglasapta greacmhtvc egklrymape mvsrrpytha
      241 vdmwalgvit hvlltgvlpf dahsdaavvq limgaqlsva peanssfpts plpertgrrp
      301 csdarsylpn gpcfgapaqr srptgyrneg lrlvl
//
LOCUS       XP_077503776             315 aa            linear   INV 25-NOV-2025
DEFINITION  serine/threonine-protein kinase H1 homolog isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503776
VERSION     XP_077503776.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647650.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..315
                     /product="serine/threonine-protein kinase H1 homolog
                     isoform X2"
                     /calculated_mol_wt=34154
     Region          61..284
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(69..72,75,77,90,92,118,134..137,180,184..185,187,
                     200..201)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270989"
     CDS             1..315
                     /gene="LOC144114136"
                     /coded_by="XM_077647650.1:67..1014"
                     /db_xref="GeneID:144114136"
ORIGIN      
        1 mgsgqakmtq kaaalsagsv qtvmrpgchq rkqqgkwvkt ikissfssla gsyridarvl
       61 akynvktvis kgrfgrvlrv enklskqlya vkiietevds hvetelavlr rvrhtnvvrl
      121 devirvgglv ymvmelatgg slqdklevha pfvetdaarv ihmltcglaq lhglgiahrn
      181 lepenvlyeh pgagakvlit efglasapta greacmhtvc egklrymape mvsrrpytha
      241 vdmwalgvit hvlltgvlpf dahsdaavvq limgaqlsva pedweaallg cqelsaeral
      301 lrrtcaavkt ngise
//
LOCUS       XP_077503777             315 aa            linear   INV 25-NOV-2025
DEFINITION  serine/threonine-protein kinase H1 homolog [Amblyomma americanum].
ACCESSION   XP_077503777
VERSION     XP_077503777.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647651.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..315
                     /product="serine/threonine-protein kinase H1 homolog"
                     /calculated_mol_wt=34299
     Region          61..284
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(69..72,75,77,90,92,118,134..137,180,184..185,187,
                     200..201)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270989"
     CDS             1..315
                     /gene="LOC144114137"
                     /coded_by="XM_077647651.1:146..1093"
                     /db_xref="GeneID:144114137"
ORIGIN      
        1 mgsgqakmtq ktaalsagsv qtvmrpgchq rkqqgkwvkt ikissfssla gsyridarvl
       61 akynvktvis kgrfgrvlrv enklskqlya vkiietevds hvetelavlr rvrhtnvvrl
      121 devfrvgglv yvvmelatgg slqdklemha pfveteaarv ihmltcglah lhslgiahrn
      181 lepenvlyeh pgagakvlit efglasapta greacmhtvc egklrymape mvsrrpytha
      241 vdmwalgvit hvlltgvlpf dahsdaavvr limgaqlsva pedweaallg cqelsaeral
      301 lrrtcaavkt ngise
//
LOCUS       XP_077503778             312 aa            linear   INV 25-NOV-2025
DEFINITION  serine/threonine-protein kinase H1 homolog [Amblyomma americanum].
ACCESSION   XP_077503778
VERSION     XP_077503778.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647652.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..312
                     /product="serine/threonine-protein kinase H1 homolog"
                     /calculated_mol_wt=33912
     Region          58..281
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(66..69,72,74,87,89,115,131..134,177,181..182,184,
                     197..198)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270989"
     CDS             1..312
                     /gene="LOC144114138"
                     /coded_by="XM_077647652.1:117..1055"
                     /db_xref="GeneID:144114138"
ORIGIN      
        1 mgsgqakmtq ktaalsagsv etvmrpgchq rkqqgkwvkt ikisslagsy ridarvlaky
       61 nvktviskgr fgrvlrvenk lskqlyavki ietevdshve telavlrrvr hsnvvrldev
      121 frvgglvymv melatggslq dklevrapfv etdaarvihm ltcglahlhg lgiahrnlep
      181 envlyehpga gakvlitefg lasaptagre acmhtvcegk lrymapemvs rrpythavdm
      241 walgvithvl ltgvlpfdah sdaavvqlim gaqlsvaped weaallgcqe lsaerallrr
      301 tcaavktngi se
//
LOCUS       XP_077503779             295 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase MARCHF8-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503779
VERSION     XP_077503779.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647653.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..295
                     /product="E3 ubiquitin-protein ligase MARCHF8-like isoform
                     X1"
                     /calculated_mol_wt=32599
     Region          71..136
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(73,76,92,97,100,103,116,119)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     CDS             1..295
                     /gene="LOC144114141"
                     /coded_by="XM_077647653.1:164..1051"
                     /db_xref="GeneID:144114141"
ORIGIN      
        1 msleplppgr hvdtsghqas smeqpspaae elckqqppem qqeapppapt vqqqrrpssq
       61 psngqtngng dmcrichcea daenplispc ycagslryvh qaclqqwiks sdtrccelck
      121 fdfimhtkik pfrkweklem ssveqrkvlc sitfhvvait cvvwslyvli drtaeemreg
      181 nldwpfwtkl ivvaigftgg lvfmyvqckm yvqlfrrwra fnrviyvqda pvpdkgaalp
      241 taadlsppvt vqgttsskrp pasacaplhd gpteitvakd sspdtpgpde praas
//
LOCUS       XP_077503780             274 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase MARCHF8-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503780
VERSION     XP_077503780.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647654.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..274
                     /product="E3 ubiquitin-protein ligase MARCHF8-like isoform
                     X2"
                     /calculated_mol_wt=30414
     Region          45..108
                     /region_name="RING_CH-C4HC3_MARCH8"
                     /note="RING-CH finger, H2 subclass (C4HC3-type), found in
                     membrane-associated RING-CH8 (MARCH8); cd16807"
                     /db_xref="CDD:438458"
     CDS             1..274
                     /gene="LOC144114141"
                     /coded_by="XM_077647654.1:289..1113"
                     /db_xref="GeneID:144114141"
ORIGIN      
        1 meqpspaaee lckqqppemq qeapppaptv qqqrrpssqp sngqtngngd mcrichcead
       61 aenplispcy cagslryvhq aclqqwikss dtrccelckf dfimhtkikp frkweklems
      121 sveqrkvlcs itfhvvaitc vvwslyvlid rtaeemregn ldwpfwtkli vvaigftggl
      181 vfmyvqckmy vqlfrrwraf nrviyvqdap vpdkgaalpt aadlsppvtv qgttsskrpp
      241 asacaplhdg pteitvakds spdtpgpdep raas
//
LOCUS       XP_077503781             274 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase MARCHF8-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503781
VERSION     XP_077503781.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647655.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..274
                     /product="E3 ubiquitin-protein ligase MARCHF8-like isoform
                     X2"
                     /calculated_mol_wt=30414
     Region          45..108
                     /region_name="RING_CH-C4HC3_MARCH8"
                     /note="RING-CH finger, H2 subclass (C4HC3-type), found in
                     membrane-associated RING-CH8 (MARCH8); cd16807"
                     /db_xref="CDD:438458"
     CDS             1..274
                     /gene="LOC144114141"
                     /coded_by="XM_077647655.1:350..1174"
                     /db_xref="GeneID:144114141"
ORIGIN      
        1 meqpspaaee lckqqppemq qeapppaptv qqqrrpssqp sngqtngngd mcrichcead
       61 aenplispcy cagslryvhq aclqqwikss dtrccelckf dfimhtkikp frkweklems
      121 sveqrkvlcs itfhvvaitc vvwslyvlid rtaeemregn ldwpfwtkli vvaigftggl
      181 vfmyvqckmy vqlfrrwraf nrviyvqdap vpdkgaalpt aadlsppvtv qgttsskrpp
      241 asacaplhdg pteitvakds spdtpgpdep raas
//
LOCUS       XP_077503783             274 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase MARCHF8-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503783
VERSION     XP_077503783.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647657.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..274
                     /product="E3 ubiquitin-protein ligase MARCHF8-like isoform
                     X2"
                     /calculated_mol_wt=30414
     Region          45..108
                     /region_name="RING_CH-C4HC3_MARCH8"
                     /note="RING-CH finger, H2 subclass (C4HC3-type), found in
                     membrane-associated RING-CH8 (MARCH8); cd16807"
                     /db_xref="CDD:438458"
     CDS             1..274
                     /gene="LOC144114141"
                     /coded_by="XM_077647657.1:419..1243"
                     /db_xref="GeneID:144114141"
ORIGIN      
        1 meqpspaaee lckqqppemq qeapppaptv qqqrrpssqp sngqtngngd mcrichcead
       61 aenplispcy cagslryvhq aclqqwikss dtrccelckf dfimhtkikp frkweklems
      121 sveqrkvlcs itfhvvaitc vvwslyvlid rtaeemregn ldwpfwtkli vvaigftggl
      181 vfmyvqckmy vqlfrrwraf nrviyvqdap vpdkgaalpt aadlsppvtv qgttsskrpp
      241 asacaplhdg pteitvakds spdtpgpdep raas
//
LOCUS       XP_077503784             274 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase MARCHF8-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503784
VERSION     XP_077503784.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647658.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..274
                     /product="E3 ubiquitin-protein ligase MARCHF8-like isoform
                     X2"
                     /calculated_mol_wt=30414
     Region          45..108
                     /region_name="RING_CH-C4HC3_MARCH8"
                     /note="RING-CH finger, H2 subclass (C4HC3-type), found in
                     membrane-associated RING-CH8 (MARCH8); cd16807"
                     /db_xref="CDD:438458"
     CDS             1..274
                     /gene="LOC144114141"
                     /coded_by="XM_077647658.1:159..983"
                     /db_xref="GeneID:144114141"
ORIGIN      
        1 meqpspaaee lckqqppemq qeapppaptv qqqrrpssqp sngqtngngd mcrichcead
       61 aenplispcy cagslryvhq aclqqwikss dtrccelckf dfimhtkikp frkweklems
      121 sveqrkvlcs itfhvvaitc vvwslyvlid rtaeemregn ldwpfwtkli vvaigftggl
      181 vfmyvqckmy vqlfrrwraf nrviyvqdap vpdkgaalpt aadlsppvtv qgttsskrpp
      241 asacaplhdg pteitvakds spdtpgpdep raas
//
LOCUS       XP_077503785             274 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase MARCHF8-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503785
VERSION     XP_077503785.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647659.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..274
                     /product="E3 ubiquitin-protein ligase MARCHF8-like isoform
                     X2"
                     /calculated_mol_wt=30414
     Region          45..108
                     /region_name="RING_CH-C4HC3_MARCH8"
                     /note="RING-CH finger, H2 subclass (C4HC3-type), found in
                     membrane-associated RING-CH8 (MARCH8); cd16807"
                     /db_xref="CDD:438458"
     CDS             1..274
                     /gene="LOC144114141"
                     /coded_by="XM_077647659.1:313..1137"
                     /db_xref="GeneID:144114141"
ORIGIN      
        1 meqpspaaee lckqqppemq qeapppaptv qqqrrpssqp sngqtngngd mcrichcead
       61 aenplispcy cagslryvhq aclqqwikss dtrccelckf dfimhtkikp frkweklems
      121 sveqrkvlcs itfhvvaitc vvwslyvlid rtaeemregn ldwpfwtkli vvaigftggl
      181 vfmyvqckmy vqlfrrwraf nrviyvqdap vpdkgaalpt aadlsppvtv qgttsskrpp
      241 asacaplhdg pteitvakds spdtpgpdep raas
//
LOCUS       XP_077503786             206 aa            linear   INV 25-NOV-2025
DEFINITION  serine/threonine-protein kinase H2-like [Amblyomma americanum].
ACCESSION   XP_077503786
VERSION     XP_077503786.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647660.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..206
                     /product="serine/threonine-protein kinase H2-like"
                     /calculated_mol_wt=22140
     Region          <1..186
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     CDS             1..206
                     /gene="LOC144114142"
                     /coded_by="XM_077647660.1:88..708"
                     /db_xref="GeneID:144114142"
ORIGIN      
        1 mvmelatggs lqdklevrap fvetdaarvi hmltcglahl hglgiahrnl epenvlyehp
       61 gagakvlite fglasaptag reacmhtvce gklrymapem vsrrpythav dmwalgvith
      121 vlltgvlpfd ahsdaavvql imgaqlsvap eawehvseha qsvvdqllqh nashrpsaaq
      181 llehpwfaaa ttarmmsapp qtrsdp
//
LOCUS       XP_077503787             149 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114143 [Amblyomma americanum].
ACCESSION   XP_077503787
VERSION     XP_077503787.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647661.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..149
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..149
                     /product="uncharacterized protein LOC144114143"
                     /calculated_mol_wt=15970
     CDS             1..149
                     /gene="LOC144114143"
                     /coded_by="XM_077647661.1:106..555"
                     /db_xref="GeneID:144114143"
ORIGIN      
        1 masttttvrt tvvregptik fvsvqqgfis tisglltaie mvlgiivfsl amlwltrgav
       61 lflilisftf wimsflilas safsatgtll pttlfymvfh ctaflfylsg gvstiissyh
      121 gvtiaagvlg lvasvfhlih tgfaykkki
//
LOCUS       XP_077503788             727 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114144 [Amblyomma americanum].
ACCESSION   XP_077503788
VERSION     XP_077503788.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647662.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..727
                     /product="uncharacterized protein LOC144114144"
                     /calculated_mol_wt=77671
     Region          7..82
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cl49608"
                     /db_xref="CDD:483948"
     Site            order(15..21,33,36,64,68,71..72)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467153"
     Region          <583..>690
                     /region_name="PRK07003"
                     /note="DNA polymerase III subunit gamma/tau"
                     /db_xref="CDD:235906"
     CDS             1..727
                     /gene="LOC144114144"
                     /coded_by="XM_077647662.1:315..2498"
                     /db_xref="GeneID:144114144"
ORIGIN      
        1 magsaitvrl aggspwgfrl qggrgtgqpl titktrrksk ayqvllegdq lisingqpcd
       61 hlshdeamgi vesgcpelvi evrrgaehnk enestpkdnp hpkietqaaa aapvwtaptg
      121 tsffpkispt gsgafskgll tstsalprtg ptlvgplsdl gnipkpfvpf qdtspgslre
      181 vkssvehird patgvtkevh ttiekesket psslfrsvte tkketsqlps ggsllsattt
      241 vtttssssss ttkkapppta pkpklklnte lasdvarfgf ppdrpcssas easgqpaqqa
      301 ggsqdsplls ptglvllpkf yeppaynsam dvhplslhrk ktfssssfye epnciyptve
      361 eqvelarkia dslsadtnrk srganmffkr vkrshkwvhe apdysdseat lsgtetsrde
      421 latpdpatvp ykiskgppkl klildprhlq dantlrstgi sivehnaisp evcldlvkdl
      481 nspcgkgaam fakrkkksed wvvdvervka qlgdrwpeph qpvapirtpi ktpvdrfkes
      541 lvnprlrlvk spweaalesp igscekafam vrpeevvesv iraaeakgvp epapyqngla
      601 gahepaayep rppttpynpt dpclaraprg wrgtatqpap epsggcwpsv gasavpsasv
      661 spcpplqpsg vvqpsalspr pafgspapsa islsqfqnfn slprswsprc ssqatfrpvk
      721 apaffar
//
LOCUS       XP_077503789             132 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114145 [Amblyomma americanum].
ACCESSION   XP_077503789
VERSION     XP_077503789.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647663.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..132
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..132
                     /product="uncharacterized protein LOC144114145"
                     /calculated_mol_wt=14329
     CDS             1..132
                     /gene="LOC144114145"
                     /coded_by="XM_077647663.1:134..532"
                     /db_xref="GeneID:144114145"
ORIGIN      
        1 meestkvplt pgsvinvplg arlemqlklp teggivvpsd catadegqtv lkkstqsrte
       61 tqqskrvath gdqtvtessq avrklmqdsr smvhqsstes nesqrivktt aqqqmkyskq
      121 tfsstssfes hn
//
LOCUS       XP_077503790             486 aa            linear   INV 25-NOV-2025
DEFINITION  synaptotagmin-15-like [Amblyomma americanum].
ACCESSION   XP_077503790
VERSION     XP_077503790.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647664.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..486
                     /product="synaptotagmin-15-like"
                     /calculated_mol_wt=52654
     Region          212..337
                     /region_name="C2A_Synaptotagmin-15-17"
                     /note="C2A domain first repeat present in Synaptotagmins
                     15 and 17; cd08390"
                     /db_xref="CDD:176036"
     Region          347..485
                     /region_name="C2B_Synaptotagmin"
                     /note="C2 domain second repeat present in Synaptotagmin;
                     cd00276"
                     /db_xref="CDD:175975"
     CDS             1..486
                     /gene="LOC144114146"
                     /coded_by="XM_077647664.1:125..1585"
                     /db_xref="GeneID:144114146"
ORIGIN      
        1 maelqrassp plverappvq vsassvqgsa gllqsaalpg alaaaaacll lalsvacllq
       61 rrrrkadvqp tdqgtyerld cgrsprvsia vagpneprnt psptsrrslr rgtnephfyg
      121 gciaqerpid fvvptlagss lcgsaaslvs dvsscaaepg qpcsspgdag pvsllpvsts
      181 llgglnpely rvtaadgadd geertfpesh rgriwfslqy edpreslelr vikarnlpsr
      241 vcgsvnccdp fvriqllpde rryqqtkckk ktcnpifeet yifqipakal serilkltvl
      301 dndrgkrhnv ighvlfplrd ldplsgeriv awrdlettvd lappdqgell lglcykagle
      361 rltvtvlear glvapptmgn hasfdtqvkv tfllenkavk skktsvckrt sepkfmesfa
      421 frltpaglsa asvilkltcl appykdrclg rvvlgsfmfa rglgqqhwtd alaaapkqin
      481 rwhrls
//
LOCUS       XP_077503791            8736 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503791
VERSION     XP_077503791.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647665.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8736
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..8736
                     /product="uncharacterized protein LOC144114148 isoform X1"
                     /calculated_mol_wt=938561
     Region          59..90
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          73..390
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(92,94,98..99,102..104,106..107,111,114,123,125,127,
                     131..132,135..137,139..140,144,147,156,158,160,164..165,
                     168..170,172..173,177,180)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          92..123
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          125..156
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          158..183
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          191..216
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          224..251
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(253,255,259..260,263..265,267..268,272,275,284,286,
                     288,292..293,296..298,300..301,305,308,317,319,321,
                     325..326,329..331,333..334,338,341)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          253..284
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          286..317
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          319..349
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          333..621
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          352..416
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          418..449
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(451,453,457..458,461..463,465..466,470,473,482,484,
                     486,490..491,494..496,498..499,503,506,515,517,519,
                     523..524,527..529,531..532,536,539)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          451..482
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          484..515
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          499..777
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          517..546
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          550..575
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          583..614
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          616..649
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          650..680
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          682..712
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(715,717,721..722,725..727,729..730,734,737,746,748,
                     750,754..755,758..760,762..763,767,770,779,781,783,
                     787..788,791..793,795..796,800,803)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          715..746
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          748..779
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          753..834
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          781..811
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          999..1101
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1324..1456
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1488..1571
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <1885..2136
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          <4499..4759
                     /region_name="YbbR"
                     /note="Cyclic di-AMP synthase regulator CdaR, YbbR domain
                     [Signal transduction mechanisms]; COG4856"
                     /db_xref="CDD:443884"
     Region          <7164..7477
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..8736
                     /gene="LOC144114148"
                     /coded_by="XM_077647665.1:884..27094"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 mgpgeptirq sreswigcli rllasqndqc dpsvylflrw ltllllravl sqmmppatdg
       61 nasflraara gnlekvldyl kgsidintsn anglnalhla akeghvnvvs ellkrganvn
      121 attkkgnsal hiaslagqee vvkllvqkqa nvnvqsqngf tplymaaqen hdgvvrflla
      181 nganqslate dgftplaval qqghdkvvav llendargkv rlpalhiask kddckaaall
      241 lhsehnpdvt sksgftplhi aahygnsnia sllldkgadv nfpakhqitp lhvaakwgks
      301 nmvklllekg akidastrdg ltplhcaars ghdqvveqll eknapitakt knglaplhma
      361 sqgdhvdsar illyhkapvd dvtvdyltal hvaahcghvg vakllldrra dpnaralngf
      421 tplhiackkn rikvvelllk hgasieatte sgltplhvas fmgcmniviy liqhganpdi
      481 ptvrgetplh laaranqtdi irillrngah vdakarelqt alhiasrlgn admvglllqh
      541 gaavdaptkd aytplhvaar egqdevaall ldhgaalaap tkkgftplhl aakygnlkva
      601 qlllqkdapv daqgkngvtp lhvaahydhv nvallllekg asphsaarng ytplhvaark
      661 dqmdiassll eygakpgaes ragftplhla aqeghadlaa llvehgadcd akakngltpm
      721 hlcaqedrve vatilakhga sldpttkagy tplhvachfg qtnmirfllr qganvnatts
      781 hgytplhqaa qqghtliinl llehraapna vtnqgqtala iaqrlgyisv vetlkvvtet
      841 ivttttttvt eekyrvvape tmhetfmsds edeaaednml gdqsfrylta demkslgdds
      901 lpidvtrder itesihitre pghpapltqe eerlsptqah tteaifvgny apdnvdlsrt
      961 pihagsllsw dgdscssayv lsptkrmwre ssklkwktfl vsflvdargg amrgcrhsgv
     1021 rviipprkaq mpmritcryl rkeklphppp llegeacasr ilevgpagak flgpvilevp
     1081 hfaslrgker eisilrsdng etwkehtlea seeavqevln esfegeelsa ledlqtnriv
     1141 rilttdfpqy favvsrtrqe vhavgpeggl lsstvvpqvq aifpegaltk kirvglqaqp
     1201 ipaelvtkll ghrvavspiv tveprrrkfh kpitltipvp qaatkgminq yagdaptlrl
     1261 lcsitggtnk aqwedvtgst pltfvndcvs ftttvsarfw lmdcrqvsea tkfatelyae
     1321 amhvpfmakf vvfakrqdpm eaqlrvfcmt ddkedktles qehftevaks rdvevlekks
     1381 qylefggnlv pvtksgeqlq lqfqafrenr lpfavrvkdp hqeplgrlaf mrqpraarat
     1441 eppqaplcnl nialpeyeas qnlselvtle kkygfveetg lakpelihra dlrlsdiare
     1501 lgsdwpalaa qldvpeqdva sirtecpsdl aqqallmlrl wmkraggrat gnnlekglrm
     1561 inredivnkc mfnvelvtdd vekavakvhl dqsgfdtfre elgtpkdtsl krdasldvsy
     1621 deqdlmkeae saeetssetg svhekhtgse akgtskaipg vrkgspisaa igrivrgssk
     1681 ekaaprmave etfeeepvlk idsaevtqqa apeagptapp rdskkkskke kspsplgpdy
     1741 slprmrdyht perdftsttd tlivssiked vdaskelatd gkvkkekkke kkekspsppg
     1801 pdytlprlrd tytppsdvta agyaastlpg qgitleqgiv keplerkske rslspgvvkt
     1861 gengslgadp sverqvkqls qgpedtiyek vervvketpg kivemvhikr qrsskspegr
     1921 gspkdknkgl pssevvapdi sgesrsvpse tigaekkqkk hketsptkqg kkeakevsea
     1981 vdkdaelsas dgklpdsgfl skmaklpkkm fprsgkasas deepsslses spvakkkagk
     2041 lpksatlpka kpsktplyei ksvdvevdts kltskgvkgv pakdgeglet srdkepdsrf
     2101 fskmaripkk mfprssktss sedepssase nlpsskqrak kekeisskkk ekvvkvkpds
     2161 kklstsfeve vdrdtlpgag vdinakkpgl qeslesesrk ktdsmqisfp qsvkavgskg
     2221 epmeysldie gmkslqkepq lavvgefpgk pspdielslt sketapefsd karghieigt
     2281 pehgksskgg fmtkmaklpr kmfphgfkgd vsveepsvea esvhvsvpea qvklgtkdlk
     2341 kkgpdmpame ltaslpvgae gsvemsapdl pvsadgglis kvtqfpkmfs hdsqdgvtie
     2401 rppvvgegid vesslpdvql kvkgkafekp etkfdvtlpe rkvtpvlpdg aeghievnld
     2461 emgksyddgf mskmaklpkk mfshgledei avqepsitqh dihvdipklk deleiratlp
     2521 ekpqvdfdva ipgkqvsldl pegveskiev sapdvgkptd ggfmskvvql pkkmfphgsk
     2581 gtvpleeape avgdihakvp eahlkvglkg lekpdfegdi avpgkkgavs lpegtegkie
     2641 vstpetgkps dkgfmskmak lpkkmfphgs kgsvsveevp evdrsvkvkv pqgkipeahl
     2701 kvdikdpekh kdvdialsee kvdidlpegi dknievtmpe vskpseggfi skmakfpkkm
     2761 fphgakgsvs veeapgidvd vkmpetrism dvkglekpkg evdislpkek vdidvpkgfd
     2821 gklemsapev gkpseggfms kmakfpkkmf phgakgsvsv eeapgidvdv kvpeahismd
     2881 vkglekprge vdislpkekv didvpkgfdg klemsapevg kpseggfmsk makfpkkmfp
     2941 hgakgslsve etpgidvdvk mpeadlsmdv kglekpkael diampkgkid idmpegvdgk
     3001 leasapdigk psdggfiskm tkfpkkmfph gakgtvsvea pelgdvqvnm peahlgvdik
     3061 gpekpkgeld vtlpkgkvdi dlpegiegkv eisapeigkp dggfmskmak lpkkmfphga
     3121 kggvsvegsp elgdvevkmp eahlgvdikg pekpkgeldv tlpkgkvdid lpegiegkve
     3181 isapeigkpd ggfmskmakl pkkmfphgak ggvsvegspe lgdvevkmpe ahlgvdikgp
     3241 ekpkgeldvt lpkgkvdidl pegiegkvei sapeigkpdg gfmskmaklp kkmfphgakg
     3301 gvsvegspel gdvevkmpea hlgvdikgpe kpkgeldvtl pkgkvdidlp egiegkveis
     3361 apeigkpdgg fmskmaklpk kmfshgakgg vsvegspelg dvqvkmpeah lgvdikgpek
     3421 pkgeldvtlp kgkvdidlpe giegkveiss peigkpdggf mskmaklpkk mfphgakggv
     3481 svegapelgd vevkmpeahl gvdikgpekp kgeldvtlpk gkvdidlpeg iegkveisap
     3541 eigkpdggfm skmaklpkkm fphgakggvs vegapelgdv evkmpeahlg vdikgpekpk
     3601 geldvtlpkg kvdidlpegf egkveisape igkpdggfms kmaklpkkmf phgtkggvsv
     3661 egapelgdve vkmpeahlgv dikgpekpkg eldvtlpkgk vdidlpegie gkveisspei
     3721 gkpdggfmsk maklpkkmfp hgakggvsve gapelgdvev kmpeahlgvd ikgpekpkge
     3781 ldvtlpkgkv didlpegieg kveisapeig kpdggfmskm aklpkkmfph gakggvsveg
     3841 spelgdvevk mpeahlgvdi kgpekpkgel dvtlpkgkvd idlpegiegk leisapeigk
     3901 pdggfmskma kfpkkmfphg akggvsvega pelgdvqvkm peahlgvdik gpekpkgeld
     3961 vtlpkgkvdi dlpegiegkv eisapeigkp dggfmskmak fpkkmfphga kggvsvegap
     4021 elgdvqvkmp eahlgvdieg pekpkgeldv tlpkgkvdid lpegiegkve isapeigkpd
     4081 ggfmskmakf pkkmfphgtk ggvsvegape lgdvevkmpe ahlgvdikgp ekpkgeldvt
     4141 lpkgkvdidl pegiegkvei sapeigkpdg gfmskmaklp qkmfphgtkg gvsvegapel
     4201 gdiqlkmpea hlgvdikgpe kpkgeldaml pkgkvdidlp egiegkveis apeigkpdgg
     4261 fmskmaklpk kmfshgakgs isvegspefg dvevkmpeah lggdikgpek pkgeldvalp
     4321 kgkvdidipe giegkveisa peigkpsdgg fvskmaklpk kmfphgtkgs vsiegapevg
     4381 dvqvkvpeaq lkmdikgtgk pefegeitwt gkkvavelpe giegtvemst pemgrpsdgg
     4441 fmskvaklpk kvfshgskgg vpfdeetgva gdievkmsea alkvdvkype kpesdfsale
     4501 kkididvpeg fkgkveistp elgkpsdggf lskmsklpkk vlphgskgei sagevsevsg
     4561 evpvelpear lkvgvkgper pdsdvdisvs dkkvtldvtk gfegkvevst pdlgkpsdkg
     4621 fiskvtkfpk kmfphgskgg vsvgevsevt gdvhvelpda hlkvgvkgpe rpdsdvdvsm
     4681 avkkitvdvp egfegklevs tpelarspdg slvskaaklp kklvpsgens lssveepsls
     4741 ahvdvpgsdt tfkvdaklpa mpvtdvdisl sakessadip vgvagkveig tpdagkpsdk
     4801 gfmskmakip kkmfhhgpks sgstdehstv aetvddkalg fevqlqkdvk iptkpeadvs
     4861 vsfhekevtv qlpegregtv elstpglvge hvpdkssqga dvllpgkelp tvegqeiiek
     4921 pggvieviev tahelskpls sgllskvski pkkifprgsk skssdedsps pgkrelketp
     4981 ggivevvevt khdvaihpes thvkqvgtev llepatsetk psgaeglppt hitreikevp
     5041 ggivevvrvt khivgklped kgigepsvrt tdaslpweae lakdpsavik revketpegh
     5101 vevvqvirre asdvpfgsdf pgdlesdgtv srriettvvd gkptvirrlv tttsdgirqv
     5161 tehieqgaed agsaasslfa kmgefakgls sddpsavttr evkqlpggav evvsvtkrss
     5221 sspeteitsq eqlqsstsgs mhfaesavgk pmeakttiir reisgpetiv rtttsvvtsa
     5281 tapgsskpel sadvldkmfg amekprvddr lrdvltaegg yvakrevtpe eaellaqlfp
     5341 eqstgvavyp paglvpeaai ipeyrcmtta egahhdpesk ekvllklgra lsqddldaps
     5401 ptqgeaamtv aglgeavdts dqveepavek lelarreskr ysqefeqdep lpkamgfktk
     5461 pseetvvsst qvkgvhdngt vkhtvttvtk ttiiqeveep agaprpgese twasdldtsq
     5521 vsvsslpede rteptlsssa ttatadaeal sgplartqve ekewtetdpd sgevrhiisk
     5581 tthttvtskk psegaprgdt tsttvshssk dghhgstpsa psecdeaesl tffqkkeffe
     5641 klseqsppke tssarlgstp sldtaltrpa vtlkprprse sltsqgeiie esivfaerlr
     5701 lfqngaqasp lpsasipqvd lvdpapkmeq klqgmaeigd slqqvpsavl eglqsteeks
     5761 dtrllvhtsd adkeafdvrs vqvaedfgdk qfshmrvrmp apeetvskts icssdlpgdk
     5821 psplrdaykg slsasrqess qagkpplpsa lggdsslclv heattdltls kagppnqtnk
     5881 dittfdrqpv reqsptlrss qsqmlasglq mgivagsgil qegikaspqs ailetfsssa
     5941 temtglespe irglpseevh agsaeeakgi dagrigavla aapvailpvr diisgkqlem
     6001 ldiaqvsdep nifegcvelp sqaesqtcpk ektimkteya diqhklssaf geslapvesk
     6061 sgvtqgvpke vlealdrssq eliasklded serdtnilsa tqgsiphdvf pdhiestldn
     6121 saavpmrgay stpsstavcl tperleiqat revaeerqek pevlvaleay rkstlrrsge
     6181 dsydrnleef itvleeyepl pagqlsdwkr aspafepsic hnseitsklp pledkkelet
     6241 vgsildpsen vsellgnhaq sqmlcedtgl ltdelgrtag hyvistaesd rgeclptlse
     6301 qapgkereec lnydsalgvr stsglessll eesheersld larsedeqps lvedsmnvpt
     6361 essvidyqvv ghsgvvkdaq tqpkdsdfdl heisrtgdkt dlplscgddg kksaefsktv
     6421 sgiaadmvik dtdsehlsqd mlhptastvl cegrsdvpys sdedqhkels rykevelvip
     6481 flrgtsssle devedggsfs sgdlqaekia eflttdpdtl nkvvhfpqif qdydtsahqe
     6541 pfvptetemn vekhvgfvld emaacstpcv plksdrqtei eeasrivesv vhiasslves
     6601 lhevnhetai kedicdsala yaagsplers evsetlsqag ppstiekdqr espfgvkemy
     6661 qvlkesrgse driqysvtsf gdplfadegd edqerrrrgs ptaeslddqa qiekcshevm
     6721 mqlldgfsqs dvelaevrte deapdieddv pspeagshee ksvpssqdns gdlqpqlste
     6781 veigsqasga paplseldye lcpdadqlrt sgdeavatrv ppleperdea vaesshapsa
     6841 aadpaasagr naggrerspl leasppeeta ycpeqdispa qeypeyltpd mqvrvgvspt
     6901 eaqpakekpv rysessvess sevgspcpps qggtamafen vafagedvid atsspieess
     6961 spagsrspye vvrdahaeea aqtalveaek slrhavqeev rarpqslvte esseqqehqi
     7021 qpsifneafd hspeesapek kqlsegllgv glltvsdtta rtpesrrgds dedeeewadh
     7081 vmskirgrpi pptppaspra vqsavevqge pweapvveqe rvnvesardm saerdagvdv
     7141 mgdrsatqpq lqefrrtadg krpqlehips eisdedlver eqspthitat crgdrkdsss
     7201 drnsspdsdg pkasgshrrs asgvgaiale avhsqdsggn ekhshesrdk gaqeddkite
     7261 epvdtmfvie eeapadsnrs esfcddader aartagglsp slesppqkkd lddgsssgkp
     7321 daaspvvlrs kskrtdkisd krssrdldtg hsgsetdhsh yysfeltsds gktpgasrpt
     7381 ssefdvnils gqasseyetc atsqdgevsg syataaassh dtsfatarss lsgssrssay
     7441 svdsessghl gsmevsease tivasaheel dsdiesersk svlrepydge ipesvirggv
     7501 ehpflpswqp alkdssptlh gsagarppta egvfspfqst vpsepgrscv gwpssslsta
     7561 esvrspskpe vlngvsaple gqrrlrhndd fmassheeeq lsvsessatq sehtwqtsve
     7621 tmvdaveaqr fscdserhhr qstmqnsvsl pfdeavvaed shreadddra tppfqevngp
     7681 veveyvpeyd dltartlrpv rqqnapdgqt eaqgdvalle asagdastsr eqheeenste
     7741 lreegfyaet fqgdlsseev esaanealll dksacsfyey chakdeyevc ntrpavesas
     7801 lkeaferpat pepvdeiddq edegnlaqgs pnlhgkdyea fghasaeesv pcsdkthiaq
     7861 elsendqykv fsdsgnqssl hdetrsksvs ssleramtdd vrlytskced yilqmhqtyk
     7921 ygvglpktsd ggylpeegmd venvkllesr lqegnggqqd fpydlsyqqe yggdgrvehg
     7981 dlythqevee egyddersrt rgrrrlspis dergstpdyd tlagrktfgk gsekddasts
     8041 slmeferlea emasgtksss vgssdslsgr qhqgrsgags eqdsvsvnsl teferlekei
     8101 veaegvdarl rgevaaklde ideghesqas dpghetavyd esgatlllca sadvneepdg
     8161 ttddaqlgdi eaaqgqevaw srterhfltt rtesghsves vdrqssasta tqfdadslre
     8221 reiddsesnf gpdtpgwmqk ecvdldslhd slhdsehhds lqevpskdkd slqlrepsad
     8281 adslqedhne lqdslyetrd idwdsfrade tsglddtsrs whqfpsqirt telsptlsrp
     8341 assplvvpvv veridtltwv dqdpakacvs dgeslgsrqq ehprndalvs stdslepsss
     8401 anthatyqcd tdsmmsslns aeestmvtst dtlehetlcl asqgkslsle gglfsggdvt
     8461 eeqntrtple ggvsvddmee rfrecllrsa asptneeqve erqtmdekgn livtrtvkrh
     8521 vtseprlhtq tfmgpdaeqr srefvasfaa mepsaerdey egfdaagntv rvtqhvvvrp
     8581 tiktvtftgp darlqmreym rslaggqhss aentptsdsg vgacsslasp pplmpdppaa
     8641 apssacpglq rlsggdqwme aesalddcsp pvtatrvitt rttrtmgpdg reqlvtttte
     8701 esggeepear lrrsmqgvld sfmaepptpp hldeee
//
LOCUS       XP_077503792            8735 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503792
VERSION     XP_077503792.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647666.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8735
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..8735
                     /product="uncharacterized protein LOC144114148 isoform X2"
                     /calculated_mol_wt=938432
     Region          59..90
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          73..390
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(92,94,98..99,102..104,106..107,111,114,123,125,127,
                     131..132,135..137,139..140,144,147,156,158,160,164..165,
                     168..170,172..173,177,180)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          92..123
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          125..156
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          158..183
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          191..216
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          224..251
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(253,255,259..260,263..265,267..268,272,275,284,286,
                     288,292..293,296..298,300..301,305,308,317,319,321,
                     325..326,329..331,333..334,338,341)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          253..284
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          286..317
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          319..349
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          333..621
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          352..416
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          418..449
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(451,453,457..458,461..463,465..466,470,473,482,484,
                     486,490..491,494..496,498..499,503,506,515,517,519,
                     523..524,527..529,531..532,536,539)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          451..482
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          484..515
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          499..777
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          517..546
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          550..575
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          583..614
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          616..649
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          650..680
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          682..712
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(715,717,721..722,725..727,729..730,734,737,746,748,
                     750,754..755,758..760,762..763,767,770,779,781,783,
                     787..788,791..793,795..796,800,803)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          715..746
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          748..779
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          753..834
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          781..811
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          999..1101
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1324..1456
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1488..1571
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <1884..2135
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          <4498..4758
                     /region_name="YbbR"
                     /note="Cyclic di-AMP synthase regulator CdaR, YbbR domain
                     [Signal transduction mechanisms]; COG4856"
                     /db_xref="CDD:443884"
     Region          <7163..7476
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..8735
                     /gene="LOC144114148"
                     /coded_by="XM_077647666.1:883..27090"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 mgpgeptirq sreswigcli rllasqndqc dpsvylflrw ltllllravl sqmmppatdg
       61 nasflraara gnlekvldyl kgsidintsn anglnalhla akeghvnvvs ellkrganvn
      121 attkkgnsal hiaslagqee vvkllvqkqa nvnvqsqngf tplymaaqen hdgvvrflla
      181 nganqslate dgftplaval qqghdkvvav llendargkv rlpalhiask kddckaaall
      241 lhsehnpdvt sksgftplhi aahygnsnia sllldkgadv nfpakhqitp lhvaakwgks
      301 nmvklllekg akidastrdg ltplhcaars ghdqvveqll eknapitakt knglaplhma
      361 sqgdhvdsar illyhkapvd dvtvdyltal hvaahcghvg vakllldrra dpnaralngf
      421 tplhiackkn rikvvelllk hgasieatte sgltplhvas fmgcmniviy liqhganpdi
      481 ptvrgetplh laaranqtdi irillrngah vdakarelqt alhiasrlgn admvglllqh
      541 gaavdaptkd aytplhvaar egqdevaall ldhgaalaap tkkgftplhl aakygnlkva
      601 qlllqkdapv daqgkngvtp lhvaahydhv nvallllekg asphsaarng ytplhvaark
      661 dqmdiassll eygakpgaes ragftplhla aqeghadlaa llvehgadcd akakngltpm
      721 hlcaqedrve vatilakhga sldpttkagy tplhvachfg qtnmirfllr qganvnatts
      781 hgytplhqaa qqghtliinl llehraapna vtnqgqtala iaqrlgyisv vetlkvvtet
      841 ivttttttvt eekyrvvape tmhetfmsds edeaaednml gdqsfrylta demkslgdds
      901 lpidvtrder itesihitre pghpapltqe eerlsptqah tteaifvgny apdnvdlsrt
      961 pihagsllsw dgdscssayv lsptkrmwre ssklkwktfl vsflvdargg amrgcrhsgv
     1021 rviipprkaq mpmritcryl rkeklphppp llegeacasr ilevgpagak flgpvilevp
     1081 hfaslrgker eisilrsdng etwkehtlea seeavqevln esfegeelsa ledlqtnriv
     1141 rilttdfpqy favvsrtrqe vhavgpeggl lsstvvpqvq aifpegaltk kirvglqaqp
     1201 ipaelvtkll ghrvavspiv tveprrrkfh kpitltipvp qaatkgminq yagdaptlrl
     1261 lcsitggtnk aqwedvtgst pltfvndcvs ftttvsarfw lmdcrqvsea tkfatelyae
     1321 amhvpfmakf vvfakrqdpm eaqlrvfcmt ddkedktles qehftevaks rdvevlekks
     1381 qylefggnlv pvtksgeqlq lqfqafrenr lpfavrvkdp hqeplgrlaf mrqpraarat
     1441 eppqaplcnl nialpeyeas qnlselvtle kkygfveetg lakpelihra dlrlsdiare
     1501 lgsdwpalaa qldvpeqdva sirtecpsdl aqqallmlrl wmkraggrat gnnlekglrm
     1561 inredivnkc mfnvelvtdd vekavakvhl dqsgfdtfre elgtpkdtsl krdasldvsy
     1621 deqdlmkaes aeetssetgs vhekhtgsea kgtskaipgv rkgspisaai grivrgsske
     1681 kaaprmavee tfeeepvlki dsaevtqqaa peagptappr dskkkskkek spsplgpdys
     1741 lprmrdyhtp erdftsttdt livssikedv daskelatdg kvkkekkkek kekspsppgp
     1801 dytlprlrdt ytppsdvtaa gyaastlpgq gitleqgivk eplerksker slspgvvktg
     1861 engslgadps verqvkqlsq gpedtiyekv ervvketpgk ivemvhikrq rsskspegrg
     1921 spkdknkglp ssevvapdis gesrsvpset igaekkqkkh ketsptkqgk keakevseav
     1981 dkdaelsasd gklpdsgfls kmaklpkkmf prsgkasasd eepsslsess pvakkkagkl
     2041 pksatlpkak psktplyeik svdvevdtsk ltskgvkgvp akdgeglets rdkepdsrff
     2101 skmaripkkm fprssktsss edepssasen lpsskqrakk ekeisskkke kvvkvkpdsk
     2161 klstsfevev drdtlpgagv dinakkpglq eslesesrkk tdsmqisfpq svkavgskge
     2221 pmeysldieg mkslqkepql avvgefpgkp spdielslts ketapefsdk arghieigtp
     2281 ehgksskggf mtkmaklprk mfphgfkgdv sveepsveae svhvsvpeaq vklgtkdlkk
     2341 kgpdmpamel taslpvgaeg svemsapdlp vsadgglisk vtqfpkmfsh dsqdgvtier
     2401 ppvvgegidv esslpdvqlk vkgkafekpe tkfdvtlper kvtpvlpdga eghievnlde
     2461 mgksyddgfm skmaklpkkm fshgledeia vqepsitqhd ihvdipklkd eleiratlpe
     2521 kpqvdfdvai pgkqvsldlp egveskievs apdvgkptdg gfmskvvqlp kkmfphgskg
     2581 tvpleeapea vgdihakvpe ahlkvglkgl ekpdfegdia vpgkkgavsl pegtegkiev
     2641 stpetgkpsd kgfmskmakl pkkmfphgsk gsvsveevpe vdrsvkvkvp qgkipeahlk
     2701 vdikdpekhk dvdialseek vdidlpegid knievtmpev skpseggfis kmakfpkkmf
     2761 phgakgsvsv eeapgidvdv kmpetrismd vkglekpkge vdislpkekv didvpkgfdg
     2821 klemsapevg kpseggfmsk makfpkkmfp hgakgsvsve eapgidvdvk vpeahismdv
     2881 kglekprgev dislpkekvd idvpkgfdgk lemsapevgk pseggfmskm akfpkkmfph
     2941 gakgslsvee tpgidvdvkm peadlsmdvk glekpkaeld iampkgkidi dmpegvdgkl
     3001 easapdigkp sdggfiskmt kfpkkmfphg akgtvsveap elgdvqvnmp eahlgvdikg
     3061 pekpkgeldv tlpkgkvdid lpegiegkve isapeigkpd ggfmskmakl pkkmfphgak
     3121 ggvsvegspe lgdvevkmpe ahlgvdikgp ekpkgeldvt lpkgkvdidl pegiegkvei
     3181 sapeigkpdg gfmskmaklp kkmfphgakg gvsvegspel gdvevkmpea hlgvdikgpe
     3241 kpkgeldvtl pkgkvdidlp egiegkveis apeigkpdgg fmskmaklpk kmfphgakgg
     3301 vsvegspelg dvevkmpeah lgvdikgpek pkgeldvtlp kgkvdidlpe giegkveisa
     3361 peigkpdggf mskmaklpkk mfshgakggv svegspelgd vqvkmpeahl gvdikgpekp
     3421 kgeldvtlpk gkvdidlpeg iegkveissp eigkpdggfm skmaklpkkm fphgakggvs
     3481 vegapelgdv evkmpeahlg vdikgpekpk geldvtlpkg kvdidlpegi egkveisape
     3541 igkpdggfms kmaklpkkmf phgakggvsv egapelgdve vkmpeahlgv dikgpekpkg
     3601 eldvtlpkgk vdidlpegfe gkveisapei gkpdggfmsk maklpkkmfp hgtkggvsve
     3661 gapelgdvev kmpeahlgvd ikgpekpkge ldvtlpkgkv didlpegieg kveisspeig
     3721 kpdggfmskm aklpkkmfph gakggvsveg apelgdvevk mpeahlgvdi kgpekpkgel
     3781 dvtlpkgkvd idlpegiegk veisapeigk pdggfmskma klpkkmfphg akggvsvegs
     3841 pelgdvevkm peahlgvdik gpekpkgeld vtlpkgkvdi dlpegiegkl eisapeigkp
     3901 dggfmskmak fpkkmfphga kggvsvegap elgdvqvkmp eahlgvdikg pekpkgeldv
     3961 tlpkgkvdid lpegiegkve isapeigkpd ggfmskmakf pkkmfphgak ggvsvegape
     4021 lgdvqvkmpe ahlgvdiegp ekpkgeldvt lpkgkvdidl pegiegkvei sapeigkpdg
     4081 gfmskmakfp kkmfphgtkg gvsvegapel gdvevkmpea hlgvdikgpe kpkgeldvtl
     4141 pkgkvdidlp egiegkveis apeigkpdgg fmskmaklpq kmfphgtkgg vsvegapelg
     4201 diqlkmpeah lgvdikgpek pkgeldamlp kgkvdidlpe giegkveisa peigkpdggf
     4261 mskmaklpkk mfshgakgsi svegspefgd vevkmpeahl ggdikgpekp kgeldvalpk
     4321 gkvdidipeg iegkveisap eigkpsdggf vskmaklpkk mfphgtkgsv siegapevgd
     4381 vqvkvpeaql kmdikgtgkp efegeitwtg kkvavelpeg iegtvemstp emgrpsdggf
     4441 mskvaklpkk vfshgskggv pfdeetgvag dievkmseaa lkvdvkypek pesdfsalek
     4501 kididvpegf kgkveistpe lgkpsdggfl skmsklpkkv lphgskgeis agevsevsge
     4561 vpvelpearl kvgvkgperp dsdvdisvsd kkvtldvtkg fegkvevstp dlgkpsdkgf
     4621 iskvtkfpkk mfphgskggv svgevsevtg dvhvelpdah lkvgvkgper pdsdvdvsma
     4681 vkkitvdvpe gfegklevst pelarspdgs lvskaaklpk klvpsgensl ssveepslsa
     4741 hvdvpgsdtt fkvdaklpam pvtdvdisls akessadipv gvagkveigt pdagkpsdkg
     4801 fmskmakipk kmfhhgpkss gstdehstva etvddkalgf evqlqkdvki ptkpeadvsv
     4861 sfhekevtvq lpegregtve lstpglvgeh vpdkssqgad vllpgkelpt vegqeiiekp
     4921 ggvievievt ahelskplss gllskvskip kkifprgsks kssdedspsp gkrelketpg
     4981 givevvevtk hdvaihpest hvkqvgtevl lepatsetkp sgaeglppth itreikevpg
     5041 givevvrvtk hivgklpedk gigepsvrtt daslpweael akdpsavikr evketpeghv
     5101 evvqvirrea sdvpfgsdfp gdlesdgtvs rriettvvdg kptvirrlvt ttsdgirqvt
     5161 ehieqgaeda gsaasslfak mgefakglss ddpsavttre vkqlpggave vvsvtkrsss
     5221 speteitsqe qlqsstsgsm hfaesavgkp meakttiirr eisgpetivr tttsvvtsat
     5281 apgsskpels advldkmfga mekprvddrl rdvltaeggy vakrevtpee aellaqlfpe
     5341 qstgvavypp aglvpeaaii peyrcmttae gahhdpeske kvllklgral sqddldapsp
     5401 tqgeaamtva glgeavdtsd qveepavekl elarreskry sqefeqdepl pkamgfktkp
     5461 seetvvsstq vkgvhdngtv khtvttvtkt tiiqeveepa gaprpgeset wasdldtsqv
     5521 svsslpeder teptlsssat tatadaeals gplartqvee kewtetdpds gevrhiiskt
     5581 thttvtskkp segaprgdtt sttvshsskd ghhgstpsap secdeaeslt ffqkkeffek
     5641 lseqsppket ssarlgstps ldtaltrpav tlkprprses ltsqgeiiee sivfaerlrl
     5701 fqngaqaspl psasipqvdl vdpapkmeqk lqgmaeigds lqqvpsavle glqsteeksd
     5761 trllvhtsda dkeafdvrsv qvaedfgdkq fshmrvrmpa peetvsktsi cssdlpgdkp
     5821 splrdaykgs lsasrqessq agkpplpsal ggdsslclvh eattdltlsk agppnqtnkd
     5881 ittfdrqpvr eqsptlrssq sqmlasglqm givagsgilq egikaspqsa iletfsssat
     5941 emtglespei rglpseevha gsaeeakgid agrigavlaa apvailpvrd iisgkqleml
     6001 diaqvsdepn ifegcvelps qaesqtcpke ktimkteyad iqhklssafg eslapvesks
     6061 gvtqgvpkev lealdrssqe liaskldeds erdtnilsat qgsiphdvfp dhiestldns
     6121 aavpmrgays tpsstavclt perleiqatr evaeerqekp evlvaleayr kstlrrsged
     6181 sydrnleefi tvleeyeplp agqlsdwkra spafepsich nseitsklpp ledkkeletv
     6241 gsildpsenv sellgnhaqs qmlcedtgll tdelgrtagh yvistaesdr geclptlseq
     6301 apgkereecl nydsalgvrs tsglesslle esheersldl arsedeqpsl vedsmnvpte
     6361 ssvidyqvvg hsgvvkdaqt qpkdsdfdlh eisrtgdktd lplscgddgk ksaefsktvs
     6421 giaadmvikd tdsehlsqdm lhptastvlc egrsdvpyss dedqhkelsr ykevelvipf
     6481 lrgtsssled evedggsfss gdlqaekiae flttdpdtln kvvhfpqifq dydtsahqep
     6541 fvptetemnv ekhvgfvlde maacstpcvp lksdrqteie easrivesvv hiasslvesl
     6601 hevnhetaik edicdsalay aagsplerse vsetlsqagp pstiekdqre spfgvkemyq
     6661 vlkesrgsed riqysvtsfg dplfadegde dqerrrrgsp taeslddqaq iekcshevmm
     6721 qlldgfsqsd velaevrted eapdieddvp speagsheek svpssqdnsg dlqpqlstev
     6781 eigsqasgap aplseldyel cpdadqlrts gdeavatrvp pleperdeav aesshapsaa
     6841 adpaasagrn aggrerspll easppeetay cpeqdispaq eypeyltpdm qvrvgvspte
     6901 aqpakekpvr ysessvesss evgspcppsq ggtamafenv afagedvida tsspieesss
     6961 pagsrspyev vrdahaeeaa qtalveaeks lrhavqeevr arpqslvtee sseqqehqiq
     7021 psifneafdh speesapekk qlsegllgvg lltvsdttar tpesrrgdsd edeeewadhv
     7081 mskirgrpip ptppasprav qsavevqgep weapvveqer vnvesardms aerdagvdvm
     7141 gdrsatqpql qefrrtadgk rpqlehipse isdedlvere qspthitatc rgdrkdsssd
     7201 rnsspdsdgp kasgshrrsa sgvgaialea vhsqdsggne khshesrdkg aqeddkitee
     7261 pvdtmfviee eapadsnrse sfcddadera artagglsps lesppqkkdl ddgsssgkpd
     7321 aaspvvlrsk skrtdkisdk rssrdldtgh sgsetdhshy ysfeltsdsg ktpgasrpts
     7381 sefdvnilsg qasseyetca tsqdgevsgs yataaasshd tsfatarssl sgssrssays
     7441 vdsessghlg smevseaset ivasaheeld sdiesersks vlrepydgei pesvirggve
     7501 hpflpswqpa lkdssptlhg sagarpptae gvfspfqstv psepgrscvg wpssslstae
     7561 svrspskpev lngvsapleg qrrlrhnddf massheeeql svsessatqs ehtwqtsvet
     7621 mvdaveaqrf scdserhhrq stmqnsvslp fdeavvaeds hreadddrat ppfqevngpv
     7681 eveyvpeydd ltartlrpvr qqnapdgqte aqgdvallea sagdastsre qheeenstel
     7741 reegfyaetf qgdlsseeve saaneallld ksacsfyeyc hakdeyevcn trpavesasl
     7801 keaferpatp epvdeiddqe degnlaqgsp nlhgkdyeaf ghasaeesvp csdkthiaqe
     7861 lsendqykvf sdsgnqsslh detrsksvss sleramtddv rlytskcedy ilqmhqtyky
     7921 gvglpktsdg gylpeegmdv envkllesrl qegnggqqdf pydlsyqqey ggdgrvehgd
     7981 lythqeveee gyddersrtr grrrlspisd ergstpdydt lagrktfgkg sekddastss
     8041 lmeferleae masgtksssv gssdslsgrq hqgrsgagse qdsvsvnslt eferlekeiv
     8101 eaegvdarlr gevaakldei deghesqasd pghetavyde sgatlllcas advneepdgt
     8161 tddaqlgdie aaqgqevaws rterhflttr tesghsvesv drqssastat qfdadslrer
     8221 eiddsesnfg pdtpgwmqke cvdldslhds lhdsehhdsl qevpskdkds lqlrepsada
     8281 dslqedhnel qdslyetrdi dwdsfradet sglddtsrsw hqfpsqirtt elsptlsrpa
     8341 ssplvvpvvv eridtltwvd qdpakacvsd geslgsrqqe hprndalvss tdslepsssa
     8401 nthatyqcdt dsmmsslnsa eestmvtstd tlehetlcla sqgkslsleg glfsggdvte
     8461 eqntrtpleg gvsvddmeer frecllrsaa sptneeqvee rqtmdekgnl ivtrtvkrhv
     8521 tseprlhtqt fmgpdaeqrs refvasfaam epsaerdeye gfdaagntvr vtqhvvvrpt
     8581 iktvtftgpd arlqmreymr slaggqhssa entptsdsgv gacsslaspp plmpdppaaa
     8641 pssacpglqr lsggdqwmea esalddcspp vtatrvittr ttrtmgpdgr eqlvttttee
     8701 sggeepearl rrsmqgvlds fmaepptpph ldeee
//
LOCUS       XP_077503793            8735 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503793
VERSION     XP_077503793.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647667.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8735
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..8735
                     /product="uncharacterized protein LOC144114148 isoform X3"
                     /calculated_mol_wt=938474
     Region          59..90
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          73..390
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(92,94,98..99,102..104,106..107,111,114,123,125,127,
                     131..132,135..137,139..140,144,147,156,158,160,164..165,
                     168..170,172..173,177,180)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          92..123
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          125..156
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          158..183
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          191..216
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          224..251
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(253,255,259..260,263..265,267..268,272,275,284,286,
                     288,292..293,296..298,300..301,305,308,317,319,321,
                     325..326,329..331,333..334,338,341)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          253..284
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          286..317
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          319..349
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          333..621
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          352..416
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          418..449
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(451,453,457..458,461..463,465..466,470,473,482,484,
                     486,490..491,494..496,498..499,503,506,515,517,519,
                     523..524,527..529,531..532,536,539)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          451..482
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          484..515
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          499..777
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          517..546
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          550..575
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          583..614
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          616..649
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          650..680
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          682..712
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(715,717,721..722,725..727,729..730,734,737,746,748,
                     750,754..755,758..760,762..763,767,770,779,781,783,
                     787..788,791..793,795..796,800,803)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          715..746
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          748..779
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          753..834
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          781..811
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          998..1100
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1323..1455
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1487..1570
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <1884..2135
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          <4498..4758
                     /region_name="YbbR"
                     /note="Cyclic di-AMP synthase regulator CdaR, YbbR domain
                     [Signal transduction mechanisms]; COG4856"
                     /db_xref="CDD:443884"
     Region          <7163..7476
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..8735
                     /gene="LOC144114148"
                     /coded_by="XM_077647667.1:884..27091"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 mgpgeptirq sreswigcli rllasqndqc dpsvylflrw ltllllravl sqmmppatdg
       61 nasflraara gnlekvldyl kgsidintsn anglnalhla akeghvnvvs ellkrganvn
      121 attkkgnsal hiaslagqee vvkllvqkqa nvnvqsqngf tplymaaqen hdgvvrflla
      181 nganqslate dgftplaval qqghdkvvav llendargkv rlpalhiask kddckaaall
      241 lhsehnpdvt sksgftplhi aahygnsnia sllldkgadv nfpakhqitp lhvaakwgks
      301 nmvklllekg akidastrdg ltplhcaars ghdqvveqll eknapitakt knglaplhma
      361 sqgdhvdsar illyhkapvd dvtvdyltal hvaahcghvg vakllldrra dpnaralngf
      421 tplhiackkn rikvvelllk hgasieatte sgltplhvas fmgcmniviy liqhganpdi
      481 ptvrgetplh laaranqtdi irillrngah vdakarelqt alhiasrlgn admvglllqh
      541 gaavdaptkd aytplhvaar egqdevaall ldhgaalaap tkkgftplhl aakygnlkva
      601 qlllqkdapv daqgkngvtp lhvaahydhv nvallllekg asphsaarng ytplhvaark
      661 dqmdiassll eygakpgaes ragftplhla aqeghadlaa llvehgadcd akakngltpm
      721 hlcaqedrve vatilakhga sldpttkagy tplhvachfg qtnmirfllr qganvnatts
      781 hgytplhqaa qqghtliinl llehraapna vtnqgqtala iaqrlgyisv vetlkvvtet
      841 ivttttttvt eekyrvvape tmhetfmsds edeaaednml gdqsfrylta demkslgdds
      901 lpidvtrder itesihitre pghpapltqe eerlsptqah tteaifvgny apdnvdlsrt
      961 pihagsllsw dgdscssayv lsptkrmwre sklkwktflv sflvdargga mrgcrhsgvr
     1021 viipprkaqm pmritcrylr keklphpppl legeacasri levgpagakf lgpvilevph
     1081 faslrgkere isilrsdnge twkehtleas eeavqevlne sfegeelsal edlqtnrivr
     1141 ilttdfpqyf avvsrtrqev havgpeggll sstvvpqvqa ifpegaltkk irvglqaqpi
     1201 paelvtkllg hrvavspivt veprrrkfhk pitltipvpq aatkgminqy agdaptlrll
     1261 csitggtnka qwedvtgstp ltfvndcvsf tttvsarfwl mdcrqvseat kfatelyaea
     1321 mhvpfmakfv vfakrqdpme aqlrvfcmtd dkedktlesq ehftevaksr dvevlekksq
     1381 ylefggnlvp vtksgeqlql qfqafrenrl pfavrvkdph qeplgrlafm rqpraarate
     1441 ppqaplcnln ialpeyeasq nlselvtlek kygfveetgl akpelihrad lrlsdiarel
     1501 gsdwpalaaq ldvpeqdvas irtecpsdla qqallmlrlw mkraggratg nnlekglrmi
     1561 nredivnkcm fnvelvtddv ekavakvhld qsgfdtfree lgtpkdtslk rdasldvsyd
     1621 eqdlmkeaes aeetssetgs vhekhtgsea kgtskaipgv rkgspisaai grivrgsske
     1681 kaaprmavee tfeeepvlki dsaevtqqaa peagptappr dskkkskkek spsplgpdys
     1741 lprmrdyhtp erdftsttdt livssikedv daskelatdg kvkkekkkek kekspsppgp
     1801 dytlprlrdt ytppsdvtaa gyaastlpgq gitleqgivk eplerksker slspgvvktg
     1861 engslgadps verqvkqlsq gpedtiyekv ervvketpgk ivemvhikrq rsskspegrg
     1921 spkdknkglp ssevvapdis gesrsvpset igaekkqkkh ketsptkqgk keakevseav
     1981 dkdaelsasd gklpdsgfls kmaklpkkmf prsgkasasd eepsslsess pvakkkagkl
     2041 pksatlpkak psktplyeik svdvevdtsk ltskgvkgvp akdgeglets rdkepdsrff
     2101 skmaripkkm fprssktsss edepssasen lpsskqrakk ekeisskkke kvvkvkpdsk
     2161 klstsfevev drdtlpgagv dinakkpglq eslesesrkk tdsmqisfpq svkavgskge
     2221 pmeysldieg mkslqkepql avvgefpgkp spdielslts ketapefsdk arghieigtp
     2281 ehgksskggf mtkmaklprk mfphgfkgdv sveepsveae svhvsvpeaq vklgtkdlkk
     2341 kgpdmpamel taslpvgaeg svemsapdlp vsadgglisk vtqfpkmfsh dsqdgvtier
     2401 ppvvgegidv esslpdvqlk vkgkafekpe tkfdvtlper kvtpvlpdga eghievnlde
     2461 mgksyddgfm skmaklpkkm fshgledeia vqepsitqhd ihvdipklkd eleiratlpe
     2521 kpqvdfdvai pgkqvsldlp egveskievs apdvgkptdg gfmskvvqlp kkmfphgskg
     2581 tvpleeapea vgdihakvpe ahlkvglkgl ekpdfegdia vpgkkgavsl pegtegkiev
     2641 stpetgkpsd kgfmskmakl pkkmfphgsk gsvsveevpe vdrsvkvkvp qgkipeahlk
     2701 vdikdpekhk dvdialseek vdidlpegid knievtmpev skpseggfis kmakfpkkmf
     2761 phgakgsvsv eeapgidvdv kmpetrismd vkglekpkge vdislpkekv didvpkgfdg
     2821 klemsapevg kpseggfmsk makfpkkmfp hgakgsvsve eapgidvdvk vpeahismdv
     2881 kglekprgev dislpkekvd idvpkgfdgk lemsapevgk pseggfmskm akfpkkmfph
     2941 gakgslsvee tpgidvdvkm peadlsmdvk glekpkaeld iampkgkidi dmpegvdgkl
     3001 easapdigkp sdggfiskmt kfpkkmfphg akgtvsveap elgdvqvnmp eahlgvdikg
     3061 pekpkgeldv tlpkgkvdid lpegiegkve isapeigkpd ggfmskmakl pkkmfphgak
     3121 ggvsvegspe lgdvevkmpe ahlgvdikgp ekpkgeldvt lpkgkvdidl pegiegkvei
     3181 sapeigkpdg gfmskmaklp kkmfphgakg gvsvegspel gdvevkmpea hlgvdikgpe
     3241 kpkgeldvtl pkgkvdidlp egiegkveis apeigkpdgg fmskmaklpk kmfphgakgg
     3301 vsvegspelg dvevkmpeah lgvdikgpek pkgeldvtlp kgkvdidlpe giegkveisa
     3361 peigkpdggf mskmaklpkk mfshgakggv svegspelgd vqvkmpeahl gvdikgpekp
     3421 kgeldvtlpk gkvdidlpeg iegkveissp eigkpdggfm skmaklpkkm fphgakggvs
     3481 vegapelgdv evkmpeahlg vdikgpekpk geldvtlpkg kvdidlpegi egkveisape
     3541 igkpdggfms kmaklpkkmf phgakggvsv egapelgdve vkmpeahlgv dikgpekpkg
     3601 eldvtlpkgk vdidlpegfe gkveisapei gkpdggfmsk maklpkkmfp hgtkggvsve
     3661 gapelgdvev kmpeahlgvd ikgpekpkge ldvtlpkgkv didlpegieg kveisspeig
     3721 kpdggfmskm aklpkkmfph gakggvsveg apelgdvevk mpeahlgvdi kgpekpkgel
     3781 dvtlpkgkvd idlpegiegk veisapeigk pdggfmskma klpkkmfphg akggvsvegs
     3841 pelgdvevkm peahlgvdik gpekpkgeld vtlpkgkvdi dlpegiegkl eisapeigkp
     3901 dggfmskmak fpkkmfphga kggvsvegap elgdvqvkmp eahlgvdikg pekpkgeldv
     3961 tlpkgkvdid lpegiegkve isapeigkpd ggfmskmakf pkkmfphgak ggvsvegape
     4021 lgdvqvkmpe ahlgvdiegp ekpkgeldvt lpkgkvdidl pegiegkvei sapeigkpdg
     4081 gfmskmakfp kkmfphgtkg gvsvegapel gdvevkmpea hlgvdikgpe kpkgeldvtl
     4141 pkgkvdidlp egiegkveis apeigkpdgg fmskmaklpq kmfphgtkgg vsvegapelg
     4201 diqlkmpeah lgvdikgpek pkgeldamlp kgkvdidlpe giegkveisa peigkpdggf
     4261 mskmaklpkk mfshgakgsi svegspefgd vevkmpeahl ggdikgpekp kgeldvalpk
     4321 gkvdidipeg iegkveisap eigkpsdggf vskmaklpkk mfphgtkgsv siegapevgd
     4381 vqvkvpeaql kmdikgtgkp efegeitwtg kkvavelpeg iegtvemstp emgrpsdggf
     4441 mskvaklpkk vfshgskggv pfdeetgvag dievkmseaa lkvdvkypek pesdfsalek
     4501 kididvpegf kgkveistpe lgkpsdggfl skmsklpkkv lphgskgeis agevsevsge
     4561 vpvelpearl kvgvkgperp dsdvdisvsd kkvtldvtkg fegkvevstp dlgkpsdkgf
     4621 iskvtkfpkk mfphgskggv svgevsevtg dvhvelpdah lkvgvkgper pdsdvdvsma
     4681 vkkitvdvpe gfegklevst pelarspdgs lvskaaklpk klvpsgensl ssveepslsa
     4741 hvdvpgsdtt fkvdaklpam pvtdvdisls akessadipv gvagkveigt pdagkpsdkg
     4801 fmskmakipk kmfhhgpkss gstdehstva etvddkalgf evqlqkdvki ptkpeadvsv
     4861 sfhekevtvq lpegregtve lstpglvgeh vpdkssqgad vllpgkelpt vegqeiiekp
     4921 ggvievievt ahelskplss gllskvskip kkifprgsks kssdedspsp gkrelketpg
     4981 givevvevtk hdvaihpest hvkqvgtevl lepatsetkp sgaeglppth itreikevpg
     5041 givevvrvtk hivgklpedk gigepsvrtt daslpweael akdpsavikr evketpeghv
     5101 evvqvirrea sdvpfgsdfp gdlesdgtvs rriettvvdg kptvirrlvt ttsdgirqvt
     5161 ehieqgaeda gsaasslfak mgefakglss ddpsavttre vkqlpggave vvsvtkrsss
     5221 speteitsqe qlqsstsgsm hfaesavgkp meakttiirr eisgpetivr tttsvvtsat
     5281 apgsskpels advldkmfga mekprvddrl rdvltaeggy vakrevtpee aellaqlfpe
     5341 qstgvavypp aglvpeaaii peyrcmttae gahhdpeske kvllklgral sqddldapsp
     5401 tqgeaamtva glgeavdtsd qveepavekl elarreskry sqefeqdepl pkamgfktkp
     5461 seetvvsstq vkgvhdngtv khtvttvtkt tiiqeveepa gaprpgeset wasdldtsqv
     5521 svsslpeder teptlsssat tatadaeals gplartqvee kewtetdpds gevrhiiskt
     5581 thttvtskkp segaprgdtt sttvshsskd ghhgstpsap secdeaeslt ffqkkeffek
     5641 lseqsppket ssarlgstps ldtaltrpav tlkprprses ltsqgeiiee sivfaerlrl
     5701 fqngaqaspl psasipqvdl vdpapkmeqk lqgmaeigds lqqvpsavle glqsteeksd
     5761 trllvhtsda dkeafdvrsv qvaedfgdkq fshmrvrmpa peetvsktsi cssdlpgdkp
     5821 splrdaykgs lsasrqessq agkpplpsal ggdsslclvh eattdltlsk agppnqtnkd
     5881 ittfdrqpvr eqsptlrssq sqmlasglqm givagsgilq egikaspqsa iletfsssat
     5941 emtglespei rglpseevha gsaeeakgid agrigavlaa apvailpvrd iisgkqleml
     6001 diaqvsdepn ifegcvelps qaesqtcpke ktimkteyad iqhklssafg eslapvesks
     6061 gvtqgvpkev lealdrssqe liaskldeds erdtnilsat qgsiphdvfp dhiestldns
     6121 aavpmrgays tpsstavclt perleiqatr evaeerqekp evlvaleayr kstlrrsged
     6181 sydrnleefi tvleeyeplp agqlsdwkra spafepsich nseitsklpp ledkkeletv
     6241 gsildpsenv sellgnhaqs qmlcedtgll tdelgrtagh yvistaesdr geclptlseq
     6301 apgkereecl nydsalgvrs tsglesslle esheersldl arsedeqpsl vedsmnvpte
     6361 ssvidyqvvg hsgvvkdaqt qpkdsdfdlh eisrtgdktd lplscgddgk ksaefsktvs
     6421 giaadmvikd tdsehlsqdm lhptastvlc egrsdvpyss dedqhkelsr ykevelvipf
     6481 lrgtsssled evedggsfss gdlqaekiae flttdpdtln kvvhfpqifq dydtsahqep
     6541 fvptetemnv ekhvgfvlde maacstpcvp lksdrqteie easrivesvv hiasslvesl
     6601 hevnhetaik edicdsalay aagsplerse vsetlsqagp pstiekdqre spfgvkemyq
     6661 vlkesrgsed riqysvtsfg dplfadegde dqerrrrgsp taeslddqaq iekcshevmm
     6721 qlldgfsqsd velaevrted eapdieddvp speagsheek svpssqdnsg dlqpqlstev
     6781 eigsqasgap aplseldyel cpdadqlrts gdeavatrvp pleperdeav aesshapsaa
     6841 adpaasagrn aggrerspll easppeetay cpeqdispaq eypeyltpdm qvrvgvspte
     6901 aqpakekpvr ysessvesss evgspcppsq ggtamafenv afagedvida tsspieesss
     6961 pagsrspyev vrdahaeeaa qtalveaeks lrhavqeevr arpqslvtee sseqqehqiq
     7021 psifneafdh speesapekk qlsegllgvg lltvsdttar tpesrrgdsd edeeewadhv
     7081 mskirgrpip ptppasprav qsavevqgep weapvveqer vnvesardms aerdagvdvm
     7141 gdrsatqpql qefrrtadgk rpqlehipse isdedlvere qspthitatc rgdrkdsssd
     7201 rnsspdsdgp kasgshrrsa sgvgaialea vhsqdsggne khshesrdkg aqeddkitee
     7261 pvdtmfviee eapadsnrse sfcddadera artagglsps lesppqkkdl ddgsssgkpd
     7321 aaspvvlrsk skrtdkisdk rssrdldtgh sgsetdhshy ysfeltsdsg ktpgasrpts
     7381 sefdvnilsg qasseyetca tsqdgevsgs yataaasshd tsfatarssl sgssrssays
     7441 vdsessghlg smevseaset ivasaheeld sdiesersks vlrepydgei pesvirggve
     7501 hpflpswqpa lkdssptlhg sagarpptae gvfspfqstv psepgrscvg wpssslstae
     7561 svrspskpev lngvsapleg qrrlrhnddf massheeeql svsessatqs ehtwqtsvet
     7621 mvdaveaqrf scdserhhrq stmqnsvslp fdeavvaeds hreadddrat ppfqevngpv
     7681 eveyvpeydd ltartlrpvr qqnapdgqte aqgdvallea sagdastsre qheeenstel
     7741 reegfyaetf qgdlsseeve saaneallld ksacsfyeyc hakdeyevcn trpavesasl
     7801 keaferpatp epvdeiddqe degnlaqgsp nlhgkdyeaf ghasaeesvp csdkthiaqe
     7861 lsendqykvf sdsgnqsslh detrsksvss sleramtddv rlytskcedy ilqmhqtyky
     7921 gvglpktsdg gylpeegmdv envkllesrl qegnggqqdf pydlsyqqey ggdgrvehgd
     7981 lythqeveee gyddersrtr grrrlspisd ergstpdydt lagrktfgkg sekddastss
     8041 lmeferleae masgtksssv gssdslsgrq hqgrsgagse qdsvsvnslt eferlekeiv
     8101 eaegvdarlr gevaakldei deghesqasd pghetavyde sgatlllcas advneepdgt
     8161 tddaqlgdie aaqgqevaws rterhflttr tesghsvesv drqssastat qfdadslrer
     8221 eiddsesnfg pdtpgwmqke cvdldslhds lhdsehhdsl qevpskdkds lqlrepsada
     8281 dslqedhnel qdslyetrdi dwdsfradet sglddtsrsw hqfpsqirtt elsptlsrpa
     8341 ssplvvpvvv eridtltwvd qdpakacvsd geslgsrqqe hprndalvss tdslepsssa
     8401 nthatyqcdt dsmmsslnsa eestmvtstd tlehetlcla sqgkslsleg glfsggdvte
     8461 eqntrtpleg gvsvddmeer frecllrsaa sptneeqvee rqtmdekgnl ivtrtvkrhv
     8521 tseprlhtqt fmgpdaeqrs refvasfaam epsaerdeye gfdaagntvr vtqhvvvrpt
     8581 iktvtftgpd arlqmreymr slaggqhssa entptsdsgv gacsslaspp plmpdppaaa
     8641 pssacpglqr lsggdqwmea esalddcspp vtatrvittr ttrtmgpdgr eqlvttttee
     8701 sggeepearl rrsmqgvlds fmaepptpph ldeee
//
LOCUS       XP_077503794            8729 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503794
VERSION     XP_077503794.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647668.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8729
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..8729
                     /product="uncharacterized protein LOC144114148 isoform X4"
                     /calculated_mol_wt=937758
     Region          59..90
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          73..390
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(92,94,98..99,102..104,106..107,111,114,123,125,127,
                     131..132,135..137,139..140,144,147,156,158,160,164..165,
                     168..170,172..173,177,180)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          92..123
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          125..156
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          158..183
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          191..216
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          224..251
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(253,255,259..260,263..265,267..268,272,275,284,286,
                     288,292..293,296..298,300..301,305,308,317,319,321,
                     325..326,329..331,333..334,338,341)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          253..284
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          286..317
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          319..349
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          333..621
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          352..416
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          418..449
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(451,453,457..458,461..463,465..466,470,473,482,484,
                     486,490..491,494..496,498..499,503,506,515,517,519,
                     523..524,527..529,531..532,536,539)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          451..482
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          484..515
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          499..777
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          517..546
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          550..575
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          583..614
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          616..649
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          650..680
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          682..712
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(715,717,721..722,725..727,729..730,734,737,746,748,
                     750,754..755,758..760,762..763,767,770,779,781,783,
                     787..788,791..793,795..796,800,803)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          715..746
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          748..779
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          753..834
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          781..811
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          992..1094
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1317..1449
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1481..1564
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <4492..4752
                     /region_name="YbbR"
                     /note="Cyclic di-AMP synthase regulator CdaR, YbbR domain
                     [Signal transduction mechanisms]; COG4856"
                     /db_xref="CDD:443884"
     Region          <7157..7470
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..8729
                     /gene="LOC144114148"
                     /coded_by="XM_077647668.1:884..27073"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 mgpgeptirq sreswigcli rllasqndqc dpsvylflrw ltllllravl sqmmppatdg
       61 nasflraara gnlekvldyl kgsidintsn anglnalhla akeghvnvvs ellkrganvn
      121 attkkgnsal hiaslagqee vvkllvqkqa nvnvqsqngf tplymaaqen hdgvvrflla
      181 nganqslate dgftplaval qqghdkvvav llendargkv rlpalhiask kddckaaall
      241 lhsehnpdvt sksgftplhi aahygnsnia sllldkgadv nfpakhqitp lhvaakwgks
      301 nmvklllekg akidastrdg ltplhcaars ghdqvveqll eknapitakt knglaplhma
      361 sqgdhvdsar illyhkapvd dvtvdyltal hvaahcghvg vakllldrra dpnaralngf
      421 tplhiackkn rikvvelllk hgasieatte sgltplhvas fmgcmniviy liqhganpdi
      481 ptvrgetplh laaranqtdi irillrngah vdakarelqt alhiasrlgn admvglllqh
      541 gaavdaptkd aytplhvaar egqdevaall ldhgaalaap tkkgftplhl aakygnlkva
      601 qlllqkdapv daqgkngvtp lhvaahydhv nvallllekg asphsaarng ytplhvaark
      661 dqmdiassll eygakpgaes ragftplhla aqeghadlaa llvehgadcd akakngltpm
      721 hlcaqedrve vatilakhga sldpttkagy tplhvachfg qtnmirfllr qganvnatts
      781 hgytplhqaa qqghtliinl llehraapna vtnqgqtala iaqrlgyisv vetlkvvtet
      841 ivttttttvt eekyrvvape tmhetfmsds edeaaednml gdqsfrylta demkslgdds
      901 lpidvtrder itesihitre pghpapltqe eerlsptqah tteaifvgny apdnvdlsrt
      961 pihagsllsw dgdscssayv lsptkrmwre rflvsflvda rggamrgcrh sgvrviippr
     1021 kaqmpmritc rylrkeklph pppllegeac asrilevgpa gakflgpvil evphfaslrg
     1081 kereisilrs dngetwkeht leaseeavqe vlnesfegee lsaledlqtn rivrilttdf
     1141 pqyfavvsrt rqevhavgpe ggllsstvvp qvqaifpega ltkkirvglq aqpipaelvt
     1201 kllghrvavs pivtveprrr kfhkpitlti pvpqaatkgm inqyagdapt lrllcsitgg
     1261 tnkaqwedvt gstpltfvnd cvsftttvsa rfwlmdcrqv seatkfatel yaeamhvpfm
     1321 akfvvfakrq dpmeaqlrvf cmtddkedkt lesqehftev aksrdvevle kksqylefgg
     1381 nlvpvtksge qlqlqfqafr enrlpfavrv kdphqeplgr lafmrqpraa rateppqapl
     1441 cnlnialpey easqnlselv tlekkygfve etglakpeli hradlrlsdi arelgsdwpa
     1501 laaqldvpeq dvasirtecp sdlaqqallm lrlwmkragg ratgnnlekg lrminrediv
     1561 nkcmfnvelv tddvekavak vhldqsgfdt freelgtpkd tslkrdasld vsydeqdlmk
     1621 eaesaeetss etgsvhekht gseakgtska ipgvrkgspi saaigrivrg sskekaaprm
     1681 aveetfeeep vlkidsaevt qqaapeagpt apprdskkks kkekspsplg pdyslprmrd
     1741 yhtperdfts ttdtlivssi kedvdaskel atdgkvkkek kkekkeksps ppgpdytlpr
     1801 lrdtytppsd vtaagyaast lpgqgitleq givkeplerk skerslspgv vktgengslg
     1861 adpsverqvk qlsqgpedti yekvervvke tpgkivemvh ikrqrssksp egrgspkdkn
     1921 kglpssevva pdisgesrsv psetigaekk qkkhketspt kqgkkeakev seavdkdael
     1981 sasdgklpds gflskmaklp kkmfprsgka sasdeepssl sesspvakkk agklpksatl
     2041 pkakpsktpl yeiksvdvev dtskltskgv kgvpakdgeg letsrdkepd srffskmari
     2101 pkkmfprssk tsssedepss asenlpsskq rakkekeiss kkkekvvkvk pdskklstsf
     2161 evevdrdtlp gagvdinakk pglqeslese srkktdsmqi sfpqsvkavg skgepmeysl
     2221 diegmkslqk epqlavvgef pgkpspdiel sltsketape fsdkarghie igtpehgkss
     2281 kggfmtkmak lprkmfphgf kgdvsveeps veaesvhvsv peaqvklgtk dlkkkgpdmp
     2341 ameltaslpv gaegsvemsa pdlpvsadgg liskvtqfpk mfshdsqdgv tierppvvge
     2401 gidvesslpd vqlkvkgkaf ekpetkfdvt lperkvtpvl pdgaeghiev nldemgksyd
     2461 dgfmskmakl pkkmfshgle deiavqepsi tqhdihvdip klkdeleira tlpekpqvdf
     2521 dvaipgkqvs ldlpegvesk ievsapdvgk ptdggfmskv vqlpkkmfph gskgtvplee
     2581 apeavgdiha kvpeahlkvg lkglekpdfe gdiavpgkkg avslpegteg kievstpetg
     2641 kpsdkgfmsk maklpkkmfp hgskgsvsve evpevdrsvk vkvpqgkipe ahlkvdikdp
     2701 ekhkdvdial seekvdidlp egidknievt mpevskpseg gfiskmakfp kkmfphgakg
     2761 svsveeapgi dvdvkmpetr ismdvkglek pkgevdislp kekvdidvpk gfdgklemsa
     2821 pevgkpsegg fmskmakfpk kmfphgakgs vsveeapgid vdvkvpeahi smdvkglekp
     2881 rgevdislpk ekvdidvpkg fdgklemsap evgkpseggf mskmakfpkk mfphgakgsl
     2941 sveetpgidv dvkmpeadls mdvkglekpk aeldiampkg kididmpegv dgkleasapd
     3001 igkpsdggfi skmtkfpkkm fphgakgtvs veapelgdvq vnmpeahlgv dikgpekpkg
     3061 eldvtlpkgk vdidlpegie gkveisapei gkpdggfmsk maklpkkmfp hgakggvsve
     3121 gspelgdvev kmpeahlgvd ikgpekpkge ldvtlpkgkv didlpegieg kveisapeig
     3181 kpdggfmskm aklpkkmfph gakggvsveg spelgdvevk mpeahlgvdi kgpekpkgel
     3241 dvtlpkgkvd idlpegiegk veisapeigk pdggfmskma klpkkmfphg akggvsvegs
     3301 pelgdvevkm peahlgvdik gpekpkgeld vtlpkgkvdi dlpegiegkv eisapeigkp
     3361 dggfmskmak lpkkmfshga kggvsvegsp elgdvqvkmp eahlgvdikg pekpkgeldv
     3421 tlpkgkvdid lpegiegkve isspeigkpd ggfmskmakl pkkmfphgak ggvsvegape
     3481 lgdvevkmpe ahlgvdikgp ekpkgeldvt lpkgkvdidl pegiegkvei sapeigkpdg
     3541 gfmskmaklp kkmfphgakg gvsvegapel gdvevkmpea hlgvdikgpe kpkgeldvtl
     3601 pkgkvdidlp egfegkveis apeigkpdgg fmskmaklpk kmfphgtkgg vsvegapelg
     3661 dvevkmpeah lgvdikgpek pkgeldvtlp kgkvdidlpe giegkveiss peigkpdggf
     3721 mskmaklpkk mfphgakggv svegapelgd vevkmpeahl gvdikgpekp kgeldvtlpk
     3781 gkvdidlpeg iegkveisap eigkpdggfm skmaklpkkm fphgakggvs vegspelgdv
     3841 evkmpeahlg vdikgpekpk geldvtlpkg kvdidlpegi egkleisape igkpdggfms
     3901 kmakfpkkmf phgakggvsv egapelgdvq vkmpeahlgv dikgpekpkg eldvtlpkgk
     3961 vdidlpegie gkveisapei gkpdggfmsk makfpkkmfp hgakggvsve gapelgdvqv
     4021 kmpeahlgvd iegpekpkge ldvtlpkgkv didlpegieg kveisapeig kpdggfmskm
     4081 akfpkkmfph gtkggvsveg apelgdvevk mpeahlgvdi kgpekpkgel dvtlpkgkvd
     4141 idlpegiegk veisapeigk pdggfmskma klpqkmfphg tkggvsvega pelgdiqlkm
     4201 peahlgvdik gpekpkgeld amlpkgkvdi dlpegiegkv eisapeigkp dggfmskmak
     4261 lpkkmfshga kgsisvegsp efgdvevkmp eahlggdikg pekpkgeldv alpkgkvdid
     4321 ipegiegkve isapeigkps dggfvskmak lpkkmfphgt kgsvsiegap evgdvqvkvp
     4381 eaqlkmdikg tgkpefegei twtgkkvave lpegiegtve mstpemgrps dggfmskvak
     4441 lpkkvfshgs kggvpfdeet gvagdievkm seaalkvdvk ypekpesdfs alekkididv
     4501 pegfkgkvei stpelgkpsd ggflskmskl pkkvlphgsk geisagevse vsgevpvelp
     4561 earlkvgvkg perpdsdvdi svsdkkvtld vtkgfegkve vstpdlgkps dkgfiskvtk
     4621 fpkkmfphgs kggvsvgevs evtgdvhvel pdahlkvgvk gperpdsdvd vsmavkkitv
     4681 dvpegfegkl evstpelars pdgslvskaa klpkklvpsg enslssveep slsahvdvpg
     4741 sdttfkvdak lpampvtdvd islsakessa dipvgvagkv eigtpdagkp sdkgfmskma
     4801 kipkkmfhhg pkssgstdeh stvaetvddk algfevqlqk dvkiptkpea dvsvsfheke
     4861 vtvqlpegre gtvelstpgl vgehvpdkss qgadvllpgk elptvegqei iekpggviev
     4921 ievtahelsk plssgllskv skipkkifpr gskskssded spspgkrelk etpggivevv
     4981 evtkhdvaih pesthvkqvg tevllepats etkpsgaegl ppthitreik evpggivevv
     5041 rvtkhivgkl pedkgigeps vrttdaslpw eaelakdpsa vikrevketp eghvevvqvi
     5101 rreasdvpfg sdfpgdlesd gtvsrriett vvdgkptvir rlvtttsdgi rqvtehieqg
     5161 aedagsaass lfakmgefak glssddpsav ttrevkqlpg gavevvsvtk rsssspetei
     5221 tsqeqlqsst sgsmhfaesa vgkpmeaktt iirreisgpe tivrtttsvv tsatapgssk
     5281 pelsadvldk mfgamekprv ddrlrdvlta eggyvakrev tpeeaellaq lfpeqstgva
     5341 vyppaglvpe aaiipeyrcm ttaegahhdp eskekvllkl gralsqddld apsptqgeaa
     5401 mtvaglgeav dtsdqveepa veklelarre skrysqefeq deplpkamgf ktkpseetvv
     5461 sstqvkgvhd ngtvkhtvtt vtkttiiqev eepagaprpg esetwasdld tsqvsvsslp
     5521 ederteptls ssattatada ealsgplart qveekewtet dpdsgevrhi isktthttvt
     5581 skkpsegapr gdttsttvsh sskdghhgst psapsecdea esltffqkke ffeklseqsp
     5641 pketssarlg stpsldtalt rpavtlkprp rsesltsqge iieesivfae rlrlfqngaq
     5701 asplpsasip qvdlvdpapk meqklqgmae igdslqqvps avleglqste eksdtrllvh
     5761 tsdadkeafd vrsvqvaedf gdkqfshmrv rmpapeetvs ktsicssdlp gdkpsplrda
     5821 ykgslsasrq essqagkppl psalggdssl clvheattdl tlskagppnq tnkdittfdr
     5881 qpvreqsptl rssqsqmlas glqmgivags gilqegikas pqsailetfs ssatemtgle
     5941 speirglpse evhagsaeea kgidagriga vlaaapvail pvrdiisgkq lemldiaqvs
     6001 depnifegcv elpsqaesqt cpkektimkt eyadiqhkls safgeslapv esksgvtqgv
     6061 pkevlealdr ssqeliaskl dedserdtni lsatqgsiph dvfpdhiest ldnsaavpmr
     6121 gaystpssta vcltperlei qatrevaeer qekpevlval eayrkstlrr sgedsydrnl
     6181 eefitvleey eplpagqlsd wkraspafep sichnseits klppledkke letvgsildp
     6241 senvsellgn haqsqmlced tglltdelgr taghyvista esdrgeclpt lseqapgker
     6301 eeclnydsal gvrstsgles slleesheer sldlarsede qpslvedsmn vptessvidy
     6361 qvvghsgvvk daqtqpkdsd fdlheisrtg dktdlplscg ddgkksaefs ktvsgiaadm
     6421 vikdtdsehl sqdmlhptas tvlcegrsdv pyssdedqhk elsrykevel vipflrgtss
     6481 sledevedgg sfssgdlqae kiaeflttdp dtlnkvvhfp qifqdydtsa hqepfvptet
     6541 emnvekhvgf vldemaacst pcvplksdrq teieeasriv esvvhiassl veslhevnhe
     6601 taikedicds alayaagspl ersevsetls qagppstiek dqrespfgvk emyqvlkesr
     6661 gsedriqysv tsfgdplfad egdedqerrr rgsptaesld dqaqiekcsh evmmqlldgf
     6721 sqsdvelaev rtedeapdie ddvpspeags heeksvpssq dnsgdlqpql steveigsqa
     6781 sgapaplsel dyelcpdadq lrtsgdeava trvppleper deavaessha psaaadpaas
     6841 agrnaggrer splleasppe etaycpeqdi spaqeypeyl tpdmqvrvgv spteaqpake
     6901 kpvrysessv esssevgspc ppsqggtama fenvafaged vidatsspie essspagsrs
     6961 pyevvrdaha eeaaqtalve aekslrhavq eevrarpqsl vteesseqqe hqiqpsifne
     7021 afdhspeesa pekkqlsegl lgvglltvsd ttartpesrr gdsdedeeew adhvmskirg
     7081 rpipptppas pravqsavev qgepweapvv eqervnvesa rdmsaerdag vdvmgdrsat
     7141 qpqlqefrrt adgkrpqleh ipseisdedl vereqspthi tatcrgdrkd sssdrnsspd
     7201 sdgpkasgsh rrsasgvgai aleavhsqds ggnekhshes rdkgaqeddk iteepvdtmf
     7261 vieeeapads nrsesfcdda deraartagg lspslesppq kkdlddgsss gkpdaaspvv
     7321 lrskskrtdk isdkrssrdl dtghsgsetd hshyysfelt sdsgktpgas rptssefdvn
     7381 ilsgqassey etcatsqdge vsgsyataaa sshdtsfata rsslsgssrs saysvdsess
     7441 ghlgsmevse asetivasah eeldsdiese rsksvlrepy dgeipesvir ggvehpflps
     7501 wqpalkdssp tlhgsagarp ptaegvfspf qstvpsepgr scvgwpsssl staesvrsps
     7561 kpevlngvsa plegqrrlrh nddfmasshe eeqlsvsess atqsehtwqt svetmvdave
     7621 aqrfscdser hhrqstmqns vslpfdeavv aedshreadd dratppfqev ngpveveyvp
     7681 eyddltartl rpvrqqnapd gqteaqgdva lleasagdas tsreqheeen stelreegfy
     7741 aetfqgdlss eevesaanea llldksacsf yeychakdey evcntrpave saslkeafer
     7801 patpepvdei ddqedegnla qgspnlhgkd yeafghasae esvpcsdkth iaqelsendq
     7861 ykvfsdsgnq sslhdetrsk svsssleram tddvrlytsk cedyilqmhq tykygvglpk
     7921 tsdggylpee gmdvenvkll esrlqegngg qqdfpydlsy qqeyggdgrv ehgdlythqe
     7981 veeegydder srtrgrrrls pisdergstp dydtlagrkt fgkgsekdda stsslmefer
     8041 leaemasgtk sssvgssdsl sgrqhqgrsg agseqdsvsv nslteferle keiveaegvd
     8101 arlrgevaak ldeideghes qasdpgheta vydesgatll lcasadvnee pdgttddaql
     8161 gdieaaqgqe vawsrterhf lttrtesghs vesvdrqssa statqfdads lrereiddse
     8221 snfgpdtpgw mqkecvdlds lhdslhdseh hdslqevpsk dkdslqlrep sadadslqed
     8281 hnelqdslye trdidwdsfr adetsglddt srswhqfpsq irttelsptl srpassplvv
     8341 pvvveridtl twvdqdpaka cvsdgeslgs rqqehprnda lvsstdslep sssanthaty
     8401 qcdtdsmmss lnsaeestmv tstdtlehet lclasqgksl slegglfsgg dvteeqntrt
     8461 pleggvsvdd meerfrecll rsaasptnee qveerqtmde kgnlivtrtv krhvtseprl
     8521 htqtfmgpda eqrsrefvas faamepsaer deyegfdaag ntvrvtqhvv vrptiktvtf
     8581 tgpdarlqmr eymrslaggq hssaentpts dsgvgacssl asppplmpdp paaapssacp
     8641 glqrlsggdq wmeaesaldd csppvtatrv ittrttrtmg pdgreqlvtt tteesggeep
     8701 earlrrsmqg vldsfmaepp tpphldeee
//
LOCUS       XP_077503796            8728 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077503796
VERSION     XP_077503796.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647670.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8728
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..8728
                     /product="uncharacterized protein LOC144114148 isoform X5"
                     /calculated_mol_wt=937629
     Region          59..90
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          73..390
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(92,94,98..99,102..104,106..107,111,114,123,125,127,
                     131..132,135..137,139..140,144,147,156,158,160,164..165,
                     168..170,172..173,177,180)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          92..123
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          125..156
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          158..183
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          191..216
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          224..251
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(253,255,259..260,263..265,267..268,272,275,284,286,
                     288,292..293,296..298,300..301,305,308,317,319,321,
                     325..326,329..331,333..334,338,341)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          253..284
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          286..317
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          319..349
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          333..621
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          352..416
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          418..449
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(451,453,457..458,461..463,465..466,470,473,482,484,
                     486,490..491,494..496,498..499,503,506,515,517,519,
                     523..524,527..529,531..532,536,539)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          451..482
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          484..515
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          499..777
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          517..546
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          550..575
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          583..614
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          616..649
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          650..680
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          682..712
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(715,717,721..722,725..727,729..730,734,737,746,748,
                     750,754..755,758..760,762..763,767,770,779,781,783,
                     787..788,791..793,795..796,800,803)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          715..746
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          748..779
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          753..834
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          781..811
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          992..1094
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1317..1449
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1481..1564
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <1877..2128
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          <4491..4751
                     /region_name="YbbR"
                     /note="Cyclic di-AMP synthase regulator CdaR, YbbR domain
                     [Signal transduction mechanisms]; COG4856"
                     /db_xref="CDD:443884"
     Region          <7156..7469
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..8728
                     /gene="LOC144114148"
                     /coded_by="XM_077647670.1:883..27069"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 mgpgeptirq sreswigcli rllasqndqc dpsvylflrw ltllllravl sqmmppatdg
       61 nasflraara gnlekvldyl kgsidintsn anglnalhla akeghvnvvs ellkrganvn
      121 attkkgnsal hiaslagqee vvkllvqkqa nvnvqsqngf tplymaaqen hdgvvrflla
      181 nganqslate dgftplaval qqghdkvvav llendargkv rlpalhiask kddckaaall
      241 lhsehnpdvt sksgftplhi aahygnsnia sllldkgadv nfpakhqitp lhvaakwgks
      301 nmvklllekg akidastrdg ltplhcaars ghdqvveqll eknapitakt knglaplhma
      361 sqgdhvdsar illyhkapvd dvtvdyltal hvaahcghvg vakllldrra dpnaralngf
      421 tplhiackkn rikvvelllk hgasieatte sgltplhvas fmgcmniviy liqhganpdi
      481 ptvrgetplh laaranqtdi irillrngah vdakarelqt alhiasrlgn admvglllqh
      541 gaavdaptkd aytplhvaar egqdevaall ldhgaalaap tkkgftplhl aakygnlkva
      601 qlllqkdapv daqgkngvtp lhvaahydhv nvallllekg asphsaarng ytplhvaark
      661 dqmdiassll eygakpgaes ragftplhla aqeghadlaa llvehgadcd akakngltpm
      721 hlcaqedrve vatilakhga sldpttkagy tplhvachfg qtnmirfllr qganvnatts
      781 hgytplhqaa qqghtliinl llehraapna vtnqgqtala iaqrlgyisv vetlkvvtet
      841 ivttttttvt eekyrvvape tmhetfmsds edeaaednml gdqsfrylta demkslgdds
      901 lpidvtrder itesihitre pghpapltqe eerlsptqah tteaifvgny apdnvdlsrt
      961 pihagsllsw dgdscssayv lsptkrmwre rflvsflvda rggamrgcrh sgvrviippr
     1021 kaqmpmritc rylrkeklph pppllegeac asrilevgpa gakflgpvil evphfaslrg
     1081 kereisilrs dngetwkeht leaseeavqe vlnesfegee lsaledlqtn rivrilttdf
     1141 pqyfavvsrt rqevhavgpe ggllsstvvp qvqaifpega ltkkirvglq aqpipaelvt
     1201 kllghrvavs pivtveprrr kfhkpitlti pvpqaatkgm inqyagdapt lrllcsitgg
     1261 tnkaqwedvt gstpltfvnd cvsftttvsa rfwlmdcrqv seatkfatel yaeamhvpfm
     1321 akfvvfakrq dpmeaqlrvf cmtddkedkt lesqehftev aksrdvevle kksqylefgg
     1381 nlvpvtksge qlqlqfqafr enrlpfavrv kdphqeplgr lafmrqpraa rateppqapl
     1441 cnlnialpey easqnlselv tlekkygfve etglakpeli hradlrlsdi arelgsdwpa
     1501 laaqldvpeq dvasirtecp sdlaqqallm lrlwmkragg ratgnnlekg lrminrediv
     1561 nkcmfnvelv tddvekavak vhldqsgfdt freelgtpkd tslkrdasld vsydeqdlmk
     1621 aesaeetsse tgsvhekhtg seakgtskai pgvrkgspis aaigrivrgs skekaaprma
     1681 veetfeeepv lkidsaevtq qaapeagpta pprdskkksk kekspsplgp dyslprmrdy
     1741 htperdftst tdtlivssik edvdaskela tdgkvkkekk kekkekspsp pgpdytlprl
     1801 rdtytppsdv taagyaastl pgqgitleqg ivkeplerks kerslspgvv ktgengslga
     1861 dpsverqvkq lsqgpedtiy ekvervvket pgkivemvhi krqrsskspe grgspkdknk
     1921 glpssevvap disgesrsvp setigaekkq kkhketsptk qgkkeakevs eavdkdaels
     1981 asdgklpdsg flskmaklpk kmfprsgkas asdeepssls esspvakkka gklpksatlp
     2041 kakpsktply eiksvdvevd tskltskgvk gvpakdgegl etsrdkepds rffskmarip
     2101 kkmfprsskt sssedepssa senlpsskqr akkekeissk kkekvvkvkp dskklstsfe
     2161 vevdrdtlpg agvdinakkp glqesleses rkktdsmqis fpqsvkavgs kgepmeysld
     2221 iegmkslqke pqlavvgefp gkpspdiels ltsketapef sdkarghiei gtpehgkssk
     2281 ggfmtkmakl prkmfphgfk gdvsveepsv eaesvhvsvp eaqvklgtkd lkkkgpdmpa
     2341 meltaslpvg aegsvemsap dlpvsadggl iskvtqfpkm fshdsqdgvt ierppvvgeg
     2401 idvesslpdv qlkvkgkafe kpetkfdvtl perkvtpvlp dgaeghievn ldemgksydd
     2461 gfmskmaklp kkmfshgled eiavqepsit qhdihvdipk lkdeleirat lpekpqvdfd
     2521 vaipgkqvsl dlpegveski evsapdvgkp tdggfmskvv qlpkkmfphg skgtvpleea
     2581 peavgdihak vpeahlkvgl kglekpdfeg diavpgkkga vslpegtegk ievstpetgk
     2641 psdkgfmskm aklpkkmfph gskgsvsvee vpevdrsvkv kvpqgkipea hlkvdikdpe
     2701 khkdvdials eekvdidlpe gidknievtm pevskpsegg fiskmakfpk kmfphgakgs
     2761 vsveeapgid vdvkmpetri smdvkglekp kgevdislpk ekvdidvpkg fdgklemsap
     2821 evgkpseggf mskmakfpkk mfphgakgsv sveeapgidv dvkvpeahis mdvkglekpr
     2881 gevdislpke kvdidvpkgf dgklemsape vgkpseggfm skmakfpkkm fphgakgsls
     2941 veetpgidvd vkmpeadlsm dvkglekpka eldiampkgk ididmpegvd gkleasapdi
     3001 gkpsdggfis kmtkfpkkmf phgakgtvsv eapelgdvqv nmpeahlgvd ikgpekpkge
     3061 ldvtlpkgkv didlpegieg kveisapeig kpdggfmskm aklpkkmfph gakggvsveg
     3121 spelgdvevk mpeahlgvdi kgpekpkgel dvtlpkgkvd idlpegiegk veisapeigk
     3181 pdggfmskma klpkkmfphg akggvsvegs pelgdvevkm peahlgvdik gpekpkgeld
     3241 vtlpkgkvdi dlpegiegkv eisapeigkp dggfmskmak lpkkmfphga kggvsvegsp
     3301 elgdvevkmp eahlgvdikg pekpkgeldv tlpkgkvdid lpegiegkve isapeigkpd
     3361 ggfmskmakl pkkmfshgak ggvsvegspe lgdvqvkmpe ahlgvdikgp ekpkgeldvt
     3421 lpkgkvdidl pegiegkvei sspeigkpdg gfmskmaklp kkmfphgakg gvsvegapel
     3481 gdvevkmpea hlgvdikgpe kpkgeldvtl pkgkvdidlp egiegkveis apeigkpdgg
     3541 fmskmaklpk kmfphgakgg vsvegapelg dvevkmpeah lgvdikgpek pkgeldvtlp
     3601 kgkvdidlpe gfegkveisa peigkpdggf mskmaklpkk mfphgtkggv svegapelgd
     3661 vevkmpeahl gvdikgpekp kgeldvtlpk gkvdidlpeg iegkveissp eigkpdggfm
     3721 skmaklpkkm fphgakggvs vegapelgdv evkmpeahlg vdikgpekpk geldvtlpkg
     3781 kvdidlpegi egkveisape igkpdggfms kmaklpkkmf phgakggvsv egspelgdve
     3841 vkmpeahlgv dikgpekpkg eldvtlpkgk vdidlpegie gkleisapei gkpdggfmsk
     3901 makfpkkmfp hgakggvsve gapelgdvqv kmpeahlgvd ikgpekpkge ldvtlpkgkv
     3961 didlpegieg kveisapeig kpdggfmskm akfpkkmfph gakggvsveg apelgdvqvk
     4021 mpeahlgvdi egpekpkgel dvtlpkgkvd idlpegiegk veisapeigk pdggfmskma
     4081 kfpkkmfphg tkggvsvega pelgdvevkm peahlgvdik gpekpkgeld vtlpkgkvdi
     4141 dlpegiegkv eisapeigkp dggfmskmak lpqkmfphgt kggvsvegap elgdiqlkmp
     4201 eahlgvdikg pekpkgelda mlpkgkvdid lpegiegkve isapeigkpd ggfmskmakl
     4261 pkkmfshgak gsisvegspe fgdvevkmpe ahlggdikgp ekpkgeldva lpkgkvdidi
     4321 pegiegkvei sapeigkpsd ggfvskmakl pkkmfphgtk gsvsiegape vgdvqvkvpe
     4381 aqlkmdikgt gkpefegeit wtgkkvavel pegiegtvem stpemgrpsd ggfmskvakl
     4441 pkkvfshgsk ggvpfdeetg vagdievkms eaalkvdvky pekpesdfsa lekkididvp
     4501 egfkgkveis tpelgkpsdg gflskmsklp kkvlphgskg eisagevsev sgevpvelpe
     4561 arlkvgvkgp erpdsdvdis vsdkkvtldv tkgfegkvev stpdlgkpsd kgfiskvtkf
     4621 pkkmfphgsk ggvsvgevse vtgdvhvelp dahlkvgvkg perpdsdvdv smavkkitvd
     4681 vpegfegkle vstpelarsp dgslvskaak lpkklvpsge nslssveeps lsahvdvpgs
     4741 dttfkvdakl pampvtdvdi slsakessad ipvgvagkve igtpdagkps dkgfmskmak
     4801 ipkkmfhhgp kssgstdehs tvaetvddka lgfevqlqkd vkiptkpead vsvsfhekev
     4861 tvqlpegreg tvelstpglv gehvpdkssq gadvllpgke lptvegqeii ekpggvievi
     4921 evtahelskp lssgllskvs kipkkifprg skskssdeds pspgkrelke tpggivevve
     4981 vtkhdvaihp esthvkqvgt evllepatse tkpsgaeglp pthitreike vpggivevvr
     5041 vtkhivgklp edkgigepsv rttdaslpwe aelakdpsav ikrevketpe ghvevvqvir
     5101 reasdvpfgs dfpgdlesdg tvsrriettv vdgkptvirr lvtttsdgir qvtehieqga
     5161 edagsaassl fakmgefakg lssddpsavt trevkqlpgg avevvsvtkr sssspeteit
     5221 sqeqlqssts gsmhfaesav gkpmeaktti irreisgpet ivrtttsvvt satapgsskp
     5281 elsadvldkm fgamekprvd drlrdvltae ggyvakrevt peeaellaql fpeqstgvav
     5341 yppaglvpea aiipeyrcmt taegahhdpe skekvllklg ralsqddlda psptqgeaam
     5401 tvaglgeavd tsdqveepav eklelarres krysqefeqd eplpkamgfk tkpseetvvs
     5461 stqvkgvhdn gtvkhtvttv tkttiiqeve epagaprpge setwasdldt sqvsvsslpe
     5521 derteptlss sattatadae alsgplartq veekewtetd pdsgevrhii sktthttvts
     5581 kkpsegaprg dttsttvshs skdghhgstp sapsecdeae sltffqkkef feklseqspp
     5641 ketssarlgs tpsldtaltr pavtlkprpr sesltsqgei ieesivfaer lrlfqngaqa
     5701 splpsasipq vdlvdpapkm eqklqgmaei gdslqqvpsa vleglqstee ksdtrllvht
     5761 sdadkeafdv rsvqvaedfg dkqfshmrvr mpapeetvsk tsicssdlpg dkpsplrday
     5821 kgslsasrqe ssqagkpplp salggdsslc lvheattdlt lskagppnqt nkdittfdrq
     5881 pvreqsptlr ssqsqmlasg lqmgivagsg ilqegikasp qsailetfss satemtgles
     5941 peirglpsee vhagsaeeak gidagrigav laaapvailp vrdiisgkql emldiaqvsd
     6001 epnifegcve lpsqaesqtc pkektimkte yadiqhklss afgeslapve sksgvtqgvp
     6061 kevlealdrs sqeliaskld edserdtnil satqgsiphd vfpdhiestl dnsaavpmrg
     6121 aystpsstav cltperleiq atrevaeerq ekpevlvale ayrkstlrrs gedsydrnle
     6181 efitvleeye plpagqlsdw kraspafeps ichnseitsk lppledkkel etvgsildps
     6241 envsellgnh aqsqmlcedt glltdelgrt aghyvistae sdrgeclptl seqapgkere
     6301 eclnydsalg vrstsgless lleesheers ldlarsedeq pslvedsmnv ptessvidyq
     6361 vvghsgvvkd aqtqpkdsdf dlheisrtgd ktdlplscgd dgkksaefsk tvsgiaadmv
     6421 ikdtdsehls qdmlhptast vlcegrsdvp yssdedqhke lsrykevelv ipflrgtsss
     6481 ledevedggs fssgdlqaek iaeflttdpd tlnkvvhfpq ifqdydtsah qepfvptete
     6541 mnvekhvgfv ldemaacstp cvplksdrqt eieeasrive svvhiasslv eslhevnhet
     6601 aikedicdsa layaagsple rsevsetlsq agppstiekd qrespfgvke myqvlkesrg
     6661 sedriqysvt sfgdplfade gdedqerrrr gsptaesldd qaqiekcshe vmmqlldgfs
     6721 qsdvelaevr tedeapdied dvpspeagsh eeksvpssqd nsgdlqpqls teveigsqas
     6781 gapaplseld yelcpdadql rtsgdeavat rvppleperd eavaesshap saaadpaasa
     6841 grnaggrers plleasppee taycpeqdis paqeypeylt pdmqvrvgvs pteaqpakek
     6901 pvrysessve sssevgspcp psqggtamaf envafagedv idatsspiee ssspagsrsp
     6961 yevvrdahae eaaqtalvea ekslrhavqe evrarpqslv teesseqqeh qiqpsifnea
     7021 fdhspeesap ekkqlsegll gvglltvsdt tartpesrrg dsdedeeewa dhvmskirgr
     7081 pipptppasp ravqsavevq gepweapvve qervnvesar dmsaerdagv dvmgdrsatq
     7141 pqlqefrrta dgkrpqlehi pseisdedlv ereqspthit atcrgdrkds ssdrnsspds
     7201 dgpkasgshr rsasgvgaia leavhsqdsg gnekhshesr dkgaqeddki teepvdtmfv
     7261 ieeeapadsn rsesfcddad eraartaggl spslesppqk kdlddgsssg kpdaaspvvl
     7321 rskskrtdki sdkrssrdld tghsgsetdh shyysfelts dsgktpgasr ptssefdvni
     7381 lsgqasseye tcatsqdgev sgsyataaas shdtsfatar sslsgssrss aysvdsessg
     7441 hlgsmevsea setivasahe eldsdieser sksvlrepyd geipesvirg gvehpflpsw
     7501 qpalkdsspt lhgsagarpp taegvfspfq stvpsepgrs cvgwpsssls taesvrspsk
     7561 pevlngvsap legqrrlrhn ddfmasshee eqlsvsessa tqsehtwqts vetmvdavea
     7621 qrfscdserh hrqstmqnsv slpfdeavva edshreaddd ratppfqevn gpveveyvpe
     7681 yddltartlr pvrqqnapdg qteaqgdval leasagdast sreqheeens telreegfya
     7741 etfqgdlsse evesaaneal lldksacsfy eychakdeye vcntrpaves aslkeaferp
     7801 atpepvdeid dqedegnlaq gspnlhgkdy eafghasaee svpcsdkthi aqelsendqy
     7861 kvfsdsgnqs slhdetrsks vsssleramt ddvrlytskc edyilqmhqt ykygvglpkt
     7921 sdggylpeeg mdvenvklle srlqegnggq qdfpydlsyq qeyggdgrve hgdlythqev
     7981 eeegydders rtrgrrrlsp isdergstpd ydtlagrktf gkgsekddas tsslmeferl
     8041 eaemasgtks ssvgssdsls grqhqgrsga gseqdsvsvn slteferlek eiveaegvda
     8101 rlrgevaakl deideghesq asdpghetav ydesgatlll casadvneep dgttddaqlg
     8161 dieaaqgqev awsrterhfl ttrtesghsv esvdrqssas tatqfdadsl rereiddses
     8221 nfgpdtpgwm qkecvdldsl hdslhdsehh dslqevpskd kdslqlreps adadslqedh
     8281 nelqdslyet rdidwdsfra detsglddts rswhqfpsqi rttelsptls rpassplvvp
     8341 vvveridtlt wvdqdpakac vsdgeslgsr qqehprndal vsstdsleps ssanthatyq
     8401 cdtdsmmssl nsaeestmvt stdtlehetl clasqgksls legglfsggd vteeqntrtp
     8461 leggvsvddm eerfrecllr saasptneeq veerqtmdek gnlivtrtvk rhvtseprlh
     8521 tqtfmgpdae qrsrefvasf aamepsaerd eyegfdaagn tvrvtqhvvv rptiktvtft
     8581 gpdarlqmre ymrslaggqh ssaentptsd sgvgacssla sppplmpdpp aaapssacpg
     8641 lqrlsggdqw meaesalddc sppvtatrvi ttrttrtmgp dgreqlvttt teesggeepe
     8701 arlrrsmqgv ldsfmaeppt pphldeee
//
LOCUS       XP_077503797            8703 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077503797
VERSION     XP_077503797.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647671.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8703
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..8703
                     /product="uncharacterized protein LOC144114148 isoform X6"
                     /calculated_mol_wt=934745
     Region          59..90
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          73..390
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(92,94,98..99,102..104,106..107,111,114,123,125,127,
                     131..132,135..137,139..140,144,147,156,158,160,164..165,
                     168..170,172..173,177,180)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          92..123
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          125..156
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          158..183
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          191..216
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          224..251
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(253,255,259..260,263..265,267..268,272,275,284,286,
                     288,292..293,296..298,300..301,305,308,317,319,321,
                     325..326,329..331,333..334,338,341)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          253..284
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          286..317
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          319..349
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          333..621
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          352..416
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          418..449
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(451,453,457..458,461..463,465..466,470,473,482,484,
                     486,490..491,494..496,498..499,503,506,515,517,519,
                     523..524,527..529,531..532,536,539)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          451..482
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          484..515
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          499..777
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          517..546
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          550..575
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          583..614
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          616..649
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          650..680
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          682..712
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(715,717,721..722,725..727,729..730,734,737,746,748,
                     750,754..755,758..760,762..763,767,770,779,781,783,
                     787..788,791..793,795..796,800,803)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          715..746
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          748..779
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          753..834
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          781..811
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          965..1068
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1291..1423
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1455..1538
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <1852..2103
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          <4466..4726
                     /region_name="YbbR"
                     /note="Cyclic di-AMP synthase regulator CdaR, YbbR domain
                     [Signal transduction mechanisms]; COG4856"
                     /db_xref="CDD:443884"
     Region          <7131..7444
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..8703
                     /gene="LOC144114148"
                     /coded_by="XM_077647671.1:883..26994"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 mgpgeptirq sreswigcli rllasqndqc dpsvylflrw ltllllravl sqmmppatdg
       61 nasflraara gnlekvldyl kgsidintsn anglnalhla akeghvnvvs ellkrganvn
      121 attkkgnsal hiaslagqee vvkllvqkqa nvnvqsqngf tplymaaqen hdgvvrflla
      181 nganqslate dgftplaval qqghdkvvav llendargkv rlpalhiask kddckaaall
      241 lhsehnpdvt sksgftplhi aahygnsnia sllldkgadv nfpakhqitp lhvaakwgks
      301 nmvklllekg akidastrdg ltplhcaars ghdqvveqll eknapitakt knglaplhma
      361 sqgdhvdsar illyhkapvd dvtvdyltal hvaahcghvg vakllldrra dpnaralngf
      421 tplhiackkn rikvvelllk hgasieatte sgltplhvas fmgcmniviy liqhganpdi
      481 ptvrgetplh laaranqtdi irillrngah vdakarelqt alhiasrlgn admvglllqh
      541 gaavdaptkd aytplhvaar egqdevaall ldhgaalaap tkkgftplhl aakygnlkva
      601 qlllqkdapv daqgkngvtp lhvaahydhv nvallllekg asphsaarng ytplhvaark
      661 dqmdiassll eygakpgaes ragftplhla aqeghadlaa llvehgadcd akakngltpm
      721 hlcaqedrve vatilakhga sldpttkagy tplhvachfg qtnmirfllr qganvnatts
      781 hgytplhqaa qqghtliinl llehraapna vtnqgqtala iaqrlgyisv vetlkvvtet
      841 ivttttttvt eekyrvvape tmhetfmsds edeaaednml gdqsfrylta demkslgdds
      901 lpidvtrder itesihitre pghpapltqe eerlsptqah tteaifvgny apdnvdlsrt
      961 pihagflvsf lvdarggamr gcrhsgvrvi ipprkaqmpm ritcrylrke klphppplle
     1021 geacasrile vgpagakflg pvilevphfa slrgkereis ilrsdngetw kehtleasee
     1081 avqevlnesf egeelsaled lqtnrivril ttdfpqyfav vsrtrqevha vgpeggllss
     1141 tvvpqvqaif pegaltkkir vglqaqpipa elvtkllghr vavspivtve prrrkfhkpi
     1201 tltipvpqaa tkgminqyag daptlrllcs itggtnkaqw edvtgstplt fvndcvsftt
     1261 tvsarfwlmd crqvseatkf atelyaeamh vpfmakfvvf akrqdpmeaq lrvfcmtddk
     1321 edktlesqeh ftevaksrdv evlekksqyl efggnlvpvt ksgeqlqlqf qafrenrlpf
     1381 avrvkdphqe plgrlafmrq praaratepp qaplcnlnia lpeyeasqnl selvtlekky
     1441 gfveetglak pelihradlr lsdiarelgs dwpalaaqld vpeqdvasir tecpsdlaqq
     1501 allmlrlwmk raggratgnn lekglrminr edivnkcmfn velvtddvek avakvhldqs
     1561 gfdtfreelg tpkdtslkrd asldvsydeq dlmkeaesae etssetgsvh ekhtgseakg
     1621 tskaipgvrk gspisaaigr ivrgsskeka aprmaveetf eeepvlkids aevtqqaape
     1681 agptapprds kkkskkeksp splgpdyslp rmrdyhtper dftsttdtli vssikedvda
     1741 skelatdgkv kkekkkekke kspsppgpdy tlprlrdtyt ppsdvtaagy aastlpgqgi
     1801 tleqgivkep lerkskersl spgvvktgen gslgadpsve rqvkqlsqgp edtiyekver
     1861 vvketpgkiv emvhikrqrs skspegrgsp kdknkglpss evvapdisge srsvpsetig
     1921 aekkqkkhke tsptkqgkke akevseavdk daelsasdgk lpdsgflskm aklpkkmfpr
     1981 sgkasasdee psslsesspv akkkagklpk satlpkakps ktplyeiksv dvevdtsklt
     2041 skgvkgvpak dgegletsrd kepdsrffsk maripkkmfp rssktsssed epssasenlp
     2101 sskqrakkek eisskkkekv vkvkpdskkl stsfevevdr dtlpgagvdi nakkpglqes
     2161 lesesrkktd smqisfpqsv kavgskgepm eysldiegmk slqkepqlav vgefpgkpsp
     2221 dielsltske tapefsdkar ghieigtpeh gksskggfmt kmaklprkmf phgfkgdvsv
     2281 eepsveaesv hvsvpeaqvk lgtkdlkkkg pdmpamelta slpvgaegsv emsapdlpvs
     2341 adggliskvt qfpkmfshds qdgvtierpp vvgegidves slpdvqlkvk gkafekpetk
     2401 fdvtlperkv tpvlpdgaeg hievnldemg ksyddgfmsk maklpkkmfs hgledeiavq
     2461 epsitqhdih vdipklkdel eiratlpekp qvdfdvaipg kqvsldlpeg veskievsap
     2521 dvgkptdggf mskvvqlpkk mfphgskgtv pleeapeavg dihakvpeah lkvglkglek
     2581 pdfegdiavp gkkgavslpe gtegkievst petgkpsdkg fmskmaklpk kmfphgskgs
     2641 vsveevpevd rsvkvkvpqg kipeahlkvd ikdpekhkdv dialseekvd idlpegidkn
     2701 ievtmpevsk pseggfiskm akfpkkmfph gakgsvsvee apgidvdvkm petrismdvk
     2761 glekpkgevd islpkekvdi dvpkgfdgkl emsapevgkp seggfmskma kfpkkmfphg
     2821 akgsvsveea pgidvdvkvp eahismdvkg lekprgevdi slpkekvdid vpkgfdgkle
     2881 msapevgkps eggfmskmak fpkkmfphga kgslsveetp gidvdvkmpe adlsmdvkgl
     2941 ekpkaeldia mpkgkididm pegvdgklea sapdigkpsd ggfiskmtkf pkkmfphgak
     3001 gtvsveapel gdvqvnmpea hlgvdikgpe kpkgeldvtl pkgkvdidlp egiegkveis
     3061 apeigkpdgg fmskmaklpk kmfphgakgg vsvegspelg dvevkmpeah lgvdikgpek
     3121 pkgeldvtlp kgkvdidlpe giegkveisa peigkpdggf mskmaklpkk mfphgakggv
     3181 svegspelgd vevkmpeahl gvdikgpekp kgeldvtlpk gkvdidlpeg iegkveisap
     3241 eigkpdggfm skmaklpkkm fphgakggvs vegspelgdv evkmpeahlg vdikgpekpk
     3301 geldvtlpkg kvdidlpegi egkveisape igkpdggfms kmaklpkkmf shgakggvsv
     3361 egspelgdvq vkmpeahlgv dikgpekpkg eldvtlpkgk vdidlpegie gkveisspei
     3421 gkpdggfmsk maklpkkmfp hgakggvsve gapelgdvev kmpeahlgvd ikgpekpkge
     3481 ldvtlpkgkv didlpegieg kveisapeig kpdggfmskm aklpkkmfph gakggvsveg
     3541 apelgdvevk mpeahlgvdi kgpekpkgel dvtlpkgkvd idlpegfegk veisapeigk
     3601 pdggfmskma klpkkmfphg tkggvsvega pelgdvevkm peahlgvdik gpekpkgeld
     3661 vtlpkgkvdi dlpegiegkv eisspeigkp dggfmskmak lpkkmfphga kggvsvegap
     3721 elgdvevkmp eahlgvdikg pekpkgeldv tlpkgkvdid lpegiegkve isapeigkpd
     3781 ggfmskmakl pkkmfphgak ggvsvegspe lgdvevkmpe ahlgvdikgp ekpkgeldvt
     3841 lpkgkvdidl pegiegklei sapeigkpdg gfmskmakfp kkmfphgakg gvsvegapel
     3901 gdvqvkmpea hlgvdikgpe kpkgeldvtl pkgkvdidlp egiegkveis apeigkpdgg
     3961 fmskmakfpk kmfphgakgg vsvegapelg dvqvkmpeah lgvdiegpek pkgeldvtlp
     4021 kgkvdidlpe giegkveisa peigkpdggf mskmakfpkk mfphgtkggv svegapelgd
     4081 vevkmpeahl gvdikgpekp kgeldvtlpk gkvdidlpeg iegkveisap eigkpdggfm
     4141 skmaklpqkm fphgtkggvs vegapelgdi qlkmpeahlg vdikgpekpk geldamlpkg
     4201 kvdidlpegi egkveisape igkpdggfms kmaklpkkmf shgakgsisv egspefgdve
     4261 vkmpeahlgg dikgpekpkg eldvalpkgk vdidipegie gkveisapei gkpsdggfvs
     4321 kmaklpkkmf phgtkgsvsi egapevgdvq vkvpeaqlkm dikgtgkpef egeitwtgkk
     4381 vavelpegie gtvemstpem grpsdggfms kvaklpkkvf shgskggvpf deetgvagdi
     4441 evkmseaalk vdvkypekpe sdfsalekki didvpegfkg kveistpelg kpsdggflsk
     4501 msklpkkvlp hgskgeisag evsevsgevp velpearlkv gvkgperpds dvdisvsdkk
     4561 vtldvtkgfe gkvevstpdl gkpsdkgfis kvtkfpkkmf phgskggvsv gevsevtgdv
     4621 hvelpdahlk vgvkgperpd sdvdvsmavk kitvdvpegf egklevstpe larspdgslv
     4681 skaaklpkkl vpsgenslss veepslsahv dvpgsdttfk vdaklpampv tdvdislsak
     4741 essadipvgv agkveigtpd agkpsdkgfm skmakipkkm fhhgpkssgs tdehstvaet
     4801 vddkalgfev qlqkdvkipt kpeadvsvsf hekevtvqlp egregtvels tpglvgehvp
     4861 dkssqgadvl lpgkelptve gqeiiekpgg vievievtah elskplssgl lskvskipkk
     4921 ifprgsksks sdedspspgk relketpggi vevvevtkhd vaihpesthv kqvgtevlle
     4981 patsetkpsg aeglppthit reikevpggi vevvrvtkhi vgklpedkgi gepsvrttda
     5041 slpweaelak dpsavikrev ketpeghvev vqvirreasd vpfgsdfpgd lesdgtvsrr
     5101 iettvvdgkp tvirrlvttt sdgirqvteh ieqgaedags aasslfakmg efakglssdd
     5161 psavttrevk qlpggavevv svtkrssssp eteitsqeql qsstsgsmhf aesavgkpme
     5221 akttiirrei sgpetivrtt tsvvtsatap gsskpelsad vldkmfgame kprvddrlrd
     5281 vltaeggyva krevtpeeae llaqlfpeqs tgvavyppag lvpeaaiipe yrcmttaega
     5341 hhdpeskekv llklgralsq ddldapsptq geaamtvagl geavdtsdqv eepaveklel
     5401 arreskrysq efeqdeplpk amgfktkpse etvvsstqvk gvhdngtvkh tvttvtktti
     5461 iqeveepaga prpgesetwa sdldtsqvsv sslpederte ptlsssatta tadaealsgp
     5521 lartqveeke wtetdpdsge vrhiisktth ttvtskkpse gaprgdttst tvshsskdgh
     5581 hgstpsapse cdeaesltff qkkeffekls eqsppketss arlgstpsld taltrpavtl
     5641 kprprseslt sqgeiieesi vfaerlrlfq ngaqasplps asipqvdlvd papkmeqklq
     5701 gmaeigdslq qvpsavlegl qsteeksdtr llvhtsdadk eafdvrsvqv aedfgdkqfs
     5761 hmrvrmpape etvsktsics sdlpgdkpsp lrdaykgsls asrqessqag kpplpsalgg
     5821 dsslclvhea ttdltlskag ppnqtnkdit tfdrqpvreq sptlrssqsq mlasglqmgi
     5881 vagsgilqeg ikaspqsail etfsssatem tglespeirg lpseevhags aeeakgidag
     5941 rigavlaaap vailpvrdii sgkqlemldi aqvsdepnif egcvelpsqa esqtcpkekt
     6001 imkteyadiq hklssafges lapvesksgv tqgvpkevle aldrssqeli askldedser
     6061 dtnilsatqg siphdvfpdh iestldnsaa vpmrgaystp sstavcltpe rleiqatrev
     6121 aeerqekpev lvaleayrks tlrrsgedsy drnleefitv leeyeplpag qlsdwkrasp
     6181 afepsichns eitsklpple dkkeletvgs ildpsenvse llgnhaqsqm lcedtglltd
     6241 elgrtaghyv istaesdrge clptlseqap gkereeclny dsalgvrsts glessllees
     6301 heersldlar sedeqpslve dsmnvptess vidyqvvghs gvvkdaqtqp kdsdfdlhei
     6361 srtgdktdlp lscgddgkks aefsktvsgi aadmvikdtd sehlsqdmlh ptastvlceg
     6421 rsdvpyssde dqhkelsryk evelvipflr gtsssledev edggsfssgd lqaekiaefl
     6481 ttdpdtlnkv vhfpqifqdy dtsahqepfv ptetemnvek hvgfvldema acstpcvplk
     6541 sdrqteieea srivesvvhi asslveslhe vnhetaiked icdsalayaa gsplersevs
     6601 etlsqagpps tiekdqresp fgvkemyqvl kesrgsedri qysvtsfgdp lfadegdedq
     6661 errrrgspta eslddqaqie kcshevmmql ldgfsqsdve laevrtedea pdieddvpsp
     6721 eagsheeksv pssqdnsgdl qpqlstevei gsqasgapap lseldyelcp dadqlrtsgd
     6781 eavatrvppl eperdeavae sshapsaaad paasagrnag grerspllea sppeetaycp
     6841 eqdispaqey peyltpdmqv rvgvspteaq pakekpvrys essvesssev gspcppsqgg
     6901 tamafenvaf agedvidats spieessspa gsrspyevvr dahaeeaaqt alveaekslr
     6961 havqeevrar pqslvteess eqqehqiqps ifneafdhsp eesapekkql segllgvgll
     7021 tvsdttartp esrrgdsded eeewadhvms kirgrpippt ppaspravqs avevqgepwe
     7081 apvveqervn vesardmsae rdagvdvmgd rsatqpqlqe frrtadgkrp qlehipseis
     7141 dedlvereqs pthitatcrg drkdsssdrn sspdsdgpka sgshrrsasg vgaialeavh
     7201 sqdsggnekh shesrdkgaq eddkiteepv dtmfvieeea padsnrsesf cddaderaar
     7261 tagglspsle sppqkkdldd gsssgkpdaa spvvlrsksk rtdkisdkrs srdldtghsg
     7321 setdhshyys feltsdsgkt pgasrptsse fdvnilsgqa sseyetcats qdgevsgsya
     7381 taaasshdts fatarsslsg ssrssaysvd sessghlgsm evseasetiv asaheeldsd
     7441 iesersksvl repydgeipe svirggvehp flpswqpalk dssptlhgsa garpptaegv
     7501 fspfqstvps epgrscvgwp ssslstaesv rspskpevln gvsaplegqr rlrhnddfma
     7561 ssheeeqlsv sessatqseh twqtsvetmv daveaqrfsc dserhhrqst mqnsvslpfd
     7621 eavvaedshr eadddratpp fqevngpvev eyvpeyddlt artlrpvrqq napdgqteaq
     7681 gdvalleasa gdastsreqh eeenstelre egfyaetfqg dlsseevesa aneallldks
     7741 acsfyeycha kdeyevcntr pavesaslke aferpatpep vdeiddqede gnlaqgspnl
     7801 hgkdyeafgh asaeesvpcs dkthiaqels endqykvfsd sgnqsslhde trsksvsssl
     7861 eramtddvrl ytskcedyil qmhqtykygv glpktsdggy lpeegmdven vkllesrlqe
     7921 gnggqqdfpy dlsyqqeygg dgrvehgdly thqeveeegy ddersrtrgr rrlspisder
     7981 gstpdydtla grktfgkgse kddastsslm eferleaema sgtksssvgs sdslsgrqhq
     8041 grsgagseqd svsvnsltef erlekeivea egvdarlrge vaakldeide ghesqasdpg
     8101 hetavydesg atlllcasad vneepdgttd daqlgdieaa qgqevawsrt erhflttrte
     8161 sghsvesvdr qssastatqf dadslrerei ddsesnfgpd tpgwmqkecv dldslhdslh
     8221 dsehhdslqe vpskdkdslq lrepsadads lqedhnelqd slyetrdidw dsfradetsg
     8281 lddtsrswhq fpsqirttel sptlsrpass plvvpvvver idtltwvdqd pakacvsdge
     8341 slgsrqqehp rndalvsstd slepsssant hatyqcdtds mmsslnsaee stmvtstdtl
     8401 ehetlclasq gkslsleggl fsggdvteeq ntrtpleggv svddmeerfr ecllrsaasp
     8461 tneeqveerq tmdekgnliv trtvkrhvts eprlhtqtfm gpdaeqrsre fvasfaamep
     8521 saerdeyegf daagntvrvt qhvvvrptik tvtftgpdar lqmreymrsl aggqhssaen
     8581 tptsdsgvga csslaspppl mpdppaaaps sacpglqrls ggdqwmeaes alddcsppvt
     8641 atrvittrtt rtmgpdgreq lvtttteesg geepearlrr smqgvldsfm aepptpphld
     8701 eee
//
LOCUS       XP_077503798            8702 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X7 [Amblyomma
            americanum].
ACCESSION   XP_077503798
VERSION     XP_077503798.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647672.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8702
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..8702
                     /product="uncharacterized protein LOC144114148 isoform X7"
                     /calculated_mol_wt=934615
     Region          59..90
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          73..390
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(92,94,98..99,102..104,106..107,111,114,123,125,127,
                     131..132,135..137,139..140,144,147,156,158,160,164..165,
                     168..170,172..173,177,180)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          92..123
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          125..156
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          158..183
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          191..216
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          224..251
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(253,255,259..260,263..265,267..268,272,275,284,286,
                     288,292..293,296..298,300..301,305,308,317,319,321,
                     325..326,329..331,333..334,338,341)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          253..284
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          286..317
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          319..349
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          333..621
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          352..416
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          418..449
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(451,453,457..458,461..463,465..466,470,473,482,484,
                     486,490..491,494..496,498..499,503,506,515,517,519,
                     523..524,527..529,531..532,536,539)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          451..482
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          484..515
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          499..777
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          517..546
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          550..575
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          583..614
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          616..649
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          650..680
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          682..712
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(715,717,721..722,725..727,729..730,734,737,746,748,
                     750,754..755,758..760,762..763,767,770,779,781,783,
                     787..788,791..793,795..796,800,803)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          715..746
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          748..779
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          753..834
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          781..811
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          965..1068
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1291..1423
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1455..1538
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <1851..2102
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          <4465..4725
                     /region_name="YbbR"
                     /note="Cyclic di-AMP synthase regulator CdaR, YbbR domain
                     [Signal transduction mechanisms]; COG4856"
                     /db_xref="CDD:443884"
     Region          <7130..7443
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..8702
                     /gene="LOC144114148"
                     /coded_by="XM_077647672.1:883..26991"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 mgpgeptirq sreswigcli rllasqndqc dpsvylflrw ltllllravl sqmmppatdg
       61 nasflraara gnlekvldyl kgsidintsn anglnalhla akeghvnvvs ellkrganvn
      121 attkkgnsal hiaslagqee vvkllvqkqa nvnvqsqngf tplymaaqen hdgvvrflla
      181 nganqslate dgftplaval qqghdkvvav llendargkv rlpalhiask kddckaaall
      241 lhsehnpdvt sksgftplhi aahygnsnia sllldkgadv nfpakhqitp lhvaakwgks
      301 nmvklllekg akidastrdg ltplhcaars ghdqvveqll eknapitakt knglaplhma
      361 sqgdhvdsar illyhkapvd dvtvdyltal hvaahcghvg vakllldrra dpnaralngf
      421 tplhiackkn rikvvelllk hgasieatte sgltplhvas fmgcmniviy liqhganpdi
      481 ptvrgetplh laaranqtdi irillrngah vdakarelqt alhiasrlgn admvglllqh
      541 gaavdaptkd aytplhvaar egqdevaall ldhgaalaap tkkgftplhl aakygnlkva
      601 qlllqkdapv daqgkngvtp lhvaahydhv nvallllekg asphsaarng ytplhvaark
      661 dqmdiassll eygakpgaes ragftplhla aqeghadlaa llvehgadcd akakngltpm
      721 hlcaqedrve vatilakhga sldpttkagy tplhvachfg qtnmirfllr qganvnatts
      781 hgytplhqaa qqghtliinl llehraapna vtnqgqtala iaqrlgyisv vetlkvvtet
      841 ivttttttvt eekyrvvape tmhetfmsds edeaaednml gdqsfrylta demkslgdds
      901 lpidvtrder itesihitre pghpapltqe eerlsptqah tteaifvgny apdnvdlsrt
      961 pihagflvsf lvdarggamr gcrhsgvrvi ipprkaqmpm ritcrylrke klphppplle
     1021 geacasrile vgpagakflg pvilevphfa slrgkereis ilrsdngetw kehtleasee
     1081 avqevlnesf egeelsaled lqtnrivril ttdfpqyfav vsrtrqevha vgpeggllss
     1141 tvvpqvqaif pegaltkkir vglqaqpipa elvtkllghr vavspivtve prrrkfhkpi
     1201 tltipvpqaa tkgminqyag daptlrllcs itggtnkaqw edvtgstplt fvndcvsftt
     1261 tvsarfwlmd crqvseatkf atelyaeamh vpfmakfvvf akrqdpmeaq lrvfcmtddk
     1321 edktlesqeh ftevaksrdv evlekksqyl efggnlvpvt ksgeqlqlqf qafrenrlpf
     1381 avrvkdphqe plgrlafmrq praaratepp qaplcnlnia lpeyeasqnl selvtlekky
     1441 gfveetglak pelihradlr lsdiarelgs dwpalaaqld vpeqdvasir tecpsdlaqq
     1501 allmlrlwmk raggratgnn lekglrminr edivnkcmfn velvtddvek avakvhldqs
     1561 gfdtfreelg tpkdtslkrd asldvsydeq dlmkaesaee tssetgsvhe khtgseakgt
     1621 skaipgvrkg spisaaigri vrgsskekaa prmaveetfe eepvlkidsa evtqqaapea
     1681 gptapprdsk kkskkeksps plgpdyslpr mrdyhtperd ftsttdtliv ssikedvdas
     1741 kelatdgkvk kekkkekkek spsppgpdyt lprlrdtytp psdvtaagya astlpgqgit
     1801 leqgivkepl erkskersls pgvvktgeng slgadpsver qvkqlsqgpe dtiyekverv
     1861 vketpgkive mvhikrqrss kspegrgspk dknkglpsse vvapdisges rsvpsetiga
     1921 ekkqkkhket sptkqgkkea kevseavdkd aelsasdgkl pdsgflskma klpkkmfprs
     1981 gkasasdeep sslsesspva kkkagklpks atlpkakpsk tplyeiksvd vevdtsklts
     2041 kgvkgvpakd gegletsrdk epdsrffskm aripkkmfpr ssktsssede pssasenlps
     2101 skqrakkeke isskkkekvv kvkpdskkls tsfevevdrd tlpgagvdin akkpglqesl
     2161 esesrkktds mqisfpqsvk avgskgepme ysldiegmks lqkepqlavv gefpgkpspd
     2221 ielsltsket apefsdkarg hieigtpehg ksskggfmtk maklprkmfp hgfkgdvsve
     2281 epsveaesvh vsvpeaqvkl gtkdlkkkgp dmpameltas lpvgaegsve msapdlpvsa
     2341 dggliskvtq fpkmfshdsq dgvtierppv vgegidvess lpdvqlkvkg kafekpetkf
     2401 dvtlperkvt pvlpdgaegh ievnldemgk syddgfmskm aklpkkmfsh gledeiavqe
     2461 psitqhdihv dipklkdele iratlpekpq vdfdvaipgk qvsldlpegv eskievsapd
     2521 vgkptdggfm skvvqlpkkm fphgskgtvp leeapeavgd ihakvpeahl kvglkglekp
     2581 dfegdiavpg kkgavslpeg tegkievstp etgkpsdkgf mskmaklpkk mfphgskgsv
     2641 sveevpevdr svkvkvpqgk ipeahlkvdi kdpekhkdvd ialseekvdi dlpegidkni
     2701 evtmpevskp seggfiskma kfpkkmfphg akgsvsveea pgidvdvkmp etrismdvkg
     2761 lekpkgevdi slpkekvdid vpkgfdgkle msapevgkps eggfmskmak fpkkmfphga
     2821 kgsvsveeap gidvdvkvpe ahismdvkgl ekprgevdis lpkekvdidv pkgfdgklem
     2881 sapevgkpse ggfmskmakf pkkmfphgak gslsveetpg idvdvkmpea dlsmdvkgle
     2941 kpkaeldiam pkgkididmp egvdgkleas apdigkpsdg gfiskmtkfp kkmfphgakg
     3001 tvsveapelg dvqvnmpeah lgvdikgpek pkgeldvtlp kgkvdidlpe giegkveisa
     3061 peigkpdggf mskmaklpkk mfphgakggv svegspelgd vevkmpeahl gvdikgpekp
     3121 kgeldvtlpk gkvdidlpeg iegkveisap eigkpdggfm skmaklpkkm fphgakggvs
     3181 vegspelgdv evkmpeahlg vdikgpekpk geldvtlpkg kvdidlpegi egkveisape
     3241 igkpdggfms kmaklpkkmf phgakggvsv egspelgdve vkmpeahlgv dikgpekpkg
     3301 eldvtlpkgk vdidlpegie gkveisapei gkpdggfmsk maklpkkmfs hgakggvsve
     3361 gspelgdvqv kmpeahlgvd ikgpekpkge ldvtlpkgkv didlpegieg kveisspeig
     3421 kpdggfmskm aklpkkmfph gakggvsveg apelgdvevk mpeahlgvdi kgpekpkgel
     3481 dvtlpkgkvd idlpegiegk veisapeigk pdggfmskma klpkkmfphg akggvsvega
     3541 pelgdvevkm peahlgvdik gpekpkgeld vtlpkgkvdi dlpegfegkv eisapeigkp
     3601 dggfmskmak lpkkmfphgt kggvsvegap elgdvevkmp eahlgvdikg pekpkgeldv
     3661 tlpkgkvdid lpegiegkve isspeigkpd ggfmskmakl pkkmfphgak ggvsvegape
     3721 lgdvevkmpe ahlgvdikgp ekpkgeldvt lpkgkvdidl pegiegkvei sapeigkpdg
     3781 gfmskmaklp kkmfphgakg gvsvegspel gdvevkmpea hlgvdikgpe kpkgeldvtl
     3841 pkgkvdidlp egiegkleis apeigkpdgg fmskmakfpk kmfphgakgg vsvegapelg
     3901 dvqvkmpeah lgvdikgpek pkgeldvtlp kgkvdidlpe giegkveisa peigkpdggf
     3961 mskmakfpkk mfphgakggv svegapelgd vqvkmpeahl gvdiegpekp kgeldvtlpk
     4021 gkvdidlpeg iegkveisap eigkpdggfm skmakfpkkm fphgtkggvs vegapelgdv
     4081 evkmpeahlg vdikgpekpk geldvtlpkg kvdidlpegi egkveisape igkpdggfms
     4141 kmaklpqkmf phgtkggvsv egapelgdiq lkmpeahlgv dikgpekpkg eldamlpkgk
     4201 vdidlpegie gkveisapei gkpdggfmsk maklpkkmfs hgakgsisve gspefgdvev
     4261 kmpeahlggd ikgpekpkge ldvalpkgkv didipegieg kveisapeig kpsdggfvsk
     4321 maklpkkmfp hgtkgsvsie gapevgdvqv kvpeaqlkmd ikgtgkpefe geitwtgkkv
     4381 avelpegieg tvemstpemg rpsdggfmsk vaklpkkvfs hgskggvpfd eetgvagdie
     4441 vkmseaalkv dvkypekpes dfsalekkid idvpegfkgk veistpelgk psdggflskm
     4501 sklpkkvlph gskgeisage vsevsgevpv elpearlkvg vkgperpdsd vdisvsdkkv
     4561 tldvtkgfeg kvevstpdlg kpsdkgfisk vtkfpkkmfp hgskggvsvg evsevtgdvh
     4621 velpdahlkv gvkgperpds dvdvsmavkk itvdvpegfe gklevstpel arspdgslvs
     4681 kaaklpkklv psgenslssv eepslsahvd vpgsdttfkv daklpampvt dvdislsake
     4741 ssadipvgva gkveigtpda gkpsdkgfms kmakipkkmf hhgpkssgst dehstvaetv
     4801 ddkalgfevq lqkdvkiptk peadvsvsfh ekevtvqlpe gregtvelst pglvgehvpd
     4861 kssqgadvll pgkelptveg qeiiekpggv ievievtahe lskplssgll skvskipkki
     4921 fprgskskss dedspspgkr elketpggiv evvevtkhdv aihpesthvk qvgtevllep
     4981 atsetkpsga eglppthitr eikevpggiv evvrvtkhiv gklpedkgig epsvrttdas
     5041 lpweaelakd psavikrevk etpeghvevv qvirreasdv pfgsdfpgdl esdgtvsrri
     5101 ettvvdgkpt virrlvttts dgirqvtehi eqgaedagsa asslfakmge fakglssddp
     5161 savttrevkq lpggavevvs vtkrsssspe teitsqeqlq sstsgsmhfa esavgkpmea
     5221 kttiirreis gpetivrttt svvtsatapg sskpelsadv ldkmfgamek prvddrlrdv
     5281 ltaeggyvak revtpeeael laqlfpeqst gvavyppagl vpeaaiipey rcmttaegah
     5341 hdpeskekvl lklgralsqd dldapsptqg eaamtvaglg eavdtsdqve epaveklela
     5401 rreskrysqe feqdeplpka mgfktkpsee tvvsstqvkg vhdngtvkht vttvtkttii
     5461 qeveepagap rpgesetwas dldtsqvsvs slpedertep tlsssattat adaealsgpl
     5521 artqveekew tetdpdsgev rhiiskttht tvtskkpseg aprgdttstt vshsskdghh
     5581 gstpsapsec deaesltffq kkeffeklse qsppketssa rlgstpsldt altrpavtlk
     5641 prprseslts qgeiieesiv faerlrlfqn gaqasplpsa sipqvdlvdp apkmeqklqg
     5701 maeigdslqq vpsavleglq steeksdtrl lvhtsdadke afdvrsvqva edfgdkqfsh
     5761 mrvrmpapee tvsktsicss dlpgdkpspl rdaykgslsa srqessqagk pplpsalggd
     5821 sslclvheat tdltlskagp pnqtnkditt fdrqpvreqs ptlrssqsqm lasglqmgiv
     5881 agsgilqegi kaspqsaile tfsssatemt glespeirgl pseevhagsa eeakgidagr
     5941 igavlaaapv ailpvrdiis gkqlemldia qvsdepnife gcvelpsqae sqtcpkekti
     6001 mkteyadiqh klssafgesl apvesksgvt qgvpkevlea ldrssqelia skldedserd
     6061 tnilsatqgs iphdvfpdhi estldnsaav pmrgaystps stavcltper leiqatreva
     6121 eerqekpevl valeayrkst lrrsgedsyd rnleefitvl eeyeplpagq lsdwkraspa
     6181 fepsichnse itsklppled kkeletvgsi ldpsenvsel lgnhaqsqml cedtglltde
     6241 lgrtaghyvi staesdrgec lptlseqapg kereeclnyd salgvrstsg lesslleesh
     6301 eersldlars edeqpslved smnvptessv idyqvvghsg vvkdaqtqpk dsdfdlheis
     6361 rtgdktdlpl scgddgkksa efsktvsgia admvikdtds ehlsqdmlhp tastvlcegr
     6421 sdvpyssded qhkelsryke velvipflrg tsssledeve dggsfssgdl qaekiaeflt
     6481 tdpdtlnkvv hfpqifqdyd tsahqepfvp tetemnvekh vgfvldemaa cstpcvplks
     6541 drqteieeas rivesvvhia sslveslhev nhetaikedi cdsalayaag splersevse
     6601 tlsqagppst iekdqrespf gvkemyqvlk esrgsedriq ysvtsfgdpl fadegdedqe
     6661 rrrrgsptae slddqaqiek cshevmmqll dgfsqsdvel aevrtedeap dieddvpspe
     6721 agsheeksvp ssqdnsgdlq pqlsteveig sqasgapapl seldyelcpd adqlrtsgde
     6781 avatrvpple perdeavaes shapsaaadp aasagrnagg rersplleas ppeetaycpe
     6841 qdispaqeyp eyltpdmqvr vgvspteaqp akekpvryse ssvesssevg spcppsqggt
     6901 amafenvafa gedvidatss pieessspag srspyevvrd ahaeeaaqta lveaekslrh
     6961 avqeevrarp qslvteesse qqehqiqpsi fneafdhspe esapekkqls egllgvgllt
     7021 vsdttartpe srrgdsdede eewadhvmsk irgrpipptp paspravqsa vevqgepwea
     7081 pvveqervnv esardmsaer dagvdvmgdr satqpqlqef rrtadgkrpq lehipseisd
     7141 edlvereqsp thitatcrgd rkdsssdrns spdsdgpkas gshrrsasgv gaialeavhs
     7201 qdsggnekhs hesrdkgaqe ddkiteepvd tmfvieeeap adsnrsesfc ddaderaart
     7261 agglspsles ppqkkdlddg sssgkpdaas pvvlrskskr tdkisdkrss rdldtghsgs
     7321 etdhshyysf eltsdsgktp gasrptssef dvnilsgqas seyetcatsq dgevsgsyat
     7381 aaasshdtsf atarsslsgs srssaysvds essghlgsme vseasetiva saheeldsdi
     7441 esersksvlr epydgeipes virggvehpf lpswqpalkd ssptlhgsag arpptaegvf
     7501 spfqstvpse pgrscvgwps sslstaesvr spskpevlng vsaplegqrr lrhnddfmas
     7561 sheeeqlsvs essatqseht wqtsvetmvd aveaqrfscd serhhrqstm qnsvslpfde
     7621 avvaedshre adddratppf qevngpveve yvpeyddlta rtlrpvrqqn apdgqteaqg
     7681 dvalleasag dastsreqhe eenstelree gfyaetfqgd lsseevesaa neallldksa
     7741 csfyeychak deyevcntrp avesaslkea ferpatpepv deiddqedeg nlaqgspnlh
     7801 gkdyeafgha saeesvpcsd kthiaqelse ndqykvfsds gnqsslhdet rsksvsssle
     7861 ramtddvrly tskcedyilq mhqtykygvg lpktsdggyl peegmdvenv kllesrlqeg
     7921 nggqqdfpyd lsyqqeyggd grvehgdlyt hqeveeegyd dersrtrgrr rlspisderg
     7981 stpdydtlag rktfgkgsek ddastsslme ferleaemas gtksssvgss dslsgrqhqg
     8041 rsgagseqds vsvnsltefe rlekeiveae gvdarlrgev aakldeideg hesqasdpgh
     8101 etavydesga tlllcasadv neepdgttdd aqlgdieaaq gqevawsrte rhflttrtes
     8161 ghsvesvdrq ssastatqfd adslrereid dsesnfgpdt pgwmqkecvd ldslhdslhd
     8221 sehhdslqev pskdkdslql repsadadsl qedhnelqds lyetrdidwd sfradetsgl
     8281 ddtsrswhqf psqirttels ptlsrpassp lvvpvvveri dtltwvdqdp akacvsdges
     8341 lgsrqqehpr ndalvsstds lepsssanth atyqcdtdsm msslnsaees tmvtstdtle
     8401 hetlclasqg kslslegglf sggdvteeqn trtpleggvs vddmeerfre cllrsaaspt
     8461 neeqveerqt mdekgnlivt rtvkrhvtse prlhtqtfmg pdaeqrsref vasfaameps
     8521 aerdeyegfd aagntvrvtq hvvvrptikt vtftgpdarl qmreymrsla ggqhssaent
     8581 ptsdsgvgac sslasppplm pdppaaapss acpglqrlsg gdqwmeaesa lddcsppvta
     8641 trvittrttr tmgpdgreql vtttteesgg eepearlrrs mqgvldsfma epptpphlde
     8701 ee
//
LOCUS       XP_077503799            8701 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X8 [Amblyomma
            americanum].
ACCESSION   XP_077503799
VERSION     XP_077503799.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647673.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8701
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..8701
                     /product="uncharacterized protein LOC144114148 isoform X8"
                     /calculated_mol_wt=934250
     Region          24..55
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          38..355
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(57,59,63..64,67..69,71..72,76,79,88,90,92,96..97,
                     100..102,104..105,109,112,121,123,125,129..130,133..135,
                     137..138,142,145)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          57..88
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          90..121
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          123..148
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          156..181
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          189..216
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(218,220,224..225,228..230,232..233,237,240,249,251,
                     253,257..258,261..263,265..266,270,273,282,284,286,
                     290..291,294..296,298..299,303,306)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          218..249
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          251..282
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          284..314
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          298..586
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          317..381
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          383..414
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(416,418,422..423,426..428,430..431,435,438,447,449,
                     451,455..456,459..461,463..464,468,471,480,482,484,
                     488..489,492..494,496..497,501,504)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          416..447
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          449..480
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          464..742
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          482..511
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          515..540
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          548..579
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          581..614
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          615..645
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          647..677
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(680,682,686..687,690..692,694..695,699,702,711,713,
                     715,719..720,723..725,727..728,732,735,744,746,748,
                     752..753,756..758,760..761,765,768)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          680..711
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          713..744
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          718..799
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          746..776
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          964..1066
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1289..1421
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1453..1536
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <1850..2101
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          <4464..4724
                     /region_name="YbbR"
                     /note="Cyclic di-AMP synthase regulator CdaR, YbbR domain
                     [Signal transduction mechanisms]; COG4856"
                     /db_xref="CDD:443884"
     Region          <7129..7442
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..8701
                     /gene="LOC144114148"
                     /coded_by="XM_077647673.1:253..26358"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 maitahdapp gspngqipds aqtdgnasfl raaragnlek vldylkgsid intsnangln
       61 alhlaakegh vnvvsellkr ganvnattkk gnsalhiasl agqeevvkll vqkqanvnvq
      121 sqngftplym aaqenhdgvv rfllanganq slatedgftp lavalqqghd kvvavllend
      181 argkvrlpal hiaskkddck aaalllhseh npdvtsksgf tplhiaahyg nsniasllld
      241 kgadvnfpak hqitplhvaa kwgksnmvkl llekgakida strdgltplh caarsghdqv
      301 veqlleknap itaktkngla plhmasqgdh vdsarillyh kapvddvtvd yltalhvaah
      361 cghvgvakll ldrradpnar alngftplhi ackknrikvv elllkhgasi eattesgltp
      421 lhvasfmgcm niviyliqhg anpdiptvrg etplhlaara nqtdiirill rngahvdaka
      481 relqtalhia srlgnadmvg lllqhgaavd aptkdaytpl hvaaregqde vaallldhga
      541 alaaptkkgf tplhlaakyg nlkvaqlllq kdapvdaqgk ngvtplhvaa hydhvnvall
      601 llekgasphs aarngytplh vaarkdqmdi asslleygak pgaesragft plhlaaqegh
      661 adlaallveh gadcdakakn gltpmhlcaq edrvevatil akhgasldpt tkagytplhv
      721 achfgqtnmi rfllrqganv nattshgytp lhqaaqqght liinlllehr aapnavtnqg
      781 qtalaiaqrl gyisvvetlk vvtetivttt tttvteekyr vvapetmhet fmsdsedeaa
      841 ednmlgdqsf ryltademks lgddslpidv trderitesi hitrepghpa pltqeeerls
      901 ptqahtteai fvgnyapdnv dlsrtpihag sllswdgdsc ssayvlsptk rmwressklk
      961 wktflvsflv darggamrgc rhsgvrviip prkaqmpmri tcrylrkekl phpppllege
     1021 acasrilevg pagakflgpv ilevphfasl rgkereisil rsdngetwke htleaseeav
     1081 qevlnesfeg eelsaledlq tnrivriltt dfpqyfavvs rtrqevhavg peggllsstv
     1141 vpqvqaifpe galtkkirvg lqaqpipael vtkllghrva vspivtvepr rrkfhkpitl
     1201 tipvpqaatk gminqyagda ptlrllcsit ggtnkaqwed vtgstpltfv ndcvsftttv
     1261 sarfwlmdcr qvseatkfat elyaeamhvp fmakfvvfak rqdpmeaqlr vfcmtddked
     1321 ktlesqehft evaksrdvev lekksqylef ggnlvpvtks geqlqlqfqa frenrlpfav
     1381 rvkdphqepl grlafmrqpr aarateppqa plcnlnialp eyeasqnlse lvtlekkygf
     1441 veetglakpe lihradlrls diarelgsdw palaaqldvp eqdvasirte cpsdlaqqal
     1501 lmlrlwmkra ggratgnnle kglrminred ivnkcmfnve lvtddvekav akvhldqsgf
     1561 dtfreelgtp kdtslkrdas ldvsydeqdl mkeaesaeet ssetgsvhek htgseakgts
     1621 kaipgvrkgs pisaaigriv rgsskekaap rmaveetfee epvlkidsae vtqqaapeag
     1681 ptapprdskk kskkekspsp lgpdyslprm rdyhtperdf tsttdtlivs sikedvdask
     1741 elatdgkvkk ekkkekkeks psppgpdytl prlrdtytpp sdvtaagyaa stlpgqgitl
     1801 eqgivkeple rkskerslsp gvvktgengs lgadpsverq vkqlsqgped tiyekvervv
     1861 ketpgkivem vhikrqrssk spegrgspkd knkglpssev vapdisgesr svpsetigae
     1921 kkqkkhkets ptkqgkkeak evseavdkda elsasdgklp dsgflskmak lpkkmfprsg
     1981 kasasdeeps slsesspvak kkagklpksa tlpkakpskt plyeiksvdv evdtskltsk
     2041 gvkgvpakdg egletsrdke pdsrffskma ripkkmfprs sktsssedep ssasenlpss
     2101 kqrakkekei sskkkekvvk vkpdskklst sfevevdrdt lpgagvdina kkpglqesle
     2161 sesrkktdsm qisfpqsvka vgskgepmey sldiegmksl qkepqlavvg efpgkpspdi
     2221 elsltsketa pefsdkargh ieigtpehgk sskggfmtkm aklprkmfph gfkgdvsvee
     2281 psveaesvhv svpeaqvklg tkdlkkkgpd mpameltasl pvgaegsvem sapdlpvsad
     2341 ggliskvtqf pkmfshdsqd gvtierppvv gegidvessl pdvqlkvkgk afekpetkfd
     2401 vtlperkvtp vlpdgaeghi evnldemgks yddgfmskma klpkkmfshg ledeiavqep
     2461 sitqhdihvd ipklkdelei ratlpekpqv dfdvaipgkq vsldlpegve skievsapdv
     2521 gkptdggfms kvvqlpkkmf phgskgtvpl eeapeavgdi hakvpeahlk vglkglekpd
     2581 fegdiavpgk kgavslpegt egkievstpe tgkpsdkgfm skmaklpkkm fphgskgsvs
     2641 veevpevdrs vkvkvpqgki peahlkvdik dpekhkdvdi alseekvdid lpegidknie
     2701 vtmpevskps eggfiskmak fpkkmfphga kgsvsveeap gidvdvkmpe trismdvkgl
     2761 ekpkgevdis lpkekvdidv pkgfdgklem sapevgkpse ggfmskmakf pkkmfphgak
     2821 gsvsveeapg idvdvkvpea hismdvkgle kprgevdisl pkekvdidvp kgfdgklems
     2881 apevgkpseg gfmskmakfp kkmfphgakg slsveetpgi dvdvkmpead lsmdvkglek
     2941 pkaeldiamp kgkididmpe gvdgkleasa pdigkpsdgg fiskmtkfpk kmfphgakgt
     3001 vsveapelgd vqvnmpeahl gvdikgpekp kgeldvtlpk gkvdidlpeg iegkveisap
     3061 eigkpdggfm skmaklpkkm fphgakggvs vegspelgdv evkmpeahlg vdikgpekpk
     3121 geldvtlpkg kvdidlpegi egkveisape igkpdggfms kmaklpkkmf phgakggvsv
     3181 egspelgdve vkmpeahlgv dikgpekpkg eldvtlpkgk vdidlpegie gkveisapei
     3241 gkpdggfmsk maklpkkmfp hgakggvsve gspelgdvev kmpeahlgvd ikgpekpkge
     3301 ldvtlpkgkv didlpegieg kveisapeig kpdggfmskm aklpkkmfsh gakggvsveg
     3361 spelgdvqvk mpeahlgvdi kgpekpkgel dvtlpkgkvd idlpegiegk veisspeigk
     3421 pdggfmskma klpkkmfphg akggvsvega pelgdvevkm peahlgvdik gpekpkgeld
     3481 vtlpkgkvdi dlpegiegkv eisapeigkp dggfmskmak lpkkmfphga kggvsvegap
     3541 elgdvevkmp eahlgvdikg pekpkgeldv tlpkgkvdid lpegfegkve isapeigkpd
     3601 ggfmskmakl pkkmfphgtk ggvsvegape lgdvevkmpe ahlgvdikgp ekpkgeldvt
     3661 lpkgkvdidl pegiegkvei sspeigkpdg gfmskmaklp kkmfphgakg gvsvegapel
     3721 gdvevkmpea hlgvdikgpe kpkgeldvtl pkgkvdidlp egiegkveis apeigkpdgg
     3781 fmskmaklpk kmfphgakgg vsvegspelg dvevkmpeah lgvdikgpek pkgeldvtlp
     3841 kgkvdidlpe giegkleisa peigkpdggf mskmakfpkk mfphgakggv svegapelgd
     3901 vqvkmpeahl gvdikgpekp kgeldvtlpk gkvdidlpeg iegkveisap eigkpdggfm
     3961 skmakfpkkm fphgakggvs vegapelgdv qvkmpeahlg vdiegpekpk geldvtlpkg
     4021 kvdidlpegi egkveisape igkpdggfms kmakfpkkmf phgtkggvsv egapelgdve
     4081 vkmpeahlgv dikgpekpkg eldvtlpkgk vdidlpegie gkveisapei gkpdggfmsk
     4141 maklpqkmfp hgtkggvsve gapelgdiql kmpeahlgvd ikgpekpkge ldamlpkgkv
     4201 didlpegieg kveisapeig kpdggfmskm aklpkkmfsh gakgsisveg spefgdvevk
     4261 mpeahlggdi kgpekpkgel dvalpkgkvd idipegiegk veisapeigk psdggfvskm
     4321 aklpkkmfph gtkgsvsieg apevgdvqvk vpeaqlkmdi kgtgkpefeg eitwtgkkva
     4381 velpegiegt vemstpemgr psdggfmskv aklpkkvfsh gskggvpfde etgvagdiev
     4441 kmseaalkvd vkypekpesd fsalekkidi dvpegfkgkv eistpelgkp sdggflskms
     4501 klpkkvlphg skgeisagev sevsgevpve lpearlkvgv kgperpdsdv disvsdkkvt
     4561 ldvtkgfegk vevstpdlgk psdkgfiskv tkfpkkmfph gskggvsvge vsevtgdvhv
     4621 elpdahlkvg vkgperpdsd vdvsmavkki tvdvpegfeg klevstpela rspdgslvsk
     4681 aaklpkklvp sgenslssve epslsahvdv pgsdttfkvd aklpampvtd vdislsakes
     4741 sadipvgvag kveigtpdag kpsdkgfmsk makipkkmfh hgpkssgstd ehstvaetvd
     4801 dkalgfevql qkdvkiptkp eadvsvsfhe kevtvqlpeg regtvelstp glvgehvpdk
     4861 ssqgadvllp gkelptvegq eiiekpggvi evievtahel skplssglls kvskipkkif
     4921 prgskskssd edspspgkre lketpggive vvevtkhdva ihpesthvkq vgtevllepa
     4981 tsetkpsgae glppthitre ikevpggive vvrvtkhivg klpedkgige psvrttdasl
     5041 pweaelakdp savikrevke tpeghvevvq virreasdvp fgsdfpgdle sdgtvsrrie
     5101 ttvvdgkptv irrlvtttsd girqvtehie qgaedagsaa sslfakmgef akglssddps
     5161 avttrevkql pggavevvsv tkrsssspet eitsqeqlqs stsgsmhfae savgkpmeak
     5221 ttiirreisg petivrttts vvtsatapgs skpelsadvl dkmfgamekp rvddrlrdvl
     5281 taeggyvakr evtpeeaell aqlfpeqstg vavyppaglv peaaiipeyr cmttaegahh
     5341 dpeskekvll klgralsqdd ldapsptqge aamtvaglge avdtsdqvee paveklelar
     5401 reskrysqef eqdeplpkam gfktkpseet vvsstqvkgv hdngtvkhtv ttvtkttiiq
     5461 eveepagapr pgesetwasd ldtsqvsvss lpedertept lsssattata daealsgpla
     5521 rtqveekewt etdpdsgevr hiisktthtt vtskkpsega prgdttsttv shsskdghhg
     5581 stpsapsecd eaesltffqk keffeklseq sppketssar lgstpsldta ltrpavtlkp
     5641 rprsesltsq geiieesivf aerlrlfqng aqasplpsas ipqvdlvdpa pkmeqklqgm
     5701 aeigdslqqv psavleglqs teeksdtrll vhtsdadkea fdvrsvqvae dfgdkqfshm
     5761 rvrmpapeet vsktsicssd lpgdkpsplr daykgslsas rqessqagkp plpsalggds
     5821 slclvheatt dltlskagpp nqtnkdittf drqpvreqsp tlrssqsqml asglqmgiva
     5881 gsgilqegik aspqsailet fsssatemtg lespeirglp seevhagsae eakgidagri
     5941 gavlaaapva ilpvrdiisg kqlemldiaq vsdepnifeg cvelpsqaes qtcpkektim
     6001 kteyadiqhk lssafgesla pvesksgvtq gvpkevleal drssqelias kldedserdt
     6061 nilsatqgsi phdvfpdhie stldnsaavp mrgaystpss tavcltperl eiqatrevae
     6121 erqekpevlv aleayrkstl rrsgedsydr nleefitvle eyeplpagql sdwkraspaf
     6181 epsichnsei tsklppledk keletvgsil dpsenvsell gnhaqsqmlc edtglltdel
     6241 grtaghyvis taesdrgecl ptlseqapgk ereeclnyds algvrstsgl esslleeshe
     6301 ersldlarse deqpslveds mnvptessvi dyqvvghsgv vkdaqtqpkd sdfdlheisr
     6361 tgdktdlpls cgddgkksae fsktvsgiaa dmvikdtdse hlsqdmlhpt astvlcegrs
     6421 dvpyssdedq hkelsrykev elvipflrgt sssledeved ggsfssgdlq aekiaefltt
     6481 dpdtlnkvvh fpqifqdydt sahqepfvpt etemnvekhv gfvldemaac stpcvplksd
     6541 rqteieeasr ivesvvhias slveslhevn hetaikedic dsalayaags plersevset
     6601 lsqagppsti ekdqrespfg vkemyqvlke srgsedriqy svtsfgdplf adegdedqer
     6661 rrrgsptaes lddqaqiekc shevmmqlld gfsqsdvela evrtedeapd ieddvpspea
     6721 gsheeksvps sqdnsgdlqp qlsteveigs qasgapapls eldyelcpda dqlrtsgdea
     6781 vatrvpplep erdeavaess hapsaaadpa asagrnaggr ersplleasp peetaycpeq
     6841 dispaqeype yltpdmqvrv gvspteaqpa kekpvryses svesssevgs pcppsqggta
     6901 mafenvafag edvidatssp ieessspags rspyevvrda haeeaaqtal veaekslrha
     6961 vqeevrarpq slvteesseq qehqiqpsif neafdhspee sapekkqlse gllgvglltv
     7021 sdttartpes rrgdsdedee ewadhvmski rgrpipptpp aspravqsav evqgepweap
     7081 vveqervnve sardmsaerd agvdvmgdrs atqpqlqefr rtadgkrpql ehipseisde
     7141 dlvereqspt hitatcrgdr kdsssdrnss pdsdgpkasg shrrsasgvg aialeavhsq
     7201 dsggnekhsh esrdkgaqed dkiteepvdt mfvieeeapa dsnrsesfcd daderaarta
     7261 gglspslesp pqkkdlddgs ssgkpdaasp vvlrskskrt dkisdkrssr dldtghsgse
     7321 tdhshyysfe ltsdsgktpg asrptssefd vnilsgqass eyetcatsqd gevsgsyata
     7381 aasshdtsfa tarsslsgss rssaysvdse ssghlgsmev seasetivas aheeldsdie
     7441 sersksvlre pydgeipesv irggvehpfl pswqpalkds sptlhgsaga rpptaegvfs
     7501 pfqstvpsep grscvgwpss slstaesvrs pskpevlngv saplegqrrl rhnddfmass
     7561 heeeqlsvse ssatqsehtw qtsvetmvda veaqrfscds erhhrqstmq nsvslpfdea
     7621 vvaedshrea dddratppfq evngpvevey vpeyddltar tlrpvrqqna pdgqteaqgd
     7681 valleasagd astsreqhee enstelreeg fyaetfqgdl sseevesaan eallldksac
     7741 sfyeychakd eyevcntrpa vesaslkeaf erpatpepvd eiddqedegn laqgspnlhg
     7801 kdyeafghas aeesvpcsdk thiaqelsen dqykvfsdsg nqsslhdetr sksvsssler
     7861 amtddvrlyt skcedyilqm hqtykygvgl pktsdggylp eegmdvenvk llesrlqegn
     7921 ggqqdfpydl syqqeyggdg rvehgdlyth qeveeegydd ersrtrgrrr lspisdergs
     7981 tpdydtlagr ktfgkgsekd dastsslmef erleaemasg tksssvgssd slsgrqhqgr
     8041 sgagseqdsv svnsltefer lekeiveaeg vdarlrgeva akldeidegh esqasdpghe
     8101 tavydesgat lllcasadvn eepdgttdda qlgdieaaqg qevawsrter hflttrtesg
     8161 hsvesvdrqs sastatqfda dslrereidd sesnfgpdtp gwmqkecvdl dslhdslhds
     8221 ehhdslqevp skdkdslqlr epsadadslq edhnelqdsl yetrdidwds fradetsgld
     8281 dtsrswhqfp sqirttelsp tlsrpasspl vvpvvverid tltwvdqdpa kacvsdgesl
     8341 gsrqqehprn dalvsstdsl epsssantha tyqcdtdsmm sslnsaeest mvtstdtleh
     8401 etlclasqgk slslegglfs ggdvteeqnt rtpleggvsv ddmeerfrec llrsaasptn
     8461 eeqveerqtm dekgnlivtr tvkrhvtsep rlhtqtfmgp daeqrsrefv asfaamepsa
     8521 erdeyegfda agntvrvtqh vvvrptiktv tftgpdarlq mreymrslag gqhssaentp
     8581 tsdsgvgacs slasppplmp dppaaapssa cpglqrlsgg dqwmeaesal ddcsppvtat
     8641 rvittrttrt mgpdgreqlv tttteesgge epearlrrsm qgvldsfmae pptpphldee
     8701 e
//
LOCUS       XP_077503800            8700 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X9 [Amblyomma
            americanum].
ACCESSION   XP_077503800
VERSION     XP_077503800.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647674.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8700
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..8700
                     /product="uncharacterized protein LOC144114148 isoform X9"
                     /calculated_mol_wt=934120
     Region          24..55
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          38..355
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(57,59,63..64,67..69,71..72,76,79,88,90,92,96..97,
                     100..102,104..105,109,112,121,123,125,129..130,133..135,
                     137..138,142,145)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          57..88
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          90..121
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          123..148
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          156..181
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          189..216
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(218,220,224..225,228..230,232..233,237,240,249,251,
                     253,257..258,261..263,265..266,270,273,282,284,286,
                     290..291,294..296,298..299,303,306)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          218..249
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          251..282
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          284..314
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          298..586
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          317..381
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          383..414
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(416,418,422..423,426..428,430..431,435,438,447,449,
                     451,455..456,459..461,463..464,468,471,480,482,484,
                     488..489,492..494,496..497,501,504)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          416..447
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          449..480
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          464..742
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          482..511
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          515..540
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          548..579
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          581..614
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          615..645
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          647..677
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(680,682,686..687,690..692,694..695,699,702,711,713,
                     715,719..720,723..725,727..728,732,735,744,746,748,
                     752..753,756..758,760..761,765,768)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          680..711
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          713..744
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          718..799
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          746..776
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          964..1066
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1289..1421
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1453..1536
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <1849..2100
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          <4463..4723
                     /region_name="YbbR"
                     /note="Cyclic di-AMP synthase regulator CdaR, YbbR domain
                     [Signal transduction mechanisms]; COG4856"
                     /db_xref="CDD:443884"
     Region          <7128..7441
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..8700
                     /gene="LOC144114148"
                     /coded_by="XM_077647674.1:264..26366"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 maitahdapp gspngqipds aqtdgnasfl raaragnlek vldylkgsid intsnangln
       61 alhlaakegh vnvvsellkr ganvnattkk gnsalhiasl agqeevvkll vqkqanvnvq
      121 sqngftplym aaqenhdgvv rfllanganq slatedgftp lavalqqghd kvvavllend
      181 argkvrlpal hiaskkddck aaalllhseh npdvtsksgf tplhiaahyg nsniasllld
      241 kgadvnfpak hqitplhvaa kwgksnmvkl llekgakida strdgltplh caarsghdqv
      301 veqlleknap itaktkngla plhmasqgdh vdsarillyh kapvddvtvd yltalhvaah
      361 cghvgvakll ldrradpnar alngftplhi ackknrikvv elllkhgasi eattesgltp
      421 lhvasfmgcm niviyliqhg anpdiptvrg etplhlaara nqtdiirill rngahvdaka
      481 relqtalhia srlgnadmvg lllqhgaavd aptkdaytpl hvaaregqde vaallldhga
      541 alaaptkkgf tplhlaakyg nlkvaqlllq kdapvdaqgk ngvtplhvaa hydhvnvall
      601 llekgasphs aarngytplh vaarkdqmdi asslleygak pgaesragft plhlaaqegh
      661 adlaallveh gadcdakakn gltpmhlcaq edrvevatil akhgasldpt tkagytplhv
      721 achfgqtnmi rfllrqganv nattshgytp lhqaaqqght liinlllehr aapnavtnqg
      781 qtalaiaqrl gyisvvetlk vvtetivttt tttvteekyr vvapetmhet fmsdsedeaa
      841 ednmlgdqsf ryltademks lgddslpidv trderitesi hitrepghpa pltqeeerls
      901 ptqahtteai fvgnyapdnv dlsrtpihag sllswdgdsc ssayvlsptk rmwressklk
      961 wktflvsflv darggamrgc rhsgvrviip prkaqmpmri tcrylrkekl phpppllege
     1021 acasrilevg pagakflgpv ilevphfasl rgkereisil rsdngetwke htleaseeav
     1081 qevlnesfeg eelsaledlq tnrivriltt dfpqyfavvs rtrqevhavg peggllsstv
     1141 vpqvqaifpe galtkkirvg lqaqpipael vtkllghrva vspivtvepr rrkfhkpitl
     1201 tipvpqaatk gminqyagda ptlrllcsit ggtnkaqwed vtgstpltfv ndcvsftttv
     1261 sarfwlmdcr qvseatkfat elyaeamhvp fmakfvvfak rqdpmeaqlr vfcmtddked
     1321 ktlesqehft evaksrdvev lekksqylef ggnlvpvtks geqlqlqfqa frenrlpfav
     1381 rvkdphqepl grlafmrqpr aarateppqa plcnlnialp eyeasqnlse lvtlekkygf
     1441 veetglakpe lihradlrls diarelgsdw palaaqldvp eqdvasirte cpsdlaqqal
     1501 lmlrlwmkra ggratgnnle kglrminred ivnkcmfnve lvtddvekav akvhldqsgf
     1561 dtfreelgtp kdtslkrdas ldvsydeqdl mkaesaeets setgsvhekh tgseakgtsk
     1621 aipgvrkgsp isaaigrivr gsskekaapr maveetfeee pvlkidsaev tqqaapeagp
     1681 tapprdskkk skkekspspl gpdyslprmr dyhtperdft sttdtlivss ikedvdaske
     1741 latdgkvkke kkkekkeksp sppgpdytlp rlrdtytpps dvtaagyaas tlpgqgitle
     1801 qgivkepler kskerslspg vvktgengsl gadpsverqv kqlsqgpedt iyekvervvk
     1861 etpgkivemv hikrqrssks pegrgspkdk nkglpssevv apdisgesrs vpsetigaek
     1921 kqkkhketsp tkqgkkeake vseavdkdae lsasdgklpd sgflskmakl pkkmfprsgk
     1981 asasdeepss lsesspvakk kagklpksat lpkakpsktp lyeiksvdve vdtskltskg
     2041 vkgvpakdge gletsrdkep dsrffskmar ipkkmfprss ktsssedeps sasenlpssk
     2101 qrakkekeis skkkekvvkv kpdskklsts fevevdrdtl pgagvdinak kpglqesles
     2161 esrkktdsmq isfpqsvkav gskgepmeys ldiegmkslq kepqlavvge fpgkpspdie
     2221 lsltsketap efsdkarghi eigtpehgks skggfmtkma klprkmfphg fkgdvsveep
     2281 sveaesvhvs vpeaqvklgt kdlkkkgpdm pameltaslp vgaegsvems apdlpvsadg
     2341 gliskvtqfp kmfshdsqdg vtierppvvg egidvesslp dvqlkvkgka fekpetkfdv
     2401 tlperkvtpv lpdgaeghie vnldemgksy ddgfmskmak lpkkmfshgl edeiavqeps
     2461 itqhdihvdi pklkdeleir atlpekpqvd fdvaipgkqv sldlpegves kievsapdvg
     2521 kptdggfmsk vvqlpkkmfp hgskgtvple eapeavgdih akvpeahlkv glkglekpdf
     2581 egdiavpgkk gavslpegte gkievstpet gkpsdkgfms kmaklpkkmf phgskgsvsv
     2641 eevpevdrsv kvkvpqgkip eahlkvdikd pekhkdvdia lseekvdidl pegidkniev
     2701 tmpevskpse ggfiskmakf pkkmfphgak gsvsveeapg idvdvkmpet rismdvkgle
     2761 kpkgevdisl pkekvdidvp kgfdgklems apevgkpseg gfmskmakfp kkmfphgakg
     2821 svsveeapgi dvdvkvpeah ismdvkglek prgevdislp kekvdidvpk gfdgklemsa
     2881 pevgkpsegg fmskmakfpk kmfphgakgs lsveetpgid vdvkmpeadl smdvkglekp
     2941 kaeldiampk gkididmpeg vdgkleasap digkpsdggf iskmtkfpkk mfphgakgtv
     3001 sveapelgdv qvnmpeahlg vdikgpekpk geldvtlpkg kvdidlpegi egkveisape
     3061 igkpdggfms kmaklpkkmf phgakggvsv egspelgdve vkmpeahlgv dikgpekpkg
     3121 eldvtlpkgk vdidlpegie gkveisapei gkpdggfmsk maklpkkmfp hgakggvsve
     3181 gspelgdvev kmpeahlgvd ikgpekpkge ldvtlpkgkv didlpegieg kveisapeig
     3241 kpdggfmskm aklpkkmfph gakggvsveg spelgdvevk mpeahlgvdi kgpekpkgel
     3301 dvtlpkgkvd idlpegiegk veisapeigk pdggfmskma klpkkmfshg akggvsvegs
     3361 pelgdvqvkm peahlgvdik gpekpkgeld vtlpkgkvdi dlpegiegkv eisspeigkp
     3421 dggfmskmak lpkkmfphga kggvsvegap elgdvevkmp eahlgvdikg pekpkgeldv
     3481 tlpkgkvdid lpegiegkve isapeigkpd ggfmskmakl pkkmfphgak ggvsvegape
     3541 lgdvevkmpe ahlgvdikgp ekpkgeldvt lpkgkvdidl pegfegkvei sapeigkpdg
     3601 gfmskmaklp kkmfphgtkg gvsvegapel gdvevkmpea hlgvdikgpe kpkgeldvtl
     3661 pkgkvdidlp egiegkveis speigkpdgg fmskmaklpk kmfphgakgg vsvegapelg
     3721 dvevkmpeah lgvdikgpek pkgeldvtlp kgkvdidlpe giegkveisa peigkpdggf
     3781 mskmaklpkk mfphgakggv svegspelgd vevkmpeahl gvdikgpekp kgeldvtlpk
     3841 gkvdidlpeg iegkleisap eigkpdggfm skmakfpkkm fphgakggvs vegapelgdv
     3901 qvkmpeahlg vdikgpekpk geldvtlpkg kvdidlpegi egkveisape igkpdggfms
     3961 kmakfpkkmf phgakggvsv egapelgdvq vkmpeahlgv diegpekpkg eldvtlpkgk
     4021 vdidlpegie gkveisapei gkpdggfmsk makfpkkmfp hgtkggvsve gapelgdvev
     4081 kmpeahlgvd ikgpekpkge ldvtlpkgkv didlpegieg kveisapeig kpdggfmskm
     4141 aklpqkmfph gtkggvsveg apelgdiqlk mpeahlgvdi kgpekpkgel damlpkgkvd
     4201 idlpegiegk veisapeigk pdggfmskma klpkkmfshg akgsisvegs pefgdvevkm
     4261 peahlggdik gpekpkgeld valpkgkvdi dipegiegkv eisapeigkp sdggfvskma
     4321 klpkkmfphg tkgsvsiega pevgdvqvkv peaqlkmdik gtgkpefege itwtgkkvav
     4381 elpegiegtv emstpemgrp sdggfmskva klpkkvfshg skggvpfdee tgvagdievk
     4441 mseaalkvdv kypekpesdf salekkidid vpegfkgkve istpelgkps dggflskmsk
     4501 lpkkvlphgs kgeisagevs evsgevpvel pearlkvgvk gperpdsdvd isvsdkkvtl
     4561 dvtkgfegkv evstpdlgkp sdkgfiskvt kfpkkmfphg skggvsvgev sevtgdvhve
     4621 lpdahlkvgv kgperpdsdv dvsmavkkit vdvpegfegk levstpelar spdgslvska
     4681 aklpkklvps genslssvee pslsahvdvp gsdttfkvda klpampvtdv dislsakess
     4741 adipvgvagk veigtpdagk psdkgfmskm akipkkmfhh gpkssgstde hstvaetvdd
     4801 kalgfevqlq kdvkiptkpe advsvsfhek evtvqlpegr egtvelstpg lvgehvpdks
     4861 sqgadvllpg kelptvegqe iiekpggvie vievtahels kplssgllsk vskipkkifp
     4921 rgskskssde dspspgkrel ketpggivev vevtkhdvai hpesthvkqv gtevllepat
     4981 setkpsgaeg lppthitrei kevpggivev vrvtkhivgk lpedkgigep svrttdaslp
     5041 weaelakdps avikrevket peghvevvqv irreasdvpf gsdfpgdles dgtvsrriet
     5101 tvvdgkptvi rrlvtttsdg irqvtehieq gaedagsaas slfakmgefa kglssddpsa
     5161 vttrevkqlp ggavevvsvt krsssspete itsqeqlqss tsgsmhfaes avgkpmeakt
     5221 tiirreisgp etivrtttsv vtsatapgss kpelsadvld kmfgamekpr vddrlrdvlt
     5281 aeggyvakre vtpeeaella qlfpeqstgv avyppaglvp eaaiipeyrc mttaegahhd
     5341 peskekvllk lgralsqddl dapsptqgea amtvaglgea vdtsdqveep aveklelarr
     5401 eskrysqefe qdeplpkamg fktkpseetv vsstqvkgvh dngtvkhtvt tvtkttiiqe
     5461 veepagaprp gesetwasdl dtsqvsvssl pederteptl sssattatad aealsgplar
     5521 tqveekewte tdpdsgevrh iisktthttv tskkpsegap rgdttsttvs hsskdghhgs
     5581 tpsapsecde aesltffqkk effeklseqs ppketssarl gstpsldtal trpavtlkpr
     5641 prsesltsqg eiieesivfa erlrlfqnga qasplpsasi pqvdlvdpap kmeqklqgma
     5701 eigdslqqvp savleglqst eeksdtrllv htsdadkeaf dvrsvqvaed fgdkqfshmr
     5761 vrmpapeetv sktsicssdl pgdkpsplrd aykgslsasr qessqagkpp lpsalggdss
     5821 lclvheattd ltlskagppn qtnkdittfd rqpvreqspt lrssqsqmla sglqmgivag
     5881 sgilqegika spqsailetf sssatemtgl espeirglps eevhagsaee akgidagrig
     5941 avlaaapvai lpvrdiisgk qlemldiaqv sdepnifegc velpsqaesq tcpkektimk
     6001 teyadiqhkl ssafgeslap vesksgvtqg vpkevleald rssqeliask ldedserdtn
     6061 ilsatqgsip hdvfpdhies tldnsaavpm rgaystpsst avcltperle iqatrevaee
     6121 rqekpevlva leayrkstlr rsgedsydrn leefitvlee yeplpagqls dwkraspafe
     6181 psichnseit sklppledkk eletvgsild psenvsellg nhaqsqmlce dtglltdelg
     6241 rtaghyvist aesdrgeclp tlseqapgke reeclnydsa lgvrstsgle sslleeshee
     6301 rsldlarsed eqpslvedsm nvptessvid yqvvghsgvv kdaqtqpkds dfdlheisrt
     6361 gdktdlplsc gddgkksaef sktvsgiaad mvikdtdseh lsqdmlhpta stvlcegrsd
     6421 vpyssdedqh kelsrykeve lvipflrgts ssledevedg gsfssgdlqa ekiaeflttd
     6481 pdtlnkvvhf pqifqdydts ahqepfvpte temnvekhvg fvldemaacs tpcvplksdr
     6541 qteieeasri vesvvhiass lveslhevnh etaikedicd salayaagsp lersevsetl
     6601 sqagppstie kdqrespfgv kemyqvlkes rgsedriqys vtsfgdplfa degdedqerr
     6661 rrgsptaesl ddqaqiekcs hevmmqlldg fsqsdvelae vrtedeapdi eddvpspeag
     6721 sheeksvpss qdnsgdlqpq lsteveigsq asgapaplse ldyelcpdad qlrtsgdeav
     6781 atrvpplepe rdeavaessh apsaaadpaa sagrnaggre rsplleaspp eetaycpeqd
     6841 ispaqeypey ltpdmqvrvg vspteaqpak ekpvrysess vesssevgsp cppsqggtam
     6901 afenvafage dvidatsspi eessspagsr spyevvrdah aeeaaqtalv eaekslrhav
     6961 qeevrarpqs lvteesseqq ehqiqpsifn eafdhspees apekkqlseg llgvglltvs
     7021 dttartpesr rgdsdedeee wadhvmskir grpipptppa spravqsave vqgepweapv
     7081 veqervnves ardmsaerda gvdvmgdrsa tqpqlqefrr tadgkrpqle hipseisded
     7141 lvereqspth itatcrgdrk dsssdrnssp dsdgpkasgs hrrsasgvga ialeavhsqd
     7201 sggnekhshe srdkgaqedd kiteepvdtm fvieeeapad snrsesfcdd aderaartag
     7261 glspslespp qkkdlddgss sgkpdaaspv vlrskskrtd kisdkrssrd ldtghsgset
     7321 dhshyysfel tsdsgktpga srptssefdv nilsgqasse yetcatsqdg evsgsyataa
     7381 asshdtsfat arsslsgssr ssaysvdses sghlgsmevs easetivasa heeldsdies
     7441 ersksvlrep ydgeipesvi rggvehpflp swqpalkdss ptlhgsagar pptaegvfsp
     7501 fqstvpsepg rscvgwpsss lstaesvrsp skpevlngvs aplegqrrlr hnddfmassh
     7561 eeeqlsvses satqsehtwq tsvetmvdav eaqrfscdse rhhrqstmqn svslpfdeav
     7621 vaedshread ddratppfqe vngpveveyv peyddltart lrpvrqqnap dgqteaqgdv
     7681 alleasagda stsreqheee nstelreegf yaetfqgdls seevesaane allldksacs
     7741 fyeychakde yevcntrpav esaslkeafe rpatpepvde iddqedegnl aqgspnlhgk
     7801 dyeafghasa eesvpcsdkt hiaqelsend qykvfsdsgn qsslhdetrs ksvssslera
     7861 mtddvrlyts kcedyilqmh qtykygvglp ktsdggylpe egmdvenvkl lesrlqegng
     7921 gqqdfpydls yqqeyggdgr vehgdlythq eveeegydde rsrtrgrrrl spisdergst
     7981 pdydtlagrk tfgkgsekdd astsslmefe rleaemasgt ksssvgssds lsgrqhqgrs
     8041 gagseqdsvs vnslteferl ekeiveaegv darlrgevaa kldeideghe sqasdpghet
     8101 avydesgatl llcasadvne epdgttddaq lgdieaaqgq evawsrterh flttrtesgh
     8161 svesvdrqss astatqfdad slrereidds esnfgpdtpg wmqkecvdld slhdslhdse
     8221 hhdslqevps kdkdslqlre psadadslqe dhnelqdsly etrdidwdsf radetsgldd
     8281 tsrswhqfps qirttelspt lsrpassplv vpvvveridt ltwvdqdpak acvsdgeslg
     8341 srqqehprnd alvsstdsle psssanthat yqcdtdsmms slnsaeestm vtstdtlehe
     8401 tlclasqgks lslegglfsg gdvteeqntr tpleggvsvd dmeerfrecl lrsaasptne
     8461 eqveerqtmd ekgnlivtrt vkrhvtsepr lhtqtfmgpd aeqrsrefva sfaamepsae
     8521 rdeyegfdaa gntvrvtqhv vvrptiktvt ftgpdarlqm reymrslagg qhssaentpt
     8581 sdsgvgacss lasppplmpd ppaaapssac pglqrlsggd qwmeaesald dcsppvtatr
     8641 vittrttrtm gpdgreqlvt ttteesggee pearlrrsmq gvldsfmaep ptpphldeee
//
LOCUS       XP_077503801            8694 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X10 [Amblyomma
            americanum].
ACCESSION   XP_077503801
VERSION     XP_077503801.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647675.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8694
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..8694
                     /product="uncharacterized protein LOC144114148 isoform
                     X10"
                     /calculated_mol_wt=933447
     Region          24..55
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          38..355
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(57,59,63..64,67..69,71..72,76,79,88,90,92,96..97,
                     100..102,104..105,109,112,121,123,125,129..130,133..135,
                     137..138,142,145)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          57..88
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          90..121
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          123..148
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          156..181
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          189..216
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(218,220,224..225,228..230,232..233,237,240,249,251,
                     253,257..258,261..263,265..266,270,273,282,284,286,
                     290..291,294..296,298..299,303,306)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          218..249
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          251..282
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          284..314
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          298..586
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          317..381
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          383..414
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(416,418,422..423,426..428,430..431,435,438,447,449,
                     451,455..456,459..461,463..464,468,471,480,482,484,
                     488..489,492..494,496..497,501,504)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          416..447
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          449..480
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          464..742
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          482..511
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          515..540
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          548..579
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          581..614
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          615..645
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          647..677
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(680,682,686..687,690..692,694..695,699,702,711,713,
                     715,719..720,723..725,727..728,732,735,744,746,748,
                     752..753,756..758,760..761,765,768)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          680..711
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          713..744
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          718..799
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          746..776
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          957..1059
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1282..1414
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1446..1529
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <1843..2094
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          <4457..4717
                     /region_name="YbbR"
                     /note="Cyclic di-AMP synthase regulator CdaR, YbbR domain
                     [Signal transduction mechanisms]; COG4856"
                     /db_xref="CDD:443884"
     Region          <7122..7435
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..8694
                     /gene="LOC144114148"
                     /coded_by="XM_077647675.1:253..26337"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 maitahdapp gspngqipds aqtdgnasfl raaragnlek vldylkgsid intsnangln
       61 alhlaakegh vnvvsellkr ganvnattkk gnsalhiasl agqeevvkll vqkqanvnvq
      121 sqngftplym aaqenhdgvv rfllanganq slatedgftp lavalqqghd kvvavllend
      181 argkvrlpal hiaskkddck aaalllhseh npdvtsksgf tplhiaahyg nsniasllld
      241 kgadvnfpak hqitplhvaa kwgksnmvkl llekgakida strdgltplh caarsghdqv
      301 veqlleknap itaktkngla plhmasqgdh vdsarillyh kapvddvtvd yltalhvaah
      361 cghvgvakll ldrradpnar alngftplhi ackknrikvv elllkhgasi eattesgltp
      421 lhvasfmgcm niviyliqhg anpdiptvrg etplhlaara nqtdiirill rngahvdaka
      481 relqtalhia srlgnadmvg lllqhgaavd aptkdaytpl hvaaregqde vaallldhga
      541 alaaptkkgf tplhlaakyg nlkvaqlllq kdapvdaqgk ngvtplhvaa hydhvnvall
      601 llekgasphs aarngytplh vaarkdqmdi asslleygak pgaesragft plhlaaqegh
      661 adlaallveh gadcdakakn gltpmhlcaq edrvevatil akhgasldpt tkagytplhv
      721 achfgqtnmi rfllrqganv nattshgytp lhqaaqqght liinlllehr aapnavtnqg
      781 qtalaiaqrl gyisvvetlk vvtetivttt tttvteekyr vvapetmhet fmsdsedeaa
      841 ednmlgdqsf ryltademks lgddslpidv trderitesi hitrepghpa pltqeeerls
      901 ptqahtteai fvgnyapdnv dlsrtpihag sllswdgdsc ssayvlsptk rmwrerflvs
      961 flvdarggam rgcrhsgvrv iipprkaqmp mritcrylrk eklphpppll egeacasril
     1021 evgpagakfl gpvilevphf aslrgkerei silrsdnget wkehtlease eavqevlnes
     1081 fegeelsale dlqtnrivri lttdfpqyfa vvsrtrqevh avgpegglls stvvpqvqai
     1141 fpegaltkki rvglqaqpip aelvtkllgh rvavspivtv eprrrkfhkp itltipvpqa
     1201 atkgminqya gdaptlrllc sitggtnkaq wedvtgstpl tfvndcvsft ttvsarfwlm
     1261 dcrqvseatk fatelyaeam hvpfmakfvv fakrqdpmea qlrvfcmtdd kedktlesqe
     1321 hftevaksrd vevlekksqy lefggnlvpv tksgeqlqlq fqafrenrlp favrvkdphq
     1381 eplgrlafmr qpraaratep pqaplcnlni alpeyeasqn lselvtlekk ygfveetgla
     1441 kpelihradl rlsdiarelg sdwpalaaql dvpeqdvasi rtecpsdlaq qallmlrlwm
     1501 kraggratgn nlekglrmin redivnkcmf nvelvtddve kavakvhldq sgfdtfreel
     1561 gtpkdtslkr dasldvsyde qdlmkeaesa eetssetgsv hekhtgseak gtskaipgvr
     1621 kgspisaaig rivrgsskek aaprmaveet feeepvlkid saevtqqaap eagptapprd
     1681 skkkskkeks psplgpdysl prmrdyhtpe rdftsttdtl ivssikedvd askelatdgk
     1741 vkkekkkekk ekspsppgpd ytlprlrdty tppsdvtaag yaastlpgqg itleqgivke
     1801 plerkskers lspgvvktge ngslgadpsv erqvkqlsqg pedtiyekve rvvketpgki
     1861 vemvhikrqr sskspegrgs pkdknkglps sevvapdisg esrsvpseti gaekkqkkhk
     1921 etsptkqgkk eakevseavd kdaelsasdg klpdsgflsk maklpkkmfp rsgkasasde
     1981 epsslsessp vakkkagklp ksatlpkakp sktplyeiks vdvevdtskl tskgvkgvpa
     2041 kdgegletsr dkepdsrffs kmaripkkmf prssktssse depssasenl psskqrakke
     2101 keisskkkek vvkvkpdskk lstsfevevd rdtlpgagvd inakkpglqe slesesrkkt
     2161 dsmqisfpqs vkavgskgep meysldiegm kslqkepqla vvgefpgkps pdielsltsk
     2221 etapefsdka rghieigtpe hgksskggfm tkmaklprkm fphgfkgdvs veepsveaes
     2281 vhvsvpeaqv klgtkdlkkk gpdmpamelt aslpvgaegs vemsapdlpv sadggliskv
     2341 tqfpkmfshd sqdgvtierp pvvgegidve sslpdvqlkv kgkafekpet kfdvtlperk
     2401 vtpvlpdgae ghievnldem gksyddgfms kmaklpkkmf shgledeiav qepsitqhdi
     2461 hvdipklkde leiratlpek pqvdfdvaip gkqvsldlpe gveskievsa pdvgkptdgg
     2521 fmskvvqlpk kmfphgskgt vpleeapeav gdihakvpea hlkvglkgle kpdfegdiav
     2581 pgkkgavslp egtegkievs tpetgkpsdk gfmskmaklp kkmfphgskg svsveevpev
     2641 drsvkvkvpq gkipeahlkv dikdpekhkd vdialseekv didlpegidk nievtmpevs
     2701 kpseggfisk makfpkkmfp hgakgsvsve eapgidvdvk mpetrismdv kglekpkgev
     2761 dislpkekvd idvpkgfdgk lemsapevgk pseggfmskm akfpkkmfph gakgsvsvee
     2821 apgidvdvkv peahismdvk glekprgevd islpkekvdi dvpkgfdgkl emsapevgkp
     2881 seggfmskma kfpkkmfphg akgslsveet pgidvdvkmp eadlsmdvkg lekpkaeldi
     2941 ampkgkidid mpegvdgkle asapdigkps dggfiskmtk fpkkmfphga kgtvsveape
     3001 lgdvqvnmpe ahlgvdikgp ekpkgeldvt lpkgkvdidl pegiegkvei sapeigkpdg
     3061 gfmskmaklp kkmfphgakg gvsvegspel gdvevkmpea hlgvdikgpe kpkgeldvtl
     3121 pkgkvdidlp egiegkveis apeigkpdgg fmskmaklpk kmfphgakgg vsvegspelg
     3181 dvevkmpeah lgvdikgpek pkgeldvtlp kgkvdidlpe giegkveisa peigkpdggf
     3241 mskmaklpkk mfphgakggv svegspelgd vevkmpeahl gvdikgpekp kgeldvtlpk
     3301 gkvdidlpeg iegkveisap eigkpdggfm skmaklpkkm fshgakggvs vegspelgdv
     3361 qvkmpeahlg vdikgpekpk geldvtlpkg kvdidlpegi egkveisspe igkpdggfms
     3421 kmaklpkkmf phgakggvsv egapelgdve vkmpeahlgv dikgpekpkg eldvtlpkgk
     3481 vdidlpegie gkveisapei gkpdggfmsk maklpkkmfp hgakggvsve gapelgdvev
     3541 kmpeahlgvd ikgpekpkge ldvtlpkgkv didlpegfeg kveisapeig kpdggfmskm
     3601 aklpkkmfph gtkggvsveg apelgdvevk mpeahlgvdi kgpekpkgel dvtlpkgkvd
     3661 idlpegiegk veisspeigk pdggfmskma klpkkmfphg akggvsvega pelgdvevkm
     3721 peahlgvdik gpekpkgeld vtlpkgkvdi dlpegiegkv eisapeigkp dggfmskmak
     3781 lpkkmfphga kggvsvegsp elgdvevkmp eahlgvdikg pekpkgeldv tlpkgkvdid
     3841 lpegiegkle isapeigkpd ggfmskmakf pkkmfphgak ggvsvegape lgdvqvkmpe
     3901 ahlgvdikgp ekpkgeldvt lpkgkvdidl pegiegkvei sapeigkpdg gfmskmakfp
     3961 kkmfphgakg gvsvegapel gdvqvkmpea hlgvdiegpe kpkgeldvtl pkgkvdidlp
     4021 egiegkveis apeigkpdgg fmskmakfpk kmfphgtkgg vsvegapelg dvevkmpeah
     4081 lgvdikgpek pkgeldvtlp kgkvdidlpe giegkveisa peigkpdggf mskmaklpqk
     4141 mfphgtkggv svegapelgd iqlkmpeahl gvdikgpekp kgeldamlpk gkvdidlpeg
     4201 iegkveisap eigkpdggfm skmaklpkkm fshgakgsis vegspefgdv evkmpeahlg
     4261 gdikgpekpk geldvalpkg kvdidipegi egkveisape igkpsdggfv skmaklpkkm
     4321 fphgtkgsvs iegapevgdv qvkvpeaqlk mdikgtgkpe fegeitwtgk kvavelpegi
     4381 egtvemstpe mgrpsdggfm skvaklpkkv fshgskggvp fdeetgvagd ievkmseaal
     4441 kvdvkypekp esdfsalekk ididvpegfk gkveistpel gkpsdggfls kmsklpkkvl
     4501 phgskgeisa gevsevsgev pvelpearlk vgvkgperpd sdvdisvsdk kvtldvtkgf
     4561 egkvevstpd lgkpsdkgfi skvtkfpkkm fphgskggvs vgevsevtgd vhvelpdahl
     4621 kvgvkgperp dsdvdvsmav kkitvdvpeg fegklevstp elarspdgsl vskaaklpkk
     4681 lvpsgensls sveepslsah vdvpgsdttf kvdaklpamp vtdvdislsa kessadipvg
     4741 vagkveigtp dagkpsdkgf mskmakipkk mfhhgpkssg stdehstvae tvddkalgfe
     4801 vqlqkdvkip tkpeadvsvs fhekevtvql pegregtvel stpglvgehv pdkssqgadv
     4861 llpgkelptv egqeiiekpg gvievievta helskplssg llskvskipk kifprgsksk
     4921 ssdedspspg krelketpgg ivevvevtkh dvaihpesth vkqvgtevll epatsetkps
     4981 gaeglppthi treikevpgg ivevvrvtkh ivgklpedkg igepsvrttd aslpweaela
     5041 kdpsavikre vketpeghve vvqvirreas dvpfgsdfpg dlesdgtvsr riettvvdgk
     5101 ptvirrlvtt tsdgirqvte hieqgaedag saasslfakm gefakglssd dpsavttrev
     5161 kqlpggavev vsvtkrssss peteitsqeq lqsstsgsmh faesavgkpm eakttiirre
     5221 isgpetivrt ttsvvtsata pgsskpelsa dvldkmfgam ekprvddrlr dvltaeggyv
     5281 akrevtpeea ellaqlfpeq stgvavyppa glvpeaaiip eyrcmttaeg ahhdpeskek
     5341 vllklgrals qddldapspt qgeaamtvag lgeavdtsdq veepavekle larreskrys
     5401 qefeqdeplp kamgfktkps eetvvsstqv kgvhdngtvk htvttvtktt iiqeveepag
     5461 aprpgesetw asdldtsqvs vsslpedert eptlsssatt atadaealsg plartqveek
     5521 ewtetdpdsg evrhiisktt httvtskkps egaprgdtts ttvshsskdg hhgstpsaps
     5581 ecdeaesltf fqkkeffekl seqsppkets sarlgstpsl dtaltrpavt lkprprsesl
     5641 tsqgeiiees ivfaerlrlf qngaqasplp sasipqvdlv dpapkmeqkl qgmaeigdsl
     5701 qqvpsavleg lqsteeksdt rllvhtsdad keafdvrsvq vaedfgdkqf shmrvrmpap
     5761 eetvsktsic ssdlpgdkps plrdaykgsl sasrqessqa gkpplpsalg gdsslclvhe
     5821 attdltlska gppnqtnkdi ttfdrqpvre qsptlrssqs qmlasglqmg ivagsgilqe
     5881 gikaspqsai letfsssate mtglespeir glpseevhag saeeakgida grigavlaaa
     5941 pvailpvrdi isgkqlemld iaqvsdepni fegcvelpsq aesqtcpkek timkteyadi
     6001 qhklssafge slapvesksg vtqgvpkevl ealdrssqel iaskldedse rdtnilsatq
     6061 gsiphdvfpd hiestldnsa avpmrgayst psstavcltp erleiqatre vaeerqekpe
     6121 vlvaleayrk stlrrsgeds ydrnleefit vleeyeplpa gqlsdwkras pafepsichn
     6181 seitsklppl edkkeletvg sildpsenvs ellgnhaqsq mlcedtgllt delgrtaghy
     6241 vistaesdrg eclptlseqa pgkereecln ydsalgvrst sglessllee sheersldla
     6301 rsedeqpslv edsmnvptes svidyqvvgh sgvvkdaqtq pkdsdfdlhe isrtgdktdl
     6361 plscgddgkk saefsktvsg iaadmvikdt dsehlsqdml hptastvlce grsdvpyssd
     6421 edqhkelsry kevelvipfl rgtssslede vedggsfssg dlqaekiaef lttdpdtlnk
     6481 vvhfpqifqd ydtsahqepf vptetemnve khvgfvldem aacstpcvpl ksdrqteiee
     6541 asrivesvvh iasslveslh evnhetaike dicdsalaya agsplersev setlsqagpp
     6601 stiekdqres pfgvkemyqv lkesrgsedr iqysvtsfgd plfadegded qerrrrgspt
     6661 aeslddqaqi ekcshevmmq lldgfsqsdv elaevrtede apdieddvps peagsheeks
     6721 vpssqdnsgd lqpqlsteve igsqasgapa plseldyelc pdadqlrtsg deavatrvpp
     6781 leperdeava esshapsaaa dpaasagrna ggrersplle asppeetayc peqdispaqe
     6841 ypeyltpdmq vrvgvsptea qpakekpvry sessvessse vgspcppsqg gtamafenva
     6901 fagedvidat sspieesssp agsrspyevv rdahaeeaaq talveaeksl rhavqeevra
     6961 rpqslvtees seqqehqiqp sifneafdhs peesapekkq lsegllgvgl ltvsdttart
     7021 pesrrgdsde deeewadhvm skirgrpipp tppaspravq savevqgepw eapvveqerv
     7081 nvesardmsa erdagvdvmg drsatqpqlq efrrtadgkr pqlehipsei sdedlvereq
     7141 spthitatcr gdrkdsssdr nsspdsdgpk asgshrrsas gvgaialeav hsqdsggnek
     7201 hshesrdkga qeddkiteep vdtmfvieee apadsnrses fcddaderaa rtagglspsl
     7261 esppqkkdld dgsssgkpda aspvvlrsks krtdkisdkr ssrdldtghs gsetdhshyy
     7321 sfeltsdsgk tpgasrptss efdvnilsgq asseyetcat sqdgevsgsy ataaasshdt
     7381 sfatarssls gssrssaysv dsessghlgs mevseaseti vasaheelds diesersksv
     7441 lrepydgeip esvirggveh pflpswqpal kdssptlhgs agarpptaeg vfspfqstvp
     7501 sepgrscvgw pssslstaes vrspskpevl ngvsaplegq rrlrhnddfm assheeeqls
     7561 vsessatqse htwqtsvetm vdaveaqrfs cdserhhrqs tmqnsvslpf deavvaedsh
     7621 readddratp pfqevngpve veyvpeyddl tartlrpvrq qnapdgqtea qgdvalleas
     7681 agdastsreq heeenstelr eegfyaetfq gdlsseeves aaneallldk sacsfyeych
     7741 akdeyevcnt rpavesaslk eaferpatpe pvdeiddqed egnlaqgspn lhgkdyeafg
     7801 hasaeesvpc sdkthiaqel sendqykvfs dsgnqsslhd etrsksvsss leramtddvr
     7861 lytskcedyi lqmhqtykyg vglpktsdgg ylpeegmdve nvkllesrlq egnggqqdfp
     7921 ydlsyqqeyg gdgrvehgdl ythqeveeeg yddersrtrg rrrlspisde rgstpdydtl
     7981 agrktfgkgs ekddastssl meferleaem asgtksssvg ssdslsgrqh qgrsgagseq
     8041 dsvsvnslte ferlekeive aegvdarlrg evaakldeid eghesqasdp ghetavydes
     8101 gatlllcasa dvneepdgtt ddaqlgdiea aqgqevawsr terhflttrt esghsvesvd
     8161 rqssastatq fdadslrere iddsesnfgp dtpgwmqkec vdldslhdsl hdsehhdslq
     8221 evpskdkdsl qlrepsadad slqedhnelq dslyetrdid wdsfradets glddtsrswh
     8281 qfpsqirtte lsptlsrpas splvvpvvve ridtltwvdq dpakacvsdg eslgsrqqeh
     8341 prndalvsst dslepsssan thatyqcdtd smmsslnsae estmvtstdt lehetlclas
     8401 qgkslslegg lfsggdvtee qntrtplegg vsvddmeerf recllrsaas ptneeqveer
     8461 qtmdekgnli vtrtvkrhvt seprlhtqtf mgpdaeqrsr efvasfaame psaerdeyeg
     8521 fdaagntvrv tqhvvvrpti ktvtftgpda rlqmreymrs laggqhssae ntptsdsgvg
     8581 acsslasppp lmpdppaaap ssacpglqrl sggdqwmeae salddcsppv tatrvittrt
     8641 trtmgpdgre qlvttttees ggeepearlr rsmqgvldsf maepptpphl deee
//
LOCUS       XP_077503802            8693 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X11 [Amblyomma
            americanum].
ACCESSION   XP_077503802
VERSION     XP_077503802.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647676.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8693
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..8693
                     /product="uncharacterized protein LOC144114148 isoform
                     X11"
                     /calculated_mol_wt=933317
     Region          24..55
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          38..355
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(57,59,63..64,67..69,71..72,76,79,88,90,92,96..97,
                     100..102,104..105,109,112,121,123,125,129..130,133..135,
                     137..138,142,145)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          57..88
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          90..121
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          123..148
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          156..181
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          189..216
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(218,220,224..225,228..230,232..233,237,240,249,251,
                     253,257..258,261..263,265..266,270,273,282,284,286,
                     290..291,294..296,298..299,303,306)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          218..249
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          251..282
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          284..314
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          298..586
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          317..381
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          383..414
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(416,418,422..423,426..428,430..431,435,438,447,449,
                     451,455..456,459..461,463..464,468,471,480,482,484,
                     488..489,492..494,496..497,501,504)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          416..447
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          449..480
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          464..742
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          482..511
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          515..540
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          548..579
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          581..614
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          615..645
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          647..677
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(680,682,686..687,690..692,694..695,699,702,711,713,
                     715,719..720,723..725,727..728,732,735,744,746,748,
                     752..753,756..758,760..761,765,768)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          680..711
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          713..744
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          718..799
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          746..776
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          957..1059
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1282..1414
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1446..1529
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <1842..2093
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          <4456..4716
                     /region_name="YbbR"
                     /note="Cyclic di-AMP synthase regulator CdaR, YbbR domain
                     [Signal transduction mechanisms]; COG4856"
                     /db_xref="CDD:443884"
     Region          <7121..7434
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..8693
                     /gene="LOC144114148"
                     /coded_by="XM_077647676.1:253..26334"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 maitahdapp gspngqipds aqtdgnasfl raaragnlek vldylkgsid intsnangln
       61 alhlaakegh vnvvsellkr ganvnattkk gnsalhiasl agqeevvkll vqkqanvnvq
      121 sqngftplym aaqenhdgvv rfllanganq slatedgftp lavalqqghd kvvavllend
      181 argkvrlpal hiaskkddck aaalllhseh npdvtsksgf tplhiaahyg nsniasllld
      241 kgadvnfpak hqitplhvaa kwgksnmvkl llekgakida strdgltplh caarsghdqv
      301 veqlleknap itaktkngla plhmasqgdh vdsarillyh kapvddvtvd yltalhvaah
      361 cghvgvakll ldrradpnar alngftplhi ackknrikvv elllkhgasi eattesgltp
      421 lhvasfmgcm niviyliqhg anpdiptvrg etplhlaara nqtdiirill rngahvdaka
      481 relqtalhia srlgnadmvg lllqhgaavd aptkdaytpl hvaaregqde vaallldhga
      541 alaaptkkgf tplhlaakyg nlkvaqlllq kdapvdaqgk ngvtplhvaa hydhvnvall
      601 llekgasphs aarngytplh vaarkdqmdi asslleygak pgaesragft plhlaaqegh
      661 adlaallveh gadcdakakn gltpmhlcaq edrvevatil akhgasldpt tkagytplhv
      721 achfgqtnmi rfllrqganv nattshgytp lhqaaqqght liinlllehr aapnavtnqg
      781 qtalaiaqrl gyisvvetlk vvtetivttt tttvteekyr vvapetmhet fmsdsedeaa
      841 ednmlgdqsf ryltademks lgddslpidv trderitesi hitrepghpa pltqeeerls
      901 ptqahtteai fvgnyapdnv dlsrtpihag sllswdgdsc ssayvlsptk rmwrerflvs
      961 flvdarggam rgcrhsgvrv iipprkaqmp mritcrylrk eklphpppll egeacasril
     1021 evgpagakfl gpvilevphf aslrgkerei silrsdnget wkehtlease eavqevlnes
     1081 fegeelsale dlqtnrivri lttdfpqyfa vvsrtrqevh avgpegglls stvvpqvqai
     1141 fpegaltkki rvglqaqpip aelvtkllgh rvavspivtv eprrrkfhkp itltipvpqa
     1201 atkgminqya gdaptlrllc sitggtnkaq wedvtgstpl tfvndcvsft ttvsarfwlm
     1261 dcrqvseatk fatelyaeam hvpfmakfvv fakrqdpmea qlrvfcmtdd kedktlesqe
     1321 hftevaksrd vevlekksqy lefggnlvpv tksgeqlqlq fqafrenrlp favrvkdphq
     1381 eplgrlafmr qpraaratep pqaplcnlni alpeyeasqn lselvtlekk ygfveetgla
     1441 kpelihradl rlsdiarelg sdwpalaaql dvpeqdvasi rtecpsdlaq qallmlrlwm
     1501 kraggratgn nlekglrmin redivnkcmf nvelvtddve kavakvhldq sgfdtfreel
     1561 gtpkdtslkr dasldvsyde qdlmkaesae etssetgsvh ekhtgseakg tskaipgvrk
     1621 gspisaaigr ivrgsskeka aprmaveetf eeepvlkids aevtqqaape agptapprds
     1681 kkkskkeksp splgpdyslp rmrdyhtper dftsttdtli vssikedvda skelatdgkv
     1741 kkekkkekke kspsppgpdy tlprlrdtyt ppsdvtaagy aastlpgqgi tleqgivkep
     1801 lerkskersl spgvvktgen gslgadpsve rqvkqlsqgp edtiyekver vvketpgkiv
     1861 emvhikrqrs skspegrgsp kdknkglpss evvapdisge srsvpsetig aekkqkkhke
     1921 tsptkqgkke akevseavdk daelsasdgk lpdsgflskm aklpkkmfpr sgkasasdee
     1981 psslsesspv akkkagklpk satlpkakps ktplyeiksv dvevdtsklt skgvkgvpak
     2041 dgegletsrd kepdsrffsk maripkkmfp rssktsssed epssasenlp sskqrakkek
     2101 eisskkkekv vkvkpdskkl stsfevevdr dtlpgagvdi nakkpglqes lesesrkktd
     2161 smqisfpqsv kavgskgepm eysldiegmk slqkepqlav vgefpgkpsp dielsltske
     2221 tapefsdkar ghieigtpeh gksskggfmt kmaklprkmf phgfkgdvsv eepsveaesv
     2281 hvsvpeaqvk lgtkdlkkkg pdmpamelta slpvgaegsv emsapdlpvs adggliskvt
     2341 qfpkmfshds qdgvtierpp vvgegidves slpdvqlkvk gkafekpetk fdvtlperkv
     2401 tpvlpdgaeg hievnldemg ksyddgfmsk maklpkkmfs hgledeiavq epsitqhdih
     2461 vdipklkdel eiratlpekp qvdfdvaipg kqvsldlpeg veskievsap dvgkptdggf
     2521 mskvvqlpkk mfphgskgtv pleeapeavg dihakvpeah lkvglkglek pdfegdiavp
     2581 gkkgavslpe gtegkievst petgkpsdkg fmskmaklpk kmfphgskgs vsveevpevd
     2641 rsvkvkvpqg kipeahlkvd ikdpekhkdv dialseekvd idlpegidkn ievtmpevsk
     2701 pseggfiskm akfpkkmfph gakgsvsvee apgidvdvkm petrismdvk glekpkgevd
     2761 islpkekvdi dvpkgfdgkl emsapevgkp seggfmskma kfpkkmfphg akgsvsveea
     2821 pgidvdvkvp eahismdvkg lekprgevdi slpkekvdid vpkgfdgkle msapevgkps
     2881 eggfmskmak fpkkmfphga kgslsveetp gidvdvkmpe adlsmdvkgl ekpkaeldia
     2941 mpkgkididm pegvdgklea sapdigkpsd ggfiskmtkf pkkmfphgak gtvsveapel
     3001 gdvqvnmpea hlgvdikgpe kpkgeldvtl pkgkvdidlp egiegkveis apeigkpdgg
     3061 fmskmaklpk kmfphgakgg vsvegspelg dvevkmpeah lgvdikgpek pkgeldvtlp
     3121 kgkvdidlpe giegkveisa peigkpdggf mskmaklpkk mfphgakggv svegspelgd
     3181 vevkmpeahl gvdikgpekp kgeldvtlpk gkvdidlpeg iegkveisap eigkpdggfm
     3241 skmaklpkkm fphgakggvs vegspelgdv evkmpeahlg vdikgpekpk geldvtlpkg
     3301 kvdidlpegi egkveisape igkpdggfms kmaklpkkmf shgakggvsv egspelgdvq
     3361 vkmpeahlgv dikgpekpkg eldvtlpkgk vdidlpegie gkveisspei gkpdggfmsk
     3421 maklpkkmfp hgakggvsve gapelgdvev kmpeahlgvd ikgpekpkge ldvtlpkgkv
     3481 didlpegieg kveisapeig kpdggfmskm aklpkkmfph gakggvsveg apelgdvevk
     3541 mpeahlgvdi kgpekpkgel dvtlpkgkvd idlpegfegk veisapeigk pdggfmskma
     3601 klpkkmfphg tkggvsvega pelgdvevkm peahlgvdik gpekpkgeld vtlpkgkvdi
     3661 dlpegiegkv eisspeigkp dggfmskmak lpkkmfphga kggvsvegap elgdvevkmp
     3721 eahlgvdikg pekpkgeldv tlpkgkvdid lpegiegkve isapeigkpd ggfmskmakl
     3781 pkkmfphgak ggvsvegspe lgdvevkmpe ahlgvdikgp ekpkgeldvt lpkgkvdidl
     3841 pegiegklei sapeigkpdg gfmskmakfp kkmfphgakg gvsvegapel gdvqvkmpea
     3901 hlgvdikgpe kpkgeldvtl pkgkvdidlp egiegkveis apeigkpdgg fmskmakfpk
     3961 kmfphgakgg vsvegapelg dvqvkmpeah lgvdiegpek pkgeldvtlp kgkvdidlpe
     4021 giegkveisa peigkpdggf mskmakfpkk mfphgtkggv svegapelgd vevkmpeahl
     4081 gvdikgpekp kgeldvtlpk gkvdidlpeg iegkveisap eigkpdggfm skmaklpqkm
     4141 fphgtkggvs vegapelgdi qlkmpeahlg vdikgpekpk geldamlpkg kvdidlpegi
     4201 egkveisape igkpdggfms kmaklpkkmf shgakgsisv egspefgdve vkmpeahlgg
     4261 dikgpekpkg eldvalpkgk vdidipegie gkveisapei gkpsdggfvs kmaklpkkmf
     4321 phgtkgsvsi egapevgdvq vkvpeaqlkm dikgtgkpef egeitwtgkk vavelpegie
     4381 gtvemstpem grpsdggfms kvaklpkkvf shgskggvpf deetgvagdi evkmseaalk
     4441 vdvkypekpe sdfsalekki didvpegfkg kveistpelg kpsdggflsk msklpkkvlp
     4501 hgskgeisag evsevsgevp velpearlkv gvkgperpds dvdisvsdkk vtldvtkgfe
     4561 gkvevstpdl gkpsdkgfis kvtkfpkkmf phgskggvsv gevsevtgdv hvelpdahlk
     4621 vgvkgperpd sdvdvsmavk kitvdvpegf egklevstpe larspdgslv skaaklpkkl
     4681 vpsgenslss veepslsahv dvpgsdttfk vdaklpampv tdvdislsak essadipvgv
     4741 agkveigtpd agkpsdkgfm skmakipkkm fhhgpkssgs tdehstvaet vddkalgfev
     4801 qlqkdvkipt kpeadvsvsf hekevtvqlp egregtvels tpglvgehvp dkssqgadvl
     4861 lpgkelptve gqeiiekpgg vievievtah elskplssgl lskvskipkk ifprgsksks
     4921 sdedspspgk relketpggi vevvevtkhd vaihpesthv kqvgtevlle patsetkpsg
     4981 aeglppthit reikevpggi vevvrvtkhi vgklpedkgi gepsvrttda slpweaelak
     5041 dpsavikrev ketpeghvev vqvirreasd vpfgsdfpgd lesdgtvsrr iettvvdgkp
     5101 tvirrlvttt sdgirqvteh ieqgaedags aasslfakmg efakglssdd psavttrevk
     5161 qlpggavevv svtkrssssp eteitsqeql qsstsgsmhf aesavgkpme akttiirrei
     5221 sgpetivrtt tsvvtsatap gsskpelsad vldkmfgame kprvddrlrd vltaeggyva
     5281 krevtpeeae llaqlfpeqs tgvavyppag lvpeaaiipe yrcmttaega hhdpeskekv
     5341 llklgralsq ddldapsptq geaamtvagl geavdtsdqv eepaveklel arreskrysq
     5401 efeqdeplpk amgfktkpse etvvsstqvk gvhdngtvkh tvttvtktti iqeveepaga
     5461 prpgesetwa sdldtsqvsv sslpederte ptlsssatta tadaealsgp lartqveeke
     5521 wtetdpdsge vrhiisktth ttvtskkpse gaprgdttst tvshsskdgh hgstpsapse
     5581 cdeaesltff qkkeffekls eqsppketss arlgstpsld taltrpavtl kprprseslt
     5641 sqgeiieesi vfaerlrlfq ngaqasplps asipqvdlvd papkmeqklq gmaeigdslq
     5701 qvpsavlegl qsteeksdtr llvhtsdadk eafdvrsvqv aedfgdkqfs hmrvrmpape
     5761 etvsktsics sdlpgdkpsp lrdaykgsls asrqessqag kpplpsalgg dsslclvhea
     5821 ttdltlskag ppnqtnkdit tfdrqpvreq sptlrssqsq mlasglqmgi vagsgilqeg
     5881 ikaspqsail etfsssatem tglespeirg lpseevhags aeeakgidag rigavlaaap
     5941 vailpvrdii sgkqlemldi aqvsdepnif egcvelpsqa esqtcpkekt imkteyadiq
     6001 hklssafges lapvesksgv tqgvpkevle aldrssqeli askldedser dtnilsatqg
     6061 siphdvfpdh iestldnsaa vpmrgaystp sstavcltpe rleiqatrev aeerqekpev
     6121 lvaleayrks tlrrsgedsy drnleefitv leeyeplpag qlsdwkrasp afepsichns
     6181 eitsklpple dkkeletvgs ildpsenvse llgnhaqsqm lcedtglltd elgrtaghyv
     6241 istaesdrge clptlseqap gkereeclny dsalgvrsts glessllees heersldlar
     6301 sedeqpslve dsmnvptess vidyqvvghs gvvkdaqtqp kdsdfdlhei srtgdktdlp
     6361 lscgddgkks aefsktvsgi aadmvikdtd sehlsqdmlh ptastvlceg rsdvpyssde
     6421 dqhkelsryk evelvipflr gtsssledev edggsfssgd lqaekiaefl ttdpdtlnkv
     6481 vhfpqifqdy dtsahqepfv ptetemnvek hvgfvldema acstpcvplk sdrqteieea
     6541 srivesvvhi asslveslhe vnhetaiked icdsalayaa gsplersevs etlsqagpps
     6601 tiekdqresp fgvkemyqvl kesrgsedri qysvtsfgdp lfadegdedq errrrgspta
     6661 eslddqaqie kcshevmmql ldgfsqsdve laevrtedea pdieddvpsp eagsheeksv
     6721 pssqdnsgdl qpqlstevei gsqasgapap lseldyelcp dadqlrtsgd eavatrvppl
     6781 eperdeavae sshapsaaad paasagrnag grerspllea sppeetaycp eqdispaqey
     6841 peyltpdmqv rvgvspteaq pakekpvrys essvesssev gspcppsqgg tamafenvaf
     6901 agedvidats spieessspa gsrspyevvr dahaeeaaqt alveaekslr havqeevrar
     6961 pqslvteess eqqehqiqps ifneafdhsp eesapekkql segllgvgll tvsdttartp
     7021 esrrgdsded eeewadhvms kirgrpippt ppaspravqs avevqgepwe apvveqervn
     7081 vesardmsae rdagvdvmgd rsatqpqlqe frrtadgkrp qlehipseis dedlvereqs
     7141 pthitatcrg drkdsssdrn sspdsdgpka sgshrrsasg vgaialeavh sqdsggnekh
     7201 shesrdkgaq eddkiteepv dtmfvieeea padsnrsesf cddaderaar tagglspsle
     7261 sppqkkdldd gsssgkpdaa spvvlrsksk rtdkisdkrs srdldtghsg setdhshyys
     7321 feltsdsgkt pgasrptsse fdvnilsgqa sseyetcats qdgevsgsya taaasshdts
     7381 fatarsslsg ssrssaysvd sessghlgsm evseasetiv asaheeldsd iesersksvl
     7441 repydgeipe svirggvehp flpswqpalk dssptlhgsa garpptaegv fspfqstvps
     7501 epgrscvgwp ssslstaesv rspskpevln gvsaplegqr rlrhnddfma ssheeeqlsv
     7561 sessatqseh twqtsvetmv daveaqrfsc dserhhrqst mqnsvslpfd eavvaedshr
     7621 eadddratpp fqevngpvev eyvpeyddlt artlrpvrqq napdgqteaq gdvalleasa
     7681 gdastsreqh eeenstelre egfyaetfqg dlsseevesa aneallldks acsfyeycha
     7741 kdeyevcntr pavesaslke aferpatpep vdeiddqede gnlaqgspnl hgkdyeafgh
     7801 asaeesvpcs dkthiaqels endqykvfsd sgnqsslhde trsksvsssl eramtddvrl
     7861 ytskcedyil qmhqtykygv glpktsdggy lpeegmdven vkllesrlqe gnggqqdfpy
     7921 dlsyqqeygg dgrvehgdly thqeveeegy ddersrtrgr rrlspisder gstpdydtla
     7981 grktfgkgse kddastsslm eferleaema sgtksssvgs sdslsgrqhq grsgagseqd
     8041 svsvnsltef erlekeivea egvdarlrge vaakldeide ghesqasdpg hetavydesg
     8101 atlllcasad vneepdgttd daqlgdieaa qgqevawsrt erhflttrte sghsvesvdr
     8161 qssastatqf dadslrerei ddsesnfgpd tpgwmqkecv dldslhdslh dsehhdslqe
     8221 vpskdkdslq lrepsadads lqedhnelqd slyetrdidw dsfradetsg lddtsrswhq
     8281 fpsqirttel sptlsrpass plvvpvvver idtltwvdqd pakacvsdge slgsrqqehp
     8341 rndalvsstd slepsssant hatyqcdtds mmsslnsaee stmvtstdtl ehetlclasq
     8401 gkslsleggl fsggdvteeq ntrtpleggv svddmeerfr ecllrsaasp tneeqveerq
     8461 tmdekgnliv trtvkrhvts eprlhtqtfm gpdaeqrsre fvasfaamep saerdeyegf
     8521 daagntvrvt qhvvvrptik tvtftgpdar lqmreymrsl aggqhssaen tptsdsgvga
     8581 csslaspppl mpdppaaaps sacpglqrls ggdqwmeaes alddcsppvt atrvittrtt
     8641 rtmgpdgreq lvtttteesg geepearlrr smqgvldsfm aepptpphld eee
//
LOCUS       XP_077503803            8693 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X12 [Amblyomma
            americanum].
ACCESSION   XP_077503803
VERSION     XP_077503803.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647677.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8693
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..8693
                     /product="uncharacterized protein LOC144114148 isoform
                     X12"
                     /calculated_mol_wt=933634
     Region          50..81
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          64..381
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(83,85,89..90,93..95,97..98,102,105,114,116,118,
                     122..123,126..128,130..131,135,138,147,149,151,155..156,
                     159..161,163..164,168,171)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          83..114
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          116..147
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          149..174
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          182..207
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          215..242
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(244,246,250..251,254..256,258..259,263,266,275,277,
                     279,283..284,287..289,291..292,296,299,308,310,312,
                     316..317,320..322,324..325,329,332)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          244..275
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          277..308
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          310..340
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          324..612
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          343..407
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          409..440
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(442,444,448..449,452..454,456..457,461,464,473,475,
                     477,481..482,485..487,489..490,494,497,506,508,510,
                     514..515,518..520,522..523,527,530)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          442..473
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          475..506
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          490..768
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          508..537
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          541..566
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          574..605
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          607..640
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          641..671
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          673..703
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(706,708,712..713,716..718,720..721,725,728,737,739,
                     741,745..746,749..751,753..754,758,761,770,772,774,
                     778..779,782..784,786..787,791,794)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          706..737
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          739..770
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          744..825
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          772..802
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          956..1059
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1282..1414
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1446..1529
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <1842..2093
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          <4456..4716
                     /region_name="YbbR"
                     /note="Cyclic di-AMP synthase regulator CdaR, YbbR domain
                     [Signal transduction mechanisms]; COG4856"
                     /db_xref="CDD:443884"
     Region          <7121..7434
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..8693
                     /gene="LOC144114148"
                     /coded_by="XM_077647677.1:131..26212"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 mtqfvshipv diaassrpfr hhhsdsrepa aaavhreshq qhqqptrktd gnasflraar
       61 agnlekvldy lkgsidints nanglnalhl aakeghvnvv sellkrganv nattkkgnsa
      121 lhiaslagqe evvkllvqkq anvnvqsqng ftplymaaqe nhdgvvrfll anganqslat
      181 edgftplava lqqghdkvva vllendargk vrlpalhias kkddckaaal llhsehnpdv
      241 tsksgftplh iaahygnsni asllldkgad vnfpakhqit plhvaakwgk snmvklllek
      301 gakidastrd gltplhcaar sghdqvveql leknapitak tknglaplhm asqgdhvdsa
      361 rillyhkapv ddvtvdylta lhvaahcghv gvakllldrr adpnaralng ftplhiackk
      421 nrikvvelll khgasieatt esgltplhva sfmgcmnivi yliqhganpd iptvrgetpl
      481 hlaaranqtd iirillrnga hvdakarelq talhiasrlg nadmvglllq hgaavdaptk
      541 daytplhvaa regqdevaal lldhgaalaa ptkkgftplh laakygnlkv aqlllqkdap
      601 vdaqgkngvt plhvaahydh vnvallllek gasphsaarn gytplhvaar kdqmdiassl
      661 leygakpgae sragftplhl aaqeghadla allvehgadc dakakngltp mhlcaqedrv
      721 evatilakhg asldpttkag ytplhvachf gqtnmirfll rqganvnatt shgytplhqa
      781 aqqghtliin lllehraapn avtnqgqtal aiaqrlgyis vvetlkvvte tivttttttv
      841 teekyrvvap etmhetfmsd sedeaaednm lgdqsfrylt ademkslgdd slpidvtrde
      901 ritesihitr epghpapltq eeerlsptqa htteaifvgn yapdnvdlsr tpihagflvs
      961 flvdarggam rgcrhsgvrv iipprkaqmp mritcrylrk eklphpppll egeacasril
     1021 evgpagakfl gpvilevphf aslrgkerei silrsdnget wkehtlease eavqevlnes
     1081 fegeelsale dlqtnrivri lttdfpqyfa vvsrtrqevh avgpegglls stvvpqvqai
     1141 fpegaltkki rvglqaqpip aelvtkllgh rvavspivtv eprrrkfhkp itltipvpqa
     1201 atkgminqya gdaptlrllc sitggtnkaq wedvtgstpl tfvndcvsft ttvsarfwlm
     1261 dcrqvseatk fatelyaeam hvpfmakfvv fakrqdpmea qlrvfcmtdd kedktlesqe
     1321 hftevaksrd vevlekksqy lefggnlvpv tksgeqlqlq fqafrenrlp favrvkdphq
     1381 eplgrlafmr qpraaratep pqaplcnlni alpeyeasqn lselvtlekk ygfveetgla
     1441 kpelihradl rlsdiarelg sdwpalaaql dvpeqdvasi rtecpsdlaq qallmlrlwm
     1501 kraggratgn nlekglrmin redivnkcmf nvelvtddve kavakvhldq sgfdtfreel
     1561 gtpkdtslkr dasldvsyde qdlmkaesae etssetgsvh ekhtgseakg tskaipgvrk
     1621 gspisaaigr ivrgsskeka aprmaveetf eeepvlkids aevtqqaape agptapprds
     1681 kkkskkeksp splgpdyslp rmrdyhtper dftsttdtli vssikedvda skelatdgkv
     1741 kkekkkekke kspsppgpdy tlprlrdtyt ppsdvtaagy aastlpgqgi tleqgivkep
     1801 lerkskersl spgvvktgen gslgadpsve rqvkqlsqgp edtiyekver vvketpgkiv
     1861 emvhikrqrs skspegrgsp kdknkglpss evvapdisge srsvpsetig aekkqkkhke
     1921 tsptkqgkke akevseavdk daelsasdgk lpdsgflskm aklpkkmfpr sgkasasdee
     1981 psslsesspv akkkagklpk satlpkakps ktplyeiksv dvevdtsklt skgvkgvpak
     2041 dgegletsrd kepdsrffsk maripkkmfp rssktsssed epssasenlp sskqrakkek
     2101 eisskkkekv vkvkpdskkl stsfevevdr dtlpgagvdi nakkpglqes lesesrkktd
     2161 smqisfpqsv kavgskgepm eysldiegmk slqkepqlav vgefpgkpsp dielsltske
     2221 tapefsdkar ghieigtpeh gksskggfmt kmaklprkmf phgfkgdvsv eepsveaesv
     2281 hvsvpeaqvk lgtkdlkkkg pdmpamelta slpvgaegsv emsapdlpvs adggliskvt
     2341 qfpkmfshds qdgvtierpp vvgegidves slpdvqlkvk gkafekpetk fdvtlperkv
     2401 tpvlpdgaeg hievnldemg ksyddgfmsk maklpkkmfs hgledeiavq epsitqhdih
     2461 vdipklkdel eiratlpekp qvdfdvaipg kqvsldlpeg veskievsap dvgkptdggf
     2521 mskvvqlpkk mfphgskgtv pleeapeavg dihakvpeah lkvglkglek pdfegdiavp
     2581 gkkgavslpe gtegkievst petgkpsdkg fmskmaklpk kmfphgskgs vsveevpevd
     2641 rsvkvkvpqg kipeahlkvd ikdpekhkdv dialseekvd idlpegidkn ievtmpevsk
     2701 pseggfiskm akfpkkmfph gakgsvsvee apgidvdvkm petrismdvk glekpkgevd
     2761 islpkekvdi dvpkgfdgkl emsapevgkp seggfmskma kfpkkmfphg akgsvsveea
     2821 pgidvdvkvp eahismdvkg lekprgevdi slpkekvdid vpkgfdgkle msapevgkps
     2881 eggfmskmak fpkkmfphga kgslsveetp gidvdvkmpe adlsmdvkgl ekpkaeldia
     2941 mpkgkididm pegvdgklea sapdigkpsd ggfiskmtkf pkkmfphgak gtvsveapel
     3001 gdvqvnmpea hlgvdikgpe kpkgeldvtl pkgkvdidlp egiegkveis apeigkpdgg
     3061 fmskmaklpk kmfphgakgg vsvegspelg dvevkmpeah lgvdikgpek pkgeldvtlp
     3121 kgkvdidlpe giegkveisa peigkpdggf mskmaklpkk mfphgakggv svegspelgd
     3181 vevkmpeahl gvdikgpekp kgeldvtlpk gkvdidlpeg iegkveisap eigkpdggfm
     3241 skmaklpkkm fphgakggvs vegspelgdv evkmpeahlg vdikgpekpk geldvtlpkg
     3301 kvdidlpegi egkveisape igkpdggfms kmaklpkkmf shgakggvsv egspelgdvq
     3361 vkmpeahlgv dikgpekpkg eldvtlpkgk vdidlpegie gkveisspei gkpdggfmsk
     3421 maklpkkmfp hgakggvsve gapelgdvev kmpeahlgvd ikgpekpkge ldvtlpkgkv
     3481 didlpegieg kveisapeig kpdggfmskm aklpkkmfph gakggvsveg apelgdvevk
     3541 mpeahlgvdi kgpekpkgel dvtlpkgkvd idlpegfegk veisapeigk pdggfmskma
     3601 klpkkmfphg tkggvsvega pelgdvevkm peahlgvdik gpekpkgeld vtlpkgkvdi
     3661 dlpegiegkv eisspeigkp dggfmskmak lpkkmfphga kggvsvegap elgdvevkmp
     3721 eahlgvdikg pekpkgeldv tlpkgkvdid lpegiegkve isapeigkpd ggfmskmakl
     3781 pkkmfphgak ggvsvegspe lgdvevkmpe ahlgvdikgp ekpkgeldvt lpkgkvdidl
     3841 pegiegklei sapeigkpdg gfmskmakfp kkmfphgakg gvsvegapel gdvqvkmpea
     3901 hlgvdikgpe kpkgeldvtl pkgkvdidlp egiegkveis apeigkpdgg fmskmakfpk
     3961 kmfphgakgg vsvegapelg dvqvkmpeah lgvdiegpek pkgeldvtlp kgkvdidlpe
     4021 giegkveisa peigkpdggf mskmakfpkk mfphgtkggv svegapelgd vevkmpeahl
     4081 gvdikgpekp kgeldvtlpk gkvdidlpeg iegkveisap eigkpdggfm skmaklpqkm
     4141 fphgtkggvs vegapelgdi qlkmpeahlg vdikgpekpk geldamlpkg kvdidlpegi
     4201 egkveisape igkpdggfms kmaklpkkmf shgakgsisv egspefgdve vkmpeahlgg
     4261 dikgpekpkg eldvalpkgk vdidipegie gkveisapei gkpsdggfvs kmaklpkkmf
     4321 phgtkgsvsi egapevgdvq vkvpeaqlkm dikgtgkpef egeitwtgkk vavelpegie
     4381 gtvemstpem grpsdggfms kvaklpkkvf shgskggvpf deetgvagdi evkmseaalk
     4441 vdvkypekpe sdfsalekki didvpegfkg kveistpelg kpsdggflsk msklpkkvlp
     4501 hgskgeisag evsevsgevp velpearlkv gvkgperpds dvdisvsdkk vtldvtkgfe
     4561 gkvevstpdl gkpsdkgfis kvtkfpkkmf phgskggvsv gevsevtgdv hvelpdahlk
     4621 vgvkgperpd sdvdvsmavk kitvdvpegf egklevstpe larspdgslv skaaklpkkl
     4681 vpsgenslss veepslsahv dvpgsdttfk vdaklpampv tdvdislsak essadipvgv
     4741 agkveigtpd agkpsdkgfm skmakipkkm fhhgpkssgs tdehstvaet vddkalgfev
     4801 qlqkdvkipt kpeadvsvsf hekevtvqlp egregtvels tpglvgehvp dkssqgadvl
     4861 lpgkelptve gqeiiekpgg vievievtah elskplssgl lskvskipkk ifprgsksks
     4921 sdedspspgk relketpggi vevvevtkhd vaihpesthv kqvgtevlle patsetkpsg
     4981 aeglppthit reikevpggi vevvrvtkhi vgklpedkgi gepsvrttda slpweaelak
     5041 dpsavikrev ketpeghvev vqvirreasd vpfgsdfpgd lesdgtvsrr iettvvdgkp
     5101 tvirrlvttt sdgirqvteh ieqgaedags aasslfakmg efakglssdd psavttrevk
     5161 qlpggavevv svtkrssssp eteitsqeql qsstsgsmhf aesavgkpme akttiirrei
     5221 sgpetivrtt tsvvtsatap gsskpelsad vldkmfgame kprvddrlrd vltaeggyva
     5281 krevtpeeae llaqlfpeqs tgvavyppag lvpeaaiipe yrcmttaega hhdpeskekv
     5341 llklgralsq ddldapsptq geaamtvagl geavdtsdqv eepaveklel arreskrysq
     5401 efeqdeplpk amgfktkpse etvvsstqvk gvhdngtvkh tvttvtktti iqeveepaga
     5461 prpgesetwa sdldtsqvsv sslpederte ptlsssatta tadaealsgp lartqveeke
     5521 wtetdpdsge vrhiisktth ttvtskkpse gaprgdttst tvshsskdgh hgstpsapse
     5581 cdeaesltff qkkeffekls eqsppketss arlgstpsld taltrpavtl kprprseslt
     5641 sqgeiieesi vfaerlrlfq ngaqasplps asipqvdlvd papkmeqklq gmaeigdslq
     5701 qvpsavlegl qsteeksdtr llvhtsdadk eafdvrsvqv aedfgdkqfs hmrvrmpape
     5761 etvsktsics sdlpgdkpsp lrdaykgsls asrqessqag kpplpsalgg dsslclvhea
     5821 ttdltlskag ppnqtnkdit tfdrqpvreq sptlrssqsq mlasglqmgi vagsgilqeg
     5881 ikaspqsail etfsssatem tglespeirg lpseevhags aeeakgidag rigavlaaap
     5941 vailpvrdii sgkqlemldi aqvsdepnif egcvelpsqa esqtcpkekt imkteyadiq
     6001 hklssafges lapvesksgv tqgvpkevle aldrssqeli askldedser dtnilsatqg
     6061 siphdvfpdh iestldnsaa vpmrgaystp sstavcltpe rleiqatrev aeerqekpev
     6121 lvaleayrks tlrrsgedsy drnleefitv leeyeplpag qlsdwkrasp afepsichns
     6181 eitsklpple dkkeletvgs ildpsenvse llgnhaqsqm lcedtglltd elgrtaghyv
     6241 istaesdrge clptlseqap gkereeclny dsalgvrsts glessllees heersldlar
     6301 sedeqpslve dsmnvptess vidyqvvghs gvvkdaqtqp kdsdfdlhei srtgdktdlp
     6361 lscgddgkks aefsktvsgi aadmvikdtd sehlsqdmlh ptastvlceg rsdvpyssde
     6421 dqhkelsryk evelvipflr gtsssledev edggsfssgd lqaekiaefl ttdpdtlnkv
     6481 vhfpqifqdy dtsahqepfv ptetemnvek hvgfvldema acstpcvplk sdrqteieea
     6541 srivesvvhi asslveslhe vnhetaiked icdsalayaa gsplersevs etlsqagpps
     6601 tiekdqresp fgvkemyqvl kesrgsedri qysvtsfgdp lfadegdedq errrrgspta
     6661 eslddqaqie kcshevmmql ldgfsqsdve laevrtedea pdieddvpsp eagsheeksv
     6721 pssqdnsgdl qpqlstevei gsqasgapap lseldyelcp dadqlrtsgd eavatrvppl
     6781 eperdeavae sshapsaaad paasagrnag grerspllea sppeetaycp eqdispaqey
     6841 peyltpdmqv rvgvspteaq pakekpvrys essvesssev gspcppsqgg tamafenvaf
     6901 agedvidats spieessspa gsrspyevvr dahaeeaaqt alveaekslr havqeevrar
     6961 pqslvteess eqqehqiqps ifneafdhsp eesapekkql segllgvgll tvsdttartp
     7021 esrrgdsded eeewadhvms kirgrpippt ppaspravqs avevqgepwe apvveqervn
     7081 vesardmsae rdagvdvmgd rsatqpqlqe frrtadgkrp qlehipseis dedlvereqs
     7141 pthitatcrg drkdsssdrn sspdsdgpka sgshrrsasg vgaialeavh sqdsggnekh
     7201 shesrdkgaq eddkiteepv dtmfvieeea padsnrsesf cddaderaar tagglspsle
     7261 sppqkkdldd gsssgkpdaa spvvlrsksk rtdkisdkrs srdldtghsg setdhshyys
     7321 feltsdsgkt pgasrptsse fdvnilsgqa sseyetcats qdgevsgsya taaasshdts
     7381 fatarsslsg ssrssaysvd sessghlgsm evseasetiv asaheeldsd iesersksvl
     7441 repydgeipe svirggvehp flpswqpalk dssptlhgsa garpptaegv fspfqstvps
     7501 epgrscvgwp ssslstaesv rspskpevln gvsaplegqr rlrhnddfma ssheeeqlsv
     7561 sessatqseh twqtsvetmv daveaqrfsc dserhhrqst mqnsvslpfd eavvaedshr
     7621 eadddratpp fqevngpvev eyvpeyddlt artlrpvrqq napdgqteaq gdvalleasa
     7681 gdastsreqh eeenstelre egfyaetfqg dlsseevesa aneallldks acsfyeycha
     7741 kdeyevcntr pavesaslke aferpatpep vdeiddqede gnlaqgspnl hgkdyeafgh
     7801 asaeesvpcs dkthiaqels endqykvfsd sgnqsslhde trsksvsssl eramtddvrl
     7861 ytskcedyil qmhqtykygv glpktsdggy lpeegmdven vkllesrlqe gnggqqdfpy
     7921 dlsyqqeygg dgrvehgdly thqeveeegy ddersrtrgr rrlspisder gstpdydtla
     7981 grktfgkgse kddastsslm eferleaema sgtksssvgs sdslsgrqhq grsgagseqd
     8041 svsvnsltef erlekeivea egvdarlrge vaakldeide ghesqasdpg hetavydesg
     8101 atlllcasad vneepdgttd daqlgdieaa qgqevawsrt erhflttrte sghsvesvdr
     8161 qssastatqf dadslrerei ddsesnfgpd tpgwmqkecv dldslhdslh dsehhdslqe
     8221 vpskdkdslq lrepsadads lqedhnelqd slyetrdidw dsfradetsg lddtsrswhq
     8281 fpsqirttel sptlsrpass plvvpvvver idtltwvdqd pakacvsdge slgsrqqehp
     8341 rndalvsstd slepsssant hatyqcdtds mmsslnsaee stmvtstdtl ehetlclasq
     8401 gkslsleggl fsggdvteeq ntrtpleggv svddmeerfr ecllrsaasp tneeqveerq
     8461 tmdekgnliv trtvkrhvts eprlhtqtfm gpdaeqrsre fvasfaamep saerdeyegf
     8521 daagntvrvt qhvvvrptik tvtftgpdar lqmreymrsl aggqhssaen tptsdsgvga
     8581 csslaspppl mpdppaaaps sacpglqrls ggdqwmeaes alddcsppvt atrvittrtt
     8641 rtmgpdgreq lvtttteesg geepearlrr smqgvldsfm aepptpphld eee
//
LOCUS       XP_077503804            8687 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X13 [Amblyomma
            americanum].
ACCESSION   XP_077503804
VERSION     XP_077503804.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647678.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8687
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..8687
                     /product="uncharacterized protein LOC144114148 isoform
                     X13"
                     /calculated_mol_wt=932995
     Region          10..308
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          10..41
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(43,45,49..50,53..55,57..58,62,65,74,76,78,82..83,
                     86..88,90..91,95,98,107,109,111,115..116,119..121,
                     123..124,128,131)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          43..74
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          76..107
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          109..134
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          142..167
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          175..202
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(204,206,210..211,214..216,218..219,223,226,235,237,
                     239,243..244,247..249,251..252,256,259,268,270,272,
                     276..277,280..282,284..285,289,292)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          204..235
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          237..268
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          270..300
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          284..572
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          303..367
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          369..400
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(402,404,408..409,412..414,416..417,421,424,433,435,
                     437,441..442,445..447,449..450,454,457,466,468,470,
                     474..475,478..480,482..483,487,490)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          402..433
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          435..466
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          450..728
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          468..497
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          501..526
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          534..565
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          567..600
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          601..631
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          633..663
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(666,668,672..673,676..678,680..681,685,688,697,699,
                     701,705..706,709..711,713..714,718,721,730,732,734,
                     738..739,742..744,746..747,751,754)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          666..697
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          699..730
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          704..785
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          732..762
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          950..1052
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1275..1407
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1439..1522
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <1836..2087
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          <4450..4710
                     /region_name="YbbR"
                     /note="Cyclic di-AMP synthase regulator CdaR, YbbR domain
                     [Signal transduction mechanisms]; COG4856"
                     /db_xref="CDD:443884"
     Region          <7115..7428
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..8687
                     /gene="LOC144114148"
                     /coded_by="XM_077647678.1:273..26336"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 mvtegeqqtd gnasflraar agnlekvldy lkgsidints nanglnalhl aakeghvnvv
       61 sellkrganv nattkkgnsa lhiaslagqe evvkllvqkq anvnvqsqng ftplymaaqe
      121 nhdgvvrfll anganqslat edgftplava lqqghdkvva vllendargk vrlpalhias
      181 kkddckaaal llhsehnpdv tsksgftplh iaahygnsni asllldkgad vnfpakhqit
      241 plhvaakwgk snmvklllek gakidastrd gltplhcaar sghdqvveql leknapitak
      301 tknglaplhm asqgdhvdsa rillyhkapv ddvtvdylta lhvaahcghv gvakllldrr
      361 adpnaralng ftplhiackk nrikvvelll khgasieatt esgltplhva sfmgcmnivi
      421 yliqhganpd iptvrgetpl hlaaranqtd iirillrnga hvdakarelq talhiasrlg
      481 nadmvglllq hgaavdaptk daytplhvaa regqdevaal lldhgaalaa ptkkgftplh
      541 laakygnlkv aqlllqkdap vdaqgkngvt plhvaahydh vnvallllek gasphsaarn
      601 gytplhvaar kdqmdiassl leygakpgae sragftplhl aaqeghadla allvehgadc
      661 dakakngltp mhlcaqedrv evatilakhg asldpttkag ytplhvachf gqtnmirfll
      721 rqganvnatt shgytplhqa aqqghtliin lllehraapn avtnqgqtal aiaqrlgyis
      781 vvetlkvvte tivttttttv teekyrvvap etmhetfmsd sedeaaednm lgdqsfrylt
      841 ademkslgdd slpidvtrde ritesihitr epghpapltq eeerlsptqa htteaifvgn
      901 yapdnvdlsr tpihagslls wdgdscssay vlsptkrmwr essklkwktf lvsflvdarg
      961 gamrgcrhsg vrviipprka qmpmritcry lrkeklphpp pllegeacas rilevgpaga
     1021 kflgpvilev phfaslrgke reisilrsdn getwkehtle aseeavqevl nesfegeels
     1081 aledlqtnri vrilttdfpq yfavvsrtrq evhavgpegg llsstvvpqv qaifpegalt
     1141 kkirvglqaq pipaelvtkl lghrvavspi vtveprrrkf hkpitltipv pqaatkgmin
     1201 qyagdaptlr llcsitggtn kaqwedvtgs tpltfvndcv sftttvsarf wlmdcrqvse
     1261 atkfatelya eamhvpfmak fvvfakrqdp meaqlrvfcm tddkedktle sqehftevak
     1321 srdvevlekk sqylefggnl vpvtksgeql qlqfqafren rlpfavrvkd phqeplgrla
     1381 fmrqpraara teppqaplcn lnialpeyea sqnlselvtl ekkygfveet glakpelihr
     1441 adlrlsdiar elgsdwpala aqldvpeqdv asirtecpsd laqqallmlr lwmkraggra
     1501 tgnnlekglr minredivnk cmfnvelvtd dvekavakvh ldqsgfdtfr eelgtpkdts
     1561 lkrdasldvs ydeqdlmkea esaeetsset gsvhekhtgs eakgtskaip gvrkgspisa
     1621 aigrivrgss kekaaprmav eetfeeepvl kidsaevtqq aapeagptap prdskkkskk
     1681 ekspsplgpd yslprmrdyh tperdftstt dtlivssike dvdaskelat dgkvkkekkk
     1741 ekkekspspp gpdytlprlr dtytppsdvt aagyaastlp gqgitleqgi vkeplerksk
     1801 erslspgvvk tgengslgad psverqvkql sqgpedtiye kvervvketp gkivemvhik
     1861 rqrsskspeg rgspkdknkg lpssevvapd isgesrsvps etigaekkqk khketsptkq
     1921 gkkeakevse avdkdaelsa sdgklpdsgf lskmaklpkk mfprsgkasa sdeepsslse
     1981 sspvakkkag klpksatlpk akpsktplye iksvdvevdt skltskgvkg vpakdgegle
     2041 tsrdkepdsr ffskmaripk kmfprsskts ssedepssas enlpsskqra kkekeisskk
     2101 kekvvkvkpd skklstsfev evdrdtlpga gvdinakkpg lqeslesesr kktdsmqisf
     2161 pqsvkavgsk gepmeysldi egmkslqkep qlavvgefpg kpspdielsl tsketapefs
     2221 dkarghieig tpehgksskg gfmtkmaklp rkmfphgfkg dvsveepsve aesvhvsvpe
     2281 aqvklgtkdl kkkgpdmpam eltaslpvga egsvemsapd lpvsadggli skvtqfpkmf
     2341 shdsqdgvti erppvvgegi dvesslpdvq lkvkgkafek petkfdvtlp erkvtpvlpd
     2401 gaeghievnl demgksyddg fmskmaklpk kmfshglede iavqepsitq hdihvdipkl
     2461 kdeleiratl pekpqvdfdv aipgkqvsld lpegveskie vsapdvgkpt dggfmskvvq
     2521 lpkkmfphgs kgtvpleeap eavgdihakv peahlkvglk glekpdfegd iavpgkkgav
     2581 slpegtegki evstpetgkp sdkgfmskma klpkkmfphg skgsvsveev pevdrsvkvk
     2641 vpqgkipeah lkvdikdpek hkdvdialse ekvdidlpeg idknievtmp evskpseggf
     2701 iskmakfpkk mfphgakgsv sveeapgidv dvkmpetris mdvkglekpk gevdislpke
     2761 kvdidvpkgf dgklemsape vgkpseggfm skmakfpkkm fphgakgsvs veeapgidvd
     2821 vkvpeahism dvkglekprg evdislpkek vdidvpkgfd gklemsapev gkpseggfms
     2881 kmakfpkkmf phgakgslsv eetpgidvdv kmpeadlsmd vkglekpkae ldiampkgki
     2941 didmpegvdg kleasapdig kpsdggfisk mtkfpkkmfp hgakgtvsve apelgdvqvn
     3001 mpeahlgvdi kgpekpkgel dvtlpkgkvd idlpegiegk veisapeigk pdggfmskma
     3061 klpkkmfphg akggvsvegs pelgdvevkm peahlgvdik gpekpkgeld vtlpkgkvdi
     3121 dlpegiegkv eisapeigkp dggfmskmak lpkkmfphga kggvsvegsp elgdvevkmp
     3181 eahlgvdikg pekpkgeldv tlpkgkvdid lpegiegkve isapeigkpd ggfmskmakl
     3241 pkkmfphgak ggvsvegspe lgdvevkmpe ahlgvdikgp ekpkgeldvt lpkgkvdidl
     3301 pegiegkvei sapeigkpdg gfmskmaklp kkmfshgakg gvsvegspel gdvqvkmpea
     3361 hlgvdikgpe kpkgeldvtl pkgkvdidlp egiegkveis speigkpdgg fmskmaklpk
     3421 kmfphgakgg vsvegapelg dvevkmpeah lgvdikgpek pkgeldvtlp kgkvdidlpe
     3481 giegkveisa peigkpdggf mskmaklpkk mfphgakggv svegapelgd vevkmpeahl
     3541 gvdikgpekp kgeldvtlpk gkvdidlpeg fegkveisap eigkpdggfm skmaklpkkm
     3601 fphgtkggvs vegapelgdv evkmpeahlg vdikgpekpk geldvtlpkg kvdidlpegi
     3661 egkveisspe igkpdggfms kmaklpkkmf phgakggvsv egapelgdve vkmpeahlgv
     3721 dikgpekpkg eldvtlpkgk vdidlpegie gkveisapei gkpdggfmsk maklpkkmfp
     3781 hgakggvsve gspelgdvev kmpeahlgvd ikgpekpkge ldvtlpkgkv didlpegieg
     3841 kleisapeig kpdggfmskm akfpkkmfph gakggvsveg apelgdvqvk mpeahlgvdi
     3901 kgpekpkgel dvtlpkgkvd idlpegiegk veisapeigk pdggfmskma kfpkkmfphg
     3961 akggvsvega pelgdvqvkm peahlgvdie gpekpkgeld vtlpkgkvdi dlpegiegkv
     4021 eisapeigkp dggfmskmak fpkkmfphgt kggvsvegap elgdvevkmp eahlgvdikg
     4081 pekpkgeldv tlpkgkvdid lpegiegkve isapeigkpd ggfmskmakl pqkmfphgtk
     4141 ggvsvegape lgdiqlkmpe ahlgvdikgp ekpkgeldam lpkgkvdidl pegiegkvei
     4201 sapeigkpdg gfmskmaklp kkmfshgakg sisvegspef gdvevkmpea hlggdikgpe
     4261 kpkgeldval pkgkvdidip egiegkveis apeigkpsdg gfvskmaklp kkmfphgtkg
     4321 svsiegapev gdvqvkvpea qlkmdikgtg kpefegeitw tgkkvavelp egiegtvems
     4381 tpemgrpsdg gfmskvaklp kkvfshgskg gvpfdeetgv agdievkmse aalkvdvkyp
     4441 ekpesdfsal ekkididvpe gfkgkveist pelgkpsdgg flskmsklpk kvlphgskge
     4501 isagevsevs gevpvelpea rlkvgvkgpe rpdsdvdisv sdkkvtldvt kgfegkvevs
     4561 tpdlgkpsdk gfiskvtkfp kkmfphgskg gvsvgevsev tgdvhvelpd ahlkvgvkgp
     4621 erpdsdvdvs mavkkitvdv pegfegklev stpelarspd gslvskaakl pkklvpsgen
     4681 slssveepsl sahvdvpgsd ttfkvdaklp ampvtdvdis lsakessadi pvgvagkvei
     4741 gtpdagkpsd kgfmskmaki pkkmfhhgpk ssgstdehst vaetvddkal gfevqlqkdv
     4801 kiptkpeadv svsfhekevt vqlpegregt velstpglvg ehvpdkssqg advllpgkel
     4861 ptvegqeiie kpggvievie vtahelskpl ssgllskvsk ipkkifprgs kskssdedsp
     4921 spgkrelket pggivevvev tkhdvaihpe sthvkqvgte vllepatset kpsgaeglpp
     4981 thitreikev pggivevvrv tkhivgklpe dkgigepsvr ttdaslpwea elakdpsavi
     5041 krevketpeg hvevvqvirr easdvpfgsd fpgdlesdgt vsrriettvv dgkptvirrl
     5101 vtttsdgirq vtehieqgae dagsaasslf akmgefakgl ssddpsavtt revkqlpgga
     5161 vevvsvtkrs ssspeteits qeqlqsstsg smhfaesavg kpmeakttii rreisgpeti
     5221 vrtttsvvts atapgsskpe lsadvldkmf gamekprvdd rlrdvltaeg gyvakrevtp
     5281 eeaellaqlf peqstgvavy ppaglvpeaa iipeyrcmtt aegahhdpes kekvllklgr
     5341 alsqddldap sptqgeaamt vaglgeavdt sdqveepave klelarresk rysqefeqde
     5401 plpkamgfkt kpseetvvss tqvkgvhdng tvkhtvttvt kttiiqevee pagaprpges
     5461 etwasdldts qvsvsslped erteptlsss attatadaea lsgplartqv eekewtetdp
     5521 dsgevrhiis ktthttvtsk kpsegaprgd ttsttvshss kdghhgstps apsecdeaes
     5581 ltffqkkeff eklseqsppk etssarlgst psldtaltrp avtlkprprs esltsqgeii
     5641 eesivfaerl rlfqngaqas plpsasipqv dlvdpapkme qklqgmaeig dslqqvpsav
     5701 leglqsteek sdtrllvhts dadkeafdvr svqvaedfgd kqfshmrvrm papeetvskt
     5761 sicssdlpgd kpsplrdayk gslsasrqes sqagkpplps alggdsslcl vheattdltl
     5821 skagppnqtn kdittfdrqp vreqsptlrs sqsqmlasgl qmgivagsgi lqegikaspq
     5881 sailetfsss atemtglesp eirglpseev hagsaeeakg idagrigavl aaapvailpv
     5941 rdiisgkqle mldiaqvsde pnifegcvel psqaesqtcp kektimktey adiqhklssa
     6001 fgeslapves ksgvtqgvpk evlealdrss qeliasklde dserdtnils atqgsiphdv
     6061 fpdhiestld nsaavpmrga ystpsstavc ltperleiqa trevaeerqe kpevlvalea
     6121 yrkstlrrsg edsydrnlee fitvleeyep lpagqlsdwk raspafepsi chnseitskl
     6181 ppledkkele tvgsildpse nvsellgnha qsqmlcedtg lltdelgrta ghyvistaes
     6241 drgeclptls eqapgkeree clnydsalgv rstsglessl leesheersl dlarsedeqp
     6301 slvedsmnvp tessvidyqv vghsgvvkda qtqpkdsdfd lheisrtgdk tdlplscgdd
     6361 gkksaefskt vsgiaadmvi kdtdsehlsq dmlhptastv lcegrsdvpy ssdedqhkel
     6421 srykevelvi pflrgtsssl edevedggsf ssgdlqaeki aeflttdpdt lnkvvhfpqi
     6481 fqdydtsahq epfvptetem nvekhvgfvl demaacstpc vplksdrqte ieeasrives
     6541 vvhiasslve slhevnheta ikedicdsal ayaagspler sevsetlsqa gppstiekdq
     6601 respfgvkem yqvlkesrgs edriqysvts fgdplfadeg dedqerrrrg sptaeslddq
     6661 aqiekcshev mmqlldgfsq sdvelaevrt edeapdiedd vpspeagshe eksvpssqdn
     6721 sgdlqpqlst eveigsqasg apaplseldy elcpdadqlr tsgdeavatr vppleperde
     6781 avaesshaps aaadpaasag rnaggrersp lleasppeet aycpeqdisp aqeypeyltp
     6841 dmqvrvgvsp teaqpakekp vrysessves ssevgspcpp sqggtamafe nvafagedvi
     6901 datsspiees sspagsrspy evvrdahaee aaqtalveae kslrhavqee vrarpqslvt
     6961 eesseqqehq iqpsifneaf dhspeesape kkqlsegllg vglltvsdtt artpesrrgd
     7021 sdedeeewad hvmskirgrp ipptppaspr avqsavevqg epweapvveq ervnvesard
     7081 msaerdagvd vmgdrsatqp qlqefrrtad gkrpqlehip seisdedlve reqspthita
     7141 tcrgdrkdss sdrnsspdsd gpkasgshrr sasgvgaial eavhsqdsgg nekhshesrd
     7201 kgaqeddkit eepvdtmfvi eeeapadsnr sesfcddade raartaggls pslesppqkk
     7261 dlddgsssgk pdaaspvvlr skskrtdkis dkrssrdldt ghsgsetdhs hyysfeltsd
     7321 sgktpgasrp tssefdvnil sgqasseyet catsqdgevs gsyataaass hdtsfatars
     7381 slsgssrssa ysvdsessgh lgsmevseas etivasahee ldsdiesers ksvlrepydg
     7441 eipesvirgg vehpflpswq palkdssptl hgsagarppt aegvfspfqs tvpsepgrsc
     7501 vgwpssslst aesvrspskp evlngvsapl egqrrlrhnd dfmassheee qlsvsessat
     7561 qsehtwqtsv etmvdaveaq rfscdserhh rqstmqnsvs lpfdeavvae dshreadddr
     7621 atppfqevng pveveyvpey ddltartlrp vrqqnapdgq teaqgdvall easagdasts
     7681 reqheeenst elreegfyae tfqgdlssee vesaaneall ldksacsfye ychakdeyev
     7741 cntrpavesa slkeaferpa tpepvdeidd qedegnlaqg spnlhgkdye afghasaees
     7801 vpcsdkthia qelsendqyk vfsdsgnqss lhdetrsksv sssleramtd dvrlytskce
     7861 dyilqmhqty kygvglpkts dggylpeegm dvenvklles rlqegnggqq dfpydlsyqq
     7921 eyggdgrveh gdlythqeve eegyddersr trgrrrlspi sdergstpdy dtlagrktfg
     7981 kgsekddast sslmeferle aemasgtkss svgssdslsg rqhqgrsgag seqdsvsvns
     8041 lteferleke iveaegvdar lrgevaakld eideghesqa sdpghetavy desgatlllc
     8101 asadvneepd gttddaqlgd ieaaqgqeva wsrterhflt trtesghsve svdrqssast
     8161 atqfdadslr ereiddsesn fgpdtpgwmq kecvdldslh dslhdsehhd slqevpskdk
     8221 dslqlrepsa dadslqedhn elqdslyetr didwdsfrad etsglddtsr swhqfpsqir
     8281 ttelsptlsr passplvvpv vveridtltw vdqdpakacv sdgeslgsrq qehprndalv
     8341 sstdslepss santhatyqc dtdsmmssln saeestmvts tdtlehetlc lasqgkslsl
     8401 egglfsggdv teeqntrtpl eggvsvddme erfrecllrs aasptneeqv eerqtmdekg
     8461 nlivtrtvkr hvtseprlht qtfmgpdaeq rsrefvasfa amepsaerde yegfdaagnt
     8521 vrvtqhvvvr ptiktvtftg pdarlqmrey mrslaggqhs saentptsds gvgacsslas
     8581 ppplmpdppa aapssacpgl qrlsggdqwm eaesalddcs ppvtatrvit trttrtmgpd
     8641 greqlvtttt eesggeepea rlrrsmqgvl dsfmaepptp phldeee
//
LOCUS       XP_077503805            8686 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X14 [Amblyomma
            americanum].
ACCESSION   XP_077503805
VERSION     XP_077503805.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647679.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8686
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..8686
                     /product="uncharacterized protein LOC144114148 isoform
                     X14"
                     /calculated_mol_wt=932866
     Region          10..308
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          10..41
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(43,45,49..50,53..55,57..58,62,65,74,76,78,82..83,
                     86..88,90..91,95,98,107,109,111,115..116,119..121,
                     123..124,128,131)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          43..74
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          76..107
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          109..134
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          142..167
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          175..202
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(204,206,210..211,214..216,218..219,223,226,235,237,
                     239,243..244,247..249,251..252,256,259,268,270,272,
                     276..277,280..282,284..285,289,292)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          204..235
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          237..268
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          270..300
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          284..572
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          303..367
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          369..400
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(402,404,408..409,412..414,416..417,421,424,433,435,
                     437,441..442,445..447,449..450,454,457,466,468,470,
                     474..475,478..480,482..483,487,490)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          402..433
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          435..466
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          450..728
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          468..497
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          501..526
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          534..565
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          567..600
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          601..631
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          633..663
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(666,668,672..673,676..678,680..681,685,688,697,699,
                     701,705..706,709..711,713..714,718,721,730,732,734,
                     738..739,742..744,746..747,751,754)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          666..697
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          699..730
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          704..785
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          732..762
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          950..1052
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1275..1407
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1439..1522
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <1835..2086
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          <4449..4709
                     /region_name="YbbR"
                     /note="Cyclic di-AMP synthase regulator CdaR, YbbR domain
                     [Signal transduction mechanisms]; COG4856"
                     /db_xref="CDD:443884"
     Region          <7114..7427
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..8686
                     /gene="LOC144114148"
                     /coded_by="XM_077647679.1:273..26333"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 mvtegeqqtd gnasflraar agnlekvldy lkgsidints nanglnalhl aakeghvnvv
       61 sellkrganv nattkkgnsa lhiaslagqe evvkllvqkq anvnvqsqng ftplymaaqe
      121 nhdgvvrfll anganqslat edgftplava lqqghdkvva vllendargk vrlpalhias
      181 kkddckaaal llhsehnpdv tsksgftplh iaahygnsni asllldkgad vnfpakhqit
      241 plhvaakwgk snmvklllek gakidastrd gltplhcaar sghdqvveql leknapitak
      301 tknglaplhm asqgdhvdsa rillyhkapv ddvtvdylta lhvaahcghv gvakllldrr
      361 adpnaralng ftplhiackk nrikvvelll khgasieatt esgltplhva sfmgcmnivi
      421 yliqhganpd iptvrgetpl hlaaranqtd iirillrnga hvdakarelq talhiasrlg
      481 nadmvglllq hgaavdaptk daytplhvaa regqdevaal lldhgaalaa ptkkgftplh
      541 laakygnlkv aqlllqkdap vdaqgkngvt plhvaahydh vnvallllek gasphsaarn
      601 gytplhvaar kdqmdiassl leygakpgae sragftplhl aaqeghadla allvehgadc
      661 dakakngltp mhlcaqedrv evatilakhg asldpttkag ytplhvachf gqtnmirfll
      721 rqganvnatt shgytplhqa aqqghtliin lllehraapn avtnqgqtal aiaqrlgyis
      781 vvetlkvvte tivttttttv teekyrvvap etmhetfmsd sedeaaednm lgdqsfrylt
      841 ademkslgdd slpidvtrde ritesihitr epghpapltq eeerlsptqa htteaifvgn
      901 yapdnvdlsr tpihagslls wdgdscssay vlsptkrmwr essklkwktf lvsflvdarg
      961 gamrgcrhsg vrviipprka qmpmritcry lrkeklphpp pllegeacas rilevgpaga
     1021 kflgpvilev phfaslrgke reisilrsdn getwkehtle aseeavqevl nesfegeels
     1081 aledlqtnri vrilttdfpq yfavvsrtrq evhavgpegg llsstvvpqv qaifpegalt
     1141 kkirvglqaq pipaelvtkl lghrvavspi vtveprrrkf hkpitltipv pqaatkgmin
     1201 qyagdaptlr llcsitggtn kaqwedvtgs tpltfvndcv sftttvsarf wlmdcrqvse
     1261 atkfatelya eamhvpfmak fvvfakrqdp meaqlrvfcm tddkedktle sqehftevak
     1321 srdvevlekk sqylefggnl vpvtksgeql qlqfqafren rlpfavrvkd phqeplgrla
     1381 fmrqpraara teppqaplcn lnialpeyea sqnlselvtl ekkygfveet glakpelihr
     1441 adlrlsdiar elgsdwpala aqldvpeqdv asirtecpsd laqqallmlr lwmkraggra
     1501 tgnnlekglr minredivnk cmfnvelvtd dvekavakvh ldqsgfdtfr eelgtpkdts
     1561 lkrdasldvs ydeqdlmkae saeetssetg svhekhtgse akgtskaipg vrkgspisaa
     1621 igrivrgssk ekaaprmave etfeeepvlk idsaevtqqa apeagptapp rdskkkskke
     1681 kspsplgpdy slprmrdyht perdftsttd tlivssiked vdaskelatd gkvkkekkke
     1741 kkekspsppg pdytlprlrd tytppsdvta agyaastlpg qgitleqgiv keplerkske
     1801 rslspgvvkt gengslgadp sverqvkqls qgpedtiyek vervvketpg kivemvhikr
     1861 qrsskspegr gspkdknkgl pssevvapdi sgesrsvpse tigaekkqkk hketsptkqg
     1921 kkeakevsea vdkdaelsas dgklpdsgfl skmaklpkkm fprsgkasas deepsslses
     1981 spvakkkagk lpksatlpka kpsktplyei ksvdvevdts kltskgvkgv pakdgeglet
     2041 srdkepdsrf fskmaripkk mfprssktss sedepssase nlpsskqrak kekeisskkk
     2101 ekvvkvkpds kklstsfeve vdrdtlpgag vdinakkpgl qeslesesrk ktdsmqisfp
     2161 qsvkavgskg epmeysldie gmkslqkepq lavvgefpgk pspdielslt sketapefsd
     2221 karghieigt pehgksskgg fmtkmaklpr kmfphgfkgd vsveepsvea esvhvsvpea
     2281 qvklgtkdlk kkgpdmpame ltaslpvgae gsvemsapdl pvsadgglis kvtqfpkmfs
     2341 hdsqdgvtie rppvvgegid vesslpdvql kvkgkafekp etkfdvtlpe rkvtpvlpdg
     2401 aeghievnld emgksyddgf mskmaklpkk mfshgledei avqepsitqh dihvdipklk
     2461 deleiratlp ekpqvdfdva ipgkqvsldl pegveskiev sapdvgkptd ggfmskvvql
     2521 pkkmfphgsk gtvpleeape avgdihakvp eahlkvglkg lekpdfegdi avpgkkgavs
     2581 lpegtegkie vstpetgkps dkgfmskmak lpkkmfphgs kgsvsveevp evdrsvkvkv
     2641 pqgkipeahl kvdikdpekh kdvdialsee kvdidlpegi dknievtmpe vskpseggfi
     2701 skmakfpkkm fphgakgsvs veeapgidvd vkmpetrism dvkglekpkg evdislpkek
     2761 vdidvpkgfd gklemsapev gkpseggfms kmakfpkkmf phgakgsvsv eeapgidvdv
     2821 kvpeahismd vkglekprge vdislpkekv didvpkgfdg klemsapevg kpseggfmsk
     2881 makfpkkmfp hgakgslsve etpgidvdvk mpeadlsmdv kglekpkael diampkgkid
     2941 idmpegvdgk leasapdigk psdggfiskm tkfpkkmfph gakgtvsvea pelgdvqvnm
     3001 peahlgvdik gpekpkgeld vtlpkgkvdi dlpegiegkv eisapeigkp dggfmskmak
     3061 lpkkmfphga kggvsvegsp elgdvevkmp eahlgvdikg pekpkgeldv tlpkgkvdid
     3121 lpegiegkve isapeigkpd ggfmskmakl pkkmfphgak ggvsvegspe lgdvevkmpe
     3181 ahlgvdikgp ekpkgeldvt lpkgkvdidl pegiegkvei sapeigkpdg gfmskmaklp
     3241 kkmfphgakg gvsvegspel gdvevkmpea hlgvdikgpe kpkgeldvtl pkgkvdidlp
     3301 egiegkveis apeigkpdgg fmskmaklpk kmfshgakgg vsvegspelg dvqvkmpeah
     3361 lgvdikgpek pkgeldvtlp kgkvdidlpe giegkveiss peigkpdggf mskmaklpkk
     3421 mfphgakggv svegapelgd vevkmpeahl gvdikgpekp kgeldvtlpk gkvdidlpeg
     3481 iegkveisap eigkpdggfm skmaklpkkm fphgakggvs vegapelgdv evkmpeahlg
     3541 vdikgpekpk geldvtlpkg kvdidlpegf egkveisape igkpdggfms kmaklpkkmf
     3601 phgtkggvsv egapelgdve vkmpeahlgv dikgpekpkg eldvtlpkgk vdidlpegie
     3661 gkveisspei gkpdggfmsk maklpkkmfp hgakggvsve gapelgdvev kmpeahlgvd
     3721 ikgpekpkge ldvtlpkgkv didlpegieg kveisapeig kpdggfmskm aklpkkmfph
     3781 gakggvsveg spelgdvevk mpeahlgvdi kgpekpkgel dvtlpkgkvd idlpegiegk
     3841 leisapeigk pdggfmskma kfpkkmfphg akggvsvega pelgdvqvkm peahlgvdik
     3901 gpekpkgeld vtlpkgkvdi dlpegiegkv eisapeigkp dggfmskmak fpkkmfphga
     3961 kggvsvegap elgdvqvkmp eahlgvdieg pekpkgeldv tlpkgkvdid lpegiegkve
     4021 isapeigkpd ggfmskmakf pkkmfphgtk ggvsvegape lgdvevkmpe ahlgvdikgp
     4081 ekpkgeldvt lpkgkvdidl pegiegkvei sapeigkpdg gfmskmaklp qkmfphgtkg
     4141 gvsvegapel gdiqlkmpea hlgvdikgpe kpkgeldaml pkgkvdidlp egiegkveis
     4201 apeigkpdgg fmskmaklpk kmfshgakgs isvegspefg dvevkmpeah lggdikgpek
     4261 pkgeldvalp kgkvdidipe giegkveisa peigkpsdgg fvskmaklpk kmfphgtkgs
     4321 vsiegapevg dvqvkvpeaq lkmdikgtgk pefegeitwt gkkvavelpe giegtvemst
     4381 pemgrpsdgg fmskvaklpk kvfshgskgg vpfdeetgva gdievkmsea alkvdvkype
     4441 kpesdfsale kkididvpeg fkgkveistp elgkpsdggf lskmsklpkk vlphgskgei
     4501 sagevsevsg evpvelpear lkvgvkgper pdsdvdisvs dkkvtldvtk gfegkvevst
     4561 pdlgkpsdkg fiskvtkfpk kmfphgskgg vsvgevsevt gdvhvelpda hlkvgvkgpe
     4621 rpdsdvdvsm avkkitvdvp egfegklevs tpelarspdg slvskaaklp kklvpsgens
     4681 lssveepsls ahvdvpgsdt tfkvdaklpa mpvtdvdisl sakessadip vgvagkveig
     4741 tpdagkpsdk gfmskmakip kkmfhhgpks sgstdehstv aetvddkalg fevqlqkdvk
     4801 iptkpeadvs vsfhekevtv qlpegregtv elstpglvge hvpdkssqga dvllpgkelp
     4861 tvegqeiiek pggvieviev tahelskpls sgllskvski pkkifprgsk skssdedsps
     4921 pgkrelketp ggivevvevt khdvaihpes thvkqvgtev llepatsetk psgaeglppt
     4981 hitreikevp ggivevvrvt khivgklped kgigepsvrt tdaslpweae lakdpsavik
     5041 revketpegh vevvqvirre asdvpfgsdf pgdlesdgtv srriettvvd gkptvirrlv
     5101 tttsdgirqv tehieqgaed agsaasslfa kmgefakgls sddpsavttr evkqlpggav
     5161 evvsvtkrss sspeteitsq eqlqsstsgs mhfaesavgk pmeakttiir reisgpetiv
     5221 rtttsvvtsa tapgsskpel sadvldkmfg amekprvddr lrdvltaegg yvakrevtpe
     5281 eaellaqlfp eqstgvavyp paglvpeaai ipeyrcmtta egahhdpesk ekvllklgra
     5341 lsqddldaps ptqgeaamtv aglgeavdts dqveepavek lelarreskr ysqefeqdep
     5401 lpkamgfktk pseetvvsst qvkgvhdngt vkhtvttvtk ttiiqeveep agaprpgese
     5461 twasdldtsq vsvsslpede rteptlsssa ttatadaeal sgplartqve ekewtetdpd
     5521 sgevrhiisk tthttvtskk psegaprgdt tsttvshssk dghhgstpsa psecdeaesl
     5581 tffqkkeffe klseqsppke tssarlgstp sldtaltrpa vtlkprprse sltsqgeiie
     5641 esivfaerlr lfqngaqasp lpsasipqvd lvdpapkmeq klqgmaeigd slqqvpsavl
     5701 eglqsteeks dtrllvhtsd adkeafdvrs vqvaedfgdk qfshmrvrmp apeetvskts
     5761 icssdlpgdk psplrdaykg slsasrqess qagkpplpsa lggdsslclv heattdltls
     5821 kagppnqtnk dittfdrqpv reqsptlrss qsqmlasglq mgivagsgil qegikaspqs
     5881 ailetfsssa temtglespe irglpseevh agsaeeakgi dagrigavla aapvailpvr
     5941 diisgkqlem ldiaqvsdep nifegcvelp sqaesqtcpk ektimkteya diqhklssaf
     6001 geslapvesk sgvtqgvpke vlealdrssq eliasklded serdtnilsa tqgsiphdvf
     6061 pdhiestldn saavpmrgay stpsstavcl tperleiqat revaeerqek pevlvaleay
     6121 rkstlrrsge dsydrnleef itvleeyepl pagqlsdwkr aspafepsic hnseitsklp
     6181 pledkkelet vgsildpsen vsellgnhaq sqmlcedtgl ltdelgrtag hyvistaesd
     6241 rgeclptlse qapgkereec lnydsalgvr stsglessll eesheersld larsedeqps
     6301 lvedsmnvpt essvidyqvv ghsgvvkdaq tqpkdsdfdl heisrtgdkt dlplscgddg
     6361 kksaefsktv sgiaadmvik dtdsehlsqd mlhptastvl cegrsdvpys sdedqhkels
     6421 rykevelvip flrgtsssle devedggsfs sgdlqaekia eflttdpdtl nkvvhfpqif
     6481 qdydtsahqe pfvptetemn vekhvgfvld emaacstpcv plksdrqtei eeasrivesv
     6541 vhiasslves lhevnhetai kedicdsala yaagsplers evsetlsqag ppstiekdqr
     6601 espfgvkemy qvlkesrgse driqysvtsf gdplfadegd edqerrrrgs ptaeslddqa
     6661 qiekcshevm mqlldgfsqs dvelaevrte deapdieddv pspeagshee ksvpssqdns
     6721 gdlqpqlste veigsqasga paplseldye lcpdadqlrt sgdeavatrv ppleperdea
     6781 vaesshapsa aadpaasagr naggrerspl leasppeeta ycpeqdispa qeypeyltpd
     6841 mqvrvgvspt eaqpakekpv rysessvess sevgspcpps qggtamafen vafagedvid
     6901 atsspieess spagsrspye vvrdahaeea aqtalveaek slrhavqeev rarpqslvte
     6961 esseqqehqi qpsifneafd hspeesapek kqlsegllgv glltvsdtta rtpesrrgds
     7021 dedeeewadh vmskirgrpi pptppaspra vqsavevqge pweapvveqe rvnvesardm
     7081 saerdagvdv mgdrsatqpq lqefrrtadg krpqlehips eisdedlver eqspthitat
     7141 crgdrkdsss drnsspdsdg pkasgshrrs asgvgaiale avhsqdsggn ekhshesrdk
     7201 gaqeddkite epvdtmfvie eeapadsnrs esfcddader aartagglsp slesppqkkd
     7261 lddgsssgkp daaspvvlrs kskrtdkisd krssrdldtg hsgsetdhsh yysfeltsds
     7321 gktpgasrpt ssefdvnils gqasseyetc atsqdgevsg syataaassh dtsfatarss
     7381 lsgssrssay svdsessghl gsmevsease tivasaheel dsdiesersk svlrepydge
     7441 ipesvirggv ehpflpswqp alkdssptlh gsagarppta egvfspfqst vpsepgrscv
     7501 gwpssslsta esvrspskpe vlngvsaple gqrrlrhndd fmassheeeq lsvsessatq
     7561 sehtwqtsve tmvdaveaqr fscdserhhr qstmqnsvsl pfdeavvaed shreadddra
     7621 tppfqevngp veveyvpeyd dltartlrpv rqqnapdgqt eaqgdvalle asagdastsr
     7681 eqheeenste lreegfyaet fqgdlsseev esaanealll dksacsfyey chakdeyevc
     7741 ntrpavesas lkeaferpat pepvdeiddq edegnlaqgs pnlhgkdyea fghasaeesv
     7801 pcsdkthiaq elsendqykv fsdsgnqssl hdetrsksvs ssleramtdd vrlytskced
     7861 yilqmhqtyk ygvglpktsd ggylpeegmd venvkllesr lqegnggqqd fpydlsyqqe
     7921 yggdgrvehg dlythqevee egyddersrt rgrrrlspis dergstpdyd tlagrktfgk
     7981 gsekddasts slmeferlea emasgtksss vgssdslsgr qhqgrsgags eqdsvsvnsl
     8041 teferlekei veaegvdarl rgevaaklde ideghesqas dpghetavyd esgatlllca
     8101 sadvneepdg ttddaqlgdi eaaqgqevaw srterhfltt rtesghsves vdrqssasta
     8161 tqfdadslre reiddsesnf gpdtpgwmqk ecvdldslhd slhdsehhds lqevpskdkd
     8221 slqlrepsad adslqedhne lqdslyetrd idwdsfrade tsglddtsrs whqfpsqirt
     8281 telsptlsrp assplvvpvv veridtltwv dqdpakacvs dgeslgsrqq ehprndalvs
     8341 stdslepsss anthatyqcd tdsmmsslns aeestmvtst dtlehetlcl asqgkslsle
     8401 gglfsggdvt eeqntrtple ggvsvddmee rfrecllrsa asptneeqve erqtmdekgn
     8461 livtrtvkrh vtseprlhtq tfmgpdaeqr srefvasfaa mepsaerdey egfdaagntv
     8521 rvtqhvvvrp tiktvtftgp darlqmreym rslaggqhss aentptsdsg vgacsslasp
     8581 pplmpdppaa apssacpglq rlsggdqwme aesalddcsp pvtatrvitt rttrtmgpdg
     8641 reqlvtttte esggeepear lrrsmqgvld sfmaepptpp hldeee
//
LOCUS       XP_077503806            8686 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X15 [Amblyomma
            americanum].
ACCESSION   XP_077503806
VERSION     XP_077503806.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647680.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8686
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..8686
                     /product="uncharacterized protein LOC144114148 isoform
                     X15"
                     /calculated_mol_wt=932908
     Region          10..308
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          10..41
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(43,45,49..50,53..55,57..58,62,65,74,76,78,82..83,
                     86..88,90..91,95,98,107,109,111,115..116,119..121,
                     123..124,128,131)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          43..74
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          76..107
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          109..134
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          142..167
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          175..202
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(204,206,210..211,214..216,218..219,223,226,235,237,
                     239,243..244,247..249,251..252,256,259,268,270,272,
                     276..277,280..282,284..285,289,292)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          204..235
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          237..268
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          270..300
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          284..572
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          303..367
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          369..400
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(402,404,408..409,412..414,416..417,421,424,433,435,
                     437,441..442,445..447,449..450,454,457,466,468,470,
                     474..475,478..480,482..483,487,490)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          402..433
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          435..466
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          450..728
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          468..497
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          501..526
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          534..565
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          567..600
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          601..631
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          633..663
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(666,668,672..673,676..678,680..681,685,688,697,699,
                     701,705..706,709..711,713..714,718,721,730,732,734,
                     738..739,742..744,746..747,751,754)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          666..697
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          699..730
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          704..785
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          732..762
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          949..1051
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1274..1406
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1438..1521
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <1835..2086
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          <4449..4709
                     /region_name="YbbR"
                     /note="Cyclic di-AMP synthase regulator CdaR, YbbR domain
                     [Signal transduction mechanisms]; COG4856"
                     /db_xref="CDD:443884"
     Region          <7114..7427
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..8686
                     /gene="LOC144114148"
                     /coded_by="XM_077647680.1:271..26331"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 mvtegeqqtd gnasflraar agnlekvldy lkgsidints nanglnalhl aakeghvnvv
       61 sellkrganv nattkkgnsa lhiaslagqe evvkllvqkq anvnvqsqng ftplymaaqe
      121 nhdgvvrfll anganqslat edgftplava lqqghdkvva vllendargk vrlpalhias
      181 kkddckaaal llhsehnpdv tsksgftplh iaahygnsni asllldkgad vnfpakhqit
      241 plhvaakwgk snmvklllek gakidastrd gltplhcaar sghdqvveql leknapitak
      301 tknglaplhm asqgdhvdsa rillyhkapv ddvtvdylta lhvaahcghv gvakllldrr
      361 adpnaralng ftplhiackk nrikvvelll khgasieatt esgltplhva sfmgcmnivi
      421 yliqhganpd iptvrgetpl hlaaranqtd iirillrnga hvdakarelq talhiasrlg
      481 nadmvglllq hgaavdaptk daytplhvaa regqdevaal lldhgaalaa ptkkgftplh
      541 laakygnlkv aqlllqkdap vdaqgkngvt plhvaahydh vnvallllek gasphsaarn
      601 gytplhvaar kdqmdiassl leygakpgae sragftplhl aaqeghadla allvehgadc
      661 dakakngltp mhlcaqedrv evatilakhg asldpttkag ytplhvachf gqtnmirfll
      721 rqganvnatt shgytplhqa aqqghtliin lllehraapn avtnqgqtal aiaqrlgyis
      781 vvetlkvvte tivttttttv teekyrvvap etmhetfmsd sedeaaednm lgdqsfrylt
      841 ademkslgdd slpidvtrde ritesihitr epghpapltq eeerlsptqa htteaifvgn
      901 yapdnvdlsr tpihagslls wdgdscssay vlsptkrmwr esklkwktfl vsflvdargg
      961 amrgcrhsgv rviipprkaq mpmritcryl rkeklphppp llegeacasr ilevgpagak
     1021 flgpvilevp hfaslrgker eisilrsdng etwkehtlea seeavqevln esfegeelsa
     1081 ledlqtnriv rilttdfpqy favvsrtrqe vhavgpeggl lsstvvpqvq aifpegaltk
     1141 kirvglqaqp ipaelvtkll ghrvavspiv tveprrrkfh kpitltipvp qaatkgminq
     1201 yagdaptlrl lcsitggtnk aqwedvtgst pltfvndcvs ftttvsarfw lmdcrqvsea
     1261 tkfatelyae amhvpfmakf vvfakrqdpm eaqlrvfcmt ddkedktles qehftevaks
     1321 rdvevlekks qylefggnlv pvtksgeqlq lqfqafrenr lpfavrvkdp hqeplgrlaf
     1381 mrqpraarat eppqaplcnl nialpeyeas qnlselvtle kkygfveetg lakpelihra
     1441 dlrlsdiare lgsdwpalaa qldvpeqdva sirtecpsdl aqqallmlrl wmkraggrat
     1501 gnnlekglrm inredivnkc mfnvelvtdd vekavakvhl dqsgfdtfre elgtpkdtsl
     1561 krdasldvsy deqdlmkeae saeetssetg svhekhtgse akgtskaipg vrkgspisaa
     1621 igrivrgssk ekaaprmave etfeeepvlk idsaevtqqa apeagptapp rdskkkskke
     1681 kspsplgpdy slprmrdyht perdftsttd tlivssiked vdaskelatd gkvkkekkke
     1741 kkekspsppg pdytlprlrd tytppsdvta agyaastlpg qgitleqgiv keplerkske
     1801 rslspgvvkt gengslgadp sverqvkqls qgpedtiyek vervvketpg kivemvhikr
     1861 qrsskspegr gspkdknkgl pssevvapdi sgesrsvpse tigaekkqkk hketsptkqg
     1921 kkeakevsea vdkdaelsas dgklpdsgfl skmaklpkkm fprsgkasas deepsslses
     1981 spvakkkagk lpksatlpka kpsktplyei ksvdvevdts kltskgvkgv pakdgeglet
     2041 srdkepdsrf fskmaripkk mfprssktss sedepssase nlpsskqrak kekeisskkk
     2101 ekvvkvkpds kklstsfeve vdrdtlpgag vdinakkpgl qeslesesrk ktdsmqisfp
     2161 qsvkavgskg epmeysldie gmkslqkepq lavvgefpgk pspdielslt sketapefsd
     2221 karghieigt pehgksskgg fmtkmaklpr kmfphgfkgd vsveepsvea esvhvsvpea
     2281 qvklgtkdlk kkgpdmpame ltaslpvgae gsvemsapdl pvsadgglis kvtqfpkmfs
     2341 hdsqdgvtie rppvvgegid vesslpdvql kvkgkafekp etkfdvtlpe rkvtpvlpdg
     2401 aeghievnld emgksyddgf mskmaklpkk mfshgledei avqepsitqh dihvdipklk
     2461 deleiratlp ekpqvdfdva ipgkqvsldl pegveskiev sapdvgkptd ggfmskvvql
     2521 pkkmfphgsk gtvpleeape avgdihakvp eahlkvglkg lekpdfegdi avpgkkgavs
     2581 lpegtegkie vstpetgkps dkgfmskmak lpkkmfphgs kgsvsveevp evdrsvkvkv
     2641 pqgkipeahl kvdikdpekh kdvdialsee kvdidlpegi dknievtmpe vskpseggfi
     2701 skmakfpkkm fphgakgsvs veeapgidvd vkmpetrism dvkglekpkg evdislpkek
     2761 vdidvpkgfd gklemsapev gkpseggfms kmakfpkkmf phgakgsvsv eeapgidvdv
     2821 kvpeahismd vkglekprge vdislpkekv didvpkgfdg klemsapevg kpseggfmsk
     2881 makfpkkmfp hgakgslsve etpgidvdvk mpeadlsmdv kglekpkael diampkgkid
     2941 idmpegvdgk leasapdigk psdggfiskm tkfpkkmfph gakgtvsvea pelgdvqvnm
     3001 peahlgvdik gpekpkgeld vtlpkgkvdi dlpegiegkv eisapeigkp dggfmskmak
     3061 lpkkmfphga kggvsvegsp elgdvevkmp eahlgvdikg pekpkgeldv tlpkgkvdid
     3121 lpegiegkve isapeigkpd ggfmskmakl pkkmfphgak ggvsvegspe lgdvevkmpe
     3181 ahlgvdikgp ekpkgeldvt lpkgkvdidl pegiegkvei sapeigkpdg gfmskmaklp
     3241 kkmfphgakg gvsvegspel gdvevkmpea hlgvdikgpe kpkgeldvtl pkgkvdidlp
     3301 egiegkveis apeigkpdgg fmskmaklpk kmfshgakgg vsvegspelg dvqvkmpeah
     3361 lgvdikgpek pkgeldvtlp kgkvdidlpe giegkveiss peigkpdggf mskmaklpkk
     3421 mfphgakggv svegapelgd vevkmpeahl gvdikgpekp kgeldvtlpk gkvdidlpeg
     3481 iegkveisap eigkpdggfm skmaklpkkm fphgakggvs vegapelgdv evkmpeahlg
     3541 vdikgpekpk geldvtlpkg kvdidlpegf egkveisape igkpdggfms kmaklpkkmf
     3601 phgtkggvsv egapelgdve vkmpeahlgv dikgpekpkg eldvtlpkgk vdidlpegie
     3661 gkveisspei gkpdggfmsk maklpkkmfp hgakggvsve gapelgdvev kmpeahlgvd
     3721 ikgpekpkge ldvtlpkgkv didlpegieg kveisapeig kpdggfmskm aklpkkmfph
     3781 gakggvsveg spelgdvevk mpeahlgvdi kgpekpkgel dvtlpkgkvd idlpegiegk
     3841 leisapeigk pdggfmskma kfpkkmfphg akggvsvega pelgdvqvkm peahlgvdik
     3901 gpekpkgeld vtlpkgkvdi dlpegiegkv eisapeigkp dggfmskmak fpkkmfphga
     3961 kggvsvegap elgdvqvkmp eahlgvdieg pekpkgeldv tlpkgkvdid lpegiegkve
     4021 isapeigkpd ggfmskmakf pkkmfphgtk ggvsvegape lgdvevkmpe ahlgvdikgp
     4081 ekpkgeldvt lpkgkvdidl pegiegkvei sapeigkpdg gfmskmaklp qkmfphgtkg
     4141 gvsvegapel gdiqlkmpea hlgvdikgpe kpkgeldaml pkgkvdidlp egiegkveis
     4201 apeigkpdgg fmskmaklpk kmfshgakgs isvegspefg dvevkmpeah lggdikgpek
     4261 pkgeldvalp kgkvdidipe giegkveisa peigkpsdgg fvskmaklpk kmfphgtkgs
     4321 vsiegapevg dvqvkvpeaq lkmdikgtgk pefegeitwt gkkvavelpe giegtvemst
     4381 pemgrpsdgg fmskvaklpk kvfshgskgg vpfdeetgva gdievkmsea alkvdvkype
     4441 kpesdfsale kkididvpeg fkgkveistp elgkpsdggf lskmsklpkk vlphgskgei
     4501 sagevsevsg evpvelpear lkvgvkgper pdsdvdisvs dkkvtldvtk gfegkvevst
     4561 pdlgkpsdkg fiskvtkfpk kmfphgskgg vsvgevsevt gdvhvelpda hlkvgvkgpe
     4621 rpdsdvdvsm avkkitvdvp egfegklevs tpelarspdg slvskaaklp kklvpsgens
     4681 lssveepsls ahvdvpgsdt tfkvdaklpa mpvtdvdisl sakessadip vgvagkveig
     4741 tpdagkpsdk gfmskmakip kkmfhhgpks sgstdehstv aetvddkalg fevqlqkdvk
     4801 iptkpeadvs vsfhekevtv qlpegregtv elstpglvge hvpdkssqga dvllpgkelp
     4861 tvegqeiiek pggvieviev tahelskpls sgllskvski pkkifprgsk skssdedsps
     4921 pgkrelketp ggivevvevt khdvaihpes thvkqvgtev llepatsetk psgaeglppt
     4981 hitreikevp ggivevvrvt khivgklped kgigepsvrt tdaslpweae lakdpsavik
     5041 revketpegh vevvqvirre asdvpfgsdf pgdlesdgtv srriettvvd gkptvirrlv
     5101 tttsdgirqv tehieqgaed agsaasslfa kmgefakgls sddpsavttr evkqlpggav
     5161 evvsvtkrss sspeteitsq eqlqsstsgs mhfaesavgk pmeakttiir reisgpetiv
     5221 rtttsvvtsa tapgsskpel sadvldkmfg amekprvddr lrdvltaegg yvakrevtpe
     5281 eaellaqlfp eqstgvavyp paglvpeaai ipeyrcmtta egahhdpesk ekvllklgra
     5341 lsqddldaps ptqgeaamtv aglgeavdts dqveepavek lelarreskr ysqefeqdep
     5401 lpkamgfktk pseetvvsst qvkgvhdngt vkhtvttvtk ttiiqeveep agaprpgese
     5461 twasdldtsq vsvsslpede rteptlsssa ttatadaeal sgplartqve ekewtetdpd
     5521 sgevrhiisk tthttvtskk psegaprgdt tsttvshssk dghhgstpsa psecdeaesl
     5581 tffqkkeffe klseqsppke tssarlgstp sldtaltrpa vtlkprprse sltsqgeiie
     5641 esivfaerlr lfqngaqasp lpsasipqvd lvdpapkmeq klqgmaeigd slqqvpsavl
     5701 eglqsteeks dtrllvhtsd adkeafdvrs vqvaedfgdk qfshmrvrmp apeetvskts
     5761 icssdlpgdk psplrdaykg slsasrqess qagkpplpsa lggdsslclv heattdltls
     5821 kagppnqtnk dittfdrqpv reqsptlrss qsqmlasglq mgivagsgil qegikaspqs
     5881 ailetfsssa temtglespe irglpseevh agsaeeakgi dagrigavla aapvailpvr
     5941 diisgkqlem ldiaqvsdep nifegcvelp sqaesqtcpk ektimkteya diqhklssaf
     6001 geslapvesk sgvtqgvpke vlealdrssq eliasklded serdtnilsa tqgsiphdvf
     6061 pdhiestldn saavpmrgay stpsstavcl tperleiqat revaeerqek pevlvaleay
     6121 rkstlrrsge dsydrnleef itvleeyepl pagqlsdwkr aspafepsic hnseitsklp
     6181 pledkkelet vgsildpsen vsellgnhaq sqmlcedtgl ltdelgrtag hyvistaesd
     6241 rgeclptlse qapgkereec lnydsalgvr stsglessll eesheersld larsedeqps
     6301 lvedsmnvpt essvidyqvv ghsgvvkdaq tqpkdsdfdl heisrtgdkt dlplscgddg
     6361 kksaefsktv sgiaadmvik dtdsehlsqd mlhptastvl cegrsdvpys sdedqhkels
     6421 rykevelvip flrgtsssle devedggsfs sgdlqaekia eflttdpdtl nkvvhfpqif
     6481 qdydtsahqe pfvptetemn vekhvgfvld emaacstpcv plksdrqtei eeasrivesv
     6541 vhiasslves lhevnhetai kedicdsala yaagsplers evsetlsqag ppstiekdqr
     6601 espfgvkemy qvlkesrgse driqysvtsf gdplfadegd edqerrrrgs ptaeslddqa
     6661 qiekcshevm mqlldgfsqs dvelaevrte deapdieddv pspeagshee ksvpssqdns
     6721 gdlqpqlste veigsqasga paplseldye lcpdadqlrt sgdeavatrv ppleperdea
     6781 vaesshapsa aadpaasagr naggrerspl leasppeeta ycpeqdispa qeypeyltpd
     6841 mqvrvgvspt eaqpakekpv rysessvess sevgspcpps qggtamafen vafagedvid
     6901 atsspieess spagsrspye vvrdahaeea aqtalveaek slrhavqeev rarpqslvte
     6961 esseqqehqi qpsifneafd hspeesapek kqlsegllgv glltvsdtta rtpesrrgds
     7021 dedeeewadh vmskirgrpi pptppaspra vqsavevqge pweapvveqe rvnvesardm
     7081 saerdagvdv mgdrsatqpq lqefrrtadg krpqlehips eisdedlver eqspthitat
     7141 crgdrkdsss drnsspdsdg pkasgshrrs asgvgaiale avhsqdsggn ekhshesrdk
     7201 gaqeddkite epvdtmfvie eeapadsnrs esfcddader aartagglsp slesppqkkd
     7261 lddgsssgkp daaspvvlrs kskrtdkisd krssrdldtg hsgsetdhsh yysfeltsds
     7321 gktpgasrpt ssefdvnils gqasseyetc atsqdgevsg syataaassh dtsfatarss
     7381 lsgssrssay svdsessghl gsmevsease tivasaheel dsdiesersk svlrepydge
     7441 ipesvirggv ehpflpswqp alkdssptlh gsagarppta egvfspfqst vpsepgrscv
     7501 gwpssslsta esvrspskpe vlngvsaple gqrrlrhndd fmassheeeq lsvsessatq
     7561 sehtwqtsve tmvdaveaqr fscdserhhr qstmqnsvsl pfdeavvaed shreadddra
     7621 tppfqevngp veveyvpeyd dltartlrpv rqqnapdgqt eaqgdvalle asagdastsr
     7681 eqheeenste lreegfyaet fqgdlsseev esaanealll dksacsfyey chakdeyevc
     7741 ntrpavesas lkeaferpat pepvdeiddq edegnlaqgs pnlhgkdyea fghasaeesv
     7801 pcsdkthiaq elsendqykv fsdsgnqssl hdetrsksvs ssleramtdd vrlytskced
     7861 yilqmhqtyk ygvglpktsd ggylpeegmd venvkllesr lqegnggqqd fpydlsyqqe
     7921 yggdgrvehg dlythqevee egyddersrt rgrrrlspis dergstpdyd tlagrktfgk
     7981 gsekddasts slmeferlea emasgtksss vgssdslsgr qhqgrsgags eqdsvsvnsl
     8041 teferlekei veaegvdarl rgevaaklde ideghesqas dpghetavyd esgatlllca
     8101 sadvneepdg ttddaqlgdi eaaqgqevaw srterhfltt rtesghsves vdrqssasta
     8161 tqfdadslre reiddsesnf gpdtpgwmqk ecvdldslhd slhdsehhds lqevpskdkd
     8221 slqlrepsad adslqedhne lqdslyetrd idwdsfrade tsglddtsrs whqfpsqirt
     8281 telsptlsrp assplvvpvv veridtltwv dqdpakacvs dgeslgsrqq ehprndalvs
     8341 stdslepsss anthatyqcd tdsmmsslns aeestmvtst dtlehetlcl asqgkslsle
     8401 gglfsggdvt eeqntrtple ggvsvddmee rfrecllrsa asptneeqve erqtmdekgn
     8461 livtrtvkrh vtseprlhtq tfmgpdaeqr srefvasfaa mepsaerdey egfdaagntv
     8521 rvtqhvvvrp tiktvtftgp darlqmreym rslaggqhss aentptsdsg vgacsslasp
     8581 pplmpdppaa apssacpglq rlsggdqwme aesalddcsp pvtatrvitt rttrtmgpdg
     8641 reqlvtttte esggeepear lrrsmqgvld sfmaepptpp hldeee
//
LOCUS       XP_077503807            8682 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X16 [Amblyomma
            americanum].
ACCESSION   XP_077503807
VERSION     XP_077503807.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647681.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8682
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..8682
                     /product="uncharacterized protein LOC144114148 isoform
                     X16"
                     /calculated_mol_wt=932345
     Region          12..43
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          26..343
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(45,47,51..52,55..57,59..60,64,67,76,78,80,84..85,
                     88..90,92..93,97,100,109,111,113,117..118,121..123,
                     125..126,130,133)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          45..76
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          78..109
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          111..136
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          144..169
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          177..204
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(206,208,212..213,216..218,220..221,225,228,237,239,
                     241,245..246,249..251,253..254,258,261,270,272,274,
                     278..279,282..284,286..287,291,294)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          206..237
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          239..270
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          272..302
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          286..574
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          305..369
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          371..402
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(404,406,410..411,414..416,418..419,423,426,435,437,
                     439,443..444,447..449,451..452,456,459,468,470,472,
                     476..477,480..482,484..485,489,492)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          404..435
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          437..468
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          452..730
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          470..499
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          503..528
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          536..567
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          569..602
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          603..633
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          635..665
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(668,670,674..675,678..680,682..683,687,690,699,701,
                     703,707..708,711..713,715..716,720,723,732,734,736,
                     740..741,744..746,748..749,753,756)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          668..699
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          701..732
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          706..787
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          734..764
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          945..1047
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1270..1402
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1434..1517
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <1831..2082
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          <4445..4705
                     /region_name="YbbR"
                     /note="Cyclic di-AMP synthase regulator CdaR, YbbR domain
                     [Signal transduction mechanisms]; COG4856"
                     /db_xref="CDD:443884"
     Region          <7110..7423
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..8682
                     /gene="LOC144114148"
                     /coded_by="XM_077647681.1:360..26408"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 mgaaqrklak tdgnasflra aragnlekvl dylkgsidin tsnanglnal hlaakeghvn
       61 vvsellkrga nvnattkkgn salhiaslag qeevvkllvq kqanvnvqsq ngftplymaa
      121 qenhdgvvrf llanganqsl atedgftpla valqqghdkv vavllendar gkvrlpalhi
      181 askkddckaa alllhsehnp dvtsksgftp lhiaahygns niasllldkg advnfpakhq
      241 itplhvaakw gksnmvklll ekgakidast rdgltplhca arsghdqvve qlleknapit
      301 aktknglapl hmasqgdhvd sarillyhka pvddvtvdyl talhvaahcg hvgvakllld
      361 rradpnaral ngftplhiac kknrikvvel llkhgasiea ttesgltplh vasfmgcmni
      421 viyliqhgan pdiptvrget plhlaaranq tdiirillrn gahvdakare lqtalhiasr
      481 lgnadmvgll lqhgaavdap tkdaytplhv aaregqdeva allldhgaal aaptkkgftp
      541 lhlaakygnl kvaqlllqkd apvdaqgkng vtplhvaahy dhvnvallll ekgasphsaa
      601 rngytplhva arkdqmdias slleygakpg aesragftpl hlaaqeghad laallvehga
      661 dcdakakngl tpmhlcaqed rvevatilak hgasldpttk agytplhvac hfgqtnmirf
      721 llrqganvna ttshgytplh qaaqqghtli inlllehraa pnavtnqgqt alaiaqrlgy
      781 isvvetlkvv tetivttttt tvteekyrvv apetmhetfm sdsedeaaed nmlgdqsfry
      841 ltademkslg ddslpidvtr deritesihi trepghpapl tqeeerlspt qahtteaifv
      901 gnyapdnvdl srtpihagsl lswdgdscss ayvlsptkrm wrerflvsfl vdarggamrg
      961 crhsgvrvii pprkaqmpmr itcrylrkek lphppplleg eacasrilev gpagakflgp
     1021 vilevphfas lrgkereisi lrsdngetwk ehtleaseea vqevlnesfe geelsaledl
     1081 qtnrivrilt tdfpqyfavv srtrqevhav gpeggllsst vvpqvqaifp egaltkkirv
     1141 glqaqpipae lvtkllghrv avspivtvep rrrkfhkpit ltipvpqaat kgminqyagd
     1201 aptlrllcsi tggtnkaqwe dvtgstpltf vndcvsfttt vsarfwlmdc rqvseatkfa
     1261 telyaeamhv pfmakfvvfa krqdpmeaql rvfcmtddke dktlesqehf tevaksrdve
     1321 vlekksqyle fggnlvpvtk sgeqlqlqfq afrenrlpfa vrvkdphqep lgrlafmrqp
     1381 raarateppq aplcnlnial peyeasqnls elvtlekkyg fveetglakp elihradlrl
     1441 sdiarelgsd wpalaaqldv peqdvasirt ecpsdlaqqa llmlrlwmkr aggratgnnl
     1501 ekglrminre divnkcmfnv elvtddveka vakvhldqsg fdtfreelgt pkdtslkrda
     1561 sldvsydeqd lmkeaesaee tssetgsvhe khtgseakgt skaipgvrkg spisaaigri
     1621 vrgsskekaa prmaveetfe eepvlkidsa evtqqaapea gptapprdsk kkskkeksps
     1681 plgpdyslpr mrdyhtperd ftsttdtliv ssikedvdas kelatdgkvk kekkkekkek
     1741 spsppgpdyt lprlrdtytp psdvtaagya astlpgqgit leqgivkepl erkskersls
     1801 pgvvktgeng slgadpsver qvkqlsqgpe dtiyekverv vketpgkive mvhikrqrss
     1861 kspegrgspk dknkglpsse vvapdisges rsvpsetiga ekkqkkhket sptkqgkkea
     1921 kevseavdkd aelsasdgkl pdsgflskma klpkkmfprs gkasasdeep sslsesspva
     1981 kkkagklpks atlpkakpsk tplyeiksvd vevdtsklts kgvkgvpakd gegletsrdk
     2041 epdsrffskm aripkkmfpr ssktsssede pssasenlps skqrakkeke isskkkekvv
     2101 kvkpdskkls tsfevevdrd tlpgagvdin akkpglqesl esesrkktds mqisfpqsvk
     2161 avgskgepme ysldiegmks lqkepqlavv gefpgkpspd ielsltsket apefsdkarg
     2221 hieigtpehg ksskggfmtk maklprkmfp hgfkgdvsve epsveaesvh vsvpeaqvkl
     2281 gtkdlkkkgp dmpameltas lpvgaegsve msapdlpvsa dggliskvtq fpkmfshdsq
     2341 dgvtierppv vgegidvess lpdvqlkvkg kafekpetkf dvtlperkvt pvlpdgaegh
     2401 ievnldemgk syddgfmskm aklpkkmfsh gledeiavqe psitqhdihv dipklkdele
     2461 iratlpekpq vdfdvaipgk qvsldlpegv eskievsapd vgkptdggfm skvvqlpkkm
     2521 fphgskgtvp leeapeavgd ihakvpeahl kvglkglekp dfegdiavpg kkgavslpeg
     2581 tegkievstp etgkpsdkgf mskmaklpkk mfphgskgsv sveevpevdr svkvkvpqgk
     2641 ipeahlkvdi kdpekhkdvd ialseekvdi dlpegidkni evtmpevskp seggfiskma
     2701 kfpkkmfphg akgsvsveea pgidvdvkmp etrismdvkg lekpkgevdi slpkekvdid
     2761 vpkgfdgkle msapevgkps eggfmskmak fpkkmfphga kgsvsveeap gidvdvkvpe
     2821 ahismdvkgl ekprgevdis lpkekvdidv pkgfdgklem sapevgkpse ggfmskmakf
     2881 pkkmfphgak gslsveetpg idvdvkmpea dlsmdvkgle kpkaeldiam pkgkididmp
     2941 egvdgkleas apdigkpsdg gfiskmtkfp kkmfphgakg tvsveapelg dvqvnmpeah
     3001 lgvdikgpek pkgeldvtlp kgkvdidlpe giegkveisa peigkpdggf mskmaklpkk
     3061 mfphgakggv svegspelgd vevkmpeahl gvdikgpekp kgeldvtlpk gkvdidlpeg
     3121 iegkveisap eigkpdggfm skmaklpkkm fphgakggvs vegspelgdv evkmpeahlg
     3181 vdikgpekpk geldvtlpkg kvdidlpegi egkveisape igkpdggfms kmaklpkkmf
     3241 phgakggvsv egspelgdve vkmpeahlgv dikgpekpkg eldvtlpkgk vdidlpegie
     3301 gkveisapei gkpdggfmsk maklpkkmfs hgakggvsve gspelgdvqv kmpeahlgvd
     3361 ikgpekpkge ldvtlpkgkv didlpegieg kveisspeig kpdggfmskm aklpkkmfph
     3421 gakggvsveg apelgdvevk mpeahlgvdi kgpekpkgel dvtlpkgkvd idlpegiegk
     3481 veisapeigk pdggfmskma klpkkmfphg akggvsvega pelgdvevkm peahlgvdik
     3541 gpekpkgeld vtlpkgkvdi dlpegfegkv eisapeigkp dggfmskmak lpkkmfphgt
     3601 kggvsvegap elgdvevkmp eahlgvdikg pekpkgeldv tlpkgkvdid lpegiegkve
     3661 isspeigkpd ggfmskmakl pkkmfphgak ggvsvegape lgdvevkmpe ahlgvdikgp
     3721 ekpkgeldvt lpkgkvdidl pegiegkvei sapeigkpdg gfmskmaklp kkmfphgakg
     3781 gvsvegspel gdvevkmpea hlgvdikgpe kpkgeldvtl pkgkvdidlp egiegkleis
     3841 apeigkpdgg fmskmakfpk kmfphgakgg vsvegapelg dvqvkmpeah lgvdikgpek
     3901 pkgeldvtlp kgkvdidlpe giegkveisa peigkpdggf mskmakfpkk mfphgakggv
     3961 svegapelgd vqvkmpeahl gvdiegpekp kgeldvtlpk gkvdidlpeg iegkveisap
     4021 eigkpdggfm skmakfpkkm fphgtkggvs vegapelgdv evkmpeahlg vdikgpekpk
     4081 geldvtlpkg kvdidlpegi egkveisape igkpdggfms kmaklpqkmf phgtkggvsv
     4141 egapelgdiq lkmpeahlgv dikgpekpkg eldamlpkgk vdidlpegie gkveisapei
     4201 gkpdggfmsk maklpkkmfs hgakgsisve gspefgdvev kmpeahlggd ikgpekpkge
     4261 ldvalpkgkv didipegieg kveisapeig kpsdggfvsk maklpkkmfp hgtkgsvsie
     4321 gapevgdvqv kvpeaqlkmd ikgtgkpefe geitwtgkkv avelpegieg tvemstpemg
     4381 rpsdggfmsk vaklpkkvfs hgskggvpfd eetgvagdie vkmseaalkv dvkypekpes
     4441 dfsalekkid idvpegfkgk veistpelgk psdggflskm sklpkkvlph gskgeisage
     4501 vsevsgevpv elpearlkvg vkgperpdsd vdisvsdkkv tldvtkgfeg kvevstpdlg
     4561 kpsdkgfisk vtkfpkkmfp hgskggvsvg evsevtgdvh velpdahlkv gvkgperpds
     4621 dvdvsmavkk itvdvpegfe gklevstpel arspdgslvs kaaklpkklv psgenslssv
     4681 eepslsahvd vpgsdttfkv daklpampvt dvdislsake ssadipvgva gkveigtpda
     4741 gkpsdkgfms kmakipkkmf hhgpkssgst dehstvaetv ddkalgfevq lqkdvkiptk
     4801 peadvsvsfh ekevtvqlpe gregtvelst pglvgehvpd kssqgadvll pgkelptveg
     4861 qeiiekpggv ievievtahe lskplssgll skvskipkki fprgskskss dedspspgkr
     4921 elketpggiv evvevtkhdv aihpesthvk qvgtevllep atsetkpsga eglppthitr
     4981 eikevpggiv evvrvtkhiv gklpedkgig epsvrttdas lpweaelakd psavikrevk
     5041 etpeghvevv qvirreasdv pfgsdfpgdl esdgtvsrri ettvvdgkpt virrlvttts
     5101 dgirqvtehi eqgaedagsa asslfakmge fakglssddp savttrevkq lpggavevvs
     5161 vtkrsssspe teitsqeqlq sstsgsmhfa esavgkpmea kttiirreis gpetivrttt
     5221 svvtsatapg sskpelsadv ldkmfgamek prvddrlrdv ltaeggyvak revtpeeael
     5281 laqlfpeqst gvavyppagl vpeaaiipey rcmttaegah hdpeskekvl lklgralsqd
     5341 dldapsptqg eaamtvaglg eavdtsdqve epaveklela rreskrysqe feqdeplpka
     5401 mgfktkpsee tvvsstqvkg vhdngtvkht vttvtkttii qeveepagap rpgesetwas
     5461 dldtsqvsvs slpedertep tlsssattat adaealsgpl artqveekew tetdpdsgev
     5521 rhiiskttht tvtskkpseg aprgdttstt vshsskdghh gstpsapsec deaesltffq
     5581 kkeffeklse qsppketssa rlgstpsldt altrpavtlk prprseslts qgeiieesiv
     5641 faerlrlfqn gaqasplpsa sipqvdlvdp apkmeqklqg maeigdslqq vpsavleglq
     5701 steeksdtrl lvhtsdadke afdvrsvqva edfgdkqfsh mrvrmpapee tvsktsicss
     5761 dlpgdkpspl rdaykgslsa srqessqagk pplpsalggd sslclvheat tdltlskagp
     5821 pnqtnkditt fdrqpvreqs ptlrssqsqm lasglqmgiv agsgilqegi kaspqsaile
     5881 tfsssatemt glespeirgl pseevhagsa eeakgidagr igavlaaapv ailpvrdiis
     5941 gkqlemldia qvsdepnife gcvelpsqae sqtcpkekti mkteyadiqh klssafgesl
     6001 apvesksgvt qgvpkevlea ldrssqelia skldedserd tnilsatqgs iphdvfpdhi
     6061 estldnsaav pmrgaystps stavcltper leiqatreva eerqekpevl valeayrkst
     6121 lrrsgedsyd rnleefitvl eeyeplpagq lsdwkraspa fepsichnse itsklppled
     6181 kkeletvgsi ldpsenvsel lgnhaqsqml cedtglltde lgrtaghyvi staesdrgec
     6241 lptlseqapg kereeclnyd salgvrstsg lesslleesh eersldlars edeqpslved
     6301 smnvptessv idyqvvghsg vvkdaqtqpk dsdfdlheis rtgdktdlpl scgddgkksa
     6361 efsktvsgia admvikdtds ehlsqdmlhp tastvlcegr sdvpyssded qhkelsryke
     6421 velvipflrg tsssledeve dggsfssgdl qaekiaeflt tdpdtlnkvv hfpqifqdyd
     6481 tsahqepfvp tetemnvekh vgfvldemaa cstpcvplks drqteieeas rivesvvhia
     6541 sslveslhev nhetaikedi cdsalayaag splersevse tlsqagppst iekdqrespf
     6601 gvkemyqvlk esrgsedriq ysvtsfgdpl fadegdedqe rrrrgsptae slddqaqiek
     6661 cshevmmqll dgfsqsdvel aevrtedeap dieddvpspe agsheeksvp ssqdnsgdlq
     6721 pqlsteveig sqasgapapl seldyelcpd adqlrtsgde avatrvpple perdeavaes
     6781 shapsaaadp aasagrnagg rersplleas ppeetaycpe qdispaqeyp eyltpdmqvr
     6841 vgvspteaqp akekpvryse ssvesssevg spcppsqggt amafenvafa gedvidatss
     6901 pieessspag srspyevvrd ahaeeaaqta lveaekslrh avqeevrarp qslvteesse
     6961 qqehqiqpsi fneafdhspe esapekkqls egllgvgllt vsdttartpe srrgdsdede
     7021 eewadhvmsk irgrpipptp paspravqsa vevqgepwea pvveqervnv esardmsaer
     7081 dagvdvmgdr satqpqlqef rrtadgkrpq lehipseisd edlvereqsp thitatcrgd
     7141 rkdsssdrns spdsdgpkas gshrrsasgv gaialeavhs qdsggnekhs hesrdkgaqe
     7201 ddkiteepvd tmfvieeeap adsnrsesfc ddaderaart agglspsles ppqkkdlddg
     7261 sssgkpdaas pvvlrskskr tdkisdkrss rdldtghsgs etdhshyysf eltsdsgktp
     7321 gasrptssef dvnilsgqas seyetcatsq dgevsgsyat aaasshdtsf atarsslsgs
     7381 srssaysvds essghlgsme vseasetiva saheeldsdi esersksvlr epydgeipes
     7441 virggvehpf lpswqpalkd ssptlhgsag arpptaegvf spfqstvpse pgrscvgwps
     7501 sslstaesvr spskpevlng vsaplegqrr lrhnddfmas sheeeqlsvs essatqseht
     7561 wqtsvetmvd aveaqrfscd serhhrqstm qnsvslpfde avvaedshre adddratppf
     7621 qevngpveve yvpeyddlta rtlrpvrqqn apdgqteaqg dvalleasag dastsreqhe
     7681 eenstelree gfyaetfqgd lsseevesaa neallldksa csfyeychak deyevcntrp
     7741 avesaslkea ferpatpepv deiddqedeg nlaqgspnlh gkdyeafgha saeesvpcsd
     7801 kthiaqelse ndqykvfsds gnqsslhdet rsksvsssle ramtddvrly tskcedyilq
     7861 mhqtykygvg lpktsdggyl peegmdvenv kllesrlqeg nggqqdfpyd lsyqqeyggd
     7921 grvehgdlyt hqeveeegyd dersrtrgrr rlspisderg stpdydtlag rktfgkgsek
     7981 ddastsslme ferleaemas gtksssvgss dslsgrqhqg rsgagseqds vsvnsltefe
     8041 rlekeiveae gvdarlrgev aakldeideg hesqasdpgh etavydesga tlllcasadv
     8101 neepdgttdd aqlgdieaaq gqevawsrte rhflttrtes ghsvesvdrq ssastatqfd
     8161 adslrereid dsesnfgpdt pgwmqkecvd ldslhdslhd sehhdslqev pskdkdslql
     8221 repsadadsl qedhnelqds lyetrdidwd sfradetsgl ddtsrswhqf psqirttels
     8281 ptlsrpassp lvvpvvveri dtltwvdqdp akacvsdges lgsrqqehpr ndalvsstds
     8341 lepsssanth atyqcdtdsm msslnsaees tmvtstdtle hetlclasqg kslslegglf
     8401 sggdvteeqn trtpleggvs vddmeerfre cllrsaaspt neeqveerqt mdekgnlivt
     8461 rtvkrhvtse prlhtqtfmg pdaeqrsref vasfaameps aerdeyegfd aagntvrvtq
     8521 hvvvrptikt vtftgpdarl qmreymrsla ggqhssaent ptsdsgvgac sslasppplm
     8581 pdppaaapss acpglqrlsg gdqwmeaesa lddcsppvta trvittrttr tmgpdgreql
     8641 vtttteesgg eepearlrrs mqgvldsfma epptpphlde ee
//
LOCUS       XP_077503808            8681 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X17 [Amblyomma
            americanum].
ACCESSION   XP_077503808
VERSION     XP_077503808.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647682.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8681
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..8681
                     /product="uncharacterized protein LOC144114148 isoform
                     X17"
                     /calculated_mol_wt=932215
     Region          12..43
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          26..343
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(45,47,51..52,55..57,59..60,64,67,76,78,80,84..85,
                     88..90,92..93,97,100,109,111,113,117..118,121..123,
                     125..126,130,133)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          45..76
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          78..109
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          111..136
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          144..169
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          177..204
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(206,208,212..213,216..218,220..221,225,228,237,239,
                     241,245..246,249..251,253..254,258,261,270,272,274,
                     278..279,282..284,286..287,291,294)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          206..237
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          239..270
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          272..302
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          286..574
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          305..369
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          371..402
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(404,406,410..411,414..416,418..419,423,426,435,437,
                     439,443..444,447..449,451..452,456,459,468,470,472,
                     476..477,480..482,484..485,489,492)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          404..435
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          437..468
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          452..730
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          470..499
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          503..528
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          536..567
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          569..602
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          603..633
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          635..665
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(668,670,674..675,678..680,682..683,687,690,699,701,
                     703,707..708,711..713,715..716,720,723,732,734,736,
                     740..741,744..746,748..749,753,756)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          668..699
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          701..732
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          706..787
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          734..764
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          945..1047
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1270..1402
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1434..1517
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <1830..2081
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          <4444..4704
                     /region_name="YbbR"
                     /note="Cyclic di-AMP synthase regulator CdaR, YbbR domain
                     [Signal transduction mechanisms]; COG4856"
                     /db_xref="CDD:443884"
     Region          <7109..7422
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..8681
                     /gene="LOC144114148"
                     /coded_by="XM_077647682.1:360..26405"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 mgaaqrklak tdgnasflra aragnlekvl dylkgsidin tsnanglnal hlaakeghvn
       61 vvsellkrga nvnattkkgn salhiaslag qeevvkllvq kqanvnvqsq ngftplymaa
      121 qenhdgvvrf llanganqsl atedgftpla valqqghdkv vavllendar gkvrlpalhi
      181 askkddckaa alllhsehnp dvtsksgftp lhiaahygns niasllldkg advnfpakhq
      241 itplhvaakw gksnmvklll ekgakidast rdgltplhca arsghdqvve qlleknapit
      301 aktknglapl hmasqgdhvd sarillyhka pvddvtvdyl talhvaahcg hvgvakllld
      361 rradpnaral ngftplhiac kknrikvvel llkhgasiea ttesgltplh vasfmgcmni
      421 viyliqhgan pdiptvrget plhlaaranq tdiirillrn gahvdakare lqtalhiasr
      481 lgnadmvgll lqhgaavdap tkdaytplhv aaregqdeva allldhgaal aaptkkgftp
      541 lhlaakygnl kvaqlllqkd apvdaqgkng vtplhvaahy dhvnvallll ekgasphsaa
      601 rngytplhva arkdqmdias slleygakpg aesragftpl hlaaqeghad laallvehga
      661 dcdakakngl tpmhlcaqed rvevatilak hgasldpttk agytplhvac hfgqtnmirf
      721 llrqganvna ttshgytplh qaaqqghtli inlllehraa pnavtnqgqt alaiaqrlgy
      781 isvvetlkvv tetivttttt tvteekyrvv apetmhetfm sdsedeaaed nmlgdqsfry
      841 ltademkslg ddslpidvtr deritesihi trepghpapl tqeeerlspt qahtteaifv
      901 gnyapdnvdl srtpihagsl lswdgdscss ayvlsptkrm wrerflvsfl vdarggamrg
      961 crhsgvrvii pprkaqmpmr itcrylrkek lphppplleg eacasrilev gpagakflgp
     1021 vilevphfas lrgkereisi lrsdngetwk ehtleaseea vqevlnesfe geelsaledl
     1081 qtnrivrilt tdfpqyfavv srtrqevhav gpeggllsst vvpqvqaifp egaltkkirv
     1141 glqaqpipae lvtkllghrv avspivtvep rrrkfhkpit ltipvpqaat kgminqyagd
     1201 aptlrllcsi tggtnkaqwe dvtgstpltf vndcvsfttt vsarfwlmdc rqvseatkfa
     1261 telyaeamhv pfmakfvvfa krqdpmeaql rvfcmtddke dktlesqehf tevaksrdve
     1321 vlekksqyle fggnlvpvtk sgeqlqlqfq afrenrlpfa vrvkdphqep lgrlafmrqp
     1381 raarateppq aplcnlnial peyeasqnls elvtlekkyg fveetglakp elihradlrl
     1441 sdiarelgsd wpalaaqldv peqdvasirt ecpsdlaqqa llmlrlwmkr aggratgnnl
     1501 ekglrminre divnkcmfnv elvtddveka vakvhldqsg fdtfreelgt pkdtslkrda
     1561 sldvsydeqd lmkaesaeet ssetgsvhek htgseakgts kaipgvrkgs pisaaigriv
     1621 rgsskekaap rmaveetfee epvlkidsae vtqqaapeag ptapprdskk kskkekspsp
     1681 lgpdyslprm rdyhtperdf tsttdtlivs sikedvdask elatdgkvkk ekkkekkeks
     1741 psppgpdytl prlrdtytpp sdvtaagyaa stlpgqgitl eqgivkeple rkskerslsp
     1801 gvvktgengs lgadpsverq vkqlsqgped tiyekvervv ketpgkivem vhikrqrssk
     1861 spegrgspkd knkglpssev vapdisgesr svpsetigae kkqkkhkets ptkqgkkeak
     1921 evseavdkda elsasdgklp dsgflskmak lpkkmfprsg kasasdeeps slsesspvak
     1981 kkagklpksa tlpkakpskt plyeiksvdv evdtskltsk gvkgvpakdg egletsrdke
     2041 pdsrffskma ripkkmfprs sktsssedep ssasenlpss kqrakkekei sskkkekvvk
     2101 vkpdskklst sfevevdrdt lpgagvdina kkpglqesle sesrkktdsm qisfpqsvka
     2161 vgskgepmey sldiegmksl qkepqlavvg efpgkpspdi elsltsketa pefsdkargh
     2221 ieigtpehgk sskggfmtkm aklprkmfph gfkgdvsvee psveaesvhv svpeaqvklg
     2281 tkdlkkkgpd mpameltasl pvgaegsvem sapdlpvsad ggliskvtqf pkmfshdsqd
     2341 gvtierppvv gegidvessl pdvqlkvkgk afekpetkfd vtlperkvtp vlpdgaeghi
     2401 evnldemgks yddgfmskma klpkkmfshg ledeiavqep sitqhdihvd ipklkdelei
     2461 ratlpekpqv dfdvaipgkq vsldlpegve skievsapdv gkptdggfms kvvqlpkkmf
     2521 phgskgtvpl eeapeavgdi hakvpeahlk vglkglekpd fegdiavpgk kgavslpegt
     2581 egkievstpe tgkpsdkgfm skmaklpkkm fphgskgsvs veevpevdrs vkvkvpqgki
     2641 peahlkvdik dpekhkdvdi alseekvdid lpegidknie vtmpevskps eggfiskmak
     2701 fpkkmfphga kgsvsveeap gidvdvkmpe trismdvkgl ekpkgevdis lpkekvdidv
     2761 pkgfdgklem sapevgkpse ggfmskmakf pkkmfphgak gsvsveeapg idvdvkvpea
     2821 hismdvkgle kprgevdisl pkekvdidvp kgfdgklems apevgkpseg gfmskmakfp
     2881 kkmfphgakg slsveetpgi dvdvkmpead lsmdvkglek pkaeldiamp kgkididmpe
     2941 gvdgkleasa pdigkpsdgg fiskmtkfpk kmfphgakgt vsveapelgd vqvnmpeahl
     3001 gvdikgpekp kgeldvtlpk gkvdidlpeg iegkveisap eigkpdggfm skmaklpkkm
     3061 fphgakggvs vegspelgdv evkmpeahlg vdikgpekpk geldvtlpkg kvdidlpegi
     3121 egkveisape igkpdggfms kmaklpkkmf phgakggvsv egspelgdve vkmpeahlgv
     3181 dikgpekpkg eldvtlpkgk vdidlpegie gkveisapei gkpdggfmsk maklpkkmfp
     3241 hgakggvsve gspelgdvev kmpeahlgvd ikgpekpkge ldvtlpkgkv didlpegieg
     3301 kveisapeig kpdggfmskm aklpkkmfsh gakggvsveg spelgdvqvk mpeahlgvdi
     3361 kgpekpkgel dvtlpkgkvd idlpegiegk veisspeigk pdggfmskma klpkkmfphg
     3421 akggvsvega pelgdvevkm peahlgvdik gpekpkgeld vtlpkgkvdi dlpegiegkv
     3481 eisapeigkp dggfmskmak lpkkmfphga kggvsvegap elgdvevkmp eahlgvdikg
     3541 pekpkgeldv tlpkgkvdid lpegfegkve isapeigkpd ggfmskmakl pkkmfphgtk
     3601 ggvsvegape lgdvevkmpe ahlgvdikgp ekpkgeldvt lpkgkvdidl pegiegkvei
     3661 sspeigkpdg gfmskmaklp kkmfphgakg gvsvegapel gdvevkmpea hlgvdikgpe
     3721 kpkgeldvtl pkgkvdidlp egiegkveis apeigkpdgg fmskmaklpk kmfphgakgg
     3781 vsvegspelg dvevkmpeah lgvdikgpek pkgeldvtlp kgkvdidlpe giegkleisa
     3841 peigkpdggf mskmakfpkk mfphgakggv svegapelgd vqvkmpeahl gvdikgpekp
     3901 kgeldvtlpk gkvdidlpeg iegkveisap eigkpdggfm skmakfpkkm fphgakggvs
     3961 vegapelgdv qvkmpeahlg vdiegpekpk geldvtlpkg kvdidlpegi egkveisape
     4021 igkpdggfms kmakfpkkmf phgtkggvsv egapelgdve vkmpeahlgv dikgpekpkg
     4081 eldvtlpkgk vdidlpegie gkveisapei gkpdggfmsk maklpqkmfp hgtkggvsve
     4141 gapelgdiql kmpeahlgvd ikgpekpkge ldamlpkgkv didlpegieg kveisapeig
     4201 kpdggfmskm aklpkkmfsh gakgsisveg spefgdvevk mpeahlggdi kgpekpkgel
     4261 dvalpkgkvd idipegiegk veisapeigk psdggfvskm aklpkkmfph gtkgsvsieg
     4321 apevgdvqvk vpeaqlkmdi kgtgkpefeg eitwtgkkva velpegiegt vemstpemgr
     4381 psdggfmskv aklpkkvfsh gskggvpfde etgvagdiev kmseaalkvd vkypekpesd
     4441 fsalekkidi dvpegfkgkv eistpelgkp sdggflskms klpkkvlphg skgeisagev
     4501 sevsgevpve lpearlkvgv kgperpdsdv disvsdkkvt ldvtkgfegk vevstpdlgk
     4561 psdkgfiskv tkfpkkmfph gskggvsvge vsevtgdvhv elpdahlkvg vkgperpdsd
     4621 vdvsmavkki tvdvpegfeg klevstpela rspdgslvsk aaklpkklvp sgenslssve
     4681 epslsahvdv pgsdttfkvd aklpampvtd vdislsakes sadipvgvag kveigtpdag
     4741 kpsdkgfmsk makipkkmfh hgpkssgstd ehstvaetvd dkalgfevql qkdvkiptkp
     4801 eadvsvsfhe kevtvqlpeg regtvelstp glvgehvpdk ssqgadvllp gkelptvegq
     4861 eiiekpggvi evievtahel skplssglls kvskipkkif prgskskssd edspspgkre
     4921 lketpggive vvevtkhdva ihpesthvkq vgtevllepa tsetkpsgae glppthitre
     4981 ikevpggive vvrvtkhivg klpedkgige psvrttdasl pweaelakdp savikrevke
     5041 tpeghvevvq virreasdvp fgsdfpgdle sdgtvsrrie ttvvdgkptv irrlvtttsd
     5101 girqvtehie qgaedagsaa sslfakmgef akglssddps avttrevkql pggavevvsv
     5161 tkrsssspet eitsqeqlqs stsgsmhfae savgkpmeak ttiirreisg petivrttts
     5221 vvtsatapgs skpelsadvl dkmfgamekp rvddrlrdvl taeggyvakr evtpeeaell
     5281 aqlfpeqstg vavyppaglv peaaiipeyr cmttaegahh dpeskekvll klgralsqdd
     5341 ldapsptqge aamtvaglge avdtsdqvee paveklelar reskrysqef eqdeplpkam
     5401 gfktkpseet vvsstqvkgv hdngtvkhtv ttvtkttiiq eveepagapr pgesetwasd
     5461 ldtsqvsvss lpedertept lsssattata daealsgpla rtqveekewt etdpdsgevr
     5521 hiisktthtt vtskkpsega prgdttsttv shsskdghhg stpsapsecd eaesltffqk
     5581 keffeklseq sppketssar lgstpsldta ltrpavtlkp rprsesltsq geiieesivf
     5641 aerlrlfqng aqasplpsas ipqvdlvdpa pkmeqklqgm aeigdslqqv psavleglqs
     5701 teeksdtrll vhtsdadkea fdvrsvqvae dfgdkqfshm rvrmpapeet vsktsicssd
     5761 lpgdkpsplr daykgslsas rqessqagkp plpsalggds slclvheatt dltlskagpp
     5821 nqtnkdittf drqpvreqsp tlrssqsqml asglqmgiva gsgilqegik aspqsailet
     5881 fsssatemtg lespeirglp seevhagsae eakgidagri gavlaaapva ilpvrdiisg
     5941 kqlemldiaq vsdepnifeg cvelpsqaes qtcpkektim kteyadiqhk lssafgesla
     6001 pvesksgvtq gvpkevleal drssqelias kldedserdt nilsatqgsi phdvfpdhie
     6061 stldnsaavp mrgaystpss tavcltperl eiqatrevae erqekpevlv aleayrkstl
     6121 rrsgedsydr nleefitvle eyeplpagql sdwkraspaf epsichnsei tsklppledk
     6181 keletvgsil dpsenvsell gnhaqsqmlc edtglltdel grtaghyvis taesdrgecl
     6241 ptlseqapgk ereeclnyds algvrstsgl esslleeshe ersldlarse deqpslveds
     6301 mnvptessvi dyqvvghsgv vkdaqtqpkd sdfdlheisr tgdktdlpls cgddgkksae
     6361 fsktvsgiaa dmvikdtdse hlsqdmlhpt astvlcegrs dvpyssdedq hkelsrykev
     6421 elvipflrgt sssledeved ggsfssgdlq aekiaefltt dpdtlnkvvh fpqifqdydt
     6481 sahqepfvpt etemnvekhv gfvldemaac stpcvplksd rqteieeasr ivesvvhias
     6541 slveslhevn hetaikedic dsalayaags plersevset lsqagppsti ekdqrespfg
     6601 vkemyqvlke srgsedriqy svtsfgdplf adegdedqer rrrgsptaes lddqaqiekc
     6661 shevmmqlld gfsqsdvela evrtedeapd ieddvpspea gsheeksvps sqdnsgdlqp
     6721 qlsteveigs qasgapapls eldyelcpda dqlrtsgdea vatrvpplep erdeavaess
     6781 hapsaaadpa asagrnaggr ersplleasp peetaycpeq dispaqeype yltpdmqvrv
     6841 gvspteaqpa kekpvryses svesssevgs pcppsqggta mafenvafag edvidatssp
     6901 ieessspags rspyevvrda haeeaaqtal veaekslrha vqeevrarpq slvteesseq
     6961 qehqiqpsif neafdhspee sapekkqlse gllgvglltv sdttartpes rrgdsdedee
     7021 ewadhvmski rgrpipptpp aspravqsav evqgepweap vveqervnve sardmsaerd
     7081 agvdvmgdrs atqpqlqefr rtadgkrpql ehipseisde dlvereqspt hitatcrgdr
     7141 kdsssdrnss pdsdgpkasg shrrsasgvg aialeavhsq dsggnekhsh esrdkgaqed
     7201 dkiteepvdt mfvieeeapa dsnrsesfcd daderaarta gglspslesp pqkkdlddgs
     7261 ssgkpdaasp vvlrskskrt dkisdkrssr dldtghsgse tdhshyysfe ltsdsgktpg
     7321 asrptssefd vnilsgqass eyetcatsqd gevsgsyata aasshdtsfa tarsslsgss
     7381 rssaysvdse ssghlgsmev seasetivas aheeldsdie sersksvlre pydgeipesv
     7441 irggvehpfl pswqpalkds sptlhgsaga rpptaegvfs pfqstvpsep grscvgwpss
     7501 slstaesvrs pskpevlngv saplegqrrl rhnddfmass heeeqlsvse ssatqsehtw
     7561 qtsvetmvda veaqrfscds erhhrqstmq nsvslpfdea vvaedshrea dddratppfq
     7621 evngpvevey vpeyddltar tlrpvrqqna pdgqteaqgd valleasagd astsreqhee
     7681 enstelreeg fyaetfqgdl sseevesaan eallldksac sfyeychakd eyevcntrpa
     7741 vesaslkeaf erpatpepvd eiddqedegn laqgspnlhg kdyeafghas aeesvpcsdk
     7801 thiaqelsen dqykvfsdsg nqsslhdetr sksvsssler amtddvrlyt skcedyilqm
     7861 hqtykygvgl pktsdggylp eegmdvenvk llesrlqegn ggqqdfpydl syqqeyggdg
     7921 rvehgdlyth qeveeegydd ersrtrgrrr lspisdergs tpdydtlagr ktfgkgsekd
     7981 dastsslmef erleaemasg tksssvgssd slsgrqhqgr sgagseqdsv svnsltefer
     8041 lekeiveaeg vdarlrgeva akldeidegh esqasdpghe tavydesgat lllcasadvn
     8101 eepdgttdda qlgdieaaqg qevawsrter hflttrtesg hsvesvdrqs sastatqfda
     8161 dslrereidd sesnfgpdtp gwmqkecvdl dslhdslhds ehhdslqevp skdkdslqlr
     8221 epsadadslq edhnelqdsl yetrdidwds fradetsgld dtsrswhqfp sqirttelsp
     8281 tlsrpasspl vvpvvverid tltwvdqdpa kacvsdgesl gsrqqehprn dalvsstdsl
     8341 epsssantha tyqcdtdsmm sslnsaeest mvtstdtleh etlclasqgk slslegglfs
     8401 ggdvteeqnt rtpleggvsv ddmeerfrec llrsaasptn eeqveerqtm dekgnlivtr
     8461 tvkrhvtsep rlhtqtfmgp daeqrsrefv asfaamepsa erdeyegfda agntvrvtqh
     8521 vvvrptiktv tftgpdarlq mreymrslag gqhssaentp tsdsgvgacs slasppplmp
     8581 dppaaapssa cpglqrlsgg dqwmeaesal ddcsppvtat rvittrttrt mgpdgreqlv
     8641 tttteesgge epearlrrsm qgvldsfmae pptpphldee e
//
LOCUS       XP_077503809            8680 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X18 [Amblyomma
            americanum].
ACCESSION   XP_077503809
VERSION     XP_077503809.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647683.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8680
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..8680
                     /product="uncharacterized protein LOC144114148 isoform
                     X18"
                     /calculated_mol_wt=932192
     Region          10..308
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          10..41
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(43,45,49..50,53..55,57..58,62,65,74,76,78,82..83,
                     86..88,90..91,95,98,107,109,111,115..116,119..121,
                     123..124,128,131)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          43..74
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          76..107
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          109..134
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          142..167
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          175..202
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(204,206,210..211,214..216,218..219,223,226,235,237,
                     239,243..244,247..249,251..252,256,259,268,270,272,
                     276..277,280..282,284..285,289,292)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          204..235
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          237..268
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          270..300
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          284..572
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          303..367
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          369..400
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(402,404,408..409,412..414,416..417,421,424,433,435,
                     437,441..442,445..447,449..450,454,457,466,468,470,
                     474..475,478..480,482..483,487,490)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          402..433
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          435..466
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          450..728
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          468..497
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          501..526
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          534..565
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          567..600
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          601..631
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          633..663
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(666,668,672..673,676..678,680..681,685,688,697,699,
                     701,705..706,709..711,713..714,718,721,730,732,734,
                     738..739,742..744,746..747,751,754)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          666..697
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          699..730
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          704..785
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          732..762
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          943..1045
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1268..1400
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1432..1515
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <1829..2080
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          <4443..4703
                     /region_name="YbbR"
                     /note="Cyclic di-AMP synthase regulator CdaR, YbbR domain
                     [Signal transduction mechanisms]; COG4856"
                     /db_xref="CDD:443884"
     Region          <7108..7421
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..8680
                     /gene="LOC144114148"
                     /coded_by="XM_077647683.1:271..26313"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 mvtegeqqtd gnasflraar agnlekvldy lkgsidints nanglnalhl aakeghvnvv
       61 sellkrganv nattkkgnsa lhiaslagqe evvkllvqkq anvnvqsqng ftplymaaqe
      121 nhdgvvrfll anganqslat edgftplava lqqghdkvva vllendargk vrlpalhias
      181 kkddckaaal llhsehnpdv tsksgftplh iaahygnsni asllldkgad vnfpakhqit
      241 plhvaakwgk snmvklllek gakidastrd gltplhcaar sghdqvveql leknapitak
      301 tknglaplhm asqgdhvdsa rillyhkapv ddvtvdylta lhvaahcghv gvakllldrr
      361 adpnaralng ftplhiackk nrikvvelll khgasieatt esgltplhva sfmgcmnivi
      421 yliqhganpd iptvrgetpl hlaaranqtd iirillrnga hvdakarelq talhiasrlg
      481 nadmvglllq hgaavdaptk daytplhvaa regqdevaal lldhgaalaa ptkkgftplh
      541 laakygnlkv aqlllqkdap vdaqgkngvt plhvaahydh vnvallllek gasphsaarn
      601 gytplhvaar kdqmdiassl leygakpgae sragftplhl aaqeghadla allvehgadc
      661 dakakngltp mhlcaqedrv evatilakhg asldpttkag ytplhvachf gqtnmirfll
      721 rqganvnatt shgytplhqa aqqghtliin lllehraapn avtnqgqtal aiaqrlgyis
      781 vvetlkvvte tivttttttv teekyrvvap etmhetfmsd sedeaaednm lgdqsfrylt
      841 ademkslgdd slpidvtrde ritesihitr epghpapltq eeerlsptqa htteaifvgn
      901 yapdnvdlsr tpihagslls wdgdscssay vlsptkrmwr erflvsflvd arggamrgcr
      961 hsgvrviipp rkaqmpmrit crylrkeklp hpppllegea casrilevgp agakflgpvi
     1021 levphfaslr gkereisilr sdngetwkeh tleaseeavq evlnesfege elsaledlqt
     1081 nrivrilttd fpqyfavvsr trqevhavgp eggllsstvv pqvqaifpeg altkkirvgl
     1141 qaqpipaelv tkllghrvav spivtveprr rkfhkpitlt ipvpqaatkg minqyagdap
     1201 tlrllcsitg gtnkaqwedv tgstpltfvn dcvsftttvs arfwlmdcrq vseatkfate
     1261 lyaeamhvpf makfvvfakr qdpmeaqlrv fcmtddkedk tlesqehfte vaksrdvevl
     1321 ekksqylefg gnlvpvtksg eqlqlqfqaf renrlpfavr vkdphqeplg rlafmrqpra
     1381 arateppqap lcnlnialpe yeasqnlsel vtlekkygfv eetglakpel ihradlrlsd
     1441 iarelgsdwp alaaqldvpe qdvasirtec psdlaqqall mlrlwmkrag gratgnnlek
     1501 glrminredi vnkcmfnvel vtddvekava kvhldqsgfd tfreelgtpk dtslkrdasl
     1561 dvsydeqdlm keaesaeets setgsvhekh tgseakgtsk aipgvrkgsp isaaigrivr
     1621 gsskekaapr maveetfeee pvlkidsaev tqqaapeagp tapprdskkk skkekspspl
     1681 gpdyslprmr dyhtperdft sttdtlivss ikedvdaske latdgkvkke kkkekkeksp
     1741 sppgpdytlp rlrdtytpps dvtaagyaas tlpgqgitle qgivkepler kskerslspg
     1801 vvktgengsl gadpsverqv kqlsqgpedt iyekvervvk etpgkivemv hikrqrssks
     1861 pegrgspkdk nkglpssevv apdisgesrs vpsetigaek kqkkhketsp tkqgkkeake
     1921 vseavdkdae lsasdgklpd sgflskmakl pkkmfprsgk asasdeepss lsesspvakk
     1981 kagklpksat lpkakpsktp lyeiksvdve vdtskltskg vkgvpakdge gletsrdkep
     2041 dsrffskmar ipkkmfprss ktsssedeps sasenlpssk qrakkekeis skkkekvvkv
     2101 kpdskklsts fevevdrdtl pgagvdinak kpglqesles esrkktdsmq isfpqsvkav
     2161 gskgepmeys ldiegmkslq kepqlavvge fpgkpspdie lsltsketap efsdkarghi
     2221 eigtpehgks skggfmtkma klprkmfphg fkgdvsveep sveaesvhvs vpeaqvklgt
     2281 kdlkkkgpdm pameltaslp vgaegsvems apdlpvsadg gliskvtqfp kmfshdsqdg
     2341 vtierppvvg egidvesslp dvqlkvkgka fekpetkfdv tlperkvtpv lpdgaeghie
     2401 vnldemgksy ddgfmskmak lpkkmfshgl edeiavqeps itqhdihvdi pklkdeleir
     2461 atlpekpqvd fdvaipgkqv sldlpegves kievsapdvg kptdggfmsk vvqlpkkmfp
     2521 hgskgtvple eapeavgdih akvpeahlkv glkglekpdf egdiavpgkk gavslpegte
     2581 gkievstpet gkpsdkgfms kmaklpkkmf phgskgsvsv eevpevdrsv kvkvpqgkip
     2641 eahlkvdikd pekhkdvdia lseekvdidl pegidkniev tmpevskpse ggfiskmakf
     2701 pkkmfphgak gsvsveeapg idvdvkmpet rismdvkgle kpkgevdisl pkekvdidvp
     2761 kgfdgklems apevgkpseg gfmskmakfp kkmfphgakg svsveeapgi dvdvkvpeah
     2821 ismdvkglek prgevdislp kekvdidvpk gfdgklemsa pevgkpsegg fmskmakfpk
     2881 kmfphgakgs lsveetpgid vdvkmpeadl smdvkglekp kaeldiampk gkididmpeg
     2941 vdgkleasap digkpsdggf iskmtkfpkk mfphgakgtv sveapelgdv qvnmpeahlg
     3001 vdikgpekpk geldvtlpkg kvdidlpegi egkveisape igkpdggfms kmaklpkkmf
     3061 phgakggvsv egspelgdve vkmpeahlgv dikgpekpkg eldvtlpkgk vdidlpegie
     3121 gkveisapei gkpdggfmsk maklpkkmfp hgakggvsve gspelgdvev kmpeahlgvd
     3181 ikgpekpkge ldvtlpkgkv didlpegieg kveisapeig kpdggfmskm aklpkkmfph
     3241 gakggvsveg spelgdvevk mpeahlgvdi kgpekpkgel dvtlpkgkvd idlpegiegk
     3301 veisapeigk pdggfmskma klpkkmfshg akggvsvegs pelgdvqvkm peahlgvdik
     3361 gpekpkgeld vtlpkgkvdi dlpegiegkv eisspeigkp dggfmskmak lpkkmfphga
     3421 kggvsvegap elgdvevkmp eahlgvdikg pekpkgeldv tlpkgkvdid lpegiegkve
     3481 isapeigkpd ggfmskmakl pkkmfphgak ggvsvegape lgdvevkmpe ahlgvdikgp
     3541 ekpkgeldvt lpkgkvdidl pegfegkvei sapeigkpdg gfmskmaklp kkmfphgtkg
     3601 gvsvegapel gdvevkmpea hlgvdikgpe kpkgeldvtl pkgkvdidlp egiegkveis
     3661 speigkpdgg fmskmaklpk kmfphgakgg vsvegapelg dvevkmpeah lgvdikgpek
     3721 pkgeldvtlp kgkvdidlpe giegkveisa peigkpdggf mskmaklpkk mfphgakggv
     3781 svegspelgd vevkmpeahl gvdikgpekp kgeldvtlpk gkvdidlpeg iegkleisap
     3841 eigkpdggfm skmakfpkkm fphgakggvs vegapelgdv qvkmpeahlg vdikgpekpk
     3901 geldvtlpkg kvdidlpegi egkveisape igkpdggfms kmakfpkkmf phgakggvsv
     3961 egapelgdvq vkmpeahlgv diegpekpkg eldvtlpkgk vdidlpegie gkveisapei
     4021 gkpdggfmsk makfpkkmfp hgtkggvsve gapelgdvev kmpeahlgvd ikgpekpkge
     4081 ldvtlpkgkv didlpegieg kveisapeig kpdggfmskm aklpqkmfph gtkggvsveg
     4141 apelgdiqlk mpeahlgvdi kgpekpkgel damlpkgkvd idlpegiegk veisapeigk
     4201 pdggfmskma klpkkmfshg akgsisvegs pefgdvevkm peahlggdik gpekpkgeld
     4261 valpkgkvdi dipegiegkv eisapeigkp sdggfvskma klpkkmfphg tkgsvsiega
     4321 pevgdvqvkv peaqlkmdik gtgkpefege itwtgkkvav elpegiegtv emstpemgrp
     4381 sdggfmskva klpkkvfshg skggvpfdee tgvagdievk mseaalkvdv kypekpesdf
     4441 salekkidid vpegfkgkve istpelgkps dggflskmsk lpkkvlphgs kgeisagevs
     4501 evsgevpvel pearlkvgvk gperpdsdvd isvsdkkvtl dvtkgfegkv evstpdlgkp
     4561 sdkgfiskvt kfpkkmfphg skggvsvgev sevtgdvhve lpdahlkvgv kgperpdsdv
     4621 dvsmavkkit vdvpegfegk levstpelar spdgslvska aklpkklvps genslssvee
     4681 pslsahvdvp gsdttfkvda klpampvtdv dislsakess adipvgvagk veigtpdagk
     4741 psdkgfmskm akipkkmfhh gpkssgstde hstvaetvdd kalgfevqlq kdvkiptkpe
     4801 advsvsfhek evtvqlpegr egtvelstpg lvgehvpdks sqgadvllpg kelptvegqe
     4861 iiekpggvie vievtahels kplssgllsk vskipkkifp rgskskssde dspspgkrel
     4921 ketpggivev vevtkhdvai hpesthvkqv gtevllepat setkpsgaeg lppthitrei
     4981 kevpggivev vrvtkhivgk lpedkgigep svrttdaslp weaelakdps avikrevket
     5041 peghvevvqv irreasdvpf gsdfpgdles dgtvsrriet tvvdgkptvi rrlvtttsdg
     5101 irqvtehieq gaedagsaas slfakmgefa kglssddpsa vttrevkqlp ggavevvsvt
     5161 krsssspete itsqeqlqss tsgsmhfaes avgkpmeakt tiirreisgp etivrtttsv
     5221 vtsatapgss kpelsadvld kmfgamekpr vddrlrdvlt aeggyvakre vtpeeaella
     5281 qlfpeqstgv avyppaglvp eaaiipeyrc mttaegahhd peskekvllk lgralsqddl
     5341 dapsptqgea amtvaglgea vdtsdqveep aveklelarr eskrysqefe qdeplpkamg
     5401 fktkpseetv vsstqvkgvh dngtvkhtvt tvtkttiiqe veepagaprp gesetwasdl
     5461 dtsqvsvssl pederteptl sssattatad aealsgplar tqveekewte tdpdsgevrh
     5521 iisktthttv tskkpsegap rgdttsttvs hsskdghhgs tpsapsecde aesltffqkk
     5581 effeklseqs ppketssarl gstpsldtal trpavtlkpr prsesltsqg eiieesivfa
     5641 erlrlfqnga qasplpsasi pqvdlvdpap kmeqklqgma eigdslqqvp savleglqst
     5701 eeksdtrllv htsdadkeaf dvrsvqvaed fgdkqfshmr vrmpapeetv sktsicssdl
     5761 pgdkpsplrd aykgslsasr qessqagkpp lpsalggdss lclvheattd ltlskagppn
     5821 qtnkdittfd rqpvreqspt lrssqsqmla sglqmgivag sgilqegika spqsailetf
     5881 sssatemtgl espeirglps eevhagsaee akgidagrig avlaaapvai lpvrdiisgk
     5941 qlemldiaqv sdepnifegc velpsqaesq tcpkektimk teyadiqhkl ssafgeslap
     6001 vesksgvtqg vpkevleald rssqeliask ldedserdtn ilsatqgsip hdvfpdhies
     6061 tldnsaavpm rgaystpsst avcltperle iqatrevaee rqekpevlva leayrkstlr
     6121 rsgedsydrn leefitvlee yeplpagqls dwkraspafe psichnseit sklppledkk
     6181 eletvgsild psenvsellg nhaqsqmlce dtglltdelg rtaghyvist aesdrgeclp
     6241 tlseqapgke reeclnydsa lgvrstsgle sslleeshee rsldlarsed eqpslvedsm
     6301 nvptessvid yqvvghsgvv kdaqtqpkds dfdlheisrt gdktdlplsc gddgkksaef
     6361 sktvsgiaad mvikdtdseh lsqdmlhpta stvlcegrsd vpyssdedqh kelsrykeve
     6421 lvipflrgts ssledevedg gsfssgdlqa ekiaeflttd pdtlnkvvhf pqifqdydts
     6481 ahqepfvpte temnvekhvg fvldemaacs tpcvplksdr qteieeasri vesvvhiass
     6541 lveslhevnh etaikedicd salayaagsp lersevsetl sqagppstie kdqrespfgv
     6601 kemyqvlkes rgsedriqys vtsfgdplfa degdedqerr rrgsptaesl ddqaqiekcs
     6661 hevmmqlldg fsqsdvelae vrtedeapdi eddvpspeag sheeksvpss qdnsgdlqpq
     6721 lsteveigsq asgapaplse ldyelcpdad qlrtsgdeav atrvpplepe rdeavaessh
     6781 apsaaadpaa sagrnaggre rsplleaspp eetaycpeqd ispaqeypey ltpdmqvrvg
     6841 vspteaqpak ekpvrysess vesssevgsp cppsqggtam afenvafage dvidatsspi
     6901 eessspagsr spyevvrdah aeeaaqtalv eaekslrhav qeevrarpqs lvteesseqq
     6961 ehqiqpsifn eafdhspees apekkqlseg llgvglltvs dttartpesr rgdsdedeee
     7021 wadhvmskir grpipptppa spravqsave vqgepweapv veqervnves ardmsaerda
     7081 gvdvmgdrsa tqpqlqefrr tadgkrpqle hipseisded lvereqspth itatcrgdrk
     7141 dsssdrnssp dsdgpkasgs hrrsasgvga ialeavhsqd sggnekhshe srdkgaqedd
     7201 kiteepvdtm fvieeeapad snrsesfcdd aderaartag glspslespp qkkdlddgss
     7261 sgkpdaaspv vlrskskrtd kisdkrssrd ldtghsgset dhshyysfel tsdsgktpga
     7321 srptssefdv nilsgqasse yetcatsqdg evsgsyataa asshdtsfat arsslsgssr
     7381 ssaysvdses sghlgsmevs easetivasa heeldsdies ersksvlrep ydgeipesvi
     7441 rggvehpflp swqpalkdss ptlhgsagar pptaegvfsp fqstvpsepg rscvgwpsss
     7501 lstaesvrsp skpevlngvs aplegqrrlr hnddfmassh eeeqlsvses satqsehtwq
     7561 tsvetmvdav eaqrfscdse rhhrqstmqn svslpfdeav vaedshread ddratppfqe
     7621 vngpveveyv peyddltart lrpvrqqnap dgqteaqgdv alleasagda stsreqheee
     7681 nstelreegf yaetfqgdls seevesaane allldksacs fyeychakde yevcntrpav
     7741 esaslkeafe rpatpepvde iddqedegnl aqgspnlhgk dyeafghasa eesvpcsdkt
     7801 hiaqelsend qykvfsdsgn qsslhdetrs ksvssslera mtddvrlyts kcedyilqmh
     7861 qtykygvglp ktsdggylpe egmdvenvkl lesrlqegng gqqdfpydls yqqeyggdgr
     7921 vehgdlythq eveeegydde rsrtrgrrrl spisdergst pdydtlagrk tfgkgsekdd
     7981 astsslmefe rleaemasgt ksssvgssds lsgrqhqgrs gagseqdsvs vnslteferl
     8041 ekeiveaegv darlrgevaa kldeideghe sqasdpghet avydesgatl llcasadvne
     8101 epdgttddaq lgdieaaqgq evawsrterh flttrtesgh svesvdrqss astatqfdad
     8161 slrereidds esnfgpdtpg wmqkecvdld slhdslhdse hhdslqevps kdkdslqlre
     8221 psadadslqe dhnelqdsly etrdidwdsf radetsgldd tsrswhqfps qirttelspt
     8281 lsrpassplv vpvvveridt ltwvdqdpak acvsdgeslg srqqehprnd alvsstdsle
     8341 psssanthat yqcdtdsmms slnsaeestm vtstdtlehe tlclasqgks lslegglfsg
     8401 gdvteeqntr tpleggvsvd dmeerfrecl lrsaasptne eqveerqtmd ekgnlivtrt
     8461 vkrhvtsepr lhtqtfmgpd aeqrsrefva sfaamepsae rdeyegfdaa gntvrvtqhv
     8521 vvrptiktvt ftgpdarlqm reymrslagg qhssaentpt sdsgvgacss lasppplmpd
     8581 ppaaapssac pglqrlsggd qwmeaesald dcsppvtatr vittrttrtm gpdgreqlvt
     8641 ttteesggee pearlrrsmq gvldsfmaep ptpphldeee
//
LOCUS       XP_077503810            8679 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X19 [Amblyomma
            americanum].
ACCESSION   XP_077503810
VERSION     XP_077503810.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647684.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8679
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..8679
                     /product="uncharacterized protein LOC144114148 isoform
                     X19"
                     /calculated_mol_wt=932063
     Region          10..308
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          10..41
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(43,45,49..50,53..55,57..58,62,65,74,76,78,82..83,
                     86..88,90..91,95,98,107,109,111,115..116,119..121,
                     123..124,128,131)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          43..74
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          76..107
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          109..134
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          142..167
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          175..202
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(204,206,210..211,214..216,218..219,223,226,235,237,
                     239,243..244,247..249,251..252,256,259,268,270,272,
                     276..277,280..282,284..285,289,292)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          204..235
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          237..268
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          270..300
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          284..572
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          303..367
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          369..400
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(402,404,408..409,412..414,416..417,421,424,433,435,
                     437,441..442,445..447,449..450,454,457,466,468,470,
                     474..475,478..480,482..483,487,490)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          402..433
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          435..466
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          450..728
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          468..497
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          501..526
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          534..565
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          567..600
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          601..631
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          633..663
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(666,668,672..673,676..678,680..681,685,688,697,699,
                     701,705..706,709..711,713..714,718,721,730,732,734,
                     738..739,742..744,746..747,751,754)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          666..697
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          699..730
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          704..785
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          732..762
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          943..1045
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1268..1400
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1432..1515
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <1828..2079
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          <4442..4702
                     /region_name="YbbR"
                     /note="Cyclic di-AMP synthase regulator CdaR, YbbR domain
                     [Signal transduction mechanisms]; COG4856"
                     /db_xref="CDD:443884"
     Region          <7107..7420
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..8679
                     /gene="LOC144114148"
                     /coded_by="XM_077647684.1:271..26310"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 mvtegeqqtd gnasflraar agnlekvldy lkgsidints nanglnalhl aakeghvnvv
       61 sellkrganv nattkkgnsa lhiaslagqe evvkllvqkq anvnvqsqng ftplymaaqe
      121 nhdgvvrfll anganqslat edgftplava lqqghdkvva vllendargk vrlpalhias
      181 kkddckaaal llhsehnpdv tsksgftplh iaahygnsni asllldkgad vnfpakhqit
      241 plhvaakwgk snmvklllek gakidastrd gltplhcaar sghdqvveql leknapitak
      301 tknglaplhm asqgdhvdsa rillyhkapv ddvtvdylta lhvaahcghv gvakllldrr
      361 adpnaralng ftplhiackk nrikvvelll khgasieatt esgltplhva sfmgcmnivi
      421 yliqhganpd iptvrgetpl hlaaranqtd iirillrnga hvdakarelq talhiasrlg
      481 nadmvglllq hgaavdaptk daytplhvaa regqdevaal lldhgaalaa ptkkgftplh
      541 laakygnlkv aqlllqkdap vdaqgkngvt plhvaahydh vnvallllek gasphsaarn
      601 gytplhvaar kdqmdiassl leygakpgae sragftplhl aaqeghadla allvehgadc
      661 dakakngltp mhlcaqedrv evatilakhg asldpttkag ytplhvachf gqtnmirfll
      721 rqganvnatt shgytplhqa aqqghtliin lllehraapn avtnqgqtal aiaqrlgyis
      781 vvetlkvvte tivttttttv teekyrvvap etmhetfmsd sedeaaednm lgdqsfrylt
      841 ademkslgdd slpidvtrde ritesihitr epghpapltq eeerlsptqa htteaifvgn
      901 yapdnvdlsr tpihagslls wdgdscssay vlsptkrmwr erflvsflvd arggamrgcr
      961 hsgvrviipp rkaqmpmrit crylrkeklp hpppllegea casrilevgp agakflgpvi
     1021 levphfaslr gkereisilr sdngetwkeh tleaseeavq evlnesfege elsaledlqt
     1081 nrivrilttd fpqyfavvsr trqevhavgp eggllsstvv pqvqaifpeg altkkirvgl
     1141 qaqpipaelv tkllghrvav spivtveprr rkfhkpitlt ipvpqaatkg minqyagdap
     1201 tlrllcsitg gtnkaqwedv tgstpltfvn dcvsftttvs arfwlmdcrq vseatkfate
     1261 lyaeamhvpf makfvvfakr qdpmeaqlrv fcmtddkedk tlesqehfte vaksrdvevl
     1321 ekksqylefg gnlvpvtksg eqlqlqfqaf renrlpfavr vkdphqeplg rlafmrqpra
     1381 arateppqap lcnlnialpe yeasqnlsel vtlekkygfv eetglakpel ihradlrlsd
     1441 iarelgsdwp alaaqldvpe qdvasirtec psdlaqqall mlrlwmkrag gratgnnlek
     1501 glrminredi vnkcmfnvel vtddvekava kvhldqsgfd tfreelgtpk dtslkrdasl
     1561 dvsydeqdlm kaesaeetss etgsvhekht gseakgtska ipgvrkgspi saaigrivrg
     1621 sskekaaprm aveetfeeep vlkidsaevt qqaapeagpt apprdskkks kkekspsplg
     1681 pdyslprmrd yhtperdfts ttdtlivssi kedvdaskel atdgkvkkek kkekkeksps
     1741 ppgpdytlpr lrdtytppsd vtaagyaast lpgqgitleq givkeplerk skerslspgv
     1801 vktgengslg adpsverqvk qlsqgpedti yekvervvke tpgkivemvh ikrqrssksp
     1861 egrgspkdkn kglpssevva pdisgesrsv psetigaekk qkkhketspt kqgkkeakev
     1921 seavdkdael sasdgklpds gflskmaklp kkmfprsgka sasdeepssl sesspvakkk
     1981 agklpksatl pkakpsktpl yeiksvdvev dtskltskgv kgvpakdgeg letsrdkepd
     2041 srffskmari pkkmfprssk tsssedepss asenlpsskq rakkekeiss kkkekvvkvk
     2101 pdskklstsf evevdrdtlp gagvdinakk pglqeslese srkktdsmqi sfpqsvkavg
     2161 skgepmeysl diegmkslqk epqlavvgef pgkpspdiel sltsketape fsdkarghie
     2221 igtpehgkss kggfmtkmak lprkmfphgf kgdvsveeps veaesvhvsv peaqvklgtk
     2281 dlkkkgpdmp ameltaslpv gaegsvemsa pdlpvsadgg liskvtqfpk mfshdsqdgv
     2341 tierppvvge gidvesslpd vqlkvkgkaf ekpetkfdvt lperkvtpvl pdgaeghiev
     2401 nldemgksyd dgfmskmakl pkkmfshgle deiavqepsi tqhdihvdip klkdeleira
     2461 tlpekpqvdf dvaipgkqvs ldlpegvesk ievsapdvgk ptdggfmskv vqlpkkmfph
     2521 gskgtvplee apeavgdiha kvpeahlkvg lkglekpdfe gdiavpgkkg avslpegteg
     2581 kievstpetg kpsdkgfmsk maklpkkmfp hgskgsvsve evpevdrsvk vkvpqgkipe
     2641 ahlkvdikdp ekhkdvdial seekvdidlp egidknievt mpevskpseg gfiskmakfp
     2701 kkmfphgakg svsveeapgi dvdvkmpetr ismdvkglek pkgevdislp kekvdidvpk
     2761 gfdgklemsa pevgkpsegg fmskmakfpk kmfphgakgs vsveeapgid vdvkvpeahi
     2821 smdvkglekp rgevdislpk ekvdidvpkg fdgklemsap evgkpseggf mskmakfpkk
     2881 mfphgakgsl sveetpgidv dvkmpeadls mdvkglekpk aeldiampkg kididmpegv
     2941 dgkleasapd igkpsdggfi skmtkfpkkm fphgakgtvs veapelgdvq vnmpeahlgv
     3001 dikgpekpkg eldvtlpkgk vdidlpegie gkveisapei gkpdggfmsk maklpkkmfp
     3061 hgakggvsve gspelgdvev kmpeahlgvd ikgpekpkge ldvtlpkgkv didlpegieg
     3121 kveisapeig kpdggfmskm aklpkkmfph gakggvsveg spelgdvevk mpeahlgvdi
     3181 kgpekpkgel dvtlpkgkvd idlpegiegk veisapeigk pdggfmskma klpkkmfphg
     3241 akggvsvegs pelgdvevkm peahlgvdik gpekpkgeld vtlpkgkvdi dlpegiegkv
     3301 eisapeigkp dggfmskmak lpkkmfshga kggvsvegsp elgdvqvkmp eahlgvdikg
     3361 pekpkgeldv tlpkgkvdid lpegiegkve isspeigkpd ggfmskmakl pkkmfphgak
     3421 ggvsvegape lgdvevkmpe ahlgvdikgp ekpkgeldvt lpkgkvdidl pegiegkvei
     3481 sapeigkpdg gfmskmaklp kkmfphgakg gvsvegapel gdvevkmpea hlgvdikgpe
     3541 kpkgeldvtl pkgkvdidlp egfegkveis apeigkpdgg fmskmaklpk kmfphgtkgg
     3601 vsvegapelg dvevkmpeah lgvdikgpek pkgeldvtlp kgkvdidlpe giegkveiss
     3661 peigkpdggf mskmaklpkk mfphgakggv svegapelgd vevkmpeahl gvdikgpekp
     3721 kgeldvtlpk gkvdidlpeg iegkveisap eigkpdggfm skmaklpkkm fphgakggvs
     3781 vegspelgdv evkmpeahlg vdikgpekpk geldvtlpkg kvdidlpegi egkleisape
     3841 igkpdggfms kmakfpkkmf phgakggvsv egapelgdvq vkmpeahlgv dikgpekpkg
     3901 eldvtlpkgk vdidlpegie gkveisapei gkpdggfmsk makfpkkmfp hgakggvsve
     3961 gapelgdvqv kmpeahlgvd iegpekpkge ldvtlpkgkv didlpegieg kveisapeig
     4021 kpdggfmskm akfpkkmfph gtkggvsveg apelgdvevk mpeahlgvdi kgpekpkgel
     4081 dvtlpkgkvd idlpegiegk veisapeigk pdggfmskma klpqkmfphg tkggvsvega
     4141 pelgdiqlkm peahlgvdik gpekpkgeld amlpkgkvdi dlpegiegkv eisapeigkp
     4201 dggfmskmak lpkkmfshga kgsisvegsp efgdvevkmp eahlggdikg pekpkgeldv
     4261 alpkgkvdid ipegiegkve isapeigkps dggfvskmak lpkkmfphgt kgsvsiegap
     4321 evgdvqvkvp eaqlkmdikg tgkpefegei twtgkkvave lpegiegtve mstpemgrps
     4381 dggfmskvak lpkkvfshgs kggvpfdeet gvagdievkm seaalkvdvk ypekpesdfs
     4441 alekkididv pegfkgkvei stpelgkpsd ggflskmskl pkkvlphgsk geisagevse
     4501 vsgevpvelp earlkvgvkg perpdsdvdi svsdkkvtld vtkgfegkve vstpdlgkps
     4561 dkgfiskvtk fpkkmfphgs kggvsvgevs evtgdvhvel pdahlkvgvk gperpdsdvd
     4621 vsmavkkitv dvpegfegkl evstpelars pdgslvskaa klpkklvpsg enslssveep
     4681 slsahvdvpg sdttfkvdak lpampvtdvd islsakessa dipvgvagkv eigtpdagkp
     4741 sdkgfmskma kipkkmfhhg pkssgstdeh stvaetvddk algfevqlqk dvkiptkpea
     4801 dvsvsfheke vtvqlpegre gtvelstpgl vgehvpdkss qgadvllpgk elptvegqei
     4861 iekpggviev ievtahelsk plssgllskv skipkkifpr gskskssded spspgkrelk
     4921 etpggivevv evtkhdvaih pesthvkqvg tevllepats etkpsgaegl ppthitreik
     4981 evpggivevv rvtkhivgkl pedkgigeps vrttdaslpw eaelakdpsa vikrevketp
     5041 eghvevvqvi rreasdvpfg sdfpgdlesd gtvsrriett vvdgkptvir rlvtttsdgi
     5101 rqvtehieqg aedagsaass lfakmgefak glssddpsav ttrevkqlpg gavevvsvtk
     5161 rsssspetei tsqeqlqsst sgsmhfaesa vgkpmeaktt iirreisgpe tivrtttsvv
     5221 tsatapgssk pelsadvldk mfgamekprv ddrlrdvlta eggyvakrev tpeeaellaq
     5281 lfpeqstgva vyppaglvpe aaiipeyrcm ttaegahhdp eskekvllkl gralsqddld
     5341 apsptqgeaa mtvaglgeav dtsdqveepa veklelarre skrysqefeq deplpkamgf
     5401 ktkpseetvv sstqvkgvhd ngtvkhtvtt vtkttiiqev eepagaprpg esetwasdld
     5461 tsqvsvsslp ederteptls ssattatada ealsgplart qveekewtet dpdsgevrhi
     5521 isktthttvt skkpsegapr gdttsttvsh sskdghhgst psapsecdea esltffqkke
     5581 ffeklseqsp pketssarlg stpsldtalt rpavtlkprp rsesltsqge iieesivfae
     5641 rlrlfqngaq asplpsasip qvdlvdpapk meqklqgmae igdslqqvps avleglqste
     5701 eksdtrllvh tsdadkeafd vrsvqvaedf gdkqfshmrv rmpapeetvs ktsicssdlp
     5761 gdkpsplrda ykgslsasrq essqagkppl psalggdssl clvheattdl tlskagppnq
     5821 tnkdittfdr qpvreqsptl rssqsqmlas glqmgivags gilqegikas pqsailetfs
     5881 ssatemtgle speirglpse evhagsaeea kgidagriga vlaaapvail pvrdiisgkq
     5941 lemldiaqvs depnifegcv elpsqaesqt cpkektimkt eyadiqhkls safgeslapv
     6001 esksgvtqgv pkevlealdr ssqeliaskl dedserdtni lsatqgsiph dvfpdhiest
     6061 ldnsaavpmr gaystpssta vcltperlei qatrevaeer qekpevlval eayrkstlrr
     6121 sgedsydrnl eefitvleey eplpagqlsd wkraspafep sichnseits klppledkke
     6181 letvgsildp senvsellgn haqsqmlced tglltdelgr taghyvista esdrgeclpt
     6241 lseqapgker eeclnydsal gvrstsgles slleesheer sldlarsede qpslvedsmn
     6301 vptessvidy qvvghsgvvk daqtqpkdsd fdlheisrtg dktdlplscg ddgkksaefs
     6361 ktvsgiaadm vikdtdsehl sqdmlhptas tvlcegrsdv pyssdedqhk elsrykevel
     6421 vipflrgtss sledevedgg sfssgdlqae kiaeflttdp dtlnkvvhfp qifqdydtsa
     6481 hqepfvptet emnvekhvgf vldemaacst pcvplksdrq teieeasriv esvvhiassl
     6541 veslhevnhe taikedicds alayaagspl ersevsetls qagppstiek dqrespfgvk
     6601 emyqvlkesr gsedriqysv tsfgdplfad egdedqerrr rgsptaesld dqaqiekcsh
     6661 evmmqlldgf sqsdvelaev rtedeapdie ddvpspeags heeksvpssq dnsgdlqpql
     6721 steveigsqa sgapaplsel dyelcpdadq lrtsgdeava trvppleper deavaessha
     6781 psaaadpaas agrnaggrer splleasppe etaycpeqdi spaqeypeyl tpdmqvrvgv
     6841 spteaqpake kpvrysessv esssevgspc ppsqggtama fenvafaged vidatsspie
     6901 essspagsrs pyevvrdaha eeaaqtalve aekslrhavq eevrarpqsl vteesseqqe
     6961 hqiqpsifne afdhspeesa pekkqlsegl lgvglltvsd ttartpesrr gdsdedeeew
     7021 adhvmskirg rpipptppas pravqsavev qgepweapvv eqervnvesa rdmsaerdag
     7081 vdvmgdrsat qpqlqefrrt adgkrpqleh ipseisdedl vereqspthi tatcrgdrkd
     7141 sssdrnsspd sdgpkasgsh rrsasgvgai aleavhsqds ggnekhshes rdkgaqeddk
     7201 iteepvdtmf vieeeapads nrsesfcdda deraartagg lspslesppq kkdlddgsss
     7261 gkpdaaspvv lrskskrtdk isdkrssrdl dtghsgsetd hshyysfelt sdsgktpgas
     7321 rptssefdvn ilsgqassey etcatsqdge vsgsyataaa sshdtsfata rsslsgssrs
     7381 saysvdsess ghlgsmevse asetivasah eeldsdiese rsksvlrepy dgeipesvir
     7441 ggvehpflps wqpalkdssp tlhgsagarp ptaegvfspf qstvpsepgr scvgwpsssl
     7501 staesvrsps kpevlngvsa plegqrrlrh nddfmasshe eeqlsvsess atqsehtwqt
     7561 svetmvdave aqrfscdser hhrqstmqns vslpfdeavv aedshreadd dratppfqev
     7621 ngpveveyvp eyddltartl rpvrqqnapd gqteaqgdva lleasagdas tsreqheeen
     7681 stelreegfy aetfqgdlss eevesaanea llldksacsf yeychakdey evcntrpave
     7741 saslkeafer patpepvdei ddqedegnla qgspnlhgkd yeafghasae esvpcsdkth
     7801 iaqelsendq ykvfsdsgnq sslhdetrsk svsssleram tddvrlytsk cedyilqmhq
     7861 tykygvglpk tsdggylpee gmdvenvkll esrlqegngg qqdfpydlsy qqeyggdgrv
     7921 ehgdlythqe veeegydder srtrgrrrls pisdergstp dydtlagrkt fgkgsekdda
     7981 stsslmefer leaemasgtk sssvgssdsl sgrqhqgrsg agseqdsvsv nslteferle
     8041 keiveaegvd arlrgevaak ldeideghes qasdpgheta vydesgatll lcasadvnee
     8101 pdgttddaql gdieaaqgqe vawsrterhf lttrtesghs vesvdrqssa statqfdads
     8161 lrereiddse snfgpdtpgw mqkecvdlds lhdslhdseh hdslqevpsk dkdslqlrep
     8221 sadadslqed hnelqdslye trdidwdsfr adetsglddt srswhqfpsq irttelsptl
     8281 srpassplvv pvvveridtl twvdqdpaka cvsdgeslgs rqqehprnda lvsstdslep
     8341 sssanthaty qcdtdsmmss lnsaeestmv tstdtlehet lclasqgksl slegglfsgg
     8401 dvteeqntrt pleggvsvdd meerfrecll rsaasptnee qveerqtmde kgnlivtrtv
     8461 krhvtseprl htqtfmgpda eqrsrefvas faamepsaer deyegfdaag ntvrvtqhvv
     8521 vrptiktvtf tgpdarlqmr eymrslaggq hssaentpts dsgvgacssl asppplmpdp
     8581 paaapssacp glqrlsggdq wmeaesaldd csppvtatrv ittrttrtmg pdgreqlvtt
     8641 tteesggeep earlrrsmqg vldsfmaepp tpphldeee
//
LOCUS       XP_077503812            8668 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X20 [Amblyomma
            americanum].
ACCESSION   XP_077503812
VERSION     XP_077503812.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647686.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8668
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..8668
                     /product="uncharacterized protein LOC144114148 isoform
                     X20"
                     /calculated_mol_wt=930433
     Region          24..55
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          38..355
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(57,59,63..64,67..69,71..72,76,79,88,90,92,96..97,
                     100..102,104..105,109,112,121,123,125,129..130,133..135,
                     137..138,142,145)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          57..88
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          90..121
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          123..148
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          156..181
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          189..216
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(218,220,224..225,228..230,232..233,237,240,249,251,
                     253,257..258,261..263,265..266,270,273,282,284,286,
                     290..291,294..296,298..299,303,306)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          218..249
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          251..282
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          284..314
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          298..586
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          317..381
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          383..414
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(416,418,422..423,426..428,430..431,435,438,447,449,
                     451,455..456,459..461,463..464,468,471,480,482,484,
                     488..489,492..494,496..497,501,504)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          416..447
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          449..480
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          464..742
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          482..511
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          515..540
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          548..579
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          581..614
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          615..645
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          647..677
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(680,682,686..687,690..692,694..695,699,702,711,713,
                     715,719..720,723..725,727..728,732,735,744,746,748,
                     752..753,756..758,760..761,765,768)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          680..711
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          713..744
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          718..799
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          746..776
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          930..1033
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1256..1388
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1420..1503
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <1817..2068
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          <4431..4691
                     /region_name="YbbR"
                     /note="Cyclic di-AMP synthase regulator CdaR, YbbR domain
                     [Signal transduction mechanisms]; COG4856"
                     /db_xref="CDD:443884"
     Region          <7096..7409
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..8668
                     /gene="LOC144114148"
                     /coded_by="XM_077647686.1:257..26263"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 maitahdapp gspngqipds aqtdgnasfl raaragnlek vldylkgsid intsnangln
       61 alhlaakegh vnvvsellkr ganvnattkk gnsalhiasl agqeevvkll vqkqanvnvq
      121 sqngftplym aaqenhdgvv rfllanganq slatedgftp lavalqqghd kvvavllend
      181 argkvrlpal hiaskkddck aaalllhseh npdvtsksgf tplhiaahyg nsniasllld
      241 kgadvnfpak hqitplhvaa kwgksnmvkl llekgakida strdgltplh caarsghdqv
      301 veqlleknap itaktkngla plhmasqgdh vdsarillyh kapvddvtvd yltalhvaah
      361 cghvgvakll ldrradpnar alngftplhi ackknrikvv elllkhgasi eattesgltp
      421 lhvasfmgcm niviyliqhg anpdiptvrg etplhlaara nqtdiirill rngahvdaka
      481 relqtalhia srlgnadmvg lllqhgaavd aptkdaytpl hvaaregqde vaallldhga
      541 alaaptkkgf tplhlaakyg nlkvaqlllq kdapvdaqgk ngvtplhvaa hydhvnvall
      601 llekgasphs aarngytplh vaarkdqmdi asslleygak pgaesragft plhlaaqegh
      661 adlaallveh gadcdakakn gltpmhlcaq edrvevatil akhgasldpt tkagytplhv
      721 achfgqtnmi rfllrqganv nattshgytp lhqaaqqght liinlllehr aapnavtnqg
      781 qtalaiaqrl gyisvvetlk vvtetivttt tttvteekyr vvapetmhet fmsdsedeaa
      841 ednmlgdqsf ryltademks lgddslpidv trderitesi hitrepghpa pltqeeerls
      901 ptqahtteai fvgnyapdnv dlsrtpihag flvsflvdar ggamrgcrhs gvrviipprk
      961 aqmpmritcr ylrkeklphp ppllegeaca srilevgpag akflgpvile vphfaslrgk
     1021 ereisilrsd ngetwkehtl easeeavqev lnesfegeel saledlqtnr ivrilttdfp
     1081 qyfavvsrtr qevhavgpeg gllsstvvpq vqaifpegal tkkirvglqa qpipaelvtk
     1141 llghrvavsp ivtveprrrk fhkpitltip vpqaatkgmi nqyagdaptl rllcsitggt
     1201 nkaqwedvtg stpltfvndc vsftttvsar fwlmdcrqvs eatkfately aeamhvpfma
     1261 kfvvfakrqd pmeaqlrvfc mtddkedktl esqehfteva ksrdvevlek ksqylefggn
     1321 lvpvtksgeq lqlqfqafre nrlpfavrvk dphqeplgrl afmrqpraar ateppqaplc
     1381 nlnialpeye asqnlselvt lekkygfvee tglakpelih radlrlsdia relgsdwpal
     1441 aaqldvpeqd vasirtecps dlaqqallml rlwmkraggr atgnnlekgl rminredivn
     1501 kcmfnvelvt ddvekavakv hldqsgfdtf reelgtpkdt slkrdasldv sydeqdlmke
     1561 aesaeetsse tgsvhekhtg seakgtskai pgvrkgspis aaigrivrgs skekaaprma
     1621 veetfeeepv lkidsaevtq qaapeagpta pprdskkksk kekspsplgp dyslprmrdy
     1681 htperdftst tdtlivssik edvdaskela tdgkvkkekk kekkekspsp pgpdytlprl
     1741 rdtytppsdv taagyaastl pgqgitleqg ivkeplerks kerslspgvv ktgengslga
     1801 dpsverqvkq lsqgpedtiy ekvervvket pgkivemvhi krqrsskspe grgspkdknk
     1861 glpssevvap disgesrsvp setigaekkq kkhketsptk qgkkeakevs eavdkdaels
     1921 asdgklpdsg flskmaklpk kmfprsgkas asdeepssls esspvakkka gklpksatlp
     1981 kakpsktply eiksvdvevd tskltskgvk gvpakdgegl etsrdkepds rffskmarip
     2041 kkmfprsskt sssedepssa senlpsskqr akkekeissk kkekvvkvkp dskklstsfe
     2101 vevdrdtlpg agvdinakkp glqesleses rkktdsmqis fpqsvkavgs kgepmeysld
     2161 iegmkslqke pqlavvgefp gkpspdiels ltsketapef sdkarghiei gtpehgkssk
     2221 ggfmtkmakl prkmfphgfk gdvsveepsv eaesvhvsvp eaqvklgtkd lkkkgpdmpa
     2281 meltaslpvg aegsvemsap dlpvsadggl iskvtqfpkm fshdsqdgvt ierppvvgeg
     2341 idvesslpdv qlkvkgkafe kpetkfdvtl perkvtpvlp dgaeghievn ldemgksydd
     2401 gfmskmaklp kkmfshgled eiavqepsit qhdihvdipk lkdeleirat lpekpqvdfd
     2461 vaipgkqvsl dlpegveski evsapdvgkp tdggfmskvv qlpkkmfphg skgtvpleea
     2521 peavgdihak vpeahlkvgl kglekpdfeg diavpgkkga vslpegtegk ievstpetgk
     2581 psdkgfmskm aklpkkmfph gskgsvsvee vpevdrsvkv kvpqgkipea hlkvdikdpe
     2641 khkdvdials eekvdidlpe gidknievtm pevskpsegg fiskmakfpk kmfphgakgs
     2701 vsveeapgid vdvkmpetri smdvkglekp kgevdislpk ekvdidvpkg fdgklemsap
     2761 evgkpseggf mskmakfpkk mfphgakgsv sveeapgidv dvkvpeahis mdvkglekpr
     2821 gevdislpke kvdidvpkgf dgklemsape vgkpseggfm skmakfpkkm fphgakgsls
     2881 veetpgidvd vkmpeadlsm dvkglekpka eldiampkgk ididmpegvd gkleasapdi
     2941 gkpsdggfis kmtkfpkkmf phgakgtvsv eapelgdvqv nmpeahlgvd ikgpekpkge
     3001 ldvtlpkgkv didlpegieg kveisapeig kpdggfmskm aklpkkmfph gakggvsveg
     3061 spelgdvevk mpeahlgvdi kgpekpkgel dvtlpkgkvd idlpegiegk veisapeigk
     3121 pdggfmskma klpkkmfphg akggvsvegs pelgdvevkm peahlgvdik gpekpkgeld
     3181 vtlpkgkvdi dlpegiegkv eisapeigkp dggfmskmak lpkkmfphga kggvsvegsp
     3241 elgdvevkmp eahlgvdikg pekpkgeldv tlpkgkvdid lpegiegkve isapeigkpd
     3301 ggfmskmakl pkkmfshgak ggvsvegspe lgdvqvkmpe ahlgvdikgp ekpkgeldvt
     3361 lpkgkvdidl pegiegkvei sspeigkpdg gfmskmaklp kkmfphgakg gvsvegapel
     3421 gdvevkmpea hlgvdikgpe kpkgeldvtl pkgkvdidlp egiegkveis apeigkpdgg
     3481 fmskmaklpk kmfphgakgg vsvegapelg dvevkmpeah lgvdikgpek pkgeldvtlp
     3541 kgkvdidlpe gfegkveisa peigkpdggf mskmaklpkk mfphgtkggv svegapelgd
     3601 vevkmpeahl gvdikgpekp kgeldvtlpk gkvdidlpeg iegkveissp eigkpdggfm
     3661 skmaklpkkm fphgakggvs vegapelgdv evkmpeahlg vdikgpekpk geldvtlpkg
     3721 kvdidlpegi egkveisape igkpdggfms kmaklpkkmf phgakggvsv egspelgdve
     3781 vkmpeahlgv dikgpekpkg eldvtlpkgk vdidlpegie gkleisapei gkpdggfmsk
     3841 makfpkkmfp hgakggvsve gapelgdvqv kmpeahlgvd ikgpekpkge ldvtlpkgkv
     3901 didlpegieg kveisapeig kpdggfmskm akfpkkmfph gakggvsveg apelgdvqvk
     3961 mpeahlgvdi egpekpkgel dvtlpkgkvd idlpegiegk veisapeigk pdggfmskma
     4021 kfpkkmfphg tkggvsvega pelgdvevkm peahlgvdik gpekpkgeld vtlpkgkvdi
     4081 dlpegiegkv eisapeigkp dggfmskmak lpqkmfphgt kggvsvegap elgdiqlkmp
     4141 eahlgvdikg pekpkgelda mlpkgkvdid lpegiegkve isapeigkpd ggfmskmakl
     4201 pkkmfshgak gsisvegspe fgdvevkmpe ahlggdikgp ekpkgeldva lpkgkvdidi
     4261 pegiegkvei sapeigkpsd ggfvskmakl pkkmfphgtk gsvsiegape vgdvqvkvpe
     4321 aqlkmdikgt gkpefegeit wtgkkvavel pegiegtvem stpemgrpsd ggfmskvakl
     4381 pkkvfshgsk ggvpfdeetg vagdievkms eaalkvdvky pekpesdfsa lekkididvp
     4441 egfkgkveis tpelgkpsdg gflskmsklp kkvlphgskg eisagevsev sgevpvelpe
     4501 arlkvgvkgp erpdsdvdis vsdkkvtldv tkgfegkvev stpdlgkpsd kgfiskvtkf
     4561 pkkmfphgsk ggvsvgevse vtgdvhvelp dahlkvgvkg perpdsdvdv smavkkitvd
     4621 vpegfegkle vstpelarsp dgslvskaak lpkklvpsge nslssveeps lsahvdvpgs
     4681 dttfkvdakl pampvtdvdi slsakessad ipvgvagkve igtpdagkps dkgfmskmak
     4741 ipkkmfhhgp kssgstdehs tvaetvddka lgfevqlqkd vkiptkpead vsvsfhekev
     4801 tvqlpegreg tvelstpglv gehvpdkssq gadvllpgke lptvegqeii ekpggvievi
     4861 evtahelskp lssgllskvs kipkkifprg skskssdeds pspgkrelke tpggivevve
     4921 vtkhdvaihp esthvkqvgt evllepatse tkpsgaeglp pthitreike vpggivevvr
     4981 vtkhivgklp edkgigepsv rttdaslpwe aelakdpsav ikrevketpe ghvevvqvir
     5041 reasdvpfgs dfpgdlesdg tvsrriettv vdgkptvirr lvtttsdgir qvtehieqga
     5101 edagsaassl fakmgefakg lssddpsavt trevkqlpgg avevvsvtkr sssspeteit
     5161 sqeqlqssts gsmhfaesav gkpmeaktti irreisgpet ivrtttsvvt satapgsskp
     5221 elsadvldkm fgamekprvd drlrdvltae ggyvakrevt peeaellaql fpeqstgvav
     5281 yppaglvpea aiipeyrcmt taegahhdpe skekvllklg ralsqddlda psptqgeaam
     5341 tvaglgeavd tsdqveepav eklelarres krysqefeqd eplpkamgfk tkpseetvvs
     5401 stqvkgvhdn gtvkhtvttv tkttiiqeve epagaprpge setwasdldt sqvsvsslpe
     5461 derteptlss sattatadae alsgplartq veekewtetd pdsgevrhii sktthttvts
     5521 kkpsegaprg dttsttvshs skdghhgstp sapsecdeae sltffqkkef feklseqspp
     5581 ketssarlgs tpsldtaltr pavtlkprpr sesltsqgei ieesivfaer lrlfqngaqa
     5641 splpsasipq vdlvdpapkm eqklqgmaei gdslqqvpsa vleglqstee ksdtrllvht
     5701 sdadkeafdv rsvqvaedfg dkqfshmrvr mpapeetvsk tsicssdlpg dkpsplrday
     5761 kgslsasrqe ssqagkpplp salggdsslc lvheattdlt lskagppnqt nkdittfdrq
     5821 pvreqsptlr ssqsqmlasg lqmgivagsg ilqegikasp qsailetfss satemtgles
     5881 peirglpsee vhagsaeeak gidagrigav laaapvailp vrdiisgkql emldiaqvsd
     5941 epnifegcve lpsqaesqtc pkektimkte yadiqhklss afgeslapve sksgvtqgvp
     6001 kevlealdrs sqeliaskld edserdtnil satqgsiphd vfpdhiestl dnsaavpmrg
     6061 aystpsstav cltperleiq atrevaeerq ekpevlvale ayrkstlrrs gedsydrnle
     6121 efitvleeye plpagqlsdw kraspafeps ichnseitsk lppledkkel etvgsildps
     6181 envsellgnh aqsqmlcedt glltdelgrt aghyvistae sdrgeclptl seqapgkere
     6241 eclnydsalg vrstsgless lleesheers ldlarsedeq pslvedsmnv ptessvidyq
     6301 vvghsgvvkd aqtqpkdsdf dlheisrtgd ktdlplscgd dgkksaefsk tvsgiaadmv
     6361 ikdtdsehls qdmlhptast vlcegrsdvp yssdedqhke lsrykevelv ipflrgtsss
     6421 ledevedggs fssgdlqaek iaeflttdpd tlnkvvhfpq ifqdydtsah qepfvptete
     6481 mnvekhvgfv ldemaacstp cvplksdrqt eieeasrive svvhiasslv eslhevnhet
     6541 aikedicdsa layaagsple rsevsetlsq agppstiekd qrespfgvke myqvlkesrg
     6601 sedriqysvt sfgdplfade gdedqerrrr gsptaesldd qaqiekcshe vmmqlldgfs
     6661 qsdvelaevr tedeapdied dvpspeagsh eeksvpssqd nsgdlqpqls teveigsqas
     6721 gapaplseld yelcpdadql rtsgdeavat rvppleperd eavaesshap saaadpaasa
     6781 grnaggrers plleasppee taycpeqdis paqeypeylt pdmqvrvgvs pteaqpakek
     6841 pvrysessve sssevgspcp psqggtamaf envafagedv idatsspiee ssspagsrsp
     6901 yevvrdahae eaaqtalvea ekslrhavqe evrarpqslv teesseqqeh qiqpsifnea
     6961 fdhspeesap ekkqlsegll gvglltvsdt tartpesrrg dsdedeeewa dhvmskirgr
     7021 pipptppasp ravqsavevq gepweapvve qervnvesar dmsaerdagv dvmgdrsatq
     7081 pqlqefrrta dgkrpqlehi pseisdedlv ereqspthit atcrgdrkds ssdrnsspds
     7141 dgpkasgshr rsasgvgaia leavhsqdsg gnekhshesr dkgaqeddki teepvdtmfv
     7201 ieeeapadsn rsesfcddad eraartaggl spslesppqk kdlddgsssg kpdaaspvvl
     7261 rskskrtdki sdkrssrdld tghsgsetdh shyysfelts dsgktpgasr ptssefdvni
     7321 lsgqasseye tcatsqdgev sgsyataaas shdtsfatar sslsgssrss aysvdsessg
     7381 hlgsmevsea setivasahe eldsdieser sksvlrepyd geipesvirg gvehpflpsw
     7441 qpalkdsspt lhgsagarpp taegvfspfq stvpsepgrs cvgwpsssls taesvrspsk
     7501 pevlngvsap legqrrlrhn ddfmasshee eqlsvsessa tqsehtwqts vetmvdavea
     7561 qrfscdserh hrqstmqnsv slpfdeavva edshreaddd ratppfqevn gpveveyvpe
     7621 yddltartlr pvrqqnapdg qteaqgdval leasagdast sreqheeens telreegfya
     7681 etfqgdlsse evesaaneal lldksacsfy eychakdeye vcntrpaves aslkeaferp
     7741 atpepvdeid dqedegnlaq gspnlhgkdy eafghasaee svpcsdkthi aqelsendqy
     7801 kvfsdsgnqs slhdetrsks vsssleramt ddvrlytskc edyilqmhqt ykygvglpkt
     7861 sdggylpeeg mdvenvklle srlqegnggq qdfpydlsyq qeyggdgrve hgdlythqev
     7921 eeegydders rtrgrrrlsp isdergstpd ydtlagrktf gkgsekddas tsslmeferl
     7981 eaemasgtks ssvgssdsls grqhqgrsga gseqdsvsvn slteferlek eiveaegvda
     8041 rlrgevaakl deideghesq asdpghetav ydesgatlll casadvneep dgttddaqlg
     8101 dieaaqgqev awsrterhfl ttrtesghsv esvdrqssas tatqfdadsl rereiddses
     8161 nfgpdtpgwm qkecvdldsl hdslhdsehh dslqevpskd kdslqlreps adadslqedh
     8221 nelqdslyet rdidwdsfra detsglddts rswhqfpsqi rttelsptls rpassplvvp
     8281 vvveridtlt wvdqdpakac vsdgeslgsr qqehprndal vsstdsleps ssanthatyq
     8341 cdtdsmmssl nsaeestmvt stdtlehetl clasqgksls legglfsggd vteeqntrtp
     8401 leggvsvddm eerfrecllr saasptneeq veerqtmdek gnlivtrtvk rhvtseprlh
     8461 tqtfmgpdae qrsrefvasf aamepsaerd eyegfdaagn tvrvtqhvvv rptiktvtft
     8521 gpdarlqmre ymrslaggqh ssaentptsd sgvgacssla sppplmpdpp aaapssacpg
     8581 lqrlsggdqw meaesalddc sppvtatrvi ttrttrtmgp dgreqlvttt teesggeepe
     8641 arlrrsmqgv ldsfmaeppt pphldeee
//
LOCUS       XP_077503813            8667 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X21 [Amblyomma
            americanum].
ACCESSION   XP_077503813
VERSION     XP_077503813.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647687.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8667
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..8667
                     /product="uncharacterized protein LOC144114148 isoform
                     X21"
                     /calculated_mol_wt=930304
     Region          24..55
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          38..355
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(57,59,63..64,67..69,71..72,76,79,88,90,92,96..97,
                     100..102,104..105,109,112,121,123,125,129..130,133..135,
                     137..138,142,145)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          57..88
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          90..121
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          123..148
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          156..181
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          189..216
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(218,220,224..225,228..230,232..233,237,240,249,251,
                     253,257..258,261..263,265..266,270,273,282,284,286,
                     290..291,294..296,298..299,303,306)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          218..249
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          251..282
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          284..314
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          298..586
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          317..381
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          383..414
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(416,418,422..423,426..428,430..431,435,438,447,449,
                     451,455..456,459..461,463..464,468,471,480,482,484,
                     488..489,492..494,496..497,501,504)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          416..447
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          449..480
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          464..742
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          482..511
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          515..540
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          548..579
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          581..614
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          615..645
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          647..677
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(680,682,686..687,690..692,694..695,699,702,711,713,
                     715,719..720,723..725,727..728,732,735,744,746,748,
                     752..753,756..758,760..761,765,768)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          680..711
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          713..744
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          718..799
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          746..776
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          930..1033
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1256..1388
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1420..1503
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <1816..2067
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          <4430..4690
                     /region_name="YbbR"
                     /note="Cyclic di-AMP synthase regulator CdaR, YbbR domain
                     [Signal transduction mechanisms]; COG4856"
                     /db_xref="CDD:443884"
     Region          <7095..7408
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..8667
                     /gene="LOC144114148"
                     /coded_by="XM_077647687.1:278..26281"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 maitahdapp gspngqipds aqtdgnasfl raaragnlek vldylkgsid intsnangln
       61 alhlaakegh vnvvsellkr ganvnattkk gnsalhiasl agqeevvkll vqkqanvnvq
      121 sqngftplym aaqenhdgvv rfllanganq slatedgftp lavalqqghd kvvavllend
      181 argkvrlpal hiaskkddck aaalllhseh npdvtsksgf tplhiaahyg nsniasllld
      241 kgadvnfpak hqitplhvaa kwgksnmvkl llekgakida strdgltplh caarsghdqv
      301 veqlleknap itaktkngla plhmasqgdh vdsarillyh kapvddvtvd yltalhvaah
      361 cghvgvakll ldrradpnar alngftplhi ackknrikvv elllkhgasi eattesgltp
      421 lhvasfmgcm niviyliqhg anpdiptvrg etplhlaara nqtdiirill rngahvdaka
      481 relqtalhia srlgnadmvg lllqhgaavd aptkdaytpl hvaaregqde vaallldhga
      541 alaaptkkgf tplhlaakyg nlkvaqlllq kdapvdaqgk ngvtplhvaa hydhvnvall
      601 llekgasphs aarngytplh vaarkdqmdi asslleygak pgaesragft plhlaaqegh
      661 adlaallveh gadcdakakn gltpmhlcaq edrvevatil akhgasldpt tkagytplhv
      721 achfgqtnmi rfllrqganv nattshgytp lhqaaqqght liinlllehr aapnavtnqg
      781 qtalaiaqrl gyisvvetlk vvtetivttt tttvteekyr vvapetmhet fmsdsedeaa
      841 ednmlgdqsf ryltademks lgddslpidv trderitesi hitrepghpa pltqeeerls
      901 ptqahtteai fvgnyapdnv dlsrtpihag flvsflvdar ggamrgcrhs gvrviipprk
      961 aqmpmritcr ylrkeklphp ppllegeaca srilevgpag akflgpvile vphfaslrgk
     1021 ereisilrsd ngetwkehtl easeeavqev lnesfegeel saledlqtnr ivrilttdfp
     1081 qyfavvsrtr qevhavgpeg gllsstvvpq vqaifpegal tkkirvglqa qpipaelvtk
     1141 llghrvavsp ivtveprrrk fhkpitltip vpqaatkgmi nqyagdaptl rllcsitggt
     1201 nkaqwedvtg stpltfvndc vsftttvsar fwlmdcrqvs eatkfately aeamhvpfma
     1261 kfvvfakrqd pmeaqlrvfc mtddkedktl esqehfteva ksrdvevlek ksqylefggn
     1321 lvpvtksgeq lqlqfqafre nrlpfavrvk dphqeplgrl afmrqpraar ateppqaplc
     1381 nlnialpeye asqnlselvt lekkygfvee tglakpelih radlrlsdia relgsdwpal
     1441 aaqldvpeqd vasirtecps dlaqqallml rlwmkraggr atgnnlekgl rminredivn
     1501 kcmfnvelvt ddvekavakv hldqsgfdtf reelgtpkdt slkrdasldv sydeqdlmka
     1561 esaeetsset gsvhekhtgs eakgtskaip gvrkgspisa aigrivrgss kekaaprmav
     1621 eetfeeepvl kidsaevtqq aapeagptap prdskkkskk ekspsplgpd yslprmrdyh
     1681 tperdftstt dtlivssike dvdaskelat dgkvkkekkk ekkekspspp gpdytlprlr
     1741 dtytppsdvt aagyaastlp gqgitleqgi vkeplerksk erslspgvvk tgengslgad
     1801 psverqvkql sqgpedtiye kvervvketp gkivemvhik rqrsskspeg rgspkdknkg
     1861 lpssevvapd isgesrsvps etigaekkqk khketsptkq gkkeakevse avdkdaelsa
     1921 sdgklpdsgf lskmaklpkk mfprsgkasa sdeepsslse sspvakkkag klpksatlpk
     1981 akpsktplye iksvdvevdt skltskgvkg vpakdgegle tsrdkepdsr ffskmaripk
     2041 kmfprsskts ssedepssas enlpsskqra kkekeisskk kekvvkvkpd skklstsfev
     2101 evdrdtlpga gvdinakkpg lqeslesesr kktdsmqisf pqsvkavgsk gepmeysldi
     2161 egmkslqkep qlavvgefpg kpspdielsl tsketapefs dkarghieig tpehgksskg
     2221 gfmtkmaklp rkmfphgfkg dvsveepsve aesvhvsvpe aqvklgtkdl kkkgpdmpam
     2281 eltaslpvga egsvemsapd lpvsadggli skvtqfpkmf shdsqdgvti erppvvgegi
     2341 dvesslpdvq lkvkgkafek petkfdvtlp erkvtpvlpd gaeghievnl demgksyddg
     2401 fmskmaklpk kmfshglede iavqepsitq hdihvdipkl kdeleiratl pekpqvdfdv
     2461 aipgkqvsld lpegveskie vsapdvgkpt dggfmskvvq lpkkmfphgs kgtvpleeap
     2521 eavgdihakv peahlkvglk glekpdfegd iavpgkkgav slpegtegki evstpetgkp
     2581 sdkgfmskma klpkkmfphg skgsvsveev pevdrsvkvk vpqgkipeah lkvdikdpek
     2641 hkdvdialse ekvdidlpeg idknievtmp evskpseggf iskmakfpkk mfphgakgsv
     2701 sveeapgidv dvkmpetris mdvkglekpk gevdislpke kvdidvpkgf dgklemsape
     2761 vgkpseggfm skmakfpkkm fphgakgsvs veeapgidvd vkvpeahism dvkglekprg
     2821 evdislpkek vdidvpkgfd gklemsapev gkpseggfms kmakfpkkmf phgakgslsv
     2881 eetpgidvdv kmpeadlsmd vkglekpkae ldiampkgki didmpegvdg kleasapdig
     2941 kpsdggfisk mtkfpkkmfp hgakgtvsve apelgdvqvn mpeahlgvdi kgpekpkgel
     3001 dvtlpkgkvd idlpegiegk veisapeigk pdggfmskma klpkkmfphg akggvsvegs
     3061 pelgdvevkm peahlgvdik gpekpkgeld vtlpkgkvdi dlpegiegkv eisapeigkp
     3121 dggfmskmak lpkkmfphga kggvsvegsp elgdvevkmp eahlgvdikg pekpkgeldv
     3181 tlpkgkvdid lpegiegkve isapeigkpd ggfmskmakl pkkmfphgak ggvsvegspe
     3241 lgdvevkmpe ahlgvdikgp ekpkgeldvt lpkgkvdidl pegiegkvei sapeigkpdg
     3301 gfmskmaklp kkmfshgakg gvsvegspel gdvqvkmpea hlgvdikgpe kpkgeldvtl
     3361 pkgkvdidlp egiegkveis speigkpdgg fmskmaklpk kmfphgakgg vsvegapelg
     3421 dvevkmpeah lgvdikgpek pkgeldvtlp kgkvdidlpe giegkveisa peigkpdggf
     3481 mskmaklpkk mfphgakggv svegapelgd vevkmpeahl gvdikgpekp kgeldvtlpk
     3541 gkvdidlpeg fegkveisap eigkpdggfm skmaklpkkm fphgtkggvs vegapelgdv
     3601 evkmpeahlg vdikgpekpk geldvtlpkg kvdidlpegi egkveisspe igkpdggfms
     3661 kmaklpkkmf phgakggvsv egapelgdve vkmpeahlgv dikgpekpkg eldvtlpkgk
     3721 vdidlpegie gkveisapei gkpdggfmsk maklpkkmfp hgakggvsve gspelgdvev
     3781 kmpeahlgvd ikgpekpkge ldvtlpkgkv didlpegieg kleisapeig kpdggfmskm
     3841 akfpkkmfph gakggvsveg apelgdvqvk mpeahlgvdi kgpekpkgel dvtlpkgkvd
     3901 idlpegiegk veisapeigk pdggfmskma kfpkkmfphg akggvsvega pelgdvqvkm
     3961 peahlgvdie gpekpkgeld vtlpkgkvdi dlpegiegkv eisapeigkp dggfmskmak
     4021 fpkkmfphgt kggvsvegap elgdvevkmp eahlgvdikg pekpkgeldv tlpkgkvdid
     4081 lpegiegkve isapeigkpd ggfmskmakl pqkmfphgtk ggvsvegape lgdiqlkmpe
     4141 ahlgvdikgp ekpkgeldam lpkgkvdidl pegiegkvei sapeigkpdg gfmskmaklp
     4201 kkmfshgakg sisvegspef gdvevkmpea hlggdikgpe kpkgeldval pkgkvdidip
     4261 egiegkveis apeigkpsdg gfvskmaklp kkmfphgtkg svsiegapev gdvqvkvpea
     4321 qlkmdikgtg kpefegeitw tgkkvavelp egiegtvems tpemgrpsdg gfmskvaklp
     4381 kkvfshgskg gvpfdeetgv agdievkmse aalkvdvkyp ekpesdfsal ekkididvpe
     4441 gfkgkveist pelgkpsdgg flskmsklpk kvlphgskge isagevsevs gevpvelpea
     4501 rlkvgvkgpe rpdsdvdisv sdkkvtldvt kgfegkvevs tpdlgkpsdk gfiskvtkfp
     4561 kkmfphgskg gvsvgevsev tgdvhvelpd ahlkvgvkgp erpdsdvdvs mavkkitvdv
     4621 pegfegklev stpelarspd gslvskaakl pkklvpsgen slssveepsl sahvdvpgsd
     4681 ttfkvdaklp ampvtdvdis lsakessadi pvgvagkvei gtpdagkpsd kgfmskmaki
     4741 pkkmfhhgpk ssgstdehst vaetvddkal gfevqlqkdv kiptkpeadv svsfhekevt
     4801 vqlpegregt velstpglvg ehvpdkssqg advllpgkel ptvegqeiie kpggvievie
     4861 vtahelskpl ssgllskvsk ipkkifprgs kskssdedsp spgkrelket pggivevvev
     4921 tkhdvaihpe sthvkqvgte vllepatset kpsgaeglpp thitreikev pggivevvrv
     4981 tkhivgklpe dkgigepsvr ttdaslpwea elakdpsavi krevketpeg hvevvqvirr
     5041 easdvpfgsd fpgdlesdgt vsrriettvv dgkptvirrl vtttsdgirq vtehieqgae
     5101 dagsaasslf akmgefakgl ssddpsavtt revkqlpgga vevvsvtkrs ssspeteits
     5161 qeqlqsstsg smhfaesavg kpmeakttii rreisgpeti vrtttsvvts atapgsskpe
     5221 lsadvldkmf gamekprvdd rlrdvltaeg gyvakrevtp eeaellaqlf peqstgvavy
     5281 ppaglvpeaa iipeyrcmtt aegahhdpes kekvllklgr alsqddldap sptqgeaamt
     5341 vaglgeavdt sdqveepave klelarresk rysqefeqde plpkamgfkt kpseetvvss
     5401 tqvkgvhdng tvkhtvttvt kttiiqevee pagaprpges etwasdldts qvsvsslped
     5461 erteptlsss attatadaea lsgplartqv eekewtetdp dsgevrhiis ktthttvtsk
     5521 kpsegaprgd ttsttvshss kdghhgstps apsecdeaes ltffqkkeff eklseqsppk
     5581 etssarlgst psldtaltrp avtlkprprs esltsqgeii eesivfaerl rlfqngaqas
     5641 plpsasipqv dlvdpapkme qklqgmaeig dslqqvpsav leglqsteek sdtrllvhts
     5701 dadkeafdvr svqvaedfgd kqfshmrvrm papeetvskt sicssdlpgd kpsplrdayk
     5761 gslsasrqes sqagkpplps alggdsslcl vheattdltl skagppnqtn kdittfdrqp
     5821 vreqsptlrs sqsqmlasgl qmgivagsgi lqegikaspq sailetfsss atemtglesp
     5881 eirglpseev hagsaeeakg idagrigavl aaapvailpv rdiisgkqle mldiaqvsde
     5941 pnifegcvel psqaesqtcp kektimktey adiqhklssa fgeslapves ksgvtqgvpk
     6001 evlealdrss qeliasklde dserdtnils atqgsiphdv fpdhiestld nsaavpmrga
     6061 ystpsstavc ltperleiqa trevaeerqe kpevlvalea yrkstlrrsg edsydrnlee
     6121 fitvleeyep lpagqlsdwk raspafepsi chnseitskl ppledkkele tvgsildpse
     6181 nvsellgnha qsqmlcedtg lltdelgrta ghyvistaes drgeclptls eqapgkeree
     6241 clnydsalgv rstsglessl leesheersl dlarsedeqp slvedsmnvp tessvidyqv
     6301 vghsgvvkda qtqpkdsdfd lheisrtgdk tdlplscgdd gkksaefskt vsgiaadmvi
     6361 kdtdsehlsq dmlhptastv lcegrsdvpy ssdedqhkel srykevelvi pflrgtsssl
     6421 edevedggsf ssgdlqaeki aeflttdpdt lnkvvhfpqi fqdydtsahq epfvptetem
     6481 nvekhvgfvl demaacstpc vplksdrqte ieeasrives vvhiasslve slhevnheta
     6541 ikedicdsal ayaagspler sevsetlsqa gppstiekdq respfgvkem yqvlkesrgs
     6601 edriqysvts fgdplfadeg dedqerrrrg sptaeslddq aqiekcshev mmqlldgfsq
     6661 sdvelaevrt edeapdiedd vpspeagshe eksvpssqdn sgdlqpqlst eveigsqasg
     6721 apaplseldy elcpdadqlr tsgdeavatr vppleperde avaesshaps aaadpaasag
     6781 rnaggrersp lleasppeet aycpeqdisp aqeypeyltp dmqvrvgvsp teaqpakekp
     6841 vrysessves ssevgspcpp sqggtamafe nvafagedvi datsspiees sspagsrspy
     6901 evvrdahaee aaqtalveae kslrhavqee vrarpqslvt eesseqqehq iqpsifneaf
     6961 dhspeesape kkqlsegllg vglltvsdtt artpesrrgd sdedeeewad hvmskirgrp
     7021 ipptppaspr avqsavevqg epweapvveq ervnvesard msaerdagvd vmgdrsatqp
     7081 qlqefrrtad gkrpqlehip seisdedlve reqspthita tcrgdrkdss sdrnsspdsd
     7141 gpkasgshrr sasgvgaial eavhsqdsgg nekhshesrd kgaqeddkit eepvdtmfvi
     7201 eeeapadsnr sesfcddade raartaggls pslesppqkk dlddgsssgk pdaaspvvlr
     7261 skskrtdkis dkrssrdldt ghsgsetdhs hyysfeltsd sgktpgasrp tssefdvnil
     7321 sgqasseyet catsqdgevs gsyataaass hdtsfatars slsgssrssa ysvdsessgh
     7381 lgsmevseas etivasahee ldsdiesers ksvlrepydg eipesvirgg vehpflpswq
     7441 palkdssptl hgsagarppt aegvfspfqs tvpsepgrsc vgwpssslst aesvrspskp
     7501 evlngvsapl egqrrlrhnd dfmassheee qlsvsessat qsehtwqtsv etmvdaveaq
     7561 rfscdserhh rqstmqnsvs lpfdeavvae dshreadddr atppfqevng pveveyvpey
     7621 ddltartlrp vrqqnapdgq teaqgdvall easagdasts reqheeenst elreegfyae
     7681 tfqgdlssee vesaaneall ldksacsfye ychakdeyev cntrpavesa slkeaferpa
     7741 tpepvdeidd qedegnlaqg spnlhgkdye afghasaees vpcsdkthia qelsendqyk
     7801 vfsdsgnqss lhdetrsksv sssleramtd dvrlytskce dyilqmhqty kygvglpkts
     7861 dggylpeegm dvenvklles rlqegnggqq dfpydlsyqq eyggdgrveh gdlythqeve
     7921 eegyddersr trgrrrlspi sdergstpdy dtlagrktfg kgsekddast sslmeferle
     7981 aemasgtkss svgssdslsg rqhqgrsgag seqdsvsvns lteferleke iveaegvdar
     8041 lrgevaakld eideghesqa sdpghetavy desgatlllc asadvneepd gttddaqlgd
     8101 ieaaqgqeva wsrterhflt trtesghsve svdrqssast atqfdadslr ereiddsesn
     8161 fgpdtpgwmq kecvdldslh dslhdsehhd slqevpskdk dslqlrepsa dadslqedhn
     8221 elqdslyetr didwdsfrad etsglddtsr swhqfpsqir ttelsptlsr passplvvpv
     8281 vveridtltw vdqdpakacv sdgeslgsrq qehprndalv sstdslepss santhatyqc
     8341 dtdsmmssln saeestmvts tdtlehetlc lasqgkslsl egglfsggdv teeqntrtpl
     8401 eggvsvddme erfrecllrs aasptneeqv eerqtmdekg nlivtrtvkr hvtseprlht
     8461 qtfmgpdaeq rsrefvasfa amepsaerde yegfdaagnt vrvtqhvvvr ptiktvtftg
     8521 pdarlqmrey mrslaggqhs saentptsds gvgacsslas ppplmpdppa aapssacpgl
     8581 qrlsggdqwm eaesalddcs ppvtatrvit trttrtmgpd greqlvtttt eesggeepea
     8641 rlrrsmqgvl dsfmaepptp phldeee
//
LOCUS       XP_077503814            8656 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X22 [Amblyomma
            americanum].
ACCESSION   XP_077503814
VERSION     XP_077503814.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647688.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8656
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..8656
                     /product="uncharacterized protein LOC144114148 isoform
                     X22"
                     /calculated_mol_wt=929331
     Region          12..43
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          26..343
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(45,47,51..52,55..57,59..60,64,67,76,78,80,84..85,
                     88..90,92..93,97,100,109,111,113,117..118,121..123,
                     125..126,130,133)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          45..76
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          78..109
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          111..136
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          144..169
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          177..204
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(206,208,212..213,216..218,220..221,225,228,237,239,
                     241,245..246,249..251,253..254,258,261,270,272,274,
                     278..279,282..284,286..287,291,294)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          206..237
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          239..270
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          272..302
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          286..574
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          305..369
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          371..402
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(404,406,410..411,414..416,418..419,423,426,435,437,
                     439,443..444,447..449,451..452,456,459,468,470,472,
                     476..477,480..482,484..485,489,492)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          404..435
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          437..468
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          452..730
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          470..499
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          503..528
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          536..567
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          569..602
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          603..633
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          635..665
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(668,670,674..675,678..680,682..683,687,690,699,701,
                     703,707..708,711..713,715..716,720,723,732,734,736,
                     740..741,744..746,748..749,753,756)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          668..699
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          701..732
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          706..787
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          734..764
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          918..1021
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1244..1376
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1408..1491
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <1805..2056
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          <4419..4679
                     /region_name="YbbR"
                     /note="Cyclic di-AMP synthase regulator CdaR, YbbR domain
                     [Signal transduction mechanisms]; COG4856"
                     /db_xref="CDD:443884"
     Region          <7084..7397
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..8656
                     /gene="LOC144114148"
                     /coded_by="XM_077647688.1:360..26330"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 mgaaqrklak tdgnasflra aragnlekvl dylkgsidin tsnanglnal hlaakeghvn
       61 vvsellkrga nvnattkkgn salhiaslag qeevvkllvq kqanvnvqsq ngftplymaa
      121 qenhdgvvrf llanganqsl atedgftpla valqqghdkv vavllendar gkvrlpalhi
      181 askkddckaa alllhsehnp dvtsksgftp lhiaahygns niasllldkg advnfpakhq
      241 itplhvaakw gksnmvklll ekgakidast rdgltplhca arsghdqvve qlleknapit
      301 aktknglapl hmasqgdhvd sarillyhka pvddvtvdyl talhvaahcg hvgvakllld
      361 rradpnaral ngftplhiac kknrikvvel llkhgasiea ttesgltplh vasfmgcmni
      421 viyliqhgan pdiptvrget plhlaaranq tdiirillrn gahvdakare lqtalhiasr
      481 lgnadmvgll lqhgaavdap tkdaytplhv aaregqdeva allldhgaal aaptkkgftp
      541 lhlaakygnl kvaqlllqkd apvdaqgkng vtplhvaahy dhvnvallll ekgasphsaa
      601 rngytplhva arkdqmdias slleygakpg aesragftpl hlaaqeghad laallvehga
      661 dcdakakngl tpmhlcaqed rvevatilak hgasldpttk agytplhvac hfgqtnmirf
      721 llrqganvna ttshgytplh qaaqqghtli inlllehraa pnavtnqgqt alaiaqrlgy
      781 isvvetlkvv tetivttttt tvteekyrvv apetmhetfm sdsedeaaed nmlgdqsfry
      841 ltademkslg ddslpidvtr deritesihi trepghpapl tqeeerlspt qahtteaifv
      901 gnyapdnvdl srtpihagfl vsflvdargg amrgcrhsgv rviipprkaq mpmritcryl
      961 rkeklphppp llegeacasr ilevgpagak flgpvilevp hfaslrgker eisilrsdng
     1021 etwkehtlea seeavqevln esfegeelsa ledlqtnriv rilttdfpqy favvsrtrqe
     1081 vhavgpeggl lsstvvpqvq aifpegaltk kirvglqaqp ipaelvtkll ghrvavspiv
     1141 tveprrrkfh kpitltipvp qaatkgminq yagdaptlrl lcsitggtnk aqwedvtgst
     1201 pltfvndcvs ftttvsarfw lmdcrqvsea tkfatelyae amhvpfmakf vvfakrqdpm
     1261 eaqlrvfcmt ddkedktles qehftevaks rdvevlekks qylefggnlv pvtksgeqlq
     1321 lqfqafrenr lpfavrvkdp hqeplgrlaf mrqpraarat eppqaplcnl nialpeyeas
     1381 qnlselvtle kkygfveetg lakpelihra dlrlsdiare lgsdwpalaa qldvpeqdva
     1441 sirtecpsdl aqqallmlrl wmkraggrat gnnlekglrm inredivnkc mfnvelvtdd
     1501 vekavakvhl dqsgfdtfre elgtpkdtsl krdasldvsy deqdlmkeae saeetssetg
     1561 svhekhtgse akgtskaipg vrkgspisaa igrivrgssk ekaaprmave etfeeepvlk
     1621 idsaevtqqa apeagptapp rdskkkskke kspsplgpdy slprmrdyht perdftsttd
     1681 tlivssiked vdaskelatd gkvkkekkke kkekspsppg pdytlprlrd tytppsdvta
     1741 agyaastlpg qgitleqgiv keplerkske rslspgvvkt gengslgadp sverqvkqls
     1801 qgpedtiyek vervvketpg kivemvhikr qrsskspegr gspkdknkgl pssevvapdi
     1861 sgesrsvpse tigaekkqkk hketsptkqg kkeakevsea vdkdaelsas dgklpdsgfl
     1921 skmaklpkkm fprsgkasas deepsslses spvakkkagk lpksatlpka kpsktplyei
     1981 ksvdvevdts kltskgvkgv pakdgeglet srdkepdsrf fskmaripkk mfprssktss
     2041 sedepssase nlpsskqrak kekeisskkk ekvvkvkpds kklstsfeve vdrdtlpgag
     2101 vdinakkpgl qeslesesrk ktdsmqisfp qsvkavgskg epmeysldie gmkslqkepq
     2161 lavvgefpgk pspdielslt sketapefsd karghieigt pehgksskgg fmtkmaklpr
     2221 kmfphgfkgd vsveepsvea esvhvsvpea qvklgtkdlk kkgpdmpame ltaslpvgae
     2281 gsvemsapdl pvsadgglis kvtqfpkmfs hdsqdgvtie rppvvgegid vesslpdvql
     2341 kvkgkafekp etkfdvtlpe rkvtpvlpdg aeghievnld emgksyddgf mskmaklpkk
     2401 mfshgledei avqepsitqh dihvdipklk deleiratlp ekpqvdfdva ipgkqvsldl
     2461 pegveskiev sapdvgkptd ggfmskvvql pkkmfphgsk gtvpleeape avgdihakvp
     2521 eahlkvglkg lekpdfegdi avpgkkgavs lpegtegkie vstpetgkps dkgfmskmak
     2581 lpkkmfphgs kgsvsveevp evdrsvkvkv pqgkipeahl kvdikdpekh kdvdialsee
     2641 kvdidlpegi dknievtmpe vskpseggfi skmakfpkkm fphgakgsvs veeapgidvd
     2701 vkmpetrism dvkglekpkg evdislpkek vdidvpkgfd gklemsapev gkpseggfms
     2761 kmakfpkkmf phgakgsvsv eeapgidvdv kvpeahismd vkglekprge vdislpkekv
     2821 didvpkgfdg klemsapevg kpseggfmsk makfpkkmfp hgakgslsve etpgidvdvk
     2881 mpeadlsmdv kglekpkael diampkgkid idmpegvdgk leasapdigk psdggfiskm
     2941 tkfpkkmfph gakgtvsvea pelgdvqvnm peahlgvdik gpekpkgeld vtlpkgkvdi
     3001 dlpegiegkv eisapeigkp dggfmskmak lpkkmfphga kggvsvegsp elgdvevkmp
     3061 eahlgvdikg pekpkgeldv tlpkgkvdid lpegiegkve isapeigkpd ggfmskmakl
     3121 pkkmfphgak ggvsvegspe lgdvevkmpe ahlgvdikgp ekpkgeldvt lpkgkvdidl
     3181 pegiegkvei sapeigkpdg gfmskmaklp kkmfphgakg gvsvegspel gdvevkmpea
     3241 hlgvdikgpe kpkgeldvtl pkgkvdidlp egiegkveis apeigkpdgg fmskmaklpk
     3301 kmfshgakgg vsvegspelg dvqvkmpeah lgvdikgpek pkgeldvtlp kgkvdidlpe
     3361 giegkveiss peigkpdggf mskmaklpkk mfphgakggv svegapelgd vevkmpeahl
     3421 gvdikgpekp kgeldvtlpk gkvdidlpeg iegkveisap eigkpdggfm skmaklpkkm
     3481 fphgakggvs vegapelgdv evkmpeahlg vdikgpekpk geldvtlpkg kvdidlpegf
     3541 egkveisape igkpdggfms kmaklpkkmf phgtkggvsv egapelgdve vkmpeahlgv
     3601 dikgpekpkg eldvtlpkgk vdidlpegie gkveisspei gkpdggfmsk maklpkkmfp
     3661 hgakggvsve gapelgdvev kmpeahlgvd ikgpekpkge ldvtlpkgkv didlpegieg
     3721 kveisapeig kpdggfmskm aklpkkmfph gakggvsveg spelgdvevk mpeahlgvdi
     3781 kgpekpkgel dvtlpkgkvd idlpegiegk leisapeigk pdggfmskma kfpkkmfphg
     3841 akggvsvega pelgdvqvkm peahlgvdik gpekpkgeld vtlpkgkvdi dlpegiegkv
     3901 eisapeigkp dggfmskmak fpkkmfphga kggvsvegap elgdvqvkmp eahlgvdieg
     3961 pekpkgeldv tlpkgkvdid lpegiegkve isapeigkpd ggfmskmakf pkkmfphgtk
     4021 ggvsvegape lgdvevkmpe ahlgvdikgp ekpkgeldvt lpkgkvdidl pegiegkvei
     4081 sapeigkpdg gfmskmaklp qkmfphgtkg gvsvegapel gdiqlkmpea hlgvdikgpe
     4141 kpkgeldaml pkgkvdidlp egiegkveis apeigkpdgg fmskmaklpk kmfshgakgs
     4201 isvegspefg dvevkmpeah lggdikgpek pkgeldvalp kgkvdidipe giegkveisa
     4261 peigkpsdgg fvskmaklpk kmfphgtkgs vsiegapevg dvqvkvpeaq lkmdikgtgk
     4321 pefegeitwt gkkvavelpe giegtvemst pemgrpsdgg fmskvaklpk kvfshgskgg
     4381 vpfdeetgva gdievkmsea alkvdvkype kpesdfsale kkididvpeg fkgkveistp
     4441 elgkpsdggf lskmsklpkk vlphgskgei sagevsevsg evpvelpear lkvgvkgper
     4501 pdsdvdisvs dkkvtldvtk gfegkvevst pdlgkpsdkg fiskvtkfpk kmfphgskgg
     4561 vsvgevsevt gdvhvelpda hlkvgvkgpe rpdsdvdvsm avkkitvdvp egfegklevs
     4621 tpelarspdg slvskaaklp kklvpsgens lssveepsls ahvdvpgsdt tfkvdaklpa
     4681 mpvtdvdisl sakessadip vgvagkveig tpdagkpsdk gfmskmakip kkmfhhgpks
     4741 sgstdehstv aetvddkalg fevqlqkdvk iptkpeadvs vsfhekevtv qlpegregtv
     4801 elstpglvge hvpdkssqga dvllpgkelp tvegqeiiek pggvieviev tahelskpls
     4861 sgllskvski pkkifprgsk skssdedsps pgkrelketp ggivevvevt khdvaihpes
     4921 thvkqvgtev llepatsetk psgaeglppt hitreikevp ggivevvrvt khivgklped
     4981 kgigepsvrt tdaslpweae lakdpsavik revketpegh vevvqvirre asdvpfgsdf
     5041 pgdlesdgtv srriettvvd gkptvirrlv tttsdgirqv tehieqgaed agsaasslfa
     5101 kmgefakgls sddpsavttr evkqlpggav evvsvtkrss sspeteitsq eqlqsstsgs
     5161 mhfaesavgk pmeakttiir reisgpetiv rtttsvvtsa tapgsskpel sadvldkmfg
     5221 amekprvddr lrdvltaegg yvakrevtpe eaellaqlfp eqstgvavyp paglvpeaai
     5281 ipeyrcmtta egahhdpesk ekvllklgra lsqddldaps ptqgeaamtv aglgeavdts
     5341 dqveepavek lelarreskr ysqefeqdep lpkamgfktk pseetvvsst qvkgvhdngt
     5401 vkhtvttvtk ttiiqeveep agaprpgese twasdldtsq vsvsslpede rteptlsssa
     5461 ttatadaeal sgplartqve ekewtetdpd sgevrhiisk tthttvtskk psegaprgdt
     5521 tsttvshssk dghhgstpsa psecdeaesl tffqkkeffe klseqsppke tssarlgstp
     5581 sldtaltrpa vtlkprprse sltsqgeiie esivfaerlr lfqngaqasp lpsasipqvd
     5641 lvdpapkmeq klqgmaeigd slqqvpsavl eglqsteeks dtrllvhtsd adkeafdvrs
     5701 vqvaedfgdk qfshmrvrmp apeetvskts icssdlpgdk psplrdaykg slsasrqess
     5761 qagkpplpsa lggdsslclv heattdltls kagppnqtnk dittfdrqpv reqsptlrss
     5821 qsqmlasglq mgivagsgil qegikaspqs ailetfsssa temtglespe irglpseevh
     5881 agsaeeakgi dagrigavla aapvailpvr diisgkqlem ldiaqvsdep nifegcvelp
     5941 sqaesqtcpk ektimkteya diqhklssaf geslapvesk sgvtqgvpke vlealdrssq
     6001 eliasklded serdtnilsa tqgsiphdvf pdhiestldn saavpmrgay stpsstavcl
     6061 tperleiqat revaeerqek pevlvaleay rkstlrrsge dsydrnleef itvleeyepl
     6121 pagqlsdwkr aspafepsic hnseitsklp pledkkelet vgsildpsen vsellgnhaq
     6181 sqmlcedtgl ltdelgrtag hyvistaesd rgeclptlse qapgkereec lnydsalgvr
     6241 stsglessll eesheersld larsedeqps lvedsmnvpt essvidyqvv ghsgvvkdaq
     6301 tqpkdsdfdl heisrtgdkt dlplscgddg kksaefsktv sgiaadmvik dtdsehlsqd
     6361 mlhptastvl cegrsdvpys sdedqhkels rykevelvip flrgtsssle devedggsfs
     6421 sgdlqaekia eflttdpdtl nkvvhfpqif qdydtsahqe pfvptetemn vekhvgfvld
     6481 emaacstpcv plksdrqtei eeasrivesv vhiasslves lhevnhetai kedicdsala
     6541 yaagsplers evsetlsqag ppstiekdqr espfgvkemy qvlkesrgse driqysvtsf
     6601 gdplfadegd edqerrrrgs ptaeslddqa qiekcshevm mqlldgfsqs dvelaevrte
     6661 deapdieddv pspeagshee ksvpssqdns gdlqpqlste veigsqasga paplseldye
     6721 lcpdadqlrt sgdeavatrv ppleperdea vaesshapsa aadpaasagr naggrerspl
     6781 leasppeeta ycpeqdispa qeypeyltpd mqvrvgvspt eaqpakekpv rysessvess
     6841 sevgspcpps qggtamafen vafagedvid atsspieess spagsrspye vvrdahaeea
     6901 aqtalveaek slrhavqeev rarpqslvte esseqqehqi qpsifneafd hspeesapek
     6961 kqlsegllgv glltvsdtta rtpesrrgds dedeeewadh vmskirgrpi pptppaspra
     7021 vqsavevqge pweapvveqe rvnvesardm saerdagvdv mgdrsatqpq lqefrrtadg
     7081 krpqlehips eisdedlver eqspthitat crgdrkdsss drnsspdsdg pkasgshrrs
     7141 asgvgaiale avhsqdsggn ekhshesrdk gaqeddkite epvdtmfvie eeapadsnrs
     7201 esfcddader aartagglsp slesppqkkd lddgsssgkp daaspvvlrs kskrtdkisd
     7261 krssrdldtg hsgsetdhsh yysfeltsds gktpgasrpt ssefdvnils gqasseyetc
     7321 atsqdgevsg syataaassh dtsfatarss lsgssrssay svdsessghl gsmevsease
     7381 tivasaheel dsdiesersk svlrepydge ipesvirggv ehpflpswqp alkdssptlh
     7441 gsagarppta egvfspfqst vpsepgrscv gwpssslsta esvrspskpe vlngvsaple
     7501 gqrrlrhndd fmassheeeq lsvsessatq sehtwqtsve tmvdaveaqr fscdserhhr
     7561 qstmqnsvsl pfdeavvaed shreadddra tppfqevngp veveyvpeyd dltartlrpv
     7621 rqqnapdgqt eaqgdvalle asagdastsr eqheeenste lreegfyaet fqgdlsseev
     7681 esaanealll dksacsfyey chakdeyevc ntrpavesas lkeaferpat pepvdeiddq
     7741 edegnlaqgs pnlhgkdyea fghasaeesv pcsdkthiaq elsendqykv fsdsgnqssl
     7801 hdetrsksvs ssleramtdd vrlytskced yilqmhqtyk ygvglpktsd ggylpeegmd
     7861 venvkllesr lqegnggqqd fpydlsyqqe yggdgrvehg dlythqevee egyddersrt
     7921 rgrrrlspis dergstpdyd tlagrktfgk gsekddasts slmeferlea emasgtksss
     7981 vgssdslsgr qhqgrsgags eqdsvsvnsl teferlekei veaegvdarl rgevaaklde
     8041 ideghesqas dpghetavyd esgatlllca sadvneepdg ttddaqlgdi eaaqgqevaw
     8101 srterhfltt rtesghsves vdrqssasta tqfdadslre reiddsesnf gpdtpgwmqk
     8161 ecvdldslhd slhdsehhds lqevpskdkd slqlrepsad adslqedhne lqdslyetrd
     8221 idwdsfrade tsglddtsrs whqfpsqirt telsptlsrp assplvvpvv veridtltwv
     8281 dqdpakacvs dgeslgsrqq ehprndalvs stdslepsss anthatyqcd tdsmmsslns
     8341 aeestmvtst dtlehetlcl asqgkslsle gglfsggdvt eeqntrtple ggvsvddmee
     8401 rfrecllrsa asptneeqve erqtmdekgn livtrtvkrh vtseprlhtq tfmgpdaeqr
     8461 srefvasfaa mepsaerdey egfdaagntv rvtqhvvvrp tiktvtftgp darlqmreym
     8521 rslaggqhss aentptsdsg vgacsslasp pplmpdppaa apssacpglq rlsggdqwme
     8581 aesalddcsp pvtatrvitt rttrtmgpdg reqlvtttte esggeepear lrrsmqgvld
     8641 sfmaepptpp hldeee
//
LOCUS       XP_077503815            8655 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X23 [Amblyomma
            americanum].
ACCESSION   XP_077503815
VERSION     XP_077503815.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647689.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8655
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..8655
                     /product="uncharacterized protein LOC144114148 isoform
                     X23"
                     /calculated_mol_wt=929202
     Region          12..43
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          26..343
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(45,47,51..52,55..57,59..60,64,67,76,78,80,84..85,
                     88..90,92..93,97,100,109,111,113,117..118,121..123,
                     125..126,130,133)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          45..76
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          78..109
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          111..136
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          144..169
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          177..204
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(206,208,212..213,216..218,220..221,225,228,237,239,
                     241,245..246,249..251,253..254,258,261,270,272,274,
                     278..279,282..284,286..287,291,294)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          206..237
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          239..270
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          272..302
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          286..574
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          305..369
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          371..402
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(404,406,410..411,414..416,418..419,423,426,435,437,
                     439,443..444,447..449,451..452,456,459,468,470,472,
                     476..477,480..482,484..485,489,492)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          404..435
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          437..468
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          452..730
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          470..499
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          503..528
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          536..567
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          569..602
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          603..633
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          635..665
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(668,670,674..675,678..680,682..683,687,690,699,701,
                     703,707..708,711..713,715..716,720,723,732,734,736,
                     740..741,744..746,748..749,753,756)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          668..699
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          701..732
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          706..787
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          734..764
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          918..1021
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1244..1376
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1408..1491
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <1804..2055
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          <4418..4678
                     /region_name="YbbR"
                     /note="Cyclic di-AMP synthase regulator CdaR, YbbR domain
                     [Signal transduction mechanisms]; COG4856"
                     /db_xref="CDD:443884"
     Region          <7083..7396
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..8655
                     /gene="LOC144114148"
                     /coded_by="XM_077647689.1:359..26326"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 mgaaqrklak tdgnasflra aragnlekvl dylkgsidin tsnanglnal hlaakeghvn
       61 vvsellkrga nvnattkkgn salhiaslag qeevvkllvq kqanvnvqsq ngftplymaa
      121 qenhdgvvrf llanganqsl atedgftpla valqqghdkv vavllendar gkvrlpalhi
      181 askkddckaa alllhsehnp dvtsksgftp lhiaahygns niasllldkg advnfpakhq
      241 itplhvaakw gksnmvklll ekgakidast rdgltplhca arsghdqvve qlleknapit
      301 aktknglapl hmasqgdhvd sarillyhka pvddvtvdyl talhvaahcg hvgvakllld
      361 rradpnaral ngftplhiac kknrikvvel llkhgasiea ttesgltplh vasfmgcmni
      421 viyliqhgan pdiptvrget plhlaaranq tdiirillrn gahvdakare lqtalhiasr
      481 lgnadmvgll lqhgaavdap tkdaytplhv aaregqdeva allldhgaal aaptkkgftp
      541 lhlaakygnl kvaqlllqkd apvdaqgkng vtplhvaahy dhvnvallll ekgasphsaa
      601 rngytplhva arkdqmdias slleygakpg aesragftpl hlaaqeghad laallvehga
      661 dcdakakngl tpmhlcaqed rvevatilak hgasldpttk agytplhvac hfgqtnmirf
      721 llrqganvna ttshgytplh qaaqqghtli inlllehraa pnavtnqgqt alaiaqrlgy
      781 isvvetlkvv tetivttttt tvteekyrvv apetmhetfm sdsedeaaed nmlgdqsfry
      841 ltademkslg ddslpidvtr deritesihi trepghpapl tqeeerlspt qahtteaifv
      901 gnyapdnvdl srtpihagfl vsflvdargg amrgcrhsgv rviipprkaq mpmritcryl
      961 rkeklphppp llegeacasr ilevgpagak flgpvilevp hfaslrgker eisilrsdng
     1021 etwkehtlea seeavqevln esfegeelsa ledlqtnriv rilttdfpqy favvsrtrqe
     1081 vhavgpeggl lsstvvpqvq aifpegaltk kirvglqaqp ipaelvtkll ghrvavspiv
     1141 tveprrrkfh kpitltipvp qaatkgminq yagdaptlrl lcsitggtnk aqwedvtgst
     1201 pltfvndcvs ftttvsarfw lmdcrqvsea tkfatelyae amhvpfmakf vvfakrqdpm
     1261 eaqlrvfcmt ddkedktles qehftevaks rdvevlekks qylefggnlv pvtksgeqlq
     1321 lqfqafrenr lpfavrvkdp hqeplgrlaf mrqpraarat eppqaplcnl nialpeyeas
     1381 qnlselvtle kkygfveetg lakpelihra dlrlsdiare lgsdwpalaa qldvpeqdva
     1441 sirtecpsdl aqqallmlrl wmkraggrat gnnlekglrm inredivnkc mfnvelvtdd
     1501 vekavakvhl dqsgfdtfre elgtpkdtsl krdasldvsy deqdlmkaes aeetssetgs
     1561 vhekhtgsea kgtskaipgv rkgspisaai grivrgsske kaaprmavee tfeeepvlki
     1621 dsaevtqqaa peagptappr dskkkskkek spsplgpdys lprmrdyhtp erdftsttdt
     1681 livssikedv daskelatdg kvkkekkkek kekspsppgp dytlprlrdt ytppsdvtaa
     1741 gyaastlpgq gitleqgivk eplerksker slspgvvktg engslgadps verqvkqlsq
     1801 gpedtiyekv ervvketpgk ivemvhikrq rsskspegrg spkdknkglp ssevvapdis
     1861 gesrsvpset igaekkqkkh ketsptkqgk keakevseav dkdaelsasd gklpdsgfls
     1921 kmaklpkkmf prsgkasasd eepsslsess pvakkkagkl pksatlpkak psktplyeik
     1981 svdvevdtsk ltskgvkgvp akdgeglets rdkepdsrff skmaripkkm fprssktsss
     2041 edepssasen lpsskqrakk ekeisskkke kvvkvkpdsk klstsfevev drdtlpgagv
     2101 dinakkpglq eslesesrkk tdsmqisfpq svkavgskge pmeysldieg mkslqkepql
     2161 avvgefpgkp spdielslts ketapefsdk arghieigtp ehgksskggf mtkmaklprk
     2221 mfphgfkgdv sveepsveae svhvsvpeaq vklgtkdlkk kgpdmpamel taslpvgaeg
     2281 svemsapdlp vsadgglisk vtqfpkmfsh dsqdgvtier ppvvgegidv esslpdvqlk
     2341 vkgkafekpe tkfdvtlper kvtpvlpdga eghievnlde mgksyddgfm skmaklpkkm
     2401 fshgledeia vqepsitqhd ihvdipklkd eleiratlpe kpqvdfdvai pgkqvsldlp
     2461 egveskievs apdvgkptdg gfmskvvqlp kkmfphgskg tvpleeapea vgdihakvpe
     2521 ahlkvglkgl ekpdfegdia vpgkkgavsl pegtegkiev stpetgkpsd kgfmskmakl
     2581 pkkmfphgsk gsvsveevpe vdrsvkvkvp qgkipeahlk vdikdpekhk dvdialseek
     2641 vdidlpegid knievtmpev skpseggfis kmakfpkkmf phgakgsvsv eeapgidvdv
     2701 kmpetrismd vkglekpkge vdislpkekv didvpkgfdg klemsapevg kpseggfmsk
     2761 makfpkkmfp hgakgsvsve eapgidvdvk vpeahismdv kglekprgev dislpkekvd
     2821 idvpkgfdgk lemsapevgk pseggfmskm akfpkkmfph gakgslsvee tpgidvdvkm
     2881 peadlsmdvk glekpkaeld iampkgkidi dmpegvdgkl easapdigkp sdggfiskmt
     2941 kfpkkmfphg akgtvsveap elgdvqvnmp eahlgvdikg pekpkgeldv tlpkgkvdid
     3001 lpegiegkve isapeigkpd ggfmskmakl pkkmfphgak ggvsvegspe lgdvevkmpe
     3061 ahlgvdikgp ekpkgeldvt lpkgkvdidl pegiegkvei sapeigkpdg gfmskmaklp
     3121 kkmfphgakg gvsvegspel gdvevkmpea hlgvdikgpe kpkgeldvtl pkgkvdidlp
     3181 egiegkveis apeigkpdgg fmskmaklpk kmfphgakgg vsvegspelg dvevkmpeah
     3241 lgvdikgpek pkgeldvtlp kgkvdidlpe giegkveisa peigkpdggf mskmaklpkk
     3301 mfshgakggv svegspelgd vqvkmpeahl gvdikgpekp kgeldvtlpk gkvdidlpeg
     3361 iegkveissp eigkpdggfm skmaklpkkm fphgakggvs vegapelgdv evkmpeahlg
     3421 vdikgpekpk geldvtlpkg kvdidlpegi egkveisape igkpdggfms kmaklpkkmf
     3481 phgakggvsv egapelgdve vkmpeahlgv dikgpekpkg eldvtlpkgk vdidlpegfe
     3541 gkveisapei gkpdggfmsk maklpkkmfp hgtkggvsve gapelgdvev kmpeahlgvd
     3601 ikgpekpkge ldvtlpkgkv didlpegieg kveisspeig kpdggfmskm aklpkkmfph
     3661 gakggvsveg apelgdvevk mpeahlgvdi kgpekpkgel dvtlpkgkvd idlpegiegk
     3721 veisapeigk pdggfmskma klpkkmfphg akggvsvegs pelgdvevkm peahlgvdik
     3781 gpekpkgeld vtlpkgkvdi dlpegiegkl eisapeigkp dggfmskmak fpkkmfphga
     3841 kggvsvegap elgdvqvkmp eahlgvdikg pekpkgeldv tlpkgkvdid lpegiegkve
     3901 isapeigkpd ggfmskmakf pkkmfphgak ggvsvegape lgdvqvkmpe ahlgvdiegp
     3961 ekpkgeldvt lpkgkvdidl pegiegkvei sapeigkpdg gfmskmakfp kkmfphgtkg
     4021 gvsvegapel gdvevkmpea hlgvdikgpe kpkgeldvtl pkgkvdidlp egiegkveis
     4081 apeigkpdgg fmskmaklpq kmfphgtkgg vsvegapelg diqlkmpeah lgvdikgpek
     4141 pkgeldamlp kgkvdidlpe giegkveisa peigkpdggf mskmaklpkk mfshgakgsi
     4201 svegspefgd vevkmpeahl ggdikgpekp kgeldvalpk gkvdidipeg iegkveisap
     4261 eigkpsdggf vskmaklpkk mfphgtkgsv siegapevgd vqvkvpeaql kmdikgtgkp
     4321 efegeitwtg kkvavelpeg iegtvemstp emgrpsdggf mskvaklpkk vfshgskggv
     4381 pfdeetgvag dievkmseaa lkvdvkypek pesdfsalek kididvpegf kgkveistpe
     4441 lgkpsdggfl skmsklpkkv lphgskgeis agevsevsge vpvelpearl kvgvkgperp
     4501 dsdvdisvsd kkvtldvtkg fegkvevstp dlgkpsdkgf iskvtkfpkk mfphgskggv
     4561 svgevsevtg dvhvelpdah lkvgvkgper pdsdvdvsma vkkitvdvpe gfegklevst
     4621 pelarspdgs lvskaaklpk klvpsgensl ssveepslsa hvdvpgsdtt fkvdaklpam
     4681 pvtdvdisls akessadipv gvagkveigt pdagkpsdkg fmskmakipk kmfhhgpkss
     4741 gstdehstva etvddkalgf evqlqkdvki ptkpeadvsv sfhekevtvq lpegregtve
     4801 lstpglvgeh vpdkssqgad vllpgkelpt vegqeiiekp ggvievievt ahelskplss
     4861 gllskvskip kkifprgsks kssdedspsp gkrelketpg givevvevtk hdvaihpest
     4921 hvkqvgtevl lepatsetkp sgaeglppth itreikevpg givevvrvtk hivgklpedk
     4981 gigepsvrtt daslpweael akdpsavikr evketpeghv evvqvirrea sdvpfgsdfp
     5041 gdlesdgtvs rriettvvdg kptvirrlvt ttsdgirqvt ehieqgaeda gsaasslfak
     5101 mgefakglss ddpsavttre vkqlpggave vvsvtkrsss speteitsqe qlqsstsgsm
     5161 hfaesavgkp meakttiirr eisgpetivr tttsvvtsat apgsskpels advldkmfga
     5221 mekprvddrl rdvltaeggy vakrevtpee aellaqlfpe qstgvavypp aglvpeaaii
     5281 peyrcmttae gahhdpeske kvllklgral sqddldapsp tqgeaamtva glgeavdtsd
     5341 qveepavekl elarreskry sqefeqdepl pkamgfktkp seetvvsstq vkgvhdngtv
     5401 khtvttvtkt tiiqeveepa gaprpgeset wasdldtsqv svsslpeder teptlsssat
     5461 tatadaeals gplartqvee kewtetdpds gevrhiiskt thttvtskkp segaprgdtt
     5521 sttvshsskd ghhgstpsap secdeaeslt ffqkkeffek lseqsppket ssarlgstps
     5581 ldtaltrpav tlkprprses ltsqgeiiee sivfaerlrl fqngaqaspl psasipqvdl
     5641 vdpapkmeqk lqgmaeigds lqqvpsavle glqsteeksd trllvhtsda dkeafdvrsv
     5701 qvaedfgdkq fshmrvrmpa peetvsktsi cssdlpgdkp splrdaykgs lsasrqessq
     5761 agkpplpsal ggdsslclvh eattdltlsk agppnqtnkd ittfdrqpvr eqsptlrssq
     5821 sqmlasglqm givagsgilq egikaspqsa iletfsssat emtglespei rglpseevha
     5881 gsaeeakgid agrigavlaa apvailpvrd iisgkqleml diaqvsdepn ifegcvelps
     5941 qaesqtcpke ktimkteyad iqhklssafg eslapvesks gvtqgvpkev lealdrssqe
     6001 liaskldeds erdtnilsat qgsiphdvfp dhiestldns aavpmrgays tpsstavclt
     6061 perleiqatr evaeerqekp evlvaleayr kstlrrsged sydrnleefi tvleeyeplp
     6121 agqlsdwkra spafepsich nseitsklpp ledkkeletv gsildpsenv sellgnhaqs
     6181 qmlcedtgll tdelgrtagh yvistaesdr geclptlseq apgkereecl nydsalgvrs
     6241 tsglesslle esheersldl arsedeqpsl vedsmnvpte ssvidyqvvg hsgvvkdaqt
     6301 qpkdsdfdlh eisrtgdktd lplscgddgk ksaefsktvs giaadmvikd tdsehlsqdm
     6361 lhptastvlc egrsdvpyss dedqhkelsr ykevelvipf lrgtsssled evedggsfss
     6421 gdlqaekiae flttdpdtln kvvhfpqifq dydtsahqep fvptetemnv ekhvgfvlde
     6481 maacstpcvp lksdrqteie easrivesvv hiasslvesl hevnhetaik edicdsalay
     6541 aagsplerse vsetlsqagp pstiekdqre spfgvkemyq vlkesrgsed riqysvtsfg
     6601 dplfadegde dqerrrrgsp taeslddqaq iekcshevmm qlldgfsqsd velaevrted
     6661 eapdieddvp speagsheek svpssqdnsg dlqpqlstev eigsqasgap aplseldyel
     6721 cpdadqlrts gdeavatrvp pleperdeav aesshapsaa adpaasagrn aggrerspll
     6781 easppeetay cpeqdispaq eypeyltpdm qvrvgvspte aqpakekpvr ysessvesss
     6841 evgspcppsq ggtamafenv afagedvida tsspieesss pagsrspyev vrdahaeeaa
     6901 qtalveaeks lrhavqeevr arpqslvtee sseqqehqiq psifneafdh speesapekk
     6961 qlsegllgvg lltvsdttar tpesrrgdsd edeeewadhv mskirgrpip ptppasprav
     7021 qsavevqgep weapvveqer vnvesardms aerdagvdvm gdrsatqpql qefrrtadgk
     7081 rpqlehipse isdedlvere qspthitatc rgdrkdsssd rnsspdsdgp kasgshrrsa
     7141 sgvgaialea vhsqdsggne khshesrdkg aqeddkitee pvdtmfviee eapadsnrse
     7201 sfcddadera artagglsps lesppqkkdl ddgsssgkpd aaspvvlrsk skrtdkisdk
     7261 rssrdldtgh sgsetdhshy ysfeltsdsg ktpgasrpts sefdvnilsg qasseyetca
     7321 tsqdgevsgs yataaasshd tsfatarssl sgssrssays vdsessghlg smevseaset
     7381 ivasaheeld sdiesersks vlrepydgei pesvirggve hpflpswqpa lkdssptlhg
     7441 sagarpptae gvfspfqstv psepgrscvg wpssslstae svrspskpev lngvsapleg
     7501 qrrlrhnddf massheeeql svsessatqs ehtwqtsvet mvdaveaqrf scdserhhrq
     7561 stmqnsvslp fdeavvaeds hreadddrat ppfqevngpv eveyvpeydd ltartlrpvr
     7621 qqnapdgqte aqgdvallea sagdastsre qheeenstel reegfyaetf qgdlsseeve
     7681 saaneallld ksacsfyeyc hakdeyevcn trpavesasl keaferpatp epvdeiddqe
     7741 degnlaqgsp nlhgkdyeaf ghasaeesvp csdkthiaqe lsendqykvf sdsgnqsslh
     7801 detrsksvss sleramtddv rlytskcedy ilqmhqtyky gvglpktsdg gylpeegmdv
     7861 envkllesrl qegnggqqdf pydlsyqqey ggdgrvehgd lythqeveee gyddersrtr
     7921 grrrlspisd ergstpdydt lagrktfgkg sekddastss lmeferleae masgtksssv
     7981 gssdslsgrq hqgrsgagse qdsvsvnslt eferlekeiv eaegvdarlr gevaakldei
     8041 deghesqasd pghetavyde sgatlllcas advneepdgt tddaqlgdie aaqgqevaws
     8101 rterhflttr tesghsvesv drqssastat qfdadslrer eiddsesnfg pdtpgwmqke
     8161 cvdldslhds lhdsehhdsl qevpskdkds lqlrepsada dslqedhnel qdslyetrdi
     8221 dwdsfradet sglddtsrsw hqfpsqirtt elsptlsrpa ssplvvpvvv eridtltwvd
     8281 qdpakacvsd geslgsrqqe hprndalvss tdslepsssa nthatyqcdt dsmmsslnsa
     8341 eestmvtstd tlehetlcla sqgkslsleg glfsggdvte eqntrtpleg gvsvddmeer
     8401 frecllrsaa sptneeqvee rqtmdekgnl ivtrtvkrhv tseprlhtqt fmgpdaeqrs
     8461 refvasfaam epsaerdeye gfdaagntvr vtqhvvvrpt iktvtftgpd arlqmreymr
     8521 slaggqhssa entptsdsgv gacsslaspp plmpdppaaa pssacpglqr lsggdqwmea
     8581 esalddcspp vtatrvittr ttrtmgpdgr eqlvttttee sggeepearl rrsmqgvlds
     8641 fmaepptpph ldeee
//
LOCUS       XP_077503816            8653 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X24 [Amblyomma
            americanum].
ACCESSION   XP_077503816
VERSION     XP_077503816.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647690.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8653
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..8653
                     /product="uncharacterized protein LOC144114148 isoform
                     X24"
                     /calculated_mol_wt=929050
     Region          10..308
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          10..41
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(43,45,49..50,53..55,57..58,62,65,74,76,78,82..83,
                     86..88,90..91,95,98,107,109,111,115..116,119..121,
                     123..124,128,131)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          43..74
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          76..107
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          109..134
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          142..167
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          175..202
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(204,206,210..211,214..216,218..219,223,226,235,237,
                     239,243..244,247..249,251..252,256,259,268,270,272,
                     276..277,280..282,284..285,289,292)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          204..235
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          237..268
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          270..300
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          284..572
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          303..367
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          369..400
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(402,404,408..409,412..414,416..417,421,424,433,435,
                     437,441..442,445..447,449..450,454,457,466,468,470,
                     474..475,478..480,482..483,487,490)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          402..433
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          435..466
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          450..728
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          468..497
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          501..526
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          534..565
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          567..600
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          601..631
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          633..663
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(666,668,672..673,676..678,680..681,685,688,697,699,
                     701,705..706,709..711,713..714,718,721,730,732,734,
                     738..739,742..744,746..747,751,754)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          666..697
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          699..730
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          704..785
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          732..762
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          916..1019
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1242..1374
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1406..1489
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <1802..2053
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          <4416..4676
                     /region_name="YbbR"
                     /note="Cyclic di-AMP synthase regulator CdaR, YbbR domain
                     [Signal transduction mechanisms]; COG4856"
                     /db_xref="CDD:443884"
     Region          <7081..7394
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..8653
                     /gene="LOC144114148"
                     /coded_by="XM_077647690.1:271..26232"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 mvtegeqqtd gnasflraar agnlekvldy lkgsidints nanglnalhl aakeghvnvv
       61 sellkrganv nattkkgnsa lhiaslagqe evvkllvqkq anvnvqsqng ftplymaaqe
      121 nhdgvvrfll anganqslat edgftplava lqqghdkvva vllendargk vrlpalhias
      181 kkddckaaal llhsehnpdv tsksgftplh iaahygnsni asllldkgad vnfpakhqit
      241 plhvaakwgk snmvklllek gakidastrd gltplhcaar sghdqvveql leknapitak
      301 tknglaplhm asqgdhvdsa rillyhkapv ddvtvdylta lhvaahcghv gvakllldrr
      361 adpnaralng ftplhiackk nrikvvelll khgasieatt esgltplhva sfmgcmnivi
      421 yliqhganpd iptvrgetpl hlaaranqtd iirillrnga hvdakarelq talhiasrlg
      481 nadmvglllq hgaavdaptk daytplhvaa regqdevaal lldhgaalaa ptkkgftplh
      541 laakygnlkv aqlllqkdap vdaqgkngvt plhvaahydh vnvallllek gasphsaarn
      601 gytplhvaar kdqmdiassl leygakpgae sragftplhl aaqeghadla allvehgadc
      661 dakakngltp mhlcaqedrv evatilakhg asldpttkag ytplhvachf gqtnmirfll
      721 rqganvnatt shgytplhqa aqqghtliin lllehraapn avtnqgqtal aiaqrlgyis
      781 vvetlkvvte tivttttttv teekyrvvap etmhetfmsd sedeaaednm lgdqsfrylt
      841 ademkslgdd slpidvtrde ritesihitr epghpapltq eeerlsptqa htteaifvgn
      901 yapdnvdlsr tpihagflvs flvdarggam rgcrhsgvrv iipprkaqmp mritcrylrk
      961 eklphpppll egeacasril evgpagakfl gpvilevphf aslrgkerei silrsdnget
     1021 wkehtlease eavqevlnes fegeelsale dlqtnrivri lttdfpqyfa vvsrtrqevh
     1081 avgpegglls stvvpqvqai fpegaltkki rvglqaqpip aelvtkllgh rvavspivtv
     1141 eprrrkfhkp itltipvpqa atkgminqya gdaptlrllc sitggtnkaq wedvtgstpl
     1201 tfvndcvsft ttvsarfwlm dcrqvseatk fatelyaeam hvpfmakfvv fakrqdpmea
     1261 qlrvfcmtdd kedktlesqe hftevaksrd vevlekksqy lefggnlvpv tksgeqlqlq
     1321 fqafrenrlp favrvkdphq eplgrlafmr qpraaratep pqaplcnlni alpeyeasqn
     1381 lselvtlekk ygfveetgla kpelihradl rlsdiarelg sdwpalaaql dvpeqdvasi
     1441 rtecpsdlaq qallmlrlwm kraggratgn nlekglrmin redivnkcmf nvelvtddve
     1501 kavakvhldq sgfdtfreel gtpkdtslkr dasldvsyde qdlmkaesae etssetgsvh
     1561 ekhtgseakg tskaipgvrk gspisaaigr ivrgsskeka aprmaveetf eeepvlkids
     1621 aevtqqaape agptapprds kkkskkeksp splgpdyslp rmrdyhtper dftsttdtli
     1681 vssikedvda skelatdgkv kkekkkekke kspsppgpdy tlprlrdtyt ppsdvtaagy
     1741 aastlpgqgi tleqgivkep lerkskersl spgvvktgen gslgadpsve rqvkqlsqgp
     1801 edtiyekver vvketpgkiv emvhikrqrs skspegrgsp kdknkglpss evvapdisge
     1861 srsvpsetig aekkqkkhke tsptkqgkke akevseavdk daelsasdgk lpdsgflskm
     1921 aklpkkmfpr sgkasasdee psslsesspv akkkagklpk satlpkakps ktplyeiksv
     1981 dvevdtsklt skgvkgvpak dgegletsrd kepdsrffsk maripkkmfp rssktsssed
     2041 epssasenlp sskqrakkek eisskkkekv vkvkpdskkl stsfevevdr dtlpgagvdi
     2101 nakkpglqes lesesrkktd smqisfpqsv kavgskgepm eysldiegmk slqkepqlav
     2161 vgefpgkpsp dielsltske tapefsdkar ghieigtpeh gksskggfmt kmaklprkmf
     2221 phgfkgdvsv eepsveaesv hvsvpeaqvk lgtkdlkkkg pdmpamelta slpvgaegsv
     2281 emsapdlpvs adggliskvt qfpkmfshds qdgvtierpp vvgegidves slpdvqlkvk
     2341 gkafekpetk fdvtlperkv tpvlpdgaeg hievnldemg ksyddgfmsk maklpkkmfs
     2401 hgledeiavq epsitqhdih vdipklkdel eiratlpekp qvdfdvaipg kqvsldlpeg
     2461 veskievsap dvgkptdggf mskvvqlpkk mfphgskgtv pleeapeavg dihakvpeah
     2521 lkvglkglek pdfegdiavp gkkgavslpe gtegkievst petgkpsdkg fmskmaklpk
     2581 kmfphgskgs vsveevpevd rsvkvkvpqg kipeahlkvd ikdpekhkdv dialseekvd
     2641 idlpegidkn ievtmpevsk pseggfiskm akfpkkmfph gakgsvsvee apgidvdvkm
     2701 petrismdvk glekpkgevd islpkekvdi dvpkgfdgkl emsapevgkp seggfmskma
     2761 kfpkkmfphg akgsvsveea pgidvdvkvp eahismdvkg lekprgevdi slpkekvdid
     2821 vpkgfdgkle msapevgkps eggfmskmak fpkkmfphga kgslsveetp gidvdvkmpe
     2881 adlsmdvkgl ekpkaeldia mpkgkididm pegvdgklea sapdigkpsd ggfiskmtkf
     2941 pkkmfphgak gtvsveapel gdvqvnmpea hlgvdikgpe kpkgeldvtl pkgkvdidlp
     3001 egiegkveis apeigkpdgg fmskmaklpk kmfphgakgg vsvegspelg dvevkmpeah
     3061 lgvdikgpek pkgeldvtlp kgkvdidlpe giegkveisa peigkpdggf mskmaklpkk
     3121 mfphgakggv svegspelgd vevkmpeahl gvdikgpekp kgeldvtlpk gkvdidlpeg
     3181 iegkveisap eigkpdggfm skmaklpkkm fphgakggvs vegspelgdv evkmpeahlg
     3241 vdikgpekpk geldvtlpkg kvdidlpegi egkveisape igkpdggfms kmaklpkkmf
     3301 shgakggvsv egspelgdvq vkmpeahlgv dikgpekpkg eldvtlpkgk vdidlpegie
     3361 gkveisspei gkpdggfmsk maklpkkmfp hgakggvsve gapelgdvev kmpeahlgvd
     3421 ikgpekpkge ldvtlpkgkv didlpegieg kveisapeig kpdggfmskm aklpkkmfph
     3481 gakggvsveg apelgdvevk mpeahlgvdi kgpekpkgel dvtlpkgkvd idlpegfegk
     3541 veisapeigk pdggfmskma klpkkmfphg tkggvsvega pelgdvevkm peahlgvdik
     3601 gpekpkgeld vtlpkgkvdi dlpegiegkv eisspeigkp dggfmskmak lpkkmfphga
     3661 kggvsvegap elgdvevkmp eahlgvdikg pekpkgeldv tlpkgkvdid lpegiegkve
     3721 isapeigkpd ggfmskmakl pkkmfphgak ggvsvegspe lgdvevkmpe ahlgvdikgp
     3781 ekpkgeldvt lpkgkvdidl pegiegklei sapeigkpdg gfmskmakfp kkmfphgakg
     3841 gvsvegapel gdvqvkmpea hlgvdikgpe kpkgeldvtl pkgkvdidlp egiegkveis
     3901 apeigkpdgg fmskmakfpk kmfphgakgg vsvegapelg dvqvkmpeah lgvdiegpek
     3961 pkgeldvtlp kgkvdidlpe giegkveisa peigkpdggf mskmakfpkk mfphgtkggv
     4021 svegapelgd vevkmpeahl gvdikgpekp kgeldvtlpk gkvdidlpeg iegkveisap
     4081 eigkpdggfm skmaklpqkm fphgtkggvs vegapelgdi qlkmpeahlg vdikgpekpk
     4141 geldamlpkg kvdidlpegi egkveisape igkpdggfms kmaklpkkmf shgakgsisv
     4201 egspefgdve vkmpeahlgg dikgpekpkg eldvalpkgk vdidipegie gkveisapei
     4261 gkpsdggfvs kmaklpkkmf phgtkgsvsi egapevgdvq vkvpeaqlkm dikgtgkpef
     4321 egeitwtgkk vavelpegie gtvemstpem grpsdggfms kvaklpkkvf shgskggvpf
     4381 deetgvagdi evkmseaalk vdvkypekpe sdfsalekki didvpegfkg kveistpelg
     4441 kpsdggflsk msklpkkvlp hgskgeisag evsevsgevp velpearlkv gvkgperpds
     4501 dvdisvsdkk vtldvtkgfe gkvevstpdl gkpsdkgfis kvtkfpkkmf phgskggvsv
     4561 gevsevtgdv hvelpdahlk vgvkgperpd sdvdvsmavk kitvdvpegf egklevstpe
     4621 larspdgslv skaaklpkkl vpsgenslss veepslsahv dvpgsdttfk vdaklpampv
     4681 tdvdislsak essadipvgv agkveigtpd agkpsdkgfm skmakipkkm fhhgpkssgs
     4741 tdehstvaet vddkalgfev qlqkdvkipt kpeadvsvsf hekevtvqlp egregtvels
     4801 tpglvgehvp dkssqgadvl lpgkelptve gqeiiekpgg vievievtah elskplssgl
     4861 lskvskipkk ifprgsksks sdedspspgk relketpggi vevvevtkhd vaihpesthv
     4921 kqvgtevlle patsetkpsg aeglppthit reikevpggi vevvrvtkhi vgklpedkgi
     4981 gepsvrttda slpweaelak dpsavikrev ketpeghvev vqvirreasd vpfgsdfpgd
     5041 lesdgtvsrr iettvvdgkp tvirrlvttt sdgirqvteh ieqgaedags aasslfakmg
     5101 efakglssdd psavttrevk qlpggavevv svtkrssssp eteitsqeql qsstsgsmhf
     5161 aesavgkpme akttiirrei sgpetivrtt tsvvtsatap gsskpelsad vldkmfgame
     5221 kprvddrlrd vltaeggyva krevtpeeae llaqlfpeqs tgvavyppag lvpeaaiipe
     5281 yrcmttaega hhdpeskekv llklgralsq ddldapsptq geaamtvagl geavdtsdqv
     5341 eepaveklel arreskrysq efeqdeplpk amgfktkpse etvvsstqvk gvhdngtvkh
     5401 tvttvtktti iqeveepaga prpgesetwa sdldtsqvsv sslpederte ptlsssatta
     5461 tadaealsgp lartqveeke wtetdpdsge vrhiisktth ttvtskkpse gaprgdttst
     5521 tvshsskdgh hgstpsapse cdeaesltff qkkeffekls eqsppketss arlgstpsld
     5581 taltrpavtl kprprseslt sqgeiieesi vfaerlrlfq ngaqasplps asipqvdlvd
     5641 papkmeqklq gmaeigdslq qvpsavlegl qsteeksdtr llvhtsdadk eafdvrsvqv
     5701 aedfgdkqfs hmrvrmpape etvsktsics sdlpgdkpsp lrdaykgsls asrqessqag
     5761 kpplpsalgg dsslclvhea ttdltlskag ppnqtnkdit tfdrqpvreq sptlrssqsq
     5821 mlasglqmgi vagsgilqeg ikaspqsail etfsssatem tglespeirg lpseevhags
     5881 aeeakgidag rigavlaaap vailpvrdii sgkqlemldi aqvsdepnif egcvelpsqa
     5941 esqtcpkekt imkteyadiq hklssafges lapvesksgv tqgvpkevle aldrssqeli
     6001 askldedser dtnilsatqg siphdvfpdh iestldnsaa vpmrgaystp sstavcltpe
     6061 rleiqatrev aeerqekpev lvaleayrks tlrrsgedsy drnleefitv leeyeplpag
     6121 qlsdwkrasp afepsichns eitsklpple dkkeletvgs ildpsenvse llgnhaqsqm
     6181 lcedtglltd elgrtaghyv istaesdrge clptlseqap gkereeclny dsalgvrsts
     6241 glessllees heersldlar sedeqpslve dsmnvptess vidyqvvghs gvvkdaqtqp
     6301 kdsdfdlhei srtgdktdlp lscgddgkks aefsktvsgi aadmvikdtd sehlsqdmlh
     6361 ptastvlceg rsdvpyssde dqhkelsryk evelvipflr gtsssledev edggsfssgd
     6421 lqaekiaefl ttdpdtlnkv vhfpqifqdy dtsahqepfv ptetemnvek hvgfvldema
     6481 acstpcvplk sdrqteieea srivesvvhi asslveslhe vnhetaiked icdsalayaa
     6541 gsplersevs etlsqagpps tiekdqresp fgvkemyqvl kesrgsedri qysvtsfgdp
     6601 lfadegdedq errrrgspta eslddqaqie kcshevmmql ldgfsqsdve laevrtedea
     6661 pdieddvpsp eagsheeksv pssqdnsgdl qpqlstevei gsqasgapap lseldyelcp
     6721 dadqlrtsgd eavatrvppl eperdeavae sshapsaaad paasagrnag grerspllea
     6781 sppeetaycp eqdispaqey peyltpdmqv rvgvspteaq pakekpvrys essvesssev
     6841 gspcppsqgg tamafenvaf agedvidats spieessspa gsrspyevvr dahaeeaaqt
     6901 alveaekslr havqeevrar pqslvteess eqqehqiqps ifneafdhsp eesapekkql
     6961 segllgvgll tvsdttartp esrrgdsded eeewadhvms kirgrpippt ppaspravqs
     7021 avevqgepwe apvveqervn vesardmsae rdagvdvmgd rsatqpqlqe frrtadgkrp
     7081 qlehipseis dedlvereqs pthitatcrg drkdsssdrn sspdsdgpka sgshrrsasg
     7141 vgaialeavh sqdsggnekh shesrdkgaq eddkiteepv dtmfvieeea padsnrsesf
     7201 cddaderaar tagglspsle sppqkkdldd gsssgkpdaa spvvlrsksk rtdkisdkrs
     7261 srdldtghsg setdhshyys feltsdsgkt pgasrptsse fdvnilsgqa sseyetcats
     7321 qdgevsgsya taaasshdts fatarsslsg ssrssaysvd sessghlgsm evseasetiv
     7381 asaheeldsd iesersksvl repydgeipe svirggvehp flpswqpalk dssptlhgsa
     7441 garpptaegv fspfqstvps epgrscvgwp ssslstaesv rspskpevln gvsaplegqr
     7501 rlrhnddfma ssheeeqlsv sessatqseh twqtsvetmv daveaqrfsc dserhhrqst
     7561 mqnsvslpfd eavvaedshr eadddratpp fqevngpvev eyvpeyddlt artlrpvrqq
     7621 napdgqteaq gdvalleasa gdastsreqh eeenstelre egfyaetfqg dlsseevesa
     7681 aneallldks acsfyeycha kdeyevcntr pavesaslke aferpatpep vdeiddqede
     7741 gnlaqgspnl hgkdyeafgh asaeesvpcs dkthiaqels endqykvfsd sgnqsslhde
     7801 trsksvsssl eramtddvrl ytskcedyil qmhqtykygv glpktsdggy lpeegmdven
     7861 vkllesrlqe gnggqqdfpy dlsyqqeygg dgrvehgdly thqeveeegy ddersrtrgr
     7921 rrlspisder gstpdydtla grktfgkgse kddastsslm eferleaema sgtksssvgs
     7981 sdslsgrqhq grsgagseqd svsvnsltef erlekeivea egvdarlrge vaakldeide
     8041 ghesqasdpg hetavydesg atlllcasad vneepdgttd daqlgdieaa qgqevawsrt
     8101 erhflttrte sghsvesvdr qssastatqf dadslrerei ddsesnfgpd tpgwmqkecv
     8161 dldslhdslh dsehhdslqe vpskdkdslq lrepsadads lqedhnelqd slyetrdidw
     8221 dsfradetsg lddtsrswhq fpsqirttel sptlsrpass plvvpvvver idtltwvdqd
     8281 pakacvsdge slgsrqqehp rndalvsstd slepsssant hatyqcdtds mmsslnsaee
     8341 stmvtstdtl ehetlclasq gkslsleggl fsggdvteeq ntrtpleggv svddmeerfr
     8401 ecllrsaasp tneeqveerq tmdekgnliv trtvkrhvts eprlhtqtfm gpdaeqrsre
     8461 fvasfaamep saerdeyegf daagntvrvt qhvvvrptik tvtftgpdar lqmreymrsl
     8521 aggqhssaen tptsdsgvga csslaspppl mpdppaaaps sacpglqrls ggdqwmeaes
     8581 alddcsppvt atrvittrtt rtmgpdgreq lvtttteesg geepearlrr smqgvldsfm
     8641 aepptpphld eee
//
LOCUS       XP_077503817            8565 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X25 [Amblyomma
            americanum].
ACCESSION   XP_077503817
VERSION     XP_077503817.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647691.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8565
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..8565
                     /product="uncharacterized protein LOC144114148 isoform
                     X25"
                     /calculated_mol_wt=920012
     Region          <1..259
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          27..52
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          60..87
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(89,91,95..96,99..101,103..104,108,111,120,122,124,
                     128..129,132..134,136..137,141,144,153,155,157,161..162,
                     165..167,169..170,174,177)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          89..120
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          122..153
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          155..185
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          169..457
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          188..252
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          254..285
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(287,289,293..294,297..299,301..302,306,309,318,320,
                     322,326..327,330..332,334..335,339,342,351,353,355,
                     359..360,363..365,367..368,372,375)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          287..318
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          320..351
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          335..613
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          353..382
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          386..411
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          419..450
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          452..485
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          486..516
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          518..548
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(551,553,557..558,561..563,565..566,570,573,582,584,
                     586,590..591,594..596,598..599,603,606,615,617,619,
                     623..624,627..629,631..632,636,639)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          551..582
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          584..615
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          589..670
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          617..647
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          828..930
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1153..1285
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1317..1400
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <1714..1965
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          <4328..4588
                     /region_name="YbbR"
                     /note="Cyclic di-AMP synthase regulator CdaR, YbbR domain
                     [Signal transduction mechanisms]; COG4856"
                     /db_xref="CDD:443884"
     Region          <6993..7306
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..8565
                     /gene="LOC144114148"
                     /coded_by="XM_077647691.1:635..26332"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 maaqenhdgv vrfllangan qslatedgft plavalqqgh dkvvavllen dargkvrlpa
       61 lhiaskkddc kaaalllhse hnpdvtsksg ftplhiaahy gnsniaslll dkgadvnfpa
      121 khqitplhva akwgksnmvk lllekgakid astrdgltpl hcaarsghdq vveqllekna
      181 pitaktkngl aplhmasqgd hvdsarilly hkapvddvtv dyltalhvaa hcghvgvakl
      241 lldrradpna ralngftplh iackknrikv velllkhgas ieattesglt plhvasfmgc
      301 mniviyliqh ganpdiptvr getplhlaar anqtdiiril lrngahvdak arelqtalhi
      361 asrlgnadmv glllqhgaav daptkdaytp lhvaaregqd evaallldhg aalaaptkkg
      421 ftplhlaaky gnlkvaqlll qkdapvdaqg kngvtplhva ahydhvnval lllekgasph
      481 saarngytpl hvaarkdqmd iasslleyga kpgaesragf tplhlaaqeg hadlaallve
      541 hgadcdakak ngltpmhlca qedrvevati lakhgasldp ttkagytplh vachfgqtnm
      601 irfllrqgan vnattshgyt plhqaaqqgh tliinllleh raapnavtnq gqtalaiaqr
      661 lgyisvvetl kvvtetivtt ttttvteeky rvvapetmhe tfmsdsedea aednmlgdqs
      721 fryltademk slgddslpid vtrderites ihitrepghp apltqeeerl sptqahttea
      781 ifvgnyapdn vdlsrtpiha gsllswdgds cssayvlspt krmwrerflv sflvdargga
      841 mrgcrhsgvr viipprkaqm pmritcrylr keklphpppl legeacasri levgpagakf
      901 lgpvilevph faslrgkere isilrsdnge twkehtleas eeavqevlne sfegeelsal
      961 edlqtnrivr ilttdfpqyf avvsrtrqev havgpeggll sstvvpqvqa ifpegaltkk
     1021 irvglqaqpi paelvtkllg hrvavspivt veprrrkfhk pitltipvpq aatkgminqy
     1081 agdaptlrll csitggtnka qwedvtgstp ltfvndcvsf tttvsarfwl mdcrqvseat
     1141 kfatelyaea mhvpfmakfv vfakrqdpme aqlrvfcmtd dkedktlesq ehftevaksr
     1201 dvevlekksq ylefggnlvp vtksgeqlql qfqafrenrl pfavrvkdph qeplgrlafm
     1261 rqpraarate ppqaplcnln ialpeyeasq nlselvtlek kygfveetgl akpelihrad
     1321 lrlsdiarel gsdwpalaaq ldvpeqdvas irtecpsdla qqallmlrlw mkraggratg
     1381 nnlekglrmi nredivnkcm fnvelvtddv ekavakvhld qsgfdtfree lgtpkdtslk
     1441 rdasldvsyd eqdlmkeaes aeetssetgs vhekhtgsea kgtskaipgv rkgspisaai
     1501 grivrgsske kaaprmavee tfeeepvlki dsaevtqqaa peagptappr dskkkskkek
     1561 spsplgpdys lprmrdyhtp erdftsttdt livssikedv daskelatdg kvkkekkkek
     1621 kekspsppgp dytlprlrdt ytppsdvtaa gyaastlpgq gitleqgivk eplerksker
     1681 slspgvvktg engslgadps verqvkqlsq gpedtiyekv ervvketpgk ivemvhikrq
     1741 rsskspegrg spkdknkglp ssevvapdis gesrsvpset igaekkqkkh ketsptkqgk
     1801 keakevseav dkdaelsasd gklpdsgfls kmaklpkkmf prsgkasasd eepsslsess
     1861 pvakkkagkl pksatlpkak psktplyeik svdvevdtsk ltskgvkgvp akdgeglets
     1921 rdkepdsrff skmaripkkm fprssktsss edepssasen lpsskqrakk ekeisskkke
     1981 kvvkvkpdsk klstsfevev drdtlpgagv dinakkpglq eslesesrkk tdsmqisfpq
     2041 svkavgskge pmeysldieg mkslqkepql avvgefpgkp spdielslts ketapefsdk
     2101 arghieigtp ehgksskggf mtkmaklprk mfphgfkgdv sveepsveae svhvsvpeaq
     2161 vklgtkdlkk kgpdmpamel taslpvgaeg svemsapdlp vsadgglisk vtqfpkmfsh
     2221 dsqdgvtier ppvvgegidv esslpdvqlk vkgkafekpe tkfdvtlper kvtpvlpdga
     2281 eghievnlde mgksyddgfm skmaklpkkm fshgledeia vqepsitqhd ihvdipklkd
     2341 eleiratlpe kpqvdfdvai pgkqvsldlp egveskievs apdvgkptdg gfmskvvqlp
     2401 kkmfphgskg tvpleeapea vgdihakvpe ahlkvglkgl ekpdfegdia vpgkkgavsl
     2461 pegtegkiev stpetgkpsd kgfmskmakl pkkmfphgsk gsvsveevpe vdrsvkvkvp
     2521 qgkipeahlk vdikdpekhk dvdialseek vdidlpegid knievtmpev skpseggfis
     2581 kmakfpkkmf phgakgsvsv eeapgidvdv kmpetrismd vkglekpkge vdislpkekv
     2641 didvpkgfdg klemsapevg kpseggfmsk makfpkkmfp hgakgsvsve eapgidvdvk
     2701 vpeahismdv kglekprgev dislpkekvd idvpkgfdgk lemsapevgk pseggfmskm
     2761 akfpkkmfph gakgslsvee tpgidvdvkm peadlsmdvk glekpkaeld iampkgkidi
     2821 dmpegvdgkl easapdigkp sdggfiskmt kfpkkmfphg akgtvsveap elgdvqvnmp
     2881 eahlgvdikg pekpkgeldv tlpkgkvdid lpegiegkve isapeigkpd ggfmskmakl
     2941 pkkmfphgak ggvsvegspe lgdvevkmpe ahlgvdikgp ekpkgeldvt lpkgkvdidl
     3001 pegiegkvei sapeigkpdg gfmskmaklp kkmfphgakg gvsvegspel gdvevkmpea
     3061 hlgvdikgpe kpkgeldvtl pkgkvdidlp egiegkveis apeigkpdgg fmskmaklpk
     3121 kmfphgakgg vsvegspelg dvevkmpeah lgvdikgpek pkgeldvtlp kgkvdidlpe
     3181 giegkveisa peigkpdggf mskmaklpkk mfshgakggv svegspelgd vqvkmpeahl
     3241 gvdikgpekp kgeldvtlpk gkvdidlpeg iegkveissp eigkpdggfm skmaklpkkm
     3301 fphgakggvs vegapelgdv evkmpeahlg vdikgpekpk geldvtlpkg kvdidlpegi
     3361 egkveisape igkpdggfms kmaklpkkmf phgakggvsv egapelgdve vkmpeahlgv
     3421 dikgpekpkg eldvtlpkgk vdidlpegfe gkveisapei gkpdggfmsk maklpkkmfp
     3481 hgtkggvsve gapelgdvev kmpeahlgvd ikgpekpkge ldvtlpkgkv didlpegieg
     3541 kveisspeig kpdggfmskm aklpkkmfph gakggvsveg apelgdvevk mpeahlgvdi
     3601 kgpekpkgel dvtlpkgkvd idlpegiegk veisapeigk pdggfmskma klpkkmfphg
     3661 akggvsvegs pelgdvevkm peahlgvdik gpekpkgeld vtlpkgkvdi dlpegiegkl
     3721 eisapeigkp dggfmskmak fpkkmfphga kggvsvegap elgdvqvkmp eahlgvdikg
     3781 pekpkgeldv tlpkgkvdid lpegiegkve isapeigkpd ggfmskmakf pkkmfphgak
     3841 ggvsvegape lgdvqvkmpe ahlgvdiegp ekpkgeldvt lpkgkvdidl pegiegkvei
     3901 sapeigkpdg gfmskmakfp kkmfphgtkg gvsvegapel gdvevkmpea hlgvdikgpe
     3961 kpkgeldvtl pkgkvdidlp egiegkveis apeigkpdgg fmskmaklpq kmfphgtkgg
     4021 vsvegapelg diqlkmpeah lgvdikgpek pkgeldamlp kgkvdidlpe giegkveisa
     4081 peigkpdggf mskmaklpkk mfshgakgsi svegspefgd vevkmpeahl ggdikgpekp
     4141 kgeldvalpk gkvdidipeg iegkveisap eigkpsdggf vskmaklpkk mfphgtkgsv
     4201 siegapevgd vqvkvpeaql kmdikgtgkp efegeitwtg kkvavelpeg iegtvemstp
     4261 emgrpsdggf mskvaklpkk vfshgskggv pfdeetgvag dievkmseaa lkvdvkypek
     4321 pesdfsalek kididvpegf kgkveistpe lgkpsdggfl skmsklpkkv lphgskgeis
     4381 agevsevsge vpvelpearl kvgvkgperp dsdvdisvsd kkvtldvtkg fegkvevstp
     4441 dlgkpsdkgf iskvtkfpkk mfphgskggv svgevsevtg dvhvelpdah lkvgvkgper
     4501 pdsdvdvsma vkkitvdvpe gfegklevst pelarspdgs lvskaaklpk klvpsgensl
     4561 ssveepslsa hvdvpgsdtt fkvdaklpam pvtdvdisls akessadipv gvagkveigt
     4621 pdagkpsdkg fmskmakipk kmfhhgpkss gstdehstva etvddkalgf evqlqkdvki
     4681 ptkpeadvsv sfhekevtvq lpegregtve lstpglvgeh vpdkssqgad vllpgkelpt
     4741 vegqeiiekp ggvievievt ahelskplss gllskvskip kkifprgsks kssdedspsp
     4801 gkrelketpg givevvevtk hdvaihpest hvkqvgtevl lepatsetkp sgaeglppth
     4861 itreikevpg givevvrvtk hivgklpedk gigepsvrtt daslpweael akdpsavikr
     4921 evketpeghv evvqvirrea sdvpfgsdfp gdlesdgtvs rriettvvdg kptvirrlvt
     4981 ttsdgirqvt ehieqgaeda gsaasslfak mgefakglss ddpsavttre vkqlpggave
     5041 vvsvtkrsss speteitsqe qlqsstsgsm hfaesavgkp meakttiirr eisgpetivr
     5101 tttsvvtsat apgsskpels advldkmfga mekprvddrl rdvltaeggy vakrevtpee
     5161 aellaqlfpe qstgvavypp aglvpeaaii peyrcmttae gahhdpeske kvllklgral
     5221 sqddldapsp tqgeaamtva glgeavdtsd qveepavekl elarreskry sqefeqdepl
     5281 pkamgfktkp seetvvsstq vkgvhdngtv khtvttvtkt tiiqeveepa gaprpgeset
     5341 wasdldtsqv svsslpeder teptlsssat tatadaeals gplartqvee kewtetdpds
     5401 gevrhiiskt thttvtskkp segaprgdtt sttvshsskd ghhgstpsap secdeaeslt
     5461 ffqkkeffek lseqsppket ssarlgstps ldtaltrpav tlkprprses ltsqgeiiee
     5521 sivfaerlrl fqngaqaspl psasipqvdl vdpapkmeqk lqgmaeigds lqqvpsavle
     5581 glqsteeksd trllvhtsda dkeafdvrsv qvaedfgdkq fshmrvrmpa peetvsktsi
     5641 cssdlpgdkp splrdaykgs lsasrqessq agkpplpsal ggdsslclvh eattdltlsk
     5701 agppnqtnkd ittfdrqpvr eqsptlrssq sqmlasglqm givagsgilq egikaspqsa
     5761 iletfsssat emtglespei rglpseevha gsaeeakgid agrigavlaa apvailpvrd
     5821 iisgkqleml diaqvsdepn ifegcvelps qaesqtcpke ktimkteyad iqhklssafg
     5881 eslapvesks gvtqgvpkev lealdrssqe liaskldeds erdtnilsat qgsiphdvfp
     5941 dhiestldns aavpmrgays tpsstavclt perleiqatr evaeerqekp evlvaleayr
     6001 kstlrrsged sydrnleefi tvleeyeplp agqlsdwkra spafepsich nseitsklpp
     6061 ledkkeletv gsildpsenv sellgnhaqs qmlcedtgll tdelgrtagh yvistaesdr
     6121 geclptlseq apgkereecl nydsalgvrs tsglesslle esheersldl arsedeqpsl
     6181 vedsmnvpte ssvidyqvvg hsgvvkdaqt qpkdsdfdlh eisrtgdktd lplscgddgk
     6241 ksaefsktvs giaadmvikd tdsehlsqdm lhptastvlc egrsdvpyss dedqhkelsr
     6301 ykevelvipf lrgtsssled evedggsfss gdlqaekiae flttdpdtln kvvhfpqifq
     6361 dydtsahqep fvptetemnv ekhvgfvlde maacstpcvp lksdrqteie easrivesvv
     6421 hiasslvesl hevnhetaik edicdsalay aagsplerse vsetlsqagp pstiekdqre
     6481 spfgvkemyq vlkesrgsed riqysvtsfg dplfadegde dqerrrrgsp taeslddqaq
     6541 iekcshevmm qlldgfsqsd velaevrted eapdieddvp speagsheek svpssqdnsg
     6601 dlqpqlstev eigsqasgap aplseldyel cpdadqlrts gdeavatrvp pleperdeav
     6661 aesshapsaa adpaasagrn aggrerspll easppeetay cpeqdispaq eypeyltpdm
     6721 qvrvgvspte aqpakekpvr ysessvesss evgspcppsq ggtamafenv afagedvida
     6781 tsspieesss pagsrspyev vrdahaeeaa qtalveaeks lrhavqeevr arpqslvtee
     6841 sseqqehqiq psifneafdh speesapekk qlsegllgvg lltvsdttar tpesrrgdsd
     6901 edeeewadhv mskirgrpip ptppasprav qsavevqgep weapvveqer vnvesardms
     6961 aerdagvdvm gdrsatqpql qefrrtadgk rpqlehipse isdedlvere qspthitatc
     7021 rgdrkdsssd rnsspdsdgp kasgshrrsa sgvgaialea vhsqdsggne khshesrdkg
     7081 aqeddkitee pvdtmfviee eapadsnrse sfcddadera artagglsps lesppqkkdl
     7141 ddgsssgkpd aaspvvlrsk skrtdkisdk rssrdldtgh sgsetdhshy ysfeltsdsg
     7201 ktpgasrpts sefdvnilsg qasseyetca tsqdgevsgs yataaasshd tsfatarssl
     7261 sgssrssays vdsessghlg smevseaset ivasaheeld sdiesersks vlrepydgei
     7321 pesvirggve hpflpswqpa lkdssptlhg sagarpptae gvfspfqstv psepgrscvg
     7381 wpssslstae svrspskpev lngvsapleg qrrlrhnddf massheeeql svsessatqs
     7441 ehtwqtsvet mvdaveaqrf scdserhhrq stmqnsvslp fdeavvaeds hreadddrat
     7501 ppfqevngpv eveyvpeydd ltartlrpvr qqnapdgqte aqgdvallea sagdastsre
     7561 qheeenstel reegfyaetf qgdlsseeve saaneallld ksacsfyeyc hakdeyevcn
     7621 trpavesasl keaferpatp epvdeiddqe degnlaqgsp nlhgkdyeaf ghasaeesvp
     7681 csdkthiaqe lsendqykvf sdsgnqsslh detrsksvss sleramtddv rlytskcedy
     7741 ilqmhqtyky gvglpktsdg gylpeegmdv envkllesrl qegnggqqdf pydlsyqqey
     7801 ggdgrvehgd lythqeveee gyddersrtr grrrlspisd ergstpdydt lagrktfgkg
     7861 sekddastss lmeferleae masgtksssv gssdslsgrq hqgrsgagse qdsvsvnslt
     7921 eferlekeiv eaegvdarlr gevaakldei deghesqasd pghetavyde sgatlllcas
     7981 advneepdgt tddaqlgdie aaqgqevaws rterhflttr tesghsvesv drqssastat
     8041 qfdadslrer eiddsesnfg pdtpgwmqke cvdldslhds lhdsehhdsl qevpskdkds
     8101 lqlrepsada dslqedhnel qdslyetrdi dwdsfradet sglddtsrsw hqfpsqirtt
     8161 elsptlsrpa ssplvvpvvv eridtltwvd qdpakacvsd geslgsrqqe hprndalvss
     8221 tdslepsssa nthatyqcdt dsmmsslnsa eestmvtstd tlehetlcla sqgkslsleg
     8281 glfsggdvte eqntrtpleg gvsvddmeer frecllrsaa sptneeqvee rqtmdekgnl
     8341 ivtrtvkrhv tseprlhtqt fmgpdaeqrs refvasfaam epsaerdeye gfdaagntvr
     8401 vtqhvvvrpt iktvtftgpd arlqmreymr slaggqhssa entptsdsgv gacsslaspp
     8461 plmpdppaaa pssacpglqr lsggdqwmea esalddcspp vtatrvittr ttrtmgpdgr
     8521 eqlvttttee sggeepearl rrsmqgvlds fmaepptpph ldeee
//
LOCUS       XP_077503818            5680 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X26 [Amblyomma
            americanum].
ACCESSION   XP_077503818
VERSION     XP_077503818.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647692.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..5680
                     /product="uncharacterized protein LOC144114148 isoform
                     X26"
                     /calculated_mol_wt=615192
     Region          59..90
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          73..390
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(92,94,98..99,102..104,106..107,111,114,123,125,127,
                     131..132,135..137,139..140,144,147,156,158,160,164..165,
                     168..170,172..173,177,180)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          92..123
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          125..156
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          158..183
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          191..216
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          224..251
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(253,255,259..260,263..265,267..268,272,275,284,286,
                     288,292..293,296..298,300..301,305,308,317,319,321,
                     325..326,329..331,333..334,338,341)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          253..284
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          286..317
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          319..349
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          333..621
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          352..416
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          418..449
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(451,453,457..458,461..463,465..466,470,473,482,484,
                     486,490..491,494..496,498..499,503,506,515,517,519,
                     523..524,527..529,531..532,536,539)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          451..482
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          484..515
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          499..777
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          517..546
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          550..575
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          583..614
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          616..649
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          650..680
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          682..712
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(715,717,721..722,725..727,729..730,734,737,746,748,
                     750,754..755,758..760,762..763,767,770,779,781,783,
                     787..788,791..793,795..796,800,803)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          715..746
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          748..779
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          753..834
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          781..811
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          999..1101
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1324..1456
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1488..1571
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <4108..4421
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..5680
                     /gene="LOC144114148"
                     /coded_by="XM_077647692.1:866..17908"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 mgpgeptirq sreswigcli rllasqndqc dpsvylflrw ltllllravl sqmmppatdg
       61 nasflraara gnlekvldyl kgsidintsn anglnalhla akeghvnvvs ellkrganvn
      121 attkkgnsal hiaslagqee vvkllvqkqa nvnvqsqngf tplymaaqen hdgvvrflla
      181 nganqslate dgftplaval qqghdkvvav llendargkv rlpalhiask kddckaaall
      241 lhsehnpdvt sksgftplhi aahygnsnia sllldkgadv nfpakhqitp lhvaakwgks
      301 nmvklllekg akidastrdg ltplhcaars ghdqvveqll eknapitakt knglaplhma
      361 sqgdhvdsar illyhkapvd dvtvdyltal hvaahcghvg vakllldrra dpnaralngf
      421 tplhiackkn rikvvelllk hgasieatte sgltplhvas fmgcmniviy liqhganpdi
      481 ptvrgetplh laaranqtdi irillrngah vdakarelqt alhiasrlgn admvglllqh
      541 gaavdaptkd aytplhvaar egqdevaall ldhgaalaap tkkgftplhl aakygnlkva
      601 qlllqkdapv daqgkngvtp lhvaahydhv nvallllekg asphsaarng ytplhvaark
      661 dqmdiassll eygakpgaes ragftplhla aqeghadlaa llvehgadcd akakngltpm
      721 hlcaqedrve vatilakhga sldpttkagy tplhvachfg qtnmirfllr qganvnatts
      781 hgytplhqaa qqghtliinl llehraapna vtnqgqtala iaqrlgyisv vetlkvvtet
      841 ivttttttvt eekyrvvape tmhetfmsds edeaaednml gdqsfrylta demkslgdds
      901 lpidvtrder itesihitre pghpapltqe eerlsptqah tteaifvgny apdnvdlsrt
      961 pihagsllsw dgdscssayv lsptkrmwre ssklkwktfl vsflvdargg amrgcrhsgv
     1021 rviipprkaq mpmritcryl rkeklphppp llegeacasr ilevgpagak flgpvilevp
     1081 hfaslrgker eisilrsdng etwkehtlea seeavqevln esfegeelsa ledlqtnriv
     1141 rilttdfpqy favvsrtrqe vhavgpeggl lsstvvpqvq aifpegaltk kirvglqaqp
     1201 ipaelvtkll ghrvavspiv tveprrrkfh kpitltipvp qaatkgminq yagdaptlrl
     1261 lcsitggtnk aqwedvtgst pltfvndcvs ftttvsarfw lmdcrqvsea tkfatelyae
     1321 amhvpfmakf vvfakrqdpm eaqlrvfcmt ddkedktles qehftevaks rdvevlekks
     1381 qylefggnlv pvtksgeqlq lqfqafrenr lpfavrvkdp hqeplgrlaf mrqpraarat
     1441 eppqaplcnl nialpeyeas qnlselvtle kkygfveetg lakpelihra dlrlsdiare
     1501 lgsdwpalaa qldvpeqdva sirtecpsdl aqqallmlrl wmkraggrat gnnlekglrm
     1561 inredivnkc mfnvelvtdd vekavakvhl dqsgfdtfre elgtpkdtsl krdasldvsy
     1621 deqdlmkeae saeetssetg svhekhtgse akgtskaipg vrkgspisaa igrivrgssk
     1681 ekaaprmave etfeeepvlk idsaevtqqa apeagptapp rdskkkskke kspsplgpdy
     1741 slprmrdyht perdftsttd tlivssiked vdaskelatd gkvkkekkke kkekspsppg
     1801 pdytlprlrd tytppsdvta agyaastlpg qvgehvpdks sqgadvllpg kelptvegqe
     1861 iiekpggvie vievtahels kplssgllsk vskipkkifp rgskskssde dspspgkrel
     1921 ketpggivev vevtkhdvai hpesthvkqv gtevllepat setkpsgaeg lppthitrei
     1981 kevpggivev vrvtkhivgk lpedkgigep svrttdaslp weaelakdps avikrevket
     2041 peghvevvqv irreasdvpf gsdfpgdles dgtvsrriet tvvdgkptvi rrlvtttsdg
     2101 irqvtehieq gaedagsaas slfakmgefa kglssddpsa vttrevkqlp ggavevvsvt
     2161 krsssspete itsqeqlqss tsgsmhfaes avgkpmeakt tiirreisgp etivrtttsv
     2221 vtsatapgss kpelsadvld kmfgamekpr vddrlrdvlt aeggyvakre vtpeeaella
     2281 qlfpeqstgv avyppaglvp eaaiipeyrc mttaegahhd peskekvllk lgralsqddl
     2341 dapsptqgea amtvaglgea vdtsdqveep aveklelarr eskrysqefe qdeplpkamg
     2401 fktkpseetv vsstqvkgvh dngtvkhtvt tvtkttiiqe veepagaprp gesetwasdl
     2461 dtsqvsvssl pederteptl sssattatad aealsgplar tqveekewte tdpdsgevrh
     2521 iisktthttv tskkpsegap rgdttsttvs hsskdghhgs tpsapsecde aesltffqkk
     2581 effeklseqs ppketssarl gstpsldtal trpavtlkpr prsesltsqg eiieesivfa
     2641 erlrlfqnga qasplpsasi pqvdlvdpap kmeqklqgma eigdslqqvp savleglqst
     2701 eeksdtrllv htsdadkeaf dvrsvqvaed fgdkqfshmr vrmpapeetv sktsicssdl
     2761 pgdkpsplrd aykgslsasr qessqagkpp lpsalggdss lclvheattd ltlskagppn
     2821 qtnkdittfd rqpvreqspt lrssqsqmla sglqmgivag sgilqegika spqsailetf
     2881 sssatemtgl espeirglps eevhagsaee akgidagrig avlaaapvai lpvrdiisgk
     2941 qlemldiaqv sdepnifegc velpsqaesq tcpkektimk teyadiqhkl ssafgeslap
     3001 vesksgvtqg vpkevleald rssqeliask ldedserdtn ilsatqgsip hdvfpdhies
     3061 tldnsaavpm rgaystpsst avcltperle iqatrevaee rqekpevlva leayrkstlr
     3121 rsgedsydrn leefitvlee yeplpagqls dwkraspafe psichnseit sklppledkk
     3181 eletvgsild psenvsellg nhaqsqmlce dtglltdelg rtaghyvist aesdrgeclp
     3241 tlseqapgke reeclnydsa lgvrstsgle sslleeshee rsldlarsed eqpslvedsm
     3301 nvptessvid yqvvghsgvv kdaqtqpkds dfdlheisrt gdktdlplsc gddgkksaef
     3361 sktvsgiaad mvikdtdseh lsqdmlhpta stvlcegrsd vpyssdedqh kelsrykeve
     3421 lvipflrgts ssledevedg gsfssgdlqa ekiaeflttd pdtlnkvvhf pqifqdydts
     3481 ahqepfvpte temnvekhvg fvldemaacs tpcvplksdr qteieeasri vesvvhiass
     3541 lveslhevnh etaikedicd salayaagsp lersevsetl sqagppstie kdqrespfgv
     3601 kemyqvlkes rgsedriqys vtsfgdplfa degdedqerr rrgsptaesl ddqaqiekcs
     3661 hevmmqlldg fsqsdvelae vrtedeapdi eddvpspeag sheeksvpss qdnsgdlqpq
     3721 lsteveigsq asgapaplse ldyelcpdad qlrtsgdeav atrvpplepe rdeavaessh
     3781 apsaaadpaa sagrnaggre rsplleaspp eetaycpeqd ispaqeypey ltpdmqvrvg
     3841 vspteaqpak ekpvrysess vesssevgsp cppsqggtam afenvafage dvidatsspi
     3901 eessspagsr spyevvrdah aeeaaqtalv eaekslrhav qeevrarpqs lvteesseqq
     3961 ehqiqpsifn eafdhspees apekkqlseg llgvglltvs dttartpesr rgdsdedeee
     4021 wadhvmskir grpipptppa spravqsave vqgepweapv veqervnves ardmsaerda
     4081 gvdvmgdrsa tqpqlqefrr tadgkrpqle hipseisded lvereqspth itatcrgdrk
     4141 dsssdrnssp dsdgpkasgs hrrsasgvga ialeavhsqd sggnekhshe srdkgaqedd
     4201 kiteepvdtm fvieeeapad snrsesfcdd aderaartag glspslespp qkkdlddgss
     4261 sgkpdaaspv vlrskskrtd kisdkrssrd ldtghsgset dhshyysfel tsdsgktpga
     4321 srptssefdv nilsgqasse yetcatsqdg evsgsyataa asshdtsfat arsslsgssr
     4381 ssaysvdses sghlgsmevs easetivasa heeldsdies ersksvlrep ydgeipesvi
     4441 rggvehpflp swqpalkdss ptlhgsagar pptaegvfsp fqstvpsepg rscvgwpsss
     4501 lstaesvrsp skpevlngvs aplegqrrlr hnddfmassh eeeqlsvses satqsehtwq
     4561 tsvetmvdav eaqrfscdse rhhrqstmqn svslpfdeav vaedshread ddratppfqe
     4621 vngpveveyv peyddltart lrpvrqqnap dgqteaqgdv alleasagda stsreqheee
     4681 nstelreegf yaetfqgdls seevesaane allldksacs fyeychakde yevcntrpav
     4741 esaslkeafe rpatpepvde iddqedegnl aqgspnlhgk dyeafghasa eesvpcsdkt
     4801 hiaqelsend qykvfsdsgn qsslhdetrs ksvssslera mtddvrlyts kcedyilqmh
     4861 qtykygvglp ktsdggylpe egmdvenvkl lesrlqegng gqqdfpydls yqqeyggdgr
     4921 vehgdlythq eveeegydde rsrtrgrrrl spisdergst pdydtlagrk tfgkgsekdd
     4981 astsslmefe rleaemasgt ksssvgssds lsgrqhqgrs gagseqdsvs vnslteferl
     5041 ekeiveaegv darlrgevaa kldeideghe sqasdpghet avydesgatl llcasadvne
     5101 epdgttddaq lgdieaaqgq evawsrterh flttrtesgh svesvdrqss astatqfdad
     5161 slrereidds esnfgpdtpg wmqkecvdld slhdslhdse hhdslqevps kdkdslqlre
     5221 psadadslqe dhnelqdsly etrdidwdsf radetsgldd tsrswhqfps qirttelspt
     5281 lsrpassplv vpvvveridt ltwvdqdpak acvsdgeslg srqqehprnd alvsstdsle
     5341 psssanthat yqcdtdsmms slnsaeestm vtstdtlehe tlclasqgks lslegglfsg
     5401 gdvteeqntr tpleggvsvd dmeerfrecl lrsaasptne eqveerqtmd ekgnlivtrt
     5461 vkrhvtsepr lhtqtfmgpd aeqrsrefva sfaamepsae rdeyegfdaa gntvrvtqhv
     5521 vvrptiktvt ftgpdarlqm reymrslagg qhssaentpt sdsgvgacss lasppplmpd
     5581 ppaaapssac pglqrlsggd qwmeaesald dcsppvtatr vittrttrtm gpdgreqlvt
     5641 ttteesggee pearlrrsmq gvldsfmaep ptpphldeee
//
LOCUS       XP_077503819            5501 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X27 [Amblyomma
            americanum].
ACCESSION   XP_077503819
VERSION     XP_077503819.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647693.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..5501
                     /product="uncharacterized protein LOC144114148 isoform
                     X27"
                     /calculated_mol_wt=595962
     Region          59..90
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          73..390
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(92,94,98..99,102..104,106..107,111,114,123,125,127,
                     131..132,135..137,139..140,144,147,156,158,160,164..165,
                     168..170,172..173,177,180)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          92..123
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          125..156
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          158..183
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          191..216
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          224..251
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(253,255,259..260,263..265,267..268,272,275,284,286,
                     288,292..293,296..298,300..301,305,308,317,319,321,
                     325..326,329..331,333..334,338,341)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          253..284
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          286..317
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          319..349
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          333..621
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          352..416
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          418..449
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(451,453,457..458,461..463,465..466,470,473,482,484,
                     486,490..491,494..496,498..499,503,506,515,517,519,
                     523..524,527..529,531..532,536,539)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          451..482
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          484..515
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          499..777
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          517..546
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          550..575
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          583..614
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          616..649
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          650..680
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          682..712
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(715,717,721..722,725..727,729..730,734,737,746,748,
                     750,754..755,758..760,762..763,767,770,779,781,783,
                     787..788,791..793,795..796,800,803)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          715..746
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          748..779
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          753..834
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          781..811
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          999..1101
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1324..1456
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1488..1571
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <3929..4242
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..5501
                     /gene="LOC144114148"
                     /coded_by="XM_077647693.1:864..17369"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 mgpgeptirq sreswigcli rllasqndqc dpsvylflrw ltllllravl sqmmppatdg
       61 nasflraara gnlekvldyl kgsidintsn anglnalhla akeghvnvvs ellkrganvn
      121 attkkgnsal hiaslagqee vvkllvqkqa nvnvqsqngf tplymaaqen hdgvvrflla
      181 nganqslate dgftplaval qqghdkvvav llendargkv rlpalhiask kddckaaall
      241 lhsehnpdvt sksgftplhi aahygnsnia sllldkgadv nfpakhqitp lhvaakwgks
      301 nmvklllekg akidastrdg ltplhcaars ghdqvveqll eknapitakt knglaplhma
      361 sqgdhvdsar illyhkapvd dvtvdyltal hvaahcghvg vakllldrra dpnaralngf
      421 tplhiackkn rikvvelllk hgasieatte sgltplhvas fmgcmniviy liqhganpdi
      481 ptvrgetplh laaranqtdi irillrngah vdakarelqt alhiasrlgn admvglllqh
      541 gaavdaptkd aytplhvaar egqdevaall ldhgaalaap tkkgftplhl aakygnlkva
      601 qlllqkdapv daqgkngvtp lhvaahydhv nvallllekg asphsaarng ytplhvaark
      661 dqmdiassll eygakpgaes ragftplhla aqeghadlaa llvehgadcd akakngltpm
      721 hlcaqedrve vatilakhga sldpttkagy tplhvachfg qtnmirfllr qganvnatts
      781 hgytplhqaa qqghtliinl llehraapna vtnqgqtala iaqrlgyisv vetlkvvtet
      841 ivttttttvt eekyrvvape tmhetfmsds edeaaednml gdqsfrylta demkslgdds
      901 lpidvtrder itesihitre pghpapltqe eerlsptqah tteaifvgny apdnvdlsrt
      961 pihagsllsw dgdscssayv lsptkrmwre ssklkwktfl vsflvdargg amrgcrhsgv
     1021 rviipprkaq mpmritcryl rkeklphppp llegeacasr ilevgpagak flgpvilevp
     1081 hfaslrgker eisilrsdng etwkehtlea seeavqevln esfegeelsa ledlqtnriv
     1141 rilttdfpqy favvsrtrqe vhavgpeggl lsstvvpqvq aifpegaltk kirvglqaqp
     1201 ipaelvtkll ghrvavspiv tveprrrkfh kpitltipvp qaatkgminq yagdaptlrl
     1261 lcsitggtnk aqwedvtgst pltfvndcvs ftttvsarfw lmdcrqvsea tkfatelyae
     1321 amhvpfmakf vvfakrqdpm eaqlrvfcmt ddkedktles qehftevaks rdvevlekks
     1381 qylefggnlv pvtksgeqlq lqfqafrenr lpfavrvkdp hqeplgrlaf mrqpraarat
     1441 eppqaplcnl nialpeyeas qnlselvtle kkygfveetg lakpelihra dlrlsdiare
     1501 lgsdwpalaa qldvpeqdva sirtecpsdl aqqallmlrl wmkraggrat gnnlekglrm
     1561 inredivnkc mfnvelvtdd vekavakvhl dqsgfdtfre elgtpkdtsl krdasldvsy
     1621 deqdlmkeae saeetssetg svhekhtgse akvgehvpdk ssqgadvllp gkelptvegq
     1681 eiiekpggvi evievtahel skplssglls kvskipkkif prgskskssd edspspgkre
     1741 lketpggive vvevtkhdva ihpesthvkq vgtevllepa tsetkpsgae glppthitre
     1801 ikevpggive vvrvtkhivg klpedkgige psvrttdasl pweaelakdp savikrevke
     1861 tpeghvevvq virreasdvp fgsdfpgdle sdgtvsrrie ttvvdgkptv irrlvtttsd
     1921 girqvtehie qgaedagsaa sslfakmgef akglssddps avttrevkql pggavevvsv
     1981 tkrsssspet eitsqeqlqs stsgsmhfae savgkpmeak ttiirreisg petivrttts
     2041 vvtsatapgs skpelsadvl dkmfgamekp rvddrlrdvl taeggyvakr evtpeeaell
     2101 aqlfpeqstg vavyppaglv peaaiipeyr cmttaegahh dpeskekvll klgralsqdd
     2161 ldapsptqge aamtvaglge avdtsdqvee paveklelar reskrysqef eqdeplpkam
     2221 gfktkpseet vvsstqvkgv hdngtvkhtv ttvtkttiiq eveepagapr pgesetwasd
     2281 ldtsqvsvss lpedertept lsssattata daealsgpla rtqveekewt etdpdsgevr
     2341 hiisktthtt vtskkpsega prgdttsttv shsskdghhg stpsapsecd eaesltffqk
     2401 keffeklseq sppketssar lgstpsldta ltrpavtlkp rprsesltsq geiieesivf
     2461 aerlrlfqng aqasplpsas ipqvdlvdpa pkmeqklqgm aeigdslqqv psavleglqs
     2521 teeksdtrll vhtsdadkea fdvrsvqvae dfgdkqfshm rvrmpapeet vsktsicssd
     2581 lpgdkpsplr daykgslsas rqessqagkp plpsalggds slclvheatt dltlskagpp
     2641 nqtnkdittf drqpvreqsp tlrssqsqml asglqmgiva gsgilqegik aspqsailet
     2701 fsssatemtg lespeirglp seevhagsae eakgidagri gavlaaapva ilpvrdiisg
     2761 kqlemldiaq vsdepnifeg cvelpsqaes qtcpkektim kteyadiqhk lssafgesla
     2821 pvesksgvtq gvpkevleal drssqelias kldedserdt nilsatqgsi phdvfpdhie
     2881 stldnsaavp mrgaystpss tavcltperl eiqatrevae erqekpevlv aleayrkstl
     2941 rrsgedsydr nleefitvle eyeplpagql sdwkraspaf epsichnsei tsklppledk
     3001 keletvgsil dpsenvsell gnhaqsqmlc edtglltdel grtaghyvis taesdrgecl
     3061 ptlseqapgk ereeclnyds algvrstsgl esslleeshe ersldlarse deqpslveds
     3121 mnvptessvi dyqvvghsgv vkdaqtqpkd sdfdlheisr tgdktdlpls cgddgkksae
     3181 fsktvsgiaa dmvikdtdse hlsqdmlhpt astvlcegrs dvpyssdedq hkelsrykev
     3241 elvipflrgt sssledeved ggsfssgdlq aekiaefltt dpdtlnkvvh fpqifqdydt
     3301 sahqepfvpt etemnvekhv gfvldemaac stpcvplksd rqteieeasr ivesvvhias
     3361 slveslhevn hetaikedic dsalayaags plersevset lsqagppsti ekdqrespfg
     3421 vkemyqvlke srgsedriqy svtsfgdplf adegdedqer rrrgsptaes lddqaqiekc
     3481 shevmmqlld gfsqsdvela evrtedeapd ieddvpspea gsheeksvps sqdnsgdlqp
     3541 qlsteveigs qasgapapls eldyelcpda dqlrtsgdea vatrvpplep erdeavaess
     3601 hapsaaadpa asagrnaggr ersplleasp peetaycpeq dispaqeype yltpdmqvrv
     3661 gvspteaqpa kekpvryses svesssevgs pcppsqggta mafenvafag edvidatssp
     3721 ieessspags rspyevvrda haeeaaqtal veaekslrha vqeevrarpq slvteesseq
     3781 qehqiqpsif neafdhspee sapekkqlse gllgvglltv sdttartpes rrgdsdedee
     3841 ewadhvmski rgrpipptpp aspravqsav evqgepweap vveqervnve sardmsaerd
     3901 agvdvmgdrs atqpqlqefr rtadgkrpql ehipseisde dlvereqspt hitatcrgdr
     3961 kdsssdrnss pdsdgpkasg shrrsasgvg aialeavhsq dsggnekhsh esrdkgaqed
     4021 dkiteepvdt mfvieeeapa dsnrsesfcd daderaarta gglspslesp pqkkdlddgs
     4081 ssgkpdaasp vvlrskskrt dkisdkrssr dldtghsgse tdhshyysfe ltsdsgktpg
     4141 asrptssefd vnilsgqass eyetcatsqd gevsgsyata aasshdtsfa tarsslsgss
     4201 rssaysvdse ssghlgsmev seasetivas aheeldsdie sersksvlre pydgeipesv
     4261 irggvehpfl pswqpalkds sptlhgsaga rpptaegvfs pfqstvpsep grscvgwpss
     4321 slstaesvrs pskpevlngv saplegqrrl rhnddfmass heeeqlsvse ssatqsehtw
     4381 qtsvetmvda veaqrfscds erhhrqstmq nsvslpfdea vvaedshrea dddratppfq
     4441 evngpvevey vpeyddltar tlrpvrqqna pdgqteaqgd valleasagd astsreqhee
     4501 enstelreeg fyaetfqgdl sseevesaan eallldksac sfyeychakd eyevcntrpa
     4561 vesaslkeaf erpatpepvd eiddqedegn laqgspnlhg kdyeafghas aeesvpcsdk
     4621 thiaqelsen dqykvfsdsg nqsslhdetr sksvsssler amtddvrlyt skcedyilqm
     4681 hqtykygvgl pktsdggylp eegmdvenvk llesrlqegn ggqqdfpydl syqqeyggdg
     4741 rvehgdlyth qeveeegydd ersrtrgrrr lspisdergs tpdydtlagr ktfgkgsekd
     4801 dastsslmef erleaemasg tksssvgssd slsgrqhqgr sgagseqdsv svnsltefer
     4861 lekeiveaeg vdarlrgeva akldeidegh esqasdpghe tavydesgat lllcasadvn
     4921 eepdgttdda qlgdieaaqg qevawsrter hflttrtesg hsvesvdrqs sastatqfda
     4981 dslrereidd sesnfgpdtp gwmqkecvdl dslhdslhds ehhdslqevp skdkdslqlr
     5041 epsadadslq edhnelqdsl yetrdidwds fradetsgld dtsrswhqfp sqirttelsp
     5101 tlsrpasspl vvpvvverid tltwvdqdpa kacvsdgesl gsrqqehprn dalvsstdsl
     5161 epsssantha tyqcdtdsmm sslnsaeest mvtstdtleh etlclasqgk slslegglfs
     5221 ggdvteeqnt rtpleggvsv ddmeerfrec llrsaasptn eeqveerqtm dekgnlivtr
     5281 tvkrhvtsep rlhtqtfmgp daeqrsrefv asfaamepsa erdeyegfda agntvrvtqh
     5341 vvvrptiktv tftgpdarlq mreymrslag gqhssaentp tsdsgvgacs slasppplmp
     5401 dppaaapssa cpglqrlsgg dqwmeaesal ddcsppvtat rvittrttrt mgpdgreqlv
     5461 tttteesgge epearlrrsm qgvldsfmae pptpphldee e
//
LOCUS       XP_077503820            5500 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X28 [Amblyomma
            americanum].
ACCESSION   XP_077503820
VERSION     XP_077503820.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647694.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..5500
                     /product="uncharacterized protein LOC144114148 isoform
                     X28"
                     /calculated_mol_wt=595833
     Region          59..90
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          73..390
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(92,94,98..99,102..104,106..107,111,114,123,125,127,
                     131..132,135..137,139..140,144,147,156,158,160,164..165,
                     168..170,172..173,177,180)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          92..123
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          125..156
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          158..183
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          191..216
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          224..251
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(253,255,259..260,263..265,267..268,272,275,284,286,
                     288,292..293,296..298,300..301,305,308,317,319,321,
                     325..326,329..331,333..334,338,341)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          253..284
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          286..317
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          319..349
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          333..621
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          352..416
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          418..449
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(451,453,457..458,461..463,465..466,470,473,482,484,
                     486,490..491,494..496,498..499,503,506,515,517,519,
                     523..524,527..529,531..532,536,539)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          451..482
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          484..515
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          499..777
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          517..546
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          550..575
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          583..614
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          616..649
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          650..680
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          682..712
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(715,717,721..722,725..727,729..730,734,737,746,748,
                     750,754..755,758..760,762..763,767,770,779,781,783,
                     787..788,791..793,795..796,800,803)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          715..746
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          748..779
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          753..834
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          781..811
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          999..1101
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1324..1456
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1488..1571
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <3928..4241
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..5500
                     /gene="LOC144114148"
                     /coded_by="XM_077647694.1:863..17365"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 mgpgeptirq sreswigcli rllasqndqc dpsvylflrw ltllllravl sqmmppatdg
       61 nasflraara gnlekvldyl kgsidintsn anglnalhla akeghvnvvs ellkrganvn
      121 attkkgnsal hiaslagqee vvkllvqkqa nvnvqsqngf tplymaaqen hdgvvrflla
      181 nganqslate dgftplaval qqghdkvvav llendargkv rlpalhiask kddckaaall
      241 lhsehnpdvt sksgftplhi aahygnsnia sllldkgadv nfpakhqitp lhvaakwgks
      301 nmvklllekg akidastrdg ltplhcaars ghdqvveqll eknapitakt knglaplhma
      361 sqgdhvdsar illyhkapvd dvtvdyltal hvaahcghvg vakllldrra dpnaralngf
      421 tplhiackkn rikvvelllk hgasieatte sgltplhvas fmgcmniviy liqhganpdi
      481 ptvrgetplh laaranqtdi irillrngah vdakarelqt alhiasrlgn admvglllqh
      541 gaavdaptkd aytplhvaar egqdevaall ldhgaalaap tkkgftplhl aakygnlkva
      601 qlllqkdapv daqgkngvtp lhvaahydhv nvallllekg asphsaarng ytplhvaark
      661 dqmdiassll eygakpgaes ragftplhla aqeghadlaa llvehgadcd akakngltpm
      721 hlcaqedrve vatilakhga sldpttkagy tplhvachfg qtnmirfllr qganvnatts
      781 hgytplhqaa qqghtliinl llehraapna vtnqgqtala iaqrlgyisv vetlkvvtet
      841 ivttttttvt eekyrvvape tmhetfmsds edeaaednml gdqsfrylta demkslgdds
      901 lpidvtrder itesihitre pghpapltqe eerlsptqah tteaifvgny apdnvdlsrt
      961 pihagsllsw dgdscssayv lsptkrmwre ssklkwktfl vsflvdargg amrgcrhsgv
     1021 rviipprkaq mpmritcryl rkeklphppp llegeacasr ilevgpagak flgpvilevp
     1081 hfaslrgker eisilrsdng etwkehtlea seeavqevln esfegeelsa ledlqtnriv
     1141 rilttdfpqy favvsrtrqe vhavgpeggl lsstvvpqvq aifpegaltk kirvglqaqp
     1201 ipaelvtkll ghrvavspiv tveprrrkfh kpitltipvp qaatkgminq yagdaptlrl
     1261 lcsitggtnk aqwedvtgst pltfvndcvs ftttvsarfw lmdcrqvsea tkfatelyae
     1321 amhvpfmakf vvfakrqdpm eaqlrvfcmt ddkedktles qehftevaks rdvevlekks
     1381 qylefggnlv pvtksgeqlq lqfqafrenr lpfavrvkdp hqeplgrlaf mrqpraarat
     1441 eppqaplcnl nialpeyeas qnlselvtle kkygfveetg lakpelihra dlrlsdiare
     1501 lgsdwpalaa qldvpeqdva sirtecpsdl aqqallmlrl wmkraggrat gnnlekglrm
     1561 inredivnkc mfnvelvtdd vekavakvhl dqsgfdtfre elgtpkdtsl krdasldvsy
     1621 deqdlmkaes aeetssetgs vhekhtgsea kvgehvpdks sqgadvllpg kelptvegqe
     1681 iiekpggvie vievtahels kplssgllsk vskipkkifp rgskskssde dspspgkrel
     1741 ketpggivev vevtkhdvai hpesthvkqv gtevllepat setkpsgaeg lppthitrei
     1801 kevpggivev vrvtkhivgk lpedkgigep svrttdaslp weaelakdps avikrevket
     1861 peghvevvqv irreasdvpf gsdfpgdles dgtvsrriet tvvdgkptvi rrlvtttsdg
     1921 irqvtehieq gaedagsaas slfakmgefa kglssddpsa vttrevkqlp ggavevvsvt
     1981 krsssspete itsqeqlqss tsgsmhfaes avgkpmeakt tiirreisgp etivrtttsv
     2041 vtsatapgss kpelsadvld kmfgamekpr vddrlrdvlt aeggyvakre vtpeeaella
     2101 qlfpeqstgv avyppaglvp eaaiipeyrc mttaegahhd peskekvllk lgralsqddl
     2161 dapsptqgea amtvaglgea vdtsdqveep aveklelarr eskrysqefe qdeplpkamg
     2221 fktkpseetv vsstqvkgvh dngtvkhtvt tvtkttiiqe veepagaprp gesetwasdl
     2281 dtsqvsvssl pederteptl sssattatad aealsgplar tqveekewte tdpdsgevrh
     2341 iisktthttv tskkpsegap rgdttsttvs hsskdghhgs tpsapsecde aesltffqkk
     2401 effeklseqs ppketssarl gstpsldtal trpavtlkpr prsesltsqg eiieesivfa
     2461 erlrlfqnga qasplpsasi pqvdlvdpap kmeqklqgma eigdslqqvp savleglqst
     2521 eeksdtrllv htsdadkeaf dvrsvqvaed fgdkqfshmr vrmpapeetv sktsicssdl
     2581 pgdkpsplrd aykgslsasr qessqagkpp lpsalggdss lclvheattd ltlskagppn
     2641 qtnkdittfd rqpvreqspt lrssqsqmla sglqmgivag sgilqegika spqsailetf
     2701 sssatemtgl espeirglps eevhagsaee akgidagrig avlaaapvai lpvrdiisgk
     2761 qlemldiaqv sdepnifegc velpsqaesq tcpkektimk teyadiqhkl ssafgeslap
     2821 vesksgvtqg vpkevleald rssqeliask ldedserdtn ilsatqgsip hdvfpdhies
     2881 tldnsaavpm rgaystpsst avcltperle iqatrevaee rqekpevlva leayrkstlr
     2941 rsgedsydrn leefitvlee yeplpagqls dwkraspafe psichnseit sklppledkk
     3001 eletvgsild psenvsellg nhaqsqmlce dtglltdelg rtaghyvist aesdrgeclp
     3061 tlseqapgke reeclnydsa lgvrstsgle sslleeshee rsldlarsed eqpslvedsm
     3121 nvptessvid yqvvghsgvv kdaqtqpkds dfdlheisrt gdktdlplsc gddgkksaef
     3181 sktvsgiaad mvikdtdseh lsqdmlhpta stvlcegrsd vpyssdedqh kelsrykeve
     3241 lvipflrgts ssledevedg gsfssgdlqa ekiaeflttd pdtlnkvvhf pqifqdydts
     3301 ahqepfvpte temnvekhvg fvldemaacs tpcvplksdr qteieeasri vesvvhiass
     3361 lveslhevnh etaikedicd salayaagsp lersevsetl sqagppstie kdqrespfgv
     3421 kemyqvlkes rgsedriqys vtsfgdplfa degdedqerr rrgsptaesl ddqaqiekcs
     3481 hevmmqlldg fsqsdvelae vrtedeapdi eddvpspeag sheeksvpss qdnsgdlqpq
     3541 lsteveigsq asgapaplse ldyelcpdad qlrtsgdeav atrvpplepe rdeavaessh
     3601 apsaaadpaa sagrnaggre rsplleaspp eetaycpeqd ispaqeypey ltpdmqvrvg
     3661 vspteaqpak ekpvrysess vesssevgsp cppsqggtam afenvafage dvidatsspi
     3721 eessspagsr spyevvrdah aeeaaqtalv eaekslrhav qeevrarpqs lvteesseqq
     3781 ehqiqpsifn eafdhspees apekkqlseg llgvglltvs dttartpesr rgdsdedeee
     3841 wadhvmskir grpipptppa spravqsave vqgepweapv veqervnves ardmsaerda
     3901 gvdvmgdrsa tqpqlqefrr tadgkrpqle hipseisded lvereqspth itatcrgdrk
     3961 dsssdrnssp dsdgpkasgs hrrsasgvga ialeavhsqd sggnekhshe srdkgaqedd
     4021 kiteepvdtm fvieeeapad snrsesfcdd aderaartag glspslespp qkkdlddgss
     4081 sgkpdaaspv vlrskskrtd kisdkrssrd ldtghsgset dhshyysfel tsdsgktpga
     4141 srptssefdv nilsgqasse yetcatsqdg evsgsyataa asshdtsfat arsslsgssr
     4201 ssaysvdses sghlgsmevs easetivasa heeldsdies ersksvlrep ydgeipesvi
     4261 rggvehpflp swqpalkdss ptlhgsagar pptaegvfsp fqstvpsepg rscvgwpsss
     4321 lstaesvrsp skpevlngvs aplegqrrlr hnddfmassh eeeqlsvses satqsehtwq
     4381 tsvetmvdav eaqrfscdse rhhrqstmqn svslpfdeav vaedshread ddratppfqe
     4441 vngpveveyv peyddltart lrpvrqqnap dgqteaqgdv alleasagda stsreqheee
     4501 nstelreegf yaetfqgdls seevesaane allldksacs fyeychakde yevcntrpav
     4561 esaslkeafe rpatpepvde iddqedegnl aqgspnlhgk dyeafghasa eesvpcsdkt
     4621 hiaqelsend qykvfsdsgn qsslhdetrs ksvssslera mtddvrlyts kcedyilqmh
     4681 qtykygvglp ktsdggylpe egmdvenvkl lesrlqegng gqqdfpydls yqqeyggdgr
     4741 vehgdlythq eveeegydde rsrtrgrrrl spisdergst pdydtlagrk tfgkgsekdd
     4801 astsslmefe rleaemasgt ksssvgssds lsgrqhqgrs gagseqdsvs vnslteferl
     4861 ekeiveaegv darlrgevaa kldeideghe sqasdpghet avydesgatl llcasadvne
     4921 epdgttddaq lgdieaaqgq evawsrterh flttrtesgh svesvdrqss astatqfdad
     4981 slrereidds esnfgpdtpg wmqkecvdld slhdslhdse hhdslqevps kdkdslqlre
     5041 psadadslqe dhnelqdsly etrdidwdsf radetsgldd tsrswhqfps qirttelspt
     5101 lsrpassplv vpvvveridt ltwvdqdpak acvsdgeslg srqqehprnd alvsstdsle
     5161 psssanthat yqcdtdsmms slnsaeestm vtstdtlehe tlclasqgks lslegglfsg
     5221 gdvteeqntr tpleggvsvd dmeerfrecl lrsaasptne eqveerqtmd ekgnlivtrt
     5281 vkrhvtsepr lhtqtfmgpd aeqrsrefva sfaamepsae rdeyegfdaa gntvrvtqhv
     5341 vvrptiktvt ftgpdarlqm reymrslagg qhssaentpt sdsgvgacss lasppplmpd
     5401 ppaaapssac pglqrlsggd qwmeaesald dcsppvtatr vittrttrtm gpdgreqlvt
     5461 ttteesggee pearlrrsmq gvldsfmaep ptpphldeee
//
LOCUS       XP_077503821            5500 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X29 [Amblyomma
            americanum].
ACCESSION   XP_077503821
VERSION     XP_077503821.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647695.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..5500
                     /product="uncharacterized protein LOC144114148 isoform
                     X29"
                     /calculated_mol_wt=595875
     Region          59..90
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          73..390
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(92,94,98..99,102..104,106..107,111,114,123,125,127,
                     131..132,135..137,139..140,144,147,156,158,160,164..165,
                     168..170,172..173,177,180)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          92..123
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          125..156
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          158..183
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          191..216
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          224..251
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(253,255,259..260,263..265,267..268,272,275,284,286,
                     288,292..293,296..298,300..301,305,308,317,319,321,
                     325..326,329..331,333..334,338,341)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          253..284
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          286..317
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          319..349
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          333..621
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          352..416
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          418..449
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(451,453,457..458,461..463,465..466,470,473,482,484,
                     486,490..491,494..496,498..499,503,506,515,517,519,
                     523..524,527..529,531..532,536,539)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          451..482
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          484..515
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          499..777
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          517..546
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          550..575
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          583..614
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          616..649
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          650..680
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          682..712
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(715,717,721..722,725..727,729..730,734,737,746,748,
                     750,754..755,758..760,762..763,767,770,779,781,783,
                     787..788,791..793,795..796,800,803)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          715..746
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          748..779
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          753..834
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          781..811
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          998..1100
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1323..1455
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1487..1570
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <3928..4241
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..5500
                     /gene="LOC144114148"
                     /coded_by="XM_077647695.1:864..17366"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 mgpgeptirq sreswigcli rllasqndqc dpsvylflrw ltllllravl sqmmppatdg
       61 nasflraara gnlekvldyl kgsidintsn anglnalhla akeghvnvvs ellkrganvn
      121 attkkgnsal hiaslagqee vvkllvqkqa nvnvqsqngf tplymaaqen hdgvvrflla
      181 nganqslate dgftplaval qqghdkvvav llendargkv rlpalhiask kddckaaall
      241 lhsehnpdvt sksgftplhi aahygnsnia sllldkgadv nfpakhqitp lhvaakwgks
      301 nmvklllekg akidastrdg ltplhcaars ghdqvveqll eknapitakt knglaplhma
      361 sqgdhvdsar illyhkapvd dvtvdyltal hvaahcghvg vakllldrra dpnaralngf
      421 tplhiackkn rikvvelllk hgasieatte sgltplhvas fmgcmniviy liqhganpdi
      481 ptvrgetplh laaranqtdi irillrngah vdakarelqt alhiasrlgn admvglllqh
      541 gaavdaptkd aytplhvaar egqdevaall ldhgaalaap tkkgftplhl aakygnlkva
      601 qlllqkdapv daqgkngvtp lhvaahydhv nvallllekg asphsaarng ytplhvaark
      661 dqmdiassll eygakpgaes ragftplhla aqeghadlaa llvehgadcd akakngltpm
      721 hlcaqedrve vatilakhga sldpttkagy tplhvachfg qtnmirfllr qganvnatts
      781 hgytplhqaa qqghtliinl llehraapna vtnqgqtala iaqrlgyisv vetlkvvtet
      841 ivttttttvt eekyrvvape tmhetfmsds edeaaednml gdqsfrylta demkslgdds
      901 lpidvtrder itesihitre pghpapltqe eerlsptqah tteaifvgny apdnvdlsrt
      961 pihagsllsw dgdscssayv lsptkrmwre sklkwktflv sflvdargga mrgcrhsgvr
     1021 viipprkaqm pmritcrylr keklphpppl legeacasri levgpagakf lgpvilevph
     1081 faslrgkere isilrsdnge twkehtleas eeavqevlne sfegeelsal edlqtnrivr
     1141 ilttdfpqyf avvsrtrqev havgpeggll sstvvpqvqa ifpegaltkk irvglqaqpi
     1201 paelvtkllg hrvavspivt veprrrkfhk pitltipvpq aatkgminqy agdaptlrll
     1261 csitggtnka qwedvtgstp ltfvndcvsf tttvsarfwl mdcrqvseat kfatelyaea
     1321 mhvpfmakfv vfakrqdpme aqlrvfcmtd dkedktlesq ehftevaksr dvevlekksq
     1381 ylefggnlvp vtksgeqlql qfqafrenrl pfavrvkdph qeplgrlafm rqpraarate
     1441 ppqaplcnln ialpeyeasq nlselvtlek kygfveetgl akpelihrad lrlsdiarel
     1501 gsdwpalaaq ldvpeqdvas irtecpsdla qqallmlrlw mkraggratg nnlekglrmi
     1561 nredivnkcm fnvelvtddv ekavakvhld qsgfdtfree lgtpkdtslk rdasldvsyd
     1621 eqdlmkeaes aeetssetgs vhekhtgsea kvgehvpdks sqgadvllpg kelptvegqe
     1681 iiekpggvie vievtahels kplssgllsk vskipkkifp rgskskssde dspspgkrel
     1741 ketpggivev vevtkhdvai hpesthvkqv gtevllepat setkpsgaeg lppthitrei
     1801 kevpggivev vrvtkhivgk lpedkgigep svrttdaslp weaelakdps avikrevket
     1861 peghvevvqv irreasdvpf gsdfpgdles dgtvsrriet tvvdgkptvi rrlvtttsdg
     1921 irqvtehieq gaedagsaas slfakmgefa kglssddpsa vttrevkqlp ggavevvsvt
     1981 krsssspete itsqeqlqss tsgsmhfaes avgkpmeakt tiirreisgp etivrtttsv
     2041 vtsatapgss kpelsadvld kmfgamekpr vddrlrdvlt aeggyvakre vtpeeaella
     2101 qlfpeqstgv avyppaglvp eaaiipeyrc mttaegahhd peskekvllk lgralsqddl
     2161 dapsptqgea amtvaglgea vdtsdqveep aveklelarr eskrysqefe qdeplpkamg
     2221 fktkpseetv vsstqvkgvh dngtvkhtvt tvtkttiiqe veepagaprp gesetwasdl
     2281 dtsqvsvssl pederteptl sssattatad aealsgplar tqveekewte tdpdsgevrh
     2341 iisktthttv tskkpsegap rgdttsttvs hsskdghhgs tpsapsecde aesltffqkk
     2401 effeklseqs ppketssarl gstpsldtal trpavtlkpr prsesltsqg eiieesivfa
     2461 erlrlfqnga qasplpsasi pqvdlvdpap kmeqklqgma eigdslqqvp savleglqst
     2521 eeksdtrllv htsdadkeaf dvrsvqvaed fgdkqfshmr vrmpapeetv sktsicssdl
     2581 pgdkpsplrd aykgslsasr qessqagkpp lpsalggdss lclvheattd ltlskagppn
     2641 qtnkdittfd rqpvreqspt lrssqsqmla sglqmgivag sgilqegika spqsailetf
     2701 sssatemtgl espeirglps eevhagsaee akgidagrig avlaaapvai lpvrdiisgk
     2761 qlemldiaqv sdepnifegc velpsqaesq tcpkektimk teyadiqhkl ssafgeslap
     2821 vesksgvtqg vpkevleald rssqeliask ldedserdtn ilsatqgsip hdvfpdhies
     2881 tldnsaavpm rgaystpsst avcltperle iqatrevaee rqekpevlva leayrkstlr
     2941 rsgedsydrn leefitvlee yeplpagqls dwkraspafe psichnseit sklppledkk
     3001 eletvgsild psenvsellg nhaqsqmlce dtglltdelg rtaghyvist aesdrgeclp
     3061 tlseqapgke reeclnydsa lgvrstsgle sslleeshee rsldlarsed eqpslvedsm
     3121 nvptessvid yqvvghsgvv kdaqtqpkds dfdlheisrt gdktdlplsc gddgkksaef
     3181 sktvsgiaad mvikdtdseh lsqdmlhpta stvlcegrsd vpyssdedqh kelsrykeve
     3241 lvipflrgts ssledevedg gsfssgdlqa ekiaeflttd pdtlnkvvhf pqifqdydts
     3301 ahqepfvpte temnvekhvg fvldemaacs tpcvplksdr qteieeasri vesvvhiass
     3361 lveslhevnh etaikedicd salayaagsp lersevsetl sqagppstie kdqrespfgv
     3421 kemyqvlkes rgsedriqys vtsfgdplfa degdedqerr rrgsptaesl ddqaqiekcs
     3481 hevmmqlldg fsqsdvelae vrtedeapdi eddvpspeag sheeksvpss qdnsgdlqpq
     3541 lsteveigsq asgapaplse ldyelcpdad qlrtsgdeav atrvpplepe rdeavaessh
     3601 apsaaadpaa sagrnaggre rsplleaspp eetaycpeqd ispaqeypey ltpdmqvrvg
     3661 vspteaqpak ekpvrysess vesssevgsp cppsqggtam afenvafage dvidatsspi
     3721 eessspagsr spyevvrdah aeeaaqtalv eaekslrhav qeevrarpqs lvteesseqq
     3781 ehqiqpsifn eafdhspees apekkqlseg llgvglltvs dttartpesr rgdsdedeee
     3841 wadhvmskir grpipptppa spravqsave vqgepweapv veqervnves ardmsaerda
     3901 gvdvmgdrsa tqpqlqefrr tadgkrpqle hipseisded lvereqspth itatcrgdrk
     3961 dsssdrnssp dsdgpkasgs hrrsasgvga ialeavhsqd sggnekhshe srdkgaqedd
     4021 kiteepvdtm fvieeeapad snrsesfcdd aderaartag glspslespp qkkdlddgss
     4081 sgkpdaaspv vlrskskrtd kisdkrssrd ldtghsgset dhshyysfel tsdsgktpga
     4141 srptssefdv nilsgqasse yetcatsqdg evsgsyataa asshdtsfat arsslsgssr
     4201 ssaysvdses sghlgsmevs easetivasa heeldsdies ersksvlrep ydgeipesvi
     4261 rggvehpflp swqpalkdss ptlhgsagar pptaegvfsp fqstvpsepg rscvgwpsss
     4321 lstaesvrsp skpevlngvs aplegqrrlr hnddfmassh eeeqlsvses satqsehtwq
     4381 tsvetmvdav eaqrfscdse rhhrqstmqn svslpfdeav vaedshread ddratppfqe
     4441 vngpveveyv peyddltart lrpvrqqnap dgqteaqgdv alleasagda stsreqheee
     4501 nstelreegf yaetfqgdls seevesaane allldksacs fyeychakde yevcntrpav
     4561 esaslkeafe rpatpepvde iddqedegnl aqgspnlhgk dyeafghasa eesvpcsdkt
     4621 hiaqelsend qykvfsdsgn qsslhdetrs ksvssslera mtddvrlyts kcedyilqmh
     4681 qtykygvglp ktsdggylpe egmdvenvkl lesrlqegng gqqdfpydls yqqeyggdgr
     4741 vehgdlythq eveeegydde rsrtrgrrrl spisdergst pdydtlagrk tfgkgsekdd
     4801 astsslmefe rleaemasgt ksssvgssds lsgrqhqgrs gagseqdsvs vnslteferl
     4861 ekeiveaegv darlrgevaa kldeideghe sqasdpghet avydesgatl llcasadvne
     4921 epdgttddaq lgdieaaqgq evawsrterh flttrtesgh svesvdrqss astatqfdad
     4981 slrereidds esnfgpdtpg wmqkecvdld slhdslhdse hhdslqevps kdkdslqlre
     5041 psadadslqe dhnelqdsly etrdidwdsf radetsgldd tsrswhqfps qirttelspt
     5101 lsrpassplv vpvvveridt ltwvdqdpak acvsdgeslg srqqehprnd alvsstdsle
     5161 psssanthat yqcdtdsmms slnsaeestm vtstdtlehe tlclasqgks lslegglfsg
     5221 gdvteeqntr tpleggvsvd dmeerfrecl lrsaasptne eqveerqtmd ekgnlivtrt
     5281 vkrhvtsepr lhtqtfmgpd aeqrsrefva sfaamepsae rdeyegfdaa gntvrvtqhv
     5341 vvrptiktvt ftgpdarlqm reymrslagg qhssaentpt sdsgvgacss lasppplmpd
     5401 ppaaapssac pglqrlsggd qwmeaesald dcsppvtatr vittrttrtm gpdgreqlvt
     5461 ttteesggee pearlrrsmq gvldsfmaep ptpphldeee
//
LOCUS       XP_077503822            4973 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X30 [Amblyomma
            americanum].
ACCESSION   XP_077503822
VERSION     XP_077503822.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647696.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..4973
                     /product="uncharacterized protein LOC144114148 isoform
                     X30"
                     /calculated_mol_wt=540024
     Region          59..90
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          73..390
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(92,94,98..99,102..104,106..107,111,114,123,125,127,
                     131..132,135..137,139..140,144,147,156,158,160,164..165,
                     168..170,172..173,177,180)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          92..123
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          125..156
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          158..183
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          191..216
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          224..251
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(253,255,259..260,263..265,267..268,272,275,284,286,
                     288,292..293,296..298,300..301,305,308,317,319,321,
                     325..326,329..331,333..334,338,341)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          253..284
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          286..317
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          319..349
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          333..621
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          352..416
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          418..449
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(451,453,457..458,461..463,465..466,470,473,482,484,
                     486,490..491,494..496,498..499,503,506,515,517,519,
                     523..524,527..529,531..532,536,539)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          451..482
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          484..515
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          499..777
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          517..546
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          550..575
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          583..614
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          616..649
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          650..680
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          682..712
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(715,717,721..722,725..727,729..730,734,737,746,748,
                     750,754..755,758..760,762..763,767,770,779,781,783,
                     787..788,791..793,795..796,800,803)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          715..746
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          748..779
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          753..834
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          781..811
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          999..1101
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1324..1456
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1488..1571
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <3438..3714
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..4973
                     /gene="LOC144114148"
                     /coded_by="XM_077647696.1:881..15802"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 mgpgeptirq sreswigcli rllasqndqc dpsvylflrw ltllllravl sqmmppatdg
       61 nasflraara gnlekvldyl kgsidintsn anglnalhla akeghvnvvs ellkrganvn
      121 attkkgnsal hiaslagqee vvkllvqkqa nvnvqsqngf tplymaaqen hdgvvrflla
      181 nganqslate dgftplaval qqghdkvvav llendargkv rlpalhiask kddckaaall
      241 lhsehnpdvt sksgftplhi aahygnsnia sllldkgadv nfpakhqitp lhvaakwgks
      301 nmvklllekg akidastrdg ltplhcaars ghdqvveqll eknapitakt knglaplhma
      361 sqgdhvdsar illyhkapvd dvtvdyltal hvaahcghvg vakllldrra dpnaralngf
      421 tplhiackkn rikvvelllk hgasieatte sgltplhvas fmgcmniviy liqhganpdi
      481 ptvrgetplh laaranqtdi irillrngah vdakarelqt alhiasrlgn admvglllqh
      541 gaavdaptkd aytplhvaar egqdevaall ldhgaalaap tkkgftplhl aakygnlkva
      601 qlllqkdapv daqgkngvtp lhvaahydhv nvallllekg asphsaarng ytplhvaark
      661 dqmdiassll eygakpgaes ragftplhla aqeghadlaa llvehgadcd akakngltpm
      721 hlcaqedrve vatilakhga sldpttkagy tplhvachfg qtnmirfllr qganvnatts
      781 hgytplhqaa qqghtliinl llehraapna vtnqgqtala iaqrlgyisv vetlkvvtet
      841 ivttttttvt eekyrvvape tmhetfmsds edeaaednml gdqsfrylta demkslgdds
      901 lpidvtrder itesihitre pghpapltqe eerlsptqah tteaifvgny apdnvdlsrt
      961 pihagsllsw dgdscssayv lsptkrmwre ssklkwktfl vsflvdargg amrgcrhsgv
     1021 rviipprkaq mpmritcryl rkeklphppp llegeacasr ilevgpagak flgpvilevp
     1081 hfaslrgker eisilrsdng etwkehtlea seeavqevln esfegeelsa ledlqtnriv
     1141 rilttdfpqy favvsrtrqe vhavgpeggl lsstvvpqvq aifpegaltk kirvglqaqp
     1201 ipaelvtkll ghrvavspiv tveprrrkfh kpitltipvp qaatkgminq yagdaptlrl
     1261 lcsitggtnk aqwedvtgst pltfvndcvs ftttvsarfw lmdcrqvsea tkfatelyae
     1321 amhvpfmakf vvfakrqdpm eaqlrvfcmt ddkedktles qehftevaks rdvevlekks
     1381 qylefggnlv pvtksgeqlq lqfqafrenr lpfavrvkdp hqeplgrlaf mrqpraarat
     1441 eppqaplcnl nialpeyeas qnlselvtle kkygfveetg lakpelihra dlrlsdiare
     1501 lgsdwpalaa qldvpeqdva sirtecpsdl aqqallmlrl wmkraggrat gnnlekglrm
     1561 inredivnkc mfnvelvtdd vekavakvhl dqsgfdtfre elgtpkdtsl krdasldvsy
     1621 deqdlmkeae saeetssetg svhekhtgse akavdtsdqv eepaveklel arreskrysq
     1681 efeqdeplpk amgfktkpse etvvsstqvk gvhdngtvkh tvttvtktti iqeveepaga
     1741 prpgesetwa sdldtsqvsv sslpederte ptlsssatta tadaealsgp lartqveeke
     1801 wtetdpdsge vrhiisktth ttvtskkpse gaprgdttst tvshsskdgh hgstpsapse
     1861 cdeaesltff qkkeffekls eqsppketss arlgstpsld taltrpavtl kprprseslt
     1921 sqgeiieesi vfaerlrlfq ngaqasplps asipqvdlvd papkmeqklq gmaeigdslq
     1981 qvpsavlegl qsteeksdtr llvhtsdadk eafdvrsvqv aedfgdkqfs hmrvrmpape
     2041 etvsktsics sdlpgdkpsp lrdaykgsls asrqessqag kpplpsalgg dsslclvhea
     2101 ttdltlskag ppnqtnkdit tfdrqpvreq sptlrssqsq mlasglqmgi vagsgilqeg
     2161 ikaspqsail etfsssatem tglespeirg lpseevhags aeeakgidag rigavlaaap
     2221 vailpvrdii sgkqlemldi aqvsdepnif egcvelpsqa esqtcpkekt imkteyadiq
     2281 hklssafges lapvesksgv tqgvpkevle aldrssqeli askldedser dtnilsatqg
     2341 siphdvfpdh iestldnsaa vpmrgaystp sstavcltpe rleiqatrev aeerqekpev
     2401 lvaleayrks tlrrsgedsy drnleefitv leeyeplpag qlsdwkrasp afepsichns
     2461 eitsklpple dkkeletvgs ildpsenvse llgnhaqsqm lcedtglltd elgrtaghyv
     2521 istaesdrge clptlseqap gkereeclny dsalgvrsts glessllees heersldlar
     2581 sedeqpslve dsmnvptess vidyqvvghs gvvkdaqtqp kdsdfdlhei srtgdktdlp
     2641 lscgddgkks aefsktvsgi aadmvikdtd sehlsqdmlh ptastvlceg rsdvpyssde
     2701 dqhkelsryk evelvipflr gtsssledev edggsfssgd lqaekiaefl ttdpdtlnkv
     2761 vhfpqifqdy dtsahqepfv ptetemnvek hvgfvldema acstpcvplk sdrqteieea
     2821 srivesvvhi asslveslhe vnhetaiked icdsalayaa gsplersevs etlsqagpps
     2881 tiekdqresp fgvkemyqvl kesrgsedri qysvtsfgdp lfadegdedq errrrgspta
     2941 eslddqaqie kcshevmmql ldgfsqsdve laevrtedea pdieddvpsp eagsheeksv
     3001 pssqdnsgdl qpqlstevei gsqasgapap lseldyelcp dadqlrtsgd eavatrvppl
     3061 eperdeavae sshapsaaad paasagrnag grerspllea sppeetaycp eqdispaqey
     3121 peyltpdmqv rvgvspteaq pakekpvrys essvesssev gspcppsqgg tamafenvaf
     3181 agedvidats spieessspa gsrspyevvr dahaeeaaqt alveaekslr havqeevrar
     3241 pqslvteess eqqehqiqps ifneafdhsp eesapekkql segllgvgll tvsdttartp
     3301 esrrgdsded eeewadhvms kirgrpippt ppaspravqs avevqgepwe apvveqervn
     3361 vesardmsae rdagvdvmgd rsatqpqlqe frrtadgkrp qlehipseis dedlvereqs
     3421 pthitatcrg drkdsssdrn sspdsdgpka sgshrrsasg vgaialeavh sqdsggnekh
     3481 shesrdkgaq eddkiteepv dtmfvieeea padsnrsesf cddaderaar tagglspsle
     3541 sppqkkdldd gsssgkpdaa spvvlrsksk rtdkisdkrs srdldtghsg setdhshyys
     3601 feltsdsgkt pgasrptsse fdvnilsgqa sseyetcats qdgevsgsya taaasshdts
     3661 fatarsslsg ssrssaysvd sessghlgsm evseasetiv asaheeldsd iesersksvl
     3721 repydgeipe svirggvehp flpswqpalk dssptlhgsa garpptaegv fspfqstvps
     3781 epgrscvgwp ssslstaesv rspskpevln gvsaplegqr rlrhnddfma ssheeeqlsv
     3841 sessatqseh twqtsvetmv daveaqrfsc dserhhrqst mqnsvslpfd eavvaedshr
     3901 eadddratpp fqevngpvev eyvpeyddlt artlrpvrqq napdgqteaq gdvalleasa
     3961 gdastsreqh eeenstelre egfyaetfqg dlsseevesa aneallldks acsfyeycha
     4021 kdeyevcntr pavesaslke aferpatpep vdeiddqede gnlaqgspnl hgkdyeafgh
     4081 asaeesvpcs dkthiaqels endqykvfsd sgnqsslhde trsksvsssl eramtddvrl
     4141 ytskcedyil qmhqtykygv glpktsdggy lpeegmdven vkllesrlqe gnggqqdfpy
     4201 dlsyqqeygg dgrvehgdly thqeveeegy ddersrtrgr rrlspisder gstpdydtla
     4261 grktfgkgse kddastsslm eferleaema sgtksssvgs sdslsgrqhq grsgagseqd
     4321 svsvnsltef erlekeivea egvdarlrge vaakldeide ghesqasdpg hetavydesg
     4381 atlllcasad vneepdgttd daqlgdieaa qgqevawsrt erhflttrte sghsvesvdr
     4441 qssastatqf dadslrerei ddsesnfgpd tpgwmqkecv dldslhdslh dsehhdslqe
     4501 vpskdkdslq lrepsadads lqedhnelqd slyetrdidw dsfradetsg lddtsrswhq
     4561 fpsqirttel sptlsrpass plvvpvvver idtltwvdqd pakacvsdge slgsrqqehp
     4621 rndalvsstd slepsssant hatyqcdtds mmsslnsaee stmvtstdtl ehetlclasq
     4681 gkslsleggl fsggdvteeq ntrtpleggv svddmeerfr ecllrsaasp tneeqveerq
     4741 tmdekgnliv trtvkrhvts eprlhtqtfm gpdaeqrsre fvasfaamep saerdeyegf
     4801 daagntvrvt qhvvvrptik tvtftgpdar lqmreymrsl aggqhssaen tptsdsgvga
     4861 csslaspppl mpdppaaaps sacpglqrls ggdqwmeaes alddcsppvt atrvittrtt
     4921 rtmgpdgreq lvtttteesg geepearlrr smqgvldsfm aepptpphld eee
//
LOCUS       XP_077503823            4972 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X31 [Amblyomma
            americanum].
ACCESSION   XP_077503823
VERSION     XP_077503823.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647697.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..4972
                     /product="uncharacterized protein LOC144114148 isoform
                     X31"
                     /calculated_mol_wt=539895
     Region          59..90
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          73..390
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(92,94,98..99,102..104,106..107,111,114,123,125,127,
                     131..132,135..137,139..140,144,147,156,158,160,164..165,
                     168..170,172..173,177,180)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          92..123
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          125..156
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          158..183
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          191..216
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          224..251
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(253,255,259..260,263..265,267..268,272,275,284,286,
                     288,292..293,296..298,300..301,305,308,317,319,321,
                     325..326,329..331,333..334,338,341)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          253..284
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          286..317
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          319..349
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          333..621
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          352..416
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          418..449
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(451,453,457..458,461..463,465..466,470,473,482,484,
                     486,490..491,494..496,498..499,503,506,515,517,519,
                     523..524,527..529,531..532,536,539)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          451..482
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          484..515
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          499..777
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          517..546
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          550..575
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          583..614
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          616..649
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          650..680
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          682..712
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(715,717,721..722,725..727,729..730,734,737,746,748,
                     750,754..755,758..760,762..763,767,770,779,781,783,
                     787..788,791..793,795..796,800,803)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          715..746
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          748..779
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          753..834
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          781..811
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          999..1101
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1324..1456
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1488..1571
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <3400..3713
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..4972
                     /gene="LOC144114148"
                     /coded_by="XM_077647697.1:880..15798"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 mgpgeptirq sreswigcli rllasqndqc dpsvylflrw ltllllravl sqmmppatdg
       61 nasflraara gnlekvldyl kgsidintsn anglnalhla akeghvnvvs ellkrganvn
      121 attkkgnsal hiaslagqee vvkllvqkqa nvnvqsqngf tplymaaqen hdgvvrflla
      181 nganqslate dgftplaval qqghdkvvav llendargkv rlpalhiask kddckaaall
      241 lhsehnpdvt sksgftplhi aahygnsnia sllldkgadv nfpakhqitp lhvaakwgks
      301 nmvklllekg akidastrdg ltplhcaars ghdqvveqll eknapitakt knglaplhma
      361 sqgdhvdsar illyhkapvd dvtvdyltal hvaahcghvg vakllldrra dpnaralngf
      421 tplhiackkn rikvvelllk hgasieatte sgltplhvas fmgcmniviy liqhganpdi
      481 ptvrgetplh laaranqtdi irillrngah vdakarelqt alhiasrlgn admvglllqh
      541 gaavdaptkd aytplhvaar egqdevaall ldhgaalaap tkkgftplhl aakygnlkva
      601 qlllqkdapv daqgkngvtp lhvaahydhv nvallllekg asphsaarng ytplhvaark
      661 dqmdiassll eygakpgaes ragftplhla aqeghadlaa llvehgadcd akakngltpm
      721 hlcaqedrve vatilakhga sldpttkagy tplhvachfg qtnmirfllr qganvnatts
      781 hgytplhqaa qqghtliinl llehraapna vtnqgqtala iaqrlgyisv vetlkvvtet
      841 ivttttttvt eekyrvvape tmhetfmsds edeaaednml gdqsfrylta demkslgdds
      901 lpidvtrder itesihitre pghpapltqe eerlsptqah tteaifvgny apdnvdlsrt
      961 pihagsllsw dgdscssayv lsptkrmwre ssklkwktfl vsflvdargg amrgcrhsgv
     1021 rviipprkaq mpmritcryl rkeklphppp llegeacasr ilevgpagak flgpvilevp
     1081 hfaslrgker eisilrsdng etwkehtlea seeavqevln esfegeelsa ledlqtnriv
     1141 rilttdfpqy favvsrtrqe vhavgpeggl lsstvvpqvq aifpegaltk kirvglqaqp
     1201 ipaelvtkll ghrvavspiv tveprrrkfh kpitltipvp qaatkgminq yagdaptlrl
     1261 lcsitggtnk aqwedvtgst pltfvndcvs ftttvsarfw lmdcrqvsea tkfatelyae
     1321 amhvpfmakf vvfakrqdpm eaqlrvfcmt ddkedktles qehftevaks rdvevlekks
     1381 qylefggnlv pvtksgeqlq lqfqafrenr lpfavrvkdp hqeplgrlaf mrqpraarat
     1441 eppqaplcnl nialpeyeas qnlselvtle kkygfveetg lakpelihra dlrlsdiare
     1501 lgsdwpalaa qldvpeqdva sirtecpsdl aqqallmlrl wmkraggrat gnnlekglrm
     1561 inredivnkc mfnvelvtdd vekavakvhl dqsgfdtfre elgtpkdtsl krdasldvsy
     1621 deqdlmkaes aeetssetgs vhekhtgsea kavdtsdqve epaveklela rreskrysqe
     1681 feqdeplpka mgfktkpsee tvvsstqvkg vhdngtvkht vttvtkttii qeveepagap
     1741 rpgesetwas dldtsqvsvs slpedertep tlsssattat adaealsgpl artqveekew
     1801 tetdpdsgev rhiiskttht tvtskkpseg aprgdttstt vshsskdghh gstpsapsec
     1861 deaesltffq kkeffeklse qsppketssa rlgstpsldt altrpavtlk prprseslts
     1921 qgeiieesiv faerlrlfqn gaqasplpsa sipqvdlvdp apkmeqklqg maeigdslqq
     1981 vpsavleglq steeksdtrl lvhtsdadke afdvrsvqva edfgdkqfsh mrvrmpapee
     2041 tvsktsicss dlpgdkpspl rdaykgslsa srqessqagk pplpsalggd sslclvheat
     2101 tdltlskagp pnqtnkditt fdrqpvreqs ptlrssqsqm lasglqmgiv agsgilqegi
     2161 kaspqsaile tfsssatemt glespeirgl pseevhagsa eeakgidagr igavlaaapv
     2221 ailpvrdiis gkqlemldia qvsdepnife gcvelpsqae sqtcpkekti mkteyadiqh
     2281 klssafgesl apvesksgvt qgvpkevlea ldrssqelia skldedserd tnilsatqgs
     2341 iphdvfpdhi estldnsaav pmrgaystps stavcltper leiqatreva eerqekpevl
     2401 valeayrkst lrrsgedsyd rnleefitvl eeyeplpagq lsdwkraspa fepsichnse
     2461 itsklppled kkeletvgsi ldpsenvsel lgnhaqsqml cedtglltde lgrtaghyvi
     2521 staesdrgec lptlseqapg kereeclnyd salgvrstsg lesslleesh eersldlars
     2581 edeqpslved smnvptessv idyqvvghsg vvkdaqtqpk dsdfdlheis rtgdktdlpl
     2641 scgddgkksa efsktvsgia admvikdtds ehlsqdmlhp tastvlcegr sdvpyssded
     2701 qhkelsryke velvipflrg tsssledeve dggsfssgdl qaekiaeflt tdpdtlnkvv
     2761 hfpqifqdyd tsahqepfvp tetemnvekh vgfvldemaa cstpcvplks drqteieeas
     2821 rivesvvhia sslveslhev nhetaikedi cdsalayaag splersevse tlsqagppst
     2881 iekdqrespf gvkemyqvlk esrgsedriq ysvtsfgdpl fadegdedqe rrrrgsptae
     2941 slddqaqiek cshevmmqll dgfsqsdvel aevrtedeap dieddvpspe agsheeksvp
     3001 ssqdnsgdlq pqlsteveig sqasgapapl seldyelcpd adqlrtsgde avatrvpple
     3061 perdeavaes shapsaaadp aasagrnagg rersplleas ppeetaycpe qdispaqeyp
     3121 eyltpdmqvr vgvspteaqp akekpvryse ssvesssevg spcppsqggt amafenvafa
     3181 gedvidatss pieessspag srspyevvrd ahaeeaaqta lveaekslrh avqeevrarp
     3241 qslvteesse qqehqiqpsi fneafdhspe esapekkqls egllgvgllt vsdttartpe
     3301 srrgdsdede eewadhvmsk irgrpipptp paspravqsa vevqgepwea pvveqervnv
     3361 esardmsaer dagvdvmgdr satqpqlqef rrtadgkrpq lehipseisd edlvereqsp
     3421 thitatcrgd rkdsssdrns spdsdgpkas gshrrsasgv gaialeavhs qdsggnekhs
     3481 hesrdkgaqe ddkiteepvd tmfvieeeap adsnrsesfc ddaderaart agglspsles
     3541 ppqkkdlddg sssgkpdaas pvvlrskskr tdkisdkrss rdldtghsgs etdhshyysf
     3601 eltsdsgktp gasrptssef dvnilsgqas seyetcatsq dgevsgsyat aaasshdtsf
     3661 atarsslsgs srssaysvds essghlgsme vseasetiva saheeldsdi esersksvlr
     3721 epydgeipes virggvehpf lpswqpalkd ssptlhgsag arpptaegvf spfqstvpse
     3781 pgrscvgwps sslstaesvr spskpevlng vsaplegqrr lrhnddfmas sheeeqlsvs
     3841 essatqseht wqtsvetmvd aveaqrfscd serhhrqstm qnsvslpfde avvaedshre
     3901 adddratppf qevngpveve yvpeyddlta rtlrpvrqqn apdgqteaqg dvalleasag
     3961 dastsreqhe eenstelree gfyaetfqgd lsseevesaa neallldksa csfyeychak
     4021 deyevcntrp avesaslkea ferpatpepv deiddqedeg nlaqgspnlh gkdyeafgha
     4081 saeesvpcsd kthiaqelse ndqykvfsds gnqsslhdet rsksvsssle ramtddvrly
     4141 tskcedyilq mhqtykygvg lpktsdggyl peegmdvenv kllesrlqeg nggqqdfpyd
     4201 lsyqqeyggd grvehgdlyt hqeveeegyd dersrtrgrr rlspisderg stpdydtlag
     4261 rktfgkgsek ddastsslme ferleaemas gtksssvgss dslsgrqhqg rsgagseqds
     4321 vsvnsltefe rlekeiveae gvdarlrgev aakldeideg hesqasdpgh etavydesga
     4381 tlllcasadv neepdgttdd aqlgdieaaq gqevawsrte rhflttrtes ghsvesvdrq
     4441 ssastatqfd adslrereid dsesnfgpdt pgwmqkecvd ldslhdslhd sehhdslqev
     4501 pskdkdslql repsadadsl qedhnelqds lyetrdidwd sfradetsgl ddtsrswhqf
     4561 psqirttels ptlsrpassp lvvpvvveri dtltwvdqdp akacvsdges lgsrqqehpr
     4621 ndalvsstds lepsssanth atyqcdtdsm msslnsaees tmvtstdtle hetlclasqg
     4681 kslslegglf sggdvteeqn trtpleggvs vddmeerfre cllrsaaspt neeqveerqt
     4741 mdekgnlivt rtvkrhvtse prlhtqtfmg pdaeqrsref vasfaameps aerdeyegfd
     4801 aagntvrvtq hvvvrptikt vtftgpdarl qmreymrsla ggqhssaent ptsdsgvgac
     4861 sslasppplm pdppaaapss acpglqrlsg gdqwmeaesa lddcsppvta trvittrttr
     4921 tmgpdgreql vtttteesgg eepearlrrs mqgvldsfma epptpphlde ee
//
LOCUS       XP_077503824            4972 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X32 [Amblyomma
            americanum].
ACCESSION   XP_077503824
VERSION     XP_077503824.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647698.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..4972
                     /product="uncharacterized protein LOC144114148 isoform
                     X32"
                     /calculated_mol_wt=539953
     Region          59..90
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          73..390
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(92,94,98..99,102..104,106..107,111,114,123,125,127,
                     131..132,135..137,139..140,144,147,156,158,160,164..165,
                     168..170,172..173,177,180)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          92..123
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          125..156
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          158..183
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          191..216
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          224..251
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(253,255,259..260,263..265,267..268,272,275,284,286,
                     288,292..293,296..298,300..301,305,308,317,319,321,
                     325..326,329..331,333..334,338,341)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          253..284
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          286..317
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          319..349
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          333..621
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          352..416
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          418..449
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(451,453,457..458,461..463,465..466,470,473,482,484,
                     486,490..491,494..496,498..499,503,506,515,517,519,
                     523..524,527..529,531..532,536,539)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          451..482
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          484..515
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          499..777
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          517..546
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          550..575
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          583..614
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          616..649
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          650..680
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          682..712
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(715,717,721..722,725..727,729..730,734,737,746,748,
                     750,754..755,758..760,762..763,767,770,779,781,783,
                     787..788,791..793,795..796,800,803)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          715..746
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          748..779
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          753..834
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          781..811
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          999..1101
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1324..1456
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1488..1571
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <3437..3713
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..4972
                     /gene="LOC144114148"
                     /coded_by="XM_077647698.1:881..15799"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 mgpgeptirq sreswigcli rllasqndqc dpsvylflrw ltllllravl sqmmppatdg
       61 nasflraara gnlekvldyl kgsidintsn anglnalhla akeghvnvvs ellkrganvn
      121 attkkgnsal hiaslagqee vvkllvqkqa nvnvqsqngf tplymaaqen hdgvvrflla
      181 nganqslate dgftplaval qqghdkvvav llendargkv rlpalhiask kddckaaall
      241 lhsehnpdvt sksgftplhi aahygnsnia sllldkgadv nfpakhqitp lhvaakwgks
      301 nmvklllekg akidastrdg ltplhcaars ghdqvveqll eknapitakt knglaplhma
      361 sqgdhvdsar illyhkapvd dvtvdyltal hvaahcghvg vakllldrra dpnaralngf
      421 tplhiackkn rikvvelllk hgasieatte sgltplhvas fmgcmniviy liqhganpdi
      481 ptvrgetplh laaranqtdi irillrngah vdakarelqt alhiasrlgn admvglllqh
      541 gaavdaptkd aytplhvaar egqdevaall ldhgaalaap tkkgftplhl aakygnlkva
      601 qlllqkdapv daqgkngvtp lhvaahydhv nvallllekg asphsaarng ytplhvaark
      661 dqmdiassll eygakpgaes ragftplhla aqeghadlaa llvehgadcd akakngltpm
      721 hlcaqedrve vatilakhga sldpttkagy tplhvachfg qtnmirfllr qganvnatts
      781 hgytplhqaa qqghtliinl llehraapna vtnqgqtala iaqrlgyisv vetlkvvtet
      841 ivttttttvt eekyrvvape tmhetfmsds edeaaednml gdqsfrylta demkslgdds
      901 lpidvtrder itesihitre pghpapltqe eerlsptqah tteaifvgny apdnvdlsrt
      961 pihagsllsw dgdscssayv lsptkrmwre ssklkwktfl vsflvdargg amrgcrhsgv
     1021 rviipprkaq mpmritcryl rkeklphppp llegeacasr ilevgpagak flgpvilevp
     1081 hfaslrgker eisilrsdng etwkehtlea seeavqevln esfegeelsa ledlqtnriv
     1141 rilttdfpqy favvsrtrqe vhavgpeggl lsstvvpqvq aifpegaltk kirvglqaqp
     1201 ipaelvtkll ghrvavspiv tveprrrkfh kpitltipvp qaatkgminq yagdaptlrl
     1261 lcsitggtnk aqwedvtgst pltfvndcvs ftttvsarfw lmdcrqvsea tkfatelyae
     1321 amhvpfmakf vvfakrqdpm eaqlrvfcmt ddkedktles qehftevaks rdvevlekks
     1381 qylefggnlv pvtksgeqlq lqfqafrenr lpfavrvkdp hqeplgrlaf mrqpraarat
     1441 eppqaplcnl nialpeyeas qnlselvtle kkygfveetg lakpelihra dlrlsdiare
     1501 lgsdwpalaa qldvpeqdva sirtecpsdl aqqallmlrl wmkraggrat gnnlekglrm
     1561 inredivnkc mfnvelvtdd vekavakvhl dqsgfdtfre elgtpkdtsl krdasldvsy
     1621 deqdlmkeae saeetssetg svhekhtgse akvdtsdqve epaveklela rreskrysqe
     1681 feqdeplpka mgfktkpsee tvvsstqvkg vhdngtvkht vttvtkttii qeveepagap
     1741 rpgesetwas dldtsqvsvs slpedertep tlsssattat adaealsgpl artqveekew
     1801 tetdpdsgev rhiiskttht tvtskkpseg aprgdttstt vshsskdghh gstpsapsec
     1861 deaesltffq kkeffeklse qsppketssa rlgstpsldt altrpavtlk prprseslts
     1921 qgeiieesiv faerlrlfqn gaqasplpsa sipqvdlvdp apkmeqklqg maeigdslqq
     1981 vpsavleglq steeksdtrl lvhtsdadke afdvrsvqva edfgdkqfsh mrvrmpapee
     2041 tvsktsicss dlpgdkpspl rdaykgslsa srqessqagk pplpsalggd sslclvheat
     2101 tdltlskagp pnqtnkditt fdrqpvreqs ptlrssqsqm lasglqmgiv agsgilqegi
     2161 kaspqsaile tfsssatemt glespeirgl pseevhagsa eeakgidagr igavlaaapv
     2221 ailpvrdiis gkqlemldia qvsdepnife gcvelpsqae sqtcpkekti mkteyadiqh
     2281 klssafgesl apvesksgvt qgvpkevlea ldrssqelia skldedserd tnilsatqgs
     2341 iphdvfpdhi estldnsaav pmrgaystps stavcltper leiqatreva eerqekpevl
     2401 valeayrkst lrrsgedsyd rnleefitvl eeyeplpagq lsdwkraspa fepsichnse
     2461 itsklppled kkeletvgsi ldpsenvsel lgnhaqsqml cedtglltde lgrtaghyvi
     2521 staesdrgec lptlseqapg kereeclnyd salgvrstsg lesslleesh eersldlars
     2581 edeqpslved smnvptessv idyqvvghsg vvkdaqtqpk dsdfdlheis rtgdktdlpl
     2641 scgddgkksa efsktvsgia admvikdtds ehlsqdmlhp tastvlcegr sdvpyssded
     2701 qhkelsryke velvipflrg tsssledeve dggsfssgdl qaekiaeflt tdpdtlnkvv
     2761 hfpqifqdyd tsahqepfvp tetemnvekh vgfvldemaa cstpcvplks drqteieeas
     2821 rivesvvhia sslveslhev nhetaikedi cdsalayaag splersevse tlsqagppst
     2881 iekdqrespf gvkemyqvlk esrgsedriq ysvtsfgdpl fadegdedqe rrrrgsptae
     2941 slddqaqiek cshevmmqll dgfsqsdvel aevrtedeap dieddvpspe agsheeksvp
     3001 ssqdnsgdlq pqlsteveig sqasgapapl seldyelcpd adqlrtsgde avatrvpple
     3061 perdeavaes shapsaaadp aasagrnagg rersplleas ppeetaycpe qdispaqeyp
     3121 eyltpdmqvr vgvspteaqp akekpvryse ssvesssevg spcppsqggt amafenvafa
     3181 gedvidatss pieessspag srspyevvrd ahaeeaaqta lveaekslrh avqeevrarp
     3241 qslvteesse qqehqiqpsi fneafdhspe esapekkqls egllgvgllt vsdttartpe
     3301 srrgdsdede eewadhvmsk irgrpipptp paspravqsa vevqgepwea pvveqervnv
     3361 esardmsaer dagvdvmgdr satqpqlqef rrtadgkrpq lehipseisd edlvereqsp
     3421 thitatcrgd rkdsssdrns spdsdgpkas gshrrsasgv gaialeavhs qdsggnekhs
     3481 hesrdkgaqe ddkiteepvd tmfvieeeap adsnrsesfc ddaderaart agglspsles
     3541 ppqkkdlddg sssgkpdaas pvvlrskskr tdkisdkrss rdldtghsgs etdhshyysf
     3601 eltsdsgktp gasrptssef dvnilsgqas seyetcatsq dgevsgsyat aaasshdtsf
     3661 atarsslsgs srssaysvds essghlgsme vseasetiva saheeldsdi esersksvlr
     3721 epydgeipes virggvehpf lpswqpalkd ssptlhgsag arpptaegvf spfqstvpse
     3781 pgrscvgwps sslstaesvr spskpevlng vsaplegqrr lrhnddfmas sheeeqlsvs
     3841 essatqseht wqtsvetmvd aveaqrfscd serhhrqstm qnsvslpfde avvaedshre
     3901 adddratppf qevngpveve yvpeyddlta rtlrpvrqqn apdgqteaqg dvalleasag
     3961 dastsreqhe eenstelree gfyaetfqgd lsseevesaa neallldksa csfyeychak
     4021 deyevcntrp avesaslkea ferpatpepv deiddqedeg nlaqgspnlh gkdyeafgha
     4081 saeesvpcsd kthiaqelse ndqykvfsds gnqsslhdet rsksvsssle ramtddvrly
     4141 tskcedyilq mhqtykygvg lpktsdggyl peegmdvenv kllesrlqeg nggqqdfpyd
     4201 lsyqqeyggd grvehgdlyt hqeveeegyd dersrtrgrr rlspisderg stpdydtlag
     4261 rktfgkgsek ddastsslme ferleaemas gtksssvgss dslsgrqhqg rsgagseqds
     4321 vsvnsltefe rlekeiveae gvdarlrgev aakldeideg hesqasdpgh etavydesga
     4381 tlllcasadv neepdgttdd aqlgdieaaq gqevawsrte rhflttrtes ghsvesvdrq
     4441 ssastatqfd adslrereid dsesnfgpdt pgwmqkecvd ldslhdslhd sehhdslqev
     4501 pskdkdslql repsadadsl qedhnelqds lyetrdidwd sfradetsgl ddtsrswhqf
     4561 psqirttels ptlsrpassp lvvpvvveri dtltwvdqdp akacvsdges lgsrqqehpr
     4621 ndalvsstds lepsssanth atyqcdtdsm msslnsaees tmvtstdtle hetlclasqg
     4681 kslslegglf sggdvteeqn trtpleggvs vddmeerfre cllrsaaspt neeqveerqt
     4741 mdekgnlivt rtvkrhvtse prlhtqtfmg pdaeqrsref vasfaameps aerdeyegfd
     4801 aagntvrvtq hvvvrptikt vtftgpdarl qmreymrsla ggqhssaent ptsdsgvgac
     4861 sslasppplm pdppaaapss acpglqrlsg gdqwmeaesa lddcsppvta trvittrttr
     4921 tmgpdgreql vtttteesgg eepearlrrs mqgvldsfma epptpphlde ee
//
LOCUS       XP_077503825            4971 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X33 [Amblyomma
            americanum].
ACCESSION   XP_077503825
VERSION     XP_077503825.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647699.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4971
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..4971
                     /product="uncharacterized protein LOC144114148 isoform
                     X33"
                     /calculated_mol_wt=539824
     Region          59..90
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          73..390
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(92,94,98..99,102..104,106..107,111,114,123,125,127,
                     131..132,135..137,139..140,144,147,156,158,160,164..165,
                     168..170,172..173,177,180)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          92..123
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          125..156
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          158..183
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          191..216
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          224..251
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(253,255,259..260,263..265,267..268,272,275,284,286,
                     288,292..293,296..298,300..301,305,308,317,319,321,
                     325..326,329..331,333..334,338,341)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          253..284
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          286..317
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          319..349
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          333..621
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          352..416
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          418..449
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(451,453,457..458,461..463,465..466,470,473,482,484,
                     486,490..491,494..496,498..499,503,506,515,517,519,
                     523..524,527..529,531..532,536,539)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          451..482
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          484..515
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          499..777
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          517..546
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          550..575
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          583..614
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          616..649
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          650..680
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          682..712
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(715,717,721..722,725..727,729..730,734,737,746,748,
                     750,754..755,758..760,762..763,767,770,779,781,783,
                     787..788,791..793,795..796,800,803)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          715..746
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          748..779
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          753..834
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          781..811
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          999..1101
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1324..1456
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1488..1571
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <3399..3712
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..4971
                     /gene="LOC144114148"
                     /coded_by="XM_077647699.1:880..15795"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 mgpgeptirq sreswigcli rllasqndqc dpsvylflrw ltllllravl sqmmppatdg
       61 nasflraara gnlekvldyl kgsidintsn anglnalhla akeghvnvvs ellkrganvn
      121 attkkgnsal hiaslagqee vvkllvqkqa nvnvqsqngf tplymaaqen hdgvvrflla
      181 nganqslate dgftplaval qqghdkvvav llendargkv rlpalhiask kddckaaall
      241 lhsehnpdvt sksgftplhi aahygnsnia sllldkgadv nfpakhqitp lhvaakwgks
      301 nmvklllekg akidastrdg ltplhcaars ghdqvveqll eknapitakt knglaplhma
      361 sqgdhvdsar illyhkapvd dvtvdyltal hvaahcghvg vakllldrra dpnaralngf
      421 tplhiackkn rikvvelllk hgasieatte sgltplhvas fmgcmniviy liqhganpdi
      481 ptvrgetplh laaranqtdi irillrngah vdakarelqt alhiasrlgn admvglllqh
      541 gaavdaptkd aytplhvaar egqdevaall ldhgaalaap tkkgftplhl aakygnlkva
      601 qlllqkdapv daqgkngvtp lhvaahydhv nvallllekg asphsaarng ytplhvaark
      661 dqmdiassll eygakpgaes ragftplhla aqeghadlaa llvehgadcd akakngltpm
      721 hlcaqedrve vatilakhga sldpttkagy tplhvachfg qtnmirfllr qganvnatts
      781 hgytplhqaa qqghtliinl llehraapna vtnqgqtala iaqrlgyisv vetlkvvtet
      841 ivttttttvt eekyrvvape tmhetfmsds edeaaednml gdqsfrylta demkslgdds
      901 lpidvtrder itesihitre pghpapltqe eerlsptqah tteaifvgny apdnvdlsrt
      961 pihagsllsw dgdscssayv lsptkrmwre ssklkwktfl vsflvdargg amrgcrhsgv
     1021 rviipprkaq mpmritcryl rkeklphppp llegeacasr ilevgpagak flgpvilevp
     1081 hfaslrgker eisilrsdng etwkehtlea seeavqevln esfegeelsa ledlqtnriv
     1141 rilttdfpqy favvsrtrqe vhavgpeggl lsstvvpqvq aifpegaltk kirvglqaqp
     1201 ipaelvtkll ghrvavspiv tveprrrkfh kpitltipvp qaatkgminq yagdaptlrl
     1261 lcsitggtnk aqwedvtgst pltfvndcvs ftttvsarfw lmdcrqvsea tkfatelyae
     1321 amhvpfmakf vvfakrqdpm eaqlrvfcmt ddkedktles qehftevaks rdvevlekks
     1381 qylefggnlv pvtksgeqlq lqfqafrenr lpfavrvkdp hqeplgrlaf mrqpraarat
     1441 eppqaplcnl nialpeyeas qnlselvtle kkygfveetg lakpelihra dlrlsdiare
     1501 lgsdwpalaa qldvpeqdva sirtecpsdl aqqallmlrl wmkraggrat gnnlekglrm
     1561 inredivnkc mfnvelvtdd vekavakvhl dqsgfdtfre elgtpkdtsl krdasldvsy
     1621 deqdlmkaes aeetssetgs vhekhtgsea kvdtsdqvee paveklelar reskrysqef
     1681 eqdeplpkam gfktkpseet vvsstqvkgv hdngtvkhtv ttvtkttiiq eveepagapr
     1741 pgesetwasd ldtsqvsvss lpedertept lsssattata daealsgpla rtqveekewt
     1801 etdpdsgevr hiisktthtt vtskkpsega prgdttsttv shsskdghhg stpsapsecd
     1861 eaesltffqk keffeklseq sppketssar lgstpsldta ltrpavtlkp rprsesltsq
     1921 geiieesivf aerlrlfqng aqasplpsas ipqvdlvdpa pkmeqklqgm aeigdslqqv
     1981 psavleglqs teeksdtrll vhtsdadkea fdvrsvqvae dfgdkqfshm rvrmpapeet
     2041 vsktsicssd lpgdkpsplr daykgslsas rqessqagkp plpsalggds slclvheatt
     2101 dltlskagpp nqtnkdittf drqpvreqsp tlrssqsqml asglqmgiva gsgilqegik
     2161 aspqsailet fsssatemtg lespeirglp seevhagsae eakgidagri gavlaaapva
     2221 ilpvrdiisg kqlemldiaq vsdepnifeg cvelpsqaes qtcpkektim kteyadiqhk
     2281 lssafgesla pvesksgvtq gvpkevleal drssqelias kldedserdt nilsatqgsi
     2341 phdvfpdhie stldnsaavp mrgaystpss tavcltperl eiqatrevae erqekpevlv
     2401 aleayrkstl rrsgedsydr nleefitvle eyeplpagql sdwkraspaf epsichnsei
     2461 tsklppledk keletvgsil dpsenvsell gnhaqsqmlc edtglltdel grtaghyvis
     2521 taesdrgecl ptlseqapgk ereeclnyds algvrstsgl esslleeshe ersldlarse
     2581 deqpslveds mnvptessvi dyqvvghsgv vkdaqtqpkd sdfdlheisr tgdktdlpls
     2641 cgddgkksae fsktvsgiaa dmvikdtdse hlsqdmlhpt astvlcegrs dvpyssdedq
     2701 hkelsrykev elvipflrgt sssledeved ggsfssgdlq aekiaefltt dpdtlnkvvh
     2761 fpqifqdydt sahqepfvpt etemnvekhv gfvldemaac stpcvplksd rqteieeasr
     2821 ivesvvhias slveslhevn hetaikedic dsalayaags plersevset lsqagppsti
     2881 ekdqrespfg vkemyqvlke srgsedriqy svtsfgdplf adegdedqer rrrgsptaes
     2941 lddqaqiekc shevmmqlld gfsqsdvela evrtedeapd ieddvpspea gsheeksvps
     3001 sqdnsgdlqp qlsteveigs qasgapapls eldyelcpda dqlrtsgdea vatrvpplep
     3061 erdeavaess hapsaaadpa asagrnaggr ersplleasp peetaycpeq dispaqeype
     3121 yltpdmqvrv gvspteaqpa kekpvryses svesssevgs pcppsqggta mafenvafag
     3181 edvidatssp ieessspags rspyevvrda haeeaaqtal veaekslrha vqeevrarpq
     3241 slvteesseq qehqiqpsif neafdhspee sapekkqlse gllgvglltv sdttartpes
     3301 rrgdsdedee ewadhvmski rgrpipptpp aspravqsav evqgepweap vveqervnve
     3361 sardmsaerd agvdvmgdrs atqpqlqefr rtadgkrpql ehipseisde dlvereqspt
     3421 hitatcrgdr kdsssdrnss pdsdgpkasg shrrsasgvg aialeavhsq dsggnekhsh
     3481 esrdkgaqed dkiteepvdt mfvieeeapa dsnrsesfcd daderaarta gglspslesp
     3541 pqkkdlddgs ssgkpdaasp vvlrskskrt dkisdkrssr dldtghsgse tdhshyysfe
     3601 ltsdsgktpg asrptssefd vnilsgqass eyetcatsqd gevsgsyata aasshdtsfa
     3661 tarsslsgss rssaysvdse ssghlgsmev seasetivas aheeldsdie sersksvlre
     3721 pydgeipesv irggvehpfl pswqpalkds sptlhgsaga rpptaegvfs pfqstvpsep
     3781 grscvgwpss slstaesvrs pskpevlngv saplegqrrl rhnddfmass heeeqlsvse
     3841 ssatqsehtw qtsvetmvda veaqrfscds erhhrqstmq nsvslpfdea vvaedshrea
     3901 dddratppfq evngpvevey vpeyddltar tlrpvrqqna pdgqteaqgd valleasagd
     3961 astsreqhee enstelreeg fyaetfqgdl sseevesaan eallldksac sfyeychakd
     4021 eyevcntrpa vesaslkeaf erpatpepvd eiddqedegn laqgspnlhg kdyeafghas
     4081 aeesvpcsdk thiaqelsen dqykvfsdsg nqsslhdetr sksvsssler amtddvrlyt
     4141 skcedyilqm hqtykygvgl pktsdggylp eegmdvenvk llesrlqegn ggqqdfpydl
     4201 syqqeyggdg rvehgdlyth qeveeegydd ersrtrgrrr lspisdergs tpdydtlagr
     4261 ktfgkgsekd dastsslmef erleaemasg tksssvgssd slsgrqhqgr sgagseqdsv
     4321 svnsltefer lekeiveaeg vdarlrgeva akldeidegh esqasdpghe tavydesgat
     4381 lllcasadvn eepdgttdda qlgdieaaqg qevawsrter hflttrtesg hsvesvdrqs
     4441 sastatqfda dslrereidd sesnfgpdtp gwmqkecvdl dslhdslhds ehhdslqevp
     4501 skdkdslqlr epsadadslq edhnelqdsl yetrdidwds fradetsgld dtsrswhqfp
     4561 sqirttelsp tlsrpasspl vvpvvverid tltwvdqdpa kacvsdgesl gsrqqehprn
     4621 dalvsstdsl epsssantha tyqcdtdsmm sslnsaeest mvtstdtleh etlclasqgk
     4681 slslegglfs ggdvteeqnt rtpleggvsv ddmeerfrec llrsaasptn eeqveerqtm
     4741 dekgnlivtr tvkrhvtsep rlhtqtfmgp daeqrsrefv asfaamepsa erdeyegfda
     4801 agntvrvtqh vvvrptiktv tftgpdarlq mreymrslag gqhssaentp tsdsgvgacs
     4861 slasppplmp dppaaapssa cpglqrlsgg dqwmeaesal ddcsppvtat rvittrttrt
     4921 mgpdgreqlv tttteesgge epearlrrsm qgvldsfmae pptpphldee e
//
LOCUS       XP_077503826            4941 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X34 [Amblyomma
            americanum].
ACCESSION   XP_077503826
VERSION     XP_077503826.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647700.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..4941
                     /product="uncharacterized protein LOC144114148 isoform
                     X34"
                     /calculated_mol_wt=536274
     Region          59..90
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          73..390
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(92,94,98..99,102..104,106..107,111,114,123,125,127,
                     131..132,135..137,139..140,144,147,156,158,160,164..165,
                     168..170,172..173,177,180)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          92..123
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          125..156
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          158..183
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          191..216
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          224..251
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(253,255,259..260,263..265,267..268,272,275,284,286,
                     288,292..293,296..298,300..301,305,308,317,319,321,
                     325..326,329..331,333..334,338,341)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          253..284
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          286..317
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          319..349
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          333..621
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          352..416
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          418..449
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(451,453,457..458,461..463,465..466,470,473,482,484,
                     486,490..491,494..496,498..499,503,506,515,517,519,
                     523..524,527..529,531..532,536,539)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          451..482
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          484..515
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          499..777
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          517..546
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          550..575
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          583..614
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          616..649
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          650..680
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          682..712
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(715,717,721..722,725..727,729..730,734,737,746,748,
                     750,754..755,758..760,762..763,767,770,779,781,783,
                     787..788,791..793,795..796,800,803)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          715..746
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          748..779
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          753..834
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          781..811
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          999..1101
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1324..1456
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1488..1571
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <3369..3682
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..4941
                     /gene="LOC144114148"
                     /coded_by="XM_077647700.1:883..15708"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 mgpgeptirq sreswigcli rllasqndqc dpsvylflrw ltllllravl sqmmppatdg
       61 nasflraara gnlekvldyl kgsidintsn anglnalhla akeghvnvvs ellkrganvn
      121 attkkgnsal hiaslagqee vvkllvqkqa nvnvqsqngf tplymaaqen hdgvvrflla
      181 nganqslate dgftplaval qqghdkvvav llendargkv rlpalhiask kddckaaall
      241 lhsehnpdvt sksgftplhi aahygnsnia sllldkgadv nfpakhqitp lhvaakwgks
      301 nmvklllekg akidastrdg ltplhcaars ghdqvveqll eknapitakt knglaplhma
      361 sqgdhvdsar illyhkapvd dvtvdyltal hvaahcghvg vakllldrra dpnaralngf
      421 tplhiackkn rikvvelllk hgasieatte sgltplhvas fmgcmniviy liqhganpdi
      481 ptvrgetplh laaranqtdi irillrngah vdakarelqt alhiasrlgn admvglllqh
      541 gaavdaptkd aytplhvaar egqdevaall ldhgaalaap tkkgftplhl aakygnlkva
      601 qlllqkdapv daqgkngvtp lhvaahydhv nvallllekg asphsaarng ytplhvaark
      661 dqmdiassll eygakpgaes ragftplhla aqeghadlaa llvehgadcd akakngltpm
      721 hlcaqedrve vatilakhga sldpttkagy tplhvachfg qtnmirfllr qganvnatts
      781 hgytplhqaa qqghtliinl llehraapna vtnqgqtala iaqrlgyisv vetlkvvtet
      841 ivttttttvt eekyrvvape tmhetfmsds edeaaednml gdqsfrylta demkslgdds
      901 lpidvtrder itesihitre pghpapltqe eerlsptqah tteaifvgny apdnvdlsrt
      961 pihagsllsw dgdscssayv lsptkrmwre ssklkwktfl vsflvdargg amrgcrhsgv
     1021 rviipprkaq mpmritcryl rkeklphppp llegeacasr ilevgpagak flgpvilevp
     1081 hfaslrgker eisilrsdng etwkehtlea seeavqevln esfegeelsa ledlqtnriv
     1141 rilttdfpqy favvsrtrqe vhavgpeggl lsstvvpqvq aifpegaltk kirvglqaqp
     1201 ipaelvtkll ghrvavspiv tveprrrkfh kpitltipvp qaatkgminq yagdaptlrl
     1261 lcsitggtnk aqwedvtgst pltfvndcvs ftttvsarfw lmdcrqvsea tkfatelyae
     1321 amhvpfmakf vvfakrqdpm eaqlrvfcmt ddkedktles qehftevaks rdvevlekks
     1381 qylefggnlv pvtksgeqlq lqfqafrenr lpfavrvkdp hqeplgrlaf mrqpraarat
     1441 eppqaplcnl nialpeyeas qnlselvtle kkygfveetg lakpelihra dlrlsdiare
     1501 lgsdwpalaa qldvpeqdva sirtecpsdl aqqallmlrl wmkraggrat gnnlekglrm
     1561 inredivnkc mfnvelvtdd vekavakvhl dqsgfdtfre elgtpkdtsl krdasldvsy
     1621 deqdlmkeae saeetssetg svhekhtgse akdeplpkam gfktkpseet vvsstqvkgv
     1681 hdngtvkhtv ttvtkttiiq eveepagapr pgesetwasd ldtsqvsvss lpedertept
     1741 lsssattata daealsgpla rtqveekewt etdpdsgevr hiisktthtt vtskkpsega
     1801 prgdttsttv shsskdghhg stpsapsecd eaesltffqk keffeklseq sppketssar
     1861 lgstpsldta ltrpavtlkp rprsesltsq geiieesivf aerlrlfqng aqasplpsas
     1921 ipqvdlvdpa pkmeqklqgm aeigdslqqv psavleglqs teeksdtrll vhtsdadkea
     1981 fdvrsvqvae dfgdkqfshm rvrmpapeet vsktsicssd lpgdkpsplr daykgslsas
     2041 rqessqagkp plpsalggds slclvheatt dltlskagpp nqtnkdittf drqpvreqsp
     2101 tlrssqsqml asglqmgiva gsgilqegik aspqsailet fsssatemtg lespeirglp
     2161 seevhagsae eakgidagri gavlaaapva ilpvrdiisg kqlemldiaq vsdepnifeg
     2221 cvelpsqaes qtcpkektim kteyadiqhk lssafgesla pvesksgvtq gvpkevleal
     2281 drssqelias kldedserdt nilsatqgsi phdvfpdhie stldnsaavp mrgaystpss
     2341 tavcltperl eiqatrevae erqekpevlv aleayrkstl rrsgedsydr nleefitvle
     2401 eyeplpagql sdwkraspaf epsichnsei tsklppledk keletvgsil dpsenvsell
     2461 gnhaqsqmlc edtglltdel grtaghyvis taesdrgecl ptlseqapgk ereeclnyds
     2521 algvrstsgl esslleeshe ersldlarse deqpslveds mnvptessvi dyqvvghsgv
     2581 vkdaqtqpkd sdfdlheisr tgdktdlpls cgddgkksae fsktvsgiaa dmvikdtdse
     2641 hlsqdmlhpt astvlcegrs dvpyssdedq hkelsrykev elvipflrgt sssledeved
     2701 ggsfssgdlq aekiaefltt dpdtlnkvvh fpqifqdydt sahqepfvpt etemnvekhv
     2761 gfvldemaac stpcvplksd rqteieeasr ivesvvhias slveslhevn hetaikedic
     2821 dsalayaags plersevset lsqagppsti ekdqrespfg vkemyqvlke srgsedriqy
     2881 svtsfgdplf adegdedqer rrrgsptaes lddqaqiekc shevmmqlld gfsqsdvela
     2941 evrtedeapd ieddvpspea gsheeksvps sqdnsgdlqp qlsteveigs qasgapapls
     3001 eldyelcpda dqlrtsgdea vatrvpplep erdeavaess hapsaaadpa asagrnaggr
     3061 ersplleasp peetaycpeq dispaqeype yltpdmqvrv gvspteaqpa kekpvryses
     3121 svesssevgs pcppsqggta mafenvafag edvidatssp ieessspags rspyevvrda
     3181 haeeaaqtal veaekslrha vqeevrarpq slvteesseq qehqiqpsif neafdhspee
     3241 sapekkqlse gllgvglltv sdttartpes rrgdsdedee ewadhvmski rgrpipptpp
     3301 aspravqsav evqgepweap vveqervnve sardmsaerd agvdvmgdrs atqpqlqefr
     3361 rtadgkrpql ehipseisde dlvereqspt hitatcrgdr kdsssdrnss pdsdgpkasg
     3421 shrrsasgvg aialeavhsq dsggnekhsh esrdkgaqed dkiteepvdt mfvieeeapa
     3481 dsnrsesfcd daderaarta gglspslesp pqkkdlddgs ssgkpdaasp vvlrskskrt
     3541 dkisdkrssr dldtghsgse tdhshyysfe ltsdsgktpg asrptssefd vnilsgqass
     3601 eyetcatsqd gevsgsyata aasshdtsfa tarsslsgss rssaysvdse ssghlgsmev
     3661 seasetivas aheeldsdie sersksvlre pydgeipesv irggvehpfl pswqpalkds
     3721 sptlhgsaga rpptaegvfs pfqstvpsep grscvgwpss slstaesvrs pskpevlngv
     3781 saplegqrrl rhnddfmass heeeqlsvse ssatqsehtw qtsvetmvda veaqrfscds
     3841 erhhrqstmq nsvslpfdea vvaedshrea dddratppfq evngpvevey vpeyddltar
     3901 tlrpvrqqna pdgqteaqgd valleasagd astsreqhee enstelreeg fyaetfqgdl
     3961 sseevesaan eallldksac sfyeychakd eyevcntrpa vesaslkeaf erpatpepvd
     4021 eiddqedegn laqgspnlhg kdyeafghas aeesvpcsdk thiaqelsen dqykvfsdsg
     4081 nqsslhdetr sksvsssler amtddvrlyt skcedyilqm hqtykygvgl pktsdggylp
     4141 eegmdvenvk llesrlqegn ggqqdfpydl syqqeyggdg rvehgdlyth qeveeegydd
     4201 ersrtrgrrr lspisdergs tpdydtlagr ktfgkgsekd dastsslmef erleaemasg
     4261 tksssvgssd slsgrqhqgr sgagseqdsv svnsltefer lekeiveaeg vdarlrgeva
     4321 akldeidegh esqasdpghe tavydesgat lllcasadvn eepdgttdda qlgdieaaqg
     4381 qevawsrter hflttrtesg hsvesvdrqs sastatqfda dslrereidd sesnfgpdtp
     4441 gwmqkecvdl dslhdslhds ehhdslqevp skdkdslqlr epsadadslq edhnelqdsl
     4501 yetrdidwds fradetsgld dtsrswhqfp sqirttelsp tlsrpasspl vvpvvverid
     4561 tltwvdqdpa kacvsdgesl gsrqqehprn dalvsstdsl epsssantha tyqcdtdsmm
     4621 sslnsaeest mvtstdtleh etlclasqgk slslegglfs ggdvteeqnt rtpleggvsv
     4681 ddmeerfrec llrsaasptn eeqveerqtm dekgnlivtr tvkrhvtsep rlhtqtfmgp
     4741 daeqrsrefv asfaamepsa erdeyegfda agntvrvtqh vvvrptiktv tftgpdarlq
     4801 mreymrslag gqhssaentp tsdsgvgacs slasppplmp dppaaapssa cpglqrlsgg
     4861 dqwmeaesal ddcsppvtat rvittrttrt mgpdgreqlv tttteesgge epearlrrsm
     4921 qgvldsfmae pptpphldee e
//
LOCUS       XP_077503828            4940 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114148 isoform X35 [Amblyomma
            americanum].
ACCESSION   XP_077503828
VERSION     XP_077503828.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647702.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..4940
                     /product="uncharacterized protein LOC144114148 isoform
                     X35"
                     /calculated_mol_wt=536145
     Region          59..90
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          73..390
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(92,94,98..99,102..104,106..107,111,114,123,125,127,
                     131..132,135..137,139..140,144,147,156,158,160,164..165,
                     168..170,172..173,177,180)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          92..123
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          125..156
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          158..183
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          191..216
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          224..251
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(253,255,259..260,263..265,267..268,272,275,284,286,
                     288,292..293,296..298,300..301,305,308,317,319,321,
                     325..326,329..331,333..334,338,341)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          253..284
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          286..317
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          319..349
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          333..621
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          352..416
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          418..449
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(451,453,457..458,461..463,465..466,470,473,482,484,
                     486,490..491,494..496,498..499,503,506,515,517,519,
                     523..524,527..529,531..532,536,539)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          451..482
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          484..515
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          499..777
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          517..546
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          550..575
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          583..614
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          616..649
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          650..680
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          682..712
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(715,717,721..722,725..727,729..730,734,737,746,748,
                     750,754..755,758..760,762..763,767,770,779,781,783,
                     787..788,791..793,795..796,800,803)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          715..746
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          748..779
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          753..834
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          781..811
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          999..1101
                     /region_name="ZU5"
                     /note="Domain present in ZO-1 and Unc5-like netrin
                     receptors; smart00218"
                     /db_xref="CDD:128514"
     Region          1324..1456
                     /region_name="UPA_2"
                     /note="UPA domain; pfam17809"
                     /db_xref="CDD:375346"
     Region          1488..1571
                     /region_name="Death_ank"
                     /note="Death domain associated with Ankyrins; cd08317"
                     /db_xref="CDD:260029"
     Region          <3368..3681
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     CDS             1..4940
                     /gene="LOC144114148"
                     /coded_by="XM_077647702.1:883..15705"
                     /db_xref="GeneID:144114148"
ORIGIN      
        1 mgpgeptirq sreswigcli rllasqndqc dpsvylflrw ltllllravl sqmmppatdg
       61 nasflraara gnlekvldyl kgsidintsn anglnalhla akeghvnvvs ellkrganvn
      121 attkkgnsal hiaslagqee vvkllvqkqa nvnvqsqngf tplymaaqen hdgvvrflla
      181 nganqslate dgftplaval qqghdkvvav llendargkv rlpalhiask kddckaaall
      241 lhsehnpdvt sksgftplhi aahygnsnia sllldkgadv nfpakhqitp lhvaakwgks
      301 nmvklllekg akidastrdg ltplhcaars ghdqvveqll eknapitakt knglaplhma
      361 sqgdhvdsar illyhkapvd dvtvdyltal hvaahcghvg vakllldrra dpnaralngf
      421 tplhiackkn rikvvelllk hgasieatte sgltplhvas fmgcmniviy liqhganpdi
      481 ptvrgetplh laaranqtdi irillrngah vdakarelqt alhiasrlgn admvglllqh
      541 gaavdaptkd aytplhvaar egqdevaall ldhgaalaap tkkgftplhl aakygnlkva
      601 qlllqkdapv daqgkngvtp lhvaahydhv nvallllekg asphsaarng ytplhvaark
      661 dqmdiassll eygakpgaes ragftplhla aqeghadlaa llvehgadcd akakngltpm
      721 hlcaqedrve vatilakhga sldpttkagy tplhvachfg qtnmirfllr qganvnatts
      781 hgytplhqaa qqghtliinl llehraapna vtnqgqtala iaqrlgyisv vetlkvvtet
      841 ivttttttvt eekyrvvape tmhetfmsds edeaaednml gdqsfrylta demkslgdds
      901 lpidvtrder itesihitre pghpapltqe eerlsptqah tteaifvgny apdnvdlsrt
      961 pihagsllsw dgdscssayv lsptkrmwre ssklkwktfl vsflvdargg amrgcrhsgv
     1021 rviipprkaq mpmritcryl rkeklphppp llegeacasr ilevgpagak flgpvilevp
     1081 hfaslrgker eisilrsdng etwkehtlea seeavqevln esfegeelsa ledlqtnriv
     1141 rilttdfpqy favvsrtrqe vhavgpeggl lsstvvpqvq aifpegaltk kirvglqaqp
     1201 ipaelvtkll ghrvavspiv tveprrrkfh kpitltipvp qaatkgminq yagdaptlrl
     1261 lcsitggtnk aqwedvtgst pltfvndcvs ftttvsarfw lmdcrqvsea tkfatelyae
     1321 amhvpfmakf vvfakrqdpm eaqlrvfcmt ddkedktles qehftevaks rdvevlekks
     1381 qylefggnlv pvtksgeqlq lqfqafrenr lpfavrvkdp hqeplgrlaf mrqpraarat
     1441 eppqaplcnl nialpeyeas qnlselvtle kkygfveetg lakpelihra dlrlsdiare
     1501 lgsdwpalaa qldvpeqdva sirtecpsdl aqqallmlrl wmkraggrat gnnlekglrm
     1561 inredivnkc mfnvelvtdd vekavakvhl dqsgfdtfre elgtpkdtsl krdasldvsy
     1621 deqdlmkaes aeetssetgs vhekhtgsea kdeplpkamg fktkpseetv vsstqvkgvh
     1681 dngtvkhtvt tvtkttiiqe veepagaprp gesetwasdl dtsqvsvssl pederteptl
     1741 sssattatad aealsgplar tqveekewte tdpdsgevrh iisktthttv tskkpsegap
     1801 rgdttsttvs hsskdghhgs tpsapsecde aesltffqkk effeklseqs ppketssarl
     1861 gstpsldtal trpavtlkpr prsesltsqg eiieesivfa erlrlfqnga qasplpsasi
     1921 pqvdlvdpap kmeqklqgma eigdslqqvp savleglqst eeksdtrllv htsdadkeaf
     1981 dvrsvqvaed fgdkqfshmr vrmpapeetv sktsicssdl pgdkpsplrd aykgslsasr
     2041 qessqagkpp lpsalggdss lclvheattd ltlskagppn qtnkdittfd rqpvreqspt
     2101 lrssqsqmla sglqmgivag sgilqegika spqsailetf sssatemtgl espeirglps
     2161 eevhagsaee akgidagrig avlaaapvai lpvrdiisgk qlemldiaqv sdepnifegc
     2221 velpsqaesq tcpkektimk teyadiqhkl ssafgeslap vesksgvtqg vpkevleald
     2281 rssqeliask ldedserdtn ilsatqgsip hdvfpdhies tldnsaavpm rgaystpsst
     2341 avcltperle iqatrevaee rqekpevlva leayrkstlr rsgedsydrn leefitvlee
     2401 yeplpagqls dwkraspafe psichnseit sklppledkk eletvgsild psenvsellg
     2461 nhaqsqmlce dtglltdelg rtaghyvist aesdrgeclp tlseqapgke reeclnydsa
     2521 lgvrstsgle sslleeshee rsldlarsed eqpslvedsm nvptessvid yqvvghsgvv
     2581 kdaqtqpkds dfdlheisrt gdktdlplsc gddgkksaef sktvsgiaad mvikdtdseh
     2641 lsqdmlhpta stvlcegrsd vpyssdedqh kelsrykeve lvipflrgts ssledevedg
     2701 gsfssgdlqa ekiaeflttd pdtlnkvvhf pqifqdydts ahqepfvpte temnvekhvg
     2761 fvldemaacs tpcvplksdr qteieeasri vesvvhiass lveslhevnh etaikedicd
     2821 salayaagsp lersevsetl sqagppstie kdqrespfgv kemyqvlkes rgsedriqys
     2881 vtsfgdplfa degdedqerr rrgsptaesl ddqaqiekcs hevmmqlldg fsqsdvelae
     2941 vrtedeapdi eddvpspeag sheeksvpss qdnsgdlqpq lsteveigsq asgapaplse
     3001 ldyelcpdad qlrtsgdeav atrvpplepe rdeavaessh apsaaadpaa sagrnaggre
     3061 rsplleaspp eetaycpeqd ispaqeypey ltpdmqvrvg vspteaqpak ekpvrysess
     3121 vesssevgsp cppsqggtam afenvafage dvidatsspi eessspagsr spyevvrdah
     3181 aeeaaqtalv eaekslrhav qeevrarpqs lvteesseqq ehqiqpsifn eafdhspees
     3241 apekkqlseg llgvglltvs dttartpesr rgdsdedeee wadhvmskir grpipptppa
     3301 spravqsave vqgepweapv veqervnves ardmsaerda gvdvmgdrsa tqpqlqefrr
     3361 tadgkrpqle hipseisded lvereqspth itatcrgdrk dsssdrnssp dsdgpkasgs
     3421 hrrsasgvga ialeavhsqd sggnekhshe srdkgaqedd kiteepvdtm fvieeeapad
     3481 snrsesfcdd aderaartag glspslespp qkkdlddgss sgkpdaaspv vlrskskrtd
     3541 kisdkrssrd ldtghsgset dhshyysfel tsdsgktpga srptssefdv nilsgqasse
     3601 yetcatsqdg evsgsyataa asshdtsfat arsslsgssr ssaysvdses sghlgsmevs
     3661 easetivasa heeldsdies ersksvlrep ydgeipesvi rggvehpflp swqpalkdss
     3721 ptlhgsagar pptaegvfsp fqstvpsepg rscvgwpsss lstaesvrsp skpevlngvs
     3781 aplegqrrlr hnddfmassh eeeqlsvses satqsehtwq tsvetmvdav eaqrfscdse
     3841 rhhrqstmqn svslpfdeav vaedshread ddratppfqe vngpveveyv peyddltart
     3901 lrpvrqqnap dgqteaqgdv alleasagda stsreqheee nstelreegf yaetfqgdls
     3961 seevesaane allldksacs fyeychakde yevcntrpav esaslkeafe rpatpepvde
     4021 iddqedegnl aqgspnlhgk dyeafghasa eesvpcsdkt hiaqelsend qykvfsdsgn
     4081 qsslhdetrs ksvssslera mtddvrlyts kcedyilqmh qtykygvglp ktsdggylpe
     4141 egmdvenvkl lesrlqegng gqqdfpydls yqqeyggdgr vehgdlythq eveeegydde
     4201 rsrtrgrrrl spisdergst pdydtlagrk tfgkgsekdd astsslmefe rleaemasgt
     4261 ksssvgssds lsgrqhqgrs gagseqdsvs vnslteferl ekeiveaegv darlrgevaa
     4321 kldeideghe sqasdpghet avydesgatl llcasadvne epdgttddaq lgdieaaqgq
     4381 evawsrterh flttrtesgh svesvdrqss astatqfdad slrereidds esnfgpdtpg
     4441 wmqkecvdld slhdslhdse hhdslqevps kdkdslqlre psadadslqe dhnelqdsly
     4501 etrdidwdsf radetsgldd tsrswhqfps qirttelspt lsrpassplv vpvvveridt
     4561 ltwvdqdpak acvsdgeslg srqqehprnd alvsstdsle psssanthat yqcdtdsmms
     4621 slnsaeestm vtstdtlehe tlclasqgks lslegglfsg gdvteeqntr tpleggvsvd
     4681 dmeerfrecl lrsaasptne eqveerqtmd ekgnlivtrt vkrhvtsepr lhtqtfmgpd
     4741 aeqrsrefva sfaamepsae rdeyegfdaa gntvrvtqhv vvrptiktvt ftgpdarlqm
     4801 reymrslagg qhssaentpt sdsgvgacss lasppplmpd ppaaapssac pglqrlsggd
     4861 qwmeaesald dcsppvtatr vittrttrtm gpdgreqlvt ttteesggee pearlrrsmq
     4921 gvldsfmaep ptpphldeee
//
LOCUS       XP_077503829             783 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114150 [Amblyomma americanum].
ACCESSION   XP_077503829
VERSION     XP_077503829.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647703.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..783
                     /product="uncharacterized protein LOC144114150"
                     /calculated_mol_wt=88888
     Region          312..406
                     /region_name="MULE"
                     /note="MULE transposase domain; pfam10551"
                     /db_xref="CDD:463145"
     CDS             1..783
                     /gene="LOC144114150"
                     /coded_by="XM_077647703.1:23..2374"
                     /db_xref="GeneID:144114150"
ORIGIN      
        1 masvfpnqse rqpehgdhdr rcgvcdkvft qrknmlqhmr mvhkletkga flhcdqcssl
       61 lstlenlvhh ynvahkfpae nlqlsfgndk efhdwkgqee asqvckfvis tgekrltngc
      121 trtyyacnrs gvarpgrgnk tramksqgsc rsgrtclsfl ivtrdegtvk vsyqrthygh
      181 dadvehlsmt dqerasiadd lskgvpmrti lkkiresvts kltplhltnr mdlhnikrqy
      241 nltvperchd ndavsvsiwv qsmrnkgqpv vrlykeqevv dptntfstkd falalmtepq
      301 eellkvlgtg tvcvdsthgt tgyqfelvtv lvldeygagl pmayficsti nektlsaffe
      361 slksstgals artfmtddas eyfkawstvm gppqhrllct whvdknwkrr isekikgkel
      421 avdvyhavrt llecpkkdev aellesflan kdprlqefiq yfrgnyahrl emwaysfrtg
      481 lgintnmyle rmhrtlkhsf legkknhrvd rlitalmdmt yeslmkrvqh ivkakrtskm
      541 srieknhrsa vaittpakrn edgtwtvqsq strgltyris kekgsaccpl kcsecmvcvh
      601 syscdceehr msltickhih cvviagapeg rdqkilkate avssnskase afhilesvqt
      661 aeastsmtks dlvlakmell keyvaeqeip qevhnkaskl ldpvlkiyeg tkkqemphts
      721 repankkieh qvrfhstkkq rlsssgtsfs kptelqkeni kkqilnsnae atviitspgh
      781 nyi
//
LOCUS       XP_077503830             113 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114153 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503830
VERSION     XP_077503830.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647704.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..113
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..113
                     /product="uncharacterized protein LOC144114153 isoform X1"
                     /calculated_mol_wt=12154
     CDS             1..113
                     /gene="LOC144114153"
                     /coded_by="XM_077647704.1:1091..1432"
                     /db_xref="GeneID:144114153"
ORIGIN      
        1 maalvnvasg svtapvaawh avvvacvrav rtanaskvaa scfwlpdtll lepnmlretp
       61 lsmnlvpvvs srslkkrnts sttawfssml aledptspqt mlllglwrwy hlc
//
LOCUS       XP_077503831              83 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114153 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503831
VERSION     XP_077503831.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647705.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..83
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..83
                     /product="uncharacterized protein LOC144114153 isoform X2"
                     /calculated_mol_wt=8180
     CDS             1..83
                     /gene="LOC144114153"
                     /coded_by="XM_077647705.1:272..523"
                     /db_xref="GeneID:144114153"
ORIGIN      
        1 mssqgalgtk fdeddllsss pahlsscqsf sgqrardkqp aeggqsatgt hqqqptappa
       61 sagssrpsia veevagrggg raa
//
LOCUS       XP_077503832              82 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114153 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503832
VERSION     XP_077503832.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647706.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..82
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..82
                     /product="uncharacterized protein LOC144114153 isoform X3"
                     /calculated_mol_wt=8093
     CDS             1..82
                     /gene="LOC144114153"
                     /coded_by="XM_077647706.1:298..546"
                     /db_xref="GeneID:144114153"
ORIGIN      
        1 mssqgalgtk fdeddllssp ahlsscqsfs gqrardkqpa eggqsatgth qqqptappas
       61 agssrpsiav eevagrgggr aa
//
LOCUS       XP_077503833             225 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114155 [Amblyomma americanum].
ACCESSION   XP_077503833
VERSION     XP_077503833.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647707.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..225
                     /product="uncharacterized protein LOC144114155"
                     /calculated_mol_wt=24781
     CDS             1..225
                     /gene="LOC144114155"
                     /coded_by="XM_077647707.1:581..1258"
                     /db_xref="GeneID:144114155"
ORIGIN      
        1 mfqrsaelll eppaalgdna gpchsrgvvl darahggtra rsasvllgal avcpspmkph
       61 hayslelqqp qwnhsfklqa wqpkserpdv ktnllailhr revtapdngr agqrgkrvgh
      121 spsrrlarrg rrlrprrphr qriegsrlvl laaghaaarv rrlvrdtave esgargrlqa
      181 seeaehlvdl rlvlfqagsl pswfssklgf vvprvlwyta gvfdg
//
LOCUS       XP_077503834             225 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114155 [Amblyomma americanum].
ACCESSION   XP_077503834
VERSION     XP_077503834.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647708.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..225
                     /product="uncharacterized protein LOC144114155"
                     /calculated_mol_wt=24781
     CDS             1..225
                     /gene="LOC144114155"
                     /coded_by="XM_077647708.1:702..1379"
                     /db_xref="GeneID:144114155"
ORIGIN      
        1 mfqrsaelll eppaalgdna gpchsrgvvl darahggtra rsasvllgal avcpspmkph
       61 hayslelqqp qwnhsfklqa wqpkserpdv ktnllailhr revtapdngr agqrgkrvgh
      121 spsrrlarrg rrlrprrphr qriegsrlvl laaghaaarv rrlvrdtave esgargrlqa
      181 seeaehlvdl rlvlfqagsl pswfssklgf vvprvlwyta gvfdg
//
LOCUS       XP_077503835             169 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114156 [Amblyomma americanum].
ACCESSION   XP_077503835
VERSION     XP_077503835.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647709.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..169
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..169
                     /product="uncharacterized protein LOC144114156"
                     /calculated_mol_wt=19099
     CDS             1..169
                     /gene="LOC144114156"
                     /coded_by="XM_077647709.1:789..1298"
                     /db_xref="GeneID:144114156"
ORIGIN      
        1 mkpphaysfe lqqpqwnhsf klqawqpkse rpdvktnlla ilhrrevtap dngragqrgk
       61 rvghspsrrl arrgrrlrpr rphrqriegs rlvllaagha aarvrrlard taveesgarg
      121 rlqasekaeh lvdlrlvlfq agslpswfss klgfvvprvl wytagvfdg
//
LOCUS       XP_077503836             169 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114156 [Amblyomma americanum].
ACCESSION   XP_077503836
VERSION     XP_077503836.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647710.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..169
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..169
                     /product="uncharacterized protein LOC144114156"
                     /calculated_mol_wt=19099
     CDS             1..169
                     /gene="LOC144114156"
                     /coded_by="XM_077647710.1:810..1319"
                     /db_xref="GeneID:144114156"
ORIGIN      
        1 mkpphaysfe lqqpqwnhsf klqawqpkse rpdvktnlla ilhrrevtap dngragqrgk
       61 rvghspsrrl arrgrrlrpr rphrqriegs rlvllaagha aarvrrlard taveesgarg
      121 rlqasekaeh lvdlrlvlfq agslpswfss klgfvvprvl wytagvfdg
//
LOCUS       XP_077503837             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114157 [Amblyomma americanum].
ACCESSION   XP_077503837
VERSION     XP_077503837.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647711.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144114157"
                     /calculated_mol_wt=14594
     CDS             1..141
                     /gene="LOC144114157"
                     /coded_by="XM_077647711.1:903..1328"
                     /db_xref="GeneID:144114157"
ORIGIN      
        1 maalvnvasg sviapaaash avvvacvrav rtanaskvaa scfwlpdtll lesavlretp
       61 lsknlvpvva srppkkrnts stsawfsskl glfqagslps wvsscrvscg irpaystasm
      121 asealsgwlq eaaqakeler h
//
LOCUS       XP_077503838             135 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114158 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503838
VERSION     XP_077503838.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647712.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..135
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..135
                     /product="uncharacterized protein LOC144114158 isoform X1"
                     /calculated_mol_wt=15468
     CDS             1..135
                     /gene="LOC144114158"
                     /coded_by="XM_077647712.1:404..811"
                     /db_xref="GeneID:144114158"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedrrr rhlnrrltat mkrlsgvhvl
       61 nhehrfldrg idqmskiiva alvkiygpgi asasrarpge vyvvhrcrrc gakghktdhc
      121 waycsprrha aagrg
//
LOCUS       XP_077503839             135 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114158 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503839
VERSION     XP_077503839.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647713.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..135
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..135
                     /product="uncharacterized protein LOC144114158 isoform X1"
                     /calculated_mol_wt=15468
     CDS             1..135
                     /gene="LOC144114158"
                     /coded_by="XM_077647713.1:340..747"
                     /db_xref="GeneID:144114158"
ORIGIN      
        1 mpcafqdlvf llqehvvlvf kilqlhyddp hkvrfedrrr rhlnrrltat mkrlsgvhvl
       61 nhehrfldrg idqmskiiva alvkiygpgi asasrarpge vyvvhrcrrc gakghktdhc
      121 waycsprrha aagrg
//
LOCUS       XP_077503840              85 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114158 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503840
VERSION     XP_077503840.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647714.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..85
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..85
                     /product="uncharacterized protein LOC144114158 isoform X2"
                     /calculated_mol_wt=9314
     CDS             1..85
                     /gene="LOC144114158"
                     /coded_by="XM_077647714.1:483..740"
                     /db_xref="GeneID:144114158"
ORIGIN      
        1 mkrlsgvhvl nhehrfldrg idqmskiiva alvkiygpgi asasrarpge vyvvhrcrrc
       61 gakghktdhc waycsprrha aagrg
//
LOCUS       XP_077503841              85 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114158 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503841
VERSION     XP_077503841.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647715.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..85
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..85
                     /product="uncharacterized protein LOC144114158 isoform X2"
                     /calculated_mol_wt=9314
     CDS             1..85
                     /gene="LOC144114158"
                     /coded_by="XM_077647715.1:419..676"
                     /db_xref="GeneID:144114158"
ORIGIN      
        1 mkrlsgvhvl nhehrfldrg idqmskiiva alvkiygpgi asasrarpge vyvvhrcrrc
       61 gakghktdhc waycsprrha aagrg
//
LOCUS       XP_077503842              85 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114158 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503842
VERSION     XP_077503842.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647716.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..85
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..85
                     /product="uncharacterized protein LOC144114158 isoform X2"
                     /calculated_mol_wt=9314
     CDS             1..85
                     /gene="LOC144114158"
                     /coded_by="XM_077647716.1:462..719"
                     /db_xref="GeneID:144114158"
ORIGIN      
        1 mkrlsgvhvl nhehrfldrg idqmskiiva alvkiygpgi asasrarpge vyvvhrcrrc
       61 gakghktdhc waycsprrha aagrg
//
LOCUS       XP_077503843              85 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114158 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503843
VERSION     XP_077503843.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647717.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..85
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..85
                     /product="uncharacterized protein LOC144114158 isoform X2"
                     /calculated_mol_wt=9314
     CDS             1..85
                     /gene="LOC144114158"
                     /coded_by="XM_077647717.1:398..655"
                     /db_xref="GeneID:144114158"
ORIGIN      
        1 mkrlsgvhvl nhehrfldrg idqmskiiva alvkiygpgi asasrarpge vyvvhrcrrc
       61 gakghktdhc waycsprrha aagrg
//
LOCUS       XP_077503844             124 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114160 [Amblyomma americanum].
ACCESSION   XP_077503844
VERSION     XP_077503844.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647718.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..124
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..124
                     /product="uncharacterized protein LOC144114160"
                     /calculated_mol_wt=13055
     CDS             1..124
                     /gene="LOC144114160"
                     /coded_by="XM_077647718.1:612..986"
                     /db_xref="GeneID:144114160"
ORIGIN      
        1 mdalvnvasg svtapvaawh avvvacvrav ltanaskvaa scfwlpdtll lestvlretp
       61 lsmnlvpvva srsakkrnts stsawfssml aledptspqt llllallrwy hlcsvsscrv
      121 sigk
//
LOCUS       XP_077503845            1639 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC144114167
            [Amblyomma americanum].
ACCESSION   XP_077503845
VERSION     XP_077503845.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647719.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 2 indels
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1639
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC144114167"
                     /calculated_mol_wt=181707
     Region          <901..>1058
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     CDS             1..1639
                     /gene="LOC144114167"
                     /coded_by="XM_077647719.1:164..5083"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 2 bases in 2 codons"
                     /db_xref="GeneID:144114167"
ORIGIN      
        1 mehkeasltr etkeekrrsl ptmadyghdi qdkpldmspf eegqmldcek dfmefllslp
       61 pepsttsksl ertrrhsksa kskpkvgldh ldnlcklmeq lgdlkeqnhk lqrrvqyled
      121 mkdlhdmqrl ldpdypyeer rashrtpspg ggsatraege dgvhrsrtqv alrskdcyrs
      181 rersksvgtq ddyadsrsik skrfpnwtrv kealglersa aecegaskga larvspsdgg
      241 sypdeclavp gvdgrratwe agspdlppqs cswseecfhg rmqdevlrqa stnyatlrpv
      301 epvrrksspt sptgirdgck ktafdtkcqc hldsdslypd vgdsygsrel ckkvpksawg
      361 rvknliqtgr etvkrqyppr aaksgssqtq frvearldsd ptscgapsdp edssasrgrd
      421 vrrrkvseqq gssarnsrts ddlqflpkeh skshtyiqna ayldaecegs rktrlrqqvs
      481 cpaedesskl tlaasersna pspqspqsqr ksrwnrvkkv ftgaaqekke eelpgasnps
      541 spvstavvfd lddlaaydge raapqrsedv sevlssnavp lchsatapvs slvqelqrnl
      601 sedfnqkile wermkaaksg rsprpvrkss aphrtvvaas kvardktkap rpekkkdlnw
      661 vnrelqkmer ekerlsreth kfqersvrlh rlrqallnap assnevlvrt sagefrfegi
      721 snaftkklye wetkkgvvpe fstialldas lqttcrngdk avpvrglsrs essvaepsnt
      781 rastsslpsm kpetllvpee ktevsrsran sepdlcssey kndnvasrpr tvsvgsaeag
      841 fahpevtwli dsgsgeiddq fetkpcqeag etkpsedsyy tlleenmlll eqlrakenvc
      901 arleanlerl derlelmasr hreeieryre slwnahrpag aqrgslaslk avadfryrle
      961 dlercnfrlq eerktlqeti rshsqeqaal iwdivdkvke lqamntdlys gdidgerrrt
     1021 slmrldadgl ariqqlseql lqsaqlleaa vadrssqvcq lrrellmcel asvgrgatks
     1081 arslrtlrsa sdgvlgavrs lhadegvsks glrisatspa vergpgpsvq lmesvhrlrs
     1141 ellrlfasdg nhpalgeart eaaapqgsst hqtlvsvpda adlsrrpngf renarhslas
     1201 ssespsrrsa pealasghdg sesssindse hvalglrlpr wtrdtlstss dsscleddsf
     1261 trmalgsgas rrlteshdwr rserdhelgr rvscygdyed dvfndpssts slevaytlaw
     1321 knrdppdlsa alrdrsnmtd vqtylnrdpa lvlqkarsds ptscnarnda peyfgrtgrh
     1381 sdsshrisfs lsdndhvkgd qetaagvasy nstcksktar rfskqrsatd pepdpsdlwi
     1441 seqscdpgak kgrrssrgts pskeslsrds yaesramsna lgqspcyipr tsltaaavrp
     1501 qsatgfvssk egaaafipas tsppspgqdl igvnedasie vnqtgeaglg eqttqhrkhr
     1561 qeppdksfls lrlrrpkkkd ysaigqlcrq tlpvsirdet espqealspt lprplsspvt
     1621 arsrsfsrsk wlplfatks
//
LOCUS       XP_077503846             303 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114168 [Amblyomma americanum].
ACCESSION   XP_077503846
VERSION     XP_077503846.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647720.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..303
                     /product="uncharacterized protein LOC144114168"
                     /calculated_mol_wt=33545
     Region          114..166
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          196..>303
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..303
                     /gene="LOC144114168"
                     /coded_by="XM_077647720.1:174..1085"
                     /db_xref="GeneID:144114168"
ORIGIN      
        1 mtgseepcgv aapvpdvpps phqaeevdga geetgvyvqt dmgmsdigal eqecirlndc
       61 vyttrsekvq lemteeslrs sepkvvfytg iasfttllai fkmlepfvsh svnnslakfq
      121 efvlflmkik lnlqnadlaf rfnvsestvs rifdkwlhva hcrlksqvmw persalqrtm
      181 pqafydsfgs dvaviidcfe ikierpssyl prsetwstyk gsntakylig iapqgivtfi
      241 segwggrvsd khitehcgll dnllpgdvvl adrgfnvads vgfyrarlhl paytkgkkql
      301 saa
//
LOCUS       XP_077503847            1261 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114169 [Amblyomma americanum].
ACCESSION   XP_077503847
VERSION     XP_077503847.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647721.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1261
                     /product="uncharacterized protein LOC144114169"
                     /calculated_mol_wt=135736
     Region          7..108
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          109..>190
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     CDS             1..1261
                     /gene="LOC144114169"
                     /coded_by="XM_077647721.1:275..4060"
                     /db_xref="GeneID:144114169"
ORIGIN      
        1 mcdprtvieg slkyrdgrkw ksrwcvvskl spvadcihvq lyrdskerck qgptkaslsl
       61 agclglesgf tldkeshtla licpdlvlal afdsrelliq wqvkiranva evqqflvqiw
      121 hapgraklas gparlhlqdh lfcltsglpp rllgswplke lrrfglvdgk fcfeggskcg
      181 kgeglhvlht nqaeelaeaf eaasrgklas rrklpcktsa metpsrmslq prsraqessq
      241 csssesrrpl lssgcsdvgs cdacfeivrf agdaptpddf frsrtfckvh yrwpscvsqg
      301 gaapsvsssd aesadtvslt isdiqepppg skeasdnqvs splpvsgsap avwtythcgk
      361 cgrhycarcs fpsqngrspd gsrwfppqwi gdlktnpqqa wttgaavhgr spsitetgsr
      421 ptsgvasqas sgrggkpsdr aslcsqssgt ssastsgast ssseynvprs fleslydqpr
      481 svihtdsdlf rnhpgtrpps gatpspvvds mlpavhgfga apcacsarva qtsaetplar
      541 sgssdsplvs pkrttpqprp actcnvrqqy qnydipraal anlylkeqsk naadfapdlq
      601 andpsldaam hydipkkyke haaqdspqga rtaglplacs cgnnlmpdsl smcqdfnacs
      661 ymraactcnr vtfwagnlvp cfqrsssedn qattiqcskg capsticrep lkrnrilgvm
      721 pdgkkleqcs dyvnchfies lplyqntcvl sslseecdse ngskgtlekk vlppaspsvn
      781 qkqfnspekt gplkktaqhn apstgtiges yevmsfrsss ehtsvvcdsn yiamrplepc
      841 kcdsdrgnss csvresadtk rtplplrpfm pyllpckeef pedvsseaes swknstkgsl
      901 aasrqrsaet pdkpsslada krkvltkkrs ssadgrqeae eisevdeavl mgrerrpafs
      961 klpfrarsqn lvdvtppssv pstpnnrsme nlyritfhrs adclklkgdf rfseedlsmd
     1021 agngdscdap sngtsikrss svpckpqqpd sisspsdsgv stslpesset vcelathech
     1081 mslhtslprs ldegttvshr rcdeskrfiq eafarcpqqa afglpgerya aglkgssess
     1141 ttssdsdyie tlslcssgsn gsgseylrce diaagkpaac pvscglkprs akeynavdrs
     1201 airhdmheeg lcgalhschr thhhapihlt lpkvvrsapp pspvqgngar ggatkpspaa
     1261 s
//
LOCUS       XP_077503848             466 aa            linear   INV 25-NOV-2025
DEFINITION  innexin unc-9-like [Amblyomma americanum].
ACCESSION   XP_077503848
VERSION     XP_077503848.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647722.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..466
                     /product="innexin unc-9-like"
                     /calculated_mol_wt=51862
     Region          20..403
                     /region_name="Innexin"
                     /note="pfam00876"
                     /db_xref="CDD:459975"
     CDS             1..466
                     /gene="LOC144114171"
                     /coded_by="XM_077647722.1:415..1815"
                     /db_xref="GeneID:144114171"
ORIGIN      
        1 msflfrfhdl iggihvpidd gvdklnrkyt lvvflflalp iftrqyigdp iecftptyft
       61 daqarfvnsy cwtastyylv sdaegdeaae appreppdpr vqpeeldyag fetppaggae
      121 rlrrvhvsyy qwaplillvq gccfhlpfvl wgacahsagv klrrllkras diaslppgcq
      181 qreallsefv dqfhtlvags agcctdpacg lplacrcvgg ptrylcllyl lvkslyvlnv
      241 glqfllltaf lgrgflrhgl elarrlaadg dwwnsprfpl qtlcqvraal qgglrtylcr
      301 cvlpinvfne kifsvvwfyl alllplnlas lllwlwrcfr cnrfafvrlc lwrtrmvslg
      361 elsrvsrkia vnylgwdgvf vlrliennhg samatvilgr lydfyanqmk aqddgsdvel
      421 gpmppsvaaq qsgamhpchg apchgggaap cahqfgarrp ggywgk
//
LOCUS       XP_077503849             466 aa            linear   INV 25-NOV-2025
DEFINITION  innexin unc-9-like [Amblyomma americanum].
ACCESSION   XP_077503849
VERSION     XP_077503849.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647723.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..466
                     /product="innexin unc-9-like"
                     /calculated_mol_wt=51862
     Region          20..403
                     /region_name="Innexin"
                     /note="pfam00876"
                     /db_xref="CDD:459975"
     CDS             1..466
                     /gene="LOC144114171"
                     /coded_by="XM_077647723.1:434..1834"
                     /db_xref="GeneID:144114171"
ORIGIN      
        1 msflfrfhdl iggihvpidd gvdklnrkyt lvvflflalp iftrqyigdp iecftptyft
       61 daqarfvnsy cwtastyylv sdaegdeaae appreppdpr vqpeeldyag fetppaggae
      121 rlrrvhvsyy qwaplillvq gccfhlpfvl wgacahsagv klrrllkras diaslppgcq
      181 qreallsefv dqfhtlvags agcctdpacg lplacrcvgg ptrylcllyl lvkslyvlnv
      241 glqfllltaf lgrgflrhgl elarrlaadg dwwnsprfpl qtlcqvraal qgglrtylcr
      301 cvlpinvfne kifsvvwfyl alllplnlas lllwlwrcfr cnrfafvrlc lwrtrmvslg
      361 elsrvsrkia vnylgwdgvf vlrliennhg samatvilgr lydfyanqmk aqddgsdvel
      421 gpmppsvaaq qsgamhpchg apchgggaap cahqfgarrp ggywgk
//
LOCUS       XP_077503850             466 aa            linear   INV 25-NOV-2025
DEFINITION  innexin unc-9-like [Amblyomma americanum].
ACCESSION   XP_077503850
VERSION     XP_077503850.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647724.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..466
                     /product="innexin unc-9-like"
                     /calculated_mol_wt=51862
     Region          20..403
                     /region_name="Innexin"
                     /note="pfam00876"
                     /db_xref="CDD:459975"
     CDS             1..466
                     /gene="LOC144114171"
                     /coded_by="XM_077647724.1:422..1822"
                     /db_xref="GeneID:144114171"
ORIGIN      
        1 msflfrfhdl iggihvpidd gvdklnrkyt lvvflflalp iftrqyigdp iecftptyft
       61 daqarfvnsy cwtastyylv sdaegdeaae appreppdpr vqpeeldyag fetppaggae
      121 rlrrvhvsyy qwaplillvq gccfhlpfvl wgacahsagv klrrllkras diaslppgcq
      181 qreallsefv dqfhtlvags agcctdpacg lplacrcvgg ptrylcllyl lvkslyvlnv
      241 glqfllltaf lgrgflrhgl elarrlaadg dwwnsprfpl qtlcqvraal qgglrtylcr
      301 cvlpinvfne kifsvvwfyl alllplnlas lllwlwrcfr cnrfafvrlc lwrtrmvslg
      361 elsrvsrkia vnylgwdgvf vlrliennhg samatvilgr lydfyanqmk aqddgsdvel
      421 gpmppsvaaq qsgamhpchg apchgggaap cahqfgarrp ggywgk
//
LOCUS       XP_077503851             466 aa            linear   INV 25-NOV-2025
DEFINITION  innexin unc-9-like [Amblyomma americanum].
ACCESSION   XP_077503851
VERSION     XP_077503851.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647725.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..466
                     /product="innexin unc-9-like"
                     /calculated_mol_wt=51862
     Region          20..403
                     /region_name="Innexin"
                     /note="pfam00876"
                     /db_xref="CDD:459975"
     CDS             1..466
                     /gene="LOC144114171"
                     /coded_by="XM_077647725.1:337..1737"
                     /db_xref="GeneID:144114171"
ORIGIN      
        1 msflfrfhdl iggihvpidd gvdklnrkyt lvvflflalp iftrqyigdp iecftptyft
       61 daqarfvnsy cwtastyylv sdaegdeaae appreppdpr vqpeeldyag fetppaggae
      121 rlrrvhvsyy qwaplillvq gccfhlpfvl wgacahsagv klrrllkras diaslppgcq
      181 qreallsefv dqfhtlvags agcctdpacg lplacrcvgg ptrylcllyl lvkslyvlnv
      241 glqfllltaf lgrgflrhgl elarrlaadg dwwnsprfpl qtlcqvraal qgglrtylcr
      301 cvlpinvfne kifsvvwfyl alllplnlas lllwlwrcfr cnrfafvrlc lwrtrmvslg
      361 elsrvsrkia vnylgwdgvf vlrliennhg samatvilgr lydfyanqmk aqddgsdvel
      421 gpmppsvaaq qsgamhpchg apchgggaap cahqfgarrp ggywgk
//
LOCUS       XP_077503853             154 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114172 [Amblyomma americanum].
ACCESSION   XP_077503853
VERSION     XP_077503853.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647727.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..154
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..154
                     /product="uncharacterized protein LOC144114172"
                     /calculated_mol_wt=16324
     CDS             1..154
                     /gene="LOC144114172"
                     /coded_by="XM_077647727.1:1..465"
                     /db_xref="GeneID:144114172"
ORIGIN      
        1 mqarrgglht asraapcisi apgpaaaagp ngsqtpwyrl gaplsrpprl varqgpppag
       61 pplsgqparr ppsspgddgr hqrevfrpeg slsgrgrvaw vpqdrreafl hgeappwrap
      121 vgtqrersas apavrtansr lrgerrshcq vfha
//
LOCUS       XP_077503854             579 aa            linear   INV 25-NOV-2025
DEFINITION  nucleostemin 4 [Amblyomma americanum].
ACCESSION   XP_077503854
VERSION     XP_077503854.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647728.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..579
                     /product="nucleostemin 4"
                     /calculated_mol_wt=65055
     Region          171..402
                     /region_name="HSR1_MMR1"
                     /note="A circularly permuted subfamily of the Ras GTPases;
                     cd01857"
                     /db_xref="CDD:206750"
     Region          211..490
                     /region_name="RbgA"
                     /note="Ribosome biogenesis GTPase RbgA [Translation,
                     ribosomal structure and biogenesis]; COG1161"
                     /db_xref="CDD:440775"
     Site            order(219..220,222..223,250,319..320,353..359,398)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206750"
     Site            219..222
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206750"
     Site            order(250,319..320)
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206750"
     Site            351..358
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206750"
     Site            376..383
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206750"
     Site            379
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206750"
     Site            395..398
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206750"
     Site            397..399
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206750"
     CDS             1..579
                     /gene="Ns4"
                     /coded_by="XM_077647728.1:233..1972"
                     /db_xref="GeneID:144114173"
ORIGIN      
        1 mpqgsrkvpf sakqkkiqlk qkrerkklgh egsgapkpqp aeqvvavqqe krslgdviri
       61 naegdvgqrs srvfryclqf rretpqeler rkkrarepyt pldesalevs peqfflpgsa
      121 ldmpkrppwn rsmskealea reaqyfreyl dkienafadk elsyfelnle twrqlwrvle
      181 mseivllvad irhpvvhfpp alyihvtkel gkrlvlvlnk vdlvpdtlta awikffhery
      241 pglvvlpfas yagmtakgkk gkrqgklrma seacrgllea cqemvgdqvd ltswrnkiee
      301 elveearddd dddeeehdal asaptevvap dtsfyeeerf kggrltlgfv gqpnvgkssl
      361 lnalvgrkvv svsrtpghtk hfqtiflten vrlcdcpglv fpslqpkalq vltgcfpiaq
      421 vrepysaiqf iaervdipsi lklkppegag dslkpwsafd lceawalerg yltakagrpd
      481 ayraansllr maldgrtlcl afrpqgysqc kgseadsall ahlarirgep pvksassdse
      541 qeesgdddse deededeeee eeedhfqsnk faalanaav
//
LOCUS       XP_077503855             579 aa            linear   INV 25-NOV-2025
DEFINITION  nucleostemin 4 [Amblyomma americanum].
ACCESSION   XP_077503855
VERSION     XP_077503855.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647729.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..579
                     /product="nucleostemin 4"
                     /calculated_mol_wt=65055
     Region          171..402
                     /region_name="HSR1_MMR1"
                     /note="A circularly permuted subfamily of the Ras GTPases;
                     cd01857"
                     /db_xref="CDD:206750"
     Region          211..490
                     /region_name="RbgA"
                     /note="Ribosome biogenesis GTPase RbgA [Translation,
                     ribosomal structure and biogenesis]; COG1161"
                     /db_xref="CDD:440775"
     Site            order(219..220,222..223,250,319..320,353..359,398)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206750"
     Site            219..222
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206750"
     Site            order(250,319..320)
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206750"
     Site            351..358
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206750"
     Site            376..383
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206750"
     Site            379
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206750"
     Site            395..398
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206750"
     Site            397..399
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206750"
     CDS             1..579
                     /gene="Ns4"
                     /coded_by="XM_077647729.1:165..1904"
                     /db_xref="GeneID:144114173"
ORIGIN      
        1 mpqgsrkvpf sakqkkiqlk qkrerkklgh egsgapkpqp aeqvvavqqe krslgdviri
       61 naegdvgqrs srvfryclqf rretpqeler rkkrarepyt pldesalevs peqfflpgsa
      121 ldmpkrppwn rsmskealea reaqyfreyl dkienafadk elsyfelnle twrqlwrvle
      181 mseivllvad irhpvvhfpp alyihvtkel gkrlvlvlnk vdlvpdtlta awikffhery
      241 pglvvlpfas yagmtakgkk gkrqgklrma seacrgllea cqemvgdqvd ltswrnkiee
      301 elveearddd dddeeehdal asaptevvap dtsfyeeerf kggrltlgfv gqpnvgkssl
      361 lnalvgrkvv svsrtpghtk hfqtiflten vrlcdcpglv fpslqpkalq vltgcfpiaq
      421 vrepysaiqf iaervdipsi lklkppegag dslkpwsafd lceawalerg yltakagrpd
      481 ayraansllr maldgrtlcl afrpqgysqc kgseadsall ahlarirgep pvksassdse
      541 qeesgdddse deededeeee eeedhfqsnk faalanaav
//
LOCUS       XP_077503856             579 aa            linear   INV 25-NOV-2025
DEFINITION  nucleostemin 4 [Amblyomma americanum].
ACCESSION   XP_077503856
VERSION     XP_077503856.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647730.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..579
                     /product="nucleostemin 4"
                     /calculated_mol_wt=65055
     Region          171..402
                     /region_name="HSR1_MMR1"
                     /note="A circularly permuted subfamily of the Ras GTPases;
                     cd01857"
                     /db_xref="CDD:206750"
     Region          211..490
                     /region_name="RbgA"
                     /note="Ribosome biogenesis GTPase RbgA [Translation,
                     ribosomal structure and biogenesis]; COG1161"
                     /db_xref="CDD:440775"
     Site            order(219..220,222..223,250,319..320,353..359,398)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206750"
     Site            219..222
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206750"
     Site            order(250,319..320)
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206750"
     Site            351..358
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206750"
     Site            376..383
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206750"
     Site            379
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206750"
     Site            395..398
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206750"
     Site            397..399
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206750"
     CDS             1..579
                     /gene="Ns4"
                     /coded_by="XM_077647730.1:146..1885"
                     /db_xref="GeneID:144114173"
ORIGIN      
        1 mpqgsrkvpf sakqkkiqlk qkrerkklgh egsgapkpqp aeqvvavqqe krslgdviri
       61 naegdvgqrs srvfryclqf rretpqeler rkkrarepyt pldesalevs peqfflpgsa
      121 ldmpkrppwn rsmskealea reaqyfreyl dkienafadk elsyfelnle twrqlwrvle
      181 mseivllvad irhpvvhfpp alyihvtkel gkrlvlvlnk vdlvpdtlta awikffhery
      241 pglvvlpfas yagmtakgkk gkrqgklrma seacrgllea cqemvgdqvd ltswrnkiee
      301 elveearddd dddeeehdal asaptevvap dtsfyeeerf kggrltlgfv gqpnvgkssl
      361 lnalvgrkvv svsrtpghtk hfqtiflten vrlcdcpglv fpslqpkalq vltgcfpiaq
      421 vrepysaiqf iaervdipsi lklkppegag dslkpwsafd lceawalerg yltakagrpd
      481 ayraansllr maldgrtlcl afrpqgysqc kgseadsall ahlarirgep pvksassdse
      541 qeesgdddse deededeeee eeedhfqsnk faalanaav
//
LOCUS       XP_077503857             306 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-tubulin N-acetyltransferase 1-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503857
VERSION     XP_077503857.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647731.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..306
                     /product="alpha-tubulin N-acetyltransferase 1-like isoform
                     X1"
                     /calculated_mol_wt=34343
     Region          44..198
                     /region_name="Acetyltransf_16"
                     /note="GNAT acetyltransferase, Mec-17; pfam05301"
                     /db_xref="CDD:461616"
     CDS             1..306
                     /gene="LOC144114174"
                     /coded_by="XM_077647731.1:131..1051"
                     /db_xref="GeneID:144114174"
ORIGIN      
        1 mefpftvdfl efefnviekg lkvqdaskvg plqdlpndeq hisvhsssrv qerlglvida
       61 lgeascaaqg lpapvttsdr lqnsghrlyv lvepgvrgai vlgllkvgfk qlflrdehsr
      121 lcqvrplcvl dfyvhhsrqr qgcgrrlfdr maqtekvtan qlaidqpsek mlafmerhyg
      181 ltkplpqsnh favfagffks sveqprppeq hitcekavrt rlrrphsyds slvgscldwp
      241 ccdgsqpvsr srqvyrgrqa qgraaesrgs wtptreplqp rcssrcsssw gifglpppdh
      301 craswh
//
LOCUS       XP_077503858             304 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-tubulin N-acetyltransferase 1-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503858
VERSION     XP_077503858.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647732.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..304
                     /product="alpha-tubulin N-acetyltransferase 1-like isoform
                     X2"
                     /calculated_mol_wt=34144
     Region          44..196
                     /region_name="Acetyltransf_16"
                     /note="GNAT acetyltransferase, Mec-17; pfam05301"
                     /db_xref="CDD:461616"
     CDS             1..304
                     /gene="LOC144114174"
                     /coded_by="XM_077647732.1:131..1045"
                     /db_xref="GeneID:144114174"
ORIGIN      
        1 mefpftvdfl efefnviekg lkvqdaskvg plqdlpndeq hisvhsssrv qerlglvida
       61 lgeascaglp apvttsdrlq nsghrlyvlv epgvrgaivl gllkvgfkql flrdehsrlc
      121 qvrplcvldf yvhhsrqrqg cgrrlfdrma qtekvtanql aidqpsekml afmerhyglt
      181 kplpqsnhfa vfagffkssv eqprppeqhi tcekavrtrl rrphsydssl vgscldwpcc
      241 dgsqpvsrsr qvyrgrqaqg raaesrgswt ptreplqprc ssrcssswgi fglpppdhcr
      301 aswh
//
LOCUS       XP_077503859             470 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114175 [Amblyomma americanum].
ACCESSION   XP_077503859
VERSION     XP_077503859.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647733.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..470
                     /product="uncharacterized protein LOC144114175"
                     /calculated_mol_wt=50590
     CDS             1..470
                     /gene="LOC144114175"
                     /coded_by="XM_077647733.1:300..1712"
                     /db_xref="GeneID:144114175"
ORIGIN      
        1 msggplllla rgrteaqsem ygrqeaampd wkrellrkkr tmqkafpswa alaagsggdr
       61 tvqkavhlgd aarvpaglvc pqsprgggpp smgedkgaqa elessedhew qygpgfvdrl
      121 rckflslslr epsglqqplr ayasvenlle pkssvrpasa pkpafqrars metlldplin
      181 edvviverss tttselprad ivrtckrife asvdnrrplr rrppvlrara plradkennq
      241 plarpraarp vanikplrkq deppanedep plktsltasr perrdaapgp lwpqlrqasp
      301 aatsvvfdfr gkdvkphvav trapcgspld sdeldssdep lpfpsgivfv genvvvggga
      361 llttrnkqlk iqfndsalvt ieyaaeechs prgnghdsea ptvngrncgg slgsytpsai
      421 ssgegfqlgl crpsrplpap eppvvpepee ppaapataws lsatssdllf
//
LOCUS       XP_077503860            1491 aa            linear   INV 25-NOV-2025
DEFINITION  CD109 antigen-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503860
VERSION     XP_077503860.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647734.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1491
                     /product="CD109 antigen-like isoform X1"
                     /calculated_mol_wt=165065
     Region          <132..1307
                     /region_name="YfaS"
                     /note="Uncharacterized conserved protein YfaS,
                     alpha-2-macroglobulin family [General function prediction
                     only]; COG2373"
                     /db_xref="CDD:441940"
     Region          938..1230
                     /region_name="A2M_2"
                     /note="Proteins similar to alpha2-macroglobulin (alpha
                     (2)-M). This group also contains the pregnancy zone
                     protein (PZP). Alpha(2)-M and PZP are broadly specific
                     proteinase inhibitors. Alpha (2)-M is a major carrier
                     protein in serum. The structural thioester...; cd02897"
                     /db_xref="CDD:239227"
     Site            order(952,954..955,958,998,1008,1011,1063,1065..1066,1216)
                     /site_type="other"
                     /note="surface patch"
                     /db_xref="CDD:239227"
     Site            952..955
                     /site_type="active"
                     /note="thioester region [active]"
                     /db_xref="CDD:239227"
     Site            order(1052,1056,1058,1066)
                     /site_type="other"
                     /note="specificity defining residues"
                     /db_xref="CDD:239227"
     Region          1337..1428
                     /region_name="A2M_recep"
                     /note="A-macroglobulin receptor binding domain; pfam07677"
                     /db_xref="CDD:462226"
     CDS             1..1491
                     /gene="LOC144114177"
                     /coded_by="XM_077647734.1:64..4539"
                     /db_xref="GeneID:144114177"
ORIGIN      
        1 mrvlcgacam grrvlcvpwl wlgrvrtdmg aavlfllacv plaasqsggv ytivapqklr
       61 pnlkyhvsas lsqsrspvdl qvtvsgrads gnfntitkna llhdrdtqvl sfeigdwgpg
      121 kykitaqgsg glnfrnetdl tyehksysvf tqtdkavykp gqkvlfrviv vdpyllptvt
      181 gamdvyiada kgnrihqwdr vftqrgiyss elqlsdqpvl gdwtinvdvl gqkfrksftv
      241 aeyvlptfev kvslpsyaty nksevvatvs aaytygkpvk gtvtltvtpr tryhqlnprp
      301 yeqyqtktti dgsvdipvai vrdlslktdf fsreieffal vteeltgrky nstsyitvhd
      361 keikvelvkt setfkpglky tcflkvayqd dspvndavnq lvlhygfnfn eelwqssqhw
      421 vpqngvirle fyppnenstv ilgfraefrg qtyyldgiyp akspsrsflq afmvteepkv
      481 gdliefevna tepldhlvye vvgrgdivfa qtlpvsgvrs yrfsfsatfr mapkarvlvy
      541 yvrkdgevva davnydvggi lrtpvhvqts iaetkpggqv nvlvstkpna yigvlgvdqs
      601 vlllkkgndl sqeqvieele sfdsgkkakl wppwyrrrrr slwwpgstta hdlfkdagma
      661 vltnglvyef ddatvyarkq virldtdvlt npvlppgglp eappqvpgri rlrqqypetw
      721 lwtnataspd graviastip dtitswvisa faldsltglg iapsqakvtv frpffvtasl
      781 pysvlrgesv aiqcvvfnyn kkpveaevtl ennrgefifs slsndvtgee nkerrtkkvm
      841 vpaqdgipvs flitplklgy idirvsatss lagdailkkl lvkpegskqy fnkailidrr
      901 npsapptian isipipknav pgserisisa igdllgpsvn nldqllvmph gcgeqnmldf
      961 vpnvvvldyl kranrlsppv qnkairnled gyqreltykr ddnsfsafgn tdksgstwlt
     1021 afvlksfaqa apytvidpkv lenatawlva rqqsdgsfne pgeiiykpmq sgagsgpalt
     1081 ayvliallen kakqthpdvi qaaeayllke lrttndpyvv slvtyalhls ghsaqdgafq
     1141 kllslatred dtiywkdpal avntsdkqsd yffkpsfkdv emtayalltl tergdignav
     1201 pvmrwlvskq nenggysstq dtvigiqala rvaasvvsqt iaieasvkyg dgrsrilkih
     1261 sgnalvlqrl elpsdlkyve ieatgfgvav lqvswsfnla vssespsffl nplldktste
     1321 nylqlsvcth yrgegdasnm avmevalpsg ylfdfdtlss ihrtkevrrv esqdadtnvv
     1381 iyfdrigrqe lcvtvpahre hkvanqkpvp vkvydyynla rsarmfysph katlcdiceg
     1441 vecgndcnav kqtksdatql eresepdgaa gihashlsva vlaitvalfw r
//
LOCUS       XP_077503861            1490 aa            linear   INV 25-NOV-2025
DEFINITION  CD109 antigen-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503861
VERSION     XP_077503861.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647735.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1490
                     /product="CD109 antigen-like isoform X2"
                     /calculated_mol_wt=164978
     Region          <132..1306
                     /region_name="YfaS"
                     /note="Uncharacterized conserved protein YfaS,
                     alpha-2-macroglobulin family [General function prediction
                     only]; COG2373"
                     /db_xref="CDD:441940"
     Region          937..1229
                     /region_name="A2M_2"
                     /note="Proteins similar to alpha2-macroglobulin (alpha
                     (2)-M). This group also contains the pregnancy zone
                     protein (PZP). Alpha(2)-M and PZP are broadly specific
                     proteinase inhibitors. Alpha (2)-M is a major carrier
                     protein in serum. The structural thioester...; cd02897"
                     /db_xref="CDD:239227"
     Site            order(951,953..954,957,997,1007,1010,1062,1064..1065,1215)
                     /site_type="other"
                     /note="surface patch"
                     /db_xref="CDD:239227"
     Site            951..954
                     /site_type="active"
                     /note="thioester region [active]"
                     /db_xref="CDD:239227"
     Site            order(1051,1055,1057,1065)
                     /site_type="other"
                     /note="specificity defining residues"
                     /db_xref="CDD:239227"
     Region          1336..1427
                     /region_name="A2M_recep"
                     /note="A-macroglobulin receptor binding domain; pfam07677"
                     /db_xref="CDD:462226"
     CDS             1..1490
                     /gene="LOC144114177"
                     /coded_by="XM_077647735.1:63..4535"
                     /db_xref="GeneID:144114177"
ORIGIN      
        1 mrvlcgacam grrvlcvpwl wlgrvrtdmg aavlfllacv plaasqsggv ytivapqklr
       61 pnlkyhvsas lsqsrspvdl qvtvsgrads gnfntitkna llhdrdtqvl sfeigdwgpg
      121 kykitaqgsg glnfrnetdl tyehksysvf tqtdkavykp gqkvlfrviv vdpyllptvt
      181 gamdvyiada kgnrihqwdr vftqrgiyss elqlsdqpvl gdwtinvdvl gqkfrksftv
      241 aeyvlptfev kvslpsyaty nksevvatvs aaytygkpvk gtvtltvtpr tryhqlnprp
      301 yeqyqtktti dgsvdipvai vrdlslktdf fsreieffal vteeltgrky nstsyitvhd
      361 keikvelvkt setfkpglky tcflkvayqd dspvndavnq lvlhygfnfn eelwqssqhw
      421 vpqngvirle fyppnenstv ilgfraefrg qtyyldgiyp akspsrsflq afmvteepkv
      481 gdliefevna tepldhlvye vvgrgdivfa qtlpvsgvrs yrfsfsatfr mapkarvlvy
      541 yvrkdgevva davnydvggi lrtpvhvqts iaetkpggqv nvlvstkpna yigvlgvdqs
      601 vlllkkgndl sqeqvieele sfdsgkkakl wppwyrrrrr slwwpgstta hdlfkdagma
      661 vltnglvyef ddaiyarkqv irldtdvltn pvlppgglpe appqvpgrir lrqqypetwl
      721 wtnataspdg raviastipd titswvisaf aldsltglgi apsqakvtvf rpffvtaslp
      781 ysvlrgesva iqcvvfnynk kpveaevtle nnrgefifss lsndvtgeen kerrtkkvmv
      841 paqdgipvsf litplklgyi dirvsatssl agdailkkll vkpegskqyf nkailidrrn
      901 psapptiani sipipknavp gserisisai gdllgpsvnn ldqllvmphg cgeqnmldfv
      961 pnvvvldylk ranrlsppvq nkairnledg yqreltykrd dnsfsafgnt dksgstwlta
     1021 fvlksfaqaa pytvidpkvl enatawlvar qqsdgsfnep geiiykpmqs gagsgpalta
     1081 yvliallenk akqthpdviq aaeayllkel rttndpyvvs lvtyalhlsg hsaqdgafqk
     1141 llslatredd tiywkdpala vntsdkqsdy ffkpsfkdve mtayalltlt ergdignavp
     1201 vmrwlvskqn enggysstqd tvigiqalar vaasvvsqti aieasvkygd grsrilkihs
     1261 gnalvlqrle lpsdlkyvei eatgfgvavl qvswsfnlav ssespsffln plldktsten
     1321 ylqlsvcthy rgegdasnma vmevalpsgy lfdfdtlssi hrtkevrrve sqdadtnvvi
     1381 yfdrigrqel cvtvpahreh kvanqkpvpv kvydyynlar sarmfysphk atlcdicegv
     1441 ecgndcnavk qtksdatqle resepdgaag ihashlsvav laitvalfwr
//
LOCUS       XP_077503862            1484 aa            linear   INV 25-NOV-2025
DEFINITION  CD109 antigen-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077503862
VERSION     XP_077503862.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647736.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1484
                     /product="CD109 antigen-like isoform X3"
                     /calculated_mol_wt=164356
     Region          <132..1300
                     /region_name="YfaS"
                     /note="Uncharacterized conserved protein YfaS,
                     alpha-2-macroglobulin family [General function prediction
                     only]; COG2373"
                     /db_xref="CDD:441940"
     Region          931..1223
                     /region_name="A2M_2"
                     /note="Proteins similar to alpha2-macroglobulin (alpha
                     (2)-M). This group also contains the pregnancy zone
                     protein (PZP). Alpha(2)-M and PZP are broadly specific
                     proteinase inhibitors. Alpha (2)-M is a major carrier
                     protein in serum. The structural thioester...; cd02897"
                     /db_xref="CDD:239227"
     Site            order(945,947..948,951,991,1001,1004,1056,1058..1059,1209)
                     /site_type="other"
                     /note="surface patch"
                     /db_xref="CDD:239227"
     Site            945..948
                     /site_type="active"
                     /note="thioester region [active]"
                     /db_xref="CDD:239227"
     Site            order(1045,1049,1051,1059)
                     /site_type="other"
                     /note="specificity defining residues"
                     /db_xref="CDD:239227"
     Region          1330..1421
                     /region_name="A2M_recep"
                     /note="A-macroglobulin receptor binding domain; pfam07677"
                     /db_xref="CDD:462226"
     CDS             1..1484
                     /gene="LOC144114177"
                     /coded_by="XM_077647736.1:64..4518"
                     /db_xref="GeneID:144114177"
ORIGIN      
        1 mrvlcgacam grrvlcvpwl wlgrvrtdmg aavlfllacv plaasqsggv ytivapqklr
       61 pnlkyhvsas lsqsrspvdl qvtvsgrads gnfntitkna llhdrdtqvl sfeigdwgpg
      121 kykitaqgsg glnfrnetdl tyehksysvf tqtdkavykp gqkvlfrviv vdpyllptvt
      181 gamdvyiada kgnrihqwdr vftqrgiyss elqlsdqpvl gdwtinvdvl gqkfrksftv
      241 aeyvlptfev kvslpsyaty nksevvatvs aaytygkpvk gtvtltvtpr tryhqlnprp
      301 yeqyqtktti dgsvdipvai vrdlslktdf fsreieffal vteeltgrky nstsyitvhd
      361 keikvelvkt setfkpglky tcflkvayqd dspvndavnq lvlhygfnfn eelwqssqhw
      421 vpqngvirle fyppnenstv ilgfraefrg qtyyldgiyp akspsrsflq afmvteepkv
      481 gdliefevna tepldhlvye vvgrgdivfa qtlpvsgvrs yrfsfsatfr mapkarvlvy
      541 yvrkdgevva davnydvggi lrtpvhvqts iaetkpggqv nvlvstkpna yigvlgvdqs
      601 vlllkkgndl sqeqvieele sfdsgkkakl wppwyrrrrr slwwpgstta hdlfkdagma
      661 vltnglvyef ddaiyyrsgf ggefelddsa ysggaagrne gkhrlrrffp etwlwnstlt
      721 gpdgravias tipdtitswv isafaldslt glgiapsqak vtvfrpffvt aslpysvlrg
      781 esvaiqcvvf nynkkpveae vtlennrgef ifsslsndvt geenkerrtk kvmvpaqdgi
      841 pvsflitplk lgyidirvsa tsslagdail kkllvkpegs kqyfnkaili drrnpsappt
      901 ianisipipk navpgseris isaigdllgp svnnldqllv mphgcgeqnm ldfvpnvvvl
      961 dylkranrls ppvqnkairn ledgyqrelt ykrddnsfsa fgntdksgst wltafvlksf
     1021 aqaapytvid pkvlenataw lvarqqsdgs fnepgeiiyk pmqsgagsgp altayvlial
     1081 lenkakqthp dviqaaeayl lkelrttndp yvvslvtyal hlsghsaqdg afqkllslat
     1141 reddtiywkd palavntsdk qsdyffkpsf kdvemtayal ltltergdig navpvmrwlv
     1201 skqnenggys stqdtvigiq alarvaasvv sqtiaieasv kygdgrsril kihsgnalvl
     1261 qrlelpsdlk yveieatgfg vavlqvswsf nlavssesps fflnplldkt stenylqlsv
     1321 cthyrgegda snmavmeval psgylfdfdt lssihrtkev rrvesqdadt nvviyfdrig
     1381 rqelcvtvpa hrehkvanqk pvpvkvydyy nlarsarmfy sphkatlcdi cegvecgndc
     1441 navkqtksda tqleresepd gaagihashl svavlaitva lfwr
//
LOCUS       XP_077503863            1463 aa            linear   INV 25-NOV-2025
DEFINITION  CD109 antigen-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077503863
VERSION     XP_077503863.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647737.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1463
                     /product="CD109 antigen-like isoform X4"
                     /calculated_mol_wt=161864
     Region          <104..1279
                     /region_name="YfaS"
                     /note="Uncharacterized conserved protein YfaS,
                     alpha-2-macroglobulin family [General function prediction
                     only]; COG2373"
                     /db_xref="CDD:441940"
     Region          910..1202
                     /region_name="A2M_2"
                     /note="Proteins similar to alpha2-macroglobulin (alpha
                     (2)-M). This group also contains the pregnancy zone
                     protein (PZP). Alpha(2)-M and PZP are broadly specific
                     proteinase inhibitors. Alpha (2)-M is a major carrier
                     protein in serum. The structural thioester...; cd02897"
                     /db_xref="CDD:239227"
     Site            order(924,926..927,930,970,980,983,1035,1037..1038,1188)
                     /site_type="other"
                     /note="surface patch"
                     /db_xref="CDD:239227"
     Site            924..927
                     /site_type="active"
                     /note="thioester region [active]"
                     /db_xref="CDD:239227"
     Site            order(1024,1028,1030,1038)
                     /site_type="other"
                     /note="specificity defining residues"
                     /db_xref="CDD:239227"
     Region          1309..1400
                     /region_name="A2M_recep"
                     /note="A-macroglobulin receptor binding domain; pfam07677"
                     /db_xref="CDD:462226"
     CDS             1..1463
                     /gene="LOC144114177"
                     /coded_by="XM_077647737.1:314..4705"
                     /db_xref="GeneID:144114177"
ORIGIN      
        1 mgaavlflla cvplaasqsg gvytivapqk lrpnlkyhvs aslsqsrspv dlqvtvsgra
       61 dsgnfntitk nallhdrdtq vlsfeigdwg pgkykitaqg sgglnfrnet dltyehksys
      121 vftqtdkavy kpgqkvlfrv ivvdpyllpt vtgamdvyia dakgnrihqw drvftqrgiy
      181 sselqlsdqp vlgdwtinvd vlgqkfrksf tvaeyvlptf evkvslpsya tynksevvat
      241 vsaaytygkp vkgtvtltvt prtryhqlnp rpyeqyqtkt tidgsvdipv aivrdlslkt
      301 dffsreieff alvteeltgr kynstsyitv hdkeikvelv ktsetfkpgl kytcflkvay
      361 qddspvndav nqlvlhygfn fneelwqssq hwvpqngvir lefyppnens tvilgfraef
      421 rgqtyyldgi ypakspsrsf lqafmvteep kvgdliefev natepldhlv yevvgrgdiv
      481 faqtlpvsgv rsyrfsfsat frmapkarvl vyyvrkdgev vadavnydvg gilrtpvhvq
      541 tsiaetkpgg qvnvlvstkp nayigvlgvd qsvlllkkgn dlsqeqviee lesfdsgkka
      601 klwppwyrrr rrslwwpgst tahdlfkdag mavltnglvy efddatvyar kqvirldtdv
      661 ltnpvlppgg lpeappqvpg rirlrqqype twlwtnatas pdgraviast ipdtitswvi
      721 safaldsltg lgiapsqakv tvfrpffvta slpysvlrge svaiqcvvfn ynkkpveaev
      781 tlennrgefi fsslsndvtg eenkerrtkk vmvpaqdgip vsflitplkl gyidirvsat
      841 sslagdailk kllvkpegsk qyfnkailid rrnpsappti anisipipkn avpgserisi
      901 saigdllgps vnnldqllvm phgcgeqnml dfvpnvvvld ylkranrlsp pvqnkairnl
      961 edgyqrelty krddnsfsaf gntdksgstw ltafvlksfa qaapytvidp kvlenatawl
     1021 varqqsdgsf nepgeiiykp mqsgagsgpa ltayvliall enkakqthpd viqaaeayll
     1081 kelrttndpy vvslvtyalh lsghsaqdga fqkllslatr eddtiywkdp alavntsdkq
     1141 sdyffkpsfk dvemtayall tltergdign avpvmrwlvs kqnenggyss tqdtvigiqa
     1201 larvaasvvs qtiaieasvk ygdgrsrilk ihsgnalvlq rlelpsdlky veieatgfgv
     1261 avlqvswsfn lavssespsf flnplldkts tenylqlsvc thyrgegdas nmavmevalp
     1321 sgylfdfdtl ssihrtkevr rvesqdadtn vviyfdrigr qelcvtvpah rehkvanqkp
     1381 vpvkvydyyn larsarmfys phkatlcdic egvecgndcn avkqtksdat qleresepdg
     1441 aagihashls vavlaitval fwr
//
LOCUS       XP_077503864            1463 aa            linear   INV 25-NOV-2025
DEFINITION  CD109 antigen-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077503864
VERSION     XP_077503864.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647738.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1463
                     /product="CD109 antigen-like isoform X4"
                     /calculated_mol_wt=161864
     Region          <104..1279
                     /region_name="YfaS"
                     /note="Uncharacterized conserved protein YfaS,
                     alpha-2-macroglobulin family [General function prediction
                     only]; COG2373"
                     /db_xref="CDD:441940"
     Region          910..1202
                     /region_name="A2M_2"
                     /note="Proteins similar to alpha2-macroglobulin (alpha
                     (2)-M). This group also contains the pregnancy zone
                     protein (PZP). Alpha(2)-M and PZP are broadly specific
                     proteinase inhibitors. Alpha (2)-M is a major carrier
                     protein in serum. The structural thioester...; cd02897"
                     /db_xref="CDD:239227"
     Site            order(924,926..927,930,970,980,983,1035,1037..1038,1188)
                     /site_type="other"
                     /note="surface patch"
                     /db_xref="CDD:239227"
     Site            924..927
                     /site_type="active"
                     /note="thioester region [active]"
                     /db_xref="CDD:239227"
     Site            order(1024,1028,1030,1038)
                     /site_type="other"
                     /note="specificity defining residues"
                     /db_xref="CDD:239227"
     Region          1309..1400
                     /region_name="A2M_recep"
                     /note="A-macroglobulin receptor binding domain; pfam07677"
                     /db_xref="CDD:462226"
     CDS             1..1463
                     /gene="LOC144114177"
                     /coded_by="XM_077647738.1:224..4615"
                     /db_xref="GeneID:144114177"
ORIGIN      
        1 mgaavlflla cvplaasqsg gvytivapqk lrpnlkyhvs aslsqsrspv dlqvtvsgra
       61 dsgnfntitk nallhdrdtq vlsfeigdwg pgkykitaqg sgglnfrnet dltyehksys
      121 vftqtdkavy kpgqkvlfrv ivvdpyllpt vtgamdvyia dakgnrihqw drvftqrgiy
      181 sselqlsdqp vlgdwtinvd vlgqkfrksf tvaeyvlptf evkvslpsya tynksevvat
      241 vsaaytygkp vkgtvtltvt prtryhqlnp rpyeqyqtkt tidgsvdipv aivrdlslkt
      301 dffsreieff alvteeltgr kynstsyitv hdkeikvelv ktsetfkpgl kytcflkvay
      361 qddspvndav nqlvlhygfn fneelwqssq hwvpqngvir lefyppnens tvilgfraef
      421 rgqtyyldgi ypakspsrsf lqafmvteep kvgdliefev natepldhlv yevvgrgdiv
      481 faqtlpvsgv rsyrfsfsat frmapkarvl vyyvrkdgev vadavnydvg gilrtpvhvq
      541 tsiaetkpgg qvnvlvstkp nayigvlgvd qsvlllkkgn dlsqeqviee lesfdsgkka
      601 klwppwyrrr rrslwwpgst tahdlfkdag mavltnglvy efddatvyar kqvirldtdv
      661 ltnpvlppgg lpeappqvpg rirlrqqype twlwtnatas pdgraviast ipdtitswvi
      721 safaldsltg lgiapsqakv tvfrpffvta slpysvlrge svaiqcvvfn ynkkpveaev
      781 tlennrgefi fsslsndvtg eenkerrtkk vmvpaqdgip vsflitplkl gyidirvsat
      841 sslagdailk kllvkpegsk qyfnkailid rrnpsappti anisipipkn avpgserisi
      901 saigdllgps vnnldqllvm phgcgeqnml dfvpnvvvld ylkranrlsp pvqnkairnl
      961 edgyqrelty krddnsfsaf gntdksgstw ltafvlksfa qaapytvidp kvlenatawl
     1021 varqqsdgsf nepgeiiykp mqsgagsgpa ltayvliall enkakqthpd viqaaeayll
     1081 kelrttndpy vvslvtyalh lsghsaqdga fqkllslatr eddtiywkdp alavntsdkq
     1141 sdyffkpsfk dvemtayall tltergdign avpvmrwlvs kqnenggyss tqdtvigiqa
     1201 larvaasvvs qtiaieasvk ygdgrsrilk ihsgnalvlq rlelpsdlky veieatgfgv
     1261 avlqvswsfn lavssespsf flnplldkts tenylqlsvc thyrgegdas nmavmevalp
     1321 sgylfdfdtl ssihrtkevr rvesqdadtn vviyfdrigr qelcvtvpah rehkvanqkp
     1381 vpvkvydyyn larsarmfys phkatlcdic egvecgndcn avkqtksdat qleresepdg
     1441 aagihashls vavlaitval fwr
//
LOCUS       XP_077503865            1463 aa            linear   INV 25-NOV-2025
DEFINITION  CD109 antigen-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077503865
VERSION     XP_077503865.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647739.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1463
                     /product="CD109 antigen-like isoform X4"
                     /calculated_mol_wt=161864
     Region          <104..1279
                     /region_name="YfaS"
                     /note="Uncharacterized conserved protein YfaS,
                     alpha-2-macroglobulin family [General function prediction
                     only]; COG2373"
                     /db_xref="CDD:441940"
     Region          910..1202
                     /region_name="A2M_2"
                     /note="Proteins similar to alpha2-macroglobulin (alpha
                     (2)-M). This group also contains the pregnancy zone
                     protein (PZP). Alpha(2)-M and PZP are broadly specific
                     proteinase inhibitors. Alpha (2)-M is a major carrier
                     protein in serum. The structural thioester...; cd02897"
                     /db_xref="CDD:239227"
     Site            order(924,926..927,930,970,980,983,1035,1037..1038,1188)
                     /site_type="other"
                     /note="surface patch"
                     /db_xref="CDD:239227"
     Site            924..927
                     /site_type="active"
                     /note="thioester region [active]"
                     /db_xref="CDD:239227"
     Site            order(1024,1028,1030,1038)
                     /site_type="other"
                     /note="specificity defining residues"
                     /db_xref="CDD:239227"
     Region          1309..1400
                     /region_name="A2M_recep"
                     /note="A-macroglobulin receptor binding domain; pfam07677"
                     /db_xref="CDD:462226"
     CDS             1..1463
                     /gene="LOC144114177"
                     /coded_by="XM_077647739.1:276..4667"
                     /db_xref="GeneID:144114177"
ORIGIN      
        1 mgaavlflla cvplaasqsg gvytivapqk lrpnlkyhvs aslsqsrspv dlqvtvsgra
       61 dsgnfntitk nallhdrdtq vlsfeigdwg pgkykitaqg sgglnfrnet dltyehksys
      121 vftqtdkavy kpgqkvlfrv ivvdpyllpt vtgamdvyia dakgnrihqw drvftqrgiy
      181 sselqlsdqp vlgdwtinvd vlgqkfrksf tvaeyvlptf evkvslpsya tynksevvat
      241 vsaaytygkp vkgtvtltvt prtryhqlnp rpyeqyqtkt tidgsvdipv aivrdlslkt
      301 dffsreieff alvteeltgr kynstsyitv hdkeikvelv ktsetfkpgl kytcflkvay
      361 qddspvndav nqlvlhygfn fneelwqssq hwvpqngvir lefyppnens tvilgfraef
      421 rgqtyyldgi ypakspsrsf lqafmvteep kvgdliefev natepldhlv yevvgrgdiv
      481 faqtlpvsgv rsyrfsfsat frmapkarvl vyyvrkdgev vadavnydvg gilrtpvhvq
      541 tsiaetkpgg qvnvlvstkp nayigvlgvd qsvlllkkgn dlsqeqviee lesfdsgkka
      601 klwppwyrrr rrslwwpgst tahdlfkdag mavltnglvy efddatvyar kqvirldtdv
      661 ltnpvlppgg lpeappqvpg rirlrqqype twlwtnatas pdgraviast ipdtitswvi
      721 safaldsltg lgiapsqakv tvfrpffvta slpysvlrge svaiqcvvfn ynkkpveaev
      781 tlennrgefi fsslsndvtg eenkerrtkk vmvpaqdgip vsflitplkl gyidirvsat
      841 sslagdailk kllvkpegsk qyfnkailid rrnpsappti anisipipkn avpgserisi
      901 saigdllgps vnnldqllvm phgcgeqnml dfvpnvvvld ylkranrlsp pvqnkairnl
      961 edgyqrelty krddnsfsaf gntdksgstw ltafvlksfa qaapytvidp kvlenatawl
     1021 varqqsdgsf nepgeiiykp mqsgagsgpa ltayvliall enkakqthpd viqaaeayll
     1081 kelrttndpy vvslvtyalh lsghsaqdga fqkllslatr eddtiywkdp alavntsdkq
     1141 sdyffkpsfk dvemtayall tltergdign avpvmrwlvs kqnenggyss tqdtvigiqa
     1201 larvaasvvs qtiaieasvk ygdgrsrilk ihsgnalvlq rlelpsdlky veieatgfgv
     1261 avlqvswsfn lavssespsf flnplldkts tenylqlsvc thyrgegdas nmavmevalp
     1321 sgylfdfdtl ssihrtkevr rvesqdadtn vviyfdrigr qelcvtvpah rehkvanqkp
     1381 vpvkvydyyn larsarmfys phkatlcdic egvecgndcn avkqtksdat qleresepdg
     1441 aagihashls vavlaitval fwr
//
LOCUS       XP_077503866            1463 aa            linear   INV 25-NOV-2025
DEFINITION  CD109 antigen-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077503866
VERSION     XP_077503866.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647740.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1463
                     /product="CD109 antigen-like isoform X4"
                     /calculated_mol_wt=161864
     Region          <104..1279
                     /region_name="YfaS"
                     /note="Uncharacterized conserved protein YfaS,
                     alpha-2-macroglobulin family [General function prediction
                     only]; COG2373"
                     /db_xref="CDD:441940"
     Region          910..1202
                     /region_name="A2M_2"
                     /note="Proteins similar to alpha2-macroglobulin (alpha
                     (2)-M). This group also contains the pregnancy zone
                     protein (PZP). Alpha(2)-M and PZP are broadly specific
                     proteinase inhibitors. Alpha (2)-M is a major carrier
                     protein in serum. The structural thioester...; cd02897"
                     /db_xref="CDD:239227"
     Site            order(924,926..927,930,970,980,983,1035,1037..1038,1188)
                     /site_type="other"
                     /note="surface patch"
                     /db_xref="CDD:239227"
     Site            924..927
                     /site_type="active"
                     /note="thioester region [active]"
                     /db_xref="CDD:239227"
     Site            order(1024,1028,1030,1038)
                     /site_type="other"
                     /note="specificity defining residues"
                     /db_xref="CDD:239227"
     Region          1309..1400
                     /region_name="A2M_recep"
                     /note="A-macroglobulin receptor binding domain; pfam07677"
                     /db_xref="CDD:462226"
     CDS             1..1463
                     /gene="LOC144114177"
                     /coded_by="XM_077647740.1:126..4517"
                     /db_xref="GeneID:144114177"
ORIGIN      
        1 mgaavlflla cvplaasqsg gvytivapqk lrpnlkyhvs aslsqsrspv dlqvtvsgra
       61 dsgnfntitk nallhdrdtq vlsfeigdwg pgkykitaqg sgglnfrnet dltyehksys
      121 vftqtdkavy kpgqkvlfrv ivvdpyllpt vtgamdvyia dakgnrihqw drvftqrgiy
      181 sselqlsdqp vlgdwtinvd vlgqkfrksf tvaeyvlptf evkvslpsya tynksevvat
      241 vsaaytygkp vkgtvtltvt prtryhqlnp rpyeqyqtkt tidgsvdipv aivrdlslkt
      301 dffsreieff alvteeltgr kynstsyitv hdkeikvelv ktsetfkpgl kytcflkvay
      361 qddspvndav nqlvlhygfn fneelwqssq hwvpqngvir lefyppnens tvilgfraef
      421 rgqtyyldgi ypakspsrsf lqafmvteep kvgdliefev natepldhlv yevvgrgdiv
      481 faqtlpvsgv rsyrfsfsat frmapkarvl vyyvrkdgev vadavnydvg gilrtpvhvq
      541 tsiaetkpgg qvnvlvstkp nayigvlgvd qsvlllkkgn dlsqeqviee lesfdsgkka
      601 klwppwyrrr rrslwwpgst tahdlfkdag mavltnglvy efddatvyar kqvirldtdv
      661 ltnpvlppgg lpeappqvpg rirlrqqype twlwtnatas pdgraviast ipdtitswvi
      721 safaldsltg lgiapsqakv tvfrpffvta slpysvlrge svaiqcvvfn ynkkpveaev
      781 tlennrgefi fsslsndvtg eenkerrtkk vmvpaqdgip vsflitplkl gyidirvsat
      841 sslagdailk kllvkpegsk qyfnkailid rrnpsappti anisipipkn avpgserisi
      901 saigdllgps vnnldqllvm phgcgeqnml dfvpnvvvld ylkranrlsp pvqnkairnl
      961 edgyqrelty krddnsfsaf gntdksgstw ltafvlksfa qaapytvidp kvlenatawl
     1021 varqqsdgsf nepgeiiykp mqsgagsgpa ltayvliall enkakqthpd viqaaeayll
     1081 kelrttndpy vvslvtyalh lsghsaqdga fqkllslatr eddtiywkdp alavntsdkq
     1141 sdyffkpsfk dvemtayall tltergdign avpvmrwlvs kqnenggyss tqdtvigiqa
     1201 larvaasvvs qtiaieasvk ygdgrsrilk ihsgnalvlq rlelpsdlky veieatgfgv
     1261 avlqvswsfn lavssespsf flnplldkts tenylqlsvc thyrgegdas nmavmevalp
     1321 sgylfdfdtl ssihrtkevr rvesqdadtn vviyfdrigr qelcvtvpah rehkvanqkp
     1381 vpvkvydyyn larsarmfys phkatlcdic egvecgndcn avkqtksdat qleresepdg
     1441 aagihashls vavlaitval fwr
//
LOCUS       XP_077503867            1463 aa            linear   INV 25-NOV-2025
DEFINITION  CD109 antigen-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077503867
VERSION     XP_077503867.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647741.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1463
                     /product="CD109 antigen-like isoform X4"
                     /calculated_mol_wt=161864
     Region          <104..1279
                     /region_name="YfaS"
                     /note="Uncharacterized conserved protein YfaS,
                     alpha-2-macroglobulin family [General function prediction
                     only]; COG2373"
                     /db_xref="CDD:441940"
     Region          910..1202
                     /region_name="A2M_2"
                     /note="Proteins similar to alpha2-macroglobulin (alpha
                     (2)-M). This group also contains the pregnancy zone
                     protein (PZP). Alpha(2)-M and PZP are broadly specific
                     proteinase inhibitors. Alpha (2)-M is a major carrier
                     protein in serum. The structural thioester...; cd02897"
                     /db_xref="CDD:239227"
     Site            order(924,926..927,930,970,980,983,1035,1037..1038,1188)
                     /site_type="other"
                     /note="surface patch"
                     /db_xref="CDD:239227"
     Site            924..927
                     /site_type="active"
                     /note="thioester region [active]"
                     /db_xref="CDD:239227"
     Site            order(1024,1028,1030,1038)
                     /site_type="other"
                     /note="specificity defining residues"
                     /db_xref="CDD:239227"
     Region          1309..1400
                     /region_name="A2M_recep"
                     /note="A-macroglobulin receptor binding domain; pfam07677"
                     /db_xref="CDD:462226"
     CDS             1..1463
                     /gene="LOC144114177"
                     /coded_by="XM_077647741.1:146..4537"
                     /db_xref="GeneID:144114177"
ORIGIN      
        1 mgaavlflla cvplaasqsg gvytivapqk lrpnlkyhvs aslsqsrspv dlqvtvsgra
       61 dsgnfntitk nallhdrdtq vlsfeigdwg pgkykitaqg sgglnfrnet dltyehksys
      121 vftqtdkavy kpgqkvlfrv ivvdpyllpt vtgamdvyia dakgnrihqw drvftqrgiy
      181 sselqlsdqp vlgdwtinvd vlgqkfrksf tvaeyvlptf evkvslpsya tynksevvat
      241 vsaaytygkp vkgtvtltvt prtryhqlnp rpyeqyqtkt tidgsvdipv aivrdlslkt
      301 dffsreieff alvteeltgr kynstsyitv hdkeikvelv ktsetfkpgl kytcflkvay
      361 qddspvndav nqlvlhygfn fneelwqssq hwvpqngvir lefyppnens tvilgfraef
      421 rgqtyyldgi ypakspsrsf lqafmvteep kvgdliefev natepldhlv yevvgrgdiv
      481 faqtlpvsgv rsyrfsfsat frmapkarvl vyyvrkdgev vadavnydvg gilrtpvhvq
      541 tsiaetkpgg qvnvlvstkp nayigvlgvd qsvlllkkgn dlsqeqviee lesfdsgkka
      601 klwppwyrrr rrslwwpgst tahdlfkdag mavltnglvy efddatvyar kqvirldtdv
      661 ltnpvlppgg lpeappqvpg rirlrqqype twlwtnatas pdgraviast ipdtitswvi
      721 safaldsltg lgiapsqakv tvfrpffvta slpysvlrge svaiqcvvfn ynkkpveaev
      781 tlennrgefi fsslsndvtg eenkerrtkk vmvpaqdgip vsflitplkl gyidirvsat
      841 sslagdailk kllvkpegsk qyfnkailid rrnpsappti anisipipkn avpgserisi
      901 saigdllgps vnnldqllvm phgcgeqnml dfvpnvvvld ylkranrlsp pvqnkairnl
      961 edgyqrelty krddnsfsaf gntdksgstw ltafvlksfa qaapytvidp kvlenatawl
     1021 varqqsdgsf nepgeiiykp mqsgagsgpa ltayvliall enkakqthpd viqaaeayll
     1081 kelrttndpy vvslvtyalh lsghsaqdga fqkllslatr eddtiywkdp alavntsdkq
     1141 sdyffkpsfk dvemtayall tltergdign avpvmrwlvs kqnenggyss tqdtvigiqa
     1201 larvaasvvs qtiaieasvk ygdgrsrilk ihsgnalvlq rlelpsdlky veieatgfgv
     1261 avlqvswsfn lavssespsf flnplldkts tenylqlsvc thyrgegdas nmavmevalp
     1321 sgylfdfdtl ssihrtkevr rvesqdadtn vviyfdrigr qelcvtvpah rehkvanqkp
     1381 vpvkvydyyn larsarmfys phkatlcdic egvecgndcn avkqtksdat qleresepdg
     1441 aagihashls vavlaitval fwr
//
LOCUS       XP_077503868            1462 aa            linear   INV 25-NOV-2025
DEFINITION  CD109 antigen-like isoform X5 [Amblyomma americanum].
ACCESSION   XP_077503868
VERSION     XP_077503868.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647742.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1462
                     /product="CD109 antigen-like isoform X5"
                     /calculated_mol_wt=161777
     Region          <104..1278
                     /region_name="YfaS"
                     /note="Uncharacterized conserved protein YfaS,
                     alpha-2-macroglobulin family [General function prediction
                     only]; COG2373"
                     /db_xref="CDD:441940"
     Region          909..1201
                     /region_name="A2M_2"
                     /note="Proteins similar to alpha2-macroglobulin (alpha
                     (2)-M). This group also contains the pregnancy zone
                     protein (PZP). Alpha(2)-M and PZP are broadly specific
                     proteinase inhibitors. Alpha (2)-M is a major carrier
                     protein in serum. The structural thioester...; cd02897"
                     /db_xref="CDD:239227"
     Site            order(923,925..926,929,969,979,982,1034,1036..1037,1187)
                     /site_type="other"
                     /note="surface patch"
                     /db_xref="CDD:239227"
     Site            923..926
                     /site_type="active"
                     /note="thioester region [active]"
                     /db_xref="CDD:239227"
     Site            order(1023,1027,1029,1037)
                     /site_type="other"
                     /note="specificity defining residues"
                     /db_xref="CDD:239227"
     Region          1308..1399
                     /region_name="A2M_recep"
                     /note="A-macroglobulin receptor binding domain; pfam07677"
                     /db_xref="CDD:462226"
     CDS             1..1462
                     /gene="LOC144114177"
                     /coded_by="XM_077647742.1:251..4639"
                     /db_xref="GeneID:144114177"
ORIGIN      
        1 mgaavlflla cvplaasqsg gvytivapqk lrpnlkyhvs aslsqsrspv dlqvtvsgra
       61 dsgnfntitk nallhdrdtq vlsfeigdwg pgkykitaqg sgglnfrnet dltyehksys
      121 vftqtdkavy kpgqkvlfrv ivvdpyllpt vtgamdvyia dakgnrihqw drvftqrgiy
      181 sselqlsdqp vlgdwtinvd vlgqkfrksf tvaeyvlptf evkvslpsya tynksevvat
      241 vsaaytygkp vkgtvtltvt prtryhqlnp rpyeqyqtkt tidgsvdipv aivrdlslkt
      301 dffsreieff alvteeltgr kynstsyitv hdkeikvelv ktsetfkpgl kytcflkvay
      361 qddspvndav nqlvlhygfn fneelwqssq hwvpqngvir lefyppnens tvilgfraef
      421 rgqtyyldgi ypakspsrsf lqafmvteep kvgdliefev natepldhlv yevvgrgdiv
      481 faqtlpvsgv rsyrfsfsat frmapkarvl vyyvrkdgev vadavnydvg gilrtpvhvq
      541 tsiaetkpgg qvnvlvstkp nayigvlgvd qsvlllkkgn dlsqeqviee lesfdsgkka
      601 klwppwyrrr rrslwwpgst tahdlfkdag mavltnglvy efddaiyark qvirldtdvl
      661 tnpvlppggl peappqvpgr irlrqqypet wlwtnatasp dgraviasti pdtitswvis
      721 afaldsltgl giapsqakvt vfrpffvtas lpysvlrges vaiqcvvfny nkkpveaevt
      781 lennrgefif sslsndvtge enkerrtkkv mvpaqdgipv sflitplklg yidirvsats
      841 slagdailkk llvkpegskq yfnkailidr rnpsapptia nisipipkna vpgserisis
      901 aigdllgpsv nnldqllvmp hgcgeqnmld fvpnvvvldy lkranrlspp vqnkairnle
      961 dgyqreltyk rddnsfsafg ntdksgstwl tafvlksfaq aapytvidpk vlenatawlv
     1021 arqqsdgsfn epgeiiykpm qsgagsgpal tayvlialle nkakqthpdv iqaaeayllk
     1081 elrttndpyv vslvtyalhl sghsaqdgaf qkllslatre ddtiywkdpa lavntsdkqs
     1141 dyffkpsfkd vemtayallt ltergdigna vpvmrwlvsk qnenggysst qdtvigiqal
     1201 arvaasvvsq tiaieasvky gdgrsrilki hsgnalvlqr lelpsdlkyv eieatgfgva
     1261 vlqvswsfnl avssespsff lnplldktst enylqlsvct hyrgegdasn mavmevalps
     1321 gylfdfdtls sihrtkevrr vesqdadtnv viyfdrigrq elcvtvpahr ehkvanqkpv
     1381 pvkvydyynl arsarmfysp hkatlcdice gvecgndcna vkqtksdatq leresepdga
     1441 agihashlsv avlaitvalf wr
//
LOCUS       XP_077503869            1462 aa            linear   INV 25-NOV-2025
DEFINITION  CD109 antigen-like isoform X5 [Amblyomma americanum].
ACCESSION   XP_077503869
VERSION     XP_077503869.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647743.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1462
                     /product="CD109 antigen-like isoform X5"
                     /calculated_mol_wt=161777
     Region          <104..1278
                     /region_name="YfaS"
                     /note="Uncharacterized conserved protein YfaS,
                     alpha-2-macroglobulin family [General function prediction
                     only]; COG2373"
                     /db_xref="CDD:441940"
     Region          909..1201
                     /region_name="A2M_2"
                     /note="Proteins similar to alpha2-macroglobulin (alpha
                     (2)-M). This group also contains the pregnancy zone
                     protein (PZP). Alpha(2)-M and PZP are broadly specific
                     proteinase inhibitors. Alpha (2)-M is a major carrier
                     protein in serum. The structural thioester...; cd02897"
                     /db_xref="CDD:239227"
     Site            order(923,925..926,929,969,979,982,1034,1036..1037,1187)
                     /site_type="other"
                     /note="surface patch"
                     /db_xref="CDD:239227"
     Site            923..926
                     /site_type="active"
                     /note="thioester region [active]"
                     /db_xref="CDD:239227"
     Site            order(1023,1027,1029,1037)
                     /site_type="other"
                     /note="specificity defining residues"
                     /db_xref="CDD:239227"
     Region          1308..1399
                     /region_name="A2M_recep"
                     /note="A-macroglobulin receptor binding domain; pfam07677"
                     /db_xref="CDD:462226"
     CDS             1..1462
                     /gene="LOC144114177"
                     /coded_by="XM_077647743.1:224..4612"
                     /db_xref="GeneID:144114177"
ORIGIN      
        1 mgaavlflla cvplaasqsg gvytivapqk lrpnlkyhvs aslsqsrspv dlqvtvsgra
       61 dsgnfntitk nallhdrdtq vlsfeigdwg pgkykitaqg sgglnfrnet dltyehksys
      121 vftqtdkavy kpgqkvlfrv ivvdpyllpt vtgamdvyia dakgnrihqw drvftqrgiy
      181 sselqlsdqp vlgdwtinvd vlgqkfrksf tvaeyvlptf evkvslpsya tynksevvat
      241 vsaaytygkp vkgtvtltvt prtryhqlnp rpyeqyqtkt tidgsvdipv aivrdlslkt
      301 dffsreieff alvteeltgr kynstsyitv hdkeikvelv ktsetfkpgl kytcflkvay
      361 qddspvndav nqlvlhygfn fneelwqssq hwvpqngvir lefyppnens tvilgfraef
      421 rgqtyyldgi ypakspsrsf lqafmvteep kvgdliefev natepldhlv yevvgrgdiv
      481 faqtlpvsgv rsyrfsfsat frmapkarvl vyyvrkdgev vadavnydvg gilrtpvhvq
      541 tsiaetkpgg qvnvlvstkp nayigvlgvd qsvlllkkgn dlsqeqviee lesfdsgkka
      601 klwppwyrrr rrslwwpgst tahdlfkdag mavltnglvy efddaiyark qvirldtdvl
      661 tnpvlppggl peappqvpgr irlrqqypet wlwtnatasp dgraviasti pdtitswvis
      721 afaldsltgl giapsqakvt vfrpffvtas lpysvlrges vaiqcvvfny nkkpveaevt
      781 lennrgefif sslsndvtge enkerrtkkv mvpaqdgipv sflitplklg yidirvsats
      841 slagdailkk llvkpegskq yfnkailidr rnpsapptia nisipipkna vpgserisis
      901 aigdllgpsv nnldqllvmp hgcgeqnmld fvpnvvvldy lkranrlspp vqnkairnle
      961 dgyqreltyk rddnsfsafg ntdksgstwl tafvlksfaq aapytvidpk vlenatawlv
     1021 arqqsdgsfn epgeiiykpm qsgagsgpal tayvlialle nkakqthpdv iqaaeayllk
     1081 elrttndpyv vslvtyalhl sghsaqdgaf qkllslatre ddtiywkdpa lavntsdkqs
     1141 dyffkpsfkd vemtayallt ltergdigna vpvmrwlvsk qnenggysst qdtvigiqal
     1201 arvaasvvsq tiaieasvky gdgrsrilki hsgnalvlqr lelpsdlkyv eieatgfgva
     1261 vlqvswsfnl avssespsff lnplldktst enylqlsvct hyrgegdasn mavmevalps
     1321 gylfdfdtls sihrtkevrr vesqdadtnv viyfdrigrq elcvtvpahr ehkvanqkpv
     1381 pvkvydyynl arsarmfysp hkatlcdice gvecgndcna vkqtksdatq leresepdga
     1441 agihashlsv avlaitvalf wr
//
LOCUS       XP_077503870            1462 aa            linear   INV 25-NOV-2025
DEFINITION  CD109 antigen-like isoform X5 [Amblyomma americanum].
ACCESSION   XP_077503870
VERSION     XP_077503870.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647744.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1462
                     /product="CD109 antigen-like isoform X5"
                     /calculated_mol_wt=161777
     Region          <104..1278
                     /region_name="YfaS"
                     /note="Uncharacterized conserved protein YfaS,
                     alpha-2-macroglobulin family [General function prediction
                     only]; COG2373"
                     /db_xref="CDD:441940"
     Region          909..1201
                     /region_name="A2M_2"
                     /note="Proteins similar to alpha2-macroglobulin (alpha
                     (2)-M). This group also contains the pregnancy zone
                     protein (PZP). Alpha(2)-M and PZP are broadly specific
                     proteinase inhibitors. Alpha (2)-M is a major carrier
                     protein in serum. The structural thioester...; cd02897"
                     /db_xref="CDD:239227"
     Site            order(923,925..926,929,969,979,982,1034,1036..1037,1187)
                     /site_type="other"
                     /note="surface patch"
                     /db_xref="CDD:239227"
     Site            923..926
                     /site_type="active"
                     /note="thioester region [active]"
                     /db_xref="CDD:239227"
     Site            order(1023,1027,1029,1037)
                     /site_type="other"
                     /note="specificity defining residues"
                     /db_xref="CDD:239227"
     Region          1308..1399
                     /region_name="A2M_recep"
                     /note="A-macroglobulin receptor binding domain; pfam07677"
                     /db_xref="CDD:462226"
     CDS             1..1462
                     /gene="LOC144114177"
                     /coded_by="XM_077647744.1:276..4664"
                     /db_xref="GeneID:144114177"
ORIGIN      
        1 mgaavlflla cvplaasqsg gvytivapqk lrpnlkyhvs aslsqsrspv dlqvtvsgra
       61 dsgnfntitk nallhdrdtq vlsfeigdwg pgkykitaqg sgglnfrnet dltyehksys
      121 vftqtdkavy kpgqkvlfrv ivvdpyllpt vtgamdvyia dakgnrihqw drvftqrgiy
      181 sselqlsdqp vlgdwtinvd vlgqkfrksf tvaeyvlptf evkvslpsya tynksevvat
      241 vsaaytygkp vkgtvtltvt prtryhqlnp rpyeqyqtkt tidgsvdipv aivrdlslkt
      301 dffsreieff alvteeltgr kynstsyitv hdkeikvelv ktsetfkpgl kytcflkvay
      361 qddspvndav nqlvlhygfn fneelwqssq hwvpqngvir lefyppnens tvilgfraef
      421 rgqtyyldgi ypakspsrsf lqafmvteep kvgdliefev natepldhlv yevvgrgdiv
      481 faqtlpvsgv rsyrfsfsat frmapkarvl vyyvrkdgev vadavnydvg gilrtpvhvq
      541 tsiaetkpgg qvnvlvstkp nayigvlgvd qsvlllkkgn dlsqeqviee lesfdsgkka
      601 klwppwyrrr rrslwwpgst tahdlfkdag mavltnglvy efddaiyark qvirldtdvl
      661 tnpvlppggl peappqvpgr irlrqqypet wlwtnatasp dgraviasti pdtitswvis
      721 afaldsltgl giapsqakvt vfrpffvtas lpysvlrges vaiqcvvfny nkkpveaevt
      781 lennrgefif sslsndvtge enkerrtkkv mvpaqdgipv sflitplklg yidirvsats
      841 slagdailkk llvkpegskq yfnkailidr rnpsapptia nisipipkna vpgserisis
      901 aigdllgpsv nnldqllvmp hgcgeqnmld fvpnvvvldy lkranrlspp vqnkairnle
      961 dgyqreltyk rddnsfsafg ntdksgstwl tafvlksfaq aapytvidpk vlenatawlv
     1021 arqqsdgsfn epgeiiykpm qsgagsgpal tayvlialle nkakqthpdv iqaaeayllk
     1081 elrttndpyv vslvtyalhl sghsaqdgaf qkllslatre ddtiywkdpa lavntsdkqs
     1141 dyffkpsfkd vemtayallt ltergdigna vpvmrwlvsk qnenggysst qdtvigiqal
     1201 arvaasvvsq tiaieasvky gdgrsrilki hsgnalvlqr lelpsdlkyv eieatgfgva
     1261 vlqvswsfnl avssespsff lnplldktst enylqlsvct hyrgegdasn mavmevalps
     1321 gylfdfdtls sihrtkevrr vesqdadtnv viyfdrigrq elcvtvpahr ehkvanqkpv
     1381 pvkvydyynl arsarmfysp hkatlcdice gvecgndcna vkqtksdatq leresepdga
     1441 agihashlsv avlaitvalf wr
//
LOCUS       XP_077503871            1462 aa            linear   INV 25-NOV-2025
DEFINITION  CD109 antigen-like isoform X5 [Amblyomma americanum].
ACCESSION   XP_077503871
VERSION     XP_077503871.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647745.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1462
                     /product="CD109 antigen-like isoform X5"
                     /calculated_mol_wt=161777
     Region          <104..1278
                     /region_name="YfaS"
                     /note="Uncharacterized conserved protein YfaS,
                     alpha-2-macroglobulin family [General function prediction
                     only]; COG2373"
                     /db_xref="CDD:441940"
     Region          909..1201
                     /region_name="A2M_2"
                     /note="Proteins similar to alpha2-macroglobulin (alpha
                     (2)-M). This group also contains the pregnancy zone
                     protein (PZP). Alpha(2)-M and PZP are broadly specific
                     proteinase inhibitors. Alpha (2)-M is a major carrier
                     protein in serum. The structural thioester...; cd02897"
                     /db_xref="CDD:239227"
     Site            order(923,925..926,929,969,979,982,1034,1036..1037,1187)
                     /site_type="other"
                     /note="surface patch"
                     /db_xref="CDD:239227"
     Site            923..926
                     /site_type="active"
                     /note="thioester region [active]"
                     /db_xref="CDD:239227"
     Site            order(1023,1027,1029,1037)
                     /site_type="other"
                     /note="specificity defining residues"
                     /db_xref="CDD:239227"
     Region          1308..1399
                     /region_name="A2M_recep"
                     /note="A-macroglobulin receptor binding domain; pfam07677"
                     /db_xref="CDD:462226"
     CDS             1..1462
                     /gene="LOC144114177"
                     /coded_by="XM_077647745.1:227..4615"
                     /db_xref="GeneID:144114177"
ORIGIN      
        1 mgaavlflla cvplaasqsg gvytivapqk lrpnlkyhvs aslsqsrspv dlqvtvsgra
       61 dsgnfntitk nallhdrdtq vlsfeigdwg pgkykitaqg sgglnfrnet dltyehksys
      121 vftqtdkavy kpgqkvlfrv ivvdpyllpt vtgamdvyia dakgnrihqw drvftqrgiy
      181 sselqlsdqp vlgdwtinvd vlgqkfrksf tvaeyvlptf evkvslpsya tynksevvat
      241 vsaaytygkp vkgtvtltvt prtryhqlnp rpyeqyqtkt tidgsvdipv aivrdlslkt
      301 dffsreieff alvteeltgr kynstsyitv hdkeikvelv ktsetfkpgl kytcflkvay
      361 qddspvndav nqlvlhygfn fneelwqssq hwvpqngvir lefyppnens tvilgfraef
      421 rgqtyyldgi ypakspsrsf lqafmvteep kvgdliefev natepldhlv yevvgrgdiv
      481 faqtlpvsgv rsyrfsfsat frmapkarvl vyyvrkdgev vadavnydvg gilrtpvhvq
      541 tsiaetkpgg qvnvlvstkp nayigvlgvd qsvlllkkgn dlsqeqviee lesfdsgkka
      601 klwppwyrrr rrslwwpgst tahdlfkdag mavltnglvy efddaiyark qvirldtdvl
      661 tnpvlppggl peappqvpgr irlrqqypet wlwtnatasp dgraviasti pdtitswvis
      721 afaldsltgl giapsqakvt vfrpffvtas lpysvlrges vaiqcvvfny nkkpveaevt
      781 lennrgefif sslsndvtge enkerrtkkv mvpaqdgipv sflitplklg yidirvsats
      841 slagdailkk llvkpegskq yfnkailidr rnpsapptia nisipipkna vpgserisis
      901 aigdllgpsv nnldqllvmp hgcgeqnmld fvpnvvvldy lkranrlspp vqnkairnle
      961 dgyqreltyk rddnsfsafg ntdksgstwl tafvlksfaq aapytvidpk vlenatawlv
     1021 arqqsdgsfn epgeiiykpm qsgagsgpal tayvlialle nkakqthpdv iqaaeayllk
     1081 elrttndpyv vslvtyalhl sghsaqdgaf qkllslatre ddtiywkdpa lavntsdkqs
     1141 dyffkpsfkd vemtayallt ltergdigna vpvmrwlvsk qnenggysst qdtvigiqal
     1201 arvaasvvsq tiaieasvky gdgrsrilki hsgnalvlqr lelpsdlkyv eieatgfgva
     1261 vlqvswsfnl avssespsff lnplldktst enylqlsvct hyrgegdasn mavmevalps
     1321 gylfdfdtls sihrtkevrr vesqdadtnv viyfdrigrq elcvtvpahr ehkvanqkpv
     1381 pvkvydyynl arsarmfysp hkatlcdice gvecgndcna vkqtksdatq leresepdga
     1441 agihashlsv avlaitvalf wr
//
LOCUS       XP_077503872            1456 aa            linear   INV 25-NOV-2025
DEFINITION  CD109 antigen-like isoform X6 [Amblyomma americanum].
ACCESSION   XP_077503872
VERSION     XP_077503872.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647746.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1456
                     /product="CD109 antigen-like isoform X6"
                     /calculated_mol_wt=161156
     Region          <104..1272
                     /region_name="YfaS"
                     /note="Uncharacterized conserved protein YfaS,
                     alpha-2-macroglobulin family [General function prediction
                     only]; COG2373"
                     /db_xref="CDD:441940"
     Region          903..1195
                     /region_name="A2M_2"
                     /note="Proteins similar to alpha2-macroglobulin (alpha
                     (2)-M). This group also contains the pregnancy zone
                     protein (PZP). Alpha(2)-M and PZP are broadly specific
                     proteinase inhibitors. Alpha (2)-M is a major carrier
                     protein in serum. The structural thioester...; cd02897"
                     /db_xref="CDD:239227"
     Site            order(917,919..920,923,963,973,976,1028,1030..1031,1181)
                     /site_type="other"
                     /note="surface patch"
                     /db_xref="CDD:239227"
     Site            917..920
                     /site_type="active"
                     /note="thioester region [active]"
                     /db_xref="CDD:239227"
     Site            order(1017,1021,1023,1031)
                     /site_type="other"
                     /note="specificity defining residues"
                     /db_xref="CDD:239227"
     Region          1302..1393
                     /region_name="A2M_recep"
                     /note="A-macroglobulin receptor binding domain; pfam07677"
                     /db_xref="CDD:462226"
     CDS             1..1456
                     /gene="LOC144114177"
                     /coded_by="XM_077647746.1:224..4594"
                     /db_xref="GeneID:144114177"
ORIGIN      
        1 mgaavlflla cvplaasqsg gvytivapqk lrpnlkyhvs aslsqsrspv dlqvtvsgra
       61 dsgnfntitk nallhdrdtq vlsfeigdwg pgkykitaqg sgglnfrnet dltyehksys
      121 vftqtdkavy kpgqkvlfrv ivvdpyllpt vtgamdvyia dakgnrihqw drvftqrgiy
      181 sselqlsdqp vlgdwtinvd vlgqkfrksf tvaeyvlptf evkvslpsya tynksevvat
      241 vsaaytygkp vkgtvtltvt prtryhqlnp rpyeqyqtkt tidgsvdipv aivrdlslkt
      301 dffsreieff alvteeltgr kynstsyitv hdkeikvelv ktsetfkpgl kytcflkvay
      361 qddspvndav nqlvlhygfn fneelwqssq hwvpqngvir lefyppnens tvilgfraef
      421 rgqtyyldgi ypakspsrsf lqafmvteep kvgdliefev natepldhlv yevvgrgdiv
      481 faqtlpvsgv rsyrfsfsat frmapkarvl vyyvrkdgev vadavnydvg gilrtpvhvq
      541 tsiaetkpgg qvnvlvstkp nayigvlgvd qsvlllkkgn dlsqeqviee lesfdsgkka
      601 klwppwyrrr rrslwwpgst tahdlfkdag mavltnglvy efddaiyyrs gfggefeldd
      661 saysggaagr negkhrlrrf fpetwlwnst ltgpdgravi astipdtits wvisafalds
      721 ltglgiapsq akvtvfrpff vtaslpysvl rgesvaiqcv vfnynkkpve aevtlennrg
      781 efifsslsnd vtgeenkerr tkkvmvpaqd gipvsflitp lklgyidirv satsslagda
      841 ilkkllvkpe gskqyfnkai lidrrnpsap ptianisipi pknavpgser isisaigdll
      901 gpsvnnldql lvmphgcgeq nmldfvpnvv vldylkranr lsppvqnkai rnledgyqre
      961 ltykrddnsf safgntdksg stwltafvlk sfaqaapytv idpkvlenat awlvarqqsd
     1021 gsfnepgeii ykpmqsgags gpaltayvli allenkakqt hpdviqaaea yllkelrttn
     1081 dpyvvslvty alhlsghsaq dgafqkllsl atreddtiyw kdpalavnts dkqsdyffkp
     1141 sfkdvemtay alltltergd ignavpvmrw lvskqnengg ysstqdtvig iqalarvaas
     1201 vvsqtiaiea svkygdgrsr ilkihsgnal vlqrlelpsd lkyveieatg fgvavlqvsw
     1261 sfnlavsses psfflnplld ktstenylql svcthyrgeg dasnmavmev alpsgylfdf
     1321 dtlssihrtk evrrvesqda dtnvviyfdr igrqelcvtv pahrehkvan qkpvpvkvyd
     1381 yynlarsarm fysphkatlc dicegvecgn dcnavkqtks datqlerese pdgaagihas
     1441 hlsvavlait valfwr
//
LOCUS       XP_077503873            1456 aa            linear   INV 25-NOV-2025
DEFINITION  CD109 antigen-like isoform X6 [Amblyomma americanum].
ACCESSION   XP_077503873
VERSION     XP_077503873.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647747.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1456
                     /product="CD109 antigen-like isoform X6"
                     /calculated_mol_wt=161156
     Region          <104..1272
                     /region_name="YfaS"
                     /note="Uncharacterized conserved protein YfaS,
                     alpha-2-macroglobulin family [General function prediction
                     only]; COG2373"
                     /db_xref="CDD:441940"
     Region          903..1195
                     /region_name="A2M_2"
                     /note="Proteins similar to alpha2-macroglobulin (alpha
                     (2)-M). This group also contains the pregnancy zone
                     protein (PZP). Alpha(2)-M and PZP are broadly specific
                     proteinase inhibitors. Alpha (2)-M is a major carrier
                     protein in serum. The structural thioester...; cd02897"
                     /db_xref="CDD:239227"
     Site            order(917,919..920,923,963,973,976,1028,1030..1031,1181)
                     /site_type="other"
                     /note="surface patch"
                     /db_xref="CDD:239227"
     Site            917..920
                     /site_type="active"
                     /note="thioester region [active]"
                     /db_xref="CDD:239227"
     Site            order(1017,1021,1023,1031)
                     /site_type="other"
                     /note="specificity defining residues"
                     /db_xref="CDD:239227"
     Region          1302..1393
                     /region_name="A2M_recep"
                     /note="A-macroglobulin receptor binding domain; pfam07677"
                     /db_xref="CDD:462226"
     CDS             1..1456
                     /gene="LOC144114177"
                     /coded_by="XM_077647747.1:251..4621"
                     /db_xref="GeneID:144114177"
ORIGIN      
        1 mgaavlflla cvplaasqsg gvytivapqk lrpnlkyhvs aslsqsrspv dlqvtvsgra
       61 dsgnfntitk nallhdrdtq vlsfeigdwg pgkykitaqg sgglnfrnet dltyehksys
      121 vftqtdkavy kpgqkvlfrv ivvdpyllpt vtgamdvyia dakgnrihqw drvftqrgiy
      181 sselqlsdqp vlgdwtinvd vlgqkfrksf tvaeyvlptf evkvslpsya tynksevvat
      241 vsaaytygkp vkgtvtltvt prtryhqlnp rpyeqyqtkt tidgsvdipv aivrdlslkt
      301 dffsreieff alvteeltgr kynstsyitv hdkeikvelv ktsetfkpgl kytcflkvay
      361 qddspvndav nqlvlhygfn fneelwqssq hwvpqngvir lefyppnens tvilgfraef
      421 rgqtyyldgi ypakspsrsf lqafmvteep kvgdliefev natepldhlv yevvgrgdiv
      481 faqtlpvsgv rsyrfsfsat frmapkarvl vyyvrkdgev vadavnydvg gilrtpvhvq
      541 tsiaetkpgg qvnvlvstkp nayigvlgvd qsvlllkkgn dlsqeqviee lesfdsgkka
      601 klwppwyrrr rrslwwpgst tahdlfkdag mavltnglvy efddaiyyrs gfggefeldd
      661 saysggaagr negkhrlrrf fpetwlwnst ltgpdgravi astipdtits wvisafalds
      721 ltglgiapsq akvtvfrpff vtaslpysvl rgesvaiqcv vfnynkkpve aevtlennrg
      781 efifsslsnd vtgeenkerr tkkvmvpaqd gipvsflitp lklgyidirv satsslagda
      841 ilkkllvkpe gskqyfnkai lidrrnpsap ptianisipi pknavpgser isisaigdll
      901 gpsvnnldql lvmphgcgeq nmldfvpnvv vldylkranr lsppvqnkai rnledgyqre
      961 ltykrddnsf safgntdksg stwltafvlk sfaqaapytv idpkvlenat awlvarqqsd
     1021 gsfnepgeii ykpmqsgags gpaltayvli allenkakqt hpdviqaaea yllkelrttn
     1081 dpyvvslvty alhlsghsaq dgafqkllsl atreddtiyw kdpalavnts dkqsdyffkp
     1141 sfkdvemtay alltltergd ignavpvmrw lvskqnengg ysstqdtvig iqalarvaas
     1201 vvsqtiaiea svkygdgrsr ilkihsgnal vlqrlelpsd lkyveieatg fgvavlqvsw
     1261 sfnlavsses psfflnplld ktstenylql svcthyrgeg dasnmavmev alpsgylfdf
     1321 dtlssihrtk evrrvesqda dtnvviyfdr igrqelcvtv pahrehkvan qkpvpvkvyd
     1381 yynlarsarm fysphkatlc dicegvecgn dcnavkqtks datqlerese pdgaagihas
     1441 hlsvavlait valfwr
//
LOCUS       XP_077503874            1456 aa            linear   INV 25-NOV-2025
DEFINITION  CD109 antigen-like isoform X6 [Amblyomma americanum].
ACCESSION   XP_077503874
VERSION     XP_077503874.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647748.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1456
                     /product="CD109 antigen-like isoform X6"
                     /calculated_mol_wt=161156
     Region          <104..1272
                     /region_name="YfaS"
                     /note="Uncharacterized conserved protein YfaS,
                     alpha-2-macroglobulin family [General function prediction
                     only]; COG2373"
                     /db_xref="CDD:441940"
     Region          903..1195
                     /region_name="A2M_2"
                     /note="Proteins similar to alpha2-macroglobulin (alpha
                     (2)-M). This group also contains the pregnancy zone
                     protein (PZP). Alpha(2)-M and PZP are broadly specific
                     proteinase inhibitors. Alpha (2)-M is a major carrier
                     protein in serum. The structural thioester...; cd02897"
                     /db_xref="CDD:239227"
     Site            order(917,919..920,923,963,973,976,1028,1030..1031,1181)
                     /site_type="other"
                     /note="surface patch"
                     /db_xref="CDD:239227"
     Site            917..920
                     /site_type="active"
                     /note="thioester region [active]"
                     /db_xref="CDD:239227"
     Site            order(1017,1021,1023,1031)
                     /site_type="other"
                     /note="specificity defining residues"
                     /db_xref="CDD:239227"
     Region          1302..1393
                     /region_name="A2M_recep"
                     /note="A-macroglobulin receptor binding domain; pfam07677"
                     /db_xref="CDD:462226"
     CDS             1..1456
                     /gene="LOC144114177"
                     /coded_by="XM_077647748.1:276..4646"
                     /db_xref="GeneID:144114177"
ORIGIN      
        1 mgaavlflla cvplaasqsg gvytivapqk lrpnlkyhvs aslsqsrspv dlqvtvsgra
       61 dsgnfntitk nallhdrdtq vlsfeigdwg pgkykitaqg sgglnfrnet dltyehksys
      121 vftqtdkavy kpgqkvlfrv ivvdpyllpt vtgamdvyia dakgnrihqw drvftqrgiy
      181 sselqlsdqp vlgdwtinvd vlgqkfrksf tvaeyvlptf evkvslpsya tynksevvat
      241 vsaaytygkp vkgtvtltvt prtryhqlnp rpyeqyqtkt tidgsvdipv aivrdlslkt
      301 dffsreieff alvteeltgr kynstsyitv hdkeikvelv ktsetfkpgl kytcflkvay
      361 qddspvndav nqlvlhygfn fneelwqssq hwvpqngvir lefyppnens tvilgfraef
      421 rgqtyyldgi ypakspsrsf lqafmvteep kvgdliefev natepldhlv yevvgrgdiv
      481 faqtlpvsgv rsyrfsfsat frmapkarvl vyyvrkdgev vadavnydvg gilrtpvhvq
      541 tsiaetkpgg qvnvlvstkp nayigvlgvd qsvlllkkgn dlsqeqviee lesfdsgkka
      601 klwppwyrrr rrslwwpgst tahdlfkdag mavltnglvy efddaiyyrs gfggefeldd
      661 saysggaagr negkhrlrrf fpetwlwnst ltgpdgravi astipdtits wvisafalds
      721 ltglgiapsq akvtvfrpff vtaslpysvl rgesvaiqcv vfnynkkpve aevtlennrg
      781 efifsslsnd vtgeenkerr tkkvmvpaqd gipvsflitp lklgyidirv satsslagda
      841 ilkkllvkpe gskqyfnkai lidrrnpsap ptianisipi pknavpgser isisaigdll
      901 gpsvnnldql lvmphgcgeq nmldfvpnvv vldylkranr lsppvqnkai rnledgyqre
      961 ltykrddnsf safgntdksg stwltafvlk sfaqaapytv idpkvlenat awlvarqqsd
     1021 gsfnepgeii ykpmqsgags gpaltayvli allenkakqt hpdviqaaea yllkelrttn
     1081 dpyvvslvty alhlsghsaq dgafqkllsl atreddtiyw kdpalavnts dkqsdyffkp
     1141 sfkdvemtay alltltergd ignavpvmrw lvskqnengg ysstqdtvig iqalarvaas
     1201 vvsqtiaiea svkygdgrsr ilkihsgnal vlqrlelpsd lkyveieatg fgvavlqvsw
     1261 sfnlavsses psfflnplld ktstenylql svcthyrgeg dasnmavmev alpsgylfdf
     1321 dtlssihrtk evrrvesqda dtnvviyfdr igrqelcvtv pahrehkvan qkpvpvkvyd
     1381 yynlarsarm fysphkatlc dicegvecgn dcnavkqtks datqlerese pdgaagihas
     1441 hlsvavlait valfwr
//
LOCUS       XP_077503875            1456 aa            linear   INV 25-NOV-2025
DEFINITION  CD109 antigen-like isoform X6 [Amblyomma americanum].
ACCESSION   XP_077503875
VERSION     XP_077503875.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647749.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1456
                     /product="CD109 antigen-like isoform X6"
                     /calculated_mol_wt=161156
     Region          <104..1272
                     /region_name="YfaS"
                     /note="Uncharacterized conserved protein YfaS,
                     alpha-2-macroglobulin family [General function prediction
                     only]; COG2373"
                     /db_xref="CDD:441940"
     Region          903..1195
                     /region_name="A2M_2"
                     /note="Proteins similar to alpha2-macroglobulin (alpha
                     (2)-M). This group also contains the pregnancy zone
                     protein (PZP). Alpha(2)-M and PZP are broadly specific
                     proteinase inhibitors. Alpha (2)-M is a major carrier
                     protein in serum. The structural thioester...; cd02897"
                     /db_xref="CDD:239227"
     Site            order(917,919..920,923,963,973,976,1028,1030..1031,1181)
                     /site_type="other"
                     /note="surface patch"
                     /db_xref="CDD:239227"
     Site            917..920
                     /site_type="active"
                     /note="thioester region [active]"
                     /db_xref="CDD:239227"
     Site            order(1017,1021,1023,1031)
                     /site_type="other"
                     /note="specificity defining residues"
                     /db_xref="CDD:239227"
     Region          1302..1393
                     /region_name="A2M_recep"
                     /note="A-macroglobulin receptor binding domain; pfam07677"
                     /db_xref="CDD:462226"
     CDS             1..1456
                     /gene="LOC144114177"
                     /coded_by="XM_077647749.1:197..4567"
                     /db_xref="GeneID:144114177"
ORIGIN      
        1 mgaavlflla cvplaasqsg gvytivapqk lrpnlkyhvs aslsqsrspv dlqvtvsgra
       61 dsgnfntitk nallhdrdtq vlsfeigdwg pgkykitaqg sgglnfrnet dltyehksys
      121 vftqtdkavy kpgqkvlfrv ivvdpyllpt vtgamdvyia dakgnrihqw drvftqrgiy
      181 sselqlsdqp vlgdwtinvd vlgqkfrksf tvaeyvlptf evkvslpsya tynksevvat
      241 vsaaytygkp vkgtvtltvt prtryhqlnp rpyeqyqtkt tidgsvdipv aivrdlslkt
      301 dffsreieff alvteeltgr kynstsyitv hdkeikvelv ktsetfkpgl kytcflkvay
      361 qddspvndav nqlvlhygfn fneelwqssq hwvpqngvir lefyppnens tvilgfraef
      421 rgqtyyldgi ypakspsrsf lqafmvteep kvgdliefev natepldhlv yevvgrgdiv
      481 faqtlpvsgv rsyrfsfsat frmapkarvl vyyvrkdgev vadavnydvg gilrtpvhvq
      541 tsiaetkpgg qvnvlvstkp nayigvlgvd qsvlllkkgn dlsqeqviee lesfdsgkka
      601 klwppwyrrr rrslwwpgst tahdlfkdag mavltnglvy efddaiyyrs gfggefeldd
      661 saysggaagr negkhrlrrf fpetwlwnst ltgpdgravi astipdtits wvisafalds
      721 ltglgiapsq akvtvfrpff vtaslpysvl rgesvaiqcv vfnynkkpve aevtlennrg
      781 efifsslsnd vtgeenkerr tkkvmvpaqd gipvsflitp lklgyidirv satsslagda
      841 ilkkllvkpe gskqyfnkai lidrrnpsap ptianisipi pknavpgser isisaigdll
      901 gpsvnnldql lvmphgcgeq nmldfvpnvv vldylkranr lsppvqnkai rnledgyqre
      961 ltykrddnsf safgntdksg stwltafvlk sfaqaapytv idpkvlenat awlvarqqsd
     1021 gsfnepgeii ykpmqsgags gpaltayvli allenkakqt hpdviqaaea yllkelrttn
     1081 dpyvvslvty alhlsghsaq dgafqkllsl atreddtiyw kdpalavnts dkqsdyffkp
     1141 sfkdvemtay alltltergd ignavpvmrw lvskqnengg ysstqdtvig iqalarvaas
     1201 vvsqtiaiea svkygdgrsr ilkihsgnal vlqrlelpsd lkyveieatg fgvavlqvsw
     1261 sfnlavsses psfflnplld ktstenylql svcthyrgeg dasnmavmev alpsgylfdf
     1321 dtlssihrtk evrrvesqda dtnvviyfdr igrqelcvtv pahrehkvan qkpvpvkvyd
     1381 yynlarsarm fysphkatlc dicegvecgn dcnavkqtks datqlerese pdgaagihas
     1441 hlsvavlait valfwr
//
LOCUS       XP_077503876            7453 aa            linear   INV 25-NOV-2025
DEFINITION  MAM and LDL-receptor class A domain-containing protein 2-like
            [Amblyomma americanum].
ACCESSION   XP_077503876
VERSION     XP_077503876.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647750.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7453
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..7453
                     /product="MAM and LDL-receptor class A domain-containing
                     protein 2-like"
                     /calculated_mol_wt=822896
     Region          34..191
                     /region_name="MAM"
                     /note="MAM domain, meprin/A5/mu; pfam00629"
                     /db_xref="CDD:459878"
     Region          199..372
                     /region_name="MAM"
                     /note="MAM domain, meprin/A5/mu; pfam00629"
                     /db_xref="CDD:459878"
     Region          381..535
                     /region_name="MAM"
                     /note="Meprin, A5 protein, and protein tyrosine
                     phosphatase Mu (MAM) domain. MAM is an extracellular
                     domain which mediates protein-protein interactions and is
                     found in a diverse set of proteins, many of which are
                     known to function in cell adhesion. Members...; cd06263"
                     /db_xref="CDD:99706"
     Region          544..707
                     /region_name="MAM"
                     /note="MAM domain, meprin/A5/mu; pfam00629"
                     /db_xref="CDD:459878"
     Region          733..883
                     /region_name="MAM"
                     /note="Meprin, A5 protein, and protein tyrosine
                     phosphatase Mu (MAM) domain. MAM is an extracellular
                     domain which mediates protein-protein interactions and is
                     found in a diverse set of proteins, many of which are
                     known to function in cell adhesion. Members...; cd06263"
                     /db_xref="CDD:99706"
     Region          893..1045
                     /region_name="MAM"
                     /note="Meprin, A5 protein, and protein tyrosine
                     phosphatase Mu (MAM) domain. MAM is an extracellular
                     domain which mediates protein-protein interactions and is
                     found in a diverse set of proteins, many of which are
                     known to function in cell adhesion. Members...; cd06263"
                     /db_xref="CDD:99706"
     Region          1054..1219
                     /region_name="MAM"
                     /note="Meprin, A5 protein, and protein tyrosine
                     phosphatase Mu (MAM) domain. MAM is an extracellular
                     domain which mediates protein-protein interactions and is
                     found in a diverse set of proteins, many of which are
                     known to function in cell adhesion. Members...; cd06263"
                     /db_xref="CDD:99706"
     Region          1237..1394
                     /region_name="MAM"
                     /note="MAM domain, meprin/A5/mu; pfam00629"
                     /db_xref="CDD:459878"
     Region          1399..1555
                     /region_name="MAM"
                     /note="MAM domain, meprin/A5/mu; pfam00629"
                     /db_xref="CDD:459878"
     Region          1560..1696
                     /region_name="MAM"
                     /note="Meprin, A5 protein, and protein tyrosine
                     phosphatase Mu (MAM) domain. MAM is an extracellular
                     domain which mediates protein-protein interactions and is
                     found in a diverse set of proteins, many of which are
                     known to function in cell adhesion. Members...; cd06263"
                     /db_xref="CDD:99706"
     Region          1707..1737
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1707,1716,1727..1728)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1720,1723,1727,1733..1734)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1730..1734
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1740..1901
                     /region_name="MAM"
                     /note="Meprin, A5 protein, and protein tyrosine
                     phosphatase Mu (MAM) domain. MAM is an extracellular
                     domain which mediates protein-protein interactions and is
                     found in a diverse set of proteins, many of which are
                     known to function in cell adhesion. Members...; cd06263"
                     /db_xref="CDD:99706"
     Region          1915..1947
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1918,1926,1937..1938)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1930,1933,1937,1943..1944)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1940..1944
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1954..2108
                     /region_name="MAM"
                     /note="MAM domain, meprin/A5/mu; pfam00629"
                     /db_xref="CDD:459878"
     Region          2132..2167
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(2137,2146,2157..2158)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2150,2153,2157,2163..2164)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2160..2164
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2347..2501
                     /region_name="MAM"
                     /note="MAM domain, meprin/A5/mu; pfam00629"
                     /db_xref="CDD:459878"
     Region          2506..2655
                     /region_name="MAM"
                     /note="Meprin, A5 protein, and protein tyrosine
                     phosphatase Mu (MAM) domain. MAM is an extracellular
                     domain which mediates protein-protein interactions and is
                     found in a diverse set of proteins, many of which are
                     known to function in cell adhesion. Members...; cd06263"
                     /db_xref="CDD:99706"
     Region          2661..2819
                     /region_name="MAM"
                     /note="MAM domain, meprin/A5/mu; pfam00629"
                     /db_xref="CDD:459878"
     Region          2839..>2962
                     /region_name="MAM"
                     /note="MAM domain, meprin/A5/mu; pfam00629"
                     /db_xref="CDD:459878"
     Region          3004..3168
                     /region_name="MAM"
                     /note="MAM domain, meprin/A5/mu; pfam00629"
                     /db_xref="CDD:459878"
     Region          3173..3343
                     /region_name="MAM"
                     /note="MAM domain, meprin/A5/mu; pfam00629"
                     /db_xref="CDD:459878"
     Region          3386..3540
                     /region_name="MAM"
                     /note="Meprin, A5 protein, and protein tyrosine
                     phosphatase Mu (MAM) domain. MAM is an extracellular
                     domain which mediates protein-protein interactions and is
                     found in a diverse set of proteins, many of which are
                     known to function in cell adhesion. Members...; cd06263"
                     /db_xref="CDD:99706"
     Region          3551..3584
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(3557,3566,3577..3578)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3570,3573,3577,3583..3584)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3580..3584
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3595..3763
                     /region_name="MAM"
                     /note="MAM domain, meprin/A5/mu; pfam00629"
                     /db_xref="CDD:459878"
     Region          3782..3816
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3787,3795,3806..3807)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3799,3802,3806,3812..3813)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3809..3813
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3819..3972
                     /region_name="MAM"
                     /note="MAM domain, meprin/A5/mu; pfam00629"
                     /db_xref="CDD:459878"
     Region          3975..4139
                     /region_name="MAM"
                     /note="Domain in meprin, A5, receptor protein tyrosine
                     phosphatase mu (and others); smart00137"
                     /db_xref="CDD:214533"
     Region          4145..4305
                     /region_name="MAM"
                     /note="Meprin, A5 protein, and protein tyrosine
                     phosphatase Mu (MAM) domain. MAM is an extracellular
                     domain which mediates protein-protein interactions and is
                     found in a diverse set of proteins, many of which are
                     known to function in cell adhesion. Members...; cd06263"
                     /db_xref="CDD:99706"
     Region          4311..4475
                     /region_name="MAM"
                     /note="Meprin, A5 protein, and protein tyrosine
                     phosphatase Mu (MAM) domain. MAM is an extracellular
                     domain which mediates protein-protein interactions and is
                     found in a diverse set of proteins, many of which are
                     known to function in cell adhesion. Members...; cd06263"
                     /db_xref="CDD:99706"
     Region          4498..4664
                     /region_name="MAM"
                     /note="MAM domain, meprin/A5/mu; pfam00629"
                     /db_xref="CDD:459878"
     Region          4669..4829
                     /region_name="MAM"
                     /note="Meprin, A5 protein, and protein tyrosine
                     phosphatase Mu (MAM) domain. MAM is an extracellular
                     domain which mediates protein-protein interactions and is
                     found in a diverse set of proteins, many of which are
                     known to function in cell adhesion. Members...; cd06263"
                     /db_xref="CDD:99706"
     Region          4829..4985
                     /region_name="MAM"
                     /note="Meprin, A5 protein, and protein tyrosine
                     phosphatase Mu (MAM) domain. MAM is an extracellular
                     domain which mediates protein-protein interactions and is
                     found in a diverse set of proteins, many of which are
                     known to function in cell adhesion. Members...; cd06263"
                     /db_xref="CDD:99706"
     Region          5030..5193
                     /region_name="MAM"
                     /note="MAM domain, meprin/A5/mu; pfam00629"
                     /db_xref="CDD:459878"
     Region          5204..5235
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(5209,5217,5228..5229)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(5221,5224,5228,5234..5235)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            5231..5235
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          5250..5417
                     /region_name="MAM"
                     /note="MAM domain, meprin/A5/mu; pfam00629"
                     /db_xref="CDD:459878"
     Region          5442..5476
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(5447,5455,5466..5467)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(5459,5462,5466,5472..5473)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            5469..5473
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          5479..5632
                     /region_name="MAM"
                     /note="Meprin, A5 protein, and protein tyrosine
                     phosphatase Mu (MAM) domain. MAM is an extracellular
                     domain which mediates protein-protein interactions and is
                     found in a diverse set of proteins, many of which are
                     known to function in cell adhesion. Members...; cd06263"
                     /db_xref="CDD:99706"
     Region          5644..5679
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(5648,5658,5669..5670)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(5662,5665,5669,5675..5676)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            5672..5676
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          5686..5839
                     /region_name="MAM"
                     /note="MAM domain, meprin/A5/mu; pfam00629"
                     /db_xref="CDD:459878"
     Region          5858..5891
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(5862,5870,5881..5882)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(5874,5877,5881,5887..5888)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            5884..5888
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          5895..6057
                     /region_name="MAM"
                     /note="MAM domain, meprin/A5/mu; pfam00629"
                     /db_xref="CDD:459878"
     Region          6069..6105
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(6075,6084,6095..6096)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(6088,6091,6095,6101..6102)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            6098..6102
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          6129..6274
                     /region_name="MAM"
                     /note="MAM domain, meprin/A5/mu; pfam00629"
                     /db_xref="CDD:459878"
     Region          6300..6335
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(6305,6314,6325..6326)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(6318,6321,6325,6331..6332)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            6328..6332
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          6338..6517
                     /region_name="MAM"
                     /note="Domain in meprin, A5, receptor protein tyrosine
                     phosphatase mu (and others); smart00137"
                     /db_xref="CDD:214533"
     Region          6529..6562
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(6535,6544,6555..6556)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(6548,6551,6555,6561..6562)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            6558..6562
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          6574..6739
                     /region_name="MAM"
                     /note="MAM domain, meprin/A5/mu; pfam00629"
                     /db_xref="CDD:459878"
     Region          6760..6794
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(6765,6773,6784..6785)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(6777,6780,6784,6790..6791)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            6787..6791
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          6798..6948
                     /region_name="MAM"
                     /note="Meprin, A5 protein, and protein tyrosine
                     phosphatase Mu (MAM) domain. MAM is an extracellular
                     domain which mediates protein-protein interactions and is
                     found in a diverse set of proteins, many of which are
                     known to function in cell adhesion. Members...; cd06263"
                     /db_xref="CDD:99706"
     Region          6959..6994
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(6964,6973,6984..6985)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(6977,6980,6984,6990..6991)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            6987..6991
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          7001..7157
                     /region_name="MAM"
                     /note="MAM domain, meprin/A5/mu; pfam00629"
                     /db_xref="CDD:459878"
     Region          7174..7209
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(7179,7188,7199..7200)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(7192,7195,7199,7205..7206)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            7202..7206
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          7244..7280
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(7249,7259,7270..7271)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(7263,7266,7270,7276..7277)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            7273..7277
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          7286..7321
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(7291,7300,7311..7312)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(7304,7307,7311,7317..7318)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            7314..7318
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     CDS             1..7453
                     /gene="LOC144114179"
                     /coded_by="XM_077647750.1:85..22446"
                     /db_xref="GeneID:144114179"
ORIGIN      
        1 mgrppgsill llllvhdils thsatgdpgk savcdfepgn cagwtptdcs kgypcwkavk
       61 asevsegpvs dhstgtgggk ygramfpgrq rrsgtavmkr nargpfcfta wlhisalslp
      121 nvifsavyrp kyffgqqerv ffagrmselq lwqkvvyeen rggnieikie ssytdgtvav
      181 ddisitpgkc pdpprdgsct fdtstcgytn hesngkhkwi ranmrnsral nwprrdhttg
      241 tpsggyalie mpyreissgi ltsrvlskpe spsqclrfly fvpdasgnrg llvrllrnpl
      301 pasrtqgnat aaegdetlwh ldaallpnrw tpvevsytvr dnyelhfrcy ssttrspdff
      361 caiddveifp cddkkgekms csfddghlck wqtsnhtgdv pwelsdveng vpqlpredht
      421 lstpqgkfvy lenkasskvv kakllspald pkwvgaacls fwhfavledp rscnltvlsa
      481 nesewwsssh qlkrswsqev vrlwlqegkg qvsieaslgm alialddvvl tygqcaserr
      541 glscdfergp ctwtnamskr gtsewfvrgg hlrstlprpa tdhtlgtsqg syvfvsgagl
      601 ysaqsatlvs eyvnlavpgv qcmdfwfivq nvhgtqlsvf meqrgysdfn rdvvwsagps
      661 nftgwrrgqv kvprrarvml dgkvvpnnkn syiaiddisi evnsncrslp pgaaagneay
      721 vlldcswsgp rscpwwatss fdvlknsrst ppeyflapas gpeghrgsfa ytgcdsrssp
      781 qkttaflqtq lvrstrdpvc lrfslhafsg igwqvklnqv igtkdnvlfl sagrttadrw
      841 yrvartvsfn tpqasklvfi fnnnlcqsgg valgdihatp gpcssidtek glcdfesgtc
      901 nwdargawkr vrvtsdkrls dasilsgppf lgycllldst nsmgvkgsla spvwsarhqp
      961 rllefwyird rsrsanlwve lqtpsgasia viwqlpttap gltwnlgrve ipvrdedfkv
     1021 aiqgtsgrts nyrtllavdd vrlstrsgsh vancnfegdl cgyasvagsd pklrwfvgsg
     1081 rvrkpslkpt vpalplvgls eglqasktfa yvdttvplqs nskisnatlr savftarend
     1141 tlllryfrkg natesflvyq skwtkgedki twvtlgslqd gddwqdfeap lqaadesqlh
     1201 vvitrssnql gfaavasisv gharaivrek peqtvdcnfe natfcnwkpd stggsqlewk
     1261 lndpqsrqpa fpsfdsttrs ykgkyiyaes nksagteaar lkspavptew lksmcfsfwy
     1321 fsvadvnssl svslssasda awhssvsrlt awthgqtqfe esapqegini ileasiqngl
     1381 vavddlsatp glcsatqlcs feggvdcsle sdidnvrdwt vvdgsglgvr dhstdstagh
     1441 flylnttyve qklhsfgrif lperdateat cltfwwravg adsvfnvyny rrgvglrdpv
     1501 lsfvtrenpw wnarsitlss ektwqavfev llpelalqes siflddieli sgfcrpenyc
     1561 sfetptclhw knsdedstsh wsleraglsv itrdhtlqsk eghyllfrgd rpqkraslvl
     1621 tdrryhcatm whyiseapan ltitagdvrf akstggqwke amfpvdikna vvsaiagghs
     1681 dafaaiddll lieercyqqp kpapqpfvca asgqkvpaaa rcnfiadcsd gsdevdcgac
     1741 tfengtcgws vegrgllrsd dfswqrqkaa tpvggplrdh ttsssrghyl llrakqpieg
     1801 mtvigeassp vlhntghlcv vkfwfnyras ndthvfllel hvngfqivaw dswelaenps
     1861 egkwnegqfl lgryrstvqi ylrgyeaprd dsytaiddis fegcdmpvps veqnpgmftc
     1921 knkvsivmeh lcdytdhcgd fsdeedcedf rfrcsfdssf cdwtpavsar gggwtrskpf
     1981 kyltrgptrd htsggkdgsf lylkseaksv sasllgpvfn attlcvmrfy fvvkastnav
     2041 lsvktrnnad gefkevwrsd qptefrhfte afvafreasd fqvsiegsle gdrdaqgyia
     2101 iddvtflnsc haasgllpks spdtstpaps nctnghfacl sgdrcipasq lcdflkqcpd
     2161 gsdeshcgac dfstdlcgle gahsqsqprw srvsalmvan nrarfpslpr edsghsergf
     2221 yvtylktdga vpktdrntal mtpplgamgh acrltfysfl pkhtktlhls vavversrdq
     2281 knkiikksvl largdedqaa wemqaanign rlsgariyys gsenvsiddi eyhhchpdda
     2341 redsvnctfe ekegcgwype nvadsgdwkv isgapffava dhttgqgmyl alenfegttv
     2401 vahmvsarln vtgdagrcfr mwyhmhgdrn srlnlrlfnv sntdsnlwtr sghqgdtwme
     2461 avinikvtda pfqlllegel psyspsfigv ddisfderpc pptltcnfee gpcdwtlegw
     2521 kldtggsfka vptdhtagts tghfarlerr kgrilsakhd lsvaerrcfr fwhfftgqre
     2581 qtlsvykhta esaetpelvw aihgedrpph qwlsgsvnva vnessslrle lqgenpegnv
     2641 salavddlll sgwacprpgs csfeddlcdw shvagssdla wqrwsgptpd rsgpaidhtl
     2701 gsrhghflfl saeragdkrv giiesellhy sprsclqfyy fvdpkgsqan lsvqyarsgk
     2761 vfrrigvlak grsagwqlfr slqswlpaay nirisgypgn depvdvaidd ievfdrdcpg
     2821 nmpaddtpve trnstfwdcd fdmdfcswsq sgntwiirsg rtailtgdgp htdsklrtle
     2881 gkyayfspqp sspsdmltsa alapsrndyc vqfwyflysl aptqlviglv neasssvdqv
     2941 swaqqrgfdr iwrrgsfivp sggaggkrla vkvqqrhlgl aeialdqmrv apsglcthpa
     3001 gtlcdfegdg ycgflvdssg ggrwriaggn sdpvtlssqy sdhtfgasqg ghflllhlse
     3061 qkgkqdapyv aslvvpdqap mlercltlwf fvegtgvanl nvsvraqgst esvlqdmlfg
     3121 apstgwrygs atvrsaswhe vlltaevehg gtaavglddi slsdgscpeq ghcdfegpdm
     3181 cgwenaesgv qrmwarnrgs iprsllgpra dhtlgtpdgt yvyldgrnts gegtytgilk
     3241 sqlwrgssay cfsfwlymrg tvsgpstssl rvivaydqne kekrdalavf tgsqgkrwtr
     3301 krvtlkppas afgefrvlla gttgvpgggd iavddveviq gacdpnlevd kfkchdgvtf
     3361 vnisqvcdfk kdcpgsgdde qlcgqcdfdv dtcgwyskdd wkwtiakdne hnsslpmkdq
     3421 srgdehggyv yssgrgylve ggklfhspnn thrlkrsgkd ctlsfwyfas grsnqlsvhk
     3481 ytlgydatlw lvekdglqvw tkeratigrs ksefdlafea ssgfsaadtf vaidtisfen
     3541 calpapspkn csgpnrfscp etnvcisknq lcdhvddcgd gsdevldicq nyticsyepr
     3601 sdpctwkaas aeserkslew rvsyvpyrkg dpntgpytdh tmgghgsgrv lllrplrrdd
     3661 lnssafyrsp nyiasngecs ltfhyylhgq dvggialfaq yenytekpqw knlfeakgqr
     3721 gqrwilarav vqddkpfcfi iegslgagya sdiaiddisv spgcisyndt lpnpapppmp
     3781 kckasqyacr nggcipnqlv cdfeedcadg sdeemcgpcs feygtcgwtd vssgkvtwqv
     3841 kqaryvegpk idhttksaag hllalnndey fastavvlsp qlppsssrcs msfwyyysev
     3901 fnaaamdvry llpqnysvkl vevapakawt klvvniprrd trdariafya nvtelyrmss
     3961 igiddisfmn crpsslfidc sfdnktnnng lchwqntgks sngwqvkfpg pnhdgvgpta
     4021 dhtsgrggya ifsaedatqt aslesaetqp saknvscltf wysmnngnsl sllieaktmk
     4081 nssvkftret aartgwnfge lpvslqepyt vtisaikakk aaagafiamd divltegpcr
     4141 rtgfcdfetd fcdwklakig lgfgwrrisp atetatcpah dhtlgtsdgh yavvthkrqg
     4201 dkatlsspvf entgnrclry wfnmagnsig tlsvyqrlng ssriedlkpv wkrsgdhnga
     4261 wrrghvklrn lrrfqvviea faeatnaegs syvalddlef slepcedtvt cnfetdtcgw
     4321 ytdgtssnip wirttgsagv ylggpgvdvt tssahgnyll aefgnrselt emvllsdyvd
     4381 vqhqssycfs lwyisrngse ssllvksvkd gdarsaetlg nltqrttwtk qaitvlteee
     4441 yqrsfqival pgrnsdlstp pgvavdevgf fygscdggtk ppasnetalr ypphpldcdf
     4501 edddcawang stssrsvwvr kfaeghgdve lvlpetdhtt ksvhgsfayl gltstagsqr
     4561 pfkediaile sefplhvsdg gaclkfwffa fgsdvaplvl kerdavtras rvawtnawsr
     4621 gpqwnyaqvh legdkavflg fessktpggh talddisvnt gqcprspycd feaddcgwrv
     4681 rdgteqlrwe rmsassttgg sdhtlgsadg hvlavntssp daykgmtvtt fsqtqeagss
     4741 scvrfwyqls sghklsvgti egamerpaat ftqsalissg gwhpaqvnik stskkpfeyy
     4801 lrvtlnakgg iaavddfqil nacpslgscd feedfcfwen vptsagsalw drftgrshmf
     4861 gpdvdhtfse sfgtyavigs nanlidipal lvspllsdcn nicfsfwsyh igtlphmlnl
     4921 flrtkfgdda ieysftadvn arwikaqani stdasecrig frvgfppfqp svlalddlvv
     4981 sggacehled inhpefscds etkhltrdkl cnfevdcsdg kdekncgiec dfeskgtcnw
     5041 krtsagalws lekpknstir eptedhttrs eegsflrfkv qaeqeherta sffspnlida
     5101 shtctlgfwy ystvlqqstv irvvyalssk lklvdttalv lsghngtler kkwtyaaari
     5161 grvserfsvk figklesfde tfliddlrfs ecnrkivark stycpphfft cangncipks
     5221 tvcdfnddcg dmsdeglspr anctffparc dfeegsvcdw qvhsptwelr ltgaqeyglt
     5281 nddlrdhttn svygnflrfl fvdrslekge lsspiiraae garcflrffy ayqttfshld
     5341 yrlysinags lavyvrldll gekhlvwkts rvfgqhyerk vislekfsgp mqvvieasag
     5401 kdtsgawald dlsftdgcsv splttlpvlr peattespsr scsseefacp dntciplnqv
     5461 cnfvadckgg adeaacascn fdsgtcgwqd vsigryswhr lqggqdgsdp stdvsgkgff
     5521 mgvtagegvv ldnavlksve hgasaascyl emfyyannvv sgaeilrlsi lkrnsssetp
     5581 llalrtveiq kwkkvivkig rqayggwalk lvatpptsls riavddislh dcgqssilgs
     5641 dceskgqfsc keshdicytk sqlcdwsldc phgsdelncs tfpercsfen gmcgwaegvv
     5701 eghsrwsivr gealaddegp nfdhtfgnds ghylflkkds sqkshatlks vtfkstttre
     5761 crlrywymia gnakveviiq pearehelhk lkelsgndti rewqkidlil sspkdfqvvl
     5821 dgwpdgyiai ddvsftpdcl vshssivptl spagkcksdt eftckdnsci lkdmvcdfks
     5881 dcpfgsdese cpsscsfeqg ecgwnslnlp estawrsmta meareqvkns pavdvtknss
     5941 sgsylsfyva hdgvrlespv rmfsqrfqqs tpackinfyh wltlpvvhvr llivsddsee
     6001 illwertskk lakswifqsi gvgrrkghfr lafevdspss ggglhfalde infvdcgypr
     6061 ddelsgdpcs qkdsfkcksr qfcissekkc dlyddcgdss deedcvnerv tfddeqsgvl
     6121 evgkpkrddt egslqlqrgr slsrkhddtg plfdhttfnk sgaylqfgga yhgfnkmatl
     6181 ssltlqsgsc rvtfhsymfg rqvnllsvym ryfnnssdak evwhqegpag dfwkrhrvdv
     6241 sdtrdrqivf avrsgkgped vialddisfs qecrfsaksl pedpsaasts tpaapepgrc
     6301 dqqqftcisd glcvswdrvc dfrkdctdgs dekncvqpsc gfengilcgw avtsnaslla
     6361 sasgttrftt yslenefrwk liqandqsdr anrelrpqid hsegtqqgwy alansayggt
     6421 teasllyssr plshtaggcr lqawyyctdd ctlqllryss tdgavqerpl wtafrgrske
     6481 wtlvqvpigi lrearlawra qrgynqqavq alddlefisc eppelrnatc ppdqfrcvld
     6541 nwciersqlc dqtrdcedgs denavacstm rsrcsfedah cedwtndaer girlfwsrri
     6601 agaadpnlpm tdhttssgqg aymavykrwv gttgvttsgr lssyvintnp kiacqvrfwy
     6661 nvhqrgvsle vyrqtnlgdg glshvrtlpv tgpdywnrad infgtanetf rvvikavftd
     6721 sgalqgaavd ditmtdgcrr sdrelpgkdt knetspsgqc gpeqlqcndg gcylplqrcd
     6781 fipdckdatd erdcgtscdf ekdfcgwyss lsveerwyrt lgdrgdhtlg tskghylksg
     6841 vgvprpsgar aqlhskifre sgpecsfsfw fyspeeisma flnvyvkqak aspkidqvfa
     6901 laekemaqnr wepvkiflgr riqfgvivea vwgatgraam hiddflyqkc rpdhhassct
     6961 dsewmcadlt qciaefercd gkrdcsdgsd erdcvrgfgd csfdeedwaa acnwtvenva
     7021 gqpswkraka shsehtgpps shrgtpetyf llanstelpm gavsiartpq lpasqekchl
     7081 rfwyfmkgsp smeflrvete gagsrlpmwq elgpqgplwa yahvlvghpq pftvnflaqr
     7141 ggdaltdiai devtftpscl eggaadlkky sslcvegefl cadrkmcipr sfvcdcesdc
     7201 edgsdetdcg mkckgrgtta sgkatgrplt panetiqttp tpacafkefs cgghnghcip
     7261 alllcdgvpd cangdderqc dsrdrcpdgy yycrdphscl hqsklcdgra dcsdgsdesl
     7321 cnvcpdyfcl nggdcnlsqq trapscfcrd vfggnrcare ltveppspmg qtrvsgaagw
     7381 aygaplvalf vgaaavvavv ywrkrralsg eettpvtinn ptyglhleda svpsldiggd
     7441 tstsavnply sdc
//
LOCUS       XP_077503877             212 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114180 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503877
VERSION     XP_077503877.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647751.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..212
                     /product="uncharacterized protein LOC144114180 isoform X1"
                     /calculated_mol_wt=24469
     CDS             1..212
                     /gene="LOC144114180"
                     /coded_by="XM_077647751.1:1100..1738"
                     /db_xref="GeneID:144114180"
ORIGIN      
        1 mfiaaqfrss tytyvtmtsk clvtfrernf vlpfdgtltr rglvervrdh elfrgidvat
       61 liftmfeedf nvfvdipedf eirdrekisl rglpgsdcgc srrapasgai pchchvqphi
      121 qpasctkryp iqyrahqsgq yfkarsrvvq wlyhdlclyt mtlgeklsvs rqnmsgeksv
      181 ssrmmgqilh rqknqrkmgk wprlaempcs vf
//
LOCUS       XP_077503878             212 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114180 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503878
VERSION     XP_077503878.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647752.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..212
                     /product="uncharacterized protein LOC144114180 isoform X1"
                     /calculated_mol_wt=24469
     CDS             1..212
                     /gene="LOC144114180"
                     /coded_by="XM_077647752.1:1100..1738"
                     /db_xref="GeneID:144114180"
ORIGIN      
        1 mfiaaqfrss tytyvtmtsk clvtfrernf vlpfdgtltr rglvervrdh elfrgidvat
       61 liftmfeedf nvfvdipedf eirdrekisl rglpgsdcgc srrapasgai pchchvqphi
      121 qpasctkryp iqyrahqsgq yfkarsrvvq wlyhdlclyt mtlgeklsvs rqnmsgeksv
      181 ssrmmgqilh rqknqrkmgk wprlaempcs vf
//
LOCUS       XP_077503880             212 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114180 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503880
VERSION     XP_077503880.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647754.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..212
                     /product="uncharacterized protein LOC144114180 isoform X1"
                     /calculated_mol_wt=24469
     CDS             1..212
                     /gene="LOC144114180"
                     /coded_by="XM_077647754.1:1100..1738"
                     /db_xref="GeneID:144114180"
ORIGIN      
        1 mfiaaqfrss tytyvtmtsk clvtfrernf vlpfdgtltr rglvervrdh elfrgidvat
       61 liftmfeedf nvfvdipedf eirdrekisl rglpgsdcgc srrapasgai pchchvqphi
      121 qpasctkryp iqyrahqsgq yfkarsrvvq wlyhdlclyt mtlgeklsvs rqnmsgeksv
      181 ssrmmgqilh rqknqrkmgk wprlaempcs vf
//
LOCUS       XP_077503881             212 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114180 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503881
VERSION     XP_077503881.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647755.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..212
                     /product="uncharacterized protein LOC144114180 isoform X1"
                     /calculated_mol_wt=24469
     CDS             1..212
                     /gene="LOC144114180"
                     /coded_by="XM_077647755.1:1100..1738"
                     /db_xref="GeneID:144114180"
ORIGIN      
        1 mfiaaqfrss tytyvtmtsk clvtfrernf vlpfdgtltr rglvervrdh elfrgidvat
       61 liftmfeedf nvfvdipedf eirdrekisl rglpgsdcgc srrapasgai pchchvqphi
      121 qpasctkryp iqyrahqsgq yfkarsrvvq wlyhdlclyt mtlgeklsvs rqnmsgeksv
      181 ssrmmgqilh rqknqrkmgk wprlaempcs vf
//
LOCUS       XP_077503882             191 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114180 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503882
VERSION     XP_077503882.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647756.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..191
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..191
                     /product="uncharacterized protein LOC144114180 isoform X2"
                     /calculated_mol_wt=20961
     CDS             1..191
                     /gene="LOC144114180"
                     /coded_by="XM_077647756.1:1246..1821"
                     /db_xref="GeneID:144114180"
ORIGIN      
        1 mscsaastlp psssrcskki lmflltylkt skfgtgkksv sedcqvriad vldepqqaeq
       61 srvtatyslt yslpavpndi qfsieptkvd stskhaaels ngsimtsayt pctlasctmr
      121 lrklwcpgtq twlthlapam tlgeklsvsr qnmsgeksvs srmmgqilhr qknqrkmgkw
      181 prlaempcsv f
//
LOCUS       XP_077503883             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114180 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503883
VERSION     XP_077503883.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647757.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144114180 isoform X3"
                     /calculated_mol_wt=17221
     CDS             1..158
                     /gene="LOC144114180"
                     /coded_by="XM_077647757.1:1244..1720"
                     /db_xref="GeneID:144114180"
ORIGIN      
        1 mscsaastlp psssrcskki lmflltylkt skfgtgkksv sedcqvriad vldepqqaeq
       61 srvtatyslt yslpavpndi qfsieptkvd stskhaaels ngsimtsayt pctlasctmr
      121 lrklwcpgtq twlthlapam qlhqtgrmrr vllatske
//
LOCUS       XP_077503884             119 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114180 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503884
VERSION     XP_077503884.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647758.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..119
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..119
                     /product="uncharacterized protein LOC144114180 isoform X4"
                     /calculated_mol_wt=12689
     CDS             1..119
                     /gene="LOC144114180"
                     /coded_by="XM_077647758.1:1248..1607"
                     /db_xref="GeneID:144114180"
ORIGIN      
        1 mscsaastlp psssrcskki lmflltylkt skfgtgkksv sedcqvriad vldepqqaeq
       61 srvtatyslt yslpavpndi qfsieptkvd stskhaaels ngsimtsayt pcnctrrgg
//
LOCUS       XP_077503885             412 aa            linear   INV 25-NOV-2025
DEFINITION  thyrotropin-releasing hormone receptor-like [Amblyomma americanum].
ACCESSION   XP_077503885
VERSION     XP_077503885.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647759.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..412
                     /product="thyrotropin-releasing hormone receptor-like"
                     /calculated_mol_wt=45721
     Region          57..347
                     /region_name="7tmA_TRH-R"
                     /note="thyrotropin-releasing hormone receptor, member of
                     the class A family of seven-transmembrane G
                     protein-coupled receptors; cd14995"
                     /db_xref="CDD:320126"
     Region          58..84
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320126"
     Region          91..115
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320126"
     Site            order(113,116..117,131..136,138..139,142,187,189..193,222,
                     225..227,229..231,233..234,295,298..299,301..302,305,
                     314..315,317..319,322,325..326)
                     /site_type="other"
                     /note="putative peptide ligand binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:320126"
     Region          131..161
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320126"
     Region          174..195
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320126"
     Region          222..251
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320126"
     Region          254..305
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320126"
     Region          315..340
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320126"
     CDS             1..412
                     /gene="LOC144114181"
                     /coded_by="XM_077647759.1:236..1474"
                     /db_xref="GeneID:144114181"
ORIGIN      
        1 malaafllpa llptlvmtel ttnapawdga wnnttsappg pnrtyqdpty ysihyriigt
       61 ifqgvifaag vlgnimvviv vmrtrsmhtp tncylvslsv adfmvlvssv pneiisyyvl
      121 gdewiwgrag calfiflqyl ginasslsit aftveryiai clpmkaqtmc tvkrakkiil
      181 gvwlfactyc spwlaltttt avyykghdni etctfalsrh hyrsyffadi ilfyvvplll
      241 scvlyglmar vlfqsnfdsk angttdskks ssnsssrvqv vkmlfvvvav fatlwlpyrv
      301 llvynsfaek rymelwylmf cktmifinsa inpilynams ikfrrafkrm lscrgaqgsr
      361 dsrggtertv agtyssvvgg gsvrlnrsti vapagkgsts knalvdqshq lv
//
LOCUS       XP_077503886             165 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114182 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503886
VERSION     XP_077503886.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647760.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..165
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..165
                     /product="uncharacterized protein LOC144114182 isoform X1"
                     /calculated_mol_wt=17918
     CDS             1..165
                     /gene="LOC144114182"
                     /coded_by="XM_077647760.1:347..844"
                     /db_xref="GeneID:144114182"
ORIGIN      
        1 mscmkaagvl hledwtgkty vsaspsktah wlafepknyq kleiaavcas aamglvqsha
       61 acgghhilkt fsgrrppshp psptrsflpw iqlphlrsde srrlagtifa anlrktlpka
      121 arndsparta lsptakdwnr lpadavhhtn aahlkafiek tidml
//
LOCUS       XP_077503887             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114182 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503887
VERSION     XP_077503887.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647761.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144114182 isoform X2"
                     /calculated_mol_wt=17732
     CDS             1..158
                     /gene="LOC144114182"
                     /coded_by="XM_077647761.1:120..596"
                     /db_xref="GeneID:144114182"
ORIGIN      
        1 malqsapqts aqvlhserrr celfldqati lfhhrnqltr evaeafhisk nkltpisgps
       61 dvlherrrpp shppsptrsf lpwiqlphlr sdesrrlagt ifaanlrktl pkaarndspa
      121 rtalsptakd wnrlpadavh htnaahlkaf iektidml
//
LOCUS       XP_077503888             767 aa            linear   INV 25-NOV-2025
DEFINITION  protein turtle homolog A-like [Amblyomma americanum].
ACCESSION   XP_077503888
VERSION     XP_077503888.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647762.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..767
                     /product="protein turtle homolog A-like"
                     /calculated_mol_wt=82850
     Region          36..151
                     /region_name="V-set"
                     /note="Immunoglobulin V-set domain; pfam07686"
                     /db_xref="CDD:462230"
     Region          163..248
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          279..340
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          282..286
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          296..300
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          321..325
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          370..452
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          383..387
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          397..401
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          417..421
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          431..436
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          444..447
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          474..536
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cd00096"
                     /db_xref="CDD:409353"
     Region          474..478
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          488..492
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          512..516
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          526..531
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..767
                     /gene="LOC144114183"
                     /coded_by="XM_077647762.1:87..2390"
                     /db_xref="GeneID:144114183"
ORIGIN      
        1 masarrasga wartdvpaal laitlalasa aptepepqqv lalpgsrarl pcdlggsfga
       61 ssveaapsli twtrgpaate plysvgrspg valvqadhrm apawkdrlhf svsrgtlsla
      121 dvspgdagly vcrvrfqhap drmsrvqlvv vespgnpvlk fdpeaprgir lregqrlrvl
      181 cevvggkprp slrwslslsg gsqptpleat egssdardgv trswatldkl qrwhhearlt
      241 cqafnselva spssslrlql elkpaqvrik gsrspvsadv eaviecettg sqppanvswr
      301 leeaslrqtq llaqgangta sllrwtprpq dngrrlvcla lndhfpedgl edvwpldvhy
      361 kpivelrlgq gldssgvqeg hdayfecsvr anpavsevaw sldgrrlsga rlivsnlsla
      421 lrqvtrhqag lyrctaqnsa gqtvsnavrl rvkhapvcqr eesalflsav dedvelpcam
      481 laeppqlqfa wslngslgrr ditgfqshga rsslkfrpks evdygtlect arnsvgpair
      541 pcifhllrpp gntwlsrkwy ncsvwnatss avqvvcqrgl vpsaadpgal mdgdsgdeep
      601 qltlelrrpl sgttggtnas rassaawsrt qagrrslwlr glapdsplel lvfadsprgr
      661 sqlaalraqt lpeassvpgs graqerrtqs hpvvpdatqk eeeppeegga rqehagccpp
      721 lvtlacalsa avlvllpaav liglrlrtry yrrrrrppei yslqevk
//
LOCUS       XP_077503889             682 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier family 23 member 1-like [Amblyomma americanum].
ACCESSION   XP_077503889
VERSION     XP_077503889.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647763.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..682
                     /product="solute carrier family 23 member 1-like"
                     /calculated_mol_wt=73164
     Region          111..604
                     /region_name="UraA"
                     /note="Xanthine/uracil permease [Nucleotide transport and
                     metabolism]; COG2233"
                     /db_xref="CDD:441834"
     CDS             1..682
                     /gene="LOC144114186"
                     /coded_by="XM_077647763.1:302..2350"
                     /db_xref="GeneID:144114186"
ORIGIN      
        1 mdpesesqgg vkedasheha grprvgshah engqpvtape eavedsaels ekeplfarke
       61 vlngedvfdf sktptetlep rngetrltfe keaddkpska qkvppermms ygvddspawy
      121 fsavlglqhy liattgtlsy pfalapalcm reedpargyl istiffisgi gtliqtslgi
      181 rlpiiqgcsv tflvpilatm alphwrcpte eeliaardpd sgltgnvtdd ewtavwqarm
      241 seisgaiias safevllgft givgfllrwv tplgiapcia lvglslfkea aqlssgnwga
      301 afmsiilmti fsqyfqnvti pvpgwergkg cvvkhfaifk lfpillaili swgacflmtn
      361 tdylppgdpa rtdirasiid kspwirvpyp gqfgmprvsl gvvlgmlsai lssvvesvgd
      421 yyacarlsqv ptppthainr giwmeglgci lagiwgggcg ltsystnisi iavtkvacrs
      481 vvqwaavfmi gfgvvgkiga lfatipdpii ggvfcimfam iaavglssaq cvdlnssrnl
      541 fvlgtslffg lmvshwtshh pdaiktgydv ldqtilills tsmfvggflg ifldntipgt
      601 peerglgsgg hhggpggsgs gdegeftkdc ydlpflrarl anaafsylpi sptysnaall
      661 qritpkfvrs lsrrsnkgas ea
//
LOCUS       XP_077503890             260 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114187 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503890
VERSION     XP_077503890.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647764.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..260
                     /product="uncharacterized protein LOC144114187 isoform X1"
                     /calculated_mol_wt=28990
     Region          17..66
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(18,21,36,41,44,47,60,63)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     CDS             1..260
                     /gene="LOC144114187"
                     /coded_by="XM_077647764.1:10..792"
                     /db_xref="GeneID:144114187"
ORIGIN      
        1 mairrhalsi pqtgpvfcrl clkgreidal mvaclctgdn tfvhkscier svtqsgvtsc
       61 efcgyrlrir rvskpllqwl vsvesredvv llllnlvast gdvlaftlaw lraltyleqe
      121 qgapraahlf vsgflscltc fwswfqgyrl csmkekessm kessidainf tqqllqlddq
      181 katgpdgipt gflkryftdp irrwrrdtst lcvlsvtvgr rgalerhske teigslgqla
      241 cdshgdeqcl tgsqsrqvls
//
LOCUS       XP_077503891             216 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114187 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503891
VERSION     XP_077503891.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647765.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..216
                     /product="uncharacterized protein LOC144114187 isoform X2"
                     /calculated_mol_wt=24206
     Region          17..66
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(18,21,36,41,44,47,60,63)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     CDS             1..216
                     /gene="LOC144114187"
                     /coded_by="XM_077647765.1:10..660"
                     /db_xref="GeneID:144114187"
ORIGIN      
        1 mairrhalsi pqtgpvfcrl clkgreidal mvaclctgdn tfvhkscier svtqsgvtsc
       61 efcgyrlrir rvskpllqwl vsvesredvv llllnlvast gdvlaftlaw lraltyleqe
      121 qgapraahlf vsgflscltc fwswfqgyrl wyftdpirrw rrdtstlcvl svtvgrrgal
      181 erhsketeig slgqlacdsh gdeqcltgsq srqvls
//
LOCUS       XP_077503893             443 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein CG3556 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503893
VERSION     XP_077503893.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647767.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="uncharacterized protein CG3556 isoform X1"
                     /calculated_mol_wt=48220
     Region          100..330
                     /region_name="Cobalamin_bind"
                     /note="Eukaryotic cobalamin-binding protein; pfam01122"
                     /db_xref="CDD:460073"
     CDS             1..443
                     /gene="LOC144114189"
                     /coded_by="XM_077647767.1:158..1489"
                     /db_xref="GeneID:144114189"
ORIGIN      
        1 mkgsrddrga rrwvrslaav allsivplav ngfqldgppd pqgalateee algrarswlk
       61 ahpchqykdt aapgeahqrt lalalvdpaf fdrcsaeall hvrelqhqll vqlpttaekg
      121 qlalhvlals ascqpirdpr grnlgvllrk kssdpvprnr fseamqllal cslgdrgrkl
      181 mdeamanvqe lathnpaavm dslavnvlaa aclsdnelge rsttvydalr pvarlqqpdg
      241 sfgnvhttgl vtqalvavdp egqhlawrrq daltnlrraq hsdghfggll atvqvaplla
      301 grtladlash arvfctsrnn vsaatphepe eitvwyslwm gddatnrsst tvwvrenatv
      361 fdvmnagaqk drrfrfeydt asglgpyvvs lsgvprnvea ghfwlahvmn fdtglvtsls
      421 vgigthvvkd sdhvimwykh vvy
//
LOCUS       XP_077503894             406 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein CG3556 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503894
VERSION     XP_077503894.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647768.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..406
                     /product="uncharacterized protein CG3556 isoform X2"
                     /calculated_mol_wt=44230
     Region          63..293
                     /region_name="Cobalamin_bind"
                     /note="Eukaryotic cobalamin-binding protein; pfam01122"
                     /db_xref="CDD:460073"
     CDS             1..406
                     /gene="LOC144114189"
                     /coded_by="XM_077647768.1:160..1380"
                     /db_xref="GeneID:144114189"
ORIGIN      
        1 mafssgalat eeealgrars wlkahpchqy kdtaapgeah qrtlalalvd paffdrcsae
       61 allhvrelqh qllvqlptta ekgqlalhvl alsascqpir dprgrnlgvl lrkkssdpvp
      121 rnrfseamql lalcslgdrg rklmdeaman vqelathnpa avmdslavnv laaaclsdne
      181 lgersttvyd alrpvarlqq pdgsfgnvht tglvtqalva vdpegqhlaw rrqdaltnlr
      241 raqhsdghfg gllatvqvap llagrtladl asharvfcts rnnvsaatph epeeitvwys
      301 lwmgddatnr ssttvwvren atvfdvmnag aqkdrrfrfe ydtasglgpy vvslsgvprn
      361 veaghfwlah vmnfdtglvt slsvgigthv vkdsdhvimw ykhvvy
//
LOCUS       XP_077503895             344 aa            linear   INV 25-NOV-2025
DEFINITION  cellular tumor antigen p53-like [Amblyomma americanum].
ACCESSION   XP_077503895
VERSION     XP_077503895.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647769.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..344
                     /product="cellular tumor antigen p53-like"
                     /calculated_mol_wt=37355
     Region          79..253
                     /region_name="P53"
                     /note="P53 DNA-binding domain; pfam00870"
                     /db_xref="CDD:459972"
     CDS             1..344
                     /gene="LOC144114190"
                     /coded_by="XM_077647769.1:475..1509"
                     /db_xref="GeneID:144114190"
ORIGIN      
        1 mthagllelw esecggqdge thgptppssv wnehnnallw panadealhp gdamflellg
       61 pdvtqsyrla pntssvnqsv aewpgdfgfs aviagparag knvtwtysee lrklyvtmns
      121 pcpirciter appdgsfvcv vavfkesdsr qenvlrcpah cmaadalrig alkgqwfdcd
      181 hpeaqyccdn anggrhcirl plgsdvfhlt fgcrnscagg psrrateivf llqspegqdl
      241 arsvvqvkvc acpgrdrrrd eegreherav pptqaafpst smlrdvgtdg sssdsggesr
      301 ggtltlrvkd revhrvlthf lenaqalsvp dlrtrrdpss esdd
//
LOCUS       XP_077503896             310 aa            linear   INV 25-NOV-2025
DEFINITION  aaRS-interacting multifunctional protein 1 [Amblyomma americanum].
ACCESSION   XP_077503896
VERSION     XP_077503896.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647770.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..310
                     /product="aaRS-interacting multifunctional protein 1"
                     /calculated_mol_wt=33605
     Region          <29..67
                     /region_name="bZIP"
                     /note="Basic leucine zipper (bZIP) domain of bZIP
                     transcription factors: a DNA-binding and dimerization
                     domain; cl21462"
                     /db_xref="CDD:473870"
     Region          <98..310
                     /region_name="PLN02610"
                     /note="probable methionyl-tRNA synthetase"
                     /db_xref="CDD:215329"
     CDS             1..310
                     /gene="AIMP1"
                     /coded_by="XM_077647770.1:127..1059"
                     /db_xref="GeneID:144114191"
ORIGIN      
        1 mvvgnalarv msrakqadel iamltaqlqs lraarteqyv qaecarlrse nevlrrevea
       61 wknrleaaes rngavpvals tqpttsrqpa hptdsrpaar vpsnpgdtga gdqlkkragp
      121 tadakeppak kagakkkved gddskpvdvs rldlrvgriv savkhpdads lyveqvdvge
      181 ekhrtvvsgl vkfvplekmq nrmavfmcnl kpakmrgvts eamvmcastp ekvdilappp
      241 nalpgdrvic dgypgqpdaq lnpkkkifeq vapdlktdne kratykgqpw lvagrqgyit
      301 aetlcnaqik
//
LOCUS       XP_077503897             646 aa            linear   INV 25-NOV-2025
DEFINITION  lysyl-tRNA synthetase isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503897
VERSION     XP_077503897.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647771.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..646
                     /product="lysyl-tRNA synthetase isoform X1"
                     /calculated_mol_wt=72654
     Region          71..628
                     /region_name="PLN02502"
                     /note="lysyl-tRNA synthetase"
                     /db_xref="CDD:215278"
     Site            320..324
                     /site_type="other"
                     /note="motif 1"
                     /db_xref="CDD:238398"
     Site            order(352,374,376,382,386,538,545,548,552,604)
                     /site_type="active"
                     /db_xref="CDD:238398"
     Site            373..376
                     /site_type="other"
                     /note="motif 2"
                     /db_xref="CDD:238398"
     Site            601..604
                     /site_type="other"
                     /note="motif 3"
                     /db_xref="CDD:238398"
     CDS             1..646
                     /gene="LysRS"
                     /coded_by="XM_077647771.1:31..1971"
                     /db_xref="GeneID:144114192"
ORIGIN      
        1 mclryaflci dmvighqpkn grrrrkveqk vtmmalrtlc qtlrrhsslv lpvvpsastc
       61 hickprtlva saraseqkrr qkaeqkakek aeraealkaa aaaaaptsnn aapadakdee
      121 tmdpneffri rsamvaqlre agedpyphkf dvsislvefv erysdlqagq vltderlsva
      181 grvhakrqsg aklifydlrg egtkiqvman asyysseeaf akvnaklrrg dilgcighpg
      241 ktkkgelsiv pvemvllapc lhmlphlhfg lkdketrfrq ryldlilnds trqkfitrsk
      301 vvnyvrkffd nmgflevetp mmnmiaggat akpfithhne lnmdlymrva pelylkmliv
      361 ggldrvyeig rqfrnegidl thnpefttce fymayadynd lmtiteslvs gmvkfltgsy
      421 kvtyhpegad gpaleidftp pfrrvnmlpd lekamgvklp apellgtpes vallsdlcik
      481 hevecpaprt aarlldklvg hfleeqfvnp tflcnhpelm splskwhrsv rglterfelf
      541 varkeivnay telndpliqr qrfeqqakdk aagddeaqlv denfctaley glpptggwgm
      601 gidrlvmflt dsynikamll fpamkpddph khrieegada vnapgn
//
LOCUS       XP_077503898             597 aa            linear   INV 25-NOV-2025
DEFINITION  lysyl-tRNA synthetase isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503898
VERSION     XP_077503898.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647772.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..597
                     /product="lysyl-tRNA synthetase isoform X2"
                     /calculated_mol_wt=66913
     Region          16..579
                     /region_name="PLN02502"
                     /note="lysyl-tRNA synthetase"
                     /db_xref="CDD:215278"
     Site            271..275
                     /site_type="other"
                     /note="motif 1"
                     /db_xref="CDD:238398"
     Site            order(303,325,327,333,337,489,496,499,503,555)
                     /site_type="active"
                     /db_xref="CDD:238398"
     Site            324..327
                     /site_type="other"
                     /note="motif 2"
                     /db_xref="CDD:238398"
     Site            552..555
                     /site_type="other"
                     /note="motif 3"
                     /db_xref="CDD:238398"
     CDS             1..597
                     /gene="LysRS"
                     /coded_by="XM_077647772.1:27..1820"
                     /db_xref="GeneID:144114192"
ORIGIN      
        1 mpscastwsl vispkmadag eklskneqkr rqkaeqkake kaeraealka aaaaaaptsn
       61 naapadakde etmdpneffr irsamvaqlr eagedpyphk fdvsislvef verysdlqag
      121 qvltderlsv agrvhakrqs gaklifydlr gegtkiqvma nasyysseea fakvnaklrr
      181 gdilgcighp gktkkgelsi vpvemvllap clhmlphlhf glkdketrfr qryldlilnd
      241 strqkfitrs kvvnyvrkff dnmgflevet pmmnmiagga takpfithhn elnmdlymrv
      301 apelylkmli vggldrvyei grqfrnegid lthnpefttc efymayadyn dlmtiteslv
      361 sgmvkfltgs ykvtyhpega dgpaleidft ppfrrvnmlp dlekamgvkl papellgtpe
      421 svallsdlci khevecpapr taarlldklv ghfleeqfvn ptflcnhpel msplskwhrs
      481 vrglterfel fvarkeivna ytelndpliq rqrfeqqakd kaagddeaql vdenfctale
      541 yglpptggwg mgidrlvmfl tdsynikaml lfpamkpddp hkhrieegad avnapgn
//
LOCUS       XP_077503899            1147 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114193 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503899
VERSION     XP_077503899.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647773.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1147
                     /product="uncharacterized protein LOC144114193 isoform X1"
                     /calculated_mol_wt=122346
     Region          7..85
                     /region_name="BRCT_2"
                     /note="BRCT domain, a BRCA1 C-terminus domain; pfam16589"
                     /db_xref="CDD:465186"
     Region          115..183
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cl21498"
                     /db_xref="CDD:473887"
     Region          <178..299
                     /region_name="DMP1"
                     /note="Dentin matrix protein 1 (DMP1); pfam07263"
                     /db_xref="CDD:462128"
     CDS             1..1147
                     /gene="LOC144114193"
                     /coded_by="XM_077647773.1:93..3536"
                     /db_xref="GeneID:144114193"
ORIGIN      
        1 mpkkqrsral fakadgspml fslvpcserk eirelieygg gvlvppekvp dairlvpssv
       61 tvtdssddvf sanyiracak rgkllrlief kipmapavvd asdsktrrlh strsrreytp
      121 geeiaiaefv aknpgvhvhg navykemasa gvlpgkhswq slkehylkni fpvkhlyesp
      181 hevnvlsgkq ssesagdrgs spllaaassv ivedsdseqe gapaevvaet esssplqssg
      241 tspdraridk esdngrelll faspsaksae kssesgldsp lakkndpdhs tstsdqgsta
      301 valrkhslpt psevlckkqk elsistasvc vrsrhslrka stsrvdvpdt pgqepilnee
      361 rglrsslegk cknstprkqr iprhmksgsq isgigrsart tvsesrntlq rtpvkfhtlt
      421 pvkpnsaalq dcssshlgkq plpassgssi lgdvvkctpc psrhvplris lrkrpntppq
      481 eltnshsnsq sspkssgdva vmpkvkrgtl spgrfhggds gameglfert pflsrqpvar
      541 ssprkrqspe pqkdcgshss twrccesadd tmpdpedllp psharrrllp cpspanshkk
      601 kviplrsehr pdsvssteyk ctdhshtsns sprkqrkire eghnnvshqn pspsssmpth
      661 emassataps trtssgiytr scsllqqpsl elglraptak ttqgrkkgpr appppqcged
      721 ghsssasqaa gtgknhsapl gpgaevedld sadedllmea llqetalrnl gpmpgvaevh
      781 pvgatkeahr ddvslqkpsl esglraptak targskkapq apslpqyged ghsssashga
      841 grgknqsapl gtgaevedld sadedllmea llqeaalrnl gpmpgvaevh pvgatkeahr
      901 ddvslqkpsl esglraptak targskkapq apslpqyged ghsssasqga gtgknqsapl
      961 gtgaevedld sadedllmea llqeaavrnl gpipgvaeih pvgatkeahr dnrsdagspa
     1021 stlsvpstvi isdsghspde dstpefeqde vskvrrrlvr llgmlregkn teshetcpah
     1081 cgcprttryh rtvrevisls aflayhgepl psespgiedp eiallieill rdregrdkne
     1141 qdgsgsa
//
LOCUS       XP_077503900            1037 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114193 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503900
VERSION     XP_077503900.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647774.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1037
                     /product="uncharacterized protein LOC144114193 isoform X2"
                     /calculated_mol_wt=111073
     Region          7..85
                     /region_name="BRCT_2"
                     /note="BRCT domain, a BRCA1 C-terminus domain; pfam16589"
                     /db_xref="CDD:465186"
     Region          115..183
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cl21498"
                     /db_xref="CDD:473887"
     Region          <178..299
                     /region_name="DMP1"
                     /note="Dentin matrix protein 1 (DMP1); pfam07263"
                     /db_xref="CDD:462128"
     CDS             1..1037
                     /gene="LOC144114193"
                     /coded_by="XM_077647774.1:93..3206"
                     /db_xref="GeneID:144114193"
ORIGIN      
        1 mpkkqrsral fakadgspml fslvpcserk eirelieygg gvlvppekvp dairlvpssv
       61 tvtdssddvf sanyiracak rgkllrlief kipmapavvd asdsktrrlh strsrreytp
      121 geeiaiaefv aknpgvhvhg navykemasa gvlpgkhswq slkehylkni fpvkhlyesp
      181 hevnvlsgkq ssesagdrgs spllaaassv ivedsdseqe gapaevvaet esssplqssg
      241 tspdraridk esdngrelll faspsaksae kssesgldsp lakkndpdhs tstsdqgsta
      301 valrkhslpt psevlckkqk elsistasvc vrsrhslrka stsrvdvpdt pgqepilnee
      361 rglrsslegk cknstprkqr iprhmksgsq isgigrsart tvsesrntlq rtpvkfhtlt
      421 pvkpnsaalq dcssshlgkq plpassgssi lgdvvkctpc psrhvplris lrkrpntppq
      481 eltnshsnsq sspkssgdva vmpkvkrgtl spgrfhggds gameglfert pflsrqpvar
      541 ssprkrqspe pqkdcgshss twrccesadd tmpdpedllp psharrrllp cpspanshkk
      601 kviplrsehr pdsvssteyk ctdhshtsns sprkqrkire eghnnvshqn pspsssmpth
      661 emassataps trtssgiytr scsllqqpsl elglraptak ttqgrkkgpr appppqcged
      721 ghsssasqaa gtgknhsapl gpgaevedld sadedllmea llqetalrnl gpmpgvaevh
      781 pvgatkeahr ddvslqkpsl esglraptak targskkapq apslpqyged ghsssasqga
      841 gtgknqsapl gtgaevedld sadedllmea llqeaavrnl gpipgvaeih pvgatkeahr
      901 dnrsdagspa stlsvpstvi isdsghspde dstpefeqde vskvrrrlvr llgmlregkn
      961 teshetcpah cgcprttryh rtvrevisls aflayhgepl psespgiedp eiallieill
     1021 rdregrdkne qdgsgsa
//
LOCUS       XP_077503901            1052 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114195 [Amblyomma americanum].
ACCESSION   XP_077503901
VERSION     XP_077503901.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647775.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1052
                     /product="uncharacterized protein LOC144114195"
                     /calculated_mol_wt=112831
     Region          7..85
                     /region_name="BRCT_2"
                     /note="BRCT domain, a BRCA1 C-terminus domain; pfam16589"
                     /db_xref="CDD:465186"
     Region          115..183
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cl21498"
                     /db_xref="CDD:473887"
     Region          <178..314
                     /region_name="DMP1"
                     /note="Dentin matrix protein 1 (DMP1); pfam07263"
                     /db_xref="CDD:462128"
     CDS             1..1052
                     /gene="LOC144114195"
                     /coded_by="XM_077647775.1:100..3258"
                     /db_xref="GeneID:144114195"
ORIGIN      
        1 mpkkqrsral fakadgspml fslvpcserk eirelieygg gvlvqpekvp dairlvpssv
       61 tvtdssddvf sanyiracak rdkllrlief kipmapavvd asdsktrrlh strsrreytl
      121 geeiaiaefv akkpgvhvhg navykemasa gvlpgkhswq slkehylkni fpvkhlyesp
      181 hevnvlsgkq ssesagdrgs spllaaassv ivedsdseqe arepqrpsgs glqdegapae
      241 vvaetesssp lqssgtspdr aridkesdng relllfasps aksaeksses gldsplakkn
      301 dpdhststsd qgstavalrk hslptpsevl ckkqkelsis tasvsvrsrh slreastsrv
      361 dvpdtpgqep ilneerglrs slegkcknst prkqpiprym kpgsqisgig rsarttvses
      421 rntlqrtpvk fhtltpvkpn saalqecsss hlgkqplpas sgssilgdvv kctpcpsrhi
      481 plrislrkrp ntppqeltns hsnsqsspks sgdvavmpkv krgtlspgrf hggdsgameg
      541 lfertpflsr qpvarssprk rqspepqkdc gshsstwrcc espddtmpdp edplppshar
      601 rrllpcpspa nshkkkvipl rsehrpdsvs steykctdhs htsnssprkq rkireeghnn
      661 vshqnpspss smpthemass atapstrtss giytrscsll qqpslelglr aptakttrgr
      721 kkgprapppp qcgedghsss asqaagtgkn hsaplgpgae vedldsaded llmeallqet
      781 alrnlgpipg vaevhpvgat keahrddvsl qkpslesglr apaaktargs kkapqapslp
      841 qygedghsss ashgagrgkn qsaplgtgae vedldsaded llmeallqea avrnlgpipg
      901 vaevhpvgat keahrdnrsd agspastlsv pstviisdsg hspdedstpe feqdevskvr
      961 rrlvrllgml regknteshe tcpahcgcpr ttryhrtvre vislsaflay hgeplpsesp
     1021 giedpeiall ieillrdreg rdkneqdgsg sa
//
LOCUS       XP_077503902            1162 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114196 [Amblyomma americanum].
ACCESSION   XP_077503902
VERSION     XP_077503902.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647776.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1162
                     /product="uncharacterized protein LOC144114196"
                     /calculated_mol_wt=123960
     Region          7..85
                     /region_name="BRCT_2"
                     /note="BRCT domain, a BRCA1 C-terminus domain; pfam16589"
                     /db_xref="CDD:465186"
     Region          115..183
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cl21498"
                     /db_xref="CDD:473887"
     Region          <178..314
                     /region_name="DMP1"
                     /note="Dentin matrix protein 1 (DMP1); pfam07263"
                     /db_xref="CDD:462128"
     CDS             1..1162
                     /gene="LOC144114196"
                     /coded_by="XM_077647776.1:95..3583"
                     /db_xref="GeneID:144114196"
ORIGIN      
        1 mpkkqrsral fakadgspml fslvpcserk eirelieygg gvlvppekvp dairlvpssv
       61 tvtdssddvf sanyiracak rgkllrlief kipmapavvd asdsktrrlh strsrreytp
      121 geeiaiaefv akkpgvhvhg navykemasa gvlpgkhswq slkehylkni fpvkhlyesp
      181 hevnvlsgkq ssesagdrgs spllaaassv ivedsdseqe arepqrpsgs glqdegapae
      241 vvaetesssp lqssgtspdr aridkesdng relllfasps aksaeksses gldsplakkn
      301 dpdhststsd qgstavalrk hslptpsevl ckkqkelsis tasvcvrsrh slrkastsrv
      361 dvpdtpgqep ilneerglrs slegkcknst prkqriprhm ksgsqisgig rsarttvses
      421 rntlqrtpvk fhtltpvkpn saalqdcsss hlgkqplpas sgssilgdvv kctpcpsrhv
      481 plrislrkrp ntppqeltns hsnsqsspks sgdvavmpkv krgtlspgrf hggdsgameg
      541 lfertpflsr qpvarssprk rqspepqkdc gshsstwrcc esaddtmpdp edllppshar
      601 rrllpcpspa nshkkkvipl rsehrpdsvs steykctdhs htsnssprkq rkireeghnn
      661 vshqnpspss smpthemass atapstrtss giytrscsll qqpslelglr aptakttqgr
      721 kkgprapppp qcgedghsss asqaagtgkn hsaplgpgae vedldsaded llmeallqet
      781 alrnlgpmpg vaevhpvgat keahrddvsl qkpslesglr aptaktargs kkapqapslp
      841 qygedghsss asqgagrgkn qsaplgtgae vedldsaded llmeallqea alrnlgpmpg
      901 vaevhpvgat keahrddvsl qkpslesglr aptaktargs kkapqapslp qygedghsss
      961 asqgagtgkn qsaplgtgae vedldsaded llmeallqea avrnlgpipg vaeihpvgat
     1021 keahrdnrsd agspastlsv pstviisdsg hspdedstpe feqdevskvr rrlvrllgml
     1081 regknteshe tcpahcgcpr ttryhrtvre vislsaflay hgeplpsesp giedpeiall
     1141 ieillrdreg rdkneqdgsg sa
//
LOCUS       XP_077503903            1052 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114197 [Amblyomma americanum].
ACCESSION   XP_077503903
VERSION     XP_077503903.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647777.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1052
                     /product="uncharacterized protein LOC144114197"
                     /calculated_mol_wt=112870
     Region          7..85
                     /region_name="BRCT_2"
                     /note="BRCT domain, a BRCA1 C-terminus domain; pfam16589"
                     /db_xref="CDD:465186"
     Region          115..183
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cl21498"
                     /db_xref="CDD:473887"
     Region          <178..314
                     /region_name="DMP1"
                     /note="Dentin matrix protein 1 (DMP1); pfam07263"
                     /db_xref="CDD:462128"
     CDS             1..1052
                     /gene="LOC144114197"
                     /coded_by="XM_077647777.1:100..3258"
                     /db_xref="GeneID:144114197"
ORIGIN      
        1 mpkkqrsral fakadgspml fslvpcserk eirelieygg gvlvqpekfp dairlvpssv
       61 tvtdssddvf sanyiracak rdkllrlief kipmapavvd asdsktrrlh strsrreytl
      121 geeiaiaefv akkpgvhvhg navykemasa gvlpgkhswq slkehylkni fpvkhlyesp
      181 hevnvlsgkq ssesagdrgs spllaaassv ivedsdseqe arepqrpsgs glqdegapae
      241 vvaetesssp lqssgtspdr aridkesdng relllfasps aksaeksses gldsplakkn
      301 dpdhststsd qgstavalrk hslptpsevl ckkqkelsis tasvsvrsrh slreastsrv
      361 dvpdtpgqep ilneerglrs slegkcknst prkqpiprym kpgsqisgig rsarttvses
      421 rntlqrtpvk fhtltpvkpn saalqecsss hlgkqplpas sgssilgdvv kctpcpsrhi
      481 plrislrkrp ntppqeltns hsnsqsspks sgdvavmpkv krgtlspgrf hggdsgameg
      541 lfertpflsr qpvarssprk rqspepqkdc gshsstwrcc espddtmpdp edplppshar
      601 rrllpcpspa nshkkkvipl rsehrpdsvs steykctdhs htsnssprkq rkireeghnn
      661 vshqnpspss smpthemass atapstrtss giytrscsll qqpslelglr aptakttrgr
      721 kkgprapppp qcgedghsss asqaagtgkn hsaplgpgae vedldsaded llmeallqet
      781 alrnlgpipg vaevhpvgat keahrddvsl qkpslesglr apaaktargs kkapqapslp
      841 qygedghsss asqgagrgkn qsaplgtgae vedldsaded llmeallqea avrnlgpipg
      901 vaevhpvgat keahrdnrsd agspastlsv pstviisdsg hspdedstpe feqdevskvr
      961 rrlvrllgml regknteshe tcpahcgcpr ttryhrtvre vislsaflay hgeplpsesp
     1021 giedpeiall ieillrdreg rdkneqdgsg sa
//
LOCUS       XP_077503904             271 aa            linear   INV 25-NOV-2025
DEFINITION  leukocyte receptor cluster member 1 homolog [Amblyomma americanum].
ACCESSION   XP_077503904
VERSION     XP_077503904.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647778.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..271
                     /product="leukocyte receptor cluster member 1 homolog"
                     /calculated_mol_wt=31533
     Region          8..44
                     /region_name="Cir_N"
                     /note="N-terminal domain of CBF1 interacting co-repressor
                     CIR; smart01083"
                     /db_xref="CDD:198151"
     CDS             1..271
                     /gene="LOC144114199"
                     /coded_by="XM_077647778.1:126..941"
                     /db_xref="GeneID:144114199"
ORIGIN      
        1 mnilpkkswh vrtkrnidrv rkdealaaqe ekelqrkiql aesearinfl rgklkaegqe
       61 efdfekaata evatdltsgg hfnffkdvek aernlhskne drekdkkaeq edyekkigll
      121 tylgqsssea sgskpwfmgs hedrlqlcee seketkdskt kdsmdplhli khyvtlkygv
      181 kentdkikkl nqiehqskrr lrkvkrlgks veelraerla rekaerlkae evlqrargep
      241 parekspdvi lddrrrgyns qynphlakrp q
//
LOCUS       XP_077503905             271 aa            linear   INV 25-NOV-2025
DEFINITION  leukocyte receptor cluster member 1 homolog [Amblyomma americanum].
ACCESSION   XP_077503905
VERSION     XP_077503905.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647779.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..271
                     /product="leukocyte receptor cluster member 1 homolog"
                     /calculated_mol_wt=31533
     Region          8..44
                     /region_name="Cir_N"
                     /note="N-terminal domain of CBF1 interacting co-repressor
                     CIR; smart01083"
                     /db_xref="CDD:198151"
     CDS             1..271
                     /gene="LOC144114199"
                     /coded_by="XM_077647779.1:247..1062"
                     /db_xref="GeneID:144114199"
ORIGIN      
        1 mnilpkkswh vrtkrnidrv rkdealaaqe ekelqrkiql aesearinfl rgklkaegqe
       61 efdfekaata evatdltsgg hfnffkdvek aernlhskne drekdkkaeq edyekkigll
      121 tylgqsssea sgskpwfmgs hedrlqlcee seketkdskt kdsmdplhli khyvtlkygv
      181 kentdkikkl nqiehqskrr lrkvkrlgks veelraerla rekaerlkae evlqrargep
      241 parekspdvi lddrrrgyns qynphlakrp q
//
LOCUS       XP_077503906             206 aa            linear   INV 25-NOV-2025
DEFINITION  mpv17-like protein 2 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503906
VERSION     XP_077503906.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647780.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..206
                     /product="mpv17-like protein 2 isoform X1"
                     /calculated_mol_wt=23739
     Region          131..190
                     /region_name="Mpv17_PMP22"
                     /note="Mpv17 / PMP22 family; pfam04117"
                     /db_xref="CDD:461182"
     CDS             1..206
                     /gene="LOC144114201"
                     /coded_by="XM_077647780.1:101..721"
                     /db_xref="GeneID:144114201"
ORIGIN      
        1 mrrcrtssel tgelhpmeyg alqlfrrfgr alfgrhlvat natistvmgi tgdlvqqhye
       61 ilcghqadin avrtshmaaa glttgmvchy wyvlldrwml grslrtvllk vlydqvvfsp
      121 iclavyfgtv sllerstwae lgrelwckgg tiyrvewvvw ppaqflnfyv lplryrvffd
      181 nlisfgfdvy spyikyrdqr thvshs
//
LOCUS       XP_077503907             190 aa            linear   INV 25-NOV-2025
DEFINITION  mpv17-like protein 2 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503907
VERSION     XP_077503907.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647781.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..190
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..190
                     /product="mpv17-like protein 2 isoform X2"
                     /calculated_mol_wt=21884
     Region          115..174
                     /region_name="Mpv17_PMP22"
                     /note="Mpv17 / PMP22 family; pfam04117"
                     /db_xref="CDD:461182"
     CDS             1..190
                     /gene="LOC144114201"
                     /coded_by="XM_077647781.1:139..711"
                     /db_xref="GeneID:144114201"
ORIGIN      
        1 meygalqlfr rfgralfgrh lvatnatist vmgitgdlvq qhyeilcghq adinavrtsh
       61 maaaglttgm vchywyvlld rwmlgrslrt vllkvlydqv vfspiclavy fgtvsllers
      121 twaelgrelw ckggtiyrve wvvwppaqfl nfyvlplryr vffdnlisfg fdvyspyiky
      181 rdqrthvshs
//
LOCUS       XP_077503908             190 aa            linear   INV 25-NOV-2025
DEFINITION  mpv17-like protein 2 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503908
VERSION     XP_077503908.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647782.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..190
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..190
                     /product="mpv17-like protein 2 isoform X2"
                     /calculated_mol_wt=21884
     Region          115..174
                     /region_name="Mpv17_PMP22"
                     /note="Mpv17 / PMP22 family; pfam04117"
                     /db_xref="CDD:461182"
     CDS             1..190
                     /gene="LOC144114201"
                     /coded_by="XM_077647782.1:306..878"
                     /db_xref="GeneID:144114201"
ORIGIN      
        1 meygalqlfr rfgralfgrh lvatnatist vmgitgdlvq qhyeilcghq adinavrtsh
       61 maaaglttgm vchywyvlld rwmlgrslrt vllkvlydqv vfspiclavy fgtvsllers
      121 twaelgrelw ckggtiyrve wvvwppaqfl nfyvlplryr vffdnlisfg fdvyspyiky
      181 rdqrthvshs
//
LOCUS       XP_077503909             190 aa            linear   INV 25-NOV-2025
DEFINITION  mpv17-like protein 2 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503909
VERSION     XP_077503909.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647783.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..190
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..190
                     /product="mpv17-like protein 2 isoform X2"
                     /calculated_mol_wt=21884
     Region          115..174
                     /region_name="Mpv17_PMP22"
                     /note="Mpv17 / PMP22 family; pfam04117"
                     /db_xref="CDD:461182"
     CDS             1..190
                     /gene="LOC144114201"
                     /coded_by="XM_077647783.1:188..760"
                     /db_xref="GeneID:144114201"
ORIGIN      
        1 meygalqlfr rfgralfgrh lvatnatist vmgitgdlvq qhyeilcghq adinavrtsh
       61 maaaglttgm vchywyvlld rwmlgrslrt vllkvlydqv vfspiclavy fgtvsllers
      121 twaelgrelw ckggtiyrve wvvwppaqfl nfyvlplryr vffdnlisfg fdvyspyiky
      181 rdqrthvshs
//
LOCUS       XP_077503910             190 aa            linear   INV 25-NOV-2025
DEFINITION  mpv17-like protein 2 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503910
VERSION     XP_077503910.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647784.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..190
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..190
                     /product="mpv17-like protein 2 isoform X2"
                     /calculated_mol_wt=21884
     Region          115..174
                     /region_name="Mpv17_PMP22"
                     /note="Mpv17 / PMP22 family; pfam04117"
                     /db_xref="CDD:461182"
     CDS             1..190
                     /gene="LOC144114201"
                     /coded_by="XM_077647784.1:204..776"
                     /db_xref="GeneID:144114201"
ORIGIN      
        1 meygalqlfr rfgralfgrh lvatnatist vmgitgdlvq qhyeilcghq adinavrtsh
       61 maaaglttgm vchywyvlld rwmlgrslrt vllkvlydqv vfspiclavy fgtvsllers
      121 twaelgrelw ckggtiyrve wvvwppaqfl nfyvlplryr vffdnlisfg fdvyspyiky
      181 rdqrthvshs
//
LOCUS       XP_077503911             120 aa            linear   INV 25-NOV-2025
DEFINITION  small ribonucleoprotein particle protein SmD1 [Amblyomma
            americanum].
ACCESSION   XP_077503911
VERSION     XP_077503911.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647785.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..120
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..120
                     /product="small ribonucleoprotein particle protein SmD1"
                     /calculated_mol_wt=13189
     Region          2..85
                     /region_name="Sm_D1"
                     /note="Sm protein D1; cd01724"
                     /db_xref="CDD:212471"
     Site            order(2,4,35..37,61..63)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:212471"
     Site            order(3,6..7,18..21,30..31,33..36,39,55,57..71,74,79)
                     /site_type="other"
                     /note="heptamer interface [polypeptide binding]"
                     /db_xref="CDD:212471"
     Site            order(15..21,23..35,37..41)
                     /site_type="other"
                     /note="Sm1 motif"
                     /db_xref="CDD:212471"
     Site            57..68
                     /site_type="other"
                     /note="Sm2 motif"
                     /db_xref="CDD:212471"
     CDS             1..120
                     /gene="SmD1"
                     /coded_by="XM_077647785.1:131..493"
                     /db_xref="GeneID:144114202"
ORIGIN      
        1 mklvrflmkl shetvtielk ngtqvhgtit gvdvamnthl kavkmtmrnr epvqldslsi
       61 rgnniryyll pdslpletll iddtpkakak kkeaaamgrg rgrgrgrgrg rggargrgrr
//
LOCUS       XP_077503912            1736 aa            linear   INV 25-NOV-2025
DEFINITION  jumonji, AT rich interactive domain 2 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503912
VERSION     XP_077503912.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647786.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1736
                     /product="jumonji, AT rich interactive domain 2 isoform
                     X1"
                     /calculated_mol_wt=190469
     Region          836..>1130
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     Region          1105..1145
                     /region_name="JmjN"
                     /note="Small domain found in the jumonji family of
                     transcription factors; smart00545"
                     /db_xref="CDD:128818"
     Region          1168..1278
                     /region_name="ARID_JARD2"
                     /note="ARID/BRIGHT DNA binding domain of Jumonji/ARID
                     domain-containing protein 2 (JARID2) and similar proteins;
                     cd16870"
                     /db_xref="CDD:350634"
     Site            order(1192..1198,1223,1225..1226,1229,1240,1242..1244,
                     1246)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:350634"
     Region          1434..1549
                     /region_name="JmjC"
                     /note="JmjC domain, hydroxylase; pfam02373"
                     /db_xref="CDD:396791"
     Region          1651..1703
                     /region_name="zf-C5HC2"
                     /note="C5HC2 zinc finger; pfam02928"
                     /db_xref="CDD:460750"
     CDS             1..1736
                     /gene="Jarid2"
                     /coded_by="XM_077647786.1:445..5655"
                     /db_xref="GeneID:144114203"
ORIGIN      
        1 mksafkerql sdysatsqrp krnastkglg csnglhwmee kqlkkalyas lnetkkarll
       61 denasedgre qapktttstv vakkaadtds krikvhaqrk faqgsnpssp mptpvkllpp
      121 cresrdtvsq pstllpckrr pktedfltfl clrgtaalpp aldfmsgtaf pgsssssdrs
      181 sspemdasla sttaeyrdrg asagalwgtv padvrttaqr aatrvpkqdr rpfrpesgee
      241 kqaseqrssr satakkmdkr kivpekskdt lkpgsiskcl skpngnltal qekykqrria
      301 lqtgpsrkqs alghdgghal rsravrggvh sgskravkss cvksreqlgs alpnrhsvsg
      361 rdeemaaknr lvlkrqlsvq ltrlhpriaa glrlpimass gtgvvtrrrs vgitphlrka
      421 kmvppivdye sesekdlspl spprspliav qprsratgsk thnasqkpks kvtvstttkk
      481 kptsviarkl lnrattrmrp tsavrkrtrc tmfrrktrsl gpvkeyplvl alptkrgagr
      541 fggtvakkka eglaakstkl kvpltrssel pqvsskassa aaqqklkkvt vfsqndgtlk
      601 rstrrvsseg tsdmrslrqr sdatatkckr ethkaiigas ektqkaiatk rrkvevsasn
      661 asshlfsdsd deplikvagr lsekkvlsat ekdchskqhk sgsaqksgvv tppvtgmlsr
      721 kkaasvkdkn klpeidnsrf fknslvsesf sepspsslse tiadaagdpl dtrgdfddle
      781 smsggveald alageisqvm scrlsfgsaq eiqslmtsef sgafdenvtg amllgtrdgr
      841 sasfegglnl lkrddtpski dastntsdeg mtllatstpe elvatpeevs vgtqtavcdt
      901 dapssvagle avsrtarrls ftgsetsave eqlsletqks lpaspkskss alpvqksasp
      961 snsttkpdtr agsaglglss esttrlsskq ahsstsvhga rltagkgape dkvappslss
     1021 aakrsaaldk grilqrhgsl nptlgsrasv trapkfspar vapvpkpgps krsktasdep
     1081 rrakltplds kkfpsendpa clvaaptyrp tademrdpis yiskirpeae kfgicriilp
     1141 psfqpeckvf ddmrftaynq yihkmlsrwg pnvqhtacik rcftkqgitd dqppliggie
     1201 ldlsylyhtv qnfggiqqvm ekkkwhrvad amhipkaaqd rvskvddayc kfvlpydlls
     1261 adekkaiele vladrerqma adsepsstsa dedeeeefsn ecvakvsrhf aslgshgrmk
     1321 qppawtdqsn trkgrsmsls vfsrvarntm smwykhepst eevekdywnl vidqrrhvcv
     1381 hagnidssvh gsgfptnrlv pfakhpwnlk vltnnpgtvl rcmgpvsgvt iptlhlsmly
     1441 ttgcwyrdph clpwveylht gankiwysvp ahssdrfrsa mkeimpqvck dsaiwlpsdc
     1501 amvpppqlve hgcplsrlvq ekgqfvvifp gaftstiacg ysvsesvyfa tqdwllqase
     1561 cfehmkasce pptfsfekll lgiaselren letcrwtlpl lqemcekeqr yrtqlvemgl
     1621 ktferlsted tadpkkskrp rlrpedsnhe cdfcrinchv smvvnlqddc lyclehavhc
     1681 lqrknmkswk llyaydmdel rsilrkleeh iesaeasqmk tdsepslskk kvpkkk
//
LOCUS       XP_077503913            1708 aa            linear   INV 25-NOV-2025
DEFINITION  jumonji, AT rich interactive domain 2 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503913
VERSION     XP_077503913.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647787.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1708
                     /product="jumonji, AT rich interactive domain 2 isoform
                     X2"
                     /calculated_mol_wt=187306
     Region          836..>1130
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     Region          1105..1145
                     /region_name="JmjN"
                     /note="Small domain found in the jumonji family of
                     transcription factors; smart00545"
                     /db_xref="CDD:128818"
     Region          1168..1278
                     /region_name="ARID_JARD2"
                     /note="ARID/BRIGHT DNA binding domain of Jumonji/ARID
                     domain-containing protein 2 (JARID2) and similar proteins;
                     cd16870"
                     /db_xref="CDD:350634"
     Site            order(1192..1198,1223,1225..1226,1229,1240,1242..1244,
                     1246)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:350634"
     Region          1406..1521
                     /region_name="JmjC"
                     /note="JmjC domain, hydroxylase; pfam02373"
                     /db_xref="CDD:396791"
     Region          1623..1675
                     /region_name="zf-C5HC2"
                     /note="C5HC2 zinc finger; pfam02928"
                     /db_xref="CDD:460750"
     CDS             1..1708
                     /gene="Jarid2"
                     /coded_by="XM_077647787.1:445..5571"
                     /db_xref="GeneID:144114203"
ORIGIN      
        1 mksafkerql sdysatsqrp krnastkglg csnglhwmee kqlkkalyas lnetkkarll
       61 denasedgre qapktttstv vakkaadtds krikvhaqrk faqgsnpssp mptpvkllpp
      121 cresrdtvsq pstllpckrr pktedfltfl clrgtaalpp aldfmsgtaf pgsssssdrs
      181 sspemdasla sttaeyrdrg asagalwgtv padvrttaqr aatrvpkqdr rpfrpesgee
      241 kqaseqrssr satakkmdkr kivpekskdt lkpgsiskcl skpngnltal qekykqrria
      301 lqtgpsrkqs alghdgghal rsravrggvh sgskravkss cvksreqlgs alpnrhsvsg
      361 rdeemaaknr lvlkrqlsvq ltrlhpriaa glrlpimass gtgvvtrrrs vgitphlrka
      421 kmvppivdye sesekdlspl spprspliav qprsratgsk thnasqkpks kvtvstttkk
      481 kptsviarkl lnrattrmrp tsavrkrtrc tmfrrktrsl gpvkeyplvl alptkrgagr
      541 fggtvakkka eglaakstkl kvpltrssel pqvsskassa aaqqklkkvt vfsqndgtlk
      601 rstrrvsseg tsdmrslrqr sdatatkckr ethkaiigas ektqkaiatk rrkvevsasn
      661 asshlfsdsd deplikvagr lsekkvlsat ekdchskqhk sgsaqksgvv tppvtgmlsr
      721 kkaasvkdkn klpeidnsrf fknslvsesf sepspsslse tiadaagdpl dtrgdfddle
      781 smsggveald alageisqvm scrlsfgsaq eiqslmtsef sgafdenvtg amllgtrdgr
      841 sasfegglnl lkrddtpski dastntsdeg mtllatstpe elvatpeevs vgtqtavcdt
      901 dapssvagle avsrtarrls ftgsetsave eqlsletqks lpaspkskss alpvqksasp
      961 snsttkpdtr agsaglglss esttrlsskq ahsstsvhga rltagkgape dkvappslss
     1021 aakrsaaldk grilqrhgsl nptlgsrasv trapkfspar vapvpkpgps krsktasdep
     1081 rrakltplds kkfpsendpa clvaaptyrp tademrdpis yiskirpeae kfgicriilp
     1141 psfqpeckvf ddmrftaynq yihkmlsrwg pnvqhtacik rcftkqgitd dqppliggie
     1201 ldlsylyhtv qnfggiqqvm ekkkwhrvad amhipkaaqd rvskvddayc kfvlpydlls
     1261 adekkaiele vladrerqma adsepsstsa dedeeeefsn ecvakgrsms lsvfsrvarn
     1321 tmsmwykhep steevekdyw nlvidqrrhv cvhagnidss vhgsgfptnr lvpfakhpwn
     1381 lkvltnnpgt vlrcmgpvsg vtiptlhlsm lyttgcwyrd phclpwveyl htgankiwys
     1441 vpahssdrfr samkeimpqv ckdsaiwlps dcamvpppql vehgcplsrl vqekgqfvvi
     1501 fpgaftstia cgysvsesvy fatqdwllqa secfehmkas cepptfsfek lllgiaselr
     1561 enletcrwtl pllqemceke qryrtqlvem glktferlst edtadpkksk rprlrpedsn
     1621 hecdfcrinc hvsmvvnlqd dclyclehav hclqrknmks wkllyaydmd elrsilrkle
     1681 ehiesaeasq mktdsepsls kkkvpkkk
//
LOCUS       XP_077503914            1515 aa            linear   INV 25-NOV-2025
DEFINITION  jumonji, AT rich interactive domain 2 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503914
VERSION     XP_077503914.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647788.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1515
                     /product="jumonji, AT rich interactive domain 2 isoform
                     X3"
                     /calculated_mol_wt=165061
     Region          836..>1035
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     Region          <1007..1113
                     /region_name="PRK14954"
                     /note="DNA polymerase III subunits gamma and tau;
                     Provisional"
                     /db_xref="CDD:184918"
     Region          1105..1145
                     /region_name="JmjN"
                     /note="Small domain found in the jumonji family of
                     transcription factors; smart00545"
                     /db_xref="CDD:128818"
     Region          1168..1278
                     /region_name="ARID_JARD2"
                     /note="ARID/BRIGHT DNA binding domain of Jumonji/ARID
                     domain-containing protein 2 (JARID2) and similar proteins;
                     cd16870"
                     /db_xref="CDD:350634"
     Site            order(1192..1198,1223,1225..1226,1229,1240,1242..1244,
                     1246)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:350634"
     Region          1434..>1496
                     /region_name="cupin_RmlC-like"
                     /note="RmlC-like cupin superfamily; cl40423"
                     /db_xref="CDD:477354"
     CDS             1..1515
                     /gene="Jarid2"
                     /coded_by="XM_077647788.1:447..4994"
                     /db_xref="GeneID:144114203"
ORIGIN      
        1 mksafkerql sdysatsqrp krnastkglg csnglhwmee kqlkkalyas lnetkkarll
       61 denasedgre qapktttstv vakkaadtds krikvhaqrk faqgsnpssp mptpvkllpp
      121 cresrdtvsq pstllpckrr pktedfltfl clrgtaalpp aldfmsgtaf pgsssssdrs
      181 sspemdasla sttaeyrdrg asagalwgtv padvrttaqr aatrvpkqdr rpfrpesgee
      241 kqaseqrssr satakkmdkr kivpekskdt lkpgsiskcl skpngnltal qekykqrria
      301 lqtgpsrkqs alghdgghal rsravrggvh sgskravkss cvksreqlgs alpnrhsvsg
      361 rdeemaaknr lvlkrqlsvq ltrlhpriaa glrlpimass gtgvvtrrrs vgitphlrka
      421 kmvppivdye sesekdlspl spprspliav qprsratgsk thnasqkpks kvtvstttkk
      481 kptsviarkl lnrattrmrp tsavrkrtrc tmfrrktrsl gpvkeyplvl alptkrgagr
      541 fggtvakkka eglaakstkl kvpltrssel pqvsskassa aaqqklkkvt vfsqndgtlk
      601 rstrrvsseg tsdmrslrqr sdatatkckr ethkaiigas ektqkaiatk rrkvevsasn
      661 asshlfsdsd deplikvagr lsekkvlsat ekdchskqhk sgsaqksgvv tppvtgmlsr
      721 kkaasvkdkn klpeidnsrf fknslvsesf sepspsslse tiadaagdpl dtrgdfddle
      781 smsggveald alageisqvm scrlsfgsaq eiqslmtsef sgafdenvtg amllgtrdgr
      841 sasfegglnl lkrddtpski dastntsdeg mtllatstpe elvatpeevs vgtqtavcdt
      901 dapssvagle avsrtarrls ftgsetsave eqlsletqks lpaspkskss alpvqksasp
      961 snsttkpdtr agsaglglss esttrlsskq ahsstsvhga rltagkgape dkvappslss
     1021 aakrsaaldk grilqrhgsl nptlgsrasv trapkfspar vapvpkpgps krsktasdep
     1081 rrakltplds kkfpsendpa clvaaptyrp tademrdpis yiskirpeae kfgicriilp
     1141 psfqpeckvf ddmrftaynq yihkmlsrwg pnvqhtacik rcftkqgitd dqppliggie
     1201 ldlsylyhtv qnfggiqqvm ekkkwhrvad amhipkaaqd rvskvddayc kfvlpydlls
     1261 adekkaiele vladrerqma adsepsstsa dedeeeefsn ecvakvsrhf aslgshgrmk
     1321 qppawtdqsn trkgrsmsls vfsrvarntm smwykhepst eevekdywnl vidqrrhvcv
     1381 hagnidssvh gsgfptnrlv pfakhpwnlk vltnnpgtvl rcmgpvsgvt iptlhlsmly
     1441 ttgcwyrdph clpwveylht gankiwysvp ahssdrfrsa mkeimpqvck dsaiwlpsdc
     1501 amahnrpqky qtydr
//
LOCUS       XP_077503915            1413 aa            linear   INV 25-NOV-2025
DEFINITION  jumonji, AT rich interactive domain 2 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077503915
VERSION     XP_077503915.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647789.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1413
                     /product="jumonji, AT rich interactive domain 2 isoform
                     X4"
                     /calculated_mol_wt=153442
     Region          836..>1035
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     Region          <1007..1113
                     /region_name="PRK14954"
                     /note="DNA polymerase III subunits gamma and tau;
                     Provisional"
                     /db_xref="CDD:184918"
     Region          1105..1145
                     /region_name="JmjN"
                     /note="Small domain found in the jumonji family of
                     transcription factors; smart00545"
                     /db_xref="CDD:128818"
     Region          1168..1278
                     /region_name="ARID_JARD2"
                     /note="ARID/BRIGHT DNA binding domain of Jumonji/ARID
                     domain-containing protein 2 (JARID2) and similar proteins;
                     cd16870"
                     /db_xref="CDD:350634"
     Site            order(1192..1198,1223,1225..1226,1229,1240,1242..1244,
                     1246)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:350634"
     CDS             1..1413
                     /gene="Jarid2"
                     /coded_by="XM_077647789.1:445..4686"
                     /db_xref="GeneID:144114203"
ORIGIN      
        1 mksafkerql sdysatsqrp krnastkglg csnglhwmee kqlkkalyas lnetkkarll
       61 denasedgre qapktttstv vakkaadtds krikvhaqrk faqgsnpssp mptpvkllpp
      121 cresrdtvsq pstllpckrr pktedfltfl clrgtaalpp aldfmsgtaf pgsssssdrs
      181 sspemdasla sttaeyrdrg asagalwgtv padvrttaqr aatrvpkqdr rpfrpesgee
      241 kqaseqrssr satakkmdkr kivpekskdt lkpgsiskcl skpngnltal qekykqrria
      301 lqtgpsrkqs alghdgghal rsravrggvh sgskravkss cvksreqlgs alpnrhsvsg
      361 rdeemaaknr lvlkrqlsvq ltrlhpriaa glrlpimass gtgvvtrrrs vgitphlrka
      421 kmvppivdye sesekdlspl spprspliav qprsratgsk thnasqkpks kvtvstttkk
      481 kptsviarkl lnrattrmrp tsavrkrtrc tmfrrktrsl gpvkeyplvl alptkrgagr
      541 fggtvakkka eglaakstkl kvpltrssel pqvsskassa aaqqklkkvt vfsqndgtlk
      601 rstrrvsseg tsdmrslrqr sdatatkckr ethkaiigas ektqkaiatk rrkvevsasn
      661 asshlfsdsd deplikvagr lsekkvlsat ekdchskqhk sgsaqksgvv tppvtgmlsr
      721 kkaasvkdkn klpeidnsrf fknslvsesf sepspsslse tiadaagdpl dtrgdfddle
      781 smsggveald alageisqvm scrlsfgsaq eiqslmtsef sgafdenvtg amllgtrdgr
      841 sasfegglnl lkrddtpski dastntsdeg mtllatstpe elvatpeevs vgtqtavcdt
      901 dapssvagle avsrtarrls ftgsetsave eqlsletqks lpaspkskss alpvqksasp
      961 snsttkpdtr agsaglglss esttrlsskq ahsstsvhga rltagkgape dkvappslss
     1021 aakrsaaldk grilqrhgsl nptlgsrasv trapkfspar vapvpkpgps krsktasdep
     1081 rrakltplds kkfpsendpa clvaaptyrp tademrdpis yiskirpeae kfgicriilp
     1141 psfqpeckvf ddmrftaynq yihkmlsrwg pnvqhtacik rcftkqgitd dqppliggie
     1201 ldlsylyhtv qnfggiqqvm ekkkwhrvad amhipkaaqd rvskvddayc kfvlpydlls
     1261 adekkaiele vladrerqma adsepsstsa dedeeeefsn ecvakvsrhf aslgshgrmk
     1321 qppawtdqsn trkgrsmsls vfsrvarntm smwykhepst eevekdywnl vidqrrhvcv
     1381 hagnidssvh gsgfptnrlv pfakkrkytt msr
//
LOCUS       XP_077503916             602 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylinositol glycan anchor biosynthesis class T [Amblyomma
            americanum].
ACCESSION   XP_077503916
VERSION     XP_077503916.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647790.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..602
                     /product="phosphatidylinositol glycan anchor biosynthesis
                     class T"
                     /calculated_mol_wt=66925
     Region          15..550
                     /region_name="Gpi16"
                     /note="Gpi16 subunit, GPI transamidase component;
                     pfam04113"
                     /db_xref="CDD:461180"
     CDS             1..602
                     /gene="PIG-T"
                     /coded_by="XM_077647790.1:32..1840"
                     /db_xref="GeneID:144114204"
ORIGIN      
        1 massfssclw wlcsffvvqc tlakgqhsds fteelfikpl ssghvynyfl fttrwnidra
       61 ketfqhfrlf plslgeivsq qrvqelhlsl tqgrwqhrkw gypfqeaptg ahlwawfdpd
      121 tpsvssawkn ltnalsgqlc aslnfvddtv tvspkrsfrp qgwvrsanss llryaalpre
      181 svctenltpw kkllpcsska glatllhalq lfianyisla ldlkmicqde dclhaalelq
      241 msvslvfdav aaqngyqtws lsklfgaglk ttcplasmss iyvdvsnngs vgtfwlspep
      301 tqlvvsgqga hqrslaiydl khhvaqgrfn laaqyekphi fwlipepllh stryiqgygl
      361 erggivnhir nnhptktirv vlldvvpwfl rvylhtlkvs sgsrqltaeh vsyqpgrdre
      421 rpyhleltll lppasetvva yeferaflkw teyppdanhg fyvgaavlss llddpvanys
      481 gdislcpslr hcitgtastp dakniagkqf vriytetllv slptpdfsmp ynviclactv
      541 valafgpihn matnaltive akkerggllr qlvskakgfl qkgsapdasg agdnsdedkk
      601 nk
//
LOCUS       XP_077503917             540 aa            linear   INV 25-NOV-2025
DEFINITION  calcitonin gene-related peptide type 1 receptor-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077503917
VERSION     XP_077503917.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647791.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..540
                     /product="calcitonin gene-related peptide type 1
                     receptor-like isoform X1"
                     /calculated_mol_wt=62936
     Region          164..230
                     /region_name="HRM"
                     /note="Hormone receptor domain; pfam02793"
                     /db_xref="CDD:397086"
     Region          243..510
                     /region_name="7tmB1_hormone_R"
                     /note="The subfamily B1 of hormone receptors
                     (secretin-like), member of the class B family
                     seven-transmembrane G protein-coupled receptors; cd15041"
                     /db_xref="CDD:341321"
     Region          243..267
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:341321"
     Site            order(247,251,293,296..297,300,323,330,398..399,401,403,
                     457,460,475,479)
                     /site_type="other"
                     /note="putative polypeptide ligand binding pocket
                     [polypeptide binding]"
                     /db_xref="CDD:341321"
     Region          276..298
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:341321"
     Region          323..350
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:341321"
     Region          361..381
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:341321"
     Region          395..424
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:341321"
     Region          438..465
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:341321"
     Region          472..497
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:341321"
     CDS             1..540
                     /gene="LOC144114205"
                     /coded_by="XM_077647791.1:663..2285"
                     /db_xref="GeneID:144114205"
ORIGIN      
        1 msyhssrwwa yclifafacq llllvlpgaa lefqgrhlah apegarserh venetctfrg
       61 dpytvdeftk kaavecymyt nglreeyqkk vcyktryypk iclnctdtqn aneadchlpd
      121 iddensplfr afrtkaigrl wancvreaia ccqymryapr rtdgvycnrt wdgltcyedt
      181 danrnvskvc prvlyiyskp hceyystrwc hengswyrlm nqqrtfytsc apkpwvsgey
      241 igvyhysigl ftlsvlslip alvifnwyks lqvpritihk hlcfavmmcg vmyiidhsvf
      301 tlddvkptky tknlthlntw wckllsvlnr yfmvsqytwm lcegcylhrl valafskqmn
      361 lkfcyvfgwg lpavlvtiys slratqsddr cwrnivvydy tftgpilacl avniffmvhv
      421 iyilvtklrq rnvndtenfr kilravvili pvfgvhfvlt tfvspsscsa ylkklyaewg
      481 ivglqgffvs lifcylnsei qsllkrsynr ykndrslsrr hrpsvpqttt tttmthttpm
//
LOCUS       XP_077503918             540 aa            linear   INV 25-NOV-2025
DEFINITION  calcitonin gene-related peptide type 1 receptor-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077503918
VERSION     XP_077503918.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647792.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..540
                     /product="calcitonin gene-related peptide type 1
                     receptor-like isoform X1"
                     /calculated_mol_wt=62936
     Region          164..230
                     /region_name="HRM"
                     /note="Hormone receptor domain; pfam02793"
                     /db_xref="CDD:397086"
     Region          243..510
                     /region_name="7tmB1_hormone_R"
                     /note="The subfamily B1 of hormone receptors
                     (secretin-like), member of the class B family
                     seven-transmembrane G protein-coupled receptors; cd15041"
                     /db_xref="CDD:341321"
     Region          243..267
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:341321"
     Site            order(247,251,293,296..297,300,323,330,398..399,401,403,
                     457,460,475,479)
                     /site_type="other"
                     /note="putative polypeptide ligand binding pocket
                     [polypeptide binding]"
                     /db_xref="CDD:341321"
     Region          276..298
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:341321"
     Region          323..350
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:341321"
     Region          361..381
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:341321"
     Region          395..424
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:341321"
     Region          438..465
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:341321"
     Region          472..497
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:341321"
     CDS             1..540
                     /gene="LOC144114205"
                     /coded_by="XM_077647792.1:744..2366"
                     /db_xref="GeneID:144114205"
ORIGIN      
        1 msyhssrwwa yclifafacq llllvlpgaa lefqgrhlah apegarserh venetctfrg
       61 dpytvdeftk kaavecymyt nglreeyqkk vcyktryypk iclnctdtqn aneadchlpd
      121 iddensplfr afrtkaigrl wancvreaia ccqymryapr rtdgvycnrt wdgltcyedt
      181 danrnvskvc prvlyiyskp hceyystrwc hengswyrlm nqqrtfytsc apkpwvsgey
      241 igvyhysigl ftlsvlslip alvifnwyks lqvpritihk hlcfavmmcg vmyiidhsvf
      301 tlddvkptky tknlthlntw wckllsvlnr yfmvsqytwm lcegcylhrl valafskqmn
      361 lkfcyvfgwg lpavlvtiys slratqsddr cwrnivvydy tftgpilacl avniffmvhv
      421 iyilvtklrq rnvndtenfr kilravvili pvfgvhfvlt tfvspsscsa ylkklyaewg
      481 ivglqgffvs lifcylnsei qsllkrsynr ykndrslsrr hrpsvpqttt tttmthttpm
//
LOCUS       XP_077503919             540 aa            linear   INV 25-NOV-2025
DEFINITION  calcitonin gene-related peptide type 1 receptor-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077503919
VERSION     XP_077503919.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647793.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..540
                     /product="calcitonin gene-related peptide type 1
                     receptor-like isoform X1"
                     /calculated_mol_wt=62936
     Region          164..230
                     /region_name="HRM"
                     /note="Hormone receptor domain; pfam02793"
                     /db_xref="CDD:397086"
     Region          243..510
                     /region_name="7tmB1_hormone_R"
                     /note="The subfamily B1 of hormone receptors
                     (secretin-like), member of the class B family
                     seven-transmembrane G protein-coupled receptors; cd15041"
                     /db_xref="CDD:341321"
     Region          243..267
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:341321"
     Site            order(247,251,293,296..297,300,323,330,398..399,401,403,
                     457,460,475,479)
                     /site_type="other"
                     /note="putative polypeptide ligand binding pocket
                     [polypeptide binding]"
                     /db_xref="CDD:341321"
     Region          276..298
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:341321"
     Region          323..350
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:341321"
     Region          361..381
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:341321"
     Region          395..424
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:341321"
     Region          438..465
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:341321"
     Region          472..497
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:341321"
     CDS             1..540
                     /gene="LOC144114205"
                     /coded_by="XM_077647793.1:343..1965"
                     /db_xref="GeneID:144114205"
ORIGIN      
        1 msyhssrwwa yclifafacq llllvlpgaa lefqgrhlah apegarserh venetctfrg
       61 dpytvdeftk kaavecymyt nglreeyqkk vcyktryypk iclnctdtqn aneadchlpd
      121 iddensplfr afrtkaigrl wancvreaia ccqymryapr rtdgvycnrt wdgltcyedt
      181 danrnvskvc prvlyiyskp hceyystrwc hengswyrlm nqqrtfytsc apkpwvsgey
      241 igvyhysigl ftlsvlslip alvifnwyks lqvpritihk hlcfavmmcg vmyiidhsvf
      301 tlddvkptky tknlthlntw wckllsvlnr yfmvsqytwm lcegcylhrl valafskqmn
      361 lkfcyvfgwg lpavlvtiys slratqsddr cwrnivvydy tftgpilacl avniffmvhv
      421 iyilvtklrq rnvndtenfr kilravvili pvfgvhfvlt tfvspsscsa ylkklyaewg
      481 ivglqgffvs lifcylnsei qsllkrsynr ykndrslsrr hrpsvpqttt tttmthttpm
//
LOCUS       XP_077503920             537 aa            linear   INV 25-NOV-2025
DEFINITION  calcitonin gene-related peptide type 1 receptor-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077503920
VERSION     XP_077503920.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647794.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..537
                     /product="calcitonin gene-related peptide type 1
                     receptor-like isoform X2"
                     /calculated_mol_wt=62693
     Region          164..230
                     /region_name="HRM"
                     /note="Hormone receptor domain; pfam02793"
                     /db_xref="CDD:397086"
     Region          240..507
                     /region_name="7tmB1_hormone_R"
                     /note="The subfamily B1 of hormone receptors
                     (secretin-like), member of the class B family
                     seven-transmembrane G protein-coupled receptors; cd15041"
                     /db_xref="CDD:341321"
     Region          240..264
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:341321"
     Site            order(244,248,290,293..294,297,320,327,395..396,398,400,
                     454,457,472,476)
                     /site_type="other"
                     /note="putative polypeptide ligand binding pocket
                     [polypeptide binding]"
                     /db_xref="CDD:341321"
     Region          273..295
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:341321"
     Region          320..347
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:341321"
     Region          358..378
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:341321"
     Region          392..421
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:341321"
     Region          435..462
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:341321"
     Region          469..494
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:341321"
     CDS             1..537
                     /gene="LOC144114205"
                     /coded_by="XM_077647794.1:663..2276"
                     /db_xref="GeneID:144114205"
ORIGIN      
        1 msyhssrwwa yclifafacq llllvlpgaa lefqgrhlah apegarserh venetctfrg
       61 dpytvdeftk kaavecymyt nglreeyqkk vcyktryypk iclnctdtqn aneadchlpd
      121 iddensplfr afrtkaigrl wancvreaia ccqymryapr rtdgvycnrt wdgltcyedt
      181 danrnvskvc prvlyiyskp hceyystrwc hengswyrlm nqqrtfytsc apkpweyigv
      241 yhysiglftl svlslipalv ifnwykslqv pritihkhlc favmmcgvmy iidhsvftld
      301 dvkptkytkn lthlntwwck llsvlnryfm vsqytwmlce gcylhrlval afskqmnlkf
      361 cyvfgwglpa vlvtiysslr atqsddrcwr nivvydytft gpilaclavn iffmvhviyi
      421 lvtklrqrnv ndtenfrkil ravvilipvf gvhfvlttfv spsscsaylk klyaewgivg
      481 lqgffvslif cylnseiqsl lkrsynrykn drslsrrhrp svpqtttttt mthttpm
//
LOCUS       XP_077503921             499 aa            linear   INV 25-NOV-2025
DEFINITION  calcitonin gene-related peptide type 1 receptor-like isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077503921
VERSION     XP_077503921.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647795.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..499
                     /product="calcitonin gene-related peptide type 1
                     receptor-like isoform X3"
                     /calculated_mol_wt=58121
     Region          202..469
                     /region_name="7tmB1_hormone_R"
                     /note="The subfamily B1 of hormone receptors
                     (secretin-like), member of the class B family
                     seven-transmembrane G protein-coupled receptors; cd15041"
                     /db_xref="CDD:341321"
     Region          202..226
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:341321"
     Site            order(206,210,252,255..256,259,282,289,357..358,360,362,
                     416,419,434,438)
                     /site_type="other"
                     /note="putative polypeptide ligand binding pocket
                     [polypeptide binding]"
                     /db_xref="CDD:341321"
     Region          235..257
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:341321"
     Region          282..309
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:341321"
     Region          320..340
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:341321"
     Region          354..383
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:341321"
     Region          397..424
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:341321"
     Region          431..456
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:341321"
     CDS             1..499
                     /gene="LOC144114205"
                     /coded_by="XM_077647795.1:665..2164"
                     /db_xref="GeneID:144114205"
ORIGIN      
        1 msyhssrwwa yclifafacq llllvlpgaa lefqgrhlah apegarserh venetctfrg
       61 dpytvdeftk kaavecymyt nglreeyqkk vcyktryypk iclnctdtqn aneadchlpd
      121 iddensplfr afrtkaigrl wancvreaia ccqymryapr rtdystrwch engswyrlmn
      181 qqrtfytsca pkpwvsgeyi gvyhysiglf tlsvlslipa lvifnwyksl qvpritihkh
      241 lcfavmmcgv myiidhsvft lddvkptkyt knlthlntww ckllsvlnry fmvsqytwml
      301 cegcylhrlv alafskqmnl kfcyvfgwgl pavlvtiyss lratqsddrc wrnivvydyt
      361 ftgpilacla vniffmvhvi yilvtklrqr nvndtenfrk ilravvilip vfgvhfvltt
      421 fvspsscsay lkklyaewgi vglqgffvsl ifcylnseiq sllkrsynry kndrslsrrh
      481 rpsvpqtttt ttmthttpm
//
LOCUS       XP_077503922             496 aa            linear   INV 25-NOV-2025
DEFINITION  calcitonin gene-related peptide type 1 receptor-like isoform X4
            [Amblyomma americanum].
ACCESSION   XP_077503922
VERSION     XP_077503922.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647796.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..496
                     /product="calcitonin gene-related peptide type 1
                     receptor-like isoform X4"
                     /calculated_mol_wt=57878
     Region          199..466
                     /region_name="7tmB1_hormone_R"
                     /note="The subfamily B1 of hormone receptors
                     (secretin-like), member of the class B family
                     seven-transmembrane G protein-coupled receptors; cd15041"
                     /db_xref="CDD:341321"
     Region          199..223
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:341321"
     Site            order(203,207,249,252..253,256,279,286,354..355,357,359,
                     413,416,431,435)
                     /site_type="other"
                     /note="putative polypeptide ligand binding pocket
                     [polypeptide binding]"
                     /db_xref="CDD:341321"
     Region          232..254
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:341321"
     Region          279..306
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:341321"
     Region          317..337
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:341321"
     Region          351..380
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:341321"
     Region          394..421
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:341321"
     Region          428..453
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:341321"
     CDS             1..496
                     /gene="LOC144114205"
                     /coded_by="XM_077647796.1:666..2156"
                     /db_xref="GeneID:144114205"
ORIGIN      
        1 msyhssrwwa yclifafacq llllvlpgaa lefqgrhlah apegarserh venetctfrg
       61 dpytvdeftk kaavecymyt nglreeyqkk vcyktryypk iclnctdtqn aneadchlpd
      121 iddensplfr afrtkaigrl wancvreaia ccqymryapr rtdystrwch engswyrlmn
      181 qqrtfytsca pkpweyigvy hysiglftls vlslipalvi fnwykslqvp ritihkhlcf
      241 avmmcgvmyi idhsvftldd vkptkytknl thlntwwckl lsvlnryfmv sqytwmlceg
      301 cylhrlvala fskqmnlkfc yvfgwglpav lvtiysslra tqsddrcwrn ivvydytftg
      361 pilaclavni ffmvhviyil vtklrqrnvn dtenfrkilr avvilipvfg vhfvlttfvs
      421 psscsaylkk lyaewgivgl qgffvslifc ylnseiqsll krsynryknd rslsrrhrps
      481 vpqttttttm thttpm
//
LOCUS       XP_077503924             458 aa            linear   INV 25-NOV-2025
DEFINITION  calcitonin receptor-like isoform X5 [Amblyomma americanum].
ACCESSION   XP_077503924
VERSION     XP_077503924.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647798.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..458
                     /product="calcitonin receptor-like isoform X5"
                     /calculated_mol_wt=53416
     Region          164..230
                     /region_name="HRM"
                     /note="Hormone receptor domain; pfam02793"
                     /db_xref="CDD:397086"
     Region          243..>440
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          243..267
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410628"
     Region          276..297
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410628"
     Region          323..345
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410628"
     Region          363..379
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410628"
     Region          395..418
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410628"
     CDS             1..458
                     /gene="LOC144114205"
                     /coded_by="XM_077647798.1:666..2042"
                     /db_xref="GeneID:144114205"
ORIGIN      
        1 msyhssrwwa yclifafacq llllvlpgaa lefqgrhlah apegarserh venetctfrg
       61 dpytvdeftk kaavecymyt nglreeyqkk vcyktryypk iclnctdtqn aneadchlpd
      121 iddensplfr afrtkaigrl wancvreaia ccqymryapr rtdgvycnrt wdgltcyedt
      181 danrnvskvc prvlyiyskp hceyystrwc hengswyrlm nqqrtfytsc apkpwvsgey
      241 igvyhysigl ftlsvlslip alvifnwyks lqvpritihk hlcfavmmcg vmyiidhsvf
      301 tlddvkptky tknlthlntw wckllsvlnr yfmvsqytwm lcegcylhrl valafskqmn
      361 lkfcyvfgwg lpavlvtiys slratqsddr cwrnivvydy tftgpilacl avniffmvhv
      421 iyilvtklrq rnvndtenfr afsfpsysat starsrac
//
LOCUS       XP_077503925             595 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114206 [Amblyomma americanum].
ACCESSION   XP_077503925
VERSION     XP_077503925.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647799.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..595
                     /product="uncharacterized protein LOC144114206"
                     /calculated_mol_wt=65852
     CDS             1..595
                     /gene="LOC144114206"
                     /coded_by="XM_077647799.1:81..1868"
                     /db_xref="GeneID:144114206"
ORIGIN      
        1 maedpfltsh gassghhley trgcrviena dhsmelveac pkvnltclfq cayqvlkans
       61 pasdlpvvvg lnkqdeniva fcqenayniv seigaaidkc llqalgkdvs aaglyglafv
      121 rcgeledaaf itfvksnqlv irylvsfkqg massfadvln aklqklgvad scrlvaatvd
      181 rdcfhveeie allnakkdrs nallefhcvi yklesvlgha lqksmlddpq vaiianlcnm
      241 lknlaladdl nlgdamfqgp esststffny ktlvtlhnhw ldivtklnkv lvdslveddr
      301 qvisfilatl ksttfivnla fyielfrslh ewtsrvvalg ipayqvlnel qgasstlrhh
      361 tatggvrvee lleelrqscg ryrgvdiscd ldealftatm nkvcslmaei lddivrqtsp
      421 sirkfsildp kvwpvdtcql ddfgfdsika lareyrysll dnvdvfqewg sykrtvqnsf
      481 vtqlrdnfda lavrmvssfk slypgivtll savtllcpas kalqkntqll legeqseevr
      541 niqingppvg swsaasalsf llhercveqt tqfsdktcdl mlpmdivrsm fvtta
//
LOCUS       XP_077503926             147 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114210 [Amblyomma americanum].
ACCESSION   XP_077503926
VERSION     XP_077503926.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647800.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..147
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..147
                     /product="uncharacterized protein LOC144114210"
                     /calculated_mol_wt=16044
     CDS             1..147
                     /gene="LOC144114210"
                     /coded_by="XM_077647800.1:1265..1708"
                     /db_xref="GeneID:144114210"
ORIGIN      
        1 mslssgnrlq istrkaylih dctgstlgts phqtrscqpr slrsgrcrrc fssnihqspc
       61 paaadpldsc ritdwmpccs wrllwstshp llmeavdtit csdtagalfp sltnvargar
      121 ippglgfskt ekktkwiyns ekangeq
//
LOCUS       XP_077503927             350 aa            linear   INV 25-NOV-2025
DEFINITION  regulation of nuclear pre-mRNA domain-containing protein 1B isoform
            X1 [Amblyomma americanum].
ACCESSION   XP_077503927
VERSION     XP_077503927.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647801.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..350
                     /product="regulation of nuclear pre-mRNA domain-containing
                     protein 1B isoform X1"
                     /calculated_mol_wt=39217
     Region          4..130
                     /region_name="CID_RPRD1"
                     /note="CID (CTD-Interacting Domain) of Regulation of
                     nuclear pre-mRNA domain-containing protein 1 and similar
                     proteins; cd17002"
                     /db_xref="CDD:340799"
     Site            order(18..20,23,61,64..65,68..69,107,111,114..115)
                     /site_type="other"
                     /note="CTD binding site [polypeptide binding]"
                     /db_xref="CDD:340799"
     Region          204..348
                     /region_name="CREPT"
                     /note="Cell-cycle alteration and expression-elevated
                     protein in tumour; pfam16566"
                     /db_xref="CDD:465181"
     CDS             1..350
                     /gene="LOC144114211"
                     /coded_by="XM_077647801.1:119..1171"
                     /db_xref="GeneID:144114211"
ORIGIN      
        1 magftesale rklaelnnsq qsiqtlslwl ihhrkhhkti vhvwlrelkk akparkltfm
       61 ylandviqns kkkgpeftke fatvlakafa hisvrdtdqk thaglrrils vwkersvfds
      121 hqisdfgqyl sdkvaetdav kdtvysgrkg hapisregtp lwndpepvvk rkkpsdssee
      181 tsegtdgdkd aekkpgeppd pgvlikaltd lentassdav vreriaslpp evsdasllek
      241 iqdrqaadrl saqveeacsl laeynnrltq eledrkqvsq mlanftfhqr elmalaeqkl
      301 eehrdklhkv thvrnelksh lqnlpdltll psvtgglapl psagdlfshq
//
LOCUS       XP_077503928             309 aa            linear   INV 25-NOV-2025
DEFINITION  regulation of nuclear pre-mRNA domain-containing protein 1B isoform
            X2 [Amblyomma americanum].
ACCESSION   XP_077503928
VERSION     XP_077503928.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647802.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..309
                     /product="regulation of nuclear pre-mRNA domain-containing
                     protein 1B isoform X2"
                     /calculated_mol_wt=34274
     Region          <10..89
                     /region_name="VHS_ENTH_ANTH"
                     /note="VHS, ENTH and ANTH domain superfamily; cl02544"
                     /db_xref="CDD:470608"
     Region          163..307
                     /region_name="CREPT"
                     /note="Cell-cycle alteration and expression-elevated
                     protein in tumour; pfam16566"
                     /db_xref="CDD:465181"
     CDS             1..309
                     /gene="LOC144114211"
                     /coded_by="XM_077647802.1:44..973"
                     /db_xref="GeneID:144114211"
ORIGIN      
        1 mcllhstipa kparkltfmy landviqnsk kkgpeftkef atvlakafah isvrdtdqkt
       61 haglrrilsv wkersvfdsh qisdfgqyls dkvaetdavk dtvysgrkgh apisregtpl
      121 wndpepvvkr kkpsdsseet segtdgdkda ekkpgeppdp gvlikaltdl entassdavv
      181 reriaslppe vsdaslleki qdrqaadrls aqveeacsll aeynnrltqe ledrkqvsqm
      241 lanftfhqre lmalaeqkle ehrdklhkvt hvrnelkshl qnlpdltllp svtgglaplp
      301 sagdlfshq
//
LOCUS       XP_077503929             201 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase ZNRF2 [Amblyomma americanum].
ACCESSION   XP_077503929
VERSION     XP_077503929.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647803.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..201
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..201
                     /product="E3 ubiquitin-protein ligase ZNRF2"
                     /calculated_mol_wt=20965
     Region          146..201
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(158,161,176,178,181,184,195,198)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     CDS             1..201
                     /gene="LOC144114212"
                     /coded_by="XM_077647803.1:287..892"
                     /db_xref="GeneID:144114212"
ORIGIN      
        1 mggkqsttts nprvsagsgd ttgaaaataa aggglssllt gahhsssstd arlrarslss
       61 vlnlhpgqpl sipasqsgfg tpesppesgs stpdstpfgr vfaahslpvq lvsfngikcp
      121 vcskfvlpdd vechlvmclt kprisynddv ltedkgecvi cleelnrgdt iarlpclciy
      181 hkrcidawfe vnrscpehpl d
//
LOCUS       XP_077503930             164 aa            linear   INV 25-NOV-2025
DEFINITION  magnesium-dependent phosphatase 1-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503930
VERSION     XP_077503930.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647804.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..164
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..164
                     /product="magnesium-dependent phosphatase 1-like isoform
                     X1"
                     /calculated_mol_wt=19050
     Region          9..163
                     /region_name="Acid_PPase"
                     /note="Acid Phosphatase; pfam12689"
                     /db_xref="CDD:372256"
     Site            order(15..19,72..73,103,125..126,129..130)
                     /site_type="active"
                     /db_xref="CDD:319804"
     CDS             1..164
                     /gene="LOC144114213"
                     /coded_by="XM_077647804.1:211..705"
                     /db_xref="GeneID:144114213"
ORIGIN      
        1 mcrtdtkrpk livfdldytl wpfyvdthvs ppfkkekgki vdrhgrkvdv ypdvpamlqn
       61 lksqnyqmgv asrtddpdaa rqlltildwn kyfpfqeiyp gckvthfnkf aqrtgfsykq
      121 mlffddemrn itdvsklgvt svyvkdgmsq evideglsrf enet
//
LOCUS       XP_077503931             150 aa            linear   INV 25-NOV-2025
DEFINITION  magnesium-dependent phosphatase 1-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503931
VERSION     XP_077503931.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647805.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..150
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..150
                     /product="magnesium-dependent phosphatase 1-like isoform
                     X2"
                     /calculated_mol_wt=17565
     Region          9..145
                     /region_name="Acid_PPase"
                     /note="Acid Phosphatase; pfam12689"
                     /db_xref="CDD:372256"
     Site            order(15..19,72..73,103,125..126,129..130)
                     /site_type="active"
                     /db_xref="CDD:319804"
     CDS             1..150
                     /gene="LOC144114213"
                     /coded_by="XM_077647805.1:148..600"
                     /db_xref="GeneID:144114213"
ORIGIN      
        1 mcrtdtkrpk livfdldytl wpfyvdthvs ppfkkekgki vdrhgrkvdv ypdvpamlqn
       61 lksqnyqmgv asrtddpdaa rqlltildwn kyfpfqeiyp gckvthfnkf aqrtgfsykq
      121 mlffddemrn itdvsklafq strrgqrslv
//
LOCUS       XP_077503932             279 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger protein 532-like [Amblyomma americanum].
ACCESSION   XP_077503932
VERSION     XP_077503932.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647806.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..279
                     /product="zinc finger protein 532-like"
                     /calculated_mol_wt=31423
     CDS             1..279
                     /gene="LOC144114214"
                     /coded_by="XM_077647806.1:132..971"
                     /db_xref="GeneID:144114214"
ORIGIN      
        1 massktvrfl lppkvfpamt prkrvpehck crfkcptcea vfshyvllcv htwshapgdf
       61 rvpdpavrpc silqstenfg lqcflcplrf gshlalrnhl dashtaaddv kllrfacafc
      121 amryasadll iahiqqhdvw edgdfyplfa givgkplcer ddtvssfeld dlikelqkia
      181 gdlppqqris ksalskksrg gvntplnylc alcgmkfqhg yqidehvrlr hkgflklmka
      241 rhacvycgsk ffklsvlreh llmhhaisvt prkkgpvrl
//
LOCUS       XP_077503933             177 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein KIAA1143 homolog isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503933
VERSION     XP_077503933.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647807.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..177
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..177
                     /product="uncharacterized protein KIAA1143 homolog isoform
                     X1"
                     /calculated_mol_wt=19581
     Region          44..172
                     /region_name="DUF4604"
                     /note="Domain of unknown function (DUF4604); pfam15377"
                     /db_xref="CDD:464685"
     CDS             1..177
                     /gene="LOC144114215"
                     /coded_by="XM_077647807.1:1..534"
                     /db_xref="GeneID:144114215"
ORIGIN      
        1 mmmmmmvlpt nsqnsdssqe psaklrdrvs gehgsarerm pkknsvtcvk psdppflrkl
       61 kervgykegp tvdtkreelp adavavdfdd ekpvvvvlka gdltaeeaek crqkeedigs
      121 sdgriifkkp tkrtdstppa agqakrlkea dksrrdqvss ikdsrllsfg ddeeded
//
LOCUS       XP_077503934             138 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein KIAA1143 homolog isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503934
VERSION     XP_077503934.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647808.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..138
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..138
                     /product="uncharacterized protein KIAA1143 homolog isoform
                     X2"
                     /calculated_mol_wt=15216
     Region          5..>109
                     /region_name="DUF4604"
                     /note="Domain of unknown function (DUF4604); pfam15377"
                     /db_xref="CDD:464685"
     CDS             1..138
                     /gene="LOC144114215"
                     /coded_by="XM_077647808.1:124..540"
                     /db_xref="GeneID:144114215"
ORIGIN      
        1 mpkknsvtcv kpsdppflrk lkervgykeg ptvdtkreel padavavdfd dekpvvvvlk
       61 agdltaeeae kcrqkeedig ssdgriifkk ptkrtdstpp aagqakrlke adksrrdqvs
      121 sikdsrllsf gddeeded
//
LOCUS       XP_077503935             866 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger protein MEP-1 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503935
VERSION     XP_077503935.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647809.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..866
                     /product="zinc finger protein MEP-1 isoform X1"
                     /calculated_mol_wt=94742
     CDS             1..866
                     /gene="MEP-1"
                     /coded_by="XM_077647809.1:221..2821"
                     /db_xref="GeneID:144114216"
ORIGIN      
        1 mvttekentn esmpsstsqr pgenthkssa ekmegatmnt kaeedsiasq pndsndndsn
       61 sseqgrngvs edsrvpskdp qntsenktsk dtpeqpeqde sedekedeea kirpglpkfl
      121 rryvkigkyv pvtmpksgdf rmeelealle apslvtplli kkpdqflten gaarekkpkk
      181 erapslgkgg nsvadyfast agefliglgl sralevfnrd lirlkeremk kaggrtqemi
      241 eehrrlteah qqakianapy tfkvkpcrvc dfktesslvl eghlltphft srrelqcsfc
      301 nfvtrdakai vfhmeaihnr lptmepppqf yecpfcpfet nlktkasghv nrcqkyfnng
      361 mnqlpsgdfq ppgvtakpit iedirtyekf fmslgsnata pvkgtqnrty aalsrngapa
      421 lagprgaapp lyqqlasaas lnqnrgrgrp rlaliqpapv snqvfqvvgs gsevipllng
      481 spqltvtlar apapsnngnn trpapraaap lpaqqqappg qrprtpvile ssgsgaafvi
      541 ceicdgyikd leqlrthmqw ihkvkihpkm lasrpplncq kcqwrfftdq glerhllgah
      601 glvtsnmqdl ansgqdggrc tvcgriyask lvahmsqvhr vvlkpahlsy kctvctatfn
      661 lyklfethvy tvhsgamkrs sssaasderp akrpavapps aplapakaip akaepkpetk
      721 evatapvkpv pkppvsgskv eevaptkvap aapvepaeps lgsrcstcke svedlaehlk
      781 ekhmrrchvr vchiekcdrc lcsfdgmivl htsdfgeeds seedeedeed agrpepmdvd
      841 gpdcakdtag ldsrkqdvdg qqevge
//
LOCUS       XP_077503936             865 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger protein MEP-1 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503936
VERSION     XP_077503936.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647810.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..865
                     /product="zinc finger protein MEP-1 isoform X2"
                     /calculated_mol_wt=94655
     CDS             1..865
                     /gene="MEP-1"
                     /coded_by="XM_077647810.1:221..2818"
                     /db_xref="GeneID:144114216"
ORIGIN      
        1 mvttekentn esmpsstsqr pgenthksae kmegatmntk aeedsiasqp ndsndndsns
       61 seqgrngvse dsrvpskdpq ntsenktskd tpeqpeqdes edekedeeak irpglpkflr
      121 ryvkigkyvp vtmpksgdfr meeleallea pslvtpllik kpdqflteng aarekkpkke
      181 rapslgkggn svadyfasta gefliglgls ralevfnrdl irlkeremkk aggrtqemie
      241 ehrrlteahq qakianapyt fkvkpcrvcd fktesslvle ghlltphfts rrelqcsfcn
      301 fvtrdakaiv fhmeaihnrl ptmepppqfy ecpfcpfetn lktkasghvn rcqkyfnngm
      361 nqlpsgdfqp pgvtakpiti edirtyekff mslgsnatap vkgtqnrtya alsrngapal
      421 agprgaappl yqqlasaasl nqnrgrgrpr laliqpapvs nqvfqvvgsg sevipllngs
      481 pqltvtlara papsnngnnt rpapraaapl paqqqappgq rprtpviles sgsgaafvic
      541 eicdgyikdl eqlrthmqwi hkvkihpkml asrpplncqk cqwrfftdqg lerhllgahg
      601 lvtsnmqdla nsgqdggrct vcgriyaskl vahmsqvhrv vlkpahlsyk ctvctatfnl
      661 yklfethvyt vhsgamkrss ssaasderpa krpavappsa plapakaipa kaepkpetke
      721 vatapvkpvp kppvsgskve evaptkvapa apvepaepsl gsrcstckes vedlaehlke
      781 khmrrchvrv chiekcdrcl csfdgmivlh tsdfgeedss eedeedeeda grpepmdvdg
      841 pdcakdtagl dsrkqdvdgq qevge
//
LOCUS       XP_077503937             866 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger protein MEP-1 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503937
VERSION     XP_077503937.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647811.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..866
                     /product="zinc finger protein MEP-1 isoform X1"
                     /calculated_mol_wt=94742
     CDS             1..866
                     /gene="MEP-1"
                     /coded_by="XM_077647811.1:320..2920"
                     /db_xref="GeneID:144114216"
ORIGIN      
        1 mvttekentn esmpsstsqr pgenthkssa ekmegatmnt kaeedsiasq pndsndndsn
       61 sseqgrngvs edsrvpskdp qntsenktsk dtpeqpeqde sedekedeea kirpglpkfl
      121 rryvkigkyv pvtmpksgdf rmeelealle apslvtplli kkpdqflten gaarekkpkk
      181 erapslgkgg nsvadyfast agefliglgl sralevfnrd lirlkeremk kaggrtqemi
      241 eehrrlteah qqakianapy tfkvkpcrvc dfktesslvl eghlltphft srrelqcsfc
      301 nfvtrdakai vfhmeaihnr lptmepppqf yecpfcpfet nlktkasghv nrcqkyfnng
      361 mnqlpsgdfq ppgvtakpit iedirtyekf fmslgsnata pvkgtqnrty aalsrngapa
      421 lagprgaapp lyqqlasaas lnqnrgrgrp rlaliqpapv snqvfqvvgs gsevipllng
      481 spqltvtlar apapsnngnn trpapraaap lpaqqqappg qrprtpvile ssgsgaafvi
      541 ceicdgyikd leqlrthmqw ihkvkihpkm lasrpplncq kcqwrfftdq glerhllgah
      601 glvtsnmqdl ansgqdggrc tvcgriyask lvahmsqvhr vvlkpahlsy kctvctatfn
      661 lyklfethvy tvhsgamkrs sssaasderp akrpavapps aplapakaip akaepkpetk
      721 evatapvkpv pkppvsgskv eevaptkvap aapvepaeps lgsrcstcke svedlaehlk
      781 ekhmrrchvr vchiekcdrc lcsfdgmivl htsdfgeeds seedeedeed agrpepmdvd
      841 gpdcakdtag ldsrkqdvdg qqevge
//
LOCUS       XP_077503938             866 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger protein MEP-1 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503938
VERSION     XP_077503938.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647812.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..866
                     /product="zinc finger protein MEP-1 isoform X1"
                     /calculated_mol_wt=94742
     CDS             1..866
                     /gene="MEP-1"
                     /coded_by="XM_077647812.1:129..2729"
                     /db_xref="GeneID:144114216"
ORIGIN      
        1 mvttekentn esmpsstsqr pgenthkssa ekmegatmnt kaeedsiasq pndsndndsn
       61 sseqgrngvs edsrvpskdp qntsenktsk dtpeqpeqde sedekedeea kirpglpkfl
      121 rryvkigkyv pvtmpksgdf rmeelealle apslvtplli kkpdqflten gaarekkpkk
      181 erapslgkgg nsvadyfast agefliglgl sralevfnrd lirlkeremk kaggrtqemi
      241 eehrrlteah qqakianapy tfkvkpcrvc dfktesslvl eghlltphft srrelqcsfc
      301 nfvtrdakai vfhmeaihnr lptmepppqf yecpfcpfet nlktkasghv nrcqkyfnng
      361 mnqlpsgdfq ppgvtakpit iedirtyekf fmslgsnata pvkgtqnrty aalsrngapa
      421 lagprgaapp lyqqlasaas lnqnrgrgrp rlaliqpapv snqvfqvvgs gsevipllng
      481 spqltvtlar apapsnngnn trpapraaap lpaqqqappg qrprtpvile ssgsgaafvi
      541 ceicdgyikd leqlrthmqw ihkvkihpkm lasrpplncq kcqwrfftdq glerhllgah
      601 glvtsnmqdl ansgqdggrc tvcgriyask lvahmsqvhr vvlkpahlsy kctvctatfn
      661 lyklfethvy tvhsgamkrs sssaasderp akrpavapps aplapakaip akaepkpetk
      721 evatapvkpv pkppvsgskv eevaptkvap aapvepaeps lgsrcstcke svedlaehlk
      781 ekhmrrchvr vchiekcdrc lcsfdgmivl htsdfgeeds seedeedeed agrpepmdvd
      841 gpdcakdtag ldsrkqdvdg qqevge
//
LOCUS       XP_077503939             480 aa            linear   INV 25-NOV-2025
DEFINITION  sodium-coupled neutral amino acid transporter 7-like [Amblyomma
            americanum].
ACCESSION   XP_077503939
VERSION     XP_077503939.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647813.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..480
                     /product="sodium-coupled neutral amino acid transporter
                     7-like"
                     /calculated_mol_wt=52212
     Region          45..445
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     CDS             1..480
                     /gene="LOC144114217"
                     /coded_by="XM_077647813.1:286..1728"
                     /db_xref="GeneID:144114217"
ORIGIN      
        1 msasqvvpey dvkfiassvn tpgagnvqds sstssssnss kgsswvsavf liinaalgag
       61 llnfphafdq aggiavalsv qavlllfvvg allvlaycar rhdcatyqev vlrvcghrfw
      121 sccslavaly cygtcitflv ivgdfsdrif aslygpsycl hwfmhrdfvi vttsvvlilp
      181 mcfsqkidfl kypsmfgvfa alylvllvvv qyfvgdynsh gartapdsle dvltavpvvc
      241 fgyqchvssv piyscledrr lstfaksvls atlltallyt vagvfgyltf ghgvasdile
      301 lydarqplvl vgilamalki ittypilifc grtavddlyg qlrgldedak rksellrrta
      361 ivsswfsttv vlaclvpnis ivirmigsla atfififpgl cllfvsvkld nsyyfaktkw
      421 klatavlcva lgtfvfgqvl tmsimdivlg hgraadvqlc tvpkterlfs yftrstlsdw
//
LOCUS       XP_077503940             746 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114219 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503940
VERSION     XP_077503940.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647814.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..746
                     /product="uncharacterized protein LOC144114219 isoform X1"
                     /calculated_mol_wt=82200
     Region          316..694
                     /region_name="CDC3"
                     /note="Septin family protein [Cell cycle control, cell
                     division, chromosome partitioning, Cytoskeleton]; COG5019"
                     /db_xref="CDD:227352"
     CDS             1..746
                     /gene="LOC144114219"
                     /coded_by="XM_077647814.1:16..2256"
                     /db_xref="GeneID:144114219"
ORIGIN      
        1 mgarlaaras pavifvhard vctarevfgp lrsalfiati dgrrerctga watslrllrs
       61 rapalrgrpi vprrvwgrlr qaavgslvpp regaasrrmt glvfsagrgg srgccsassp
      121 lsslahfpaw fqffgappsp lssvfgfprc sasrsdksfl pgappgsvps sepaaaasaw
      181 ampalprrsa ssgaadvppa rlgyryagle rfsgrprvyd spsssrsssp pplpdrqppp
      241 lqpsiapltl asslsskrel ffrdgpvppa apsagsgllg lgkdsagsla sgggggtapp
      301 ssytssapgn reaaegyvgf aslpnqvyrk avkkgfeftl mvvgeaglgk stlinsmflt
      361 diysneypgp shrvkktvev ettrvllkeg svnltltivd tpgfgdavdn tqcwqpiidy
      421 ieakyaeyld aesrvhrttl pdnrvhcciy fiapsghglk pldiefmkrl hdkvniipvi
      481 akadtmtpee cslfkrtiln eitqnkirly efpdpedeee nklqkplker vpfavvgsnt
      541 vvevngrrvr grkypwgvae venmdhcdyi alrnmlvrth mqdlkditnn vhyenyrcrk
      601 lagvggagdp ghgrsnknpl aqmeeekkdh eakmqrmeke meqvfemkvr ekmqklkese
      661 adlqrrheqm kksleqqkle leekrrnfdk ekssfeiahr dmedvlrkma vdsnskelrr
      721 svsedhfssr kcmqyaapvr knckqm
//
LOCUS       XP_077503941             733 aa            linear   INV 25-NOV-2025
DEFINITION  protein peanut-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503941
VERSION     XP_077503941.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647815.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..733
                     /product="protein peanut-like isoform X2"
                     /calculated_mol_wt=80675
     Region          316..694
                     /region_name="CDC3"
                     /note="Septin family protein [Cell cycle control, cell
                     division, chromosome partitioning, Cytoskeleton]; COG5019"
                     /db_xref="CDD:227352"
     CDS             1..733
                     /gene="LOC144114219"
                     /coded_by="XM_077647815.1:16..2217"
                     /db_xref="GeneID:144114219"
ORIGIN      
        1 mgarlaaras pavifvhard vctarevfgp lrsalfiati dgrrerctga watslrllrs
       61 rapalrgrpi vprrvwgrlr qaavgslvpp regaasrrmt glvfsagrgg srgccsassp
      121 lsslahfpaw fqffgappsp lssvfgfprc sasrsdksfl pgappgsvps sepaaaasaw
      181 ampalprrsa ssgaadvppa rlgyryagle rfsgrprvyd spsssrsssp pplpdrqppp
      241 lqpsiapltl asslsskrel ffrdgpvppa apsagsgllg lgkdsagsla sgggggtapp
      301 ssytssapgn reaaegyvgf aslpnqvyrk avkkgfeftl mvvgeaglgk stlinsmflt
      361 diysneypgp shrvkktvev ettrvllkeg svnltltivd tpgfgdavdn tqcwqpiidy
      421 ieakyaeyld aesrvhrttl pdnrvhcciy fiapsghglk pldiefmkrl hdkvniipvi
      481 akadtmtpee cslfkrtiln eitqnkirly efpdpedeee nklqkplker vpfavvgsnt
      541 vvevngrrvr grkypwgvae venmdhcdyi alrnmlvrth mqdlkditnn vhyenyrcrk
      601 lagvggagdp ghgrsnknpl aqmeeekkdh eakmqrmeke meqvfemkvr ekmqklkese
      661 adlqrrheqm kksleqqkle leekrrnfdk ekssfeiahr dmedvlrkma vdsnskeqld
      721 gkekrkekkk glf
//
LOCUS       XP_077503942             500 aa            linear   INV 25-NOV-2025
DEFINITION  septin-7-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077503942
VERSION     XP_077503942.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647816.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..500
                     /product="septin-7-like isoform X3"
                     /calculated_mol_wt=56330
     Region          89..357
                     /region_name="Septin"
                     /note="pfam00735"
                     /db_xref="CDD:395596"
     Site            98..105
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206649"
     Site            order(100..106,154,157,235..236,238,291..292)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206649"
     Site            129..137
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206649"
     Site            131
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206649"
     Site            154..157
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206649"
     Site            order(156..193,194..201)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206649"
     Site            235..238
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206649"
     Site            291..293
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206649"
     Region          <368..>488
                     /region_name="DUF5401"
                     /note="Family of unknown function (DUF5401); pfam17380"
                     /db_xref="CDD:375164"
     CDS             1..500
                     /gene="LOC144114219"
                     /coded_by="XM_077647816.1:134..1636"
                     /db_xref="GeneID:144114219"
ORIGIN      
        1 mllapavlss krelffrdgp vppaapsags gllglgkdsa gslasggggg tappssytss
       61 apgnreaaeg yvgfaslpnq vyrkavkkgf eftlmvvgea glgkstlins mfltdiysne
      121 ypgpshrvkk tvevettrvl lkegsvnltl tivdtpgfgd avdntqcwqp iidyieakya
      181 eyldaesrvh rttlpdnrvh cciyfiapsg hglkpldief mkrlhdkvni ipviakadtm
      241 tpeecslfkr tilneitqnk irlyefpdpe deeenklqkp lkervpfavv gsntvvevng
      301 rrvrgrkypw gvaevenmdh cdyialrnml vrthmqdlkd itnnvhyeny rcrklagvgg
      361 agdpghgrsn knplaqmeee kkdheakmqr mekemeqvfe mkvrekmqkl keseadlqrr
      421 heqmkksleq qkleleekrr nfdkekssfe iahrdmedvl rkmavdsnsk elrrsvsedh
      481 fssrkcmqya apvrknckqm
//
LOCUS       XP_077503943             487 aa            linear   INV 25-NOV-2025
DEFINITION  septin-7-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077503943
VERSION     XP_077503943.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647817.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..487
                     /product="septin-7-like isoform X4"
                     /calculated_mol_wt=54806
     Region          89..357
                     /region_name="Septin"
                     /note="pfam00735"
                     /db_xref="CDD:395596"
     Site            98..105
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206649"
     Site            order(100..106,154,157,235..236,238,291..292)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206649"
     Site            129..137
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206649"
     Site            131
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206649"
     Site            154..157
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206649"
     Site            order(156..193,194..201)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206649"
     Site            235..238
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206649"
     Site            291..293
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206649"
     Region          375..>462
                     /region_name="PRK12704"
                     /note="phosphodiesterase; Provisional"
                     /db_xref="CDD:237177"
     CDS             1..487
                     /gene="LOC144114219"
                     /coded_by="XM_077647817.1:133..1596"
                     /db_xref="GeneID:144114219"
ORIGIN      
        1 mllapavlss krelffrdgp vppaapsags gllglgkdsa gslasggggg tappssytss
       61 apgnreaaeg yvgfaslpnq vyrkavkkgf eftlmvvgea glgkstlins mfltdiysne
      121 ypgpshrvkk tvevettrvl lkegsvnltl tivdtpgfgd avdntqcwqp iidyieakya
      181 eyldaesrvh rttlpdnrvh cciyfiapsg hglkpldief mkrlhdkvni ipviakadtm
      241 tpeecslfkr tilneitqnk irlyefpdpe deeenklqkp lkervpfavv gsntvvevng
      301 rrvrgrkypw gvaevenmdh cdyialrnml vrthmqdlkd itnnvhyeny rcrklagvgg
      361 agdpghgrsn knplaqmeee kkdheakmqr mekemeqvfe mkvrekmqkl keseadlqrr
      421 heqmkksleq qkleleekrr nfdkekssfe iahrdmedvl rkmavdsnsk eqldgkekrk
      481 ekkkglf
//
LOCUS       XP_077503945             392 aa            linear   INV 25-NOV-2025
DEFINITION  F-box only protein 31-like [Amblyomma americanum].
ACCESSION   XP_077503945
VERSION     XP_077503945.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647819.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..392
                     /product="F-box only protein 31-like"
                     /calculated_mol_wt=44430
     Region          5..48
                     /region_name="F-box_SF"
                     /note="F-box domain superfamily; cl45894"
                     /db_xref="CDD:459239"
     Site            order(5,7..9,12..13,16,27..29,31,34)
                     /site_type="other"
                     /note="F-box motif"
                     /db_xref="CDD:438852"
     Site            order(8,11..12,15..16,20,22..23,27..29,31)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438852"
     Region          222..390
                     /region_name="Cyclin_D1_bind"
                     /note="Cyclin D1 binding domain; pfam12014"
                     /db_xref="CDD:463431"
     CDS             1..392
                     /gene="LOC144114220"
                     /coded_by="XM_077647819.1:16..1194"
                     /db_xref="GeneID:144114220"
ORIGIN      
        1 meafppevla kifsylsgkd iagvaevcrs fyeasrienv wrarcaqefa vkvkhggnli
       61 fkdvytkllh kyryclglwq pqvssygglf qvmfdpelvs ivgtelmppl dpfiteplrk
      121 kmlfsipvyr endedvicvg gykgpheavi leiskdefmf kccdsshhrh pngkhqelre
      181 flsehssvpl dmhqfphsqe mllmkflilr qfdyafhykr vrlqapltgv piqpgifkgt
      241 ygthgveliq ieyidnctkl ratklsgdpn ipsgqvtfev vlqysmvlta qqqasitsld
      301 aievqaadip cnsvpiasqp fcvpgdcher fheipkvcia ryhglgqvag hgftnpsfsr
      361 ghwvifsedl fgflwlells lsmyhrvked la
//
LOCUS       XP_077503946             864 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylinositol glycan anchor biosynthesis class O [Amblyomma
            americanum].
ACCESSION   XP_077503946
VERSION     XP_077503946.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647820.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..864
                     /product="phosphatidylinositol glycan anchor biosynthesis
                     class O"
                     /calculated_mol_wt=94096
     Region          62..341
                     /region_name="GPI_EPT_3"
                     /note="GPI ethanolamine phosphate transferase 3, PIG-O;
                     cd16023"
                     /db_xref="CDD:293747"
     Site            order(74,125,230,272..273)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:293747"
     Site            order(74,125,230,273)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:293747"
     CDS             1..864
                     /gene="PIG-O"
                     /coded_by="XM_077647820.1:283..2877"
                     /db_xref="GeneID:144114222"
ORIGIN      
        1 myrsvalavg lgiavcgvcl fccgfllqrq aveehsqcsd kevfyrssge sttlssvhcw
       61 npakfkkavi llidalklef tlygppwden eyyrnkmpif selsantgpe rsalfkfvad
      121 aptttvqrlk almtgglptf idagtnfyqt evrednlvyq lfsigkkiif mgddawvnlf
      181 pgtfaraypy psfvvkdlht vdngvlehly pelenpdkwd vlvahflgvd hcghwlgrnh
      241 pamaakltql devirsvask ltedtllvvm sdhgmtidgd hggetedeve avlflhakkp
      301 fltldskpdv tnvpsvaqvd lvptlamlld vpipysnlgs lllqafpdvs akhagpvqll
      361 plwlnvqqva rylgkvpntp lrpelrlqel qqlwdrcvgg khspeaarif vnaslvflqd
      421 arhaaqevwa tfdiprmyag laitvlgsll llcfcygqtg gksiydllrs payvcavcwl
      481 lmpfsnsfsv aehkvvlflf qtllvasvls kgsvtlntsr nrvlafclvg tlaatrfsal
      541 fwrcreehlg lpceenvlqk npsepqawgl ervlgasgca lllawgawpp tassglgaal
      601 arvglmaatg allahwfiql kptatiqgvl gshqellpnt amvvsialav lgwalphasp
      661 sylgllpasa llflltlage syavplclea aalwgltqlw ssmppsdvwg pamswlllgq
      721 lgffgtghqt sfstihwkaa fvgapidqpp mtlgafkvll ntfagplvaa aslpvmqrll
      781 stqvsccark eilplaayct llvlqvcstt asclllrrhl mvwsifaprl vfqvlsagfs
      841 ivaaiggciv srrtvirtqv lhvd
//
LOCUS       XP_077503947             302 aa            linear   INV 25-NOV-2025
DEFINITION  cyclin-dependent kinase 2-like [Amblyomma americanum].
ACCESSION   XP_077503947
VERSION     XP_077503947.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647821.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..302
                     /product="cyclin-dependent kinase 2-like"
                     /calculated_mol_wt=34390
     Region          9..293
                     /region_name="STKc_CDK1_CdkB_like"
                     /note="Catalytic domain of Cyclin-Dependent protein Kinase
                     1-like Serine/Threonine Kinases and of Plant B-type
                     Cyclin-Dependent protein Kinase; cd07835"
                     /db_xref="CDD:270829"
     Site            order(15..18,23,36,38,55,69,85..88,91,93..94,134,136,
                     138..139,141,152,155,167,169..172,174,212..213)
                     /site_type="active"
                     /db_xref="CDD:270829"
     Site            order(15..18,23,36,38,69,85..88,91,94,138..139,141,152)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270829"
     Site            order(42,47..49,51,54..55,57..58,61..62,76,123,126..129,
                     157..159,161..162,166..167,279,283..285)
                     /site_type="other"
                     /note="CDK/cyclin interface [polypeptide binding]"
                     /db_xref="CDD:270829"
     Site            order(55,93,134,136,155,167,169..172,174,212..213)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270829"
     Site            151..174
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270829"
     CDS             1..302
                     /gene="LOC144114223"
                     /coded_by="XM_077647821.1:97..1005"
                     /db_xref="GeneID:144114223"
ORIGIN      
        1 mengfndkfq kigkigegty gvvykardka tgqyialkki rlaheaegvp staireiall
       61 kelrhpnivr lldvvphdkk lylvfeymse dlkkhmdkaa stktpltaql vksylwqllq
      121 giaychshri lhrdlkpqnl lidlegnikl adfglarafg mplraythev vtlwyrapei
      181 llgtrlyatp vdvwsigcif aemlllkplf qgdseidqlf rifrtlgtpd edswpgvtnl
      241 pdykptfprw epqslvnivt gldrdgedli mkllvadpea ripaktalsh ryfrdvtiqr
      301 pq
//
LOCUS       XP_077503948             388 aa            linear   INV 25-NOV-2025
DEFINITION  snRNA-activating protein complex subunit 3-like [Amblyomma
            americanum].
ACCESSION   XP_077503948
VERSION     XP_077503948.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647822.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..388
                     /product="snRNA-activating protein complex subunit 3-like"
                     /calculated_mol_wt=44564
     Region          178..381
                     /region_name="SNAPC3"
                     /note="snRNA-activating protein complex (SNAPc), subunit
                     3; pfam12251"
                     /db_xref="CDD:463506"
     CDS             1..388
                     /gene="LOC144114225"
                     /coded_by="XM_077647822.1:37..1203"
                     /db_xref="GeneID:144114225"
ORIGIN      
        1 mdavhepncr efisepvkva efkkqwtnpl easryyefdg mnigeairka mniseselkr
       61 ledefrpdkl ccglekldst evpevtdlnt ikelekklqk qqqdmtyryk easlvrrscp
      121 ltsvnlvneh shveppdter vpsgeviltv qvfkpikapv cftkvhrgcn dfpftilsev
      181 avlgsqtlle lrakiqcisd dvpigdfsen pdkpreptag evyksgfffi gdtfyndmsd
      241 pscrdysevi iewakkprrg lgpfkkaime dvtfeqleir lgypyvyvhq gscehllvfs
      301 dirmhhahds qhlldypfvv knfpvgkrvl cnlcrkstak wvtignrrvp ddpfffcavc
      361 frkfnytadn kkigsfqafp ykdwnaav
//
LOCUS       XP_077503949             388 aa            linear   INV 25-NOV-2025
DEFINITION  snRNA-activating protein complex subunit 3-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077503949
VERSION     XP_077503949.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647823.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..388
                     /product="snRNA-activating protein complex subunit 3-like
                     isoform X1"
                     /calculated_mol_wt=44564
     Region          178..381
                     /region_name="SNAPC3"
                     /note="snRNA-activating protein complex (SNAPc), subunit
                     3; pfam12251"
                     /db_xref="CDD:463506"
     CDS             1..388
                     /gene="LOC144114228"
                     /coded_by="XM_077647823.1:147..1313"
                     /db_xref="GeneID:144114228"
ORIGIN      
        1 mdavhepncr efisepvkva efkkqwtnpl easryyefdg mnigeairka mniseselkr
       61 ledefrpdkl ccglekldst evpevtdlnt ikelekklqk qqqdmtyryk easlvrrscp
      121 ltsvnlvneh shveppdter vpsgeviltv qvfkpikapv cftkvhrgcn dfpftilsev
      181 avlgsqtlle lrakiqcisd dvpigdfsen pdkpreptag evyksgfffi gdtfyndmsd
      241 pscrdysevi iewakkprrg lgpfkkaime dvtfeqleir lgypyvyvhq gscehllvfs
      301 dirmhhahds qhlldypfvv knfpvgkrvl cnlcrkstak wvtignrrvp ddpfffcavc
      361 frkfnytadn kkigsfqafp ykdwnaav
//
LOCUS       XP_077503950             337 aa            linear   INV 25-NOV-2025
DEFINITION  snRNA-activating protein complex subunit 3-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077503950
VERSION     XP_077503950.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647824.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..337
                     /product="snRNA-activating protein complex subunit 3-like
                     isoform X2"
                     /calculated_mol_wt=38852
     Region          127..330
                     /region_name="SNAPC3"
                     /note="snRNA-activating protein complex (SNAPc), subunit
                     3; pfam12251"
                     /db_xref="CDD:463506"
     CDS             1..337
                     /gene="LOC144114228"
                     /coded_by="XM_077647824.1:147..1160"
                     /db_xref="GeneID:144114228"
ORIGIN      
        1 mdavhepncr efisepvkva efkkqwtnpl easryyefdg mnigeairka mniseselkr
       61 ledefrpdkl ccglekldst evpevtdlnt ikelekklqk qqqdmtyryk easlvrrscp
      121 ltsvilseva vlgsqtllel rakiqcisdd vpigdfsenp dkpreptage vyksgfffig
      181 dtfyndmsdp scrdysevii ewakkprrgl gpfkkaimed vtfeqleirl gypyvyvhqg
      241 scehllvfsd irmhhahdsq hlldypfvvk nfpvgkrvlc nlcrkstakw vtignrrvpd
      301 dpfffcavcf rkfnytadnk kigsfqafpy kdwnaav
//
LOCUS       XP_077503951             388 aa            linear   INV 25-NOV-2025
DEFINITION  snRNA-activating protein complex subunit 3-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077503951
VERSION     XP_077503951.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647825.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..388
                     /product="snRNA-activating protein complex subunit 3-like
                     isoform X1"
                     /calculated_mol_wt=44564
     Region          178..381
                     /region_name="SNAPC3"
                     /note="snRNA-activating protein complex (SNAPc), subunit
                     3; pfam12251"
                     /db_xref="CDD:463506"
     CDS             1..388
                     /gene="LOC144114230"
                     /coded_by="XM_077647825.1:135..1301"
                     /db_xref="GeneID:144114230"
ORIGIN      
        1 mdavhepncr efisepvkva efkkqwtnpl easryyefdg mnigeairka mniseselkr
       61 ledefrpdkl ccglekldst evpevtdlnt ikelekklqk qqqdmtyryk easlvrrscp
      121 ltsvnlvneh shveppdter vpsgeviltv qvfkpikapv cftkvhrgcn dfpftilsev
      181 avlgsqtlle lrakiqcisd dvpigdfsen pdkpreptag evyksgfffi gdtfyndmsd
      241 pscrdysevi iewakkprrg lgpfkkaime dvtfeqleir lgypyvyvhq gscehllvfs
      301 dirmhhahds qhlldypfvv knfpvgkrvl cnlcrkstak wvtignrrvp ddpfffcavc
      361 frkfnytadn kkigsfqafp ykdwnaav
//
LOCUS       XP_077503953             388 aa            linear   INV 25-NOV-2025
DEFINITION  snRNA-activating protein complex subunit 3-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077503953
VERSION     XP_077503953.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647827.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..388
                     /product="snRNA-activating protein complex subunit 3-like
                     isoform X1"
                     /calculated_mol_wt=44564
     Region          178..381
                     /region_name="SNAPC3"
                     /note="snRNA-activating protein complex (SNAPc), subunit
                     3; pfam12251"
                     /db_xref="CDD:463506"
     CDS             1..388
                     /gene="LOC144114230"
                     /coded_by="XM_077647827.1:113..1279"
                     /db_xref="GeneID:144114230"
ORIGIN      
        1 mdavhepncr efisepvkva efkkqwtnpl easryyefdg mnigeairka mniseselkr
       61 ledefrpdkl ccglekldst evpevtdlnt ikelekklqk qqqdmtyryk easlvrrscp
      121 ltsvnlvneh shveppdter vpsgeviltv qvfkpikapv cftkvhrgcn dfpftilsev
      181 avlgsqtlle lrakiqcisd dvpigdfsen pdkpreptag evyksgfffi gdtfyndmsd
      241 pscrdysevi iewakkprrg lgpfkkaime dvtfeqleir lgypyvyvhq gscehllvfs
      301 dirmhhahds qhlldypfvv knfpvgkrvl cnlcrkstak wvtignrrvp ddpfffcavc
      361 frkfnytadn kkigsfqafp ykdwnaav
//
LOCUS       XP_077503954             337 aa            linear   INV 25-NOV-2025
DEFINITION  snRNA-activating protein complex subunit 3-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077503954
VERSION     XP_077503954.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647828.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..337
                     /product="snRNA-activating protein complex subunit 3-like
                     isoform X2"
                     /calculated_mol_wt=38852
     Region          127..330
                     /region_name="SNAPC3"
                     /note="snRNA-activating protein complex (SNAPc), subunit
                     3; pfam12251"
                     /db_xref="CDD:463506"
     CDS             1..337
                     /gene="LOC144114230"
                     /coded_by="XM_077647828.1:114..1127"
                     /db_xref="GeneID:144114230"
ORIGIN      
        1 mdavhepncr efisepvkva efkkqwtnpl easryyefdg mnigeairka mniseselkr
       61 ledefrpdkl ccglekldst evpevtdlnt ikelekklqk qqqdmtyryk easlvrrscp
      121 ltsvilseva vlgsqtllel rakiqcisdd vpigdfsenp dkpreptage vyksgfffig
      181 dtfyndmsdp scrdysevii ewakkprrgl gpfkkaimed vtfeqleirl gypyvyvhqg
      241 scehllvfsd irmhhahdsq hlldypfvvk nfpvgkrvlc nlcrkstakw vtignrrvpd
      301 dpfffcavcf rkfnytadnk kigsfqafpy kdwnaav
//
LOCUS       XP_077503955             317 aa            linear   INV 25-NOV-2025
DEFINITION  palmitoyltransferase ZDHHC3 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503955
VERSION     XP_077503955.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647829.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..317
                     /product="palmitoyltransferase ZDHHC3 isoform X1"
                     /calculated_mol_wt=35549
     Region          137..273
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; pfam01529"
                     /db_xref="CDD:396215"
     CDS             1..317
                     /gene="LOC144114231"
                     /coded_by="XM_077647829.1:122..1075"
                     /db_xref="GeneID:144114231"
ORIGIN      
        1 mamrldcplr rshrvvqagp vlpmavqgrv spatddrlkc cgrrfwfvrd icgvicavmt
       61 wllvlyaqyv vvgvillpsl htyygafhll lfellaflav fshvrtmvtd pgavprgtat
      121 reaveqlglr egrlvykcpk csclkperah hcsvcqrcir kmdhhcpwvn ncigennqkf
      181 fvlftlyiav ischafflav nhfvgcinse wstrgvshas tgcstgspav tvillillif
      241 eallfaiftl vmfasqlqai wndetgieql kkevarwqkr spwrsmravf grfslswfsp
      301 ftsagdsppl pgylyav
//
LOCUS       XP_077503956             308 aa            linear   INV 25-NOV-2025
DEFINITION  palmitoyltransferase ZDHHC3 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503956
VERSION     XP_077503956.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647830.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..308
                     /product="palmitoyltransferase ZDHHC3 isoform X2"
                     /calculated_mol_wt=34751
     Region          137..264
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; pfam01529"
                     /db_xref="CDD:396215"
     CDS             1..308
                     /gene="LOC144114231"
                     /coded_by="XM_077647830.1:155..1081"
                     /db_xref="GeneID:144114231"
ORIGIN      
        1 mamrldcplr rshrvvqagp vlpmavqgrv spatddrlkc cgrrfwfvrd icgvicavmt
       61 wllvlyaqyv vvgvillpsl htyygafhll lfellaflav fshvrtmvtd pgavprgtat
      121 reaveqlglr egrlvykcpk csclkperah hcsvcqrcir kmdhhcpwvn ncigennqkf
      181 fvlftlyiav ischafflav nhfvgcinse wsrcstgspa vtvillilli feallfaift
      241 lvmfasqlqa iwndetgieq lkkevarwqk rspwrsmrav fgrfslswfs pftsagdspp
      301 lpgylyav
//
LOCUS       XP_077503957             294 aa            linear   INV 25-NOV-2025
DEFINITION  palmitoyltransferase ZDHHC3 isoform X3 [Amblyomma americanum].
ACCESSION   XP_077503957
VERSION     XP_077503957.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647831.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..294
                     /product="palmitoyltransferase ZDHHC3 isoform X3"
                     /calculated_mol_wt=32964
     Region          114..250
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; pfam01529"
                     /db_xref="CDD:396215"
     CDS             1..294
                     /gene="LOC144114231"
                     /coded_by="XM_077647831.1:151..1035"
                     /db_xref="GeneID:144114231"
ORIGIN      
        1 mavqgrvspa tddrlkccgr rfwfvrdicg vicavmtwll vlyaqyvvvg villpslhty
       61 ygafhlllfe llaflavfsh vrtmvtdpga vprgtatrea veqlglregr lvykcpkcsc
      121 lkperahhcs vcqrcirkmd hhcpwvnnci gennqkffvl ftlyiavisc hafflavnhf
      181 vgcinsewst rgvshastgc stgspavtvi llillifeal lfaiftlvmf asqlqaiwnd
      241 etgieqlkke varwqkrspw rsmravfgrf slswfspfts agdspplpgy lyav
//
LOCUS       XP_077503958             144 aa            linear   INV 25-NOV-2025
DEFINITION  DNA-directed RNA polymerase II subunit RPB4-like [Amblyomma
            americanum].
ACCESSION   XP_077503958
VERSION     XP_077503958.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647832.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..144
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..144
                     /product="DNA-directed RNA polymerase II subunit
                     RPB4-like"
                     /calculated_mol_wt=16592
     Region          26..143
                     /region_name="RPOL4c"
                     /note="DNA-directed RNA-polymerase II subunit; smart00657"
                     /db_xref="CDD:128904"
     CDS             1..144
                     /gene="LOC144114232"
                     /coded_by="XM_077647832.1:208..642"
                     /db_xref="GeneID:144114232"
ORIGIN      
        1 manvpaaaap vldqveedas elqfpkefen aetllisevh mllehrkqqn esaeeeqels
       61 evfmktlgyc qrfsrfknre tisavrqlla qkklhrfela qlanlcpdnp eesralvpsl
      121 egrfeddqlr qilddiqtqr sfqy
//
LOCUS       XP_077503959             245 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114234 [Amblyomma americanum].
ACCESSION   XP_077503959
VERSION     XP_077503959.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647833.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..245
                     /product="uncharacterized protein LOC144114234"
                     /calculated_mol_wt=26797
     Region          16..>73
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..245
                     /gene="LOC144114234"
                     /coded_by="XM_077647833.1:1317..2054"
                     /db_xref="GeneID:144114234"
ORIGIN      
        1 maeaalpnrk rgpkyccvvg chnsaentke ldppvklyrf pgkwyekerl qawinavrrv
       61 rwqrrlnqai ssrqlsagse npsrstddea tmhkpgtsit dcrhqdihmm dlsdaplems
      121 aavastists dshpsmsvcg tgeshfiakr daasqpkdsv aqgkltills atngtdastq
      181 vvhtemsdkv asmdgnwkkn wisslsvskg krksaarfmw sdtasllppf epaaasevqa
      241 qycvk
//
LOCUS       XP_077503960             926 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase MARCHF6 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503960
VERSION     XP_077503960.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647834.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..926
                     /product="E3 ubiquitin-protein ligase MARCHF6 isoform X1"
                     /calculated_mol_wt=104041
     Region          9..>110
                     /region_name="SSM4"
                     /note="E3 ubiquitin-protein ligase DOA10
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG5183"
                     /db_xref="CDD:227510"
     Region          9..58
                     /region_name="RING_CH-C4HC3_MARCH6"
                     /note="RING-CH finger, H2 subclass (C4HC3-type), found in
                     membrane-associated RING-CH6 (MARCH6); cd16702"
                     /db_xref="CDD:438362"
     Region          <325..>468
                     /region_name="SSM4"
                     /note="E3 ubiquitin-protein ligase DOA10
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG5183"
                     /db_xref="CDD:227510"
     CDS             1..926
                     /gene="LOC144114236"
                     /coded_by="XM_077647834.1:67..2847"
                     /db_xref="GeneID:144114236"
ORIGIN      
        1 mddsqlqdic rvcrsegapd kplfhpcict gsikyihqdc lvqwlkysrk eycelcnhrf
       61 sfmpiyspdm pkrlpirdiv sgllsslgta irywlhytvv afawlgivpl tacriyrclf
      121 tgsvsslltl pldmlstenl lsdslygcci vtctlcafis lvwlreqvih gegpdwleqd
      181 pqqpeglaea epgeapgppl gpmphhaeep paqaddhvaq ddinwnpiew draaeeltwe
      241 rllgldgslv flehvfwvvs lntlfilvfa fcpyhigqfa imgfrlksyv avsqfegllt
      301 tlcgyvllgv vlvflhglaa lvklrrtqrl lglcyvvvkv allsvveigl fplvcgwwld
      361 vcslsmldat lsdressfql apgtfmflhw lvgmvyvfyf asfvlllrev lrpgllwflr
      421 nlndpdfnpi qemihlpilq hvrrflvslv ifgttvllmv wvpvrliqfl lpgflpyhvl
      481 issiayhfap qhhamflesp sqeislelil lqvvlptlle qghmkqwlka lvrawclava
      541 clldlrsyll gdapdtqqeq dgavgagqea edgsqlmapq nggdeevdde eeeeeeeeee
      601 eeeeeedddd eeagpapvvl gaahqallqg agpsgfqayq rprwfplrla allccialsl
      661 talsfamlti pvsmgrlvmm vlmpggrvhe fytaacglys cwlalrcltl lcswlprgwn
      721 tilsklhswm lisvksvvam slllglipll mgllfdlvvi iplrvplnat plfylwhdwa
      781 lgvlhtkiic avtlmgpnww lkrvidemyq dgvrqlnlrl vvldlvvpvv vvlglalavp
      841 yllaaslvpa fgssaetqnl vlrriypsll alcafmafmv fqvrqfcrly ehikndkylv
      901 grrlvnyehr rpassfatts qltass
//
LOCUS       XP_077503961             912 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase MARCHF6 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503961
VERSION     XP_077503961.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647835.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..912
                     /product="E3 ubiquitin-protein ligase MARCHF6 isoform X2"
                     /calculated_mol_wt=102376
     Region          9..>110
                     /region_name="SSM4"
                     /note="E3 ubiquitin-protein ligase DOA10
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG5183"
                     /db_xref="CDD:227510"
     Region          9..58
                     /region_name="RING_CH-C4HC3_MARCH6"
                     /note="RING-CH finger, H2 subclass (C4HC3-type), found in
                     membrane-associated RING-CH6 (MARCH6); cd16702"
                     /db_xref="CDD:438362"
     Region          <325..>504
                     /region_name="SSM4"
                     /note="E3 ubiquitin-protein ligase DOA10
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG5183"
                     /db_xref="CDD:227510"
     CDS             1..912
                     /gene="LOC144114236"
                     /coded_by="XM_077647835.1:67..2805"
                     /db_xref="GeneID:144114236"
ORIGIN      
        1 mddsqlqdic rvcrsegapd kplfhpcict gsikyihqdc lvqwlkysrk eycelcnhrf
       61 sfmpiyspdm pkrlpirdiv sgllsslgta irywlhytvv afawlgivpl tacriyrclf
      121 tgsvsslltl pldmlstenl lsdslygcci vtctlcafis lvwlreqvih gegpdwleqd
      181 pqqpeglaea epgeapgppl gpmphhaeep paqaddhvaq ddinwnpiew draaeeltwe
      241 rllgldgslv flehvfwvvs lntlfilvfa fcpyhigqfa imgfrlksyv avsqfegllt
      301 tlcgyvllgv vlvflhglaa lvklrrtqrl lglcyvvvkv allsvveigl fplvcgwwld
      361 vcslsmldat lsdressfql apgtfmflhw lvgmvyvfyf asfvlllrev lrpgllwflr
      421 nlndpdfnpi qemihlpilq hvrrflvslv ifgttvllmv wvpvrliqfl lpgflpyhvl
      481 issespsqei slelillqvv lptlleqghm kqwlkalvra wclavaclld lrsyllgdap
      541 dtqqeqdgav gagqeaedgs qlmapqnggd eevddeeeee eeeeeeeeee eeddddeeag
      601 papvvlgaah qallqgagps gfqayqrprw fplrlaallc cialsltals famltipvsm
      661 grlvmmvlmp ggrvhefyta acglyscwla lrcltllcsw lprgwntils klhswmlisv
      721 ksvvamslll glipllmgll fdlvviiplr vplnatplfy lwhdwalgvl htkiicavtl
      781 mgpnwwlkrv idemyqdgvr qlnlrlvvld lvvpvvvvlg lalavpylla aslvpafgss
      841 aetqnlvlrr iypsllalca fmafmvfqvr qfcrlyehik ndkylvgrrl vnyehrrpas
      901 sfattsqlta ss
//
LOCUS       XP_077503962             371 aa            linear   INV 25-NOV-2025
DEFINITION  ribonuclease P protein subunit p40-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503962
VERSION     XP_077503962.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647836.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..371
                     /product="ribonuclease P protein subunit p40-like isoform
                     X1"
                     /calculated_mol_wt=41203
     Region          77..320
                     /region_name="Ribonuc_P_40"
                     /note="Ribonuclease P 40kDa (Rpp40) subunit; pfam08584"
                     /db_xref="CDD:462526"
     CDS             1..371
                     /gene="LOC144114237"
                     /coded_by="XM_077647836.1:152..1267"
                     /db_xref="GeneID:144114237"
ORIGIN      
        1 mwpdfpclnw didiclsrie dsgerwkeai srcnfihavq lvlpgvswvp ekihtlmaaq
       61 eyqvvhnvpp kdlvawdlve pfvrqgklll lsvntsvshg ncvaitadge lhlsmqedvf
      121 rmsglegcrs ktgsckehcv fgstidmqkq cfrpgknnye rtlrslercg dlmaqdvavi
      181 wepppelasl srlssgsyfs hcghtvttfk psyrsatevc qdvpaalttm ewqeeallsv
      241 hewlgavvcg vdskcsragn dargafissy hcpepstqvs rlfvadwrgf ftptcaiell
      301 sllrqhmvqe glpffglvlh rkdlatlgel lvaagsaplp cvhrlkekth thctkavfla
      361 wnihqqsaai l
//
LOCUS       XP_077503963             361 aa            linear   INV 25-NOV-2025
DEFINITION  ribonuclease P protein subunit p40-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503963
VERSION     XP_077503963.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647837.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..361
                     /product="ribonuclease P protein subunit p40-like isoform
                     X2"
                     /calculated_mol_wt=40272
     Region          77..326
                     /region_name="Ribonuc_P_40"
                     /note="Ribonuclease P 40kDa (Rpp40) subunit; pfam08584"
                     /db_xref="CDD:462526"
     CDS             1..361
                     /gene="LOC144114237"
                     /coded_by="XM_077647837.1:152..1237"
                     /db_xref="GeneID:144114237"
ORIGIN      
        1 mwpdfpclnw didiclsrie dsgerwkeai srcnfihavq lvlpgvswvp ekihtlmaaq
       61 eyqvvhnvpp kdlvawdlve pfvrqgklll lsvntsvshg ncvaitadge lhlsmqedvf
      121 rmsglegcrs ktgsckehcv fgstidmqkq cfrpgknnye rtlrslercg dlmaqdvavi
      181 wepppelasl srlssgsyfs hcghtvttfk psyrsatevc qdvpaalttm ewqeeallsv
      241 hewlgavvcg vdskcsragn dargafissy hcpepstqvs rlfvadwrgf ftptcaiell
      301 sllrqhmvqe glpffglvlh rfdsdptris snsqnlllsh sselsiictq dgrfclfksp
      361 k
//
LOCUS       XP_077503964             355 aa            linear   INV 25-NOV-2025
DEFINITION  ribonuclease P protein subunit p40-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077503964
VERSION     XP_077503964.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647838.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..355
                     /product="ribonuclease P protein subunit p40-like isoform
                     X3"
                     /calculated_mol_wt=39208
     Region          61..304
                     /region_name="Ribonuc_P_40"
                     /note="Ribonuclease P 40kDa (Rpp40) subunit; pfam08584"
                     /db_xref="CDD:462526"
     CDS             1..355
                     /gene="LOC144114237"
                     /coded_by="XM_077647838.1:24..1091"
                     /db_xref="GeneID:144114237"
ORIGIN      
        1 mtsrrfsksn rrknggrfac tlvqlvlpgv swvpekihtl maaqeyqvvh nvppkdlvaw
       61 dlvepfvrqg kllllsvnts vshgncvait adgelhlsmq edvfrmsgle gcrsktgsck
      121 ehcvfgstid mqkqcfrpgk nnyertlrsl ercgdlmaqd vaviwepppe laslsrlssg
      181 syfshcghtv ttfkpsyrsa tevcqdvpaa lttmewqeea llsvhewlga vvcgvdskcs
      241 ragndargaf issyhcpeps tqvsrlfvad wrgfftptca iellsllrqh mvqeglpffg
      301 lvlhrkdlat lgellvaags aplpcvhrlk ekththctka vflawnihqq saail
//
LOCUS       XP_077503965             226 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114238 [Amblyomma americanum].
ACCESSION   XP_077503965
VERSION     XP_077503965.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647839.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..226
                     /product="uncharacterized protein LOC144114238"
                     /calculated_mol_wt=23433
     Region          <75..199
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..226
                     /gene="LOC144114238"
                     /coded_by="XM_077647839.1:211..891"
                     /db_xref="GeneID:144114238"
ORIGIN      
        1 mgssesdaaq qgmfnakgvt likeavnglv kppepdgsvl dtpkislsnm ksridaalmv
       61 sggspgnlap cssdadaspp gpsrrsgrtp geqslqptak kvkverpesq setrtsgdtp
      121 rslrsgrsyt sdspaapktp plptakttna svsppaankp satvkteape plpppkpakq
      181 atpkagssav rvkqeladpl pglwhitpki aeemksiafr hfhfsm
//
LOCUS       XP_077503966             143 aa            linear   INV 25-NOV-2025
DEFINITION  NTF2-related export protein 1 [Amblyomma americanum].
ACCESSION   XP_077503966
VERSION     XP_077503966.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647840.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..143
                     /product="NTF2-related export protein 1"
                     /calculated_mol_wt=15810
     Region          18..139
                     /region_name="NTF2_like"
                     /note="Nuclear transport factor 2 (NTF2-like) superfamily.
                     This family includes members of the NTF2 family,
                     Delta-5-3-ketosteroid isomerases, Scytalone Dehydratases,
                     and the beta subunit of Ring hydroxylating dioxygenases.
                     This family is a classic example of...; cl09109"
                     /db_xref="CDD:471850"
     CDS             1..143
                     /gene="Nxt1"
                     /coded_by="XM_077647840.1:1..432"
                     /db_xref="GeneID:144114239"
ORIGIN      
        1 maaakgaetr tqqqreddaa ttgevfaklf yetlekrrhl lgnlfldsas llwngnphcg
       61 kdaiskfyal lpscvaelsc vdaqafrdef vngqttvhvv taglvkfsgq swmpytesfv
      121 liaqdsvwki vmdtfrfqep alv
//
LOCUS       XP_077503967            1362 aa            linear   INV 25-NOV-2025
DEFINITION  trafficking protein particle complex subunit 8 homolog l(3)76BDm
            isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503967
VERSION     XP_077503967.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647841.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1362
                     /product="trafficking protein particle complex subunit 8
                     homolog l(3)76BDm isoform X1"
                     /calculated_mol_wt=148318
     Region          165..599
                     /region_name="TRAPPC-Trs85"
                     /note="ER-Golgi trafficking TRAPP I complex 85 kDa
                     subunit; pfam12739"
                     /db_xref="CDD:463688"
     CDS             1..1362
                     /gene="l(3)76BDm"
                     /coded_by="XM_077647841.1:1015..5103"
                     /db_xref="GeneID:144114240"
ORIGIN      
        1 mmnankrtae dfiqgvfapt vtvqcssdae dvcrrnnlsf aellqpfcqq vevsvrppnv
       61 lqgtiaaklh lnirdlnspv pqlaaakkll ndsvanvqpp laentasltk ikagsynllv
      121 ststpwfysy rdaffrvlpp lehdftrhfl acifvvsteh acpeeelekm aqghqtgqse
      181 ggqrwffpqc lkyylllhdm slgsqakaea sfqvlkknfg ttschllqmn sasandpsaa
      241 slpdvwspfl hldapdkqvi gslegpatlr esnssldesv fqqgeeavpl eqaakekggv
      301 hhpllssgds spptlepplv eekgwqrkpg srlgscltqs dqarlrvfvq efcsqalvpy
      361 veeqmrilnd qvtnkkgihr slmsmkklfa gsktnpqgph asvnsvvygq dapelqvrrl
      421 gdlaflfqly efayqayhsa krdfngdsaw lhlagaqema alavfmagvn aqrpfpvrym
      481 dsaidlylnt ckvphlatra allsaeclwq vgqasvaagq lirltsegsd lrsallleqa
      541 alcflratpt cprkfafhlv laghrfakaa hrrhalhays qalqvyqgrg wslaeghmqf
      601 tigrqsaqlk qlvrareafh rllaqrsaqs psqqllflre fllvlrtqag sgrvelpvpr
      661 vcegtirlvl gaegsppgep wleevaaraa hggalpltfr pqlsclssat dnsvrplavv
      721 fepitvemel hnplqialql tnmhllwtft plddsgvvsn dgdtvqdcdl dgvirtdils
      781 evllepeqtq kvqlkvrpqq rgqlqvggla yclglspaqr eaaaplgplp spvhgyqplt
      841 lpaktspalp lkgprpdwrl qptvvgampr ltvtfselps qllcgevwka tlklhnaegc
      901 palhslrlac aqpqlfclcs asdeedeayh eeplrpsppv laqarplawv qapavgacme
      961 ggqavvlpfw lrgaekpgrh vlrllfyyqg pqgqlshrvl whqisfttlp sltlqasarq
     1021 svslgiggsp dgallslqvs nacetegldg fdlsllqvtc vsrvwslvpl tshpkfgspv
     1081 lkpqetknlv lrvtpfsskl sqdnttvvsq lpltrvqves grspclefcs wcgqelslpg
     1141 vdahvpqgag pasdvgpasp lleatlaviw kaavdqdgsv rvvrgqhhva lrefytvyss
     1201 lpgheptdlk paipdyptig lvaptfqyvr calvhpvqvk hdfvtnrllv ipvsllvhnp
     1261 sfrpvtvtve pgnrapiapt spsttpdlla advggfswtt avrrhlpiks httaavalsa
     1321 vfskagtynm aalqvsvltd dgrlshellc pslmvvqqpq tl
//
LOCUS       XP_077503968            1361 aa            linear   INV 25-NOV-2025
DEFINITION  trafficking protein particle complex subunit 8 homolog l(3)76BDm
            isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503968
VERSION     XP_077503968.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647842.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1361
                     /product="trafficking protein particle complex subunit 8
                     homolog l(3)76BDm isoform X2"
                     /calculated_mol_wt=148189
     Region          165..598
                     /region_name="TRAPPC-Trs85"
                     /note="ER-Golgi trafficking TRAPP I complex 85 kDa
                     subunit; pfam12739"
                     /db_xref="CDD:463688"
     CDS             1..1361
                     /gene="l(3)76BDm"
                     /coded_by="XM_077647842.1:1002..5087"
                     /db_xref="GeneID:144114240"
ORIGIN      
        1 mmnankrtae dfiqgvfapt vtvqcssdae dvcrrnnlsf aellqpfcqq vevsvrppnv
       61 lqgtiaaklh lnirdlnspv pqlaaakkll ndsvanvqpp laentasltk ikagsynllv
      121 ststpwfysy rdaffrvlpp lehdftrhfl acifvvsteh acpeeelekm aqghqtgqse
      181 ggqrwffpqc lkyylllhdm slgsqakaea sfqvlkknfg ttschllqmn sasandpsaa
      241 slpdvwspfl hldapdkvig slegpatlre snssldesvf qqgeeavple qaakekggvh
      301 hpllssgdss pptlepplve ekgwqrkpgs rlgscltqsd qarlrvfvqe fcsqalvpyv
      361 eeqmrilndq vtnkkgihrs lmsmkklfag sktnpqgpha svnsvvygqd apelqvrrlg
      421 dlaflfqlye fayqayhsak rdfngdsawl hlagaqemaa lavfmagvna qrpfpvrymd
      481 saidlylntc kvphlatraa llsaeclwqv gqasvaagql irltsegsdl rsallleqaa
      541 lcflratptc prkfafhlvl aghrfakaah rrhalhaysq alqvyqgrgw slaeghmqft
      601 igrqsaqlkq lvrareafhr llaqrsaqsp sqqllflref llvlrtqags grvelpvprv
      661 cegtirlvlg aegsppgepw leevaaraah ggalpltfrp qlsclssatd nsvrplavvf
      721 epitvemelh nplqialqlt nmhllwtftp lddsgvvsnd gdtvqdcdld gvirtdilse
      781 vllepeqtqk vqlkvrpqqr gqlqvgglay clglspaqre aaaplgplps pvhgyqpltl
      841 paktspalpl kgprpdwrlq ptvvgamprl tvtfselpsq llcgevwkat lklhnaegcp
      901 alhslrlaca qpqlfclcsa sdeedeayhe eplrpsppvl aqarplawvq apavgacmeg
      961 gqavvlpfwl rgaekpgrhv lrllfyyqgp qgqlshrvlw hqisfttlps ltlqasarqs
     1021 vslgiggspd gallslqvsn acetegldgf dlsllqvtcv srvwslvplt shpkfgspvl
     1081 kpqetknlvl rvtpfsskls qdnttvvsql pltrvqvesg rspclefcsw cgqelslpgv
     1141 dahvpqgagp asdvgpaspl leatlaviwk aavdqdgsvr vvrgqhhval refytvyssl
     1201 pgheptdlkp aipdyptigl vaptfqyvrc alvhpvqvkh dfvtnrllvi pvsllvhnps
     1261 frpvtvtvep gnrapiapts psttpdllaa dvggfswtta vrrhlpiksh ttaavalsav
     1321 fskagtynma alqvsvltdd grlshellcp slmvvqqpqt l
//
LOCUS       XP_077503969             761 aa            linear   INV 25-NOV-2025
DEFINITION  nephrin-like [Amblyomma americanum].
ACCESSION   XP_077503969
VERSION     XP_077503969.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647843.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..761
                     /product="nephrin-like"
                     /calculated_mol_wt=83947
     Region          36..149
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          174..239
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          <280..349
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          290..294
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          318..322
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          332..337
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          378..452
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          381..385
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          395..399
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          417..421
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          431..436
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          445..448
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          559..639
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(559,619,634)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(635..636,638..639)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     CDS             1..761
                     /gene="LOC144114241"
                     /coded_by="XM_077647843.1:160..2445"
                     /db_xref="GeneID:144114241"
ORIGIN      
        1 mcstcwirig rllhctltll tlgvpslmgl qplepprkvt vvltdgasad lpcdlgprsd
       61 pavkvwwhrv ddpttplytl dpppgrrgnl mqarhsvaes lrgrahfsav rrpallsisn
      121 lryedhglyv cnveykhgar rsydvelhii vppgkplild enlsalngtv gpsnegmlli
      181 lnchvvggkp eprvtwrqgs thlhhvfhrt eqgvkkatlh vrslrrdhlg avftceasns
      241 nltrpasssv tltmnlkpvr avirgkqdfl saefraevec eaegslppav vswwldgvrl
      301 naslaqtrls aggnvttsll gfvprardng rqlrcvatnp rmpgheevad vwllqvhykp
      361 rvslawgnrv nrssivqgtd vyfecevdan prlieiswrf eghelasrla nfivsnqsla
      421 lrgvqsansg hyscvaanae gaaesnrvfl rvqhlpvcdp nlrtkytaar heeirihcsv
      481 ladpwnvsfh wslngssghr rdlqwalsst tdsrglvtsy lrytprnesd fgtlscsarn
      541 aiglmmrpci ihvvpigppm pphncnvsnq tmgalrvdce ggaepghsfl levhelqrrr
      601 lvanlssptp sfwvddvppg tkykllvysf ngrvrsepvl lsthtlanaa qgfsepgppw
      661 lvssnilvsf lvvaicllva ipfvviiaik ykaktpedre dkqvhqekhw iqlsqgvehr
      721 nepsifpeea fvtamtsfqa stsptsvgcs dgtvlyprsv c
//
LOCUS       XP_077503970             155 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114243 [Amblyomma americanum].
ACCESSION   XP_077503970
VERSION     XP_077503970.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647844.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..155
                     /product="uncharacterized protein LOC144114243"
                     /calculated_mol_wt=16272
     CDS             1..155
                     /gene="LOC144114243"
                     /coded_by="XM_077647844.1:196..663"
                     /db_xref="GeneID:144114243"
ORIGIN      
        1 mssstttvrq tttvtsgssp vvalsvntsf ltsiggiltl ielilgiivf slcyqyaggh
       61 sgiflllisf aywlvsfyiv fsaflsasgt llpntffyfl fhvfgiilya aggiavlaql
      121 nrfspsgtia agamgliaai chvvhvafiy kgpkl
//
LOCUS       XP_077503971             155 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114243 [Amblyomma americanum].
ACCESSION   XP_077503971
VERSION     XP_077503971.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647845.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..155
                     /product="uncharacterized protein LOC144114243"
                     /calculated_mol_wt=16272
     CDS             1..155
                     /gene="LOC144114243"
                     /coded_by="XM_077647845.1:155..622"
                     /db_xref="GeneID:144114243"
ORIGIN      
        1 mssstttvrq tttvtsgssp vvalsvntsf ltsiggiltl ielilgiivf slcyqyaggh
       61 sgiflllisf aywlvsfyiv fsaflsasgt llpntffyfl fhvfgiilya aggiavlaql
      121 nrfspsgtia agamgliaai chvvhvafiy kgpkl
//
LOCUS       XP_077503972             148 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114244 [Amblyomma americanum].
ACCESSION   XP_077503972
VERSION     XP_077503972.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647846.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="uncharacterized protein LOC144114244"
                     /calculated_mol_wt=15569
     CDS             1..148
                     /gene="LOC144114244"
                     /coded_by="XM_077647846.1:246..692"
                     /db_xref="GeneID:144114244"
ORIGIN      
        1 mlwlvvmfgl aaqglssass lprcsdavca qdphciyars rttcwdvtgs statscrccq
       61 ncitggfise dcarqaqacv ladcpkvkct ygsyvpdcdc cnnclpqaca naedrnwpnc
      121 aateclscsn gtvaftcprt ccpvclrg
//
LOCUS       XP_077503974             417 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114245 [Amblyomma americanum].
ACCESSION   XP_077503974
VERSION     XP_077503974.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647848.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..417
                     /product="uncharacterized protein LOC144114245"
                     /calculated_mol_wt=46501
     CDS             1..417
                     /gene="LOC144114245"
                     /coded_by="XM_077647848.1:152..1405"
                     /db_xref="GeneID:144114245"
ORIGIN      
        1 mglpkpmhhk tfdaitkkvh aaavsavvkn lekaqeatkn evggldaavm fdgtwqkrgh
       61 kshngvgtvv sletglcldf evlsnychrc srhkelpgee eiwqafhgpv ceknvdcssh
      121 ameaeaarrt wqrtlsyetp lrftkflsdg dskayvavcd aqvygllnie kedctnhvak
      181 rlgsalrqlk tplprgqklk esavtklqty yqiavtsnrd siqkmysaiw asyfhscssd
      241 gasshkfcpe gpeswckhrr aealcqpapn htplltkaqg kavlpiykrl tdenllarci
      301 qgktqnaavs lnsriwllcp ktkfasrtvv ematalavlw fneghasfer vleelgvlps
      361 hqllmltdsq nerrikrmsa cqtaearahr rsmakrarve dssrkdregv tysagqf
//
LOCUS       XP_077503975             209 aa            linear   INV 25-NOV-2025
DEFINITION  PRELI domain containing slowmo [Amblyomma americanum].
ACCESSION   XP_077503975
VERSION     XP_077503975.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647849.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..209
                     /product="PRELI domain containing slowmo"
                     /calculated_mol_wt=23334
     Region          15..163
                     /region_name="PRELI"
                     /note="PRELI-like family; pfam04707"
                     /db_xref="CDD:398400"
     CDS             1..209
                     /gene="slmo"
                     /coded_by="XM_077647849.1:181..810"
                     /db_xref="GeneID:144114246"
ORIGIN      
        1 mkiwtsehtf nhpwetvaqa awrkypnpmn pavvgidvvd rkvhngvlks hrlistawgl
       61 ptwaqrilga drtcyasehs vvdparrtmt mmsrnltfcn eisiveklty tehpqqkgst
      121 lmtqeaviti rgvplssyle dfvakaissn agkgrlamew vigrmsqevq eltsrtvksv
      181 dgiitsaakk smddlshlaa sephyhfkn
//
LOCUS       XP_077503976             943 aa            linear   INV 25-NOV-2025
DEFINITION  clotting factor C-like [Amblyomma americanum].
ACCESSION   XP_077503976
VERSION     XP_077503976.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647850.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..943
                     /product="clotting factor C-like"
                     /calculated_mol_wt=100243
     Region          105..130
                     /region_name="EGF_2"
                     /note="EGF-like domain; pfam07974"
                     /db_xref="CDD:400365"
     Region          122..>258
                     /region_name="PHA02817"
                     /note="EEV Host range protein; Provisional"
                     /db_xref="CDD:165167"
     Region          195..256
                     /region_name="CCP"
                     /note="Complement control protein (CCP) modules (aka short
                     consensus repeats SCRs or SUSHI repeats) have been
                     identified in several proteins of the complement system;
                     cd00033"
                     /db_xref="CDD:153056"
     Site            order(205,230)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     Region          264..357
                     /region_name="LCCL"
                     /note="LCCL domain; pfam03815"
                     /db_xref="CDD:427521"
     Region          496..554
                     /region_name="CCP"
                     /note="Domain abundant in complement control proteins;
                     SUSHI repeat; short complement-like repeat (SCR);
                     smart00032"
                     /db_xref="CDD:214478"
     Site            order(506,528)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     Region          622..669
                     /region_name="CCP"
                     /note="Complement control protein (CCP) modules (aka short
                     consensus repeats SCRs or SUSHI repeats) have been
                     identified in several proteins of the complement system;
                     cd00033"
                     /db_xref="CDD:153056"
     Site            order(623,642)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     Region          683..941
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cd00190"
                     /db_xref="CDD:238113"
     Site            683
                     /site_type="active"
                     /note="cleavage site [active]"
                     /db_xref="CDD:238113"
     Site            order(732,788,889)
                     /site_type="active"
                     /db_xref="CDD:238113"
     Site            order(883,913,915)
                     /site_type="other"
                     /note="substrate binding sites [chemical binding]"
                     /db_xref="CDD:238113"
     CDS             1..943
                     /gene="LOC144114247"
                     /coded_by="XM_077647850.1:443..3274"
                     /db_xref="GeneID:144114247"
ORIGIN      
        1 milsvvcigv lsallpsvhs vdikgfckne shdcpcgstg stvnvkvdgc syvrrfefrc
       61 rpcdsdsydk vcpnfancqt canglkacet cppgrygvwc tsvctcqngg icdkdtgqcr
      121 clpgfsgslc eirdgctppa hphgllatma panlpkiihy ncptgfkrvg patlncqndg
      181 swngvppyce rqvpcpplpd vargvaqvsg spsspreiqy ngttvtysca dgyemvgsks
      241 lqctqqgaws apppsclkvs emevtcstka neilgddsdt avrvhcppgc glvsgsvigs
      301 fqyhvlssvc raavhagrln naggavhvrd tgayadfaas tshgvssanf sspgasfkfv
      361 tvddsqwrip eegcparwrd sgsfcfysag ksrpqvaasn fcrnlgsqap airgdnatrt
      421 llseflaasg iaetwlsesr vqspflpvae ngdgaggacq alviansgqa evvtrpcsls
      481 lpvtcvalkt lklvqcedpg pikdgsalve rashgrfleg sriayfckel hylsgegtit
      541 ctangtwsaq kprcvkvitc gepevpshgt irvlppirgg dprrgpvrgh aragvpvrlq
      601 rplslsstay daadeskaae eanfvlppdh hrvgsraeya clpqyemags tvrrclssgq
      661 wsglpatcip vcgrsdsprs pliyngnasd vgqwpwqaal svlnpesngs adwvvncggs
      721 llsetwvlta ahcvtyessr iviprdilrv slgkhfrsds kddsyvqarq vaeihvnfdy
      781 dptsydndia lvqlaepvel tprvrpvclp tdrsarvhlq egklgvvtgw gltesgqyad
      841 vlseavlpvv qnekcqeaye lagvpltvse amfcaghana tsdacsgdsg gpmvflddav
      901 tterrwileg vvswgspsgc avanqyggft rvfaflpwir qfv
//
LOCUS       XP_077503977             215 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114248 [Amblyomma americanum].
ACCESSION   XP_077503977
VERSION     XP_077503977.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647851.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..215
                     /product="uncharacterized protein LOC144114248"
                     /calculated_mol_wt=17609
     CDS             1..215
                     /gene="LOC144114248"
                     /coded_by="XM_077647851.1:146..793"
                     /db_xref="GeneID:144114248"
ORIGIN      
        1 mkstglfcgl llvnavkagf lgggggglgg ggyggggygg ggfggggygg ggyggggygg
       61 ggyggggygg ggfgggglgg gghtstylvr tvskstgggg fggggygggl ggygggglgg
      121 yggggygggg yggggygggl ggfggggygg gagggakvii ikgggggglg gggyggglgg
      181 yggglggasy ggggyggggy ggggyggggh hggwh
//
LOCUS       XP_077503978             212 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114249 [Amblyomma americanum].
ACCESSION   XP_077503978
VERSION     XP_077503978.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647852.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..212
                     /product="uncharacterized protein LOC144114249"
                     /calculated_mol_wt=17596
     CDS             1..212
                     /gene="LOC144114249"
                     /coded_by="XM_077647852.1:92..730"
                     /db_xref="GeneID:144114249"
ORIGIN      
        1 mkstalfcgl llvnaakagf lgggggglgg ggyggggygg gglgglgggf ggyggggygg
       61 ggyggggygg ggyggggygg ggisqsylvr tvsktsgggg fggglggygg glggyggggy
      121 ggggyggysg ggfggggygg glggfggggy gggatggaak viiiksgggg glggglggfg
      181 glggagyggg gyggggfggg gyggghhggw hk
//
LOCUS       XP_077503979             211 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114250 [Amblyomma americanum].
ACCESSION   XP_077503979
VERSION     XP_077503979.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647853.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..211
                     /product="uncharacterized protein LOC144114250"
                     /calculated_mol_wt=17601
     CDS             1..211
                     /gene="LOC144114250"
                     /coded_by="XM_077647853.1:30..665"
                     /db_xref="GeneID:144114250"
ORIGIN      
        1 mkstalfcgl llvnaakagf lgggagglgg ggyggglggl gggfgghggg gyggggyggr
       61 gygggggygg ggyggggygg ggyggggtsy sylirtvskt sggggfgggl ggyegglggy
      121 ggggyggggr gglsgggygg glggfggggy gggagggapk viiiksgsgg glgaglgggf
      181 ggglggyggg glggggyggg gyggghhggw h
//
LOCUS       XP_077503980             540 aa            linear   INV 25-NOV-2025
DEFINITION  metabotropic glutamate receptor-like [Amblyomma americanum].
ACCESSION   XP_077503980
VERSION     XP_077503980.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647854.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..540
                     /product="metabotropic glutamate receptor-like"
                     /calculated_mol_wt=59557
     Region          20..472
                     /region_name="Periplasmic_Binding_Protein_type1"
                     /note="Type 1 periplasmic binding fold superfamily;
                     cl10011"
                     /db_xref="CDD:471960"
     Region          496..537
                     /region_name="NCD3G"
                     /note="Nine Cysteines Domain of family 3 GPCR; pfam07562"
                     /db_xref="CDD:462210"
     CDS             1..540
                     /gene="LOC144114251"
                     /coded_by="XM_077647854.1:158..1780"
                     /db_xref="GeneID:144114251"
ORIGIN      
        1 mtfkfillav maldsqatri dipgdimlgg lfpvhhrigr sacgpinger giqrleamlf
       61 aldrinqdps llngislgat ildtcssseh avrealhfvq ppadtrslkc vdgsfphlde
      121 nrqkitgvvg gsysevsqnv arllgifrvp qvspastass lgdktryeyf arivppdtlq
      181 ahvmadivhh fnwkyvslvt seglygdsgm tsflrdarsg nfcvavletv prlatestfd
      241 giirgllrrp narvvalftr gddargllaa akranatghl vwlasdgwgr qtqvveglee
      301 qaegaitvel dsriipefde ymknltvltn krnpwfreyw edvfqcnasd paqenstlas
      361 caeslrldes sgyrqsskiq fvidsvyafa haldeaskql cqgqnesyce alrqldggsf
      421 ymnyllnttf qdiagstvqf dqlgdglvpy riynyrrlsn sseydyvpvg rwepytlqln
      481 sekvrwsssd etgqapvsac sadcsvgyik vlspgnkccw lcsacgpvrt videytcsdn
//
LOCUS       XP_077503981             584 aa            linear   INV 25-NOV-2025
DEFINITION  snake venom 5'-nucleotidase isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503981
VERSION     XP_077503981.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647855.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..584
                     /product="snake venom 5'-nucleotidase isoform X1"
                     /calculated_mol_wt=65660
     Region          8..245
                     /region_name="MPP_CG11883_N"
                     /note="Drosophila melanogaster CG11883 and related
                     proteins, N-terminal metallophosphatase domain; cd07406"
                     /db_xref="CDD:277351"
     Site            order(15,17,52,84..85,187,210,212)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:277351"
     Site            order(15,17,52,84,187,210,212)
                     /site_type="other"
                     /note="putative metal binding site [ion binding]"
                     /db_xref="CDD:277351"
     Region          283..441
                     /region_name="5_nucleotid_C"
                     /note="5'-nucleotidase, C-terminal domain; pfam02872"
                     /db_xref="CDD:427027"
     CDS             1..584
                     /gene="LOC144114253"
                     /coded_by="XM_077647855.1:157..1911"
                     /db_xref="GeneID:144114253"
ORIGIN      
        1 mpeegevvtv lhfndcynve eqhgepigga trfctalksf ahlnplilfs gdvlapsims
       61 tftkgeqmvp vlncckvdca vygnhdfdfg vdnltefakq tkfpwlmsnv idnetgrlla
      121 dgeefqiler sgkkigligl veeewlatla tidpedveyk dfvtegrrla rllkqkkgvd
      181 yvialthmrt pndcrlaanv devdlilggh dhvyevkkvn gkhviksgtd frqfsritlt
      241 ftasgvvvdv aevnvtsdfv edselrdlls kykdvvqgkm eevighfavd ldgrfssirt
      301 setnlgnfic dvmlssthsd lailnsgtlr sdrihrkgpf tmrdlvtilp mmdslivlsa
      361 tgdqilqale ngvsqypkle grfpqvsgvs fafdpkkppg qridpqfvri gdepldrnqk
      421 yrlvtknyla qgkdgydvfk ecevllrede spelctavqn hfqavriltg vahprthhrq
      481 slvclsrrhs lvrtcedvtt kpplkrgfsl dasarrglfr qrqtsledve hetckmepkv
      541 enriviltee qvvkkfweds dshplrlvee vieeepstgs fekn
//
LOCUS       XP_077503982             584 aa            linear   INV 25-NOV-2025
DEFINITION  snake venom 5'-nucleotidase isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503982
VERSION     XP_077503982.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647856.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..584
                     /product="snake venom 5'-nucleotidase isoform X1"
                     /calculated_mol_wt=65660
     Region          8..245
                     /region_name="MPP_CG11883_N"
                     /note="Drosophila melanogaster CG11883 and related
                     proteins, N-terminal metallophosphatase domain; cd07406"
                     /db_xref="CDD:277351"
     Site            order(15,17,52,84..85,187,210,212)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:277351"
     Site            order(15,17,52,84,187,210,212)
                     /site_type="other"
                     /note="putative metal binding site [ion binding]"
                     /db_xref="CDD:277351"
     Region          283..441
                     /region_name="5_nucleotid_C"
                     /note="5'-nucleotidase, C-terminal domain; pfam02872"
                     /db_xref="CDD:427027"
     CDS             1..584
                     /gene="LOC144114253"
                     /coded_by="XM_077647856.1:161..1915"
                     /db_xref="GeneID:144114253"
ORIGIN      
        1 mpeegevvtv lhfndcynve eqhgepigga trfctalksf ahlnplilfs gdvlapsims
       61 tftkgeqmvp vlncckvdca vygnhdfdfg vdnltefakq tkfpwlmsnv idnetgrlla
      121 dgeefqiler sgkkigligl veeewlatla tidpedveyk dfvtegrrla rllkqkkgvd
      181 yvialthmrt pndcrlaanv devdlilggh dhvyevkkvn gkhviksgtd frqfsritlt
      241 ftasgvvvdv aevnvtsdfv edselrdlls kykdvvqgkm eevighfavd ldgrfssirt
      301 setnlgnfic dvmlssthsd lailnsgtlr sdrihrkgpf tmrdlvtilp mmdslivlsa
      361 tgdqilqale ngvsqypkle grfpqvsgvs fafdpkkppg qridpqfvri gdepldrnqk
      421 yrlvtknyla qgkdgydvfk ecevllrede spelctavqn hfqavriltg vahprthhrq
      481 slvclsrrhs lvrtcedvtt kpplkrgfsl dasarrglfr qrqtsledve hetckmepkv
      541 enriviltee qvvkkfweds dshplrlvee vieeepstgs fekn
//
LOCUS       XP_077503983             584 aa            linear   INV 25-NOV-2025
DEFINITION  snake venom 5'-nucleotidase isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503983
VERSION     XP_077503983.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647857.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..584
                     /product="snake venom 5'-nucleotidase isoform X1"
                     /calculated_mol_wt=65660
     Region          8..245
                     /region_name="MPP_CG11883_N"
                     /note="Drosophila melanogaster CG11883 and related
                     proteins, N-terminal metallophosphatase domain; cd07406"
                     /db_xref="CDD:277351"
     Site            order(15,17,52,84..85,187,210,212)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:277351"
     Site            order(15,17,52,84,187,210,212)
                     /site_type="other"
                     /note="putative metal binding site [ion binding]"
                     /db_xref="CDD:277351"
     Region          283..441
                     /region_name="5_nucleotid_C"
                     /note="5'-nucleotidase, C-terminal domain; pfam02872"
                     /db_xref="CDD:427027"
     CDS             1..584
                     /gene="LOC144114253"
                     /coded_by="XM_077647857.1:178..1932"
                     /db_xref="GeneID:144114253"
ORIGIN      
        1 mpeegevvtv lhfndcynve eqhgepigga trfctalksf ahlnplilfs gdvlapsims
       61 tftkgeqmvp vlncckvdca vygnhdfdfg vdnltefakq tkfpwlmsnv idnetgrlla
      121 dgeefqiler sgkkigligl veeewlatla tidpedveyk dfvtegrrla rllkqkkgvd
      181 yvialthmrt pndcrlaanv devdlilggh dhvyevkkvn gkhviksgtd frqfsritlt
      241 ftasgvvvdv aevnvtsdfv edselrdlls kykdvvqgkm eevighfavd ldgrfssirt
      301 setnlgnfic dvmlssthsd lailnsgtlr sdrihrkgpf tmrdlvtilp mmdslivlsa
      361 tgdqilqale ngvsqypkle grfpqvsgvs fafdpkkppg qridpqfvri gdepldrnqk
      421 yrlvtknyla qgkdgydvfk ecevllrede spelctavqn hfqavriltg vahprthhrq
      481 slvclsrrhs lvrtcedvtt kpplkrgfsl dasarrglfr qrqtsledve hetckmepkv
      541 enriviltee qvvkkfweds dshplrlvee vieeepstgs fekn
//
LOCUS       XP_077503984             583 aa            linear   INV 25-NOV-2025
DEFINITION  snake venom 5'-nucleotidase isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503984
VERSION     XP_077503984.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647858.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..583
                     /product="snake venom 5'-nucleotidase isoform X2"
                     /calculated_mol_wt=65532
     Region          8..245
                     /region_name="MPP_CG11883_N"
                     /note="Drosophila melanogaster CG11883 and related
                     proteins, N-terminal metallophosphatase domain; cd07406"
                     /db_xref="CDD:277351"
     Site            order(15,17,52,84..85,187,210,212)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:277351"
     Site            order(15,17,52,84,187,210,212)
                     /site_type="other"
                     /note="putative metal binding site [ion binding]"
                     /db_xref="CDD:277351"
     Region          283..441
                     /region_name="5_nucleotid_C"
                     /note="5'-nucleotidase, C-terminal domain; pfam02872"
                     /db_xref="CDD:427027"
     CDS             1..583
                     /gene="LOC144114253"
                     /coded_by="XM_077647858.1:157..1908"
                     /db_xref="GeneID:144114253"
ORIGIN      
        1 mpeegevvtv lhfndcynve eqhgepigga trfctalksf ahlnplilfs gdvlapsims
       61 tftkgeqmvp vlncckvdca vygnhdfdfg vdnltefakq tkfpwlmsnv idnetgrlla
      121 dgeefqiler sgkkigligl veeewlatla tidpedveyk dfvtegrrla rllkqkkgvd
      181 yvialthmrt pndcrlaanv devdlilggh dhvyevkkvn gkhviksgtd frqfsritlt
      241 ftasgvvvdv aevnvtsdfv edselrdlls kykdvvqgkm eevighfavd ldgrfssirt
      301 setnlgnfic dvmlssthsd lailnsgtlr sdrihrkgpf tmrdlvtilp mmdslivlsa
      361 tgdqilqale ngvsqypkle grfpqvsgvs fafdpkkppg qridpqfvri gdepldrnqk
      421 yrlvtknyla qgkdgydvfk ecevllrede spelctavqn hfqavriltg vahprthhrq
      481 slvclsrrhs lvrtcedvtt kpplkrgfsl dasarrglfr qrqtsledve hetckmepkv
      541 enriviltee vvkkfwedsd shplrlveev ieeepstgsf ekn
//
LOCUS       XP_077503985             563 aa            linear   INV 25-NOV-2025
DEFINITION  snake venom 5'-nucleotidase isoform X3 [Amblyomma americanum].
ACCESSION   XP_077503985
VERSION     XP_077503985.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647859.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..563
                     /product="snake venom 5'-nucleotidase isoform X3"
                     /calculated_mol_wt=63159
     Region          8..245
                     /region_name="MPP_CG11883_N"
                     /note="Drosophila melanogaster CG11883 and related
                     proteins, N-terminal metallophosphatase domain; cd07406"
                     /db_xref="CDD:277351"
     Site            order(15,17,52,84..85,187,210,212)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:277351"
     Site            order(15,17,52,84,187,210,212)
                     /site_type="other"
                     /note="putative metal binding site [ion binding]"
                     /db_xref="CDD:277351"
     Region          283..441
                     /region_name="5_nucleotid_C"
                     /note="5'-nucleotidase, C-terminal domain; pfam02872"
                     /db_xref="CDD:427027"
     CDS             1..563
                     /gene="LOC144114253"
                     /coded_by="XM_077647859.1:157..1848"
                     /db_xref="GeneID:144114253"
ORIGIN      
        1 mpeegevvtv lhfndcynve eqhgepigga trfctalksf ahlnplilfs gdvlapsims
       61 tftkgeqmvp vlncckvdca vygnhdfdfg vdnltefakq tkfpwlmsnv idnetgrlla
      121 dgeefqiler sgkkigligl veeewlatla tidpedveyk dfvtegrrla rllkqkkgvd
      181 yvialthmrt pndcrlaanv devdlilggh dhvyevkkvn gkhviksgtd frqfsritlt
      241 ftasgvvvdv aevnvtsdfv edselrdlls kykdvvqgkm eevighfavd ldgrfssirt
      301 setnlgnfic dvmlssthsd lailnsgtlr sdrihrkgpf tmrdlvtilp mmdslivlsa
      361 tgdqilqale ngvsqypkle grfpqvsgvs fafdpkkppg qridpqfvri gdepldrnqk
      421 yrlvtknyla qgkdgydvfk ecevllrede spelctavqn hfqavriltg vahprthhrq
      481 slvclsrrhs lvrtcedvtt kpplkrgfsl dasarrglfr qrqtsledve hetckmepkv
      541 enriviltee vkwckpatfa qpl
//
LOCUS       XP_077503986             305 aa            linear   INV 25-NOV-2025
DEFINITION  syntaxin 16 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077503986
VERSION     XP_077503986.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647860.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..305
                     /product="syntaxin 16 isoform X1"
                     /calculated_mol_wt=35071
     Region          49..300
                     /region_name="COG5325"
                     /note="t-SNARE complex subunit, syntaxin [Intracellular
                     trafficking and secretion]"
                     /db_xref="CDD:227635"
     CDS             1..305
                     /gene="Syx16"
                     /coded_by="XM_077647860.1:150..1067"
                     /db_xref="GeneID:144114254"
ORIGIN      
        1 matrslteaf vlmrnnalqs rhifgdhvhe dtvalvgrdv eigigvykdd aepprwidal
       61 eevnyqmmki rdklkelsal hdrhlnrptf dessleedei eqtthqvtql fshcqqllsv
      121 iqqgvrhgsn pkethlaqnv vrsvasslqa lstsfrssqt tyckrlqsre ehsnkffhvp
      181 fyvedtqlsp drfggdhqle medqlfledn temvqvrere innilrsite lntifkdias
      241 mvaeqgtvld ridynldtvq trvqqglqql qkadtyqkkn hkmmcilvma astiiliill
      301 fafkl
//
LOCUS       XP_077503987             244 aa            linear   INV 25-NOV-2025
DEFINITION  syntaxin 16 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077503987
VERSION     XP_077503987.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647861.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..244
                     /product="syntaxin 16 isoform X2"
                     /calculated_mol_wt=28214
     Region          9..239
                     /region_name="COG5325"
                     /note="t-SNARE complex subunit, syntaxin [Intracellular
                     trafficking and secretion]"
                     /db_xref="CDD:227635"
     Region          153..211
                     /region_name="SNARE_syntaxin16"
                     /note="SNARE motif of syntaxin 16; cd15845"
                     /db_xref="CDD:277198"
     Site            order(154,156..157,159..164,166..168,170..171,173..175,
                     177..178,180..182,184..185,187..192,194..199,201..206,
                     208..210)
                     /site_type="other"
                     /note="heterotetramer interface [polypeptide binding]"
                     /db_xref="CDD:277198"
     Site            184
                     /site_type="other"
                     /note="zero layer"
                     /db_xref="CDD:277198"
     CDS             1..244
                     /gene="Syx16"
                     /coded_by="XM_077647861.1:314..1048"
                     /db_xref="GeneID:144114254"
ORIGIN      
        1 mvnyqmmkir dklkelsalh drhlnrptfd essleedeie qtthqvtqlf shcqqllsvi
       61 qqgvrhgsnp kethlaqnvv rsvasslqal stsfrssqtt yckrlqsree hsnkffhvpf
      121 yvedtqlspd rfggdhqlem edqlflednt emvqvrerei nnilrsitel ntifkdiasm
      181 vaeqgtvldr idynldtvqt rvqqglqqlq kadtyqkknh kmmcilvmaa stiiliillf
      241 afkl
//
LOCUS       XP_077503988             156 aa            linear   INV 25-NOV-2025
DEFINITION  sys1 golgi trafficking protein [Amblyomma americanum].
ACCESSION   XP_077503988
VERSION     XP_077503988.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647862.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..156
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..156
                     /product="sys1 golgi trafficking protein"
                     /calculated_mol_wt=17868
     Region          5..148
                     /region_name="SYS1"
                     /note="Integral membrane protein S linking to the trans
                     Golgi network; pfam09801"
                     /db_xref="CDD:370705"
     CDS             1..156
                     /gene="Sys1"
                     /coded_by="XM_077647862.1:286..756"
                     /db_xref="GeneID:144114255"
ORIGIN      
        1 maghfrysiw dpfliisqmi tlqcmyyvgl gfwicfldiv tgqvlsldql fayeavhikd
       61 mrgrcvigsy iinslfasll lwyfvqrtkq cldftctvhl fhligcwiyn safphtlsww
      121 llntacvtvt cvcgeflcir temkaiplsl spkadl
//
LOCUS       XP_077503989             966 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114256 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503989
VERSION     XP_077503989.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647863.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..966
                     /product="uncharacterized protein LOC144114256 isoform X1"
                     /calculated_mol_wt=105144
     Region          531..778
                     /region_name="GT8_Glycogenin"
                     /note="Glycogenin belongs the GT 8 family and initiates
                     the biosynthesis of glycogen; cd02537"
                     /db_xref="CDD:133018"
     Site            order(609..610,613,627,629..631,652,660..662,690..691,
                     717..718,738,740..741,744)
                     /site_type="active"
                     /note="substrate binding site [active]"
                     /db_xref="CDD:133018"
     Site            order(629,631,738)
                     /site_type="active"
                     /note="Manganese binding site [active]"
                     /db_xref="CDD:133018"
     Site            order(651..652,691,708,710..711,715,719)
                     /site_type="active"
                     /note="dimer interface [active]"
                     /db_xref="CDD:133018"
     Region          <810..907
                     /region_name="PHA03264"
                     /note="envelope glycoprotein D; Provisional"
                     /db_xref="CDD:223029"
     CDS             1..966
                     /gene="LOC144114256"
                     /coded_by="XM_077647863.1:173..3073"
                     /db_xref="GeneID:144114256"
ORIGIN      
        1 mssaqrepln tefsestefd rclppykein qadlpaslvi eddlhsltdv tasrpqcddl
       61 sctaqglpea vsarstirnn edkatpstvs ftyastdpks tdglelesfs fegsvsngpv
      121 pndmakspaq ashtaeptyp yptfpnvpdl qadisraiia sksnayrqig slqngsmgec
      181 kdgkkccnrh sqlasddfde yyikmldaqk kqpvlsqepp lctscsqsmm qyvsaiqtli
      241 qqltvdiivk gkdppdcswc areklaafqn swpssflkst sttvseglnq nstqqpcssl
      301 hkeqahvals rpqaqqwpgs sfrapvphsa hspnrgrnql lysaldvqnl pgngvcqgeg
      361 papfqslhlk ssvpagqape iaepvlsfnt rplntafiss ageqqnmpkq velspcspkl
      421 pegqgaaspk rslstvvcep sevvaqslkg degiinphyt esgdlqgsvk rpselqlynl
      481 gsslqdkpad savsegqhgg arrkqslnsd wmtdlvqpli pepavpsvrd qayvtmannd
      541 lsaivclvlg nslwlsktsr slvvlvtdgv shafrhllsc vfnvvlsvrs lgtqgttkla
      601 lleqpdigvs ftklhawrlt qfskcvfmda gmlvvqncde lferdelsav pdigwpdcfn
      661 sglfvyvpsm etfwdlisfa erqgsfdggd qgllntyfrn wssdinrrlp fiynlmanvc
      721 ytykpafkqf gqnvkvvqfl ggykpwnvkf hpptgtlspa advhptyvqf vqfwvqifvk
      781 rvlplfssni qaktneqryt caldilqhfp sflvaepmvc lpapslrahp npilylppgp
      841 rvapeppsag aslpssaatc pepatseaap eehaeppdvk ddsiplthse pspqkveaip
      901 nhlpvavddn cnfllqptdp aiavddfsgm laweqgrady mgehcsdnim akldeligpg
      961 pgvqak
//
LOCUS       XP_077503990             843 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114256 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503990
VERSION     XP_077503990.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647864.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..843
                     /product="uncharacterized protein LOC144114256 isoform X2"
                     /calculated_mol_wt=91870
     Region          408..655
                     /region_name="GT8_Glycogenin"
                     /note="Glycogenin belongs the GT 8 family and initiates
                     the biosynthesis of glycogen; cd02537"
                     /db_xref="CDD:133018"
     Site            order(486..487,490,504,506..508,529,537..539,567..568,
                     594..595,615,617..618,621)
                     /site_type="active"
                     /note="substrate binding site [active]"
                     /db_xref="CDD:133018"
     Site            order(506,508,615)
                     /site_type="active"
                     /note="Manganese binding site [active]"
                     /db_xref="CDD:133018"
     Site            order(528..529,568,585,587..588,592,596)
                     /site_type="active"
                     /note="dimer interface [active]"
                     /db_xref="CDD:133018"
     Region          <687..784
                     /region_name="PHA03264"
                     /note="envelope glycoprotein D; Provisional"
                     /db_xref="CDD:223029"
     CDS             1..843
                     /gene="LOC144114256"
                     /coded_by="XM_077647864.1:428..2959"
                     /db_xref="GeneID:144114256"
ORIGIN      
        1 makspaqash taeptypypt fpnvpdlqad israiiasks nayrqigslq ngsmgeckdg
       61 kkccnrhsql asddfdeyyi kmldaqkkqp vlsqepplct scsqsmmqyv saiqtliqql
      121 tvdiivkgkd ppdcswcare klaafqnswp ssflkststt vseglnqnst qqpcsslhke
      181 qahvalsrpq aqqwpgssfr apvphsahsp nrgrnqllys aldvqnlpgn gvcqgegpap
      241 fqslhlkssv pagqapeiae pvlsfntrpl ntafissage qqnmpkqvel spcspklpeg
      301 qgaaspkrsl stvvcepsev vaqslkgdeg iinphytesg dlqgsvkrps elqlynlgss
      361 lqdkpadsav segqhggarr kqslnsdwmt dlvqplipep avpsvrdqay vtmanndlsa
      421 ivclvlgnsl wlsktsrslv vlvtdgvsha frhllscvfn vvlsvrslgt qgttklalle
      481 qpdigvsftk lhawrltqfs kcvfmdagml vvqncdelfe rdelsavpdi gwpdcfnsgl
      541 fvyvpsmetf wdlisfaerq gsfdggdqgl lntyfrnwss dinrrlpfiy nlmanvcyty
      601 kpafkqfgqn vkvvqflggy kpwnvkfhpp tgtlspaadv hptyvqfvqf wvqifvkrvl
      661 plfssniqak tneqrytcal dilqhfpsfl vaepmvclpa pslrahpnpi lylppgprva
      721 peppsagasl pssaatcpep atseaapeeh aeppdvkdds iplthsepsp qkveaipnhl
      781 pvavddncnf llqptdpaia vddfsgmlaw eqgradymge hcsdnimakl deligpgpgv
      841 qak
//
LOCUS       XP_077503991             564 aa            linear   INV 25-NOV-2025
DEFINITION  beta-catenin-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077503991
VERSION     XP_077503991.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647865.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (GKSP01006159.1) annotated
            using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 73 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..564
                     /product="beta-catenin-like protein 1"
                     /calculated_mol_wt=64485
     Region          83..535
                     /region_name="CTNNBL"
                     /note="Catenin-beta-like, Arm-motif containing nuclear;
                     pfam08216"
                     /db_xref="CDD:462397"
     Region          138..171
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          186..216
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     CDS             1..564
                     /gene="LOC144114257"
                     /coded_by="XM_077647865.1:182..1876"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 73 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144114257"
ORIGIN      
        1 mdvgellnyk pakepkrgle ddeetadtrp rkrlqhnalt ggamrvsrfa plreepeptp
       61 seietadipq pdtdidildd tnlkklilnf ekkvlknqel rikypdlpek fmdsevelnd
      121 ilqemhvvat qpelypilve lnavqsilgl lshentdisi avvdllqelt dvdslhesqe
      181 gadmlidali dgqivaqlvq nldrldesik eeaegvhntl aivenltefr pdlsvqaaqq
      241 gllawilkrl kvkmpfeank lycseilsil lqnheenkkl lgeidgidtl lqqlsyfkrh
      301 dpsssdetem menlfgalcs slmcspnrer flkgeglqlm nlmlrekkms rsgalkvldf
      361 atsnvegtdn cnkfvdilgl rtvfplfmqt prkykkkvas peeheehvcs viasmlknck
      421 ptqrqrllnk ftendhekvd rlmelhfkyl ekvhaidnvi erekenpklq deddqmdqed
      481 kfylrrldag lftlqlvdyi mldicssgpp sikqrvlqil nlrggsikti rnimreyagn
      541 lgdakdeslk eveqqrilql vdrf
//
LOCUS       XP_077503992             897 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114258 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503992
VERSION     XP_077503992.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647866.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..897
                     /product="uncharacterized protein LOC144114258 isoform X1"
                     /calculated_mol_wt=97995
     Region          214..235
                     /region_name="zf-CCCH_4"
                     /note="CCCH-type zinc finger; pfam18044"
                     /db_xref="CDD:465626"
     CDS             1..897
                     /gene="LOC144114258"
                     /coded_by="XM_077647866.1:495..3188"
                     /db_xref="GeneID:144114258"
ORIGIN      
        1 mtsveelqkp avgssdasdg dgsaggslag pespeepnka leegnlvaer kseeaavqep
       61 qatavvpshs prgcsegvie seeegavhsp epapqlhltk dvsedaleaa pavpvakkcl
      121 nsrsmepleg eldfeddapp espsstspln kvpnkdsktk ednleegeae ddgevsdvsg
      181 telsvqdddl eegelpegtg gtgatrkaaq vprpicrffn kgqctwgssc rfihpgindr
      241 gnynmfatvp kplhhhmppp iggppavmrg gapppeflgp pmpplpppse emmppvesaw
      301 erglrhakel mkrasrrkeq epdfeekrln lvpshhdrtp lqsevdkend fyggsgvpps
      361 ramsppyvrp pmppppphhl arggrrsdgr rddfygfeep dplehserea reywrggrye
      421 nfevrwsnrq efdhrdrrek drrvgrsydr eksgyshgvr pdrphgggpe reprwreerf
      481 seappppmar trpdewrdpw rrsktpkgrr srsrgrnrrs fshcssrsfs srssrssysy
      541 srssssrssy ssydsrsrsr spapaappgr prgnqppprg ppmygppsqa pgkqgsaasg
      601 gtsggtgggr spvldrrkqy npspppvttp aspprksrrk apsspprkqk ksrsgsfsas
      661 ssrsrsrhtk lskgetkrgf mitssssrtp srsrsrsysr spslnsvssa ssspkrrrlq
      721 apstaaamla arnpsppkkg segakkrmek dpipkgvskl slqsqataal gpgvmmpsyr
      781 dplpppldla gfeeetaqrq areaalrqqg isqkpqiklt likktlplpl akkeepsakn
      841 ngdkaakkrp asppklvssp spppsggrks ttlrreellk qlkavedaia rkrakfs
//
LOCUS       XP_077503993             897 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114258 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503993
VERSION     XP_077503993.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647867.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..897
                     /product="uncharacterized protein LOC144114258 isoform X1"
                     /calculated_mol_wt=97995
     Region          214..235
                     /region_name="zf-CCCH_4"
                     /note="CCCH-type zinc finger; pfam18044"
                     /db_xref="CDD:465626"
     CDS             1..897
                     /gene="LOC144114258"
                     /coded_by="XM_077647867.1:208..2901"
                     /db_xref="GeneID:144114258"
ORIGIN      
        1 mtsveelqkp avgssdasdg dgsaggslag pespeepnka leegnlvaer kseeaavqep
       61 qatavvpshs prgcsegvie seeegavhsp epapqlhltk dvsedaleaa pavpvakkcl
      121 nsrsmepleg eldfeddapp espsstspln kvpnkdsktk ednleegeae ddgevsdvsg
      181 telsvqdddl eegelpegtg gtgatrkaaq vprpicrffn kgqctwgssc rfihpgindr
      241 gnynmfatvp kplhhhmppp iggppavmrg gapppeflgp pmpplpppse emmppvesaw
      301 erglrhakel mkrasrrkeq epdfeekrln lvpshhdrtp lqsevdkend fyggsgvpps
      361 ramsppyvrp pmppppphhl arggrrsdgr rddfygfeep dplehserea reywrggrye
      421 nfevrwsnrq efdhrdrrek drrvgrsydr eksgyshgvr pdrphgggpe reprwreerf
      481 seappppmar trpdewrdpw rrsktpkgrr srsrgrnrrs fshcssrsfs srssrssysy
      541 srssssrssy ssydsrsrsr spapaappgr prgnqppprg ppmygppsqa pgkqgsaasg
      601 gtsggtgggr spvldrrkqy npspppvttp aspprksrrk apsspprkqk ksrsgsfsas
      661 ssrsrsrhtk lskgetkrgf mitssssrtp srsrsrsysr spslnsvssa ssspkrrrlq
      721 apstaaamla arnpsppkkg segakkrmek dpipkgvskl slqsqataal gpgvmmpsyr
      781 dplpppldla gfeeetaqrq areaalrqqg isqkpqiklt likktlplpl akkeepsakn
      841 ngdkaakkrp asppklvssp spppsggrks ttlrreellk qlkavedaia rkrakfs
//
LOCUS       XP_077503994             897 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114258 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503994
VERSION     XP_077503994.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647868.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..897
                     /product="uncharacterized protein LOC144114258 isoform X1"
                     /calculated_mol_wt=97995
     Region          214..235
                     /region_name="zf-CCCH_4"
                     /note="CCCH-type zinc finger; pfam18044"
                     /db_xref="CDD:465626"
     CDS             1..897
                     /gene="LOC144114258"
                     /coded_by="XM_077647868.1:344..3037"
                     /db_xref="GeneID:144114258"
ORIGIN      
        1 mtsveelqkp avgssdasdg dgsaggslag pespeepnka leegnlvaer kseeaavqep
       61 qatavvpshs prgcsegvie seeegavhsp epapqlhltk dvsedaleaa pavpvakkcl
      121 nsrsmepleg eldfeddapp espsstspln kvpnkdsktk ednleegeae ddgevsdvsg
      181 telsvqdddl eegelpegtg gtgatrkaaq vprpicrffn kgqctwgssc rfihpgindr
      241 gnynmfatvp kplhhhmppp iggppavmrg gapppeflgp pmpplpppse emmppvesaw
      301 erglrhakel mkrasrrkeq epdfeekrln lvpshhdrtp lqsevdkend fyggsgvpps
      361 ramsppyvrp pmppppphhl arggrrsdgr rddfygfeep dplehserea reywrggrye
      421 nfevrwsnrq efdhrdrrek drrvgrsydr eksgyshgvr pdrphgggpe reprwreerf
      481 seappppmar trpdewrdpw rrsktpkgrr srsrgrnrrs fshcssrsfs srssrssysy
      541 srssssrssy ssydsrsrsr spapaappgr prgnqppprg ppmygppsqa pgkqgsaasg
      601 gtsggtgggr spvldrrkqy npspppvttp aspprksrrk apsspprkqk ksrsgsfsas
      661 ssrsrsrhtk lskgetkrgf mitssssrtp srsrsrsysr spslnsvssa ssspkrrrlq
      721 apstaaamla arnpsppkkg segakkrmek dpipkgvskl slqsqataal gpgvmmpsyr
      781 dplpppldla gfeeetaqrq areaalrqqg isqkpqiklt likktlplpl akkeepsakn
      841 ngdkaakkrp asppklvssp spppsggrks ttlrreellk qlkavedaia rkrakfs
//
LOCUS       XP_077503995             897 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114258 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503995
VERSION     XP_077503995.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647869.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..897
                     /product="uncharacterized protein LOC144114258 isoform X1"
                     /calculated_mol_wt=97995
     Region          214..235
                     /region_name="zf-CCCH_4"
                     /note="CCCH-type zinc finger; pfam18044"
                     /db_xref="CDD:465626"
     CDS             1..897
                     /gene="LOC144114258"
                     /coded_by="XM_077647869.1:1848..4541"
                     /db_xref="GeneID:144114258"
ORIGIN      
        1 mtsveelqkp avgssdasdg dgsaggslag pespeepnka leegnlvaer kseeaavqep
       61 qatavvpshs prgcsegvie seeegavhsp epapqlhltk dvsedaleaa pavpvakkcl
      121 nsrsmepleg eldfeddapp espsstspln kvpnkdsktk ednleegeae ddgevsdvsg
      181 telsvqdddl eegelpegtg gtgatrkaaq vprpicrffn kgqctwgssc rfihpgindr
      241 gnynmfatvp kplhhhmppp iggppavmrg gapppeflgp pmpplpppse emmppvesaw
      301 erglrhakel mkrasrrkeq epdfeekrln lvpshhdrtp lqsevdkend fyggsgvpps
      361 ramsppyvrp pmppppphhl arggrrsdgr rddfygfeep dplehserea reywrggrye
      421 nfevrwsnrq efdhrdrrek drrvgrsydr eksgyshgvr pdrphgggpe reprwreerf
      481 seappppmar trpdewrdpw rrsktpkgrr srsrgrnrrs fshcssrsfs srssrssysy
      541 srssssrssy ssydsrsrsr spapaappgr prgnqppprg ppmygppsqa pgkqgsaasg
      601 gtsggtgggr spvldrrkqy npspppvttp aspprksrrk apsspprkqk ksrsgsfsas
      661 ssrsrsrhtk lskgetkrgf mitssssrtp srsrsrsysr spslnsvssa ssspkrrrlq
      721 apstaaamla arnpsppkkg segakkrmek dpipkgvskl slqsqataal gpgvmmpsyr
      781 dplpppldla gfeeetaqrq areaalrqqg isqkpqiklt likktlplpl akkeepsakn
      841 ngdkaakkrp asppklvssp spppsggrks ttlrreellk qlkavedaia rkrakfs
//
LOCUS       XP_077503996             897 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114258 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503996
VERSION     XP_077503996.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647870.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..897
                     /product="uncharacterized protein LOC144114258 isoform X1"
                     /calculated_mol_wt=97995
     Region          214..235
                     /region_name="zf-CCCH_4"
                     /note="CCCH-type zinc finger; pfam18044"
                     /db_xref="CDD:465626"
     CDS             1..897
                     /gene="LOC144114258"
                     /coded_by="XM_077647870.1:4934..7627"
                     /db_xref="GeneID:144114258"
ORIGIN      
        1 mtsveelqkp avgssdasdg dgsaggslag pespeepnka leegnlvaer kseeaavqep
       61 qatavvpshs prgcsegvie seeegavhsp epapqlhltk dvsedaleaa pavpvakkcl
      121 nsrsmepleg eldfeddapp espsstspln kvpnkdsktk ednleegeae ddgevsdvsg
      181 telsvqdddl eegelpegtg gtgatrkaaq vprpicrffn kgqctwgssc rfihpgindr
      241 gnynmfatvp kplhhhmppp iggppavmrg gapppeflgp pmpplpppse emmppvesaw
      301 erglrhakel mkrasrrkeq epdfeekrln lvpshhdrtp lqsevdkend fyggsgvpps
      361 ramsppyvrp pmppppphhl arggrrsdgr rddfygfeep dplehserea reywrggrye
      421 nfevrwsnrq efdhrdrrek drrvgrsydr eksgyshgvr pdrphgggpe reprwreerf
      481 seappppmar trpdewrdpw rrsktpkgrr srsrgrnrrs fshcssrsfs srssrssysy
      541 srssssrssy ssydsrsrsr spapaappgr prgnqppprg ppmygppsqa pgkqgsaasg
      601 gtsggtgggr spvldrrkqy npspppvttp aspprksrrk apsspprkqk ksrsgsfsas
      661 ssrsrsrhtk lskgetkrgf mitssssrtp srsrsrsysr spslnsvssa ssspkrrrlq
      721 apstaaamla arnpsppkkg segakkrmek dpipkgvskl slqsqataal gpgvmmpsyr
      781 dplpppldla gfeeetaqrq areaalrqqg isqkpqiklt likktlplpl akkeepsakn
      841 ngdkaakkrp asppklvssp spppsggrks ttlrreellk qlkavedaia rkrakfs
//
LOCUS       XP_077503997             897 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114258 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077503997
VERSION     XP_077503997.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647871.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..897
                     /product="uncharacterized protein LOC144114258 isoform X1"
                     /calculated_mol_wt=97995
     Region          214..235
                     /region_name="zf-CCCH_4"
                     /note="CCCH-type zinc finger; pfam18044"
                     /db_xref="CDD:465626"
     CDS             1..897
                     /gene="LOC144114258"
                     /coded_by="XM_077647871.1:1929..4622"
                     /db_xref="GeneID:144114258"
ORIGIN      
        1 mtsveelqkp avgssdasdg dgsaggslag pespeepnka leegnlvaer kseeaavqep
       61 qatavvpshs prgcsegvie seeegavhsp epapqlhltk dvsedaleaa pavpvakkcl
      121 nsrsmepleg eldfeddapp espsstspln kvpnkdsktk ednleegeae ddgevsdvsg
      181 telsvqdddl eegelpegtg gtgatrkaaq vprpicrffn kgqctwgssc rfihpgindr
      241 gnynmfatvp kplhhhmppp iggppavmrg gapppeflgp pmpplpppse emmppvesaw
      301 erglrhakel mkrasrrkeq epdfeekrln lvpshhdrtp lqsevdkend fyggsgvpps
      361 ramsppyvrp pmppppphhl arggrrsdgr rddfygfeep dplehserea reywrggrye
      421 nfevrwsnrq efdhrdrrek drrvgrsydr eksgyshgvr pdrphgggpe reprwreerf
      481 seappppmar trpdewrdpw rrsktpkgrr srsrgrnrrs fshcssrsfs srssrssysy
      541 srssssrssy ssydsrsrsr spapaappgr prgnqppprg ppmygppsqa pgkqgsaasg
      601 gtsggtgggr spvldrrkqy npspppvttp aspprksrrk apsspprkqk ksrsgsfsas
      661 ssrsrsrhtk lskgetkrgf mitssssrtp srsrsrsysr spslnsvssa ssspkrrrlq
      721 apstaaamla arnpsppkkg segakkrmek dpipkgvskl slqsqataal gpgvmmpsyr
      781 dplpppldla gfeeetaqrq areaalrqqg isqkpqiklt likktlplpl akkeepsakn
      841 ngdkaakkrp asppklvssp spppsggrks ttlrreellk qlkavedaia rkrakfs
//
LOCUS       XP_077503999             881 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114258 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077503999
VERSION     XP_077503999.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647873.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..881
                     /product="uncharacterized protein LOC144114258 isoform X2"
                     /calculated_mol_wt=96179
     Region          214..235
                     /region_name="zf-CCCH_4"
                     /note="CCCH-type zinc finger; pfam18044"
                     /db_xref="CDD:465626"
     CDS             1..881
                     /gene="LOC144114258"
                     /coded_by="XM_077647873.1:495..3140"
                     /db_xref="GeneID:144114258"
ORIGIN      
        1 mtsveelqkp avgssdasdg dgsaggslag pespeepnka leegnlvaer kseeaavqep
       61 qatavvpshs prgcsegvie seeegavhsp epapqlhltk dvsedaleaa pavpvakkcl
      121 nsrsmepleg eldfeddapp espsstspln kvpnkdsktk ednleegeae ddgevsdvsg
      181 telsvqdddl eegelpegtg gtgatrkaaq vprpicrffn kgqctwgssc rfihpgindr
      241 gnynmfatvp kplhhhmppp iggppavmrg gapppeflgp pmpplpppse emmppvesaw
      301 erglrhakel mkrasrrkeq epdfeekrln lvpshhdrtp lqsevdkend fyggsgvpps
      361 ramsppyvrp pmppppphhl arggrrsdgr rddfygfeep dplehserea reywrggrye
      421 nfevrwsnrq efdhrdrrek drrvgrsydr eksgyshgvr pdrphgggpe reprwreerf
      481 seappppmar trpdewrdpw rrsktpkgrr srsrgrnrrs fshcssrsfs srssrssysy
      541 srssssrssy ssydsrsrsr spapaappgr prgnqppprg ppmygppsqa pgkqgsaasg
      601 gtsggtgggr spvldrrkqy npspppvttp aspprksrrk apsspprkqk ksrsgsfsas
      661 ssrsrsrsss srtpsrsrsr sysrspslns vssassspkr rrlqapstaa amlaarnpsp
      721 pkkgsegakk rmekdpipkg vsklslqsqa taalgpgvmm psyrdplppp ldlagfeeet
      781 aqrqareaal rqqgisqkpq ikltlikktl plplakkeep saknngdkaa kkrpasppkl
      841 vsspspppsg grksttlrre ellkqlkave daiarkrakf s
//
LOCUS       XP_077504000             881 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114258 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504000
VERSION     XP_077504000.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647874.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..881
                     /product="uncharacterized protein LOC144114258 isoform X2"
                     /calculated_mol_wt=96179
     Region          214..235
                     /region_name="zf-CCCH_4"
                     /note="CCCH-type zinc finger; pfam18044"
                     /db_xref="CDD:465626"
     CDS             1..881
                     /gene="LOC144114258"
                     /coded_by="XM_077647874.1:208..2853"
                     /db_xref="GeneID:144114258"
ORIGIN      
        1 mtsveelqkp avgssdasdg dgsaggslag pespeepnka leegnlvaer kseeaavqep
       61 qatavvpshs prgcsegvie seeegavhsp epapqlhltk dvsedaleaa pavpvakkcl
      121 nsrsmepleg eldfeddapp espsstspln kvpnkdsktk ednleegeae ddgevsdvsg
      181 telsvqdddl eegelpegtg gtgatrkaaq vprpicrffn kgqctwgssc rfihpgindr
      241 gnynmfatvp kplhhhmppp iggppavmrg gapppeflgp pmpplpppse emmppvesaw
      301 erglrhakel mkrasrrkeq epdfeekrln lvpshhdrtp lqsevdkend fyggsgvpps
      361 ramsppyvrp pmppppphhl arggrrsdgr rddfygfeep dplehserea reywrggrye
      421 nfevrwsnrq efdhrdrrek drrvgrsydr eksgyshgvr pdrphgggpe reprwreerf
      481 seappppmar trpdewrdpw rrsktpkgrr srsrgrnrrs fshcssrsfs srssrssysy
      541 srssssrssy ssydsrsrsr spapaappgr prgnqppprg ppmygppsqa pgkqgsaasg
      601 gtsggtgggr spvldrrkqy npspppvttp aspprksrrk apsspprkqk ksrsgsfsas
      661 ssrsrsrsss srtpsrsrsr sysrspslns vssassspkr rrlqapstaa amlaarnpsp
      721 pkkgsegakk rmekdpipkg vsklslqsqa taalgpgvmm psyrdplppp ldlagfeeet
      781 aqrqareaal rqqgisqkpq ikltlikktl plplakkeep saknngdkaa kkrpasppkl
      841 vsspspppsg grksttlrre ellkqlkave daiarkrakf s
//
LOCUS       XP_077504001             881 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114258 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504001
VERSION     XP_077504001.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647875.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..881
                     /product="uncharacterized protein LOC144114258 isoform X2"
                     /calculated_mol_wt=96179
     Region          214..235
                     /region_name="zf-CCCH_4"
                     /note="CCCH-type zinc finger; pfam18044"
                     /db_xref="CDD:465626"
     CDS             1..881
                     /gene="LOC144114258"
                     /coded_by="XM_077647875.1:643..3288"
                     /db_xref="GeneID:144114258"
ORIGIN      
        1 mtsveelqkp avgssdasdg dgsaggslag pespeepnka leegnlvaer kseeaavqep
       61 qatavvpshs prgcsegvie seeegavhsp epapqlhltk dvsedaleaa pavpvakkcl
      121 nsrsmepleg eldfeddapp espsstspln kvpnkdsktk ednleegeae ddgevsdvsg
      181 telsvqdddl eegelpegtg gtgatrkaaq vprpicrffn kgqctwgssc rfihpgindr
      241 gnynmfatvp kplhhhmppp iggppavmrg gapppeflgp pmpplpppse emmppvesaw
      301 erglrhakel mkrasrrkeq epdfeekrln lvpshhdrtp lqsevdkend fyggsgvpps
      361 ramsppyvrp pmppppphhl arggrrsdgr rddfygfeep dplehserea reywrggrye
      421 nfevrwsnrq efdhrdrrek drrvgrsydr eksgyshgvr pdrphgggpe reprwreerf
      481 seappppmar trpdewrdpw rrsktpkgrr srsrgrnrrs fshcssrsfs srssrssysy
      541 srssssrssy ssydsrsrsr spapaappgr prgnqppprg ppmygppsqa pgkqgsaasg
      601 gtsggtgggr spvldrrkqy npspppvttp aspprksrrk apsspprkqk ksrsgsfsas
      661 ssrsrsrsss srtpsrsrsr sysrspslns vssassspkr rrlqapstaa amlaarnpsp
      721 pkkgsegakk rmekdpipkg vsklslqsqa taalgpgvmm psyrdplppp ldlagfeeet
      781 aqrqareaal rqqgisqkpq ikltlikktl plplakkeep saknngdkaa kkrpasppkl
      841 vsspspppsg grksttlrre ellkqlkave daiarkrakf s
//
LOCUS       XP_077504002             881 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114258 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504002
VERSION     XP_077504002.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647876.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..881
                     /product="uncharacterized protein LOC144114258 isoform X2"
                     /calculated_mol_wt=96179
     Region          214..235
                     /region_name="zf-CCCH_4"
                     /note="CCCH-type zinc finger; pfam18044"
                     /db_xref="CDD:465626"
     CDS             1..881
                     /gene="LOC144114258"
                     /coded_by="XM_077647876.1:344..2989"
                     /db_xref="GeneID:144114258"
ORIGIN      
        1 mtsveelqkp avgssdasdg dgsaggslag pespeepnka leegnlvaer kseeaavqep
       61 qatavvpshs prgcsegvie seeegavhsp epapqlhltk dvsedaleaa pavpvakkcl
      121 nsrsmepleg eldfeddapp espsstspln kvpnkdsktk ednleegeae ddgevsdvsg
      181 telsvqdddl eegelpegtg gtgatrkaaq vprpicrffn kgqctwgssc rfihpgindr
      241 gnynmfatvp kplhhhmppp iggppavmrg gapppeflgp pmpplpppse emmppvesaw
      301 erglrhakel mkrasrrkeq epdfeekrln lvpshhdrtp lqsevdkend fyggsgvpps
      361 ramsppyvrp pmppppphhl arggrrsdgr rddfygfeep dplehserea reywrggrye
      421 nfevrwsnrq efdhrdrrek drrvgrsydr eksgyshgvr pdrphgggpe reprwreerf
      481 seappppmar trpdewrdpw rrsktpkgrr srsrgrnrrs fshcssrsfs srssrssysy
      541 srssssrssy ssydsrsrsr spapaappgr prgnqppprg ppmygppsqa pgkqgsaasg
      601 gtsggtgggr spvldrrkqy npspppvttp aspprksrrk apsspprkqk ksrsgsfsas
      661 ssrsrsrsss srtpsrsrsr sysrspslns vssassspkr rrlqapstaa amlaarnpsp
      721 pkkgsegakk rmekdpipkg vsklslqsqa taalgpgvmm psyrdplppp ldlagfeeet
      781 aqrqareaal rqqgisqkpq ikltlikktl plplakkeep saknngdkaa kkrpasppkl
      841 vsspspppsg grksttlrre ellkqlkave daiarkrakf s
//
LOCUS       XP_077504003             217 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome c oxidase subunit 4 isoform 1, mitochondrial-like
            isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504003
VERSION     XP_077504003.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647877.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="cytochrome c oxidase subunit 4 isoform 1,
                     mitochondrial-like isoform X1"
                     /calculated_mol_wt=24610
     Region          76..211
                     /region_name="Cyt_c_Oxidase_IV"
                     /note="Cytochrome c oxidase subunit IV. Cytochrome c
                     oxidase (CcO), the terminal oxidase in the respiratory
                     chains of eukaryotes and most bacteria, is a multi-chain
                     transmembrane protein located in the inner membrane of
                     mitochondria and the cell membrane of...; cd00922"
                     /db_xref="CDD:238462"
     Site            order(80,162,173)
                     /site_type="other"
                     /note="Subunit IV/VIIIb interface [polypeptide binding]"
                     /db_xref="CDD:238462"
     Site            84
                     /site_type="other"
                     /note="Subunit IV/Vb interface [polypeptide binding]"
                     /db_xref="CDD:238462"
     Site            order(90,92,94..97,115,117,119,127,131..132,134..135,
                     137..140,143)
                     /site_type="other"
                     /note="Subunit IV/Va interface [polypeptide binding]"
                     /db_xref="CDD:238462"
     Site            order(91..93,148,155,159,163,167,176,178,181..182)
                     /site_type="other"
                     /note="Subunit IV/I interface [polypeptide binding]"
                     /db_xref="CDD:238462"
     Site            order(92,193..194,197,201,203..205)
                     /site_type="other"
                     /note="Subunit IV/II interface [polypeptide binding]"
                     /db_xref="CDD:238462"
     Site            order(92,144,146,148..149)
                     /site_type="other"
                     /note="putative ATP/ADP binding site [chemical binding]"
                     /db_xref="CDD:238462"
     Site            order(150..151,157..158,160..162,164..165,182,185,
                     188..189,192,196,205,209)
                     /site_type="other"
                     /note="Subunit IV/VIIb interface [polypeptide binding]"
                     /db_xref="CDD:238462"
     Site            order(198,203)
                     /site_type="other"
                     /note="Subunit IV/VIc interface [polypeptide binding]"
                     /db_xref="CDD:238462"
     CDS             1..217
                     /gene="LOC144114261"
                     /coded_by="XM_077647877.1:15..668"
                     /db_xref="GeneID:144114261"
ORIGIN      
        1 mllapsaill shadtfhalf hassasaasa rsyshcysma srllqisera vklapvvara
       61 qpaaayhgra iigkrevvgf glngeyayhd tpdfpmpair ykeptpeiek lrekekgdwk
      121 nlsleekkal yrysffqtfa emnasrnewk pivgnvflml gvslwiwifl kkfvygplps
      181 tttlearqaq lqrmidlrvn pvegisskwd yennrwk
//
LOCUS       XP_077504004             179 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome c oxidase subunit 4 isoform 1, mitochondrial-like
            isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504004
VERSION     XP_077504004.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647878.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..179
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..179
                     /product="cytochrome c oxidase subunit 4 isoform 1,
                     mitochondrial-like isoform X2"
                     /calculated_mol_wt=20635
     Region          38..173
                     /region_name="Cyt_c_Oxidase_IV"
                     /note="Cytochrome c oxidase subunit IV. Cytochrome c
                     oxidase (CcO), the terminal oxidase in the respiratory
                     chains of eukaryotes and most bacteria, is a multi-chain
                     transmembrane protein located in the inner membrane of
                     mitochondria and the cell membrane of...; cd00922"
                     /db_xref="CDD:238462"
     Site            order(42,124,135)
                     /site_type="other"
                     /note="Subunit IV/VIIIb interface [polypeptide binding]"
                     /db_xref="CDD:238462"
     Site            46
                     /site_type="other"
                     /note="Subunit IV/Vb interface [polypeptide binding]"
                     /db_xref="CDD:238462"
     Site            order(52,54,56..59,77,79,81,89,93..94,96..97,99..102,105)
                     /site_type="other"
                     /note="Subunit IV/Va interface [polypeptide binding]"
                     /db_xref="CDD:238462"
     Site            order(53..55,110,117,121,125,129,138,140,143..144)
                     /site_type="other"
                     /note="Subunit IV/I interface [polypeptide binding]"
                     /db_xref="CDD:238462"
     Site            order(54,155..156,159,163,165..167)
                     /site_type="other"
                     /note="Subunit IV/II interface [polypeptide binding]"
                     /db_xref="CDD:238462"
     Site            order(54,106,108,110..111)
                     /site_type="other"
                     /note="putative ATP/ADP binding site [chemical binding]"
                     /db_xref="CDD:238462"
     Site            order(112..113,119..120,122..124,126..127,144,147,
                     150..151,154,158,167,171)
                     /site_type="other"
                     /note="Subunit IV/VIIb interface [polypeptide binding]"
                     /db_xref="CDD:238462"
     Site            order(160,165)
                     /site_type="other"
                     /note="Subunit IV/VIc interface [polypeptide binding]"
                     /db_xref="CDD:238462"
     CDS             1..179
                     /gene="LOC144114261"
                     /coded_by="XM_077647878.1:27..566"
                     /db_xref="GeneID:144114261"
ORIGIN      
        1 masrllqise ravklapvva raqpaaayhg raiigkrevv gfglngeyay hdtpdfpmpa
       61 irykeptpei eklrekekgd wknlsleekk alyrysffqt faemnasrne wkpivgnvfl
      121 mlgvslwiwi flkkfvygpl pstttlearq aqlqrmidlr vnpvegissk wdyennrwk
//
LOCUS       XP_077504005             538 aa            linear   INV 25-NOV-2025
DEFINITION  lysophosphatidylcholine acyltransferase [Amblyomma americanum].
ACCESSION   XP_077504005
VERSION     XP_077504005.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647879.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..538
                     /product="lysophosphatidylcholine acyltransferase"
                     /calculated_mol_wt=60574
     Region          137..345
                     /region_name="LPLAT_LPCAT1-like"
                     /note="Lysophospholipid Acyltransferases (LPLATs) of
                     Glycerophospholipid Biosynthesis: LPCAT1-like; cd07991"
                     /db_xref="CDD:153253"
     Site            order(166,169,171,185..188,241..243)
                     /site_type="active"
                     /note="putative acyl-acceptor binding pocket [active]"
                     /db_xref="CDD:153253"
     CDS             1..538
                     /gene="LPCAT"
                     /coded_by="XM_077647879.1:221..1837"
                     /db_xref="GeneID:144114262"
ORIGIN      
        1 mneadspaak rpifdasrfp lgdakilpdy tqlkapdeid nmtrrlidgs yspsgdlapn
       61 pfhyrihltr adkikvalms vivvpvrivl iiffllltwl gcylgqlgls hkdrtekplt
      121 gfrrdylkpf lafflrltfe cggltysfkg klasqkeapv lvvgphssll dgvvvlllgg
      181 ltpvakagse nvpifgtiln ftqpvyvkrs dpnsrqntvr eitrratske pwsqviifpe
      241 gtctnrtcli sfklgafvpg vpiqpvliry pnelntliwt wdgpsafetl wltlcqlrth
      301 meieylpvyv pserekkdpk lyaenvrclm akalgiplss ysyddlkhvd medsargktv
      361 vcsrlkhlis rnriqfrdlg dvilkvkaai knressrvsf celvsilgvp sdeisrdvfe
      421 vldidrtkqm dlrallaalw lvhraerhrt eqafkafcdg sngilledfa lfiwillelp
      481 rsaamsmfqk issaslgrvy fedfkefist tkwadvlpap sdssdprded ksvpsked
//
LOCUS       XP_077504006             824 aa            linear   INV 25-NOV-2025
DEFINITION  Na(+)/H(+) exchanger protein 2-like [Amblyomma americanum].
ACCESSION   XP_077504006
VERSION     XP_077504006.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647880.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..824
                     /product="Na(+)/H(+) exchanger protein 2-like"
                     /calculated_mol_wt=91407
     Region          1..430
                     /region_name="Na_H_Exchanger"
                     /note="Sodium/hydrogen exchanger family; cl01133"
                     /db_xref="CDD:470090"
     CDS             1..824
                     /gene="LOC144114263"
                     /coded_by="XM_077647880.1:140..2614"
                     /db_xref="GeneID:144114263"
ORIGIN      
        1 mlppivldag yympnrsffd nlgsimtyav lgtvwnalti glclwavgls glygtpvpll
       61 dallfssiis avdpvavlav feeihvndvl yilvfgesll ndavtvvlyr mfegytdmgt
      121 eniiptdyvy gvasffvval ggtavgilwg lvaafvsrft hhvraieplf vfilgylsyl
      181 saelfhlsgi laltfcgltm knyveenisq kshttlkygm kmlancseti ifmflgvstv
      241 ndihewntwf viltivfitv frsvgvvllt wpinrfrvha lnkvdqfima ygglrgavaf
      301 alvlvvneev iptkkmlvtt tiaviyftvf vqgmtigplv rllnvprsvk gqmsmnerlh
      361 trlmdhlmag ledvtgqfmg nykirdkfky ynnrflrpml lkdhsirepk iietysklnl
      421 adamnllknn nslvpvmnge hgvslaslfr sytqtnlnkg aasasgdtlr dldlpvdrsa
      481 avlnfdmnel qyspsskdha daqlhhilsd smfkpsrkvk rlyrdrvdvp edqpafhhht
      541 rmqirtflgg drrrmrkarn grlprhegnd agrltcrhah eprkipngdk pqlrfslpmn
      601 gdcslnidch qltassvaas kpeassslqq ggddegivfv arprdespak gsprsysppp
      661 ppealtetet tlpwkredvp daagvrarcg sgdsgvrqre fpswidnrey iayqsptsti
      721 lgrldrhkrs tpdvfevfql kssgapaapm eqvteeeqdg gspevvspse ptqaaasera
      781 qgaeegpsst astepvapas rweetvidle sddsesyssr hvrl
//
LOCUS       XP_077504007             481 aa            linear   INV 25-NOV-2025
DEFINITION  (Lyso)-N-acylphosphatidylethanolamine lipase-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077504007
VERSION     XP_077504007.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647881.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..481
                     /product="(Lyso)-N-acylphosphatidylethanolamine
                     lipase-like isoform X1"
                     /calculated_mol_wt=54000
     Region          125..469
                     /region_name="PLN02894"
                     /note="hydrolase, alpha/beta fold family protein"
                     /db_xref="CDD:215484"
     CDS             1..481
                     /gene="LOC144114265"
                     /coded_by="XM_077647881.1:16..1461"
                     /db_xref="GeneID:144114265"
ORIGIN      
        1 mgthpkqats avllacdasf rarvapdsqg lscnlkarrr wgrlitvpps agrkervraw
       61 sdfgvpfqtp ergqwrsqgg qkaaatgprs spqasftmlt sifrlryyke tlldmvlril
      121 tyfltrftlr lrslfcwcps srqslddaer rvlssmgksy rgyyvdvgri sdspprssrv
      181 wtvhlepded egaealplvl vhgylcgval wvlnmealsr rrrvyaidll gfgkssrptl
      241 ssdagtaeaq maqaleawrr qmgleqfvll ghsfgafvas aytlrhpqhv khliledpwg
      301 fsesrvpdta fhllpwlvta ftvgikffsr adicsvlrag gilgppflgv vlshetnmfr
      361 kivrdetavv syfyhcnvrr psgeasvkal mhrfpwvkhp mgtrlvalpp evgltfiyga
      421 hsfmgrkpaf aireartqsy veihvvedcg hnlhyekpee fcdvvnkace lvdgrlhqsa
      481 d
//
LOCUS       XP_077504008             403 aa            linear   INV 25-NOV-2025
DEFINITION  (Lyso)-N-acylphosphatidylethanolamine lipase-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077504008
VERSION     XP_077504008.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647882.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..403
                     /product="(Lyso)-N-acylphosphatidylethanolamine
                     lipase-like isoform X2"
                     /calculated_mol_wt=45582
     Region          47..391
                     /region_name="PLN02894"
                     /note="hydrolase, alpha/beta fold family protein"
                     /db_xref="CDD:215484"
     CDS             1..403
                     /gene="LOC144114265"
                     /coded_by="XM_077647882.1:75..1286"
                     /db_xref="GeneID:144114265"
ORIGIN      
        1 mcesiglapa iprarkkesr kkpqsrlryy ketlldmvlr iltyfltrft lrlrslfcwc
       61 pssrqsldda errvlssmgk syrgyyvdvg risdspprss rvwtvhlepd edegaealpl
      121 vlvhgylcgv alwvlnmeal srrrrvyaid llgfgkssrp tlssdagtae aqmaqaleaw
      181 rrqmgleqfv llghsfgafv asaytlrhpq hvkhliledp wgfsesrvpd tafhllpwlv
      241 taftvgikff sradicsvlr aggilgppfl gvvlshetnm frkivrdeta vvsyfyhcnv
      301 rrpsgeasvk almhrfpwvk hpmgtrlval ppevgltfiy gahsfmgrkp afaireartq
      361 syveihvved cghnlhyekp eefcdvvnka celvdgrlhq sad
//
LOCUS       XP_077504009             395 aa            linear   INV 25-NOV-2025
DEFINITION  (Lyso)-N-acylphosphatidylethanolamine lipase-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077504009
VERSION     XP_077504009.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647883.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..395
                     /product="(Lyso)-N-acylphosphatidylethanolamine
                     lipase-like isoform X1"
                     /calculated_mol_wt=43842
     Region          9..373
                     /region_name="PLN02894"
                     /note="hydrolase, alpha/beta fold family protein"
                     /db_xref="CDD:215484"
     CDS             1..395
                     /gene="LOC144114266"
                     /coded_by="XM_077647883.1:184..1371"
                     /db_xref="GeneID:144114266"
ORIGIN      
        1 maltmatasc kdgrgpsskg arvsttkgss stpaaageem klarwvpssr erlqeaeekv
       61 fsylkrkytg hyvevgcfgd atspcrmwti tmkpesedsi tqmplvlvhg lgcgsalwvl
      121 nieelsknrv vhavdllgfg rssrpslgnd athieeqmvq sieawrsqmq lerfvllghs
      181 mggflsasys lryphrvahl vlndpwgfpv ydpgrargkr vgswlaplqv fcnhfnvlsg
      241 lrasgllgpf imqaalsgah srfgrvvtdp taipnyvyhc nvrrptgeea frnmsiffgw
      301 tknpmvfrfl eldpavpvtf fygkttfitr spaeyikknr tngyvdlqvi ddcghnvhmd
      361 qpdkfnelvn kvcdlansdm klrpglsdad rpaee
//
LOCUS       XP_077504010             356 aa            linear   INV 25-NOV-2025
DEFINITION  (Lyso)-N-acylphosphatidylethanolamine lipase-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077504010
VERSION     XP_077504010.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647884.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..356
                     /product="(Lyso)-N-acylphosphatidylethanolamine
                     lipase-like isoform X2"
                     /calculated_mol_wt=40102
     Region          2..334
                     /region_name="PLN02894"
                     /note="hydrolase, alpha/beta fold family protein"
                     /db_xref="CDD:215484"
     CDS             1..356
                     /gene="LOC144114266"
                     /coded_by="XM_077647884.1:499..1569"
                     /db_xref="GeneID:144114266"
ORIGIN      
        1 mklarwvpss rerlqeaeek vfsylkrkyt ghyvevgcfg datspcrmwt itmkpeseds
       61 itqmplvlvh glgcgsalwv lnieelsknr vvhavdllgf grssrpslgn dathieeqmv
      121 qsieawrsqm qlerfvllgh smggflsasy slryphrvah lvlndpwgfp vydpgrargk
      181 rvgswlaplq vfcnhfnvls glrasgllgp fimqaalsga hsrfgrvvtd ptaipnyvyh
      241 cnvrrptgee afrnmsiffg wtknpmvfrf leldpavpvt ffygkttfit rspaeyikkn
      301 rtngyvdlqv iddcghnvhm dqpdkfnelv nkvcdlansd mklrpglsda drpaee
//
LOCUS       XP_077504011             408 aa            linear   INV 25-NOV-2025
DEFINITION  (Lyso)-N-acylphosphatidylethanolamine lipase-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077504011
VERSION     XP_077504011.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647885.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..408
                     /product="(Lyso)-N-acylphosphatidylethanolamine
                     lipase-like isoform X1"
                     /calculated_mol_wt=45771
     Region          38..376
                     /region_name="PLN02894"
                     /note="hydrolase, alpha/beta fold family protein"
                     /db_xref="CDD:215484"
     CDS             1..408
                     /gene="LOC144114267"
                     /coded_by="XM_077647885.1:193..1419"
                     /db_xref="GeneID:144114267"
ORIGIN      
        1 mavedqssae lskglrllkh tclladtlls slidlvpswi ssyirwcpts aehleeaerk
       61 llsyvtlpir smfvdvdsil gckgkhqirt lqlgpgrsaa adeervplvl vhgfasgval
      121 wllnldhlaq drttycfdll gfgrssrprl srdpleaeyq fvqsieewra qvgldrfvll
      181 ghsmggflaa syalrfperv ahlvladpwg fperntprpa lqlpswvrvv stllspfnpl
      241 aavriagpwg prlvekirpd igrkyghivn ddqavphyiy hcnaqtpsge safkammtqy
      301 gwarhpmisr igelhqgvpm tfiygsrswv dkqpgirvqr lrpesevdve iidgaghhif
      361 adkpdqfnei vmkicrgada aektirsmva akeqeerdqr emlsqlah
//
LOCUS       XP_077504012             384 aa            linear   INV 25-NOV-2025
DEFINITION  (Lyso)-N-acylphosphatidylethanolamine lipase-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077504012
VERSION     XP_077504012.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647886.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..384
                     /product="(Lyso)-N-acylphosphatidylethanolamine
                     lipase-like isoform X2"
                     /calculated_mol_wt=43154
     Region          7..352
                     /region_name="PLN02894"
                     /note="hydrolase, alpha/beta fold family protein"
                     /db_xref="CDD:215484"
     CDS             1..384
                     /gene="LOC144114267"
                     /coded_by="XM_077647886.1:187..1341"
                     /db_xref="GeneID:144114267"
ORIGIN      
        1 mavedqssae lskgwissyi rwcptsaehl eeaerkllsy vtlpirsmfv dvdsilgckg
       61 khqirtlqlg pgrsaaadee rvplvlvhgf asgvalwlln ldhlaqdrtt ycfdllgfgr
      121 ssrprlsrdp leaeyqfvqs ieewraqvgl drfvllghsm ggflaasyal rfpervahlv
      181 ladpwgfper ntprpalqlp swvrvvstll spfnplaavr iagpwgprlv ekirpdigrk
      241 yghivnddqa vphyiyhcna qtpsgesafk ammtqygwar hpmisrigel hqgvpmtfiy
      301 gsrswvdkqp girvqrlrpe sevdveiidg aghhifadkp dqfneivmki crgadaaekt
      361 irsmvaakeq eerdqremls qlah
//
LOCUS       XP_077504013             443 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin beta-4 chain-like [Amblyomma americanum].
ACCESSION   XP_077504013
VERSION     XP_077504013.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647887.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="tubulin beta-4 chain-like"
                     /calculated_mol_wt=49570
     Region          1..443
                     /region_name="PLN00220"
                     /note="tubulin beta chain; Provisional"
                     /db_xref="CDD:215107"
     CDS             1..443
                     /gene="LOC144114268"
                     /coded_by="XM_077647887.1:103..1434"
                     /db_xref="GeneID:144114268"
ORIGIN      
        1 mreivhiqtg qcgnqigakf wevisdehgi dpsgayhgds dlqlerinvy yneasggkyv
       61 prailvdlep gtmdavrsgq fgqlfrpdnf vfgqngagnn wakghytega elvdsvldvv
      121 rkeaescdcl qgfqlthslg ggtgsgmgtl liskireeyp drimntysvv pspkvsdtvv
      181 epynatlsvh qlventdety cidnealydi cfrtlklttp tygdlnhlvs atmsgvttcl
      241 rfpgqlnadl rklavnmvpf prlhffmtgf apltsrgcqq yraltvpelt qqmfdaknmm
      301 aacdprhgry ltvaavfrgr msmrevddqm lniqnkqssh fvewipdnvk tavcdipprg
      361 lkmsatfign staiqelfrr iseqftamfr rkaflhwytg egmdelefte aesnlnelvs
      421 eyqqyqeapd edegefeetq aea
//
LOCUS       XP_077504014             649 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114269 [Amblyomma americanum].
ACCESSION   XP_077504014
VERSION     XP_077504014.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647888.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..649
                     /product="uncharacterized protein LOC144114269"
                     /calculated_mol_wt=70330
     Region          63..>329
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..649
                     /gene="LOC144114269"
                     /coded_by="XM_077647888.1:131..2080"
                     /db_xref="GeneID:144114269"
ORIGIN      
        1 mpllrlsaal rendslrfns ccpfcifvns saacgfvrep amerrtgqky rtkyaygkrk
       61 rkppipkqkr paatepherr sapisshadv sseaslples atssesggla psashdiavq
      121 yrprhssvfa aasggavpvq rddgevlpkq khavqvhlps esitgsesvg papstsrdia
      181 vqyrpghssv faaasagavp vqrddgevfp kqkrtvqvhl psesmtgses gdpapstsrd
      241 iavqhrpgqs svfaaasdda vpaqrddsel fpkqkrtvqv hleprfvtle aaeeqacsvr
      301 atlravsste gkfgfarqte eesapdedef tliqasvmns iigsllcpkc skkrlsvkhe
      361 tklglaaklv lscgacgpia shwsskrkse grtfevnira mqaiktigkg ptalndfwat
      421 mnvshrglhq ktyqghlnkl fkpgaeeaaq nifadavlav kdvyskmqps spsnitvvyd
      481 gtwltrghss higvgciief ytglvldctv lsnfclgccq qpaesdpsyg twieqhqcqk
      541 ntdvasgqme veaalilfrr sfssyglryt nvicdgdsrt ylalcrdevy gfipltkedc
      601 inhvqkrmgt alrtlvarak kgeplggkgg ltqdlikklt nyygvsaqr
//
LOCUS       XP_077504015             131 aa            linear   INV 25-NOV-2025
DEFINITION  transmembrane protein 170A [Amblyomma americanum].
ACCESSION   XP_077504015
VERSION     XP_077504015.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647889.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..131
                     /product="transmembrane protein 170A"
                     /calculated_mol_wt=14220
     Region          26..131
                     /region_name="Tmemb_170"
                     /note="Putative transmembrane protein 170; pfam10190"
                     /db_xref="CDD:462988"
     CDS             1..131
                     /gene="LOC144114270"
                     /coded_by="XM_077647889.1:374..769"
                     /db_xref="GeneID:144114270"
ORIGIN      
        1 matgtefeta dldsvinvig ltpsdpldtf veiwyqvflw alfssvfvhl vaavvafsml
       61 rkqhigrfsp lvillmglia pmtggaltsa viagvyraar fhmmpfyall wgcgqtvfav
      121 vmsytrvlat l
//
LOCUS       XP_077504016             293 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114271 [Amblyomma americanum].
ACCESSION   XP_077504016
VERSION     XP_077504016.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647890.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..293
                     /product="uncharacterized protein LOC144114271"
                     /calculated_mol_wt=31930
     Region          143..165
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          145..165
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(145,148,161,165)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          186..>252
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          202..222
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(202,205,218,222)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(207,209,211,213..214,217..218,221,235,237,241..242,
                     245..246,249,263,265,267,269..270,273..274)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          215..238
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          230..250
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          258..274
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..293
                     /gene="LOC144114271"
                     /coded_by="XM_077647890.1:375..1256"
                     /db_xref="GeneID:144114271"
ORIGIN      
        1 mpraflitnk rynstvdhdd teqhrpaggr ensperrvst aeapaehead pssrgpeatt
       61 hgcwsawkpa sprspaslsr tatptvseep lsltvhdaqp qlspaaaaas asilaavlqq
      121 vrtlqepgga srlpstagge pyyacpecgk rystssnlar hrqthrsvtd kkarqcphcs
      181 kvyvsmpafs mhvrthgqgc qcpfcgkcfs rpwllqghir thtgekpfkc sqcskafadk
      241 snlrahvqth sptkpfvcrr cgkafalksy lykheesscm rvraaaptte hda
//
LOCUS       XP_077504017             196 aa            linear   INV 25-NOV-2025
DEFINITION  peptidyl-prolyl cis-trans isomerase B-like [Amblyomma americanum].
ACCESSION   XP_077504017
VERSION     XP_077504017.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647891.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..196
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..196
                     /product="peptidyl-prolyl cis-trans isomerase B-like"
                     /calculated_mol_wt=21170
     Region          29..185
                     /region_name="cyclophilin"
                     /note="cyclophilin-type peptidylprolyl cis- trans
                     isomerases. This family contains eukaryotic, bacterial and
                     archeal proteins which exhibit a peptidylprolyl cis- trans
                     isomerases activity (PPIase, Rotamase) and in addition
                     bind the immunosuppressive drug...; cl00197"
                     /db_xref="CDD:469651"
     Site            order(81,83,86..87,89,127..128,137,139,147..148,152)
                     /site_type="active"
                     /db_xref="CDD:238194"
     CDS             1..196
                     /gene="LOC144114273"
                     /coded_by="XM_077647891.1:112..702"
                     /db_xref="GeneID:144114273"
ORIGIN      
        1 mskvicfivl lttllgsvlc adpdktithk vnfditigdk dagrvvigly gkvapktvrn
       61 fvafageghq glkyegtmfh rvikgfmiqg gdvqklegrg sisiygryfd detfalthdg
      121 pgtlsmanag kntngaqffi tteatpwldg hhvvfgkvid gldvvrkven tettrsdapv
      181 eqvlikrssv dtlvpi
//
LOCUS       XP_077504018             409 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier family 35 member C2 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504018
VERSION     XP_077504018.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647892.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..409
                     /product="solute carrier family 35 member C2 isoform X1"
                     /calculated_mol_wt=45888
     Region          116..360
                     /region_name="TPT_S35C2"
                     /note="solute carrier family 35 member C2, member of the
                     triose-phosphate transporter family; cd21092"
                     /db_xref="CDD:411044"
     CDS             1..409
                     /gene="LOC144114274"
                     /coded_by="XM_077647892.1:261..1490"
                     /db_xref="GeneID:144114274"
ORIGIN      
        1 msasacnves ndgakrygcf gsyqrlhsaa nhslqparyr mhalrysaia lrtvgfvlly
       61 ycfsigitfy qkwfikefrf pltvvichlv vkfvlsgllr lvyqvctgkp rillgwavyv
      121 kqlaitgvas aldigfsnws fefitvslyt mtkstciifi lgfslvfgle rrrcslvfiv
      181 llialglfmf tyqstqfste gfllvlsasf laglrwtlaq lvmqrkevgl gnpidmifhv
      241 qpwmilgllp laivfegipi atsekvfrfr daevvlrtck yvlagsllaf lmelseylll
      301 tytssltlsi agiikaictl ylavkysgde mssmnllgli icllgialhi vvkslntnae
      361 edtlyehmgs kqnllnaine dcedsdeevc hfemqpltrr qqaqrtaql
//
LOCUS       XP_077504019             409 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier family 35 member C2 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504019
VERSION     XP_077504019.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647893.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..409
                     /product="solute carrier family 35 member C2 isoform X2"
                     /calculated_mol_wt=45932
     Region          116..360
                     /region_name="TPT_S35C2"
                     /note="solute carrier family 35 member C2, member of the
                     triose-phosphate transporter family; cd21092"
                     /db_xref="CDD:411044"
     CDS             1..409
                     /gene="LOC144114274"
                     /coded_by="XM_077647893.1:261..1490"
                     /db_xref="GeneID:144114274"
ORIGIN      
        1 msasacnves ndgakrygcf gsyqrlhsaa nhslqparyr mhalrysaia lrtvgfvlly
       61 ycfsigitfy qkwfikefrf pltvvichlv vkfvlsgllr lvyqvctgkp rillgwavyv
      121 kqlaitgvas aldigfsnws fefitvslyt mtkstciifi lgfslvfgle rrrcslvfiv
      181 llialglfmf tyqstqfste gfllvlsasf laglrwtlaq lvmqrkevgl gnpidmifhv
      241 qpwmilgllp laivfegipi atsekvfrfr daevvlrtck yvlagsllaf lmelseylll
      301 tytssltlsi agiikevctl ylavkysgde mssmnllgli icllgialhi vvkslntnae
      361 edtlyehmgs kqnllnaine dcedsdeevc hfemqpltrr qqaqrtaql
//
LOCUS       XP_077504020             401 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier family 35 member C2 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504020
VERSION     XP_077504020.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647894.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..401
                     /product="solute carrier family 35 member C2 isoform X3"
                     /calculated_mol_wt=44726
     Region          116..359
                     /region_name="TPT_S35C2"
                     /note="solute carrier family 35 member C2, member of the
                     triose-phosphate transporter family; cd21092"
                     /db_xref="CDD:411044"
     CDS             1..401
                     /gene="LOC144114274"
                     /coded_by="XM_077647894.1:261..1466"
                     /db_xref="GeneID:144114274"
ORIGIN      
        1 msasacnves ndgakrygcf gsyqrlhsaa nhslqparyr mhalrysaia lrtvgfvlly
       61 ycfsigitfy qkwfikefrf pltvvichlv vkfvlsgllr lvyqvctgkp rillgwavyv
      121 kqlaitgvas aldigfsnws fefitvslyt mtkstciifi lgfslvfgle rrrcslvfiv
      181 llialglfmf tyqstqfste gfllvlsasf laglrwtlaq lvmqrkevgl gnpidmifhv
      241 qpwmilgllp laivfegipi atsekvfrfr daevvlrtck yvlagsllaf lmelseylll
      301 tytssltlsi agiikaictl ylavkysgde mssmnllgli icllgialhi vvkslntngl
      361 gldsimrvtc mllpgsllpl ltcrkrkrip ftsiwgqski c
//
LOCUS       XP_077504022             401 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier family 35 member C2 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077504022
VERSION     XP_077504022.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647896.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..401
                     /product="solute carrier family 35 member C2 isoform X4"
                     /calculated_mol_wt=44770
     Region          116..359
                     /region_name="TPT_S35C2"
                     /note="solute carrier family 35 member C2, member of the
                     triose-phosphate transporter family; cd21092"
                     /db_xref="CDD:411044"
     CDS             1..401
                     /gene="LOC144114274"
                     /coded_by="XM_077647896.1:282..1487"
                     /db_xref="GeneID:144114274"
ORIGIN      
        1 msasacnves ndgakrygcf gsyqrlhsaa nhslqparyr mhalrysaia lrtvgfvlly
       61 ycfsigitfy qkwfikefrf pltvvichlv vkfvlsgllr lvyqvctgkp rillgwavyv
      121 kqlaitgvas aldigfsnws fefitvslyt mtkstciifi lgfslvfgle rrrcslvfiv
      181 llialglfmf tyqstqfste gfllvlsasf laglrwtlaq lvmqrkevgl gnpidmifhv
      241 qpwmilgllp laivfegipi atsekvfrfr daevvlrtck yvlagsllaf lmelseylll
      301 tytssltlsi agiikevctl ylavkysgde mssmnllgli icllgialhi vvkslntngl
      361 gldsimrvtc mllpgsllpl ltcrkrkrip ftsiwgqski c
//
LOCUS       XP_077504023             400 aa            linear   INV 25-NOV-2025
DEFINITION  cyclin E [Amblyomma americanum].
ACCESSION   XP_077504023
VERSION     XP_077504023.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647897.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..400
                     /product="cyclin E"
                     /calculated_mol_wt=44487
     Region          102..231
                     /region_name="CYCLIN_CCNE_rpt1"
                     /note="first cyclin box found in G1/S-specific cyclin-E
                     (CCNE) family; cd20519"
                     /db_xref="CDD:410223"
     Site            order(102,104,107..108,111..112,115,119,154,189,193..197,
                     201..202,219,222..223,226..227,230..231)
                     /site_type="other"
                     /note="CDK interface [polypeptide binding]"
                     /db_xref="CDD:410223"
     Region          235..336
                     /region_name="CYCLIN_CCNE_rpt2"
                     /note="second cyclin box found in G1/S-specific cyclin-E
                     (CCNE) family; cd20520"
                     /db_xref="CDD:410224"
     Site            order(236,243..244,247,263..265,267)
                     /site_type="other"
                     /note="CDK interface [polypeptide binding]"
                     /db_xref="CDD:410224"
     CDS             1..400
                     /gene="CycE"
                     /coded_by="XM_077647897.1:252..1454"
                     /db_xref="GeneID:144114275"
ORIGIN      
        1 mskrntkttr skrsrgsmgs imlrkkrrtp gtiedvqssd vnesfeeats mqasfgtsdr
       61 sskpyfgsag tpksyshfrg ihslptpepr tsrnsplpqf dwadqenvwg lmirkdiiyv
      121 hsasvlerhp alqprmrail ldwlievcev yrlhrdtyyl aqdivdryla kssnlpknql
      181 qllgitalfl sskmeeiypp klaefayvtd gacqeaeild keltilvaln wdltpvtvng
      241 wlntylqvaa kkekknkede nflypgfsac sfiqvaqlvd lcmldvdclq ftysiiaasa
      301 ihhmmspala savsgykave laaciewmvp fatvvkglke ttgtsgksgh eiqvhhvnlq
      361 lvekaiclkk eanasleskr dkvtnggnav lglltpppvq
//
LOCUS       XP_077504024             204 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114276 [Amblyomma americanum].
ACCESSION   XP_077504024
VERSION     XP_077504024.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647898.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..204
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..204
                     /product="uncharacterized protein LOC144114276"
                     /calculated_mol_wt=22571
     CDS             1..204
                     /gene="LOC144114276"
                     /coded_by="XM_077647898.1:1308..1922"
                     /db_xref="GeneID:144114276"
ORIGIN      
        1 msrakttvfv iiinssslcp lqdkgfshts pvnpvlfqlw ppypckllyl irlvmlsvki
       61 hhtkrnfmys anidwysmcs gtskcqridg kqvphhagcw aaaafpgpat estlspgqaq
      121 nerlgerrcs llyraigyll ppyglatlpf tansgshfvv rratwatssi rllkkgiipa
      181 dtvghdtaam hpysrmllvq lhft
//
LOCUS       XP_077504025             452 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114277 [Amblyomma americanum].
ACCESSION   XP_077504025
VERSION     XP_077504025.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647899.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..452
                     /product="uncharacterized protein LOC144114277"
                     /calculated_mol_wt=47189
     Region          280..365
                     /region_name="ETS"
                     /note="erythroblast transformation specific domain;
                     smart00413"
                     /db_xref="CDD:197710"
     CDS             1..452
                     /gene="LOC144114277"
                     /coded_by="XM_077647899.1:263..1621"
                     /db_xref="GeneID:144114277"
ORIGIN      
        1 mleassacpl gdlgkvvkle ewaaaaaatp aayryphhlh hhappqpgps pagrqvgrya
       61 agsgvpgepp lkgspgpvds sllasfrdry lgasappfgg mgpflgpppp phflgsaaes
      121 atakgtpepa rrpagrgsas aspaspaggs lapppatrpg pppaasaadk rhfaasdpps
      181 gggggtaann dagggratak ayastpdshp lrltkdetsy kaawgspspt qaqgyphnal
      241 pampknalda hthnhlrnmq dpyqvfgpts srlassgsgq iqlwqfllel lsdssnasci
      301 twegtngefk ltdpdevarr wgerkskpnm nydklsralr yyydknimtk vhgkryayrf
      361 dfqglaqatq ppsaaaaaaa aadpaaykyh hhqssasdlf gyhaspklnf psttgslfps
      421 pspywtspnn lyasshhsmp hpghigsyph ya
//
LOCUS       XP_077504026             452 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114277 [Amblyomma americanum].
ACCESSION   XP_077504026
VERSION     XP_077504026.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647900.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..452
                     /product="uncharacterized protein LOC144114277"
                     /calculated_mol_wt=47189
     Region          280..365
                     /region_name="ETS"
                     /note="erythroblast transformation specific domain;
                     smart00413"
                     /db_xref="CDD:197710"
     CDS             1..452
                     /gene="LOC144114277"
                     /coded_by="XM_077647900.1:211..1569"
                     /db_xref="GeneID:144114277"
ORIGIN      
        1 mleassacpl gdlgkvvkle ewaaaaaatp aayryphhlh hhappqpgps pagrqvgrya
       61 agsgvpgepp lkgspgpvds sllasfrdry lgasappfgg mgpflgpppp phflgsaaes
      121 atakgtpepa rrpagrgsas aspaspaggs lapppatrpg pppaasaadk rhfaasdpps
      181 gggggtaann dagggratak ayastpdshp lrltkdetsy kaawgspspt qaqgyphnal
      241 pampknalda hthnhlrnmq dpyqvfgpts srlassgsgq iqlwqfllel lsdssnasci
      301 twegtngefk ltdpdevarr wgerkskpnm nydklsralr yyydknimtk vhgkryayrf
      361 dfqglaqatq ppsaaaaaaa aadpaaykyh hhqssasdlf gyhaspklnf psttgslfps
      421 pspywtspnn lyasshhsmp hpghigsyph ya
//
LOCUS       XP_077504027             605 aa            linear   INV 25-NOV-2025
DEFINITION  RING finger and SPRY domain-containing protein 1-like [Amblyomma
            americanum].
ACCESSION   XP_077504027
VERSION     XP_077504027.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647901.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..605
                     /product="RING finger and SPRY domain-containing protein
                     1-like"
                     /calculated_mol_wt=67223
     Region          390..510
                     /region_name="SPRY_RING"
                     /note="SPRY domain at N-terminus of Really Interesting New
                     Gene (RING) finger domain; cd12883"
                     /db_xref="CDD:293941"
     Region          555..597
                     /region_name="RING-HC_RSPRY1"
                     /note="RING finger, HC subclass, found in RING finger and
                     SPRY domain-containing protein 1 (RSPRY1) and similar
                     proteins; cd16566"
                     /db_xref="CDD:438228"
     CDS             1..605
                     /gene="LOC144114278"
                     /coded_by="XM_077647901.1:149..1966"
                     /db_xref="GeneID:144114278"
ORIGIN      
        1 mvylttvask pqqgdlsalc slrrsstlav alvtcrgpaa ahtvferaak atgaamgnll
       61 ccvcrdhksg vddggnvggr ygfhrrqssr slpveahdgs nerrrsssaf swhgtrgsis
      121 apstsyvehl iletlsiirt lvdneqeppp fmiklhliad kesgwllvvr smvnaipmdd
      181 plgpavitli lddcplptke svgklsqmfq lsrelsisgr nnptwhrnic vvlgciaekl
      241 agpssvallt edtleylian ldpvtcntlv ilfslialek faqtsenkvt lekrlaslre
      301 nplakmepwv ssedfverqv gfcaqwcldn lfilegrryt yevtdrtnin amlnsndvse
      361 ylkissnglv arcdassfes vrctfqvdsg vwyyealivt sgvmqigwat reskflnheg
      421 ygigddefsi aydgcrqliw ynalsqshsh pcwkpgdilg tlldmkafqi tfylngeplp
      481 pntqvfqnsk sgffaaasfm tfqqcefnfg rspfkfppsg vqfscfnsfa hlseedkvil
      541 prhirmeklr hssvredsct lcfdrsaavr lepcqhkgfc secamqlevc pmcrseirer
      601 ieasl
//
LOCUS       XP_077504028             384 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: sorting nexin 21 [Amblyomma americanum].
ACCESSION   XP_077504028
VERSION     XP_077504028.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647902.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..384
                     /product="LOW QUALITY PROTEIN: sorting nexin 21"
                     /calculated_mol_wt=42427
     Region          76..184
                     /region_name="PX_domain"
                     /note="The Phox Homology domain, a phosphoinositide
                     binding module; cl02563"
                     /db_xref="CDD:470617"
     Site            order(115..117,144..145,158)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:132768"
     Region          196..255
                     /region_name="TPR_12"
                     /note="Tetratricopeptide repeat; pfam13424"
                     /db_xref="CDD:315987"
     CDS             1..384
                     /gene="Snx21"
                     /coded_by="XM_077647902.1:366..1520"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:144114280"
ORIGIN      
        1 mtseelnssl sddldelrcs qvvgtlcfse aaddagecfv sstsrtirsw sktsqtntlm
       61 asagdppsrv gpqqvvfeiv saktvvegrk kfvcytvlvk kspglerlpg vlerrysdfs
      121 alfaglrrrh pscvalrdfp fprkallgnf ttevitersl afrrllsrvh aspelrrspe
      181 faeftwrrev crahrlmaag qfedasvlle naysvqekvl gdgdpdtftt lavltaclna
      241 vdnvaeaqky aelalskrlp ggeatsasdl evpllvlair lwwavgkekr eleerlrqvk
      301 dtglnvdalp tllelvlkkd sapcihlrle latitcfsqg vtwcttpvwp tfecfislfl
      361 srigsgiyfl cavdldsfav vtic
//
LOCUS       XP_077504029             332 aa            linear   INV 25-NOV-2025
DEFINITION  docking protein 5-like [Amblyomma americanum].
ACCESSION   XP_077504029
VERSION     XP_077504029.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647903.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..332
                     /product="docking protein 5-like"
                     /calculated_mol_wt=36809
     Region          10..113
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          132..234
                     /region_name="PTB_DOK4_DOK5_DOK6"
                     /note="Downstream of tyrosine kinase 4, 5, and 6 proteins
                     phosphotyrosine-binding domain (PTBi); cd13164"
                     /db_xref="CDD:241318"
     Site            order(183..188,194,198..199,209,222)
                     /site_type="other"
                     /note="putative phosphopeptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:241318"
     CDS             1..332
                     /gene="LOC144114281"
                     /coded_by="XM_077647903.1:368..1366"
                     /db_xref="GeneID:144114281"
ORIGIN      
        1 matdtsdiak qgyvrvrsrn igvwqrrwlv lrkatsttac rlekyleekh arahtgqkvv
       61 lltpatsitr lpssvrkhaf sicfqdgsvk svacdsdlea dtwvklmvqe clepqsglsa
      121 gepdilspgi qkelqeqfhv ylvpspkldv fgecllqvth ehiylwdlls prtklaawpl
      181 talrrygsdp akftfeagrh catgegmfvf htlegekiyc kvhqatlaia eahhrsrrlq
      241 avpsappqsl ipptgpasvp yrnstsplqq fsssenlpgs qdrtshslmp lrdmpttsqs
      301 fddilnpprv sttvggasrc slrshllqfq ar
//
LOCUS       XP_077504030             297 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114282 [Amblyomma americanum].
ACCESSION   XP_077504030
VERSION     XP_077504030.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647904.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..297
                     /product="uncharacterized protein LOC144114282"
                     /calculated_mol_wt=33550
     Region          25..258
                     /region_name="ICMT"
                     /note="Isoprenylcysteine carboxyl methyltransferase (ICMT)
                     family; cl21511"
                     /db_xref="CDD:473892"
     CDS             1..297
                     /gene="LOC144114282"
                     /coded_by="XM_077647904.1:194..1087"
                     /db_xref="GeneID:144114282"
ORIGIN      
        1 maveildvdh laisaivtvg lqfiffviaa tfhfdkltdf agganfiila ltvfllaqty
       61 sprqclvtgl vclwgvrlst fllfriikig rderfqevgk nvvrfavfwt fqavwvytvs
      121 lpvifvnspr kaepltdatm trldmagtiv fvigflceam adaqkytyrq ntsnarhwcd
      181 vglwkysrhp nyfgeitlww gifliatnvl kgaewvavls plfittiilf lsgvpllens
      241 adirygcmee yqrykrstsp llplppglyr evpscfkcli cfefpiytns kaiatsi
//
LOCUS       XP_077504032             297 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114282 [Amblyomma americanum].
ACCESSION   XP_077504032
VERSION     XP_077504032.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647906.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..297
                     /product="uncharacterized protein LOC144114282"
                     /calculated_mol_wt=33550
     Region          25..258
                     /region_name="ICMT"
                     /note="Isoprenylcysteine carboxyl methyltransferase (ICMT)
                     family; cl21511"
                     /db_xref="CDD:473892"
     CDS             1..297
                     /gene="LOC144114282"
                     /coded_by="XM_077647906.1:176..1069"
                     /db_xref="GeneID:144114282"
ORIGIN      
        1 maveildvdh laisaivtvg lqfiffviaa tfhfdkltdf agganfiila ltvfllaqty
       61 sprqclvtgl vclwgvrlst fllfriikig rderfqevgk nvvrfavfwt fqavwvytvs
      121 lpvifvnspr kaepltdatm trldmagtiv fvigflceam adaqkytyrq ntsnarhwcd
      181 vglwkysrhp nyfgeitlww gifliatnvl kgaewvavls plfittiilf lsgvpllens
      241 adirygcmee yqrykrstsp llplppglyr evpscfkcli cfefpiytns kaiatsi
//
LOCUS       XP_077504033             172 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin-conjugating enzyme variant 1A isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504033
VERSION     XP_077504033.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647907.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..172
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..172
                     /product="ubiquitin-conjugating enzyme variant 1A isoform
                     X1"
                     /calculated_mol_wt=19321
     Region          36..169
                     /region_name="UEV_UBE2V"
                     /note="ubiquitin E2 variant (UEV) domain of
                     ubiquitin-conjugating enzyme E2 variant 1, 2 and related
                     proteins; cd23807"
                     /db_xref="CDD:467427"
     CDS             1..172
                     /gene="Uev1A"
                     /coded_by="XM_077647907.1:16..534"
                     /db_xref="GeneID:144114283"
ORIGIN      
        1 mvaamclhtv lvysrcrilf rgslikmisa chsggvvvpr nfrlleeleq gqkgvgdgti
       61 swgleddcdm tlthwtgmii gpprtpyenr iyslrvecgs qypdeppcvk fiskikmvgi
      121 sdsnggverr slnvlnrwqr sysiktvlqe lrramtlken qrmaqppegt tf
//
LOCUS       XP_077504034             168 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin-conjugating enzyme variant 1A isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504034
VERSION     XP_077504034.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647908.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..168
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..168
                     /product="ubiquitin-conjugating enzyme variant 1A isoform
                     X2"
                     /calculated_mol_wt=18829
     Region          22..>111
                     /region_name="UEV_UBE2V"
                     /note="ubiquitin E2 variant (UEV) domain of
                     ubiquitin-conjugating enzyme E2 variant 1, 2 and related
                     proteins; cd23807"
                     /db_xref="CDD:467427"
     CDS             1..168
                     /gene="Uev1A"
                     /coded_by="XM_077647908.1:101..607"
                     /db_xref="GeneID:144114283"
ORIGIN      
        1 maastcaaec gektnrkppg gvvvprnfrl leeleqgqkg vgdgtiswgl eddcdmtlth
       61 wtgmiigppr tpyenriysl rvecgsqypd eppcvkfisk ikmvgisdsn ggknckrsps
      121 gckmyqkkai fqteaktrvs hqklwsiefc kypkrrghik vhrffrst
//
LOCUS       XP_077504035             158 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin-conjugating enzyme variant 1A isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504035
VERSION     XP_077504035.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647909.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="ubiquitin-conjugating enzyme variant 1A isoform
                     X3"
                     /calculated_mol_wt=17575
     Region          22..155
                     /region_name="UEV_UBE2V"
                     /note="ubiquitin E2 variant (UEV) domain of
                     ubiquitin-conjugating enzyme E2 variant 1, 2 and related
                     proteins; cd23807"
                     /db_xref="CDD:467427"
     CDS             1..158
                     /gene="Uev1A"
                     /coded_by="XM_077647909.1:100..576"
                     /db_xref="GeneID:144114283"
ORIGIN      
        1 maastcaaec gektnrkppg gvvvprnfrl leeleqgqkg vgdgtiswgl eddcdmtlth
       61 wtgmiigppr tpyenriysl rvecgsqypd eppcvkfisk ikmvgisdsn ggverrslnv
      121 lnrwqrsysi ktvlqelrra mtlkenqrma qppegttf
//
LOCUS       XP_077504036             157 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin-conjugating enzyme variant 1A isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077504036
VERSION     XP_077504036.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647910.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..157
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..157
                     /product="ubiquitin-conjugating enzyme variant 1A isoform
                     X4"
                     /calculated_mol_wt=17588
     Region          11..>100
                     /region_name="UEV_UBE2V"
                     /note="ubiquitin E2 variant (UEV) domain of
                     ubiquitin-conjugating enzyme E2 variant 1, 2 and related
                     proteins; cd23807"
                     /db_xref="CDD:467427"
     CDS             1..157
                     /gene="Uev1A"
                     /coded_by="XM_077647910.1:127..600"
                     /db_xref="GeneID:144114283"
ORIGIN      
        1 maastcaagg vvvprnfrll eeleqgqkgv gdgtiswgle ddcdmtlthw tgmiigpprt
       61 pyenriyslr vecgsqypde ppcvkfiski kmvgisdsng gknckrspsg ckmyqkkaif
      121 qteaktrvsh qklwsiefck ypkrrghikv hrffrst
//
LOCUS       XP_077504037             147 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin-conjugating enzyme variant 1A isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077504037
VERSION     XP_077504037.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647911.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..147
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..147
                     /product="ubiquitin-conjugating enzyme variant 1A isoform
                     X5"
                     /calculated_mol_wt=16335
     Region          11..144
                     /region_name="UEV_UBE2V"
                     /note="ubiquitin E2 variant (UEV) domain of
                     ubiquitin-conjugating enzyme E2 variant 1, 2 and related
                     proteins; cd23807"
                     /db_xref="CDD:467427"
     CDS             1..147
                     /gene="Uev1A"
                     /coded_by="XM_077647911.1:123..566"
                     /db_xref="GeneID:144114283"
ORIGIN      
        1 maastcaagg vvvprnfrll eeleqgqkgv gdgtiswgle ddcdmtlthw tgmiigpprt
       61 pyenriyslr vecgsqypde ppcvkfiski kmvgisdsng gverrslnvl nrwqrsysik
      121 tvlqelrram tlkenqrmaq ppegttf
//
LOCUS       XP_077504038             397 aa            linear   INV 25-NOV-2025
DEFINITION  obg-like ATPase 1 [Amblyomma americanum].
ACCESSION   XP_077504038
VERSION     XP_077504038.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647912.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..397
                     /product="obg-like ATPase 1"
                     /calculated_mol_wt=44808
     Region          1..388
                     /region_name="PTZ00258"
                     /note="GTP-binding protein; Provisional"
                     /db_xref="CDD:240334"
     CDS             1..397
                     /gene="LOC144114284"
                     /coded_by="XM_077647912.1:161..1354"
                     /db_xref="GeneID:144114284"
ORIGIN      
        1 mppkkkedet erpvligrvg tnlkvgivgl pnvgkstffn vltanqvpae nfpfctidpn
       61 esrvavpdar feylceyfkp askvpaflnv vdiaglvkga sdgqglgnaf lshiracdal
      121 fhlcrtfede dvthvegdvn pvrdidiine elrkkdeeyl laaidkmert vlrgadkkmk
      181 peydilikir gvlcdekkhl rfcdwnvnei evlnkhlfit akpmiylvnl aekdfirkkn
      241 kwlpkikewv dkndagaavi pfsgafehkl iemdaeqkka yeeehkvtsv ldkiivtgyk
      301 alqlmyffta gkdevkawti qkhtkapqaa gkihtdfekg fimaevmkfd dfkeegseaa
      361 vkasgkyrqq grnyvvedgd iiffkfnaga glqakkk
//
LOCUS       XP_077504039             273 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114285 [Amblyomma americanum].
ACCESSION   XP_077504039
VERSION     XP_077504039.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647913.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..273
                     /product="uncharacterized protein LOC144114285"
                     /calculated_mol_wt=30453
     Region          6..>111
                     /region_name="SWIM"
                     /note="Uncharacterized protein, contains SWIM-type Zn
                     finger domain [Function unknown]; COG4715"
                     /db_xref="CDD:443750"
     CDS             1..273
                     /gene="LOC144114285"
                     /coded_by="XM_077647913.1:346..1167"
                     /db_xref="GeneID:144114285"
ORIGIN      
        1 mysveqlied sdvcvqakcv sqvsdhvyvt ydvhlltwsl hakqelrikd grctcnagia
       61 aqckhaaavc fyinryedts ctsqlqqwgk psmkphrprn qdlmaffpsq esnstntkaa
      121 spsillnytd iissmkeils mgqmnaeklv cqdvlnkivd qaskvadeei wegplklttw
      181 ravyhclkvv nnsatkkkim crdlaiemns leaaryhtdv vlscspckls vltqgqaksp
      241 rwlqnadigl aapkhivyap kvvkmatrkl lsn
//
LOCUS       XP_077504040            1057 aa            linear   INV 25-NOV-2025
DEFINITION  serine/threonine-protein phosphatase 4 regulatory subunit 1-like
            isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504040
VERSION     XP_077504040.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647914.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1057
                     /product="serine/threonine-protein phosphatase 4
                     regulatory subunit 1-like isoform X1"
                     /calculated_mol_wt=116423
     Region          151..183
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          201..229
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(217,222,225,228..229,259..260,263,266..267,270,
                     298..299,302,305..306,309,336..337,340,343..344,375..376,
                     379,382)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          241..270
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          280..310
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          319..344
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          355..382
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          576..>600
                     /region_name="WRNPLPNID"
                     /note="Putative WW-binding domain and destruction box;
                     pfam15017"
                     /db_xref="CDD:464448"
     Site            order(803..804,807,810..811,882..883,886,889..890,893,
                     921..922,925,928..929,932,968..969,972,1002..1003,1006)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          825..893
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          828..853
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          903..933
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          943..973
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          982..1008
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..1057
                     /gene="LOC144114287"
                     /coded_by="XM_077647914.1:162..3335"
                     /db_xref="GeneID:144114287"
ORIGIN      
        1 mpddgdvlvp dqrtsgeads vnpgnegrtd evihapvevr rarvgsinvv rpssrrssrr
       61 rssrlvhgas scwsnfslam ltadqeetsa eqigegcedd daasrrqell eepnpvvkle
      121 ryaysdivfn rqlvarnivd tlravvdsqe avdavltals slsldcepsv rtelveqlpn
      181 iasfcasegg llqnvvrehv vpilvqyltd vanqvrkrtq vsllfilera lidrkqleeq
      241 icslvvhlte senvdeyrme avtlmtkmap yvgkdttayl flprfislcq ddsfhvrkvc
      301 aanfgdfssi vgqkytedni pvfqmlcedc vwgvrkacae vfmpvscics latrrnrltp
      361 lfgsllddqs rwvrsaayqg lgpfistfad plrtglycse dgvvsmrvsd gppeacsdee
      421 aaspaetavs lkgtgiqeca vtlvppaadp vnfgkvgmie datsallekg qqqiafdddk
      481 gepcgasscm asvedscghk ncaamslpkk ledastgcta etvfdgalaa pkqfegsliq
      541 kdvpgdqqgc tetlkalsve gdrqrlvvhv dsgsdsdfna fqfwrmpipe levdieflgn
      601 rptavhvrak sqsqgnrcfs sdlsvevlgd ngkpintsgd ldlnqdgais sqrlsgdstl
      661 rirtaslssl tsadqecnil lsssvteasv afneagevag vhkvrmsnsk evagtentll
      721 akslntssds wsaheqdivp vllleryqsm tdptlaqnld sdigrhcahs lpavaltlgr
      781 qfwpclrtty rclvsdvqwk vrhivasclh elatilgpei asqdlvpsfe gfvkdvdevr
      841 vgllerlalf lrglskadqr rllplladfl cmdnqrswrf rftlarqlke iaalyspldv
      901 hqylvpmvse llvdkvaevr hisveamvav lqqlaphylm ckellqqlce kaqdrkwcqr
      961 qlfcelaeqm isaeclsgee figtvlpkll clrhdsvpnv rmalarclvr gvwpkaffwd
     1021 sgniykdelk atlaslqedt drdvrfyaql ypkdsvd
//
LOCUS       XP_077504041            1047 aa            linear   INV 25-NOV-2025
DEFINITION  serine/threonine-protein phosphatase 4 regulatory subunit 1-like
            isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504041
VERSION     XP_077504041.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647915.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1047
                     /product="serine/threonine-protein phosphatase 4
                     regulatory subunit 1-like isoform X2"
                     /calculated_mol_wt=115243
     Region          141..173
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          191..219
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(207,212,215,218..219,249..250,253,256..257,260,
                     288..289,292,295..296,299,326..327,330,333..334,365..366,
                     369,372)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          231..260
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          270..300
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          309..334
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          345..372
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          566..>590
                     /region_name="WRNPLPNID"
                     /note="Putative WW-binding domain and destruction box;
                     pfam15017"
                     /db_xref="CDD:464448"
     Site            order(793..794,797,800..801,872..873,876,879..880,883,
                     911..912,915,918..919,922,958..959,962,992..993,996)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          815..883
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          818..843
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          893..923
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          933..963
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          972..998
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..1047
                     /gene="LOC144114287"
                     /coded_by="XM_077647915.1:16..3159"
                     /db_xref="GeneID:144114287"
ORIGIN      
        1 magtsgeads vnpgnegrtd evihapvevr rarvgsinvv rpssrrssrr rssrlvhgas
       61 scwsnfslam ltadqeetsa eqigegcedd daasrrqell eepnpvvkle ryaysdivfn
      121 rqlvarnivd tlravvdsqe avdavltals slsldcepsv rtelveqlpn iasfcasegg
      181 llqnvvrehv vpilvqyltd vanqvrkrtq vsllfilera lidrkqleeq icslvvhlte
      241 senvdeyrme avtlmtkmap yvgkdttayl flprfislcq ddsfhvrkvc aanfgdfssi
      301 vgqkytedni pvfqmlcedc vwgvrkacae vfmpvscics latrrnrltp lfgsllddqs
      361 rwvrsaayqg lgpfistfad plrtglycse dgvvsmrvsd gppeacsdee aaspaetavs
      421 lkgtgiqeca vtlvppaadp vnfgkvgmie datsallekg qqqiafdddk gepcgasscm
      481 asvedscghk ncaamslpkk ledastgcta etvfdgalaa pkqfegsliq kdvpgdqqgc
      541 tetlkalsve gdrqrlvvhv dsgsdsdfna fqfwrmpipe levdieflgn rptavhvrak
      601 sqsqgnrcfs sdlsvevlgd ngkpintsgd ldlnqdgais sqrlsgdstl rirtaslssl
      661 tsadqecnil lsssvteasv afneagevag vhkvrmsnsk evagtentll akslntssds
      721 wsaheqdivp vllleryqsm tdptlaqnld sdigrhcahs lpavaltlgr qfwpclrtty
      781 rclvsdvqwk vrhivasclh elatilgpei asqdlvpsfe gfvkdvdevr vgllerlalf
      841 lrglskadqr rllplladfl cmdnqrswrf rftlarqlke iaalyspldv hqylvpmvse
      901 llvdkvaevr hisveamvav lqqlaphylm ckellqqlce kaqdrkwcqr qlfcelaeqm
      961 isaeclsgee figtvlpkll clrhdsvpnv rmalarclvr gvwpkaffwd sgniykdelk
     1021 atlaslqedt drdvrfyaql ypkdsvd
//
LOCUS       XP_077504042            1015 aa            linear   INV 25-NOV-2025
DEFINITION  serine/threonine-protein phosphatase 4 regulatory subunit 1-like
            isoform X3 [Amblyomma americanum].
ACCESSION   XP_077504042
VERSION     XP_077504042.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647916.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1015
                     /product="serine/threonine-protein phosphatase 4
                     regulatory subunit 1-like isoform X3"
                     /calculated_mol_wt=111691
     Region          109..141
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          159..187
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(175,180,183,186..187,217..218,221,224..225,228,
                     256..257,260,263..264,267,294..295,298,301..302,333..334,
                     337,340)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          199..228
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          238..268
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          277..302
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          313..340
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          534..>558
                     /region_name="WRNPLPNID"
                     /note="Putative WW-binding domain and destruction box;
                     pfam15017"
                     /db_xref="CDD:464448"
     Site            order(761..762,765,768..769,840..841,844,847..848,851,
                     879..880,883,886..887,890,926..927,930,960..961,964)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          783..851
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          786..811
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          861..891
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          901..931
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          940..966
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..1015
                     /gene="LOC144114287"
                     /coded_by="XM_077647916.1:165..3212"
                     /db_xref="GeneID:144114287"
ORIGIN      
        1 mpddgdvlvp dqrtsgeads vnpgneassc wsnfslamlt adqeetsaeq igegceddda
       61 asrrqellee pnpvvklery aysdivfnrq lvarnivdtl ravvdsqeav davltalssl
      121 sldcepsvrt elveqlpnia sfcaseggll qnvvrehvvp ilvqyltdva nqvrkrtqvs
      181 llfilerali drkqleeqic slvvhltese nvdeyrmeav tlmtkmapyv gkdttaylfl
      241 prfislcqdd sfhvrkvcaa nfgdfssivg qkytednipv fqmlcedcvw gvrkacaevf
      301 mpvscicsla trrnrltplf gsllddqsrw vrsaayqglg pfistfadpl rtglycsedg
      361 vvsmrvsdgp peacsdeeaa spaetavslk gtgiqecavt lvppaadpvn fgkvgmieda
      421 tsallekgqq qiafdddkge pcgasscmas vedscghknc aamslpkkle dastgctaet
      481 vfdgalaapk qfegsliqkd vpgdqqgcte tlkalsvegd rqrlvvhvds gsdsdfnafq
      541 fwrmpipele vdieflgnrp tavhvraksq sqgnrcfssd lsvevlgdng kpintsgdld
      601 lnqdgaissq rlsgdstlri rtaslsslts adqecnills ssvteasvaf neagevagvh
      661 kvrmsnskev agtentllak slntssdsws aheqdivpvl lleryqsmtd ptlaqnldsd
      721 igrhcahslp avaltlgrqf wpclrttyrc lvsdvqwkvr hivasclhel atilgpeias
      781 qdlvpsfegf vkdvdevrvg llerlalflr glskadqrrl lplladflcm dnqrswrfrf
      841 tlarqlkeia alyspldvhq ylvpmvsell vdkvaevrhi sveamvavlq qlaphylmck
      901 ellqqlceka qdrkwcqrql fcelaeqmis aeclsgeefi gtvlpkllcl rhdsvpnvrm
      961 alarclvrgv wpkaffwdsg niykdelkat laslqedtdr dvrfyaqlyp kdsvd
//
LOCUS       XP_077504043            1005 aa            linear   INV 25-NOV-2025
DEFINITION  serine/threonine-protein phosphatase 4 regulatory subunit 1-like
            isoform X4 [Amblyomma americanum].
ACCESSION   XP_077504043
VERSION     XP_077504043.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647917.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1005
                     /product="serine/threonine-protein phosphatase 4
                     regulatory subunit 1-like isoform X4"
                     /calculated_mol_wt=110512
     Region          99..131
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          149..177
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(165,170,173,176..177,207..208,211,214..215,218,
                     246..247,250,253..254,257,284..285,288,291..292,323..324,
                     327,330)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          189..218
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          228..258
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          267..292
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          303..330
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          524..>548
                     /region_name="WRNPLPNID"
                     /note="Putative WW-binding domain and destruction box;
                     pfam15017"
                     /db_xref="CDD:464448"
     Site            order(751..752,755,758..759,830..831,834,837..838,841,
                     869..870,873,876..877,880,916..917,920,950..951,954)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          773..841
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          776..801
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          851..881
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          891..921
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          930..956
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..1005
                     /gene="LOC144114287"
                     /coded_by="XM_077647917.1:53..3070"
                     /db_xref="GeneID:144114287"
ORIGIN      
        1 magtsgeads vnpgneassc wsnfslamlt adqeetsaeq igegceddda asrrqellee
       61 pnpvvklery aysdivfnrq lvarnivdtl ravvdsqeav davltalssl sldcepsvrt
      121 elveqlpnia sfcaseggll qnvvrehvvp ilvqyltdva nqvrkrtqvs llfilerali
      181 drkqleeqic slvvhltese nvdeyrmeav tlmtkmapyv gkdttaylfl prfislcqdd
      241 sfhvrkvcaa nfgdfssivg qkytednipv fqmlcedcvw gvrkacaevf mpvscicsla
      301 trrnrltplf gsllddqsrw vrsaayqglg pfistfadpl rtglycsedg vvsmrvsdgp
      361 peacsdeeaa spaetavslk gtgiqecavt lvppaadpvn fgkvgmieda tsallekgqq
      421 qiafdddkge pcgasscmas vedscghknc aamslpkkle dastgctaet vfdgalaapk
      481 qfegsliqkd vpgdqqgcte tlkalsvegd rqrlvvhvds gsdsdfnafq fwrmpipele
      541 vdieflgnrp tavhvraksq sqgnrcfssd lsvevlgdng kpintsgdld lnqdgaissq
      601 rlsgdstlri rtaslsslts adqecnills ssvteasvaf neagevagvh kvrmsnskev
      661 agtentllak slntssdsws aheqdivpvl lleryqsmtd ptlaqnldsd igrhcahslp
      721 avaltlgrqf wpclrttyrc lvsdvqwkvr hivasclhel atilgpeias qdlvpsfegf
      781 vkdvdevrvg llerlalflr glskadqrrl lplladflcm dnqrswrfrf tlarqlkeia
      841 alyspldvhq ylvpmvsell vdkvaevrhi sveamvavlq qlaphylmck ellqqlceka
      901 qdrkwcqrql fcelaeqmis aeclsgeefi gtvlpkllcl rhdsvpnvrm alarclvrgv
      961 wpkaffwdsg niykdelkat laslqedtdr dvrfyaqlyp kdsvd
//
LOCUS       XP_077504044             928 aa            linear   INV 25-NOV-2025
DEFINITION  serine/threonine-protein phosphatase 4 regulatory subunit 1-like
            isoform X5 [Amblyomma americanum].
ACCESSION   XP_077504044
VERSION     XP_077504044.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647918.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..928
                     /product="serine/threonine-protein phosphatase 4
                     regulatory subunit 1-like isoform X5"
                     /calculated_mol_wt=102114
     Region          26..54
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          72..100
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(88,93,96,99..100,130..131,134,137..138,141,169..170,
                     173,176..177,180,207..208,211,214..215,246..247,250,253)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          112..141
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          151..181
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          190..215
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          226..253
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          447..>471
                     /region_name="WRNPLPNID"
                     /note="Putative WW-binding domain and destruction box;
                     pfam15017"
                     /db_xref="CDD:464448"
     Site            order(674..675,678,681..682,753..754,757,760..761,764,
                     792..793,796,799..800,803,839..840,843,873..874,877)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          696..764
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          699..724
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          774..804
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          814..844
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          853..879
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..928
                     /gene="LOC144114287"
                     /coded_by="XM_077647918.1:533..3319"
                     /db_xref="GeneID:144114287"
ORIGIN      
        1 mgqlvarniv dtlravvdsq eavdavltal sslsldceps vrtelveqlp niasfcaseg
       61 gllqnvvreh vvpilvqylt dvanqvrkrt qvsllfiler alidrkqlee qicslvvhlt
      121 esenvdeyrm eavtlmtkma pyvgkdttay lflprfislc qddsfhvrkv caanfgdfss
      181 ivgqkytedn ipvfqmlced cvwgvrkaca evfmpvscic slatrrnrlt plfgsllddq
      241 srwvrsaayq glgpfistfa dplrtglycs edgvvsmrvs dgppeacsde eaaspaetav
      301 slkgtgiqec avtlvppaad pvnfgkvgmi edatsallek gqqqiafddd kgepcgassc
      361 masvedscgh kncaamslpk kledastgct aetvfdgala apkqfegsli qkdvpgdqqg
      421 ctetlkalsv egdrqrlvvh vdsgsdsdfn afqfwrmpip elevdieflg nrptavhvra
      481 ksqsqgnrcf ssdlsvevlg dngkpintsg dldlnqdgai ssqrlsgdst lrirtaslss
      541 ltsadqecni llsssvteas vafneageva gvhkvrmsns kevagtentl lakslntssd
      601 swsaheqdiv pvllleryqs mtdptlaqnl dsdigrhcah slpavaltlg rqfwpclrtt
      661 yrclvsdvqw kvrhivascl helatilgpe iasqdlvpsf egfvkdvdev rvgllerlal
      721 flrglskadq rrllplladf lcmdnqrswr frftlarqlk eiaalyspld vhqylvpmvs
      781 ellvdkvaev rhisveamva vlqqlaphyl mckellqqlc ekaqdrkwcq rqlfcelaeq
      841 misaeclsge efigtvlpkl lclrhdsvpn vrmalarclv rgvwpkaffw dsgniykdel
      901 katlaslqed tdrdvrfyaq lypkdsvd
//
LOCUS       XP_077504046             116 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114289 [Amblyomma americanum].
ACCESSION   XP_077504046
VERSION     XP_077504046.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647920.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..116
                     /product="uncharacterized protein LOC144114289"
                     /calculated_mol_wt=12869
     CDS             1..116
                     /gene="LOC144114289"
                     /coded_by="XM_077647920.1:514..864"
                     /db_xref="GeneID:144114289"
ORIGIN      
        1 mttaqaisdh ervltgkpng htllansman ppprsvyrwh qewrsqkygp qgnsslklqe
       61 nkprylaqgt dvsitsvadd swavlvvtpi mhraqqlesa rdiifvdsts iimqln
//
LOCUS       XP_077504047             318 aa            linear   INV 25-NOV-2025
DEFINITION  BTB/POZ domain-containing protein 1-like [Amblyomma americanum].
ACCESSION   XP_077504047
VERSION     XP_077504047.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647921.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..318
                     /product="BTB/POZ domain-containing protein 1-like"
                     /calculated_mol_wt=36119
     Region          50..165
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     CDS             1..318
                     /gene="LOC144114291"
                     /coded_by="XM_077647921.1:136..1092"
                     /db_xref="GeneID:144114291"
ORIGIN      
        1 meghqettss frlrsranrv gpywrnganr gygsrsdvst qteeqrefsl arfleseema
       61 dveiivncah fpnqrksyka hriilglqnk iframlsggf rgvdriiitd lhpegvhall
      121 ryfyngqlev etlhqalytr saatkylvpe leemciayik rnmkpqdvcp vfdylftmge
      181 dhsdlpaldl lkeysltvlt seafarcsvr tanyviecva dvpqisvfra lhrwaqeqrl
      241 rspaavdgkp evrtlvkpff pklhflalta eefvtgpnlw gilndheara vlsnivkkgs
      301 lplppgfsny dyssseek
//
LOCUS       XP_077504048            1863 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114292 [Amblyomma americanum].
ACCESSION   XP_077504048
VERSION     XP_077504048.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647922.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1863
                     /product="uncharacterized protein LOC144114292"
                     /calculated_mol_wt=198857
     Region          <6..>194
                     /region_name="dermokine"
                     /note="cl42387"
                     /db_xref="CDD:455732"
     Region          <218..344
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     Region          <1121..1646
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          <1427..1654
                     /region_name="PTZ00449"
                     /note="104 kDa microneme/rhoptry antigen; Provisional"
                     /db_xref="CDD:185628"
     Region          <1822..>1862
                     /region_name="Formin_GBD_N"
                     /note="Formin N-terminal GTPase-binding domain; pfam18382"
                     /db_xref="CDD:465735"
     CDS             1..1863
                     /gene="LOC144114292"
                     /coded_by="XM_077647922.1:510..6101"
                     /db_xref="GeneID:144114292"
ORIGIN      
        1 msrrtgsyrs ggsaptrygg sgnysgncts prsstygsps ygssgrysgg lapslsiysp
       61 sykasgsgyg sdygrssrps sgygsgygsg ygsdygkgag agyasdygrs ggygsdygsr
      121 nsgyasdynr sstlprpgts ygrqschqga pgapvagnts ystyrrpssg gnsvanavkm
      181 fgslalrdda sskppvsrsy svkakedaar ppshgmpsre esksfvnvra kadeeqpdaa
      241 eakatdstpk nlaksdsksg krglapadft stsdsesdde dqsgggegnq adgggavsrg
      301 tstpfdgagd saaisntdra pprppaskqq aavrrsagqq vdplelespr yggskrptsf
      361 nsaiprqisr rassspassa rsrsstslst atpparalfl tnscsripvl ngstvkktps
      421 ppraaalssp tstsceeece teqnkdfrks vlnmnlteee rekylsrqre erercsqhgd
      481 teykmdssna teatttttet etsedegavv lsksasstvg rsgsksllle pglrpsgskv
      541 ivsrsssqnv ltkrgsrdni lqssqsndri ydrsrsasga dlleattttd ddedasears
      601 rrvrrrrkgs kenildggtd kpaptaaapv kydrqestss vassstssse ptdrpqmgap
      661 hvtvsthtts egddeeeddd ededednede vaslavtpvp sgdhlspeps paapikdetl
      721 aaeqdvnqes eesqedeeee deeektldfs gdekvatggl sdneisdaad etaavardre
      781 vdsagneetp eseqksdaet aacpteipee ktpkffsese kseeegeedd eeddeeeeqe
      841 dekelkheee dfehkeveei klqaeeassa edeaeneimh keidvdateh saeadggkes
      901 eeegasdrss segpcdkeks peiiigkese aevseesdkd eqsdeeldda gakddggddd
      961 gegkerppsp lcepaedaee fvkdgtkaee tsdeteeetd ddshgqkndv sasladereq
     1021 ktpvltegse kveeevetdd hkenqatqdv ieatstesvh yvesktdsfp ntasprtfpd
     1081 ilrirdaspv ltsdasarsn ksetcivsmh spenrqeiss psslakpias epcqlaktps
     1141 sqsvtsptil spvvtnrtal pyvkqplpge tpteeklgsp aaaplspfsh psvssvnria
     1201 sqfssptpvp qsppvrrtrp thsgiptpsv tndskpdenk slsaaqpkpq lgvtrrqrpt
     1261 vsssvqpsqq ssiptsssts atqasvpspr vrpvpvppat gvsstrvrsv pvapppapvs
     1321 lvrvfpvppp petpaaaptv ssaktqppev lpvaqekisl asparpafat aqekapppat
     1381 parpeaqasp rflssqekth apaapikpaa qtspafqatq ekmhavstpt kpaaqtpmpf
     1441 tprqektqps ppaprtitlp ttaqrptsld igkentnras psadsflqny rrsrdavsks
     1501 dssghiksps vlaaqrtfls gdakqittpe kapvevpkap fttpskppve vtkasftspa
     1561 kppaevpkas ftspakpsve vpkasfttpa kppceppkgk fqktpeqtrt vfgipppgrr
     1621 piaaakpvea kkspqevrpq pvptrpndqv kngrkpvtkf igsckdidsl lkygsdeepe
     1681 tfeqfedqfe kgaaiklplp kelkkhspgd rvkhfigkfq diddmlgapt atpvfplspc
     1741 drspfersft dsqarktqqd dsdssgdssl eevkvssvkv heskqairpr ldayteeids
     1801 savrirepkv mqgqitkrpt rklddctlql ykinaadndy gsyldlesti deqgddfegf
     1861 qdk
//
LOCUS       XP_077504049            1009 aa            linear   INV 25-NOV-2025
DEFINITION  CUB and LDLa domain isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504049
VERSION     XP_077504049.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647923.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1009
                     /product="CUB and LDLa domain isoform X1"
                     /calculated_mol_wt=109626
     Region          239..335
                     /region_name="CUB"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; cd00041"
                     /db_xref="CDD:238001"
     Site            order(239,241,243,271,276,305,330,332,334..335)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     Region          478..511
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(482,490,501..502)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(494,497,501,507..508)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            504..508
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          641..>995
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..1009
                     /gene="Culd"
                     /coded_by="XM_077647923.1:464..3493"
                     /db_xref="GeneID:144114293"
ORIGIN      
        1 maaarpeaaa rrrrtrpptw rpepsgrram hrqqrqrtsw rggvgrscgv akdrrllwkg
       61 wwvllmavaf lgtpaaakpd gdlcrwmngr kyyvalgdtg sitatnvttd gggansngdh
      121 dpnqpripqr csvelitcps chivvsllyi nipscssdgs cqcdyvwvke ptvgksgqef
      181 cgirsnqtvq kyesktklls idflysfsya dafsiqftsl enkyklegqv dyqhkggasg
      241 fvesphfpvg ypsdysseht mqnldergfv qlvftdfqls pwsyvevldn gsrvdvyngn
      301 tfrppivasa gptlvvrfra ndeypdlgfr akytfvnype kywfnkpstd cggvvedlgg
      361 aitmmnmanp gytrnfdciw llrpstsyty ethisvrvaq feemgpessl eirqgltsvs
      421 flleslaghq shktpqgqey iisanvgfyi rlqgsfsnks rlaivysifs yetlqcqssg
      481 dfecgngrci ksilhcdgfn hcgdgsdesa cfarnkssaa avpgsgghlp prdsnwwrsl
      541 tpnyyfpkqe ssaaagtntl ilitslaglg mfvlttvmil vklhkqrrle vvgrdalrti
      601 sadvdsdags vgqgrhpfgl ngtplydppp syedvikfym ppppaysaiv earsavpvss
      661 npnpshhsas asapshrlgr gienaayvpd elqcpsrqgs gassdrrrss ssskathllr
      721 vilppvtvfe rapqrrhkst svdpkqlglr cepssrgqps rhrhgavpav hssnssggss
      781 sssgsssgss sllassglvh tlldrraptt saepncslss pardqaspks lsqarcqcag
      841 acscatpsrt kvrrmlhtaa kyqhlndgsg nipasgless erspekvtar kvpakscsda
      901 lmpepstkaw vepllesikp aqsrgprhpt ftssasenps tdlqatemtv tkdtqpllrr
      961 tsslpelaav lparlrvmph eaipapgpns aapcdqvdsp kmimestqq
//
LOCUS       XP_077504050            1001 aa            linear   INV 25-NOV-2025
DEFINITION  CUB and LDLa domain isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504050
VERSION     XP_077504050.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647924.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1001
                     /product="CUB and LDLa domain isoform X2"
                     /calculated_mol_wt=108840
     Region          239..335
                     /region_name="CUB"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; cd00041"
                     /db_xref="CDD:238001"
     Site            order(239,241,243,271,276,305,330,332,334..335)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     Region          478..511
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(482,490,501..502)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(494,497,501,507..508)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            504..508
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          633..>987
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..1001
                     /gene="Culd"
                     /coded_by="XM_077647924.1:464..3469"
                     /db_xref="GeneID:144114293"
ORIGIN      
        1 maaarpeaaa rrrrtrpptw rpepsgrram hrqqrqrtsw rggvgrscgv akdrrllwkg
       61 wwvllmavaf lgtpaaakpd gdlcrwmngr kyyvalgdtg sitatnvttd gggansngdh
      121 dpnqpripqr csvelitcps chivvsllyi nipscssdgs cqcdyvwvke ptvgksgqef
      181 cgirsnqtvq kyesktklls idflysfsya dafsiqftsl enkyklegqv dyqhkggasg
      241 fvesphfpvg ypsdysseht mqnldergfv qlvftdfqls pwsyvevldn gsrvdvyngn
      301 tfrppivasa gptlvvrfra ndeypdlgfr akytfvnype kywfnkpstd cggvvedlgg
      361 aitmmnmanp gytrnfdciw llrpstsyty ethisvrvaq feemgpessl eirqgltsvs
      421 flleslaghq shktpqgqey iisanvgfyi rlqgsfsnks rlaivysifs yetlqcqssg
      481 dfecgngrci ksilhcdgfn hcgdgsdesa cfavpgsggh lpprdsnwwr sltpnyyfpk
      541 qessaaagtn tlilitslag lgmfvlttvm ilvklhkqrr levvgrdalr tisadvdsda
      601 gsvgqgrhpf glngtplydp ppsyedvikf ymppppaysa ivearsavpv ssnpnpshhs
      661 asasapshrl grgienaayv pdelqcpsrq gsgassdrrr ssssskathl lrvilppvtv
      721 ferapqrrhk stsvdpkqlg lrcepssrgq psrhrhgavp avhssnssgg sssssgsssg
      781 sssllassgl vhtlldrrap ttsaepncsl sspardqasp kslsqarcqc agacscatps
      841 rtkvrrmlht aakyqhlndg sgnipasgle sserspekvt arkvpakscs dalmpepstk
      901 awvepllesi kpaqsrgprh ptftssasen pstdlqatem tvtkdtqpll rrtsslpela
      961 avlparlrvm pheaipapgp nsaapcdqvd spkmimestq q
//
LOCUS       XP_077504051             900 aa            linear   INV 25-NOV-2025
DEFINITION  pyruvate dehydrogenase phosphatase regulatory subunit,
            mitochondrial isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504051
VERSION     XP_077504051.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647925.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..900
                     /product="pyruvate dehydrogenase phosphatase regulatory
                     subunit, mitochondrial isoform X1"
                     /calculated_mol_wt=100853
     Region          64..427
                     /region_name="DadA"
                     /note="Glycine/D-amino acid oxidase (deaminating) [Amino
                     acid transport and metabolism]; COG0665"
                     /db_xref="CDD:440429"
     Region          413..468
                     /region_name="FAO_M"
                     /note="FAD dependent oxidoreductase central domain;
                     pfam16350"
                     /db_xref="CDD:465100"
     Region          462..877
                     /region_name="GcvT"
                     /note="Glycine cleavage system protein T
                     (aminomethyltransferase) [Amino acid transport and
                     metabolism]; COG0404"
                     /db_xref="CDD:440173"
     CDS             1..900
                     /gene="LOC144114295"
                     /coded_by="XM_077647925.1:196..2898"
                     /db_xref="GeneID:144114295"
ORIGIN      
        1 mapvrsprlp lrlidlyryg qystlsnlrl anagqavekt ppgpldlpse acvvicgggv
       61 vgcsvayhla rdhgltdvvv lekgvigggs tkhgagllgq lkattiecrv skysiqlykd
      121 fesrgfdtgw nqcgslllar tkdrlfyfkr laamatslnl echllspaea qlkapeitte
      181 llegalwvpg dgvadpslvc kvfaqqasem gikimegcsv ekvtsrgqhn vhdvetnwgt
      241 irceyfvnsa gyfgrhvgkm stpevkvplh pcghhflhtl pveginpvmp vihdcdghfy
      301 lrahegayla ggfepkskpa dlsilpsrel gtlpedwdqf yillkemlfr fpnlgnievd
      361 rlyncpesft pdcrwiigea peaknyfvas gmksvgieaa gglgevtaew iakgepkldl
      421 wdidirrfig ihnnahflrd rmteapgmhy rlcypfvefq tgrelrmspi yprlklagav
      481 fgqtmgyerp hyytssdpni ltgmdlpsak vemygkppwf davkseyeac rervalldys
      541 sftklevwsp gsevvdllqy lcsndinipv ggivntgmqn hwggyendcs vvrleknrnr
      601 vsrdtsscdl vhrfmivapt kqqmrcqtwi rrhvpydspi vvadvtsmyt aicimgqrap
      661 nlmseltdts ltskdfplft wkeldiglas gikvfhmtht gdrgwvlyip neyalhvydr
      721 ilevgqkygi qhagyfamrt lriekffafw gkdldctttp fecgrafrvk fdkdflgkea
      781 llkqkeagvt rryvqlllke hdpdydpwpw ggepilfedr cigmtsttcy gftlggqvcl
      841 gfvenkdeng vaqvltndfi lknefyvdia ghkfaarani hspslayqvt tpdrlylatr
//
LOCUS       XP_077504052             885 aa            linear   INV 25-NOV-2025
DEFINITION  pyruvate dehydrogenase phosphatase regulatory subunit,
            mitochondrial isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504052
VERSION     XP_077504052.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647926.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..885
                     /product="pyruvate dehydrogenase phosphatase regulatory
                     subunit, mitochondrial isoform X2"
                     /calculated_mol_wt=99126
     Region          64..427
                     /region_name="DadA"
                     /note="Glycine/D-amino acid oxidase (deaminating) [Amino
                     acid transport and metabolism]; COG0665"
                     /db_xref="CDD:440429"
     Region          413..468
                     /region_name="FAO_M"
                     /note="FAD dependent oxidoreductase central domain;
                     pfam16350"
                     /db_xref="CDD:465100"
     Region          462..862
                     /region_name="GcvT"
                     /note="Glycine cleavage system protein T
                     (aminomethyltransferase) [Amino acid transport and
                     metabolism]; COG0404"
                     /db_xref="CDD:440173"
     CDS             1..885
                     /gene="LOC144114295"
                     /coded_by="XM_077647926.1:196..2853"
                     /db_xref="GeneID:144114295"
ORIGIN      
        1 mapvrsprlp lrlidlyryg qystlsnlrl anagqavekt ppgpldlpse acvvicgggv
       61 vgcsvayhla rdhgltdvvv lekgvigggs tkhgagllgq lkattiecrv skysiqlykd
      121 fesrgfdtgw nqcgslllar tkdrlfyfkr laamatslnl echllspaea qlkapeitte
      181 llegalwvpg dgvadpslvc kvfaqqasem gikimegcsv ekvtsrgqhn vhdvetnwgt
      241 irceyfvnsa gyfgrhvgkm stpevkvplh pcghhflhtl pveginpvmp vihdcdghfy
      301 lrahegayla ggfepkskpa dlsilpsrel gtlpedwdqf yillkemlfr fpnlgnievd
      361 rlyncpesft pdcrwiigea peaknyfvas gmksvgieaa gglgevtaew iakgepkldl
      421 wdidirrfig ihnnahflrd rmteapgmhy rlcypfvefq tgrelrmspi yprlklagav
      481 fgqtmgyerp hyytssdpni ltgmdlpsak vemygkppwf davkseyeac rervalldys
      541 sftklevwsp gsevvdllqy lcsndinipv ggivntgmqn hwggyendcs vvrleknrfm
      601 ivaptkqqmr cqtwirrhvp ydspivvadv tsmytaicim gqrapnlmse ltdtsltskd
      661 fplftwkeld iglasgikvf hmthtgdrgw vlyipneyal hvydrilevg qkygiqhagy
      721 famrtlriek ffafwgkdld ctttpfecgr afrvkfdkdf lgkeallkqk eagvtrryvq
      781 lllkehdpdy dpwpwggepi lfedrcigmt sttcygftlg gqvclgfven kdengvaqvl
      841 tndfilknef yvdiaghkfa aranihspsl ayqvttpdrl ylatr
//
LOCUS       XP_077504053             732 aa            linear   INV 25-NOV-2025
DEFINITION  pyruvate dehydrogenase phosphatase regulatory subunit,
            mitochondrial isoform X3 [Amblyomma americanum].
ACCESSION   XP_077504053
VERSION     XP_077504053.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647927.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..732
                     /product="pyruvate dehydrogenase phosphatase regulatory
                     subunit, mitochondrial isoform X3"
                     /calculated_mol_wt=82602
     Region          <1..274
                     /region_name="DadA"
                     /note="Glycine/D-amino acid oxidase (deaminating) [Amino
                     acid transport and metabolism]; COG0665"
                     /db_xref="CDD:440429"
     Region          260..315
                     /region_name="FAO_M"
                     /note="FAD dependent oxidoreductase central domain;
                     pfam16350"
                     /db_xref="CDD:465100"
     Region          309..709
                     /region_name="GcvT"
                     /note="Glycine cleavage system protein T
                     (aminomethyltransferase) [Amino acid transport and
                     metabolism]; COG0404"
                     /db_xref="CDD:440173"
     CDS             1..732
                     /gene="LOC144114295"
                     /coded_by="XM_077647927.1:529..2727"
                     /db_xref="GeneID:144114295"
ORIGIN      
        1 matslnlech llspaeaqlk apeittelle galwvpgdgv adpslvckvf aqqasemgik
       61 imegcsvekv tsrgqhnvhd vetnwgtirc eyfvnsagyf grhvgkmstp evkvplhpcg
      121 hhflhtlpve ginpvmpvih dcdghfylra hegaylaggf epkskpadls ilpsrelgtl
      181 pedwdqfyil lkemlfrfpn lgnievdrly ncpesftpdc rwiigeapea knyfvasgmk
      241 svgieaaggl gevtaewiak gepkldlwdi dirrfigihn nahflrdrmt eapgmhyrlc
      301 ypfvefqtgr elrmspiypr lklagavfgq tmgyerphyy tssdpniltg mdlpsakvem
      361 ygkppwfdav kseyeacrer valldyssft klevwspgse vvdllqylcs ndinipvggi
      421 vntgmqnhwg gyendcsvvr leknrfmiva ptkqqmrcqt wirrhvpyds pivvadvtsm
      481 ytaicimgqr apnlmseltd tsltskdfpl ftwkeldigl asgikvfhmt htgdrgwvly
      541 ipneyalhvy drilevgqky giqhagyfam rtlriekffa fwgkdldctt tpfecgrafr
      601 vkfdkdflgk eallkqkeag vtrryvqlll kehdpdydpw pwggepilfe drcigmtstt
      661 cygftlggqv clgfvenkde ngvaqvltnd filknefyvd iaghkfaara nihspslayq
      721 vttpdrlyla tr
//
LOCUS       XP_077504054             732 aa            linear   INV 25-NOV-2025
DEFINITION  pyruvate dehydrogenase phosphatase regulatory subunit,
            mitochondrial isoform X3 [Amblyomma americanum].
ACCESSION   XP_077504054
VERSION     XP_077504054.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647928.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..732
                     /product="pyruvate dehydrogenase phosphatase regulatory
                     subunit, mitochondrial isoform X3"
                     /calculated_mol_wt=82602
     Region          <1..274
                     /region_name="DadA"
                     /note="Glycine/D-amino acid oxidase (deaminating) [Amino
                     acid transport and metabolism]; COG0665"
                     /db_xref="CDD:440429"
     Region          260..315
                     /region_name="FAO_M"
                     /note="FAD dependent oxidoreductase central domain;
                     pfam16350"
                     /db_xref="CDD:465100"
     Region          309..709
                     /region_name="GcvT"
                     /note="Glycine cleavage system protein T
                     (aminomethyltransferase) [Amino acid transport and
                     metabolism]; COG0404"
                     /db_xref="CDD:440173"
     CDS             1..732
                     /gene="LOC144114295"
                     /coded_by="XM_077647928.1:384..2582"
                     /db_xref="GeneID:144114295"
ORIGIN      
        1 matslnlech llspaeaqlk apeittelle galwvpgdgv adpslvckvf aqqasemgik
       61 imegcsvekv tsrgqhnvhd vetnwgtirc eyfvnsagyf grhvgkmstp evkvplhpcg
      121 hhflhtlpve ginpvmpvih dcdghfylra hegaylaggf epkskpadls ilpsrelgtl
      181 pedwdqfyil lkemlfrfpn lgnievdrly ncpesftpdc rwiigeapea knyfvasgmk
      241 svgieaaggl gevtaewiak gepkldlwdi dirrfigihn nahflrdrmt eapgmhyrlc
      301 ypfvefqtgr elrmspiypr lklagavfgq tmgyerphyy tssdpniltg mdlpsakvem
      361 ygkppwfdav kseyeacrer valldyssft klevwspgse vvdllqylcs ndinipvggi
      421 vntgmqnhwg gyendcsvvr leknrfmiva ptkqqmrcqt wirrhvpyds pivvadvtsm
      481 ytaicimgqr apnlmseltd tsltskdfpl ftwkeldigl asgikvfhmt htgdrgwvly
      541 ipneyalhvy drilevgqky giqhagyfam rtlriekffa fwgkdldctt tpfecgrafr
      601 vkfdkdflgk eallkqkeag vtrryvqlll kehdpdydpw pwggepilfe drcigmtstt
      661 cygftlggqv clgfvenkde ngvaqvltnd filknefyvd iaghkfaara nihspslayq
      721 vttpdrlyla tr
//
LOCUS       XP_077504055             722 aa            linear   INV 25-NOV-2025
DEFINITION  pyruvate dehydrogenase phosphatase regulatory subunit,
            mitochondrial isoform X4 [Amblyomma americanum].
ACCESSION   XP_077504055
VERSION     XP_077504055.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647929.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..722
                     /product="pyruvate dehydrogenase phosphatase regulatory
                     subunit, mitochondrial isoform X4"
                     /calculated_mol_wt=81601
     Region          <5..249
                     /region_name="DadA"
                     /note="Glycine/D-amino acid oxidase (deaminating) [Amino
                     acid transport and metabolism]; COG0665"
                     /db_xref="CDD:440429"
     Region          235..290
                     /region_name="FAO_M"
                     /note="FAD dependent oxidoreductase central domain;
                     pfam16350"
                     /db_xref="CDD:465100"
     Region          284..699
                     /region_name="GcvT"
                     /note="Glycine cleavage system protein T
                     (aminomethyltransferase) [Amino acid transport and
                     metabolism]; COG0404"
                     /db_xref="CDD:440173"
     CDS             1..722
                     /gene="LOC144114295"
                     /coded_by="XM_077647929.1:84..2252"
                     /db_xref="GeneID:144114295"
ORIGIN      
        1 msssqgalwv pgdgvadpsl vckvfaqqas emgikimegc svekvtsrgq hnvhdvetnw
       61 gtirceyfvn sagyfgrhvg kmstpevkvp lhpcghhflh tlpveginpv mpvihdcdgh
      121 fylrahegay laggfepksk padlsilpsr elgtlpedwd qfyillkeml frfpnlgnie
      181 vdrlyncpes ftpdcrwiig eapeaknyfv asgmksvgie aagglgevta ewiakgepkl
      241 dlwdidirrf igihnnahfl rdrmteapgm hyrlcypfve fqtgrelrms piyprlklag
      301 avfgqtmgye rphyytssdp niltgmdlps akvemygkpp wfdavkseye acrervalld
      361 yssftklevw spgsevvdll qylcsndini pvggivntgm qnhwggyend csvvrleknr
      421 nrvsrdtssc dlvhrfmiva ptkqqmrcqt wirrhvpyds pivvadvtsm ytaicimgqr
      481 apnlmseltd tsltskdfpl ftwkeldigl asgikvfhmt htgdrgwvly ipneyalhvy
      541 drilevgqky giqhagyfam rtlriekffa fwgkdldctt tpfecgrafr vkfdkdflgk
      601 eallkqkeag vtrryvqlll kehdpdydpw pwggepilfe drcigmtstt cygftlggqv
      661 clgfvenkde ngvaqvltnd filknefyvd iaghkfaara nihspslayq vttpdrlyla
      721 tr
//
LOCUS       XP_077504057            1001 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114296 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504057
VERSION     XP_077504057.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647931.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1001
                     /product="uncharacterized protein LOC144114296 isoform X1"
                     /calculated_mol_wt=112841
     Region          58..134
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          339..>481
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..1001
                     /gene="LOC144114296"
                     /coded_by="XM_077647931.1:138..3143"
                     /db_xref="GeneID:144114296"
ORIGIN      
        1 mtqrvcaasa aagnaaaqwq qttatgasip klrerlptrr ahwtsvitvl svmshmycca
       61 kwcstsgksg ehlfrfpsdh rfsiwlkyan rlelletpre kvyktyrlcs khftrdcfts
      121 dactrlthea vpsvkvhepr lralvhpdfy eegaaqgies asplqstaap vevmlghdyl
      181 plpstlglgt iagadsavhd tddphssrap qltdvqttpp ppadeegaaq stpirviasa
      241 swdspdyaea gpsglsgake svrqpvmtpa sgtpksrrrk gavvytprtk krfeklhsrs
      301 dryrkalsri rkrhdtrrvt qqealnklep hlpqdlfelv kaqirlcsfp kskkhwskqm
      361 kqfalnlffh spkayrflre mlhlphvrtl rrwladipmt pgiipgamda iesitkdwpl
      421 qdkaccllfd eislkknlmy dqskdivigf tddgntrtsr vanialvmav sgisrswmqp
      481 vafaashnat svlamrklll dliqqlqtkg llvkavicdq gsnnvslsrm lihsihepyf
      541 vvnnrkiyfm fdtphlikct rnnlrrhklt igsevvdwth ietlykktrh idrasktpve
      601 hlksrlakkl keqhiyrmpf admrvkyatq vfsesvsval ltliaiqelp vdakftaevt
      661 ermdklfdcl nssalkkqdd klryamtegs ehhvflesci dwiaqwhfgg pldkqphtik
      721 gwqitikall llwtdlkadf dythlftrrl qqdplenffa ivrqkhgcne tpnvyqfvaa
      781 vkhiwigqlf klsqgncevt thafltnles vsavlspsst pstshsrqqg sgssdlqass
      841 qdapdivyen vvyhvagtlv ksfldqrtte ctcegtllaa dggslhgrhq ffallkengv
      901 pehlfqsiaa tseaclnyik kldsafrkei sccahlsnvs rnlvermpnq qavfcslgck
      961 ikflklfcrt rlhwhlsfln rsmtsktkqs aparkrrkla g
//
LOCUS       XP_077504058             231 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114296 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504058
VERSION     XP_077504058.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647932.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..231
                     /product="uncharacterized protein LOC144114296 isoform X2"
                     /calculated_mol_wt=25330
     Region          58..134
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..231
                     /gene="LOC144114296"
                     /coded_by="XM_077647932.1:156..851"
                     /db_xref="GeneID:144114296"
ORIGIN      
        1 mtqrvcaasa aagnaaaqwq qttatgasip klrerlptrr ahwtsvitvl svmshmycca
       61 kwcstsgksg ehlfrfpsdh rfsiwlkyan rlelletpre kvyktyrlcs khftrdcfts
      121 dactrlthea vpsvkvhepr lralvhpdfy eegaaqgies asplqstaap vevmlghdyl
      181 plpstlglgt iagadsavhd tddphssrap qltdvqttpp ppadeevclf i
//
LOCUS       XP_077504059             430 aa            linear   INV 25-NOV-2025
DEFINITION  elongation factor 1-gamma [Amblyomma americanum].
ACCESSION   XP_077504059
VERSION     XP_077504059.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647933.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..430
                     /product="elongation factor 1-gamma"
                     /calculated_mol_wt=48974
     Region          4..82
                     /region_name="GST_N_EF1Bgamma"
                     /note="GST_N family, Gamma subunit of Elongation Factor 1B
                     (EFB1gamma) subfamily; EF1Bgamma is part of the eukaryotic
                     translation elongation factor-1 (EF1) complex which plays
                     a central role in the elongation cycle during protein
                     biosynthesis. EF1 consists of...; cd03044"
                     /db_xref="CDD:239342"
     Site            order(13,16,19..20,23,73)
                     /site_type="other"
                     /note="C-terminal domain interface [polypeptide binding]"
                     /db_xref="CDD:239342"
     Site            order(14,57..59,71..72)
                     /site_type="other"
                     /note="putative GSH binding site (G-site) [chemical
                     binding]"
                     /db_xref="CDD:239342"
     Site            order(57,70..71,73..74,77)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:239342"
     Region          91..213
                     /region_name="GST_C_EF1Bgamma_like"
                     /note="Glutathione S-transferase C-terminal-like, alpha
                     helical domain of the Gamma subunit of Elongation Factor
                     1B and similar proteins; cd03181"
                     /db_xref="CDD:198290"
     Site            order(96..97,100..101,104,141)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:198290"
     Site            order(103,166,170,199,202..204)
                     /site_type="other"
                     /note="N-terminal domain interface [polypeptide binding]"
                     /db_xref="CDD:198290"
     Region          273..376
                     /region_name="EF1G"
                     /note="Elongation factor 1 gamma, conserved domain;
                     pfam00647"
                     /db_xref="CDD:459888"
     CDS             1..430
                     /gene="eEF1gamma"
                     /coded_by="XM_077647933.1:32..1324"
                     /db_xref="GeneID:144114297"
ORIGIN      
        1 matgtlytwk dnfrahkili aakysqfklk vcceapdfvf gdtncsqsfl ekfplgkvpa
       61 fetsdgqait esnaiafyvs ndqlrgknll eqaqviqflt fadceilppa ctwvfpclga
      121 iqynkqater akedvkkils ylnahlllkt ylvgeritla diavftallp lyklvlepaf
      181 rgpysnvnrw fdtlahqpef qavlgevelc qkmaqfdaal yaevqgkagk sagaskkaek
      241 kdakpkdepk skaasapepd ddlepalaep pkkdpfeafp kgtfnmddfk rcysnesetv
      301 svpyfwekfd kehysiwfce ykypeeltqv fmscnlisgm fqrldkmrkh afasvilfge
      361 dnnssisgiw vwrghelafk lsedwqvdye sydwkkldad tpetkklvde yfkwegdfsg
      421 kkfnqgkifk
//
LOCUS       XP_077504060             207 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114300 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504060
VERSION     XP_077504060.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647934.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..207
                     /product="uncharacterized protein LOC144114300 isoform X1"
                     /calculated_mol_wt=23047
     CDS             1..207
                     /gene="LOC144114300"
                     /coded_by="XM_077647934.1:2608..3231"
                     /db_xref="GeneID:144114300"
ORIGIN      
        1 mpwakttvfv iiissslcpl qdkglsyasp vnpglcqlwq pypckllyli llvivlsakm
       61 hhtmrnfmys anidwhsmcs gtskcqgmdg wqtsatyiml davllllflg qltsrrycld
      121 gpkmnvwesg vaafftepvd ayslptdwpr ypspltleat sqfveqhgam spiqllekgv
      181 ipadtvghdt aamhpysrml lvqlhft
//
LOCUS       XP_077504061             207 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114300 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504061
VERSION     XP_077504061.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647935.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..207
                     /product="uncharacterized protein LOC144114300 isoform X1"
                     /calculated_mol_wt=23047
     CDS             1..207
                     /gene="LOC144114300"
                     /coded_by="XM_077647935.1:2608..3231"
                     /db_xref="GeneID:144114300"
ORIGIN      
        1 mpwakttvfv iiissslcpl qdkglsyasp vnpglcqlwq pypckllyli llvivlsakm
       61 hhtmrnfmys anidwhsmcs gtskcqgmdg wqtsatyiml davllllflg qltsrrycld
      121 gpkmnvwesg vaafftepvd ayslptdwpr ypspltleat sqfveqhgam spiqllekgv
      181 ipadtvghdt aamhpysrml lvqlhft
//
LOCUS       XP_077504062             207 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114300 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504062
VERSION     XP_077504062.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647936.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..207
                     /product="uncharacterized protein LOC144114300 isoform X1"
                     /calculated_mol_wt=23047
     CDS             1..207
                     /gene="LOC144114300"
                     /coded_by="XM_077647936.1:2543..3166"
                     /db_xref="GeneID:144114300"
ORIGIN      
        1 mpwakttvfv iiissslcpl qdkglsyasp vnpglcqlwq pypckllyli llvivlsakm
       61 hhtmrnfmys anidwhsmcs gtskcqgmdg wqtsatyiml davllllflg qltsrrycld
      121 gpkmnvwesg vaafftepvd ayslptdwpr ypspltleat sqfveqhgam spiqllekgv
      181 ipadtvghdt aamhpysrml lvqlhft
//
LOCUS       XP_077504063             120 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114300 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504063
VERSION     XP_077504063.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647937.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..120
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..120
                     /product="uncharacterized protein LOC144114300 isoform X2"
                     /calculated_mol_wt=13286
     CDS             1..120
                     /gene="LOC144114300"
                     /coded_by="XM_077647937.1:1747..2109"
                     /db_xref="GeneID:144114300"
ORIGIN      
        1 mdgwqtsaty imldavllll flgqltsrry cldgpkmnvw esgvaaffte pvdayslptd
       61 wprypspltl eatsqfveqh gamspiqlle kgvipadtvg hdtaamhpys rmllvqlhft
//
LOCUS       XP_077504064             120 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114300 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504064
VERSION     XP_077504064.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647938.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..120
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..120
                     /product="uncharacterized protein LOC144114300 isoform X2"
                     /calculated_mol_wt=13286
     CDS             1..120
                     /gene="LOC144114300"
                     /coded_by="XM_077647938.1:1874..2236"
                     /db_xref="GeneID:144114300"
ORIGIN      
        1 mdgwqtsaty imldavllll flgqltsrry cldgpkmnvw esgvaaffte pvdayslptd
       61 wprypspltl eatsqfveqh gamspiqlle kgvipadtvg hdtaamhpys rmllvqlhft
//
LOCUS       XP_077504065             120 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114300 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504065
VERSION     XP_077504065.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647939.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..120
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..120
                     /product="uncharacterized protein LOC144114300 isoform X2"
                     /calculated_mol_wt=13286
     CDS             1..120
                     /gene="LOC144114300"
                     /coded_by="XM_077647939.1:1807..2169"
                     /db_xref="GeneID:144114300"
ORIGIN      
        1 mdgwqtsaty imldavllll flgqltsrry cldgpkmnvw esgvaaffte pvdayslptd
       61 wprypspltl eatsqfveqh gamspiqlle kgvipadtvg hdtaamhpys rmllvqlhft
//
LOCUS       XP_077504066             535 aa            linear   INV 25-NOV-2025
DEFINITION  TOX high mobility group box family member 3-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077504066
VERSION     XP_077504066.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647940.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..535
                     /product="TOX high mobility group box family member 3-like
                     isoform X1"
                     /calculated_mol_wt=56510
     Region          263..332
                     /region_name="HMG-box_TOX-like"
                     /note="high mobility group (HMG)-box found in the TOX high
                     mobility group box family; cd21995"
                     /db_xref="CDD:438811"
     Site            order(263..264,266..270,273..274,281,293..294,297,
                     300..301,308,312,315,319,322)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438811"
     CDS             1..535
                     /gene="LOC144114302"
                     /coded_by="XM_077647940.1:475..2082"
                     /db_xref="GeneID:144114302"
ORIGIN      
        1 mntdgqsvfg tsgkdattae vsaqamdnfd lslsmqdqgq esagtqsyld qtfhtpsfgd
       61 eefeippinl qnvggpthpd eaataevvyg sqpgapmapq tsmaqalgsp maggmmehpq
      121 qhsypgpqgh pdmyhhamgm ppppqsmhlq svpayegtym gqyqsppaap ppqgalmgpp
      181 smmrgpapra gsagsgsppg snhtspgqet tsedsddstp lsqlvggvkr pspeplmdng
      241 ggkaakkakm pkkkkkrdpn epqkpvsaya lffrdtqaai kgqnpnasfg evskivasmw
      301 dgleadhknv ykkkteaakk eylkalaayr aslvskaaad hsdtlyasgp vssssavpsp
      361 pqgslsplqk ksplltslme ggsphqymam sppqgmlnaq qqvwpnmhhm aqspsmmpqq
      421 psqqammqqh sppqqmmmap vsvaapsyta dlmsapppsl sspassgggm mdqqgpmpgg
      481 achqrcirng cpnpaieshd wdreycssec vvshcrdvft awvaqrqvgt nsfvk
//
LOCUS       XP_077504068             535 aa            linear   INV 25-NOV-2025
DEFINITION  TOX high mobility group box family member 3-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077504068
VERSION     XP_077504068.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647942.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..535
                     /product="TOX high mobility group box family member 3-like
                     isoform X1"
                     /calculated_mol_wt=56510
     Region          263..332
                     /region_name="HMG-box_TOX-like"
                     /note="high mobility group (HMG)-box found in the TOX high
                     mobility group box family; cd21995"
                     /db_xref="CDD:438811"
     Site            order(263..264,266..270,273..274,281,293..294,297,
                     300..301,308,312,315,319,322)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438811"
     CDS             1..535
                     /gene="LOC144114302"
                     /coded_by="XM_077647942.1:416..2023"
                     /db_xref="GeneID:144114302"
ORIGIN      
        1 mntdgqsvfg tsgkdattae vsaqamdnfd lslsmqdqgq esagtqsyld qtfhtpsfgd
       61 eefeippinl qnvggpthpd eaataevvyg sqpgapmapq tsmaqalgsp maggmmehpq
      121 qhsypgpqgh pdmyhhamgm ppppqsmhlq svpayegtym gqyqsppaap ppqgalmgpp
      181 smmrgpapra gsagsgsppg snhtspgqet tsedsddstp lsqlvggvkr pspeplmdng
      241 ggkaakkakm pkkkkkrdpn epqkpvsaya lffrdtqaai kgqnpnasfg evskivasmw
      301 dgleadhknv ykkkteaakk eylkalaayr aslvskaaad hsdtlyasgp vssssavpsp
      361 pqgslsplqk ksplltslme ggsphqymam sppqgmlnaq qqvwpnmhhm aqspsmmpqq
      421 psqqammqqh sppqqmmmap vsvaapsyta dlmsapppsl sspassgggm mdqqgpmpgg
      481 achqrcirng cpnpaieshd wdreycssec vvshcrdvft awvaqrqvgt nsfvk
//
LOCUS       XP_077504069             530 aa            linear   INV 25-NOV-2025
DEFINITION  TOX high mobility group box family member 3-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077504069
VERSION     XP_077504069.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647943.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..530
                     /product="TOX high mobility group box family member 3-like
                     isoform X2"
                     /calculated_mol_wt=55882
     Region          263..332
                     /region_name="HMG-box_TOX-like"
                     /note="high mobility group (HMG)-box found in the TOX high
                     mobility group box family; cd21995"
                     /db_xref="CDD:438811"
     Site            order(263..264,266..270,273..274,281,293..294,297,
                     300..301,308,312,315,319,322)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438811"
     CDS             1..530
                     /gene="LOC144114302"
                     /coded_by="XM_077647943.1:477..2069"
                     /db_xref="GeneID:144114302"
ORIGIN      
        1 mntdgqsvfg tsgkdattae vsaqamdnfd lslsmqdqgq esagtqsyld qtfhtpsfgd
       61 eefeippinl qnvggpthpd eaataevvyg sqpgapmapq tsmaqalgsp maggmmehpq
      121 qhsypgpqgh pdmyhhamgm ppppqsmhlq svpayegtym gqyqsppaap ppqgalmgpp
      181 smmrgpapra gsagsgsppg snhtspgqet tsedsddstp lsqlvggvkr pspeplmdng
      241 ggkaakkakm pkkkkkrdpn epqkpvsaya lffrdtqaai kgqnpnasfg evskivasmw
      301 dgleadhknv ykkkteaakk eylkalaayr aslvskaaad hsdtlyasgp vssssavpsp
      361 pqgslsplqk ksplltslme ggsphqymam sppqgmlnaq qqhhmaqsps mmpqqpsqqa
      421 mmqqhsppqq mmmapvsvaa psytadlmsa pppslsspas sgggmmdqqg pmpggachqr
      481 cirngcpnpa ieshdwdrey cssecvvshc rdvftawvaq rqvgtnsfvk
//
LOCUS       XP_077504070             527 aa            linear   INV 25-NOV-2025
DEFINITION  TOX high mobility group box family member 3-like isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077504070
VERSION     XP_077504070.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647944.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..527
                     /product="TOX high mobility group box family member 3-like
                     isoform X3"
                     /calculated_mol_wt=55760
     Region          255..324
                     /region_name="HMG-box_TOX-like"
                     /note="high mobility group (HMG)-box found in the TOX high
                     mobility group box family; cd21995"
                     /db_xref="CDD:438811"
     Site            order(255..256,258..262,265..266,273,285..286,289,
                     292..293,300,304,307,311,314)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438811"
     CDS             1..527
                     /gene="LOC144114302"
                     /coded_by="XM_077647944.1:477..2060"
                     /db_xref="GeneID:144114302"
ORIGIN      
        1 mntdgqsvfg tsgkdattae vsaqamdnfd lslsmqdqgq esagtqsyld qtfhtpsfgd
       61 eefeippinl qnvggpthpd eaataevvyg sqtsmaqalg spmaggmmeh pqqhsypgpq
      121 ghpdmyhham gmppppqsmh lqsvpayegt ymgqyqsppa apppqgalmg ppsmmrgpap
      181 ragsagsgsp pgsnhtspgq ettsedsdds tplsqlvggv krpspeplmd ngggkaakka
      241 kmpkkkkkrd pnepqkpvsa yalffrdtqa aikgqnpnas fgevskivas mwdgleadhk
      301 nvykkkteaa kkeylkalaa yraslvskaa adhsdtlyas gpvssssavp sppqgslspl
      361 qkksplltsl meggsphqym amsppqgmln aqqqvwpnmh hmaqspsmmp qqpsqqammq
      421 qhsppqqmmm apvsvaapsy tadlmsappp slsspassgg gmmdqqgpmp ggachqrcir
      481 ngcpnpaies hdwdreycss ecvvshcrdv ftawvaqrqv gtnsfvk
//
LOCUS       XP_077504071             523 aa            linear   INV 25-NOV-2025
DEFINITION  TOX high mobility group box family member 3-like isoform X4
            [Amblyomma americanum].
ACCESSION   XP_077504071
VERSION     XP_077504071.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647945.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..523
                     /product="TOX high mobility group box family member 3-like
                     isoform X4"
                     /calculated_mol_wt=55411
     Region          251..320
                     /region_name="HMG-box_TOX-like"
                     /note="high mobility group (HMG)-box found in the TOX high
                     mobility group box family; cd21995"
                     /db_xref="CDD:438811"
     Site            order(251..252,254..258,261..262,269,281..282,285,
                     288..289,296,300,303,307,310)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438811"
     CDS             1..523
                     /gene="LOC144114302"
                     /coded_by="XM_077647945.1:384..1955"
                     /db_xref="GeneID:144114302"
ORIGIN      
        1 mlvvgeagqt lwamdnfdls lsmqdqgqes agtqsyldqt fhtpsfgdee feippinlqn
       61 vggpthpdea ataevvygsq pgapmapqts maqalgspma ggmmehpqqh sypgpqghpd
      121 myhhamgmpp ppqsmhlqsv payegtymgq yqsppaappp qgalmgppsm mrgpaprags
      181 agsgsppgsn htspgqetts edsddstpls qlvggvkrps peplmdnggg kaakkakmpk
      241 kkkkrdpnep qkpvsayalf frdtqaaikg qnpnasfgev skivasmwdg leadhknvyk
      301 kkteaakkey lkalaayras lvskaaadhs dtlyasgpvs sssavpsppq gslsplqkks
      361 plltslmegg sphqymamsp pqgmlnaqqq vwpnmhhmaq spsmmpqqps qqammqqhsp
      421 pqqmmmapvs vaapsytadl msapppslss passgggmmd qqgpmpggac hqrcirngcp
      481 npaieshdwd reycssecvv shcrdvftaw vaqrqvgtns fvk
//
LOCUS       XP_077504072             515 aa            linear   INV 25-NOV-2025
DEFINITION  TOX high mobility group box family member 3-like isoform X5
            [Amblyomma americanum].
ACCESSION   XP_077504072
VERSION     XP_077504072.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647946.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..515
                     /product="TOX high mobility group box family member 3-like
                     isoform X5"
                     /calculated_mol_wt=54661
     Region          243..312
                     /region_name="HMG-box_TOX-like"
                     /note="high mobility group (HMG)-box found in the TOX high
                     mobility group box family; cd21995"
                     /db_xref="CDD:438811"
     Site            order(243..244,246..250,253..254,261,273..274,277,
                     280..281,288,292,295,299,302)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438811"
     CDS             1..515
                     /gene="LOC144114302"
                     /coded_by="XM_077647946.1:384..1931"
                     /db_xref="GeneID:144114302"
ORIGIN      
        1 mlvvgeagqt lwamdnfdls lsmqdqgqes agtqsyldqt fhtpsfgdee feippinlqn
       61 vggpthpdea ataevvygsq tsmaqalgsp maggmmehpq qhsypgpqgh pdmyhhamgm
      121 ppppqsmhlq svpayegtym gqyqsppaap ppqgalmgpp smmrgpapra gsagsgsppg
      181 snhtspgqet tsedsddstp lsqlvggvkr pspeplmdng ggkaakkakm pkkkkkrdpn
      241 epqkpvsaya lffrdtqaai kgqnpnasfg evskivasmw dgleadhknv ykkkteaakk
      301 eylkalaayr aslvskaaad hsdtlyasgp vssssavpsp pqgslsplqk ksplltslme
      361 ggsphqymam sppqgmlnaq qqvwpnmhhm aqspsmmpqq psqqammqqh sppqqmmmap
      421 vsvaapsyta dlmsapppsl sspassgggm mdqqgpmpgg achqrcirng cpnpaieshd
      481 wdreycssec vvshcrdvft awvaqrqvgt nsfvk
//
LOCUS       XP_077504073             510 aa            linear   INV 25-NOV-2025
DEFINITION  TOX high mobility group box family member 3-like isoform X6
            [Amblyomma americanum].
ACCESSION   XP_077504073
VERSION     XP_077504073.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647947.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..510
                     /product="TOX high mobility group box family member 3-like
                     isoform X6"
                     /calculated_mol_wt=54054
     Region          238..307
                     /region_name="HMG-box_TOX-like"
                     /note="high mobility group (HMG)-box found in the TOX high
                     mobility group box family; cd21995"
                     /db_xref="CDD:438811"
     Site            order(238..239,241..245,248..249,256,268..269,272,
                     275..276,283,287,290,294,297)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438811"
     CDS             1..510
                     /gene="LOC144114302"
                     /coded_by="XM_077647947.1:349..1881"
                     /db_xref="GeneID:144114302"
ORIGIN      
        1 mdnfdlslsm qdqgqesagt qsyldqtfht psfgdeefei ppinlqnvgg pthpdeaata
       61 evvygsqpga pmapqtsmaq algspmaggm mehpqqhsyp gpqghpdmyh hamgmppppq
      121 smhlqsvpay egtymgqyqs ppaapppqga lmgppsmmrg papragsags gsppgsnhts
      181 pgqettseds ddstplsqlv ggvkrpspep lmdngggkaa kkakmpkkkk krdpnepqkp
      241 vsayalffrd tqaaikgqnp nasfgevski vasmwdglea dhknvykkkt eaakkeylka
      301 laayraslvs kaaadhsdtl yasgpvssss avpsppqgsl splqkkspll tslmeggsph
      361 qymamsppqg mlnaqqqvwp nmhhmaqsps mmpqqpsqqa mmqqhsppqq mmmapvsvaa
      421 psytadlmsa pppslsspas sgggmmdqqg pmpggachqr cirngcpnpa ieshdwdrey
      481 cssecvvshc rdvftawvaq rqvgtnsfvk
//
LOCUS       XP_077504074             509 aa            linear   INV 25-NOV-2025
DEFINITION  TOX high mobility group box family member 3-like isoform X7
            [Amblyomma americanum].
ACCESSION   XP_077504074
VERSION     XP_077504074.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647948.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..509
                     /product="TOX high mobility group box family member 3-like
                     isoform X7"
                     /calculated_mol_wt=54028
     Region          263..332
                     /region_name="HMG-box_TOX-like"
                     /note="high mobility group (HMG)-box found in the TOX high
                     mobility group box family; cd21995"
                     /db_xref="CDD:438811"
     Site            order(263..264,266..270,273..274,281,293..294,297,
                     300..301,308,312,315,319,322)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438811"
     CDS             1..509
                     /gene="LOC144114302"
                     /coded_by="XM_077647948.1:477..2006"
                     /db_xref="GeneID:144114302"
ORIGIN      
        1 mntdgqsvfg tsgkdattae vsaqamdnfd lslsmqdqgq esagtqsyld qtfhtpsfgd
       61 eefeippinl qnvggpthpd eaataevvyg sqpgapmapq tsmaqalgsp maggmmehpq
      121 qhsypgpqgh pdmyhhamgm ppppqsmhlq svpayegtym gqyqsppaap ppqgalmgpp
      181 smmrgpapra gsagsgsppg snhtspgqet tsedsddstp lsqlvggvkr pspeplmdng
      241 ggkaakkakm pkkkkkrdpn epqkpvsaya lffrdtqaai kgqnpnasfg evskivasmw
      301 dgleadhknv ykkkteaakk eylkalaayr aslvskgsls plqkkspllt slmeggsphq
      361 ymamsppqgm lnaqqqvwpn mhhmaqspsm mpqqpsqqam mqqhsppqqm mmapvsvaap
      421 sytadlmsap ppslsspass gggmmdqqgp mpggachqrc irngcpnpai eshdwdreyc
      481 ssecvvshcr dvftawvaqr qvgtnsfvk
//
LOCUS       XP_077504075             504 aa            linear   INV 25-NOV-2025
DEFINITION  TOX high mobility group box family member 3-like isoform X8
            [Amblyomma americanum].
ACCESSION   XP_077504075
VERSION     XP_077504075.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647949.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..504
                     /product="TOX high mobility group box family member 3-like
                     isoform X8"
                     /calculated_mol_wt=53400
     Region          263..332
                     /region_name="HMG-box_TOX-like"
                     /note="high mobility group (HMG)-box found in the TOX high
                     mobility group box family; cd21995"
                     /db_xref="CDD:438811"
     Site            order(263..264,266..270,273..274,281,293..294,297,
                     300..301,308,312,315,319,322)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438811"
     CDS             1..504
                     /gene="LOC144114302"
                     /coded_by="XM_077647949.1:479..1993"
                     /db_xref="GeneID:144114302"
ORIGIN      
        1 mntdgqsvfg tsgkdattae vsaqamdnfd lslsmqdqgq esagtqsyld qtfhtpsfgd
       61 eefeippinl qnvggpthpd eaataevvyg sqpgapmapq tsmaqalgsp maggmmehpq
      121 qhsypgpqgh pdmyhhamgm ppppqsmhlq svpayegtym gqyqsppaap ppqgalmgpp
      181 smmrgpapra gsagsgsppg snhtspgqet tsedsddstp lsqlvggvkr pspeplmdng
      241 ggkaakkakm pkkkkkrdpn epqkpvsaya lffrdtqaai kgqnpnasfg evskivasmw
      301 dgleadhknv ykkkteaakk eylkalaayr aslvskgsls plqkkspllt slmeggsphq
      361 ymamsppqgm lnaqqqhhma qspsmmpqqp sqqammqqhs ppqqmmmapv svaapsytad
      421 lmsapppsls spassgggmm dqqgpmpgga chqrcirngc pnpaieshdw dreycssecv
      481 vshcrdvfta wvaqrqvgtn sfvk
//
LOCUS       XP_077504076             497 aa            linear   INV 25-NOV-2025
DEFINITION  TOX high mobility group box family member 3-like isoform X9
            [Amblyomma americanum].
ACCESSION   XP_077504076
VERSION     XP_077504076.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647950.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..497
                     /product="TOX high mobility group box family member 3-like
                     isoform X9"
                     /calculated_mol_wt=52929
     Region          251..320
                     /region_name="HMG-box_TOX-like"
                     /note="high mobility group (HMG)-box found in the TOX high
                     mobility group box family; cd21995"
                     /db_xref="CDD:438811"
     Site            order(251..252,254..258,261..262,269,281..282,285,
                     288..289,296,300,303,307,310)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438811"
     CDS             1..497
                     /gene="LOC144114302"
                     /coded_by="XM_077647950.1:384..1877"
                     /db_xref="GeneID:144114302"
ORIGIN      
        1 mlvvgeagqt lwamdnfdls lsmqdqgqes agtqsyldqt fhtpsfgdee feippinlqn
       61 vggpthpdea ataevvygsq pgapmapqts maqalgspma ggmmehpqqh sypgpqghpd
      121 myhhamgmpp ppqsmhlqsv payegtymgq yqsppaappp qgalmgppsm mrgpaprags
      181 agsgsppgsn htspgqetts edsddstpls qlvggvkrps peplmdnggg kaakkakmpk
      241 kkkkrdpnep qkpvsayalf frdtqaaikg qnpnasfgev skivasmwdg leadhknvyk
      301 kkteaakkey lkalaayras lvskgslspl qkksplltsl meggsphqym amsppqgmln
      361 aqqqvwpnmh hmaqspsmmp qqpsqqammq qhsppqqmmm apvsvaapsy tadlmsappp
      421 slsspassgg gmmdqqgpmp ggachqrcir ngcpnpaies hdwdreycss ecvvshcrdv
      481 ftawvaqrqv gtnsfvk
//
LOCUS       XP_077504077             489 aa            linear   INV 25-NOV-2025
DEFINITION  TOX high mobility group box family member 3-like isoform X10
            [Amblyomma americanum].
ACCESSION   XP_077504077
VERSION     XP_077504077.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647951.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..489
                     /product="TOX high mobility group box family member 3-like
                     isoform X10"
                     /calculated_mol_wt=52179
     Region          243..312
                     /region_name="HMG-box_TOX-like"
                     /note="high mobility group (HMG)-box found in the TOX high
                     mobility group box family; cd21995"
                     /db_xref="CDD:438811"
     Site            order(243..244,246..250,253..254,261,273..274,277,
                     280..281,288,292,295,299,302)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438811"
     CDS             1..489
                     /gene="LOC144114302"
                     /coded_by="XM_077647951.1:384..1853"
                     /db_xref="GeneID:144114302"
ORIGIN      
        1 mlvvgeagqt lwamdnfdls lsmqdqgqes agtqsyldqt fhtpsfgdee feippinlqn
       61 vggpthpdea ataevvygsq tsmaqalgsp maggmmehpq qhsypgpqgh pdmyhhamgm
      121 ppppqsmhlq svpayegtym gqyqsppaap ppqgalmgpp smmrgpapra gsagsgsppg
      181 snhtspgqet tsedsddstp lsqlvggvkr pspeplmdng ggkaakkakm pkkkkkrdpn
      241 epqkpvsaya lffrdtqaai kgqnpnasfg evskivasmw dgleadhknv ykkkteaakk
      301 eylkalaayr aslvskgsls plqkkspllt slmeggsphq ymamsppqgm lnaqqqvwpn
      361 mhhmaqspsm mpqqpsqqam mqqhsppqqm mmapvsvaap sytadlmsap ppslsspass
      421 gggmmdqqgp mpggachqrc irngcpnpai eshdwdreyc ssecvvshcr dvftawvaqr
      481 qvgtnsfvk
//
LOCUS       XP_077504078             484 aa            linear   INV 25-NOV-2025
DEFINITION  TOX high mobility group box family member 3-like isoform X11
            [Amblyomma americanum].
ACCESSION   XP_077504078
VERSION     XP_077504078.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647952.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..484
                     /product="TOX high mobility group box family member 3-like
                     isoform X11"
                     /calculated_mol_wt=51572
     Region          238..307
                     /region_name="HMG-box_TOX-like"
                     /note="high mobility group (HMG)-box found in the TOX high
                     mobility group box family; cd21995"
                     /db_xref="CDD:438811"
     Site            order(238..239,241..245,248..249,256,268..269,272,
                     275..276,283,287,290,294,297)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438811"
     CDS             1..484
                     /gene="LOC144114302"
                     /coded_by="XM_077647952.1:349..1803"
                     /db_xref="GeneID:144114302"
ORIGIN      
        1 mdnfdlslsm qdqgqesagt qsyldqtfht psfgdeefei ppinlqnvgg pthpdeaata
       61 evvygsqpga pmapqtsmaq algspmaggm mehpqqhsyp gpqghpdmyh hamgmppppq
      121 smhlqsvpay egtymgqyqs ppaapppqga lmgppsmmrg papragsags gsppgsnhts
      181 pgqettseds ddstplsqlv ggvkrpspep lmdngggkaa kkakmpkkkk krdpnepqkp
      241 vsayalffrd tqaaikgqnp nasfgevski vasmwdglea dhknvykkkt eaakkeylka
      301 laayraslvs kgslsplqkk splltslmeg gsphqymams ppqgmlnaqq qvwpnmhhma
      361 qspsmmpqqp sqqammqqhs ppqqmmmapv svaapsytad lmsapppsls spassgggmm
      421 dqqgpmpgga chqrcirngc pnpaieshdw dreycssecv vshcrdvfta wvaqrqvgtn
      481 sfvk
//
LOCUS       XP_077504080             496 aa            linear   INV 25-NOV-2025
DEFINITION  Y+L amino acid transporter 2-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504080
VERSION     XP_077504080.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647954.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..496
                     /product="Y+L amino acid transporter 2-like isoform X1"
                     /calculated_mol_wt=53702
     Region          22..493
                     /region_name="2A0308"
                     /note="L-type amino acid transporter; TIGR00911"
                     /db_xref="CDD:273332"
     CDS             1..496
                     /gene="LOC144114305"
                     /coded_by="XM_077647954.1:302..1792"
                     /db_xref="GeneID:144114305"
ORIGIN      
        1 mppepsaesq lmnpdvqvrr gsgsespkgn svtlkkelgl mngvgmivgv iigagifvsp
       61 tgvlrfagst gmalvvwaac glismigaic yaelgtmipk sggdyayift afgplpaflf
      121 lwvalliiqp tsnaiagitf anyilepiya gcsppdnavr lvaavvicll tfincynvkw
      181 atfvqdslmf akvialviii iagfvhlaqg ntgnlsnmfe gtttdpgric lsfyaglfsy
      241 agwnylnfvt eelknpfrnl pfaiyislpm vtiiyllani syfvvltade vqsanavavt
      301 fgekvlgvvs wimpvsvals tfgglnggif assrlffvga rqghlpscla minvthftpa
      361 pslvflclls llyltntdvf vlitytafse amfimlsvgg llwlrikqpn tkrpikvnii
      421 lpvvfflisl flvvlpffsq pletsigagi mlsgipvyfl tiywkdkpla yrktihvvte
      481 yvqrilysvp qedkva
//
LOCUS       XP_077504081             501 aa            linear   INV 25-NOV-2025
DEFINITION  Y+L amino acid transporter 2-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504081
VERSION     XP_077504081.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647955.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..501
                     /product="Y+L amino acid transporter 2-like isoform X2"
                     /calculated_mol_wt=54577
     Region          35..498
                     /region_name="2A0308"
                     /note="L-type amino acid transporter; TIGR00911"
                     /db_xref="CDD:273332"
     CDS             1..501
                     /gene="LOC144114305"
                     /coded_by="XM_077647955.1:16..1521"
                     /db_xref="GeneID:144114305"
ORIGIN      
        1 maengfqset spllpkgpsi qqdvlpvdrt wtstvelkkr lslsngvsmi vgtivgagif
       61 vspagvmrya gsiglalvvw vltglaclig alcytelgtm ipksggdyay isaafgpfpa
      121 flqlwtmlvi vqpagnaiva ltfanyvlrp fyddvdchpp ssavmliaal vlclltfinc
      181 ynvkwatfvq dslmfakvia lviiiiagfv hlaqgntgnl snmfegtttd pgriclsfya
      241 glfsyagwny lnfvteelkn pfrnlpfaiy islpmvtiiy llanisyfvv ltadevqsan
      301 avavtfgekv lgvvswimpv svalstfggl nggifassrl ffvgarqghl psclaminvt
      361 hftpapslvf lcllsllylt ntdvfvlity tafseamfim lsvggllwlr ikqpntkrpi
      421 kvniilpvvf flislflvvl pffsqplets igagimlsgi pvyfltiywk dkplayrkti
      481 hvvteyvqri lysvpqedkv a
//
LOCUS       XP_077504082             307 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114306 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504082
VERSION     XP_077504082.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647956.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..307
                     /product="uncharacterized protein LOC144114306 isoform X1"
                     /calculated_mol_wt=34068
     Region          15..94
                     /region_name="Myb_DNA-bind_5"
                     /note="Myb/SANT-like DNA-binding domain; pfam13873"
                     /db_xref="CDD:433544"
     CDS             1..307
                     /gene="LOC144114306"
                     /coded_by="XM_077647956.1:401..1324"
                     /db_xref="GeneID:144114306"
ORIGIN      
        1 myyhiekcme slepktpswt endllvlite ywkrkdilra kasesvtnlq krecwieite
       61 vvnarcftph tkktmdqlkr kwektimlak kaalniqkrs gnlsdlapay qlalsivced
      121 fpaslecang laaadvpsla ehpnaggyid evssmvitel eeessgdgpp lpkgdkaqpl
      181 kadvlgeaae gaphplgsrg ypalsagsed gaadghhpqq heahdasaae fvnnfrkrkw
      241 eqdlielqke kvrrqiqyhk vqhelqmsvl rsqmefwemk ksklaaeirq ggiptsppse
      301 danasat
//
LOCUS       XP_077504083             299 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114306 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504083
VERSION     XP_077504083.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647957.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..299
                     /product="uncharacterized protein LOC144114306 isoform X2"
                     /calculated_mol_wt=32999
     Region          7..86
                     /region_name="Myb_DNA-bind_5"
                     /note="Myb/SANT-like DNA-binding domain; pfam13873"
                     /db_xref="CDD:433544"
     CDS             1..299
                     /gene="LOC144114306"
                     /coded_by="XM_077647957.1:555..1454"
                     /db_xref="GeneID:144114306"
ORIGIN      
        1 meslepktps wtendllvli teywkrkdil rakasesvtn lqkrecwiei tevvnarcft
       61 phtkktmdql krkwektiml akkaalniqk rsgnlsdlap ayqlalsivc edfpasleca
      121 nglaaadvps laehpnaggy idevssmvit eleeessgdg pplpkgdkaq plkadvlgea
      181 aegaphplgs rgypalsags edgaadghhp qqheahdasa aefvnnfrkr kweqdlielq
      241 kekvrrqiqy hkvqhelqms vlrsqmefwe mkksklaaei rqggiptspp sedanasat
//
LOCUS       XP_077504084             299 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114306 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504084
VERSION     XP_077504084.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647958.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..299
                     /product="uncharacterized protein LOC144114306 isoform X2"
                     /calculated_mol_wt=32999
     Region          7..86
                     /region_name="Myb_DNA-bind_5"
                     /note="Myb/SANT-like DNA-binding domain; pfam13873"
                     /db_xref="CDD:433544"
     CDS             1..299
                     /gene="LOC144114306"
                     /coded_by="XM_077647958.1:608..1507"
                     /db_xref="GeneID:144114306"
ORIGIN      
        1 meslepktps wtendllvli teywkrkdil rakasesvtn lqkrecwiei tevvnarcft
       61 phtkktmdql krkwektiml akkaalniqk rsgnlsdlap ayqlalsivc edfpasleca
      121 nglaaadvps laehpnaggy idevssmvit eleeessgdg pplpkgdkaq plkadvlgea
      181 aegaphplgs rgypalsags edgaadghhp qqheahdasa aefvnnfrkr kweqdlielq
      241 kekvrrqiqy hkvqhelqms vlrsqmefwe mkksklaaei rqggiptspp sedanasat
//
LOCUS       XP_077504085             477 aa            linear   INV 25-NOV-2025
DEFINITION  large neutral amino acids transporter small subunit 2-like
            [Amblyomma americanum].
ACCESSION   XP_077504085
VERSION     XP_077504085.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647959.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..477
                     /product="large neutral amino acids transporter small
                     subunit 2-like"
                     /calculated_mol_wt=52356
     Region          2..468
                     /region_name="2A0308"
                     /note="L-type amino acid transporter; TIGR00911"
                     /db_xref="CDD:273332"
     CDS             1..477
                     /gene="LOC144114308"
                     /coded_by="XM_077647959.1:125..1558"
                     /db_xref="GeneID:144114308"
ORIGIN      
        1 maddegrvkl kkeiglfsgv mivvgtiigs gifvsptgvf khagsvgasl viwvlcglfs
       61 mmgavcyael gtsiprsggd yayvleafgp ltaflrlwvt vlvvqpatla vlsltfatym
      121 vkplypdcep pdlalrlmai vclcfltfvn cksvklamkv qdifttakla alaliivtgm
      181 vriaqgqvey lqnsfageys vagislsfya glfafggwny lnyvseelkd pnknlpraiy
      241 igitlvtvvy vlanvayfta vspqemlasp avavtfaqrt igvvawimpv fvslstfggl
      301 ngimftiarl ffigaleghl pmifgmihtt kltptpplll scavallmfa tsdifvliny
      361 lsfnqwlwvg vsilgmlwlr ykrpnmhrpi kvplffpvvf lamctfltfm plyasptetg
      421 mglvilitgi payyifviws vknktvrkfs eyltiemqky levilpeqee klleaet
//
LOCUS       XP_077504086             138 aa            linear   INV 25-NOV-2025
DEFINITION  adult-specific rigid cuticular protein 15.7-like [Amblyomma
            americanum].
ACCESSION   XP_077504086
VERSION     XP_077504086.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647960.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..138
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..138
                     /product="adult-specific rigid cuticular protein
                     15.7-like"
                     /calculated_mol_wt=14633
     Region          41..92
                     /region_name="Chitin_bind_4"
                     /note="Insect cuticle protein; pfam00379"
                     /db_xref="CDD:459790"
     CDS             1..138
                     /gene="LOC144114309"
                     /coded_by="XM_077647960.1:99..515"
                     /db_xref="GeneID:144114309"
ORIGIN      
        1 mvaqlrttvl falvavalgg yhgghgghsk qyrkqddygh yrfgydivng ygaingrhet
       61 gsaygpvhgs yylgdidgrh rqvhyvadkw gframvktne pgtktslpaa apyhsangkh
      121 vpaygigggh ggghgyhg
//
LOCUS       XP_077504087             169 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114311 [Amblyomma americanum].
ACCESSION   XP_077504087
VERSION     XP_077504087.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647961.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..169
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..169
                     /product="uncharacterized protein LOC144114311"
                     /calculated_mol_wt=17251
     Region          42..93
                     /region_name="Chitin_bind_4"
                     /note="Insect cuticle protein; pfam00379"
                     /db_xref="CDD:459790"
     CDS             1..169
                     /gene="LOC144114311"
                     /coded_by="XM_077647961.1:41..550"
                     /db_xref="GeneID:144114311"
ORIGIN      
        1 miaqltvvla laavvfaggy gghghhggts ktyrkqndhg hysfgydivn gygavngrhe
       61 tgsaygpvhg syylgdidgr hrqvhyvadk lgfravvktn epgtksslpa aapyhsahgk
      121 svpayghggy ggyggyggyg gyggyggygh ggyggyggyg gygghgyyg
//
LOCUS       XP_077504088             417 aa            linear   INV 25-NOV-2025
DEFINITION  protein sidekick-like [Amblyomma americanum].
ACCESSION   XP_077504088
VERSION     XP_077504088.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647962.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..417
                     /product="protein sidekick-like"
                     /calculated_mol_wt=45324
     Region          26..95
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          116..191
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          132..137
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          146..150
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          168..172
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          182..187
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          216..292
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          315..403
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          333..337
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          346..350
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          368..373
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          383..388
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          396..399
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..417
                     /gene="LOC144114312"
                     /coded_by="XM_077647962.1:43..1296"
                     /db_xref="GeneID:144114312"
ORIGIN      
        1 maqmsdvily swqgrgkrrc ehqlsprflv epaggyaglg erralrceav gapaprlqll
       61 kdgepltnwt aqrlvhvlss ishsqagsyq claenaagal lsakarlrva hltraeevpe
      121 tvpvnarkgg dvilapplvd svppatavwt rldgrnldsr nfaqtqdnrl vildvspkda
      181 gqyrveltnp htgdnlsgpv veltvddnee dqaelsivvp psdrefnnlg ngydstleci
      241 asgrpldqvq iewlkdgral gelphvlthw nrtltllrlg pghtgryscq vtlrhapeds
      301 savvahanvt vsvlpslsqk vneetavelg qqvrlpcvae ghpepqvhwl ldarpvdqst
      361 ghfhvgdggs leigalamed agvyqcvaen plgearastw lhvkrtchlp stfhypw
//
LOCUS       XP_077504089            1181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114313 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504089
VERSION     XP_077504089.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647963.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1181
                     /product="uncharacterized protein LOC144114313 isoform X1"
                     /calculated_mol_wt=131431
     Region          779..1139
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(874..877,880,882,893,895,942,988..991,1032,
                     1036..1037,1039)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..1181
                     /gene="LOC144114313"
                     /coded_by="XM_077647963.1:51..3596"
                     /db_xref="GeneID:144114313"
ORIGIN      
        1 micceqttqq sgialsqadk nagkrrqgvk rpptklslks krqqtrqppp tsqkklnvqq
       61 cnsselgnrs sgissceilr hhpqktdsyt sdktppatcp pllvqcggsr kisiiegsaq
      121 prgrtlrdnl ewssmgdtdf lnlsslsgms stlmstrnfp senceevsrv ashlgcgtis
      181 pivlrenngr sheqhctads lassflisgn qtnwvttrkr nrkkswhege enkkiarlcf
      241 krqeisnsar dtsalknwap llastpvsrs vkvtkarkew flgcavrtph naapfdisgs
      301 vlapdsftsq rkaqkgaadr tknglrrkls galrfqvipr dncnnlsqkn aetpalatrn
      361 qgcersplss clstevacta vppthcgsta essaplqakd kmkdlrvmls dilvevphaq
      421 ntplnafsqk gdmsdlqgka elcitetnnn qrakrkqclk qspclasegt aqqpsfqkel
      481 rvklvdimkv anmlecpqye pspfaqrdvk htpmllkdre lkkkfhlkea rvvltdlrvq
      541 gtkinlqdsm rlcsnplkge vqhvtctkve gcdmqynsst tfaklsfncs tvtdmcvkai
      601 pqsynkqfel hfaehsggkr pkitstntsi pehsatelsr misstaccte akfsqsqvel
      661 gataaslnms rkqetqyssm dnynlgslsa aaqtqeivst veqtlhfity gagtsfkhhs
      721 yfenllesgv aahlacdrts dsisdraaee fgllkelkvv lekcdhhqlm lpppsvvnkn
      781 ksqhkdsqrs ncnpkqsits pqhdlgqdee pckngnwtdc vrrqarltma rksvvpspvl
      841 ldagqallem cnqnetltfe qalgrevlrl ckkigegtfg evfriklksk asaikivpie
      901 gsfpvngeeq ktaaeilpea iicqelsalc qqkshascpn fievkrlyyi qdcyhralle
      961 qwdvynakre senerpdfsk adqkflmfef adggisledy evkcatvaks iflqvacala
     1021 vaetalefeh rdlhwgnilv aptkrpsirc hlpqgdfemd tggvlvslid ytlsrmckgg
     1081 aiiftdvskd kalfsgsgdy qfdiyrhmkq hnqndwksfr pytnvlwlhy lsrklldkty
     1141 cvrsrqqsst laelrqwsda vvlkcpsake vflkcvnkqp l
//
LOCUS       XP_077504091            1245 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114313 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504091
VERSION     XP_077504091.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647965.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1245
                     /product="uncharacterized protein LOC144114313 isoform X2"
                     /calculated_mol_wt=138632
     Region          843..1203
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(938..941,944,946,957,959,1006,1052..1055,1096,
                     1100..1101,1103)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..1245
                     /gene="LOC144114313"
                     /coded_by="XM_077647965.1:316..4053"
                     /db_xref="GeneID:144114313"
ORIGIN      
        1 mqecrcavqc cstqqeaqtk yrhesnvfds steqsgsdhg eksgsapghl anqvhckrrk
       61 gnhnprrrkq ttqqsgials qadknagkrr qgvkrpptkl slkskrqqtr qppptsqkkl
      121 nvqqcnssel gnrssgissc eilrhhpqkt dsytsdktpp atcppllvqc ggsrkisiie
      181 gsaqprgrtl rdnlewssmg dtdflnlssl sgmsstlmst rnfpsencee vsrvashlgc
      241 gtispivlre nngrsheqhc tadslassfl isgnqtnwvt trkrnrkksw hegeenkkia
      301 rlcfkrqeis nsardtsalk nwapllastp vsrsvkvtka rkewflgcav rtphnaapfd
      361 isgsvlapds ftsqrkaqkg aadrtknglr rklsgalrfq viprdncnnl sqknaetpal
      421 atrnqgcers plssclstev actavppthc gstaessapl qakdkmkdlr vmlsdilvev
      481 phaqntplna fsqkgdmsdl qgkaelcite tnnnqrakrk qclkqspcla segtaqqpsf
      541 qkelrvklvd imkvanmlec pqyepspfaq rdvkhtpmll kdrelkkkfh lkearvvltd
      601 lrvqgtkinl qdsmrlcsnp lkgevqhvtc tkvegcdmqy nssttfakls fncstvtdmc
      661 vkaipqsynk qfelhfaehs ggkrpkitst ntsipehsat elsrmissta ccteakfsqs
      721 qvelgataas lnmsrkqetq yssmdnynlg slsaaaqtqe ivstveqtlh fitygagtsf
      781 khhsyfenll esgvaahlac drtsdsisdr aaeefgllke lkvvlekcdh hqlmlpppsv
      841 vnknksqhkd sqrsncnpkq sitspqhdlg qdeepckngn wtdcvrrqar ltmarksvvp
      901 spvlldagqa llemcnqnet ltfeqalgre vlrlckkige gtfgevfrik lkskasaiki
      961 vpiegsfpvn geeqktaaei lpeaiicqel salcqqksha scpnfievkr lyyiqdcyhr
     1021 alleqwdvyn akresenerp dfskadqkfl mfefadggis ledyevkcat vaksiflqva
     1081 calavaetal efehrdlhwg nilvaptkrp sirchlpqgd femdtggvlv slidytlsrm
     1141 ckggaiiftd vskdkalfsg sgdyqfdiyr hmkqhnqndw ksfrpytnvl wlhylsrkll
     1201 dktycvrsrq qsstlaelrq wsdavvlkcp sakevflkcv nkqpl
//
LOCUS       XP_077504092            1181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114313 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504092
VERSION     XP_077504092.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647966.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1181
                     /product="uncharacterized protein LOC144114313 isoform X1"
                     /calculated_mol_wt=131431
     Region          779..1139
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(874..877,880,882,893,895,942,988..991,1032,
                     1036..1037,1039)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..1181
                     /gene="LOC144114313"
                     /coded_by="XM_077647966.1:220..3765"
                     /db_xref="GeneID:144114313"
ORIGIN      
        1 micceqttqq sgialsqadk nagkrrqgvk rpptklslks krqqtrqppp tsqkklnvqq
       61 cnsselgnrs sgissceilr hhpqktdsyt sdktppatcp pllvqcggsr kisiiegsaq
      121 prgrtlrdnl ewssmgdtdf lnlsslsgms stlmstrnfp senceevsrv ashlgcgtis
      181 pivlrenngr sheqhctads lassflisgn qtnwvttrkr nrkkswhege enkkiarlcf
      241 krqeisnsar dtsalknwap llastpvsrs vkvtkarkew flgcavrtph naapfdisgs
      301 vlapdsftsq rkaqkgaadr tknglrrkls galrfqvipr dncnnlsqkn aetpalatrn
      361 qgcersplss clstevacta vppthcgsta essaplqakd kmkdlrvmls dilvevphaq
      421 ntplnafsqk gdmsdlqgka elcitetnnn qrakrkqclk qspclasegt aqqpsfqkel
      481 rvklvdimkv anmlecpqye pspfaqrdvk htpmllkdre lkkkfhlkea rvvltdlrvq
      541 gtkinlqdsm rlcsnplkge vqhvtctkve gcdmqynsst tfaklsfncs tvtdmcvkai
      601 pqsynkqfel hfaehsggkr pkitstntsi pehsatelsr misstaccte akfsqsqvel
      661 gataaslnms rkqetqyssm dnynlgslsa aaqtqeivst veqtlhfity gagtsfkhhs
      721 yfenllesgv aahlacdrts dsisdraaee fgllkelkvv lekcdhhqlm lpppsvvnkn
      781 ksqhkdsqrs ncnpkqsits pqhdlgqdee pckngnwtdc vrrqarltma rksvvpspvl
      841 ldagqallem cnqnetltfe qalgrevlrl ckkigegtfg evfriklksk asaikivpie
      901 gsfpvngeeq ktaaeilpea iicqelsalc qqkshascpn fievkrlyyi qdcyhralle
      961 qwdvynakre senerpdfsk adqkflmfef adggisledy evkcatvaks iflqvacala
     1021 vaetalefeh rdlhwgnilv aptkrpsirc hlpqgdfemd tggvlvslid ytlsrmckgg
     1081 aiiftdvskd kalfsgsgdy qfdiyrhmkq hnqndwksfr pytnvlwlhy lsrklldkty
     1141 cvrsrqqsst laelrqwsda vvlkcpsake vflkcvnkqp l
//
LOCUS       XP_077504093             281 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114314 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504093
VERSION     XP_077504093.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647967.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..281
                     /product="uncharacterized protein LOC144114314 isoform X1"
                     /calculated_mol_wt=31901
     CDS             1..281
                     /gene="LOC144114314"
                     /coded_by="XM_077647967.1:117..962"
                     /db_xref="GeneID:144114314"
ORIGIN      
        1 mdrscdaaga sakeqmsrev niwlcemlge gheiymdekg vtamhklmtl qkaaqkdald
       61 qmvileertk afnakaekle atlsaahiap qqlpesvrsh lkdlsyvatl lgcqdghpqr
      121 ieekllslhe qrkelrskel elkediakfk eliqttknql asarasmrhi spndeecrmd
      181 awektdqkyq arlaafekrr aqgplpqgpg rllslseklk rlalkeriln eklqpmadlp
      241 pdekaietml eaaertrefl tkalekqlkk erakheaaqk t
//
LOCUS       XP_077504094             258 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114314 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504094
VERSION     XP_077504094.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647968.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..258
                     /product="uncharacterized protein LOC144114314 isoform X2"
                     /calculated_mol_wt=29232
     CDS             1..258
                     /gene="LOC144114314"
                     /coded_by="XM_077647968.1:117..893"
                     /db_xref="GeneID:144114314"
ORIGIN      
        1 mdrscdaaga sakeqmsrev niwlcemlge gheiymdekg vtamhklmtl qkaaqkdald
       61 qmvileertk afnakaekle atlsaahiap qqlpesvrsh lkdlsyvatl lgcqdghpqr
      121 ieekllslhe qrkelrskel elkediakfk eliqttknql asarasmrhi spndeecrmd
      181 awektdqkyq arlaafeqkr raqgplpqgp grllslsede kaietmleaa ertrefltka
      241 lekqlkkera kheaaqkt
//
LOCUS       XP_077504095             257 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114314 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504095
VERSION     XP_077504095.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647969.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..257
                     /product="uncharacterized protein LOC144114314 isoform X3"
                     /calculated_mol_wt=29103
     Region          47..>256
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     CDS             1..257
                     /gene="LOC144114314"
                     /coded_by="XM_077647969.1:117..890"
                     /db_xref="GeneID:144114314"
ORIGIN      
        1 mdrscdaaga sakeqmsrev niwlcemlge gheiymdekg vtamhklmtl qkaaqkdald
       61 qmvileertk afnakaekle atlsaahiap qqlpesvrsh lkdlsyvatl lgcqdghpqr
      121 ieekllslhe qrkelrskel elkediakfk eliqttknql asarasmrhi spndeecrmd
      181 awektdqkyq arlaafekrr aqgplpqgpg rllslsedek aietmleaae rtrefltkal
      241 ekqlkkerak heaaqkt
//
LOCUS       XP_077504096             256 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114314 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077504096
VERSION     XP_077504096.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647970.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..256
                     /product="uncharacterized protein LOC144114314 isoform X4"
                     /calculated_mol_wt=29146
     CDS             1..256
                     /gene="LOC144114314"
                     /coded_by="XM_077647970.1:104..874"
                     /db_xref="GeneID:144114314"
ORIGIN      
        1 mlgegheiym dekgvtamhk lmtlqkaaqk daldqmvile ertkafnaka ekleatlsaa
       61 hiapqqlpes vrshlkdlsy vatllgcqdg hpqrieekll slheqrkelr skelelkedi
      121 akfkeliqtt knqlasaras mrhispndee crmdawektd qkyqarlaaf eqkrraqgpl
      181 pqgpgrllsl seklkrlalk erilneklqp madlppdeka ietmleaaer trefltkale
      241 kqlkkerakh eaaqkt
//
LOCUS       XP_077504097             282 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114314 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077504097
VERSION     XP_077504097.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647971.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..282
                     /product="uncharacterized protein LOC144114314 isoform X5"
                     /calculated_mol_wt=32029
     CDS             1..282
                     /gene="LOC144114314"
                     /coded_by="XM_077647971.1:131..979"
                     /db_xref="GeneID:144114314"
ORIGIN      
        1 mdrscdaaga sakeqmsrev niwlcemlge gheiymdekg vtamhklmtl qkaaqkdald
       61 qmvileertk afnakaekle atlsaahiap qqlpesvrsh lkdlsyvatl lgcqdghpqr
      121 ieekllslhe qrkelrskel elkediakfk eliqttknql asarasmrhi spndeecrmd
      181 awektdqkyq arlaafeqkr raqgplpqgp grllslsekl krlalkeril neklqpmadl
      241 ppdekaietm leaaertref ltkalekqlk kerakheaaq kt
//
LOCUS       XP_077504098             455 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114318 [Amblyomma americanum].
ACCESSION   XP_077504098
VERSION     XP_077504098.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647972.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..455
                     /product="uncharacterized protein LOC144114318"
                     /calculated_mol_wt=49566
     CDS             1..455
                     /gene="LOC144114318"
                     /coded_by="XM_077647972.1:3149..4516"
                     /db_xref="GeneID:144114318"
ORIGIN      
        1 matgssssga tplcgtypna yspnplhdds emdsrdtspq nqdllssssv enassplggg
       61 pgwqhalnkr rakklrrqar qaqnpstpds sasvggnpps tsgqssnair ppgthrpklq
      121 plpkndikvv irptrglalk typtrvisaa lvhscrdptk vkgkyilrph vgskilvist
      181 lheetaaelc kithltldks phpvrayvpf pattlrgvih gldpqetrdt lvpgvrirdn
      241 rielldaril gssttalltl egtqlprsvy ydgceypcft yrparqfchi cmqpghcpdv
      301 cptpkvrvcr lcgaqdppit hqcqpkcglc acqhetgsre clkrlkpart prpatnkgak
      361 kmthqsrkda gpriynnrwf ssddeettgc nqsssrsrsr snsrprtktp vpppalpaad
      421 egkrnkktpp qgqkqvswae kvqgppapsn dprfe
//
LOCUS       XP_077504099             388 aa            linear   INV 25-NOV-2025
DEFINITION  large neutral amino acids transporter small subunit 1-like
            [Amblyomma americanum].
ACCESSION   XP_077504099
VERSION     XP_077504099.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647973.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..388
                     /product="large neutral amino acids transporter small
                     subunit 1-like"
                     /calculated_mol_wt=42871
     Region          <62..385
                     /region_name="2A0308"
                     /note="L-type amino acid transporter; TIGR00911"
                     /db_xref="CDD:273332"
     CDS             1..388
                     /gene="LOC144114319"
                     /coded_by="XM_077647973.1:278..1444"
                     /db_xref="GeneID:144114319"
ORIGIN      
        1 mnslqrearl qlapassspl hhphrqfqav hcgggssrtr rwtsclgrqg aqqtslrhat
       61 glltfincyn vkwatfvqds lmfakvialv iiviagfvhl aqgntgnlsn mfegtttdpg
      121 liclsfyvgl fpyagwnyln fvteelknpf rnlpfaiyis lpmvtiiyll anisyfvvlt
      181 adevqsanav avtfeekvlg vvswimpvsv alstfgglng gifassrlff vgarqghlps
      241 claminmthf tpapslvflc llsllyltnt nvfalityta fseamfimls vggllwlrik
      301 qpntkrpikv niilpvvfli slflvvlpfs sqpletsiga gimlsgipvy fltiywkdkp
      361 layrktiyvv teyvqrilys vpqedkvg
//
LOCUS       XP_077504101             500 aa            linear   INV 25-NOV-2025
DEFINITION  Y+L amino acid transporter 2-like [Amblyomma americanum].
ACCESSION   XP_077504101
VERSION     XP_077504101.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647975.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..500
                     /product="Y+L amino acid transporter 2-like"
                     /calculated_mol_wt=54409
     Region          36..497
                     /region_name="2A0308"
                     /note="L-type amino acid transporter; TIGR00911"
                     /db_xref="CDD:273332"
     CDS             1..500
                     /gene="LOC144114322"
                     /coded_by="XM_077647975.1:244..1746"
                     /db_xref="GeneID:144114322"
ORIGIN      
        1 maengfqset spllpkgpsi qqdvlpidrt wtstmelkkr lglsngvsmi vgtivgagif
       61 vspagvilys gsiglalvvw vltglaclig alcytelgtm ipksggdyay isaafgpfpa
      121 flqlwtmlvi vkpagnaiva ltfanyvlrp fyddvdchpp ssavmliaal vlclltfinc
      181 ynvkwatfvq dslmfakvia lviiviagfv hlaqgntgnl snmfegtttd pgliclsfyv
      241 glfpyagwny lnfvteelkn pfrnlpfaiy islpmvtiiy llanisyfvv ltadevqsan
      301 avavtfeekv lgvvswimpv svalstfggl nggifassrl ffvgarqghl psclaminmt
      361 hftpapslvf lcllsllylt ntnvfality tafseamfim lsvggllwlr ikqpntkrpi
      421 kvniilpvvf lislflvvlp fssqpletsi gagimlsgip vyfltiywkd kplayrktiy
      481 vvteyvqril ysvpqedkvg
//
LOCUS       XP_077504102             307 aa            linear   INV 25-NOV-2025
DEFINITION  large neutral amino acids transporter small subunit 1-like
            [Amblyomma americanum].
ACCESSION   XP_077504102
VERSION     XP_077504102.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647976.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..307
                     /product="large neutral amino acids transporter small
                     subunit 1-like"
                     /calculated_mol_wt=33852
     Region          <2..304
                     /region_name="2A0308"
                     /note="L-type amino acid transporter; TIGR00911"
                     /db_xref="CDD:273332"
     CDS             1..307
                     /gene="LOC144114324"
                     /coded_by="XM_077647976.1:510..1433"
                     /db_xref="GeneID:144114324"
ORIGIN      
        1 mfakvialvi iviagfvhla qgntgnlsnm fegtttdpgl iclsfyvglf pyagwnylnf
       61 vteelknpfr nlpfaiyisl pmvtiiylla nisyfvvlta devqsanava vtfeekvlgv
      121 vswimpvsva lstfgglngg ifassrlffv garqghlpsc laminrthft papslvflcl
      181 lsllyltntn vfalitytaf seamfimlsv ggllwlrikq pntkrpikvn iilpvvflis
      241 lflvvlpfss qpletsigag imlsgipvyf ltiywkdkpl ayrktiyvvt eyvqrilysv
      301 pqedkvg
//
LOCUS       XP_077504103             548 aa            linear   INV 25-NOV-2025
DEFINITION  chromatin licensing and DNA replication factor double parked
            [Amblyomma americanum].
ACCESSION   XP_077504103
VERSION     XP_077504103.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647977.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..548
                     /product="chromatin licensing and DNA replication factor
                     double parked"
                     /calculated_mol_wt=60871
     Site            order(189..191,193..194,197..200,203,219,249,302..304,307,
                     310,329,338,342..349)
                     /site_type="active"
                     /note="Cdt1-Geminin inhibitory interaction site [active]"
                     /db_xref="CDD:176571"
     Site            order(190..191,193..194,197..200,202,338,342..349,351)
                     /site_type="active"
                     /note="Cdt1-Geminin permissive interaction site [active]"
                     /db_xref="CDD:176571"
     Region          207..364
                     /region_name="CDT1"
                     /note="DNA replication factor CDT1 like; pfam08839"
                     /db_xref="CDD:462618"
     Region          418..542
                     /region_name="Cdt1_c"
                     /note="The C-terminal fold of replication licensing factor
                     Cdt1 is essential for Cdt1 activity and directly interacts
                     with MCM2-7 helicase; cd08767"
                     /db_xref="CDD:176572"
     Site            order(435,439,475..476,480,502,506,510,517,519,522,540)
                     /site_type="active"
                     /note="putative Cdt1-MCM complex binding site [active]"
                     /db_xref="CDD:176572"
     CDS             1..548
                     /gene="dup"
                     /coded_by="XM_077647977.1:103..1749"
                     /db_xref="GeneID:144114326"
ORIGIN      
        1 mslmkqaalt ryysakkqgs alkdketgpf gnsidalrtp qtvtqalrsa asgtaslphk
       61 rrrigaaekt pvtskqgdsv pawrppssvr krlllheete kgdvpsfaee tiatlhtsvl
      121 eeteekhvts plkqtpdavq qkavlmkkla asgklqelqa rlraiddakt alssaqtvkk
      181 aqyktvedpa yvrfhhltep rppsltlpys yselaeifrc cdtivrllfn rkevctfdkl
      241 rrsveemskk sfskaklgqi tavfpdayil sqekvlqrgv gdggyqlvvt pklppeggkl
      301 ltaahllhrr khfhsvllsa vkehhekfll sltppvniss diltrwhpkf pldsvpaisp
      361 aalpesplkr sfssaqdvld kvkgrlgeri ekallglsag qqeavvtvmp srndevastc
      421 lkgisedlla riraresfkl vkemtlhped ekeqaelakl pefiritrsh flaeqkaail
      481 kddlvqkire sfssiqavae veslitlask vfpdwfkvlt irrgtfvkiv retdinglia
      541 rvtnklkk
//
LOCUS       XP_077504104             166 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114327 [Amblyomma americanum].
ACCESSION   XP_077504104
VERSION     XP_077504104.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647978.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..166
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..166
                     /product="uncharacterized protein LOC144114327"
                     /calculated_mol_wt=17863
     Region          <1..85
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     CDS             1..166
                     /gene="LOC144114327"
                     /coded_by="XM_077647978.1:112..612"
                     /db_xref="GeneID:144114327"
ORIGIN      
        1 mgirlgldll ltlcpspprs allcdsraal srlqsngrgt plvrdirssi erlaqrgcav
       61 raqwvpghcg iagneeaddl ataahqlppt dlslaledvr avihdhlrkq hpdphiaggg
      121 sgyvtggeer dtkdfyanyp ldtfgnliik rskawrlafs svetct
//
LOCUS       XP_077504105             482 aa            linear   INV 25-NOV-2025
DEFINITION  neuropilin and tolloid-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077504105
VERSION     XP_077504105.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647979.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..482
                     /product="neuropilin and tolloid-like protein 1"
                     /calculated_mol_wt=53920
     Region          59..163
                     /region_name="CUB"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; cd00041"
                     /db_xref="CDD:238001"
     Site            order(59,61,90,95,134,158,160,162..163)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     Region          <208..294
                     /region_name="CUB"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; cd00041"
                     /db_xref="CDD:238001"
     Region          304..337
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(310,318,329..330)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(322,325,329,335..336)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            332..336
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     CDS             1..482
                     /gene="LOC144114330"
                     /coded_by="XM_077647979.1:375..1823"
                     /db_xref="GeneID:144114330"
ORIGIN      
        1 mrrspgrlav aalavtllti tssmiagkei ksfkwgelde verarichnf tegdkvrqel
       61 ysplyngtnh ypnntectrl irapynymvr ldfrdrfhle esptcefdyl eirngayays
      121 plearlcgdt fpkpvqsegr hlwlkfssda sieytgfkav ytfkpapkth epppeeclfn
      181 vegshgiigs edikpsqleh srqnklplec twsirvkpsw kmyltfleyk ladpnncrsn
      241 fvdilaeepq heslshfcgt maeptqsksn hvkvryyaeh galkggnfsi lftafrevss
      301 gescdpdtef scddgwcidv klkcngqfnc kyrydeessi chvgsnvgvv lsmkhmvvil
      361 vvfsalvvgm casigitchn kiqerrrrer eyklrrskea svevgldlsa gnleaalalq
      421 aaeigarrag glppgtpaat tlvgptvlrp edddngcyvp evdlsvfrkh pngrplspat
      481 ea
//
LOCUS       XP_077504106             162 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114331 [Amblyomma americanum].
ACCESSION   XP_077504106
VERSION     XP_077504106.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647980.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..162
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..162
                     /product="uncharacterized protein LOC144114331"
                     /calculated_mol_wt=17601
     Region          36..148
                     /region_name="DUF3421"
                     /note="Protein of unknown function (DUF3421); pfam11901"
                     /db_xref="CDD:463390"
     CDS             1..162
                     /gene="LOC144114331"
                     /coded_by="XM_077647980.1:138..626"
                     /db_xref="GeneID:144114331"
ORIGIN      
        1 madadysvtk yqcccewvkc fenrlpyhal sagtlngqdv yvaravhege tligwaqpan
       61 sccyvswsgg ahahaeyqcl atetpdkmgw vpasdgelps gavhggraad neplfvgrvh
      121 tndgvlvgkv qpshrtlyva hdgqelnfkd yevlvckrle lc
//
LOCUS       XP_077504108             874 aa            linear   INV 25-NOV-2025
DEFINITION  paramyosin [Amblyomma americanum].
ACCESSION   XP_077504108
VERSION     XP_077504108.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647982.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..874
                     /product="paramyosin"
                     /calculated_mol_wt=101877
     Region          <28..847
                     /region_name="Myosin_tail_1"
                     /note="Myosin tail; pfam01576"
                     /db_xref="CDD:460256"
     CDS             1..874
                     /gene="Prm"
                     /coded_by="XM_077647982.1:255..2879"
                     /db_xref="GeneID:144114332"
ORIGIN      
        1 msssrsskym ykssggagdi sieygtdlga ltrledklrl lqedlesere lrqriereks
       61 dltvqlmqls drleeaegss etvvemnkkr dtelsklrkl ledvhlesee tahhlrkkhq
      121 eaiaefqeql dvmtkakska ekerqkfqae vyellaqven ttkekitiqk tveklehtvy
      181 elnirieeln rtvtevtaqr trlsaenaey lkevhelkvs ldnvnhlktq latqledtrr
      241 rledeerkra slesslhtle veieslkvql eeesearlev erqlvkanad aaayktkyet
      301 evqahadeve elrrkmaqki seyeeqleal ltrcsnlekq ksrlqsevev limdlekata
      361 haqnlekrva qleklnidlk skveeltill eqsqrelrqk vaeiqklqhe yekmreqrda
      421 lqrenkklvd dlseaksqla dairrlheye leikrlener delaaaykea etlrkqeeak
      481 cqrltaelaq vrheyerrlq akdeeiealr kqyqleveql nmrlaeaeak lkteiarikk
      541 kyqaqitele msldaankqn ldlqkiikkq avqitelqah ydevhrqlqq cadqlavsqr
      601 rcqglqaeld eqrvalesal rskraaeqsl eesqarvnel ttinvniaaa knkleselsa
      661 lqadydelhk elrvvdercq rtivelkstk dilveeqery ikvesikksl evevrnlqvr
      721 leeveanala ggkrviakle arirdveiem eeekkrhaet qkmlrkkdhr akelllqtee
      781 dhktitmlnd aveklnekvk vykrqlneqe glsqqnltrv rrfqreleaa edradsaesn
      841 lslirakhrs wvttsqvpgg trqvfvteet sqnf
//
LOCUS       XP_077504109             333 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114333 [Amblyomma americanum].
ACCESSION   XP_077504109
VERSION     XP_077504109.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647983.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..333
                     /product="uncharacterized protein LOC144114333"
                     /calculated_mol_wt=36343
     CDS             1..333
                     /gene="LOC144114333"
                     /coded_by="XM_077647983.1:164..1165"
                     /db_xref="GeneID:144114333"
ORIGIN      
        1 mtpvaaypsl vsgrggpssa rpllsrapiv pkesdwfcsc rqpralaaea arlgvndtsr
       61 cpgtahvmah lassrriwet ailespfpaa srnvtgktrd splknvdlpg qdvetssyqq
      121 rlscrgplqh mqggsvwprv lktarlkaaf lpsvaarrhc rhdsplpekp lqdqqrthlr
      181 wwqhalrett lqscvecrqg eprrgrtepr pppaparpgr rratesssdi pgaarsycwr
      241 rgrerrsslr ggahsgrcsv saagtrlvcy sllctaeasg rhrrpvaapa atphrwgrgd
      301 cssapdsard iacrrghslp pnwllvsvdr aat
//
LOCUS       XP_077504110             196 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114334 [Amblyomma americanum].
ACCESSION   XP_077504110
VERSION     XP_077504110.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647984.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..196
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..196
                     /product="uncharacterized protein LOC144114334"
                     /calculated_mol_wt=23999
     CDS             1..196
                     /gene="LOC144114334"
                     /coded_by="XM_077647984.1:58..648"
                     /db_xref="GeneID:144114334"
ORIGIN      
        1 mgtrrrqpyl hmvydrnqqy gdslyrktvd dlylregimp prallasrrf ddsyfmtryp
       61 ripsmyrllk rwdtgddlal rrsmslshit hpdynralln rllypdehls rrfgnqhklh
      121 vhsghdrhvs amedhrslma elelqevqdr lrrehnrkwf npdsivyevp syrddplatv
      181 yrrrrpfsyy dfyywl
//
LOCUS       XP_077504111            1043 aa            linear   INV 25-NOV-2025
DEFINITION  membrane-bound transcription factor site-1 protease isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077504111
VERSION     XP_077504111.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647985.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1043
                     /product="membrane-bound transcription factor site-1
                     protease isoform X1"
                     /calculated_mol_wt=116518
     Region          180..433
                     /region_name="Peptidases_S8_SKI-1_like"
                     /note="Peptidase S8 family domain in SKI-1-like proteins;
                     cd07479"
                     /db_xref="CDD:173805"
     Site            order(195,226,266,285,315,391)
                     /site_type="active"
                     /db_xref="CDD:173805"
     Site            order(195,226,391)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:173805"
     CDS             1..1043
                     /gene="S1P"
                     /coded_by="XM_077647985.1:38..3169"
                     /db_xref="GeneID:144114335"
ORIGIN      
        1 msglkaqlad smcnvshaek rvllqystsv vrneyivtfk hyyrrnardk fidaalrasg
       61 ignwerlpre naaadfpsdf elikllegtv eeglaalkdh plvkyvtpqr svtrtlkfln
      121 aseahckdfs cwhssrpfst rnslawesaf aheperspqr klmrviprqi tkalqadvlw
      181 nlgirgakvk vavfdtglpk nhphfkkvke rtnwtnekml ddglghgtfv agviassrdc
      241 lgfapeselh vyrvfnkdql sytswfldaf nyailkkinv lnlsiggpdf mdhpfvdkvw
      301 eltannvimi saigndgply gtlnnpadqm dvigvgginf edqiarfssr gmttwelpgg
      361 ygrlkpdivt ygssvrgsav sgscrslsgt svaspvvaga vtllmsgalh lgsalnpasv
      421 kqalmasagr lqgfpmfeqg wgklqlleay rtlvnyrpqa tlspsyldls ecpymwpyct
      481 qplyhgalpi ianitilngm gvsgrivgkp ewhpyvpehg hylelafgyp evlwpwsgfl
      541 sihfvvtaea aqwegiaqgh vsltvesspg egeteprrsn lklpikakii ptpprskril
      601 wdqyhnlryp pgyfprdnlk iktdpldwsg dhihtnfkdm yqhlrnhgyy ievlgfpftc
      661 fdaqnygtll lvdleeeyfp eelaklhrdv eaglslvvfa dwynvsvmrk ikffdentrq
      721 wwmpdtggsn vpaindlltp wniglgdnvy egeftigdhk myyasgtsii kfpengllir
      781 rtltnqgqev lekkadrveg vpilglvqtg sskssgriai ygdsncidtt hiqkdcywll
      841 halvqysttg litpffettn aatlpsdpll pkrmegnrly ryskvfqnhp gapelralpp
      901 cqtlnwavpq plnesapsil wksqkllsve plplgagagg mvdvadelaf palkqrwggw
      961 alqpdeddsr qavsaseyfs pvrlaleqrr tvlgfgalta avfgwllyrr cchraatrra
     1021 qgrkgrarrr ilslpiskll rgv
//
LOCUS       XP_077504112            1032 aa            linear   INV 25-NOV-2025
DEFINITION  membrane-bound transcription factor site-1 protease isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077504112
VERSION     XP_077504112.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647986.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1032
                     /product="membrane-bound transcription factor site-1
                     protease isoform X2"
                     /calculated_mol_wt=115441
     Region          169..422
                     /region_name="Peptidases_S8_SKI-1_like"
                     /note="Peptidase S8 family domain in SKI-1-like proteins;
                     cd07479"
                     /db_xref="CDD:173805"
     Site            order(184,215,255,274,304,380)
                     /site_type="active"
                     /db_xref="CDD:173805"
     Site            order(184,215,380)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:173805"
     CDS             1..1032
                     /gene="S1P"
                     /coded_by="XM_077647986.1:130..3228"
                     /db_xref="GeneID:144114335"
ORIGIN      
        1 mcnvihaeqr vllqystsvv rneyivtfkh yyrrnardkf idaalrasgi gnwerlpren
       61 aaadfpsdfe likllegtve eglaalkdhp lvkyvtpqrs vtrtlkflna seahckdfsc
      121 whssrpfstr nslawesafa heperspqrk lmrviprqit kalqadvlwn lgirgakvkv
      181 avfdtglpkn hphfkkvker tnwtnekmld dglghgtfva gviassrdcl gfapeselhv
      241 yrvfnkdqls ytswfldafn yailkkinvl nlsiggpdfm dhpfvdkvwe ltannvimis
      301 aigndgplyg tlnnpadqmd vigvgginfe dqiarfssrg mttwelpggy grlkpdivty
      361 gssvrgsavs gscrslsgts vaspvvagav tllmsgalhl gsalnpasvk qalmasagrl
      421 qgfpmfeqgw gklqlleayr tlvnyrpqat lspsyldlse cpymwpyctq plyhgalpii
      481 anitilngmg vsgrivgkpe whpyvpehgh ylelafgype vlwpwsgfls ihfvvtaeaa
      541 qwegiaqghv sltvesspge geteprrsnl klpikakiip tpprskrilw dqyhnlrypp
      601 gyfprdnlki ktdpldwsgd hihtnfkdmy qhlrnhgyyi evlgfpftcf daqnygtlll
      661 vdleeeyfpe elaklhrdve aglslvvfad wynvsvmrki kffdentrqw wmpdtggsnv
      721 paindlltpw niglgdnvye geftigdhkm yyasgtsiik fpengllirr tltnqgqevl
      781 ekkadrvegv pilglvqtgs skssgriaiy gdsncidtth iqkdcywllh alvqysttgl
      841 itpffettna atlpsdpllp krmegnrlyr yskvfqnhpg apelralppc qtlnwavpqp
      901 lnesapsilw ksqkllsvep lplgagaggm vdvadelafp alkqrwggwa lqpdeddsrq
      961 avsaseyfsp vrlaleqrrt vlgfgaltaa vfgwllyrrc chraatrraq grkgrarrri
     1021 lslpiskllr gv
//
LOCUS       XP_077504113             320 aa            linear   INV 25-NOV-2025
DEFINITION  NADH dehydrogenase (ubiquinone) complex I, assembly factor 6
            homolog sicily [Amblyomma americanum].
ACCESSION   XP_077504113
VERSION     XP_077504113.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647987.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..320
                     /product="NADH dehydrogenase (ubiquinone) complex I,
                     assembly factor 6 homolog sicily"
                     /calculated_mol_wt=35977
     Region          54..304
                     /region_name="SQS_PSY"
                     /note="Squalene/phytoene synthase; pfam00494"
                     /db_xref="CDD:425717"
     Site            order(61,80,86..87,90,174,199..200,204,208,212)
                     /site_type="other"
                     /note="substrate binding pocket [chemical binding]"
                     /db_xref="CDD:173830"
     Site            order(90..91,204..205,208,212)
                     /site_type="active"
                     /note="substrate-Mg2+ binding site [active]"
                     /db_xref="CDD:173830"
     Site            90..93
                     /site_type="other"
                     /note="aspartate-rich region 1"
                     /db_xref="CDD:173830"
     Site            204..212
                     /site_type="other"
                     /note="aspartate-rich region 2"
                     /db_xref="CDD:173830"
     CDS             1..320
                     /gene="sicily"
                     /coded_by="XM_077647987.1:72..1034"
                     /db_xref="GeneID:144114336"
ORIGIN      
        1 mfwynlktaa rlgslqtecv cqrrlrtcls msrlfkssta krgtspaayc tdlvkqydye
       61 nylcslllpa gvrrsafair afnvelaqir dvvsradigl mrihfwkdav ervykgsppe
      121 hpvaqelsvl vhskqlsrqw mnrmidsres ilgdkphntv sdleeyaeks vsstlylilq
      181 smgvqsvacd hvashigklq gitnlirgip fnasrgrvyv pselmakhkv sqesvargkg
      241 dlkevvydia stahqhlvka rslekdtpss vravylpava sgayleklrq adfnvfdgrl
      301 hlrngllpls lwwhkvkrsf
//
LOCUS       XP_077504114            1035 aa            linear   INV 25-NOV-2025
DEFINITION  adenylate cyclase type 2 Ac76E isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504114
VERSION     XP_077504114.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647988.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1035
                     /product="adenylate cyclase type 2 Ac76E isoform X1"
                     /calculated_mol_wt=114824
     Region          50..422
                     /region_name="AcyC"
                     /note="Adenylate cyclase, class 3 [Signal transduction
                     mechanisms]; COG2114"
                     /db_xref="CDD:441717"
     Region          260..442
                     /region_name="Guanylate_cyc"
                     /note="Adenylate and Guanylate cyclase catalytic domain;
                     pfam00211"
                     /db_xref="CDD:425528"
     Site            order(272,274..279,314,316..318,321,383..385,389..390,
                     393..394)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(274,318)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(279,291,294..295,298,302,314..315,371,374,384..387,
                     390)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     Region          475..555
                     /region_name="Adcy_cons_dom"
                     /note="Adenylate cyclase, conserved domain; pfam06327"
                     /db_xref="CDD:461877"
     Region          830..1027
                     /region_name="Guanylate_cyc"
                     /note="Adenylate and Guanylate cyclase catalytic domain;
                     pfam00211"
                     /db_xref="CDD:425528"
     Site            order(842,844..849,891,893..895,898,969..971,975..976,
                     979..980,1016)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(844,895)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(849,865,868..869,872,876,891..892,957,960,970..973,
                     976,1016)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     CDS             1..1035
                     /gene="Ac76E"
                     /coded_by="XM_077647988.1:222..3329"
                     /db_xref="GeneID:144114337"
ORIGIN      
        1 mgpgsvdlsd lrrrysgrlr hslllallaa lvmlglaflv lvlllyphdf aqdlrrfvvl
       61 svallvalal lvllalvpae vllsrwalpl glvgwlllag ptlawtvake hispvdplap
      121 afvaafllhv llplrpiaal lmgpgssagl lvatalladw dtrhfgcqla agalflsmat
      181 avgwyhrhmr daaqsrsfas tqeciearik leyekeqqeq lllsvipayi aaevkrsiml
      241 kmadacqtsa krsrqrfhel yvqrhnnvsi lyadivnftr lseqlsasdl vqtlnelfgr
      301 fdqiaqenhc mrikilgdcy ycvsglpvsr pshayncvrm glqmieairv vreatevnvd
      361 mrigvhtgnv lcgvlglrkw qydvwsddvt lanhmesggv pgrvhitkat yhqldgkfet
      421 epgnghqrdq ylaehqvetf liapqkkets eespqavren anrhrstskm ykhvecwgad
      481 kpfanisett laknvgltsl alieanllpd sgvpwnfssl gncrrcspdg lnpvllrfed
      541 sslesqfqnq pdpqfpqcvv gsallftaiv ivqlltlqvh lmllcvlasa sivlgvltla
      601 actrqcqsmd retsthstai hpqcipglag nlllrilffi ttilfimsat finmitcgst
      661 ggehiiipsn esngsyatal ieeipydcgl vryqlpcaml altavsvylr infllkllam
      721 inglasfsla fiffrsdlyg dplepepgls nvphgayaal fltfyvvilh ildrqveyma
      781 rvdflsrakl rvehdemetm gsinkillen ilpahvashf lnnsrlsqel yherysnvav
      841 mfasipnyie fydetdvnkq gleclrllne iicdfdklll kpkfsvieki ktigstymaa
      901 aglqpgreen gdkprddhnv vslvefaial talleqinre sfqrfklrig inhgpviagv
      961 vgaqkpqydi wgntvnvasr mdscgvigri qvtedtakkl qecgyececr gstyvkgkgt
     1021 lttyfvktqf drnds
//
LOCUS       XP_077504115            1029 aa            linear   INV 25-NOV-2025
DEFINITION  adenylate cyclase type 2 Ac76E isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504115
VERSION     XP_077504115.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647989.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1029
                     /product="adenylate cyclase type 2 Ac76E isoform X2"
                     /calculated_mol_wt=114262
     Region          50..422
                     /region_name="AcyC"
                     /note="Adenylate cyclase, class 3 [Signal transduction
                     mechanisms]; COG2114"
                     /db_xref="CDD:441717"
     Region          260..442
                     /region_name="Guanylate_cyc"
                     /note="Adenylate and Guanylate cyclase catalytic domain;
                     pfam00211"
                     /db_xref="CDD:425528"
     Site            order(272,274..279,314,316..318,321,383..385,389..390,
                     393..394)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(274,318)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(279,291,294..295,298,302,314..315,371,374,384..387,
                     390)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     Region          475..549
                     /region_name="Adcy_cons_dom"
                     /note="Adenylate cyclase, conserved domain; pfam06327"
                     /db_xref="CDD:461877"
     Region          824..1021
                     /region_name="Guanylate_cyc"
                     /note="Adenylate and Guanylate cyclase catalytic domain;
                     pfam00211"
                     /db_xref="CDD:425528"
     Site            order(836,838..843,885,887..889,892,963..965,969..970,
                     973..974,1010)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(838,889)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(843,859,862..863,866,870,885..886,951,954,964..967,
                     970,1010)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     CDS             1..1029
                     /gene="Ac76E"
                     /coded_by="XM_077647989.1:281..3370"
                     /db_xref="GeneID:144114337"
ORIGIN      
        1 mgpgsvdlsd lrrrysgrlr hslllallaa lvmlglaflv lvlllyphdf aqdlrrfvvl
       61 svallvalal lvllalvpae vllsrwalpl glvgwlllag ptlawtvake hispvdplap
      121 afvaafllhv llplrpiaal lmgpgssagl lvatalladw dtrhfgcqla agalflsmat
      181 avgwyhrhmr daaqsrsfas tqeciearik leyekeqqeq lllsvipayi aaevkrsiml
      241 kmadacqtsa krsrqrfhel yvqrhnnvsi lyadivnftr lseqlsasdl vqtlnelfgr
      301 fdqiaqenhc mrikilgdcy ycvsglpvsr pshayncvrm glqmieairv vreatevnvd
      361 mrigvhtgnv lcgvlglrkw qydvwsddvt lanhmesggv pgrvhitkat yhqldgkfet
      421 epgnghqrdq ylaehqvetf liapqkkets eespqavren anrhrstskm ykhvecwgad
      481 kpfanisett laknvgltsl alieanllpd sgvpwnfrrc spdglnpvll rfedsslesq
      541 fqnqpdpqfp qcvvgsallf taivivqllt lqvhlmllcv lasasivlgv ltlaactrqc
      601 qsmdretsth staihpqcip glagnlllri lffittilfi msatfinmit cgstggehii
      661 ipsnesngsy atalieeipy dcglvryqlp camlaltavs vylrinfllk llaminglas
      721 fslafiffrs dlygdplepe pglsnvphga yaalfltfyv vilhildrqv eymarvdfls
      781 raklrvehde metmgsinki llenilpahv ashflnnsrl sqelyherys nvavmfasip
      841 nyiefydetd vnkqgleclr llneiicdfd klllkpkfsv iekiktigst ymaaaglqpg
      901 reengdkprd dhnvvslvef aialtalleq inresfqrfk lriginhgpv iagvvgaqkp
      961 qydiwgntvn vasrmdscgv igriqvtedt akklqecgye cecrgstyvk gkgtlttyfv
     1021 ktqfdrnds
//
LOCUS       XP_077504116             974 aa            linear   INV 25-NOV-2025
DEFINITION  adenylate cyclase type 2 Ac76E isoform X3 [Amblyomma americanum].
ACCESSION   XP_077504116
VERSION     XP_077504116.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647990.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..974
                     /product="adenylate cyclase type 2 Ac76E isoform X3"
                     /calculated_mol_wt=108207
     Region          50..422
                     /region_name="AcyC"
                     /note="Adenylate cyclase, class 3 [Signal transduction
                     mechanisms]; COG2114"
                     /db_xref="CDD:441717"
     Region          260..442
                     /region_name="Guanylate_cyc"
                     /note="Adenylate and Guanylate cyclase catalytic domain;
                     pfam00211"
                     /db_xref="CDD:425528"
     Site            order(272,274..279,314,316..318,321,383..385,389..390,
                     393..394)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(274,318)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(279,291,294..295,298,302,314..315,371,374,384..387,
                     390)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     Region          769..966
                     /region_name="Guanylate_cyc"
                     /note="Adenylate and Guanylate cyclase catalytic domain;
                     pfam00211"
                     /db_xref="CDD:425528"
     Site            order(781,783..788,830,832..834,837,908..910,914..915,
                     918..919,955)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(783,834)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(788,804,807..808,811,815,830..831,896,899,909..912,
                     915,955)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     CDS             1..974
                     /gene="Ac76E"
                     /coded_by="XM_077647990.1:222..3146"
                     /db_xref="GeneID:144114337"
ORIGIN      
        1 mgpgsvdlsd lrrrysgrlr hslllallaa lvmlglaflv lvlllyphdf aqdlrrfvvl
       61 svallvalal lvllalvpae vllsrwalpl glvgwlllag ptlawtvake hispvdplap
      121 afvaafllhv llplrpiaal lmgpgssagl lvatalladw dtrhfgcqla agalflsmat
      181 avgwyhrhmr daaqsrsfas tqeciearik leyekeqqeq lllsvipayi aaevkrsiml
      241 kmadacqtsa krsrqrfhel yvqrhnnvsi lyadivnftr lseqlsasdl vqtlnelfgr
      301 fdqiaqenhc mrikilgdcy ycvsglpvsr pshayncvrm glqmieairv vreatevnvd
      361 mrigvhtgnv lcgvlglrkw qydvwsddvt lanhmesggv pgrvhitkat yhqldgkfet
      421 epgnghqrdq ylaehqvetf liapqkkets eespqavren anrhrstskm ykhvecwgad
      481 kpfanisett laknvgltsl alieanllpd sgvpwnfrhl mllcvlasas ivlgvltlaa
      541 ctrqcqsmdr etsthstaih pqcipglagn lllrilffit tilfimsatf inmitcgstg
      601 gehiiipsne sngsyatali eeipydcglv ryqlpcamla ltavsvylri nfllkllami
      661 nglasfslaf iffrsdlygd plepepglsn vphgayaalf ltfyvvilhi ldrqveymar
      721 vdflsraklr vehdemetmg sinkilleni lpahvashfl nnsrlsqely herysnvavm
      781 fasipnyief ydetdvnkqg leclrllnei icdfdklllk pkfsviekik tigstymaaa
      841 glqpgreeng dkprddhnvv slvefaialt alleqinres fqrfklrigi nhgpviagvv
      901 gaqkpqydiw gntvnvasrm dscgvigriq vtedtakklq ecgyececrg styvkgkgtl
      961 ttyfvktqfd rnds
//
LOCUS       XP_077504117             913 aa            linear   INV 25-NOV-2025
DEFINITION  protein FAN-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504117
VERSION     XP_077504117.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647991.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..913
                     /product="protein FAN-like isoform X1"
                     /calculated_mol_wt=103044
     Region          182..279
                     /region_name="PH_BEACH"
                     /note="Pleckstrin homology domain in BEACH domain
                     containing proteins; cd01201"
                     /db_xref="CDD:275391"
     Region          295..568
                     /region_name="Beach"
                     /note="Beige/BEACH domain; pfam02138"
                     /db_xref="CDD:460459"
     Region          <651..909
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          717..756
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          761..797
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          804..839
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          844..876
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..913
                     /gene="LOC144114339"
                     /coded_by="XM_077647991.1:371..3112"
                     /db_xref="GeneID:144114339"
ORIGIN      
        1 mafieppnse rfsllllepg eiyfkdysvy lypdnlkeae aqrrrqkgrl kvcsksilfv
       61 pedinhpilk fplneasivk rwtgpllskl ysknsvmnlq ndqvvemleg nfikpyvfkr
      121 ekvsflfsli ygsideclmh isqlsrastl sppehdsmia amvlswqsri sfdrscledl
      181 yerivletka nkitplvinp glvlltslrl yfqpfnnvep wphikinlcd vkrvirrrfl
      241 lrqvglelyc sdssvlkhlf lsfktqqerd elykqltsqe alkledsgqe nmtllwqngv
      301 isnydyllyi nsladrsfnd ltqypvfpwv igdyksntld lsnsssyrdl srplgalsek
      361 rlkklkdrya emaepkflyg shystpgfvl yylvrkmpqy mlclqngrfd hpdrmfnsis
      421 dtwrnvttda adfkelipef ydpacngdfl vnslnldfgk rqngcavgdv elpqwaqgpa
      481 hfirtcreal essfvsenlh dwidlifgyk qtgeeavlad nvfyhltyeg svdldsiqdi
      541 yerhslevqi mefgqtpkqi fkhphpqrcc tvlprslsma sdscpdslle nvssipeele
      601 ardrapsiss fawkrnmadl tkmaehmlhk emvthvcfss skssvfsvsq dsllklysle
      661 qkqqlrsvni shmalssciv mddktvvvgs wdnyvyvynm eygritestl ghedavsdls
      721 wkngvlatas wdstakiwkl vahgdksgcs llaelehesg vacialdaeg ehlvsgtveg
      781 cvslwklqgs tcigqfdihk gtvsavsfnp dghriascse dkmlkvldvp tgtvlfskna
      841 gwamrclcwd gatvltgcds gsilvwdlls aklvatvsah sgpvtcihvc edgsliatgg
      901 sdgkvvvwqs aep
//
LOCUS       XP_077504118             908 aa            linear   INV 25-NOV-2025
DEFINITION  protein FAN-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504118
VERSION     XP_077504118.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647992.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..908
                     /product="protein FAN-like isoform X2"
                     /calculated_mol_wt=102375
     Region          177..274
                     /region_name="PH_BEACH"
                     /note="Pleckstrin homology domain in BEACH domain
                     containing proteins; cd01201"
                     /db_xref="CDD:275391"
     Region          290..563
                     /region_name="Beach"
                     /note="Beige/BEACH domain; pfam02138"
                     /db_xref="CDD:460459"
     Region          <646..904
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          712..751
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          756..792
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          799..834
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          839..871
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..908
                     /gene="LOC144114339"
                     /coded_by="XM_077647992.1:16..2742"
                     /db_xref="GeneID:144114339"
ORIGIN      
        1 matsgrfsll llepgeiyfk dysvylypdn lkeaeaqrrr qkgrlkvcsk silfvpedin
       61 hpilkfplne asivkrwtgp llsklyskns vmnlqndqvv emlegnfikp yvfkrekvsf
      121 lfsliygsid eclmhisqls rastlsppeh dsmiaamvls wqsrisfdrs cledlyeriv
      181 letkankitp lvinpglvll tslrlyfqpf nnvepwphik inlcdvkrvi rrrfllrqvg
      241 lelycsdssv lkhlflsfkt qqerdelykq ltsqealkle dsgqenmtll wqngvisnyd
      301 yllyinslad rsfndltqyp vfpwvigdyk sntldlsnss syrdlsrplg alsekrlkkl
      361 kdryaemaep kflygshyst pgfvlyylvr kmpqymlclq ngrfdhpdrm fnsisdtwrn
      421 vttdaadfke lipefydpac ngdflvnsln ldfgkrqngc avgdvelpqw aqgpahfirt
      481 crealessfv senlhdwidl ifgykqtgee avladnvfyh ltyegsvdld siqdiyerhs
      541 levqimefgq tpkqifkhph pqrcctvlpr slsmasdscp dsllenvssi peeleardra
      601 psissfawkr nmadltkmae hmlhkemvth vcfssskssv fsvsqdsllk lysleqkqql
      661 rsvnishmal sscivmddkt vvvgswdnyv yvynmeygri testlgheda vsdlswkngv
      721 lataswdsta kiwklvahgd ksgcsllael ehesgvacia ldaegehlvs gtvegcvslw
      781 klqgstcigq fdihkgtvsa vsfnpdghri ascsedkmlk vldvptgtvl fsknagwamr
      841 clcwdgatvl tgcdsgsilv wdllsaklva tvsahsgpvt cihvcedgsl iatggsdgkv
      901 vvwqsaep
//
LOCUS       XP_077504120             535 aa            linear   INV 25-NOV-2025
DEFINITION  protein FAN-like [Amblyomma americanum].
ACCESSION   XP_077504120
VERSION     XP_077504120.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647994.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..535
                     /product="protein FAN-like"
                     /calculated_mol_wt=58979
     Region          <21..190
                     /region_name="Beach"
                     /note="Beige/BEACH domain; pfam02138"
                     /db_xref="CDD:460459"
     Region          <273..531
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          297..333
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          339..378
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          383..419
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          426..461
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          466..498
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..535
                     /gene="LOC144114340"
                     /coded_by="XM_077647994.1:155..1762"
                     /db_xref="GeneID:144114340"
ORIGIN      
        1 mdgfwavkgv vlmnffkqvl qymlclqngr fdhsdrmfns isdtwrnvtt daadfkelip
       61 efydpacngd flvnslnldf gkrqngcavg nvelpqwaqg pahfirtcre alessfvsen
      121 lhdwidlifg ykqtgeeavl adnvfyhlty egsvnldsiq diyerhslev qimefgqtpk
      181 qifehprpqr cctvlprsls masdscpdsl lenvssipee letrdrapsi ssfawkrnma
      241 dltkmaehml hkkmvthvcf ssskssvfsv sqdsllklys leqkqqvrsv nishmalssc
      301 ivmndktvvv gswdnyvyvy nmeygrites tlghedavsd lswkngvlat aswdstakiw
      361 klvahgdksg csllaelehe sgvacialda egehlvsgtv egcvslwklq gstcigqfdi
      421 hkgtvsavsf spgwyqiasc sedkmlkvld vptgtvlfsk nagwamrclc wdgatvltgc
      481 dsgsilvwdl lsaklvatvs ahsgpvtcih vcedgsliaa ggsdgkvvvw qsaep
//
LOCUS       XP_077504121             378 aa            linear   INV 25-NOV-2025
DEFINITION  protein FAN-like [Amblyomma americanum].
ACCESSION   XP_077504121
VERSION     XP_077504121.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647995.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..378
                     /product="protein FAN-like"
                     /calculated_mol_wt=42411
     Region          <21..190
                     /region_name="Beach"
                     /note="Beige/BEACH domain; pfam02138"
                     /db_xref="CDD:460459"
     Region          256..311
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <273..374
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          316..350
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..378
                     /gene="LOC144114341"
                     /coded_by="XM_077647995.1:93..1229"
                     /db_xref="GeneID:144114341"
ORIGIN      
        1 mdgfwvvkev vlmnffkqvp qymlclqngr fdhpdrmfns isdtwrnvtt daadfkelip
       61 efydptcngd flvnslnldf gkhqngcavg dvelpqwaqg pahfirtcre alessfvsen
      121 lhdwidlifg ykqtgeeavl adnvyyhlty egsvdldsiq diyerhslev qimefgqtpk
      181 qifkhphpqr cctvlprsls masdscpdsl lenvssipee letrdrapsi ssfawkrnma
      241 dltkmaehml hnemvthvcf ssskssvfsv sqdsllklys leqkqqlrsv nishmalssc
      301 ivmndktvvv gswdnyvclc wdgatvltgc dsgsilvwdl lsaklvatvs ahsgpvtcih
      361 vkedgsdgkv vvwqsaep
//
LOCUS       XP_077504122             259 aa            linear   INV 25-NOV-2025
DEFINITION  RAS oncogene family member RabX6 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504122
VERSION     XP_077504122.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647996.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..259
                     /product="RAS oncogene family member RabX6 isoform X1"
                     /calculated_mol_wt=28761
     Region          65..226
                     /region_name="Rab"
                     /note="Ras-related in brain (Rab) family of small
                     guanosine triphosphatases (GTPases); cd00154"
                     /db_xref="CDD:206640"
     Site            70..77
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206640"
     Site            order(72..78,88,97,123,177..178,180,210..212)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206640"
     Site            78..88
                     /site_type="other"
                     /note="Rab subfamily motif 2 (RabSF2)"
                     /db_xref="CDD:206640"
     Site            order(88,97..103)
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206640"
     Site            97
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206640"
     Site            order(98,100..102,117,119,126..127,130,134,139)
                     /site_type="active"
                     /note="effector interaction site [active]"
                     /db_xref="CDD:206640"
     Site            order(98..99,101,119..120,127,131..133)
                     /site_type="active"
                     /note="GDI interaction site [active]"
                     /db_xref="CDD:206640"
     Site            98..102
                     /site_type="other"
                     /note="Rab family motif 1 (RabF1)"
                     /db_xref="CDD:206640"
     Site            order(99..106,113,115)
                     /site_type="other"
                     /note="GEF interaction site [polypeptide binding]"
                     /db_xref="CDD:206640"
     Site            115..119
                     /site_type="other"
                     /note="Rab family motif 2 (RabF2)"
                     /db_xref="CDD:206640"
     Site            120..123
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206640"
     Site            order(123,125..128,130..135)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206640"
     Site            order(126..128,130..131)
                     /site_type="other"
                     /note="Rab family motif 3 (RabF3)"
                     /db_xref="CDD:206640"
     Site            134..135
                     /site_type="other"
                     /note="Rab family motif 4 (RabF4)"
                     /db_xref="CDD:206640"
     Site            143..148
                     /site_type="other"
                     /note="Rab family motif 5 (RabF5)"
                     /db_xref="CDD:206640"
     Site            170..174
                     /site_type="other"
                     /note="Rab subfamily motif 3 (RabSF3)"
                     /db_xref="CDD:206640"
     Site            177..180
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206640"
     Site            210..212
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206640"
     Site            226
                     /site_type="other"
                     /note="Rab subfamily motif 4 (RabSF4)"
                     /db_xref="CDD:206640"
     CDS             1..259
                     /gene="RabX6"
                     /coded_by="XM_077647996.1:4..783"
                     /db_xref="GeneID:144114342"
ORIGIN      
        1 mlekactgll vvyyrppswl hsietsgdhi srsflflcsk nygsfsrgcr psfaamastk
       61 lpeqkvilcg eygvgksslf rrfttntfva sndrsstlgl dhfdkvykva grdlklqlwd
      121 tggmervasv tssyykfaea avlvfsldnp dsfnilsqhl ldivsyaena kiflcgnkvd
      181 lvgriyvsea dieafceqch nlisgvyrts cktgqgieem ftdiarqlvm scrvkdlddv
      241 akdsfkvtqp eetqescsc
//
LOCUS       XP_077504123             204 aa            linear   INV 25-NOV-2025
DEFINITION  RAS oncogene family member RabX6 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504123
VERSION     XP_077504123.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647997.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..204
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..204
                     /product="RAS oncogene family member RabX6 isoform X2"
                     /calculated_mol_wt=22591
     Region          10..171
                     /region_name="Rab"
                     /note="Ras-related in brain (Rab) family of small
                     guanosine triphosphatases (GTPases); cd00154"
                     /db_xref="CDD:206640"
     Site            15..22
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206640"
     Site            order(17..23,33,42,68,122..123,125,155..157)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206640"
     Site            23..33
                     /site_type="other"
                     /note="Rab subfamily motif 2 (RabSF2)"
                     /db_xref="CDD:206640"
     Site            order(33,42..48)
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206640"
     Site            42
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206640"
     Site            order(43,45..47,62,64,71..72,75,79,84)
                     /site_type="active"
                     /note="effector interaction site [active]"
                     /db_xref="CDD:206640"
     Site            order(43..44,46,64..65,72,76..78)
                     /site_type="active"
                     /note="GDI interaction site [active]"
                     /db_xref="CDD:206640"
     Site            43..47
                     /site_type="other"
                     /note="Rab family motif 1 (RabF1)"
                     /db_xref="CDD:206640"
     Site            order(44..51,58,60)
                     /site_type="other"
                     /note="GEF interaction site [polypeptide binding]"
                     /db_xref="CDD:206640"
     Site            60..64
                     /site_type="other"
                     /note="Rab family motif 2 (RabF2)"
                     /db_xref="CDD:206640"
     Site            65..68
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206640"
     Site            order(68,70..73,75..80)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206640"
     Site            order(71..73,75..76)
                     /site_type="other"
                     /note="Rab family motif 3 (RabF3)"
                     /db_xref="CDD:206640"
     Site            79..80
                     /site_type="other"
                     /note="Rab family motif 4 (RabF4)"
                     /db_xref="CDD:206640"
     Site            88..93
                     /site_type="other"
                     /note="Rab family motif 5 (RabF5)"
                     /db_xref="CDD:206640"
     Site            115..119
                     /site_type="other"
                     /note="Rab subfamily motif 3 (RabSF3)"
                     /db_xref="CDD:206640"
     Site            122..125
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206640"
     Site            155..157
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206640"
     Site            171
                     /site_type="other"
                     /note="Rab subfamily motif 4 (RabSF4)"
                     /db_xref="CDD:206640"
     CDS             1..204
                     /gene="RabX6"
                     /coded_by="XM_077647997.1:286..900"
                     /db_xref="GeneID:144114342"
ORIGIN      
        1 mastklpeqk vilcgeygvg ksslfrrftt ntfvasndrs stlgldhfdk vykvagrdlk
       61 lqlwdtggme rvasvtssyy kfaeaavlvf sldnpdsfni lsqhlldivs yaenakiflc
      121 gnkvdlvgri yvseadieaf ceqchnlisg vyrtscktgq gieemftdia rqlvmscrvk
      181 dlddvakdsf kvtqpeetqe scsc
//
LOCUS       XP_077504124             204 aa            linear   INV 25-NOV-2025
DEFINITION  RAS oncogene family member RabX6 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504124
VERSION     XP_077504124.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647998.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..204
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..204
                     /product="RAS oncogene family member RabX6 isoform X2"
                     /calculated_mol_wt=22591
     Region          10..171
                     /region_name="Rab"
                     /note="Ras-related in brain (Rab) family of small
                     guanosine triphosphatases (GTPases); cd00154"
                     /db_xref="CDD:206640"
     Site            15..22
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206640"
     Site            order(17..23,33,42,68,122..123,125,155..157)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206640"
     Site            23..33
                     /site_type="other"
                     /note="Rab subfamily motif 2 (RabSF2)"
                     /db_xref="CDD:206640"
     Site            order(33,42..48)
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206640"
     Site            42
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206640"
     Site            order(43,45..47,62,64,71..72,75,79,84)
                     /site_type="active"
                     /note="effector interaction site [active]"
                     /db_xref="CDD:206640"
     Site            order(43..44,46,64..65,72,76..78)
                     /site_type="active"
                     /note="GDI interaction site [active]"
                     /db_xref="CDD:206640"
     Site            43..47
                     /site_type="other"
                     /note="Rab family motif 1 (RabF1)"
                     /db_xref="CDD:206640"
     Site            order(44..51,58,60)
                     /site_type="other"
                     /note="GEF interaction site [polypeptide binding]"
                     /db_xref="CDD:206640"
     Site            60..64
                     /site_type="other"
                     /note="Rab family motif 2 (RabF2)"
                     /db_xref="CDD:206640"
     Site            65..68
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206640"
     Site            order(68,70..73,75..80)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206640"
     Site            order(71..73,75..76)
                     /site_type="other"
                     /note="Rab family motif 3 (RabF3)"
                     /db_xref="CDD:206640"
     Site            79..80
                     /site_type="other"
                     /note="Rab family motif 4 (RabF4)"
                     /db_xref="CDD:206640"
     Site            88..93
                     /site_type="other"
                     /note="Rab family motif 5 (RabF5)"
                     /db_xref="CDD:206640"
     Site            115..119
                     /site_type="other"
                     /note="Rab subfamily motif 3 (RabSF3)"
                     /db_xref="CDD:206640"
     Site            122..125
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206640"
     Site            155..157
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206640"
     Site            171
                     /site_type="other"
                     /note="Rab subfamily motif 4 (RabSF4)"
                     /db_xref="CDD:206640"
     CDS             1..204
                     /gene="RabX6"
                     /coded_by="XM_077647998.1:270..884"
                     /db_xref="GeneID:144114342"
ORIGIN      
        1 mastklpeqk vilcgeygvg ksslfrrftt ntfvasndrs stlgldhfdk vykvagrdlk
       61 lqlwdtggme rvasvtssyy kfaeaavlvf sldnpdsfni lsqhlldivs yaenakiflc
      121 gnkvdlvgri yvseadieaf ceqchnlisg vyrtscktgq gieemftdia rqlvmscrvk
      181 dlddvakdsf kvtqpeetqe scsc
//
LOCUS       XP_077504125             194 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114343 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504125
VERSION     XP_077504125.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077647999.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..194
                     /product="uncharacterized protein LOC144114343 isoform X1"
                     /calculated_mol_wt=21209
     Region          121..187
                     /region_name="EnY2"
                     /note="Transcription factor e(y)2; pfam10163"
                     /db_xref="CDD:462972"
     CDS             1..194
                     /gene="LOC144114343"
                     /coded_by="XM_077647999.1:140..724"
                     /db_xref="GeneID:144114343"
ORIGIN      
        1 msciancvna lltaslcllp lfhqlissvg fcpgtlrass cvfspqpdsn vvclraerlr
       61 rslpsvlarg tlasvpaqrs ifvcvflpqp icaamvlsse vfpveesdaa vqdpmksftr
      121 rlkellhtrl tecgwkdqlk ayckeiirek gvenisieel iaavtpiara tvpdiikrel
      181 vheiknfldq qacg
//
LOCUS       XP_077504126             132 aa            linear   INV 25-NOV-2025
DEFINITION  transcription and mRNA export factor ENY2-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077504126
VERSION     XP_077504126.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648000.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..132
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..132
                     /product="transcription and mRNA export factor ENY2-like
                     isoform X2"
                     /calculated_mol_wt=14647
     Region          60..125
                     /region_name="EnY2"
                     /note="Transcription factor e(y)2; pfam10163"
                     /db_xref="CDD:462972"
     CDS             1..132
                     /gene="LOC144114343"
                     /coded_by="XM_077648000.1:116..514"
                     /db_xref="GeneID:144114343"
ORIGIN      
        1 mcghrrstla yfaywlfsgm gcastallvv rscrsaprpf ysvvafttav caplfslpll
       61 kellhtrlte cgwkdqlkay ckeiirekgv enisieelia avtpiaratv pdiikrelvh
      121 eiknfldqqa cg
//
LOCUS       XP_077504127             145 aa            linear   INV 25-NOV-2025
DEFINITION  enhancer of yellow 2 transcription factor-like [Amblyomma
            americanum].
ACCESSION   XP_077504127
VERSION     XP_077504127.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648001.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..145
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..145
                     /product="enhancer of yellow 2 transcription factor-like"
                     /calculated_mol_wt=16173
     Region          71..137
                     /region_name="EnY2"
                     /note="Transcription factor e(y)2; pfam10163"
                     /db_xref="CDD:462972"
     CDS             1..145
                     /gene="LOC144114344"
                     /coded_by="XM_077648001.1:334..771"
                     /db_xref="GeneID:144114344"
ORIGIN      
        1 mlclraerlr rslpsvlarg tlasvpaqrs ifvcvflpqp icaamvlsse vfpveesdaa
       61 vqdpmksftr rlkellhtrl tecgwkdqlk ayckeiirek gvenvsveel iaavtprara
      121 tvpdiikrel vheiknflld qqacg
//
LOCUS       XP_077504128             119 aa            linear   INV 25-NOV-2025
DEFINITION  transcription and mRNA export factor ENY2-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077504128
VERSION     XP_077504128.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648002.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..119
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..119
                     /product="transcription and mRNA export factor ENY2-like
                     isoform X1"
                     /calculated_mol_wt=13585
     Region          33..111
                     /region_name="EnY2"
                     /note="Transcription factor e(y)2; pfam10163"
                     /db_xref="CDD:462972"
     CDS             1..119
                     /gene="LOC144114345"
                     /coded_by="XM_077648002.1:453..812"
                     /db_xref="GeneID:144114345"
ORIGIN      
        1 mvfnfaqrlp paakeeqagp lerqkvyqmk asinqklies gerdrlkell rtrliecgwr
       61 dqlkayckei irekgvenvs vdeliaavtp raratvpdii krellhqikn flidqqacg
//
LOCUS       XP_077504129             103 aa            linear   INV 25-NOV-2025
DEFINITION  transcription and mRNA export factor ENY2-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077504129
VERSION     XP_077504129.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648003.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..103
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..103
                     /product="transcription and mRNA export factor ENY2-like
                     isoform X2"
                     /calculated_mol_wt=11846
     Region          17..95
                     /region_name="EnY2"
                     /note="Transcription factor e(y)2; pfam10163"
                     /db_xref="CDD:462972"
     CDS             1..103
                     /gene="LOC144114345"
                     /coded_by="XM_077648003.1:161..472"
                     /db_xref="GeneID:144114345"
ORIGIN      
        1 mseplerqkv yqmkasinqk liesgerdrl kellrtrlie cgwrdqlkay ckeiirekgv
       61 envsvdelia avtpraratv pdiikrellh qiknflidqq acg
//
LOCUS       XP_077504130            2479 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114347 [Amblyomma americanum].
ACCESSION   XP_077504130
VERSION     XP_077504130.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648004.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2479
                     /product="uncharacterized protein LOC144114347"
                     /calculated_mol_wt=269233
     Region          299..411
                     /region_name="CUB"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; cd00041"
                     /db_xref="CDD:238001"
     Site            order(309,311,338,343,382,406,408,410..411)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     Region          415..540
                     /region_name="CUB"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; cd00041"
                     /db_xref="CDD:238001"
     Site            order(424,426,428,458,463,503,537,539)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     Region          563..704
                     /region_name="CLECT"
                     /note="C-type lectin (CTL)/C-type lectin-like (CTLD)
                     domain; cd00037"
                     /db_xref="CDD:153057"
     Site            order(665,669,671,688,690..693,696..698)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     Region          732..832
                     /region_name="CUB"
                     /note="Domain first found in C1r, C1s, uEGF, and bone
                     morphogenetic protein; smart00042"
                     /db_xref="CDD:214483"
     Region          857..989
                     /region_name="CLECT"
                     /note="C-type lectin (CTL)/C-type lectin-like (CTLD)
                     domain; cd00037"
                     /db_xref="CDD:153057"
     Site            order(956,960,967,973,975..978,981..983)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     Region          1002..1111
                     /region_name="CUB"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; cd00041"
                     /db_xref="CDD:238001"
     Site            order(1006,1008,1010,1037,1042,1080,1106,1108,1110..1111)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     Site            order(1132,1134,1136,1164,1169,1212,1242,1244,1246..1247)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     Region          1134..1245
                     /region_name="CUB"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; cl00049"
                     /db_xref="CDD:412131"
     Region          1381..1410
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     Region          1424..1452
                     /region_name="EGF_2"
                     /note="EGF-like domain; pfam07974"
                     /db_xref="CDD:400365"
     Region          1456..1491
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     CDS             1..2479
                     /gene="LOC144114347"
                     /coded_by="XM_077648004.1:669..8108"
                     /db_xref="GeneID:144114347"
ORIGIN      
        1 marapavsrl llaallaawr aalggvaeht asnateagge alfwppgdwq rqlkgcdkyv
       61 dgawngsarg pdssrfqlrh pflslvegvv pnvtyavilr pgaqleahsv lvaaqppgel
      121 valpkntyrc glngsfavly pvrdqpllqw rpmsgtrdgr ngsslltviy ssgtgaleve
      181 hlrfplrkec filyrahyav iqssdldkkq lecpkltllf eapedttpil qfqelrlsgs
      241 vaaqvevlae teaakglvlg nssvlrtsgr gqwlalrars crscyyravv yalnsregcn
      301 atltrtrgew hsphhpdfyp pglhcfitil apsgyrvllr ipflelppan eprcehdsle
      361 igplagrgpr svvlcgsrrn ltylslderl sitfrsdpll eaegwtvkye fvrlcrneth
      421 seasgrvcyp pgkstsllys apgdchfalv mplgyevqls fstlqvsaar assscpdnfl
      481 evssggtehr fcgnwtgsad lsalrsagnv mairvhleep pgsskpltls aaslgfcaty
      541 davlvnqsla edcefgwvsw glhcyrvfne slswakandv calqgghlas vttedlqall
      601 damlfssaey knhhhtfwig sndlqyencf twadgqefey thwfpgwssh ggygtqpsdd
      661 glaqqdcvev rdvflfpskg qgrtatfywn drhcavanpf vcqrlrhgat levgrtvdcn
      721 rtlvlspehs rgvvsspnyp gaypnsqhcs vdiqapagfl velrfsefll edhpgceydy
      781 veidepdegh ssgrlcgdws grvkllrqys hgrrlrvqft sdfshafkgf kaeavlrpea
      841 vptqlcdarq fqrfadhcyl tasfpevswa taqrvcsesq atlaavrtaa qeahlkalva
      901 ategsgsgvw ywlggrkvgq radagswvde asaenaadtt eeapgeavgp gakgtplcla
      961 lqwrpqhngs dlhwawkpcd hsgryicqkk aftlprdlns trtepsgtlt slhhpahyln
     1021 dlhysvsivg ppgsrvllsf srldlewqad clydyieiqs phgqadpvri cgqhetdldr
     1081 fdhfsesnel rltfhsdysv tatgfaaswt tvdmaslche ppptltaykd rpqslvsphy
     1141 plfypnsmhc ryviksetps lpvlvtledl evgvrdpsgh clgdrvsvrl sattahgspt
     1201 tvilcggeqs vprgyqllsy gdtlkltlea qqhrtrrhrg fnitfralpg pslettlqlp
     1261 arsrglvpsl nhphrppqgl aysvrllapl gqrvelrfvn rstasgaaat ihlwdpyrsp
     1321 qlalrlspsa dnatvtstlh tlrlsysapa apfqayfvtr kdplyldktv tlladvslds
     1381 ctpnpclngg sciedvtskk rlcsctppfa alfchvhacd vgdacsgrgn csvsptgalq
     1441 cscqqgfqgp dctqsvdpca glpcgpqgrc vavngsfvch cniffegprc ekqlfrlpyr
     1501 plsqrmleep fwlglitvsa vlllillvyc ikrkfadkie kflaeeiers knnpsppatr
     1561 yslsstqpsl tpaslspqpc qrsllsrirr hsirsaqggs spsdkeqgac tfsfdellkr
     1621 ssqssgskkq sanssdsdnp mpgtsgpgpa pkekesetsr ilaslvtssk spgkrrmsld
     1681 efiklserki qsikreetpg disdvsvase gsletsfiqr tsefhsireq sqeqnsssds
     1741 lsagpktsev qaevtplpes psrcsplgep lsrqdavasg gsrspsprsf veqqssslee
     1801 kakeapsata atttvaetla apsaepaspk aagdpkgafl dllqktmmaa spaafpkspt
     1861 kqqqqqqqqq qqqqqqqqqq lqqqqqpdtt qvsmlslpkv gsgckqkatd lsvprkysvd
     1921 lpmpkilita nmssceseea pspprtpnps cktmnylspl timcssadrt isesnlstsg
     1981 ysslsspgls rcnssspiad elenpahqgr rasvspksgh slspnymscg akafqfpvaq
     2041 aypyleahrp tspllqkrrs siggalcpat pslkdrvtlk gkyratrhsy qttstedsvd
     2101 degigidiae irmskscirg ggsvdiildt gkfdvperdv sratspfaks isfesprcrk
     2161 adkhrkgekh rrptpgsspg itpsseslpn ellaeaklsr npskssmaaa athstsdsdd
     2221 gelrprrpck sprggnarsp vdgraasplf lcqqhssssd slpstneepv gtdcsstdcs
     2281 adrgrpfsel aividsshca pgqtielipc lprlrvhplp qrreeatnts splpalrlis
     2341 peggtcrsld etaeprsprp srrsslspsg npstysages cnrtptsptp thhqilkrqe
     2401 alveddgsde svsettvllp rspkqpgsas prsggdstra rtrmgspfps sldsadggtq
     2461 ggrdgdated lydspgdvv
//
LOCUS       XP_077504131             402 aa            linear   INV 25-NOV-2025
DEFINITION  F-box/LRR-repeat protein 8-like [Amblyomma americanum].
ACCESSION   XP_077504131
VERSION     XP_077504131.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648005.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..402
                     /product="F-box/LRR-repeat protein 8-like"
                     /calculated_mol_wt=45917
     Region          19..66
                     /region_name="F-box_SF"
                     /note="F-box domain superfamily; cl45894"
                     /db_xref="CDD:459239"
     Site            order(22..23,25..27,30..31,34,45..47,49,52)
                     /site_type="other"
                     /note="F-box motif"
                     /db_xref="CDD:438852"
     Site            order(22,26,29..30,33..34,38,40..41,45..47,49)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438852"
     Region          76..>280
                     /region_name="FBXL3_LRR-like"
                     /note="Leucine-rich repeat domain of FBXL3 and related
                     proteins; cl45928"
                     /db_xref="CDD:480268"
     Region          153..179
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          180..208
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          209..234
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     CDS             1..402
                     /gene="LOC144114348"
                     /coded_by="XM_077648005.1:190..1398"
                     /db_xref="GeneID:144114348"
ORIGIN      
        1 mcyfhyivtm eddhkivsye slpeaillei fmklslpdrl qaalscrrwc niftspaawt
       61 nfhfrfygrk dtrllqciis ygcylrqvvi elnqfqnvnr vnavqallll akcsstrlsh
      121 lriictgenp lfyagkefvs aleavfkrcn shlvevdlgk fpmmidshti dvlsqhspql
      181 eclniqnnnl vclvqpeciv rlaqrcrrlr dlrvhkcsls qdvllcfleq rrvglehlsl
      241 qcrseeiiat hpqdektqls selwsrvvsg lprlrvtlhf dqtcplvvip lvmrpevpca
      301 vlrletftii hdmvllaays yaetleklil qappsaelec allevsrrcs klralhvfcp
      361 lseqtidtil wehpcmkasg lytlrseags dgpwatdekv da
//
LOCUS       XP_077504132             186 aa            linear   INV 25-NOV-2025
DEFINITION  cancer-related nucleoside-triphosphatase homolog [Amblyomma
            americanum].
ACCESSION   XP_077504132
VERSION     XP_077504132.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648006.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..186
                     /product="cancer-related nucleoside-triphosphatase
                     homolog"
                     /calculated_mol_wt=20184
     Region          7..177
                     /region_name="NTPase_1"
                     /note="pfam03266"
                     /db_xref="CDD:460869"
     CDS             1..186
                     /gene="LOC144114349"
                     /coded_by="XM_077648006.1:130..690"
                     /db_xref="GeneID:144114349"
ORIGIN      
        1 maspkfivlt gspgvgktti vknavktlge qgisvsgfyt telrengnrv gfdvvttgnk
       61 kaplarirdh fpagkgpfvg rycvtvktfe evaldslktk sdvlvidevg kmelfsepfr
      121 nrvselfade nncilvtvpv stgtqclplv dhikghkcar vfsvnrsnrd ylvkevldal
      181 rkavgk
//
LOCUS       XP_077504133             182 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin-conjugating enzyme E2 C-like [Amblyomma americanum].
ACCESSION   XP_077504133
VERSION     XP_077504133.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648007.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..182
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..182
                     /product="ubiquitin-conjugating enzyme E2 C-like"
                     /calculated_mol_wt=20572
     Region          32..171
                     /region_name="UBCc_UBE2C"
                     /note="Ubiquitin-conjugating enzyme E2, catalytic (UBCc)
                     domain of ubiquitin-conjugating enzyme E2 C and related
                     proteins; cd23791"
                     /db_xref="CDD:467411"
     CDS             1..182
                     /gene="LOC144114350"
                     /coded_by="XM_077648007.1:107..655"
                     /db_xref="GeneID:144114350"
ORIGIN      
        1 masqnvdpla qdeyvkssde qrsvdsgrhs vtkrlqqelm tlmmcgekgi safpdgdnlf
       61 rwigtihgps gtvyeklvyr lrlefpstyp fespqvhfet pcfhpnvdaq gnicldilke
      121 kwtalldvrt vllsiqslla epnvssplng yaaeiwsnqv lykkvlleky ekqskeeprn
      181 vp
//
LOCUS       XP_077504134             351 aa            linear   INV 25-NOV-2025
DEFINITION  putative ribosome-binding factor A, mitochondrial isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077504134
VERSION     XP_077504134.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648008.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..351
                     /product="putative ribosome-binding factor A,
                     mitochondrial isoform X1"
                     /calculated_mol_wt=39473
     Region          76..182
                     /region_name="RBFA"
                     /note="Ribosome-binding factor A; pfam02033"
                     /db_xref="CDD:460422"
     CDS             1..351
                     /gene="LOC144114351"
                     /coded_by="XM_077648008.1:45..1100"
                     /db_xref="GeneID:144114351"
ORIGIN      
        1 maelpgllrr faetrslfpf trrhfsvchr dnaklgvflh klidkskkrh pqqyvysapn
       61 savpsaprkv qertrrqrvl navfmenisn imvtgdisek lrgcavqltq vrisadcthl
      121 nvywvtslqe hgadervael lqsnagflks eliksgfigk apkitfvkdv tygrgaever
      181 lidkigadip pdldlpestd eqfldsesat esvstsaadv psrpyvpsdm kvdiygldra
      241 aimrqvllkt kglgrtplee ptflkpgten lqetqarmls laeyvqqrkl ksrlqikaer
      301 eaqrslsgvq gnldekhtle nasypevdye vdideevedl snvsfldeqe r
//
LOCUS       XP_077504135             317 aa            linear   INV 25-NOV-2025
DEFINITION  putative ribosome-binding factor A, mitochondrial isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077504135
VERSION     XP_077504135.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648009.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..317
                     /product="putative ribosome-binding factor A,
                     mitochondrial isoform X2"
                     /calculated_mol_wt=35537
     Region          42..148
                     /region_name="RBFA"
                     /note="Ribosome-binding factor A; pfam02033"
                     /db_xref="CDD:460422"
     CDS             1..317
                     /gene="LOC144114351"
                     /coded_by="XM_077648009.1:129..1082"
                     /db_xref="GeneID:144114351"
ORIGIN      
        1 mdfehktdsl lrikrhpqqy vysapnsavp saprkvqert rrqrvlnavf menisnimvt
       61 gdiseklrgc avqltqvris adcthlnvyw vtslqehgad ervaellqsn agflkselik
      121 sgfigkapki tfvkdvtygr gaeverlidk igadippdld lpestdeqfl dsesatesvs
      181 tsaadvpsrp yvpsdmkvdi ygldraaimr qvllktkglg rtpleeptfl kpgtenlqet
      241 qarmlslaey vqqrklksrl qikaereaqr slsgvqgnld ekhtlenasy pevdyevdid
      301 eevedlsnvs fldeqer
//
LOCUS       XP_077504136             222 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase RNF166-like [Amblyomma americanum].
ACCESSION   XP_077504136
VERSION     XP_077504136.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648010.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..222
                     /product="E3 ubiquitin-protein ligase RNF166-like"
                     /calculated_mol_wt=24469
     Region          18..>64
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(23,26,38,40,43,46,57,60)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438259"
     Region          135..194
                     /region_name="zf-Di19"
                     /note="Drought induced 19 protein (Di19), zinc-binding;
                     pfam05605"
                     /db_xref="CDD:428539"
     CDS             1..222
                     /gene="LOC144114352"
                     /coded_by="XM_077648010.1:129..797"
                     /db_xref="GeneID:144114352"
ORIGIN      
        1 mmarlslfka tssmtnntee ficpicleif qkpvtiscgh tfcsgclaqc kqddpkcplc
       61 rstfdpsktv rakdiaksis tlkapcsgcr kvlslsklra hhsncsdele gaaaappvyq
      121 pprgqssgke nrciftcpyc eadnldlaal rdhcnanhyn npqsvvcpvc asmpwgnphq
      181 ksinflshln lrhrfeydty vdygmdddda lqqaleaslk ek
//
LOCUS       XP_077504137             222 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase RNF166-like [Amblyomma americanum].
ACCESSION   XP_077504137
VERSION     XP_077504137.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648011.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..222
                     /product="E3 ubiquitin-protein ligase RNF166-like"
                     /calculated_mol_wt=24469
     Region          18..>64
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(23,26,38,40,43,46,57,60)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438259"
     Region          135..194
                     /region_name="zf-Di19"
                     /note="Drought induced 19 protein (Di19), zinc-binding;
                     pfam05605"
                     /db_xref="CDD:428539"
     CDS             1..222
                     /gene="LOC144114352"
                     /coded_by="XM_077648011.1:129..797"
                     /db_xref="GeneID:144114352"
ORIGIN      
        1 mmarlslfka tssmtnntee ficpicleif qkpvtiscgh tfcsgclaqc kqddpkcplc
       61 rstfdpsktv rakdiaksis tlkapcsgcr kvlslsklra hhsncsdele gaaaappvyq
      121 pprgqssgke nrciftcpyc eadnldlaal rdhcnanhyn npqsvvcpvc asmpwgnphq
      181 ksinflshln lrhrfeydty vdygmdddda lqqaleaslk ek
//
LOCUS       XP_077504138             188 aa            linear   INV 25-NOV-2025
DEFINITION  programmed cell death protein 6-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504138
VERSION     XP_077504138.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648012.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..188
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..188
                     /product="programmed cell death protein 6-like isoform X1"
                     /calculated_mol_wt=21575
     Region          16..187
                     /region_name="EFh_PEF"
                     /note="The penta-EF hand (PEF) family; cl25352"
                     /db_xref="CDD:355382"
     Region          16..45
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320054"
     Site            order(29,36,69,71,73,80,99,101,103,110)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320054"
     Site            order(53,55,72,126,129,132..135,146,150,153..154,157..158,
                     161,170..177,179..185)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:320054"
     Region          53..89
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320054"
     Region          90..119
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320054"
     Region          126..155
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320054"
     Region          157..185
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320054"
     CDS             1..188
                     /gene="LOC144114354"
                     /coded_by="XM_077648012.1:233..799"
                     /db_xref="GeneID:144114354"
ORIGIN      
        1 myshappqap kadvnylqgl frnvdkdrsg nisaaelqsa lsngtwkpfn petvrmmidm
       61 frsspgmfdr hrtgtvnfde fvslwnyitn wlncfqsfdk drsgnidkde lsealtkfgy
      121 rlsepimsml lvkfdrdgkg sinfddfiqc cvtlqtltaa frhydtdqdg witigyedfl
      181 klvfslrm
//
LOCUS       XP_077504139             181 aa            linear   INV 25-NOV-2025
DEFINITION  programmed cell death protein 6-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504139
VERSION     XP_077504139.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648013.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="programmed cell death protein 6-like isoform X2"
                     /calculated_mol_wt=20755
     Region          16..180
                     /region_name="EFh_PEF_ALG-2"
                     /note="EF-hand, calcium binding motif, found in
                     apoptosis-linked gene 2 protein (ALG-2) and similar
                     proteins; cd16183"
                     /db_xref="CDD:320058"
     Region          16..45
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320058"
     Site            order(25,27,29,36,92,94,96,103,158,160,162)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320058"
     Region          53..82
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320058"
     Site            order(60..61,63,80,83..84,111..112,148,151,154..155,158,
                     161,163)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:320058"
     Region          83..113
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320058"
     Site            order(113,119,122..123,126,147,150..151,153..154,163..170,
                     172..173,176..177,180)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:320058"
     Region          119..148
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320058"
     Region          149..180
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320058"
     CDS             1..181
                     /gene="LOC144114354"
                     /coded_by="XM_077648013.1:232..777"
                     /db_xref="GeneID:144114354"
ORIGIN      
        1 myshappqap kadvnylqgl frnvdkdrsg nisaaelqsa lsngtwkpfn petvrmmigm
       61 fdrhrtgtvn fdefvslwny itnwlncfqs fdkdrsgnid kdelsealtk fgyrlsepim
      121 smllvkfdrd gkgsinfddf iqccvtlqtl taafrhydtd qdgwitigye dflklvfslr
      181 m
//
LOCUS       XP_077504140             164 aa            linear   INV 25-NOV-2025
DEFINITION  programmed cell death protein 6-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504140
VERSION     XP_077504140.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648014.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..164
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..164
                     /product="programmed cell death protein 6-like isoform X3"
                     /calculated_mol_wt=19280
     Region          21..163
                     /region_name="EFh_PEF"
                     /note="The penta-EF hand (PEF) family; cl25352"
                     /db_xref="CDD:355382"
     Site            order(29,31,48,102,105,108..111,122,126,129..130,133..134,
                     137,146..153,155..161)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:320029"
     Region          29..65
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320029"
     Site            order(45,47,49,56,75,77,79,86)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320029"
     Region          66..95
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320029"
     Region          102..131
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320029"
     Region          133..161
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320029"
     CDS             1..164
                     /gene="LOC144114354"
                     /coded_by="XM_077648014.1:246..740"
                     /db_xref="GeneID:144114354"
ORIGIN      
        1 mprlkpqrlt stiyrgfser twkpfnpetv rmmidmfrss pgmfdrhrtg tvnfdefvsl
       61 wnyitnwlnc fqsfdkdrsg nidkdelsea ltkfgyrlse pimsmllvkf drdgkgsinf
      121 ddfiqccvtl qtltaafrhy dtdqdgwiti gyedflklvf slrm
//
LOCUS       XP_077504142             157 aa            linear   INV 25-NOV-2025
DEFINITION  programmed cell death protein 6-like isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077504142
VERSION     XP_077504142.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648016.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..157
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..157
                     /product="programmed cell death protein 6-like isoform X4"
                     /calculated_mol_wt=18459
     Region          21..156
                     /region_name="EFh_PEF"
                     /note="The penta-EF hand (PEF) family; cl25352"
                     /db_xref="CDD:355382"
     Site            order(29,31,41,95,98,101..104,115,119,122..123,126..127,
                     130,139..146,148..154)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:320054"
     Region          29..58
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320054"
     Site            order(38,40,42,49,68,70,72,79)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320054"
     Region          59..88
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320054"
     Region          95..124
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320054"
     Region          126..154
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320054"
     CDS             1..157
                     /gene="LOC144114354"
                     /coded_by="XM_077648016.1:245..718"
                     /db_xref="GeneID:144114354"
ORIGIN      
        1 mprlkpqrlt stiyrgfser twkpfnpetv rmmigmfdrh rtgtvnfdef vslwnyitnw
       61 lncfqsfdkd rsgnidkdel sealtkfgyr lsepimsmll vkfdrdgkgs infddfiqcc
      121 vtlqtltaaf rhydtdqdgw itigyedflk lvfslrm
//
LOCUS       XP_077504143             371 aa            linear   INV 25-NOV-2025
DEFINITION  GMP reductase 2-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504143
VERSION     XP_077504143.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648017.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..371
                     /product="GMP reductase 2-like isoform X1"
                     /calculated_mol_wt=40121
     Region          3..367
                     /region_name="PRK05096"
                     /note="guanosine 5'-monophosphate oxidoreductase;
                     Provisional"
                     /db_xref="CDD:235343"
     CDS             1..371
                     /gene="LOC144114355"
                     /coded_by="XM_077648017.1:139..1254"
                     /db_xref="GeneID:144114355"
ORIGIN      
        1 mphidndvkl dfkdvllrpk rstlksriev slmsigavrv ckrnflfcll lqvdlnrtfk
       61 frnskatytg ipiiaanmdt vgtfemarvl akhslftaih khysveqwke fcasnpsise
      121 nvavssgsge adygkvkail reipalryic ldvangysea fvqfvrsvra dfpthtimag
      181 nvvtgemvee lllsgadiik vgigpgsvct trkktgvgyp qlsaviecad aahgldghii
      241 sdggctcpgd vskafgagad fvmlggmlag hdesggevie kdgkmykmfy gmssatamek
      301 yhggvaeyra segktvqvpy rgkvqatvld ilgglrsact ytgagrlkel trrttfirvt
      361 qqlnevfcke a
//
LOCUS       XP_077504144             348 aa            linear   INV 25-NOV-2025
DEFINITION  GMP reductase 2-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504144
VERSION     XP_077504144.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648018.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..348
                     /product="GMP reductase 2-like isoform X2"
                     /calculated_mol_wt=37528
     Region          3..344
                     /region_name="PRK05096"
                     /note="guanosine 5'-monophosphate oxidoreductase;
                     Provisional"
                     /db_xref="CDD:235343"
     CDS             1..348
                     /gene="LOC144114355"
                     /coded_by="XM_077648018.1:299..1345"
                     /db_xref="GeneID:144114355"
ORIGIN      
        1 mphidndvkl dfkdvllrpk rstlksriev dlnrtfkfrn skatytgipi iaanmdtvgt
       61 femarvlakh slftaihkhy sveqwkefca snpsisenva vssgsgeady gkvkailrei
      121 palryicldv angyseafvq fvrsvradfp thtimagnvv tgemveelll sgadiikvgi
      181 gpgsvcttrk ktgvgypqls aviecadaah gldghiisdg gctcpgdvsk afgagadfvm
      241 lggmlaghde sggeviekdg kmykmfygms satamekyhg gvaeyraseg ktvqvpyrgk
      301 vqatvldilg glrsactytg agrlkeltrr ttfirvtqql nevfckea
//
LOCUS       XP_077504145             702 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114356 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504145
VERSION     XP_077504145.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648019.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..702
                     /product="uncharacterized protein LOC144114356 isoform X1"
                     /calculated_mol_wt=76154
     Region          14..112
                     /region_name="HSF_DNA-bind"
                     /note="HSF-type DNA-binding; pfam00447"
                     /db_xref="CDD:459813"
     Region          <146..179
                     /region_name="BRLZ"
                     /note="basic region leucin zipper; smart00338"
                     /db_xref="CDD:197664"
     Region          239..>360
                     /region_name="Vert_HS_TF"
                     /note="Vertebrate heat shock transcription factor;
                     pfam06546"
                     /db_xref="CDD:461944"
     CDS             1..702
                     /gene="LOC144114356"
                     /coded_by="XM_077648019.1:112..2220"
                     /db_xref="GeneID:144114356"
ORIGIN      
        1 mhttevgvsn vpaflvklwk lvedekcndl iswssngqsf iihnqtqfak ellplyfkhn
       61 nmasfirqln mygfrkvtni dqglrsdree ieffhnffvr gqecllefik rkvpsgragg
      121 vgpddgrarn evlkellsnv gsmqgrqehm dqlladmkke nealwrevar lrqkhmkqqq
      181 ivekliqflv tmvqanrnit vkrkmplmlh dspnssskvp rltkgtfvtd yqvtspagsq
      241 msegpvirdv tdlmddfget sssvvsepma agqsprvvev saagtpvrsl aspaassdld
      301 agpivaspls tadvmeplcl ldplegdqss dalpcdilev vvdssdsspk epvaacskdl
      361 vtpavkaaqm tpatssvmqi tpaksvtpas pliseekpam lgdkqaangt sdlldscill
      421 edlplfagdt tppvaaspsg lpatstdvat eetsghnkva ttppanmemq valqdktagt
      481 trvfadhves idsdldwlhd qlmsgglsld tstlmgvcpd wtglflgspk lfspedplts
      541 rlgytgnrii hlsceqsrcp ssqwplkfsr aiqvhcyhta nfkdhsdlat enrcssdavg
      601 nevvqytpsl tdlgieedps fqpvhellls ddelpenssa tnvtngalvs sltttkenps
      661 rrsispsvly pddlkavvqt gsrlvgkkgs tfgkkrrgns rk
//
LOCUS       XP_077504146             688 aa            linear   INV 25-NOV-2025
DEFINITION  heat shock factor protein 1-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504146
VERSION     XP_077504146.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648020.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..688
                     /product="heat shock factor protein 1-like isoform X2"
                     /calculated_mol_wt=74653
     Region          14..112
                     /region_name="HSF_DNA-bind"
                     /note="HSF-type DNA-binding; pfam00447"
                     /db_xref="CDD:459813"
     Region          <146..179
                     /region_name="BRLZ"
                     /note="basic region leucin zipper; smart00338"
                     /db_xref="CDD:197664"
     Region          239..>360
                     /region_name="Vert_HS_TF"
                     /note="Vertebrate heat shock transcription factor;
                     pfam06546"
                     /db_xref="CDD:461944"
     Region          <432..>520
                     /region_name="Vert_HS_TF"
                     /note="Vertebrate heat shock transcription factor;
                     pfam06546"
                     /db_xref="CDD:461944"
     CDS             1..688
                     /gene="LOC144114356"
                     /coded_by="XM_077648020.1:112..2178"
                     /db_xref="GeneID:144114356"
ORIGIN      
        1 mhttevgvsn vpaflvklwk lvedekcndl iswssngqsf iihnqtqfak ellplyfkhn
       61 nmasfirqln mygfrkvtni dqglrsdree ieffhnffvr gqecllefik rkvpsgragg
      121 vgpddgrarn evlkellsnv gsmqgrqehm dqlladmkke nealwrevar lrqkhmkqqq
      181 ivekliqflv tmvqanrnit vkrkmplmlh dspnssskvp rltkgtfvtd yqvtspagsq
      241 msegpvirdv tdlmddfget sssvvsepma agqsprvvev saagtpvrsl aspaassdld
      301 agpivaspls tadvmeplcl ldplegdqss dalpcdilev vvdssdsspk epvaacskdl
      361 vtpavkaaqm tpatssvmqi tpaksvtpas pliseekpam lgdkqaangt sdlldscill
      421 edlplfagdt tppvaaspsg lpatstdvat eetsghnkva ttppanmemq valqdktagt
      481 trvfadhves idsdldwlhd qlmsgglsld tstlmglfsp edpltsrlgy tgnriihlsc
      541 eqsrcpssqw plkfsraiqv hcyhtanfkd hsdlatenrc ssdavgnevv qytpsltdlg
      601 ieedpsfqpv helllsddel penssatnvt ngalvssltt tkenpsrrsi spsvlypddl
      661 kavvqtgsrl vgkkgstfgk krrgnsrk
//
LOCUS       XP_077504147             679 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114356 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504147
VERSION     XP_077504147.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648021.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..679
                     /product="uncharacterized protein LOC144114356 isoform X3"
                     /calculated_mol_wt=73682
     Region          13..>173
                     /region_name="HSF1"
                     /note="Heat shock transcription factor [Transcription];
                     COG5169"
                     /db_xref="CDD:227497"
     Region          <13..89
                     /region_name="HSF_DNA-bind"
                     /note="HSF-type DNA-binding; pfam00447"
                     /db_xref="CDD:459813"
     Region          216..>337
                     /region_name="Vert_HS_TF"
                     /note="Vertebrate heat shock transcription factor;
                     pfam06546"
                     /db_xref="CDD:461944"
     CDS             1..679
                     /gene="LOC144114356"
                     /coded_by="XM_077648021.1:41..2080"
                     /db_xref="GeneID:144114356"
ORIGIN      
        1 mdhrqhqlaq qlngqsfiih nqtqfakell plyfkhnnma sfirqlnmyg frkvtnidqg
       61 lrsdreeief fhnffvrgqe cllefikrkv psgraggvgp ddgrarnevl kellsnvgsm
      121 qgrqehmdql ladmkkenea lwrevarlrq khmkqqqive kliqflvtmv qanrnitvkr
      181 kmplmlhdsp nssskvprlt kgtfvtdyqv tspagsqmse gpvirdvtdl mddfgetsss
      241 vvsepmaagq sprvvevsaa gtpvrslasp aassdldagp ivasplstad vmeplclldp
      301 legdqssdal pcdilevvvd ssdsspkepv aacskdlvtp avkaaqmtpa tssvmqitpa
      361 ksvtpaspli seekpamlgd kqaangtsdl ldscilledl plfagdttpp vaaspsglpa
      421 tstdvateet sghnkvattp panmemqval qdktagttrv fadhvesids dldwlhdqlm
      481 sgglsldtst lmgvcpdwtg lflgspklfs pedpltsrlg ytgnriihls ceqsrcpssq
      541 wplkfsraiq vhcyhtanfk dhsdlatenr cssdavgnev vqytpsltdl gieedpsfqp
      601 vhelllsdde lpenssatnv tngalvsslt ttkenpsrrs ispsvlypdd lkavvqtgsr
      661 lvgkkgstfg kkrrgnsrk
//
LOCUS       XP_077504148             659 aa            linear   INV 25-NOV-2025
DEFINITION  heat shock factor protein 1-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077504148
VERSION     XP_077504148.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648022.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..659
                     /product="heat shock factor protein 1-like isoform X4"
                     /calculated_mol_wt=71125
     Region          14..112
                     /region_name="HSF_DNA-bind"
                     /note="HSF-type DNA-binding; pfam00447"
                     /db_xref="CDD:459813"
     Region          <146..179
                     /region_name="BRLZ"
                     /note="basic region leucin zipper; smart00338"
                     /db_xref="CDD:197664"
     Region          239..>360
                     /region_name="Vert_HS_TF"
                     /note="Vertebrate heat shock transcription factor;
                     pfam06546"
                     /db_xref="CDD:461944"
     Region          <442..596
                     /region_name="Vert_HS_TF"
                     /note="Vertebrate heat shock transcription factor;
                     pfam06546"
                     /db_xref="CDD:461944"
     CDS             1..659
                     /gene="LOC144114356"
                     /coded_by="XM_077648022.1:112..2091"
                     /db_xref="GeneID:144114356"
ORIGIN      
        1 mhttevgvsn vpaflvklwk lvedekcndl iswssngqsf iihnqtqfak ellplyfkhn
       61 nmasfirqln mygfrkvtni dqglrsdree ieffhnffvr gqecllefik rkvpsgragg
      121 vgpddgrarn evlkellsnv gsmqgrqehm dqlladmkke nealwrevar lrqkhmkqqq
      181 ivekliqflv tmvqanrnit vkrkmplmlh dspnssskvp rltkgtfvtd yqvtspagsq
      241 msegpvirdv tdlmddfget sssvvsepma agqsprvvev saagtpvrsl aspaassdld
      301 agpivaspls tadvmeplcl ldplegdqss dalpcdilev vvdssdsspk epvaacskdl
      361 vtpavkaaqm tpatssvmqi tpaksvtpas pliseekpam lgdkqaangt sdlldscill
      421 edlplfagdt tppvaaspsg lpatstdvat eetsghnkva ttppanmemq valqdktagt
      481 trvfadhves idsdldwlhd qlmsgglsld tstlmgvcpd wtglflgspk lfspedplts
      541 rlgdlatenr cssdavgnev vqytpsltdl gieedpsfqp vhelllsdde lpenssatnv
      601 tngalvsslt ttkenpsrrs ispsvlypdd lkavvqtgsr lvgkkgstfg kkrrgnsrk
//
LOCUS       XP_077504149             645 aa            linear   INV 25-NOV-2025
DEFINITION  heat shock factor protein 1-like isoform X5 [Amblyomma americanum].
ACCESSION   XP_077504149
VERSION     XP_077504149.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648023.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..645
                     /product="heat shock factor protein 1-like isoform X5"
                     /calculated_mol_wt=69623
     Region          14..112
                     /region_name="HSF_DNA-bind"
                     /note="HSF-type DNA-binding; pfam00447"
                     /db_xref="CDD:459813"
     Region          <146..179
                     /region_name="BRLZ"
                     /note="basic region leucin zipper; smart00338"
                     /db_xref="CDD:197664"
     Region          <442..582
                     /region_name="Vert_HS_TF"
                     /note="Vertebrate heat shock transcription factor;
                     pfam06546"
                     /db_xref="CDD:461944"
     CDS             1..645
                     /gene="LOC144114356"
                     /coded_by="XM_077648023.1:112..2049"
                     /db_xref="GeneID:144114356"
ORIGIN      
        1 mhttevgvsn vpaflvklwk lvedekcndl iswssngqsf iihnqtqfak ellplyfkhn
       61 nmasfirqln mygfrkvtni dqglrsdree ieffhnffvr gqecllefik rkvpsgragg
      121 vgpddgrarn evlkellsnv gsmqgrqehm dqlladmkke nealwrevar lrqkhmkqqq
      181 ivekliqflv tmvqanrnit vkrkmplmlh dspnssskvp rltkgtfvtd yqvtspagsq
      241 msegpvirdv tdlmddfget sssvvsepma agqsprvvev saagtpvrsl aspaassdld
      301 agpivaspls tadvmeplcl ldplegdqss dalpcdilev vvdssdsspk epvaacskdl
      361 vtpavkaaqm tpatssvmqi tpaksvtpas pliseekpam lgdkqaangt sdlldscill
      421 edlplfagdt tppvaaspsg lpatstdvat eetsghnkva ttppanmemq valqdktagt
      481 trvfadhves idsdldwlhd qlmsgglsld tstlmglfsp edpltsrlgd latenrcssd
      541 avgnevvqyt psltdlgiee dpsfqpvhel llsddelpen ssatnvtnga lvssltttke
      601 npsrrsisps vlypddlkav vqtgsrlvgk kgstfgkkrr gnsrk
//
LOCUS       XP_077504150             636 aa            linear   INV 25-NOV-2025
DEFINITION  heat shock factor protein 1-like isoform X6 [Amblyomma americanum].
ACCESSION   XP_077504150
VERSION     XP_077504150.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648024.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..636
                     /product="heat shock factor protein 1-like isoform X6"
                     /calculated_mol_wt=68653
     Region          13..>173
                     /region_name="HSF1"
                     /note="Heat shock transcription factor [Transcription];
                     COG5169"
                     /db_xref="CDD:227497"
     Region          <13..89
                     /region_name="HSF_DNA-bind"
                     /note="HSF-type DNA-binding; pfam00447"
                     /db_xref="CDD:459813"
     Region          216..>337
                     /region_name="Vert_HS_TF"
                     /note="Vertebrate heat shock transcription factor;
                     pfam06546"
                     /db_xref="CDD:461944"
     Region          <419..573
                     /region_name="Vert_HS_TF"
                     /note="Vertebrate heat shock transcription factor;
                     pfam06546"
                     /db_xref="CDD:461944"
     CDS             1..636
                     /gene="LOC144114356"
                     /coded_by="XM_077648024.1:56..1966"
                     /db_xref="GeneID:144114356"
ORIGIN      
        1 mdhrqhqlaq qlngqsfiih nqtqfakell plyfkhnnma sfirqlnmyg frkvtnidqg
       61 lrsdreeief fhnffvrgqe cllefikrkv psgraggvgp ddgrarnevl kellsnvgsm
      121 qgrqehmdql ladmkkenea lwrevarlrq khmkqqqive kliqflvtmv qanrnitvkr
      181 kmplmlhdsp nssskvprlt kgtfvtdyqv tspagsqmse gpvirdvtdl mddfgetsss
      241 vvsepmaagq sprvvevsaa gtpvrslasp aassdldagp ivasplstad vmeplclldp
      301 legdqssdal pcdilevvvd ssdsspkepv aacskdlvtp avkaaqmtpa tssvmqitpa
      361 ksvtpaspli seekpamlgd kqaangtsdl ldscilledl plfagdttpp vaaspsglpa
      421 tstdvateet sghnkvattp panmemqval qdktagttrv fadhvesids dldwlhdqlm
      481 sgglsldtst lmgvcpdwtg lflgspklfs pedpltsrlg dlatenrcss davgnevvqy
      541 tpsltdlgie edpsfqpvhe lllsddelpe nssatnvtng alvssltttk enpsrrsisp
      601 svlypddlka vvqtgsrlvg kkgstfgkkr rgnsrk
//
LOCUS       XP_077504151             566 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114356 isoform X7 [Amblyomma
            americanum].
ACCESSION   XP_077504151
VERSION     XP_077504151.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648025.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..566
                     /product="uncharacterized protein LOC144114356 isoform X7"
                     /calculated_mol_wt=61362
     Region          14..112
                     /region_name="HSF_DNA-bind"
                     /note="HSF-type DNA-binding; pfam00447"
                     /db_xref="CDD:459813"
     Region          <146..179
                     /region_name="BRLZ"
                     /note="basic region leucin zipper; smart00338"
                     /db_xref="CDD:197664"
     Region          239..>360
                     /region_name="Vert_HS_TF"
                     /note="Vertebrate heat shock transcription factor;
                     pfam06546"
                     /db_xref="CDD:461944"
     Region          <432..>552
                     /region_name="Vert_HS_TF"
                     /note="Vertebrate heat shock transcription factor;
                     pfam06546"
                     /db_xref="CDD:461944"
     CDS             1..566
                     /gene="LOC144114356"
                     /coded_by="XM_077648025.1:112..1812"
                     /db_xref="GeneID:144114356"
ORIGIN      
        1 mhttevgvsn vpaflvklwk lvedekcndl iswssngqsf iihnqtqfak ellplyfkhn
       61 nmasfirqln mygfrkvtni dqglrsdree ieffhnffvr gqecllefik rkvpsgragg
      121 vgpddgrarn evlkellsnv gsmqgrqehm dqlladmkke nealwrevar lrqkhmkqqq
      181 ivekliqflv tmvqanrnit vkrkmplmlh dspnssskvp rltkgtfvtd yqvtspagsq
      241 msegpvirdv tdlmddfget sssvvsepma agqsprvvev saagtpvrsl aspaassdld
      301 agpivaspls tadvmeplcl ldplegdqss dalpcdilev vvdssdsspk epvaacskdl
      361 vtpavkaaqm tpatssvmqi tpaksvtpas pliseekpam lgdkqaangt sdlldscill
      421 edlplfagdt tppvaaspsg lpatstdvat eetsghnkva ttppanmemq valqdktagt
      481 trvfadhves idsdldwlhd qlmsgglsld tstlmgvcpd wtglflgspk lfspedplts
      541 rlgvstdpgs qlctfpflkn qrsrwf
//
LOCUS       XP_077504152             552 aa            linear   INV 25-NOV-2025
DEFINITION  heat shock factor protein 1-like isoform X8 [Amblyomma americanum].
ACCESSION   XP_077504152
VERSION     XP_077504152.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648026.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..552
                     /product="heat shock factor protein 1-like isoform X8"
                     /calculated_mol_wt=59860
     Region          14..112
                     /region_name="HSF_DNA-bind"
                     /note="HSF-type DNA-binding; pfam00447"
                     /db_xref="CDD:459813"
     Region          <146..179
                     /region_name="BRLZ"
                     /note="basic region leucin zipper; smart00338"
                     /db_xref="CDD:197664"
     Region          239..>360
                     /region_name="Vert_HS_TF"
                     /note="Vertebrate heat shock transcription factor;
                     pfam06546"
                     /db_xref="CDD:461944"
     Region          <432..>520
                     /region_name="Vert_HS_TF"
                     /note="Vertebrate heat shock transcription factor;
                     pfam06546"
                     /db_xref="CDD:461944"
     CDS             1..552
                     /gene="LOC144114356"
                     /coded_by="XM_077648026.1:112..1770"
                     /db_xref="GeneID:144114356"
ORIGIN      
        1 mhttevgvsn vpaflvklwk lvedekcndl iswssngqsf iihnqtqfak ellplyfkhn
       61 nmasfirqln mygfrkvtni dqglrsdree ieffhnffvr gqecllefik rkvpsgragg
      121 vgpddgrarn evlkellsnv gsmqgrqehm dqlladmkke nealwrevar lrqkhmkqqq
      181 ivekliqflv tmvqanrnit vkrkmplmlh dspnssskvp rltkgtfvtd yqvtspagsq
      241 msegpvirdv tdlmddfget sssvvsepma agqsprvvev saagtpvrsl aspaassdld
      301 agpivaspls tadvmeplcl ldplegdqss dalpcdilev vvdssdsspk epvaacskdl
      361 vtpavkaaqm tpatssvmqi tpaksvtpas pliseekpam lgdkqaangt sdlldscill
      421 edlplfagdt tppvaaspsg lpatstdvat eetsghnkva ttppanmemq valqdktagt
      481 trvfadhves idsdldwlhd qlmsgglsld tstlmglfsp edpltsrlgv stdpgsqlct
      541 fpflknqrsr wf
//
LOCUS       XP_077504153             391 aa            linear   INV 25-NOV-2025
DEFINITION  transforming growth factor beta regulator 1 [Amblyomma americanum].
ACCESSION   XP_077504153
VERSION     XP_077504153.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648027.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..391
                     /product="transforming growth factor beta regulator 1"
                     /calculated_mol_wt=42985
     Region          199..249
                     /region_name="FYRN"
                     /note="F/Y-rich N-terminus; pfam05964"
                     /db_xref="CDD:461787"
     Region          255..329
                     /region_name="FYRC"
                     /note="F/Y rich C-terminus; pfam05965"
                     /db_xref="CDD:461788"
     CDS             1..391
                     /gene="LOC144114357"
                     /coded_by="XM_077648027.1:335..1510"
                     /db_xref="GeneID:144114357"
ORIGIN      
        1 mnddvsddss dldagitaht ikyfprsvrh kgpsnlalkr anlmftmene ryrtkcrkle
       61 rfmkdlvies aalcdqvarv qesifvakee rrfllkkflh yrqqrdgpie tvrpavvsap
      121 aqvgsstppa tpaiggiseg adkpqkaskk vlkrrntkeg ladeaprpkr rkptgadkrl
      181 vapipldscg rplfpivlpg ckvhslgeiv tdragfhcqe slypvgycss riyasvlnan
      241 haciytctil dagtgprfei vaedaperpf vgsspdecyv allravnrtc gaevivpaar
      301 gadffalphp tiqnlvqscp garkcpnyrw vrfevsrsat deterldsdp ylsyaafrql
      361 clqprlaatv assepslppg vhikqepldt v
//
LOCUS       XP_077504154             408 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114358 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504154
VERSION     XP_077504154.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648028.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..408
                     /product="uncharacterized protein LOC144114358 isoform X1"
                     /calculated_mol_wt=44175
     Region          176..196
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(176,179,192,196)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          204..224
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(204,207,220,224)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(209,211,213,215..216,219..220,223,237,239,243..244,
                     247..248,251,265,267,269,271..272,275..276,279)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          217..241
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          232..252
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          256..>308
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          260..280
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          288..308
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          316..332
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..408
                     /gene="LOC144114358"
                     /coded_by="XM_077648028.1:138..1364"
                     /db_xref="GeneID:144114358"
ORIGIN      
        1 mastpeqvvc tptfvfpaaf petygkllia easscygfdp rqhlanfhat apaaatassv
       61 pqpvvqstee ashqglkavk tqprsslttl kipqvpasdf grslgsapva fpgiephkaa
      121 aapaqkveap vasespkgdk lwpcpsckvp fkaaselqqh lgqhtrsera vgavpcevcg
      181 klfasaervr ahvraahgek acacdicgsg fsyrcklldh mrthtgdkpf rcevcgksfs
      241 qknhltrham ihtgerpfpc dfcgrgfyrk dklarhrrvh tgerphvclv cgksygrrek
      301 larhlrahag erpfgcsqcg rrfleprdls rhkcaappcs sgqqqqqqqq qqqqqqpaes
      361 llllagggpl aglyqrlqqq gpstttanap pagqpqpdnp frprqlqa
//
LOCUS       XP_077504155             393 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114358 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504155
VERSION     XP_077504155.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648029.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..393
                     /product="uncharacterized protein LOC144114358 isoform X2"
                     /calculated_mol_wt=42546
     Region          161..181
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(161,164,177,181)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          189..209
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(189,192,205,209)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(194,196,198,200..201,204..205,208,222,224,228..229,
                     232..233,236,250,252,254,256..257,260..261,264)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          202..226
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          217..237
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          241..>293
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          245..265
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          273..293
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          301..317
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..393
                     /gene="LOC144114358"
                     /coded_by="XM_077648029.1:138..1319"
                     /db_xref="GeneID:144114358"
ORIGIN      
        1 mastpeqvvc tptfvfpaaf pgfdprqhla nfhatapaaa tassvpqpvv qsteeashqg
       61 lkavktqprs slttlkipqv pasdfgrslg sapvafpgie phkaaaapaq kveapvases
      121 pkgdklwpcp sckvpfkaas elqqhlgqht rseravgavp cevcgklfas aervrahvra
      181 ahgekacacd icgsgfsyrc klldhmrtht gdkpfrcevc gksfsqknhl trhamihtge
      241 rpfpcdfcgr gfyrkdklar hrrvhtgerp hvclvcgksy grreklarhl rahagerpfg
      301 csqcgrrfle prdlsrhkca appcssgqqq qqqqqqqqqq qpaeslllla gggplaglyq
      361 rlqqqgpstt tanappagqp qpdnpfrprq lqa
//
LOCUS       XP_077504156             362 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114358 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504156
VERSION     XP_077504156.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648030.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..362
                     /product="uncharacterized protein LOC144114358 isoform X3"
                     /calculated_mol_wt=38109
     Region          144..164
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Region          176..196
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(176,179,192,196)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          204..224
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(204,207,220,224)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(209,211,213,215..216,219..220,223,237,239,243..244,
                     247..248,251,265,267,269,271..272,275..276,279)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          217..241
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          227..>281
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          232..252
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          260..280
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..362
                     /gene="LOC144114358"
                     /coded_by="XM_077648030.1:137..1225"
                     /db_xref="GeneID:144114358"
ORIGIN      
        1 mastpeqvvc tptfvfpaaf petygkllia easscygfdp rqhlanfhat apaaatassv
       61 pqpvvqstee ashqglkavk tqprsslttl kipqvpasdf grslgsapva fpgiephkaa
      121 aapaqkveap vasespkgdk lwpcpsckvp fkaaselqqh lgqhtrsera vgavpcevcg
      181 klfasaervr ahvraahgek acacdicgsg fsyrcklldh mrthtgdkpf rcevcgksfs
      241 qknhltrham ihtgerpfpc dfcgrgfyrk dklarhrrvh tvgsssssss sssssssqpr
      301 ascsspgvap srgstsgsss kvpappppmp pplgspnqtt pfgpgsfkpd wqppaslasp
      361 rh
//
LOCUS       XP_077504157             278 aa            linear   INV 25-NOV-2025
DEFINITION  proteasome beta5 subunit [Amblyomma americanum].
ACCESSION   XP_077504157
VERSION     XP_077504157.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648031.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..278
                     /product="proteasome beta5 subunit"
                     /calculated_mol_wt=30643
     Region          44..275
                     /region_name="Ntn_hydrolase"
                     /note="The Ntn hydrolases (N-terminal nucleophile) are a
                     diverse superfamily of of enzymes that are activated
                     autocatalytically via an N-terminally lcated nucleophilic
                     amino acid. N-terminal nucleophile (NTN-) hydrolase
                     superfamily, which contains a...; cl00467"
                     /db_xref="CDD:469781"
     Site            order(74,90,92,106,203,240,243..244)
                     /site_type="active"
                     /db_xref="CDD:239730"
     Site            order(92,95,97..102,122,124,139,151,154..155,157..158,161,
                     164,188,190,192..195,198,206..207,209..210,213..215,231,
                     238..241,244..245)
                     /site_type="other"
                     /note="beta subunit interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:239730"
     CDS             1..278
                     /gene="Prosbeta5"
                     /coded_by="XM_077648031.1:63..899"
                     /db_xref="GeneID:144114359"
ORIGIN      
        1 maleevvkfn lrsgfgkasl dqqdllaygd afgsnfkfht qlalppfpnp qegmaallkd
       61 dsgkqikmhy dkgtttlafk ynggvivaad sratggeyig sqtvkkiivi nkyllgtmag
      121 gaadcvywqr vlaercriye lrnrerisva aaskllanil ynykgmglsl gvmicgwdkr
      181 gpglyyvdne gnrlsgnlys cgsgstyayg ildngysydl sdeeaielgr kaifhatfrd
      241 sasggivrvy rvketgweci seedckdlyy nyqaenmq
//
LOCUS       XP_077504158             672 aa            linear   INV 25-NOV-2025
DEFINITION  phosphoenolpyruvate carboxykinase, cytosolic [GTP]-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077504158
VERSION     XP_077504158.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648032.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..672
                     /product="phosphoenolpyruvate carboxykinase, cytosolic
                     [GTP]-like isoform X1"
                     /calculated_mol_wt=73831
     Region          74..665
                     /region_name="PEPCK_GTP"
                     /note="Phosphoenolpyruvate carboxykinase (PEPCK), a
                     critical gluconeogenic enzyme, catalyzes the first
                     committed step in the diversion of tricarboxylic acid
                     cycle intermediates toward gluconeogenesis. It catalyzes
                     the reversible decarboxylation and...; cd00819"
                     /db_xref="CDD:238417"
     Site            order(134,283,285,291..292,312,334..340,358..359,381,383,
                     392,453,575,578,581)
                     /site_type="active"
                     /db_xref="CDD:238417"
     Site            order(134,283,285,291..292,381,453)
                     /site_type="other"
                     /note="substrate-binding site [chemical binding]"
                     /db_xref="CDD:238417"
     Site            order(292,312,339,358..359,381)
                     /site_type="metal-binding"
                     /note="metal-binding site [ion binding]"
                     /db_xref="CDD:238417"
     Site            order(335,337,484,564..565,575,578,581)
                     /site_type="other"
                     /note="GTP binding site [chemical binding]"
                     /db_xref="CDD:238417"
     CDS             1..672
                     /gene="LOC144114360"
                     /coded_by="XM_077648032.1:462..2480"
                     /db_xref="GeneID:144114360"
ORIGIN      
        1 msasgstqll vrqgnptapp pqrvgpgpda grslkclqsq laqpmgvcar alssspalhq
       61 qpqpatlsal phdvrayvee kqrlcqpsav hicdgseaen shlldlmlka gsivplpkyr
      121 ncylartdpa dvarvesktf isterredti ptpkegvkgt lgnwmspgdl qkalnerfpg
      181 cmkgrtmyvi pfsmgplgsp lskigiqltd spyvvasmri mtrmgsevlr tlgngpfikc
      241 lhsvgqplpm ktppvnnwpc npektiishi pdnneicsfg sgyggnsllg kkcfalrlgs
      301 ilaqregwla ehmlilgvtn pkgekkyiaa afpsacgktn lammtptlpg ykaecvgddi
      361 awmkfddqgv lrainpeygf fgvapgtsma tnpnamktve entmftnvae tsdggfwweg
      421 mpepspgiri kswrgeenwt ralgtpaahp nsrfctpasq cpiidpawed pkgvpisail
      481 fggrrpqgvp lvyeafswqh gvfigsamrs eataaaehkg kvimhdpfam rpffgynftn
      541 yqrhwlslgs kperklpkif hvnwfrkgsd gkflwpgfge nsrvidwilr rvdeesgtak
      601 espigylpad galnldglqe pvnmkelfgl skdfwlqecr evrqyfddql gkelpseiaq
      661 eldqlekrvk am
//
LOCUS       XP_077504159             649 aa            linear   INV 25-NOV-2025
DEFINITION  phosphoenolpyruvate carboxykinase, cytosolic [GTP]-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077504159
VERSION     XP_077504159.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648033.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..649
                     /product="phosphoenolpyruvate carboxykinase, cytosolic
                     [GTP]-like isoform X2"
                     /calculated_mol_wt=71705
     Region          51..642
                     /region_name="PEPCK_GTP"
                     /note="Phosphoenolpyruvate carboxykinase (PEPCK), a
                     critical gluconeogenic enzyme, catalyzes the first
                     committed step in the diversion of tricarboxylic acid
                     cycle intermediates toward gluconeogenesis. It catalyzes
                     the reversible decarboxylation and...; cd00819"
                     /db_xref="CDD:238417"
     Site            order(111,260,262,268..269,289,311..317,335..336,358,360,
                     369,430,552,555,558)
                     /site_type="active"
                     /db_xref="CDD:238417"
     Site            order(111,260,262,268..269,358,430)
                     /site_type="other"
                     /note="substrate-binding site [chemical binding]"
                     /db_xref="CDD:238417"
     Site            order(269,289,316,335..336,358)
                     /site_type="metal-binding"
                     /note="metal-binding site [ion binding]"
                     /db_xref="CDD:238417"
     Site            order(312,314,461,541..542,552,555,558)
                     /site_type="other"
                     /note="GTP binding site [chemical binding]"
                     /db_xref="CDD:238417"
     CDS             1..649
                     /gene="LOC144114360"
                     /coded_by="XM_077648033.1:185..2134"
                     /db_xref="GeneID:144114360"
ORIGIN      
        1 mkiitclprs lkclqsqlaq pmgvcarals sspalhqqpq patlsalphd vrayveekqr
       61 lcqpsavhic dgseaenshl ldlmlkagsi vplpkyrncy lartdpadva rvesktfist
      121 erredtiptp kegvkgtlgn wmspgdlqka lnerfpgcmk grtmyvipfs mgplgsplsk
      181 igiqltdspy vvasmrimtr mgsevlrtlg ngpfikclhs vgqplpmktp pvnnwpcnpe
      241 ktiishipdn neicsfgsgy ggnsllgkkc falrlgsila qregwlaehm lilgvtnpkg
      301 ekkyiaaafp sacgktnlam mtptlpgyka ecvgddiawm kfddqgvlra inpeygffgv
      361 apgtsmatnp namktveent mftnvaetsd ggfwwegmpe pspgiriksw rgeenwtral
      421 gtpaahpnsr fctpasqcpi idpawedpkg vpisailfgg rrpqgvplvy eafswqhgvf
      481 igsamrseat aaaehkgkvi mhdpfamrpf fgynftnyqr hwlslgskpe rklpkifhvn
      541 wfrkgsdgkf lwpgfgensr vidwilrrvd eesgtakesp igylpadgal nldglqepvn
      601 mkelfglskd fwlqecrevr qyfddqlgke lpseiaqeld qlekrvkam
//
LOCUS       XP_077504160             717 aa            linear   INV 25-NOV-2025
DEFINITION  vesicular acetylcholine transporter isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504160
VERSION     XP_077504160.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648034.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..717
                     /product="vesicular acetylcholine transporter isoform X1"
                     /calculated_mol_wt=79496
     Region          70..681
                     /region_name="Carn_acyltransf"
                     /note="Choline/Carnitine o-acyltransferase; pfam00755"
                     /db_xref="CDD:459926"
     CDS             1..717
                     /gene="VAChT"
                     /coded_by="XM_077648034.1:79..2232"
                     /db_xref="GeneID:144114361"
ORIGIN      
        1 mhkaprasgt fkalarnras rprtpleras esgrrfqtga fslssqggsr swetmatsqk
       61 acgelerafv palaatlqry ldslvavvss paqmentrrl vhqfmhgqgn ntasqdgkgg
      121 rdplgpllqe klklfassrd nwvtdlwldd mylnnsqplp vnsspfylfp kqcfatsadq
      181 arfaarfief avlfkrkids gtltpdvgrg agqlqcmqty khffpayrrp gptkddllld
      241 tasqhqdhvi vvcrdqffkl rlpldqggpl dvealtrqll sikqqakdps erqppvgaft
      301 teqrrtwagi yaklarsnvn qsslqcletc llvvcldrpl sslrlrhyas lrresvtldl
      361 gaqaahllhg gsvadgnaan rwydkfmqiv vsrdgingli cehsasegvt virfceeflq
      421 hmqqqyqqqp qapppgspsi rassvcaqyp vsrlswdlgd dvlkalqdst kaintlaedv
      481 dlyilhfpny gknfikeqri spdvyiqlal qltyykvhrk lvstyesasl rkfhlgrvdn
      541 iraataeala fvqamcdavp ateedkiklf leavakgtei ltytvngdgp dnhllglrem
      601 arlhempaai fsdksyadfl hfrlstsqls adkgilggyg avvpdgygca ynvgadqvsf
      661 cvssffsspe tssdffalsl egsllqmrel clkratmlnm appparkipg prdryfa
//
LOCUS       XP_077504161             703 aa            linear   INV 25-NOV-2025
DEFINITION  vesicular acetylcholine transporter isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504161
VERSION     XP_077504161.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648035.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..703
                     /product="vesicular acetylcholine transporter isoform X2"
                     /calculated_mol_wt=78692
     Region          70..628
                     /region_name="Carn_acyltransf"
                     /note="Choline/Carnitine o-acyltransferase; pfam00755"
                     /db_xref="CDD:459926"
     CDS             1..703
                     /gene="VAChT"
                     /coded_by="XM_077648035.1:79..2190"
                     /db_xref="GeneID:144114361"
ORIGIN      
        1 mhkaprasgt fkalarnras rprtpleras esgrrfqtga fslssqggsr swetmatsqk
       61 acgelerafv palaatlqry ldslvavvss paqmentrrl vhqfmhgqgn ntasqdgkgg
      121 rdplgpllqe klklfassrd nwvtdlwldd mylnnsqplp vnsspfylfp kqcfatsadq
      181 arfaarfief avlfkrkids gtltpdvgrg agqlqcmqty khffpayrrp gptkddllld
      241 tasqhqdhvi vvcrdqffkl rlpldqggpl dvealtrqll sikqqakdps erqppvgaft
      301 teqrrtwagi yaklarsnvn qsslqcletc llvvcldrpl sslrlrhyas lrresvtldl
      361 gaqaahllhg gsvadgnaan rwydkfmqiv vsrdgingli cehsasegvt virfceeflq
      421 hmqqqyqqqp qapppgspsi rassvcaqyp vsrlswdlgd dvlkalqdst kaintlaedv
      481 dlyilhfpny gknfikeqri spdvyiqlal qltyykvhrk lvstyesasl rkfhlgrvdn
      541 iraataeala fvqamcdavp ateedkiklf leavakgtei ltytvngdgp dnhllglrem
      601 arlhempaai fsdksyadfl hfrlstsqks largiaishe eiqltlthkv kkrvlrrail
      661 ffstkiklwd ffvfmpfsgc llfcvllvfv lecilhslcg htf
//
LOCUS       XP_077504162             663 aa            linear   INV 25-NOV-2025
DEFINITION  vesicular acetylcholine transporter isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504162
VERSION     XP_077504162.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648036.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..663
                     /product="vesicular acetylcholine transporter isoform X3"
                     /calculated_mol_wt=73578
     Region          16..627
                     /region_name="Carn_acyltransf"
                     /note="Choline/Carnitine o-acyltransferase; pfam00755"
                     /db_xref="CDD:459926"
     CDS             1..663
                     /gene="VAChT"
                     /coded_by="XM_077648036.1:86..2077"
                     /db_xref="GeneID:144114361"
ORIGIN      
        1 matsqkacge lerafvpala atlqryldsl vavvsspaqm entrrlvhqf mhgqgnntas
       61 qdgkggrdpl gpllqeklkl fassrdnwvt dlwlddmyln nsqplpvnss pfylfpkqcf
      121 atsadqarfa arfiefavlf krkidsgtlt pdvgrgagql qcmqtykhff payrrpgptk
      181 ddllldtasq hqdhvivvcr dqffklrlpl dqggpldvea ltrqllsikq qakdpserqp
      241 pvgaftteqr rtwagiyakl arsnvnqssl qcletcllvv cldrplsslr lrhyaslrre
      301 svtldlgaqa ahllhggsva dgnaanrwyd kfmqivvsrd ginglicehs asegvtvirf
      361 ceeflqhmqq qyqqqpqapp pgspsirass vcaqypvsrl swdlgddvlk alqdstkain
      421 tlaedvdlyi lhfpnygknf ikeqrispdv yiqlalqlty ykvhrklvst yesaslrkfh
      481 lgrvdniraa taealafvqa mcdavpatee dkiklfleav akgteiltyt vngdgpdnhl
      541 lglremarlh empaaifsdk syadflhfrl stsqlsadkg ilggygavvp dgygcaynvg
      601 adqvsfcvss ffsspetssd ffalslegsl lqmrelclkr atmlnmappp arkipgprdr
      661 yfa
//
LOCUS       XP_077504163             635 aa            linear   INV 25-NOV-2025
DEFINITION  vesicular acetylcholine transporter isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077504163
VERSION     XP_077504163.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648037.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..635
                     /product="vesicular acetylcholine transporter isoform X4"
                     /calculated_mol_wt=70548
     Region          61..599
                     /region_name="Carn_acyltransf"
                     /note="Choline/Carnitine o-acyltransferase; pfam00755"
                     /db_xref="CDD:459926"
     CDS             1..635
                     /gene="VAChT"
                     /coded_by="XM_077648037.1:79..1986"
                     /db_xref="GeneID:144114361"
ORIGIN      
        1 mhkaprasgt fkalarnras rprtpleras esgrrfqtga fslssqggsr swetmatsqk
       61 vtdlwlddmy lnnsqplpvn sspfylfpkq cfatsadqar faarfiefav lfkrkidsgt
      121 ltpdvgrgag qlqcmqtykh ffpayrrpgp tkddllldta sqhqdhvivv crdqffklrl
      181 pldqggpldv ealtrqllsi kqqakdpser qppvgaftte qrrtwagiya klarsnvnqs
      241 slqcletcll vvcldrplss lrlrhyaslr resvtldlga qaahllhggs vadgnaanrw
      301 ydkfmqivvs rdginglice hsasegvtvi rfceeflqhm qqqyqqqpqa pppgspsira
      361 ssvcaqypvs rlswdlgddv lkalqdstka intlaedvdl yilhfpnygk nfikeqrisp
      421 dvyiqlalql tyykvhrklv styesaslrk fhlgrvdnir aataealafv qamcdavpat
      481 eedkiklfle avakgteilt ytvngdgpdn hllglremar lhempaaifs dksyadflhf
      541 rlstsqlsad kgilggygav vpdgygcayn vgadqvsfcv ssffsspets sdffalsleg
      601 sllqmrelcl kratmlnmap pparkipgpr dryfa
//
LOCUS       XP_077504164             581 aa            linear   INV 25-NOV-2025
DEFINITION  vesicular acetylcholine transporter isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077504164
VERSION     XP_077504164.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648038.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..581
                     /product="vesicular acetylcholine transporter isoform X5"
                     /calculated_mol_wt=64630
     Region          7..545
                     /region_name="Carn_acyltransf"
                     /note="Choline/Carnitine o-acyltransferase; pfam00755"
                     /db_xref="CDD:459926"
     CDS             1..581
                     /gene="VAChT"
                     /coded_by="XM_077648038.1:83..1828"
                     /db_xref="GeneID:144114361"
ORIGIN      
        1 matsqkvtdl wlddmylnns qplpvnsspf ylfpkqcfat sadqarfaar fiefavlfkr
       61 kidsgtltpd vgrgagqlqc mqtykhffpa yrrpgptkdd llldtasqhq dhvivvcrdq
      121 ffklrlpldq ggpldvealt rqllsikqqa kdpserqppv gaftteqrrt wagiyaklar
      181 snvnqsslqc letcllvvcl drplsslrlr hyaslrresv tldlgaqaah llhggsvadg
      241 naanrwydkf mqivvsrdgi nglicehsas egvtvirfce eflqhmqqqy qqqpqapppg
      301 spsirassvc aqypvsrlsw dlgddvlkal qdstkaintl aedvdlyilh fpnygknfik
      361 eqrispdvyi qlalqltyyk vhrklvstye saslrkfhlg rvdniraata ealafvqamc
      421 davpateedk iklfleavak gteiltytvn gdgpdnhllg lremarlhem paaifsdksy
      481 adflhfrlst sqlsadkgil ggygavvpdg ygcaynvgad qvsfcvssff sspetssdff
      541 alslegsllq mrelclkrat mlnmapppar kipgprdryf a
//
LOCUS       XP_077504165             577 aa            linear   INV 25-NOV-2025
DEFINITION  vesicular acetylcholine transporter isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077504165
VERSION     XP_077504165.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648039.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..577
                     /product="vesicular acetylcholine transporter isoform X6"
                     /calculated_mol_wt=63848
     Region          33..455
                     /region_name="MFS_SLC18A3_VAChT"
                     /note="Vesicular acetylcholine transporter (VAChT) and
                     similar transporters of the Major Facilitator Superfamily;
                     cd17383"
                     /db_xref="CDD:340941"
     Site            order(43..44,47..48,51,123,173..174,176..178,181,202,
                     205..206,209,283,286..287,290..292,295,323,327,376..377,
                     381,385,406,409..410,413..414,417)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340941"
     CDS             1..577
                     /gene="VAChT"
                     /coded_by="XM_077648039.1:237..1970"
                     /db_xref="GeneID:144114361"
ORIGIN      
        1 mavlpfinqd tsqlwrrlnd kieepksqrr liliivcval lldnmlymvi vpiipqylrs
       61 idawythteg gnmssvvvna tvngvvtnrt ewrrtgghiv yegeesavgv lfaskaivql
      121 fvnpfsgali drigydipml fglsimflst aifacgtsyg vlffarslqg vgsafadtsg
      181 lamiadrfte etertkalgi alafisfgcl vappfgglly efagkevpfi ilslvslidg
      241 flllfvmqpv kqqmramgve rpkgtpiwrl fidpyiavaa galmmsnvsl afleptisiw
      301 mkdnmhvddw qigliwlpaf fphvagvylt vrmarqypqy qwaiacfgla leglssfivp
      361 farsywvliv plsgicfgia qvdtsllptl gylvdvryvs vygsiyaiad isyslayaig
      421 piiaggives igftalnifi aisnllycpl lmslrhiydy kpfeseanil mqdppakeyq
      481 tyrlqdgaaa plpvtnhvqq tsfmeggtva taqgqtqqqq lqqlpvtgkp ptpqhqqqqr
      541 pphkrpaakk hvrvrgyrss dmemmitsse eeegdle
//
LOCUS       XP_077504166             513 aa            linear   INV 25-NOV-2025
DEFINITION  proteoglycan Cow [Amblyomma americanum].
ACCESSION   XP_077504166
VERSION     XP_077504166.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648040.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..513
                     /product="proteoglycan Cow"
                     /calculated_mol_wt=58162
     Region          171..215
                     /region_name="Kazal_2"
                     /note="Kazal-type serine protease inhibitor domain;
                     pfam07648"
                     /db_xref="CDD:400135"
     Region          303..413
                     /region_name="EFh_SPARC_EC"
                     /note="EF-hand, extracellular calcium-binding (EC) motif,
                     found in secreted protein acidic and rich in cysteine
                     (SPARC)-like proteins; cl25349"
                     /db_xref="CDD:330171"
     Region          349..380
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320009"
     Site            order(361,365,372,393,395,397,404)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320009"
     Region          381..411
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320009"
     Region          415..474
                     /region_name="TY"
                     /note="Thyroglobulin type I repeats.; The N-terminal
                     region of human thyroglobulin contains 11 type-1 repeats
                     TY repeats are proposed to be inhibitors of cysteine
                     proteases; cd00191"
                     /db_xref="CDD:238114"
     Site            order(420,430,448,452)
                     /site_type="active"
                     /note="protease interaction site [active]"
                     /db_xref="CDD:238114"
     CDS             1..513
                     /gene="Cow"
                     /coded_by="XM_077648040.1:519..2060"
                     /db_xref="GeneID:144114362"
ORIGIN      
        1 mhtdkkawlw vvvlafllct aysatikdks kdseewdfef eehsrdesst qkdsmkdlka
       61 tynawsfada dlcrtvqckp tqecliqden taicvsakkv kkmkkaslsg swgsydrqqs
      121 tampltttaa gtdsaaavss takpwssaea eddddddqyl eddssqemer cppcpvfkad
      181 yvcgtdnqty ssecrlqyhn crqrtsvqvs ckgfcpckav ksleeqqres rqkarwdrym
      241 aklqntidke kkankdatgk paafnnrlks kfsskdswas kdghnsvmtn kvsspvvskd
      301 kgctnddlqa mgdrlldwfs vvmsdhqlst qvstrrrthr vsdyhlpeck pevgwmfhhl
      361 dsdgnllltl relydlehdd rerclqpyla vcdlnrdsfl stiewcacfn ksqrpcaaal
      421 rrvtpgliga yipqcdeegy flpaqchssa gmcwcvdrhg aefantrrrd rpdceslmnk
      481 tnrdksledd teedsdevkd nkdegsgdri qdf
//
LOCUS       XP_077504167             383 aa            linear   INV 25-NOV-2025
DEFINITION  alcohol dehydrogenase class-3 Fdh [Amblyomma americanum].
ACCESSION   XP_077504167
VERSION     XP_077504167.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648041.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..383
                     /product="alcohol dehydrogenase class-3 Fdh"
                     /calculated_mol_wt=41170
     Region          15..382
                     /region_name="alcohol_DH_class_III"
                     /note="class III alcohol dehydrogenases; cd08300"
                     /db_xref="CDD:176260"
     Site            order(54,56,65..67,76,102..103,183,303,327)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:176260"
     Site            order(54,76..77,183)
                     /site_type="other"
                     /note="catalytic Zn binding site [ion binding]"
                     /db_xref="CDD:176260"
     Site            order(55..56,183,187,208..212,232..233,237,277..278,
                     282..283,301..302,326..328,378)
                     /site_type="other"
                     /note="NAD binding site [chemical binding]"
                     /db_xref="CDD:176260"
     Site            order(106,109,112,120)
                     /site_type="other"
                     /note="structural Zn binding site [ion binding]"
                     /db_xref="CDD:176260"
     Site            order(110..111,114,116..117,119,121,268,273,284..285,
                     292..295,304,307..314,317..319,322..327)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:176260"
     CDS             1..383
                     /gene="Fdh"
                     /coded_by="XM_077648041.1:350..1501"
                     /db_xref="GeneID:144114363"
ORIGIN      
        1 mstggpsdat qgkpiqcraa vawepsrplq ietievavpk egevrikvlh tgvchtdayt
       61 lggldpegtf pcilgheggg ivesvgpnvk gfkpgdhvip lyipqckeck fckhpntnlc
      121 skirttqgrg lmpdgttrft cqgksiyhfm gtstfseytv vaeislckvd paapldkvcl
      181 lgcavstgyg aalnvaritk dstiaiwglg avglavamga kergaqtiig idineskfpk
      241 akefgctefi npskltvpit qyliektdgg ldftfecign ietmraales chkgwgesii
      301 lgvapagkei strpfmlvtg rvwrgcafgg wksvdqvpel vnrymkkvik ldefvsytmp
      361 lekindafkl lhdglairsv idl
//
LOCUS       XP_077504168             237 aa            linear   INV 25-NOV-2025
DEFINITION  SPRY domain-containing SOCS box protein SP555 [Amblyomma
            americanum].
ACCESSION   XP_077504168
VERSION     XP_077504168.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648042.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..237
                     /product="SPRY domain-containing SOCS box protein SP555"
                     /calculated_mol_wt=26992
     Region          26..209
                     /region_name="SPRY_SOCS3"
                     /note="SPRY domain in the suppressor of cytokine signaling
                     3 (SOCS3) family; cd12876"
                     /db_xref="CDD:293936"
     Region          200..234
                     /region_name="SOCS"
                     /note="SOCS (suppressors of cytokine signaling) box. The
                     SOCS box is found in the C-terminal region of CIS/SOCS
                     family proteins (in combination with a SH2 domain), ASBs
                     (ankyrin repeat-containing proteins with a SOCS box), SSBs
                     (SPRY domain-containing proteins...; cl02533"
                     /db_xref="CDD:470605"
     CDS             1..237
                     /gene="SP555"
                     /coded_by="XM_077648042.1:103..816"
                     /db_xref="GeneID:144114364"
ORIGIN      
        1 medadgqsgp dcrlepwnwg vedhswawst thkspevlll gprqaqfhpr wssgtagvri
       61 arplardrrs ywevhvshrv fgtsmmwglc taqaklhana fidllghdqn gwglshkgll
      121 whnnrwrsft rpfreneatv lgllfdgrrg tltyykdgic lgvafqdlhr vreplypavs
      181 staaktemsl anarrdyagl qdrcravilk qtagpdavre lrlplrlqry llheedi
//
LOCUS       XP_077504169             687 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114365 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504169
VERSION     XP_077504169.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648043.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..687
                     /product="uncharacterized protein LOC144114365 isoform X1"
                     /calculated_mol_wt=77179
     Region          2..>605
                     /region_name="FHY3"
                     /note="Protein FAR-RED ELONGATED HYPOCOTYL 3; Provisional;
                     PLN03097"
                     /db_xref="CDD:178645"
     Region          274..370
                     /region_name="MULE"
                     /note="MULE transposase domain; pfam10551"
                     /db_xref="CDD:463145"
     CDS             1..687
                     /gene="LOC144114365"
                     /coded_by="XM_077648043.1:122..2185"
                     /db_xref="GeneID:144114365"
ORIGIN      
        1 mdnnltdyvs vvqtmdsdht gyvsltppam eigakyssle elsaalrlye ethftqfwrs
       61 ssrtilgark kglkrpikde lvyseiifsc anggrkytsk stgarpnqrt fkigctarik
      121 vvasadgnfl evkllddvhn hpiseacfrq lpkqrrlaad dlleakklla fesdeeaiqn
      181 fivstgkvvl lrdlrnmgls gssqpehaam lqlmqpkeed nqrqgsgcal dalvlevqaa
      241 ggaldvlate dnslvgvfyq drfmqeafrs ypevvfvdav yktqgkgpll ilavedsnge
      301 tevvgfalca asddvtvrlv ierfvlhvgp davdrtltvm tdretvergi lrdvfpnate
      361 vicmfqvlra frrqmtaekm alsrdekasv vetlqamcha rddeayhelf qrlertcsas
      421 vlayfvahwh eirsqwvqgl qeaptlgnrt nnrlesvnqk vahivgkhet ldqlfhalkc
      481 fathlrierd qramqtilki aappnmtede rrymafltpy afkrvlhqmq aahsmeesde
      541 ayvsscsscn cwdstsymlp crhifferlr cglplyfeeg vaqrwtreyy ksachlfase
      601 skaqqqsstd asgeeeaees svaktspfek hritetgtlv qqlaacmlkl pagsyerava
      661 lvkdlktame egkevclvri estmevq
//
LOCUS       XP_077504170             579 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114365 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504170
VERSION     XP_077504170.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648044.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..579
                     /product="uncharacterized protein LOC144114365 isoform X2"
                     /calculated_mol_wt=65096
     Region          <142..>497
                     /region_name="FHY3"
                     /note="Protein FAR-RED ELONGATED HYPOCOTYL 3; Provisional;
                     PLN03097"
                     /db_xref="CDD:178645"
     Region          166..262
                     /region_name="MULE"
                     /note="MULE transposase domain; pfam10551"
                     /db_xref="CDD:463145"
     CDS             1..579
                     /gene="LOC144114365"
                     /coded_by="XM_077648044.1:364..2103"
                     /db_xref="GeneID:144114365"
ORIGIN      
        1 maagnthpsp qehdltkdgn flevkllddv hnhpiseacf rqlpkqrrla addlleakkl
       61 lafesdeeai qnfivstgkv vllrdlrnmg lsgssqpeha amlqlmqpke ednqrqgsgc
      121 aldalvlevq aaggaldvla tednslvgvf yqdrfmqeaf rsypevvfvd avyktqgkgp
      181 llilavedsn getevvgfal caasddvtvr lvierfvlhv gpdavdrtlt vmtdretver
      241 gilrdvfpna tevicmfqvl rafrrqmtae kmalsrdeka svvetlqamc harddeayhe
      301 lfqrlertcs asvlayfvah wheirsqwvq glqeaptlgn rtnnrlesvn qkvahivgkh
      361 etldqlfhal kcfathlrie rdqramqtil kiaappnmte derrymaflt pyafkrvlhq
      421 mqaahsmees deayvsscss cncwdstsym lpcrhiffer lrcglplyfe egvaqrwtre
      481 yyksachlfa seskaqqqss tdasgeeeae essvaktspf ekhritetgt lvqqlaacml
      541 klpagsyera valvkdlkta meegkevclv riestmevq
//
LOCUS       XP_077504171             579 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114365 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504171
VERSION     XP_077504171.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648045.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..579
                     /product="uncharacterized protein LOC144114365 isoform X2"
                     /calculated_mol_wt=65096
     Region          <142..>497
                     /region_name="FHY3"
                     /note="Protein FAR-RED ELONGATED HYPOCOTYL 3; Provisional;
                     PLN03097"
                     /db_xref="CDD:178645"
     Region          166..262
                     /region_name="MULE"
                     /note="MULE transposase domain; pfam10551"
                     /db_xref="CDD:463145"
     CDS             1..579
                     /gene="LOC144114365"
                     /coded_by="XM_077648045.1:212..1951"
                     /db_xref="GeneID:144114365"
ORIGIN      
        1 maagnthpsp qehdltkdgn flevkllddv hnhpiseacf rqlpkqrrla addlleakkl
       61 lafesdeeai qnfivstgkv vllrdlrnmg lsgssqpeha amlqlmqpke ednqrqgsgc
      121 aldalvlevq aaggaldvla tednslvgvf yqdrfmqeaf rsypevvfvd avyktqgkgp
      181 llilavedsn getevvgfal caasddvtvr lvierfvlhv gpdavdrtlt vmtdretver
      241 gilrdvfpna tevicmfqvl rafrrqmtae kmalsrdeka svvetlqamc harddeayhe
      301 lfqrlertcs asvlayfvah wheirsqwvq glqeaptlgn rtnnrlesvn qkvahivgkh
      361 etldqlfhal kcfathlrie rdqramqtil kiaappnmte derrymaflt pyafkrvlhq
      421 mqaahsmees deayvsscss cncwdstsym lpcrhiffer lrcglplyfe egvaqrwtre
      481 yyksachlfa seskaqqqss tdasgeeeae essvaktspf ekhritetgt lvqqlaacml
      541 klpagsyera valvkdlkta meegkevclv riestmevq
//
LOCUS       XP_077504172             296 aa            linear   INV 25-NOV-2025
DEFINITION  glyoxalase domain-containing protein 4-like [Amblyomma americanum].
ACCESSION   XP_077504172
VERSION     XP_077504172.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648046.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..296
                     /product="glyoxalase domain-containing protein 4-like"
                     /calculated_mol_wt=33152
     Region          3..128
                     /region_name="VOC"
                     /note="vicinal oxygen chelate (VOC) family; cl14632"
                     /db_xref="CDD:472697"
     Region          139..252
                     /region_name="GLOD4_C"
                     /note="C-terminal domain of human glyoxalase
                     domain-containing protein 4 and similar proteins; cd16357"
                     /db_xref="CDD:319964"
     Site            order(139,143,170,182,184,198,241,249,251)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:319964"
     CDS             1..296
                     /gene="LOC144114366"
                     /coded_by="XM_077648046.1:187..1077"
                     /db_xref="GeneID:144114366"
ORIGIN      
        1 mvrralhfvf kvgdrkttik ffkdvlgmrv lrheefedgc raecngpydl nwsktmigyg
       61 pevnhfvmel tynygigsya kgndfvavvi rsddvlknaq rhawpvekfe ghdaliapgg
      121 yrfvikprdd vdrrdpveev vlassdlart vsywqgllgm kmlersdrea afayddaqcr
      181 lrfrlsaapi drakaygrva fecpaaelpd ieqralaggh qvlkpltrld tpgkatvtvv
      241 iladpdghei cfvdaesfrl lsavdpeadr llreaidadk saewyrdiqg gdkpaa
//
LOCUS       XP_077504173             281 aa            linear   INV 25-NOV-2025
DEFINITION  serine/threonine-protein phosphatase 1-like [Amblyomma americanum].
ACCESSION   XP_077504173
VERSION     XP_077504173.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648047.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..281
                     /product="serine/threonine-protein phosphatase 1-like"
                     /calculated_mol_wt=31388
     Region          51..256
                     /region_name="MPP_PPP_family"
                     /note="phosphoprotein phosphatases of the
                     metallophosphatase superfamily, metallophosphatase domain;
                     cd00144"
                     /db_xref="CDD:277316"
     Site            order(54,56,83,87,111..112,166,184,226)
                     /site_type="active"
                     /db_xref="CDD:277316"
     Site            order(54,56,83,111,166,226)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:277316"
     CDS             1..281
                     /gene="LOC144114367"
                     /coded_by="XM_077648047.1:172..1017"
                     /db_xref="GeneID:144114367"
ORIGIN      
        1 mgallsvfls avrtyfhdaa spkhpfgfam ppeihktltp eyiesfkqvf vigdvhgcfe
       61 elmdllavak arekdtlklf vgdlvnkgpk sedvldylla nratcyavrg nhdevvlkey
      121 yasrshsyal taenvwinrl sarhahyleq lpytlllpsl ntiivhaglv pdqglstsiv
      181 nmvtmrnvii ddfwekggik atckdeerae pwgqvwngpq hvifghdakr klqkwqhctg
      241 ldtgcvygna ltgmfiagpr agelvsvkak cthqrprekk k
//
LOCUS       XP_077504174            1327 aa            linear   INV 25-NOV-2025
DEFINITION  ribonuclease 3 drosha isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504174
VERSION     XP_077504174.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648048.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1327
                     /product="ribonuclease 3 drosha isoform X1"
                     /calculated_mol_wt=151206
     Region          878..994
                     /region_name="RIBOc"
                     /note="Ribonuclease III family; smart00535"
                     /db_xref="CDD:197778"
     Site            order(885..886,889..890,893,896..897,900..901,903..905,
                     915,971,980)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:238333"
     Site            order(885,888,892,961,964)
                     /site_type="active"
                     /db_xref="CDD:238333"
     Site            order(888,961,964)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:238333"
     Region          1024..1248
                     /region_name="Rnc"
                     /note="dsRNA-specific ribonuclease [Transcription];
                     COG0571"
                     /db_xref="CDD:440336"
     CDS             1..1327
                     /gene="drosha"
                     /coded_by="XM_077648048.1:333..4316"
                     /db_xref="GeneID:144114368"
ORIGIN      
        1 mteatcivkt emhpsslnla nqgapsalvr aalascspqq qqqqhqqfga qaaalyraan
       61 pamatiphwa tpmttapfpv arpsipppip yramartgfd agmlhsqgql plqaqpqwnt
      121 sclpvlpiss lstgqyrsyh aaalqqltsq aytgtssqty tswvqegvsd vrnssveigr
      181 sshhsrdrsr errrshsrer rrrsspcsrs dvrqrsrdep rqrnsrrsqs hsppphsrrr
      241 dssrlssshs svtssshssr krerpsdkrg dcrekrrrkd eeeekekkgr skvrgspket
      301 graspeedeq sedemsanqe pepddaeeed yllesarwir sspaelyyqr dekvsglvtg
      361 taklralqdk frhdlleraa rvkskqepyn ppprksrlcn pkshpiastg tsdsssnesd
      421 sdddnameel evkkkhpyrl hqelwyndpg emndgplcrc spkaqrsgir hgiypgeepl
      481 sscgpnsnni grlhhyrivv tprtnftmkc pttikhdghd flfegfslls hwplpklptc
      541 rvirfnieya vlyleeqmpe nftveglelf tsylfsevle mldldwkahg dkdgcprfhf
      601 mprfarkled ngkellslnv vlqylltnnv plvsaadlpr lyktsqskwq ayadqvkgmi
      661 vvnpgmkpca lrvdqldrel cnaqgtvfpe ivhfgirppq lsyagnpmyq kawrdyvkfr
      721 hllankpkvl ytdrhkllqk evklqemrlk snlkrdvtvv vsskgffrtg imcdvvqhal
      781 llpvlvchlr fhksldfles iiqtefkdry llqlalthps yrenfgtnpd harnsltncg
      841 irqpeygdrr ihylntrkrg intlinimsr fgrqeetass ishnerlefl gdavveflss
      901 ihlffmfpdl eegglatyra aivqnqhlav lakkleleky mlyahgsdlc hdlelrhama
      961 ncfealmgal fldsgikvad rvfsttlfse pdlldvwvny pphplqdqvp egdrkwidsv
     1021 pllkklnefe mltgiefthi rllakafthr sigfnnltlg snqrleflgd tvlqlvasey
     1081 lykyfpehhe ghlsllrssl vnnrtqavvc ddlgmveyti ypypkaelkt kdradileaf
     1141 lgalfvdkgl eycnafcqvc ffprlqdfil nqdwndpksk lqqccltlrs mdggepdipv
     1201 ykvieckgpt ntrvytvavy frvkrlatgs ghsiqqaema aaskaleask elfpqlshqr
     1261 rvlerslkqr apskegersv lttkksrees tmdsrsrhss srphgsldrr rtggesrkrs
     1321 rskskea
//
LOCUS       XP_077504175            1326 aa            linear   INV 25-NOV-2025
DEFINITION  ribonuclease 3 drosha isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504175
VERSION     XP_077504175.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648049.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1326
                     /product="ribonuclease 3 drosha isoform X2"
                     /calculated_mol_wt=151135
     Region          877..993
                     /region_name="RIBOc"
                     /note="Ribonuclease III family; smart00535"
                     /db_xref="CDD:197778"
     Site            order(884..885,888..889,892,895..896,899..900,902..904,
                     914,970,979)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:238333"
     Site            order(884,887,891,960,963)
                     /site_type="active"
                     /db_xref="CDD:238333"
     Site            order(887,960,963)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:238333"
     Region          1023..1247
                     /region_name="Rnc"
                     /note="dsRNA-specific ribonuclease [Transcription];
                     COG0571"
                     /db_xref="CDD:440336"
     CDS             1..1326
                     /gene="drosha"
                     /coded_by="XM_077648049.1:333..4313"
                     /db_xref="GeneID:144114368"
ORIGIN      
        1 mteatcivkt emhpsslnla nqgapsalvr aalascspqq qqqqhqqfga qaaalyraan
       61 pamatiphwa tpmttapfpv arpsipppip yramartgfd agmlhsqgql plqaqpqwnt
      121 sclpvlpiss lssqyrsyha aalqqltsqa ytgtssqtyt swvqegvsdv rnssveigrs
      181 shhsrdrsre rrrshsrerr rrsspcsrsd vrqrsrdepr qrnsrrsqsh sppphsrrrd
      241 ssrlssshss vtssshssrk rerpsdkrgd crekrrrkde eeekekkgrs kvrgspketg
      301 raspeedeqs edemsanqep epddaeeedy llesarwirs spaelyyqrd ekvsglvtgt
      361 aklralqdkf rhdlleraar vkskqepynp pprksrlcnp kshpiastgt sdsssnesds
      421 dddnameele vkkkhpyrlh qelwyndpge mndgplcrcs pkaqrsgirh giypgeepls
      481 scgpnsnnig rlhhyrivvt prtnftmkcp ttikhdghdf lfegfsllsh wplpklptcr
      541 virfnieyav lyleeqmpen ftveglelft sylfsevlem ldldwkahgd kdgcprfhfm
      601 prfarkledn gkellslnvv lqylltnnvp lvsaadlprl yktsqskwqa yadqvkgmiv
      661 vnpgmkpcal rvdqldrelc naqgtvfpei vhfgirppql syagnpmyqk awrdyvkfrh
      721 llankpkvly tdrhkllqke vklqemrlks nlkrdvtvvv sskgffrtgi mcdvvqhall
      781 lpvlvchlrf hksldflesi iqtefkdryl lqlalthpsy renfgtnpdh arnsltncgi
      841 rqpeygdrri hylntrkrgi ntlinimsrf grqeetassi shnerleflg davveflssi
      901 hlffmfpdle egglatyraa ivqnqhlavl akklelekym lyahgsdlch dlelrhaman
      961 cfealmgalf ldsgikvadr vfsttlfsep dlldvwvnyp phplqdqvpe gdrkwidsvp
     1021 llkklnefem ltgiefthir llakafthrs igfnnltlgs nqrleflgdt vlqlvaseyl
     1081 ykyfpehheg hlsllrsslv nnrtqavvcd dlgmveytiy pypkaelktk dradileafl
     1141 galfvdkgle ycnafcqvcf fprlqdfiln qdwndpkskl qqccltlrsm dggepdipvy
     1201 kvieckgptn trvytvavyf rvkrlatgsg hsiqqaemaa askaleaske lfpqlshqrr
     1261 vlerslkqra pskegersvl ttkksreest mdsrsrhsss rphgsldrrr tggesrkrsr
     1321 skskea
//
LOCUS       XP_077504176            1272 aa            linear   INV 25-NOV-2025
DEFINITION  ribonuclease 3 drosha isoform X3 [Amblyomma americanum].
ACCESSION   XP_077504176
VERSION     XP_077504176.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648050.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1272
                     /product="ribonuclease 3 drosha isoform X3"
                     /calculated_mol_wt=144661
     Region          878..994
                     /region_name="RIBOc"
                     /note="Ribonuclease III family; smart00535"
                     /db_xref="CDD:197778"
     Site            order(885..886,889..890,893,896..897,900..901,903..905,
                     915,971,980)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:238333"
     Site            order(885,888,892,961,964)
                     /site_type="active"
                     /db_xref="CDD:238333"
     Site            order(888,961,964)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:238333"
     Region          1024..1248
                     /region_name="Rnc"
                     /note="dsRNA-specific ribonuclease [Transcription];
                     COG0571"
                     /db_xref="CDD:440336"
     CDS             1..1272
                     /gene="drosha"
                     /coded_by="XM_077648050.1:333..4151"
                     /db_xref="GeneID:144114368"
ORIGIN      
        1 mteatcivkt emhpsslnla nqgapsalvr aalascspqq qqqqhqqfga qaaalyraan
       61 pamatiphwa tpmttapfpv arpsipppip yramartgfd agmlhsqgql plqaqpqwnt
      121 sclpvlpiss lstgqyrsyh aaalqqltsq aytgtssqty tswvqegvsd vrnssveigr
      181 sshhsrdrsr errrshsrer rrrsspcsrs dvrqrsrdep rqrnsrrsqs hsppphsrrr
      241 dssrlssshs svtssshssr krerpsdkrg dcrekrrrkd eeeekekkgr skvrgspket
      301 graspeedeq sedemsanqe pepddaeeed yllesarwir sspaelyyqr dekvsglvtg
      361 taklralqdk frhdlleraa rvkskqepyn ppprksrlcn pkshpiastg tsdsssnesd
      421 sdddnameel evkkkhpyrl hqelwyndpg emndgplcrc spkaqrsgir hgiypgeepl
      481 sscgpnsnni grlhhyrivv tprtnftmkc pttikhdghd flfegfslls hwplpklptc
      541 rvirfnieya vlyleeqmpe nftveglelf tsylfsevle mldldwkahg dkdgcprfhf
      601 mprfarkled ngkellslnv vlqylltnnv plvsaadlpr lyktsqskwq ayadqvkgmi
      661 vvnpgmkpca lrvdqldrel cnaqgtvfpe ivhfgirppq lsyagnpmyq kawrdyvkfr
      721 hllankpkvl ytdrhkllqk evklqemrlk snlkrdvtvv vsskgffrtg imcdvvqhal
      781 llpvlvchlr fhksldfles iiqtefkdry llqlalthps yrenfgtnpd harnsltncg
      841 irqpeygdrr ihylntrkrg intlinimsr fgrqeetass ishnerlefl gdavveflss
      901 ihlffmfpdl eegglatyra aivqnqhlav lakkleleky mlyahgsdlc hdlelrhama
      961 ncfealmgal fldsgikvad rvfsttlfse pdlldvwvny pphplqdqvp egdrkwidsv
     1021 pllkklnefe mltgiefthi rllakafthr sigfnnltlg snqrleflgd tvlqlvasey
     1081 lykyfpehhe ghlsllrssl vnnrtqavvc ddlgmveyti ypypkaelkt kdradileaf
     1141 lgalfvdkgl eycnafcqvc ffprlqdfil nqdwndpksk lqqccltlrs mdggepdipv
     1201 ykvieckgpt ntrvytvavy frvkrlatgs ghsiqqaema aaskaleask apdrnvatgh
     1261 alnaprcmhr ak
//
LOCUS       XP_077504177            1253 aa            linear   INV 25-NOV-2025
DEFINITION  ribonuclease 3 drosha isoform X4 [Amblyomma americanum].
ACCESSION   XP_077504177
VERSION     XP_077504177.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648051.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1253
                     /product="ribonuclease 3 drosha isoform X4"
                     /calculated_mol_wt=142633
     Region          878..994
                     /region_name="RIBOc"
                     /note="Ribonuclease III family; smart00535"
                     /db_xref="CDD:197778"
     Site            order(885..886,889..890,893,896..897,900..901,903..905,
                     915,971,980)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:238333"
     Site            order(885,888,892,961,964)
                     /site_type="active"
                     /db_xref="CDD:238333"
     Site            order(888,961,964)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:238333"
     Region          1024..1248
                     /region_name="Rnc"
                     /note="dsRNA-specific ribonuclease [Transcription];
                     COG0571"
                     /db_xref="CDD:440336"
     CDS             1..1253
                     /gene="drosha"
                     /coded_by="XM_077648051.1:333..4094"
                     /db_xref="GeneID:144114368"
ORIGIN      
        1 mteatcivkt emhpsslnla nqgapsalvr aalascspqq qqqqhqqfga qaaalyraan
       61 pamatiphwa tpmttapfpv arpsipppip yramartgfd agmlhsqgql plqaqpqwnt
      121 sclpvlpiss lstgqyrsyh aaalqqltsq aytgtssqty tswvqegvsd vrnssveigr
      181 sshhsrdrsr errrshsrer rrrsspcsrs dvrqrsrdep rqrnsrrsqs hsppphsrrr
      241 dssrlssshs svtssshssr krerpsdkrg dcrekrrrkd eeeekekkgr skvrgspket
      301 graspeedeq sedemsanqe pepddaeeed yllesarwir sspaelyyqr dekvsglvtg
      361 taklralqdk frhdlleraa rvkskqepyn ppprksrlcn pkshpiastg tsdsssnesd
      421 sdddnameel evkkkhpyrl hqelwyndpg emndgplcrc spkaqrsgir hgiypgeepl
      481 sscgpnsnni grlhhyrivv tprtnftmkc pttikhdghd flfegfslls hwplpklptc
      541 rvirfnieya vlyleeqmpe nftveglelf tsylfsevle mldldwkahg dkdgcprfhf
      601 mprfarkled ngkellslnv vlqylltnnv plvsaadlpr lyktsqskwq ayadqvkgmi
      661 vvnpgmkpca lrvdqldrel cnaqgtvfpe ivhfgirppq lsyagnpmyq kawrdyvkfr
      721 hllankpkvl ytdrhkllqk evklqemrlk snlkrdvtvv vsskgffrtg imcdvvqhal
      781 llpvlvchlr fhksldfles iiqtefkdry llqlalthps yrenfgtnpd harnsltncg
      841 irqpeygdrr ihylntrkrg intlinimsr fgrqeetass ishnerlefl gdavveflss
      901 ihlffmfpdl eegglatyra aivqnqhlav lakkleleky mlyahgsdlc hdlelrhama
      961 ncfealmgal fldsgikvad rvfsttlfse pdlldvwvny pphplqdqvp egdrkwidsv
     1021 pllkklnefe mltgiefthi rllakafthr sigfnnltlg snqrleflgd tvlqlvasey
     1081 lykyfpehhe ghlsllrssl vnnrtqavvc ddlgmveyti ypypkaelkt kdradileaf
     1141 lgalfvdkgl eycnafcqvc ffprlqdfil nqdwndpksk lqqccltlrs mdggepdipv
     1201 ykvieckgpt ntrvytvavy frvkrlatgs ghsiqqaema aaskaleask gqr
//
LOCUS       XP_077504178            1013 aa            linear   INV 25-NOV-2025
DEFINITION  ribonuclease 3 drosha isoform X5 [Amblyomma americanum].
ACCESSION   XP_077504178
VERSION     XP_077504178.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648052.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1013
                     /product="ribonuclease 3 drosha isoform X5"
                     /calculated_mol_wt=116011
     Region          564..680
                     /region_name="RIBOc"
                     /note="Ribonuclease III family; smart00535"
                     /db_xref="CDD:197778"
     Site            order(571..572,575..576,579,582..583,586..587,589..591,
                     601,657,666)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:238333"
     Site            order(571,574,578,647,650)
                     /site_type="active"
                     /db_xref="CDD:238333"
     Site            order(574,647,650)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:238333"
     Region          710..934
                     /region_name="Rnc"
                     /note="dsRNA-specific ribonuclease [Transcription];
                     COG0571"
                     /db_xref="CDD:440336"
     CDS             1..1013
                     /gene="drosha"
                     /coded_by="XM_077648052.1:1199..4240"
                     /db_xref="GeneID:144114368"
ORIGIN      
        1 msanqepepd daeeedylle sarwirsspa elyyqrdekv sglvtgtakl ralqdkfrhd
       61 lleraarvks kqepynpppr ksrlcnpksh piastgtsds ssnesdsddd nameelevkk
      121 khpyrlhqel wyndpgemnd gplcrcspka qrsgirhgiy pgeeplsscg pnsnnigrlh
      181 hyrivvtprt nftmkcptti khdghdflfe gfsllshwpl pklptcrvir fnieyavlyl
      241 eeqmpenftv eglelftsyl fsevlemldl dwkahgdkdg cprfhfmprf arkledngke
      301 llslnvvlqy lltnnvplvs aadlprlykt sqskwqayad qvkgmivvnp gmkpcalrvd
      361 qldrelcnaq gtvfpeivhf girppqlsya gnpmyqkawr dyvkfrhlla nkpkvlytdr
      421 hkllqkevkl qemrlksnlk rdvtvvvssk gffrtgimcd vvqhalllpv lvchlrfhks
      481 ldflesiiqt efkdryllql althpsyren fgtnpdharn sltncgirqp eygdrrihyl
      541 ntrkrgintl inimsrfgrq eetassishn erleflgdav veflssihlf fmfpdleegg
      601 latyraaivq nqhlavlakk lelekymlya hgsdlchdle lrhamancfe almgalflds
      661 gikvadrvfs ttlfsepdll dvwvnypphp lqdqvpegdr kwidsvpllk klnefemltg
      721 iefthirlla kafthrsigf nnltlgsnqr leflgdtvlq lvaseylyky fpehheghls
      781 llrsslvnnr tqavvcddlg mveytiypyp kaelktkdra dileaflgal fvdkgleycn
      841 afcqvcffpr lqdfilnqdw ndpksklqqc cltlrsmdgg epdipvykvi eckgptntrv
      901 ytvavyfrvk rlatgsghsi qqaemaaask aleaskelfp qlshqrrvle rslkqrapsk
      961 egersvlttk ksreestmds rsrhsssrph gsldrrrtgg esrkrsrsks kea
//
LOCUS       XP_077504180            1261 aa            linear   INV 25-NOV-2025
DEFINITION  numb-associated kinase isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504180
VERSION     XP_077504180.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648054.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1261
                     /product="numb-associated kinase isoform X1"
                     /calculated_mol_wt=134761
     Region          30..302
                     /region_name="STKc_NAK_like"
                     /note="Catalytic domain of Numb-Associated Kinase
                     (NAK)-like Serine/Threonine kinases; cd14037"
                     /db_xref="CDD:270939"
     Site            order(40..44,48,60,62,92,113..116,119,121,162,164,
                     166..167,169,180,183,207..210)
                     /site_type="active"
                     /db_xref="CDD:270939"
     Site            order(40..44,48,60,62,92,113..116,119,162,164,166..167,
                     169,180)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270939"
     Site            order(44,119,121,162,164,166,183,207..210)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270939"
     Site            order(179..193,194..210)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270939"
     Region          <322..663
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..1261
                     /gene="Nak"
                     /coded_by="XM_077648054.1:593..4378"
                     /db_xref="GeneID:144114370"
ORIGIN      
        1 mkklfsrfer idqgaarean nfvgkvftvg rhtvtvedvi aeggfaivfl vkgsnnvkya
       61 lkrmfvneeh glnvckreiq iasnlsghkn iigfvdsvin hvgggvyevl mlmhyykghv
      121 lqlmndklhs gfseqevlki fcdiceavsr lhhcqtpivh rdlkvenili sdsghyvlcd
      181 fgsataksln pqtqgvavve eeilkyttla yrapemvely sgklittkad iwalgcllyk
      241 lcffslpfge salaiqsgnf tipdnsrysk qmhsliryml evqpekrpdi fqvsyvafsl
      301 agkecpvqnl hnspipnieq lpvpqcdseg rlssskpprs lqvpvaegts vaprqrpkgs
      361 qappgvgglp lipqnalpas rrptpttpqp qvppvtpnpg sdpkgetvtp dissppsevq
      421 eavpdyenmr spcaaaaapf gsntkpgllp pppqasasap ssaittpsqp sqpssklgss
      481 sleaffapyc ageqqnfanv avplgsngaa aqcavadqvp fstatngpva gvggsfvpsg
      541 salataavgl agqqqqgfqa psvsadgvga sqpasvpsse rlapvltpps sptpsqkshh
      601 rrnvsdtsaf vkpfvqetsq flapfevslg rassppesnp hwnpfaepas stavdptdpe
      661 dqlfgqefdk irrgsqssis nvksreslvm ssgdlvdpfg aapfnrsgkp grarnssgkt
      721 alhhlsavps qpggsldeea agyvplyedh gdgivirarh csgnssasqe gsqggrstke
      781 alasfgafar ipvedrskye kllddvdedy argessrksg ktageedkds igsasdlqar
      841 aelsedddar eisglrenkr lepskelfsv gkqpvatvds saravlpetg glqtrgfqdm
      901 fvghmegdkp llededqlse petkptaeee qliskvqvgl lplpkresfp tegalanrdg
      961 advftqapfr kprpprklse parppahavs tscvaqpaas smppftvlpa saaatpsttq
     1021 qhsytrpafs dyktaaqdyr adyqrlsvha nseseltrgh seadlfgsap fthlkttpnn
     1081 ktlfkegisv vpqpsgrhns vasmsvgsha paiptrtqsh qdlfgstpfa lesgslleat
     1141 vetalatvtr agnvaktlld spespnssde egslrkfrkd kkryqqlrte edammtedlv
     1201 eggrrvathl rasasrkdkr ktekasnafs nlsfedigse edaaassfva eahfrtsavs
     1261 k
//
LOCUS       XP_077504181            1255 aa            linear   INV 25-NOV-2025
DEFINITION  numb-associated kinase isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504181
VERSION     XP_077504181.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648055.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1255
                     /product="numb-associated kinase isoform X2"
                     /calculated_mol_wt=134137
     Region          30..302
                     /region_name="STKc_NAK_like"
                     /note="Catalytic domain of Numb-Associated Kinase
                     (NAK)-like Serine/Threonine kinases; cd14037"
                     /db_xref="CDD:270939"
     Site            order(40..44,48,60,62,92,113..116,119,121,162,164,
                     166..167,169,180,183,207..210)
                     /site_type="active"
                     /db_xref="CDD:270939"
     Site            order(40..44,48,60,62,92,113..116,119,162,164,166..167,
                     169,180)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270939"
     Site            order(44,119,121,162,164,166,183,207..210)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270939"
     Site            order(179..193,194..210)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270939"
     Region          <322..657
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..1255
                     /gene="Nak"
                     /coded_by="XM_077648055.1:593..4360"
                     /db_xref="GeneID:144114370"
ORIGIN      
        1 mkklfsrfer idqgaarean nfvgkvftvg rhtvtvedvi aeggfaivfl vkgsnnvkya
       61 lkrmfvneeh glnvckreiq iasnlsghkn iigfvdsvin hvgggvyevl mlmhyykghv
      121 lqlmndklhs gfseqevlki fcdiceavsr lhhcqtpivh rdlkvenili sdsghyvlcd
      181 fgsataksln pqtqgvavve eeilkyttla yrapemvely sgklittkad iwalgcllyk
      241 lcffslpfge salaiqsgnf tipdnsrysk qmhsliryml evqpekrpdi fqvsyvafsl
      301 agkecpvqnl hnspipnieq lpvpqcdseg rlssskpprs lqvpvaegts vaprqrpkgs
      361 qappgvgglp lipqnalpas rrptpttpqp qvppvtpnpg sdpkgetvtp dissppsedy
      421 enmrspcaaa aapfgsntkp gllppppqas asapssaitt psqpsqpssk lgsssleaff
      481 apycageqqn fanvavplgs ngaaaqcava dqvpfstatn gpvagvggsf vpsgsalata
      541 avglagqqqq gfqapsvsad gvgasqpasv psserlapvl tppssptpsq kshhrrnvsd
      601 tsafvkpfvq etsqflapfe vslgrasspp esnphwnpfa epasstavdp tdpedqlfgq
      661 efdkirrgsq ssisnvksre slvmssgdlv dpfgaapfnr sgkpgrarns sgktalhhls
      721 avpsqpggsl deeaagyvpl yedhgdgivi rarhcsgnss asqegsqggr stkealasfg
      781 afaripvedr skyekllddv dedyargess rksgktagee dkdsigsasd lqaraelsed
      841 ddareisglr enkrlepske lfsvgkqpva tvdssaravl petgglqtrg fqdmfvghme
      901 gdkplleded qlsepetkpt aeeeqliskv qvgllplpkr esfptegala nrdgadvftq
      961 apfrkprppr klseparppa havstscvaq paassmppft vlpasaaatp sttqqhsytr
     1021 pafsdyktaa qdyradyqrl svhansesel trghseadlf gsapfthlkt tpnnktlfke
     1081 gisvvpqpsg rhnsvasmsv gshapaiptr tqshqdlfgs tpfalesgsl leatvetala
     1141 tvtragnvak tlldspespn ssdeegslrk frkdkkryqq lrteedammt edlveggrrv
     1201 athlrasasr kdkrktekas nafsnlsfed igseedaaas sfvaeahfrt savsk
//
LOCUS       XP_077504182            1236 aa            linear   INV 25-NOV-2025
DEFINITION  numb-associated kinase isoform X3 [Amblyomma americanum].
ACCESSION   XP_077504182
VERSION     XP_077504182.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648056.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1236
                     /product="numb-associated kinase isoform X3"
                     /calculated_mol_wt=132255
     Region          30..302
                     /region_name="STKc_NAK_like"
                     /note="Catalytic domain of Numb-Associated Kinase
                     (NAK)-like Serine/Threonine kinases; cd14037"
                     /db_xref="CDD:270939"
     Site            order(40..44,48,60,62,92,113..116,119,121,162,164,
                     166..167,169,180,183,207..210)
                     /site_type="active"
                     /db_xref="CDD:270939"
     Site            order(40..44,48,60,62,92,113..116,119,162,164,166..167,
                     169,180)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270939"
     Site            order(44,119,121,162,164,166,183,207..210)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270939"
     Site            order(179..193,194..210)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270939"
     Region          <322..638
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..1236
                     /gene="Nak"
                     /coded_by="XM_077648056.1:593..4303"
                     /db_xref="GeneID:144114370"
ORIGIN      
        1 mkklfsrfer idqgaarean nfvgkvftvg rhtvtvedvi aeggfaivfl vkgsnnvkya
       61 lkrmfvneeh glnvckreiq iasnlsghkn iigfvdsvin hvgggvyevl mlmhyykghv
      121 lqlmndklhs gfseqevlki fcdiceavsr lhhcqtpivh rdlkvenili sdsghyvlcd
      181 fgsataksln pqtqgvavve eeilkyttla yrapemvely sgklittkad iwalgcllyk
      241 lcffslpfge salaiqsgnf tipdnsrysk qmhsliryml evqpekrpdi fqvsyvafsl
      301 agkecpvqnl hnspipnieq lpvpqcdseg rlssskpprs lqvpvaegts vaprqrpkgs
      361 qappgvgglp lipqnalpas rrptpttpqp qvppvtpnpd yenmrspcaa aaapfgsntk
      421 pgllppppqa sasapssait tpsqpsqpss klgsssleaf fapycageqq nfanvavplg
      481 sngaaaqcav adqvpfstat ngpvagvggs fvpsgsalat aavglagqqq qgfqapsvsa
      541 dgvgasqpas vpsserlapv ltppssptps qkshhrrnvs dtsafvkpfv qetsqflapf
      601 evslgrassp pesnphwnpf aepasstavd ptdpedqlfg qefdkirrgs qssisnvksr
      661 eslvmssgdl vdpfgaapfn rsgkpgrarn ssgktalhhl savpsqpggs ldeeaagyvp
      721 lyedhgdgiv irarhcsgns sasqegsqgg rstkealasf gafaripved rskyeklldd
      781 vdedyarges srksgktage edkdsigsas dlqaraelse dddareisgl renkrlepsk
      841 elfsvgkqpv atvdssarav lpetgglqtr gfqdmfvghm egdkpllede dqlsepetkp
      901 taeeeqlisk vqvgllplpk resfptegal anrdgadvft qapfrkprpp rklseparpp
      961 ahavstscva qpaassmppf tvlpasaaat psttqqhsyt rpafsdykta aqdyradyqr
     1021 lsvhansese ltrghseadl fgsapfthlk ttpnnktlfk egisvvpqps grhnsvasms
     1081 vgshapaipt rtqshqdlfg stpfalesgs lleatvetal atvtragnva ktlldspesp
     1141 nssdeegslr kfrkdkkryq qlrteedamm tedlveggrr vathlrasas rkdkrkteka
     1201 snafsnlsfe digseedaaa ssfvaeahfr tsavsk
//
LOCUS       XP_077504183            1219 aa            linear   INV 25-NOV-2025
DEFINITION  treslin-like [Amblyomma americanum].
ACCESSION   XP_077504183
VERSION     XP_077504183.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648057.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1219
                     /product="treslin-like"
                     /calculated_mol_wt=134499
     Region          <571..898
                     /region_name="Treslin_N"
                     /note="Treslin N-terminus; pfam15292"
                     /db_xref="CDD:464618"
     CDS             1..1219
                     /gene="LOC144114371"
                     /coded_by="XM_077648057.1:84..3743"
                     /db_xref="GeneID:144114371"
ORIGIN      
        1 mfnstkvvfl vdvasffsre drlsvtdyqq avnavkyccl kflthfgtkk trwgykfyss
       61 sglarqrmek rlfcdfnles fedfeddlvr rfehyrqacd aidrsgsrsv pekdppcsvl
      121 raaltqifse yqwdapnlss pvkwkskrkr tcmnasyedv nyifnispcp rtdddvaafl
      181 dgshtadqdv adnilpahvr rlwlasasak ffwidttple sdavtrgaaa spklrsaita
      241 lggdvipitt lvaagsvfqs sdgrtealgp lsaipfsdva esylsgattk svqrttlvvy
      301 sgtnigsavl canssrmlee piaaedwkei rimcrtrqda rlcifqtsrl qifrcvsagm
      361 rtysktpasa lsellsclvn dnsaffvelt lnhsclrhyg flfpldltsf cvhlitepgp
      421 ltsnaflqnf gnefadvsrr evgfapqskt igsvtekpfg anvlekwfvp asintliels
      481 gmqeqstsre cmlqmlrrey emkapiskdk gehrvmsdqp trpasvrnmr kcvsvslpvl
      541 nsatiesaen tslftkretv pitnpccdid qlvmhlkqcy daaldselpv swltcaqniv
      601 svvkrfaesq esqlssgeia ckllrdhftl gcvqiaekya clkdddnlkr rvreyqlqal
      661 laleeevnfq pagncvekvt sllrtlsfty spvsaseflk gvvscsylan lkdiiveige
      721 elnvslsteh nsalasegdf fqlgsvqsvq sqesylssip sslnfqrgtd nptnaveska
      781 marvdlsqps vpekrqivvk qtsskrraid pevprkrekr sktdnaqvne naldgaklqk
      841 svlstpkakr rhvlkttfvp etphgkqgrk avqrrqellr kkfsvtvalp vveetpekkc
      901 lsrpttsksq tpvkyatrdm lsiakvqrtq ssttlkrgfc pkvlfsrfqs tppgsnssec
      961 lfssptsnts pcaldqtprs ektpkklnvc sgselsesse qqcfltprrk vskkllssps
     1021 tpklvlshln aveaspaakr vlsnspvaal grmtsltkte rprrlfnvks cecspgkvva
     1081 lgsaataqtg fdgqlcsata gqslcvsndn vmittsesse tslgspvlpe ctsrftrsks
     1141 retgltphel fvlsqtspvn ngshaskker grkmvgcaml kntsqtprqr vakkvsytpp
     1201 salsllhlts spmllkksn
//
LOCUS       XP_077504184             366 aa            linear   INV 25-NOV-2025
DEFINITION  EEIG family member 2 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504184
VERSION     XP_077504184.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648058.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..366
                     /product="EEIG family member 2 isoform X1"
                     /calculated_mol_wt=39803
     Region          6..151
                     /region_name="NT-C2"
                     /note="N-terminal C2 in EEIG1 and EHBP1 proteins;
                     pfam10358"
                     /db_xref="CDD:463058"
     CDS             1..366
                     /gene="LOC144114372"
                     /coded_by="XM_077648058.1:210..1310"
                     /db_xref="GeneID:144114372"
ORIGIN      
        1 mtfmlkkkky kfqvdiriee ltavpfvnai lfakirlldg gnfsetssre evcnhcvkwd
       61 skfvftckms anantgeldp cicrisirke vkggrsfqkl gfadlnlvef agaglcsrry
      121 llegydakhr qdnsilqlsl smtllsgdpc fkaptlpcpa pseqqpselh vvapkvddhs
      181 gssiassssg fgslprkqrp tigstsaaeg gnggmgcpep geprreedfe qchsrnssyh
      241 sqhsrgsgyg slqshsrhgs sesghvrnls sgssysdtfp gslkppdrkr piskvqnlly
      301 kqiteehrmd strvnadqli edlirdtnle aepaegqglq lfigkdgvpa lktgtasnlk
      361 aatscd
//
LOCUS       XP_077504185             366 aa            linear   INV 25-NOV-2025
DEFINITION  EEIG family member 2 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504185
VERSION     XP_077504185.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648059.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..366
                     /product="EEIG family member 2 isoform X1"
                     /calculated_mol_wt=39803
     Region          6..151
                     /region_name="NT-C2"
                     /note="N-terminal C2 in EEIG1 and EHBP1 proteins;
                     pfam10358"
                     /db_xref="CDD:463058"
     CDS             1..366
                     /gene="LOC144114372"
                     /coded_by="XM_077648059.1:1607..2707"
                     /db_xref="GeneID:144114372"
ORIGIN      
        1 mtfmlkkkky kfqvdiriee ltavpfvnai lfakirlldg gnfsetssre evcnhcvkwd
       61 skfvftckms anantgeldp cicrisirke vkggrsfqkl gfadlnlvef agaglcsrry
      121 llegydakhr qdnsilqlsl smtllsgdpc fkaptlpcpa pseqqpselh vvapkvddhs
      181 gssiassssg fgslprkqrp tigstsaaeg gnggmgcpep geprreedfe qchsrnssyh
      241 sqhsrgsgyg slqshsrhgs sesghvrnls sgssysdtfp gslkppdrkr piskvqnlly
      301 kqiteehrmd strvnadqli edlirdtnle aepaegqglq lfigkdgvpa lktgtasnlk
      361 aatscd
//
LOCUS       XP_077504186             365 aa            linear   INV 25-NOV-2025
DEFINITION  EEIG family member 2 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504186
VERSION     XP_077504186.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648060.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..365
                     /product="EEIG family member 2 isoform X2"
                     /calculated_mol_wt=39732
     Region          6..151
                     /region_name="NT-C2"
                     /note="N-terminal C2 in EEIG1 and EHBP1 proteins;
                     pfam10358"
                     /db_xref="CDD:463058"
     CDS             1..365
                     /gene="LOC144114372"
                     /coded_by="XM_077648060.1:210..1307"
                     /db_xref="GeneID:144114372"
ORIGIN      
        1 mtfmlkkkky kfqvdiriee ltavpfvnai lfakirlldg gnfsetssre evcnhcvkwd
       61 skfvftckms anantgeldp cicrisirke vkggrsfqkl gfadlnlvef agaglcsrry
      121 llegydakhr qdnsilqlsl smtllsgdpc fkaptlpcpa pseqqpselh vvapkvddhs
      181 gssiassssg fgslprkqrp tigstsaegg nggmgcpepg eprreedfeq chsrnssyhs
      241 qhsrgsgygs lqshsrhgss esghvrnlss gssysdtfpg slkppdrkrp iskvqnllyk
      301 qiteehrmds trvnadqlie dlirdtnlea epaegqglql figkdgvpal ktgtasnlka
      361 atscd
//
LOCUS       XP_077504187             364 aa            linear   INV 25-NOV-2025
DEFINITION  EEIG family member 2 isoform X3 [Amblyomma americanum].
ACCESSION   XP_077504187
VERSION     XP_077504187.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648061.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..364
                     /product="EEIG family member 2 isoform X3"
                     /calculated_mol_wt=39547
     Region          6..151
                     /region_name="NT-C2"
                     /note="N-terminal C2 in EEIG1 and EHBP1 proteins;
                     pfam10358"
                     /db_xref="CDD:463058"
     CDS             1..364
                     /gene="LOC144114372"
                     /coded_by="XM_077648061.1:211..1305"
                     /db_xref="GeneID:144114372"
ORIGIN      
        1 mtfmlkkkky kfqvdiriee ltavpfvnai lfakirlldg gnfsetssre evcnhcvkwd
       61 skfvftckms anantgeldp cicrisirke vkggrsfqkl gfadlnlvef agaglcsrry
      121 llegydakhr qdnsilqlsl smtllsgdpc fkaptlpcpa pseqqpselh vvapkvddhs
      181 gssiassssg fgslprkqrp tigstsaaeg gnggmgcpep geprreedfe qchsrnssyh
      241 sqhsrgsgyg slqshsrhgs sesghvrnls sgssysdtfp gslkppdrkr piskvqnlly
      301 iteehrmdst rvnadqlied lirdtnleae paegqglqlf igkdgvpalk tgtasnlkaa
      361 tscd
//
LOCUS       XP_077504188             360 aa            linear   INV 25-NOV-2025
DEFINITION  early estrogen-induced gene 1 protein isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077504188
VERSION     XP_077504188.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648062.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..360
                     /product="early estrogen-induced gene 1 protein isoform
                     X4"
                     /calculated_mol_wt=39072
     Region          6..151
                     /region_name="NT-C2"
                     /note="N-terminal C2 in EEIG1 and EHBP1 proteins;
                     pfam10358"
                     /db_xref="CDD:463058"
     CDS             1..360
                     /gene="LOC144114372"
                     /coded_by="XM_077648062.1:210..1292"
                     /db_xref="GeneID:144114372"
ORIGIN      
        1 mtfmlkkkky kfqvdiriee ltavpfvnai lfakirlldg gnfsetssre evcnhcvkwd
       61 skfvftckms anantgeldp cicrisirke vkggrsfqkl gfadlnlvef agaglcsrry
      121 llegydakhr qdnsilqlsl smtllsgdpc fkaptlpcpa pseqqpselh vvapkvddhs
      181 gssiassssg fgslprkqrp tigstsaaeg gnggmgcpep geprreedfe qchsrnssyh
      241 sqhsrgsgyg slqshsrhgs sesghvrnls sgssysdtfp gslkppdrkr piskkqitee
      301 hrmdstrvna dqliedlird tnleaepaeg qglqlfigkd gvpalktgta snlkaatscd
//
LOCUS       XP_077504189             360 aa            linear   INV 25-NOV-2025
DEFINITION  early estrogen-induced gene 1 protein isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077504189
VERSION     XP_077504189.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648063.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..360
                     /product="early estrogen-induced gene 1 protein isoform
                     X4"
                     /calculated_mol_wt=39072
     Region          6..151
                     /region_name="NT-C2"
                     /note="N-terminal C2 in EEIG1 and EHBP1 proteins;
                     pfam10358"
                     /db_xref="CDD:463058"
     CDS             1..360
                     /gene="LOC144114372"
                     /coded_by="XM_077648063.1:1607..2689"
                     /db_xref="GeneID:144114372"
ORIGIN      
        1 mtfmlkkkky kfqvdiriee ltavpfvnai lfakirlldg gnfsetssre evcnhcvkwd
       61 skfvftckms anantgeldp cicrisirke vkggrsfqkl gfadlnlvef agaglcsrry
      121 llegydakhr qdnsilqlsl smtllsgdpc fkaptlpcpa pseqqpselh vvapkvddhs
      181 gssiassssg fgslprkqrp tigstsaaeg gnggmgcpep geprreedfe qchsrnssyh
      241 sqhsrgsgyg slqshsrhgs sesghvrnls sgssysdtfp gslkppdrkr piskkqitee
      301 hrmdstrvna dqliedlird tnleaepaeg qglqlfigkd gvpalktgta snlkaatscd
//
LOCUS       XP_077504190             359 aa            linear   INV 25-NOV-2025
DEFINITION  early estrogen-induced gene 1 protein isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077504190
VERSION     XP_077504190.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648064.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..359
                     /product="early estrogen-induced gene 1 protein isoform
                     X5"
                     /calculated_mol_wt=39001
     Region          6..151
                     /region_name="NT-C2"
                     /note="N-terminal C2 in EEIG1 and EHBP1 proteins;
                     pfam10358"
                     /db_xref="CDD:463058"
     CDS             1..359
                     /gene="LOC144114372"
                     /coded_by="XM_077648064.1:210..1289"
                     /db_xref="GeneID:144114372"
ORIGIN      
        1 mtfmlkkkky kfqvdiriee ltavpfvnai lfakirlldg gnfsetssre evcnhcvkwd
       61 skfvftckms anantgeldp cicrisirke vkggrsfqkl gfadlnlvef agaglcsrry
      121 llegydakhr qdnsilqlsl smtllsgdpc fkaptlpcpa pseqqpselh vvapkvddhs
      181 gssiassssg fgslprkqrp tigstsaegg nggmgcpepg eprreedfeq chsrnssyhs
      241 qhsrgsgygs lqshsrhgss esghvrnlss gssysdtfpg slkppdrkrp iskkqiteeh
      301 rmdstrvnad qliedlirdt nleaepaegq glqlfigkdg vpalktgtas nlkaatscd
//
LOCUS       XP_077504191             356 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114374 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504191
VERSION     XP_077504191.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648065.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..356
                     /product="uncharacterized protein LOC144114374 isoform X1"
                     /calculated_mol_wt=38274
     Region          <200..338
                     /region_name="NPDC1"
                     /note="Neural proliferation differentiation control-1
                     protein (NPDC1); pfam06809"
                     /db_xref="CDD:284276"
     CDS             1..356
                     /gene="LOC144114374"
                     /coded_by="XM_077648065.1:132..1202"
                     /db_xref="GeneID:144114374"
ORIGIN      
        1 mkrhtpwial lllcviaevr srnyrgplwp lrsgallern qrlrpswtse ggwsepeerl
       61 aeeeqsslkg shllsdreap qlrqrflsen avsaeaagsd vsgleslvdq saveaesrfa
      121 gvsssregad kgvsqaaans seeavvasgp qagalragpn dglhqvtrks asvisdiyfv
      181 alvagcsava vfgvvaagyc fykvqknara aadvdypayg vtgpnkegas ptadrklaqs
      241 aqmyhfqhqk qqmiavekcm tmmplgvlpt glfsssvwlg gvprnhtnrh tsasdvdtee
      301 eeneegdytv yecpgmasgg elevknplfq ddhpivlatp edlgktgdde rkrrsp
//
LOCUS       XP_077504192             351 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114374 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504192
VERSION     XP_077504192.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648066.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..351
                     /product="uncharacterized protein LOC144114374 isoform X2"
                     /calculated_mol_wt=37683
     Region          <195..333
                     /region_name="NPDC1"
                     /note="Neural proliferation differentiation control-1
                     protein (NPDC1); pfam06809"
                     /db_xref="CDD:284276"
     CDS             1..351
                     /gene="LOC144114374"
                     /coded_by="XM_077648066.1:139..1194"
                     /db_xref="GeneID:144114374"
ORIGIN      
        1 mkrhtpwial lllcviaevr srnyrgplwp lrsgallern qrlrpswtse ggwsepeerl
       61 aeeeqsslkg shllsdreap qlrqrflsen avsaeaagsd vsgleslvdq saveagvsss
      121 regadkgvsq aaansseeav vasgpqagal ragpndglhq vtrksasvis diyfvalvag
      181 csavavfgvv aagycfykvq knaraaadvd ypaygvtgpn kegasptadr klaqsaqmyh
      241 fqhqkqqmia vekcmtmmpl gvlptglfss svwlggvprn htnrhtsasd vdteeeenee
      301 gdytvyecpg masggelevk nplfqddhpi vlatpedlgk tgdderkrrs p
//
LOCUS       XP_077504193             330 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114374 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504193
VERSION     XP_077504193.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648067.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..330
                     /product="uncharacterized protein LOC144114374 isoform X3"
                     /calculated_mol_wt=35554
     Region          <132..314
                     /region_name="NPDC1"
                     /note="Neural proliferation differentiation control-1
                     protein (NPDC1); pfam06809"
                     /db_xref="CDD:284276"
     CDS             1..330
                     /gene="LOC144114374"
                     /coded_by="XM_077648067.1:130..1122"
                     /db_xref="GeneID:144114374"
ORIGIN      
        1 mkrhtpwial lllcviaevr srnyrgplwp lrsgallern qrlrpswtse ggwsepeerl
       61 aeeeqsslkg shllsdreap qlrqrflsen avsaeaagsd vsgleslvdq saveaesrfa
      121 gvsssregad kgvsqaaans seeavvasgp qagalragpn dglhqvtrks asvisdiyfv
      181 alvagcsava vfgvvaagyc fykvqknara aadvdypayg vtgpnkegas ptadrklaqs
      241 aqmyhfqhqk qqmiaveknh tnrhtsasdv dteeeeneeg dytvyecpgm asggelevkn
      301 plfqddhpiv latpedlgkt gdderkrrsp
//
LOCUS       XP_077504194             325 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114374 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077504194
VERSION     XP_077504194.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648068.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..325
                     /product="uncharacterized protein LOC144114374 isoform X4"
                     /calculated_mol_wt=34964
     Region          <195..309
                     /region_name="NPDC1"
                     /note="Neural proliferation differentiation control-1
                     protein (NPDC1); pfam06809"
                     /db_xref="CDD:284276"
     CDS             1..325
                     /gene="LOC144114374"
                     /coded_by="XM_077648068.1:132..1109"
                     /db_xref="GeneID:144114374"
ORIGIN      
        1 mkrhtpwial lllcviaevr srnyrgplwp lrsgallern qrlrpswtse ggwsepeerl
       61 aeeeqsslkg shllsdreap qlrqrflsen avsaeaagsd vsgleslvdq saveagvsss
      121 regadkgvsq aaansseeav vasgpqagal ragpndglhq vtrksasvis diyfvalvag
      181 csavavfgvv aagycfykvq knaraaadvd ypaygvtgpn kegasptadr klaqsaqmyh
      241 fqhqkqqmia veknhtnrht sasdvdteee eneegdytvy ecpgmasgge levknplfqd
      301 dhpivlatpe dlgktgdder krrsp
//
LOCUS       XP_077504195             282 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114375 [Amblyomma americanum].
ACCESSION   XP_077504195
VERSION     XP_077504195.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648069.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..282
                     /product="uncharacterized protein LOC144114375"
                     /calculated_mol_wt=30705
     Region          27..136
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     Site            order(31..34,62..63,66,85,122)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260008"
     CDS             1..282
                     /gene="LOC144114375"
                     /coded_by="XM_077648069.1:1..849"
                     /db_xref="GeneID:144114375"
ORIGIN      
        1 mtrddhsgrr laraealary ygnkrgvfyv datgphhggw ytaavvhqnt avnsltskaq
       61 dithaeevai alagadqdsr viitdsrgac rniergfipy fayrilqhsn ylgvpasrti
      121 vwtpahtglk gnetadaaar altlraspsp ppdadsdpnp afafkeiiky yhfghaifpk
      181 pckgltkaee hlllrlytkt llcpavikhf dpactgecph crekssdiyh mvwacqstph
      241 lphipcptre dweaallgcs dlasqkalvg raraaatang ll
//
LOCUS       XP_077504196             222 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114376 [Amblyomma americanum].
ACCESSION   XP_077504196
VERSION     XP_077504196.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648070.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..222
                     /product="uncharacterized protein LOC144114376"
                     /calculated_mol_wt=24605
     Region          93..>195
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..222
                     /gene="LOC144114376"
                     /coded_by="XM_077648070.1:2643..3311"
                     /db_xref="GeneID:144114376"
ORIGIN      
        1 mqrehtnmre airvekrigi gffklcsaae drvvaivfgv gqstvnticr efagtlvdvi
       61 kpewvrmmap nemdehisef yamtgfpqav galdgchfpv ssphlnamdy rnykgwysvi
      121 llalvdhkyr fryvsagspg rchdchvysq sdlaetvegp lfktpvtvin gtavppiilc
      181 dqafaltgnl mkpfphdgir kkdcsiiicl kpdgllkmpl ge
//
LOCUS       XP_077504197             124 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114377 [Amblyomma americanum].
ACCESSION   XP_077504197
VERSION     XP_077504197.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648071.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..124
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..124
                     /product="uncharacterized protein LOC144114377"
                     /calculated_mol_wt=13154
     CDS             1..124
                     /gene="LOC144114377"
                     /coded_by="XM_077648071.1:1..375"
                     /db_xref="GeneID:144114377"
ORIGIN      
        1 mqvsraalkk lprrleldgt eekcqleetw sqlerfvgae pesiciddfv ggddstgkvt
       61 eltdvviaae vtaewpnkda aevdpasadv aplptateav aalalvrryc gaiegiglsl
      121 vdrl
//
LOCUS       XP_077504198             231 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114380 [Amblyomma americanum].
ACCESSION   XP_077504198
VERSION     XP_077504198.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648072.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..231
                     /product="uncharacterized protein LOC144114380"
                     /calculated_mol_wt=26273
     Region          120..197
                     /region_name="DUF4602"
                     /note="Domain of unknown function (DUF4602); pfam15375"
                     /db_xref="CDD:464683"
     CDS             1..231
                     /gene="LOC144114380"
                     /coded_by="XM_077648072.1:127..822"
                     /db_xref="GeneID:144114380"
ORIGIN      
        1 mglssrvlsk lkaygdsfld dsdktaktda sklvskkskc hdrdkrrtcs taspkltkid
       61 rmrieldlgd acderadeqp epprqgsvsv vlfqdpskrk tkkrlseekd vpndvqqsan
      121 eidfkkaryd vlkfgikgle kpkqeaakia lavqlgakpp kkkyvnykel iherkeqkik
      181 dreerivnak tgvkpkrqgn tmhrkthhar hegvhvvske liskvrrkki k
//
LOCUS       XP_077504199             503 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome P450 3A29-like [Amblyomma americanum].
ACCESSION   XP_077504199
VERSION     XP_077504199.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648073.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..503
                     /product="cytochrome P450 3A29-like"
                     /calculated_mol_wt=57550
     Region          67..494
                     /region_name="CYP3A-like"
                     /note="cytochrome P450 family 3, subfamily A and similar
                     cytochrome P450s; cd11055"
                     /db_xref="CDD:410678"
     Site            order(76,104..107,119..120,210,212..213,215,240,304,
                     307..308,312,375,378,486..487)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410678"
     Site            order(104,118..119,126,130,137,305,308..309,312,377,379,
                     438..441,444..448,452)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410678"
     CDS             1..503
                     /gene="LOC144114381"
                     /coded_by="XM_077648073.1:152..1663"
                     /db_xref="GeneID:144114381"
ORIGIN      
        1 masawelwna avlvavvava lawltrrrqq glftrlgypg pkpdliwgsw kqlqrnriqv
       61 mqewidkygk vfgfylakep ymvitdtemi recfvkdsha fqdrpmysld iepfassllf
      121 ikgetwkkvr svlnqgftan kvklifdtvs kcsdvflnel hdqcrkgdva evyhraqrls
      181 ldiitktala wevdcqrqsd dpvlsgmqrl fqdadkvafe safafpgvra ilrylypltt
      241 fsrcvkkmmd dvdttarlrr agqrprsndi lqmildaqtg dkdgehaygr kviedryiis
      301 nciillmagf dttsaalaft mylvakhpee qarileevea rfpgeaelsf dqlhqlerld
      361 avvkeslrly psvpimvmrq caqdttvlgr fipagvtvva ppwhihhdpq lwpepdefrp
      421 drflgegsnq rhiatyfpfg lgqktcigkr vallslkttf mkvlraykld iceqtqepmr
      481 vivpnlvlnp eigvhlrftp rea
//
LOCUS       XP_077504200             503 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome P450 3A29-like [Amblyomma americanum].
ACCESSION   XP_077504200
VERSION     XP_077504200.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648074.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..503
                     /product="cytochrome P450 3A29-like"
                     /calculated_mol_wt=57550
     Region          67..494
                     /region_name="CYP3A-like"
                     /note="cytochrome P450 family 3, subfamily A and similar
                     cytochrome P450s; cd11055"
                     /db_xref="CDD:410678"
     Site            order(76,104..107,119..120,210,212..213,215,240,304,
                     307..308,312,375,378,486..487)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410678"
     Site            order(104,118..119,126,130,137,305,308..309,312,377,379,
                     438..441,444..448,452)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410678"
     CDS             1..503
                     /gene="LOC144114381"
                     /coded_by="XM_077648074.1:179..1690"
                     /db_xref="GeneID:144114381"
ORIGIN      
        1 masawelwna avlvavvava lawltrrrqq glftrlgypg pkpdliwgsw kqlqrnriqv
       61 mqewidkygk vfgfylakep ymvitdtemi recfvkdsha fqdrpmysld iepfassllf
      121 ikgetwkkvr svlnqgftan kvklifdtvs kcsdvflnel hdqcrkgdva evyhraqrls
      181 ldiitktala wevdcqrqsd dpvlsgmqrl fqdadkvafe safafpgvra ilrylypltt
      241 fsrcvkkmmd dvdttarlrr agqrprsndi lqmildaqtg dkdgehaygr kviedryiis
      301 nciillmagf dttsaalaft mylvakhpee qarileevea rfpgeaelsf dqlhqlerld
      361 avvkeslrly psvpimvmrq caqdttvlgr fipagvtvva ppwhihhdpq lwpepdefrp
      421 drflgegsnq rhiatyfpfg lgqktcigkr vallslkttf mkvlraykld iceqtqepmr
      481 vivpnlvlnp eigvhlrftp rea
//
LOCUS       XP_077504201             566 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylserine lipase ABHD16A isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504201
VERSION     XP_077504201.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648075.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..566
                     /product="phosphatidylserine lipase ABHD16A isoform X1"
                     /calculated_mol_wt=63230
     Region          260..416
                     /region_name="FrsA"
                     /note="Fermentation-respiration switch esterase FrsA,
                     DUF1100 family [Signal transduction mechanisms]; COG1073"
                     /db_xref="CDD:440691"
     CDS             1..566
                     /gene="LOC144114382"
                     /coded_by="XM_077648075.1:106..1806"
                     /db_xref="GeneID:144114382"
ORIGIN      
        1 mkmagsfifk yiygprlyri hktadregrs yepigmerys dtlirsvsfa lslawsigvy
       61 tspiiaavly rrgyvtfegv yaltrlagai cvvmvgticl rgigrisssd ygqflralqa
      121 aqqnlskesk melakydsef yawpidfrwn dplvdkskpr vevesqpskr gsffstlral
      181 pcevlsyiav htfgrrmmyp gtvkllqaav apmliqgrar lieehnairh klltrdgnei
      241 dsvfidrrns tlyangnilv vccegnagfy eygiaatpie agysvlgwnh pgfagstgvp
      301 fpdqeknavd vvmqfaihrl gfapediivy awsigaycat waamnypdik glildatfdd
      361 vlplaaskmp pswepivrrt irgylnlnnl eqlhryggpv llyrrtrdei istqesaqiv
      421 snrgndllig flrhrfpklv teetlwalrd wlsgdrghqm vlwneggvde eacmdklavy
      481 irdngpsfpm ligdglnvdv kiqlilflar khmvdfnsth ctplpsglfq vpwdlvqavd
      541 lygrgadgfc rvsadmcgpp psgdap
//
LOCUS       XP_077504202             562 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylserine lipase ABHD16A isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504202
VERSION     XP_077504202.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648076.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..562
                     /product="phosphatidylserine lipase ABHD16A isoform X2"
                     /calculated_mol_wt=62826
     Region          256..412
                     /region_name="FrsA"
                     /note="Fermentation-respiration switch esterase FrsA,
                     DUF1100 family [Signal transduction mechanisms]; COG1073"
                     /db_xref="CDD:440691"
     CDS             1..562
                     /gene="LOC144114382"
                     /coded_by="XM_077648076.1:106..1794"
                     /db_xref="GeneID:144114382"
ORIGIN      
        1 mkmagsfifk yiygprlyri hktadregrs yepigmerys dtlirslsla wsigvytspi
       61 iaavlyrrgy vtfegvyalt rlagaicvvm vgticlrgig risssdygqf lralqaaqqn
      121 lskeskmela kydsefyawp idfrwndplv dkskprveve sqpskrgsff stlralpcev
      181 lsyiavhtfg rrmmypgtvk llqaavapml iqgrarliee hnairhkllt rdgneidsvf
      241 idrrnstlya ngnilvvcce gnagfyeygi aatpieagys vlgwnhpgfa gstgvpfpdq
      301 eknavdvvmq faihrlgfap ediivyawsi gaycatwaam nypdikglil datfddvlpl
      361 aaskmppswe pivrrtirgy lnlnnleqlh ryggpvllyr rtrdeiistq esaqivsnrg
      421 ndlligflrh rfpklvteet lwalrdwlsg drghqmvlwn eggvdeeacm dklavyirdn
      481 gpsfpmligd glnvdvkiql ilflarkhmv dfnsthctpl psglfqvpwd lvqavdlygr
      541 gadgfcrvsa dmcgpppsgd ap
//
LOCUS       XP_077504203             553 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylserine lipase ABHD16A isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504203
VERSION     XP_077504203.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648077.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..553
                     /product="phosphatidylserine lipase ABHD16A isoform X3"
                     /calculated_mol_wt=62137
     Region          260..416
                     /region_name="FrsA"
                     /note="Fermentation-respiration switch esterase FrsA,
                     DUF1100 family [Signal transduction mechanisms]; COG1073"
                     /db_xref="CDD:440691"
     CDS             1..553
                     /gene="LOC144114382"
                     /coded_by="XM_077648077.1:106..1767"
                     /db_xref="GeneID:144114382"
ORIGIN      
        1 mkmagsfifk yiygprlyri hktadregrs yepigmerys dtlirsvsfa lslawsigvy
       61 tspiiaavly rrgyvtfegv yaltrlagai cvvmvgticl rgigrisssd ygqflralqa
      121 aqqnlskesk melakydsef yawpidfrwn dplvdkskpr vevesqpskr gsffstlral
      181 pcevlsyiav htfgrrmmyp gtvkllqaav apmliqgrar lieehnairh klltrdgnei
      241 dsvfidrrns tlyangnilv vccegnagfy eygiaatpie agysvlgwnh pgfagstgvp
      301 fpdqeknavd vvmqfaihrl gfapediivy awsigaycat waamnypdik glildatfdd
      361 vlplaaskmp pswepivrrt irgylnlnnl eqlhryggpv llyrrtrdei istqesaqiv
      421 snrgndllig flrhrfpklv teetlwalrd wlsgdrghqm vlwneggvde eacmdklavy
      481 irdngpsfpm ligdglnvdv kiqlilflar khmvdfnsth ctplpsglfq vpwdlvqavd
      541 lygregtppr arm
//
LOCUS       XP_077504204             549 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylserine lipase ABHD16A isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077504204
VERSION     XP_077504204.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648078.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..549
                     /product="phosphatidylserine lipase ABHD16A isoform X4"
                     /calculated_mol_wt=61732
     Region          256..412
                     /region_name="FrsA"
                     /note="Fermentation-respiration switch esterase FrsA,
                     DUF1100 family [Signal transduction mechanisms]; COG1073"
                     /db_xref="CDD:440691"
     CDS             1..549
                     /gene="LOC144114382"
                     /coded_by="XM_077648078.1:86..1735"
                     /db_xref="GeneID:144114382"
ORIGIN      
        1 mkmagsfifk yiygprlyri hktadregrs yepigmerys dtlirslsla wsigvytspi
       61 iaavlyrrgy vtfegvyalt rlagaicvvm vgticlrgig risssdygqf lralqaaqqn
      121 lskeskmela kydsefyawp idfrwndplv dkskprveve sqpskrgsff stlralpcev
      181 lsyiavhtfg rrmmypgtvk llqaavapml iqgrarliee hnairhkllt rdgneidsvf
      241 idrrnstlya ngnilvvcce gnagfyeygi aatpieagys vlgwnhpgfa gstgvpfpdq
      301 eknavdvvmq faihrlgfap ediivyawsi gaycatwaam nypdikglil datfddvlpl
      361 aaskmppswe pivrrtirgy lnlnnleqlh ryggpvllyr rtrdeiistq esaqivsnrg
      421 ndlligflrh rfpklvteet lwalrdwlsg drghqmvlwn eggvdeeacm dklavyirdn
      481 gpsfpmligd glnvdvkiql ilflarkhmv dfnsthctpl psglfqvpwd lvqavdlygr
      541 egtpprarm
//
LOCUS       XP_077504205            1465 aa            linear   INV 25-NOV-2025
DEFINITION  RNA helicase aquarius [Amblyomma americanum].
ACCESSION   XP_077504205
VERSION     XP_077504205.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648079.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1465
                     /product="RNA helicase aquarius"
                     /calculated_mol_wt=168817
     Region          13..808
                     /region_name="Aquarius_N"
                     /note="Intron-binding protein aquarius N-terminus;
                     pfam16399"
                     /db_xref="CDD:435319"
     Region          803..1157
                     /region_name="EEXXQc_AQR"
                     /note="EEXXQ-box helicase domain of AQR; cd17935"
                     /db_xref="CDD:350693"
     Site            order(806..809,812,831..837,867,1143..1144)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350693"
     Region          1145..1329
                     /region_name="SF1_C_Upf1"
                     /note="C-terminal helicase domain of Upf1-like family
                     helicases; cd18808"
                     /db_xref="CDD:350195"
     Site            order(1235..1237,1265,1267..1268,1283)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:350195"
     Site            order(1271,1273,1302)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350195"
     CDS             1..1465
                     /gene="LOC144114383"
                     /coded_by="XM_077648079.1:15..4412"
                     /db_xref="GeneID:144114383"
ORIGIN      
        1 maafsslsrs gtadtitkla edywvphnks swreydpkvi vdiyqkeiig sgfairkvml
       61 lefsqyleny lwshynpqda stahlmsivv mvnekfrerv pawptfiarp dhfpsffrkv
      121 mesaldenfs fslqeqtall vflincfnsm evslireqiq rlvslsawrt llperrdyef
      181 krfpklkkfw ayidkndkkl deqayknlly ereflsrlig rffkylesvp takdvpeldk
      241 pamevvhyce rflelmtdle ahlptrrffn timdnshlvv kcrlsalcqr negrlftqll
      301 drlnfysrfe isdqtgeelt dhdmmqihyd nitalqkaaf kdfidlrrfs lsnvanvdtr
      361 ekllkhfgsl ktedlhrlaa slflvppmns eskespysht fleellisrh errqsqlesl
      421 nemplyptes iiwdenvvps eyfsgegcla lpklnlqflt lhdyllrnfh lfrlestyei
      481 rqdiedavsr lkpwktedeg vmfggwarma qpivsfaive vgkpnigekq pskvradvtv
      541 hlnvrreika ewealrkhdv cflitvrpac ppgtlynyke pfvpqvglty vrgcevegll
      601 dlngrvieeg pepkpvlpge trtyrvwldc nqykidmdhn vkggedvydt fnilmrrkpk
      661 ennfkavlet irdlmntecv vpdwlhdivl gygdpgaahy skmsnqiptl nfndtflsmd
      721 hlrtsfehkc disvdvkdpr llvppfkvtf cdvalqqqta lkqgsrkgkd gagpdkpark
      781 ekilvtphit eskgpysgew tkknaipftp tqieairagl qpgltiivgp pgtgktdvav
      841 qiisnlyhnf peqrtllvth snqalnqlfe kimmldider hllrlghgee aletekdfsr
      901 ygrvnyvlgk rlellqevsr lqdslgvsgd vsytcetagf fylyqvlsrw eeflskvkpg
      961 aatkplaqdi qdhfpfakff snapqplfhg qsfeddmeka qscfryikdi fdqleefraf
     1021 ellrsgldrs ryllvkeakv iamtcthaal krrelvdlgf kydnilmees aqileietfi
     1081 plllqtpqdg ynrlkrwimi gdhhqlppvv knmafqkysn meqslftrfv rlgvpvvqld
     1141 gqgrsrpslc dlynwryqnl gnlphvtewp eyrtgnagfc fdyqlvdvgd fngigesepn
     1201 pyfyqnlaea eyvvavfmym rllgypaeri silttyngqk hlirdvvrqr cqdnpligcp
     1261 lkvttvdkyq gqqndyvlls lvrtravghl rdvrrlvvam srarlglyvf arislfqdcf
     1321 elgptfnlll qrptqlwlap weayptarpn nvpaqgepyv itgmtqmaqf vydfyqqrld
     1381 altaqlkaqk avyekppmti kevpkeipke igedelaape sqpveamdve atgtgdagst
     1441 pstqhpsavl evqemksdss dseer
//
LOCUS       XP_077504206             489 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-L-fucosidase-like [Amblyomma americanum].
ACCESSION   XP_077504206
VERSION     XP_077504206.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648080.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..489
                     /product="alpha-L-fucosidase-like"
                     /calculated_mol_wt=56170
     Region          21..405
                     /region_name="Alpha_L_fucos"
                     /note="Alpha-L-fucosidase; smart00812"
                     /db_xref="CDD:214829"
     Region          369..456
                     /region_name="Fucosidase_C"
                     /note="Alpha-L-fucosidase C-terminal domain; pfam16757"
                     /db_xref="CDD:465259"
     CDS             1..489
                     /gene="LOC144114384"
                     /coded_by="XM_077648080.1:117..1586"
                     /db_xref="GeneID:144114384"
ORIGIN      
        1 mvqrwlvplv avlvavapsw aaarymptwd slderplpaw fdeakvgifv hwgvfsvpsf
       61 tsewfwwywq gwgeprnpkp akymaenypp gftypdfakd ftceffdpde warilqesga
      121 ryvvltakhh egftlwpsnv sfnwnakdvg pkrdlvgdla nairsktnlv fgvyhslfew
      181 fnplfledkk nnfttsyfse gktlvelrel veryrpeivw sdgdpapaey wksreflawl
      241 yndspvkdtv vtndrwcqgc qckhggyftc ddrynpgvlq phkwencmtl drqswgyrrd
      301 aaltdifsiq elittlvesi scggnilinv gptrdgrlel iyqerlqqlg rwlgvngeav
      361 ygstawtcqn dtaaphvwyt qgrdqavyvf vlrwppqgll ilgslrldrd srvvllgaeg
      421 epvsvlaqta sllilqlppl tvdrlptpwa walrvtgpas ghcpeqtrgr pktmsyflhe
      481 krerpvwsp
//
LOCUS       XP_077504207             534 aa            linear   INV 25-NOV-2025
DEFINITION  U4-U6 small nuclear riboprotein factor 60K isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504207
VERSION     XP_077504207.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648081.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..534
                     /product="U4-U6 small nuclear riboprotein factor 60K
                     isoform X1"
                     /calculated_mol_wt=60598
     Region          107..>141
                     /region_name="SFM"
                     /note="Splicing Factor Motif, present in Prp18 and Pr04;
                     smart00500"
                     /db_xref="CDD:128776"
     Region          <240..531
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          245..282
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          288..332
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          338..374
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          381..416
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          422..456
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          464..501
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          507..531
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..534
                     /gene="U4-U6-60K"
                     /coded_by="XM_077648081.1:128..1732"
                     /db_xref="GeneID:144114385"
ORIGIN      
        1 mlqvlvksde eemsddedia yikrqkvvhy gsleekerqr laekdpaven gsdedsqsmd
       61 lkaamasgni hisdeymdie deggrdkqql leeferrkka rqinvstddt evkstlremg
      121 epiclfgegp adrrerlrhl laqlgqdair rkqeeerere relrkdtset twyhesnekl
      181 recriwiaqf slprarhria takeklktqg paasdsqlha krqelyktlr slnincsqig
      241 dtrpisfcef spnsqilata swsglcklwt ipecspirtl rghncnvgai tfhprstylq
      301 sesslnmasc sadgvvnlwn lkseepistl kgmcphrvsr vafhpsgrfl ascvfdhswr
      361 lldlesetev lhqeghskav ydisfqcdgs laatggmdaf grvwdlrtgr cimfldghlk
      421 svlslcfspn gyvlatgsed ntiklwdlrq rkceytipah qnlvsrvife klsgeylvta
      481 sydntvkiws npewtpihsl sghdgkimga dispsgkyla tssfdrtfkl wtpe
//
LOCUS       XP_077504208             522 aa            linear   INV 25-NOV-2025
DEFINITION  U4-U6 small nuclear riboprotein factor 60K isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504208
VERSION     XP_077504208.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648082.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..522
                     /product="U4-U6 small nuclear riboprotein factor 60K
                     isoform X2"
                     /calculated_mol_wt=59197
     Region          95..>129
                     /region_name="SFM"
                     /note="Splicing Factor Motif, present in Prp18 and Pr04;
                     smart00500"
                     /db_xref="CDD:128776"
     Region          <228..519
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          233..270
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          276..320
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          326..362
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          369..404
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          410..444
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          452..489
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          495..519
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..522
                     /gene="U4-U6-60K"
                     /coded_by="XM_077648082.1:200..1768"
                     /db_xref="GeneID:144114385"
ORIGIN      
        1 msddediayi krqkvvhygs leekerqrla ekdpavengs dedsqsmdlk aamasgnihi
       61 sdeymdiede ggrdkqqlle eferrkkarq invstddtev kstlremgep iclfgegpad
      121 rrerlrhlla qlgqdairrk qeeererere lrkdtsettw yhesneklre criwiaqfsl
      181 prarhriata keklktqgpa asdsqlhakr qelyktlrsl nincsqigdt rpisfcefsp
      241 nsqilatasw sglcklwtip ecspirtlrg hncnvgaitf hprstylqse sslnmascsa
      301 dgvvnlwnlk seepistlkg mcphrvsrva fhpsgrflas cvfdhswrll dlesetevlh
      361 qeghskavyd isfqcdgsla atggmdafgr vwdlrtgrci mfldghlksv lslcfspngy
      421 vlatgsednt iklwdlrqrk ceytipahqn lvsrvifekl sgeylvtasy dntvkiwsnp
      481 ewtpihslsg hdgkimgadi spsgkylats sfdrtfklwt pe
//
LOCUS       XP_077504209             522 aa            linear   INV 25-NOV-2025
DEFINITION  U4-U6 small nuclear riboprotein factor 60K isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504209
VERSION     XP_077504209.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648083.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..522
                     /product="U4-U6 small nuclear riboprotein factor 60K
                     isoform X2"
                     /calculated_mol_wt=59197
     Region          95..>129
                     /region_name="SFM"
                     /note="Splicing Factor Motif, present in Prp18 and Pr04;
                     smart00500"
                     /db_xref="CDD:128776"
     Region          <228..519
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          233..270
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          276..320
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          326..362
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          369..404
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          410..444
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          452..489
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          495..519
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..522
                     /gene="U4-U6-60K"
                     /coded_by="XM_077648083.1:136..1704"
                     /db_xref="GeneID:144114385"
ORIGIN      
        1 msddediayi krqkvvhygs leekerqrla ekdpavengs dedsqsmdlk aamasgnihi
       61 sdeymdiede ggrdkqqlle eferrkkarq invstddtev kstlremgep iclfgegpad
      121 rrerlrhlla qlgqdairrk qeeererere lrkdtsettw yhesneklre criwiaqfsl
      181 prarhriata keklktqgpa asdsqlhakr qelyktlrsl nincsqigdt rpisfcefsp
      241 nsqilatasw sglcklwtip ecspirtlrg hncnvgaitf hprstylqse sslnmascsa
      301 dgvvnlwnlk seepistlkg mcphrvsrva fhpsgrflas cvfdhswrll dlesetevlh
      361 qeghskavyd isfqcdgsla atggmdafgr vwdlrtgrci mfldghlksv lslcfspngy
      421 vlatgsednt iklwdlrqrk ceytipahqn lvsrvifekl sgeylvtasy dntvkiwsnp
      481 ewtpihslsg hdgkimgadi spsgkylats sfdrtfklwt pe
//
LOCUS       XP_077504210             902 aa            linear   INV 25-NOV-2025
DEFINITION  proto-oncogene vav-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504210
VERSION     XP_077504210.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648084.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..902
                     /product="proto-oncogene vav-like isoform X1"
                     /calculated_mol_wt=102742
     Region          4..120
                     /region_name="CH_VAV"
                     /note="calponin homology (CH) domain found in VAV
                     proteins; cd21201"
                     /db_xref="CDD:409050"
     Site            order(5,9,72,74..75,78..79,81,92..100,106,108..109,
                     111..112,115..116,119)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409050"
     Region          206..380
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          398..528
                     /region_name="PH_Vav"
                     /note="Vav pleckstrin homology (PH) domain; cd01223"
                     /db_xref="CDD:269930"
     Region          534..585
                     /region_name="C1_VAV"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     found in VAV proteins; cd20810"
                     /db_xref="CDD:410360"
     Region          628..680
                     /region_name="SH3"
                     /note="Src homology 3 domains; smart00326"
                     /db_xref="CDD:214620"
     Site            order(632,634,637,641,659..660,675,677..678)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212690"
     Region          723..822
                     /region_name="SH2"
                     /note="Src homology 2 (SH2) domain; cl15255"
                     /db_xref="CDD:472789"
     Site            order(735,753,774,776)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198193"
     Site            order(775,801)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198193"
     Region          841..894
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     Site            order(845,847,850,854,874..875,888,890..891)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212700"
     CDS             1..902
                     /gene="LOC144114387"
                     /coded_by="XM_077648084.1:238..2946"
                     /db_xref="GeneID:144114387"
ORIGIN      
        1 madvlwrdca nwlircqalp pdhrvtwana qlidlantlr dgvllcqlln klqpgcvdlk
       61 emslrpqmsq flclknirtf lqtcknvfnl nnsdlfepnm lfeygdfrkv lhtlavlsrs
      121 pkaeesgvrg fsdksttshd yyndeafnle davnhnddtm vvfdfpvtpr rtreedvyed
      181 lcyvtlrvne vpavaptapl ekrdycikel ieteknyiea lnmitrdfmr plkdvltpee
      241 neiifkhike ladthtgfha dlhkacissq hlkisdcflr wkdkfivygd ycsnlpkaqe
      301 qidelcskkq lvnqsvlncq qkandgkfkl rdllsvpmqr vlkyhlllke lmkntaethe
      361 dyhglekalq amldlahyin evkrdnetlq iiadiqasit dvdlpqnmel kdygrllkdg
      421 elkirshddn nkiknryifi fdrmmlmcks tkimdklfgg eqyiykssla lcdyrldnap
      481 anarmatryy wllvhvenkt aytmyaktee mkkkwieaie kamdninpsi akftdhqfmm
      541 htfkvptyca dcnkllpgif fqgykctvcg lgvhkvcigt vrscgapslp prpsshlgsq
      601 sstdepqtpp rphadhgang irfsisiaka kqsfsssspg ylsfvaddii evlrkisssi
      661 wegrlqrtte egffpaacve eldkrgsyec paplgrgygs vqqgmqvapp alpsrdgyvn
      721 lnleeypwfa gpmeregats llekspsgtf llristkqng gyaisinfdn qvkhmrvcyd
      781 dnqfylsetk lfrsivelvs wyethslses fkgldislli pyknvvsapi tngtvehlgh
      841 avalhnflpt tesmlglvkg dvvrvlskag qekgwwkgqi qervgyfpwq yvqeipatst
      901 ec
//
LOCUS       XP_077504211             899 aa            linear   INV 25-NOV-2025
DEFINITION  proto-oncogene vav-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504211
VERSION     XP_077504211.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648085.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..899
                     /product="proto-oncogene vav-like isoform X2"
                     /calculated_mol_wt=102321
     Region          4..120
                     /region_name="CH_VAV"
                     /note="calponin homology (CH) domain found in VAV
                     proteins; cd21201"
                     /db_xref="CDD:409050"
     Site            order(5,9,72,74..75,78..79,81,92..100,106,108..109,
                     111..112,115..116,119)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409050"
     Region          203..377
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          395..525
                     /region_name="PH_Vav"
                     /note="Vav pleckstrin homology (PH) domain; cd01223"
                     /db_xref="CDD:269930"
     Region          531..582
                     /region_name="C1_VAV"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     found in VAV proteins; cd20810"
                     /db_xref="CDD:410360"
     Region          625..677
                     /region_name="SH3"
                     /note="Src homology 3 domains; smart00326"
                     /db_xref="CDD:214620"
     Site            order(629,631,634,638,656..657,672,674..675)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212690"
     Region          720..819
                     /region_name="SH2"
                     /note="Src homology 2 (SH2) domain; cl15255"
                     /db_xref="CDD:472789"
     Site            order(732,750,771,773)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198193"
     Site            order(772,798)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198193"
     Region          838..891
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     Site            order(842,844,847,851,871..872,885,887..888)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212700"
     CDS             1..899
                     /gene="LOC144114387"
                     /coded_by="XM_077648085.1:269..2968"
                     /db_xref="GeneID:144114387"
ORIGIN      
        1 madvlwrdca nwlircqalp pdhrvtwana qlidlantlr dgvllcqlln klqpgcvdlk
       61 emslrpqmsq flclknirtf lqtcknvfnl nnsdlfepnm lfeygdfrkv lhtlavlsrs
      121 pkaeesgvrg fsdksttshd yyndeafnle davnneafge fgeyeyeavt nddediyedl
      181 cayrrvqvpa vaptaplekr dycikeliet eknyiealnm itrdfmrplk dvltpeenei
      241 ifkhikelad thtgfhadlh kacissqhlk isdcflrwkd kfivygdycs nlpkaqeqid
      301 elcskkqlvn qsvlncqqka ndgkfklrdl lsvpmqrvlk yhlllkelmk ntaethedyh
      361 glekalqaml dlahyinevk rdnetlqiia diqasitdvd lpqnmelkdy grllkdgelk
      421 irshddnnki knryififdr mmlmckstki mdklfggeqy iyksslalcd yrldnapana
      481 rmatryywll vhvenktayt myakteemkk kwieaiekam dninpsiakf tdhqfmmhtf
      541 kvptycadcn kllpgiffqg ykctvcglgv hkvcigtvrs cgapslpprp sshlgsqsst
      601 depqtpprph adhgangirf sisiakakqs fsssspgyls fvaddiievl rkisssiweg
      661 rlqrtteegf fpaacveeld krgsyecpap lgrgygsvqq gmqvappalp srdgyvnlnl
      721 eeypwfagpm eregatslle kspsgtfllr istkqnggya isinfdnqvk hmrvcyddnq
      781 fylsetklfr sivelvswye thslsesfkg ldisllipyk nvvsapitng tvehlghava
      841 lhnflpttes mlglvkgdvv rvlskagqek gwwkgqiqer vgyfpwqyvq eipatstec
//
LOCUS       XP_077504212             895 aa            linear   INV 25-NOV-2025
DEFINITION  proto-oncogene vav-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077504212
VERSION     XP_077504212.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648086.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..895
                     /product="proto-oncogene vav-like isoform X3"
                     /calculated_mol_wt=101937
     Region          4..120
                     /region_name="CH_VAV"
                     /note="calponin homology (CH) domain found in VAV
                     proteins; cd21201"
                     /db_xref="CDD:409050"
     Site            order(5,9,72,74..75,78..79,81,92..100,106,108..109,
                     111..112,115..116,119)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409050"
     Region          206..380
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          398..521
                     /region_name="PH_Vav"
                     /note="Vav pleckstrin homology (PH) domain; cd01223"
                     /db_xref="CDD:269930"
     Region          527..578
                     /region_name="C1_VAV"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     found in VAV proteins; cd20810"
                     /db_xref="CDD:410360"
     Region          621..673
                     /region_name="SH3"
                     /note="Src homology 3 domains; smart00326"
                     /db_xref="CDD:214620"
     Site            order(625,627,630,634,652..653,668,670..671)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212690"
     Region          716..815
                     /region_name="SH2"
                     /note="Src homology 2 (SH2) domain; cl15255"
                     /db_xref="CDD:472789"
     Site            order(728,746,767,769)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198193"
     Site            order(768,794)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198193"
     Region          834..887
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     Site            order(838,840,843,847,867..868,881,883..884)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212700"
     CDS             1..895
                     /gene="LOC144114387"
                     /coded_by="XM_077648086.1:207..2894"
                     /db_xref="GeneID:144114387"
ORIGIN      
        1 madvlwrdca nwlircqalp pdhrvtwana qlidlantlr dgvllcqlln klqpgcvdlk
       61 emslrpqmsq flclknirtf lqtcknvfnl nnsdlfepnm lfeygdfrkv lhtlavlsrs
      121 pkaeesgvrg fsdksttshd yyndeafnle davnhnddtm vvfdfpvtpr rtreedvyed
      181 lcyvtlrvne vpavaptapl ekrdycikel ieteknyiea lnmitrdfmr plkdvltpee
      241 neiifkhike ladthtgfha dlhkacissq hlkisdcflr wkdkfivygd ycsnlpkaqe
      301 qidelcskkq lvnqsvlncq qkandgkfkl rdllsvpmqr vlkyhlllke lmkntaethe
      361 dyhglekalq amldlahyin evkrdnetlq iiadiqasit dvdlpqnmel kdygrllkdg
      421 elkirshddn nkiknryifi fdrmmlmcks tkgeqyiyks slalcdyrld napanarmat
      481 ryywllvhve nktaytmyak teemkkkwie aiekamdnin psiakftdhq fmmhtfkvpt
      541 ycadcnkllp giffqgykct vcglgvhkvc igtvrscgap slpprpsshl gsqsstdepq
      601 tpprphadhg angirfsisi akakqsfsss spgylsfvad diievlrkis ssiwegrlqr
      661 tteegffpaa cveeldkrgs yecpaplgrg ygsvqqgmqv appalpsrdg yvnlnleeyp
      721 wfagpmereg atslleksps gtfllristk qnggyaisin fdnqvkhmrv cyddnqfyls
      781 etklfrsive lvswyethsl sesfkgldis llipyknvvs apitngtveh lghavalhnf
      841 lpttesmlgl vkgdvvrvls kagqekgwwk gqiqervgyf pwqyvqeipa tstec
//
LOCUS       XP_077504213             892 aa            linear   INV 25-NOV-2025
DEFINITION  proto-oncogene vav-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077504213
VERSION     XP_077504213.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648087.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..892
                     /product="proto-oncogene vav-like isoform X4"
                     /calculated_mol_wt=101516
     Region          4..120
                     /region_name="CH_VAV"
                     /note="calponin homology (CH) domain found in VAV
                     proteins; cd21201"
                     /db_xref="CDD:409050"
     Site            order(5,9,72,74..75,78..79,81,92..100,106,108..109,
                     111..112,115..116,119)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409050"
     Region          203..377
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          395..518
                     /region_name="PH_Vav"
                     /note="Vav pleckstrin homology (PH) domain; cd01223"
                     /db_xref="CDD:269930"
     Region          524..575
                     /region_name="C1_VAV"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     found in VAV proteins; cd20810"
                     /db_xref="CDD:410360"
     Region          618..670
                     /region_name="SH3"
                     /note="Src homology 3 domains; smart00326"
                     /db_xref="CDD:214620"
     Site            order(622,624,627,631,649..650,665,667..668)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212690"
     Region          713..812
                     /region_name="SH2"
                     /note="Src homology 2 (SH2) domain; cl15255"
                     /db_xref="CDD:472789"
     Site            order(725,743,764,766)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198173"
     Site            order(765,791)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198173"
     Region          831..884
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     Site            order(835,837,840,844,864..865,878,880..881)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212700"
     CDS             1..892
                     /gene="LOC144114387"
                     /coded_by="XM_077648087.1:269..2947"
                     /db_xref="GeneID:144114387"
ORIGIN      
        1 madvlwrdca nwlircqalp pdhrvtwana qlidlantlr dgvllcqlln klqpgcvdlk
       61 emslrpqmsq flclknirtf lqtcknvfnl nnsdlfepnm lfeygdfrkv lhtlavlsrs
      121 pkaeesgvrg fsdksttshd yyndeafnle davnneafge fgeyeyeavt nddediyedl
      181 cayrrvqvpa vaptaplekr dycikeliet eknyiealnm itrdfmrplk dvltpeenei
      241 ifkhikelad thtgfhadlh kacissqhlk isdcflrwkd kfivygdycs nlpkaqeqid
      301 elcskkqlvn qsvlncqqka ndgkfklrdl lsvpmqrvlk yhlllkelmk ntaethedyh
      361 glekalqaml dlahyinevk rdnetlqiia diqasitdvd lpqnmelkdy grllkdgelk
      421 irshddnnki knryififdr mmlmckstkg eqyiykssla lcdyrldnap anarmatryy
      481 wllvhvenkt aytmyaktee mkkkwieaie kamdninpsi akftdhqfmm htfkvptyca
      541 dcnkllpgif fqgykctvcg lgvhkvcigt vrscgapslp prpsshlgsq sstdepqtpp
      601 rphadhgang irfsisiaka kqsfsssspg ylsfvaddii evlrkisssi wegrlqrtte
      661 egffpaacve eldkrgsyec paplgrgygs vqqgmqvapp alpsrdgyvn lnleeypwfa
      721 gpmeregats llekspsgtf llristkqng gyaisinfdn qvkhmrvcyd dnqfylsetk
      781 lfrsivelvs wyethslses fkgldislli pyknvvsapi tngtvehlgh avalhnflpt
      841 tesmlglvkg dvvrvlskag qekgwwkgqi qervgyfpwq yvqeipatst ec
//
LOCUS       XP_077504214             187 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114388 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504214
VERSION     XP_077504214.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648088.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="uncharacterized protein LOC144114388 isoform X1"
                     /calculated_mol_wt=20065
     CDS             1..187
                     /gene="LOC144114388"
                     /coded_by="XM_077648088.1:199..762"
                     /db_xref="GeneID:144114388"
ORIGIN      
        1 mgdgpemapq ttsiaagggd dfhnleeiqh llqppphssq vshlppaspt hsslrksskk
       61 kthrkkrqgs vtqsvadsky stnspssstv ndenfvpltd skssatmelt ileaspidsa
      121 diptvlvqpp sttpsltack deqileamqd tdpvpknsff gllplylqpt nmlaifcftc
      181 rqvrghi
//
LOCUS       XP_077504215             187 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114388 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504215
VERSION     XP_077504215.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648089.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="uncharacterized protein LOC144114388 isoform X1"
                     /calculated_mol_wt=20065
     CDS             1..187
                     /gene="LOC144114388"
                     /coded_by="XM_077648089.1:130..693"
                     /db_xref="GeneID:144114388"
ORIGIN      
        1 mgdgpemapq ttsiaagggd dfhnleeiqh llqppphssq vshlppaspt hsslrksskk
       61 kthrkkrqgs vtqsvadsky stnspssstv ndenfvpltd skssatmelt ileaspidsa
      121 diptvlvqpp sttpsltack deqileamqd tdpvpknsff gllplylqpt nmlaifcftc
      181 rqvrghi
//
LOCUS       XP_077504216             187 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114388 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504216
VERSION     XP_077504216.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648090.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="uncharacterized protein LOC144114388 isoform X1"
                     /calculated_mol_wt=20065
     CDS             1..187
                     /gene="LOC144114388"
                     /coded_by="XM_077648090.1:130..693"
                     /db_xref="GeneID:144114388"
ORIGIN      
        1 mgdgpemapq ttsiaagggd dfhnleeiqh llqppphssq vshlppaspt hsslrksskk
       61 kthrkkrqgs vtqsvadsky stnspssstv ndenfvpltd skssatmelt ileaspidsa
      121 diptvlvqpp sttpsltack deqileamqd tdpvpknsff gllplylqpt nmlaifcftc
      181 rqvrghi
//
LOCUS       XP_077504217             173 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114388 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504217
VERSION     XP_077504217.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648091.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..173
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..173
                     /product="uncharacterized protein LOC144114388 isoform X2"
                     /calculated_mol_wt=18408
     CDS             1..173
                     /gene="LOC144114388"
                     /coded_by="XM_077648091.1:199..720"
                     /db_xref="GeneID:144114388"
ORIGIN      
        1 mgdgpemapq ttsiaagggd dfhnleeiqh llqppphssq vshlppaspt hsslrksskk
       61 kthrkkrqgs vtqsvadsky stnspssstv ndenfvpltd skssatmelt ileaspidsa
      121 diptvlvqpp sttpsltack deqileaapp trrtrttlwk lacrqhsavc lcs
//
LOCUS       XP_077504218             680 aa            linear   INV 25-NOV-2025
DEFINITION  rac GTPase-activating protein 1-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504218
VERSION     XP_077504218.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648092.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..680
                     /product="rac GTPase-activating protein 1-like isoform X1"
                     /calculated_mol_wt=76347
     Region          37..126
                     /region_name="DUF4200"
                     /note="Domain of unknown function (DUF4200); pfam13863"
                     /db_xref="CDD:464003"
     Region          338..392
                     /region_name="C1_MgcRacGAP"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     found in male germ cell RacGap (MgcRacGAP) and similar
                     proteins; cd20821"
                     /db_xref="CDD:410371"
     Region          405..594
                     /region_name="RhoGAP_MgcRacGAP"
                     /note="RhoGAP (GTPase-activator protein [GAP] for Rho-like
                     small GTPases) domain present in MgcRacGAP proteins.
                     MgcRacGAP plays an important dual role in cytokinesis: i)
                     it is part of centralspindlin-complex, together with the
                     mitotic kinesin MKLP1, which is...; cd04382"
                     /db_xref="CDD:239847"
     Site            order(441,478,482,549,552..553,580)
                     /site_type="other"
                     /note="putative GTPase interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:239847"
     Site            441
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:239847"
     Site            443
                     /site_type="active"
                     /note="activation site [active]"
                     /db_xref="CDD:239847"
     CDS             1..680
                     /gene="LOC144114389"
                     /coded_by="XM_077648092.1:169..2211"
                     /db_xref="GeneID:144114389"
ORIGIN      
        1 mapvkpkmtk lslvgqfddl crhlaptdtt ieetfleslk nqeecrkrwl atelenqelk
       61 khiaslreeq ivcernlana revlqkevhr rekieeekqn kqhqldlird fimndtelrd
      121 etieklaflk penegkdfda nrlgtinesi gsvlspsgds leisdngtcr rssrsrvkrr
      181 snavhpgerv spvkktkgyd evdqpcanvi tttvtikdgk dfvatsrvft qpstpapsvp
      241 pptspeadqe lpklisgklw yhmhvhhgll kqkleeinlg tlhqhkgqrh kegadrtnac
      301 vprqlsfscs apttpvsqrr grtatpkrsh sagrlmsreh tfcpktviks etcgpcgkri
      361 kfyrtaykcn rcrsvchpec rdkvplpcfp tsqtptqggt agcrgslsdy vpstppmvpa
      421 lvihciqeve krglhdvgly ripgserevr elreqfergk gipnmsradi havcgvlkdf
      481 lrslreclit kslwqtfvda aqlkdgnrmc atweaiqqlp qpnrdtlaal mlhlknvach
      541 eevkmpvtnl arvfaptivg fstadtaavt sllaetelqt qvmlalfavp edywedfldv
      601 esddeirtpk plpvpsskll gpiydcgsnt elytppykrt pascsrltss asaknqrnlt
      661 kgsvfwppqk rtvfyspimk
//
LOCUS       XP_077504219             676 aa            linear   INV 25-NOV-2025
DEFINITION  rac GTPase-activating protein 1-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504219
VERSION     XP_077504219.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648093.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..676
                     /product="rac GTPase-activating protein 1-like isoform X2"
                     /calculated_mol_wt=75827
     Region          37..126
                     /region_name="DUF4200"
                     /note="Domain of unknown function (DUF4200); pfam13863"
                     /db_xref="CDD:464003"
     Region          338..392
                     /region_name="C1_MgcRacGAP"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     found in male germ cell RacGap (MgcRacGAP) and similar
                     proteins; cd20821"
                     /db_xref="CDD:410371"
     Region          405..594
                     /region_name="RhoGAP_MgcRacGAP"
                     /note="RhoGAP (GTPase-activator protein [GAP] for Rho-like
                     small GTPases) domain present in MgcRacGAP proteins.
                     MgcRacGAP plays an important dual role in cytokinesis: i)
                     it is part of centralspindlin-complex, together with the
                     mitotic kinesin MKLP1, which is...; cd04382"
                     /db_xref="CDD:239847"
     Site            order(441,478,482,549,552..553,580)
                     /site_type="other"
                     /note="putative GTPase interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:239847"
     Site            441
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:239847"
     Site            443
                     /site_type="active"
                     /note="activation site [active]"
                     /db_xref="CDD:239847"
     CDS             1..676
                     /gene="LOC144114389"
                     /coded_by="XM_077648093.1:169..2199"
                     /db_xref="GeneID:144114389"
ORIGIN      
        1 mapvkpkmtk lslvgqfddl crhlaptdtt ieetfleslk nqeecrkrwl atelenqelk
       61 khiaslreeq ivcernlana revlqkevhr rekieeekqn kqhqldlird fimndtelrd
      121 etieklaflk penegkdfda nrlgtinesi gsvlspsgds leisdngtcr rssrsrvkrr
      181 snavhpgerv spvkktkgyd evdqpcanvi tttvtikdgk dfvatsrvft qpstpapsvp
      241 pptspeadqe lpklisgklw yhmhvhhgll kqkleeinlg tlhqhkgqrh kegadrtnac
      301 vprqlsfscs apttpvsqrr grtatpkrsh sagrlmsreh tfcpktviks etcgpcgkri
      361 kfyrtaykcn rcrsvchpec rdkvplpcfp tsqtptqggt agcrgslsdy vpstppmvpa
      421 lvihciqeve krglhdvgly ripgserevr elreqfergk gipnmsradi havcgvlkdf
      481 lrslreclit kslwqtfvda aqlkdgnrmc atweaiqqlp qpnrdtlaal mlhlknvach
      541 eevkmpvtnl arvfaptivg fstadtaavt sllaetelqt qvmlalfavp edywedfldv
      601 esddeirtpk plpvpsskll gpiydcgsnt elytppykrt pascsrltss asaknqrnlt
      661 kgppqkrtvf yspimk
//
LOCUS       XP_077504220             651 aa            linear   INV 25-NOV-2025
DEFINITION  rac GTPase-activating protein 1-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504220
VERSION     XP_077504220.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648094.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..651
                     /product="rac GTPase-activating protein 1-like isoform X3"
                     /calculated_mol_wt=73386
     Region          37..112
                     /region_name="DUF4200"
                     /note="Domain of unknown function (DUF4200); pfam13863"
                     /db_xref="CDD:464003"
     Region          309..363
                     /region_name="C1_MgcRacGAP"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     found in male germ cell RacGap (MgcRacGAP) and similar
                     proteins; cd20821"
                     /db_xref="CDD:410371"
     Region          376..565
                     /region_name="RhoGAP_MgcRacGAP"
                     /note="RhoGAP (GTPase-activator protein [GAP] for Rho-like
                     small GTPases) domain present in MgcRacGAP proteins.
                     MgcRacGAP plays an important dual role in cytokinesis: i)
                     it is part of centralspindlin-complex, together with the
                     mitotic kinesin MKLP1, which is...; cd04382"
                     /db_xref="CDD:239847"
     Site            order(412,449,453,520,523..524,551)
                     /site_type="other"
                     /note="putative GTPase interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:239847"
     Site            412
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:239847"
     Site            414
                     /site_type="active"
                     /note="activation site [active]"
                     /db_xref="CDD:239847"
     CDS             1..651
                     /gene="LOC144114389"
                     /coded_by="XM_077648094.1:169..2124"
                     /db_xref="GeneID:144114389"
ORIGIN      
        1 mapvkpkmtk lslvgqfddl crhlaptdtt ieetfleslk nqeecrkrwl atelenqelk
       61 khiaslreeq ivcernlana revlqkevhr rekieeekqn kqhqldlird fimndtelrd
      121 etieklaflk penegkdfda nrssrsrvkr rsnavhpger vspvkktkgy devdqpcanv
      181 itttvtikdg kdfvatsrvf tqpstpapsv ppptspeadq elpklisgkl wyhmhvhhgl
      241 lkqkleeinl gtlhqhkgqr hkegadrtna cvprqlsfsc sapttpvsqr rgrtatpkrs
      301 hsagrlmsre htfcpktvik setcgpcgkr ikfyrtaykc nrcrsvchpe crdkvplpcf
      361 ptsqtptqgg tagcrgslsd yvpstppmvp alvihciqev ekrglhdvgl yripgserev
      421 relreqferg kgipnmsrad ihavcgvlkd flrslrecli tkslwqtfvd aaqlkdgnrm
      481 catweaiqql pqpnrdtlaa lmlhlknvac heevkmpvtn larvfaptiv gfstadtaav
      541 tsllaetelq tqvmlalfav pedywedfld vesddeirtp kplpvpsskl lgpiydcgsn
      601 telytppykr tpascsrlts sasaknqrnl tkgsvfwppq krtvfyspim k
//
LOCUS       XP_077504221             628 aa            linear   INV 25-NOV-2025
DEFINITION  rac GTPase-activating protein 1-like isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077504221
VERSION     XP_077504221.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648095.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..628
                     /product="rac GTPase-activating protein 1-like isoform X4"
                     /calculated_mol_wt=70253
     Region          37..126
                     /region_name="DUF4200"
                     /note="Domain of unknown function (DUF4200); pfam13863"
                     /db_xref="CDD:464003"
     Region          286..340
                     /region_name="C1_MgcRacGAP"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     found in male germ cell RacGap (MgcRacGAP) and similar
                     proteins; cd20821"
                     /db_xref="CDD:410371"
     Region          353..542
                     /region_name="RhoGAP_MgcRacGAP"
                     /note="RhoGAP (GTPase-activator protein [GAP] for Rho-like
                     small GTPases) domain present in MgcRacGAP proteins.
                     MgcRacGAP plays an important dual role in cytokinesis: i)
                     it is part of centralspindlin-complex, together with the
                     mitotic kinesin MKLP1, which is...; cd04382"
                     /db_xref="CDD:239847"
     Site            order(389,426,430,497,500..501,528)
                     /site_type="other"
                     /note="putative GTPase interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:239847"
     Site            389
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:239847"
     Site            391
                     /site_type="active"
                     /note="activation site [active]"
                     /db_xref="CDD:239847"
     CDS             1..628
                     /gene="LOC144114389"
                     /coded_by="XM_077648095.1:169..2055"
                     /db_xref="GeneID:144114389"
ORIGIN      
        1 mapvkpkmtk lslvgqfddl crhlaptdtt ieetfleslk nqeecrkrwl atelenqelk
       61 khiaslreeq ivcernlana revlqkevhr rekieeekqn kqhqldlird fimndtelrd
      121 etieklaflk penegkdfda nrlgtinesi gsvlspsgds leisdngtcr rssrsrvkrr
      181 snavhpgerv spvkktkgyd evdqpcanvi tttvtikdgk dfvatsrvft qpstpapsvp
      241 pptspeadqe lpklisgsap ttpvsqrrgr tatpkrshsa grlmsrehtf cpktvikset
      301 cgpcgkrikf yrtaykcnrc rsvchpecrd kvplpcfpts qtptqggtag crgslsdyvp
      361 stppmvpalv ihciqevekr glhdvglyri pgserevrel reqfergkgi pnmsradiha
      421 vcgvlkdflr slreclitks lwqtfvdaaq lkdgnrmcat weaiqqlpqp nrdtlaalml
      481 hlknvachee vkmpvtnlar vfaptivgfs tadtaavtsl laetelqtqv mlalfavped
      541 ywedfldves ddeirtpkpl pvpsskllgp iydcgsntel ytppykrtpa scsrltssas
      601 aknqrnltkg svfwppqkrt vfyspimk
//
LOCUS       XP_077504222             624 aa            linear   INV 25-NOV-2025
DEFINITION  rac GTPase-activating protein 1-like isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077504222
VERSION     XP_077504222.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648096.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..624
                     /product="rac GTPase-activating protein 1-like isoform X5"
                     /calculated_mol_wt=69733
     Region          37..126
                     /region_name="DUF4200"
                     /note="Domain of unknown function (DUF4200); pfam13863"
                     /db_xref="CDD:464003"
     Region          286..340
                     /region_name="C1_MgcRacGAP"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     found in male germ cell RacGap (MgcRacGAP) and similar
                     proteins; cd20821"
                     /db_xref="CDD:410371"
     Region          353..542
                     /region_name="RhoGAP_MgcRacGAP"
                     /note="RhoGAP (GTPase-activator protein [GAP] for Rho-like
                     small GTPases) domain present in MgcRacGAP proteins.
                     MgcRacGAP plays an important dual role in cytokinesis: i)
                     it is part of centralspindlin-complex, together with the
                     mitotic kinesin MKLP1, which is...; cd04382"
                     /db_xref="CDD:239847"
     Site            order(389,426,430,497,500..501,528)
                     /site_type="other"
                     /note="putative GTPase interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:239847"
     Site            389
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:239847"
     Site            391
                     /site_type="active"
                     /note="activation site [active]"
                     /db_xref="CDD:239847"
     CDS             1..624
                     /gene="LOC144114389"
                     /coded_by="XM_077648096.1:169..2043"
                     /db_xref="GeneID:144114389"
ORIGIN      
        1 mapvkpkmtk lslvgqfddl crhlaptdtt ieetfleslk nqeecrkrwl atelenqelk
       61 khiaslreeq ivcernlana revlqkevhr rekieeekqn kqhqldlird fimndtelrd
      121 etieklaflk penegkdfda nrlgtinesi gsvlspsgds leisdngtcr rssrsrvkrr
      181 snavhpgerv spvkktkgyd evdqpcanvi tttvtikdgk dfvatsrvft qpstpapsvp
      241 pptspeadqe lpklisgsap ttpvsqrrgr tatpkrshsa grlmsrehtf cpktvikset
      301 cgpcgkrikf yrtaykcnrc rsvchpecrd kvplpcfpts qtptqggtag crgslsdyvp
      361 stppmvpalv ihciqevekr glhdvglyri pgserevrel reqfergkgi pnmsradiha
      421 vcgvlkdflr slreclitks lwqtfvdaaq lkdgnrmcat weaiqqlpqp nrdtlaalml
      481 hlknvachee vkmpvtnlar vfaptivgfs tadtaavtsl laetelqtqv mlalfavped
      541 ywedfldves ddeirtpkpl pvpsskllgp iydcgsntel ytppykrtpa scsrltssas
      601 aknqrnltkg ppqkrtvfys pimk
//
LOCUS       XP_077504223            1904 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114391 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504223
VERSION     XP_077504223.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648097.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1904
                     /product="uncharacterized protein LOC144114391 isoform X1"
                     /calculated_mol_wt=218224
     Region          23..>594
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          781..1590
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <1519..>1706
                     /region_name="COG4913"
                     /note="Uncharacterized conserved protein, contains a
                     C-terminal ATPase domain [Function unknown]"
                     /db_xref="CDD:443941"
     Region          1777..1829
                     /region_name="Rab_bind"
                     /note="Rab binding domain; pfam16704"
                     /db_xref="CDD:435531"
     Region          1831..1874
                     /region_name="GRIP"
                     /note="GRIP domain; pfam01465"
                     /db_xref="CDD:460221"
     CDS             1..1904
                     /gene="LOC144114391"
                     /coded_by="XM_077648097.1:143..5857"
                     /db_xref="GeneID:144114391"
ORIGIN      
        1 mdssqdqrte kgprvsvdda predlvkqvk rqlallqkak akcddlgrkc qekdkaidel
       61 qsqckrweqe haeleelrqa yhllqdsqek qyhsfevfee klaassedva rwrhkyqecl
      121 eklecteskm aaytqgtecl qqelqrleae nraatckvee ltqsqkqlqa eraalqqqlg
      181 sleqekrqea gseamleisq ghlkqkieel lsqckalqqe rdacsaaqea laqqaesase
      241 eaaslrqkia vlleerssle nqrdekmnec nllqeekhgl erqvsdlyqt keeleakeke
      301 fakilddnss lekllaeavs entqlqervn ylqekigeve aekqnlaael etatevssre
      361 slrfegvvke lenklawqkn vehelqvsrc qqveseklie nlkaalaaah qqqhtvefca
      421 etklqdlprt qtvpeeqlqv lkqekdglit vnqglemdak iaqaetafle eklaqmkkay
      481 ealidefqis vvkvllleke lsdtrsqkef imdelreved vqhfledegn ilrqeceeln
      541 artgrleadl endrihilkl ehereaaewr dqqsqrevek lraqlkeval dawkcqevfq
      601 yfpphfvepr llsmlkvlhr tcrhiletad qnlidvhvdd llpeagaalv pedfsddscw
      661 edrekevvrp vpgdiyedlk eklrsmpahf lasiksggil qhekqdveee vthegsrkdw
      721 gageerngel agfspqiill leerigglet qhegrvydlk sdleqkrrel memdeslriv
      781 lgklseaser ytklksehva lsqrlreand lsdataalle eelrillgky eqlvaqssrl
      841 eidlkeaccq krhfkfvdkt dgteltetkm ltlesrgvgt eemenlahqe qgqkecddsa
      901 vqeyaalqge veklrdenkh lseecsslaa aldsskitve elsgslkvlt eqkqlmeesh
      961 sqkvslleae vdvkaqlldq ihgklddqle qferlealln eknknlqdvr ndtvrkdsel
     1021 eslrvelshl rdqlvfyene carlrtafqp esgnenslqd ealcewynah rtmlrllsql
     1081 vmlasrqqhp hfsfdghgds dvlsearcly stlsaklesv cqseedppvt lscivtkaih
     1141 kdlvtelalm ksvetlldsl ssdvakatte kplneleeck dsakeiselq kllmekdell
     1201 kkfralilkl kkevadktgq idsmeeelss lkqeveknvq askqsvqnfq slqneydklq
     1261 dqyeairsnc kelegklssm ehdlavanre lsdagaekek lkeiteylnl eikelrqhne
     1321 ekeaklilme sqarlvkele ekllgrikvl eeqavesnkq lltekeqhas taaqlekvqk
     1381 eskmhtllnl eiadyertva elnnllkern eeldstrkes lsyqskianl vehigylegq
     1441 kkadderlla fketianlke glsksrkhee dllqtevrle sevkaaqlre qdlklgyael
     1501 lkkcqgless lrsmrenyqr avkalegtva slkeelssaq aelqackvef enykvrvhtv
     1561 lkqqkkstag aesgdsseaq eklqgvveqm rqkirdlteq lelsqsetea ardqcdrltq
     1621 rhqaitlele aretewkhrl eeaikqntmd msleldrqlq rqyeelsdky kkkltvqeaq
     1681 hcctlelqme kveelkreia elqqkllsrq gspqeetpai diatlerqeg egsesispep
     1741 pvltkplpws lvptggfkpl eqllredenl vaaedldvlh ekvresqkkv dhlaellnes
     1801 essnlrmteq nrvlkeeirr lernqerekh aqnleylkni imkfvtlrgg sekerlvpvl
     1861 ttmlklspee kkdleavvtg egqqdtaavs swggylprwt glvg
//
LOCUS       XP_077504224            1903 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114391 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504224
VERSION     XP_077504224.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648098.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1903
                     /product="uncharacterized protein LOC144114391 isoform X2"
                     /calculated_mol_wt=218096
     Region          23..>594
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          781..1590
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          1221..>1822
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          1830..1873
                     /region_name="GRIP"
                     /note="GRIP domain; pfam01465"
                     /db_xref="CDD:460221"
     CDS             1..1903
                     /gene="LOC144114391"
                     /coded_by="XM_077648098.1:143..5854"
                     /db_xref="GeneID:144114391"
ORIGIN      
        1 mdssqdqrte kgprvsvdda predlvkqvk rqlallqkak akcddlgrkc qekdkaidel
       61 qsqckrweqe haeleelrqa yhllqdsqek qyhsfevfee klaassedva rwrhkyqecl
      121 eklecteskm aaytqgtecl qqelqrleae nraatckvee ltqsqkqlqa eraalqqqlg
      181 sleqekrqea gseamleisq ghlkqkieel lsqckalqqe rdacsaaqea laqqaesase
      241 eaaslrqkia vlleerssle nqrdekmnec nllqeekhgl erqvsdlyqt keeleakeke
      301 fakilddnss lekllaeavs entqlqervn ylqekigeve aekqnlaael etatevssre
      361 slrfegvvke lenklawqkn vehelqvsrc qqveseklie nlkaalaaah qqqhtvefca
      421 etklqdlprt qtvpeeqlqv lkqekdglit vnqglemdak iaqaetafle eklaqmkkay
      481 ealidefqis vvkvllleke lsdtrsqkef imdelreved vqhfledegn ilrqeceeln
      541 artgrleadl endrihilkl ehereaaewr dqqsqrevek lraqlkeval dawkcqevfq
      601 yfpphfvepr llsmlkvlhr tcrhiletad qnlidvhvdd llpeagaalv pedfsddscw
      661 edrekevvrp vpgdiyedlk eklrsmpahf lasiksggil qhekqdveee vthegsrkdw
      721 gageerngel agfspqiill leerigglet qhegrvydlk sdleqkrrel memdeslriv
      781 lgklseaser ytklksehva lsqrlreand lsdataalle eelrillgky eqlvaqssrl
      841 eidlkeaccq krhfkfvdkt dgteltetkm ltlesrgvgt eemenlahqe qgqkecddsa
      901 vqeyaalqge veklrdenkh lseecsslaa aldsskitve elsgslkvlt eqkqlmeesh
      961 sqkvslleae vdvkaqlldq ihgklddqle qferlealln eknknlqdvr ndtvrkdsel
     1021 eslrvelshl rdqlvfyene carlrtafqp esgnenslqd ealcewynah rtmlrllsql
     1081 vmlasrqqhp hfsfdghgds dvlsearcly stlsaklesv cqseedppvt lscivtkaih
     1141 kdlvtelalm ksvetlldsl ssdvakatte kplneleeck dsakeiselq kllmekdell
     1201 kkfralilkl kkevadktgq idsmeeelss lkqeveknvq askqsvqnfq slqneydklq
     1261 dqyeairsnc kelegklssm ehdlavanre lsdagaekek lkeiteylnl eikelrqhne
     1321 ekeaklilme sqarlvkele ekllgrikvl eeqavesnkq lltekeqhas taaqlekvqk
     1381 eskmhtllnl eiadyertva elnnllkern eeldstrkes lsyqskianl vehigylegq
     1441 kkadderlla fketianlke glsksrkhee dllqtevrle sevkaaqlre qdlklgyael
     1501 lkkcqgless lrsmrenyqr avkalegtva slkeelssaq aelqackvef enykvrvhtv
     1561 lkqqkkstag aesgdsseaq eklqgvveqm rqkirdlteq lelsqsetea ardqcdrltq
     1621 rhqaitlele aretewkhrl eeaikqntmd msleldrqlq rqyeelsdky kkltvqeaqh
     1681 cctlelqmek veelkreiae lqqkllsrqg spqeetpaid iatlerqege gsesispepp
     1741 vltkplpwsl vptggfkple qllredenlv aaedldvlhe kvresqkkvd hlaellnese
     1801 ssnlrmteqn rvlkeeirrl ernqerekha qnleylknii mkfvtlrggs ekerlvpvlt
     1861 tmlklspeek kdleavvtge gqqdtaavss wggylprwtg lvg
//
LOCUS       XP_077504225            1903 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114391 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504225
VERSION     XP_077504225.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648099.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1903
                     /product="uncharacterized protein LOC144114391 isoform X3"
                     /calculated_mol_wt=218125
     Region          23..>594
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          781..1590
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <1519..>1706
                     /region_name="COG4913"
                     /note="Uncharacterized conserved protein, contains a
                     C-terminal ATPase domain [Function unknown]"
                     /db_xref="CDD:443941"
     Region          1776..1828
                     /region_name="Rab_bind"
                     /note="Rab binding domain; pfam16704"
                     /db_xref="CDD:435531"
     Region          1830..1873
                     /region_name="GRIP"
                     /note="GRIP domain; pfam01465"
                     /db_xref="CDD:460221"
     CDS             1..1903
                     /gene="LOC144114391"
                     /coded_by="XM_077648099.1:143..5854"
                     /db_xref="GeneID:144114391"
ORIGIN      
        1 mdssqdqrte kgprvsvdda predlvkqvk rqlallqkak akcddlgrkc qekdkaidel
       61 qsqckrweqe haeleelrqa yhllqdsqek qyhsfevfee klaassedva rwrhkyqecl
      121 eklecteskm aaytqgtecl qqelqrleae nraatckvee ltqsqkqlqa eraalqqqlg
      181 sleqekrqea gseamleisq ghlkqkieel lsqckalqqe rdacsaaqea laqqaesase
      241 eaaslrqkia vlleerssle nqrdekmnec nllqeekhgl erqvsdlyqt keeleakeke
      301 fakilddnss lekllaeavs entqlqervn ylqekigeve aekqnlaael etatevssre
      361 slrfegvvke lenklawqkn vehelqvsrc qqveseklie nlkaalaaah qqqhtvefca
      421 etklqdlprt qtvpeeqlqv lkqekdglit vnqglemdak iaqaetafle eklaqmkkay
      481 ealidefqis vvkvllleke lsdtrsqkef imdelreved vqhfledegn ilrqeceeln
      541 artgrleadl endrihilkl ehereaaewr dqqsqrevek lraqlkeval dawkcqevfq
      601 yfpphfvepr llsmlkvlhr tcrhiletad qnlidvhvdd llpeagaalv pedfsddscw
      661 edrekevvrp vpgdiyedlk eklrsmpahf lasiksggil qhekqdveee vthegsrkdw
      721 gageerngel agfspqiill leerigglet qhegrvydlk sdleqkrrel memdeslriv
      781 lgklseaser ytklksehva lsqrlreand lsdataalle eelrillgky eqlvaqssrl
      841 eidlkeaccq krhfkfvdkt dgteltetkm ltlesrgvgt eemenlahqe qgqkecddsa
      901 vqeyaalqge veklrdenkh lseecsslaa aldsskitve elsgslkvlt eqkqlmeesh
      961 sqkvslleae vdvkaqlldq ihgklddqle qferlealln eknknlqdvr ndtvrkdsel
     1021 eslrvelshl rdqlvfyene carlrtafqp esgnenslqd ealcewynah rtmlrllsql
     1081 vmlasrqqhp hfsfdghgds dvlsearcly stlsaklesv cqseedppvt lscivtkaih
     1141 kdlvtelalm ksvetlldsl ssdvakatte kplneleeck dsakeiselq kllmekdell
     1201 kkfralilkl kkevadktgq idsmeeelss lkqeveknvq askqsvqnfq slqneydklq
     1261 dqyeairsnc kelegklssm ehdlavanre lsdagaekek lkeiteylnl eikelrqhne
     1321 ekeaklilme sqarlvkele ekllgrikvl eeqavesnkq lltekeqhas taaqlekvqk
     1381 eskmhtllnl eiadyertva elnnllkern eeldstrkes lsyqskianl vehigylegq
     1441 kkadderlla fketianlke glsksrkhee dllqtevrle sevkaaqlre qdlklgyael
     1501 lkkcqgless lrsmrenyqr avkalegtva slkeelssaq aelqackvef enykvrvhtv
     1561 lkqqkkstag aesgdsseaq eklqgvveqm rqkirdlteq lelsqsetea ardqcdrltq
     1621 rhqaitlele aretewkhrl eeaikqntmd msleldrqlq rqyeelsdky kkkltvqeaq
     1681 hcctlelqme kveelkreia elqqkllsrq gspqeetpai diatlerqeg egsesispep
     1741 pvltkplpws lvptggfkpl eqllredenl aaedldvlhe kvresqkkvd hlaellnese
     1801 ssnlrmteqn rvlkeeirrl ernqerekha qnleylknii mkfvtlrggs ekerlvpvlt
     1861 tmlklspeek kdleavvtge gqqdtaavss wggylprwtg lvg
//
LOCUS       XP_077504226            1902 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114391 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077504226
VERSION     XP_077504226.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648100.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1902
                     /product="uncharacterized protein LOC144114391 isoform X4"
                     /calculated_mol_wt=217997
     Region          23..>594
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          781..1590
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          1221..>1821
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          1829..1872
                     /region_name="GRIP"
                     /note="GRIP domain; pfam01465"
                     /db_xref="CDD:460221"
     CDS             1..1902
                     /gene="LOC144114391"
                     /coded_by="XM_077648100.1:143..5851"
                     /db_xref="GeneID:144114391"
ORIGIN      
        1 mdssqdqrte kgprvsvdda predlvkqvk rqlallqkak akcddlgrkc qekdkaidel
       61 qsqckrweqe haeleelrqa yhllqdsqek qyhsfevfee klaassedva rwrhkyqecl
      121 eklecteskm aaytqgtecl qqelqrleae nraatckvee ltqsqkqlqa eraalqqqlg
      181 sleqekrqea gseamleisq ghlkqkieel lsqckalqqe rdacsaaqea laqqaesase
      241 eaaslrqkia vlleerssle nqrdekmnec nllqeekhgl erqvsdlyqt keeleakeke
      301 fakilddnss lekllaeavs entqlqervn ylqekigeve aekqnlaael etatevssre
      361 slrfegvvke lenklawqkn vehelqvsrc qqveseklie nlkaalaaah qqqhtvefca
      421 etklqdlprt qtvpeeqlqv lkqekdglit vnqglemdak iaqaetafle eklaqmkkay
      481 ealidefqis vvkvllleke lsdtrsqkef imdelreved vqhfledegn ilrqeceeln
      541 artgrleadl endrihilkl ehereaaewr dqqsqrevek lraqlkeval dawkcqevfq
      601 yfpphfvepr llsmlkvlhr tcrhiletad qnlidvhvdd llpeagaalv pedfsddscw
      661 edrekevvrp vpgdiyedlk eklrsmpahf lasiksggil qhekqdveee vthegsrkdw
      721 gageerngel agfspqiill leerigglet qhegrvydlk sdleqkrrel memdeslriv
      781 lgklseaser ytklksehva lsqrlreand lsdataalle eelrillgky eqlvaqssrl
      841 eidlkeaccq krhfkfvdkt dgteltetkm ltlesrgvgt eemenlahqe qgqkecddsa
      901 vqeyaalqge veklrdenkh lseecsslaa aldsskitve elsgslkvlt eqkqlmeesh
      961 sqkvslleae vdvkaqlldq ihgklddqle qferlealln eknknlqdvr ndtvrkdsel
     1021 eslrvelshl rdqlvfyene carlrtafqp esgnenslqd ealcewynah rtmlrllsql
     1081 vmlasrqqhp hfsfdghgds dvlsearcly stlsaklesv cqseedppvt lscivtkaih
     1141 kdlvtelalm ksvetlldsl ssdvakatte kplneleeck dsakeiselq kllmekdell
     1201 kkfralilkl kkevadktgq idsmeeelss lkqeveknvq askqsvqnfq slqneydklq
     1261 dqyeairsnc kelegklssm ehdlavanre lsdagaekek lkeiteylnl eikelrqhne
     1321 ekeaklilme sqarlvkele ekllgrikvl eeqavesnkq lltekeqhas taaqlekvqk
     1381 eskmhtllnl eiadyertva elnnllkern eeldstrkes lsyqskianl vehigylegq
     1441 kkadderlla fketianlke glsksrkhee dllqtevrle sevkaaqlre qdlklgyael
     1501 lkkcqgless lrsmrenyqr avkalegtva slkeelssaq aelqackvef enykvrvhtv
     1561 lkqqkkstag aesgdsseaq eklqgvveqm rqkirdlteq lelsqsetea ardqcdrltq
     1621 rhqaitlele aretewkhrl eeaikqntmd msleldrqlq rqyeelsdky kkltvqeaqh
     1681 cctlelqmek veelkreiae lqqkllsrqg spqeetpaid iatlerqege gsesispepp
     1741 vltkplpwsl vptggfkple qllredenla aedldvlhek vresqkkvdh laellneses
     1801 snlrmteqnr vlkeeirrle rnqerekhaq nleylkniim kfvtlrggse kerlvpvltt
     1861 mlklspeekk dleavvtgeg qqdtaavssw ggylprwtgl vg
//
LOCUS       XP_077504228            1886 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114391 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077504228
VERSION     XP_077504228.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648102.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1886
                     /product="uncharacterized protein LOC144114391 isoform X5"
                     /calculated_mol_wt=216130
     Region          23..>594
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          781..1572
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <1501..>1688
                     /region_name="COG4913"
                     /note="Uncharacterized conserved protein, contains a
                     C-terminal ATPase domain [Function unknown]"
                     /db_xref="CDD:443941"
     Region          1759..1811
                     /region_name="Rab_bind"
                     /note="Rab binding domain; pfam16704"
                     /db_xref="CDD:435531"
     Region          1813..1856
                     /region_name="GRIP"
                     /note="GRIP domain; pfam01465"
                     /db_xref="CDD:460221"
     CDS             1..1886
                     /gene="LOC144114391"
                     /coded_by="XM_077648102.1:143..5803"
                     /db_xref="GeneID:144114391"
ORIGIN      
        1 mdssqdqrte kgprvsvdda predlvkqvk rqlallqkak akcddlgrkc qekdkaidel
       61 qsqckrweqe haeleelrqa yhllqdsqek qyhsfevfee klaassedva rwrhkyqecl
      121 eklecteskm aaytqgtecl qqelqrleae nraatckvee ltqsqkqlqa eraalqqqlg
      181 sleqekrqea gseamleisq ghlkqkieel lsqckalqqe rdacsaaqea laqqaesase
      241 eaaslrqkia vlleerssle nqrdekmnec nllqeekhgl erqvsdlyqt keeleakeke
      301 fakilddnss lekllaeavs entqlqervn ylqekigeve aekqnlaael etatevssre
      361 slrfegvvke lenklawqkn vehelqvsrc qqveseklie nlkaalaaah qqqhtvefca
      421 etklqdlprt qtvpeeqlqv lkqekdglit vnqglemdak iaqaetafle eklaqmkkay
      481 ealidefqis vvkvllleke lsdtrsqkef imdelreved vqhfledegn ilrqeceeln
      541 artgrleadl endrihilkl ehereaaewr dqqsqrevek lraqlkeval dawkcqevfq
      601 yfpphfvepr llsmlkvlhr tcrhiletad qnlidvhvdd llpeagaalv pedfsddscw
      661 edrekevvrp vpgdiyedlk eklrsmpahf lasiksggil qhekqdveee vthegsrkdw
      721 gageerngel agfspqiill leerigglet qhegrvydlk sdleqkrrel memdeslriv
      781 lgklseaser ytklksehva lsqrlreand lsdataalle eelrillgky eqlvaqssrl
      841 eidlkeaccq krhfkfvdkt dgteltetkm ltlesrgvgt eemenlahqe qgqkecddsa
      901 vqeyaalqge veklrdenkh lseecsslaa aldsskitve elsgslkvlt eqkqlmeesh
      961 sqkvslleae vdvkaqlldq ihgklddqle qferlealln eknknlqdvr ndtvrkdsel
     1021 eslrvelshl rdqlvfyene carlrtafqp esgnenslqd ealcewynah rtmlrllsql
     1081 vmlasrqqhp hfsfdghgds dvlsearcly stlsaklesv cqseedppvt lscivtkaih
     1141 kdlvtelalm ksvetlldsl ssdvakatte kplneleeck dsakeiselq kllmekdell
     1201 kkfralilkl kkevadktgq idsmeeelss lkqeveknvq askqsvqnfq slqneydklq
     1261 dqyeairsnc kelegklssm ehdlavanre lsdagaekek lkeiteylnl eikelrqhne
     1321 ekeaklilme sqarlvkele ekllgrikvl eeqavesnkq lltekeqhas taaqlekvqk
     1381 eskmhtllnl eiadyertva elnnllkern eeldstrkes lsyqskianl vehigylegq
     1441 kkadderlla fketianlke glsksrkhee dllqtelgya ellkkcqgle sslrsmreny
     1501 qravkalegt vaslkeelss aqaelqackv efenykvrvh tvlkqqkkst agaesgdsse
     1561 aqeklqgvve qmrqkirdlt eqlelsqset eaardqcdrl tqrhqaitle learetewkh
     1621 rleeaikqnt mdmsleldrq lqrqyeelsd kykkkltvqe aqhcctlelq mekveelkre
     1681 iaelqqklls rqgspqeetp aidiatlerq egegsesisp eppvltkplp wslvptggfk
     1741 pleqllrede nlvaaedldv lhekvresqk kvdhlaelln esessnlrmt eqnrvlkeei
     1801 rrlernqere khaqnleylk niimkfvtlr ggsekerlvp vlttmlklsp eekkdleavv
     1861 tgegqqdtaa vsswggylpr wtglvg
//
LOCUS       XP_077504229            1885 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114391 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077504229
VERSION     XP_077504229.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648103.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1885
                     /product="uncharacterized protein LOC144114391 isoform X6"
                     /calculated_mol_wt=216002
     Region          23..>594
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          781..1572
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          1459..>1696
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          1758..1810
                     /region_name="Rab_bind"
                     /note="Rab binding domain; pfam16704"
                     /db_xref="CDD:435531"
     Region          1812..1855
                     /region_name="GRIP"
                     /note="GRIP domain; pfam01465"
                     /db_xref="CDD:460221"
     CDS             1..1885
                     /gene="LOC144114391"
                     /coded_by="XM_077648103.1:143..5800"
                     /db_xref="GeneID:144114391"
ORIGIN      
        1 mdssqdqrte kgprvsvdda predlvkqvk rqlallqkak akcddlgrkc qekdkaidel
       61 qsqckrweqe haeleelrqa yhllqdsqek qyhsfevfee klaassedva rwrhkyqecl
      121 eklecteskm aaytqgtecl qqelqrleae nraatckvee ltqsqkqlqa eraalqqqlg
      181 sleqekrqea gseamleisq ghlkqkieel lsqckalqqe rdacsaaqea laqqaesase
      241 eaaslrqkia vlleerssle nqrdekmnec nllqeekhgl erqvsdlyqt keeleakeke
      301 fakilddnss lekllaeavs entqlqervn ylqekigeve aekqnlaael etatevssre
      361 slrfegvvke lenklawqkn vehelqvsrc qqveseklie nlkaalaaah qqqhtvefca
      421 etklqdlprt qtvpeeqlqv lkqekdglit vnqglemdak iaqaetafle eklaqmkkay
      481 ealidefqis vvkvllleke lsdtrsqkef imdelreved vqhfledegn ilrqeceeln
      541 artgrleadl endrihilkl ehereaaewr dqqsqrevek lraqlkeval dawkcqevfq
      601 yfpphfvepr llsmlkvlhr tcrhiletad qnlidvhvdd llpeagaalv pedfsddscw
      661 edrekevvrp vpgdiyedlk eklrsmpahf lasiksggil qhekqdveee vthegsrkdw
      721 gageerngel agfspqiill leerigglet qhegrvydlk sdleqkrrel memdeslriv
      781 lgklseaser ytklksehva lsqrlreand lsdataalle eelrillgky eqlvaqssrl
      841 eidlkeaccq krhfkfvdkt dgteltetkm ltlesrgvgt eemenlahqe qgqkecddsa
      901 vqeyaalqge veklrdenkh lseecsslaa aldsskitve elsgslkvlt eqkqlmeesh
      961 sqkvslleae vdvkaqlldq ihgklddqle qferlealln eknknlqdvr ndtvrkdsel
     1021 eslrvelshl rdqlvfyene carlrtafqp esgnenslqd ealcewynah rtmlrllsql
     1081 vmlasrqqhp hfsfdghgds dvlsearcly stlsaklesv cqseedppvt lscivtkaih
     1141 kdlvtelalm ksvetlldsl ssdvakatte kplneleeck dsakeiselq kllmekdell
     1201 kkfralilkl kkevadktgq idsmeeelss lkqeveknvq askqsvqnfq slqneydklq
     1261 dqyeairsnc kelegklssm ehdlavanre lsdagaekek lkeiteylnl eikelrqhne
     1321 ekeaklilme sqarlvkele ekllgrikvl eeqavesnkq lltekeqhas taaqlekvqk
     1381 eskmhtllnl eiadyertva elnnllkern eeldstrkes lsyqskianl vehigylegq
     1441 kkadderlla fketianlke glsksrkhee dllqtelgya ellkkcqgle sslrsmreny
     1501 qravkalegt vaslkeelss aqaelqackv efenykvrvh tvlkqqkkst agaesgdsse
     1561 aqeklqgvve qmrqkirdlt eqlelsqset eaardqcdrl tqrhqaitle learetewkh
     1621 rleeaikqnt mdmsleldrq lqrqyeelsd kykkltvqea qhcctlelqm ekveelkrei
     1681 aelqqkllsr qgspqeetpa idiatlerqe gegsesispe ppvltkplpw slvptggfkp
     1741 leqllreden lvaaedldvl hekvresqkk vdhlaellne sessnlrmte qnrvlkeeir
     1801 rlernqerek haqnleylkn iimkfvtlrg gsekerlvpv lttmlklspe ekkdleavvt
     1861 gegqqdtaav sswggylprw tglvg
//
LOCUS       XP_077504230            1837 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114391 isoform X7 [Amblyomma
            americanum].
ACCESSION   XP_077504230
VERSION     XP_077504230.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648104.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1837
                     /product="uncharacterized protein LOC144114391 isoform X7"
                     /calculated_mol_wt=210449
     Region          23..>594
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          781..1535
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <1452..>1639
                     /region_name="COG4913"
                     /note="Uncharacterized conserved protein, contains a
                     C-terminal ATPase domain [Function unknown]"
                     /db_xref="CDD:443941"
     Region          1710..1762
                     /region_name="Rab_bind"
                     /note="Rab binding domain; pfam16704"
                     /db_xref="CDD:435531"
     Region          1764..1807
                     /region_name="GRIP"
                     /note="GRIP domain; pfam01465"
                     /db_xref="CDD:460221"
     CDS             1..1837
                     /gene="LOC144114391"
                     /coded_by="XM_077648104.1:143..5656"
                     /db_xref="GeneID:144114391"
ORIGIN      
        1 mdssqdqrte kgprvsvdda predlvkqvk rqlallqkak akcddlgrkc qekdkaidel
       61 qsqckrweqe haeleelrqa yhllqdsqek qyhsfevfee klaassedva rwrhkyqecl
      121 eklecteskm aaytqgtecl qqelqrleae nraatckvee ltqsqkqlqa eraalqqqlg
      181 sleqekrqea gseamleisq ghlkqkieel lsqckalqqe rdacsaaqea laqqaesase
      241 eaaslrqkia vlleerssle nqrdekmnec nllqeekhgl erqvsdlyqt keeleakeke
      301 fakilddnss lekllaeavs entqlqervn ylqekigeve aekqnlaael etatevssre
      361 slrfegvvke lenklawqkn vehelqvsrc qqveseklie nlkaalaaah qqqhtvefca
      421 etklqdlprt qtvpeeqlqv lkqekdglit vnqglemdak iaqaetafle eklaqmkkay
      481 ealidefqis vvkvllleke lsdtrsqkef imdelreved vqhfledegn ilrqeceeln
      541 artgrleadl endrihilkl ehereaaewr dqqsqrevek lraqlkeval dawkcqevfq
      601 yfpphfvepr llsmlkvlhr tcrhiletad qnlidvhvdd llpeagaalv pedfsddscw
      661 edrekevvrp vpgdiyedlk eklrsmpahf lasiksggil qhekqdveee vthegsrkdw
      721 gageerngel agfspqiill leerigglet qhegrvydlk sdleqkrrel memdeslriv
      781 lgklseaser ytklksehva lsqrlreand lsdataalle eelrillgky eqlvaqssrl
      841 eidlkeaccq krhfkfvdkt dgteltetkm ltlesrgvgt eemenlahqe qgqkecddsa
      901 vqeyaalqge veklrdenkh lseecsslaa aldsskitve elsgslkvlt eqkqlmeesh
      961 sqkvslleae vdvkaqlldq ihgklddqle qferlealln eknknlqdvr ndtvrkdsel
     1021 eslrvelshl rdqlvfyene carlrtafqp esgnenslqd ealcewynah rtmlrllsql
     1081 vmlasrqqhp hfsfdghgds dvlsearcly stlsaklesv cqseedppvt lscivtkaih
     1141 kdlvtelalm ksvetlldsl ssdvakatte kplneleeck dsakeiselq kllmekdell
     1201 kkfralilkl kkevadktgq idsmeeelss lkqeveknvq askqsvqnfq slqneydklq
     1261 dqyeairsnc kelegklssm ehdlavanre lsdagaekek lkeiteylnl eikeskmhtl
     1321 lnleiadyer tvaelnnllk erneeldstr keslsyqski anlvehigyl egqkkadder
     1381 llafketian lkeglsksrk heedllqtev rlesevkaaq lreqdlklgy aellkkcqgl
     1441 esslrsmren yqravkaleg tvaslkeels saqaelqack vefenykvrv htvlkqqkks
     1501 tagaesgdss eaqeklqgvv eqmrqkirdl teqlelsqse teaardqcdr ltqrhqaitl
     1561 elearetewk hrleeaikqn tmdmsleldr qlqrqyeels dkykkkltvq eaqhcctlel
     1621 qmekveelkr eiaelqqkll srqgspqeet paidiatler qegegsesis peppvltkpl
     1681 pwslvptggf kpleqllred enlvaaedld vlhekvresq kkvdhlaell nesessnlrm
     1741 teqnrvlkee irrlernqer ekhaqnleyl kniimkfvtl rggsekerlv pvlttmlkls
     1801 peekkdleav vtgegqqdta avsswggylp rwtglvg
//
LOCUS       XP_077504231            1836 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114391 isoform X8 [Amblyomma
            americanum].
ACCESSION   XP_077504231
VERSION     XP_077504231.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648105.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1836
                     /product="uncharacterized protein LOC144114391 isoform X8"
                     /calculated_mol_wt=210321
     Region          23..>594
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          781..1535
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <1452..>1642
                     /region_name="COG4913"
                     /note="Uncharacterized conserved protein, contains a
                     C-terminal ATPase domain [Function unknown]"
                     /db_xref="CDD:443941"
     Region          1709..1761
                     /region_name="Rab_bind"
                     /note="Rab binding domain; pfam16704"
                     /db_xref="CDD:435531"
     Region          1763..1806
                     /region_name="GRIP"
                     /note="GRIP domain; pfam01465"
                     /db_xref="CDD:460221"
     CDS             1..1836
                     /gene="LOC144114391"
                     /coded_by="XM_077648105.1:143..5653"
                     /db_xref="GeneID:144114391"
ORIGIN      
        1 mdssqdqrte kgprvsvdda predlvkqvk rqlallqkak akcddlgrkc qekdkaidel
       61 qsqckrweqe haeleelrqa yhllqdsqek qyhsfevfee klaassedva rwrhkyqecl
      121 eklecteskm aaytqgtecl qqelqrleae nraatckvee ltqsqkqlqa eraalqqqlg
      181 sleqekrqea gseamleisq ghlkqkieel lsqckalqqe rdacsaaqea laqqaesase
      241 eaaslrqkia vlleerssle nqrdekmnec nllqeekhgl erqvsdlyqt keeleakeke
      301 fakilddnss lekllaeavs entqlqervn ylqekigeve aekqnlaael etatevssre
      361 slrfegvvke lenklawqkn vehelqvsrc qqveseklie nlkaalaaah qqqhtvefca
      421 etklqdlprt qtvpeeqlqv lkqekdglit vnqglemdak iaqaetafle eklaqmkkay
      481 ealidefqis vvkvllleke lsdtrsqkef imdelreved vqhfledegn ilrqeceeln
      541 artgrleadl endrihilkl ehereaaewr dqqsqrevek lraqlkeval dawkcqevfq
      601 yfpphfvepr llsmlkvlhr tcrhiletad qnlidvhvdd llpeagaalv pedfsddscw
      661 edrekevvrp vpgdiyedlk eklrsmpahf lasiksggil qhekqdveee vthegsrkdw
      721 gageerngel agfspqiill leerigglet qhegrvydlk sdleqkrrel memdeslriv
      781 lgklseaser ytklksehva lsqrlreand lsdataalle eelrillgky eqlvaqssrl
      841 eidlkeaccq krhfkfvdkt dgteltetkm ltlesrgvgt eemenlahqe qgqkecddsa
      901 vqeyaalqge veklrdenkh lseecsslaa aldsskitve elsgslkvlt eqkqlmeesh
      961 sqkvslleae vdvkaqlldq ihgklddqle qferlealln eknknlqdvr ndtvrkdsel
     1021 eslrvelshl rdqlvfyene carlrtafqp esgnenslqd ealcewynah rtmlrllsql
     1081 vmlasrqqhp hfsfdghgds dvlsearcly stlsaklesv cqseedppvt lscivtkaih
     1141 kdlvtelalm ksvetlldsl ssdvakatte kplneleeck dsakeiselq kllmekdell
     1201 kkfralilkl kkevadktgq idsmeeelss lkqeveknvq askqsvqnfq slqneydklq
     1261 dqyeairsnc kelegklssm ehdlavanre lsdagaekek lkeiteylnl eikeskmhtl
     1321 lnleiadyer tvaelnnllk erneeldstr keslsyqski anlvehigyl egqkkadder
     1381 llafketian lkeglsksrk heedllqtev rlesevkaaq lreqdlklgy aellkkcqgl
     1441 esslrsmren yqravkaleg tvaslkeels saqaelqack vefenykvrv htvlkqqkks
     1501 tagaesgdss eaqeklqgvv eqmrqkirdl teqlelsqse teaardqcdr ltqrhqaitl
     1561 elearetewk hrleeaikqn tmdmsleldr qlqrqyeels dkykkltvqe aqhcctlelq
     1621 mekveelkre iaelqqklls rqgspqeetp aidiatlerq egegsesisp eppvltkplp
     1681 wslvptggfk pleqllrede nlvaaedldv lhekvresqk kvdhlaelln esessnlrmt
     1741 eqnrvlkeei rrlernqere khaqnleylk niimkfvtlr ggsekerlvp vlttmlklsp
     1801 eekkdleavv tgegqqdtaa vsswggylpr wtglvg
//
LOCUS       XP_077504232             639 aa            linear   INV 25-NOV-2025
DEFINITION  CUB and sushi domain-containing protein 2-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077504232
VERSION     XP_077504232.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648106.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..639
                     /product="CUB and sushi domain-containing protein 2-like
                     isoform X1"
                     /calculated_mol_wt=70824
     Region          <74..229
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     Region          104..365
                     /region_name="PHA02639"
                     /note="EEV host range protein; Provisional"
                     /db_xref="CDD:165022"
     Region          405..455
                     /region_name="Sushi"
                     /note="Sushi repeat (SCR repeat); pfam00084"
                     /db_xref="CDD:459664"
     CDS             1..639
                     /gene="LOC144114392"
                     /coded_by="XM_077648106.1:51..1970"
                     /db_xref="GeneID:144114392"
ORIGIN      
        1 msrtllafas yscgalhgaw prksmmlplc lamavvlcar salcqknnct epaslfihyr
       61 lqpgaehqke fpvgttltyc cgqgytnend twnatclqke deslywvigq hdcrpvscgh
      121 pghvlngrls gsefvfprsv vyecnrgywl kgksilycql daqwnapkpe chivqcgtle
      181 nitngfvnls gttfgskaef scnhgfklvg nstrtcgadg rwtdepphce edesnrkrka
      241 cqlekrqsrf kayhkgipvt pegpfehgte lvfhcepvge vhfegprtar cidekwdpke
      301 pfcyplgpsd irvrfpsaty taprgivyve pyshvvvecw snsrvelslk sddqsnviss
      361 trigqnfmrm kfwlalgksa tvicrhvtpg hsrnitvrgq pqfwcpplss ksqglrmerr
      421 tarsyhftcm egyqivgepd lhcqkhgwsd pipkcepisg asningtshq teqteaqtah
      481 pagpsgatls tpertekycl aekfleiapp glvialnetq vpenstfwaf cddgftfvgn
      541 vtavtclhdg twslpphitc ieacgnfdag pggpalegrk esydlgdsit lsckngtelq
      601 prierllclg drwsenavpi cvpakvktng akneerkra
//
LOCUS       XP_077504233             595 aa            linear   INV 25-NOV-2025
DEFINITION  sushi, von Willebrand factor type A, EGF and pentraxin
            domain-containing protein 1-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504233
VERSION     XP_077504233.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648107.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..595
                     /product="sushi, von Willebrand factor type A, EGF and
                     pentraxin domain-containing protein 1-like isoform X2"
                     /calculated_mol_wt=65860
     Region          <74..229
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     Region          171..>303
                     /region_name="PHA02817"
                     /note="EEV Host range protein; Provisional"
                     /db_xref="CDD:165167"
     Region          361..411
                     /region_name="Sushi"
                     /note="Sushi repeat (SCR repeat); pfam00084"
                     /db_xref="CDD:459664"
     CDS             1..595
                     /gene="LOC144114392"
                     /coded_by="XM_077648107.1:51..1838"
                     /db_xref="GeneID:144114392"
ORIGIN      
        1 msrtllafas yscgalhgaw prksmmlplc lamavvlcar salcqknnct epaslfihyr
       61 lqpgaehqke fpvgttltyc cgqgytnend twnatclqke deslywvigq hdcrpvscgh
      121 pghvlngrls gsefvfprsv vyecnrgywl kgksilycql daqwnapkpe chivqcgtle
      181 nitngfvnls gttfgskaef scnhgfklvg nstrtcgadg rwtdepphce edesnrkrka
      241 cqlekrqsrf kayhkgipvt pegpfehgte lvfhcepvge vhfegprtar cidekwdpke
      301 pfcyplgpsd irvrfpsaty taprgivyve pyshvvvecw snsrvelslk sddqsnaqfw
      361 cpplssksqg lrmerrtars yhftcmegyq ivgepdlhcq khgwsdpipk cepisgasni
      421 ngtshqteqt eaqtahpagp sgatlstper tekyclaekf leiappglvi alnetqvpen
      481 stfwafcddg ftfvgnvtav tclhdgtwsl pphitcieac gnfdagpggp alegrkesyd
      541 lgdsitlsck ngtelqprie rllclgdrws enavpicvpa kvktngakne erkra
//
LOCUS       XP_077504234             581 aa            linear   INV 25-NOV-2025
DEFINITION  complement receptor type 1-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077504234
VERSION     XP_077504234.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648108.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..581
                     /product="complement receptor type 1-like isoform X3"
                     /calculated_mol_wt=64309
     Region          <17..>177
                     /region_name="PHA02831"
                     /note="EEV host range protein; Provisional"
                     /db_xref="CDD:165176"
     Region          113..>245
                     /region_name="PHA02817"
                     /note="EEV Host range protein; Provisional"
                     /db_xref="CDD:165167"
     Region          118..172
                     /region_name="CCP"
                     /note="Complement control protein (CCP) modules (aka short
                     consensus repeats SCRs or SUSHI repeats) have been
                     identified in several proteins of the complement system;
                     cd00033"
                     /db_xref="CDD:153056"
     Site            order(128,145)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     Region          347..397
                     /region_name="Sushi"
                     /note="Sushi repeat (SCR repeat); pfam00084"
                     /db_xref="CDD:459664"
     Region          <473..564
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     CDS             1..581
                     /gene="LOC144114392"
                     /coded_by="XM_077648108.1:51..1796"
                     /db_xref="GeneID:144114392"
ORIGIN      
        1 msrtllafas yscgalhgaw prksmmlplc lamavvlcar salcqknnct epaslfihyr
       61 lqpgaehqke fpvgttltyc cgqgytnend twnatclqke deslywvigq hdcrlvqcgt
      121 lenitngfvn lsgttfgska efscnhgfkl vgnstrtcga dgrwtdepph ceedesnrkr
      181 kacqlekrqs rfkayhkgip vtpegpfehg telvfhcepv gevhfegprt arcidekwdp
      241 kepfcyplgp sdirvrfpsa tytaprgivy vepyshvvve cwsnsrvels lksddqsnvi
      301 sstrigqnfm rmkfwlalgk satvicrhvt pghsrnitvr gqpqfwcppl ssksqglrme
      361 rrtarsyhft cmegyqivge pdlhcqkhgw sdpipkcepi sgasningts hqteqteaqt
      421 ahpagpsgat lstperteky claekfleia ppglvialne tqvpenstfw afcddgftfv
      481 gnvtavtclh dgtwslpphi tcieacgnfd agpggpaleg rkesydlgds itlsckngte
      541 lqprierllc lgdrwsenav picvpakvkt ngakneerkr a
//
LOCUS       XP_077504235             513 aa            linear   INV 25-NOV-2025
DEFINITION  sushi, von Willebrand factor type A, EGF and pentraxin
            domain-containing protein 1-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077504235
VERSION     XP_077504235.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648109.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..513
                     /product="sushi, von Willebrand factor type A, EGF and
                     pentraxin domain-containing protein 1-like isoform X4"
                     /calculated_mol_wt=56333
     Region          44..329
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     CDS             1..513
                     /gene="LOC144114392"
                     /coded_by="XM_077648109.1:49..1590"
                     /db_xref="GeneID:144114392"
ORIGIN      
        1 msrtllafas yscgalhgaw prksmmlplc lamavvlcar salcqknnct epaslfihyr
       61 lqpgaehqke fpvgttltyc cgqgytnend twnatclqke deslywvigq hdcrpvscgh
      121 pghvlngrls gsefvfprsv vyecnrgywl kgksilycql daqwnapkpe chivqcgtle
      181 nitngfvnls gttfgskaef scnhgfklvg nstrtcgadg rwtdepphce visstrigqn
      241 fmrmkfwlal gksatvicrh vtpghsrnit vrgqpqfwcp plssksqglr merrtarsyh
      301 ftcmegyqiv gepdlhcqkh gwsdpipkce pisgasning tshqteqtea qtahpagpsg
      361 atlstperte kyclaekfle iappglvial netqvpenst fwafcddgft fvgnvtavtc
      421 lhdgtwslpp hitcieacgn fdagpggpal egrkesydlg dsitlsckng telqprierl
      481 lclgdrwsen avpicvpakv ktngakneer kra
//
LOCUS       XP_077504236             500 aa            linear   INV 25-NOV-2025
DEFINITION  protein lev-9-like isoform X5 [Amblyomma americanum].
ACCESSION   XP_077504236
VERSION     XP_077504236.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648110.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..500
                     /product="protein lev-9-like isoform X5"
                     /calculated_mol_wt=55468
     Region          <74..229
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     Region          171..>303
                     /region_name="PHA02817"
                     /note="EEV Host range protein; Provisional"
                     /db_xref="CDD:165167"
     CDS             1..500
                     /gene="LOC144114392"
                     /coded_by="XM_077648110.1:50..1552"
                     /db_xref="GeneID:144114392"
ORIGIN      
        1 msrtllafas yscgalhgaw prksmmlplc lamavvlcar salcqknnct epaslfihyr
       61 lqpgaehqke fpvgttltyc cgqgytnend twnatclqke deslywvigq hdcrpvscgh
      121 pghvlngrls gsefvfprsv vyecnrgywl kgksilycql daqwnapkpe chivqcgtle
      181 nitngfvnls gttfgskaef scnhgfklvg nstrtcgadg rwtdepphce edesnrkrka
      241 cqlekrqsrf kayhkgipvt pegpfehgte lvfhcepvge vhfegprtar cidekwdpke
      301 pfcyplgpsd irvrfpsaty taprgivyve pyshvvvecw snsrvelslk sddqsnekyc
      361 laekfleiap pglvialnet qvpenstfwa fcddgftfvg nvtavtclhd gtwslpphit
      421 cieacgnfda gpggpalegr kesydlgdsi tlsckngtel qprierllcl gdrwsenavp
      481 icvpakvktn gakneerkra
//
LOCUS       XP_077504237             469 aa            linear   INV 25-NOV-2025
DEFINITION  C4b-binding protein alpha chain-like isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077504237
VERSION     XP_077504237.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648111.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..469
                     /product="C4b-binding protein alpha chain-like isoform X6"
                     /calculated_mol_wt=51369
     Region          44..285
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     CDS             1..469
                     /gene="LOC144114392"
                     /coded_by="XM_077648111.1:48..1457"
                     /db_xref="GeneID:144114392"
ORIGIN      
        1 msrtllafas yscgalhgaw prksmmlplc lamavvlcar salcqknnct epaslfihyr
       61 lqpgaehqke fpvgttltyc cgqgytnend twnatclqke deslywvigq hdcrpvscgh
      121 pghvlngrls gsefvfprsv vyecnrgywl kgksilycql daqwnapkpe chivqcgtle
      181 nitngfvnls gttfgskaef scnhgfklvg nstrtcgadg rwtdepphce aqfwcpplss
      241 ksqglrmerr tarsyhftcm egyqivgepd lhcqkhgwsd pipkcepisg asningtshq
      301 teqteaqtah pagpsgatls tpertekycl aekfleiapp glvialnetq vpenstfwaf
      361 cddgftfvgn vtavtclhdg twslpphitc ieacgnfdag pggpalegrk esydlgdsit
      421 lsckngtelq prierllclg drwsenavpi cvpakvktng akneerkra
//
LOCUS       XP_077504238             436 aa            linear   INV 25-NOV-2025
DEFINITION  protein lev-9-like isoform X7 [Amblyomma americanum].
ACCESSION   XP_077504238
VERSION     XP_077504238.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648112.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..436
                     /product="protein lev-9-like isoform X7"
                     /calculated_mol_wt=48384
     Region          <74..229
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     Region          171..>303
                     /region_name="PHA02817"
                     /note="EEV Host range protein; Provisional"
                     /db_xref="CDD:165167"
     CDS             1..436
                     /gene="LOC144114392"
                     /coded_by="XM_077648112.1:50..1360"
                     /db_xref="GeneID:144114392"
ORIGIN      
        1 msrtllafas yscgalhgaw prksmmlplc lamavvlcar salcqknnct epaslfihyr
       61 lqpgaehqke fpvgttltyc cgqgytnend twnatclqke deslywvigq hdcrpvscgh
      121 pghvlngrls gsefvfprsv vyecnrgywl kgksilycql daqwnapkpe chivqcgtle
      181 nitngfvnls gttfgskaef scnhgfklvg nstrtcgadg rwtdepphce edesnrkrka
      241 cqlekrqsrf kayhkgipvt pegpfehgte lvfhcepvge vhfegprtar cidekwdpke
      301 pfcyplgpsd irvrfpsaty taprgivyve pyshvvvecw snsrvelslk sddqsngiea
      361 cgnfdagpgg palegrkesy dlgdsitlsc kngtelqpri erllclgdrw senavpicvp
      421 akvktngakn eerkra
//
LOCUS       XP_077504239             374 aa            linear   INV 25-NOV-2025
DEFINITION  protein lev-9-like isoform X8 [Amblyomma americanum].
ACCESSION   XP_077504239
VERSION     XP_077504239.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648113.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..374
                     /product="protein lev-9-like isoform X8"
                     /calculated_mol_wt=40978
     Region          44..>234
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     CDS             1..374
                     /gene="LOC144114392"
                     /coded_by="XM_077648113.1:46..1170"
                     /db_xref="GeneID:144114392"
ORIGIN      
        1 msrtllafas yscgalhgaw prksmmlplc lamavvlcar salcqknnct epaslfihyr
       61 lqpgaehqke fpvgttltyc cgqgytnend twnatclqke deslywvigq hdcrpvscgh
      121 pghvlngrls gsefvfprsv vyecnrgywl kgksilycql daqwnapkpe chivqcgtle
      181 nitngfvnls gttfgskaef scnhgfklvg nstrtcgadg rwtdepphce ekyclaekfl
      241 eiappglvia lnetqvpens tfwafcddgf tfvgnvtavt clhdgtwslp phitcieacg
      301 nfdagpggpa legrkesydl gdsitlsckn gtelqprier llclgdrwse navpicvpak
      361 vktngaknee rkra
//
LOCUS       XP_077504240            1268 aa            linear   INV 25-NOV-2025
DEFINITION  sushi, von Willebrand factor type A, EGF and pentraxin
            domain-containing protein 1-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504240
VERSION     XP_077504240.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648114.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1268
                     /product="sushi, von Willebrand factor type A, EGF and
                     pentraxin domain-containing protein 1-like isoform X1"
                     /calculated_mol_wt=139609
     Region          6..274
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     Region          <520..>620
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     Region          648..695
                     /region_name="CCP"
                     /note="Domain abundant in complement control proteins;
                     SUSHI repeat; short complement-like repeat (SCR);
                     smart00032"
                     /db_xref="CDD:214478"
     Region          <817..848
                     /region_name="Sushi"
                     /note="Sushi repeat (SCR repeat); pfam00084"
                     /db_xref="CDD:459664"
     Region          <844..1039
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          1138..1189
                     /region_name="Sushi"
                     /note="Sushi repeat (SCR repeat); pfam00084"
                     /db_xref="CDD:459664"
     CDS             1..1268
                     /gene="LOC144114394"
                     /coded_by="XM_077648114.1:140..3946"
                     /db_xref="GeneID:144114394"
ORIGIN      
        1 mrpplflama vlacaksalc qeakcpepkn myvhyrvqgg eefqeefpvg ttltyrcgpg
       61 yipreiktwn atcyqnydgs vdwnigkhdc rpvscgypgd vrygrlldav fvfprrvryq
      121 cnrgyrlegr eelycqsdaq wsapkpecri vecgplesig ngtvrlsgtv fgskaefscn
      181 hgfklvgnsa rtcgadgqws desphceevw cseemllefa qrngtvvvgd qpisgrqrsn
      241 trvthicppn tdlvgseint cqengawlfd ppackakciv qpgpgsqvqr rtsgwrtrfs
      301 vvesgtqvse fehnlfvecv ngyvlrsrrm evelplsckd gvwtpsgpwc iekpctihgp
      361 fpegveaeqy gevphgqevr frcmsgymmq nteneqhqpr clfgridgsy psckpddcri
      421 edlpfvengr lragqsvshg slaiyecnps lelqqrgggv rchfgqwigt splcvpkkci
      481 apeltgtilq rkksiakggd mrersltywt tevlpgaevt dgdqnlfvkc rqgyhllntr
      541 epetqvlcks gawqppppwc aedgcriadl ppvkngrplg dgivapiflg tyacdpafel
      601 haagrirchq gqwlgsppsc vskdesnrkr kvcklenkhs rfkayhkgip vtpegpfehg
      661 telvfhcepv gevrfegprt arcidgkwdp qvplcyplkp sdiavrflna iytaprgivy
      721 vdpnsyvvvd cwsnssvels lksddqsnvi sstrirqnfm rmkfwlalgk sasvicrrqv
      781 tpdnprsiiv rgqpqfwcpp lssnsqglrm krrtersyyf tcmegyqivg epdlhcqkhg
      841 wsdpipkcep isgasninga shqteqtvaq tahpagpsga tlstpertds qsterapaet
      901 phstakvaad pspphvvnen eapktegehg nratdspvln prystqeqig tqsgvskdln
      961 agsnngstme pkpipgqrar appqdvnqts hskrmnsqtc rqnttsdsct dlgnrngppv
     1021 escegcdeep piaesitann asatrkrdke strdasgsra tdhehstggp wqdanttapp
     1081 aqvpgklsnr qepheeqyls tsttttvkpt msssteatmp lssnekyclg kkffeiappg
     1141 lvialnetqv penstfwafc ddgftlvgnv tavsclhdgt wslppnitci eacgnfdagp
     1201 ggpalegrke sydlgdsitl sckngtelqp rierllclgd rwsenavpic vpakvktnga
     1261 kneerkra
//
LOCUS       XP_077504241            1260 aa            linear   INV 25-NOV-2025
DEFINITION  sushi, von Willebrand factor type A, EGF and pentraxin
            domain-containing protein 1-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504241
VERSION     XP_077504241.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648115.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1260
                     /product="sushi, von Willebrand factor type A, EGF and
                     pentraxin domain-containing protein 1-like isoform X2"
                     /calculated_mol_wt=138720
     Region          6..274
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     Region          <512..>612
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     Region          640..687
                     /region_name="CCP"
                     /note="Domain abundant in complement control proteins;
                     SUSHI repeat; short complement-like repeat (SCR);
                     smart00032"
                     /db_xref="CDD:214478"
     Region          <809..840
                     /region_name="Sushi"
                     /note="Sushi repeat (SCR repeat); pfam00084"
                     /db_xref="CDD:459664"
     Region          <836..1031
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          1130..1181
                     /region_name="Sushi"
                     /note="Sushi repeat (SCR repeat); pfam00084"
                     /db_xref="CDD:459664"
     CDS             1..1260
                     /gene="LOC144114394"
                     /coded_by="XM_077648115.1:140..3922"
                     /db_xref="GeneID:144114394"
ORIGIN      
        1 mrpplflama vlacaksalc qeakcpepkn myvhyrvqgg eefqeefpvg ttltyrcgpg
       61 yipreiktwn atcyqnydgs vdwnigkhdc rpvscgypgd vrygrlldav fvfprrvryq
      121 cnrgyrlegr eelycqsdaq wsapkpecri vecgplesig ngtvrlsgtv fgskaefscn
      181 hgfklvgnsa rtcgadgqws desphceevw cseemllefa qrngtvvvgd qpisgrqrsn
      241 trvthicppn tdlvgseint cqengawlfd ppackakciv qpgpgsqvqr rtsgwrtrfs
      301 vvesgtqvse fehnlfvecv ngyvlrsrrm evelplsckd gvwtpsgpwc iekpctihgp
      361 fpegveaeqy gevphgqevr frcmsgymmq nteneqhqpr clfgridgsy psckpddcri
      421 edlpfvengr lragqsvshg slaiyecnps lelqqrgggv rchfgqwigt splcvpkkci
      481 apeltgtilq rkksiaklty wttevlpgae vtdgdqnlfv kcrqgyhlln trepetqvlc
      541 ksgawqpppp wcaedgcria dlppvkngrp lgdgivapif lgtyacdpaf elhaagrirc
      601 hqgqwlgspp scvskdesnr krkvcklenk hsrfkayhkg ipvtpegpfe hgtelvfhce
      661 pvgevrfegp rtarcidgkw dpqvplcypl kpsdiavrfl naiytaprgi vyvdpnsyvv
      721 vdcwsnssve lslksddqsn visstrirqn fmrmkfwlal gksasvicrr qvtpdnprsi
      781 ivrgqpqfwc pplssnsqgl rmkrrtersy yftcmegyqi vgepdlhcqk hgwsdpipkc
      841 episgasnin gashqteqtv aqtahpagps gatlstpert dsqsterapa etphstakva
      901 adpspphvvn eneapktege hgnratdspv lnprystqeq igtqsgvskd lnagsnngst
      961 mepkpipgqr arappqdvnq tshskrmnsq tcrqnttsds ctdlgnrngp pvescegcde
     1021 eppiaesita nnasatrkrd kestrdasgs ratdhehstg gpwqdantta ppaqvpgkls
     1081 nrqepheeqy lststtttvk ptmsssteat mplssnekyc lgkkffeiap pglvialnet
     1141 qvpenstfwa fcddgftlvg nvtavsclhd gtwslppnit cieacgnfda gpggpalegr
     1201 kesydlgdsi tlsckngtel qprierllcl gdrwsenavp icvpakvktn gakneerkra
//
LOCUS       XP_077504243            1208 aa            linear   INV 25-NOV-2025
DEFINITION  sushi, von Willebrand factor type A, EGF and pentraxin
            domain-containing protein 1-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077504243
VERSION     XP_077504243.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648117.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1208
                     /product="sushi, von Willebrand factor type A, EGF and
                     pentraxin domain-containing protein 1-like isoform X3"
                     /calculated_mol_wt=133326
     Region          6..274
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     Region          588..635
                     /region_name="CCP"
                     /note="Domain abundant in complement control proteins;
                     SUSHI repeat; short complement-like repeat (SCR);
                     smart00032"
                     /db_xref="CDD:214478"
     Region          <757..788
                     /region_name="Sushi"
                     /note="Sushi repeat (SCR repeat); pfam00084"
                     /db_xref="CDD:459664"
     Region          <784..979
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          1078..1129
                     /region_name="Sushi"
                     /note="Sushi repeat (SCR repeat); pfam00084"
                     /db_xref="CDD:459664"
     CDS             1..1208
                     /gene="LOC144114394"
                     /coded_by="XM_077648117.1:140..3766"
                     /db_xref="GeneID:144114394"
ORIGIN      
        1 mrpplflama vlacaksalc qeakcpepkn myvhyrvqgg eefqeefpvg ttltyrcgpg
       61 yipreiktwn atcyqnydgs vdwnigkhdc rpvscgypgd vrygrlldav fvfprrvryq
      121 cnrgyrlegr eelycqsdaq wsapkpecri vecgplesig ngtvrlsgtv fgskaefscn
      181 hgfklvgnsa rtcgadgqws desphceevw cseemllefa qrngtvvvgd qpisgrqrsn
      241 trvthicppn tdlvgseint cqengawlfd ppackakciv qpgpgsqvqr rtsgwrtrfs
      301 vvesgtqvse fehnlfvecv ngyvlrsrrm evelplsckd gvwtpsgpwc iekpctihgp
      361 fpegveaeqy gevphgqevr frcmsgymmq nteneqhqpr clfgridgsy psckpddcri
      421 edlpfvengr lragqsvshg slaiyecnps lelqqrgggv rchfgqwigt splcvpkkci
      481 apeltgtilq rkksiakggd mrersltywt tevlpgaevt dgdqnlfvkc rqgyhllntr
      541 epetqvlcks gawqppppwc aeedesnrkr kvcklenkhs rfkayhkgip vtpegpfehg
      601 telvfhcepv gevrfegprt arcidgkwdp qvplcyplkp sdiavrflna iytaprgivy
      661 vdpnsyvvvd cwsnssvels lksddqsnvi sstrirqnfm rmkfwlalgk sasvicrrqv
      721 tpdnprsiiv rgqpqfwcpp lssnsqglrm krrtersyyf tcmegyqivg epdlhcqkhg
      781 wsdpipkcep isgasninga shqteqtvaq tahpagpsga tlstpertds qsterapaet
      841 phstakvaad pspphvvnen eapktegehg nratdspvln prystqeqig tqsgvskdln
      901 agsnngstme pkpipgqrar appqdvnqts hskrmnsqtc rqnttsdsct dlgnrngppv
      961 escegcdeep piaesitann asatrkrdke strdasgsra tdhehstggp wqdanttapp
     1021 aqvpgklsnr qepheeqyls tsttttvkpt msssteatmp lssnekyclg kkffeiappg
     1081 lvialnetqv penstfwafc ddgftlvgnv tavsclhdgt wslppnitci eacgnfdagp
     1141 ggpalegrke sydlgdsitl sckngtelqp rierllclgd rwsenavpic vpakvktnga
     1201 kneerkra
//
LOCUS       XP_077504244            1207 aa            linear   INV 25-NOV-2025
DEFINITION  sushi, von Willebrand factor type A, EGF and pentraxin
            domain-containing protein 1-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077504244
VERSION     XP_077504244.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648118.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1207
                     /product="sushi, von Willebrand factor type A, EGF and
                     pentraxin domain-containing protein 1-like isoform X4"
                     /calculated_mol_wt=132958
     Region          6..274
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     Region          <459..>559
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     Region          587..634
                     /region_name="CCP"
                     /note="Domain abundant in complement control proteins;
                     SUSHI repeat; short complement-like repeat (SCR);
                     smart00032"
                     /db_xref="CDD:214478"
     Region          <756..787
                     /region_name="Sushi"
                     /note="Sushi repeat (SCR repeat); pfam00084"
                     /db_xref="CDD:459664"
     Region          <783..978
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          1077..1128
                     /region_name="Sushi"
                     /note="Sushi repeat (SCR repeat); pfam00084"
                     /db_xref="CDD:459664"
     CDS             1..1207
                     /gene="LOC144114394"
                     /coded_by="XM_077648118.1:140..3763"
                     /db_xref="GeneID:144114394"
ORIGIN      
        1 mrpplflama vlacaksalc qeakcpepkn myvhyrvqgg eefqeefpvg ttltyrcgpg
       61 yipreiktwn atcyqnydgs vdwnigkhdc rpvscgypgd vrygrlldav fvfprrvryq
      121 cnrgyrlegr eelycqsdaq wsapkpecri vecgplesig ngtvrlsgtv fgskaefscn
      181 hgfklvgnsa rtcgadgqws desphceevw cseemllefa qrngtvvvgd qpisgrqrsn
      241 trvthicppn tdlvgseint cqengawlfd ppackakciv qpgpgsqvqr rtsgwrtrfs
      301 vvesgtqvse fehnlfvecv ngyvlrsrrm evelplsckd gvwtpsgpwc iekpctihgp
      361 fpegveaeqy gevphgqevr frcmsgymmq nteneqhqpr clfgridgsy psckpekcia
      421 peltgtilqr kksiakggdm rersltywtt evlpgaevtd gdqnlfvkcr qgyhllntre
      481 petqvlcksg awqppppwca edgcriadlp pvkngrplgd givapiflgt yacdpafelh
      541 aagrirchqg qwlgsppscv skdesnrkrk vcklenkhsr fkayhkgipv tpegpfehgt
      601 elvfhcepvg evrfegprta rcidgkwdpq vplcyplkps diavrflnai ytaprgivyv
      661 dpnsyvvvdc wsnssvelsl ksddqsnvis strirqnfmr mkfwlalgks asvicrrqvt
      721 pdnprsiivr gqpqfwcppl ssnsqglrmk rrtersyyft cmegyqivge pdlhcqkhgw
      781 sdpipkcepi sgasningas hqteqtvaqt ahpagpsgat lstpertdsq sterapaetp
      841 hstakvaadp spphvvnene apktegehgn ratdspvlnp rystqeqigt qsgvskdlna
      901 gsnngstmep kpipgqrara ppqdvnqtsh skrmnsqtcr qnttsdsctd lgnrngppve
      961 scegcdeepp iaesitanna satrkrdkes trdasgsrat dhehstggpw qdanttappa
     1021 qvpgklsnrq epheeqylst sttttvkptm sssteatmpl ssnekyclgk kffeiappgl
     1081 vialnetqvp enstfwafcd dgftlvgnvt avsclhdgtw slppnitcie acgnfdagpg
     1141 gpalegrkes ydlgdsitls ckngtelqpr ierllclgdr wsenavpicv pakvktngak
     1201 neerkra
//
LOCUS       XP_077504245            1204 aa            linear   INV 25-NOV-2025
DEFINITION  sushi, von Willebrand factor type A, EGF and pentraxin
            domain-containing protein 1-like isoform X5 [Amblyomma americanum].
ACCESSION   XP_077504245
VERSION     XP_077504245.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648119.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1204
                     /product="sushi, von Willebrand factor type A, EGF and
                     pentraxin domain-containing protein 1-like isoform X5"
                     /calculated_mol_wt=132577
     Region          6..274
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     Region          <520..>620
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     Region          648..695
                     /region_name="CCP"
                     /note="Domain abundant in complement control proteins;
                     SUSHI repeat; short complement-like repeat (SCR);
                     smart00032"
                     /db_xref="CDD:214478"
     Region          <817..848
                     /region_name="Sushi"
                     /note="Sushi repeat (SCR repeat); pfam00084"
                     /db_xref="CDD:459664"
     Region          <844..1039
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     CDS             1..1204
                     /gene="LOC144114394"
                     /coded_by="XM_077648119.1:140..3754"
                     /db_xref="GeneID:144114394"
ORIGIN      
        1 mrpplflama vlacaksalc qeakcpepkn myvhyrvqgg eefqeefpvg ttltyrcgpg
       61 yipreiktwn atcyqnydgs vdwnigkhdc rpvscgypgd vrygrlldav fvfprrvryq
      121 cnrgyrlegr eelycqsdaq wsapkpecri vecgplesig ngtvrlsgtv fgskaefscn
      181 hgfklvgnsa rtcgadgqws desphceevw cseemllefa qrngtvvvgd qpisgrqrsn
      241 trvthicppn tdlvgseint cqengawlfd ppackakciv qpgpgsqvqr rtsgwrtrfs
      301 vvesgtqvse fehnlfvecv ngyvlrsrrm evelplsckd gvwtpsgpwc iekpctihgp
      361 fpegveaeqy gevphgqevr frcmsgymmq nteneqhqpr clfgridgsy psckpddcri
      421 edlpfvengr lragqsvshg slaiyecnps lelqqrgggv rchfgqwigt splcvpkkci
      481 apeltgtilq rkksiakggd mrersltywt tevlpgaevt dgdqnlfvkc rqgyhllntr
      541 epetqvlcks gawqppppwc aedgcriadl ppvkngrplg dgivapiflg tyacdpafel
      601 haagrirchq gqwlgsppsc vskdesnrkr kvcklenkhs rfkayhkgip vtpegpfehg
      661 telvfhcepv gevrfegprt arcidgkwdp qvplcyplkp sdiavrflna iytaprgivy
      721 vdpnsyvvvd cwsnssvels lksddqsnvi sstrirqnfm rmkfwlalgk sasvicrrqv
      781 tpdnprsiiv rgqpqfwcpp lssnsqglrm krrtersyyf tcmegyqivg epdlhcqkhg
      841 wsdpipkcep isgasninga shqteqtvaq tahpagpsga tlstpertds qsterapaet
      901 phstakvaad pspphvvnen eapktegehg nratdspvln prystqeqig tqsgvskdln
      961 agsnngstme pkpipgqrar appqdvnqts hskrmnsqtc rqnttsdsct dlgnrngppv
     1021 escegcdeep piaesitann asatrkrdke strdasgsra tdhehstggp wqdanttapp
     1081 aqvpgklsnr qepheeqyls tsttttvkpt msssteatmp lssngieacg nfdagpggpa
     1141 legrkesydl gdsitlsckn gtelqprier llclgdrwse navpicvpak vktngaknee
     1201 rkra
//
LOCUS       XP_077504246            1199 aa            linear   INV 25-NOV-2025
DEFINITION  sushi, von Willebrand factor type A, EGF and pentraxin
            domain-containing protein 1-like isoform X6 [Amblyomma americanum].
ACCESSION   XP_077504246
VERSION     XP_077504246.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648120.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1199
                     /product="sushi, von Willebrand factor type A, EGF and
                     pentraxin domain-containing protein 1-like isoform X6"
                     /calculated_mol_wt=132069
     Region          6..274
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     Region          <451..>551
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     Region          579..626
                     /region_name="CCP"
                     /note="Domain abundant in complement control proteins;
                     SUSHI repeat; short complement-like repeat (SCR);
                     smart00032"
                     /db_xref="CDD:214478"
     Region          <748..779
                     /region_name="Sushi"
                     /note="Sushi repeat (SCR repeat); pfam00084"
                     /db_xref="CDD:459664"
     Region          <775..970
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          1069..1120
                     /region_name="Sushi"
                     /note="Sushi repeat (SCR repeat); pfam00084"
                     /db_xref="CDD:459664"
     CDS             1..1199
                     /gene="LOC144114394"
                     /coded_by="XM_077648120.1:140..3739"
                     /db_xref="GeneID:144114394"
ORIGIN      
        1 mrpplflama vlacaksalc qeakcpepkn myvhyrvqgg eefqeefpvg ttltyrcgpg
       61 yipreiktwn atcyqnydgs vdwnigkhdc rpvscgypgd vrygrlldav fvfprrvryq
      121 cnrgyrlegr eelycqsdaq wsapkpecri vecgplesig ngtvrlsgtv fgskaefscn
      181 hgfklvgnsa rtcgadgqws desphceevw cseemllefa qrngtvvvgd qpisgrqrsn
      241 trvthicppn tdlvgseint cqengawlfd ppackakciv qpgpgsqvqr rtsgwrtrfs
      301 vvesgtqvse fehnlfvecv ngyvlrsrrm evelplsckd gvwtpsgpwc iekpctihgp
      361 fpegveaeqy gevphgqevr frcmsgymmq nteneqhqpr clfgridgsy psckpekcia
      421 peltgtilqr kksiakltyw ttevlpgaev tdgdqnlfvk crqgyhllnt repetqvlck
      481 sgawqppppw caedgcriad lppvkngrpl gdgivapifl gtyacdpafe lhaagrirch
      541 qgqwlgspps cvskdesnrk rkvcklenkh srfkayhkgi pvtpegpfeh gtelvfhcep
      601 vgevrfegpr tarcidgkwd pqvplcyplk psdiavrfln aiytaprgiv yvdpnsyvvv
      661 dcwsnssvel slksddqsnv isstrirqnf mrmkfwlalg ksasvicrrq vtpdnprsii
      721 vrgqpqfwcp plssnsqglr mkrrtersyy ftcmegyqiv gepdlhcqkh gwsdpipkce
      781 pisgasning ashqteqtva qtahpagpsg atlstpertd sqsterapae tphstakvaa
      841 dpspphvvne neapktegeh gnratdspvl nprystqeqi gtqsgvskdl nagsnngstm
      901 epkpipgqra rappqdvnqt shskrmnsqt crqnttsdsc tdlgnrngpp vescegcdee
      961 ppiaesitan nasatrkrdk estrdasgsr atdhehstgg pwqdanttap paqvpgklsn
     1021 rqepheeqyl ststtttvkp tmsssteatm plssnekycl gkkffeiapp glvialnetq
     1081 vpenstfwaf cddgftlvgn vtavsclhdg twslppnitc ieacgnfdag pggpalegrk
     1141 esydlgdsit lsckngtelq prierllclg drwsenavpi cvpakvktng akneerkra
//
LOCUS       XP_077504247            1032 aa            linear   INV 25-NOV-2025
DEFINITION  sushi, von Willebrand factor type A, EGF and pentraxin
            domain-containing protein 1-like isoform X7 [Amblyomma americanum].
ACCESSION   XP_077504247
VERSION     XP_077504247.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648121.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1032
                     /product="sushi, von Willebrand factor type A, EGF and
                     pentraxin domain-containing protein 1-like isoform X7"
                     /calculated_mol_wt=114558
     Region          6..274
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     Region          <520..>620
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     Region          648..695
                     /region_name="CCP"
                     /note="Domain abundant in complement control proteins;
                     SUSHI repeat; short complement-like repeat (SCR);
                     smart00032"
                     /db_xref="CDD:214478"
     Region          <817..848
                     /region_name="Sushi"
                     /note="Sushi repeat (SCR repeat); pfam00084"
                     /db_xref="CDD:459664"
     Region          902..953
                     /region_name="Sushi"
                     /note="Sushi repeat (SCR repeat); pfam00084"
                     /db_xref="CDD:459664"
     CDS             1..1032
                     /gene="LOC144114394"
                     /coded_by="XM_077648121.1:140..3238"
                     /db_xref="GeneID:144114394"
ORIGIN      
        1 mrpplflama vlacaksalc qeakcpepkn myvhyrvqgg eefqeefpvg ttltyrcgpg
       61 yipreiktwn atcyqnydgs vdwnigkhdc rpvscgypgd vrygrlldav fvfprrvryq
      121 cnrgyrlegr eelycqsdaq wsapkpecri vecgplesig ngtvrlsgtv fgskaefscn
      181 hgfklvgnsa rtcgadgqws desphceevw cseemllefa qrngtvvvgd qpisgrqrsn
      241 trvthicppn tdlvgseint cqengawlfd ppackakciv qpgpgsqvqr rtsgwrtrfs
      301 vvesgtqvse fehnlfvecv ngyvlrsrrm evelplsckd gvwtpsgpwc iekpctihgp
      361 fpegveaeqy gevphgqevr frcmsgymmq nteneqhqpr clfgridgsy psckpddcri
      421 edlpfvengr lragqsvshg slaiyecnps lelqqrgggv rchfgqwigt splcvpkkci
      481 apeltgtilq rkksiakggd mrersltywt tevlpgaevt dgdqnlfvkc rqgyhllntr
      541 epetqvlcks gawqppppwc aedgcriadl ppvkngrplg dgivapiflg tyacdpafel
      601 haagrirchq gqwlgsppsc vskdesnrkr kvcklenkhs rfkayhkgip vtpegpfehg
      661 telvfhcepv gevrfegprt arcidgkwdp qvplcyplkp sdiavrflna iytaprgivy
      721 vdpnsyvvvd cwsnssvels lksddqsnvi sstrirqnfm rmkfwlalgk sasvicrrqv
      781 tpdnprsiiv rgqpqfwcpp lssnsqglrm krrtersyyf tcmegyqivg epdlhcqkhg
      841 wsdpipkcep isgasninga shqteqtvaq tahpagpsga tlstpertek yclgkkffei
      901 appglvialn etqvpenstf wafcddgftl vgnvtavscl hdgtwslppn itcieacgnf
      961 dagpggpale grkesydlgd sitlsckngt elqprierll clgdrwsena vpicvpakvk
     1021 tngakneerk ra
//
LOCUS       XP_077504248             153 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114395 [Amblyomma americanum].
ACCESSION   XP_077504248
VERSION     XP_077504248.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648122.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..153
                     /product="uncharacterized protein LOC144114395"
                     /calculated_mol_wt=16994
     Region          68..>117
                     /region_name="BEN"
                     /note="The BEN domain is found in diverse animal proteins;
                     smart01025"
                     /db_xref="CDD:214981"
     CDS             1..153
                     /gene="LOC144114395"
                     /coded_by="XM_077648122.1:2688..3149"
                     /db_xref="GeneID:144114395"
ORIGIN      
        1 mlkrlqqavn rltsaaegak psepeentir vvlqklaqcm hryigggvmv sgatitmlqe
       61 sfrdasckfa ralmrvvftd eeltnktlfg rqanihkeyp vkaaldqrrv savleytcck
      121 frcsdvklkr slgsmlqkmg tkrdtsskes sge
//
LOCUS       XP_077504249             153 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114395 [Amblyomma americanum].
ACCESSION   XP_077504249
VERSION     XP_077504249.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648123.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..153
                     /product="uncharacterized protein LOC144114395"
                     /calculated_mol_wt=16994
     Region          68..>117
                     /region_name="BEN"
                     /note="The BEN domain is found in diverse animal proteins;
                     smart01025"
                     /db_xref="CDD:214981"
     CDS             1..153
                     /gene="LOC144114395"
                     /coded_by="XM_077648123.1:1375..1836"
                     /db_xref="GeneID:144114395"
ORIGIN      
        1 mlkrlqqavn rltsaaegak psepeentir vvlqklaqcm hryigggvmv sgatitmlqe
       61 sfrdasckfa ralmrvvftd eeltnktlfg rqanihkeyp vkaaldqrrv savleytcck
      121 frcsdvklkr slgsmlqkmg tkrdtsskes sge
//
LOCUS       XP_077504250             244 aa            linear   INV 25-NOV-2025
DEFINITION  L-xylulose reductase-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504250
VERSION     XP_077504250.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648124.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..244
                     /product="L-xylulose reductase-like isoform X1"
                     /calculated_mol_wt=25543
     Region          1..244
                     /region_name="NADB_Rossmann"
                     /note="Rossmann-fold NAD(P)(+)-binding proteins; cl21454"
                     /db_xref="CDD:473865"
     Site            order(14,16..17,19,38..40,83..85,134..136,149,153,
                     179..182)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:187535"
     Site            order(107,136,149,153)
                     /site_type="active"
                     /db_xref="CDD:187535"
     CDS             1..244
                     /gene="LOC144114397"
                     /coded_by="XM_077648124.1:280..1014"
                     /db_xref="GeneID:144114397"
ORIGIN      
        1 mevsfggkra vvtgasqgig rviarelakr gatviavars adkleqlqke vpnivplpvd
       61 ladwnateaa lsnvgpvdll vnnaavaile pvgeirpesi dmcfttnvka vinvsqictk
      121 smkergvaga ivnissqagi valadhavyc atkaaldqlt rvmalelgpy kirvnsvnpt
      181 vtntpmslvg wsdpvkasam qskiplgrfa epeevcdavl yllserssmi tgtvlpidgg
      241 ytac
//
LOCUS       XP_077504251             227 aa            linear   INV 25-NOV-2025
DEFINITION  L-xylulose reductase-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504251
VERSION     XP_077504251.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648125.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..227
                     /product="L-xylulose reductase-like isoform X2"
                     /calculated_mol_wt=23703
     Region          1..214
                     /region_name="NADB_Rossmann"
                     /note="Rossmann-fold NAD(P)(+)-binding proteins; cl21454"
                     /db_xref="CDD:473865"
     Site            order(14,16..17,19,38..40,83..85,134..136,149,153,
                     179..182)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:187535"
     Site            order(107,136,149,153)
                     /site_type="active"
                     /db_xref="CDD:187535"
     CDS             1..227
                     /gene="LOC144114397"
                     /coded_by="XM_077648125.1:270..953"
                     /db_xref="GeneID:144114397"
ORIGIN      
        1 mevsfggkra vvtgasqgig rviarelakr gatviavars adkleqlqke vpnivplpvd
       61 ladwnateaa lsnvgpvdll vnnaavaile pvgeirpesi dmcfttnvka vinvsqictk
      121 smkergvaga ivnissqagi valadhavyc atkaaldqlt rvmalelgpy kirvnsvnpt
      181 vtntpmslvg wsdpvkasam qskiplgrfa gvtekhrdgt papiapf
//
LOCUS       XP_077504253             948 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: protein FAM13A [Amblyomma americanum].
ACCESSION   XP_077504253
VERSION     XP_077504253.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648127.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..948
                     /product="LOW QUALITY PROTEIN: protein FAM13A"
                     /calculated_mol_wt=106330
     Region          52..236
                     /region_name="RhoGAP"
                     /note="GTPase-activator protein (GAP) for Rho-like
                     GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases.
                     Small GTPases (G proteins) cluster into distinct families,
                     and all act as molecular switches, active in their
                     GTP-bound form but inactive when bound to...; cl02570"
                     /db_xref="CDD:470621"
     Site            order(90,127,131,202,205..206,228)
                     /site_type="other"
                     /note="GTPase interaction site"
                     /db_xref="CDD:238090"
     Site            90
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:238090"
     Region          623..665
                     /region_name="RecQL4_SLD2_NTD"
                     /note="N-terminal homeodomain-like domain of metazoan RecQ
                     protein-like 4 (RecQL4), fungal DNA replication regulator
                     SLD2 and similar proteins; cd22289"
                     /db_xref="CDD:412085"
     CDS             1..948
                     /gene="LOC144114405"
                     /coded_by="XM_077648127.1:562..3408"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:144114405"
ORIGIN      
        1 mrkptfctdv tvaemtsanq nlevcgesrm ervrkllgsp larrksgala lrifgaplds
       61 laqkgsvpfv vvrlcqyien tgmgqeglfr vsgnarlvek lrwsfdqsgd apleaegdva
      121 aaaallkllf relpeplvpm elhqaflnai qatvhdkeec trrlkylveq lppsnfavlk
      181 ylchflsrva lleehtrmtp salgivfgpn ifrfsedlqg lkeqavtnqv vtyfidhyns
      241 ifegdaelsc ttdaamsiqn ggsstsnkcs grvitgisva smqpltvvip pldeamlalk
      301 qdtlnevptl midsliddrc nqaimyrvak egnsngtalp srsckrkerr isgedtpapr
      361 ssseerpaag lrvepelasr rcssheevtt leavqgyssq mapgggsgrn gtsgddasrd
      421 lsphppqpkr reietpsspr pdnsqewrea crnverpnpl hlqdvqyqaa pdeassdrpm
      481 vdveepcgpp cdgreaggsp eevdgsvpnt pstiskdsip pldlnsfree epvlsehrfs
      541 wpmvqalade epvmpylqka nsyeaplsps ayrsylshrs lhldpnlpps ppveqdeflk
      601 ssfkdeeasq ttvkqlskrv hclkkkvkqf eenfekehgy rpshaekmnh aemkrtllel
      661 nklrkdlknl keenslpepa ylsfsswsre slrgladmdd gvakpthime kalndtlmcl
      721 aekrraaqrp dsvddmtreq vleeklalqk allrfesvhg rpsrradrnl mrplydryrs
      781 vkrlvgrnpl akgkdselqp ilehvamnft spqprsppqe leevveivtp vsavaqseai
      841 teleepvtsk qevveqvstv lekcsdsnlh elplaellqq qqqsklekrr lrkmlrefee
      901 dfqkqtgrkv qkedkapmds vyceykhvka rmrllevlvs khehrrei
//
LOCUS       XP_077504254             175 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114407 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504254
VERSION     XP_077504254.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648128.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..175
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..175
                     /product="uncharacterized protein LOC144114407 isoform X1"
                     /calculated_mol_wt=19008
     CDS             1..175
                     /gene="LOC144114407"
                     /coded_by="XM_077648128.1:224..751"
                     /db_xref="GeneID:144114407"
ORIGIN      
        1 masyrelhtv grggsrvklc esigcglptk kqmdqlnrid ervrnveelr aqgvmspira
       61 qssvrpqhep fprsshltss mtppssgsem lptngavsgq kqspkktlkr eleftsspsq
      121 tssvsatglt rgsfipsgat ltpqdpalis tganphykqn ektrrneers fcrls
//
LOCUS       XP_077504255             143 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114407 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504255
VERSION     XP_077504255.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648129.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..143
                     /product="uncharacterized protein LOC144114407 isoform X2"
                     /calculated_mol_wt=15563
     CDS             1..143
                     /gene="LOC144114407"
                     /coded_by="XM_077648129.1:261..692"
                     /db_xref="GeneID:144114407"
ORIGIN      
        1 mdqlnrider vrnveelraq gvmspiraqs svrpqhepfp rsshltssmt ppssgsemlp
       61 tngavsgqkq spkktlkrel eftsspsqts svsatgltrg sfipsgatlt pqdpalistg
      121 anphykqnek trrneersfc rls
//
LOCUS       XP_077504256             143 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114407 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504256
VERSION     XP_077504256.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648130.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..143
                     /product="uncharacterized protein LOC144114407 isoform X2"
                     /calculated_mol_wt=15563
     CDS             1..143
                     /gene="LOC144114407"
                     /coded_by="XM_077648130.1:169..600"
                     /db_xref="GeneID:144114407"
ORIGIN      
        1 mdqlnrider vrnveelraq gvmspiraqs svrpqhepfp rsshltssmt ppssgsemlp
       61 tngavsgqkq spkktlkrel eftsspsqts svsatgltrg sfipsgatlt pqdpalistg
      121 anphykqnek trrneersfc rls
//
LOCUS       XP_077504257             406 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and
            dual-specificity protein phosphatase PTEN-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077504257
VERSION     XP_077504257.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648131.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..406
                     /product="phosphatidylinositol 3,4,5-trisphosphate
                     3-phosphatase and dual-specificity protein phosphatase
                     PTEN-like isoform X1"
                     /calculated_mol_wt=45631
     Region          26..183
                     /region_name="PTP_PTEN"
                     /note="protein tyrosine phosphatase-like catalytic domain
                     of phosphatase and tensin homolog; cd14509"
                     /db_xref="CDD:350359"
     Region          190..367
                     /region_name="PTEN_C2"
                     /note="C2 domain of PTEN tumour-suppressor protein;
                     pfam10409"
                     /db_xref="CDD:463081"
     CDS             1..406
                     /gene="LOC144114410"
                     /coded_by="XM_077648131.1:121..1341"
                     /db_xref="GeneID:144114410"
ORIGIN      
        1 mtmtarikqv vsknkkryqd edfdldlsyi sdniiamgfp aeklegvfrn hmddvqkfle
       61 kkhrghykiy nlcserqyda kkfenrvahy pfkdhnppki dlikpfcedv erwlsshsen
      121 vavihckagk grtgvmvcsy lvhcrmftta rdalsyyser rttdlkgvti psqrryveyy
      181 aklvsmheey npetlelhsl slepvptfng gtcslffvlw ksgatsleql ymspvkegrk
      241 gnstplrfpl ectvtlkgdi kvevynkpkm mkkekvfqfw fntffvprns sgsssggsgv
      301 gqgdahphpn gvaggggaap aelrvhngge eevrtlvlck ddldkaykdk thskfskdfk
      361 vslkfgprnq lgpsaaggdd aggsdfgtes dttddddwdt qeathi
//
LOCUS       XP_077504258             431 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and
            dual-specificity protein phosphatase PTEN-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077504258
VERSION     XP_077504258.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648132.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..431
                     /product="phosphatidylinositol 3,4,5-trisphosphate
                     3-phosphatase and dual-specificity protein phosphatase
                     PTEN-like isoform X2"
                     /calculated_mol_wt=48699
     Region          26..183
                     /region_name="PTP_PTEN"
                     /note="protein tyrosine phosphatase-like catalytic domain
                     of phosphatase and tensin homolog; cd14509"
                     /db_xref="CDD:350359"
     Region          190..392
                     /region_name="PTEN_C2"
                     /note="C2 domain of PTEN tumour-suppressor protein;
                     pfam10409"
                     /db_xref="CDD:463081"
     CDS             1..431
                     /gene="LOC144114410"
                     /coded_by="XM_077648132.1:166..1461"
                     /db_xref="GeneID:144114410"
ORIGIN      
        1 mtmtarikqv vsknkkryqd edfdldlsyi sdniiamgfp aeklegvfrn hmddvqkfle
       61 kkhrghykiy nlcserqyda kkfenrvahy pfkdhnppki dlikpfcedv erwlsshsen
      121 vavihckagk grtgvmvcsy lvhcrmftta rdalsyyser rttdlkgvti psqrryveyy
      181 aklvsmheey npetlelhsl slepvptfng gtcslffvlw ksgatsleql ymspvkegrk
      241 gnstplrfpl ectvtlkgdi kvevynkpkm mkkekvfqfw fntffvprns sgsssggsgv
      301 gqgdahphpn gvaggggaap aelrraktra rslykqymqa tsnirkcrrv hnggeeevrt
      361 lvlckddldk aykdkthskf skdfkvslkf gprnqlgpsa aggddaggsd fgtesdttdd
      421 ddwdtqeath i
//
LOCUS       XP_077504259             431 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and
            dual-specificity protein phosphatase PTEN-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077504259
VERSION     XP_077504259.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648133.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..431
                     /product="phosphatidylinositol 3,4,5-trisphosphate
                     3-phosphatase and dual-specificity protein phosphatase
                     PTEN-like isoform X2"
                     /calculated_mol_wt=48699
     Region          26..183
                     /region_name="PTP_PTEN"
                     /note="protein tyrosine phosphatase-like catalytic domain
                     of phosphatase and tensin homolog; cd14509"
                     /db_xref="CDD:350359"
     Region          190..392
                     /region_name="PTEN_C2"
                     /note="C2 domain of PTEN tumour-suppressor protein;
                     pfam10409"
                     /db_xref="CDD:463081"
     CDS             1..431
                     /gene="LOC144114410"
                     /coded_by="XM_077648133.1:211..1506"
                     /db_xref="GeneID:144114410"
ORIGIN      
        1 mtmtarikqv vsknkkryqd edfdldlsyi sdniiamgfp aeklegvfrn hmddvqkfle
       61 kkhrghykiy nlcserqyda kkfenrvahy pfkdhnppki dlikpfcedv erwlsshsen
      121 vavihckagk grtgvmvcsy lvhcrmftta rdalsyyser rttdlkgvti psqrryveyy
      181 aklvsmheey npetlelhsl slepvptfng gtcslffvlw ksgatsleql ymspvkegrk
      241 gnstplrfpl ectvtlkgdi kvevynkpkm mkkekvfqfw fntffvprns sgsssggsgv
      301 gqgdahphpn gvaggggaap aelrraktra rslykqymqa tsnirkcrrv hnggeeevrt
      361 lvlckddldk aykdkthskf skdfkvslkf gprnqlgpsa aggddaggsd fgtesdttdd
      421 ddwdtqeath i
//
LOCUS       XP_077504260             406 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and
            dual-specificity protein phosphatase PTEN-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077504260
VERSION     XP_077504260.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648134.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..406
                     /product="phosphatidylinositol 3,4,5-trisphosphate
                     3-phosphatase and dual-specificity protein phosphatase
                     PTEN-like isoform X1"
                     /calculated_mol_wt=45631
     Region          26..183
                     /region_name="PTP_PTEN"
                     /note="protein tyrosine phosphatase-like catalytic domain
                     of phosphatase and tensin homolog; cd14509"
                     /db_xref="CDD:350359"
     Region          190..367
                     /region_name="PTEN_C2"
                     /note="C2 domain of PTEN tumour-suppressor protein;
                     pfam10409"
                     /db_xref="CDD:463081"
     CDS             1..406
                     /gene="LOC144114410"
                     /coded_by="XM_077648134.1:206..1426"
                     /db_xref="GeneID:144114410"
ORIGIN      
        1 mtmtarikqv vsknkkryqd edfdldlsyi sdniiamgfp aeklegvfrn hmddvqkfle
       61 kkhrghykiy nlcserqyda kkfenrvahy pfkdhnppki dlikpfcedv erwlsshsen
      121 vavihckagk grtgvmvcsy lvhcrmftta rdalsyyser rttdlkgvti psqrryveyy
      181 aklvsmheey npetlelhsl slepvptfng gtcslffvlw ksgatsleql ymspvkegrk
      241 gnstplrfpl ectvtlkgdi kvevynkpkm mkkekvfqfw fntffvprns sgsssggsgv
      301 gqgdahphpn gvaggggaap aelrvhngge eevrtlvlck ddldkaykdk thskfskdfk
      361 vslkfgprnq lgpsaaggdd aggsdfgtes dttddddwdt qeathi
//
LOCUS       XP_077504261             192 aa            linear   INV 25-NOV-2025
DEFINITION  secretion-associated Ras-related 1 [Amblyomma americanum].
ACCESSION   XP_077504261
VERSION     XP_077504261.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648135.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..192
                     /product="secretion-associated Ras-related 1"
                     /calculated_mol_wt=21681
     Region          2..192
                     /region_name="Sar1"
                     /note="Sar1 is an essential component of COPII vesicle
                     coats; cd00879"
                     /db_xref="CDD:206645"
     Site            2..4
                     /site_type="active"
                     /note="STAR motif [active]"
                     /db_xref="CDD:206645"
     Site            27..34
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206645"
     Site            order(29..35,51,73,129..130,132,174..176)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206645"
     Site            order(29..30,34,38,45..57,79,84)
                     /site_type="other"
                     /note="GAP interaction site [polypeptide binding]"
                     /db_xref="CDD:206645"
     Site            39..54
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206645"
     Site            order(51..55,60,70,74,80,82..84)
                     /site_type="other"
                     /note="putative GEF interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206645"
     Site            51
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206645"
     Site            order(52..59,69,80,83..84)
                     /site_type="active"
                     /note="putative effector interaction site [active]"
                     /db_xref="CDD:206645"
     Site            55..69
                     /site_type="active"
                     /note="interswitch region [active]"
                     /db_xref="CDD:206645"
     Site            70..87
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206645"
     Site            70..73
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206645"
     Site            129..132
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206645"
     Site            174..176
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206645"
     CDS             1..192
                     /gene="Sar1"
                     /coded_by="XM_077648135.1:170..748"
                     /db_xref="GeneID:144114411"
ORIGIN      
        1 mfllewlggv lgylglwkks gkllflgldn agkttllhml kddrmaqhvp tlhptseels
       61 ignmrfttfd lgghhqarrv wkdyfpavda ivflidahdr erfpeskael dslltdeqla
      121 ncpvlilgnk idkpgaased elrtyfclys qttgkgkvpr semtarplem fmcsvlkrqg
      181 ygegfrwlaq yi
//
LOCUS       XP_077504262             192 aa            linear   INV 25-NOV-2025
DEFINITION  secretion-associated Ras-related 1 [Amblyomma americanum].
ACCESSION   XP_077504262
VERSION     XP_077504262.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648136.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..192
                     /product="secretion-associated Ras-related 1"
                     /calculated_mol_wt=21681
     Region          2..192
                     /region_name="Sar1"
                     /note="Sar1 is an essential component of COPII vesicle
                     coats; cd00879"
                     /db_xref="CDD:206645"
     Site            2..4
                     /site_type="active"
                     /note="STAR motif [active]"
                     /db_xref="CDD:206645"
     Site            27..34
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206645"
     Site            order(29..35,51,73,129..130,132,174..176)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206645"
     Site            order(29..30,34,38,45..57,79,84)
                     /site_type="other"
                     /note="GAP interaction site [polypeptide binding]"
                     /db_xref="CDD:206645"
     Site            39..54
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206645"
     Site            order(51..55,60,70,74,80,82..84)
                     /site_type="other"
                     /note="putative GEF interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206645"
     Site            51
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206645"
     Site            order(52..59,69,80,83..84)
                     /site_type="active"
                     /note="putative effector interaction site [active]"
                     /db_xref="CDD:206645"
     Site            55..69
                     /site_type="active"
                     /note="interswitch region [active]"
                     /db_xref="CDD:206645"
     Site            70..87
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206645"
     Site            70..73
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206645"
     Site            129..132
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206645"
     Site            174..176
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206645"
     CDS             1..192
                     /gene="Sar1"
                     /coded_by="XM_077648136.1:39..617"
                     /db_xref="GeneID:144114411"
ORIGIN      
        1 mfllewlggv lgylglwkks gkllflgldn agkttllhml kddrmaqhvp tlhptseels
       61 ignmrfttfd lgghhqarrv wkdyfpavda ivflidahdr erfpeskael dslltdeqla
      121 ncpvlilgnk idkpgaased elrtyfclys qttgkgkvpr semtarplem fmcsvlkrqg
      181 ygegfrwlaq yi
//
LOCUS       XP_077504263             172 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial ribosomal protein S24 [Amblyomma americanum].
ACCESSION   XP_077504263
VERSION     XP_077504263.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648137.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..172
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..172
                     /product="mitochondrial ribosomal protein S24"
                     /calculated_mol_wt=19698
     Region          38..172
                     /region_name="MRP-S24"
                     /note="Mitochondrial ribosome subunit S24; pfam14955"
                     /db_xref="CDD:464401"
     CDS             1..172
                     /gene="mRpS24"
                     /coded_by="XM_077648137.1:44..562"
                     /db_xref="GeneID:144114412"
ORIGIN      
        1 maapmsalck algrlcrpei llspgrgaal httatleknr agryrvtrnh srpltyeman
       61 pphqiahrka wnswntsnlq gglrrsetlv edvfirkfmt gtwhrlfvse ilikrranmv
      121 figgivqrvv iprkmyflig yteeilsyil kcpvklelqs vadrkdmifk yi
//
LOCUS       XP_077504264             700 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: cocaine esterase-like [Amblyomma americanum].
ACCESSION   XP_077504264
VERSION     XP_077504264.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648138.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..700
                     /product="LOW QUALITY PROTEIN: cocaine esterase-like"
                     /calculated_mol_wt=76412
     Region          91..613
                     /region_name="COesterase"
                     /note="Carboxylesterase family; pfam00135"
                     /db_xref="CDD:395084"
     Site            order(193..195,271..273,276,428,432..433,475,530,533)
                     /site_type="other"
                     /note="substrate binding pocket [chemical binding]"
                     /db_xref="CDD:238191"
     Site            order(272,402,529)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238191"
     CDS             1..700
                     /gene="LOC144114413"
                     /coded_by="XM_077648138.1:112..2214"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:144114413"
ORIGIN      
        1 mmiapapppl laaallllll lvgrtqqqqq qqqnfprtrf qpvhrypplp dgiklspeli
       61 nqalqlppns nghpqataqq qggfrpqrmv qltipqgvlr grvlrtpsgr glvaflgvpy
      121 aaaplgplrf kppvihpgws svldatafrs acpqfdyrgr ivgnedclfl niytpgyrng
      181 qtpsypvmvf vhggnfetga asqygpqklv dkdvvvvtln yrlgilgfls tgdsvcpgnl
      241 glldqnlalk wvrdnvahfg gdpsqvtlfg qgsgavsvfl hilsplsqgl faraiaesgs
      301 plsdwsiepd paqfkrtvaq gagcpadgps falidclmqt psseflriqq eskvfgdfpv
      361 rtapvvekfn pqgaflpdep aallergefr rvpliagvnk detafffpvl saylrdrsar
      421 satyvrdqll prflssalry srpppdvvds vdetyfrdin pldtgaiira finmstdamf
      481 vagnqaalak ysrfeaptym yvfeyrgdts lldarlgfqr qsydlgvsng deliylfdvt
      541 adglrplssl dslvssriis lwtdfaksgm apqypnfeyp awpratpsnv sayrigrslq
      601 pgpeyrveat rfwsgispal lgqragesdd srsgalatps gyveplyrtl awsmvavaia
      661 lalalivllv vlynqkksqs frassdldns rmstggstly
//
LOCUS       XP_077504265             701 aa            linear   INV 25-NOV-2025
DEFINITION  glutamine--fructose-6-phosphate aminotransferase [isomerizing]
            2-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504265
VERSION     XP_077504265.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648139.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..701
                     /product="glutamine--fructose-6-phosphate aminotransferase
                     [isomerizing] 2-like isoform X1"
                     /calculated_mol_wt=78922
     Region          1..701
                     /region_name="PLN02981"
                     /note="glucosamine:fructose-6-phosphate aminotransferase"
                     /db_xref="CDD:215531"
     CDS             1..701
                     /gene="LOC144114414"
                     /coded_by="XM_077648139.1:208..2313"
                     /db_xref="GeneID:144114414"
ORIGIN      
        1 mcgifaylny lepktrreil eclikglqrl eyrgydsagv afdgdaqgnn isiirkigkv
       61 kvledsiwrk ddldmdhehl thigiahtrw athgapsdln shpqrsdpen efvvvhngii
      121 tnykdikqfl iqkgcrfesd tdteviakfi khihnthpdl sfrelveqvi qqlegafalv
      181 fkskkfphqc vatrrgspll vglktksyls tefipvlysk altvdhkcgs qndeekddtq
      241 tdhrpreshp psntgglqrt asttefgppg ddkeieyffa sdasaiieht nrvifleddd
      301 vaavseghlt ihrlkrtlde nvnreiitlk meiqqimkgn fssfmqkeif eqpesvintm
      361 rgrlsfeket vvlggikdyi teikrcrrll ligcgtsyhs aiatrqilee ltelpvmvel
      421 asdfldrntp vfrddvcffi sqsgetadtl malryckqrg alivgitntv gssicreshc
      481 gvhinagpei gvastkayts qfvslvmfsl mmcedrismq prrteiiqgl hrlpdqikev
      541 lkldnevkql aqhlyqqksl lvmgrgynha tclegalkik eltymhsegi lagelkhgpl
      601 alvdkampvm mvltrdpvfp kcmnalqqvi ardgrpiiic ekgdeetkkl afkylevpht
      661 vdclqgvltv iplqlmsyhi avlkgcnvdc prnlaksvtv e
//
LOCUS       XP_077504266             696 aa            linear   INV 25-NOV-2025
DEFINITION  glutamine--fructose-6-phosphate aminotransferase [isomerizing]
            2-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504266
VERSION     XP_077504266.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648140.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..696
                     /product="glutamine--fructose-6-phosphate aminotransferase
                     [isomerizing] 2-like isoform X2"
                     /calculated_mol_wt=78077
     Region          1..696
                     /region_name="PLN02981"
                     /note="glucosamine:fructose-6-phosphate aminotransferase"
                     /db_xref="CDD:215531"
     CDS             1..696
                     /gene="LOC144114414"
                     /coded_by="XM_077648140.1:219..2309"
                     /db_xref="GeneID:144114414"
ORIGIN      
        1 mcgifaylny lepktrreil eclikglqrl eyrgydsagv afdgdaqgnn isiirkigkv
       61 kvledsiwrk ddldmdhehl thigiahtrw athgapsdln shpqrsdpen efvvvhngii
      121 tnykdikqfl iqkgcrfesd tdteviakfi khihnthpdl sfrelveqvi qqlegafalv
      181 fkskkfphqc vatrrgspll vglktksyls tefipvlysk gggfsfgtga ltcplgyhrp
      241 reshppsntg glqrtastte fgppgddkei eyffasdasa iiehtnrvif ledddvaavs
      301 eghltihrlk rtldenvnre iitlkmeiqq imkgnfssfm qkeifeqpes vintmrgrls
      361 feketvvlgg ikdyiteikr crrllligcg tsyhsaiatr qileeltelp vmvelasdfl
      421 drntpvfrdd vcffisqsge tadtlmalry ckqrgalivg itntvgssic reshcgvhin
      481 agpeigvast kaytsqfvsl vmfslmmced rismqprrte iiqglhrlpd qikevlkldn
      541 evkqlaqhly qqksllvmgr gynhatcleg alkikeltym hsegilagel khgplalvdk
      601 ampvmmvltr dpvfpkcmna lqqviardgr piiicekgde etkklafkyl evphtvdclq
      661 gvltviplql msyhiavlkg cnvdcprnla ksvtve
//
LOCUS       XP_077504267             695 aa            linear   INV 25-NOV-2025
DEFINITION  glutamine--fructose-6-phosphate aminotransferase [isomerizing]
            2-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077504267
VERSION     XP_077504267.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648141.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..695
                     /product="glutamine--fructose-6-phosphate aminotransferase
                     [isomerizing] 2-like isoform X3"
                     /calculated_mol_wt=78286
     Region          1..695
                     /region_name="PLN02981"
                     /note="glucosamine:fructose-6-phosphate aminotransferase"
                     /db_xref="CDD:215531"
     CDS             1..695
                     /gene="LOC144114414"
                     /coded_by="XM_077648141.1:208..2295"
                     /db_xref="GeneID:144114414"
ORIGIN      
        1 mcgifaylny lepktrreil eclikglqrl eyrgydsagv afdgdaqgnn isiirkigkv
       61 kvledsiwrk ddldmdhehl thigiahtrw athgapsdln shpqrsdpen efvvvhngii
      121 tnykdikqfl iqkgcrfesd tdteviakfi khihnthpdl sfrelveqvi qqlegafalv
      181 fkskkfphqc vatrrgspll vglktksyls tefipvlysk ecgsqndeek ddtqtdhrpr
      241 eshppsntgg lqrtasttef gppgddkeie yffasdasai iehtnrvifl edddvaavse
      301 ghltihrlkr tldenvnrei itlkmeiqqi mkgnfssfmq keifeqpesv intmrgrlsf
      361 eketvvlggi kdyiteikrc rrllligcgt syhsaiatrq ileeltelpv mvelasdfld
      421 rntpvfrddv cffisqsget adtlmalryc kqrgalivgi tntvgssicr eshcgvhina
      481 gpeigvastk aytsqfvslv mfslmmcedr ismqprrtei iqglhrlpdq ikevlkldne
      541 vkqlaqhlyq qksllvmgrg ynhatclega lkikeltymh segilagelk hgplalvdka
      601 mpvmmvltrd pvfpkcmnal qqviardgrp iiicekgdee tkklafkyle vphtvdclqg
      661 vltviplqlm syhiavlkgc nvdcprnlak svtve
//
LOCUS       XP_077504268             694 aa            linear   INV 25-NOV-2025
DEFINITION  glutamine--fructose-6-phosphate aminotransferase [isomerizing]
            2-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077504268
VERSION     XP_077504268.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648142.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..694
                     /product="glutamine--fructose-6-phosphate aminotransferase
                     [isomerizing] 2-like isoform X4"
                     /calculated_mol_wt=78104
     Region          1..694
                     /region_name="PLN02981"
                     /note="glucosamine:fructose-6-phosphate aminotransferase"
                     /db_xref="CDD:215531"
     CDS             1..694
                     /gene="LOC144114414"
                     /coded_by="XM_077648142.1:208..2292"
                     /db_xref="GeneID:144114414"
ORIGIN      
        1 mcgifaylny lepktrreil eclikglqrl eyrgydsagv afdgdaqgnn isiirkigkv
       61 kvledsiwrk ddldmdhehl thigiahtrw athgapsdln shpqrsdpen efvvvhngii
      121 tnykdikqfl iqkgcrfesd tdteviakfi khihnthpdl sfrelveqvi qqlegafalv
      181 fkskkfphqc vatrrgspll vglktksyls tefipvlysk altvdhkcgs qndedhrpre
      241 shppsntggl qrtasttefg ppgddkeiey ffasdasaii ehtnrvifle dddvaavseg
      301 hltihrlkrt ldenvnreii tlkmeiqqim kgnfssfmqk eifeqpesvi ntmrgrlsfe
      361 ketvvlggik dyiteikrcr rllligcgts yhsaiatrqi leeltelpvm velasdfldr
      421 ntpvfrddvc ffisqsgeta dtlmalryck qrgalivgit ntvgssicre shcgvhinag
      481 peigvastka ytsqfvslvm fslmmcedri smqprrteii qglhrlpdqi kevlkldnev
      541 kqlaqhlyqq ksllvmgrgy nhatclegal kikeltymhs egilagelkh gplalvdkam
      601 pvmmvltrdp vfpkcmnalq qviardgrpi iicekgdeet kklafkylev phtvdclqgv
      661 ltviplqlms yhiavlkgcn vdcprnlaks vtve
//
LOCUS       XP_077504269             688 aa            linear   INV 25-NOV-2025
DEFINITION  glutamine--fructose-6-phosphate aminotransferase [isomerizing]
            2-like isoform X5 [Amblyomma americanum].
ACCESSION   XP_077504269
VERSION     XP_077504269.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648143.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..688
                     /product="glutamine--fructose-6-phosphate aminotransferase
                     [isomerizing] 2-like isoform X5"
                     /calculated_mol_wt=77469
     Region          1..688
                     /region_name="PLN02981"
                     /note="glucosamine:fructose-6-phosphate aminotransferase"
                     /db_xref="CDD:215531"
     CDS             1..688
                     /gene="LOC144114414"
                     /coded_by="XM_077648143.1:208..2274"
                     /db_xref="GeneID:144114414"
ORIGIN      
        1 mcgifaylny lepktrreil eclikglqrl eyrgydsagv afdgdaqgnn isiirkigkv
       61 kvledsiwrk ddldmdhehl thigiahtrw athgapsdln shpqrsdpen efvvvhngii
      121 tnykdikqfl iqkgcrfesd tdteviakfi khihnthpdl sfrelveqvi qqlegafalv
      181 fkskkfphqc vatrrgspll vglktksyls tefipvlysk ecgsqndedh rpreshppsn
      241 tgglqrtast tefgppgddk eieyffasda saiiehtnrv ifledddvaa vseghltihr
      301 lkrtldenvn reiitlkmei qqimkgnfss fmqkeifeqp esvintmrgr lsfeketvvl
      361 ggikdyitei krcrrlllig cgtsyhsaia trqileelte lpvmvelasd fldrntpvfr
      421 ddvcffisqs getadtlmal ryckqrgali vgitntvgss icreshcgvh inagpeigva
      481 stkaytsqfv slvmfslmmc edrismqprr teiiqglhrl pdqikevlkl dnevkqlaqh
      541 lyqqksllvm grgynhatcl egalkikelt ymhsegilag elkhgplalv dkampvmmvl
      601 trdpvfpkcm nalqqviard grpiiicekg deetkklafk ylevphtvdc lqgvltvipl
      661 qlmsyhiavl kgcnvdcprn laksvtve
//
LOCUS       XP_077504270             687 aa            linear   INV 25-NOV-2025
DEFINITION  glutamine--fructose-6-phosphate aminotransferase [isomerizing]
            2-like isoform X6 [Amblyomma americanum].
ACCESSION   XP_077504270
VERSION     XP_077504270.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648144.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..687
                     /product="glutamine--fructose-6-phosphate aminotransferase
                     [isomerizing] 2-like isoform X6"
                     /calculated_mol_wt=77424
     Region          1..687
                     /region_name="PLN02981"
                     /note="glucosamine:fructose-6-phosphate aminotransferase"
                     /db_xref="CDD:215531"
     CDS             1..687
                     /gene="LOC144114414"
                     /coded_by="XM_077648144.1:208..2271"
                     /db_xref="GeneID:144114414"
ORIGIN      
        1 mcgifaylny lepktrreil eclikglqrl eyrgydsagv afdgdaqgnn isiirkigkv
       61 kvledsiwrk ddldmdhehl thigiahtrw athgapsdln shpqrsdpen efvvvhngii
      121 tnykdikqfl iqkgcrfesd tdteviakfi khihnthpdl sfrelveqvi qqlegafalv
      181 fkskkfphqc vatrrgspll vglktksyls tefipvlysk ekddtqtdhr preshppsnt
      241 gglqrtastt efgppgddke ieyffasdas aiiehtnrvi fledddvaav seghltihrl
      301 krtldenvnr eiitlkmeiq qimkgnfssf mqkeifeqpe svintmrgrl sfeketvvlg
      361 gikdyiteik rcrrllligc gtsyhsaiat rqileeltel pvmvelasdf ldrntpvfrd
      421 dvcffisqsg etadtlmalr yckqrgaliv gitntvgssi creshcgvhi nagpeigvas
      481 tkaytsqfvs lvmfslmmce drismqprrt eiiqglhrlp dqikevlkld nevkqlaqhl
      541 yqqksllvmg rgynhatcle galkikelty mhsegilage lkhgplalvd kampvmmvlt
      601 rdpvfpkcmn alqqviardg rpiiicekgd eetkklafky levphtvdcl qgvltviplq
      661 lmsyhiavlk gcnvdcprnl aksvtve
//
LOCUS       XP_077504271             686 aa            linear   INV 25-NOV-2025
DEFINITION  glutamine--fructose-6-phosphate aminotransferase [isomerizing]
            2-like isoform X7 [Amblyomma americanum].
ACCESSION   XP_077504271
VERSION     XP_077504271.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648145.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..686
                     /product="glutamine--fructose-6-phosphate aminotransferase
                     [isomerizing] 2-like isoform X7"
                     /calculated_mol_wt=77241
     Region          1..686
                     /region_name="PLN02981"
                     /note="glucosamine:fructose-6-phosphate aminotransferase"
                     /db_xref="CDD:215531"
     CDS             1..686
                     /gene="LOC144114414"
                     /coded_by="XM_077648145.1:208..2268"
                     /db_xref="GeneID:144114414"
ORIGIN      
        1 mcgifaylny lepktrreil eclikglqrl eyrgydsagv afdgdaqgnn isiirkigkv
       61 kvledsiwrk ddldmdhehl thigiahtrw athgapsdln shpqrsdpen efvvvhngii
      121 tnykdikqfl iqkgcrfesd tdteviakfi khihnthpdl sfrelveqvi qqlegafalv
      181 fkskkfphqc vatrrgspll vglktksyls tefipvlysk altvdhnhrp reshppsntg
      241 glqrtastte fgppgddkei eyffasdasa iiehtnrvif ledddvaavs eghltihrlk
      301 rtldenvnre iitlkmeiqq imkgnfssfm qkeifeqpes vintmrgrls feketvvlgg
      361 ikdyiteikr crrllligcg tsyhsaiatr qileeltelp vmvelasdfl drntpvfrdd
      421 vcffisqsge tadtlmalry ckqrgalivg itntvgssic reshcgvhin agpeigvast
      481 kaytsqfvsl vmfslmmced rismqprrte iiqglhrlpd qikevlkldn evkqlaqhly
      541 qqksllvmgr gynhatcleg alkikeltym hsegilagel khgplalvdk ampvmmvltr
      601 dpvfpkcmna lqqviardgr piiicekgde etkklafkyl evphtvdclq gvltviplql
      661 msyhiavlkg cnvdcprnla ksvtve
//
LOCUS       XP_077504272             680 aa            linear   INV 25-NOV-2025
DEFINITION  glutamine--fructose-6-phosphate aminotransferase [isomerizing]
            2-like isoform X8 [Amblyomma americanum].
ACCESSION   XP_077504272
VERSION     XP_077504272.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648146.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..680
                     /product="glutamine--fructose-6-phosphate aminotransferase
                     [isomerizing] 2-like isoform X8"
                     /calculated_mol_wt=76606
     Region          1..680
                     /region_name="PLN02981"
                     /note="glucosamine:fructose-6-phosphate aminotransferase"
                     /db_xref="CDD:215531"
     CDS             1..680
                     /gene="LOC144114414"
                     /coded_by="XM_077648146.1:208..2250"
                     /db_xref="GeneID:144114414"
ORIGIN      
        1 mcgifaylny lepktrreil eclikglqrl eyrgydsagv afdgdaqgnn isiirkigkv
       61 kvledsiwrk ddldmdhehl thigiahtrw athgapsdln shpqrsdpen efvvvhngii
      121 tnykdikqfl iqkgcrfesd tdteviakfi khihnthpdl sfrelveqvi qqlegafalv
      181 fkskkfphqc vatrrgspll vglktksyls tefipvlysk dhrpreshpp sntgglqrta
      241 sttefgppgd dkeieyffas dasaiiehtn rvifledddv aavseghlti hrlkrtlden
      301 vnreiitlkm eiqqimkgnf ssfmqkeife qpesvintmr grlsfeketv vlggikdyit
      361 eikrcrrlll igcgtsyhsa iatrqileel telpvmvela sdfldrntpv frddvcffis
      421 qsgetadtlm alryckqrga livgitntvg ssicreshcg vhinagpeig vastkaytsq
      481 fvslvmfslm mcedrismqp rrteiiqglh rlpdqikevl kldnevkqla qhlyqqksll
      541 vmgrgynhat clegalkike ltymhsegil agelkhgpla lvdkampvmm vltrdpvfpk
      601 cmnalqqvia rdgrpiiice kgdeetkkla fkylevphtv dclqgvltvi plqlmsyhia
      661 vlkgcnvdcp rnlaksvtve
//
LOCUS       XP_077504273             289 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114418 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504273
VERSION     XP_077504273.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648147.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..289
                     /product="uncharacterized protein LOC144114418 isoform X1"
                     /calculated_mol_wt=30311
     CDS             1..289
                     /gene="LOC144114418"
                     /coded_by="XM_077648147.1:291..1160"
                     /db_xref="GeneID:144114418"
ORIGIN      
        1 msinpalkkf riwfvdmgkp seedsatkiq anvgyaksrd gifqivemvl glvaficaes
       61 vaecrgafnc appgclhsyr ffaivsfasv iftgaiflar mlgaahrvpi pakfrplaec
      121 lyllittlly ffadafmiay hgnrlgyqca avlgvaalaa ycvqlallaa eaplgrllpf
      181 rgasaqsapg atkednasql sweppagved adgaapglgp vvlrprrssl dsgggaarrq
      241 dppdsskssr sssrsssdca etasvhsanf vtvnlddvtq spgssalps
//
LOCUS       XP_077504274             286 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114418 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504274
VERSION     XP_077504274.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648148.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..286
                     /product="uncharacterized protein LOC144114418 isoform X2"
                     /calculated_mol_wt=30061
     CDS             1..286
                     /gene="LOC144114418"
                     /coded_by="XM_077648148.1:118..978"
                     /db_xref="GeneID:144114418"
ORIGIN      
        1 mrrrsswfee fanmgkpsee dsatkiqanv gyaksrdgif qivemvlglv aficaesvae
       61 crgafncapp gclhsyrffa ivsfasvift gaiflarmlg aahrvpipak frplaeclyl
      121 littllyffa dafmiayhgn rlgyqcaavl gvaalaaycv qlallaaeap lgrllpfrga
      181 saqsapgatk ednasqlswe ppagvedadg aapglgpvvl rprrssldsg ggaarrqdpp
      241 dsskssrsss rsssdcaeta svhsanfvtv nlddvtqspg ssalps
//
LOCUS       XP_077504275             160 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114419 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504275
VERSION     XP_077504275.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648149.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..160
                     /product="uncharacterized protein LOC144114419 isoform X1"
                     /calculated_mol_wt=17315
     CDS             1..160
                     /gene="LOC144114419"
                     /coded_by="XM_077648149.1:57..539"
                     /db_xref="GeneID:144114419"
ORIGIN      
        1 mdevwsslsp vflisvvtsa lalvftavfa yrtskygfll gknetrlepn avgscvdels
       61 deeneripak pqaakaaesl ieaamteeqk tmererqaat maaaskprss srsgssapgg
      121 gtgraqqkql eeiyrlmaeh sdkfgstsme eivnqmalyr
//
LOCUS       XP_077504276             131 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114419 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504276
VERSION     XP_077504276.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648150.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..131
                     /product="uncharacterized protein LOC144114419 isoform X2"
                     /calculated_mol_wt=14585
     CDS             1..131
                     /gene="LOC144114419"
                     /coded_by="XM_077648150.1:52..447"
                     /db_xref="GeneID:144114419"
ORIGIN      
        1 mdevwsslsp vflisvvtsa lalvftavfa yrtskygfll gknetrlepn avgscvdels
       61 deeneripak pqaakaaesl ieaamteeqk tmereaqqkq leeiyrlmae hsdkfgstsm
      121 eeivnqmaly r
//
LOCUS       XP_077504277             113 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114419 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504277
VERSION     XP_077504277.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648151.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..113
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..113
                     /product="uncharacterized protein LOC144114419 isoform X3"
                     /calculated_mol_wt=12139
     CDS             1..113
                     /gene="LOC144114419"
                     /coded_by="XM_077648151.1:1060..1401"
                     /db_xref="GeneID:144114419"
ORIGIN      
        1 mtvsqgscvd elsdeeneri pakpqaakaa eslieaamte eqktmererq aatmaaaskp
       61 rsssrsgssa pgggtgraqq kqleeiyrlm aehsdkfgst smeeivnqma lyr
//
LOCUS       XP_077504278            1100 aa            linear   INV 25-NOV-2025
DEFINITION  SPT16 homolog, facilitates chromatin remodeling subunit dre4
            isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504278
VERSION     XP_077504278.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648152.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1100
                     /product="SPT16 homolog, facilitates chromatin remodeling
                     subunit dre4 isoform X1"
                     /calculated_mol_wt=124693
     Region          1..1035
                     /region_name="COG5406"
                     /note="Nucleosome binding factor SPN, SPT16 subunit
                     [Transcription, Replication, recombination and repair,
                     Chromatin structure and dynamics]"
                     /db_xref="CDD:227693"
     CDS             1..1100
                     /gene="dre4"
                     /coded_by="XM_077648152.1:58..3360"
                     /db_xref="GeneID:144114420"
ORIGIN      
        1 masltvnkdt fyrrirrlya awkkpdenya slgkmdalvs avgvdeevvy skstaiqtwl
       61 fgyeltdtim vlcesaicfl askkkveflk qlesgkenea qappitllvr dkadkdkanf
      121 eklieaikks rngkvvgefs kdkfpgefmd awrsaisaek fdtvdvsaav ayvmapkeeq
      181 elaltkkacq vtvdvyakyl rdqvmeiida dkkvkhckla egvdqafqek kylggvdpsq
      241 vdscypaiiq sggyynlkfs vvsdkntlhf gaitcalgar yksycsnivr tllvnpsqeq
      301 qdlydfllql eeevvleklr dgvklcdvfa avmarvnkdh keladkmtkn vgfatgiefr
      361 esslviqskn tavarkgmvf ninigfsglq ckgsgdeavk tyalfigdtv lvnegqpati
      421 itnskkklkn iaifikvrkh wwcclfglnl llpqdeeeee eeeeeekegk tkeeepilgr
      481 ggrrtailds klrteqsaee krqqnqklla erlnqaakdr lasqrgvqkd ekirksnvsy
      541 ksvnqmpkep evkelkifvd kkyesvilpi fgipvpyhis tiknisqsve gdytylrinf
      601 fhpgaalgrn egnvfpnpea tflkeityrs tnvkepgeis apssnlntaf rlikevqkkf
      661 ktreaeerek egivkqdtlv lssnkgnpkl kdlyirpniy skrisgilea hsngfrftsv
      721 rgdkvdilyn ntkhaffqpc dgemiillhf tlrnaimfgk kkhndvqfyt evgeittdlg
      781 khqhmhdrdd laaeqaerel rqklktafkt fcekveavtk geiefdtpfr dlgfpgvpyr
      841 stvllqptsg clvnltdwpp fivtleevel vhfervqfhl knfdmvfvfk dyhrkcamvn
      901 avpmnmldhv kewlnscdir ytegiqslnw tkimktitdd pegffesggw sfldpesdge
      961 egdgdddsee ddqynpsded nsgeeesesd eddsedysas evsedeesag gelgsseesg
     1021 kdwseleeea readrnrnef edeytkrkgg kgmdrkrpaq esrssskkks tpspqkngss
     1081 akrrhesdsk gseqrkkhkk
//
LOCUS       XP_077504279            1082 aa            linear   INV 25-NOV-2025
DEFINITION  SPT16 homolog, facilitates chromatin remodeling subunit dre4
            isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504279
VERSION     XP_077504279.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648153.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1082
                     /product="SPT16 homolog, facilitates chromatin remodeling
                     subunit dre4 isoform X2"
                     /calculated_mol_wt=122484
     Region          1..1017
                     /region_name="COG5406"
                     /note="Nucleosome binding factor SPN, SPT16 subunit
                     [Transcription, Replication, recombination and repair,
                     Chromatin structure and dynamics]"
                     /db_xref="CDD:227693"
     CDS             1..1082
                     /gene="dre4"
                     /coded_by="XM_077648153.1:58..3306"
                     /db_xref="GeneID:144114420"
ORIGIN      
        1 masltvnkdt fyrrirrlya awkkpdenya slgkmdalvs avgvdeevvy skstaiqtwl
       61 fgyeltdtim vlcesaicfl askkkveflk qlesgkenea qappitllvr dkadkdkanf
      121 eklieaikks rngkvvgefs kdkfpgefmd awrsaisaek fdtvdvsaav ayvmapkeeq
      181 elaltkkacq vtvdvyakyl rdqvmeiida dkkvkhckla egvdqafqek kylggvdpsq
      241 vdscypaiiq sggyynlkfs vvsdkntlhf gaitcalgar yksycsnivr tllvnpsqeq
      301 qdlydfllql eeevvleklr dgvklcdvfa avmarvnkdh keladkmtkn vgfatgiefr
      361 esslviqskn tavarkgmvf ninigfsglq ckgsgdeavk tyalfigdtv lvnegqpati
      421 itnskkklkn iaifikdeee eeeeeeeeke gktkeeepil grggrrtail dsklrteqsa
      481 eekrqqnqkl laerlnqaak drlasqrgvq kdekirksnv syksvnqmpk epevkelkif
      541 vdkkyesvil pifgipvpyh istiknisqs vegdytylri nffhpgaalg rnegnvfpnp
      601 eatflkeity rstnvkepge isapssnlnt afrlikevqk kfktreaeer ekegivkqdt
      661 lvlssnkgnp klkdlyirpn iyskrisgil eahsngfrft svrgdkvdil ynntkhaffq
      721 pcdgemiill hftlrnaimf gkkkhndvqf ytevgeittd lgkhqhmhdr ddlaaeqaer
      781 elrqklktaf ktfcekveav tkgeiefdtp frdlgfpgvp yrstvllqpt sgclvnltdw
      841 ppfivtleev elvhfervqf hlknfdmvfv fkdyhrkcam vnavpmnmld hvkewlnscd
      901 irytegiqsl nwtkimktit ddpegffesg gwsfldpesd geegdgddds eeddqynpsd
      961 ednsgeeese sdeddsedys asevsedees aggelgssee sgkdwselee eareadrnrn
     1021 efedeytkrk ggkgmdrkrp aqesrssskk kstpspqkng ssakrrhesd skgseqrkkh
     1081 kk
//
LOCUS       XP_077504280             638 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: sodium/hydrogen exchanger 8-like [Amblyomma
            americanum].
ACCESSION   XP_077504280
VERSION     XP_077504280.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648154.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..638
                     /product="LOW QUALITY PROTEIN: sodium/hydrogen exchanger
                     8-like"
                     /calculated_mol_wt=70579
     Region          109..604
                     /region_name="Na_H_Exchanger"
                     /note="Sodium/hydrogen exchanger family; cl01133"
                     /db_xref="CDD:470090"
     CDS             1..638
                     /gene="LOC144114422"
                     /coded_by="XM_077648154.1:134..2050"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:144114422"
ORIGIN      
        1 mfvvnrpray lrlhmepsvm tsakivmtil vllwlapace tasekspqts tqvssirtss
       61 nsssnaaanv tmsisngtds nvstiavtts mlpaqppepv lpqkesaeee hhssmtiffa
      121 lsviavcilm ihfliqanfe flpesvvvvl igaligllmk lissqhlgnw qkeealsptm
      181 fflvllppii yesgynlhkg nffqnigsil ifsivgttis alvigggvyl lgladvaykl
      241 dftesfafgs lisavdpvat laifhaldvd pvlnmlvfge silndavsiv lattilesgs
      301 psmvslttgq lvlhgiqrff lvffcsaaig vafalaaall lkyvdlrknp slefgmmlvf
      361 vyapyglaeg ihlsgimail fngvvmshyt hfnlssvtqi tmqqtlrtis liaetsvfay
      421 lglaifsyrl iikpsliiws ivlcllgraa niyplsflmn yfrehkitkk mmfimwysgl
      481 rgaiayalam hldfddekrh vvvtttliiv lftilviggs tmplmkflka dkqttrkkrr
      541 rarqvtlskt kemgqtidae hlselteeey evnfmksnik gfilldikyl ipfftrrftq
      601 kevqdgkiqm tdltnqwyqa irapidesen edseingv
//
LOCUS       XP_077504281             993 aa            linear   INV 25-NOV-2025
DEFINITION  N-acetylated-alpha-linked acidic dipeptidase 2-like [Amblyomma
            americanum].
ACCESSION   XP_077504281
VERSION     XP_077504281.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648155.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..993
                     /product="N-acetylated-alpha-linked acidic dipeptidase
                     2-like"
                     /calculated_mol_wt=111204
     Region          368..591
                     /region_name="PA"
                     /note="Protease-associated (PA) domain. The PA domain is
                     an insert domain in a diverse fraction of proteases. The
                     significance of the PA domain to many of the proteins in
                     which it is inserted is undetermined. It may be a
                     protein-protein interaction domain. At...; cl28883"
                     /db_xref="CDD:333703"
     Site            500..502
                     /site_type="other"
                     /note="PA/protease or protease-like domain interface
                     [polypeptide binding]"
                     /db_xref="CDD:238300"
     Region          583..835
                     /region_name="Zinc_peptidase_like"
                     /note="Zinc peptidases M18, M20, M28, and M42; cl14876"
                     /db_xref="CDD:472712"
     Region          864..984
                     /region_name="TFR_dimer"
                     /note="Transferrin receptor-like dimerization domain;
                     pfam04253"
                     /db_xref="CDD:461238"
     CDS             1..993
                     /gene="LOC144114423"
                     /coded_by="XM_077648155.1:128..3109"
                     /db_xref="GeneID:144114423"
ORIGIN      
        1 mlpwarrrlv rvhsqimgsq saerlspaas gfheeerpay asrsatsppk qvsydsalag
       61 dcsvmllnve vldednrqpa apppqplplq prysepispd rlerererme rmerlerler
      121 eeeerprwqr sverpalsps agamspiagv asppgqqymr apvspmpmrp qyavspagsr
      181 pripmspmls pqspsrqfvy pgppmarrms gaisplsprs gsrspswpfm tdrqvmaarq
      241 yrkerhnlle wimtwvfvvt lavvllailv fyaswqtrld kkmqehlnll qrlshsprfg
      301 ssvfnelykv dlptevslrt ilktitdlry iagtetespv tiyvdrfmkd nklnhtkift
      361 yvvslshpst tnrnrvqfvd ssgnaiitfs deepsvlgkn lgpqlaysay gragtftati
      421 lygnrcfped ldylaktvgq vlwggallcr ysttespgla vtaaqrkgvq avlffsdphd
      481 vseggasafp qswwmpntai rrssvrvahd igdpsspgys sryaisdlfr apieqtmvpt
      541 lavqpinnkd aaelfrnlqg kecpdkwapa fgvpchlgdt skgklsvsih nvihdsniqn
      601 ilaffpgeve pdryvvfgvp ldswgggava pgsalaqalg lcyiinkqym skkhpwrprr
      661 tivfgawdah efgevgatef vegnrhklsa rtvayfnsdi ctsgqsfsvs gspvfsrafr
      721 eagkwvphfr ntsyynawyt dlsaaagesg qpdlpmlrkt ggfapfvqla gvpsldvafg
      781 nnesrglfyp algtsydvfd ladkfidpgy rvhrmcrell iallwqwsea illpydfkel
      841 strlnqefse istqhkelve rlgirfdsls havklftanl nhfetdltrv dlknplevrr
      901 yndkmmgver sfvqevdnat apsrlrnvvf gqsdtnherm vafpivrdkl erlrmnepdq
      961 deakaaedlr rhislaayal lqatlfiesd gla
//
LOCUS       XP_077504282            2605 aa            linear   INV 25-NOV-2025
DEFINITION  piezo type mechanosensitive ion channel component isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077504282
VERSION     XP_077504282.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648156.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2605
                     /product="piezo type mechanosensitive ion channel
                     component isoform X1"
                     /calculated_mol_wt=293278
     Region          1290..1449
                     /region_name="PIEZO"
                     /note="Piezo; pfam15917"
                     /db_xref="CDD:464941"
     Region          2185..2598
                     /region_name="Piezo_RRas_bdg"
                     /note="Piezo non-specific cation channel, R-Ras-binding
                     domain; pfam12166"
                     /db_xref="CDD:463481"
     CDS             1..2605
                     /gene="Piezo"
                     /coded_by="XM_077648156.1:510..8327"
                     /db_xref="GeneID:144114424"
ORIGIN      
        1 mvkavvcqvl frvalpvvll lavlfrynvf sfvyllllla nplvrgpnsr ntnivrsscy
       61 vkavvaastl fsvaqvvyqi vllstqsysk dhtladscsl qerllalvgl hradgikape
      121 alrlfgidfv vlfaaiftlv icerlatpde tprsgggvrr rrhtflmllg effalllmaa
      181 agilhaslss layfvaflws atwlgmhrpl atgyramrtl lllysamhfv alysyqldym
      241 qevippatlq arllgltslr ipacnstqpa dvdirvlqfr slhwtlyvsp lvvlcfyftv
      301 atvtrlqllq slpyespgmv aaggvgghip rqdskrssqr savsfsrrhr resgavvded
      361 aiqkaplldn dhvgrsyqsm aakdsvepsg sggwsslegt vgsagaspla gapvgagacg
      421 ieddtmagsm pdrspgsrql vllmvlqgcr lltrgsyvvt limmmawsit yhswltfvll
      481 lwscvlwmmp ssrlaclrss palvayaell lllqylysld ltdeelpqkv navnlaqvgl
      541 fkygynsyqp lavkilytim fwitlrqyve hqrmppapgi elrrqmsmga sslgmgtqly
      601 vrrlgrlvqk wltrywiwvv atmlmvislg gtevvlyria ymllflffil vfqvsyqlwi
      661 kvmygfwltv iiysmlvlil iytyqfekfp yywevylhip peiqkdigle vyqsdpgtlf
      721 lklltptffl iitiiqlhyf hqefiklned nyrspssnee eaggverrlq ppvaapaqae
      781 egivaipvev papamtaavl phphsdaees devatpatvv prtkanqpqp pednlspksr
      841 dsawmsleri gsmlsrvaev cwrlleihmm kavllavfil avydvcavhi ifvalvvval
      901 pfrwmqlflt hccsmwasml llskmiyqln fvdrygwatn cssvtgfdgn ttefpapfna
      961 tidnriwigf qkttnltsyc kgyimlivvf sahamvryrq rfyrtrhqlp eprpgvvfie
     1021 anrsnaddgi kqclmyllny ffykfgvevc fvtmvtcigv rldvfalmts lwlcamfllr
     1081 rrnlakvwpf yvaylcfvlp lqylvvvglp pglcieypww dpskkvlret alwlflpdyq
     1141 dpplarkilv dfiqllfgcc qlhvfflens htsiayeggs nkeiydktgr yigqtpnpip
     1201 dfvtytrsyl smvkvflffs fywitlavmf lagtnrvslf amgyvlgcff flwngnefyl
     1261 kpmnvllkmw ntllaynvvv ifikcilqvv gcvfldylsk yscwmvqlfg iaclkrvqne
     1321 plpggstalp nasggtcrvp ldeagllwdg iclafllmqk rlftsyyfhh liieilaqqq
     1381 lasrgaemih eiqmkdvqeq raaerdimek ikrkmdkira nqhkvragey vepethfqai
     1441 rsgdyylfdd fagmdidldl dvksrkpaee edadvkvrgi nallsnamks gtikcaseda
     1501 rlvseeaddl nessrlspiv smdrsplsrt vsgtsqpsks sskplpsssd lpstsadevv
     1561 lpedprpvas tadaapstav vpavpaataa taaaaptaap taapttvatt ipttvpttap
     1621 attsttavaa skpvqleepg qvaagegapq ecqplpaedt yaerirslla fgwalvdsml
     1681 isataklnsv srdyryvark ladekrivka qfvhglattl gepaaksrkf hfhehsaada
     1741 hhpqavvdkk asedmllsgs metgdspdkh dvhkhrpslv rfliacyyai vsrsevvcyl
     1801 vivlnqmksa sllslplplm aflwgslsvp rptktfwiti ityteaivvv kylfqfdffd
     1861 wndsvpvnrp fdpprilgie krkedndyal ydllllllvf fhrfmlkslg lwkdtdrsvs
     1921 vsaiqqqepv sneeqyytaa rrpseaapgl lthvppdqqp gtsgtlelaa lpldvgssgs
     1981 rcyddvlpgq sgldesfvhl sklfdgisry vepfrnffen llhpvyrvtt dvyaymffcd
     2041 finffimvfg ywafgtggse dgvasyfqkn evpvpfliml laqfalivid ralylrkyil
     2101 gklifqvfiv fvihiwmffv lpgisqrsfv eeknlppklw yfikciylil sayqirsgyp
     2161 trilgnffck kynyinyflf kgymlipfly elrslmdwiw tdtsmnltnw lkmedifanv
     2221 fllkcqrrae eeyptprgsr rssltkyglg gvmlfaiilv iwfplllfsl gntvgqtllp
     2281 hdctvelslg gyepifkisa qqgnlrqlpy dswvrlqaey ksnaaaqafl anydaadvav
     2341 vtlngnstai wtvsppsqea liaelrrsav plrlswafsr tvdntnaekv vsnertvqls
     2401 dehvrqslad mlrgtpnnvt vppilprfll vprkgksdvi raldtpgmgp yrnltlrlrt
     2461 gafnnlsars ewwevqefct esypypflre egsctdlslv vfndkvfpqa lsqltgygia
     2521 glyttfvlvv srlirgfmag safsimfddm pnvdrvlqlc ldiylvresr elsleedlfa
     2581 kliflyrspe tlikwtrpad qqpla
//
LOCUS       XP_077504283            2605 aa            linear   INV 25-NOV-2025
DEFINITION  piezo type mechanosensitive ion channel component isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077504283
VERSION     XP_077504283.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648157.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2605
                     /product="piezo type mechanosensitive ion channel
                     component isoform X1"
                     /calculated_mol_wt=293278
     Region          1290..1449
                     /region_name="PIEZO"
                     /note="Piezo; pfam15917"
                     /db_xref="CDD:464941"
     Region          2185..2598
                     /region_name="Piezo_RRas_bdg"
                     /note="Piezo non-specific cation channel, R-Ras-binding
                     domain; pfam12166"
                     /db_xref="CDD:463481"
     CDS             1..2605
                     /gene="Piezo"
                     /coded_by="XM_077648157.1:236..8053"
                     /db_xref="GeneID:144114424"
ORIGIN      
        1 mvkavvcqvl frvalpvvll lavlfrynvf sfvyllllla nplvrgpnsr ntnivrsscy
       61 vkavvaastl fsvaqvvyqi vllstqsysk dhtladscsl qerllalvgl hradgikape
      121 alrlfgidfv vlfaaiftlv icerlatpde tprsgggvrr rrhtflmllg effalllmaa
      181 agilhaslss layfvaflws atwlgmhrpl atgyramrtl lllysamhfv alysyqldym
      241 qevippatlq arllgltslr ipacnstqpa dvdirvlqfr slhwtlyvsp lvvlcfyftv
      301 atvtrlqllq slpyespgmv aaggvgghip rqdskrssqr savsfsrrhr resgavvded
      361 aiqkaplldn dhvgrsyqsm aakdsvepsg sggwsslegt vgsagaspla gapvgagacg
      421 ieddtmagsm pdrspgsrql vllmvlqgcr lltrgsyvvt limmmawsit yhswltfvll
      481 lwscvlwmmp ssrlaclrss palvayaell lllqylysld ltdeelpqkv navnlaqvgl
      541 fkygynsyqp lavkilytim fwitlrqyve hqrmppapgi elrrqmsmga sslgmgtqly
      601 vrrlgrlvqk wltrywiwvv atmlmvislg gtevvlyria ymllflffil vfqvsyqlwi
      661 kvmygfwltv iiysmlvlil iytyqfekfp yywevylhip peiqkdigle vyqsdpgtlf
      721 lklltptffl iitiiqlhyf hqefiklned nyrspssnee eaggverrlq ppvaapaqae
      781 egivaipvev papamtaavl phphsdaees devatpatvv prtkanqpqp pednlspksr
      841 dsawmsleri gsmlsrvaev cwrlleihmm kavllavfil avydvcavhi ifvalvvval
      901 pfrwmqlflt hccsmwasml llskmiyqln fvdrygwatn cssvtgfdgn ttefpapfna
      961 tidnriwigf qkttnltsyc kgyimlivvf sahamvryrq rfyrtrhqlp eprpgvvfie
     1021 anrsnaddgi kqclmyllny ffykfgvevc fvtmvtcigv rldvfalmts lwlcamfllr
     1081 rrnlakvwpf yvaylcfvlp lqylvvvglp pglcieypww dpskkvlret alwlflpdyq
     1141 dpplarkilv dfiqllfgcc qlhvfflens htsiayeggs nkeiydktgr yigqtpnpip
     1201 dfvtytrsyl smvkvflffs fywitlavmf lagtnrvslf amgyvlgcff flwngnefyl
     1261 kpmnvllkmw ntllaynvvv ifikcilqvv gcvfldylsk yscwmvqlfg iaclkrvqne
     1321 plpggstalp nasggtcrvp ldeagllwdg iclafllmqk rlftsyyfhh liieilaqqq
     1381 lasrgaemih eiqmkdvqeq raaerdimek ikrkmdkira nqhkvragey vepethfqai
     1441 rsgdyylfdd fagmdidldl dvksrkpaee edadvkvrgi nallsnamks gtikcaseda
     1501 rlvseeaddl nessrlspiv smdrsplsrt vsgtsqpsks sskplpsssd lpstsadevv
     1561 lpedprpvas tadaapstav vpavpaataa taaaaptaap taapttvatt ipttvpttap
     1621 attsttavaa skpvqleepg qvaagegapq ecqplpaedt yaerirslla fgwalvdsml
     1681 isataklnsv srdyryvark ladekrivka qfvhglattl gepaaksrkf hfhehsaada
     1741 hhpqavvdkk asedmllsgs metgdspdkh dvhkhrpslv rfliacyyai vsrsevvcyl
     1801 vivlnqmksa sllslplplm aflwgslsvp rptktfwiti ityteaivvv kylfqfdffd
     1861 wndsvpvnrp fdpprilgie krkedndyal ydllllllvf fhrfmlkslg lwkdtdrsvs
     1921 vsaiqqqepv sneeqyytaa rrpseaapgl lthvppdqqp gtsgtlelaa lpldvgssgs
     1981 rcyddvlpgq sgldesfvhl sklfdgisry vepfrnffen llhpvyrvtt dvyaymffcd
     2041 finffimvfg ywafgtggse dgvasyfqkn evpvpfliml laqfalivid ralylrkyil
     2101 gklifqvfiv fvihiwmffv lpgisqrsfv eeknlppklw yfikciylil sayqirsgyp
     2161 trilgnffck kynyinyflf kgymlipfly elrslmdwiw tdtsmnltnw lkmedifanv
     2221 fllkcqrrae eeyptprgsr rssltkyglg gvmlfaiilv iwfplllfsl gntvgqtllp
     2281 hdctvelslg gyepifkisa qqgnlrqlpy dswvrlqaey ksnaaaqafl anydaadvav
     2341 vtlngnstai wtvsppsqea liaelrrsav plrlswafsr tvdntnaekv vsnertvqls
     2401 dehvrqslad mlrgtpnnvt vppilprfll vprkgksdvi raldtpgmgp yrnltlrlrt
     2461 gafnnlsars ewwevqefct esypypflre egsctdlslv vfndkvfpqa lsqltgygia
     2521 glyttfvlvv srlirgfmag safsimfddm pnvdrvlqlc ldiylvresr elsleedlfa
     2581 kliflyrspe tlikwtrpad qqpla
//
LOCUS       XP_077504284            2593 aa            linear   INV 25-NOV-2025
DEFINITION  piezo type mechanosensitive ion channel component isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077504284
VERSION     XP_077504284.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648158.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2593
                     /product="piezo type mechanosensitive ion channel
                     component isoform X2"
                     /calculated_mol_wt=292041
     Region          1278..1437
                     /region_name="PIEZO"
                     /note="Piezo; pfam15917"
                     /db_xref="CDD:464941"
     Region          2173..2586
                     /region_name="Piezo_RRas_bdg"
                     /note="Piezo non-specific cation channel, R-Ras-binding
                     domain; pfam12166"
                     /db_xref="CDD:463481"
     CDS             1..2593
                     /gene="Piezo"
                     /coded_by="XM_077648158.1:537..8318"
                     /db_xref="GeneID:144114424"
ORIGIN      
        1 mvkavvcqvl frvalpvvll lavlfrynvf sfvyllllla nplvrgpnsr ntnivrsscy
       61 vkavvaastl fsvaqvvyqi vllstqsysk dhtladscsl qerllalvgl hradgikape
      121 alrlfgidfv vlfaaiftlv icerlatpde tprsgggvrr rrhtflmllg effalllmaa
      181 agilhaslss layfvaflws atwlgmhrpl atgyramrtl lllysamhfv alysyqldym
      241 qevippatlq arllgltslr ipacnstqpa dvdirvlqfr slhwtlyvsp lvvlcfyftv
      301 atvtrlqllq slpyespgmv aaggvgghip rqdskrssqr savsfsrrhr resglldndh
      361 vgrsyqsmaa kdsvepsgsg gwsslegtvg sagasplaga pvgagacgie ddtmagsmpd
      421 rspgsrqlvl lmvlqgcrll trgsyvvtli mmmawsityh swltfvlllw scvlwmmpss
      481 rlaclrsspa lvayaellll lqylysldlt deelpqkvna vnlaqvglfk ygynsyqpla
      541 vkilytimfw itlrqyvehq rmppapgiel rrqmsmgass lgmgtqlyvr rlgrlvqkwl
      601 trywiwvvat mlmvislggt evvlyriaym llflffilvf qvsyqlwikv mygfwltvii
      661 ysmlvliliy tyqfekfpyy wevylhippe iqkdiglevy qsdpgtlflk lltptfflii
      721 tiiqlhyfhq efiklnedny rspssneeea ggverrlqpp vaapaqaeeg ivaipvevpa
      781 pamtaavlph phsdaeesde vatpatvvpr tkanqpqppe dnlspksrds awmslerigs
      841 mlsrvaevcw rlleihmmka vllavfilav ydvcavhiif valvvvalpf rwmqlflthc
      901 csmwasmlll skmiyqlnfv drygwatncs svtgfdgntt efpapfnati dnriwigfqk
      961 ttnltsyckg yimlivvfsa hamvryrqrf yrtrhqlpep rpgvvfiean rsnaddgikq
     1021 clmyllnyff ykfgvevcfv tmvtcigvrl dvfalmtslw lcamfllrrr nlakvwpfyv
     1081 aylcfvlplq ylvvvglppg lcieypwwdp skkvlretal wlflpdyqdp plarkilvdf
     1141 iqllfgccql hvfflensht siayeggsnk eiydktgryi gqtpnpipdf vtytrsylsm
     1201 vkvflffsfy witlavmfla gtnrvslfam gyvlgcfffl wngnefylkp mnvllkmwnt
     1261 llaynvvvif ikcilqvvgc vfldylskys cwmvqlfgia clkrvqnepl pggstalpna
     1321 sggtcrvpld eagllwdgic lafllmqkrl ftsyyfhhli ieilaqqqla srgaemihei
     1381 qmkdvqeqra aerdimekik rkmdkiranq hkvrageyve pethfqairs gdyylfddfa
     1441 gmdidldldv ksrkpaeeed advkvrgina llsnamksgt ikcasedarl vseeaddlne
     1501 ssrlspivsm drsplsrtvs gtsqpsksss kplpsssdlp stsadevvlp edprpvasta
     1561 daapstavvp avpaataata aaaptaapta apttvattip ttvpttapat tsttavaask
     1621 pvqleepgqv aagegapqec qplpaedtya erirsllafg walvdsmlis ataklnsvsr
     1681 dyryvarkla dekrivkaqf vhglattlge paaksrkfhf hehsaadahh pqavvdkkas
     1741 edmllsgsme tgdspdkhdv hkhrpslvrf liacyyaivs rsevvcylvi vlnqmksasl
     1801 lslplplmaf lwgslsvprp tktfwitiit yteaivvvky lfqfdffdwn dsvpvnrpfd
     1861 pprilgiekr kedndyalyd llllllvffh rfmlkslglw kdtdrsvsvs aiqqqepvsn
     1921 eeqyytaarr pseaapgllt hvppdqqpgt sgtlelaalp ldvgssgsrc yddvlpgqsg
     1981 ldesfvhlsk lfdgisryve pfrnffenll hpvyrvttdv yaymffcdfi nffimvfgyw
     2041 afgtggsedg vasyfqknev pvpflimlla qfalividra lylrkyilgk lifqvfivfv
     2101 ihiwmffvlp gisqrsfvee knlppklwyf ikciylilsa yqirsgyptr ilgnffckky
     2161 nyinyflfkg ymlipflyel rslmdwiwtd tsmnltnwlk medifanvfl lkcqrraeee
     2221 yptprgsrrs sltkyglggv mlfaiilviw fplllfslgn tvgqtllphd ctvelslggy
     2281 epifkisaqq gnlrqlpyds wvrlqaeyks naaaqaflan ydaadvavvt lngnstaiwt
     2341 vsppsqeali aelrrsavpl rlswafsrtv dntnaekvvs nertvqlsde hvrqsladml
     2401 rgtpnnvtvp pilprfllvp rkgksdvira ldtpgmgpyr nltlrlrtga fnnlsarsew
     2461 wevqefctes ypypflreeg sctdlslvvf ndkvfpqals qltgygiagl yttfvlvvsr
     2521 lirgfmagsa fsimfddmpn vdrvlqlcld iylvresrel sleedlfakl iflyrspetl
     2581 ikwtrpadqq pla
//
LOCUS       XP_077504285             183 aa            linear   INV 25-NOV-2025
DEFINITION  density-regulated protein [Amblyomma americanum].
ACCESSION   XP_077504285
VERSION     XP_077504285.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648159.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="density-regulated protein"
                     /calculated_mol_wt=20356
     Region          20..178
                     /region_name="eIF1_SUI1_like"
                     /note="Eukaryotic initiation factor 1 and related
                     proteins; cl00229"
                     /db_xref="CDD:469673"
     Site            order(107,110..111,113,131,134..136,138..140,142,152,155)
                     /site_type="other"
                     /note="putative rRNA binding site [nucleotide binding]"
                     /db_xref="CDD:211320"
     CDS             1..183
                     /gene="DENR"
                     /coded_by="XM_077648159.1:212..763"
                     /db_xref="GeneID:144114425"
ORIGIN      
        1 mseggteglp tgplpgvsyp ieviycgecs mpleyceyyp dsqkckewlq knlpeeferr
       61 mnlsdapqtg aggdeekkrq krggkgmvka kkrvekdkri clsrasrgkk kfvtvvtgls
      121 tfdidlkdas kffshkfscg ssvtgddeiv iqgdvkddvf dvisekwpei dedliedlgd
      181 qsr
//
LOCUS       XP_077504286             183 aa            linear   INV 25-NOV-2025
DEFINITION  density-regulated protein [Amblyomma americanum].
ACCESSION   XP_077504286
VERSION     XP_077504286.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648160.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="density-regulated protein"
                     /calculated_mol_wt=20356
     Region          20..178
                     /region_name="eIF1_SUI1_like"
                     /note="Eukaryotic initiation factor 1 and related
                     proteins; cl00229"
                     /db_xref="CDD:469673"
     Site            order(107,110..111,113,131,134..136,138..140,142,152,155)
                     /site_type="other"
                     /note="putative rRNA binding site [nucleotide binding]"
                     /db_xref="CDD:211320"
     CDS             1..183
                     /gene="DENR"
                     /coded_by="XM_077648160.1:138..689"
                     /db_xref="GeneID:144114425"
ORIGIN      
        1 mseggteglp tgplpgvsyp ieviycgecs mpleyceyyp dsqkckewlq knlpeeferr
       61 mnlsdapqtg aggdeekkrq krggkgmvka kkrvekdkri clsrasrgkk kfvtvvtgls
      121 tfdidlkdas kffshkfscg ssvtgddeiv iqgdvkddvf dvisekwpei dedliedlgd
      181 qsr
//
LOCUS       XP_077504287             183 aa            linear   INV 25-NOV-2025
DEFINITION  density-regulated protein [Amblyomma americanum].
ACCESSION   XP_077504287
VERSION     XP_077504287.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648161.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="density-regulated protein"
                     /calculated_mol_wt=20356
     Region          20..178
                     /region_name="eIF1_SUI1_like"
                     /note="Eukaryotic initiation factor 1 and related
                     proteins; cl00229"
                     /db_xref="CDD:469673"
     Site            order(107,110..111,113,131,134..136,138..140,142,152,155)
                     /site_type="other"
                     /note="putative rRNA binding site [nucleotide binding]"
                     /db_xref="CDD:211320"
     CDS             1..183
                     /gene="DENR"
                     /coded_by="XM_077648161.1:262..813"
                     /db_xref="GeneID:144114425"
ORIGIN      
        1 mseggteglp tgplpgvsyp ieviycgecs mpleyceyyp dsqkckewlq knlpeeferr
       61 mnlsdapqtg aggdeekkrq krggkgmvka kkrvekdkri clsrasrgkk kfvtvvtgls
      121 tfdidlkdas kffshkfscg ssvtgddeiv iqgdvkddvf dvisekwpei dedliedlgd
      181 qsr
//
LOCUS       XP_077504288             183 aa            linear   INV 25-NOV-2025
DEFINITION  density-regulated protein [Amblyomma americanum].
ACCESSION   XP_077504288
VERSION     XP_077504288.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648162.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="density-regulated protein"
                     /calculated_mol_wt=20356
     Region          20..178
                     /region_name="eIF1_SUI1_like"
                     /note="Eukaryotic initiation factor 1 and related
                     proteins; cl00229"
                     /db_xref="CDD:469673"
     Site            order(107,110..111,113,131,134..136,138..140,142,152,155)
                     /site_type="other"
                     /note="putative rRNA binding site [nucleotide binding]"
                     /db_xref="CDD:211320"
     CDS             1..183
                     /gene="DENR"
                     /coded_by="XM_077648162.1:143..694"
                     /db_xref="GeneID:144114425"
ORIGIN      
        1 mseggteglp tgplpgvsyp ieviycgecs mpleyceyyp dsqkckewlq knlpeeferr
       61 mnlsdapqtg aggdeekkrq krggkgmvka kkrvekdkri clsrasrgkk kfvtvvtgls
      121 tfdidlkdas kffshkfscg ssvtgddeiv iqgdvkddvf dvisekwpei dedliedlgd
      181 qsr
//
LOCUS       XP_077504289             488 aa            linear   INV 25-NOV-2025
DEFINITION  syntrophin-like 1 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504289
VERSION     XP_077504289.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648163.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..488
                     /product="syntrophin-like 1 isoform X1"
                     /calculated_mol_wt=54019
     Region          70..152
                     /region_name="PDZ_syntrophin-like"
                     /note="PDZ domain of syntrophins, and related domains;
                     cd06801"
                     /db_xref="CDD:467262"
     Site            order(81..87,99,102,132,136,139..140)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467262"
     Region          <196..250
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          273..382
                     /region_name="PH"
                     /note="PH domain; pfam00169"
                     /db_xref="CDD:459697"
     CDS             1..488
                     /gene="Syn1"
                     /coded_by="XM_077648163.1:132..1598"
                     /db_xref="GeneID:144114426"
ORIGIN      
        1 matttssytr tgvlevyakq qwhkvlatle edhlslclde syevppitnn gtlsdsepsd
       61 ipesiantkr vvrvvkqdnn glgisikggk enkmpilisk ifkgmaadtt eqlyvgdail
      121 svngedlrda thdeavralk ragkvvdlev kylrevtpyf rkasvlaemg wdfapgglls
      181 rgaaasshpq grpdcrrvpl llcqlarslt vpeprvlelh spdrrsvcll rcadaaqcsa
      241 wfnalhaala rvmaqavvea ghllrdvldq aqlqhmgwls ektredsvta qwravfvait
      301 drdllfydlv pwtkeawavp vhsvpllhtr lvttssgras vgpsaggeat tltlrlgtrq
      361 gvqsrlmrve thrdlavwar hlvhgahlav aaakevrfac lfqgqectlt lhyedgfllq
      421 enrrqltlwq fpferlrnsg ddgmrlawld fggedgdkel dlmqcpkpfv ftlhtflsak
      481 vtrmglva
//
LOCUS       XP_077504290             480 aa            linear   INV 25-NOV-2025
DEFINITION  syntrophin-like 1 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504290
VERSION     XP_077504290.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648164.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..480
                     /product="syntrophin-like 1 isoform X2"
                     /calculated_mol_wt=53217
     Region          70..152
                     /region_name="PDZ_syntrophin-like"
                     /note="PDZ domain of syntrophins, and related domains;
                     cd06801"
                     /db_xref="CDD:467262"
     Site            order(81..87,99,102,132,136,139..140)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467262"
     Region          <196..250
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          273..374
                     /region_name="PH"
                     /note="PH domain; pfam00169"
                     /db_xref="CDD:459697"
     CDS             1..480
                     /gene="Syn1"
                     /coded_by="XM_077648164.1:133..1575"
                     /db_xref="GeneID:144114426"
ORIGIN      
        1 matttssytr tgvlevyakq qwhkvlatle edhlslclde syevppitnn gtlsdsepsd
       61 ipesiantkr vvrvvkqdnn glgisikggk enkmpilisk ifkgmaadtt eqlyvgdail
      121 svngedlrda thdeavralk ragkvvdlev kylrevtpyf rkasvlaemg wdfapgglls
      181 rgaaasshpq grpdcrrvpl llcqlarslt vpeprvlelh spdrrsvcll rcadaaqcsa
      241 wfnalhaala rvmaqavvea ghllrdvldq aqlqhmgwls ektredsvta qwravfvait
      301 drdllfydlv pwtkeawavp vhsvpllhtr asvgpsagge attltlrlgt rqgvqsrlmr
      361 vethrdlavw arhlvhgahl avaaakevrf aclfqgqect ltlhyedgfl lqenrrqltl
      421 wqfpferlrn sgddgmrlaw ldfggedgdk eldlmqcpkp fvftlhtfls akvtrmglva
//
LOCUS       XP_077504291             223 aa            linear   INV 25-NOV-2025
DEFINITION  Fanconi anemia core complex-associated protein 24-like [Amblyomma
            americanum].
ACCESSION   XP_077504291
VERSION     XP_077504291.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648165.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..223
                     /product="Fanconi anemia core complex-associated protein
                     24-like"
                     /calculated_mol_wt=24205
     Region          26..147
                     /region_name="XPF_nuclease_FAAP24"
                     /note="XPF-like nuclease domain of Fanconi anemia
                     associated protein 24 kDa (FAAP24); cd20076"
                     /db_xref="CDD:410852"
     Site            order(111..112,115..116,119..120,125..128,134,138,
                     141..142,144..145)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:410852"
     Region          <157..221
                     /region_name="UvrC"
                     /note="Excinuclease UvrABC, nuclease subunit [Replication,
                     recombination and repair]; COG0322"
                     /db_xref="CDD:440091"
     CDS             1..223
                     /gene="LOC144114428"
                     /coded_by="XM_077648165.1:163..834"
                     /db_xref="GeneID:144114428"
ORIGIN      
        1 mntsirgqis qptcdairkd vrvpvghvvi aekwagselh ialksllqtv vsdhlglidf
       61 ypssnsavly iseaellnen avkrkivklr knnakrpyvi aeqaaalqeq ynnlqqfavm
      121 elgipliavh nqteaaqlla qmeavesgek qnpflvqrrv apmgssvttc llrvpglgev
      181 karallqkyp slralslctv ddltkvvgpa saasvhrffn ggk
//
LOCUS       XP_077504292             249 aa            linear   INV 25-NOV-2025
DEFINITION  (3R)-3-hydroxyacyl-CoA dehydrogenase-like [Amblyomma americanum].
ACCESSION   XP_077504292
VERSION     XP_077504292.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648166.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..249
                     /product="(3R)-3-hydroxyacyl-CoA dehydrogenase-like"
                     /calculated_mol_wt=25277
     Region          2..246
                     /region_name="FabG"
                     /note="NAD(P)-dependent dehydrogenase, short-chain alcohol
                     dehydrogenase family [Lipid transport and metabolism];
                     COG1028"
                     /db_xref="CDD:440651"
     CDS             1..249
                     /gene="LOC144114429"
                     /coded_by="XM_077648166.1:157..906"
                     /db_xref="GeneID:144114429"
ORIGIN      
        1 msltgrlaav tggasgigka vcfalarega avvvgdinfd gaveiatalp gvghphqafk
       61 vdvssgesva affaaitktc tlplsvavsc agilkpaalv dmseeffdev mgvnfkgtfl
      121 fcrdavramq aarvsdgtlv tvasnwarsg sprysayaas kaamvaftks lalelaaqav
      181 rcncvlpsyt dtpmtagnsa qdkardaaks alnraarpee vadvvaflcg prssyvngsa
      241 idinggavv
//
LOCUS       XP_077504293            2091 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: VIR_N domain-containing protein [Amblyomma
            americanum].
ACCESSION   XP_077504293
VERSION     XP_077504293.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648167.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 2 indels
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2091
                     /product="LOW QUALITY PROTEIN: VIR_N domain-containing
                     protein"
                     /calculated_mol_wt=229412
     Region          3..253
                     /region_name="VIR_N"
                     /note="Virilizer, N-terminal; pfam15912"
                     /db_xref="CDD:464938"
     CDS             1..2091
                     /gene="vir"
                     /coded_by="XM_077648167.1:90..6365"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 2 bases in 2 codons"
                     /db_xref="GeneID:144114430"
ORIGIN      
        1 mtdacellff dtfshesnee tnldlvqfpk pvyiheirii plgtrvqadf pgghrlgatn
       61 psefqveffv ndlskrnast feklgsfeyk qnaeiqfmtk kiptdglvlr gwysaitlav
      121 ygiinkvvre ppsppppppp qaslptkmke gipkskwklc tmvaswldsv nnteeppqrp
      181 pdfergsess iplvplewsa pgpaatasgl ssdhpeggrh aipygdprap gygskswaaq
      241 nsqeasqksw egsgsshsqq kaaqslqqsk gsqqtsrfls ppdskdrytp ppikresdha
      301 asaetsspgr essgsggkrr dsthqkehed prrrsssegi irvidprrps tpplprpasp
      361 pihsprprtp pktppmtppr spcatqspde gddeldddre vtprgprtpk eeepdepesp
      421 akpssvkqaq trpaaprtpp iitnaapipt iqtvsavppa atvipmepig dglledddly
      481 esitpdsspe rfpgdfpeea dmtpvanmdd ddngfeaiss edepylsdgd ndigmvdvdy
      541 dvgddswnyl spfdpfqcel splqyfkdps ltpyevekar rgntpaeise ealkvfdsls
      601 aftdrehsdk wveamesaav ylsadalvel fenkefeaaa nalldwveeg lslkaalnqp
      661 qpaykvrhmk agirlatvlf ltgdgvvakl ldrgvpekll elfmapymsl plklqivral
      721 daatygragm qvlftrkmna pxeahvdvgl dsiaedlnef yqskmvepmt gyqillkllf
      781 lkqstratva ltallkklhl ydlvckxhvt vdkiaqsskl cevkekdend eeqtrleldt
      841 egciqhdcse elekaaisld llcrsyrkar kemaqplryl paqsrfeapq tpfdpypalf
      901 kmfkenhlle mlcvlasspl ssshtgitsa lrellaellk tcpgmlflls dheaanclhr
      961 allqeesgmv gledtvchqv gihlvyhlqa lkhvdsllaf htrgnlkkel ddadvvsclh
     1021 dlytiifsdv graavvdvla mdqnldalip flkftgdrdf dmrlcksvct gyatelvllt
     1081 vhnsdsaqll qnyseilhgl sqqelpqrlq elaawmeptl klpsyshesv gylmgcvkaa
     1141 vdsaaslppv litslrilqy lglpprefrs gesddeeelk ykyvimqifs qnglssflai
     1201 lqkiseeflk pwhgsaslvg qqgalvvavl kpvvnllqfm lgyliscrgt efkdvtavpv
     1261 llhtymllgm vppsspahsl aqkvsvevie alltytqpql spteteealn kslwtqmise
     1321 llkftmrgpc tfltgltvfs ellplplplp ireplraedl tkvvncrklw sahlhslsad
     1381 iqeilgtlgy stcstvqqll rrvcvqlsdl aapsasvvsr amldamhssl vspcfgtslg
     1441 geirpltpqn wpptggtlsl ldllsflvth apfkvavlqa iksggkygdi fnymltlfnl
     1501 psdepthiqi qecilatiqa lcdteialah vedappgstn mapepsrtng dekpdvsqal
     1561 krlvnalpsk eqlnticssl vqhltnsshs ystvvlalri limltehdfg fyavktaldk
     1621 eelslwflfe rlstsfskds sdflstlsas vqllrtlssv egvvslgqlr tlhlsaadma
     1681 ahlawerrde keshpllrle kmlmdysgee ealesllngi tnqvellgka astpekeiae
     1741 pvlpaqdgls aqfasrtvyv laevderrls ptywlsavle daeeepdqqv evdlvslmer
     1801 tlpdfnlkse leklvlpgne gvditksplg skrkllmgpd gsepstkkpf iapmrgrgls
     1861 rgslghqsra ndpfrsrppn tsrppsmhvd dflaleshqh plgtgvakrp lvkevvgvhg
     1921 cgrgdtagga mfdvtrpgps rsssarffsp pslygrrels rmdgrdfgsp pfglgsarss
     1981 smvpraiptw serttvpksm lvssnvvalt agpsdsqsfg slidvrsssr srgvdpygmt
     2041 lssmgssggs ltgdrflrgi rgtmmsghap thwmdprtka pdsraswdsl k
//
LOCUS       XP_077504294             434 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylinositol glycan anchor biosynthesis class U [Amblyomma
            americanum].
ACCESSION   XP_077504294
VERSION     XP_077504294.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648168.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..434
                     /product="phosphatidylinositol glycan anchor biosynthesis
                     class U"
                     /calculated_mol_wt=49592
     Region          11..394
                     /region_name="PIG-U"
                     /note="GPI transamidase subunit PIG-U; pfam06728"
                     /db_xref="CDD:429085"
     CDS             1..434
                     /gene="PIG-U"
                     /coded_by="XM_077648168.1:29..1333"
                     /db_xref="GeneID:144114432"
ORIGIN      
        1 maliscglil vlgfvlrlgl iytpvakrvs drvevstpmn swrrvtegly lhrmqlspyd
       61 gdifheppls lmvydyftka fdeqylpllf iacdlltall lalgakkcgs ymaeqetqvk
      121 kaksaehigl tleavnaipk lvltvymlsp ysllncigma ttlfsnllma lffasfvhgw
      181 wliasvvlai etyksfypvm lllptlfylf evkkkassqr wakfvvsavv vtflatlsgl
      241 lyrsylftgs wqflestygc icavpdltpn igvfwyffte vfehfrvffl wifqlnafvy
      301 mvplgirlrk eplllfflll alaavfksyp sigdialyts llpiwrhvfp ymkqyflvgc
      361 ifvscsalap llwhlwifss sananfyfgi tlafntgqif litdllfayv krkfylengd
      421 pkelqeknlk ielr
//
LOCUS       XP_077504295             201 aa            linear   INV 25-NOV-2025
DEFINITION  MARVEL domain-containing protein 1-like [Amblyomma americanum].
ACCESSION   XP_077504295
VERSION     XP_077504295.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648169.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..201
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..201
                     /product="MARVEL domain-containing protein 1-like"
                     /calculated_mol_wt=21841
     Region          50..179
                     /region_name="MARVEL"
                     /note="Membrane-associating domain; pfam01284"
                     /db_xref="CDD:366555"
     CDS             1..201
                     /gene="LOC144114433"
                     /coded_by="XM_077648169.1:209..814"
                     /db_xref="GeneID:144114433"
ORIGIN      
        1 mlqemtrdae mnnqntqkte tntsrsarvt atvkveplpv geglrlnpyy fktvpgilkl
       61 iqvlfgmicl gcaspaligy arfflfvaal cyvvtvllci iyvlgvntal salpwllgel
      121 aytvtavvly iiasvtqlvs qsgyyetwpv mfsalngqyi aagvfgllqa ivygagafll
      181 fqdwkatrpp ppppqpprpt n
//
LOCUS       XP_077504296             201 aa            linear   INV 25-NOV-2025
DEFINITION  MARVEL domain-containing protein 1-like [Amblyomma americanum].
ACCESSION   XP_077504296
VERSION     XP_077504296.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648170.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..201
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..201
                     /product="MARVEL domain-containing protein 1-like"
                     /calculated_mol_wt=21841
     Region          50..179
                     /region_name="MARVEL"
                     /note="Membrane-associating domain; pfam01284"
                     /db_xref="CDD:366555"
     CDS             1..201
                     /gene="LOC144114433"
                     /coded_by="XM_077648170.1:648..1253"
                     /db_xref="GeneID:144114433"
ORIGIN      
        1 mlqemtrdae mnnqntqkte tntsrsarvt atvkveplpv geglrlnpyy fktvpgilkl
       61 iqvlfgmicl gcaspaligy arfflfvaal cyvvtvllci iyvlgvntal salpwllgel
      121 aytvtavvly iiasvtqlvs qsgyyetwpv mfsalngqyi aagvfgllqa ivygagafll
      181 fqdwkatrpp ppppqpprpt n
//
LOCUS       XP_077504297             605 aa            linear   INV 25-NOV-2025
DEFINITION  kelch-like protein 36 [Amblyomma americanum].
ACCESSION   XP_077504297
VERSION     XP_077504297.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648171.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..605
                     /product="kelch-like protein 36"
                     /calculated_mol_wt=66817
     Region          25..145
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          45..576
                     /region_name="PHA03098"
                     /note="kelch-like protein; Provisional"
                     /db_xref="CDD:222983"
     Region          321..368
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          372..423
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          427..471
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          474..525
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          527..577
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     CDS             1..605
                     /gene="LOC144114434"
                     /coded_by="XM_077648171.1:584..2401"
                     /db_xref="GeneID:144114434"
ORIGIN      
        1 mkevsrhsdg tvpgfrmvhf espphasall nglnllrarg qlldvtliag grefkahrvv
       61 laacsdyfra mftdamlesr qpeiclngvs aqglrclley aytsrlvlsl aniqdvlata
      121 nhvgltpvve acssylqdql dlencidvat laetyslrrl rkrvyrfica nlhqfaktpe
      181 fqrlsttqme hllacdfpvn cpegdvlsfv lswvacdvag rlsqarklvs yvcfseipas
      241 klaelwdspv lqclfsrrip phlsgiggqp ppslvntrgm elvllkvggf glsgvtneit
      301 yylpsaarwk yltsiphveq cnfgtavlgn elyvvggcfn qslhqenvhp fgfrynalig
      361 ewstmapmrw ercrfalcva rdhlyavgga gevlgdtdtp edgeahcery dphsdvwipv
      421 aplpgartqh agtawgpylf vsgglnadsv lnsllrydtr tdswetmapm stpradhsmv
      481 vysdrlvvcg gwyedgatgt rvlaesveay diatnawtpv ttvptpryha gvavlgswly
      541 tvggfhsdtt fdrasgiver fdldgnlgwe eaepypqdvw ehvcctlfvp rcrddldvis
      601 dktlm
//
LOCUS       XP_077504298             605 aa            linear   INV 25-NOV-2025
DEFINITION  kelch-like protein 36 [Amblyomma americanum].
ACCESSION   XP_077504298
VERSION     XP_077504298.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648172.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..605
                     /product="kelch-like protein 36"
                     /calculated_mol_wt=66817
     Region          25..145
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          45..576
                     /region_name="PHA03098"
                     /note="kelch-like protein; Provisional"
                     /db_xref="CDD:222983"
     Region          321..368
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          372..423
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          427..471
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          474..525
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          527..577
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     CDS             1..605
                     /gene="LOC144114434"
                     /coded_by="XM_077648172.1:339..2156"
                     /db_xref="GeneID:144114434"
ORIGIN      
        1 mkevsrhsdg tvpgfrmvhf espphasall nglnllrarg qlldvtliag grefkahrvv
       61 laacsdyfra mftdamlesr qpeiclngvs aqglrclley aytsrlvlsl aniqdvlata
      121 nhvgltpvve acssylqdql dlencidvat laetyslrrl rkrvyrfica nlhqfaktpe
      181 fqrlsttqme hllacdfpvn cpegdvlsfv lswvacdvag rlsqarklvs yvcfseipas
      241 klaelwdspv lqclfsrrip phlsgiggqp ppslvntrgm elvllkvggf glsgvtneit
      301 yylpsaarwk yltsiphveq cnfgtavlgn elyvvggcfn qslhqenvhp fgfrynalig
      361 ewstmapmrw ercrfalcva rdhlyavgga gevlgdtdtp edgeahcery dphsdvwipv
      421 aplpgartqh agtawgpylf vsgglnadsv lnsllrydtr tdswetmapm stpradhsmv
      481 vysdrlvvcg gwyedgatgt rvlaesveay diatnawtpv ttvptpryha gvavlgswly
      541 tvggfhsdtt fdrasgiver fdldgnlgwe eaepypqdvw ehvcctlfvp rcrddldvis
      601 dktlm
//
LOCUS       XP_077504299             605 aa            linear   INV 25-NOV-2025
DEFINITION  kelch-like protein 36 [Amblyomma americanum].
ACCESSION   XP_077504299
VERSION     XP_077504299.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648173.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..605
                     /product="kelch-like protein 36"
                     /calculated_mol_wt=66817
     Region          25..145
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          45..576
                     /region_name="PHA03098"
                     /note="kelch-like protein; Provisional"
                     /db_xref="CDD:222983"
     Region          321..368
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          372..423
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          427..471
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          474..525
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          527..577
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     CDS             1..605
                     /gene="LOC144114434"
                     /coded_by="XM_077648173.1:452..2269"
                     /db_xref="GeneID:144114434"
ORIGIN      
        1 mkevsrhsdg tvpgfrmvhf espphasall nglnllrarg qlldvtliag grefkahrvv
       61 laacsdyfra mftdamlesr qpeiclngvs aqglrclley aytsrlvlsl aniqdvlata
      121 nhvgltpvve acssylqdql dlencidvat laetyslrrl rkrvyrfica nlhqfaktpe
      181 fqrlsttqme hllacdfpvn cpegdvlsfv lswvacdvag rlsqarklvs yvcfseipas
      241 klaelwdspv lqclfsrrip phlsgiggqp ppslvntrgm elvllkvggf glsgvtneit
      301 yylpsaarwk yltsiphveq cnfgtavlgn elyvvggcfn qslhqenvhp fgfrynalig
      361 ewstmapmrw ercrfalcva rdhlyavgga gevlgdtdtp edgeahcery dphsdvwipv
      421 aplpgartqh agtawgpylf vsgglnadsv lnsllrydtr tdswetmapm stpradhsmv
      481 vysdrlvvcg gwyedgatgt rvlaesveay diatnawtpv ttvptpryha gvavlgswly
      541 tvggfhsdtt fdrasgiver fdldgnlgwe eaepypqdvw ehvcctlfvp rcrddldvis
      601 dktlm
//
LOCUS       XP_077504300             605 aa            linear   INV 25-NOV-2025
DEFINITION  kelch-like protein 36 [Amblyomma americanum].
ACCESSION   XP_077504300
VERSION     XP_077504300.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648174.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..605
                     /product="kelch-like protein 36"
                     /calculated_mol_wt=66817
     Region          25..145
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          45..576
                     /region_name="PHA03098"
                     /note="kelch-like protein; Provisional"
                     /db_xref="CDD:222983"
     Region          321..368
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          372..423
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          427..471
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          474..525
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          527..577
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     CDS             1..605
                     /gene="LOC144114434"
                     /coded_by="XM_077648174.1:142..1959"
                     /db_xref="GeneID:144114434"
ORIGIN      
        1 mkevsrhsdg tvpgfrmvhf espphasall nglnllrarg qlldvtliag grefkahrvv
       61 laacsdyfra mftdamlesr qpeiclngvs aqglrclley aytsrlvlsl aniqdvlata
      121 nhvgltpvve acssylqdql dlencidvat laetyslrrl rkrvyrfica nlhqfaktpe
      181 fqrlsttqme hllacdfpvn cpegdvlsfv lswvacdvag rlsqarklvs yvcfseipas
      241 klaelwdspv lqclfsrrip phlsgiggqp ppslvntrgm elvllkvggf glsgvtneit
      301 yylpsaarwk yltsiphveq cnfgtavlgn elyvvggcfn qslhqenvhp fgfrynalig
      361 ewstmapmrw ercrfalcva rdhlyavgga gevlgdtdtp edgeahcery dphsdvwipv
      421 aplpgartqh agtawgpylf vsgglnadsv lnsllrydtr tdswetmapm stpradhsmv
      481 vysdrlvvcg gwyedgatgt rvlaesveay diatnawtpv ttvptpryha gvavlgswly
      541 tvggfhsdtt fdrasgiver fdldgnlgwe eaepypqdvw ehvcctlfvp rcrddldvis
      601 dktlm
//
LOCUS       XP_077504301             605 aa            linear   INV 25-NOV-2025
DEFINITION  kelch-like protein 36 [Amblyomma americanum].
ACCESSION   XP_077504301
VERSION     XP_077504301.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648175.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..605
                     /product="kelch-like protein 36"
                     /calculated_mol_wt=66817
     Region          25..145
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          45..576
                     /region_name="PHA03098"
                     /note="kelch-like protein; Provisional"
                     /db_xref="CDD:222983"
     Region          321..368
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          372..423
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          427..471
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          474..525
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          527..577
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     CDS             1..605
                     /gene="LOC144114434"
                     /coded_by="XM_077648175.1:465..2282"
                     /db_xref="GeneID:144114434"
ORIGIN      
        1 mkevsrhsdg tvpgfrmvhf espphasall nglnllrarg qlldvtliag grefkahrvv
       61 laacsdyfra mftdamlesr qpeiclngvs aqglrclley aytsrlvlsl aniqdvlata
      121 nhvgltpvve acssylqdql dlencidvat laetyslrrl rkrvyrfica nlhqfaktpe
      181 fqrlsttqme hllacdfpvn cpegdvlsfv lswvacdvag rlsqarklvs yvcfseipas
      241 klaelwdspv lqclfsrrip phlsgiggqp ppslvntrgm elvllkvggf glsgvtneit
      301 yylpsaarwk yltsiphveq cnfgtavlgn elyvvggcfn qslhqenvhp fgfrynalig
      361 ewstmapmrw ercrfalcva rdhlyavgga gevlgdtdtp edgeahcery dphsdvwipv
      421 aplpgartqh agtawgpylf vsgglnadsv lnsllrydtr tdswetmapm stpradhsmv
      481 vysdrlvvcg gwyedgatgt rvlaesveay diatnawtpv ttvptpryha gvavlgswly
      541 tvggfhsdtt fdrasgiver fdldgnlgwe eaepypqdvw ehvcctlfvp rcrddldvis
      601 dktlm
//
LOCUS       XP_077504302             188 aa            linear   INV 25-NOV-2025
DEFINITION  plasmolipin-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504302
VERSION     XP_077504302.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648176.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..188
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..188
                     /product="plasmolipin-like isoform X1"
                     /calculated_mol_wt=20691
     Region          31..>123
                     /region_name="MARVEL"
                     /note="Membrane-associating domain; pfam01284"
                     /db_xref="CDD:366555"
     CDS             1..188
                     /gene="LOC144114436"
                     /coded_by="XM_077648176.1:215..781"
                     /db_xref="GeneID:144114436"
ORIGIN      
        1 mqgqgfpdsh tttttttttk taswlvfdps ylrtlpgilk gaqiiinlig ficsqldyyf
       61 pstsnwygfv smtafwvtlv llffylcrli ervkfvpwml aefvycgvwa tlyftaaiaa
      121 avkgryqecl aaaaffgfva milygadaff kykgwksgep aqgersvpsa glgasntvsp
      181 tspnypay
//
LOCUS       XP_077504303             163 aa            linear   INV 25-NOV-2025
DEFINITION  plasmolipin-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504303
VERSION     XP_077504303.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648177.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..163
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..163
                     /product="plasmolipin-like isoform X2"
                     /calculated_mol_wt=18258
     Region          31..>123
                     /region_name="MARVEL"
                     /note="Membrane-associating domain; pfam01284"
                     /db_xref="CDD:366555"
     CDS             1..163
                     /gene="LOC144114436"
                     /coded_by="XM_077648177.1:202..693"
                     /db_xref="GeneID:144114436"
ORIGIN      
        1 mqgqgfpdsh tttttttttk taswlvfdps ylrtlpgilk gaqiiinlig ficsqldyyf
       61 pstsnwygfv smtafwvtlv llffylcrli ervkfvpwml aefvycgvwa tlyftaaiaa
      121 avkgryqecl aaaaffgfva milygadaff kykgwksgep aqe
//
LOCUS       XP_077504305             402 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-(1,3)-fucosyltransferase C-like [Amblyomma americanum].
ACCESSION   XP_077504305
VERSION     XP_077504305.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648179.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..402
                     /product="alpha-(1,3)-fucosyltransferase C-like"
                     /calculated_mol_wt=46964
     Region          70..186
                     /region_name="Glyco_tran_10_N"
                     /note="Fucosyltransferase, N-terminal; pfam17039"
                     /db_xref="CDD:465336"
     Region          207..383
                     /region_name="Glyco_transf_10"
                     /note="Glycosyltransferase family 10 (fucosyltransferase)
                     C-term; pfam00852"
                     /db_xref="CDD:459962"
     CDS             1..402
                     /gene="LOC144114437"
                     /coded_by="XM_077648179.1:208..1416"
                     /db_xref="GeneID:144114437"
ORIGIN      
        1 msvprrhraa vvtllslsaf sllsysflpv thdylfsylr gsdyisvagg lhqnrswapf
       61 ytrtdifgrp dkprilfwtt vfgdwysgls khgtaevryw qpvggcadrc yiandrrtls
      121 vsdavvfyac dlnasdlpsq raqgqkwvlw sleaptscdl splvpmqsai nwtmtyrrds
      181 dvldgyaevt rlerpasysm qdlkwtwkrk kimavwavsh cetfgkredf vddlrrhvqv
      241 dvygkcgnls csrqvrfscy rkfareyffl lsfentvcrd yvteklymal lydiipvtfg
      301 gaeyetmapp gsyidalkfr tpkdladylk kvagdfelyk syfrwkgkyn vepwayytfc
      361 klcaklhsns frersvypni lrwwnetsyc rawnretkql yk
//
LOCUS       XP_077504306             402 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-(1,3)-fucosyltransferase C-like [Amblyomma americanum].
ACCESSION   XP_077504306
VERSION     XP_077504306.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648180.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..402
                     /product="alpha-(1,3)-fucosyltransferase C-like"
                     /calculated_mol_wt=46964
     Region          70..186
                     /region_name="Glyco_tran_10_N"
                     /note="Fucosyltransferase, N-terminal; pfam17039"
                     /db_xref="CDD:465336"
     Region          207..383
                     /region_name="Glyco_transf_10"
                     /note="Glycosyltransferase family 10 (fucosyltransferase)
                     C-term; pfam00852"
                     /db_xref="CDD:459962"
     CDS             1..402
                     /gene="LOC144114437"
                     /coded_by="XM_077648180.1:167..1375"
                     /db_xref="GeneID:144114437"
ORIGIN      
        1 msvprrhraa vvtllslsaf sllsysflpv thdylfsylr gsdyisvagg lhqnrswapf
       61 ytrtdifgrp dkprilfwtt vfgdwysgls khgtaevryw qpvggcadrc yiandrrtls
      121 vsdavvfyac dlnasdlpsq raqgqkwvlw sleaptscdl splvpmqsai nwtmtyrrds
      181 dvldgyaevt rlerpasysm qdlkwtwkrk kimavwavsh cetfgkredf vddlrrhvqv
      241 dvygkcgnls csrqvrfscy rkfareyffl lsfentvcrd yvteklymal lydiipvtfg
      301 gaeyetmapp gsyidalkfr tpkdladylk kvagdfelyk syfrwkgkyn vepwayytfc
      361 klcaklhsns frersvypni lrwwnetsyc rawnretkql yk
//
LOCUS       XP_077504307             402 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-(1,3)-fucosyltransferase C-like [Amblyomma americanum].
ACCESSION   XP_077504307
VERSION     XP_077504307.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648181.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..402
                     /product="alpha-(1,3)-fucosyltransferase C-like"
                     /calculated_mol_wt=46964
     Region          70..186
                     /region_name="Glyco_tran_10_N"
                     /note="Fucosyltransferase, N-terminal; pfam17039"
                     /db_xref="CDD:465336"
     Region          207..383
                     /region_name="Glyco_transf_10"
                     /note="Glycosyltransferase family 10 (fucosyltransferase)
                     C-term; pfam00852"
                     /db_xref="CDD:459962"
     CDS             1..402
                     /gene="LOC144114437"
                     /coded_by="XM_077648181.1:382..1590"
                     /db_xref="GeneID:144114437"
ORIGIN      
        1 msvprrhraa vvtllslsaf sllsysflpv thdylfsylr gsdyisvagg lhqnrswapf
       61 ytrtdifgrp dkprilfwtt vfgdwysgls khgtaevryw qpvggcadrc yiandrrtls
      121 vsdavvfyac dlnasdlpsq raqgqkwvlw sleaptscdl splvpmqsai nwtmtyrrds
      181 dvldgyaevt rlerpasysm qdlkwtwkrk kimavwavsh cetfgkredf vddlrrhvqv
      241 dvygkcgnls csrqvrfscy rkfareyffl lsfentvcrd yvteklymal lydiipvtfg
      301 gaeyetmapp gsyidalkfr tpkdladylk kvagdfelyk syfrwkgkyn vepwayytfc
      361 klcaklhsns frersvypni lrwwnetsyc rawnretkql yk
//
LOCUS       XP_077504308             402 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-(1,3)-fucosyltransferase C-like [Amblyomma americanum].
ACCESSION   XP_077504308
VERSION     XP_077504308.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648182.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..402
                     /product="alpha-(1,3)-fucosyltransferase C-like"
                     /calculated_mol_wt=46964
     Region          70..186
                     /region_name="Glyco_tran_10_N"
                     /note="Fucosyltransferase, N-terminal; pfam17039"
                     /db_xref="CDD:465336"
     Region          207..383
                     /region_name="Glyco_transf_10"
                     /note="Glycosyltransferase family 10 (fucosyltransferase)
                     C-term; pfam00852"
                     /db_xref="CDD:459962"
     CDS             1..402
                     /gene="LOC144114437"
                     /coded_by="XM_077648182.1:125..1333"
                     /db_xref="GeneID:144114437"
ORIGIN      
        1 msvprrhraa vvtllslsaf sllsysflpv thdylfsylr gsdyisvagg lhqnrswapf
       61 ytrtdifgrp dkprilfwtt vfgdwysgls khgtaevryw qpvggcadrc yiandrrtls
      121 vsdavvfyac dlnasdlpsq raqgqkwvlw sleaptscdl splvpmqsai nwtmtyrrds
      181 dvldgyaevt rlerpasysm qdlkwtwkrk kimavwavsh cetfgkredf vddlrrhvqv
      241 dvygkcgnls csrqvrfscy rkfareyffl lsfentvcrd yvteklymal lydiipvtfg
      301 gaeyetmapp gsyidalkfr tpkdladylk kvagdfelyk syfrwkgkyn vepwayytfc
      361 klcaklhsns frersvypni lrwwnetsyc rawnretkql yk
//
LOCUS       XP_077504309             402 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-(1,3)-fucosyltransferase C-like [Amblyomma americanum].
ACCESSION   XP_077504309
VERSION     XP_077504309.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648183.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..402
                     /product="alpha-(1,3)-fucosyltransferase C-like"
                     /calculated_mol_wt=46964
     Region          70..186
                     /region_name="Glyco_tran_10_N"
                     /note="Fucosyltransferase, N-terminal; pfam17039"
                     /db_xref="CDD:465336"
     Region          207..383
                     /region_name="Glyco_transf_10"
                     /note="Glycosyltransferase family 10 (fucosyltransferase)
                     C-term; pfam00852"
                     /db_xref="CDD:459962"
     CDS             1..402
                     /gene="LOC144114437"
                     /coded_by="XM_077648183.1:301..1509"
                     /db_xref="GeneID:144114437"
ORIGIN      
        1 msvprrhraa vvtllslsaf sllsysflpv thdylfsylr gsdyisvagg lhqnrswapf
       61 ytrtdifgrp dkprilfwtt vfgdwysgls khgtaevryw qpvggcadrc yiandrrtls
      121 vsdavvfyac dlnasdlpsq raqgqkwvlw sleaptscdl splvpmqsai nwtmtyrrds
      181 dvldgyaevt rlerpasysm qdlkwtwkrk kimavwavsh cetfgkredf vddlrrhvqv
      241 dvygkcgnls csrqvrfscy rkfareyffl lsfentvcrd yvteklymal lydiipvtfg
      301 gaeyetmapp gsyidalkfr tpkdladylk kvagdfelyk syfrwkgkyn vepwayytfc
      361 klcaklhsns frersvypni lrwwnetsyc rawnretkql yk
//
LOCUS       XP_077504310             155 aa            linear   INV 25-NOV-2025
DEFINITION  selenoprotein F [Amblyomma americanum].
ACCESSION   XP_077504310
VERSION     XP_077504310.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648184.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..155
                     /product="selenoprotein F"
                     /calculated_mol_wt=17349
     Region          79..153
                     /region_name="Sep15_SelM"
                     /note="Sep15/SelM redox domain; pfam08806"
                     /db_xref="CDD:462607"
     CDS             1..155
                     /gene="LOC144114439"
                     /coded_by="XM_077648184.1:70..537"
                     /db_xref="GeneID:144114439"
ORIGIN      
        1 matvyvllqi lvapltvlal dltgglsaed clargfrkpd licsscrilp nfeldslked
       61 cnkccvqtee rgtakrypra vlevcackfg hmpqieafcr gpkpkqfpnl tikylrgadp
      121 iiklydenge vqeelsikkw dtdtieeflf ehlap
//
LOCUS       XP_077504311            2820 aa            linear   INV 25-NOV-2025
DEFINITION  multiple EGF like domains 8 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504311
VERSION     XP_077504311.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648185.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2820
                     /product="multiple EGF like domains 8 isoform X1"
                     /calculated_mol_wt=306075
     Region          43..143
                     /region_name="CUB"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; cl00049"
                     /db_xref="CDD:412131"
     Site            order(47,49,51,77,82,116,140,142,144..145)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     Region          246..560
                     /region_name="NanM"
                     /note="N-acetylneuraminic acid mutarotase [Cell
                     wall/membrane/envelope biogenesis]; COG3055"
                     /db_xref="CDD:442289"
     Region          248..296
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          300..345
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          348..397
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          407..462
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          1133..1167
                     /region_name="EGF_3"
                     /note="EGF domain; pfam12947"
                     /db_xref="CDD:463759"
     Region          1205..1250
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1205,1207,1213,1220,1223,1232)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1252..1301
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1252,1254,1265,1272,1274,1283)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1545..1594
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          1554..1604
                     /region_name="Kelch_3"
                     /note="Galactose oxidase, central domain; pfam13415"
                     /db_xref="CDD:433188"
     Region          1589..>1836
                     /region_name="NanM"
                     /note="N-acetylneuraminic acid mutarotase [Cell
                     wall/membrane/envelope biogenesis]; COG3055"
                     /db_xref="CDD:442289"
     Region          1598..1642
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          1645..1694
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          1761..1814
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          2278..2325
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(2278,2280,2290,2295,2298,2307)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2407..2472
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(2408,2410,2417,2425,2427,2454)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     CDS             1..2820
                     /gene="Megf8"
                     /coded_by="XM_077648185.1:530..8992"
                     /db_xref="GeneID:144114440"
ORIGIN      
        1 matwlllpsg agallvplll awcvsgpaaa kpsdtcnrhr kiltapwgii tdgndsyrnd
       61 shcewliran ssklyitlrf hematecayd hvfvydgdsy sspllgtfsg esipppviat
      121 sgsmlillys dtnyvlkgfv aeysitqcps ncsnhgscgr dscvcdlawt gpdcstpvcp
      181 sschadqgqg ncsfpcttts ppgsseipnc crcshgfagi scslpenpnd saagwhllsr
      241 gggpfaarag hagvylpftd qlfvfggraf grvlgdflvy nfnesawhdl tdvrprpaar
      301 wghamapyah tialhggelt ngslsselwl ynvakrewtl elpspaghyp pavalhsltp
      361 gdgnhlyvfg grtaggvfsd alyrvnprng tqwervayvg svppsvghaa vfhaesrsll
      421 vhggvvvdya rfsklssalw afhvdanrwt rlqrtplgge apterafhaa alvggymvlf
      481 ggymhkhsfe ekcfddgiyl yhlrchkwvs fkliagdllg hvhgercpgv yshvmalrrg
      541 htllvlggyr gvmssdlwaf llpttithay sdsasgacir hrfetacqan pacawcggsg
      601 vcldrrkvkd crsgnfrpgs cpglcaalgd cqscmiwggh anmpvgsndl rglsaktsfp
      661 qacgwcveaa tchpmneppg vcrppqngqi speqagfwgp hghivtsvye crildfrpgl
      721 fllqyrnpan lsqpdqvsyv nstsqqlvft seyqddqegg gehmarllgf lhprgalpps
      781 geplrlfldi sggraalrlg qagntrledt elvaqlsahs ynrteakrpn gqtllpstam
      841 relrylldls lhtpakyctk hcdrsldlrw nalasqqvis skhlepyrng tqcpsrgtcl
      901 aclsdagcgw cpsasfcvpr qpapdpvakg talpcapgss qllilephhc ptcsafiych
      961 dcvrdpfcew ivegafcvrr grhssavkhp dlcptpchlr rgcisclgdp grcawcretq
     1021 scflfstytt sfmyggcrew vdedhgspkg atfpgaqcpn csafkdcagc lqklgcgwcg
     1081 ndynpnngvc vegdfsgpyl gtcservrkq fpslggprea swsyaqcpnv necklklarc
     1141 hpdascydta nsyrcqcnrg fkgdgtttcv ktcaehcvhg hcseapdfrc ichlgwtgpr
     1201 cdvdcgchnh sscergvghc drcynltega hchlcrrgsf gnatspfgcr kcacnghgda
     1261 tqgmcdsstg dcyclhntqg khcetcqpgy fgdprnggrc yidcspkriv yvesgggglg
     1321 vpdmegpmea hhclwiltst sassnntlsl hrgatleitl lpnlripcpe nhvyvysgvp
     1381 pfvlsavrgs eesvllgafc gtnltapitl vsptgwlsvy frrgrlpqgf satfrrldcp
     1441 iaclsepqrv csasgecacs rgyvgsrcqv aicpnecsas qhhglcekgy rlchcgdqyg
     1501 aadcgqalvp gqvswsllfe lerltgqvqg gglggtselp hsclghslls lepnllwlfg
     1561 gmdpargdsd elyvydldfd rwkklnvsre vgqaptarrf hgaamvagsm yifgglnatg
     1621 vlgdfwkfvt dlytwtript tpdvpalagl tltnvdnqql vliggfspey gfqdktlays
     1681 ieamdwrvln ttgtvpigvy ghtsnyhqpt qsiyvfggiv ysvdrplvsd hlyvlhmpsr
     1741 rwsrlppddr anpaysrisp ryfhasaste nslfilggrn gsgeailepf aydfscnhwi
     1801 pldgpyvqfv gdqpaalvga satllqgkly vlggrsvrlq rvtlprdlcg lfshsrlgcl
     1861 rsvgcsycsv qdsqgnithc yssartvpps cihhrggtle vaqgkvcdad wlgnrtcyqy
     1921 nncedcvasw pahpgvqhtc ewctgcrkgr cfragessce kpigcdgprh plvsdpaqcp
     1981 lrsclasece kcrdlgscmw trqvvrssel ghtvnvrpif dwtcvgrtlk dassfpvesn
     2041 pprpcpkrcs qhtsctecla saggeggwhe crwssalaec mtpsyvglrc eggacgqvlg
     2101 gsvsscgvpc hvqgqaahcl rdprcgwcgl rapgangrgl cmrgghrgpt ggscgpasvf
     2161 lhghplptnv shwlqtlgse vdwyyfvrpp enectnghht cdsereecvd tadgfecvck
     2221 qgyaisssss kssgagssag spmcepvcpe gcdqgqcvrp neckcnfgyv grncstlchc
     2281 nghsdcaspd hldvctkchn ntkgpqcshc epfyvgnpak ggrcvpcley cnqhtnicve
     2341 agqldfnesa gnlspaefle sfkqiehgpl elavcrfcqn ntmgaqceec vegffvvgkd
     2401 iargcrpcec hghgdtcdrr tgencncknn tendrqcsqk sgknsaspcw nlqcskcken
     2461 fqgvptnghq cyrhmrleqd ycldpetqec spdpnllpqg rtmffvvqpr ymnvdiriim
     2521 ditsgaadfy lsakedtfvv evnrtsgahv vsvdrkygvg ipgdlaasaa vpaaaslrns
     2581 sdatwllrpr hatpvglaty vtvrdprefl lvqnlqhrlv itvpqnvydl rstrlylvia
     2641 gaseiptrgs lffrqdqtri dlfvffsvff scfflflaac vvvwkvkraf dirrarrlha
     2701 aqmehmarrp fakvclsild deengdnfrs plrkktqrra lrdspkgvpa pcgsvrpvav
     2761 eptgdgaaav ttllvqlpgg lsapvrlala salvavrsgg ggaaggiraa mrrrtshinl
//
LOCUS       XP_077504312            2819 aa            linear   INV 25-NOV-2025
DEFINITION  multiple EGF like domains 8 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504312
VERSION     XP_077504312.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648186.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2819
                     /product="multiple EGF like domains 8 isoform X2"
                     /calculated_mol_wt=305988
     Region          43..143
                     /region_name="CUB"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; cl00049"
                     /db_xref="CDD:412131"
     Site            order(47,49,51,77,82,116,140,142,144..145)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     Region          246..560
                     /region_name="NanM"
                     /note="N-acetylneuraminic acid mutarotase [Cell
                     wall/membrane/envelope biogenesis]; COG3055"
                     /db_xref="CDD:442289"
     Region          248..296
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          300..345
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          348..397
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          407..462
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          1133..1167
                     /region_name="EGF_3"
                     /note="EGF domain; pfam12947"
                     /db_xref="CDD:463759"
     Region          1205..1250
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1205,1207,1213,1220,1223,1232)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1252..1301
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1252,1254,1265,1272,1274,1283)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1545..1594
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          1554..1604
                     /region_name="Kelch_3"
                     /note="Galactose oxidase, central domain; pfam13415"
                     /db_xref="CDD:433188"
     Region          1589..>1836
                     /region_name="NanM"
                     /note="N-acetylneuraminic acid mutarotase [Cell
                     wall/membrane/envelope biogenesis]; COG3055"
                     /db_xref="CDD:442289"
     Region          1598..1642
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          1645..1694
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          1761..1814
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          2277..2324
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(2277,2279,2289,2294,2297,2306)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2406..2471
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(2407,2409,2416,2424,2426,2453)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     CDS             1..2819
                     /gene="Megf8"
                     /coded_by="XM_077648186.1:530..8989"
                     /db_xref="GeneID:144114440"
ORIGIN      
        1 matwlllpsg agallvplll awcvsgpaaa kpsdtcnrhr kiltapwgii tdgndsyrnd
       61 shcewliran ssklyitlrf hematecayd hvfvydgdsy sspllgtfsg esipppviat
      121 sgsmlillys dtnyvlkgfv aeysitqcps ncsnhgscgr dscvcdlawt gpdcstpvcp
      181 sschadqgqg ncsfpcttts ppgsseipnc crcshgfagi scslpenpnd saagwhllsr
      241 gggpfaarag hagvylpftd qlfvfggraf grvlgdflvy nfnesawhdl tdvrprpaar
      301 wghamapyah tialhggelt ngslsselwl ynvakrewtl elpspaghyp pavalhsltp
      361 gdgnhlyvfg grtaggvfsd alyrvnprng tqwervayvg svppsvghaa vfhaesrsll
      421 vhggvvvdya rfsklssalw afhvdanrwt rlqrtplgge apterafhaa alvggymvlf
      481 ggymhkhsfe ekcfddgiyl yhlrchkwvs fkliagdllg hvhgercpgv yshvmalrrg
      541 htllvlggyr gvmssdlwaf llpttithay sdsasgacir hrfetacqan pacawcggsg
      601 vcldrrkvkd crsgnfrpgs cpglcaalgd cqscmiwggh anmpvgsndl rglsaktsfp
      661 qacgwcveaa tchpmneppg vcrppqngqi speqagfwgp hghivtsvye crildfrpgl
      721 fllqyrnpan lsqpdqvsyv nstsqqlvft seyqddqegg gehmarllgf lhprgalpps
      781 geplrlfldi sggraalrlg qagntrledt elvaqlsahs ynrteakrpn gqtllpstam
      841 relrylldls lhtpakyctk hcdrsldlrw nalasqqvis skhlepyrng tqcpsrgtcl
      901 aclsdagcgw cpsasfcvpr qpapdpvakg talpcapgss qllilephhc ptcsafiych
      961 dcvrdpfcew ivegafcvrr grhssavkhp dlcptpchlr rgcisclgdp grcawcretq
     1021 scflfstytt sfmyggcrew vdedhgspkg atfpgaqcpn csafkdcagc lqklgcgwcg
     1081 ndynpnngvc vegdfsgpyl gtcservrkq fpslggprea swsyaqcpnv necklklarc
     1141 hpdascydta nsyrcqcnrg fkgdgtttcv ktcaehcvhg hcseapdfrc ichlgwtgpr
     1201 cdvdcgchnh sscergvghc drcynltega hchlcrrgsf gnatspfgcr kcacnghgda
     1261 tqgmcdsstg dcyclhntqg khcetcqpgy fgdprnggrc yidcspkriv yvesgggglg
     1321 vpdmegpmea hhclwiltst sassnntlsl hrgatleitl lpnlripcpe nhvyvysgvp
     1381 pfvlsavrgs eesvllgafc gtnltapitl vsptgwlsvy frrgrlpqgf satfrrldcp
     1441 iaclsepqrv csasgecacs rgyvgsrcqv aicpnecsas qhhglcekgy rlchcgdqyg
     1501 aadcgqalvp gqvswsllfe lerltgqvqg gglggtselp hsclghslls lepnllwlfg
     1561 gmdpargdsd elyvydldfd rwkklnvsre vgqaptarrf hgaamvagsm yifgglnatg
     1621 vlgdfwkfvt dlytwtript tpdvpalagl tltnvdnqql vliggfspey gfqdktlays
     1681 ieamdwrvln ttgtvpigvy ghtsnyhqpt qsiyvfggiv ysvdrplvsd hlyvlhmpsr
     1741 rwsrlppddr anpaysrisp ryfhasaste nslfilggrn gsgeailepf aydfscnhwi
     1801 pldgpyvqfv gdqpaalvga satllqgkly vlggrsvrlq rvtlprdlcg lfshsrlgcl
     1861 rsvgcsycsv qdsqgnithc yssartvpps cihhrggtle vaqgkvcdad wlgnrtcyqy
     1921 nncedcvasw pahpgvqhtc ewctgcrkgr cfragessce kpigcdgprh plvsdpaqcp
     1981 lrsclasece kcrdlgscmw trqvvrssel ghtvnvrpif dwtcvgrtlk dassfpvesn
     2041 pprpcpkrcs qhtsctecla saggeggwhe crwssalaec mtpsyvglrc eggacgqvlg
     2101 gsvsscgvpc hvqgqaahcl rdprcgwcgl rapgangrgl cmrgghrgpt ggscgpasvf
     2161 lhghplptnv shwlqtlgse vdwyyfvrpp enectnghht cdsereecvd tadgfecvck
     2221 qgyaissssk ssgagssags pmcepvcpeg cdqgqcvrpn eckcnfgyvg rncstlchcn
     2281 ghsdcaspdh ldvctkchnn tkgpqcshce pfyvgnpakg grcvpcleyc nqhtnicvea
     2341 gqldfnesag nlspaefles fkqiehgple lavcrfcqnn tmgaqceecv egffvvgkdi
     2401 argcrpcech ghgdtcdrrt gencncknnt endrqcsqks gknsaspcwn lqcskckenf
     2461 qgvptnghqc yrhmrleqdy cldpetqecs pdpnllpqgr tmffvvqpry mnvdiriimd
     2521 itsgaadfyl sakedtfvve vnrtsgahvv svdrkygvgi pgdlaasaav paaaslrnss
     2581 datwllrprh atpvglatyv tvrdprefll vqnlqhrlvi tvpqnvydlr strlylviag
     2641 aseiptrgsl ffrqdqtrid lfvffsvffs cfflflaacv vvwkvkrafd irrarrlhaa
     2701 qmehmarrpf akvclsildd eengdnfrsp lrkktqrral rdspkgvpap cgsvrpvave
     2761 ptgdgaaavt tllvqlpggl sapvrlalas alvavrsggg gaaggiraam rrrtshinl
//
LOCUS       XP_077504313             294 aa            linear   INV 25-NOV-2025
DEFINITION  phenazine biosynthesis-like domain-containing protein [Amblyomma
            americanum].
ACCESSION   XP_077504313
VERSION     XP_077504313.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648187.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..294
                     /product="phenazine biosynthesis-like domain-containing
                     protein"
                     /calculated_mol_wt=31736
     Region          9..294
                     /region_name="YHI9"
                     /note="Predicted epimerase YddE/YHI9, PhzF superfamily
                     [General function prediction only]; COG0384"
                     /db_xref="CDD:440153"
     CDS             1..294
                     /gene="LOC144114442"
                     /coded_by="XM_077648187.1:81..965"
                     /db_xref="GeneID:144114442"
ORIGIN      
        1 mqvasgsgrl plfvvdaftk apfsgnpaav vflqddseid nhrkqsiaae mkhsetafvs
       61 klnaqdqfdk ascfnlrwft pqnevalcgh atlataaaif gewgnpsqel qfhtksgvlk
      121 askepdgail ldlpartsvt veeakyrsll davlgdvpta elrysaeekk lvvrlhdrct
      181 rsqlealkps paemlraend srvrgvavtv rgggdydfls ryfspwngip edpvtgsaht
      241 vlgpywaavl gkeelkahqa sarggdlflr vptgghrlqi ggdaavvlrg elli
//
LOCUS       XP_077504314             218 aa            linear   INV 25-NOV-2025
DEFINITION  vesicle-associated membrane protein 7-like [Amblyomma americanum].
ACCESSION   XP_077504314
VERSION     XP_077504314.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648188.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..218
                     /product="vesicle-associated membrane protein 7-like"
                     /calculated_mol_wt=24371
     Region          7..119
                     /region_name="Longin"
                     /note="longin domain; cd14824"
                     /db_xref="CDD:341428"
     Site            order(16,19,21,28,32,112,116,119)
                     /site_type="other"
                     /note="Sec22 interface [polypeptide binding]"
                     /db_xref="CDD:341428"
     Site            order(27,30,50,53,55)
                     /site_type="lipid-binding"
                     /note="lipid binding site [chemical binding]"
                     /db_xref="CDD:341428"
     Site            order(49,52,72,76)
                     /site_type="other"
                     /note="VARP interface [polypeptide binding]"
                     /db_xref="CDD:341428"
     Region          128..214
                     /region_name="Synaptobrevin"
                     /note="pfam00957"
                     /db_xref="CDD:395764"
     CDS             1..218
                     /gene="LOC144114444"
                     /coded_by="XM_077648188.1:164..820"
                     /db_xref="GeneID:144114444"
ORIGIN      
        1 mvqsieycai ardatvlvsh qetsadfeyl lsdvlreipp hadfkttrsr ggyvfhllve
       61 qglvyicatl peasmhqsft clflikqrfl egslssrawt aqeheldrdf gcviadimrn
      121 cnsgrtgdqi sqlhrqvedv rgimaqnier vvergdrlds llektqdleq agtvfkatak
      181 kvnrhmclrn armmivigii vagvitlitl fatgvikt
//
LOCUS       XP_077504316             218 aa            linear   INV 25-NOV-2025
DEFINITION  vesicle-associated membrane protein 7-like [Amblyomma americanum].
ACCESSION   XP_077504316
VERSION     XP_077504316.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648190.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..218
                     /product="vesicle-associated membrane protein 7-like"
                     /calculated_mol_wt=24371
     Region          7..119
                     /region_name="Longin"
                     /note="longin domain; cd14824"
                     /db_xref="CDD:341428"
     Site            order(16,19,21,28,32,112,116,119)
                     /site_type="other"
                     /note="Sec22 interface [polypeptide binding]"
                     /db_xref="CDD:341428"
     Site            order(27,30,50,53,55)
                     /site_type="lipid-binding"
                     /note="lipid binding site [chemical binding]"
                     /db_xref="CDD:341428"
     Site            order(49,52,72,76)
                     /site_type="other"
                     /note="VARP interface [polypeptide binding]"
                     /db_xref="CDD:341428"
     Region          128..214
                     /region_name="Synaptobrevin"
                     /note="pfam00957"
                     /db_xref="CDD:395764"
     CDS             1..218
                     /gene="LOC144114444"
                     /coded_by="XM_077648190.1:261..917"
                     /db_xref="GeneID:144114444"
ORIGIN      
        1 mvqsieycai ardatvlvsh qetsadfeyl lsdvlreipp hadfkttrsr ggyvfhllve
       61 qglvyicatl peasmhqsft clflikqrfl egslssrawt aqeheldrdf gcviadimrn
      121 cnsgrtgdqi sqlhrqvedv rgimaqnier vvergdrlds llektqdleq agtvfkatak
      181 kvnrhmclrn armmivigii vagvitlitl fatgvikt
//
LOCUS       XP_077504317             427 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114445 [Amblyomma americanum].
ACCESSION   XP_077504317
VERSION     XP_077504317.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648191.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..427
                     /product="uncharacterized protein LOC144114445"
                     /calculated_mol_wt=44402
     Region          <355..>393
                     /region_name="KCT2"
                     /note="Keratinocyte-associated gene product; pfam17818"
                     /db_xref="CDD:407686"
     CDS             1..427
                     /gene="LOC144114445"
                     /coded_by="XM_077648191.1:285..1568"
                     /db_xref="GeneID:144114445"
ORIGIN      
        1 mgvfklssrt acfllwlfiv hsvqvcaltv psiaskkegs eevarkaaaa nagiekdinr
       61 pdasnaaapl gvehseskkp vdsggsgdpi kvvqseptst aeskakwesk dvqsplnrha
      121 qvksnqghrd rvesgesaqk psmspakpla pkpasapvve qqeskvghpa stgsdkstan
      181 aavdvgkmkn sgvnntakhp edektsvkkp legsaatvga slaatsette tttlaqektl
      241 aattlkpaep ahlvasehak llpgapsqgq gvekkysgtv pdvpkdfggm asastgnvey
      301 gqldsgedlg pplptqknpa vstsvqlgaq paagsvggsr pekpldasaa agsmpllpaq
      361 ddshffayfl tavvlcvvgy lafhnkrkil alilegrher qrrhnghyrr ldsvddasav
      421 rkgrgsf
//
LOCUS       XP_077504318             279 aa            linear   INV 25-NOV-2025
DEFINITION  inositol polyphosphate multikinase-like [Amblyomma americanum].
ACCESSION   XP_077504318
VERSION     XP_077504318.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648192.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..279
                     /product="inositol polyphosphate multikinase-like"
                     /calculated_mol_wt=32124
     Region          96..274
                     /region_name="IPK"
                     /note="Inositol polyphosphate kinase; pfam03770"
                     /db_xref="CDD:397715"
     CDS             1..279
                     /gene="LOC144114446"
                     /coded_by="XM_077648192.1:325..1164"
                     /db_xref="GeneID:144114446"
ORIGIN      
        1 mkegtplpdg tllfdyqiag hvhgskktkl gllkhkdgsi lkpllscder tlrehkfyer
       61 vfsaqesppe llnlraflpk ylgtwktnfl gqeveylrle dvtrefrrps vmdvkigaqt
      121 ydplatpeka aledakypws rqlgfrilgm rvfdkheqky ciygkdyglk qtpdtildaf
      181 qtflgmtlqq pnvptflpdl laqlehirrw fetqhqyafy sssvlivyes qtdssdskav
      241 rcvakmidfa hvfptleydk nylhglckli svlreiaha
//
LOCUS       XP_077504319             152 aa            linear   INV 25-NOV-2025
DEFINITION  nuclear cap-binding protein subunit 2 [Amblyomma americanum].
ACCESSION   XP_077504319
VERSION     XP_077504319.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648193.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..152
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..152
                     /product="nuclear cap-binding protein subunit 2"
                     /calculated_mol_wt=17304
     Region          30..107
                     /region_name="RRM_NCBP2"
                     /note="RNA recognition motif (RRM) found in nuclear
                     cap-binding protein subunit 2 (CBP20) and similar
                     proteins; cd12240"
                     /db_xref="CDD:409686"
     Site            order(31,71,73,100,102..104)
                     /site_type="other"
                     /note="chemical substrate binding site [chemical binding]"
                     /db_xref="CDD:409686"
     Site            order(38,41..43,45..47,50..51,53,55,57,77,84,87..88,
                     90..93,95..96)
                     /site_type="other"
                     /note="subunit interface [polypeptide binding]"
                     /db_xref="CDD:409686"
     CDS             1..152
                     /gene="LOC144114447"
                     /coded_by="XM_077648193.1:132..590"
                     /db_xref="GeneID:144114447"
ORIGIN      
        1 mtadlssyrd qhfkgsraeq erllrvsstl yignmsfytt eeqiyelfsk cgdvkkvimg
       61 ldrfhktpcg fcfveyytra daenamryin gtklddriir tdwdagfmeg rqygrgksgg
      121 qvrdeyrtdy dsgrggygkl maqrvvppaa pv
//
LOCUS       XP_077504320             245 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: ribosomal RNA processing protein 36 homolog
            [Amblyomma americanum].
ACCESSION   XP_077504320
VERSION     XP_077504320.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648194.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            internal stop codons :: corrected 1 genomic stop codon
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..245
                     /product="LOW QUALITY PROTEIN: ribosomal RNA processing
                     protein 36 homolog"
                     /calculated_mol_wt=28693
     Region          76..231
                     /region_name="RRP36"
                     /note="rRNA biogenesis protein RRP36; pfam06102"
                     /db_xref="CDD:461829"
     CDS             1..245
                     /gene="LOC144114448"
                     /coded_by="XM_077648194.1:236..973"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: substituted 1 base at 1
                     genomic stop codon"
                     /db_xref="GeneID:144114448"
ORIGIN      
        1 masmalkkdt ydssdsssss edehergeks eedmssslph mpfdahqalk ekiglkmyee
       61 avfgsdrrks kqhftrenkn rpremsakrq vpvfrevfqv kkkvhqdprf sastgefdek
      121 vfresysfin dirakerdev aamvrhekdp qrrmtlqkll kkmenqeate kmkrvkanlv
      181 xavrqflash rerkpiylnk ttkrkielae rfkqlkkagk lekylekkrk knvqkerkrl
      241 pssgt
//
LOCUS       XP_077504321             456 aa            linear   INV 25-NOV-2025
DEFINITION  protein odr-4 homolog [Amblyomma americanum].
ACCESSION   XP_077504321
VERSION     XP_077504321.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648195.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..456
                     /product="protein odr-4 homolog"
                     /calculated_mol_wt=49533
     Region          29..398
                     /region_name="ODR4-like"
                     /note="Odorant response abnormal 4-like; pfam14778"
                     /db_xref="CDD:464311"
     CDS             1..456
                     /gene="LOC144114450"
                     /coded_by="XM_077648195.1:200..1570"
                     /db_xref="GeneID:144114450"
ORIGIN      
        1 mgrtvayddh vqklinelvq tkgssltvgv iigqctqqrd ivvhiantpk kdddesqeeg
       61 dvkqshksah ghadieeawi cqhakqvtrm lpggmdvlgl fvvgtpeela avqtkfrqvl
      121 favfkviakd qrtslnseit driqlqicav trkitcktfd vldhkcaarp adwkpavgac
      181 awhrleasls ldlwvalsee rcrqgllrqi qvglepfmrg lhdarallng earppdepla
      241 aatdrrrspr atkdapqslf lvdlllpldc gsgggspsiq vqgptscgas msivgtiqcr
      301 gfvhakatvq eavkaikqdv irsitsrcei qcedmllied eqhdpslvhe lpkrvfaplg
      361 drgltlcdyi fhgdaacdsl ealqellgld vtagdieldt ehspesnqqv idgvetaeii
      421 teateqlsrg rnmlipiasa aiaalgagis ylllqd
//
LOCUS       XP_077504322             449 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin alpha chain-like [Amblyomma americanum].
ACCESSION   XP_077504322
VERSION     XP_077504322.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648196.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..449
                     /product="tubulin alpha chain-like"
                     /calculated_mol_wt=49496
     Region          1..448
                     /region_name="PTZ00335"
                     /note="tubulin alpha chain; Provisional"
                     /db_xref="CDD:185562"
     CDS             1..449
                     /gene="LOC144114452"
                     /coded_by="XM_077648196.1:96..1445"
                     /db_xref="GeneID:144114452"
ORIGIN      
        1 mrecisvhig qagvqignac welyclehgi qpdgkipsdk vsggvddsfn tffaqtgagk
       61 hvpravfvdl eptvvdevrt gshkqlyhpe qlisgkedaa nnyarghyti gkeivdlvld
      121 rirkladqcs glqgflvfhs fgggtgsgft sllmqrlsvd ygkksklefs vypapqvata
      181 vvepynsilt thttlehsdc sfmvdneaiy dicrrnldid rptysnlnrl isqivssita
      241 slrfdgalnv dltefqtnlv pyprihfplv tyspvisaek ahheqltvae itsscfepsn
      301 qmvkcdprrg kymaccmlyr gdvvpkdvna aiaaikskrs iqfvdwcptg fkvginyqpp
      361 tvvpggdlak vpravcmlsn ttaiaeawgr lnhkfdlmya krafvhwyvg egmeegefae
      421 aredlaalek dyeevgadsv daaedgeef
//
LOCUS       XP_077504323             996 aa            linear   INV 25-NOV-2025
DEFINITION  serine/threonine-protein kinase 31-like [Amblyomma americanum].
ACCESSION   XP_077504323
VERSION     XP_077504323.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648197.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..996
                     /product="serine/threonine-protein kinase 31-like"
                     /calculated_mol_wt=110286
     Region          65..136
                     /region_name="Tudor_SF"
                     /note="Tudor domain superfamily; cl02573"
                     /db_xref="CDD:470623"
     Site            order(89,94,96,113,116,118)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:410449"
     Region          <313..>573
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <788..973
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     CDS             1..996
                     /gene="LOC144114453"
                     /coded_by="XM_077648197.1:85..3075"
                     /db_xref="GeneID:144114453"
ORIGIN      
        1 mqrkmskaea lasilaahgi rgprgllsna dgtevelivs vvedvvsffg lqpsdtkevv
       61 glaarlaefc ptappvknpe cnkvyaavys kdgkwyrckv lkkldctksy vqyldygnce
      121 ernheqdlvi ipnelseirp laifctfdgl vglreddnpa afrkamrelq klltdktvvv
      181 ktkgpfsvqd tnfvtscrll dsgkdivqel inqgycqktl aankdstevn ikesaapsms
      241 llskdfqwrp nqgsdsvrdg sqgyshgsep glhsglcasl kqqpaqlpgp gvsptagfip
      301 klpmdphgeh ialskqleag dnsaasveql rselnqlqve leakksqikv lqrqvaskgn
      361 vgesftsqlq glitlvekvs lqrerfstdp cvkdvieeal dvhssfgwit epihsvetai
      421 ahhqaaqeai gncvekdqle vlvakrdaaf ngllqelqpf kevseeaeqt vdvqrvesal
      481 gklkrvygvl lknsgdlssd tnsvtlslnd cyqilkqkeg pdicklrqqt esaqrnlaag
      541 lqelqqvllg vtadssgkdf flndldtsma hfhisvedem saynllfsds hgliatlvrr
      601 lvaalqdiid qaassssvqs ryrsllweif eqkeswhniq qkareasqlh kdlrklksal
      661 rhrladfeda eedqevaakv aadinairgr ilasyieqel lmvelcqahn rrfpelavly
      721 peldfplhqk yncllkpdwd lyafsvkksg cvlkttfand dmiiaeyald rpedldkllv
      781 kasdynlvss phlirikavf qskdqchayl lmpdigtpma elgrpvphlv lqqvlsalsa
      841 lhaktpnkpa iphghihpgw llvpasdrpv clglpnfqlg ksyqlplvdg idftapelry
      901 snaeptpasd mfafgclmlw vlypeavrrq sfpssldsvr emqqrekedl viiqslvnke
      961 prlrptaael llrpffaslv sqhdtasgap sfgmpt
//
LOCUS       XP_077504325             406 aa            linear   INV 25-NOV-2025
DEFINITION  stomatin-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077504325
VERSION     XP_077504325.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648199.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..406
                     /product="stomatin-like protein 1"
                     /calculated_mol_wt=45121
     Region          109..239
                     /region_name="SPFH_like"
                     /note="core domain of the SPFH (stomatin, prohibitin,
                     flotillin, and HflK/C) superfamily; cl19107"
                     /db_xref="CDD:473137"
     Region          305..402
                     /region_name="SCP2"
                     /note="Putative sterol carrier protein, contains SCP2
                     domain [Lipid transport and metabolism]; COG3255"
                     /db_xref="CDD:442486"
     CDS             1..406
                     /gene="LOC144114456"
                     /coded_by="XM_077648199.1:93..1313"
                     /db_xref="GeneID:144114456"
ORIGIN      
        1 mdgkfspyrd nydtpdfvfd ytsafdyssi hhmtalkkpe kyktifnynk rqvvvdlsdc
       61 epepwqstii rtivtffcyl flvftfpvtc wvcikrvpsl eriavfrlgk lqpvqgpglv
      121 lvfpvidsym kvdlkpkvfe vptrevltgd gaiievgael hwqvvhsvry vtrvkevdat
      181 vgalcqqcla sllgcsdqdd idrrreaiea tlltklneti lpwglevtkv tlkvarvvkt
      241 aepsnpltpv msalksalgf psehtkiqts vpsgpgglha vpaspsepqf eqlvqvlhql
      301 alfaaqqeef qgvyatyglq ihggtkdgit vhicfdqgnv elirgsdlgs rrkadvtlrl
      361 saddltlilc gkespmqvym ggriqvegdw sclrrfmgvi dkcsng
//
LOCUS       XP_077504326            1161 aa            linear   INV 25-NOV-2025
DEFINITION  HECT and RLD domain containing E3 ubiquitin ligase 4 isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077504326
VERSION     XP_077504326.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648200.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1161
                     /product="HECT and RLD domain containing E3 ubiquitin
                     ligase 4 isoform X1"
                     /calculated_mol_wt=128109
     Region          31..375
                     /region_name="ATS1"
                     /note="Alpha-tubulin suppressor ATS1 and related RCC1
                     domain-containing proteins [Cell cycle control, cell
                     division, chromosome partitioning, Cytoskeleton]; COG5184"
                     /db_xref="CDD:444065"
     Region          813..1159
                     /region_name="HECTc"
                     /note="HECT domain; C-terminal catalytic domain of a
                     subclass of Ubiquitin-protein ligase (E3). It binds
                     specific ubiquitin-conjugating enzymes (E2), accepts
                     ubiquitin from E2, transfers ubiquitin to substrate lysine
                     side chains, and transfers additional...; cd00078"
                     /db_xref="CDD:238033"
     Site            order(819,849,860,917,1094,1123..1124,1127..1131,1151,
                     1158)
                     /site_type="active"
                     /note="catalytic cleft [active]"
                     /db_xref="CDD:238033"
     Site            order(945,948..949,951..952,955,962,964,968..969,971,978,
                     983,1000,1004)
                     /site_type="active"
                     /note="E2 interaction site [active]"
                     /db_xref="CDD:238033"
     CDS             1..1161
                     /gene="Herc4"
                     /coded_by="XM_077648200.1:235..3720"
                     /db_xref="GeneID:144114457"
ORIGIN      
        1 mrrshsdtaa ggsgrtspsl gpigpgapmm lyswglgphg elgltqgkdg vdtetavcqp
       61 ervrrfgtra vrevacgqkh tlvllangsv yscgsndfgq lgqdrsmrkl eqvaalaahe
      121 iravacgevh slalsdagqv fswgcnrngq lgcgtedesr lrpkvikkla tlhvvqiacg
      181 svhclalann gelyswggna ygqlglgrds sgderlprrv thlggvplaq vacgathsvg
      241 ltpsgtvfvw grnscgqlgl sdqkdrphpt vikplrsqri khvccgenht aaltadggvf
      301 tfgagthgql ghgvkvnevl prrvvelmgt aitqlgcgrc htvayapqsg klyifglgks
      361 gqlgngvles salpvtipgl whgvahfsqi drppsprpqt srmpaeessp vedtwpsdrs
      421 ealsghdqgt lvvpspaghd errtpddvia tdlamldasd aedtessqss gvssidpdap
      481 lvvykviagf drnfllchen kadaprpvdq rfhlketqil cldndileql pfllateqvp
      541 qellayletv fsscaclnas ylkeddghfn cselnpgvdl eaarhgfqci hdaknpsvae
      601 vvaacleemv kglatgpsdi eamrvllllp lchffrdpsr yletllgkyc lcisrlgara
      661 aeviskwwsl ltqaqfedll aifkecvvyi lsremqtkkn hghlklaldv laklntvnke
      721 cgglvsyehf yipdvtdkvd vqldyihwiq tsredrshkv yfceypfvfn aqaktlilqt
      781 dshlqmqqam esacqqtfas lilpvvppsn pylvlyvgre nivndtlnql adqsaadlkk
      841 plkvqfhged avdaggvkke ffllllkeil dpkygmftey pesrciwfnt qsfeedvmyy
      901 ligivcglai ynftiialpf plvlykkllk qkptlkdlqd lsptlakglq nlldyngedl
      961 entffltfev svehyghtls helkkggnni kvtkenrqey vdlyvdfvlt tsvqhcfeaf
     1021 aqgfykvcss kvldlfhaqe lmvlvvgsen ynwdelekna eykdefhrnh ptvrmfwkvf
     1081 hsldydekkk fllfltgsdr ipilgmseik vvlqpmkvdd shlpvahtcf nlldlpmyss
     1141 eavmreklrl aiqnaqgfgl v
//
LOCUS       XP_077504327            1161 aa            linear   INV 25-NOV-2025
DEFINITION  HECT and RLD domain containing E3 ubiquitin ligase 4 isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077504327
VERSION     XP_077504327.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648201.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1161
                     /product="HECT and RLD domain containing E3 ubiquitin
                     ligase 4 isoform X1"
                     /calculated_mol_wt=128109
     Region          31..375
                     /region_name="ATS1"
                     /note="Alpha-tubulin suppressor ATS1 and related RCC1
                     domain-containing proteins [Cell cycle control, cell
                     division, chromosome partitioning, Cytoskeleton]; COG5184"
                     /db_xref="CDD:444065"
     Region          813..1159
                     /region_name="HECTc"
                     /note="HECT domain; C-terminal catalytic domain of a
                     subclass of Ubiquitin-protein ligase (E3). It binds
                     specific ubiquitin-conjugating enzymes (E2), accepts
                     ubiquitin from E2, transfers ubiquitin to substrate lysine
                     side chains, and transfers additional...; cd00078"
                     /db_xref="CDD:238033"
     Site            order(819,849,860,917,1094,1123..1124,1127..1131,1151,
                     1158)
                     /site_type="active"
                     /note="catalytic cleft [active]"
                     /db_xref="CDD:238033"
     Site            order(945,948..949,951..952,955,962,964,968..969,971,978,
                     983,1000,1004)
                     /site_type="active"
                     /note="E2 interaction site [active]"
                     /db_xref="CDD:238033"
     CDS             1..1161
                     /gene="Herc4"
                     /coded_by="XM_077648201.1:137..3622"
                     /db_xref="GeneID:144114457"
ORIGIN      
        1 mrrshsdtaa ggsgrtspsl gpigpgapmm lyswglgphg elgltqgkdg vdtetavcqp
       61 ervrrfgtra vrevacgqkh tlvllangsv yscgsndfgq lgqdrsmrkl eqvaalaahe
      121 iravacgevh slalsdagqv fswgcnrngq lgcgtedesr lrpkvikkla tlhvvqiacg
      181 svhclalann gelyswggna ygqlglgrds sgderlprrv thlggvplaq vacgathsvg
      241 ltpsgtvfvw grnscgqlgl sdqkdrphpt vikplrsqri khvccgenht aaltadggvf
      301 tfgagthgql ghgvkvnevl prrvvelmgt aitqlgcgrc htvayapqsg klyifglgks
      361 gqlgngvles salpvtipgl whgvahfsqi drppsprpqt srmpaeessp vedtwpsdrs
      421 ealsghdqgt lvvpspaghd errtpddvia tdlamldasd aedtessqss gvssidpdap
      481 lvvykviagf drnfllchen kadaprpvdq rfhlketqil cldndileql pfllateqvp
      541 qellayletv fsscaclnas ylkeddghfn cselnpgvdl eaarhgfqci hdaknpsvae
      601 vvaacleemv kglatgpsdi eamrvllllp lchffrdpsr yletllgkyc lcisrlgara
      661 aeviskwwsl ltqaqfedll aifkecvvyi lsremqtkkn hghlklaldv laklntvnke
      721 cgglvsyehf yipdvtdkvd vqldyihwiq tsredrshkv yfceypfvfn aqaktlilqt
      781 dshlqmqqam esacqqtfas lilpvvppsn pylvlyvgre nivndtlnql adqsaadlkk
      841 plkvqfhged avdaggvkke ffllllkeil dpkygmftey pesrciwfnt qsfeedvmyy
      901 ligivcglai ynftiialpf plvlykkllk qkptlkdlqd lsptlakglq nlldyngedl
      961 entffltfev svehyghtls helkkggnni kvtkenrqey vdlyvdfvlt tsvqhcfeaf
     1021 aqgfykvcss kvldlfhaqe lmvlvvgsen ynwdelekna eykdefhrnh ptvrmfwkvf
     1081 hsldydekkk fllfltgsdr ipilgmseik vvlqpmkvdd shlpvahtcf nlldlpmyss
     1141 eavmreklrl aiqnaqgfgl v
//
LOCUS       XP_077504328            1160 aa            linear   INV 25-NOV-2025
DEFINITION  HECT and RLD domain containing E3 ubiquitin ligase 4 isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077504328
VERSION     XP_077504328.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648202.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1160
                     /product="HECT and RLD domain containing E3 ubiquitin
                     ligase 4 isoform X2"
                     /calculated_mol_wt=127980
     Region          31..375
                     /region_name="ATS1"
                     /note="Alpha-tubulin suppressor ATS1 and related RCC1
                     domain-containing proteins [Cell cycle control, cell
                     division, chromosome partitioning, Cytoskeleton]; COG5184"
                     /db_xref="CDD:444065"
     Region          812..1158
                     /region_name="HECTc"
                     /note="HECT domain; C-terminal catalytic domain of a
                     subclass of Ubiquitin-protein ligase (E3). It binds
                     specific ubiquitin-conjugating enzymes (E2), accepts
                     ubiquitin from E2, transfers ubiquitin to substrate lysine
                     side chains, and transfers additional...; cd00078"
                     /db_xref="CDD:238033"
     Site            order(818,848,859,916,1093,1122..1123,1126..1130,1150,
                     1157)
                     /site_type="active"
                     /note="catalytic cleft [active]"
                     /db_xref="CDD:238033"
     Site            order(944,947..948,950..951,954,961,963,967..968,970,977,
                     982,999,1003)
                     /site_type="active"
                     /note="E2 interaction site [active]"
                     /db_xref="CDD:238033"
     CDS             1..1160
                     /gene="Herc4"
                     /coded_by="XM_077648202.1:219..3701"
                     /db_xref="GeneID:144114457"
ORIGIN      
        1 mrrshsdtaa ggsgrtspsl gpigpgapmm lyswglgphg elgltqgkdg vdtetavcqp
       61 ervrrfgtra vrevacgqkh tlvllangsv yscgsndfgq lgqdrsmrkl eqvaalaahe
      121 iravacgevh slalsdagqv fswgcnrngq lgcgtedesr lrpkvikkla tlhvvqiacg
      181 svhclalann gelyswggna ygqlglgrds sgderlprrv thlggvplaq vacgathsvg
      241 ltpsgtvfvw grnscgqlgl sdqkdrphpt vikplrsqri khvccgenht aaltadggvf
      301 tfgagthgql ghgvkvnevl prrvvelmgt aitqlgcgrc htvayapqsg klyifglgks
      361 gqlgngvles salpvtipgl whgvahfsqi drppsprpqt srmpaesspv edtwpsdrse
      421 alsghdqgtl vvpspaghde rrtpddviat dlamldasda edtessqssg vssidpdapl
      481 vvykviagfd rnfllchenk adaprpvdqr fhlketqilc ldndileqlp fllateqvpq
      541 ellayletvf sscaclnasy lkeddghfnc selnpgvdle aarhgfqcih daknpsvaev
      601 vaacleemvk glatgpsdie amrvllllpl chffrdpsry letllgkycl cisrlgaraa
      661 eviskwwsll tqaqfedlla ifkecvvyil sremqtkknh ghlklaldvl aklntvnkec
      721 gglvsyehfy ipdvtdkvdv qldyihwiqt sredrshkvy fceypfvfna qaktlilqtd
      781 shlqmqqame sacqqtfasl ilpvvppsnp ylvlyvgren ivndtlnqla dqsaadlkkp
      841 lkvqfhgeda vdaggvkkef fllllkeild pkygmfteyp esrciwfntq sfeedvmyyl
      901 igivcglaiy nftiialpfp lvlykkllkq kptlkdlqdl sptlakglqn lldyngedle
      961 ntffltfevs vehyghtlsh elkkggnnik vtkenrqeyv dlyvdfvltt svqhcfeafa
     1021 qgfykvcssk vldlfhaqel mvlvvgseny nwdeleknae ykdefhrnhp tvrmfwkvfh
     1081 sldydekkkf llfltgsdri pilgmseikv vlqpmkvdds hlpvahtcfn lldlpmysse
     1141 avmreklrla iqnaqgfglv
//
LOCUS       XP_077504329            1100 aa            linear   INV 25-NOV-2025
DEFINITION  HECT and RLD domain containing E3 ubiquitin ligase 4 isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077504329
VERSION     XP_077504329.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648203.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1100
                     /product="HECT and RLD domain containing E3 ubiquitin
                     ligase 4 isoform X3"
                     /calculated_mol_wt=121602
     Region          31..375
                     /region_name="ATS1"
                     /note="Alpha-tubulin suppressor ATS1 and related RCC1
                     domain-containing proteins [Cell cycle control, cell
                     division, chromosome partitioning, Cytoskeleton]; COG5184"
                     /db_xref="CDD:444065"
     Region          752..1098
                     /region_name="HECTc"
                     /note="HECT domain; C-terminal catalytic domain of a
                     subclass of Ubiquitin-protein ligase (E3). It binds
                     specific ubiquitin-conjugating enzymes (E2), accepts
                     ubiquitin from E2, transfers ubiquitin to substrate lysine
                     side chains, and transfers additional...; cd00078"
                     /db_xref="CDD:238033"
     Site            order(758,788,799,856,1033,1062..1063,1066..1070,1090,
                     1097)
                     /site_type="active"
                     /note="catalytic cleft [active]"
                     /db_xref="CDD:238033"
     Site            order(884,887..888,890..891,894,901,903,907..908,910,917,
                     922,939,943)
                     /site_type="active"
                     /note="E2 interaction site [active]"
                     /db_xref="CDD:238033"
     CDS             1..1100
                     /gene="Herc4"
                     /coded_by="XM_077648203.1:221..3523"
                     /db_xref="GeneID:144114457"
ORIGIN      
        1 mrrshsdtaa ggsgrtspsl gpigpgapmm lyswglgphg elgltqgkdg vdtetavcqp
       61 ervrrfgtra vrevacgqkh tlvllangsv yscgsndfgq lgqdrsmrkl eqvaalaahe
      121 iravacgevh slalsdagqv fswgcnrngq lgcgtedesr lrpkvikkla tlhvvqiacg
      181 svhclalann gelyswggna ygqlglgrds sgderlprrv thlggvplaq vacgathsvg
      241 ltpsgtvfvw grnscgqlgl sdqkdrphpt vikplrsqri khvccgenht aaltadggvf
      301 tfgagthgql ghgvkvnevl prrvvelmgt aitqlgcgrc htvayapqsg klyifglgks
      361 gqlgngvles salpvtipgl whgvahfsqi drppsprpqt srmpaaqssg vssidpdapl
      421 vvykviagfd rnfllchenk adaprpvdqr fhlketqilc ldndileqlp fllateqvpq
      481 ellayletvf sscaclnasy lkeddghfnc selnpgvdle aarhgfqcih daknpsvaev
      541 vaacleemvk glatgpsdie amrvllllpl chffrdpsry letllgkycl cisrlgaraa
      601 eviskwwsll tqaqfedlla ifkecvvyil sremqtkknh ghlklaldvl aklntvnkec
      661 gglvsyehfy ipdvtdkvdv qldyihwiqt sredrshkvy fceypfvfna qaktlilqtd
      721 shlqmqqame sacqqtfasl ilpvvppsnp ylvlyvgren ivndtlnqla dqsaadlkkp
      781 lkvqfhgeda vdaggvkkef fllllkeild pkygmfteyp esrciwfntq sfeedvmyyl
      841 igivcglaiy nftiialpfp lvlykkllkq kptlkdlqdl sptlakglqn lldyngedle
      901 ntffltfevs vehyghtlsh elkkggnnik vtkenrqeyv dlyvdfvltt svqhcfeafa
      961 qgfykvcssk vldlfhaqel mvlvvgseny nwdeleknae ykdefhrnhp tvrmfwkvfh
     1021 sldydekkkf llfltgsdri pilgmseikv vlqpmkvdds hlpvahtcfn lldlpmysse
     1081 avmreklrla iqnaqgfglv
//
LOCUS       XP_077504330            1100 aa            linear   INV 25-NOV-2025
DEFINITION  HECT and RLD domain containing E3 ubiquitin ligase 4 isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077504330
VERSION     XP_077504330.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648204.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1100
                     /product="HECT and RLD domain containing E3 ubiquitin
                     ligase 4 isoform X3"
                     /calculated_mol_wt=121602
     Region          31..375
                     /region_name="ATS1"
                     /note="Alpha-tubulin suppressor ATS1 and related RCC1
                     domain-containing proteins [Cell cycle control, cell
                     division, chromosome partitioning, Cytoskeleton]; COG5184"
                     /db_xref="CDD:444065"
     Region          752..1098
                     /region_name="HECTc"
                     /note="HECT domain; C-terminal catalytic domain of a
                     subclass of Ubiquitin-protein ligase (E3). It binds
                     specific ubiquitin-conjugating enzymes (E2), accepts
                     ubiquitin from E2, transfers ubiquitin to substrate lysine
                     side chains, and transfers additional...; cd00078"
                     /db_xref="CDD:238033"
     Site            order(758,788,799,856,1033,1062..1063,1066..1070,1090,
                     1097)
                     /site_type="active"
                     /note="catalytic cleft [active]"
                     /db_xref="CDD:238033"
     Site            order(884,887..888,890..891,894,901,903,907..908,910,917,
                     922,939,943)
                     /site_type="active"
                     /note="E2 interaction site [active]"
                     /db_xref="CDD:238033"
     CDS             1..1100
                     /gene="Herc4"
                     /coded_by="XM_077648204.1:130..3432"
                     /db_xref="GeneID:144114457"
ORIGIN      
        1 mrrshsdtaa ggsgrtspsl gpigpgapmm lyswglgphg elgltqgkdg vdtetavcqp
       61 ervrrfgtra vrevacgqkh tlvllangsv yscgsndfgq lgqdrsmrkl eqvaalaahe
      121 iravacgevh slalsdagqv fswgcnrngq lgcgtedesr lrpkvikkla tlhvvqiacg
      181 svhclalann gelyswggna ygqlglgrds sgderlprrv thlggvplaq vacgathsvg
      241 ltpsgtvfvw grnscgqlgl sdqkdrphpt vikplrsqri khvccgenht aaltadggvf
      301 tfgagthgql ghgvkvnevl prrvvelmgt aitqlgcgrc htvayapqsg klyifglgks
      361 gqlgngvles salpvtipgl whgvahfsqi drppsprpqt srmpaaqssg vssidpdapl
      421 vvykviagfd rnfllchenk adaprpvdqr fhlketqilc ldndileqlp fllateqvpq
      481 ellayletvf sscaclnasy lkeddghfnc selnpgvdle aarhgfqcih daknpsvaev
      541 vaacleemvk glatgpsdie amrvllllpl chffrdpsry letllgkycl cisrlgaraa
      601 eviskwwsll tqaqfedlla ifkecvvyil sremqtkknh ghlklaldvl aklntvnkec
      661 gglvsyehfy ipdvtdkvdv qldyihwiqt sredrshkvy fceypfvfna qaktlilqtd
      721 shlqmqqame sacqqtfasl ilpvvppsnp ylvlyvgren ivndtlnqla dqsaadlkkp
      781 lkvqfhgeda vdaggvkkef fllllkeild pkygmfteyp esrciwfntq sfeedvmyyl
      841 igivcglaiy nftiialpfp lvlykkllkq kptlkdlqdl sptlakglqn lldyngedle
      901 ntffltfevs vehyghtlsh elkkggnnik vtkenrqeyv dlyvdfvltt svqhcfeafa
      961 qgfykvcssk vldlfhaqel mvlvvgseny nwdeleknae ykdefhrnhp tvrmfwkvfh
     1021 sldydekkkf llfltgsdri pilgmseikv vlqpmkvdds hlpvahtcfn lldlpmysse
     1081 avmreklrla iqnaqgfglv
//
LOCUS       XP_077504331             185 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114458 [Amblyomma americanum].
ACCESSION   XP_077504331
VERSION     XP_077504331.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648205.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..185
                     /product="uncharacterized protein LOC144114458"
                     /calculated_mol_wt=19459
     CDS             1..185
                     /gene="LOC144114458"
                     /coded_by="XM_077648205.1:226..783"
                     /db_xref="GeneID:144114458"
ORIGIN      
        1 mngcttllav allaacwaqa adpkataatg astaepgege gvegrilgig klrgtlrqlh
       61 nkrpsligtl lrghkyrdgg qsplggahyg hvqggqqhgy qqaaqgfnqg sqafegqdky
      121 knvqgfretk gyrtemgfee tsfnrygsgt gafqggfnqh aagkfqqqgq qqsgfvaapa
      181 pygrr
//
LOCUS       XP_077504332             549 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger CCCH-type with G patch domain-containing protein
            isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504332
VERSION     XP_077504332.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648206.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..549
                     /product="zinc finger CCCH-type with G patch
                     domain-containing protein isoform X1"
                     /calculated_mol_wt=59868
     Region          173..197
                     /region_name="ZnF_C3H1"
                     /note="zinc finger; smart00356"
                     /db_xref="CDD:214632"
     Region          212..267
                     /region_name="Tudor_ZGPAT"
                     /note="Tudor domain found in zinc finger CCCH-type with G
                     patch domain-containing protein (ZGPAT) and similar
                     proteins; cd20384"
                     /db_xref="CDD:410455"
     Site            order(225,230,232,250,253,255)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:410455"
     Region          324..366
                     /region_name="G-patch"
                     /note="G-patch domain; pfam01585"
                     /db_xref="CDD:396249"
     CDS             1..549
                     /gene="LOC144114461"
                     /coded_by="XM_077648206.1:125..1774"
                     /db_xref="GeneID:144114461"
ORIGIN      
        1 mdtdgdkaln daleqyeeql atvelslass vcqsqvaell elkknleevi sltkatlhgv
       61 edetstagqe qvrndsseni deefakfqae maslgassep qaaavstaed hskelaelvd
      121 hlegskvrap fskewgqmsy hsaivlsvea sdimhiddik vnvmfthpta pamrpcsffl
      181 dgrckfsqer crfshghava lsdireysep sfsnlkpgsp clvrveadgl wqlaamecig
      241 qepdkivvrl shsgktasvs vedvfplddd gaeessdvdd gsalaaepls itggcedeed
      301 dvpvvvwsas taaglplgaw ekhtkgigsk lmekmgyvwg qglgirgngr tepveavvlp
      361 agksldkcme lkelgmeqds akvqrkmllk mkreeekier ryhkaakses vfdflngqif
      421 vkqtaksiga qgnlqkeknd sealksvsfr slgiealqvt eaikkaqrei irlghssarn
      481 kdriraqqas plftalkgrc ltvvipcfty tqdssvlhpg dnlrigrvsl llsippfgss
      541 wtlllcmhv
//
LOCUS       XP_077504333             528 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger CCCH-type with G patch domain-containing protein
            isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504333
VERSION     XP_077504333.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648207.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..528
                     /product="zinc finger CCCH-type with G patch
                     domain-containing protein isoform X2"
                     /calculated_mol_wt=58051
     Region          173..197
                     /region_name="ZnF_C3H1"
                     /note="zinc finger; smart00356"
                     /db_xref="CDD:214632"
     Region          212..267
                     /region_name="Tudor_ZGPAT"
                     /note="Tudor domain found in zinc finger CCCH-type with G
                     patch domain-containing protein (ZGPAT) and similar
                     proteins; cd20384"
                     /db_xref="CDD:410455"
     Site            order(225,230,232,250,253,255)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:410455"
     Region          324..366
                     /region_name="G-patch"
                     /note="G-patch domain; pfam01585"
                     /db_xref="CDD:396249"
     CDS             1..528
                     /gene="LOC144114461"
                     /coded_by="XM_077648207.1:123..1709"
                     /db_xref="GeneID:144114461"
ORIGIN      
        1 mdtdgdkaln daleqyeeql atvelslass vcqsqvaell elkknleevi sltkatlhgv
       61 edetstagqe qvrndsseni deefakfqae maslgassep qaaavstaed hskelaelvd
      121 hlegskvrap fskewgqmsy hsaivlsvea sdimhiddik vnvmfthpta pamrpcsffl
      181 dgrckfsqer crfshghava lsdireysep sfsnlkpgsp clvrveadgl wqlaamecig
      241 qepdkivvrl shsgktasvs vedvfplddd gaeessdvdd gsalaaepls itggcedeed
      301 dvpvvvwsas taaglplgaw ekhtkgigsk lmekmgyvwg qglgirgngr tepveavvlp
      361 agksldkcme lkelgmeqds akvqrkmllk mkreeekier ryhkaakses vfdflngqif
      421 vkqtaksiga qgnlqkeknd sealksvsfr slgiealqvt eaikkaqrei irlghssarn
      481 kdrdkvmhaq vekkieeqrq lirhlqskeq siaaeqqhrk gndklrif
//
LOCUS       XP_077504334             128 aa            linear   INV 25-NOV-2025
DEFINITION  histone H2A.V-like [Amblyomma americanum].
ACCESSION   XP_077504334
VERSION     XP_077504334.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648208.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..128
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..128
                     /product="histone H2A.V-like"
                     /calculated_mol_wt=13381
     Region          3..127
                     /region_name="PTZ00017"
                     /note="histone H2A; Provisional"
                     /db_xref="CDD:185399"
     CDS             1..128
                     /gene="LOC144114462"
                     /coded_by="XM_077648208.1:128..514"
                     /db_xref="GeneID:144114462"
ORIGIN      
        1 maggkagkds gkakakavsr saraglqfpv grihrylknr itghrrvgat aavysaaile
       61 yltaevlela gnvskdtkmk sitprhlqla igsdeeldsf ikatiagggv iphihkalig
      121 kkgsqkkp
//
LOCUS       XP_077504336             887 aa            linear   INV 25-NOV-2025
DEFINITION  microsomal triacylglycerol transfer protein [Amblyomma americanum].
ACCESSION   XP_077504336
VERSION     XP_077504336.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648210.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..887
                     /product="microsomal triacylglycerol transfer protein"
                     /calculated_mol_wt=97110
     Region          25..575
                     /region_name="Vitellogenin_N"
                     /note="Lipoprotein amino terminal region; pfam01347"
                     /db_xref="CDD:460170"
     Region          593..885
                     /region_name="Vit_open_b-sht"
                     /note="Vitellinogen, open beta-sheet; cl11721"
                     /db_xref="CDD:472237"
     CDS             1..887
                     /gene="Mtp"
                     /coded_by="XM_077648210.1:556..3219"
                     /db_xref="GeneID:144114463"
ORIGIN      
        1 martrlllpw llaaiaclaa psrqfelgtt ykyglqsdvf lneaaprpaa ktsksvgygl
       61 raaaavtclw kspqdplnrl frielsqvhl tvvpadspes grsehgsqwd aasplplyvl
      121 qqsneivkvw shkesptsvn ikkavasmlq yqltpedkqq vdvsgrcsvs fkterntisk
      181 hktlcthpdk tqyfypnhiq sptvltksts lyellpdmta pksvqiseqt qmyvnilkea
      241 silvkaehsy klestspsgq tftaateaev iqkltagqkl tedtivarpe pkkctkncks
      301 laenvklfes nlraknlatl ksalsflrll esfreaseke vhavlksskn kniapqlldl
      361 aaatqtvasv kaalkflnfn segdlaerfl vgllaashps paiiteilgv cqkdirnqkn
      421 kasavaalga lvnvycrghr dcekteavga alkfledklr qckeepcwqm hlrglqnaaq
      481 pstlptllya vrkggkkavl salraikqvg nehfndqvld alqkvygqvd rrhdstaral
      541 aaelifssts vrhiertlel lprveapela tfvasklfel vdradsgrsf agkvlsnstf
      601 gnyynlahsg ssatftkylt kgedlnstya vnmelipggl lkessldvnl etpderlnli
      661 svglfaggld glaggddsgg aggeeeeata gmrlsllgye lrpyvffvgt selmshvwsg
      721 taseptpalq gnlllmdhyq fvallnglvl elklqgvisl dmtgsiqisl wnrnshsvvr
      781 tsgatvvqas aslnsdaass hvqlnvagdt hlefvtdldf yekpykmciq mtqpgvvlrh
      841 nirkyesvtg rkhlvrrlkr rsqnisgksy afhkknceyc svlladv
//
LOCUS       XP_077504337            1768 aa            linear   INV 25-NOV-2025
DEFINITION  MAGUK family member discs large 5 [Amblyomma americanum].
ACCESSION   XP_077504337
VERSION     XP_077504337.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648211.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1768
                     /product="MAGUK family member discs large 5"
                     /calculated_mol_wt=196548
     Region          131..>483
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          447..>524
                     /region_name="DR0291"
                     /note="Predicted nucleic acid-binding protein DR0291,
                     contains C4-type Zn-ribbon domain [General function
                     prediction only]; COG1579"
                     /db_xref="CDD:441187"
     Region          537..628
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cl49608"
                     /db_xref="CDD:483948"
     Site            order(552..558,576,579,607,611,614..615)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467153"
     Region          641..711
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cl49608"
                     /db_xref="CDD:483948"
     Site            order(648..654,660,663,694,698..699)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467153"
     Region          <903..1207
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1193..1274
                     /region_name="PDZ3_DLG5-like"
                     /note="PDZ domain 3 of Discs Large 5 (Dlg5) and related
                     domains; cd06767"
                     /db_xref="CDD:467248"
     Site            order(1206..1212,1222,1225,1254,1258,1261..1262)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467248"
     Region          1345..1425
                     /region_name="PDZ4_DLG5-like"
                     /note="PDZ domain 4 of Discs Large 5 (Dlg5) and related
                     domains; cd06766"
                     /db_xref="CDD:467247"
     Site            order(1357..1363,1373,1376,1404,1408,1411..1412)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467247"
     Region          1443..1504
                     /region_name="SH3_DLG5"
                     /note="Src homology 3 domain of Disks Large homolog 5;
                     cd11860"
                     /db_xref="CDD:212794"
     Site            order(1445,1461..1462)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212794"
     Site            order(1448,1450,1453,1456,1478..1479,1497,1499..1500)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212794"
     Region          1594..1757
                     /region_name="NK"
                     /note="Nucleoside/nucleotide kinase (NK) is a protein
                     superfamily consisting of multiple families of enzymes
                     that share structural similarity and are functionally
                     related to the catalysis of the reversible phosphate group
                     transfer from nucleoside triphosphates...; cl17190"
                     /db_xref="CDD:450170"
     CDS             1..1768
                     /gene="Dlg5"
                     /coded_by="XM_077648211.1:444..5750"
                     /db_xref="GeneID:144114464"
ORIGIN      
        1 mfhggagkns ggteeppgms qsvnlghgka dcllssdrdl prrdlrqvha dqdsvyrrlt
       61 magdrfnpsm gkthasssdw slsscrdydy phlvatdlpv kleqdfyhgk hkskaedhwq
      121 eallmkkeng dllaekrlle qevkrlqriv dekqkelsdl hhhqqeglme tggeaayrqk
      181 ckalkeeydt lhkryadlta ahsahmarle lcqeelqrlk qgaeealaer naalrdrsgl
      241 qqqctaairq wdsalrerne ardqlakvqq qrdeamkein qamavrikas kdlsrlteer
      301 naavqeytli mserdsvhke ierlqeelqe aqnkarqaea ahkasldeme glkreiasal
      361 qdrdralkel sdlrekqaec sqqsgsedas rgevvrkdsl pssladaeqt nqemenlrrn
      421 merlqvelae aqqeaevckr rrdwafserd kivleresir slcdklrrer dravsdlaea
      481 lrdsddikrq rneaskelke lkekieaqle kesrmkqlns vgnnhsrdsa idtdlqewet
      541 evkevhfrye gdfgfdisgg kedmqaepsv ayfyvssilk ggtaegkllv ndiilkvngl
      601 dvagmdkrtl cdtvqnskgs ltlvvkrrra mssrgvimls lnlssshehg itlenglyit
      661 rigpgsvaar egtlavgdri lsingkpved lrkamecldn adspiqlqvv knavfsssps
      721 sstasghnfs eklersplvh gsppshlree ksfvscsvqt dallelgnha ktmtlnsrss
      781 ksggtserat vsvqptlldk aynkifgdkr vskekkallp dekgpieeld smlecysktg
      841 gkrasgrqqq ehnggtwpky rgpplqlgea gvantlqphk rrerkslasf tkqlqvpsya
      901 dfpaelssps sgnctsssgs yaspgsppka psgvqqsser vplhyaaglp edrldysvvs
      961 ahkdvlemyq hrgglksrph sahypreaya kpvapyadvl lsghvhqslr anvplypqpr
     1021 ghqhtyypty isrsgdslla adgaeslryg plsgakgdgr ytpspcpsqy sfsgtspths
     1081 ldfpqqfqpv apapkrlvpt hvhgpfraee typqpsydgl atfpkrnqri ripstpsvts
     1141 kssagkvsts siekapsltd rsspmpaftv elltpngsra itelrgrrrp kpgdmrrivi
     1201 eksleplgiq ikcgaggtif vssvsensia aqaslqvgdq llevcginmr natyqlaanv
     1261 lrqcrdsitm lvqfnpdkyr evasdnists eenspvdtpa aspkmrhskr sdlirisndd
     1321 vpssatstlt rqkqscvsak gsraeprivl lrkaspslgi tllggnavgi fvhsvqedsp
     1381 asgpagllpg dqileyngtd lrnataeeaa yelasntenv hiiaqynper faeiqeqpgd
     1441 sfyvralfdr asidgslafh kddilyvdnt mykgvpgqwr awvldqdgqk lkcgfipsky
     1501 kaeeellmkr slldleeggt grrgststrr sffrrrrhqq rsredgkela sfsdvsinns
     1561 cggsagtlae dvpptylrvd rlnypslrpv ilvgplqeav vdkleqdfpq qfqrcpsqpl
     1621 rgslqaleqg lqdlslldfr rrgshyeclt ldalrhctnh klchalldvs lsaverlnrc
     1681 qiypivifik fkttkqirev kdstyltekv ttkaakemye halkieseyk hlinaiipgs
     1741 nlaymctqvk mcvddeqnka lwvpsgsi
//
LOCUS       XP_077504338             524 aa            linear   INV 25-NOV-2025
DEFINITION  synaptotagmin-15-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504338
VERSION     XP_077504338.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648212.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..524
                     /product="synaptotagmin-15-like isoform X1"
                     /calculated_mol_wt=59140
     Region          <38..>114
                     /region_name="CynX"
                     /note="Cyanate permease [Inorganic ion transport and
                     metabolism]; COG2807"
                     /db_xref="CDD:442057"
     Region          245..367
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          375..513
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     CDS             1..524
                     /gene="LOC144114465"
                     /coded_by="XM_077648212.1:179..1753"
                     /db_xref="GeneID:144114465"
ORIGIN      
        1 mrrlmslspq rlrsddasas avwnvvergw lqlyenpltl taaprltdal gvyvwaiaag
       61 ivtgavllal ivwlvlfrlr gakrkgdsae pplltrepwh rksmlsqpla gvlttkaggl
      121 drgsppsllt pesfdvqpaq ltvaepvqpl arpgfvrwns fcspsaeglv gnllpkgcnv
      181 gspdetrtyq fwvqhgfcrs tryrsclfvh lytpaldkld gtqkqclrys wsddedstvp
      241 rctngrlwfs lnykpeseel dvhivkakyl pgrgltntpr dpfvrayllp dentfqqssv
      301 ktrtlapkfd etltfkises evrtrslrls vydidrkkvr hclghvalql ekepltpdkv
      361 iwldldnvpk kssqigeiqi cmtcnpftnr iraailkvrk fskpddadkw vyvrlslnhg
      421 rkcvkekrse shewgsgdea sfsdaftfgv agryfdscsl sftvmvspdg adyrrlgktv
      481 lgpfmyarge elrhwqdmlt nprsavtrwh alecptgrde wksd
//
LOCUS       XP_077504339             523 aa            linear   INV 25-NOV-2025
DEFINITION  synaptotagmin-15-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504339
VERSION     XP_077504339.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648213.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..523
                     /product="synaptotagmin-15-like isoform X2"
                     /calculated_mol_wt=59053
     Region          <38..>114
                     /region_name="CynX"
                     /note="Cyanate permease [Inorganic ion transport and
                     metabolism]; COG2807"
                     /db_xref="CDD:442057"
     Region          244..366
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          374..512
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     CDS             1..523
                     /gene="LOC144114465"
                     /coded_by="XM_077648213.1:179..1750"
                     /db_xref="GeneID:144114465"
ORIGIN      
        1 mrrlmslspq rlrsddasas avwnvvergw lqlyenpltl taaprltdal gvyvwaiaag
       61 ivtgavllal ivwlvlfrlr gakrkgdsae pplltrepwh rksmlsqpla gvlttkaggl
      121 drgsppsllt pesfdvqpaq ltvaepvqpl arpgfvrwns fcspsaeglv gnllpkgcnv
      181 gpdetrtyqf wvqhgfcrst ryrsclfvhl ytpaldkldg tqkqclrysw sddedstvpr
      241 ctngrlwfsl nykpeseeld vhivkakylp grgltntprd pfvrayllpd entfqqssvk
      301 trtlapkfde tltfkisese vrtrslrlsv ydidrkkvrh clghvalqle kepltpdkvi
      361 wldldnvpkk ssqigeiqic mtcnpftnri raailkvrkf skpddadkwv yvrlslnhgr
      421 kcvkekrses hewgsgdeas fsdaftfgva gryfdscsls ftvmvspdga dyrrlgktvl
      481 gpfmyargee lrhwqdmltn prsavtrwha lecptgrdew ksd
//
LOCUS       XP_077504340             477 aa            linear   INV 25-NOV-2025
DEFINITION  synaptotagmin-15-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077504340
VERSION     XP_077504340.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648214.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..477
                     /product="synaptotagmin-15-like isoform X3"
                     /calculated_mol_wt=53847
     Region          198..320
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          328..466
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     CDS             1..477
                     /gene="LOC144114465"
                     /coded_by="XM_077648214.1:216..1649"
                     /db_xref="GeneID:144114465"
ORIGIN      
        1 mmpgvyvwai aagivtgavl lalivwlvlf rlrgakrkgd saepplltre pwhrksmlsq
       61 plagvlttka ggldrgspps lltpesfdvq paqltvaepv qplarpgfvr wnsfcspsae
      121 glvgnllpkg cnvgspdetr tyqfwvqhgf crstryrscl fvhlytpald kldgtqkqcl
      181 ryswsddeds tvprctngrl wfslnykpes eeldvhivka kylpgrgltn tprdpfvray
      241 llpdentfqq ssvktrtlap kfdetltfki sesevrtrsl rlsvydidrk kvrhclghva
      301 lqlekepltp dkviwldldn vpkkssqige iqicmtcnpf tnriraailk vrkfskpdda
      361 dkwvyvrlsl nhgrkcvkek rseshewgsg deasfsdaft fgvagryfds cslsftvmvs
      421 pdgadyrrlg ktvlgpfmya rgeelrhwqd mltnprsavt rwhalecptg rdewksd
//
LOCUS       XP_077504341             477 aa            linear   INV 25-NOV-2025
DEFINITION  synaptotagmin-15-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077504341
VERSION     XP_077504341.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648215.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..477
                     /product="synaptotagmin-15-like isoform X4"
                     /calculated_mol_wt=53504
     Region          <38..>114
                     /region_name="CynX"
                     /note="Cyanate permease [Inorganic ion transport and
                     metabolism]; COG2807"
                     /db_xref="CDD:442057"
     Region          198..320
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          328..466
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     CDS             1..477
                     /gene="LOC144114465"
                     /coded_by="XM_077648215.1:173..1606"
                     /db_xref="GeneID:144114465"
ORIGIN      
        1 mrrlmslspq rlrsddasas avwnvvergw lqlyenpltl taaprltdal gvyvwaiaag
       61 ivtgavllal ivwlvlfrlr gakrkgdsae pplltrepwh rksmlsqpla gvlttkaggl
      121 drgsppsllt pesfdvqpaq ltvaepvqpl arpgfvrwns fcspsaeglv gnllpkgcnv
      181 gyswsddeds tvprctngrl wfslnykpes eeldvhivka kylpgrgltn tprdpfvray
      241 llpdentfqq ssvktrtlap kfdetltfki sesevrtrsl rlsvydidrk kvrhclghva
      301 lqlekepltp dkviwldldn vpkkssqige iqicmtcnpf tnriraailk vrkfskpdda
      361 dkwvyvrlsl nhgrkcvkek rseshewgsg deasfsdaft fgvagryfds cslsftvmvs
      421 pdgadyrrlg ktvlgpfmya rgeelrhwqd mltnprsavt rwhalecptg rdewksd
//
LOCUS       XP_077504342             430 aa            linear   INV 25-NOV-2025
DEFINITION  synaptotagmin-15-like isoform X5 [Amblyomma americanum].
ACCESSION   XP_077504342
VERSION     XP_077504342.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648216.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..430
                     /product="synaptotagmin-15-like isoform X5"
                     /calculated_mol_wt=48211
     Region          151..273
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          281..419
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     CDS             1..430
                     /gene="LOC144114465"
                     /coded_by="XM_077648216.1:273..1565"
                     /db_xref="GeneID:144114465"
ORIGIN      
        1 mmpgvyvwai aagivtgavl lalivwlvlf rlrgakrkgd saepplltre pwhrksmlsq
       61 plagvlttka ggldrgspps lltpesfdvq paqltvaepv qplarpgfvr wnsfcspsae
      121 glvgnllpkg cnvgyswsdd edstvprctn grlwfslnyk peseeldvhi vkakylpgrg
      181 ltntprdpfv rayllpdent fqqssvktrt lapkfdetlt fkisesevrt rslrlsvydi
      241 drkkvrhclg hvalqlekep ltpdkviwld ldnvpkkssq igeiqicmtc npftnriraa
      301 ilkvrkfskp ddadkwvyvr lslnhgrkcv kekrseshew gsgdeasfsd aftfgvagry
      361 fdscslsftv mvspdgadyr rlgktvlgpf myargeelrh wqdmltnprs avtrwhalec
      421 ptgrdewksd
//
LOCUS       XP_077504343             880 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: N-deacetylase and N-sulfotransferase sfl
            [Amblyomma americanum].
ACCESSION   XP_077504343
VERSION     XP_077504343.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648217.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..880
                     /product="LOW QUALITY PROTEIN: N-deacetylase and
                     N-sulfotransferase sfl"
                     /calculated_mol_wt=101247
     Region          90..513
                     /region_name="HSNSD"
                     /note="heparan sulfate-N-deacetylase; pfam12062"
                     /db_xref="CDD:463450"
     Region          602..>798
                     /region_name="Sulfotransfer_1"
                     /note="Sulfotransferase domain; pfam00685"
                     /db_xref="CDD:425820"
     CDS             1..880
                     /gene="sfl"
                     /coded_by="XM_077648217.1:380..3022"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:144114466"
ORIGIN      
        1 mlleslsavs pwchasqlsi rrcmlgaval glcslllsvy vinhnslrgl lrsplpprpt
       61 fqcrsqldgs gpqeqsaanv hassaqlrld prvlvfvetq fsklgkqice ilegsrfrfk
      121 vettgktlpv ltnldkgkfa vivfenfeky lamhkwnrel idkycreysv giigfirpre
      181 dtytgaqlsg lplymhtnma lkdyqlnpls silriarage tfsgnlpged wtvfqpnhst
      241 fvplaqarsq hddiadgngp httvvqdigk fdgiqrvifg ngfgfwlhkl lfldaisfls
      301 hgklslplqr yllididdif vaergsrmmt hdvqalleaq qqfrklihgf rfnlgfsgkf
      361 yhrgsheens gddmllshah efwwfchmws hsqphlydnv tlletemrln kefakkhriq
      421 tdsgysvaph hsgvypihep lydawkkvwn irvtsteeyp hlrparlrrg fvhrnimvlp
      481 rqtcglytht iflerypggr ekldrsihgg dlfylfvynp inifmthlsn ygndrlslyt
      541 fesvlkfikc wtnlklstip plqlaekyfq mypdetdpiw mnpcedkrhl siwsssksce
      601 qlpkflvigp qktgttalyt fltlhptias nhpspdtfee vqffngknyy kgldwyqsff
      661 papkngsapf lfeksanyfd gelvpqraha llpraklvti lisptkrays wyqhqrshgd
      721 avalnysfyd vitandhspk qlrelrnrcl npglyaqhle rwlsfyppqq lmiidgeelk
      781 sdpvvsinkl qtflaitpff dysgslrfdp hkgffckvls qsnrtkclgq gkgrlyppmd
      841 iraekflkay ylshnvalsk lfnnlrqpqp hwlkedlsrg
//
LOCUS       XP_077504344             406 aa            linear   INV 25-NOV-2025
DEFINITION  S-adenosylmethionine synthase-like [Amblyomma americanum].
ACCESSION   XP_077504344
VERSION     XP_077504344.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648218.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..406
                     /product="S-adenosylmethionine synthase-like"
                     /calculated_mol_wt=44711
     Region          30..402
                     /region_name="S-AdoMet_synt"
                     /note="S-adenosylmethionine synthetase; cd18079"
                     /db_xref="CDD:350837"
     Site            order(30..32,34,68,70,72,77,79..81,145..148,150,192,194,
                     196,213,215,254,257,268..270,272..275,278,283..290,292,
                     296,328,330..334,339..340,342)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:350837"
     Site            order(34,40..42,66,81,124,127..128,144..145,190,192,217,
                     258,260..261,263,269,275..276,290..292,296,300,302,333)
                     /site_type="active"
                     /db_xref="CDD:350837"
     Site            order(34,40..41,190,192,276)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350837"
     CDS             1..406
                     /gene="LOC144114468"
                     /coded_by="XM_077648218.1:125..1345"
                     /db_xref="GeneID:144114468"
ORIGIN      
        1 mgtlqgsnal ngvryvdgqq empgeeetfl ftsesvgegh pdkicdlisd avldaylakd
       61 peakvacesv aktgmillag eitschtidy qrvvrdtikh igyddsskgf dyktcnvvvt
      121 ieqqaqeiar gvhhnreedd lgagdqglmf gyatdetpec mpltvvlahk lnerlaelrr
      181 ngtfwwarpd sksqvtceya frhgatvpkr vhtivvstqh sgevsleeir kqvmeqvirv
      241 vvperyldde tvfhinpcgs fteggpmgda gltgrkiivd tyggwgahgg gafsgkdptk
      301 vdrsaayaar wvakslvvag lckrclvqls yaigiaqpvs isiftygtgt ktprellhiv
      361 kenfdlrpgk iikdlklkep iyrktsaygh fgrseftweq pkklil
//
LOCUS       XP_077504345             810 aa            linear   INV 25-NOV-2025
DEFINITION  salivary peroxidase/catechol oxidase-like [Amblyomma americanum].
ACCESSION   XP_077504345
VERSION     XP_077504345.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648219.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..810
                     /product="salivary peroxidase/catechol oxidase-like"
                     /calculated_mol_wt=90343
     Region          61..101
                     /region_name="CLIP"
                     /note="Regulatory CLIP domain of proteinases; cl02731"
                     /db_xref="CDD:470660"
     Region          238..778
                     /region_name="An_peroxidase"
                     /note="Animal haem peroxidase; pfam03098"
                     /db_xref="CDD:460804"
     CDS             1..810
                     /gene="LOC144114469"
                     /coded_by="XM_077648219.1:222..2654"
                     /db_xref="GeneID:144114469"
ORIGIN      
        1 mrtllftaal allllagpsn aqrrrgqgqg rgqsrgrgqp sqsqsqsqrq tapavrpnsd
       61 crtasgqpgv cqalshcvfq fnviediass rcgdairvcc plntpaasgq spvsrpvtvi
      121 grrvavpkis kddldiagra slsvvrgied aerallndgl fakrhttasl haaffgskpi
      181 vqslgrdgli gleatrelar rfnldstqgr dglqrfniqn tviadacpki prcastkyrs
      241 adglcnnlqn rewgksltaf erfmppsyad gltlprvgvd gnelpnprki svaadpdadf
      301 pnerntlmlm qfaqfvdhdl tltgvsrfrn gsaitccdee liqnptkrhy acmpidlpad
      361 dhfyaqfrlr clefvrsvpa prpkctfgpr eqlnqltaym dasniygste eeakalrsfr
      421 dgrlastsfs rdellprqtd stqecneqgt dficfragde rvneqlslta mhtlwlrehn
      481 rvaaelhrln pgwkdeilyq earrivaaef qhiafneflp illgrnvmeq fdllltpygy
      541 shsydpelna gignvfaaaa yryghtlvqg nielinedgs vhkrvplatq ffnpaeiyap
      601 gnldrfyrgl isqpaqtydr fvteqltnhl yeplgqgfgm dlvalniqrg rdhgipgynd
      661 wreycgmsri tdfsqlaeim tpesaqafaq vykspddidl fpagvnersv pggtlgptfa
      721 civaeqfrrm kngdrfwyen gglessfnev qieeirkasl ariicdntnl qyvqplvmir
      781 elswnpkvdc ngadiprvdl dnwqnepvwt
//
LOCUS       XP_077504347             411 aa            linear   INV 25-NOV-2025
DEFINITION  S-adenosylmethionine synthase-like [Amblyomma americanum].
ACCESSION   XP_077504347
VERSION     XP_077504347.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648221.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..411
                     /product="S-adenosylmethionine synthase-like"
                     /calculated_mol_wt=45366
     Region          32..411
                     /region_name="PTZ00104"
                     /note="S-adenosylmethionine synthase; Provisional"
                     /db_xref="CDD:240268"
     Site            order(35..37,39,73,75,77,82,84..86,150..153,155,197,199,
                     201,218,220,259,262,273..275,277..280,283,288..295,297,
                     301,333,335..339,344..345,347)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:350837"
     Site            order(39,45..47,71,86,129,132..133,149..150,195,197,222,
                     263,265..266,268,274,280..281,295..297,301,305,307,338)
                     /site_type="active"
                     /db_xref="CDD:350837"
     Site            order(39,45..46,195,197,281)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350837"
     CDS             1..411
                     /gene="LOC144114471"
                     /coded_by="XM_077648221.1:69..1304"
                     /db_xref="GeneID:144114471"
ORIGIN      
        1 malinngnhv ltngealhdv hdneelgdds letflfsses vgeghtdkmc dqisdailda
       61 hlrqdpnakv acetatktgm imvfgeitsd avvdyqrivr etvkkigydc stkgfdyrtc
      121 nvlvaieqqs peiaagvhqq rkeedigagd qglmfgyatd etdecmpltv vlahklnkrl
      181 aelrrdgtfp wagpdsktqv tceyyfdhgk avpirvhtvv vstqhsedis leelrkevme
      241 hvvkyvipak yldrrtvyhi npcgkfvqag pmgdagltgr kiivdtyggw gahgggafsg
      301 kdptkvdrsa ayaarwvaks lvkagickrc lvqvsyaigi aeplsitvlp ygtsqysqke
      361 lleivnknfd lrpgiiirdl qlknpiyqst sayghfgrke fpwevpkqlv l
//
LOCUS       XP_077504348             915 aa            linear   INV 25-NOV-2025
DEFINITION  golgin subfamily A member 2-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504348
VERSION     XP_077504348.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648222.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..915
                     /product="golgin subfamily A member 2-like isoform X1"
                     /calculated_mol_wt=103240
     Region          <156..>317
                     /region_name="COG2433"
                     /note="Possible nuclease of RNase H fold, RuvC/YqgF family
                     [General function prediction only]"
                     /db_xref="CDD:441980"
     Region          374..826
                     /region_name="GOLGA2L5"
                     /note="Putative golgin subfamily A member 2-like protein
                     5; pfam15070"
                     /db_xref="CDD:464485"
     CDS             1..915
                     /gene="LOC144114473"
                     /coded_by="XM_077648222.1:1..2748"
                     /db_xref="GeneID:144114473"
ORIGIN      
        1 mrmsavsanm addvdrkhkv aaglkklkqf qqrsvkrttk rrssdtvspq ivqedqtsaq
       61 pldeqssssm sqpskpkeal avtshscqdl sqltddiqps qrvlssaesl rqitmqlngl
      121 medtsaalng ematladsss galrsadall rrnqelatal ealqqdrdql efqlqelkgk
      181 larqqrefdr ereekeeqgr reqgalndql qvhiqtigil vaektelqsa lsqsqqtakq
      241 kaveaeelqg rlkalrerns dlerklstas nqsqvqekst kesqrevdrl ktenyrlsks
      301 veelrqelae lsdklsrksk eaeegqqela kvrqelslaq lyaqqlggsq aseealgrme
      361 qlhqeklhfe rraaeykdam eqiaaekqqm ashyqsyvds vsqqletykt klaemseike
      421 slekklqqll dkpvppavqv cedpeykakv eqceqelasl katcetqatd nrqlsdllae
      481 reaqaeelaa alerlredhv essrlleaiq sekvaasral sqnrelkrql eemqdvfvkl
      541 sndklelteq lqkeqhvtke lgerlgqqee elrelrdqla skegqvqgle qlssrehyqq
      601 sqladrvrhy qaqcqlteil qqelaqaqvc cartnalvtq nsdlrkalae kaqiavdvqe
      661 kdsskvhdlv aslsasvqql elerdqlvhq ldeqrtqres lqdqlfefkk teevllsheq
      721 eevtregyak lkramqqled rfkatmeqia nlsdqkqrle hlvtqlqget dtiadyialy
      781 qvqrgimrkr aaekddyisq lakdredlka klaelqcliv rlleerqqqs gqsalplpgk
      841 vltvssftda lqngpsagdt gsapctdsad evqdataqki mhllteiess savdgspvpd
      901 tfhpcpvcsg rllti
//
LOCUS       XP_077504349             914 aa            linear   INV 25-NOV-2025
DEFINITION  golgin subfamily A member 2-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504349
VERSION     XP_077504349.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648223.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..914
                     /product="golgin subfamily A member 2-like isoform X2"
                     /calculated_mol_wt=103153
     Region          <155..>316
                     /region_name="COG2433"
                     /note="Possible nuclease of RNase H fold, RuvC/YqgF family
                     [General function prediction only]"
                     /db_xref="CDD:441980"
     Region          373..825
                     /region_name="GOLGA2L5"
                     /note="Putative golgin subfamily A member 2-like protein
                     5; pfam15070"
                     /db_xref="CDD:464485"
     CDS             1..914
                     /gene="LOC144114473"
                     /coded_by="XM_077648223.1:1..2745"
                     /db_xref="GeneID:144114473"
ORIGIN      
        1 mrmsavsanm addvdrkhkv aaglkklkqf qqrsvkrttk rrssdtvspq ivqedqtsaq
       61 pldeqsssms qpskpkeala vtshscqdls qltddiqpsq rvlssaeslr qitmqlnglm
      121 edtsaalnge matladsssg alrsadallr rnqelatale alqqdrdqle fqlqelkgkl
      181 arqqrefdre reekeeqgrr eqgalndqlq vhiqtigilv aektelqsal sqsqqtakqk
      241 aveaeelqgr lkalrernsd lerklstasn qsqvqekstk esqrevdrlk tenyrlsksv
      301 eelrqelael sdklsrkske aeegqqelak vrqelslaql yaqqlggsqa seealgrmeq
      361 lhqeklhfer raaeykdame qiaaekqqma shyqsyvdsv sqqletyktk laemseikes
      421 lekklqqlld kpvppavqvc edpeykakve qceqelaslk atcetqatdn rqlsdllaer
      481 eaqaeelaaa lerlredhve ssrlleaiqs ekvaasrals qnrelkrqle emqdvfvkls
      541 ndklelteql qkeqhvtkel gerlgqqeee lrelrdqlas kegqvqgleq lssrehyqqs
      601 qladrvrhyq aqcqlteilq qelaqaqvcc artnalvtqn sdlrkalaek aqiavdvqek
      661 dsskvhdlva slsasvqqle lerdqlvhql deqrtqresl qdqlfefkkt eevllsheqe
      721 evtregyakl kramqqledr fkatmeqian lsdqkqrleh lvtqlqgetd tiadyialyq
      781 vqrgimrkra aekddyisql akdredlkak laelqclivr lleerqqqsg qsalplpgkv
      841 ltvssftdal qngpsagdtg sapctdsade vqdataqkim hllteiesss avdgspvpdt
      901 fhpcpvcsgr llti
//
LOCUS       XP_077504350             912 aa            linear   INV 25-NOV-2025
DEFINITION  golgin subfamily A member 2-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077504350
VERSION     XP_077504350.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648224.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..912
                     /product="golgin subfamily A member 2-like isoform X3"
                     /calculated_mol_wt=102934
     Region          <156..>317
                     /region_name="COG2433"
                     /note="Possible nuclease of RNase H fold, RuvC/YqgF family
                     [General function prediction only]"
                     /db_xref="CDD:441980"
     Region          374..823
                     /region_name="GOLGA2L5"
                     /note="Putative golgin subfamily A member 2-like protein
                     5; pfam15070"
                     /db_xref="CDD:464485"
     CDS             1..912
                     /gene="LOC144114473"
                     /coded_by="XM_077648224.1:1..2739"
                     /db_xref="GeneID:144114473"
ORIGIN      
        1 mrmsavsanm addvdrkhkv aaglkklkqf qqrsvkrttk rrssdtvspq ivqedqtsaq
       61 pldeqssssm sqpskpkeal avtshscqdl sqltddiqps qrvlssaesl rqitmqlngl
      121 medtsaalng ematladsss galrsadall rrnqelatal ealqqdrdql efqlqelkgk
      181 larqqrefdr ereekeeqgr reqgalndql qvhiqtigil vaektelqsa lsqsqqtakq
      241 kaveaeelqg rlkalrerns dlerklstas nqsqvqekst kesqrevdrl ktenyrlsks
      301 veelrqelae lsdklsrksk eaeegqqela kvrqelslaq lyaqqlggsq aseealgrme
      361 qlhqeklhfe rraaeykdam eqiaaekqqm ashyqsyvds vsqqletykt klaemseike
      421 slekklqqll dkpvppavqv cedpeykakv eqceqelasl katcetqatd nrqlsdllae
      481 reaqaeelaa alerlredhv essrlleaiq sekvaasral sqnrelkrql eemqdvfvkl
      541 sndklelteq lqkeqhvtke lgerlgqqee elrelrdqla skegqvqgle qlssrehyqq
      601 sqladrvrhy qaqcqlteil qqelaqaqar tnalvtqnsd lrkalaekaq iavdvqekds
      661 skvhdlvasl sasvqqlele rdqlvhqlde qrtqreslqd qlfefkktee vllsheqeev
      721 tregyaklkr amqqledrfk atmeqianls dqkqrlehlv tqlqgetdti adyialyqvq
      781 rgimrkraae kddyisqlak dredlkakla elqclivrll eerqqqsgqs alplpgkvlt
      841 vssftdalqn gpsagdtgsa pctdsadevq dataqkimhl lteiesssav dgspvpdtfh
      901 pcpvcsgrll ti
//
LOCUS       XP_077504351             911 aa            linear   INV 25-NOV-2025
DEFINITION  golgin subfamily A member 2-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077504351
VERSION     XP_077504351.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648225.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..911
                     /product="golgin subfamily A member 2-like isoform X4"
                     /calculated_mol_wt=102847
     Region          <155..>316
                     /region_name="COG2433"
                     /note="Possible nuclease of RNase H fold, RuvC/YqgF family
                     [General function prediction only]"
                     /db_xref="CDD:441980"
     Region          373..822
                     /region_name="GOLGA2L5"
                     /note="Putative golgin subfamily A member 2-like protein
                     5; pfam15070"
                     /db_xref="CDD:464485"
     CDS             1..911
                     /gene="LOC144114473"
                     /coded_by="XM_077648225.1:1..2736"
                     /db_xref="GeneID:144114473"
ORIGIN      
        1 mrmsavsanm addvdrkhkv aaglkklkqf qqrsvkrttk rrssdtvspq ivqedqtsaq
       61 pldeqsssms qpskpkeala vtshscqdls qltddiqpsq rvlssaeslr qitmqlnglm
      121 edtsaalnge matladsssg alrsadallr rnqelatale alqqdrdqle fqlqelkgkl
      181 arqqrefdre reekeeqgrr eqgalndqlq vhiqtigilv aektelqsal sqsqqtakqk
      241 aveaeelqgr lkalrernsd lerklstasn qsqvqekstk esqrevdrlk tenyrlsksv
      301 eelrqelael sdklsrkske aeegqqelak vrqelslaql yaqqlggsqa seealgrmeq
      361 lhqeklhfer raaeykdame qiaaekqqma shyqsyvdsv sqqletyktk laemseikes
      421 lekklqqlld kpvppavqvc edpeykakve qceqelaslk atcetqatdn rqlsdllaer
      481 eaqaeelaaa lerlredhve ssrlleaiqs ekvaasrals qnrelkrqle emqdvfvkls
      541 ndklelteql qkeqhvtkel gerlgqqeee lrelrdqlas kegqvqgleq lssrehyqqs
      601 qladrvrhyq aqcqlteilq qelaqaqart nalvtqnsdl rkalaekaqi avdvqekdss
      661 kvhdlvasls asvqqleler dqlvhqldeq rtqreslqdq lfefkkteev llsheqeevt
      721 regyaklkra mqqledrfka tmeqianlsd qkqrlehlvt qlqgetdtia dyialyqvqr
      781 gimrkraaek ddyisqlakd redlkaklae lqclivrlle erqqqsgqsa lplpgkvltv
      841 ssftdalqng psagdtgsap ctdsadevqd ataqkimhll teiesssavd gspvpdtfhp
      901 cpvcsgrllt i
//
LOCUS       XP_077504352             843 aa            linear   INV 25-NOV-2025
DEFINITION  golgin subfamily A member 2-like isoform X5 [Amblyomma americanum].
ACCESSION   XP_077504352
VERSION     XP_077504352.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648226.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..843
                     /product="golgin subfamily A member 2-like isoform X5"
                     /calculated_mol_wt=95247
     Region          <87..>248
                     /region_name="COG2433"
                     /note="Possible nuclease of RNase H fold, RuvC/YqgF family
                     [General function prediction only]"
                     /db_xref="CDD:441980"
     Region          305..754
                     /region_name="GOLGA2L5"
                     /note="Putative golgin subfamily A member 2-like protein
                     5; pfam15070"
                     /db_xref="CDD:464485"
     CDS             1..843
                     /gene="LOC144114473"
                     /coded_by="XM_077648226.1:3581..6112"
                     /db_xref="GeneID:144114473"
ORIGIN      
        1 msqpskpkea lavtshscqd lsqltddiqp sqrvlssaes lrqitmqlng lmedtsaaln
       61 gematladss sgalrsadal lrrnqelata lealqqdrdq lefqlqelkg klarqqrefd
      121 rereekeeqg rreqgalndq lqvhiqtigi lvaektelqs alsqsqqtak qkaveaeelq
      181 grlkalrern sdlerklsta snqsqvqeks tkesqrevdr lktenyrlsk sveelrqela
      241 elsdklsrks keaeegqqel akvrqelsla qlyaqqlggs qaseealgrm eqlhqeklhf
      301 erraaeykda meqiaaekqq mashyqsyvd svsqqletyk tklaemseik eslekklqql
      361 ldkpvppavq vcedpeykak veqceqelas lkatcetqat dnrqlsdlla ereaqaeela
      421 aalerlredh vessrlleai qsekvaasra lsqnrelkrq leemqdvfvk lsndklelte
      481 qlqkeqhvtk elgerlgqqe eelrelrdql askegqvqgl eqlssrehyq qsqladrvrh
      541 yqaqcqltei lqqelaqaqa rtnalvtqns dlrkalaeka qiavdvqekd sskvhdlvas
      601 lsasvqqlel erdqlvhqld eqrtqreslq dqlfefkkte evllsheqee vtregyaklk
      661 ramqqledrf katmeqianl sdqkqrlehl vtqlqgetdt iadyialyqv qrgimrkraa
      721 ekddyisqla kdredlkakl aelqclivrl leerqqqsgq salplpgkvl tvssftdalq
      781 ngpsagdtgs apctdsadev qdataqkimh llteiesssa vdgspvpdtf hpcpvcsgrl
      841 lti
//
LOCUS       XP_077504353             482 aa            linear   INV 25-NOV-2025
DEFINITION  calcium-binding mitochondrial carrier protein SCaMC-2-like isoform
            X1 [Amblyomma americanum].
ACCESSION   XP_077504353
VERSION     XP_077504353.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648227.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..482
                     /product="calcium-binding mitochondrial carrier protein
                     SCaMC-2-like isoform X1"
                     /calculated_mol_wt=53833
     Region          30..160
                     /region_name="FRQ1"
                     /note="Ca2+-binding protein, EF-hand superfamily [Signal
                     transduction mechanisms]; COG5126"
                     /db_xref="CDD:444056"
     Region          208..458
                     /region_name="PTZ00169"
                     /note="ADP/ATP transporter on adenylate translocase;
                     Provisional"
                     /db_xref="CDD:240302"
     CDS             1..482
                     /gene="LOC144114474"
                     /coded_by="XM_077648227.1:370..1818"
                     /db_xref="GeneID:144114474"
ORIGIN      
        1 milqangapk ngqpqqhpph yypelpteve erleklfrkl danedgridi ndlatglrkl
       61 glphspgsve kfitasdtgk dgtvdfaefv hyiieheknl mvvfksldan sdgsldanei
      121 ktsfqslgih iehdeavrll krmdkdgslt isfeewrdyl lfhpsseihd iihhwrhatf
      181 vdigedslvp ddfteeeiht gmwwrhlvag gvagavsrtc tapldrlkvf lqvrgkefqs
      241 ihqclrhmlq eggvgslwrg nginvikiap esalkflaye kakrlikgds nrelgiferf
      301 fagslagsia qtsiypmevl ktrlalrktg qykgivdaay qiyrneglrs fykgylpnll
      361 giipyagidl aiyetlkkly lrqhdltddp gilvllgcgt vssscgqias yplalvrtrl
      421 qaqdgkhert smiglikgiv rtegfsglyr gitpnfmkva pavsisyvvy ehsrralgvs
      481 mt
//
LOCUS       XP_077504354             481 aa            linear   INV 25-NOV-2025
DEFINITION  calcium-binding mitochondrial carrier protein SCaMC-2-like isoform
            X2 [Amblyomma americanum].
ACCESSION   XP_077504354
VERSION     XP_077504354.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648228.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..481
                     /product="calcium-binding mitochondrial carrier protein
                     SCaMC-2-like isoform X2"
                     /calculated_mol_wt=53705
     Region          30..159
                     /region_name="FRQ1"
                     /note="Ca2+-binding protein, EF-hand superfamily [Signal
                     transduction mechanisms]; COG5126"
                     /db_xref="CDD:444056"
     Region          207..457
                     /region_name="PTZ00169"
                     /note="ADP/ATP transporter on adenylate translocase;
                     Provisional"
                     /db_xref="CDD:240302"
     CDS             1..481
                     /gene="LOC144114474"
                     /coded_by="XM_077648228.1:371..1816"
                     /db_xref="GeneID:144114474"
ORIGIN      
        1 milqangapk ngqpqqhpph yypelpteve erleklfrkl danedgridi ndlatglrkl
       61 glphspgsve fitasdtgkd gtvdfaefvh yiieheknlm vvfksldans dgsldaneik
      121 tsfqslgihi ehdeavrllk rmdkdgslti sfeewrdyll fhpsseihdi ihhwrhatfv
      181 digedslvpd dfteeeihtg mwwrhlvagg vagavsrtct apldrlkvfl qvrgkefqsi
      241 hqclrhmlqe ggvgslwrgn ginvikiape salkflayek akrlikgdsn relgiferff
      301 agslagsiaq tsiypmevlk trlalrktgq ykgivdaayq iyrneglrsf ykgylpnllg
      361 iipyagidla iyetlkklyl rqhdltddpg ilvllgcgtv ssscgqiasy plalvrtrlq
      421 aqdgkherts miglikgivr tegfsglyrg itpnfmkvap avsisyvvye hsrralgvsm
      481 t
//
LOCUS       XP_077504355             441 aa            linear   INV 25-NOV-2025
DEFINITION  calcium-binding mitochondrial carrier protein SCaMC-2-like isoform
            X3 [Amblyomma americanum].
ACCESSION   XP_077504355
VERSION     XP_077504355.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648229.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..441
                     /product="calcium-binding mitochondrial carrier protein
                     SCaMC-2-like isoform X3"
                     /calculated_mol_wt=49442
     Region          30..160
                     /region_name="FRQ1"
                     /note="Ca2+-binding protein, EF-hand superfamily [Signal
                     transduction mechanisms]; COG5126"
                     /db_xref="CDD:444056"
     Region          208..425
                     /region_name="PTZ00169"
                     /note="ADP/ATP transporter on adenylate translocase;
                     Provisional"
                     /db_xref="CDD:240302"
     CDS             1..441
                     /gene="LOC144114474"
                     /coded_by="XM_077648229.1:371..1696"
                     /db_xref="GeneID:144114474"
ORIGIN      
        1 milqangapk ngqpqqhpph yypelpteve erleklfrkl danedgridi ndlatglrkl
       61 glphspgsve kfitasdtgk dgtvdfaefv hyiieheknl mvvfksldan sdgsldanei
      121 ktsfqslgih iehdeavrll krmdkdgslt isfeewrdyl lfhpsseihd iihhwrhatf
      181 vdigedslvp ddfteeeiht gmwwrhlvag gvagavsrtc tapldrlkvf lqvrgkefqs
      241 ihqclrhmlq eggvgslwrg nginvikiap esalkflaye kakrlikgds nrelgiferf
      301 fagslagsia qtsiypmevl ktrlalrktg qykgivdaay qiyrneglrs fykgylpnll
      361 giipyagidl aiyetlkkly lrqhdltddp gilvllgcgt vssscgqias yplalvrtrl
      421 qaqagfncty kwtslkpgye e
//
LOCUS       XP_077504356             433 aa            linear   INV 25-NOV-2025
DEFINITION  calcium-binding mitochondrial carrier protein SCaMC-2-like isoform
            X4 [Amblyomma americanum].
ACCESSION   XP_077504356
VERSION     XP_077504356.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648230.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..433
                     /product="calcium-binding mitochondrial carrier protein
                     SCaMC-2-like isoform X4"
                     /calculated_mol_wt=48340
     Region          30..160
                     /region_name="FRQ1"
                     /note="Ca2+-binding protein, EF-hand superfamily [Signal
                     transduction mechanisms]; COG5126"
                     /db_xref="CDD:444056"
     Region          208..409
                     /region_name="PTZ00169"
                     /note="ADP/ATP transporter on adenylate translocase;
                     Provisional"
                     /db_xref="CDD:240302"
     Region          244..333
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     CDS             1..433
                     /gene="LOC144114474"
                     /coded_by="XM_077648230.1:372..1673"
                     /db_xref="GeneID:144114474"
ORIGIN      
        1 milqangapk ngqpqqhpph yypelpteve erleklfrkl danedgridi ndlatglrkl
       61 glphspgsve kfitasdtgk dgtvdfaefv hyiieheknl mvvfksldan sdgsldanei
      121 ktsfqslgih iehdeavrll krmdkdgslt isfeewrdyl lfhpsseihd iihhwrhatf
      181 vdigedslvp ddfteeeiht gmwwrhlvag gvagavsrtc tapldrlkvf lqakrlikgd
      241 snrelgifer ffagslagsi aqtsiypmev lktrlalrkt gqykgivdaa yqiyrneglr
      301 sfykgylpnl lgiipyagid laiyetlkkl ylrqhdltdd pgilvllgcg tvssscgqia
      361 syplalvrtr lqaqdgkher tsmiglikgi vrtegfsgly rgitpnfmkv apavsisyvv
      421 yehsrralgv smt
//
LOCUS       XP_077504357             382 aa            linear   INV 25-NOV-2025
DEFINITION  calcium-binding mitochondrial carrier protein SCaMC-2-A-like
            isoform X5 [Amblyomma americanum].
ACCESSION   XP_077504357
VERSION     XP_077504357.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648231.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..382
                     /product="calcium-binding mitochondrial carrier protein
                     SCaMC-2-A-like isoform X5"
                     /calculated_mol_wt=42602
     Region          3..58
                     /region_name="EFh"
                     /note="EF-hand, calcium binding motif; A diverse
                     superfamily of calcium sensors and calcium signal
                     modulators; most examples in this alignment model have 2
                     active canonical EF hands. Ca2+ binding induces a
                     conformational change in the EF-hand motif, leading to...;
                     cd00051"
                     /db_xref="CDD:238008"
     Site            order(8,10,12,19,44,46,48,55)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238008"
     Region          108..358
                     /region_name="PTZ00169"
                     /note="ADP/ATP transporter on adenylate translocase;
                     Provisional"
                     /db_xref="CDD:240302"
     CDS             1..382
                     /gene="LOC144114474"
                     /coded_by="XM_077648231.1:230..1378"
                     /db_xref="GeneID:144114474"
ORIGIN      
        1 mvvfksldan sdgsldanei ktsfqslgih iehdeavrll krmdkdgslt isfeewrdyl
       61 lfhpsseihd iihhwrhatf vdigedslvp ddfteeeiht gmwwrhlvag gvagavsrtc
      121 tapldrlkvf lqvrgkefqs ihqclrhmlq eggvgslwrg nginvikiap esalkflaye
      181 kakrlikgds nrelgiferf fagslagsia qtsiypmevl ktrlalrktg qykgivdaay
      241 qiyrneglrs fykgylpnll giipyagidl aiyetlkkly lrqhdltddp gilvllgcgt
      301 vssscgqias yplalvrtrl qaqdgkhert smiglikgiv rtegfsglyr gitpnfmkva
      361 pavsisyvvy ehsrralgvs mt
//
LOCUS       XP_077504358             908 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114476 [Amblyomma americanum].
ACCESSION   XP_077504358
VERSION     XP_077504358.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648232.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..908
                     /product="uncharacterized protein LOC144114476"
                     /calculated_mol_wt=97169
     Region          697..785
                     /region_name="CUB"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; cl00049"
                     /db_xref="CDD:412131"
     Region          807..836
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(819,822,826,832..833)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            829..833
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     CDS             1..908
                     /gene="LOC144114476"
                     /coded_by="XM_077648232.1:141..2867"
                     /db_xref="GeneID:144114476"
ORIGIN      
        1 mrtkvlsdag smifyltvvl avcgqglasr hklncldhea natqdiqhfa cttndsyslt
       61 inspnpgifi nmkvtmfpgd sfvlstvsgn dtrtlvsldg ehqaysvvyf ipaeivtisa
      121 nvtknskgdr tftgyfqegc nanlttttpv yhfppysngs giftcyitih asgskemdvy
      181 asldslilkg sshlnitgqp snfpavfkag avpefdfvas sltlaltldv siadqsgtvl
      241 idsvykncsg miemtsessk llllpanmte nflqrlscrw vvrgqdnnil gldvvsfsla
      301 gaedilvvtd ggtrnspvvl qasqgdahvt glvsrtssky iwvslavaey ntsdsfvarv
      361 tetaegghwk nqatgvrisd kskdsvylle varrmqvlls ihnmsltgsa svevvsdfyq
      421 ngpvlqkfsv gtelypvasl nnrlmlraqg fmpgnffvfs ftgvdpgchs ttlgtsglys
      481 lsgncnavct wainpsnssg ktqkftlmld hlnldnngnv sirslaqptk pllilnasys
      541 svkpvemssv sgayvvitrn kcaqengtva sgsvsgvlgs hfapklapgv syafqsplfp
      601 nqyplnstrs wtfdgtgigg fhltfmsmdi aqghalnlms gnqsvplngs tlpadilvee
      661 pkmyaqfaap ihpgyssgyg fnalltplds aqvvnketgq vetpsfpali nesktfvwsi
      721 yvpnfetkkm svailfnvsh lhksakgsgt ltvyngnsvr spvlgnltge nlltrtdtil
      781 vkfvtvageg salqlnftty rcnlsdtcnn skicihedwr cnginecgdn tdevgctynp
      841 tpspspttps ppgpqpspsk ggvstaafvv tvllalviga agalfvpvlv rryrayrysr
      901 fsnvavse
//
LOCUS       XP_077504360             845 aa            linear   INV 25-NOV-2025
DEFINITION  pre-mRNA-splicing factor SYF1 fand [Amblyomma americanum].
ACCESSION   XP_077504360
VERSION     XP_077504360.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648234.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..845
                     /product="pre-mRNA-splicing factor SYF1 fand"
                     /calculated_mol_wt=99136
     Region          <240..451
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Region          397..>595
                     /region_name="Spy"
                     /note="Predicted O-linked N-acetylglucosamine transferase,
                     SPINDLY family [Posttranslational modification, protein
                     turnover, chaperones]; COG3914"
                     /db_xref="CDD:443119"
     Site            order(477,480..481,484..485,487,511,514..515,518..519,
                     521..522,547,550,552,555..556,559)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          477..504
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          509..539
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          546..577
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     CDS             1..845
                     /gene="fand"
                     /coded_by="XM_077648234.1:154..2691"
                     /db_xref="GeneID:144114477"
ORIGIN      
        1 mkstkdilfe eddlpyeeei irnpfsvkhw mryidykkdq skyvinvice ralrelpgsy
       61 klwynylklr rqqvrdlcit dpeyedvnsa ferslvfmhk mpriwmdyck fltlqqkitr
      121 trrvfdralr alpitqhhri wplylefvnm heipetavrv yrrylklcpe naeefveylt
      181 rigrlddsav lladivnked fvskegkskh qlwnelcdli sknpdkvhsl kvdaiirggl
      241 rrytdqigql wnsladyyir aglferardi yeeaiqtvlt vrdftqvfda yaqfeesvlc
      301 akmeessktk psqesdlele lrlarfedlm drrplllnsv llrqnphnvh ewlkrvklfe
      361 gkpreiintf teavqtidpk latgklhvlw vsfakfyeen dqiedariif ekatqvpftk
      421 vedlahvwce waemelrhen hegalklmqk atampsrkva yhdqsepvqf rvykslkvws
      481 lyadleesfg tfkstkavyd riidlkiatp qiiinyglfl eennyfeeaf kayekgialf
      541 kwpnvfdiwn tyltkflkry ggtklerard lfeqclegcp akfakslyll yakleeehgl
      601 arhamgiydr gckavlpeeq femfniyilk aaeiygltht reiyeraiev lpdsqarlmc
      661 irfadlerkl geidraraiy ahcsqmcdpr ttadfwntwk efevhhgned tmremlrikr
      721 svqamyntqv nfmtaqmlsa tareaaaaag ndeapkdsmq qleaaaklla dearkdavrm
      781 pgkdilfvrg gttedeqsla ersrttnpde iniddeegsd ddqqieevav eqrsvpeevf
      841 gdlkk
//
LOCUS       XP_077504361             151 aa            linear   INV 25-NOV-2025
DEFINITION  small ribosomal subunit protein uS11 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504361
VERSION     XP_077504361.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648235.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..151
                     /product="small ribosomal subunit protein uS11 isoform X1"
                     /calculated_mol_wt=16045
     Region          1..138
                     /region_name="PTZ00129"
                     /note="40S ribosomal protein S14; Provisional"
                     /db_xref="CDD:185465"
     CDS             1..151
                     /gene="LOC144114478"
                     /coded_by="XM_077648235.1:141..596"
                     /db_xref="GeneID:144114478"
ORIGIN      
        1 maprkgkqke eqpivslgpq aignenvfgv ahiyasfndt fvhvtdlsgr etiarvtggm
       61 kvkadrdeas pyaamlaaqd vaekckqvgi talhiklrat ggtrtktpgp gaqsalrala
      121 rsdmkigrie dvtpipsdgt rrkggrrgrr l
//
LOCUS       XP_077504362             151 aa            linear   INV 25-NOV-2025
DEFINITION  small ribosomal subunit protein uS11 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504362
VERSION     XP_077504362.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648236.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..151
                     /product="small ribosomal subunit protein uS11 isoform X1"
                     /calculated_mol_wt=16045
     Region          1..138
                     /region_name="PTZ00129"
                     /note="40S ribosomal protein S14; Provisional"
                     /db_xref="CDD:185465"
     CDS             1..151
                     /gene="LOC144114478"
                     /coded_by="XM_077648236.1:112..567"
                     /db_xref="GeneID:144114478"
ORIGIN      
        1 maprkgkqke eqpivslgpq aignenvfgv ahiyasfndt fvhvtdlsgr etiarvtggm
       61 kvkadrdeas pyaamlaaqd vaekckqvgi talhiklrat ggtrtktpgp gaqsalrala
      121 rsdmkigrie dvtpipsdgt rrkggrrgrr l
//
LOCUS       XP_077504363             151 aa            linear   INV 25-NOV-2025
DEFINITION  small ribosomal subunit protein uS11 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504363
VERSION     XP_077504363.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648237.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..151
                     /product="small ribosomal subunit protein uS11 isoform X1"
                     /calculated_mol_wt=16045
     Region          1..138
                     /region_name="PTZ00129"
                     /note="40S ribosomal protein S14; Provisional"
                     /db_xref="CDD:185465"
     CDS             1..151
                     /gene="LOC144114478"
                     /coded_by="XM_077648237.1:105..560"
                     /db_xref="GeneID:144114478"
ORIGIN      
        1 maprkgkqke eqpivslgpq aignenvfgv ahiyasfndt fvhvtdlsgr etiarvtggm
       61 kvkadrdeas pyaamlaaqd vaekckqvgi talhiklrat ggtrtktpgp gaqsalrala
      121 rsdmkigrie dvtpipsdgt rrkggrrgrr l
//
LOCUS       XP_077504364             135 aa            linear   INV 25-NOV-2025
DEFINITION  small ribosomal subunit protein uS11 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504364
VERSION     XP_077504364.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648238.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..135
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..135
                     /product="small ribosomal subunit protein uS11 isoform X2"
                     /calculated_mol_wt=14485
     Region          1..>104
                     /region_name="Ribosomal_S11"
                     /note="Ribosomal protein S11; cl00332"
                     /db_xref="CDD:444843"
     CDS             1..135
                     /gene="LOC144114478"
                     /coded_by="XM_077648238.1:147..554"
                     /db_xref="GeneID:144114478"
ORIGIN      
        1 maprkgkqke eqpivslgpq aignenvfgv ahiyasfndt fvhvtdlsgr etiarvtggm
       61 kvkadrdeas pyaamlaaqd vaekckqvgi talhiklrat ggtrgcdths igrhtkerrp
      121 qrkasvnnga vniff
//
LOCUS       XP_077504365             314 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114479 [Amblyomma americanum].
ACCESSION   XP_077504365
VERSION     XP_077504365.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648239.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..314
                     /product="uncharacterized protein LOC144114479"
                     /calculated_mol_wt=32865
     CDS             1..314
                     /gene="LOC144114479"
                     /coded_by="XM_077648239.1:143..1087"
                     /db_xref="GeneID:144114479"
ORIGIN      
        1 mnccpllfal igcasysasl lttgsshqtt tdssgneltf vadriakgat tdqhtasstp
       61 sgqsaevlhr tsvpgrnwst aatgqqrgal ntasftsaar spapsssrpt pgqargtslq
      121 trgpsylsst taahaagata ssprhsehta tlkdptlcpe hasvqsmsss mtlsrssdvl
      181 nntnttstkv pnvpnessld nsstslmsne fgtvktdsls tnvlastkda ststslekpr
      241 srrskgaifa tvigaavllm gvivmgfwfy nvrwklnrer sllrnaeapd ttplpfsvvy
      301 yqndgtqglg lhrn
//
LOCUS       XP_077504366             303 aa            linear   INV 25-NOV-2025
DEFINITION  geranylgeranyl pyrophosphate synthase quemao [Amblyomma
            americanum].
ACCESSION   XP_077504366
VERSION     XP_077504366.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648240.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..303
                     /product="geranylgeranyl pyrophosphate synthase quemao"
                     /calculated_mol_wt=34779
     Region          18..259
                     /region_name="polyprenyl_synt"
                     /note="Polyprenyl synthetase; pfam00348"
                     /db_xref="CDD:459773"
     CDS             1..303
                     /gene="qm"
                     /coded_by="XM_077648240.1:121..1032"
                     /db_xref="GeneID:144114480"
ORIGIN      
        1 msrvmekhgp smtseemeka ilqpyvyivs ipgkqirtkl aqafnywlki spekcqavsd
       61 ivqmlhnssl liddiednsl lrrgvpvahh ifgvastins anyvyflslk kclqlgdlra
      121 ttvfseqcle lhrgqgmeiy wrdnyicpse edyismvkrk tgglfglavr lmqlfsenks
      181 dfteligilg lyfqirddya nlvhkeytdn ksfaedlteg kfsfpiihai qkhpedprim
      241 nivkkrttdl eikkyaldil erlgsfaytr eilvkldtda rreaeklggn pyivqvldel
      301 knw
//
LOCUS       XP_077504367             303 aa            linear   INV 25-NOV-2025
DEFINITION  geranylgeranyl pyrophosphate synthase quemao [Amblyomma
            americanum].
ACCESSION   XP_077504367
VERSION     XP_077504367.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648241.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..303
                     /product="geranylgeranyl pyrophosphate synthase quemao"
                     /calculated_mol_wt=34779
     Region          18..259
                     /region_name="polyprenyl_synt"
                     /note="Polyprenyl synthetase; pfam00348"
                     /db_xref="CDD:459773"
     CDS             1..303
                     /gene="qm"
                     /coded_by="XM_077648241.1:57..968"
                     /db_xref="GeneID:144114480"
ORIGIN      
        1 msrvmekhgp smtseemeka ilqpyvyivs ipgkqirtkl aqafnywlki spekcqavsd
       61 ivqmlhnssl liddiednsl lrrgvpvahh ifgvastins anyvyflslk kclqlgdlra
      121 ttvfseqcle lhrgqgmeiy wrdnyicpse edyismvkrk tgglfglavr lmqlfsenks
      181 dfteligilg lyfqirddya nlvhkeytdn ksfaedlteg kfsfpiihai qkhpedprim
      241 nivkkrttdl eikkyaldil erlgsfaytr eilvkldtda rreaeklggn pyivqvldel
      301 knw
//
LOCUS       XP_077504368             303 aa            linear   INV 25-NOV-2025
DEFINITION  geranylgeranyl pyrophosphate synthase quemao [Amblyomma
            americanum].
ACCESSION   XP_077504368
VERSION     XP_077504368.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648242.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..303
                     /product="geranylgeranyl pyrophosphate synthase quemao"
                     /calculated_mol_wt=34779
     Region          18..259
                     /region_name="polyprenyl_synt"
                     /note="Polyprenyl synthetase; pfam00348"
                     /db_xref="CDD:459773"
     CDS             1..303
                     /gene="qm"
                     /coded_by="XM_077648242.1:53..964"
                     /db_xref="GeneID:144114480"
ORIGIN      
        1 msrvmekhgp smtseemeka ilqpyvyivs ipgkqirtkl aqafnywlki spekcqavsd
       61 ivqmlhnssl liddiednsl lrrgvpvahh ifgvastins anyvyflslk kclqlgdlra
      121 ttvfseqcle lhrgqgmeiy wrdnyicpse edyismvkrk tgglfglavr lmqlfsenks
      181 dfteligilg lyfqirddya nlvhkeytdn ksfaedlteg kfsfpiihai qkhpedprim
      241 nivkkrttdl eikkyaldil erlgsfaytr eilvkldtda rreaeklggn pyivqvldel
      301 knw
//
LOCUS       XP_077504369             303 aa            linear   INV 25-NOV-2025
DEFINITION  geranylgeranyl pyrophosphate synthase quemao [Amblyomma
            americanum].
ACCESSION   XP_077504369
VERSION     XP_077504369.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648243.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..303
                     /product="geranylgeranyl pyrophosphate synthase quemao"
                     /calculated_mol_wt=34779
     Region          18..259
                     /region_name="polyprenyl_synt"
                     /note="Polyprenyl synthetase; pfam00348"
                     /db_xref="CDD:459773"
     CDS             1..303
                     /gene="qm"
                     /coded_by="XM_077648243.1:204..1115"
                     /db_xref="GeneID:144114480"
ORIGIN      
        1 msrvmekhgp smtseemeka ilqpyvyivs ipgkqirtkl aqafnywlki spekcqavsd
       61 ivqmlhnssl liddiednsl lrrgvpvahh ifgvastins anyvyflslk kclqlgdlra
      121 ttvfseqcle lhrgqgmeiy wrdnyicpse edyismvkrk tgglfglavr lmqlfsenks
      181 dfteligilg lyfqirddya nlvhkeytdn ksfaedlteg kfsfpiihai qkhpedprim
      241 nivkkrttdl eikkyaldil erlgsfaytr eilvkldtda rreaeklggn pyivqvldel
      301 knw
//
LOCUS       XP_077504370             358 aa            linear   INV 25-NOV-2025
DEFINITION  tetratricopeptide repeat domain 19 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504370
VERSION     XP_077504370.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648244.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..358
                     /product="tetratricopeptide repeat domain 19 isoform X1"
                     /calculated_mol_wt=40430
     Region          68..328
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Site            order(79..80,83..84,86,114,117..118,123..124,126..127,155,
                     162..163,166..167,170)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          79..103
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          108..144
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          154..182
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(224..225,227,259,262..263,266..267,269..270,298,
                     301..302,305..306,309)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          249..287
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          297..326
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     CDS             1..358
                     /gene="Ttc19"
                     /coded_by="XM_077648244.1:21..1097"
                     /db_xref="GeneID:144114482"
ORIGIN      
        1 masmassyrv lfrlvrrpfn yifrqssspt spisssslpq vtqsfcqrlp scksamlagl
       61 lsllglqere pedplihtik lgilnlqrqd fnkaesmlhi alkmaqered cqaetyifdi
      121 lanvafekgd ydkaeklfvd vmkrsiaagm prddnaivem slklaeiydh rkdtkkaeqg
      181 yrfcidaqek klekkdyler lselsdvekd tlvlwarste afgrhqleqg rileaqkcfe
      241 daltvsekvn gmehevtlsl lndvgtvasm lhdydralkl lgeaiakgrd iksnnlavfy
      301 cnlggilmee grsyaeaeda crealklatr tnnseaavrs recleelkkr apravfvr
//
LOCUS       XP_077504371             303 aa            linear   INV 25-NOV-2025
DEFINITION  tetratricopeptide repeat domain 19 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504371
VERSION     XP_077504371.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648245.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..303
                     /product="tetratricopeptide repeat domain 19 isoform X2"
                     /calculated_mol_wt=34258
     Region          13..273
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Site            order(169..170,172,204,207..208,211..212,214..215,243,
                     246..247,250..251,254)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          194..232
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          242..271
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     CDS             1..303
                     /gene="Ttc19"
                     /coded_by="XM_077648245.1:244..1155"
                     /db_xref="GeneID:144114482"
ORIGIN      
        1 mlagllsllg lqerepedpl ihtiklgiln lqrqdfnkae smlhialkma qeredcqaet
       61 yifdilanva fekgdydkae klfvdvmkrs iaagmprddn aivemslkla eiydhrkdtk
      121 kaeqgyrfci daqekklekk dylerlsels dvekdtlvlw arsteafgrh qleqgrilea
      181 qkcfedaltv sekvngmehe vtlsllndvg tvasmlhdyd ralkllgeai akgrdiksnn
      241 lavfycnlgg ilmeegrsya eaedacreal klatrtnnse aavrsrecle elkkraprav
      301 fvr
//
LOCUS       XP_077504374             254 aa            linear   INV 25-NOV-2025
DEFINITION  rhythmically expressed gene 2 protein-like [Amblyomma americanum].
ACCESSION   XP_077504374
VERSION     XP_077504374.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648248.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..254
                     /product="rhythmically expressed gene 2 protein-like"
                     /calculated_mol_wt=28197
     Region          6..212
                     /region_name="DREG-2"
                     /note="REG-2-like, HAD superfamily (subfamily IA)
                     hydrolase; TIGR02252"
                     /db_xref="CDD:274056"
     CDS             1..254
                     /gene="LOC144114483"
                     /coded_by="XM_077648248.1:258..1022"
                     /db_xref="GeneID:144114483"
ORIGIN      
        1 mvsrlrlitf datntllryk esvgqiysgv aqlygvpadp hhvnqkfrie fkrmvaqhpn
       61 ygcdsgmtsq qwwaelvsrt lsgsgaises lmtsiskhly esyrtpecwa pnvgtvetlq
      121 rlknsgrklg visntderld siltglrlrh yfdfviasav vkvekpskdi fslalicass
      181 dehlkpdral hvgdnieldy laaksagwna lllvndeshr hralskglvd padmiqrlad
      241 ivkvvdaqes qcat
//
LOCUS       XP_077504375             352 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114484 [Amblyomma americanum].
ACCESSION   XP_077504375
VERSION     XP_077504375.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648249.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..352
                     /product="uncharacterized protein LOC144114484"
                     /calculated_mol_wt=38657
     CDS             1..352
                     /gene="LOC144114484"
                     /coded_by="XM_077648249.1:110..1168"
                     /db_xref="GeneID:144114484"
ORIGIN      
        1 milqrnvfrl ellarvppap kapsdedclh makkaarsid egplflllvd gakvksrseq
       61 tssaqvrnhg tlltclfdpe eprvvttifl ldadddsgdr kkevavwraa teeearalcn
      121 fkdplpknes lssspsdtls slnslsstgs sdslkassgr qvlvaasrsa srdrradsrq
      181 reapkvlppp pgeqersgrk sdssrkessr klphlwtaaa nslkrapqil vrpagnqalq
      241 pgpsswmrws pprvpvmcfq pfsrplvnqd grgfrpnips qlphhakgss rntaallhsp
      301 qrsmlrsgsl ssqesggsdn eghhqatefd hhsrhrskkv vtfnymtkvq fm
//
LOCUS       XP_077504376             311 aa            linear   INV 25-NOV-2025
DEFINITION  tRNA methyltransferase 10 homolog B-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504376
VERSION     XP_077504376.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648250.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..311
                     /product="tRNA methyltransferase 10 homolog B-like isoform
                     X1"
                     /calculated_mol_wt=34578
     Region          109..290
                     /region_name="SPOUT_MTase"
                     /note="SPOUT superfamily of SAM-dependent RNA
                     methyltransferases; cl38912"
                     /db_xref="CDD:476814"
     Site            order(195..197,216..219,241..243,260,262,265)
                     /site_type="other"
                     /note="SAM binding site [chemical binding]"
                     /db_xref="CDD:349952"
     CDS             1..311
                     /gene="LOC144114485"
                     /coded_by="XM_077648250.1:133..1068"
                     /db_xref="GeneID:144114485"
ORIGIN      
        1 mdagssalee sslpasvnep qnrnvpvavp ptlsknvark laryervvta rrakrrekkl
       61 arktriaeak kleqsdaksv pgpeqrsdgq lreqelnhns saghevpapc vcidmghtdy
      121 mtdkelnkls sqlrrlygsn rhscnplrly fthfptscrl yklcvekhqg ferysvemta
      181 eshiklfpke sliylspdap dalpatplgp divyiigglv detvhknisl staraegirc
      241 aqlpidefme rcggtgckil tvnqvfdill svqetkgdwa dslakvipkr tglrkrqtvm
      301 dtncktehdg l
//
LOCUS       XP_077504377             311 aa            linear   INV 25-NOV-2025
DEFINITION  tRNA methyltransferase 10 homolog B-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504377
VERSION     XP_077504377.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648251.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..311
                     /product="tRNA methyltransferase 10 homolog B-like isoform
                     X1"
                     /calculated_mol_wt=34578
     Region          109..290
                     /region_name="SPOUT_MTase"
                     /note="SPOUT superfamily of SAM-dependent RNA
                     methyltransferases; cl38912"
                     /db_xref="CDD:476814"
     Site            order(195..197,216..219,241..243,260,262,265)
                     /site_type="other"
                     /note="SAM binding site [chemical binding]"
                     /db_xref="CDD:349952"
     CDS             1..311
                     /gene="LOC144114485"
                     /coded_by="XM_077648251.1:85..1020"
                     /db_xref="GeneID:144114485"
ORIGIN      
        1 mdagssalee sslpasvnep qnrnvpvavp ptlsknvark laryervvta rrakrrekkl
       61 arktriaeak kleqsdaksv pgpeqrsdgq lreqelnhns saghevpapc vcidmghtdy
      121 mtdkelnkls sqlrrlygsn rhscnplrly fthfptscrl yklcvekhqg ferysvemta
      181 eshiklfpke sliylspdap dalpatplgp divyiigglv detvhknisl staraegirc
      241 aqlpidefme rcggtgckil tvnqvfdill svqetkgdwa dslakvipkr tglrkrqtvm
      301 dtncktehdg l
//
LOCUS       XP_077504378             311 aa            linear   INV 25-NOV-2025
DEFINITION  tRNA methyltransferase 10 homolog B-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504378
VERSION     XP_077504378.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648252.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..311
                     /product="tRNA methyltransferase 10 homolog B-like isoform
                     X1"
                     /calculated_mol_wt=34578
     Region          109..290
                     /region_name="SPOUT_MTase"
                     /note="SPOUT superfamily of SAM-dependent RNA
                     methyltransferases; cl38912"
                     /db_xref="CDD:476814"
     Site            order(195..197,216..219,241..243,260,262,265)
                     /site_type="other"
                     /note="SAM binding site [chemical binding]"
                     /db_xref="CDD:349952"
     CDS             1..311
                     /gene="LOC144114485"
                     /coded_by="XM_077648252.1:82..1017"
                     /db_xref="GeneID:144114485"
ORIGIN      
        1 mdagssalee sslpasvnep qnrnvpvavp ptlsknvark laryervvta rrakrrekkl
       61 arktriaeak kleqsdaksv pgpeqrsdgq lreqelnhns saghevpapc vcidmghtdy
      121 mtdkelnkls sqlrrlygsn rhscnplrly fthfptscrl yklcvekhqg ferysvemta
      181 eshiklfpke sliylspdap dalpatplgp divyiigglv detvhknisl staraegirc
      241 aqlpidefme rcggtgckil tvnqvfdill svqetkgdwa dslakvipkr tglrkrqtvm
      301 dtncktehdg l
//
LOCUS       XP_077504379             311 aa            linear   INV 25-NOV-2025
DEFINITION  tRNA methyltransferase 10 homolog B-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504379
VERSION     XP_077504379.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648253.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..311
                     /product="tRNA methyltransferase 10 homolog B-like isoform
                     X1"
                     /calculated_mol_wt=34578
     Region          109..290
                     /region_name="SPOUT_MTase"
                     /note="SPOUT superfamily of SAM-dependent RNA
                     methyltransferases; cl38912"
                     /db_xref="CDD:476814"
     Site            order(195..197,216..219,241..243,260,262,265)
                     /site_type="other"
                     /note="SAM binding site [chemical binding]"
                     /db_xref="CDD:349952"
     CDS             1..311
                     /gene="LOC144114485"
                     /coded_by="XM_077648253.1:96..1031"
                     /db_xref="GeneID:144114485"
ORIGIN      
        1 mdagssalee sslpasvnep qnrnvpvavp ptlsknvark laryervvta rrakrrekkl
       61 arktriaeak kleqsdaksv pgpeqrsdgq lreqelnhns saghevpapc vcidmghtdy
      121 mtdkelnkls sqlrrlygsn rhscnplrly fthfptscrl yklcvekhqg ferysvemta
      181 eshiklfpke sliylspdap dalpatplgp divyiigglv detvhknisl staraegirc
      241 aqlpidefme rcggtgckil tvnqvfdill svqetkgdwa dslakvipkr tglrkrqtvm
      301 dtncktehdg l
//
LOCUS       XP_077504380             308 aa            linear   INV 25-NOV-2025
DEFINITION  tRNA methyltransferase 10 homolog B-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504380
VERSION     XP_077504380.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648254.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..308
                     /product="tRNA methyltransferase 10 homolog B-like isoform
                     X2"
                     /calculated_mol_wt=34286
     Region          109..264
                     /region_name="SPOUT_MTase"
                     /note="SPOUT superfamily of SAM-dependent RNA
                     methyltransferases; cl38912"
                     /db_xref="CDD:476814"
     Site            order(195..197,216..219,241..243,260,262)
                     /site_type="other"
                     /note="SAM binding site [chemical binding]"
                     /db_xref="CDD:349952"
     CDS             1..308
                     /gene="LOC144114485"
                     /coded_by="XM_077648254.1:133..1059"
                     /db_xref="GeneID:144114485"
ORIGIN      
        1 mdagssalee sslpasvnep qnrnvpvavp ptlsknvark laryervvta rrakrrekkl
       61 arktriaeak kleqsdaksv pgpeqrsdgq lreqelnhns saghevpapc vcidmghtdy
      121 mtdkelnkls sqlrrlygsn rhscnplrly fthfptscrl yklcvekhqg ferysvemta
      181 eshiklfpke sliylspdap dalpatplgp divyiigglv detvhknisl staraegirc
      241 aqlpidefme rcggtgckil tvnqarrchh hvtsgggaps psfishnrrh rdrryqeskq
      301 rpslstat
//
LOCUS       XP_077504381             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114486 [Amblyomma americanum].
ACCESSION   XP_077504381
VERSION     XP_077504381.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648255.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144114486"
                     /calculated_mol_wt=24744
     CDS             1..217
                     /gene="LOC144114486"
                     /coded_by="XM_077648255.1:127..780"
                     /db_xref="GeneID:144114486"
ORIGIN      
        1 meskidslla mssklesles svsllsdkfd efqtrllaqe kstkdvtkrv erlekaewac
       61 eiaqlnkdvd slewrsrrln ieihglpete nedllkkvne igtkleldev cssditalhr
      121 lpakpgktrg vivrfakqev rdawlakrqa lrddntgkym cenmtrytrt lltvakewak
      181 esgyafvwhv ngkvlvrkqa garavvirdk ddlanlr
//
LOCUS       XP_077504382             135 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114487 [Amblyomma americanum].
ACCESSION   XP_077504382
VERSION     XP_077504382.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648256.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..135
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..135
                     /product="uncharacterized protein LOC144114487"
                     /calculated_mol_wt=15254
     CDS             1..135
                     /gene="LOC144114487"
                     /coded_by="XM_077648256.1:45..452"
                     /db_xref="GeneID:144114487"
ORIGIN      
        1 maekpcphgg afhrghpgkt nmkvllmlcv lvaiaecaka kttkipssta psfctqsqkt
       61 klntfrcvlr rssrkfqrkv ktfrkahymn erqfinalcs pnmnllpplr rtfttkdmkn
      121 vlrnavkcgr hfyng
//
LOCUS       XP_077504383             121 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114488 [Amblyomma americanum].
ACCESSION   XP_077504383
VERSION     XP_077504383.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648257.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..121
                     /product="uncharacterized protein LOC144114488"
                     /calculated_mol_wt=13904
     CDS             1..121
                     /gene="LOC144114488"
                     /coded_by="XM_077648257.1:175..540"
                     /db_xref="GeneID:144114488"
ORIGIN      
        1 mrvlalflla lvicgttaqq pakkkralfc vlpdqyrvla lecmwthsyr evqamiksil
       61 vkrqwgakhl ahglcnpyei fklkkffkgk ieqkllqeai araelcipyi lknyinreaa
      121 q
//
LOCUS       XP_077504384             377 aa            linear   INV 25-NOV-2025
DEFINITION  putative G-protein coupled receptor B0563.6 [Amblyomma americanum].
ACCESSION   XP_077504384
VERSION     XP_077504384.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648258.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..377
                     /product="putative G-protein coupled receptor B0563.6"
                     /calculated_mol_wt=41970
     Region          54..347
                     /region_name="7tmA_FMRFamide_R-like"
                     /note="FMRFamide (Phe-Met-Arg-Phe) receptors and related
                     proteins, member of the class A family of
                     seven-transmembrane G protein-coupled receptors; cd14978"
                     /db_xref="CDD:410630"
     Region          55..81
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410630"
     Region          87..112
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410630"
     Site            order(109,112,127..132,134..135,138,183,185..189,222,
                     225..227,229..231,233..234,293,296..297,299..300,303,
                     314..315,317..319,322,325..326)
                     /site_type="other"
                     /note="putative ligand binding pocket [chemical binding]"
                     /db_xref="CDD:410630"
     Region          127..157
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410630"
     Region          169..189
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410630"
     Region          222..247
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410630"
     Region          274..304
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410630"
     Region          315..340
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410630"
     CDS             1..377
                     /gene="LOC144114489"
                     /coded_by="XM_077648258.1:966..2099"
                     /db_xref="GeneID:144114489"
ORIGIN      
        1 mtkamvvcpn ynappaaand sgvassavpw peaqagdgrs vaqlkedial lkfiaygvvs
       61 fvvialgivg nlinlvvltr pnlkgvtfvy ltwlatsdll tlvvavfsml rlhgiqprsy
      121 paafyyahve mplvnalmas svfivlavtm drywsvcrpt ryrefhnarr aklailsafv
      181 gaallyipva fqkmpvpfwd ehanmtqymp cdnvlvsqht afkvyllvke vlvrvgpvfv
      241 vaalnttiiv afrklmrkrq llmnsstrds gkfleeqrlv illaaivimf cvcmtpaail
      301 tvlisddkel nygfqlfraf andmemanfa mnfyvyclcs seiretffrl frfsrppste
      361 vsyvisskta vdgstrl
//
LOCUS       XP_077504385             942 aa            linear   INV 25-NOV-2025
DEFINITION  ras GTPase-activating protein raskol isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504385
VERSION     XP_077504385.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648259.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..942
                     /product="ras GTPase-activating protein raskol isoform X1"
                     /calculated_mol_wt=104110
     Region          39..228
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          170..314
                     /region_name="C2_SynGAP_like"
                     /note="C2 domain present in Ras GTPase activating protein
                     (GAP) family; cd04013"
                     /db_xref="CDD:175980"
     Region          305..627
                     /region_name="RasGAP_DAB2IP"
                     /note="Ras-GTPase Activating Domain of DAB2IP and similar
                     proteins; cd05136"
                     /db_xref="CDD:213338"
     Site            order(344,380,382,384..385,387,390,394,489,497..498,
                     501..502,505,526,529..530,533,537,539,544..545)
                     /site_type="other"
                     /note="putative RAS interface [polypeptide binding]"
                     /db_xref="CDD:213338"
     Region          <838..914
                     /region_name="DUF3498"
                     /note="Domain of unknown function (DUF3498); pfam12004"
                     /db_xref="CDD:463427"
     CDS             1..942
                     /gene="raskol"
                     /coded_by="XM_077648259.1:142..2970"
                     /db_xref="GeneID:144114490"
ORIGIN      
        1 maldclaspe qeaaqergsd evrqggsats tpvlggrvpd atatpsrivs ffskrslkqn
       61 plkrtksvsk lerarraeap qprlrgsrsh esllqspggp wglegarlgp lhpslrnhpt
      121 qgqawgpapr gagehwfela prdgpacvya crsrqqclhw larlrqaaqp qrdavrrten
      181 glrlwvleak glapkkryyc elrldgtlya rtsaklkaql cfwgehfefs hlpavgsltv
      241 tvfreadrrk rrdkstalgm vtvplvpara hhvtekwypl vpelkgpptp gakestppsv
      301 rlkwryqsle vqplasyqpf lqylkrdygp lcrllepvlg vrlkeeiatg lvsimhreem
      361 aqefltqlvm adlqdigdqh ltfrgnslat kateaylkll gdgylqetlg pfirdvlasn
      421 ldcevdpakv sggsgalqrq qsllrqrvqq awqritqsah sfpkdlrmlf hgyrqrlggn
      481 theeelcdnl isgsiflrcl cpailspslf glapeypder aarsltliak tiqtlanftr
      541 fggkesfmef mndfvekews tmktflkvis spvsaetnss gseagievpc vvdlglelaq
      601 lqmmlreclp qlegkegeaa lraaldgrpr srgqvlscle anpapapqpr sstlprsala
      661 psanmlrevp ralpdllttm pssqgptavg rvlppqpggc pplafsnpvy rwgglqepqp
      721 esltggesgs sghssaaese asgtsspssp hlwednflga agelagrsre gderlacrsl
      781 relganlhda stnmqyerev aqlkrlmgql qrklslaetq lqqqqqqqhp ltaslgaest
      841 eeqqqrrqek dlqmksiitr litvedelrr eqremqgmir akqqlidaqe rkiqtldaan
      901 qrllgalaql rqhyqdqvql dprllangas spppptyrss sc
//
LOCUS       XP_077504386             929 aa            linear   INV 25-NOV-2025
DEFINITION  ras GTPase-activating protein raskol isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504386
VERSION     XP_077504386.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648260.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..929
                     /product="ras GTPase-activating protein raskol isoform X2"
                     /calculated_mol_wt=102756
     Region          39..228
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          170..314
                     /region_name="C2_SynGAP_like"
                     /note="C2 domain present in Ras GTPase activating protein
                     (GAP) family; cd04013"
                     /db_xref="CDD:175980"
     Region          305..627
                     /region_name="RasGAP_DAB2IP"
                     /note="Ras-GTPase Activating Domain of DAB2IP and similar
                     proteins; cd05136"
                     /db_xref="CDD:213338"
     Site            order(344,380,382,384..385,387,390,394,489,497..498,
                     501..502,505,526,529..530,533,537,539,544..545)
                     /site_type="other"
                     /note="putative RAS interface [polypeptide binding]"
                     /db_xref="CDD:213338"
     Region          <774..901
                     /region_name="DUF3498"
                     /note="Domain of unknown function (DUF3498); pfam12004"
                     /db_xref="CDD:463427"
     CDS             1..929
                     /gene="raskol"
                     /coded_by="XM_077648260.1:142..2931"
                     /db_xref="GeneID:144114490"
ORIGIN      
        1 maldclaspe qeaaqergsd evrqggsats tpvlggrvpd atatpsrivs ffskrslkqn
       61 plkrtksvsk lerarraeap qprlrgsrsh esllqspggp wglegarlgp lhpslrnhpt
      121 qgqawgpapr gagehwfela prdgpacvya crsrqqclhw larlrqaaqp qrdavrrten
      181 glrlwvleak glapkkryyc elrldgtlya rtsaklkaql cfwgehfefs hlpavgsltv
      241 tvfreadrrk rrdkstalgm vtvplvpara hhvtekwypl vpelkgpptp gakestppsv
      301 rlkwryqsle vqplasyqpf lqylkrdygp lcrllepvlg vrlkeeiatg lvsimhreem
      361 aqefltqlvm adlqdigdqh ltfrgnslat kateaylkll gdgylqetlg pfirdvlasn
      421 ldcevdpakv sggsgalqrq qsllrqrvqq awqritqsah sfpkdlrmlf hgyrqrlggn
      481 theeelcdnl isgsiflrcl cpailspslf glapeypder aarsltliak tiqtlanftr
      541 fggkesfmef mndfvekews tmktflkvis spvsaetnss gseagievpc vvdlglelaq
      601 lqmmlreclp qlegkegeaa lraaldgrpr srgqvlscle anpapapqpr sstlprsala
      661 psanmlrevp ralpdllttm pssqgptavg rvlppqpggc pplafsnpvy rwgglqepqp
      721 esltggesgs sghssaaese asgtsspssp hlwednflga agelagrsre gderlacrsl
      781 reyerevaql krlmgqlqrk lslaetqlqq qqqqqhplta slgaesteeq qqrrqekdlq
      841 mksiitrlit vedelrreqr emqgmirakq qlidaqerki qtldaanqrl lgalaqlrqh
      901 yqdqvqldpr llangasspp pptyrsssc
//
LOCUS       XP_077504387            1062 aa            linear   INV 25-NOV-2025
DEFINITION  ras GTPase-activating protein raskol isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504387
VERSION     XP_077504387.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648261.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1062
                     /product="ras GTPase-activating protein raskol isoform X3"
                     /calculated_mol_wt=117970
     Region          159..348
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          290..434
                     /region_name="C2_SynGAP_like"
                     /note="C2 domain present in Ras GTPase activating protein
                     (GAP) family; cd04013"
                     /db_xref="CDD:175980"
     Region          425..747
                     /region_name="RasGAP_DAB2IP"
                     /note="Ras-GTPase Activating Domain of DAB2IP and similar
                     proteins; cd05136"
                     /db_xref="CDD:213338"
     Site            order(464,500,502,504..505,507,510,514,609,617..618,
                     621..622,625,646,649..650,653,657,659,664..665)
                     /site_type="other"
                     /note="putative RAS interface [polypeptide binding]"
                     /db_xref="CDD:213338"
     Region          <958..1034
                     /region_name="DUF3498"
                     /note="Domain of unknown function (DUF3498); pfam12004"
                     /db_xref="CDD:463427"
     CDS             1..1062
                     /gene="raskol"
                     /coded_by="XM_077648261.1:265..3453"
                     /db_xref="GeneID:144114490"
ORIGIN      
        1 mqdsslssqd lqgrlegwly vceleagpep rapalkrarh qgrcwqqrfc vllpdkktlt
       61 hfpsediarr clhgrpwgad wdrvlpeede gplsetgslr ddrllgccsp pelyatvahl
      121 kgfrhimrcq rkaaqergsd evrqggsats tpvlggrvpd atatpsrivs ffskrslkqn
      181 plkrtksvsk lerarraeap qprlrgsrsh esllqspggp wglegarlgp lhpslrnhpt
      241 qgqawgpapr gagehwfela prdgpacvya crsrqqclhw larlrqaaqp qrdavrrten
      301 glrlwvleak glapkkryyc elrldgtlya rtsaklkaql cfwgehfefs hlpavgsltv
      361 tvfreadrrk rrdkstalgm vtvplvpara hhvtekwypl vpelkgpptp gakestppsv
      421 rlkwryqsle vqplasyqpf lqylkrdygp lcrllepvlg vrlkeeiatg lvsimhreem
      481 aqefltqlvm adlqdigdqh ltfrgnslat kateaylkll gdgylqetlg pfirdvlasn
      541 ldcevdpakv sggsgalqrq qsllrqrvqq awqritqsah sfpkdlrmlf hgyrqrlggn
      601 theeelcdnl isgsiflrcl cpailspslf glapeypder aarsltliak tiqtlanftr
      661 fggkesfmef mndfvekews tmktflkvis spvsaetnss gseagievpc vvdlglelaq
      721 lqmmlreclp qlegkegeaa lraaldgrpr srgqvlscle anpapapqpr sstlprsala
      781 psanmlrevp ralpdllttm pssqgptavg rvlppqpggc pplafsnpvy rwgglqepqp
      841 esltggesgs sghssaaese asgtsspssp hlwednflga agelagrsre gderlacrsl
      901 relganlhda stnmqyerev aqlkrlmgql qrklslaetq lqqqqqqqhp ltaslgaest
      961 eeqqqrrqek dlqmksiitr litvedelrr eqremqgmir akqqlidaqe rkiqtldaan
     1021 qrllgalaql rqhyqdqvql dprllangas spppptyrss sc
//
LOCUS       XP_077504388            1042 aa            linear   INV 25-NOV-2025
DEFINITION  ras GTPase-activating protein raskol isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077504388
VERSION     XP_077504388.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648262.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1042
                     /product="ras GTPase-activating protein raskol isoform X4"
                     /calculated_mol_wt=115559
     Region          139..328
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          270..414
                     /region_name="C2_SynGAP_like"
                     /note="C2 domain present in Ras GTPase activating protein
                     (GAP) family; cd04013"
                     /db_xref="CDD:175980"
     Region          405..727
                     /region_name="RasGAP_DAB2IP"
                     /note="Ras-GTPase Activating Domain of DAB2IP and similar
                     proteins; cd05136"
                     /db_xref="CDD:213338"
     Site            order(444,480,482,484..485,487,490,494,589,597..598,
                     601..602,605,626,629..630,633,637,639,644..645)
                     /site_type="other"
                     /note="putative RAS interface [polypeptide binding]"
                     /db_xref="CDD:213338"
     Region          <938..1014
                     /region_name="DUF3498"
                     /note="Domain of unknown function (DUF3498); pfam12004"
                     /db_xref="CDD:463427"
     CDS             1..1042
                     /gene="raskol"
                     /coded_by="XM_077648262.1:265..3393"
                     /db_xref="GeneID:144114490"
ORIGIN      
        1 mqdsslssqd lqgrlegwly vceleagpep rapalkrarh qgrcwqqrfc vllpdkktlt
       61 hfpsediarr clhgrpwgad wdrvlpeede gplsetgslr ddrllgccsp peaaqergsd
      121 evrqggsats tpvlggrvpd atatpsrivs ffskrslkqn plkrtksvsk lerarraeap
      181 qprlrgsrsh esllqspggp wglegarlgp lhpslrnhpt qgqawgpapr gagehwfela
      241 prdgpacvya crsrqqclhw larlrqaaqp qrdavrrten glrlwvleak glapkkryyc
      301 elrldgtlya rtsaklkaql cfwgehfefs hlpavgsltv tvfreadrrk rrdkstalgm
      361 vtvplvpara hhvtekwypl vpelkgpptp gakestppsv rlkwryqsle vqplasyqpf
      421 lqylkrdygp lcrllepvlg vrlkeeiatg lvsimhreem aqefltqlvm adlqdigdqh
      481 ltfrgnslat kateaylkll gdgylqetlg pfirdvlasn ldcevdpakv sggsgalqrq
      541 qsllrqrvqq awqritqsah sfpkdlrmlf hgyrqrlggn theeelcdnl isgsiflrcl
      601 cpailspslf glapeypder aarsltliak tiqtlanftr fggkesfmef mndfvekews
      661 tmktflkvis spvsaetnss gseagievpc vvdlglelaq lqmmlreclp qlegkegeaa
      721 lraaldgrpr srgqvlscle anpapapqpr sstlprsala psanmlrevp ralpdllttm
      781 pssqgptavg rvlppqpggc pplafsnpvy rwgglqepqp esltggesgs sghssaaese
      841 asgtsspssp hlwednflga agelagrsre gderlacrsl relganlhda stnmqyerev
      901 aqlkrlmgql qrklslaetq lqqqqqqqhp ltaslgaest eeqqqrrqek dlqmksiitr
      961 litvedelrr eqremqgmir akqqlidaqe rkiqtldaan qrllgalaql rqhyqdqvql
     1021 dprllangas spppptyrss sc
//
LOCUS       XP_077504389            1029 aa            linear   INV 25-NOV-2025
DEFINITION  ras GTPase-activating protein raskol isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077504389
VERSION     XP_077504389.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648263.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1029
                     /product="ras GTPase-activating protein raskol isoform X5"
                     /calculated_mol_wt=114205
     Region          139..328
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          270..414
                     /region_name="C2_SynGAP_like"
                     /note="C2 domain present in Ras GTPase activating protein
                     (GAP) family; cd04013"
                     /db_xref="CDD:175980"
     Region          405..727
                     /region_name="RasGAP_DAB2IP"
                     /note="Ras-GTPase Activating Domain of DAB2IP and similar
                     proteins; cd05136"
                     /db_xref="CDD:213338"
     Site            order(444,480,482,484..485,487,490,494,589,597..598,
                     601..602,605,626,629..630,633,637,639,644..645)
                     /site_type="other"
                     /note="putative RAS interface [polypeptide binding]"
                     /db_xref="CDD:213338"
     Region          <874..1001
                     /region_name="DUF3498"
                     /note="Domain of unknown function (DUF3498); pfam12004"
                     /db_xref="CDD:463427"
     CDS             1..1029
                     /gene="raskol"
                     /coded_by="XM_077648263.1:265..3354"
                     /db_xref="GeneID:144114490"
ORIGIN      
        1 mqdsslssqd lqgrlegwly vceleagpep rapalkrarh qgrcwqqrfc vllpdkktlt
       61 hfpsediarr clhgrpwgad wdrvlpeede gplsetgslr ddrllgccsp peaaqergsd
      121 evrqggsats tpvlggrvpd atatpsrivs ffskrslkqn plkrtksvsk lerarraeap
      181 qprlrgsrsh esllqspggp wglegarlgp lhpslrnhpt qgqawgpapr gagehwfela
      241 prdgpacvya crsrqqclhw larlrqaaqp qrdavrrten glrlwvleak glapkkryyc
      301 elrldgtlya rtsaklkaql cfwgehfefs hlpavgsltv tvfreadrrk rrdkstalgm
      361 vtvplvpara hhvtekwypl vpelkgpptp gakestppsv rlkwryqsle vqplasyqpf
      421 lqylkrdygp lcrllepvlg vrlkeeiatg lvsimhreem aqefltqlvm adlqdigdqh
      481 ltfrgnslat kateaylkll gdgylqetlg pfirdvlasn ldcevdpakv sggsgalqrq
      541 qsllrqrvqq awqritqsah sfpkdlrmlf hgyrqrlggn theeelcdnl isgsiflrcl
      601 cpailspslf glapeypder aarsltliak tiqtlanftr fggkesfmef mndfvekews
      661 tmktflkvis spvsaetnss gseagievpc vvdlglelaq lqmmlreclp qlegkegeaa
      721 lraaldgrpr srgqvlscle anpapapqpr sstlprsala psanmlrevp ralpdllttm
      781 pssqgptavg rvlppqpggc pplafsnpvy rwgglqepqp esltggesgs sghssaaese
      841 asgtsspssp hlwednflga agelagrsre gderlacrsl reyerevaql krlmgqlqrk
      901 lslaetqlqq qqqqqhplta slgaesteeq qqrrqekdlq mksiitrlit vedelrreqr
      961 emqgmirakq qlidaqerki qtldaanqrl lgalaqlrqh yqdqvqldpr llangasspp
     1021 pptyrsssc
//
LOCUS       XP_077504390             932 aa            linear   INV 25-NOV-2025
DEFINITION  ras GTPase-activating protein raskol isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077504390
VERSION     XP_077504390.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648264.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..932
                     /product="ras GTPase-activating protein raskol isoform X6"
                     /calculated_mol_wt=103081
     Region          29..218
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          160..304
                     /region_name="C2_SynGAP_like"
                     /note="C2 domain present in Ras GTPase activating protein
                     (GAP) family; cd04013"
                     /db_xref="CDD:175980"
     Region          295..617
                     /region_name="RasGAP_DAB2IP"
                     /note="Ras-GTPase Activating Domain of DAB2IP and similar
                     proteins; cd05136"
                     /db_xref="CDD:213338"
     Site            order(334,370,372,374..375,377,380,384,479,487..488,
                     491..492,495,516,519..520,523,527,529,534..535)
                     /site_type="other"
                     /note="putative RAS interface [polypeptide binding]"
                     /db_xref="CDD:213338"
     Region          <828..904
                     /region_name="DUF3498"
                     /note="Domain of unknown function (DUF3498); pfam12004"
                     /db_xref="CDD:463427"
     CDS             1..932
                     /gene="raskol"
                     /coded_by="XM_077648264.1:136..2934"
                     /db_xref="GeneID:144114490"
ORIGIN      
        1 mqaaqergsd evrqggsats tpvlggrvpd atatpsrivs ffskrslkqn plkrtksvsk
       61 lerarraeap qprlrgsrsh esllqspggp wglegarlgp lhpslrnhpt qgqawgpapr
      121 gagehwfela prdgpacvya crsrqqclhw larlrqaaqp qrdavrrten glrlwvleak
      181 glapkkryyc elrldgtlya rtsaklkaql cfwgehfefs hlpavgsltv tvfreadrrk
      241 rrdkstalgm vtvplvpara hhvtekwypl vpelkgpptp gakestppsv rlkwryqsle
      301 vqplasyqpf lqylkrdygp lcrllepvlg vrlkeeiatg lvsimhreem aqefltqlvm
      361 adlqdigdqh ltfrgnslat kateaylkll gdgylqetlg pfirdvlasn ldcevdpakv
      421 sggsgalqrq qsllrqrvqq awqritqsah sfpkdlrmlf hgyrqrlggn theeelcdnl
      481 isgsiflrcl cpailspslf glapeypder aarsltliak tiqtlanftr fggkesfmef
      541 mndfvekews tmktflkvis spvsaetnss gseagievpc vvdlglelaq lqmmlreclp
      601 qlegkegeaa lraaldgrpr srgqvlscle anpapapqpr sstlprsala psanmlrevp
      661 ralpdllttm pssqgptavg rvlppqpggc pplafsnpvy rwgglqepqp esltggesgs
      721 sghssaaese asgtsspssp hlwednflga agelagrsre gderlacrsl relganlhda
      781 stnmqyerev aqlkrlmgql qrklslaetq lqqqqqqqhp ltaslgaest eeqqqrrqek
      841 dlqmksiitr litvedelrr eqremqgmir akqqlidaqe rkiqtldaan qrllgalaql
      901 rqhyqdqvql dprllangas spppptyrss sc
//
LOCUS       XP_077504391             730 aa            linear   INV 25-NOV-2025
DEFINITION  ral guanine nucleotide dissociation stimulator-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077504391
VERSION     XP_077504391.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648265.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..730
                     /product="ral guanine nucleotide dissociation
                     stimulator-like isoform X1"
                     /calculated_mol_wt=81233
     Region          98..200
                     /region_name="RasGEF_N"
                     /note="RasGEF N-terminal motif; pfam00618"
                     /db_xref="CDD:459873"
     Site            order(114,164,168..169,172,175..176,214..215,218,225)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:100121"
     Region          271..532
                     /region_name="RasGEF"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     small GTPases. Small GTP-binding proteins of the Ras
                     superfamily function as molecular switches in fundamental
                     events such as signal transduction, cytoskeleton dynamics
                     and intracellular trafficking; cd00155"
                     /db_xref="CDD:238087"
     Site            order(296..298,315..317,319..320,323..324,327,361,
                     364..365,367..369,371..374,376..377,397,400,405..407,410,
                     454..456,459..461,463..465,467..470,485,489,524,527..528)
                     /site_type="other"
                     /note="Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:238087"
     Region          610..703
                     /region_name="RA_RalGDS_like"
                     /note="Ras-associating (RA) domain of RalGDS family;
                     cd00153"
                     /db_xref="CDD:340449"
     Site            order(610,612,636..640,653,656..657)
                     /site_type="other"
                     /note="RA-Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:340449"
     CDS             1..730
                     /gene="Rgl"
                     /coded_by="XM_077648265.1:422..2614"
                     /db_xref="GeneID:144114491"
ORIGIN      
        1 mdsvpthndv lfatissgfq htkmrghsin chghvpwsaa aetikqpswr lwgeeksdga
       61 vytvylkkvr yhpssslsrh dsedsvshle wetvrvrvik agpleaivan laddegglep
      121 tnvnvflaty rsfaspqqvl dafqarfrel knrdgakgls atsqrhhqal vqallvwldm
      181 ypedlyeppq fsvlhqlldl aqaqlepgss lelrarqrlq qflardpeke gpffkpqpwn
      241 vvfvnswpga qaraqmaggp lwlqdlaeen iarqltwmda dlfrrlvphh clsamwrkrg
      301 prdwagtvhn tatatvqqfn avsrrvtstl lwerwdegnr vrllakwigv aqelrllknf
      361 sslkaviaal qsndiyrlss vwarvapdrl qtflelarif ssedhrlscr elmlkegtak
      421 fadtvgdddr qlqkamqrql aagspgtmqg tvpylgiflt dltmldaalp delpggllnf
      481 dkrrrefrvl aqikllqsaa ncyslqpdpa fvawfdtlpm lsdeqsyrls ckvepasspk
      541 rsalssgnvv dgdtasltst ssqpqsggsq eslrrngwtq qrpwqstgnl qsmmsacppp
      601 tpphdpdlkv irvsldptla acqgsptgvn qgttlyksil lsngdhtpav vrnalekhgl
      661 egdpdeyvls qqlpdseivf pssanvfyal stahalnfil rpkksepssp vstkksrkpf
      721 qlklklpsgt
//
LOCUS       XP_077504392             726 aa            linear   INV 25-NOV-2025
DEFINITION  ral guanine nucleotide dissociation stimulator-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077504392
VERSION     XP_077504392.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648266.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..726
                     /product="ral guanine nucleotide dissociation
                     stimulator-like isoform X2"
                     /calculated_mol_wt=80746
     Region          94..196
                     /region_name="RasGEF_N"
                     /note="RasGEF N-terminal motif; pfam00618"
                     /db_xref="CDD:459873"
     Site            order(110,160,164..165,168,171..172,210..211,214,221)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:100121"
     Region          267..528
                     /region_name="RasGEF"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     small GTPases. Small GTP-binding proteins of the Ras
                     superfamily function as molecular switches in fundamental
                     events such as signal transduction, cytoskeleton dynamics
                     and intracellular trafficking; cd00155"
                     /db_xref="CDD:238087"
     Site            order(292..294,311..313,315..316,319..320,323,357,
                     360..361,363..365,367..370,372..373,393,396,401..403,406,
                     450..452,455..457,459..461,463..466,481,485,520,523..524)
                     /site_type="other"
                     /note="Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:238087"
     Region          606..699
                     /region_name="RA_RalGDS_like"
                     /note="Ras-associating (RA) domain of RalGDS family;
                     cd00153"
                     /db_xref="CDD:340449"
     Site            order(606,608,632..636,649,652..653)
                     /site_type="other"
                     /note="RA-Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:340449"
     CDS             1..726
                     /gene="Rgl"
                     /coded_by="XM_077648266.1:527..2707"
                     /db_xref="GeneID:144114491"
ORIGIN      
        1 mnpiidgqat issgfqhtkm rghsinchgh vpwsaaaeti kqpswrlwge eksdgavytv
       61 ylkkvryhps sslsrhdsed svshlewetv rvrvikagpl eaivanladd eggleptnvn
      121 vflatyrsfa spqqvldafq arfrelknrd gakglsatsq rhhqalvqal lvwldmyped
      181 lyeppqfsvl hqlldlaqaq lepgsslelr arqrlqqfla rdpekegpff kpqpwnvvfv
      241 nswpgaqara qmaggplwlq dlaeeniarq ltwmdadlfr rlvphhclsa mwrkrgprdw
      301 agtvhntata tvqqfnavsr rvtstllwer wdegnrvrll akwigvaqel rllknfsslk
      361 aviaalqsnd iyrlssvwar vapdrlqtfl elarifssed hrlscrelml kegtakfadt
      421 vgdddrqlqk amqrqlaags pgtmqgtvpy lgifltdltm ldaalpdelp ggllnfdkrr
      481 refrvlaqik llqsaancys lqpdpafvaw fdtlpmlsde qsyrlsckve passpkrsal
      541 ssgnvvdgdt asltstssqp qsggsqeslr rngwtqqrpw qstgnlqsmm sacppptpph
      601 dpdlkvirvs ldptlaacqg sptgvnqgtt lyksillsng dhtpavvrna lekhglegdp
      661 deyvlsqqlp dseivfpssa nvfyalstah alnfilrpkk sepsspvstk ksrkpfqlkl
      721 klpsgt
//
LOCUS       XP_077504393             726 aa            linear   INV 25-NOV-2025
DEFINITION  ral guanine nucleotide dissociation stimulator-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077504393
VERSION     XP_077504393.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648267.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..726
                     /product="ral guanine nucleotide dissociation
                     stimulator-like isoform X2"
                     /calculated_mol_wt=80746
     Region          94..196
                     /region_name="RasGEF_N"
                     /note="RasGEF N-terminal motif; pfam00618"
                     /db_xref="CDD:459873"
     Site            order(110,160,164..165,168,171..172,210..211,214,221)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:100121"
     Region          267..528
                     /region_name="RasGEF"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     small GTPases. Small GTP-binding proteins of the Ras
                     superfamily function as molecular switches in fundamental
                     events such as signal transduction, cytoskeleton dynamics
                     and intracellular trafficking; cd00155"
                     /db_xref="CDD:238087"
     Site            order(292..294,311..313,315..316,319..320,323,357,
                     360..361,363..365,367..370,372..373,393,396,401..403,406,
                     450..452,455..457,459..461,463..466,481,485,520,523..524)
                     /site_type="other"
                     /note="Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:238087"
     Region          606..699
                     /region_name="RA_RalGDS_like"
                     /note="Ras-associating (RA) domain of RalGDS family;
                     cd00153"
                     /db_xref="CDD:340449"
     Site            order(606,608,632..636,649,652..653)
                     /site_type="other"
                     /note="RA-Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:340449"
     CDS             1..726
                     /gene="Rgl"
                     /coded_by="XM_077648267.1:215..2395"
                     /db_xref="GeneID:144114491"
ORIGIN      
        1 mnpiidgqat issgfqhtkm rghsinchgh vpwsaaaeti kqpswrlwge eksdgavytv
       61 ylkkvryhps sslsrhdsed svshlewetv rvrvikagpl eaivanladd eggleptnvn
      121 vflatyrsfa spqqvldafq arfrelknrd gakglsatsq rhhqalvqal lvwldmyped
      181 lyeppqfsvl hqlldlaqaq lepgsslelr arqrlqqfla rdpekegpff kpqpwnvvfv
      241 nswpgaqara qmaggplwlq dlaeeniarq ltwmdadlfr rlvphhclsa mwrkrgprdw
      301 agtvhntata tvqqfnavsr rvtstllwer wdegnrvrll akwigvaqel rllknfsslk
      361 aviaalqsnd iyrlssvwar vapdrlqtfl elarifssed hrlscrelml kegtakfadt
      421 vgdddrqlqk amqrqlaags pgtmqgtvpy lgifltdltm ldaalpdelp ggllnfdkrr
      481 refrvlaqik llqsaancys lqpdpafvaw fdtlpmlsde qsyrlsckve passpkrsal
      541 ssgnvvdgdt asltstssqp qsggsqeslr rngwtqqrpw qstgnlqsmm sacppptpph
      601 dpdlkvirvs ldptlaacqg sptgvnqgtt lyksillsng dhtpavvrna lekhglegdp
      661 deyvlsqqlp dseivfpssa nvfyalstah alnfilrpkk sepsspvstk ksrkpfqlkl
      721 klpsgt
//
LOCUS       XP_077504394             726 aa            linear   INV 25-NOV-2025
DEFINITION  ral guanine nucleotide dissociation stimulator-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077504394
VERSION     XP_077504394.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648268.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..726
                     /product="ral guanine nucleotide dissociation
                     stimulator-like isoform X2"
                     /calculated_mol_wt=80746
     Region          94..196
                     /region_name="RasGEF_N"
                     /note="RasGEF N-terminal motif; pfam00618"
                     /db_xref="CDD:459873"
     Site            order(110,160,164..165,168,171..172,210..211,214,221)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:100121"
     Region          267..528
                     /region_name="RasGEF"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     small GTPases. Small GTP-binding proteins of the Ras
                     superfamily function as molecular switches in fundamental
                     events such as signal transduction, cytoskeleton dynamics
                     and intracellular trafficking; cd00155"
                     /db_xref="CDD:238087"
     Site            order(292..294,311..313,315..316,319..320,323,357,
                     360..361,363..365,367..370,372..373,393,396,401..403,406,
                     450..452,455..457,459..461,463..466,481,485,520,523..524)
                     /site_type="other"
                     /note="Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:238087"
     Region          606..699
                     /region_name="RA_RalGDS_like"
                     /note="Ras-associating (RA) domain of RalGDS family;
                     cd00153"
                     /db_xref="CDD:340449"
     Site            order(606,608,632..636,649,652..653)
                     /site_type="other"
                     /note="RA-Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:340449"
     CDS             1..726
                     /gene="Rgl"
                     /coded_by="XM_077648268.1:178..2358"
                     /db_xref="GeneID:144114491"
ORIGIN      
        1 mnpiidgqat issgfqhtkm rghsinchgh vpwsaaaeti kqpswrlwge eksdgavytv
       61 ylkkvryhps sslsrhdsed svshlewetv rvrvikagpl eaivanladd eggleptnvn
      121 vflatyrsfa spqqvldafq arfrelknrd gakglsatsq rhhqalvqal lvwldmyped
      181 lyeppqfsvl hqlldlaqaq lepgsslelr arqrlqqfla rdpekegpff kpqpwnvvfv
      241 nswpgaqara qmaggplwlq dlaeeniarq ltwmdadlfr rlvphhclsa mwrkrgprdw
      301 agtvhntata tvqqfnavsr rvtstllwer wdegnrvrll akwigvaqel rllknfsslk
      361 aviaalqsnd iyrlssvwar vapdrlqtfl elarifssed hrlscrelml kegtakfadt
      421 vgdddrqlqk amqrqlaags pgtmqgtvpy lgifltdltm ldaalpdelp ggllnfdkrr
      481 refrvlaqik llqsaancys lqpdpafvaw fdtlpmlsde qsyrlsckve passpkrsal
      541 ssgnvvdgdt asltstssqp qsggsqeslr rngwtqqrpw qstgnlqsmm sacppptpph
      601 dpdlkvirvs ldptlaacqg sptgvnqgtt lyksillsng dhtpavvrna lekhglegdp
      661 deyvlsqqlp dseivfpssa nvfyalstah alnfilrpkk sepsspvstk ksrkpfqlkl
      721 klpsgt
//
LOCUS       XP_077504395             707 aa            linear   INV 25-NOV-2025
DEFINITION  ral guanine nucleotide dissociation stimulator-like isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077504395
VERSION     XP_077504395.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648269.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..707
                     /product="ral guanine nucleotide dissociation
                     stimulator-like isoform X3"
                     /calculated_mol_wt=78719
     Region          75..177
                     /region_name="RasGEF_N"
                     /note="RasGEF N-terminal motif; pfam00618"
                     /db_xref="CDD:459873"
     Site            order(91,141,145..146,149,152..153,191..192,195,202)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:100121"
     Region          248..509
                     /region_name="RasGEF"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     small GTPases. Small GTP-binding proteins of the Ras
                     superfamily function as molecular switches in fundamental
                     events such as signal transduction, cytoskeleton dynamics
                     and intracellular trafficking; cd00155"
                     /db_xref="CDD:238087"
     Site            order(273..275,292..294,296..297,300..301,304,338,
                     341..342,344..346,348..351,353..354,374,377,382..384,387,
                     431..433,436..438,440..442,444..447,462,466,501,504..505)
                     /site_type="other"
                     /note="Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:238087"
     Region          587..680
                     /region_name="RA_RalGDS_like"
                     /note="Ras-associating (RA) domain of RalGDS family;
                     cd00153"
                     /db_xref="CDD:340449"
     Site            order(587,589,613..617,630,633..634)
                     /site_type="other"
                     /note="RA-Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:340449"
     CDS             1..707
                     /gene="Rgl"
                     /coded_by="XM_077648269.1:106..2229"
                     /db_xref="GeneID:144114491"
ORIGIN      
        1 mrghsinchg hvpwsaaaet ikqpswrlwg eeksdgavyt vylkkvryhp ssslsrhdse
       61 dsvshlewet vrvrvikagp leaivanlad deggleptnv nvflatyrsf aspqqvldaf
      121 qarfrelknr dgakglsats qrhhqalvqa llvwldmype dlyeppqfsv lhqlldlaqa
      181 qlepgsslel rarqrlqqfl ardpekegpf fkpqpwnvvf vnswpgaqar aqmaggplwl
      241 qdlaeeniar qltwmdadlf rrlvphhcls amwrkrgprd wagtvhntat atvqqfnavs
      301 rrvtstllwe rwdegnrvrl lakwigvaqe lrllknfssl kaviaalqsn diyrlssvwa
      361 rvapdrlqtf lelarifsse dhrlscrelm lkegtakfad tvgdddrqlq kamqrqlaag
      421 spgtmqgtvp ylgifltdlt mldaalpdel pggllnfdkr rrefrvlaqi kllqsaancy
      481 slqpdpafva wfdtlpmlsd eqsyrlsckv epasspkrsa lssgnvvdgd tasltstssq
      541 pqsggsqesl rrngwtqqrp wqstgnlqsm msacppptpp hdpdlkvirv sldptlaacq
      601 gsptgvnqgt tlyksillsn gdhtpavvrn alekhglegd pdeyvlsqql pdseivfpss
      661 anvfyalsta halnfilrpk ksepsspvst kksrkpfqlk lklpsgt
//
LOCUS       XP_077504396             701 aa            linear   INV 25-NOV-2025
DEFINITION  ral guanine nucleotide dissociation stimulator-like isoform X4
            [Amblyomma americanum].
ACCESSION   XP_077504396
VERSION     XP_077504396.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648270.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..701
                     /product="ral guanine nucleotide dissociation
                     stimulator-like isoform X4"
                     /calculated_mol_wt=78143
     Region          98..200
                     /region_name="RasGEF_N"
                     /note="RasGEF N-terminal motif; pfam00618"
                     /db_xref="CDD:459873"
     Site            order(114,164,168..169,172,175..176,214..215,218,225)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:100121"
     Region          271..503
                     /region_name="RasGEF"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     small GTPases. Small GTP-binding proteins of the Ras
                     superfamily function as molecular switches in fundamental
                     events such as signal transduction, cytoskeleton dynamics
                     and intracellular trafficking; cd00155"
                     /db_xref="CDD:238087"
     Site            order(296..298,315..317,319..320,323..324,327,361,
                     364..365,367..369,371..374,376..377,397,400,405..407,410,
                     425..427,430..432,434..436,438..441,456,460,495,498..499)
                     /site_type="other"
                     /note="Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:238087"
     Region          581..674
                     /region_name="RA_RalGDS_like"
                     /note="Ras-associating (RA) domain of RalGDS family;
                     cd00153"
                     /db_xref="CDD:340449"
     Site            order(581,583,607..611,624,627..628)
                     /site_type="other"
                     /note="RA-Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:340449"
     CDS             1..701
                     /gene="Rgl"
                     /coded_by="XM_077648270.1:422..2527"
                     /db_xref="GeneID:144114491"
ORIGIN      
        1 mdsvpthndv lfatissgfq htkmrghsin chghvpwsaa aetikqpswr lwgeeksdga
       61 vytvylkkvr yhpssslsrh dsedsvshle wetvrvrvik agpleaivan laddegglep
      121 tnvnvflaty rsfaspqqvl dafqarfrel knrdgakgls atsqrhhqal vqallvwldm
      181 ypedlyeppq fsvlhqlldl aqaqlepgss lelrarqrlq qflardpeke gpffkpqpwn
      241 vvfvnswpga qaraqmaggp lwlqdlaeen iarqltwmda dlfrrlvphh clsamwrkrg
      301 prdwagtvhn tatatvqqfn avsrrvtstl lwerwdegnr vrllakwigv aqelrllknf
      361 sslkaviaal qsndiyrlss vwarvapdrl qtflelarif ssedhrlscr elmlkpgtmq
      421 gtvpylgifl tdltmldaal pdelpgglln fdkrrrefrv laqikllqsa ancyslqpdp
      481 afvawfdtlp mlsdeqsyrl sckvepassp krsalssgnv vdgdtaslts tssqpqsggs
      541 qeslrrngwt qqrpwqstgn lqsmmsacpp ptpphdpdlk virvsldptl aacqgsptgv
      601 nqgttlyksi llsngdhtpa vvrnalekhg legdpdeyvl sqqlpdseiv fpssanvfya
      661 lstahalnfi lrpkksepss pvstkksrkp fqlklklpsg t
//
LOCUS       XP_077504397             693 aa            linear   INV 25-NOV-2025
DEFINITION  ral guanine nucleotide dissociation stimulator-like isoform X5
            [Amblyomma americanum].
ACCESSION   XP_077504397
VERSION     XP_077504397.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648271.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..693
                     /product="ral guanine nucleotide dissociation
                     stimulator-like isoform X5"
                     /calculated_mol_wt=77203
     Region          61..163
                     /region_name="RasGEF_N"
                     /note="RasGEF N-terminal motif; pfam00618"
                     /db_xref="CDD:459873"
     Site            order(77,127,131..132,135,138..139,177..178,181,188)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:100121"
     Region          234..495
                     /region_name="RasGEF"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     small GTPases. Small GTP-binding proteins of the Ras
                     superfamily function as molecular switches in fundamental
                     events such as signal transduction, cytoskeleton dynamics
                     and intracellular trafficking; cd00155"
                     /db_xref="CDD:238087"
     Site            order(259..261,278..280,282..283,286..287,290,324,
                     327..328,330..332,334..337,339..340,360,363,368..370,373,
                     417..419,422..424,426..428,430..433,448,452,487,490..491)
                     /site_type="other"
                     /note="Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:238087"
     Region          573..666
                     /region_name="RA_RalGDS_like"
                     /note="Ras-associating (RA) domain of RalGDS family;
                     cd00153"
                     /db_xref="CDD:340449"
     Site            order(573,575,599..603,616,619..620)
                     /site_type="other"
                     /note="RA-Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:340449"
     CDS             1..693
                     /gene="Rgl"
                     /coded_by="XM_077648271.1:564..2645"
                     /db_xref="GeneID:144114491"
ORIGIN      
        1 mnpiidgqqp swrlwgeeks dgavytvylk kvryhpsssl srhdsedsvs hlewetvrvr
       61 vikagpleai vanladdegg leptnvnvfl atyrsfaspq qvldafqarf relknrdgak
      121 glsatsqrhh qalvqallvw ldmypedlye ppqfsvlhql ldlaqaqlep gsslelrarq
      181 rlqqflardp ekegpffkpq pwnvvfvnsw pgaqaraqma ggplwlqdla eeniarqltw
      241 mdadlfrrlv phhclsamwr krgprdwagt vhntatatvq qfnavsrrvt stllwerwde
      301 gnrvrllakw igvaqelrll knfsslkavi aalqsndiyr lssvwarvap drlqtflela
      361 rifssedhrl screlmlkeg takfadtvgd ddrqlqkamq rqlaagspgt mqgtvpylgi
      421 fltdltmlda alpdelpggl lnfdkrrref rvlaqikllq saancyslqp dpafvawfdt
      481 lpmlsdeqsy rlsckvepas spkrsalssg nvvdgdtasl tstssqpqsg gsqeslrrng
      541 wtqqrpwqst gnlqsmmsac ppptpphdpd lkvirvsldp tlaacqgspt gvnqgttlyk
      601 sillsngdht pavvrnalek hglegdpdey vlsqqlpdse ivfpssanvf yalstahaln
      661 filrpkksep sspvstkksr kpfqlklklp sgt
//
LOCUS       XP_077504398             693 aa            linear   INV 25-NOV-2025
DEFINITION  ral guanine nucleotide dissociation stimulator-like isoform X5
            [Amblyomma americanum].
ACCESSION   XP_077504398
VERSION     XP_077504398.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648272.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..693
                     /product="ral guanine nucleotide dissociation
                     stimulator-like isoform X5"
                     /calculated_mol_wt=77203
     Region          61..163
                     /region_name="RasGEF_N"
                     /note="RasGEF N-terminal motif; pfam00618"
                     /db_xref="CDD:459873"
     Site            order(77,127,131..132,135,138..139,177..178,181,188)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:100121"
     Region          234..495
                     /region_name="RasGEF"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     small GTPases. Small GTP-binding proteins of the Ras
                     superfamily function as molecular switches in fundamental
                     events such as signal transduction, cytoskeleton dynamics
                     and intracellular trafficking; cd00155"
                     /db_xref="CDD:238087"
     Site            order(259..261,278..280,282..283,286..287,290,324,
                     327..328,330..332,334..337,339..340,360,363,368..370,373,
                     417..419,422..424,426..428,430..433,448,452,487,490..491)
                     /site_type="other"
                     /note="Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:238087"
     Region          573..666
                     /region_name="RA_RalGDS_like"
                     /note="Ras-associating (RA) domain of RalGDS family;
                     cd00153"
                     /db_xref="CDD:340449"
     Site            order(573,575,599..603,616,619..620)
                     /site_type="other"
                     /note="RA-Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:340449"
     CDS             1..693
                     /gene="Rgl"
                     /coded_by="XM_077648272.1:401..2482"
                     /db_xref="GeneID:144114491"
ORIGIN      
        1 mnpiidgqqp swrlwgeeks dgavytvylk kvryhpsssl srhdsedsvs hlewetvrvr
       61 vikagpleai vanladdegg leptnvnvfl atyrsfaspq qvldafqarf relknrdgak
      121 glsatsqrhh qalvqallvw ldmypedlye ppqfsvlhql ldlaqaqlep gsslelrarq
      181 rlqqflardp ekegpffkpq pwnvvfvnsw pgaqaraqma ggplwlqdla eeniarqltw
      241 mdadlfrrlv phhclsamwr krgprdwagt vhntatatvq qfnavsrrvt stllwerwde
      301 gnrvrllakw igvaqelrll knfsslkavi aalqsndiyr lssvwarvap drlqtflela
      361 rifssedhrl screlmlkeg takfadtvgd ddrqlqkamq rqlaagspgt mqgtvpylgi
      421 fltdltmlda alpdelpggl lnfdkrrref rvlaqikllq saancyslqp dpafvawfdt
      481 lpmlsdeqsy rlsckvepas spkrsalssg nvvdgdtasl tstssqpqsg gsqeslrrng
      541 wtqqrpwqst gnlqsmmsac ppptpphdpd lkvirvsldp tlaacqgspt gvnqgttlyk
      601 sillsngdht pavvrnalek hglegdpdey vlsqqlpdse ivfpssanvf yalstahaln
      661 filrpkksep sspvstkksr kpfqlklklp sgt
//
LOCUS       XP_077504399             693 aa            linear   INV 25-NOV-2025
DEFINITION  ral guanine nucleotide dissociation stimulator-like isoform X5
            [Amblyomma americanum].
ACCESSION   XP_077504399
VERSION     XP_077504399.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648273.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..693
                     /product="ral guanine nucleotide dissociation
                     stimulator-like isoform X5"
                     /calculated_mol_wt=77203
     Region          61..163
                     /region_name="RasGEF_N"
                     /note="RasGEF N-terminal motif; pfam00618"
                     /db_xref="CDD:459873"
     Site            order(77,127,131..132,135,138..139,177..178,181,188)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:100121"
     Region          234..495
                     /region_name="RasGEF"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     small GTPases. Small GTP-binding proteins of the Ras
                     superfamily function as molecular switches in fundamental
                     events such as signal transduction, cytoskeleton dynamics
                     and intracellular trafficking; cd00155"
                     /db_xref="CDD:238087"
     Site            order(259..261,278..280,282..283,286..287,290,324,
                     327..328,330..332,334..337,339..340,360,363,368..370,373,
                     417..419,422..424,426..428,430..433,448,452,487,490..491)
                     /site_type="other"
                     /note="Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:238087"
     Region          573..666
                     /region_name="RA_RalGDS_like"
                     /note="Ras-associating (RA) domain of RalGDS family;
                     cd00153"
                     /db_xref="CDD:340449"
     Site            order(573,575,599..603,616,619..620)
                     /site_type="other"
                     /note="RA-Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:340449"
     CDS             1..693
                     /gene="Rgl"
                     /coded_by="XM_077648273.1:193..2274"
                     /db_xref="GeneID:144114491"
ORIGIN      
        1 mnpiidgqqp swrlwgeeks dgavytvylk kvryhpsssl srhdsedsvs hlewetvrvr
       61 vikagpleai vanladdegg leptnvnvfl atyrsfaspq qvldafqarf relknrdgak
      121 glsatsqrhh qalvqallvw ldmypedlye ppqfsvlhql ldlaqaqlep gsslelrarq
      181 rlqqflardp ekegpffkpq pwnvvfvnsw pgaqaraqma ggplwlqdla eeniarqltw
      241 mdadlfrrlv phhclsamwr krgprdwagt vhntatatvq qfnavsrrvt stllwerwde
      301 gnrvrllakw igvaqelrll knfsslkavi aalqsndiyr lssvwarvap drlqtflela
      361 rifssedhrl screlmlkeg takfadtvgd ddrqlqkamq rqlaagspgt mqgtvpylgi
      421 fltdltmlda alpdelpggl lnfdkrrref rvlaqikllq saancyslqp dpafvawfdt
      481 lpmlsdeqsy rlsckvepas spkrsalssg nvvdgdtasl tstssqpqsg gsqeslrrng
      541 wtqqrpwqst gnlqsmmsac ppptpphdpd lkvirvsldp tlaacqgspt gvnqgttlyk
      601 sillsngdht pavvrnalek hglegdpdey vlsqqlpdse ivfpssanvf yalstahaln
      661 filrpkksep sspvstkksr kpfqlklklp sgt
//
LOCUS       XP_077504401             539 aa            linear   INV 25-NOV-2025
DEFINITION  organic cation transporter protein-like [Amblyomma americanum].
ACCESSION   XP_077504401
VERSION     XP_077504401.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648275.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..539
                     /product="organic cation transporter protein-like"
                     /calculated_mol_wt=58935
     Region          76..483
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..539
                     /gene="LOC144114493"
                     /coded_by="XM_077648275.1:429..2048"
                     /db_xref="GeneID:144114493"
ORIGIN      
        1 madatpdlgr whipvilatf yrgvpsamiv mvinfaaprl hywcrdaile degtegpain
       61 asacwlhvng srvrceawef dhdtftstiv edwslvcdra wmrsvsqsvc lmgmffgnfl
      121 fsplsdrygr ritvlitsll mlifgvmtaf swsltvfnit rfvaslgvgg iqsttatlfv
      181 etvpsryrll fgatygagwi cgqlglvata wfvrrwfmlq ltvsllslpl vlnwwflpes
      241 prwllsqgri aeaskelrra aalngslgng elaappatrt atgetestkk gfnfvnllks
      301 pklrrftmvf fitsfahrlr yfhltyssal lsgdlfvnya ivhvmelpgk lllgalavkf
      361 lwrrptlagc fivaaaallg slavthdspw arvglnavsm lsisgaadll svfaaevfpt
      421 clrtvgmgaa ymaacvgatv aplmvvegap agvnegilcf lqllavlsll lvpetlhrsl
      481 pdtieeaesg ckekdkpnke kvleglasqe etakpppped tspcifdess apwseqtrl
//
LOCUS       XP_077504402             539 aa            linear   INV 25-NOV-2025
DEFINITION  organic cation transporter protein-like [Amblyomma americanum].
ACCESSION   XP_077504402
VERSION     XP_077504402.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648276.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..539
                     /product="organic cation transporter protein-like"
                     /calculated_mol_wt=58935
     Region          76..483
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..539
                     /gene="LOC144114493"
                     /coded_by="XM_077648276.1:186..1805"
                     /db_xref="GeneID:144114493"
ORIGIN      
        1 madatpdlgr whipvilatf yrgvpsamiv mvinfaaprl hywcrdaile degtegpain
       61 asacwlhvng srvrceawef dhdtftstiv edwslvcdra wmrsvsqsvc lmgmffgnfl
      121 fsplsdrygr ritvlitsll mlifgvmtaf swsltvfnit rfvaslgvgg iqsttatlfv
      181 etvpsryrll fgatygagwi cgqlglvata wfvrrwfmlq ltvsllslpl vlnwwflpes
      241 prwllsqgri aeaskelrra aalngslgng elaappatrt atgetestkk gfnfvnllks
      301 pklrrftmvf fitsfahrlr yfhltyssal lsgdlfvnya ivhvmelpgk lllgalavkf
      361 lwrrptlagc fivaaaallg slavthdspw arvglnavsm lsisgaadll svfaaevfpt
      421 clrtvgmgaa ymaacvgatv aplmvvegap agvnegilcf lqllavlsll lvpetlhrsl
      481 pdtieeaesg ckekdkpnke kvleglasqe etakpppped tspcifdess apwseqtrl
//
LOCUS       XP_077504403             539 aa            linear   INV 25-NOV-2025
DEFINITION  organic cation transporter protein-like [Amblyomma americanum].
ACCESSION   XP_077504403
VERSION     XP_077504403.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648277.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..539
                     /product="organic cation transporter protein-like"
                     /calculated_mol_wt=58935
     Region          76..483
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..539
                     /gene="LOC144114493"
                     /coded_by="XM_077648277.1:271..1890"
                     /db_xref="GeneID:144114493"
ORIGIN      
        1 madatpdlgr whipvilatf yrgvpsamiv mvinfaaprl hywcrdaile degtegpain
       61 asacwlhvng srvrceawef dhdtftstiv edwslvcdra wmrsvsqsvc lmgmffgnfl
      121 fsplsdrygr ritvlitsll mlifgvmtaf swsltvfnit rfvaslgvgg iqsttatlfv
      181 etvpsryrll fgatygagwi cgqlglvata wfvrrwfmlq ltvsllslpl vlnwwflpes
      241 prwllsqgri aeaskelrra aalngslgng elaappatrt atgetestkk gfnfvnllks
      301 pklrrftmvf fitsfahrlr yfhltyssal lsgdlfvnya ivhvmelpgk lllgalavkf
      361 lwrrptlagc fivaaaallg slavthdspw arvglnavsm lsisgaadll svfaaevfpt
      421 clrtvgmgaa ymaacvgatv aplmvvegap agvnegilcf lqllavlsll lvpetlhrsl
      481 pdtieeaesg ckekdkpnke kvleglasqe etakpppped tspcifdess apwseqtrl
//
LOCUS       XP_077504404             302 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114494 [Amblyomma americanum].
ACCESSION   XP_077504404
VERSION     XP_077504404.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648278.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..302
                     /product="uncharacterized protein LOC144114494"
                     /calculated_mol_wt=32309
     CDS             1..302
                     /gene="LOC144114494"
                     /coded_by="XM_077648278.1:422..1330"
                     /db_xref="GeneID:144114494"
ORIGIN      
        1 mpisdeasvp vvsyrqkyrn lkrklkflly enecfqeelr kaqrkllkvt rdksflldqi
       61 lqyesidsss sdsdatvssd sdselrvepp pppfpavkkk kptvtpvesa nvhhlpvasi
      121 siaavpsaap scsipiktei aplpgstvst algvlptlaa kstvlmpssa slnptvekkk
      181 kvartskppp atvsapphpv skpllvtgvn krmvevvdal svsrldghmt seeverhlea
      241 rqalrdllpe kapltvpael fsneppadll dsgppllvva psamasadae geedelitgm
      301 pd
//
LOCUS       XP_077504405             206 aa            linear   INV 25-NOV-2025
DEFINITION  mitotic arrest deficient 2 [Amblyomma americanum].
ACCESSION   XP_077504405
VERSION     XP_077504405.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648279.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..206
                     /product="mitotic arrest deficient 2"
                     /calculated_mol_wt=23123
     Region          17..192
                     /region_name="HORMA"
                     /note="HORMA domain; pfam02301"
                     /db_xref="CDD:460526"
     CDS             1..206
                     /gene="mad2"
                     /coded_by="XM_077648279.1:132..752"
                     /db_xref="GeneID:144114495"
ORIGIN      
        1 mqgsatvtqn aitlkgsaqi vadffnygin silyqrgvyp pdmfttvqky glailvttdk
       61 glqdyldnil tqlkewleak avhrvilvis tcstketler wefqiqaeee ttniskpkek
      121 elseiqneik svirqivasv tylpiletac afdlliyadk nvkvpkkwed sapllipnce
      181 tvrlksfstt vhsvdtavsy knilkm
//
LOCUS       XP_077504406             567 aa            linear   INV 25-NOV-2025
DEFINITION  prolyl 4-hydroxylase subunit alpha-1 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504406
VERSION     XP_077504406.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648280.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..567
                     /product="prolyl 4-hydroxylase subunit alpha-1 isoform X1"
                     /calculated_mol_wt=64571
     Region          32..164
                     /region_name="P4Ha_N"
                     /note="Prolyl 4-Hydroxylase alpha-subunit, N-terminal
                     region; pfam08336"
                     /db_xref="CDD:462433"
     Region          362..551
                     /region_name="P4Hc"
                     /note="Prolyl 4-hydroxylase alpha subunit homologues;
                     smart00702"
                     /db_xref="CDD:214780"
     CDS             1..567
                     /gene="PH4alphaEFB"
                     /coded_by="XM_077648280.1:237..1940"
                     /db_xref="GeneID:144114496"
ORIGIN      
        1 magvqkffmh avlitcictl lstqvqsevf taladlehll rtegavvqtl qryldaeegr
       61 lekikklrqe fnqlhkaasr dgdefisnpv nafllvkklt adwkavarlm ldtegkamve
      121 nithsghlrf pdeedltgaa fallrlqdty rldtaslakg hiqgvtrpss elsagdcfel
      181 grqsynnedh yhtvlwmqea ldrvdeevdk tadraeiley lafstyqqgn vhhalkltnd
      241 llaaapdhpr apgnkryyed mlakreqymk rgddgdmsen dsvtlkkrsp lpdadsergi
      301 yerlcrgekf pplyhdrelt cqyrtnnrpy lllqpakeev mfpkprivvy hdvlsehemn
      361 viktlaqprl rratvqnyks geletanyri sksawlknee hgviarvsrr ieditgltad
      421 taeelqvvny gigghyephf dfarreekna fqslgtgnri atwlnykkgk qklplhlsng
      481 nrvstwlnym sdvpaggatv fpqlrltlwp kkgaaafwfn lhrsgegdml trhaacpvla
      541 gskwvsnkwf hergqeflrp cgtrinq
//
LOCUS       XP_077504407             544 aa            linear   INV 25-NOV-2025
DEFINITION  prolyl 4-hydroxylase subunit alpha-1 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504407
VERSION     XP_077504407.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648281.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..544
                     /product="prolyl 4-hydroxylase subunit alpha-1 isoform X2"
                     /calculated_mol_wt=61907
     Region          32..164
                     /region_name="P4Ha_N"
                     /note="Prolyl 4-Hydroxylase alpha-subunit, N-terminal
                     region; pfam08336"
                     /db_xref="CDD:462433"
     Region          362..528
                     /region_name="P4Hc"
                     /note="Prolyl 4-hydroxylase alpha subunit homologues;
                     smart00702"
                     /db_xref="CDD:214780"
     CDS             1..544
                     /gene="PH4alphaEFB"
                     /coded_by="XM_077648281.1:237..1871"
                     /db_xref="GeneID:144114496"
ORIGIN      
        1 magvqkffmh avlitcictl lstqvqsevf taladlehll rtegavvqtl qryldaeegr
       61 lekikklrqe fnqlhkaasr dgdefisnpv nafllvkklt adwkavarlm ldtegkamve
      121 nithsghlrf pdeedltgaa fallrlqdty rldtaslakg hiqgvtrpss elsagdcfel
      181 grqsynnedh yhtvlwmqea ldrvdeevdk tadraeiley lafstyqqgn vhhalkltnd
      241 llaaapdhpr apgnkryyed mlakreqymk rgddgdmsen dsvtlkkrsp lpdadsergi
      301 yerlcrgekf pplyhdrelt cqyrtnnrpy lllqpakeev mfpkprivvy hdvlsehemn
      361 viktlaqprl rratvqnyks geletanyri sksawlknee hgviarvsrr ieditgltad
      421 taeelqvvny gigghyephf dfarreekna fqslgtgnri atwlnymsdv paggatvfpq
      481 lrltlwpkkg aaafwfnlhr sgegdmltrh aacpvlagsk wvsnkwfher gqeflrpcgt
      541 rinq
//
LOCUS       XP_077504408            1121 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 2-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504408
VERSION     XP_077504408.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648282.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1121
                     /product="endothelin-converting enzyme 2-like isoform X1"
                     /calculated_mol_wt=123276
     Region          489..1107
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..1121
                     /gene="LOC144114497"
                     /coded_by="XM_077648282.1:298..3663"
                     /db_xref="GeneID:144114497"
ORIGIN      
        1 mddydsraeq aeaerlrseq kripeprark glasskndal staqkrrgda eaqtsstrps
       61 kppkplkrki rpaakntdvc daftaprdrh ekqvfhantn psavviqpwc srddstsgsl
      121 rdirgrarvt dedlccsaam egtsdscqgk pryafalgrk asvganatsk raatteaksr
      181 kgrppdvaag rvpvtarppe pnttstkrrg agavlsgssg ggdnlktptr netpaqhaad
      241 etsttdpecg iylprirags sddtteepaa kshslnkrra ssrapghrat diaitssrfk
      301 vkdkrhrrty asntaapsws ttaeasnsle rdlenleyna dadegaqktt alpcvessvk
      361 etpvstvsdn sliettasgd varrpnnsqq lkkellvfha iilvalsgaa alaiafypga
      421 vkatssfpvi ienglpvsvs svptvttsqq pldapeqvvh keplydvcst epcqrdgayi
      481 raliskdldp cdnfykfvcg rwkgindsss pdkelvlkle sevhhvlasp lppsvqdsdi
      541 lsplkrvmts cmnavsgtnv dpvldllaaa dlegfpftpp irssisawkt sakvflltga
      601 sallklgval hpfepdrdii tvdepdpliv gpertttayr rlmqasmnal gkrfvpaays
      661 mevvaftqql daavasgesq rseerlhrvv klkdetelqi yllevlgdlq lppdeedrdv
      721 mllspdfsra lidllrktdv hvvlnflcvh vlvrssvfvs madpdlewav saqlfnhpvh
      781 hiprwrlcvr aaaqtlpdlf lyashqvmga asntlhgvld nvlkqvithi etlifldgqt
      841 rvlakrilsd vrftvglppw lsdmskvsay vaklplfsnr gnflkwfsev nemvqtsalr
      901 rssrefwras pfetdcwydl qlktvfipvl lanwtlyrnd eldnlqlprq ghraarcllr
      961 mlfegvssff nreiltrsfw ssaaktslrr aqlclqeqah ipasmtlsll edhtalriiq
     1021 elyqsnvhis saatasrglr dvrlpgaedf tadhlffiyy algfcanslr eedearlvni
     1081 plrgslmfhe vygcrptskm gpldkctawv aaarqsaplr s
//
LOCUS       XP_077504409             982 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 2-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504409
VERSION     XP_077504409.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648283.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..982
                     /product="endothelin-converting enzyme 2-like isoform X2"
                     /calculated_mol_wt=107833
     Region          350..968
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..982
                     /gene="LOC144114497"
                     /coded_by="XM_077648283.1:515..3463"
                     /db_xref="GeneID:144114497"
ORIGIN      
        1 megtsdscqg kpryafalgr kasvganats kraatteaks rkgrppdvaa grvpvtarpp
       61 epnttstkrr gagavlsgss gggdnlktpt rnetpaqhaa detsttdpec giylprirag
      121 ssddtteepa akshslnkrr assrapghra tdiaitssrf kvkdkrhrrt yasntaapsw
      181 sttaeasnsl erdlenleyn adadegaqkt talpcvessv ketpvstvsd nsliettasg
      241 dvarrpnnsq qlkkellvfh aiilvalsga aalaiafypg avkatssfpv iienglpvsv
      301 ssvptvttsq qpldapeqvv hkeplydvcs tepcqrdgay iraliskdld pcdnfykfvc
      361 grwkgindss spdkelvlkl esevhhvlas plppsvqdsd ilsplkrvmt scmnavsgtn
      421 vdpvldllaa adlegfpftp pirssisawk tsakvflltg asallklgva lhpfepdrdi
      481 itvdepdpli vgpertttay rrlmqasmna lgkrfvpaay smevvaftqq ldaavasges
      541 qrseerlhrv vklkdetelq iyllevlgdl qlppdeedrd vmllspdfsr alidllrktd
      601 vhvvlnflcv hvlvrssvfv smadpdlewa vsaqlfnhpv hhiprwrlcv raaaqtlpdl
      661 flyashqvmg aasntlhgvl dnvlkqvith ietlifldgq trvlakrils dvrftvglpp
      721 wlsdmskvsa yvaklplfsn rgnflkwfse vnemvqtsal rrssrefwra spfetdcwyd
      781 lqlktvfipv llanwtlyrn deldnlqlpr qghraarcll rmlfegvssf fnreiltrsf
      841 wssaaktslr raqlclqeqa hipasmtlsl ledhtalrii qelyqsnvhi ssaatasrgl
      901 rdvrlpgaed ftadhlffiy yalgfcansl reedearlvn iplrgslmfh evygcrptsk
      961 mgpldkctaw vaaarqsapl rs
//
LOCUS       XP_077504410             691 aa            linear   INV 25-NOV-2025
DEFINITION  neutral ceramidase [Amblyomma americanum].
ACCESSION   XP_077504410
VERSION     XP_077504410.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648284.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..691
                     /product="neutral ceramidase"
                     /calculated_mol_wt=76873
     Region          3..689
                     /region_name="PTZ00487"
                     /note="ceramidase; Provisional"
                     /db_xref="CDD:240437"
     CDS             1..691
                     /gene="CDase"
                     /coded_by="XM_077648284.1:242..2317"
                     /db_xref="GeneID:144114498"
ORIGIN      
        1 mwnyvflcll flfrliggld reeyeigvgi aditgpaaei gmlgyaktgq sssgihfrvy
       61 srtfiikhgs srvvivnvds gmigdivkie vvkrlqekyg pamyaennvl ltaththasv
      121 ggfmqyllyn ihtqgfirqv anvqiegicr sidqaheslr rgyiywnege llhsninrsp
      181 saydanppee rakyqnntdt kmlllkftdl qnnpigminw fsvhptsmnn snllisgdnk
      241 gyaelkfeak mnnnlpgkgp fvaafaqsne gdvspnlngp rcidtglpcd fqkstcngrt
      301 ekciafgpgk dmfestkilg erqlqkaldl fhsasrklsg pvrfayqrvn mgkyevgdne
      361 adpittctaa lgysfaagtt dgpgqfdftq sttkstpfwn fvrdfiaaps keaikchhpk
      421 pillpvgemn fpypwvasii ptqilrigql yiiaapgeft tmsgrrlrat vervvrsryp
      481 dsmvvlagls ntythyvaty eeyqvqryeg astlygphtl layqkqfegl amsltsnvtl
      541 pagpplpnll skqisfktgv vfdgaplgkv fgqvlsdakp eytrgervfv tfvsghprnn
      601 lmlestyltv erlnddgesw tvvatdgnwe trfywrrtsa ilghseatvm weipldiapg
      661 iyrirnfghs knilqvvtpy egsssrfkvn p
//
LOCUS       XP_077504411             570 aa            linear   INV 25-NOV-2025
DEFINITION  tyrosine kinase receptor Cad96Ca [Amblyomma americanum].
ACCESSION   XP_077504411
VERSION     XP_077504411.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648285.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..570
                     /product="tyrosine kinase receptor Cad96Ca"
                     /calculated_mol_wt=63558
     Region          272..534
                     /region_name="PTKc"
                     /note="Catalytic domain of Protein Tyrosine Kinases;
                     cd00192"
                     /db_xref="CDD:270623"
     Site            order(273..277,281,299,301,348..351,354..355,407,411..412,
                     414,425,443..447,456,490)
                     /site_type="active"
                     /db_xref="CDD:270623"
     Site            order(273..274,276..277,281,299,301,348..351,354..355,
                     411..412,414,425)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270623"
     Site            order(407,411,443..447,456,490)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270623"
     Site            order(424..431,443..449)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270623"
     CDS             1..570
                     /gene="Cad96Ca"
                     /coded_by="XM_077648285.1:90..1802"
                     /db_xref="GeneID:144114499"
ORIGIN      
        1 mtvrtrdedh dvieygiepa vfldgssyfr inkrtgevfl srslagqags dyylfitand
       61 ghqtakievy vkvvkpssgl dgsdlleppp rpfpgggpgg spsghfappa pppslgpgkk
      121 rpnkeppppq pprppagptg tgqvrpdagv entadaeekg gahggtqgts ggidmtstvl
      181 pivavvgfap lvalffwlmh rrchrddklk pkkstdrlds nfphnfdlsp atlynprarr
      241 alsnryepdf lsslsehskw efprhhlrfi gilgegcfgq vwkceamnit gseepvivav
      301 ktlkenatek ekkellselg vmklinphpn vvtllgsctd rdplfvimey vpfgklqtyl
      361 resrversyg nlhgsskhlt srdltsfayq vakgmeylss kgiihrdlaa rnilvgenkt
      421 ckvadfgfar dvitthvyer ksegrlpirw mapeslydni fttktdvwsf gilmweivtl
      481 gstpypglaa aevmrrvrdg yrlekpehck remynimyyc wdpdpnerps fseltclldk
      541 llvsendyie ldrfpdhsyy nitnlsgekl
//
LOCUS       XP_077504412             198 aa            linear   INV 25-NOV-2025
DEFINITION  translocase of inner mitochondrial membrane 23 [Amblyomma
            americanum].
ACCESSION   XP_077504412
VERSION     XP_077504412.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648286.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..198
                     /product="translocase of inner mitochondrial membrane 23"
                     /calculated_mol_wt=20602
     Region          34..164
                     /region_name="TIM22"
                     /note="Mitochondrial import inner membrane translocase,
                     subunit TIM22 [Posttranslational modification, protein
                     turnover, chaperones]; cl02381"
                     /db_xref="CDD:470560"
     CDS             1..198
                     /gene="Tim23"
                     /coded_by="XM_077648286.1:132..728"
                     /db_xref="GeneID:144114500"
ORIGIN      
        1 mdrqsagyfg aaypaidpsf qipggqnapv spylnfdpay inaggpewif pegasrqrgr
       61 felafsqigg svmvgaafgg vtgfyrglre tslaghtgsv rrtqmlnfit kggagsanvl
      121 gviavmysgf gvllsfarga ddelntlaag tltgllykss sglrrclmgg gvglglaalc
      181 clwtskdrmk qmflthpa
//
LOCUS       XP_077504413             376 aa            linear   INV 25-NOV-2025
DEFINITION  actin-3-like [Amblyomma americanum].
ACCESSION   XP_077504413
VERSION     XP_077504413.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648287.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..376
                     /product="actin-3-like"
                     /calculated_mol_wt=41962
     Region          7..371
                     /region_name="ASKHA_NBD_actin"
                     /note="nucleotide-binding domain (NBD) of actin and
                     similar proteins; cd10224"
                     /db_xref="CDD:466823"
     CDS             1..376
                     /gene="LOC144114501"
                     /coded_by="XM_077648287.1:59..1189"
                     /db_xref="GeneID:144114501"
ORIGIN      
        1 mcedgeaalv fdngsgmfka gfagedaprt vfpsivgrpr hqgvivgmrq mdsyvgdqvq
       61 skrgiltlky piehgivtnw ddmekiwhht fynelrvape ehpvllteap lnpnairekm
      121 tqimfetfnt pamyvaiqav lslyasgrtt glvldcgdgv shtvpiydgy alphailrld
      181 lagrdltdyl mkiltergys ftttaeheiv rdikeklcyv aqdieqemvt aalssslekc
      241 yelpdgqvit vgnerfrcpe alfqpsflgm essgihetty isimkcdvdi rkglyantil
      301 sggttmypgi anrmqkeita lapstmkimi idppdrkysv wiggsilasl stfqqmwisk
      361 qdydeygpvi vhrkcf
//
LOCUS       XP_077504414             230 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114502 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504414
VERSION     XP_077504414.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648288.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..230
                     /product="uncharacterized protein LOC144114502 isoform X1"
                     /calculated_mol_wt=24473
     CDS             1..230
                     /gene="LOC144114502"
                     /coded_by="XM_077648288.1:90..782"
                     /db_xref="GeneID:144114502"
ORIGIN      
        1 miavtcllaa svlaarcsls haggahglad avyeannyvd tvlgvvlpqq vsranltsap
       61 lpgftvgnes davtvridgg vlfglgkqwq rrgdcratqw rgenitttch atldgmilim
      121 ygvaenndaa fienvtliml vknssariyv tekkqghtvh lrlgleslpl agangqnvrd
      181 ipspsrtlaa fprkqvslaa sqvlrgfien earqgvlrha fslihlplpl
//
LOCUS       XP_077504415             221 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114502 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504415
VERSION     XP_077504415.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648289.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..221
                     /product="uncharacterized protein LOC144114502 isoform X2"
                     /calculated_mol_wt=23576
     CDS             1..221
                     /gene="LOC144114502"
                     /coded_by="XM_077648289.1:123..788"
                     /db_xref="GeneID:144114502"
ORIGIN      
        1 miavtcllaa svlggahgla davyeannyv dtvlgvvlpq qvsranltsa plpgftvgne
       61 sdavtvridg gvlfglgkqw qrrgdcratq wrgenitttc hatldgmili mygvaennda
      121 afienvtlim lvknssariy vtekkqghtv hlrlgleslp lagangqnvr dipspsrtla
      181 afprkqvsla asqvlrgfie nearqgvlrh afslihlplp l
//
LOCUS       XP_077504416             393 aa            linear   INV 25-NOV-2025
DEFINITION  G-protein coupled receptor dmsr-1-like [Amblyomma americanum].
ACCESSION   XP_077504416
VERSION     XP_077504416.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648290.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..393
                     /product="G-protein coupled receptor dmsr-1-like"
                     /calculated_mol_wt=43286
     Region          64..364
                     /region_name="7tmA_FMRFamide_R-like"
                     /note="FMRFamide (Phe-Met-Arg-Phe) receptors and related
                     proteins, member of the class A family of
                     seven-transmembrane G protein-coupled receptors; cd14978"
                     /db_xref="CDD:410630"
     Region          64..90
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410630"
     Region          96..121
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410630"
     Site            order(118,121..122,138..143,145..146,149,194,196..200,233,
                     236..238,240..242,244..245,308,311..312,314..315,318,
                     331..332,334..336,339,342..343)
                     /site_type="other"
                     /note="putative ligand binding pocket [chemical binding]"
                     /db_xref="CDD:410630"
     Region          138..168
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410630"
     Region          180..200
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410630"
     Region          233..258
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410630"
     Region          289..319
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410630"
     Region          332..357
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410630"
     CDS             1..393
                     /gene="LOC144114504"
                     /coded_by="XM_077648290.1:479..1660"
                     /db_xref="GeneID:144114504"
ORIGIN      
        1 maeilnvsgm lqiadslyld ednlsltean lsgpfagfdi feeeelpyhg aelgrfhawy
       61 tshlhgylsm tvcvfgilan vlniivltqr nmvsptngil tglavadmlv iatylpytit
      121 thvmppartq sfgtavftlv hahvsvvfht vstwltvtla vwrflavsfp asskewcsmq
      181 rarwavvsvy vscalcclpv ylsftvhqtg tpqepsykvd fsnitranga llqnlnfwty
      241 svlmklvpcv altglslgll rvlyeakark rrlrpvasgh tghgggseea rdrttrmlla
      301 vlllflvtef psgiaallsg ilgddfflhv ymnfgevmdi lalvnsavnf ilycsmsrqf
      361 rkafaalftp rivakwfplt seppnstyat tcv
//
LOCUS       XP_077504417             393 aa            linear   INV 25-NOV-2025
DEFINITION  G-protein coupled receptor dmsr-1-like [Amblyomma americanum].
ACCESSION   XP_077504417
VERSION     XP_077504417.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648291.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..393
                     /product="G-protein coupled receptor dmsr-1-like"
                     /calculated_mol_wt=43286
     Region          64..364
                     /region_name="7tmA_FMRFamide_R-like"
                     /note="FMRFamide (Phe-Met-Arg-Phe) receptors and related
                     proteins, member of the class A family of
                     seven-transmembrane G protein-coupled receptors; cd14978"
                     /db_xref="CDD:410630"
     Region          64..90
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410630"
     Region          96..121
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410630"
     Site            order(118,121..122,138..143,145..146,149,194,196..200,233,
                     236..238,240..242,244..245,308,311..312,314..315,318,
                     331..332,334..336,339,342..343)
                     /site_type="other"
                     /note="putative ligand binding pocket [chemical binding]"
                     /db_xref="CDD:410630"
     Region          138..168
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410630"
     Region          180..200
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410630"
     Region          233..258
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410630"
     Region          289..319
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410630"
     Region          332..357
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410630"
     CDS             1..393
                     /gene="LOC144114504"
                     /coded_by="XM_077648291.1:474..1655"
                     /db_xref="GeneID:144114504"
ORIGIN      
        1 maeilnvsgm lqiadslyld ednlsltean lsgpfagfdi feeeelpyhg aelgrfhawy
       61 tshlhgylsm tvcvfgilan vlniivltqr nmvsptngil tglavadmlv iatylpytit
      121 thvmppartq sfgtavftlv hahvsvvfht vstwltvtla vwrflavsfp asskewcsmq
      181 rarwavvsvy vscalcclpv ylsftvhqtg tpqepsykvd fsnitranga llqnlnfwty
      241 svlmklvpcv altglslgll rvlyeakark rrlrpvasgh tghgggseea rdrttrmlla
      301 vlllflvtef psgiaallsg ilgddfflhv ymnfgevmdi lalvnsavnf ilycsmsrqf
      361 rkafaalftp rivakwfplt seppnstyat tcv
//
LOCUS       XP_077504418             393 aa            linear   INV 25-NOV-2025
DEFINITION  G-protein coupled receptor dmsr-1-like [Amblyomma americanum].
ACCESSION   XP_077504418
VERSION     XP_077504418.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648292.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..393
                     /product="G-protein coupled receptor dmsr-1-like"
                     /calculated_mol_wt=43286
     Region          64..364
                     /region_name="7tmA_FMRFamide_R-like"
                     /note="FMRFamide (Phe-Met-Arg-Phe) receptors and related
                     proteins, member of the class A family of
                     seven-transmembrane G protein-coupled receptors; cd14978"
                     /db_xref="CDD:410630"
     Region          64..90
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410630"
     Region          96..121
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410630"
     Site            order(118,121..122,138..143,145..146,149,194,196..200,233,
                     236..238,240..242,244..245,308,311..312,314..315,318,
                     331..332,334..336,339,342..343)
                     /site_type="other"
                     /note="putative ligand binding pocket [chemical binding]"
                     /db_xref="CDD:410630"
     Region          138..168
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410630"
     Region          180..200
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410630"
     Region          233..258
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410630"
     Region          289..319
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410630"
     Region          332..357
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410630"
     CDS             1..393
                     /gene="LOC144114504"
                     /coded_by="XM_077648292.1:300..1481"
                     /db_xref="GeneID:144114504"
ORIGIN      
        1 maeilnvsgm lqiadslyld ednlsltean lsgpfagfdi feeeelpyhg aelgrfhawy
       61 tshlhgylsm tvcvfgilan vlniivltqr nmvsptngil tglavadmlv iatylpytit
      121 thvmppartq sfgtavftlv hahvsvvfht vstwltvtla vwrflavsfp asskewcsmq
      181 rarwavvsvy vscalcclpv ylsftvhqtg tpqepsykvd fsnitranga llqnlnfwty
      241 svlmklvpcv altglslgll rvlyeakark rrlrpvasgh tghgggseea rdrttrmlla
      301 vlllflvtef psgiaallsg ilgddfflhv ymnfgevmdi lalvnsavnf ilycsmsrqf
      361 rkafaalftp rivakwfplt seppnstyat tcv
//
LOCUS       XP_077504419             393 aa            linear   INV 25-NOV-2025
DEFINITION  G-protein coupled receptor dmsr-1-like [Amblyomma americanum].
ACCESSION   XP_077504419
VERSION     XP_077504419.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648293.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..393
                     /product="G-protein coupled receptor dmsr-1-like"
                     /calculated_mol_wt=43286
     Region          64..364
                     /region_name="7tmA_FMRFamide_R-like"
                     /note="FMRFamide (Phe-Met-Arg-Phe) receptors and related
                     proteins, member of the class A family of
                     seven-transmembrane G protein-coupled receptors; cd14978"
                     /db_xref="CDD:410630"
     Region          64..90
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410630"
     Region          96..121
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410630"
     Site            order(118,121..122,138..143,145..146,149,194,196..200,233,
                     236..238,240..242,244..245,308,311..312,314..315,318,
                     331..332,334..336,339,342..343)
                     /site_type="other"
                     /note="putative ligand binding pocket [chemical binding]"
                     /db_xref="CDD:410630"
     Region          138..168
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410630"
     Region          180..200
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410630"
     Region          233..258
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410630"
     Region          289..319
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410630"
     Region          332..357
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410630"
     CDS             1..393
                     /gene="LOC144114504"
                     /coded_by="XM_077648293.1:326..1507"
                     /db_xref="GeneID:144114504"
ORIGIN      
        1 maeilnvsgm lqiadslyld ednlsltean lsgpfagfdi feeeelpyhg aelgrfhawy
       61 tshlhgylsm tvcvfgilan vlniivltqr nmvsptngil tglavadmlv iatylpytit
      121 thvmppartq sfgtavftlv hahvsvvfht vstwltvtla vwrflavsfp asskewcsmq
      181 rarwavvsvy vscalcclpv ylsftvhqtg tpqepsykvd fsnitranga llqnlnfwty
      241 svlmklvpcv altglslgll rvlyeakark rrlrpvasgh tghgggseea rdrttrmlla
      301 vlllflvtef psgiaallsg ilgddfflhv ymnfgevmdi lalvnsavnf ilycsmsrqf
      361 rkafaalftp rivakwfplt seppnstyat tcv
//
LOCUS       XP_077504421             562 aa            linear   INV 25-NOV-2025
DEFINITION  ornithine decarboxylase-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504421
VERSION     XP_077504421.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648295.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..562
                     /product="ornithine decarboxylase-like isoform X1"
                     /calculated_mol_wt=61598
     Region          157..520
                     /region_name="PLPDE_III_ODC"
                     /note="Type III Pyridoxal 5-phosphate (PLP)-Dependent
                     Enzyme Ornithine Decarboxylase; cd00622"
                     /db_xref="CDD:143482"
     Site            order(158,189,210..211,213..214,236,241,254,257,261,
                     286..288,409,411..412,427,429,431,434..435,450,469,471,
                     473,476,505,507,509..511,513)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143482"
     Site            order(187,189,208,272,315,318,354..355,392..395,472,501)
                     /site_type="active"
                     /db_xref="CDD:143482"
     Site            order(187,189,208,272,315,318,354..355,392..395,472,501)
                     /site_type="other"
                     /note="pyridoxal 5'-phosphate (PLP) binding site [chemical
                     binding]"
                     /db_xref="CDD:143482"
     Site            order(189,472)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:143482"
     Site            order(450,472..473,501,509)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:143482"
     CDS             1..562
                     /gene="LOC144114505"
                     /coded_by="XM_077648295.1:16..1704"
                     /db_xref="GeneID:144114505"
ORIGIN      
        1 mvggeekrek tttritstna kaikgrgrar hgrknreaea psgqghfgwk ratpatalvs
       61 srstashrel paapepvpgs qrrtllpatt rgvssavath pggapsannt gitstgawag
      121 slydavsdas rstsnaistp atgpitrlir teptiaddaf fvcdlrdlea kiklwrqemp
      181 rvtpfyavka csdpvilhil nfygvnfdcs nkaemmsvfd igvsperivy antvkctshl
      241 rfaqqrgvtl itfdsveeld kiedpnarll lriaaneygv qqsmnakygi tfrdaskvla
      301 aardmelnvv gvsfhvgcay khpeifalti edaravfdig admgfemtvl digggfpggv
      361 rkrehfnkvc eavrtsldkh fpassgveii gepgqffvts aytlvtkvvg kktkellidg
      421 ivhshenvyi neskynsipr dlypfmdisy rpleapydrp qnvlttlwga tcnpmdcvls
      481 kvpffdvcld ewllvdnmga ytlvlccgfn gfpmpevhyi atariaphvr rtidamvvra
      541 gyghlqqavk whrmrnglsh ka
//
LOCUS       XP_077504422             468 aa            linear   INV 25-NOV-2025
DEFINITION  ornithine decarboxylase-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504422
VERSION     XP_077504422.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648296.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..468
                     /product="ornithine decarboxylase-like isoform X2"
                     /calculated_mol_wt=51036
     Region          157..>450
                     /region_name="PLPDE_III"
                     /note="Type III Pyridoxal 5-phosphate (PLP)-Dependent
                     Enzymes; cl00261"
                     /db_xref="CDD:469695"
     Site            order(187,189,231,272,313,315,392..395)
                     /site_type="other"
                     /note="pyridoxal 5'-phosphate (PLP) binding site [chemical
                     binding]"
                     /db_xref="CDD:143484"
     Site            189
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:143484"
     CDS             1..468
                     /gene="LOC144114505"
                     /coded_by="XM_077648296.1:16..1422"
                     /db_xref="GeneID:144114505"
ORIGIN      
        1 mvggeekrek tttritstna kaikgrgrar hgrknreaea psgqghfgwk ratpatalvs
       61 srstashrel paapepvpgs qrrtllpatt rgvssavath pggapsannt gitstgawag
      121 slydavsdas rstsnaistp atgpitrlir teptiaddaf fvcdlrdlea kiklwrqemp
      181 rvtpfyavka csdpvilhil nfygvnfdcs nkaemmsvfd igvsperivy antvkctshl
      241 rfaqqrgvtl itfdsveeld kiedpnarll lriaaneygv qqsmnakygi tfrdaskvla
      301 aardmelnvv gvsfhvgcay khpeifalti edaravfdig admgfemtvl digggfpggv
      361 rkrehfnkvc eavrtsldkh fpassgveii gepgqffvts aytlvtkvvg kktkellidg
      421 ivhshenvyi neskynsipr dlypfmdisy rplegariyc kiegrphq
//
LOCUS       XP_077504423             436 aa            linear   INV 25-NOV-2025
DEFINITION  ornithine decarboxylase-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077504423
VERSION     XP_077504423.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648297.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..436
                     /product="ornithine decarboxylase-like isoform X3"
                     /calculated_mol_wt=48818
     Region          31..394
                     /region_name="PLPDE_III_ODC"
                     /note="Type III Pyridoxal 5-phosphate (PLP)-Dependent
                     Enzyme Ornithine Decarboxylase; cd00622"
                     /db_xref="CDD:143482"
     Site            order(32,63,84..85,87..88,110,115,128,131,135,160..162,
                     283,285..286,301,303,305,308..309,324,343,345,347,350,379,
                     381,383..385,387)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143482"
     Site            order(61,63,82,146,189,192,228..229,266..269,346,375)
                     /site_type="active"
                     /db_xref="CDD:143482"
     Site            order(61,63,82,146,189,192,228..229,266..269,346,375)
                     /site_type="other"
                     /note="pyridoxal 5'-phosphate (PLP) binding site [chemical
                     binding]"
                     /db_xref="CDD:143482"
     Site            order(63,346)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:143482"
     Site            order(324,346..347,375,383)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:143482"
     CDS             1..436
                     /gene="LOC144114505"
                     /coded_by="XM_077648297.1:60..1370"
                     /db_xref="GeneID:144114505"
ORIGIN      
        1 mgdnkdsedl vhveekvtdv arkvidsqia ddaffvcdlr dleakiklwr qemprvtpfy
       61 avkacsdpvi lhilnfygvn fdcsnkaemm svfdigvspe rivyantvkc tshlrfaqqr
      121 gvtlitfdsv eeldkiedpn arlllriaan eygvqqsmna kygitfrdas kvlaaardme
      181 lnvvgvsfhv gcaykhpeif altiedarav fdigadmgfe mtvldigggf pggvrkrehf
      241 nkvceavrts ldkhfpassg veiigepgqf fvtsaytlvt kvvgkktkel lidgivhshe
      301 nvyineskyn siprdlypfm disyrpleap ydrpqnvltt lwgatcnpmd cvlskvpffd
      361 vcldewllvd nmgaytlvlc cgfngfpmpe vhyiataria phvrrtidam vvragyghlq
      421 qavkwhrmrn glshka
//
LOCUS       XP_077504424             423 aa            linear   INV 25-NOV-2025
DEFINITION  ornithine decarboxylase-like [Amblyomma americanum].
ACCESSION   XP_077504424
VERSION     XP_077504424.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648298.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..423
                     /product="ornithine decarboxylase-like"
                     /calculated_mol_wt=46551
     Region          32..393
                     /region_name="PLPDE_III"
                     /note="Type III Pyridoxal 5-phosphate (PLP)-Dependent
                     Enzymes; cl00261"
                     /db_xref="CDD:469695"
     Site            order(62,64,106,147,188,190,266..269)
                     /site_type="other"
                     /note="pyridoxal 5'-phosphate (PLP) binding site [chemical
                     binding]"
                     /db_xref="CDD:143484"
     Site            64
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:143484"
     CDS             1..423
                     /gene="LOC144114506"
                     /coded_by="XM_077648298.1:109..1380"
                     /db_xref="GeneID:144114506"
ORIGIN      
        1 mygeengfss vfieedissv aqkvidrkvy eeeaffvcdv rdiqsnislw qqqlpcvkpy
       61 ynvsccsdlv vlrtlsargv nfacgskhem qillesgvgp dsmlynsttk cgshlkyaee
      121 rglrlmcfds aaelarindt narlllrlam hddscqsfln dqfgcsleea tqllrlaktt
      181 hrivvgvsfh mgeisvhdea fvrtvqkara vfdlglqagf pmtildiggg fpiasrkeef
      241 vevcrlvrla lgtyfpsssg ttliaepgrf fvasaftlvt kvvgkrkkga vangecedye
      301 evfineskyn siprdashlt gmslrplvnv ekprsvltaf waatsnprdl ifdtqlfwpl
      361 qvndwllmdn vgayslvlac afngsgtppv vyvasandad ivkkaltaas vcsgytqler
      421 aik
//
LOCUS       XP_077504425             641 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114507 [Amblyomma americanum].
ACCESSION   XP_077504425
VERSION     XP_077504425.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648299.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..641
                     /product="uncharacterized protein LOC144114507"
                     /calculated_mol_wt=70615
     CDS             1..641
                     /gene="LOC144114507"
                     /coded_by="XM_077648299.1:489..2414"
                     /db_xref="GeneID:144114507"
ORIGIN      
        1 mplnkfrsrh kfgkkksarg dnfrkrpasp ecsenvqepv asevpadeig ltssatsths
       61 grvrcdttml qpsdllenkr kaegilqele staatkkkld figscddwpl dgtdgrradd
      121 safsivslds fnellravkc rvcggdvhvs kgnreyglav klslvcvncg dtasawsspr
      181 aegsqkinpf avnilaaram qstgnrrtal ndvfaamnis hrglhtktwq syvkskltpa
      241 atraaeevlk dsansvrqly relnldnpgn isvsfdgswm trghssrigv gavielftgl
      301 vldyvvfsnf cagcergpkd ddpsyqawsd shlcqkntdk ksgemevqag lvlferswek
      361 hglryttvls dgdsrtflal qessvygyik iqkedctnhv qkrmgtalrn aiakhkgnak
      421 eslsgkgklt gdlvtkltsy yswalkshag digamhkavm atyyhvtsnd saanhtlcpt
      481 gpnswcrqna aaakgepapk hrynlpphvc kalfpiyerl gdpkllqrcl rgktqnnnes
      541 lhsliwslap kerhaslfsv qaavaeavvr fnagnlkasf qilselsmnp gqhnirrmte
      601 kdrrrtaesa rkrastetmh qalkkrhssq tkqsdympga y
//
LOCUS       XP_077504426             609 aa            linear   INV 25-NOV-2025
DEFINITION  negative elongation factor B-like [Amblyomma americanum].
ACCESSION   XP_077504426
VERSION     XP_077504426.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648300.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..609
                     /product="negative elongation factor B-like"
                     /calculated_mol_wt=67884
     Region          105..459
                     /region_name="COBRA1"
                     /note="Cofactor of BRCA1 (COBRA1); pfam06209"
                     /db_xref="CDD:461851"
     CDS             1..609
                     /gene="LOC144114508"
                     /coded_by="XM_077648300.1:404..2233"
                     /db_xref="GeneID:144114508"
ORIGIN      
        1 magleevgip greflrealt ncsdplraie efqeengill pslrpmlpll dlhgvcrlef
       61 htsvleelke rllqqidglg kqegrerdkk lkemlqksfp vikvpalrpv vmrimknmdh
      121 vdekylkqlv snktlykecd vqvkrqiwqe hqslfgdevl pllsqyirek dealwsltpp
      181 vaagataaea ssffgsspkq rrqgailqrl lvmvgrnvvl ydmvlqfvrt lflrtrnvhy
      241 ctlrvellma lhdseiqdit avdpchkftw cldacirekn idtkrsrelq gfldsikrgq
      301 eqvlgdlsmt lcdpyainfl aqsalkiinh linveglprd nqvlvlvlrm lalglqawem
      361 istqqykepk ldvqlvtkfl palmslmvdd qvrslnsrlp qddresaitt iehsgpppda
      421 yqayvqenav tsvlalyytf htarqrdrtg vmrvlgslag aegqrayqdp flhtlvghla
      481 lmaddfaqed fctvvfdeff lsglsgsasg sglsgsasgs gtlaalpste gasnaarhll
      541 rllwhvhakl ppsrlqglmk tlqplvqane glkpavqalq dkvkdltppa aepeppdspl
      601 lsvpvpapv
//
LOCUS       XP_077504427             609 aa            linear   INV 25-NOV-2025
DEFINITION  negative elongation factor B-like [Amblyomma americanum].
ACCESSION   XP_077504427
VERSION     XP_077504427.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648301.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..609
                     /product="negative elongation factor B-like"
                     /calculated_mol_wt=67884
     Region          105..459
                     /region_name="COBRA1"
                     /note="Cofactor of BRCA1 (COBRA1); pfam06209"
                     /db_xref="CDD:461851"
     CDS             1..609
                     /gene="LOC144114508"
                     /coded_by="XM_077648301.1:410..2239"
                     /db_xref="GeneID:144114508"
ORIGIN      
        1 magleevgip greflrealt ncsdplraie efqeengill pslrpmlpll dlhgvcrlef
       61 htsvleelke rllqqidglg kqegrerdkk lkemlqksfp vikvpalrpv vmrimknmdh
      121 vdekylkqlv snktlykecd vqvkrqiwqe hqslfgdevl pllsqyirek dealwsltpp
      181 vaagataaea ssffgsspkq rrqgailqrl lvmvgrnvvl ydmvlqfvrt lflrtrnvhy
      241 ctlrvellma lhdseiqdit avdpchkftw cldacirekn idtkrsrelq gfldsikrgq
      301 eqvlgdlsmt lcdpyainfl aqsalkiinh linveglprd nqvlvlvlrm lalglqawem
      361 istqqykepk ldvqlvtkfl palmslmvdd qvrslnsrlp qddresaitt iehsgpppda
      421 yqayvqenav tsvlalyytf htarqrdrtg vmrvlgslag aegqrayqdp flhtlvghla
      481 lmaddfaqed fctvvfdeff lsglsgsasg sglsgsasgs gtlaalpste gasnaarhll
      541 rllwhvhakl ppsrlqglmk tlqplvqane glkpavqalq dkvkdltppa aepeppdspl
      601 lsvpvpapv
//
LOCUS       XP_077504428            1145 aa            linear   INV 25-NOV-2025
DEFINITION  calcium-activated chloride channel regulator 4A-like [Amblyomma
            americanum].
ACCESSION   XP_077504428
VERSION     XP_077504428.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648302.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1145
                     /product="calcium-activated chloride channel regulator
                     4A-like"
                     /calculated_mol_wt=125201
     Region          32..841
                     /region_name="hCaCC"
                     /note="calcium-activated chloride channel protein 1;
                     TIGR00868"
                     /db_xref="CDD:129946"
     CDS             1..1145
                     /gene="LOC144114509"
                     /coded_by="XM_077648302.1:2361..5798"
                     /db_xref="GeneID:144114509"
ORIGIN      
        1 mksifavgva afaalclprw tavgaakpqp phlegngytd ivvrlphgla prdpgelfgq
       61 lerllrrasg kllqasdgqw yfksirvlvp pemntpknll lqdatwelfh dahivltksg
      121 skckarqsrg cgergdlval plsaldssad idtaassvvr swaqfrygvf eetafgskpq
      181 wcfydgsfwr ptgcyttnvt arpqkstdgf tecskkdvsv kqifqsasep asslmftssi
      241 pkvqkfcdar gnhshlrwap tkhntlckar svwevirdsp dyspsaprls fqpatlfdyv
      301 kprthryfyv vqacapdltt qtytkimast neglrrflys aprasrwnlv lldgsnvtap
      361 aepdfdsnnt twqarweylr eldrnikytc vepgvestfr iilkvaqnea ttvvallhsg
      421 svteaaidal aaadtvrlvl ilfdtgappg stwrsaalrd sqlfmvptaa sqetmaalvd
      481 lalhtthhat adlhgdvvkv fqrtdvllqd gvkgtfhfks fgsnvlsfiv acpdvysiee
      541 sdvtfngtvv psyrvkmsmf atelqlsedp divsvpyalt arkssqrcsf masirgsvqe
      601 prvrlrgwfr rnkvdvmftv pqviyaelly gerpvkgarv eavvyhvaqn avattvvqlr
      661 dngllepdvs kgdgvysgyf laasqqggwy svvvtatgsq agsngsvaas fqraefvgsf
      721 wssayagqgg vhfppgtvrd lsvirvdlpn vtlewtapgs qydsgdgags syelrysqdr
      781 rllsenfanq pgleleqqkq pgdlewatvq lpedkdenta vyffalrtkn mehrysdisn
      841 vvrvaigvls ststdenvsa ngtsistnet stdsvmggsn sttgpllett rdgnesssvl
      901 lyfnrfteeq fyyvvagvsg gfvllvllin cllclmclrc rksknkrrnt dcvvienavg
      961 sdkpinayde qskfdapgnv galrqklsnl sqrrdganna ttgdsgtrsk dspdagskav
     1021 qpsvkasdre apialeslke rapssgvpsa mprsiardkp rsirtlvses rannsgekvt
     1081 assgpepllq rrpsgslrss krsnasssrt trhsdrsrrt eggrtsgfgd htrkrsgdyy
     1141 dlgvv
//
LOCUS       XP_077504429             204 aa            linear   INV 25-NOV-2025
DEFINITION  protein spaetzle 3-like [Amblyomma americanum].
ACCESSION   XP_077504429
VERSION     XP_077504429.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648303.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..204
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..204
                     /product="protein spaetzle 3-like"
                     /calculated_mol_wt=23260
     Region          109..196
                     /region_name="Spaetzle"
                     /note="pfam16077"
                     /db_xref="CDD:465007"
     CDS             1..204
                     /gene="LOC144114510"
                     /coded_by="XM_077648303.1:244..858"
                     /db_xref="GeneID:144114510"
ORIGIN      
        1 mdwrqltlal llalcaldlk aeilkgypci rrlnqlycpt pgnsypkeri dmfidenkal
       61 irrmfgeyss pdedgghrdh yetydgktih trtersrssf agassnkvda cesaveivtp
      121 ywasnsagki raivntqhlq qaiqqevcqs vqtkkcnndc sceqkykwhr llaydpdddc
      181 kgifmdwflf psccvcrcan pikk
//
LOCUS       XP_077504430             726 aa            linear   INV 25-NOV-2025
DEFINITION  atrial natriuretic peptide-converting enzyme-like [Amblyomma
            americanum].
ACCESSION   XP_077504430
VERSION     XP_077504430.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648304.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 12% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..726
                     /product="atrial natriuretic peptide-converting
                     enzyme-like"
                     /calculated_mol_wt=78778
     Region          168..284
                     /region_name="CRD_FZ"
                     /note="CRD_domain cysteine-rich domain, also known as Fz
                     (frizzled) domain; cd07066"
                     /db_xref="CDD:143549"
     Site            order(176,178,180,183..185)
                     /site_type="other"
                     /note="putative Wnt binding site [polypeptide binding]"
                     /db_xref="CDD:143549"
     Region          <296..314
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(297,300,304,310..311)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            307..311
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          317..351
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(322,330,341..342)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(334,337,341,347..348)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            344..348
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          531..699
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cl21584"
                     /db_xref="CDD:473915"
     CDS             1..726
                     /gene="LOC144114511"
                     /coded_by="XM_077648304.1:1..2181"
                     /db_xref="GeneID:144114511"
ORIGIN      
        1 mdvrkmfnis aratyyervy rgaftvlegd kfseslsnsl addfvrkada yrakldrlfn
       61 ssaafrgtev iafqrredre ltvhfnvhfs pygpvdagel ylamadellr grlgvfqglk
      121 vdpeslslqe rrataslpes wtprrrypgy laglstsrpa vgrqaelrce pltldhcras
      181 lpynstaypn avghatardv yrdlvayrql adsechplaa elvcrllqpq cddaerrvvl
      241 pcrefcqefw sscqqqmpla iaghidcgcl peysaagsch tkpgcanelk arskeshicd
      301 gvvdcpdfsd etscdncgpg llhcgdrqcv diaqrcdarl dcdngadeqn clslpelggy
      361 lrayqrghfr kvcvdglpeg rrrdsllkql adtacaalnl gppsrvelhh dgnssdsylq
      421 lldppglrfs spgacrsdlv vyllcsppgv pnwaeaddsa rgrtatlleg cvvlslilag
      481 rsggkrptte egarqpsssi ffsgntaplg giaaaaghrs rtwvcvhtht hevrhgdwpw
      541 hvmllqagrh vcdgtlvadr wvltagaclp qgrdpsgewq arlgsvrkes aspfdvraai
      601 aavlrsadgv lallrldaav pqsravrpac lprtpppqag rcatlaygvk gdqleqvnle
      661 asecgpavlc grqascvhea lagrqlmcwh gdrwqlagva aepcgggaqr fhglgphlaw
      721 aeaals
//
LOCUS       XP_077504431             654 aa            linear   INV 25-NOV-2025
DEFINITION  RUN and FYVE domain-containing protein 2 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504431
VERSION     XP_077504431.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648305.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..654
                     /product="RUN and FYVE domain-containing protein 2 isoform
                     X1"
                     /calculated_mol_wt=74273
     Region          80..235
                     /region_name="RUN_RUFY1_like"
                     /note="RUN domain found in RUN and FYVE domain-containing
                     proteins, RUFY1, RUFY2, RUFY3 and similar proteins;
                     cd17681"
                     /db_xref="CDD:439043"
     Region          <319..>585
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          586..643
                     /region_name="FYVE_RUFY1_like"
                     /note="FYVE domain found in RUN and FYVE domain-containing
                     protein RUFY1, RUFY2 and similar proteins; cd15721"
                     /db_xref="CDD:277261"
     Site            order(586,589,607..612,614..615,637..639)
                     /site_type="other"
                     /note="putative phosphatidylinositol 3-phosphate binding
                     site [chemical binding]"
                     /db_xref="CDD:277261"
     CDS             1..654
                     /gene="LOC144114513"
                     /coded_by="XM_077648305.1:113..2077"
                     /db_xref="GeneID:144114513"
ORIGIN      
        1 mptsqsaadt rsplgedhpe saelsrpmak dtiylcnfrv svdgewlclk eladvqfdtq
       61 kplavttpyt fedrdpvaie rsnllnltkl vvkeliessl kfgrmldsdh pplqhffill
      121 ehvlrhglkp kkgilgpkke lwnvleavek fvpeaaditt svrdlptvks qlgrarawlr
      181 lalmqkklad yfrlvidkkd ellrdfyedd alilseeavv iggllvglnv idcnlcvkee
      241 dldsqqgvid fglylrenah tdsseegvep asmtavldqk nyieelnrhl natvtnlqqk
      301 veqlqttnal mkedlaiskn qilsleeenr vlrehqdsiv edhqrklqna kadmcaeret
      361 lqanqaglds lytevrrqls eevdrrqeae malkllekdi hekqdsivsl rrqledikai
      421 nlqmynklqd cesslkakte qaakleqkva qltsslkeae aktqsvekqk qafeettrsl
      481 gqqlidkdqk rsllesdlki erewrvslqa tvaeqqeeam klqrgidayq qvaqdherlk
      541 vefselerkc aeheltleel gcqlsesklq vadlkennls lkeavwtsdk dasccrqcsk
      601 pfsvarrkhh crscgeifcn acsdntmplp ssakpvrvcd acqtvllqry sats
//
LOCUS       XP_077504432             654 aa            linear   INV 25-NOV-2025
DEFINITION  RUN and FYVE domain-containing protein 2 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504432
VERSION     XP_077504432.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648306.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..654
                     /product="RUN and FYVE domain-containing protein 2 isoform
                     X2"
                     /calculated_mol_wt=74245
     Region          80..235
                     /region_name="RUN_RUFY1_like"
                     /note="RUN domain found in RUN and FYVE domain-containing
                     proteins, RUFY1, RUFY2, RUFY3 and similar proteins;
                     cd17681"
                     /db_xref="CDD:439043"
     Region          <283..561
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          586..643
                     /region_name="FYVE_RUFY1_like"
                     /note="FYVE domain found in RUN and FYVE domain-containing
                     protein RUFY1, RUFY2 and similar proteins; cd15721"
                     /db_xref="CDD:277261"
     Site            order(586,589,607..612,614..615,637..639)
                     /site_type="other"
                     /note="putative phosphatidylinositol 3-phosphate binding
                     site [chemical binding]"
                     /db_xref="CDD:277261"
     CDS             1..654
                     /gene="LOC144114513"
                     /coded_by="XM_077648306.1:113..2077"
                     /db_xref="GeneID:144114513"
ORIGIN      
        1 mptsqsaadt rsplgedhpe saelsrpmak dtiylcnfrv svdgewlclk eladvqfdtq
       61 kplavttpyt fedrdpvaie rsnllnltkl vvkeliessl kfgrmldsdh pplqhffill
      121 ehvlrhglkp kkgilgpkke lwnvleavek fvpeaaditt svrdlptvks qlgrarawlr
      181 lalmqkklad yfrlvidkkd ellrdfyedd alilseeavv iggllvglnv idcnlcvkee
      241 dldsqqgvid fglylrenah tdsseegvep asmtavldqk nyieelnrhl natvtnlqqk
      301 veqlqttnal mkedlaiskn qilsleeenr vlrehqdsiv edhqrklqna kadmcaeret
      361 lqanqaglds lytevrrqls eevdrrqate malkllekdi hekqdsivsl rrqledikai
      421 nlqmynklqd cesslkakte qaakleqkva qltsslkeae aktqsvekqk qafeettrsl
      481 gqqlidkdqk rsllesdlki erewrvslqa tvaeqqeeam klqrgidayq qvaqdherlk
      541 vefselerkc aeheltleel gcqlsesklq vadlkennls lkeavwtsdk dasccrqcsk
      601 pfsvarrkhh crscgeifcn acsdntmplp ssakpvrvcd acqtvllqry sats
//
LOCUS       XP_077504433             643 aa            linear   INV 25-NOV-2025
DEFINITION  RUN and FYVE domain-containing protein 2 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504433
VERSION     XP_077504433.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648307.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..643
                     /product="RUN and FYVE domain-containing protein 2 isoform
                     X3"
                     /calculated_mol_wt=72828
     Region          69..224
                     /region_name="RUN_RUFY1_like"
                     /note="RUN domain found in RUN and FYVE domain-containing
                     proteins, RUFY1, RUFY2, RUFY3 and similar proteins;
                     cd17681"
                     /db_xref="CDD:439043"
     Region          <308..>574
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          575..632
                     /region_name="FYVE_RUFY1_like"
                     /note="FYVE domain found in RUN and FYVE domain-containing
                     protein RUFY1, RUFY2 and similar proteins; cd15721"
                     /db_xref="CDD:277261"
     Site            order(575,578,596..601,603..604,626..628)
                     /site_type="other"
                     /note="putative phosphatidylinositol 3-phosphate binding
                     site [chemical binding]"
                     /db_xref="CDD:277261"
     CDS             1..643
                     /gene="LOC144114513"
                     /coded_by="XM_077648307.1:21..1952"
                     /db_xref="GeneID:144114513"
ORIGIN      
        1 maastvtsfv dtgektnvlk gdkstsatsa nstsnqasas erhpifllsa dysrrekyyr
       61 anrdpvaier snllnltklv vkeliesslk fgrmldsdhp plqhffille hvlrhglkpk
      121 kgilgpkkel wnvleavekf vpeaaditts vrdlptvksq lgrarawlrl almqkklady
      181 frlvidkkde llrdfyedda lilseeavvi ggllvglnvi dcnlcvkeed ldsqqgvidf
      241 glylrenaht dsseegvepa smtavldqkn yieelnrhln atvtnlqqkv eqlqttnalm
      301 kedlaisknq ilsleeenrv lrehqdsive dhqrklqnak admcaeretl qanqagldsl
      361 ytevrrqlse evdrrqeaem alkllekdih ekqdsivslr rqledikain lqmynklqdc
      421 esslkakteq aakleqkvaq ltsslkeaea ktqsvekqkq afeettrslg qqlidkdqkr
      481 sllesdlkie rewrvslqat vaeqqeeamk lqrgidayqq vaqdherlkv efselerkca
      541 eheltleelg cqlsesklqv adlkennlsl keavwtsdkd asccrqcskp fsvarrkhhc
      601 rscgeifcna csdntmplps sakpvrvcda cqtvllqrys ats
//
LOCUS       XP_077504434             640 aa            linear   INV 25-NOV-2025
DEFINITION  RUN and FYVE domain-containing protein 2 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077504434
VERSION     XP_077504434.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648308.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..640
                     /product="RUN and FYVE domain-containing protein 2 isoform
                     X4"
                     /calculated_mol_wt=72711
     Region          80..235
                     /region_name="RUN_RUFY1_like"
                     /note="RUN domain found in RUN and FYVE domain-containing
                     proteins, RUFY1, RUFY2, RUFY3 and similar proteins;
                     cd17681"
                     /db_xref="CDD:439043"
     Region          <296..>562
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          572..629
                     /region_name="FYVE_RUFY1_like"
                     /note="FYVE domain found in RUN and FYVE domain-containing
                     protein RUFY1, RUFY2 and similar proteins; cd15721"
                     /db_xref="CDD:277261"
     Site            order(572,575,593..598,600..601,623..625)
                     /site_type="other"
                     /note="putative phosphatidylinositol 3-phosphate binding
                     site [chemical binding]"
                     /db_xref="CDD:277261"
     CDS             1..640
                     /gene="LOC144114513"
                     /coded_by="XM_077648308.1:113..2035"
                     /db_xref="GeneID:144114513"
ORIGIN      
        1 mptsqsaadt rsplgedhpe saelsrpmak dtiylcnfrv svdgewlclk eladvqfdtq
       61 kplavttpyt fedrdpvaie rsnllnltkl vvkeliessl kfgrmldsdh pplqhffill
      121 ehvlrhglkp kkgilgpkke lwnvleavek fvpeaaditt svrdlptvks qlgrarawlr
      181 lalmqkklad yfrlvidkkd ellrdfyedd alilseeavv iggllvglnv idcnlcvkee
      241 dldsqqgvid fglylrenah tdsseegvep asmtavldqk nyieelnrhl natvtnlqqk
      301 veqlqttnal mkedlaiskn qilsleeenr vlrehqdsiv edhqrklqan qagldslyte
      361 vrrqlseevd rrqeaemalk llekdihekq dsivslrrql edikainlqm ynklqdcess
      421 lkakteqaak leqkvaqlts slkeaeaktq svekqkqafe ettrslgqql idkdqkrsll
      481 esdlkierew rvslqatvae qqeeamklqr gidayqqvaq dherlkvefs elerkcaehe
      541 ltleelgcql sesklqvadl kennlslkea vwtsdkdasc crqcskpfsv arrkhhcrsc
      601 geifcnacsd ntmplpssak pvrvcdacqt vllqrysats
//
LOCUS       XP_077504435             629 aa            linear   INV 25-NOV-2025
DEFINITION  RUN and FYVE domain-containing protein 2 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077504435
VERSION     XP_077504435.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648309.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..629
                     /product="RUN and FYVE domain-containing protein 2 isoform
                     X5"
                     /calculated_mol_wt=71266
     Region          69..224
                     /region_name="RUN_RUFY1_like"
                     /note="RUN domain found in RUN and FYVE domain-containing
                     proteins, RUFY1, RUFY2, RUFY3 and similar proteins;
                     cd17681"
                     /db_xref="CDD:439043"
     Region          <285..>551
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          561..618
                     /region_name="FYVE_RUFY1_like"
                     /note="FYVE domain found in RUN and FYVE domain-containing
                     protein RUFY1, RUFY2 and similar proteins; cd15721"
                     /db_xref="CDD:277261"
     Site            order(561,564,582..587,589..590,612..614)
                     /site_type="other"
                     /note="putative phosphatidylinositol 3-phosphate binding
                     site [chemical binding]"
                     /db_xref="CDD:277261"
     CDS             1..629
                     /gene="LOC144114513"
                     /coded_by="XM_077648309.1:22..1911"
                     /db_xref="GeneID:144114513"
ORIGIN      
        1 maastvtsfv dtgektnvlk gdkstsatsa nstsnqasas erhpifllsa dysrrekyyr
       61 anrdpvaier snllnltklv vkeliesslk fgrmldsdhp plqhffille hvlrhglkpk
      121 kgilgpkkel wnvleavekf vpeaaditts vrdlptvksq lgrarawlrl almqkklady
      181 frlvidkkde llrdfyedda lilseeavvi ggllvglnvi dcnlcvkeed ldsqqgvidf
      241 glylrenaht dsseegvepa smtavldqkn yieelnrhln atvtnlqqkv eqlqttnalm
      301 kedlaisknq ilsleeenrv lrehqdsive dhqrklqanq agldslytev rrqlseevdr
      361 rqeaemalkl lekdihekqd sivslrrqle dikainlqmy nklqdcessl kakteqaakl
      421 eqkvaqltss lkeaeaktqs vekqkqafee ttrslgqqli dkdqkrslle sdlkierewr
      481 vslqatvaeq qeeamklqrg idayqqvaqd herlkvefse lerkcaehel tleelgcqls
      541 esklqvadlk ennlslkeav wtsdkdascc rqcskpfsva rrkhhcrscg eifcnacsdn
      601 tmplpssakp vrvcdacqtv llqrysats
//
LOCUS       XP_077504436             450 aa            linear   INV 25-NOV-2025
DEFINITION  pancreatic lipase-related protein 2-like [Amblyomma americanum].
ACCESSION   XP_077504436
VERSION     XP_077504436.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648310.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..450
                     /product="pancreatic lipase-related protein 2-like"
                     /calculated_mol_wt=49555
     Region          154..435
                     /region_name="Pancreat_lipase_like"
                     /note="Pancreatic lipase-like enzymes. Lipases are
                     esterases that can hydrolyze long-chain acyl-triglycerides
                     into di- and monoglycerides, glycerol, and free fatty
                     acids at a water/lipid interface. A typical feature of
                     lipases is 'interfacial activation,' the...; cd00707"
                     /db_xref="CDD:238363"
     Site            274..278
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238363"
     Site            order(276,304,372)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238363"
     CDS             1..450
                     /gene="LOC144114514"
                     /coded_by="XM_077648310.1:16..1368"
                     /db_xref="GeneID:144114514"
ORIGIN      
        1 mpslfahaac vhtwsdkklp argppqlsta qshhwlpkvl llvmllkfcl vallaashae
       61 trdankshhv lkklaakgks dgccsdmdmi saeelkwgkk mlqsihlane avkklpktsf
      121 ysdqtvcydk vgcfdrlggk fshfvahprs pkvigtkfll fsrlnrkvpa hldyndnatl
      181 dashfnkakd lvfvihgfgs naekswvtsm rdaflrlkdv nvvlvdwrkg cvapdymaas
      241 anaalvgrqv slliqalilh hpktvrasnv hlvgfslgaq vagfsgrhfi natgtkigrl
      301 taldaagplf etyefqvske dasfvdaiht tagsnvlagl lgmespfgdv nfypnggksq
      361 pgcwffdlyc hhrravqyfi eslhenrhcl fmsnpcddid tflnskcsvt gpqgemgyfs
      421 tkasgrgsqf lwtnddnpfc kdsrhkatsh
//
LOCUS       XP_077504437             414 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114515 [Amblyomma americanum].
ACCESSION   XP_077504437
VERSION     XP_077504437.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648311.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..414
                     /product="uncharacterized protein LOC144114515"
                     /calculated_mol_wt=46187
     Region          29..151
                     /region_name="LYZ_C_invert"
                     /note="C-type invertebrate lysozyme; cd16899"
                     /db_xref="CDD:381618"
     Region          189..>331
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     CDS             1..414
                     /gene="LOC144114515"
                     /coded_by="XM_077648311.1:446..1690"
                     /db_xref="GeneID:144114515"
ORIGIN      
        1 merhrrrsag yaltglvlas vgcavawari ydrcelalel hdrfkfpkrd ldkwlclayw
       61 esrydtrayh kgkydgsgdh gifqindkhw cqphqghsen vcrmpcyllr ddnlyddiec
      121 vnkifrrhgf hawmmfshkc sgntleffng cdfrirrgdm tnsptglhit iepppilekl
      181 sqlskgiyqa gshpvptrra pdpstgpsag pppprwapki nvpqklrpva vrpprpqppp
      241 kketnallpq rqpppmkrvp appppkppri vpvaaarpsr pqgprfpala rpsltraplp
      301 pktvggllvp qesvghavfg vpppfaptlp gahhphpppp phhlvavppq glamfglrrg
      361 ppplpipihv ppppqqqqvv fkrtktrrlp gglrlrpflg farssrsfkn kgkt
//
LOCUS       XP_077504438             414 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114515 [Amblyomma americanum].
ACCESSION   XP_077504438
VERSION     XP_077504438.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648312.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..414
                     /product="uncharacterized protein LOC144114515"
                     /calculated_mol_wt=46187
     Region          29..151
                     /region_name="LYZ_C_invert"
                     /note="C-type invertebrate lysozyme; cd16899"
                     /db_xref="CDD:381618"
     Region          189..>331
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     CDS             1..414
                     /gene="LOC144114515"
                     /coded_by="XM_077648312.1:443..1687"
                     /db_xref="GeneID:144114515"
ORIGIN      
        1 merhrrrsag yaltglvlas vgcavawari ydrcelalel hdrfkfpkrd ldkwlclayw
       61 esrydtrayh kgkydgsgdh gifqindkhw cqphqghsen vcrmpcyllr ddnlyddiec
      121 vnkifrrhgf hawmmfshkc sgntleffng cdfrirrgdm tnsptglhit iepppilekl
      181 sqlskgiyqa gshpvptrra pdpstgpsag pppprwapki nvpqklrpva vrpprpqppp
      241 kketnallpq rqpppmkrvp appppkppri vpvaaarpsr pqgprfpala rpsltraplp
      301 pktvggllvp qesvghavfg vpppfaptlp gahhphpppp phhlvavppq glamfglrrg
      361 ppplpipihv ppppqqqqvv fkrtktrrlp gglrlrpflg farssrsfkn kgkt
//
LOCUS       XP_077504439             394 aa            linear   INV 25-NOV-2025
DEFINITION  pancreatic lipase-related protein 2-like [Amblyomma americanum].
ACCESSION   XP_077504439
VERSION     XP_077504439.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648313.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..394
                     /product="pancreatic lipase-related protein 2-like"
                     /calculated_mol_wt=44635
     Region          92..374
                     /region_name="Pancreat_lipase_like"
                     /note="Pancreatic lipase-like enzymes. Lipases are
                     esterases that can hydrolyze long-chain acyl-triglycerides
                     into di- and monoglycerides, glycerol, and free fatty
                     acids at a water/lipid interface. A typical feature of
                     lipases is 'interfacial activation,' the...; cd00707"
                     /db_xref="CDD:238363"
     Site            211..215
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238363"
     Site            order(213,241,310)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238363"
     CDS             1..394
                     /gene="LOC144114517"
                     /coded_by="XM_077648313.1:125..1309"
                     /db_xref="GeneID:144114517"
ORIGIN      
        1 mllrlprptv viilfavsvs mvqrtgglls mltnaadeed iknegkwnlk fplnafslik
       61 dtrcyrnvgc frkddkyrlt hplvlpkrpr yvrtkfyayc renmngrrlr krtmrrdaap
      121 ffsrpkdlvi lvhgytqnvn stwmhelkea llkekdcnvi ivdwgrgcrs pkyltavgnt
      181 alvgrqisll vqrlaklfrg avtadrvhlv gfslgaqvsg fcgrhfkket gkklarisal
      241 daarplfeqs dvyvsrkdav fvdaihtssg wtvlqkalgm skpyghvdff pnggrnqpgc
      301 gglleidcdh grapiyyies ikyrhqcrfl sykceggkea frsgrckpga pdsemgyysl
      361 qargrglqfl ttneespfcq pleqeltepk htkq
//
LOCUS       XP_077504440             387 aa            linear   INV 25-NOV-2025
DEFINITION  pancreatic lipase-related protein 2-like [Amblyomma americanum].
ACCESSION   XP_077504440
VERSION     XP_077504440.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648314.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..387
                     /product="pancreatic lipase-related protein 2-like"
                     /calculated_mol_wt=42918
     Region          94..372
                     /region_name="Pancreat_lipase_like"
                     /note="Pancreatic lipase-like enzymes. Lipases are
                     esterases that can hydrolyze long-chain acyl-triglycerides
                     into di- and monoglycerides, glycerol, and free fatty
                     acids at a water/lipid interface. A typical feature of
                     lipases is 'interfacial activation,' the...; cd00707"
                     /db_xref="CDD:238363"
     Site            216..220
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238363"
     Site            order(218,246,314)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238363"
     CDS             1..387
                     /gene="LOC144114518"
                     /coded_by="XM_077648314.1:478..1641"
                     /db_xref="GeneID:144114518"
ORIGIN      
        1 maigwillga rlfapmltfa vlgashqtyt elgqsksgdv eivkaidydt evakqqrkki
       61 evlpekvcyt dvgcfsrwdr mthpvalpqd pqgvrtqfhf ynrenrdipa vitepgprsl
      121 vsqahffrep kklvvllhgf tqsvvslwlh htkaallkke dvnvilvdws ngcrspnyfs
      181 avgnsaligr qvsltlqslv rqfpdtvdpa rvhvigfslg gqasgfcgrh flnstgrrlg
      241 ritaldpagp lfedsdvrvs kadahfvdii htsygwnmln gdlgmqaatg hvdfypnfqe
      301 rqpgcdafsl scrhrrahvy fveslvpdsc kfvsrpclpd tadpqcdllp rgemgygseh
      361 apgrgeqflr tnyeppfcls talddta
//
LOCUS       XP_077504442             143 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114519 [Amblyomma americanum].
ACCESSION   XP_077504442
VERSION     XP_077504442.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648316.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..143
                     /product="uncharacterized protein LOC144114519"
                     /calculated_mol_wt=15689
     CDS             1..143
                     /gene="LOC144114519"
                     /coded_by="XM_077648316.1:448..879"
                     /db_xref="GeneID:144114519"
ORIGIN      
        1 mvaasttvaa dvrtscyssa fprqrkglrt plgefknrrt snrhdqcvds pskiiggsar
       61 klgyhravqk ivscaraatr qtrdahtkmd wlppsrssrn capcrccspr prqlgrartt
      121 pwpphcpscr ssdsscvvsw msa
//
LOCUS       XP_077504443             143 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114519 [Amblyomma americanum].
ACCESSION   XP_077504443
VERSION     XP_077504443.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648317.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..143
                     /product="uncharacterized protein LOC144114519"
                     /calculated_mol_wt=15689
     CDS             1..143
                     /gene="LOC144114519"
                     /coded_by="XM_077648317.1:448..879"
                     /db_xref="GeneID:144114519"
ORIGIN      
        1 mvaasttvaa dvrtscyssa fprqrkglrt plgefknrrt snrhdqcvds pskiiggsar
       61 klgyhravqk ivscaraatr qtrdahtkmd wlppsrssrn capcrccspr prqlgrartt
      121 pwpphcpscr ssdsscvvsw msa
//
LOCUS       XP_077504444             143 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114519 [Amblyomma americanum].
ACCESSION   XP_077504444
VERSION     XP_077504444.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648318.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..143
                     /product="uncharacterized protein LOC144114519"
                     /calculated_mol_wt=15689
     CDS             1..143
                     /gene="LOC144114519"
                     /coded_by="XM_077648318.1:448..879"
                     /db_xref="GeneID:144114519"
ORIGIN      
        1 mvaasttvaa dvrtscyssa fprqrkglrt plgefknrrt snrhdqcvds pskiiggsar
       61 klgyhravqk ivscaraatr qtrdahtkmd wlppsrssrn capcrccspr prqlgrartt
      121 pwpphcpscr ssdsscvvsw msa
//
LOCUS       XP_077504445            1753 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114520 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504445
VERSION     XP_077504445.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648319.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1753
                     /product="uncharacterized protein LOC144114520 isoform X1"
                     /calculated_mol_wt=194276
     Region          21..124
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          624..843
                     /region_name="TBC"
                     /note="Domain in Tre-2, BUB2p, and Cdc16p. Probable
                     Rab-GAPs; smart00164"
                     /db_xref="CDD:214540"
     Region          <1015..>1192
                     /region_name="WD40_2"
                     /note="WD40 repeated domain; pfam19056"
                     /db_xref="CDD:465964"
     CDS             1..1753
                     /gene="LOC144114520"
                     /coded_by="XM_077648319.1:278..5539"
                     /db_xref="GeneID:144114520"
ORIGIN      
        1 msalamtaac agsngssvgp lsgylvqrpg galarlkgrr krwyvfdqhg cclhsyksdl
       61 daagrkaplf sidlrnaais flvdeencfv ihsgakeyvf vagshesmma wvmalqacrd
      121 lcreqepvda ahleeddiak rkislpagir inesgaprqs desqlqgaag hassahpeqc
      181 drklslqhfk akawpvtpfs lvnhhptied dcsaqpvtam citaeentae lkkmdsnvqp
      241 lsqnkiplvs tsssecvstv pgsiqccaqh qkeggavmst lrcrdasggr shyrraplrr
      301 vshhgstsdc esdsdavdrn gvgrdcggfs aamwerirfl rmassesakd sgrsqerags
      361 lsgnsissds aighgdstls lrlheleael matkcelaka lnretsqknt lieqenlise
      421 lqhqihclea sgddsrpntr gsnlnlqris dkccmlqshn rflneevqnl srllqqqqch
      481 aityqnclqd qekeidqlkr dyvflmqsai rmrsmegpev mevyfyggqr haarvlqllr
      541 earmsnpglp tydsqskvlq htdalgfrhh yteeslalhy icrqlhlhyq erlpsaahhl
      601 wrwrqylllc gdnlistkel kslvrqgvpa afrsqvwkal ytcrvadime dkgrnyysnl
      661 ccqasesevv sqnkrqisld llrtlpnnvr fsspdadgir klqevlqaic lhnpslgycq
      721 gmnflvgmcl lfmepedafw clvgiteryf tahyfdhslv gaqadqevlk tllrdklprl
      781 hrhlaqldie lctvtlnwfl aiffdsvpfe tllriwdcfl legpkvlfrf slailkmhee
      841 vlltkqdtvs imrqlkaiar lcydvdtlik vafedlepfp rrqdiaakqa cfqkilreqs
      901 kkreqekhsv lsrdsaaatd gscygdnali iecaaacdse tiwvchassn ltrlsrvnce
      961 dsvmyrlkie iearvicmha lgydvmllgt lshfvyayst rtrdmkweaq lnesvlslcs
     1021 yeeddqcqvf agladgtmav ienfsvhvdk pdvlyihigs spvtclklvd krlwcatgnr
     1081 iiilsartld tvdqfqvsps sldyisllqp cssedgvwma lrgssvlqlw daqaltckml
     1141 ydvrddrypr sprqqeedne lnparitall slpgsvlvgt aqgiliiyhl lprctatsdc
     1201 rtdsstegap pvkfggapsp ttgdrspape seartrndsg yvtstpkyas kcpgstlaay
     1261 ksddgvndts gsqhsfrtvi skstsclsss prntsedess pdskrhdgkk vnglkedgda
     1321 tqeeagiqks dvrrdrardk eitrdswqak gkssgssasp rkevessgei hmngsegkkk
     1381 nrstvedhar lpsspgrkig rhimdpseis icpvtevcae ntrkwslrta dscvsmielt
     1441 aplrkqssws sttattlshv lkrhnasvdc lpawlsrdtl sssltsdsyd fddffvqyad
     1501 dgmssltaqd srgipapacf aseeaqrlle rltvptvylt lgsheersst gsfwtenddg
     1561 aetsttheat tsewpegpsy dgnqpssdlg snisfssmdp qyayemlvqe kikisdkair
     1621 clvelrrkgg sivisgagcy gddesvlkwt eegeeklwtn dpvievcpyt ntikpspytr
     1681 srlprkvsaa qtngqartle aggaaamtng paggglmqrs rgrslldapk aatsmgarla
     1741 rmqsmfvkst eks
//
LOCUS       XP_077504446            1752 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114520 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504446
VERSION     XP_077504446.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648320.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1752
                     /product="uncharacterized protein LOC144114520 isoform X2"
                     /calculated_mol_wt=194148
     Region          21..124
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          623..842
                     /region_name="TBC"
                     /note="Domain in Tre-2, BUB2p, and Cdc16p. Probable
                     Rab-GAPs; smart00164"
                     /db_xref="CDD:214540"
     Region          <1014..>1191
                     /region_name="WD40_2"
                     /note="WD40 repeated domain; pfam19056"
                     /db_xref="CDD:465964"
     CDS             1..1752
                     /gene="LOC144114520"
                     /coded_by="XM_077648320.1:278..5536"
                     /db_xref="GeneID:144114520"
ORIGIN      
        1 msalamtaac agsngssvgp lsgylvqrpg galarlkgrr krwyvfdqhg cclhsyksdl
       61 daagrkaplf sidlrnaais flvdeencfv ihsgakeyvf vagshesmma wvmalqacrd
      121 lcreqepvda ahleeddiak rkislpagir inesgaprqs desqlqgaag hassahpeqc
      181 drklslqhfk akawpvtpfs lvnhhptied dcsaqpvtam citaeentae lkkmdsnvqp
      241 lsqnkiplvs tsssecvstv pgsiqccaqh qkeggavmst lrcrdasggr shyrraplrr
      301 vshhgstsdc esdsdavdrn gvgrdcggfs aamwerirfl rmassesakd sgrsqerags
      361 lsgnsissds aighgdstls lrlheleael matkcelaka lnretsqknt lieqenlise
      421 lqhqihclea sgddsrpntr gsnlnlqris dkccmlqshn rflneevqnl srllqqqqch
      481 aityqnclqd qekeidqlkr dyvflmqsai rmrsmegpev mevyfyggqr haarvlqllr
      541 earmsnpglp tydsqskvlq htdalgfrhh yteeslalhy icrqlhlhyq erlpsaahhl
      601 wrwrqylllc gdnlistelk slvrqgvpaa frsqvwkaly tcrvadimed kgrnyysnlc
      661 cqasesevvs qnkrqisldl lrtlpnnvrf sspdadgirk lqevlqaicl hnpslgycqg
      721 mnflvgmcll fmepedafwc lvgiteryft ahyfdhslvg aqadqevlkt llrdklprlh
      781 rhlaqldiel ctvtlnwfla iffdsvpfet llriwdcfll egpkvlfrfs lailkmheev
      841 lltkqdtvsi mrqlkaiarl cydvdtlikv afedlepfpr rqdiaakqac fqkilreqsk
      901 kreqekhsvl srdsaaatdg scygdnalii ecaaacdset iwvchassnl trlsrvnced
      961 svmyrlkiei earvicmhal gydvmllgtl shfvyaystr trdmkweaql nesvlslcsy
     1021 eeddqcqvfa gladgtmavi enfsvhvdkp dvlyihigss pvtclklvdk rlwcatgnri
     1081 iilsartldt vdqfqvspss ldyisllqpc ssedgvwmal rgssvlqlwd aqaltckmly
     1141 dvrddryprs prqqeednel nparitalls lpgsvlvgta qgiliiyhll prctatsdcr
     1201 tdsstegapp vkfggapspt tgdrspapes eartrndsgy vtstpkyask cpgstlaayk
     1261 sddgvndtsg sqhsfrtvis kstsclsssp rntsedessp dskrhdgkkv nglkedgdat
     1321 qeeagiqksd vrrdrardke itrdswqakg kssgssaspr kevessgeih mngsegkkkn
     1381 rstvedharl psspgrkigr himdpseisi cpvtevcaen trkwslrtad scvsmielta
     1441 plrkqsswss ttattlshvl krhnasvdcl pawlsrdtls ssltsdsydf ddffvqyadd
     1501 gmssltaqds rgipapacfa seeaqrller ltvptvyltl gsheersstg sfwtenddga
     1561 etsttheatt sewpegpsyd gnqpssdlgs nisfssmdpq yayemlvqek ikisdkairc
     1621 lvelrrkggs ivisgagcyg ddesvlkwte egeeklwtnd pvievcpytn tikpspytrs
     1681 rlprkvsaaq tngqartlea ggaaamtngp aggglmqrsr grslldapka atsmgarlar
     1741 mqsmfvkste ks
//
LOCUS       XP_077504447            1694 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114520 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504447
VERSION     XP_077504447.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648321.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1694
                     /product="uncharacterized protein LOC144114520 isoform X3"
                     /calculated_mol_wt=187973
     Region          21..124
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          565..784
                     /region_name="TBC"
                     /note="Domain in Tre-2, BUB2p, and Cdc16p. Probable
                     Rab-GAPs; smart00164"
                     /db_xref="CDD:214540"
     Region          <956..>1133
                     /region_name="WD40_2"
                     /note="WD40 repeated domain; pfam19056"
                     /db_xref="CDD:465964"
     CDS             1..1694
                     /gene="LOC144114520"
                     /coded_by="XM_077648321.1:278..5362"
                     /db_xref="GeneID:144114520"
ORIGIN      
        1 msalamtaac agsngssvgp lsgylvqrpg galarlkgrr krwyvfdqhg cclhsyksdl
       61 daagrkaplf sidlrnaais flvdeencfv ihsgakeyvf vagshesmma wvmalqacrd
      121 lcreqepvda ahleeddiak rkislpagir inesgaprqs desqlqgaag hassahpeqc
      181 drklslqhfk akawpvtpfs lvnhhptied dcsaqpvtam citaeentae lkkmdsnvqp
      241 lsqnkiplvs tsssecvstv pgsiqccaqh qkeggavmst lrcrdasggr shyrraplrr
      301 slsgnsissd saighgdstl slrlheleae lmatkcelak alnretsqkn tlieqenlis
      361 elqhqihcle asgddsrpnt rgsnlnlqri sdkccmlqsh nrflneevqn lsrllqqqqc
      421 haityqnclq dqekeidqlk rdyvflmqsa irmrsmegpe vmevyfyggq rhaarvlqll
      481 rearmsnpgl ptydsqskvl qhtdalgfrh hyteeslalh yicrqlhlhy qerlpsaahh
      541 lwrwrqylll cgdnlistke lkslvrqgvp aafrsqvwka lytcrvadim edkgrnyysn
      601 lccqasesev vsqnkrqisl dllrtlpnnv rfsspdadgi rklqevlqai clhnpslgyc
      661 qgmnflvgmc llfmepedaf wclvgitery ftahyfdhsl vgaqadqevl ktllrdklpr
      721 lhrhlaqldi elctvtlnwf laiffdsvpf etllriwdcf llegpkvlfr fslailkmhe
      781 evlltkqdtv simrqlkaia rlcydvdtli kvafedlepf prrqdiaakq acfqkilreq
      841 skkreqekhs vlsrdsaaat dgscygdnal iiecaaacds etiwvchass nltrlsrvnc
      901 edsvmyrlki eiearvicmh algydvmllg tlshfvyays trtrdmkwea qlnesvlslc
      961 syeeddqcqv fagladgtma vienfsvhvd kpdvlyihig sspvtclklv dkrlwcatgn
     1021 riiilsartl dtvdqfqvsp ssldyisllq pcssedgvwm alrgssvlql wdaqaltckm
     1081 lydvrddryp rsprqqeedn elnparital lslpgsvlvg taqgiliiyh llprctatsd
     1141 crtdsstega ppvkfggaps pttgdrspap eseartrnds gyvtstpkya skcpgstlaa
     1201 yksddgvndt sgsqhsfrtv iskstsclss sprntsedes spdskrhdgk kvnglkedgd
     1261 atqeeagiqk sdvrrdrard keitrdswqa kgkssgssas prkevessge ihmngsegkk
     1321 knrstvedha rlpsspgrki grhimdpsei sicpvtevca entrkwslrt adscvsmiel
     1381 taplrkqssw ssttattlsh vlkrhnasvd clpawlsrdt lsssltsdsy dfddffvqya
     1441 ddgmssltaq dsrgipapac faseeaqrll erltvptvyl tlgsheerss tgsfwtendd
     1501 gaetstthea ttsewpegps ydgnqpssdl gsnisfssmd pqyayemlvq ekikisdkai
     1561 rclvelrrkg gsivisgagc ygddesvlkw teegeeklwt ndpvievcpy tntikpspyt
     1621 rsrlprkvsa aqtngqartl eaggaaamtn gpaggglmqr srgrslldap kaatsmgarl
     1681 armqsmfvks teks
//
LOCUS       XP_077504448            1693 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114520 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077504448
VERSION     XP_077504448.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648322.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1693
                     /product="uncharacterized protein LOC144114520 isoform X4"
                     /calculated_mol_wt=187845
     Region          21..124
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          564..783
                     /region_name="TBC"
                     /note="Domain in Tre-2, BUB2p, and Cdc16p. Probable
                     Rab-GAPs; smart00164"
                     /db_xref="CDD:214540"
     Region          <955..>1132
                     /region_name="WD40_2"
                     /note="WD40 repeated domain; pfam19056"
                     /db_xref="CDD:465964"
     CDS             1..1693
                     /gene="LOC144114520"
                     /coded_by="XM_077648322.1:278..5359"
                     /db_xref="GeneID:144114520"
ORIGIN      
        1 msalamtaac agsngssvgp lsgylvqrpg galarlkgrr krwyvfdqhg cclhsyksdl
       61 daagrkaplf sidlrnaais flvdeencfv ihsgakeyvf vagshesmma wvmalqacrd
      121 lcreqepvda ahleeddiak rkislpagir inesgaprqs desqlqgaag hassahpeqc
      181 drklslqhfk akawpvtpfs lvnhhptied dcsaqpvtam citaeentae lkkmdsnvqp
      241 lsqnkiplvs tsssecvstv pgsiqccaqh qkeggavmst lrcrdasggr shyrraplrr
      301 slsgnsissd saighgdstl slrlheleae lmatkcelak alnretsqkn tlieqenlis
      361 elqhqihcle asgddsrpnt rgsnlnlqri sdkccmlqsh nrflneevqn lsrllqqqqc
      421 haityqnclq dqekeidqlk rdyvflmqsa irmrsmegpe vmevyfyggq rhaarvlqll
      481 rearmsnpgl ptydsqskvl qhtdalgfrh hyteeslalh yicrqlhlhy qerlpsaahh
      541 lwrwrqylll cgdnlistel kslvrqgvpa afrsqvwkal ytcrvadime dkgrnyysnl
      601 ccqasesevv sqnkrqisld llrtlpnnvr fsspdadgir klqevlqaic lhnpslgycq
      661 gmnflvgmcl lfmepedafw clvgiteryf tahyfdhslv gaqadqevlk tllrdklprl
      721 hrhlaqldie lctvtlnwfl aiffdsvpfe tllriwdcfl legpkvlfrf slailkmhee
      781 vlltkqdtvs imrqlkaiar lcydvdtlik vafedlepfp rrqdiaakqa cfqkilreqs
      841 kkreqekhsv lsrdsaaatd gscygdnali iecaaacdse tiwvchassn ltrlsrvnce
      901 dsvmyrlkie iearvicmha lgydvmllgt lshfvyayst rtrdmkweaq lnesvlslcs
      961 yeeddqcqvf agladgtmav ienfsvhvdk pdvlyihigs spvtclklvd krlwcatgnr
     1021 iiilsartld tvdqfqvsps sldyisllqp cssedgvwma lrgssvlqlw daqaltckml
     1081 ydvrddrypr sprqqeedne lnparitall slpgsvlvgt aqgiliiyhl lprctatsdc
     1141 rtdsstegap pvkfggapsp ttgdrspape seartrndsg yvtstpkyas kcpgstlaay
     1201 ksddgvndts gsqhsfrtvi skstsclsss prntsedess pdskrhdgkk vnglkedgda
     1261 tqeeagiqks dvrrdrardk eitrdswqak gkssgssasp rkevessgei hmngsegkkk
     1321 nrstvedhar lpsspgrkig rhimdpseis icpvtevcae ntrkwslrta dscvsmielt
     1381 aplrkqssws sttattlshv lkrhnasvdc lpawlsrdtl sssltsdsyd fddffvqyad
     1441 dgmssltaqd srgipapacf aseeaqrlle rltvptvylt lgsheersst gsfwtenddg
     1501 aetsttheat tsewpegpsy dgnqpssdlg snisfssmdp qyayemlvqe kikisdkair
     1561 clvelrrkgg sivisgagcy gddesvlkwt eegeeklwtn dpvievcpyt ntikpspytr
     1621 srlprkvsaa qtngqartle aggaaamtng paggglmqrs rgrslldapk aatsmgarla
     1681 rmqsmfvkst eks
//
LOCUS       XP_077504449             185 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114522 [Amblyomma americanum].
ACCESSION   XP_077504449
VERSION     XP_077504449.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648323.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..185
                     /product="uncharacterized protein LOC144114522"
                     /calculated_mol_wt=20389
     CDS             1..185
                     /gene="LOC144114522"
                     /coded_by="XM_077648323.1:141..698"
                     /db_xref="GeneID:144114522"
ORIGIN      
        1 mgsaktsdnn daeavpgsmr kqwvesssqg egstvkhqpe rlfqnvlaql tqsegpaask
       61 avpfvednla eqqeesmsee dslpeqlper lsgdvlaqia qieepvthkv vragvlkrka
      121 ekpvpaawts rnlclqvvei krpgpvseka rrfrheslfg grirrmpata flglqrkkav
      181 rrtkk
//
LOCUS       XP_077504450            1114 aa            linear   INV 25-NOV-2025
DEFINITION  COPII coat complex component secretory 24CD isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504450
VERSION     XP_077504450.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648324.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1114
                     /product="COPII coat complex component secretory 24CD
                     isoform X1"
                     /calculated_mol_wt=121956
     Region          299..1110
                     /region_name="COG5028"
                     /note="Vesicle coat complex COPII, subunit SEC24/subunit
                     SFB2/subunit SFB3 [Intracellular trafficking and
                     secretion]"
                     /db_xref="CDD:227361"
     CDS             1..1114
                     /gene="Sec24CD"
                     /coded_by="XM_077648324.1:141..3485"
                     /db_xref="GeneID:144114525"
ORIGIN      
        1 mnppqlgggg tvygghpfge phmqpvgppg fpqgsqapfm apaskayngv dgyaagaacv
       61 pggmplgmpp pkmgappgay mgssqpqqpa phmpqpppqh shqqqlpvhh qqqppplhqh
      121 aqhmqqppql qqppqlqqpp lpqqppmhqq qppqfqqhqp gappkgmplq pgqfvprqva
      181 pqesappgip rapgqapppp ghgppmaatp dqqlsnqmgn lqlsrrppqp mgprqpytsq
      241 ppypyppppg aappptqfyg pggvppqgtq angpgslgpp pgpgyggpaa ppmsashptm
      301 pappqqqprk ldpdqmpspg qqcspfntvp shiqvieddr kahggpfytn krgqvpplvt
      361 tdfivhdegi csprfmrsti ysvpctpeml rqtalpfalc ispfarqapg esalplsdfg
      421 edgpvrcnrc kaymcplmqf idggrrfqcv fckattevpp syfahldhlg qrsdkfqrpe
      481 lclgsyeiva tkeycknstf pqppaflfll dvsynsirng lvhifcshiq silhelprse
      541 geseskvrvg fityssvvhf ynlkaslaqp qmlvvpdvhd mfvplldgcl vsvqeasgli
      601 dslleqipvm fgetretetv lgsaiqagle alkaaecsgk lfvmhtslpi adapgklknr
      661 ddrkllgtdk eksvltpqsp fynqlgqecv aagccvdlfl fhnayvdvat vgqvakmtgg
      721 qvykytyfqs tadkalvqre snvqglqedv egarfledlr rdvsqptafd avmrvrtstg
      781 irptdfygny ymanttdvel aavdcnkcva veikyddkls eeegafvqva llyttcggqr
      841 rlrihnlala tcsqmadmyr ncdldtivnf lskqavrqll eqsprqvrdg cigrgaqmla
      901 ayrkhcasps ssgqlilpec mkllplylnc mlksdalagg pelttddrsf amlslcsmdv
      961 astvayfypr llplhdvdmd svelptalrc taekiredgc yllengihmf lwigmavdpd
     1021 flqnvfaqpv aarididkts llelntpisr rvrdiiarvr merqrcmrlt lvrqgdkmel
     1081 vfrqflvedr hgdlgpsyvd flchlhkeir nlls
//
LOCUS       XP_077504451            1114 aa            linear   INV 25-NOV-2025
DEFINITION  COPII coat complex component secretory 24CD isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504451
VERSION     XP_077504451.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648325.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1114
                     /product="COPII coat complex component secretory 24CD
                     isoform X1"
                     /calculated_mol_wt=121956
     Region          299..1110
                     /region_name="COG5028"
                     /note="Vesicle coat complex COPII, subunit SEC24/subunit
                     SFB2/subunit SFB3 [Intracellular trafficking and
                     secretion]"
                     /db_xref="CDD:227361"
     CDS             1..1114
                     /gene="Sec24CD"
                     /coded_by="XM_077648325.1:143..3487"
                     /db_xref="GeneID:144114525"
ORIGIN      
        1 mnppqlgggg tvygghpfge phmqpvgppg fpqgsqapfm apaskayngv dgyaagaacv
       61 pggmplgmpp pkmgappgay mgssqpqqpa phmpqpppqh shqqqlpvhh qqqppplhqh
      121 aqhmqqppql qqppqlqqpp lpqqppmhqq qppqfqqhqp gappkgmplq pgqfvprqva
      181 pqesappgip rapgqapppp ghgppmaatp dqqlsnqmgn lqlsrrppqp mgprqpytsq
      241 ppypyppppg aappptqfyg pggvppqgtq angpgslgpp pgpgyggpaa ppmsashptm
      301 pappqqqprk ldpdqmpspg qqcspfntvp shiqvieddr kahggpfytn krgqvpplvt
      361 tdfivhdegi csprfmrsti ysvpctpeml rqtalpfalc ispfarqapg esalplsdfg
      421 edgpvrcnrc kaymcplmqf idggrrfqcv fckattevpp syfahldhlg qrsdkfqrpe
      481 lclgsyeiva tkeycknstf pqppaflfll dvsynsirng lvhifcshiq silhelprse
      541 geseskvrvg fityssvvhf ynlkaslaqp qmlvvpdvhd mfvplldgcl vsvqeasgli
      601 dslleqipvm fgetretetv lgsaiqagle alkaaecsgk lfvmhtslpi adapgklknr
      661 ddrkllgtdk eksvltpqsp fynqlgqecv aagccvdlfl fhnayvdvat vgqvakmtgg
      721 qvykytyfqs tadkalvqre snvqglqedv egarfledlr rdvsqptafd avmrvrtstg
      781 irptdfygny ymanttdvel aavdcnkcva veikyddkls eeegafvqva llyttcggqr
      841 rlrihnlala tcsqmadmyr ncdldtivnf lskqavrqll eqsprqvrdg cigrgaqmla
      901 ayrkhcasps ssgqlilpec mkllplylnc mlksdalagg pelttddrsf amlslcsmdv
      961 astvayfypr llplhdvdmd svelptalrc taekiredgc yllengihmf lwigmavdpd
     1021 flqnvfaqpv aarididkts llelntpisr rvrdiiarvr merqrcmrlt lvrqgdkmel
     1081 vfrqflvedr hgdlgpsyvd flchlhkeir nlls
//
LOCUS       XP_077504452            1113 aa            linear   INV 25-NOV-2025
DEFINITION  COPII coat complex component secretory 24CD isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504452
VERSION     XP_077504452.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648326.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1113
                     /product="COPII coat complex component secretory 24CD
                     isoform X2"
                     /calculated_mol_wt=121828
     Region          262..1109
                     /region_name="COG5028"
                     /note="Vesicle coat complex COPII, subunit SEC24/subunit
                     SFB2/subunit SFB3 [Intracellular trafficking and
                     secretion]"
                     /db_xref="CDD:227361"
     CDS             1..1113
                     /gene="Sec24CD"
                     /coded_by="XM_077648326.1:141..3482"
                     /db_xref="GeneID:144114525"
ORIGIN      
        1 mnppqlgggg tvygghpfge phmqpvgppg fpqgsqapfm apaskayngv dgyaagaacv
       61 pggmplgmpp pkmgappgay mgssqpqqpa phmpqpppqh shqqqlpvhh qqqppplhqh
      121 aqhmqqppql qqppqlqqpp lpqqppmhqq qppqfqqhqp gappkgmplq pgqfvprqva
      181 pqesappgip rapgqapppp ghgppmaatp dqlsnqmgnl qlsrrppqpm gprqpytsqp
      241 pypyppppga appptqfygp ggvppqgtqa ngpgslgppp gpgyggpaap pmsashptmp
      301 appqqqprkl dpdqmpspgq qcspfntvps hiqvieddrk ahggpfytnk rgqvpplvtt
      361 dfivhdegic sprfmrstiy svpctpemlr qtalpfalci spfarqapge salplsdfge
      421 dgpvrcnrck aymcplmqfi dggrrfqcvf ckattevpps yfahldhlgq rsdkfqrpel
      481 clgsyeivat keycknstfp qppaflflld vsynsirngl vhifcshiqs ilhelprseg
      541 eseskvrvgf ityssvvhfy nlkaslaqpq mlvvpdvhdm fvplldgclv svqeasglid
      601 slleqipvmf getretetvl gsaiqaglea lkaaecsgkl fvmhtslpia dapgklknrd
      661 drkllgtdke ksvltpqspf ynqlgqecva agccvdlflf hnayvdvatv gqvakmtggq
      721 vykytyfqst adkalvqres nvqglqedve garfledlrr dvsqptafda vmrvrtstgi
      781 rptdfygnyy manttdvela avdcnkcvav eikyddklse eegafvqval lyttcggqrr
      841 lrihnlalat csqmadmyrn cdldtivnfl skqavrqlle qsprqvrdgc igrgaqmlaa
      901 yrkhcaspss sgqlilpecm kllplylncm lksdalaggp elttddrsfa mlslcsmdva
      961 stvayfyprl lplhdvdmds velptalrct aekiredgcy llengihmfl wigmavdpdf
     1021 lqnvfaqpva arididktsl lelntpisrr vrdiiarvrm erqrcmrltl vrqgdkmelv
     1081 frqflvedrh gdlgpsyvdf lchlhkeirn lls
//
LOCUS       XP_077504453            1101 aa            linear   INV 25-NOV-2025
DEFINITION  COPII coat complex component secretory 24CD isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504453
VERSION     XP_077504453.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648327.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1101
                     /product="COPII coat complex component secretory 24CD
                     isoform X3"
                     /calculated_mol_wt=120573
     Region          299..1097
                     /region_name="COG5028"
                     /note="Vesicle coat complex COPII, subunit SEC24/subunit
                     SFB2/subunit SFB3 [Intracellular trafficking and
                     secretion]"
                     /db_xref="CDD:227361"
     CDS             1..1101
                     /gene="Sec24CD"
                     /coded_by="XM_077648327.1:141..3446"
                     /db_xref="GeneID:144114525"
ORIGIN      
        1 mnppqlgggg tvygghpfge phmqpvgppg fpqgsqapfm apaskayngv dgyaagaacv
       61 pggmplgmpp pkmgappgay mgssqpqqpa phmpqpppqh shqqqlpvhh qqqppplhqh
      121 aqhmqqppql qqppqlqqpp lpqqppmhqq qppqfqqhqp gappkgmplq pgqfvprqva
      181 pqesappgip rapgqapppp ghgppmaatp dqqlsnqmgn lqlsrrppqp mgprqpytsq
      241 ppypyppppg aappptqfyg pggvppqgtq angpgslgpp pgpgyggpaa ppmsashptm
      301 pappqqqprk ldpdqmpspi qvieddrkah ggpfytnkrg qvpplvttdf ivhdegicsp
      361 rfmrstiysv pctpemlrqt alpfalcisp farqapgesa lplsdfgedg pvrcnrckay
      421 mcplmqfidg grrfqcvfck attevppsyf ahldhlgqrs dkfqrpelcl gsyeivatke
      481 ycknstfpqp paflflldvs ynsirnglvh ifcshiqsil helprseges eskvrvgfit
      541 yssvvhfynl kaslaqpqml vvpdvhdmfv plldgclvsv qeasglidsl leqipvmfge
      601 tretetvlgs aiqaglealk aaecsgklfv mhtslpiada pgklknrddr kllgtdkeks
      661 vltpqspfyn qlgqecvaag ccvdlflfhn ayvdvatvgq vakmtggqvy kytyfqstad
      721 kalvqresnv qglqedvega rfledlrrdv sqptafdavm rvrtstgirp tdfygnyyma
      781 nttdvelaav dcnkcvavei kyddklseee gafvqvally ttcggqrrlr ihnlalatcs
      841 qmadmyrncd ldtivnflsk qavrqlleqs prqvrdgcig rgaqmlaayr khcaspsssg
      901 qlilpecmkl lplylncmlk sdalaggpel ttddrsfaml slcsmdvast vayfyprllp
      961 lhdvdmdsve lptalrctae kiredgcyll engihmflwi gmavdpdflq nvfaqpvaar
     1021 ididktslle lntpisrrvr diiarvrmer qrcmrltlvr qgdkmelvfr qflvedrhgd
     1081 lgpsyvdflc hlhkeirnll s
//
LOCUS       XP_077504455            1100 aa            linear   INV 25-NOV-2025
DEFINITION  COPII coat complex component secretory 24CD isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077504455
VERSION     XP_077504455.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648329.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1100
                     /product="COPII coat complex component secretory 24CD
                     isoform X4"
                     /calculated_mol_wt=120445
     Region          298..1096
                     /region_name="COG5028"
                     /note="Vesicle coat complex COPII, subunit SEC24/subunit
                     SFB2/subunit SFB3 [Intracellular trafficking and
                     secretion]"
                     /db_xref="CDD:227361"
     CDS             1..1100
                     /gene="Sec24CD"
                     /coded_by="XM_077648329.1:141..3443"
                     /db_xref="GeneID:144114525"
ORIGIN      
        1 mnppqlgggg tvygghpfge phmqpvgppg fpqgsqapfm apaskayngv dgyaagaacv
       61 pggmplgmpp pkmgappgay mgssqpqqpa phmpqpppqh shqqqlpvhh qqqppplhqh
      121 aqhmqqppql qqppqlqqpp lpqqppmhqq qppqfqqhqp gappkgmplq pgqfvprqva
      181 pqesappgip rapgqapppp ghgppmaatp dqlsnqmgnl qlsrrppqpm gprqpytsqp
      241 pypyppppga appptqfygp ggvppqgtqa ngpgslgppp gpgyggpaap pmsashptmp
      301 appqqqprkl dpdqmpspiq vieddrkahg gpfytnkrgq vpplvttdfi vhdegicspr
      361 fmrstiysvp ctpemlrqta lpfalcispf arqapgesal plsdfgedgp vrcnrckaym
      421 cplmqfidgg rrfqcvfcka ttevppsyfa hldhlgqrsd kfqrpelclg syeivatkey
      481 cknstfpqpp aflflldvsy nsirnglvhi fcshiqsilh elprsegese skvrvgfity
      541 ssvvhfynlk aslaqpqmlv vpdvhdmfvp lldgclvsvq easglidsll eqipvmfget
      601 retetvlgsa iqaglealka aecsgklfvm htslpiadap gklknrddrk llgtdkeksv
      661 ltpqspfynq lgqecvaagc cvdlflfhna yvdvatvgqv akmtggqvyk ytyfqstadk
      721 alvqresnvq glqedvegar fledlrrdvs qptafdavmr vrtstgirpt dfygnyyman
      781 ttdvelaavd cnkcvaveik yddklseeeg afvqvallyt tcggqrrlri hnlalatcsq
      841 madmyrncdl dtivnflskq avrqlleqsp rqvrdgcigr gaqmlaayrk hcaspsssgq
      901 lilpecmkll plylncmlks dalaggpelt tddrsfamls lcsmdvastv ayfyprllpl
      961 hdvdmdsvel ptalrctaek iredgcylle ngihmflwig mavdpdflqn vfaqpvaari
     1021 didktsllel ntpisrrvrd iiarvrmerq rcmrltlvrq gdkmelvfrq flvedrhgdl
     1081 gpsyvdflch lhkeirnlls
//
LOCUS       XP_077504456            1101 aa            linear   INV 25-NOV-2025
DEFINITION  COPII coat complex component secretory 24CD isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504456
VERSION     XP_077504456.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648330.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1101
                     /product="COPII coat complex component secretory 24CD
                     isoform X3"
                     /calculated_mol_wt=120573
     Region          299..1097
                     /region_name="COG5028"
                     /note="Vesicle coat complex COPII, subunit SEC24/subunit
                     SFB2/subunit SFB3 [Intracellular trafficking and
                     secretion]"
                     /db_xref="CDD:227361"
     CDS             1..1101
                     /gene="Sec24CD"
                     /coded_by="XM_077648330.1:318..3623"
                     /db_xref="GeneID:144114525"
ORIGIN      
        1 mnppqlgggg tvygghpfge phmqpvgppg fpqgsqapfm apaskayngv dgyaagaacv
       61 pggmplgmpp pkmgappgay mgssqpqqpa phmpqpppqh shqqqlpvhh qqqppplhqh
      121 aqhmqqppql qqppqlqqpp lpqqppmhqq qppqfqqhqp gappkgmplq pgqfvprqva
      181 pqesappgip rapgqapppp ghgppmaatp dqqlsnqmgn lqlsrrppqp mgprqpytsq
      241 ppypyppppg aappptqfyg pggvppqgtq angpgslgpp pgpgyggpaa ppmsashptm
      301 pappqqqprk ldpdqmpspi qvieddrkah ggpfytnkrg qvpplvttdf ivhdegicsp
      361 rfmrstiysv pctpemlrqt alpfalcisp farqapgesa lplsdfgedg pvrcnrckay
      421 mcplmqfidg grrfqcvfck attevppsyf ahldhlgqrs dkfqrpelcl gsyeivatke
      481 ycknstfpqp paflflldvs ynsirnglvh ifcshiqsil helprseges eskvrvgfit
      541 yssvvhfynl kaslaqpqml vvpdvhdmfv plldgclvsv qeasglidsl leqipvmfge
      601 tretetvlgs aiqaglealk aaecsgklfv mhtslpiada pgklknrddr kllgtdkeks
      661 vltpqspfyn qlgqecvaag ccvdlflfhn ayvdvatvgq vakmtggqvy kytyfqstad
      721 kalvqresnv qglqedvega rfledlrrdv sqptafdavm rvrtstgirp tdfygnyyma
      781 nttdvelaav dcnkcvavei kyddklseee gafvqvally ttcggqrrlr ihnlalatcs
      841 qmadmyrncd ldtivnflsk qavrqlleqs prqvrdgcig rgaqmlaayr khcaspsssg
      901 qlilpecmkl lplylncmlk sdalaggpel ttddrsfaml slcsmdvast vayfyprllp
      961 lhdvdmdsve lptalrctae kiredgcyll engihmflwi gmavdpdflq nvfaqpvaar
     1021 ididktslle lntpisrrvr diiarvrmer qrcmrltlvr qgdkmelvfr qflvedrhgd
     1081 lgpsyvdflc hlhkeirnll s
//
LOCUS       XP_077504457             301 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114526 [Amblyomma americanum].
ACCESSION   XP_077504457
VERSION     XP_077504457.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648331.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..301
                     /product="uncharacterized protein LOC144114526"
                     /calculated_mol_wt=32954
     Region          6..>66
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..301
                     /gene="LOC144114526"
                     /coded_by="XM_077648331.1:1..906"
                     /db_xref="GeneID:144114526"
ORIGIN      
        1 magltwsdqe tyeliriwaa drdlldgtsr nnkvyesmaa qmrslgylrt alqvkekmkr
       61 lrkeykygkr skkiatyhea latvlaqgak ggalfiksev deslgseefa gtdsetnyme
      121 dgpgcsappa egngnderaq gffrgsnqgl dysrspgspa vecatalrfp adnfptittt
      181 taepgdgarf paagaggpra alkrklpdap spptlaastl tdlitrgidk fvelehkrmr
      241 leldllerlr heelerlerm rreemdhemr lmsvlaslfn nnnssssvnl nsssseppag
      301 s
//
LOCUS       XP_077504458             992 aa            linear   INV 25-NOV-2025
DEFINITION  integrator complex subunit 8-like [Amblyomma americanum].
ACCESSION   XP_077504458
VERSION     XP_077504458.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648332.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..992
                     /product="integrator complex subunit 8-like"
                     /calculated_mol_wt=110274
     CDS             1..992
                     /gene="LOC144114527"
                     /coded_by="XM_077648332.1:106..3084"
                     /db_xref="GeneID:144114527"
ORIGIN      
        1 metcedplpf srpnnsyvis wldfllddsl lekhlssekp dpspfelviq flanatdppl
       61 qgpnggamap gnglqgivgl dgtsiepgsp eeepgnrksq plkllalkaa aflgwnmdll
      121 eqklpltmqq tliaelqkaa gcsdlneeqr lsnpdaafac miksrwvvht lmrssvpvks
      181 skgiavqlpg qvdptvvtpe madaitkkce eeveeavafl ekllsrratg glsvrvptsq
      241 sfsvvkaell dpehdwekgv iataddvccq vsyelgafhf ykenyrrage lfetalslhs
      301 kvkkmvvcql dmerlkgfsm acrltatkks gtlldklkat lhgtckatvp vllednikre
      361 iplweremae lyvqqnfagt evaaqvflcn avrriisgel fisgaqvnfa tldkgcimfl
      421 nevlnavipt ldekemgfmr nfvgyvcnas advakvlscs gvlrkcfpse qvtppaevpp
      481 kiplervsfa enaatnigrm eralvashdp eeivslikll qgvsppvrlt slsshwqltp
      541 nlrefhrglp qqwqermfvl lakalelrsl klygpslllf haveaelrlp qwrrlaallv
      601 aemrytealq asdtlplpag tqrrptdliq lahtsllayy aeddkdyrpc qemaelcctl
      661 llnlsewdif seienkqras vgvfelskal aavckdvsmn kltrsiaqel wdlllsmlvt
      721 pgtpqnkkas itagkdggqr datatptlsr htfhsflqql kdnmaltill sclakmynil
      781 rddsgsevcl eypqlwpaai sspsnycrat amdvfhatlq hclatnnsha gwvkvladfc
      841 yaqghhsaal khylaallms tdyftqpppr sladdlmykk mshccsklqc htqaalfcql
      901 meepdynaaf kalnerqcqd scdslyehvf ditlleflvn lhtrrgeles rqkalqcigl
      961 lelnasnnee iqreaanvrr gdflrvmarq yl
//
LOCUS       XP_077504459             153 aa            linear   INV 25-NOV-2025
DEFINITION  ribosomal protein L14 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504459
VERSION     XP_077504459.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648333.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..153
                     /product="ribosomal protein L14 isoform X1"
                     /calculated_mol_wt=17498
     Region          1..124
                     /region_name="PTZ00065"
                     /note="60S ribosomal protein L14; Provisional"
                     /db_xref="CDD:240252"
     CDS             1..153
                     /gene="RpL14"
                     /coded_by="XM_077648333.1:112..573"
                     /db_xref="GeneID:144114528"
ORIGIN      
        1 mtlrtfvevg rvaylthgky rgrlcsivni vdqnravvdg petgvprqav afkhmrltrq
       61 kirvpmgtst kvvrkawkkn evsqkwnesa lakklaarrl kanmndfdrf klrrakqsln
      121 kvvrlrflkl kslskkagkk dreekakkaa pkk
//
LOCUS       XP_077504460             153 aa            linear   INV 25-NOV-2025
DEFINITION  ribosomal protein L14 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504460
VERSION     XP_077504460.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648334.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..153
                     /product="ribosomal protein L14 isoform X2"
                     /calculated_mol_wt=17498
     Region          1..124
                     /region_name="PTZ00065"
                     /note="60S ribosomal protein L14; Provisional"
                     /db_xref="CDD:240252"
     CDS             1..153
                     /gene="RpL14"
                     /coded_by="XM_077648334.1:17..478"
                     /db_xref="GeneID:144114528"
ORIGIN      
        1 mtlrtfvevg rvaylthgky rgrlcsivni vdqnravvdg petgvprqav afkhmrltrq
       61 kirvpmgtst kvvrkawkkn evsqkwnesa lakklaarrl kanmndfdrf klrrakqsln
      121 kvvrlrflkl kslskkagkk dreekakkaa pkk
//
LOCUS       XP_077504461              92 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114529 [Amblyomma americanum].
ACCESSION   XP_077504461
VERSION     XP_077504461.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648335.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..92
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..92
                     /product="uncharacterized protein LOC144114529"
                     /calculated_mol_wt=9763
     Region          2..>44
                     /region_name="SelK_SelG"
                     /note="Selenoprotein SelK_SelG; pfam10961"
                     /db_xref="CDD:463198"
     CDS             1..92
                     /gene="LOC144114529"
                     /coded_by="XM_077648335.1:182..460"
                     /db_xref="GeneID:144114529"
ORIGIN      
        1 mpyinergei lesrplwrig titeffqgla rmvglffqtl fmmdsssssr ssasrsggtn
       61 srrppgptrr rqigglggis sgtvdcplpg gg
//
LOCUS       XP_077504462             391 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: lysosomal aspartic protease-like [Amblyomma
            americanum].
ACCESSION   XP_077504462
VERSION     XP_077504462.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648336.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..391
                     /product="LOW QUALITY PROTEIN: lysosomal aspartic
                     protease-like"
                     /calculated_mol_wt=41771
     Region          23..>38
                     /region_name="A1_Propeptide"
                     /note="A1 Propeptide; pfam07966"
                     /db_xref="CDD:462326"
     Region          60..389
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(89,91,93,131..133,179)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133137"
     Site            89..91
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133137"
     Site            89
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133137"
     Site            order(131..134,138..141)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133137"
     CDS             1..391
                     /gene="LOC144114530"
                     /coded_by="XM_077648336.1:91..1266"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 1 base in 1 codon"
                     /db_xref="GeneID:144114530"
ORIGIN      
        1 mearlsaivv llalagaasa llrvplmkmk sardhfrevg tpinvtrgws lgsafggpvp
       61 eplknyldaq yygditlgtp pqvfrvvfdt gssnlwvpss kcpftniacm lhhkyyskrs
      121 styvkngtqf eirygsgsvk gelstdvfgl gdvrvqsqtf aeilreagla fiaakfdgil
      181 glgypqisvl gvppvfdnmv aqgvaakpvf svyldrnasd pnggevlfgg ideahytgni
      241 tyvpvtrkgy wqfhmdglkv gnnatfcngg ceaiadtgts liagptaeie klnlaigaap
      301 ftageylvsc ksiptlpkit fnlnghdfvl egkdyvlqvs qagiplclsg figldvpapl
      361 gplwixgdvf igryytifdr gndrvgfaes r
//
LOCUS       XP_077504463             172 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114532 [Amblyomma americanum].
ACCESSION   XP_077504463
VERSION     XP_077504463.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648337.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..172
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..172
                     /product="uncharacterized protein LOC144114532"
                     /calculated_mol_wt=14565
     CDS             1..172
                     /gene="LOC144114532"
                     /coded_by="XM_077648337.1:107..625"
                     /db_xref="GeneID:144114532"
ORIGIN      
        1 mklllaltvc glavslatag flsgggggfg gggfgggggg pwrggggfgg fggggggpwr
       61 gggglggfgg gkggfggggf ggggpwrsgg gfggggggpw rggggglggf ggglkggfgg
      121 ggfggggpwr ggggfggfgg gggpwrgggg gfggggwrag gggfggggrg ww
//
LOCUS       XP_077504464             389 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114533 [Amblyomma americanum].
ACCESSION   XP_077504464
VERSION     XP_077504464.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648338.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..389
                     /product="uncharacterized protein LOC144114533"
                     /calculated_mol_wt=41356
     CDS             1..389
                     /gene="LOC144114533"
                     /coded_by="XM_077648338.1:123..1292"
                     /db_xref="GeneID:144114533"
ORIGIN      
        1 mqvflvvlll laaslleara gqhdgwamta patsgapast nvqgfyvlrr parydspqqp
       61 apsepvtsga slrqwsnnfp astqplatnp qyydtapatq rapaaakwkf sgstdawear
      121 nngrngqtrs terfevrktn grshrkpaas skstasagwa lssapskskp knilvidagy
      181 ttgniqdelf ngwpmskpng ngwqtaassg wqaansngwq aassqgwqss snngwqapsn
      241 deqvytvkta sldhaaglea dtppffsgag ptsgnghggs pdkhvyivkt ishspepiaa
      301 amapawqaee wsdapmkwps ngwngametm dmpmdqqngg igqqllallg qkqefilktl
      361 psllpspqqs apsdwpsdsa kpskknpwg
//
LOCUS       XP_077504465             828 aa            linear   INV 25-NOV-2025
DEFINITION  hemicentin-2-like [Amblyomma americanum].
ACCESSION   XP_077504465
VERSION     XP_077504465.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648339.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..828
                     /product="hemicentin-2-like"
                     /calculated_mol_wt=90235
     Region          98..196
                     /region_name="V-set"
                     /note="Immunoglobulin V-set domain; pfam07686"
                     /db_xref="CDD:462230"
     Region          339..343
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          <341..407
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          353..357
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          385..389
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          399..404
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          415..418
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          436..510
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          447..451
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          461..465
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          482..486
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          496..501
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          510..513
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          525..598
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     CDS             1..828
                     /gene="LOC144114534"
                     /coded_by="XM_077648339.1:279..2765"
                     /db_xref="GeneID:144114534"
ORIGIN      
        1 mllrcaklrg ssghlrwasp prktfsceqs tfsrvmpgta casrlltrrr wnarvvyflt
       61 llsslttsae vvggptsylg dqsvpwvrst prvkypgspe avqalrgeta rlpcrtelaa
      121 eteqplllqw yrgpqliysv dsenvplvqa rhtvhsgare errlhfsvgr rpyrlslghv
      181 qpedegvyyc lvafpsgtsi nasvslvvvv ppakpviryv shngvkeptg svigpfnege
      241 rlvllcevlg ghpnptvtwh vghsaihrav tqlqgdktls wieverlers hfrkvvtcaa
      301 vsgtgtqpvk ssvtvdlhlk pqsvtirgah gplsanypae vecealgsrp panltwdldg
      361 kvltssaleg ggarsgaaan dvsvsvvrft psprhngrpl rcraanpemp ddviedswtl
      421 dvhykpkvhl tlgshprqwn iveghdlyle crvdanpsig dvawrldgrd lspgrhvims
      481 nqslvlqrvh rdssgsytcv asnrigetes qplkitvkha pacnrsqttv yaasrheevq
      541 iicdvsaepk dvkfmwtfns svskhevssf tssggrsvlf ytarkdsdyg tfqclannsi
      601 gamatpcyfq ivragplssm yncvvsnvtd aglqvdcqrr gtpagdangg slppdlvaae
      661 astfrqrsaf qleirdalld rlvfnatses psfavsslsp gteyvvafyl vnqngkfklv
      721 qlktstlfas eerlrsgveh rkpccaslgl litaavvlaf llvvtallik yrtrlkldng
      781 srkqdsrpeg gaeegsemgd vnpeckyvlp aesgkqlsss kespltyv
//
LOCUS       XP_077504467             164 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114536 [Amblyomma americanum].
ACCESSION   XP_077504467
VERSION     XP_077504467.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648341.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..164
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..164
                     /product="uncharacterized protein LOC144114536"
                     /calculated_mol_wt=17292
     CDS             1..164
                     /gene="LOC144114536"
                     /coded_by="XM_077648341.1:151..645"
                     /db_xref="GeneID:144114536"
ORIGIN      
        1 mklllvaaal vglsaaqive rrviknpdgs tthmessswg hssfnqeaad tdgiisgrsg
       61 yadtsgvnyd rhytvdrngq rrfvdpkesk lkgppptftm prgtfgaggd sdflagrsgf
      121 gnlpgfgnlp gfggnvpgfd gnfpgfggnp tldlwrlfpp glrf
//
LOCUS       XP_077504468             559 aa            linear   INV 25-NOV-2025
DEFINITION  putative 4-coumarate--CoA ligase 1 [Amblyomma americanum].
ACCESSION   XP_077504468
VERSION     XP_077504468.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648342.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..559
                     /product="putative 4-coumarate--CoA ligase 1"
                     /calculated_mol_wt=60533
     Region          62..541
                     /region_name="AFD_class_I"
                     /note="Adenylate forming domain, Class I superfamily;
                     cl17068"
                     /db_xref="CDD:473059"
     Site            order(205,208..213,215..216)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341228"
     Site            order(208,324..325,347..352,431,443,446,457,541)
                     /site_type="other"
                     /note="AMP binding site [chemical binding]"
                     /db_xref="CDD:341228"
     Site            order(208,250..251,297,299..300,303,324..325,347..352,431,
                     443,446,454..457,522)
                     /site_type="active"
                     /db_xref="CDD:341228"
     Site            order(250,299..300,303,324,454..456,515,522)
                     /site_type="other"
                     /note="CoA binding site [chemical binding]"
                     /db_xref="CDD:341228"
     CDS             1..559
                     /gene="LOC144114537"
                     /coded_by="XM_077648342.1:192..1871"
                     /db_xref="GeneID:144114537"
ORIGIN      
        1 mpvprenrgr algarsttat sgmaaviedc vvrssnqdly ipdvdlgtff rgccrkykdr
       61 tavvdaatge sctyrqledl crrvaaglrn lgfqpgdlag ihsgvnvdli tafygtvlag
      121 grvvfakgnl tqrevqyqfs dsapklvfcd dasaektkaa ceavpsvktl vtlgehegmv
      181 rfstlkefqa gysaagidpr gvvailyssg ttglpkgvmm shrnlisqll lsgddgmrli
      241 rnsdtmlgta pfthvsglwl ystcftvgas vvvtaisdpd svlkaierhk atlmlqfpty
      301 vqkmvqcahi dkydvssmkt llvggsttpt amakaiidkf klkafrhvfg msetcgavtv
      361 tessiedyes vgkpapmtyi kvvdvetrek lgprqpgeic vkgpycclgy lnkpeatknl
      421 ydddgfilsg digyyteegk lfvvdrikel ikcmdqqvsp aelegllvqh ravkevvvvg
      481 vphreygeaa rafvvlfqgt aasdalkkdi aklvadqsaf hkhlhggief vesipksdtg
      541 knlrralrds flknkdagi
//
LOCUS       XP_077504469            1052 aa            linear   INV 25-NOV-2025
DEFINITION  GTPase activating protein and centrosome-associated [Amblyomma
            americanum].
ACCESSION   XP_077504469
VERSION     XP_077504469.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648343.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1052
                     /product="GTPase activating protein and
                     centrosome-associated"
                     /calculated_mol_wt=117181
     Region          102..234
                     /region_name="PTB_Rab6GAP"
                     /note="GTPase activating protein for Rab 6
                     Phosphotyrosine-binding (PTB) domain; cd01211"
                     /db_xref="CDD:269922"
     Site            order(106,185,208)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:269922"
     Site            order(171..176,192,218,222)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:269922"
     Region          282..408
                     /region_name="DUF3694"
                     /note="Kinesin protein; pfam12473"
                     /db_xref="CDD:463599"
     Region          534..738
                     /region_name="TBC"
                     /note="Domain in Tre-2, BUB2p, and Cdc16p. Probable
                     Rab-GAPs; smart00164"
                     /db_xref="CDD:214540"
     Region          <755..>1007
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..1052
                     /gene="GAPcenA"
                     /coded_by="XM_077648343.1:297..3455"
                     /db_xref="GeneID:144114539"
ORIGIN      
        1 meeeassdsl atseeyelvc peaprlsiag ngsqdqlqsh lgellaegpv hrelgrgadd
       61 qggegemkta sesspeetmp epkprsssle sgpevsgheq afeevaylgc aainaprsev
      121 eiqnnmailn dqspeqhiqv alvvppdseg vvtvyesstr sevasfpvqr ilfcargiqg
      181 saeqncfaft cshgnsaeea ifqchvfrct kggpaavhrv lqsfagafrr mprqppgalg
      241 gspplssvgt gvpnneqvht feasldvree dtrgnfsaca rdkdcfkfrc ntekmvqvti
      301 qqttatqelg iercfgmlic pgrhvkhsdm qligmvsmak stvgteptay qitgrwnpqe
      361 pafellntet srdtrvymtv avdlvvtgvr epvrfvfetk akifpqaekf wyfgrkpfhe
      421 efflrlrevd peearqghrw evvgvssgsq irrkaalsla ldqhspcqep spesasgdid
      481 sdldepllsg tgevskdcsa delegwaqvl nrwrqaglea gppktlgplv raagvpealr
      541 gevwqllaga aqdpqaaqay rlllardcsc egviqrdlhr tfpaheffrd aagqdalyki
      601 ckayavqdpe vgycqglsfl aaalllhmpe eqafgvfckv mseyglrdlf rnsfeclhlk
      661 lfqlerlmed qlpqlyahfv dlgveahmfg sqwfltlfta kfplhvvffi ldlflldgmd
      721 tlfqvavall tlscrdllal dfegvlkhfr vavpkkyrse eaarlllkta vgikvkklkr
      781 yereyqlhke qerqmedpvq vlqkekqrlm esnlrleren delahelvts kiqlrkdlda
      841 aeekadilek elqtatsala eleeekrrld tevgqlkemc rrelqtaeae avrnttilge
      901 ykqicrhlsd qiereqrrak eslrvlqata cpscaalver aidehgwpql kkssagdcgr
      961 sqeiaqlrql elelaqtkla lveaqcrnqd lthqlscasa elqatkntwl hktlssirdv
     1021 arkepsssgl skdskdskds kdskdskdps vp
//
LOCUS       XP_077504470             337 aa            linear   INV 25-NOV-2025
DEFINITION  8-oxoguanine DNA glycosylase [Amblyomma americanum].
ACCESSION   XP_077504470
VERSION     XP_077504470.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648344.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..337
                     /product="8-oxoguanine DNA glycosylase"
                     /calculated_mol_wt=38108
     Region          5..328
                     /region_name="ogg"
                     /note="8-oxoguanine DNA-glycosylase (ogg); TIGR00588"
                     /db_xref="CDD:211589"
     CDS             1..337
                     /gene="Ogg1"
                     /coded_by="XM_077648344.1:129..1142"
                     /db_xref="GeneID:144114540"
ORIGIN      
        1 mvssgvikct trqlnldvtl tsgqafrwkk ndqsewmstf agrvwclrqd ddgnlhyrvl
       61 nipnnglpcp nfgssqtecr srkrrclevv ppsteeneel lrdyfqlgvd leglytewcl
      121 vdktfratar ylpgirvlrq eplealmafi cssnnnitri ssmveklctm ygtkifegke
      181 gsfyafptvs qmdqesvevk lreagfgyra kyvhgaakvl tsrgplwlqs lrgapyteah
      241 qqlmelpgvg akvadcvclm aldkseavpv dvhvwrmatq hylphlrslk nlsavaykei
      301 gdhfrerfgc yagwaqsvlf tsdlrrhska nshrcgd
//
LOCUS       XP_077504471             269 aa            linear   INV 25-NOV-2025
DEFINITION  N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 [Amblyomma
            americanum].
ACCESSION   XP_077504471
VERSION     XP_077504471.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648345.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..269
                     /product="N(G),N(G)-dimethylarginine
                     dimethylaminohydrolase 1"
                     /calculated_mol_wt=29484
     Region          4..>177
                     /region_name="DdaH"
                     /note="N-Dimethylarginine dimethylaminohydrolase [Amino
                     acid transport and metabolism]; COG1834"
                     /db_xref="CDD:441439"
     CDS             1..269
                     /gene="LOC144114541"
                     /coded_by="XM_077648345.1:34..843"
                     /db_xref="GeneID:144114541"
ORIGIN      
        1 mafgrythai vcrvpnsvkq casgvigdin velarkehee fcktlrsldl dvielqades
       61 tpdcafvddt aiicngaali crpangqrqk eldiiratik kelgiavidi rdpkaviegg
      121 nvlftgreff vgisprtnea garalaaafp efpvtpvklp krnhlkalls magpdvicls
      181 dspeaqavkr qvereatfqy qtvtlpeera agclyingrl lhrhefptan qefaqkidyd
      241 rvtlsvtels kasnglpsla llvrksrhh
//
LOCUS       XP_077504472             125 aa            linear   INV 25-NOV-2025
DEFINITION  prefoldin 6 [Amblyomma americanum].
ACCESSION   XP_077504472
VERSION     XP_077504472.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648346.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..125
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..125
                     /product="prefoldin 6"
                     /calculated_mol_wt=14455
     Region          12..112
                     /region_name="Prefoldin_6"
                     /note="Prefoldin subunit 6; cd23161"
                     /db_xref="CDD:467477"
     Site            order(12,15,23..29,31,59,69..70,72..74,76..78,80..81,
                     83..85,87..89,91..92,107..112)
                     /site_type="other"
                     /note="prefoldin beta subunit /TRiC interface [polypeptide
                     binding]"
                     /db_xref="CDD:467477"
     Region          12..49
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:467477"
     Site            order(35,38..39,41..42,44..46,48,52..67,70..71,78,82)
                     /site_type="other"
                     /note="prefoldin oligomer interface [polypeptide binding]"
                     /db_xref="CDD:467477"
     Region          69..112
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:467477"
     CDS             1..125
                     /gene="Pfdn6"
                     /coded_by="XM_077648346.1:96..473"
                     /db_xref="GeneID:144114543"
ORIGIN      
        1 meaihkalvq evekyktlqk dfqktfnlrq kldsqfnenc vvkeeldlle peagvyklig
       61 pvlikqgldd akqnvnkrlq yitaelkrhd tllkdlekkq eaqreaihkl eqqiqqsqgk
      121 gairm
//
LOCUS       XP_077504473             334 aa            linear   INV 25-NOV-2025
DEFINITION  atonal bHLH transcription factor 8-like protein net [Amblyomma
            americanum].
ACCESSION   XP_077504473
VERSION     XP_077504473.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648347.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..334
                     /product="atonal bHLH transcription factor 8-like protein
                     net"
                     /calculated_mol_wt=35870
     Region          222..289
                     /region_name="bHLH_TS_ATOH8"
                     /note="basic helix-loop-helix (bHLH) domain found in
                     protein atonal homolog 8 (ATOH8) and similar proteins;
                     cd11421"
                     /db_xref="CDD:381427"
     Site            order(230,234,236..238,240,245,263..266)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381427"
     Site            order(243..244,247..248,250..251,267,270,274,276..277,281,
                     283..284)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:381427"
     CDS             1..334
                     /gene="net"
                     /coded_by="XM_077648347.1:91..1095"
                     /db_xref="GeneID:144114544"
ORIGIN      
        1 msaassddsg velhkrrnkr ksseprrrrl lsgrprpcqd adtteddsae ergpapaqpa
       61 gresviregp prrpapdedl gldltttsns patsatsnta ggtegddecl dlrsshapvf
      121 esreagvvlf gghmalcrdq ssaqprswap dckrplaedp egslrskria lfdgastsfp
      181 sgssaprtrd tfdasdveli prtsgaspcl esasgrcaqr nykgmtrqrr ieanarersr
      241 vhtisaafea lrravpayad nqklsklail riaaayival arlneqdysc dqrapsfaec
      301 tdlctrtiqa egkarrrssa paaaaakdsl elss
//
LOCUS       XP_077504474            1241 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114545 [Amblyomma americanum].
ACCESSION   XP_077504474
VERSION     XP_077504474.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648348.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1241
                     /product="uncharacterized protein LOC144114545"
                     /calculated_mol_wt=134750
     Region          836..1134
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(843..846,849,851,869,871,901,918..921,999,
                     1003..1004,1006,1016..1017)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..1241
                     /gene="LOC144114545"
                     /coded_by="XM_077648348.1:1530..5255"
                     /db_xref="GeneID:144114545"
ORIGIN      
        1 mrsptkpvaw tllvwcllpp tllsataptt rgvaylaewp rkgrvatvel padfpigrpf
       61 fslcaavvpl tpaagpqetp aavptlrysl vsatrtlrpg arssrqsapf dvhavtgdvt
      121 ltdtlrengr penrpvewnl vvearveaeg fengsrtqlr vrllseeera kicasrrkvc
      181 fgeegykley sihevaragt vldvvrppai avvcpdseps yavrqdddnw lavdessgql
      241 lltrpvfwse spvagstavm arlvcslagh mnetakvalq vkdldnqppr aqtesslvcr
      301 ksvhslhkgd nldcvisvld qdstdannfs vrveqdnlnl feldglpfqt fitdkkmtfl
      361 nakvivrssg awfpgdkynf avvvedvglm rtdvpskvtf qievsnsstl rtplslrdty
      421 nvslsrnatv farvlssmta pasnhrfqlv adepspghdg taaahpwlav tprtgilyvs
      481 neqrlrdfna satsvllqas wlegehgpsr phlyrvevhi qdaeggshte rcegrlcgqq
      541 lseaqcsasc gpgtldgrcr wrhgsggrhl ttrystctgd pescpdgtcd elelkhptlc
      601 pqdcaehvvg eavagpsgrg iekamgicsc asaesctctk ppsrpdsrds vlavptwshg
      661 sdgaagadgq svfgqlsaat adldhrqepg gesvapaaaa lcgpgcvasl clagllvsvl
      721 alaalatlvr rrraaakhky lgsraslsvp sdyvdgrsaa saaaadagaq astpypqsat
      781 gspflaklqt syrrtrspae gpsllweerl pggqnaclpa sstevvhdqw efprenlale
      841 qclgegefgr vvratardia gvrglttvav kmlkgnwtpa eeqdllsefc mlkevnhpnv
      901 irllggcttk ggplyvivey aelgsllnvl rrsrhmaadv tvvcnptyma qgpattvsqe
      961 dvedrtepeg psysdllcya wqiakgmayl admklvhrdl aarnillaeg nvikisdfgl
     1021 srdvyegdty lkksrgrvpv kwmalesled qiytsksdvw sfgivlweiv tlgaspypgv
     1081 gpehlfrllk tgyrmhqpes csdemyriml scwqarpqdr pcfkelrqrl edilqdsasy
     1141 lelngpqnrs yynlnggapl mkplleehsd dsegsedpey gcmefvrrgs fsehsaseas
     1201 nvqlltkkng fpvdspvvcw larpevlsrd gagterpkql l
//
LOCUS       XP_077504476             621 aa            linear   INV 25-NOV-2025
DEFINITION  soluble guanylate cyclase 88E-like [Amblyomma americanum].
ACCESSION   XP_077504476
VERSION     XP_077504476.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648350.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..621
                     /product="soluble guanylate cyclase 88E-like"
                     /calculated_mol_wt=70659
     Region          2..163
                     /region_name="HNOB"
                     /note="Haem-NO-binding; pfam07700"
                     /db_xref="CDD:462233"
     Region          204..397
                     /region_name="HNOBA"
                     /note="Heme NO binding associated; pfam07701"
                     /db_xref="CDD:462234"
     Region          408..587
                     /region_name="Guanylate_cyc"
                     /note="Adenylate and Guanylate cyclase catalytic domain;
                     pfam00211"
                     /db_xref="CDD:425528"
     Site            order(417,419..424,459,461..463,466,529..531,535..536,
                     539..540,576)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(419,463)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(424,436,439..440,443,447,459..460,517,520,530..533,
                     536,576)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     CDS             1..621
                     /gene="LOC144114546"
                     /coded_by="XM_077648350.1:5..1870"
                     /db_xref="GeneID:144114546"
ORIGIN      
        1 mygmllesvq hyvrlqfgep lweaalaelg lhnfsthlvy pddsmtrlad acakrvpgws
       61 rdtflfffgr cfvrffshyg ydklirasgr hlrdflagid nlhhqirfsy prmrspslql
      121 lqehrggall lyrscrqglh fylvgqlvqv astfygmrlq vrvlsqrctp dgsqallrls
      181 fdnaaftlne qrrqraqegv slapvswgsl lclfpfgmlv drrmrvlwlg rrlrivfgqd
      241 deaaacpgrp vqelmrlhrp ivaftwenll anqqvvvele cifseqspvp arrlllkgqm
      301 rylqesdvil flgvpllsgl delqmaglyl edlcihdmtr divlaewqhg anlevsyreq
      361 erksrtleen lvrldawrqr sdellysmip ksvadrlrrg ahpvdtcetf dsvtvlfsel
      421 vnfpvacrhl spmqvvswvn atfslfdlit dryqvfkvet vgqvymlvsg aperredhae
      481 qvsaaaleml fafqgkrqpf deqgtflrig vhsgpvaagv vglktlrycl fgdtvntaar
      541 mqthgengri hisdscqrli rkkgfvtepr gkievkgkgk mrthwllegd vlstaasggh
      601 rrislgevgp ltrartkras k
//
LOCUS       XP_077504477             139 aa            linear   INV 25-NOV-2025
DEFINITION  inositol-3-phosphate synthase [Amblyomma americanum].
ACCESSION   XP_077504477
VERSION     XP_077504477.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648351.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..139
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..139
                     /product="inositol-3-phosphate synthase"
                     /calculated_mol_wt=15715
     Region          3..>92
                     /region_name="NAD_binding_5"
                     /note="Myo-inositol-1-phosphate synthase; cl00554"
                     /db_xref="CDD:469819"
     CDS             1..139
                     /gene="Inos"
                     /coded_by="XM_077648351.1:330..749"
                     /db_xref="GeneID:144114548"
ORIGIN      
        1 mvkveidspt vrytadliea dyvystsdak vegnvcrvrp rdvrmtfrtk rrvprlgvml
       61 vgiggnngst vvaatlankl glswrtkdkv qvrarehprs afwrtkwvva adrrkngvvi
      121 givpycyryr pvrgvhert
//
LOCUS       XP_077504478             139 aa            linear   INV 25-NOV-2025
DEFINITION  inositol-3-phosphate synthase [Amblyomma americanum].
ACCESSION   XP_077504478
VERSION     XP_077504478.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648352.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..139
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..139
                     /product="inositol-3-phosphate synthase"
                     /calculated_mol_wt=15715
     Region          3..>92
                     /region_name="NAD_binding_5"
                     /note="Myo-inositol-1-phosphate synthase; cl00554"
                     /db_xref="CDD:469819"
     CDS             1..139
                     /gene="Inos"
                     /coded_by="XM_077648352.1:178..597"
                     /db_xref="GeneID:144114548"
ORIGIN      
        1 mvkveidspt vrytadliea dyvystsdak vegnvcrvrp rdvrmtfrtk rrvprlgvml
       61 vgiggnngst vvaatlankl glswrtkdkv qvrarehprs afwrtkwvva adrrkngvvi
      121 givpycyryr pvrgvhert
//
LOCUS       XP_077504479             594 aa            linear   INV 25-NOV-2025
DEFINITION  NCK-interacting protein with SH3 domain isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504479
VERSION     XP_077504479.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648353.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..594
                     /product="NCK-interacting protein with SH3 domain isoform
                     X1"
                     /calculated_mol_wt=67346
     Region          3..59
                     /region_name="SH3"
                     /note="Src homology 3 domains; smart00326"
                     /db_xref="CDD:214620"
     Site            order(11,13,16,20,40..41,55,57..58)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212703"
     Region          435..559
                     /region_name="SPIN90_LRD"
                     /note="SPIN90/Ldb17, leucine-rich domain; pfam09431"
                     /db_xref="CDD:462796"
     CDS             1..594
                     /gene="LOC144114549"
                     /coded_by="XM_077648353.1:20..1804"
                     /db_xref="GeneID:144114549"
ORIGIN      
        1 masayqaksl ydfdskdkrl lsfkqdekfi vieqlckdpn wyyavsekgi agfvpvtyil
       61 reesereefl elvekaleal qnstssldeg ntsfmslqrk alrysnallr lhhlkeslrs
      121 tcegplrdtp retalghqsp sdflqeeysr insdwisenl agadlcslep sspaqaskrs
      181 asltaavdda hlptlaaeli eqvrsqtqls hslsrvavla vlrclstslp nsacpwegll
      241 dsvlkaeece kplvasqdgd rlqlifrrlw ncknddqqrs wpihedegli lglleelyti
      301 lvdadprvsr svvqadeydc vstlalyyqm epraslrvwm lrvfltlcel dlrvvslllh
      361 svlpmelard lqsntddier mkytallltv ifstgekppn nvyehvgerf vtflldties
      421 adveeevael slgtllslnl hlqsevdnfv lrtlatrkda ralterlmll vnreddparv
      481 lthevagvpn svlkmlaelf sdsttaelfy lndvavlidi varqlsdlpp gdkrrllyls
      541 lvehllgstq yeghrqaelr rcfesalthe essteekelv hriqltwpqw frmd
//
LOCUS       XP_077504480             588 aa            linear   INV 25-NOV-2025
DEFINITION  NCK-interacting protein with SH3 domain isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504480
VERSION     XP_077504480.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648354.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..588
                     /product="NCK-interacting protein with SH3 domain isoform
                     X2"
                     /calculated_mol_wt=66684
     Region          3..59
                     /region_name="SH3"
                     /note="Src homology 3 domains; smart00326"
                     /db_xref="CDD:214620"
     Site            order(11,13,16,20,40..41,55,57..58)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212690"
     Region          429..553
                     /region_name="SPIN90_LRD"
                     /note="SPIN90/Ldb17, leucine-rich domain; pfam09431"
                     /db_xref="CDD:462796"
     CDS             1..588
                     /gene="LOC144114549"
                     /coded_by="XM_077648354.1:10..1776"
                     /db_xref="GeneID:144114549"
ORIGIN      
        1 masayqaksl ydfdskdkrl lsfkqdekfi vieqlckdpn wyyavsekgi agfvpvtyil
       61 reesereefl elvekaleal qnstssldeg ntsfmslqrk alrrlhhlke slrstcegpl
      121 rdtpretalg hqspsdflqe eysrinsdwi senlagadlc slepsspaqa skrsasltaa
      181 vddahlptla aelieqvrsq tqlshslsrv avlavlrcls tslpnsacpw eglldsvlka
      241 eecekplvas qdgdrlqlif rrlwnckndd qqrswpihed eglilgllee lytilvdadp
      301 rvsrsvvqad eydcvstlal yyqmeprasl rvwmlrvflt lceldlrvvs lllhsvlpme
      361 lardlqsntd diermkytal lltvifstge kppnnvyehv gerfvtflld tiesadveee
      421 vaelslgtll slnlhlqsev dnfvlrtlat rkdaralter lmllvnredd parvltheva
      481 gvpnsvlkml aelfsdstta elfylndvav lidivarqls dlppgdkrrl lylslvehll
      541 gstqyeghrq aelrrcfesa ltheesstee kelvhriqlt wpqwfrmd
//
LOCUS       XP_077504481            1324 aa            linear   INV 25-NOV-2025
DEFINITION  patatin like phospholipase domain containing sws isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077504481
VERSION     XP_077504481.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648355.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1324
                     /product="patatin like phospholipase domain containing sws
                     isoform X1"
                     /calculated_mol_wt=147926
     Region          175..>312
                     /region_name="Crp"
                     /note="cAMP-binding domain of CRP or a regulatory subunit
                     of cAMP-dependent protein kinases [Signal transduction
                     mechanisms]; COG0664"
                     /db_xref="CDD:440428"
     Region          462..571
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(533..534,543..545)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Region          586..694
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(649..650,659..661)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Site            order(681..683,687..689)
                     /site_type="other"
                     /note="flexible hinge region"
                     /db_xref="CDD:237999"
     Region          907..1244
                     /region_name="Pat_PNPLA6_PNPLA7"
                     /note="Patatin-like phospholipase domain containing
                     protein 6 and protein 7; cd07225"
                     /db_xref="CDD:132864"
     Site            order(929..930,932,957,1077)
                     /site_type="active"
                     /db_xref="CDD:132864"
     Site            955..959
                     /site_type="other"
                     /note="nucleophile elbow"
                     /db_xref="CDD:132864"
     CDS             1..1324
                     /gene="sws"
                     /coded_by="XM_077648355.1:216..4190"
                     /db_xref="GeneID:144114550"
ORIGIN      
        1 msaflshfed fyfsgpywey wefivhssft almshmfehh lvtictvvll svavalgiil
       61 fyrkikskvd vvraqmlgss rprfrkrdkv lfygrkmlrk vnflttqats grtrlknrqm
      121 vlrfakrllr lkrdqpptlq vkepsqafle edyteqmekr lppdviymlr sirvfgffek
      181 plfmelwkhi etlfvprgtm lfslgdsdds iyvvqsgrih vhivepdgte ltfkevtage
      241 niasllsalv vltgvpstfk tvaaraleds silrlrydsf rilltkypds mirlcqivmi
      301 rlhrvtvtal hnylglttql mrsvrstggk kslalspkss pcrtpsrkls hvkeaavpva
      361 stagilgela vteeyghgsg petgsfhpsd datesekfas edpsgssfkt tssggrkvpa
      421 apettgsrks gkkasfvvge gsrtsssvqe yhdyleaavq gfmsrldied eemirnmvsv
      481 keyqpgeyls kedsgepasl vfilsgyvav sqktsakdsd dmlyiahvgd itgalevltg
      541 espiftrrak vlsrvaflts tqcyeilrmn ptavlfmars vierlspfvr qidfalewvy
      601 iesgravyrq dekaestaiv lsgrlrsvlt radgkremvg eygrgdlvgv velltgakra
      661 ttvmavrdte laklpeglln vikikypvvv trlihllghr llgslqkgdt vkaasmisrp
      721 tgsnfttiaa lavnedvpls tftlelchal rsigpvfrlt sdyikkqlga saldtaneyr
      781 lcnwlgqqed rnrvllyqcd sqmsswtqrc irqadciliv aladqeplvg qlekqaesia
      841 qrtqkelvll hregadkprn tvawlnmrsw csshhhircp krmfmrhsps riaevykkvq
      901 egvvnvhsdf arlarfltgt siglvlgggg argcahvgmi raiyeagvpi dmvggvsiga
      961 lvgalwcqev nvttlvqkgr hwskrmtspw sqlwdltypi tawftgaafn qtiydlfgdr
     1021 qiedlwlpyf tvttditssc mrihrhgslw rfirasmsls gymppmcdpv dghllldggy
     1081 vnnlpgslwh yvrasmsivg plpplcdpyd ghllvdgcyv nnvpadimre mgaetiiavd
     1141 vgsqddtdlt nygdclsgww llfkrwnpis ntvkipslpe iqsrlayvsc vrqleevkss
     1201 dyctyvrppi dryktlqfgs fdeimevgyv hgrtlfagmk agqrtlrsvl nldkarsfqa
     1261 gkegrpttft dlaemvcaik qpqkgrssvs dedeyecseq dfitdtekde epeepspgag
     1321 eval
//
LOCUS       XP_077504482            1322 aa            linear   INV 25-NOV-2025
DEFINITION  patatin like phospholipase domain containing sws isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077504482
VERSION     XP_077504482.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648356.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1322
                     /product="patatin like phospholipase domain containing sws
                     isoform X2"
                     /calculated_mol_wt=147670
     Region          175..>312
                     /region_name="Crp"
                     /note="cAMP-binding domain of CRP or a regulatory subunit
                     of cAMP-dependent protein kinases [Signal transduction
                     mechanisms]; COG0664"
                     /db_xref="CDD:440428"
     Region          460..569
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(531..532,541..543)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Region          584..692
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(647..648,657..659)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Site            order(679..681,685..687)
                     /site_type="other"
                     /note="flexible hinge region"
                     /db_xref="CDD:237999"
     Region          905..1242
                     /region_name="Pat_PNPLA6_PNPLA7"
                     /note="Patatin-like phospholipase domain containing
                     protein 6 and protein 7; cd07225"
                     /db_xref="CDD:132864"
     Site            order(927..928,930,955,1075)
                     /site_type="active"
                     /db_xref="CDD:132864"
     Site            953..957
                     /site_type="other"
                     /note="nucleophile elbow"
                     /db_xref="CDD:132864"
     CDS             1..1322
                     /gene="sws"
                     /coded_by="XM_077648356.1:224..4192"
                     /db_xref="GeneID:144114550"
ORIGIN      
        1 msaflshfed fyfsgpywey wefivhssft almshmfehh lvtictvvll svavalgiil
       61 fyrkikskvd vvraqmlgss rprfrkrdkv lfygrkmlrk vnflttqats grtrlknrqm
      121 vlrfakrllr lkrdqpptlq vkepsqafle edyteqmekr lppdviymlr sirvfgffek
      181 plfmelwkhi etlfvprgtm lfslgdsdds iyvvqsgrih vhivepdgte ltfkevtage
      241 niasllsalv vltgvpstfk tvaaraleds silrlrydsf rilltkypds mirlcqivmi
      301 rlhrvtvtal hnylglttql mrsstggkks lalspksspc rtpsrklshv keaavpvast
      361 agilgelavt eeyghgsgpe tgsfhpsdda tesekfased psgssfktts sggrkvpaap
      421 ettgsrksgk kasfvvgegs rtsssvqeyh dyleaavqgf msrldiedee mirnmvsvke
      481 yqpgeylske dsgepaslvf ilsgyvavsq ktsakdsddm lyiahvgdit galevltges
      541 piftrrakvl srvafltstq cyeilrmnpt avlfmarsvi erlspfvrqi dfalewvyie
      601 sgravyrqde kaestaivls grlrsvltra dgkremvgey grgdlvgvve lltgakratt
      661 vmavrdtela klpegllnvi kikypvvvtr lihllghrll gslqkgdtvk aasmisrptg
      721 snfttiaala vnedvplstf tlelchalrs igpvfrltsd yikkqlgasa ldtaneyrlc
      781 nwlgqqedrn rvllyqcdsq msswtqrcir qadcilival adqeplvgql ekqaesiaqr
      841 tqkelvllhr egadkprntv awlnmrswcs shhhircpkr mfmrhspsri aevykkvqeg
      901 vvnvhsdfar larfltgtsi glvlggggar gcahvgmira iyeagvpidm vggvsigalv
      961 galwcqevnv ttlvqkgrhw skrmtspwsq lwdltypita wftgaafnqt iydlfgdrqi
     1021 edlwlpyftv ttditsscmr ihrhgslwrf irasmslsgy mppmcdpvdg hllldggyvn
     1081 nlpgslwhyv rasmsivgpl pplcdpydgh llvdgcyvnn vpadimremg aetiiavdvg
     1141 sqddtdltny gdclsgwwll fkrwnpisnt vkipslpeiq srlayvscvr qleevkssdy
     1201 ctyvrppidr yktlqfgsfd eimevgyvhg rtlfagmkag qrtlrsvlnl dkarsfqagk
     1261 egrpttftdl aemvcaikqp qkgrssvsde deyecseqdf itdtekdeep eepspgagev
     1321 al
//
LOCUS       XP_077504483            1313 aa            linear   INV 25-NOV-2025
DEFINITION  patatin like phospholipase domain containing sws isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077504483
VERSION     XP_077504483.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648357.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1313
                     /product="patatin like phospholipase domain containing sws
                     isoform X3"
                     /calculated_mol_wt=146266
     Region          164..>301
                     /region_name="Crp"
                     /note="cAMP-binding domain of CRP or a regulatory subunit
                     of cAMP-dependent protein kinases [Signal transduction
                     mechanisms]; COG0664"
                     /db_xref="CDD:440428"
     Region          451..560
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(522..523,532..534)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Region          575..683
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(638..639,648..650)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Site            order(670..672,676..678)
                     /site_type="other"
                     /note="flexible hinge region"
                     /db_xref="CDD:237999"
     Region          896..1233
                     /region_name="Pat_PNPLA6_PNPLA7"
                     /note="Patatin-like phospholipase domain containing
                     protein 6 and protein 7; cd07225"
                     /db_xref="CDD:132864"
     Site            order(918..919,921,946,1066)
                     /site_type="active"
                     /db_xref="CDD:132864"
     Site            944..948
                     /site_type="other"
                     /note="nucleophile elbow"
                     /db_xref="CDD:132864"
     CDS             1..1313
                     /gene="sws"
                     /coded_by="XM_077648357.1:42..3983"
                     /db_xref="GeneID:144114550"
ORIGIN      
        1 mlteallfsl rtrsygllds linqarkpkp vtictvvlls vavalgiilf yrkikskvdv
       61 vraqmlgssr prfrkrdkvl fygrkmlrkv nflttqatsg rtrlknrqmv lrfakrllrl
      121 krdqpptlqv kepsqaflee dyteqmekrl ppdviymlrs irvfgffekp lfmelwkhie
      181 tlfvprgtml fslgdsddsi yvvqsgrihv hivepdgtel tfkevtagen iasllsalvv
      241 ltgvpstfkt vaaraledss ilrlrydsfr illtkypdsm irlcqivmir lhrvtvtalh
      301 nylglttqlm rsvrstggkk slalspkssp crtpsrklsh vkeaavpvas tagilgelav
      361 teeyghgsgp etgsfhpsdd atesekfase dpsgssfktt ssggrkvpaa pettgsrksg
      421 kkasfvvgeg srtsssvqey hdyleaavqg fmsrldiede emirnmvsvk eyqpgeylsk
      481 edsgepaslv filsgyvavs qktsakdsdd mlyiahvgdi tgalevltge spiftrrakv
      541 lsrvafltst qcyeilrmnp tavlfmarsv ierlspfvrq idfalewvyi esgravyrqd
      601 ekaestaivl sgrlrsvltr adgkremvge ygrgdlvgvv elltgakrat tvmavrdtel
      661 aklpegllnv ikikypvvvt rlihllghrl lgslqkgdtv kaasmisrpt gsnfttiaal
      721 avnedvplst ftlelchalr sigpvfrlts dyikkqlgas aldtaneyrl cnwlgqqedr
      781 nrvllyqcds qmsswtqrci rqadciliva ladqeplvgq lekqaesiaq rtqkelvllh
      841 regadkprnt vawlnmrswc sshhhircpk rmfmrhspsr iaevykkvqe gvvnvhsdfa
      901 rlarfltgts iglvlgggga rgcahvgmir aiyeagvpid mvggvsigal vgalwcqevn
      961 vttlvqkgrh wskrmtspws qlwdltypit awftgaafnq tiydlfgdrq iedlwlpyft
     1021 vttditsscm rihrhgslwr firasmslsg ymppmcdpvd ghllldggyv nnlpgslwhy
     1081 vrasmsivgp lpplcdpydg hllvdgcyvn nvpadimrem gaetiiavdv gsqddtdltn
     1141 ygdclsgwwl lfkrwnpisn tvkipslpei qsrlayvscv rqleevkssd yctyvrppid
     1201 ryktlqfgsf deimevgyvh grtlfagmka gqrtlrsvln ldkarsfqag kegrpttftd
     1261 laemvcaikq pqkgrssvsd edeyecseqd fitdtekdee peepspgage val
//
LOCUS       XP_077504484            1285 aa            linear   INV 25-NOV-2025
DEFINITION  patatin like phospholipase domain containing sws isoform X4
            [Amblyomma americanum].
ACCESSION   XP_077504484
VERSION     XP_077504484.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648358.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1285
                     /product="patatin like phospholipase domain containing sws
                     isoform X4"
                     /calculated_mol_wt=143688
     Region          175..>312
                     /region_name="Crp"
                     /note="cAMP-binding domain of CRP or a regulatory subunit
                     of cAMP-dependent protein kinases [Signal transduction
                     mechanisms]; COG0664"
                     /db_xref="CDD:440428"
     Region          462..571
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(533..534,543..545)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Region          586..694
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(649..650,659..661)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Site            order(681..683,687..689)
                     /site_type="other"
                     /note="flexible hinge region"
                     /db_xref="CDD:237999"
     Region          907..1205
                     /region_name="Pat_PNPLA6_PNPLA7"
                     /note="Patatin-like phospholipase domain containing
                     protein 6 and protein 7; cd07225"
                     /db_xref="CDD:132864"
     Site            order(929..930,932,957,1077)
                     /site_type="active"
                     /db_xref="CDD:132864"
     Site            955..959
                     /site_type="other"
                     /note="nucleophile elbow"
                     /db_xref="CDD:132864"
     CDS             1..1285
                     /gene="sws"
                     /coded_by="XM_077648358.1:414..4271"
                     /db_xref="GeneID:144114550"
ORIGIN      
        1 msaflshfed fyfsgpywey wefivhssft almshmfehh lvtictvvll svavalgiil
       61 fyrkikskvd vvraqmlgss rprfrkrdkv lfygrkmlrk vnflttqats grtrlknrqm
      121 vlrfakrllr lkrdqpptlq vkepsqafle edyteqmekr lppdviymlr sirvfgffek
      181 plfmelwkhi etlfvprgtm lfslgdsdds iyvvqsgrih vhivepdgte ltfkevtage
      241 niasllsalv vltgvpstfk tvaaraleds silrlrydsf rilltkypds mirlcqivmi
      301 rlhrvtvtal hnylglttql mrsvrstggk kslalspkss pcrtpsrkls hvkeaavpva
      361 stagilgela vteeyghgsg petgsfhpsd datesekfas edpsgssfkt tssggrkvpa
      421 apettgsrks gkkasfvvge gsrtsssvqe yhdyleaavq gfmsrldied eemirnmvsv
      481 keyqpgeyls kedsgepasl vfilsgyvav sqktsakdsd dmlyiahvgd itgalevltg
      541 espiftrrak vlsrvaflts tqcyeilrmn ptavlfmars vierlspfvr qidfalewvy
      601 iesgravyrq dekaestaiv lsgrlrsvlt radgkremvg eygrgdlvgv velltgakra
      661 ttvmavrdte laklpeglln vikikypvvv trlihllghr llgslqkgdt vkaasmisrp
      721 tgsnfttiaa lavnedvpls tftlelchal rsigpvfrlt sdyikkqlga saldtaneyr
      781 lcnwlgqqed rnrvllyqcd sqmsswtqrc irqadciliv aladqeplvg qlekqaesia
      841 qrtqkelvll hregadkprn tvawlnmrsw csshhhircp krmfmrhsps riaevykkvq
      901 egvvnvhsdf arlarfltgt siglvlgggg argcahvgmi raiyeagvpi dmvggvsiga
      961 lvgalwcqev nvttlvqkgr hwskrmtspw sqlwdltypi tawftgaafn qtiydlfgdr
     1021 qiedlwlpyf tvttditssc mrihrhgslw rfirasmsls gymppmcdpv dghllldggy
     1081 vnnlpadimr emgaetiiav dvgsqddtdl tnygdclsgw wllfkrwnpi sntvkipslp
     1141 eiqsrlayvs cvrqleevks sdyctyvrpp idryktlqfg sfdeimevgy vhgrtlfagm
     1201 kagqrtlrsv lnldkarsfq agkegrpttf tdlaemvcai kqpqkgrssv sdedeyecse
     1261 qdfitdtekd eepeepspga geval
//
LOCUS       XP_077504485            1285 aa            linear   INV 25-NOV-2025
DEFINITION  patatin like phospholipase domain containing sws isoform X5
            [Amblyomma americanum].
ACCESSION   XP_077504485
VERSION     XP_077504485.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648359.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1285
                     /product="patatin like phospholipase domain containing sws
                     isoform X5"
                     /calculated_mol_wt=143663
     Region          175..>312
                     /region_name="Crp"
                     /note="cAMP-binding domain of CRP or a regulatory subunit
                     of cAMP-dependent protein kinases [Signal transduction
                     mechanisms]; COG0664"
                     /db_xref="CDD:440428"
     Region          462..571
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(533..534,543..545)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Region          586..694
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(649..650,659..661)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Site            order(681..683,687..689)
                     /site_type="other"
                     /note="flexible hinge region"
                     /db_xref="CDD:237999"
     Region          907..1205
                     /region_name="Pat_PNPLA6_PNPLA7"
                     /note="Patatin-like phospholipase domain containing
                     protein 6 and protein 7; cd07225"
                     /db_xref="CDD:132864"
     Site            order(929..930,932,957,1077)
                     /site_type="active"
                     /db_xref="CDD:132864"
     Site            955..959
                     /site_type="other"
                     /note="nucleophile elbow"
                     /db_xref="CDD:132864"
     CDS             1..1285
                     /gene="sws"
                     /coded_by="XM_077648359.1:219..4076"
                     /db_xref="GeneID:144114550"
ORIGIN      
        1 msaflshfed fyfsgpywey wefivhssft almshmfehh lvtictvvll svavalgiil
       61 fyrkikskvd vvraqmlgss rprfrkrdkv lfygrkmlrk vnflttqats grtrlknrqm
      121 vlrfakrllr lkrdqpptlq vkepsqafle edyteqmekr lppdviymlr sirvfgffek
      181 plfmelwkhi etlfvprgtm lfslgdsdds iyvvqsgrih vhivepdgte ltfkevtage
      241 niasllsalv vltgvpstfk tvaaraleds silrlrydsf rilltkypds mirlcqivmi
      301 rlhrvtvtal hnylglttql mrsvrstggk kslalspkss pcrtpsrkls hvkeaavpva
      361 stagilgela vteeyghgsg petgsfhpsd datesekfas edpsgssfkt tssggrkvpa
      421 apettgsrks gkkasfvvge gsrtsssvqe yhdyleaavq gfmsrldied eemirnmvsv
      481 keyqpgeyls kedsgepasl vfilsgyvav sqktsakdsd dmlyiahvgd itgalevltg
      541 espiftrrak vlsrvaflts tqcyeilrmn ptavlfmars vierlspfvr qidfalewvy
      601 iesgravyrq dekaestaiv lsgrlrsvlt radgkremvg eygrgdlvgv velltgakra
      661 ttvmavrdte laklpeglln vikikypvvv trlihllghr llgslqkgdt vkaasmisrp
      721 tgsnfttiaa lavnedvpls tftlelchal rsigpvfrlt sdyikkqlga saldtaneyr
      781 lcnwlgqqed rnrvllyqcd sqmsswtqrc irqadciliv aladqeplvg qlekqaesia
      841 qrtqkelvll hregadkprn tvawlnmrsw csshhhircp krmfmrhsps riaevykkvq
      901 egvvnvhsdf arlarfltgt siglvlgggg argcahvgmi raiyeagvpi dmvggvsiga
      961 lvgalwcqev nvttlvqkgr hwskrmtspw sqlwdltypi tawftgaafn qtiydlfgdr
     1021 qiedlwlpyf tvttditssc mrihrhgslw hyvrasmsiv gplpplcdpy dghllvdgcy
     1081 vnnvpadimr emgaetiiav dvgsqddtdl tnygdclsgw wllfkrwnpi sntvkipslp
     1141 eiqsrlayvs cvrqleevks sdyctyvrpp idryktlqfg sfdeimevgy vhgrtlfagm
     1201 kagqrtlrsv lnldkarsfq agkegrpttf tdlaemvcai kqpqkgrssv sdedeyecse
     1261 qdfitdtekd eepeepspga geval
//
LOCUS       XP_077504488            1283 aa            linear   INV 25-NOV-2025
DEFINITION  patatin like phospholipase domain containing sws isoform X6
            [Amblyomma americanum].
ACCESSION   XP_077504488
VERSION     XP_077504488.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648362.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1283
                     /product="patatin like phospholipase domain containing sws
                     isoform X6"
                     /calculated_mol_wt=143432
     Region          175..>312
                     /region_name="Crp"
                     /note="cAMP-binding domain of CRP or a regulatory subunit
                     of cAMP-dependent protein kinases [Signal transduction
                     mechanisms]; COG0664"
                     /db_xref="CDD:440428"
     Region          460..569
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(531..532,541..543)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Region          584..692
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(647..648,657..659)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Site            order(679..681,685..687)
                     /site_type="other"
                     /note="flexible hinge region"
                     /db_xref="CDD:237999"
     Region          905..1203
                     /region_name="Pat_PNPLA6_PNPLA7"
                     /note="Patatin-like phospholipase domain containing
                     protein 6 and protein 7; cd07225"
                     /db_xref="CDD:132864"
     Site            order(927..928,930,955,1075)
                     /site_type="active"
                     /db_xref="CDD:132864"
     Site            953..957
                     /site_type="other"
                     /note="nucleophile elbow"
                     /db_xref="CDD:132864"
     CDS             1..1283
                     /gene="sws"
                     /coded_by="XM_077648362.1:224..4075"
                     /db_xref="GeneID:144114550"
ORIGIN      
        1 msaflshfed fyfsgpywey wefivhssft almshmfehh lvtictvvll svavalgiil
       61 fyrkikskvd vvraqmlgss rprfrkrdkv lfygrkmlrk vnflttqats grtrlknrqm
      121 vlrfakrllr lkrdqpptlq vkepsqafle edyteqmekr lppdviymlr sirvfgffek
      181 plfmelwkhi etlfvprgtm lfslgdsdds iyvvqsgrih vhivepdgte ltfkevtage
      241 niasllsalv vltgvpstfk tvaaraleds silrlrydsf rilltkypds mirlcqivmi
      301 rlhrvtvtal hnylglttql mrsstggkks lalspksspc rtpsrklshv keaavpvast
      361 agilgelavt eeyghgsgpe tgsfhpsdda tesekfased psgssfktts sggrkvpaap
      421 ettgsrksgk kasfvvgegs rtsssvqeyh dyleaavqgf msrldiedee mirnmvsvke
      481 yqpgeylske dsgepaslvf ilsgyvavsq ktsakdsddm lyiahvgdit galevltges
      541 piftrrakvl srvafltstq cyeilrmnpt avlfmarsvi erlspfvrqi dfalewvyie
      601 sgravyrqde kaestaivls grlrsvltra dgkremvgey grgdlvgvve lltgakratt
      661 vmavrdtela klpegllnvi kikypvvvtr lihllghrll gslqkgdtvk aasmisrptg
      721 snfttiaala vnedvplstf tlelchalrs igpvfrltsd yikkqlgasa ldtaneyrlc
      781 nwlgqqedrn rvllyqcdsq msswtqrcir qadcilival adqeplvgql ekqaesiaqr
      841 tqkelvllhr egadkprntv awlnmrswcs shhhircpkr mfmrhspsri aevykkvqeg
      901 vvnvhsdfar larfltgtsi glvlggggar gcahvgmira iyeagvpidm vggvsigalv
      961 galwcqevnv ttlvqkgrhw skrmtspwsq lwdltypita wftgaafnqt iydlfgdrqi
     1021 edlwlpyftv ttditsscmr ihrhgslwrf irasmslsgy mppmcdpvdg hllldggyvn
     1081 nlpadimrem gaetiiavdv gsqddtdltn ygdclsgwwl lfkrwnpisn tvkipslpei
     1141 qsrlayvscv rqleevkssd yctyvrppid ryktlqfgsf deimevgyvh grtlfagmka
     1201 gqrtlrsvln ldkarsfqag kegrpttftd laemvcaikq pqkgrssvsd edeyecseqd
     1261 fitdtekdee peepspgage val
//
LOCUS       XP_077504489            1283 aa            linear   INV 25-NOV-2025
DEFINITION  patatin like phospholipase domain containing sws isoform X7
            [Amblyomma americanum].
ACCESSION   XP_077504489
VERSION     XP_077504489.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648363.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1283
                     /product="patatin like phospholipase domain containing sws
                     isoform X7"
                     /calculated_mol_wt=143407
     Region          175..>312
                     /region_name="Crp"
                     /note="cAMP-binding domain of CRP or a regulatory subunit
                     of cAMP-dependent protein kinases [Signal transduction
                     mechanisms]; COG0664"
                     /db_xref="CDD:440428"
     Region          460..569
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(531..532,541..543)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Region          584..692
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(647..648,657..659)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Site            order(679..681,685..687)
                     /site_type="other"
                     /note="flexible hinge region"
                     /db_xref="CDD:237999"
     Region          905..1203
                     /region_name="Pat_PNPLA6_PNPLA7"
                     /note="Patatin-like phospholipase domain containing
                     protein 6 and protein 7; cd07225"
                     /db_xref="CDD:132864"
     Site            order(927..928,930,955,1075)
                     /site_type="active"
                     /db_xref="CDD:132864"
     Site            953..957
                     /site_type="other"
                     /note="nucleophile elbow"
                     /db_xref="CDD:132864"
     CDS             1..1283
                     /gene="sws"
                     /coded_by="XM_077648363.1:224..4075"
                     /db_xref="GeneID:144114550"
ORIGIN      
        1 msaflshfed fyfsgpywey wefivhssft almshmfehh lvtictvvll svavalgiil
       61 fyrkikskvd vvraqmlgss rprfrkrdkv lfygrkmlrk vnflttqats grtrlknrqm
      121 vlrfakrllr lkrdqpptlq vkepsqafle edyteqmekr lppdviymlr sirvfgffek
      181 plfmelwkhi etlfvprgtm lfslgdsdds iyvvqsgrih vhivepdgte ltfkevtage
      241 niasllsalv vltgvpstfk tvaaraleds silrlrydsf rilltkypds mirlcqivmi
      301 rlhrvtvtal hnylglttql mrsstggkks lalspksspc rtpsrklshv keaavpvast
      361 agilgelavt eeyghgsgpe tgsfhpsdda tesekfased psgssfktts sggrkvpaap
      421 ettgsrksgk kasfvvgegs rtsssvqeyh dyleaavqgf msrldiedee mirnmvsvke
      481 yqpgeylske dsgepaslvf ilsgyvavsq ktsakdsddm lyiahvgdit galevltges
      541 piftrrakvl srvafltstq cyeilrmnpt avlfmarsvi erlspfvrqi dfalewvyie
      601 sgravyrqde kaestaivls grlrsvltra dgkremvgey grgdlvgvve lltgakratt
      661 vmavrdtela klpegllnvi kikypvvvtr lihllghrll gslqkgdtvk aasmisrptg
      721 snfttiaala vnedvplstf tlelchalrs igpvfrltsd yikkqlgasa ldtaneyrlc
      781 nwlgqqedrn rvllyqcdsq msswtqrcir qadcilival adqeplvgql ekqaesiaqr
      841 tqkelvllhr egadkprntv awlnmrswcs shhhircpkr mfmrhspsri aevykkvqeg
      901 vvnvhsdfar larfltgtsi glvlggggar gcahvgmira iyeagvpidm vggvsigalv
      961 galwcqevnv ttlvqkgrhw skrmtspwsq lwdltypita wftgaafnqt iydlfgdrqi
     1021 edlwlpyftv ttditsscmr ihrhgslwhy vrasmsivgp lpplcdpydg hllvdgcyvn
     1081 nvpadimrem gaetiiavdv gsqddtdltn ygdclsgwwl lfkrwnpisn tvkipslpei
     1141 qsrlayvscv rqleevkssd yctyvrppid ryktlqfgsf deimevgyvh grtlfagmka
     1201 gqrtlrsvln ldkarsfqag kegrpttftd laemvcaikq pqkgrssvsd edeyecseqd
     1261 fitdtekdee peepspgage val
//
LOCUS       XP_077504490            1274 aa            linear   INV 25-NOV-2025
DEFINITION  patatin like phospholipase domain containing sws isoform X8
            [Amblyomma americanum].
ACCESSION   XP_077504490
VERSION     XP_077504490.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648364.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1274
                     /product="patatin like phospholipase domain containing sws
                     isoform X8"
                     /calculated_mol_wt=142028
     Region          164..>301
                     /region_name="Crp"
                     /note="cAMP-binding domain of CRP or a regulatory subunit
                     of cAMP-dependent protein kinases [Signal transduction
                     mechanisms]; COG0664"
                     /db_xref="CDD:440428"
     Region          451..560
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(522..523,532..534)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Region          575..683
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(638..639,648..650)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Site            order(670..672,676..678)
                     /site_type="other"
                     /note="flexible hinge region"
                     /db_xref="CDD:237999"
     Region          896..1194
                     /region_name="Pat_PNPLA6_PNPLA7"
                     /note="Patatin-like phospholipase domain containing
                     protein 6 and protein 7; cd07225"
                     /db_xref="CDD:132864"
     Site            order(918..919,921,946,1066)
                     /site_type="active"
                     /db_xref="CDD:132864"
     Site            944..948
                     /site_type="other"
                     /note="nucleophile elbow"
                     /db_xref="CDD:132864"
     CDS             1..1274
                     /gene="sws"
                     /coded_by="XM_077648364.1:39..3863"
                     /db_xref="GeneID:144114550"
ORIGIN      
        1 mlteallfsl rtrsygllds linqarkpkp vtictvvlls vavalgiilf yrkikskvdv
       61 vraqmlgssr prfrkrdkvl fygrkmlrkv nflttqatsg rtrlknrqmv lrfakrllrl
      121 krdqpptlqv kepsqaflee dyteqmekrl ppdviymlrs irvfgffekp lfmelwkhie
      181 tlfvprgtml fslgdsddsi yvvqsgrihv hivepdgtel tfkevtagen iasllsalvv
      241 ltgvpstfkt vaaraledss ilrlrydsfr illtkypdsm irlcqivmir lhrvtvtalh
      301 nylglttqlm rsvrstggkk slalspkssp crtpsrklsh vkeaavpvas tagilgelav
      361 teeyghgsgp etgsfhpsdd atesekfase dpsgssfktt ssggrkvpaa pettgsrksg
      421 kkasfvvgeg srtsssvqey hdyleaavqg fmsrldiede emirnmvsvk eyqpgeylsk
      481 edsgepaslv filsgyvavs qktsakdsdd mlyiahvgdi tgalevltge spiftrrakv
      541 lsrvafltst qcyeilrmnp tavlfmarsv ierlspfvrq idfalewvyi esgravyrqd
      601 ekaestaivl sgrlrsvltr adgkremvge ygrgdlvgvv elltgakrat tvmavrdtel
      661 aklpegllnv ikikypvvvt rlihllghrl lgslqkgdtv kaasmisrpt gsnfttiaal
      721 avnedvplst ftlelchalr sigpvfrlts dyikkqlgas aldtaneyrl cnwlgqqedr
      781 nrvllyqcds qmsswtqrci rqadciliva ladqeplvgq lekqaesiaq rtqkelvllh
      841 regadkprnt vawlnmrswc sshhhircpk rmfmrhspsr iaevykkvqe gvvnvhsdfa
      901 rlarfltgts iglvlgggga rgcahvgmir aiyeagvpid mvggvsigal vgalwcqevn
      961 vttlvqkgrh wskrmtspws qlwdltypit awftgaafnq tiydlfgdrq iedlwlpyft
     1021 vttditsscm rihrhgslwr firasmslsg ymppmcdpvd ghllldggyv nnlpadimre
     1081 mgaetiiavd vgsqddtdlt nygdclsgww llfkrwnpis ntvkipslpe iqsrlayvsc
     1141 vrqleevkss dyctyvrppi dryktlqfgs fdeimevgyv hgrtlfagmk agqrtlrsvl
     1201 nldkarsfqa gkegrpttft dlaemvcaik qpqkgrssvs dedeyecseq dfitdtekde
     1261 epeepspgag eval
//
LOCUS       XP_077504491            1272 aa            linear   INV 25-NOV-2025
DEFINITION  patatin like phospholipase domain containing sws isoform X9
            [Amblyomma americanum].
ACCESSION   XP_077504491
VERSION     XP_077504491.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648365.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1272
                     /product="patatin like phospholipase domain containing sws
                     isoform X9"
                     /calculated_mol_wt=141773
     Region          164..>301
                     /region_name="Crp"
                     /note="cAMP-binding domain of CRP or a regulatory subunit
                     of cAMP-dependent protein kinases [Signal transduction
                     mechanisms]; COG0664"
                     /db_xref="CDD:440428"
     Region          449..558
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(520..521,530..532)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Region          573..681
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(636..637,646..648)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Site            order(668..670,674..676)
                     /site_type="other"
                     /note="flexible hinge region"
                     /db_xref="CDD:237999"
     Region          894..1192
                     /region_name="Pat_PNPLA6_PNPLA7"
                     /note="Patatin-like phospholipase domain containing
                     protein 6 and protein 7; cd07225"
                     /db_xref="CDD:132864"
     Site            order(916..917,919,944,1064)
                     /site_type="active"
                     /db_xref="CDD:132864"
     Site            942..946
                     /site_type="other"
                     /note="nucleophile elbow"
                     /db_xref="CDD:132864"
     CDS             1..1272
                     /gene="sws"
                     /coded_by="XM_077648365.1:38..3856"
                     /db_xref="GeneID:144114550"
ORIGIN      
        1 mlteallfsl rtrsygllds linqarkpkp vtictvvlls vavalgiilf yrkikskvdv
       61 vraqmlgssr prfrkrdkvl fygrkmlrkv nflttqatsg rtrlknrqmv lrfakrllrl
      121 krdqpptlqv kepsqaflee dyteqmekrl ppdviymlrs irvfgffekp lfmelwkhie
      181 tlfvprgtml fslgdsddsi yvvqsgrihv hivepdgtel tfkevtagen iasllsalvv
      241 ltgvpstfkt vaaraledss ilrlrydsfr illtkypdsm irlcqivmir lhrvtvtalh
      301 nylglttqlm rsstggkksl alspksspcr tpsrklshvk eaavpvasta gilgelavte
      361 eyghgsgpet gsfhpsddat esekfasedp sgssfkttss ggrkvpaape ttgsrksgkk
      421 asfvvgegsr tsssvqeyhd yleaavqgfm srldiedeem irnmvsvkey qpgeylsked
      481 sgepaslvfi lsgyvavsqk tsakdsddml yiahvgditg alevltgesp iftrrakvls
      541 rvafltstqc yeilrmnpta vlfmarsvie rlspfvrqid falewvyies gravyrqdek
      601 aestaivlsg rlrsvltrad gkremvgeyg rgdlvgvvel ltgakrattv mavrdtelak
      661 lpegllnvik ikypvvvtrl ihllghrllg slqkgdtvka asmisrptgs nfttiaalav
      721 nedvplstft lelchalrsi gpvfrltsdy ikkqlgasal dtaneyrlcn wlgqqedrnr
      781 vllyqcdsqm sswtqrcirq adcilivala dqeplvgqle kqaesiaqrt qkelvllhre
      841 gadkprntva wlnmrswcss hhhircpkrm fmrhspsria evykkvqegv vnvhsdfarl
      901 arfltgtsig lvlggggarg cahvgmirai yeagvpidmv ggvsigalvg alwcqevnvt
      961 tlvqkgrhws krmtspwsql wdltypitaw ftgaafnqti ydlfgdrqie dlwlpyftvt
     1021 tditsscmri hrhgslwrfi rasmslsgym ppmcdpvdgh llldggyvnn lpadimremg
     1081 aetiiavdvg sqddtdltny gdclsgwwll fkrwnpisnt vkipslpeiq srlayvscvr
     1141 qleevkssdy ctyvrppidr yktlqfgsfd eimevgyvhg rtlfagmkag qrtlrsvlnl
     1201 dkarsfqagk egrpttftdl aemvcaikqp qkgrssvsde deyecseqdf itdtekdeep
     1261 eepspgagev al
//
LOCUS       XP_077504492            1168 aa            linear   INV 25-NOV-2025
DEFINITION  patatin like phospholipase domain containing sws isoform X10
            [Amblyomma americanum].
ACCESSION   XP_077504492
VERSION     XP_077504492.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648366.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1168
                     /product="patatin like phospholipase domain containing sws
                     isoform X10"
                     /calculated_mol_wt=129413
     Region          19..>156
                     /region_name="Crp"
                     /note="cAMP-binding domain of CRP or a regulatory subunit
                     of cAMP-dependent protein kinases [Signal transduction
                     mechanisms]; COG0664"
                     /db_xref="CDD:440428"
     Region          306..415
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(377..378,387..389)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Region          430..538
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(493..494,503..505)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Site            order(525..527,531..533)
                     /site_type="other"
                     /note="flexible hinge region"
                     /db_xref="CDD:237999"
     Region          751..1088
                     /region_name="Pat_PNPLA6_PNPLA7"
                     /note="Patatin-like phospholipase domain containing
                     protein 6 and protein 7; cd07225"
                     /db_xref="CDD:132864"
     Site            order(773..774,776,801,921)
                     /site_type="active"
                     /db_xref="CDD:132864"
     Site            799..803
                     /site_type="other"
                     /note="nucleophile elbow"
                     /db_xref="CDD:132864"
     CDS             1..1168
                     /gene="sws"
                     /coded_by="XM_077648366.1:105..3611"
                     /db_xref="GeneID:144114550"
ORIGIN      
        1 mekrlppdvi ymlrsirvfg ffekplfmel wkhietlfvp rgtmlfslgd sddsiyvvqs
       61 grihvhivep dgteltfkev tageniasll salvvltgvp stfktvaara ledssilrlr
      121 ydsfrilltk ypdsmirlcq ivmirlhrvt vtalhnylgl ttqlmrsvrs tggkkslals
      181 pksspcrtps rklshvkeaa vpvastagil gelavteeyg hgsgpetgsf hpsddatese
      241 kfasedpsgs sfkttssggr kvpaapettg srksgkkasf vvgegsrtss svqeyhdyle
      301 aavqgfmsrl diedeemirn mvsvkeyqpg eylskedsge paslvfilsg yvavsqktsa
      361 kdsddmlyia hvgditgale vltgespift rrakvlsrva fltstqcyei lrmnptavlf
      421 marsvierls pfvrqidfal ewvyiesgra vyrqdekaes taivlsgrlr svltradgkr
      481 emvgeygrgd lvgvvelltg akrattvmav rdtelaklpe gllnvikiky pvvvtrlihl
      541 lghrllgslq kgdtvkaasm isrptgsnft tiaalavned vplstftlel chalrsigpv
      601 frltsdyikk qlgasaldta neyrlcnwlg qqedrnrvll yqcdsqmssw tqrcirqadc
      661 ilivaladqe plvgqlekqa esiaqrtqke lvllhregad kprntvawln mrswcsshhh
      721 ircpkrmfmr hspsriaevy kkvqegvvnv hsdfarlarf ltgtsiglvl ggggargcah
      781 vgmiraiyea gvpidmvggv sigalvgalw cqevnvttlv qkgrhwskrm tspwsqlwdl
      841 typitawftg aafnqtiydl fgdrqiedlw lpyftvttdi tsscmrihrh gslwrfiras
      901 mslsgymppm cdpvdghlll dggyvnnlpg slwhyvrasm sivgplpplc dpydghllvd
      961 gcyvnnvpad imremgaeti iavdvgsqdd tdltnygdcl sgwwllfkrw npisntvkip
     1021 slpeiqsrla yvscvrqlee vkssdyctyv rppidryktl qfgsfdeime vgyvhgrtlf
     1081 agmkagqrtl rsvlnldkar sfqagkegrp ttftdlaemv caikqpqkgr ssvsdedeye
     1141 cseqdfitdt ekdeepeeps pgageval
//
LOCUS       XP_077504493            1096 aa            linear   INV 25-NOV-2025
DEFINITION  patatin like phospholipase domain containing sws isoform X11
            [Amblyomma americanum].
ACCESSION   XP_077504493
VERSION     XP_077504493.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648367.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1096
                     /product="patatin like phospholipase domain containing sws
                     isoform X11"
                     /calculated_mol_wt=122564
     Region          175..>312
                     /region_name="Crp"
                     /note="cAMP-binding domain of CRP or a regulatory subunit
                     of cAMP-dependent protein kinases [Signal transduction
                     mechanisms]; COG0664"
                     /db_xref="CDD:440428"
     Region          462..571
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(533..534,543..545)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Region          586..694
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(649..650,659..661)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Site            order(681..683,687..689)
                     /site_type="other"
                     /note="flexible hinge region"
                     /db_xref="CDD:237999"
     Region          907..>1085
                     /region_name="Patatin_and_cPLA2"
                     /note="Patatins and Phospholipases; cl11396"
                     /db_xref="CDD:416256"
     Site            order(929..930,932,957,1077)
                     /site_type="active"
                     /db_xref="CDD:132836"
     Site            955..959
                     /site_type="other"
                     /note="nucleophile elbow"
                     /db_xref="CDD:132836"
     CDS             1..1096
                     /gene="sws"
                     /coded_by="XM_077648367.1:216..3506"
                     /db_xref="GeneID:144114550"
ORIGIN      
        1 msaflshfed fyfsgpywey wefivhssft almshmfehh lvtictvvll svavalgiil
       61 fyrkikskvd vvraqmlgss rprfrkrdkv lfygrkmlrk vnflttqats grtrlknrqm
      121 vlrfakrllr lkrdqpptlq vkepsqafle edyteqmekr lppdviymlr sirvfgffek
      181 plfmelwkhi etlfvprgtm lfslgdsdds iyvvqsgrih vhivepdgte ltfkevtage
      241 niasllsalv vltgvpstfk tvaaraleds silrlrydsf rilltkypds mirlcqivmi
      301 rlhrvtvtal hnylglttql mrsvrstggk kslalspkss pcrtpsrkls hvkeaavpva
      361 stagilgela vteeyghgsg petgsfhpsd datesekfas edpsgssfkt tssggrkvpa
      421 apettgsrks gkkasfvvge gsrtsssvqe yhdyleaavq gfmsrldied eemirnmvsv
      481 keyqpgeyls kedsgepasl vfilsgyvav sqktsakdsd dmlyiahvgd itgalevltg
      541 espiftrrak vlsrvaflts tqcyeilrmn ptavlfmars vierlspfvr qidfalewvy
      601 iesgravyrq dekaestaiv lsgrlrsvlt radgkremvg eygrgdlvgv velltgakra
      661 ttvmavrdte laklpeglln vikikypvvv trlihllghr llgslqkgdt vkaasmisrp
      721 tgsnfttiaa lavnedvpls tftlelchal rsigpvfrlt sdyikkqlga saldtaneyr
      781 lcnwlgqqed rnrvllyqcd sqmsswtqrc irqadciliv aladqeplvg qlekqaesia
      841 qrtqkelvll hregadkprn tvawlnmrsw csshhhircp krmfmrhsps riaevykkvq
      901 egvvnvhsdf arlarfltgt siglvlgggg argcahvgmi raiyeagvpi dmvggvsiga
      961 lvgalwcqev nvttlvqkgr hwskrmtspw sqlwdltypi tawftgaafn qtiydlfgdr
     1021 qiedlwlpyf tvttditssc mrihrhgslw rfirasmsls gymppmcdpv dghllldggy
     1081 vnnlpewccc efgpqk
//
LOCUS       XP_077504494             640 aa            linear   INV 25-NOV-2025
DEFINITION  folylpolyglutamate synthase, mitochondrial-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077504494
VERSION     XP_077504494.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648368.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..640
                     /product="folylpolyglutamate synthase, mitochondrial-like
                     isoform X1"
                     /calculated_mol_wt=71311
     Region          19..521
                     /region_name="PLN02881"
                     /note="tetrahydrofolylpolyglutamate synthase; cl47075"
                     /db_xref="CDD:481415"
     CDS             1..640
                     /gene="LOC144114552"
                     /coded_by="XM_077648368.1:340..2262"
                     /db_xref="GeneID:144114552"
ORIGIN      
        1 mrnllwrlkm vkptqvrtvg diptkpmptv sskrcyedav yalnnlqsne atlkksmqhp
       61 staflrsrrm pehlqaiglq isdidalkii hvagtkgkgs tcafaesilr hhgfktglyt
      121 sphlvavrer iringkplse dlfakyfwdv yndlkikega nmpayfhflt vmafkvfvke
      181 kvdvlvlevg vggeydctnv irhptvvgit slgldhtrll gssvqeiawq kggifkkgcp
      241 afslpqpega fkvlqerane rgcslsiapp lltyecevpl crlgipgepq qgnaslalql
      301 cktwlakhcs tdsalgkkqa erdtiarkwl ehhdlpfiaa pfpisklmak glgqcrwpgr
      361 cqvvpagdgm tlyldgahte dsvhycvrww rdasaaeqha lgprvqvrrv llfncvgdrr
      421 peillsylae epfhvalftp nrlsntkspy sdqsdftvek ctetarcean mriwchllss
      481 lqeeeilgsg cptsppslrg npedsclvfs sisntmswlq eqqvaarklt ppchiqktss
      541 artreyelra lgypaevprf epgrggrvsm dvkckgvgnr epsfdwwsdv ltgsrtctav
      601 taamqylptc ilwtahfvil kqtkksgiws qncsrasqil
//
LOCUS       XP_077504495             584 aa            linear   INV 25-NOV-2025
DEFINITION  folylpolyglutamate synthase, mitochondrial-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077504495
VERSION     XP_077504495.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648369.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..584
                     /product="folylpolyglutamate synthase, mitochondrial-like
                     isoform X2"
                     /calculated_mol_wt=64906
     Region          12..465
                     /region_name="PLN02881"
                     /note="tetrahydrofolylpolyglutamate synthase; cl47075"
                     /db_xref="CDD:481415"
     CDS             1..584
                     /gene="LOC144114552"
                     /coded_by="XM_077648369.1:261..2015"
                     /db_xref="GeneID:144114552"
ORIGIN      
        1 mqhpstaflr srrmpehlqa iglqisdida lkiihvagtk gkgstcafae silrhhgfkt
       61 glytsphlva vreriringk plsedlfaky fwdvyndlki keganmpayf hfltvmafkv
      121 fvkekvdvlv levgvggeyd ctnvirhptv vgitslgldh trllgssvqe iawqkggifk
      181 kgcpafslpq pegafkvlqe ranergcsls iapplltyec evplcrlgip gepqqgnasl
      241 alqlcktwla khcstdsalg kkqaerdtia rkwlehhdlp fiaapfpisk lmakglgqcr
      301 wpgrcqvvpa gdgmtlyldg ahtedsvhyc vrwwrdasaa eqhalgprvq vrrvllfncv
      361 gdrrpeills ylaeepfhva lftpnrlsnt kspysdqsdf tvekctetar ceanmriwch
      421 llsslqeeei lgsgcptspp slrgnpedsc lvfssisntm swlqeqqvaa rkltppchiq
      481 ktssartrey elralgypae vprfepgrgg rvsmdvkckg vgnrepsfdw wsdvltgsrt
      541 ctavtaamqy lptcilwtah fvilkqtkks giwsqncsra sqil
//
LOCUS       XP_077504496             560 aa            linear   INV 25-NOV-2025
DEFINITION  folylpolyglutamate synthase, mitochondrial-like isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077504496
VERSION     XP_077504496.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648370.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..560
                     /product="folylpolyglutamate synthase, mitochondrial-like
                     isoform X3"
                     /calculated_mol_wt=62101
     Region          19..553
                     /region_name="PLN02881"
                     /note="tetrahydrofolylpolyglutamate synthase; cl47075"
                     /db_xref="CDD:481415"
     CDS             1..560
                     /gene="LOC144114552"
                     /coded_by="XM_077648370.1:349..2031"
                     /db_xref="GeneID:144114552"
ORIGIN      
        1 mrnllwrlkm vkptqvrtvg diptkpmptv sskrcyedav yalnnlqsne atlkksmqhp
       61 staflrsrrm pehlqaiglq isdidalkii hvagtkgkgs tcafaesilr hhgfktglyt
      121 sphlvavrer iringkplse dlfakyfwdv yndlkikega nmpayfhflt vmafkvfvke
      181 kvdvlvlevg vggeydctnv irhptvvgit slgldhtrll gssvqeiawq kggifkkgcp
      241 afslpqpega fkvlqerane rgcslsiapp lltyecevpl crlgipgepq qgnaslalql
      301 cktwlakhcs tdsalgkkqa erdtiarkwl ehhdlpfiaa pfpisklmak glgqcrwpgr
      361 cqvvpagdgm tlyldgahte dsvhycvrww rdasaaeqha lgprvqvrrv llfncvgdrr
      421 peillsylae epfhvalftp nrlsntkspy sdqsdftvek ctetarcean mriwchllss
      481 lqeeeilgsg cptsppslrg npedsclvfs sisntmswlq eqqvaarklt ppchiqvlat
      541 gslhliggvm slldpelall
//
LOCUS       XP_077504497             504 aa            linear   INV 25-NOV-2025
DEFINITION  folylpolyglutamate synthase, mitochondrial-like isoform X4
            [Amblyomma americanum].
ACCESSION   XP_077504497
VERSION     XP_077504497.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648371.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..504
                     /product="folylpolyglutamate synthase, mitochondrial-like
                     isoform X4"
                     /calculated_mol_wt=55697
     Region          12..497
                     /region_name="PLN02881"
                     /note="tetrahydrofolylpolyglutamate synthase; cl47075"
                     /db_xref="CDD:481415"
     CDS             1..504
                     /gene="LOC144114552"
                     /coded_by="XM_077648371.1:580..2094"
                     /db_xref="GeneID:144114552"
ORIGIN      
        1 mqhpstaflr srrmpehlqa iglqisdida lkiihvagtk gkgstcafae silrhhgfkt
       61 glytsphlva vreriringk plsedlfaky fwdvyndlki keganmpayf hfltvmafkv
      121 fvkekvdvlv levgvggeyd ctnvirhptv vgitslgldh trllgssvqe iawqkggifk
      181 kgcpafslpq pegafkvlqe ranergcsls iapplltyec evplcrlgip gepqqgnasl
      241 alqlcktwla khcstdsalg kkqaerdtia rkwlehhdlp fiaapfpisk lmakglgqcr
      301 wpgrcqvvpa gdgmtlyldg ahtedsvhyc vrwwrdasaa eqhalgprvq vrrvllfncv
      361 gdrrpeills ylaeepfhva lftpnrlsnt kspysdqsdf tvekctetar ceanmriwch
      421 llsslqeeei lgsgcptspp slrgnpedsc lvfssisntm swlqeqqvaa rkltppchiq
      481 vlatgslhli ggvmslldpe lall
//
LOCUS       XP_077504498             420 aa            linear   INV 25-NOV-2025
DEFINITION  histone-lysine N-methyltransferase SMYD3-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504498
VERSION     XP_077504498.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648372.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..420
                     /product="histone-lysine N-methyltransferase SMYD3-like
                     isoform X1"
                     /calculated_mol_wt=47891
     Region          5..250
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(183..188,196..198,221..224)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Region          308..381
                     /region_name="TPR_12"
                     /note="Tetratricopeptide repeat; pfam13424"
                     /db_xref="CDD:315987"
     CDS             1..420
                     /gene="LOC144114554"
                     /coded_by="XM_077648372.1:31..1293"
                     /db_xref="GeneID:144114554"
ORIGIN      
        1 mltykagdei lsstpfvyvl gresrgrvcd ycfsecgnlk rcaackcvyy cnkpcqrqaw
       61 qdhkvecaal ckvapnvpet lvryfckllv klsnkvvwfe tetvferkrc fadlmshvds
      121 ikkdqhrlke fqklwetakv fldkkyipep dvgleiygkm vinsycicna elssvgtgly
      181 lgpsildhsc vpnahavyeg hklllraaen iscdsvegir vsyidvmapk evrmqelssq
      241 yyflcsckrc cnkvpdcvse msstltasvk dkikelegnv verketlqql rqwseafqee
      301 lpvqdyaril aadllskccl gledykaalp pylerepiyr ecygqhspiy avllysiakl
      361 yhvtvqleka meyfkkaeav latshgrshp lymhlteayd hckfemenpg rgkgvtfkal
//
LOCUS       XP_077504499             358 aa            linear   INV 25-NOV-2025
DEFINITION  histone-lysine N-methyltransferase SMYD3-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504499
VERSION     XP_077504499.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648373.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..358
                     /product="histone-lysine N-methyltransferase SMYD3-like
                     isoform X2"
                     /calculated_mol_wt=40785
     Region          5..250
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(183..188,196..198,221..224)
                     /site_type="active"
                     /db_xref="CDD:380914"
     CDS             1..358
                     /gene="LOC144114554"
                     /coded_by="XM_077648373.1:31..1107"
                     /db_xref="GeneID:144114554"
ORIGIN      
        1 mltykagdei lsstpfvyvl gresrgrvcd ycfsecgnlk rcaackcvyy cnkpcqrqaw
       61 qdhkvecaal ckvapnvpet lvryfckllv klsnkvvwfe tetvferkrc fadlmshvds
      121 ikkdqhrlke fqklwetakv fldkkyipep dvgleiygkm vinsycicna elssvgtgly
      181 lgpsildhsc vpnahavyeg hklllraaen iscdsvegir vsyidvmapk evrmqelssq
      241 yyflcsckrc cnkvpdcvse msstltasvk dkikelegnv verketlqql rqwseafqee
      301 lpvqdyaril aadllskccl gledykaalp pylerepiyr lklywppatd vpihstct
//
LOCUS       XP_077504500             384 aa            linear   INV 25-NOV-2025
DEFINITION  histone-lysine N-methyltransferase SMYD3-like [Amblyomma
            americanum].
ACCESSION   XP_077504500
VERSION     XP_077504500.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648374.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..384
                     /product="histone-lysine N-methyltransferase SMYD3-like"
                     /calculated_mol_wt=43352
     Region          <10..38
                     /region_name="zf-MYND"
                     /note="MYND finger; pfam01753"
                     /db_xref="CDD:460312"
     Region          <53..222
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(154..159,167..169,192..195)
                     /site_type="active"
                     /db_xref="CDD:380914"
     CDS             1..384
                     /gene="LOC144114555"
                     /coded_by="XM_077648374.1:64..1218"
                     /db_xref="GeneID:144114555"
ORIGIN      
        1 mstvngcdsl kscsrcrvah ycnklcqasa lsehlpeccy fedvfpdipd vtvrlltriv
       61 hklqkermkd ckemvqghmr tledmeshan eimndpvtlp kfhaawatvk yilgennvpp
      121 lldgleifgr mvinrfcitd nelkpvgqgl yigasifdhs cdpdaayvfe gtrltvratr
      181 slsvnsvrei yicyvdklll kedriaaler qyyftcncaf cqkdareply ekwsgltecv
      241 rnlqleleka enegdveria srcreilvcq gkelpdndva kvealllafq cgarlatyae
      301 aigyalqame vmrtcygmys piyglhllhi grlyndsgnn vsaenflqma ssslavshgt
      361 thttyrelqr lrklnrraip ggck
//
LOCUS       XP_077504502             404 aa            linear   INV 25-NOV-2025
DEFINITION  dual specificity mitogen-activated protein kinase kinase 7-like
            [Amblyomma americanum].
ACCESSION   XP_077504502
VERSION     XP_077504502.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648376.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..404
                     /product="dual specificity mitogen-activated protein
                     kinase kinase 7-like"
                     /calculated_mol_wt=45510
     Region          104..399
                     /region_name="PKc_MKK7"
                     /note="Catalytic domain of the dual-specificity Protein
                     Kinase, Mitogen-activated protein Kinase Kinase 7;
                     cd06618"
                     /db_xref="CDD:270791"
     Site            order(124..128,132,145,147,178,194..197,199..200,203,242,
                     244..247,249,259..260,263,276..279,281,313,322)
                     /site_type="active"
                     /db_xref="CDD:270791"
     Site            order(124..128,130,132,145,147,178,194..197,199..200,
                     202..203,244,246..247,249,260)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270791"
     Site            order(127..128,242,244..246,263,276..279,281,313,322)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270791"
     Site            259..281
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270791"
     CDS             1..404
                     /gene="LOC144114556"
                     /coded_by="XM_077648376.1:55..1269"
                     /db_xref="GeneID:144114556"
ORIGIN      
        1 mieleipaqr paknkladlq arlraendak kkdklprdln lnfsspqhtp rsrprpfqpn
       61 emaapsrpak qfslptsspt itrqdsggds esklkeilka gnvltiggqq hkvemkdlqk
      121 lgdlgsgtcg hvvkmrhspt skvlavkqmr rsgnheenkr itmdlevvlr crdcphivqc
      181 lgylmtegev wicmelmatc ldkllrrlrp ralpedilgk talgvlralh ylkeshdlih
      241 rdvkpsnvll drqgrvrlcd fgisgrlvds kartrsagca aymaperidp pdptkpdydi
      301 radvwslgis mvelatgqfp yqdcktdfev lsrvlqdepp slpddgtfss efcsfvkqcl
      361 tkdynkrpky kkllehpfik ryerkevdva awlasvmdss qegq
//
LOCUS       XP_077504503             225 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114557 [Amblyomma americanum].
ACCESSION   XP_077504503
VERSION     XP_077504503.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648377.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..225
                     /product="uncharacterized protein LOC144114557"
                     /calculated_mol_wt=24589
     Region          69..181
                     /region_name="DUF4773"
                     /note="Domain of unknown function (DUF4773); pfam15998"
                     /db_xref="CDD:464965"
     CDS             1..225
                     /gene="LOC144114557"
                     /coded_by="XM_077648377.1:139..816"
                     /db_xref="GeneID:144114557"
ORIGIN      
        1 mspsavlvll allatsavlg gsarvheqvw gadplsglss qleeaeevrg evekeanktv
       61 vwpsdgrrce cignecgccl nisaprfgik qkacsnltyy pgqyalsfti siddsvvlnr
      121 tlsgrnpppv cwpeqlaqlc vrfynmtyhr tevdgcvrle gviarevivs ydvgcyvldd
      181 davktrisgl rkllvdairr aprpgfwpes vndlddatdr aptnr
//
LOCUS       XP_077504504             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114558 [Amblyomma americanum].
ACCESSION   XP_077504504
VERSION     XP_077504504.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648378.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144114558"
                     /calculated_mol_wt=14097
     CDS             1..127
                     /gene="LOC144114558"
                     /coded_by="XM_077648378.1:179..562"
                     /db_xref="GeneID:144114558"
ORIGIN      
        1 mrttellhgn grrpaeppip frqllplqlk dkvsgksgkq kdvaclpemm tlfsclaekn
       61 ydsnncskev kafqgcfdkf leknaeyrat gslgiltpgl kasqltsqqa ntllkkyplk
      121 nllkkir
//
LOCUS       XP_077504505             605 aa            linear   INV 25-NOV-2025
DEFINITION  tyrosine kinase receptor Cad96Ca-like [Amblyomma americanum].
ACCESSION   XP_077504505
VERSION     XP_077504505.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648379.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..605
                     /product="tyrosine kinase receptor Cad96Ca-like"
                     /calculated_mol_wt=67001
     Region          304..574
                     /region_name="PTKc"
                     /note="Catalytic domain of Protein Tyrosine Kinases;
                     cd00192"
                     /db_xref="CDD:270623"
     Site            order(306..310,314,332,334,381..384,387..388,440,444..445,
                     447,458,478..482,491,525)
                     /site_type="active"
                     /db_xref="CDD:270623"
     Site            order(306..307,309..310,314,332,334,381..384,387..388,
                     444..445,447,458)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270623"
     Site            order(440,444,478..482,491,525)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270623"
     Site            order(457..464,478..484)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270623"
     CDS             1..605
                     /gene="LOC144114559"
                     /coded_by="XM_077648379.1:406..2223"
                     /db_xref="GeneID:144114559"
ORIGIN      
        1 mvylgrtvds scelvaalav atlivvaats appplgnfqa lqasessega nsvpredada
       61 rvkverlqdn steieqetrn ssenrrvddk sksetptttp vdkdeplrrp dvdaedvtil
      121 dydsspaalg ptsseesrme alramesvti rpdddlaerk dqqqqgftke nsgtlvargp
      181 epdrsrrpey diespasggt nvyivaivgv vpaagaviwc vrkmiaknqe nkeraevpat
      241 gdedkqtpaq dpptkysyiq daynkmyakt iselmptssa pprasapvag spdelprdgv
      301 rlervlgegn fgrvwkgtay glngskratp vavktlkeya sneekrdlvr emglmrmlgk
      361 hphvvrflgc ctldepllli melvsrgklq sflrehrsrg ayynepeddg slgprdlaqf
      421 alqvaqgmdy ihkngiihrd laarnvlvde hgrckvadfg lsrsirdset dvyqqkskga
      481 lpvrwmapec lylqvfttks dvwafgilmw eivtlgstpy pglsaqevih avrdgrvmaq
      541 pvhcrwelyr lmracwhpdp kerppfsrla sdldqlikln agyidldnfp ehayynieag
      601 adekl
//
LOCUS       XP_077504506             317 aa            linear   INV 25-NOV-2025
DEFINITION  acyl-CoA Delta-9 desaturase-like [Amblyomma americanum].
ACCESSION   XP_077504506
VERSION     XP_077504506.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648380.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..317
                     /product="acyl-CoA Delta-9 desaturase-like"
                     /calculated_mol_wt=37030
     Region          1..298
                     /region_name="OLE1"
                     /note="Fatty-acid desaturase [Lipid transport and
                     metabolism]; COG1398"
                     /db_xref="CDD:441008"
     CDS             1..317
                     /gene="LOC144114561"
                     /coded_by="XM_077648380.1:376..1329"
                     /db_xref="GeneID:144114561"
ORIGIN      
        1 mstvtetvte erpapkpykm eivwrnvilm gslhlvsiyg fyliffsvqw ktvlaayily
       61 tlsgigitag shrlwshrsy kaklpyrimm milqtmafqn diyewardhr mhhkfsetta
      121 dphdatrgff fshvgwllvr khpdirnkgk aidlsdlmed pvvrfqkkyy lplmasicfi
      181 lpalgpwylw getlwnsfvv csltrycftl nmtwlvnsaa hmwgnrpydr hisprqnlvt
      241 ivgahgegfh nyhhtfpydy rtselgcrin tttwfidffa wlgqvydrke vptnvvqrrm
      301 ertgdgsrgl tagtrsy
//
LOCUS       XP_077504507             507 aa            linear   INV 25-NOV-2025
DEFINITION  ras-specific guanine nucleotide-releasing factor RalGPS1-like
            isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504507
VERSION     XP_077504507.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648381.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..507
                     /product="ras-specific guanine nucleotide-releasing factor
                     RalGPS1-like isoform X1"
                     /calculated_mol_wt=55986
     Region          58..221
                     /region_name="RasGEF"
                     /note="RasGEF domain; pfam00617"
                     /db_xref="CDD:459872"
     Region          397..503
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     CDS             1..507
                     /gene="LOC144114562"
                     /coded_by="XM_077648381.1:353..1876"
                     /db_xref="GeneID:144114562"
ORIGIN      
        1 mkygamvpad trgvqtdssh iteakrwpcd gsvwslrseq plnncegtld vvkveadela
       61 nqltlldlqa flkihpqela gcgwnrrekl qlapnvvalt nrfnhvslwv vqsvlcerlv
      121 grraellghf vrlaqqlqqi gnlhgacaiv salqsaplfr ltktwaqvar rerqllqrla
      181 alfseednfg sqrsrlrsal argspfvphl gpylrdllhm elcapqsdrg aanvslvgcl
      241 gtlqsscqrl tlipnaslqa qlvqpfylee lqkflqeday reslrleplg rqasltllap
      301 psepsptsgs tnrpprftpg hrkarslgtn vllsgpstts ngwgdaatpe qkgrllldds
      361 vaegagavlw rpaalelwep paplygweee lttqpascqg clrrktllke grrpamsswl
      421 rywvelrgns lvfyspksll akersdfkrq pckqsciagw lvlacpdads fqlldpsrgs
      481 rykfrpavgs sahdwcaclr asagttl
//
LOCUS       XP_077504508             507 aa            linear   INV 25-NOV-2025
DEFINITION  ras-specific guanine nucleotide-releasing factor RalGPS1-like
            isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504508
VERSION     XP_077504508.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648382.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..507
                     /product="ras-specific guanine nucleotide-releasing factor
                     RalGPS1-like isoform X2"
                     /calculated_mol_wt=55987
     Region          58..221
                     /region_name="RasGEF"
                     /note="RasGEF domain; pfam00617"
                     /db_xref="CDD:459872"
     Region          397..503
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     CDS             1..507
                     /gene="LOC144114562"
                     /coded_by="XM_077648382.1:359..1882"
                     /db_xref="GeneID:144114562"
ORIGIN      
        1 mkygamvpad trgvqtdssh iteaerwpcd gsvwslrseq plnncegtld vvkveadela
       61 nqltlldlqa flkihpqela gcgwnrrekl qlapnvvalt nrfnhvslwv vqsvlcerlv
      121 grraellghf vrlaqqlqqi gnlhgacaiv salqsaplfr ltktwaqvar rerqllqrla
      181 alfseednfg sqrsrlrsal argspfvphl gpylrdllhm elcapqsdrg aanvslvgcl
      241 gtlqsscqrl tlipnaslqa qlvqpfylee lqkflqeday reslrleplg rqasltllap
      301 psepsptsgs tnrpprftpg hrkarslgtn vllsgpstts ngwgdaatpe qkgrllldds
      361 vaegagavlw rpaalelwep paplygweee lttqpascqg clrrktllke grrpamsswl
      421 rywvelrgns lvfyspksll akersdfkrq pckqsciagw lvlacpdads fqlldpsrgs
      481 rykfrpavgs sahdwcaclr asagttl
//
LOCUS       XP_077504509             341 aa            linear   INV 25-NOV-2025
DEFINITION  stearoyl-CoA desaturase 5-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504509
VERSION     XP_077504509.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648383.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..341
                     /product="stearoyl-CoA desaturase 5-like isoform X1"
                     /calculated_mol_wt=39969
     Region          25..321
                     /region_name="OLE1"
                     /note="Fatty-acid desaturase [Lipid transport and
                     metabolism]; COG1398"
                     /db_xref="CDD:441008"
     CDS             1..341
                     /gene="LOC144114563"
                     /coded_by="XM_077648383.1:87..1112"
                     /db_xref="GeneID:144114563"
ORIGIN      
        1 mrdsgdseff khdhlwrwkg erramstvte tvteerpask phkmeivwrn vilmgslhli
       61 siygfyliff aaqwktvlaa yifytisgig vtagshrlws hrsykaklpy rimlmifqtm
      121 afqndiydwa rdhrmhhkfs ettadphdat rgfffshvgw llvrkhpdvr nkgksidlsd
      181 vladpvvrfq rryylplmvt icfivpallp wwlwgetlwn sfvvcsltry cftlnmtwlv
      241 nsaahiwgnr pydrhisprq nlvtivgahg egfhnyhhtf pydyrtselg crintttwfi
      301 dffawlgqvy drkevptsvv egrmkrtgdg srgltagtrs w
//
LOCUS       XP_077504510             317 aa            linear   INV 25-NOV-2025
DEFINITION  stearoyl-CoA desaturase 5-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504510
VERSION     XP_077504510.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648384.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..317
                     /product="stearoyl-CoA desaturase 5-like isoform X2"
                     /calculated_mol_wt=36940
     Region          1..297
                     /region_name="OLE1"
                     /note="Fatty-acid desaturase [Lipid transport and
                     metabolism]; COG1398"
                     /db_xref="CDD:441008"
     CDS             1..317
                     /gene="LOC144114563"
                     /coded_by="XM_077648384.1:464..1417"
                     /db_xref="GeneID:144114563"
ORIGIN      
        1 mstvtetvte erpaskphkm eivwrnvilm gslhlisiyg fyliffaaqw ktvlaayify
       61 tisgigvtag shrlwshrsy kaklpyriml mifqtmafqn diydwardhr mhhkfsetta
      121 dphdatrgff fshvgwllvr khpdvrnkgk sidlsdvlad pvvrfqrryy lplmvticfi
      181 vpallpwwlw getlwnsfvv csltrycftl nmtwlvnsaa hiwgnrpydr hisprqnlvt
      241 ivgahgegfh nyhhtfpydy rtselgcrin tttwfidffa wlgqvydrke vptsvvegrm
      301 krtgdgsrgl tagtrsw
//
LOCUS       XP_077504511            2194 aa            linear   INV 25-NOV-2025
DEFINITION  calcium voltage-gated channel subunit cacophony isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077504511
VERSION     XP_077504511.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648385.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2194
                     /product="calcium voltage-gated channel subunit cacophony
                     isoform X1"
                     /calculated_mol_wt=245736
     Region          <56..>154
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     Region          288..577
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          707..938
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1069..1302
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1392..1641
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1650..1703
                     /region_name="GPHH"
                     /note="Voltage-dependent L-type calcium channel,
                     IQ-associated; pfam16905"
                     /db_xref="CDD:465306"
     Region          1713..1792
                     /region_name="Ca_chan_IQ"
                     /note="Voltage gated calcium channel IQ domain; pfam08763"
                     /db_xref="CDD:462591"
     Region          1967..>2171
                     /region_name="PHA03381"
                     /note="tegument protein VP22; Provisional"
                     /db_xref="CDD:177618"
     CDS             1..2194
                     /gene="cac"
                     /coded_by="XM_077648385.1:64..6648"
                     /db_xref="GeneID:144114565"
ORIGIN      
        1 msgflrfprl vreriserrk rrlrarqrar svavegpgca mtgpttgrpg ggrrgsaplp
       61 vqrraslspq ppgvsaptsl gpspkqsprp svegspkesp psaeqdgpps pggaspaqst
      121 tsalatpqdp aaaaehkqrp tcappggsss lavtaaaapa psprtsrrrr apsrlstelp
      181 agvtlgrrra vtttdhktca llhtrlkgir ledialsals aesggergll rapetsgvlg
      241 ghgsgalrte pgmarrkgld prargpsslf llsednllrr ytkffiewpp leyavlltii
      301 ancivlalee hlpksdrtpl aqklektepy flgifcveal lkivalgfil hrgaylrniw
      361 nimdfvvvvt gfvtyfvped ldldlrtlra irvlrplklv sgipslqvvl ksiikamapl
      421 lqigllvlfa ivifaiigle fysgalhktc ysledaneiv pegeqetpcy qdspinsshp
      481 sgayicdhnt sickegwigp nygitsfdni ffamltvfqc itmegwtail ywtndalgns
      541 fnwvyfvpli ilgsffmlnl vlgvlsgefa kerervenrq aflkirrqqq lereldgyve
      601 wickaeevil aeertteeek mhimearrra ankrkklksm hnkstdeeee eevedeetpa
      661 gkvklkgfar asylktkikn kgacrafwka ekqfrffirq vvktqtfywv vivlvflntv
      721 cvavehhdqd qyltdflyya efaflglfif emlikvyalg priyfessfn rfdcvviags
      781 ifevvwsslk egsfglsvlr alrllrifkf tkywsslrnl visllnsmrs iisllfllfl
      841 filifallgm qlfggvfnfp egtpaanfnt fpialltvfq iltgedwnev myngiqsqdg
      901 ihggmiysly fiilvlfgny tllnvflaia vdnlanaqel taaeeedaee ekerakeele
      961 keiadlqmat tgsldppqvn icppspqsga pgkgktfpfq enqleagskf kgtndnnnid
     1021 vdedyappre dddedeitgp kpmmpyscmf icsptnpirr aahfivnlry felfimitit
     1081 lssialaaed pveedstknr ilnyfdyaft gvftvemllk iadqgvifhp gaycrdswni
     1141 ldaivvical iafafagsst gqnlstiksl rvlrvlrplk tikrvpklka vfdcvvtslk
     1201 nvfnilivyi lfqfifavis vqlfngkfhy ctdaskltqm eckgeyyvfs dadkppkver
     1261 rqwkhqpfhy dnvmaamltl ftvqtgegwp qvlqnsmdst degkgplpry riemsifyvv
     1321 ffivfpfffv nifvaliiit fqeqgekele egeldknqks cidfaiqakp lqrympknkd
     1381 sfkykvwkiv vstpfeyfim vlivlntvll mmkfynqskt lkntlhymna aftalftiec
     1441 vlkvaafgvr nffknpwhtf dfitvigsii dvlvmetgln ffsvgflrlf raarlikllr
     1501 qgytirillw tfiqsfkalp yvclliamlf fiyaiigmqv fgnimlasds qitrhnnfrt
     1561 ffqglmllfr catgeawqai mmacvkgrpc dplskkdkpe cgsnmayayf vsfiffcsfl
     1621 mlnlfvavim dnfdyltrds silgahhlde firvwaeydp natgyihyte mydmlrnmdp
     1681 plgfgnkcpy rlaykklirm nmpvteegkv nfsttlfali renlsikmrp aeemdqadke
     1741 lkdtirklwp lharkvtdkf mppdselgag rltvgkiygg llilenwrtt rfgqapgags
     1801 ltekasppsp pdsprpsllq rlmgavrnsn trashgslnq deahlddghl qppsrhgslh
     1861 rsslsmerng qssekpwqrs lsflrrgssr rhrppqagpp plpptappap ekvgagpgag
     1921 lpaavarcmr rtctppggas aapppptppg qhpphcarap repprepprl tlrrraqgdr
     1981 pparlrpdsi algpspqgsr ctsplrrsps prrsaagfsd avsdlvdkvk yetsrrgrar
     2041 arlhgdeflr dsplrgrrgs aeyrsstwlg qrsrspspsr aaerhyggar saspaasaqs
     2101 lpaqrrrlpp tpakpsrlrl darsmeagin fpsvsqsptl pgarspaain fpkvnaspth
     2161 cprqlpspll pngykprrtp rrrrsgdtdd ddwc
//
LOCUS       XP_077504512            2194 aa            linear   INV 25-NOV-2025
DEFINITION  calcium voltage-gated channel subunit cacophony isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077504512
VERSION     XP_077504512.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648386.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2194
                     /product="calcium voltage-gated channel subunit cacophony
                     isoform X2"
                     /calculated_mol_wt=245918
     Region          <56..>154
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     Region          288..577
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          707..938
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1069..1302
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1392..1641
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1650..1703
                     /region_name="GPHH"
                     /note="Voltage-dependent L-type calcium channel,
                     IQ-associated; pfam16905"
                     /db_xref="CDD:465306"
     Region          1713..1792
                     /region_name="Ca_chan_IQ"
                     /note="Voltage gated calcium channel IQ domain; pfam08763"
                     /db_xref="CDD:462591"
     Region          1967..>2171
                     /region_name="PHA03381"
                     /note="tegument protein VP22; Provisional"
                     /db_xref="CDD:177618"
     CDS             1..2194
                     /gene="cac"
                     /coded_by="XM_077648386.1:64..6648"
                     /db_xref="GeneID:144114565"
ORIGIN      
        1 msgflrfprl vreriserrk rrlrarqrar svavegpgca mtgpttgrpg ggrrgsaplp
       61 vqrraslspq ppgvsaptsl gpspkqsprp svegspkesp psaeqdgpps pggaspaqst
      121 tsalatpqdp aaaaehkqrp tcappggsss lavtaaaapa psprtsrrrr apsrlstelp
      181 agvtlgrrra vtttdhktca llhtrlkgir ledialsals aesggergll rapetsgvlg
      241 ghgsgalrte pgmarrkgld prargpsslf llsednllrr ytkffiewpp leyavlltii
      301 ancivlalee hlpksdrtpl aqklektepy flgifcveal lkivalgfil hrgaylrniw
      361 nimdfvvvvt gfvtyfvped ldldlrtlra irvlrplklv sgipslqvvl ksiikamapl
      421 lqigllvlfa ivifaiigle fysgalhktc ysledaneiv pegeqetpcy qdspinsshp
      481 sgayicdhnt sickegwigp nygitsfdni ffamltvfqc itmegwtail ywtndalgns
      541 fnwvyfvpli ilgsffmlnl vlgvlsgefa kerervenrq aflkirrqqq lereldgyve
      601 wickaeevil aeertteeek mhimearrra ankrkklksm hnkstdeeee eevedeetpa
      661 gkvklkgfar asylktkikn kgacrafwka ekqfrffirq vvktqtfywv vivlvflntv
      721 cvavehhdqd qyltdflyya efaflglfif emlikvyalg priyfessfn rfdcvviags
      781 ifevvwsslk egsfglsvlr alrllrifkf tkywsslrnl visllnsmrs iisllfllfl
      841 filifallgm qlfggvfnfp egtpaanfnt fpialltvfq iltgedwnev myngiqsqdg
      901 ihggmiysly fiilvlfgny tllnvflaia vdnlanaqel taaeeedaee ekerakeele
      961 keiadlqmat tgsldppqvn icppspqsga pgkgktfpfq enqleagskf kgtndnnnid
     1021 vdedyappre dddedeitgp kpmmpyscmf icsptnpirr aahfivnlry felfimitit
     1081 lssialaaed pveedstknr ilnyfdyaft gvftvemllk iadqgvifhp gaycrdswni
     1141 ldaivvical iafafagsst gqnlstiksl rvlrvlrplk tikrvpklka vfdcvvtslk
     1201 nvfnilivyi lfqfifavis vqlfngkfhy ctdaskltqm eckgeyyvfs dadkppkver
     1261 rqwkhqpfhy dnvmaamltl ftvqtgegwp qvlqnsmdst degkgplpry riemsifyvv
     1321 ffivfpfffv nifvaliiit fqeqgekele egeldknqks cidfaiqakp lqrympknkd
     1381 sfkykvwkiv vstpfeyfim vlivlntvll mmkfhyasdl ysdilsylnm aftilfllec
     1441 ifkfmaygck nffknpwhtf dfitvigsii dvlvmetgln ffsvgflrlf raarlikllr
     1501 qgytirillw tfiqsfkalp yvclliamlf fiyaiigmqv fgnimlasds qitrhnnfrt
     1561 ffqglmllfr catgeawqai mmacvkgrpc dplskkdkpe cgsnmayayf vsfiffcsfl
     1621 mlnlfvavim dnfdyltrds silgahhlde firvwaeydp natgyihyte mydmlrnmdp
     1681 plgfgnkcpy rlaykklirm nmpvteegkv nfsttlfali renlsikmrp aeemdqadke
     1741 lkdtirklwp lharkvtdkf mppdselgag rltvgkiygg llilenwrtt rfgqapgags
     1801 ltekasppsp pdsprpsllq rlmgavrnsn trashgslnq deahlddghl qppsrhgslh
     1861 rsslsmerng qssekpwqrs lsflrrgssr rhrppqagpp plpptappap ekvgagpgag
     1921 lpaavarcmr rtctppggas aapppptppg qhpphcarap repprepprl tlrrraqgdr
     1981 pparlrpdsi algpspqgsr ctsplrrsps prrsaagfsd avsdlvdkvk yetsrrgrar
     2041 arlhgdeflr dsplrgrrgs aeyrsstwlg qrsrspspsr aaerhyggar saspaasaqs
     2101 lpaqrrrlpp tpakpsrlrl darsmeagin fpsvsqsptl pgarspaain fpkvnaspth
     2161 cprqlpspll pngykprrtp rrrrsgdtdd ddwc
//
LOCUS       XP_077504513            2184 aa            linear   INV 25-NOV-2025
DEFINITION  calcium voltage-gated channel subunit cacophony isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077504513
VERSION     XP_077504513.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648387.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2184
                     /product="calcium voltage-gated channel subunit cacophony
                     isoform X3"
                     /calculated_mol_wt=244684
     Region          <56..>154
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     Region          288..577
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          697..928
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1059..1292
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1382..1631
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1640..1693
                     /region_name="GPHH"
                     /note="Voltage-dependent L-type calcium channel,
                     IQ-associated; pfam16905"
                     /db_xref="CDD:465306"
     Region          1703..1782
                     /region_name="Ca_chan_IQ"
                     /note="Voltage gated calcium channel IQ domain; pfam08763"
                     /db_xref="CDD:462591"
     Region          1957..>2161
                     /region_name="PHA03381"
                     /note="tegument protein VP22; Provisional"
                     /db_xref="CDD:177618"
     CDS             1..2184
                     /gene="cac"
                     /coded_by="XM_077648387.1:64..6618"
                     /db_xref="GeneID:144114565"
ORIGIN      
        1 msgflrfprl vreriserrk rrlrarqrar svavegpgca mtgpttgrpg ggrrgsaplp
       61 vqrraslspq ppgvsaptsl gpspkqsprp svegspkesp psaeqdgpps pggaspaqst
      121 tsalatpqdp aaaaehkqrp tcappggsss lavtaaaapa psprtsrrrr apsrlstelp
      181 agvtlgrrra vtttdhktca llhtrlkgir ledialsals aesggergll rapetsgvlg
      241 ghgsgalrte pgmarrkgld prargpsslf llsednllrr ytkffiewpp leyavlltii
      301 ancivlalee hlpksdrtpl aqklektepy flgifcveal lkivalgfil hrgaylrniw
      361 nimdfvvvvt gfvtyfvped ldldlrtlra irvlrplklv sgipslqvvl ksiikamapl
      421 lqigllvlfa ivifaiigle fysgalhktc ysledaneiv pegeqetpcy qdspinsshp
      481 sgayicdhnt sickegwigp nygitsfdni ffamltvfqc itmegwtail ywtndalgns
      541 fnwvyfvpli ilgsffmlnl vlgvlsgefa kerervenrq aflkirrqqq lereldgyve
      601 wickaeevil aeertteeek mhimearrra ankrkklksm hnkstdeeee eevedegfar
      661 asylktkikn kgacrafwka ekqfrffirq vvktqtfywv vivlvflntv cvavehhdqd
      721 qyltdflyya efaflglfif emlikvyalg priyfessfn rfdcvviags ifevvwsslk
      781 egsfglsvlr alrllrifkf tkywsslrnl visllnsmrs iisllfllfl filifallgm
      841 qlfggvfnfp egtpaanfnt fpialltvfq iltgedwnev myngiqsqdg ihggmiysly
      901 fiilvlfgny tllnvflaia vdnlanaqel taaeeedaee ekerakeele keiadlqmat
      961 tgsldppqvn icppspqsga pgkgktfpfq enqleagskf kgtndnnnid vdedyappre
     1021 dddedeitgp kpmmpyscmf icsptnpirr aahfivnlry felfimitit lssialaaed
     1081 pveedstknr ilnyfdyaft gvftvemllk iadqgvifhp gaycrdswni ldaivvical
     1141 iafafagsst gqnlstiksl rvlrvlrplk tikrvpklka vfdcvvtslk nvfnilivyi
     1201 lfqfifavis vqlfngkfhy ctdaskltqm eckgeyyvfs dadkppkver rqwkhqpfhy
     1261 dnvmaamltl ftvqtgegwp qvlqnsmdst degkgplpry riemsifyvv ffivfpfffv
     1321 nifvaliiit fqeqgekele egeldknqks cidfaiqakp lqrympknkd sfkykvwkiv
     1381 vstpfeyfim vlivlntvll mmkfynqskt lkntlhymna aftalftiec vlkvaafgvr
     1441 nffknpwhtf dfitvigsii dvlvmetgln ffsvgflrlf raarlikllr qgytirillw
     1501 tfiqsfkalp yvclliamlf fiyaiigmqv fgnimlasds qitrhnnfrt ffqglmllfr
     1561 catgeawqai mmacvkgrpc dplskkdkpe cgsnmayayf vsfiffcsfl mlnlfvavim
     1621 dnfdyltrds silgahhlde firvwaeydp natgyihyte mydmlrnmdp plgfgnkcpy
     1681 rlaykklirm nmpvteegkv nfsttlfali renlsikmrp aeemdqadke lkdtirklwp
     1741 lharkvtdkf mppdselgag rltvgkiygg llilenwrtt rfgqapgags ltekasppsp
     1801 pdsprpsllq rlmgavrnsn trashgslnq deahlddghl qppsrhgslh rsslsmerng
     1861 qssekpwqrs lsflrrgssr rhrppqagpp plpptappap ekvgagpgag lpaavarcmr
     1921 rtctppggas aapppptppg qhpphcarap repprepprl tlrrraqgdr pparlrpdsi
     1981 algpspqgsr ctsplrrsps prrsaagfsd avsdlvdkvk yetsrrgrar arlhgdeflr
     2041 dsplrgrrgs aeyrsstwlg qrsrspspsr aaerhyggar saspaasaqs lpaqrrrlpp
     2101 tpakpsrlrl darsmeagin fpsvsqsptl pgarspaain fpkvnaspth cprqlpspll
     2161 pngykprrtp rrrrsgdtdd ddwc
//
LOCUS       XP_077504514            2182 aa            linear   INV 25-NOV-2025
DEFINITION  calcium voltage-gated channel subunit cacophony isoform X4
            [Amblyomma americanum].
ACCESSION   XP_077504514
VERSION     XP_077504514.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648388.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2182
                     /product="calcium voltage-gated channel subunit cacophony
                     isoform X4"
                     /calculated_mol_wt=244546
     Region          <56..>154
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     Region          288..577
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          707..938
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1069..1302
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1392..1641
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1650..1703
                     /region_name="GPHH"
                     /note="Voltage-dependent L-type calcium channel,
                     IQ-associated; pfam16905"
                     /db_xref="CDD:465306"
     Region          1713..1792
                     /region_name="Ca_chan_IQ"
                     /note="Voltage gated calcium channel IQ domain; pfam08763"
                     /db_xref="CDD:462591"
     Region          1955..>2159
                     /region_name="PHA03381"
                     /note="tegument protein VP22; Provisional"
                     /db_xref="CDD:177618"
     CDS             1..2182
                     /gene="cac"
                     /coded_by="XM_077648388.1:64..6612"
                     /db_xref="GeneID:144114565"
ORIGIN      
        1 msgflrfprl vreriserrk rrlrarqrar svavegpgca mtgpttgrpg ggrrgsaplp
       61 vqrraslspq ppgvsaptsl gpspkqsprp svegspkesp psaeqdgpps pggaspaqst
      121 tsalatpqdp aaaaehkqrp tcappggsss lavtaaaapa psprtsrrrr apsrlstelp
      181 agvtlgrrra vtttdhktca llhtrlkgir ledialsals aesggergll rapetsgvlg
      241 ghgsgalrte pgmarrkgld prargpsslf llsednllrr ytkffiewpp leyavlltii
      301 ancivlalee hlpksdrtpl aqklektepy flgifcveal lkivalgfil hrgaylrniw
      361 nimdfvvvvt gfvtyfvped ldldlrtlra irvlrplklv sgipslqvvl ksiikamapl
      421 lqigllvlfa ivifaiigle fysgalhktc ysledaneiv pegeqetpcy qdspinsshp
      481 sgayicdhnt sickegwigp nygitsfdni ffamltvfqc itmegwtail ywtndalgns
      541 fnwvyfvpli ilgsffmlnl vlgvlsgefa kerervenrq aflkirrqqq lereldgyve
      601 wickaeevil aeertteeek mhimearrra ankrkklksm hnkstdeeee eevedeetpa
      661 gkvklkgfar asylktkikn kgacrafwka ekqfrffirq vvktqtfywv vivlvflntv
      721 cvavehhdqd qyltdflyya efaflglfif emlikvyalg priyfessfn rfdcvviags
      781 ifevvwsslk egsfglsvlr alrllrifkf tkywsslrnl visllnsmrs iisllfllfl
      841 filifallgm qlfggvfnfp egtpaanfnt fpialltvfq iltgedwnev myngiqsqdg
      901 ihggmiysly fiilvlfgny tllnvflaia vdnlanaqel taaeeedaee ekerakeele
      961 keiadlqmat tgsldppqvn icppspqsga pgkgktfpfq enqleagskf kgtndnnnid
     1021 vdedyappre dddedeitgp kpmmpyscmf icsptnpirr aahfivnlry felfimitit
     1081 lssialaaed pveedstknr ilnyfdyaft gvftvemllk iadqgvifhp gaycrdswni
     1141 ldaivvical iafafagsst gqnlstiksl rvlrvlrplk tikrvpklka vfdcvvtslk
     1201 nvfnilivyi lfqfifavis vqlfngkfhy ctdaskltqm eckgeyyvfs dadkppkver
     1261 rqwkhqpfhy dnvmaamltl ftvqtgegwp qvlqnsmdst degkgplpry riemsifyvv
     1321 ffivfpfffv nifvaliiit fqeqgekele egeldknqks cidfaiqakp lqrympknkd
     1381 sfkykvwkiv vstpfeyfim vlivlntvll mmkfynqskt lkntlhymna aftalftiec
     1441 vlkvaafgvr nffknpwhtf dfitvigsii dvlvmetgln ffsvgflrlf raarlikllr
     1501 qgytirillw tfiqsfkalp yvclliamlf fiyaiigmqv fgnimlasds qitrhnnfrt
     1561 ffqglmllfr catgeawqai mmacvkgrpc dplskkdkpe cgsnmayayf vsfiffcsfl
     1621 mlnlfvavim dnfdyltrds silgahhlde firvwaeydp natgyihyte mydmlrnmdp
     1681 plgfgnkcpy rlaykklirm nmpvteegkv nfsttlfali renlsikmrp aeemdqadke
     1741 lkdtirklwp lharkvtdkf mppdselgag rltvgkiygg llilenwrtt rfgqapgags
     1801 ltrpsllqrl mgavrnsntr ashgslnqde ahlddghlqp psrhgslhrs slsmerngqs
     1861 sekpwqrsls flrrgssrrh rppqagpppl pptappapek vgagpgaglp aavarcmrrt
     1921 ctppggasaa pppptppgqh pphcarapre pprepprltl rrraqgdrpp arlrpdsial
     1981 gpspqgsrct splrrspspr rsaagfsdav sdlvdkvkye tsrrgrarar lhgdeflrds
     2041 plrgrrgsae yrsstwlgqr srspspsraa erhyggarsa spaasaqslp aqrrrlpptp
     2101 akpsrlrlda rsmeaginfp svsqsptlpg arspaainfp kvnaspthcp rqlpspllpn
     2161 gykprrtprr rrsgdtdddd wc
//
LOCUS       XP_077504515            2157 aa            linear   INV 25-NOV-2025
DEFINITION  calcium voltage-gated channel subunit cacophony isoform X5
            [Amblyomma americanum].
ACCESSION   XP_077504515
VERSION     XP_077504515.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648389.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2157
                     /product="calcium voltage-gated channel subunit cacophony
                     isoform X5"
                     /calculated_mol_wt=241542
     Region          <56..>154
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     Region          288..577
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          707..938
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1069..1302
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1392..1604
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1613..1666
                     /region_name="GPHH"
                     /note="Voltage-dependent L-type calcium channel,
                     IQ-associated; pfam16905"
                     /db_xref="CDD:465306"
     Region          1676..1755
                     /region_name="Ca_chan_IQ"
                     /note="Voltage gated calcium channel IQ domain; pfam08763"
                     /db_xref="CDD:462591"
     Region          1930..>2134
                     /region_name="PHA03381"
                     /note="tegument protein VP22; Provisional"
                     /db_xref="CDD:177618"
     CDS             1..2157
                     /gene="cac"
                     /coded_by="XM_077648389.1:64..6537"
                     /db_xref="GeneID:144114565"
ORIGIN      
        1 msgflrfprl vreriserrk rrlrarqrar svavegpgca mtgpttgrpg ggrrgsaplp
       61 vqrraslspq ppgvsaptsl gpspkqsprp svegspkesp psaeqdgpps pggaspaqst
      121 tsalatpqdp aaaaehkqrp tcappggsss lavtaaaapa psprtsrrrr apsrlstelp
      181 agvtlgrrra vtttdhktca llhtrlkgir ledialsals aesggergll rapetsgvlg
      241 ghgsgalrte pgmarrkgld prargpsslf llsednllrr ytkffiewpp leyavlltii
      301 ancivlalee hlpksdrtpl aqklektepy flgifcveal lkivalgfil hrgaylrniw
      361 nimdfvvvvt gfvtyfvped ldldlrtlra irvlrplklv sgipslqvvl ksiikamapl
      421 lqigllvlfa ivifaiigle fysgalhktc ysledaneiv pegeqetpcy qdspinsshp
      481 sgayicdhnt sickegwigp nygitsfdni ffamltvfqc itmegwtail ywtndalgns
      541 fnwvyfvpli ilgsffmlnl vlgvlsgefa kerervenrq aflkirrqqq lereldgyve
      601 wickaeevil aeertteeek mhimearrra ankrkklksm hnkstdeeee eevedeetpa
      661 gkvklkgfar asylktkikn kgacrafwka ekqfrffirq vvktqtfywv vivlvflntv
      721 cvavehhdqd qyltdflyya efaflglfif emlikvyalg priyfessfn rfdcvviags
      781 ifevvwsslk egsfglsvlr alrllrifkf tkywsslrnl visllnsmrs iisllfllfl
      841 filifallgm qlfggvfnfp egtpaanfnt fpialltvfq iltgedwnev myngiqsqdg
      901 ihggmiysly fiilvlfgny tllnvflaia vdnlanaqel taaeeedaee ekerakeele
      961 keiadlqmat tgsldppqvn icppspqsga pgkgktfpfq enqleagskf kgtndnnnid
     1021 vdedyappre dddedeitgp kpmmpyscmf icsptnpirr aahfivnlry felfimitit
     1081 lssialaaed pveedstknr ilnyfdyaft gvftvemllk iadqgvifhp gaycrdswni
     1141 ldaivvical iafafagsst gqnlstiksl rvlrvlrplk tikrvpklka vfdcvvtslk
     1201 nvfnilivyi lfqfifavis vqlfngkfhy ctdaskltqm eckgeyyvfs dadkppkver
     1261 rqwkhqpfhy dnvmaamltl ftvqtgegwp qvlqnsmdst degkgplpry riemsifyvv
     1321 ffivfpfffv nifvaliiit fqeqgekele egeldknqks cidfaiqakp lqrympknkd
     1381 sfkykvwkiv vstpfeyfim vlivlntvll mmknffknpw htfdfitvig siidvlvmet
     1441 glnffsvgfl rlfraarlik llrqgytiri llwtfiqsfk alpyvcllia mlffiyaiig
     1501 mqvfgnimla sdsqitrhnn frtffqglml lfrcatgeaw qaimmacvkg rpcdplskkd
     1561 kpecgsnmay ayfvsfiffc sflmlnlfva vimdnfdylt rdssilgahh ldefirvwae
     1621 ydpnatgyih ytemydmlrn mdpplgfgnk cpyrlaykkl irmnmpvtee gkvnfsttlf
     1681 alirenlsik mrpaeemdqa dkelkdtirk lwplharkvt dkfmppdsel gagrltvgki
     1741 yggllilenw rttrfgqapg agsltekasp psppdsprps llqrlmgavr nsntrashgs
     1801 lnqdeahldd ghlqppsrhg slhrsslsme rngqssekpw qrslsflrrg ssrrhrppqa
     1861 gppplpptap papekvgagp gaglpaavar cmrrtctppg gasaappppt ppgqhpphca
     1921 raprepprep prltlrrraq gdrpparlrp dsialgpspq gsrctsplrr spsprrsaag
     1981 fsdavsdlvd kvkyetsrrg rararlhgde flrdsplrgr rgsaeyrsst wlgqrsrsps
     2041 psraaerhyg garsaspaas aqslpaqrrr lpptpakpsr lrldarsmea ginfpsvsqs
     2101 ptlpgarspa ainfpkvnas pthcprqlps pllpngykpr rtprrrrsgd tddddwc
//
LOCUS       XP_077504516            2154 aa            linear   INV 25-NOV-2025
DEFINITION  calcium voltage-gated channel subunit cacophony isoform X6
            [Amblyomma americanum].
ACCESSION   XP_077504516
VERSION     XP_077504516.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648390.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2154
                     /product="calcium voltage-gated channel subunit cacophony
                     isoform X6"
                     /calculated_mol_wt=241029
     Region          <17..104
                     /region_name="PHA03378"
                     /note="EBNA-3B; Provisional"
                     /db_xref="CDD:223065"
     Region          248..537
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          667..898
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1029..1262
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1352..1601
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1610..1663
                     /region_name="GPHH"
                     /note="Voltage-dependent L-type calcium channel,
                     IQ-associated; pfam16905"
                     /db_xref="CDD:465306"
     Region          1673..1752
                     /region_name="Ca_chan_IQ"
                     /note="Voltage gated calcium channel IQ domain; pfam08763"
                     /db_xref="CDD:462591"
     Region          1927..>2131
                     /region_name="PHA03381"
                     /note="tegument protein VP22; Provisional"
                     /db_xref="CDD:177618"
     CDS             1..2154
                     /gene="cac"
                     /coded_by="XM_077648390.1:205..6669"
                     /db_xref="GeneID:144114565"
ORIGIN      
        1 mtgpttgrpg ggrrgsaplp vqrraslspq ppgvsaptsl gpspkqsprp svegspkesp
       61 psaeqdgpps pggaspaqst tsalatpqdp aaaaehkqrp tcappggsss lavtaaaapa
      121 psprtsrrrr apsrlstelp agvtlgrrra vtttdhktca llhtrlkgir ledialsals
      181 aesggergll rapetsgvlg ghgsgalrte pgmarrkgld prargpsslf llsednllrr
      241 ytkffiewpp leyavlltii ancivlalee hlpksdrtpl aqklektepy flgifcveal
      301 lkivalgfil hrgaylrniw nimdfvvvvt gfvtyfvped ldldlrtlra irvlrplklv
      361 sgipslqvvl ksiikamapl lqigllvlfa ivifaiigle fysgalhktc ysledaneiv
      421 pegeqetpcy qdspinsshp sgayicdhnt sickegwigp nygitsfdni ffamltvfqc
      481 itmegwtail ywtndalgns fnwvyfvpli ilgsffmlnl vlgvlsgefa kerervenrq
      541 aflkirrqqq lereldgyve wickaeevil aeertteeek mhimearrra ankrkklksm
      601 hnkstdeeee eevedeetpa gkvklkgfar asylktkikn kgacrafwka ekqfrffirq
      661 vvktqtfywv vivlvflntv cvavehhdqd qyltdflyya efaflglfif emlikvyalg
      721 priyfessfn rfdcvviags ifevvwsslk egsfglsvlr alrllrifkf tkywsslrnl
      781 visllnsmrs iisllfllfl filifallgm qlfggvfnfp egtpaanfnt fpialltvfq
      841 iltgedwnev myngiqsqdg ihggmiysly fiilvlfgny tllnvflaia vdnlanaqel
      901 taaeeedaee ekerakeele keiadlqmat tgsldppqvn icppspqsga pgkgktfpfq
      961 enqleagskf kgtndnnnid vdedyappre dddedeitgp kpmmpyscmf icsptnpirr
     1021 aahfivnlry felfimitit lssialaaed pveedstknr ilnyfdyaft gvftvemllk
     1081 iadqgvifhp gaycrdswni ldaivvical iafafagsst gqnlstiksl rvlrvlrplk
     1141 tikrvpklka vfdcvvtslk nvfnilivyi lfqfifavis vqlfngkfhy ctdaskltqm
     1201 eckgeyyvfs dadkppkver rqwkhqpfhy dnvmaamltl ftvqtgegwp qvlqnsmdst
     1261 degkgplpry riemsifyvv ffivfpfffv nifvaliiit fqeqgekele egeldknqks
     1321 cidfaiqakp lqrympknkd sfkykvwkiv vstpfeyfim vlivlntvll mmkfynqskt
     1381 lkntlhymna aftalftiec vlkvaafgvr nffknpwhtf dfitvigsii dvlvmetgln
     1441 ffsvgflrlf raarlikllr qgytirillw tfiqsfkalp yvclliamlf fiyaiigmqv
     1501 fgnimlasds qitrhnnfrt ffqglmllfr catgeawqai mmacvkgrpc dplskkdkpe
     1561 cgsnmayayf vsfiffcsfl mlnlfvavim dnfdyltrds silgahhlde firvwaeydp
     1621 natgyihyte mydmlrnmdp plgfgnkcpy rlaykklirm nmpvteegkv nfsttlfali
     1681 renlsikmrp aeemdqadke lkdtirklwp lharkvtdkf mppdselgag rltvgkiygg
     1741 llilenwrtt rfgqapgags ltekasppsp pdsprpsllq rlmgavrnsn trashgslnq
     1801 deahlddghl qppsrhgslh rsslsmerng qssekpwqrs lsflrrgssr rhrppqagpp
     1861 plpptappap ekvgagpgag lpaavarcmr rtctppggas aapppptppg qhpphcarap
     1921 repprepprl tlrrraqgdr pparlrpdsi algpspqgsr ctsplrrsps prrsaagfsd
     1981 avsdlvdkvk yetsrrgrar arlhgdeflr dsplrgrrgs aeyrsstwlg qrsrspspsr
     2041 aaerhyggar saspaasaqs lpaqrrrlpp tpakpsrlrl darsmeagin fpsvsqsptl
     2101 pgarspaain fpkvnaspth cprqlpspll pngykprrtp rrrrsgdtdd ddwc
//
LOCUS       XP_077504517            2129 aa            linear   INV 25-NOV-2025
DEFINITION  calcium voltage-gated channel subunit cacophony isoform X7
            [Amblyomma americanum].
ACCESSION   XP_077504517
VERSION     XP_077504517.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648391.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2129
                     /product="calcium voltage-gated channel subunit cacophony
                     isoform X7"
                     /calculated_mol_wt=239084
     Region          <56..>154
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     Region          288..577
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          707..938
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1069..1302
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1392..1641
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1650..1703
                     /region_name="GPHH"
                     /note="Voltage-dependent L-type calcium channel,
                     IQ-associated; pfam16905"
                     /db_xref="CDD:465306"
     Region          1713..1792
                     /region_name="Ca_chan_IQ"
                     /note="Voltage gated calcium channel IQ domain; pfam08763"
                     /db_xref="CDD:462591"
     Region          1914..>2106
                     /region_name="PHA03381"
                     /note="tegument protein VP22; Provisional"
                     /db_xref="CDD:177618"
     CDS             1..2129
                     /gene="cac"
                     /coded_by="XM_077648391.1:64..6453"
                     /db_xref="GeneID:144114565"
ORIGIN      
        1 msgflrfprl vreriserrk rrlrarqrar svavegpgca mtgpttgrpg ggrrgsaplp
       61 vqrraslspq ppgvsaptsl gpspkqsprp svegspkesp psaeqdgpps pggaspaqst
      121 tsalatpqdp aaaaehkqrp tcappggsss lavtaaaapa psprtsrrrr apsrlstelp
      181 agvtlgrrra vtttdhktca llhtrlkgir ledialsals aesggergll rapetsgvlg
      241 ghgsgalrte pgmarrkgld prargpsslf llsednllrr ytkffiewpp leyavlltii
      301 ancivlalee hlpksdrtpl aqklektepy flgifcveal lkivalgfil hrgaylrniw
      361 nimdfvvvvt gfvtyfvped ldldlrtlra irvlrplklv sgipslqvvl ksiikamapl
      421 lqigllvlfa ivifaiigle fysgalhktc ysledaneiv pegeqetpcy qdspinsshp
      481 sgayicdhnt sickegwigp nygitsfdni ffamltvfqc itmegwtail ywtndalgns
      541 fnwvyfvpli ilgsffmlnl vlgvlsgefa kerervenrq aflkirrqqq lereldgyve
      601 wickaeevil aeertteeek mhimearrra ankrkklksm hnkstdeeee eevedeetpa
      661 gkvklkgfar asylktkikn kgacrafwka ekqfrffirq vvktqtfywv vivlvflntv
      721 cvavehhdqd qyltdflyya efaflglfif emlikvyalg priyfessfn rfdcvviags
      781 ifevvwsslk egsfglsvlr alrllrifkf tkywsslrnl visllnsmrs iisllfllfl
      841 filifallgm qlfggvfnfp egtpaanfnt fpialltvfq iltgedwnev myngiqsqdg
      901 ihggmiysly fiilvlfgny tllnvflaia vdnlanaqel taaeeedaee ekerakeele
      961 keiadlqmat tgsldppqvn icppspqsga pgkgktfpfq enqleagskf kgtndnnnid
     1021 vdedyappre dddedeitgp kpmmpyscmf icsptnpirr aahfivnlry felfimitit
     1081 lssialaaed pveedstknr ilnyfdyaft gvftvemllk iadqgvifhp gaycrdswni
     1141 ldaivvical iafafagsst gqnlstiksl rvlrvlrplk tikrvpklka vfdcvvtslk
     1201 nvfnilivyi lfqfifavis vqlfngkfhy ctdaskltqm eckgeyyvfs dadkppkver
     1261 rqwkhqpfhy dnvmaamltl ftvqtgegwp qvlqnsmdst degkgplpry riemsifyvv
     1321 ffivfpfffv nifvaliiit fqeqgekele egeldknqks cidfaiqakp lqrympknkd
     1381 sfkykvwkiv vstpfeyfim vlivlntvll mmkfynqskt lkntlhymna aftalftiec
     1441 vlkvaafgvr nffknpwhtf dfitvigsii dvlvmetgln ffsvgflrlf raarlikllr
     1501 qgytirillw tfiqsfkalp yvclliamlf fiyaiigmqv fgnimlasds qitrhnnfrt
     1561 ffqglmllfr catgeawqai mmacvkgrpc dplskkdkpe cgsnmayayf vsfiffcsfl
     1621 mlnlfvavim dnfdyltrds silgahhlde firvwaeydp natgyihyte mydmlrnmdp
     1681 plgfgnkcpy rlaykklirm nmpvteegkv nfsttlfali renlsikmrp aeemdqadke
     1741 lkdtirklwp lharkvtdkf mppdselgag rltvgkiygg llilenwrtt rfgqapgags
     1801 ltekasppsp pdsprpsllq rlmgavrnsn trashgslnq deahlddghl qppsrhgslh
     1861 rsslsmerng qssekpwqrs lsflrrgssr rhrppqagpp plpptappap ekgdrpparl
     1921 rpdsialgps pqgsrctspl rrspsprrsa agfsdavsdl vdkvkyetsr rgrararlhg
     1981 deflrdsplr grrgsaeyrs stwlgqrsrs pspsraaerh yggarsaspa asaqslpaqr
     2041 rrlpptpakp srlrldarsm eaginfpsvs qsptlpgars paainfpkvn aspthcprql
     2101 pspllpngyk prrtprrrrs gdtddddwc
//
LOCUS       XP_077504518            2113 aa            linear   INV 25-NOV-2025
DEFINITION  calcium voltage-gated channel subunit cacophony isoform X8
            [Amblyomma americanum].
ACCESSION   XP_077504518
VERSION     XP_077504518.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648392.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2113
                     /product="calcium voltage-gated channel subunit cacophony
                     isoform X8"
                     /calculated_mol_wt=236532
     Region          <56..>154
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     Region          288..577
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          707..938
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1069..1302
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1392..>1541
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1569..1622
                     /region_name="GPHH"
                     /note="Voltage-dependent L-type calcium channel,
                     IQ-associated; pfam16905"
                     /db_xref="CDD:465306"
     Region          1632..1711
                     /region_name="Ca_chan_IQ"
                     /note="Voltage gated calcium channel IQ domain; pfam08763"
                     /db_xref="CDD:462591"
     Region          1886..>2090
                     /region_name="PHA03381"
                     /note="tegument protein VP22; Provisional"
                     /db_xref="CDD:177618"
     CDS             1..2113
                     /gene="cac"
                     /coded_by="XM_077648392.1:64..6405"
                     /db_xref="GeneID:144114565"
ORIGIN      
        1 msgflrfprl vreriserrk rrlrarqrar svavegpgca mtgpttgrpg ggrrgsaplp
       61 vqrraslspq ppgvsaptsl gpspkqsprp svegspkesp psaeqdgpps pggaspaqst
      121 tsalatpqdp aaaaehkqrp tcappggsss lavtaaaapa psprtsrrrr apsrlstelp
      181 agvtlgrrra vtttdhktca llhtrlkgir ledialsals aesggergll rapetsgvlg
      241 ghgsgalrte pgmarrkgld prargpsslf llsednllrr ytkffiewpp leyavlltii
      301 ancivlalee hlpksdrtpl aqklektepy flgifcveal lkivalgfil hrgaylrniw
      361 nimdfvvvvt gfvtyfvped ldldlrtlra irvlrplklv sgipslqvvl ksiikamapl
      421 lqigllvlfa ivifaiigle fysgalhktc ysledaneiv pegeqetpcy qdspinsshp
      481 sgayicdhnt sickegwigp nygitsfdni ffamltvfqc itmegwtail ywtndalgns
      541 fnwvyfvpli ilgsffmlnl vlgvlsgefa kerervenrq aflkirrqqq lereldgyve
      601 wickaeevil aeertteeek mhimearrra ankrkklksm hnkstdeeee eevedeetpa
      661 gkvklkgfar asylktkikn kgacrafwka ekqfrffirq vvktqtfywv vivlvflntv
      721 cvavehhdqd qyltdflyya efaflglfif emlikvyalg priyfessfn rfdcvviags
      781 ifevvwsslk egsfglsvlr alrllrifkf tkywsslrnl visllnsmrs iisllfllfl
      841 filifallgm qlfggvfnfp egtpaanfnt fpialltvfq iltgedwnev myngiqsqdg
      901 ihggmiysly fiilvlfgny tllnvflaia vdnlanaqel taaeeedaee ekerakeele
      961 keiadlqmat tgsldppqvn icppspqsga pgkgktfpfq enqleagskf kgtndnnnid
     1021 vdedyappre dddedeitgp kpmmpyscmf icsptnpirr aahfivnlry felfimitit
     1081 lssialaaed pveedstknr ilnyfdyaft gvftvemllk iadqgvifhp gaycrdswni
     1141 ldaivvical iafafagsst gqnlstiksl rvlrvlrplk tikrvpklka vfdcvvtslk
     1201 nvfnilivyi lfqfifavis vqlfngkfhy ctdaskltqm eckgeyyvfs dadkppkver
     1261 rqwkhqpfhy dnvmaamltl ftvqtgegwp qvlqnsmdst degkgplpry riemsifyvv
     1321 ffivfpfffv nifvaliiit fqeqgekele egeldknqks cidfaiqakp lqrympknkd
     1381 sfkykvwkiv vstpfeyfim vlivlntvll mmkfynqskt lkntlhymna aftalftiec
     1441 vlkvaafgvr nffknpwhtf dfitvigsii dvlvmetgln ffsvgflrlf raarlikllr
     1501 qgytirillw tfiqsfkalp yvclliamlf fiyaiigmqm lnlfvavimd nfdyltrdss
     1561 ilgahhldef irvwaeydpn atgyihytem ydmlrnmdpp lgfgnkcpyr laykklirmn
     1621 mpvteegkvn fsttlfalir enlsikmrpa eemdqadkel kdtirklwpl harkvtdkfm
     1681 ppdselgagr ltvgkiyggl lilenwrttr fgqapgagsl tekasppspp dsprpsllqr
     1741 lmgavrnsnt rashgslnqd eahlddghlq ppsrhgslhr sslsmerngq ssekpwqrsl
     1801 sflrrgssrr hrppqagppp lpptappape kvgagpgagl paavarcmrr tctppggasa
     1861 apppptppgq hpphcarapr epprepprlt lrrraqgdrp parlrpdsia lgpspqgsrc
     1921 tsplrrspsp rrsaagfsda vsdlvdkvky etsrrgrara rlhgdeflrd splrgrrgsa
     1981 eyrsstwlgq rsrspspsra aerhyggars aspaasaqsl paqrrrlppt pakpsrlrld
     2041 arsmeaginf psvsqsptlp garspaainf pkvnaspthc prqlpspllp ngykprrtpr
     2101 rrrsgdtddd dwc
//
LOCUS       XP_077504519            2031 aa            linear   INV 25-NOV-2025
DEFINITION  calcium voltage-gated channel subunit cacophony isoform X9
            [Amblyomma americanum].
ACCESSION   XP_077504519
VERSION     XP_077504519.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648393.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2031
                     /product="calcium voltage-gated channel subunit cacophony
                     isoform X9"
                     /calculated_mol_wt=228253
     Region          <56..>154
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     Region          288..577
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          707..938
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1069..1302
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1392..1641
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1650..1703
                     /region_name="GPHH"
                     /note="Voltage-dependent L-type calcium channel,
                     IQ-associated; pfam16905"
                     /db_xref="CDD:465306"
     Region          1713..1792
                     /region_name="Ca_chan_IQ"
                     /note="Voltage gated calcium channel IQ domain; pfam08763"
                     /db_xref="CDD:462591"
     Region          1816..>2008
                     /region_name="PHA03381"
                     /note="tegument protein VP22; Provisional"
                     /db_xref="CDD:177618"
     CDS             1..2031
                     /gene="cac"
                     /coded_by="XM_077648393.1:64..6159"
                     /db_xref="GeneID:144114565"
ORIGIN      
        1 msgflrfprl vreriserrk rrlrarqrar svavegpgca mtgpttgrpg ggrrgsaplp
       61 vqrraslspq ppgvsaptsl gpspkqsprp svegspkesp psaeqdgpps pggaspaqst
      121 tsalatpqdp aaaaehkqrp tcappggsss lavtaaaapa psprtsrrrr apsrlstelp
      181 agvtlgrrra vtttdhktca llhtrlkgir ledialsals aesggergll rapetsgvlg
      241 ghgsgalrte pgmarrkgld prargpsslf llsednllrr ytkffiewpp leyavlltii
      301 ancivlalee hlpksdrtpl aqklektepy flgifcveal lkivalgfil hrgaylrniw
      361 nimdfvvvvt gfvtyfvped ldldlrtlra irvlrplklv sgipslqvvl ksiikamapl
      421 lqigllvlfa ivifaiigle fysgalhktc ysledaneiv pegeqetpcy qdspinsshp
      481 sgayicdhnt sickegwigp nygitsfdni ffamltvfqc itmegwtail ywtndalgns
      541 fnwvyfvpli ilgsffmlnl vlgvlsgefa kerervenrq aflkirrqqq lereldgyve
      601 wickaeevil aeertteeek mhimearrra ankrkklksm hnkstdeeee eevedeetpa
      661 gkvklkgfar asylktkikn kgacrafwka ekqfrffirq vvktqtfywv vivlvflntv
      721 cvavehhdqd qyltdflyya efaflglfif emlikvyalg priyfessfn rfdcvviags
      781 ifevvwsslk egsfglsvlr alrllrifkf tkywsslrnl visllnsmrs iisllfllfl
      841 filifallgm qlfggvfnfp egtpaanfnt fpialltvfq iltgedwnev myngiqsqdg
      901 ihggmiysly fiilvlfgny tllnvflaia vdnlanaqel taaeeedaee ekerakeele
      961 keiadlqmat tgsldppqvn icppspqsga pgkgktfpfq enqleagskf kgtndnnnid
     1021 vdedyappre dddedeitgp kpmmpyscmf icsptnpirr aahfivnlry felfimitit
     1081 lssialaaed pveedstknr ilnyfdyaft gvftvemllk iadqgvifhp gaycrdswni
     1141 ldaivvical iafafagsst gqnlstiksl rvlrvlrplk tikrvpklka vfdcvvtslk
     1201 nvfnilivyi lfqfifavis vqlfngkfhy ctdaskltqm eckgeyyvfs dadkppkver
     1261 rqwkhqpfhy dnvmaamltl ftvqtgegwp qvlqnsmdst degkgplpry riemsifyvv
     1321 ffivfpfffv nifvaliiit fqeqgekele egeldknqks cidfaiqakp lqrympknkd
     1381 sfkykvwkiv vstpfeyfim vlivlntvll mmkfynqskt lkntlhymna aftalftiec
     1441 vlkvaafgvr nffknpwhtf dfitvigsii dvlvmetgln ffsvgflrlf raarlikllr
     1501 qgytirillw tfiqsfkalp yvclliamlf fiyaiigmqv fgnimlasds qitrhnnfrt
     1561 ffqglmllfr catgeawqai mmacvkgrpc dplskkdkpe cgsnmayayf vsfiffcsfl
     1621 mlnlfvavim dnfdyltrds silgahhlde firvwaeydp natgyihyte mydmlrnmdp
     1681 plgfgnkcpy rlaykklirm nmpvteegkv nfsttlfali renlsikmrp aeemdqadke
     1741 lkdtirklwp lharkvtdkf mppdselgag rltvgkiygg llilenwrtt rfgqapgags
     1801 ltekasppsp pdspgdrppa rlrpdsialg pspqgsrcts plrrspsprr saagfsdavs
     1861 dlvdkvkyet srrgrararl hgdeflrdsp lrgrrgsaey rsstwlgqrs rspspsraae
     1921 rhyggarsas paasaqslpa qrrrlpptpa kpsrlrldar smeaginfps vsqsptlpga
     1981 rspaainfpk vnaspthcpr qlpspllpng ykprrtprrr rsgdtddddw c
//
LOCUS       XP_077504520            1980 aa            linear   INV 25-NOV-2025
DEFINITION  calcium voltage-gated channel subunit cacophony isoform X10
            [Amblyomma americanum].
ACCESSION   XP_077504520
VERSION     XP_077504520.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648394.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1980
                     /product="calcium voltage-gated channel subunit cacophony
                     isoform X10"
                     /calculated_mol_wt=223521
     Region          74..363
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          493..724
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          855..1088
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1178..1427
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1436..1489
                     /region_name="GPHH"
                     /note="Voltage-dependent L-type calcium channel,
                     IQ-associated; pfam16905"
                     /db_xref="CDD:465306"
     Region          1499..1578
                     /region_name="Ca_chan_IQ"
                     /note="Voltage gated calcium channel IQ domain; pfam08763"
                     /db_xref="CDD:462591"
     Region          1753..>1957
                     /region_name="PHA03381"
                     /note="tegument protein VP22; Provisional"
                     /db_xref="CDD:177618"
     CDS             1..1980
                     /gene="cac"
                     /coded_by="XM_077648394.1:87..6029"
                     /db_xref="GeneID:144114565"
ORIGIN      
        1 mlsalsaesg gergllrape tsgvlgghgs galrtepgma rrkgldprar gpsslfllse
       61 dnllrrytkf fiewppleya vlltiianci vlaleehlpk sdrtplaqkl ektepyflgi
      121 fcveallkiv algfilhrga ylrniwnimd fvvvvtgfvt yfvpedldld lrtlrairvl
      181 rplklvsgip slqvvlksii kamapllqig llvlfaivif aiiglefysg alhktcysle
      241 daneivpege qetpcyqdsp insshpsgay icdhntsick egwigpnygi tsfdniffam
      301 ltvfqcitme gwtailywtn dalgnsfnwv yfvpliilgs ffmlnlvlgv lsgefakere
      361 rvenrqaflk irrqqqlere ldgyvewick aeevilaeer tteeekmhim earrraankr
      421 kklksmhnks tdeeeeeeve deetpagkvk lkgfarasyl ktkiknkgac rafwkaekqf
      481 rffirqvvkt qtfywvvivl vflntvcvav ehhdqdqylt dflyyaefaf lglfifemli
      541 kvyalgpriy fessfnrfdc vviagsifev vwsslkegsf glsvlralrl lrifkftkyw
      601 sslrnlvisl lnsmrsiisl lfllflfili fallgmqlfg gvfnfpegtp aanfntfpia
      661 lltvfqiltg edwnevmyng iqsqdgihgg miyslyfiil vlfgnytlln vflaiavdnl
      721 anaqeltaae eedaeeeker akeelekeia dlqmattgsl dppqvnicpp spqsgapgkg
      781 ktfpfqenql eagskfkgtn dnnnidvded yappreddde deitgpkpmm pyscmficsp
      841 tnpirraahf ivnlryfelf imititlssi alaaedpvee dstknrilny fdyaftgvft
      901 vemllkiadq gvifhpgayc rdswnildai vvicaliafa fagsstgqnl stikslrvlr
      961 vlrplktikr vpklkavfdc vvtslknvfn ilivyilfqf ifavisvqlf ngkfhyctda
     1021 skltqmeckg eyyvfsdadk ppkverrqwk hqpfhydnvm aamltlftvq tgegwpqvlq
     1081 nsmdstdegk gplpryriem sifyvvffiv fpfffvnifv aliiitfqeq gekeleegel
     1141 dknqkscidf aiqakplqry mpknkdsfky kvwkivvstp feyfimvliv lntvllmmkf
     1201 ynqsktlknt lhymnaafta lftiecvlkv aafgvrnffk npwhtfdfit vigsiidvlv
     1261 metglnffsv gflrlfraar likllrqgyt irillwtfiq sfkalpyvcl liamlffiya
     1321 iigmqvfgni mlasdsqitr hnnfrtffqg lmllfrcatg eawqaimmac vkgrpcdpls
     1381 kkdkpecgsn mayayfvsfi ffcsflmlnl fvavimdnfd yltrdssilg ahhldefirv
     1441 waeydpnatg yihytemydm lrnmdpplgf gnkcpyrlay kklirmnmpv teegkvnfst
     1501 tlfalirenl sikmrpaeem dqadkelkdt irklwplhar kvtdkfmppd selgagrltv
     1561 gkiyggllil enwrttrfgq apgagsltek asppsppdsp rpsllqrlmg avrnsntras
     1621 hgslnqdeah lddghlqpps rhgslhrssl smerngqsse kpwqrslsfl rrgssrrhrp
     1681 pqagppplpp tappapekvg agpgaglpaa varcmrrtct ppggasaapp pptppgqhpp
     1741 hcaraprepp repprltlrr raqgdrppar lrpdsialgp spqgsrctsp lrrspsprrs
     1801 aagfsdavsd lvdkvkyets rrgrararlh gdeflrdspl rgrrgsaeyr sstwlgqrsr
     1861 spspsraaer hyggarsasp aasaqslpaq rrrlpptpak psrlrldars meaginfpsv
     1921 sqsptlpgar spaainfpkv naspthcprq lpspllpngy kprrtprrrr sgdtddddwc
//
LOCUS       XP_077504521            1942 aa            linear   INV 25-NOV-2025
DEFINITION  calcium voltage-gated channel subunit cacophony isoform X11
            [Amblyomma americanum].
ACCESSION   XP_077504521
VERSION     XP_077504521.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648395.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1942
                     /product="calcium voltage-gated channel subunit cacophony
                     isoform X11"
                     /calculated_mol_wt=219860
     Region          36..325
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          455..686
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          817..1050
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1140..1389
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1398..1451
                     /region_name="GPHH"
                     /note="Voltage-dependent L-type calcium channel,
                     IQ-associated; pfam16905"
                     /db_xref="CDD:465306"
     Region          1461..1540
                     /region_name="Ca_chan_IQ"
                     /note="Voltage gated calcium channel IQ domain; pfam08763"
                     /db_xref="CDD:462591"
     Region          1715..>1919
                     /region_name="PHA03381"
                     /note="tegument protein VP22; Provisional"
                     /db_xref="CDD:177618"
     CDS             1..1942
                     /gene="cac"
                     /coded_by="XM_077648395.1:277..6105"
                     /db_xref="GeneID:144114565"
ORIGIN      
        1 marrkgldpr argpsslfll sednllrryt kffiewpple yavlltiian civlaleehl
       61 pksdrtplaq klektepyfl gifcveallk ivalgfilhr gaylrniwni mdfvvvvtgf
      121 vtyfvpedld ldlrtlrair vlrplklvsg ipslqvvlks iikamapllq igllvlfaiv
      181 ifaiiglefy sgalhktcys ledaneivpe geqetpcyqd spinsshpsg ayicdhntsi
      241 ckegwigpny gitsfdniff amltvfqcit megwtailyw tndalgnsfn wvyfvpliil
      301 gsffmlnlvl gvlsgefake rervenrqaf lkirrqqqle reldgyvewi ckaeevilae
      361 ertteeekmh imearrraan krkklksmhn kstdeeeeee vedeetpagk vklkgfaras
      421 ylktkiknkg acrafwkaek qfrffirqvv ktqtfywvvi vlvflntvcv avehhdqdqy
      481 ltdflyyaef aflglfifem likvyalgpr iyfessfnrf dcvviagsif evvwsslkeg
      541 sfglsvlral rllrifkftk ywsslrnlvi sllnsmrsii sllfllflfi lifallgmql
      601 fggvfnfpeg tpaanfntfp ialltvfqil tgedwnevmy ngiqsqdgih ggmiyslyfi
      661 ilvlfgnytl lnvflaiavd nlanaqelta aeeedaeeek erakeeleke iadlqmattg
      721 sldppqvnic ppspqsgapg kgktfpfqen qleagskfkg tndnnnidvd edyappredd
      781 dedeitgpkp mmpyscmfic sptnpirraa hfivnlryfe lfimititls sialaaedpv
      841 eedstknril nyfdyaftgv ftvemllkia dqgvifhpga ycrdswnild aivvicalia
      901 fafagsstgq nlstikslrv lrvlrplkti krvpklkavf dcvvtslknv fnilivyilf
      961 qfifavisvq lfngkfhyct daskltqmec kgeyyvfsda dkppkverrq wkhqpfhydn
     1021 vmaamltlft vqtgegwpqv lqnsmdstde gkgplpryri emsifyvvff ivfpfffvni
     1081 fvaliiitfq eqgekeleeg eldknqksci dfaiqakplq rympknkdsf kykvwkivvs
     1141 tpfeyfimvl ivlntvllmm kfynqsktlk ntlhymnaaf talftiecvl kvaafgvrnf
     1201 fknpwhtfdf itvigsiidv lvmetglnff svgflrlfra arlikllrqg ytirillwtf
     1261 iqsfkalpyv clliamlffi yaiigmqvfg nimlasdsqi trhnnfrtff qglmllfrca
     1321 tgeawqaimm acvkgrpcdp lskkdkpecg snmayayfvs fiffcsflml nlfvavimdn
     1381 fdyltrdssi lgahhldefi rvwaeydpna tgyihytemy dmlrnmdppl gfgnkcpyrl
     1441 aykklirmnm pvteegkvnf sttlfalire nlsikmrpae emdqadkelk dtirklwplh
     1501 arkvtdkfmp pdselgagrl tvgkiyggll ilenwrttrf gqapgagslt ekasppsppd
     1561 sprpsllqrl mgavrnsntr ashgslnqde ahlddghlqp psrhgslhrs slsmerngqs
     1621 sekpwqrsls flrrgssrrh rppqagpppl pptappapek vgagpgaglp aavarcmrrt
     1681 ctppggasaa pppptppgqh pphcarapre pprepprltl rrraqgdrpp arlrpdsial
     1741 gpspqgsrct splrrspspr rsaagfsdav sdlvdkvkye tsrrgrarar lhgdeflrds
     1801 plrgrrgsae yrsstwlgqr srspspsraa erhyggarsa spaasaqslp aqrrrlpptp
     1861 akpsrlrlda rsmeaginfp svsqsptlpg arspaainfp kvnaspthcp rqlpspllpn
     1921 gykprrtprr rrsgdtdddd wc
//
LOCUS       XP_077504522            1779 aa            linear   INV 25-NOV-2025
DEFINITION  calcium voltage-gated channel subunit cacophony isoform X12
            [Amblyomma americanum].
ACCESSION   XP_077504522
VERSION     XP_077504522.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648396.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1779
                     /product="calcium voltage-gated channel subunit cacophony
                     isoform X12"
                     /calculated_mol_wt=202377
     Region          36..325
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          455..686
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          817..1050
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1140..1389
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          1398..1451
                     /region_name="GPHH"
                     /note="Voltage-dependent L-type calcium channel,
                     IQ-associated; pfam16905"
                     /db_xref="CDD:465306"
     Region          1461..1540
                     /region_name="Ca_chan_IQ"
                     /note="Voltage gated calcium channel IQ domain; pfam08763"
                     /db_xref="CDD:462591"
     Region          1564..>1756
                     /region_name="PHA03381"
                     /note="tegument protein VP22; Provisional"
                     /db_xref="CDD:177618"
     CDS             1..1779
                     /gene="cac"
                     /coded_by="XM_077648396.1:302..5641"
                     /db_xref="GeneID:144114565"
ORIGIN      
        1 marrkgldpr argpsslfll sednllrryt kffiewpple yavlltiian civlaleehl
       61 pksdrtplaq klektepyfl gifcveallk ivalgfilhr gaylrniwni mdfvvvvtgf
      121 vtyfvpedld ldlrtlrair vlrplklvsg ipslqvvlks iikamapllq igllvlfaiv
      181 ifaiiglefy sgalhktcys ledaneivpe geqetpcyqd spinsshpsg ayicdhntsi
      241 ckegwigpny gitsfdniff amltvfqcit megwtailyw tndalgnsfn wvyfvpliil
      301 gsffmlnlvl gvlsgefake rervenrqaf lkirrqqqle reldgyvewi ckaeevilae
      361 ertteeekmh imearrraan krkklksmhn kstdeeeeee vedeetpagk vklkgfaras
      421 ylktkiknkg acrafwkaek qfrffirqvv ktqtfywvvi vlvflntvcv avehhdqdqy
      481 ltdflyyaef aflglfifem likvyalgpr iyfessfnrf dcvviagsif evvwsslkeg
      541 sfglsvlral rllrifkftk ywsslrnlvi sllnsmrsii sllfllflfi lifallgmql
      601 fggvfnfpeg tpaanfntfp ialltvfqil tgedwnevmy ngiqsqdgih ggmiyslyfi
      661 ilvlfgnytl lnvflaiavd nlanaqelta aeeedaeeek erakeeleke iadlqmattg
      721 sldppqvnic ppspqsgapg kgktfpfqen qleagskfkg tndnnnidvd edyappredd
      781 dedeitgpkp mmpyscmfic sptnpirraa hfivnlryfe lfimititls sialaaedpv
      841 eedstknril nyfdyaftgv ftvemllkia dqgvifhpga ycrdswnild aivvicalia
      901 fafagsstgq nlstikslrv lrvlrplkti krvpklkavf dcvvtslknv fnilivyilf
      961 qfifavisvq lfngkfhyct daskltqmec kgeyyvfsda dkppkverrq wkhqpfhydn
     1021 vmaamltlft vqtgegwpqv lqnsmdstde gkgplpryri emsifyvvff ivfpfffvni
     1081 fvaliiitfq eqgekeleeg eldknqksci dfaiqakplq rympknkdsf kykvwkivvs
     1141 tpfeyfimvl ivlntvllmm kfynqsktlk ntlhymnaaf talftiecvl kvaafgvrnf
     1201 fknpwhtfdf itvigsiidv lvmetglnff svgflrlfra arlikllrqg ytirillwtf
     1261 iqsfkalpyv clliamlffi yaiigmqvfg nimlasdsqi trhnnfrtff qglmllfrca
     1321 tgeawqaimm acvkgrpcdp lskkdkpecg snmayayfvs fiffcsflml nlfvavimdn
     1381 fdyltrdssi lgahhldefi rvwaeydpna tgyihytemy dmlrnmdppl gfgnkcpyrl
     1441 aykklirmnm pvteegkvnf sttlfalire nlsikmrpae emdqadkelk dtirklwplh
     1501 arkvtdkfmp pdselgagrl tvgkiyggll ilenwrttrf gqapgagslt ekasppsppd
     1561 spgdrpparl rpdsialgps pqgsrctspl rrspsprrsa agfsdavsdl vdkvkyetsr
     1621 rgrararlhg deflrdsplr grrgsaeyrs stwlgqrsrs pspsraaerh yggarsaspa
     1681 asaqslpaqr rrlpptpakp srlrldarsm eaginfpsvs qsptlpgars paainfpkvn
     1741 aspthcprql pspllpngyk prrtprrrrs gdtddddwc
//
LOCUS       XP_077504523             468 aa            linear   INV 25-NOV-2025
DEFINITION  retinoic acid receptor RXR-alpha-B-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504523
VERSION     XP_077504523.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648397.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..468
                     /product="retinoic acid receptor RXR-alpha-B-like isoform
                     X1"
                     /calculated_mol_wt=51671
     Region          145..221
                     /region_name="NR_DBD_RXR"
                     /note="DNA-binding domain of retinoid X receptor (RXR) is
                     composed of two C4-type zinc fingers; cd06956"
                     /db_xref="CDD:143514"
     Site            order(147,150,164,167,183,189,199,202)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143514"
     Site            order(152,193..194,196,198)
                     /site_type="other"
                     /note="RXR/ECR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(156..159,165..166,168,170,172..173,176,184,196..197,
                     200,203,216..218,220..221)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143514"
     Site            order(178,201,204..205,208..209,213..215,219..221)
                     /site_type="other"
                     /note="RXR/PPAR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(194..195,198)
                     /site_type="other"
                     /note="RXR/RXR homodimer interface DR1 [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            215..220
                     /site_type="other"
                     /note="RXR/RXR homodimer interface DR2 [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(219,221)
                     /site_type="other"
                     /note="RXR/RAR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Region          242..447
                     /region_name="NR_LBD_RXR_like"
                     /note="The ligand binding domain of the retinoid X
                     receptor and Ultraspiracle, members of nuclear receptor
                     superfamily; cd06943"
                     /db_xref="CDD:132741"
     Site            order(275..276,278..279,282,316,320,323,333..334,349,439,
                     442..443)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132741"
     Site            order(284,287,291,296,301..302,304..305,309)
                     /site_type="other"
                     /note="coactivator recognition site [polypeptide binding]"
                     /db_xref="CDD:132741"
     Site            order(355,359,363,380,386,400..401,404..405,408,422..423,
                     426..427,429..431,433..434,437,441)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:132741"
     Site            order(355,359,363,386,388,397,404,408,420,423..424,
                     427..430,433..434,437,441)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:132741"
     CDS             1..468
                     /gene="LOC144114566"
                     /coded_by="XM_077648397.1:132..1538"
                     /db_xref="GeneID:144114566"
ORIGIN      
        1 mlrrpallcs allprgftve esktrgarsr gavafvtfgv pptgervrta aqrgstvlpv
       61 hgrhsrttdw srapglnggt sngvssslpp qpsfllsgyg apsasrspld grqlnngppa
      121 tpqpqqqqqa ttryppnhpl sgskhlcsic gdrasgkhyg vyscegckgf fkrtvrkdls
      181 yacreersci idkrqrnrcq ycryqkclac gmkreavqee rqrtkdrads evestsggap
      241 pemplerile aelrvesqtg tlsesaqqqd pvssicqaad rqlhqlvqwa khiphfeelp
      301 ledrmvllka gwnelliaaf shrsvdvrdg ivlatglvvq rhsahgagvg aifdrvltel
      361 vakmremkmd rtelgclrav vlfnpeakgl rstaqvealr ekvyaaleeh crqqypdqpg
      421 rfaklllrlp alrsiglkcl ehlfffklig dtpidnflls mleapsdp
//
LOCUS       XP_077504524             402 aa            linear   INV 25-NOV-2025
DEFINITION  retinoic acid receptor RXR-alpha-B-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504524
VERSION     XP_077504524.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648398.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..402
                     /product="retinoic acid receptor RXR-alpha-B-like isoform
                     X2"
                     /calculated_mol_wt=44532
     Region          79..155
                     /region_name="NR_DBD_RXR"
                     /note="DNA-binding domain of retinoid X receptor (RXR) is
                     composed of two C4-type zinc fingers; cd06956"
                     /db_xref="CDD:143514"
     Site            order(81,84,98,101,117,123,133,136)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143514"
     Site            order(86,127..128,130,132)
                     /site_type="other"
                     /note="RXR/ECR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(90..93,99..100,102,104,106..107,110,118,130..131,
                     134,137,150..152,154..155)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143514"
     Site            order(112,135,138..139,142..143,147..149,153..155)
                     /site_type="other"
                     /note="RXR/PPAR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(128..129,132)
                     /site_type="other"
                     /note="RXR/RXR homodimer interface DR1 [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            149..154
                     /site_type="other"
                     /note="RXR/RXR homodimer interface DR2 [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(153,155)
                     /site_type="other"
                     /note="RXR/RAR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Region          176..381
                     /region_name="NR_LBD_RXR_like"
                     /note="The ligand binding domain of the retinoid X
                     receptor and Ultraspiracle, members of nuclear receptor
                     superfamily; cd06943"
                     /db_xref="CDD:132741"
     Site            order(209..210,212..213,216,250,254,257,267..268,283,373,
                     376..377)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132741"
     Site            order(218,221,225,230,235..236,238..239,243)
                     /site_type="other"
                     /note="coactivator recognition site [polypeptide binding]"
                     /db_xref="CDD:132741"
     Site            order(289,293,297,314,320,334..335,338..339,342,356..357,
                     360..361,363..365,367..368,371,375)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:132741"
     Site            order(289,293,297,320,322,331,338,342,354,357..358,
                     361..364,367..368,371,375)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:132741"
     CDS             1..402
                     /gene="LOC144114566"
                     /coded_by="XM_077648398.1:137..1345"
                     /db_xref="GeneID:144114566"
ORIGIN      
        1 matqerapgl nggtsngvss slppqpsfll sgygapsasr spldgrqlnn gppatpqpqq
       61 qqqattrypp nhplsgskhl csicgdrasg khygvysceg ckgffkrtvr kdlsyacree
      121 rsciidkrqr nrcqycryqk clacgmkrea vqeerqrtkd radsevests ggappemple
      181 rileaelrve sqtgtlsesa qqqdpvssic qaadrqlhql vqwakhiphf eelpledrmv
      241 llkagwnell iaafshrsvd vrdgivlatg lvvqrhsahg agvgaifdrv ltelvakmre
      301 mkmdrtelgc lravvlfnpe akglrstaqv ealrekvyaa leehcrqqyp dqpgrfakll
      361 lrlpalrsig lkclehlfff kligdtpidn fllsmleaps dp
//
LOCUS       XP_077504525             433 aa            linear   INV 25-NOV-2025
DEFINITION  beta-1,3-galactosyltransferase brn [Amblyomma americanum].
ACCESSION   XP_077504525
VERSION     XP_077504525.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648399.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..433
                     /product="beta-1,3-galactosyltransferase brn"
                     /calculated_mol_wt=49260
     Region          185..389
                     /region_name="Galactosyl_T"
                     /note="Galactosyltransferase; cl21608"
                     /db_xref="CDD:473923"
     CDS             1..433
                     /gene="brn"
                     /coded_by="XM_077648399.1:71..1372"
                     /db_xref="GeneID:144114567"
ORIGIN      
        1 mlrmsallrc svpvipvcli fggvfvvglp asfvivlila vvvfacpswk rltkvlncgd
       61 akstlmtasa dypttpmlrr aklvralqtp rvkwliiasv vvlilettgl ytmcwetpys
      121 rftyplevda rellraleeg rgpgvspins yeryrfkirn erkcaevngt vrllllvksa
      181 lkhvaqrdai rrswgfesrf advvikrvfv lgtgkpemqd evdaeyarhr dlvqadfvda
      241 yynntiktml gfrwafehcr kaefvlfvdd dyfvsvknll kfvrnpwgls aadsqeentp
      301 aafvgdgrlw agfvfprsrp mrhrwskwyl sldeypfsrf ppyvtagafv lsqpalgdly
      361 qvaqytrpfr fddiflgiva lkaglqplhs dafrfwgrpe ssqdfdglva ahgfddpell
      421 vrvweqqksr gqa
//
LOCUS       XP_077504526             488 aa            linear   INV 25-NOV-2025
DEFINITION  putative G-protein coupled receptor CG31760 [Amblyomma americanum].
ACCESSION   XP_077504526
VERSION     XP_077504526.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648400.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..488
                     /product="putative G-protein coupled receptor CG31760"
                     /calculated_mol_wt=55511
     Region          122..371
                     /region_name="7tmC_GPR158-like"
                     /note="orphan GPR158 and similar proteins, member of the
                     class C family of seven-transmembrane G protein-coupled
                     receptors; cd15293"
                     /db_xref="CDD:320420"
     Region          122..147
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320420"
     Site            order(125,128..129,132..133,135..136,170,174,177..178,186,
                     190)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:320420"
     Region          159..180
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320420"
     Site            order(179,191..192,195..196,199,273,278,281..282,285,319,
                     322..323,326,330,342..343,346,349,353)
                     /site_type="other"
                     /note="putative allosteric modulator binding site
                     [chemical binding]"
                     /db_xref="CDD:320420"
     Region          189..213
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320420"
     Region          232..252
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320420"
     Region          274..300
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320420"
     Region          308..331
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320420"
     Region          340..365
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320420"
     Region          <405..>435
                     /region_name="DivIVA"
                     /note="Cell division septum initiation protein DivIVA,
                     interacts with FtsZ and MinD [Cell cycle control, cell
                     division, chromosome partitioning]; COG3599"
                     /db_xref="CDD:442818"
     CDS             1..488
                     /gene="LOC144114568"
                     /coded_by="XM_077648400.1:59..1525"
                     /db_xref="GeneID:144114568"
ORIGIN      
        1 mdidqcdpga pvvpprdrsp aagsfritaf qgthrchnrt sqclfapghg wsrgsyvcqc
       61 rrgfyaatge pffngslves awrekvlfer psydmiyvck pcrpgcnecv ddspclshyn
      121 wafrisllti svicilltmv lagyvyryrk lkvikvaspv flcitligca imycemaaif
      181 pvldvyccva tkwtrhmgfc itystlllkt wrvsltyrvk sahklkltdk qllqwlfpil
      241 lemaiylgtw tissppngiy lkdwhglkfk qceynwwdht maigeflfll wgirvcynvr
      301 naesffneak hiswaiynit ivnlimmlih lvilpsaspd vkylfgfirt qlsttvtvil
      361 ifgpkfyrvi kgqgdmwdnr arargvtasf slngvglmhe etvdlyqene elkeeiqkla
      421 aqiefmkivh memnnrhlkp khggfftsgs qgnstqspvv kamcikfdnt dsagsrispa
      481 aelvserv
//
LOCUS       XP_077504527             582 aa            linear   INV 25-NOV-2025
DEFINITION  sulfhydryl oxidase 1-like [Amblyomma americanum].
ACCESSION   XP_077504527
VERSION     XP_077504527.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648401.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..582
                     /product="sulfhydryl oxidase 1-like"
                     /calculated_mol_wt=64570
     Region          36..142
                     /region_name="Thioredoxin_like"
                     /note="Protein Disulfide Oxidoreductases and Other
                     Proteins with a Thioredoxin fold; cl00388"
                     /db_xref="CDD:469754"
     Region          158..274
                     /region_name="QSOX_Trx1"
                     /note="QSOX Trx-like domain; pfam18108"
                     /db_xref="CDD:465653"
     Region          301..399
                     /region_name="FAD_SOX"
                     /note="Flavin adenine dinucleotide (FAD)-dependent
                     sulfhydryl oxidase; pfam18371"
                     /db_xref="CDD:465728"
     Region          408..496
                     /region_name="Evr1_Alr"
                     /note="Erv1 / Alr family; pfam04777"
                     /db_xref="CDD:461423"
     CDS             1..582
                     /gene="LOC144114569"
                     /coded_by="XM_077648401.1:130..1878"
                     /db_xref="GeneID:144114569"
ORIGIN      
        1 mcgstklsfl lgagilllcr vsfalglasl ydpsmplevl natsfqkvil nkenawlvqf
       61 ynhwcghcik fsplfrafan dvagwkpvis lavidcvdym qtcrtygins yptikffqah
      121 stlhdrgvev etrhnldqlr hqavdlvdsk dayrgprpas wppfeplqgv rsieelwqna
      181 paktntqilv vernasyigk evtlelssrq siyvrrlvvs sdsswlesll phatdasdvr
      241 wadlyalhrn gsttrilrvn eengsrelvl ralqpyirva sstpsstpas tgpiqeisqv
      301 dtsrvyladl nnalayslrq evaghqllnr telaalvhfv dillkyfpgs wsmkqmlqnl
      361 htfvtssggd slrgedlsaf lnkqvelpav gpyegcrgsr pglrgypcal wmlfhsltvs
      421 ayhtsggypl grgtsgadvl qairdyvayf fscrycaahf gamakdldse vhttrdavlw
      481 lwqahnkvna rlagdisedp lhpkqlfppa slcsdcregt qwneqhalsf llhfyspdgl
      541 vpvgvsavvd rearlqlasg gsatrawwsw lvlmlaavlp vp
//
LOCUS       XP_077504528             543 aa            linear   INV 25-NOV-2025
DEFINITION  eukaryotic translation elongation factor, selenocysteine-specific
            [Amblyomma americanum].
ACCESSION   XP_077504528
VERSION     XP_077504528.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648402.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..543
                     /product="eukaryotic translation elongation factor,
                     selenocysteine-specific"
                     /calculated_mol_wt=58603
     Region          8..>381
                     /region_name="SelB"
                     /note="Selenocysteine-specific translation elongation
                     factor SelB [Translation, ribosomal structure and
                     biogenesis]; COG3276"
                     /db_xref="CDD:442507"
     Region          9..177
                     /region_name="SelB_euk"
                     /note="SelB, the dedicated elongation factor for delivery
                     of selenocysteinyl-tRNA to the ribosome; cd01889"
                     /db_xref="CDD:206676"
     Site            14..21
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206676"
     Site            order(15,17,21..22,25,28..29,52..53,81..82,105..106,143,
                     147)
                     /site_type="other"
                     /note="putative GEF interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206676"
     Site            order(17..22,131..132,134,170..172)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206676"
     Site            37..52
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206676"
     Site            48
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206676"
     Site            77..80
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206676"
     Site            79..97
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206676"
     Site            131..134
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206676"
     Site            170..172
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206676"
     CDS             1..543
                     /gene="eEFSec"
                     /coded_by="XM_077648402.1:114..1745"
                     /db_xref="GeneID:144114570"
ORIGIN      
        1 mvvdtfnlnv gvlghvdsgk tslakslstt astacfdknp qsqargitld lgfssfqvea
       61 perlksqcrn lhftlvdcpg haslirtiig gaqiidlmml vidvtkgiqt qtaeclvlge
      121 itcdhmllvl nkidllpdad raklidkttk rlkktlektk fadapiipva anpggaenvd
      181 haatlgldgl vealcqaaql perqdkgplv lsvdhcflvk gqgtvltgti lqgsvavndv
      241 veipslkvsk kvksiqmfrk pvnraiqgdr vgvcvgqlea sllerglvct pghlgaqlfl
      301 larvqpvahf rspcpsgsrf hlsalhatlm arvsflsfpq gsaleevglr wdpemltgpa
      361 aenqeqwall eldqpallap dmlligsrld mdpqgkacrl afhghiqrcy tdrsalaglp
      421 vfkeksrkgl vdrvlsedel vvrslfskgs dlaafhglwv klatgdwgtl agpfgqggkv
      481 rvrlqgepsa qlhallpker gkrpslpadq kpiqvalqfr kylfdasskm vqpkvppgly
      541 rpk
//
LOCUS       XP_077504529             189 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114571 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504529
VERSION     XP_077504529.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648403.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..189
                     /product="uncharacterized protein LOC144114571 isoform X1"
                     /calculated_mol_wt=20758
     Region          8..>81
                     /region_name="Ctr"
                     /note="Ctr copper transporter family; pfam04145"
                     /db_xref="CDD:461194"
     CDS             1..189
                     /gene="LOC144114571"
                     /coded_by="XM_077648403.1:229..798"
                     /db_xref="GeneID:144114571"
ORIGIN      
        1 mevmghqsyf efsthvenll frglsasslg gmlgmclgva afsvlyesit ashhyvtevh
       61 kqslwapsqr srleaspdas qvplltgaft tpstmfsele ykpnaepylq ppsvtarcav
      121 yngiyprsfh qspsttvlpi vyiskhldph klscghgthp nmasnvlqst aeqqvhshnh
      181 lqtsfvkrm
//
LOCUS       XP_077504530             189 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114571 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504530
VERSION     XP_077504530.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648404.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..189
                     /product="uncharacterized protein LOC144114571 isoform X1"
                     /calculated_mol_wt=20758
     Region          8..>81
                     /region_name="Ctr"
                     /note="Ctr copper transporter family; pfam04145"
                     /db_xref="CDD:461194"
     CDS             1..189
                     /gene="LOC144114571"
                     /coded_by="XM_077648404.1:260..829"
                     /db_xref="GeneID:144114571"
ORIGIN      
        1 mevmghqsyf efsthvenll frglsasslg gmlgmclgva afsvlyesit ashhyvtevh
       61 kqslwapsqr srleaspdas qvplltgaft tpstmfsele ykpnaepylq ppsvtarcav
      121 yngiyprsfh qspsttvlpi vyiskhldph klscghgthp nmasnvlqst aeqqvhshnh
      181 lqtsfvkrm
//
LOCUS       XP_077504531             157 aa            linear   INV 25-NOV-2025
DEFINITION  protein SLC31A2-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504531
VERSION     XP_077504531.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648405.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..157
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..157
                     /product="protein SLC31A2-like isoform X2"
                     /calculated_mol_wt=17437
     Region          8..140
                     /region_name="Ctr"
                     /note="Ctr copper transporter family; pfam04145"
                     /db_xref="CDD:461194"
     CDS             1..157
                     /gene="LOC144114571"
                     /coded_by="XM_077648405.1:212..685"
                     /db_xref="GeneID:144114571"
ORIGIN      
        1 mevmghqsyf efsthvenll frglsasslg gmlgmclgva afsvlyesit ashhyvtevh
       61 kqslwapsqr srleaspdas qvnllyghmd aerwgrlkfr llqtllhalq ltlgflvmli
      121 imryngwiai svllasglah hclsrlvmpt rllpvsd
//
LOCUS       XP_077504532             416 aa            linear   INV 25-NOV-2025
DEFINITION  serine protease 33-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504532
VERSION     XP_077504532.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648406.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..416
                     /product="serine protease 33-like isoform X1"
                     /calculated_mol_wt=45189
     Region          <90..143
                     /region_name="TSP1"
                     /note="Thrombospondin type 1 repeats; cl15278"
                     /db_xref="CDD:472794"
     Region          182..410
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cd00190"
                     /db_xref="CDD:238113"
     Site            182
                     /site_type="active"
                     /note="cleavage site [active]"
                     /db_xref="CDD:238113"
     Site            order(223,267,363)
                     /site_type="active"
                     /db_xref="CDD:238113"
     Site            order(357,383,385)
                     /site_type="other"
                     /note="substrate binding sites [chemical binding]"
                     /db_xref="CDD:238113"
     CDS             1..416
                     /gene="LOC144114573"
                     /coded_by="XM_077648406.1:145..1395"
                     /db_xref="GeneID:144114573"
ORIGIN      
        1 mlldviraaa laallllpta aalwgpwsac rrcqqsrrql cprgspdcdg plwlevracp
       61 gsrcpqprlr arsarppgse rnfrvlhhlq slvysnwsgw spcssdcrth rqrqckmplv
      121 cgkavlheda lcyvrgspce arfasggsgg sseedtdsdr gfhldppscg vprspqrpal
      181 riiggqpaar grwpwqvall nrrrepfcgg tlvapgwvlt aahclrrrll vlageyslgr
      241 pegsevtvrv aeaiahpdyd petvdmdlal lrlrsalklg pyvmpaclpe qgdtpppgsl
      301 atilgwgkls krhangsdll hqaqvplvpa secravyady lishnmlcag frrgrvdsca
      361 gdsggpllar dgrgrwtiyg vtsfgegcar qgrfgiyakv anaarwlrri magsrp
//
LOCUS       XP_077504533             362 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114573 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504533
VERSION     XP_077504533.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648407.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..362
                     /product="uncharacterized protein LOC144114573 isoform X2"
                     /calculated_mol_wt=35781
     CDS             1..362
                     /gene="LOC144114573"
                     /coded_by="XM_077648407.1:93..1181"
                     /db_xref="GeneID:144114573"
ORIGIN      
        1 mhalaeqprp iaaqsvsgal llsaavckif prilarsahc yarqrsycgp sgrswiprgs
       61 aaaapprpsg eisggatcig lgnaavpagg gggggargtp plflgsraaa agagpagpql
      121 rhtcnphlsa hlargqgrrh vrqtccwtss erplwlrscs srlrrrsgap grraaaasra
      181 aassargapp tamvrcgsrc apapaaavrs rasglappgl rarsatsacc itcrassipt
      241 glagrpaaat aaptasgnar cpssaakpfs tkmrsatcga aparhaspqe aaagaarrtp
      301 iqtgastwtl paaaclavrr grrcassegs pppgggglgr wrsstggasp sagapwspra
      361 gc
//
LOCUS       XP_077504534             144 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114574 [Amblyomma americanum].
ACCESSION   XP_077504534
VERSION     XP_077504534.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648408.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..144
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..144
                     /product="uncharacterized protein LOC144114574"
                     /calculated_mol_wt=15103
     Region          31..121
                     /region_name="QVR"
                     /note="Sleepless protein; cl25298"
                     /db_xref="CDD:452332"
     CDS             1..144
                     /gene="LOC144114574"
                     /coded_by="XM_077648408.1:126..560"
                     /db_xref="GeneID:144114574"
ORIGIN      
        1 mapqlqpaal aaaaffltla tlpstttaia cyqcnsldnp ecennpldsm vkncsdqskg
       61 skftacrkid envdfevngl pavkrvvrqc avegepdrpc yykagyggrv nvchcfedrc
      121 nsaslpaaaa slaaagvlla lrva
//
LOCUS       XP_077504536             967 aa            linear   INV 25-NOV-2025
DEFINITION  histone-lysine N-methyltransferase G9a [Amblyomma americanum].
ACCESSION   XP_077504536
VERSION     XP_077504536.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648410.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..967
                     /product="histone-lysine N-methyltransferase G9a"
                     /calculated_mol_wt=105883
     Region          221..326
                     /region_name="EHMT_ZBD"
                     /note="Zinc-binding domain of euchromatic histone lysine
                     methyltransferases EHMT1 and EHTM2; cd20905"
                     /db_xref="CDD:411018"
     Region          <455..653
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          485..513
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(488..489,492..494,496..497,501,504,513,515,517,
                     521..522,525..527,529..530,534,537,546,548,550,554..555,
                     558..560,562..563,567,570,579)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          515..546
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          548..579
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          581..612
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          614..645
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          712..942
                     /region_name="SET_EHMT"
                     /note="SET domain (including pre-SET and post-SET domains)
                     found in euchromatic histone-lysine N-methyltransferase
                     EHMT1, EHMT2 and similar proteins; cd10543"
                     /db_xref="CDD:380941"
     Site            order(812..814,831,838,841..842,844,847..852,862,873..877,
                     885,887,900,916,918,921..922,930..934)
                     /site_type="active"
                     /db_xref="CDD:380941"
     Site            order(812..814,848..849,873..877,918,922,930..934)
                     /site_type="other"
                     /note="SAM binding site"
                     /db_xref="CDD:380941"
     Site            order(838,841..842,844,847,850,852,862,921..922,925..926)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:380941"
     Site            order(879,932,934,939)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:380941"
     CDS             1..967
                     /gene="G9a"
                     /coded_by="XM_077648410.1:151..3054"
                     /db_xref="GeneID:144114575"
ORIGIN      
        1 maggrarkgg sngggtaeap arakmsapge aqqvalvrvn tarksfpaac eihkkhrkrf
       61 frgstyglyl sasrrkrrhr kagsdesgts mdgeaavpea gkenscptps aeapaeesas
      121 egnlpngaqp aegtagwghd ssiassvksr qrikrrlmdd hvdvddvtic spssppdgvp
      181 nkkattrkan gstspfcqad lncgrvdvvw dnstqqcqrp cccsnpphps tenpttcqgm
      241 dslqgrvvgc trlpaservq rasvrvpfgs fcgihrhrlr lhhvcphcgl fctqghfkrc
      301 skghlwhaec qknglpkekc phcgcdkaqs vhlealgcpl fqmapqctal gprarmstsr
      361 skppgpavks pnfdtvsvkk dtsdttpsaf pgrkdlddlv rqlrqsatst snrpvlqgaa
      421 gtstllaacr qgdvdrvvhc lagrelgpad fvavhaaaqg gnlvlvhmli qvglpvdhgd
      481 dqlytplmra vdagafpvvq yllkaganph akgedgkscl hlaarfgsve lchiflnakl
      541 pingqddggw tplvwatelk rvdvvrllls rgadpnlrdt eqntalhwsa fsgnvdiswm
      601 yldrgcdpna inergdtplh vaarqdhysv vvlllnykaq ydvtnkqget plkcckdgnt
      661 sthsllsinq mlghmlkskl rvekvlhrdi skgkephais cvngvddtpv psdflylven
      721 cqtaavpldr sitavqsckc qddcssqncv csnisyrcwy dedgclvpef ntvdppmlfe
      781 csraclcwnd crnrvvqkgt tchlqlfrte gkgwgvrtlq diprgafvce yvgevlsdle
      841 adkrgedayl fdlenrdget ycldarhygn vsrfinhlce pnlvpvrvfv dhqdlrfprm
      901 vffssrpiar neelgfdyge kfwmikyklf tcacgspkck yskekigetv lnyqrreeaq
      961 aqastah
//
LOCUS       XP_077504537             967 aa            linear   INV 25-NOV-2025
DEFINITION  histone-lysine N-methyltransferase G9a [Amblyomma americanum].
ACCESSION   XP_077504537
VERSION     XP_077504537.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648411.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..967
                     /product="histone-lysine N-methyltransferase G9a"
                     /calculated_mol_wt=105883
     Region          221..326
                     /region_name="EHMT_ZBD"
                     /note="Zinc-binding domain of euchromatic histone lysine
                     methyltransferases EHMT1 and EHTM2; cd20905"
                     /db_xref="CDD:411018"
     Region          <455..653
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          485..513
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(488..489,492..494,496..497,501,504,513,515,517,
                     521..522,525..527,529..530,534,537,546,548,550,554..555,
                     558..560,562..563,567,570,579)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          515..546
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          548..579
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          581..612
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          614..645
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          712..942
                     /region_name="SET_EHMT"
                     /note="SET domain (including pre-SET and post-SET domains)
                     found in euchromatic histone-lysine N-methyltransferase
                     EHMT1, EHMT2 and similar proteins; cd10543"
                     /db_xref="CDD:380941"
     Site            order(812..814,831,838,841..842,844,847..852,862,873..877,
                     885,887,900,916,918,921..922,930..934)
                     /site_type="active"
                     /db_xref="CDD:380941"
     Site            order(812..814,848..849,873..877,918,922,930..934)
                     /site_type="other"
                     /note="SAM binding site"
                     /db_xref="CDD:380941"
     Site            order(838,841..842,844,847,850,852,862,921..922,925..926)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:380941"
     Site            order(879,932,934,939)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:380941"
     CDS             1..967
                     /gene="G9a"
                     /coded_by="XM_077648411.1:126..3029"
                     /db_xref="GeneID:144114575"
ORIGIN      
        1 maggrarkgg sngggtaeap arakmsapge aqqvalvrvn tarksfpaac eihkkhrkrf
       61 frgstyglyl sasrrkrrhr kagsdesgts mdgeaavpea gkenscptps aeapaeesas
      121 egnlpngaqp aegtagwghd ssiassvksr qrikrrlmdd hvdvddvtic spssppdgvp
      181 nkkattrkan gstspfcqad lncgrvdvvw dnstqqcqrp cccsnpphps tenpttcqgm
      241 dslqgrvvgc trlpaservq rasvrvpfgs fcgihrhrlr lhhvcphcgl fctqghfkrc
      301 skghlwhaec qknglpkekc phcgcdkaqs vhlealgcpl fqmapqctal gprarmstsr
      361 skppgpavks pnfdtvsvkk dtsdttpsaf pgrkdlddlv rqlrqsatst snrpvlqgaa
      421 gtstllaacr qgdvdrvvhc lagrelgpad fvavhaaaqg gnlvlvhmli qvglpvdhgd
      481 dqlytplmra vdagafpvvq yllkaganph akgedgkscl hlaarfgsve lchiflnakl
      541 pingqddggw tplvwatelk rvdvvrllls rgadpnlrdt eqntalhwsa fsgnvdiswm
      601 yldrgcdpna inergdtplh vaarqdhysv vvlllnykaq ydvtnkqget plkcckdgnt
      661 sthsllsinq mlghmlkskl rvekvlhrdi skgkephais cvngvddtpv psdflylven
      721 cqtaavpldr sitavqsckc qddcssqncv csnisyrcwy dedgclvpef ntvdppmlfe
      781 csraclcwnd crnrvvqkgt tchlqlfrte gkgwgvrtlq diprgafvce yvgevlsdle
      841 adkrgedayl fdlenrdget ycldarhygn vsrfinhlce pnlvpvrvfv dhqdlrfprm
      901 vffssrpiar neelgfdyge kfwmikyklf tcacgspkck yskekigetv lnyqrreeaq
      961 aqastah
//
LOCUS       XP_077504538             217 aa            linear   INV 25-NOV-2025
DEFINITION  large ribosomal subunit protein uL1-like [Amblyomma americanum].
ACCESSION   XP_077504538
VERSION     XP_077504538.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648412.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="large ribosomal subunit protein uL1-like"
                     /calculated_mol_wt=24477
     Region          2..217
                     /region_name="PTZ00029"
                     /note="60S ribosomal protein L10a; Provisional"
                     /db_xref="CDD:185405"
     Site            order(26..28,31,33,35,37,162,164,166,210..211,213)
                     /site_type="other"
                     /note="mRNA/rRNA interface [nucleotide binding]"
                     /db_xref="CDD:238235"
     CDS             1..217
                     /gene="LOC144114576"
                     /coded_by="XM_077648412.1:122..775"
                     /db_xref="GeneID:144114576"
ORIGIN      
        1 msskvsrdtl ydcvnavlqe sarkhrkfle tvelqialkn ydpqkdkrfs gtvklkhipr
       61 pkfqvcilgd qqhcdeakan nlacmdteal kklnkskklv kklakkydaf maseslikqi
      121 prllgpglnk agkfptllth nesmmaklde vkatikfqmk kvlclavavg nvkmspdela
      181 qninlainyl vsllkknwqn irslhikttm gppqrly
//
LOCUS       XP_077504539             217 aa            linear   INV 25-NOV-2025
DEFINITION  large ribosomal subunit protein uL1-like [Amblyomma americanum].
ACCESSION   XP_077504539
VERSION     XP_077504539.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648413.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="large ribosomal subunit protein uL1-like"
                     /calculated_mol_wt=24503
     Region          1..217
                     /region_name="Ribosomal_L1"
                     /note="Ribosomal protein L1. The L1 protein, located near
                     the E-site of the ribosome, forms part of the L1 stalk
                     along with 23S rRNA. In bacteria and archaea, L1 functions
                     both as a ribosomal protein that binds rRNA, and as a
                     translation repressor that binds...; cl00322"
                     /db_xref="CDD:469720"
     Site            order(26..28,31,33,35,37,162,164,166,210..211,213)
                     /site_type="other"
                     /note="mRNA/rRNA interface [nucleotide binding]"
                     /db_xref="CDD:238235"
     CDS             1..217
                     /gene="LOC144114577"
                     /coded_by="XM_077648413.1:87..740"
                     /db_xref="GeneID:144114577"
ORIGIN      
        1 mslkvsrdtl ydcvnavlqe sarkhrkfle tvelqialkn ydpqkdkrfs gtvklkhipr
       61 pkfqvcilgd qqhcdeakan nlacmdteal kklnkskklv kklakkydaf maseslikqi
      121 prllgpglnk agkfptllth nesmmaklde vkatikfqmk kvlclavavg nvkmspdela
      181 qninlainyl vsllkknwqn irslhikttm gppqrly
//
LOCUS       XP_077504540             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114578 [Amblyomma americanum].
ACCESSION   XP_077504540
VERSION     XP_077504540.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648414.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144114578"
                     /calculated_mol_wt=17064
     CDS             1..158
                     /gene="LOC144114578"
                     /coded_by="XM_077648414.1:291..767"
                     /db_xref="GeneID:144114578"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdptrrppfa altsvlccqr pasigpcvlp afdcvippdp
       61 fmlktkscme vlqatklrvi fardleqagk lvdgqwysls kasffsktkt iflgswamyl
      121 gtcgqdnagq hasasskkkh etnyrrgtae pggsaepn
//
LOCUS       XP_077504541             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114578 [Amblyomma americanum].
ACCESSION   XP_077504541
VERSION     XP_077504541.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648415.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144114578"
                     /calculated_mol_wt=17064
     CDS             1..158
                     /gene="LOC144114578"
                     /coded_by="XM_077648415.1:291..767"
                     /db_xref="GeneID:144114578"
ORIGIN      
        1 mnrmaavvpa ilrarnssqa rdptrrppfa altsvlccqr pasigpcvlp afdcvippdp
       61 fmlktkscme vlqatklrvi fardleqagk lvdgqwysls kasffsktkt iflgswamyl
      121 gtcgqdnagq hasasskkkh etnyrrgtae pggsaepn
//
LOCUS       XP_077504542             479 aa            linear   INV 25-NOV-2025
DEFINITION  diacylglycerol O-acyltransferase isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504542
VERSION     XP_077504542.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648416.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..479
                     /product="diacylglycerol O-acyltransferase isoform X1"
                     /calculated_mol_wt=55844
     Region          40..465
                     /region_name="MBOAT"
                     /note="membrane-bound O-acyltransferase family; cl00738"
                     /db_xref="CDD:445070"
     CDS             1..479
                     /gene="mdy"
                     /coded_by="XM_077648416.1:188..1627"
                     /db_xref="GeneID:144114579"
ORIGIN      
        1 mahkdgttat tngvrirrtl slanalqiqe yeakerrlap dlpihkpcds lfsssrgwtn
       61 yrglfnfcii llvlsnsrva lenllkygil vdpgkwvryl lldpriwppv yvtlglnvci
      121 lcslftekll aagrvsdnva rnvqvlnfss illippvvvf vwdcnpvass lalgcvtvlf
      181 lklvsyhmvn lwcrqqrasr khhrrrsssg tdqithgang rttnghltkf vtypdnlnly
      241 diyyfifvpt lcyelnfprs srirkrflfr rflesllllq lilalaqqwi vpimenslkp
      301 fqemnfpaml erllklavpn iliwllifyw ffhstlntfa ellrfadref yrdwwnadtv
      361 qyfwqnwnip vhrwclrhly kplvasgmsk gkacvmvfll saffheylvs vplkmfrvwa
      421 ffgmlaqvpf afvidncfrn rynnlgnmav wisliigqpl ailmyyhdyy iinygtrtl
//
LOCUS       XP_077504543             482 aa            linear   INV 25-NOV-2025
DEFINITION  diacylglycerol O-acyltransferase isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504543
VERSION     XP_077504543.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648417.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..482
                     /product="diacylglycerol O-acyltransferase isoform X2"
                     /calculated_mol_wt=55796
     Region          63..468
                     /region_name="MBOAT"
                     /note="membrane-bound O-acyltransferase family; cl00738"
                     /db_xref="CDD:445070"
     CDS             1..482
                     /gene="mdy"
                     /coded_by="XM_077648417.1:165..1613"
                     /db_xref="GeneID:144114579"
ORIGIN      
        1 mesddklspv aksaraasva gaaasprmad gseqvtkdvp fallrrihkp cdslfsssrg
       61 wtnyrglfnf ciillvlsns rvalenllky gilvdpgkwv ryllldpriw ppvyvtlgln
      121 vcilcslfte kllaagrvsd nvarnvqvln fssillippv vvfvwdcnpv asslalgcvt
      181 vlflklvsyh mvnlwcrqqr asrkhhrrrs ssgtdqithg angrttnghl tkfvtypdnl
      241 nlydiyyfif vptlcyelnf prssrirkrf lfrrflesll llqlilalaq qwivpimens
      301 lkpfqemnfp amlerllkla vpniliwlli fywffhstln tfaellrfad refyrdwwna
      361 dtvqyfwqnw nipvhrwclr hlykplvasg mskgkacvmv fllsaffhey lvsvplkmfr
      421 vwaffgmlaq vpfafvidnc frnrynnlgn mavwisliig qplailmyyh dyyiinygtr
      481 tl
//
LOCUS       XP_077504544             139 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114581 [Amblyomma americanum].
ACCESSION   XP_077504544
VERSION     XP_077504544.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648418.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..139
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..139
                     /product="uncharacterized protein LOC144114581"
                     /calculated_mol_wt=14777
     CDS             1..139
                     /gene="LOC144114581"
                     /coded_by="XM_077648418.1:478..897"
                     /db_xref="GeneID:144114581"
ORIGIN      
        1 msclgflqsg vatgtlrhpw lsgtpeqgcr tggkaereag wqpasfyseh phesalsgpq
       61 qprdglpekr qcsttgseps qhrgarpkyg lssrltaqfp eqfstspevq vggrgpvpgg
      121 saegpqiprq rrtllpayi
//
LOCUS       XP_077504545             139 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114582 [Amblyomma americanum].
ACCESSION   XP_077504545
VERSION     XP_077504545.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648419.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..139
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..139
                     /product="uncharacterized protein LOC144114582"
                     /calculated_mol_wt=14796
     CDS             1..139
                     /gene="LOC144114582"
                     /coded_by="XM_077648419.1:497..916"
                     /db_xref="GeneID:144114582"
ORIGIN      
        1 msclgflqsg vatgtlrhpw lsgtpeqgcr tggkaervar wqpanlyseh phestlsgpq
       61 qpldglpekr qcsttgseps qhrgarpkyg lssrltaqfp eqfstspevr vggrgpvpgg
      121 saegpqiprq prtllpayi
//
LOCUS       XP_077504546             139 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114582 [Amblyomma americanum].
ACCESSION   XP_077504546
VERSION     XP_077504546.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648420.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..139
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..139
                     /product="uncharacterized protein LOC144114582"
                     /calculated_mol_wt=14796
     CDS             1..139
                     /gene="LOC144114582"
                     /coded_by="XM_077648420.1:493..912"
                     /db_xref="GeneID:144114582"
ORIGIN      
        1 msclgflqsg vatgtlrhpw lsgtpeqgcr tggkaervar wqpanlyseh phestlsgpq
       61 qpldglpekr qcsttgseps qhrgarpkyg lssrltaqfp eqfstspevr vggrgpvpgg
      121 saegpqiprq prtllpayi
//
LOCUS       XP_077504547             214 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114583 [Amblyomma americanum].
ACCESSION   XP_077504547
VERSION     XP_077504547.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648421.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..214
                     /product="uncharacterized protein LOC144114583"
                     /calculated_mol_wt=24622
     Region          23..43
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(23,26,39,43)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          <48..210
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          51..71
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(51,54,67,71)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          79..99
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(79,82,95,99)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(84,86,88,90..91,94..95,98,112,114,118..119,122..123,
                     126,140,142,144,146..147,150..151,154)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          107..127
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          135..155
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          163..183
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          191..207
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..214
                     /gene="LOC144114583"
                     /coded_by="XM_077648421.1:280..924"
                     /db_xref="GeneID:144114583"
ORIGIN      
        1 mestpsgmgn hsgkptgnkl hecpncsmrf karsklvvhl rvhtgdrpyq cndcgirfsl
       61 ianlkrhqrt htgdrpyqct ncgssfarka tlkihqrtht gerpyhcdhc nssfsnqknl
      121 vrhqrihtge rpyrcdhcsr tfaqkqslvl hirthtgekp fqcqlcpmaf vmrlglvrhe
      181 rshkgekpfq cdlcqkafsr rpalkhhkea ihki
//
LOCUS       XP_077504548             116 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114586 [Amblyomma americanum].
ACCESSION   XP_077504548
VERSION     XP_077504548.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648422.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..116
                     /product="uncharacterized protein LOC144114586"
                     /calculated_mol_wt=12450
     CDS             1..116
                     /gene="LOC144114586"
                     /coded_by="XM_077648422.1:80..430"
                     /db_xref="GeneID:144114586"
ORIGIN      
        1 mrahsldrsn hemdclrnds vqprgqkkrr randnialls sclssntigv daiwgarhgn
       61 mpgccasnsm vclyfdesas gngslgklgn rcghgdtpap laivnsrarl pnwrqs
//
LOCUS       XP_077504549             891 aa            linear   INV 25-NOV-2025
DEFINITION  DBB domain-containing protein stumps isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504549
VERSION     XP_077504549.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648423.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..891
                     /product="DBB domain-containing protein stumps isoform X1"
                     /calculated_mol_wt=96765
     Region          234..369
                     /region_name="DBB"
                     /note="Dof, BCAP, and BANK (DBB) motif; pfam14545"
                     /db_xref="CDD:464205"
     CDS             1..891
                     /gene="stumps"
                     /coded_by="XM_077648423.1:16..2691"
                     /db_xref="GeneID:144114587"
ORIGIN      
        1 msfasffpri rrgaarsctr rpgdsgrqrr ttprpkvfag alptraaang tdgpwrpste
       61 spptlgpada pvpllrlgma advstlfcnd grewgsylad cfrqqaltvt qealdaislp
      121 lpqdrakalg aakvsllivs pdllellerr gaelfsvgrl leserglaml cgvephelgs
      181 slraafpnfs swpllearnq drefvirlve tareiverse nsmrgtaaas pqfrlcprkv
      241 hqgnakvfam lnepveqsqk lqlffessgm helpvkwrnp hvlqftvpet flqvskvvnv
      301 qlrcdgvllg vrqlkcesqm sqlhallqsa tspyemlclt lglatlhdvd talatnlrrn
      361 lptrgfhlla pqqlaapekk rseellptll hfaahyglhe lslallqcpg astccqilnc
      421 kghspaqlas naghhrlaat inefqtrais svteeeapvp pparvqsnpg rgspprmhye
      481 nvtvgghyvs pgaarsapvc npsyglaslv pppaapappa dndqeaeyls mlpqvatpke
      541 rssvsqgdqe eektevnsts eqnnigapdm ifsntqedlv riiemykkgm pfsqieklfd
      601 nwkksnestk stedshlqal relykqkqra aptgkqsfsl sdlrqiitgk gnrrgsrgrd
      661 iqhttlpnma nkkqddsteg crrvstlsav stsssgssss sdrlstfssm saydsgahsd
      721 sceegrpppr pprlpaprnp ktamhqyyaf apgsgptdrn aplhslagdl tgptipevst
      781 nsvpsssslh gnlstgakpa tnpsagiyyd ipqvprpvdq rhnplldydf pvaalrriap
      841 pppacpepdd grhyydipkw gaagappptp snkqqvrlsi pdippppvpd d
//
LOCUS       XP_077504550             855 aa            linear   INV 25-NOV-2025
DEFINITION  DBB domain-containing protein stumps isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504550
VERSION     XP_077504550.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648424.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..855
                     /product="DBB domain-containing protein stumps isoform X2"
                     /calculated_mol_wt=93064
     Region          198..333
                     /region_name="DBB"
                     /note="Dof, BCAP, and BANK (DBB) motif; pfam14545"
                     /db_xref="CDD:464205"
     CDS             1..855
                     /gene="stumps"
                     /coded_by="XM_077648424.1:49..2616"
                     /db_xref="GeneID:144114587"
ORIGIN      
        1 mncllcgrat rpwsicaamd lrldrhrhsg padapvpllr lgmaadvstl fcndgrewgs
       61 yladcfrqqa ltvtqealda islplpqdra kalgaakvsl livspdllel lerrgaelfs
      121 vgrlleserg lamlcgveph elgsslraaf pnfsswplle arnqdrefvi rlvetareiv
      181 ersensmrgt aaaspqfrlc prkvhqgnak vfamlnepve qsqklqlffe ssgmhelpvk
      241 wrnphvlqft vpetflqvsk vvnvqlrcdg vllgvrqlkc esqmsqlhal lqsatspyem
      301 lcltlglatl hdvdtalatn lrrnlptrgf hllapqqlaa pekkrseell ptllhfaahy
      361 glhelslall qcpgastccq ilnckghspa qlasnaghhr laatinefqt raissvteee
      421 apvppparvq snpgrgsppr mhyenvtvgg hyvspgaars apvcnpsygl aslvpppaap
      481 appadndqea eylsmlpqva tpkerssvsq gdqeeektev nstseqnnig apdmifsntq
      541 edlvriiemy kkgmpfsqie klfdnwkksn estkstedsh lqalrelykq kqraaptgkq
      601 sfslsdlrqi itgkgnrrgs rgrdiqhttl pnmankkqdd stegcrrvst lsavstsssg
      661 sssssdrlst fssmsaydsg ahsdsceegr ppprpprlpa prnpktamhq yyafapgsgp
      721 tdrnaplhsl agdltgptip evstnsvpss sslhgnlstg akpatnpsag iyydipqvpr
      781 pvdqrhnpll dydfpvaalr riappppacp epddgrhyyd ipkwgaagap pptpsnkqqv
      841 rlsipdippp pvpdd
//
LOCUS       XP_077504551             849 aa            linear   INV 25-NOV-2025
DEFINITION  DBB domain-containing protein stumps isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504551
VERSION     XP_077504551.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648425.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..849
                     /product="DBB domain-containing protein stumps isoform X3"
                     /calculated_mol_wt=92143
     Region          192..327
                     /region_name="DBB"
                     /note="Dof, BCAP, and BANK (DBB) motif; pfam14545"
                     /db_xref="CDD:464205"
     CDS             1..849
                     /gene="stumps"
                     /coded_by="XM_077648425.1:360..2909"
                     /db_xref="GeneID:144114587"
ORIGIN      
        1 maptplthpa hsadgtahrw tahgpadapv pllrlgmaad vstlfcndgr ewgsyladcf
       61 rqqaltvtqe aldaislplp qdrakalgaa kvsllivspd llellerrga elfsvgrlle
      121 serglamlcg vephelgssl raafpnfssw pllearnqdr efvirlveta reiversens
      181 mrgtaaaspq frlcprkvhq gnakvfamln epveqsqklq lffessgmhe lpvkwrnphv
      241 lqftvpetfl qvskvvnvql rcdgvllgvr qlkcesqmsq lhallqsats pyemlcltlg
      301 latlhdvdta latnlrrnlp trgfhllapq qlaapekkrs eellptllhf aahyglhels
      361 lallqcpgas tccqilnckg hspaqlasna ghhrlaatin efqtraissv teeeapvppp
      421 arvqsnpgrg spprmhyenv tvgghyvspg aarsapvcnp syglaslvpp paapappadn
      481 dqeaeylsml pqvatpkers svsqgdqeee ktevnstseq nnigapdmif sntqedlvri
      541 iemykkgmpf sqieklfdnw kksnestkst edshlqalre lykqkqraap tgkqsfslsd
      601 lrqiitgkgn rrgsrgrdiq httlpnmank kqddstegcr rvstlsavst sssgsssssd
      661 rlstfssmsa ydsgahsdsc eegrppprpp rlpaprnpkt amhqyyafap gsgptdrnap
      721 lhslagdltg ptipevstns vpsssslhgn lstgakpatn psagiyydip qvprpvdqrh
      781 nplldydfpv aalrriappp pacpepddgr hyydipkwga agappptpsn kqqvrlsipd
      841 ippppvpdd
//
LOCUS       XP_077504552             842 aa            linear   INV 25-NOV-2025
DEFINITION  DBB domain-containing protein stumps isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077504552
VERSION     XP_077504552.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648426.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..842
                     /product="DBB domain-containing protein stumps isoform X4"
                     /calculated_mol_wt=91429
     Region          185..320
                     /region_name="DBB"
                     /note="Dof, BCAP, and BANK (DBB) motif; pfam14545"
                     /db_xref="CDD:464205"
     CDS             1..842
                     /gene="stumps"
                     /coded_by="XM_077648426.1:36..2564"
                     /db_xref="GeneID:144114587"
ORIGIN      
        1 mvpphhhctp hgidgngpad apvpllrlgm aadvstlfcn dgrewgsyla dcfrqqaltv
       61 tqealdaisl plpqdrakal gaakvslliv spdlleller rgaelfsvgr lleserglam
      121 lcgvephelg sslraafpnf sswpllearn qdrefvirlv etareivers ensmrgtaaa
      181 spqfrlcprk vhqgnakvfa mlnepveqsq klqlffessg mhelpvkwrn phvlqftvpe
      241 tflqvskvvn vqlrcdgvll gvrqlkcesq msqlhallqs atspyemlcl tlglatlhdv
      301 dtalatnlrr nlptrgfhll apqqlaapek krseellptl lhfaahyglh elslallqcp
      361 gastccqiln ckghspaqla snaghhrlaa tinefqtrai ssvteeeapv ppparvqsnp
      421 grgspprmhy envtvgghyv spgaarsapv cnpsyglasl vpppaapapp adndqeaeyl
      481 smlpqvatpk erssvsqgdq eeektevnst seqnnigapd mifsntqedl vriiemykkg
      541 mpfsqieklf dnwkksnest kstedshlqa lrelykqkqr aaptgkqsfs lsdlrqiitg
      601 kgnrrgsrgr diqhttlpnm ankkqddste gcrrvstlsa vstsssgsss ssdrlstfss
      661 msaydsgahs dsceegrppp rpprlpaprn pktamhqyya fapgsgptdr naplhslagd
      721 ltgptipevs tnsvpssssl hgnlstgakp atnpsagiyy dipqvprpvd qrhnplldyd
      781 fpvaalrria ppppacpepd dgrhyydipk wgaagapppt psnkqqvrls ipdippppvp
      841 dd
//
LOCUS       XP_077504553             847 aa            linear   INV 25-NOV-2025
DEFINITION  DBB domain-containing protein stumps isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077504553
VERSION     XP_077504553.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648427.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..847
                     /product="DBB domain-containing protein stumps isoform X5"
                     /calculated_mol_wt=92319
     Region          190..325
                     /region_name="DBB"
                     /note="Dof, BCAP, and BANK (DBB) motif; pfam14545"
                     /db_xref="CDD:464205"
     CDS             1..847
                     /gene="stumps"
                     /coded_by="XM_077648427.1:147..2690"
                     /db_xref="GeneID:144114587"
ORIGIN      
        1 mreprrmtws qfkgwaaclr kgpadapvpl lrlgmaadvs tlfcndgrew gsyladcfrq
       61 qaltvtqeal daislplpqd rakalgaakv sllivspdll ellerrgael fsvgrllese
      121 rglamlcgve phelgsslra afpnfsswpl learnqdref virlvetare iversensmr
      181 gtaaaspqfr lcprkvhqgn akvfamlnep veqsqklqlf fessgmhelp vkwrnphvlq
      241 ftvpetflqv skvvnvqlrc dgvllgvrql kcesqmsqlh allqsatspy emlcltlgla
      301 tlhdvdtala tnlrrnlptr gfhllapqql aapekkrsee llptllhfaa hyglhelsla
      361 llqcpgastc cqilnckghs paqlasnagh hrlaatinef qtraissvte eeapvpppar
      421 vqsnpgrgsp prmhyenvtv gghyvspgaa rsapvcnpsy glaslvpppa apappadndq
      481 eaeylsmlpq vatpkerssv sqgdqeeekt evnstseqnn igapdmifsn tqedlvriie
      541 mykkgmpfsq ieklfdnwkk snestksted shlqalrely kqkqraaptg kqsfslsdlr
      601 qiitgkgnrr gsrgrdiqht tlpnmankkq ddstegcrrv stlsavstss sgsssssdrl
      661 stfssmsayd sgahsdscee grppprpprl paprnpktam hqyyafapgs gptdrnaplh
      721 slagdltgpt ipevstnsvp sssslhgnls tgakpatnps agiyydipqv prpvdqrhnp
      781 lldydfpvaa lrriappppa cpepddgrhy ydipkwgaag appptpsnkq qvrlsipdip
      841 pppvpdd
//
LOCUS       XP_077504554             970 aa            linear   INV 25-NOV-2025
DEFINITION  DBB domain-containing protein stumps isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077504554
VERSION     XP_077504554.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648428.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..970
                     /product="DBB domain-containing protein stumps isoform X6"
                     /calculated_mol_wt=104287
     Region          313..448
                     /region_name="DBB"
                     /note="Dof, BCAP, and BANK (DBB) motif; pfam14545"
                     /db_xref="CDD:464205"
     CDS             1..970
                     /gene="stumps"
                     /coded_by="XM_077648428.1:152..3064"
                     /db_xref="GeneID:144114587"
ORIGIN      
        1 mttadavrsg grssdpmhpa giaaglaqls drcrelrqra rsllflppcl dcyarpddvc
       61 akpgqgspsa dlsspgrgqs svaagswgag aaaasrplps altgelqrpr raviaepvag
      121 lqrcsashva qtalgtacts ctahgpadap vpllrlgmaa dvstlfcndg rewgsyladc
      181 frqqaltvtq ealdaislpl pqdrakalga akvsllivsp dllellerrg aelfsvgrll
      241 eserglamlc gvephelgss lraafpnfss wpllearnqd refvirlvet areiversen
      301 smrgtaaasp qfrlcprkvh qgnakvfaml nepveqsqkl qlffessgmh elpvkwrnph
      361 vlqftvpetf lqvskvvnvq lrcdgvllgv rqlkcesqms qlhallqsat spyemlcltl
      421 glatlhdvdt alatnlrrnl ptrgfhllap qqlaapekkr seellptllh faahyglhel
      481 slallqcpga stccqilnck ghspaqlasn aghhrlaati nefqtraiss vteeeapvpp
      541 parvqsnpgr gspprmhyen vtvgghyvsp gaarsapvcn psyglaslvp ppaapappad
      601 ndqeaeylsm lpqvatpker ssvsqgdqee ektevnstse qnnigapdmi fsntqedlvr
      661 iiemykkgmp fsqieklfdn wkksnestks tedshlqalr elykqkqraa ptgkqsfsls
      721 dlrqiitgkg nrrgsrgrdi qhttlpnman kkqddstegc rrvstlsavs tsssgsssss
      781 drlstfssms aydsgahsds ceegrppprp prlpaprnpk tamhqyyafa pgsgptdrna
      841 plhslagdlt gptipevstn svpsssslhg nlstgakpat npsagiyydi pqvprpvdqr
      901 hnplldydfp vaalrriapp ppacpepddg rhyydipkwg aagappptps nkqqvrlsip
      961 dippppvpdd
//
LOCUS       XP_077504556             813 aa            linear   INV 25-NOV-2025
DEFINITION  DBB domain-containing protein stumps isoform X7 [Amblyomma
            americanum].
ACCESSION   XP_077504556
VERSION     XP_077504556.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648430.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..813
                     /product="DBB domain-containing protein stumps isoform X7"
                     /calculated_mol_wt=88440
     Region          156..291
                     /region_name="DBB"
                     /note="Dof, BCAP, and BANK (DBB) motif; pfam14545"
                     /db_xref="CDD:464205"
     CDS             1..813
                     /gene="stumps"
                     /coded_by="XM_077648430.1:145..2586"
                     /db_xref="GeneID:144114587"
ORIGIN      
        1 maadvstlfc ndgrewgsyl adcfrqqalt vtqealdais lplpqdraka lgaakvslli
       61 vspdllelle rrgaelfsvg rllesergla mlcgvephel gsslraafpn fsswpllear
      121 nqdrefvirl vetareiver sensmrgtaa aspqfrlcpr kvhqgnakvf amlnepveqs
      181 qklqlffess gmhelpvkwr nphvlqftvp etflqvskvv nvqlrcdgvl lgvrqlkces
      241 qmsqlhallq satspyemlc ltlglatlhd vdtalatnlr rnlptrgfhl lapqqlaape
      301 kkrseellpt llhfaahygl helslallqc pgastccqil nckghspaql asnaghhrla
      361 atinefqtra issvteeeap vppparvqsn pgrgspprmh yenvtvgghy vspgaarsap
      421 vcnpsyglas lvpppaapap padndqeaey lsmlpqvatp kerssvsqgd qeeektevns
      481 tseqnnigap dmifsntqed lvriiemykk gmpfsqiekl fdnwkksnes tkstedshlq
      541 alrelykqkq raaptgkqsf slsdlrqiit gkgnrrgsrg rdiqhttlpn mankkqddst
      601 egcrrvstls avstsssgss sssdrlstfs smsaydsgah sdsceegrpp prpprlpapr
      661 npktamhqyy afapgsgptd rnaplhslag dltgptipev stnsvpssss lhgnlstgak
      721 patnpsagiy ydipqvprpv dqrhnplldy dfpvaalrri appppacpep ddgrhyydip
      781 kwgaagappp tpsnkqqvrl sipdippppv pdd
//
LOCUS       XP_077504557             265 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114589 [Amblyomma americanum].
ACCESSION   XP_077504557
VERSION     XP_077504557.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648431.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..265
                     /product="uncharacterized protein LOC144114589"
                     /calculated_mol_wt=30149
     Region          170..254
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     CDS             1..265
                     /gene="LOC144114589"
                     /coded_by="XM_077648431.1:1584..2381"
                     /db_xref="GeneID:144114589"
ORIGIN      
        1 mpccalqldl ltgsavsyal tptgrvqrpd illlcqwild awkaipanls plrdiwwscw
       61 vpsmfrapps srdaspqrfa pedepaaqqa srrlqgdepe fgtvqdqprq rkdaatstat
      121 spkmstpiil qqpqvpptfn gssgedpeew ldqyervasf nnwddaskfq hvffalessa
      181 rtwyenress lttwelfkke lmkvftsvvr keravrlles riqlpnepvr gyveeikrlf
      241 rradtgmtek kkvtvkghty pasfv
//
LOCUS       XP_077504558             412 aa            linear   INV 25-NOV-2025
DEFINITION  tachykinin-like peptides receptor 99D [Amblyomma americanum].
ACCESSION   XP_077504558
VERSION     XP_077504558.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648432.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..412
                     /product="tachykinin-like peptides receptor 99D"
                     /calculated_mol_wt=46386
     Region          43..335
                     /region_name="7tmA_TACR"
                     /note="neurokinin receptors (or tachykinin receptors),
                     member of the class A family of seven-transmembrane G
                     protein-coupled receptors; cd15390"
                     /db_xref="CDD:320512"
     Region          44..70
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320512"
     Region          77..103
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320512"
     Site            order(99,102..103,115..120,122..123,126,169,171..175,212,
                     215..217,219..221,223..224,280,283..284,286..287,290,
                     302..303,305..307,310,313..314)
                     /site_type="other"
                     /note="putative peptide ligand binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:320512"
     Region          115..145
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320512"
     Region          156..177
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320512"
     Region          212..241
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320512"
     Region          260..290
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320512"
     Region          303..328
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320512"
     CDS             1..412
                     /gene="LOC144114590"
                     /coded_by="XM_077648432.1:1461..2699"
                     /db_xref="GeneID:144114590"
ORIGIN      
        1 mdlylndsis dsngtapegv smvnlssedm tenvyilpww gqaawillfg smilvatggn
       61 liviwivlah krmrtvtnyf ivnlsladtm vstlnvifnf ifmlngnwpf gtayckvsnf
      121 iaivsvaasv ftlmaicidr ymaimrplrp rmsrattlni aigiwvassl lslpnilhst
      181 tkeefydngd krvicyilwp dsddgvtash deyvynvvil ivtymipmts maftyfrvgr
      241 elwgsqsige ctakqieaik skrkivkmmi vvvvifavcw lpyhvyflvm hhhpeinqst
      301 yiqhvylaiy wlamsnsmyn piiycwmnsr fregfkrvfc clafndreta qlrqhmaatr
      361 yscsepyadt rvtfngtata vplhtltetm sagnltagpg saakfrgvgs dv
//
LOCUS       XP_077504559             147 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114591 [Amblyomma americanum].
ACCESSION   XP_077504559
VERSION     XP_077504559.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648433.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..147
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..147
                     /product="uncharacterized protein LOC144114591"
                     /calculated_mol_wt=13526
     CDS             1..147
                     /gene="LOC144114591"
                     /coded_by="XM_077648433.1:15..458"
                     /db_xref="GeneID:144114591"
ORIGIN      
        1 mphscadlrk qklvalfvcg fagvciagvv ggygggyglg ygggfgggfg glgyggglgy
       61 ggglghglgl vktvpgpsfl vktvhnvnrv shgstlfghy ghgggyggvg lgggyggalv
      121 gggyggglgg gfggglgggy akvavkg
//
LOCUS       XP_077504560             851 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin carboxyl-terminal hydrolase 20-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504560
VERSION     XP_077504560.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648434.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..851
                     /product="ubiquitin carboxyl-terminal hydrolase 20-like
                     isoform X1"
                     /calculated_mol_wt=94908
     Region          31..92
                     /region_name="zf-UBP"
                     /note="Zn-finger in ubiquitin-hydrolases and other
                     protein; pfam02148"
                     /db_xref="CDD:460464"
     Region          142..606
                     /region_name="UCH"
                     /note="Ubiquitin carboxyl-terminal hydrolase; pfam00443"
                     /db_xref="CDD:425685"
     Site            order(146,151,567,585)
                     /site_type="active"
                     /db_xref="CDD:239139"
     Region          636..709
                     /region_name="DUSP"
                     /note="DUSP domain; cl12116"
                     /db_xref="CDD:416436"
     Region          744..817
                     /region_name="DUSP"
                     /note="DUSP domain; pfam06337"
                     /db_xref="CDD:399383"
     CDS             1..851
                     /gene="LOC144114592"
                     /coded_by="XM_077648434.1:295..2850"
                     /db_xref="GeneID:144114592"
ORIGIN      
        1 magplsacph vttvgdftke diarkqeqwr cescgasgps lwaclyrdcl yvgcgeafkd
       61 hstqhfqscc ehsvtlnlst lrvwcylces evflennrpp llgaspriss qaaghtrrap
      121 apapdsddea drenslkprg ltglqnlgnt cymnaalqal sncpqltnff ldcggfvqcd
      181 kkpglsksym rlvremwhkr rpsyvvpsgi aygikmvypv frgysqqdaq eflrcfldql
      241 heelkapvga farlplgrpr sptaessess lsedeedfet cdsglsseks scgddqdlll
      301 rshggqqedm rmiveekagg dwptldssap ggskghrark maaahrlcsi vsdvfdgril
      361 ssvqcltcdt vsttretfqd lslpipsrdh lavlhaaqgs ntsgvpgaht ratlehqqgw
      421 lswilgrmfs wvfgwlplwp tlrvprvkke vgklwlwgpg valqdclsaf fsadelkgdn
      481 myscdkcckl rngikysrvl qlpevlcihl krfrhevmfs skissyvsfp legldmapfl
      541 htecprtats ydlmavichh gtagfghytt yalnhlndqw yefddqyvts vepqvvqnce
      601 ayvlfyrkss eemlnrrtrt yelmersrse pcllsffisk qwvnrfntfa dpgpidngdf
      661 lcihggvqpa kahiaeelcv rlsqsvweyl hdsfgggpac thlytcptct serealerrr
      721 kaeldtfial nrefqqsqds pgvvyavsma wfkqweafvr rkdteapgpi dnwcicaapr
      781 gsgslqqslr pgsdygslse amwqflqgsy gggpqvvlht rtanppsqqa ppgvpgvarq
      841 tsnedtpees m
//
LOCUS       XP_077504561             851 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin carboxyl-terminal hydrolase 20-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504561
VERSION     XP_077504561.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648435.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..851
                     /product="ubiquitin carboxyl-terminal hydrolase 20-like
                     isoform X1"
                     /calculated_mol_wt=94908
     Region          31..92
                     /region_name="zf-UBP"
                     /note="Zn-finger in ubiquitin-hydrolases and other
                     protein; pfam02148"
                     /db_xref="CDD:460464"
     Region          142..606
                     /region_name="UCH"
                     /note="Ubiquitin carboxyl-terminal hydrolase; pfam00443"
                     /db_xref="CDD:425685"
     Site            order(146,151,567,585)
                     /site_type="active"
                     /db_xref="CDD:239139"
     Region          636..709
                     /region_name="DUSP"
                     /note="DUSP domain; cl12116"
                     /db_xref="CDD:416436"
     Region          744..817
                     /region_name="DUSP"
                     /note="DUSP domain; pfam06337"
                     /db_xref="CDD:399383"
     CDS             1..851
                     /gene="LOC144114592"
                     /coded_by="XM_077648435.1:484..3039"
                     /db_xref="GeneID:144114592"
ORIGIN      
        1 magplsacph vttvgdftke diarkqeqwr cescgasgps lwaclyrdcl yvgcgeafkd
       61 hstqhfqscc ehsvtlnlst lrvwcylces evflennrpp llgaspriss qaaghtrrap
      121 apapdsddea drenslkprg ltglqnlgnt cymnaalqal sncpqltnff ldcggfvqcd
      181 kkpglsksym rlvremwhkr rpsyvvpsgi aygikmvypv frgysqqdaq eflrcfldql
      241 heelkapvga farlplgrpr sptaessess lsedeedfet cdsglsseks scgddqdlll
      301 rshggqqedm rmiveekagg dwptldssap ggskghrark maaahrlcsi vsdvfdgril
      361 ssvqcltcdt vsttretfqd lslpipsrdh lavlhaaqgs ntsgvpgaht ratlehqqgw
      421 lswilgrmfs wvfgwlplwp tlrvprvkke vgklwlwgpg valqdclsaf fsadelkgdn
      481 myscdkcckl rngikysrvl qlpevlcihl krfrhevmfs skissyvsfp legldmapfl
      541 htecprtats ydlmavichh gtagfghytt yalnhlndqw yefddqyvts vepqvvqnce
      601 ayvlfyrkss eemlnrrtrt yelmersrse pcllsffisk qwvnrfntfa dpgpidngdf
      661 lcihggvqpa kahiaeelcv rlsqsvweyl hdsfgggpac thlytcptct serealerrr
      721 kaeldtfial nrefqqsqds pgvvyavsma wfkqweafvr rkdteapgpi dnwcicaapr
      781 gsgslqqslr pgsdygslse amwqflqgsy gggpqvvlht rtanppsqqa ppgvpgvarq
      841 tsnedtpees m
//
LOCUS       XP_077504562             850 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin carboxyl-terminal hydrolase 20-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504562
VERSION     XP_077504562.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648436.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..850
                     /product="ubiquitin carboxyl-terminal hydrolase 20-like
                     isoform X2"
                     /calculated_mol_wt=94779
     Region          31..92
                     /region_name="zf-UBP"
                     /note="Zn-finger in ubiquitin-hydrolases and other
                     protein; pfam02148"
                     /db_xref="CDD:460464"
     Region          142..605
                     /region_name="UCH"
                     /note="Ubiquitin carboxyl-terminal hydrolase; pfam00443"
                     /db_xref="CDD:425685"
     Site            order(146,151,566,584)
                     /site_type="active"
                     /db_xref="CDD:239139"
     Region          635..708
                     /region_name="DUSP"
                     /note="DUSP domain; cl12116"
                     /db_xref="CDD:416436"
     Region          743..816
                     /region_name="DUSP"
                     /note="DUSP domain; pfam06337"
                     /db_xref="CDD:399383"
     CDS             1..850
                     /gene="LOC144114592"
                     /coded_by="XM_077648436.1:296..2848"
                     /db_xref="GeneID:144114592"
ORIGIN      
        1 magplsacph vttvgdftke diarkqeqwr cescgasgps lwaclyrdcl yvgcgeafkd
       61 hstqhfqscc ehsvtlnlst lrvwcylces evflennrpp llgaspriss qaaghtrrap
      121 apapdsddea drenslkprg ltglqnlgnt cymnaalqal sncpqltnff ldcggfvqcd
      181 kkpglsksym rlvremwhkr rpsyvvpsgi aygikmvypv frgysqqdaq eflrcfldql
      241 heelkapvga farlplgrpr sptaessess lsedeedfet cdsglsseks scgddqdlll
      301 rshggqedmr miveekaggd wptldssapg gskghrarkm aaahrlcsiv sdvfdgrils
      361 svqcltcdtv sttretfqdl slpipsrdhl avlhaaqgsn tsgvpgahtr atlehqqgwl
      421 swilgrmfsw vfgwlplwpt lrvprvkkev gklwlwgpgv alqdclsaff sadelkgdnm
      481 yscdkccklr ngikysrvlq lpevlcihlk rfrhevmfss kissyvsfpl egldmapflh
      541 tecprtatsy dlmavichhg tagfghytty alnhlndqwy efddqyvtsv epqvvqncea
      601 yvlfyrksse emlnrrtrty elmersrsep cllsffiskq wvnrfntfad pgpidngdfl
      661 cihggvqpak ahiaeelcvr lsqsvweylh dsfgggpact hlytcptcts erealerrrk
      721 aeldtfialn refqqsqdsp gvvyavsmaw fkqweafvrr kdteapgpid nwcicaaprg
      781 sgslqqslrp gsdygslsea mwqflqgsyg ggpqvvlhtr tanppsqqap pgvpgvarqt
      841 snedtpeesm
//
LOCUS       XP_077504563             848 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin carboxyl-terminal hydrolase 20-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504563
VERSION     XP_077504563.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648437.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..848
                     /product="ubiquitin carboxyl-terminal hydrolase 20-like
                     isoform X3"
                     /calculated_mol_wt=94654
     Region          31..92
                     /region_name="zf-UBP"
                     /note="Zn-finger in ubiquitin-hydrolases and other
                     protein; pfam02148"
                     /db_xref="CDD:460464"
     Region          142..606
                     /region_name="UCH"
                     /note="Ubiquitin carboxyl-terminal hydrolase; pfam00443"
                     /db_xref="CDD:425685"
     Site            order(146,151,567,585)
                     /site_type="active"
                     /db_xref="CDD:239139"
     Region          636..709
                     /region_name="DUSP"
                     /note="DUSP domain; cl12116"
                     /db_xref="CDD:416436"
     Region          744..817
                     /region_name="DUSP"
                     /note="DUSP domain; pfam06337"
                     /db_xref="CDD:399383"
     CDS             1..848
                     /gene="LOC144114592"
                     /coded_by="XM_077648437.1:295..2841"
                     /db_xref="GeneID:144114592"
ORIGIN      
        1 magplsacph vttvgdftke diarkqeqwr cescgasgps lwaclyrdcl yvgcgeafkd
       61 hstqhfqscc ehsvtlnlst lrvwcylces evflennrpp llgaspriss qaaghtrrap
      121 apapdsddea drenslkprg ltglqnlgnt cymnaalqal sncpqltnff ldcggfvqcd
      181 kkpglsksym rlvremwhkr rpsyvvpsgi aygikmvypv frgysqqdaq eflrcfldql
      241 heelkapvga farlplgrpr sptaessess lsedeedfet cdsglsseks scgddqdlll
      301 rshggqqedm rmiveekagg dwptldssap ggskghrark maaahrlcsi vsdvfdgril
      361 ssvqcltcdt vsttretfqd lslpipsrdh lavlhaaqgs ntsgvpgaht ratlehqqgw
      421 lswilgrmfs wvfgwlplwp tlrvprvkke vgklwlwgpg valqdclsaf fsadelkgdn
      481 myscdkcckl rngikysrvl qlpevlcihl krfrhevmfs skissyvsfp legldmapfl
      541 htecprtats ydlmavichh gtagfghytt yalnhlndqw yefddqyvts vepqvvqnce
      601 ayvlfyrkss eemlnrrtrt yelmersrse pcllsffisk qwvnrfntfa dpgpidngdf
      661 lcihggvqpa kahiaeelcv rlsqsvweyl hdsfgggpac thlytcptct serealerrr
      721 kaeldtfial nrefqqsqds pgvvyavsma wfkqweafvr rkdteapgpi dnwcicaapr
      781 gsgslqqslr pgsdygslse amwqflqgsy gggpqvvlht rtanppsqqa ppgvarqtsn
      841 edtpeesm
//
LOCUS       XP_077504564             831 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin carboxyl-terminal hydrolase 20-like isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077504564
VERSION     XP_077504564.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648438.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..831
                     /product="ubiquitin carboxyl-terminal hydrolase 20-like
                     isoform X4"
                     /calculated_mol_wt=92510
     Region          31..92
                     /region_name="zf-UBP"
                     /note="Zn-finger in ubiquitin-hydrolases and other
                     protein; pfam02148"
                     /db_xref="CDD:460464"
     Region          142..586
                     /region_name="UCH"
                     /note="Ubiquitin carboxyl-terminal hydrolase; pfam00443"
                     /db_xref="CDD:425685"
     Site            order(146,151,547,565)
                     /site_type="active"
                     /db_xref="CDD:239139"
     Region          616..689
                     /region_name="DUSP"
                     /note="DUSP domain; cl12116"
                     /db_xref="CDD:416436"
     Region          724..797
                     /region_name="DUSP"
                     /note="DUSP domain; pfam06337"
                     /db_xref="CDD:399383"
     CDS             1..831
                     /gene="LOC144114592"
                     /coded_by="XM_077648438.1:293..2788"
                     /db_xref="GeneID:144114592"
ORIGIN      
        1 magplsacph vttvgdftke diarkqeqwr cescgasgps lwaclyrdcl yvgcgeafkd
       61 hstqhfqscc ehsvtlnlst lrvwcylces evflennrpp llgaspriss qaaghtrrap
      121 apapdsddea drenslkprg ltglqnlgnt cymnaalqal sncpqltnff ldcggfvqcd
      181 kkpglsksym rlvremwhkr rpsyvvpsgi aygikmvypv frgysqqdaq eflrcfldql
      241 heelkapvga farlplgrpr sptaessess lsedeedfet cdsglsseks scgddqdlll
      301 rshggqqedm rmiveekagg dwptldssap ggskghrark maaahrlcsi vsdvfdgril
      361 ssvqcltcdt vsttretfqd lslpipsrdh lavlhaaqgs ntsgvpgaht ratlehqqgw
      421 lswilgrmfs wvfgwlwgpg valqdclsaf fsadelkgdn myscdkcckl rngikysrvl
      481 qlpevlcihl krfrhevmfs skissyvsfp legldmapfl htecprtats ydlmavichh
      541 gtagfghytt yalnhlndqw yefddqyvts vepqvvqnce ayvlfyrkss eemlnrrtrt
      601 yelmersrse pcllsffisk qwvnrfntfa dpgpidngdf lcihggvqpa kahiaeelcv
      661 rlsqsvweyl hdsfgggpac thlytcptct serealerrr kaeldtfial nrefqqsqds
      721 pgvvyavsma wfkqweafvr rkdteapgpi dnwcicaapr gsgslqqslr pgsdygslse
      781 amwqflqgsy gggpqvvlht rtanppsqqa ppgvpgvarq tsnedtpees m
//
LOCUS       XP_077504565             831 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin carboxyl-terminal hydrolase 20-like isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077504565
VERSION     XP_077504565.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648439.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..831
                     /product="ubiquitin carboxyl-terminal hydrolase 20-like
                     isoform X4"
                     /calculated_mol_wt=92510
     Region          31..92
                     /region_name="zf-UBP"
                     /note="Zn-finger in ubiquitin-hydrolases and other
                     protein; pfam02148"
                     /db_xref="CDD:460464"
     Region          142..586
                     /region_name="UCH"
                     /note="Ubiquitin carboxyl-terminal hydrolase; pfam00443"
                     /db_xref="CDD:425685"
     Site            order(146,151,547,565)
                     /site_type="active"
                     /db_xref="CDD:239139"
     Region          616..689
                     /region_name="DUSP"
                     /note="DUSP domain; cl12116"
                     /db_xref="CDD:416436"
     Region          724..797
                     /region_name="DUSP"
                     /note="DUSP domain; pfam06337"
                     /db_xref="CDD:399383"
     CDS             1..831
                     /gene="LOC144114592"
                     /coded_by="XM_077648439.1:482..2977"
                     /db_xref="GeneID:144114592"
ORIGIN      
        1 magplsacph vttvgdftke diarkqeqwr cescgasgps lwaclyrdcl yvgcgeafkd
       61 hstqhfqscc ehsvtlnlst lrvwcylces evflennrpp llgaspriss qaaghtrrap
      121 apapdsddea drenslkprg ltglqnlgnt cymnaalqal sncpqltnff ldcggfvqcd
      181 kkpglsksym rlvremwhkr rpsyvvpsgi aygikmvypv frgysqqdaq eflrcfldql
      241 heelkapvga farlplgrpr sptaessess lsedeedfet cdsglsseks scgddqdlll
      301 rshggqqedm rmiveekagg dwptldssap ggskghrark maaahrlcsi vsdvfdgril
      361 ssvqcltcdt vsttretfqd lslpipsrdh lavlhaaqgs ntsgvpgaht ratlehqqgw
      421 lswilgrmfs wvfgwlwgpg valqdclsaf fsadelkgdn myscdkcckl rngikysrvl
      481 qlpevlcihl krfrhevmfs skissyvsfp legldmapfl htecprtats ydlmavichh
      541 gtagfghytt yalnhlndqw yefddqyvts vepqvvqnce ayvlfyrkss eemlnrrtrt
      601 yelmersrse pcllsffisk qwvnrfntfa dpgpidngdf lcihggvqpa kahiaeelcv
      661 rlsqsvweyl hdsfgggpac thlytcptct serealerrr kaeldtfial nrefqqsqds
      721 pgvvyavsma wfkqweafvr rkdteapgpi dnwcicaapr gsgslqqslr pgsdygslse
      781 amwqflqgsy gggpqvvlht rtanppsqqa ppgvpgvarq tsnedtpees m
//
LOCUS       XP_077504566             787 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin carboxyl-terminal hydrolase 20-like isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077504566
VERSION     XP_077504566.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648440.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..787
                     /product="ubiquitin carboxyl-terminal hydrolase 20-like
                     isoform X5"
                     /calculated_mol_wt=87892
     Region          31..92
                     /region_name="zf-UBP"
                     /note="Zn-finger in ubiquitin-hydrolases and other
                     protein; pfam02148"
                     /db_xref="CDD:460464"
     Region          142..606
                     /region_name="UCH"
                     /note="Ubiquitin carboxyl-terminal hydrolase; pfam00443"
                     /db_xref="CDD:425685"
     Site            order(146,151,567,585)
                     /site_type="active"
                     /db_xref="CDD:239139"
     Region          636..709
                     /region_name="DUSP"
                     /note="DUSP domain; cl12116"
                     /db_xref="CDD:416436"
     CDS             1..787
                     /gene="LOC144114592"
                     /coded_by="XM_077648440.1:296..2659"
                     /db_xref="GeneID:144114592"
ORIGIN      
        1 magplsacph vttvgdftke diarkqeqwr cescgasgps lwaclyrdcl yvgcgeafkd
       61 hstqhfqscc ehsvtlnlst lrvwcylces evflennrpp llgaspriss qaaghtrrap
      121 apapdsddea drenslkprg ltglqnlgnt cymnaalqal sncpqltnff ldcggfvqcd
      181 kkpglsksym rlvremwhkr rpsyvvpsgi aygikmvypv frgysqqdaq eflrcfldql
      241 heelkapvga farlplgrpr sptaessess lsedeedfet cdsglsseks scgddqdlll
      301 rshggqqedm rmiveekagg dwptldssap ggskghrark maaahrlcsi vsdvfdgril
      361 ssvqcltcdt vsttretfqd lslpipsrdh lavlhaaqgs ntsgvpgaht ratlehqqgw
      421 lswilgrmfs wvfgwlplwp tlrvprvkke vgklwlwgpg valqdclsaf fsadelkgdn
      481 myscdkcckl rngikysrvl qlpevlcihl krfrhevmfs skissyvsfp legldmapfl
      541 htecprtats ydlmavichh gtagfghytt yalnhlndqw yefddqyvts vepqvvqnce
      601 ayvlfyrkss eemlnrrtrt yelmersrse pcllsffisk qwvnrfntfa dpgpidngdf
      661 lcihggvqpa kahiaeelcv rlsqsvweyl hdsfgggpac thlytcptct serealerrr
      721 kaeldtfiav skaasrkteq rvsaepgqpr rclcskhglv qavgslcatq ghrgtwshrq
      781 lvhlcss
//
LOCUS       XP_077504567             828 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin carboxyl-terminal hydrolase 20-like isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077504567
VERSION     XP_077504567.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648441.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..828
                     /product="ubiquitin carboxyl-terminal hydrolase 20-like
                     isoform X6"
                     /calculated_mol_wt=92256
     Region          31..92
                     /region_name="zf-UBP"
                     /note="Zn-finger in ubiquitin-hydrolases and other
                     protein; pfam02148"
                     /db_xref="CDD:460464"
     Region          142..586
                     /region_name="UCH"
                     /note="Ubiquitin carboxyl-terminal hydrolase; pfam00443"
                     /db_xref="CDD:425685"
     Site            order(146,151,547,565)
                     /site_type="active"
                     /db_xref="CDD:239139"
     Region          616..689
                     /region_name="DUSP"
                     /note="DUSP domain; cl12116"
                     /db_xref="CDD:416436"
     Region          724..797
                     /region_name="DUSP"
                     /note="DUSP domain; pfam06337"
                     /db_xref="CDD:399383"
     CDS             1..828
                     /gene="LOC144114592"
                     /coded_by="XM_077648441.1:294..2780"
                     /db_xref="GeneID:144114592"
ORIGIN      
        1 magplsacph vttvgdftke diarkqeqwr cescgasgps lwaclyrdcl yvgcgeafkd
       61 hstqhfqscc ehsvtlnlst lrvwcylces evflennrpp llgaspriss qaaghtrrap
      121 apapdsddea drenslkprg ltglqnlgnt cymnaalqal sncpqltnff ldcggfvqcd
      181 kkpglsksym rlvremwhkr rpsyvvpsgi aygikmvypv frgysqqdaq eflrcfldql
      241 heelkapvga farlplgrpr sptaessess lsedeedfet cdsglsseks scgddqdlll
      301 rshggqqedm rmiveekagg dwptldssap ggskghrark maaahrlcsi vsdvfdgril
      361 ssvqcltcdt vsttretfqd lslpipsrdh lavlhaaqgs ntsgvpgaht ratlehqqgw
      421 lswilgrmfs wvfgwlwgpg valqdclsaf fsadelkgdn myscdkcckl rngikysrvl
      481 qlpevlcihl krfrhevmfs skissyvsfp legldmapfl htecprtats ydlmavichh
      541 gtagfghytt yalnhlndqw yefddqyvts vepqvvqnce ayvlfyrkss eemlnrrtrt
      601 yelmersrse pcllsffisk qwvnrfntfa dpgpidngdf lcihggvqpa kahiaeelcv
      661 rlsqsvweyl hdsfgggpac thlytcptct serealerrr kaeldtfial nrefqqsqds
      721 pgvvyavsma wfkqweafvr rkdteapgpi dnwcicaapr gsgslqqslr pgsdygslse
      781 amwqflqgsy gggpqvvlht rtanppsqqa ppgvarqtsn edtpeesm
//
LOCUS       XP_077504569             533 aa            linear   INV 25-NOV-2025
DEFINITION  high-affinity choline transporter 1-like [Amblyomma americanum].
ACCESSION   XP_077504569
VERSION     XP_077504569.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648443.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..533
                     /product="high-affinity choline transporter 1-like"
                     /calculated_mol_wt=56605
     Region          8..494
                     /region_name="SLC5sbd_CHT"
                     /note="Na(+)- and Cl(-)-dependent choline cotransporter
                     CHT and related proteins; solute-binding domain; cd11474"
                     /db_xref="CDD:271368"
     Site            order(68,71,345,348..349)
                     /site_type="other"
                     /note="Na binding site [ion binding]"
                     /db_xref="CDD:271368"
     CDS             1..533
                     /gene="LOC144114595"
                     /coded_by="XM_077648443.1:179..1780"
                     /db_xref="GeneID:144114595"
ORIGIN      
        1 malnvaglat mvvlyvvifa vgllgarkkk qqqkqqhsqv atdtsdsrll mnggrnfglv
       61 istftiiatw idggflngtv evvttkglvw cqavfgysls llmggliiag pmwragyvtm
      121 mdplqetfgv tmasllflps lageicwcgs ilnalgstvs vvtglndavs igvsaavavl
      181 ytitggvysv aytdvvqltf ifvglcicvp ftllneytnp iwsvpkeell gsvatseips
      241 yvdnllllif ggipwqdyfq rilstksefr akalsyvgfv gtlvmavppv ligvavkatr
      301 wnetafpsga dptdekyapm mlslalqylt ptavscvglg viaaavmssa dscllscgsi
      361 aarniyvpll rpkasdtevv wvlrlcvvtv aaasaamavs vhsvydlwal sadlvyvvll
      421 pqllgavflg rrwcnatgsl aafalglalr vlggepvlgi pavmhyplyd gnrqlfpfkt
      481 vtmllsgvtl lavsrvadwv tgskraarle appspaavkl skermlssnd lwk
//
LOCUS       XP_077504570             533 aa            linear   INV 25-NOV-2025
DEFINITION  high-affinity choline transporter 1-like [Amblyomma americanum].
ACCESSION   XP_077504570
VERSION     XP_077504570.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648444.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..533
                     /product="high-affinity choline transporter 1-like"
                     /calculated_mol_wt=56605
     Region          8..494
                     /region_name="SLC5sbd_CHT"
                     /note="Na(+)- and Cl(-)-dependent choline cotransporter
                     CHT and related proteins; solute-binding domain; cd11474"
                     /db_xref="CDD:271368"
     Site            order(68,71,345,348..349)
                     /site_type="other"
                     /note="Na binding site [ion binding]"
                     /db_xref="CDD:271368"
     CDS             1..533
                     /gene="LOC144114595"
                     /coded_by="XM_077648444.1:144..1745"
                     /db_xref="GeneID:144114595"
ORIGIN      
        1 malnvaglat mvvlyvvifa vgllgarkkk qqqkqqhsqv atdtsdsrll mnggrnfglv
       61 istftiiatw idggflngtv evvttkglvw cqavfgysls llmggliiag pmwragyvtm
      121 mdplqetfgv tmasllflps lageicwcgs ilnalgstvs vvtglndavs igvsaavavl
      181 ytitggvysv aytdvvqltf ifvglcicvp ftllneytnp iwsvpkeell gsvatseips
      241 yvdnllllif ggipwqdyfq rilstksefr akalsyvgfv gtlvmavppv ligvavkatr
      301 wnetafpsga dptdekyapm mlslalqylt ptavscvglg viaaavmssa dscllscgsi
      361 aarniyvpll rpkasdtevv wvlrlcvvtv aaasaamavs vhsvydlwal sadlvyvvll
      421 pqllgavflg rrwcnatgsl aafalglalr vlggepvlgi pavmhyplyd gnrqlfpfkt
      481 vtmllsgvtl lavsrvadwv tgskraarle appspaavkl skermlssnd lwk
//
LOCUS       XP_077504571             684 aa            linear   INV 25-NOV-2025
DEFINITION  sirtuin 1 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504571
VERSION     XP_077504571.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648445.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..684
                     /product="sirtuin 1 isoform X1"
                     /calculated_mol_wt=74187
     Region          <199..241
                     /region_name="DUF592"
                     /note="Protein of unknown function (DUF592); pfam04574"
                     /db_xref="CDD:368003"
     Region          235..475
                     /region_name="SIRT1"
                     /note="Eukaryotic group (class1) which includes human
                     sirtuins SIRT1-3 and yeast Hst1-4; and are members of the
                     SIR2 family of proteins, silent information regulator 2
                     (Sir2) enzymes which catalyze NAD+-dependent
                     protein/histone deacetylation. Sir2 proteins...; cd01408"
                     /db_xref="CDD:238699"
     Site            order(244,246..247,254..255,308,326..327,329,344,426,431,
                     452..453,467)
                     /site_type="other"
                     /note="NAD+ binding site [chemical binding]"
                     /db_xref="CDD:238699"
     Site            order(328,344,398,400..404,430..432)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:238699"
     Site            order(352,355,376,379)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:238699"
     CDS             1..684
                     /gene="Sirt1"
                     /coded_by="XM_077648445.1:140..2194"
                     /db_xref="GeneID:144114597"
ORIGIN      
        1 madrreassa spaykrprle ndsnllglsi pndvvmppke fkrqrfasda anevtsssda
       61 nsemcngdsg fhelssttgg dmvtadgvcp dndhlvtagv arevdssghl flgalgavdv
      121 apkddicddd adevastisq lsglsdlsdl agaewkptse gpmgwvqrqm slgadprsll
      181 erllpdgavi pssldrltlw kvlismlsee epprrtklah vntlddvvsl lrtcqrvlvl
      241 tgagvsvscg ipdfrsrngi yarlskdfpa lpdpqamfdi hyfrkdprpf fkfakeiypg
      301 qftpsashrf ikllednnkl lrnytqnidt leqtcgirnv itchgsfata sctrchhkvd
      361 cnmikedifs qripvcpkcs peeveasgem avmkpdivff geglsqefhq amsrdksqcd
      421 llivmgsslk vrpvalipss ippevpqili nreslkhvtf dvellgdcdv iikelcnrlg
      481 wdvdsdeepv aplteltslp pqvsaksvse irepaeqrgn vaadengntp ssstaigeev
      541 mpdstcgemt pavesqsdsl dcsseclwkp riresvasrl peghylftsp styifsgaev
      601 yvdsedsdsp gasftgeeee dvesdsessh stfssdeees pahtsglesp sqgntmdcdp
      661 aeqgpstgpt smplvgnlge fych
//
LOCUS       XP_077504572             683 aa            linear   INV 25-NOV-2025
DEFINITION  sirtuin 1 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504572
VERSION     XP_077504572.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648446.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..683
                     /product="sirtuin 1 isoform X2"
                     /calculated_mol_wt=74116
     Region          <198..240
                     /region_name="DUF592"
                     /note="Protein of unknown function (DUF592); pfam04574"
                     /db_xref="CDD:368003"
     Region          234..474
                     /region_name="SIRT1"
                     /note="Eukaryotic group (class1) which includes human
                     sirtuins SIRT1-3 and yeast Hst1-4; and are members of the
                     SIR2 family of proteins, silent information regulator 2
                     (Sir2) enzymes which catalyze NAD+-dependent
                     protein/histone deacetylation. Sir2 proteins...; cd01408"
                     /db_xref="CDD:238699"
     Site            order(243,245..246,253..254,307,325..326,328,343,425,430,
                     451..452,466)
                     /site_type="other"
                     /note="NAD+ binding site [chemical binding]"
                     /db_xref="CDD:238699"
     Site            order(327,343,397,399..403,429..431)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:238699"
     Site            order(351,354,375,378)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:238699"
     CDS             1..683
                     /gene="Sirt1"
                     /coded_by="XM_077648446.1:140..2191"
                     /db_xref="GeneID:144114597"
ORIGIN      
        1 madrreassa spaykrprle ndsnllglsi pndvvmppke fkrqrfasda anevtsssda
       61 nsemcngdsg fhelssttgg dmvtadgvcp dndhlvtgva revdssghlf lgalgavdva
      121 pkddicddda devastisql sglsdlsdla gaewkptseg pmgwvqrqms lgadprslle
      181 rllpdgavip ssldrltlwk vlismlseee pprrtklahv ntlddvvsll rtcqrvlvlt
      241 gagvsvscgi pdfrsrngiy arlskdfpal pdpqamfdih yfrkdprpff kfakeiypgq
      301 ftpsashrfi kllednnkll rnytqnidtl eqtcgirnvi tchgsfatas ctrchhkvdc
      361 nmikedifsq ripvcpkcsp eeveasgema vmkpdivffg eglsqefhqa msrdksqcdl
      421 livmgsslkv rpvalipssi ppevpqilin reslkhvtfd vellgdcdvi ikelcnrlgw
      481 dvdsdeepva plteltslpp qvsaksvsei repaeqrgnv aadengntps sstaigeevm
      541 pdstcgemtp avesqsdsld csseclwkpr iresvasrlp eghylftsps tyifsgaevy
      601 vdsedsdspg asftgeeeed vesdsesshs tfssdeeesp ahtsglesps qgntmdcdpa
      661 eqgpstgpts mplvgnlgef ych
//
LOCUS       XP_077504573             684 aa            linear   INV 25-NOV-2025
DEFINITION  sirtuin 1 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504573
VERSION     XP_077504573.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648447.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..684
                     /product="sirtuin 1 isoform X1"
                     /calculated_mol_wt=74187
     Region          <199..241
                     /region_name="DUF592"
                     /note="Protein of unknown function (DUF592); pfam04574"
                     /db_xref="CDD:368003"
     Region          235..475
                     /region_name="SIRT1"
                     /note="Eukaryotic group (class1) which includes human
                     sirtuins SIRT1-3 and yeast Hst1-4; and are members of the
                     SIR2 family of proteins, silent information regulator 2
                     (Sir2) enzymes which catalyze NAD+-dependent
                     protein/histone deacetylation. Sir2 proteins...; cd01408"
                     /db_xref="CDD:238699"
     Site            order(244,246..247,254..255,308,326..327,329,344,426,431,
                     452..453,467)
                     /site_type="other"
                     /note="NAD+ binding site [chemical binding]"
                     /db_xref="CDD:238699"
     Site            order(328,344,398,400..404,430..432)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:238699"
     Site            order(352,355,376,379)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:238699"
     CDS             1..684
                     /gene="Sirt1"
                     /coded_by="XM_077648447.1:10..2064"
                     /db_xref="GeneID:144114597"
ORIGIN      
        1 madrreassa spaykrprle ndsnllglsi pndvvmppke fkrqrfasda anevtsssda
       61 nsemcngdsg fhelssttgg dmvtadgvcp dndhlvtagv arevdssghl flgalgavdv
      121 apkddicddd adevastisq lsglsdlsdl agaewkptse gpmgwvqrqm slgadprsll
      181 erllpdgavi pssldrltlw kvlismlsee epprrtklah vntlddvvsl lrtcqrvlvl
      241 tgagvsvscg ipdfrsrngi yarlskdfpa lpdpqamfdi hyfrkdprpf fkfakeiypg
      301 qftpsashrf ikllednnkl lrnytqnidt leqtcgirnv itchgsfata sctrchhkvd
      361 cnmikedifs qripvcpkcs peeveasgem avmkpdivff geglsqefhq amsrdksqcd
      421 llivmgsslk vrpvalipss ippevpqili nreslkhvtf dvellgdcdv iikelcnrlg
      481 wdvdsdeepv aplteltslp pqvsaksvse irepaeqrgn vaadengntp ssstaigeev
      541 mpdstcgemt pavesqsdsl dcsseclwkp riresvasrl peghylftsp styifsgaev
      601 yvdsedsdsp gasftgeeee dvesdsessh stfssdeees pahtsglesp sqgntmdcdp
      661 aeqgpstgpt smplvgnlge fych
//
LOCUS       XP_077504574             639 aa            linear   INV 25-NOV-2025
DEFINITION  sirtuin 1 isoform X3 [Amblyomma americanum].
ACCESSION   XP_077504574
VERSION     XP_077504574.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648448.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..639
                     /product="sirtuin 1 isoform X3"
                     /calculated_mol_wt=69614
     Region          <154..196
                     /region_name="DUF592"
                     /note="Protein of unknown function (DUF592); pfam04574"
                     /db_xref="CDD:368003"
     Region          190..430
                     /region_name="SIRT1"
                     /note="Eukaryotic group (class1) which includes human
                     sirtuins SIRT1-3 and yeast Hst1-4; and are members of the
                     SIR2 family of proteins, silent information regulator 2
                     (Sir2) enzymes which catalyze NAD+-dependent
                     protein/histone deacetylation. Sir2 proteins...; cd01408"
                     /db_xref="CDD:238699"
     Site            order(199,201..202,209..210,263,281..282,284,299,381,386,
                     407..408,422)
                     /site_type="other"
                     /note="NAD+ binding site [chemical binding]"
                     /db_xref="CDD:238699"
     Site            order(283,299,353,355..359,385..387)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:238699"
     Site            order(307,310,331,334)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:238699"
     CDS             1..639
                     /gene="Sirt1"
                     /coded_by="XM_077648448.1:140..2059"
                     /db_xref="GeneID:144114597"
ORIGIN      
        1 madrreassa spaykrprle ndsnllglsi pndvvmppke fkrqrfasda anagvarevd
       61 ssghlflgal gavdvapkdd icdddadeva stisqlsgls dlsdlagaew kptsegpmgw
      121 vqrqmslgad prsllerllp dgavipssld rltlwkvlis mlseeepprr tklahvntld
      181 dvvsllrtcq rvlvltgagv svscgipdfr srngiyarls kdfpalpdpq amfdihyfrk
      241 dprpffkfak eiypgqftps ashrfiklle dnnkllrnyt qnidtleqtc girnvitchg
      301 sfatasctrc hhkvdcnmik edifsqripv cpkcspeeve asgemavmkp divffgegls
      361 qefhqamsrd ksqcdllivm gsslkvrpva lipssippev pqilinresl khvtfdvell
      421 gdcdviikel cnrlgwdvds deepvaplte ltslppqvsa ksvseirepa eqrgnvaade
      481 ngntpsssta igeevmpdst cgemtpaves qsdsldcsse clwkprires vasrlpeghy
      541 lftspstyif sgaevyvdse dsdspgasft geeeedvesd sesshstfss deeespahts
      601 glespsqgnt mdcdpaeqgp stgptsmplv gnlgefych
//
LOCUS       XP_077504575             621 aa            linear   INV 25-NOV-2025
DEFINITION  sirtuin 1 isoform X4 [Amblyomma americanum].
ACCESSION   XP_077504575
VERSION     XP_077504575.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648449.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..621
                     /product="sirtuin 1 isoform X4"
                     /calculated_mol_wt=67279
     Region          <136..178
                     /region_name="DUF592"
                     /note="Protein of unknown function (DUF592); pfam04574"
                     /db_xref="CDD:368003"
     Region          172..412
                     /region_name="SIRT1"
                     /note="Eukaryotic group (class1) which includes human
                     sirtuins SIRT1-3 and yeast Hst1-4; and are members of the
                     SIR2 family of proteins, silent information regulator 2
                     (Sir2) enzymes which catalyze NAD+-dependent
                     protein/histone deacetylation. Sir2 proteins...; cd01408"
                     /db_xref="CDD:238699"
     Site            order(181,183..184,191..192,245,263..264,266,281,363,368,
                     389..390,404)
                     /site_type="other"
                     /note="NAD+ binding site [chemical binding]"
                     /db_xref="CDD:238699"
     Site            order(265,281,335,337..341,367..369)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:238699"
     Site            order(289,292,313,316)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:238699"
     CDS             1..621
                     /gene="Sirt1"
                     /coded_by="XM_077648449.1:150..2015"
                     /db_xref="GeneID:144114597"
ORIGIN      
        1 mcngdsgfhe lssttggdmv tadgvcpdnd hlvtagvare vdssghlflg algavdvapk
       61 ddicdddade vastisqlsg lsdlsdlaga ewkptsegpm gwvqrqmslg adprsllerl
      121 lpdgavipss ldrltlwkvl ismlseeepp rrtklahvnt lddvvsllrt cqrvlvltga
      181 gvsvscgipd frsrngiyar lskdfpalpd pqamfdihyf rkdprpffkf akeiypgqft
      241 psashrfikl lednnkllrn ytqnidtleq tcgirnvitc hgsfatasct rchhkvdcnm
      301 ikedifsqri pvcpkcspee veasgemavm kpdivffgeg lsqefhqams rdksqcdlli
      361 vmgsslkvrp valipssipp evpqilinre slkhvtfdve llgdcdviik elcnrlgwdv
      421 dsdeepvapl teltslppqv saksvseire paeqrgnvaa dengntpsss taigeevmpd
      481 stcgemtpav esqsdsldcs seclwkprir esvasrlpeg hylftspsty ifsgaevyvd
      541 sedsdspgas ftgeeeedve sdsesshstf ssdeeespah tsglespsqg ntmdcdpaeq
      601 gpstgptsmp lvgnlgefyc h
//
LOCUS       XP_077504576             356 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114598 [Amblyomma americanum].
ACCESSION   XP_077504576
VERSION     XP_077504576.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648450.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..356
                     /product="uncharacterized protein LOC144114598"
                     /calculated_mol_wt=39130
     Region          99..>125
                     /region_name="DUF3719"
                     /note="Protein of unknown function (DUF3719); pfam12516"
                     /db_xref="CDD:463617"
     CDS             1..356
                     /gene="LOC144114598"
                     /coded_by="XM_077648450.1:476..1546"
                     /db_xref="GeneID:144114598"
ORIGIN      
        1 mavcvrrskr ergqpltsgq nvvietdepp sddetvvycv dcssgtgfsp wsehtaptat
       61 sswdedfnas ataqvqqtld aidcylyede tggaacnhel rlecdrwkqq flhlrvigkr
      121 itspamgvvk nilpnngsas aeskcdteps wsaedealkn kvvdqlcdmf wpevqlwldl
      181 shqhldldhl slrqsdwevd revsncchgl egvltvtpkl lsdrflersg ssslfssrss
      241 taqsvlregs rqlwscdgqq wqqqspavsr cisgpqpwlp plssgsnsss sskvpmssqs
      301 srrsdrtssg ssasklppid paslqsiyve ghrinvserp lyqsgnsccc rprank
//
LOCUS       XP_077504577             356 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114598 [Amblyomma americanum].
ACCESSION   XP_077504577
VERSION     XP_077504577.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648451.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..356
                     /product="uncharacterized protein LOC144114598"
                     /calculated_mol_wt=39130
     Region          99..>125
                     /region_name="DUF3719"
                     /note="Protein of unknown function (DUF3719); pfam12516"
                     /db_xref="CDD:463617"
     CDS             1..356
                     /gene="LOC144114598"
                     /coded_by="XM_077648451.1:296..1366"
                     /db_xref="GeneID:144114598"
ORIGIN      
        1 mavcvrrskr ergqpltsgq nvvietdepp sddetvvycv dcssgtgfsp wsehtaptat
       61 sswdedfnas ataqvqqtld aidcylyede tggaacnhel rlecdrwkqq flhlrvigkr
      121 itspamgvvk nilpnngsas aeskcdteps wsaedealkn kvvdqlcdmf wpevqlwldl
      181 shqhldldhl slrqsdwevd revsncchgl egvltvtpkl lsdrflersg ssslfssrss
      241 taqsvlregs rqlwscdgqq wqqqspavsr cisgpqpwlp plssgsnsss sskvpmssqs
      301 srrsdrtssg ssasklppid paslqsiyve ghrinvserp lyqsgnsccc rprank
//
LOCUS       XP_077504578            2244 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC144114600
            [Amblyomma americanum].
ACCESSION   XP_077504578
VERSION     XP_077504578.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648452.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2244
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC144114600"
                     /calculated_mol_wt=227852
     Region          93..139
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          144..183
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          150..>212
                     /region_name="ANAPC4_WD40"
                     /note="Anaphase-promoting complex subunit 4 WD40 domain;
                     pfam12894"
                     /db_xref="CDD:403945"
     Region          192..226
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          431..>594
                     /region_name="Nucleoporin_FG2"
                     /note="Nucleoporin FG repeated region; pfam15967"
                     /db_xref="CDD:435043"
     Region          1544..>1753
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     CDS             1..2244
                     /gene="LOC144114600"
                     /coded_by="XM_077648452.1:202..6936"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 2 bases in 1 codon"
                     /db_xref="GeneID:144114600"
ORIGIN      
        1 macegstrpn pdflfhpvrt vnvtvggesv sadsvnlvaa ssrfglifag fdqgvkictl
       61 ediqncdaee gpnskapaen cpfrllpttg pvllvalnad dttlavglmr dgvpvadmyd
      121 vrgfggqtle trpiatvrls tlpdvalqdf swnpvacdmf avclnngsvs iyelsgealt
      181 ilgtlppsvg gtalcwspkg kqlvvgkkdg slsqykpnmq eakshssptl fensvsvvsv
      241 cwlaptafav vyasatsted sqanlvtitf sktspitytn fgepcyksgq hprdrfwlyh
      301 qpdwgilvcm srnsiesavl gcksadkmqw elweldshgr aevplrrgee ayplglavsy
      361 naqrritskm eswapmpill vlstcgglsp fhiknlsqgv pslvrvpepl lpagqrkpma
      421 nppqlaaqqt aqvaspttst gflpvghaaa tpvaplgaaa asrvtahrkp svgsatapql
      481 igsgtatvps ftsqpfpssg sfapvatsag apaptslsga gstslfsslp asstamafsf
      541 gssannafsf gttgggqafs fapassavtv skstpalals gtsssaffst papsfatatt
      601 tvpaapplsm afstptassa atpaapafgi pasaasgasg lqfklraktd aeapggapqv
      661 ptilptpsqp vvaqetsaqe antallgasa pkgstpaata pasppseepc kaepsedaii
      721 atmneeivel gremeafkkk vlcraiepag sdaglmkmkk lgvalestae tlrsdmekth
      781 vdvhtlrslv fetfalleea rtrdlrnrdp qyagllrdrg ldpvaarrla earrlrhyvq
      841 eqlrevharl dldwrehvdq lssrkrtael staeaiyral rdihntaell sktvgllgaq
      901 lnrlrvvskh cktlrpcwde ssilnreeev aaladslllt slsassarat tvhlakpnkv
      961 tlsaekknyl aeffgqrevt tieprvidkh sesrllsrla vvmeesfakd qaakqqasqa
     1021 vtqapmskpa qtqqqglvst pstpvkptal leardniptp kafvpaqgat qsfltstplv
     1081 rtegvassgt ssgvplirav pteqhrmpet kvievsvqas ggldykssav pptvfpaglt
     1141 vsslaaappe issaavtnvv tkplptrset tvttkeagsp spftspfvvp rggslpagtq
     1201 pglkfklpgq ttsdtlpfts afsfgpattt sassaasatv lsfakpsgta aptatsssfk
     1261 fgdstaskpg fmavkplfgp sftpvsqptg lqtgaptekk teatsveage eepayeditp
     1321 pgsptpdqeg eeplnaepal sltkdipvsl adtsvpavps aavfsftsat tggspslsss
     1381 tsapaplfqq gfgqpvttaa kslfgnssms ssasgaaaaf sftaakdspa mnifatpqvk
     1441 gpgsgegqpn ttqakslfgn ssvssasaaa aafsftaakd spamnifaip qvkgpgsget
     1501 rppsgtvlav kdnsskdavk aplmfgpsgp salkppasat gntavsgaea tvskspfqpv
     1561 ssaasqpeif tltttaasaq psdaqtlsak tptsepsktd amvaaattaa iaasiaasls
     1621 sptiatsvpa sapaslavst pssiastqaa viaaellsst ppsspqppst psevvaptsc
     1681 sgtvlglgss svfggaslpk tpttvlfgqq avstapvtpt sfsfgsvpts laglvsqapa
     1741 tliatqptgq tatsviatpv fgqaagttvt tpsstpaqqa fsipqmavas sagtlpfgsq
     1801 lttttssalt fgqvaattaa avstpsvfgq paaagasmsa mfgtttssat sptstpslfg
     1861 qnratftsst tttttapfwk pvasgatifg qpagqsifgq aptsaassfg qqmgttpafg
     1921 qpggftfgqs pvvsssagss pfgqiipata sggtssslfg qkssfgtssg qtpslfgqqs
     1981 lfgqsstpaf gqpqaasgfg qqsqdqqqqg ggsslfgtsg sgflsglgsk paadapnknv
     2041 fggsstfsss gqtsnlfgnq gassfgsstf gsavgagtfs gggsfsmtag svaqsgfgaf
     2101 qqqqqqqqtp tkaaafggpp sfggsptfgg ppqfggspaf ggspafggaa tfgsttspqq
     2161 spqqssqqqq qqqqqqqqqs gfmmfsnveg ptfgglaaqs stqqqqgmgf gnafgspgfg
     2221 gpspgfgsqn aqpnsapafs qwrn
//
LOCUS       XP_077504580             286 aa            linear   INV 25-NOV-2025
DEFINITION  eukaryotic translation initiation factor 3 subunit G-like
            [Amblyomma americanum].
ACCESSION   XP_077504580
VERSION     XP_077504580.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648454.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..286
                     /product="eukaryotic translation initiation factor 3
                     subunit G-like"
                     /calculated_mol_wt=31995
     Region          28..149
                     /region_name="eIF3g"
                     /note="Eukaryotic translation initiation factor 3 subunit
                     G; pfam12353"
                     /db_xref="CDD:463545"
     Region          205..280
                     /region_name="RRM_eIF3G_like"
                     /note="RNA recognition motif (RRM) found in eukaryotic
                     translation initiation factor 3 subunit G (eIF-3G) and
                     similar proteins; cd12408"
                     /db_xref="CDD:409842"
     CDS             1..286
                     /gene="LOC144114601"
                     /coded_by="XM_077648454.1:134..994"
                     /db_xref="GeneID:144114601"
ORIGIN      
        1 mpvadsanqp swadrveqge dvplpslppv qveladgeek vttefredee grkvkvlrhy
       61 kverrqvsks varrklwkkf gaakndppgp naattvvgee iqmqfvsaqe eetspeedml
      121 aklrsqktkm vkcrickedh wttqcpfkdk valpehlkee akpaaaveve ekvksgkyip
      181 psmreganrr gesmtqsrtr deaatirvtn lsedvrdsdl qdlfrpfgqi ariylakdkv
      241 tgqskgfafi nfvhredaar aianvngfgy dnlilsvewa kpsgta
//
LOCUS       XP_077504581             355 aa            linear   INV 25-NOV-2025
DEFINITION  GPI-anchor transamidase-like [Amblyomma americanum].
ACCESSION   XP_077504581
VERSION     XP_077504581.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648455.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..355
                     /product="GPI-anchor transamidase-like"
                     /calculated_mol_wt=40646
     Region          46..307
                     /region_name="CASc"
                     /note="Caspase, interleukin-1 beta converting enzyme (ICE)
                     homologues; Cysteine-dependent aspartate-directed
                     proteases that mediate programmed cell death (apoptosis).
                     Caspases are synthesized as inactive zymogens and
                     activated by proteolysis of the peptide...; cl00042"
                     /db_xref="CDD:444667"
     CDS             1..355
                     /gene="LOC144114602"
                     /coded_by="XM_077648455.1:212..1279"
                     /db_xref="GeneID:144114602"
ORIGIN      
        1 mipwklgvyf tvslmsllvl psqsqhases vdakvseffr sghtnnwavl vctsrfwfny
       61 rhvanvlsiy rsvkrfgipd sqiilmiadd mpcnlrnpkp atvfnnahhh invygdnvev
      121 dyrgyevtve nfiriltgrl pantprskrl ltdeysnili ymtghggdgf lkfqdseevt
      181 nveladafeq mwqkrryhei ffmidtcqae smfkrfyspn ilavasskig edslshhgdp
      241 tigvyvidry tyyalmflee vttnntktma qflrvcpksv cistavsrkd lftrnldqvp
      301 ltdffgsvrn veltrhkvtl rpllapsqav gkqpkntilt qkqewhkigq fsfls
//
LOCUS       XP_077504582            1064 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114603 [Amblyomma americanum].
ACCESSION   XP_077504582
VERSION     XP_077504582.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648456.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1064
                     /product="uncharacterized protein LOC144114603"
                     /calculated_mol_wt=118449
     Region          274..293
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(279,281,283,285..286,289..290,293,306,308,312..313,
                     316..317,320,336,338,340,342..343,346..347)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          301..321
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          331..347
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          484..504
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(484,487,500,504)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          522..542
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          553..573
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(553,556,569,573)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          584..605
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(584,587,600,605)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(589,591,593,595..596,599..600,604,618,620,636..637,
                     669..670,673,687,689,691,693..694,697..698,702)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          613..674
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          682..703
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          710..730
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(710,713,726,730)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(715,717,719,721..722,725..726,729,743,745,749..750,
                     753..754,757,771,773,775,777..778,781..782,785)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          738..811
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          738..758
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          738..758
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(743,749,757,765,772)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          766..787
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          766..786
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          807..827
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          838..858
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(838,841,854,858)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(843,845,847,849..850,853..854,857,870,872,876..877,
                     880..881,884,899,901,903,905..906,909..910,913)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          865..886
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          894..913
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1064
                     /gene="LOC144114603"
                     /coded_by="XM_077648456.1:364..3558"
                     /db_xref="GeneID:144114603"
ORIGIN      
        1 masnehrtfi yldfdidpft aelvfpqgip kggslslvfg afskgqptqk akfteefarm
       61 vsqkfrvssp lvdgspqcfi rislakdgae ppqiprpnkt vsllkklphl plpppaslns
      121 qfpmaidldd eeenedsdlr itttgrgvqp asgaknssaq lraaivpaaa spmaaasaiv
      181 kpavvrlaat aaktaavkpa aakpaaakpa aakpaaakpa aaksvvmtpa aangsvedds
      241 dasiesrpgs pfdddtgage gagsdgdevg tfccchcams fhfeselqkh qavvhdmlea
      301 ckhcskkfps kfslvrhmws hlprsrlpfr cseckkgfit krellghkak phvrkvrvpq
      361 qltggagmqt ehtldfqeal eepscserhs ysapteeeif fcefcsevyl tghfyrqhvs
      421 khhaekldmy pvdissftgv ekdpdreade kfrcslckdc aglsfrevcc hvsdahpsag
      481 vhackrctlv fsneeeyeah ssshivkvtp sltspgfkdy hcnlcsevlt hrktlvrhlq
      541 qhhrdpnvkp yacglcpsrf rgkaslyiht rkhntqverr rfpctqcpki ftakqflkrh
      601 eeavharllp facthcskrf yferhlqqhl vmshrsklse eevaglglih nfqceecgfs
      661 sysertirrh vyshtgtypf tcevcnrgfv frfeltahha rrhlhqklhc hlcsriffik
      721 erfdkhlsah qekwgftcsv cgqlfetqgy lenhqlrhsg ktpyectecg krfsapqglt
      781 fhkqqhhhak rprqvdnarm ehwplgcdic girfkylssq qahmafhhps segeslqcsy
      841 cqrklgskla lsqhlrkhtn ekykckhcdr cfqsyvgcrt hqvvmhtrrf ktacpfckkg
      901 cvsifdlkkh lrtvhnavvv kqqnlemvgs gdsaaiemld etmvvgedai evvgedgvqv
      961 igkegmqivg egaiqvvged gvevigkegm hivgegaiqv vgddgiqivs seeameiisq
     1021 qgmniakeqy ihpvgqdvqv vseqdvetrs evvlvelgea pvlm
//
LOCUS       XP_077504583             408 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114605 [Amblyomma americanum].
ACCESSION   XP_077504583
VERSION     XP_077504583.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648457.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..408
                     /product="uncharacterized protein LOC144114605"
                     /calculated_mol_wt=45506
     CDS             1..408
                     /gene="LOC144114605"
                     /coded_by="XM_077648457.1:227..1453"
                     /db_xref="GeneID:144114605"
ORIGIN      
        1 madgqrrfst qrgtyqanfl lyprgappqh aqaavpmhks esalslsmgr psspipplhq
       61 rrgtictsev mrvesnttva pcsdqamppm aeammttppl qqqqqpqqqs qqqqppatir
      121 rsymvtsfae ppttmlsgyg pcsgydrpys pappprppsi hrsssarrsp vrrprsaygy
      181 ddyyddrrrr ncdddfgddd yecdersfrr apqrsrsgrd vdmgaksptp rsgsrlrvmr
      241 iereisdtgs arqrtpsasr glsrgrlgee tegadscvci cgsarsppsr grlsgiyikp
      301 rreedeelyv ikrrrsrrkn krkrytpppp ppsspsqpgl vdylcslvgm ktseeapptp
      361 papppsaatd sdteddadye lkrldraele rlcrqidfar sanasdkk
//
LOCUS       XP_077504584             791 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114606 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504584
VERSION     XP_077504584.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648458.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..791
                     /product="uncharacterized protein LOC144114606 isoform X1"
                     /calculated_mol_wt=85567
     Region          57..>418
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     Region          604..787
                     /region_name="aden_kin_iso1"
                     /note="adenylate kinase, isozyme 1 subfamily; TIGR01360"
                     /db_xref="CDD:130427"
     CDS             1..791
                     /gene="LOC144114606"
                     /coded_by="XM_077648458.1:152..2527"
                     /db_xref="GeneID:144114606"
ORIGIN      
        1 maseqsqraq tlryqsnttf ligpnaaglg ggglpgqlqr nsqtaitttp gggrawppqa
       61 lgseapvaav easellqvgs ntvvspppqp ttgyaspvvr rsqmttslte msavpgqsaa
      121 sappnsigsa gsvsavppsp lmsrppshgp mrrpnlptsp ndghrfgrpa aqpppagsag
      181 svtppsprhp yfassvtesp gsvaissvig cpspsnnslg stppgvrspg fppqrmmast
      241 gqprplppgr qfhgpifpdn eirrqpissv dfreedride gyealsgert ppvpparqpa
      301 tagsrsgrrl pvgpsrlppa pgsrvgsgps vsrlrmfqie reldapsppp vrerlqqdrq
      361 ldenredgpp pasplmrrrs fedttlhddl agprpkvcnc pgtvlvrrgp prrstsgrlr
      421 gayvtdaded mddlfmikka kkkkkkkqpt pppppppvap ppggfmgfls glvgghqppp
      481 ppppspqqqa atlasdsesd deseyelrrf drrqlenfvr kidlkqlmqe seenkegrka
      541 kvkeekkeek keekkeekke ekkveekkpe ekkpeekaee apppakpapp ptstpmdgpl
      601 elnvpvvfvf ggpgsgkgtq cekivakygf thissgdllr aevqsgsdlg kemneimtkg
      661 elvplvmvlq llkeairksv atakgflidg yprnteqgdr feaevckctn llyfevkdet
      721 mkerllkrge tsgrvddnee titkriktfh eesepvleky ktivhkvsae edpdkvfeav
      781 taffdeitkp k
//
LOCUS       XP_077504585             688 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114606 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504585
VERSION     XP_077504585.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648459.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..688
                     /product="uncharacterized protein LOC144114606 isoform X2"
                     /calculated_mol_wt=75086
     Region          4..>315
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     Region          501..684
                     /region_name="aden_kin_iso1"
                     /note="adenylate kinase, isozyme 1 subfamily; TIGR01360"
                     /db_xref="CDD:130427"
     CDS             1..688
                     /gene="LOC144114606"
                     /coded_by="XM_077648459.1:297..2363"
                     /db_xref="GeneID:144114606"
ORIGIN      
        1 mttsltemsa vpgqsaasap pnsigsagsv savppsplms rppshgpmrr pnlptspndg
       61 hrfgrpaaqp ppagsagsvt ppsprhpyfa ssvtespgsv aissvigcps psnnslgstp
      121 pgvrspgfpp qrmmastgqp rplppgrqfh gpifpdneir rqpissvdfr eedridegye
      181 alsgertppv pparqpatag srsgrrlpvg psrlppapgs rvgsgpsvsr lrmfqierel
      241 dapspppvre rlqqdrqlde nredgpppas plmrrrsfed ttlhddlagp rpkvcncpgt
      301 vlvrrgpprr stsgrlrgay vtdadedmdd lfmikkakkk kkkkqptppp ppppvapppg
      361 gfmgflsglv gghqpppppp pspqqqaatl asdsesddes eyelrrfdrr qlenfvrkid
      421 lkqlmqesee nkegrkakvk eekkeekkee kkeekkeekk veekkpeekk peekaeeapp
      481 pakpapppts tpmdgpleln vpvvfvfggp gsgkgtqcek ivakygfthi ssgdllraev
      541 qsgsdlgkem neimtkgelv plvmvlqllk eairksvata kgflidgypr nteqgdrfea
      601 evckctnlly fevkdetmke rllkrgetsg rvddneetit kriktfhees epvlekykti
      661 vhkvsaeedp dkvfeavtaf fdeitkpk
//
LOCUS       XP_077504586             121 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114607 [Amblyomma americanum].
ACCESSION   XP_077504586
VERSION     XP_077504586.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648460.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..121
                     /product="uncharacterized protein LOC144114607"
                     /calculated_mol_wt=13350
     CDS             1..121
                     /gene="LOC144114607"
                     /coded_by="XM_077648460.1:1018..1383"
                     /db_xref="GeneID:144114607"
ORIGIN      
        1 mlarselaal hynenagrvq crttigrrqw avkhpkawkg npvacpvkep atfgyvehlp
       61 eivdelvesg sfgathvttl pqppppltss fgpvdkaalv rdhlsrfesr eeqgrhterc
      121 q
//
LOCUS       XP_077504587             549 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114609 [Amblyomma americanum].
ACCESSION   XP_077504587
VERSION     XP_077504587.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648461.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..549
                     /product="uncharacterized protein LOC144114609"
                     /calculated_mol_wt=61095
     Region          17..103
                     /region_name="LOTUS_TDRD_OSKAR"
                     /note="The first LOTUS domain in Oskar and
                     Tudor-containing proteins 5 and 7; cd09972"
                     /db_xref="CDD:193586"
     Region          126..>173
                     /region_name="LabA_like_C"
                     /note="C-terminal domain of LabA_like proteins; cl14879"
                     /db_xref="CDD:472713"
     Region          266..379
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     CDS             1..549
                     /gene="LOC144114609"
                     /coded_by="XM_077648461.1:244..1893"
                     /db_xref="GeneID:144114609"
ORIGIN      
        1 mgmaattkpm gdrardlndv kcvirallvv nngpttcaal mkdyrtqegc qlpyvrfgyt
       61 dcvtfleslp dtvhvqrvgd dvlisaklde rirhiqtlvq aqkardhapr pvvrrkrerp
      121 sppqlpeavl enilkllreg dvharvlrda yyrrfgvpld ieqygfasle dclarvrlva
      181 enekglpeav vavlaqypeg iymsrfseaf arergrppts rtialvqrwp qlfrvqkpds
      241 sgdfilqpai ssaaillppe plpvrssvrv tdvccspaal tvallapvne splallgaam
      301 dhfygptafg ppfdakpgrp caalcaetwr rarvvtvtgn ivqvelvdvg grqslaphrl
      361 rplaprftel palctmatlt lppgtrawck tagerlrelt kscqltclfd gtrfelqdeq
      421 gllltrqlas eglckpawrr vtlsdevsfn vlwldgqryi fsydlsrlvg ragdsaleel
      481 qrrgirfqcv hlyrnselwy algpllgtsi havrlfpadn iadavnilcy phtqvaievr
      541 relakfqds
//
LOCUS       XP_077504588             370 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114610 [Amblyomma americanum].
ACCESSION   XP_077504588
VERSION     XP_077504588.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648462.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 35% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..370
                     /product="uncharacterized protein LOC144114610"
                     /calculated_mol_wt=40620
     CDS             1..370
                     /gene="LOC144114610"
                     /coded_by="XM_077648462.1:87..1199"
                     /db_xref="GeneID:144114610"
ORIGIN      
        1 maspgasslv kfnirwgnrs nqssimntse yidtlkkrlr avimknkila ralaaskkei
       61 selkksngrq qkvfnlglpq vktwlkmarq hshtmyqyqs raltlldeim npgvdftlar
      121 vsdadddadi qdrrpslhpp gkrskpqrge lsvipeeded vnasrrmllg tftldsetrl
      181 raaetierks rgsmssserp ssssgkprap tpkgsvqslg ckvrgpwwtd ggagdldyaf
      241 lespnptapl rqgnepgekm vtvdaqsrip evadpqhqet asekpsssts tadldnapre
      301 sflpnkrkee adgqefvglk padtlvanga eaygvdqrsp rqinrhppra rchmsspspg
      361 esedttafgk
//
LOCUS       XP_077504590            1469 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC144114611
            [Amblyomma americanum].
ACCESSION   XP_077504590
VERSION     XP_077504590.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648464.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 6 indels
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1469
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC144114611"
                     /calculated_mol_wt=156724
     CDS             1..1469
                     /gene="LOC144114611"
                     /coded_by="XM_077648464.1:133..4542"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 2 bases in 2 codons;
                     deleted 5 bases in 4 codons"
                     /db_xref="GeneID:144114611"
ORIGIN      
        1 mtqqdaprwe phvlcvagla apapeedysg sstggscssd atrlesassh geedsdsgsw
       61 wsypsecgsv velnravaaa tngfagggss nnrhhdsten gnaltkpsey atappvsspd
      121 rhdiviatav vsndagrkap ngvqrggavi ipsspdrpps ksrcvngrsr sasddnpqdy
      181 darnrgnasy aatixnrsld pstrrasiag veglttadhh nsqvrtgsra lpkpagkdvn
      241 sgrsvairks alapetrhsa psivrhvtfk dlssassgsd dddytsgqgr nrnsasyhgd
      301 dqqnnaeadl ddsgfldgds tssssdispp sslswesaid ssssrkshgr sadqqcfvds
      361 gggdsrngth sppxprcesr agvaeerrtk smngthregd gnstlsfvqt eicvsqsvrr
      421 edsyeyyrng triaklnnvn qtswsetsst etterctplg kssgiektvi reqttpqakp
      481 hssqvddaak tsappqparr vlpsvdaark rqpeaaaqgr qsatresgkq kkesaggggv
      541 rnvggsvnpt vvssaandac qqrdyhrcrq qkaaqppita yddegtscdl edeedgaaer
      601 qllededvsf sfesppaahh qkqragtvsa tatsravars rggsstaeps rasalasvra
      661 qsrdpflala vatakaatta saeasreiya gckqfapppd ldvvvrtacs vggsedavdr
      721 arharsqpsd divsvssspv yrvggtstrs dvrlptpvry kefsslhlkk sgggrehang
      781 hygegdvspl lereplpceq rsfhvhsdvl psradssppt sstpspvlle drvrvlqtpv
      841 ksnaiivdni pdsddcdgpp itstpkvsng hcsnkrpatl pvngaagsvc hrkpppfpqq
      901 qkqlasqpll lrppkpvppp rsrspldpae hleritgyat arrlaqtlpk pqvqlpephv
      961 spasgrlvss rrrffenlqr qqeqtkrtse ataeesaerl lgfrasaqaa reslaksspd
     1021 laaleadawr srrlhdrwpp pstesphapr qpaepignaa vlpprspapa vhpdkfnrlv
     1081 eqtrsryarr rppahderrr srslgyletd vdtlccrqvk gpaaeeegaq daaddaylnr
     1141 arsmdrfled gtvgcpllgd dqrtksehel rierslqkle lpewytrrak qtpaetgilr
     1201 arrdsassat ragwqglgsr tpstaslaat tpesrnlvip krvtapdwrc whasreslts
     1261 spgshdgsls rwgsvssapa aawsyrsmrq pylgwraaps tgtttplssa gsrpvspggg
     1321 wtaafdytqq qdqqehrqqq qqqspciprd rysyvqgiyg dgggwnvdre ddsaavcssv
     1381 isdvgvpsts apsngaarpa vwmessfvgs kpgstssvva aqspaapalv hitivykavr
     1441 qpsaapcpea tkplhtpate pntavhath
//
LOCUS       XP_077504591             361 aa            linear   INV 25-NOV-2025
DEFINITION  beta-1,3-galactosyltransferase 5-like [Amblyomma americanum].
ACCESSION   XP_077504591
VERSION     XP_077504591.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648465.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..361
                     /product="beta-1,3-galactosyltransferase 5-like"
                     /calculated_mol_wt=39392
     Region          90..307
                     /region_name="Galactosyl_T"
                     /note="Galactosyltransferase; cl21608"
                     /db_xref="CDD:473923"
     CDS             1..361
                     /gene="LOC144114612"
                     /coded_by="XM_077648465.1:174..1259"
                     /db_xref="GeneID:144114612"
ORIGIN      
        1 mtkllmvllr raavavclsf tslfvyyvlv rrghtdatkw sqpppaaata taillrrvvs
       61 sgaflsvprt pcpeflavvv csapehgper lairetware vrpehsavyf lvgtrpedng
      121 ttkpswtall kpgspetrgd ssllpessvf gdmirvdfvd tsgnstlktl lmlqwaqsyc
      181 prvrfvlkad ddafvdvprl isvlerkqga fenntqlsgs spgfllgkrq eaksvvmine
      241 srhdvvpgah htgglpayls gvayvlsggv vgplfrkale mpalhledvf ltglvaselg
      301 lslihspcfa cceelpdpca yrrllaatvs kpdslrnvwn rvrdkaaerc pprndtddhq
      361 c
//
LOCUS       XP_077504592             307 aa            linear   INV 25-NOV-2025
DEFINITION  beta-1,3-galactosyltransferase 1-like [Amblyomma americanum].
ACCESSION   XP_077504592
VERSION     XP_077504592.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648466.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..307
                     /product="beta-1,3-galactosyltransferase 1-like"
                     /calculated_mol_wt=34771
     Region          44..237
                     /region_name="Galactosyl_T"
                     /note="Galactosyltransferase; cl21608"
                     /db_xref="CDD:473923"
     CDS             1..307
                     /gene="LOC144114613"
                     /coded_by="XM_077648466.1:250..1173"
                     /db_xref="GeneID:144114613"
ORIGIN      
        1 mrwhvrqrdi iphvvgarpf lmlppaacpr ylaivvcsal shfearatvr dtwgrdaaak
       61 nvsvlfivgk pedkpsgrai geslknesar yrdviqadfr dsyrnltlkt vlllkwayvh
      121 csrtrfifka dddvfvnven llnflktyge sperpflagy vmqgkhavrs agskwylphs
      181 vyapdwlpmy ilgpgyvmsh gavrplfrea lltpflfied vfltgivahk vgieltnsqc
      241 fvphallwsc eyrsvisahl vsvaqlrnvw rhthnasiqc pplvkyngcr evrdekrgsl
      301 llrallt
//
LOCUS       XP_077504593             161 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114614 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504593
VERSION     XP_077504593.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648467.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..161
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..161
                     /product="uncharacterized protein LOC144114614 isoform X1"
                     /calculated_mol_wt=18836
     CDS             1..161
                     /gene="LOC144114614"
                     /coded_by="XM_077648467.1:114..599"
                     /db_xref="GeneID:144114614"
ORIGIN      
        1 mekreqgssp tkprnpfkrk iwfeasvdkn qqvsvemkkk crdlllraig nvlksypaas
       61 aetkvllhdt fenimkglnk afqlrvldps klekceqhye sllqqtkrkr vemeqcndrg
      121 rnccgwallr arwrlercce vvccyrchaf aseeskqykh p
//
LOCUS       XP_077504594             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114614 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504594
VERSION     XP_077504594.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648468.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144114614 isoform X2"
                     /calculated_mol_wt=18222
     CDS             1..158
                     /gene="LOC144114614"
                     /coded_by="XM_077648468.1:114..590"
                     /db_xref="GeneID:144114614"
ORIGIN      
        1 mekreqgssp tkprnpfkrk iwfeasvdkn qqvsvemkkk crdlllraig nvlksypaas
       61 aetkvllhdt fenimkglnk afqlrvldps klekceqhye sllqqtkrkr vemeqcndrg
      121 rklyevlrhq ygpengqess sssstvfvea sddtndew
//
LOCUS       XP_077504595             315 aa            linear   INV 25-NOV-2025
DEFINITION  stomatin-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504595
VERSION     XP_077504595.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648469.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..315
                     /product="stomatin-like isoform X1"
                     /calculated_mol_wt=34205
     Region          98..299
                     /region_name="SPFH_stomatin"
                     /note="Stomatin, a subgroup of the stomatin-like proteins
                     (slipins) family; belonging to the SPFH (stomatin,
                     prohibitin, flotillin, and HflK/C) superfamily; cd03403"
                     /db_xref="CDD:259801"
     CDS             1..315
                     /gene="LOC144114616"
                     /coded_by="XM_077648469.1:203..1150"
                     /db_xref="GeneID:144114616"
ORIGIN      
        1 msqegtgvgv rgakassgeg ashllsdlea gdmdkdhntg yrteahvetg sngvctfllt
       61 aisiffiivt fplsllmcik ivqeyeravi frlgrlvkgg argpglffii pcidnytkvd
      121 lrtvsfdvpp qeiltkdsvt vavdavvyyr iqnatvavtn vedygrstrl laattlrnvl
      181 gtkslseils ereaishtmq tnldeatdaw gvkvervemk dvrlpvqmqr amaaeaeasr
      241 earakviaae geqraarslk eaadvisesg palqlrylqt ltsiaaekns tivfplpmel
      301 fkgvlghpeh prarl
//
LOCUS       XP_077504596             315 aa            linear   INV 25-NOV-2025
DEFINITION  stomatin-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504596
VERSION     XP_077504596.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648470.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..315
                     /product="stomatin-like isoform X2"
                     /calculated_mol_wt=34137
     Region          98..299
                     /region_name="SPFH_stomatin"
                     /note="Stomatin, a subgroup of the stomatin-like proteins
                     (slipins) family; belonging to the SPFH (stomatin,
                     prohibitin, flotillin, and HflK/C) superfamily; cd03403"
                     /db_xref="CDD:259801"
     CDS             1..315
                     /gene="LOC144114616"
                     /coded_by="XM_077648470.1:203..1150"
                     /db_xref="GeneID:144114616"
ORIGIN      
        1 msqegtgvgv rgakassgeg ashllsdlea gdmdkdhntg yrteahvetg sngvctfllt
       61 aisiffiivt fplsllmcik ivqeyeravi frlgrlvkgg argpglffii pcidnytkvd
      121 lrtvsfdvpp qeiltkdsvt vavdavvyyr iqnatvavtn vedygrstrl laattlrnvl
      181 gtkslseils ereaishtmq tnldeatdaw gvkvervemk dvrlpvqmqr amaaeaeasr
      241 earakviaae geqraarslk eaadvisesg palqlrylqt ltsiaaekns tivfplpmel
      301 fkgvlgldrt psqdl
//
LOCUS       XP_077504597             309 aa            linear   INV 25-NOV-2025
DEFINITION  stomatin-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077504597
VERSION     XP_077504597.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648471.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..309
                     /product="stomatin-like isoform X3"
                     /calculated_mol_wt=33499
     Region          98..299
                     /region_name="SPFH_stomatin"
                     /note="Stomatin, a subgroup of the stomatin-like proteins
                     (slipins) family; belonging to the SPFH (stomatin,
                     prohibitin, flotillin, and HflK/C) superfamily; cd03403"
                     /db_xref="CDD:259801"
     CDS             1..309
                     /gene="LOC144114616"
                     /coded_by="XM_077648471.1:366..1295"
                     /db_xref="GeneID:144114616"
ORIGIN      
        1 msqegtgvgv rgakassgeg ashllsdlea gdmdkdhntg yrteahvetg sngvctfllt
       61 aisiffiivt fplsllmcik ivqeyeravi frlgrlvkgg argpglffii pcidnytkvd
      121 lrtvsfdvpp qeiltkdsvt vavdavvyyr iqnatvavtn vedygrstrl laattlrnvl
      181 gtkslseils ereaishtmq tnldeatdaw gvkvervemk dvrlpvqmqr amaaeaeasr
      241 earakviaae geqraarslk eaadvisesg palqlrylqt ltsiaaekns tivfplpmel
      301 fkgvldrss
//
LOCUS       XP_077504598             239 aa            linear   INV 25-NOV-2025
DEFINITION  stomatin-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077504598
VERSION     XP_077504598.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648472.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..239
                     /product="stomatin-like isoform X4"
                     /calculated_mol_wt=26293
     Region          22..223
                     /region_name="SPFH_like"
                     /note="core domain of the SPFH (stomatin, prohibitin,
                     flotillin, and HflK/C) superfamily; cl19107"
                     /db_xref="CDD:473137"
     CDS             1..239
                     /gene="LOC144114616"
                     /coded_by="XM_077648472.1:205..924"
                     /db_xref="GeneID:144114616"
ORIGIN      
        1 mcikivqeye ravifrlgrl vkggargpgl ffiipcidny tkvdlrtvsf dvppqeiltk
       61 dsvtvavdav vyyriqnatv avtnvedygr strllaattl rnvlgtksls eilsereais
      121 htmqtnldea tdawgvkver vemkdvrlpv qmqramaaea easrearakv iaaegeqraa
      181 rslkeaadvi sesgpalqlr ylqtltsiaa eknstivfpl pmelfkgvlg hpehprarl
//
LOCUS       XP_077504599             233 aa            linear   INV 25-NOV-2025
DEFINITION  stomatin-like isoform X5 [Amblyomma americanum].
ACCESSION   XP_077504599
VERSION     XP_077504599.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648473.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..233
                     /product="stomatin-like isoform X5"
                     /calculated_mol_wt=25588
     Region          22..223
                     /region_name="SPFH_like"
                     /note="core domain of the SPFH (stomatin, prohibitin,
                     flotillin, and HflK/C) superfamily; cl19107"
                     /db_xref="CDD:473137"
     CDS             1..233
                     /gene="LOC144114616"
                     /coded_by="XM_077648473.1:303..1004"
                     /db_xref="GeneID:144114616"
ORIGIN      
        1 mcikivqeye ravifrlgrl vkggargpgl ffiipcidny tkvdlrtvsf dvppqeiltk
       61 dsvtvavdav vyyriqnatv avtnvedygr strllaattl rnvlgtksls eilsereais
      121 htmqtnldea tdawgvkver vemkdvrlpv qmqramaaea easrearakv iaaegeqraa
      181 rslkeaadvi sesgpalqlr ylqtltsiaa eknstivfpl pmelfkgvld rss
//
LOCUS       XP_077504600             401 aa            linear   INV 25-NOV-2025
DEFINITION  RYamide receptor-like [Amblyomma americanum].
ACCESSION   XP_077504600
VERSION     XP_077504600.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648474.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..401
                     /product="RYamide receptor-like"
                     /calculated_mol_wt=45119
     Region          60..348
                     /region_name="7tmA_leucokinin-like"
                     /note="leucokinin-like peptide receptor from tick and
                     related proteins, member of the class A family of
                     seven-transmembrane G protein-coupled receptors; cd15393"
                     /db_xref="CDD:320515"
     Region          61..87
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320515"
     Region          94..119
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320515"
     Site            order(116,119..120,132..137,139..140,143,186,188..192,224,
                     227..229,231..233,235..236,293,296..297,299..300,303,
                     315..316,318..320,323,326..327)
                     /site_type="other"
                     /note="putative peptide ligand binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:320515"
     Region          132..162
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320515"
     Region          173..194
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320515"
     Region          224..253
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320515"
     Region          273..303
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320515"
     Region          316..341
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320515"
     CDS             1..401
                     /gene="LOC144114618"
                     /coded_by="XM_077648474.1:69..1274"
                     /db_xref="GeneID:144114618"
ORIGIN      
        1 mtsqpgmthd psapppllld spyvsqdygn lsllpsllpp hggnvnssgk lyqvpvgfiv
       61 llsifyglis lvavagnfmv mwivatsrrm qtvtnffian lavadiiigl fsipfqfqaa
      121 llqrwvlpef mcafcpfvqv lsvnvsiftl taialdryra vmsplkartt klrakfiicg
      181 iwtlavaaal pcalalrvet qveshalnlt kpfchevgis rsawriynha lvclqyffpl
      241 lticfvyarm glklkesksp gnaqgardag ilknkkkvik mlfvivalfa fcwlpyqlyn
      301 ilrevfpkid kykyiniiwf cthwlamsns cynpfiyaiy nerfkrefat rcicgsnryk
      361 aaksrfasye qednnstiiv smrhsfrlsf kgsplkastq v
//
LOCUS       XP_077504603             395 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114619 [Amblyomma americanum].
ACCESSION   XP_077504603
VERSION     XP_077504603.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648477.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..395
                     /product="uncharacterized protein LOC144114619"
                     /calculated_mol_wt=44269
     Region          285..375
                     /region_name="Spaetzle"
                     /note="pfam16077"
                     /db_xref="CDD:465007"
     CDS             1..395
                     /gene="LOC144114619"
                     /coded_by="XM_077648477.1:396..1583"
                     /db_xref="GeneID:144114619"
ORIGIN      
        1 mevygtmafl lcathsflsh pqvrsstspn htgtpvnsln vvstapspsk sfqakakpky
       61 rtedgerdqt pnastslmit lgervnwrrr grlinlfgsk krdqkpsvtq ahpalsiwek
      121 lrlpaeeply nasqtpfgwp nlwrwqaqdm vdqavdderh rrlfqvhhwm tavpkgvalh
      181 qspsdaetsg akqgiqqqts etsqhdvthh sssglvedfg pavgtpaapp csefnavycp
      241 ytedypidvv iqvtkflrwp leklfrdlrh lqsppladdg saalacdtvt rivrpgwakn
      301 tdgrwfvvin tlyyeqfvte iscryenapc nlvapcfyat cqqrynlqvl lvidptdpyk
      361 gpflsrflvp sccvcsapgg daqrtaedht yrdef
//
LOCUS       XP_077504604            1060 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114620 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504604
VERSION     XP_077504604.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648478.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1060
                     /product="uncharacterized protein LOC144114620 isoform X1"
                     /calculated_mol_wt=118429
     Region          669..757
                     /region_name="Spaetzle"
                     /note="pfam16077"
                     /db_xref="CDD:465007"
     CDS             1..1060
                     /gene="LOC144114620"
                     /coded_by="XM_077648478.1:672..3854"
                     /db_xref="GeneID:144114620"
ORIGIN      
        1 mlrrkrfyer ypaavvvvfl slnsisgtaa qvdrsnrepl dnanripwpv yqhstassip
       61 glvpnrppep pkqrlrlsds plppsfikvi nsvsspdwet ttpagpravv lttstttttt
      121 ttttpappps ttaplptssa yykrgqppev dplspsawka vpeippfpkr arlqakrrrk
      181 mrpkqetttk ertlftteva tevpttaypt rkfgvtrrql pslvrgyrsk ptkttppttt
      241 parrafvrpa lpgrkyspvi prrpvltttt ttstsstsss ttttttstrr pfvfgerkvp
      301 swygqtsttt tstttnrpll stpfsrlgrr pnlryksddi spgfgprprl plrtlppvir
      361 davpqtvekd elelsqsvqt qngrrrlvnd vparvhvvkg qlpdipavqi anngteavky
      421 egrhgasdeg gevvrfvaip hfvegsvpar radfkegarv pfrrifrnpf vvpvrrrfpy
      481 agpvavypps lssiedgssq deeviripvq rpesgafaal ppppglplrv vrpaypgavg
      541 pqagaalppa pptnnavqep ayapprppms lgsailpsrv srpkpeeprc drfsedicld
      601 dfeyptaail dsisrqrarf dtmyaevrdr saqvdgvtrk qeerytprhy fgaserdsrp
      661 rdyapdggfl cpseilyerp rrarnhlgvw kvivnigdyr qtmrlekclr pnrpcsyvle
      721 kyssscsqvy syqrlltfek gkglhidifr vpsgcschvk gfspfqrhde pitpvrqqqp
      781 yeydrttyts atslaaakep ssggrqvnnt lwiilgngke ldpetkerlv qqlrqypqla
      841 eqlpqgeavr elvneadgyq rpyarpeqtr ppsygvhysg hktreerphl vnsgavpprw
      901 aqqgpavaae plkyvqpnri rrppppppsd yeteealppg arpryyqkpf kapeedalft
      961 sggttfvvlr qieplsrryh eparerhphv gqashgsadv rishapatas rgdvllvepv
     1021 rqrpygkrfq vvtttepstt rdvakkinfs yhpiidyitr
//
LOCUS       XP_077504605            1043 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114620 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504605
VERSION     XP_077504605.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648479.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1043
                     /product="uncharacterized protein LOC144114620 isoform X2"
                     /calculated_mol_wt=116587
     Region          669..757
                     /region_name="Spaetzle"
                     /note="pfam16077"
                     /db_xref="CDD:465007"
     CDS             1..1043
                     /gene="LOC144114620"
                     /coded_by="XM_077648479.1:670..3801"
                     /db_xref="GeneID:144114620"
ORIGIN      
        1 mlrrkrfyer ypaavvvvfl slnsisgtaa qvdrsnrepl dnanripwpv yqhstassip
       61 glvpnrppep pkqrlrlsds plppsfikvi nsvsspdwet ttpagpravv lttstttttt
      121 ttttpappps ttaplptssa yykrgqppev dplspsawka vpeippfpkr arlqakrrrk
      181 mrpkqetttk ertlftteva tevpttaypt rkfgvtrrql pslvrgyrsk ptkttppttt
      241 parrafvrpa lpgrkyspvi prrpvltttt ttstsstsss ttttttstrr pfvfgerkvp
      301 swygqtsttt tstttnrpll stpfsrlgrr pnlryksddi spgfgprprl plrtlppvir
      361 davpqtvekd elelsqsvqt qngrrrlvnd vparvhvvkg qlpdipavqi anngteavky
      421 egrhgasdeg gevvrfvaip hfvegsvpar radfkegarv pfrrifrnpf vvpvrrrfpy
      481 agpvavypps lssiedgssq deeviripvq rpesgafaal ppppglplrv vrpaypgavg
      541 pqagaalppa pptnnavqep ayapprppms lgsailpsrv srpkpeeprc drfsedicld
      601 dfeyptaail dsisrqrarf dtmyaevrdr saqvdgvtrk qeerytprhy fgaserdsrp
      661 rdyapdggfl cpseilyerp rrarnhlgvw kvivnigdyr qtmrlekclr pnrpcsyvle
      721 kyssscsqvy syqrlltfek gkglhidifr vpsgcschvk gfspfqrhde pitpvrqqqp
      781 yeydrttyts atslaaakep ssggrqvnnt lwiilgngke ldpetkerlv qqlrqypqla
      841 eqlpqgeavr elvneadgyq rpyarpeqtr ppsygvhysg hktreerphl vnsgavpprw
      901 aqqgpavaae plkyvqpnri rrppppppsd yeteealppg arpryyqkpf kapeedalft
      961 ryheparerh phvgqashgs advrishapa tasrgdvllv epvrqrpygk rfqvvtttep
     1021 sttrdvakki nfsyhpiidy itr
//
LOCUS       XP_077504606            1062 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114620 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504606
VERSION     XP_077504606.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648480.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1062
                     /product="uncharacterized protein LOC144114620 isoform X3"
                     /calculated_mol_wt=118673
     Region          669..757
                     /region_name="Spaetzle"
                     /note="pfam16077"
                     /db_xref="CDD:465007"
     CDS             1..1062
                     /gene="LOC144114620"
                     /coded_by="XM_077648480.1:145..3333"
                     /db_xref="GeneID:144114620"
ORIGIN      
        1 mlrrkrfyer ypaavvvvfl slnsisgtaa qvdrsnrepl dnanripwpv yqhstassip
       61 glvpnrppep pkqrlrlsds plppsfikvi nsvsspdwet ttpagpravv lttstttttt
      121 ttttpappps ttaplptssa yykrgqppev dplspsawka vpeippfpkr arlqakrrrk
      181 mrpkqetttk ertlftteva tevpttaypt rkfgvtrrql pslvrgyrsk ptkttppttt
      241 parrafvrpa lpgrkyspvi prrpvltttt ttstsstsss ttttttstrr pfvfgerkvp
      301 swygqtsttt tstttnrpll stpfsrlgrr pnlryksddi spgfgprprl plrtlppvir
      361 davpqtvekd elelsqsvqt qngrrrlvnd vparvhvvkg qlpdipavqi anngteavky
      421 egrhgasdeg gevvrfvaip hfvegsvpar radfkegarv pfrrifrnpf vvpvrrrfpy
      481 agpvavypps lssiedgssq deeviripvq rpesgafaal ppppglplrv vrpaypgavg
      541 pqagaalppa pptnnavqep ayapprppms lgsailpsrv srpkpeeprc drfsedicld
      601 dfeyptaail dsisrqrarf dtmyaevrdr saqvdgvtrk qeerytprhy fgaserdsrp
      661 rdyapdggfl cpseilyerp rrarnhlgvw kvivnigdyr qtmrlekclr pnrpcsyvle
      721 kyssscsqvy syqrlltfek gkglhidifr vpsgcschvk gfspfqrhde pitpvrqqqp
      781 yeydrttyts atslaaakep ssggrqvnnt lwiilgngke ldpetkerlv qqlrqypqla
      841 eqlpqgeavr elvneadgyq rpyarpeqtr ppsygvhysg hktreerphl vnsgavpprw
      901 aqqgpavaae plkyvqpnri rrppppppsd yeteealppg arpryyqkpf kapeedalft
      961 rssggttfvv lrqieplsrr yheparerhp hvgqashgsa dvrishapat asrgdvllve
     1021 pvrqrpygkr fqvvttteps ttrdvakkin fsyhpiidyi tr
//
LOCUS       XP_077504607            1891 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114621 [Amblyomma americanum].
ACCESSION   XP_077504607
VERSION     XP_077504607.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648481.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1891
                     /product="uncharacterized protein LOC144114621"
                     /calculated_mol_wt=194560
     CDS             1..1891
                     /gene="LOC144114621"
                     /coded_by="XM_077648481.1:153..5828"
                     /db_xref="GeneID:144114621"
ORIGIN      
        1 miphataval iicllavvga epqhdagesr reedvslrla rfveslyedl eeaaqtnvdk
       61 pfpshkafhe fhsqpvrpfq qgrppggggt ispppsrpap gppsqpvipp gpttpprrpp
      121 gtalfgepfe ppkgpqkppa psrgtggygr pegpllppsn vlepeivgpv leyeppdlpq
      181 rppvfsgypi ntidlppppp rqpprprppp qpaappppkf vgvlipadra pglprppvip
      241 papapprppv arppaprppt prppappisr vpapppppfv rppsgiggis fdvqtgvsrp
      301 gpgqppagpg tpgegfgyrp pkrpppgpps pglgvapfpq ppriptgppp gagaprppsp
      361 prpvapspfp vprppgppps pprgvalfpe grplptpqpp rpglfpvpgt pqpptpprqp
      421 papprlpaps pppgfpqpgi gpapprppgp ptpglpfpps pgrppsppqr pvtggrkpsd
      481 iphhkfhefl vtgpkpprpg gglfpgggpg pgpagpqprp ptptpggpfv ppspptfpip
      541 spprpgappp gppppgpgrp prppsppsfp ipapprpgap pfgpppvgpg rpgapppgpg
      601 rpprppspps fpipapprpg appqrppgfp sppppggppp sppefpdtpg ypsrpsrprp
      661 rpppgvalyp egrlperppt gpgrvpapsp psrrpppigt glfpappgvp appapppgip
      721 pafpgpgprp gprplpppgp prpptiplpg pgpfptipsg pprlptppap rpprpptqpp
      781 gpprpppfqp gpfpgppkpp idedgfpetp gypskpsrrp ppqpprgtai fgepgipppp
      841 rrpppppspr ppspplgpsp frpqqpgptp pqprpsprpv pgptppfpgp fpprpqpppg
      901 ppgipgippq iprppsppqv pgrapapspp rptqpggrpk psvhdiphhk fheflaqpvk
      961 pgtgagqvpg iggyparptr prppppsvpp prpqpptpgr psgpptpqgp ppptfpgppp
     1021 prppspplgp psqrfpppsf ppgppsgpfa ppppsrpptp lppgpqrpsp gpppqqrppp
     1081 gvalfpeaps yggrprrprp rpsppaggpp qfpqgrppap ptpspvprpq vpgpqppprf
     1141 pqpgqqpppg prppfgpppp paprpfvpsp pppaprpptg pvpplgprpt pappgprpfp
     1201 gpprpqpfpg pppavgpipg pprpppgprp tplpsprptp rptfpapqfp tgpipappqp
     1261 pgpgagdfpe apgygarrpr prpppgialy pegvtpgrpt pgpapprpsp rpqprpqvpa
     1321 gptpfptprp pprppvppqp pripappppp prpgappppa fpqppsgpgp qprppsppsp
     1381 trpglparpp gqkpsvfdip hskfhefhqq pvrprpgpsp ptpgvalype pgaprppgpp
     1441 rvpspptpsq pfpsprlptp tppsprppvg ptpgprppay vppaqprppp aprppgptpr
     1501 ppayvppgpp rpapptrppt prppsyvppp qpppaprppa prpppsprps ptprpptyvp
     1561 ptpsprpqpp fprpppgptr fpgptppppt psrppvppgp pripsplpps pgvvtpppyr
     1621 psrpvppgvt psfpapppvi prpsplppqp fppprppvtp gvprppptpa pspppgppds
     1681 pgygskrptr prppatggrp fppgplppvp pplpdvpeps rpeipqpapq pfpevpgygq
     1741 grprpqprpq prpqppvpap pvrpqrptet kpqvpprrpp gtalfgepge ppsprprpvp
     1801 apgpqppape pfpeqpgygs krprpgttps gpgprpgggg lfpgsqttpr psgpggrpgd
     1861 tgegrqpvrf pshkafhefh napirirnnv g
//
LOCUS       XP_077504608             381 aa            linear   INV 25-NOV-2025
DEFINITION  tachykinin-like peptides receptor 99D [Amblyomma americanum].
ACCESSION   XP_077504608
VERSION     XP_077504608.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648482.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..381
                     /product="tachykinin-like peptides receptor 99D"
                     /calculated_mol_wt=42928
     Region          60..349
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          62..86
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320515"
     Region          95..117
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320515"
     Region          133..155
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320515"
     Region          177..193
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320515"
     Region          226..249
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320515"
     Region          280..302
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320515"
     Region          317..342
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320515"
     CDS             1..381
                     /gene="LOC144114622"
                     /coded_by="XM_077648482.1:136..1281"
                     /db_xref="GeneID:144114622"
ORIGIN      
        1 mavmevvqyy elgtlnltdg vdnstvlqvs vmanvsgeay atnssdtfss lyevpldivv
       61 llsfcyglvs fvsilgnvlv lwivassrrm qtvtnlfian lavadviigv fsipfqfqaa
      121 llqrwalpyf mcafcpyvqv vsvnvsvftl taiaveryra itsplkarfc skatakllvl
      181 viwlsslvva spnalalrvv lvvdkisglr erpfcvntgm rpftwkaynh alvcaqyflp
      241 lgvicytygk iwkklrqsra pgnmetvrda nivrnknkvi kmmvivvvtf alcwlpyqty
      301 nllaeiypai nsyryinviw fcmhwlamsn scynpfiyai ysekfsaefk armscclkrk
      361 fedsaymass iisqvsfrik y
//
LOCUS       XP_077504609             526 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome P450 4V2-like [Amblyomma americanum].
ACCESSION   XP_077504609
VERSION     XP_077504609.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648483.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..526
                     /product="cytochrome P450 4V2-like"
                     /calculated_mol_wt=59999
     Region          106..515
                     /region_name="cytochrome_P450"
                     /note="cytochrome P450 (CYP) superfamily; cl41757"
                     /db_xref="CDD:477761"
     Site            order(156,160,167,212,326..327,330..331,334..335,338,391,
                     400,402,425,459..461,465..469,472..473)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410651"
     Site            order(238..239,326,329..330,334,398..401)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410651"
     CDS             1..526
                     /gene="LOC144114623"
                     /coded_by="XM_077648483.1:298..1878"
                     /db_xref="GeneID:144114623"
ORIGIN      
        1 mistltalrt ferapgllyw algavastia aaliiyvarg maewlrmwwh lrdvpqpkpq
       61 ksftmlldly kqlsamdprl dmkvkafkyl hnlfksvedq dvtvawygpy afllgatppv
      121 ieavlghrkn inkaffydmt kpwigggiln lsdapwktrr raitpafhfg ilddyvpimn
      181 krgerlmrkv atmngeffdi lpivraaalg vlletsmgid yneeeiervg ylkihdaisd
      241 sivnrvihfh hwfdflylfs sehkemlknv eaakqfvtgi lkkrfadyrk girdpiskns
      301 fleilirmcv degtmseidv rdellstlig gfdttattla ytlyllghhp eiqakvhdei
      361 dlvcgddwdk pisaedlknl tytecvvkes mrlypaasmi graldtdikv gkytiprgtv
      421 avaalyflhr hpryhkdpda fkperfidtk gmhtyaftpf sagprnclgq kfamkedkil
      481 lvhilrrfqv tskvpiedll lamelilkpm qgleikltpr khpsss
//
LOCUS       XP_077504610             512 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome P450 4V2-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504610
VERSION     XP_077504610.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648484.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..512
                     /product="cytochrome P450 4V2-like isoform X1"
                     /calculated_mol_wt=59005
     Region          92..501
                     /region_name="CYP4"
                     /note="cytochrome P450 family 4; cd20628"
                     /db_xref="CDD:410721"
     Site            order(117,134..135,142,146,153,313,316..317,320,382,386,
                     445..447,451..455,458..459,463)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410721"
     Site            order(135,315..316,320,385,387)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410721"
     CDS             1..512
                     /gene="LOC144114624"
                     /coded_by="XM_077648484.1:234..1772"
                     /db_xref="GeneID:144114624"
ORIGIN      
        1 mqyvliatkl aalialvtii ffigkqalkw frmyyylrhl phveerwpfs lfldtwtalr
       61 rmdrhlpita klfnymqeit skiyeqevtv ayygpqpfvi gisptlfeai lsstvnlnks
      121 fiyrmmkpwm gngvltsdke lwrtrrkilt pafhfktlrn yvpimnrrtn hvveklmkke
      181 atafdilpvl rqaafgmlfe tamgvninee evaskrllct tdelaasiis rvlnvflwpd
      241 avfnmtqegk tflrkvqyir dynkkiiqqr kaeyvsggtg tesrksfldi llrmhidegt
      301 ltehevaeev atffiggfdt tataasytly llghhtdvqk kvhaeidaif gydkqrgvti
      361 edinqlkyle cvikesmrly ppvplvarni dedvhvgshi ipkgtvvlca iyflhrhpgv
      421 yadansfipe rfmdnrnisp yayvpfsags rncigqkfaq leekillaqi lrnfhvesvv
      481 piedlklqle ivlrplqgiq vrltprhrtg sr
//
LOCUS       XP_077504611             454 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome P450 4V2-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504611
VERSION     XP_077504611.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648485.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..454
                     /product="cytochrome P450 4V2-like isoform X2"
                     /calculated_mol_wt=52480
     Region          92..443
                     /region_name="cytochrome_P450"
                     /note="cytochrome P450 (CYP) superfamily; cl41757"
                     /db_xref="CDD:477761"
     Site            order(142,146,153,198,312..313,319,328,330,353,387..389,
                     393..397,400..401)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410651"
     CDS             1..454
                     /gene="LOC144114624"
                     /coded_by="XM_077648485.1:234..1598"
                     /db_xref="GeneID:144114624"
ORIGIN      
        1 mqyvliatkl aalialvtii ffigkqalkw frmyyylrhl phveerwpfs lfldtwtalr
       61 rmdrhlpita klfnymqeit skiyeqevtv ayygpqpfvi gisptlfeai lsstvnlnks
      121 fiyrmmkpwm gngvltsdke lwrtrrkilt pafhfktlrn yvpimnrrtn hvveklmkke
      181 atafdilpvl rqaafgmlfe tamgvninee evaskrllct tdelaasiis rvlnvflwpd
      241 avfnmtqegk tflrkvqyir dynkkiiqqr kaeyvsggtg tesrksfldi llrmhidegt
      301 ltehevaeev atffigesmr lyppvplvar nidedvhvgs hiipkgtvvl caiyflhrhp
      361 gvyadansfi perfmdnrni spyayvpfsa gsrncigqkf aqleekilla qilrnfhves
      421 vvpiedlklq leivlrplqg iqvrltprhr tgsr
//
LOCUS       XP_077504612             550 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome P450 4c3-like [Amblyomma americanum].
ACCESSION   XP_077504612
VERSION     XP_077504612.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648486.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..550
                     /product="cytochrome P450 4c3-like"
                     /calculated_mol_wt=63178
     Region          106..541
                     /region_name="CYP4"
                     /note="cytochrome P450 family 4; cd20628"
                     /db_xref="CDD:410721"
     Site            order(136,153..154,161,165,172,351,354..355,358,420,424,
                     485..487,491..495,498..499,503)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410721"
     Site            order(154,353..354,358,423,425)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410721"
     CDS             1..550
                     /gene="LOC144114626"
                     /coded_by="XM_077648486.1:203..1855"
                     /db_xref="GeneID:144114626"
ORIGIN      
        1 mevtlvhifs qmlgtastml asllwyclaa avgvfvclvg lllyikrkql etlvymekfp
       61 grtepipmlt twsihrtlsk eahrldmgth lfqlavgfsr iyqkqgmlrf ydathpvlav
      121 fkagpveell tsntilkkgq eydmigpwla tglltssgsk wrsrrklltr afhfriledf
      181 lsaineqskv lvrklgqlgk dgscdivplv tlcaldiice timgytinaq snshseyvqa
      241 iqvlghlftl rlesplywvd aifristpgr lfhskirelh rftmkvirer keellscpel
      301 qaaieeapea sdayglrgkv rkpfldillr ehikdpenfr eedvreevdt fmfeghdtta
      361 mgmswalyli glyqehqeli rreldaifgs drnrpitsed lkqmkymecc lkesqrlyps
      421 vpfitrnceq dvtiagrklp kgadvqigiy nlhrdpeffp kpeefiperf fpenvkgrhp
      481 fafipfsagp rncigqrfam meekvviani lrnyklvslh hrdkirvmae lvlrpmnglh
      541 vkfiprtpsv
//
LOCUS       XP_077504613             540 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome P450 4c3-like [Amblyomma americanum].
ACCESSION   XP_077504613
VERSION     XP_077504613.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648487.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..540
                     /product="cytochrome P450 4c3-like"
                     /calculated_mol_wt=62752
     Region          103..534
                     /region_name="CYP4"
                     /note="cytochrome P450 family 4; cd20628"
                     /db_xref="CDD:410721"
     Site            order(131,148..149,156,160,167,344,347..348,351,413,417,
                     478..480,484..488,491..492,496)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410721"
     Site            order(149,346..347,351,416,418)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410721"
     CDS             1..540
                     /gene="LOC144114627"
                     /coded_by="XM_077648487.1:198..1820"
                     /db_xref="GeneID:144114627"
ORIGIN      
        1 mdlslaslpy ffhwtalkiv talsatllgi vgalvavvyy rwkrthvhlk hlpgiedewp
       61 firniithwh mkrrekehdl dynvlffeyl nagsqrmkhl pyfraymgev pyllihkaea
      121 aeaifssptm leksieytwl hswlgtgllt stgtkwktrr rmltpafhfr iledflpimn
      181 ehsvvfkdli kehvgkpdgv divpsvarct ldvicetamg tcvnaqrcsd skyvravlrl
      241 gelwfsratk pwtwpnfmyr ftadsvefsq cvntvhtftr kvikdrkkml lelqaasnas
      301 ngageadfvn msgcrrafld lllsqhfkdp sfteediree vdtftfeghd ttavgiswtl
      361 yllglhqdvq qkvyeeleei fgddkdrdat medirrmkyl ecaikeaqrl fpsvplvgrl
      421 lqedwtydgy iipkgtvctv fiymlhrdpk efpkpeeyip erflpencvg rhpfayvpfs
      481 agyrncigqk fasmemktlv srvlrnykle smhhrdkvqt vselvlrarn glrirltsre
//
LOCUS       XP_077504615             530 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome P450 4V2-like [Amblyomma americanum].
ACCESSION   XP_077504615
VERSION     XP_077504615.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648489.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..530
                     /product="cytochrome P450 4V2-like"
                     /calculated_mol_wt=61202
     Region          82..521
                     /region_name="CYP4"
                     /note="cytochrome P450 family 4; cd20628"
                     /db_xref="CDD:410721"
     Site            order(113,130..131,138,142,149,331,334..335,338,400,404,
                     465..467,471..475,478..479,483)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410721"
     Site            order(131,333..334,338,403,405)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410721"
     CDS             1..530
                     /gene="LOC144114628"
                     /coded_by="XM_077648489.1:182..1774"
                     /db_xref="GeneID:144114628"
ORIGIN      
        1 mlfvvyalag alslvvgall avhlarhrtw ryvklmpgpn emipmrtvlq iqmrasamkd
       61 rfppnvvfmq srfaltleyq kegffafyig trpcitvyka ehvetfmtnr stqsksfhyr
      121 llhswlgtgl ltsagpkwkt rrrmltpafh fkiledfvap mnrmarqtve kvkkqlnepw
      181 idavplaasc aldvlletim gvsnskkdce chdyvknvkf vaerivqrsq tpwlsfdwvy
      241 crtedgrqhr kheslihaft kkviakrree lmeeinsdni akeatpkiee nlelctgkrk
      301 rltfidillr ysiqvdsslt dddireevdt fmfeghdtta msiawslyli alhpdiqnkv
      361 qeeldgmldd dlevdirleq lkelkyfdrv lkecqrlfpp vpmigrsven dvqigkytvp
      421 kgsdvdvfly alhrdpsvfp epevfdpdrf lpenvvrrhp fayvpfsagp rncigqkyas
      481 mevkiviati lrhfllkavd qrdqliltce lvlrpvnglr vafaprsrct
//
LOCUS       XP_077504616             521 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome P450 4c3-like [Amblyomma americanum].
ACCESSION   XP_077504616
VERSION     XP_077504616.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648490.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..521
                     /product="cytochrome P450 4c3-like"
                     /calculated_mol_wt=60120
     Region          101..511
                     /region_name="CYP4"
                     /note="cytochrome P450 family 4; cd20628"
                     /db_xref="CDD:410721"
     Site            order(126,143..144,151,155,162,322,325..326,329,391,395,
                     455..457,461..465,468..469,473)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410721"
     Site            order(144,324..325,329,394,396)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410721"
     CDS             1..521
                     /gene="LOC144114629"
                     /coded_by="XM_077648490.1:143..1708"
                     /db_xref="GeneID:144114629"
ORIGIN      
        1 mepipavyrp llpskivtaf isvaaigvay ycvrkvarwi rvyaslrnap rpkeslpfcl
       61 ildmwrqist mdsslevpar lfnyfktifs evldhdltva yygpkpflva vtpntvervl
      121 snsenvnksf lyamlkswvg nglltsekti wkkrrkvltp afhfrildey vpimnrrasv
      181 lceklavlgr dhfdllpvmr latfgilfet amgitldeed vkktgflkvn deiatdiitr
      241 mmnihhwpef iyrrtgvgqr yyqkvelikk ytrdildcrk ksykiegera vqkksfmdil
      301 lrmhmeegvf teeeireevn tfmiggfdtt ataasfaihl lgnhpetlak vheeldavfg
      361 sdrerpvtve dikqlkyldc vlkealrlyp pipaiarklg edvsigkyti pkgtvsvifi
      421 yfmhrhprff pkpndfiper flevdqdrhp flyipfagga rncigqkfaq ledkillaqi
      481 lrrfkvkski rnedlqmsle lvlrpsqglh lkltprdhav t
//
LOCUS       XP_077504617             532 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome P450 4C1-like [Amblyomma americanum].
ACCESSION   XP_077504617
VERSION     XP_077504617.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648491.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..532
                     /product="cytochrome P450 4C1-like"
                     /calculated_mol_wt=61254
     Region          98..524
                     /region_name="CYP4"
                     /note="cytochrome P450 family 4; cd20628"
                     /db_xref="CDD:410721"
     Site            order(129,146..147,154,158,165,331,334..335,338,400,404,
                     465..467,471..475,478..479,483)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410721"
     Site            order(147,333..334,338,403,405)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410721"
     CDS             1..532
                     /gene="LOC144114632"
                     /coded_by="XM_077648491.1:1..1599"
                     /db_xref="GeneID:144114632"
ORIGIN      
        1 mvkeawrslt wslrfgevrv wllylvtvca lvllsnfwwa ryrifrrqmn ilkdmpkdsg
       61 ryiasgiakv lavlrnpmdp avvmyegfng faeltskegi fhfwigmqpf vvlhaahtve
      121 pllsssvnls ktgqyhflgp vlgeglltst gtkwrsrrkl ltpafhfrvl dscvaifneq
      181 arvlvqkire sehmdfvdlf pmmthctldv lcesamgiql gaqgttqhny vknlhtllns
      241 gmerlckpwl wwnvaynlss qrrrfsrsae glnkfvkevi rrrkvilsqd kssrqdqnsk
      301 yskafldvll khhledssls eediieevnt flfaghetta vgltyllyil glhqdvqrkv
      361 teeidlifhg dfdrdvtred tsamkyldcv mkesqrifti lpiygrevee dfqigryvvp
      421 rgstciilgq tlhkdrayfp npevfdpdrf lpenskgrhp yaflpfsagp rncvgqkfam
      481 teekvvvatv lrhfhitsld prdrlilapq pilhprsvsg gvrvkftprk rr
//
LOCUS       XP_077504618             522 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114633 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504618
VERSION     XP_077504618.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648492.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..522
                     /product="uncharacterized protein LOC144114633 isoform X1"
                     /calculated_mol_wt=59151
     CDS             1..522
                     /gene="LOC144114633"
                     /coded_by="XM_077648492.1:241..1809"
                     /db_xref="GeneID:144114633"
ORIGIN      
        1 mwlllmrsct sqddfrmkrg lvsscidlfv sfgkiaamel eqrisnwlpa ldkeiveltv
       61 qklqqcgves lehlkyvvee dlqflkpisr rillerfhsa atpnppvsls pndiqsptst
      121 qcasfstpwe vfppqlmkac qegkrpvksd lnemvrlvsd hilainrkpg rkilrviaae
      181 ivshypktfe dtlngavigs gietllwkle ncvnnkrrps seqphqfepd deldlsvkrv
      241 kiqrdsygcv awqpklpsde tadsqekkkd dllsqfrlaf pdetmvaqlm setyasqrql
      301 inasknirdi erswpflfqa khltnhvnil lgsnvaktfd drlktvgrqv fryahsqvkk
      361 ecvksclqei eaaktnrksm aveyeampll llaifgedke lfyklteema sdddlgdlps
      421 cpficakgwt fltansytic vdthpvlhas kpdeafkllf fahfafniqy qketslclef
      481 tqraiaginp argtkvqkng gkqhclsprv aalatalkdy df
//
LOCUS       XP_077504619             485 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114633 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504619
VERSION     XP_077504619.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648493.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..485
                     /product="uncharacterized protein LOC144114633 isoform X2"
                     /calculated_mol_wt=54942
     CDS             1..485
                     /gene="LOC144114633"
                     /coded_by="XM_077648493.1:2793..4250"
                     /db_xref="GeneID:144114633"
ORIGIN      
        1 meleqrisnw lpaldkeive ltvqklqqcg veslehlkyv veedlqflkp isrrillerf
       61 hsaatpnppv slspndiqsp tstqcasfst pwevfppqlm kacqegkrpv ksdlnemvrl
      121 vsdhilainr kpgrkilrvi aaeivshypk tfedtlngav igsgietllw klencvnnkr
      181 rpsseqphqf epddeldlsv krvkiqrdsy gcvawqpklp sdetadsqek kkddllsqfr
      241 lafpdetmva qlmsetyasq rqlinaskni rdierswpfl fqakhltnhv nillgsnvak
      301 tfddrlktvg rqvfryahsq vkkecvkscl qeieaaktnr ksmaveyeam pllllaifge
      361 dkelfyklte emasdddlgd lpscpficak gwtfltansy ticvdthpvl haskpdeafk
      421 llffahfafn iqyqketslc leftqraiag inpargtkvq knggkqhcls prvaalatal
      481 kdydf
//
LOCUS       XP_077504620            1747 aa            linear   INV 25-NOV-2025
DEFINITION  protein qui-1 [Amblyomma americanum].
ACCESSION   XP_077504620
VERSION     XP_077504620.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648494.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1747
                     /product="protein qui-1"
                     /calculated_mol_wt=190778
     Region          407..>515
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Region          881..1332
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          961..998
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1004..1040
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1045..1081
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1092..1523
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          1129..1164
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1171..1214
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1220..1257
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1263..1299
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1304..1357
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1385..1420
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1426..1463
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1469..1495
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..1747
                     /gene="LOC144114635"
                     /coded_by="XM_077648494.1:533..5776"
                     /db_xref="GeneID:144114635"
ORIGIN      
        1 macsggasly qdhilqavlq gnltsvpepp srllkilvas srpdfeherr vvqenvlpel
       61 qrhcaslgvd velldlqqgl dprlrpaaat lreledchar slgcflicmv gnkyrpfalp
      121 dhvdrtvfep ihqaaqdagl dvsllgqwyl lnenllpaay elqppadrah kadggpddgl
      181 sasvleeqeq vvkvlqygak valdeglida dterqfrmsg vhalldyalk lskmaqhkii
      241 cvvrqfdgin mndtlastha dvsaqgggcs deetatyted lvadiilnve rknvhfysvp
      301 whasgldtel sahssylekl agallttikg mvdeasqvet pdwdelpptf rkvvqevvre
      361 sqthlcnsrq llrclgvsqs ldsdygpllq iqqlmladee rvrhtpiivc gregcgkstl
      421 lsqvlmycpe wlgcdvvriv rgvgqspcca ypaemlrnlc lhismvfgfe ispkhqsfel
      481 sklstwfqel lkrveastsd lvivlddldr lrgpahhqaa tlgwlpwnlp lnvhlvcsva
      541 qeadavlgll rsripagdsy vlippladaa smlqshlkdn krlltrqqwd vlrqrlggqs
      601 atplyvtlls qqamrwasye vldgelhvpa dtgafvsrsl erierqfgla pvkkiasylt
      661 ctsyglrepe viellsstec dgtelaavsw lafkkelgvl lkesyvdcrs ylqwshrcig
      721 tcvrkrylse raeaiachad lanafhlgfl mqkeekgpaf ddsnkskrke dwsdllrevd
      781 elwqhlfhsg dskrlnqdav cnfefllsai rgasisyvks vleivraqll dweiellynm
      841 tkqsvdvlsq dpqqlateil nwlrpyadgn skvladlvsq asswcsqakt pllvpltswl
      901 slalppqvls ltlsapirql vaapdsqhvf cateaddklv svyhltskkl vrqmsghkaa
      961 isclhmsasg lhllsgsedt dvlvwnpatg elrrrlshhv agvlcvactn aedlvlsgse
     1021 igvvvvarld tgqlvqrlen hrgvvsavav nrgddifata ssdctvciwc letftllntl
     1081 slpaaverls isgdstflll gcadqcvrvr slttgsdvhc lqgyagrvsa lafardncrc
     1141 vlgtrdgkaf ifdvhsarlv qtltahpdsa vvaiqaqsrd tflitaagnk ivvwnfylkk
     1201 semchpqpks kkvdvhkdpi scvalsrdgs vavtgsrngq lkvwqmstge ahtnldghta
     1261 aitclafapn glfvvsgsed ntlrvwgltl glvvstfkeh qskivsvfvt sdsrrvlsvd
     1321 aqglhrlwta dsatqliaca ktskqvtlha nvvftvggks dnslkywpvf etdsektvsh
     1381 sdailcytvt ydcqiivtgs qdmslkvwev atakltqvlv ghegpvtcva vapfrgslav
     1441 sgsqdcnliv wdvttgsdrf vlrghteair aakltldgsv avsssddntl qlwntqnghr
     1501 vasfdlhatv lavttslntg hlvvqlasst lvpmlrllnn pgkglmldlp pgtpvgdepk
     1561 tlgghplrgv vpkrvllrgn lkreqsfdsf ywdlraqspk hetsvddfra gkplspfgsr
     1621 ehlqltggaa vwngglgrgp rsaaaphagg aadlglqpkp klpkhkilkk qqsmfacfpe
     1681 ftqqtpptll spvapkelve kkelaksgra tavllprtns veeapepaar dpgpentvtg
     1741 savcalm
//
LOCUS       XP_077504621             337 aa            linear   INV 25-NOV-2025
DEFINITION  replication factor C subunit RfC4 [Amblyomma americanum].
ACCESSION   XP_077504621
VERSION     XP_077504621.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648495.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..337
                     /product="replication factor C subunit RfC4"
                     /calculated_mol_wt=37750
     Region          19..336
                     /region_name="PLN03025"
                     /note="replication factor C subunit; Provisional"
                     /db_xref="CDD:178596"
     CDS             1..337
                     /gene="RfC4"
                     /coded_by="XM_077648495.1:149..1162"
                     /db_xref="GeneID:144114636"
ORIGIN      
        1 maeamdvdse saqkkkdcip wvekyrpakf seivgneeti arlevfsreg nvpnvilsgp
       61 pgvgktttil clarillgpa freavlelna sndrgidvvr nkikmfaqkk vtlalgkhki
      121 iildeadsmt egaqqalrrt meiyskttrf alacntsdki iepiqsrcav vrfsrltdaq
      181 ilaklldice rentsyaedg lealvftaqg dmrqainnlq stvvgfghvn sqnvfkvcde
      241 phpeqikdml qhcvdgqldn aykimlhmwk lgysaediiv nifrvckshd mredvklefi
      301 keigytqmrt lqgvhsllqm agllarlcmk nrqckad
//
LOCUS       XP_077504622             641 aa            linear   INV 25-NOV-2025
DEFINITION  actin-related protein 5 [Amblyomma americanum].
ACCESSION   XP_077504622
VERSION     XP_077504622.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648496.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..641
                     /product="actin-related protein 5"
                     /calculated_mol_wt=73086
     Region          32..>272
                     /region_name="ASKHA_NBD_Arp5"
                     /note="nucleotide-binding domain (NBD) of actin-related
                     protein5 (Arp5) and similar proteins; cd10211"
                     /db_xref="CDD:466817"
     Region          <303..>440
                     /region_name="PRK03918"
                     /note="DNA double-strand break repair ATPase Rad50"
                     /db_xref="CDD:235175"
     Region          <482..616
                     /region_name="ASKHA_ATPase-like"
                     /note="ATPase-like domain of the ASKHA (Acetate and Sugar
                     Kinases/Hsc70/Actin) superfamily; cl49607"
                     /db_xref="CDD:483947"
     CDS             1..641
                     /gene="Arp5"
                     /coded_by="XM_077648496.1:568..2493"
                     /db_xref="GeneID:144114637"
ORIGIN      
        1 mssqdvytip nlakepdsff tysdalrlrk tpvvidngsy ecrvgwasde aprmvfknit
       61 akqrgkkese vqvgndimni eavrwllktq fdrnvvthfd iqehifdhif shlgistenr
      121 vdhpivmtev vgnpnyyrqn msellfehyh vpqvlygida lfslhhncsk kaenalvlsf
      181 ghhtthiipv lncqidienc krislggnni taylqrllql khphltaavt lsrtehlmhq
      241 hcyvaenyme elkkwadvdy fnehcqlvql pynttssgai gsgvgkmdic fnivkrvqes
      301 iakkkqdtla ddeeklqqli niqellefed eatfqrtlqe lgfssieels lvinelqrqv
      361 qqvknslasg naadpdlmes alkrscsdld prsfsepdtw isavrkrree lkekkmlqqq
      421 rflerahnag qkgksrkkes qfldsefdda dyaeegeeme eiiaeferef kkkqlgadqv
      481 gtsiaeyyql qlgterirap eilfqpsiig veqagvaetl efvfsrytke qqsflaqnvf
      541 vtgspallpg icerlctell amrpfqspfs vtfaknpald swkgarqcav qrdflnkfsi
      601 kradyeekgp dylkehcysn vssspvvqsp akaltmqsst l
//
LOCUS       XP_077504623             612 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114638 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504623
VERSION     XP_077504623.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648497.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..612
                     /product="uncharacterized protein LOC144114638 isoform X1"
                     /calculated_mol_wt=70160
     Region          5..604
                     /region_name="COG4805"
                     /note="Uncharacterized conserved protein, DUF885 family
                     [Function unknown]"
                     /db_xref="CDD:443833"
     CDS             1..612
                     /gene="LOC144114638"
                     /coded_by="XM_077648497.1:62..1900"
                     /db_xref="GeneID:144114638"
ORIGIN      
        1 mpafmravfa aacglmlati spyvykaivg yysdrpeama aqlfeefwqw rledspefat
       61 mvgfhgydhl lesyslqsfr drrdkvqyfl kkakelpkte dqwlmsslni liaglegylr
      121 gldtdghlyp isfiegvhvd ifqtidymvf ntvddfrkll sryeklpkql eeivdlmkeg
      181 irlkktmhav smkgvldrln sfegpaekse ffkrfekypd aiseqerevl kqdarkviea
      241 svlpafkqlk kfiqneymph lrheiaatsl pngeayykec lqyhleldln plevhqlgln
      301 everissamk qiikslgsea spkefsealr ndssflckss kevvqiyeql ldeeinpkll
      361 tifpsvpksp lrvqlqdask ssgpcamyiq gtpdfsrpgi fyvnaadlsr spkylmptla
      421 lhegnpghhl qgsyamelpg mppfrsnied rryndgpvhf plhtayvegw glyseylgye
      481 lgvyktlhek fghltqemlr acrlvvdtgm halgwsreka vqyllehtss slgeveseid
      541 ryitwpgqac aykvgelkik qlrkkaedil gvkfdvrafh kvvldnpgal thlekqvdky
      601 ieetrknqgr el
//
LOCUS       XP_077504624             574 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114638 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504624
VERSION     XP_077504624.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648498.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..574
                     /product="uncharacterized protein LOC144114638 isoform X2"
                     /calculated_mol_wt=66032
     Region          2..566
                     /region_name="COG4805"
                     /note="Uncharacterized conserved protein, DUF885 family
                     [Function unknown]"
                     /db_xref="CDD:443833"
     CDS             1..574
                     /gene="LOC144114638"
                     /coded_by="XM_077648498.1:188..1912"
                     /db_xref="GeneID:144114638"
ORIGIN      
        1 maaqlfeefw qwrledspef atmvgfhgyd hllesyslqs frdrrdkvqy flkkakelpk
       61 tedqwlmssl niliaglegy lrgldtdghl ypisfiegvh vdifqtidym vfntvddfrk
      121 llsryeklpk qleeivdlmk egirlkktmh avsmkgvldr lnsfegpaek seffkrfeky
      181 pdaiseqere vlkqdarkvi easvlpafkq lkkfiqneym phlrheiaat slpngeayyk
      241 eclqyhleld lnplevhqlg lneverissa mkqiikslgs easpkefsea lrndssflck
      301 sskevvqiye qlldeeinpk lltifpsvpk splrvqlqda skssgpcamy iqgtpdfsrp
      361 gifyvnaadl srspkylmpt lalhegnpgh hlqgsyamel pgmppfrsni edrryndgpv
      421 hfplhtayve gwglyseylg yelgvyktlh ekfghltqem lracrlvvdt gmhalgwsre
      481 kavqylleht ssslgevese idryitwpgq acaykvgelk ikqlrkkaed ilgvkfdvra
      541 fhkvvldnpg althlekqvd kyieetrknq grel
//
LOCUS       XP_077504625             574 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114638 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504625
VERSION     XP_077504625.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648499.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..574
                     /product="uncharacterized protein LOC144114638 isoform X2"
                     /calculated_mol_wt=66032
     Region          2..566
                     /region_name="COG4805"
                     /note="Uncharacterized conserved protein, DUF885 family
                     [Function unknown]"
                     /db_xref="CDD:443833"
     CDS             1..574
                     /gene="LOC144114638"
                     /coded_by="XM_077648499.1:164..1888"
                     /db_xref="GeneID:144114638"
ORIGIN      
        1 maaqlfeefw qwrledspef atmvgfhgyd hllesyslqs frdrrdkvqy flkkakelpk
       61 tedqwlmssl niliaglegy lrgldtdghl ypisfiegvh vdifqtidym vfntvddfrk
      121 llsryeklpk qleeivdlmk egirlkktmh avsmkgvldr lnsfegpaek seffkrfeky
      181 pdaiseqere vlkqdarkvi easvlpafkq lkkfiqneym phlrheiaat slpngeayyk
      241 eclqyhleld lnplevhqlg lneverissa mkqiikslgs easpkefsea lrndssflck
      301 sskevvqiye qlldeeinpk lltifpsvpk splrvqlqda skssgpcamy iqgtpdfsrp
      361 gifyvnaadl srspkylmpt lalhegnpgh hlqgsyamel pgmppfrsni edrryndgpv
      421 hfplhtayve gwglyseylg yelgvyktlh ekfghltqem lracrlvvdt gmhalgwsre
      481 kavqylleht ssslgevese idryitwpgq acaykvgelk ikqlrkkaed ilgvkfdvra
      541 fhkvvldnpg althlekqvd kyieetrknq grel
//
LOCUS       XP_077504626             574 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114638 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504626
VERSION     XP_077504626.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648500.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..574
                     /product="uncharacterized protein LOC144114638 isoform X2"
                     /calculated_mol_wt=66032
     Region          2..566
                     /region_name="COG4805"
                     /note="Uncharacterized conserved protein, DUF885 family
                     [Function unknown]"
                     /db_xref="CDD:443833"
     CDS             1..574
                     /gene="LOC144114638"
                     /coded_by="XM_077648500.1:56..1780"
                     /db_xref="GeneID:144114638"
ORIGIN      
        1 maaqlfeefw qwrledspef atmvgfhgyd hllesyslqs frdrrdkvqy flkkakelpk
       61 tedqwlmssl niliaglegy lrgldtdghl ypisfiegvh vdifqtidym vfntvddfrk
      121 llsryeklpk qleeivdlmk egirlkktmh avsmkgvldr lnsfegpaek seffkrfeky
      181 pdaiseqere vlkqdarkvi easvlpafkq lkkfiqneym phlrheiaat slpngeayyk
      241 eclqyhleld lnplevhqlg lneverissa mkqiikslgs easpkefsea lrndssflck
      301 sskevvqiye qlldeeinpk lltifpsvpk splrvqlqda skssgpcamy iqgtpdfsrp
      361 gifyvnaadl srspkylmpt lalhegnpgh hlqgsyamel pgmppfrsni edrryndgpv
      421 hfplhtayve gwglyseylg yelgvyktlh ekfghltqem lracrlvvdt gmhalgwsre
      481 kavqylleht ssslgevese idryitwpgq acaykvgelk ikqlrkkaed ilgvkfdvra
      541 fhkvvldnpg althlekqvd kyieetrknq grel
//
LOCUS       XP_077504627             551 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114638 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504627
VERSION     XP_077504627.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648501.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..551
                     /product="uncharacterized protein LOC144114638 isoform X3"
                     /calculated_mol_wt=63088
     Region          28..543
                     /region_name="COG4805"
                     /note="Uncharacterized conserved protein, DUF885 family
                     [Function unknown]"
                     /db_xref="CDD:443833"
     CDS             1..551
                     /gene="LOC144114638"
                     /coded_by="XM_077648501.1:690..2345"
                     /db_xref="GeneID:144114638"
ORIGIN      
        1 mrqseaqfsn hfipvklsth pedkvqyflk kakelpkted qwlmsslnil iaglegylrg
       61 ldtdghlypi sfiegvhvdi fqtidymvfn tvddfrklls ryeklpkqle eivdlmkegi
      121 rlkktmhavs mkgvldrlns fegpaeksef fkrfekypda iseqerevlk qdarkvieas
      181 vlpafkqlkk fiqneymphl rheiaatslp ngeayykecl qyhleldlnp levhqlglne
      241 verissamkq iikslgseas pkefsealrn dssflckssk evvqiyeqll deeinpkllt
      301 ifpsvpkspl rvqlqdasks sgpcamyiqg tpdfsrpgif yvnaadlsrs pkylmptlal
      361 hegnpghhlq gsyamelpgm ppfrsniedr ryndgpvhfp lhtayvegwg lyseylgyel
      421 gvyktlhekf ghltqemlra crlvvdtgmh algwsrekav qyllehtsss lgeveseidr
      481 yitwpgqaca ykvgelkikq lrkkaedilg vkfdvrafhk vvldnpgalt hlekqvdkyi
      541 eetrknqgre l
//
LOCUS       XP_077504628             551 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114638 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504628
VERSION     XP_077504628.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648502.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..551
                     /product="uncharacterized protein LOC144114638 isoform X3"
                     /calculated_mol_wt=63088
     Region          28..543
                     /region_name="COG4805"
                     /note="Uncharacterized conserved protein, DUF885 family
                     [Function unknown]"
                     /db_xref="CDD:443833"
     CDS             1..551
                     /gene="LOC144114638"
                     /coded_by="XM_077648502.1:172..1827"
                     /db_xref="GeneID:144114638"
ORIGIN      
        1 mrqseaqfsn hfipvklsth pedkvqyflk kakelpkted qwlmsslnil iaglegylrg
       61 ldtdghlypi sfiegvhvdi fqtidymvfn tvddfrklls ryeklpkqle eivdlmkegi
      121 rlkktmhavs mkgvldrlns fegpaeksef fkrfekypda iseqerevlk qdarkvieas
      181 vlpafkqlkk fiqneymphl rheiaatslp ngeayykecl qyhleldlnp levhqlglne
      241 verissamkq iikslgseas pkefsealrn dssflckssk evvqiyeqll deeinpkllt
      301 ifpsvpkspl rvqlqdasks sgpcamyiqg tpdfsrpgif yvnaadlsrs pkylmptlal
      361 hegnpghhlq gsyamelpgm ppfrsniedr ryndgpvhfp lhtayvegwg lyseylgyel
      421 gvyktlhekf ghltqemlra crlvvdtgmh algwsrekav qyllehtsss lgeveseidr
      481 yitwpgqaca ykvgelkikq lrkkaedilg vkfdvrafhk vvldnpgalt hlekqvdkyi
      541 eetrknqgre l
//
LOCUS       XP_077504629             463 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114639 [Amblyomma americanum].
ACCESSION   XP_077504629
VERSION     XP_077504629.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648503.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..463
                     /product="uncharacterized protein LOC144114639"
                     /calculated_mol_wt=53317
     Region          284..462
                     /region_name="Peptidase_C48"
                     /note="Ulp1 protease family, C-terminal catalytic domain;
                     pfam02902"
                     /db_xref="CDD:397169"
     CDS             1..463
                     /gene="LOC144114639"
                     /coded_by="XM_077648503.1:232..1623"
                     /db_xref="GeneID:144114639"
ORIGIN      
        1 mvprlnavdq rldtlrasmk afhgmpdlrt ftrrsdvrda tvcaaarwhs nclsterrdw
       61 alwmasvqvr nrtepfykrr crraasanfg gcaaketglf svrslrqkar erarltvtqv
      121 delvcynvvl qnlvdsevkv deetqsldgr qqhpfttktl ceddlklfta qpwelspsrq
      181 dsaypsakpg eqpkiahyvs idsadwyvdl krdvvaacet cdekyeepep ptavpgkfap
      241 pelteamqae veaalkpcpq eevlakgfsl tvkradmmtl senqwindtv infymsmlvn
      301 rsekgslrvy afntffftkl crdghaglkr wtrkidlfsy hivlvpihln rhwflavvdf
      361 rrsqialyds fgaahakakc idllreylee eslhkrnhgi nwddwkfiva edvplqqnms
      421 dcgmftcqfa ecisrdapfq ftqqhmpyyr krvvyeilhs all
//
LOCUS       XP_077504630             412 aa            linear   INV 25-NOV-2025
DEFINITION  procollagen galactosyltransferase 1-A-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504630
VERSION     XP_077504630.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648504.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..412
                     /product="procollagen galactosyltransferase 1-A-like
                     isoform X1"
                     /calculated_mol_wt=46617
     Region          7..>165
                     /region_name="BcsA"
                     /note="Glycosyltransferase, catalytic subunit of cellulose
                     synthase and poly-beta-1,6-N-acetylglucosamine synthase
                     [Cell motility]; COG1215"
                     /db_xref="CDD:440828"
     CDS             1..412
                     /gene="LOC144114641"
                     /coded_by="XM_077648504.1:238..1476"
                     /db_xref="GeneID:144114641"
ORIGIN      
        1 mrylsvmffl cfwlpllssv gvvrcatsdd kleaptllia vvlrnkahvl pqffgyleqq
       61 nypksrlslw iytdhnvdnt aeivnswaee vsndyhnvnv tredgdaffp deeglqkwtp
      121 erywhiirlr eeaihvartl wadfvffldg dallsnpkti qdlveenrti iapmldsrsa
      181 ysnfwcgmne kgyykrtdey mpilerekvg vfpvvmvhsa tlinlnhads rkltydpqkl
      241 qgytgpnddv itfahsakfa gvemfvsnkd qyghilapte spaqeaeell nlklevtvdg
      301 eplvvsptlh sfvvppekdt lgfdqiparl lwplachkki pdqcyacsyq elllafmlss
      361 yfsavsvtvs llpqrsaapm phvyvlsgtk vtdppskfqt vaparsvprf gt
//
LOCUS       XP_077504631             395 aa            linear   INV 25-NOV-2025
DEFINITION  procollagen galactosyltransferase 1-A-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504631
VERSION     XP_077504631.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648505.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..395
                     /product="procollagen galactosyltransferase 1-A-like
                     isoform X2"
                     /calculated_mol_wt=45051
     Region          7..>165
                     /region_name="BcsA"
                     /note="Glycosyltransferase, catalytic subunit of cellulose
                     synthase and poly-beta-1,6-N-acetylglucosamine synthase
                     [Cell motility]; COG1215"
                     /db_xref="CDD:440828"
     CDS             1..395
                     /gene="LOC144114641"
                     /coded_by="XM_077648505.1:239..1426"
                     /db_xref="GeneID:144114641"
ORIGIN      
        1 mrylsvmffl cfwlpllssv gvvrcatsdd kleaptllia vvlrnkahvl pqffgyleqq
       61 nypksrlslw iytdhnvdnt aeivnswaee vsndyhnvnv tredgdaffp deeglqkwtp
      121 erywhiirlr eeaihvartl wadfvffldg dallsnpkti qdlveenrti iapmldsrsa
      181 ysnfwcgmne kgyykrtdey mpilerekvg vfpvvmvhsa tlinlnhads rkltydpqkl
      241 qgytgpnddv itfahsakfa gvemfvsnkd qyghilapte spaqeaeell nlklevtvdg
      301 eplvvsptlh sfvvppekdt lgfdqlemps fqcqvqnsgl mtcnsegidt egmdsvrhkm
      361 lceryshacg eefmdkhchs syltalstlw fcsfp
//
LOCUS       XP_077504632             400 aa            linear   INV 25-NOV-2025
DEFINITION  procollagen galactosyltransferase 1-A-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504632
VERSION     XP_077504632.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648506.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..400
                     /product="procollagen galactosyltransferase 1-A-like
                     isoform X3"
                     /calculated_mol_wt=45702
     Region          7..>165
                     /region_name="BcsA"
                     /note="Glycosyltransferase, catalytic subunit of cellulose
                     synthase and poly-beta-1,6-N-acetylglucosamine synthase
                     [Cell motility]; COG1215"
                     /db_xref="CDD:440828"
     Region          325..>361
                     /region_name="COG3306"
                     /note="Glycosyltransferase involved in LPS biosynthesis,
                     GR25 family [Cell wall/membrane/envelope biogenesis]"
                     /db_xref="CDD:442535"
     CDS             1..400
                     /gene="LOC144114641"
                     /coded_by="XM_077648506.1:239..1441"
                     /db_xref="GeneID:144114641"
ORIGIN      
        1 mrylsvmffl cfwlpllssv gvvrcatsdd kleaptllia vvlrnkahvl pqffgyleqq
       61 nypksrlslw iytdhnvdnt aeivnswaee vsndyhnvnv tredgdaffp deeglqkwtp
      121 erywhiirlr eeaihvartl wadfvffldg dallsnpkti qdlveenrti iapmldsrsa
      181 ysnfwcgmne kgyykrtdey mpilerekvg vfpvvmvhsa tlinlnhads rkltydpqkl
      241 qgytgpnddv itfahsakfa gvemfvsnkd qyghilapte spaqeaeell nlklevtvdg
      301 eplvvsptlh sfvvppekdt lgfdqvylin lerrperrkr meycfeelgv afkamaavdg
      361 ngglglescl krlvedvgah akeehcrqwr aqlvpiclyt
//
LOCUS       XP_077504633             361 aa            linear   INV 25-NOV-2025
DEFINITION  procollagen galactosyltransferase 1-A-like isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077504633
VERSION     XP_077504633.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648507.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..361
                     /product="procollagen galactosyltransferase 1-A-like
                     isoform X4"
                     /calculated_mol_wt=41354
     Region          7..>165
                     /region_name="BcsA"
                     /note="Glycosyltransferase, catalytic subunit of cellulose
                     synthase and poly-beta-1,6-N-acetylglucosamine synthase
                     [Cell motility]; COG1215"
                     /db_xref="CDD:440828"
     Region          325..>361
                     /region_name="COG3306"
                     /note="Glycosyltransferase involved in LPS biosynthesis,
                     GR25 family [Cell wall/membrane/envelope biogenesis]"
                     /db_xref="CDD:442535"
     CDS             1..361
                     /gene="LOC144114641"
                     /coded_by="XM_077648507.1:236..1321"
                     /db_xref="GeneID:144114641"
ORIGIN      
        1 mrylsvmffl cfwlpllssv gvvrcatsdd kleaptllia vvlrnkahvl pqffgyleqq
       61 nypksrlslw iytdhnvdnt aeivnswaee vsndyhnvnv tredgdaffp deeglqkwtp
      121 erywhiirlr eeaihvartl wadfvffldg dallsnpkti qdlveenrti iapmldsrsa
      181 ysnfwcgmne kgyykrtdey mpilerekvg vfpvvmvhsa tlinlnhads rkltydpqkl
      241 qgytgpnddv itfahsakfa gvemfvsnkd qyghilapte spaqeaeell nlklevtvdg
      301 eplvvsptlh sfvvppekdt lgfdqvylin lerrperrkr meycfeelgv afkamaavdg
      361 k
//
LOCUS       XP_077504634             361 aa            linear   INV 25-NOV-2025
DEFINITION  procollagen galactosyltransferase 1-A-like isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077504634
VERSION     XP_077504634.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648508.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..361
                     /product="procollagen galactosyltransferase 1-A-like
                     isoform X5"
                     /calculated_mol_wt=41354
     Region          7..>165
                     /region_name="BcsA"
                     /note="Glycosyltransferase, catalytic subunit of cellulose
                     synthase and poly-beta-1,6-N-acetylglucosamine synthase
                     [Cell motility]; COG1215"
                     /db_xref="CDD:440828"
     Region          325..>361
                     /region_name="COG3306"
                     /note="Glycosyltransferase involved in LPS biosynthesis,
                     GR25 family [Cell wall/membrane/envelope biogenesis]"
                     /db_xref="CDD:442535"
     CDS             1..361
                     /gene="LOC144114641"
                     /coded_by="XM_077648508.1:236..1321"
                     /db_xref="GeneID:144114641"
ORIGIN      
        1 mrylsvmffl cfwlpllssv gvvrcatsdd kleaptllia vvlrnkahvl pqffgyleqq
       61 nypksrlslw iytdhnvdnt aeivnswaee vsndyhnvnv tredgdaffp deeglqkwtp
      121 erywhiirlr eeaihvartl wadfvffldg dallsnpkti qdlveenrti iapmldsrsa
      181 ysnfwcgmne kgyykrtdey mpilerekvg vfpvvmvhsa tlinlnhads rkltydpqkl
      241 qgytgpnddv itfahsakfa gvemfvsnkd qyghilapte spaqeaeell nlklevtvdg
      301 eplvvsptlh sfvvppekdt lgfdqvylin lerrperrkr meycfeelgv afkamaavdg
      361 k
//
LOCUS       XP_077504635             349 aa            linear   INV 25-NOV-2025
DEFINITION  procollagen galactosyltransferase 1-A-like isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077504635
VERSION     XP_077504635.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648509.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..349
                     /product="procollagen galactosyltransferase 1-A-like
                     isoform X6"
                     /calculated_mol_wt=39724
     Region          7..>165
                     /region_name="BcsA"
                     /note="Glycosyltransferase, catalytic subunit of cellulose
                     synthase and poly-beta-1,6-N-acetylglucosamine synthase
                     [Cell motility]; COG1215"
                     /db_xref="CDD:440828"
     CDS             1..349
                     /gene="LOC144114641"
                     /coded_by="XM_077648509.1:236..1285"
                     /db_xref="GeneID:144114641"
ORIGIN      
        1 mrylsvmffl cfwlpllssv gvvrcatsdd kleaptllia vvlrnkahvl pqffgyleqq
       61 nypksrlslw iytdhnvdnt aeivnswaee vsndyhnvnv tredgdaffp deeglqkwtp
      121 erywhiirlr eeaihvartl wadfvffldg dallsnpkti qdlveenrti iapmldsrsa
      181 ysnfwcgmne kgyykrtdey mpilerekvg vfpvvmvhsa tlinlnhads rkltydpqkl
      241 qgytgpnddv itfahsakfa gvemfvsnkd qyghilapte spaqeaeell nlklevtvdg
      301 eplvvsptlh sfvvppekdt lgfdqchscy lmavyshcsl srssnaiar
//
LOCUS       XP_077504636             312 aa            linear   INV 25-NOV-2025
DEFINITION  sulfotransferase ssu-1-like [Amblyomma americanum].
ACCESSION   XP_077504636
VERSION     XP_077504636.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648510.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..312
                     /product="sulfotransferase ssu-1-like"
                     /calculated_mol_wt=35461
     Region          35..301
                     /region_name="Sulfotransfer_1"
                     /note="Sulfotransferase domain; pfam00685"
                     /db_xref="CDD:425820"
     CDS             1..312
                     /gene="LOC144114642"
                     /coded_by="XM_077648510.1:178..1116"
                     /db_xref="GeneID:144114642"
ORIGIN      
        1 mdadayrevn gvwlhnifhe knirsainyq prsgdlfvia ypkcgsiwtq nivsciltrg
       61 rpasshsdvg lvtrfidlrg aaaadkpskc spmlthlppa vfkptekaky iyvarnpydc
      121 avshyyflkg ftpkttadvs ferflsifla gkvsygnyfd hlvpwyehrg ddnvlfitye
      181 qlkadtkglv lkiadfmgee hgaslrqddq llqrimyecs vqrmrevyqe kpeermkkva
      241 qatseahsen kkqsaeekas dkhegsgfvr kavvgdwknh ftpeqiektk awiaektrgs
      301 dvmnlwigld lp
//
LOCUS       XP_077504637             215 aa            linear   INV 25-NOV-2025
DEFINITION  glycosyltransferase 25 family member-like [Amblyomma americanum].
ACCESSION   XP_077504637
VERSION     XP_077504637.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648511.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..215
                     /product="glycosyltransferase 25 family member-like"
                     /calculated_mol_wt=24437
     Region          <3..163
                     /region_name="COG3306"
                     /note="Glycosyltransferase involved in LPS biosynthesis,
                     GR25 family [Cell wall/membrane/envelope biogenesis]"
                     /db_xref="CDD:442535"
     CDS             1..215
                     /gene="LOC144114643"
                     /coded_by="XM_077648511.1:78..725"
                     /db_xref="GeneID:144114643"
ORIGIN      
        1 mlpeyadpyh nrpitageig cfmshyniwk dmienqhria iifeddirfe pyfrtkvael
       61 laevrhfdwd liylgrkrls gsnepfvags srlvhvdysy wtlcyaltla garklveadp
      121 lprmvpvdey lpimfdkhpe ktwaahfprr dlkafsvhpl fvypthytge ehyisdteds
      181 vvvdttaapd aaagtehgvv lppvssgpss areel
//
LOCUS       XP_077504638             314 aa            linear   INV 25-NOV-2025
DEFINITION  sulfotransferase 1B1-like [Amblyomma americanum].
ACCESSION   XP_077504638
VERSION     XP_077504638.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648512.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..314
                     /product="sulfotransferase 1B1-like"
                     /calculated_mol_wt=36693
     Region          33..303
                     /region_name="Sulfotransfer_1"
                     /note="Sulfotransferase domain; pfam00685"
                     /db_xref="CDD:425820"
     CDS             1..314
                     /gene="LOC144114644"
                     /coded_by="XM_077648512.1:189..1133"
                     /db_xref="GeneID:144114644"
ORIGIN      
        1 mnteefrdvd glwmhsffpe vlirsamtyr prpddvfivt ypkcgttwtq ylvlsilskg
       61 hpptkavdfm laspylemmg seaaetmvrp gvlkthlpft kqpyskqaky iyvarnpydv
      121 cvsyyyhmrs mtpksvtdvs fgkfhemfia gkvsygdyfd hllswyehrq eqnvlfftye
      181 qmkadtnfwt vqiasfldet ygkelrnnpt llrrvvdats fsnmkdvfnd evqtlvkdml
      241 dlpddkliks mvvyrqkvts teemheddgy irkgivgdwk ghftaeqier tkmwiaektk
      301 gsdvmylwkd ldlp
//
LOCUS       XP_077504639             801 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077504639
VERSION     XP_077504639.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648513.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..801
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=88679
     Region          113..235
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          328..740
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..801
                     /gene="LOC144114645"
                     /coded_by="XM_077648513.1:333..2738"
                     /db_xref="GeneID:144114645"
ORIGIN      
        1 mnrrgryclt altlwatlll cctlwaqkcr aesslpaape eirtqdsnge dvasrahsrg
       61 lqlgvdssne teaarrqwkd qegrlkarve sffkmlfpnl vrlgseaeis aecqaayfkm
      121 flairqlktw alqmvdssgk ipngvmagta asfghfdqcl ditvdgdafh gkfcnlelwl
      181 kdlprpspna adviedsesv ltffydngyw lqlfparlgv cipstcsred lqkmaakasq
      241 elqmsanvts cqvkepiafs keqlvvlgil gvvllltvvg taldalckqd vkhtstfggr
      301 lavslasfsl vrntralffr sehaagplea lngirvvscf wivlghiyfl tdlstyirft
      361 sltklqvlfk dflftlvenf tlpvdtfffi tglllaynqt rrclqtnsri siwsmistvf
      421 hrywrttpaf alatalfvlm pafgsgpmwe dvlglasenc rlrwwsnlly fsnylsyskm
      481 cmlhswflsl nmqyfvvglp lalllfkspr vtlvivaalv vasclvtgmv tyindlppam
      541 lmmtarwska kqllnmvyyl pfthfgpfav glcfgyalpt akvprfgkvt ltvgwlvaav
      601 lcgsavfvay pfrrsdpsfh pllsavyagc hrtiwtvgva wvtllcatrq gglvgellss
      661 simtplsrls ylvfllhpvp iyvhvasvre sfqldhyymc tlyfgilvts ilmayvayvt
      721 veapfgalek llqspsesaa griatplpth cpepqqpqqq hradgwrspq rtaaaatsaa
      781 vnggsstgii anaklsessh l
//
LOCUS       XP_077504640             274 aa            linear   INV 25-NOV-2025
DEFINITION  aurora kinase A-B-like [Amblyomma americanum].
ACCESSION   XP_077504640
VERSION     XP_077504640.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648514.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..274
                     /product="aurora kinase A-B-like"
                     /calculated_mol_wt=31738
     Region          10..262
                     /region_name="STKc_Aurora"
                     /note="Catalytic domain of the Serine/Threonine kinase,
                     Aurora kinase; cd14007"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,88..91,95,97,134,136,138..139,
                     141,152,155,169..172)
                     /site_type="active"
                     /db_xref="CDD:270909"
     Site            order(17..21,25,38,40,72,89,91,95,138..139,141,152)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270909"
     Site            order(21,95,97,134,136,138,155,169..172)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270909"
     Site            order(151..160,163..172)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270909"
     CDS             1..274
                     /gene="LOC144114646"
                     /coded_by="XM_077648514.1:188..1012"
                     /db_xref="GeneID:144114646"
ORIGIN      
        1 msvkewclkd feigrplgkg kfgnvylare ketkfivalk vmfksqlqkn nvehqlrrei
       61 eiqshlrhpn ilclynwfhd etriyliley apqgelyrhl tkakrftnqk aatyiyqlcn
      121 alkvchshkv ihrdikpenl llgcngeiki adfgwsvhap ssrretmcgt ldylppemie
      181 gavynekvdh walgiliyef lvgkppfear ttqqtyrric gakvkfprhv sadardiigk
      241 llkrlpadri sldevlthpw vtkhadttve cktl
//
LOCUS       XP_077504641             309 aa            linear   INV 25-NOV-2025
DEFINITION  polyprenal reductase [Amblyomma americanum].
ACCESSION   XP_077504641
VERSION     XP_077504641.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648515.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..309
                     /product="polyprenal reductase"
                     /calculated_mol_wt=35245
     Region          64..308
                     /region_name="ICMT"
                     /note="Isoprenylcysteine carboxyl methyltransferase (ICMT)
                     family; cl21511"
                     /db_xref="CDD:473892"
     CDS             1..309
                     /gene="LOC144114647"
                     /coded_by="XM_077648515.1:350..1279"
                     /db_xref="GeneID:144114647"
ORIGIN      
        1 mvftlldvvn pldvfwtlvt lamvlasccy fvkkdaapgl ltaamlygkv aetnkktrll
       61 kdisipkrwf khfyqfgvvi ftfctlcmvr syifglplps mvskvvsffe pqqstaasat
      121 sillvqvlet cqvyrrcyec tfvsvysnvr mhiwhylmgy ffyfgvqlti lanapglsgf
      181 tvpkfsvldi sahhiigiav flwafyvqfd shlrmaslrk dpkgsvvtln hkiphggmft
      241 yvscphymae lviycalslv lgkpnttwwl mmawnwsnqv aiaflshnwy renfssypkt
      301 rkaiipfvl
//
LOCUS       XP_077504642             456 aa            linear   INV 25-NOV-2025
DEFINITION  kynurenine aminotransferase isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504642
VERSION     XP_077504642.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648516.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..456
                     /product="kynurenine aminotransferase isoform X1"
                     /calculated_mol_wt=50921
     Region          45..452
                     /region_name="AspB"
                     /note="Aspartate/methionine/tyrosine aminotransferase
                     [Amino acid transport and metabolism]; COG0436"
                     /db_xref="CDD:440205"
     CDS             1..456
                     /gene="Kyat"
                     /coded_by="XM_077648516.1:215..1585"
                     /db_xref="GeneID:144114649"
ORIGIN      
        1 mlrtlkfvvt tsssryapll gtkghsinpl ssafysgtqk kmakmevakr vqgmqdsvwv
       61 efiqlaqetk pvnlgqgfpd fpppdfvvka lqeaaagnll hqytrgyghl rlvnalakmy
      121 srltgrqidp qkevlvtvga yealyttilg lvnpgdevii iepffdcyep mtlmaggvpv
      181 fiplrpkkqg qissadwvld pkelaskftp ktkmiivntp hnpigkvfsr eelemiaelc
      241 kkhdvicvmd evyewiiyeg vkhirmntlp gmwertitig sagktfsitg wkvgwaygph
      301 hllkglqlvh qnciytlstl lqeaiavgle kemervsdpe gywnslsssl qkkrdficsf
      361 lssvgmtptv peggyflvcd fsqlaskvkl egnepkdylf akwlsrsrkl qgippsafyg
      421 adhkplaqdl irfcfikdds tlqkaadila elknms
//
LOCUS       XP_077504643             415 aa            linear   INV 25-NOV-2025
DEFINITION  kynurenine aminotransferase isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504643
VERSION     XP_077504643.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648517.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..415
                     /product="kynurenine aminotransferase isoform X2"
                     /calculated_mol_wt=46465
     Region          4..411
                     /region_name="AspB"
                     /note="Aspartate/methionine/tyrosine aminotransferase
                     [Amino acid transport and metabolism]; COG0436"
                     /db_xref="CDD:440205"
     CDS             1..415
                     /gene="Kyat"
                     /coded_by="XM_077648517.1:286..1533"
                     /db_xref="GeneID:144114649"
ORIGIN      
        1 makmevakrv qgmqdsvwve fiqlaqetkp vnlgqgfpdf pppdfvvkal qeaaagnllh
       61 qytrgyghlr lvnalakmys rltgrqidpq kevlvtvgay ealyttilgl vnpgdeviii
      121 epffdcyepm tlmaggvpvf iplrpkkqgq issadwvldp kelaskftpk tkmiivntph
      181 npigkvfsre elemiaelck khdvicvmde vyewiiyegv khirmntlpg mwertitigs
      241 agktfsitgw kvgwaygphh llkglqlvhq nciytlstll qeaiavglek emervsdpeg
      301 ywnslssslq kkrdficsfl ssvgmtptvp eggyflvcdf sqlaskvkle gnepkdylfa
      361 kwlsrsrklq gippsafyga dhkplaqdli rfcfikddst lqkaadilae lknms
//
LOCUS       XP_077504644             415 aa            linear   INV 25-NOV-2025
DEFINITION  kynurenine aminotransferase isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504644
VERSION     XP_077504644.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648518.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..415
                     /product="kynurenine aminotransferase isoform X2"
                     /calculated_mol_wt=46465
     Region          4..411
                     /region_name="AspB"
                     /note="Aspartate/methionine/tyrosine aminotransferase
                     [Amino acid transport and metabolism]; COG0436"
                     /db_xref="CDD:440205"
     CDS             1..415
                     /gene="Kyat"
                     /coded_by="XM_077648518.1:26..1273"
                     /db_xref="GeneID:144114649"
ORIGIN      
        1 makmevakrv qgmqdsvwve fiqlaqetkp vnlgqgfpdf pppdfvvkal qeaaagnllh
       61 qytrgyghlr lvnalakmys rltgrqidpq kevlvtvgay ealyttilgl vnpgdeviii
      121 epffdcyepm tlmaggvpvf iplrpkkqgq issadwvldp kelaskftpk tkmiivntph
      181 npigkvfsre elemiaelck khdvicvmde vyewiiyegv khirmntlpg mwertitigs
      241 agktfsitgw kvgwaygphh llkglqlvhq nciytlstll qeaiavglek emervsdpeg
      301 ywnslssslq kkrdficsfl ssvgmtptvp eggyflvcdf sqlaskvkle gnepkdylfa
      361 kwlsrsrklq gippsafyga dhkplaqdli rfcfikddst lqkaadilae lknms
//
LOCUS       XP_077504645             385 aa            linear   INV 25-NOV-2025
DEFINITION  core histone macro-H2A.1-like [Amblyomma americanum].
ACCESSION   XP_077504645
VERSION     XP_077504645.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648519.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..385
                     /product="core histone macro-H2A.1-like"
                     /calculated_mol_wt=40601
     Region          14..119
                     /region_name="H2A"
                     /note="Histone 2A; smart00414"
                     /db_xref="CDD:197711"
     Region          195..382
                     /region_name="Macro_H2A-like"
                     /note="macrodomain, macroH2A-like family; cd02904"
                     /db_xref="CDD:394875"
     Site            order(218..219,231..233,237,239..242,245,291,326..332,337,
                     368..369,372)
                     /site_type="other"
                     /note="ADP-ribose binding site [chemical binding]"
                     /db_xref="CDD:394875"
     CDS             1..385
                     /gene="LOC144114650"
                     /coded_by="XM_077648519.1:125..1282"
                     /db_xref="GeneID:144114650"
ORIGIN      
        1 msarggkkrc kavsksakag vlfpvgrigr ylrksthhfr igagapvyma avieyltaei
       61 lelagnaard nkkgrvtprh illaiandde lhqllrgvti asggvlpril pellarkkgn
      121 kfimpspkhq ssavnviipp pkakpakpvp ppplkkavta askkqtappp psapalskdk
      181 grsakgkdaa ggelshssvt vlsekklflg qkltviqgdv asvtadaaih ptnanfalsg
      241 evgqvldkvg gkefqqevke llashgples agvaicpghh fpakhvlhch vppasganaa
      301 ellekcvrnc lsladeknir vlaippmvsh stspsqkqqa aqtvlraisn yfvnvmsssl
      361 kqiyfvlsdm esigvytsel aklds
//
LOCUS       XP_077504646             385 aa            linear   INV 25-NOV-2025
DEFINITION  core histone macro-H2A.1-like [Amblyomma americanum].
ACCESSION   XP_077504646
VERSION     XP_077504646.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648520.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..385
                     /product="core histone macro-H2A.1-like"
                     /calculated_mol_wt=40601
     Region          14..119
                     /region_name="H2A"
                     /note="Histone 2A; smart00414"
                     /db_xref="CDD:197711"
     Region          195..382
                     /region_name="Macro_H2A-like"
                     /note="macrodomain, macroH2A-like family; cd02904"
                     /db_xref="CDD:394875"
     Site            order(218..219,231..233,237,239..242,245,291,326..332,337,
                     368..369,372)
                     /site_type="other"
                     /note="ADP-ribose binding site [chemical binding]"
                     /db_xref="CDD:394875"
     CDS             1..385
                     /gene="LOC144114650"
                     /coded_by="XM_077648520.1:125..1282"
                     /db_xref="GeneID:144114650"
ORIGIN      
        1 msarggkkrc kavsksakag vlfpvgrigr ylrksthhfr igagapvyma avieyltaei
       61 lelagnaard nkkgrvtprh illaiandde lhqllrgvti asggvlpril pellarkkgn
      121 kfimpspkhq ssavnviipp pkakpakpvp ppplkkavta askkqtappp psapalskdk
      181 grsakgkdaa ggelshssvt vlsekklflg qkltviqgdv asvtadaaih ptnanfalsg
      241 evgqvldkvg gkefqqevke llashgples agvaicpghh fpakhvlhch vppasganaa
      301 ellekcvrnc lsladeknir vlaippmvsh stspsqkqqa aqtvlraisn yfvnvmsssl
      361 kqiyfvlsdm esigvytsel aklds
//
LOCUS       XP_077504647             385 aa            linear   INV 25-NOV-2025
DEFINITION  core histone macro-H2A.1-like [Amblyomma americanum].
ACCESSION   XP_077504647
VERSION     XP_077504647.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648521.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..385
                     /product="core histone macro-H2A.1-like"
                     /calculated_mol_wt=40601
     Region          14..119
                     /region_name="H2A"
                     /note="Histone 2A; smart00414"
                     /db_xref="CDD:197711"
     Region          195..382
                     /region_name="Macro_H2A-like"
                     /note="macrodomain, macroH2A-like family; cd02904"
                     /db_xref="CDD:394875"
     Site            order(218..219,231..233,237,239..242,245,291,326..332,337,
                     368..369,372)
                     /site_type="other"
                     /note="ADP-ribose binding site [chemical binding]"
                     /db_xref="CDD:394875"
     CDS             1..385
                     /gene="LOC144114650"
                     /coded_by="XM_077648521.1:125..1282"
                     /db_xref="GeneID:144114650"
ORIGIN      
        1 msarggkkrc kavsksakag vlfpvgrigr ylrksthhfr igagapvyma avieyltaei
       61 lelagnaard nkkgrvtprh illaiandde lhqllrgvti asggvlpril pellarkkgn
      121 kfimpspkhq ssavnviipp pkakpakpvp ppplkkavta askkqtappp psapalskdk
      181 grsakgkdaa ggelshssvt vlsekklflg qkltviqgdv asvtadaaih ptnanfalsg
      241 evgqvldkvg gkefqqevke llashgples agvaicpghh fpakhvlhch vppasganaa
      301 ellekcvrnc lsladeknir vlaippmvsh stspsqkqqa aqtvlraisn yfvnvmsssl
      361 kqiyfvlsdm esigvytsel aklds
//
LOCUS       XP_077504648             385 aa            linear   INV 25-NOV-2025
DEFINITION  core histone macro-H2A.1-like [Amblyomma americanum].
ACCESSION   XP_077504648
VERSION     XP_077504648.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648522.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..385
                     /product="core histone macro-H2A.1-like"
                     /calculated_mol_wt=40601
     Region          14..119
                     /region_name="H2A"
                     /note="Histone 2A; smart00414"
                     /db_xref="CDD:197711"
     Region          195..382
                     /region_name="Macro_H2A-like"
                     /note="macrodomain, macroH2A-like family; cd02904"
                     /db_xref="CDD:394875"
     Site            order(218..219,231..233,237,239..242,245,291,326..332,337,
                     368..369,372)
                     /site_type="other"
                     /note="ADP-ribose binding site [chemical binding]"
                     /db_xref="CDD:394875"
     CDS             1..385
                     /gene="LOC144114650"
                     /coded_by="XM_077648522.1:125..1282"
                     /db_xref="GeneID:144114650"
ORIGIN      
        1 msarggkkrc kavsksakag vlfpvgrigr ylrksthhfr igagapvyma avieyltaei
       61 lelagnaard nkkgrvtprh illaiandde lhqllrgvti asggvlpril pellarkkgn
      121 kfimpspkhq ssavnviipp pkakpakpvp ppplkkavta askkqtappp psapalskdk
      181 grsakgkdaa ggelshssvt vlsekklflg qkltviqgdv asvtadaaih ptnanfalsg
      241 evgqvldkvg gkefqqevke llashgples agvaicpghh fpakhvlhch vppasganaa
      301 ellekcvrnc lsladeknir vlaippmvsh stspsqkqqa aqtvlraisn yfvnvmsssl
      361 kqiyfvlsdm esigvytsel aklds
//
LOCUS       XP_077504649             385 aa            linear   INV 25-NOV-2025
DEFINITION  core histone macro-H2A.1-like [Amblyomma americanum].
ACCESSION   XP_077504649
VERSION     XP_077504649.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648523.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..385
                     /product="core histone macro-H2A.1-like"
                     /calculated_mol_wt=40601
     Region          14..119
                     /region_name="H2A"
                     /note="Histone 2A; smart00414"
                     /db_xref="CDD:197711"
     Region          195..382
                     /region_name="Macro_H2A-like"
                     /note="macrodomain, macroH2A-like family; cd02904"
                     /db_xref="CDD:394875"
     Site            order(218..219,231..233,237,239..242,245,291,326..332,337,
                     368..369,372)
                     /site_type="other"
                     /note="ADP-ribose binding site [chemical binding]"
                     /db_xref="CDD:394875"
     CDS             1..385
                     /gene="LOC144114650"
                     /coded_by="XM_077648523.1:125..1282"
                     /db_xref="GeneID:144114650"
ORIGIN      
        1 msarggkkrc kavsksakag vlfpvgrigr ylrksthhfr igagapvyma avieyltaei
       61 lelagnaard nkkgrvtprh illaiandde lhqllrgvti asggvlpril pellarkkgn
      121 kfimpspkhq ssavnviipp pkakpakpvp ppplkkavta askkqtappp psapalskdk
      181 grsakgkdaa ggelshssvt vlsekklflg qkltviqgdv asvtadaaih ptnanfalsg
      241 evgqvldkvg gkefqqevke llashgples agvaicpghh fpakhvlhch vppasganaa
      301 ellekcvrnc lsladeknir vlaippmvsh stspsqkqqa aqtvlraisn yfvnvmsssl
      361 kqiyfvlsdm esigvytsel aklds
//
LOCUS       XP_077504650             385 aa            linear   INV 25-NOV-2025
DEFINITION  core histone macro-H2A.1-like [Amblyomma americanum].
ACCESSION   XP_077504650
VERSION     XP_077504650.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648524.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..385
                     /product="core histone macro-H2A.1-like"
                     /calculated_mol_wt=40601
     Region          14..119
                     /region_name="H2A"
                     /note="Histone 2A; smart00414"
                     /db_xref="CDD:197711"
     Region          195..382
                     /region_name="Macro_H2A-like"
                     /note="macrodomain, macroH2A-like family; cd02904"
                     /db_xref="CDD:394875"
     Site            order(218..219,231..233,237,239..242,245,291,326..332,337,
                     368..369,372)
                     /site_type="other"
                     /note="ADP-ribose binding site [chemical binding]"
                     /db_xref="CDD:394875"
     CDS             1..385
                     /gene="LOC144114650"
                     /coded_by="XM_077648524.1:125..1282"
                     /db_xref="GeneID:144114650"
ORIGIN      
        1 msarggkkrc kavsksakag vlfpvgrigr ylrksthhfr igagapvyma avieyltaei
       61 lelagnaard nkkgrvtprh illaiandde lhqllrgvti asggvlpril pellarkkgn
      121 kfimpspkhq ssavnviipp pkakpakpvp ppplkkavta askkqtappp psapalskdk
      181 grsakgkdaa ggelshssvt vlsekklflg qkltviqgdv asvtadaaih ptnanfalsg
      241 evgqvldkvg gkefqqevke llashgples agvaicpghh fpakhvlhch vppasganaa
      301 ellekcvrnc lsladeknir vlaippmvsh stspsqkqqa aqtvlraisn yfvnvmsssl
      361 kqiyfvlsdm esigvytsel aklds
//
LOCUS       XP_077504651             385 aa            linear   INV 25-NOV-2025
DEFINITION  core histone macro-H2A.1-like [Amblyomma americanum].
ACCESSION   XP_077504651
VERSION     XP_077504651.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648525.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..385
                     /product="core histone macro-H2A.1-like"
                     /calculated_mol_wt=40601
     Region          14..119
                     /region_name="H2A"
                     /note="Histone 2A; smart00414"
                     /db_xref="CDD:197711"
     Region          195..382
                     /region_name="Macro_H2A-like"
                     /note="macrodomain, macroH2A-like family; cd02904"
                     /db_xref="CDD:394875"
     Site            order(218..219,231..233,237,239..242,245,291,326..332,337,
                     368..369,372)
                     /site_type="other"
                     /note="ADP-ribose binding site [chemical binding]"
                     /db_xref="CDD:394875"
     CDS             1..385
                     /gene="LOC144114650"
                     /coded_by="XM_077648525.1:125..1282"
                     /db_xref="GeneID:144114650"
ORIGIN      
        1 msarggkkrc kavsksakag vlfpvgrigr ylrksthhfr igagapvyma avieyltaei
       61 lelagnaard nkkgrvtprh illaiandde lhqllrgvti asggvlpril pellarkkgn
      121 kfimpspkhq ssavnviipp pkakpakpvp ppplkkavta askkqtappp psapalskdk
      181 grsakgkdaa ggelshssvt vlsekklflg qkltviqgdv asvtadaaih ptnanfalsg
      241 evgqvldkvg gkefqqevke llashgples agvaicpghh fpakhvlhch vppasganaa
      301 ellekcvrnc lsladeknir vlaippmvsh stspsqkqqa aqtvlraisn yfvnvmsssl
      361 kqiyfvlsdm esigvytsel aklds
//
LOCUS       XP_077504652             385 aa            linear   INV 25-NOV-2025
DEFINITION  core histone macro-H2A.1-like [Amblyomma americanum].
ACCESSION   XP_077504652
VERSION     XP_077504652.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648526.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..385
                     /product="core histone macro-H2A.1-like"
                     /calculated_mol_wt=40601
     Region          14..119
                     /region_name="H2A"
                     /note="Histone 2A; smart00414"
                     /db_xref="CDD:197711"
     Region          195..382
                     /region_name="Macro_H2A-like"
                     /note="macrodomain, macroH2A-like family; cd02904"
                     /db_xref="CDD:394875"
     Site            order(218..219,231..233,237,239..242,245,291,326..332,337,
                     368..369,372)
                     /site_type="other"
                     /note="ADP-ribose binding site [chemical binding]"
                     /db_xref="CDD:394875"
     CDS             1..385
                     /gene="LOC144114650"
                     /coded_by="XM_077648526.1:125..1282"
                     /db_xref="GeneID:144114650"
ORIGIN      
        1 msarggkkrc kavsksakag vlfpvgrigr ylrksthhfr igagapvyma avieyltaei
       61 lelagnaard nkkgrvtprh illaiandde lhqllrgvti asggvlpril pellarkkgn
      121 kfimpspkhq ssavnviipp pkakpakpvp ppplkkavta askkqtappp psapalskdk
      181 grsakgkdaa ggelshssvt vlsekklflg qkltviqgdv asvtadaaih ptnanfalsg
      241 evgqvldkvg gkefqqevke llashgples agvaicpghh fpakhvlhch vppasganaa
      301 ellekcvrnc lsladeknir vlaippmvsh stspsqkqqa aqtvlraisn yfvnvmsssl
      361 kqiyfvlsdm esigvytsel aklds
//
LOCUS       XP_077504653             251 aa            linear   INV 25-NOV-2025
DEFINITION  group XIIA secretory phospholipase A2-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504653
VERSION     XP_077504653.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648527.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..251
                     /product="group XIIA secretory phospholipase A2-like
                     isoform X1"
                     /calculated_mol_wt=28243
     Region          85..251
                     /region_name="Phospholip_A2_3"
                     /note="Prokaryotic phospholipase A2; cl29745"
                     /db_xref="CDD:453050"
     CDS             1..251
                     /gene="LOC144114651"
                     /coded_by="XM_077648527.1:17..772"
                     /db_xref="GeneID:144114651"
ORIGIN      
        1 mldsswttat pqrllqlcwr yalprscvrw dlwdvccplk caarfqllcv tdatmifpsf
       61 hgwyrlipva vtiiawlvsg qtifdyarhl thqaeryldd lkfgikairn glntvegvlk
      121 dskepectyk csdgsspkpk igykadsngc gaygfqleak elpfkemnsc chkhdlcygt
      181 clsdkddcda rflscldsvc ekkasrshke knsqgckaaa hafytgshyl gckaykdaqk
      241 qacycpkkhe l
//
LOCUS       XP_077504654             225 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114651 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504654
VERSION     XP_077504654.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648528.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..225
                     /product="uncharacterized protein LOC144114651 isoform X2"
                     /calculated_mol_wt=25367
     Region          85..>200
                     /region_name="Phospholip_A2_3"
                     /note="Prokaryotic phospholipase A2; cl29745"
                     /db_xref="CDD:453050"
     CDS             1..225
                     /gene="LOC144114651"
                     /coded_by="XM_077648528.1:23..700"
                     /db_xref="GeneID:144114651"
ORIGIN      
        1 mldsswttat pqrllqlcwr yalprscvrw dlwdvccplk caarfqllcv tdatmifpsf
       61 hgwyrlipva vtiiawlvsg qtifdyarhl thqaeryldd lkfgikairn glntvegvlk
      121 dskepectyk csdgsspkpk igykadsngc gaygfqleak elpfkemnsc chkhdlcygt
      181 clsdkddcda rflscldsvc ekkasrshke knsqeicsck aeccq
//
LOCUS       XP_077504655             122 aa            linear   INV 25-NOV-2025
DEFINITION  group XIIA secretory phospholipase A2-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504655
VERSION     XP_077504655.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648529.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..122
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..122
                     /product="group XIIA secretory phospholipase A2-like
                     isoform X3"
                     /calculated_mol_wt=13329
     Region          <5..122
                     /region_name="Phospholip_A2_3"
                     /note="Prokaryotic phospholipase A2; cl29745"
                     /db_xref="CDD:453050"
     CDS             1..122
                     /gene="LOC144114651"
                     /coded_by="XM_077648529.1:164..532"
                     /db_xref="GeneID:144114651"
ORIGIN      
        1 mvtngsspkp kigykadsng cgaygfqlea kelpfkemns cchkhdlcyg tclsdkddcd
       61 arflscldsv cekkasrshk eknsqgckaa ahafytgshy lgckaykdaq kqacycpkkh
      121 el
//
LOCUS       XP_077504656             634 aa            linear   INV 25-NOV-2025
DEFINITION  exonuclease mut-7 homolog isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504656
VERSION     XP_077504656.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648530.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..634
                     /product="exonuclease mut-7 homolog isoform X1"
                     /calculated_mol_wt=73521
     Region          404..592
                     /region_name="DnaQ_like_exo"
                     /note="DnaQ-like (or DEDD) 3'-5' exonuclease domain
                     superfamily; cl10012"
                     /db_xref="CDD:447876"
     Site            order(429..432,489..490,492..494,579)
                     /site_type="active"
                     /db_xref="CDD:176655"
     Site            order(429,431,494,579)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:176655"
     Site            order(430..432,489..490,492..493,579)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:176655"
     CDS             1..634
                     /gene="Nbr"
                     /coded_by="XM_077648530.1:257..2161"
                     /db_xref="GeneID:144114653"
ORIGIN      
        1 mpvtmddgiw gleledvwis akrkkseelv kllhsffgks pncfkcaftl mqkaydfryc
       61 rpdtlaftvm nefqewlssh pdvytllgke vkrhalevvl kqadkalikl vchvyklqdn
      121 pleyvdiiqd llnkhlyela aivvmnlklh gnysieliav plylfdhmql leeflegepe
      181 qqrdivkyld alhnkpsieh lkkhvniklv ckerlyekai akaaskllkq ynlplelcpn
      241 ihysrsrnal rylihkrnae pdypeatwre micfcamflq hayssqvlkq vcckmacvap
      301 lmmchisivs fsqaasshea issfprlrrd llydlmtlqn ydtalsfalk lgyadrwwpr
      361 dllhhrstce pekleelrra wdftndvpdy sgkflqlsln lqdvsmvdsp sglkecirvi
      421 tnynivgida ewkptigltp srlalvqlav wdcvfmldmp klmveladsd wdklysdvls
      481 thrilklgyg iaedlrllae svkrpnakvn rvvdlhnftq klqreyprvi rpvyekrnfk
      541 glaeltysll glplnkseqc sdwekrplrp sqtlyaalda ycllqiyevl vkrgeaenvd
      601 irallevgqq smdgsqpqgy kprpyrrygr krts
//
LOCUS       XP_077504657             634 aa            linear   INV 25-NOV-2025
DEFINITION  exonuclease mut-7 homolog isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504657
VERSION     XP_077504657.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648531.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..634
                     /product="exonuclease mut-7 homolog isoform X1"
                     /calculated_mol_wt=73521
     Region          404..592
                     /region_name="DnaQ_like_exo"
                     /note="DnaQ-like (or DEDD) 3'-5' exonuclease domain
                     superfamily; cl10012"
                     /db_xref="CDD:447876"
     Site            order(429..432,489..490,492..494,579)
                     /site_type="active"
                     /db_xref="CDD:176655"
     Site            order(429,431,494,579)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:176655"
     Site            order(430..432,489..490,492..493,579)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:176655"
     CDS             1..634
                     /gene="Nbr"
                     /coded_by="XM_077648531.1:260..2164"
                     /db_xref="GeneID:144114653"
ORIGIN      
        1 mpvtmddgiw gleledvwis akrkkseelv kllhsffgks pncfkcaftl mqkaydfryc
       61 rpdtlaftvm nefqewlssh pdvytllgke vkrhalevvl kqadkalikl vchvyklqdn
      121 pleyvdiiqd llnkhlyela aivvmnlklh gnysieliav plylfdhmql leeflegepe
      181 qqrdivkyld alhnkpsieh lkkhvniklv ckerlyekai akaaskllkq ynlplelcpn
      241 ihysrsrnal rylihkrnae pdypeatwre micfcamflq hayssqvlkq vcckmacvap
      301 lmmchisivs fsqaasshea issfprlrrd llydlmtlqn ydtalsfalk lgyadrwwpr
      361 dllhhrstce pekleelrra wdftndvpdy sgkflqlsln lqdvsmvdsp sglkecirvi
      421 tnynivgida ewkptigltp srlalvqlav wdcvfmldmp klmveladsd wdklysdvls
      481 thrilklgyg iaedlrllae svkrpnakvn rvvdlhnftq klqreyprvi rpvyekrnfk
      541 glaeltysll glplnkseqc sdwekrplrp sqtlyaalda ycllqiyevl vkrgeaenvd
      601 irallevgqq smdgsqpqgy kprpyrrygr krts
//
LOCUS       XP_077504658             589 aa            linear   INV 25-NOV-2025
DEFINITION  exonuclease mut-7 homolog isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504658
VERSION     XP_077504658.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648532.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..589
                     /product="exonuclease mut-7 homolog isoform X2"
                     /calculated_mol_wt=68600
     Region          359..547
                     /region_name="DnaQ_like_exo"
                     /note="DnaQ-like (or DEDD) 3'-5' exonuclease domain
                     superfamily; cl10012"
                     /db_xref="CDD:447876"
     Site            order(384..387,444..445,447..449,534)
                     /site_type="active"
                     /db_xref="CDD:176655"
     Site            order(384,386,449,534)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:176655"
     Site            order(385..387,444..445,447..448,534)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:176655"
     CDS             1..589
                     /gene="Nbr"
                     /coded_by="XM_077648532.1:259..2028"
                     /db_xref="GeneID:144114653"
ORIGIN      
        1 mpvtmddgiw gleledvwis akrkkseelv kllhsffgks pncfkcaftl mqkaydfryc
       61 rpdtlaftvm nefqewlssh pdvytllgke vkrhalevvl kqadkalikl vchvyklqdn
      121 pleyvdiiqd llnkhlyela aivvmnlklh gnysieliav plylfdhmql leeflegepe
      181 qqrdivkyld alhnkpsieh lkkhvniklv ckerlyekai akaaskllkq ynlplelcpn
      241 ihysrsrnal rylihkrnae pdypeatwre miceaissfp rlrrdllydl mtlqnydtal
      301 sfalklgyad rwwprdllhh rstcepekle elrrawdftn dvpdysgkfl qlslnlqdvs
      361 mvdspsglke cirvitnyni vgidaewkpt igltpsrlal vqlavwdcvf mldmpklmve
      421 ladsdwdkly sdvlsthril klgygiaedl rllaesvkrp nakvnrvvdl hnftqklqre
      481 yprvirpvye krnfkglael tysllglpln kseqcsdwek rplrpsqtly aaldaycllq
      541 iyevlvkrge aenvdirall evgqqsmdgs qpqgykprpy rrygrkrts
//
LOCUS       XP_077504659             589 aa            linear   INV 25-NOV-2025
DEFINITION  exonuclease mut-7 homolog isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504659
VERSION     XP_077504659.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648533.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..589
                     /product="exonuclease mut-7 homolog isoform X2"
                     /calculated_mol_wt=68600
     Region          359..547
                     /region_name="DnaQ_like_exo"
                     /note="DnaQ-like (or DEDD) 3'-5' exonuclease domain
                     superfamily; cl10012"
                     /db_xref="CDD:447876"
     Site            order(384..387,444..445,447..449,534)
                     /site_type="active"
                     /db_xref="CDD:176655"
     Site            order(384,386,449,534)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:176655"
     Site            order(385..387,444..445,447..448,534)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:176655"
     CDS             1..589
                     /gene="Nbr"
                     /coded_by="XM_077648533.1:303..2072"
                     /db_xref="GeneID:144114653"
ORIGIN      
        1 mpvtmddgiw gleledvwis akrkkseelv kllhsffgks pncfkcaftl mqkaydfryc
       61 rpdtlaftvm nefqewlssh pdvytllgke vkrhalevvl kqadkalikl vchvyklqdn
      121 pleyvdiiqd llnkhlyela aivvmnlklh gnysieliav plylfdhmql leeflegepe
      181 qqrdivkyld alhnkpsieh lkkhvniklv ckerlyekai akaaskllkq ynlplelcpn
      241 ihysrsrnal rylihkrnae pdypeatwre miceaissfp rlrrdllydl mtlqnydtal
      301 sfalklgyad rwwprdllhh rstcepekle elrrawdftn dvpdysgkfl qlslnlqdvs
      361 mvdspsglke cirvitnyni vgidaewkpt igltpsrlal vqlavwdcvf mldmpklmve
      421 ladsdwdkly sdvlsthril klgygiaedl rllaesvkrp nakvnrvvdl hnftqklqre
      481 yprvirpvye krnfkglael tysllglpln kseqcsdwek rplrpsqtly aaldaycllq
      541 iyevlvkrge aenvdirall evgqqsmdgs qpqgykprpy rrygrkrts
//
LOCUS       XP_077504660             701 aa            linear   INV 25-NOV-2025
DEFINITION  trynity isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504660
VERSION     XP_077504660.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648534.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..701
                     /product="trynity isoform X1"
                     /calculated_mol_wt=77944
     Region          118..195
                     /region_name="PAN_AP_HGF"
                     /note="Subfamily of PAN/APPLE-like domains; present in
                     N-terminal (N) domains of plasminogen/hepatocyte growth
                     factor proteins, and various proteins found in Bilateria,
                     such as leech anti-platelet proteins. PAN/APPLE domains
                     fulfill diverse biological functions...; cd01099"
                     /db_xref="CDD:238532"
     Site            order(133,135,147)
                     /site_type="active"
                     /note="putative binding site [active]"
                     /db_xref="CDD:238532"
     Region          228..>261
                     /region_name="PAN_APPLE"
                     /note="PAN/APPLE-like domain; present in N-terminal (N)
                     domains of plasminogen/ hepatocyte growth factor proteins,
                     plasma prekallikrein/coagulation factor XI and microneme
                     antigen proteins, plant receptor-like protein kinases, and
                     various nematode and leech...; cl00112"
                     /db_xref="CDD:469620"
     Site            order(233,235,247)
                     /site_type="active"
                     /note="putative binding site [active]"
                     /db_xref="CDD:238514"
     Region          364..585
                     /region_name="ZP"
                     /note="Zona pellucida (ZP) domain; smart00241"
                     /db_xref="CDD:214579"
     CDS             1..701
                     /gene="tyn"
                     /coded_by="XM_077648534.1:267..2372"
                     /db_xref="GeneID:144114655"
ORIGIN      
        1 mgrrhcvtll vlahvttlvs gkvsfekltn ldfagtayyt irnlslyecq gwcrdekeca
       61 aaafsfvlnp lapqqettcl lqnstqarkp tarpqravnl yylvknhirs ekicdrlwsf
      121 erfpnkmvrg hdtavlftas kdsclaacln edrfvcrsae fnyvtlqchl sdvdrryagv
      181 qekygdaigv dyfenaclea savcsgmrty dfaqvgvsqd vvthyvdlnf ypdkellvns
      241 kseclractv dndfvcrsvl ykpsqdgtlq gscsvfhvdh lmlpdgpatf agstpplpll
      301 dsgetkgiyl esrctnstrq ytakpgtlks ppsetrtstk spvfadspdp scdaygvcyd
      361 vsiqctdaki vvyvktsrpf hgriyamgrs etcntsvrns qafrldlslt gqdcntqsmg
      421 gvytntvvlq hhnvvltkad kvynvrctye tssknisfgm mpvsdldfrd pdttqitasp
      481 eaplpkivif gvdgreastv rigdrltfri eipettpygi farsciamak darstfeiid
      541 ergcpvdpti fpgfiqiess lqssyeafrf tesygvifqc nvkycvgrce pvvcthgren
      601 ieswgrrkrs arsgkrtsee mtlsqeilvl digdepiars mterpptvne slsyqetvel
      661 mdkcasknsv malsitastl lviyictvay fvaqrkvksi t
//
LOCUS       XP_077504661             696 aa            linear   INV 25-NOV-2025
DEFINITION  trynity isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504661
VERSION     XP_077504661.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648535.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..696
                     /product="trynity isoform X2"
                     /calculated_mol_wt=77366
     Region          118..195
                     /region_name="PAN_AP_HGF"
                     /note="Subfamily of PAN/APPLE-like domains; present in
                     N-terminal (N) domains of plasminogen/hepatocyte growth
                     factor proteins, and various proteins found in Bilateria,
                     such as leech anti-platelet proteins. PAN/APPLE domains
                     fulfill diverse biological functions...; cd01099"
                     /db_xref="CDD:238532"
     Site            order(133,135,147)
                     /site_type="active"
                     /note="putative binding site [active]"
                     /db_xref="CDD:238532"
     Region          228..>261
                     /region_name="PAN_APPLE"
                     /note="PAN/APPLE-like domain; present in N-terminal (N)
                     domains of plasminogen/ hepatocyte growth factor proteins,
                     plasma prekallikrein/coagulation factor XI and microneme
                     antigen proteins, plant receptor-like protein kinases, and
                     various nematode and leech...; cl00112"
                     /db_xref="CDD:469620"
     Site            order(233,235,247)
                     /site_type="active"
                     /note="putative binding site [active]"
                     /db_xref="CDD:238514"
     Region          364..580
                     /region_name="ZP"
                     /note="Zona pellucida (ZP) domain; smart00241"
                     /db_xref="CDD:214579"
     CDS             1..696
                     /gene="tyn"
                     /coded_by="XM_077648535.1:174..2264"
                     /db_xref="GeneID:144114655"
ORIGIN      
        1 mgrrhcvtll vlahvttlvs gkvsfekltn ldfagtayyt irnlslyecq gwcrdekeca
       61 aaafsfvlnp lapqqettcl lqnstqarkp tarpqravnl yylvknhirs ekicdrlwsf
      121 erfpnkmvrg hdtavlftas kdsclaacln edrfvcrsae fnyvtlqchl sdvdrryagv
      181 qekygdaigv dyfenaclea savcsgmrty dfaqvgvsqd vvthyvdlnf ypdkellvns
      241 kseclractv dndfvcrsvl ykpsqdgtlq gscsvfhvdh lmlpdgpatf agstpplpll
      301 dsgetkgiyl esrctnstrq ytakpgtlks ppsetrtstk spvfadspdp scdaygvcyd
      361 vsiqctdaki vvyvktsrpf hgriyamgrs etcntsvrns qafrldlslt gqdcntqsmg
      421 gvytntvvlq hhnvvltkad kvynvrctye tssknisfgm mpvrdpdttq itaspeaplp
      481 kivifgvdgr eastvrigdr ltfrieipet tpygifarsc iamakdarst feiidergcp
      541 vdptifpgfi qiesslqssy eafrftesyg vifqcnvkyc vgrcepvvct hgrenieswg
      601 rrkrsarsgk rtseemtlsq eilvldigde piarsmterp ptvneslsyq etvelmdkca
      661 sknsvmalsi tastllviyi ctvayfvaqr kvksit
//
LOCUS       XP_077504662             603 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114656 [Amblyomma americanum].
ACCESSION   XP_077504662
VERSION     XP_077504662.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648536.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..603
                     /product="uncharacterized protein LOC144114656"
                     /calculated_mol_wt=67153
     Region          12..42
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          <159..186
                     /region_name="UBA_like_SF"
                     /note="UBA domain-like superfamily; cl21463"
                     /db_xref="CDD:473871"
     Region          268..304
                     /region_name="CUE"
                     /note="CUE domain found in ubiquitin-binding CUE proteins;
                     cd14279"
                     /db_xref="CDD:270465"
     CDS             1..603
                     /gene="LOC144114656"
                     /coded_by="XM_077648536.1:79..1890"
                     /db_xref="GeneID:144114656"
ORIGIN      
        1 maavhpepae tlpsgwecrf dprtgrnyfi nhrehtttwe dprlrypqlq qgsyakyyly
       61 gsgkptpgqa atkqkyfqdd lamacppyyg mvyyppppps ttvsnsatas pregfytaaa
      121 rpvgtyspyf gsprtpyrfk sdtavpvdeq llmklclqfp tveeghiftl lkqyhnrenv
      181 vvsallaegh praqthpnah ehlppkllkv deasvsklhg ffptidqeti rgllykhnnq
      241 ehevirmlvc gpnnyiirhq spapsspkmk lrylkllfpe adeamlfnml yncdnnaqea
      301 mtrlndlgyk krdaplprgt pvkatpaklp tvevrrpsqv vpptkadrqk fvsrlheqfp
      361 avsqtllnma lessqfneer akqflramtp qdsrpllgse giqpgvlvks tppvpprsrh
      421 aspaqtdglt latksapstk egsskeetqf itvsdkatsp ppksiteraf rpkflrklfk
      481 fskgtsthed qmtqpssric pkgpnasllk gpndgnllre yvqwqgpdpd nrrgpdpslr
      541 rgpdpknlts kegctkargp qasslfrrgp lkditfrsfl rgssmssggs dspqgsksss
      601 sqq
//
LOCUS       XP_077504663             124 aa            linear   INV 25-NOV-2025
DEFINITION  tRNA methyltransferase subunit 11-2 [Amblyomma americanum].
ACCESSION   XP_077504663
VERSION     XP_077504663.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648537.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..124
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..124
                     /product="tRNA methyltransferase subunit 11-2"
                     /calculated_mol_wt=13727
     Region          2..119
                     /region_name="Trm112-like"
                     /note="eukaryotic tRNA methyltransferase 112, a partner
                     protein of both rRNA/tRNA and protein methyltransferases,
                     and similar proteins; cd21089"
                     /db_xref="CDD:411041"
     Site            order(2,5,7..10,12,21,38,41,44,48,50,112,114..118)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:411041"
     CDS             1..124
                     /gene="Trmt112"
                     /coded_by="XM_077648537.1:430..804"
                     /db_xref="GeneID:144114657"
ORIGIN      
        1 mkllthnmmt skcikgvntg fplgivaset kvisvdfnpe fvcrmipkld wdalyqaaes
       61 igcgshlpkt lvpdyehnee flkqvhhtlf evevvagelv cpetgrkfpi tngipnmlln
      121 edev
//
LOCUS       XP_077504664            1030 aa            linear   INV 25-NOV-2025
DEFINITION  lysosomal alpha-mannosidase-like [Amblyomma americanum].
ACCESSION   XP_077504664
VERSION     XP_077504664.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648538.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1030
                     /product="lysosomal alpha-mannosidase-like"
                     /calculated_mol_wt=117609
     Region          60..822
                     /region_name="PLN02701"
                     /note="alpha-mannosidase"
                     /db_xref="CDD:178304"
     Region          60..323
                     /region_name="GH38N_AMII_LAM_like"
                     /note="N-terminal catalytic domain of lysosomal
                     alpha-mannosidase and similar proteins; glycoside
                     hydrolase family 38 (GH38); cd10810"
                     /db_xref="CDD:212121"
     Site            order(69,71,74,185,187,209,301)
                     /site_type="active"
                     /db_xref="CDD:212121"
     Site            order(71..77,144..160,162..163,185,187..192,196,209..214,
                     230..232)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:212121"
     CDS             1..1030
                     /gene="LOC144114660"
                     /coded_by="XM_077648538.1:93..3185"
                     /db_xref="GeneID:144114660"
ORIGIN      
        1 mrrdpvvqgl atmlllsgml slvlcsalag lqhrqaaqre rrhetaciyk achepkpgfl
       61 nvhivshthn dvgwvhtvnf yyrnyvrtil dsvveelean rdrrfiyvem afftrwweeq
      121 tpekqasvra lvedgrlefa gggwcmndea tthytatvde mslglrwlna tfgqcgrarv
      181 awqidpfgha rqeaalfaqm gfdglflgri hlddkdwrqh nkqmeflwka dkyigekgdi
      241 ftgvlpnvyw pptgfcfdaw cvdetldsyn grrrareflq ivtkqakyya tnhtvvtmgm
      301 dfhyrdaskw frnldsliyy inaeqangsr vhafystpac ylkalhdsgv rwpefsddff
      361 pyadqehayw tgyytsrpnf kyfarqangi lqackqlkvi gqlddsadvd tlaravavlq
      421 hhdaisgtqk qhvaedyvym tdmgirqced vfnkayrrml lsnsggtafq qdicltfcpl
      481 lnisscdite tadeffvfvy nplstpvrty arlpisgdgy evkelpgqvl eaqlvpvqrk
      541 vlriperkat aatteivfpm hlpplgvtty qlrkyegftl rdsessavfd ggseapaidn
      601 gkyrlfvdpv tgllsrvmll ntglevpfrq slfaymayeg vvekpsgays fnpaddqpvd
      661 lgrrvnytlv kgplvqeihq tfndwasqvi rlykdedlie fdwvigpipf segrepsrgk
      721 eiisrfqtsi wnngtfytds ngrqnikrtr naqrpwtkti vepttynyyp vvswiylrne
      781 shnlqltvfp drpqggsslh egelelmlhr rlqyddsfgv eeplneigvd krglvargth
      841 rvflgsveds ermlrtmanr mvfgpqfafh trdrqlldip vprasglqyp lpanlhlltl
      901 ehignsraiv rlehlypgvk dnrlnrpasl slkhllvsyt vtaveeavls aheslrnstr
      961 fhfetrdsvs ssspellryl aaggrrnngr gsaslsrrtp spiderlftv tvnpgeirtf
     1021 lvtlepkrid
//
LOCUS       XP_077504665             262 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114661 [Amblyomma americanum].
ACCESSION   XP_077504665
VERSION     XP_077504665.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648539.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..262
                     /product="uncharacterized protein LOC144114661"
                     /calculated_mol_wt=28219
     CDS             1..262
                     /gene="LOC144114661"
                     /coded_by="XM_077648539.1:961..1749"
                     /db_xref="GeneID:144114661"
ORIGIN      
        1 magsndclhg ravtfsleri lrtgslcpsq asnvengqtv lsmhgaslnv tsseldvpae
       61 ataqaathds aepaqvapar fvdtgdfvmh iyelftslka mplqrppsti anssiayiag
      121 ivaraveerr tcsfcpplhq sngpspvlmg lidlqsrggl tfpkskfvtv lvtvkkavdi
      181 alphikksnm rqklaelllp rleqcplfvc parddhaast lsvvftkfmr pllanvgatv
      241 tdraayrkkl ackplhrkvh rv
//
LOCUS       XP_077504666             262 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114661 [Amblyomma americanum].
ACCESSION   XP_077504666
VERSION     XP_077504666.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648540.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..262
                     /product="uncharacterized protein LOC144114661"
                     /calculated_mol_wt=28219
     CDS             1..262
                     /gene="LOC144114661"
                     /coded_by="XM_077648540.1:487..1275"
                     /db_xref="GeneID:144114661"
ORIGIN      
        1 magsndclhg ravtfsleri lrtgslcpsq asnvengqtv lsmhgaslnv tsseldvpae
       61 ataqaathds aepaqvapar fvdtgdfvmh iyelftslka mplqrppsti anssiayiag
      121 ivaraveerr tcsfcpplhq sngpspvlmg lidlqsrggl tfpkskfvtv lvtvkkavdi
      181 alphikksnm rqklaelllp rleqcplfvc parddhaast lsvvftkfmr pllanvgatv
      241 tdraayrkkl ackplhrkvh rv
//
LOCUS       XP_077504668             210 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114662 [Amblyomma americanum].
ACCESSION   XP_077504668
VERSION     XP_077504668.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648542.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..210
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..210
                     /product="uncharacterized protein LOC144114662"
                     /calculated_mol_wt=23287
     Region          24..210
                     /region_name="uS7_Eukaryote"
                     /note="Eukaryota homolog of Ribosomal Protein S7; cd14867"
                     /db_xref="CDD:271246"
     Site            order(24,53..55,57..60,93,97)
                     /site_type="other"
                     /note="S9 interface [polypeptide binding]"
                     /db_xref="CDD:271246"
     Site            order(57,59,63..66,69,77,80,82..86,90..94,97,137..139,141,
                     151,158,165,168,174..175)
                     /site_type="other"
                     /note="rRNA binding site [nucleotide binding]"
                     /db_xref="CDD:271246"
     Site            order(101,104..105,108,174,176..177)
                     /site_type="other"
                     /note="S25 interface [polypeptide binding]"
                     /db_xref="CDD:271246"
     Site            order(136,139,141,210)
                     /site_type="other"
                     /note="S11 interface [polypeptide binding]"
                     /db_xref="CDD:271246"
     CDS             1..210
                     /gene="LOC144114662"
                     /coded_by="XM_077648542.1:95..727"
                     /db_xref="GeneID:144114662"
ORIGIN      
        1 mtdcwedtap ttvavvpteq letklfgrws ssdvqvsdig ltdyiavkek hakylphssg
       61 ryaskrfrka qcpiverltn smmmhgrnng kkllavkivk hsfeiihllt genplqvvvn
      121 aiinsgpred strigragtv rrqavdvspl rrvnqaiwll ctgaresafr niktiaecla
      181 delinaakgs snsyaikkkd elervaksnr
//
LOCUS       XP_077504669             210 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114662 [Amblyomma americanum].
ACCESSION   XP_077504669
VERSION     XP_077504669.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648543.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..210
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..210
                     /product="uncharacterized protein LOC144114662"
                     /calculated_mol_wt=23287
     Region          24..210
                     /region_name="uS7_Eukaryote"
                     /note="Eukaryota homolog of Ribosomal Protein S7; cd14867"
                     /db_xref="CDD:271246"
     Site            order(24,53..55,57..60,93,97)
                     /site_type="other"
                     /note="S9 interface [polypeptide binding]"
                     /db_xref="CDD:271246"
     Site            order(57,59,63..66,69,77,80,82..86,90..94,97,137..139,141,
                     151,158,165,168,174..175)
                     /site_type="other"
                     /note="rRNA binding site [nucleotide binding]"
                     /db_xref="CDD:271246"
     Site            order(101,104..105,108,174,176..177)
                     /site_type="other"
                     /note="S25 interface [polypeptide binding]"
                     /db_xref="CDD:271246"
     Site            order(136,139,141,210)
                     /site_type="other"
                     /note="S11 interface [polypeptide binding]"
                     /db_xref="CDD:271246"
     CDS             1..210
                     /gene="LOC144114662"
                     /coded_by="XM_077648543.1:124..756"
                     /db_xref="GeneID:144114662"
ORIGIN      
        1 mtdcwedtap ttvavvpteq letklfgrws ssdvqvsdig ltdyiavkek hakylphssg
       61 ryaskrfrka qcpiverltn smmmhgrnng kkllavkivk hsfeiihllt genplqvvvn
      121 aiinsgpred strigragtv rrqavdvspl rrvnqaiwll ctgaresafr niktiaecla
      181 delinaakgs snsyaikkkd elervaksnr
//
LOCUS       XP_077504670             406 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial ribosomal protein L3 [Amblyomma americanum].
ACCESSION   XP_077504670
VERSION     XP_077504670.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648544.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..406
                     /product="mitochondrial ribosomal protein L3"
                     /calculated_mol_wt=45909
     Region          128..337
                     /region_name="rpl3p"
                     /note="50S ribosomal protein L3P; Reviewed; cl00324"
                     /db_xref="CDD:444836"
     CDS             1..406
                     /gene="mRpL3"
                     /coded_by="XM_077648544.1:16..1236"
                     /db_xref="GeneID:144114663"
ORIGIN      
        1 mvglcslrsp carrmawyvk afsqiklfsg favhnasttq tftpclavac niqcryktst
       61 tikrrktypf hwwaprkrkv rdqehilpen yaflqelaqk kylqpgespl reepwpvatw
      121 tpesrrtgvi grkigiypmw tntgrrlltt liqvldnhvi dyippeqyak trfgyrdkgr
      181 gclvvgagsa dpmnfkapyl klfeksgvmp kkkltrflit pdaaippgtp ltaahfrpgd
      241 yvnvygktrg hgfqgvmkrw gmkggpathg ttkahrrlga iggprgiiqk gkrmpghmgq
      301 errlvvglrv wrintlhnvl fvqgpavpgh tlalvkvyds thakrahspe dpppfptyyp
      361 dperplpeem ydkelqpfea psitfedeev qvkkkakvka ktkgrk
//
LOCUS       XP_077504671             396 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114664 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504671
VERSION     XP_077504671.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648545.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..396
                     /product="uncharacterized protein LOC144114664 isoform X1"
                     /calculated_mol_wt=44418
     Region          198..330
                     /region_name="NTF2_like"
                     /note="Nuclear transport factor 2 (NTF2-like) superfamily.
                     This family includes members of the NTF2 family,
                     Delta-5-3-ketosteroid isomerases, Scytalone Dehydratases,
                     and the beta subunit of Ring hydroxylating dioxygenases.
                     This family is a classic example of...; cl09109"
                     /db_xref="CDD:471850"
     CDS             1..396
                     /gene="LOC144114664"
                     /coded_by="XM_077648545.1:129..1319"
                     /db_xref="GeneID:144114664"
ORIGIN      
        1 mavcfralkg sinsalgtvk tgnirshtgl ltlsattpcn cyvtltssqg tkalarhaav
       61 lphivagtsv qstcrsssic lpatrpqpei itndsvapel vrqeeqapft ffelrsgdde
      121 tkssgvvcrv pttvlsgyld aypykvycfd verrvaaema lsdyerevlq fagtsvalkn
      181 sgseqlpdpg rateqqlqri srvlaehlpr fftephhygl ynknivfrnn irgvttrgit
      241 gyvqqmalvr ilghlryahv rmevlkmtth kedstvrvrw rivgisglra lfmfwkfriw
      301 ewqkmisqea ewtdgfsvlk vgsdgliyeh icdkrqttsm ysgarkaglg rsarprrrvr
      361 lqkwrghelc avhqacslwq slsctngcah tqirny
//
LOCUS       XP_077504672             379 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114664 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504672
VERSION     XP_077504672.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648546.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..379
                     /product="uncharacterized protein LOC144114664 isoform X2"
                     /calculated_mol_wt=42094
     Region          198..330
                     /region_name="NTF2_like"
                     /note="Nuclear transport factor 2 (NTF2-like) superfamily.
                     This family includes members of the NTF2 family,
                     Delta-5-3-ketosteroid isomerases, Scytalone Dehydratases,
                     and the beta subunit of Ring hydroxylating dioxygenases.
                     This family is a classic example of...; cl09109"
                     /db_xref="CDD:471850"
     CDS             1..379
                     /gene="LOC144114664"
                     /coded_by="XM_077648546.1:129..1268"
                     /db_xref="GeneID:144114664"
ORIGIN      
        1 mavcfralkg sinsalgtvk tgnirshtgl ltlsattpcn cyvtltssqg tkalarhaav
       61 lphivagtsv qstcrsssic lpatrpqpei itndsvapel vrqeeqapft ffelrsgdde
      121 tkssgvvcrv pttvlsgyld aypykvycfd verrvaaema lsdyerevlq fagtsvalkn
      181 sgseqlpdpg rateqqlqri srvlaehlpr fftephhygl ynknivfrnn irgvttrgit
      241 gyvqqmalvr ilghlryahv rmevlkmtth kedstvrvrw rivgisglra lfmfwkfriw
      301 ewqkmisqea ewtdgfsvlk vgsdgliyeh icdkmmpdee leevkpsnva vklalllglt
      361 pqsfsfgspa lsssensll
//
LOCUS       XP_077504673             347 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114664 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504673
VERSION     XP_077504673.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648547.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..347
                     /product="uncharacterized protein LOC144114664 isoform X3"
                     /calculated_mol_wt=39449
     Region          149..281
                     /region_name="NTF2_like"
                     /note="Nuclear transport factor 2 (NTF2-like) superfamily.
                     This family includes members of the NTF2 family,
                     Delta-5-3-ketosteroid isomerases, Scytalone Dehydratases,
                     and the beta subunit of Ring hydroxylating dioxygenases.
                     This family is a classic example of...; cl09109"
                     /db_xref="CDD:471850"
     CDS             1..347
                     /gene="LOC144114664"
                     /coded_by="XM_077648547.1:147..1190"
                     /db_xref="GeneID:144114664"
ORIGIN      
        1 mqealarhaa vlphivagts vqstcrsssi clpatrpqpe iitndsvape lvrqeeqapf
       61 tffelrsgdd etkssgvvcr vpttvlsgyl daypykvycf dverrvaaem alsdyerevl
      121 qfagtsvalk nsgseqlpdp grateqqlqr isrvlaehlp rfftephhyg lynknivfrn
      181 nirgvttrgi tgyvqqmalv rilghlryah vrmevlkmtt hkedstvrvr wrivgisglr
      241 alfmfwkfri wewqkmisqe aewtdgfsvl kvgsdgliye hicdkrqtts mysgarkagl
      301 grsarprrrv rlqkwrghel cavhqacslw qslsctngca htqirny
//
LOCUS       XP_077504674             339 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114664 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077504674
VERSION     XP_077504674.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648548.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..339
                     /product="uncharacterized protein LOC144114664 isoform X4"
                     /calculated_mol_wt=38049
     Region          198..330
                     /region_name="NTF2_like"
                     /note="Nuclear transport factor 2 (NTF2-like) superfamily.
                     This family includes members of the NTF2 family,
                     Delta-5-3-ketosteroid isomerases, Scytalone Dehydratases,
                     and the beta subunit of Ring hydroxylating dioxygenases.
                     This family is a classic example of...; cl09109"
                     /db_xref="CDD:471850"
     CDS             1..339
                     /gene="LOC144114664"
                     /coded_by="XM_077648548.1:129..1148"
                     /db_xref="GeneID:144114664"
ORIGIN      
        1 mavcfralkg sinsalgtvk tgnirshtgl ltlsattpcn cyvtltssqg tkalarhaav
       61 lphivagtsv qstcrsssic lpatrpqpei itndsvapel vrqeeqapft ffelrsgdde
      121 tkssgvvcrv pttvlsgyld aypykvycfd verrvaaema lsdyerevlq fagtsvalkn
      181 sgseqlpdpg rateqqlqri srvlaehlpr fftephhygl ynknivfrnn irgvttrgit
      241 gyvqqmalvr ilghlryahv rmevlkmtth kedstvrvrw rivgisglra lfmfwkfriw
      301 ewqkmisqea ewtdgfsvlk vgsdgliyeh icdkrhywa
//
LOCUS       XP_077504675             430 aa            linear   INV 25-NOV-2025
DEFINITION  dual specificity protein phosphatase 12-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504675
VERSION     XP_077504675.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648549.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..430
                     /product="dual specificity protein phosphatase 12-like
                     isoform X1"
                     /calculated_mol_wt=48189
     Region          30..161
                     /region_name="DSP"
                     /note="dual-specificity phosphatase domain; cd14498"
                     /db_xref="CDD:350348"
     CDS             1..430
                     /gene="LOC144114665"
                     /coded_by="XM_077648549.1:211..1503"
                     /db_xref="GeneID:144114665"
ORIGIN      
        1 mssdtgtgdc sqssaeppdn pacpktmplc slvedglylg grdaaededl iqgfgithvl
       61 tvdtyrvdfe gnvvclylya edraeedlls rfneacdfie kgqqtgaclv hcrfgvsrsa
      121 tlvaahlmrk yaldftealh klkerrscig pnagfvaqlk lfqkmgykvd radlqfrlfv
      181 lerlshlakr agsfyavpce vksfwtdqdi ssgeclrckk crftlcftsk ivphtpgysi
      241 awwdnrwkep eerlcntsif veptawlfnq aralqgrltc pnchgklgny nwsglycecg
      301 acaqpgfhit pskvdralga sqpvkaylrp dkrntdepay srlqeepdsd tvctyeqlde
      361 epdssdaepi saccqleqqp endkselvca ysqleeeqqe arsienspvs afhqskeesq
      421 dggreyprmn
//
LOCUS       XP_077504676             370 aa            linear   INV 25-NOV-2025
DEFINITION  dual specificity protein phosphatase 12-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504676
VERSION     XP_077504676.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648550.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..370
                     /product="dual specificity protein phosphatase 12-like
                     isoform X2"
                     /calculated_mol_wt=41246
     Region          30..161
                     /region_name="DSP"
                     /note="dual-specificity phosphatase domain; cd14498"
                     /db_xref="CDD:350348"
     CDS             1..370
                     /gene="LOC144114665"
                     /coded_by="XM_077648550.1:134..1246"
                     /db_xref="GeneID:144114665"
ORIGIN      
        1 mssdtgtgdc sqssaeppdn pacpktmplc slvedglylg grdaaededl iqgfgithvl
       61 tvdtyrvdfe gnvvclylya edraeedlls rfneacdfie kgqqtgaclv hcrfgvsrsa
      121 tlvaahlmrk yaldftealh klkerrscig pnagfvaqlk lfqkmgykvd radlqfrlfv
      181 lerlshlakr agsfyavpce vksfwtdqdi ssgeclrckk crftlcftsk ivphtpgysi
      241 awwdnrwkep eerlcntsif veptawlfnq aralqgrgct asvvpvlsqa ftsrrarwtg
      301 llvhhslsrh tcgrtsailt slpivgykrs ptlilcaptn nwmknltvvm pslsllavsw
      361 sssqrmtrvs
//
LOCUS       XP_077504678             462 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114669 [Amblyomma americanum].
ACCESSION   XP_077504678
VERSION     XP_077504678.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648552.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..462
                     /product="uncharacterized protein LOC144114669"
                     /calculated_mol_wt=51005
     Region          224..289
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cd00590"
                     /db_xref="CDD:409669"
     CDS             1..462
                     /gene="LOC144114669"
                     /coded_by="XM_077648552.1:196..1584"
                     /db_xref="GeneID:144114669"
ORIGIN      
        1 mklkknqvvs tkaskagkek lhkandddgd aesdtqeylk vsatpnkkpk vpapeadsee
       61 ddldvddeel ddedddfgee gslddeeldd eesdglsged daevpqlvaa gkpkakqkvg
      121 gkeaavegsd ddddddgdee dddsnledee eeedddedde edekeesttt kakkpskdsg
      181 nkgqqtkrka ddgpieqisk kskqgdgipq svfmdideyd klflyvgnlp egctveevka
      241 lspdiigvrt nvkreklsfv fvefksekva ernlkllqaa klrgrplkvg yrgakradcp
      301 mtwrevqnel cvyglpqgvp qhrvarlfpt atqvttmafy tlvvfeskea lvdalknkac
      361 hsidgcplkf cyrlkadgkg kkrrsrkkre hgklldsnph msskgalgkk hlkkeenits
      421 agnkllqkkv ggaanartsk akfdakrkev kklaaaarqr kv
//
LOCUS       XP_077504679             351 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114670 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504679
VERSION     XP_077504679.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648553.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..351
                     /product="uncharacterized protein LOC144114670 isoform X1"
                     /calculated_mol_wt=38499
     Region          179..250
                     /region_name="RRM"
                     /note="RNA recognition motif (RRM) domain [Translation,
                     ribosomal structure and biogenesis]; COG0724"
                     /db_xref="CDD:440488"
     Region          <212..>347
                     /region_name="PABP-1234"
                     /note="polyadenylate binding protein, human types 1, 2, 3,
                     4 family; TIGR01628"
                     /db_xref="CDD:130689"
     CDS             1..351
                     /gene="LOC144114670"
                     /coded_by="XM_077648553.1:149..1204"
                     /db_xref="GeneID:144114670"
ORIGIN      
        1 mgpekdadlk qpdpsagsek pslgsegqet saggekaavp ttatqaaepa kqdadkaada
       61 nggqnavscs sgtaegtgae ghegprravn rangrvsegs lsgrpkrrkp rkegeslsqs
      121 epegtdresg ppsrssgsrg yyrnrtsrdr sdtrprvdmd esipeshryv waddmfkrsl
      181 yvgnmssqvt kedvralsda irsvrfvnrr safldfespe vasaqlellr smqlkgqplq
      241 vepsrprsss tyksdrllyv rgfpddkgmd algplfpdal lidrregkvl lrfkdhesai
      301 naikeviahd gtdfkfsfaa arprwnrrgp pravwrrhpp grfvkhearq d
//
LOCUS       XP_077504680             342 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114670 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504680
VERSION     XP_077504680.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648554.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..342
                     /product="uncharacterized protein LOC144114670 isoform X2"
                     /calculated_mol_wt=37665
     Region          170..241
                     /region_name="RRM"
                     /note="RNA recognition motif (RRM) domain [Translation,
                     ribosomal structure and biogenesis]; COG0724"
                     /db_xref="CDD:440488"
     Region          <203..>338
                     /region_name="PABP-1234"
                     /note="polyadenylate binding protein, human types 1, 2, 3,
                     4 family; TIGR01628"
                     /db_xref="CDD:130689"
     CDS             1..342
                     /gene="LOC144114670"
                     /coded_by="XM_077648554.1:138..1166"
                     /db_xref="GeneID:144114670"
ORIGIN      
        1 mgpekdadlk qpdpsagsek pslgsegqet saggekaavp ttatqaaepa kqdadkaada
       61 nggtaegtga eghegprrav nrangrvseg slsgrpkrrk prkegeslsq sepegtdres
      121 gppsrssgsr gyyrnrtsrd rsdtrprvdm desipeshry vwaddmfkrs lyvgnmssqv
      181 tkedvralsd airsvrfvnr rsafldfesp evasaqlell rsmqlkgqpl qvepsrprss
      241 styksdrlly vrgfpddkgm dalgplfpda llidrregkv llrfkdhesa inaikeviah
      301 dgtdfkfsfa aarprwnrrg ppravwrrhp pgrfvkhear qd
//
LOCUS       XP_077504681             310 aa            linear   INV 25-NOV-2025
DEFINITION  hydroxyacyl-coenzyme A dehydrogenase, mitochondrial-like [Amblyomma
            americanum].
ACCESSION   XP_077504681
VERSION     XP_077504681.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648555.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..310
                     /product="hydroxyacyl-coenzyme A dehydrogenase,
                     mitochondrial-like"
                     /calculated_mol_wt=33411
     Region          32..306
                     /region_name="FadB"
                     /note="3-hydroxyacyl-CoA dehydrogenase [Lipid transport
                     and metabolism]; COG1250"
                     /db_xref="CDD:440862"
     CDS             1..310
                     /gene="LOC144114671"
                     /coded_by="XM_077648555.1:85..1017"
                     /db_xref="GeneID:144114671"
ORIGIN      
        1 mlatylsfra fssssivrta ikhvvvvggg smgagiaqva sqtghqvvlv dvskevldks
       61 karieeslkr vakkkfaddk kageefvqkt lagiavstsa eeavkntdlv veaivenidv
      121 kkklfvaldk vagpltifss ntsslpiadi asstsrkdrf gglhffnpvp vmkllevvri
      181 petsedtfqk mqawgkalgk ttvickdtpg fivnrllvpa mleavrmler gdasaqdvdt
      241 amklgaghpm gpleladyvg ldvtkfiidg whqrfpenpl fkpsalldkl vqegklgvkt
      301 gegfykhskk
//
LOCUS       XP_077504682             221 aa            linear   INV 25-NOV-2025
DEFINITION  casein kinase II subunit beta [Amblyomma americanum].
ACCESSION   XP_077504682
VERSION     XP_077504682.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648556.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..221
                     /product="casein kinase II subunit beta"
                     /calculated_mol_wt=25346
     Region          8..191
                     /region_name="CK_II_beta"
                     /note="Casein kinase II regulatory subunit; smart01085"
                     /db_xref="CDD:198153"
     CDS             1..221
                     /gene="LOC144114672"
                     /coded_by="XM_077648556.1:212..877"
                     /db_xref="GeneID:144114672"
ORIGIN      
        1 mssseevswi swfcglrgne ffcevdedyi qdkfnltgls eqvphyrqal dmildlepdd
       61 elddnpnqsd lieqaaemly gliharyilt nrgialmiek yqngdfgycp rvycesqqml
      121 piglsdvpge amvklycpkc mdvygpkssr hhhtdgayfg tgfphmlfmv hpeyrpkrpt
      181 nqfvprlygf kihplayqlq qqvaanfkap irgmnygngk r
//
LOCUS       XP_077504683              90 aa            linear   INV 25-NOV-2025
DEFINITION  homeobox protein CHOX-CAD2-like [Amblyomma americanum].
ACCESSION   XP_077504683
VERSION     XP_077504683.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648557.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..90
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..90
                     /product="homeobox protein CHOX-CAD2-like"
                     /calculated_mol_wt=10762
     Region          22..79
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..90
                     /gene="LOC144114673"
                     /coded_by="XM_077648557.1:348..620"
                     /db_xref="GeneID:144114673"
ORIGIN      
        1 mltshlclkl plkppdivll grtrtferys ayqrsqleqe yqrsrfisye rkaelaqrlg
       61 lsktqvqfwf qnrrskekkl lqknmnafqp
//
LOCUS       XP_077504684             589 aa            linear   INV 25-NOV-2025
DEFINITION  ftz transcription factor 1 [Amblyomma americanum].
ACCESSION   XP_077504684
VERSION     XP_077504684.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648558.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..589
                     /product="ftz transcription factor 1"
                     /calculated_mol_wt=64100
     Region          71..163
                     /region_name="NR_DBD_Lrh-1_like"
                     /note="The DNA-binding domain of Lrh-1 like nuclear
                     receptor family like is composed of two C4-type zinc
                     fingers; cd07167"
                     /db_xref="CDD:143541"
     Site            order(71,74,88,91,107,113,123,126)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143541"
     Site            order(81..83,89..90,92,94,96..97,100,120..121,127,
                     140..142,145..151,157)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143541"
     Region          355..587
                     /region_name="NR_LBD_Ftz-F1_like"
                     /note="The ligand binding domain of FTZ-F1 like nuclear
                     receptors; cd06944"
                     /db_xref="CDD:132742"
     Site            order(393,396..397,433..434,437,441,456,475..476,561,
                     564..565,568)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132742"
     Site            order(405,408,412,422,425..426,429..430,578..579,582..583)
                     /site_type="other"
                     /note="coactivator recognition site [polypeptide binding]"
                     /db_xref="CDD:132742"
     CDS             1..589
                     /gene="ftz-f1"
                     /coded_by="XM_077648558.1:159..1928"
                     /db_xref="GeneID:144114675"
ORIGIN      
        1 mlvdgladtc sastmssdns nsssngnsaa alpvvssgav lsssgpppqp pphgdgypda
       61 pdtkegieel cpvcgdkvsg yhyglltces ckgffkrtvq nkkvytcvad rschidksqr
      121 krcpfcrfqk cldvgmklea vradrmrggr nkfgpmykrd rarklqmlrq kqlalsqcss
      181 aggseasgag gplavgsagv hyalppsasv sspglyspep ggpikqeiqi pqlssstssp
      241 dsspsplaal vgqpmalvpq phptqqqqqq hqaqqqqqqq qqqqqgpppp saatqqshya
      301 aaaaaaaaaa qpdktappqa pptpawltqg pssppkpfpa ayepgplvag ddakvpalvr
      361 efqknmlddk ewqtqlfgll qnqtynqcev dlfelmckvi dqslfaqvdw arstiffkdl
      421 rvddqmkllq qswsdmlild hvhqrlhngl pddttlpngq kfslvslall glpaladqlh
      481 alaarlqelr fdpvdyvclk fllllnpdvr tlsnvrlvhe aqeqtkqvhl eyclnnypqi
      541 tdkfgqlyqm lpnlklistr geeylfykhm sgsapsqtll memlhakkk
//
LOCUS       XP_077504685             295 aa            linear   INV 25-NOV-2025
DEFINITION  exosome complex component Rrp4 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504685
VERSION     XP_077504685.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648559.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..295
                     /product="exosome complex component Rrp4 isoform X1"
                     /calculated_mol_wt=32551
     Region          30..69
                     /region_name="ECR1_N"
                     /note="Exosome complex exonuclease RRP4 N-terminal region;
                     pfam14382"
                     /db_xref="CDD:464162"
     Region          81..172
                     /region_name="S1_Rrp4"
                     /note="Rrp4 S1-like RNA-binding domain. S1-like
                     RNA-binding domains are found in a wide variety of
                     RNA-associated proteins. Rrp4 protein is a subunit of the
                     exosome complex. The exosome plays a central role in 3' to
                     5' RNA processing and degradation in...; cd05789"
                     /db_xref="CDD:240215"
     Site            order(81,93,95,109)
                     /site_type="other"
                     /note="subunit interface [polypeptide binding]"
                     /db_xref="CDD:240215"
     Site            order(95,103,113,115)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:240215"
     Region          175..287
                     /region_name="KH-I_Rrp4_eukar"
                     /note="type I K homology (KH) RNA-binding domain found in
                     exosome complex component Rrp4 from eukaryote; cd22525"
                     /db_xref="CDD:411953"
     Site            order(175,184,187,189..190,192,210,231,234,237..238,
                     241..242,250..251,271..272)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:411953"
     CDS             1..295
                     /gene="Rrp4"
                     /coded_by="XM_077648559.1:37..924"
                     /db_xref="GeneID:144114677"
ORIGIN      
        1 matmatmavd vrlfsarkqd vrenarpkgl vapgdvittd lsfmrghgty vdssneqlls
       61 svagvvekvn klitvrplkt ryngeigdvv igrivevqqr rwkvemasrl naslllssvn
      121 lpggelrrks akdellmrqy lcegdlisae vqsvfadgsl slhtrslkyg klgqgtlvlv
      181 spslikrcki hfhslpcgvq vilgnngyvw icplpseegt kffqsvdmvp evtreaisrv
      241 rncivalarn hvllfdtsiv yafdislehk vnaltkpevm kevadltkqk lfvea
//
LOCUS       XP_077504686             264 aa            linear   INV 25-NOV-2025
DEFINITION  exosome complex component Rrp4 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504686
VERSION     XP_077504686.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648560.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..264
                     /product="exosome complex component Rrp4 isoform X2"
                     /calculated_mol_wt=29218
     Region          15..>77
                     /region_name="PRK09521"
                     /note="exosome complex RNA-binding protein Csl4;
                     Provisional"
                     /db_xref="CDD:236547"
     Region          50..141
                     /region_name="S1_Rrp4"
                     /note="Rrp4 S1-like RNA-binding domain. S1-like
                     RNA-binding domains are found in a wide variety of
                     RNA-associated proteins. Rrp4 protein is a subunit of the
                     exosome complex. The exosome plays a central role in 3' to
                     5' RNA processing and degradation in...; cd05789"
                     /db_xref="CDD:240215"
     Site            order(50,62,64,78)
                     /site_type="other"
                     /note="subunit interface [polypeptide binding]"
                     /db_xref="CDD:240215"
     Site            order(64,72,82,84)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:240215"
     Region          144..256
                     /region_name="KH-I_Rrp4_eukar"
                     /note="type I K homology (KH) RNA-binding domain found in
                     exosome complex component Rrp4 from eukaryote; cd22525"
                     /db_xref="CDD:411953"
     Site            order(144,153,156,158..159,161,179,200,203,206..207,
                     210..211,219..220,240..241)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:411953"
     CDS             1..264
                     /gene="Rrp4"
                     /coded_by="XM_077648560.1:16..810"
                     /db_xref="GeneID:144114677"
ORIGIN      
        1 mqirvflsls kankghgtyv dssneqllss vagvvekvnk litvrplktr yngeigdvvi
       61 grivevqqrr wkvemasrln aslllssvnl pggelrrksa kdellmrqyl cegdlisaev
      121 qsvfadgsls lhtrslkygk lgqgtlvlvs pslikrckih fhslpcgvqv ilgnngyvwi
      181 cplpseegtk ffqsvdmvpe vtreaisrvr ncivalarnh vllfdtsivy afdislehkv
      241 naltkpevmk evadltkqkl fvea
//
LOCUS       XP_077504687            1001 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114678 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504687
VERSION     XP_077504687.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648561.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1001
                     /product="uncharacterized protein LOC144114678 isoform X1"
                     /calculated_mol_wt=112736
     Region          58..134
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          339..>481
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..1001
                     /gene="LOC144114678"
                     /coded_by="XM_077648561.1:215..3220"
                     /db_xref="GeneID:144114678"
ORIGIN      
        1 mtqrvcaasa aagnaaaqwq qttatgasia klrerlptrr ahwtsvitvl svmshmycca
       61 kwcstsgksg ehlfrlpsdh rfsiwlkyan rlelletpre kvyktyrlcs khftrdcfts
      121 dactrlthea vpsvkvhepr lralvhpdfy eggaaqgies asplqstaap vevmlghdyl
      181 plpstlglgt iagadsavhd tddphssrap qltdvqttpp ppadeegaaq stpirviasa
      241 swdspdyaea gpsglsgake svrqpvmtpa sgtpksrrrk gavvytprtk krfeklhsrs
      301 dryrkalsri rkrhdtrrvt qqealnklep hlpqdlfelv kaqirlcsfp kskkhwskqi
      361 kqfalnlffh spkayrflre klhlphvrtl rrwladipmt pgiipgamda iesitkdwpl
      421 qdkaccllfd eislkknlmy dqskdivigf tddgntrtsr vanialvmav sgisrswmqp
      481 vafaashnat svlamrklll dliqqlqtkg llvkavicdq gsnnvslsrm lihsihepyf
      541 vvnnrkiyfm fdtphlikct rnnlrrhklt igsevvdwth ietlykktrh idrasktpve
      601 hlksrlakkl keqhiyrmpf admrvkyatq vfsesvsval ltliaiqelp vdakftaeft
      661 ermdklfdcl nssalkkqdd klryamtegs ehhvflesci dwiaqwhfgg pldkqphtik
      721 gwqitikall llwtdlkadf dythlftrrl qqdplenffa ivrqkhgcne tpnvyqfvaa
      781 vkhiwigqlf klsqgncevt thafltnles vsavlspsst pstshsrqqg sgssdlqass
      841 qdapdivyen vvyhvagtlv ksfldqrtte ctcegtllaa dggslhgrhq ffallkengv
      901 pehlfqsiaa tseaclnyik kldsafrkei sccahlsnvs rnlvermpnq qavfcslgck
      961 ikflklfcrt rlhwhlsfln rsmtsktkqs aparkrrkla g
//
LOCUS       XP_077504689             231 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114678 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504689
VERSION     XP_077504689.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648563.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..231
                     /product="uncharacterized protein LOC144114678 isoform X2"
                     /calculated_mol_wt=25198
     Region          58..134
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..231
                     /gene="LOC144114678"
                     /coded_by="XM_077648563.1:215..910"
                     /db_xref="GeneID:144114678"
ORIGIN      
        1 mtqrvcaasa aagnaaaqwq qttatgasia klrerlptrr ahwtsvitvl svmshmycca
       61 kwcstsgksg ehlfrlpsdh rfsiwlkyan rlelletpre kvyktyrlcs khftrdcfts
      121 dactrlthea vpsvkvhepr lralvhpdfy eggaaqgies asplqstaap vevmlghdyl
      181 plpstlglgt iagadsavhd tddphssrap qltdvqttpp ppadeevclf i
//
LOCUS       XP_077504690             917 aa            linear   INV 25-NOV-2025
DEFINITION  extracellular matrix organizing protein FRAS1-like [Amblyomma
            americanum].
ACCESSION   XP_077504690
VERSION     XP_077504690.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648564.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..917
                     /product="extracellular matrix organizing protein
                     FRAS1-like"
                     /calculated_mol_wt=100458
     CDS             1..917
                     /gene="LOC144114680"
                     /coded_by="XM_077648564.1:466..3219"
                     /db_xref="GeneID:144114680"
ORIGIN      
        1 mewakaaqhq rsslvlwlll lsvefgrcsr eapqghgaat fssaveqaaa rkavlpdnam
       61 qenflhtgal ekitdasakt fkspvlpava ddvdgvyiyp apvrsaesig herksssrtt
      121 akppssstip fvglpivtsl lhysdvrgya gqpvpagypv vcvppcdphh pdfavlgasc
      181 kgstrsgppd slsyeweaaa adaggsasfe pisretpfas vrqrvldsmy iasgrtvrcv
      241 vqetgdafgh rrtfsepvvi sdakglcpge kqqgqdvtat meyfngsdhg mqsgellisv
      301 eirhrdsmlp llfthplpsa eqifgnvisq kqhvcsnvaa pgghdlaffm qstaeatgag
      361 ggarpfqsdp kmrgsrtvsl yrhldldrcl wrfsaaislk davgicggvl ledfketesh
      421 rrfvtarlpl yvtlafpaaa tscgeaaprw msverrsqle ltfsyepalw serplgtraa
      481 pkakvlvtrm slgrdgrltv hlltralfhg mlslshatkk hvrgrflrpa agnaghysls
      541 lawtepsfda psqlwkavse drlkdysgph vlelvpcvaa paqeyrtppd dfctsfdslr
      601 fevpvralqp iittngavys mptkfellsr redfladprd lryshgdide kriffkgetl
      661 fgrvlwnpla elgaslrvdq vylcsgktgf tptfdptghe laggssfgcv qpspelehrf
      721 lildrtnpdn vqesvagvpf nakladddse asrlrdypgv dgfsmmvdpl yemasggqwy
      781 lqvlytvvph vrsrarrrrr dvfpcnetsa teahvfrigr qsrasgvvla rtvlatasta
      841 lfvlilgswv ayrrhkskap aapvhstrtr gcyravcghp eghhryhhcs tsltvwrvrv
      901 kpwtppsepi rpdvtev
//
LOCUS       XP_077504691             706 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: bZIP_ATF6 domain-containing protein ATf6
            [Amblyomma americanum].
ACCESSION   XP_077504691
VERSION     XP_077504691.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648565.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..706
                     /product="LOW QUALITY PROTEIN: bZIP_ATF6 domain-containing
                     protein ATf6"
                     /calculated_mol_wt=77835
     Region          302..352
                     /region_name="bZIP_ATF6"
                     /note="Basic leucine zipper (bZIP) domain of Activating
                     Transcription Factor-6 (ATF-6) and similar proteins: a
                     DNA-binding and dimerization domain; cd14700"
                     /db_xref="CDD:269848"
     Region          302..352
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269848"
     Site            order(305..306,308..310,312..317,319..321)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269848"
     Site            order(320,323..324,327..328,330..331,334..335,337..338,
                     341..342,344..345,348..349,351..352)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269848"
     CDS             1..706
                     /gene="Atf6"
                     /coded_by="XM_077648565.1:132..2252"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 1 base in 1 codon"
                     /db_xref="GeneID:144114681"
ORIGIN      
        1 mdvellalae sameikqeid rsfiadntfs qedynyifkd asdddiydri lhssaagmdg
       61 sqniekafci decsfvstgi kmemestrsy pdpdtflnnv qmtesnvlwg spnastvpet
      121 ppdtpptqsg sstpphlldc ptymagfsts shtasepvav qvpvtqpqva aplltvpspt
      181 ttmeaaqrtl mltraefdql taqgvftint atprdecsin tdavapkivs pralttkaak
      241 aplkitvpav salvsatnls svsptalpii ktqpvaianp alisgstnsl lqhindakal
      301 krqqrmiknr esaclsrkkr keylqklead vqdltsenar lkeenaelrq rvxqleaevs
      361 icflnikktt avmavmfmlt lnlapfsglf ysrmqsggar qvlpaaqspr wgrrllwsqs
      421 elpgegsnfv selqpavhds lsasnggeps pagflgalda qsnarngsfk cpssinqtes
      481 lrlqsqlrgw anhvehmnlq lteqrkkpsl aqrplvhrpr lqtwistryg sgaggsgvya
      541 ekweanrgqn yfqlyqalrr syeelyeaiq rkddtfyfvs fsgdylllsa nsenrtrmpr
      601 mslvmpagtf nesshmmkeh vpmvqidcev mstkimyvke svipphlrqq yprnttqaka
      661 sgsrlndasl qanetkpepg syprnvshhg kvqrqvprnh trrngq
//
LOCUS       XP_077504692             341 aa            linear   INV 25-NOV-2025
DEFINITION  NUBP iron-sulfur cluster assembly factor, mitochondrial isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077504692
VERSION     XP_077504692.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648566.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..341
                     /product="NUBP iron-sulfur cluster assembly factor,
                     mitochondrial isoform X1"
                     /calculated_mol_wt=36674
     Region          73..336
                     /region_name="ParA"
                     /note="NUBPL iron-transfer P-loop NTPase; pfam10609"
                     /db_xref="CDD:431392"
     Site            order(84..91,236,238,263..264,300..302,305..306)
                     /site_type="active"
                     /db_xref="CDD:349757"
     Site            order(84..86,117..118,120,123..124,182..183,213,215,218,
                     242,245,301,303..304,306)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:349757"
     CDS             1..341
                     /gene="Nubpl"
                     /coded_by="XM_077648566.1:89..1114"
                     /db_xref="GeneID:144114682"
ORIGIN      
        1 maarrcwlsi qlfarplyyr sytlrsenqf tgvyslcqst psrccgtdss akqaklnkeg
       61 asrglpkkfp vagvkhvvlv asgkggvgks ttavnlalal sldeskptvg lldadvygps
      121 iprmmnlsge peltqrafal dvpclqipis svenlmkplv nygikcmsmg flvdekvpvv
      181 wrglmvmsai ekllrqvawg pldylvvdmp pgtgdtqlsi sqnipvsgvv vvttpqeial
      241 idarkgaemf rkvnvpvlgl vqnmsrlacp scgheshifg kdgatelare maldilgdvp
      301 ldieiressd ngqpivvsna nspqaqvykd makkiigklt i
//
LOCUS       XP_077504693             324 aa            linear   INV 25-NOV-2025
DEFINITION  NUBP iron-sulfur cluster assembly factor, mitochondrial isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077504693
VERSION     XP_077504693.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648567.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..324
                     /product="NUBP iron-sulfur cluster assembly factor,
                     mitochondrial isoform X2"
                     /calculated_mol_wt=34862
     Region          73..319
                     /region_name="ParA"
                     /note="NUBPL iron-transfer P-loop NTPase; pfam10609"
                     /db_xref="CDD:431392"
     Site            order(84..91,219,221,246..247,283..285,288..289)
                     /site_type="active"
                     /db_xref="CDD:349757"
     Site            order(84..86,117..118,120,123..124,165..166,196,198,201,
                     225,228,284,286..287,289)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:349757"
     CDS             1..324
                     /gene="Nubpl"
                     /coded_by="XM_077648567.1:46..1020"
                     /db_xref="GeneID:144114682"
ORIGIN      
        1 maarrcwlsi qlfarplyyr sytlrsenqf tgvyslcqst psrccgtdss akqaklnkeg
       61 asrglpkkfp vagvkhvvlv asgkggvgks ttavnlalal sldeskptvg lldadvygps
      121 iprmmnlsge peltqqnlmk plvnygikcm smgflvdekv pvvwrglmvm saiekllrqv
      181 awgpldylvv dmppgtgdtq lsisqnipvs gvvvvttpqe ialidarkga emfrkvnvpv
      241 lglvqnmsrl acpscghesh ifgkdgatel aremaldilg dvpldieire ssdngqpivv
      301 snanspqaqv ykdmakkiig klti
//
LOCUS       XP_077504694             187 aa            linear   INV 25-NOV-2025
DEFINITION  frequenin-2-like [Amblyomma americanum].
ACCESSION   XP_077504694
VERSION     XP_077504694.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648568.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="frequenin-2-like"
                     /calculated_mol_wt=21774
     Region          40..174
                     /region_name="FRQ1"
                     /note="Ca2+-binding protein, EF-hand superfamily [Signal
                     transduction mechanisms]; COG5126"
                     /db_xref="CDD:444056"
     CDS             1..187
                     /gene="LOC144114683"
                     /coded_by="XM_077648568.1:190..753"
                     /db_xref="GeneID:144114683"
ORIGIN      
        1 mgkknsklkk evvnqlvnet yftekeirqw ykgfvkdcps ghlteqgflr iyklffpqgd
       61 pskftslifr vfdenkdgai efeefirals vtsrgnveek ldwafrlydv dsdgyitree
      121 mynivdaiyq mlgnqakdna eesprervdk ifeqldknhd nqltldefke gskqdpkivq
      181 alslysa
//
LOCUS       XP_077504695            1035 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114686 [Amblyomma americanum].
ACCESSION   XP_077504695
VERSION     XP_077504695.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648569.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1035
                     /product="uncharacterized protein LOC144114686"
                     /calculated_mol_wt=116607
     Region          <40..163
                     /region_name="EEP"
                     /note="Exonuclease-Endonuclease-Phosphatase (EEP) domain
                     superfamily; cl00490"
                     /db_xref="CDD:469791"
     Site            order(44,82,162)
                     /site_type="other"
                     /note="putative phosphate binding site [ion binding]"
                     /db_xref="CDD:197306"
     Region          444..711
                     /region_name="RT_nLTR_like"
                     /note="RT_nLTR: Non-LTR (long terminal repeat)
                     retrotransposon and non-LTR retrovirus reverse
                     transcriptase (RT). This subfamily contains both non-LTR
                     retrotransposons and non-LTR retrovirus RTs. RTs catalyze
                     the conversion of single-stranded RNA into...; cd01650"
                     /db_xref="CDD:238827"
     Site            order(533..538,639,641..642,707..708)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238827"
     Site            order(533..538,641)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238827"
     CDS             1..1035
                     /gene="LOC144114686"
                     /coded_by="XM_077648569.1:142..3249"
                     /db_xref="GeneID:144114686"
ORIGIN      
        1 mwrrdlewqa qecsdrvghd eaccdhlwvv gdllgvptal cvvylsvrss rpdtnrglve
       61 cvcrdahlva kdreviilgd fnghlseldg ytdangdlll wlaeqlqlni anldsrcngq
      121 ytwcarnsat cidyalvspr llslmqhlhi deeglvsmgs dhnrlrldft hthrrknrpq
      181 rskalylpvr qmevvakefe esqrrlgaat ygeymealra imrshmvhkg hrqaykkpww
      241 drqvaeawqa rrdanrahrr avkdkdqgaa avawdmylkr khemqslvqa klaaanlkem
      301 qtlraegksa aqkfwryvss ldrervgpte mvdsatgqpi tdlqqhvtdy lsglygppac
      361 mpedklegss psapsqdqpd iawevtlpgm nralgrisac tatgpdgipa rllkllgpns
      421 reqlaelftt iinggdiped wrngrvvlll kpggnaahig dyraltvtsv vyrvfmqvlk
      481 awisgwaesa hvltelqngf rpgrrlednl fvltscteia rkegrrlicc fldvekaydn
      541 vphetlfhrl gtlgmappll etikrlygen vvtavfgnvh servyvhrgl rqgcplspll
      601 yllyvaglek allqanlgfg lkysttgidn ssrlpglafa ddlvimgent edvqkmldic
      661 aaelrslglk fnqrkcrvvq lageandqls ltlneetltv drsykylgin lcaetdmfrl
      721 heakvrqsal raqcilrrrc lwgchqytmv rdlwklahvp gltfanavvc lpsatrnwle
      781 rrqtevgrta vgchgrvana aiqgdlgwss feareacskl eyrgrlqfms hnrwarrvfe
      841 ylaatclrtt wvkrlhkieg kyglfqtpis aesatawkqe arrrvqeken rqwlqalaek
      901 ptlavyregk nsigpekifd nglgsallfe aragalrtlv yrrhfdatpe aqaaicracg
      961 daeetiehlv mscqrlhpmp tegttfpqal gfhgpavdde gdsskvkseg vlrtkrrltl
     1021 wwstvfksak pqsrk
//
LOCUS       XP_077504696             187 aa            linear   INV 25-NOV-2025
DEFINITION  frequenin-1-like [Amblyomma americanum].
ACCESSION   XP_077504696
VERSION     XP_077504696.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648570.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="frequenin-1-like"
                     /calculated_mol_wt=21772
     Region          40..174
                     /region_name="FRQ1"
                     /note="Ca2+-binding protein, EF-hand superfamily [Signal
                     transduction mechanisms]; COG5126"
                     /db_xref="CDD:444056"
     CDS             1..187
                     /gene="LOC144114687"
                     /coded_by="XM_077648570.1:318..881"
                     /db_xref="GeneID:144114687"
ORIGIN      
        1 mgkknsklkg evvarlaket yftekevrqw ykgflkdcpn gllteqgflr iykqffprgd
       61 pskfaslvfr vfdenkdgsi efeefirals vtsrgnveek llwafklydv dndgfitree
      121 mynivdaiye mlgsqekede eddprarvdr ifeqldknqd nklsleefke gskhdpkivq
      181 alslyap
//
LOCUS       XP_077504697            1360 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114688 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504697
VERSION     XP_077504697.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648571.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1360
                     /product="uncharacterized protein LOC144114688 isoform X1"
                     /calculated_mol_wt=148343
     Region          3..87
                     /region_name="SANTA"
                     /note="SANTA (SANT Associated); pfam09133"
                     /db_xref="CDD:462689"
     Region          <959..1181
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     CDS             1..1360
                     /gene="LOC144114688"
                     /coded_by="XM_077648571.1:102..4184"
                     /db_xref="GeneID:144114688"
ORIGIN      
        1 mqrlrqwgiv psgtcemrie gyceangell itanvkrris rnrivcadgs syklvgkfdv
       61 isglrgrips rildkfvhgv psnwrqvvwg wsrpaentda giyrkdgnna srkqlciaqp
      121 rspkkrrdav lntpqlrprt rtksfrdgsv teipnlstak tqltiasgev kadafvkdvk
      181 vvveklplss isrlasatgv mkpsyntasv apspvvktrk qsaesnvpac trktrsgraa
      241 clqendrltr arkktattld mrrtsasrpp arasagtgtk tevcldtmls parsasawqn
      301 lsgdeqgtqg vcevyrmlea stpsklakrr mqdvpavrrk isqqrlacaa stpapsqakl
      361 tgqvprrwee rsngqglrkd dkeneepkpi narrpsnrnr icsdrevqra pevcqagsgq
      421 gvqtrqqsra stmelgekra prslcvlpfg hnlnldssln sassrketgv lrrgrsscdi
      481 vkkqqapart lrtnsnavis gqhlihatla angteppsgk rklrerslsg hqdvvaskkn
      541 vfstckslpv krkraasssp gmlpgkrrsa rilakaktak dvienqssar krkqsekpkg
      601 kvyalsnsan rkycrqlhkr elgasvglwq rnrkgesrke pginppdaak vdgsniteka
      661 asrpvlpmrv tpksvqlstk qefknddker ekpksqdser ssdekstcng hvmegvpehk
      721 gvglgaslse criagrsigp gdelafeslc tlparrrlkl dvphetaagr qeanvqcrth
      781 tlrsypvikq qkpaktrrrn sstvinshha mfktgntqqm sdqqnlgers lsghqnagas
      841 senilcdnqq vptmrkraaa lssgmpskkk tacnspevks agnagrkngs avsikrkqse
      901 qldqqvcsna antahyepkc paeargfacl tenkkvesqk psklgathvs nmgnsrivkp
      961 kaesspskkq alaskaqnqd sdkpkayssp skkqalaska qnqnsdkpka esspskkqal
     1021 askdqnqdsd kpkaysspsk kkalaskaqn qnsgkpkays spskkqalas kaqnqnsvkp
     1081 kaysnpskkq alaskaqnqn sdkpkaysnp skkqalaska qnqnsdkpka ksspskkqal
     1141 askaqnqnsv kskselspsk kqalaskaqn qdselkpsrs spqltkalla vtarkgtlkr
     1201 kaqirnlada laandacddf yhdrqqtevi psnffedcwd elsvcrlsgm ktptlnsplf
     1261 snprtpmsaa svcststlps ptamdaivqg iqkrekaval psitpkpkrk qqnlktvled
     1321 lqklekklkk shlkencads dedseesdsd sdsvfterpy
//
LOCUS       XP_077504699            1336 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114688 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504699
VERSION     XP_077504699.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648573.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1336
                     /product="uncharacterized protein LOC144114688 isoform X2"
                     /calculated_mol_wt=145758
     Region          3..87
                     /region_name="SANTA"
                     /note="SANTA (SANT Associated); pfam09133"
                     /db_xref="CDD:462689"
     Region          <932..>1179
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..1336
                     /gene="LOC144114688"
                     /coded_by="XM_077648573.1:102..4112"
                     /db_xref="GeneID:144114688"
ORIGIN      
        1 mqrlrqwgiv psgtcemrie gyceangell itanvkrris rnrivcadgs syklvgkfdv
       61 isglrgrips rildkfvhgv psnwrqvvwg wsrpaentda giyrkdgnna srkqlciaqp
      121 rspkkrrdav lntpqlrprt rtksfrdgsv teipnlstak tqltiasgev kadafvkdvk
      181 vvveklplss isrlasatgv mkpsyntasv apspvvktrk qsaesnvpac trktrsgraa
      241 clqendrltr arkktattld mrrtsasrpp arasagtgtk tevcldtmls parsasawqn
      301 lsgdeqgtqg vcevyrmlea stpsklakrr mqdvpavrrk isqqrlacaa stpapsqakl
      361 tgqvprrwee rsngqglrkd dkeneepkpi narrpsnrnr icsdrevqra pevcqagsgq
      421 gvqtrqqsra stmelgekra prslcvlpfg hnlnldssln sassrketgv lrrgrsscdi
      481 vkkqqapart lrtnsnavis gqhlihatla angteppsgk rklrerslsg hqdvvaskkn
      541 vfstckslpv krkraasssp gmlpgkrrsa rilakaktak dvienqssar krkqsekpkg
      601 kvyalsnsan rkycrqlhkr elgasvglwq rnrkgesrke pginppdaak vdgsniteka
      661 asrpvlpmrv tpksvqlstk qefknddker ekpksqdser ssdekstcng hvmegvpehk
      721 gvglgaslse criagrsigp gdelafeslc tlparrrlkl dvphetaagr qeanvqcrth
      781 tlrsypvikq qkpaktrrrn sstvinshha mfktgntqqm sdqqnlgers lsghqnagas
      841 senilcdnqq vptmrkraaa lssgmpskkk tacnspevks agnagrkngs avsikrkqse
      901 qldqqvcsna antahyepkc paeargfacl tenkkvesqk psklgathvs nmgnsrivkp
      961 kaesspskkq alaskaqnqd sdkpkayssp skkqalaska qnqnsdkpka esspskkqal
     1021 askdqnqdsd kpkaysspsk kkalaskaqn qnsgkpkays spskkqalas kaqnqnsvkp
     1081 kaysnpskkq alaskaqnqn sdkpkakssp skkqalaska qnqnsvksks elspskkqal
     1141 askaqnqdse lkpsrsspql tkallavtar kgtlkrkaqi rnladalaan dacddfyhdr
     1201 qqtevipsnf fedcwdelsv crlsgmktpt lnsplfsnpr tpmsaasvcs tstlpsptam
     1261 daivqgiqkr ekavalpsit pkpkrkqqnl ktvledlqkl ekklkkshlk encadsdeds
     1321 eesdsdsdsv fterpy
//
LOCUS       XP_077504700             443 aa            linear   INV 25-NOV-2025
DEFINITION  flotillin-1 [Amblyomma americanum].
ACCESSION   XP_077504700
VERSION     XP_077504700.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648574.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="flotillin-1"
                     /calculated_mol_wt=48972
     Region          4..420
                     /region_name="YqiK"
                     /note="Uncharacterized membrane protein YqiK, contains
                     Band7/PHB/SPFH domain [Function unknown]; COG2268"
                     /db_xref="CDD:441869"
     CDS             1..443
                     /gene="Flo1"
                     /coded_by="XM_077648574.1:488..1819"
                     /db_xref="GeneID:144114689"
ORIGIN      
        1 mvgfvacgpn ealvvsgcch ahpllvpggr vfvwpiiqrv qkislntmtl tiespkvytq
       61 qgvpisvtgi aqvkiqgqnv emlraaceqf lgkkddevmh iaretleghq raimgtmsve
      121 eiykdrkkfs kqvfevassd lvnmgitvvs ytikdisdde gylralgmar taevkrdari
      181 geaeaqrdsq ikealaeeer masrylndte iakaqrdfel kkaaydmevn tkkadsdlay
      241 slqaaktrqr ikeeqmqvqv iersqeiqvq eqeimrreke leatvrrpae aekyrlekma
      301 eanrnrvime aeaeaeavrl kgeaeafaie skaraeaeql ikkadafrey keaaildmml
      361 dtlpkvaaev aapisqckri vmvssgkgev gagkltgevi diitkttsmv hqltgvnvgs
      421 vcgkdrnlas agawntktaq aam
//
LOCUS       XP_077504701             300 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114691 [Amblyomma americanum].
ACCESSION   XP_077504701
VERSION     XP_077504701.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648575.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..300
                     /product="uncharacterized protein LOC144114691"
                     /calculated_mol_wt=33514
     CDS             1..300
                     /gene="LOC144114691"
                     /coded_by="XM_077648575.1:316..1218"
                     /db_xref="GeneID:144114691"
ORIGIN      
        1 msppvqrlrp htffghvpqg vcnvdickel vtrfsknels gmqdfgggrf kvtfrskpav
       61 qrflsnpiie vrgtavrfay rgtlstivrv fgfpvdidvs elrgaleqyg pvenialeyv
      121 pgfdsvpsgt rrarmemksa llnflqgaes tvqckyggva rvcrrcgsrd hvrstclcar
      181 aaacsatpra scpvpsaaat tlsrnarrap srllrsvrwl pllhrrfpsr qkitrillpp
      241 prlqsparsr parmsrcntw trraslpprr arrkrrrrda qrepsltgdd saqsedeasr
//
LOCUS       XP_077504702             300 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114691 [Amblyomma americanum].
ACCESSION   XP_077504702
VERSION     XP_077504702.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648576.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..300
                     /product="uncharacterized protein LOC144114691"
                     /calculated_mol_wt=33514
     CDS             1..300
                     /gene="LOC144114691"
                     /coded_by="XM_077648576.1:316..1218"
                     /db_xref="GeneID:144114691"
ORIGIN      
        1 msppvqrlrp htffghvpqg vcnvdickel vtrfsknels gmqdfgggrf kvtfrskpav
       61 qrflsnpiie vrgtavrfay rgtlstivrv fgfpvdidvs elrgaleqyg pvenialeyv
      121 pgfdsvpsgt rrarmemksa llnflqgaes tvqckyggva rvcrrcgsrd hvrstclcar
      181 aaacsatpra scpvpsaaat tlsrnarrap srllrsvrwl pllhrrfpsr qkitrillpp
      241 prlqsparsr parmsrcntw trraslpprr arrkrrrrda qrepsltgdd saqsedeasr
//
LOCUS       XP_077504703             300 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114691 [Amblyomma americanum].
ACCESSION   XP_077504703
VERSION     XP_077504703.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648577.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..300
                     /product="uncharacterized protein LOC144114691"
                     /calculated_mol_wt=33514
     CDS             1..300
                     /gene="LOC144114691"
                     /coded_by="XM_077648577.1:316..1218"
                     /db_xref="GeneID:144114691"
ORIGIN      
        1 msppvqrlrp htffghvpqg vcnvdickel vtrfsknels gmqdfgggrf kvtfrskpav
       61 qrflsnpiie vrgtavrfay rgtlstivrv fgfpvdidvs elrgaleqyg pvenialeyv
      121 pgfdsvpsgt rrarmemksa llnflqgaes tvqckyggva rvcrrcgsrd hvrstclcar
      181 aaacsatpra scpvpsaaat tlsrnarrap srllrsvrwl pllhrrfpsr qkitrillpp
      241 prlqsparsr parmsrcntw trraslpprr arrkrrrrda qrepsltgdd saqsedeasr
//
LOCUS       XP_077504704             300 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114691 [Amblyomma americanum].
ACCESSION   XP_077504704
VERSION     XP_077504704.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648578.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..300
                     /product="uncharacterized protein LOC144114691"
                     /calculated_mol_wt=33514
     CDS             1..300
                     /gene="LOC144114691"
                     /coded_by="XM_077648578.1:316..1218"
                     /db_xref="GeneID:144114691"
ORIGIN      
        1 msppvqrlrp htffghvpqg vcnvdickel vtrfsknels gmqdfgggrf kvtfrskpav
       61 qrflsnpiie vrgtavrfay rgtlstivrv fgfpvdidvs elrgaleqyg pvenialeyv
      121 pgfdsvpsgt rrarmemksa llnflqgaes tvqckyggva rvcrrcgsrd hvrstclcar
      181 aaacsatpra scpvpsaaat tlsrnarrap srllrsvrwl pllhrrfpsr qkitrillpp
      241 prlqsparsr parmsrcntw trraslpprr arrkrrrrda qrepsltgdd saqsedeasr
//
LOCUS       XP_077504705             300 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114691 [Amblyomma americanum].
ACCESSION   XP_077504705
VERSION     XP_077504705.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648579.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..300
                     /product="uncharacterized protein LOC144114691"
                     /calculated_mol_wt=33514
     CDS             1..300
                     /gene="LOC144114691"
                     /coded_by="XM_077648579.1:316..1218"
                     /db_xref="GeneID:144114691"
ORIGIN      
        1 msppvqrlrp htffghvpqg vcnvdickel vtrfsknels gmqdfgggrf kvtfrskpav
       61 qrflsnpiie vrgtavrfay rgtlstivrv fgfpvdidvs elrgaleqyg pvenialeyv
      121 pgfdsvpsgt rrarmemksa llnflqgaes tvqckyggva rvcrrcgsrd hvrstclcar
      181 aaacsatpra scpvpsaaat tlsrnarrap srllrsvrwl pllhrrfpsr qkitrillpp
      241 prlqsparsr parmsrcntw trraslpprr arrkrrrrda qrepsltgdd saqsedeasr
//
LOCUS       XP_077504706             139 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114692 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504706
VERSION     XP_077504706.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648580.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..139
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..139
                     /product="uncharacterized protein LOC144114692 isoform X1"
                     /calculated_mol_wt=15168
     CDS             1..139
                     /gene="LOC144114692"
                     /coded_by="XM_077648580.1:3813..4232"
                     /db_xref="GeneID:144114692"
ORIGIN      
        1 migvrvaeqq tpgmcrasrr dirvhwrtiw sqrcqqgsvn vcrerymtsp atlrcavpag
       61 egtstqeacv gsihdpfvwn ilsctarmei fklvmvcvkc rcggplspwd lsskalmpdp
      121 slltsrldcl pssvaipgs
//
LOCUS       XP_077504707             139 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114692 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504707
VERSION     XP_077504707.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648581.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..139
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..139
                     /product="uncharacterized protein LOC144114692 isoform X1"
                     /calculated_mol_wt=15168
     CDS             1..139
                     /gene="LOC144114692"
                     /coded_by="XM_077648581.1:321..740"
                     /db_xref="GeneID:144114692"
ORIGIN      
        1 migvrvaeqq tpgmcrasrr dirvhwrtiw sqrcqqgsvn vcrerymtsp atlrcavpag
       61 egtstqeacv gsihdpfvwn ilsctarmei fklvmvcvkc rcggplspwd lsskalmpdp
      121 slltsrldcl pssvaipgs
//
LOCUS       XP_077504708             123 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114692 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504708
VERSION     XP_077504708.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648582.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..123
                     /product="uncharacterized protein LOC144114692 isoform X2"
                     /calculated_mol_wt=13661
     CDS             1..123
                     /gene="LOC144114692"
                     /coded_by="XM_077648582.1:3813..4184"
                     /db_xref="GeneID:144114692"
ORIGIN      
        1 migvrvaeqq tpgmcrasrr dirvhwrtiw sqrcqqgsvn vcrerymtsp atlrcavpag
       61 egtstqeacv gsihdpfvwn ilsctarmei fklmwrtalt mgsqlespda rsifahvsag
      121 lft
//
LOCUS       XP_077504709             260 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114694 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504709
VERSION     XP_077504709.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648583.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..260
                     /product="uncharacterized protein LOC144114694 isoform X1"
                     /calculated_mol_wt=28730
     CDS             1..260
                     /gene="LOC144114694"
                     /coded_by="XM_077648583.1:775..1557"
                     /db_xref="GeneID:144114694"
ORIGIN      
        1 mkneydlqlv givkdepvfy sdsskdgvfd angsmknees easivgigrd nqhtgsdael
       61 tldratkqyr ekcdaanlrt wspnglfpkl pscvkpllhg tpmsaerhka llgrlrdeii
      121 qflndheliq dkntsvkhra ycalgrslat rypsmaweyv rpgtesyslq kncwsafmqr
      181 lsalrraqkr rmmrklqdaa attsstpaip ttpgtatnpk mpttpqisas lttptaptmp
      241 stpttmptps mvqvvvikqe
//
LOCUS       XP_077504710             260 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114694 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504710
VERSION     XP_077504710.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648584.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..260
                     /product="uncharacterized protein LOC144114694 isoform X1"
                     /calculated_mol_wt=28730
     CDS             1..260
                     /gene="LOC144114694"
                     /coded_by="XM_077648584.1:268..1050"
                     /db_xref="GeneID:144114694"
ORIGIN      
        1 mkneydlqlv givkdepvfy sdsskdgvfd angsmknees easivgigrd nqhtgsdael
       61 tldratkqyr ekcdaanlrt wspnglfpkl pscvkpllhg tpmsaerhka llgrlrdeii
      121 qflndheliq dkntsvkhra ycalgrslat rypsmaweyv rpgtesyslq kncwsafmqr
      181 lsalrraqkr rmmrklqdaa attsstpaip ttpgtatnpk mpttpqisas lttptaptmp
      241 stpttmptps mvqvvvikqe
//
LOCUS       XP_077504713             260 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114694 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504713
VERSION     XP_077504713.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648587.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..260
                     /product="uncharacterized protein LOC144114694 isoform X1"
                     /calculated_mol_wt=28730
     CDS             1..260
                     /gene="LOC144114694"
                     /coded_by="XM_077648587.1:674..1456"
                     /db_xref="GeneID:144114694"
ORIGIN      
        1 mkneydlqlv givkdepvfy sdsskdgvfd angsmknees easivgigrd nqhtgsdael
       61 tldratkqyr ekcdaanlrt wspnglfpkl pscvkpllhg tpmsaerhka llgrlrdeii
      121 qflndheliq dkntsvkhra ycalgrslat rypsmaweyv rpgtesyslq kncwsafmqr
      181 lsalrraqkr rmmrklqdaa attsstpaip ttpgtatnpk mpttpqisas lttptaptmp
      241 stpttmptps mvqvvvikqe
//
LOCUS       XP_077504714             259 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114694 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504714
VERSION     XP_077504714.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648588.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..259
                     /product="uncharacterized protein LOC144114694 isoform X2"
                     /calculated_mol_wt=28616
     CDS             1..259
                     /gene="LOC144114694"
                     /coded_by="XM_077648588.1:812..1591"
                     /db_xref="GeneID:144114694"
ORIGIN      
        1 mqeydlqlvg ivkdepvfys dsskdgvfda ngsmkneese asivgigrdn qhtgsdaelt
       61 ldratkqyre kcdaanlrtw spnglfpklp scvkpllhgt pmsaerhkal lgrlrdeiiq
      121 flndheliqd kntsvkhray calgrslatr ypsmaweyvr pgtesyslqk ncwsafmqrl
      181 salrraqkrr mmrklqdaaa ttsstpaipt tpgtatnpkm pttpqisasl ttptaptmps
      241 tpttmptpsm vqvvvikqe
//
LOCUS       XP_077504715             243 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114694 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504715
VERSION     XP_077504715.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648589.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..243
                     /product="uncharacterized protein LOC144114694 isoform X3"
                     /calculated_mol_wt=26906
     CDS             1..243
                     /gene="LOC144114694"
                     /coded_by="XM_077648589.1:766..1497"
                     /db_xref="GeneID:144114694"
ORIGIN      
        1 mkneydlqlv givkdepvfy sdsskdgvfd angsmkngsd aeltldratk qyrekcdaan
       61 lrtwspnglf pklpscvkpl lhgtpmsaer hkallgrlrd eiiqflndhe liqdkntsvk
      121 hraycalgrs latrypsmaw eyvrpgtesy slqkncwsaf mqrlsalrra qkrrmmrklq
      181 daaattsstp aipttpgtat npkmpttpqi saslttptap tmpstpttmp tpsmvqvvvi
      241 kqe
//
LOCUS       XP_077504716             242 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114694 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077504716
VERSION     XP_077504716.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648590.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..242
                     /product="uncharacterized protein LOC144114694 isoform X4"
                     /calculated_mol_wt=26792
     CDS             1..242
                     /gene="LOC144114694"
                     /coded_by="XM_077648590.1:803..1531"
                     /db_xref="GeneID:144114694"
ORIGIN      
        1 mqeydlqlvg ivkdepvfys dsskdgvfda ngsmkngsda eltldratkq yrekcdaanl
       61 rtwspnglfp klpscvkpll hgtpmsaerh kallgrlrde iiqflndhel iqdkntsvkh
      121 raycalgrsl atrypsmawe yvrpgtesys lqkncwsafm qrlsalrraq krrmmrklqd
      181 aaattsstpa ipttpgtatn pkmpttpqis aslttptapt mpstpttmpt psmvqvvvik
      241 qe
//
LOCUS       XP_077504717             185 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114694 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077504717
VERSION     XP_077504717.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648591.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..185
                     /product="uncharacterized protein LOC144114694 isoform X5"
                     /calculated_mol_wt=20219
     CDS             1..185
                     /gene="LOC144114694"
                     /coded_by="XM_077648591.1:802..1359"
                     /db_xref="GeneID:144114694"
ORIGIN      
        1 mkneydlqlv givkdepvfy sdsskdgvfd angsmknees easivgigrd nqhtgsdael
       61 tldratkqyr ekcdaanlrt wspnglfpkl pscvkpllhg tpmsaerhka llgrlrdeii
      121 qflktrtlqs sightarwvg pwlqgippwp gsmsgralrv tacrrtagal scnvsvlsdg
      181 prrge
//
LOCUS       XP_077504718             907 aa            linear   INV 25-NOV-2025
DEFINITION  stork-head domain-containing protein knockout [Amblyomma
            americanum].
ACCESSION   XP_077504718
VERSION     XP_077504718.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648592.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..907
                     /product="stork-head domain-containing protein knockout"
                     /calculated_mol_wt=97877
     Region          121..199
                     /region_name="Stork_head"
                     /note="Winged helix Storkhead-box1 domain; pfam10264"
                     /db_xref="CDD:431182"
     CDS             1..907
                     /gene="ko"
                     /coded_by="XM_077648592.1:212..2935"
                     /db_xref="GeneID:144114695"
ORIGIN      
        1 maprpedrrr clllgnclvv vcrrvgsddd qddatqdrag ppgwslfrgf vhenaqcfwn
       61 qclvqsvacl eyrgylipst ifvsgneytl evvrsawsrr vlrppvgyei qklgdvssve
      121 mvqvlqtqfa plaealckav fdltsrgsta tldtisaqlg qafpaierpg advvyralga
      181 lirerrlyht glgyqvaapd tflqtspspv pterpmlltn eeaisrlhgs pahttdesaa
      241 dgstlersas mrllrtraga sdeglalgsr lsrsssvrlc rsspahdgls paapsaaang
      301 krgahqdtga krdgkssmfs rwfrrsrsek rssgeckkql ssfsaqfppl ewsdpdyarf
      361 hsratqtsat tqpdthpyaa llkprssstn rarerhssng etpdasldrs drrsrssrgy
      421 yrgprkaaal aatedldssg tlmyrhrsas paarrnnhnq nngsrhggtl aqsstapsrr
      481 lpstaeirtp plsrrayrhq ralpplpver lnngglrqdd qryrppnftn nalekagage
      541 elppsgkgqq psdytnnnnn nrlpragpvp latfearrss iagttldidn vatsriraea
      601 qrhaptafsm knlqqqpsat atafpmrgga hisaddlknl hkaeekvepr piaaiknlqc
      661 gpmviskvpp qpqiieprrt tttttftttd tvtlkniqnl ssvadldktk pspqtlknlq
      721 pstntttvsi kqtngkiddd gaentkkiat nlileekiea qrksgaasps vagpikkive
      781 eksvqstles tgkmgnlkst vdvnmelvte vgssrtvtni ttslqhalpk pvaaeppsqe
      841 acnnksaaag kttkaskpak mngsgggytp traklaeaka affadsankg saddsgfvst
      901 spqpvpv
//
LOCUS       XP_077504719             517 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114696 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504719
VERSION     XP_077504719.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648593.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..517
                     /product="uncharacterized protein LOC144114696 isoform X1"
                     /calculated_mol_wt=58420
     CDS             1..517
                     /gene="LOC144114696"
                     /coded_by="XM_077648593.1:18..1571"
                     /db_xref="GeneID:144114696"
ORIGIN      
        1 mrsctsqddf rmkrglvssc idlfvsfgki aameleqris nwlpaldkei veltvqklqq
       61 cgveslehlk yvveedlqfl kpisrrille rlhsaatpnp pvslspndiq sptstqcasf
      121 stpwevfppq lmkacqegkr pvksdlnemi rlvsdhilai nrkpgrkilr aiaaeivshy
      181 petfedtlng avigsgietl lwklencvnn krrpsseqph qfepdceldl svkrvkiqrd
      241 sygcvawqpk lpsdetadsq ekkkddllsq frlafpdetm vaqlmsetya sqrqlinask
      301 nirdierswp flfqakhltn hvnillgsnv aktfddrlkt vgrqvfryah sqvkkecvks
      361 clqeieaakt nrksmaveye ampllllaif gedkelfykl teemasdddl gdlpscpfic
      421 akgwtfltan syticvdthp vlhaskpdea fkllffahfa fniqylkets lcleftqrai
      481 aginpargtk vqknggkqhc lsprvaalat alkdydf
//
LOCUS       XP_077504720             485 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114696 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504720
VERSION     XP_077504720.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648594.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..485
                     /product="uncharacterized protein LOC144114696 isoform X2"
                     /calculated_mol_wt=54868
     CDS             1..485
                     /gene="LOC144114696"
                     /coded_by="XM_077648594.1:1356..2813"
                     /db_xref="GeneID:144114696"
ORIGIN      
        1 meleqrisnw lpaldkeive ltvqklqqcg veslehlkyv veedlqflkp isrrillerl
       61 hsaatpnppv slspndiqsp tstqcasfst pwevfppqlm kacqegkrpv ksdlnemirl
      121 vsdhilainr kpgrkilrai aaeivshype tfedtlngav igsgietllw klencvnnkr
      181 rpsseqphqf epdceldlsv krvkiqrdsy gcvawqpklp sdetadsqek kkddllsqfr
      241 lafpdetmva qlmsetyasq rqlinaskni rdierswpfl fqakhltnhv nillgsnvak
      301 tfddrlktvg rqvfryahsq vkkecvkscl qeieaaktnr ksmaveyeam pllllaifge
      361 dkelfyklte emasdddlgd lpscpficak gwtfltansy ticvdthpvl haskpdeafk
      421 llffahfafn iqylketslc leftqraiag inpargtkvq knggkqhcls prvaalatal
      481 kdydf
//
LOCUS       XP_077504721             468 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114697 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504721
VERSION     XP_077504721.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648595.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..468
                     /product="uncharacterized protein LOC144114697 isoform X1"
                     /calculated_mol_wt=51677
     Region          <3..53
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          354..>458
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..468
                     /gene="LOC144114697"
                     /coded_by="XM_077648595.1:352..1758"
                     /db_xref="GeneID:144114697"
ORIGIN      
        1 mftlhrfpts palrqqyiaa vnrknfdate asrvcsrhfv ggkkteenvv pmldmgyekk
       61 vvvgrrlvrq tytpqpkkir rstgaktglc sdagqitdvg aqacpdlsms ddkdtdtple
      121 eyeaclapdd mveepksdqv atavvvpdma eeaildqvli ptmataetae emtedeltpa
      181 vaapemaddg ipdeaapame qspevrtast qwedqnnehs ycqrglgtls dkqqlsaatl
      241 geddcvfytg lgkthfqnlv tavkvelrkl ftvlpyedhl lltlmrlrlg llyghlarif
      301 qisvgsvcni frhvlsvlct imekvvtwlp rsitrnsmpk sfidngydtt tcivdcsein
      361 lqrpkklmpr aqtyssykah ntvkfliaia pdgyimyvsp ayggrasdky itkdckledh
      421 lgpgdevmad rgftlttsmi iqgvklnvpa ftkgksqlvk rkspgreg
//
LOCUS       XP_077504722             450 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114697 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504722
VERSION     XP_077504722.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648596.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..450
                     /product="uncharacterized protein LOC144114697 isoform X2"
                     /calculated_mol_wt=49923
     Region          <3..53
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          336..>440
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..450
                     /gene="LOC144114697"
                     /coded_by="XM_077648596.1:352..1704"
                     /db_xref="GeneID:144114697"
ORIGIN      
        1 mftlhrfpts palrqqyiaa vnrknfdate asrvcsrhfv ggkkteenvv pmldmgyekk
       61 vvvgrrlvrq tytpqpkkir rstgaktglc sdagqitdvg aqacpdlsms ddkdtdtple
      121 eyeaclapdd mveepksdqv atavvvpdma eeaildqvli ptmataetae emtedeltpa
      181 veqspevrta stqwedqnne hsycqrglgt lsdkqqlsaa tlgeddcvfy tglgkthfqn
      241 lvtavkvelr klftvlpyed hllltlmrlr lgllyghlar ifqisvgsvc nifrhvlsvl
      301 ctimekvvtw lprsitrnsm pksfidngyd tttcivdcse inlqrpkklm praqtyssyk
      361 ahntvkflia iapdgyimyv spayggrasd kyitkdckle dhlgpgdevm adrgftltts
      421 miiqgvklnv paftkgksql vkrkspgreg
//
LOCUS       XP_077504723             115 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114699 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504723
VERSION     XP_077504723.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648597.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..115
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..115
                     /product="uncharacterized protein LOC144114699 isoform X1"
                     /calculated_mol_wt=12225
     CDS             1..115
                     /gene="LOC144114699"
                     /coded_by="XM_077648597.1:1500..1847"
                     /db_xref="GeneID:144114699"
ORIGIN      
        1 mfslesnnmw lvnsqhaaeg glerlpttph lavigssmgd vkkiivavds trslekamgl
       61 llgaffvfnv ayppdcpltm ellqrylgqs nptkgrgkgr acgiapklls llnal
//
LOCUS       XP_077504724             115 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114699 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504724
VERSION     XP_077504724.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648598.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..115
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..115
                     /product="uncharacterized protein LOC144114699 isoform X1"
                     /calculated_mol_wt=12225
     CDS             1..115
                     /gene="LOC144114699"
                     /coded_by="XM_077648598.1:996..1343"
                     /db_xref="GeneID:144114699"
ORIGIN      
        1 mfslesnnmw lvnsqhaaeg glerlpttph lavigssmgd vkkiivavds trslekamgl
       61 llgaffvfnv ayppdcpltm ellqrylgqs nptkgrgkgr acgiapklls llnal
//
LOCUS       XP_077504725              78 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114699 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504725
VERSION     XP_077504725.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648599.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..78
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..78
                     /product="uncharacterized protein LOC144114699 isoform X2"
                     /calculated_mol_wt=8248
     CDS             1..78
                     /gene="LOC144114699"
                     /coded_by="XM_077648599.1:468..704"
                     /db_xref="GeneID:144114699"
ORIGIN      
        1 mgdvkkiiva vdstrsleka mglllgaffv fnvayppdcp ltmellqryl gqsnptkgrg
       61 kgracgiapk llsllnal
//
LOCUS       XP_077504726             495 aa            linear   INV 25-NOV-2025
DEFINITION  carboxypeptidase D-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504726
VERSION     XP_077504726.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648600.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..495
                     /product="carboxypeptidase D-like isoform X1"
                     /calculated_mol_wt=55712
     Region          58..366
                     /region_name="M14_CP_N-E_like"
                     /note="Peptidase M14 carboxypeptidase subfamily N/E-like;
                     cd03858"
                     /db_xref="CDD:349431"
     Site            order(120,123,183,192..193,247..248,254,258,311,315,317,
                     337)
                     /site_type="active"
                     /db_xref="CDD:349431"
     Region          370..456
                     /region_name="Peptidase_M14NE-CP-C_like"
                     /note="Peptidase associated domain: C-terminal domain of
                     M14 N/E carboxypeptidase; putative folding, regulation, or
                     interaction domain; cd11308"
                     /db_xref="CDD:200604"
     CDS             1..495
                     /gene="LOC144114701"
                     /coded_by="XM_077648600.1:134..1621"
                     /db_xref="GeneID:144114701"
ORIGIN      
        1 maglaepgqh rrrwpaphvp wpllavlvla lispadgvmr rdgeeqrsna hggfqfkhhd
       61 naevaeqlrr vsracpditn lyelghrsvl gqplwvleit drpgvheqle petkyvanmh
      121 gnevlgrelm lalawylcrq yregdqevsa llnstrihim psmnpdgwdt aarsaredwv
      181 sgranamgvd lnrdfpdler ilrekglrri kpdhlfkgel thpvqpetka vmewilslpf
      241 vlsanfhgga lvanypfddt ldgsqkkyts apddatfrhl ahtyasshpr mkqgemcggd
      301 afrdtggitn gaawyavagg mqdfnylgsn dfeitvelgc rkfppeselr kewednkqal
      361 lnflwqahig ikglvtdsls gepiadadve vtnvtggept lirhnvltse ngeywrllip
      421 gtymvrvyre gyvpaqkqvt veyrprteaq rvdfqlkpva aramemlles eaymdgsqeh
      481 rllpppqplk qmqpm
//
LOCUS       XP_077504727             377 aa            linear   INV 25-NOV-2025
DEFINITION  carboxypeptidase E-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504727
VERSION     XP_077504727.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648601.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..377
                     /product="carboxypeptidase E-like isoform X2"
                     /calculated_mol_wt=42421
     Region          <1..248
                     /region_name="Peptidase_M14_like"
                     /note="M14 family of metallocarboxypeptidases and related
                     proteins; cl11393"
                     /db_xref="CDD:472171"
     Site            order(2,5,65,74..75,129..130,136,219)
                     /site_type="active"
                     /db_xref="CDD:349431"
     Region          252..338
                     /region_name="Peptidase_M14NE-CP-C_like"
                     /note="Peptidase associated domain: C-terminal domain of
                     M14 N/E carboxypeptidase; putative folding, regulation, or
                     interaction domain; cd11308"
                     /db_xref="CDD:200604"
     CDS             1..377
                     /gene="LOC144114701"
                     /coded_by="XM_077648601.1:166..1299"
                     /db_xref="GeneID:144114701"
ORIGIN      
        1 mhgnevlgre lmlalawylc rqyregdqev sallnstrih impsmnpdgw dtaarsared
       61 wvsgranamg vdlnrdfpdl erilrekglr rikpdhlfkg elthpvqpet kavmewilsl
      121 pfvlsanfhg galvanypfd dtldgsqkky tsapddatfr hlahtyassh prmkqgemcg
      181 gdafrdtggi tngaawyava ggmqdfnylg sndfeitvel gcrkfppese lrkewednkq
      241 allnflwqah igikglvtds lsgepiadad vevtnvtgge ptlirhnvlt sengeywrll
      301 ipgtymvrvy regyvpaqkq vtveyrprte aqrvdfqlkp vaaramemll eseaymdgsq
      361 ehrllpppqp lkqmqpm
//
LOCUS       XP_077504728             401 aa            linear   INV 25-NOV-2025
DEFINITION  palmitoyltransferase ZDHHC6-like [Amblyomma americanum].
ACCESSION   XP_077504728
VERSION     XP_077504728.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648602.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..401
                     /product="palmitoyltransferase ZDHHC6-like"
                     /calculated_mol_wt=45597
     Region          96..229
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; pfam01529"
                     /db_xref="CDD:396215"
     CDS             1..401
                     /gene="LOC144114702"
                     /coded_by="XM_077648602.1:315..1520"
                     /db_xref="GeneID:144114702"
ORIGIN      
        1 mepqpgvldi laqfcqrlvh wgpvtallii kfifltslyv tsmwlspygs tlgtinhavf
       61 igwvgvllyn ffmavamgpg fvplkwrpeq pddeqflqyc ancdgfktpr shhcrkcerc
      121 vlkmdhhcpw intccghrnh anftlflffa vcgslhatvl lvmglskayh rkyymrqgqe
      181 dnlvylgfip flatvlslgl aigviiavgs lffiqlkiiv rnettienwi vakaqmrere
      241 needfiypyn lgvaenlkqv fftplgdgit wpvvpgcnqy tltveqilqk hdkrlrtrly
      301 tvvqpyrgsf fpishgctvc mttpltdepr iavspgdrvl vtrwkkhwly gelenakfst
      361 katcrlkgwf prqcavevag skgsrsctea sssyadhkky k
//
LOCUS       XP_077504729             289 aa            linear   INV 25-NOV-2025
DEFINITION  cyclin-dependent kinase 1-like [Amblyomma americanum].
ACCESSION   XP_077504729
VERSION     XP_077504729.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648603.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..289
                     /product="cyclin-dependent kinase 1-like"
                     /calculated_mol_wt=33310
     Region          4..288
                     /region_name="STKc_CDK1_CdkB_like"
                     /note="Catalytic domain of Cyclin-Dependent protein Kinase
                     1-like Serine/Threonine Kinases and of Plant B-type
                     Cyclin-Dependent protein Kinase; cd07835"
                     /db_xref="CDD:270829"
     Site            order(10..13,18,31,33,50,64,81..84,87,89..90,129,131,
                     133..134,136,147,150,162,164..167,169,207..208)
                     /site_type="active"
                     /db_xref="CDD:270829"
     Site            order(10..13,18,31,33,64,81..84,87,90,133..134,136,147)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270829"
     Site            order(37,42..44,46,49..50,52..53,56..57,71,118,121..124,
                     152..154,156..157,161..162,274,278..280)
                     /site_type="other"
                     /note="CDK/cyclin interface [polypeptide binding]"
                     /db_xref="CDD:270829"
     Site            order(50,89,129,131,150,162,164..167,169,207..208)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270829"
     Site            146..169
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270829"
     CDS             1..289
                     /gene="LOC144114703"
                     /coded_by="XM_077648603.1:212..1081"
                     /db_xref="GeneID:144114703"
ORIGIN      
        1 mdnyvkveki gegtygivyk gkdkrdgkiv alkkirlese degvpstair eiallkelkh
       61 kyivrledvl megsdkiylv feylsmdlkk yldgfdkner ldkglvksym sqileailfc
      121 hqrrvlhrdl kpqnllidnh gtikvadfgl arafgiplrv fthevvtlwy rapevllgsl
      181 rystpidiws igcifvemvt rrplfhgdse idqlfrifrt lgtpteqswp dvsklpdykp
      241 sfpswkenil asllpemdsd aldllnemli ynpakritar aalkhkyfd
//
LOCUS       XP_077504730             217 aa            linear   INV 25-NOV-2025
DEFINITION  sentrin-specific protease 8-like [Amblyomma americanum].
ACCESSION   XP_077504730
VERSION     XP_077504730.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648604.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="sentrin-specific protease 8-like"
                     /calculated_mol_wt=24715
     Region          29..173
                     /region_name="Peptidase_C48"
                     /note="Ulp1 protease family, C-terminal catalytic domain;
                     cl23802"
                     /db_xref="CDD:420019"
     CDS             1..217
                     /gene="LOC144114704"
                     /coded_by="XM_077648604.1:219..872"
                     /db_xref="GeneID:144114704"
ORIGIN      
        1 mashgrivls yhdtllresd velleeahwv ndnviwfwmq yienelfasr cgafafvgpd
       61 vvqlvklgaa lnvdqvlksl dlnqkalill pvndcqdfdl pggchwsflv ynqarkifeh
      121 ydsskghnhs hakaiarala pllslrkvrv teadclqqhn sfdcglyvmy nlqqvcaehi
      181 sraeddgspr tlltswmrhn rktlkelilt lskretk
//
LOCUS       XP_077504731             165 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114705 [Amblyomma americanum].
ACCESSION   XP_077504731
VERSION     XP_077504731.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648605.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..165
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..165
                     /product="uncharacterized protein LOC144114705"
                     /calculated_mol_wt=18255
     CDS             1..165
                     /gene="LOC144114705"
                     /coded_by="XM_077648605.1:314..811"
                     /db_xref="GeneID:144114705"
ORIGIN      
        1 maspdentps frqwlarlpp ftadnvykvy lpiagavsyc tfsvtvfipe llsrlypdqs
       61 vtvancllfn shlgtglyvy frphmarapp yhrimysayq avlfnfgsvl lwavtksllp
      121 nsnlvrslfa astsacflai gmeylefldt pgeedfsasa tsprc
//
LOCUS       XP_077504732            1389 aa            linear   INV 25-NOV-2025
DEFINITION  Ig-like and fibronectin type-III domain-containing protein 2
            [Amblyomma americanum].
ACCESSION   XP_077504732
VERSION     XP_077504732.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648606.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1389
                     /product="Ig-like and fibronectin type-III
                     domain-containing protein 2"
                     /calculated_mol_wt=152480
     Region          31..126
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          41..45
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          54..58
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          93..97
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          107..112
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          119..122
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          145..235
                     /region_name="DB"
                     /note="DB module; pfam01682"
                     /db_xref="CDD:460293"
     Region          243..353
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(243,326,341)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(342..343,345..346)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          383..474
                     /region_name="DB"
                     /note="DB module; pfam01682"
                     /db_xref="CDD:460293"
     Region          482..573
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(482,546,561)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(562..563,565..566)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          592..683
                     /region_name="DB"
                     /note="DB module; pfam01682"
                     /db_xref="CDD:460293"
     Region          <691..1051
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Region          1098..1184
                     /region_name="DB"
                     /note="DB module; pfam01682"
                     /db_xref="CDD:460293"
     Region          1192..1279
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(1192,1254,1269)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(1270..1271,1273..1274)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     CDS             1..1389
                     /gene="LOC144114707"
                     /coded_by="XM_077648606.1:580..4749"
                     /db_xref="GeneID:144114707"
ORIGIN      
        1 mqvcvprllv alvfatplna agpslqdtpl epvlavkgde alidcvvkdq anytvlwrrv
       61 sdrdkgavlt agnvrvigdp risvlhnpeh nnwvlrisnv qpldsgrysc elntspnqri
      121 trlltvleda rrsapfllth nytdccnerg ippecfgyct lqgivtgrhh sprtcleyig
      181 iltqcltdgr nhmpccvaqn ipqicrsvci gdytlttvte hytcmdytmt tlaciangve
      241 llpgppkqle vepvsptell irwmpphqei evthfrinit elqsfdpdav tpknetdkds
      301 tvgttqafsi kvdgnqtmfn lrnlkpvtmy evtvtsenmh gtslptyavr tltlgaepsf
      361 dlptpenits ndtllsipdv rgccvkrgvk qerclrtmcd psradettlt dvmicapwan
      421 vtfacmadgv dhtgccrrrg lpeaclgfck gsvmrvdyrh frcleympfy gscllehygv
      481 lpgppkeltv taisrywavl twkppsklpe tvtkyglfwr rlgddfepyf avpnvkppyl
      541 mdrlepssdy evfivaenky gtsegsdriv lrtqdpepep qdmpsgynet accvnakvkd
      601 icmplcsykm kladlqylsp lcldemhtll rcgaggrdhr pccqrygvrd ecqpicagev
      661 slskydvtal vcsrdigavl kcmeegkdii pgppedfhvt fvtptsvhlv wnapagnvta
      721 tqyqiryeai ngtvpphpae ytqevnvttt maivdkltpk tsysfyvvaa neqgfsvpsl
      781 iillttpekg vdekgiqsmv sppygvevlh aavdaialkw lpplhlppqs nlsyivrfap
      841 vnvsnddsrw tsvatpytsl iignltfntq yaisimamsg gkasvpsetv yawtdaaipa
      901 fvnlplvips gpimegvnmt vmcigvgvpt ptvsvyvngi lqiqlpqhhv aitvprvsrn
      961 vtkvscfatn gyghgahssv dihvissplv tvplkkayse mganarllce vsghpeprfl
     1021 wyrdsqlrnp lgrsdhidlq sspdplkphn yqsyliikgv taddggayfc saenqhgsma
     1081 gtvtlsvmpr sarnttncca slgvsaqcls acafdidinf avnrpqcikd ldklmhcaad
     1141 gsdhrqccrt kgvpssclrw cvgrpvfqtt qcalaaakei vscfeegkam lpgppqnvra
     1201 tqvapgsvdi ywdpplknpd vvqwyrvlwr svgsqsinrn etlkppfrfr dmdqgliyef
     1261 lvkagnhygm sastlpvvih ppgsaalagh glslggkiai vlvmtalvlv iaavlgifyw
     1321 krwkakarpp gvsfenptyl kdgvvlqnts gegtakpget tvengghrng rpravencye
     1381 diravrlss
//
LOCUS       XP_077504733            1389 aa            linear   INV 25-NOV-2025
DEFINITION  Ig-like and fibronectin type-III domain-containing protein 2
            [Amblyomma americanum].
ACCESSION   XP_077504733
VERSION     XP_077504733.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648607.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1389
                     /product="Ig-like and fibronectin type-III
                     domain-containing protein 2"
                     /calculated_mol_wt=152480
     Region          31..126
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          41..45
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          54..58
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          93..97
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          107..112
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          119..122
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          145..235
                     /region_name="DB"
                     /note="DB module; pfam01682"
                     /db_xref="CDD:460293"
     Region          243..353
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(243,326,341)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(342..343,345..346)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          383..474
                     /region_name="DB"
                     /note="DB module; pfam01682"
                     /db_xref="CDD:460293"
     Region          482..573
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(482,546,561)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(562..563,565..566)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          592..683
                     /region_name="DB"
                     /note="DB module; pfam01682"
                     /db_xref="CDD:460293"
     Region          <691..1051
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Region          1098..1184
                     /region_name="DB"
                     /note="DB module; pfam01682"
                     /db_xref="CDD:460293"
     Region          1192..1279
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(1192,1254,1269)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(1270..1271,1273..1274)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     CDS             1..1389
                     /gene="LOC144114707"
                     /coded_by="XM_077648607.1:586..4755"
                     /db_xref="GeneID:144114707"
ORIGIN      
        1 mqvcvprllv alvfatplna agpslqdtpl epvlavkgde alidcvvkdq anytvlwrrv
       61 sdrdkgavlt agnvrvigdp risvlhnpeh nnwvlrisnv qpldsgrysc elntspnqri
      121 trlltvleda rrsapfllth nytdccnerg ippecfgyct lqgivtgrhh sprtcleyig
      181 iltqcltdgr nhmpccvaqn ipqicrsvci gdytlttvte hytcmdytmt tlaciangve
      241 llpgppkqle vepvsptell irwmpphqei evthfrinit elqsfdpdav tpknetdkds
      301 tvgttqafsi kvdgnqtmfn lrnlkpvtmy evtvtsenmh gtslptyavr tltlgaepsf
      361 dlptpenits ndtllsipdv rgccvkrgvk qerclrtmcd psradettlt dvmicapwan
      421 vtfacmadgv dhtgccrrrg lpeaclgfck gsvmrvdyrh frcleympfy gscllehygv
      481 lpgppkeltv taisrywavl twkppsklpe tvtkyglfwr rlgddfepyf avpnvkppyl
      541 mdrlepssdy evfivaenky gtsegsdriv lrtqdpepep qdmpsgynet accvnakvkd
      601 icmplcsykm kladlqylsp lcldemhtll rcgaggrdhr pccqrygvrd ecqpicagev
      661 slskydvtal vcsrdigavl kcmeegkdii pgppedfhvt fvtptsvhlv wnapagnvta
      721 tqyqiryeai ngtvpphpae ytqevnvttt maivdkltpk tsysfyvvaa neqgfsvpsl
      781 iillttpekg vdekgiqsmv sppygvevlh aavdaialkw lpplhlppqs nlsyivrfap
      841 vnvsnddsrw tsvatpytsl iignltfntq yaisimamsg gkasvpsetv yawtdaaipa
      901 fvnlplvips gpimegvnmt vmcigvgvpt ptvsvyvngi lqiqlpqhhv aitvprvsrn
      961 vtkvscfatn gyghgahssv dihvissplv tvplkkayse mganarllce vsghpeprfl
     1021 wyrdsqlrnp lgrsdhidlq sspdplkphn yqsyliikgv taddggayfc saenqhgsma
     1081 gtvtlsvmpr sarnttncca slgvsaqcls acafdidinf avnrpqcikd ldklmhcaad
     1141 gsdhrqccrt kgvpssclrw cvgrpvfqtt qcalaaakei vscfeegkam lpgppqnvra
     1201 tqvapgsvdi ywdpplknpd vvqwyrvlwr svgsqsinrn etlkppfrfr dmdqgliyef
     1261 lvkagnhygm sastlpvvih ppgsaalagh glslggkiai vlvmtalvlv iaavlgifyw
     1321 krwkakarpp gvsfenptyl kdgvvlqnts gegtakpget tvengghrng rpravencye
     1381 diravrlss
//
LOCUS       XP_077504734             723 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114708 [Amblyomma americanum].
ACCESSION   XP_077504734
VERSION     XP_077504734.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648608.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..723
                     /product="uncharacterized protein LOC144114708"
                     /calculated_mol_wt=77827
     Region          34..74
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(38,41,53,55,58,61,72)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          484..>542
                     /region_name="DLP_helical"
                     /note="Dynamin-like helical domain; pfam18709"
                     /db_xref="CDD:408486"
     Region          582..719
                     /region_name="MATH"
                     /note="MATH (meprin and TRAF-C homology) domain; an
                     independent folding unit with an eight-stranded
                     beta-sandwich structure found in meprins, TRAFs and other
                     proteins. Meprins comprise a class of extracellular
                     metalloproteases which are anchored to the membrane...;
                     cl02446"
                     /db_xref="CDD:445786"
     Site            order(636,701..703)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:238068"
     CDS             1..723
                     /gene="LOC144114708"
                     /coded_by="XM_077648608.1:101..2272"
                     /db_xref="GeneID:144114708"
ORIGIN      
        1 masateksat tytlygfdnd ldwretcfle plppsrycay cgrvcasmmm lpcchiicag
       61 cyaqcatrsn tcaldalsfr qqdvaimpft ipdlaarhik cwnaergcvl sgpsflileh
      121 fhkdclyhsv scsrcgqait hgdviahlss gcsperpqqp sflqnnpral lggedtirql
      181 epenpaasqp lsvdllladd qhpkleredv apslkasalt gryhstnilp ithpiadaar
      241 gpkphsahgg kqapavdsyt pasvarylye qlpsaahtgl lgipsfvdrk idlltgsevp
      301 svsldaevpe nllclsptvv pnpdppqegs vqgrgvppds gsaspetapp grdqyphvaa
      361 iltsgtlsms epnssrgarp ktgttstaeg ktapiqdtgk teendssedq ptlvegsalp
      421 hdkgkselsp egptggsgre aealvtvsgt evpsapgsvg gealssllqg ylneigrlvr
      481 mivagidvvr dalgklmpee lvfvlkacee vraqlkgett tssdwtdeik sgtkhvlqav
      541 qnegdaddal sqeyvaekig hlislgkhvl rqrvrvsvpt qwylegwskl kyravtvgea
      601 sdffhdistp ffgymivpgl mlehtagtlr lrfsfyvrrg iydnflewpl dkkltlsvih
      661 htikgkarsl tvdtkrdlke kclkpgssei akpsvssksl kagcfdtdgy vsgdklllkf
      721 dak
//
LOCUS       XP_077504735             344 aa            linear   INV 25-NOV-2025
DEFINITION  tetraspanin 26A isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504735
VERSION     XP_077504735.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648609.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..344
                     /product="tetraspanin 26A isoform X1"
                     /calculated_mol_wt=38690
     Region          48..>188
                     /region_name="Tetraspanin"
                     /note="Tetraspanin family; pfam00335"
                     /db_xref="CDD:459767"
     Region          145..268
                     /region_name="TM4SF9_like_LEL"
                     /note="Tetraspanin, extracellular domain or large
                     extracellular loop (LEL), TM4SF9_like subfamily.
                     Tetraspanins are trans-membrane proteins with 4
                     trans-membrane segments. Both the N- and C-termini lie on
                     the intracellular side of the membrane. This alignment...;
                     cd03159"
                     /db_xref="CDD:239412"
     Site            order(146,151,155,157..158,161,175,178..179,182..183)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239412"
     CDS             1..344
                     /gene="Tsp26A"
                     /coded_by="XM_077648609.1:16..1050"
                     /db_xref="GeneID:144114709"
ORIGIN      
        1 mtreragkst geklvgkmap ygeeverygn rmpvrkhrrd kseiscclky lifgfnvifw
       61 lmglsimvvg vwawtekdif nnlsrltnia ldpafvlivi ggitfiigft gcvgalrent
      121 cllaayaifl aillllemta gilgfifkdw iksqatngfq afivfyrddp drqnlidwiq
      181 eqwlgccgie gpkdwdmniy fncssvevgs reacgvpfsc ckrqpnelik nkqcgydvrk
      241 aeyqrdkshv iyekgclrag eewieanlvp vagvavglav lqdksqrsrl iyergclqvg
      301 eewlevnlvp lsgallivaf aqilgicfaq nlradifaqk akwh
//
LOCUS       XP_077504736             304 aa            linear   INV 25-NOV-2025
DEFINITION  tetraspanin 26A isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504736
VERSION     XP_077504736.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648610.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..304
                     /product="tetraspanin 26A isoform X2"
                     /calculated_mol_wt=34206
     Region          48..292
                     /region_name="Tetraspanin"
                     /note="Tetraspanin family; pfam00335"
                     /db_xref="CDD:459767"
     Region          145..268
                     /region_name="tetraspanin_LEL"
                     /note="Tetraspanin, extracellular domain or large
                     extracellular loop (LEL). Tetraspanins are trans-membrane
                     proteins with 4 trans-membrane segments. Both the N- and
                     C-termini lie on the intracellular side of the membrane.
                     This alignment model spans the...; cl02781"
                     /db_xref="CDD:351888"
     Site            order(146,151,155,157..158,161,175,178..179,182..183)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239401"
     CDS             1..304
                     /gene="Tsp26A"
                     /coded_by="XM_077648610.1:12..926"
                     /db_xref="GeneID:144114709"
ORIGIN      
        1 mtreragkst geklvgkmap ygeeverygn rmpvrkhrrd kseiscclky lifgfnvifw
       61 lmglsimvvg vwawtekdif nnlsrltnia ldpafvlivi ggitfiigft gcvgalrent
      121 cllaayaifl aillllemta gilgfifkdw iksqatngfq afivfyrddp drqnlidwiq
      181 eqwlgccgie gpkdwdmniy fncssvevgs reacgvpfsc ckrqpnelik nkqcgydvrk
      241 aeyqrdkshv iyekgclrag eewieanlvp vagvavglav lqilgicfaq nlradifaqk
      301 akwh
//
LOCUS       XP_077504737             187 aa            linear   INV 25-NOV-2025
DEFINITION  nudC domain-containing protein 2-like [Amblyomma americanum].
ACCESSION   XP_077504737
VERSION     XP_077504737.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648611.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="nudC domain-containing protein 2-like"
                     /calculated_mol_wt=20682
     Region          13..106
                     /region_name="alpha-crystallin domain (ACD) found in
                     alpha-crystallin-type small heat shock proteins, and a
                     similar domain found in p23 (a cochaperone for Hsp90) and
                     in other p23-like proteins."
                     /note="The alpha-crystallin-Hsps_p23-like superfamily
                     includes the alpha-crystallin domain (ACD) of
                     alpha-crystallin-type small heat shock proteins (sHsps)
                     and a similar domain found in p23-like proteins. sHsps are
                     small stress induced proteins with monomeric...; cl00175"
                     /db_xref="CDD:469641"
     CDS             1..187
                     /gene="LOC144114710"
                     /coded_by="XM_077648611.1:168..731"
                     /db_xref="GeneID:144114710"
ORIGIN      
        1 mplshfders gavscptpwg rwwqtigevf vevevpkgtr gkdvriqitp rhmsctvhgk
       61 qlfsgdlhrt vvadestwti eeqqrvlill vktepassek vweslldgqy kpdplvmhem
      121 mkkldlekfq ienpgfdfsg akldkaydhi pglgggtses qptgfhveqs egdaaarlas
      181 kgsgfsf
//
LOCUS       XP_077504738             187 aa            linear   INV 25-NOV-2025
DEFINITION  nudC domain-containing protein 2-like [Amblyomma americanum].
ACCESSION   XP_077504738
VERSION     XP_077504738.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648612.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="nudC domain-containing protein 2-like"
                     /calculated_mol_wt=20682
     Region          13..106
                     /region_name="alpha-crystallin domain (ACD) found in
                     alpha-crystallin-type small heat shock proteins, and a
                     similar domain found in p23 (a cochaperone for Hsp90) and
                     in other p23-like proteins."
                     /note="The alpha-crystallin-Hsps_p23-like superfamily
                     includes the alpha-crystallin domain (ACD) of
                     alpha-crystallin-type small heat shock proteins (sHsps)
                     and a similar domain found in p23-like proteins. sHsps are
                     small stress induced proteins with monomeric...; cl00175"
                     /db_xref="CDD:469641"
     CDS             1..187
                     /gene="LOC144114710"
                     /coded_by="XM_077648612.1:164..727"
                     /db_xref="GeneID:144114710"
ORIGIN      
        1 mplshfders gavscptpwg rwwqtigevf vevevpkgtr gkdvriqitp rhmsctvhgk
       61 qlfsgdlhrt vvadestwti eeqqrvlill vktepassek vweslldgqy kpdplvmhem
      121 mkkldlekfq ienpgfdfsg akldkaydhi pglgggtses qptgfhveqs egdaaarlas
      181 kgsgfsf
//
LOCUS       XP_077504739             162 aa            linear   INV 25-NOV-2025
DEFINITION  glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial
            [Amblyomma americanum].
ACCESSION   XP_077504739
VERSION     XP_077504739.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648613.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..162
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..162
                     /product="glutamyl-tRNA(Gln) amidotransferase subunit C,
                     mitochondrial"
                     /calculated_mol_wt=17287
     Region          53..138
                     /region_name="gatC"
                     /note="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC; cl00495"
                     /db_xref="CDD:469792"
     CDS             1..162
                     /gene="GatC"
                     /coded_by="XM_077648613.1:262..750"
                     /db_xref="GeneID:144114712"
ORIGIN      
        1 mlrmsalrlc avfsirqsgh irtrelcgst gggsvippep lpkaaraset itldqgtvdl
       61 lerlslvdfs naeavtrlee avkfasvimn vdttgvapmv tpledvplrl rpdiptecsa
      121 edilknakvt eegyfvappg nipldvksdy gltescetsa ek
//
LOCUS       XP_077504740             465 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114713 [Amblyomma americanum].
ACCESSION   XP_077504740
VERSION     XP_077504740.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648614.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..465
                     /product="uncharacterized protein LOC144114713"
                     /calculated_mol_wt=49368
     Region          175..241
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cd00590"
                     /db_xref="CDD:409669"
     Region          258..312
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..465
                     /gene="LOC144114713"
                     /coded_by="XM_077648614.1:174..1571"
                     /db_xref="GeneID:144114713"
ORIGIN      
        1 mafkkgpaqa hkpgskggpp agkkapppkk pqaakadede ddddledddd fsmdedsdls
       61 gadedlddee lddeelddee ldededldee sededvsagk avkpgvaavk agaqqqkgln
      121 kqaqaprqkv pvevtngkrk aedqeivsps kkirleehip asfvvdyesr dlcdvklssf
      181 gdgvttgdvk alckdavevr ekfrgrklvc afvrfaskek atsavqalqg aklkgspvta
      241 sycgerwtnp alrpmylcdt ldvrrvpkqy qnrkklaslf ptgnvvkafp dgyaqvkfps
      301 sdalikalkn pkcrtidgen lqfslavlhk dkpanlkarg saatkltgdk adskgqrkka
      361 kkaakqkaga espvkgsspg kkpgtpahqg spktpgqqqq kgpktpgqqk gvktpgkegg
      421 pktpgqpkgg aktpgeapkg tpkgtpkatp sqkktpglkg tpkva
//
LOCUS       XP_077504741             363 aa            linear   INV 25-NOV-2025
DEFINITION  protein phosphatase Mn(2+)-dependent 1K-like [Amblyomma
            americanum].
ACCESSION   XP_077504741
VERSION     XP_077504741.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648615.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..363
                     /product="protein phosphatase Mn(2+)-dependent 1K-like"
                     /calculated_mol_wt=40106
     Region          83..337
                     /region_name="PP2Cc"
                     /note="Serine/threonine phosphatases, family 2C, catalytic
                     domain; The protein architecture and deduced catalytic
                     mechanism of PP2C phosphatases are similar to the PP1,
                     PP2A, PP2B family of protein Ser/Thr phosphatases, with
                     which PP2C shares no sequence...; cd00143"
                     /db_xref="CDD:238083"
     Site            order(93,97..98,116..118,289,328)
                     /site_type="active"
                     /db_xref="CDD:238083"
     CDS             1..363
                     /gene="LOC144114714"
                     /coded_by="XM_077648615.1:221..1312"
                     /db_xref="GeneID:144114714"
ORIGIN      
        1 msllllrlnl prvsrlnrdv lkplfrpsvr ekqvlsdpen igqrqqhinf dtlgtwdnri
       61 dlplllqqsi kvgkpvpkis gehagcatil grrtynedry rvkeltpdll yfavfdghgg
      121 stcadycaeh meahimywvr rgqtdlqall qsafidinns fakyvafnwp dgeetssgtt
      181 atvcllrsst elyvahvgds rallcrsges rrlttdhtag lkleeerikm sggklisdsh
      241 grhlvngrla mtrslgdldl kpygvvalpd vrsmevkhgk dafvilttdg vncamsdqei
      301 vdainscssp aeaagfvtdq amhfasqdna tavvmpfgaw gkyrnsggva ssfgrqlqfs
      361 rrf
//
LOCUS       XP_077504743             221 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114716 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504743
VERSION     XP_077504743.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648617.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..221
                     /product="uncharacterized protein LOC144114716 isoform X1"
                     /calculated_mol_wt=24246
     CDS             1..221
                     /gene="LOC144114716"
                     /coded_by="XM_077648617.1:5..670"
                     /db_xref="GeneID:144114716"
ORIGIN      
        1 magrrigrav vlrahlpvet fsdssrrwar pqqvphitnv rcsatvrapv rywqifgtpp
       61 mterwcqsrp cpnpshgyll lpryhphpgp gasesstcrd rrpcapdspr pavphpagpe
      121 ptspltpaps palttrhvdm ttpwrpetpl tredtaplpm plgtskgfgr rllatflveg
      181 lqqptlhgaa fsgprhlfsi patwpkivpt iqhlglqaes h
//
LOCUS       XP_077504744             195 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114716 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504744
VERSION     XP_077504744.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648618.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..195
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..195
                     /product="uncharacterized protein LOC144114716 isoform X2"
                     /calculated_mol_wt=21433
     CDS             1..195
                     /gene="LOC144114716"
                     /coded_by="XM_077648618.1:5..592"
                     /db_xref="GeneID:144114716"
ORIGIN      
        1 magrrigrav vlrahlpvet fsdssrrwar pqqvphitnv rcsatvrapv rywqifgtpp
       61 mterwcqsrp cpnpshgyll lpryhphpgp gasesstcrd rrpcapdspr pavphpagpe
      121 ptspltpaps palttrhvdm ttpwrpetpl tleglqqptl hgaafsgprh lfsipatwpk
      181 ivptiqhlgl qaesh
//
LOCUS       XP_077504745             180 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114716 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504745
VERSION     XP_077504745.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648619.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..180
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..180
                     /product="uncharacterized protein LOC144114716 isoform X3"
                     /calculated_mol_wt=19797
     CDS             1..180
                     /gene="LOC144114716"
                     /coded_by="XM_077648619.1:5..547"
                     /db_xref="GeneID:144114716"
ORIGIN      
        1 magrrigrav vlrahlpvet fsdssrrwar pqqvphitnv rcsatvrapv rywqifgtpp
       61 mterwcqsrp cpnpshgyll lpryhphpgp gasesstcrd rrpcapdspr pavphpagpe
      121 ptspltpaps palttrhvdm ttpwrpetpl tredtaplpm plgtskgfgr rllatflgpf
//
LOCUS       XP_077504746             154 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114716 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077504746
VERSION     XP_077504746.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648620.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..154
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..154
                     /product="uncharacterized protein LOC144114716 isoform X4"
                     /calculated_mol_wt=17069
     CDS             1..154
                     /gene="LOC144114716"
                     /coded_by="XM_077648620.1:5..469"
                     /db_xref="GeneID:144114716"
ORIGIN      
        1 magrrigrav vlrahlpvet fsdssrrwar pqqvphitnv rcsatvrapv rywqifgtpp
       61 mterwcqsrp cpnpshgyll lpryhphpgp gasesstcrd rrpcapdspr pavphpagpe
      121 ptspltpaps palttrhvdm ttpwrpetpl trpf
//
LOCUS       XP_077504747             221 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114718 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504747
VERSION     XP_077504747.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648621.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..221
                     /product="uncharacterized protein LOC144114718 isoform X1"
                     /calculated_mol_wt=24246
     CDS             1..221
                     /gene="LOC144114718"
                     /coded_by="XM_077648621.1:5..670"
                     /db_xref="GeneID:144114718"
ORIGIN      
        1 magrrigrav vlrahlpvet fsdssrrwar pqqvphitnv rcsatvrapv rywqifgtpp
       61 mterwcqsrp cpnpshgyll lpryhphpgp gasesstcrd rrpcapdspr pavphpagpe
      121 ptspltpaps palttrhvdm ttpwrpetpl tredtaplpm plgtskgfgr rllatflveg
      181 lqqptlhgaa fsgprhlfsi patwpkivpt iqhlglqaes h
//
LOCUS       XP_077504748             195 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114718 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504748
VERSION     XP_077504748.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648622.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..195
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..195
                     /product="uncharacterized protein LOC144114718 isoform X2"
                     /calculated_mol_wt=21433
     CDS             1..195
                     /gene="LOC144114718"
                     /coded_by="XM_077648622.1:5..592"
                     /db_xref="GeneID:144114718"
ORIGIN      
        1 magrrigrav vlrahlpvet fsdssrrwar pqqvphitnv rcsatvrapv rywqifgtpp
       61 mterwcqsrp cpnpshgyll lpryhphpgp gasesstcrd rrpcapdspr pavphpagpe
      121 ptspltpaps palttrhvdm ttpwrpetpl tleglqqptl hgaafsgprh lfsipatwpk
      181 ivptiqhlgl qaesh
//
LOCUS       XP_077504749             180 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114718 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504749
VERSION     XP_077504749.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648623.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..180
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..180
                     /product="uncharacterized protein LOC144114718 isoform X3"
                     /calculated_mol_wt=19797
     CDS             1..180
                     /gene="LOC144114718"
                     /coded_by="XM_077648623.1:5..547"
                     /db_xref="GeneID:144114718"
ORIGIN      
        1 magrrigrav vlrahlpvet fsdssrrwar pqqvphitnv rcsatvrapv rywqifgtpp
       61 mterwcqsrp cpnpshgyll lpryhphpgp gasesstcrd rrpcapdspr pavphpagpe
      121 ptspltpaps palttrhvdm ttpwrpetpl tredtaplpm plgtskgfgr rllatflgpf
//
LOCUS       XP_077504750             154 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114718 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077504750
VERSION     XP_077504750.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648624.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..154
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..154
                     /product="uncharacterized protein LOC144114718 isoform X4"
                     /calculated_mol_wt=17069
     CDS             1..154
                     /gene="LOC144114718"
                     /coded_by="XM_077648624.1:5..469"
                     /db_xref="GeneID:144114718"
ORIGIN      
        1 magrrigrav vlrahlpvet fsdssrrwar pqqvphitnv rcsatvrapv rywqifgtpp
       61 mterwcqsrp cpnpshgyll lpryhphpgp gasesstcrd rrpcapdspr pavphpagpe
      121 ptspltpaps palttrhvdm ttpwrpetpl trpf
//
LOCUS       XP_077504752             453 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114725 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504752
VERSION     XP_077504752.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648626.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..453
                     /product="uncharacterized protein LOC144114725 isoform X1"
                     /calculated_mol_wt=47882
     CDS             1..453
                     /gene="LOC144114725"
                     /coded_by="XM_077648626.1:105..1466"
                     /db_xref="GeneID:144114725"
ORIGIN      
        1 mppkktaaka qpapakasak kpvkkvvpve edededdkme ddedgedada gmdeavneee
       61 eeaaaneeee edeeeddeea apppakskak nglqvkadva qeeedeeeev eevdaeeeee
      121 eeeeeeqeee deeeeqgdtp kkskkaakdk kadgatksgk rkaaddehsa ggsdakklkl
      181 edyvpescid sqekrdscsl vitmargarp gditelsgdi inlvvkgera faryvdaeta
      241 aenlklleke agkgkvarve kctthdtetq dnlldvfrvp fectvsrlke lfpgakvyvl
      301 ndgfarlhfd ssedllnavq npechtidgs sirfslvkkf ggqgggfrgg sqsrgqsrgg
      361 rggggnnsfs rgggggrggg swgnsnwggg rggwgnsggs wgrggggrgg gkfgggrgdf
      421 ggrggrgggg rfggrggggr grggrggrfg fyy
//
LOCUS       XP_077504753             453 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114725 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504753
VERSION     XP_077504753.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648627.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..453
                     /product="uncharacterized protein LOC144114725 isoform X2"
                     /calculated_mol_wt=47709
     CDS             1..453
                     /gene="LOC144114725"
                     /coded_by="XM_077648627.1:105..1466"
                     /db_xref="GeneID:144114725"
ORIGIN      
        1 mppkktaaka qpapakasak kpvkkvvpve edededdkme ddedgedada gmdeavneee
       61 eeaaaneeee edeeeddeea apppakskak nglqvkadva qeeedeeeev eevdaeeeee
      121 eeeeeeqeee deeeeqgdtp kkskkaakdk kadgatksgk rkaaddehsa ggsdakklkl
      181 edyvpescid sqekrdscsl vitmargarp gditelsgdi inlvvkgera faryvdaeta
      241 aenlklleke agkgkvarve kctthdtetq dnlldvfrvp fectvsrlke lfpgakvyvl
      301 ndgfarlhfd ssedllnavq npechtidgs sirfslvkkf ggqgggfrgg sqsrgqsrgg
      361 rggggnnsfs rgggggrggg swgnsnwggg rggwgnsggs wgrggggrgg gkfgggrgdf
      421 ggrggrgggg rfggrggggr grggrggrfg ged
//
LOCUS       XP_077504754             453 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114725 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504754
VERSION     XP_077504754.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648628.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..453
                     /product="uncharacterized protein LOC144114725 isoform X1"
                     /calculated_mol_wt=47882
     CDS             1..453
                     /gene="LOC144114725"
                     /coded_by="XM_077648628.1:105..1466"
                     /db_xref="GeneID:144114725"
ORIGIN      
        1 mppkktaaka qpapakasak kpvkkvvpve edededdkme ddedgedada gmdeavneee
       61 eeaaaneeee edeeeddeea apppakskak nglqvkadva qeeedeeeev eevdaeeeee
      121 eeeeeeqeee deeeeqgdtp kkskkaakdk kadgatksgk rkaaddehsa ggsdakklkl
      181 edyvpescid sqekrdscsl vitmargarp gditelsgdi inlvvkgera faryvdaeta
      241 aenlklleke agkgkvarve kctthdtetq dnlldvfrvp fectvsrlke lfpgakvyvl
      301 ndgfarlhfd ssedllnavq npechtidgs sirfslvkkf ggqgggfrgg sqsrgqsrgg
      361 rggggnnsfs rgggggrggg swgnsnwggg rggwgnsggs wgrggggrgg gkfgggrgdf
      421 ggrggrgggg rfggrggggr grggrggrfg fyy
//
LOCUS       XP_077504756             533 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114726 [Amblyomma americanum].
ACCESSION   XP_077504756
VERSION     XP_077504756.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648630.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..533
                     /product="uncharacterized protein LOC144114726"
                     /calculated_mol_wt=59904
     Region          <173..200
                     /region_name="zf_PR_Knuckle"
                     /note="PR zinc knuckle motif; pfam18445"
                     /db_xref="CDD:375871"
     Region          209..333
                     /region_name="PR-SET_PRDM7_9"
                     /note="PR-SET domain found in PR domain zinc finger
                     protein 7 (PRDM7) and 9 (PRDM9) and similar proteins;
                     cd19193"
                     /db_xref="CDD:380970"
     Site            order(223,226..228,245,255..256,258..262,271,284..288,290,
                     299..300,324..325,327..328,331..332)
                     /site_type="active"
                     /db_xref="CDD:380970"
     Site            order(223,226..228,258..259,284..288,290,324,328,332)
                     /site_type="other"
                     /note="SAM binding site"
                     /db_xref="CDD:380970"
     Region          361..380
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(361,364,377,380)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          392..412
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(392,395,408,412)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          404..429
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          420..440
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(420,423,436,440)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(425,427,429,431..432,435..436,439,485,487,491..492,
                     495..496,499,513,515,517,519..520,523..524)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          <445..>511
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          448..500
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          508..526
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..533
                     /gene="LOC144114726"
                     /coded_by="XM_077648630.1:1835..3436"
                     /db_xref="GeneID:144114726"
ORIGIN      
        1 maseddftef rkhftdeewk glweymrtap snikekyelm lklglkpprh efmkpkpapp
       61 ipakpptksn spspeasssg sgyprhqree akcmeceens dqeylsspet sssgsgyprh
      121 qrkeakrmec eensdqeyls spetsssgsg yprhqrkeak rmeceensdq eylfcddcgv
      181 drpgdcpkhg plthvkdsev dagdplrank tlpeglsirr stikgaqhgv ftlkplpkrv
      241 yfgpyegvkv edngggngyt wqvfedgkvf lvdgrpldss nwmryvncaa spqeqnlvaf
      301 trhgniyyrt pkavgageel lvwygaafar elgllgkprg sgpsakedgd searpqpvfs
      361 cdtcgepfyt pdclekhrrh nhlqrperrh rcahcpyssd rkhnvvther thtgerpflc
      421 evcgkdfarq hhlkghqrvh sgegphecpe cgqrfsqasn lathrsgqhg vdggaashlc
      481 prcgkgfsqk ghlnrhqlth tgerphacsl cakrfarhde avrheravhg hqh
//
LOCUS       XP_077504757             474 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114727 [Amblyomma americanum].
ACCESSION   XP_077504757
VERSION     XP_077504757.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648631.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..474
                     /product="uncharacterized protein LOC144114727"
                     /calculated_mol_wt=52976
     Region          <114..141
                     /region_name="zf_PR_Knuckle"
                     /note="PR zinc knuckle motif; pfam18445"
                     /db_xref="CDD:375871"
     Region          150..273
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(168..169,224..229,240..242,263..266)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Site            order(168..169,225..229,265)
                     /site_type="other"
                     /note="SAM binding site [polypeptide binding]"
                     /db_xref="CDD:380914"
     Region          <292..465
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          302..322
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(302,305,318,322)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          333..353
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(333,336,349,353)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(338,340,342,344..345,348..349,352,366,368,372..373,
                     376..377,380,394,396,398,400..401,404..405)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          361..381
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          389..406
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          449..468
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..474
                     /gene="LOC144114727"
                     /coded_by="XM_077648631.1:169..1593"
                     /db_xref="GeneID:144114727"
ORIGIN      
        1 meceensdqe ylsspetsss gsgyprhqrk eakrmeceqn sdqeylsspe tsssgsgypr
       61 hqrkeakrme ceensdqeyl sspetsssgs gyprhqrkea krmeceensd qeylfcddcg
      121 vdrpgdcpkh gplthvkdse vdagdplran ktlpeglsir hstikgaqhg vftlkplpkr
      181 vyfgpyegvk vedngggngy twqvfedgkv flvdgrplds snwmryvnca aspqeqnlva
      241 ftrhgniyyr tpkavgagee llvwygaefa relellgkpr gsgpsakedg dsearpqpvf
      301 scdtcgeafy tpdclekhrr hnhpqrpegr hrcahcpyss drkhnvvthe rthtgerpfl
      361 cevcgkgfsq lqhltahqri hsgegphecp ecgqrfsqaa nlashrsgqh gvdggaashl
      421 cprcgkgftr egnlnrhqlt htgerphacs lcakrfarld vakkhervvh grqy
//
LOCUS       XP_077504758             464 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114728 [Amblyomma americanum].
ACCESSION   XP_077504758
VERSION     XP_077504758.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648632.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..464
                     /product="uncharacterized protein LOC144114728"
                     /calculated_mol_wt=51588
     Region          <104..131
                     /region_name="zf_PR_Knuckle"
                     /note="PR zinc knuckle motif; pfam18445"
                     /db_xref="CDD:375871"
     Region          140..264
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(158..159,214..219,230..232,253..256)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Site            order(158..159,215..219,255)
                     /site_type="other"
                     /note="SAM binding site [polypeptide binding]"
                     /db_xref="CDD:380914"
     Region          <282..455
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          292..312
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(292,295,308,312)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          323..343
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(323,326,339,343)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(328,330,332,334..335,338..339,342,356,358,362..363,
                     366..367,370,384,386,388,390..391,394..395)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          351..371
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          379..396
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          439..457
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..464
                     /gene="LOC144114728"
                     /coded_by="XM_077648632.1:3721..5115"
                     /db_xref="GeneID:144114728"
ORIGIN      
        1 miltciftgl kpplhefmkp kpapaipakp ptksnspspe tsssgsrypr rqrkeakcme
       61 ceensdqeyl sspepsssgs cyprrqrkea kcmeceensd qeylfcddcg vdrpgdcpkh
      121 gplthvkdae vdagdplran ktlpeglsir rstikgaqyg vftlkslpkr vyfgpyegvk
      181 vedngggngy twqvfedgkv flvdgrpldr snwmryvnra aspqeqnlva ftrhgniyyr
      241 tpkavgagee llvwygaafa relgllgkpr gagpsakedg dsearpqpvf scdtcgepfy
      301 tpdclekhrr hnhpqrpegr hrcahcpyss drkhnvvthe rthtgerpfl chvcgkgfsq
      361 lqhltahqri hsgegphecp ecgqrfsqaa nlashrsgqh gvdggaashl cprcgkgftr
      421 kgnlnrhqlt htgerphacs lcaerfarld vakkheravh grqy
//
LOCUS       XP_077504759             904 aa            linear   INV 25-NOV-2025
DEFINITION  general vesicular transport factor p115 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504759
VERSION     XP_077504759.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648633.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..904
                     /product="general vesicular transport factor p115 isoform
                     X1"
                     /calculated_mol_wt=99900
     Region          90..115
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          127..160
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          170..207
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          288..346
                     /region_name="Arm_vescicular"
                     /note="Armadillo tether-repeat of vescicular transport
                     factor; pfam18770"
                     /db_xref="CDD:465861"
     Region          364..644
                     /region_name="Uso1_p115_head"
                     /note="Uso1 / p115 like vesicle tethering protein, head
                     region; pfam04869"
                     /db_xref="CDD:461460"
     Region          <674..820
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          795..883
                     /region_name="Uso1_p115_C"
                     /note="Uso1 / p115 like vesicle tethering protein, C
                     terminal region; pfam04871"
                     /db_xref="CDD:461461"
     CDS             1..904
                     /gene="p115"
                     /coded_by="XM_077648633.1:69..2783"
                     /db_xref="GeneID:144114730"
ORIGIN      
        1 melfksglkt vlggqpgaha ptgaetverl vnrvqtstll edrrdacral kalakkfrve
       61 vgaqgmdali hiletdrgdg eiigyaldal slvisgaeen eedsqdenag relgeqftei
      121 yikkpenvtl lldlmeefdf rvrwpavrll tgllvhrake vqecvltspm gvsrlmdlls
      181 dsrevirnda llllshltka naniqkivaf enafdrlldi iidegcsdgg vvvedclvvl
      241 lhllknnnsn qnffkegsyi qrlspffnyh htqqqqegss daqqsgggws pqkvsnifhm
      301 lqvvrslvsp saplnvtssc qkamnqsgll eqlcnilmav gvpadiltet intvaevirg
      361 hhsnqeyfas vnapsnpprp aivvllmsmv nekqpfvlrc avlycfqcfl yknelgqaqi
      421 iqtllptcad mtsvtagqll cgglfstdpl snwfaavals halvdnptqk eqllrvqlat
      481 svgnppvslm rqctgilqqg gklqtrlgll mlmstwlanc tlavtsflni ptnipyltsq
      541 vglaegdehe dlvqglcafl lgiciefndd svpsftresl cqllmkrvgl dtfvdklvai
      601 skqecysqaa qkpqlkykhp sevffdyefc rlfkslegti ikavqprpkd lqngpesnmt
      661 aeqhslllqy kgvirdqder iksmtselet lrrehqestc elkemmetmq qlkdqnallk
      721 aqrvaagggq gdskspspel dqtkreledl rkqheqvlqe metlkaerms qafaaeaasa
      781 pssevvttpa vdpvlkaeld sakeriaale aevsslnqvn tvlkkekeas eeelqmlrke
      841 qedllvmlte qdtrisklka kfkelgveae pdvvpdlges dddlgsdlge tedlvvvgep
      901 neev
//
LOCUS       XP_077504760             899 aa            linear   INV 25-NOV-2025
DEFINITION  general vesicular transport factor p115 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504760
VERSION     XP_077504760.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648634.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..899
                     /product="general vesicular transport factor p115 isoform
                     X2"
                     /calculated_mol_wt=99389
     Region          90..115
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          127..160
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          170..207
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          288..346
                     /region_name="Arm_vescicular"
                     /note="Armadillo tether-repeat of vescicular transport
                     factor; pfam18770"
                     /db_xref="CDD:465861"
     Region          364..644
                     /region_name="Uso1_p115_head"
                     /note="Uso1 / p115 like vesicle tethering protein, head
                     region; pfam04869"
                     /db_xref="CDD:461460"
     Region          <674..820
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          795..885
                     /region_name="Uso1_p115_C"
                     /note="Uso1 / p115 like vesicle tethering protein, C
                     terminal region; pfam04871"
                     /db_xref="CDD:461461"
     CDS             1..899
                     /gene="p115"
                     /coded_by="XM_077648634.1:69..2768"
                     /db_xref="GeneID:144114730"
ORIGIN      
        1 melfksglkt vlggqpgaha ptgaetverl vnrvqtstll edrrdacral kalakkfrve
       61 vgaqgmdali hiletdrgdg eiigyaldal slvisgaeen eedsqdenag relgeqftei
      121 yikkpenvtl lldlmeefdf rvrwpavrll tgllvhrake vqecvltspm gvsrlmdlls
      181 dsrevirnda llllshltka naniqkivaf enafdrlldi iidegcsdgg vvvedclvvl
      241 lhllknnnsn qnffkegsyi qrlspffnyh htqqqqegss daqqsgggws pqkvsnifhm
      301 lqvvrslvsp saplnvtssc qkamnqsgll eqlcnilmav gvpadiltet intvaevirg
      361 hhsnqeyfas vnapsnpprp aivvllmsmv nekqpfvlrc avlycfqcfl yknelgqaqi
      421 iqtllptcad mtsvtagqll cgglfstdpl snwfaavals halvdnptqk eqllrvqlat
      481 svgnppvslm rqctgilqqg gklqtrlgll mlmstwlanc tlavtsflni ptnipyltsq
      541 vglaegdehe dlvqglcafl lgiciefndd svpsftresl cqllmkrvgl dtfvdklvai
      601 skqecysqaa qkpqlkykhp sevffdyefc rlfkslegti ikavqprpkd lqngpesnmt
      661 aeqhslllqy kgvirdqder iksmtselet lrrehqestc elkemmetmq qlkdqnallk
      721 aqrvaagggq gdskspspel dqtkreledl rkqheqvlqe metlkaerms qafaaeaasa
      781 pssevvttpa vdpvlkaeld sakeriaale aevsslnqvn tvlkkekeas eeelqmlrke
      841 qedllvmlte qdtrisklka kfkelgvevp dlgesdddlg sdlgetedlv vvgepneev
//
LOCUS       XP_077504761             316 aa            linear   INV 25-NOV-2025
DEFINITION  palmitoyl-protein thioesterase 1-like [Amblyomma americanum].
ACCESSION   XP_077504761
VERSION     XP_077504761.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648635.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..316
                     /product="palmitoyl-protein thioesterase 1-like"
                     /calculated_mol_wt=35329
     Region          43..298
                     /region_name="Palm_thioest"
                     /note="Palmitoyl protein thioesterase; pfam02089"
                     /db_xref="CDD:460441"
     CDS             1..316
                     /gene="LOC144114731"
                     /coded_by="XM_077648635.1:80..1030"
                     /db_xref="GeneID:144114731"
ORIGIN      
        1 mgfcafswav lsvclsvals sadfsdgivy psahdgsgsq afpivmwhgl gdsccnplsl
       61 grfkkflees ipgvyvhslq igsnmideig dsyfmntneq vakaceiian dtklsggyna
      121 igfsqggqfm ravaqrcpep plrnlislgg qhqgvfglpr cpgessslce yarkllniga
      181 ywdvvqdhlv paqywhdpfn hktytakslf ladinnervk netykanllk lmnlvlvqfq
      241 ndtvvdpkas swfdyyrend dktivplrdq diytedwigl kqldqsgrlh flstlgdhlq
      301 fttewfteni lkpyll
//
LOCUS       XP_077504762             908 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114733 [Amblyomma americanum].
ACCESSION   XP_077504762
VERSION     XP_077504762.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648636.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..908
                     /product="uncharacterized protein LOC144114733"
                     /calculated_mol_wt=103929
     Region          <265..308
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cl21498"
                     /db_xref="CDD:473887"
     Region          299..340
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cd00167"
                     /db_xref="CDD:238096"
     Region          353..416
                     /region_name="Myb_DNA-bind_6"
                     /note="Myb-like DNA-binding domain; pfam13921"
                     /db_xref="CDD:372817"
     Site            order(353,386..387,389..390,392..394,396..397)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238096"
     Region          405..449
                     /region_name="Myb_DNA-binding"
                     /note="Myb-like DNA-binding domain; pfam00249"
                     /db_xref="CDD:459731"
     Region          458..505
                     /region_name="SANT"
                     /note="SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding
                     domains; smart00717"
                     /db_xref="CDD:197842"
     Site            order(460,490..491,493..494,496..498,500..502)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238096"
     CDS             1..908
                     /gene="LOC144114733"
                     /coded_by="XM_077648636.1:117..2843"
                     /db_xref="GeneID:144114733"
ORIGIN      
        1 mdefdmdyse pgpsnatrld lrpqiafvpg dsaaeefdsd esdeqfyday seyvdvgtpv
       61 maeendsdnd aedesllgss ntealsdlek ilcanhvyqe flenairdft diieknrklq
      121 eelparlaer tqrrskpkvr knwyqalifh hpyfrdvngm pappnedelt krankeldpy
      181 itparawsak efctlvkavq dnllqqnlea lldrkktlse rllttedpsq naelkerisq
      241 ldeqmskvre mtlvelleqs trpidwlria amdmnsdrta fgcemhwrhl ldvrlnqgpw
      301 tpeeeerlct lvtkwdercw dqvaaelntg rsafqcalhy crnhavrvni gpftptedkr
      361 lrllinlcte gdniywsqva hfmgtrskkq vmnryyrsmd philhgrwta eedmmlliai
      421 niygsegwsk vatlmpgrtk hqcrehyiks facdiisgpy tpsedkalve lvdkhglgcw
      481 aqvakgmpwr tsnsvltryt rlsrmvgkdk vtaadlekiy akpdsmvter prmqtpaivr
      541 rmnvfrricr mintstqrev alklvkgdse tvdretclql yrrllhkfqs grwshhaptq
      601 arslnraiaq yaqplyrrpw rnmaayehee wqavtnvlhd vhnlerspgd pkfeqmdtvp
      661 vfeeflctsv lgvpanfqpg tshqlpllap cettmatfgr lvdrfvdgdv ekcwsflqdf
      721 esfpelcaal davnvesirc tdctsraple delssmanre lavhldqlac rrcvlmretr
      781 rnydvlrsrf lsyffwplll dkqnvaetvm lppdtsskgr kvrrvrkkqk kpwvkeawaq
      841 rrrqaaqaaa aaesqggqgd asgeeavaed ndgeddeamp stsqgegsdg nmavdegsgd
      901 ettpstsq
//
LOCUS       XP_077504763             455 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114734 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504763
VERSION     XP_077504763.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648637.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..455
                     /product="uncharacterized protein LOC144114734 isoform X1"
                     /calculated_mol_wt=50479
     Region          21..>211
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     Site            order(23..25,40,63,103..105,112..113)
                     /site_type="other"
                     /note="heterotrimeric interface"
                     /db_xref="CDD:187751"
     CDS             1..455
                     /gene="LOC144114734"
                     /coded_by="XM_077648637.1:93..1460"
                     /db_xref="GeneID:144114734"
ORIGIN      
        1 marqlkneav sseddsepsr lfllpshcat gserrhyifp lprpnteehs mfmatdsgdl
       61 melakyqmeh esftadgpfe talhvfsrhg wqvacyaash ppesfrtsrl pvvhsstlag
      121 rdqpvymatp vdalffalap lykhndrfwp lkdivedcll lkvirscsdr mqhiadvegl
      181 gkdqvycyne skclawltkk vkavaarlqs igipppaacs saasagssgg iqptsedcmv
      241 aahaivaryi ppmlatvlks slgldtrpat tveeekqktp slpqlkkkkf kqvqqqesnn
      301 kgapknsvqk sakkkkskqv qqqennnega pensvqksak kkkskqvqqq esnnkgapen
      361 svqksakkkk skqvqqqesn nkgspknseq ksakkkkskq vqqqesnnkg spknseqksa
      421 karsqeflsg etqdtkddsq teeregsqsw heehf
//
LOCUS       XP_077504765             454 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114734 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504765
VERSION     XP_077504765.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648639.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..454
                     /product="uncharacterized protein LOC144114734 isoform X2"
                     /calculated_mol_wt=50350
     Region          21..>210
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     Site            order(23..25,40,62,102..104,111..112)
                     /site_type="other"
                     /note="heterotrimeric interface"
                     /db_xref="CDD:187751"
     CDS             1..454
                     /gene="LOC144114734"
                     /coded_by="XM_077648639.1:67..1431"
                     /db_xref="GeneID:144114734"
ORIGIN      
        1 marqlkneav sseddsepsr lfllpshcat gserrhyifp lprpntehsm fmatdsgdlm
       61 elakyqmehe sftadgpfet alhvfsrhgw qvacyaashp pesfrtsrlp vvhsstlagr
      121 dqpvymatpv dalffalapl ykhndrfwpl kdivedclll kvirscsdrm qhiadveglg
      181 kdqvycynes kclawltkkv kavaarlqsi gipppaacss aasagssggi qptsedcmva
      241 ahaivaryip pmlatvlkss lgldtrpatt veeekqktps lpqlkkkkfk qvqqqesnnk
      301 gapknsvqks akkkkskqvq qqennnegap ensvqksakk kkskqvqqqe snnkgapens
      361 vqksakkkks kqvqqqesnn kgspknseqk sakkkkskqv qqqesnnkgs pknseqksak
      421 arsqeflsge tqdtkddsqt eeregsqswh eehf
//
LOCUS       XP_077504766             449 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114734 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504766
VERSION     XP_077504766.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648640.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..449
                     /product="uncharacterized protein LOC144114734 isoform X3"
                     /calculated_mol_wt=49637
     Region          21..>211
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     Site            order(23..25,40,63,103..105,112..113)
                     /site_type="other"
                     /note="heterotrimeric interface"
                     /db_xref="CDD:187751"
     CDS             1..449
                     /gene="LOC144114734"
                     /coded_by="XM_077648640.1:93..1442"
                     /db_xref="GeneID:144114734"
ORIGIN      
        1 marqlkneav sseddsepsr lfllpshcat gserrhyifp lprpnteehs mfmatdsgdl
       61 melakyqmeh esftadgpfe talhvfsrhg wqvacyaash ppesfrtsrl pvvhsstlag
      121 rdqpvymatp vdalffalap lykhndrfwp lkdivedcll lkvirscsdr mqhiadvegl
      181 gkdqvycyne skclawltkk vkavaarlqs igipppaacs saasagssgg iqptsedcmv
      241 aahaivaryi ppmlatvlks slgldtrpat tveeekqktp slpqlkkkkf kqvqqqesnn
      301 kgapknsvqk sakkkkskqv qqqennnega pensvqksak kkkskqvqqq esnnkgapen
      361 svqksakkkk skqvqqqesn nkgspknseq ksakkkkskq vqqqesnnkg spknseqksa
      421 ktprttvrqk rekaakagmk sissyfakk
//
LOCUS       XP_077504767             443 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114734 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077504767
VERSION     XP_077504767.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648641.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="uncharacterized protein LOC144114734 isoform X4"
                     /calculated_mol_wt=49260
     Region          25..>199
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     CDS             1..443
                     /gene="LOC144114734"
                     /coded_by="XM_077648641.1:121..1452"
                     /db_xref="GeneID:144114734"
ORIGIN      
        1 mtrsrrdcsc cqvrhcatgs errhyifplp rpnteehsmf matdsgdlme lakyqmehes
       61 ftadgpfeta lhvfsrhgwq vacyaashpp esfrtsrlpv vhsstlagrd qpvymatpvd
      121 alffalaply khndrfwplk divedclllk virscsdrmq hiadveglgk dqvycynesk
      181 clawltkkvk avaarlqsig ipppaacssa asagssggiq ptsedcmvaa haivaryipp
      241 mlatvlkssl gldtrpattv eeekqktpsl pqlkkkkfkq vqqqesnnkg apknsvqksa
      301 kkkkskqvqq qennnegape nsvqksakkk kskqvqqqes nnkgapensv qksakkkksk
      361 qvqqqesnnk gspknseqks akkkkskqvq qqesnnkgsp knseqksaka rsqeflsget
      421 qdtkddsqte eregsqswhe ehf
//
LOCUS       XP_077504768             442 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114734 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077504768
VERSION     XP_077504768.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648642.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..442
                     /product="uncharacterized protein LOC144114734 isoform X5"
                     /calculated_mol_wt=49131
     Region          79..>198
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     CDS             1..442
                     /gene="LOC144114734"
                     /coded_by="XM_077648642.1:107..1435"
                     /db_xref="GeneID:144114734"
ORIGIN      
        1 mtrsrrdcsc cqvrhcatgs errhyifplp rpntehsmfm atdsgdlmel akyqmehesf
       61 tadgpfetal hvfsrhgwqv acyaashppe sfrtsrlpvv hsstlagrdq pvymatpvda
      121 lffalaplyk hndrfwplkd ivedclllkv irscsdrmqh iadveglgkd qvycyneskc
      181 lawltkkvka vaarlqsigi pppaacssaa sagssggiqp tsedcmvaah aivaryippm
      241 latvlksslg ldtrpattve eekqktpslp qlkkkkfkqv qqqesnnkga pknsvqksak
      301 kkkskqvqqq ennnegapen svqksakkkk skqvqqqesn nkgapensvq ksakkkkskq
      361 vqqqesnnkg spknseqksa kkkkskqvqq qesnnkgspk nseqksakar sqeflsgetq
      421 dtkddsqtee regsqswhee hf
//
LOCUS       XP_077504769             431 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114734 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077504769
VERSION     XP_077504769.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648643.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..431
                     /product="uncharacterized protein LOC144114734 isoform X6"
                     /calculated_mol_wt=48005
     Region          68..>187
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     CDS             1..431
                     /gene="LOC144114734"
                     /coded_by="XM_077648643.1:133..1428"
                     /db_xref="GeneID:144114734"
ORIGIN      
        1 mtrsrrdcsc cqaiaplevs egitfshcpv qiqkntrcsw rltrvtswsw rntrwsmnhl
       61 rlmallrqva cyaashppes frtsrlpvvh sstlagrdqp vymatpvdal ffalaplykh
      121 ndrfwplkdi vedclllkvi rscsdrmqhi adveglgkdq vycyneskcl awltkkvkav
      181 aarlqsigip ppaacssaas agssggiqpt sedcmvaaha ivaryippml atvlksslgl
      241 dtrpattvee ekqktpslpq lkkkkfkqvq qqesnnkgap knsvqksakk kkskqvqqqe
      301 nnnegapens vqksakkkks kqvqqqesnn kgapensvqk sakkkkskqv qqqesnnkgs
      361 pknseqksak kkkskqvqqq esnnkgspkn seqksakars qeflsgetqd tkddsqteer
      421 egsqswheeh f
//
LOCUS       XP_077504770             430 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114734 isoform X7 [Amblyomma
            americanum].
ACCESSION   XP_077504770
VERSION     XP_077504770.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648644.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..430
                     /product="uncharacterized protein LOC144114734 isoform X7"
                     /calculated_mol_wt=47876
     Region          67..>186
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     CDS             1..430
                     /gene="LOC144114734"
                     /coded_by="XM_077648644.1:107..1399"
                     /db_xref="GeneID:144114734"
ORIGIN      
        1 mtrsrrdcsc cqaiaplevs egitfshcpv qiqntrcswr ltrvtswswr ntrwsmnhlr
       61 lmallrqvac yaashppesf rtsrlpvvhs stlagrdqpv ymatpvdalf falaplykhn
      121 drfwplkdiv edclllkvir scsdrmqhia dveglgkdqv ycyneskcla wltkkvkava
      181 arlqsigipp paacssaasa gssggiqpts edcmvaahai varyippmla tvlksslgld
      241 trpattveee kqktpslpql kkkkfkqvqq qesnnkgapk nsvqksakkk kskqvqqqen
      301 nnegapensv qksakkkksk qvqqqesnnk gapensvqks akkkkskqvq qqesnnkgsp
      361 knseqksakk kkskqvqqqe snnkgspkns eqksakarsq eflsgetqdt kddsqteere
      421 gsqswheehf
//
LOCUS       XP_077504771             428 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114734 isoform X8 [Amblyomma
            americanum].
ACCESSION   XP_077504771
VERSION     XP_077504771.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648645.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..428
                     /product="uncharacterized protein LOC144114734 isoform X8"
                     /calculated_mol_wt=47394
     Region          21..>211
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     Site            order(23..25,40,63,103..105,112..113)
                     /site_type="other"
                     /note="heterotrimeric interface"
                     /db_xref="CDD:187751"
     CDS             1..428
                     /gene="LOC144114734"
                     /coded_by="XM_077648645.1:93..1379"
                     /db_xref="GeneID:144114734"
ORIGIN      
        1 marqlkneav sseddsepsr lfllpshcat gserrhyifp lprpnteehs mfmatdsgdl
       61 melakyqmeh esftadgpfe talhvfsrhg wqvacyaash ppesfrtsrl pvvhsstlag
      121 rdqpvymatp vdalffalap lykhndrfwp lkdivedcll lkvirscsdr mqhiadvegl
      181 gkdqvycyne skclawltkk vkavaarlqs igipppaacs saasagssgg iqptsedcmv
      241 aahaivaryi ppmlatvlks slgldtrpat tveeekqktp slpqlkkkkf kqvqqqesnn
      301 kgapknsvqk sakkkkskqv qqqennnega pensvqksak kkkskqvqqq esnnkgapen
      361 svqksakkkk skqvqqqesn nkgspknseq ksakarsqef lsgetqdtkd gsqteeregs
      421 qswheehf
//
LOCUS       XP_077504772             428 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114734 isoform X9 [Amblyomma
            americanum].
ACCESSION   XP_077504772
VERSION     XP_077504772.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648646.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..428
                     /product="uncharacterized protein LOC144114734 isoform X9"
                     /calculated_mol_wt=47453
     Region          21..>211
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     Site            order(23..25,40,63,103..105,112..113)
                     /site_type="other"
                     /note="heterotrimeric interface"
                     /db_xref="CDD:187751"
     CDS             1..428
                     /gene="LOC144114734"
                     /coded_by="XM_077648646.1:93..1379"
                     /db_xref="GeneID:144114734"
ORIGIN      
        1 marqlkneav sseddsepsr lfllpshcat gserrhyifp lprpnteehs mfmatdsgdl
       61 melakyqmeh esftadgpfe talhvfsrhg wqvacyaash ppesfrtsrl pvvhsstlag
      121 rdqpvymatp vdalffalap lykhndrfwp lkdivedcll lkvirscsdr mqhiadvegl
      181 gkdqvycyne skclawltkk vkavaarlqs igipppaacs saasagssgg iqptsedcmv
      241 aahaivaryi ppmlatvlks slgldtrpat tveeekqktp slpqlkkkkf kqvqqqesnn
      301 kgapknsvqk sakkkkskqv qqqennnega pensvqksak kkkskqvqqq esnnkgapen
      361 svqksakkkk skqvqqqesn nkgspknseq ksakarsqef lsgetqdtkd dsqteeregs
      421 qswheehf
//
LOCUS       XP_077504773             428 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114734 isoform X10 [Amblyomma
            americanum].
ACCESSION   XP_077504773
VERSION     XP_077504773.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648647.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..428
                     /product="uncharacterized protein LOC144114734 isoform
                     X10"
                     /calculated_mol_wt=47438
     Region          21..>211
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     Site            order(23..25,40,63,103..105,112..113)
                     /site_type="other"
                     /note="heterotrimeric interface"
                     /db_xref="CDD:187751"
     CDS             1..428
                     /gene="LOC144114734"
                     /coded_by="XM_077648647.1:93..1379"
                     /db_xref="GeneID:144114734"
ORIGIN      
        1 marqlkneav sseddsepsr lfllpshcat gserrhyifp lprpnteehs mfmatdsgdl
       61 melakyqmeh esftadgpfe talhvfsrhg wqvacyaash ppesfrtsrl pvvhsstlag
      121 rdqpvymatp vdalffalap lykhndrfwp lkdivedcll lkvirscsdr mqhiadvegl
      181 gkdqvycyne skclawltkk vkavaarlqs igipppaacs saasagssgg iqptsedcmv
      241 aahaivaryi ppmlatvlks slgldtrpat tveeekqktp slpqlkkkks kqvqqqennn
      301 egapensvqk sakkkkskqv qqqesnnkga pensvqksak kkkskqvqqq esnnkgspkn
      361 seqksakkkk skqvqqqesn nkgspknseq ksakarsqef lsgetqdtkd dsqteeregs
      421 qswheehf
//
LOCUS       XP_077504774             422 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114734 isoform X11 [Amblyomma
            americanum].
ACCESSION   XP_077504774
VERSION     XP_077504774.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648648.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..422
                     /product="uncharacterized protein LOC144114734 isoform
                     X11"
                     /calculated_mol_wt=46580
     Region          21..>211
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     Site            order(23..25,40,63,103..105,112..113)
                     /site_type="other"
                     /note="heterotrimeric interface"
                     /db_xref="CDD:187751"
     CDS             1..422
                     /gene="LOC144114734"
                     /coded_by="XM_077648648.1:93..1361"
                     /db_xref="GeneID:144114734"
ORIGIN      
        1 marqlkneav sseddsepsr lfllpshcat gserrhyifp lprpnteehs mfmatdsgdl
       61 melakyqmeh esftadgpfe talhvfsrhg wqvacyaash ppesfrtsrl pvvhsstlag
      121 rdqpvymatp vdalffalap lykhndrfwp lkdivedcll lkvirscsdr mqhiadvegl
      181 gkdqvycyne skclawltkk vkavaarlqs igipppaacs saasagssgg iqptsedcmv
      241 aahaivaryi ppmlatvlks slgldtrpat tveeekqktp slpqlkkkkf kqvqqqesnn
      301 kgapknsvqk sakkkkskqv qqqennnega pensvqksak kkkskqvqqq esnnkgapen
      361 svqksakkkk skqvqqqesn nkgspknseq ksaktprtav rqkrekaaka gmksissyfa
      421 kk
//
LOCUS       XP_077504775             415 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114734 isoform X12 [Amblyomma
            americanum].
ACCESSION   XP_077504775
VERSION     XP_077504775.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648649.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..415
                     /product="uncharacterized protein LOC144114734 isoform
                     X12"
                     /calculated_mol_wt=46080
     Region          21..>171
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     Site            order(23..25,40,63,71..72,73..74)
                     /site_type="other"
                     /note="heterotrimeric interface"
                     /db_xref="CDD:187751"
     CDS             1..415
                     /gene="LOC144114734"
                     /coded_by="XM_077648649.1:93..1340"
                     /db_xref="GeneID:144114734"
ORIGIN      
        1 marqlkneav sseddsepsr lfllpshcat gserrhyifp lprpnteehs mfmatdsgdl
       61 melakyqmeh esftadgpfe tdqpvymatp vdalffalap lykhndrfwp lkdivedcll
      121 lkvirscsdr mqhiadvegl gkdqvycyne skclawltkk vkavaarlqs igipppaacs
      181 saasagssgg iqptsedcmv aahaivaryi ppmlatvlks slgldtrpat tveeekqktp
      241 slpqlkkkkf kqvqqqesnn kgapknsvqk sakkkkskqv qqqennnega pensvqksak
      301 kkkskqvqqq esnnkgapen svqksakkkk skqvqqqesn nkgspknseq ksakkkkskq
      361 vqqqesnnkg spknseqksa karsqeflsg etqdtkddsq teeregsqsw heehf
//
LOCUS       XP_077504776             414 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114734 isoform X13 [Amblyomma
            americanum].
ACCESSION   XP_077504776
VERSION     XP_077504776.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648650.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..414
                     /product="uncharacterized protein LOC144114734 isoform
                     X13"
                     /calculated_mol_wt=45951
     Region          21..>170
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     Site            order(23..25,40,62,70..71,72..73)
                     /site_type="other"
                     /note="heterotrimeric interface"
                     /db_xref="CDD:187751"
     CDS             1..414
                     /gene="LOC144114734"
                     /coded_by="XM_077648650.1:67..1311"
                     /db_xref="GeneID:144114734"
ORIGIN      
        1 marqlkneav sseddsepsr lfllpshcat gserrhyifp lprpntehsm fmatdsgdlm
       61 elakyqmehe sftadgpfet dqpvymatpv dalffalapl ykhndrfwpl kdivedclll
      121 kvirscsdrm qhiadveglg kdqvycynes kclawltkkv kavaarlqsi gipppaacss
      181 aasagssggi qptsedcmva ahaivaryip pmlatvlkss lgldtrpatt veeekqktps
      241 lpqlkkkkfk qvqqqesnnk gapknsvqks akkkkskqvq qqennnegap ensvqksakk
      301 kkskqvqqqe snnkgapens vqksakkkks kqvqqqesnn kgspknseqk sakkkkskqv
      361 qqqesnnkgs pknseqksak arsqeflsge tqdtkddsqt eeregsqswh eehf
//
LOCUS       XP_077504777             411 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114734 isoform X14 [Amblyomma
            americanum].
ACCESSION   XP_077504777
VERSION     XP_077504777.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648651.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..411
                     /product="uncharacterized protein LOC144114734 isoform
                     X14"
                     /calculated_mol_wt=46883
     Region          21..>211
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     Site            order(23..25,40,63,103..105,112..113)
                     /site_type="other"
                     /note="heterotrimeric interface"
                     /db_xref="CDD:187751"
     Region          303..>403
                     /region_name="U2AF_lg"
                     /note="U2 snRNP auxilliary factor, large subunit, splicing
                     factor; TIGR01642"
                     /db_xref="CDD:273727"
     CDS             1..411
                     /gene="LOC144114734"
                     /coded_by="XM_077648651.1:93..1328"
                     /db_xref="GeneID:144114734"
ORIGIN      
        1 marqlkneav sseddsepsr lfllpshcat gserrhyifp lprpnteehs mfmatdsgdl
       61 melakyqmeh esftadgpfe talhvfsrhg wqvacyaash ppesfrtsrl pvvhsstlag
      121 rdqpvymatp vdalffalap lykhndrfwp lkdivedcll lkvirscsdr mqhiadvegl
      181 gkdqvycyne skclawltkk vkavaarlqs igipppaacs saasagssgg iqptsawiqd
      241 qpppsrrksk khqachslrk rssnkcssrk vttkahrril crsqprkrsp nkcsnrritt
      301 kahrrilcrs qprkrspnkc snrrvttkah rrilcrsqpr krspnkcsnr rvttkahrri
      361 lsrsqprkrs pnkcsnrrvt tkahrrilsr sqprhqgrqs drrerrqpkl a
//
LOCUS       XP_077504778             407 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114734 isoform X15 [Amblyomma
            americanum].
ACCESSION   XP_077504778
VERSION     XP_077504778.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648652.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..407
                     /product="uncharacterized protein LOC144114734 isoform
                     X15"
                     /calculated_mol_wt=46156
     Region          21..>211
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     Site            order(23..25,40,63,103..105,112..113)
                     /site_type="other"
                     /note="heterotrimeric interface"
                     /db_xref="CDD:187751"
     CDS             1..407
                     /gene="LOC144114734"
                     /coded_by="XM_077648652.1:93..1316"
                     /db_xref="GeneID:144114734"
ORIGIN      
        1 marqlkneav sseddsepsr lfllpshcat gserrhyifp lprpnteehs mfmatdsgdl
       61 melakyqmeh esftadgpfe talhvfsrhg wqvacyaash ppesfrtsrl pvvhsstlag
      121 rdqpvymatp vdalffalap lykhndrfwp lkdivedcll lkvirscsdr mqhiadvegl
      181 gkdqvycyne skclawltkk vkavaarlqs igipppaacs saasagssgg iqptsawiqd
      241 qpppsrrksk khqachslrk rssnkcssrk vttkahrril crsqprkrsp nkcsnrritt
      301 kahrrilcrs qprkrspnkc snrrvttkah rrilcrsqpr krspnkcsnr rvttkahrri
      361 lsrsqprhva rnfflekpkt prtavrqkre kaakagmksi ssyfakk
//
LOCUS       XP_077504779             407 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114734 isoform X16 [Amblyomma
            americanum].
ACCESSION   XP_077504779
VERSION     XP_077504779.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648653.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..407
                     /product="uncharacterized protein LOC144114734 isoform
                     X16"
                     /calculated_mol_wt=46186
     Region          21..>211
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     Site            order(23..25,40,63,103..105,112..113)
                     /site_type="other"
                     /note="heterotrimeric interface"
                     /db_xref="CDD:187751"
     CDS             1..407
                     /gene="LOC144114734"
                     /coded_by="XM_077648653.1:93..1316"
                     /db_xref="GeneID:144114734"
ORIGIN      
        1 marqlkneav sseddsepsr lfllpshcat gserrhyifp lprpnteehs mfmatdsgdl
       61 melakyqmeh esftadgpfe talhvfsrhg wqvacyaash ppesfrtsrl pvvhsstlag
      121 rdqpvymatp vdalffalap lykhndrfwp lkdivedcll lkvirscsdr mqhiadvegl
      181 gkdqvycyne skclawltkk vkavaarlqs igipppaacs saasagssgg iqptsawiqd
      241 qpppsrrksk khqachslrk rssnkcssrk vttkahrril crsqprkrsp nkcsnrritt
      301 kahrrilcrs qprkrspnkc snrrvttkah rrilcrsqpr krspnkcsnr rvttkahrri
      361 lsrsqprhva rnfflekpkt prttvrqkre kaakagmksi ssyfakk
//
LOCUS       XP_077504780             405 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114734 isoform X17 [Amblyomma
            americanum].
ACCESSION   XP_077504780
VERSION     XP_077504780.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648654.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..405
                     /product="uncharacterized protein LOC144114734 isoform
                     X17"
                     /calculated_mol_wt=44760
     Region          42..>161
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     CDS             1..405
                     /gene="LOC144114734"
                     /coded_by="XM_077648654.1:255..1472"
                     /db_xref="GeneID:144114734"
ORIGIN      
        1 mfmatdsgdl melakyqmeh esftadgpfe talhvfsrhg wqvacyaash ppesfrtsrl
       61 pvvhsstlag rdqpvymatp vdalffalap lykhndrfwp lkdivedcll lkvirscsdr
      121 mqhiadvegl gkdqvycyne skclawltkk vkavaarlqs igipppaacs saasagssgg
      181 iqptsedcmv aahaivaryi ppmlatvlks slgldtrpat tveeekqktp slpqlkkkkf
      241 kqvqqqesnn kgapknsvqk sakkkkskqv qqqennnega pensvqksak kkkskqvqqq
      301 esnnkgapen svqksakkkk skqvqqqesn nkgspknseq ksakkkkskq vqqqesnnkg
      361 spknseqksa karsqeflsg etqdtkddsq teeregsqsw heehf
//
LOCUS       XP_077504781             401 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114734 isoform X18 [Amblyomma
            americanum].
ACCESSION   XP_077504781
VERSION     XP_077504781.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648655.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..401
                     /product="uncharacterized protein LOC144114734 isoform
                     X18"
                     /calculated_mol_wt=44471
     Region          21..>211
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     Site            order(23..25,40,63,103..105,112..113)
                     /site_type="other"
                     /note="heterotrimeric interface"
                     /db_xref="CDD:187751"
     CDS             1..401
                     /gene="LOC144114734"
                     /coded_by="XM_077648655.1:93..1298"
                     /db_xref="GeneID:144114734"
ORIGIN      
        1 marqlkneav sseddsepsr lfllpshcat gserrhyifp lprpnteehs mfmatdsgdl
       61 melakyqmeh esftadgpfe talhvfsrhg wqvacyaash ppesfrtsrl pvvhsstlag
      121 rdqpvymatp vdalffalap lykhndrfwp lkdivedcll lkvirscsdr mqhiadvegl
      181 gkdqvycyne skclawltkk vkavaarlqs igipppaacs saasagssgg iqptsedcmv
      241 aahaivaryi ppmlatvlks slgldtrpat tveeekqktp slpqlkkkkf kqvqqqesnn
      301 kgapknsvqk sakkkkskqv qqqennnega pensvqksak kkkskqvqqq esnnkgspkn
      361 seqksakars qeflsgetqd tkddsqteer egsqswheeh f
//
LOCUS       XP_077504782             401 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114734 isoform X19 [Amblyomma
            americanum].
ACCESSION   XP_077504782
VERSION     XP_077504782.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648656.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..401
                     /product="uncharacterized protein LOC144114734 isoform
                     X19"
                     /calculated_mol_wt=44443
     Region          21..>211
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     Site            order(23..25,40,63,103..105,112..113)
                     /site_type="other"
                     /note="heterotrimeric interface"
                     /db_xref="CDD:187751"
     CDS             1..401
                     /gene="LOC144114734"
                     /coded_by="XM_077648656.1:93..1298"
                     /db_xref="GeneID:144114734"
ORIGIN      
        1 marqlkneav sseddsepsr lfllpshcat gserrhyifp lprpnteehs mfmatdsgdl
       61 melakyqmeh esftadgpfe talhvfsrhg wqvacyaash ppesfrtsrl pvvhsstlag
      121 rdqpvymatp vdalffalap lykhndrfwp lkdivedcll lkvirscsdr mqhiadvegl
      181 gkdqvycyne skclawltkk vkavaarlqs igipppaacs saasagssgg iqptsedcmv
      241 aahaivaryi ppmlatvlks slgldtrpat tveeekqktp slpqlkkkkf kqvqqqesnn
      301 kgapknsvqk sakkkkskqv qqqesnnkga pensvqksak kkkskqvqqq esnnkgspkn
      361 seqksakars qeflsgetqd tkddsqteer egsqswheeh f
//
LOCUS       XP_077504783             401 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114734 isoform X20 [Amblyomma
            americanum].
ACCESSION   XP_077504783
VERSION     XP_077504783.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648657.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..401
                     /product="uncharacterized protein LOC144114734 isoform
                     X20"
                     /calculated_mol_wt=44488
     Region          21..>211
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     Site            order(23..25,40,63,103..105,112..113)
                     /site_type="other"
                     /note="heterotrimeric interface"
                     /db_xref="CDD:187751"
     CDS             1..401
                     /gene="LOC144114734"
                     /coded_by="XM_077648657.1:93..1298"
                     /db_xref="GeneID:144114734"
ORIGIN      
        1 marqlkneav sseddsepsr lfllpshcat gserrhyifp lprpnteehs mfmatdsgdl
       61 melakyqmeh esftadgpfe talhvfsrhg wqvacyaash ppesfrtsrl pvvhsstlag
      121 rdqpvymatp vdalffalap lykhndrfwp lkdivedcll lkvirscsdr mqhiadvegl
      181 gkdqvycyne skclawltkk vkavaarlqs igipppaacs saasagssgg iqptsedcmv
      241 aahaivaryi ppmlatvlks slgldtrpat tveeekqktp slpqlkkkkf kqvqqqesnn
      301 kgapknsvqk sakkkkskqv qqqesnnkgs pknseqksak kkkskqvqqq esnnkgspkn
      361 seqksakars qeflsgetqd tkddsqteer egsqswheeh f
//
LOCUS       XP_077504784             395 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114734 isoform X21 [Amblyomma
            americanum].
ACCESSION   XP_077504784
VERSION     XP_077504784.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648658.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..395
                     /product="uncharacterized protein LOC144114734 isoform
                     X21"
                     /calculated_mol_wt=43582
     Region          21..>211
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     Site            order(23..25,40,63,103..105,112..113)
                     /site_type="other"
                     /note="heterotrimeric interface"
                     /db_xref="CDD:187751"
     CDS             1..395
                     /gene="LOC144114734"
                     /coded_by="XM_077648658.1:93..1280"
                     /db_xref="GeneID:144114734"
ORIGIN      
        1 marqlkneav sseddsepsr lfllpshcat gserrhyifp lprpnteehs mfmatdsgdl
       61 melakyqmeh esftadgpfe talhvfsrhg wqvacyaash ppesfrtsrl pvvhsstlag
      121 rdqpvymatp vdalffalap lykhndrfwp lkdivedcll lkvirscsdr mqhiadvegl
      181 gkdqvycyne skclawltkk vkavaarlqs igipppaacs saasagssgg iqptsedcmv
      241 aahaivaryi ppmlatvlks slgldtrpat tveeekqktp slpqlkkkkf kqvqqqesnn
      301 kgapknsvqk sakkkkskqv qqqennnega pensvqksak kkkskqvqqq esnnkgapen
      361 svqksaktpr tavrqkreka akagmksiss yfvkk
//
LOCUS       XP_077504785             384 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114734 isoform X22 [Amblyomma
            americanum].
ACCESSION   XP_077504785
VERSION     XP_077504785.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648659.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..384
                     /product="uncharacterized protein LOC144114734 isoform
                     X22"
                     /calculated_mol_wt=43667
     Region          21..>211
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     Site            order(23..25,40,63,103..105,112..113)
                     /site_type="other"
                     /note="heterotrimeric interface"
                     /db_xref="CDD:187751"
     Region          276..>376
                     /region_name="U2AF_lg"
                     /note="U2 snRNP auxilliary factor, large subunit, splicing
                     factor; TIGR01642"
                     /db_xref="CDD:273727"
     CDS             1..384
                     /gene="LOC144114734"
                     /coded_by="XM_077648659.1:93..1247"
                     /db_xref="GeneID:144114734"
ORIGIN      
        1 marqlkneav sseddsepsr lfllpshcat gserrhyifp lprpnteehs mfmatdsgdl
       61 melakyqmeh esftadgpfe talhvfsrhg wqvacyaash ppesfrtsrl pvvhsstlag
      121 rdqpvymatp vdalffalap lykhndrfwp lkdivedcll lkvirscsdr mqhiadvegl
      181 gkdqvycyne skclawltkk vkavaarlqs igipppaacs saasagssgg iqptsawiqd
      241 qpppsrrksk khqachslrk rssnkcssrk vttkahrril crsqprkrsp nkcsnrritt
      301 kahrrilcrs qprkrspnkc snrrvttkah rrilcrsqpr krspnkcsnr rvttkahrri
      361 lsrsqprhqg rqsdrrerrq pkla
//
LOCUS       XP_077504786             380 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114734 isoform X23 [Amblyomma
            americanum].
ACCESSION   XP_077504786
VERSION     XP_077504786.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648660.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..380
                     /product="uncharacterized protein LOC144114734 isoform
                     X23"
                     /calculated_mol_wt=42955
     Region          21..>211
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     Site            order(23..25,40,63,103..105,112..113)
                     /site_type="other"
                     /note="heterotrimeric interface"
                     /db_xref="CDD:187751"
     CDS             1..380
                     /gene="LOC144114734"
                     /coded_by="XM_077648660.1:93..1235"
                     /db_xref="GeneID:144114734"
ORIGIN      
        1 marqlkneav sseddsepsr lfllpshcat gserrhyifp lprpnteehs mfmatdsgdl
       61 melakyqmeh esftadgpfe talhvfsrhg wqvacyaash ppesfrtsrl pvvhsstlag
      121 rdqpvymatp vdalffalap lykhndrfwp lkdivedcll lkvirscsdr mqhiadvegl
      181 gkdqvycyne skclawltkk vkavaarlqs igipppaacs saasagssgg iqptsawiqd
      241 qpppsrrksk khqachslrk rssnkcssrk vttkahrril crsqprkrsp nkcsnrritt
      301 kahrrilcrs qprkrspnkc snrrvttkah rrilsrsqpr hvarnfflek pktprttvrq
      361 krekaakagm ksissyfakk
//
LOCUS       XP_077504787             380 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114734 isoform X24 [Amblyomma
            americanum].
ACCESSION   XP_077504787
VERSION     XP_077504787.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648661.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..380
                     /product="uncharacterized protein LOC144114734 isoform
                     X24"
                     /calculated_mol_wt=42941
     Region          21..>211
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     Site            order(23..25,40,63,103..105,112..113)
                     /site_type="other"
                     /note="heterotrimeric interface"
                     /db_xref="CDD:187751"
     CDS             1..380
                     /gene="LOC144114734"
                     /coded_by="XM_077648661.1:93..1235"
                     /db_xref="GeneID:144114734"
ORIGIN      
        1 marqlkneav sseddsepsr lfllpshcat gserrhyifp lprpnteehs mfmatdsgdl
       61 melakyqmeh esftadgpfe talhvfsrhg wqvacyaash ppesfrtsrl pvvhsstlag
      121 rdqpvymatp vdalffalap lykhndrfwp lkdivedcll lkvirscsdr mqhiadvegl
      181 gkdqvycyne skclawltkk vkavaarlqs igipppaacs saasagssgg iqptsawiqd
      241 qpppsrrksk khqachslrk rssnkcssrk vttkahrril crsqprkrsp nkcsnrrvtt
      301 kahrrilcrs qprkrspnkc snrrvttkah rrilsrsqpr hvarnfflek pktprttvrq
      361 krekaakagm ksissyfakk
//
LOCUS       XP_077504788             380 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114734 isoform X25 [Amblyomma
            americanum].
ACCESSION   XP_077504788
VERSION     XP_077504788.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648662.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..380
                     /product="uncharacterized protein LOC144114734 isoform
                     X25"
                     /calculated_mol_wt=42924
     Region          21..>211
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     Site            order(23..25,40,63,103..105,112..113)
                     /site_type="other"
                     /note="heterotrimeric interface"
                     /db_xref="CDD:187751"
     CDS             1..380
                     /gene="LOC144114734"
                     /coded_by="XM_077648662.1:93..1235"
                     /db_xref="GeneID:144114734"
ORIGIN      
        1 marqlkneav sseddsepsr lfllpshcat gserrhyifp lprpnteehs mfmatdsgdl
       61 melakyqmeh esftadgpfe talhvfsrhg wqvacyaash ppesfrtsrl pvvhsstlag
      121 rdqpvymatp vdalffalap lykhndrfwp lkdivedcll lkvirscsdr mqhiadvegl
      181 gkdqvycyne skclawltkk vkavaarlqs igipppaacs saasagssgg iqptsawiqd
      241 qpppsrrksk khqachslrk rssnkcssrk vttkahrril crsqprkrsp nkcsnrrvtt
      301 kahrrilsrs qprkrspnkc snrrvttkah rrilsrsqpr hvarnfflek pktprttvrq
      361 krekaakagm ksissyfakk
//
LOCUS       XP_077504789             375 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114734 isoform X26 [Amblyomma
            americanum].
ACCESSION   XP_077504789
VERSION     XP_077504789.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648663.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..375
                     /product="uncharacterized protein LOC144114734 isoform
                     X26"
                     /calculated_mol_wt=41405
     Region          12..>131
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     CDS             1..375
                     /gene="LOC144114734"
                     /coded_by="XM_077648663.1:312..1439"
                     /db_xref="GeneID:144114734"
ORIGIN      
        1 mnhlrlmall rqvacyaash ppesfrtsrl pvvhsstlag rdqpvymatp vdalffalap
       61 lykhndrfwp lkdivedcll lkvirscsdr mqhiadvegl gkdqvycyne skclawltkk
      121 vkavaarlqs igipppaacs saasagssgg iqptsedcmv aahaivaryi ppmlatvlks
      181 slgldtrpat tveeekqktp slpqlkkkkf kqvqqqesnn kgapknsvqk sakkkkskqv
      241 qqqennnega pensvqksak kkkskqvqqq esnnkgapen svqksakkkk skqvqqqesn
      301 nkgspknseq ksakkkkskq vqqqesnnkg spknseqksa karsqeflsg etqdtkddsq
      361 teeregsqsw heehf
//
LOCUS       XP_077504790             368 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114734 isoform X27 [Amblyomma
            americanum].
ACCESSION   XP_077504790
VERSION     XP_077504790.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648664.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..368
                     /product="uncharacterized protein LOC144114734 isoform
                     X27"
                     /calculated_mol_wt=40573
     Region          21..>211
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     Site            order(23..25,40,63,103..105,112..113)
                     /site_type="other"
                     /note="heterotrimeric interface"
                     /db_xref="CDD:187751"
     CDS             1..368
                     /gene="LOC144114734"
                     /coded_by="XM_077648664.1:93..1199"
                     /db_xref="GeneID:144114734"
ORIGIN      
        1 marqlkneav sseddsepsr lfllpshcat gserrhyifp lprpnteehs mfmatdsgdl
       61 melakyqmeh esftadgpfe talhvfsrhg wqvacyaash ppesfrtsrl pvvhsstlag
      121 rdqpvymatp vdalffalap lykhndrfwp lkdivedcll lkvirscsdr mqhiadvegl
      181 gkdqvycyne skclawltkk vkavaarlqs igipppaacs saasagssgg iqptsedcmv
      241 aahaivaryi ppmlatvlks slgldtrpat tveeekqktp slpqlkkkkf kqvqqqesnn
      301 kgapknsvqk sakkkkskqv qqqesnnkga pensvqksak tprtavrqkr ekaakagmks
      361 issyfvkk
//
LOCUS       XP_077504791             253 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114738 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504791
VERSION     XP_077504791.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648665.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..253
                     /product="uncharacterized protein LOC144114738 isoform X1"
                     /calculated_mol_wt=27727
     CDS             1..253
                     /gene="LOC144114738"
                     /coded_by="XM_077648665.1:74..835"
                     /db_xref="GeneID:144114738"
ORIGIN      
        1 mqvteticae lfnrfnhghr atwvqpgvgp ttfiiwqvas vliatvtcml palltshwsv
       61 psrhapewkk seldtdfvtv iaavqapsvd aweldrrwaa qatgtsiyqq dikgqppsat
      121 lcglggcatk llanpmvfam qslstgilph kwkvgkvvpv yksataflts ssrvhctkhp
      181 tsyaactyls alvirfklfv hapvllysqp sfaraasdwn glphrvaait cpsrfhqelt
      241 qlmichirsp hli
//
LOCUS       XP_077504792             242 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114738 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504792
VERSION     XP_077504792.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648666.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..242
                     /product="uncharacterized protein LOC144114738 isoform X2"
                     /calculated_mol_wt=25340
     CDS             1..242
                     /gene="LOC144114738"
                     /coded_by="XM_077648666.1:74..802"
                     /db_xref="GeneID:144114738"
ORIGIN      
        1 mqvteticae lfnrfnhghr atwvqpgvgp ttfiiwqvas vliatvtcml palltshwsv
       61 psrhapewkk seldtdfvtv iaavqapsvd aweldrrwaa qatgtsiyqq dikgqppsat
      121 lcglggcatk llanpmvfam qgevlqgadp wtgapcsaaf adpawgvdgp aigsvkaala
      181 gagvlcgatp lsttsakycf avkenvfhgf lhssrsagls hihqngdpsk hicasfataa
      241 tl
//
LOCUS       XP_077504793             205 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114738 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504793
VERSION     XP_077504793.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648667.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..205
                     /product="uncharacterized protein LOC144114738 isoform X3"
                     /calculated_mol_wt=22400
     CDS             1..205
                     /gene="LOC144114738"
                     /coded_by="XM_077648667.1:103..720"
                     /db_xref="GeneID:144114738"
ORIGIN      
        1 mlpalltshw svpsrhapew kkseldtdfv tviaavqaps vdaweldrrw aaqatgtsiy
       61 qqdikgqpps atlcglggca tkllanpmvf amqslstgil phkwkvgkvv pvyksatafl
      121 tsssrvhctk hptsyaacty lsalvirfkl fvhapvllys qpsfaraasd wnglphrvaa
      181 itcpsrfhqe ltqlmichir sphli
//
LOCUS       XP_077504795             195 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114738 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077504795
VERSION     XP_077504795.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648669.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..195
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..195
                     /product="uncharacterized protein LOC144114738 isoform X4"
                     /calculated_mol_wt=21114
     CDS             1..195
                     /gene="LOC144114738"
                     /coded_by="XM_077648669.1:74..661"
                     /db_xref="GeneID:144114738"
ORIGIN      
        1 mqvteticae lfnrfnhghr atwvqpgvgp ttfiiwqvas vliatvtcml palltshwsv
       61 psrhapewkk seldtdfvtv iaavqapsvd aweldrrwaa qatgtsiyqq dikgqppsat
      121 lcglggcatk llanpmvfam qcltgtagvc fqrseaaave vpvtslntkt qakqndyaay
      181 vcsriqlfaq temhc
//
LOCUS       XP_077504796             242 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114739 [Amblyomma americanum].
ACCESSION   XP_077504796
VERSION     XP_077504796.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648670.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..242
                     /product="uncharacterized protein LOC144114739"
                     /calculated_mol_wt=27057
     Region          <1..>41
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     CDS             1..242
                     /gene="LOC144114739"
                     /coded_by="XM_077648670.1:543..1271"
                     /db_xref="GeneID:144114739"
ORIGIN      
        1 mqhiadvegh gedqvyryne skclawltkk vkavaarlqs igipppavcs saasagcsgg
       61 sqptsedfmv aahatvaryi ppmlatvlns slgkrssnkc ssrkvttkah rrilcrsqpr
      121 rscgqcqvls nfaelecvcy reidrvveah pedcttehpe fsflclnitv lraayfelpa
      181 qqetmsddih kiyrytacrq fvrwlwrrla kkhrlilpac vvakirasfp adtptgfrya
      241 ey
//
LOCUS       XP_077504797             242 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114739 [Amblyomma americanum].
ACCESSION   XP_077504797
VERSION     XP_077504797.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648671.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..242
                     /product="uncharacterized protein LOC144114739"
                     /calculated_mol_wt=27057
     Region          <1..>41
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     CDS             1..242
                     /gene="LOC144114739"
                     /coded_by="XM_077648671.1:523..1251"
                     /db_xref="GeneID:144114739"
ORIGIN      
        1 mqhiadvegh gedqvyryne skclawltkk vkavaarlqs igipppavcs saasagcsgg
       61 sqptsedfmv aahatvaryi ppmlatvlns slgkrssnkc ssrkvttkah rrilcrsqpr
      121 rscgqcqvls nfaelecvcy reidrvveah pedcttehpe fsflclnitv lraayfelpa
      181 qqetmsddih kiyrytacrq fvrwlwrrla kkhrlilpac vvakirasfp adtptgfrya
      241 ey
//
LOCUS       XP_077504798             242 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114739 [Amblyomma americanum].
ACCESSION   XP_077504798
VERSION     XP_077504798.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648672.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..242
                     /product="uncharacterized protein LOC144114739"
                     /calculated_mol_wt=27057
     Region          <1..>41
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     CDS             1..242
                     /gene="LOC144114739"
                     /coded_by="XM_077648672.1:621..1349"
                     /db_xref="GeneID:144114739"
ORIGIN      
        1 mqhiadvegh gedqvyryne skclawltkk vkavaarlqs igipppavcs saasagcsgg
       61 sqptsedfmv aahatvaryi ppmlatvlns slgkrssnkc ssrkvttkah rrilcrsqpr
      121 rscgqcqvls nfaelecvcy reidrvveah pedcttehpe fsflclnitv lraayfelpa
      181 qqetmsddih kiyrytacrq fvrwlwrrla kkhrlilpac vvakirasfp adtptgfrya
      241 ey
//
LOCUS       XP_077504799             187 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114740 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504799
VERSION     XP_077504799.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648673.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="uncharacterized protein LOC144114740 isoform X1"
                     /calculated_mol_wt=20859
     Region          54..>169
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     Site            order(55..57,72,95,103..104,105..106)
                     /site_type="other"
                     /note="heterotrimeric interface"
                     /db_xref="CDD:187751"
     CDS             1..187
                     /gene="LOC144114740"
                     /coded_by="XM_077648673.1:1797..2360"
                     /db_xref="GeneID:144114740"
ORIGIN      
        1 mpypylsglw gadiqnranc asacvscnrc rsffvmarql knetvssedd sepfllpnhc
       61 atgserrhyi splprpntee hsmfmatdsg dlmelakyqm ehesftadgp letdqpvyma
      121 tpvdalffal aplykhndrf wplkdivedc lllkvirscs drmqhiadve gccaikngdv
      181 tvpagtm
//
LOCUS       XP_077504800             143 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114740 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504800
VERSION     XP_077504800.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648674.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..143
                     /product="uncharacterized protein LOC144114740 isoform X2"
                     /calculated_mol_wt=16485
     CDS             1..143
                     /gene="LOC144114740"
                     /coded_by="XM_077648674.1:1942..2373"
                     /db_xref="GeneID:144114740"
ORIGIN      
        1 mtrsrsccqv reqtklcpcf cvrrndassh qpfhattapl evseditfph cpvqiqkntr
       61 cswrltrvts wswrntrwsm nhlrlmallr qhndrfwplk divedclllk virscsdrmq
      121 hiadvegcca ikngdvtvpa gtm
//
LOCUS       XP_077504801             140 aa            linear   INV 25-NOV-2025
DEFINITION  ribonuclease H2 subunit B-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077504801
VERSION     XP_077504801.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648675.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..140
                     /product="ribonuclease H2 subunit B-like isoform X3"
                     /calculated_mol_wt=15662
     Region          26..>122
                     /region_name="RNase_H2-B"
                     /note="Ribonuclease H2-B is a subunit of the eukaryotic
                     RNase H complex which cleaves RNA-DNA hybrids; cl18968"
                     /db_xref="CDD:418515"
     CDS             1..140
                     /gene="LOC144114740"
                     /coded_by="XM_077648675.1:1942..2364"
                     /db_xref="GeneID:144114740"
ORIGIN      
        1 mtrsrsccqv hhcatgserr hyisplprpn teehsmfmat dsgdlmelak yqmehesfta
       61 dgpletdqpv ymatpvdalf falaplykhn drfwplkdiv edclllkvir scsdrmqhia
      121 dvegccaikn gdvtvpagtm
//
LOCUS       XP_077504802             130 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114740 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077504802
VERSION     XP_077504802.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648676.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..130
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..130
                     /product="uncharacterized protein LOC144114740 isoform X4"
                     /calculated_mol_wt=14228
     CDS             1..130
                     /gene="LOC144114740"
                     /coded_by="XM_077648676.1:1797..2189"
                     /db_xref="GeneID:144114740"
ORIGIN      
        1 mpypylsglw gadiqnranc asacvscnrc rsffvmarql knetvssedd sepfllpnhc
       61 atgserrhyi splprpntee hsmfmatdsg dlmelakyqm ehesftadgp letgccaikn
      121 gdvtvpagtm
//
LOCUS       XP_077504803             117 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114740 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077504803
VERSION     XP_077504803.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648677.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..117
                     /product="uncharacterized protein LOC144114740 isoform X5"
                     /calculated_mol_wt=13473
     CDS             1..117
                     /gene="LOC144114740"
                     /coded_by="XM_077648677.1:1942..2295"
                     /db_xref="GeneID:144114740"
ORIGIN      
        1 mtrsrsccqt taplevsedi tfphcpvqiq kntrcswrlt rvtswswrnt rwsmnhlrlm
       61 allrqhndrf wplkdivedc lllkvirscs drmqhiadve gccaikngdv tvpagtm
//
LOCUS       XP_077504804             136 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114742 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504804
VERSION     XP_077504804.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648678.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..136
                     /product="uncharacterized protein LOC144114742 isoform X1"
                     /calculated_mol_wt=15160
     CDS             1..136
                     /gene="LOC144114742"
                     /coded_by="XM_077648678.1:21..431"
                     /db_xref="GeneID:144114742"
ORIGIN      
        1 mpypylsglw gadiqnranc asacvscnrw rsffvmarql knetvssedd sepfllpkeh
       61 smfmatdsgd lmelakyqme hesftadgpf etdqpvymat pvdalffala plykdmartk
      121 ctatmslsal hglprk
//
LOCUS       XP_077504805             136 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114742 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504805
VERSION     XP_077504805.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648679.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..136
                     /product="uncharacterized protein LOC144114742 isoform X1"
                     /calculated_mol_wt=15160
     CDS             1..136
                     /gene="LOC144114742"
                     /coded_by="XM_077648679.1:22..432"
                     /db_xref="GeneID:144114742"
ORIGIN      
        1 mpypylsglw gadiqnranc asacvscnrw rsffvmarql knetvssedd sepfllpkeh
       61 smfmatdsgd lmelakyqme hesftadgpf etdqpvymat pvdalffala plykdmartk
      121 ctatmslsal hglprk
//
LOCUS       XP_077504806             124 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114742 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504806
VERSION     XP_077504806.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648680.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..124
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..124
                     /product="uncharacterized protein LOC144114742 isoform X2"
                     /calculated_mol_wt=14539
     CDS             1..124
                     /gene="LOC144114742"
                     /coded_by="XM_077648680.1:20..394"
                     /db_xref="GeneID:144114742"
ORIGIN      
        1 mpypylsglw gadiqnranc asacvscnrw rsffvmarql knetvssedd sepfllpnhc
       61 atgserrhyf tfphcpvqiq kntrcswrlt rvtswswrnt rwsmnhlrlm allrqisqct
      121 wplq
//
LOCUS       XP_077504807              75 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114742 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504807
VERSION     XP_077504807.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648681.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..75
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..75
                     /product="uncharacterized protein LOC144114742 isoform X3"
                     /calculated_mol_wt=8224
     CDS             1..75
                     /gene="LOC144114742"
                     /coded_by="XM_077648681.1:348..575"
                     /db_xref="GeneID:144114742"
ORIGIN      
        1 mfmatdsgdl melakyqmeh esftadgpfe tdqpvymatp vdalffalap lykdmartkc
       61 tatmslsalh glprk
//
LOCUS       XP_077504808              75 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114742 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504808
VERSION     XP_077504808.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648682.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..75
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..75
                     /product="uncharacterized protein LOC144114742 isoform X3"
                     /calculated_mol_wt=8224
     CDS             1..75
                     /gene="LOC144114742"
                     /coded_by="XM_077648682.1:97..324"
                     /db_xref="GeneID:144114742"
ORIGIN      
        1 mfmatdsgdl melakyqmeh esftadgpfe tdqpvymatp vdalffalap lykdmartkc
       61 tatmslsalh glprk
//
LOCUS       XP_077504809              75 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114742 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504809
VERSION     XP_077504809.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648683.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..75
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..75
                     /product="uncharacterized protein LOC144114742 isoform X3"
                     /calculated_mol_wt=8224
     CDS             1..75
                     /gene="LOC144114742"
                     /coded_by="XM_077648683.1:137..364"
                     /db_xref="GeneID:144114742"
ORIGIN      
        1 mfmatdsgdl melakyqmeh esftadgpfe tdqpvymatp vdalffalap lykdmartkc
       61 tatmslsalh glprk
//
LOCUS       XP_077504810              75 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114742 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504810
VERSION     XP_077504810.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648684.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..75
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..75
                     /product="uncharacterized protein LOC144114742 isoform X3"
                     /calculated_mol_wt=8224
     CDS             1..75
                     /gene="LOC144114742"
                     /coded_by="XM_077648684.1:273..500"
                     /db_xref="GeneID:144114742"
ORIGIN      
        1 mfmatdsgdl melakyqmeh esftadgpfe tdqpvymatp vdalffalap lykdmartkc
       61 tatmslsalh glprk
//
LOCUS       XP_077504811             134 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114743 [Amblyomma americanum].
ACCESSION   XP_077504811
VERSION     XP_077504811.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648685.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..134
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..134
                     /product="uncharacterized protein LOC144114743"
                     /calculated_mol_wt=14104
     Region          <25..>130
                     /region_name="SP1-4_N"
                     /note="N-terminal domain of transcription factor
                     Specificity Proteins (SP) 1-4; cl41773"
                     /db_xref="CDD:425404"
     CDS             1..134
                     /gene="LOC144114743"
                     /coded_by="XM_077648685.1:209..613"
                     /db_xref="GeneID:144114743"
ORIGIN      
        1 mdrhfnwvaw clmdfllaar flgclpindd grveeglelp vvnealdgse vllswedslp
       61 egsrrddlsq laasspiysa saavpvlqst gnssgdtsra sagtsksata garssagagt
      121 sakrrepqks rhkr
//
LOCUS       XP_077504812             134 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114743 [Amblyomma americanum].
ACCESSION   XP_077504812
VERSION     XP_077504812.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648686.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..134
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..134
                     /product="uncharacterized protein LOC144114743"
                     /calculated_mol_wt=14104
     Region          <25..>130
                     /region_name="SP1-4_N"
                     /note="N-terminal domain of transcription factor
                     Specificity Proteins (SP) 1-4; cl41773"
                     /db_xref="CDD:425404"
     CDS             1..134
                     /gene="LOC144114743"
                     /coded_by="XM_077648686.1:148..552"
                     /db_xref="GeneID:144114743"
ORIGIN      
        1 mdrhfnwvaw clmdfllaar flgclpindd grveeglelp vvnealdgse vllswedslp
       61 egsrrddlsq laasspiysa saavpvlqst gnssgdtsra sagtsksata garssagagt
      121 sakrrepqks rhkr
//
LOCUS       XP_077504813             580 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-Mannosidase class I b [Amblyomma americanum].
ACCESSION   XP_077504813
VERSION     XP_077504813.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648687.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (GKSP01012831.1) annotated
            using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 894 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..580
                     /product="alpha-Mannosidase class I b"
                     /calculated_mol_wt=65579
     Region          136..577
                     /region_name="Glyco_hydro_47"
                     /note="Glycosyl hydrolase family 47; pfam01532"
                     /db_xref="CDD:460241"
     CDS             1..580
                     /gene="alpha-Man-Ib"
                     /coded_by="XM_077648687.1:1..1743"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 894 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144114749"
ORIGIN      
        1 mrssdvesgl inlsgappsw agsftwrmgq rkslwrlwkq lsrlqrslll vlavaalvlv
       61 asrmgqgplp apvvqtgqpp eeeasrqrlp stsrspddsk vslghsslrv epatdsprll
      121 qqslslrerr eavvdafrha wkgykayawg hdhlrpmsrt ghtwfglglt ivdgmdtmyl
      181 mglheelaaa rdwvahslql dqhrdvnlfe ttirvlggll smhtlteehm yldkalelgr
      241 rllpgfrsas gvpysdvnla sgvahapawa pdssvsevas lqlefralsr asgdpqfekk
      301 sfavsellhr lpkkeglvpm finaetgrfn adstltlgar adsyyeyllk qwlqtgkais
      361 wlkedyldav egiqkhlvrr sepnkllfig elvrgrtfyp kmdhlvcyfp gtlmlghvvg
      421 gmpqshmela relmdtcirm ysmnptflsp eiahfnlkpq gvrdilikgn dahnllrpet
      481 leslwymyyf trnetyrdla wrifqgfekh ckvpgggytt igsvlnakqt gprdmmesff
      541 laetlkylyl lfseedvler ysptkylfnt eahllplyts
//
LOCUS       XP_077504814             109 aa            linear   INV 25-NOV-2025
DEFINITION  histone H2A.V-like [Amblyomma americanum].
ACCESSION   XP_077504814
VERSION     XP_077504814.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648688.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..109
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..109
                     /product="histone H2A.V-like"
                     /calculated_mol_wt=11729
     Region          <9..102
                     /region_name="PLN00154"
                     /note="histone H2A; Provisional"
                     /db_xref="CDD:177756"
     CDS             1..109
                     /gene="LOC144114750"
                     /coded_by="XM_077648688.1:4..333"
                     /db_xref="GeneID:144114750"
ORIGIN      
        1 msatmrppfp vgrihrhlkn cttghgrvga taavysasil kyltaevlel agnaskdmrv
       61 kritlrhlql aihgdeelds likatiaggg vvphihkylv gkkrsqktp
//
LOCUS       XP_077504815             807 aa            linear   INV 25-NOV-2025
DEFINITION  nuclear hormone receptor FTZ-F1 beta-like [Amblyomma americanum].
ACCESSION   XP_077504815
VERSION     XP_077504815.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648689.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..807
                     /product="nuclear hormone receptor FTZ-F1 beta-like"
                     /calculated_mol_wt=85052
     Region          396..488
                     /region_name="NR_DBD_Lrh-1_like"
                     /note="The DNA-binding domain of Lrh-1 like nuclear
                     receptor family like is composed of two C4-type zinc
                     fingers; cd07167"
                     /db_xref="CDD:143541"
     Site            order(396,399,413,416,432,438,448,451)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143541"
     Site            order(406..408,414..415,417,419,421..422,425,445..446,452,
                     465..467,470..476,482)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143541"
     Region          599..758
                     /region_name="NR_LBD_F2"
                     /note="Ligand-binding domain of nuclear receptor family 2;
                     cd06930"
                     /db_xref="CDD:132728"
     Site            order(599,602..603,606,609..610,639..640,643..644,647,650,
                     660,683)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132728"
     Site            order(614,618,628..629,631..632,635..636)
                     /site_type="other"
                     /note="coactivator recognition site [polypeptide binding]"
                     /db_xref="CDD:132728"
     CDS             1..807
                     /gene="LOC144114751"
                     /coded_by="XM_077648689.1:545..2968"
                     /db_xref="GeneID:144114751"
ORIGIN      
        1 mvvqcgmeev rvcpvapppp lgaapqddgd dpevmqvtge glavhlrpkk qrvngasqpg
       61 vtwgdteere rpmswegels deedddsgaq aaqpaaaasg vvvvrpgnsp mlverddgat
      121 spisltvssg adskgngtsd kcisrvnlpd ksssnhvity vapvspapst pygsatptvv
      181 pafperqqhs hnssfgegrl ggsgafqppp fgesprptss sssrssysps qspllggsrg
      241 gplglpprhg aassdgslys ptssplqgrh vslveavysp sqspsqgrhr ladglsptaa
      301 hlrnlsvsrt stsavpytpa steppypylv vqptpdtrst assasvassa sdesnasdls
      361 sptrrpsaeg gggdplgalg eapggvsrqq llsgpcpicg drisgfhygi fscesckgff
      421 krtvqnkkhy vclrgaacpv avatrkkcpa crfdkclrtg mkleairedr trggrstyqc
      481 syslpsqllg gdarlasdsg dlpvepipll qkesrslssg splegspqpf vgqqqppvvp
      541 slvqeivsve hlwhytdrei srlseqfghk vsatgatald aggpdspgal gadggpslls
      601 slcniadhrl ykivkwcksl plfrdiqvdd qiallinswc ellllsccyr stgtpneirv
      661 smgksvtlgq arhlgmgpvi ermlgltdll rrlqvdqref vclkviillt sdasglkepe
      721 kvrackkqvl ealqaytish ypsqpskfge lllcvpeler acqvskesla asrriassag
      781 assssapmad pqapsfnllf ellrgdh
//
LOCUS       XP_077504817             281 aa            linear   INV 25-NOV-2025
DEFINITION  S-formylglutathione hydrolase [Amblyomma americanum].
ACCESSION   XP_077504817
VERSION     XP_077504817.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648691.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..281
                     /product="S-formylglutathione hydrolase"
                     /calculated_mol_wt=31370
     Region          1..281
                     /region_name="PLN02442"
                     /note="S-formylglutathione hydrolase"
                     /db_xref="CDD:178061"
     CDS             1..281
                     /gene="LOC144114752"
                     /coded_by="XM_077648691.1:190..1035"
                     /db_xref="GeneID:144114752"
ORIGIN      
        1 mealtlvssn kcfngfqkvy shfsetlkcs mkfaiylppk sevtavplly wlsgltcteq
       61 nfiqkagaqr casemsvaiv cpdtsprgcn iegeddsydl gtgagfyvda tqgkwkenyk
      121 mysyvtkelp aivkknfpvd ttrqgifghs mgghgalica lknpgmyksv safapicnpi
      181 dcpwgkkafs nylgedhkaw eeydatclvq kykgpplwfl idqgkednfl kdqqllperf
      241 leaaqkngag itlrmqegyd hsyffiasfi eehirhhata l
//
LOCUS       XP_077504818             492 aa            linear   INV 25-NOV-2025
DEFINITION  aldehyde dehydrogenase, mitochondrial-like [Amblyomma americanum].
ACCESSION   XP_077504818
VERSION     XP_077504818.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648692.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..492
                     /product="aldehyde dehydrogenase, mitochondrial-like"
                     /calculated_mol_wt=53672
     Region          6..485
                     /region_name="ALDH_F1AB_F2_RALDH1"
                     /note="NAD+-dependent retinal dehydrogenase 1, ALDH
                     families 1A, 1B, and 2-like; cd07141"
                     /db_xref="CDD:143459"
     Site            order(64..65,69,72,74,78,118,121..122,126,128..129,
                     132..136,138..139,142..149,242,245,248..249,252..253,255,
                     260,426,428,435,438,440..447,454..456,459..462,464,467,
                     472,479..484)
                     /site_type="other"
                     /note="homotetrameric interface [polypeptide binding]"
                     /db_xref="CDD:143459"
     Site            order(156..159,183,186,216,220..221,234,237,240,260..261,
                     293,340,343,391)
                     /site_type="other"
                     /note="NAD binding site [chemical binding]"
                     /db_xref="CDD:143459"
     Site            order(160,259,290,293)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:143459"
     CDS             1..492
                     /gene="LOC144114753"
                     /coded_by="XM_077648692.1:142..1620"
                     /db_xref="GeneID:144114753"
ORIGIN      
        1 mppnrnppik ytqifinnef vnsvskktfq vlnpatgdvi aevqegdkpd vdkavkaare
       61 afkfgstwrt mdaskrglll nkfadllqrd keylaslevl nngkpyaeal fdmdcsidci
      121 ryyagwsdki hgktipidgn hisftrhepi gvcgqiipwn ypvlmvcwkl gpalctgnvv
      181 vlkpaeqtpl talycaslik eagfppgvvn vipgygptag gaiashphvd kvaftgstev
      241 gkliqeaagk sntkrvtlem ggksplvifd dadldqavei ahaavfanmg qcccagtrtf
      301 vqegiykdfv lkakqlaker vvgdpfdekt vqgpqidkeq yckildllks gkeqgakvec
      361 ggdavpgsrg lfiqptvfsd vrddmriake eifgpvqqil kfktldevie rcnnttyglg
      421 sgvltkdidk ammfaqgvqa gsvwincyda ttpqtpfval rcldkarelg yagineyvei
      481 ktitmqvpqk ns
//
LOCUS       XP_077504819             140 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114754 [Amblyomma americanum].
ACCESSION   XP_077504819
VERSION     XP_077504819.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648693.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..140
                     /product="uncharacterized protein LOC144114754"
                     /calculated_mol_wt=15089
     CDS             1..140
                     /gene="LOC144114754"
                     /coded_by="XM_077648693.1:279..701"
                     /db_xref="GeneID:144114754"
ORIGIN      
        1 mnrmtavlpa ilrarnssqa rdpthrppft altsvlccql pasigpcvlp afgcvippep
       61 fmlkakscme vlqatklrvi cardleqagk lvngqwysls kasffsetkt tflgswaiyl
      121 gtcgqdnarq hasanskkst
//
LOCUS       XP_077504820             140 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114755 [Amblyomma americanum].
ACCESSION   XP_077504820
VERSION     XP_077504820.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648694.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..140
                     /product="uncharacterized protein LOC144114755"
                     /calculated_mol_wt=15089
     CDS             1..140
                     /gene="LOC144114755"
                     /coded_by="XM_077648694.1:288..710"
                     /db_xref="GeneID:144114755"
ORIGIN      
        1 mnrmtavlpa ilrarnssqa rdpthrppft altsvlccql pasigpcvlp afgcvippep
       61 fmlkakscme vlqatklrvi cardleqagk lvngqwysls kasffsetkt tflgswaiyl
      121 gtcgqdnarq hasanskkst
//
LOCUS       XP_077504821             403 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114756 [Amblyomma americanum].
ACCESSION   XP_077504821
VERSION     XP_077504821.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648695.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..403
                     /product="uncharacterized protein LOC144114756"
                     /calculated_mol_wt=43355
     CDS             1..403
                     /gene="LOC144114756"
                     /coded_by="XM_077648695.1:221..1432"
                     /db_xref="GeneID:144114756"
ORIGIN      
        1 mrvpdhaqsf yigsrrqgak avtdsqsggg alplaqanas sfttevlaye ngygrrgkge
       61 ciasrrkttv vdsqsagalp laqanassft tevlaslplv aewtgvrewi rqanassftt
      121 evlayengyg rrgkgecias rrkttvvdsq sagalplaqa nassfttevl aslplvaewt
      181 gvrewirqan assfttevla yengygrrgk geciasrrkt tvvdsqsaga lplaqanass
      241 fttevlayen gygrrgkgec iasrrkttvv dsqsagalpl aqanassftt evlaslplva
      301 ewtgvrewir qrrpmparlr rrswrhypls qsglayengy grrgkgecia srrkttvvds
      361 qsagalplaq anassfttev laslplvaew tgvrewmrev rqr
//
LOCUS       XP_077504822             296 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114757 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504822
VERSION     XP_077504822.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648696.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..296
                     /product="uncharacterized protein LOC144114757 isoform X1"
                     /calculated_mol_wt=33617
     CDS             1..296
                     /gene="LOC144114757"
                     /coded_by="XM_077648696.1:231..1121"
                     /db_xref="GeneID:144114757"
ORIGIN      
        1 mhrvpeendr ggqpqrrhpp snagqcqpft tevlaslplv aewtgvrewi reanasrlrr
       61 rswrhypwsq sglayengyg rpmpavndgg pgvttpghrv dwrtrmdtgg eakanasrlr
      121 rrswrhypws qsglayengy ggagqcqlvy nggpgattpg rrvdcrtrmd tggeakanas
      181 sfttevlasl plvaewtgvr ewirqanass fttevlaslp lvsewtgvre wirevrqrpm
      241 parlrrrswr hypwsqsgla yengyrrpmp arlrrrswrh yplsesglay engygr
//
LOCUS       XP_077504823             296 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114757 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504823
VERSION     XP_077504823.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648697.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..296
                     /product="uncharacterized protein LOC144114757 isoform X1"
                     /calculated_mol_wt=33617
     CDS             1..296
                     /gene="LOC144114757"
                     /coded_by="XM_077648697.1:220..1110"
                     /db_xref="GeneID:144114757"
ORIGIN      
        1 mhrvpeendr ggqpqrrhpp snagqcqpft tevlaslplv aewtgvrewi reanasrlrr
       61 rswrhypwsq sglayengyg rpmpavndgg pgvttpghrv dwrtrmdtgg eakanasrlr
      121 rrswrhypws qsglayengy ggagqcqlvy nggpgattpg rrvdcrtrmd tggeakanas
      181 sfttevlasl plvaewtgvr ewirqanass fttevlaslp lvsewtgvre wirevrqrpm
      241 parlrrrswr hypwsqsgla yengyrrpmp arlrrrswrh yplsesglay engygr
//
LOCUS       XP_077504824             296 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114757 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504824
VERSION     XP_077504824.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648698.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..296
                     /product="uncharacterized protein LOC144114757 isoform X1"
                     /calculated_mol_wt=33617
     CDS             1..296
                     /gene="LOC144114757"
                     /coded_by="XM_077648698.1:137..1027"
                     /db_xref="GeneID:144114757"
ORIGIN      
        1 mhrvpeendr ggqpqrrhpp snagqcqpft tevlaslplv aewtgvrewi reanasrlrr
       61 rswrhypwsq sglayengyg rpmpavndgg pgvttpghrv dwrtrmdtgg eakanasrlr
      121 rrswrhypws qsglayengy ggagqcqlvy nggpgattpg rrvdcrtrmd tggeakanas
      181 sfttevlasl plvaewtgvr ewirqanass fttevlaslp lvsewtgvre wirevrqrpm
      241 parlrrrswr hypwsqsgla yengyrrpmp arlrrrswrh yplsesglay engygr
//
LOCUS       XP_077504825             270 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114757 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504825
VERSION     XP_077504825.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648699.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..270
                     /product="uncharacterized protein LOC144114757 isoform X2"
                     /calculated_mol_wt=30456
     CDS             1..270
                     /gene="LOC144114757"
                     /coded_by="XM_077648699.1:221..1033"
                     /db_xref="GeneID:144114757"
ORIGIN      
        1 mrvpdhaqsf yigsrrqgrw wtarataapc pslvrhegla lsafnrgwig eprkaqanas
       61 sfttevlssl plvagwtgvr ewirevrqrp mpavydggpg vttpgrrvdw rtrmdtggqc
      121 qlvynggpga ttpgrrvdcr trmdtggeak anassfttev laslplvaew tgvrewirqa
      181 nassfttevl aslplvsewt gvrewirevr qrpmparlrr rswrhypwsq sglayengyr
      241 rpmparlrrr swrhyplses glayengygr
//
LOCUS       XP_077504826             268 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114757 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504826
VERSION     XP_077504826.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648700.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..268
                     /product="uncharacterized protein LOC144114757 isoform X3"
                     /calculated_mol_wt=30228
     CDS             1..268
                     /gene="LOC144114757"
                     /coded_by="XM_077648700.1:221..1027"
                     /db_xref="GeneID:144114757"
ORIGIN      
        1 mrvpdhaqsf yigsrrqgrw wtarataapc pslvrhegla lsafnrgwig eprkaqanas
       61 sfttevlssl plvagwtgvr ewirerrpmp avydggpgvt tpgrrvdwrt rmdtggqcql
      121 vynggpgatt pgrrvdcrtr mdtggeakan assfttevla slplvaewtg vrewirqana
      181 ssfttevlas lplvsewtgv rewirevrqr pmparlrrrs wrhypwsqsg layengyrrp
      241 mparlrrrsw rhyplsesgl ayengygr
//
LOCUS       XP_077504827             267 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114757 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077504827
VERSION     XP_077504827.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648701.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..267
                     /product="uncharacterized protein LOC144114757 isoform X4"
                     /calculated_mol_wt=30158
     CDS             1..267
                     /gene="LOC144114757"
                     /coded_by="XM_077648701.1:231..1034"
                     /db_xref="GeneID:144114757"
ORIGIN      
        1 mhrvpeendr ggqpqrrhpp snagqcqpft tevlaslplv aewtgvrewi reanasrlrr
       61 rswrhypwsq sglayengyg rpmpavndgg pgvttpghrv dwrtrmdtgg eakanasrlr
      121 rrswrhypws qsglayengy ggagqcqlvy nggpgattpg rrvdcrtrmd tggeakanas
      181 sfttevlasl plvaewtgvr ewirqanass fttevlaslp lvsewtgvre wirevrqrpm
      241 parlrrrswr hypwsqsgla yengyrr
//
LOCUS       XP_077504828             245 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114757 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077504828
VERSION     XP_077504828.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648702.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..245
                     /product="uncharacterized protein LOC144114757 isoform X5"
                     /calculated_mol_wt=27471
     CDS             1..245
                     /gene="LOC144114757"
                     /coded_by="XM_077648702.1:84..821"
                     /db_xref="GeneID:144114757"
ORIGIN      
        1 mpavydggpg vttpgrrvdw rmrmdtggea kanassftte vlsslplvag wtgvrewire
       61 vrqrpmpavy dggpgvttpg rrvdwrtrmd tggqcqlvyn ggpgattpgr rvdcrtrmdt
      121 ggeakanass fttevlaslp lvaewtgvre wirqanassf ttevlaslpl vsewtgvrew
      181 irevrqrpmp arlrrrswrh ypwsqsglay engyrrpmpa rlrrrswrhy plsesglaye
      241 ngygr
//
LOCUS       XP_077504829             238 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114757 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077504829
VERSION     XP_077504829.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648703.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..238
                     /product="uncharacterized protein LOC144114757 isoform X6"
                     /calculated_mol_wt=27202
     CDS             1..238
                     /gene="LOC144114757"
                     /coded_by="XM_077648703.1:36..752"
                     /db_xref="GeneID:144114757"
ORIGIN      
        1 mparlrrrsw rhypwsqsgl ayengygrpm parlrrrscr hcpwslggla yengygrpmp
       61 avydggpgvt tpgrrvdwrt rmdtggqcql vynggpgatt pgrrvdcrtr mdtggeakan
      121 assfttevla slplvaewtg vrewirqana ssfttevlas lplvsewtgv rewirevrqr
      181 pmparlrrrs wrhypwsqsg layengyrrp mparlrrrsw rhyplsesgl ayengygr
//
LOCUS       XP_077504830             237 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114757 isoform X7 [Amblyomma
            americanum].
ACCESSION   XP_077504830
VERSION     XP_077504830.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648704.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..237
                     /product="uncharacterized protein LOC144114757 isoform X7"
                     /calculated_mol_wt=26455
     CDS             1..237
                     /gene="LOC144114757"
                     /coded_by="XM_077648704.1:231..944"
                     /db_xref="GeneID:144114757"
ORIGIN      
        1 mhrvpeendr ggqpqrrhpp snagqcqpft tevlaslplv aewtgvrewi reanasrlrr
       61 rswrhypwsq sglayengyg rpmpavndgg pgvttpghrv dwrtrmdtgg eakgiparll
      121 rrswrhyrwl qsalayengy grlskcayva slgtkavvds qsgdgalspa qanarsfamd
      181 silsepliad rlverewipw fvkarvtnvs fashyassal nrqdlsnier ttcvhst
//
LOCUS       XP_077504831             237 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114757 isoform X8 [Amblyomma
            americanum].
ACCESSION   XP_077504831
VERSION     XP_077504831.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648705.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..237
                     /product="uncharacterized protein LOC144114757 isoform X8"
                     /calculated_mol_wt=26867
     CDS             1..237
                     /gene="LOC144114757"
                     /coded_by="XM_077648705.1:181..894"
                     /db_xref="GeneID:144114757"
ORIGIN      
        1 mhrvpeendr ggqperrhpp snagqcqplt tevlasllqv tewtgvrewi rgvrqrpmpa
       61 vydggpgvtt pgrrvdwrtr mdtggqcqlv ynggpgattp grrvdcrtrm dtggeakana
      121 ssfttevlas lplvaewtgv rewirqanas sfttevlasl plvsewtgvr ewirevrqrp
      181 mparlrrrsw rhypwsqsgl ayengyrrpm parlrrrswr hyplsesgla yengygr
//
LOCUS       XP_077504832             220 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114757 isoform X9 [Amblyomma
            americanum].
ACCESSION   XP_077504832
VERSION     XP_077504832.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648706.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..220
                     /product="uncharacterized protein LOC144114757 isoform X9"
                     /calculated_mol_wt=24728
     CDS             1..220
                     /gene="LOC144114757"
                     /coded_by="XM_077648706.1:707..1369"
                     /db_xref="GeneID:144114757"
ORIGIN      
        1 mdtgaqanas sfttevlssl plvagwtgvr ewirevrqrp mpavydggpg vttpgrrvdw
       61 rtrmdtggqc qlvynggpga ttpgrrvdcr trmdtggeak anassfttev laslplvaew
      121 tgvrewirqa nassfttevl aslplvsewt gvrewirevr qrpmparlrr rswrhypwsq
      181 sglayengyr rpmparlrrr swrhyplses glayengygr
//
LOCUS       XP_077504833             218 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114757 isoform X10 [Amblyomma
            americanum].
ACCESSION   XP_077504833
VERSION     XP_077504833.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648707.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..218
                     /product="uncharacterized protein LOC144114757 isoform
                     X10"
                     /calculated_mol_wt=24670
     CDS             1..218
                     /gene="LOC144114757"
                     /coded_by="XM_077648707.1:707..1363"
                     /db_xref="GeneID:144114757"
ORIGIN      
        1 mdtggqcqlv ydggpvvtal grwvdwrtrm dtgaqanasr lrrrswrhyp wsqsglayen
       61 gyggagqcql vynggpgatt pgrrvdcrtr mdtggeakan assfttevla slplvaewtg
      121 vrewirqana ssfttevlas lplvsewtgv rewirevrqr pmparlrrrs wrhypwsqsg
      181 layengyrrp mparlrrrsw rhyplsesgl ayengygr
//
LOCUS       XP_077504834             540 aa            linear   INV 25-NOV-2025
DEFINITION  medium-chain acyl-CoA ligase ACSF2, mitochondrial-like [Amblyomma
            americanum].
ACCESSION   XP_077504834
VERSION     XP_077504834.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648708.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..540
                     /product="medium-chain acyl-CoA ligase ACSF2,
                     mitochondrial-like"
                     /calculated_mol_wt=59532
     Region          1..540
                     /region_name="PRK08315"
                     /note="AMP-binding domain protein; Validated"
                     /db_xref="CDD:236236"
     CDS             1..540
                     /gene="LOC144114759"
                     /coded_by="XM_077648708.1:386..2008"
                     /db_xref="GeneID:144114759"
ORIGIN      
        1 mrlsgetfgq hidaaaakwg devgwvfqqe nqhitfanyk ddvdrvckgl lsagftkgdt
       61 iaiwspntyn wvvlygamak agiisacvhp ayttaefekv mtkvrfagif ipesfkvlky
      121 yetlcamipe lkdsnpgqln ckkypflktv ivdsdkallg cinwqeilqg gnadiaeaer
      181 rvgmddpvti vftsgttgtp kaamlshhgf vnnsimydha apdlqhtvlc splpffhvfg
      241 mttvlgltvv rgncsvvpca gydtkeilks ihtyrcsqva gaptmitdmi nhadfhkfdl
      301 ssltkvvmgg navtpemrkl areklkahvw vgygatettt vstlltekdp eskqsttvgk
      361 pvghvevkia dpatgketai nepgevwvrg hniflgyhnd eektkevkap cgwyktgdlg
      421 kmdedgylsi igrlkdmiir ggeniypiei eevlathpav nechvigvpd srlseqvcaw
      481 vvlkpnakvt deelqqyckg rlshfkipky flyeadypkt aigkaqknkm reiaakklgl
//
LOCUS       XP_077504835             216 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger protein 346-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504835
VERSION     XP_077504835.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648709.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..216
                     /product="zinc finger protein 346-like isoform X1"
                     /calculated_mol_wt=23266
     Region          3..36
                     /region_name="ZnF_U1"
                     /note="U1-like zinc finger; smart00451"
                     /db_xref="CDD:197732"
     Region          8..30
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Site            order(15..17,19..20,24,29,86,89..93,95..96)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275371"
     Region          79..103
                     /region_name="zf-met"
                     /note="Zinc-finger of C2H2 type; pfam12874"
                     /db_xref="CDD:463736"
     Region          81..103
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     CDS             1..216
                     /gene="LOC144114762"
                     /coded_by="XM_077648709.1:1421..2071"
                     /db_xref="GeneID:144114762"
ORIGIN      
        1 mastgyscql csmqfngpvp yndhlrgakh hkkkvatqgy vealseggrp aaespmdida
       61 saasvgspti lapnpllpfv cklcnvamsc qaalishnsg kkhmkalyte etmrqvsvyg
      121 tifagpvpeq ahnqtamspt apaclpqqdq preheedvdl schycgivlf ksieyklehl
      181 etdahcnkkm qvigqlggtl qqssivpnvv qvikhp
//
LOCUS       XP_077504836             204 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114762 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504836
VERSION     XP_077504836.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648710.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..204
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..204
                     /product="uncharacterized protein LOC144114762 isoform X2"
                     /calculated_mol_wt=22033
     Region          67..91
                     /region_name="zf-met"
                     /note="Zinc-finger of C2H2 type; pfam12874"
                     /db_xref="CDD:463736"
     CDS             1..204
                     /gene="LOC144114762"
                     /coded_by="XM_077648710.1:1020..1634"
                     /db_xref="GeneID:144114762"
ORIGIN      
        1 mqfngpvpyn dhlrgakhhk kkvatqgyve alseggrpaa espmdidasa asvgsptila
       61 pnpllpfvck lcnvamscqa alishnsgkk hmkalyteet mrqvsvygti fagpvpeqah
      121 nqtamsptap aclpqqdqpr eheedvdlsc hycgivlfks ieyklehlet dahcnkkmqv
      181 igqlggtlqq ssivpnvvqv ikhp
//
LOCUS       XP_077504837             204 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114762 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504837
VERSION     XP_077504837.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648711.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..204
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..204
                     /product="uncharacterized protein LOC144114762 isoform X2"
                     /calculated_mol_wt=22033
     Region          67..91
                     /region_name="zf-met"
                     /note="Zinc-finger of C2H2 type; pfam12874"
                     /db_xref="CDD:463736"
     CDS             1..204
                     /gene="LOC144114762"
                     /coded_by="XM_077648711.1:283..897"
                     /db_xref="GeneID:144114762"
ORIGIN      
        1 mqfngpvpyn dhlrgakhhk kkvatqgyve alseggrpaa espmdidasa asvgsptila
       61 pnpllpfvck lcnvamscqa alishnsgkk hmkalyteet mrqvsvygti fagpvpeqah
      121 nqtamsptap aclpqqdqpr eheedvdlsc hycgivlfks ieyklehlet dahcnkkmqv
      181 igqlggtlqq ssivpnvvqv ikhp
//
LOCUS       XP_077504838             194 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114763 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504838
VERSION     XP_077504838.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648712.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..194
                     /product="uncharacterized protein LOC144114763 isoform X1"
                     /calculated_mol_wt=19945
     CDS             1..194
                     /gene="LOC144114763"
                     /coded_by="XM_077648712.1:2656..3240"
                     /db_xref="GeneID:144114763"
ORIGIN      
        1 mlgqpkqksk eedarkkrdr hktgggsaqc tvsaqseqvi avashimtrv gnqtdsdggi
       61 dlppvaslpv irllqpmvdg agneefyyae ddwepplvde pqspaaavgk sgatpeaieq
      121 esnlffpavq padasstaaa gcssatvasg aaagpttaea vatgdagrap rgrmallers
      181 ladendyiaa llre
//
LOCUS       XP_077504839              95 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114763 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504839
VERSION     XP_077504839.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648713.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..95
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..95
                     /product="uncharacterized protein LOC144114763 isoform X2"
                     /calculated_mol_wt=10010
     CDS             1..95
                     /gene="LOC144114763"
                     /coded_by="XM_077648713.1:2656..2943"
                     /db_xref="GeneID:144114763"
ORIGIN      
        1 mlgqpkqksk eedarkkrdr hktgggsaqc tvsaqseqvi avashimtrv gnqtdsdggi
       61 dlppvaslpv irllqpmvdg agneefyyad kgcig
//
LOCUS       XP_077504840             137 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114764 [Amblyomma americanum].
ACCESSION   XP_077504840
VERSION     XP_077504840.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648714.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..137
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..137
                     /product="uncharacterized protein LOC144114764"
                     /calculated_mol_wt=14879
     Region          <19..76
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..137
                     /gene="LOC144114764"
                     /coded_by="XM_077648714.1:518..931"
                     /db_xref="GeneID:144114764"
ORIGIN      
        1 msastarvaq irmstetaka ispsmcryqk ahiktrnvve rtfgvwkhlf pcldmglqhk
       61 pkqaaviita caalqnfacl mkepqppies apiqdappar tsttaarrpe hlppvdavsd
      121 slsgmqaqrv liqksft
//
LOCUS       XP_077504841            1052 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger MIZ domain-containing protein tonalli isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077504841
VERSION     XP_077504841.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648715.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1052
                     /product="zinc finger MIZ domain-containing protein
                     tonalli isoform X1"
                     /calculated_mol_wt=109003
     Region          20..>413
                     /region_name="Med15"
                     /note="ARC105 or Med15 subunit of Mediator complex
                     non-fungal; pfam09606"
                     /db_xref="CDD:312941"
     Region          <266..>400
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     Region          619..682
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Region          <620..774
                     /region_name="COG5222"
                     /note="Uncharacterized conserved protein, contains RING
                     Zn-finger [General function prediction only]"
                     /db_xref="CDD:227547"
     Site            order(624,627,640,642,647,650,663,666)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     CDS             1..1052
                     /gene="tna"
                     /coded_by="XM_077648715.1:96..3254"
                     /db_xref="GeneID:144114765"
ORIGIN      
        1 mcsitpmtql eivadvycdg qalsgsdagg msglgwqsgg gpvsqqqqls vvttvwgvts
       61 ttqsaapyap gppqakggpy apagypsgsp lgptkgyspv ppsrqppnay agygqnpagm
      121 aggmpgamag aghgtagefq tgpsalstaa lvaaatatat atasvvalqe qrqqevmggq
      181 ygqmgghqqf pgggygvppq rgpphggaag gqmgglmgpa mgplgasklg gaggplygqq
      241 qqqpqqqqqq ppprgrpypn pqqylahkrq qqqypagpmq ggymgapqqq ygpqypgaas
      301 gpfpkqqsgg yggapapqga laayhqgspl rgrppgqamp tpgaypggaq ggqyyqqpmg
      361 pqaqyeqqfm qgsqyaphsf qrsmnyqhsp ipgnptpplt pasgmppyls psqdtkpvfp
      421 dikpplpiqk ddelrltfpv kdgiilapfr lehnlavsnh vfhlkpsvhe tlvwrsdlel
      481 qlkcfhhedr qmhtnwpasv qvsvnatpls idrgesktsh rplylkevcq agrntvqitv
      541 tacccshlfl lqlvhrptvr svlqgllrkr llpaehsigk ikrnfsvgps pqgpntapgq
      601 npsggagpve dgveqtaikv qlkcpitfkr itlpargqec khiqcfdles ylqlncergs
      661 wrcpvcskta ileglevdqy mwgiltnlsn sdveevtida taswkpvtik sikdehdgte
      721 scsggkrlka mspssmtmpt tsswemgqgl spypalpppd mqsivngpnm angpvmsggp
      781 vmptgagmpn gpvmaggpnm pngpvmgngs gmpngmgygs rnggfdfqsq asdfgsplsh
      841 lndsvasldh laamekslnh heqqmmpmsn hhqdppapqg asssrhpgsg gpasqntgsn
      901 apgtpstsgg gntpassqsq appqqqqppl svgsngphtp htphtphtpg gppsvppqgl
      961 egsggpesla pdlselsfdp aavidgegqg qeglnllpes cvdamellsy ldppdlgggt
     1021 gstgsshagq asssatagst panddllalf ep
//
LOCUS       XP_077504842            1053 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger MIZ domain-containing protein tonalli isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077504842
VERSION     XP_077504842.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648716.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1053
                     /product="zinc finger MIZ domain-containing protein
                     tonalli isoform X2"
                     /calculated_mol_wt=109131
     Region          20..>414
                     /region_name="Med15"
                     /note="ARC105 or Med15 subunit of Mediator complex
                     non-fungal; pfam09606"
                     /db_xref="CDD:312941"
     Region          <266..>393
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     Region          620..683
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Region          <621..775
                     /region_name="COG5222"
                     /note="Uncharacterized conserved protein, contains RING
                     Zn-finger [General function prediction only]"
                     /db_xref="CDD:227547"
     Site            order(625,628,641,643,648,651,664,667)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     CDS             1..1053
                     /gene="tna"
                     /coded_by="XM_077648716.1:568..3729"
                     /db_xref="GeneID:144114765"
ORIGIN      
        1 mcsitpmtql eivadvycdg qalsgsdagg msglgwqsgg gpvsqqqqls vvttvwgvts
       61 ttqsaapyap gppqakggpy apagypsgsp lgptkgyspv ppsrqppnay agygqnpagm
      121 aggmpgamag aghgtagefq tgpsalstaa lvaaatatat atasvvalqe qrqqevmggq
      181 ygqmgghqqf pgggygvppq rgpphggaag gqmgglmgpa mgplgasklg gaggplygqq
      241 qqqpqqqqqq ppprgrpypn pqqylahkrq qqqypagpmq ggymgapqqq ygpqypgaas
      301 gpfpkqqsgg yggapapqga laayhqgspl rgrppgqamp tpgaypggaq ggqyyqqpmg
      361 pqaqyeqqfm qgsqyaphsf qqrsmnyqhs pipgnptppl tpasgmppyl spsqdtkpvf
      421 pdikpplpiq kddelrltfp vkdgiilapf rlehnlavsn hvfhlkpsvh etlvwrsdle
      481 lqlkcfhhed rqmhtnwpas vqvsvnatpl sidrgeskts hrplylkevc qagrntvqit
      541 vtacccshlf llqlvhrptv rsvlqgllrk rllpaehsig kikrnfsvgp spqgpntapg
      601 qnpsggagpv edgveqtaik vqlkcpitfk ritlpargqe ckhiqcfdle sylqlncerg
      661 swrcpvcskt aileglevdq ymwgiltnls nsdveevtid ataswkpvti ksikdehdgt
      721 escsggkrlk amspssmtmp ttsswemgqg lspypalppp dmqsivngpn mangpvmsgg
      781 pvmptgagmp ngpvmaggpn mpngpvmgng sgmpngmgyg srnggfdfqs qasdfgspls
      841 hlndsvasld hlaameksln hheqqmmpms nhhqdppapq gasssrhpgs ggpasqntgs
      901 napgtpstsg ggntpassqs qappqqqqpp lsvgsngpht phtphtphtp ggppsvppqg
      961 legsggpesl apdlselsfd paavidgegq gqeglnllpe scvdamells yldppdlggg
     1021 tgstgsshag qasssatags tpanddllal fep
//
LOCUS       XP_077504843            1023 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger MIZ domain-containing protein tonalli isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077504843
VERSION     XP_077504843.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648717.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1023
                     /product="zinc finger MIZ domain-containing protein
                     tonalli isoform X3"
                     /calculated_mol_wt=106116
     Region          2..>384
                     /region_name="Med15"
                     /note="ARC105 or Med15 subunit of Mediator complex
                     non-fungal; pfam09606"
                     /db_xref="CDD:312941"
     Region          <236..>363
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     Region          590..653
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Region          <591..745
                     /region_name="COG5222"
                     /note="Uncharacterized conserved protein, contains RING
                     Zn-finger [General function prediction only]"
                     /db_xref="CDD:227547"
     Site            order(595,598,611,613,618,621,634,637)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     CDS             1..1023
                     /gene="tna"
                     /coded_by="XM_077648717.1:274..3345"
                     /db_xref="GeneID:144114765"
ORIGIN      
        1 msglgwqsgg gpvsqqqqls vvttvwgvts ttqsaapyap gppqakggpy apagypsgsp
       61 lgptkgyspv ppsrqppnay agygqnpagm aggmpgamag aghgtagefq tgpsalstaa
      121 lvaaatatat atasvvalqe qrqqevmggq ygqmgghqqf pgggygvppq rgpphggaag
      181 gqmgglmgpa mgplgasklg gaggplygqq qqqpqqqqqq ppprgrpypn pqqylahkrq
      241 qqqypagpmq ggymgapqqq ygpqypgaas gpfpkqqsgg yggapapqga laayhqgspl
      301 rgrppgqamp tpgaypggaq ggqyyqqpmg pqaqyeqqfm qgsqyaphsf qqrsmnyqhs
      361 pipgnptppl tpasgmppyl spsqdtkpvf pdikpplpiq kddelrltfp vkdgiilapf
      421 rlehnlavsn hvfhlkpsvh etlvwrsdle lqlkcfhhed rqmhtnwpas vqvsvnatpl
      481 sidrgeskts hrplylkevc qagrntvqit vtacccshlf llqlvhrptv rsvlqgllrk
      541 rllpaehsig kikrnfsvgp spqgpntapg qnpsggagpv edgveqtaik vqlkcpitfk
      601 ritlpargqe ckhiqcfdle sylqlncerg swrcpvcskt aileglevdq ymwgiltnls
      661 nsdveevtid ataswkpvti ksikdehdgt escsggkrlk amspssmtmp ttsswemgqg
      721 lspypalppp dmqsivngpn mangpvmsgg pvmptgagmp ngpvmaggpn mpngpvmgng
      781 sgmpngmgyg srnggfdfqs qasdfgspls hlndsvasld hlaameksln hheqqmmpms
      841 nhhqdppapq gasssrhpgs ggpasqntgs napgtpstsg ggntpassqs qappqqqqpp
      901 lsvgsngpht phtphtphtp ggppsvppqg legsggpesl apdlselsfd paavidgegq
      961 gqeglnllpe scvdamells yldppdlggg tgstgsshag qasssatags tpanddllal
     1021 fep
//
LOCUS       XP_077504844             674 aa            linear   INV 25-NOV-2025
DEFINITION  visceral mesodermal armadillo-repeats isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504844
VERSION     XP_077504844.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648718.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..674
                     /product="visceral mesodermal armadillo-repeats isoform
                     X1"
                     /calculated_mol_wt=73555
     Region          94..133
                     /region_name="Arm"
                     /note="Armadillo/beta-catenin-like repeat; pfam00514"
                     /db_xref="CDD:425727"
     Region          98..134
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     CDS             1..674
                     /gene="vimar"
                     /coded_by="XM_077648718.1:139..2163"
                     /db_xref="GeneID:144114766"
ORIGIN      
        1 mdniteilnd lklgagqdpk ascadlslrl ksllallsad ekdpkseasr qevcqrlvks
       61 ifvsstkdll kvtstisedt lallvqviae aakseplrkq ftdaavvepl lglvscknya
      121 vvlqacralg nicydnddgr dlvrskqgve qllqllqnvl evdsaprdlh tvvsgcllnl
      181 tdmqeiqeda lrlgvidill kylqkysdve dvvmhcilvl ngiadsddgr krltekhtls
      241 lllsnldkvf seevtevlle lfgnlaesde vkedlvelgf ceklievlkr fqsapgvpps
      301 eekqsvvktv cdltvlvltg dasmrhvyqn gsgpiykatm awlqadddnl rvagalsagn
      361 farkddhciq mvrdgvarrl ldlltehtgt dgdirlqhal laalrnlaip lenkpllael
      421 gavdkllsml avetfpvvfk llgtlrmlvd kqeavatklg lneqlikhlv twcntedhpg
      481 vkgesvrlla wiaknsasce akatlcrlga lphwvsmlss ehsvmqaegl laltlaltps
      541 ppsaaledlk ktdilpllrs llespeispe alqnavaltt alsnldsfrp llqaegysav
      601 ltelkdhsnq aiskaatqal qvlekklvss gaqsfttcee dlqhgsypwq lsvpfelnlk
      661 ffpisfvvlf plgk
//
LOCUS       XP_077504845             651 aa            linear   INV 25-NOV-2025
DEFINITION  visceral mesodermal armadillo-repeats isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504845
VERSION     XP_077504845.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648719.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..651
                     /product="visceral mesodermal armadillo-repeats isoform
                     X2"
                     /calculated_mol_wt=71121
     Region          94..133
                     /region_name="Arm"
                     /note="Armadillo/beta-catenin-like repeat; pfam00514"
                     /db_xref="CDD:425727"
     Region          98..134
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     CDS             1..651
                     /gene="vimar"
                     /coded_by="XM_077648719.1:134..2089"
                     /db_xref="GeneID:144114766"
ORIGIN      
        1 mdniteilnd lklgagqdpk ascadlslrl ksllallsad ekdpkseasr qevcqrlvks
       61 ifvsstkdll kvtstisedt lallvqviae aakseplrkq ftdaavvepl lglvscknya
      121 vvlqacralg nicydnddgr dlvrskqgve qllqllqnvl evdsaprdlh tvvsgcllnl
      181 tdmqeiqeda lrlgvidill kylqkysdve dvvmhcilvl ngiadsddgr krltekhtls
      241 lllsnldkvf seevtevlle lfgnlaesde vkedlvelgf ceklievlkr fqsapgvpps
      301 eekqsvvktv cdltvlvltg dasmrhvyqn gsgpiykatm awlqadddnl rvagalsagn
      361 farkddhciq mvrdgvarrl ldlltehtgt dgdirlqhal laalrnlaip lenkpllael
      421 gavdkllsml avetfpvvfk llgtlrmlvd kqeavatklg lneqlikhlv twcntedhpg
      481 vkgesvrlla wiaknsasce akatlcrlga lphwvsmlss ehsvmqaegl laltlaltps
      541 ppsaaledlk ktdilpllrs llespeispe alqnavaltt alsnldgsaa whwapgginr
      601 dtmwtwgvlr lafgllylfs iphrchamkp tltdhgpslv rfdaeifaet n
//
LOCUS       XP_077504846             537 aa            linear   INV 25-NOV-2025
DEFINITION  DNA-dependent metalloprotease SPRTN-like [Amblyomma americanum].
ACCESSION   XP_077504846
VERSION     XP_077504846.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648720.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..537
                     /product="DNA-dependent metalloprotease SPRTN-like"
                     /calculated_mol_wt=59306
     Region          69..238
                     /region_name="SprT"
                     /note="SprT homologues; smart00731"
                     /db_xref="CDD:214794"
     Region          510..533
                     /region_name="ZnF_Rad18"
                     /note="Rad18-like CCHC zinc finger; smart00734"
                     /db_xref="CDD:128973"
     CDS             1..537
                     /gene="LOC144114769"
                     /coded_by="XM_077648720.1:216..1829"
                     /db_xref="GeneID:144114769"
ORIGIN      
        1 msmasededf llslqlalqi teeheyhesl lklnepsiek pagtsvvshs lrkdlsvvde
       61 fwelhdpnpd vhrlfvefne ayfygkldhv avcwswrmtl caglcqyegk gglctvklse
      121 pllklrprkd lvetllhemi haylfvtnnn kdhnahgpef hkhmdrinka sganitvfhn
      181 fheevdnyrt hwwrcngpcq hrlpffgivk ramnrppsek dpwwadhqrk cggtftkire
      241 pdpkgskgkr kdgtlpvkna akkpklmdga dirtllipph sfksavsvpd sqghqgtrpk
      301 hvssfpgimt klpgnvhtls hrasdmqqpq vapytgeght lggcstksrl leegasrsvs
      361 ivesscvlgg vssqihnfea neatsgssav srpgqslnkc vtltevkpap qkpspltqpt
      421 lkdflgqktq paertsrkss aakmkfirdl lssdsesddd cviiaadvtv keqgkrsgkl
      481 deastssssl lthtdvgata nvsagnvlal iecpvcgqlm qeqfinshld mcliqdd
//
LOCUS       XP_077504847             461 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: acyl-coenzyme A diphosphatase NUDT19-like
            [Amblyomma americanum].
ACCESSION   XP_077504847
VERSION     XP_077504847.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648721.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..461
                     /product="LOW QUALITY PROTEIN: acyl-coenzyme A
                     diphosphatase NUDT19-like"
                     /calculated_mol_wt=51206
     Region          79..348
                     /region_name="NUDIX_AcylCoAdiphos_Nudt19"
                     /note="Nucleoside diphosphate-linked moiety X)) motif 19;
                     cd18870"
                     /db_xref="CDD:467582"
     CDS             1..461
                     /gene="LOC144114770"
                     /coded_by="XM_077648721.1:31..1416"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 1 base in 1 codon"
                     /db_xref="GeneID:144114770"
ORIGIN      
        1 maaaairvvw hrcqfagspl yrgrfalgnl gspeaqssly flpacsagla amqarhsshl
       61 rgskksgtes amsappwkea aslivvsrap lrdvvgkdla tammatvwnn qtervsrcdy
      121 rvlmvkrssl ssfmanayvy pgghcepcdf spdwwevfaa agaskeallr dvcnrakgpr
      181 ppmitkpmtl shnqlkpedh lpgdlayris airetfeetg vllltqaaps fsetlvtesl
      241 segidttqwr qrlredpvnl lrmcrenrlc pnvwalheww dwltpisvgh rrydtmfyic
      301 cmdkqpqvvl dqsevitlkw ctpegmlqdy stgavflapp qvyetsrlln vtsfaelsqf
      361 acrrgaegce qwmpviasas dgavsllpgd dlypeepdyf grgpgpeypf sleelrkrcq
      421 nlhrmevqgp lctaysniep prghlqpqtf xgtasmvpsm l
//
LOCUS       XP_077504849             353 aa            linear   INV 25-NOV-2025
DEFINITION  N-terminal EF-hand calcium-binding protein 1-like [Amblyomma
            americanum].
ACCESSION   XP_077504849
VERSION     XP_077504849.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648723.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..353
                     /product="N-terminal EF-hand calcium-binding protein
                     1-like"
                     /calculated_mol_wt=40192
     Region          <21..76
                     /region_name="FRQ1"
                     /note="Ca2+-binding protein, EF-hand superfamily [Signal
                     transduction mechanisms]; COG5126"
                     /db_xref="CDD:444056"
     Region          257..341
                     /region_name="YgiN"
                     /note="Quinol monooxygenase YgiN [Energy production and
                     conversion]; COG1359"
                     /db_xref="CDD:440970"
     CDS             1..353
                     /gene="LOC144114771"
                     /coded_by="XM_077648723.1:151..1212"
                     /db_xref="GeneID:144114771"
ORIGIN      
        1 madttspfln lqkgtsvlln vlnradtngd gvlakeefln ffwdavlted elcelfeein
       61 vhktgfidag elssfvwrhm gnfptllasl edlsvavtkg lqqmekfyds ssiqdqfvsr
      121 flvretafql skltaameas qkrlqeegip lidgsdsedd dgkalrkpnc nfqaalgsqs
      181 lqvykpcpsr llpvrirkqm vtqtslhlee ssqdlsaqik kleaivekla nqpkfqkpqe
      241 delditeenl lflvqqtmpv deeqmedyke slktyaekta atagclfvsv rfykdintya
      301 vyeiwesedt wnkhlrsniy ktlqhqniec lrkpeflntm eipgswwkaa sek
//
LOCUS       XP_077504850             653 aa            linear   INV 25-NOV-2025
DEFINITION  phosphoenolpyruvate carboxykinase, cytosolic [GTP]-like [Amblyomma
            americanum].
ACCESSION   XP_077504850
VERSION     XP_077504850.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648724.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..653
                     /product="phosphoenolpyruvate carboxykinase, cytosolic
                     [GTP]-like"
                     /calculated_mol_wt=72180
     Region          52..644
                     /region_name="PEPCK_GTP"
                     /note="Phosphoenolpyruvate carboxykinase (PEPCK), a
                     critical gluconeogenic enzyme, catalyzes the first
                     committed step in the diversion of tricarboxylic acid
                     cycle intermediates toward gluconeogenesis. It catalyzes
                     the reversible decarboxylation and...; cd00819"
                     /db_xref="CDD:238417"
     Site            order(112,261,263,269..270,290,312..318,336..337,359,361,
                     370,432,554,557,560)
                     /site_type="active"
                     /db_xref="CDD:238417"
     Site            order(112,261,263,269..270,359,432)
                     /site_type="other"
                     /note="substrate-binding site [chemical binding]"
                     /db_xref="CDD:238417"
     Site            order(270,290,317,336..337,359)
                     /site_type="metal-binding"
                     /note="metal-binding site [ion binding]"
                     /db_xref="CDD:238417"
     Site            order(313,315,463,543..544,554,557,560)
                     /site_type="other"
                     /note="GTP binding site [chemical binding]"
                     /db_xref="CDD:238417"
     CDS             1..653
                     /gene="LOC144114772"
                     /coded_by="XM_077648724.1:147..2108"
                     /db_xref="GeneID:144114772"
ORIGIN      
        1 mmdtsrppta paesapsspp ehkpaapvde dwlfkppavw kghdvtdlpk kvrhfveeqr
       61 rlcqpasvyv cdgsseenea iiafmiklgl avplkkhtnc ylvrtdprdv arvegrtcia
      121 tarqeetapi papgvkgilg nwiapeelrk ilsecipgcm kgrtmfvipf smcpvespls
      181 rlgvqvtdsp yvvasmrimt rmgakplaat kdkefvkcvh smgrplplke plvsnwpcnp
      241 ektiishiph dneivsfgsg yggnsllakk cfalrigcnl grregwlaeh mlilgvttps
      301 gnksyivgaf psacgktnla mitptlpgyr aecvgddiaw lyfdengimr avnpengffg
      361 vcpgtsmktn pnammtiqtn tiftnvaets dgdvwwegld lpppdvtiks wknepnwkpg
      421 dksktaahpn arfctpagqc piidpawedp kgvpvsaiif ggrrpegvpl vfeafnwnhg
      481 vflgacmrse ttaaaehkak vimhdpfgmr pffgynfgdy lthwlsfake egnqlpkiyh
      541 vnwfrkgadg kflwpgfgen srvldwiirr vdgevniare tflgyvptdd alnlsglsep
      601 vniaelmrvd kefwikecrd ikaffdeqvg rslppaiadq lamlkerimk sps
//
LOCUS       XP_077504851             141 aa            linear   INV 25-NOV-2025
DEFINITION  ragulator complex protein LAMTOR3-like [Amblyomma americanum].
ACCESSION   XP_077504851
VERSION     XP_077504851.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648725.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="ragulator complex protein LAMTOR3-like"
                     /calculated_mol_wt=15214
     Region          20..138
                     /region_name="MAPKK1_Int"
                     /note="Mitogen-activated protein kinase kinase 1
                     interacting; pfam08923"
                     /db_xref="CDD:462635"
     CDS             1..141
                     /gene="LOC144114773"
                     /coded_by="XM_077648725.1:19..444"
                     /db_xref="GeneID:144114773"
ORIGIN      
        1 mqrvcavacr vlyrvnrmad elkkhliqvi srvqglqaiv fadrdgvpli kaatdeapdh
       61 alrpgflatv gmamsqgnkl glgkcnkmis sygnyqvvsf nrhpvtitiv asssantgmi
      121 lnleeelntv sqtlrsvvev s
//
LOCUS       XP_077504852             142 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114774 [Amblyomma americanum].
ACCESSION   XP_077504852
VERSION     XP_077504852.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648726.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..142
                     /product="uncharacterized protein LOC144114774"
                     /calculated_mol_wt=14636
     CDS             1..142
                     /gene="LOC144114774"
                     /coded_by="XM_077648726.1:354..782"
                     /db_xref="GeneID:144114774"
ORIGIN      
        1 masgqyigee mgllelsgkl ltthrgdsds dnapeqwsvd kddaeglhrp ledpeaaeee
       61 npggvlvgsg leplgngass eavniahsgp tetcpeppkc aglmgllalc vgaslgfvav
      121 liafsayvyq trfsakyspa yp
//
LOCUS       XP_077504853             142 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114775 [Amblyomma americanum].
ACCESSION   XP_077504853
VERSION     XP_077504853.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648727.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..142
                     /product="uncharacterized protein LOC144114775"
                     /calculated_mol_wt=14754
     CDS             1..142
                     /gene="LOC144114775"
                     /coded_by="XM_077648727.1:321..749"
                     /db_xref="GeneID:144114775"
ORIGIN      
        1 masgqyigee malvelagkf lpthrgdsds ynapeqwsvd kddaeglhrp ledpaaaeee
       61 npggvlvgsg leplgngass eamniaqsgp metypeppkc aglmgllalc vgaslgfvav
      121 liafsayvyq trfsakyspa yp
//
LOCUS       XP_077504854             142 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114776 [Amblyomma americanum].
ACCESSION   XP_077504854
VERSION     XP_077504854.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648728.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..142
                     /product="uncharacterized protein LOC144114776"
                     /calculated_mol_wt=14754
     CDS             1..142
                     /gene="LOC144114776"
                     /coded_by="XM_077648728.1:321..749"
                     /db_xref="GeneID:144114776"
ORIGIN      
        1 masgqyigee malvelagkf lpthrgdsds ynapeqwsvd kddaeglhrp ledpaaaeee
       61 npggvlvgsg leplgngass eamniaqsgp metypeppkc aglmgllalc vgaslgfvav
      121 liafsayvyq trfsakyspa yp
//
LOCUS       XP_077504855             299 aa            linear   INV 25-NOV-2025
DEFINITION  neuropeptide SIFamide receptor-like [Amblyomma americanum].
ACCESSION   XP_077504855
VERSION     XP_077504855.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648729.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..299
                     /product="neuropeptide SIFamide receptor-like"
                     /calculated_mol_wt=33343
     Region          <1..224
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          8..30
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410628"
     Region          51..67
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410628"
     Region          97..121
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410628"
     Region          151..173
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410628"
     Region          192..217
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410628"
     CDS             1..299
                     /gene="LOC144114780"
                     /coded_by="XM_077648729.1:8..907"
                     /db_xref="GeneID:144114780"
ORIGIN      
        1 mlgwfmcktv sylqgvavsa sintlvaism drclaicypl kcqlstrsvr killviwsfs
       61 vaitfpwaly ftlqpihpsi pgvtlcveqw pdersstlyf ilahlvlcyi fplllivvcy
      121 gciwvkvwrr nipgeskqtd lmvqksklkv vkmmlvvvvi fvlswlplyi iftrlklgsa
      181 peegsvdwnl mliltpvaqw lgasnscinp vlyayfnrkf rrgfvaivks rsccgtlrep
      241 sslrgttirs aaggahrgls rseipecvah prasakkdtl ltngrtdaee khakvslva
//
LOCUS       XP_077504856             368 aa            linear   INV 25-NOV-2025
DEFINITION  erlin-1-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504856
VERSION     XP_077504856.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648730.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..368
                     /product="erlin-1-like isoform X1"
                     /calculated_mol_wt=41648
     Region          17..313
                     /region_name="SPFH_like_u3"
                     /note="Uncharacterized family; SPFH (stomatin, prohibitin,
                     flotillin, and HflK/C) superfamily; cd03406"
                     /db_xref="CDD:259804"
     CDS             1..368
                     /gene="LOC144114784"
                     /coded_by="XM_077648730.1:210..1316"
                     /db_xref="GeneID:144114784"
ORIGIN      
        1 mpnsgvaalt cafvlflvvn fslhkidegh vgvyyrggal lkqtsnpgfh mmvpfittyr
       61 siqvtlqtde vknvpcgtsg gvmiyfdrie vvnilspdse tslsvydmvk nytadydktl
      121 ifnkvhheln qfcsvhnlqe vyinlfdqid enlktalqrd lnvmapglfv qavrvtkpki
      181 petirrnyem meaektklli aeqkqkvvek daetdrkkai idaekvaqva kiqyqqrile
      241 qeslkkmsyi edemhfsrek mkadgefysk qklaeankll ltpeflelkr yeaiaannkv
      301 yfgkdipsmf inhcseqsqs daanlhsvts slggkeskvs ehskpmkklv vdqgstgakf
      361 yeearpgy
//
LOCUS       XP_077504857             368 aa            linear   INV 25-NOV-2025
DEFINITION  erlin-1-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504857
VERSION     XP_077504857.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648731.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..368
                     /product="erlin-1-like isoform X1"
                     /calculated_mol_wt=41648
     Region          17..313
                     /region_name="SPFH_like_u3"
                     /note="Uncharacterized family; SPFH (stomatin, prohibitin,
                     flotillin, and HflK/C) superfamily; cd03406"
                     /db_xref="CDD:259804"
     CDS             1..368
                     /gene="LOC144114784"
                     /coded_by="XM_077648731.1:166..1272"
                     /db_xref="GeneID:144114784"
ORIGIN      
        1 mpnsgvaalt cafvlflvvn fslhkidegh vgvyyrggal lkqtsnpgfh mmvpfittyr
       61 siqvtlqtde vknvpcgtsg gvmiyfdrie vvnilspdse tslsvydmvk nytadydktl
      121 ifnkvhheln qfcsvhnlqe vyinlfdqid enlktalqrd lnvmapglfv qavrvtkpki
      181 petirrnyem meaektklli aeqkqkvvek daetdrkkai idaekvaqva kiqyqqrile
      241 qeslkkmsyi edemhfsrek mkadgefysk qklaeankll ltpeflelkr yeaiaannkv
      301 yfgkdipsmf inhcseqsqs daanlhsvts slggkeskvs ehskpmkklv vdqgstgakf
      361 yeearpgy
//
LOCUS       XP_077504858             363 aa            linear   INV 25-NOV-2025
DEFINITION  erlin-1-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504858
VERSION     XP_077504858.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648732.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..363
                     /product="erlin-1-like isoform X2"
                     /calculated_mol_wt=41130
     Region          17..308
                     /region_name="SPFH_like_u3"
                     /note="Uncharacterized family; SPFH (stomatin, prohibitin,
                     flotillin, and HflK/C) superfamily; cd03406"
                     /db_xref="CDD:259804"
     CDS             1..363
                     /gene="LOC144114784"
                     /coded_by="XM_077648732.1:210..1301"
                     /db_xref="GeneID:144114784"
ORIGIN      
        1 mpnsgvaalt cafvlflvvn fslhkidegh vgvyyrggal lkqtsnpgfh mmvpfittyr
       61 siqvtlqtde vknvpcgtsg gvmiyfdrie vvnilspdsv ydmvknytad ydktlifnkv
      121 hhelnqfcsv hnlqevyinl fdqidenlkt alqrdlnvma pglfvqavrv tkpkipetir
      181 rnyemmeaek tklliaeqkq kvvekdaetd rkkaiidaek vaqvakiqyq qrileqeslk
      241 kmsyiedemh fsrekmkadg efyskqklae ankllltpef lelkryeaia annkvyfgkd
      301 ipsmfinhcs eqsqsdaanl hsvtsslggk eskvsehskp mkklvvdqgs tgakfyeear
      361 pgy
//
LOCUS       XP_077504859             878 aa            linear   INV 25-NOV-2025
DEFINITION  Z band alternatively spliced PDZ-motif protein 52 isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077504859
VERSION     XP_077504859.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648733.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..878
                     /product="Z band alternatively spliced PDZ-motif protein
                     52 isoform X1"
                     /calculated_mol_wt=91305
     Region          7..87
                     /region_name="PDZ_ZASP52-like"
                     /note="PDZ domain of Drosophila melanogaster PDZ and LIM
                     domain protein Zasp52 (also known as Zasp), and related
                     domains; cd23068"
                     /db_xref="CDD:467281"
     Site            order(17..23,35,38,67,71,74..75)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467281"
     Region          150..>212
                     /region_name="DUF4749"
                     /note="Domain of unknown function (DUF4749); pfam15936"
                     /db_xref="CDD:464948"
     Region          312..363
                     /region_name="LIM_ALP_like"
                     /note="The LIM domain of ALP (actinin-associated LIM
                     protein) family; cd09360"
                     /db_xref="CDD:188746"
     Site            order(312,315,332,335,338,341,359,362)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188746"
     Region          <414..689
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          704..757
                     /region_name="LIM1_Enigma_like_1"
                     /note="The first LIM domain of an Enigma subfamily with
                     unknown function; cd09455"
                     /db_xref="CDD:188839"
     Site            order(704,707,724,727,730,733,753,756)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188839"
     Region          765..816
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(765,768,785,788,791,794,812,815)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:259829"
     Region          824..877
                     /region_name="LIM3_Enigma_like_1"
                     /note="The third LIM domain of an Enigma subfamily with
                     unknown function; cd09461"
                     /db_xref="CDD:188845"
     Site            order(824,827,846,849,852,855,873,876)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188845"
     CDS             1..878
                     /gene="Zasp52"
                     /coded_by="XM_077648733.1:567..3203"
                     /db_xref="GeneID:144114785"
ORIGIN      
        1 mnesvitvkl qrgdgtswgf rlaggkdfgy plsiqrvnpg slaekgglqt gdalisiqgk
       61 stdqlrhkea qdaimragny lelqvtrgas mtwkpkvtpv gsmpkpgpms nggpapvtkt
      121 slaankqpyk pigtghntaa kpfggtmqav vhnqynspmp vysmnniadt lsaqtevlts
      181 gavginfmkg dkplnkdsav yrmvleedkh pkgdesdlvs vaetrtqsla frkllaalgd
      241 dddclrsshd vtdygsggss spalggpppa avtassptpa pmvnlrhvea pktapapppg
      301 qpnttvgapn tcadcgrliv gvfvrikdra lhaecfrcat cgtslknigy fnikdklycd
      361 ihakqaarhi apgsnleavp vppgghipqg vplapapapa pavvpvapra pagavspqgp
      421 lytppapkyt paaapytptq aapvhspvaa apladqpppp pdspgicyds pvamgrsdfr
      481 qapthspvag skpltpqsak ttppfggvtp lhpvtapvgd ahrpvpggak ftwppaqagg
      541 ptplssgvle snatpsvpty rpppgtqrva apstpkwggp sqapggpfrp sspsaspysp
      601 vsspassrpp sfvssspspl sgfggqpgvk vppptapkpv arapsakmgn ggpwgspapa
      661 lstgassapf tgpsglsqga gsrpapkrgr gkltqaiggk ipicsscgsp irgpfvtalg
      721 rnwcpehflc sngscrkslq digfveeqgk lycehcyecy mapicrkcgh rikgdclnal
      781 eqtwhpecfv ctycktafgn ssfyledgmp ycekdwnelf ttkcvgcgfp ieagdrwvea
      841 lnnnyhsqcf kcticqknle gqsffakggr pfckahar
//
LOCUS       XP_077504860             838 aa            linear   INV 25-NOV-2025
DEFINITION  Z band alternatively spliced PDZ-motif protein 52 isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077504860
VERSION     XP_077504860.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648734.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..838
                     /product="Z band alternatively spliced PDZ-motif protein
                     52 isoform X2"
                     /calculated_mol_wt=87016
     Region          7..87
                     /region_name="PDZ_ZASP52-like"
                     /note="PDZ domain of Drosophila melanogaster PDZ and LIM
                     domain protein Zasp52 (also known as Zasp), and related
                     domains; cd23068"
                     /db_xref="CDD:467281"
     Site            order(17..23,35,38,67,71,74..75)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467281"
     Region          150..>213
                     /region_name="DUF4749"
                     /note="Domain of unknown function (DUF4749); pfam15936"
                     /db_xref="CDD:464948"
     Region          272..323
                     /region_name="LIM_ALP_like"
                     /note="The LIM domain of ALP (actinin-associated LIM
                     protein) family; cd09360"
                     /db_xref="CDD:188746"
     Site            order(272,275,292,295,298,301,319,322)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188746"
     Region          <374..649
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          664..717
                     /region_name="LIM1_Enigma_like_1"
                     /note="The first LIM domain of an Enigma subfamily with
                     unknown function; cd09455"
                     /db_xref="CDD:188839"
     Site            order(664,667,684,687,690,693,713,716)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188839"
     Region          725..776
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(725,728,745,748,751,754,772,775)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:259829"
     Region          784..837
                     /region_name="LIM3_Enigma_like_1"
                     /note="The third LIM domain of an Enigma subfamily with
                     unknown function; cd09461"
                     /db_xref="CDD:188845"
     Site            order(784,787,806,809,812,815,833,836)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188845"
     CDS             1..838
                     /gene="Zasp52"
                     /coded_by="XM_077648734.1:742..3258"
                     /db_xref="GeneID:144114785"
ORIGIN      
        1 mnesvitvkl qrgdgtswgf rlaggkdfgy plsiqrvnpg slaekgglqt gdalisiqgk
       61 stdqlrhkea qdaimragny lelqvtrgas mtwkpkvtpv gsmpkpgpms nggpapvtkt
      121 slaankqpyk pigtghntaa kpfggtmqav vhnqynspmp vysmnniadt lsaqtevlts
      181 gavginfmkg dkplnkdsav yrmvleedkh pkdygsggss spalggpppa avtassptpa
      241 pmvnlrhvea pktapapppg qpnttvgapn tcadcgrliv gvfvrikdra lhaecfrcat
      301 cgtslknigy fnikdklycd ihakqaarhi apgsnleavp vppgghipqg vplapapapa
      361 pavvpvapra pagavspqgp lytppapkyt paaapytptq aapvhspvaa apladqpppp
      421 pdspgicyds pvamgrsdfr qapthspvag skpltpqsak ttppfggvtp lhpvtapvgd
      481 ahrpvpggak ftwppaqagg ptplssgvle snatpsvpty rpppgtqrva apstpkwggp
      541 sqapggpfrp sspsaspysp vsspassrpp sfvssspspl sgfggqpgvk vppptapkpv
      601 arapsakmgn ggpwgspapa lstgassapf tgpsglsqga gsrpapkrgr gkltqaiggk
      661 ipicsscgsp irgpfvtalg rnwcpehflc sngscrkslq digfveeqgk lycehcyecy
      721 mapicrkcgh rikgdclnal eqtwhpecfv ctycktafgn ssfyledgmp ycekdwnelf
      781 ttkcvgcgfp ieagdrwvea lnnnyhsqcf kcticqknle gqsffakggr pfckahar
//
LOCUS       XP_077504861             759 aa            linear   INV 25-NOV-2025
DEFINITION  Z band alternatively spliced PDZ-motif protein 52 isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077504861
VERSION     XP_077504861.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648735.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..759
                     /product="Z band alternatively spliced PDZ-motif protein
                     52 isoform X3"
                     /calculated_mol_wt=80174
     Region          7..87
                     /region_name="PDZ_ZASP52-like"
                     /note="PDZ domain of Drosophila melanogaster PDZ and LIM
                     domain protein Zasp52 (also known as Zasp), and related
                     domains; cd23068"
                     /db_xref="CDD:467281"
     Site            order(17..23,35,38,67,71,74..75)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467281"
     Region          150..>212
                     /region_name="DUF4749"
                     /note="Domain of unknown function (DUF4749); pfam15936"
                     /db_xref="CDD:464948"
     Region          312..363
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(312,315,332,335,338,341,359,362)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:259829"
     Region          <371..536
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          585..638
                     /region_name="LIM1_Enigma_like_1"
                     /note="The first LIM domain of an Enigma subfamily with
                     unknown function; cd09455"
                     /db_xref="CDD:188839"
     Site            order(585,588,605,608,611,614,634,637)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188839"
     Region          646..697
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(646,649,666,669,672,675,693,696)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:259829"
     Region          705..758
                     /region_name="LIM3_Enigma_like_1"
                     /note="The third LIM domain of an Enigma subfamily with
                     unknown function; cd09461"
                     /db_xref="CDD:188845"
     Site            order(705,708,727,730,733,736,754,757)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188845"
     CDS             1..759
                     /gene="Zasp52"
                     /coded_by="XM_077648735.1:567..2846"
                     /db_xref="GeneID:144114785"
ORIGIN      
        1 mnesvitvkl qrgdgtswgf rlaggkdfgy plsiqrvnpg slaekgglqt gdalisiqgk
       61 stdqlrhkea qdaimragny lelqvtrgas mtwkpkvtpv gsmpkpgpms nggpapvtkt
      121 slaankqpyk pigtghntaa kpfggtmqav vhnqynspmp vysmnniadt lsaqtevlts
      181 gavginfmkg dkplnkdsav yrmvleedkh pkgdesdlvs vaetrtqsla frkllaalgd
      241 dddclrsshd vtdygsggss spalggpppa avtassptpa pmvnlrhvea pktapapppg
      301 qpnttvgapn tcadcgrliv gvfvrikdra lhaecfrcat cgtslknigy fnikdklycd
      361 ihakqaarhi apgsnleavp vppgghipqg vplapapapa pavvpvapra pagavspqgp
      421 lytppapkyt paaapytptq aapvhspvaa apladqpppp pdspgicyds pvamgrsdfr
      481 qapthspvag skpltpqsak ttppfggvtp lhpvtapvgd ahrpvpante sttpytngig
      541 nghaheepel edileclsqg agsrpapkrg rgkltqaigg kipicsscgs pirgpfvtal
      601 grnwcpehfl csngscrksl qdigfveeqg klycehcyec ymapicrkcg hrikgdclna
      661 leqtwhpecf vctycktafg nssfyledgm pycekdwnel fttkcvgcgf pieagdrwve
      721 alnnnyhsqc fkcticqknl egqsffakgg rpfckahar
//
LOCUS       XP_077504862             730 aa            linear   INV 25-NOV-2025
DEFINITION  Z band alternatively spliced PDZ-motif protein 52 isoform X4
            [Amblyomma americanum].
ACCESSION   XP_077504862
VERSION     XP_077504862.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648736.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..730
                     /product="Z band alternatively spliced PDZ-motif protein
                     52 isoform X4"
                     /calculated_mol_wt=77006
     Region          7..87
                     /region_name="PDZ_ZASP52-like"
                     /note="PDZ domain of Drosophila melanogaster PDZ and LIM
                     domain protein Zasp52 (also known as Zasp), and related
                     domains; cd23068"
                     /db_xref="CDD:467281"
     Site            order(17..23,35,38,67,71,74..75)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467281"
     Region          150..>212
                     /region_name="DUF4749"
                     /note="Domain of unknown function (DUF4749); pfam15936"
                     /db_xref="CDD:464948"
     Region          312..363
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(312,315,332,335,338,341,359,362)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:259829"
     Region          <371..540
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          556..609
                     /region_name="LIM1_Enigma_like_1"
                     /note="The first LIM domain of an Enigma subfamily with
                     unknown function; cd09455"
                     /db_xref="CDD:188839"
     Site            order(556,559,576,579,582,585,605,608)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188839"
     Region          617..668
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(617,620,637,640,643,646,664,667)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:259829"
     Region          676..729
                     /region_name="LIM3_Enigma_like_1"
                     /note="The third LIM domain of an Enigma subfamily with
                     unknown function; cd09461"
                     /db_xref="CDD:188845"
     Site            order(676,679,698,701,704,707,725,728)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188845"
     CDS             1..730
                     /gene="Zasp52"
                     /coded_by="XM_077648736.1:567..2759"
                     /db_xref="GeneID:144114785"
ORIGIN      
        1 mnesvitvkl qrgdgtswgf rlaggkdfgy plsiqrvnpg slaekgglqt gdalisiqgk
       61 stdqlrhkea qdaimragny lelqvtrgas mtwkpkvtpv gsmpkpgpms nggpapvtkt
      121 slaankqpyk pigtghntaa kpfggtmqav vhnqynspmp vysmnniadt lsaqtevlts
      181 gavginfmkg dkplnkdsav yrmvleedkh pkgdesdlvs vaetrtqsla frkllaalgd
      241 dddclrsshd vtdygsggss spalggpppa avtassptpa pmvnlrhvea pktapapppg
      301 qpnttvgapn tcadcgrliv gvfvrikdra lhaecfrcat cgtslknigy fnikdklycd
      361 ihakqaarhi apgsnleavp vppgghipqg vplapapapa pavvpvapra pagavspqgp
      421 lytppapkyt paaapytptq aapvhspvaa apladqpppp pdspgicyds pvamgrsdfr
      481 qapthspvag skpltpqsak ttppfggvtp lhpvtapvgd ahrpvpglsq gagsrpapkr
      541 grgkltqaig gkipicsscg spirgpfvta lgrnwcpehf lcsngscrks lqdigfveeq
      601 gklycehcye cymapicrkc ghrikgdcln aleqtwhpec fvctycktaf gnssfyledg
      661 mpycekdwne lfttkcvgcg fpieagdrwv ealnnnyhsq cfkcticqkn legqsffakg
      721 grpfckahar
//
LOCUS       XP_077504863             719 aa            linear   INV 25-NOV-2025
DEFINITION  Z band alternatively spliced PDZ-motif protein 52 isoform X5
            [Amblyomma americanum].
ACCESSION   XP_077504863
VERSION     XP_077504863.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648737.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..719
                     /product="Z band alternatively spliced PDZ-motif protein
                     52 isoform X5"
                     /calculated_mol_wt=75884
     Region          7..87
                     /region_name="PDZ_ZASP52-like"
                     /note="PDZ domain of Drosophila melanogaster PDZ and LIM
                     domain protein Zasp52 (also known as Zasp), and related
                     domains; cd23068"
                     /db_xref="CDD:467281"
     Site            order(17..23,35,38,67,71,74..75)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467281"
     Region          150..>213
                     /region_name="DUF4749"
                     /note="Domain of unknown function (DUF4749); pfam15936"
                     /db_xref="CDD:464948"
     Region          272..323
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(272,275,292,295,298,301,319,322)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:259829"
     Region          <331..496
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          545..598
                     /region_name="LIM1_Enigma_like_1"
                     /note="The first LIM domain of an Enigma subfamily with
                     unknown function; cd09455"
                     /db_xref="CDD:188839"
     Site            order(545,548,565,568,571,574,594,597)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188839"
     Region          606..657
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(606,609,626,629,632,635,653,656)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:259829"
     Region          665..718
                     /region_name="LIM3_Enigma_like_1"
                     /note="The third LIM domain of an Enigma subfamily with
                     unknown function; cd09461"
                     /db_xref="CDD:188845"
     Site            order(665,668,687,690,693,696,714,717)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188845"
     CDS             1..719
                     /gene="Zasp52"
                     /coded_by="XM_077648737.1:567..2726"
                     /db_xref="GeneID:144114785"
ORIGIN      
        1 mnesvitvkl qrgdgtswgf rlaggkdfgy plsiqrvnpg slaekgglqt gdalisiqgk
       61 stdqlrhkea qdaimragny lelqvtrgas mtwkpkvtpv gsmpkpgpms nggpapvtkt
      121 slaankqpyk pigtghntaa kpfggtmqav vhnqynspmp vysmnniadt lsaqtevlts
      181 gavginfmkg dkplnkdsav yrmvleedkh pkdygsggss spalggpppa avtassptpa
      241 pmvnlrhvea pktapapppg qpnttvgapn tcadcgrliv gvfvrikdra lhaecfrcat
      301 cgtslknigy fnikdklycd ihakqaarhi apgsnleavp vppgghipqg vplapapapa
      361 pavvpvapra pagavspqgp lytppapkyt paaapytptq aapvhspvaa apladqpppp
      421 pdspgicyds pvamgrsdfr qapthspvag skpltpqsak ttppfggvtp lhpvtapvgd
      481 ahrpvpante sttpytngig nghaheepel edileclsqg agsrpapkrg rgkltqaigg
      541 kipicsscgs pirgpfvtal grnwcpehfl csngscrksl qdigfveeqg klycehcyec
      601 ymapicrkcg hrikgdclna leqtwhpecf vctycktafg nssfyledgm pycekdwnel
      661 fttkcvgcgf pieagdrwve alnnnyhsqc fkcticqknl egqsffakgg rpfckahar
//
LOCUS       XP_077504864             690 aa            linear   INV 25-NOV-2025
DEFINITION  Z band alternatively spliced PDZ-motif protein 52 isoform X6
            [Amblyomma americanum].
ACCESSION   XP_077504864
VERSION     XP_077504864.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648738.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..690
                     /product="Z band alternatively spliced PDZ-motif protein
                     52 isoform X6"
                     /calculated_mol_wt=72717
     Region          7..87
                     /region_name="PDZ_ZASP52-like"
                     /note="PDZ domain of Drosophila melanogaster PDZ and LIM
                     domain protein Zasp52 (also known as Zasp), and related
                     domains; cd23068"
                     /db_xref="CDD:467281"
     Site            order(17..23,35,38,67,71,74..75)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467281"
     Region          150..>213
                     /region_name="DUF4749"
                     /note="Domain of unknown function (DUF4749); pfam15936"
                     /db_xref="CDD:464948"
     Region          272..323
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(272,275,292,295,298,301,319,322)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:259829"
     Region          <331..500
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          516..569
                     /region_name="LIM1_Enigma_like_1"
                     /note="The first LIM domain of an Enigma subfamily with
                     unknown function; cd09455"
                     /db_xref="CDD:188839"
     Site            order(516,519,536,539,542,545,565,568)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188839"
     Region          577..628
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(577,580,597,600,603,606,624,627)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:259829"
     Region          636..689
                     /region_name="LIM3_Enigma_like_1"
                     /note="The third LIM domain of an Enigma subfamily with
                     unknown function; cd09461"
                     /db_xref="CDD:188845"
     Site            order(636,639,658,661,664,667,685,688)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188845"
     CDS             1..690
                     /gene="Zasp52"
                     /coded_by="XM_077648738.1:567..2639"
                     /db_xref="GeneID:144114785"
ORIGIN      
        1 mnesvitvkl qrgdgtswgf rlaggkdfgy plsiqrvnpg slaekgglqt gdalisiqgk
       61 stdqlrhkea qdaimragny lelqvtrgas mtwkpkvtpv gsmpkpgpms nggpapvtkt
      121 slaankqpyk pigtghntaa kpfggtmqav vhnqynspmp vysmnniadt lsaqtevlts
      181 gavginfmkg dkplnkdsav yrmvleedkh pkdygsggss spalggpppa avtassptpa
      241 pmvnlrhvea pktapapppg qpnttvgapn tcadcgrliv gvfvrikdra lhaecfrcat
      301 cgtslknigy fnikdklycd ihakqaarhi apgsnleavp vppgghipqg vplapapapa
      361 pavvpvapra pagavspqgp lytppapkyt paaapytptq aapvhspvaa apladqpppp
      421 pdspgicyds pvamgrsdfr qapthspvag skpltpqsak ttppfggvtp lhpvtapvgd
      481 ahrpvpglsq gagsrpapkr grgkltqaig gkipicsscg spirgpfvta lgrnwcpehf
      541 lcsngscrks lqdigfveeq gklycehcye cymapicrkc ghrikgdcln aleqtwhpec
      601 fvctycktaf gnssfyledg mpycekdwne lfttkcvgcg fpieagdrwv ealnnnyhsq
      661 cfkcticqkn legqsffakg grpfckahar
//
LOCUS       XP_077504865             687 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114786 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504865
VERSION     XP_077504865.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648739.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..687
                     /product="uncharacterized protein LOC144114786 isoform X1"
                     /calculated_mol_wt=79332
     Region          9..89
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cl49608"
                     /db_xref="CDD:483948"
     Site            order(19..25,37,40,69,73,76..77)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467153"
     Region          106..133
                     /region_name="ZM"
                     /note="ZASP-like motif; smart00735"
                     /db_xref="CDD:128974"
     Region          107..>173
                     /region_name="DUF4749"
                     /note="Domain of unknown function (DUF4749); pfam15936"
                     /db_xref="CDD:464948"
     CDS             1..687
                     /gene="LOC144114786"
                     /coded_by="XM_077648739.1:430..2493"
                     /db_xref="GeneID:144114786"
ORIGIN      
        1 mafqrpflii lkredpgqpw gfrvsggadl atpprvdkvv dggpaalqgm hegdliiqvg
       61 khnvqdltea rinsllaqpa krlevfivre dflqkprkge pstvkavvhn qynsplqlys
      121 qekvaetlay haqaitaedg vndiqpvglp ktlvrsavlq altedetgan pyrtangttv
      181 vqratapptp ppkprppsys sgspqperpy sssghhvtfs npvtpgtppv qqhhtyippp
      241 pplhsyqqps yqqpsyqqps yqqpsyqqps yqqpsyqqps yqqpsyqqps yqqqpasypt
      301 qharqssyee adrrqqqire aelrqrqyee erlqqaqyee grfrqppqdf rraeydaeqr
      361 rlreeeerhq rvfreeekrr qqeeqerkrr qeqqeyeqyq qhqqqyqryl eeqkrrddel
      421 rrrnagqqqq qqqqqphhqy hqqqqqhqqq eaarrtqlee qqrraqqqqq qqryqnvqre
      481 plnvqraqee aqrryqpsta vasspqhqeq vgsqaqrwes riweeqqarm pgtplgspsv
      541 srrardnvwp pkessvrwtp ktgfspqlar qssqgakqfv wpppktgvan gdegfpsrpp
      601 srsavnawhp asptsgtrtp pfarspslgr rtrdiawppp qadaspkfvp rpvsrtglhr
      661 pdefihqnas cgvpptyrpp pnssqyl
//
LOCUS       XP_077504866             636 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114786 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504866
VERSION     XP_077504866.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648740.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..636
                     /product="uncharacterized protein LOC144114786 isoform X2"
                     /calculated_mol_wt=73649
     Region          9..89
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cl49608"
                     /db_xref="CDD:483948"
     Site            order(19..25,37,40,69,73,76..77)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467153"
     CDS             1..636
                     /gene="LOC144114786"
                     /coded_by="XM_077648740.1:328..2238"
                     /db_xref="GeneID:144114786"
ORIGIN      
        1 mafqrpflii lkredpgqpw gfrvsggadl atpprvdkvv dggpaalqgm hegdliiqvg
       61 khnvqdltea rinsllaqpa krlevfivrv ndiqpvglpk tlvrsavlqa ltedetganp
      121 yrtangttvv qratapptpp pkprppsyss gspqperpys ssghhvtfsn pvtpgtppvq
      181 qhhtyipppp plhsyqqpsy qqpsyqqpsy qqpsyqqpsy qqpsyqqpsy qqpsyqqpsy
      241 qqqpasyptq harqssyeea drrqqqirea elrqrqyeee rlqqaqyeeg rfrqppqdfr
      301 raeydaeqrr lreeeerhqr vfreeekrrq qeeqerkrrq eqqeyeqyqq hqqqyqryle
      361 eqkrrddelr rrnagqqqqq qqqqphhqyh qqqqqhqqqe aarrtqleeq qrraqqqqqq
      421 qryqnvqrep lnvqraqeea qrryqpstav asspqhqeqv gsqaqrwesr iweeqqarmp
      481 gtplgspsvs rrardnvwpp kessvrwtpk tgfspqlarq ssqgakqfvw pppktgvang
      541 degfpsrpps rsavnawhpa sptsgtrtpp farspslgrr trdiawpppq adaspkfvpr
      601 pvsrtglhrp defihqnasc gvpptyrppp nssqyl
//
LOCUS       XP_077504867             532 aa            linear   INV 25-NOV-2025
DEFINITION  serine/threonine receptor kinase thickveins [Amblyomma americanum].
ACCESSION   XP_077504867
VERSION     XP_077504867.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648741.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (DAPXPI010005483.1) annotated
            using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 134 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..532
                     /product="serine/threonine receptor kinase thickveins"
                     /calculated_mol_wt=59385
     Region          39..126
                     /region_name="TFP_LU_ECD_Tkv"
                     /note="extracellular domain (ECD) found in Drosophila
                     melanogaster receptor protein serine/threonine kinase
                     Thickveins and similar proteins; cd23596"
                     /db_xref="CDD:467125"
     Region          202..228
                     /region_name="TGF_beta_GS"
                     /note="Transforming growth factor beta type I GS-motif;
                     pfam08515"
                     /db_xref="CDD:462503"
     Region          235..520
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(236..239,242,244,255,257,285,305..308,358,362..363,
                     365,375..376)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..532
                     /gene="tkv"
                     /coded_by="XM_077648741.1:8..1606"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 134 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144114787"
ORIGIN      
        1 matggsrhcc lkmrtrlkam lwllpcvsla llvaegnale cyclgscpdg sfngtctapp
       61 dsdcfsavee vlnpetgqle pertygclps eegsimwcka hlvphklpks iaccresdlc
      121 nkylkpmyrk rtddeygptv ylykdpylqi vifvvvlgva aclvvigyif ysrhkkales
      181 rsffasgche pflpideggp clkdliehsi tsgsgsglpq lvqrtiarqi qmiesigkgr
      241 ygevwkahwr geyvavkvff ttdeaswlre tdiyqtmllr hdnilgfvas dirgtgswtq
      301 mllitnfher gslhdylsth aldpddavrl ahsaacglsh lhieiigkqg kpaiahrdik
      361 sknilvkkdg tcaiadfgla vrfsseanel diavnprvgt krymapevld etlvpshfds
      421 yrmadmysfg lvlweitlrc ivdgaveeyr ipyhdvvpsd psfedmrkvv cvdnarpqlp
      481 krwsssqvtq clsklmqecw hqrpgvrlta lrvkkslarl lvennsiggk mv
//
LOCUS       XP_077504868             141 aa            linear   INV 25-NOV-2025
DEFINITION  histone H2A-like [Amblyomma americanum].
ACCESSION   XP_077504868
VERSION     XP_077504868.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648742.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="histone H2A-like"
                     /calculated_mol_wt=14728
     Region          5..133
                     /region_name="PTZ00017"
                     /note="histone H2A; Provisional"
                     /db_xref="CDD:185399"
     CDS             1..141
                     /gene="LOC144114788"
                     /coded_by="XM_077648742.1:370..795"
                     /db_xref="GeneID:144114788"
ORIGIN      
        1 masrgagakt skapkaksrs araglqfpvg rihrllrkgn yaervgagap vymaavleyl
       61 aaevlelagn aardnnktri tprhiqlavr ndeelsklls gvtiseggvl phipaellpk
      121 ksggpshngr kgepgaasqd y
//
LOCUS       XP_077504869             514 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial calcium uptake 1 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504869
VERSION     XP_077504869.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648743.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..514
                     /product="mitochondrial calcium uptake 1 isoform X1"
                     /calculated_mol_wt=59197
     Region          261..481
                     /region_name="EFh_MICU"
                     /note="EF-hand, calcium binding motif, found in
                     mitochondrial calcium uptake proteins MICU1, MICU2, MICU3,
                     and similar proteins; cd15900"
                     /db_xref="CDD:320080"
     Region          261..290
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320080"
     Site            order(270,272,274,281,461,463,465,472)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320080"
     Region          315..414
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320080"
     Region          415..444
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320080"
     Region          452..481
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320080"
     CDS             1..514
                     /gene="MICU1"
                     /coded_by="XM_077648743.1:200..1744"
                     /db_xref="GeneID:144114789"
ORIGIN      
        1 mwpllrrslq iavrlrcchs sryvfgaqri lavntsvers slrthynfgh kkrdtpshek
       61 alfavlilms fglfidfdwv adktgdgiea lkkyfrvsse ssvshpsese dedadveeak
      121 gkgkgkkkkd kpgfrdrkii eyenrirays tpdkifryfa tmrvyneagd seifmspqdf
      181 lrsitpgtkq pegfgldqyk ridirtdnas gngykvsadm dekvfsglse dsifyklsss
      241 glisfsdyif lltilsaskr qfeiafrmfd lngdgnvdyd efekvqtiir hqtsfgmrhr
      301 dhqntgsiyr gvssalcnyf fgpkldkklt vekflnfqrq lqteiltlef mrkepdehgm
      361 ikewdfadll ltyaglndkk ksrmikrikk kfknqsegis ledylqffhf lsnitevdta
      421 ltfyhiagas ideatlkhva rtvahvdlrd yvidviftlf denmdgqlsn kefvsvmker
      481 lhrglekpkd tgfvklinsa wkcaklkkpv lldi
//
LOCUS       XP_077504870             499 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial calcium uptake 1 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504870
VERSION     XP_077504870.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648744.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..499
                     /product="mitochondrial calcium uptake 1 isoform X2"
                     /calculated_mol_wt=57685
     Region          246..466
                     /region_name="EFh_MICU"
                     /note="EF-hand, calcium binding motif, found in
                     mitochondrial calcium uptake proteins MICU1, MICU2, MICU3,
                     and similar proteins; cd15900"
                     /db_xref="CDD:320080"
     Region          246..275
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320080"
     Site            order(255,257,259,266,446,448,450,457)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320080"
     Region          300..399
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320080"
     Region          400..429
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320080"
     Region          437..466
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320080"
     CDS             1..499
                     /gene="MICU1"
                     /coded_by="XM_077648744.1:208..1707"
                     /db_xref="GeneID:144114789"
ORIGIN      
        1 mwpllrrslq iavrlrcchs sryvfgaqri lavntsvers slrthynfgh kkrdtpshek
       61 alfavlilms fglfidfdwv adktgdgiea lkkyfrvsse ssvshpsese dedadveeak
      121 gkgkgkkkkd kpgfrdrkii eyenrirays tpdkifryfa tmrvyneagd seifmspqdf
      181 lrsitpgtkq pegfgldqyk ridirdekvf sglsedsify klsssglisf sdyiflltil
      241 saskrqfeia frmfdlngdg nvdydefekv qtiirhqtsf gmrhrdhqnt gsiyrgvssa
      301 lcnyffgpkl dkkltvekfl nfqrqlqtei ltlefmrkep dehgmikewd fadllltyag
      361 lndkkksrmi krikkkfknq segisledyl qffhflsnit evdtaltfyh iagasideat
      421 lkhvartvah vdlrdyvidv iftlfdenmd gqlsnkefvs vmkerlhrgl ekpkdtgfvk
      481 linsawkcak lkkpvlldi
//
LOCUS       XP_077504871             426 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial calcium uptake 1 isoform X3 [Amblyomma americanum].
ACCESSION   XP_077504871
VERSION     XP_077504871.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648745.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..426
                     /product="mitochondrial calcium uptake 1 isoform X3"
                     /calculated_mol_wt=48895
     Region          <205..283
                     /region_name="PPP2R3"
                     /note="serine/threonine protein phosphatase 2A regulatory
                     subunit B'; cl41751"
                     /db_xref="CDD:425382"
     Region          261..>345
                     /region_name="EFh_MICU"
                     /note="EF-hand, calcium binding motif, found in
                     mitochondrial calcium uptake proteins MICU1, MICU2, MICU3,
                     and similar proteins; cd15900"
                     /db_xref="CDD:320080"
     Region          261..290
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320080"
     Region          315..345
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320080"
     CDS             1..426
                     /gene="MICU1"
                     /coded_by="XM_077648745.1:196..1476"
                     /db_xref="GeneID:144114789"
ORIGIN      
        1 mwpllrrslq iavrlrcchs sryvfgaqri lavntsvers slrthynfgh kkrdtpshek
       61 alfavlilms fglfidfdwv adktgdgiea lkkyfrvsse ssvshpsese dedadveeak
      121 gkgkgkkkkd kpgfrdrkii eyenrirays tpdkifryfa tmrvyneagd seifmspqdf
      181 lrsitpgtkq pegfgldqyk ridirtdnas gngykvsadm dekvfsglse dsifyklsss
      241 glisfsdyif lltilsaskr qfeiafrmfd lngdgnvdyd efekvqtiir hqtsfgmrhr
      301 dhqntgsiyr gvssalcnyf fgpkldkklt vekflnfqrq lqteiltlef mrkepdehgm
      361 ikewdfadll ltyaglndkk ksrmikrikk kfknqsecsq safekiffis ealyptgpgi
      421 cggckv
//
LOCUS       XP_077504872            1989 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114790 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504872
VERSION     XP_077504872.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648746.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1989
                     /product="uncharacterized protein LOC144114790 isoform X1"
                     /calculated_mol_wt=220483
     Region          128..178
                     /region_name="H15"
                     /note="Domain in histone families 1 and 5; smart00526"
                     /db_xref="CDD:197772"
     Site            159..170
                     /site_type="DNA binding"
                     /note="DNA-binding site [nucleotide binding]"
                     /db_xref="CDD:238028"
     Region          228..281
                     /region_name="PHD_SF"
                     /note="PHD finger superfamily; cl22851"
                     /db_xref="CDD:473978"
     Site            order(246..249,253,276)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:276966"
     Region          283..328
                     /region_name="PHD_SF"
                     /note="PHD finger superfamily; cl22851"
                     /db_xref="CDD:473978"
     Site            order(283,296..299,303,323)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:276966"
     Region          <466..743
                     /region_name="PLN00104"
                     /note="MYST -like histone acetyltransferase; Provisional"
                     /db_xref="CDD:215056"
     Region          <798..1191
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          <1507..1688
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..1989
                     /gene="LOC144114790"
                     /coded_by="XM_077648746.1:254..6223"
                     /db_xref="GeneID:144114790"
ORIGIN      
        1 mreqqdvagl slgqarrlve airkiksqkq rpslerishv vglradlvdr qleqaitrgf
       61 ilkvmnkgqc sykdaetlkl avkkkcaats lvsstaaggg raraaavaaa aaigagihrr
      121 kdlmhsavea vralgekggs tfpaivkfvr qalrmdpgir lrlaikravs aghllrvgrl
      181 yqlpcdesrs esdsddevgt sdrlgagkng dlagrtghkr rsalqgrcsv chgmprnnks
      241 gvaeellsch sctfsahpsc lkhmkelavv rlnskwqcpq crtcsrcgtr keeehllcce
      301 vcdshfhlgc lkppllkapk gswkcascss rrlksinfvn nlaskikqrn kryhngmmrk
      361 qriptlerpt tdgvkvfrsk lhkrkdikqv aslratqhan cfapsrsssk keipkstmrs
      421 trdtpsdlpp svsekdlkif kktqeralke mgqdlvvpdh qtrcpaaief gqyeiqtwys
      481 spypqeyarl pklflcefcl kymksrnils rhmrkctwfh ppateiyrkg evsvfevdgn
      541 vskiycqnlc llaklfldhk tlyydvepfl fyvltkndtk gchlvgyfsk ekhcqqrynv
      601 scimtmpqyq rqgfgrflid fsyllsrkeg ltgtpekpls dlgrisymsy wksillefln
      661 nyketnisiq clssstglni hdiattlqhl nmfkkmgsek sriviridkq mlsehmakvh
      721 sgkdrrivld peclrwtplv tqpqfrdeep eeeeqqqqqq qqqqqqqqqq qqqqqekqqq
      781 qrhrqretpv veevkekkrp klmslaasva aeqrtrkqpa pvkrphrrrk rkipwlnrkp
      841 arsaprvpqa vsngkeesgp etaavvqevp rpparklrrl setakatepd aareepparr
      901 lrtkepaqap tataqvprsa sesvyphlks gpghkgmvlp pkksrrgvrl igmepqeqqe
      961 apivdpvvka vtnlgaqeve qaatasssat qqqpvgattr rrssrvesqp qlrprrtrsv
     1021 evskeecapp vlapedaplp avraskspaa addapkppvl eafengstgp atrrgtkrlk
     1081 rvqrrgrppk kqyipvespk pdevpntens dtesgkleep aeerhlpvtt eegsaspipq
     1141 qvssrpsspg dmavkeqite ppaivvstet nseenevsid tkeqppdqds sahaevvshs
     1201 vqseedvyke estvvrtakq dsgnsaalte mdsaeaesaa spvettseep rdsmvvegtq
     1261 hipdsaltsv petqseeeke sessvpkqqq esviegchfe qspresgshq dvpsvpecvd
     1321 ndlvdslepr pqitllpsda iannasvvst ssqesqdivv dnpspqvqmp ptpitpqtpg
     1381 snppsqqtcl snqsscgvee saptgsydsm lghspasmmg csphdlsmda sslkaappve
     1441 phryqeprev trgprgepme tarmemmrpg rlaapskpdl sclgvytpds ssssvmssgg
     1501 ytpvdmeqlr eagqlrevnq lremnslret nqlrdvsqlr dvnhlreanq lrdmnqlrdv
     1561 sqlrdvnhlr dinrlhdinq lremnqmrem nqmremnqmr emnqmreanq mrevsqmrev
     1621 sqmrevsqmr evsqmrevnq lrdinqlrdv sqlreasqlr disqirevnr hevnqlrdmn
     1681 qlrdplress plcdvnqlre mgqrrldtpa aslvageaqg alehlsspfa dcthsrppqa
     1741 hhrqqpqphr qtpqqsycrp vtsaplsqga fvaaqaavaq aqaqasagvp gvmqsmvlmp
     1801 pasnymsisg pgpapsyvmg vpvggmmaas gppqrgfshg yaygpgsnas cslaklqqlt
     1861 ngimdiapgs pgqsaaaaaa aaaacamtpp tapaasrals qsygsptaap pagsakyapr
     1921 yqrgpllsgy qgalngyrqg tptgpylqqp apmqmvqpqy qepsqnpmyt ygylsgslpp
     1981 qtlnsmmrr
//
LOCUS       XP_077504873            1989 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114790 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504873
VERSION     XP_077504873.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648747.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1989
                     /product="uncharacterized protein LOC144114790 isoform X1"
                     /calculated_mol_wt=220483
     Region          128..178
                     /region_name="H15"
                     /note="Domain in histone families 1 and 5; smart00526"
                     /db_xref="CDD:197772"
     Site            159..170
                     /site_type="DNA binding"
                     /note="DNA-binding site [nucleotide binding]"
                     /db_xref="CDD:238028"
     Region          228..281
                     /region_name="PHD_SF"
                     /note="PHD finger superfamily; cl22851"
                     /db_xref="CDD:473978"
     Site            order(246..249,253,276)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:276966"
     Region          283..328
                     /region_name="PHD_SF"
                     /note="PHD finger superfamily; cl22851"
                     /db_xref="CDD:473978"
     Site            order(283,296..299,303,323)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:276966"
     Region          <466..743
                     /region_name="PLN00104"
                     /note="MYST -like histone acetyltransferase; Provisional"
                     /db_xref="CDD:215056"
     Region          <798..1191
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          <1507..1688
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..1989
                     /gene="LOC144114790"
                     /coded_by="XM_077648747.1:266..6235"
                     /db_xref="GeneID:144114790"
ORIGIN      
        1 mreqqdvagl slgqarrlve airkiksqkq rpslerishv vglradlvdr qleqaitrgf
       61 ilkvmnkgqc sykdaetlkl avkkkcaats lvsstaaggg raraaavaaa aaigagihrr
      121 kdlmhsavea vralgekggs tfpaivkfvr qalrmdpgir lrlaikravs aghllrvgrl
      181 yqlpcdesrs esdsddevgt sdrlgagkng dlagrtghkr rsalqgrcsv chgmprnnks
      241 gvaeellsch sctfsahpsc lkhmkelavv rlnskwqcpq crtcsrcgtr keeehllcce
      301 vcdshfhlgc lkppllkapk gswkcascss rrlksinfvn nlaskikqrn kryhngmmrk
      361 qriptlerpt tdgvkvfrsk lhkrkdikqv aslratqhan cfapsrsssk keipkstmrs
      421 trdtpsdlpp svsekdlkif kktqeralke mgqdlvvpdh qtrcpaaief gqyeiqtwys
      481 spypqeyarl pklflcefcl kymksrnils rhmrkctwfh ppateiyrkg evsvfevdgn
      541 vskiycqnlc llaklfldhk tlyydvepfl fyvltkndtk gchlvgyfsk ekhcqqrynv
      601 scimtmpqyq rqgfgrflid fsyllsrkeg ltgtpekpls dlgrisymsy wksillefln
      661 nyketnisiq clssstglni hdiattlqhl nmfkkmgsek sriviridkq mlsehmakvh
      721 sgkdrrivld peclrwtplv tqpqfrdeep eeeeqqqqqq qqqqqqqqqq qqqqqekqqq
      781 qrhrqretpv veevkekkrp klmslaasva aeqrtrkqpa pvkrphrrrk rkipwlnrkp
      841 arsaprvpqa vsngkeesgp etaavvqevp rpparklrrl setakatepd aareepparr
      901 lrtkepaqap tataqvprsa sesvyphlks gpghkgmvlp pkksrrgvrl igmepqeqqe
      961 apivdpvvka vtnlgaqeve qaatasssat qqqpvgattr rrssrvesqp qlrprrtrsv
     1021 evskeecapp vlapedaplp avraskspaa addapkppvl eafengstgp atrrgtkrlk
     1081 rvqrrgrppk kqyipvespk pdevpntens dtesgkleep aeerhlpvtt eegsaspipq
     1141 qvssrpsspg dmavkeqite ppaivvstet nseenevsid tkeqppdqds sahaevvshs
     1201 vqseedvyke estvvrtakq dsgnsaalte mdsaeaesaa spvettseep rdsmvvegtq
     1261 hipdsaltsv petqseeeke sessvpkqqq esviegchfe qspresgshq dvpsvpecvd
     1321 ndlvdslepr pqitllpsda iannasvvst ssqesqdivv dnpspqvqmp ptpitpqtpg
     1381 snppsqqtcl snqsscgvee saptgsydsm lghspasmmg csphdlsmda sslkaappve
     1441 phryqeprev trgprgepme tarmemmrpg rlaapskpdl sclgvytpds ssssvmssgg
     1501 ytpvdmeqlr eagqlrevnq lremnslret nqlrdvsqlr dvnhlreanq lrdmnqlrdv
     1561 sqlrdvnhlr dinrlhdinq lremnqmrem nqmremnqmr emnqmreanq mrevsqmrev
     1621 sqmrevsqmr evsqmrevnq lrdinqlrdv sqlreasqlr disqirevnr hevnqlrdmn
     1681 qlrdplress plcdvnqlre mgqrrldtpa aslvageaqg alehlsspfa dcthsrppqa
     1741 hhrqqpqphr qtpqqsycrp vtsaplsqga fvaaqaavaq aqaqasagvp gvmqsmvlmp
     1801 pasnymsisg pgpapsyvmg vpvggmmaas gppqrgfshg yaygpgsnas cslaklqqlt
     1861 ngimdiapgs pgqsaaaaaa aaaacamtpp tapaasrals qsygsptaap pagsakyapr
     1921 yqrgpllsgy qgalngyrqg tptgpylqqp apmqmvqpqy qepsqnpmyt ygylsgslpp
     1981 qtlnsmmrr
//
LOCUS       XP_077504874            1989 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114790 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504874
VERSION     XP_077504874.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648748.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1989
                     /product="uncharacterized protein LOC144114790 isoform X1"
                     /calculated_mol_wt=220483
     Region          128..178
                     /region_name="H15"
                     /note="Domain in histone families 1 and 5; smart00526"
                     /db_xref="CDD:197772"
     Site            159..170
                     /site_type="DNA binding"
                     /note="DNA-binding site [nucleotide binding]"
                     /db_xref="CDD:238028"
     Region          228..281
                     /region_name="PHD_SF"
                     /note="PHD finger superfamily; cl22851"
                     /db_xref="CDD:473978"
     Site            order(246..249,253,276)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:276966"
     Region          283..328
                     /region_name="PHD_SF"
                     /note="PHD finger superfamily; cl22851"
                     /db_xref="CDD:473978"
     Site            order(283,296..299,303,323)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:276966"
     Region          <466..743
                     /region_name="PLN00104"
                     /note="MYST -like histone acetyltransferase; Provisional"
                     /db_xref="CDD:215056"
     Region          <798..1191
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          <1507..1688
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..1989
                     /gene="LOC144114790"
                     /coded_by="XM_077648748.1:167..6136"
                     /db_xref="GeneID:144114790"
ORIGIN      
        1 mreqqdvagl slgqarrlve airkiksqkq rpslerishv vglradlvdr qleqaitrgf
       61 ilkvmnkgqc sykdaetlkl avkkkcaats lvsstaaggg raraaavaaa aaigagihrr
      121 kdlmhsavea vralgekggs tfpaivkfvr qalrmdpgir lrlaikravs aghllrvgrl
      181 yqlpcdesrs esdsddevgt sdrlgagkng dlagrtghkr rsalqgrcsv chgmprnnks
      241 gvaeellsch sctfsahpsc lkhmkelavv rlnskwqcpq crtcsrcgtr keeehllcce
      301 vcdshfhlgc lkppllkapk gswkcascss rrlksinfvn nlaskikqrn kryhngmmrk
      361 qriptlerpt tdgvkvfrsk lhkrkdikqv aslratqhan cfapsrsssk keipkstmrs
      421 trdtpsdlpp svsekdlkif kktqeralke mgqdlvvpdh qtrcpaaief gqyeiqtwys
      481 spypqeyarl pklflcefcl kymksrnils rhmrkctwfh ppateiyrkg evsvfevdgn
      541 vskiycqnlc llaklfldhk tlyydvepfl fyvltkndtk gchlvgyfsk ekhcqqrynv
      601 scimtmpqyq rqgfgrflid fsyllsrkeg ltgtpekpls dlgrisymsy wksillefln
      661 nyketnisiq clssstglni hdiattlqhl nmfkkmgsek sriviridkq mlsehmakvh
      721 sgkdrrivld peclrwtplv tqpqfrdeep eeeeqqqqqq qqqqqqqqqq qqqqqekqqq
      781 qrhrqretpv veevkekkrp klmslaasva aeqrtrkqpa pvkrphrrrk rkipwlnrkp
      841 arsaprvpqa vsngkeesgp etaavvqevp rpparklrrl setakatepd aareepparr
      901 lrtkepaqap tataqvprsa sesvyphlks gpghkgmvlp pkksrrgvrl igmepqeqqe
      961 apivdpvvka vtnlgaqeve qaatasssat qqqpvgattr rrssrvesqp qlrprrtrsv
     1021 evskeecapp vlapedaplp avraskspaa addapkppvl eafengstgp atrrgtkrlk
     1081 rvqrrgrppk kqyipvespk pdevpntens dtesgkleep aeerhlpvtt eegsaspipq
     1141 qvssrpsspg dmavkeqite ppaivvstet nseenevsid tkeqppdqds sahaevvshs
     1201 vqseedvyke estvvrtakq dsgnsaalte mdsaeaesaa spvettseep rdsmvvegtq
     1261 hipdsaltsv petqseeeke sessvpkqqq esviegchfe qspresgshq dvpsvpecvd
     1321 ndlvdslepr pqitllpsda iannasvvst ssqesqdivv dnpspqvqmp ptpitpqtpg
     1381 snppsqqtcl snqsscgvee saptgsydsm lghspasmmg csphdlsmda sslkaappve
     1441 phryqeprev trgprgepme tarmemmrpg rlaapskpdl sclgvytpds ssssvmssgg
     1501 ytpvdmeqlr eagqlrevnq lremnslret nqlrdvsqlr dvnhlreanq lrdmnqlrdv
     1561 sqlrdvnhlr dinrlhdinq lremnqmrem nqmremnqmr emnqmreanq mrevsqmrev
     1621 sqmrevsqmr evsqmrevnq lrdinqlrdv sqlreasqlr disqirevnr hevnqlrdmn
     1681 qlrdplress plcdvnqlre mgqrrldtpa aslvageaqg alehlsspfa dcthsrppqa
     1741 hhrqqpqphr qtpqqsycrp vtsaplsqga fvaaqaavaq aqaqasagvp gvmqsmvlmp
     1801 pasnymsisg pgpapsyvmg vpvggmmaas gppqrgfshg yaygpgsnas cslaklqqlt
     1861 ngimdiapgs pgqsaaaaaa aaaacamtpp tapaasrals qsygsptaap pagsakyapr
     1921 yqrgpllsgy qgalngyrqg tptgpylqqp apmqmvqpqy qepsqnpmyt ygylsgslpp
     1981 qtlnsmmrr
//
LOCUS       XP_077504875            1989 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114790 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504875
VERSION     XP_077504875.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648749.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1989
                     /product="uncharacterized protein LOC144114790 isoform X1"
                     /calculated_mol_wt=220483
     Region          128..178
                     /region_name="H15"
                     /note="Domain in histone families 1 and 5; smart00526"
                     /db_xref="CDD:197772"
     Site            159..170
                     /site_type="DNA binding"
                     /note="DNA-binding site [nucleotide binding]"
                     /db_xref="CDD:238028"
     Region          228..281
                     /region_name="PHD_SF"
                     /note="PHD finger superfamily; cl22851"
                     /db_xref="CDD:473978"
     Site            order(246..249,253,276)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:276966"
     Region          283..328
                     /region_name="PHD_SF"
                     /note="PHD finger superfamily; cl22851"
                     /db_xref="CDD:473978"
     Site            order(283,296..299,303,323)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:276966"
     Region          <466..743
                     /region_name="PLN00104"
                     /note="MYST -like histone acetyltransferase; Provisional"
                     /db_xref="CDD:215056"
     Region          <798..1191
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          <1507..1688
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..1989
                     /gene="LOC144114790"
                     /coded_by="XM_077648749.1:233..6202"
                     /db_xref="GeneID:144114790"
ORIGIN      
        1 mreqqdvagl slgqarrlve airkiksqkq rpslerishv vglradlvdr qleqaitrgf
       61 ilkvmnkgqc sykdaetlkl avkkkcaats lvsstaaggg raraaavaaa aaigagihrr
      121 kdlmhsavea vralgekggs tfpaivkfvr qalrmdpgir lrlaikravs aghllrvgrl
      181 yqlpcdesrs esdsddevgt sdrlgagkng dlagrtghkr rsalqgrcsv chgmprnnks
      241 gvaeellsch sctfsahpsc lkhmkelavv rlnskwqcpq crtcsrcgtr keeehllcce
      301 vcdshfhlgc lkppllkapk gswkcascss rrlksinfvn nlaskikqrn kryhngmmrk
      361 qriptlerpt tdgvkvfrsk lhkrkdikqv aslratqhan cfapsrsssk keipkstmrs
      421 trdtpsdlpp svsekdlkif kktqeralke mgqdlvvpdh qtrcpaaief gqyeiqtwys
      481 spypqeyarl pklflcefcl kymksrnils rhmrkctwfh ppateiyrkg evsvfevdgn
      541 vskiycqnlc llaklfldhk tlyydvepfl fyvltkndtk gchlvgyfsk ekhcqqrynv
      601 scimtmpqyq rqgfgrflid fsyllsrkeg ltgtpekpls dlgrisymsy wksillefln
      661 nyketnisiq clssstglni hdiattlqhl nmfkkmgsek sriviridkq mlsehmakvh
      721 sgkdrrivld peclrwtplv tqpqfrdeep eeeeqqqqqq qqqqqqqqqq qqqqqekqqq
      781 qrhrqretpv veevkekkrp klmslaasva aeqrtrkqpa pvkrphrrrk rkipwlnrkp
      841 arsaprvpqa vsngkeesgp etaavvqevp rpparklrrl setakatepd aareepparr
      901 lrtkepaqap tataqvprsa sesvyphlks gpghkgmvlp pkksrrgvrl igmepqeqqe
      961 apivdpvvka vtnlgaqeve qaatasssat qqqpvgattr rrssrvesqp qlrprrtrsv
     1021 evskeecapp vlapedaplp avraskspaa addapkppvl eafengstgp atrrgtkrlk
     1081 rvqrrgrppk kqyipvespk pdevpntens dtesgkleep aeerhlpvtt eegsaspipq
     1141 qvssrpsspg dmavkeqite ppaivvstet nseenevsid tkeqppdqds sahaevvshs
     1201 vqseedvyke estvvrtakq dsgnsaalte mdsaeaesaa spvettseep rdsmvvegtq
     1261 hipdsaltsv petqseeeke sessvpkqqq esviegchfe qspresgshq dvpsvpecvd
     1321 ndlvdslepr pqitllpsda iannasvvst ssqesqdivv dnpspqvqmp ptpitpqtpg
     1381 snppsqqtcl snqsscgvee saptgsydsm lghspasmmg csphdlsmda sslkaappve
     1441 phryqeprev trgprgepme tarmemmrpg rlaapskpdl sclgvytpds ssssvmssgg
     1501 ytpvdmeqlr eagqlrevnq lremnslret nqlrdvsqlr dvnhlreanq lrdmnqlrdv
     1561 sqlrdvnhlr dinrlhdinq lremnqmrem nqmremnqmr emnqmreanq mrevsqmrev
     1621 sqmrevsqmr evsqmrevnq lrdinqlrdv sqlreasqlr disqirevnr hevnqlrdmn
     1681 qlrdplress plcdvnqlre mgqrrldtpa aslvageaqg alehlsspfa dcthsrppqa
     1741 hhrqqpqphr qtpqqsycrp vtsaplsqga fvaaqaavaq aqaqasagvp gvmqsmvlmp
     1801 pasnymsisg pgpapsyvmg vpvggmmaas gppqrgfshg yaygpgsnas cslaklqqlt
     1861 ngimdiapgs pgqsaaaaaa aaaacamtpp tapaasrals qsygsptaap pagsakyapr
     1921 yqrgpllsgy qgalngyrqg tptgpylqqp apmqmvqpqy qepsqnpmyt ygylsgslpp
     1981 qtlnsmmrr
//
LOCUS       XP_077504876            1971 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114790 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504876
VERSION     XP_077504876.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648750.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1971
                     /product="uncharacterized protein LOC144114790 isoform X2"
                     /calculated_mol_wt=218346
     Region          128..178
                     /region_name="H15"
                     /note="Domain in histone families 1 and 5; smart00526"
                     /db_xref="CDD:197772"
     Site            159..170
                     /site_type="DNA binding"
                     /note="DNA-binding site [nucleotide binding]"
                     /db_xref="CDD:238028"
     Region          228..281
                     /region_name="PHD_SF"
                     /note="PHD finger superfamily; cl22851"
                     /db_xref="CDD:473978"
     Site            order(246..249,253,276)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:276966"
     Region          283..328
                     /region_name="PHD_SF"
                     /note="PHD finger superfamily; cl22851"
                     /db_xref="CDD:473978"
     Site            order(283,296..299,303,323)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:276966"
     Region          <448..725
                     /region_name="PLN00104"
                     /note="MYST -like histone acetyltransferase; Provisional"
                     /db_xref="CDD:215056"
     Region          <780..1173
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          <1489..1670
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..1971
                     /gene="LOC144114790"
                     /coded_by="XM_077648750.1:449..6364"
                     /db_xref="GeneID:144114790"
ORIGIN      
        1 mreqqdvagl slgqarrlve airkiksqkq rpslerishv vglradlvdr qleqaitrgf
       61 ilkvmnkgqc sykdaetlkl avkkkcaats lvsstaaggg raraaavaaa aaigagihrr
      121 kdlmhsavea vralgekggs tfpaivkfvr qalrmdpgir lrlaikravs aghllrvgrl
      181 yqlpcdesrs esdsddevgt sdrlgagkng dlagrtghkr rsalqgrcsv chgmprnnks
      241 gvaeellsch sctfsahpsc lkhmkelavv rlnskwqcpq crtcsrcgtr keeehllcce
      301 vcdshfhlgc lkppllkapk gswkcascss rrlksinfvn nlaskikqrn kryhngmmrk
      361 qriptlkdik qvaslratqh ancfapsrss skkeipkstm rstrdtpsdl ppsvsekdlk
      421 ifkktqeral kemgqdlvvp dhqtrcpaai efgqyeiqtw ysspypqeya rlpklflcef
      481 clkymksrni lsrhmrkctw fhppateiyr kgevsvfevd gnvskiycqn lcllaklfld
      541 hktlyydvep flfyvltknd tkgchlvgyf skekhcqqry nvscimtmpq yqrqgfgrfl
      601 idfsyllsrk egltgtpekp lsdlgrisym sywksillef lnnyketnis iqclssstgl
      661 nihdiattlq hlnmfkkmgs eksrivirid kqmlsehmak vhsgkdrriv ldpeclrwtp
      721 lvtqpqfrde epeeeeqqqq qqqqqqqqqq qqqqqqqekq qqqrhrqret pvveevkekk
      781 rpklmslaas vaaeqrtrkq papvkrphrr rkrkipwlnr kparsaprvp qavsngkees
      841 gpetaavvqe vprpparklr rlsetakate pdaareeppa rrlrtkepaq aptataqvpr
      901 sasesvyphl ksgpghkgmv lppkksrrgv rligmepqeq qeapivdpvv kavtnlgaqe
      961 veqaatasss atqqqpvgat trrrssrves qpqlrprrtr svevskeeca ppvlapedap
     1021 lpavrasksp aaaddapkpp vleafengst gpatrrgtkr lkrvqrrgrp pkkqyipves
     1081 pkpdevpnte nsdtesgkle epaeerhlpv tteegsaspi pqqvssrpss pgdmavkeqi
     1141 teppaivvst etnseenevs idtkeqppdq dssahaevvs hsvqseedvy keestvvrta
     1201 kqdsgnsaal temdsaeaes aaspvettse eprdsmvveg tqhipdsalt svpetqseee
     1261 kesessvpkq qqesviegch feqspresgs hqdvpsvpec vdndlvdsle prpqitllps
     1321 daiannasvv stssqesqdi vvdnpspqvq mpptpitpqt pgsnppsqqt clsnqsscgv
     1381 eesaptgsyd smlghspasm mgcsphdlsm dasslkaapp vephryqepr evtrgprgep
     1441 metarmemmr pgrlaapskp dlsclgvytp dsssssvmss ggytpvdmeq lreagqlrev
     1501 nqlremnslr etnqlrdvsq lrdvnhlrea nqlrdmnqlr dvsqlrdvnh lrdinrlhdi
     1561 nqlremnqmr emnqmremnq mremnqmrea nqmrevsqmr evsqmrevsq mrevsqmrev
     1621 nqlrdinqlr dvsqlreasq lrdisqirev nrhevnqlrd mnqlrdplre ssplcdvnql
     1681 remgqrrldt paaslvagea qgalehlssp fadcthsrpp qahhrqqpqp hrqtpqqsyc
     1741 rpvtsaplsq gafvaaqaav aqaqaqasag vpgvmqsmvl mppasnymsi sgpgpapsyv
     1801 mgvpvggmma asgppqrgfs hgyaygpgsn ascslaklqq ltngimdiap gspgqsaaaa
     1861 aaaaaacamt pptapaasra lsqsygspta appagsakya pryqrgplls gyqgalngyr
     1921 qgtptgpylq qpapmqmvqp qyqepsqnpm ytygylsgsl ppqtlnsmmr r
//
LOCUS       XP_077504877            1971 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114790 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504877
VERSION     XP_077504877.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648751.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1971
                     /product="uncharacterized protein LOC144114790 isoform X2"
                     /calculated_mol_wt=218346
     Region          128..178
                     /region_name="H15"
                     /note="Domain in histone families 1 and 5; smart00526"
                     /db_xref="CDD:197772"
     Site            159..170
                     /site_type="DNA binding"
                     /note="DNA-binding site [nucleotide binding]"
                     /db_xref="CDD:238028"
     Region          228..281
                     /region_name="PHD_SF"
                     /note="PHD finger superfamily; cl22851"
                     /db_xref="CDD:473978"
     Site            order(246..249,253,276)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:276966"
     Region          283..328
                     /region_name="PHD_SF"
                     /note="PHD finger superfamily; cl22851"
                     /db_xref="CDD:473978"
     Site            order(283,296..299,303,323)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:276966"
     Region          <448..725
                     /region_name="PLN00104"
                     /note="MYST -like histone acetyltransferase; Provisional"
                     /db_xref="CDD:215056"
     Region          <780..1173
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          <1489..1670
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..1971
                     /gene="LOC144114790"
                     /coded_by="XM_077648751.1:266..6181"
                     /db_xref="GeneID:144114790"
ORIGIN      
        1 mreqqdvagl slgqarrlve airkiksqkq rpslerishv vglradlvdr qleqaitrgf
       61 ilkvmnkgqc sykdaetlkl avkkkcaats lvsstaaggg raraaavaaa aaigagihrr
      121 kdlmhsavea vralgekggs tfpaivkfvr qalrmdpgir lrlaikravs aghllrvgrl
      181 yqlpcdesrs esdsddevgt sdrlgagkng dlagrtghkr rsalqgrcsv chgmprnnks
      241 gvaeellsch sctfsahpsc lkhmkelavv rlnskwqcpq crtcsrcgtr keeehllcce
      301 vcdshfhlgc lkppllkapk gswkcascss rrlksinfvn nlaskikqrn kryhngmmrk
      361 qriptlkdik qvaslratqh ancfapsrss skkeipkstm rstrdtpsdl ppsvsekdlk
      421 ifkktqeral kemgqdlvvp dhqtrcpaai efgqyeiqtw ysspypqeya rlpklflcef
      481 clkymksrni lsrhmrkctw fhppateiyr kgevsvfevd gnvskiycqn lcllaklfld
      541 hktlyydvep flfyvltknd tkgchlvgyf skekhcqqry nvscimtmpq yqrqgfgrfl
      601 idfsyllsrk egltgtpekp lsdlgrisym sywksillef lnnyketnis iqclssstgl
      661 nihdiattlq hlnmfkkmgs eksrivirid kqmlsehmak vhsgkdrriv ldpeclrwtp
      721 lvtqpqfrde epeeeeqqqq qqqqqqqqqq qqqqqqqekq qqqrhrqret pvveevkekk
      781 rpklmslaas vaaeqrtrkq papvkrphrr rkrkipwlnr kparsaprvp qavsngkees
      841 gpetaavvqe vprpparklr rlsetakate pdaareeppa rrlrtkepaq aptataqvpr
      901 sasesvyphl ksgpghkgmv lppkksrrgv rligmepqeq qeapivdpvv kavtnlgaqe
      961 veqaatasss atqqqpvgat trrrssrves qpqlrprrtr svevskeeca ppvlapedap
     1021 lpavrasksp aaaddapkpp vleafengst gpatrrgtkr lkrvqrrgrp pkkqyipves
     1081 pkpdevpnte nsdtesgkle epaeerhlpv tteegsaspi pqqvssrpss pgdmavkeqi
     1141 teppaivvst etnseenevs idtkeqppdq dssahaevvs hsvqseedvy keestvvrta
     1201 kqdsgnsaal temdsaeaes aaspvettse eprdsmvveg tqhipdsalt svpetqseee
     1261 kesessvpkq qqesviegch feqspresgs hqdvpsvpec vdndlvdsle prpqitllps
     1321 daiannasvv stssqesqdi vvdnpspqvq mpptpitpqt pgsnppsqqt clsnqsscgv
     1381 eesaptgsyd smlghspasm mgcsphdlsm dasslkaapp vephryqepr evtrgprgep
     1441 metarmemmr pgrlaapskp dlsclgvytp dsssssvmss ggytpvdmeq lreagqlrev
     1501 nqlremnslr etnqlrdvsq lrdvnhlrea nqlrdmnqlr dvsqlrdvnh lrdinrlhdi
     1561 nqlremnqmr emnqmremnq mremnqmrea nqmrevsqmr evsqmrevsq mrevsqmrev
     1621 nqlrdinqlr dvsqlreasq lrdisqirev nrhevnqlrd mnqlrdplre ssplcdvnql
     1681 remgqrrldt paaslvagea qgalehlssp fadcthsrpp qahhrqqpqp hrqtpqqsyc
     1741 rpvtsaplsq gafvaaqaav aqaqaqasag vpgvmqsmvl mppasnymsi sgpgpapsyv
     1801 mgvpvggmma asgppqrgfs hgyaygpgsn ascslaklqq ltngimdiap gspgqsaaaa
     1861 aaaaaacamt pptapaasra lsqsygspta appagsakya pryqrgplls gyqgalngyr
     1921 qgtptgpylq qpapmqmvqp qyqepsqnpm ytygylsgsl ppqtlnsmmr r
//
LOCUS       XP_077504878            1971 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114790 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504878
VERSION     XP_077504878.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648752.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1971
                     /product="uncharacterized protein LOC144114790 isoform X2"
                     /calculated_mol_wt=218346
     Region          128..178
                     /region_name="H15"
                     /note="Domain in histone families 1 and 5; smart00526"
                     /db_xref="CDD:197772"
     Site            159..170
                     /site_type="DNA binding"
                     /note="DNA-binding site [nucleotide binding]"
                     /db_xref="CDD:238028"
     Region          228..281
                     /region_name="PHD_SF"
                     /note="PHD finger superfamily; cl22851"
                     /db_xref="CDD:473978"
     Site            order(246..249,253,276)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:276966"
     Region          283..328
                     /region_name="PHD_SF"
                     /note="PHD finger superfamily; cl22851"
                     /db_xref="CDD:473978"
     Site            order(283,296..299,303,323)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:276966"
     Region          <448..725
                     /region_name="PLN00104"
                     /note="MYST -like histone acetyltransferase; Provisional"
                     /db_xref="CDD:215056"
     Region          <780..1173
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          <1489..1670
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..1971
                     /gene="LOC144114790"
                     /coded_by="XM_077648752.1:261..6176"
                     /db_xref="GeneID:144114790"
ORIGIN      
        1 mreqqdvagl slgqarrlve airkiksqkq rpslerishv vglradlvdr qleqaitrgf
       61 ilkvmnkgqc sykdaetlkl avkkkcaats lvsstaaggg raraaavaaa aaigagihrr
      121 kdlmhsavea vralgekggs tfpaivkfvr qalrmdpgir lrlaikravs aghllrvgrl
      181 yqlpcdesrs esdsddevgt sdrlgagkng dlagrtghkr rsalqgrcsv chgmprnnks
      241 gvaeellsch sctfsahpsc lkhmkelavv rlnskwqcpq crtcsrcgtr keeehllcce
      301 vcdshfhlgc lkppllkapk gswkcascss rrlksinfvn nlaskikqrn kryhngmmrk
      361 qriptlkdik qvaslratqh ancfapsrss skkeipkstm rstrdtpsdl ppsvsekdlk
      421 ifkktqeral kemgqdlvvp dhqtrcpaai efgqyeiqtw ysspypqeya rlpklflcef
      481 clkymksrni lsrhmrkctw fhppateiyr kgevsvfevd gnvskiycqn lcllaklfld
      541 hktlyydvep flfyvltknd tkgchlvgyf skekhcqqry nvscimtmpq yqrqgfgrfl
      601 idfsyllsrk egltgtpekp lsdlgrisym sywksillef lnnyketnis iqclssstgl
      661 nihdiattlq hlnmfkkmgs eksrivirid kqmlsehmak vhsgkdrriv ldpeclrwtp
      721 lvtqpqfrde epeeeeqqqq qqqqqqqqqq qqqqqqqekq qqqrhrqret pvveevkekk
      781 rpklmslaas vaaeqrtrkq papvkrphrr rkrkipwlnr kparsaprvp qavsngkees
      841 gpetaavvqe vprpparklr rlsetakate pdaareeppa rrlrtkepaq aptataqvpr
      901 sasesvyphl ksgpghkgmv lppkksrrgv rligmepqeq qeapivdpvv kavtnlgaqe
      961 veqaatasss atqqqpvgat trrrssrves qpqlrprrtr svevskeeca ppvlapedap
     1021 lpavrasksp aaaddapkpp vleafengst gpatrrgtkr lkrvqrrgrp pkkqyipves
     1081 pkpdevpnte nsdtesgkle epaeerhlpv tteegsaspi pqqvssrpss pgdmavkeqi
     1141 teppaivvst etnseenevs idtkeqppdq dssahaevvs hsvqseedvy keestvvrta
     1201 kqdsgnsaal temdsaeaes aaspvettse eprdsmvveg tqhipdsalt svpetqseee
     1261 kesessvpkq qqesviegch feqspresgs hqdvpsvpec vdndlvdsle prpqitllps
     1321 daiannasvv stssqesqdi vvdnpspqvq mpptpitpqt pgsnppsqqt clsnqsscgv
     1381 eesaptgsyd smlghspasm mgcsphdlsm dasslkaapp vephryqepr evtrgprgep
     1441 metarmemmr pgrlaapskp dlsclgvytp dsssssvmss ggytpvdmeq lreagqlrev
     1501 nqlremnslr etnqlrdvsq lrdvnhlrea nqlrdmnqlr dvsqlrdvnh lrdinrlhdi
     1561 nqlremnqmr emnqmremnq mremnqmrea nqmrevsqmr evsqmrevsq mrevsqmrev
     1621 nqlrdinqlr dvsqlreasq lrdisqirev nrhevnqlrd mnqlrdplre ssplcdvnql
     1681 remgqrrldt paaslvagea qgalehlssp fadcthsrpp qahhrqqpqp hrqtpqqsyc
     1741 rpvtsaplsq gafvaaqaav aqaqaqasag vpgvmqsmvl mppasnymsi sgpgpapsyv
     1801 mgvpvggmma asgppqrgfs hgyaygpgsn ascslaklqq ltngimdiap gspgqsaaaa
     1861 aaaaaacamt pptapaasra lsqsygspta appagsakya pryqrgplls gyqgalngyr
     1921 qgtptgpylq qpapmqmvqp qyqepsqnpm ytygylsgsl ppqtlnsmmr r
//
LOCUS       XP_077504879             358 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114791 [Amblyomma americanum].
ACCESSION   XP_077504879
VERSION     XP_077504879.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648753.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..358
                     /product="uncharacterized protein LOC144114791"
                     /calculated_mol_wt=37224
     Region          <60..321
                     /region_name="MARTX_Nterm"
                     /note="MARTX multifunctional-autoprocessing
                     repeats-in-toxin holotoxin N-terminal region; NF012221"
                     /db_xref="CDD:467957"
     CDS             1..358
                     /gene="LOC144114791"
                     /coded_by="XM_077648753.1:88..1164"
                     /db_xref="GeneID:144114791"
ORIGIN      
        1 mssskqkava asykhqktaa nskqqeasrk qkkaasnskk qaaissmqqa alcskhqpps
       61 snrqeaanis kqqaaahskq kagisskrle paaanskqqe paanskhraa easskhqaps
      121 ssskqqaaar skpqaadskh haaeasskhq apstkqqqqt assskkqaas sskkqaqars
      181 rqeaaacsrk qqapcsrnkq qaaptssskk qqqaaksssk qhapcsrseq qagrastyqq
      241 qqtasnskqq aaersqqqaa atssskqqqq ppaaastkqq qqtasskeqa aaassskqpa
      301 aassskqqqa panskqqaaa asspklqqea crkqqaaars kqqaaagssk nqaaanss
//
LOCUS       XP_077504880            1819 aa            linear   INV 25-NOV-2025
DEFINITION  cadherin 74A isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504880
VERSION     XP_077504880.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648754.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1819
                     /product="cadherin 74A isoform X1"
                     /calculated_mol_wt=200173
     Region          44..134
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(48..49,94,96,130,132..133)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          142..250
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(149..150,213,215,247,249..250)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          259..373
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(266..267,327,329,369,371..372)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          382..489
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(388..389,452,454,485,487..488)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          497..595
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(504..505,560,562,591,593..594)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          603..707
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(610..611,667,669,704,706..707)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          716..812
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(723..724,776,778,810,812)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          822..931
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          951..1051
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(957..958,1010,1012,1047,1049..1050)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1059..1161
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1066..1067,1121,1123,1157,1159..1160)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1170..1270
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1176..1177,1232,1235,1266,1268..1269)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1284..1385
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1289..1290,1348,1350,1381,1383..1384)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1402..1507
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1404..1405,1460,1462,1503,1505..1506)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1515..1621
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1522..1523,1585,1587,1618,1620..1621)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     CDS             1..1819
                     /gene="Cad74A"
                     /coded_by="XM_077648754.1:711..6170"
                     /db_xref="GeneID:144114792"
ORIGIN      
        1 mteatsfaar liwrwltifw vigkcqgqtn appeflvggn mdkfslredt pvgasvytlr
       61 aidpehtkvs yyisggtfsv nkdtgvvrlt rpldreeest vdviisitde kiqgqkantv
      121 slqrevnild yndnppvfen gpytfhvded tqvgttlyrn ivvtdddlgi nadvevtcqr
      181 nltpvacdlf glnqvrlgmg rfiqeitlkn pldyeeqtty vmslfandla menrmnstad
      241 viikvndvqd qppvfvadsy svtvaenspe gtsvllasah dadagfrrll sfklvndtkg
      301 yfrlgkvdvd dngihhvvve tsnvtidred edieksggly sfqlqavemv ngvpkgevav
      361 sdvnivitdt ddhvpefssr nfsvvipedt pensaipgln mvvndndvge narfglhlrn
      421 innsdgvfsv yplsavgrtp vlikvadasr ldyedenart fvfqvvasrh gfevasatvt
      481 vslsdandng pmfeneqyli qvpesarngm tifsleayda dsgpfgklsy smkgygsekf
      541 vvekdtgrik iatckngcld yesvpnysft yeaqdgggkv ssvnlfiqvv dvndnapkft
      601 kdsylvdlve datefsspvf vkatdadgpn qgngrvqymi katnlthpev isidaetgel
      661 sltgslnvta apygatrfev iiqatdfgep rlaseipfii rlkrknegap yflnapykas
      721 vnenakpgtt vlqlvatdpd gpdgnisyyi dsgardnfvl nkltgilsts snanldreln
      781 gdsyhilvya vdggvvpqka fttvtvsvqd vnnkppvfak dsyvhwvtes lpvgrevlnv
      841 tavdpdvgak vrydieepii ardktgslvt stitynykna fringvtgqi tvnspldyna
      901 asvitfnvaa vdqngalqpq kttvevtiyi qahsesnpif appwtpanpk ieitvpeest
      961 vgstiftvsa rdplthaavt nfakipesdq gsffsvspis gavtlnqrld yeelpekvla
     1021 fdvkaiigqd knnqkssiat viinvqdind nspvfsqnsy vtmvseatqy phtillitat
     1081 dkdtqqgygv vryyvsgegs dvfsinettg algiqegail drekqplynl qvtavdnpga
     1141 psnqrrtsvl vvikiqdend napeftqsgy tavvpenvpt gfsiltvran dkdagsnsei
     1201 sysfvdepem gaitlfaind ktgvitviqp lsgrgrsepy fltvratdgg spslhtdvki
     1261 liiigdvssn dgiprfirpr mgevayvhen vtigasvfqv qavdpdsals sngqvaykfl
     1321 dkhsavgedl efeidpgsgl vsttavldre krdnytlvvv aydmgvppqe ahqilrvvvl
     1381 dvddnepefd rstdaepvvm kikeeipigt ivgkvrafda digtnafidy yivggnmdnv
     1441 ftiqrtgnne geiltkrrvd reqkdryllt vqvsmpskrl eqvdatynpd dktqllvdik
     1501 vediddnsps fekpsyvlgi rinseinsel itlkardpds ssnmimysir nvtyyrpssk
     1561 esmpvmgqfe ldahkgslrn vrslgkyrdg yfditvecqs apgpqntavt hvrvhilhds
     1621 dlmkfifykq pqeirkvipq fekdlnqala savtaniydt qystredgsl dfesasscfq
     1681 llenglvvsp dvvakmldms sshkvqalyh nysivgiekc tpgrnpykmk wteicvliva
     1741 cliavmgfil tvilcfvras yknnikrklk qrgprrlceq tpavsmislp shqriyewqe
     1801 snrapaldam sfrsyptmr
//
LOCUS       XP_077504881            1826 aa            linear   INV 25-NOV-2025
DEFINITION  cadherin 74A isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504881
VERSION     XP_077504881.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648755.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1826
                     /product="cadherin 74A isoform X2"
                     /calculated_mol_wt=200987
     Region          51..141
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(55..56,101,103,137,139..140)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          149..257
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(156..157,220,222,254,256..257)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          266..380
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(273..274,334,336,376,378..379)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          389..496
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(395..396,459,461,492,494..495)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          504..602
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(511..512,567,569,598,600..601)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          610..714
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(617..618,674,676,711,713..714)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          723..819
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(730..731,783,785,817,819)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          834..938
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          958..1058
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(964..965,1017,1019,1054,1056..1057)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1066..1168
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1073..1074,1128,1130,1164,1166..1167)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1177..1277
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1183..1184,1239,1242,1273,1275..1276)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1291..1392
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1296..1297,1355,1357,1388,1390..1391)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1409..1514
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1411..1412,1467,1469,1510,1512..1513)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1522..1628
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1529..1530,1592,1594,1625,1627..1628)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     CDS             1..1826
                     /gene="Cad74A"
                     /coded_by="XM_077648755.1:468..5948"
                     /db_xref="GeneID:144114792"
ORIGIN      
        1 mteatsfaar liwrwltifw vivylctfsg kcqgqtnapp eflvggnmdk fslredtpvg
       61 asvytlraid pehtkvsyyi sggtfsvnkd tgvvrltrpl dreeestvdv iisitdekiq
      121 gqkantvslq revnildynd nppvfengpy tfhvdedtqv gttlyrnivv tdddlginad
      181 vevtcqrnlt pvacdlfgln qvrlgmgrfi qeitlknpld yeeqttyvms lfandlamen
      241 rmnstadvii kvndvqdqpp vfvadsysvt vaenspegts vllasahdad agfrrllsfk
      301 lvndtkgyfr lgkvdvddng ihhvvvetsn vtidrededi eksgglysfq lqavemvngv
      361 pkgevavsdv nivitdtddh vpefssrnfs vvipedtpen saipglnmvv ndndvgenar
      421 fglhlrninn sdgvfsvypl savgrtpvli kvadasrldy edenartfvf qvvasrhgfe
      481 vasatvtvsl sdandngpmf eneqyliqvp esarngmtif sleaydadsg pfgklsysmk
      541 gygsekfvve kdtgrikiat ckngcldyes vpnysftyea qdgggkvssv nlfiqvvdvn
      601 dnapkftkds ylvdlvedat efsspvfvka tdadgpnqgn grvqymikat nlthpevisi
      661 daetgelslt gslnvtaapy gatrfeviiq atdfgeprla seipfiirlk rknegapyfl
      721 napykasvne nakpgttvlq lvatdpdgpd gnisyyidsg ardnfvlnkl tgilstssna
      781 nldrelngds yhilvyavdg gvvpqkaftt vtvsvqdvnn kppvfakdsy vhwvteslpv
      841 grevlnvtav dpdvgakvry dieepiiard ktgslvtsti tynyknafri ngvtgqitvn
      901 spldynaasv itfnvaavdq ngalqpqktt vevtiyiqah sesnpifapp wtpanpkiei
      961 tvpeestvgs tiftvsardp lthaavtnfa kipesdqgsf fsvspisgav tlnqrldyee
     1021 lpekvlafdv kaiigqdknn qkssiatvii nvqdindnsp vfsqnsyvtm vseatqypht
     1081 illitatdkd tqqgygvvry yvsgegsdvf sinettgalg iqegaildre kqplynlqvt
     1141 avdnpgapsn qrrtsvlvvi kiqdendnap eftqsgytav vpenvptgfs iltvrandkd
     1201 agsnseisys fvdepemgai tlfaindktg vitviqplsg rgrsepyflt vratdggsps
     1261 lhtdvkilii igdvssndgi prfirprmge vayvhenvti gasvfqvqav dpdsalssng
     1321 qvaykfldkh savgedlefe idpgsglvst tavldrekrd nytlvvvayd mgvppqeahq
     1381 ilrvvvldvd dnepefdrst daepvvmkik eeipigtivg kvrafdadig tnafidyyiv
     1441 ggnmdnvfti qrtgnnegei ltkrrvdreq kdrylltvqv smpskrleqv datynpddkt
     1501 qllvdikved iddnspsfek psyvlgirin seinselitl kardpdsssn mimysirnvt
     1561 yyrpsskesm pvmgqfelda hkgslrnvrs lgkyrdgyfd itvecqsapg pqntavthvr
     1621 vhilhdsdlm kfifykqpqe irkvipqfek dlnqalasav taniydtqys tredgsldfe
     1681 sasscfqlle nglvvspdvv akmldmsssh kvqalyhnys ivgiekctpg rnpykmkwte
     1741 icvlivacli avmgfiltvi lcfvrasykn nikrklkqrg prrlceqtpa vsmislpshq
     1801 riyewqesnr apaldamsfr syptmr
//
LOCUS       XP_077504882             124 aa            linear   INV 25-NOV-2025
DEFINITION  prefoldin subunit 1 [Amblyomma americanum].
ACCESSION   XP_077504882
VERSION     XP_077504882.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648756.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..124
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..124
                     /product="prefoldin subunit 1"
                     /calculated_mol_wt=14121
     Region          16..114
                     /region_name="Prefoldin_1"
                     /note="prefoldin subunit 1; cd23164"
                     /db_xref="CDD:467480"
     Site            order(16..17,20,27,30,91,94..95,97..98,100..102,104..105,
                     108..109,111..112)
                     /site_type="other"
                     /note="prefoldin beta subunit /TRiC interface [polypeptide
                     binding]"
                     /db_xref="CDD:467480"
     Region          16..55
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:467480"
     Site            order(51..52,55,57..58,60..62,64,69)
                     /site_type="other"
                     /note="prefoldin oligomer interface [polypeptide binding]"
                     /db_xref="CDD:467480"
     Region          75..114
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:467480"
     CDS             1..124
                     /gene="Pfdn1"
                     /coded_by="XM_077648756.1:53..427"
                     /db_xref="GeneID:144114793"
ORIGIN      
        1 maatpvdmel kkafqelqik midttrrlri admqieslrk avrhayctge vlseapsgtk
       61 myqsvgrmfv aadfddimss ldvkmktcde kikslennka ylekslkese nnlremiiqk
      121 qsmh
//
LOCUS       XP_077504883             803 aa            linear   INV 25-NOV-2025
DEFINITION  follistatin-related protein 5-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504883
VERSION     XP_077504883.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648757.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..803
                     /product="follistatin-related protein 5-like isoform X1"
                     /calculated_mol_wt=89529
     Region          20..65
                     /region_name="KAZAL"
                     /note="Kazal type serine protease inhibitors; smart00280"
                     /db_xref="CDD:197624"
     Region          149..204
                     /region_name="EFh"
                     /note="EF-hand, calcium binding motif; A diverse
                     superfamily of calcium sensors and calcium signal
                     modulators; most examples in this alignment model have 2
                     active canonical EF hands. Ca2+ binding induces a
                     conformational change in the EF-hand motif, leading to...;
                     cl08302"
                     /db_xref="CDD:415501"
     Site            order(154,156,158,165,192,194,196,203)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238008"
     Region          222..292
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          310..401
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          327..331
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          340..344
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          364..368
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          378..383
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          393..396
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..803
                     /gene="LOC144114794"
                     /coded_by="XM_077648757.1:395..2806"
                     /db_xref="GeneID:144114794"
ORIGIN      
        1 mvcgrgrqce lneageascv cqksckkrrk pvcgsdgnyy inhcelhraa clagkpiaid
       61 hrgncnrveh asstektnst qagtdskmer temppheakv atttesedst qqdctqqeyd
      121 fmkenlllyn nakmtdnkkt gneylvsimf shydhnhngl leaeelwkag eedrlgqlst
      181 vciladmllf ddanhdgrln inefylafsk lysvsvvsld kslevnhvta rvgdnveikc
      241 dvtgtppppi vwrrnnmdls slsedeikvf vdgslyltnv qlvhsgnytc haqrnhdviq
      301 thilhvqtmp evrvipklqs rapgelaeme chvtgvptpr vtwlkndegl rvatdkytii
      361 gngtalmvgk itysdtgaym cvasnpagst rdisslvvqd qptptapsee rrfftfhdwg
      421 vavydpdncr lyhqiqsvdi ipgtqeyvcg drgancswgr ainvadryvy vsqptkgrvl
      481 iisriqmvvv dvvvtdkfpv dlhyvphldq vwvlcwrgpv drgtktiqii rdasqkkkhh
      541 tvhpepvdgh fdlvsglfip paqdlgypwk ygyvvhtnqr gmykmdlram kyiktvdltp
      601 yncapasadf aslggfvile clepitqrpm gqilldyltd tvlshktnlf grprvspdsr
      661 hvitlqsann svvivaqevt eqglrflfdv kttlqisdat fftsrtshsy dlyassstke
      721 dillvnlqdg kvevitgtgr aidprgarwg nanrpissag vfgtymvtta seavfvlngk
      781 trtvnceign vvhprmmvwv rsp
//
LOCUS       XP_077504884             799 aa            linear   INV 25-NOV-2025
DEFINITION  follistatin-related protein 5-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504884
VERSION     XP_077504884.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648758.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..799
                     /product="follistatin-related protein 5-like isoform X2"
                     /calculated_mol_wt=89037
     Region          20..65
                     /region_name="KAZAL"
                     /note="Kazal type serine protease inhibitors; smart00280"
                     /db_xref="CDD:197624"
     Region          149..204
                     /region_name="EFh"
                     /note="EF-hand, calcium binding motif; A diverse
                     superfamily of calcium sensors and calcium signal
                     modulators; most examples in this alignment model have 2
                     active canonical EF hands. Ca2+ binding induces a
                     conformational change in the EF-hand motif, leading to...;
                     cl08302"
                     /db_xref="CDD:415501"
     Site            order(154,156,158,165,192,194,196,203)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238008"
     Region          218..288
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          306..397
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          323..327
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          336..340
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          360..364
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          374..379
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          389..392
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..799
                     /gene="LOC144114794"
                     /coded_by="XM_077648758.1:407..2806"
                     /db_xref="GeneID:144114794"
ORIGIN      
        1 mvcgrgrqce lneageascv cqksckkrrk pvcgsdgnyy inhcelhraa clagkpiaid
       61 hrgncnrveh asstektnst qagtdskmer temppheakv atttesedst qqdctqqeyd
      121 fmkenlllyn nakmtdnkkt gneylvsimf shydhnhngl leaeelwkag eedrlgqlst
      181 vciladmllf ddanhdgrln inefylafsv svvsldksle vnhvtarvgd nveikcdvtg
      241 tppppivwrr nnmdlsslse deikvfvdgs lyltnvqlvh sgnytchaqr nhdviqthil
      301 hvqtmpevrv ipklqsrapg elaemechvt gvptprvtwl kndeglrvat dkytiigngt
      361 almvgkitys dtgaymcvas npagstrdis slvvqdqptp tapseerrff tfhdwgvavy
      421 dpdncrlyhq iqsvdiipgt qeyvcgdrga ncswgrainv adryvyvsqp tkgrvliisr
      481 iqmvvvdvvv tdkfpvdlhy vphldqvwvl cwrgpvdrgt ktiqiirdas qkkkhhtvhp
      541 epvdghfdlv sglfippaqd lgypwkygyv vhtnqrgmyk mdlramkyik tvdltpynca
      601 pasadfaslg gfvileclep itqrpmgqil ldyltdtvls hktnlfgrpr vspdsrhvit
      661 lqsannsvvi vaqevteqgl rflfdvkttl qisdatffts rtshsydlya ssstkedill
      721 vnlqdgkvev itgtgraidp rgarwgnanr pissagvfgt ymvttaseav fvlngktrtv
      781 nceignvvhp rmmvwvrsp
//
LOCUS       XP_077504885             620 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114797 [Amblyomma americanum].
ACCESSION   XP_077504885
VERSION     XP_077504885.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648759.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (DAPXPI010013077.1) annotated
            using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 244 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..620
                     /product="uncharacterized protein LOC144114797"
                     /calculated_mol_wt=65354
     Region          128..252
                     /region_name="PAX"
                     /note="Paired Box domain; smart00351"
                     /db_xref="CDD:128645"
     Site            order(133..134,139,141..143,145,150,162,164,173,176,
                     178..179,183,192..198,200..203,222..224,243,245..246,249,
                     252)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238076"
     CDS             1..620
                     /gene="LOC144114797"
                     /coded_by="XM_077648759.1:147..2009"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 244 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144114797"
ORIGIN      
        1 merlmmrssp snyndvklkl lqegglefrd admlypgdps aqvvsyaagp lqplpphedv
       61 ygstqlgaim dlqqqpqpqh vqaaqvqdhe pkkknangra tssnqsymdh prnsinvhad
      121 smqsddtghg gvnqlggvfv ngrplpdvvr qrivelahqg vrpcdisrql rvshgcvski
      181 lgryyetgsi rpgviggskp kvatpkvvda ianykkqnpt mfaweirdrl ladgvcdqdn
      241 ipsvssinri vrnkaaekak hhhvssnggs pqpqssptsv iahapasahe ssvsaaaaaa
      301 lqrpsysing ilgipsaeaa krkrddqvsq vhgdsardmn gdhgihedev krsraqynge
      361 qiysnlspnm wakwtkqdel kagmadlpts ngspyhhlts qasqpapppq ggpfeqafsp
      421 vvssadkqqa dydvamttin sqstavyspp lgsnsltplt pismqevksl vnsgasliet
      481 spnphsyhpa aaatgseiqg spspakaeap cplssltvlq pvqgaipgyg ssfsqysaap
      541 vtgsavagsa vagipgadya yaspytqyss aaaygaygyg ssgiinpsyy yahsartgsa
      601 gtglssagpl spnaykgerc
//
LOCUS       XP_077504886             657 aa            linear   INV 25-NOV-2025
DEFINITION  maternal embryonic leucine zipper kinase-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504886
VERSION     XP_077504886.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648760.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..657
                     /product="maternal embryonic leucine zipper kinase-like
                     isoform X1"
                     /calculated_mol_wt=72710
     Region          10..266
                     /region_name="STKc_MELK"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Maternal Embryonic Leucine zipper Kinase; cd14078"
                     /db_xref="CDD:270980"
     Site            order(20..21,23..26,28,41,43,56,73,90..92,96,98..99,101,
                     135,137,139..140,142,152..153,156,172..176,178,208..211)
                     /site_type="active"
                     /db_xref="CDD:270980"
     Site            order(20..21,23..26,28,41,43,56,73,90..92,96,139..140,142,
                     152..153)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270980"
     Site            order(96,98..99,101,135,137,139,156,172..176,178,208..211)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270980"
     Site            order(152..163,165..176)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270980"
     Region          284..335
                     /region_name="UBA_MELK"
                     /note="UBA domain found in maternal embryonic leucine
                     zipper kinase (MELK) and similar proteins; cd14341"
                     /db_xref="CDD:270526"
     Region          560..653
                     /region_name="MELK_C"
                     /note="C-terminal kinase associated domain 1 (KA1) of
                     Maternal embryonic leucine zipper kinase; cd12198"
                     /db_xref="CDD:213383"
     Site            order(560,563,633,635..636)
                     /site_type="other"
                     /note="putative phospholipid binding site [chemical
                     binding]"
                     /db_xref="CDD:213383"
     CDS             1..657
                     /gene="LOC144114798"
                     /coded_by="XM_077648760.1:268..2241"
                     /db_xref="GeneID:144114798"
ORIGIN      
        1 mpekpsvddl deqyilleti gtggfakvkl aihiltgekv avkimdkrsl gedlprvkle
       61 italkdlshq nicklyqvie tdtkiylvle ycpggelfdy ivekerlsee earhffrqiv
      121 savayihcqg yahrdlkpen llldenhnlk lidfglcakp kggmttslet ccgspayaap
      181 elvtgveyig smvdiwsmgv llyallcgcl pfddenvavl yrkiqlgeye cpewlsdssr
      241 qllsqmmtvi perrisvtql inhpwlvkgy acpvsiksty llsilddeav telavscgrs
      301 kssvaaelsq wrydymtaty lllilkksqg kvmrlafpdf yprermscsr klaleeppkg
      361 tpptsplrns lengldnaql lmlgsplkda anssyardla psakneqpts altqdingcd
      421 kenfaqprvp tprhkahkav qeaadagqqs spsgtqelmc tshaqalmsl asqnaanlsn
      481 qgtpvkksar apsadaefrp spmrtpdska tasaasfslv rgssakrvfg siekgldrvr
      541 lmltprrkgs staaaqdapr kvkvmqnvst mpavltpdqv ldslrtalfl kgiickqkry
      601 tlrgkirdew gkvqltfele vvqvpqpell girrkrltgd awhykrvcee vlkvraa
//
LOCUS       XP_077504888             648 aa            linear   INV 25-NOV-2025
DEFINITION  maternal embryonic leucine zipper kinase-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504888
VERSION     XP_077504888.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648762.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..648
                     /product="maternal embryonic leucine zipper kinase-like
                     isoform X2"
                     /calculated_mol_wt=71767
     Region          10..266
                     /region_name="STKc_MELK"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Maternal Embryonic Leucine zipper Kinase; cd14078"
                     /db_xref="CDD:270980"
     Site            order(20..21,23..26,28,41,43,56,73,90..92,96,98..99,101,
                     135,137,139..140,142,152..153,156,172..176,178,208..211)
                     /site_type="active"
                     /db_xref="CDD:270980"
     Site            order(20..21,23..26,28,41,43,56,73,90..92,96,139..140,142,
                     152..153)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270980"
     Site            order(96,98..99,101,135,137,139,156,172..176,178,208..211)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270980"
     Site            order(152..163,165..176)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270980"
     Region          284..335
                     /region_name="UBA_MELK"
                     /note="UBA domain found in maternal embryonic leucine
                     zipper kinase (MELK) and similar proteins; cd14341"
                     /db_xref="CDD:270526"
     Region          551..644
                     /region_name="MELK_C"
                     /note="C-terminal kinase associated domain 1 (KA1) of
                     Maternal embryonic leucine zipper kinase; cd12198"
                     /db_xref="CDD:213383"
     Site            order(551,554,624,626..627)
                     /site_type="other"
                     /note="putative phospholipid binding site [chemical
                     binding]"
                     /db_xref="CDD:213383"
     CDS             1..648
                     /gene="LOC144114798"
                     /coded_by="XM_077648762.1:138..2084"
                     /db_xref="GeneID:144114798"
ORIGIN      
        1 mpekpsvddl deqyilleti gtggfakvkl aihiltgekv avkimdkrsl gedlprvkle
       61 italkdlshq nicklyqvie tdtkiylvle ycpggelfdy ivekerlsee earhffrqiv
      121 savayihcqg yahrdlkpen llldenhnlk lidfglcakp kggmttslet ccgspayaap
      181 elvtgveyig smvdiwsmgv llyallcgcl pfddenvavl yrkiqlgeye cpewlsdssr
      241 qllsqmmtvi perrisvtql inhpwlvkgy acpvsiksty llsilddeav telavscgrs
      301 kssvaaelsq wrydymtaty lllilkksqg kvmrlafpdf yprermscsr klaleeppkg
      361 tpptsplrns lengldnaql lmlgsplkda anssyardla psakneqpts altqdingcd
      421 kenfaqprvp tprhkahkav qeaadagqqs spsgtqelms lasqnaanls nqgtpvkksa
      481 rapsadaefr pspmrtpdsk atasaasfsl vrgssakrvf gsiekgldrv rlmltprrkg
      541 sstaaaqdap rkvkvmqnvs tmpavltpdq vldslrtalf lkgiickqkr ytlrgkirde
      601 wgkvqltfel evvqvpqpel lgirrkrltg dawhykrvce evlkvraa
//
LOCUS       XP_077504889             633 aa            linear   INV 25-NOV-2025
DEFINITION  maternal embryonic leucine zipper kinase-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504889
VERSION     XP_077504889.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648763.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..633
                     /product="maternal embryonic leucine zipper kinase-like
                     isoform X3"
                     /calculated_mol_wt=70233
     Region          10..266
                     /region_name="STKc_MELK"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Maternal Embryonic Leucine zipper Kinase; cd14078"
                     /db_xref="CDD:270980"
     Site            order(20..21,23..26,28,41,43,56,73,90..92,96,98..99,101,
                     135,137,139..140,142,152..153,156,172..176,178,208..211)
                     /site_type="active"
                     /db_xref="CDD:270980"
     Site            order(20..21,23..26,28,41,43,56,73,90..92,96,139..140,142,
                     152..153)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270980"
     Site            order(96,98..99,101,135,137,139,156,172..176,178,208..211)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270980"
     Site            order(152..163,165..176)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270980"
     Region          284..335
                     /region_name="UBA_MELK"
                     /note="UBA domain found in maternal embryonic leucine
                     zipper kinase (MELK) and similar proteins; cd14341"
                     /db_xref="CDD:270526"
     Region          536..629
                     /region_name="MELK_C"
                     /note="C-terminal kinase associated domain 1 (KA1) of
                     Maternal embryonic leucine zipper kinase; cd12198"
                     /db_xref="CDD:213383"
     Site            order(536,539,609,611..612)
                     /site_type="other"
                     /note="putative phospholipid binding site [chemical
                     binding]"
                     /db_xref="CDD:213383"
     CDS             1..633
                     /gene="LOC144114798"
                     /coded_by="XM_077648763.1:248..2149"
                     /db_xref="GeneID:144114798"
ORIGIN      
        1 mpekpsvddl deqyilleti gtggfakvkl aihiltgekv avkimdkrsl gedlprvkle
       61 italkdlshq nicklyqvie tdtkiylvle ycpggelfdy ivekerlsee earhffrqiv
      121 savayihcqg yahrdlkpen llldenhnlk lidfglcakp kggmttslet ccgspayaap
      181 elvtgveyig smvdiwsmgv llyallcgcl pfddenvavl yrkiqlgeye cpewlsdssr
      241 qllsqmmtvi perrisvtql inhpwlvkgy acpvsiksty llsilddeav telavscgrs
      301 kssvaaelsq wrydymtaty lllilkksqg kvmrlafpdf yprermscsr klaleeppkg
      361 tpptsplrns lengldnaql lmlgsplkda anssyardla psakneqpts altqdingcd
      421 kenfaqprvp tprhkahkav qeaaalasqn aanlsnqgtp vkksarapsa daefrpspmr
      481 tpdskatasa asfslvrgss akrvfgsiek gldrvrlmlt prrkgsstaa aqdaprkvkv
      541 mqnvstmpav ltpdqvldsl rtalflkgii ckqkrytlrg kirdewgkvq ltfelevvqv
      601 pqpellgirr krltgdawhy krvceevlkv raa
//
LOCUS       XP_077504890             628 aa            linear   INV 25-NOV-2025
DEFINITION  maternal embryonic leucine zipper kinase-like isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077504890
VERSION     XP_077504890.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648764.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..628
                     /product="maternal embryonic leucine zipper kinase-like
                     isoform X4"
                     /calculated_mol_wt=69749
     Region          10..266
                     /region_name="STKc_MELK"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Maternal Embryonic Leucine zipper Kinase; cd14078"
                     /db_xref="CDD:270980"
     Site            order(20..21,23..26,28,41,43,56,73,90..92,96,98..99,101,
                     135,137,139..140,142,152..153,156,172..176,178,208..211)
                     /site_type="active"
                     /db_xref="CDD:270980"
     Site            order(20..21,23..26,28,41,43,56,73,90..92,96,139..140,142,
                     152..153)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270980"
     Site            order(96,98..99,101,135,137,139,156,172..176,178,208..211)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270980"
     Site            order(152..163,165..176)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270980"
     Region          531..624
                     /region_name="MELK_C"
                     /note="C-terminal kinase associated domain 1 (KA1) of
                     Maternal embryonic leucine zipper kinase; cd12198"
                     /db_xref="CDD:213383"
     Site            order(531,534,604,606..607)
                     /site_type="other"
                     /note="putative phospholipid binding site [chemical
                     binding]"
                     /db_xref="CDD:213383"
     CDS             1..628
                     /gene="LOC144114798"
                     /coded_by="XM_077648764.1:138..2024"
                     /db_xref="GeneID:144114798"
ORIGIN      
        1 mpekpsvddl deqyilleti gtggfakvkl aihiltgekv avkimdkrsl gedlprvkle
       61 italkdlshq nicklyqvie tdtkiylvle ycpggelfdy ivekerlsee earhffrqiv
      121 savayihcqg yahrdlkpen llldenhnlk lidfglcakp kggmttslet ccgspayaap
      181 elvtgveyig smvdiwsmgv llyallcgcl pfddenvavl yrkiqlgeye cpewlsdssr
      241 qllsqmmtvi perrisvtql inhpwlvkgy acpvsiksty lwrydymtat ylllilkksq
      301 gkvmrlafpd fyprermscs rklaleeppk gtpptsplrn slengldnaq llmlgsplkd
      361 aanssyardl apsakneqpt saltqdingc dkenfaqprv ptprhkahka vqeaadagqq
      421 sspsgtqelm ctshaqalms lasqnaanls nqgtpvkksa rapsadaefr pspmrtpdsk
      481 atasaasfsl vrgssakrvf gsiekgldrv rlmltprrkg sstaaaqdap rkvkvmqnvs
      541 tmpavltpdq vldslrtalf lkgiickqkr ytlrgkirde wgkvqltfel evvqvpqpel
      601 lgirrkrltg dawhykrvce evlkvraa
//
LOCUS       XP_077504891             567 aa            linear   INV 25-NOV-2025
DEFINITION  maternal embryonic leucine zipper kinase-like isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077504891
VERSION     XP_077504891.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648765.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..567
                     /product="maternal embryonic leucine zipper kinase-like
                     isoform X5"
                     /calculated_mol_wt=62292
     Region          10..266
                     /region_name="STKc_MELK"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Maternal Embryonic Leucine zipper Kinase; cd14078"
                     /db_xref="CDD:270980"
     Site            order(20..21,23..26,28,41,43,56,73,90..92,96,98..99,101,
                     135,137,139..140,142,152..153,156,172..176,178,208..211)
                     /site_type="active"
                     /db_xref="CDD:270980"
     Site            order(20..21,23..26,28,41,43,56,73,90..92,96,139..140,142,
                     152..153)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270980"
     Site            order(96,98..99,101,135,137,139,156,172..176,178,208..211)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270980"
     Site            order(152..163,165..176)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270980"
     Region          284..335
                     /region_name="UBA_MELK"
                     /note="UBA domain found in maternal embryonic leucine
                     zipper kinase (MELK) and similar proteins; cd14341"
                     /db_xref="CDD:270526"
     CDS             1..567
                     /gene="LOC144114798"
                     /coded_by="XM_077648765.1:138..1841"
                     /db_xref="GeneID:144114798"
ORIGIN      
        1 mpekpsvddl deqyilleti gtggfakvkl aihiltgekv avkimdkrsl gedlprvkle
       61 italkdlshq nicklyqvie tdtkiylvle ycpggelfdy ivekerlsee earhffrqiv
      121 savayihcqg yahrdlkpen llldenhnlk lidfglcakp kggmttslet ccgspayaap
      181 elvtgveyig smvdiwsmgv llyallcgcl pfddenvavl yrkiqlgeye cpewlsdssr
      241 qllsqmmtvi perrisvtql inhpwlvkgy acpvsiksty llsilddeav telavscgrs
      301 kssvaaelsq wrydymtaty lllilkksqg kvmrlafpdf yprermscsr klaleeppkg
      361 tpptsplrns lengldnaql lmlgsplkda anssyardla psakneqpts altqdingcd
      421 kenfaqprvp tprhkahkav qeaadagqqs spsgtqelmc tshaqalmsl asqnaanlsn
      481 qgtpvkksar apsadaefrp spmrtpdska tasaasfslv rgssakrvfg siekgldrvr
      541 lmltprrkgs staaaqdapr kvkehss
//
LOCUS       XP_077504892             595 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114799 [Amblyomma americanum].
ACCESSION   XP_077504892
VERSION     XP_077504892.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648766.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..595
                     /product="uncharacterized protein LOC144114799"
                     /calculated_mol_wt=63932
     CDS             1..595
                     /gene="LOC144114799"
                     /coded_by="XM_077648766.1:14..1801"
                     /db_xref="GeneID:144114799"
ORIGIN      
        1 maavagtsgl ksfessstta kpavddddde lealrtaalm smkarekhkp nslnseprer
       61 pgayssysry hhsnqrampg kplfswsqht rpnliviqpv plegseaapp snrasiqtaq
      121 pssklllpqd rwcpqqgqds pasassgpsk rrgsgkfshf essseseesd ddllpsgsvs
      181 spsdpshghs sddeqnsacl splrdsldgc lsestsdllv sakwhemspe tgttdcarks
      241 gdvysalhts ghgssdgids giahapsqas gddsesgedv ddctakdllg lvsrqepsve
      301 dglknsavps envqelpvgs pglcgsrsgn dspvghmark spsrkeypqk hearltngvs
      361 ldvqkvhvap dsqredwfap lsreknssre gtpkstrsvd sqdravssvf earrrkfest
      421 vpvkpvggki vllkgqsktd elspchessp lpspraqars rsrstsppfl rsvlsvvkrg
      481 pgellpacae pkkrhrsgrt aevivhsvge essdasdria cssklpvhlr lgdpsmtrkk
      541 kskrkrsshd ssgysakrkh akgrcpdsdv rrtwlgpeqr rrrkcmpsah cdgap
//
LOCUS       XP_077504893             595 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114799 [Amblyomma americanum].
ACCESSION   XP_077504893
VERSION     XP_077504893.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648767.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..595
                     /product="uncharacterized protein LOC144114799"
                     /calculated_mol_wt=63932
     CDS             1..595
                     /gene="LOC144114799"
                     /coded_by="XM_077648767.1:14..1801"
                     /db_xref="GeneID:144114799"
ORIGIN      
        1 maavagtsgl ksfessstta kpavddddde lealrtaalm smkarekhkp nslnseprer
       61 pgayssysry hhsnqrampg kplfswsqht rpnliviqpv plegseaapp snrasiqtaq
      121 pssklllpqd rwcpqqgqds pasassgpsk rrgsgkfshf essseseesd ddllpsgsvs
      181 spsdpshghs sddeqnsacl splrdsldgc lsestsdllv sakwhemspe tgttdcarks
      241 gdvysalhts ghgssdgids giahapsqas gddsesgedv ddctakdllg lvsrqepsve
      301 dglknsavps envqelpvgs pglcgsrsgn dspvghmark spsrkeypqk hearltngvs
      361 ldvqkvhvap dsqredwfap lsreknssre gtpkstrsvd sqdravssvf earrrkfest
      421 vpvkpvggki vllkgqsktd elspchessp lpspraqars rsrstsppfl rsvlsvvkrg
      481 pgellpacae pkkrhrsgrt aevivhsvge essdasdria cssklpvhlr lgdpsmtrkk
      541 kskrkrsshd ssgysakrkh akgrcpdsdv rrtwlgpeqr rrrkcmpsah cdgap
//
LOCUS       XP_077504894             595 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114799 [Amblyomma americanum].
ACCESSION   XP_077504894
VERSION     XP_077504894.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648768.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..595
                     /product="uncharacterized protein LOC144114799"
                     /calculated_mol_wt=63932
     CDS             1..595
                     /gene="LOC144114799"
                     /coded_by="XM_077648768.1:14..1801"
                     /db_xref="GeneID:144114799"
ORIGIN      
        1 maavagtsgl ksfessstta kpavddddde lealrtaalm smkarekhkp nslnseprer
       61 pgayssysry hhsnqrampg kplfswsqht rpnliviqpv plegseaapp snrasiqtaq
      121 pssklllpqd rwcpqqgqds pasassgpsk rrgsgkfshf essseseesd ddllpsgsvs
      181 spsdpshghs sddeqnsacl splrdsldgc lsestsdllv sakwhemspe tgttdcarks
      241 gdvysalhts ghgssdgids giahapsqas gddsesgedv ddctakdllg lvsrqepsve
      301 dglknsavps envqelpvgs pglcgsrsgn dspvghmark spsrkeypqk hearltngvs
      361 ldvqkvhvap dsqredwfap lsreknssre gtpkstrsvd sqdravssvf earrrkfest
      421 vpvkpvggki vllkgqsktd elspchessp lpspraqars rsrstsppfl rsvlsvvkrg
      481 pgellpacae pkkrhrsgrt aevivhsvge essdasdria cssklpvhlr lgdpsmtrkk
      541 kskrkrsshd ssgysakrkh akgrcpdsdv rrtwlgpeqr rrrkcmpsah cdgap
//
LOCUS       XP_077504895             602 aa            linear   INV 25-NOV-2025
DEFINITION  kelch protein [Amblyomma americanum].
ACCESSION   XP_077504895
VERSION     XP_077504895.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648769.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..602
                     /product="kelch protein"
                     /calculated_mol_wt=66416
     Region          45..164
                     /region_name="BTB_POZ_KLHL2-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in Kelch-like
                     proteins, KLHL2 and KLHL3; cd18235"
                     /db_xref="CDD:349544"
     Region          54..588
                     /region_name="PHA03098"
                     /note="kelch-like protein; Provisional"
                     /db_xref="CDD:222983"
     Site            order(89..90,93..94,98,100,107..110,140,143..144,146,151)
                     /site_type="other"
                     /note="cullin binding site [polypeptide binding]"
                     /db_xref="CDD:349544"
     Region          162..275
                     /region_name="BACK_KLHL2_like"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     Kelch-like proteins, KLHL2 and KLHL3; cd18445"
                     /db_xref="CDD:350520"
     Site            order(175..178,180)
                     /site_type="other"
                     /note="cullin binding site [polypeptide binding]"
                     /db_xref="CDD:350520"
     Region          352..395
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          399..443
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          446..491
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          495..539
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          542..587
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     CDS             1..602
                     /gene="kel"
                     /coded_by="XM_077648769.1:220..2028"
                     /db_xref="GeneID:144114800"
ORIGIN      
        1 mnkssfyqam sselasestm lefklqkfdl arsqhslter ppyrnqhhtq kafetmnilr
       61 kqnllcdvtl vagatevsah ktvlascspy fyamftgfte srankitlqg ldgaalaili
      121 dyvysaeiqv teenvqtllp aanllqlsdv qeacceflqa qlhpsnclgi rafadlhgcl
      181 dllshcdtyi eqhfvevten eeflalpanq vahlissdrl svpseeqvfe avmnwvnqdl
      241 anresqlgsl mehvrlplls qeylvqrvee epllkgnlpc kdflieamky hllradqkal
      301 yatprtkprt pigrpkmllv vggqapkair svecldlqre rwlqlaelps rrcraglall
      361 dgrvftvggf ngslrvrtvd iydpvrdqws qapsmearrs tlgvavlnnq iyavggfdgs
      421 tglnsaeryd phaeewtaia smstrrssvg vgvlngllya vggydgasrq clssverydp
      481 keeewslvad msarrsgagv gvldgvlyav gghdgplvrk svecyhpesn swshvpdmal
      541 arrnagvvam dgllyvvggd dgssnlssve vynpktkswn ilstfmtigr syagvtiidr
      601 pl
//
LOCUS       XP_077504896             362 aa            linear   INV 25-NOV-2025
DEFINITION  glyoxylate reductase/hydroxypyruvate reductase-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077504896
VERSION     XP_077504896.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648770.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..362
                     /product="glyoxylate reductase/hydroxypyruvate
                     reductase-like isoform X1"
                     /calculated_mol_wt=38970
     Region          42..354
                     /region_name="GDH"
                     /note="D-glycerate dehydrogenase/hydroxypyruvate reductase
                     (GDH); cd05301"
                     /db_xref="CDD:240626"
     Site            order(48..49,51,92..94,115,143..144,147..148,150..151,154,
                     158,160,168,170,172,176..182,184..188,206..207,210..211,
                     309..310,318,324..330,332..335,337)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:240626"
     Site            order(93,116..118,141,280,328,331)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:240626"
     Site            order(117..118,145,196..200,219..221,224,250..252,
                     256..257,278..280,304..305,328,330..331)
                     /site_type="other"
                     /note="NADP binding site [chemical binding]"
                     /db_xref="CDD:240626"
     CDS             1..362
                     /gene="LOC144114801"
                     /coded_by="XM_077648770.1:114..1202"
                     /db_xref="GeneID:144114801"
ORIGIN      
        1 mwhrcqplin arralltfnt ltltdtvlqr agisamgpar rptvlvtrhd vakealdllt
       61 dkcdvevwne dkpisrsall qkiagkdalf clvtekvdse lldaagsslk vvgtmsvgyd
      121 hidisqctkr rvavgntphv ltdataelgi alllatarrl feahqaigsg ewakqawspt
      181 wmcgseirgs tvgivgmgni gfgilerlka fkaskflyfs rahkpaaeal gaqftrldel
      241 lklsdfviac caltpetkgl fnkeafsmmk statfinisr gqvvdqdaly ealssgkirs
      301 agldvmtpep lpvdhpllkl pncvilphig saatetrtam avltaqnila aldginmpam
      361 vc
//
LOCUS       XP_077504897             323 aa            linear   INV 25-NOV-2025
DEFINITION  glyoxylate reductase/hydroxypyruvate reductase-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077504897
VERSION     XP_077504897.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648771.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..323
                     /product="glyoxylate reductase/hydroxypyruvate
                     reductase-like isoform X2"
                     /calculated_mol_wt=34864
     Region          42..315
                     /region_name="GDH"
                     /note="D-glycerate dehydrogenase/hydroxypyruvate reductase
                     (GDH); cd05301"
                     /db_xref="CDD:240626"
     Site            order(48..49,51,92..94,115,131,133,137..143,145..149,
                     167..168,171..172,270..271,279,285..291,293..296,298)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:240626"
     Site            order(93,116..118,241,289,292)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:240626"
     Site            order(117..118,157..161,180..182,185,211..213,217..218,
                     239..241,265..266,289,291..292)
                     /site_type="other"
                     /note="NADP binding site [chemical binding]"
                     /db_xref="CDD:240626"
     CDS             1..323
                     /gene="LOC144114801"
                     /coded_by="XM_077648771.1:114..1085"
                     /db_xref="GeneID:144114801"
ORIGIN      
        1 mwhrcqplin arralltfnt ltltdtvlqr agisamgpar rptvlvtrhd vakealdllt
       61 dkcdvevwne dkpisrsall qkiagkdalf clvtekvdse lldaagsslk vvgtmsvgyd
      121 hidisqctks gewakqawsp twmcgseirg stvgivgmgn igfgilerlk afkaskflyf
      181 srahkpaaea lgaqftrlde llklsdfvia ccaltpetkg lfnkeafsmm kstatfinis
      241 rgqvvdqdal yealssgkir sagldvmtpe plpvdhpllk lpncvilphi gsaatetrta
      301 mavltaqnil aaldginmpa mvc
//
LOCUS       XP_077504898             604 aa            linear   INV 25-NOV-2025
DEFINITION  high-affinity choline transporter 1-like [Amblyomma americanum].
ACCESSION   XP_077504898
VERSION     XP_077504898.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648772.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..604
                     /product="high-affinity choline transporter 1-like"
                     /calculated_mol_wt=64939
     Region          8..484
                     /region_name="SLC5sbd_CHT"
                     /note="Na(+)- and Cl(-)-dependent choline cotransporter
                     CHT and related proteins; solute-binding domain; cd11474"
                     /db_xref="CDD:271368"
     Site            order(60,63,335,338..339)
                     /site_type="other"
                     /note="Na binding site [ion binding]"
                     /db_xref="CDD:271368"
     CDS             1..604
                     /gene="LOC144114803"
                     /coded_by="XM_077648772.1:305..2119"
                     /db_xref="GeneID:144114803"
ORIGIN      
        1 mainiagvvs vvifyiiila vgiwagrksk ktgndpgdad evmlagrnig vfvgiftmta
       61 twvgggying taeavysngi iwcqapvgya mslvvggyff ankmraegyv tmldpfqelf
      121 ggrmggllfi palcgevfws aailaalgat vgviididtn tsiiasacia lfytffggly
      181 svaytdviql fcifiglwlc ipfcminesv gtlsyptndw vgslepryvg qyidfgllli
      241 lggipwqvyf qrvlscktaf kaqllsyvaa fgciimaipa mimgavakat rwnetaftgp
      301 lpldkehtsl vlpmvlqflt pgfvsfvglg avsaavmsss dssilsaasm farnvyklif
      361 rqnaserevi wvmrvailfv gamatamalt vksiyglwyl ssdlvyvilf pqlvcvvyik
      421 dhcntygslg ayiiglllrg lggedilglp pvirypfyne tdgqlfpfrt lamltslasi
      481 vsisslmrwl fesgtiparf dvfhcvvnip edimkvqeph egemtvlnag glvktyqtem
      541 ngrvnpalnl hpeedvegfq vksaaavgad plspfgggpl aanaappplg ergdalssed
      601 ttkl
//
LOCUS       XP_077504899             604 aa            linear   INV 25-NOV-2025
DEFINITION  high-affinity choline transporter 1-like [Amblyomma americanum].
ACCESSION   XP_077504899
VERSION     XP_077504899.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648773.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..604
                     /product="high-affinity choline transporter 1-like"
                     /calculated_mol_wt=64939
     Region          8..484
                     /region_name="SLC5sbd_CHT"
                     /note="Na(+)- and Cl(-)-dependent choline cotransporter
                     CHT and related proteins; solute-binding domain; cd11474"
                     /db_xref="CDD:271368"
     Site            order(60,63,335,338..339)
                     /site_type="other"
                     /note="Na binding site [ion binding]"
                     /db_xref="CDD:271368"
     CDS             1..604
                     /gene="LOC144114803"
                     /coded_by="XM_077648773.1:360..2174"
                     /db_xref="GeneID:144114803"
ORIGIN      
        1 mainiagvvs vvifyiiila vgiwagrksk ktgndpgdad evmlagrnig vfvgiftmta
       61 twvgggying taeavysngi iwcqapvgya mslvvggyff ankmraegyv tmldpfqelf
      121 ggrmggllfi palcgevfws aailaalgat vgviididtn tsiiasacia lfytffggly
      181 svaytdviql fcifiglwlc ipfcminesv gtlsyptndw vgslepryvg qyidfgllli
      241 lggipwqvyf qrvlscktaf kaqllsyvaa fgciimaipa mimgavakat rwnetaftgp
      301 lpldkehtsl vlpmvlqflt pgfvsfvglg avsaavmsss dssilsaasm farnvyklif
      361 rqnaserevi wvmrvailfv gamatamalt vksiyglwyl ssdlvyvilf pqlvcvvyik
      421 dhcntygslg ayiiglllrg lggedilglp pvirypfyne tdgqlfpfrt lamltslasi
      481 vsisslmrwl fesgtiparf dvfhcvvnip edimkvqeph egemtvlnag glvktyqtem
      541 ngrvnpalnl hpeedvegfq vksaaavgad plspfgggpl aanaappplg ergdalssed
      601 ttkl
//
LOCUS       XP_077504900             203 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114804 [Amblyomma americanum].
ACCESSION   XP_077504900
VERSION     XP_077504900.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648774.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..203
                     /product="uncharacterized protein LOC144114804"
                     /calculated_mol_wt=23538
     CDS             1..203
                     /gene="LOC144114804"
                     /coded_by="XM_077648774.1:147..758"
                     /db_xref="GeneID:144114804"
ORIGIN      
        1 mledaskacg dtleaceqek pekvdaaepa geppdqelel pmpssptslk rhlrkaygrr
       61 pmelvsryfr slhdlatfan netflsrcrd lqmvppayrv ecrgikntqh virildkcsy
      121 rlmladleyn kvrkvqvsrl leiqheklkm imteedlhyi lmmskekyen vfeatrdeqr
      181 gafaqllrey eirleedags dnv
//
LOCUS       XP_077504902             791 aa            linear   INV 25-NOV-2025
DEFINITION  membrane metallo-endopeptidase-like 1 [Amblyomma americanum].
ACCESSION   XP_077504902
VERSION     XP_077504902.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648776.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..791
                     /product="membrane metallo-endopeptidase-like 1"
                     /calculated_mol_wt=88685
     Region          168..780
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..791
                     /gene="LOC144114805"
                     /coded_by="XM_077648776.1:117..2492"
                     /db_xref="GeneID:144114805"
ORIGIN      
        1 malrpqvrrd ssvtpiihsf vlapeasffs pthdessqek qlskppfdpl ihsyrlaitd
       61 sqtrlrrhss lkttrrqvpr svsfdiaapp sisasrrrrg gekyitvprr apgersteyr
      121 vfmvsattlt laftlvqplv lfhclapcqe prcynvamiv hesitpsvap cqdfftyavg
      181 naganryftr ieltarraal hwistieeas sgqsavqkaa allrgcmklt mqknedldai
      241 rnvlsaggle fpkmapttka dvirsivefn lnaglapifr lttgrsiyee egympyvapa
      301 yhvlsfadhl qqlgspenfq vyvrrcaevv gepgmsyaal iravssvnql faelsvtkpi
      361 hvelklsssy pktdfwrsls avlrdkfesl gimkstflvv ddsyfqflvs kvfgtlegve
      421 itayiglylv wylsrvasyt laysspvpny tnelndmwlr cfadvhtvmp faaeqlylrd
      481 vinktdvqei esivhtvtqa tkaflsafpr egipsearli egignlrifp ytfaalhdad
      541 qldslydyvp dqedvdymyn ylrvsrataq yvlrllhnss aaptgpfvpp feagvpfmas
      601 vynmihfrpd tmvppafsfg qslsldyagi gynvaeqvgq lflsgpsvln esgcyervws
      661 adffnalrsk yqclrrlfld selnatelps dairasvtae ilykaytgir hspaladrfr
      721 reaeefglap dqlffvglcm nwrqrkhepr gmaeivcrfs lpsvpgfrdv fscprtaaln
      781 gtfsvqeqcy c
//
LOCUS       XP_077504903            1776 aa            linear   INV 25-NOV-2025
DEFINITION  cadherin 88C [Amblyomma americanum].
ACCESSION   XP_077504903
VERSION     XP_077504903.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648777.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1776
                     /product="cadherin 88C"
                     /calculated_mol_wt=196830
     Region          21..120
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(26..27,82,84,117,119..120)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          131..231
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(136..137,188,190,228,230..231)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          241..349
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(247..248,311,313,345,347..348)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          357..459
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(364..365,422,424,455,457..458)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          467..559
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          585..684
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(588..589,643,649,682,684)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          695..806
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(700..701,767,769,802,804..805)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          815..910
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(821..822,873,875,906,908..909)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          920..1020
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(925..926,978,980,1017,1019..1020)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1032..1130
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1036..1037,1095,1126,1128..1129)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1142..1239
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1147..1148,1202,1204,1236,1238..1239)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1250..1359
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1255..1256,1312,1314,1355,1357..1358)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1379..1468
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     CDS             1..1776
                     /gene="Cad88C"
                     /coded_by="XM_077648777.1:820..6150"
                     /db_xref="GeneID:144114806"
ORIGIN      
        1 mliivddvnd nepvfkqyrs titvpedasp hvidtveaid rdqgpfgqvi yqlqntdset
       61 tstfsvqqdk gkgiisligp ldyerkfiye lrvlaidran agprhtatav vlvkvddved
      121 qppiftqvpp vtrisedlpl nsvvltvkam dgdrgvnnai hysiisggng lfaidretgv
      181 vsvkgrldre atenrngafi llvqaqevss nvkpapstet evtvlltdvn deepafrsrr
      241 yiaeinenaq invpvnfvge svpevfdhdq gsngtfqlfl qedngvfdvt paigvneasf
      301 mirvknpdyl dyervkvmnf kivaremvss qpktstadvt vhikdandnf pvfredvyra
      361 tipenaasgt vvavvraeda dseaygtagi rytsirgqia dklrldpesg kvvvatsahg
      421 ldretapehf ltveardeng rgnrntvqlh lvledindna ptfvlpsyea rirenaldfr
      481 tplvvmavda dqaggpnsqv ryqivhgdpd nnftidevkg rikprlpldf eriaqprgdv
      541 rtfnllvray dmgrpslssn vtvvvyvqdv ndhapefptd fvsksipedt qegtaivrvl
      601 aedrdhspsn skvvyriqng aqdkfvidal tgvisvapga nmdpdrtfpk svlyslqila
      661 ldgglgeqqk ssmvtvnisi idvnnkaprf eepgtvnlve nsvpghtvai vratdqddrp
      721 mlryslchga ssearnedgg lvsefdagqs fdinnvdgkv mvksnidrev tesiklcvkv
      781 edmaaatpgq tatatltinv vdvndnrpsf kksyyrqtvt ensmigapiv taraidadkn
      841 rtvfyslegk meylaligid rktgevtvad kidrethswi nvtlkatdsg fpplsgatel
      901 qiqvldendn npifrpgpse favsedaplg ssvaavqaad advgeygrvt yaldaagtdg
      961 kfkidrdtgv itvampldre etasyslivq awdnyeagfg tdesrrtfkq vsirvldvnd
     1021 espvfvrqth caevtefhrs hetvllasat daddpttpna rivftiqsgy eadlfevesl
     1081 pdntarlstr fslrgrvgnv tlalraedqg lpprssvqtf sicvadvndh spvfvrppqn
     1141 htiripenat lgtvvvevga edndfganae vryrlkdlpn ghwktfrlde rtgvitlrqt
     1201 ldretqkvye lrveaydlgq ptslssdldl tvfvtdvndy apeftedvhq ltftenlkpg
     1261 vetyklisti drdddlsilk pipcyyivgg nekgrfvldi fthqlsatep ldreeqanyt
     1321 lvvrasddcf heprpvsrfd pkdntllqve isvldvndnp prfvsrvftg gvtmeadfgv
     1381 vfmtvkavdr deginsvvsy yiggeirrtl seglesltgp pflinhrtgd lslnfypqkg
     1441 mkgyfdfevi andtegfydt asvfiyllre dqrvrfvlrl tpeefrekme efrevlanit
     1501 gaivnideyk vhenedgsvd rkksdlylhf vnrddnsime vsnvltlidk niefldklfk
     1561 efnvlysepa dalsdaeree dqmrtwligl svflsvmlal vislclaqrs ryerqlkaat
     1621 atafgsrdsa lnrmdvpntn qhsvegsnpi wmhsydnewy kedeetraad rnsldenava
     1681 epsgstssst dthrqtqdre fdsaqlcsev yvlsngtvqn isipvggstd llcpevaern
     1741 nlnsrnnkng gcpyasvflt kmgtpnragk levsel
//
LOCUS       XP_077504904             214 aa            linear   INV 25-NOV-2025
DEFINITION  eukaryotic translation initiation factor 4E-1A-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077504904
VERSION     XP_077504904.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648778.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..214
                     /product="eukaryotic translation initiation factor
                     4E-1A-like isoform X1"
                     /calculated_mol_wt=24500
     Region          35..194
                     /region_name="IF4E"
                     /note="Eukaryotic initiation factor 4E; pfam01652"
                     /db_xref="CDD:460281"
     CDS             1..214
                     /gene="LOC144114808"
                     /coded_by="XM_077648778.1:132..776"
                     /db_xref="GeneID:144114808"
ORIGIN      
        1 maaedkyses dsaepgaseq apavdaslel pvkhplqhrw slwfykndkn rsweenllei
       61 tsfdtvedfw alynhievas klplgcdysl fkygikpmwe dcrnkqggrw lfnlnksqrt
      121 tdldnywlei llcligegfd efsddvcgav vqirakldki avwtadvrra dgnikigktl
      181 kerlnmhprn ivpyqahadt qskqgstara ryqv
//
LOCUS       XP_077504905             214 aa            linear   INV 25-NOV-2025
DEFINITION  eukaryotic translation initiation factor 4E-1A-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077504905
VERSION     XP_077504905.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648779.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..214
                     /product="eukaryotic translation initiation factor
                     4E-1A-like isoform X2"
                     /calculated_mol_wt=24475
     Region          35..194
                     /region_name="IF4E"
                     /note="Eukaryotic initiation factor 4E; pfam01652"
                     /db_xref="CDD:460281"
     CDS             1..214
                     /gene="LOC144114808"
                     /coded_by="XM_077648779.1:6170..6814"
                     /db_xref="GeneID:144114808"
ORIGIN      
        1 mlgpcswpqs dsaepgaseq apavdaslel pvkhplqhrw slwfykndkn rsweenllei
       61 tsfdtvedfw alynhievas klplgcdysl fkygikpmwe dcrnkqggrw lfnlnksqrt
      121 tdldnywlei llcligegfd efsddvcgav vqirakldki avwtadvrra dgnikigktl
      181 kerlnmhprn ivpyqahadt qskqgstara ryqv
//
LOCUS       XP_077504906             214 aa            linear   INV 25-NOV-2025
DEFINITION  eukaryotic translation initiation factor 4E-1A-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077504906
VERSION     XP_077504906.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648780.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..214
                     /product="eukaryotic translation initiation factor
                     4E-1A-like isoform X2"
                     /calculated_mol_wt=24475
     Region          35..194
                     /region_name="IF4E"
                     /note="Eukaryotic initiation factor 4E; pfam01652"
                     /db_xref="CDD:460281"
     CDS             1..214
                     /gene="LOC144114808"
                     /coded_by="XM_077648780.1:3696..4340"
                     /db_xref="GeneID:144114808"
ORIGIN      
        1 mlgpcswpqs dsaepgaseq apavdaslel pvkhplqhrw slwfykndkn rsweenllei
       61 tsfdtvedfw alynhievas klplgcdysl fkygikpmwe dcrnkqggrw lfnlnksqrt
      121 tdldnywlei llcligegfd efsddvcgav vqirakldki avwtadvrra dgnikigktl
      181 kerlnmhprn ivpyqahadt qskqgstara ryqv
//
LOCUS       XP_077504907             130 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114809 [Amblyomma americanum].
ACCESSION   XP_077504907
VERSION     XP_077504907.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648781.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..130
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..130
                     /product="uncharacterized protein LOC144114809"
                     /calculated_mol_wt=14582
     CDS             1..130
                     /gene="LOC144114809"
                     /coded_by="XM_077648781.1:87..479"
                     /db_xref="GeneID:144114809"
ORIGIN      
        1 maspgasslv kfnirwgnrs nqssimntse yidtlkkrlr avimknkila ralaaskkei
       61 selkksngrq qkvfnlglpq vktwlkmarq hshtmyqyqs raltlldeim npgvdftlar
      121 vsdadddadi
//
LOCUS       XP_077504908             945 aa            linear   INV 25-NOV-2025
DEFINITION  phosphodiesterase 11 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504908
VERSION     XP_077504908.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648782.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..945
                     /product="phosphodiesterase 11 isoform X1"
                     /calculated_mol_wt=106303
     Region          237..400
                     /region_name="GAF"
                     /note="Domain present in phytochromes and cGMP-specific
                     phosphodiesterases; smart00065"
                     /db_xref="CDD:214500"
     Region          422..581
                     /region_name="GAF"
                     /note="Domain present in phytochromes and cGMP-specific
                     phosphodiesterases; smart00065"
                     /db_xref="CDD:214500"
     Region          677..911
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..945
                     /gene="Pde11"
                     /coded_by="XM_077648782.1:133..2970"
                     /db_xref="GeneID:144114811"
ORIGIN      
        1 mlpgpasgkr srlaagetsg sggralwrpl wqpgwhprga ppplllpsss amkaseggge
       61 qleearveaw ldehpafvgd yfmrkasrkr rrcypqvvds wllahavpqs vvqasdqaag
      121 scsssmlaaa hslssssssl nggssgagan isppmrkisa cefdrgsplr pivqtsfdgt
      181 ptfltlpaat esqsgvtgap ggaglvrpkr rsrqelqaln erelifelvk dicneldirl
      241 lchkilqnvt vitdadrcsl flvhgdrdsp agrclvsqlf dvscrstldq vgyeevrvpw
      301 gsgivgyvaq skeavnipdc yndarfnksi dlrtgyrtrc mlcmpifdrd gevigvaqvi
      361 nkkgknehdv fnsndekvfs eylqfcglvl rnaqlyerse lenkrnqvll dlarmvfeeq
      421 stielvvyri mthmqsllec ercqvllvdh etktafhrvf dleakdalre dvatctspye
      481 grfpintgit gsvvatgqil nipdaykddr fdpkvdegss frhqsilcip iynanrlvlg
      541 vaqlinklng epfnkndeql leafaifcgl giqntqmyek vvkamakqrv tlevlsyhas
      601 apleealkln ecvlpsmqsl hlqdykfndf sldddgmlka clrifleldf lnrfhieylv
      661 lcrwllsvkk nyrkvsyhnw rhafnvgqmm fciltntklh evlgdletfg livacfchdl
      721 dhrgtnnsfq iksssplaql yststmehhh fdqcvmilns qgnqilshvs peeyttiiri
      781 veeailatdl avyfrkrgqf fklvdnkeyq wtnadhrell rnmlmtacdi gaitkpwdvq
      841 rvvadlvtme ffqqgdmekq elnlqpidmm nrekkdqlps mqlgfidsic lplyrsfsal
      901 ndrlyplleg velnrmewkr ladahiasin gdrsdsddtd arkkt
//
LOCUS       XP_077504909             938 aa            linear   INV 25-NOV-2025
DEFINITION  phosphodiesterase 11 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504909
VERSION     XP_077504909.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648783.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..938
                     /product="phosphodiesterase 11 isoform X2"
                     /calculated_mol_wt=105343
     Region          230..393
                     /region_name="GAF"
                     /note="Domain present in phytochromes and cGMP-specific
                     phosphodiesterases; smart00065"
                     /db_xref="CDD:214500"
     Region          415..574
                     /region_name="GAF"
                     /note="Domain present in phytochromes and cGMP-specific
                     phosphodiesterases; smart00065"
                     /db_xref="CDD:214500"
     Region          670..904
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..938
                     /gene="Pde11"
                     /coded_by="XM_077648783.1:379..3195"
                     /db_xref="GeneID:144114811"
ORIGIN      
        1 mlpgpasgkr srlaagetsg sggralwrpl wqpgwhprga ppplllpsss amkaseggge
       61 qleearveaw ldehpafvgd yfmrkasrkv vdswllahav pqsvvqasdq aagscsssml
      121 aaahslssss sslnggssga ganisppmrk isacefdrgs plrpivqtsf dgtptfltlp
      181 aatesqsgvt gapggaglvr pkrrsrqelq alnerelife lvkdicneld irllchkilq
      241 nvtvitdadr cslflvhgdr dspagrclvs qlfdvscrst ldqvgyeevr vpwgsgivgy
      301 vaqskeavni pdcyndarfn ksidlrtgyr trcmlcmpif drdgevigva qvinkkgkne
      361 hdvfnsndek vfseylqfcg lvlrnaqlye rselenkrnq vlldlarmvf eeqstielvv
      421 yrimthmqsl lecercqvll vdhetktafh rvfdleakda lredvatcts pyegrfpint
      481 gitgsvvatg qilnipdayk ddrfdpkvde gssfrhqsil cipiynanrl vlgvaqlink
      541 lngepfnknd eqlleafaif cglgiqntqm yekvvkamak qrvtlevlsy hasapleeal
      601 klnecvlpsm qslhlqdykf ndfsldddgm lkaclrifle ldflnrfhie ylvlcrwlls
      661 vkknyrkvsy hnwrhafnvg qmmfciltnt klhevlgdle tfglivacfc hdldhrgtnn
      721 sfqikssspl aqlyststme hhhfdqcvmi lnsqgnqils hvspeeytti iriveeaila
      781 tdlavyfrkr gqffklvdnk eyqwtnadhr ellrnmlmta cdigaitkpw dvqrvvadlv
      841 tmeffqqgdm ekqelnlqpi dmmnrekkdq lpsmqlgfid siclplyrsf salndrlypl
      901 legvelnrme wkrladahia singdrsdsd dtdarkkt
//
LOCUS       XP_077504910             894 aa            linear   INV 25-NOV-2025
DEFINITION  phosphodiesterase 11 isoform X3 [Amblyomma americanum].
ACCESSION   XP_077504910
VERSION     XP_077504910.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648784.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..894
                     /product="phosphodiesterase 11 isoform X3"
                     /calculated_mol_wt=101050
     Region          186..349
                     /region_name="GAF"
                     /note="Domain present in phytochromes and cGMP-specific
                     phosphodiesterases; smart00065"
                     /db_xref="CDD:214500"
     Region          371..530
                     /region_name="GAF"
                     /note="Domain present in phytochromes and cGMP-specific
                     phosphodiesterases; smart00065"
                     /db_xref="CDD:214500"
     Region          626..860
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..894
                     /gene="Pde11"
                     /coded_by="XM_077648784.1:561..3245"
                     /db_xref="GeneID:144114811"
ORIGIN      
        1 mkasegggeq leearveawl dehpafvgdy fmrkasrkrr rcypqvvdsw llahavpqsv
       61 vqasdqaags csssmlaaah slssssssln ggssgagani sppmrkisac efdrgsplrp
      121 ivqtsfdgtp tfltlpaate sqsgvtgapg gaglvrpkrr srqelqalne relifelvkd
      181 icneldirll chkilqnvtv itdadrcslf lvhgdrdspa grclvsqlfd vscrstldqv
      241 gyeevrvpwg sgivgyvaqs keavnipdcy ndarfnksid lrtgyrtrcm lcmpifdrdg
      301 evigvaqvin kkgknehdvf nsndekvfse ylqfcglvlr naqlyersel enkrnqvlld
      361 larmvfeeqs tielvvyrim thmqsllece rcqvllvdhe tktafhrvfd leakdalred
      421 vatctspyeg rfpintgitg svvatgqiln ipdaykddrf dpkvdegssf rhqsilcipi
      481 ynanrlvlgv aqlinklnge pfnkndeqll eafaifcglg iqntqmyekv vkamakqrvt
      541 levlsyhasa pleealklne cvlpsmqslh lqdykfndfs ldddgmlkac lrifleldfl
      601 nrfhieylvl crwllsvkkn yrkvsyhnwr hafnvgqmmf ciltntklhe vlgdletfgl
      661 ivacfchdld hrgtnnsfqi ksssplaqly ststmehhhf dqcvmilnsq gnqilshvsp
      721 eeyttiiriv eeailatdla vyfrkrgqff klvdnkeyqw tnadhrellr nmlmtacdig
      781 aitkpwdvqr vvadlvtmef fqqgdmekqe lnlqpidmmn rekkdqlpsm qlgfidsicl
      841 plyrsfsaln drlypllegv elnrmewkrl adahiasing drsdsddtda rkkt
//
LOCUS       XP_077504911             887 aa            linear   INV 25-NOV-2025
DEFINITION  phosphodiesterase 11 isoform X4 [Amblyomma americanum].
ACCESSION   XP_077504911
VERSION     XP_077504911.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648785.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..887
                     /product="phosphodiesterase 11 isoform X4"
                     /calculated_mol_wt=100090
     Region          179..342
                     /region_name="GAF"
                     /note="Domain present in phytochromes and cGMP-specific
                     phosphodiesterases; smart00065"
                     /db_xref="CDD:214500"
     Region          364..523
                     /region_name="GAF"
                     /note="Domain present in phytochromes and cGMP-specific
                     phosphodiesterases; smart00065"
                     /db_xref="CDD:214500"
     Region          619..853
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..887
                     /gene="Pde11"
                     /coded_by="XM_077648785.1:1167..3830"
                     /db_xref="GeneID:144114811"
ORIGIN      
        1 mkasegggeq leearveawl dehpafvgdy fmrkasrkvv dswllahavp qsvvqasdqa
       61 agscsssmla aahslsssss slnggssgag anisppmrki sacefdrgsp lrpivqtsfd
      121 gtptfltlpa atesqsgvtg apggaglvrp krrsrqelqa lnerelifel vkdicneldi
      181 rllchkilqn vtvitdadrc slflvhgdrd spagrclvsq lfdvscrstl dqvgyeevrv
      241 pwgsgivgyv aqskeavnip dcyndarfnk sidlrtgyrt rcmlcmpifd rdgevigvaq
      301 vinkkgkneh dvfnsndekv fseylqfcgl vlrnaqlyer selenkrnqv lldlarmvfe
      361 eqstielvvy rimthmqsll ecercqvllv dhetktafhr vfdleakdal redvatctsp
      421 yegrfpintg itgsvvatgq ilnipdaykd drfdpkvdeg ssfrhqsilc ipiynanrlv
      481 lgvaqlinkl ngepfnknde qlleafaifc glgiqntqmy ekvvkamakq rvtlevlsyh
      541 asapleealk lnecvlpsmq slhlqdykfn dfsldddgml kaclriflel dflnrfhiey
      601 lvlcrwllsv kknyrkvsyh nwrhafnvgq mmfciltntk lhevlgdlet fglivacfch
      661 dldhrgtnns fqikssspla qlyststmeh hhfdqcvmil nsqgnqilsh vspeeyttii
      721 riveeailat dlavyfrkrg qffklvdnke yqwtnadhre llrnmlmtac digaitkpwd
      781 vqrvvadlvt meffqqgdme kqelnlqpid mmnrekkdql psmqlgfids iclplyrsfs
      841 alndrlypll egvelnrmew krladahias ingdrsdsdd tdarkkt
//
LOCUS       XP_077504913             162 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114812 [Amblyomma americanum].
ACCESSION   XP_077504913
VERSION     XP_077504913.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648787.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..162
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..162
                     /product="uncharacterized protein LOC144114812"
                     /calculated_mol_wt=17296
     CDS             1..162
                     /gene="LOC144114812"
                     /coded_by="XM_077648787.1:120..608"
                     /db_xref="GeneID:144114812"
ORIGIN      
        1 mqvtgfssgp csgarhaspv fqtlktaasv frmllskywt qtlfasrrgl sdvdlsaedg
       61 gpssageqes gddpaqtatg katprhptlv prgeappala scvhgrpasl npfrdaavch
      121 acfrayhpqy ysrlrsrlsp lerrmeresh glraggqyaa lp
//
LOCUS       XP_077504914             243 aa            linear   INV 25-NOV-2025
DEFINITION  protein spitz-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504914
VERSION     XP_077504914.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648788.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..243
                     /product="protein spitz-like isoform X1"
                     /calculated_mol_wt=27064
     Region          <112..143
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     CDS             1..243
                     /gene="LOC144114813"
                     /coded_by="XM_077648788.1:251..982"
                     /db_xref="GeneID:144114813"
ORIGIN      
        1 mrvtpitvrg gvlaaarndq lerskcagss sspspassaw wpkgllqvds rrlatcppsw
       61 llflvfaaal lriadgcssr stpkprppqs tvrpnitfqt ytcpetyakw yclngatcfs
      121 ikigesilyn cecadgymgq rcefkdldgt ylptrqrvli etasiaggvt mavilvfivc
      181 itvylyyqrr kdktyfdvpd epklffcdrk ktpnqlphpv lrqsdlergr gvhmcdnsrr
      241 sss
//
LOCUS       XP_077504915             227 aa            linear   INV 25-NOV-2025
DEFINITION  protein spitz-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504915
VERSION     XP_077504915.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648789.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..227
                     /product="protein spitz-like isoform X2"
                     /calculated_mol_wt=25365
     Region          <96..127
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     CDS             1..227
                     /gene="LOC144114813"
                     /coded_by="XM_077648789.1:601..1284"
                     /db_xref="GeneID:144114813"
ORIGIN      
        1 mvleptrqig agsssspspa ssawwpkgll qvdsrrlatc ppswllflvf aaallriadg
       61 cssrstpkpr ppqstvrpni tfqtytcpet yakwyclnga tcfsikiges ilyncecadg
      121 ymgqrcefkd ldgtylptrq rvlietasia ggvtmavilv fivcitvyly yqrrkdktyf
      181 dvpdepklff cdrkktpnql phpvlrqsdl ergrgvhmcd nsrrsss
//
LOCUS       XP_077504916             222 aa            linear   INV 25-NOV-2025
DEFINITION  protein spitz-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077504916
VERSION     XP_077504916.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648790.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..222
                     /product="protein spitz-like isoform X3"
                     /calculated_mol_wt=24727
     Region          <91..122
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     CDS             1..222
                     /gene="LOC144114813"
                     /coded_by="XM_077648790.1:278..946"
                     /db_xref="GeneID:144114813"
ORIGIN      
        1 malngagsss spspassaww pkgllqvdsr rlatcppswl lflvfaaall riadgcssrs
       61 tpkprppqst vrpnitfqty tcpetyakwy clngatcfsi kigesilync ecadgymgqr
      121 cefkdldgty lptrqrvlie tasiaggvtm avilvfivci tvylyyqrrk dktyfdvpde
      181 pklffcdrkk tpnqlphpvl rqsdlergrg vhmcdnsrrs ss
//
LOCUS       XP_077504917             222 aa            linear   INV 25-NOV-2025
DEFINITION  protein spitz-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077504917
VERSION     XP_077504917.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648791.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..222
                     /product="protein spitz-like isoform X3"
                     /calculated_mol_wt=24727
     Region          <91..122
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     CDS             1..222
                     /gene="LOC144114813"
                     /coded_by="XM_077648791.1:264..932"
                     /db_xref="GeneID:144114813"
ORIGIN      
        1 malngagsss spspassaww pkgllqvdsr rlatcppswl lflvfaaall riadgcssrs
       61 tpkprppqst vrpnitfqty tcpetyakwy clngatcfsi kigesilync ecadgymgqr
      121 cefkdldgty lptrqrvlie tasiaggvtm avilvfivci tvylyyqrrk dktyfdvpde
      181 pklffcdrkk tpnqlphpvl rqsdlergrg vhmcdnsrrs ss
//
LOCUS       XP_077504918             579 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114815 [Amblyomma americanum].
ACCESSION   XP_077504918
VERSION     XP_077504918.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648792.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..579
                     /product="uncharacterized protein LOC144114815"
                     /calculated_mol_wt=65124
     CDS             1..579
                     /gene="LOC144114815"
                     /coded_by="XM_077648792.1:243..1982"
                     /db_xref="GeneID:144114815"
ORIGIN      
        1 msdnenasic flwtaydpcv knavfeyvke kagskklyil rtetladdls sctvfgflvd
       61 alrkrnafvn gsttvlsvpe hfqaihlcaa rvklresakf dttlpevana dfvyqmrlsk
      121 savqfeqsep ecvqeyvefl sqtntvngwq ilrkfrdale ddstfaaqfa nsvflvygpi
      181 teefgkecvd llsqgrkirr ivteapldms anevyniedv svssevaevp kpdrsavekw
      241 dsntvgymvc itnafirvll nssdelalat amaspivelp hdgftqlkll slnkktpmcq
      301 tailhvkrak lggesyampe drplkryvck leefvkllsd lqnkievvpt adaaaviirt
      361 lvlrikaakr hglvkervah flneltrlar rfqdeestgn vddakdtvqg satlnilqrl
      421 sdflstrris clraeliyny gnhgtplnip emmkyfrtpv sededdddyd vplherlakn
      481 cqshsekafl fwlhaeaekk nkrksrrskq qleqqlehel yippkvyavt deaplaelks
      541 agkkaakrsi ladisgtkkk asnnsstdqa ritsffska
//
LOCUS       XP_077504919             413 aa            linear   INV 25-NOV-2025
DEFINITION  leucine-rich repeat protein 1-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504919
VERSION     XP_077504919.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648793.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..413
                     /product="leucine-rich repeat protein 1-like isoform X1"
                     /calculated_mol_wt=46070
     Region          <153..354
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          153..175
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          176..195
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          196..222
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          224..246
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          247..269
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          270..291
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..413
                     /gene="LOC144114816"
                     /coded_by="XM_077648793.1:211..1452"
                     /db_xref="GeneID:144114816"
ORIGIN      
        1 mrldcetqif yrmlqylnhc ersmkatftt ltlyrkpekk kskpvtflll ctaknpagtk
       61 yrvdsniegl ftkfvdegka ririrepkhd viiskaeatk lrqflcllrr vqrgdildqs
      121 vfsdppskit rtrevlvvks rhqypsqgfp ralkeltieg cslvqlssdv lalanlcvln
      181 lkanqiqklp tqlknlpikc ltvsencive lqpelfegrl rsclqsldvs nnqltvlpet
      241 lcsarklafl aarynrlqkl pdglgylsdl welditsnal tflpasmlkl klkcvsiwsn
      301 pltatagqpm ehttasrqvp wplielaara vvaagslelt aedvprsllq llrtatpcyg
      361 cqqpvfpata asvilrvnph ktvapggelm wapthdnsvp vrahicstkc lpq
//
LOCUS       XP_077504920             412 aa            linear   INV 25-NOV-2025
DEFINITION  leucine-rich repeat protein 1-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504920
VERSION     XP_077504920.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648794.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..412
                     /product="leucine-rich repeat protein 1-like isoform X2"
                     /calculated_mol_wt=45983
     Region          <153..>303
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          153..175
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          176..195
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          196..222
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          224..246
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          247..269
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          270..291
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..412
                     /gene="LOC144114816"
                     /coded_by="XM_077648794.1:211..1449"
                     /db_xref="GeneID:144114816"
ORIGIN      
        1 mrldcetqif yrmlqylnhc ersmkatftt ltlyrkpekk kskpvtflll ctaknpagtk
       61 yrvdsniegl ftkfvdegka ririrepkhd viiskaeatk lrqflcllrr vqrgdildqs
      121 vfsdppskit rtrevlvvks rhqypsqgfp ralkeltieg cslvqlssdv lalanlcvln
      181 lkanqiqklp tqlknlpikc ltvsencive lqpelfegrl rsclqsldvs nnqltvlpet
      241 lcsarklafl aarynrlqkl pdglgylsdl welditsnal tflpasmlkl klkcvsiwsn
      301 pltatagqpm ehttasrqvp wplielaara vvaaglelta edvprsllql lrtatpcygc
      361 qqpvfpataa svilrvnphk tvapggelmw apthdnsvpv rahicstkcl pq
//
LOCUS       XP_077504921             493 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114817 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504921
VERSION     XP_077504921.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648795.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..493
                     /product="uncharacterized protein LOC144114817 isoform X1"
                     /calculated_mol_wt=55319
     Region          237..324
                     /region_name="BTB_POZ_ZBTB_KLHL-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in zinc finger and
                     BTB domain-containing (ZBTB) proteins, Kelch-like (KLHL)
                     proteins, and similar proteins; cd18186"
                     /db_xref="CDD:349497"
     Region          365..445
                     /region_name="BACK"
                     /note="BTB And C-terminal Kelch; smart00875"
                     /db_xref="CDD:197943"
     CDS             1..493
                     /gene="LOC144114817"
                     /coded_by="XM_077648795.1:74..1555"
                     /db_xref="GeneID:144114817"
ORIGIN      
        1 mrhrqamcih nvlpdqsaaq lhrngsklkl amqndlgqra dscletcrni atcatrpwgq
       61 ppdfelakrf valwprstig gqaagdhhhl rgkgevvlkl reapaqkytq pvtavsrppk
      121 pariklwvgq rqsavvslsp aatgqamvll kmyrdgfiyy hvseaatapi ntqctsfklk
      181 elfitfpdil teykvccrcy krtncwhwts straivstqt etvpefslwr flgireladv
      241 eivvhwehap eqhasfkahr milalqndvf ramffghfgs gdrvtitdlp pdgvegllry
      301 fysgqhqves vhqatcirsa avkymvpela arcvayvery mepddvcpfl dyiltmggdg
      361 vdgpakavlr nnglfvlask tfesclhctv nyildnvhna pemsvlqavh awgrrqcrer
      421 gkvggepadl rsvvrpfflk lrflvltate fvrgpnvwdm lnaeeslaip cniiekdsla
      481 mtagfctirt qrv
//
LOCUS       XP_077504922             463 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114817 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504922
VERSION     XP_077504922.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648796.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..463
                     /product="uncharacterized protein LOC144114817 isoform X2"
                     /calculated_mol_wt=51789
     Region          207..294
                     /region_name="BTB_POZ_ZBTB_KLHL-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in zinc finger and
                     BTB domain-containing (ZBTB) proteins, Kelch-like (KLHL)
                     proteins, and similar proteins; cd18186"
                     /db_xref="CDD:349497"
     Region          335..415
                     /region_name="BACK"
                     /note="BTB And C-terminal Kelch; smart00875"
                     /db_xref="CDD:197943"
     CDS             1..463
                     /gene="LOC144114817"
                     /coded_by="XM_077648796.1:76..1467"
                     /db_xref="GeneID:144114817"
ORIGIN      
        1 mrhrqamcih nvlpdqsaaq lhrngsklkl amqndlgqra dscletcrni atcatrpwgq
       61 ppdfelakrf valwprstig gqaagdhhhl rgkgevvlkl reapaqkytq pvtavsrppk
      121 pariklwvgq rqsavvslsp aatgqamvll kmyrdgfiyy hvseaatapi ntqctsfklk
      181 elfitfpdil teykvfslwr flgireladv eivvhwehap eqhasfkahr milalqndvf
      241 ramffghfgs gdrvtitdlp pdgvegllry fysgqhqves vhqatcirsa avkymvpela
      301 arcvayvery mepddvcpfl dyiltmggdg vdgpakavlr nnglfvlask tfesclhctv
      361 nyildnvhna pemsvlqavh awgrrqcrer gkvggepadl rsvvrpfflk lrflvltate
      421 fvrgpnvwdm lnaeeslaip cniiekdsla mtagfctirt qrv
//
LOCUS       XP_077504924             157 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114819 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504924
VERSION     XP_077504924.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648798.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..157
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..157
                     /product="uncharacterized protein LOC144114819 isoform X1"
                     /calculated_mol_wt=16203
     CDS             1..157
                     /gene="LOC144114819"
                     /coded_by="XM_077648798.1:1156..1629"
                     /db_xref="GeneID:144114819"
ORIGIN      
        1 mptptsagpa dgsedllvpv akftrpegiv aqvgressai samdadgavl edsveehvdg
       61 vpqspagvss gpspsyfqaa haqampsfei padkwawmmr ntsdskccle iashlrsaee
      121 aatcsltgqs cstmvgassk vpvtpekvka ltdyfsk
//
LOCUS       XP_077504925             157 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114819 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504925
VERSION     XP_077504925.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648799.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..157
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..157
                     /product="uncharacterized protein LOC144114819 isoform X1"
                     /calculated_mol_wt=16203
     CDS             1..157
                     /gene="LOC144114819"
                     /coded_by="XM_077648799.1:880..1353"
                     /db_xref="GeneID:144114819"
ORIGIN      
        1 mptptsagpa dgsedllvpv akftrpegiv aqvgressai samdadgavl edsveehvdg
       61 vpqspagvss gpspsyfqaa haqampsfei padkwawmmr ntsdskccle iashlrsaee
      121 aatcsltgqs cstmvgassk vpvtpekvka ltdyfsk
//
LOCUS       XP_077504926             157 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114819 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504926
VERSION     XP_077504926.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648800.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..157
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..157
                     /product="uncharacterized protein LOC144114819 isoform X1"
                     /calculated_mol_wt=16203
     CDS             1..157
                     /gene="LOC144114819"
                     /coded_by="XM_077648800.1:1111..1584"
                     /db_xref="GeneID:144114819"
ORIGIN      
        1 mptptsagpa dgsedllvpv akftrpegiv aqvgressai samdadgavl edsveehvdg
       61 vpqspagvss gpspsyfqaa haqampsfei padkwawmmr ntsdskccle iashlrsaee
      121 aatcsltgqs cstmvgassk vpvtpekvka ltdyfsk
//
LOCUS       XP_077504927             136 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114819 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504927
VERSION     XP_077504927.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648801.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..136
                     /product="uncharacterized protein LOC144114819 isoform X2"
                     /calculated_mol_wt=14181
     CDS             1..136
                     /gene="LOC144114819"
                     /coded_by="XM_077648801.1:1172..1582"
                     /db_xref="GeneID:144114819"
ORIGIN      
        1 mptptsagpa dgsedllvpv akftrpegiv aqvgressai samdadgavl edsveehvdg
       61 vpqspagvss gpspsyfqaa haqttfrskw rhlapapppe vrvpavrkyl rsffmeagrl
      121 gkcaskkqie gsaipe
//
LOCUS       XP_077504928            1178 aa            linear   INV 25-NOV-2025
DEFINITION  protein fantom-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504928
VERSION     XP_077504928.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648802.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1178
                     /product="protein fantom-like isoform X1"
                     /calculated_mol_wt=131514
     Region          13..>262
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     Region          <170..>417
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          535..675
                     /region_name="C2-C2_1"
                     /note="First C2 domain of RPGR-interacting protein 1;
                     pfam11618"
                     /db_xref="CDD:463310"
     Region          720..830
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          1009..1167
                     /region_name="RPGR1_C"
                     /note="Retinitis pigmentosa G-protein regulator
                     interacting C-terminal; pfam18111"
                     /db_xref="CDD:465655"
     CDS             1..1178
                     /gene="LOC144114820"
                     /coded_by="XM_077648802.1:176..3712"
                     /db_xref="GeneID:144114820"
ORIGIN      
        1 mgsarldgea fgrrsleydy lqlrdenvhl kkhareqeek akklatrlsr vinekikega
       61 apndlagprr qmvemeqkve dlgdkvrvle kqnmqlkgkl qvsrtlmata aqyhgsygkv
      121 qprvnsglrr pvqkphpfra eplrtavslq kkmaasdlvh appyvvalvk eakeeiekle
      181 tlvesqrlql aeaseqarfr eaelqtelse lkeqvnhgqv ryiqgnvdil glrrdaehya
      241 lresalksel ealdkkmeki saynssllge vdqltselkt eqaknlqlee klhaceamqk
      301 tlaeleqrlv diqqeneilk tandqllnsi egtdtdenqs leeartlrlq itqleaiiks
      361 dndekaalek elvervelyq rlqeehvnle kahaevttrl etvqkdlqnl qeeahkksls
      421 vnkimqpdss vnawggvltn gfspstpgss kkfldkevqa tllessyqem tklkeeqqli
      481 ieeerlrine lqsllkeksk kiealekrla lipskqvqlt evcapneadd ehclslvrrg
      541 qsfleihlnk itfasrqeas dsnayppvfa twtfadfqpq stpvatgdrp efnctaryav
      601 tvdsdfldyl dkgsvhiqvq svhgakvqnv gtcqislrpa vqepfkrsss qaaligiqdg
      661 qrkhfvgtls cwvrflpapd pakrflnfsv psspakrlpn svvehqserq ngepasamyn
      721 qlnvnivrci giplkqgqmr krvyccyqlc dlpvhetgla tavancadfh hvnrfpvmvd
      781 sqlhkqllsh sivllvfdee daepgsfigk asvplsaltl gqalhgsfpl vnpwgedags
      841 vdieikweqe ytppkkpvpr arsrdaihqp rkvssarqra atlhfsptts glskdfqvpq
      901 rprrsavner kkmlstrkgn resgertess sltsvsslss agtangtrgm edskdqkpea
      961 gsstagvkss iveesqsiee fsngqvvvvk tpalkrsgtf kvsdvavfsv nnlgflpgaa
     1021 vlrnksvemv fvefqfldcs peeletpval pkpkppqria fnfrkvvhld atrhqerrea
     1081 lasmlasenp nsatikftvv seplgkkkda ecqdvgfait dlkqivkygk diieedvdvl
     1141 dtrsnkvigk lnvsvemlaa lkhilveskh asrvplqp
//
LOCUS       XP_077504929            1177 aa            linear   INV 25-NOV-2025
DEFINITION  protein fantom-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504929
VERSION     XP_077504929.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648803.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1177
                     /product="protein fantom-like isoform X2"
                     /calculated_mol_wt=131443
     Region          <13..509
                     /region_name="PRK03918"
                     /note="DNA double-strand break repair ATPase Rad50"
                     /db_xref="CDD:235175"
     Region          <169..>416
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          534..674
                     /region_name="C2-C2_1"
                     /note="First C2 domain of RPGR-interacting protein 1;
                     pfam11618"
                     /db_xref="CDD:463310"
     Region          719..829
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          1008..1166
                     /region_name="RPGR1_C"
                     /note="Retinitis pigmentosa G-protein regulator
                     interacting C-terminal; pfam18111"
                     /db_xref="CDD:465655"
     CDS             1..1177
                     /gene="LOC144114820"
                     /coded_by="XM_077648803.1:176..3709"
                     /db_xref="GeneID:144114820"
ORIGIN      
        1 mgsarldgea fgrrsleydy lqlrdenvhl kkhareqeek akklatrlsr vinekikega
       61 apndlgprrq mvemeqkved lgdkvrvlek qnmqlkgklq vsrtlmataa qyhgsygkvq
      121 prvnsglrrp vqkphpfrae plrtavslqk kmaasdlvha ppyvvalvke akeeieklet
      181 lvesqrlqla easeqarfre aelqtelsel keqvnhgqvr yiqgnvdilg lrrdaehyal
      241 resalksele aldkkmekis aynssllgev dqltselkte qaknlqleek lhaceamqkt
      301 laeleqrlvd iqqeneilkt andqllnsie gtdtdenqsl eeartlrlqi tqleaiiksd
      361 ndekaaleke lvervelyqr lqeehvnlek ahaevttrle tvqkdlqnlq eeahkkslsv
      421 nkimqpdssv nawggvltng fspstpgssk kfldkevqat llessyqemt klkeeqqlii
      481 eeerlrinel qsllkekskk iealekrlal ipskqvqlte vcapneadde hclslvrrgq
      541 sfleihlnki tfasrqeasd snayppvfat wtfadfqpqs tpvatgdrpe fnctaryavt
      601 vdsdfldyld kgsvhiqvqs vhgakvqnvg tcqislrpav qepfkrsssq aaligiqdgq
      661 rkhfvgtlsc wvrflpapdp akrflnfsvp sspakrlpns vvehqserqn gepasamynq
      721 lnvnivrcig iplkqgqmrk rvyccyqlcd lpvhetglat avancadfhh vnrfpvmvds
      781 qlhkqllshs ivllvfdeed aepgsfigka svplsaltlg qalhgsfplv npwgedagsv
      841 dieikweqey tppkkpvpra rsrdaihqpr kvssarqraa tlhfspttsg lskdfqvpqr
      901 prrsavnerk kmlstrkgnr esgertesss ltsvsslssa gtangtrgme dskdqkpeag
      961 sstagvkssi veesqsieef sngqvvvvkt palkrsgtfk vsdvavfsvn nlgflpgaav
     1021 lrnksvemvf vefqfldcsp eeletpvalp kpkppqriaf nfrkvvhlda trhqerreal
     1081 asmlasenpn satikftvvs eplgkkkdae cqdvgfaitd lkqivkygkd iieedvdvld
     1141 trsnkvigkl nvsvemlaal khilveskha srvplqp
//
LOCUS       XP_077504930            1177 aa            linear   INV 25-NOV-2025
DEFINITION  protein fantom-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077504930
VERSION     XP_077504930.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648804.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1177
                     /product="protein fantom-like isoform X3"
                     /calculated_mol_wt=131401
     Region          13..>262
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     Region          <170..>417
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          535..675
                     /region_name="C2-C2_1"
                     /note="First C2 domain of RPGR-interacting protein 1;
                     pfam11618"
                     /db_xref="CDD:463310"
     Region          720..830
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          1008..1166
                     /region_name="RPGR1_C"
                     /note="Retinitis pigmentosa G-protein regulator
                     interacting C-terminal; pfam18111"
                     /db_xref="CDD:465655"
     CDS             1..1177
                     /gene="LOC144114820"
                     /coded_by="XM_077648804.1:176..3709"
                     /db_xref="GeneID:144114820"
ORIGIN      
        1 mgsarldgea fgrrsleydy lqlrdenvhl kkhareqeek akklatrlsr vinekikega
       61 apndlagprr qmvemeqkve dlgdkvrvle kqnmqlkgkl qvsrtlmata aqyhgsygkv
      121 qprvnsglrr pvqkphpfra eplrtavslq kkmaasdlvh appyvvalvk eakeeiekle
      181 tlvesqrlql aeaseqarfr eaelqtelse lkeqvnhgqv ryiqgnvdil glrrdaehya
      241 lresalksel ealdkkmeki saynssllge vdqltselkt eqaknlqlee klhaceamqk
      301 tlaeleqrlv diqqeneilk tandqllnsi egtdtdenqs leeartlrlq itqleaiiks
      361 dndekaalek elvervelyq rlqeehvnle kahaevttrl etvqkdlqnl qeeahkksls
      421 vnkimqpdss vnawggvltn gfspstpgss kkfldkevqa tllessyqem tklkeeqqli
      481 ieeerlrine lqsllkeksk kiealekrla lipskqvqlt evcapneadd ehclslvrrg
      541 qsfleihlnk itfasrqeas dsnayppvfa twtfadfqpq stpvatgdrp efnctaryav
      601 tvdsdfldyl dkgsvhiqvq svhgakvqnv gtcqislrpa vqepfkrsss qaaligiqdg
      661 qrkhfvgtls cwvrflpapd pakrflnfsv psspakrlpn svvehqserq ngepasamyn
      721 qlnvnivrci giplkqgqmr krvyccyqlc dlpvhetgla tavancadfh hvnrfpvmvd
      781 sqlhkqllsh sivllvfdee daepgsfigk asvplsaltl gqalhgsfpl vnpwgedags
      841 vdieikweqe ytppkkpvpr arsrdaihqp rkvssarqra atlhfsptts glskdfqvpq
      901 rprrsavner kkmstrkgnr esgertesss ltsvsslssa gtangtrgme dskdqkpeag
      961 sstagvkssi veesqsieef sngqvvvvkt palkrsgtfk vsdvavfsvn nlgflpgaav
     1021 lrnksvemvf vefqfldcsp eeletpvalp kpkppqriaf nfrkvvhlda trhqerreal
     1081 asmlasenpn satikftvvs eplgkkkdae cqdvgfaitd lkqivkygkd iieedvdvld
     1141 trsnkvigkl nvsvemlaal khilveskha srvplqp
//
LOCUS       XP_077504931             203 aa            linear   INV 25-NOV-2025
DEFINITION  signal peptidase complex subunit Spase25 [Amblyomma americanum].
ACCESSION   XP_077504931
VERSION     XP_077504931.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648805.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..203
                     /product="signal peptidase complex subunit Spase25"
                     /calculated_mol_wt=22820
     Region          38..194
                     /region_name="SPC25"
                     /note="Microsomal signal peptidase 25 kDa subunit (SPC25);
                     pfam06703"
                     /db_xref="CDD:461993"
     CDS             1..203
                     /gene="Spase25"
                     /coded_by="XM_077648805.1:103..714"
                     /db_xref="GeneID:144114821"
ORIGIN      
        1 maskrvqdag dtsgaekeae kpikidkwdg savrnaldda vkkvltekyc yvenhrltdg
       61 rlaicsiavg aamfallwdf lypfpesrtv liacvlsyff lmgvltlytt flekgiflva
      121 lqkdktgfdp dsvwtvsssl krfdhiyhlq ihykdgktkk treakleksi aswfdengtl
      181 iadlfepevc klhnsllsak kdk
//
LOCUS       XP_077504932             828 aa            linear   INV 25-NOV-2025
DEFINITION  ribosome biogenesis protein bop1-B [Amblyomma americanum].
ACCESSION   XP_077504932
VERSION     XP_077504932.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648806.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..828
                     /product="ribosome biogenesis protein bop1-B"
                     /calculated_mol_wt=93188
     Region          <6..>140
                     /region_name="PHA03169"
                     /note="hypothetical protein; Provisional"
                     /db_xref="CDD:223003"
     Region          218..475
                     /region_name="BOP1NT"
                     /note="BOP1NT (NUC169) domain; pfam08145"
                     /db_xref="CDD:462371"
     Region          <481..826
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          491..526
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          531..612
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          617..657
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          665..699
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          705..742
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          748..793
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          803..827
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..828
                     /gene="LOC144114822"
                     /coded_by="XM_077648806.1:94..2580"
                     /db_xref="GeneID:144114822"
ORIGIN      
        1 maakrataka sgksgskgte avrnqesamn kpeqhesdes dssvysglgs ddddsselde
       61 saeeadegdv ssedggddss elhesaeedd egdvvssedg gdgssgtdde rgdeftyepd
      121 saeqvsddea tddesedeea mkmkfiggkg ddfdlqkkrk shypevshgk rssevskeed
      181 vkidalgtkv akgdeyayds sdeedvrntv gnipmewyeh yphigydldg kpilkpprgt
      241 eldeflrkmd dpdywrtvkd kstgqnvvls dedvdliqrl qqgrypsgsy dpyepfedif
      301 shetmihpvt shppqkrsfv pskiekqmvs rmvhaikmgw ikprvkrhdl drfsllwdkd
      361 deekpndrmq ryipapkmkl pgheesynpp seylftkeee ekwraqepee rrisfiprky
      421 sslrlvpayd rfinerferc ldlylcprqr kmrvnvdaed lipqlpkpkd lqpfpsvqsi
      481 vyeghadcvr slsiepagqf fasgsddgtv riwevltgrc lktfkfehpv kgvawcpnqs
      541 iclvavavek ivhvinpgvg dklvlsntdt vlsslpeets psegakftaa nwevasptsk
      601 eysskglrls vvhrhtvsqv twhakgdyfa vvltggsgge sgvvihqlsh rrsqapfskl
      661 kgtvsrvlfh pmrpyffvam qrfvrvynll kqelskklis nckwissiav hpkgdnvitg
      721 sfenrltwfd mdlsvkpykt lrhhkgairq vafhrryplf asasddgtvi ishgmvysdl
      781 mqnplivpvk ilrghtvqks mgvldcafht qqpwiitaga dgtlrlfs
//
LOCUS       XP_077504933            1180 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger protein ush isoform X1 [Amblyomma americanum].
ACCESSION   XP_077504933
VERSION     XP_077504933.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648807.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1180
                     /product="zinc finger protein ush isoform X1"
                     /calculated_mol_wt=125447
     Region          27..144
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(45..46,102..107,118..120,141..144)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Site            order(45..46,103..107,143)
                     /site_type="other"
                     /note="SAM binding site [polypeptide binding]"
                     /db_xref="CDD:380914"
     Region          433..455
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          435..455
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          845..864
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(850,852,854,856..857,860..861,864,876,882..883,
                     885..886,889,903,905,907,909..910,913..914,917)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          869..>925
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          870..895
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          870..890
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(880,892,894,897,904)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          898..918
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          898..918
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1180
                     /gene="ush"
                     /coded_by="XM_077648807.1:711..4253"
                     /db_xref="GeneID:144114824"
ORIGIN      
        1 msrrkqsnpk plkrkaeeel eelaeslqlp sevlalreag epspelsvyt rvmlrggtlf
       61 gpyaahlvkd pgqsvsaahi tihnkegsat ylklkeaags wlktlrlvqe kesanaslsl
      121 egdevwcnlt rdiaadtell ahftasskdk qiaprtetaa dsdalgddid vvvddtdnve
      181 sassaaeelk ngvrspvtti ssspvpttcn gpnaslvadd eqkmsveaen tselakraes
      241 lsevtvqsse lthvqvklev cspaavasgr tsedlvsdda dgessatknd hlaavaskkt
      301 dlqpehingy splsspamll qrpaivlqqa fecsmcgiqf ssgktlkahq tyycarrgek
      361 aafvparpqt evaedcppsv spgqrsspre ngeagmepci vpakrprred glsppssdas
      421 sttskspksg rtyqckycsy aydrkgsltr hmrlhgspss ptnttdappt aaaedisipp
      481 sarycancdi qfssyktftv hkqfycgtrh vqkapvsiap apppaptvsv apaapeqvll
      541 nqplfaaist nplilvpcsy vpgnglvptt ggvlrqdasp avapaivcpq tvtvapaamv
      601 tssgqatpvt stpaepceak essshckelg segtagtgan dsksnspcik reqvtetpld
      661 lslrkeakpg qsprdpsspt pvqqeeapnp ssivvgamdp lmtlgeqvvv kqgtsrcrec
      721 nivfykheny lahkrhycas rqqklgrlss psgdegsssd lasrspsana saspvppvpd
      781 mfgqrgagvq sppqplyqfy clacgikfts lsnlqahqty ycpkrdvlkg qvgvaavtrp
      841 aefscarcrl syptdealkq hlcsaalrkc pycdvfcptq iaaqrhlvth tgvrafrcae
      901 cgykghtlrg mrthvrmhle kgtqvqeeal ilcvgadgnt lcpwngttgv paavtptipr
      961 asetteetsa rsgspptata mteesnstsr acedhhpapt ellhwcnlcg ysssykgnvv
     1021 rhvklvhrdv vatsavhsft serprpsine rrhspvpevk veevsdaedt tteigatqqs
     1081 eamtcvspav aetsatlvdv vakssadada saamkpvlpl ptpkgkvpgg tkyckacdis
     1141 fnylstfvah kkyyctsqts dsadslsavt elgesapgta
//
LOCUS       XP_077504934            1128 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger protein ush isoform X2 [Amblyomma americanum].
ACCESSION   XP_077504934
VERSION     XP_077504934.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648808.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1128
                     /product="zinc finger protein ush isoform X2"
                     /calculated_mol_wt=119545
     Region          <2..92
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(50..55,66..68,89..92)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Region          381..403
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          383..403
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          793..812
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(798,800,802,804..805,808..809,812,824,830..831,
                     833..834,837,851,853,855,857..858,861..862,865)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          817..>873
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          818..843
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          818..838
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(828,840,842,845,852)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          846..866
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          846..866
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1128
                     /gene="ush"
                     /coded_by="XM_077648808.1:924..4310"
                     /db_xref="GeneID:144114824"
ORIGIN      
        1 mlrggtlfgp yaahlvkdpg qsvsaahiti hnkegsatyl klkeaagswl ktlrlvqeke
       61 sanaslsleg devwcnltrd iaadtellah ftasskdkqi aprtetaads dalgddidvv
      121 vddtdnvesa ssaaeelkng vrspvttiss spvpttcngp naslvaddeq kmsveaents
      181 elakraesls evtvqsselt hvqvklevcs paavasgrts edlvsddadg essatkndhl
      241 aavaskktdl qpehingysp lsspamllqr paivlqqafe csmcgiqfss gktlkahqty
      301 ycarrgekaa fvparpqtev aedcppsvsp gqrsspreng eagmepcivp akrprredgl
      361 sppssdasst tskspksgrt yqckycsyay drkgsltrhm rlhgspsspt nttdapptaa
      421 aedisippsa rycancdiqf ssyktftvhk qfycgtrhvq kapvsiapap ppaptvsvap
      481 aapeqvllnq plfaaistnp lilvpcsyvp gnglvpttgg vlrqdaspav apaivcpqtv
      541 tvapaamvts sgqatpvtst paepceakes sshckelgse gtagtgands ksnspcikre
      601 qvtetpldls lrkeakpgqs prdpssptpv qqeeapnpss ivvgamdplm tlgeqvvvkq
      661 gtsrcrecni vfykhenyla hkrhycasrq qklgrlssps gdegsssdla srspsanasa
      721 spvppvpdmf gqrgagvqsp pqplyqfycl acgikftsls nlqahqtyyc pkrdvlkgqv
      781 gvaavtrpae fscarcrlsy ptdealkqhl csaalrkcpy cdvfcptqia aqrhlvthtg
      841 vrafrcaecg ykghtlrgmr thvrmhlekg tqvqeealil cvgadgntlc pwngttgvpa
      901 avtptipras etteetsars gspptatamt eesnstsrac edhhpaptel lhwcnlcgys
      961 ssykgnvvrh vklvhrdvva tsavhsftse rprpsinerr hspvpevkve evsdaedttt
     1021 eigatqqsea mtcvspavae tsatlvdvva kssadadasa amkpvlplpt pkgkvpggtk
     1081 yckacdisfn ylstfvahkk yyctsqtsds adslsavtel gesapgta
//
LOCUS       XP_077504935            1095 aa            linear   INV 25-NOV-2025
DEFINITION  importin-4-like [Amblyomma americanum].
ACCESSION   XP_077504935
VERSION     XP_077504935.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648809.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1095
                     /product="importin-4-like"
                     /calculated_mol_wt=120126
     Region          23..91
                     /region_name="IBN_N"
                     /note="Importin-beta N-terminal domain; smart00913"
                     /db_xref="CDD:197981"
     Region          351..377
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          362..417
                     /region_name="HEAT_EZ"
                     /note="HEAT-like repeat; pfam13513"
                     /db_xref="CDD:463906"
     Site            order(366..367,370,373..374,377,408..409,412,415..416,419,
                     460..461,462,465..466,499..500,503,506..507)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          390..420
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          431..466
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          479..507
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          520..553
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          872..897
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(889..890,893,896..897,931..932,935,938..939,944,
                     973..974,977,980..981,984,1015..1016,1019,1022..1023)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          914..944
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          928..982
                     /region_name="HEAT_EZ"
                     /note="HEAT-like repeat; pfam13513"
                     /db_xref="CDD:463906"
     Region          954..985
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          994..1023
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..1095
                     /gene="LOC144114825"
                     /coded_by="XM_077648809.1:49..3336"
                     /db_xref="GeneID:144114825"
ORIGIN      
        1 maaslesill rllvpdnavi aqatqelrel ykdpniiehl lgalcksqsv qvrqysavll
       61 rkkicrlkaw rklpqdkrng lkcvllqrit serekavvla igqlvaviak hecqigpwse
      121 lqellnvlmn sknveecrlg fyivsvvtse apemflghmk qlfnlfgscl qsfadehlcf
      181 yviksmtalv sslgsedsnc fqvlipyvle virrlvkvse ekatealelf delidseisi
      241 llphikplik mcleiasdsk sgdvlrvram svlswminvk kktivkhkli peilevlfpi
      301 meevsagdld senededder ycqspsacaa qlidtmalhl ppekllppls qhvekflass
      361 npfqkraayl aiaviaegcs eairqkhlpm fvhvicqgvl hedvhvrnaa lfalgqfadf
      421 lqpeigkyan eilpillnhl gqtaeqmaqt gkdppnlskt fyaletfcen legelvfylp
      481 avmervlvfl tapsyrakel aisaigsaan atkeamlpyf phvidnlkqy ltehqsehna
      541 tlrtqavdtl gclartigaa nflpmaedcv qlglrlidav ddpdlrrsty gmfasvssvl
      601 kadmtkylep ildhmftslq stegvvtrpv naaghglelf egleesddea aeidttgggd
      661 asedddddde qgytvenayl eekedtcvti aelaenigpa fapylekcft evlpmashpa
      721 gdirkaalsc mgqltvvlfk satdasgggs vtiadvskat gmlipklvev srteqerevv
      781 laaletltll inelkiaafe npkhindivs lvkdafndkl asqdadsdge daeeqdeaey
      841 dgllvqmage lvptlakalp aeqfapylag llpfffgklk kqnstsdksy avgtlaevaq
      901 glgqagigpf ckpllsvfla gmrdrdsevr snavfglgvl iqnapdilap eypsllgals
      961 smlarednrh akdnicgava rlilvgansv pmdqvfpvll qqlpleedfe envsvfqcic
     1021 ylygckhqel iknlpsilrl ilrvigtdqv nadtraslvl llksmsseip nevqqvlqac
     1081 tpqeretfna vlsgs
//
LOCUS       XP_077504937             437 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114826 [Amblyomma americanum].
ACCESSION   XP_077504937
VERSION     XP_077504937.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648811.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..437
                     /product="uncharacterized protein LOC144114826"
                     /calculated_mol_wt=47179
     CDS             1..437
                     /gene="LOC144114826"
                     /coded_by="XM_077648811.1:105..1418"
                     /db_xref="GeneID:144114826"
ORIGIN      
        1 mtdekspeqq rrqtssmass pvgrassdrr pvqaeearsk aassyalpcp pdsrrrvsrd
       61 alskslgdyr ymavpatgra scvalghypq vareagltpg rafgppevgs yyvpehpyap
      121 gyvaypamgs yelkqgrrpq aeptaagpqd nqntahhavv tqvgmmhqag dvagpttala
      181 agkqqgspvs pgeksaesdw sptkelmked arsaiyyagt asegsseqrd krdessgssi
      241 stasssgssd tsaepepqki pppteqvpls qhlmhmqqlq kmqdmqemqq mqqmqqmhhq
      301 lqqmrqhmqq qqyqqqmqql qqsmsdqddp qkpapqqapr lkdpraesag raaasvapag
      361 pprgyffrpa rengshtkqt esrsqfsvvs laapllpasa aslanrsame qacilvnvvl
      421 lsvltgivaa flvrqlt
//
LOCUS       XP_077504938             437 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114826 [Amblyomma americanum].
ACCESSION   XP_077504938
VERSION     XP_077504938.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648812.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..437
                     /product="uncharacterized protein LOC144114826"
                     /calculated_mol_wt=47179
     CDS             1..437
                     /gene="LOC144114826"
                     /coded_by="XM_077648812.1:42..1355"
                     /db_xref="GeneID:144114826"
ORIGIN      
        1 mtdekspeqq rrqtssmass pvgrassdrr pvqaeearsk aassyalpcp pdsrrrvsrd
       61 alskslgdyr ymavpatgra scvalghypq vareagltpg rafgppevgs yyvpehpyap
      121 gyvaypamgs yelkqgrrpq aeptaagpqd nqntahhavv tqvgmmhqag dvagpttala
      181 agkqqgspvs pgeksaesdw sptkelmked arsaiyyagt asegsseqrd krdessgssi
      241 stasssgssd tsaepepqki pppteqvpls qhlmhmqqlq kmqdmqemqq mqqmqqmhhq
      301 lqqmrqhmqq qqyqqqmqql qqsmsdqddp qkpapqqapr lkdpraesag raaasvapag
      361 pprgyffrpa rengshtkqt esrsqfsvvs laapllpasa aslanrsame qacilvnvvl
      421 lsvltgivaa flvrqlt
//
LOCUS       XP_077504939             530 aa            linear   INV 25-NOV-2025
DEFINITION  DNA-dependent metalloprotease SPRTN-like [Amblyomma americanum].
ACCESSION   XP_077504939
VERSION     XP_077504939.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648813.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..530
                     /product="DNA-dependent metalloprotease SPRTN-like"
                     /calculated_mol_wt=58762
     Region          64..232
                     /region_name="SprT"
                     /note="SprT homologues; smart00731"
                     /db_xref="CDD:214794"
     Region          503..526
                     /region_name="ZnF_Rad18"
                     /note="Rad18-like CCHC zinc finger; smart00734"
                     /db_xref="CDD:128973"
     CDS             1..530
                     /gene="LOC144114827"
                     /coded_by="XM_077648813.1:194..1786"
                     /db_xref="GeneID:144114827"
ORIGIN      
        1 msmasedeee lalemaleha yhvsllewne psieepaats vathslrkdl svvdpfwqqc
       61 dpypdvqrlf vefneayfyg kldqvavswn whmtlcaglc eyegdglctv klskpllkfr
      121 trkdlvetll hemihaylfv tdgnmdhdah gpefhkhmdr vnkasganit vfhnfheeln
      181 nyrthwwrcn grcqhrfpff givrramnrp psdydpwwad hqricggtft kirepdpkgs
      241 kgkrkdgtlp vkndakqpkl mdgadirapl vpphsvkstv svpdsqshqg trpkhvssfp
      301 ntmtklpsnv htlpqhatdl qwpqvvpftg eghrlggcss ksrllgesnc vlggvssqml
      361 hidaneatsw ssavsgsgqs lskcvtetqv kpapqkpsds twptlkdfld qktqpaeqts
      421 tksstakmkl rdlfgsdifg sdnfgsdtes dddcvitead mtvkeqgkct gkldeasass
      481 sssltstdvg atanvstrsv valiecpvcg hfllekfins hldtclipdd
//
LOCUS       XP_077504940             261 aa            linear   INV 25-NOV-2025
DEFINITION  yip1 domain-containing 1 [Amblyomma americanum].
ACCESSION   XP_077504940
VERSION     XP_077504940.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648814.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..261
                     /product="yip1 domain-containing 1"
                     /calculated_mol_wt=27536
     Region          76..261
                     /region_name="YIP1"
                     /note="Rab GTPase interacting factor, Golgi membrane
                     protein [Intracellular trafficking and secretion];
                     cl46908"
                     /db_xref="CDD:481248"
     CDS             1..261
                     /gene="Yip1d1"
                     /coded_by="XM_077648814.1:76..861"
                     /db_xref="GeneID:144114828"
ORIGIN      
        1 msnygpnqdf yydwnnpppv qqgymydtgs qhdfgaqqqf anfdygapsg gvsapgygnp
       61 ssyvgagspy tgsiltpdss lgsygsdddf enepplleel ginfdhilqk tlavlnpmkd
      121 pdaailqetd lagplvfcla fggfllltgk vhfgyiygig vlgclamysl lnlmsasgis
      181 vgctvsvlgy cllpmvllsg lsilfslkgs lgtavalavv vwcalsaskl fvtalsmvhq
      241 qplvlypcal vygvfallti f
//
LOCUS       XP_077504941             526 aa            linear   INV 25-NOV-2025
DEFINITION  DNA-dependent metalloprotease dvc-1-like [Amblyomma americanum].
ACCESSION   XP_077504941
VERSION     XP_077504941.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648815.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..526
                     /product="DNA-dependent metalloprotease dvc-1-like"
                     /calculated_mol_wt=58425
     Region          64..232
                     /region_name="SprT"
                     /note="SprT homologues; smart00731"
                     /db_xref="CDD:214794"
     Region          499..522
                     /region_name="ZnF_Rad18"
                     /note="Rad18-like CCHC zinc finger; smart00734"
                     /db_xref="CDD:128973"
     CDS             1..526
                     /gene="LOC144114829"
                     /coded_by="XM_077648815.1:220..1800"
                     /db_xref="GeneID:144114829"
ORIGIN      
        1 msmasedeee lalemgeeha fhvsllklne psieepaats vathslqkdl svvdpfwelc
       61 dpypdvqrlf fefneayfyg kldqvavswn whmtlcaglc eyegdglctv klsepllklr
      121 trkdlvetll hemihaylfv tdgnmdhdah gpefhkhmdr vnkasganit vfhnfheeln
      181 nyrthwwrcn grcqhrfpff givrramnrp psdydrwwad hqrncggtft kirepdpkgs
      241 kgkrkdgtlp vkndakqpkl mdgadirapl vpphsvkstv svpdsqshqg trpkhvssfp
      301 ntmtklpsnv htlpqhatdl qwpqvvpftg egrrlggcss ksrllgesnc vlggvssqml
      361 hidaneatsw ssavsgpgqs lskcvtetqv kpapqkpsds twptlkdfld qktqpaeqts
      421 tksstakmkf krdlfgsdvf gsdtesdddc viteadmtvk eqgkctgkld eastsssssl
      481 tstdvgatan vstrsvvali ecpvcghfll ekfinshldt cliqdd
//
LOCUS       XP_077504942             310 aa            linear   INV 25-NOV-2025
DEFINITION  alanine--glyoxylate aminotransferase 2, mitochondrial-like
            [Amblyomma americanum].
ACCESSION   XP_077504942
VERSION     XP_077504942.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648816.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..310
                     /product="alanine--glyoxylate aminotransferase 2,
                     mitochondrial-like"
                     /calculated_mol_wt=33050
     Region          <11..306
                     /region_name="ArgD"
                     /note="Acetylornithine aminotransferase/4-aminobutyrate
                     aminotransferase [Amino acid transport and metabolism];
                     COG0160"
                     /db_xref="CDD:439930"
     CDS             1..310
                     /gene="LOC144114830"
                     /coded_by="XM_077648816.1:24..956"
                     /db_xref="GeneID:144114830"
ORIGIN      
        1 mspycaslcg vgnykhhips sfgtfhamnp dpyrgvwgga kcrdspvqtd rtcscgtece
       61 aasryadqls evlqqsvskk kvaaffaesi qgvggsvqyp kgylkkahkl ikdkgglfva
      121 devqtgfgrt gdhywgfegh gimpdivtma kgigngfpla avittpeisk aldaasffnt
      181 fggnpvatav gsavvevldd egcmenarvm gnrllqales lrkefpvigd vrgkglmlgv
      241 elvenketkv plgaekvnai lehcremgvl igkggqyghv lrikppmcit eadvdftaav
      301 lnraiahvqq
//
LOCUS       XP_077504943             492 aa            linear   INV 25-NOV-2025
DEFINITION  alanine--glyoxylate aminotransferase 2, mitochondrial [Amblyomma
            americanum].
ACCESSION   XP_077504943
VERSION     XP_077504943.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648817.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..492
                     /product="alanine--glyoxylate aminotransferase 2,
                     mitochondrial"
                     /calculated_mol_wt=53526
     Region          45..488
                     /region_name="ArgD"
                     /note="Acetylornithine aminotransferase/4-aminobutyrate
                     aminotransferase [Amino acid transport and metabolism];
                     COG0160"
                     /db_xref="CDD:439930"
     CDS             1..492
                     /gene="LOC144114831"
                     /coded_by="XM_077648817.1:508..1986"
                     /db_xref="GeneID:144114831"
ORIGIN      
        1 mllkvslfra fssqcgiksa rpltsvpeip pcsekpkpyq grpwaeival rkqhlnpsls
       61 snllykepll ltqghmqwlw dhtgkryldm fagivtvsvg hchpkvvaaa esqlkqlwht
      121 snvylyptvh efaeklsskm pgdlkvcyft nsgseandla mmmarlytga fdiislrnay
      181 hgmspycasl cgvgnykhhi pssfgtfham npdpyrgvwg gakcrdspvq tdrtcscgte
      241 ceaasryadq lsevlqqsvs kkkvaaffae siqgvggsvq ypkgylkkah klikdkgglf
      301 vadevqtgfg rtgdhywgfe ghgimpdivt makgigngfp laavittpei skaldaasff
      361 ntfggnpvat avgsavvevl ddegcmenar vmgnrllqal eslrkefpvi gdvrgkglml
      421 gvelvenket kvplgaekvn ailehcremg vligkggqyg hvlrikppmc iteadvdfta
      481 avlkraiahv qq
//
LOCUS       XP_077504944             289 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114832 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504944
VERSION     XP_077504944.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648818.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..289
                     /product="uncharacterized protein LOC144114832 isoform X1"
                     /calculated_mol_wt=32010
     Region          <141..>239
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..289
                     /gene="LOC144114832"
                     /coded_by="XM_077648818.1:157..1026"
                     /db_xref="GeneID:144114832"
ORIGIN      
        1 maassvkesd drssvcedvn pfsfqrfvni rsssttehsa sdklanliag gqhgrqthrk
       61 clvvvddkpk eavahikrpq sldliahlga vparrpslsd ttdascpsss eqigaaesnw
      121 vqepppvepg ahchldkdlt erldeknkql qkqledaqkv nkeqlrrlks leeqleqqqa
      181 rerdetaale rmiqqvecsl kaaqerasda eqlahslkle vkalqarckq leqqqhdslp
      241 emtsdlsckl raaadsaegs lrslldgvsq lrlysqvles wtkvhedps
//
LOCUS       XP_077504945             288 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114832 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504945
VERSION     XP_077504945.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648819.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..288
                     /product="uncharacterized protein LOC144114832 isoform X2"
                     /calculated_mol_wt=31881
     Region          <140..>238
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..288
                     /gene="LOC144114832"
                     /coded_by="XM_077648819.1:155..1021"
                     /db_xref="GeneID:144114832"
ORIGIN      
        1 maassvkesd drssvcedvn pfsfqrfvni rsssttehsa sdklanliag gqhgrqthrk
       61 clvvvddkpk avahikrpqs ldliahlgav parrpslsdt tdascpssse qigaaesnwv
      121 qepppvepga hchldkdlte rldeknkqlq kqledaqkvn keqlrrlksl eeqleqqqar
      181 erdetaaler miqqvecslk aaqerasdae qlahslklev kalqarckql eqqqhdslpe
      241 mtsdlscklr aaadsaegsl rslldgvsql rlysqvlesw tkvhedps
//
LOCUS       XP_077504946             287 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114832 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504946
VERSION     XP_077504946.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648820.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..287
                     /product="uncharacterized protein LOC144114832 isoform X3"
                     /calculated_mol_wt=31810
     Region          <139..>237
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..287
                     /gene="LOC144114832"
                     /coded_by="XM_077648820.1:157..1020"
                     /db_xref="GeneID:144114832"
ORIGIN      
        1 maassvkesd drssvcedvn pfsfqrfvni rsssttehsa sdklanliag gqhgrqthrk
       61 clvvvddkpk vahikrpqsl dliahlgavp arrpslsdtt dascpssseq igaaesnwvq
      121 epppvepgah chldkdlter ldeknkqlqk qledaqkvnk eqlrrlksle eqleqqqare
      181 rdetaalerm iqqvecslka aqerasdaeq lahslklevk alqarckqle qqqhdslpem
      241 tsdlscklra aadsaegslr slldgvsqlr lysqvleswt kvhedps
//
LOCUS       XP_077504947             310 aa            linear   INV 25-NOV-2025
DEFINITION  protein phosphatase 1 regulatory subunit 3E-like [Amblyomma
            americanum].
ACCESSION   XP_077504947
VERSION     XP_077504947.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648821.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..310
                     /product="protein phosphatase 1 regulatory subunit
                     3E-like"
                     /calculated_mol_wt=34459
     Region          177..276
                     /region_name="CBM_21"
                     /note="Carbohydrate/starch-binding module (family 21);
                     pfam03370"
                     /db_xref="CDD:427265"
     CDS             1..310
                     /gene="LOC144114834"
                     /coded_by="XM_077648821.1:343..1275"
                     /db_xref="GeneID:144114834"
ORIGIN      
        1 msflsrsart satamsktca pppgerrefa nvlnaadads iylylprnls schmcaevee
       61 qhwerkmkll aeflkresle sgdddvlsmk pippadgavr gilrrgppkk spagwterrr
      121 rgrlvtfada lglelehvrr lvhrirtpqp apstappvle peftlasghe lrqrvhrdkv
      181 clsalsvrel tlhgtvsvan ltyhkhvfvr yttnewqshv dwpasylpgs ladgvdqfaf
      241 alsvadaplr celavcfete dgrrywdnng ganyrfrrsr tapaaaaaav drapaahksa
      301 sqcgswlhwl
//
LOCUS       XP_077504949             384 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114835 [Amblyomma americanum].
ACCESSION   XP_077504949
VERSION     XP_077504949.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648823.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..384
                     /product="uncharacterized protein LOC144114835"
                     /calculated_mol_wt=42568
     CDS             1..384
                     /gene="LOC144114835"
                     /coded_by="XM_077648823.1:1274..2428"
                     /db_xref="GeneID:144114835"
ORIGIN      
        1 mqvhspsiiw lllnavfcat wataierapl rgaadgrtlc aattnppiqv nltlsadwea
       61 pssattltvp sikeqrpsta pcglggcatt rlhnpllfam qvgnhqtift kktsnyflvq
      121 lpspqcclat ciecfhvvrs lllmcgdies npgpekldvi lgelkkmstg qtelvkevqe
      181 lkgqllsidl kmtnlttrls aletdyesls tlrndlegiq atsaatsrlv gvlearldda
      241 enrsrrnnll fyglpdtnpt etysltedli irhcsdhldt qlspndiera hrlgrhskdr
      301 krpiivkfts fktkesilsk gskfkgtdys vgedfsrrvq ncrkhlvafa raksvpfslr
      361 fktlhigpkr yvfdelsesv reip
//
LOCUS       XP_077504950             229 aa            linear   INV 25-NOV-2025
DEFINITION  PR domain zinc finger protein 14-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504950
VERSION     XP_077504950.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648824.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..229
                     /product="PR domain zinc finger protein 14-like isoform
                     X1"
                     /calculated_mol_wt=25250
     Region          <1..72
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(22..27,38..40,61..64)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Region          100..120
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(100,103,116,120)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          129..153
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          131..151
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(131,134,147,151)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(136,138,140,142..143,146..147,150,164,166,170..171,
                     174..175,178,192,194,196,198..199)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          143..168
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          159..179
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          171..196
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     CDS             1..229
                     /gene="LOC144114836"
                     /coded_by="XM_077648824.1:326..1015"
                     /db_xref="GeneID:144114836"
ORIGIN      
        1 mvfkdgevfl vdgrpldrsn wmryvncaas pqeqnlvafr rygnkyyrtp kavgageell
       61 vwygaafare lgllgkprgs gpsakeggns evrpkcvfsc gacgelfsaq dllekhrrrk
      121 hpqrpegrhr cahcpyssdr kpnvvrhvrt htgwrpflcq vcgkgfarld hltghqlvhs
      181 gegphecpec gqrfsqasnq tgcspqgaal cgrrgclapv splrevvqp
//
LOCUS       XP_077504951             208 aa            linear   INV 25-NOV-2025
DEFINITION  PR domain zinc finger protein 14-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504951
VERSION     XP_077504951.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648825.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..208
                     /product="PR domain zinc finger protein 14-like isoform
                     X2"
                     /calculated_mol_wt=22787
     Region          <1..51
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(1..6,17..19,40..43)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Region          79..99
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(79,82,95,99)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          108..132
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          110..130
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(110,113,126,130)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(115,117,119,121..122,125..126,129,143,145,149..150,
                     153..154,157,171,173,175,177..178)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          122..147
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          138..158
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          150..175
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     CDS             1..208
                     /gene="LOC144114836"
                     /coded_by="XM_077648825.1:183..809"
                     /db_xref="GeneID:144114836"
ORIGIN      
        1 mryvncaasp qeqnlvafrr ygnkyyrtpk avgageellv wygaafarel gllgkprgsg
       61 psakeggnse vrpkcvfscg acgelfsaqd llekhrrrkh pqrpegrhrc ahcpyssdrk
      121 pnvvrhvrth tgwrpflcqv cgkgfarldh ltghqlvhsg egphecpecg qrfsqasnqt
      181 gcspqgaalc grrgclapvs plrevvqp
//
LOCUS       XP_077504952             229 aa            linear   INV 25-NOV-2025
DEFINITION  PR domain zinc finger protein 14-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504952
VERSION     XP_077504952.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648826.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..229
                     /product="PR domain zinc finger protein 14-like isoform
                     X1"
                     /calculated_mol_wt=25232
     Region          <1..72
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(22..27,38..40,61..64)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Region          100..121
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(100,103,116,121)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          129..153
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          131..151
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(131,134,147,151)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(136,138,140,142..143,146..147,150,164,166,170..171,
                     174..175,178,192,194,196,198..199)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          143..168
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          159..179
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..229
                     /gene="LOC144114838"
                     /coded_by="XM_077648826.1:550..1239"
                     /db_xref="GeneID:144114838"
ORIGIN      
        1 mvfkdgevfl vdgrpldrsn wmryvncaas pqeqnmvafr rygnkyyrtp kavgageell
       61 vwcgaafare lgllgkprgs gpsakeggns evrskcvfsc gacgelfsaq dllekhrrrk
      121 hpqrlegrhr cahcpyssdq kpnvvrhvrt htgwrpflcq vcgkgfarld hltghqlvhs
      181 gespheclec gqrfsqasnq tgcspqgaal cgrrgclapv splrevvqp
//
LOCUS       XP_077504953             208 aa            linear   INV 25-NOV-2025
DEFINITION  PR domain zinc finger protein 14-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504953
VERSION     XP_077504953.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648827.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..208
                     /product="PR domain zinc finger protein 14-like isoform
                     X2"
                     /calculated_mol_wt=22769
     Region          <1..51
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(1..6,17..19,40..43)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Region          79..100
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(79,82,95,100)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          108..132
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          110..130
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(110,113,126,130)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(115,117,119,121..122,125..126,129,143,145,149..150,
                     153..154,157,171,173,175,177..178)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          122..147
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          138..158
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..208
                     /gene="LOC144114838"
                     /coded_by="XM_077648827.1:1997..2623"
                     /db_xref="GeneID:144114838"
ORIGIN      
        1 mryvncaasp qeqnmvafrr ygnkyyrtpk avgageellv wcgaafarel gllgkprgsg
       61 psakeggnse vrskcvfscg acgelfsaqd llekhrrrkh pqrlegrhrc ahcpyssdqk
      121 pnvvrhvrth tgwrpflcqv cgkgfarldh ltghqlvhsg espheclecg qrfsqasnqt
      181 gcspqgaalc grrgclapvs plrevvqp
//
LOCUS       XP_077504954             384 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114839 [Amblyomma americanum].
ACCESSION   XP_077504954
VERSION     XP_077504954.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648828.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..384
                     /product="uncharacterized protein LOC144114839"
                     /calculated_mol_wt=42699
     CDS             1..384
                     /gene="LOC144114839"
                     /coded_by="XM_077648828.1:1..1155"
                     /db_xref="GeneID:144114839"
ORIGIN      
        1 mqvhspsiiw lllnavfcat wataierapl rgaadgrtlc aattnppiqv nltlradwea
       61 pssattltvp sikeqrpsta pcglggcatt rlhnpllfam qvgnhrtift kktsnyffvq
      121 lpspqcclat ciecfhvvrs lllmcgdies npgpekldvi lgelkkmstg qtelvkevqe
      181 lkgqllsidl kmtnlttrls aletdyesls tlrndlegiq atsaatsrlv gvlearldda
      241 enrsrrnnll fyglpdtnpt etysltedli irhcsdhldt qlspndiera hrlgrhskdr
      301 krpiivkfts fktkesilsk gskfkgtdys vgedfsrrvq ncrkhlvafa raksvpfslr
      361 fktlhigpkr yvfdelsesv reip
//
LOCUS       XP_077504955             260 aa            linear   INV 25-NOV-2025
DEFINITION  PR domain zinc finger protein 14-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504955
VERSION     XP_077504955.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648829.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..260
                     /product="PR domain zinc finger protein 14-like isoform
                     X1"
                     /calculated_mol_wt=28427
     Region          <13..74
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(24..29,40..42,63..66)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Region          102..123
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(102,105,118,123)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          133..153
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(133,136,149,153)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(138,140,142,144..145,148..149,152,166,168,172..173,
                     176..177,180,194,196,198,200)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          145..170
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          161..181
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          173..198
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     CDS             1..260
                     /gene="LOC144114840"
                     /coded_by="XM_077648829.1:29..811"
                     /db_xref="GeneID:144114840"
ORIGIN      
        1 mdavfkdgev flvdgrpldr snwmryvnca aspqeqnlva frrygnkyyr tpkavgagee
       61 llvwygaafa relgllgkpr gsgpsakegg nsevrskcvf scgacgelfs aqdllekhrr
      121 rkhpqrlegr hrcahcpyss drkpnvvrhv rthtswrpfl cqvcgkgfar ldhltghqlv
      181 hsgegphecp ecgqrfsqas yqtgcspqga alcgrrglpr tcvpaagsgs tiratstpad
      241 khggeaprll tvrqevhatc
//
LOCUS       XP_077504956             237 aa            linear   INV 25-NOV-2025
DEFINITION  PR domain zinc finger protein 14-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504956
VERSION     XP_077504956.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648830.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..237
                     /product="PR domain zinc finger protein 14-like isoform
                     X2"
                     /calculated_mol_wt=25778
     Region          <1..51
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(1..6,17..19,40..43)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Region          79..100
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(79,82,95,100)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          110..130
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(110,113,126,130)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(115,117,119,121..122,125..126,129,143,145,149..150,
                     153..154,157,171,173,175,177)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          122..147
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          138..158
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          150..175
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     CDS             1..237
                     /gene="LOC144114840"
                     /coded_by="XM_077648830.1:774..1487"
                     /db_xref="GeneID:144114840"
ORIGIN      
        1 mryvncaasp qeqnlvafrr ygnkyyrtpk avgageellv wygaafarel gllgkprgsg
       61 psakeggnse vrskcvfscg acgelfsaqd llekhrrrkh pqrlegrhrc ahcpyssdrk
      121 pnvvrhvrth tswrpflcqv cgkgfarldh ltghqlvhsg egphecpecg qrfsqasyqt
      181 gcspqgaalc grrglprtcv paagsgstir atstpadkhg geaprlltvr qevhatc
//
LOCUS       XP_077504957             237 aa            linear   INV 25-NOV-2025
DEFINITION  PR domain zinc finger protein 14-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504957
VERSION     XP_077504957.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648831.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..237
                     /product="PR domain zinc finger protein 14-like isoform
                     X2"
                     /calculated_mol_wt=25778
     Region          <1..51
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(1..6,17..19,40..43)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Region          79..100
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(79,82,95,100)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          110..130
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(110,113,126,130)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(115,117,119,121..122,125..126,129,143,145,149..150,
                     153..154,157,171,173,175,177)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          122..147
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          138..158
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          150..175
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     CDS             1..237
                     /gene="LOC144114840"
                     /coded_by="XM_077648831.1:286..999"
                     /db_xref="GeneID:144114840"
ORIGIN      
        1 mryvncaasp qeqnlvafrr ygnkyyrtpk avgageellv wygaafarel gllgkprgsg
       61 psakeggnse vrskcvfscg acgelfsaqd llekhrrrkh pqrlegrhrc ahcpyssdrk
      121 pnvvrhvrth tswrpflcqv cgkgfarldh ltghqlvhsg egphecpecg qrfsqasyqt
      181 gcspqgaalc grrglprtcv paagsgstir atstpadkhg geaprlltvr qevhatc
//
LOCUS       XP_077504958             209 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114842 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504958
VERSION     XP_077504958.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648832.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..209
                     /product="uncharacterized protein LOC144114842 isoform X1"
                     /calculated_mol_wt=22679
     Region          <78..>139
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          80..100
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(80,83,96,100)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          111..133
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          111..131
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(111,114,127,131)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(116,118,120,122..123,126..127,130,144,146,150..151,
                     154..155,158,172,174,176,178..179)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          123..148
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          139..159
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..209
                     /gene="LOC144114842"
                     /coded_by="XM_077648832.1:214..843"
                     /db_xref="GeneID:144114842"
ORIGIN      
        1 mdpgvffgkr pfcrlseale vaadvavhae epvdsvivpp epsvdtdvee gdddgttdtv
       61 vndvseggns evrpkcvfsc gacgelfsaq dllekhrrrk hpqrpegrhc cahcpyssdr
      121 kpnvvrhvrt htgwrpflcq vcgkgfarld hltghqlvhs geglhecpec gqrfsqasnq
      181 tgcspqgaal cgrrgclapv yplrevvqp
//
LOCUS       XP_077504959             208 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114842 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504959
VERSION     XP_077504959.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648833.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..208
                     /product="uncharacterized protein LOC144114842 isoform X2"
                     /calculated_mol_wt=22549
     Region          <77..>138
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          79..99
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(79,82,95,99)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          110..132
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          110..130
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(110,113,126,130)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(115,117,119,121..122,125..126,129,143,145,149..150,
                     153..154,157,171,173,175,177..178)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          122..147
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          138..158
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..208
                     /gene="LOC144114842"
                     /coded_by="XM_077648833.1:214..840"
                     /db_xref="GeneID:144114842"
ORIGIN      
        1 mdpgvffgkr pfcrlseale vaadvavhae epvdsvivpp epsvdtdvee gdddgttdtv
       61 vndvsggnse vrpkcvfscg acgelfsaqd llekhrrrkh pqrpegrhcc ahcpyssdrk
      121 pnvvrhvrth tgwrpflcqv cgkgfarldh ltghqlvhsg eglhecpecg qrfsqasnqt
      181 gcspqgaalc grrgclapvy plrevvqp
//
LOCUS       XP_077504961             384 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114843 [Amblyomma americanum].
ACCESSION   XP_077504961
VERSION     XP_077504961.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648835.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..384
                     /product="uncharacterized protein LOC144114843"
                     /calculated_mol_wt=42656
     CDS             1..384
                     /gene="LOC144114843"
                     /coded_by="XM_077648835.1:1..1155"
                     /db_xref="GeneID:144114843"
ORIGIN      
        1 mqvhspsiiw lllnavfcat wataierapl rgaadgrtlc aattnppiqv nltlsadwea
       61 pssattltvp fikeqrpsta pcglggcatt rlhnpllfam qvgnhrtift kktsnyflvq
      121 lpspqcclat ciecfhvvrs lllmcgdies npgpekldvi lgelkkmstg qtelvkevqe
      181 lkgqllsidl kmtnlttrls aletdyesls tlrndlegiq atsaatsrlv gvlearldda
      241 enrsrrnnll fyglpdtnpt etysltedli irhcsdhldt qlspndiera hrlgrhskdr
      301 krpiivkfts fktkesilsk gskfkgtdys vgedfsrrvq ncrkhlvafa raksvpfslr
      361 fktlhigpkr yvfdelsesv reip
//
LOCUS       XP_077504962             231 aa            linear   INV 25-NOV-2025
DEFINITION  PR domain zinc finger protein 14-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504962
VERSION     XP_077504962.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648836.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..231
                     /product="PR domain zinc finger protein 14-like isoform
                     X1"
                     /calculated_mol_wt=25429
     Region          <13..74
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(24..29,40..42,63..66)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Region          102..122
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(102,105,118,122)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          133..153
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(133,136,149,153)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(138,140,142,144..145,148..149,152,166,168,172..173,
                     176..177,180,194,196,198,200..201)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          161..181
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..231
                     /gene="LOC144114844"
                     /coded_by="XM_077648836.1:26..721"
                     /db_xref="GeneID:144114844"
ORIGIN      
        1 mlavfkdgev flvdgrpldr snwmryvnca aspqeqnmva frrygnkyyr tpkavgagee
       61 llvwcgaafa relgllgkpr gsgpsakegg nsevrpkcvf scgacgelfs aqdllekhrr
      121 rkhpqrpegr hrcahcpyss drkpnvvrhv wtqtgwrpfl cqvcgkgfar ldhltghqlv
      181 hsgeglhecp ecgqrfsqas nqtgcspqga alcgrrgcla pvsplrevvq p
//
LOCUS       XP_077504963             229 aa            linear   INV 25-NOV-2025
DEFINITION  PR domain zinc finger protein 14-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504963
VERSION     XP_077504963.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648837.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..229
                     /product="PR domain zinc finger protein 14-like isoform
                     X2"
                     /calculated_mol_wt=25245
     Region          <1..72
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(22..27,38..40,61..64)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Region          100..120
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(100,103,116,120)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          131..151
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(131,134,147,151)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(136,138,140,142..143,146..147,150,164,166,170..171,
                     174..175,178,192,194,196,198..199)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          159..179
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..229
                     /gene="LOC144114844"
                     /coded_by="XM_077648837.1:726..1415"
                     /db_xref="GeneID:144114844"
ORIGIN      
        1 mvfkdgevfl vdgrpldrsn wmryvncaas pqeqnmvafr rygnkyyrtp kavgageell
       61 vwcgaafare lgllgkprgs gpsakeggns evrpkcvfsc gacgelfsaq dllekhrrrk
      121 hpqrpegrhr cahcpyssdr kpnvvrhvwt qtgwrpflcq vcgkgfarld hltghqlvhs
      181 geglhecpec gqrfsqasnq tgcspqgaal cgrrgclapv splrevvqp
//
LOCUS       XP_077504964             208 aa            linear   INV 25-NOV-2025
DEFINITION  PR domain zinc finger protein 14-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504964
VERSION     XP_077504964.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648838.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..208
                     /product="PR domain zinc finger protein 14-like isoform
                     X3"
                     /calculated_mol_wt=22782
     Region          <1..51
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(1..6,17..19,40..43)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Region          79..99
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(79,82,95,99)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          110..130
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(110,113,126,130)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(115,117,119,121..122,125..126,129,143,145,149..150,
                     153..154,157,171,173,175,177..178)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          138..158
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..208
                     /gene="LOC144114844"
                     /coded_by="XM_077648838.1:606..1232"
                     /db_xref="GeneID:144114844"
ORIGIN      
        1 mryvncaasp qeqnmvafrr ygnkyyrtpk avgageellv wcgaafarel gllgkprgsg
       61 psakeggnse vrpkcvfscg acgelfsaqd llekhrrrkh pqrpegrhrc ahcpyssdrk
      121 pnvvrhvwtq tgwrpflcqv cgkgfarldh ltghqlvhsg eglhecpecg qrfsqasnqt
      181 gcspqgaalc grrgclapvs plrevvqp
//
LOCUS       XP_077504965             208 aa            linear   INV 25-NOV-2025
DEFINITION  PR domain zinc finger protein 14-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504965
VERSION     XP_077504965.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648839.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..208
                     /product="PR domain zinc finger protein 14-like isoform
                     X3"
                     /calculated_mol_wt=22782
     Region          <1..51
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(1..6,17..19,40..43)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Region          79..99
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(79,82,95,99)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          110..130
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(110,113,126,130)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(115,117,119,121..122,125..126,129,143,145,149..150,
                     153..154,157,171,173,175,177..178)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          138..158
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..208
                     /gene="LOC144114844"
                     /coded_by="XM_077648839.1:1531..2157"
                     /db_xref="GeneID:144114844"
ORIGIN      
        1 mryvncaasp qeqnmvafrr ygnkyyrtpk avgageellv wcgaafarel gllgkprgsg
       61 psakeggnse vrpkcvfscg acgelfsaqd llekhrrrkh pqrpegrhrc ahcpyssdrk
      121 pnvvrhvwtq tgwrpflcqv cgkgfarldh ltghqlvhsg eglhecpecg qrfsqasnqt
      181 gcspqgaalc grrgclapvs plrevvqp
//
LOCUS       XP_077504966             209 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114845 [Amblyomma americanum].
ACCESSION   XP_077504966
VERSION     XP_077504966.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648840.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..209
                     /product="uncharacterized protein LOC144114845"
                     /calculated_mol_wt=22705
     Region          <78..>139
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          80..100
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(80,83,96,100)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          111..133
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          111..131
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(111,114,127,131)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(116,118,120,122..123,126..127,130,144,146,150..151,
                     154..155,158,172,174,176,178..179)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          123..148
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          139..159
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..209
                     /gene="LOC144114845"
                     /coded_by="XM_077648840.1:46..675"
                     /db_xref="GeneID:144114845"
ORIGIN      
        1 mdpgvffgkr pfcrlseale vaadvavhae epvdivivpp epsvdtdvee adddgttdtv
       61 vndvseggns evrpkcvfsc gacgelfsaq dllekhrrrk hpqrpegrhc cahcpyssdr
      121 kpnvvrhvrt htgwrpflcq vcgkgfarld hltghqlvhs geglhecpdc gqrfsqasnq
      181 tgcspqgaal cgrrgclapv yplrevvqp
//
LOCUS       XP_077504967             188 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114846 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504967
VERSION     XP_077504967.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648841.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..188
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..188
                     /product="uncharacterized protein LOC144114846 isoform X1"
                     /calculated_mol_wt=20090
     CDS             1..188
                     /gene="LOC144114846"
                     /coded_by="XM_077648841.1:1233..1799"
                     /db_xref="GeneID:144114846"
ORIGIN      
        1 mavvtgppev pnslpketsv vplqapllcg cccfpgsais spsgaaarss fppaswpcla
       61 dtsfytwqrg ssassdigda ykskpgtthl ghstvtamei lplrilqtil ltftasrlyv
      121 rylalhpaaf salahgplpq grqkaplkph deghfsyhek lennshcsmy saalfdtsrr
      181 ksqffvls
//
LOCUS       XP_077504968             188 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114846 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504968
VERSION     XP_077504968.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648842.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..188
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..188
                     /product="uncharacterized protein LOC144114846 isoform X1"
                     /calculated_mol_wt=20090
     CDS             1..188
                     /gene="LOC144114846"
                     /coded_by="XM_077648842.1:514..1080"
                     /db_xref="GeneID:144114846"
ORIGIN      
        1 mavvtgppev pnslpketsv vplqapllcg cccfpgsais spsgaaarss fppaswpcla
       61 dtsfytwqrg ssassdigda ykskpgtthl ghstvtamei lplrilqtil ltftasrlyv
      121 rylalhpaaf salahgplpq grqkaplkph deghfsyhek lennshcsmy saalfdtsrr
      181 ksqffvls
//
LOCUS       XP_077504969             188 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114846 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504969
VERSION     XP_077504969.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648843.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..188
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..188
                     /product="uncharacterized protein LOC144114846 isoform X1"
                     /calculated_mol_wt=20090
     CDS             1..188
                     /gene="LOC144114846"
                     /coded_by="XM_077648843.1:519..1085"
                     /db_xref="GeneID:144114846"
ORIGIN      
        1 mavvtgppev pnslpketsv vplqapllcg cccfpgsais spsgaaarss fppaswpcla
       61 dtsfytwqrg ssassdigda ykskpgtthl ghstvtamei lplrilqtil ltftasrlyv
      121 rylalhpaaf salahgplpq grqkaplkph deghfsyhek lennshcsmy saalfdtsrr
      181 ksqffvls
//
LOCUS       XP_077504970             188 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114846 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504970
VERSION     XP_077504970.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648844.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..188
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..188
                     /product="uncharacterized protein LOC144114846 isoform X1"
                     /calculated_mol_wt=20090
     CDS             1..188
                     /gene="LOC144114846"
                     /coded_by="XM_077648844.1:427..993"
                     /db_xref="GeneID:144114846"
ORIGIN      
        1 mavvtgppev pnslpketsv vplqapllcg cccfpgsais spsgaaarss fppaswpcla
       61 dtsfytwqrg ssassdigda ykskpgtthl ghstvtamei lplrilqtil ltftasrlyv
      121 rylalhpaaf salahgplpq grqkaplkph deghfsyhek lennshcsmy saalfdtsrr
      181 ksqffvls
//
LOCUS       XP_077504971             179 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114846 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504971
VERSION     XP_077504971.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648845.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..179
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..179
                     /product="uncharacterized protein LOC144114846 isoform X2"
                     /calculated_mol_wt=18958
     CDS             1..179
                     /gene="LOC144114846"
                     /coded_by="XM_077648845.1:692..1231"
                     /db_xref="GeneID:144114846"
ORIGIN      
        1 mavvtgppev pnslpketsv vplqapllcg cccfpgsais spsgaaarss fppaswpcla
       61 dtsfytwqrg ssassdigda ykskpgtthl ghstvtamei lplrilqtil ltftasrlyv
      121 rylalhpaaf salahgplpq grqkaplkph deghfsyhek lennshcngl hingpqtmq
//
LOCUS       XP_077504972             139 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114846 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504972
VERSION     XP_077504972.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648846.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..139
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..139
                     /product="uncharacterized protein LOC144114846 isoform X3"
                     /calculated_mol_wt=14556
     CDS             1..139
                     /gene="LOC144114846"
                     /coded_by="XM_077648846.1:692..1111"
                     /db_xref="GeneID:144114846"
ORIGIN      
        1 mavvtgppev pnslpketsv vplqapllcg cccfpgsais spsgaaarss fppaswpcla
       61 dtsfytwqrg ssassdigda ykskpgtthl ghstvtamei lplrilqtil ltftasrlyv
      121 rylalhpdgl hingpqtmq
//
LOCUS       XP_077504974             122 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114849 [Amblyomma americanum].
ACCESSION   XP_077504974
VERSION     XP_077504974.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648848.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..122
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..122
                     /product="uncharacterized protein LOC144114849"
                     /calculated_mol_wt=12585
     CDS             1..122
                     /gene="LOC144114849"
                     /coded_by="XM_077648848.1:483..851"
                     /db_xref="GeneID:144114849"
ORIGIN      
        1 mavvtgppev pnslpketsv vplqapllcg cccfpgsais spsgaaarss fppaswpcld
       61 dtsfytwqrg ssassdigda ykskpgtthl ghstvtamei lplrilqnet tssrpplsht
      121 ls
//
LOCUS       XP_077504975             139 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114850 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504975
VERSION     XP_077504975.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648849.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..139
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..139
                     /product="uncharacterized protein LOC144114850 isoform X1"
                     /calculated_mol_wt=14556
     CDS             1..139
                     /gene="LOC144114850"
                     /coded_by="XM_077648849.1:894..1313"
                     /db_xref="GeneID:144114850"
ORIGIN      
        1 mavvtgppev pnslpketsv vplqapllcg cccfpgsais spsgaaarss fppaswpcla
       61 dtsfytwqrg ssassdigda ykskpgtthl ghstvtamei lplrilqtil ltftasrlyv
      121 rylalhpdgl hingpqtmq
//
LOCUS       XP_077504976             122 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114850 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504976
VERSION     XP_077504976.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648850.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..122
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..122
                     /product="uncharacterized protein LOC144114850 isoform X2"
                     /calculated_mol_wt=12541
     CDS             1..122
                     /gene="LOC144114850"
                     /coded_by="XM_077648850.1:894..1262"
                     /db_xref="GeneID:144114850"
ORIGIN      
        1 mavvtgppev pnslpketsv vplqapllcg cccfpgsais spsgaaarss fppaswpcla
       61 dtsfytwqrg ssassdigda ykskpgtthl ghstvtamei lplrilqnet tssrpplsht
      121 ls
//
LOCUS       XP_077504978             139 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114853 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504978
VERSION     XP_077504978.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648852.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..139
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..139
                     /product="uncharacterized protein LOC144114853 isoform X1"
                     /calculated_mol_wt=14556
     CDS             1..139
                     /gene="LOC144114853"
                     /coded_by="XM_077648852.1:456..875"
                     /db_xref="GeneID:144114853"
ORIGIN      
        1 mavvtgppev pnslpketsv vplqapllcg cccfpgsais spsgaaarss fppaswpcla
       61 dtsfytwqrg ssassdigda ykskpgtthl ghstvtamei lplrilqtil ltftasrlyv
      121 rylalhpdgl hingpqtmq
//
LOCUS       XP_077504979             122 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114853 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504979
VERSION     XP_077504979.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648853.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..122
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..122
                     /product="uncharacterized protein LOC144114853 isoform X2"
                     /calculated_mol_wt=12541
     CDS             1..122
                     /gene="LOC144114853"
                     /coded_by="XM_077648853.1:456..824"
                     /db_xref="GeneID:144114853"
ORIGIN      
        1 mavvtgppev pnslpketsv vplqapllcg cccfpgsais spsgaaarss fppaswpcla
       61 dtsfytwqrg ssassdigda ykskpgtthl ghstvtamei lplrilqnet tssrpplsht
      121 ls
//
LOCUS       XP_077504980             137 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114854 [Amblyomma americanum].
ACCESSION   XP_077504980
VERSION     XP_077504980.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648854.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..137
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..137
                     /product="uncharacterized protein LOC144114854"
                     /calculated_mol_wt=14886
     Region          <19..76
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..137
                     /gene="LOC144114854"
                     /coded_by="XM_077648854.1:150..563"
                     /db_xref="GeneID:144114854"
ORIGIN      
        1 msastarvvq mrkstetaka ispsmcryqq ahiktgnvve rtfgvwkrlf pcldmglqhk
       61 pkqaaviita caalqnfacl mkepqppies apiqdaplar tsttaarrpe hlppvdavsd
      121 slsgmqarrv liqksft
//
LOCUS       XP_077504981            1103 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114855 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504981
VERSION     XP_077504981.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648855.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1103
                     /product="uncharacterized protein LOC144114855 isoform X1"
                     /calculated_mol_wt=120296
     Region          804..958
                     /region_name="Anillin"
                     /note="Cell division protein anillin; pfam08174"
                     /db_xref="CDD:462393"
     Region          990..1095
                     /region_name="PH_anillin"
                     /note="Anillin Pleckstrin homology (PH) domain; cd01263"
                     /db_xref="CDD:269964"
     CDS             1..1103
                     /gene="LOC144114855"
                     /coded_by="XM_077648855.1:100..3411"
                     /db_xref="GeneID:144114855"
ORIGIN      
        1 mddideyarr laertrarsk nfhhktaepp pakrsltadd lncgsdgatg kkasppkrqc
       61 lgdekenvaa hspglstsrr stesvsalgp knshdlegva aprraviaeg sgvprglaal
      121 rrrrdvspds gckesqqalr qaalaaddgf trtprglaal rrrqqvspep davpssmcvs
      181 kpsspakves kpkqlpgsre agagsslrts erknnwdpkv iasleaqgft kspsasklsy
      241 sfksadchev paspariptr eemhegpsti pappplpppm pspkasgsva akvdnakktv
      301 aekvavsnav tihrqplgds cqggvpqrqr aelhgrdpse lslqqrralf eaavvaeeee
      361 qpcaapeklt vaqrkalfeq qmklskaaae rsypvvkqgk plkkpvgnea kssvaqiyce
      421 kvtvenqeqn fgvaerrarl egmaqnkelt peyhsfknvt lrsavnhevc hgqsslhssn
      481 tikgftqthv siidapvtdf agsphsstse efegksslgn dsaseddsdp vtsggsptav
      541 qasiftsape deenmdesgl grsweqtsge aegaigkrfn essdlatydq qyahmkalee
      601 eiarekavlt edriecteer shreadtksv ssdtehiega lkkevlrnrl ypelppmewn
      661 dvpvavpppk prraaasped gqplvhtqsv yrrkvctplr qleqsatpgh tqftpaaqts
      721 nqmdiqlqik alykeaeaqe vviaqasral evcrlttefl gsaeqveger llllaterrq
      781 aclaeverlk trggcgddev vperasltls qlqlplkref laaqlegilg edvhyflcli
      841 shgaqvlasq mlstadnagt nalcfsnhmt lrdlradfav kvhvyalqtk ketlphhrky
      901 riggkdgkhk ltpkgsskgs vsksalspat vcapsgsalr tpsfglvgsl qltvancrrs
      961 kfnlekvpph splegtllmq ltlrpehhhe lrgflsmfee vggfgawhrr wcvlaenrls
     1021 ywrypedess kepvgrlelt qctspearla srdicarpht lilhingkdy llsadtkeer
     1081 qnwcsnlttv lkslrtwagm vpn
//
LOCUS       XP_077504982            1098 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114855 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504982
VERSION     XP_077504982.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648856.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1098
                     /product="uncharacterized protein LOC144114855 isoform X2"
                     /calculated_mol_wt=119812
     Region          799..953
                     /region_name="Anillin"
                     /note="Cell division protein anillin; pfam08174"
                     /db_xref="CDD:462393"
     Region          985..1090
                     /region_name="PH_anillin"
                     /note="Anillin Pleckstrin homology (PH) domain; cd01263"
                     /db_xref="CDD:269964"
     CDS             1..1098
                     /gene="LOC144114855"
                     /coded_by="XM_077648856.1:150..3446"
                     /db_xref="GeneID:144114855"
ORIGIN      
        1 mddideyarr laertrarsk nfhhktaepp pakrsltadd lncgsdgatg kkasppkrqc
       61 lgdekenvaa hspglstsrr stesvsalgp knshdlegva aprraviaeg sgvprglaal
      121 rrrrdvspds gckesqqalr qaalaaddgf trtprglaal rrrqqvspep davpssmcvs
      181 kpsspakves kpkqlpgsre agagsslrts erknnwdpke aqgftkspsa sklsysfksa
      241 dchevpaspa riptreemhe gpstipappp lpppmpspka sgsvaakvdn akktvaekva
      301 vsnavtihrq plgdscqggv pqrqraelhg rdpselslqq rralfeaavv aeeeeqpcaa
      361 pekltvaqrk alfeqqmkls kaaaersypv vkqgkplkkp vgneakssva qiycekvtve
      421 nqeqnfgvae rrarlegmaq nkeltpeyhs fknvtlrsav nhevchgqss lhssntikgf
      481 tqthvsiida pvtdfagsph sstseefegk sslgndsase ddsdpvtsgg sptavqasif
      541 tsapedeenm desglgrswe qtsgeaegai gkrfnessdl atydqqyahm kaleeeiare
      601 kavltedrie cteershrea dtksvssdte hiegalkkev lrnrlypelp pmewndvpva
      661 vpppkprraa aspedgqplv htqsvyrrkv ctplrqleqs atpghtqftp aaqtsnqmdi
      721 qlqikalyke aeaqevviaq asralevcrl tteflgsaeq vegerlllla terrqaclae
      781 verlktrggc gddevvpera sltlsqlqlp lkreflaaql egilgedvhy flclishgaq
      841 vlasqmlsta dnagtnalcf snhmtlrdlr adfavkvhvy alqtkketlp hhrkyriggk
      901 dgkhkltpkg sskgsvsksa lspatvcaps gsalrtpsfg lvgslqltva ncrrskfnle
      961 kvpphspleg tllmqltlrp ehhhelrgfl smfeevggfg awhrrwcvla enrlsywryp
     1021 edesskepvg rleltqctsp earlasrdic arphtlilhi ngkdyllsad tkeerqnwcs
     1081 nlttvlkslr twagmvpn
//
LOCUS       XP_077504983            1801 aa            linear   INV 25-NOV-2025
DEFINITION  putative vitellogenin receptor yl [Amblyomma americanum].
ACCESSION   XP_077504983
VERSION     XP_077504983.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648857.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1801
                     /product="putative vitellogenin receptor yl"
                     /calculated_mol_wt=198831
     Region          24..58
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(29,37,48..49)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(41,44,48,54..55)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            51..55
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          65..100
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(70,79,90..91)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(83,86,90,96..97)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            93..97
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          108..137
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(108,116,127..128)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(120,123,127,133..134)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            130..134
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          152..186
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(160,168,179..180)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(172,175,179,185..186)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            182..186
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          212..233
                     /region_name="cEGF"
                     /note="Complement Clr-like EGF-like; pfam12662"
                     /db_xref="CDD:463661"
     Region          <228..266
                     /region_name="vWFA"
                     /note="Von Willebrand factor type A (vWA) domain was
                     originally found in the blood coagulation protein von
                     Willebrand factor (vWF). Typically, the vWA domain is made
                     up of approximately 200 amino acid residues folded into a
                     classic a/b para-rossmann type of...; cl00057"
                     /db_xref="CDD:469594"
     Region          <360..509
                     /region_name="YncE"
                     /note="DNA-binding beta-propeller fold protein YncE
                     [General function prediction only]; COG3391"
                     /db_xref="CDD:442618"
     Region          404..444
                     /region_name="Ldl_recept_b"
                     /note="Low-density lipoprotein receptor repeat class B;
                     pfam00058"
                     /db_xref="CDD:459654"
     Region          427..469
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          546..574
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     Region          607..643
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          650..689
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          735..770
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          851..887
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     Region          894..927
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(899,906,917..918)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(910,913,917,923..924)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            920..924
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          931..965
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(936,944,955..956)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(948,951,955,961..962)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            958..962
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          970..1004
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(975,983,994..995)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(987,990,994,1000..1001)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            997..1001
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1009..1043
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1014,1022,1033..1034)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1026,1029,1033,1039..1040)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1036..1040
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1049..1083
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1054,1062,1073..1074)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1066,1069,1073,1079..1080)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1076..1080
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1093..1127
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1098,1106,1117..1118)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1110,1113,1117,1123..1124)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1120..1124
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1182..1213
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1187,1195,1206..1207)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1199,1202,1206,1212..1213)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1209..1213
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1221..1256
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     Region          1258..1292
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain; smart00179"
                     /db_xref="CDD:214542"
     Site            order(1258,1261,1274)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          1428..1473
                     /region_name="Ldl_recept_b"
                     /note="Low-density lipoprotein receptor repeat class B;
                     pfam00058"
                     /db_xref="CDD:459654"
     Region          1457..1499
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          1505..1541
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          1574..1602
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     CDS             1..1801
                     /gene="yl"
                     /coded_by="XM_077648857.1:90..5495"
                     /db_xref="GeneID:144114857"
ORIGIN      
        1 maavtralla vlllqiahqa ssecargwfd cgngrciamf wrcdgqndcg nhkdesgctn
       61 ahrtcpvekf acqdssycvp eiwvcdgead chdssdeldc hssncsghrc hnnecipshw
      121 rcdgtedcad asdelgcaat gaasttttvs prcdvdqgrf pcldgqcllp gkvcdgkkdc
      181 tdgadegtfc kvnecsqkkc sqgcfvatng stcycnpgfr lmadhtscad idecaehphl
      241 cshgctnspg sykcscvegy qltdnsfcka rdpeplllfs ttneirglwi rsnryfevhp
      301 aeaqavgvef dseqlrlfwt dvstkqssvy scrldgsgfk tlftaertll edlsldwvtn
      361 nlyitdslgk rilvcstdgl scgavvttel dspraiivnp tqklmywtdw gvkpsitrsg
      421 mdgssiqrlv ttalgwpngl aldhptnrly wcdaklsrle yldlttmert vvleeslfhp
      481 falsvfedtv ywsdwasysl dtsnkltgkq hhrllrengr himgvhvyhp ilrqrgisnp
      541 cwdnpcdhic vlsgdsymcl crlgyklssn khsctvtkdf sfaivaeedc lykidlrtvg
      601 apfptkvpls dvgmiaalaf dwsnqtlyfs dnqreilaai nmntfeqrav ydhigsvfgi
      661 dydmthglly wvdadkytle vcrangsgha ivrddlrrpv gialypfagv lfvltagdrp
      721 aitcytmdgq npkplpltsl lvpvsiavdl varklvwada trgtiesldl vtvftgtpfi
      781 vhqvkshvss vsaahneihw tsrdnssley idlsaephvr rsvplrtgrn gtysrrvlaa
      841 sqvpdfepgl carsnggcsh tclpvrtter acfcppgmal rtdnttcrve ngtcrphelp
      901 caggciaaay wcdghkdcad nadeatcgtt cpskdfacqn gkcvemawrc dgyddcddqs
      961 deancphqtc ashqftcksg aclpfywrcd gandcpdgdd eldcrtlrcp nghdrcangq
     1021 ciprdwscdg hadcsdssde tnctetvscf eddfhcangq cidkrlrcdh dedcednsde
     1081 sgcdyapann skcvkgmvgc gdgqcvythd mcdgyadchn gwdehncsap vcqsaeffcp
     1141 gtkrcilqsw lcdgdddcgd amdellarcr patippstna pcwsdqfqcg sheciawssv
     1201 cdgrtdcadf sdegshcehh catanggcah icrespsgpl cscrpgyrln sdrkscddvd
     1261 eclspghcsh fcqnskggyk ctcaagyalg adrryckvqy geafllymlp nqirsfsmhg
     1321 hvqhvlaedt lsdmhgmdyr isdksifwte meegvinvvt lgnskqftll ddihrpfhia
     1381 ldwvagniyf tdgwvhiqac estfkhctdv vdtayshvnt falaandglm fwgvwheivn
     1441 kdygvieran mdgtdrrill tdkilwpcsi tvdavhlriy wsdanknvie satyqgkdrk
     1501 lvrgtglasp fsialfedwl ywsdwgsdsl macnkytgrn valvhhgmsk asvlkvlhav
     1561 hqpsgvnrca rnscghicll kpssykcacg hgftlakdsq ncvesdersy nltsadvlgq
     1621 lcnpvclngg hcisekssyy crctkdfqgp scqdpvvvym aphrassrst wlapilitll
     1681 cisllvlgyi lyqrnknkla aldfsvsfkk ptfgkregll qhehpvgade dyhamttqep
     1741 gfgnpvfapp ksqllledgq fkrwassdsl qssdsqekst iafagqfssa kqdkvfflrk
     1801 s
//
LOCUS       XP_077504984             103 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114858 [Amblyomma americanum].
ACCESSION   XP_077504984
VERSION     XP_077504984.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648858.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..103
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..103
                     /product="uncharacterized protein LOC144114858"
                     /calculated_mol_wt=11166
     CDS             1..103
                     /gene="LOC144114858"
                     /coded_by="XM_077648858.1:80..391"
                     /db_xref="GeneID:144114858"
ORIGIN      
        1 mkvlfvcglv lagaffvkag qelcgldhdq ikatlkcmge hvnselkgka seiihsqsdn
       61 laelvkrqce aevdfgellk tvfseeeaea ikeaykkckp ghs
//
LOCUS       XP_077504986             476 aa            linear   INV 25-NOV-2025
DEFINITION  alanine aminotransferase 2-like [Amblyomma americanum].
ACCESSION   XP_077504986
VERSION     XP_077504986.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648860.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..476
                     /product="alanine aminotransferase 2-like"
                     /calculated_mol_wt=53273
     Region          33..476
                     /region_name="PTZ00377"
                     /note="alanine aminotransferase; Provisional"
                     /db_xref="CDD:240391"
     CDS             1..476
                     /gene="LOC144114859"
                     /coded_by="XM_077648860.1:13..1443"
                     /db_xref="GeneID:144114859"
ORIGIN      
        1 mstdelettr nvgqritgsn alkgiselmr wteaelkegk klgfadlira disdgqafgq
       61 kpltfprqvv cacafpdiad hmplphdvvs raneilhncp gntvgsvilg ghptvrrhva
      121 dyishrdsve vnpenvwitn gvfhgvslvl dalkrrsngk ppgvlaclpl ytsyfsmlqd
      181 kgyyeayyyl yedrnwshnv swmqealdes rkhctprflf lvnpsnppgt vlskneiegv
      241 irfahknklt iladevfehn vynnrrpfht vrkilnamga pyramplisf ssiskgfaae
      301 gglqsgyfea inldpvdean lehimeevlp pmlsqvaldc fvrppdpgep shdvyfkeka
      361 vilsslaera klaehrlnti egiqcspvha tmaayprisi pqkavehals qgkepdvfya
      421 eellrqkgvc vtpgsafgrl pgryyirvti lppkdklvel fdrlesfhee flakys
//
LOCUS       XP_077504987             123 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114860 [Amblyomma americanum].
ACCESSION   XP_077504987
VERSION     XP_077504987.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648861.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..123
                     /product="uncharacterized protein LOC144114860"
                     /calculated_mol_wt=13363
     CDS             1..123
                     /gene="LOC144114860"
                     /coded_by="XM_077648861.1:70..441"
                     /db_xref="GeneID:144114860"
ORIGIN      
        1 mnsrtlvglm lvgllgpasa ddhehhgsvc rapefhgsvc ameaeaqval vgciqehlde
       61 atatklntvk erlecdqlhc vfqkmcernq gtlehsgnef ftdaekeafr naisacretv
      121 lha
//
LOCUS       XP_077504988             113 aa            linear   INV 25-NOV-2025
DEFINITION  antimicrobial peptide microplusin-like [Amblyomma americanum].
ACCESSION   XP_077504988
VERSION     XP_077504988.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648862.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..113
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..113
                     /product="antimicrobial peptide microplusin-like"
                     /calculated_mol_wt=12289
     CDS             1..113
                     /gene="LOC144114861"
                     /coded_by="XM_077648862.1:73..414"
                     /db_xref="GeneID:144114861"
ORIGIN      
        1 mnsrtlvgim lvgllglasa dhhehhgsvc ameadaqval vgciqehlde atatklntvk
       61 erlecdqllc vfqkmcernq gtlehsgnef ftdaekegfr naisacretv hha
//
LOCUS       XP_077504989             113 aa            linear   INV 25-NOV-2025
DEFINITION  antimicrobial peptide microplusin-like [Amblyomma americanum].
ACCESSION   XP_077504989
VERSION     XP_077504989.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648863.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..113
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..113
                     /product="antimicrobial peptide microplusin-like"
                     /calculated_mol_wt=12382
     CDS             1..113
                     /gene="LOC144114862"
                     /coded_by="XM_077648863.1:73..414"
                     /db_xref="GeneID:144114862"
ORIGIN      
        1 mnsrtlvglm lvgllglasa dhhehhgsvc ameadaqval vgciqehlde atatklntvk
       61 erleceqlhc vfqkmcernq gtlehsgnef ftdaekegfr naisacrerv hha
//
LOCUS       XP_077504990             113 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114863 [Amblyomma americanum].
ACCESSION   XP_077504990
VERSION     XP_077504990.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648864.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..113
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..113
                     /product="uncharacterized protein LOC144114863"
                     /calculated_mol_wt=12317
     CDS             1..113
                     /gene="LOC144114863"
                     /coded_by="XM_077648864.1:70..411"
                     /db_xref="GeneID:144114863"
ORIGIN      
        1 mnprtlvglm lvgllglasa dhhehhgsvc alegeaqval vgciqehlde aarnklntvk
       61 erlecdqlhc vflkicernn gtlehsgnef ftdaekeglr naviacrdra hha
//
LOCUS       XP_077504991             130 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114864 [Amblyomma americanum].
ACCESSION   XP_077504991
VERSION     XP_077504991.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648865.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..130
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..130
                     /product="uncharacterized protein LOC144114864"
                     /calculated_mol_wt=14863
     CDS             1..130
                     /gene="LOC144114864"
                     /coded_by="XM_077648865.1:1240..1632"
                     /db_xref="GeneID:144114864"
ORIGIN      
        1 mkatviivvf mttavwfvss dpldlcgrpv pelsflmnci rlkttpsfrs llskfltkth
       61 ctndvcfvla wcrsnnhdfk gkafkampyk yflelfnaak kcearrppqe rpvgdeiwrg
      121 fmkilgaiff
//
LOCUS       XP_077504992             170 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114865 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077504992
VERSION     XP_077504992.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648866.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..170
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..170
                     /product="uncharacterized protein LOC144114865 isoform X1"
                     /calculated_mol_wt=19252
     CDS             1..170
                     /gene="LOC144114865"
                     /coded_by="XM_077648866.1:2195..2707"
                     /db_xref="GeneID:144114865"
ORIGIN      
        1 msssrsaapk iprwnpvslh srscirfgsg ackvpnnhve srqkeiinlf kcimarasae
       61 lqdqlkelkp klyctdyyiv vkkvcrlkpf aafmreylst palysemenl aeqcqkrgap
      121 ahsrpvqkqe teapglwqri tkylkklavm aadmmrgivr rftsnntasi
//
LOCUS       XP_077504993             159 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114865 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077504993
VERSION     XP_077504993.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648867.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..159
                     /product="uncharacterized protein LOC144114865 isoform X2"
                     /calculated_mol_wt=17825
     CDS             1..159
                     /gene="LOC144114865"
                     /coded_by="XM_077648867.1:1870..2349"
                     /db_xref="GeneID:144114865"
ORIGIN      
        1 mnvrltavvf iiaagaaaag pldlcdhves rqkeiinlfk cimarasael qdqlkelkpk
       61 lyctdyyivv kkvcrlkpfa afmreylstp alysemenla eqcqkrgapa hsrpvqkqet
      121 eapglwqrit kylkklavma admmrgivrr ftsnntasi
//
LOCUS       XP_077504994             136 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114865 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077504994
VERSION     XP_077504994.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648868.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..136
                     /product="uncharacterized protein LOC144114865 isoform X3"
                     /calculated_mol_wt=15245
     CDS             1..136
                     /gene="LOC144114865"
                     /coded_by="XM_077648868.1:79..489"
                     /db_xref="GeneID:144114865"
ORIGIN      
        1 mkfvlfiwll lqtavaprpp ldlctpdlph qrfhvgilfl cirgaasdll vqqlnhlknd
       61 vlhcrddfcl vetlcqrgql eaelarylta dnlmreircl akfcttpsdn kqaqqggtdy
      121 tdgifdvprc snsivs
//
LOCUS       XP_077504995             107 aa            linear   INV 25-NOV-2025
DEFINITION  antimicrobial peptide microplusin-like [Amblyomma americanum].
ACCESSION   XP_077504995
VERSION     XP_077504995.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648869.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..107
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..107
                     /product="antimicrobial peptide microplusin-like"
                     /calculated_mol_wt=11393
     CDS             1..107
                     /gene="LOC144114868"
                     /coded_by="XM_077648869.1:85..408"
                     /db_xref="GeneID:144114868"
ORIGIN      
        1 mnaflvcall vaftavsfah hlelcdkteg qlkselqcir rsisavanqs fnnaattlgc
       61 qdsacvirkl cvgndlekam akhfarsqit eihnaatacd psaghhh
//
LOCUS       XP_077504996             107 aa            linear   INV 25-NOV-2025
DEFINITION  antimicrobial peptide microplusin-like [Amblyomma americanum].
ACCESSION   XP_077504996
VERSION     XP_077504996.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648870.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..107
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..107
                     /product="antimicrobial peptide microplusin-like"
                     /calculated_mol_wt=11453
     CDS             1..107
                     /gene="LOC144114869"
                     /coded_by="XM_077648870.1:83..406"
                     /db_xref="GeneID:144114869"
ORIGIN      
        1 mkaflacvll aafaalsyah hlelcdkndg qlkselqcir rsiseaanrs fdnaarslac
       61 qdsacvirkl cvgndlekdm aehftgsqia enhkaatacd psaghhh
//
LOCUS       XP_077504998             107 aa            linear   INV 25-NOV-2025
DEFINITION  antimicrobial peptide microplusin-like [Amblyomma americanum].
ACCESSION   XP_077504998
VERSION     XP_077504998.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648872.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..107
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..107
                     /product="antimicrobial peptide microplusin-like"
                     /calculated_mol_wt=11698
     CDS             1..107
                     /gene="LOC144114870"
                     /coded_by="XM_077648872.1:82..405"
                     /db_xref="GeneID:144114870"
ORIGIN      
        1 mkaflvcvll atfaalsfah hlelcgkndg qlkrelqcir rsisaaanqs fdnavrdlrc
       61 rdnscvirkl cvgndlekam akyftraqit eihnaatacd psgghhh
//
LOCUS       XP_077504999             107 aa            linear   INV 25-NOV-2025
DEFINITION  antimicrobial peptide microplusin-like [Amblyomma americanum].
ACCESSION   XP_077504999
VERSION     XP_077504999.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648873.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..107
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..107
                     /product="antimicrobial peptide microplusin-like"
                     /calculated_mol_wt=11802
     CDS             1..107
                     /gene="LOC144114871"
                     /coded_by="XM_077648873.1:79..402"
                     /db_xref="GeneID:144114871"
ORIGIN      
        1 mkaflvcmll atfaalsfah hlelcdkndg qlkrelqcir rsisaaanqs fdnaardlrc
       61 rdnscvirkl cvgndlekam akyftrvqit eihnaatacd psaghhh
//
LOCUS       XP_077505000             110 aa            linear   INV 25-NOV-2025
DEFINITION  antimicrobial peptide microplusin-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505000
VERSION     XP_077505000.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648874.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..110
                     /product="antimicrobial peptide microplusin-like isoform
                     X1"
                     /calculated_mol_wt=12164
     CDS             1..110
                     /gene="LOC144114872"
                     /coded_by="XM_077648874.1:344..676"
                     /db_xref="GeneID:144114872"
ORIGIN      
        1 mkaflvcvll atfvalsfah hlelcdkndv qlkselqcir rsisaaaiqs fdnaardlrc
       61 rdnscvirrl cvgndlekam akyftraqit efrnasaacg fnltsderss
//
LOCUS       XP_077505001             107 aa            linear   INV 25-NOV-2025
DEFINITION  antimicrobial peptide microplusin-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505001
VERSION     XP_077505001.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648875.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..107
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..107
                     /product="antimicrobial peptide microplusin-like isoform
                     X2"
                     /calculated_mol_wt=11798
     CDS             1..107
                     /gene="LOC144114872"
                     /coded_by="XM_077648875.1:76..399"
                     /db_xref="GeneID:144114872"
ORIGIN      
        1 mkaflvcvll atfvalsfah hlelcdkndv qlkselqcir rsisaaaiqs fdnaardlrc
       61 rdnscvirrl cvgndlekam akyftrvqit eihnaatacd psaghhh
//
LOCUS       XP_077505002             110 aa            linear   INV 25-NOV-2025
DEFINITION  antimicrobial peptide microplusin-like [Amblyomma americanum].
ACCESSION   XP_077505002
VERSION     XP_077505002.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648876.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..110
                     /product="antimicrobial peptide microplusin-like"
                     /calculated_mol_wt=12204
     CDS             1..110
                     /gene="LOC144114873"
                     /coded_by="XM_077648876.1:182..514"
                     /db_xref="GeneID:144114873"
ORIGIN      
        1 mkaflvcvll atfaalsfag hlelcdktde qlkfevlcir liistaanvr ffaagkdldc
       61 rdttciirrl ctqndleram ekyftraqit elrnasaacg fnltsderss
//
LOCUS       XP_077505003             110 aa            linear   INV 25-NOV-2025
DEFINITION  antimicrobial peptide microplusin-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505003
VERSION     XP_077505003.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648877.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..110
                     /product="antimicrobial peptide microplusin-like isoform
                     X1"
                     /calculated_mol_wt=12220
     CDS             1..110
                     /gene="LOC144114874"
                     /coded_by="XM_077648877.1:52..384"
                     /db_xref="GeneID:144114874"
ORIGIN      
        1 mkaflvfall dtfaalscad hlelcdktde qlkfevlcir liistaanvr ffaagkdldc
       61 rdttciirrl ctqndleram akyftraqit elrnasaacg fnltsderss
//
LOCUS       XP_077505004              80 aa            linear   INV 25-NOV-2025
DEFINITION  antimicrobial peptide microplusin-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505004
VERSION     XP_077505004.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648878.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..80
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..80
                     /product="antimicrobial peptide microplusin-like isoform
                     X2"
                     /calculated_mol_wt=8808
     CDS             1..80
                     /gene="LOC144114874"
                     /coded_by="XM_077648878.1:52..294"
                     /db_xref="GeneID:144114874"
ORIGIN      
        1 mkaflvfall dtfaalscad hlelcdktde qlkfevlcir liistaeram akyftraqit
       61 elrnasaacg fnltsderss
//
LOCUS       XP_077505005             107 aa            linear   INV 25-NOV-2025
DEFINITION  antimicrobial peptide microplusin-like [Amblyomma americanum].
ACCESSION   XP_077505005
VERSION     XP_077505005.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648879.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..107
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..107
                     /product="antimicrobial peptide microplusin-like"
                     /calculated_mol_wt=11728
     CDS             1..107
                     /gene="LOC144114875"
                     /coded_by="XM_077648879.1:81..404"
                     /db_xref="GeneID:144114875"
ORIGIN      
        1 mkaflvcvll atfvalsfah hlelcdkndg qlkselqcir rsisaaaiqs fdnaardlrc
       61 rdnscvirrl cvgndlekam akyftraqit eihnaatacd psaghhh
//
LOCUS       XP_077505006             107 aa            linear   INV 25-NOV-2025
DEFINITION  antimicrobial peptide microplusin-like [Amblyomma americanum].
ACCESSION   XP_077505006
VERSION     XP_077505006.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648880.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..107
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..107
                     /product="antimicrobial peptide microplusin-like"
                     /calculated_mol_wt=11728
     CDS             1..107
                     /gene="LOC144114876"
                     /coded_by="XM_077648880.1:62..385"
                     /db_xref="GeneID:144114876"
ORIGIN      
        1 mkaflvcvll atfvalsfah hlelcdkndg qlkselqcir rsisaaaiqs fdnaardlrc
       61 rdnscvirrl cvgndlekam akyftraqit eihnaatacd psaghhh
//
LOCUS       XP_077505007             107 aa            linear   INV 25-NOV-2025
DEFINITION  antimicrobial peptide microplusin-like [Amblyomma americanum].
ACCESSION   XP_077505007
VERSION     XP_077505007.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648881.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..107
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..107
                     /product="antimicrobial peptide microplusin-like"
                     /calculated_mol_wt=11656
     CDS             1..107
                     /gene="LOC144114877"
                     /coded_by="XM_077648881.1:109..432"
                     /db_xref="GeneID:144114877"
ORIGIN      
        1 mkaflvcvll atfvalsfah hlelcdkndg qlkselqcir rsisaaaiqs fdnaardlrc
       61 rdnscvirgl cvgndlekam akyftrvqit eihnaatacd psaghhh
//
LOCUS       XP_077505008             121 aa            linear   INV 25-NOV-2025
DEFINITION  antimicrobial peptide microplusin-like [Amblyomma americanum].
ACCESSION   XP_077505008
VERSION     XP_077505008.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648882.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..121
                     /product="antimicrobial peptide microplusin-like"
                     /calculated_mol_wt=13070
     CDS             1..121
                     /gene="LOC144114878"
                     /coded_by="XM_077648882.1:81..446"
                     /db_xref="GeneID:144114878"
ORIGIN      
        1 mkavlasslf vaalvaatsa hhlelckknd qvlateleci kqhipestna afddtvqkle
       61 csdrscairk mcegndleaa makfftaeqi khvhdaattc dpdahhdhgh dhghdhgdgh
      121 h
//
LOCUS       XP_077505009             695 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114879 [Amblyomma americanum].
ACCESSION   XP_077505009
VERSION     XP_077505009.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648883.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..695
                     /product="uncharacterized protein LOC144114879"
                     /calculated_mol_wt=77854
     Region          237..380
                     /region_name="Transposase_21"
                     /note="Transposase family tnp2; cl29371"
                     /db_xref="CDD:475188"
     CDS             1..695
                     /gene="LOC144114879"
                     /coded_by="XM_077648883.1:36..2123"
                     /db_xref="GeneID:144114879"
ORIGIN      
        1 msmetevmsk wrryriieke yrellgelgi rrppsthlks sgskrtggea aldvvsappl
       61 plpsgaskrp nseayasgac asgsgratss lpadpsmpsv qselneqayv sescaeslss
      121 ehtatscdsr detsstnslt aenvemgqpr eespelcyrh lstaqelasi askhnlthac
      181 indlldfcrr rgivelpkda rtimqterka nlehgdsfvh fglaegiqha vgqgpapenv
      241 elhanidglp lhrssqtalw pilcrvinik ksepfmvsay cgagkppclq eylrpfidev
      301 rnlscnglmv kgvhvnvclk avicdapars fvkaitghtg yhacercsqk ghylenrmtf
      361 pdlhapqrtd ssfrsqedrr hhtgvspfts lnvdmvkcfp teymhlvclg vmrrllrnwi
      421 ckghaarlsr adrsalndkl reaatafpky fqrkprgtee ldrwkatefr tfllylgpvl
      481 lksilpdely khflylhvsv rvlaspqhhr dlnqfahdll ryfvqefgrl ygqkqlvynv
      541 htlahlaeqc lehgtvdefs afpfesylgk vkkrlrttnk plaqlsrrms evractplst
      601 gqvggdvkvg dcflvdncav vivdllgcna kagilpngre ffrvpidssr mdvrrysalg
      661 qetkiwpisy iknkvrcikl qykrghvvfp llhlq
//
LOCUS       XP_077505010             442 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114880 [Amblyomma americanum].
ACCESSION   XP_077505010
VERSION     XP_077505010.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648884.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..442
                     /product="uncharacterized protein LOC144114880"
                     /calculated_mol_wt=46904
     Region          359..>442
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     CDS             1..442
                     /gene="LOC144114880"
                     /coded_by="XM_077648884.1:1958..3286"
                     /db_xref="GeneID:144114880"
ORIGIN      
        1 mppkpfavvk fpmeddslav vpvgwlshdw qhchwpkrra aevirlaree adpsapgcew
       61 frcpvavvtt crsyknaekk akkyemssav essaisgneg pdgpdvrlps ppprlpsppp
      121 rlpsppprlp spppqlpspp rlisppslls shsmeaatpa aanctagcpa gsastagdle
      181 dveeatsdpt mlkvlrllle irqqqreilq rvsrleqarq eprtrspspp vsslpplcpq
      241 lpafsiedfe aaeaavrddr vaaalkkqll rlggnnlkev aanvmgavmg vavqrlfslr
      301 grkgkrpfvg tklcrvatda icerqgvdil aaqgfigrwl pgacdrgggr krryaqtlep
      361 peshaqappa npcagsapcp gapsascpat ppgmailsps gvhpssappp hsllltpshp
      421 ppklcpfpcp phsllvtpsh pt
//
LOCUS       XP_077505011             804 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114881 [Amblyomma americanum].
ACCESSION   XP_077505011
VERSION     XP_077505011.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648885.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..804
                     /product="uncharacterized protein LOC144114881"
                     /calculated_mol_wt=80029
     Region          29..>71
                     /region_name="Collagen"
                     /note="Collagen triple helix repeat (20 copies);
                     pfam01391"
                     /db_xref="CDD:460189"
     Region          <116..>357
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <286..>492
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          579..803
                     /region_name="COLFI"
                     /note="Fibrillar collagen C-terminal domain; cl02436"
                     /db_xref="CDD:470578"
     CDS             1..804
                     /gene="LOC144114881"
                     /coded_by="XM_077648885.1:177..2591"
                     /db_xref="GeneID:144114881"
ORIGIN      
        1 mgppgfpgpk geiglpgalg prgdkgdigl pgdpgpagek grsgpvgpag pigpkgdkgd
       61 iggpgplgrd glpgarglpg ppgpvgppge dgdkgdsglp gqkglkggkg dtgllgppgp
      121 qglrgepgvp gadgepgppg pagprgtkge dgpigavgsa gapgpqglpg prgvkgdkgd
      181 vgkkgppglp gmtgeqgpvg akggegipgs pgpegpqgqk gddgppglpg ptgkdgepgp
      241 rgppgpkgee gkpglqgvpg vkgppglegp kgspgppgfp gpsgeaglpg ikgergadgv
      301 dgqpgepgpm gakgemgkmg ipgaagkpgp mgpaglqgdv glpgekgdkg qvgpeglqgp
      361 tgppgppgpt gptglrgspg pagdvgppgl vgapgqpgnv gapgdkgmkg drgkrglkgh
      421 sgllgrtgak gdsgekgekg qrgdtgppgp pgaqgpvgme gpkgsdgpqg epgpegipgp
      481 kgvqgpagmk getgpqgpag ppgppgegpq ippemlfqrd lhnrfkrsle kvdnasteed
      541 yavitgddgn ddddknstaa aepvdevaye kinrtlthif anvyilrrei eqirrpvgsr
      601 pnpsrscrdl rlahpdspdg wywvdpnlgv pddavrvfcg mraggetcvf adgdtdsarl
      661 srwekpasas aasntsswfs slkggfrlry rtvgdvqlrf lsflstsayq nftfvcnnvn
      721 awysqrtkdh aralrfrgrn eaqmsfekds ptvpvddcqf rdgktvfvfk tkqtatlpiv
      781 dfqpfdfgge hqsfgfeigp vcfq
//
LOCUS       XP_077505012             607 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114882 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505012
VERSION     XP_077505012.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648886.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..607
                     /product="uncharacterized protein LOC144114882 isoform X1"
                     /calculated_mol_wt=62528
     Region          93..254
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cl22861"
                     /db_xref="CDD:473984"
     Region          <349..>593
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     CDS             1..607
                     /gene="LOC144114882"
                     /coded_by="XM_077648886.1:130..1953"
                     /db_xref="GeneID:144114882"
ORIGIN      
        1 mageacshgs kpaagpmrrr rrrrrssgar eaglaclllc lhaagmtsaq lvpdtvdlvg
       61 rvdlgllppg vevrpgiceq rpawldadgt pmflgpdtay glsraanlsv ptaeafsrgf
      121 prdfsvlatf rieqntrgyl lavydwddre qlslhvgenl tfalrstngn gsavseavfa
      181 eafndgkwhr malsvkgdav tlvkdcvvre teemarsdda lvdtsgvtwv gqaldadvdf
      241 fqgdlqqllv vpapdsayel cssyvpgcai plppldsdyg dplfnrtdpv ekephprptg
      301 gfynettpyp hypdgevtpv ppyhpvityp vegppgpqgf pgspglpgfk gdkgdpgrdg
      361 lagtvgqpga pghifmipyq tagstkgpdn teslrqmlsq hmlamrgapg pmgltglhgp
      421 vgpmgqpgek gergeggavg prgmrgqqgt agkpgrrgrg gkdgergkpg ppgpkgdvgl
      481 pglpglpgek gergyvgspg dqglvghegh pgddgpqglp glpgemgprg fpgprgfagl
      541 pgppgipgse gpvgpkgspg ppgiagqpgr qgmqgpvgsp gpqgvpgplg ppvgneasfe
      601 ntqalra
//
LOCUS       XP_077505013             565 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114882 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505013
VERSION     XP_077505013.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648887.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..565
                     /product="uncharacterized protein LOC144114882 isoform X2"
                     /calculated_mol_wt=58614
     Region          93..254
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cl22861"
                     /db_xref="CDD:473984"
     Region          <349..>536
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     CDS             1..565
                     /gene="LOC144114882"
                     /coded_by="XM_077648887.1:130..1827"
                     /db_xref="GeneID:144114882"
ORIGIN      
        1 mageacshgs kpaagpmrrr rrrrrssgar eaglaclllc lhaagmtsaq lvpdtvdlvg
       61 rvdlgllppg vevrpgiceq rpawldadgt pmflgpdtay glsraanlsv ptaeafsrgf
      121 prdfsvlatf rieqntrgyl lavydwddre qlslhvgenl tfalrstngn gsavseavfa
      181 eafndgkwhr malsvkgdav tlvkdcvvre teemarsdda lvdtsgvtwv gqaldadvdf
      241 fqgdlqqllv vpapdsayel cssyvpgcai plppldsdyg dplfnrtdpv ekephprptg
      301 gfynettpyp hypdgevtpv ppyhpvityp vegppgpqgf pgspglpgfk gdkgdpgrdg
      361 lagtvgqpga pghifmipyq tagstkgpdn teslrqmlsq hmlamrgapg pmgltglhgp
      421 vgpmgqpgek gergeggavg prgmrgqqgt agkpgrrgrg gkdgergkpg ppgpkgdvgl
      481 pglpglpgek gergyvgspg dqglvghegh pgddgpqglp glpgemgprg fpgprgfagl
      541 pgppgipgse gpvgpkgspv regti
//
LOCUS       XP_077505014             820 aa            linear   INV 25-NOV-2025
DEFINITION  dual specificity protein kinase monopolar spindle 1 isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077505014
VERSION     XP_077505014.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648888.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..820
                     /product="dual specificity protein kinase monopolar
                     spindle 1 isoform X1"
                     /calculated_mol_wt=90246
     Region          509..778
                     /region_name="PKc_Mps1"
                     /note="Catalytic domain of the Dual-specificity Mitotic
                     checkpoint protein kinase, Monopolar spindle 1 (also
                     called TTK); cd14131"
                     /db_xref="CDD:271033"
     Site            order(516..520,524,536,538,570,588..591,594,596,636,638,
                     640..641,643,653,656,674..677)
                     /site_type="active"
                     /db_xref="CDD:271033"
     Site            order(516..520,524,536,538,570,588..591,594,636,638,
                     640..641,643,653)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271033"
     Site            order(520,594,596,636,638,640,656,674..677)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271033"
     Site            order(522,538,540,553,556,586,652)
                     /site_type="other"
                     /note="secondary chemical binding site [chemical binding]"
                     /db_xref="CDD:271033"
     Site            652..677
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271033"
     CDS             1..820
                     /gene="Mps1"
                     /coded_by="XM_077648888.1:168..2630"
                     /db_xref="GeneID:144114883"
ORIGIN      
        1 mhaatpaseg raspamlpss gpqyscqake sshgyaksrp tpsslrtlkh rtptkrkepv
       61 pgrhaglqhg qlrtpaaqsp leletpfemr naknspekst fsmsrkgtpq fsgskmsspa
      121 dfleerrrsv sktpeppspv rppsshsslq wlrgcldkdk nstsqsglkl syaaaaaggs
      181 aqlltpahsq gsssgtlrtq ttqylrcqer rpagniyqrw khavlvlwdl wktaaewwtr
      241 fwssfpllrl halsngrnlv eeafdaacpg tgfssrsstc wskdssqsss tkktgtppal
      301 rkfkedpgff kqrlplsesd esdddagdqp vpaepprgsr lercpsrntp lkksppknsl
      361 lrsstfersa skmppssqrt vahskqqswq qsskslsaar tcaqakppqa ahaqqsgrqr
      421 hssycpprts aretrepaia ensaehvpsv aadrkvgkaa avgqngslrs rlsganevlp
      481 gsqrrsllsl lqdsdppvgn yhteingnry qvlnligrgg sskvfmmlty rkklcavklv
      541 smsgvqpvvv qaymnevail kslrdcervv rlydyeyvrk dqvlalvmek gdqdlssiit
      601 aaikkgaldp tgvkfywfem lqavreihek gvihsdlkpa nflfvdgklk lidfgiastt
      661 qadvtsvfke spmgtlnfms peslqlvdhe agkhclkisr ksdvwslgci lhhlvlgytp
      721 fqhiqstaak mtaiagasyv infpeisdpn lldvlkkclq rnpnqrptiq elldhpyltk
      781 dgakqrnpvl qsvlteienl spasvvklsk vvkylrqqkk
//
LOCUS       XP_077505015             813 aa            linear   INV 25-NOV-2025
DEFINITION  dual specificity protein kinase monopolar spindle 1 isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077505015
VERSION     XP_077505015.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648889.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..813
                     /product="dual specificity protein kinase monopolar
                     spindle 1 isoform X2"
                     /calculated_mol_wt=89417
     Region          502..771
                     /region_name="PKc_Mps1"
                     /note="Catalytic domain of the Dual-specificity Mitotic
                     checkpoint protein kinase, Monopolar spindle 1 (also
                     called TTK); cd14131"
                     /db_xref="CDD:271033"
     Site            order(509..513,517,529,531,563,581..584,587,589,629,631,
                     633..634,636,646,649,667..670)
                     /site_type="active"
                     /db_xref="CDD:271033"
     Site            order(509..513,517,529,531,563,581..584,587,629,631,
                     633..634,636,646)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271033"
     Site            order(513,587,589,629,631,633,649,667..670)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271033"
     Site            order(515,531,533,546,549,579,645)
                     /site_type="other"
                     /note="secondary chemical binding site [chemical binding]"
                     /db_xref="CDD:271033"
     Site            645..670
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271033"
     CDS             1..813
                     /gene="Mps1"
                     /coded_by="XM_077648889.1:168..2609"
                     /db_xref="GeneID:144114883"
ORIGIN      
        1 mhaatpaseg raspamlpss gpqyscqake sshgyaksrp tpsslrtlkh rtptkrkepv
       61 pgrhaglqhg qlrtpaaqsp leletpfemr naknspekst fsmsrkgtpq fsgskmsspa
      121 dfleerrrsv sktpeppspv rppsshsslq wlrgcldkdk nstsqsglkl syaaaaaggs
      181 aqlltpahsq gsssgtylrc qerrpagniy qrwkhavlvl wdlwktaaew wtrfwssfpl
      241 lrlhalsngr nlveeafdaa cpgtgfssrs stcwskdssq ssstkktgtp palrkfkedp
      301 gffkqrlpls esdesdddag dqpvpaeppr gsrlercpsr ntplkksppk nsllrsstfe
      361 rsaskmppss qrtvahskqq swqqssksls aartcaqakp pqaahaqqsg rqrhssycpp
      421 rtsaretrep aiaensaehv psvaadrkvg kaaavgqngs lrsrlsgane vlpgsqrrsl
      481 lsllqdsdpp vgnyhteing nryqvlnlig rggsskvfmm ltyrkklcav klvsmsgvqp
      541 vvvqaymnev ailkslrdce rvvrlydyey vrkdqvlalv mekgdqdlss iitaaikkga
      601 ldptgvkfyw femlqavrei hekgvihsdl kpanflfvdg klklidfgia sttqadvtsv
      661 fkespmgtln fmspeslqlv dheagkhclk isrksdvwsl gcilhhlvlg ytpfqhiqst
      721 aakmtaiaga syvinfpeis dpnlldvlkk clqrnpnqrp tiqelldhpy ltkdgakqrn
      781 pvlqsvltei enlspasvvk lskvvkylrq qkk
//
LOCUS       XP_077505016             808 aa            linear   INV 25-NOV-2025
DEFINITION  dual specificity protein kinase monopolar spindle 1 isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077505016
VERSION     XP_077505016.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648890.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..808
                     /product="dual specificity protein kinase monopolar
                     spindle 1 isoform X3"
                     /calculated_mol_wt=88753
     Region          497..766
                     /region_name="PKc_Mps1"
                     /note="Catalytic domain of the Dual-specificity Mitotic
                     checkpoint protein kinase, Monopolar spindle 1 (also
                     called TTK); cd14131"
                     /db_xref="CDD:271033"
     Site            order(504..508,512,524,526,558,576..579,582,584,624,626,
                     628..629,631,641,644,662..665)
                     /site_type="active"
                     /db_xref="CDD:271033"
     Site            order(504..508,512,524,526,558,576..579,582,624,626,
                     628..629,631,641)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271033"
     Site            order(508,582,584,624,626,628,644,662..665)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271033"
     Site            order(510,526,528,541,544,574,640)
                     /site_type="other"
                     /note="secondary chemical binding site [chemical binding]"
                     /db_xref="CDD:271033"
     Site            640..665
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271033"
     CDS             1..808
                     /gene="Mps1"
                     /coded_by="XM_077648890.1:168..2594"
                     /db_xref="GeneID:144114883"
ORIGIN      
        1 mhaatpaseg raspamlpss gpqyscqake sshgyaksrp tpsslrtlkh rtptkrkepv
       61 pgrhaglqhg qlrtpaaqsp leletpfemr naknspekst fsmsrkgtpq fsgskmsspa
      121 dfleerrrsv sktpeppspv rppsshsslq wlrgcldkdk nstsqsglkl syaaaaaggs
      181 aqlltpahsq gsssgterrp agniyqrwkh avlvlwdlwk taaewwtrfw ssfpllrlha
      241 lsngrnlvee afdaacpgtg fssrsstcws kdssqssstk ktgtppalrk fkedpgffkq
      301 rlplsesdes dddagdqpvp aepprgsrle rcpsrntplk ksppknsllr sstfersask
      361 mppssqrtva hskqqswqqs skslsaartc aqakppqaah aqqsgrqrhs sycpprtsar
      421 etrepaiaen saehvpsvaa drkvgkaaav gqngslrsrl sganevlpgs qrrsllsllq
      481 dsdppvgnyh teingnryqv lnligrggss kvfmmltyrk klcavklvsm sgvqpvvvqa
      541 ymnevailks lrdcervvrl ydyeyvrkdq vlalvmekgd qdlssiitaa ikkgaldptg
      601 vkfywfemlq avreihekgv ihsdlkpanf lfvdgklkli dfgiasttqa dvtsvfkesp
      661 mgtlnfmspe slqlvdheag khclkisrks dvwslgcilh hlvlgytpfq hiqstaakmt
      721 aiagasyvin fpeisdpnll dvlkkclqrn pnqrptiqel ldhpyltkdg akqrnpvlqs
      781 vlteienlsp asvvklskvv kylrqqkk
//
LOCUS       XP_077505017             748 aa            linear   INV 25-NOV-2025
DEFINITION  dual specificity protein kinase monopolar spindle 1 isoform X4
            [Amblyomma americanum].
ACCESSION   XP_077505017
VERSION     XP_077505017.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648891.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..748
                     /product="dual specificity protein kinase monopolar
                     spindle 1 isoform X4"
                     /calculated_mol_wt=81587
     Region          437..706
                     /region_name="PKc_Mps1"
                     /note="Catalytic domain of the Dual-specificity Mitotic
                     checkpoint protein kinase, Monopolar spindle 1 (also
                     called TTK); cd14131"
                     /db_xref="CDD:271033"
     Site            order(444..448,452,464,466,498,516..519,522,524,564,566,
                     568..569,571,581,584,602..605)
                     /site_type="active"
                     /db_xref="CDD:271033"
     Site            order(444..448,452,464,466,498,516..519,522,564,566,
                     568..569,571,581)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271033"
     Site            order(448,522,524,564,566,568,584,602..605)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271033"
     Site            order(450,466,468,481,484,514,580)
                     /site_type="other"
                     /note="secondary chemical binding site [chemical binding]"
                     /db_xref="CDD:271033"
     Site            580..605
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271033"
     CDS             1..748
                     /gene="Mps1"
                     /coded_by="XM_077648891.1:168..2414"
                     /db_xref="GeneID:144114883"
ORIGIN      
        1 mhaatpaseg raspamlpss gpqyscqake sshgyaksrp tpsslrtlkh rtptkrkepv
       61 pgrhaglqhg qlrtpaaqsp leletpfemr naknspekst fsmsrkgtpq fsgskmsspa
      121 dfleerrrsv sktpeppspv rppsshsslq wlrgcldkdk nstsqsglkl syaaaaaggs
      181 aqlltpahsq gsssgtpgtg fssrsstcws kdssqssstk ktgtppalrk fkedpgffkq
      241 rlplsesdes dddagdqpvp aepprgsrle rcpsrntplk ksppknsllr sstfersask
      301 mppssqrtva hskqqswqqs skslsaartc aqakppqaah aqqsgrqrhs sycpprtsar
      361 etrepaiaen saehvpsvaa drkvgkaaav gqngslrsrl sganevlpgs qrrsllsllq
      421 dsdppvgnyh teingnryqv lnligrggss kvfmmltyrk klcavklvsm sgvqpvvvqa
      481 ymnevailks lrdcervvrl ydyeyvrkdq vlalvmekgd qdlssiitaa ikkgaldptg
      541 vkfywfemlq avreihekgv ihsdlkpanf lfvdgklkli dfgiasttqa dvtsvfkesp
      601 mgtlnfmspe slqlvdheag khclkisrks dvwslgcilh hlvlgytpfq hiqstaakmt
      661 aiagasyvin fpeisdpnll dvlkkclqrn pnqrptiqel ldhpyltkdg akqrnpvlqs
      721 vlteienlsp asvvklskvv kylrqqkk
//
LOCUS       XP_077505018             139 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114885 [Amblyomma americanum].
ACCESSION   XP_077505018
VERSION     XP_077505018.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648892.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..139
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..139
                     /product="uncharacterized protein LOC144114885"
                     /calculated_mol_wt=15708
     CDS             1..139
                     /gene="LOC144114885"
                     /coded_by="XM_077648892.1:1017..1436"
                     /db_xref="GeneID:144114885"
ORIGIN      
        1 mlfllaaahl sirerslway prpdswyett lphlsgsglr knfrinrgtf rymvavcgsm
       61 srqdtnmlqp iplekrlais lyrlatsaee rtaanlfgth lmklfpvqvp lgpphrpltt
      121 gcrvpdvfsk ffwtcqgtl
//
LOCUS       XP_077505019             123 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114886 [Amblyomma americanum].
ACCESSION   XP_077505019
VERSION     XP_077505019.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648893.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..123
                     /product="uncharacterized protein LOC144114886"
                     /calculated_mol_wt=13150
     CDS             1..123
                     /gene="LOC144114886"
                     /coded_by="XM_077648893.1:679..1050"
                     /db_xref="GeneID:144114886"
ORIGIN      
        1 mmgelslkfr ggnhmsegth cgwicskmks ccqlsmrhqt aqkcschgrr aclneaaett
       61 shnlhpaplf avpvlqylyc kaqataaatq aervlaqaav pvlaqapkeg npkraatsdk
      121 dll
//
LOCUS       XP_077505020            1281 aa            linear   INV 25-NOV-2025
DEFINITION  cell division cycle and apoptosis regulator protein 1-like isoform
            X1 [Amblyomma americanum].
ACCESSION   XP_077505020
VERSION     XP_077505020.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648894.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1281
                     /product="cell division cycle and apoptosis regulator
                     protein 1-like isoform X1"
                     /calculated_mol_wt=143117
     Region          136..193
                     /region_name="S1-like"
                     /note="pfam14444"
                     /db_xref="CDD:464176"
     Region          455..549
                     /region_name="BURAN"
                     /note="BURAN domain; pfam19257"
                     /db_xref="CDD:466013"
     Region          574..690
                     /region_name="DBC1"
                     /note="pfam14443"
                     /db_xref="CDD:464175"
     Region          733..766
                     /region_name="SAP"
                     /note="Putative DNA-binding (bihelical) motif predicted to
                     be involved in chromosomal organisation; smart00513"
                     /db_xref="CDD:128789"
     Region          735..>859
                     /region_name="PLN03124"
                     /note="poly [ADP-ribose] polymerase; Provisional"
                     /db_xref="CDD:215591"
     Region          873..940
                     /region_name="LAIKA"
                     /note="LAIKA domain; pfam19256"
                     /db_xref="CDD:466012"
     Region          1175..>1277
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1281
                     /gene="LOC144114887"
                     /coded_by="XM_077648894.1:107..3952"
                     /db_xref="GeneID:144114887"
ORIGIN      
        1 msqfggsgkn ppwmratelp lagsamlaqa gaslasalsq pqtavqqqqt qpfstnlaal
       61 glaqnalqat tvlsqpglai paslatstqa glglsaasla asggaglgyg ttqaaaarll
      121 gaaaattgvt spstkqrvft gtvtklhdnf gfvdedvffq tcvikgtmpk vgervlveas
      181 ynpnmpfkwn ayriqllanq mnasvgvrln nqitsssqss tsaamaaqtq yinsltsenk
      241 ssrsegekvs yaqlldrtes rhraeyansn arttarrssp srrdrdmrhd drrdryrdrn
      301 sgrdfdkdrr pydrnqdrlc drnsdrnqdr mqdrdraqdr driqdrdrdr mmdrdrgqdr
      361 lpdriqdrnr spirdhrsrd rdrdrerdrd lrglaqspdr dyrgsgqdhd gssssslark
      421 hplspprrgg ggspprrkpr mmpryvvdip avsldqrelg vvdlrkrypn lyipsdffha
      481 hicwqecfpl hrpfglnypc gfhvlpkdtc pplpsvvgsv qsdstllpav ldppdinfsf
      541 sakvmllssp sleelyqqtc alaedesrsg aalhparvls flvglkgkse tvaiggpwsp
      601 sldgpnpdsd pqtlirtavr tcraltgidl sactqwyrfa eicyrrenps pngakvptat
      661 lekvvlffpd vwrcmptrqe wadlelrlrn addtaaddsa tagdtaapsk eeesaaeerg
      721 ikepthyadl dpktmkvgel rveleargls tkglksqlva rlskalkaea emaegneedq
      781 eeeeveeaee dedgeepvee avldevkeev tdeikdedda drqeeekdad mtdkivdtvd
      841 vikketiegp kdealakatv pekvgtplrl edlpsepsil vypsrtakgg rfdcsvmsls
      901 llldyrqedn kehsfevslf aelfnemlir dfgfsiyral leapelkeek kkdtpkesek
      961 kgdrrddrks drrerrdrke dekktddqde sdaaseqsda egdseenkek rprkesraqq
     1021 qrtvqrhlll sfiyfdqnqc gylqdrdled ifyiiglels rgqvrrllqk vskretvryr
     1081 sltdapvcpe diveeapvad eemvddedgp vkeqpsqhdl aeialgnrgl llkpesgpqe
     1141 csvadpsaal apasasgakl vfhdgalldv gkilrqvers erartssetr lkeliealae
     1201 vresweasrq evlkmqield qtrrqlvtte ddlcssrkaa qelrlalqnc kqhlqatlgs
     1261 ldtiigrkeh radikkevmd d
//
LOCUS       XP_077505021            1267 aa            linear   INV 25-NOV-2025
DEFINITION  cell division cycle and apoptosis regulator protein 1-like isoform
            X2 [Amblyomma americanum].
ACCESSION   XP_077505021
VERSION     XP_077505021.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648895.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1267
                     /product="cell division cycle and apoptosis regulator
                     protein 1-like isoform X2"
                     /calculated_mol_wt=141808
     Region          122..179
                     /region_name="S1-like"
                     /note="pfam14444"
                     /db_xref="CDD:464176"
     Region          441..535
                     /region_name="BURAN"
                     /note="BURAN domain; pfam19257"
                     /db_xref="CDD:466013"
     Region          560..676
                     /region_name="DBC1"
                     /note="pfam14443"
                     /db_xref="CDD:464175"
     Region          719..752
                     /region_name="SAP"
                     /note="Putative DNA-binding (bihelical) motif predicted to
                     be involved in chromosomal organisation; smart00513"
                     /db_xref="CDD:128789"
     Region          721..>845
                     /region_name="PLN03124"
                     /note="poly [ADP-ribose] polymerase; Provisional"
                     /db_xref="CDD:215591"
     Region          859..926
                     /region_name="LAIKA"
                     /note="LAIKA domain; pfam19256"
                     /db_xref="CDD:466012"
     Region          1161..>1263
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1267
                     /gene="LOC144114887"
                     /coded_by="XM_077648895.1:107..3910"
                     /db_xref="GeneID:144114887"
ORIGIN      
        1 msqfggsgkn ppwmratelp lagsamlaqa gaslasalsq pqtavqqqqt qpfstnlaal
       61 glaqnalqat tvlsqaglgl saaslaasgg aglgygttqa aaarllgaaa attgvtspst
      121 kqrvftgtvt klhdnfgfvd edvffqtcvi kgtmpkvger vlveasynpn mpfkwnayri
      181 qllanqmnas vgvrlnnqit sssqsstsaa maaqtqyins ltsenkssrs egekvsyaql
      241 ldrtesrhra eyansnartt arrsspsrrd rdmrhddrrd ryrdrnsgrd fdkdrrpydr
      301 nqdrlcdrns drnqdrmqdr draqdrdriq drdrdrmmdr drgqdrlpdr iqdrnrspir
      361 dhrsrdrdrd rerdrdlrgl aqspdrdyrg sgqdhdgsss sslarkhpls pprrggggsp
      421 prrkprmmpr yvvdipavsl dqrelgvvdl rkrypnlyip sdffhahicw qecfplhrpf
      481 glnypcgfhv lpkdtcpplp svvgsvqsds tllpavldpp dinfsfsakv mllsspslee
      541 lyqqtcalae desrsgaalh parvlsflvg lkgksetvai ggpwspsldg pnpdsdpqtl
      601 irtavrtcra ltgidlsact qwyrfaeicy rrenpspnga kvptatlekv vlffpdvwrc
      661 mptrqewadl elrlrnaddt aaddsatagd taapskeees aaeergikep thyadldpkt
      721 mkvgelrvel earglstkgl ksqlvarlsk alkaeaemae gneedqeeee veeaeededg
      781 eepveeavld evkeevtdei kdeddadrqe eekdadmtdk ivdtvdvikk etiegpkdea
      841 lakatvpekv gtplrledlp sepsilvyps rtakggrfdc svmslsllld yrqednkehs
      901 fevslfaelf nemlirdfgf siyralleap elkeekkkdt pkesekkgdr rddrksdrre
      961 rrdrkedekk tddqdesdaa seqsdaegds eenkekrprk esraqqqrtv qrhlllsfiy
     1021 fdqnqcgylq drdledifyi iglelsrgqv rrllqkvskr etvryrsltd apvcpedive
     1081 eapvadeemv ddedgpvkeq psqhdlaeia lgnrglllkp esgpqecsva dpsaalapas
     1141 asgaklvfhd galldvgkil rqverserar tssetrlkel iealaevres weasrqevlk
     1201 mqieldqtrr qlvtteddlc ssrkaaqelr lalqnckqhl qatlgsldti igrkehradi
     1261 kkevmdd
//
LOCUS       XP_077505022             269 aa            linear   INV 25-NOV-2025
DEFINITION  aldo-keto reductase family 1 member B1-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505022
VERSION     XP_077505022.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648896.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..269
                     /product="aldo-keto reductase family 1 member B1-like
                     isoform X1"
                     /calculated_mol_wt=30556
     Region          <32..255
                     /region_name="AKR_SF"
                     /note="Aldo-keto reductase (AKR) superfamily; cl00470"
                     /db_xref="CDD:444925"
     Site            order(33,66,115..116,137,163..169,199,214..217,222,
                     225..226)
                     /site_type="active"
                     /db_xref="CDD:381296"
     CDS             1..269
                     /gene="LOC144114888"
                     /coded_by="XM_077648896.1:16..825"
                     /db_xref="GeneID:144114888"
ORIGIN      
        1 mptrptyvga siplprisls snhhhhltpr rsvlwctyys raraveccql slkllglnyl
       61 dlylmhwpfg ykegselfpr daageiinsd adyievwkgm edcvakglvr sigicnfnkq
      121 qmtrllesak vkpsvlqvec hpylnqgell sfcqkhnivl taysplgspd rpwakpdepv
      181 lledpaiksi aeahgktaaq vllryqverg vivipksvtk eriisnidif dfklspeemk
      241 tidgfnrnfr fvtvpwslkh kyypfhdaa
//
LOCUS       XP_077505023             318 aa            linear   INV 25-NOV-2025
DEFINITION  aldo-keto reductase family 1 member B1-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505023
VERSION     XP_077505023.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648897.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..318
                     /product="aldo-keto reductase family 1 member B1-like
                     isoform X2"
                     /calculated_mol_wt=35776
     Region          11..304
                     /region_name="AKR_SF"
                     /note="Aldo-keto reductase (AKR) superfamily; cl00470"
                     /db_xref="CDD:444925"
     Site            order(23..25,48,53,82,115,164..165,186,212..218,248,
                     263..266,271,274..275)
                     /site_type="active"
                     /db_xref="CDD:381296"
     Site            order(48,53,82,115)
                     /site_type="active"
                     /note="catalytic tetrad [active]"
                     /db_xref="CDD:381296"
     CDS             1..318
                     /gene="LOC144114888"
                     /coded_by="XM_077648897.1:221..1177"
                     /db_xref="GeneID:144114888"
ORIGIN      
        1 maldsvptit lstgykvpii glgtwksepg kveqavkiai dagyrhidca lvyenenevg
       61 raidakisda tvcredlwit sklwctyysr araveccqls lkllglnyld lylmhwpfgy
      121 kegselfprd aageiinsda dyievwkgme dcvakglvrs igicnfnkqq mtrllesakv
      181 kpsvlqvech pylnqgells fcqkhnivlt aysplgspdr pwakpdepvl ledpaiksia
      241 eahgktaaqv llryqvergv ivipksvtke riisnidifd fklspeemkt idgfnrnfrf
      301 vtvpwslkhk yypfhdaa
//
LOCUS       XP_077505024             203 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114889 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505024
VERSION     XP_077505024.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648898.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..203
                     /product="uncharacterized protein LOC144114889 isoform X1"
                     /calculated_mol_wt=21565
     CDS             1..203
                     /gene="LOC144114889"
                     /coded_by="XM_077648898.1:471..1082"
                     /db_xref="GeneID:144114889"
ORIGIN      
        1 mpaacssgps vegvpnapaa enipscsels sttvcarelp pslqscseeg ntseeapcgi
       61 ensstnslam qrviiehsrk tvllctsena altiekspsq mqgtishsll kfhkkpamkt
      121 dihtessvkq arddreetps tgacvqncis qplskkrqmd tpgsttmpst picsrtfdga
      181 gpqvesvwke metlkrklag cqk
//
LOCUS       XP_077505025             203 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114889 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505025
VERSION     XP_077505025.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648899.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..203
                     /product="uncharacterized protein LOC144114889 isoform X1"
                     /calculated_mol_wt=21565
     CDS             1..203
                     /gene="LOC144114889"
                     /coded_by="XM_077648899.1:325..936"
                     /db_xref="GeneID:144114889"
ORIGIN      
        1 mpaacssgps vegvpnapaa enipscsels sttvcarelp pslqscseeg ntseeapcgi
       61 ensstnslam qrviiehsrk tvllctsena altiekspsq mqgtishsll kfhkkpamkt
      121 dihtessvkq arddreetps tgacvqncis qplskkrqmd tpgsttmpst picsrtfdga
      181 gpqvesvwke metlkrklag cqk
//
LOCUS       XP_077505026             187 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114889 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505026
VERSION     XP_077505026.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648900.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="uncharacterized protein LOC144114889 isoform X2"
                     /calculated_mol_wt=19789
     CDS             1..187
                     /gene="LOC144114889"
                     /coded_by="XM_077648900.1:471..1034"
                     /db_xref="GeneID:144114889"
ORIGIN      
        1 mpaacssgps vegvpnapaa enipscsels sttvcarelp pslqscseeg ntseeapcgi
       61 ensstnslam qrviiehsrk tvllctsena altiekspsq mqetdihtes svkqarddre
      121 etpstgacvq ncisqplskk rqmdtpgstt mpstpicsrt fdgagpqves vwkemetlkr
      181 klagcqk
//
LOCUS       XP_077505027             704 aa            linear   INV 25-NOV-2025
DEFINITION  discoidin domain-containing receptor 2 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505027
VERSION     XP_077505027.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648901.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..704
                     /product="discoidin domain-containing receptor 2 isoform
                     X1"
                     /calculated_mol_wt=78852
     Region          398..695
                     /region_name="PTKc_DDR"
                     /note="Catalytic domain of the Protein Tyrosine Kinases,
                     Discoidin Domain Receptors; cd05051"
                     /db_xref="CDD:270644"
     Site            order(410..414,418,449,451,497..500,503..504,554,558..559,
                     561,572,590..594,603,638)
                     /site_type="active"
                     /db_xref="CDD:270644"
     Site            order(410..411,413..414,418,449,451,497..500,503..504,
                     558..559,561,572)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270644"
     Site            order(554,558,590..594,603,638)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270644"
     Site            571..596
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270644"
     CDS             1..704
                     /gene="Ddr"
                     /coded_by="XM_077648901.1:23..2137"
                     /db_xref="GeneID:144114891"
ORIGIN      
        1 malaegilay sipqgerrgt dvdlsdatyd gikddsylhg glgqltdgva gsenfkldvs
       61 gygkgyewvg wrndslhsqp lelvfefdtv rnfsavflhc nnmhtrdvqp fraarisfsv
      121 ggkhylgepq hfnlmpglfn ektrnvtirl hhhvgrfvkl qfefaakwll lsevsftsvp
      181 avgnfteeea paappivsta msedpmskqy vglvigvlaa villlvlvif vivarnrrrk
      241 hstphailkp ldsrvtinmk dlalayspqy vareangnly gqvaledpdp dkllyhepqd
      301 fkpiypgsys nesttsreya vpdmnkspvl sshksspfpq skpppppaae qyyaatdvvv
      361 slpniqgvsg ntvysvptld lssadensgg stlppvrevp rhrirfiekl gegqfgevga
      421 rlqvhlceae gipelvdvpv sfgtktlvav ktlrknateh aradflkevk ilsrlrdpni
      481 vhvlgvctrd epltmvveym engdlhqflq hhlpdtfapp iannvlsygs liymatqias
      541 gmkyleslnf vhrdlatrnc lvgssftiki adfgmsrqly acdyyriegr amlpirwmaw
      601 esvlmgtftt ksdvwafavt lweiltfarq qpfaslsdeq vienvshfyh ndglqthlpq
      661 ppncpreiyd lmrecwqrnd serpnfreih lflqrknlgy tpes
//
LOCUS       XP_077505028             698 aa            linear   INV 25-NOV-2025
DEFINITION  discoidin domain-containing receptor 2 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505028
VERSION     XP_077505028.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648902.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..698
                     /product="discoidin domain-containing receptor 2 isoform
                     X2"
                     /calculated_mol_wt=78227
     Region          398..689
                     /region_name="PTKc_DDR"
                     /note="Catalytic domain of the Protein Tyrosine Kinases,
                     Discoidin Domain Receptors; cd05051"
                     /db_xref="CDD:270644"
     Site            order(410..414,418,443,445,491..494,497..498,548,552..553,
                     555,566,584..588,597,632)
                     /site_type="active"
                     /db_xref="CDD:270644"
     Site            order(410..411,413..414,418,443,445,491..494,497..498,
                     552..553,555,566)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270644"
     Site            order(548,552,584..588,597,632)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270644"
     Site            565..590
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270644"
     CDS             1..698
                     /gene="Ddr"
                     /coded_by="XM_077648902.1:23..2119"
                     /db_xref="GeneID:144114891"
ORIGIN      
        1 malaegilay sipqgerrgt dvdlsdatyd gikddsylhg glgqltdgva gsenfkldvs
       61 gygkgyewvg wrndslhsqp lelvfefdtv rnfsavflhc nnmhtrdvqp fraarisfsv
      121 ggkhylgepq hfnlmpglfn ektrnvtirl hhhvgrfvkl qfefaakwll lsevsftsvp
      181 avgnfteeea paappivsta msedpmskqy vglvigvlaa villlvlvif vivarnrrrk
      241 hstphailkp ldsrvtinmk dlalayspqy vareangnly gqvaledpdp dkllyhepqd
      301 fkpiypgsys nesttsreya vpdmnkspvl sshksspfpq skpppppaae qyyaatdvvv
      361 slpniqgvsg ntvysvptld lssadensgg stlppvrevp rhrirfiekl gegqfgevhl
      421 ceaegipelv dvpvsfgtkt lvavktlrkn ateharadfl kevkilsrlr dpnivhvlgv
      481 ctrdepltmv veymengdlh qflqhhlpdt fappiannvl sygsliymat qiasgmkyle
      541 slnfvhrdla trnclvgssf tikiadfgms rqlyacdyyr iegramlpir wmawesvlmg
      601 tfttksdvwa favtlweilt farqqpfasl sdeqvienvs hfyhndglqt hlpqppncpr
      661 eiydlmrecw qrndserpnf reihlflqrk nlgytpes
//
LOCUS       XP_077505029             682 aa            linear   INV 25-NOV-2025
DEFINITION  discoidin domain-containing receptor 2 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505029
VERSION     XP_077505029.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648903.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..682
                     /product="discoidin domain-containing receptor 2 isoform
                     X3"
                     /calculated_mol_wt=76456
     Region          376..673
                     /region_name="PTKc_DDR"
                     /note="Catalytic domain of the Protein Tyrosine Kinases,
                     Discoidin Domain Receptors; cd05051"
                     /db_xref="CDD:270644"
     Site            order(388..392,396,427,429,475..478,481..482,532,536..537,
                     539,550,568..572,581,616)
                     /site_type="active"
                     /db_xref="CDD:270644"
     Site            order(388..389,391..392,396,427,429,475..478,481..482,
                     536..537,539,550)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270644"
     Site            order(532,536,568..572,581,616)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270644"
     Site            549..574
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270644"
     CDS             1..682
                     /gene="Ddr"
                     /coded_by="XM_077648903.1:23..2071"
                     /db_xref="GeneID:144114891"
ORIGIN      
        1 malaegilay sipqgerrgt dvdlsdatyd gikddsylhg glgqltdgva gsenfkldvs
       61 gygkgyewvg wrndslhsqp lelvfefdtv rnfsavflhc nnmhtrdvqp fraarisfsv
      121 ggkhylgepq hfnlmpglfn ektrnvtirl hhhvgrfvkl qfefaakwll lsevsftsvp
      181 avgnfteeea paappivsta msedpmskqy vglvigvlaa villlvlvif vivarnrrrk
      241 hstphailkp ldsrvtinmk valedpdpdk llyhepqdfk piypgsysne sttsreyavp
      301 dmnkspvlss hksspfpqsk pppppaaeqy yaatdvvvsl pniqgvsgnt vysvptldls
      361 sadensggst lppvrevprh rirfieklge gqfgevgarl qvhlceaegi pelvdvpvsf
      421 gtktlvavkt lrknatehar adflkevkil srlrdpnivh vlgvctrdep ltmvveymen
      481 gdlhqflqhh lpdtfappia nnvlsygsli ymatqiasgm kyleslnfvh rdlatrnclv
      541 gssftikiad fgmsrqlyac dyyriegram lpirwmawes vlmgtfttks dvwafavtlw
      601 eiltfarqqp faslsdeqvi envshfyhnd glqthlpqpp ncpreiydlm recwqrndse
      661 rpnfreihlf lqrknlgytp es
//
LOCUS       XP_077505031             676 aa            linear   INV 25-NOV-2025
DEFINITION  discoidin domain-containing receptor 2 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505031
VERSION     XP_077505031.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648905.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..676
                     /product="discoidin domain-containing receptor 2 isoform
                     X4"
                     /calculated_mol_wt=75832
     Region          376..667
                     /region_name="PTKc_DDR"
                     /note="Catalytic domain of the Protein Tyrosine Kinases,
                     Discoidin Domain Receptors; cd05051"
                     /db_xref="CDD:270644"
     Site            order(388..392,396,421,423,469..472,475..476,526,530..531,
                     533,544,562..566,575,610)
                     /site_type="active"
                     /db_xref="CDD:270644"
     Site            order(388..389,391..392,396,421,423,469..472,475..476,
                     530..531,533,544)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270644"
     Site            order(526,530,562..566,575,610)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270644"
     Site            543..568
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270644"
     CDS             1..676
                     /gene="Ddr"
                     /coded_by="XM_077648905.1:23..2053"
                     /db_xref="GeneID:144114891"
ORIGIN      
        1 malaegilay sipqgerrgt dvdlsdatyd gikddsylhg glgqltdgva gsenfkldvs
       61 gygkgyewvg wrndslhsqp lelvfefdtv rnfsavflhc nnmhtrdvqp fraarisfsv
      121 ggkhylgepq hfnlmpglfn ektrnvtirl hhhvgrfvkl qfefaakwll lsevsftsvp
      181 avgnfteeea paappivsta msedpmskqy vglvigvlaa villlvlvif vivarnrrrk
      241 hstphailkp ldsrvtinmk valedpdpdk llyhepqdfk piypgsysne sttsreyavp
      301 dmnkspvlss hksspfpqsk pppppaaeqy yaatdvvvsl pniqgvsgnt vysvptldls
      361 sadensggst lppvrevprh rirfieklge gqfgevhlce aegipelvdv pvsfgtktlv
      421 avktlrknat eharadflke vkilsrlrdp nivhvlgvct rdepltmvve ymengdlhqf
      481 lqhhlpdtfa ppiannvlsy gsliymatqi asgmkylesl nfvhrdlatr nclvgssfti
      541 kiadfgmsrq lyacdyyrie gramlpirwm awesvlmgtf ttksdvwafa vtlweiltfa
      601 rqqpfaslsd eqvienvshf yhndglqthl pqppncprei ydlmrecwqr ndserpnfre
      661 ihlflqrknl gytpes
//
LOCUS       XP_077505032             520 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114892 [Amblyomma americanum].
ACCESSION   XP_077505032
VERSION     XP_077505032.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648906.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..520
                     /product="uncharacterized protein LOC144114892"
                     /calculated_mol_wt=57796
     Region          301..322
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          323..352
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          353..375
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          376..406
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          407..434
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          435..462
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          463..485
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     CDS             1..520
                     /gene="LOC144114892"
                     /coded_by="XM_077648906.1:480..2042"
                     /db_xref="GeneID:144114892"
ORIGIN      
        1 mappvpknga dsadeassss rprivlpwsl rqqcldevgr svwrwsksvd eesepgtpvt
       61 plaenpfadc plhvrqdlle cilddearsp avrrvaelll vcgvesfdls clddcstadl
      121 qfilsmlqeh gaslcqltis glwmyspgse eivqrilksa hnlrvlriqd iyrdtlevlv
      181 rscpllqrle vmhysinaed iasvsqivqs eglgiqrslv evrlpssvng egviklietf
      241 pnlqivrcvy leavldslda rassgrrdww vrrlsrihal qsgqsmgsgs verlvawfpa
      301 leevvlhvqe dmnlrelakl irlrslelrn sptipssytd dvlpllalcg krllrlgleh
      361 fdvvdlartd imcpelesls lrwfillgca spavssraav arpfqnlrel rlrprvgrtv
      421 preacellls hcqnirhmel fcctgltdsl aavlskrngf aslksvrlrh ghglsanglq
      481 cflsraaklk fwnagkitrr sslsdddeei daagdnagdr
//
LOCUS       XP_077505033             348 aa            linear   INV 25-NOV-2025
DEFINITION  adenosine kinase 2 [Amblyomma americanum].
ACCESSION   XP_077505033
VERSION     XP_077505033.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648907.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..348
                     /product="adenosine kinase 2"
                     /calculated_mol_wt=38209
     Region          13..340
                     /region_name="ribokinase_pfkB_like"
                     /note="ribokinase/pfkB superfamily: Kinases that accept a
                     wide variety of substrates, including carbohydrates and
                     aromatic small molecules, all are phosphorylated at a
                     hydroxyl group. The superfamily includes ribokinase,
                     fructokinase, ketohexokinase; cl00192"
                     /db_xref="CDD:469648"
     Site            order(15,17,19,39,64..66,69,123,136,138,140,171,202,
                     297..298,301)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:238573"
     Site            order(266,268..269,272,285,287,293,296,299..300,303,328,
                     332)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:238573"
     CDS             1..348
                     /gene="Adk2"
                     /coded_by="XM_077648907.1:204..1250"
                     /db_xref="GeneID:144114893"
ORIGIN      
        1 mssklrkgml fgmgnplldi sasvdseflq kyslkannai ladeshtsly telvekfdcn
       61 ytaggatqns lrvfqwvvqi pevatfigci grdkfggile qkareagvnv ryqysdkent
      121 gtcavlltnh gksrslcanl aaaqlysvdh lhkpenkalm eeashyyisg fflnvslesi
      181 ltvakhacsn kkvfcmnlsa pflcrifkdn mmaafpyvdi ifgneteare fadvhnmktk
      241 diteiaklis kfpkenkefe rmviitqgad dvivaqghst qnfpvpkles daivdtngag
      301 dafvggflam yllgkpvetc ircgitvsve vvknsgctlp dresihvv
//
LOCUS       XP_077505034             342 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114894 [Amblyomma americanum].
ACCESSION   XP_077505034
VERSION     XP_077505034.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648908.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..342
                     /product="uncharacterized protein LOC144114894"
                     /calculated_mol_wt=38049
     Region          5..>81
                     /region_name="CC_brat-like"
                     /note="coiled-coil (CC) domain of Drosophila brain tumor
                     (brat) and similar proteins; cl29238"
                     /db_xref="CDD:475168"
     CDS             1..342
                     /gene="LOC144114894"
                     /coded_by="XM_077648908.1:524..1552"
                     /db_xref="GeneID:144114894"
ORIGIN      
        1 mdftetvrkk vqkaqelidg ietsrrclqk ncetlrddvd macnqqiati chrkdqlwrq
       61 idlltaeqer qlesdlarlh qyqgsllsll qlytagtlkn dcnafaclqd lelpnvttvl
      121 pqlhfsktge teleqaarrf gsaklqhacm pgmsckepak gsdsneswll cdmdncsged
      181 evvaaslpks lsatsssiea vsldeedtpg tfsklaadhp ksshitdvqq dqspwllass
      241 gyvgnseifk ltralseist tdtknheevd kanwlllprd svacrndsli smtsdnsewl
      301 lsgkaspmlt trsytmkird mfkpyfdhgn nsiwvacgrn mp
//
LOCUS       XP_077505035             531 aa            linear   INV 25-NOV-2025
DEFINITION  puff-specific protein Bx42 [Amblyomma americanum].
ACCESSION   XP_077505035
VERSION     XP_077505035.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648909.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..531
                     /product="puff-specific protein Bx42"
                     /calculated_mol_wt=60781
     Region          180..336
                     /region_name="SKIP_SNW"
                     /note="SKIP/SNW domain; pfam02731"
                     /db_xref="CDD:460666"
     CDS             1..531
                     /gene="Bx42"
                     /coded_by="XM_077648909.1:37..1632"
                     /db_xref="GeneID:144114895"
ORIGIN      
        1 maslqtllpa ptqsvfdred qdrarkyaap sthlalttrs appygmrkgw vprsiedfgd
       61 ggafpeipva qypmdmgkkk ekttsnalpv qidaqgkiky dvivrqgqsk dkviyhryqd
      121 mlpseittdd dptlqkpdee eiekttedtr lalekltqsk isaampvraa ektapaqyir
      181 ytpsqqgmsf nsgakqriir mvevqkdpme pprfkinkki prgppsppap vmhsptrkvt
      241 vkeqqewrip pcisnwknak gytvpldkrl aadgrglqqv hinenfakla ealyiadrka
      301 reavemraql ekklaqkeke kkeehlrqla qrareeragi rtqaaaaavd edvrerdqmr
      361 yerhkdrqre rniaraapdk rsklekqrer diseqialgm pgarpaevqf dqrlfnqskg
      421 lssgfghdda ydvydkpwrs aqpgvyrpkn qdkevygedl eklmktsrfv pdkefsgtdr
      481 ssrrdgpvqf erleeedpfg ldkflteakk askrptedqd hhrdkgkrrk d
//
LOCUS       XP_077505036             303 aa            linear   INV 25-NOV-2025
DEFINITION  protein phosphatase V [Amblyomma americanum].
ACCESSION   XP_077505036
VERSION     XP_077505036.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648910.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..303
                     /product="protein phosphatase V"
                     /calculated_mol_wt=34693
     Region          3..287
                     /region_name="MPP_PP2A_PP4_PP6"
                     /note="PP2A, PP4, and PP6 phosphoprotein phosphatases,
                     metallophosphatase domain; cd07415"
                     /db_xref="CDD:277360"
     Site            order(45,61,64..65,67..68,70,72..73,85,100..104,115..116,
                     119..120,124..125,128..129,137,262,271,281,287)
                     /site_type="other"
                     /note="heterotrimer interface [polypeptide binding]"
                     /db_xref="CDD:277360"
     Site            order(51,53,79,83,111..112,116..117,121,161,183..185,194,
                     207..209,235,237,261..263)
                     /site_type="active"
                     /db_xref="CDD:277360"
     Site            order(51,53,79,111,161,235)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:277360"
     CDS             1..303
                     /gene="PpV"
                     /coded_by="XM_077648910.1:84..995"
                     /db_xref="GeneID:144114896"
ORIGIN      
        1 mldvdkwidi akeckylpen dlkklcgivc rllleecniq pvstpvtvcg dihgqfydle
       61 elfrtggqvp etnyiflgdf vdrgyyslet ftrlltlkak wpdritllrg nhesrqitqv
      121 ygfydecqtk ygnanawkyc ckvfdlltva aiideqvlcv hgglspqikt ldqvrtiern
      181 qeiphkgafc dlvwsdpedv dtwsvsprga gwlfgakvth efmhlnnlkl icrahqlvhe
      241 gykymfddkl vtvwsapnyc yrcgniaavl aftdvntrta klyhavpdte rviptrnttp
      301 yfl
//
LOCUS       XP_077505037             829 aa            linear   INV 25-NOV-2025
DEFINITION  FIGNL1-interacting regulator of recombination and mitosis-like
            [Amblyomma americanum].
ACCESSION   XP_077505037
VERSION     XP_077505037.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648911.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..829
                     /product="FIGNL1-interacting regulator of recombination
                     and mitosis-like"
                     /calculated_mol_wt=92063
     Region          <344..713
                     /region_name="DUF4487"
                     /note="Domain of unknown function (DUF4487); pfam14868"
                     /db_xref="CDD:464352"
     CDS             1..829
                     /gene="LOC144114898"
                     /coded_by="XM_077648911.1:176..2665"
                     /db_xref="GeneID:144114898"
ORIGIN      
        1 mseenqdrlk qnlsaltasa engsdeqsyv gllrdicilg agepvagctv ankilsivrk
       61 kfdstcqrlv ekreascgsd dkelfnllev avtsvesaka lfericfddd akiegilval
      121 slgldllhss yahlkeksly gehlrsveda lsnlsshtvh lqtvllqcle klhvgeshek
      181 qltslssvce klsqlallle gldlkmlisa rkilvktllq yrdglkhkfs idsavsdlcr
      241 yvtewllele kmeqrkegsf lvemkavafh lrlldslllk yegyysssmk nvvkmivils
      301 rlpalevyaa nlvashrssl mlhvwiaaeh lvavladntd fcgavlgfdl cenfspgnll
      361 llttildhiv nasdmwlqpp sqtnildlif scidkcivel qcpvfleyst gdgkaprkig
      421 lyenvcihmc rfvatlpaky fpalehtlla nvfseshwra flaadvwcfv arygspqlcy
      481 ehvqllvrla klipgkhvka sahvkqllar lfdfmadehk alliqlhskd lqvlsslpls
      541 kvmptakana mqllnkflck titslelktl ehflqhlkts skaksdhlpv sillkalkti
      601 pydqslicst lteclisliv sqvahlnass igdvlrvies lhasgvdslq titalhlssf
      661 gamrnlsgsq kaeacerlar sfalvlssen yivqhiamvs faqfaqvtey ntvitkavec
      721 pslkqavtsf llkedlqkhf cwpsfvscqe kmlscswkga tkdsrgnsas hvteevipek
      781 rrkcedetek liatienava kllhaeniga mkprlsrlqe qmnailnsl
//
LOCUS       XP_077505038             667 aa            linear   INV 25-NOV-2025
DEFINITION  pumilio homolog 3-like [Amblyomma americanum].
ACCESSION   XP_077505038
VERSION     XP_077505038.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648912.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..667
                     /product="pumilio homolog 3-like"
                     /calculated_mol_wt=74336
     Region          181..>437
                     /region_name="Pumilio"
                     /note="Pumilio-family RNA binding domain; cl46378"
                     /db_xref="CDD:480718"
     Region          453..587
                     /region_name="Pumilio"
                     /note="Pumilio-family RNA binding domain; cl46378"
                     /db_xref="CDD:480718"
     CDS             1..667
                     /gene="LOC144114899"
                     /coded_by="XM_077648912.1:97..2100"
                     /db_xref="GeneID:144114899"
ORIGIN      
        1 mktkkgemkv nelsskspnr tkipkqkhkd aeakseqspk tyqagdkmkg kyakgnvrkt
       61 fppaagklgk ygklgkpkpk kpqaandims svigtqaata elsaerpfgv aasrkrglev
      121 dnedtaepkk iklvemkkkq rkqerkknes kiyeiskaak riweelrcrt ctpvrreell
      181 aqltnvvrgn ikqlifahdt srviecmehl gtavhrnmif devkdiivpm tkskyakfmv
      241 rqilrngtse qkqhvikafs thvvtllhha dasailevvy neyanalqrs wllqefysrd
      301 lalfkkdkvi tfadalaesa drarmienlk etlmkiigkp virhsiihhv mleffknada
      361 ssrsemihal gaslvemmht kdgsrvamqc iwhssakdrk tiiksfktyv akiareehgh
      421 mvllsifdcv ddtkliegai iaellkepld vlidphgqrv laylvaprdg rifhpqvvei
      481 lkegdsstts kkdpevrkqq lhkaaagalg saisdkieel mtcggptpva lhviltslst
      541 teaapayksi aklldaspym vgkdqgthif dqaparfflk klaahdkdig dkgqdsfcgv
      601 iaeevsqetm stwigcnfga fflvqlletg itkavarvka alegkhsilk ksdlkgaeil
      661 rrklhev
//
LOCUS       XP_077505039             385 aa            linear   INV 25-NOV-2025
DEFINITION  torsin-1A-interacting protein 2-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505039
VERSION     XP_077505039.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648913.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..385
                     /product="torsin-1A-interacting protein 2-like isoform X1"
                     /calculated_mol_wt=43401
     Region          183..376
                     /region_name="LAP1_C"
                     /note="Lamina-associated polypeptide 1, AAA+ activator
                     domain; pfam05609"
                     /db_xref="CDD:461691"
     CDS             1..385
                     /gene="LOC144114900"
                     /coded_by="XM_077648913.1:16..1173"
                     /db_xref="GeneID:144114900"
ORIGIN      
        1 mniafrfhly qpdikaralk phfiglrvqq kqsleamsed ddlvrsgihd rdkiptggfn
       61 tekvsdesgd eldraqhiss dtasieppgq elpacrpsgt kpaypgqrre dikkrpyerk
      121 siprspspaq lkkeappqrq qtyyifsaaa fvcglillvq ylfnetsapv pladgrlltl
      181 rehlrslkdt ytaqpeyswq iirssvvdml ndtglyagpa vitflasvkn erfatclskk
      241 vatalsytfd dggdygtida dellvsedva rhiidnrcke vvekrqrhvm vashleqwnp
      301 daammlhplc dqenaryknv tyiltvftkh edvsgkrert eydrlaetal nnawesmdsn
      361 qrfaiisrvt gnvvlvhedp escfa
//
LOCUS       XP_077505040             349 aa            linear   INV 25-NOV-2025
DEFINITION  torsin-1A-interacting protein 2-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505040
VERSION     XP_077505040.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648914.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..349
                     /product="torsin-1A-interacting protein 2-like isoform X2"
                     /calculated_mol_wt=39153
     Region          147..340
                     /region_name="LAP1_C"
                     /note="Lamina-associated polypeptide 1, AAA+ activator
                     domain; pfam05609"
                     /db_xref="CDD:461691"
     CDS             1..349
                     /gene="LOC144114900"
                     /coded_by="XM_077648914.1:3089..4138"
                     /db_xref="GeneID:144114900"
ORIGIN      
        1 msedddlvrs gihdrdkipt ggfntekvsd esgdeldraq hissdtasie ppgqelpacr
       61 psgtkpaypg qrredikkrp yerksiprsp spaqlkkeap pqrqqtyyif saaafvcgli
      121 llvqylfnet sapvpladgr lltlrehlrs lkdtytaqpe yswqiirssv vdmlndtgly
      181 agpavitfla svknerfatc lskkvatals ytfddggdyg tidadellvs edvarhiidn
      241 rckevvekrq rhvmvashle qwnpdaamml hplcdqenar yknvtyiltv ftkhedvsgk
      301 rerteydrla etalnnawes mdsnqrfaii srvtgnvvlv hedpescfa
//
LOCUS       XP_077505041             412 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114901 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505041
VERSION     XP_077505041.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648915.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..412
                     /product="uncharacterized protein LOC144114901 isoform X1"
                     /calculated_mol_wt=46978
     CDS             1..412
                     /gene="LOC144114901"
                     /coded_by="XM_077648915.1:178..1416"
                     /db_xref="GeneID:144114901"
ORIGIN      
        1 mwlllmrsct sqddfrmkrg lvsscidlfv sfgkiaamel eqrisnwlpa ldkeiveltv
       61 qklqqcgvvs lehlkyvvee dlqflkpisr rillerfhsa atpnppvsls pndiqsptst
      121 qcasfstpwe vfppqlmkac qegkrpvksd lnemvrlvsd hilainrkpg rkilraiaae
      181 ivshypktfe dtlngavigl gietllwkle ncvnnkrrps seqphqfepd deldfsvkrv
      241 kiqrdsygcv awqpklpsee tadsqekkkd dllsqfrlaf pdetmvaqlm setyasqrql
      301 inasknirdi erswpflfqa khltnhvnil lgsnvaktfd drlktvgrqv fryahsqvkk
      361 ecvksclqei eaaktnrksm aveygampll llaifrrqra vlqanrgdgq rr
//
LOCUS       XP_077505042             269 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114901 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505042
VERSION     XP_077505042.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648916.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..269
                     /product="uncharacterized protein LOC144114901 isoform X2"
                     /calculated_mol_wt=30081
     CDS             1..269
                     /gene="LOC144114901"
                     /coded_by="XM_077648916.1:175..984"
                     /db_xref="GeneID:144114901"
ORIGIN      
        1 mwlllmrsct sqddfrmkrg lvsscidlfv sfgkiaamel eqrisnwlpa ldkeiveltv
       61 qklqqcgvvs lehlkyvvee dlqflkpisr rillerfhsa atpnppvsls pndiqsptst
      121 qcasfstpwe vfppqlmkac qegkrpvksd lnememasdd dlgdlpscpf icvkgwtflt
      181 ansyticvdt hpvlhaskpd eafkllffah fafniqyqke tslcleftqr aiaginparg
      241 tkvqknggkq lclsprvaal atalidydf
//
LOCUS       XP_077505043            1089 aa            linear   INV 25-NOV-2025
DEFINITION  guanine nucleotide-releasing factor 2-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505043
VERSION     XP_077505043.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648917.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1089
                     /product="guanine nucleotide-releasing factor 2-like
                     isoform X1"
                     /calculated_mol_wt=120579
     Region          691..>765
                     /region_name="RasGEF_N"
                     /note="RasGEF N-terminal motif; pfam00618"
                     /db_xref="CDD:459873"
     Region          851..1074
                     /region_name="RasGEF"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     small GTPases. Small GTP-binding proteins of the Ras
                     superfamily function as molecular switches in fundamental
                     events such as signal transduction, cytoskeleton dynamics
                     and intracellular trafficking; cd00155"
                     /db_xref="CDD:238087"
     Site            order(882..884,894..895,897..899,901..902,905..906,909,
                     942,945..946,948..950,952..955,957..958,976,981..983,986,
                     999..1001,1004..1006,1008..1010,1012..1015,1030,1034,1068,
                     1071..1072)
                     /site_type="other"
                     /note="Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:238087"
     CDS             1..1089
                     /gene="LOC144114902"
                     /coded_by="XM_077648917.1:198..3467"
                     /db_xref="GeneID:144114902"
ORIGIN      
        1 mskkpdkhev grakgsrlar rarsfkedil gkihqmrtps raaspqqthk arprardaeq
       61 ldapatgpas pagspskgds qvnndmkqis nalryfqdsv ekntlghlpg sasivleyvl
      121 hlisllkkdl indqssvlis ttnqvyqsls rfiewaddvm lnreratmeg vnqvvqslqd
      181 slkalievsq kqsqskdknk hvgtttpngi ysskadneqh tcvchmlvds nshrnslpel
      241 pltprerqil estripdngt tvvasaapak gplchsassd silssalrpd fpppkpplph
      301 ltpprsqigg psldavappl ppkkksgals snsnllepls aalqpplfgd wqgssplsga
      361 ssfesvlhhs addllgissp tpwtttthkt syqsfsynac tttvssrsds vdqllshsss
      421 sthisamkqm dktlaslshq sfelkgsafg tamdevkpal pikqrcartr tpsqydnvsd
      481 aedvvdgerw ktehwrttdm mgckvvshep scpqyaqqts apstntssma edgrppplpp
      541 kkkmiqeyiq tfgsysqpte qqdffnsgrl ftyqvahykq vqmsfaslgs defpssslqd
      601 atlavpfels sppalppkkn rssslgvqdg lmlptrqsya slgsgtcldt pqplkaieda
      661 lpdengesli dqqdvsdhli fgdnpndvrg lkggpadali ihatavpens ekeyyyqeaf
      721 lttyktfvsp mdlvnkliyr ynkfiqfsdt rqkyarwafs llvrvvddlg lsdagpdmvr
      781 vltsfshqlv grgdlslara lrakvvekwe ahaqlhtalg gpwgstgagg appgyggsla
      841 nlrvttqpat llhfksella eqmtlldahl fqridiseil iwakeqneds spnltkfteh
      901 fnkmsywars cilkqcdake rervvvkfik imkhlrkinn fnsylailsa ldsapirrle
      961 wqkniteglk eycalidsss sfrayrqala ettppcipyi glilqdltfv hvgntdyitd
     1021 aivnftkcwq qfhilepmrc fkkkpftfkr neqiidffnn fedylsedam weisenikpr
     1081 agqkkkdys
//
LOCUS       XP_077505044            1076 aa            linear   INV 25-NOV-2025
DEFINITION  guanine nucleotide-releasing factor 2-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505044
VERSION     XP_077505044.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648918.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1076
                     /product="guanine nucleotide-releasing factor 2-like
                     isoform X2"
                     /calculated_mol_wt=119095
     Region          678..>752
                     /region_name="RasGEF_N"
                     /note="RasGEF N-terminal motif; pfam00618"
                     /db_xref="CDD:459873"
     Region          838..1061
                     /region_name="RasGEF"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     small GTPases. Small GTP-binding proteins of the Ras
                     superfamily function as molecular switches in fundamental
                     events such as signal transduction, cytoskeleton dynamics
                     and intracellular trafficking; cd00155"
                     /db_xref="CDD:238087"
     Site            order(869..871,881..882,884..886,888..889,892..893,896,
                     929,932..933,935..937,939..942,944..945,963,968..970,973,
                     986..988,991..993,995..997,999..1002,1017,1021,1055,
                     1058..1059)
                     /site_type="other"
                     /note="Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:238087"
     CDS             1..1076
                     /gene="LOC144114902"
                     /coded_by="XM_077648918.1:200..3430"
                     /db_xref="GeneID:144114902"
ORIGIN      
        1 mskkpdkhev grakgsrlar rarsfkedil gkihqmrtps raaspqqthk arprardaeq
       61 ldapatgpas pagspskgds qvnndmkqis nalryfqdsv ekntlghlpg sasivleyvl
      121 hlisllkkdl indqssvlis ttnqvyqsls rfiewaddvm lnreratmeg vnqvvqslqd
      181 slkalievsq kqsqskdknk hvgtttpngi ysskadnnsh rnslpelplt prerqilest
      241 ripdngttvv asaapakgpl chsassdsil ssalrpdfpp pkpplphltp prsqiggpsl
      301 davapplppk kksgalssns nlleplsaal qpplfgdwqg ssplsgassf esvlhhsadd
      361 llgissptpw tttthktsyq sfsynacttt vssrsdsvdq llshssssth isamkqmdkt
      421 laslshqsfe lkgsafgtam devkpalpik qrcartrtps qydnvsdaed vvdgerwkte
      481 hwrttdmmgc kvvshepscp qyaqqtsaps tntssmaedg rppplppkkk miqeyiqtfg
      541 sysqpteqqd ffnsgrlfty qvahykqvqm sfaslgsdef pssslqdatl avpfelsspp
      601 alppkknrss slgvqdglml ptrqsyaslg sgtcldtpqp lkaiedalpd engeslidqq
      661 dvsdhlifgd npndvrglkg gpadaliiha tavpenseke yyyqeafltt yktfvspmdl
      721 vnkliyrynk fiqfsdtrqk yarwafsllv rvvddlglsd agpdmvrvlt sfshqlvgrg
      781 dlslaralra kvvekweaha qlhtalggpw gstgaggapp gyggslanlr vttqpatllh
      841 fksellaeqm tlldahlfqr idiseiliwa keqnedsspn ltkftehfnk msywarscil
      901 kqcdakerer vvvkfikimk hlrkinnfns ylailsalds apirrlewqk niteglkeyc
      961 alidssssfr ayrqalaett ppcipyigli lqdltfvhvg ntdyitdaiv nftkcwqqfh
     1021 ilepmrcfkk kpftfkrneq iidffnnfed ylsedamwei senikpragq kkkdys
//
LOCUS       XP_077505045            1001 aa            linear   INV 25-NOV-2025
DEFINITION  rap guanine nucleotide exchange factor 1-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505045
VERSION     XP_077505045.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648919.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1001
                     /product="rap guanine nucleotide exchange factor 1-like
                     isoform X3"
                     /calculated_mol_wt=111121
     Region          603..>677
                     /region_name="RasGEF_N"
                     /note="RasGEF N-terminal motif; pfam00618"
                     /db_xref="CDD:459873"
     Region          763..986
                     /region_name="RasGEF"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     small GTPases. Small GTP-binding proteins of the Ras
                     superfamily function as molecular switches in fundamental
                     events such as signal transduction, cytoskeleton dynamics
                     and intracellular trafficking; cd00155"
                     /db_xref="CDD:238087"
     Site            order(794..796,806..807,809..811,813..814,817..818,821,
                     854,857..858,860..862,864..867,869..870,888,893..895,898,
                     911..913,916..918,920..922,924..927,942,946,980,983..984)
                     /site_type="other"
                     /note="Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:238087"
     CDS             1..1001
                     /gene="LOC144114902"
                     /coded_by="XM_077648919.1:204..3209"
                     /db_xref="GeneID:144114902"
ORIGIN      
        1 mskkpdkhev grakgsrlar rarsfkedil gkihqmrtps raaspqqthk arprardaeq
       61 ldapatgpas pagspskgds qvnndmkqis nalryfqdsv ekntlghlpg sasivleyvl
      121 hlisllkkdl indqssvlis ttnqvyqsls rfiewaddvm lnreratmeg vnqvvqslqd
      181 slkalievsq kqsqskdknk hvgtttpngi ysskadksqi ggpsldavap plppkkksga
      241 lssnsnllep lsaalqpplf gdwqgsspls gassfesvlh hsaddllgis sptpwtttth
      301 ktsyqsfsyn actttvssrs dsvdqllshs sssthisamk qmdktlasls hqsfelkgsa
      361 fgtamdevkp alpikqrcar trtpsqydnv sdaedvvdge rwktehwrtt dmmgckvvsh
      421 epscpqyaqq tsapstntss maedgrpppl ppkkkmiqey iqtfgsysqp teqqdffnsg
      481 rlftyqvahy kqvqmsfasl gsdefpsssl qdatlavpfe lssppalppk knrssslgvq
      541 dglmlptrqs yaslgsgtcl dtpqplkaie dalpdenges lidqqdvsdh lifgdnpndv
      601 rglkggpada liihatavpe nsekeyyyqe aflttyktfv spmdlvnkli yrynkfiqfs
      661 dtrqkyarwa fsllvrvvdd lglsdagpdm vrvltsfshq lvgrgdlsla ralrakvvek
      721 weahaqlhta lggpwgstga ggappgyggs lanlrvttqp atllhfksel laeqmtllda
      781 hlfqridise iliwakeqne dsspnltkft ehfnkmsywa rscilkqcda kerervvvkf
      841 ikimkhlrki nnfnsylail saldsapirr lewqkniteg lkeycalids sssfrayrqa
      901 laettppcip yiglilqdlt fvhvgntdyi tdaivnftkc wqqfhilepm rcfkkkpftf
      961 krneqiidff nnfedylsed amweisenik pragqkkkdy s
//
LOCUS       XP_077505046             246 aa            linear   INV 25-NOV-2025
DEFINITION  dolichyldiphosphatase 1-like [Amblyomma americanum].
ACCESSION   XP_077505046
VERSION     XP_077505046.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648920.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..246
                     /product="dolichyldiphosphatase 1-like"
                     /calculated_mol_wt=28256
     Region          25..187
                     /region_name="PAP2_dolichyldiphosphatase"
                     /note="PAP2_like proteins, dolichyldiphosphatase
                     subfamily. Dolichyldiphosphatase is a membrane-associated
                     protein located in the endoplasmic reticulum and
                     hydrolyzes dolichyl pyrophosphate, as well as
                     dolichylmonophosphate at a low rate. The enzyme is...;
                     cd03382"
                     /db_xref="CDD:239477"
     Site            order(86,93,108..110,161,167,171)
                     /site_type="active"
                     /db_xref="CDD:239477"
     CDS             1..246
                     /gene="LOC144114903"
                     /coded_by="XM_077648920.1:50..790"
                     /db_xref="GeneID:144114903"
ORIGIN      
        1 maggrpgngp vpgdfdneir swvplslthv eypagdwfgk tlayislspl vilvsfgtlv
       61 ifrrdlhtit flcgtllsea infvlkhvik earpykardn ftefgmpssh sqlmwfvaty
      121 laffvivrlh hgnsscpwen lwkytvivcw fllagtvsys rvyleyhtwa qvcwgaligs
      181 vlaclwfcit qfiltplypr ivswhlsell mlrdttlipn vmwfeytsyr aesrtrqrkl
      241 vsmksq
//
LOCUS       XP_077505047             396 aa            linear   INV 25-NOV-2025
DEFINITION  tRNA-guanine transglycosylase [Amblyomma americanum].
ACCESSION   XP_077505047
VERSION     XP_077505047.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648921.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..396
                     /product="tRNA-guanine transglycosylase"
                     /calculated_mol_wt=43825
     Region          21..375
                     /region_name="TGT"
                     /note="Queuine tRNA-ribosyltransferase; pfam01702"
                     /db_xref="CDD:460299"
     CDS             1..396
                     /gene="Tgt"
                     /coded_by="XM_077648921.1:26..1216"
                     /db_xref="GeneID:144114905"
ORIGIN      
        1 maaplkhalk feivaecnvt karaslltlp havvetpvfm pvgtqgtlkg lvpeqieeqk
       61 cnmilantyh lgmrpgtdil qkagglhkfm nwknglltds ggfqmvslvk lsevteegvr
      121 fvspydqrei mlspekstei qntihadimm qlddvvssti sgprveeamh rtirwldrcm
      181 aahknsatqn ifpivqggld aalrercale llkrdvagya igglsggesk ddfwpmvdis
      241 tnllpknkpr ylmgvgfavd lvvcsalgcd mfdcvfptrt arfgcalvmg gqlnlkntef
      301 cndfspiedd cpcstcrqyt raylhhivkq etvachlvsv hnvnfqmrlm ksirdnikag
      361 tfvsfvkafm ktyypksdyp gwvvdalaav nihldl
//
LOCUS       XP_077505048            2394 aa            linear   INV 25-NOV-2025
DEFINITION  neurogenic locus Notch protein [Amblyomma americanum].
ACCESSION   XP_077505048
VERSION     XP_077505048.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648922.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2394
                     /product="neurogenic locus Notch protein"
                     /calculated_mol_wt=256010
     Region          152..186
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          189..224
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(189,192,205)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          231..263
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          265..301
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(265,268,282)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          303..340
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(303,306,322)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          343..378
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(343,346,360)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          420..457
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(420,423,438)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          459..495
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(459,462,476)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          497..533
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(497,500,514)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          535..571
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(535,538,552)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          573..608
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(573,576,589)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          611..646
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          648..684
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(648,651,665)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          686..721
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(686,689,703)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          724..760
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(724,727,741)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          762..798
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(762,765,779)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          800..835
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(800,803,817)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          878..914
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(878,881,896)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          917..952
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(917,920,934)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          955..991
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(955,958,972)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          996..1029
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          1032..1067
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          <1077..1104
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          <1124..1153
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          1156..1191
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          1193..1229
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(1193,1196,1210)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          1231..1267
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(1231,1234,1248)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          1269..1307
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(1269,1272,1288)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          1313..1343
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     Region          1452..1485
                     /region_name="Notch"
                     /note="LNR domain; pfam00066"
                     /db_xref="CDD:459658"
     Region          1491..1526
                     /region_name="Notch"
                     /note="LNR domain; pfam00066"
                     /db_xref="CDD:459658"
     Region          1539..1566
                     /region_name="Notch"
                     /note="LNR domain; pfam00066"
                     /db_xref="CDD:459658"
     Region          1569..1623
                     /region_name="NOD"
                     /note="NOTCH protein; pfam06816"
                     /db_xref="CDD:462014"
     Region          1640..1691
                     /region_name="NODP"
                     /note="NOTCH protein; pfam07684"
                     /db_xref="CDD:462229"
     Region          <1727..1769
                     /region_name="JMTM_Notch_APP"
                     /note="juxtamembrane and transmembrane (JMTM) domain found
                     in Notch and APP family proteins; cl41775"
                     /db_xref="CDD:425406"
     Region          1858..1903
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1886..2095
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          1906..1937
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1939..1971
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1973,1975,1979..1980,1983..1985,1987..1988,1992,
                     1995,2004,2006,2008,2012..2013,2016..2018,2020..2021,2025,
                     2028,2037,2039,2041,2045..2046,2049..2051,2053..2054,2058,
                     2061,2070)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1973..2004
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          2006..2037
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          2039..2070
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          2072..2101
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          2152..>2391
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     CDS             1..2394
                     /gene="N"
                     /coded_by="XM_077648922.1:265..7449"
                     /db_xref="GeneID:144114907"
ORIGIN      
        1 mkprktgvav glwlalvaat ipltvqafvs cspnpcknga scvssnpsge shcncpplyv
       61 geycqhqnpc htgpgfrcqn ggtcnvemsv tngpkfsctc pvgytatmce ikvpnacdsq
      121 pchngatcql ltlqnytcvc angfrgkycd qvdhcasqpc lnygscqpke dsfsctcapg
      181 fsgascavdv decqarpcmh gtclntqgsf scvceagytg qrcesqyipc epspcmnggt
      241 crpidslnyq cscppgftgt ncetnvddcp gnlcqngatc ldgvnsytch cpatytgpyc
      301 tkdvdecalr psvckngatc tntvggysci cvngwtgadc seniddcava acfngatchd
      361 rvgsfycqca pgktgllchl ddacasnpch agaicdtspi dgtylcscpn gfqgvdcted
      421 vdecaqgfpc ehqglcvntp gsfrcnctrg fagprcevni necdsnpcqn egtcldergg
      481 frcvcmpgyr gvhcevdide clpnpclngg icsdringfq clcpvgfsgk kcetneddcs
      541 sfpcrnggsc hdgiasytch cppgftgstc etnmddcqss pchhgtcidg tnaftcnchp
      601 gytgllcqnq ineclsspcq hggtcedlvn gyqcrcrrgt sgtdcqfnvn ecfsnpcrng
      661 aacvdgidsy tceclpgytg ihcetdinec asnpcangga cidlvnsfkc dcprgyydar
      721 clsdvnecas npcqhggtce devnhfvchc ppgygghrce qdidecqsnp cqhggscrda
      781 lnayscacie gyagrncetn lddcspnpcl nggscidlvg tfrcvcevpf tgptcdveld
      841 pcspnkcrng aqcspssnyl dfacscklgf tgrlcdedid ecavsspcrn eatcintngs
      901 yecactkgye grdclintdd casypcqngg tcldgigeyt clcvdgfgge hcsidideca
      961 snpclngatc ndyvnsyaca clpgfsgtnc qtndedctss scmnggtcvd gvnnytcqcs
     1021 pgytgsncqy hinecdsqpc ahgatcvnhi gyhtchcpfg ytgprcetlv dwcattpcln
     1081 gatckqsnnt yrclckpgwt gllcdvsmvs cedaalqkgv kvadlcqhgg acedignshr
     1141 clcpegyegs ycqrevnecl snpcqngatc hnligqytcd cpegfqglnc eynindcdpn
     1201 pchnggtchd lvnkfacscr hgtvgilceh nvnecfegac hhggtcldrv ngyececrpg
     1261 yvgprcegdv neclskpcdp vgtldcvqli ddyrcdcrpg hagrhcehkv dpcssnpcln
     1321 ggvchpgprg pmcicqegfr getcanqscg ekwpcrnggq crghscicph rttgslceii
     1381 eeelsksact lgpcrnggvc relpggriec dcparwkgpr ceeydasfrg slappvegiy
     1441 dlflksleee kkqcamrrce kkagnrycde ecntyacdfd ggdcslginp wmnctaaikc
     1501 wdvfrdgkcn mecnnidclf dgfdcqkdlq pcnehydtyc mrnygngycd qgcnneecnw
     1561 dgldcemdpp ylaegsivim lsiepevfkn ntvpflrdig halrtnvlfk tdangqamiy
     1621 pwkategqlp nhsvlpvprt gtlvhleidn rrclksqgse cfqsaakaae flaaaqarha
     1681 letpfhiqev lgsgrekggs ggegsrasal evvigliavl lavlligvlf ttqkkrargi
     1741 twfpegflst ssgqrrrsrr rgpdgqemrv aqasgngppe awsddegerp pakrargspd
     1801 sgcgdtvvsd yederdprpw tqqhldaadv rlpevlqalt ppqgsdldhn vdvrgpgglt
     1861 plmlasfrgg gvdtgeepge edtgsasmiq dllmqgaqlg sttektgets lhlaaryara
     1921 daakrlldag adanaqdssg rtplhaaiaa davgvfqill rnratnlnak mnngmtplil
     1981 sirlamegma edlinaeadv saadadsrtp lhwaaavndv asvrillahg anrdaqdvhe
     2041 etplflaare gsleavrall dhaanrditn hmdrrprdva rdrmhldive lldkyvpsph
     2101 asglssppml lssptvigst ksargkrrqp hvkngassai qqldmpatsp ktdaaaatiq
     2161 krrtpsikkc rdppalpdva hsldsphess ggplflpqea vatftkqppc yedcvgpvya
     2221 gvydlqayan tgstamsqqq qqqqqhqrqt svpssmggyg iasspakqrp slptspthma
     2281 amraahqhkl qqggptydyp lqpayyhypt ppsqhshgsg aeatpqhylh pgetyptpsp
     2341 espgqwssss phsaqsdwse gissplgqal ggsadskqqa mqqpglphpd pvfi
//
LOCUS       XP_077505049             182 aa            linear   INV 25-NOV-2025
DEFINITION  U3 small nucleolar ribonucleoprotein IMP3 [Amblyomma americanum].
ACCESSION   XP_077505049
VERSION     XP_077505049.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648923.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..182
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..182
                     /product="U3 small nucleolar ribonucleoprotein IMP3"
                     /calculated_mol_wt=21380
     Region          4..86
                     /region_name="Ribosomal_S4"
                     /note="Ribosomal protein S4/S9 N-terminal domain;
                     pfam00163"
                     /db_xref="CDD:459696"
     Region          29..166
                     /region_name="uS4_arch"
                     /note="ribosomal protein uS4, eukaryotic/archaeal type;
                     TIGR01018"
                     /db_xref="CDD:273397"
     CDS             1..182
                     /gene="LOC144114908"
                     /coded_by="XM_077648923.1:64..612"
                     /db_xref="GeneID:144114908"
ORIGIN      
        1 mvrklkfheq kllkkvdfis wevdnnlhev kimrkyyvqk redytkynkl sravrdlark
       61 ikeldpkdpf rakatsdlln klhslgviai kenleicdrv tassfcrrrl pvimvrnqma
      121 qrvkmatqfv eqghvrigpd vitdpaflvs rqledfvtwt nssaikkhva eyndmrddyd
      181 ll
//
LOCUS       XP_077505050             876 aa            linear   INV 25-NOV-2025
DEFINITION  dynamin-1 shibire isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505050
VERSION     XP_077505050.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648924.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..876
                     /product="dynamin-1 shibire isoform X1"
                     /calculated_mol_wt=97915
     Region          7..246
                     /region_name="DYNc"
                     /note="Dynamin, GTPase; smart00053"
                     /db_xref="CDD:197491"
     Site            39..46
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206738"
     Site            order(40,42..47,60..61,66,137,207,209..210,236..240,243)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206738"
     Site            order(41..42,141,154,179,181,183..186,188..189,210,
                     212..215,239..241)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:206738"
     Site            66
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206738"
     Site            70..72
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206738"
     Site            137..140
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206738"
     Site            order(139..140,170..171)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206738"
     Site            206..209
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206738"
     Region          216..500
                     /region_name="Dynamin_M"
                     /note="Dynamin central region; pfam01031"
                     /db_xref="CDD:460033"
     Site            236..238
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206738"
     Region          529..639
                     /region_name="PH_dynamin"
                     /note="Dynamin pleckstrin homology (PH) domain; cd01256"
                     /db_xref="CDD:269958"
     Site            order(529,531..532,534,551,621..622,625,628..629,632)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:269958"
     Region          667..757
                     /region_name="GED"
                     /note="Dynamin GTPase effector domain; pfam02212"
                     /db_xref="CDD:460495"
     CDS             1..876
                     /gene="shi"
                     /coded_by="XM_077648924.1:165..2795"
                     /db_xref="GeneID:144114909"
ORIGIN      
        1 msgndgmegl ipivnrlqda yaqlgssltl dlpqiavvgg qsagkssvle nfvgrdflpr
       61 gsgivtrrpl vlqlincnte ygeflhcrgk kftdfdqirk eiedetdrvt gtnkgissvp
      121 inlrvysplv lnltlvdlpg ltkvpvgdqp adieqqirdm ilqfirkenc lilavtsanq
      181 dlatsdalkl akevdpeglr tigvitkldl mdegtdardi lenkllplrr gyvgvvnrsq
      241 kdiegkkdik aameaerkff lshpayrhma drmgtpylqr vlnqqltnhi rdtlpglrdk
      301 lqkqlismek eveeyknfrp ddpsrktkam lqmiqqlqtd ferniegsgs aaintselsg
      361 garinrlfhe rfpfeivkme fdekelrkei afairnthgi rvglftpdma feaivkkqia
      421 klkepsikcv dlvvaelgnv irrcaekmsr yprlreeter iitshvrere qtakqqisll
      481 vevelaymnt nhedfigftk ddyhsgaqqt advssagkrk lgnqsvfavi rkgwmcihnl
      541 gimkggsrdy wfvltsesis wykdeeekdk kymlpldglk ikdieagfms rrntfalfnp
      601 dqrrnvykdy kqlelscesq eevdswkasf lragvypera qsaagdgegg tdgggpegsg
      661 ssmdpqlerq vetirnlvds ymriitktfr dlvpkiimhl tindtkefiy sellaalyas
      721 gdqtqlmees peeaqkrdem lrmyhackea lriigdvsmq tsyqptpppv kddwlkmqpp
      781 dsprpappsp ggprrnvgss rapppvpaaa srpapvipsr pestgrappa apprpisagf
      841 qaplvpsrgs lpsvpprvaq gavdtmldaf gmpmsk
//
LOCUS       XP_077505051             875 aa            linear   INV 25-NOV-2025
DEFINITION  dynamin-1 shibire isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505051
VERSION     XP_077505051.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648925.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..875
                     /product="dynamin-1 shibire isoform X2"
                     /calculated_mol_wt=97759
     Region          7..246
                     /region_name="DYNc"
                     /note="Dynamin, GTPase; smart00053"
                     /db_xref="CDD:197491"
     Site            39..46
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206738"
     Site            order(40,42..47,60..61,66,137,207,209..210,236..240,243)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206738"
     Site            order(41..42,141,154,179,181,183..186,188..189,210,
                     212..215,239..241)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:206738"
     Site            66
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206738"
     Site            70..72
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206738"
     Site            137..140
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206738"
     Site            order(139..140,170..171)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206738"
     Site            206..209
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206738"
     Region          216..500
                     /region_name="Dynamin_M"
                     /note="Dynamin central region; pfam01031"
                     /db_xref="CDD:460033"
     Site            236..238
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206738"
     Region          529..638
                     /region_name="PH_dynamin"
                     /note="Dynamin pleckstrin homology (PH) domain; cd01256"
                     /db_xref="CDD:269958"
     Site            order(529,531..532,534,551,620..621,624,627..628,631)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:269958"
     Region          666..756
                     /region_name="GED"
                     /note="Dynamin GTPase effector domain; pfam02212"
                     /db_xref="CDD:460495"
     CDS             1..875
                     /gene="shi"
                     /coded_by="XM_077648925.1:165..2792"
                     /db_xref="GeneID:144114909"
ORIGIN      
        1 msgndgmegl ipivnrlqda yaqlgssltl dlpqiavvgg qsagkssvle nfvgrdflpr
       61 gsgivtrrpl vlqlincnte ygeflhcrgk kftdfdqirk eiedetdrvt gtnkgissvp
      121 inlrvysplv lnltlvdlpg ltkvpvgdqp adieqqirdm ilqfirkenc lilavtsanq
      181 dlatsdalkl akevdpeglr tigvitkldl mdegtdardi lenkllplrr gyvgvvnrsq
      241 kdiegkkdik aameaerkff lshpayrhma drmgtpylqr vlnqqltnhi rdtlpglrdk
      301 lqkqlismek eveeyknfrp ddpsrktkam lqmiqqlqtd ferniegsgs aaintselsg
      361 garinrlfhe rfpfeivkme fdekelrkei afairnthgi rvglftpdma feaivkkqia
      421 klkepsikcv dlvvaelgnv irrcaekmsr yprlreeter iitshvrere qtakqqisll
      481 vevelaymnt nhedfigftk ddyhsgaqqt advssagkrk lgnqsvfavi rkgwmcihnl
      541 gimkggsrdy wfvltsesis wykdeeekdk kymlpldglk ikdieagfms rrntfalfnp
      601 dqrnvykdyk qlelscesqe evdswkasfl ragvyperaq saagdgeggt dgggpegsgs
      661 smdpqlerqv etirnlvdsy mriitktfrd lvpkiimhlt indtkefiys ellaalyasg
      721 dqtqlmeesp eeaqkrdeml rmyhackeal riigdvsmqt syqptpppvk ddwlkmqppd
      781 sprpappspg gprrnvgssr apppvpaaas rpapvipsrp estgrappaa pprpisagfq
      841 aplvpsrgsl psvpprvaqg avdtmldafg mpmsk
//
LOCUS       XP_077505052             872 aa            linear   INV 25-NOV-2025
DEFINITION  dynamin-1 shibire isoform X3 [Amblyomma americanum].
ACCESSION   XP_077505052
VERSION     XP_077505052.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648926.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..872
                     /product="dynamin-1 shibire isoform X3"
                     /calculated_mol_wt=97511
     Region          7..246
                     /region_name="DYNc"
                     /note="Dynamin, GTPase; smart00053"
                     /db_xref="CDD:197491"
     Site            39..46
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206738"
     Site            order(40,42..47,60..61,66,137,207,209..210,236..240,243)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206738"
     Site            order(41..42,141,154,179,181,183..186,188..189,210,
                     212..215,239..241)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:206738"
     Site            66
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206738"
     Site            70..72
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206738"
     Site            137..140
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206738"
     Site            order(139..140,170..171)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206738"
     Site            206..209
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206738"
     Region          216..500
                     /region_name="Dynamin_M"
                     /note="Dynamin central region; pfam01031"
                     /db_xref="CDD:460033"
     Site            236..238
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206738"
     Region          523..635
                     /region_name="PH_dynamin"
                     /note="Dynamin pleckstrin homology (PH) domain; cd01256"
                     /db_xref="CDD:269958"
     Site            order(525,527..528,530,547,617..618,621,624..625,628)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:269958"
     Region          663..753
                     /region_name="GED"
                     /note="Dynamin GTPase effector domain; pfam02212"
                     /db_xref="CDD:460495"
     CDS             1..872
                     /gene="shi"
                     /coded_by="XM_077648926.1:165..2783"
                     /db_xref="GeneID:144114909"
ORIGIN      
        1 msgndgmegl ipivnrlqda yaqlgssltl dlpqiavvgg qsagkssvle nfvgrdflpr
       61 gsgivtrrpl vlqlincnte ygeflhcrgk kftdfdqirk eiedetdrvt gtnkgissvp
      121 inlrvysplv lnltlvdlpg ltkvpvgdqp adieqqirdm ilqfirkenc lilavtsanq
      181 dlatsdalkl akevdpeglr tigvitkldl mdegtdardi lenkllplrr gyvgvvnrsq
      241 kdiegkkdik aameaerkff lshpayrhma drmgtpylqr vlnqqltnhi rdtlpglrdk
      301 lqkqlismek eveeyknfrp ddpsrktkam lqmiqqlqtd ferniegsgs aaintselsg
      361 garinrlfhe rfpfeivkme fdekelrkei afairnthgi rvglftpdma feaivkkqia
      421 klkepsikcv dlvvaelgnv irrcaekmsr yprlreeter iitshvrere qtakqqisll
      481 vevelaymnt nhedfigftk ddyhsgaqqt advssagkrk lgnqvirkgw mcihnlgimk
      541 ggsrdywfvl tsesiswykd eeekdkkyml pldglkikdi eagfmsrrnt falfnpdqrr
      601 nvykdykqle lscesqeevd swkasflrag vyperaqsaa gdgeggtdgg gpegsgssmd
      661 pqlerqveti rnlvdsymri itktfrdlvp kiimhltind tkefiysell aalyasgdqt
      721 qlmeespeea qkrdemlrmy hackealrii gdvsmqtsyq ptpppvkddw lkmqppdspr
      781 pappspggpr rnvgssrapp pvpaaasrpa pvipsrpest grappaappr pisagfqapl
      841 vpsrgslpsv pprvaqgavd tmldafgmpm sk
//
LOCUS       XP_077505053             871 aa            linear   INV 25-NOV-2025
DEFINITION  dynamin-1 shibire isoform X4 [Amblyomma americanum].
ACCESSION   XP_077505053
VERSION     XP_077505053.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648927.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..871
                     /product="dynamin-1 shibire isoform X4"
                     /calculated_mol_wt=97355
     Region          7..246
                     /region_name="DYNc"
                     /note="Dynamin, GTPase; smart00053"
                     /db_xref="CDD:197491"
     Site            39..46
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206738"
     Site            order(40,42..47,60..61,66,137,207,209..210,236..240,243)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206738"
     Site            order(41..42,141,154,179,181,183..186,188..189,210,
                     212..215,239..241)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:206738"
     Site            66
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206738"
     Site            70..72
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206738"
     Site            137..140
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206738"
     Site            order(139..140,170..171)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206738"
     Site            206..209
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206738"
     Region          216..500
                     /region_name="Dynamin_M"
                     /note="Dynamin central region; pfam01031"
                     /db_xref="CDD:460033"
     Site            236..238
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206738"
     Region          523..634
                     /region_name="PH_dynamin"
                     /note="Dynamin pleckstrin homology (PH) domain; cd01256"
                     /db_xref="CDD:269958"
     Site            order(525,527..528,530,547,616..617,620,623..624,627)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:269958"
     Region          662..752
                     /region_name="GED"
                     /note="Dynamin GTPase effector domain; pfam02212"
                     /db_xref="CDD:460495"
     CDS             1..871
                     /gene="shi"
                     /coded_by="XM_077648927.1:164..2779"
                     /db_xref="GeneID:144114909"
ORIGIN      
        1 msgndgmegl ipivnrlqda yaqlgssltl dlpqiavvgg qsagkssvle nfvgrdflpr
       61 gsgivtrrpl vlqlincnte ygeflhcrgk kftdfdqirk eiedetdrvt gtnkgissvp
      121 inlrvysplv lnltlvdlpg ltkvpvgdqp adieqqirdm ilqfirkenc lilavtsanq
      181 dlatsdalkl akevdpeglr tigvitkldl mdegtdardi lenkllplrr gyvgvvnrsq
      241 kdiegkkdik aameaerkff lshpayrhma drmgtpylqr vlnqqltnhi rdtlpglrdk
      301 lqkqlismek eveeyknfrp ddpsrktkam lqmiqqlqtd ferniegsgs aaintselsg
      361 garinrlfhe rfpfeivkme fdekelrkei afairnthgi rvglftpdma feaivkkqia
      421 klkepsikcv dlvvaelgnv irrcaekmsr yprlreeter iitshvrere qtakqqisll
      481 vevelaymnt nhedfigftk ddyhsgaqqt advssagkrk lgnqvirkgw mcihnlgimk
      541 ggsrdywfvl tsesiswykd eeekdkkyml pldglkikdi eagfmsrrnt falfnpdqrn
      601 vykdykqlel scesqeevds wkasflragv yperaqsaag dgeggtdggg pegsgssmdp
      661 qlerqvetir nlvdsymrii tktfrdlvpk iimhltindt kefiysella alyasgdqtq
      721 lmeespeeaq krdemlrmyh ackealriig dvsmqtsyqp tpppvkddwl kmqppdsprp
      781 appspggprr nvgssrappp vpaaasrpap vipsrpestg rappaapprp isagfqaplv
      841 psrgslpsvp prvaqgavdt mldafgmpms k
//
LOCUS       XP_077505054             870 aa            linear   INV 25-NOV-2025
DEFINITION  dynamin-1 shibire isoform X5 [Amblyomma americanum].
ACCESSION   XP_077505054
VERSION     XP_077505054.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648928.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..870
                     /product="dynamin-1 shibire isoform X5"
                     /calculated_mol_wt=97545
     Region          7..246
                     /region_name="DYNc"
                     /note="Dynamin, GTPase; smart00053"
                     /db_xref="CDD:197491"
     Site            39..46
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206738"
     Site            order(40,42..47,60..61,66,137,207,209..210,236..240,243)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206738"
     Site            order(41..42,141,154,179,181,183..186,188..189,210,
                     212..215,239..241)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:206738"
     Site            66
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206738"
     Site            70..72
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206738"
     Site            137..140
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206738"
     Site            order(139..140,170..171)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206738"
     Site            206..209
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206738"
     Region          216..500
                     /region_name="Dynamin_M"
                     /note="Dynamin central region; pfam01031"
                     /db_xref="CDD:460033"
     Site            236..238
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206738"
     Region          529..639
                     /region_name="PH_dynamin"
                     /note="Dynamin pleckstrin homology (PH) domain; cd01256"
                     /db_xref="CDD:269958"
     Site            order(529,531..532,534,551,621..622,625,628..629,632)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:269958"
     Region          667..757
                     /region_name="GED"
                     /note="Dynamin GTPase effector domain; pfam02212"
                     /db_xref="CDD:460495"
     CDS             1..870
                     /gene="shi"
                     /coded_by="XM_077648928.1:162..2774"
                     /db_xref="GeneID:144114909"
ORIGIN      
        1 msgndgmegl ipivnrlqda yaqlgssltl dlpqiavvgg qsagkssvle nfvgrdflpr
       61 gsgivtrrpl vlqlincnte ygeflhcrgk kftdfdqirk eiedetdrvt gtnkgissvp
      121 inlrvysplv lnltlvdlpg ltkvpvgdqp adieqqirdm ilqfirkenc lilavtsanq
      181 dlatsdalkl akevdpeglr tigvitkldl mdegtdardi lenkllplrr gyvgvvnrsq
      241 kdiegkkdik aameaerkff lshpayrhma drmgtpylqr vlnqqltnhi rdtlpglrdk
      301 lqkqlismek eveeyknfrp ddpsrktkam lqmiqqlqtd ferniegsgs aaintselsg
      361 garinrlfhe rfpfeivkme fdekelrkei afairnthgi rvglftpdma feaivkkqia
      421 klkepsikcv dlvvaelgnv irrcaekmsr yprlreeter iitshvrere qtakqqisll
      481 vevelaymnt nhedfigftk ddyhsgaqqt advssagkrk lgnqsvfavi rkgwmcihnl
      541 gimkggsrdy wfvltsesis wykdeeekdk kymlpldglk ikdieagfms rrntfalfnp
      601 dqrrnvykdy kqlelscesq eevdswkasf lragvypera qsaagdgegg tdgggpegsg
      661 ssmdpqlerq vetirnlvds ymriitktfr dlvpkiimhl tindtkefiy sellaalyas
      721 gdqtqlmees peeaqkrdem lrmyhackea lriigdvsmq tsyqptpppv kddwlkmqpp
      781 dsprpappsp ggprrnvgss rapppvpaaa srpapvipsr pestgrappa apprpisagf
      841 qaplvpsrpv psvppripdr pqvsssqwpy
//
LOCUS       XP_077505055             870 aa            linear   INV 25-NOV-2025
DEFINITION  dynamin-1 shibire isoform X6 [Amblyomma americanum].
ACCESSION   XP_077505055
VERSION     XP_077505055.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648929.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..870
                     /product="dynamin-1 shibire isoform X6"
                     /calculated_mol_wt=97227
     Region          7..246
                     /region_name="DYNc"
                     /note="Dynamin, GTPase; smart00053"
                     /db_xref="CDD:197491"
     Site            39..46
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206738"
     Site            order(40,42..47,60..61,66,137,207,209..210,236..240,243)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206738"
     Site            order(41..42,141,154,179,181,183..186,188..189,210,
                     212..215,239..241)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:206738"
     Site            66
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206738"
     Site            70..72
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206738"
     Site            137..140
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206738"
     Site            order(139..140,170..171)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206738"
     Site            206..209
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206738"
     Region          216..503
                     /region_name="Dynamin_M"
                     /note="Dynamin central region; pfam01031"
                     /db_xref="CDD:460033"
     Site            236..238
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206738"
     Region          523..633
                     /region_name="PH_dynamin"
                     /note="Dynamin pleckstrin homology (PH) domain; cd01256"
                     /db_xref="CDD:269958"
     Site            order(523,525..526,528,545,615..616,619,622..623,626)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:269958"
     Region          661..751
                     /region_name="GED"
                     /note="Dynamin GTPase effector domain; pfam02212"
                     /db_xref="CDD:460495"
     CDS             1..870
                     /gene="shi"
                     /coded_by="XM_077648929.1:164..2776"
                     /db_xref="GeneID:144114909"
ORIGIN      
        1 msgndgmegl ipivnrlqda yaqlgssltl dlpqiavvgg qsagkssvle nfvgrdflpr
       61 gsgivtrrpl vlqlincnte ygeflhcrgk kftdfdqirk eiedetdrvt gtnkgissvp
      121 inlrvysplv lnltlvdlpg ltkvpvgdqp adieqqirdm ilqfirkenc lilavtsanq
      181 dlatsdalkl akevdpeglr tigvitkldl mdegtdardi lenkllplrr gyvgvvnrsq
      241 kdiegkkdik aameaerkff lshpayrhma drmgtpylqr vlnqqltnhi rdtlpglrdk
      301 lqkqlismek eveeyknfrp ddpsrktkam lqmiqqlqtd ferniegsgs aaintselsg
      361 garinrlfhe rfpfeivkme fdekelrkei afairnthgi rvglftpdma feaivkkqia
      421 klkepsikcv dlvvaelgnv irrcaekmsr yprlreeter iitshvrere qtakqqisll
      481 vevelaymnt nhedfigftn aqqtadvssa gkrklgnqsv favirkgwmc ihnlgimkgg
      541 srdywfvlts esiswykdee ekdkkymlpl dglkikdiea gfmsrrntfa lfnpdqrrnv
      601 ykdykqlels cesqeevdsw kasflragvy peraqsaagd geggtdgggp egsgssmdpq
      661 lerqvetirn lvdsymriit ktfrdlvpki imhltindtk efiysellaa lyasgdqtql
      721 meespeeaqk rdemlrmyha ckealriigd vsmqtsyqpt pppvkddwlk mqppdsprpa
      781 ppspggprrn vgssrapppv paaasrpapv ipsrpestgr appaapprpi sagfqaplvp
      841 srgslpsvpp rvaqgavdtm ldafgmpmsk
//
LOCUS       XP_077505056             869 aa            linear   INV 25-NOV-2025
DEFINITION  dynamin-1 shibire isoform X7 [Amblyomma americanum].
ACCESSION   XP_077505056
VERSION     XP_077505056.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648930.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..869
                     /product="dynamin-1 shibire isoform X7"
                     /calculated_mol_wt=97389
     Region          7..246
                     /region_name="DYNc"
                     /note="Dynamin, GTPase; smart00053"
                     /db_xref="CDD:197491"
     Site            39..46
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206738"
     Site            order(40,42..47,60..61,66,137,207,209..210,236..240,243)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206738"
     Site            order(41..42,141,154,179,181,183..186,188..189,210,
                     212..215,239..241)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:206738"
     Site            66
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206738"
     Site            70..72
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206738"
     Site            137..140
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206738"
     Site            order(139..140,170..171)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206738"
     Site            206..209
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206738"
     Region          216..500
                     /region_name="Dynamin_M"
                     /note="Dynamin central region; pfam01031"
                     /db_xref="CDD:460033"
     Site            236..238
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206738"
     Region          529..638
                     /region_name="PH_dynamin"
                     /note="Dynamin pleckstrin homology (PH) domain; cd01256"
                     /db_xref="CDD:269958"
     Site            order(529,531..532,534,551,620..621,624,627..628,631)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:269958"
     Region          666..756
                     /region_name="GED"
                     /note="Dynamin GTPase effector domain; pfam02212"
                     /db_xref="CDD:460495"
     CDS             1..869
                     /gene="shi"
                     /coded_by="XM_077648930.1:162..2771"
                     /db_xref="GeneID:144114909"
ORIGIN      
        1 msgndgmegl ipivnrlqda yaqlgssltl dlpqiavvgg qsagkssvle nfvgrdflpr
       61 gsgivtrrpl vlqlincnte ygeflhcrgk kftdfdqirk eiedetdrvt gtnkgissvp
      121 inlrvysplv lnltlvdlpg ltkvpvgdqp adieqqirdm ilqfirkenc lilavtsanq
      181 dlatsdalkl akevdpeglr tigvitkldl mdegtdardi lenkllplrr gyvgvvnrsq
      241 kdiegkkdik aameaerkff lshpayrhma drmgtpylqr vlnqqltnhi rdtlpglrdk
      301 lqkqlismek eveeyknfrp ddpsrktkam lqmiqqlqtd ferniegsgs aaintselsg
      361 garinrlfhe rfpfeivkme fdekelrkei afairnthgi rvglftpdma feaivkkqia
      421 klkepsikcv dlvvaelgnv irrcaekmsr yprlreeter iitshvrere qtakqqisll
      481 vevelaymnt nhedfigftk ddyhsgaqqt advssagkrk lgnqsvfavi rkgwmcihnl
      541 gimkggsrdy wfvltsesis wykdeeekdk kymlpldglk ikdieagfms rrntfalfnp
      601 dqrnvykdyk qlelscesqe evdswkasfl ragvyperaq saagdgeggt dgggpegsgs
      661 smdpqlerqv etirnlvdsy mriitktfrd lvpkiimhlt indtkefiys ellaalyasg
      721 dqtqlmeesp eeaqkrdeml rmyhackeal riigdvsmqt syqptpppvk ddwlkmqppd
      781 sprpappspg gprrnvgssr apppvpaaas rpapvipsrp estgrappaa pprpisagfq
      841 aplvpsrpvp svppripdrp qvsssqwpy
//
LOCUS       XP_077505057             866 aa            linear   INV 25-NOV-2025
DEFINITION  dynamin-1 shibire isoform X8 [Amblyomma americanum].
ACCESSION   XP_077505057
VERSION     XP_077505057.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648931.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..866
                     /product="dynamin-1 shibire isoform X8"
                     /calculated_mol_wt=96822
     Region          7..246
                     /region_name="DYNc"
                     /note="Dynamin, GTPase; smart00053"
                     /db_xref="CDD:197491"
     Site            39..46
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206738"
     Site            order(40,42..47,60..61,66,137,207,209..210,236..240,243)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206738"
     Site            order(41..42,141,154,179,181,183..186,188..189,210,
                     212..215,239..241)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:206738"
     Site            66
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206738"
     Site            70..72
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206738"
     Site            137..140
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206738"
     Site            order(139..140,170..171)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206738"
     Site            206..209
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206738"
     Region          216..503
                     /region_name="Dynamin_M"
                     /note="Dynamin central region; pfam01031"
                     /db_xref="CDD:460033"
     Site            236..238
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206738"
     Region          517..629
                     /region_name="PH_dynamin"
                     /note="Dynamin pleckstrin homology (PH) domain; cd01256"
                     /db_xref="CDD:269958"
     Site            order(519,521..522,524,541,611..612,615,618..619,622)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:269958"
     Region          657..747
                     /region_name="GED"
                     /note="Dynamin GTPase effector domain; pfam02212"
                     /db_xref="CDD:460495"
     CDS             1..866
                     /gene="shi"
                     /coded_by="XM_077648931.1:164..2764"
                     /db_xref="GeneID:144114909"
ORIGIN      
        1 msgndgmegl ipivnrlqda yaqlgssltl dlpqiavvgg qsagkssvle nfvgrdflpr
       61 gsgivtrrpl vlqlincnte ygeflhcrgk kftdfdqirk eiedetdrvt gtnkgissvp
      121 inlrvysplv lnltlvdlpg ltkvpvgdqp adieqqirdm ilqfirkenc lilavtsanq
      181 dlatsdalkl akevdpeglr tigvitkldl mdegtdardi lenkllplrr gyvgvvnrsq
      241 kdiegkkdik aameaerkff lshpayrhma drmgtpylqr vlnqqltnhi rdtlpglrdk
      301 lqkqlismek eveeyknfrp ddpsrktkam lqmiqqlqtd ferniegsgs aaintselsg
      361 garinrlfhe rfpfeivkme fdekelrkei afairnthgi rvglftpdma feaivkkqia
      421 klkepsikcv dlvvaelgnv irrcaekmsr yprlreeter iitshvrere qtakqqisll
      481 vevelaymnt nhedfigftn aqqtadvssa gkrklgnqvi rkgwmcihnl gimkggsrdy
      541 wfvltsesis wykdeeekdk kymlpldglk ikdieagfms rrntfalfnp dqrrnvykdy
      601 kqlelscesq eevdswkasf lragvypera qsaagdgegg tdgggpegsg ssmdpqlerq
      661 vetirnlvds ymriitktfr dlvpkiimhl tindtkefiy sellaalyas gdqtqlmees
      721 peeaqkrdem lrmyhackea lriigdvsmq tsyqptpppv kddwlkmqpp dsprpappsp
      781 ggprrnvgss rapppvpaaa srpapvipsr pestgrappa apprpisagf qaplvpsrgs
      841 lpsvpprvaq gavdtmldaf gmpmsk
//
LOCUS       XP_077505058             865 aa            linear   INV 25-NOV-2025
DEFINITION  dynamin-1 shibire isoform X9 [Amblyomma americanum].
ACCESSION   XP_077505058
VERSION     XP_077505058.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648932.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..865
                     /product="dynamin-1 shibire isoform X9"
                     /calculated_mol_wt=96666
     Region          7..246
                     /region_name="DYNc"
                     /note="Dynamin, GTPase; smart00053"
                     /db_xref="CDD:197491"
     Site            39..46
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206738"
     Site            order(40,42..47,60..61,66,137,207,209..210,236..240,243)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206738"
     Site            order(41..42,141,154,179,181,183..186,188..189,210,
                     212..215,239..241)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:206738"
     Site            66
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206738"
     Site            70..72
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206738"
     Site            137..140
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206738"
     Site            order(139..140,170..171)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206738"
     Site            206..209
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206738"
     Region          216..503
                     /region_name="Dynamin_M"
                     /note="Dynamin central region; pfam01031"
                     /db_xref="CDD:460033"
     Site            236..238
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206738"
     Region          517..628
                     /region_name="PH_dynamin"
                     /note="Dynamin pleckstrin homology (PH) domain; cd01256"
                     /db_xref="CDD:269958"
     Site            order(519,521..522,524,541,610..611,614,617..618,621)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:269958"
     Region          656..746
                     /region_name="GED"
                     /note="Dynamin GTPase effector domain; pfam02212"
                     /db_xref="CDD:460495"
     CDS             1..865
                     /gene="shi"
                     /coded_by="XM_077648932.1:163..2760"
                     /db_xref="GeneID:144114909"
ORIGIN      
        1 msgndgmegl ipivnrlqda yaqlgssltl dlpqiavvgg qsagkssvle nfvgrdflpr
       61 gsgivtrrpl vlqlincnte ygeflhcrgk kftdfdqirk eiedetdrvt gtnkgissvp
      121 inlrvysplv lnltlvdlpg ltkvpvgdqp adieqqirdm ilqfirkenc lilavtsanq
      181 dlatsdalkl akevdpeglr tigvitkldl mdegtdardi lenkllplrr gyvgvvnrsq
      241 kdiegkkdik aameaerkff lshpayrhma drmgtpylqr vlnqqltnhi rdtlpglrdk
      301 lqkqlismek eveeyknfrp ddpsrktkam lqmiqqlqtd ferniegsgs aaintselsg
      361 garinrlfhe rfpfeivkme fdekelrkei afairnthgi rvglftpdma feaivkkqia
      421 klkepsikcv dlvvaelgnv irrcaekmsr yprlreeter iitshvrere qtakqqisll
      481 vevelaymnt nhedfigftn aqqtadvssa gkrklgnqvi rkgwmcihnl gimkggsrdy
      541 wfvltsesis wykdeeekdk kymlpldglk ikdieagfms rrntfalfnp dqrnvykdyk
      601 qlelscesqe evdswkasfl ragvyperaq saagdgeggt dgggpegsgs smdpqlerqv
      661 etirnlvdsy mriitktfrd lvpkiimhlt indtkefiys ellaalyasg dqtqlmeesp
      721 eeaqkrdeml rmyhackeal riigdvsmqt syqptpppvk ddwlkmqppd sprpappspg
      781 gprrnvgssr apppvpaaas rpapvipsrp estgrappaa pprpisagfq aplvpsrgsl
      841 psvpprvaqg avdtmldafg mpmsk
//
LOCUS       XP_077505059             863 aa            linear   INV 25-NOV-2025
DEFINITION  dynamin-1 shibire isoform X10 [Amblyomma americanum].
ACCESSION   XP_077505059
VERSION     XP_077505059.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648933.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..863
                     /product="dynamin-1 shibire isoform X10"
                     /calculated_mol_wt=96700
     Region          7..246
                     /region_name="DYNc"
                     /note="Dynamin, GTPase; smart00053"
                     /db_xref="CDD:197491"
     Site            39..46
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206738"
     Site            order(40,42..47,60..61,66,137,207,209..210,236..240,243)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206738"
     Site            order(41..42,141,154,179,181,183..186,188..189,210,
                     212..215,239..241)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:206738"
     Site            66
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206738"
     Site            70..72
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206738"
     Site            137..140
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206738"
     Site            order(139..140,170..171)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206738"
     Site            206..209
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206738"
     Region          216..503
                     /region_name="Dynamin_M"
                     /note="Dynamin central region; pfam01031"
                     /db_xref="CDD:460033"
     Site            236..238
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206738"
     Region          523..632
                     /region_name="PH_dynamin"
                     /note="Dynamin pleckstrin homology (PH) domain; cd01256"
                     /db_xref="CDD:269958"
     Site            order(523,525..526,528,545,614..615,618,621..622,625)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:269958"
     Region          660..750
                     /region_name="GED"
                     /note="Dynamin GTPase effector domain; pfam02212"
                     /db_xref="CDD:460495"
     CDS             1..863
                     /gene="shi"
                     /coded_by="XM_077648933.1:153..2744"
                     /db_xref="GeneID:144114909"
ORIGIN      
        1 msgndgmegl ipivnrlqda yaqlgssltl dlpqiavvgg qsagkssvle nfvgrdflpr
       61 gsgivtrrpl vlqlincnte ygeflhcrgk kftdfdqirk eiedetdrvt gtnkgissvp
      121 inlrvysplv lnltlvdlpg ltkvpvgdqp adieqqirdm ilqfirkenc lilavtsanq
      181 dlatsdalkl akevdpeglr tigvitkldl mdegtdardi lenkllplrr gyvgvvnrsq
      241 kdiegkkdik aameaerkff lshpayrhma drmgtpylqr vlnqqltnhi rdtlpglrdk
      301 lqkqlismek eveeyknfrp ddpsrktkam lqmiqqlqtd ferniegsgs aaintselsg
      361 garinrlfhe rfpfeivkme fdekelrkei afairnthgi rvglftpdma feaivkkqia
      421 klkepsikcv dlvvaelgnv irrcaekmsr yprlreeter iitshvrere qtakqqisll
      481 vevelaymnt nhedfigftn aqqtadvssa gkrklgnqsv favirkgwmc ihnlgimkgg
      541 srdywfvlts esiswykdee ekdkkymlpl dglkikdiea gfmsrrntfa lfnpdqrnvy
      601 kdykqlelsc esqeevdswk asflragvyp eraqsaagdg eggtdgggpe gsgssmdpql
      661 erqvetirnl vdsymriitk tfrdlvpkii mhltindtke fiysellaal yasgdqtqlm
      721 eespeeaqkr demlrmyhac kealriigdv smqtsyqptp ppvkddwlkm qppdsprpap
      781 pspggprrnv gssrapppvp aaasrpapvi psrpestgra ppaapprpis agfqaplvps
      841 rpvpsvppri pdrpqvsssq wpy
//
LOCUS       XP_077505060             377 aa            linear   INV 25-NOV-2025
DEFINITION  F-box/LRR-repeat protein 12-like [Amblyomma americanum].
ACCESSION   XP_077505060
VERSION     XP_077505060.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648934.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..377
                     /product="F-box/LRR-repeat protein 12-like"
                     /calculated_mol_wt=42356
     Region          34..78
                     /region_name="F-box-like"
                     /note="pfam12937"
                     /db_xref="CDD:463757"
     Region          <129..>312
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          134..161
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          162..189
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          190..214
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          215..241
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          242..266
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          267..293
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          294..318
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          319..343
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     CDS             1..377
                     /gene="LOC144114911"
                     /coded_by="XM_077648934.1:153..1286"
                     /db_xref="GeneID:144114911"
ORIGIN      
        1 mgnvfvgsgd slkeialthf stlrfskrrf tttidhlpdy vllnifnyld flelcviglv
       61 crkwyalsrd rtlhrrvdls hvqltpkqlk lllqmrampp aveelrvfgd hhclwacsvs
      121 pralrriqsr cprlrrlrih nfsfrsssrd kcvrladfpd tlehlslrgs ivgrssffka
      181 srqgqslsrl qvldlgrcff vadaeakpwp wlpsltqlyl egcpfldstg yfqdmlrkls
      241 kltlldvegt yiaenaletv ashcsllehl yvgctatkds slasmgnfgg maklqsiclv
      301 qtqvtdvgll hlarvapelk vvmlemdqgi sdqgiaefcs rlpgvavrig eciglvsavf
      361 rhegcghyel ryvdsta
//
LOCUS       XP_077505061            1797 aa            linear   INV 25-NOV-2025
DEFINITION  collagen type IV alpha 1 [Amblyomma americanum].
ACCESSION   XP_077505061
VERSION     XP_077505061.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648935.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1797
                     /product="collagen type IV alpha 1"
                     /calculated_mol_wt=174657
     Region          <80..>344
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <259..>546
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <454..>648
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <590..>839
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <803..>1068
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <1003..>1253
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <1169..>1417
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          1409..1465
                     /region_name="Collagen"
                     /note="Collagen triple helix repeat (20 copies);
                     pfam01391"
                     /db_xref="CDD:460189"
     Region          <1485..>1587
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          1570..1675
                     /region_name="C4"
                     /note="C-terminal tandem repeated domain in type 4
                     procollagen; pfam01413"
                     /db_xref="CDD:460201"
     Region          1678..1791
                     /region_name="C4"
                     /note="C-terminal tandem repeated domain in type 4
                     procollagen; pfam01413"
                     /db_xref="CDD:460201"
     CDS             1..1797
                     /gene="Col4a1"
                     /coded_by="XM_077648935.1:323..5716"
                     /db_xref="GeneID:144114912"
ORIGIN      
        1 mealglarrl lllasfalcf ysaassnypa eyreyyrdps rynqsrwgng wdqsggrrgy
       61 nnyaeqqrnc sscsgcrcvg ekgsrgaagq pgppgptgiq ghpgpeglpg dkgekgeagr
      121 dgprgpkger gkmgmpgfpg ingipgipgs pgsrgvpgld gcngtkgdpg pvgplgpfgp
      181 pgdpgppgpp gprgepaygp pgskgekgep grdgqvgppg ppgadgrpgp kgdigaegpp
      241 gfrgndgpig pkgnmgigft gskgepgekg gqgprgppgn csephvgppe svvgppgerg
      301 pkggkgepgm kgepgqpgnp gpsgkhgdig mkgekglpgh pgprgkdgyp gpvgppgfkg
      361 drgdeglagl kgvsglrgdp gvpgppgprg lpgppglpgg slgragpagp pgpkgnagpp
      421 gppgkdgfpg dlgprglpgl pggpgqpgip gregppgakg dkgdnglpgl pglegppgfp
      481 gasgpkgepg pkgvpgisyp gikgmdgsng ldgfpgakge mgpkgergap gdsrdgipgr
      541 pgpigpkgek gfdgfpgtpg tpgtpgakgd kggsclscvp gtkgekgerg rdgspgqagp
      601 rglegpqgap gergddglpg lpgppgtegi pgrpgrdger gekgepalfd iavvppgvgq
      661 kgdkglqgpp gppgfdgrdg ppglpgpdgl rglkgekgls gvpgipgkpg pdgrpgpagq
      721 kgdtgygrkg eqgdrgpkgv rgydgakgek girgppgpgs lldpvrgppg ppgekgrpgl
      781 dglqgpkgda gipgrdgysg vkgekgdsgy pgqpgprglp gprgekgemg ppgfpgeagt
      841 kgnpgppgle gppgpigepg fpgagqpgpp glpgfqglkg drgypgapgl pgelglpglp
      901 gakgdtglpg lpglmgekgs kgeaaregtp gppgspglpg lpgekgepgy pglsgspgtp
      961 glqglkgesg fpgmpglpgf dgppgppgep grdgtvgfpg lpgekgdkgg agfpgipgqr
     1021 gtdglpgalg ekgdagypga pgfdgppgap gakgepglpg lqgqkgvpgd vsekgdrgyp
     1081 glpgppglvg ppgspgydgv mgpkgepgap gqglpglqga pgpkgadgfp gvpgtpgekg
     1141 aegapgfpgv kgdrgfpgip gppgqpgrdg apgipgpkgl dgapglpgik gdrgqdgfpg
     1201 vpgpvgppgp tgspgfpgqd glpgfkgdrg ypglpgekge kgvkgdegap gfpgipgekg
     1261 nrgydgtpgl agdkgdrgip gvpglsgldg erglpgppgp qglpglpgig gigpqgekgd
     1321 vgrpgldgfp glpglkgdag fpglpgqrgp pgesplegip gqkgergypg ppgppgqngy
     1381 pgpkgqdgfp gldgekgepg ypgppgiaga pgqpglpgqd gfpglpgmkg dkgdagfpgl
     1441 pglvglkgep glpgfagldg fpglpglsge kgepgpanlv kgekgerglp gpsglpgpkg
     1501 spgapgfpgl kgdagrpgpq gppgpvgpvg pkgdrglqgp aglpglpgsq gqpgnpgapg
     1561 lpggdylmgi llvrhsqspe vprcfngmtk lwdgysllyi egnekshnqd lgyagsclrr
     1621 fstmpflfcd fnsvcnyasr ndksywlstn apipmmpvge dtirqyisrc vvceapanvi
     1681 avhsqsmsip ecpsggwnql wigysfamht aagaegggqs lsspgscled fratpfiecn
     1741 gargtchyfa nklsfwlsti eqdfqferpv setlkagklr nrvsrcavci kdsnigf
//
LOCUS       XP_077505062            1734 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114913 [Amblyomma americanum].
ACCESSION   XP_077505062
VERSION     XP_077505062.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648936.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1734
                     /product="uncharacterized protein LOC144114913"
                     /calculated_mol_wt=170552
     Region          <30..>314
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <228..>461
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <358..>590
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <484..>740
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <739..>988
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <928..1190
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <1179..>1452
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          1508..1614
                     /region_name="C4"
                     /note="C-terminal tandem repeated domain in type 4
                     procollagen; pfam01413"
                     /db_xref="CDD:460201"
     Region          1618..1729
                     /region_name="C4"
                     /note="C-terminal tandem repeated domain in type 4
                     procollagen; pfam01413"
                     /db_xref="CDD:460201"
     CDS             1..1734
                     /gene="LOC144114913"
                     /coded_by="XM_077648936.1:817..6021"
                     /db_xref="GeneID:144114913"
ORIGIN      
        1 mqrmlhspcl ckgekgaggh pglpgspgte gspgplgplg ppgrrgergl tgdtgatgpk
       61 girgdegatg fpgrpgapgk pgldgwpgep gipgcngskg algpegppgv rgrvgprgpr
      121 gdygpkglpg dgginslgvr gepgedgrpg spgapgfpgf hgvkgvrgdt gpqgrpgppg
      181 gpgpkgvqgd gvqgdkgskg epgplgdigp hgdsyylegk ppfehvpger gpmgekgdvg
      241 svgergsrgv dgeiglpgtd glkgqkgdqg eegprgkrgk vgvegpagek gqkgepgftg
      301 qdgfpgdtge sgepgrlgsq gqqgppgppg ptietgeaip gprgpqgvpg dtgfpgrdge
      361 kgavgpmgyr gtqggpgvpg eqgatgprgi aikgepgedg hpglpglrga tgaqgpqgpp
      421 geqgqpgltv hgppgedgrp grdgrpgipg vrgdpgppgd kgtpglgvar qgpdgppglr
      481 gpqgdpglpg qpgrpgedgr tgprgddcgy cppgipgarg epgddgrqgp dgsmgfpgnv
      541 gpqgvkgdrg ypgargipgl pgrdgeqgid gqkgekgfpg dyifppgplp pgsikgerge
      601 rgdygprgls gregnrgpdg lpgiigvkgd mgevgdpgyp gadgaegfpg lagrkgeagd
      661 yyigekgdyg eegepgepga tgppgdmgdp glpvnvaenq thligergap gapglpgreg
      721 pkgdkgtagy dglpgrqgdk gdhgtpgysl qgpgglkgyp glggdqglpg rrgtpgpsge
      781 rgydgrpgpk ggrgrpgtgk gtgppglpgi lgpkgeqglp gypgapgpag qpgfagtkgq
      841 kgepgyngyd gpkgvkgdpg dagigtqgnp glpgydgqkg ergipglpgi dgfpglpgak
      901 gepgaagapg prgrdgqpgg rgqpgtpgle gypgtpgrkg eagdfgppgw dglagepgyp
      961 glkgtkgerg dsgqpgfpgl pgrpglager gdlgfpglpg qkgdraqrgi rgedgfpgie
     1021 gapgpkgsag fpglpgrkge rgepgralga pdgppgepgp rgydgfpglp gikgepgnpg
     1081 apgplglegd pgfpgvagld ganglpglpg lpgpdgfpgl pgfpglkgdp gfdgrpgpag
     1141 pagppglpgs pgldgkpglk gptgdpgyrg lstfeiskgd kgepgvpglp gsrgppgddg
     1201 fpgvrglkgq igdpgydgtp gtvgrpgpkg ypgnpgppgp egapafdiyp gpkgeegapg
     1261 ppgfpgipgl kgeagepgtp gpdgppgypg vppigfkgeq gdsgligapg spgqdgvpgv
     1321 pgyagipgak girgdpglag ptipgppgip gsrgqdgqrg nrgekgapgd sglpgfpgtk
     1381 gergedglpg ppgrpgingt kgskgergss espsslrlql qgfqgdpgyp gtdgspglpg
     1441 dqgfpginge pgdyglpgfp gppgyagprg lsgddgfqgl pgypgqpgtp grpgnrgppg
     1501 gpaksrgyff trhsqtsrip ecprgthtlw sgysllyilg dekahgqdlg sagsclrrfs
     1561 tmpflfcsln nvcnfasrnd ysywlstpep mpmamtpivg rdiqkyisrc svcesstqvi
     1621 amhsqtlevp ecpegwdgiw sgysffmntd sgaegsgqpl vspgscledf rpnpfiechg
     1681 hgrcnyytta ysywlatier qdmfrppdpq tlkagdlrtr vsrckvcrrq vfvp
//
LOCUS       XP_077505063             370 aa            linear   INV 25-NOV-2025
DEFINITION  chitotriosidase-1-like [Amblyomma americanum].
ACCESSION   XP_077505063
VERSION     XP_077505063.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648937.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..370
                     /product="chitotriosidase-1-like"
                     /calculated_mol_wt=41021
     Region          13..363
                     /region_name="GH18_chitinase-like"
                     /note="The GH18 (glycosyl hydrolase, family 18) type II
                     chitinases hydrolyze chitin, an abundant polymer of
                     beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a
                     major component of the cell wall of fungi and the
                     exoskeleton of arthropods. Chitinases have...; cl10447"
                     /db_xref="CDD:471972"
     Site            order(16,41,118,120,122,191..192,248,336)
                     /site_type="active"
                     /db_xref="CDD:119349"
     CDS             1..370
                     /gene="LOC144114914"
                     /coded_by="XM_077648937.1:226..1338"
                     /db_xref="GeneID:144114914"
ORIGIN      
        1 mtptsnqnqs vlivsyyswd ksslkpddid tslcthlilg fadvedrils kgstdgepsy
       61 ravaglkakn phlkvmlsvg ggahsqsfhd mvadpsntdr fvasvvetlr gnnldgidvd
      121 wefpmdirng kahftrllqk lrtafddeak asgrqslils aavaaqsvlv kasydvpeie
      181 ryvdfinlmt ydlhlfkwyy pfsghnsply grrreigyfa tlnmassakf wssrgmpkak
      241 iivgiptygl vwklchriwh gvscltlgkg tvgggyisyp evckvisngg krifdkqskv
      301 pyvidkknwi syddvssvel karwilsegf ggimtfslnc ddhtgatspe gvrfplhkki
      361 kevveqgfna
//
LOCUS       XP_077505064            1878 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger SWIM domain-containing dorado isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505064
VERSION     XP_077505064.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648938.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1878
                     /product="zinc finger SWIM domain-containing dorado
                     isoform X1"
                     /calculated_mol_wt=201156
     Region          110..>252
                     /region_name="SWIM"
                     /note="Uncharacterized protein, contains SWIM-type Zn
                     finger domain [Function unknown]; COG4715"
                     /db_xref="CDD:443750"
     CDS             1..1878
                     /gene="Dora"
                     /coded_by="XM_077648938.1:535..6171"
                     /db_xref="GeneID:144114915"
ORIGIN      
        1 mfdwdegdrf sfedsdrfee dslcswisep eslcnnwrgw krptgnpsha salhgghcrs
       61 kgeagqvlpl velaarevaa hipfevvehv yppvpeqlql riafwsfpen eedirlyscl
      121 angspeefqr gehlfkakat kdllqigfhl satvhppqsl pvskgsynva vtfdrcrits
      181 cnctcssvas wcshvvavcl hrihqpnqvc lrapvsesls rlhrdqlqkf aqyliselpq
      241 qilptaqrll dellssqqta intlrgapdp tagasayeqt twcldegtlh enirkilikl
      301 cvpspmvfsd vnylsstapp aatewcsllr plrgrepegm wnllsivrem frrsdrnaip
      361 llqilteevl aceqilcwwf ntkvslhtgs gghggravgh gnahssqhac sslcdeivvl
      421 wrlaalspal spqdrrsffv qmgewhvktl ervckvrags snvagtgggg glsgarrsdl
      481 dvfpgfkpsm eaclldwtdy piegityadg gacrwyspym htrllehspr sdptpealgg
      541 sehlsslcrr gravdvgssa sgpshhadrr pihvlcatgh hqqltplmsl ghdlpllagn
      601 vvgvvggggs nrsslssegf cenedvvgdf llpavapgmr lsgvrepaaa csssagtpkd
      661 sttltpsgvs dpegaaalge kssspedads dwsgtregah qvpdgncsid vrsddrvtag
      721 eeyhvyyfts qgkatsgdkn rdlrvpdedc lpvqikkied pfeilfarae alhahghtqe
      781 asklavrlah ellahppsle veglvasaqp gkttgnrrgr rfnpawhqvs llasatlaka
      841 aflcqvlgen pehhhlafri glfglemsrp pastkalevk lanqeqdlvs llkkiplgda
      901 elndirekae alrdhrlqsr gdallplmls syifdalvls knntkgapsp gtklpsdetl
      961 gfeaavaalg lkanvseaeh pllcegtrrq rgdlsitllv fykddqekla kimdrlldke
     1021 vhqmyksppl ssyyststpp vvtalntafp asegtsagss ggppakaddm asaaahptsg
     1081 aaegssdnts cagaadcigd gssvaavata aaaaavaepg spppvapgga rqehsggarv
     1141 kshrhnrsag dsgdrsegas spgweenfka weakfrctnf rttkkhsagm asidssapet
     1201 tssdnsptvv rrggwgrpcg pgsdsgssge ssdsvvssss gdratgakpr htdslcvlpa
     1261 dspppslpas vggapsrfla sgggalpgag tgtrapaava aapqkpppps rlkgkrgacp
     1321 slpnqpseas ahfmfelakt vltkaggnss tavlftqpsa sqnhrgphra lhmcafqigl
     1381 yalglhnavs pnwlsrtyss hvswitgqam eigssalmfl mdtweghltp pevasladka
     1441 srgrdpamvr aaaqlalscl phahalnpne iqrallqcke qspvvlerac lavesaakgg
     1501 gvypevlfdv arrwfelyee ttqgsgdapa sggeaaprgs sppnlaaqsl egnglpdvaa
     1561 pfvmeglgst papqlpmvpp lqytlpyrtp hypylsglpt fhhshllvtq hpaylqgppt
     1621 yhypplpsha qfyaaasrhg appqapppay thqmigvhvt pmaravlpma ppppappppp
     1681 aaaqvgqthq lnqtqlnyll ssyrvgllam etlarrvhdd rpqakyarnp pygedvkwll
     1741 gvakklgtsy lqefcictvn svaspfilyd ivtdtahhls rsssaslsmp irshvltpli
     1801 qkcqqmfiac thtkmyhits veydefintl rsaraafmlt pggmvqftel lhslrrsksc
     1861 kkdlwqrinn mvsssppi
//
LOCUS       XP_077505065            1877 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger SWIM domain-containing dorado isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505065
VERSION     XP_077505065.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648939.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1877
                     /product="zinc finger SWIM domain-containing dorado
                     isoform X2"
                     /calculated_mol_wt=201028
     Region          110..>252
                     /region_name="SWIM"
                     /note="Uncharacterized protein, contains SWIM-type Zn
                     finger domain [Function unknown]; COG4715"
                     /db_xref="CDD:443750"
     CDS             1..1877
                     /gene="Dora"
                     /coded_by="XM_077648939.1:535..6168"
                     /db_xref="GeneID:144114915"
ORIGIN      
        1 mfdwdegdrf sfedsdrfee dslcswisep eslcnnwrgw krptgnpsha salhgghcrs
       61 kgeagqvlpl velaarevaa hipfevvehv yppvpeqlql riafwsfpen eedirlyscl
      121 angspeefqr gehlfkakat kdllqigfhl satvhppqsl pvskgsynva vtfdrcrits
      181 cnctcssvas wcshvvavcl hrihqpnqvc lrapvsesls rlhrdqlqkf aqyliselpq
      241 qilptaqrll dellssqqta intlrgapdp tagasayeqt twcldegtlh enirkilikl
      301 cvpspmvfsd vnylsstapp aatewcsllr plrgrepegm wnllsivrem frrsdrnaip
      361 llqilteevl aceqilcwwf ntkvslhtgs gghggravgh gnahssqhac sslcdeivvl
      421 wrlaalspal spqdrrsffv qmgewhvktl ervckvrags snvagtgggg glsgarrsdl
      481 dvfpgfkpsm eaclldwtdy piegityadg gacrwyspym htrllehspr sdptpealgg
      541 sehlsslcrr gravdvgssa sgpshhadrr pihvlcatgh hqltplmslg hdlpllagnv
      601 vgvvggggsn rsslssegfc enedvvgdfl lpavapgmrl sgvrepaaac sssagtpkds
      661 ttltpsgvsd pegaaalgek ssspedadsd wsgtregahq vpdgncsidv rsddrvtage
      721 eyhvyyftsq gkatsgdknr dlrvpdedcl pvqikkiedp feilfaraea lhahghtqea
      781 sklavrlahe llahppslev eglvasaqpg kttgnrrgrr fnpawhqvsl lasatlakaa
      841 flcqvlgenp ehhhlafrig lfglemsrpp astkalevkl anqeqdlvsl lkkiplgdae
      901 lndirekaea lrdhrlqsrg dallplmlss yifdalvlsk nntkgapspg tklpsdetlg
      961 feaavaalgl kanvseaehp llcegtrrqr gdlsitllvf ykddqeklak imdrlldkev
     1021 hqmyksppls syyststppv vtalntafpa segtsagssg gppakaddma saaahptsga
     1081 aegssdntsc agaadcigdg ssvaavataa aaaavaepgs pppvapggar qehsggarvk
     1141 shrhnrsagd sgdrsegass pgweenfkaw eakfrctnfr ttkkhsagma sidssapett
     1201 ssdnsptvvr rggwgrpcgp gsdsgssges sdsvvssssg dratgakprh tdslcvlpad
     1261 spppslpasv ggapsrflas gggalpgagt gtrapaavaa apqkppppsr lkgkrgacps
     1321 lpnqpseasa hfmfelaktv ltkaggnsst avlftqpsas qnhrgphral hmcafqigly
     1381 alglhnavsp nwlsrtyssh vswitgqame igssalmflm dtweghltpp evasladkas
     1441 rgrdpamvra aaqlalsclp hahalnpnei qrallqckeq spvvleracl avesaakggg
     1501 vypevlfdva rrwfelyeet tqgsgdapas ggeaaprgss ppnlaaqsle gnglpdvaap
     1561 fvmeglgstp apqlpmvppl qytlpyrtph ypylsglptf hhshllvtqh paylqgppty
     1621 hypplpshaq fyaaasrhga ppqapppayt hqmigvhvtp maravlpmap pppapppppa
     1681 aaqvgqthql nqtqlnylls syrvgllame tlarrvhddr pqakyarnpp ygedvkwllg
     1741 vakklgtsyl qefcictvns vaspfilydi vtdtahhlsr sssaslsmpi rshvltpliq
     1801 kcqqmfiact htkmyhitsv eydefintlr saraafmltp ggmvqftell hslrrsksck
     1861 kdlwqrinnm vsssppi
//
LOCUS       XP_077505066            1133 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: ribosome biogenesis protein BMS1 homolog
            [Amblyomma americanum].
ACCESSION   XP_077505066
VERSION     XP_077505066.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648940.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 2 indels
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1133
                     /product="LOW QUALITY PROTEIN: ribosome biogenesis protein
                     BMS1 homolog"
                     /calculated_mol_wt=129962
     Region          38..1132
                     /region_name="BMS1"
                     /note="GTP-binding protein required for 40S ribosome
                     biogenesis [Translation, ribosomal structure and
                     biogenesis]; COG5192"
                     /db_xref="CDD:227519"
     CDS             1..1133
                     /gene="LOC144114916"
                     /coded_by="XM_077648940.1:189..3590"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 2 bases in 2 codons"
                     /db_xref="GeneID:144114916"
ORIGIN      
        1 mdddqkikah rarhsgrkae kkekknehvq eltpkqrnpk afsvqsvqka ekkfrrakdl
       61 qekrhhvplv drtplepppy ivavvgppkv gkttlvqcli knftrqfvst itgpitivsg
      121 kkrrltliec nndvncmidv akvadlvllm vdasygfeme tfeflnicqv hgfprimgvl
      181 shldviknvk tlrhtkkqmk hrfwteiyqg aklfylsgli rgeymkneih nlgrfisvmk
      241 frplqwrtsh pyllvdried vtdpellrtt pkcdrnvcly gyargasfkn nnnihipgcg
      301 dftiheigll pdpcplpdke krrslseker llyapmagvg gvvfdkdavy idlrgshshk
      361 egrgeggkqv demlqsmmkt qqtmdakiqt skfalfsngh tlsaddvent lhepesrprr
      421 palahiskgk ggssgsedgs dsdeclldeg sgaevseeeg qniilpkkrr kvsfagesgs
      481 dddddgnaad sgtenddwed assdddnddr ryrwkenmke kaaqafhars qkqrnlqfli
      541 ygneddykee eeeeiedsse flgelfrkvk kqentqaekr swdgvettrc vfteadalik
      601 deeildlitd cfvtgkwdee edaqallekd eelygdfedl eaknkedgqr eaedaaapie
      661 qngeessgrs kklsweerea qlqakreerk rklkeafdse ydhedektyf delkqelgeq
      721 aqlnqkefed lederrvqfe gyrpglylrv ellqmpcelv enfdpsypmi lggllagegr
      781 mgycqvrmkk hrwyqrilks sdplilslgw rrfqtmpvfs tqdhngrnrf lkytpkhlhc
      841 natfwgpitp qgagcvavqq vheqtanfri aatgvvldld rstqivkklk ltgnpfkiyk
      901 ktafikdmft salevtkfeg aalrtvsgir gqlkkavrap pgafratfed killsdlvfl
      961 kawtevpvqr fymtlttlla peeektkwqg mktvgqlrae rglhapvsqd siykvperkt
     1021 fhfkpfvvpk elqknlpykd kpkvksvteg gnalervavv leepekekvn lvqmmrvvqk
     1081 ekhrkmkekv iqrvrahrae crknelkqlk rqkelkkric kaltrmkkpq aka
//
LOCUS       XP_077505067             552 aa            linear   INV 25-NOV-2025
DEFINITION  locomotion-related protein Hikaru genki-like [Amblyomma
            americanum].
ACCESSION   XP_077505067
VERSION     XP_077505067.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648941.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..552
                     /product="locomotion-related protein Hikaru genki-like"
                     /calculated_mol_wt=60635
     Region          134..198
                     /region_name="CCP"
                     /note="Complement control protein (CCP) modules (aka short
                     consensus repeats SCRs or SUSHI repeats) have been
                     identified in several proteins of the complement system;
                     cd00033"
                     /db_xref="CDD:153056"
     Site            order(144,171)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     Region          <172..>198
                     /region_name="PHA02831"
                     /note="EEV host range protein; Provisional"
                     /db_xref="CDD:165176"
     Region          <331..486
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     Region          490..546
                     /region_name="CCP"
                     /note="Complement control protein (CCP) modules (aka short
                     consensus repeats SCRs or SUSHI repeats) have been
                     identified in several proteins of the complement system;
                     cd00033"
                     /db_xref="CDD:153056"
     Site            order(500,519)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     CDS             1..552
                     /gene="LOC144114917"
                     /coded_by="XM_077648941.1:57..1715"
                     /db_xref="GeneID:144114917"
ORIGIN      
        1 mvralhapsl rflvlfgvac fhpaqvtpka vggssgpatt tsgppppqps qpvasrtscp
       61 ppeivfngsl fqtdgtaqqf gnvyevrfmg lfggvakkra ckikcvngvw lgplcavesq
      121 agdggrfhsv lkpcplrpqk phlvvhhdgr plavtgltqm ahesmlsfrc aqlgrykfvg
      181 natvrclngq wsaampscle tsqqsnlsvq vpptilysve kgdhgvseeg elvvypetnv
      241 ylyclyqrfs gnpqwawtsq rdyakgwils adekqwryel liykakehds gtytcttprg
      301 qtneihvrvr dvtcppiegy gdenrvtsdh gnrmhskakf acmegynlvg seevvcspsg
      361 rwsdkaphcv viqcptipsq gnlrmstqnr tygirvhfsc psnyrlvgpp fvtcwknesw
      421 sdtpptcepi rcrsplppah grvlyggrqy pgdsvhytcn agyvligesv svcqangtws
      481 nnlpkckpac efpgqpahar vvpskfhydi gelvtvacna gfrllgsaql rcsrdghwsh
      541 plphcrrlvl hk
//
LOCUS       XP_077505068             541 aa            linear   INV 25-NOV-2025
DEFINITION  glucose transporter 1 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505068
VERSION     XP_077505068.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648942.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..541
                     /product="glucose transporter 1 isoform X1"
                     /calculated_mol_wt=59851
     Region          22..468
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(34..35,38..39,42,88,141..142,144..146,149,169,
                     172..173,176,287,290..291,294..296,299,327,331,389..390,
                     394,398,414,417..418,421..422,425)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340989"
     CDS             1..541
                     /gene="Glut1"
                     /coded_by="XM_077648942.1:258..1883"
                     /db_xref="GeneID:144114918"
ORIGIN      
        1 mcwcrcsscc rcpegltcml vlaifasvlg mfqfgynlgv inapqkvlee fisavylert
       61 dskipdnike wlwaitvavf aiggmvggis ggmmanrfgr kggllinnil gilggslmgf
      121 snasrsiell ifgrliigin cglntalvpm ylleispvpl rgglgtvsqv gvtvgmllsq
      181 vlgievilgt eegwpyllai avipavlqlv llpfcpespr ylliskqqes larealqklr
      241 cssvveddie emkteeqaqq qeakvtmmqv ittrslhlpl ligvlmhlsq qlsgiiavfy
      301 ysvrlfeaag lppstakyat igvgvvmvvm tlvsiplmdr agrrtlhlyg lggmfifsif
      361 itisllvkfl yqwitymsvv stlcfvvffa igpgtipwml maelfsqgpr paamsigvli
      421 nwsanfivsl ifpsmqaalg nytflpftll lgffwtftyk kvpetknrtf eeisalfrrd
      481 dvvsingmgt rrnslapgqd avyeekpetc ylhqhhhhhh chygkfhnrk kielnhledq
      541 l
//
LOCUS       XP_077505069             541 aa            linear   INV 25-NOV-2025
DEFINITION  glucose transporter 1 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505069
VERSION     XP_077505069.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648943.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..541
                     /product="glucose transporter 1 isoform X1"
                     /calculated_mol_wt=59851
     Region          22..468
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(34..35,38..39,42,88,141..142,144..146,149,169,
                     172..173,176,287,290..291,294..296,299,327,331,389..390,
                     394,398,414,417..418,421..422,425)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340989"
     CDS             1..541
                     /gene="Glut1"
                     /coded_by="XM_077648943.1:155..1780"
                     /db_xref="GeneID:144114918"
ORIGIN      
        1 mcwcrcsscc rcpegltcml vlaifasvlg mfqfgynlgv inapqkvlee fisavylert
       61 dskipdnike wlwaitvavf aiggmvggis ggmmanrfgr kggllinnil gilggslmgf
      121 snasrsiell ifgrliigin cglntalvpm ylleispvpl rgglgtvsqv gvtvgmllsq
      181 vlgievilgt eegwpyllai avipavlqlv llpfcpespr ylliskqqes larealqklr
      241 cssvveddie emkteeqaqq qeakvtmmqv ittrslhlpl ligvlmhlsq qlsgiiavfy
      301 ysvrlfeaag lppstakyat igvgvvmvvm tlvsiplmdr agrrtlhlyg lggmfifsif
      361 itisllvkfl yqwitymsvv stlcfvvffa igpgtipwml maelfsqgpr paamsigvli
      421 nwsanfivsl ifpsmqaalg nytflpftll lgffwtftyk kvpetknrtf eeisalfrrd
      481 dvvsingmgt rrnslapgqd avyeekpetc ylhqhhhhhh chygkfhnrk kielnhledq
      541 l
//
LOCUS       XP_077505070             541 aa            linear   INV 25-NOV-2025
DEFINITION  glucose transporter 1 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505070
VERSION     XP_077505070.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648944.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..541
                     /product="glucose transporter 1 isoform X1"
                     /calculated_mol_wt=59851
     Region          22..468
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(34..35,38..39,42,88,141..142,144..146,149,169,
                     172..173,176,287,290..291,294..296,299,327,331,389..390,
                     394,398,414,417..418,421..422,425)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340989"
     CDS             1..541
                     /gene="Glut1"
                     /coded_by="XM_077648944.1:60..1685"
                     /db_xref="GeneID:144114918"
ORIGIN      
        1 mcwcrcsscc rcpegltcml vlaifasvlg mfqfgynlgv inapqkvlee fisavylert
       61 dskipdnike wlwaitvavf aiggmvggis ggmmanrfgr kggllinnil gilggslmgf
      121 snasrsiell ifgrliigin cglntalvpm ylleispvpl rgglgtvsqv gvtvgmllsq
      181 vlgievilgt eegwpyllai avipavlqlv llpfcpespr ylliskqqes larealqklr
      241 cssvveddie emkteeqaqq qeakvtmmqv ittrslhlpl ligvlmhlsq qlsgiiavfy
      301 ysvrlfeaag lppstakyat igvgvvmvvm tlvsiplmdr agrrtlhlyg lggmfifsif
      361 itisllvkfl yqwitymsvv stlcfvvffa igpgtipwml maelfsqgpr paamsigvli
      421 nwsanfivsl ifpsmqaalg nytflpftll lgffwtftyk kvpetknrtf eeisalfrrd
      481 dvvsingmgt rrnslapgqd avyeekpetc ylhqhhhhhh chygkfhnrk kielnhledq
      541 l
//
LOCUS       XP_077505071             520 aa            linear   INV 25-NOV-2025
DEFINITION  glucose transporter 1 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505071
VERSION     XP_077505071.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648945.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..520
                     /product="glucose transporter 1 isoform X2"
                     /calculated_mol_wt=56965
     Region          22..468
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(34..35,38..39,42,88,141..142,144..146,149,169,
                     172..173,176,287,290..291,294..296,299,327,331,389..390,
                     394,398,414,417..418,421..422,425)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340989"
     CDS             1..520
                     /gene="Glut1"
                     /coded_by="XM_077648945.1:258..1820"
                     /db_xref="GeneID:144114918"
ORIGIN      
        1 mcwcrcsscc rcpegltcml vlaifasvlg mfqfgynlgv inapqkvlee fisavylert
       61 dskipdnike wlwaitvavf aiggmvggis ggmmanrfgr kggllinnil gilggslmgf
      121 snasrsiell ifgrliigin cglntalvpm ylleispvpl rgglgtvsqv gvtvgmllsq
      181 vlgievilgt eegwpyllai avipavlqlv llpfcpespr ylliskqqes larealqklr
      241 cssvveddie emkteeqaqq qeakvtmmqv ittrslhlpl ligvlmhlsq qlsgiiavfy
      301 ysvrlfeaag lppstakyat igvgvvmvvm tlvsiplmdr agrrtlhlyg lggmfifsif
      361 itisllvkfl yqwitymsvv stlcfvvffa igpgtipwml maelfsqgpr paamsigvli
      421 nwsanfivsl ifpsmqaalg nytflpftll lgffwtftyk kvpetknrtf eeisalfrrd
      481 dvvsingmgt rrnslapgqd avyeekpets icihpmavsf
//
LOCUS       XP_077505072             515 aa            linear   INV 25-NOV-2025
DEFINITION  glucose transporter 1 isoform X3 [Amblyomma americanum].
ACCESSION   XP_077505072
VERSION     XP_077505072.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648946.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..515
                     /product="glucose transporter 1 isoform X3"
                     /calculated_mol_wt=56405
     Region          22..468
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(34..35,38..39,42,88,141..142,144..146,149,169,
                     172..173,176,287,290..291,294..296,299,327,331,389..390,
                     394,398,414,417..418,421..422,425)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340989"
     CDS             1..515
                     /gene="Glut1"
                     /coded_by="XM_077648946.1:258..1805"
                     /db_xref="GeneID:144114918"
ORIGIN      
        1 mcwcrcsscc rcpegltcml vlaifasvlg mfqfgynlgv inapqkvlee fisavylert
       61 dskipdnike wlwaitvavf aiggmvggis ggmmanrfgr kggllinnil gilggslmgf
      121 snasrsiell ifgrliigin cglntalvpm ylleispvpl rgglgtvsqv gvtvgmllsq
      181 vlgievilgt eegwpyllai avipavlqlv llpfcpespr ylliskqqes larealqklr
      241 cssvveddie emkteeqaqq qeakvtmmqv ittrslhlpl ligvlmhlsq qlsgiiavfy
      301 ysvrlfeaag lppstakyat igvgvvmvvm tlvsiplmdr agrrtlhlyg lggmfifsif
      361 itisllvkfl yqwitymsvv stlcfvvffa igpgtipwml maelfsqgpr paamsigvli
      421 nwsanfivsl ifpsmqaalg nytflpftll lgffwtftyk kvpetknrtf eeisalfrrd
      481 dvvsingmgt rrnslapgqd avyeekpety gtfsa
//
LOCUS       XP_077505073            1376 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114920 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505073
VERSION     XP_077505073.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648947.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1376
                     /product="uncharacterized protein LOC144114920 isoform X1"
                     /calculated_mol_wt=141344
     Region          337..450
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          <599..989
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          882..>1099
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     Region          1112..1235
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     CDS             1..1376
                     /gene="LOC144114920"
                     /coded_by="XM_077648947.1:131..4261"
                     /db_xref="GeneID:144114920"
ORIGIN      
        1 mslqsrqasl lactsrqqel qrrlekihee kdtivntleq mvkgvdllva hvcsltnvdr
       61 lfygvvdasa ameqlylklf rihllltten nyvdaaqqra pqvaitfpgs tdanedgdsq
      121 vavidgnesq rsfqsspqrt seaspphlsl cdellgyasa psllhlkhqn lpdvlatdra
      181 vcrtvsslkt lpivyarktp alpesehlip gieacatgvs atlkqqpsae ptsitgfdhg
      241 dgcfykqend ldksaevgsc avqpnipfta phsvqsqdsq tgaagcgtsv ssppppervy
      301 ysstlvpsts gagqdssklv hheiptfspd imnstqnvvl sfyknpaefw lqldtsakel
      361 evflrklheh yscsarrqgf tarpgmscaa fyaedghwyr akivrvfpdh vtvyyvdfgn
      421 tdsvdlnslc ildqqfatlp aqalycgikg lsvpcgtgaf sleavrsfre risaqnvklq
      481 affhtrdfst ryhvdimarp ledtvmnlsq eflascvker spllctsedt enlfnqeasr
      541 skaasssaap ssaapppagm hskppgappa gapaasmtgp gmasagmapa amapagmttp
      601 vsaptitasa graeavmtvp vftaalmapp gmaapgfipa gmaapcltpa vmtapgftpa
      661 gmavpgltga glappgltga cvappgltaa gmappglnpa gmvapgltaa gmvahgltaa
      721 gmapprpagm atpgmnpagm vapgltaagm mapgltaagm aapgltaagm aapgltpagm
      781 avpcltpagm avpgltpagm avpgltpagm avpgltpagm avpgltpagl avpgwtpagm
      841 vapvsttagr avpnltpagt aapgstpvgt aapgstpvgt aapgstpvgt aelgstpvgt
      901 aepgstpvgt aepgstpvgt aepgstpvgt aepgstpvgt aepgsspfgt aepgstpfrt
      961 aepgstpfgt aepgstpfgt aepgstpfgt aepgstpfgt aepgttpagm avlntntsgm
     1021 appgltvpnn tipglavygl atpsltaala acgtpspssl gqeisaqqma lsqitqiemg
     1081 ssgikapvas fpfpvgllpp vivppailpe gdsfsvilsv vfnptdfygq vvdnvnttsk
     1141 vleqlqqhln mhgiqtqapa eesvakgsfw mclfegdknw yrvqvldiir taggrrfrll
     1201 yvdygnrttv tstclrplpy slsglpacay rmalaliapk ngrkwdpvat gifvqetgfk
     1261 amliaekkgf rhcgyttpnl akcyrrlstl gthckkrlnv daatsttvwv trggaptvfs
     1321 rssackctra kawppgtwts leniidvvlw nkngepavnl naylveqdva vlkpll
//
LOCUS       XP_077505075            1342 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114920 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505075
VERSION     XP_077505075.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648949.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1342
                     /product="uncharacterized protein LOC144114920 isoform X2"
                     /calculated_mol_wt=137359
     Region          337..450
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          <599..989
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          882..>1099
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     Region          1112..1235
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     CDS             1..1342
                     /gene="LOC144114920"
                     /coded_by="XM_077648949.1:131..4159"
                     /db_xref="GeneID:144114920"
ORIGIN      
        1 mslqsrqasl lactsrqqel qrrlekihee kdtivntleq mvkgvdllva hvcsltnvdr
       61 lfygvvdasa ameqlylklf rihllltten nyvdaaqqra pqvaitfpgs tdanedgdsq
      121 vavidgnesq rsfqsspqrt seaspphlsl cdellgyasa psllhlkhqn lpdvlatdra
      181 vcrtvsslkt lpivyarktp alpesehlip gieacatgvs atlkqqpsae ptsitgfdhg
      241 dgcfykqend ldksaevgsc avqpnipfta phsvqsqdsq tgaagcgtsv ssppppervy
      301 ysstlvpsts gagqdssklv hheiptfspd imnstqnvvl sfyknpaefw lqldtsakel
      361 evflrklheh yscsarrqgf tarpgmscaa fyaedghwyr akivrvfpdh vtvyyvdfgn
      421 tdsvdlnslc ildqqfatlp aqalycgikg lsvpcgtgaf sleavrsfre risaqnvklq
      481 affhtrdfst ryhvdimarp ledtvmnlsq eflascvker spllctsedt enlfnqeasr
      541 skaasssaap ssaapppagm hskppgappa gapaasmtgp gmasagmapa amapagmttp
      601 vsaptitasa graeavmtvp vftaalmapp gmaapgfipa gmaapcltpa vmtapgftpa
      661 gmavpgltga glappgltga cvappgltaa gmappglnpa gmvapgltaa gmvahgltaa
      721 gmapprpagm atpgmnpagm vapgltaagm mapgltaagm aapgltaagm aapgltpagm
      781 avpcltpagm avpgltpagm avpgltpagm avpgltpagm avpgltpagl avpgwtpagm
      841 vapvsttagr avpnltpagt aapgstpvgt aapgstpvgt aapgstpvgt aelgstpvgt
      901 aepgstpvgt aepgstpvgt aepgstpvgt aepgstpvgt aepgsspfgt aepgstpfrt
      961 aepgstpfgt aepgstpfgt aepgstpfgt aepgstpfgt aepgttpagm avlntntsgm
     1021 appgltvpnn tipglavygl atpsltaala acgtpspssl gqeisaqqma lsqitqiemg
     1081 ssgikapvas fpfpvgllpp vivppailpe gdsfsvilsv vfnptdfygq vvdnvnttsk
     1141 vleqlqqhln mhgiqtqapa eesvakgsfw mclfegdknw yrvqvldiir taggrrfrll
     1201 yvdygnrttv tstclrplpy slsglpacay rmalaliapk ngrkwdpvat gifvqetgfk
     1261 amliaekkgf rhcgyttpnl akcyrrlstl gthckkrlnv daatsttvwv trggaptvfs
     1321 rssassktss msccgtktgs qq
//
LOCUS       XP_077505076            1310 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114920 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505076
VERSION     XP_077505076.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648950.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1310
                     /product="uncharacterized protein LOC144114920 isoform X3"
                     /calculated_mol_wt=134142
     Region          337..450
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          <599..989
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          882..>1099
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     Region          1112..1235
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     CDS             1..1310
                     /gene="LOC144114920"
                     /coded_by="XM_077648950.1:131..4063"
                     /db_xref="GeneID:144114920"
ORIGIN      
        1 mslqsrqasl lactsrqqel qrrlekihee kdtivntleq mvkgvdllva hvcsltnvdr
       61 lfygvvdasa ameqlylklf rihllltten nyvdaaqqra pqvaitfpgs tdanedgdsq
      121 vavidgnesq rsfqsspqrt seaspphlsl cdellgyasa psllhlkhqn lpdvlatdra
      181 vcrtvsslkt lpivyarktp alpesehlip gieacatgvs atlkqqpsae ptsitgfdhg
      241 dgcfykqend ldksaevgsc avqpnipfta phsvqsqdsq tgaagcgtsv ssppppervy
      301 ysstlvpsts gagqdssklv hheiptfspd imnstqnvvl sfyknpaefw lqldtsakel
      361 evflrklheh yscsarrqgf tarpgmscaa fyaedghwyr akivrvfpdh vtvyyvdfgn
      421 tdsvdlnslc ildqqfatlp aqalycgikg lsvpcgtgaf sleavrsfre risaqnvklq
      481 affhtrdfst ryhvdimarp ledtvmnlsq eflascvker spllctsedt enlfnqeasr
      541 skaasssaap ssaapppagm hskppgappa gapaasmtgp gmasagmapa amapagmttp
      601 vsaptitasa graeavmtvp vftaalmapp gmaapgfipa gmaapcltpa vmtapgftpa
      661 gmavpgltga glappgltga cvappgltaa gmappglnpa gmvapgltaa gmvahgltaa
      721 gmapprpagm atpgmnpagm vapgltaagm mapgltaagm aapgltaagm aapgltpagm
      781 avpcltpagm avpgltpagm avpgltpagm avpgltpagm avpgltpagl avpgwtpagm
      841 vapvsttagr avpnltpagt aapgstpvgt aapgstpvgt aapgstpvgt aelgstpvgt
      901 aepgstpvgt aepgstpvgt aepgstpvgt aepgstpvgt aepgsspfgt aepgstpfrt
      961 aepgstpfgt aepgstpfgt aepgstpfgt aepgstpfgt aepgttpagm avlntntsgm
     1021 appgltvpnn tipglavygl atpsltaala acgtpspssl gqeisaqqma lsqitqiemg
     1081 ssgikapvas fpfpvgllpp vivppailpe gdsfsvilsv vfnptdfygq vvdnvnttsk
     1141 vleqlqqhln mhgiqtqapa eesvakgsfw mclfegdknw yrvqvldiir taggrrfrll
     1201 yvdygnrttv tstclrplpy slsglpacay rmalaliapk ngrkwdpvat gifvqetgfk
     1261 amliaekkgf rhcgleniid vvlwnkngep avnlnaylve qdvavlkpll
//
LOCUS       XP_077505077             533 aa            linear   INV 25-NOV-2025
DEFINITION  aromatic-L-amino-acid decarboxylase-like [Amblyomma americanum].
ACCESSION   XP_077505077
VERSION     XP_077505077.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648951.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..533
                     /product="aromatic-L-amino-acid decarboxylase-like"
                     /calculated_mol_wt=60147
     Region          35..414
                     /region_name="Pyridoxal_deC"
                     /note="Pyridoxal-dependent decarboxylase conserved domain;
                     pfam00282"
                     /db_xref="CDD:395219"
     Site            order(149..150,153,243,272,275,301,304)
                     /site_type="other"
                     /note="pyridoxal 5'-phosphate binding site [chemical
                     binding]"
                     /db_xref="CDD:99743"
     Site            304
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:99743"
     CDS             1..533
                     /gene="LOC144114921"
                     /coded_by="XM_077648951.1:145..1746"
                     /db_xref="GeneID:144114921"
ORIGIN      
        1 mddeqfleag rqllefalsy wnglrnrpvi pdvapgylaa lvppeapqep dkwedifkdi
       61 ervilpgmth whsphfhayf ptgqsppsll adilsstfai vgftwiaspa ntelemvvmd
      121 wlgkaiglpe hflfstpgin gggviqstas ectltavlaa ktkltthllq qnpsltpcqv
      181 wdklvvyasc qahssveraa llasvrfhvl ntdgqlavrg etlravvhed rrmgfipmam
      241 vatlgttnsc afdnlaeiga vceaeslwlh vdaayagsaf icpefrhymk gvehvssfsm
      301 nphkwllvnv dcsamwfrkr sdieeafkvn piylrhdneg gkvpdyrhwt iplgrrfrsl
      361 klwfvfrcyg isglqahirr qinlarefea lvnenknfev vvpvtmalvc frfkgsnadn
      421 edllqtlhkr rtihltptkl rgvfvlrfav csrmtenedv hyawqeicaa lqtlekergs
      481 slkhedvtfs tpqgfvkrmp lqgeanrnkg pnracanmll ekynqikewi fhn
//
LOCUS       XP_077505078             235 aa            linear   INV 25-NOV-2025
DEFINITION  spaetzle domain-containing protein [Amblyomma americanum].
ACCESSION   XP_077505078
VERSION     XP_077505078.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648952.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..235
                     /product="spaetzle domain-containing protein"
                     /calculated_mol_wt=25928
     Region          125..216
                     /region_name="Spaetzle"
                     /note="pfam16077"
                     /db_xref="CDD:465007"
     CDS             1..235
                     /gene="spz"
                     /coded_by="XM_077648952.1:382..1089"
                     /db_xref="GeneID:144114922"
ORIGIN      
        1 marsalavlw latlaycggs armsptrphg fktmrsargp panrttgrpv vypasselrp
       61 rldsrgmpac aarslgesay cvedttyptq iirnvvqsln vdlsdkeila rqplnhedsi
      121 dqrvcaikrk tiypksarsa yqewlyvind veyaqavtie lcadegapce fvssglppgy
      181 tsacrqkfay rklltmhpte kkayaenfpf psccvcyvks pplaarslsp srrrn
//
LOCUS       XP_077505079             266 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114923 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505079
VERSION     XP_077505079.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648953.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..266
                     /product="uncharacterized protein LOC144114923 isoform X1"
                     /calculated_mol_wt=29750
     Region          161..255
                     /region_name="Spaetzle"
                     /note="cl29083"
                     /db_xref="CDD:475150"
     CDS             1..266
                     /gene="LOC144114923"
                     /coded_by="XM_077648953.1:73..873"
                     /db_xref="GeneID:144114923"
ORIGIN      
        1 mrpgiqlnlk tagtfkvipw hdrgpkepvr hrscapasad vvaivfacsr eclcvpivtp
       61 skgrtrfani sihrnqphrk plpslhklnt sclqdnsfce slpgypkelv tsvmqlrgsp
      121 frsyfaevdr deallddlap psseqrvrvp fgsdvdeaqv cdvrvevryp rfgvdkdgdm
      181 rlivndggdf kqpvrvevcr neggtcdrih dmlpaghstr catkyvnrvl valftednrv
      241 epkptkfafp aacvcyvrty yypskv
//
LOCUS       XP_077505080             244 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114923 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505080
VERSION     XP_077505080.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648954.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..244
                     /product="uncharacterized protein LOC144114923 isoform X2"
                     /calculated_mol_wt=27406
     Region          139..233
                     /region_name="Spaetzle"
                     /note="cl29083"
                     /db_xref="CDD:475150"
     CDS             1..244
                     /gene="LOC144114923"
                     /coded_by="XM_077648954.1:98..832"
                     /db_xref="GeneID:144114923"
ORIGIN      
        1 mpiarrawln wivalqalav aivfacsrec lcvpivtpsk grtrfanisi hrnqphrkpl
       61 pslhklntsc lqdnsfcesl pgypkelvts vmqlrgspfr syfaevdrde allddlapps
      121 seqrvrvpfg sdvdeaqvcd vrvevryprf gvdkdgdmrl ivndggdfkq pvrvevcrne
      181 ggtcdrihdm lpaghstrca tkyvnrvlva lftednrvep kptkfafpaa cvcyvrtyyy
      241 pskv
//
LOCUS       XP_077505081             492 aa            linear   INV 25-NOV-2025
DEFINITION  QRFP-like peptide receptor [Amblyomma americanum].
ACCESSION   XP_077505081
VERSION     XP_077505081.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648955.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..492
                     /product="QRFP-like peptide receptor"
                     /calculated_mol_wt=54853
     Region          48..351
                     /region_name="7tmA_CCKR-like"
                     /note="cholecystokinin receptors and related proteins,
                     member of the class A family of seven-transmembrane G
                     protein-coupled receptors; cd14993"
                     /db_xref="CDD:320124"
     Region          48..72
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320124"
     Region          79..105
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320124"
     Site            order(101,104..105,117..122,124..125,128,173,175..179,223,
                     226..228,230..232,234..235,292,295..296,298..299,302,
                     318..319,321..323,326,329..330)
                     /site_type="other"
                     /note="putative peptide ligand binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:320124"
     Region          117..147
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320124"
     Region          161..179
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320124"
     Region          223..248
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320124"
     Region          272..302
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320124"
     Region          319..344
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320124"
     CDS             1..492
                     /gene="LOC144114924"
                     /coded_by="XM_077648955.1:577..2055"
                     /db_xref="GeneID:144114924"
ORIGIN      
        1 mpgyepmean csvdlwegpl dvfnfsntsf ldmdeldstf twkeylkmsv ylvtivaalv
       61 gnfavivtvv tnrvmrttin yyllnlavad tlicifcmwv hfvnslstfy vlgsfmcrfe
      121 gfakmtcltc svltlsaisc drfiaimfpl qahvrvtkqr tsaviaviwv tsaaisvpli
      181 vfsrlytvsw tplesvtycg wtwpteatwd aergecifas sgrklyyllv tvalfflpva
      241 imltaysliv wrlwitqmpg ersaaninaq crakkkvitm vctvliafvi cwmplqvivl
      301 ysqfgdsklr dkempdwfte assvatyiay snsllnpiiy ggfnnnfrqg lcsvlqcyir
      361 rkpsqlpras ihsarmdrch ractksrctv ssfrnhhscp vpcgsgvksl verqrsyqgc
      421 sepgrpaswa vgrlvysaqr grasataqpr agdddgaerr gdddaglqch hcqqhrtgep
      481 pfggpgangn vp
//
LOCUS       XP_077505082             295 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114925 [Amblyomma americanum].
ACCESSION   XP_077505082
VERSION     XP_077505082.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648956.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..295
                     /product="uncharacterized protein LOC144114925"
                     /calculated_mol_wt=32952
     CDS             1..295
                     /gene="LOC144114925"
                     /coded_by="XM_077648956.1:22..909"
                     /db_xref="GeneID:144114925"
ORIGIN      
        1 meveaalilf erslkknglr yttmlsdgds raflalqead vygyvkveke dcinhvkkrm
       61 gtalrnliak qkgtenlggk gkltgsmitk lssyyawalk shkgdvdsmh kavmatyrhi
      121 tsndetsdhs lcpsgpnswc qhnvaasrge papkhrhnlp phvckalvpi yerlserkll
      181 erchrgktqn sneslhsliw alapkerhas lyivqaavae avmrfnagse rtskiilrel
      241 glntsakask rmtekderre hksarkhaaa envqgafkkr hldngkqrdy ipggf
//
LOCUS       XP_077505083             781 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114927 [Amblyomma americanum].
ACCESSION   XP_077505083
VERSION     XP_077505083.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648957.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..781
                     /product="uncharacterized protein LOC144114927"
                     /calculated_mol_wt=83976
     Region          249..470
                     /region_name="MSL5"
                     /note="Splicing factor (branch point binding protein) [RNA
                     processing and modification]; COG5176"
                     /db_xref="CDD:227503"
     Region          <474..>519
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     CDS             1..781
                     /gene="LOC144114927"
                     /coded_by="XM_077648957.1:287..2632"
                     /db_xref="GeneID:144114927"
ORIGIN      
        1 matganttpl grlnsfpgvr psvgdgvsll kpsymagalp paalatqang taavvpaatp
       61 pvalpvfpal ptsikaekdh derdkdrdrd rerdrdrdsr rdrdkersrd rgrdrgrars
      121 rdrsrdrhrg rsrdksrdrr drdrdkdrdr dkdrdrdkdr drdkdrerdr dkdrdreker
      181 epalpamtga ppvvsmlpsw asqdensnts mgsmgsmgsl eaintgdrrr krrsrwccde
      241 kekvfipgmp tvlptnldpn qerayllqlq ieelsrrlrt edlgipfnpe erspspepiy
      301 nsagkrlntr eyrvrkrled erhvlitemf tinpdykpps dykpplvrvs ekvmipqeeh
      361 pdinfvglli gprgntlksl eketgakiii rgkgsvkegk vgrkdgqplp gedeplhafv
      421 tassqenvrk avdrikeiir qgvevpegqn dlrrmqlrel allngtlren dllggprcsn
      481 cgapghkawq cpdrpnitnn vicaccggtg hiardcrerg kggggyggrg gfggeggpgg
      541 aqskideeym slmaelgegp pppnkaggdd tgsrgggggg ggpqlggaqg pmraimappp
      601 ltggnapmsm drqlpqqwgs gpkgmmppga prppsmmggp pppphmagtg mpwqpsqggm
      661 psmtgvpppp pmmgpppsgl ppwmtgppqg pggpwqspia gvpppppqlf pwgpggpfmn
      721 mglppppppp avtagstest apgtsssaaa tsttsttmvd lstlaalpgl laappppppp
      781 s
//
LOCUS       XP_077505084             574 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114928 [Amblyomma americanum].
ACCESSION   XP_077505084
VERSION     XP_077505084.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648958.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..574
                     /product="uncharacterized protein LOC144114928"
                     /calculated_mol_wt=64599
     CDS             1..574
                     /gene="LOC144114928"
                     /coded_by="XM_077648958.1:220..1944"
                     /db_xref="GeneID:144114928"
ORIGIN      
        1 mdirvrywda vservttryy tsvfmghsta edilekllsa ldclplskiv hlsmdgpnvn
       61 lkcfknlqsh lqnnyqvqcl elgncglhtl hnayragvma skwcldtlls slsglfhdap
      121 arrddfasvt gqttyplnfv shrwvenvpv leraillwpd vnkyvqcirl rqvslpkcia
      181 fqrvcdacad plmpaklqfa lgiamifkpf lteyqgdkpl vfflakdles vvrklmtrfv
      241 mcsvvaasag ilsflkidid dpknhaplek vgighaaeqv vkaakisakd vfafkmeckq
      301 flinaakkil dksplryplv ralsslvpvr mcsrpdeclt gfrkvlgali eanrlsehqr
      361 gavlaeytel lqeekdtlgs fekhskslde ffcnllkfrs scsnlwgvvk vllilshgqa
      421 tvergfsinl htsienmkem syvsqrivcd aidkvggilk veitkeprts tsaawnryqa
      481 yleeqkqqea qqlrqskrsl ieneieanrh kkkklektiv dltaaadsys ekaeaandit
      541 yivksnslrk tskekmqdlq eidraiaakl qals
//
LOCUS       XP_077505085             414 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114929 [Amblyomma americanum].
ACCESSION   XP_077505085
VERSION     XP_077505085.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648959.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..414
                     /product="uncharacterized protein LOC144114929"
                     /calculated_mol_wt=47344
     Region          198..359
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..414
                     /gene="LOC144114929"
                     /coded_by="XM_077648959.1:455..1699"
                     /db_xref="GeneID:144114929"
ORIGIN      
        1 msddprderk feialamsvv lsssvdlals rrkvreirrr llannallia lassrrtvrr
       61 qvwayprner wfedtlpglg srhfrqsfrv setsfrylvd vcrpamerqv tnmrepipve
      121 krvavalykl cscaedrava dvfgigrstv nelyrefcet vvatlehewv kmptavdmra
      181 hirefeamcg fpqavgaldg chiavsppke hasdyynykg wysvillalv dhkyhfrfvn
      241 agspgrchds yvyqnstlar mvespifsap tatinrvivp plilcdqafp ltpnlvkpfr
      301 sldtcdghrs ynyhlskcrr ivenafgrlk arfrliskrm danvknvaqi iraccvlhni
      361 cehfndsvaq qwlheveidd avhvqpahss dvvtangadv realveyyrq feqh
//
LOCUS       XP_077505086             184 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114930 [Amblyomma americanum].
ACCESSION   XP_077505086
VERSION     XP_077505086.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648960.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..184
                     /product="uncharacterized protein LOC144114930"
                     /calculated_mol_wt=20918
     Region          13..136
                     /region_name="TMEM72"
                     /note="Transmembrane protein family 72; pfam16054"
                     /db_xref="CDD:435102"
     CDS             1..184
                     /gene="LOC144114930"
                     /coded_by="XM_077648960.1:82..636"
                     /db_xref="GeneID:144114930"
ORIGIN      
        1 msyrpnqgly rtldfvtpvt rvwgifsaiv ywgigaditf hgdslgpyvl vsavfitlfe
       61 tafavdlfle vcireergyc lrlwrgirwv dlwrksvlyl alsilcfayh ssswlggyag
      121 attlvlcily liltyknhle vkealladke asydrfdale eevdetlpep dvdtvgdqdr
      181 ilev
//
LOCUS       XP_077505087             463 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114932 [Amblyomma americanum].
ACCESSION   XP_077505087
VERSION     XP_077505087.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648961.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..463
                     /product="uncharacterized protein LOC144114932"
                     /calculated_mol_wt=51184
     Region          159..445
                     /region_name="DUF4481"
                     /note="Domain of unknown function (DUF4481); pfam14800"
                     /db_xref="CDD:464322"
     CDS             1..463
                     /gene="LOC144114932"
                     /coded_by="XM_077648961.1:210..1601"
                     /db_xref="GeneID:144114932"
ORIGIN      
        1 msenaeskpe ltpegdaqaa phapsrersq qgdapksswv kfdddasaaa tiepsqtqtv
       61 epplpikmda llkkvpsaep rsdlgplapp rppaasscsv ppvavcppap arlkprasae
      121 pqvvsnhvap atthrlesva vplserlatr sssaagfnng diivnalptn rrcawitkae
      181 fkpelvpeel matgltltve eyvaamqvlv hdvrftlynv cykrlllvwv llgffillcl
      241 lfsgvrglal fgggvvwlvv nalgifvcmw lkfkllhmle rciasinslf frhkillgld
      301 drgkischkv hlifvyfdvs acirylyeml enqdrqdald aratgdttns arngaalypv
      361 drtrmdidms diiitgssst trisqrekya eklllrysqr wvkefvrkrl dlnmpvhpdg
      421 fedgpcppap prhcalarcp cqfieehlrf kpltkcslse lcf
//
LOCUS       XP_077505088             463 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114932 [Amblyomma americanum].
ACCESSION   XP_077505088
VERSION     XP_077505088.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648962.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..463
                     /product="uncharacterized protein LOC144114932"
                     /calculated_mol_wt=51184
     Region          159..445
                     /region_name="DUF4481"
                     /note="Domain of unknown function (DUF4481); pfam14800"
                     /db_xref="CDD:464322"
     CDS             1..463
                     /gene="LOC144114932"
                     /coded_by="XM_077648962.1:140..1531"
                     /db_xref="GeneID:144114932"
ORIGIN      
        1 msenaeskpe ltpegdaqaa phapsrersq qgdapksswv kfdddasaaa tiepsqtqtv
       61 epplpikmda llkkvpsaep rsdlgplapp rppaasscsv ppvavcppap arlkprasae
      121 pqvvsnhvap atthrlesva vplserlatr sssaagfnng diivnalptn rrcawitkae
      181 fkpelvpeel matgltltve eyvaamqvlv hdvrftlynv cykrlllvwv llgffillcl
      241 lfsgvrglal fgggvvwlvv nalgifvcmw lkfkllhmle rciasinslf frhkillgld
      301 drgkischkv hlifvyfdvs acirylyeml enqdrqdald aratgdttns arngaalypv
      361 drtrmdidms diiitgssst trisqrekya eklllrysqr wvkefvrkrl dlnmpvhpdg
      421 fedgpcppap prhcalarcp cqfieehlrf kpltkcslse lcf
//
LOCUS       XP_077505089             184 aa            linear   INV 25-NOV-2025
DEFINITION  calcium load-activated calcium channel isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505089
VERSION     XP_077505089.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648963.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..184
                     /product="calcium load-activated calcium channel isoform
                     X1"
                     /calculated_mol_wt=20998
     Region          5..162
                     /region_name="EMC3_TMCO1"
                     /note="Integral membrane protein EMC3/TMCO1-like;
                     pfam01956"
                     /db_xref="CDD:396507"
     CDS             1..184
                     /gene="LOC144114933"
                     /coded_by="XM_077648963.1:102..656"
                     /db_xref="GeneID:144114933"
ORIGIN      
        1 mwsdtllilv isiftallse gltwlmvyrt ekyqklkaev ekqskrlekk keahgeaaar
       61 qqkkkieree eklknnnrdl slvkmksmfa igfaftalls mfnsifdgrv vaklpfvpis
      121 wiqslshrnl pgedytdcsf iflyilctms irqntqkllg fapsrtankq sgtlfspgpq
      181 qltr
//
LOCUS       XP_077505090             158 aa            linear   INV 25-NOV-2025
DEFINITION  calcium load-activated calcium channel isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505090
VERSION     XP_077505090.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648964.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="calcium load-activated calcium channel isoform
                     X2"
                     /calculated_mol_wt=18188
     Region          5..153
                     /region_name="EMC3_TMCO1"
                     /note="Integral membrane protein EMC3/TMCO1-like;
                     pfam01956"
                     /db_xref="CDD:396507"
     CDS             1..158
                     /gene="LOC144114933"
                     /coded_by="XM_077648964.1:104..580"
                     /db_xref="GeneID:144114933"
ORIGIN      
        1 mwsdtllilv isiftallse gltwlmvyrt ekyqklkaev ekqskrlekk keahgeaaar
       61 qqkkkieree eklknnnrdl slvkmksmfa igfaftalls mfnsifdgrv vaklpfvpis
      121 wiqslshrnl pgedytdcsf iflyilctms irqsfavl
//
LOCUS       XP_077505091             828 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114934 [Amblyomma americanum].
ACCESSION   XP_077505091
VERSION     XP_077505091.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648965.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..828
                     /product="uncharacterized protein LOC144114934"
                     /calculated_mol_wt=93473
     Region          166..>308
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..828
                     /gene="LOC144114934"
                     /coded_by="XM_077648965.1:175..2661"
                     /db_xref="GeneID:144114934"
ORIGIN      
        1 mlghdylplp stlglgtiag adsavhdtdd phssrapqlt dvqttppppa deegaaqstp
       61 irviasaswd spdyaeagps glsgakesvr qpvmtpasgt pksrrrkgav vytprtkkrf
      121 eklhsrsdry rkalsrirkr hdtrrvtqqe alnklephlp qdlfelvkaq irlcsfpksk
      181 khwskqmkqf alnlffhspk ayrflremlh lphvrtlrrw ladipmtpgi ipgamdaies
      241 itkdwplqdk accllfdeis lkknlmydqs kdivigftdd gntrtsrvan ialvmavsgi
      301 srswmqpvaf aashnatsvl amrkllldli qqlqtkgllv kavicdqgsn nvslsrmlih
      361 sihepyfvvn nrkiyfmfdt phlikctrnn lrrhkltigs evvdwthiet lykktrhidr
      421 asktpvehlk srlakklkeq hiyrmpfadm rvkyatqvfs esvsvalltl iaiqelpvda
      481 kftaefterm dklfdclnss alkkqddklr yamtegsehh vflescidwi aqwhfggpld
      541 kqphtikgwq itikallllw tdlkadfdyt hlftrrlqqd plenffaivr qkhgcnetpn
      601 vyqftaavkh iwigqlfkls qgncevttha fltnlesvsa vlspsstpst shsrqqgsgs
      661 sdlqassqda pdivyenvvy hvagtlvksf lvqrttdctc egtllaadgg slhgrhqffa
      721 llkengvpeh lfqsiaatse aclnyikkld safrkeiscc aqlsnvsrnl vermpnqqav
      781 fcslgckikf lklfcrtrlh whlsflnrsm tsktkqsapa rkrrklag
//
LOCUS       XP_077505092             491 aa            linear   INV 25-NOV-2025
DEFINITION  FAD-dependent oxidoreductase domain-containing protein 1-like
            [Amblyomma americanum].
ACCESSION   XP_077505092
VERSION     XP_077505092.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648966.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..491
                     /product="FAD-dependent oxidoreductase domain-containing
                     protein 1-like"
                     /calculated_mol_wt=54223
     Region          76..478
                     /region_name="DadA"
                     /note="Glycine/D-amino acid oxidase (deaminating) [Amino
                     acid transport and metabolism]; COG0665"
                     /db_xref="CDD:440429"
     CDS             1..491
                     /gene="LOC144114935"
                     /coded_by="XM_077648966.1:359..1834"
                     /db_xref="GeneID:144114935"
ORIGIN      
        1 mmtplrllqr anaafsrlcq ckpsvrgcss ddgrkkvsss vgeisperrv fnifrdefrg
       61 yfkpkgsevp eplfpkevdi lvvgggimgs svaywlkqra pesfslavle rdltytrsst
      121 vlsvggirqq fslpeniqls mfsaeflrnv kqnlsvldfd ppdirfqphg ylflaseegv
      181 dqmlknaelq edlgakiell kpdslrqrfp wlntegisla shglenegwf dpyalltalr
      241 rkavslgary lnaelvgfdf qethgyeael pnpvnhalvk tpdgvthkit failvvaagp
      301 hsgevakllr igagsgilgt alpleprkry vyvvhcpdgp glnfplvvap dgvycrregl
      361 gghfvcgksp apeeepevgd ldvdysffdk aiwptlaeri pafnslkvkt awagfydynt
      421 ldqngcvgrh paynnvyfat gfsghgiqma pgvgraimel ildgkyisid lerfglerfi
      481 chqpikeavi l
//
LOCUS       XP_077505093             205 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114938 [Amblyomma americanum].
ACCESSION   XP_077505093
VERSION     XP_077505093.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648967.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..205
                     /product="uncharacterized protein LOC144114938"
                     /calculated_mol_wt=22665
     CDS             1..205
                     /gene="LOC144114938"
                     /coded_by="XM_077648967.1:125..742"
                     /db_xref="GeneID:144114938"
ORIGIN      
        1 margagqerg swamppqceq rnkktvrtsa lselvlprls sdrfgdlaln tqphpptwil
       61 lalppflpaa trlrmlsfav lrwprssqsa crhfgpsspc awlpqveahf qlrciasqet
      121 kflhfvsslp pdvaeelvdi islpdtarpf dmlkaaiiny ksvsecsklq qllsatelgd
      181 srpskllhhi rqllgdsaaq hdtll
//
LOCUS       XP_077505094            2484 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114939 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505094
VERSION     XP_077505094.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648968.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2484
                     /product="uncharacterized protein LOC144114939 isoform X1"
                     /calculated_mol_wt=264398
     Region          2..237
                     /region_name="BAT2_N"
                     /note="BAT2 N-terminus; pfam07001"
                     /db_xref="CDD:462063"
     Region          <998..>1355
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          1265..>1499
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          <2175..2442
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..2484
                     /gene="LOC144114939"
                     /coded_by="XM_077648968.1:231..7685"
                     /db_xref="GeneID:144114939"
ORIGIN      
        1 mstvpglpsk gekgkpkyla ldinniykgk slenpkasaa pkhglqslgk vgagrrmppp
       61 vnlpslksen sgnnpnislv psggqgwgsa gkekgkspee nssgpqqqsq hsqsptshsq
      121 gqpaskqpqq qqqpppppqq qqqqppppqq pqpqpppppl ppvatstsap ggpssggtvg
      181 vgvgssagvk twssvtssqe gggpernflg hqspffpqef pklaggdvpi dgtqrsatds
      241 qygpgpslrp qmegtwsrgt lqqqqqqqpq qapggsggpg ggggqgcgpp gglppssgsg
      301 vngqasftpp prgalpsgvp pgggppsapa qpsyrqqavp pfvqqvygrg fpgnyapsys
      361 gvpggtrppy aysenrvrgv rnapdedgyq rpaiisekdl kgfdeilqnd sqdgwaaakg
      421 didynaklvf sddedsgssn rgagskaaei vpasesgkpg ptkgsekgec rpqgpagpkl
      481 sssrnrepgw dapsgqqpaq qraareppqt payldrderg rkawgapgsa elqpqpapls
      541 qqaqqappaq aapvrqwpqa qqaapptaap praagapple ylarstppaa tappamaaag
      601 fptgrvgppi pqqpshhtgf ggvgpvapsr gasrpgldql tgdeeelwrq rrqqqndema
      661 vtvertrqrr eeeekryeqi kqssaataaa ataanqqqqq pslpeesapv paekedldqr
      721 schssesree arggallpnr drpdfrqqss ggyqqnyqrp fknmpprflk kaemqrqqql
      781 rqqqqqqqqq qqqpqqpqqq qpqqqqqlqp qqqqpqqqpq qqqqqqpqqq pqasgglppp
      841 qgapsslsgm ggvsydprwv amgaggpylg qlgmvkplgs qrppesegst pyptaedrpy
      901 earearplec wppgagrpap pqadrpqqpl appmapphvp pggpydnwrp svapvyyggq
      961 vadfgrtpas ydqrlefppk rpdkeetpfd srsrgdsfrk erefedkqqr ekrenrdvmg
     1021 twsnareydq rlardkwere tqpsrpdsrd srasrdslrd eramppaeqr qdapmqqiep
     1081 hrlapervke kpqvpdwsdt pysaageskr rdwhthhppp vtqqqldsag pskknfvalr
     1141 rggvakdegk edskegkved hsrtdeeksk evakkddsek raatsrsrgg sggrfeanrg
     1201 rggrggngfg gvgyrgrsre yrrsrtergp rfdrkkdegs eessgveeek assrlrpkee
     1261 esegsdeacl dadrksrpgk esgrareprr deaknkeeks afsprgepsr rgrggavslg
     1321 rsargrypag gygpptskap fgkpeegskd eeankevsqg skqrsnssrp vvrrpnrmep
     1381 lpprfqknqs rrqdnpdrnk grggrgagpn kdalsdhgne dwetasessd vadrrgpeeg
     1441 vrqqpsgskk npssvpkasg gqerrrpgge arrgtggppg drngrgsvqr rdsvgggppa
     1501 rmgrgsgsrp ggrarqqrpg evtakgepsp aalvyrmdev klqdpggvqa altdlsarkh
     1561 tkkpeqgqqs grldkekpna lqgidisnya svvviddhpa dpslcdgddg gfqevtskkr
     1621 nricadgdsk kkkearskan vhgrqnklpp rlikkresdr misskaesql pakseagatd
     1681 smevastkah qhstfstkel gatgvirpiv nawdkpitha lrggsppssa pqpvttpavs
     1741 tadvlvssks ssfdrssdqh dsgidvsdqp asaassqrss psndgkppll gtsskglpam
     1801 pvgvpasaga vvvdsskpmg tvifenknfk aenaaldkfe sqtksqepgs tnapmlapva
     1861 skassdtvea skpkgedsgd mkldftfdse laqfgdekqa khmaiarsls seitsanspi
     1921 spstdelnlk iasvkkvwen vgplptvleh gavsvsgddt gsaavqapsf gsfggsgssd
     1981 nsfatsstat eamaqqgqae ptqvtsettf gsvsalkaap sslshhvasv vgtghqhpsa
     2041 snqhhhhhhh qqqqqqpvqq qqqlamaahh hsqaaqahgs lqahqqqqam mrqphqahsm
     2101 leqqnlskvr alhalsppps aaasslagld vramanlqva gmpqavaspp aallftstqq
     2161 mtqsaalyqt llqdpvsrpv ahqfsqathg ypappplgsy qgfnqggmfv ppppthsgpd
     2221 mftatgaalr lqptyqagpp vptsqvlgqq slpppaqqll ssgraapgga plgtsyypsq
     2281 ggyypsqqqg qalafgsqpt mhrtfhpppf kslelapdfg ttpppkaqaa kafppgspfa
     2341 fgqgrqaapp taagtgtppa ggamrppmhv lpmppnmirs appppsaptk ypgpiqrpqq
     2401 gltltstqqp rgppalqlra qqpparqlpt sdqqakqqae avkqaqlffa qskptapepa
     2461 vedglvvnds sddkavppke aaee
//
LOCUS       XP_077505095            2483 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114939 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505095
VERSION     XP_077505095.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648969.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2483
                     /product="uncharacterized protein LOC144114939 isoform X2"
                     /calculated_mol_wt=264270
     Region          2..237
                     /region_name="BAT2_N"
                     /note="BAT2 N-terminus; pfam07001"
                     /db_xref="CDD:462063"
     Region          <997..>1354
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          1264..>1498
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          <2174..2441
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..2483
                     /gene="LOC144114939"
                     /coded_by="XM_077648969.1:231..7682"
                     /db_xref="GeneID:144114939"
ORIGIN      
        1 mstvpglpsk gekgkpkyla ldinniykgk slenpkasaa pkhglqslgk vgagrrmppp
       61 vnlpslksen sgnnpnislv psggqgwgsa gkekgkspee nssgpqqqsq hsqsptshsq
      121 gqpaskqpqq qqqpppppqq qqqqppppqq pqpqpppppl ppvatstsap ggpssggtvg
      181 vgvgssagvk twssvtssqe gggpernflg hqspffpqef pklaggdvpi dgtqrsatds
      241 qygpgpslrp qmegtwsrgt lqqqqqqqpq qapggsggpg ggggqgcgpp gglppssgsg
      301 vngqasftpp prgalpsgvp pgggppsapa qpsyrqqavp pfvqqvygrg fpgnyapsys
      361 gvpggtrppy aysenrvrgv rnapdedgyq rpaiisekdl kgfdeilqnd sqdgwaaakg
      421 didynaklvf sddedsgssn rgagskaaei vpasesgkpg ptkgsekgec rpqgpagpkl
      481 sssrnrepgw dapsgqqpaq qraareppqt payldrderg rkawgapgsa elqpqpapls
      541 qqaqqappaq aapvrqwpqa qqaapptaap praagapple ylarstppaa tappamaaag
      601 fptgrvgppi pqpshhtgfg gvgpvapsrg asrpgldqlt gdeeelwrqr rqqqndemav
      661 tvertrqrre eeekryeqik qssaataaaa taanqqqqqp slpeesapvp aekedldqrs
      721 chssesreea rggallpnrd rpdfrqqssg gyqqnyqrpf knmpprflkk aemqrqqqlr
      781 qqqqqqqqqq qqpqqpqqqq pqqqqqlqpq qqqpqqqpqq qqqqqpqqqp qasgglpppq
      841 gapsslsgmg gvsydprwva mgaggpylgq lgmvkplgsq rppesegstp yptaedrpye
      901 arearplecw ppgagrpapp qadrpqqpla ppmapphvpp ggpydnwrps vapvyyggqv
      961 adfgrtpasy dqrlefppkr pdkeetpfds rsrgdsfrke refedkqqre krenrdvmgt
     1021 wsnareydqr lardkweret qpsrpdsrds rasrdslrde ramppaeqrq dapmqqieph
     1081 rlapervkek pqvpdwsdtp ysaageskrr dwhthhpppv tqqqldsagp skknfvalrr
     1141 ggvakdegke dskegkvedh srtdeekske vakkddsekr aatsrsrggs ggrfeanrgr
     1201 ggrggngfgg vgyrgrsrey rrsrtergpr fdrkkdegse essgveeeka ssrlrpkeee
     1261 segsdeacld adrksrpgke sgrareprrd eaknkeeksa fsprgepsrr grggavslgr
     1321 sargrypagg ygpptskapf gkpeegskde eankevsqgs kqrsnssrpv vrrpnrmepl
     1381 pprfqknqsr rqdnpdrnkg rggrgagpnk dalsdhgned wetasessdv adrrgpeegv
     1441 rqqpsgskkn pssvpkasgg qerrrpggea rrgtggppgd rngrgsvqrr dsvgggppar
     1501 mgrgsgsrpg grarqqrpge vtakgepspa alvyrmdevk lqdpggvqaa ltdlsarkht
     1561 kkpeqgqqsg rldkekpnal qgidisnyas vvviddhpad pslcdgddgg fqevtskkrn
     1621 ricadgdskk kkearskanv hgrqnklppr likkresdrm isskaesqlp akseagatds
     1681 mevastkahq hstfstkelg atgvirpivn awdkpithal rggsppssap qpvttpavst
     1741 advlvsskss sfdrssdqhd sgidvsdqpa saassqrssp sndgkppllg tsskglpamp
     1801 vgvpasagav vvdsskpmgt vifenknfka enaaldkfes qtksqepgst napmlapvas
     1861 kassdtveas kpkgedsgdm kldftfdsel aqfgdekqak hmaiarslss eitsanspis
     1921 pstdelnlki asvkkvwenv gplptvlehg avsvsgddtg saavqapsfg sfggsgssdn
     1981 sfatsstate amaqqgqaep tqvtsettfg svsalkaaps slshhvasvv gtghqhpsas
     2041 nqhhhhhhhq qqqqqpvqqq qqlamaahhh sqaaqahgsl qahqqqqamm rqphqahsml
     2101 eqqnlskvra lhalspppsa aasslagldv ramanlqvag mpqavasppa allftstqqm
     2161 tqsaalyqtl lqdpvsrpva hqfsqathgy pappplgsyq gfnqggmfvp pppthsgpdm
     2221 ftatgaalrl qptyqagppv ptsqvlgqqs lpppaqqlls sgraapggap lgtsyypsqg
     2281 gyypsqqqgq alafgsqptm hrtfhpppfk slelapdfgt tpppkaqaak afppgspfaf
     2341 gqgrqaappt aagtgtppag gamrppmhvl pmppnmirsa ppppsaptky pgpiqrpqqg
     2401 ltltstqqpr gppalqlraq qpparqlpts dqqakqqaea vkqaqlffaq skptapepav
     2461 edglvvndss ddkavppkea aee
//
LOCUS       XP_077505096            2483 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114939 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505096
VERSION     XP_077505096.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648970.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2483
                     /product="uncharacterized protein LOC144114939 isoform X3"
                     /calculated_mol_wt=264270
     Region          2..237
                     /region_name="BAT2_N"
                     /note="BAT2 N-terminus; pfam07001"
                     /db_xref="CDD:462063"
     Region          <997..>1354
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          1264..>1498
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          <2174..2441
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..2483
                     /gene="LOC144114939"
                     /coded_by="XM_077648970.1:237..7688"
                     /db_xref="GeneID:144114939"
ORIGIN      
        1 mstvpglpsk gekgkpkyla ldinniykgk slenpkasaa pkhglqslgk vgagrrmppp
       61 vnlpslksen sgnnpnislv psggqgwgsa gkekgkspee nssgpqqqsq hsqsptshsq
      121 gqpaskqpqq qqqpppppqq qqqqppppqq pqpqpppppl ppvatstsap ggpssggtvg
      181 vgvgssagvk twssvtssqe gggpernflg hqspffpqef pklaggdvpi dgtqrsatds
      241 qygpgpslrp qmegtwsrgt lqqqqqqqpq qapggsggpg ggggqgcgpp gglppssgsg
      301 vngqasftpp prgalpsgvp pgggppsapa qpsyrqqavp pfvqqvygrg fpgnyapsys
      361 gvpggtrppy aysenrvrgv rnapdedgyq rpaiisekdl kgfdeilqnd sqdgwaaakg
      421 didynaklvf sddedsgssn rgagskaaei vpasesgkpg ptkgsekgec rpqgpagpkl
      481 sssrnrepgw dapsgqqpaq qraareppqt payldrderg rkawgapgsa elqpqpapls
      541 qqaqqappaq aapvrqwpqa qqaapptaap praagapple ylarstppaa tappamaaag
      601 fptgrvgppi pqqpshhtgf ggvgpvapsr gasrpgldql tgdeeelwrq rrqqqndema
      661 vtvertrqrr eeeekryeqi kqssaataaa ataanqqqqq pslpeesapv paekedldqr
      721 schssesree arggallpnr drpdfrqqss ggyqnyqrpf knmpprflkk aemqrqqqlr
      781 qqqqqqqqqq qqpqqpqqqq pqqqqqlqpq qqqpqqqpqq qqqqqpqqqp qasgglpppq
      841 gapsslsgmg gvsydprwva mgaggpylgq lgmvkplgsq rppesegstp yptaedrpye
      901 arearplecw ppgagrpapp qadrpqqpla ppmapphvpp ggpydnwrps vapvyyggqv
      961 adfgrtpasy dqrlefppkr pdkeetpfds rsrgdsfrke refedkqqre krenrdvmgt
     1021 wsnareydqr lardkweret qpsrpdsrds rasrdslrde ramppaeqrq dapmqqieph
     1081 rlapervkek pqvpdwsdtp ysaageskrr dwhthhpppv tqqqldsagp skknfvalrr
     1141 ggvakdegke dskegkvedh srtdeekske vakkddsekr aatsrsrggs ggrfeanrgr
     1201 ggrggngfgg vgyrgrsrey rrsrtergpr fdrkkdegse essgveeeka ssrlrpkeee
     1261 segsdeacld adrksrpgke sgrareprrd eaknkeeksa fsprgepsrr grggavslgr
     1321 sargrypagg ygpptskapf gkpeegskde eankevsqgs kqrsnssrpv vrrpnrmepl
     1381 pprfqknqsr rqdnpdrnkg rggrgagpnk dalsdhgned wetasessdv adrrgpeegv
     1441 rqqpsgskkn pssvpkasgg qerrrpggea rrgtggppgd rngrgsvqrr dsvgggppar
     1501 mgrgsgsrpg grarqqrpge vtakgepspa alvyrmdevk lqdpggvqaa ltdlsarkht
     1561 kkpeqgqqsg rldkekpnal qgidisnyas vvviddhpad pslcdgddgg fqevtskkrn
     1621 ricadgdskk kkearskanv hgrqnklppr likkresdrm isskaesqlp akseagatds
     1681 mevastkahq hstfstkelg atgvirpivn awdkpithal rggsppssap qpvttpavst
     1741 advlvsskss sfdrssdqhd sgidvsdqpa saassqrssp sndgkppllg tsskglpamp
     1801 vgvpasagav vvdsskpmgt vifenknfka enaaldkfes qtksqepgst napmlapvas
     1861 kassdtveas kpkgedsgdm kldftfdsel aqfgdekqak hmaiarslss eitsanspis
     1921 pstdelnlki asvkkvwenv gplptvlehg avsvsgddtg saavqapsfg sfggsgssdn
     1981 sfatsstate amaqqgqaep tqvtsettfg svsalkaaps slshhvasvv gtghqhpsas
     2041 nqhhhhhhhq qqqqqpvqqq qqlamaahhh sqaaqahgsl qahqqqqamm rqphqahsml
     2101 eqqnlskvra lhalspppsa aasslagldv ramanlqvag mpqavasppa allftstqqm
     2161 tqsaalyqtl lqdpvsrpva hqfsqathgy pappplgsyq gfnqggmfvp pppthsgpdm
     2221 ftatgaalrl qptyqagppv ptsqvlgqqs lpppaqqlls sgraapggap lgtsyypsqg
     2281 gyypsqqqgq alafgsqptm hrtfhpppfk slelapdfgt tpppkaqaak afppgspfaf
     2341 gqgrqaappt aagtgtppag gamrppmhvl pmppnmirsa ppppsaptky pgpiqrpqqg
     2401 ltltstqqpr gppalqlraq qpparqlpts dqqakqqaea vkqaqlffaq skptapepav
     2461 edglvvndss ddkavppkea aee
//
LOCUS       XP_077505097            2483 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114939 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505097
VERSION     XP_077505097.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648971.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2483
                     /product="uncharacterized protein LOC144114939 isoform X4"
                     /calculated_mol_wt=264311
     Region          2..236
                     /region_name="BAT2_N"
                     /note="BAT2 N-terminus; pfam07001"
                     /db_xref="CDD:462063"
     Region          <997..>1354
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          1264..>1498
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          <2174..2441
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..2483
                     /gene="LOC144114939"
                     /coded_by="XM_077648971.1:231..7682"
                     /db_xref="GeneID:144114939"
ORIGIN      
        1 mstvpglpsk gekgkpkyla ldinniykgk slenpkasaa pkhglqslgk vgagrrmppp
       61 vnlpslksen sgnnpnislv psggqgwgsa gkekgkspee nsgpqqqsqh sqsptshsqg
      121 qpaskqpqqq qqpppppqqq qqqppppqqp qpqppppplp pvatstsapg gpssggtvgv
      181 gvgssagvkt wssvtssqeg ggpernflgh qspffpqefp klaggdvpid gtqrsatdsq
      241 ygpgpslrpq megtwsrgtl qqqqqqqpqq apggsggpgg gggqgcgppg glppssgsgv
      301 ngqasftppp rgalpsgvpp gggppsapaq psyrqqavpp fvqqvygrgf pgnyapsysg
      361 vpggtrppya ysenrvrgvr napdedgyqr paiisekdlk gfdeilqnds qdgwaaakgd
      421 idynaklvfs ddedsgssnr gagskaaeiv pasesgkpgp tkgsekgecr pqgpagpkls
      481 ssrnrepgwd apsgqqpaqq raareppqtp ayldrdergr kawgapgsae lqpqpaplsq
      541 qaqqappaqa apvrqwpqaq qaapptaapp raagappley larstppaat appamaaagf
      601 ptgrvgppip qqpshhtgfg gvgpvapsrg asrpgldqlt gdeeelwrqr rqqqndemav
      661 tvertrqrre eeekryeqik qssaataaaa taanqqqqqp slpeesapvp aekedldqrs
      721 chssesreea rggallpnrd rpdfrqqssg gyqqnyqrpf knmpprflkk aemqrqqqlr
      781 qqqqqqqqqq qqpqqpqqqq pqqqqqlqpq qqqpqqqpqq qqqqqpqqqp qasgglpppq
      841 gapsslsgmg gvsydprwva mgaggpylgq lgmvkplgsq rppesegstp yptaedrpye
      901 arearplecw ppgagrpapp qadrpqqpla ppmapphvpp ggpydnwrps vapvyyggqv
      961 adfgrtpasy dqrlefppkr pdkeetpfds rsrgdsfrke refedkqqre krenrdvmgt
     1021 wsnareydqr lardkweret qpsrpdsrds rasrdslrde ramppaeqrq dapmqqieph
     1081 rlapervkek pqvpdwsdtp ysaageskrr dwhthhpppv tqqqldsagp skknfvalrr
     1141 ggvakdegke dskegkvedh srtdeekske vakkddsekr aatsrsrggs ggrfeanrgr
     1201 ggrggngfgg vgyrgrsrey rrsrtergpr fdrkkdegse essgveeeka ssrlrpkeee
     1261 segsdeacld adrksrpgke sgrareprrd eaknkeeksa fsprgepsrr grggavslgr
     1321 sargrypagg ygpptskapf gkpeegskde eankevsqgs kqrsnssrpv vrrpnrmepl
     1381 pprfqknqsr rqdnpdrnkg rggrgagpnk dalsdhgned wetasessdv adrrgpeegv
     1441 rqqpsgskkn pssvpkasgg qerrrpggea rrgtggppgd rngrgsvqrr dsvgggppar
     1501 mgrgsgsrpg grarqqrpge vtakgepspa alvyrmdevk lqdpggvqaa ltdlsarkht
     1561 kkpeqgqqsg rldkekpnal qgidisnyas vvviddhpad pslcdgddgg fqevtskkrn
     1621 ricadgdskk kkearskanv hgrqnklppr likkresdrm isskaesqlp akseagatds
     1681 mevastkahq hstfstkelg atgvirpivn awdkpithal rggsppssap qpvttpavst
     1741 advlvsskss sfdrssdqhd sgidvsdqpa saassqrssp sndgkppllg tsskglpamp
     1801 vgvpasagav vvdsskpmgt vifenknfka enaaldkfes qtksqepgst napmlapvas
     1861 kassdtveas kpkgedsgdm kldftfdsel aqfgdekqak hmaiarslss eitsanspis
     1921 pstdelnlki asvkkvwenv gplptvlehg avsvsgddtg saavqapsfg sfggsgssdn
     1981 sfatsstate amaqqgqaep tqvtsettfg svsalkaaps slshhvasvv gtghqhpsas
     2041 nqhhhhhhhq qqqqqpvqqq qqlamaahhh sqaaqahgsl qahqqqqamm rqphqahsml
     2101 eqqnlskvra lhalspppsa aasslagldv ramanlqvag mpqavasppa allftstqqm
     2161 tqsaalyqtl lqdpvsrpva hqfsqathgy pappplgsyq gfnqggmfvp pppthsgpdm
     2221 ftatgaalrl qptyqagppv ptsqvlgqqs lpppaqqlls sgraapggap lgtsyypsqg
     2281 gyypsqqqgq alafgsqptm hrtfhpppfk slelapdfgt tpppkaqaak afppgspfaf
     2341 gqgrqaappt aagtgtppag gamrppmhvl pmppnmirsa ppppsaptky pgpiqrpqqg
     2401 ltltstqqpr gppalqlraq qpparqlpts dqqakqqaea vkqaqlffaq skptapepav
     2461 edglvvndss ddkavppkea aee
//
LOCUS       XP_077505098            2482 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114939 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077505098
VERSION     XP_077505098.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648972.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2482
                     /product="uncharacterized protein LOC144114939 isoform X5"
                     /calculated_mol_wt=264142
     Region          2..237
                     /region_name="BAT2_N"
                     /note="BAT2 N-terminus; pfam07001"
                     /db_xref="CDD:462063"
     Region          <996..>1353
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          1263..>1497
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          <2173..2440
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..2482
                     /gene="LOC144114939"
                     /coded_by="XM_077648972.1:239..7687"
                     /db_xref="GeneID:144114939"
ORIGIN      
        1 mstvpglpsk gekgkpkyla ldinniykgk slenpkasaa pkhglqslgk vgagrrmppp
       61 vnlpslksen sgnnpnislv psggqgwgsa gkekgkspee nssgpqqqsq hsqsptshsq
      121 gqpaskqpqq qqqpppppqq qqqqppppqq pqpqpppppl ppvatstsap ggpssggtvg
      181 vgvgssagvk twssvtssqe gggpernflg hqspffpqef pklaggdvpi dgtqrsatds
      241 qygpgpslrp qmegtwsrgt lqqqqqqqpq qapggsggpg ggggqgcgpp gglppssgsg
      301 vngqasftpp prgalpsgvp pgggppsapa qpsyrqqavp pfvqqvygrg fpgnyapsys
      361 gvpggtrppy aysenrvrgv rnapdedgyq rpaiisekdl kgfdeilqnd sqdgwaaakg
      421 didynaklvf sddedsgssn rgagskaaei vpasesgkpg ptkgsekgec rpqgpagpkl
      481 sssrnrepgw dapsgqqpaq qraareppqt payldrderg rkawgapgsa elqpqpapls
      541 qqaqqappaq aapvrqwpqa qqaapptaap praagapple ylarstppaa tappamaaag
      601 fptgrvgppi pqpshhtgfg gvgpvapsrg asrpgldqlt gdeeelwrqr rqqqndemav
      661 tvertrqrre eeekryeqik qssaataaaa taanqqqqqp slpeesapvp aekedldqrs
      721 chssesreea rggallpnrd rpdfrqqssg gyqnyqrpfk nmpprflkka emqrqqqlrq
      781 qqqqqqqqqq qpqqpqqqqp qqqqqlqpqq qqpqqqpqqq qqqqpqqqpq asgglpppqg
      841 apsslsgmgg vsydprwvam gaggpylgql gmvkplgsqr ppesegstpy ptaedrpyea
      901 rearplecwp pgagrpappq adrpqqplap pmapphvppg gpydnwrpsv apvyyggqva
      961 dfgrtpasyd qrlefppkrp dkeetpfdsr srgdsfrker efedkqqrek renrdvmgtw
     1021 snareydqrl ardkweretq psrpdsrdsr asrdslrder amppaeqrqd apmqqiephr
     1081 lapervkekp qvpdwsdtpy saageskrrd whthhpppvt qqqldsagps kknfvalrrg
     1141 gvakdegked skegkvedhs rtdeekskev akkddsekra atsrsrggsg grfeanrgrg
     1201 grggngfggv gyrgrsreyr rsrtergprf drkkdegsee ssgveeekas srlrpkeees
     1261 egsdeaclda drksrpgkes grareprrde aknkeeksaf sprgepsrrg rggavslgrs
     1321 argrypaggy gpptskapfg kpeegskdee ankevsqgsk qrsnssrpvv rrpnrmeplp
     1381 prfqknqsrr qdnpdrnkgr ggrgagpnkd alsdhgnedw etasessdva drrgpeegvr
     1441 qqpsgskknp ssvpkasggq errrpggear rgtggppgdr ngrgsvqrrd svgggpparm
     1501 grgsgsrpgg rarqqrpgev takgepspaa lvyrmdevkl qdpggvqaal tdlsarkhtk
     1561 kpeqgqqsgr ldkekpnalq gidisnyasv vviddhpadp slcdgddggf qevtskkrnr
     1621 icadgdskkk kearskanvh grqnklpprl ikkresdrmi sskaesqlpa kseagatdsm
     1681 evastkahqh stfstkelga tgvirpivna wdkpithalr ggsppssapq pvttpavsta
     1741 dvlvssksss fdrssdqhds gidvsdqpas aassqrssps ndgkppllgt sskglpampv
     1801 gvpasagavv vdsskpmgtv ifenknfkae naaldkfesq tksqepgstn apmlapvask
     1861 assdtveask pkgedsgdmk ldftfdsela qfgdekqakh maiarslsse itsanspisp
     1921 stdelnlkia svkkvwenvg plptvlehga vsvsgddtgs aavqapsfgs fggsgssdns
     1981 fatsstatea maqqgqaept qvtsettfgs vsalkaapss lshhvasvvg tghqhpsasn
     2041 qhhhhhhhqq qqqqpvqqqq qlamaahhhs qaaqahgslq ahqqqqammr qphqahsmle
     2101 qqnlskvral halspppsaa asslagldvr amanlqvagm pqavasppaa llftstqqmt
     2161 qsaalyqtll qdpvsrpvah qfsqathgyp appplgsyqg fnqggmfvpp ppthsgpdmf
     2221 tatgaalrlq ptyqagppvp tsqvlgqqsl pppaqqllss graapggapl gtsyypsqgg
     2281 yypsqqqgqa lafgsqptmh rtfhpppfks lelapdfgtt pppkaqaaka fppgspfafg
     2341 qgrqaappta agtgtppagg amrppmhvlp mppnmirsap pppsaptkyp gpiqrpqqgl
     2401 tltstqqprg ppalqlraqq pparqlptsd qqakqqaeav kqaqlffaqs kptapepave
     2461 dglvvndssd dkavppkeaa ee
//
LOCUS       XP_077505099            2447 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114939 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077505099
VERSION     XP_077505099.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648973.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2447
                     /product="uncharacterized protein LOC144114939 isoform X6"
                     /calculated_mol_wt=260356
     Region          2..237
                     /region_name="BAT2_N"
                     /note="BAT2 N-terminus; pfam07001"
                     /db_xref="CDD:462063"
     Region          <998..>1355
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          1265..>1499
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          <2138..2405
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..2447
                     /gene="LOC144114939"
                     /coded_by="XM_077648973.1:231..7574"
                     /db_xref="GeneID:144114939"
ORIGIN      
        1 mstvpglpsk gekgkpkyla ldinniykgk slenpkasaa pkhglqslgk vgagrrmppp
       61 vnlpslksen sgnnpnislv psggqgwgsa gkekgkspee nssgpqqqsq hsqsptshsq
      121 gqpaskqpqq qqqpppppqq qqqqppppqq pqpqpppppl ppvatstsap ggpssggtvg
      181 vgvgssagvk twssvtssqe gggpernflg hqspffpqef pklaggdvpi dgtqrsatds
      241 qygpgpslrp qmegtwsrgt lqqqqqqqpq qapggsggpg ggggqgcgpp gglppssgsg
      301 vngqasftpp prgalpsgvp pgggppsapa qpsyrqqavp pfvqqvygrg fpgnyapsys
      361 gvpggtrppy aysenrvrgv rnapdedgyq rpaiisekdl kgfdeilqnd sqdgwaaakg
      421 didynaklvf sddedsgssn rgagskaaei vpasesgkpg ptkgsekgec rpqgpagpkl
      481 sssrnrepgw dapsgqqpaq qraareppqt payldrderg rkawgapgsa elqpqpapls
      541 qqaqqappaq aapvrqwpqa qqaapptaap praagapple ylarstppaa tappamaaag
      601 fptgrvgppi pqqpshhtgf ggvgpvapsr gasrpgldql tgdeeelwrq rrqqqndema
      661 vtvertrqrr eeeekryeqi kqssaataaa ataanqqqqq pslpeesapv paekedldqr
      721 schssesree arggallpnr drpdfrqqss ggyqqnyqrp fknmpprflk kaemqrqqql
      781 rqqqqqqqqq qqqpqqpqqq qpqqqqqlqp qqqqpqqqpq qqqqqqpqqq pqasgglppp
      841 qgapsslsgm ggvsydprwv amgaggpylg qlgmvkplgs qrppesegst pyptaedrpy
      901 earearplec wppgagrpap pqadrpqqpl appmapphvp pggpydnwrp svapvyyggq
      961 vadfgrtpas ydqrlefppk rpdkeetpfd srsrgdsfrk erefedkqqr ekrenrdvmg
     1021 twsnareydq rlardkwere tqpsrpdsrd srasrdslrd eramppaeqr qdapmqqiep
     1081 hrlapervke kpqvpdwsdt pysaageskr rdwhthhppp vtqqqldsag pskknfvalr
     1141 rggvakdegk edskegkved hsrtdeeksk evakkddsek raatsrsrgg sggrfeanrg
     1201 rggrggngfg gvgyrgrsre yrrsrtergp rfdrkkdegs eessgveeek assrlrpkee
     1261 esegsdeacl dadrksrpgk esgrareprr deaknkeeks afsprgepsr rgrggavslg
     1321 rsargrypag gygpptskap fgkpeegskd eeankevsqg skqrsnssrp vvrrpnrmep
     1381 lpprfqknqs rrqdnpdrnk grggrgagpn kdalsdhgne dwetasessd vadrrgpeeg
     1441 vrqqpsgskk npssvpkasg gqerrrpgge arrgtggppg drngrgsvqr rdsvgggppa
     1501 rmgrgsgsrp ggrarqqrpg evtakgepsp aalvyrmdev klqdpggvqa altdlsarkh
     1561 tkkpeqgqqs grldkekpna lqgidisnya svvviddhpa dpslcdgddg gfqevtskkr
     1621 nricadgdsk kkkearskan vhgrqnklpp rlikkresdr misskaesql pakseagatd
     1681 smevastkah qhstfstkel gatgvirpiv nawdkpitha lrggsppssa pqpvttpavs
     1741 tadvlvssks ssfdrssdqh dsgidvsdqp asaassqrss psndgkppll gtsskglpam
     1801 pvgvpasage pgstnapmla pvaskassdt veaskpkged sgdmkldftf dselaqfgde
     1861 kqakhmaiar slsseitsan spispstdel nlkiasvkkv wenvgplptv lehgavsvsg
     1921 ddtgsaavqa psfgsfggsg ssdnsfatss tateamaqqg qaeptqvtse ttfgsvsalk
     1981 aapsslshhv asvvgtghqh psasnqhhhh hhhqqqqqqp vqqqqqlama ahhhsqaaqa
     2041 hgslqahqqq qammrqphqa hsmleqqnls kvralhalsp ppsaaassla gldvramanl
     2101 qvagmpqava sppaallfts tqqmtqsaal yqtllqdpvs rpvahqfsqa thgypapppl
     2161 gsyqgfnqgg mfvppppths gpdmftatga alrlqptyqa gppvptsqvl gqqslpppaq
     2221 qllssgraap ggaplgtsyy psqggyypsq qqgqalafgs qptmhrtfhp ppfkslelap
     2281 dfgttpppka qaakafppgs pfafgqgrqa apptaagtgt ppaggamrpp mhvlpmppnm
     2341 irsappppsa ptkypgpiqr pqqgltltst qqprgppalq lraqqpparq lptsdqqakq
     2401 qaeavkqaql ffaqskptap epavedglvv ndssddkavp pkeaaee
//
LOCUS       XP_077505100            2446 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114939 isoform X7 [Amblyomma
            americanum].
ACCESSION   XP_077505100
VERSION     XP_077505100.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648974.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2446
                     /product="uncharacterized protein LOC144114939 isoform X7"
                     /calculated_mol_wt=260228
     Region          2..237
                     /region_name="BAT2_N"
                     /note="BAT2 N-terminus; pfam07001"
                     /db_xref="CDD:462063"
     Region          <997..>1354
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          1264..>1498
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          <2137..2404
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..2446
                     /gene="LOC144114939"
                     /coded_by="XM_077648974.1:231..7571"
                     /db_xref="GeneID:144114939"
ORIGIN      
        1 mstvpglpsk gekgkpkyla ldinniykgk slenpkasaa pkhglqslgk vgagrrmppp
       61 vnlpslksen sgnnpnislv psggqgwgsa gkekgkspee nssgpqqqsq hsqsptshsq
      121 gqpaskqpqq qqqpppppqq qqqqppppqq pqpqpppppl ppvatstsap ggpssggtvg
      181 vgvgssagvk twssvtssqe gggpernflg hqspffpqef pklaggdvpi dgtqrsatds
      241 qygpgpslrp qmegtwsrgt lqqqqqqqpq qapggsggpg ggggqgcgpp gglppssgsg
      301 vngqasftpp prgalpsgvp pgggppsapa qpsyrqqavp pfvqqvygrg fpgnyapsys
      361 gvpggtrppy aysenrvrgv rnapdedgyq rpaiisekdl kgfdeilqnd sqdgwaaakg
      421 didynaklvf sddedsgssn rgagskaaei vpasesgkpg ptkgsekgec rpqgpagpkl
      481 sssrnrepgw dapsgqqpaq qraareppqt payldrderg rkawgapgsa elqpqpapls
      541 qqaqqappaq aapvrqwpqa qqaapptaap praagapple ylarstppaa tappamaaag
      601 fptgrvgppi pqpshhtgfg gvgpvapsrg asrpgldqlt gdeeelwrqr rqqqndemav
      661 tvertrqrre eeekryeqik qssaataaaa taanqqqqqp slpeesapvp aekedldqrs
      721 chssesreea rggallpnrd rpdfrqqssg gyqqnyqrpf knmpprflkk aemqrqqqlr
      781 qqqqqqqqqq qqpqqpqqqq pqqqqqlqpq qqqpqqqpqq qqqqqpqqqp qasgglpppq
      841 gapsslsgmg gvsydprwva mgaggpylgq lgmvkplgsq rppesegstp yptaedrpye
      901 arearplecw ppgagrpapp qadrpqqpla ppmapphvpp ggpydnwrps vapvyyggqv
      961 adfgrtpasy dqrlefppkr pdkeetpfds rsrgdsfrke refedkqqre krenrdvmgt
     1021 wsnareydqr lardkweret qpsrpdsrds rasrdslrde ramppaeqrq dapmqqieph
     1081 rlapervkek pqvpdwsdtp ysaageskrr dwhthhpppv tqqqldsagp skknfvalrr
     1141 ggvakdegke dskegkvedh srtdeekske vakkddsekr aatsrsrggs ggrfeanrgr
     1201 ggrggngfgg vgyrgrsrey rrsrtergpr fdrkkdegse essgveeeka ssrlrpkeee
     1261 segsdeacld adrksrpgke sgrareprrd eaknkeeksa fsprgepsrr grggavslgr
     1321 sargrypagg ygpptskapf gkpeegskde eankevsqgs kqrsnssrpv vrrpnrmepl
     1381 pprfqknqsr rqdnpdrnkg rggrgagpnk dalsdhgned wetasessdv adrrgpeegv
     1441 rqqpsgskkn pssvpkasgg qerrrpggea rrgtggppgd rngrgsvqrr dsvgggppar
     1501 mgrgsgsrpg grarqqrpge vtakgepspa alvyrmdevk lqdpggvqaa ltdlsarkht
     1561 kkpeqgqqsg rldkekpnal qgidisnyas vvviddhpad pslcdgddgg fqevtskkrn
     1621 ricadgdskk kkearskanv hgrqnklppr likkresdrm isskaesqlp akseagatds
     1681 mevastkahq hstfstkelg atgvirpivn awdkpithal rggsppssap qpvttpavst
     1741 advlvsskss sfdrssdqhd sgidvsdqpa saassqrssp sndgkppllg tsskglpamp
     1801 vgvpasagep gstnapmlap vaskassdtv easkpkgeds gdmkldftfd selaqfgdek
     1861 qakhmaiars lsseitsans pispstdeln lkiasvkkvw envgplptvl ehgavsvsgd
     1921 dtgsaavqap sfgsfggsgs sdnsfatsst ateamaqqgq aeptqvtset tfgsvsalka
     1981 apsslshhva svvgtghqhp sasnqhhhhh hhqqqqqqpv qqqqqlamaa hhhsqaaqah
     2041 gslqahqqqq ammrqphqah smleqqnlsk vralhalspp psaaasslag ldvramanlq
     2101 vagmpqavas ppaallftst qqmtqsaaly qtllqdpvsr pvahqfsqat hgypappplg
     2161 syqgfnqggm fvppppthsg pdmftatgaa lrlqptyqag ppvptsqvlg qqslpppaqq
     2221 llssgraapg gaplgtsyyp sqggyypsqq qgqalafgsq ptmhrtfhpp pfkslelapd
     2281 fgttpppkaq aakafppgsp fafgqgrqaa pptaagtgtp paggamrppm hvlpmppnmi
     2341 rsappppsap tkypgpiqrp qqgltltstq qprgppalql raqqpparql ptsdqqakqq
     2401 aeavkqaqlf faqskptape pavedglvvn dssddkavpp keaaee
//
LOCUS       XP_077505101            2446 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114939 isoform X8 [Amblyomma
            americanum].
ACCESSION   XP_077505101
VERSION     XP_077505101.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648975.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2446
                     /product="uncharacterized protein LOC144114939 isoform X8"
                     /calculated_mol_wt=260228
     Region          2..237
                     /region_name="BAT2_N"
                     /note="BAT2 N-terminus; pfam07001"
                     /db_xref="CDD:462063"
     Region          <997..>1354
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          1264..>1498
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          <2137..2404
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..2446
                     /gene="LOC144114939"
                     /coded_by="XM_077648975.1:237..7577"
                     /db_xref="GeneID:144114939"
ORIGIN      
        1 mstvpglpsk gekgkpkyla ldinniykgk slenpkasaa pkhglqslgk vgagrrmppp
       61 vnlpslksen sgnnpnislv psggqgwgsa gkekgkspee nssgpqqqsq hsqsptshsq
      121 gqpaskqpqq qqqpppppqq qqqqppppqq pqpqpppppl ppvatstsap ggpssggtvg
      181 vgvgssagvk twssvtssqe gggpernflg hqspffpqef pklaggdvpi dgtqrsatds
      241 qygpgpslrp qmegtwsrgt lqqqqqqqpq qapggsggpg ggggqgcgpp gglppssgsg
      301 vngqasftpp prgalpsgvp pgggppsapa qpsyrqqavp pfvqqvygrg fpgnyapsys
      361 gvpggtrppy aysenrvrgv rnapdedgyq rpaiisekdl kgfdeilqnd sqdgwaaakg
      421 didynaklvf sddedsgssn rgagskaaei vpasesgkpg ptkgsekgec rpqgpagpkl
      481 sssrnrepgw dapsgqqpaq qraareppqt payldrderg rkawgapgsa elqpqpapls
      541 qqaqqappaq aapvrqwpqa qqaapptaap praagapple ylarstppaa tappamaaag
      601 fptgrvgppi pqqpshhtgf ggvgpvapsr gasrpgldql tgdeeelwrq rrqqqndema
      661 vtvertrqrr eeeekryeqi kqssaataaa ataanqqqqq pslpeesapv paekedldqr
      721 schssesree arggallpnr drpdfrqqss ggyqnyqrpf knmpprflkk aemqrqqqlr
      781 qqqqqqqqqq qqpqqpqqqq pqqqqqlqpq qqqpqqqpqq qqqqqpqqqp qasgglpppq
      841 gapsslsgmg gvsydprwva mgaggpylgq lgmvkplgsq rppesegstp yptaedrpye
      901 arearplecw ppgagrpapp qadrpqqpla ppmapphvpp ggpydnwrps vapvyyggqv
      961 adfgrtpasy dqrlefppkr pdkeetpfds rsrgdsfrke refedkqqre krenrdvmgt
     1021 wsnareydqr lardkweret qpsrpdsrds rasrdslrde ramppaeqrq dapmqqieph
     1081 rlapervkek pqvpdwsdtp ysaageskrr dwhthhpppv tqqqldsagp skknfvalrr
     1141 ggvakdegke dskegkvedh srtdeekske vakkddsekr aatsrsrggs ggrfeanrgr
     1201 ggrggngfgg vgyrgrsrey rrsrtergpr fdrkkdegse essgveeeka ssrlrpkeee
     1261 segsdeacld adrksrpgke sgrareprrd eaknkeeksa fsprgepsrr grggavslgr
     1321 sargrypagg ygpptskapf gkpeegskde eankevsqgs kqrsnssrpv vrrpnrmepl
     1381 pprfqknqsr rqdnpdrnkg rggrgagpnk dalsdhgned wetasessdv adrrgpeegv
     1441 rqqpsgskkn pssvpkasgg qerrrpggea rrgtggppgd rngrgsvqrr dsvgggppar
     1501 mgrgsgsrpg grarqqrpge vtakgepspa alvyrmdevk lqdpggvqaa ltdlsarkht
     1561 kkpeqgqqsg rldkekpnal qgidisnyas vvviddhpad pslcdgddgg fqevtskkrn
     1621 ricadgdskk kkearskanv hgrqnklppr likkresdrm isskaesqlp akseagatds
     1681 mevastkahq hstfstkelg atgvirpivn awdkpithal rggsppssap qpvttpavst
     1741 advlvsskss sfdrssdqhd sgidvsdqpa saassqrssp sndgkppllg tsskglpamp
     1801 vgvpasagep gstnapmlap vaskassdtv easkpkgeds gdmkldftfd selaqfgdek
     1861 qakhmaiars lsseitsans pispstdeln lkiasvkkvw envgplptvl ehgavsvsgd
     1921 dtgsaavqap sfgsfggsgs sdnsfatsst ateamaqqgq aeptqvtset tfgsvsalka
     1981 apsslshhva svvgtghqhp sasnqhhhhh hhqqqqqqpv qqqqqlamaa hhhsqaaqah
     2041 gslqahqqqq ammrqphqah smleqqnlsk vralhalspp psaaasslag ldvramanlq
     2101 vagmpqavas ppaallftst qqmtqsaaly qtllqdpvsr pvahqfsqat hgypappplg
     2161 syqgfnqggm fvppppthsg pdmftatgaa lrlqptyqag ppvptsqvlg qqslpppaqq
     2221 llssgraapg gaplgtsyyp sqggyypsqq qgqalafgsq ptmhrtfhpp pfkslelapd
     2281 fgttpppkaq aakafppgsp fafgqgrqaa pptaagtgtp paggamrppm hvlpmppnmi
     2341 rsappppsap tkypgpiqrp qqgltltstq qprgppalql raqqpparql ptsdqqakqq
     2401 aeavkqaqlf faqskptape pavedglvvn dssddkavpp keaaee
//
LOCUS       XP_077505102            2445 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114939 isoform X9 [Amblyomma
            americanum].
ACCESSION   XP_077505102
VERSION     XP_077505102.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648976.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2445
                     /product="uncharacterized protein LOC144114939 isoform X9"
                     /calculated_mol_wt=260100
     Region          2..237
                     /region_name="BAT2_N"
                     /note="BAT2 N-terminus; pfam07001"
                     /db_xref="CDD:462063"
     Region          <996..>1353
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          1263..>1497
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          <2136..2403
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..2445
                     /gene="LOC144114939"
                     /coded_by="XM_077648976.1:239..7576"
                     /db_xref="GeneID:144114939"
ORIGIN      
        1 mstvpglpsk gekgkpkyla ldinniykgk slenpkasaa pkhglqslgk vgagrrmppp
       61 vnlpslksen sgnnpnislv psggqgwgsa gkekgkspee nssgpqqqsq hsqsptshsq
      121 gqpaskqpqq qqqpppppqq qqqqppppqq pqpqpppppl ppvatstsap ggpssggtvg
      181 vgvgssagvk twssvtssqe gggpernflg hqspffpqef pklaggdvpi dgtqrsatds
      241 qygpgpslrp qmegtwsrgt lqqqqqqqpq qapggsggpg ggggqgcgpp gglppssgsg
      301 vngqasftpp prgalpsgvp pgggppsapa qpsyrqqavp pfvqqvygrg fpgnyapsys
      361 gvpggtrppy aysenrvrgv rnapdedgyq rpaiisekdl kgfdeilqnd sqdgwaaakg
      421 didynaklvf sddedsgssn rgagskaaei vpasesgkpg ptkgsekgec rpqgpagpkl
      481 sssrnrepgw dapsgqqpaq qraareppqt payldrderg rkawgapgsa elqpqpapls
      541 qqaqqappaq aapvrqwpqa qqaapptaap praagapple ylarstppaa tappamaaag
      601 fptgrvgppi pqpshhtgfg gvgpvapsrg asrpgldqlt gdeeelwrqr rqqqndemav
      661 tvertrqrre eeekryeqik qssaataaaa taanqqqqqp slpeesapvp aekedldqrs
      721 chssesreea rggallpnrd rpdfrqqssg gyqnyqrpfk nmpprflkka emqrqqqlrq
      781 qqqqqqqqqq qpqqpqqqqp qqqqqlqpqq qqpqqqpqqq qqqqpqqqpq asgglpppqg
      841 apsslsgmgg vsydprwvam gaggpylgql gmvkplgsqr ppesegstpy ptaedrpyea
      901 rearplecwp pgagrpappq adrpqqplap pmapphvppg gpydnwrpsv apvyyggqva
      961 dfgrtpasyd qrlefppkrp dkeetpfdsr srgdsfrker efedkqqrek renrdvmgtw
     1021 snareydqrl ardkweretq psrpdsrdsr asrdslrder amppaeqrqd apmqqiephr
     1081 lapervkekp qvpdwsdtpy saageskrrd whthhpppvt qqqldsagps kknfvalrrg
     1141 gvakdegked skegkvedhs rtdeekskev akkddsekra atsrsrggsg grfeanrgrg
     1201 grggngfggv gyrgrsreyr rsrtergprf drkkdegsee ssgveeekas srlrpkeees
     1261 egsdeaclda drksrpgkes grareprrde aknkeeksaf sprgepsrrg rggavslgrs
     1321 argrypaggy gpptskapfg kpeegskdee ankevsqgsk qrsnssrpvv rrpnrmeplp
     1381 prfqknqsrr qdnpdrnkgr ggrgagpnkd alsdhgnedw etasessdva drrgpeegvr
     1441 qqpsgskknp ssvpkasggq errrpggear rgtggppgdr ngrgsvqrrd svgggpparm
     1501 grgsgsrpgg rarqqrpgev takgepspaa lvyrmdevkl qdpggvqaal tdlsarkhtk
     1561 kpeqgqqsgr ldkekpnalq gidisnyasv vviddhpadp slcdgddggf qevtskkrnr
     1621 icadgdskkk kearskanvh grqnklpprl ikkresdrmi sskaesqlpa kseagatdsm
     1681 evastkahqh stfstkelga tgvirpivna wdkpithalr ggsppssapq pvttpavsta
     1741 dvlvssksss fdrssdqhds gidvsdqpas aassqrssps ndgkppllgt sskglpampv
     1801 gvpasagepg stnapmlapv askassdtve askpkgedsg dmkldftfds elaqfgdekq
     1861 akhmaiarsl sseitsansp ispstdelnl kiasvkkvwe nvgplptvle hgavsvsgdd
     1921 tgsaavqaps fgsfggsgss dnsfatssta teamaqqgqa eptqvtsett fgsvsalkaa
     1981 psslshhvas vvgtghqhps asnqhhhhhh hqqqqqqpvq qqqqlamaah hhsqaaqahg
     2041 slqahqqqqa mmrqphqahs mleqqnlskv ralhalsppp saaasslagl dvramanlqv
     2101 agmpqavasp paallftstq qmtqsaalyq tllqdpvsrp vahqfsqath gypappplgs
     2161 yqgfnqggmf vppppthsgp dmftatgaal rlqptyqagp pvptsqvlgq qslpppaqql
     2221 lssgraapgg aplgtsyyps qggyypsqqq gqalafgsqp tmhrtfhppp fkslelapdf
     2281 gttpppkaqa akafppgspf afgqgrqaap ptaagtgtpp aggamrppmh vlpmppnmir
     2341 sappppsapt kypgpiqrpq qgltltstqq prgppalqlr aqqpparqlp tsdqqakqqa
     2401 eavkqaqlff aqskptapep avedglvvnd ssddkavppk eaaee
//
LOCUS       XP_077505103            2419 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114939 isoform X10 [Amblyomma
            americanum].
ACCESSION   XP_077505103
VERSION     XP_077505103.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648977.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2419
                     /product="uncharacterized protein LOC144114939 isoform
                     X10"
                     /calculated_mol_wt=257603
     Region          2..237
                     /region_name="BAT2_N"
                     /note="BAT2 N-terminus; pfam07001"
                     /db_xref="CDD:462063"
     Region          <998..>1355
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          1265..>1499
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          <2110..2377
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..2419
                     /gene="LOC144114939"
                     /coded_by="XM_077648977.1:231..7490"
                     /db_xref="GeneID:144114939"
ORIGIN      
        1 mstvpglpsk gekgkpkyla ldinniykgk slenpkasaa pkhglqslgk vgagrrmppp
       61 vnlpslksen sgnnpnislv psggqgwgsa gkekgkspee nssgpqqqsq hsqsptshsq
      121 gqpaskqpqq qqqpppppqq qqqqppppqq pqpqpppppl ppvatstsap ggpssggtvg
      181 vgvgssagvk twssvtssqe gggpernflg hqspffpqef pklaggdvpi dgtqrsatds
      241 qygpgpslrp qmegtwsrgt lqqqqqqqpq qapggsggpg ggggqgcgpp gglppssgsg
      301 vngqasftpp prgalpsgvp pgggppsapa qpsyrqqavp pfvqqvygrg fpgnyapsys
      361 gvpggtrppy aysenrvrgv rnapdedgyq rpaiisekdl kgfdeilqnd sqdgwaaakg
      421 didynaklvf sddedsgssn rgagskaaei vpasesgkpg ptkgsekgec rpqgpagpkl
      481 sssrnrepgw dapsgqqpaq qraareppqt payldrderg rkawgapgsa elqpqpapls
      541 qqaqqappaq aapvrqwpqa qqaapptaap praagapple ylarstppaa tappamaaag
      601 fptgrvgppi pqqpshhtgf ggvgpvapsr gasrpgldql tgdeeelwrq rrqqqndema
      661 vtvertrqrr eeeekryeqi kqssaataaa ataanqqqqq pslpeesapv paekedldqr
      721 schssesree arggallpnr drpdfrqqss ggyqqnyqrp fknmpprflk kaemqrqqql
      781 rqqqqqqqqq qqqpqqpqqq qpqqqqqlqp qqqqpqqqpq qqqqqqpqqq pqasgglppp
      841 qgapsslsgm ggvsydprwv amgaggpylg qlgmvkplgs qrppesegst pyptaedrpy
      901 earearplec wppgagrpap pqadrpqqpl appmapphvp pggpydnwrp svapvyyggq
      961 vadfgrtpas ydqrlefppk rpdkeetpfd srsrgdsfrk erefedkqqr ekrenrdvmg
     1021 twsnareydq rlardkwere tqpsrpdsrd srasrdslrd eramppaeqr qdapmqqiep
     1081 hrlapervke kpqvpdwsdt pysaageskr rdwhthhppp vtqqqldsag pskknfvalr
     1141 rggvakdegk edskegkved hsrtdeeksk evakkddsek raatsrsrgg sggrfeanrg
     1201 rggrggngfg gvgyrgrsre yrrsrtergp rfdrkkdegs eessgveeek assrlrpkee
     1261 esegsdeacl dadrksrpgk esgrareprr deaknkeeks afsprgepsr rgrggavslg
     1321 rsargrypag gygpptskap fgkpeegskd eeankevsqg skqrsnssrp vvrrpnrmep
     1381 lpprfqknqs rrqdnpdrnk grggrgagpn kdalsdhgne dwetasessd vadrrgpeeg
     1441 vrqqpsgskk npssvpkasg gqerrrpgge arrgtggppg drngrgsvqr rdsvgggppa
     1501 rmgrgsgsrp ggrarqqrpg evtakgepsp aalvyrmdev klqdpggvqa altdlsarkh
     1561 tkkpeqgqqs grldkekpna lqgidisnya svvviddhpa dpslcdgddg gfqevtskkr
     1621 nricadgdsk kkkearskan vhgrqnklpp rlikkresdr misskaesql pakseagatd
     1681 smevastkah qhstfstkel gatgvirpiv nawdkpitha lrggsppssa pqpvttpavs
     1741 tadvlvssks ssfdrssdqh dsgidvsdqp asaassqrss psndgkppll gtsskglpam
     1801 pvgvpasagg edsgdmkldf tfdselaqfg dekqakhmai arslsseits anspispstd
     1861 elnlkiasvk kvwenvgplp tvlehgavsv sgddtgsaav qapsfgsfgg sgssdnsfat
     1921 sstateamaq qgqaeptqvt settfgsvsa lkaapsslsh hvasvvgtgh qhpsasnqhh
     1981 hhhhhqqqqq qpvqqqqqla maahhhsqaa qahgslqahq qqqammrqph qahsmleqqn
     2041 lskvralhal spppsaaass lagldvrama nlqvagmpqa vasppaallf tstqqmtqsa
     2101 alyqtllqdp vsrpvahqfs qathgypapp plgsyqgfnq ggmfvppppt hsgpdmftat
     2161 gaalrlqpty qagppvptsq vlgqqslppp aqqllssgra apggaplgts yypsqggyyp
     2221 sqqqgqalaf gsqptmhrtf hpppfkslel apdfgttppp kaqaakafpp gspfafgqgr
     2281 qaapptaagt gtppaggamr ppmhvlpmpp nmirsapppp saptkypgpi qrpqqgltlt
     2341 stqqprgppa lqlraqqppa rqlptsdqqa kqqaeavkqa qlffaqskpt apepavedgl
     2401 vvndssddka vppkeaaee
//
LOCUS       XP_077505104             634 aa            linear   INV 25-NOV-2025
DEFINITION  NDT-like domain-containing protein raw isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505104
VERSION     XP_077505104.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648978.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..634
                     /product="NDT-like domain-containing protein raw isoform
                     X1"
                     /calculated_mol_wt=70912
     Region          198..282
                     /region_name="Nuc_deoxyri_tr2"
                     /note="Nucleoside 2-deoxyribosyltransferase like;
                     pfam15891"
                     /db_xref="CDD:464926"
     Region          494..579
                     /region_name="Nuc_deoxyrib_tr"
                     /note="Nucleoside 2-deoxyribosyltransferase; cl46294"
                     /db_xref="CDD:480634"
     CDS             1..634
                     /gene="raw"
                     /coded_by="XM_077648978.1:16..1920"
                     /db_xref="GeneID:144114943"
ORIGIN      
        1 maditaggar erhsefplga fprlcaaarp sgratralrr lpsertcltl partaprdap
       61 aagssrqfld vllvsvlkma resfctmtai pvglfssalf sthaapppfy lcafcklich
      121 gsglkaesck ylckvhemwq cshthnnsft vgsfcffsse mkyahekrlp rsqplskpak
      181 dvrkllmkrr sesvessydv flggscnptt wrkdvaipkl ksygisyynp qvtqwipeli
      241 elenqakena kvmmfvidnq trsvasmies ahiagtrrkl ililneqspa gslvlgepis
      301 ekeyedllqg rnylrdlvem rgipvfqgis dalevtnrcl kedlwpqdis chknlvqvph
      361 lplgdkylkv qeafesfcsd dgkmhirdlk mafkvltgkd lpqeclevlr cnqqlamtes
      421 vgegdmddip itfdqfclil sefkhqtkng swlseflasf qrlytqlydr ivvprsalss
      481 rvteatlpay wshvylggst kdsswrddia ipilkkhglt hsvpfhspwn arlsplemhi
      541 insaqvllfv itktarcvae mlvavhyvgl gcnvilciqy lesdvvidge klselavkdy
      601 nrgrmylsdl atragvpvfs diseavlcaa qrch
//
LOCUS       XP_077505105             633 aa            linear   INV 25-NOV-2025
DEFINITION  NDT-like domain-containing protein raw isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505105
VERSION     XP_077505105.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648979.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..633
                     /product="NDT-like domain-containing protein raw isoform
                     X2"
                     /calculated_mol_wt=70948
     Region          197..281
                     /region_name="Nuc_deoxyri_tr2"
                     /note="Nucleoside 2-deoxyribosyltransferase like;
                     pfam15891"
                     /db_xref="CDD:464926"
     Region          493..578
                     /region_name="Nuc_deoxyrib_tr"
                     /note="Nucleoside 2-deoxyribosyltransferase; cl46294"
                     /db_xref="CDD:480634"
     CDS             1..633
                     /gene="raw"
                     /coded_by="XM_077648979.1:16..1917"
                     /db_xref="GeneID:144114943"
ORIGIN      
        1 maditaggar erhsefplga fprlcaaarp sgratralrr lpsertcltl partaprdap
       61 aagssrqfld vllvsvlkma resfctmtai pvglfsskav eawskfsgka gsltqqdlfp
      121 lfhtleiypt esqvhemwqc shthnnsftv gsfcffssem kyahekrlpr sqplskpakd
      181 vrkllmkrrs esvessydvf lggscnpttw rkdvaipklk sygisyynpq vtqwipelie
      241 lenqakenak vmmfvidnqt rsvasmiesa hiagtrrkli lilneqspag slvlgepise
      301 keyedllqgr nylrdlvemr gipvfqgisd alevtnrclk edlwpqdisc hknlvqvphl
      361 plgdkylkvq eafesfcsdd gkmhirdlkm afkvltgkdl pqeclevlrc nqqlamtesv
      421 gegdmddipi tfdqfclils efkhqtkngs wlseflasfq rlytqlydri vvprsalssr
      481 vteatlpayw shvylggstk dsswrddiai pilkkhglth svpfhspwna rlsplemhii
      541 nsaqvllfvi tktarcvaem lvavhyvglg cnvilciqyl esdvvidgek lselavkdyn
      601 rgrmylsdla tragvpvfsd iseavlcaaq rch
//
LOCUS       XP_077505106             633 aa            linear   INV 25-NOV-2025
DEFINITION  NDT-like domain-containing protein raw isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505106
VERSION     XP_077505106.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648980.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..633
                     /product="NDT-like domain-containing protein raw isoform
                     X3"
                     /calculated_mol_wt=70784
     Region          198..281
                     /region_name="Nuc_deoxyri_tr2"
                     /note="Nucleoside 2-deoxyribosyltransferase like;
                     pfam15891"
                     /db_xref="CDD:464926"
     Region          493..578
                     /region_name="Nuc_deoxyrib_tr"
                     /note="Nucleoside 2-deoxyribosyltransferase; cl46294"
                     /db_xref="CDD:480634"
     CDS             1..633
                     /gene="raw"
                     /coded_by="XM_077648980.1:16..1917"
                     /db_xref="GeneID:144114943"
ORIGIN      
        1 maditaggar erhsefplga fprlcaaarp sgratralrr lpsertcltl partaprdap
       61 aagssrqfld vllvsvlkma resfctmtai pvglfssalf sthaapppfy lcafcklich
      121 gsglkaesck ylckvhemwq cshthnnsft vgsfcffsse mkyahekrlp rsqplskpak
      181 dvrkllmkrr sesvessydv flggscnptt wrkdvaipkl ksygisyynp vtqwipelie
      241 lenqakenak vmmfvidnqt rsvasmiesa hiagtrrkli lilneqspag slvlgepise
      301 keyedllqgr nylrdlvemr gipvfqgisd alevtnrclk edlwpqdisc hknlvqvphl
      361 plgdkylkvq eafesfcsdd gkmhirdlkm afkvltgkdl pqeclevlrc nqqlamtesv
      421 gegdmddipi tfdqfclils efkhqtkngs wlseflasfq rlytqlydri vvprsalssr
      481 vteatlpayw shvylggstk dsswrddiai pilkkhglth svpfhspwna rlsplemhii
      541 nsaqvllfvi tktarcvaem lvavhyvglg cnvilciqyl esdvvidgek lselavkdyn
      601 rgrmylsdla tragvpvfsd iseavlcaaq rch
//
LOCUS       XP_077505107             586 aa            linear   INV 25-NOV-2025
DEFINITION  NDT-like domain-containing protein raw isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505107
VERSION     XP_077505107.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648981.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..586
                     /product="NDT-like domain-containing protein raw isoform
                     X4"
                     /calculated_mol_wt=65969
     Region          150..234
                     /region_name="Nuc_deoxyri_tr2"
                     /note="Nucleoside 2-deoxyribosyltransferase like;
                     pfam15891"
                     /db_xref="CDD:464926"
     Region          446..531
                     /region_name="Nuc_deoxyrib_tr"
                     /note="Nucleoside 2-deoxyribosyltransferase; cl46294"
                     /db_xref="CDD:480634"
     CDS             1..586
                     /gene="raw"
                     /coded_by="XM_077648981.1:188..1948"
                     /db_xref="GeneID:144114943"
ORIGIN      
        1 msssarvslr riqtcglqyf ldvllvsvlk maresfctmt aipvglfssa lfsthaappp
       61 fylcafckli chgsglkaes ckylckvhem wqcshthnns ftvgsfcffs semkyahekr
      121 lprsqplskp akdvrkllmk rrsesvessy dvflggscnp ttwrkdvaip klksygisyy
      181 npqvtqwipe lielenqake nakvmmfvid nqtrsvasmi esahiagtrr klililneqs
      241 pagslvlgep isekeyedll qgrnylrdlv emrgipvfqg isdalevtnr clkedlwpqd
      301 ischknlvqv phlplgdkyl kvqeafesfc sddgkmhird lkmafkvltg kdlpqeclev
      361 lrcnqqlamt esvgegdmdd ipitfdqfcl ilsefkhqtk ngswlsefla sfqrlytqly
      421 drivvprsal ssrvteatlp aywshvylgg stkdsswrdd iaipilkkhg lthsvpfhsp
      481 wnarlsplem hiinsaqvll fvitktarcv aemlvavhyv glgcnvilci qylesdvvid
      541 geklselavk dynrgrmyls dlatragvpv fsdiseavlc aaqrch
//
LOCUS       XP_077505108             585 aa            linear   INV 25-NOV-2025
DEFINITION  NDT-like domain-containing protein raw isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077505108
VERSION     XP_077505108.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648982.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..585
                     /product="NDT-like domain-containing protein raw isoform
                     X5"
                     /calculated_mol_wt=66004
     Region          149..233
                     /region_name="Nuc_deoxyri_tr2"
                     /note="Nucleoside 2-deoxyribosyltransferase like;
                     pfam15891"
                     /db_xref="CDD:464926"
     Region          445..530
                     /region_name="Nuc_deoxyrib_tr"
                     /note="Nucleoside 2-deoxyribosyltransferase; cl46294"
                     /db_xref="CDD:480634"
     CDS             1..585
                     /gene="raw"
                     /coded_by="XM_077648982.1:179..1936"
                     /db_xref="GeneID:144114943"
ORIGIN      
        1 msssarvslr riqtcglqyf ldvllvsvlk maresfctmt aipvglfssk aveawskfsg
       61 kagsltqqdl fplfhtleiy ptesqvhemw qcshthnnsf tvgsfcffss emkyahekrl
      121 prsqplskpa kdvrkllmkr rsesvessyd vflggscnpt twrkdvaipk lksygisyyn
      181 pqvtqwipel ielenqaken akvmmfvidn qtrsvasmie sahiagtrrk lililneqsp
      241 agslvlgepi sekeyedllq grnylrdlve mrgipvfqgi sdalevtnrc lkedlwpqdi
      301 schknlvqvp hlplgdkylk vqeafesfcs ddgkmhirdl kmafkvltgk dlpqeclevl
      361 rcnqqlamte svgegdmddi pitfdqfcli lsefkhqtkn gswlseflas fqrlytqlyd
      421 rivvprsals srvteatlpa ywshvylggs tkdsswrddi aipilkkhgl thsvpfhspw
      481 narlsplemh iinsaqvllf vitktarcva emlvavhyvg lgcnvilciq ylesdvvidg
      541 eklselavkd ynrgrmylsd latragvpvf sdiseavlca aqrch
//
LOCUS       XP_077505109             578 aa            linear   INV 25-NOV-2025
DEFINITION  NDT-like domain-containing protein raw isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077505109
VERSION     XP_077505109.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648983.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..578
                     /product="NDT-like domain-containing protein raw isoform
                     X6"
                     /calculated_mol_wt=65170
     Region          142..226
                     /region_name="Nuc_deoxyri_tr2"
                     /note="Nucleoside 2-deoxyribosyltransferase like;
                     pfam15891"
                     /db_xref="CDD:464926"
     Region          438..523
                     /region_name="Nuc_deoxyrib_tr"
                     /note="Nucleoside 2-deoxyribosyltransferase; cl46294"
                     /db_xref="CDD:480634"
     CDS             1..578
                     /gene="raw"
                     /coded_by="XM_077648983.1:16..1752"
                     /db_xref="GeneID:144114943"
ORIGIN      
        1 mpafkllrll rfldvllvsv lkmaresfct mtaipvglfs salfsthaap ppfylcafck
       61 lichgsglka esckylckvh emwqcshthn nsftvgsfcf fssemkyahe krlprsqpls
      121 kpakdvrkll mkrrsesves sydvflggsc npttwrkdva ipklksygis yynpqvtqwi
      181 pelielenqa kenakvmmfv idnqtrsvas miesahiagt rrklililne qspagslvlg
      241 episekeyed llqgrnylrd lvemrgipvf qgisdalevt nrclkedlwp qdischknlv
      301 qvphlplgdk ylkvqeafes fcsddgkmhi rdlkmafkvl tgkdlpqecl evlrcnqqla
      361 mtesvgegdm ddipitfdqf clilsefkhq tkngswlsef lasfqrlytq lydrivvprs
      421 alssrvteat lpaywshvyl ggstkdsswr ddiaipilkk hglthsvpfh spwnarlspl
      481 emhiinsaqv llfvitktar cvaemlvavh yvglgcnvil ciqylesdvv idgeklsela
      541 vkdynrgrmy lsdlatragv pvfsdiseav lcaaqrch
//
LOCUS       XP_077505110             573 aa            linear   INV 25-NOV-2025
DEFINITION  NDT-like domain-containing protein raw isoform X7 [Amblyomma
            americanum].
ACCESSION   XP_077505110
VERSION     XP_077505110.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648984.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..573
                     /product="NDT-like domain-containing protein raw isoform
                     X7"
                     /calculated_mol_wt=64420
     Region          137..221
                     /region_name="Nuc_deoxyri_tr2"
                     /note="Nucleoside 2-deoxyribosyltransferase like;
                     pfam15891"
                     /db_xref="CDD:464926"
     Region          433..518
                     /region_name="Nuc_deoxyrib_tr"
                     /note="Nucleoside 2-deoxyribosyltransferase; cl46294"
                     /db_xref="CDD:480634"
     CDS             1..573
                     /gene="raw"
                     /coded_by="XM_077648984.1:145..1866"
                     /db_xref="GeneID:144114943"
ORIGIN      
        1 maamgqfldv llvsvlkmar esfctmtaip vglfssalfs thaapppfyl cafcklichg
       61 sglkaescky lckvhemwqc shthnnsftv gsfcffssem kyahekrlpr sqplskpakd
      121 vrkllmkrrs esvessydvf lggscnpttw rkdvaipklk sygisyynpq vtqwipelie
      181 lenqakenak vmmfvidnqt rsvasmiesa hiagtrrkli lilneqspag slvlgepise
      241 keyedllqgr nylrdlvemr gipvfqgisd alevtnrclk edlwpqdisc hknlvqvphl
      301 plgdkylkvq eafesfcsdd gkmhirdlkm afkvltgkdl pqeclevlrc nqqlamtesv
      361 gegdmddipi tfdqfclils efkhqtkngs wlseflasfq rlytqlydri vvprsalssr
      421 vteatlpayw shvylggstk dsswrddiai pilkkhglth svpfhspwna rlsplemhii
      481 nsaqvllfvi tktarcvaem lvavhyvglg cnvilciqyl esdvvidgek lselavkdyn
      541 rgrmylsdla tragvpvfsd iseavlcaaq rch
//
LOCUS       XP_077505111             572 aa            linear   INV 25-NOV-2025
DEFINITION  NDT-like domain-containing protein raw isoform X8 [Amblyomma
            americanum].
ACCESSION   XP_077505111
VERSION     XP_077505111.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648985.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..572
                     /product="NDT-like domain-containing protein raw isoform
                     X8"
                     /calculated_mol_wt=64456
     Region          136..220
                     /region_name="Nuc_deoxyri_tr2"
                     /note="Nucleoside 2-deoxyribosyltransferase like;
                     pfam15891"
                     /db_xref="CDD:464926"
     Region          432..517
                     /region_name="Nuc_deoxyrib_tr"
                     /note="Nucleoside 2-deoxyribosyltransferase; cl46294"
                     /db_xref="CDD:480634"
     CDS             1..572
                     /gene="raw"
                     /coded_by="XM_077648985.1:189..1907"
                     /db_xref="GeneID:144114943"
ORIGIN      
        1 maamgqfldv llvsvlkmar esfctmtaip vglfsskave awskfsgkag sltqqdlfpl
       61 fhtleiypte sqvhemwqcs hthnnsftvg sfcffssemk yahekrlprs qplskpakdv
      121 rkllmkrrse svessydvfl ggscnpttwr kdvaipklks ygisyynpqv tqwipeliel
      181 enqakenakv mmfvidnqtr svasmiesah iagtrrklil ilneqspags lvlgepisek
      241 eyedllqgrn ylrdlvemrg ipvfqgisda levtnrclke dlwpqdisch knlvqvphlp
      301 lgdkylkvqe afesfcsddg kmhirdlkma fkvltgkdlp qeclevlrcn qqlamtesvg
      361 egdmddipit fdqfclilse fkhqtkngsw lseflasfqr lytqlydriv vprsalssrv
      421 teatlpayws hvylggstkd sswrddiaip ilkkhglths vpfhspwnar lsplemhiin
      481 saqvllfvit ktarcvaeml vavhyvglgc nvilciqyle sdvvidgekl selavkdynr
      541 grmylsdlat ragvpvfsdi seavlcaaqr ch
//
LOCUS       XP_077505112             556 aa            linear   INV 25-NOV-2025
DEFINITION  NDT-like domain-containing protein raw isoform X9 [Amblyomma
            americanum].
ACCESSION   XP_077505112
VERSION     XP_077505112.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648986.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..556
                     /product="NDT-like domain-containing protein raw isoform
                     X9"
                     /calculated_mol_wt=62603
     Region          120..204
                     /region_name="Nuc_deoxyri_tr2"
                     /note="Nucleoside 2-deoxyribosyltransferase like;
                     pfam15891"
                     /db_xref="CDD:464926"
     Region          416..501
                     /region_name="Nuc_deoxyrib_tr"
                     /note="Nucleoside 2-deoxyribosyltransferase; cl46294"
                     /db_xref="CDD:480634"
     CDS             1..556
                     /gene="raw"
                     /coded_by="XM_077648986.1:284..1954"
                     /db_xref="GeneID:144114943"
ORIGIN      
        1 maresfctmt aipvglfssa lfsthaappp fylcafckli chgsglkaes ckylckvhem
       61 wqcshthnns ftvgsfcffs semkyahekr lprsqplskp akdvrkllmk rrsesvessy
      121 dvflggscnp ttwrkdvaip klksygisyy npqvtqwipe lielenqake nakvmmfvid
      181 nqtrsvasmi esahiagtrr klililneqs pagslvlgep isekeyedll qgrnylrdlv
      241 emrgipvfqg isdalevtnr clkedlwpqd ischknlvqv phlplgdkyl kvqeafesfc
      301 sddgkmhird lkmafkvltg kdlpqeclev lrcnqqlamt esvgegdmdd ipitfdqfcl
      361 ilsefkhqtk ngswlsefla sfqrlytqly drivvprsal ssrvteatlp aywshvylgg
      421 stkdsswrdd iaipilkkhg lthsvpfhsp wnarlsplem hiinsaqvll fvitktarcv
      481 aemlvavhyv glgcnvilci qylesdvvid geklselavk dynrgrmyls dlatragvpv
      541 fsdiseavlc aaqrch
//
LOCUS       XP_077505113             556 aa            linear   INV 25-NOV-2025
DEFINITION  NDT-like domain-containing protein raw isoform X9 [Amblyomma
            americanum].
ACCESSION   XP_077505113
VERSION     XP_077505113.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648987.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..556
                     /product="NDT-like domain-containing protein raw isoform
                     X9"
                     /calculated_mol_wt=62603
     Region          120..204
                     /region_name="Nuc_deoxyri_tr2"
                     /note="Nucleoside 2-deoxyribosyltransferase like;
                     pfam15891"
                     /db_xref="CDD:464926"
     Region          416..501
                     /region_name="Nuc_deoxyrib_tr"
                     /note="Nucleoside 2-deoxyribosyltransferase; cl46294"
                     /db_xref="CDD:480634"
     CDS             1..556
                     /gene="raw"
                     /coded_by="XM_077648987.1:1024..2694"
                     /db_xref="GeneID:144114943"
ORIGIN      
        1 maresfctmt aipvglfssa lfsthaappp fylcafckli chgsglkaes ckylckvhem
       61 wqcshthnns ftvgsfcffs semkyahekr lprsqplskp akdvrkllmk rrsesvessy
      121 dvflggscnp ttwrkdvaip klksygisyy npqvtqwipe lielenqake nakvmmfvid
      181 nqtrsvasmi esahiagtrr klililneqs pagslvlgep isekeyedll qgrnylrdlv
      241 emrgipvfqg isdalevtnr clkedlwpqd ischknlvqv phlplgdkyl kvqeafesfc
      301 sddgkmhird lkmafkvltg kdlpqeclev lrcnqqlamt esvgegdmdd ipitfdqfcl
      361 ilsefkhqtk ngswlsefla sfqrlytqly drivvprsal ssrvteatlp aywshvylgg
      421 stkdsswrdd iaipilkkhg lthsvpfhsp wnarlsplem hiinsaqvll fvitktarcv
      481 aemlvavhyv glgcnvilci qylesdvvid geklselavk dynrgrmyls dlatragvpv
      541 fsdiseavlc aaqrch
//
LOCUS       XP_077505114             556 aa            linear   INV 25-NOV-2025
DEFINITION  NDT-like domain-containing protein raw isoform X9 [Amblyomma
            americanum].
ACCESSION   XP_077505114
VERSION     XP_077505114.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648988.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..556
                     /product="NDT-like domain-containing protein raw isoform
                     X9"
                     /calculated_mol_wt=62603
     Region          120..204
                     /region_name="Nuc_deoxyri_tr2"
                     /note="Nucleoside 2-deoxyribosyltransferase like;
                     pfam15891"
                     /db_xref="CDD:464926"
     Region          416..501
                     /region_name="Nuc_deoxyrib_tr"
                     /note="Nucleoside 2-deoxyribosyltransferase; cl46294"
                     /db_xref="CDD:480634"
     CDS             1..556
                     /gene="raw"
                     /coded_by="XM_077648988.1:180..1850"
                     /db_xref="GeneID:144114943"
ORIGIN      
        1 maresfctmt aipvglfssa lfsthaappp fylcafckli chgsglkaes ckylckvhem
       61 wqcshthnns ftvgsfcffs semkyahekr lprsqplskp akdvrkllmk rrsesvessy
      121 dvflggscnp ttwrkdvaip klksygisyy npqvtqwipe lielenqake nakvmmfvid
      181 nqtrsvasmi esahiagtrr klililneqs pagslvlgep isekeyedll qgrnylrdlv
      241 emrgipvfqg isdalevtnr clkedlwpqd ischknlvqv phlplgdkyl kvqeafesfc
      301 sddgkmhird lkmafkvltg kdlpqeclev lrcnqqlamt esvgegdmdd ipitfdqfcl
      361 ilsefkhqtk ngswlsefla sfqrlytqly drivvprsal ssrvteatlp aywshvylgg
      421 stkdsswrdd iaipilkkhg lthsvpfhsp wnarlsplem hiinsaqvll fvitktarcv
      481 aemlvavhyv glgcnvilci qylesdvvid geklselavk dynrgrmyls dlatragvpv
      541 fsdiseavlc aaqrch
//
LOCUS       XP_077505115             555 aa            linear   INV 25-NOV-2025
DEFINITION  NDT-like domain-containing protein raw isoform X10 [Amblyomma
            americanum].
ACCESSION   XP_077505115
VERSION     XP_077505115.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648989.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..555
                     /product="NDT-like domain-containing protein raw isoform
                     X10"
                     /calculated_mol_wt=62638
     Region          119..203
                     /region_name="Nuc_deoxyri_tr2"
                     /note="Nucleoside 2-deoxyribosyltransferase like;
                     pfam15891"
                     /db_xref="CDD:464926"
     Region          415..500
                     /region_name="Nuc_deoxyrib_tr"
                     /note="Nucleoside 2-deoxyribosyltransferase; cl46294"
                     /db_xref="CDD:480634"
     CDS             1..555
                     /gene="raw"
                     /coded_by="XM_077648989.1:365..2032"
                     /db_xref="GeneID:144114943"
ORIGIN      
        1 maresfctmt aipvglfssk aveawskfsg kagsltqqdl fplfhtleiy ptesqvhemw
       61 qcshthnnsf tvgsfcffss emkyahekrl prsqplskpa kdvrkllmkr rsesvessyd
      121 vflggscnpt twrkdvaipk lksygisyyn pqvtqwipel ielenqaken akvmmfvidn
      181 qtrsvasmie sahiagtrrk lililneqsp agslvlgepi sekeyedllq grnylrdlve
      241 mrgipvfqgi sdalevtnrc lkedlwpqdi schknlvqvp hlplgdkylk vqeafesfcs
      301 ddgkmhirdl kmafkvltgk dlpqeclevl rcnqqlamte svgegdmddi pitfdqfcli
      361 lsefkhqtkn gswlseflas fqrlytqlyd rivvprsals srvteatlpa ywshvylggs
      421 tkdsswrddi aipilkkhgl thsvpfhspw narlsplemh iinsaqvllf vitktarcva
      481 emlvavhyvg lgcnvilciq ylesdvvidg eklselavkd ynrgrmylsd latragvpvf
      541 sdiseavlca aqrch
//
LOCUS       XP_077505116             555 aa            linear   INV 25-NOV-2025
DEFINITION  NDT-like domain-containing protein raw isoform X10 [Amblyomma
            americanum].
ACCESSION   XP_077505116
VERSION     XP_077505116.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648990.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..555
                     /product="NDT-like domain-containing protein raw isoform
                     X10"
                     /calculated_mol_wt=62638
     Region          119..203
                     /region_name="Nuc_deoxyri_tr2"
                     /note="Nucleoside 2-deoxyribosyltransferase like;
                     pfam15891"
                     /db_xref="CDD:464926"
     Region          415..500
                     /region_name="Nuc_deoxyrib_tr"
                     /note="Nucleoside 2-deoxyribosyltransferase; cl46294"
                     /db_xref="CDD:480634"
     CDS             1..555
                     /gene="raw"
                     /coded_by="XM_077648990.1:1025..2692"
                     /db_xref="GeneID:144114943"
ORIGIN      
        1 maresfctmt aipvglfssk aveawskfsg kagsltqqdl fplfhtleiy ptesqvhemw
       61 qcshthnnsf tvgsfcffss emkyahekrl prsqplskpa kdvrkllmkr rsesvessyd
      121 vflggscnpt twrkdvaipk lksygisyyn pqvtqwipel ielenqaken akvmmfvidn
      181 qtrsvasmie sahiagtrrk lililneqsp agslvlgepi sekeyedllq grnylrdlve
      241 mrgipvfqgi sdalevtnrc lkedlwpqdi schknlvqvp hlplgdkylk vqeafesfcs
      301 ddgkmhirdl kmafkvltgk dlpqeclevl rcnqqlamte svgegdmddi pitfdqfcli
      361 lsefkhqtkn gswlseflas fqrlytqlyd rivvprsals srvteatlpa ywshvylggs
      421 tkdsswrddi aipilkkhgl thsvpfhspw narlsplemh iinsaqvllf vitktarcva
      481 emlvavhyvg lgcnvilciq ylesdvvidg eklselavkd ynrgrmylsd latragvpvf
      541 sdiseavlca aqrch
//
LOCUS       XP_077505117             555 aa            linear   INV 25-NOV-2025
DEFINITION  NDT-like domain-containing protein raw isoform X10 [Amblyomma
            americanum].
ACCESSION   XP_077505117
VERSION     XP_077505117.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648991.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..555
                     /product="NDT-like domain-containing protein raw isoform
                     X10"
                     /calculated_mol_wt=62638
     Region          119..203
                     /region_name="Nuc_deoxyri_tr2"
                     /note="Nucleoside 2-deoxyribosyltransferase like;
                     pfam15891"
                     /db_xref="CDD:464926"
     Region          415..500
                     /region_name="Nuc_deoxyrib_tr"
                     /note="Nucleoside 2-deoxyribosyltransferase; cl46294"
                     /db_xref="CDD:480634"
     CDS             1..555
                     /gene="raw"
                     /coded_by="XM_077648991.1:174..1841"
                     /db_xref="GeneID:144114943"
ORIGIN      
        1 maresfctmt aipvglfssk aveawskfsg kagsltqqdl fplfhtleiy ptesqvhemw
       61 qcshthnnsf tvgsfcffss emkyahekrl prsqplskpa kdvrkllmkr rsesvessyd
      121 vflggscnpt twrkdvaipk lksygisyyn pqvtqwipel ielenqaken akvmmfvidn
      181 qtrsvasmie sahiagtrrk lililneqsp agslvlgepi sekeyedllq grnylrdlve
      241 mrgipvfqgi sdalevtnrc lkedlwpqdi schknlvqvp hlplgdkylk vqeafesfcs
      301 ddgkmhirdl kmafkvltgk dlpqeclevl rcnqqlamte svgegdmddi pitfdqfcli
      361 lsefkhqtkn gswlseflas fqrlytqlyd rivvprsals srvteatlpa ywshvylggs
      421 tkdsswrddi aipilkkhgl thsvpfhspw narlsplemh iinsaqvllf vitktarcva
      481 emlvavhyvg lgcnvilciq ylesdvvidg eklselavkd ynrgrmylsd latragvpvf
      541 sdiseavlca aqrch
//
LOCUS       XP_077505118             497 aa            linear   INV 25-NOV-2025
DEFINITION  NDT-like domain-containing protein raw isoform X11 [Amblyomma
            americanum].
ACCESSION   XP_077505118
VERSION     XP_077505118.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648992.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..497
                     /product="NDT-like domain-containing protein raw isoform
                     X11"
                     /calculated_mol_wt=56205
     Region          61..145
                     /region_name="Nuc_deoxyri_tr2"
                     /note="Nucleoside 2-deoxyribosyltransferase like;
                     pfam15891"
                     /db_xref="CDD:464926"
     Region          357..442
                     /region_name="Nuc_deoxyrib_tr"
                     /note="Nucleoside 2-deoxyribosyltransferase; cl46294"
                     /db_xref="CDD:480634"
     CDS             1..497
                     /gene="raw"
                     /coded_by="XM_077648992.1:555..2048"
                     /db_xref="GeneID:144114943"
ORIGIN      
        1 mwqcshthnn sftvgsfcff ssemkyahek rlprsqplsk pakdvrkllm krrsesvess
       61 ydvflggscn pttwrkdvai pklksygisy ynpqvtqwip elielenqak enakvmmfvi
      121 dnqtrsvasm iesahiagtr rklililneq spagslvlge pisekeyedl lqgrnylrdl
      181 vemrgipvfq gisdalevtn rclkedlwpq dischknlvq vphlplgdky lkvqeafesf
      241 csddgkmhir dlkmafkvlt gkdlpqecle vlrcnqqlam tesvgegdmd dipitfdqfc
      301 lilsefkhqt kngswlsefl asfqrlytql ydrivvprsa lssrvteatl paywshvylg
      361 gstkdsswrd diaipilkkh glthsvpfhs pwnarlsple mhiinsaqvl lfvitktarc
      421 vaemlvavhy vglgcnvilc iqylesdvvi dgeklselav kdynrgrmyl sdlatragvp
      481 vfsdiseavl caaqrch
//
LOCUS       XP_077505119             497 aa            linear   INV 25-NOV-2025
DEFINITION  NDT-like domain-containing protein raw isoform X11 [Amblyomma
            americanum].
ACCESSION   XP_077505119
VERSION     XP_077505119.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648993.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..497
                     /product="NDT-like domain-containing protein raw isoform
                     X11"
                     /calculated_mol_wt=56205
     Region          61..145
                     /region_name="Nuc_deoxyri_tr2"
                     /note="Nucleoside 2-deoxyribosyltransferase like;
                     pfam15891"
                     /db_xref="CDD:464926"
     Region          357..442
                     /region_name="Nuc_deoxyrib_tr"
                     /note="Nucleoside 2-deoxyribosyltransferase; cl46294"
                     /db_xref="CDD:480634"
     CDS             1..497
                     /gene="raw"
                     /coded_by="XM_077648993.1:551..2044"
                     /db_xref="GeneID:144114943"
ORIGIN      
        1 mwqcshthnn sftvgsfcff ssemkyahek rlprsqplsk pakdvrkllm krrsesvess
       61 ydvflggscn pttwrkdvai pklksygisy ynpqvtqwip elielenqak enakvmmfvi
      121 dnqtrsvasm iesahiagtr rklililneq spagslvlge pisekeyedl lqgrnylrdl
      181 vemrgipvfq gisdalevtn rclkedlwpq dischknlvq vphlplgdky lkvqeafesf
      241 csddgkmhir dlkmafkvlt gkdlpqecle vlrcnqqlam tesvgegdmd dipitfdqfc
      301 lilsefkhqt kngswlsefl asfqrlytql ydrivvprsa lssrvteatl paywshvylg
      361 gstkdsswrd diaipilkkh glthsvpfhs pwnarlsple mhiinsaqvl lfvitktarc
      421 vaemlvavhy vglgcnvilc iqylesdvvi dgeklselav kdynrgrmyl sdlatragvp
      481 vfsdiseavl caaqrch
//
LOCUS       XP_077505120             497 aa            linear   INV 25-NOV-2025
DEFINITION  NDT-like domain-containing protein raw isoform X11 [Amblyomma
            americanum].
ACCESSION   XP_077505120
VERSION     XP_077505120.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648994.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..497
                     /product="NDT-like domain-containing protein raw isoform
                     X11"
                     /calculated_mol_wt=56205
     Region          61..145
                     /region_name="Nuc_deoxyri_tr2"
                     /note="Nucleoside 2-deoxyribosyltransferase like;
                     pfam15891"
                     /db_xref="CDD:464926"
     Region          357..442
                     /region_name="Nuc_deoxyrib_tr"
                     /note="Nucleoside 2-deoxyribosyltransferase; cl46294"
                     /db_xref="CDD:480634"
     CDS             1..497
                     /gene="raw"
                     /coded_by="XM_077648994.1:564..2057"
                     /db_xref="GeneID:144114943"
ORIGIN      
        1 mwqcshthnn sftvgsfcff ssemkyahek rlprsqplsk pakdvrkllm krrsesvess
       61 ydvflggscn pttwrkdvai pklksygisy ynpqvtqwip elielenqak enakvmmfvi
      121 dnqtrsvasm iesahiagtr rklililneq spagslvlge pisekeyedl lqgrnylrdl
      181 vemrgipvfq gisdalevtn rclkedlwpq dischknlvq vphlplgdky lkvqeafesf
      241 csddgkmhir dlkmafkvlt gkdlpqecle vlrcnqqlam tesvgegdmd dipitfdqfc
      301 lilsefkhqt kngswlsefl asfqrlytql ydrivvprsa lssrvteatl paywshvylg
      361 gstkdsswrd diaipilkkh glthsvpfhs pwnarlsple mhiinsaqvl lfvitktarc
      421 vaemlvavhy vglgcnvilc iqylesdvvi dgeklselav kdynrgrmyl sdlatragvp
      481 vfsdiseavl caaqrch
//
LOCUS       XP_077505121             497 aa            linear   INV 25-NOV-2025
DEFINITION  NDT-like domain-containing protein raw isoform X11 [Amblyomma
            americanum].
ACCESSION   XP_077505121
VERSION     XP_077505121.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648995.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..497
                     /product="NDT-like domain-containing protein raw isoform
                     X11"
                     /calculated_mol_wt=56205
     Region          61..145
                     /region_name="Nuc_deoxyri_tr2"
                     /note="Nucleoside 2-deoxyribosyltransferase like;
                     pfam15891"
                     /db_xref="CDD:464926"
     Region          357..442
                     /region_name="Nuc_deoxyrib_tr"
                     /note="Nucleoside 2-deoxyribosyltransferase; cl46294"
                     /db_xref="CDD:480634"
     CDS             1..497
                     /gene="raw"
                     /coded_by="XM_077648995.1:1305..2798"
                     /db_xref="GeneID:144114943"
ORIGIN      
        1 mwqcshthnn sftvgsfcff ssemkyahek rlprsqplsk pakdvrkllm krrsesvess
       61 ydvflggscn pttwrkdvai pklksygisy ynpqvtqwip elielenqak enakvmmfvi
      121 dnqtrsvasm iesahiagtr rklililneq spagslvlge pisekeyedl lqgrnylrdl
      181 vemrgipvfq gisdalevtn rclkedlwpq dischknlvq vphlplgdky lkvqeafesf
      241 csddgkmhir dlkmafkvlt gkdlpqecle vlrcnqqlam tesvgegdmd dipitfdqfc
      301 lilsefkhqt kngswlsefl asfqrlytql ydrivvprsa lssrvteatl paywshvylg
      361 gstkdsswrd diaipilkkh glthsvpfhs pwnarlsple mhiinsaqvl lfvitktarc
      421 vaemlvavhy vglgcnvilc iqylesdvvi dgeklselav kdynrgrmyl sdlatragvp
      481 vfsdiseavl caaqrch
//
LOCUS       XP_077505122             497 aa            linear   INV 25-NOV-2025
DEFINITION  NDT-like domain-containing protein raw isoform X11 [Amblyomma
            americanum].
ACCESSION   XP_077505122
VERSION     XP_077505122.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648996.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..497
                     /product="NDT-like domain-containing protein raw isoform
                     X11"
                     /calculated_mol_wt=56205
     Region          61..145
                     /region_name="Nuc_deoxyri_tr2"
                     /note="Nucleoside 2-deoxyribosyltransferase like;
                     pfam15891"
                     /db_xref="CDD:464926"
     Region          357..442
                     /region_name="Nuc_deoxyrib_tr"
                     /note="Nucleoside 2-deoxyribosyltransferase; cl46294"
                     /db_xref="CDD:480634"
     CDS             1..497
                     /gene="raw"
                     /coded_by="XM_077648996.1:438..1931"
                     /db_xref="GeneID:144114943"
ORIGIN      
        1 mwqcshthnn sftvgsfcff ssemkyahek rlprsqplsk pakdvrkllm krrsesvess
       61 ydvflggscn pttwrkdvai pklksygisy ynpqvtqwip elielenqak enakvmmfvi
      121 dnqtrsvasm iesahiagtr rklililneq spagslvlge pisekeyedl lqgrnylrdl
      181 vemrgipvfq gisdalevtn rclkedlwpq dischknlvq vphlplgdky lkvqeafesf
      241 csddgkmhir dlkmafkvlt gkdlpqecle vlrcnqqlam tesvgegdmd dipitfdqfc
      301 lilsefkhqt kngswlsefl asfqrlytql ydrivvprsa lssrvteatl paywshvylg
      361 gstkdsswrd diaipilkkh glthsvpfhs pwnarlsple mhiinsaqvl lfvitktarc
      421 vaemlvavhy vglgcnvilc iqylesdvvi dgeklselav kdynrgrmyl sdlatragvp
      481 vfsdiseavl caaqrch
//
LOCUS       XP_077505123             497 aa            linear   INV 25-NOV-2025
DEFINITION  NDT-like domain-containing protein raw isoform X11 [Amblyomma
            americanum].
ACCESSION   XP_077505123
VERSION     XP_077505123.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648997.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..497
                     /product="NDT-like domain-containing protein raw isoform
                     X11"
                     /calculated_mol_wt=56205
     Region          61..145
                     /region_name="Nuc_deoxyri_tr2"
                     /note="Nucleoside 2-deoxyribosyltransferase like;
                     pfam15891"
                     /db_xref="CDD:464926"
     Region          357..442
                     /region_name="Nuc_deoxyrib_tr"
                     /note="Nucleoside 2-deoxyribosyltransferase; cl46294"
                     /db_xref="CDD:480634"
     CDS             1..497
                     /gene="raw"
                     /coded_by="XM_077648997.1:332..1825"
                     /db_xref="GeneID:144114943"
ORIGIN      
        1 mwqcshthnn sftvgsfcff ssemkyahek rlprsqplsk pakdvrkllm krrsesvess
       61 ydvflggscn pttwrkdvai pklksygisy ynpqvtqwip elielenqak enakvmmfvi
      121 dnqtrsvasm iesahiagtr rklililneq spagslvlge pisekeyedl lqgrnylrdl
      181 vemrgipvfq gisdalevtn rclkedlwpq dischknlvq vphlplgdky lkvqeafesf
      241 csddgkmhir dlkmafkvlt gkdlpqecle vlrcnqqlam tesvgegdmd dipitfdqfc
      301 lilsefkhqt kngswlsefl asfqrlytql ydrivvprsa lssrvteatl paywshvylg
      361 gstkdsswrd diaipilkkh glthsvpfhs pwnarlsple mhiinsaqvl lfvitktarc
      421 vaemlvavhy vglgcnvilc iqylesdvvi dgeklselav kdynrgrmyl sdlatragvp
      481 vfsdiseavl caaqrch
//
LOCUS       XP_077505124             497 aa            linear   INV 25-NOV-2025
DEFINITION  NDT-like domain-containing protein raw isoform X11 [Amblyomma
            americanum].
ACCESSION   XP_077505124
VERSION     XP_077505124.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648998.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..497
                     /product="NDT-like domain-containing protein raw isoform
                     X11"
                     /calculated_mol_wt=56205
     Region          61..145
                     /region_name="Nuc_deoxyri_tr2"
                     /note="Nucleoside 2-deoxyribosyltransferase like;
                     pfam15891"
                     /db_xref="CDD:464926"
     Region          357..442
                     /region_name="Nuc_deoxyrib_tr"
                     /note="Nucleoside 2-deoxyribosyltransferase; cl46294"
                     /db_xref="CDD:480634"
     CDS             1..497
                     /gene="raw"
                     /coded_by="XM_077648998.1:1086..2579"
                     /db_xref="GeneID:144114943"
ORIGIN      
        1 mwqcshthnn sftvgsfcff ssemkyahek rlprsqplsk pakdvrkllm krrsesvess
       61 ydvflggscn pttwrkdvai pklksygisy ynpqvtqwip elielenqak enakvmmfvi
      121 dnqtrsvasm iesahiagtr rklililneq spagslvlge pisekeyedl lqgrnylrdl
      181 vemrgipvfq gisdalevtn rclkedlwpq dischknlvq vphlplgdky lkvqeafesf
      241 csddgkmhir dlkmafkvlt gkdlpqecle vlrcnqqlam tesvgegdmd dipitfdqfc
      301 lilsefkhqt kngswlsefl asfqrlytql ydrivvprsa lssrvteatl paywshvylg
      361 gstkdsswrd diaipilkkh glthsvpfhs pwnarlsple mhiinsaqvl lfvitktarc
      421 vaemlvavhy vglgcnvilc iqylesdvvi dgeklselav kdynrgrmyl sdlatragvp
      481 vfsdiseavl caaqrch
//
LOCUS       XP_077505125             179 aa            linear   INV 25-NOV-2025
DEFINITION  sulfiredoxin-1-like [Amblyomma americanum].
ACCESSION   XP_077505125
VERSION     XP_077505125.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077648999.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..179
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..179
                     /product="sulfiredoxin-1-like"
                     /calculated_mol_wt=19582
     Region          81..172
                     /region_name="Srx"
                     /note="Sulfiredoxin reactivates peroxiredoxins after
                     oxidative inactivation; cd16395"
                     /db_xref="CDD:319253"
     Site            order(82..85,90..94,122,124..125,133..135,138..139,156,
                     159..160,165,168..169)
                     /site_type="other"
                     /note="peroxiredoxin binding site [polypeptide binding]"
                     /db_xref="CDD:319253"
     Site            order(101,104..105,108,117,119,139..143)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:319253"
     CDS             1..179
                     /gene="LOC144114945"
                     /coded_by="XM_077648999.1:155..694"
                     /db_xref="GeneID:144114945"
ORIGIN      
        1 mfmpsgavat splraltcgg llrllllggq aashswenhr ahctsrgsdd ddrrhcrvas
       61 metttgrtsd ysvhsahiae vhdipmdvli rpltpvldet kvaslmetlk dpaqreavpp
      121 idvlwitgre ggnyyysfgg chryeahrrl glptakaklf rstvrdlqay lgastpdlk
//
LOCUS       XP_077505126             762 aa            linear   INV 25-NOV-2025
DEFINITION  calpain-9-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505126
VERSION     XP_077505126.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649000.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..762
                     /product="calpain-9-like isoform X1"
                     /calculated_mol_wt=85779
     Region          109..400
                     /region_name="Peptidase_C2"
                     /note="Calpain family cysteine protease; pfam00648"
                     /db_xref="CDD:459889"
     Region          427..558
                     /region_name="Calpain_III"
                     /note="Calpain large subunit, domain III; pfam01067"
                     /db_xref="CDD:460050"
     Site            order(455..457,464)
                     /site_type="other"
                     /note="acidic loop"
                     /db_xref="CDD:238132"
     Region          594..761
                     /region_name="EFh_PEF"
                     /note="The penta-EF hand (PEF) family; cl25352"
                     /db_xref="CDD:355382"
     Region          594..619
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Site            order(602,610,648,650,652,659,678,680,682,689)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320057"
     Site            order(639,641,651,705,708,711..713,723,727,730..731,
                     734..735,738,748..750,751..753,755..761)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:320057"
     Region          639..668
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          669..698
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          705..732
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          734..761
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     CDS             1..762
                     /gene="LOC144114946"
                     /coded_by="XM_077649000.1:27..2315"
                     /db_xref="GeneID:144114946"
ORIGIN      
        1 mrstpltaaa fsspgipldq pgarhlfpea lpgfcpacsh tparassisp pakcdrrgrd
       61 rtrvfwrgpp avrlssssam gvseggvang hgpgysylkr lcrdrgtlfe dpefppshra
      121 lssknkyasa qvvwmrpyei carpkfiseg ssrfdveqge lgdrwllsai scltstprfl
      181 drvvpsdqgf gpgycglfrf rfwrfgewhe llvddrlpth rgrlfymhsa dpsefwaall
      241 ekayakfygg ydqlqlghtg ralqdltggv aqtfrmahvd ppmafsmvss avprstlmaa
      301 ciipdhhksp arlnnglytq haysvtgiak vrvkqgevtl irlrnpwgrg ewngpwsdrs
      361 wewdslserd rqllglrqpp sdgefwmsfe dftlnfthld lvhigpddwm qesalhskkp
      421 wravlarrrw ragynagggp nhpettgcnp qfhvqipkts sskchmvvsv tqqyvtdalr
      481 dggkrptlhp vgfaiyeipa nltrlttqfv athrpldvtv htttretvtf ftlpagdyiv
      541 lpctsapnce trfllriftd ehsnvwevnd dnliyrnlsl tkngttghpk gpamfsklah
      601 klpceldaym llkilraisr knlclsdtql llekptlelc rhlvmlkdpv ingkisikdv
      661 plilgmlhfw rsvfvkfdrp hkgrtssfnl rpllweagit vsnkvleclv irytkdctit
      721 aeafigalvk lylahdrygq vekklkenti sleemilmtv ys
//
LOCUS       XP_077505127             678 aa            linear   INV 25-NOV-2025
DEFINITION  calpain-9-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505127
VERSION     XP_077505127.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649001.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..678
                     /product="calpain-9-like isoform X2"
                     /calculated_mol_wt=76800
     Region          30..316
                     /region_name="Peptidase_C2"
                     /note="Calpain family cysteine protease; pfam00648"
                     /db_xref="CDD:459889"
     Region          343..474
                     /region_name="Calpain_III"
                     /note="Calpain large subunit, domain III; pfam01067"
                     /db_xref="CDD:460050"
     Site            order(371..373,380)
                     /site_type="other"
                     /note="acidic loop"
                     /db_xref="CDD:238132"
     Region          510..677
                     /region_name="EFh_PEF"
                     /note="The penta-EF hand (PEF) family; cl25352"
                     /db_xref="CDD:355382"
     Region          510..535
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Site            order(518,526,564,566,568,575,594,596,598,605)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320057"
     Site            order(555,557,567,621,624,627..629,639,643,646..647,
                     650..651,654,664..666,667..669,671..677)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:320057"
     Region          555..584
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          585..614
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          621..648
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          650..677
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     CDS             1..678
                     /gene="LOC144114946"
                     /coded_by="XM_077649001.1:202..2238"
                     /db_xref="GeneID:144114946"
ORIGIN      
        1 mgvseggvan ghgpgysylk rlcrdrgtlf edpefppshr alssknkyas aqvvwmrpye
       61 icarpkfise gssrfdveqg elgdrwllsa iscltstprf ldrvvpsdqg fgpgycglfr
      121 frfwrfgewh ellvddrlpt hrgrlfymhs adpsefwaal lekayakfyg gydqlqlght
      181 gralqdltgg vaqtfrmahv dppmafsmvs savprstlma aciipdhhks parlnnglyt
      241 qhaysvtgia kgevtlirlr npwgrgewng pwsdrswewd slserdrqll glrqppsdge
      301 fwmsfedftl nfthldlvhi gpddwmqesa lhskkpwrav larrrwragy nagggpnhpe
      361 ttgcnpqfhv qipktssskc hmvvsvtqqy vtdalrdggk rptlhpvgfa iyeipanltr
      421 lttqfvathr pldvtvhttt retvtfftlp agdyivlpct sapncetrfl lriftdehsn
      481 vwevnddnli yrnlsltkng ttghpkgpam fsklahklpc eldaymllki lraisrknlc
      541 lsdtqlllek ptlelcrhlv mlkdpvingk isikdvplil gmlhfwrsvf vkfdrphkgr
      601 tssfnlrpll weagitvsnk vleclviryt kdctitaeaf igalvklyla hdrygqvekk
      661 lkentislee milmtvys
//
LOCUS       XP_077505128             677 aa            linear   INV 25-NOV-2025
DEFINITION  calpain-9-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077505128
VERSION     XP_077505128.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649002.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..677
                     /product="calpain-9-like isoform X3"
                     /calculated_mol_wt=76751
     Region          30..321
                     /region_name="Peptidase_C2"
                     /note="Calpain family cysteine protease; pfam00648"
                     /db_xref="CDD:459889"
     Region          348..479
                     /region_name="Calpain_III"
                     /note="Calpain large subunit, domain III; pfam01067"
                     /db_xref="CDD:460050"
     Site            order(376..378,385)
                     /site_type="other"
                     /note="acidic loop"
                     /db_xref="CDD:238132"
     Region          515..676
                     /region_name="EFh_PEF"
                     /note="The penta-EF hand (PEF) family; cl25352"
                     /db_xref="CDD:355382"
     Region          515..540
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Site            order(523,531,563,565,567,574,593,595,597,604)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320057"
     Site            order(554,556,566,620,623,626..628,638,642,645..646,
                     649..650,653,663..665,666..668,670..676)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:320057"
     Region          554..583
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          584..613
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          620..647
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          649..676
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     CDS             1..677
                     /gene="LOC144114946"
                     /coded_by="XM_077649002.1:201..2234"
                     /db_xref="GeneID:144114946"
ORIGIN      
        1 mgvseggvan ghgpgysylk rlcrdrgtlf edpefppshr alssknkyas aqvvwmrpye
       61 icarpkfise gssrfdveqg elgdrwllsa iscltstprf ldrvvpsdqg fgpgycglfr
      121 frfwrfgewh ellvddrlpt hrgrlfymhs adpsefwaal lekayakfyg gydqlqlght
      181 gralqdltgg vaqtfrmahv dppmafsmvs savprstlma aciipdhhks parlnnglyt
      241 qhaysvtgia kvrvkqgevt lirlrnpwgr gewngpwsdr swewdslser drqllglrqp
      301 psdgefwmsf edftlnfthl dlvhigpddw mqesalhskk pwravlarrr wragynaggg
      361 pnhpettgcn pqfhvqipkt ssskchmvvs vtqqyvtdal rdggkrptlh pvgfaiyeip
      421 anltrlttqf vathrpldvt vhtttretvt fftlpagdyi vlpctsapnc etrfllrift
      481 dehsnvwevn ddnliyrnls ltkngttghp kgpamfskla hklpcelday mllkilrais
      541 rntqlllekp tlelcrhlvm lkdpvingki sikdvplilg mlhfwrsvfv kfdrphkgrt
      601 ssfnlrpllw eagitvsnkv leclvirytk dctitaeafi galvklylah drygqvekkl
      661 kentisleem ilmtvys
//
LOCUS       XP_077505129             755 aa            linear   INV 25-NOV-2025
DEFINITION  calpain-9-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077505129
VERSION     XP_077505129.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649003.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..755
                     /product="calpain-9-like isoform X4"
                     /calculated_mol_wt=85068
     Region          102..393
                     /region_name="Peptidase_C2"
                     /note="Calpain family cysteine protease; pfam00648"
                     /db_xref="CDD:459889"
     Region          420..551
                     /region_name="Calpain_III"
                     /note="Calpain large subunit, domain III; pfam01067"
                     /db_xref="CDD:460050"
     Site            order(448..450,457)
                     /site_type="other"
                     /note="acidic loop"
                     /db_xref="CDD:238132"
     Region          587..754
                     /region_name="EFh_PEF"
                     /note="The penta-EF hand (PEF) family; cl25352"
                     /db_xref="CDD:355382"
     Region          587..612
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Site            order(595,603,641,643,645,652,671,673,675,682)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320057"
     Site            order(632,634,644,698,701,704..706,716,720,723..724,
                     727..728,731,741..743,744..746,748..754)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:320057"
     Region          632..661
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          662..691
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          698..725
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          727..754
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     CDS             1..755
                     /gene="LOC144114946"
                     /coded_by="XM_077649003.1:27..2294"
                     /db_xref="GeneID:144114946"
ORIGIN      
        1 mrstpltaaa fsspgipldq pgarhlfpea lpgfcpacsh tpalwprend sngdfwrsqr
       61 gppavrlsss samgvseggv anghgpgysy lkrlcrdrgt lfedpefpps hralssknky
      121 asaqvvwmrp yeicarpkfi segssrfdve qgelgdrwll saiscltstp rfldrvvpsd
      181 qgfgpgycgl frfrfwrfge whellvddrl pthrgrlfym hsadpsefwa allekayakf
      241 yggydqlqlg htgralqdlt ggvaqtfrma hvdppmafsm vssavprstl maaciipdhh
      301 ksparlnngl ytqhaysvtg iakvrvkqge vtlirlrnpw grgewngpws drswewdsls
      361 erdrqllglr qppsdgefwm sfedftlnft hldlvhigpd dwmqesalhs kkpwravlar
      421 rrwragynag ggpnhpettg cnpqfhvqip ktssskchmv vsvtqqyvtd alrdggkrpt
      481 lhpvgfaiye ipanltrltt qfvathrpld vtvhtttret vtfftlpagd yivlpctsap
      541 ncetrfllri ftdehsnvwe vnddnliyrn lsltkngttg hpkgpamfsk lahklpceld
      601 aymllkilra isrknlclsd tqlllekptl elcrhlvmlk dpvingkisi kdvplilgml
      661 hfwrsvfvkf drphkgrtss fnlrpllwea gitvsnkvle clvirytkdc titaeafiga
      721 lvklylahdr ygqvekklke ntisleemil mtvys
//
LOCUS       XP_077505130             739 aa            linear   INV 25-NOV-2025
DEFINITION  calpain-9-like isoform X5 [Amblyomma americanum].
ACCESSION   XP_077505130
VERSION     XP_077505130.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649004.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..739
                     /product="calpain-9-like isoform X5"
                     /calculated_mol_wt=83136
     Region          86..377
                     /region_name="Peptidase_C2"
                     /note="Calpain family cysteine protease; pfam00648"
                     /db_xref="CDD:459889"
     Region          404..535
                     /region_name="Calpain_III"
                     /note="Calpain large subunit, domain III; pfam01067"
                     /db_xref="CDD:460050"
     Site            order(432..434,441)
                     /site_type="other"
                     /note="acidic loop"
                     /db_xref="CDD:238132"
     Region          571..738
                     /region_name="EFh_PEF"
                     /note="The penta-EF hand (PEF) family; cl25352"
                     /db_xref="CDD:355382"
     Region          571..596
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Site            order(579,587,625,627,629,636,655,657,659,666)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320057"
     Site            order(616,618,628,682,685,688..690,700,704,707..708,
                     711..712,715,725..727,728..730,732..738)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:320057"
     Region          616..645
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          646..675
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          682..709
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          711..738
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     CDS             1..739
                     /gene="LOC144114946"
                     /coded_by="XM_077649004.1:27..2246"
                     /db_xref="GeneID:144114946"
ORIGIN      
        1 mrstpltaaa fsspgipldq pgarhlfpea lpgfcpacsh tpqrgppavr lssssamgvs
       61 eggvanghgp gysylkrlcr drgtlfedpe fppshralss knkyasaqvv wmrpyeicar
      121 pkfisegssr fdveqgelgd rwllsaiscl tstprfldrv vpsdqgfgpg ycglfrfrfw
      181 rfgewhellv ddrlpthrgr lfymhsadps efwaalleka yakfyggydq lqlghtgral
      241 qdltggvaqt frmahvdppm afsmvssavp rstlmaacii pdhhksparl nnglytqhay
      301 svtgiakvrv kqgevtlirl rnpwgrgewn gpwsdrswew dslserdrql lglrqppsdg
      361 efwmsfedft lnfthldlvh igpddwmqes alhskkpwra vlarrrwrag ynagggpnhp
      421 ettgcnpqfh vqipktsssk chmvvsvtqq yvtdalrdgg krptlhpvgf aiyeipanlt
      481 rlttqfvath rpldvtvhtt tretvtfftl pagdyivlpc tsapncetrf llriftdehs
      541 nvwevnddnl iyrnlsltkn gttghpkgpa mfsklahklp celdaymllk ilraisrknl
      601 clsdtqllle kptlelcrhl vmlkdpving kisikdvpli lgmlhfwrsv fvkfdrphkg
      661 rtssfnlrpl lweagitvsn kvleclviry tkdctitaea figalvklyl ahdrygqvek
      721 klkentisle emilmtvys
//
LOCUS       XP_077505131             704 aa            linear   INV 25-NOV-2025
DEFINITION  calpain-9-like isoform X6 [Amblyomma americanum].
ACCESSION   XP_077505131
VERSION     XP_077505131.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649005.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..704
                     /product="calpain-9-like isoform X6"
                     /calculated_mol_wt=79498
     Region          51..342
                     /region_name="Peptidase_C2"
                     /note="Calpain family cysteine protease; pfam00648"
                     /db_xref="CDD:459889"
     Region          369..500
                     /region_name="Calpain_III"
                     /note="Calpain large subunit, domain III; pfam01067"
                     /db_xref="CDD:460050"
     Site            order(397..399,406)
                     /site_type="other"
                     /note="acidic loop"
                     /db_xref="CDD:238132"
     Region          536..703
                     /region_name="EFh_PEF"
                     /note="The penta-EF hand (PEF) family; cl25352"
                     /db_xref="CDD:355382"
     Region          536..561
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Site            order(544,552,590,592,594,601,620,622,624,631)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320057"
     Site            order(581,583,593,647,650,653..655,665,669,672..673,
                     676..677,680,690..692,693..695,697..703)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:320057"
     Region          581..610
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          611..640
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          647..674
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          676..703
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     CDS             1..704
                     /gene="LOC144114946"
                     /coded_by="XM_077649005.1:96..2210"
                     /db_xref="GeneID:144114946"
ORIGIN      
        1 matkgaemrg ppavrlssss amgvseggva nghgpgysyl krlcrdrgtl fedpefppsh
       61 ralssknkya saqvvwmrpy eicarpkfis egssrfdveq gelgdrwlls aiscltstpr
      121 fldrvvpsdq gfgpgycglf rfrfwrfgew hellvddrlp thrgrlfymh sadpsefwaa
      181 llekayakfy ggydqlqlgh tgralqdltg gvaqtfrmah vdppmafsmv ssavprstlm
      241 aaciipdhhk sparlnngly tqhaysvtgi akvrvkqgev tlirlrnpwg rgewngpwsd
      301 rswewdslse rdrqllglrq ppsdgefwms fedftlnfth ldlvhigpdd wmqesalhsk
      361 kpwravlarr rwragynagg gpnhpettgc npqfhvqipk tssskchmvv svtqqyvtda
      421 lrdggkrptl hpvgfaiyei panltrlttq fvathrpldv tvhtttretv tfftlpagdy
      481 ivlpctsapn cetrfllrif tdehsnvwev nddnliyrnl sltkngttgh pkgpamfskl
      541 ahklpcelda ymllkilrai srknlclsdt qlllekptle lcrhlvmlkd pvingkisik
      601 dvplilgmlh fwrsvfvkfd rphkgrtssf nlrpllweag itvsnkvlec lvirytkdct
      661 itaeafigal vklylahdry gqvekklken tisleemilm tvys
//
LOCUS       XP_077505132             683 aa            linear   INV 25-NOV-2025
DEFINITION  calpain-9-like isoform X7 [Amblyomma americanum].
ACCESSION   XP_077505132
VERSION     XP_077505132.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649006.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..683
                     /product="calpain-9-like isoform X7"
                     /calculated_mol_wt=77411
     Region          30..321
                     /region_name="Peptidase_C2"
                     /note="Calpain family cysteine protease; pfam00648"
                     /db_xref="CDD:459889"
     Region          348..479
                     /region_name="Calpain_III"
                     /note="Calpain large subunit, domain III; pfam01067"
                     /db_xref="CDD:460050"
     Site            order(376..378,385)
                     /site_type="other"
                     /note="acidic loop"
                     /db_xref="CDD:238132"
     Region          515..682
                     /region_name="EFh_PEF"
                     /note="The penta-EF hand (PEF) family; cl25352"
                     /db_xref="CDD:355382"
     Region          515..540
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Site            order(523,531,569,571,573,580,599,601,603,610)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320057"
     Site            order(560,562,572,626,629,632..634,644,648,651..652,
                     655..656,659,669..671,672..674,676..682)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:320057"
     Region          560..589
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          590..619
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          626..653
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          655..682
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     CDS             1..683
                     /gene="LOC144114946"
                     /coded_by="XM_077649006.1:388..2439"
                     /db_xref="GeneID:144114946"
ORIGIN      
        1 mgvseggvan ghgpgysylk rlcrdrgtlf edpefppshr alssknkyas aqvvwmrpye
       61 icarpkfise gssrfdveqg elgdrwllsa iscltstprf ldrvvpsdqg fgpgycglfr
      121 frfwrfgewh ellvddrlpt hrgrlfymhs adpsefwaal lekayakfyg gydqlqlght
      181 gralqdltgg vaqtfrmahv dppmafsmvs savprstlma aciipdhhks parlnnglyt
      241 qhaysvtgia kvrvkqgevt lirlrnpwgr gewngpwsdr swewdslser drqllglrqp
      301 psdgefwmsf edftlnfthl dlvhigpddw mqesalhskk pwravlarrr wragynaggg
      361 pnhpettgcn pqfhvqipkt ssskchmvvs vtqqyvtdal rdggkrptlh pvgfaiyeip
      421 anltrlttqf vathrpldvt vhtttretvt fftlpagdyi vlpctsapnc etrfllrift
      481 dehsnvwevn ddnliyrnls ltkngttghp kgpamfskla hklpcelday mllkilrais
      541 rknlclsdtq lllekptlel crhlvmlkdp vingkisikd vplilgmlhf wrsvfvkfdr
      601 phkgrtssfn lrpllweagi tvsnkvlecl virytkdcti taeafigalv klylahdryg
      661 qvekklkent isleemilmt vys
//
LOCUS       XP_077505133             683 aa            linear   INV 25-NOV-2025
DEFINITION  calpain-9-like isoform X7 [Amblyomma americanum].
ACCESSION   XP_077505133
VERSION     XP_077505133.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649007.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..683
                     /product="calpain-9-like isoform X7"
                     /calculated_mol_wt=77411
     Region          30..321
                     /region_name="Peptidase_C2"
                     /note="Calpain family cysteine protease; pfam00648"
                     /db_xref="CDD:459889"
     Region          348..479
                     /region_name="Calpain_III"
                     /note="Calpain large subunit, domain III; pfam01067"
                     /db_xref="CDD:460050"
     Site            order(376..378,385)
                     /site_type="other"
                     /note="acidic loop"
                     /db_xref="CDD:238132"
     Region          515..682
                     /region_name="EFh_PEF"
                     /note="The penta-EF hand (PEF) family; cl25352"
                     /db_xref="CDD:355382"
     Region          515..540
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Site            order(523,531,569,571,573,580,599,601,603,610)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320057"
     Site            order(560,562,572,626,629,632..634,644,648,651..652,
                     655..656,659,669..671,672..674,676..682)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:320057"
     Region          560..589
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          590..619
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          626..653
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          655..682
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     CDS             1..683
                     /gene="LOC144114946"
                     /coded_by="XM_077649007.1:165..2216"
                     /db_xref="GeneID:144114946"
ORIGIN      
        1 mgvseggvan ghgpgysylk rlcrdrgtlf edpefppshr alssknkyas aqvvwmrpye
       61 icarpkfise gssrfdveqg elgdrwllsa iscltstprf ldrvvpsdqg fgpgycglfr
      121 frfwrfgewh ellvddrlpt hrgrlfymhs adpsefwaal lekayakfyg gydqlqlght
      181 gralqdltgg vaqtfrmahv dppmafsmvs savprstlma aciipdhhks parlnnglyt
      241 qhaysvtgia kvrvkqgevt lirlrnpwgr gewngpwsdr swewdslser drqllglrqp
      301 psdgefwmsf edftlnfthl dlvhigpddw mqesalhskk pwravlarrr wragynaggg
      361 pnhpettgcn pqfhvqipkt ssskchmvvs vtqqyvtdal rdggkrptlh pvgfaiyeip
      421 anltrlttqf vathrpldvt vhtttretvt fftlpagdyi vlpctsapnc etrfllrift
      481 dehsnvwevn ddnliyrnls ltkngttghp kgpamfskla hklpcelday mllkilrais
      541 rknlclsdtq lllekptlel crhlvmlkdp vingkisikd vplilgmlhf wrsvfvkfdr
      601 phkgrtssfn lrpllweagi tvsnkvlecl virytkdcti taeafigalv klylahdryg
      661 qvekklkent isleemilmt vys
//
LOCUS       XP_077505134             683 aa            linear   INV 25-NOV-2025
DEFINITION  calpain-9-like isoform X7 [Amblyomma americanum].
ACCESSION   XP_077505134
VERSION     XP_077505134.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649008.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..683
                     /product="calpain-9-like isoform X7"
                     /calculated_mol_wt=77411
     Region          30..321
                     /region_name="Peptidase_C2"
                     /note="Calpain family cysteine protease; pfam00648"
                     /db_xref="CDD:459889"
     Region          348..479
                     /region_name="Calpain_III"
                     /note="Calpain large subunit, domain III; pfam01067"
                     /db_xref="CDD:460050"
     Site            order(376..378,385)
                     /site_type="other"
                     /note="acidic loop"
                     /db_xref="CDD:238132"
     Region          515..682
                     /region_name="EFh_PEF"
                     /note="The penta-EF hand (PEF) family; cl25352"
                     /db_xref="CDD:355382"
     Region          515..540
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Site            order(523,531,569,571,573,580,599,601,603,610)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320057"
     Site            order(560,562,572,626,629,632..634,644,648,651..652,
                     655..656,659,669..671,672..674,676..682)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:320057"
     Region          560..589
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          590..619
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          626..653
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          655..682
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     CDS             1..683
                     /gene="LOC144114946"
                     /coded_by="XM_077649008.1:140..2191"
                     /db_xref="GeneID:144114946"
ORIGIN      
        1 mgvseggvan ghgpgysylk rlcrdrgtlf edpefppshr alssknkyas aqvvwmrpye
       61 icarpkfise gssrfdveqg elgdrwllsa iscltstprf ldrvvpsdqg fgpgycglfr
      121 frfwrfgewh ellvddrlpt hrgrlfymhs adpsefwaal lekayakfyg gydqlqlght
      181 gralqdltgg vaqtfrmahv dppmafsmvs savprstlma aciipdhhks parlnnglyt
      241 qhaysvtgia kvrvkqgevt lirlrnpwgr gewngpwsdr swewdslser drqllglrqp
      301 psdgefwmsf edftlnfthl dlvhigpddw mqesalhskk pwravlarrr wragynaggg
      361 pnhpettgcn pqfhvqipkt ssskchmvvs vtqqyvtdal rdggkrptlh pvgfaiyeip
      421 anltrlttqf vathrpldvt vhtttretvt fftlpagdyi vlpctsapnc etrfllrift
      481 dehsnvwevn ddnliyrnls ltkngttghp kgpamfskla hklpcelday mllkilrais
      541 rknlclsdtq lllekptlel crhlvmlkdp vingkisikd vplilgmlhf wrsvfvkfdr
      601 phkgrtssfn lrpllweagi tvsnkvlecl virytkdcti taeafigalv klylahdryg
      661 qvekklkent isleemilmt vys
//
LOCUS       XP_077505135             678 aa            linear   INV 25-NOV-2025
DEFINITION  calpain-9-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505135
VERSION     XP_077505135.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649009.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..678
                     /product="calpain-9-like isoform X2"
                     /calculated_mol_wt=76800
     Region          30..316
                     /region_name="Peptidase_C2"
                     /note="Calpain family cysteine protease; pfam00648"
                     /db_xref="CDD:459889"
     Region          343..474
                     /region_name="Calpain_III"
                     /note="Calpain large subunit, domain III; pfam01067"
                     /db_xref="CDD:460050"
     Site            order(371..373,380)
                     /site_type="other"
                     /note="acidic loop"
                     /db_xref="CDD:238132"
     Region          510..677
                     /region_name="EFh_PEF"
                     /note="The penta-EF hand (PEF) family; cl25352"
                     /db_xref="CDD:355382"
     Region          510..535
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Site            order(518,526,564,566,568,575,594,596,598,605)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320057"
     Site            order(555,557,567,621,624,627..629,639,643,646..647,
                     650..651,654,664..666,667..669,671..677)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:320057"
     Region          555..584
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          585..614
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          621..648
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          650..677
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     CDS             1..678
                     /gene="LOC144114946"
                     /coded_by="XM_077649009.1:184..2220"
                     /db_xref="GeneID:144114946"
ORIGIN      
        1 mgvseggvan ghgpgysylk rlcrdrgtlf edpefppshr alssknkyas aqvvwmrpye
       61 icarpkfise gssrfdveqg elgdrwllsa iscltstprf ldrvvpsdqg fgpgycglfr
      121 frfwrfgewh ellvddrlpt hrgrlfymhs adpsefwaal lekayakfyg gydqlqlght
      181 gralqdltgg vaqtfrmahv dppmafsmvs savprstlma aciipdhhks parlnnglyt
      241 qhaysvtgia kgevtlirlr npwgrgewng pwsdrswewd slserdrqll glrqppsdge
      301 fwmsfedftl nfthldlvhi gpddwmqesa lhskkpwrav larrrwragy nagggpnhpe
      361 ttgcnpqfhv qipktssskc hmvvsvtqqy vtdalrdggk rptlhpvgfa iyeipanltr
      421 lttqfvathr pldvtvhttt retvtfftlp agdyivlpct sapncetrfl lriftdehsn
      481 vwevnddnli yrnlsltkng ttghpkgpam fsklahklpc eldaymllki lraisrknlc
      541 lsdtqlllek ptlelcrhlv mlkdpvingk isikdvplil gmlhfwrsvf vkfdrphkgr
      601 tssfnlrpll weagitvsnk vleclviryt kdctitaeaf igalvklyla hdrygqvekk
      661 lkentislee milmtvys
//
LOCUS       XP_077505136             677 aa            linear   INV 25-NOV-2025
DEFINITION  calpain-9-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077505136
VERSION     XP_077505136.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649010.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..677
                     /product="calpain-9-like isoform X3"
                     /calculated_mol_wt=76751
     Region          30..321
                     /region_name="Peptidase_C2"
                     /note="Calpain family cysteine protease; pfam00648"
                     /db_xref="CDD:459889"
     Region          348..479
                     /region_name="Calpain_III"
                     /note="Calpain large subunit, domain III; pfam01067"
                     /db_xref="CDD:460050"
     Site            order(376..378,385)
                     /site_type="other"
                     /note="acidic loop"
                     /db_xref="CDD:238132"
     Region          515..676
                     /region_name="EFh_PEF"
                     /note="The penta-EF hand (PEF) family; cl25352"
                     /db_xref="CDD:355382"
     Region          515..540
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Site            order(523,531,563,565,567,574,593,595,597,604)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320057"
     Site            order(554,556,566,620,623,626..628,638,642,645..646,
                     649..650,653,663..665,666..668,670..676)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:320057"
     Region          554..583
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          584..613
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          620..647
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          649..676
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     CDS             1..677
                     /gene="LOC144114946"
                     /coded_by="XM_077649010.1:184..2217"
                     /db_xref="GeneID:144114946"
ORIGIN      
        1 mgvseggvan ghgpgysylk rlcrdrgtlf edpefppshr alssknkyas aqvvwmrpye
       61 icarpkfise gssrfdveqg elgdrwllsa iscltstprf ldrvvpsdqg fgpgycglfr
      121 frfwrfgewh ellvddrlpt hrgrlfymhs adpsefwaal lekayakfyg gydqlqlght
      181 gralqdltgg vaqtfrmahv dppmafsmvs savprstlma aciipdhhks parlnnglyt
      241 qhaysvtgia kvrvkqgevt lirlrnpwgr gewngpwsdr swewdslser drqllglrqp
      301 psdgefwmsf edftlnfthl dlvhigpddw mqesalhskk pwravlarrr wragynaggg
      361 pnhpettgcn pqfhvqipkt ssskchmvvs vtqqyvtdal rdggkrptlh pvgfaiyeip
      421 anltrlttqf vathrpldvt vhtttretvt fftlpagdyi vlpctsapnc etrfllrift
      481 dehsnvwevn ddnliyrnls ltkngttghp kgpamfskla hklpcelday mllkilrais
      541 rntqlllekp tlelcrhlvm lkdpvingki sikdvplilg mlhfwrsvfv kfdrphkgrt
      601 ssfnlrpllw eagitvsnkv leclvirytk dctitaeafi galvklylah drygqvekkl
      661 kentisleem ilmtvys
//
LOCUS       XP_077505137             672 aa            linear   INV 25-NOV-2025
DEFINITION  calpain-9-like isoform X8 [Amblyomma americanum].
ACCESSION   XP_077505137
VERSION     XP_077505137.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649011.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..672
                     /product="calpain-9-like isoform X8"
                     /calculated_mol_wt=76141
     Region          30..316
                     /region_name="Peptidase_C2"
                     /note="Calpain family cysteine protease; pfam00648"
                     /db_xref="CDD:459889"
     Region          343..474
                     /region_name="Calpain_III"
                     /note="Calpain large subunit, domain III; pfam01067"
                     /db_xref="CDD:460050"
     Site            order(371..373,380)
                     /site_type="other"
                     /note="acidic loop"
                     /db_xref="CDD:238132"
     Region          510..671
                     /region_name="EFh_PEF"
                     /note="The penta-EF hand (PEF) family; cl25352"
                     /db_xref="CDD:355382"
     Region          510..535
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Site            order(518,526,558,560,562,569,588,590,592,599)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320057"
     Site            order(549,551,561,615,618,621..623,633,637,640..641,
                     644..645,648,658..660,661..663,665..671)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:320057"
     Region          549..578
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          579..608
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          615..642
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     Region          644..671
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320057"
     CDS             1..672
                     /gene="LOC144114946"
                     /coded_by="XM_077649011.1:184..2202"
                     /db_xref="GeneID:144114946"
ORIGIN      
        1 mgvseggvan ghgpgysylk rlcrdrgtlf edpefppshr alssknkyas aqvvwmrpye
       61 icarpkfise gssrfdveqg elgdrwllsa iscltstprf ldrvvpsdqg fgpgycglfr
      121 frfwrfgewh ellvddrlpt hrgrlfymhs adpsefwaal lekayakfyg gydqlqlght
      181 gralqdltgg vaqtfrmahv dppmafsmvs savprstlma aciipdhhks parlnnglyt
      241 qhaysvtgia kgevtlirlr npwgrgewng pwsdrswewd slserdrqll glrqppsdge
      301 fwmsfedftl nfthldlvhi gpddwmqesa lhskkpwrav larrrwragy nagggpnhpe
      361 ttgcnpqfhv qipktssskc hmvvsvtqqy vtdalrdggk rptlhpvgfa iyeipanltr
      421 lttqfvathr pldvtvhttt retvtfftlp agdyivlpct sapncetrfl lriftdehsn
      481 vwevnddnli yrnlsltkng ttghpkgpam fsklahklpc eldaymllki lraisrntql
      541 llekptlelc rhlvmlkdpv ingkisikdv plilgmlhfw rsvfvkfdrp hkgrtssfnl
      601 rpllweagit vsnkvleclv irytkdctit aeafigalvk lylahdrygq vekklkenti
      661 sleemilmtv ys
//
LOCUS       XP_077505138             417 aa            linear   INV 25-NOV-2025
DEFINITION  kip1 ubiquitination-promoting complex subunit 2 isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077505138
VERSION     XP_077505138.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649012.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..417
                     /product="kip1 ubiquitination-promoting complex subunit 2
                     isoform X1"
                     /calculated_mol_wt=46332
     Region          14..93
                     /region_name="Ubl1_cv_Nsp3_N-like"
                     /note="first ubiquitin-like (Ubl) domain located at the
                     N-terminus of coronavirus SARS-CoV non-structural protein
                     3 (Nsp3) and related proteins; cl28922"
                     /db_xref="CDD:475130"
     Region          187..224
                     /region_name="UBA_like_SF"
                     /note="UBA domain-like superfamily; cl21463"
                     /db_xref="CDD:473871"
     Region          292..330
                     /region_name="UBA2_KPC2"
                     /note="UBA2 domain found in Kip1 ubiquitination-promoting
                     complex protein 2 (KPC2) and similar proteins; cd14304"
                     /db_xref="CDD:270489"
     CDS             1..417
                     /gene="Kpc2"
                     /coded_by="XM_077649012.1:167..1420"
                     /db_xref="GeneID:144114948"
ORIGIN      
        1 mlylstglsg acemkisvis stgvewtvei lpeytieklk qmslthfenp ldciklsgyf
       61 klvsvsqarp lcdqssvree klrdgdelll lqrsstfsfn snkssevqkq gipdkavidv
      121 atanvepknf drpamdvpfv pdfhtelrri lvslvelsek llrhhpdien ifknlqskit
      181 ersepeidga alkkllemgf eeqqaiqalr enkmcpmaam ewllakgckq ekevesetsa
      241 kdassaaqac adtqanaeis nsnavpggle qkgivnamle cfqeykrkdf kpnkkamsnl
      301 kemgfsetai ldalrihsnc qeaacewllg drrpkpvdlq vgldtssaiy rsivsnpvvq
      361 lglcspktll allqmlenpg casrwlndsd tapilsqifr iyhaekhsvq lsrplvt
//
LOCUS       XP_077505139             413 aa            linear   INV 25-NOV-2025
DEFINITION  kip1 ubiquitination-promoting complex subunit 2 isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077505139
VERSION     XP_077505139.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649013.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..413
                     /product="kip1 ubiquitination-promoting complex subunit 2
                     isoform X2"
                     /calculated_mol_wt=45902
     Region          14..93
                     /region_name="Ubl1_cv_Nsp3_N-like"
                     /note="first ubiquitin-like (Ubl) domain located at the
                     N-terminus of coronavirus SARS-CoV non-structural protein
                     3 (Nsp3) and related proteins; cl28922"
                     /db_xref="CDD:475130"
     Region          183..220
                     /region_name="UBA_like_SF"
                     /note="UBA domain-like superfamily; cl21463"
                     /db_xref="CDD:473871"
     Region          288..326
                     /region_name="UBA2_KPC2"
                     /note="UBA2 domain found in Kip1 ubiquitination-promoting
                     complex protein 2 (KPC2) and similar proteins; cd14304"
                     /db_xref="CDD:270489"
     CDS             1..413
                     /gene="Kpc2"
                     /coded_by="XM_077649013.1:167..1408"
                     /db_xref="GeneID:144114948"
ORIGIN      
        1 mlylstglsg acemkisvis stgvewtvei lpeytieklk qmslthfenp ldciklsgyf
       61 klvsvsqarp lcdqssvree klrdgdelll lqrsstfsfn snkssevqkq gipdkavidv
      121 atanvepknf drpamdvpfv pdfhtelrri lvslvelsek llrhhpdien ifknlqkrse
      181 peidgaalkk llemgfeeqq aiqalrenkm cpmaamewll akgckqekev esetsakdas
      241 saaqacadtq anaeisnsna vpggleqkgi vnamlecfqe ykrkdfkpnk kamsnlkemg
      301 fsetaildal rihsncqeaa cewllgdrrp kpvdlqvgld tssaiyrsiv snpvvqlglc
      361 spktllallq mlenpgcasr wlndsdtapi lsqifriyha ekhsvqlsrp lvt
//
LOCUS       XP_077505140             277 aa            linear   INV 25-NOV-2025
DEFINITION  proteasome beta2 subunit [Amblyomma americanum].
ACCESSION   XP_077505140
VERSION     XP_077505140.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649014.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..277
                     /product="proteasome beta2 subunit"
                     /calculated_mol_wt=29323
     Region          41..229
                     /region_name="proteasome_beta_type_7"
                     /note="proteasome beta type-7 subunit. The 20S proteasome,
                     multisubunit proteolytic complex, is the central enzyme of
                     nonlysosomal protein degradation in both the cytosol and
                     nucleus. It is composed of 28 subunits arranged as four
                     homoheptameric rings that...; cd03763"
                     /db_xref="CDD:239732"
     Site            order(41,57,59,73,169,206,209..210)
                     /site_type="active"
                     /db_xref="CDD:239732"
     Site            order(59,62,64..69,89,91,106,118,121..122,124..125,128,
                     131,154,156,158..161,164,172..173,175..176,179..181,197,
                     204..207,210..211)
                     /site_type="other"
                     /note="beta subunit interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:239732"
     Region          232..266
                     /region_name="Pr_beta_C"
                     /note="Proteasome beta subunits C terminal; pfam12465"
                     /db_xref="CDD:463597"
     CDS             1..277
                     /gene="Prosbeta2"
                     /coded_by="XM_077649014.1:71..904"
                     /db_xref="GeneID:144114949"
ORIGIN      
        1 maaaatafve pssgfcfenc rrnaflqsqg lplpkatktg ttiagivfkd gvilgadtra
       61 taggfvadkn cdkihylapn iyccgagtaa dtakttrmis sqlelhrlat grivpvctan
      121 rmlkqmlfry qgyisaalvl ggvdttggvl csihphgstd rlpfvtmgsg slaamavfer
      181 dwkpdmslee gkklvrdaia agifndlgsg gnvdlcvitr gglahvrpye eankkgerhl
      241 sykfkkgtta ilganvvpie veetvvkhve veameia
//
LOCUS       XP_077505141             781 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114952 [Amblyomma americanum].
ACCESSION   XP_077505141
VERSION     XP_077505141.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649015.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..781
                     /product="uncharacterized protein LOC144114952"
                     /calculated_mol_wt=86684
     CDS             1..781
                     /gene="LOC144114952"
                     /coded_by="XM_077649015.1:310..2655"
                     /db_xref="GeneID:144114952"
ORIGIN      
        1 meevpcsvtd dfcvpnarav rssaheclnd ssragsnrdt nasplpnnsp lsrppfgsws
       61 sgiraslaec qkgplqmsrg tdvqhvealm srfkehfttv highkyvdge fvcvdcysam
      121 etlveirkaa ikeqddssrl lrdeefmkll vklsmfdspg lpricsvtcs etrtlyetga
      181 yrnlkatvdv hedgageelw kgilgkaaes irlptytvnf hgprlrdgrs smglrtvgrg
      241 alvdvprsnt prtkqkvnpl pksdlrkwsv vrclddrhll vcccaagreg vnslnnilak
      301 chrdsrprkh rpevgeimgl ptssecmlrv lvvdfvsdaa vvwtldegdf lklpccrlvd
      361 llpsfkclpp siglavlpdv naapflgllr ecigffqavv nfhdkgaflq tkirmltslg
      421 vvavlsnlle cfdcatrtas agcltqlcel qdggravlea gclrkvrgrl rklveqhrhd
      481 prteertlvn llnavffrnd elryqyadld dvlllkeiqe rpspdenlsq tvqdcletll
      541 dphdesrrpv spklvamrpe qlknldysvh tphkamehcy glgagpahad pqssvppppg
      601 siltpsghrc yllntlvpfh sdethelqpl ldlelasvhs likficsfln twqlctiycg
      661 itsdglvrgl qlsgkgcdtv rssidsamrv lepcllpqnl dvkfvpvlrt tqdvyeaasf
      721 fvveisvhgv prtvysikni cflrergqsy katsqdvraw iakleetrck qaseeplptn
      781 t
//
LOCUS       XP_077505142             786 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114953 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505142
VERSION     XP_077505142.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649016.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..786
                     /product="uncharacterized protein LOC144114953 isoform X1"
                     /calculated_mol_wt=89542
     CDS             1..786
                     /gene="LOC144114953"
                     /coded_by="XM_077649016.1:503..2863"
                     /db_xref="GeneID:144114953"
ORIGIN      
        1 mprarnvqhv ealvsrfkeh ftkvhnghky vdgefvcvdc ysametlvki rkaaikeqdd
       61 ssrllrdeef mkllvklsmf dspglprics vtcsetrnly etgayrnlka tvnvhedgag
      121 eelwegilgk aaesirlpty tvnfhgshlr ggrssmghia derealvnvp rsntprteek
      181 vnpfpksdlr twsvvrcldd rhllvcccaa gregvnslnn ilaechrdsr rrkhrpevge
      241 imglpkssec mlrvlvvdiv rdaavvwtld egdflklpcr rlvdllpsfk clppsiglav
      301 lpdvnaapll gllrecvrif qavvkfrdkk vfrqrikvrr ltslgvvevl snllecfdca
      361 trmasaacli qlcklqdgdq avfkagclrk vrgrlrklve qhrhdprtee ktlvnllnaa
      421 ffrndelrfq yadqddvvll keiqecpspd eklsravqgc letlldprdq rrrpvspklv
      481 amspeqlknr dysvhtprka mehcygekie ayhlvigfil lmnvlccnyg flvtslpskg
      541 hsrrmckiss ewwhqsrffk drlrvacers gdrqghlrff rdwcatvdqy teflwakvrp
      601 qlpkrkktpq qpgkviilge tpipqqyesl lqmgpkfcmk phlrkedkvt iarsishlaq
      661 dvyqprcvee ctavlsrtqt dkyregygsl vnflhsnylr lltadkegcf vvlpktmfsl
      721 kameavqknf vpvstkpgkv karalrllgg mnldrlkasv nkvkglslki fsqrsstkma
      781 chfgll
//
LOCUS       XP_077505143             707 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114953 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505143
VERSION     XP_077505143.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649017.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..707
                     /product="uncharacterized protein LOC144114953 isoform X2"
                     /calculated_mol_wt=80136
     Region          547..678
                     /region_name="AlbA_2"
                     /note="Putative DNA-binding domain; pfam04326"
                     /db_xref="CDD:461263"
     CDS             1..707
                     /gene="LOC144114953"
                     /coded_by="XM_077649017.1:503..2626"
                     /db_xref="GeneID:144114953"
ORIGIN      
        1 mprarnvqhv ealvsrfkeh ftkvhnghky vdgefvcvdc ysametlvki rkaaikeqdd
       61 ssrllrdeef mkllvklsmf dspglprics vtcsetrnly etgayrnlka tvnvhedgag
      121 eelwegilgk aaesirlpty tvnfhgshlr ggrssmghia derealvnvp rsntprteek
      181 vnpfpksdlr twsvvrcldd rhllvcccaa gregvnslnn ilaechrdsr rrkhrpevge
      241 imglpkssec mlrvlvvdiv rdaavvwtld egdflklpcr rlvdllpsfk clppsiglav
      301 lpdvnaapll gllrecvrif qavvkfrdkk vfrqrikvrr ltslgvvevl snllecfdca
      361 trmasaacli qlcklqdgdq avfkagclrk vrgrlrklve qhrhdprtee ktlvnllnaa
      421 ffrndelrfq yadqddvvll keiqecpspd eklsravqgc letlldprdq rrrpvspklv
      481 amspeqlknr dysvhtprka mehcyglgag pahadpqssv kpppgsiltp sghrcyllnt
      541 lvpfhsdeth elwplldlkq vfvrtvtkfi csflntwqlc tiyfgitsdg lvrgvelthk
      601 drdnvrlsid aavrvlrpcl lpqsldvkfv pmlrtaqdvy eaasffvlei svhgvprtmy
      661 tiksicylre rdqsyeatse dvrawiaklq etyclpaqep lltyqrt
//
LOCUS       XP_077505145             558 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114953 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505145
VERSION     XP_077505145.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649019.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..558
                     /product="uncharacterized protein LOC144114953 isoform X3"
                     /calculated_mol_wt=63449
     Region          <228..294
                     /region_name="Tudor_SF"
                     /note="Tudor domain superfamily; cl02573"
                     /db_xref="CDD:470623"
     Site            order(247,250,252,268,271,273)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:410449"
     CDS             1..558
                     /gene="LOC144114953"
                     /coded_by="XM_077649019.1:503..2179"
                     /db_xref="GeneID:144114953"
ORIGIN      
        1 mprarnvqhv ealvsrfkeh ftkvhnghky vdgefvcvdc ysametlvki rkaaikeqdd
       61 ssrllrdeef mkllvklsmf dspglprics vtcsetrnly etgayrnlka tvnvhedgag
      121 eelwegilgk aaesirlpty tvnfhgshlr ggrssmghia derealvnvp rsntprteek
      181 vnpfpksdlr twsvvrcldd rhllvcccaa gregvnslnn ilaechrdsr rrkhrpevge
      241 imglpkssec mlrvlvvdiv rdaavvwtld egdflklpcr rlvdllpsfk clppsiglav
      301 lpdvnaapll gllrecvrif qavvkfrdkk vfrqrikvrr ltslgvvevl snllecfdca
      361 trmasaacli qlcklqdgdq avfkagclrk vrgrlrklve qhrhdprtee ktlvnllnaa
      421 ffrndelrfq yadqddvvll keiqecpspd eklsravqgc letlldprdq rrrpvspklv
      481 amspeqlknr dysvhtprka mehcygekie ayhlvigfil lmnvlccnyg flvtslvstf
      541 ihiyffavvs nwkmkysk
//
LOCUS       XP_077505146             263 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114954 [Amblyomma americanum].
ACCESSION   XP_077505146
VERSION     XP_077505146.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649020.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..263
                     /product="uncharacterized protein LOC144114954"
                     /calculated_mol_wt=28335
     Region          <55..>197
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     CDS             1..263
                     /gene="LOC144114954"
                     /coded_by="XM_077649020.1:1..792"
                     /db_xref="GeneID:144114954"
ORIGIN      
        1 mpevgslgsp isqslkgrks pevincnhtl glyfhvtdav qtdgclvpka dcevqtsgnd
       61 delllqless ehevnllkss ihricldnaa lgsmykerrg qltkaseich aledqmkrla
      121 eelkatktdk ealqvnkhlk aevsklqekv csltneiaet rdsealkleg ierlyreake
      181 enqfmrravt aasagvtpgh qastavqqgs rpvpaapqdv pkcmptqktp vkaspckgkh
      241 pgsasavpas skksklenkg dss
//
LOCUS       XP_077505147             298 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114955 [Amblyomma americanum].
ACCESSION   XP_077505147
VERSION     XP_077505147.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649021.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..298
                     /product="uncharacterized protein LOC144114955"
                     /calculated_mol_wt=32320
     CDS             1..298
                     /gene="LOC144114955"
                     /coded_by="XM_077649021.1:42..938"
                     /db_xref="GeneID:144114955"
ORIGIN      
        1 medaaeqvvr rreirrkkil ensdarwmki mgqenrrsed vskevsncaq aqttkqelpl
       61 pavppetrcp slgeptrvsy slgdaasqfi sedisgtvag stvsgsrlse trtrsldeta
      121 aangaprlac sqrsasysfp kkvsdaesrs yarcrsdtrg tdegtlswlf ellgpcgpsl
      181 vrawllgmaa tctriwlavg aaalgglqti llpfclleta mysyhkfssk pslsqrplrs
      241 tpaplaflat alvlcgvspk vssrttaclq lvadvmvdla vytfsfvvfh viwddwvg
//
LOCUS       XP_077505148             284 aa            linear   INV 25-NOV-2025
DEFINITION  transcription factor A, mitochondrial-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505148
VERSION     XP_077505148.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649022.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..284
                     /product="transcription factor A, mitochondrial-like
                     isoform X1"
                     /calculated_mol_wt=31852
     Region          55..122
                     /region_name="HMG_box"
                     /note="HMG (high mobility group) box; pfam00505"
                     /db_xref="CDD:459837"
     Region          160..250
                     /region_name="HMG-box_SF"
                     /note="high mobility group (HMG)-box domain superfamily;
                     cl00082"
                     /db_xref="CDD:469606"
     Site            order(162,164..168,171..172,202,212..213,216,219..220,227,
                     231,234,238,241)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438789"
     CDS             1..284
                     /gene="LOC144114956"
                     /coded_by="XM_077649022.1:63..917"
                     /db_xref="GeneID:144114956"
ORIGIN      
        1 malsmsaalr gffankqaig rslcgassct rllqdsargl atqkhgntst fppapkrpps
       61 gfilfaadar kavlkenpal tptevikkva gkwrtvdevt kskyaamsre rfeqyekeka
      121 aytsvltdqq kealaevrln kklkvskrrl ndklkeldrp kgarsayvlf stemrkklqd
      181 kpaksaagsr vsawflgkll sksevpspqe imaqigamwk qlpeekkqpy lhqadqdklr
      241 yeqemkawsq rlekegqsgv lndlrqdire irkskqgsar knit
//
LOCUS       XP_077505149             284 aa            linear   INV 25-NOV-2025
DEFINITION  transcription factor A, mitochondrial-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505149
VERSION     XP_077505149.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649023.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..284
                     /product="transcription factor A, mitochondrial-like
                     isoform X1"
                     /calculated_mol_wt=31852
     Region          55..122
                     /region_name="HMG_box"
                     /note="HMG (high mobility group) box; pfam00505"
                     /db_xref="CDD:459837"
     Region          160..250
                     /region_name="HMG-box_SF"
                     /note="high mobility group (HMG)-box domain superfamily;
                     cl00082"
                     /db_xref="CDD:469606"
     Site            order(162,164..168,171..172,202,212..213,216,219..220,227,
                     231,234,238,241)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438789"
     CDS             1..284
                     /gene="LOC144114956"
                     /coded_by="XM_077649023.1:63..917"
                     /db_xref="GeneID:144114956"
ORIGIN      
        1 malsmsaalr gffankqaig rslcgassct rllqdsargl atqkhgntst fppapkrpps
       61 gfilfaadar kavlkenpal tptevikkva gkwrtvdevt kskyaamsre rfeqyekeka
      121 aytsvltdqq kealaevrln kklkvskrrl ndklkeldrp kgarsayvlf stemrkklqd
      181 kpaksaagsr vsawflgkll sksevpspqe imaqigamwk qlpeekkqpy lhqadqdklr
      241 yeqemkawsq rlekegqsgv lndlrqdire irkskqgsar knit
//
LOCUS       XP_077505150             259 aa            linear   INV 25-NOV-2025
DEFINITION  transcription factor A, mitochondrial-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505150
VERSION     XP_077505150.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649024.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..259
                     /product="transcription factor A, mitochondrial-like
                     isoform X2"
                     /calculated_mol_wt=29267
     Region          55..122
                     /region_name="HMG_box"
                     /note="HMG (high mobility group) box; pfam00505"
                     /db_xref="CDD:459837"
     Region          160..225
                     /region_name="HMG-box_SF"
                     /note="high mobility group (HMG)-box domain superfamily;
                     cl00082"
                     /db_xref="CDD:469606"
     Site            order(162,164..168,171..172,179,187..188,191,194..195,202,
                     206,209,213,216)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438789"
     CDS             1..259
                     /gene="LOC144114956"
                     /coded_by="XM_077649024.1:63..842"
                     /db_xref="GeneID:144114956"
ORIGIN      
        1 malsmsaalr gffankqaig rslcgassct rllqdsargl atqkhgntst fppapkrpps
       61 gfilfaadar kavlkenpal tptevikkva gkwrtvdevt kskyaamsre rfeqyekeka
      121 aytsvltdqq kealaevrln kklkvskrrl ndklkeldrp kgarsayvlf stemrkklqd
      181 kpakeimaqi gamwkqlpee kkqpylhqad qdklryeqem kawsqrleke gqsgvlndlr
      241 qdireirksk qgsarknit
//
LOCUS       XP_077505151             242 aa            linear   INV 25-NOV-2025
DEFINITION  transcription factor A, mitochondrial-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505151
VERSION     XP_077505151.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649025.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..242
                     /product="transcription factor A, mitochondrial-like
                     isoform X1"
                     /calculated_mol_wt=28007
     Region          25..83
                     /region_name="HMG-box_SF"
                     /note="high mobility group (HMG)-box domain superfamily;
                     cd00084"
                     /db_xref="CDD:438789"
     Site            order(25,27..31,34..35,42,54..55,58,61..62,69,73,76,80,83)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438789"
     Region          94..>198
                     /region_name="NHP6B"
                     /note="Chromatin-associated proteins containing the HMG
                     domain [Chromatin structure and dynamics]; COG5648"
                     /db_xref="CDD:227935"
     Region          143..194
                     /region_name="HMG-box_SF"
                     /note="high mobility group (HMG)-box domain superfamily;
                     cd00084"
                     /db_xref="CDD:438789"
     Site            order(144..148,151..152,165..166,169,172..173,180,184,187,
                     191,194)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438789"
     CDS             1..242
                     /gene="LOC144114957"
                     /coded_by="XM_077649025.1:238..966"
                     /db_xref="GeneID:144114957"
ORIGIN      
        1 mlrvwfsfrk lsskadtrcl ptppkrlsgy slflktvwps lsqqhpgekf sqlvsiaakk
       61 wkaadsqtkq lygekardis aryekeysey lsslnfehil atggktkahl ktksrrlenv
      121 rsfvnnhgrs rttlrrlkkp rsprsayaff ciearkpnlk lseeakqlae kwsalsdsek
      181 qvyqqraeed krryhdamid wemcmqqtgn skilqeyfen nvdvakkhla kqlthceesl
      241 gg
//
LOCUS       XP_077505152             229 aa            linear   INV 25-NOV-2025
DEFINITION  transcription factor A, mitochondrial-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505152
VERSION     XP_077505152.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649026.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..229
                     /product="transcription factor A, mitochondrial-like
                     isoform X2"
                     /calculated_mol_wt=26495
     Region          25..83
                     /region_name="HMG-box_SF"
                     /note="high mobility group (HMG)-box domain superfamily;
                     cd00084"
                     /db_xref="CDD:438789"
     Site            order(25,27..31,34..35,42,54..55,58,61..62,69,73,76,80,83)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438789"
     Region          107..201
                     /region_name="HMG-box_SF"
                     /note="high mobility group (HMG)-box domain superfamily;
                     cl00082"
                     /db_xref="CDD:469606"
     Site            order(129,131..135,138..139,144,152..153,156,159..160,167,
                     171,174,178,181)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438789"
     CDS             1..229
                     /gene="LOC144114957"
                     /coded_by="XM_077649026.1:228..917"
                     /db_xref="GeneID:144114957"
ORIGIN      
        1 mlrvwfsfrk lsskadtrcl ptppkrlsgy slflktvwps lsqqhpgekf sqlvsiaakk
       61 wkaadsqtkq lygekardis aryekeysey lsslnfehil atggktkahl ktksrrlent
      121 lrrlkkprsp rsayaffcie arkpnlklse eakqlaekws alsdsekqvy qqraeedkrr
      181 yhdamidwem cmqqtgnski lqeyfennvd vakkhlakql thceeslgg
//
LOCUS       XP_077505153             198 aa            linear   INV 25-NOV-2025
DEFINITION  transcription factor A, mitochondrial-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505153
VERSION     XP_077505153.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649027.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..198
                     /product="transcription factor A, mitochondrial-like
                     isoform X3"
                     /calculated_mol_wt=22513
     Region          25..83
                     /region_name="HMG-box_SF"
                     /note="high mobility group (HMG)-box domain superfamily;
                     cd00084"
                     /db_xref="CDD:438789"
     Site            order(25,27..31,34..35,42,54..55,58,61..62,69,73,76,80,83)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438789"
     Region          94..>181
                     /region_name="NHP6B"
                     /note="Chromatin-associated proteins containing the HMG
                     domain [Chromatin structure and dynamics]; COG5648"
                     /db_xref="CDD:227935"
     Region          143..>181
                     /region_name="HMG-box_SF"
                     /note="high mobility group (HMG)-box domain superfamily;
                     cd00084"
                     /db_xref="CDD:438789"
     CDS             1..198
                     /gene="LOC144114957"
                     /coded_by="XM_077649027.1:241..837"
                     /db_xref="GeneID:144114957"
ORIGIN      
        1 mlrvwfsfrk lsskadtrcl ptppkrlsgy slflktvwps lsqqhpgekf sqlvsiaakk
       61 wkaadsqtkq lygekardis aryekeysey lsslnfehil atggktkahl ktksrrlenv
      121 rsfvnnhgrs rttlrrlkkp rsprsayaff ciearkpnlk lseeakqlae kwsalsdsek
      181 qgatvvqkql cstgetqt
//
LOCUS       XP_077505154             185 aa            linear   INV 25-NOV-2025
DEFINITION  transcription factor A, mitochondrial-like isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505154
VERSION     XP_077505154.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649028.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..185
                     /product="transcription factor A, mitochondrial-like
                     isoform X4"
                     /calculated_mol_wt=21001
     Region          25..83
                     /region_name="HMG-box_SF"
                     /note="high mobility group (HMG)-box domain superfamily;
                     cd00084"
                     /db_xref="CDD:438789"
     Site            order(25,27..31,34..35,42,54..55,58,61..62,69,73,76,80,83)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438789"
     Region          130..>168
                     /region_name="HMG-box_SF"
                     /note="high mobility group (HMG)-box domain superfamily;
                     cd00084"
                     /db_xref="CDD:438789"
     CDS             1..185
                     /gene="LOC144114957"
                     /coded_by="XM_077649028.1:230..787"
                     /db_xref="GeneID:144114957"
ORIGIN      
        1 mlrvwfsfrk lsskadtrcl ptppkrlsgy slflktvwps lsqqhpgekf sqlvsiaakk
       61 wkaadsqtkq lygekardis aryekeysey lsslnfehil atggktkahl ktksrrlent
      121 lrrlkkprsp rsayaffcie arkpnlklse eakqlaekws alsdsekqga tvvqkqlcst
      181 getqt
//
LOCUS       XP_077505155             131 aa            linear   INV 25-NOV-2025
DEFINITION  proteasome inhibitor PI31 subunit-like [Amblyomma americanum].
ACCESSION   XP_077505155
VERSION     XP_077505155.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649029.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..131
                     /product="proteasome inhibitor PI31 subunit-like"
                     /calculated_mol_wt=14094
     CDS             1..131
                     /gene="LOC144114959"
                     /coded_by="XM_077649029.1:327..722"
                     /db_xref="GeneID:144114959"
ORIGIN      
        1 meccdsgsei igvrdaspta sqesllhmpr trvgiewpqt pqyvplgqrd lnpffggglg
       61 ggmimdqrhl prpprpdlra tapglprgav ppgarfdpfg pphpdhpadf rfagpnpdhl
      121 arppddddmf s
//
LOCUS       XP_077505156             209 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114960 [Amblyomma americanum].
ACCESSION   XP_077505156
VERSION     XP_077505156.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649030.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..209
                     /product="uncharacterized protein LOC144114960"
                     /calculated_mol_wt=22919
     Region          <7..57
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..209
                     /gene="LOC144114960"
                     /coded_by="XM_077649030.1:448..1077"
                     /db_xref="GeneID:144114960"
ORIGIN      
        1 msflttdllq kwlsairtpl pnlhigrric srhfeqscfy sghtrhflkp davptifqkv
       61 qseqvastsc gaisglqslp rmslqkrkre haspeatssq tmsspssraq lplrksprlq
      121 aisvrphlsq pvtspipksp ckhlhtptrk slrkrkqdms apqtpssvaa spwksprkcl
      181 qtatpvcass rmhsedqgat spakaavkv
//
LOCUS       XP_077505158             407 aa            linear   INV 25-NOV-2025
DEFINITION  sterol carrier protein 2-like [Amblyomma americanum].
ACCESSION   XP_077505158
VERSION     XP_077505158.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649032.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..407
                     /product="sterol carrier protein 2-like"
                     /calculated_mol_wt=43138
     Region          9..399
                     /region_name="PRK08256"
                     /note="lipid-transfer protein; Provisional"
                     /db_xref="CDD:181327"
     CDS             1..407
                     /gene="LOC144114962"
                     /coded_by="XM_077649032.1:195..1418"
                     /db_xref="GeneID:144114962"
ORIGIN      
        1 maaaerggrr vfvvgvgmtk fakpgdkkrd ypvlakeaat aalqdcrmey gdveqaccgy
       61 vygdstcgqr cvyelgmtgi pvynvnnncs tgstalllak qlveggladv vlalgfekmd
      121 rgsltskyrd ranpvekhak vlaathglap apmaaqmfga aglehmakyg strehlakia
      181 qknhrhsvhn pnsqfreeht leeilespvv fgpltklqcc ptsdgaaaav lvseavvrrl
      241 qleaqavevl siematdlps afadnsciem vgfsmtrkaa qqafrraglt pddvqvvelh
      301 dcfsanella yealglcpvg kaaelvdrgd ntyggkyvin psggliskgh plgatglaqc
      361 aelswqlrgl agprqvpgar valqhniglg gavvvalyrl gfpraal
//
LOCUS       XP_077505159             407 aa            linear   INV 25-NOV-2025
DEFINITION  sterol carrier protein 2-like [Amblyomma americanum].
ACCESSION   XP_077505159
VERSION     XP_077505159.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649033.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..407
                     /product="sterol carrier protein 2-like"
                     /calculated_mol_wt=43138
     Region          9..399
                     /region_name="PRK08256"
                     /note="lipid-transfer protein; Provisional"
                     /db_xref="CDD:181327"
     CDS             1..407
                     /gene="LOC144114962"
                     /coded_by="XM_077649033.1:303..1526"
                     /db_xref="GeneID:144114962"
ORIGIN      
        1 maaaerggrr vfvvgvgmtk fakpgdkkrd ypvlakeaat aalqdcrmey gdveqaccgy
       61 vygdstcgqr cvyelgmtgi pvynvnnncs tgstalllak qlveggladv vlalgfekmd
      121 rgsltskyrd ranpvekhak vlaathglap apmaaqmfga aglehmakyg strehlakia
      181 qknhrhsvhn pnsqfreeht leeilespvv fgpltklqcc ptsdgaaaav lvseavvrrl
      241 qleaqavevl siematdlps afadnsciem vgfsmtrkaa qqafrraglt pddvqvvelh
      301 dcfsanella yealglcpvg kaaelvdrgd ntyggkyvin psggliskgh plgatglaqc
      361 aelswqlrgl agprqvpgar valqhniglg gavvvalyrl gfpraal
//
LOCUS       XP_077505160             407 aa            linear   INV 25-NOV-2025
DEFINITION  sterol carrier protein 2-like [Amblyomma americanum].
ACCESSION   XP_077505160
VERSION     XP_077505160.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649034.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..407
                     /product="sterol carrier protein 2-like"
                     /calculated_mol_wt=43138
     Region          9..399
                     /region_name="PRK08256"
                     /note="lipid-transfer protein; Provisional"
                     /db_xref="CDD:181327"
     CDS             1..407
                     /gene="LOC144114962"
                     /coded_by="XM_077649034.1:254..1477"
                     /db_xref="GeneID:144114962"
ORIGIN      
        1 maaaerggrr vfvvgvgmtk fakpgdkkrd ypvlakeaat aalqdcrmey gdveqaccgy
       61 vygdstcgqr cvyelgmtgi pvynvnnncs tgstalllak qlveggladv vlalgfekmd
      121 rgsltskyrd ranpvekhak vlaathglap apmaaqmfga aglehmakyg strehlakia
      181 qknhrhsvhn pnsqfreeht leeilespvv fgpltklqcc ptsdgaaaav lvseavvrrl
      241 qleaqavevl siematdlps afadnsciem vgfsmtrkaa qqafrraglt pddvqvvelh
      301 dcfsanella yealglcpvg kaaelvdrgd ntyggkyvin psggliskgh plgatglaqc
      361 aelswqlrgl agprqvpgar valqhniglg gavvvalyrl gfpraal
//
LOCUS       XP_077505161             368 aa            linear   INV 25-NOV-2025
DEFINITION  deoxyhypusine synthase [Amblyomma americanum].
ACCESSION   XP_077505161
VERSION     XP_077505161.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649035.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..368
                     /product="deoxyhypusine synthase"
                     /calculated_mol_wt=40899
     Region          43..352
                     /region_name="DS"
                     /note="Deoxyhypusine synthase; pfam01916"
                     /db_xref="CDD:460382"
     CDS             1..368
                     /gene="Dhps"
                     /coded_by="XM_077649035.1:190..1296"
                     /db_xref="GeneID:144114963"
ORIGIN      
        1 mnaqgepaia vesvlrrsge lpvaekievk gydfndgidy arilesykcs gfqathfdra
       61 vneinrmlec ravplgndql ddretdefir rkygctiflg ytsnmasagv rdvirflveh
      121 kmvdcivtta ggveedlikc laptyigdft lkgsslrdlg inrignllap ndnyclfenw
      181 vipifnamle eqqtqgtiws pskmierfgn eindkssiyy waaknkipvf cpaltdgslg
      241 dmiyfhsyrn pglivdiaqd irrinsiavk avrsgmvivg gglvkhhicn snmmrngadf
      301 avymntaaef dgsdsgatpd eavswgkirk dahpvkiyad atlvfpllva ktfatwhfrh
      361 kqlangeq
//
LOCUS       XP_077505162             325 aa            linear   INV 25-NOV-2025
DEFINITION  pseudouridylate synthase TRUB2, mitochondrial isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505162
VERSION     XP_077505162.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649036.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..325
                     /product="pseudouridylate synthase TRUB2, mitochondrial
                     isoform X1"
                     /calculated_mol_wt=36134
     Region          21..290
                     /region_name="PseudoU_synth"
                     /note="Pseudouridine synthases catalyze the isomerization
                     of specific uridines in an RNA molecule to pseudouridines
                     (5-ribosyluracil, psi); cl00130"
                     /db_xref="CDD:469624"
     Site            order(102..105,243)
                     /site_type="active"
                     /db_xref="CDD:211324"
     CDS             1..325
                     /gene="LOC144114964"
                     /coded_by="XM_077649036.1:350..1327"
                     /db_xref="GeneID:144114964"
ORIGIN      
        1 masvslqtvt sapkafdllr gifcvykpan isceavrrti ignlcrdlne ldvrppvdfv
       61 tiegpvsggk eltvktspny adhplvvgsr yqpedikmcc ginlgkytsg vllmrvnggy
      121 hklklicdsn qlrtyqlrge fgkasdthtp lsrivdkatf rhitvakler llgsiqsayq
      181 vqafkyagvp lnsqaafema atatvrpaek sptlvynirc velnlpyftl evsciheten
      241 yllqlvheig lrlhscaicn girltryglf ntdlallrkh wtleyilrnl qdirplvsqk
      301 llepqsphlv dvndinqtgc gqpsf
//
LOCUS       XP_077505163             248 aa            linear   INV 25-NOV-2025
DEFINITION  pseudouridylate synthase TRUB2, mitochondrial isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505163
VERSION     XP_077505163.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649037.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..248
                     /product="pseudouridylate synthase TRUB2, mitochondrial
                     isoform X2"
                     /calculated_mol_wt=27328
     Region          21..>230
                     /region_name="PseudoU_synth"
                     /note="Pseudouridine synthases catalyze the isomerization
                     of specific uridines in an RNA molecule to pseudouridines
                     (5-ribosyluracil, psi); cl00130"
                     /db_xref="CDD:469624"
     Site            102..105
                     /site_type="active"
                     /db_xref="CDD:211324"
     CDS             1..248
                     /gene="LOC144114964"
                     /coded_by="XM_077649037.1:350..1096"
                     /db_xref="GeneID:144114964"
ORIGIN      
        1 masvslqtvt sapkafdllr gifcvykpan isceavrrti ignlcrdlne ldvrppvdfv
       61 tiegpvsggk eltvktspny adhplvvgsr yqpedikmcc ginlgkytsg vllmrvnggy
      121 hklklicdsn qlrtyqlrge fgkasdthtp lsrivdkatf rhitvakler llgsiqsayq
      181 vqafkyagvp lnsqaafema atatvrpaek sptlvynirc velnlpyftl aqswskqevi
      241 ktraiekk
//
LOCUS       XP_077505164             243 aa            linear   INV 25-NOV-2025
DEFINITION  pseudouridylate synthase TRUB2, mitochondrial isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505164
VERSION     XP_077505164.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649038.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..243
                     /product="pseudouridylate synthase TRUB2, mitochondrial
                     isoform X3"
                     /calculated_mol_wt=26722
     Region          21..>230
                     /region_name="PseudoU_synth"
                     /note="Pseudouridine synthases catalyze the isomerization
                     of specific uridines in an RNA molecule to pseudouridines
                     (5-ribosyluracil, psi); cl00130"
                     /db_xref="CDD:469624"
     Site            102..105
                     /site_type="active"
                     /db_xref="CDD:211324"
     CDS             1..243
                     /gene="LOC144114964"
                     /coded_by="XM_077649038.1:350..1081"
                     /db_xref="GeneID:144114964"
ORIGIN      
        1 masvslqtvt sapkafdllr gifcvykpan isceavrrti ignlcrdlne ldvrppvdfv
       61 tiegpvsggk eltvktspny adhplvvgsr yqpedikmcc ginlgkytsg vllmrvnggy
      121 hklklicdsn qlrtyqlrge fgkasdthtp lsrivdkatf rhitvakler llgsiqsayq
      181 vqafkyagvp lnsqaafema atatvrpaek sptlvynirc velnlpyftl gervylllsk
      241 lhp
//
LOCUS       XP_077505165            1342 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114967 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505165
VERSION     XP_077505165.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649039.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1342
                     /product="uncharacterized protein LOC144114967 isoform X1"
                     /calculated_mol_wt=140581
     Region          2..72
                     /region_name="Ubl_BAG6"
                     /note="ubiquitin-like (Ubl) domain found in
                     BCL2-associated athanogene 6 (BAG6) and similar proteins;
                     cd01809"
                     /db_xref="CDD:340507"
     Site            order(7..10,43,45,47..50,68..72)
                     /site_type="other"
                     /note="ZF interaction site [polypeptide binding]"
                     /db_xref="CDD:340507"
     Region          367..474
                     /region_name="BAG6"
                     /note="BCL2-associated athanogene 6; pfam12057"
                     /db_xref="CDD:463449"
     CDS             1..1342
                     /gene="LOC144114967"
                     /coded_by="XM_077649039.1:252..4280"
                     /db_xref="GeneID:144114967"
ORIGIN      
        1 mlevtvktld sqnrnysvpd ditvksfkek iassvnitad kqrlifcgrv lqddkklsdy
       61 dvngkvihlv qrpppqpssg gssssgatgt gstsggggsr snasgglhhp regggfllga
      121 ftipqdmidp aqvqqivqdv vsgmgeigrn atvmsrtsed gssvdvhinl gqvpmqteaq
      181 lrlsqaqtml qkaarvivql enqdqqiasa pdvegmdlge rvevdvqmvd sngndtapps
      241 tsggndidaa asrplglass tgrfgegivn aaraaaaaaa vvaarvtags vfgssaatst
      301 enpattsaav gsqrgspsqp vpdggpapvq psapttaaas gttqqgpaqq ntsgrplrdy
      361 gapasslapi lgeilhlqgr lapylqryqe lvqdernqtm tdteahrlgn gvccalhhls
      421 halhavsdlh myvgsrpriv rarfvgpsqg gsifhtnmpf qgqinvstaq spssesiatt
      481 rvtsgttsts atsttaqgap tssstapssg gpfsttgpal ltaqspvvfm elgpgsitid
      541 sistavindl pspldrivdq pegttpsats aaednpasqa gsgssqaraq ttgaatvtts
      601 sgttgtrasp gpfrglspls fplsstlgga lgssfdpclp cnsvwahsqa astypmgsns
      661 qssratqqgs tqapqsanqh ddqlhqmlng lmtallhqph lvlqrqhmhm gqgsssessg
      721 srpratlagm mpgrsrhmsm rqtplvyrgn gpttattgtn tvpaelgqnr rlggsgslws
      781 gsssgsaqqg pssatavqqq qqaekqpasa eardkearsg ptaastsase rpvprsrrtl
      841 essasrvlhp pgvlvptysl qspltnvmev vlndyswern lsdaasllev fvrenvvspn
      901 lleedfvtsv laslsrelta edlasllagn qpgrldrlqg alqlavmqhl tpgsladlpc
      961 lvvpardall arwaapliqd mqakgnvkpd idveatingf lserlhavll hilgprkenf
     1021 gqellslikv avsdlvalal scfedgtesl naifrarmdp illglsrnls cktqvffcsw
     1081 vgafwkkvsd glsssqaata aiaakylvqh naqprassgs lsnqngatpp smevdlevte
     1141 qssaergahg lnngatanva sesrpappsm deqplpvilg seswhasvps pvpkatqltq
     1201 lrarraiagv pceeerhsge wvpiitrdiq rqrrqaaqpp fsdayfcgmp skrrkimngs
     1261 svgtesagsl tealqravqr agvraprsgm eaflaearqn palqtahklq lrqavrerls
     1321 sdpdfrperf pnahkfyysa dp
//
LOCUS       XP_077505166            1342 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114967 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505166
VERSION     XP_077505166.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649040.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1342
                     /product="uncharacterized protein LOC144114967 isoform X1"
                     /calculated_mol_wt=140581
     Region          2..72
                     /region_name="Ubl_BAG6"
                     /note="ubiquitin-like (Ubl) domain found in
                     BCL2-associated athanogene 6 (BAG6) and similar proteins;
                     cd01809"
                     /db_xref="CDD:340507"
     Site            order(7..10,43,45,47..50,68..72)
                     /site_type="other"
                     /note="ZF interaction site [polypeptide binding]"
                     /db_xref="CDD:340507"
     Region          367..474
                     /region_name="BAG6"
                     /note="BCL2-associated athanogene 6; pfam12057"
                     /db_xref="CDD:463449"
     CDS             1..1342
                     /gene="LOC144114967"
                     /coded_by="XM_077649040.1:44..4072"
                     /db_xref="GeneID:144114967"
ORIGIN      
        1 mlevtvktld sqnrnysvpd ditvksfkek iassvnitad kqrlifcgrv lqddkklsdy
       61 dvngkvihlv qrpppqpssg gssssgatgt gstsggggsr snasgglhhp regggfllga
      121 ftipqdmidp aqvqqivqdv vsgmgeigrn atvmsrtsed gssvdvhinl gqvpmqteaq
      181 lrlsqaqtml qkaarvivql enqdqqiasa pdvegmdlge rvevdvqmvd sngndtapps
      241 tsggndidaa asrplglass tgrfgegivn aaraaaaaaa vvaarvtags vfgssaatst
      301 enpattsaav gsqrgspsqp vpdggpapvq psapttaaas gttqqgpaqq ntsgrplrdy
      361 gapasslapi lgeilhlqgr lapylqryqe lvqdernqtm tdteahrlgn gvccalhhls
      421 halhavsdlh myvgsrpriv rarfvgpsqg gsifhtnmpf qgqinvstaq spssesiatt
      481 rvtsgttsts atsttaqgap tssstapssg gpfsttgpal ltaqspvvfm elgpgsitid
      541 sistavindl pspldrivdq pegttpsats aaednpasqa gsgssqaraq ttgaatvtts
      601 sgttgtrasp gpfrglspls fplsstlgga lgssfdpclp cnsvwahsqa astypmgsns
      661 qssratqqgs tqapqsanqh ddqlhqmlng lmtallhqph lvlqrqhmhm gqgsssessg
      721 srpratlagm mpgrsrhmsm rqtplvyrgn gpttattgtn tvpaelgqnr rlggsgslws
      781 gsssgsaqqg pssatavqqq qqaekqpasa eardkearsg ptaastsase rpvprsrrtl
      841 essasrvlhp pgvlvptysl qspltnvmev vlndyswern lsdaasllev fvrenvvspn
      901 lleedfvtsv laslsrelta edlasllagn qpgrldrlqg alqlavmqhl tpgsladlpc
      961 lvvpardall arwaapliqd mqakgnvkpd idveatingf lserlhavll hilgprkenf
     1021 gqellslikv avsdlvalal scfedgtesl naifrarmdp illglsrnls cktqvffcsw
     1081 vgafwkkvsd glsssqaata aiaakylvqh naqprassgs lsnqngatpp smevdlevte
     1141 qssaergahg lnngatanva sesrpappsm deqplpvilg seswhasvps pvpkatqltq
     1201 lrarraiagv pceeerhsge wvpiitrdiq rqrrqaaqpp fsdayfcgmp skrrkimngs
     1261 svgtesagsl tealqravqr agvraprsgm eaflaearqn palqtahklq lrqavrerls
     1321 sdpdfrperf pnahkfyysa dp
//
LOCUS       XP_077505167            1339 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114967 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505167
VERSION     XP_077505167.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649041.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1339
                     /product="uncharacterized protein LOC144114967 isoform X2"
                     /calculated_mol_wt=140232
     Region          2..72
                     /region_name="Ubl_BAG6"
                     /note="ubiquitin-like (Ubl) domain found in
                     BCL2-associated athanogene 6 (BAG6) and similar proteins;
                     cd01809"
                     /db_xref="CDD:340507"
     Site            order(7..10,43,45,47..50,68..72)
                     /site_type="other"
                     /note="ZF interaction site [polypeptide binding]"
                     /db_xref="CDD:340507"
     Region          367..474
                     /region_name="BAG6"
                     /note="BCL2-associated athanogene 6; pfam12057"
                     /db_xref="CDD:463449"
     CDS             1..1339
                     /gene="LOC144114967"
                     /coded_by="XM_077649041.1:256..4275"
                     /db_xref="GeneID:144114967"
ORIGIN      
        1 mlevtvktld sqnrnysvpd ditvksfkek iassvnitad kqrlifcgrv lqddkklsdy
       61 dvngkvihlv qrpppqpssg gssssgatgt gstsggggsr snasgglhhp regggfllga
      121 ftipqdmidp aqvqqivqdv vsgmgeigrn atvmsrtsed gssvdvhinl gqvpmqteaq
      181 lrlsqaqtml qkaarvivql enqdqqiasa pdvegmdlge rvevdvqmvd sngndtapps
      241 tsggndidaa asrplglass tgrfgegivn aaraaaaaaa vvaarvtags vfgssaatst
      301 enpattsaav gsqrgspsqp vpdggpapvq psapttaaas gttqqgpaqq ntsgrplrdy
      361 gapasslapi lgeilhlqgr lapylqryqe lvqdernqtm tdteahrlgn gvccalhhls
      421 halhavsdlh myvgsrpriv rarfvgpsqg gsifhtnmpf qgqinvstaq spssesiatt
      481 rvtsgttsts atsttaqgap tssstapssg gpfsttgpal ltaqspvvfm elgpgsitid
      541 sistavindl pspldrivdq pegttpsats aaednpasqa gsgssqaraq ttgaatvtts
      601 sgttgtrasp gpfrglspls fplsstlgga lgssfdpclp cnsvwahsqa astypmgsns
      661 qssratqqgs tqapqsanqh ddqlhqmlng lmtallhqph lvlqrqhmhm gqgsssessg
      721 srpratlagm mpgrsrhmsm rqtplvyrgn gpttattgtn tvpaelgqnr rlggsgslws
      781 gsssgsaqqg pssatavqqq qqaekqpasa eardkearsg ptaastsase rpvprsrrtl
      841 essasrppgv lvptyslqsp ltnvmevvln dyswernlsd aasllevfvr envvspnlle
      901 edfvtsvlas lsreltaedl asllagnqpg rldrlqgalq lavmqhltpg sladlpclvv
      961 pardallarw aapliqdmqa kgnvkpdidv eatingflse rlhavllhil gprkenfgqe
     1021 llslikvavs dlvalalscf edgteslnai frarmdpill glsrnlsckt qvffcswvga
     1081 fwkkvsdgls ssqaataaia akylvqhnaq prassgslsn qngatppsme vdlevteqss
     1141 aergahglnn gatanvases rpappsmdeq plpvilgses whasvpspvp katqltqlra
     1201 rraiagvpce eerhsgewvp iitrdiqrqr rqaaqppfsd ayfcgmpskr rkimngssvg
     1261 tesagsltea lqravqragv raprsgmeaf laearqnpal qtahklqlrq avrerlssdp
     1321 dfrperfpna hkfyysadp
//
LOCUS       XP_077505168            1313 aa            linear   INV 25-NOV-2025
DEFINITION  large proline-rich protein BAG6 isoform X3 [Amblyomma americanum].
ACCESSION   XP_077505168
VERSION     XP_077505168.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649042.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1313
                     /product="large proline-rich protein BAG6 isoform X3"
                     /calculated_mol_wt=137428
     Region          2..72
                     /region_name="Ubl_BAG6"
                     /note="ubiquitin-like (Ubl) domain found in
                     BCL2-associated athanogene 6 (BAG6) and similar proteins;
                     cd01809"
                     /db_xref="CDD:340507"
     Site            order(7..10,43,45,47..50,68..72)
                     /site_type="other"
                     /note="ZF interaction site [polypeptide binding]"
                     /db_xref="CDD:340507"
     Region          367..474
                     /region_name="BAG6"
                     /note="BCL2-associated athanogene 6; pfam12057"
                     /db_xref="CDD:463449"
     CDS             1..1313
                     /gene="LOC144114967"
                     /coded_by="XM_077649042.1:252..4193"
                     /db_xref="GeneID:144114967"
ORIGIN      
        1 mlevtvktld sqnrnysvpd ditvksfkek iassvnitad kqrlifcgrv lqddkklsdy
       61 dvngkvihlv qrpppqpssg gssssgatgt gstsggggsr snasgglhhp regggfllga
      121 ftipqdmidp aqvqqivqdv vsgmgeigrn atvmsrtsed gssvdvhinl gqvpmqteaq
      181 lrlsqaqtml qkaarvivql enqdqqiasa pdvegmdlge rvevdvqmvd sngndtapps
      241 tsggndidaa asrplglass tgrfgegivn aaraaaaaaa vvaarvtags vfgssaatst
      301 enpattsaav gsqrgspsqp vpdggpapvq psapttaaas gttqqgpaqq ntsgrplrdy
      361 gapasslapi lgeilhlqgr lapylqryqe lvqdernqtm tdteahrlgn gvccalhhls
      421 halhavsdlh myvgsrpriv rarfvgpsqg gsifhtnmpf qgqinvstaq spssesiatt
      481 rvtsgttsts atsttaqgap tssstapssg gpfsttgpal ltaqspvvfm elgpgsitid
      541 sistavindl pspldrivdq pegttpsats aaednpasqa gsgssqaraq ttgaatvtts
      601 sgttgtrasp gpfrglspls fplsstlgga lgssfdpclp cnsvwahsqa astypmgsns
      661 qssratqqgs tqapqsanqh ddqlhqmlng lmtallhqph lvlqrqhmhm gqgsssessg
      721 srpratlagm mpgrsrhmsm rqtplvyrgn gpttattgtn tvpaelgqnr rlggsgslws
      781 gsssgsaqqg pssatavqqq qqaekqpasa eardkearsg ptaastsase rpvprsrrtl
      841 essasrvlhp pgvlvptysl qspltnvmev vlndyswern lsdaasllev fvrenvvspn
      901 lleedfvtsv laslsrelta edlasllagn qpgrldrlqg alqlavmqhl tpgsladlpc
      961 lvvpardall arwaapliqd mqakgnvkpd idveatingf lserlhavll hilgprkenf
     1021 gqellslikv avsdlvalal scfedgtesl naifrarmdp illglsrnls cktqvffcsw
     1081 vgafwkkvsd glsssqaata aiaakylvqh naqprassgs lsnqngatpp smevdlevte
     1141 qssaergahg lnngatanva sesrpappsm deqplpvilg seswhasvps ewvpiitrdi
     1201 qrqrrqaaqp pfsdayfcgm pskrrkimng ssvgtesags ltealqravq ragvraprsg
     1261 meaflaearq npalqtahkl qlrqavrerl ssdpdfrper fpnahkfyys adp
//
LOCUS       XP_077505169            1310 aa            linear   INV 25-NOV-2025
DEFINITION  large proline-rich protein BAG6 isoform X4 [Amblyomma americanum].
ACCESSION   XP_077505169
VERSION     XP_077505169.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649043.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1310
                     /product="large proline-rich protein BAG6 isoform X4"
                     /calculated_mol_wt=137078
     Region          2..72
                     /region_name="Ubl_BAG6"
                     /note="ubiquitin-like (Ubl) domain found in
                     BCL2-associated athanogene 6 (BAG6) and similar proteins;
                     cd01809"
                     /db_xref="CDD:340507"
     Site            order(7..10,43,45,47..50,68..72)
                     /site_type="other"
                     /note="ZF interaction site [polypeptide binding]"
                     /db_xref="CDD:340507"
     Region          367..474
                     /region_name="BAG6"
                     /note="BCL2-associated athanogene 6; pfam12057"
                     /db_xref="CDD:463449"
     CDS             1..1310
                     /gene="LOC144114967"
                     /coded_by="XM_077649043.1:256..4188"
                     /db_xref="GeneID:144114967"
ORIGIN      
        1 mlevtvktld sqnrnysvpd ditvksfkek iassvnitad kqrlifcgrv lqddkklsdy
       61 dvngkvihlv qrpppqpssg gssssgatgt gstsggggsr snasgglhhp regggfllga
      121 ftipqdmidp aqvqqivqdv vsgmgeigrn atvmsrtsed gssvdvhinl gqvpmqteaq
      181 lrlsqaqtml qkaarvivql enqdqqiasa pdvegmdlge rvevdvqmvd sngndtapps
      241 tsggndidaa asrplglass tgrfgegivn aaraaaaaaa vvaarvtags vfgssaatst
      301 enpattsaav gsqrgspsqp vpdggpapvq psapttaaas gttqqgpaqq ntsgrplrdy
      361 gapasslapi lgeilhlqgr lapylqryqe lvqdernqtm tdteahrlgn gvccalhhls
      421 halhavsdlh myvgsrpriv rarfvgpsqg gsifhtnmpf qgqinvstaq spssesiatt
      481 rvtsgttsts atsttaqgap tssstapssg gpfsttgpal ltaqspvvfm elgpgsitid
      541 sistavindl pspldrivdq pegttpsats aaednpasqa gsgssqaraq ttgaatvtts
      601 sgttgtrasp gpfrglspls fplsstlgga lgssfdpclp cnsvwahsqa astypmgsns
      661 qssratqqgs tqapqsanqh ddqlhqmlng lmtallhqph lvlqrqhmhm gqgsssessg
      721 srpratlagm mpgrsrhmsm rqtplvyrgn gpttattgtn tvpaelgqnr rlggsgslws
      781 gsssgsaqqg pssatavqqq qqaekqpasa eardkearsg ptaastsase rpvprsrrtl
      841 essasrppgv lvptyslqsp ltnvmevvln dyswernlsd aasllevfvr envvspnlle
      901 edfvtsvlas lsreltaedl asllagnqpg rldrlqgalq lavmqhltpg sladlpclvv
      961 pardallarw aapliqdmqa kgnvkpdidv eatingflse rlhavllhil gprkenfgqe
     1021 llslikvavs dlvalalscf edgteslnai frarmdpill glsrnlsckt qvffcswvga
     1081 fwkkvsdgls ssqaataaia akylvqhnaq prassgslsn qngatppsme vdlevteqss
     1141 aergahglnn gatanvases rpappsmdeq plpvilgses whasvpsewv piitrdiqrq
     1201 rrqaaqppfs dayfcgmpsk rrkimngssv gtesagslte alqravqrag vraprsgmea
     1261 flaearqnpa lqtahklqlr qavrerlssd pdfrperfpn ahkfyysadp
//
LOCUS       XP_077505170            1310 aa            linear   INV 25-NOV-2025
DEFINITION  large proline-rich protein BAG6 isoform X4 [Amblyomma americanum].
ACCESSION   XP_077505170
VERSION     XP_077505170.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649044.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1310
                     /product="large proline-rich protein BAG6 isoform X4"
                     /calculated_mol_wt=137078
     Region          2..72
                     /region_name="Ubl_BAG6"
                     /note="ubiquitin-like (Ubl) domain found in
                     BCL2-associated athanogene 6 (BAG6) and similar proteins;
                     cd01809"
                     /db_xref="CDD:340507"
     Site            order(7..10,43,45,47..50,68..72)
                     /site_type="other"
                     /note="ZF interaction site [polypeptide binding]"
                     /db_xref="CDD:340507"
     Region          367..474
                     /region_name="BAG6"
                     /note="BCL2-associated athanogene 6; pfam12057"
                     /db_xref="CDD:463449"
     CDS             1..1310
                     /gene="LOC144114967"
                     /coded_by="XM_077649044.1:41..3973"
                     /db_xref="GeneID:144114967"
ORIGIN      
        1 mlevtvktld sqnrnysvpd ditvksfkek iassvnitad kqrlifcgrv lqddkklsdy
       61 dvngkvihlv qrpppqpssg gssssgatgt gstsggggsr snasgglhhp regggfllga
      121 ftipqdmidp aqvqqivqdv vsgmgeigrn atvmsrtsed gssvdvhinl gqvpmqteaq
      181 lrlsqaqtml qkaarvivql enqdqqiasa pdvegmdlge rvevdvqmvd sngndtapps
      241 tsggndidaa asrplglass tgrfgegivn aaraaaaaaa vvaarvtags vfgssaatst
      301 enpattsaav gsqrgspsqp vpdggpapvq psapttaaas gttqqgpaqq ntsgrplrdy
      361 gapasslapi lgeilhlqgr lapylqryqe lvqdernqtm tdteahrlgn gvccalhhls
      421 halhavsdlh myvgsrpriv rarfvgpsqg gsifhtnmpf qgqinvstaq spssesiatt
      481 rvtsgttsts atsttaqgap tssstapssg gpfsttgpal ltaqspvvfm elgpgsitid
      541 sistavindl pspldrivdq pegttpsats aaednpasqa gsgssqaraq ttgaatvtts
      601 sgttgtrasp gpfrglspls fplsstlgga lgssfdpclp cnsvwahsqa astypmgsns
      661 qssratqqgs tqapqsanqh ddqlhqmlng lmtallhqph lvlqrqhmhm gqgsssessg
      721 srpratlagm mpgrsrhmsm rqtplvyrgn gpttattgtn tvpaelgqnr rlggsgslws
      781 gsssgsaqqg pssatavqqq qqaekqpasa eardkearsg ptaastsase rpvprsrrtl
      841 essasrppgv lvptyslqsp ltnvmevvln dyswernlsd aasllevfvr envvspnlle
      901 edfvtsvlas lsreltaedl asllagnqpg rldrlqgalq lavmqhltpg sladlpclvv
      961 pardallarw aapliqdmqa kgnvkpdidv eatingflse rlhavllhil gprkenfgqe
     1021 llslikvavs dlvalalscf edgteslnai frarmdpill glsrnlsckt qvffcswvga
     1081 fwkkvsdgls ssqaataaia akylvqhnaq prassgslsn qngatppsme vdlevteqss
     1141 aergahglnn gatanvases rpappsmdeq plpvilgses whasvpsewv piitrdiqrq
     1201 rrqaaqppfs dayfcgmpsk rrkimngssv gtesagslte alqravqrag vraprsgmea
     1261 flaearqnpa lqtahklqlr qavrerlssd pdfrperfpn ahkfyysadp
//
LOCUS       XP_077505171             643 aa            linear   INV 25-NOV-2025
DEFINITION  protein SCAI isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505171
VERSION     XP_077505171.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649045.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..643
                     /product="protein SCAI isoform X1"
                     /calculated_mol_wt=74026
     Region          53..566
                     /region_name="SCAI"
                     /note="Protein SCAI; pfam12070"
                     /db_xref="CDD:463455"
     CDS             1..643
                     /gene="LOC144114968"
                     /coded_by="XM_077649045.1:228..2159"
                     /db_xref="GeneID:144114968"
ORIGIN      
        1 mqkgsasyyd rldpvkrpas ggpedlgqha prrmaimsei eeqerkivne fchllekskq
       61 lfnglrdlpq yghkqwqayf grtfdvytkl wkfqqqhrqi ldikyglkrw qigeiaskig
      121 qlyyhyylrt setnylneaf sfyaairarg yyskaskees lpyshrsdlm vkklryyarf
      181 ivvclllkkm klvrdlvrel akqiddytat yepedqlews lvlgeiksfi dadnlvnvvd
      241 ldsssivlsh rlsplntppm ekvpsshlsl qeiliigncc dqmadppqvk fseltldmfr
      301 mlqtlerepq edttqlydas papgrvpfpe nggtgdsrpg krenphkyll ykptfsqlfv
      361 flasgfkelp pngvlllyvs adgsfpnvkh peevgydygg vttnskrepd hsnkrnvqlk
      421 dmhclypgdl ypytrkplfl imdsdnshvf qhmprffgqp lvalmspedv ppafhdqqhk
      481 gnlltlflhs pltalcfvch vvdvpvslwd kalghlnrfm aeasrvlirs rsidpaylqf
      541 ygddflrllm lrfifchvvl klhrmfkiky svgdpenlyq rmpqaamgtp pyqslpgsny
      601 lprshppipe sevldhpalq rtilelaevl dvrslfseid ead
//
LOCUS       XP_077505172             637 aa            linear   INV 25-NOV-2025
DEFINITION  protein SCAI isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505172
VERSION     XP_077505172.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649046.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..637
                     /product="protein SCAI isoform X2"
                     /calculated_mol_wt=73386
     Region          53..560
                     /region_name="SCAI"
                     /note="Protein SCAI; pfam12070"
                     /db_xref="CDD:463455"
     CDS             1..637
                     /gene="LOC144114968"
                     /coded_by="XM_077649046.1:191..2104"
                     /db_xref="GeneID:144114968"
ORIGIN      
        1 mqkgsasyyd rldpvkrpas ggpedlgqha prrmaimsei eeqerkivne fchllekskq
       61 lfnglrdlpq yghkqwqayf grtfdvytkl wkfqqqhrqi ldikyglkrw qigeiaskig
      121 qlyyhyylrt setnylneaf sfyaairarg yyskaskees lpyshrsdlm vkklryyarf
      181 ivvclllkkm klvrdlvrel akqiddytat yepedqlews lvlgeiksfi dadnlvnvvd
      241 ldsssivlsh rlsplntppm ekvpsshlsl qeiliigncc dqvkfseltl dmfrmlqtle
      301 repqedttql ydaspapgrv pfpenggtgd srpgkrenph kyllykptfs qlfvflasgf
      361 kelppngvll lyvsadgsfp nvkhpeevgy dyggvttnsk repdhsnkrn vqlkdmhcly
      421 pgdlypytrk plflimdsdn shvfqhmprf fgqplvalms pedvppafhd qqhkgnlltl
      481 flhspltalc fvchvvdvpv slwdkalghl nrfmaeasrv lirsrsidpa ylqfygddfl
      541 rllmlrfifc hvvlklhrmf kikysvgdpe nlyqrmpqaa mgtppyqslp gsnylprshp
      601 pipesevldh palqrtilel aevldvrslf seidead
//
LOCUS       XP_077505173             614 aa            linear   INV 25-NOV-2025
DEFINITION  protein SCAI isoform X3 [Amblyomma americanum].
ACCESSION   XP_077505173
VERSION     XP_077505173.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649047.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..614
                     /product="protein SCAI isoform X3"
                     /calculated_mol_wt=70791
     Region          53..566
                     /region_name="SCAI"
                     /note="Protein SCAI; pfam12070"
                     /db_xref="CDD:463455"
     CDS             1..614
                     /gene="LOC144114968"
                     /coded_by="XM_077649047.1:228..2072"
                     /db_xref="GeneID:144114968"
ORIGIN      
        1 mqkgsasyyd rldpvkrpas ggpedlgqha prrmaimsei eeqerkivne fchllekskq
       61 lfnglrdlpq yghkqwqayf grtfdvytkl wkfqqqhrqi ldikyglkrw qigeiaskig
      121 qlyyhyylrt setnylneaf sfyaairarg yyskaskees lpyshrsdlm vkklryyarf
      181 ivvclllkkm klvrdlvrel akqiddytat yepedqlews lvlgeiksfi dadnlvnvvd
      241 ldsssivlsh rlsplntppm ekvpsshlsl qeiliigncc dqmadppqvk fseltldmfr
      301 mlqtlerepq edttqlydas papgrvpfpe nggtgdsrpg krenphkyll ykptfsqlfv
      361 flasgfkelp pngvlllyvs adgsfpnvkh peevgydygg vttnskrepd hsnkrnvqlk
      421 dmhclypgdl ypytrkplfl imdsdnshvf qhmprffgqp lvalmspedv ppafhdqqhk
      481 gnlltlflhs pltalcfvch vvdvpvslwd kalghlnrfm aeasrvlirs rsidpaylqf
      541 ygddflrllm lrfifchvvl klhrmfkgsn ylprshppip esevldhpal qrtilelaev
      601 ldvrslfsei dead
//
LOCUS       XP_077505174             608 aa            linear   INV 25-NOV-2025
DEFINITION  protein SCAI isoform X4 [Amblyomma americanum].
ACCESSION   XP_077505174
VERSION     XP_077505174.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649048.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..608
                     /product="protein SCAI isoform X4"
                     /calculated_mol_wt=70152
     Region          53..560
                     /region_name="SCAI"
                     /note="Protein SCAI; pfam12070"
                     /db_xref="CDD:463455"
     CDS             1..608
                     /gene="LOC144114968"
                     /coded_by="XM_077649048.1:191..2017"
                     /db_xref="GeneID:144114968"
ORIGIN      
        1 mqkgsasyyd rldpvkrpas ggpedlgqha prrmaimsei eeqerkivne fchllekskq
       61 lfnglrdlpq yghkqwqayf grtfdvytkl wkfqqqhrqi ldikyglkrw qigeiaskig
      121 qlyyhyylrt setnylneaf sfyaairarg yyskaskees lpyshrsdlm vkklryyarf
      181 ivvclllkkm klvrdlvrel akqiddytat yepedqlews lvlgeiksfi dadnlvnvvd
      241 ldsssivlsh rlsplntppm ekvpsshlsl qeiliigncc dqvkfseltl dmfrmlqtle
      301 repqedttql ydaspapgrv pfpenggtgd srpgkrenph kyllykptfs qlfvflasgf
      361 kelppngvll lyvsadgsfp nvkhpeevgy dyggvttnsk repdhsnkrn vqlkdmhcly
      421 pgdlypytrk plflimdsdn shvfqhmprf fgqplvalms pedvppafhd qqhkgnlltl
      481 flhspltalc fvchvvdvpv slwdkalghl nrfmaeasrv lirsrsidpa ylqfygddfl
      541 rllmlrfifc hvvlklhrmf kgsnylprsh ppipesevld hpalqrtile laevldvrsl
      601 fseidead
//
LOCUS       XP_077505175             232 aa            linear   INV 25-NOV-2025
DEFINITION  proteasome beta6 subunit [Amblyomma americanum].
ACCESSION   XP_077505175
VERSION     XP_077505175.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649049.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..232
                     /product="proteasome beta6 subunit"
                     /calculated_mol_wt=25912
     Region          21..232
                     /region_name="proteasome_beta_type_1"
                     /note="proteasome beta type-1 subunit. The 20S proteasome,
                     multisubunit proteolytic complex, is the central enzyme of
                     nonlysosomal protein degradation in both the cytosol and
                     nucleus. It is composed of 28 subunits arranged as four
                     homoheptameric rings that...; cd03757"
                     /db_xref="CDD:239726"
     Site            order(29,45,47,61,159,205,208..209)
                     /site_type="active"
                     /db_xref="CDD:239726"
     Site            order(47,50,52..57,77,79,94,106,109..110,112..113,116,119,
                     144,146,148..151,154,162..163,165..166,169..171,196,
                     203..206,209..210)
                     /site_type="other"
                     /note="beta subunit interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:239726"
     CDS             1..232
                     /gene="Prosbeta6"
                     /coded_by="XM_077649049.1:131..829"
                     /db_xref="GeneID:144114969"
ORIGIN      
        1 mdmlaeqtal syyggpkqay fspysenggs ivaiagddfa viasdsrlst gyhiytreqs
       61 klfklsnqcv lgstgcwcdi ltftrflear mkmylhehhr lmstpavaql vvtmlynkrf
      121 fpyyisnila gldeegkgci ycydpvghce rvqyraggss sallqplldn qigfknmenv
      181 elvpptrera vsiikdvfis aaerdiytgd svvinvidkn gikeemlalr rd
//
LOCUS       XP_077505176             147 aa            linear   INV 25-NOV-2025
DEFINITION  multiprotein bridging factor 1 [Amblyomma americanum].
ACCESSION   XP_077505176
VERSION     XP_077505176.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649050.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..147
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..147
                     /product="multiprotein bridging factor 1"
                     /calculated_mol_wt=16462
     Region          5..71
                     /region_name="MBF1"
                     /note="Multiprotein bridging factor 1; pfam08523"
                     /db_xref="CDD:462508"
     Region          76..132
                     /region_name="aMBF1"
                     /note="Archaeal ribosome-binding protein aMBF1, putative
                     translation factor, contains Zn-ribbon and HTH domains
                     [Translation, ribosomal structure and biogenesis];
                     COG1813"
                     /db_xref="CDD:441418"
     CDS             1..147
                     /gene="mbf1"
                     /coded_by="XM_077649050.1:68..511"
                     /db_xref="GeneID:144114971"
ORIGIN      
        1 mseadwdtvt ylrkkpmtas qlrsrqvina aqrqgvpiet tkkfnaatnk ktvttlntak
       61 ldreteelhh drigldvgrl iqqgrqaknm tqkelatkin ekpqvindye agraipnqqv
      121 lskiekvigm klrgkekglp lgsdkkk
//
LOCUS       XP_077505177             147 aa            linear   INV 25-NOV-2025
DEFINITION  multiprotein bridging factor 1 [Amblyomma americanum].
ACCESSION   XP_077505177
VERSION     XP_077505177.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649051.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..147
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..147
                     /product="multiprotein bridging factor 1"
                     /calculated_mol_wt=16462
     Region          5..71
                     /region_name="MBF1"
                     /note="Multiprotein bridging factor 1; pfam08523"
                     /db_xref="CDD:462508"
     Region          76..132
                     /region_name="aMBF1"
                     /note="Archaeal ribosome-binding protein aMBF1, putative
                     translation factor, contains Zn-ribbon and HTH domains
                     [Translation, ribosomal structure and biogenesis];
                     COG1813"
                     /db_xref="CDD:441418"
     CDS             1..147
                     /gene="mbf1"
                     /coded_by="XM_077649051.1:156..599"
                     /db_xref="GeneID:144114971"
ORIGIN      
        1 mseadwdtvt ylrkkpmtas qlrsrqvina aqrqgvpiet tkkfnaatnk ktvttlntak
       61 ldreteelhh drigldvgrl iqqgrqaknm tqkelatkin ekpqvindye agraipnqqv
      121 lskiekvigm klrgkekglp lgsdkkk
//
LOCUS       XP_077505178             191 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114972 [Amblyomma americanum].
ACCESSION   XP_077505178
VERSION     XP_077505178.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649052.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..191
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..191
                     /product="uncharacterized protein LOC144114972"
                     /calculated_mol_wt=21186
     Region          110..174
                     /region_name="zf-DNL"
                     /note="DNL zinc finger; pfam05180"
                     /db_xref="CDD:461571"
     CDS             1..191
                     /gene="LOC144114972"
                     /coded_by="XM_077649052.1:163..738"
                     /db_xref="GeneID:144114972"
ORIGIN      
        1 msrsmskmll rslllclrar ngtwkhcerc yagysgaase ghhhklrrsl flganceqiq
       61 cyrttrvvcv ktlstsssvk ccpqtlcgsa mpetddkvrv pvaklqgrml lsflcklcst
      121 rvtkliskvs yekgivivkc hgcskhhlia dnlgwfpdld gkrnieeila skgeavrkal
      181 lkdeilevte s
//
LOCUS       XP_077505179             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114973 [Amblyomma americanum].
ACCESSION   XP_077505179
VERSION     XP_077505179.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649053.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144114973"
                     /calculated_mol_wt=14227
     CDS             1..133
                     /gene="LOC144114973"
                     /coded_by="XM_077649053.1:1..402"
                     /db_xref="GeneID:144114973"
ORIGIN      
        1 mpfgafkgfg rqllatcdsd aswtrihshi sftvaastsp naisvvpsse pisterisss
       61 vssihyqddr saapgsvtkt tklavareqk graivervqq ptshdaefsg prhlfpvpgy
      121 wlkivptslh lgl
//
LOCUS       XP_077505181             143 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114974 [Amblyomma americanum].
ACCESSION   XP_077505181
VERSION     XP_077505181.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649055.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..143
                     /product="uncharacterized protein LOC144114974"
                     /calculated_mol_wt=15235
     CDS             1..143
                     /gene="LOC144114974"
                     /coded_by="XM_077649055.1:115..546"
                     /db_xref="GeneID:144114974"
ORIGIN      
        1 metsgvipsv sakqmsppgs heaatasgng acseadqldd igsyemlieq fvrdledqsm
       61 reealpdssk kqaatpsshp sagwsttfer tpehrcfpat sarwssrraa cspatapgst
      121 gahrcwvwqn agiknrswik gvl
//
LOCUS       XP_077505182             106 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114975 [Amblyomma americanum].
ACCESSION   XP_077505182
VERSION     XP_077505182.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649056.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..106
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..106
                     /product="uncharacterized protein LOC144114975"
                     /calculated_mol_wt=12073
     CDS             1..106
                     /gene="LOC144114975"
                     /coded_by="XM_077649056.1:141..461"
                     /db_xref="GeneID:144114975"
ORIGIN      
        1 marvfegntk vtssvgpmrs tqqerkmahr lvhrrcswln tsadetvdnp tflkrtrecc
       61 hgfeqvlksc iskfadrklt msttkpfaiv atrwrlqnmq gsirai
//
LOCUS       XP_077505183             175 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114977 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505183
VERSION     XP_077505183.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649057.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..175
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..175
                     /product="uncharacterized protein LOC144114977 isoform X1"
                     /calculated_mol_wt=19998
     Region          78..>114
                     /region_name="DivIVA"
                     /note="Cell division septum initiation protein DivIVA,
                     interacts with FtsZ and MinD [Cell cycle control, cell
                     division, chromosome partitioning]; COG3599"
                     /db_xref="CDD:442818"
     CDS             1..175
                     /gene="LOC144114977"
                     /coded_by="XM_077649057.1:1149..1676"
                     /db_xref="GeneID:144114977"
ORIGIN      
        1 mvsdsapraq glqyrcrtrh rafvfsdses reemdeyilg prhrgvvvhp pkrptclaqd
       61 kvrnvkglwm larqattdel ldsiaecgge eeieklrken eelrqlnmkl qnqllrflsa
      121 dalpepapsv amaaasahlh lwhqhlqqip lwprhsrllp ihpwpplghh qapfl
//
LOCUS       XP_077505184             175 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114977 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505184
VERSION     XP_077505184.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649058.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..175
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..175
                     /product="uncharacterized protein LOC144114977 isoform X1"
                     /calculated_mol_wt=19998
     Region          78..>114
                     /region_name="DivIVA"
                     /note="Cell division septum initiation protein DivIVA,
                     interacts with FtsZ and MinD [Cell cycle control, cell
                     division, chromosome partitioning]; COG3599"
                     /db_xref="CDD:442818"
     CDS             1..175
                     /gene="LOC144114977"
                     /coded_by="XM_077649058.1:1149..1676"
                     /db_xref="GeneID:144114977"
ORIGIN      
        1 mvsdsapraq glqyrcrtrh rafvfsdses reemdeyilg prhrgvvvhp pkrptclaqd
       61 kvrnvkglwm larqattdel ldsiaecgge eeieklrken eelrqlnmkl qnqllrflsa
      121 dalpepapsv amaaasahlh lwhqhlqqip lwprhsrllp ihpwpplghh qapfl
//
LOCUS       XP_077505185             175 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114977 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505185
VERSION     XP_077505185.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649059.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..175
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..175
                     /product="uncharacterized protein LOC144114977 isoform X1"
                     /calculated_mol_wt=19998
     Region          78..>114
                     /region_name="DivIVA"
                     /note="Cell division septum initiation protein DivIVA,
                     interacts with FtsZ and MinD [Cell cycle control, cell
                     division, chromosome partitioning]; COG3599"
                     /db_xref="CDD:442818"
     CDS             1..175
                     /gene="LOC144114977"
                     /coded_by="XM_077649059.1:1149..1676"
                     /db_xref="GeneID:144114977"
ORIGIN      
        1 mvsdsapraq glqyrcrtrh rafvfsdses reemdeyilg prhrgvvvhp pkrptclaqd
       61 kvrnvkglwm larqattdel ldsiaecgge eeieklrken eelrqlnmkl qnqllrflsa
      121 dalpepapsv amaaasahlh lwhqhlqqip lwprhsrllp ihpwpplghh qapfl
//
LOCUS       XP_077505186             149 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114977 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505186
VERSION     XP_077505186.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649060.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..149
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..149
                     /product="uncharacterized protein LOC144114977 isoform X2"
                     /calculated_mol_wt=17006
     Region          52..>91
                     /region_name="DivIVA"
                     /note="Cell division septum initiation protein DivIVA,
                     interacts with FtsZ and MinD [Cell cycle control, cell
                     division, chromosome partitioning]; COG3599"
                     /db_xref="CDD:442818"
     CDS             1..149
                     /gene="LOC144114977"
                     /coded_by="XM_077649060.1:443..892"
                     /db_xref="GeneID:144114977"
ORIGIN      
        1 mtesreemde yilgprhrgv vvhppkrptc laqdkvrnvk glwmlarqat tdelldsiae
       61 cggeeeiekl rkeneelrql nmklqnqllr flsadalpep apsvamaaas ahlhlwhqhl
      121 qqiplwprhs rllpihpwpp lghhqapfl
//
LOCUS       XP_077505187            2444 aa            linear   INV 25-NOV-2025
DEFINITION  ATP-binding cassette sub-family A member 2-like [Amblyomma
            americanum].
ACCESSION   XP_077505187
VERSION     XP_077505187.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649061.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2444
                     /product="ATP-binding cassette sub-family A member 2-like"
                     /calculated_mol_wt=275919
     Region          1..2395
                     /region_name="rim_protein"
                     /note="retinal-specific rim ABC transporter; TIGR01257"
                     /db_xref="CDD:130324"
     CDS             1..2444
                     /gene="LOC144114980"
                     /coded_by="XM_077649061.1:362..7696"
                     /db_xref="GeneID:144114980"
ORIGIN      
        1 mgfclqlrll lwknytlkkr kplvllfelv iplvlffili girkkqpayp vgsssfpafp
       61 lpsagviavm qafcdngvrd sngfatfpns tvtpflerlk nvskhhnffs pgftlgdldl
      121 ipsifktive dpvalhdcfa qapamrvdyf lkdpdelkdf lstnlslpsr dldvifnssl
      181 dmkqvyffmf gqpfedfgdd plvplspvpk eqqhhfnarq alshppllls sptflpglql
      241 isfrhlefil kllrtyphes ahllavlrwv ldtdrshppp kinpeeaass lvalllspev
      301 ltasfceyhk lpnvfilnas atsesvtdlr lhfcnltske vallsselkr qvdlkkladt
      361 laldrwnlsv ahkrisklad dlhrfnlfet avqeiselal qlpqdachsl ndtnstllhn
      421 atvlddestp snsheildkv krqkpqygll riwlgmqrmv cgkveksees msseaedinl
      481 nigiseyqke qigilvhvly snpkvlytpn ntaanlivhk anetfklldt vtqyarkmln
      541 isrdirdyla qnssdyslsm lkhmrdnlrr yplvldvahs qalrefards gasviedkea
      601 flhrldvidn aacswislms glslnmfqgf steddlmayf knkayfdnvt ilasvlfkmd
      661 hngsmprhms ytirqnasft pttnlmrsrf wfpgprnwgy eyyqfgfvwl qdileramvn
      721 vyagrdvtep gtyihqfpyp cyiqdqflfm iehvmplcma iswvysvaml vqnvvyekek
      781 rlkevmktmg lnnavhwlaw fitsfiqmti taavltallk ygrvltysnp lifflvleif
      841 vianiafsfl vsvlyskakl aaacagiiyf ltyvpymyia vreeaahnni pawlkslasl
      901 lsttafglga kyfafyeevg vgvqwanigv spleddtfsl ahvalmmlid afiysllvwy
      961 ienvhpgsyg lprpwyfpvt hsywfgggrh dleygplrsf wqkllrkhvt hlsvteeeqa
     1021 camegraadn gffepdpsel plgvcidnlv kiykdgkkla vnklslnlye gqitsflghn
     1081 gagktttmsi ltglfpptsg yatiyghdir temdlirqsm gmcpqhnvlf deltveehlw
     1141 fyarlkhtpd snikdeteki iqdlglplkr fskvdclsgg mkrklsvaia fvggsrvvil
     1201 deptagvdpy srraiwdlil kykkertill sthhmdeadv lgdriavish gqlrccgtsl
     1261 flknnlgkgy hltlvkqppr hlsgdpvaec cektvtlfir qhvetavlvs etqhelhyil
     1321 plfelrkgsf eklfsalets lsdlgvssyg iknttleevf lkvaeeasrs dsenfdespi
     1381 kpkvvssskh dennscaeek qpltdfgysl aelsgssggd adeasqwngf tstgeepess
     1441 gswvgsgrrv egklllvqql saillkryyc tqrnwkglfs qillpaffvs iamsvaltap
     1501 hvedpppltl spsryynytq prgnvvpyaf reeprpkwnl dadaqhlaat lrlpsgvgat
     1561 cvllwplngs nsilvpssna nysersldwd mldafepecr svfvpglpld nfvpplptve
     1621 sptgdaadvt tsprtvfhpv niskplkegk erfypycqcs adktgyvcep ygfqdppmfk
     1681 altgdilldi ssqneheyyl yttdmyrlrr ygafsfgltr dyvpstfged apalfrkiav
     1741 rdvakvwynn kgyhamptyv nsinnailra nlppekghps tygitvvnhp mtdtsfllsk
     1801 dqilqgtdvl iaifiivams fvpasfvlfl vyerytkakh lqivsgvnpv vywlanyfwd
     1861 icsyvvpatc cvlillifdi paytsaknfp avlslflmyg wsitpvmypv sfvfkepsta
     1921 yiflivinlf vgitcivtsf llevfsyday lgevhhllka iflmfpnycl grglmdiafn
     1981 eyqnfflfkt grydrmrspf awdlvtrnll amalsgmlfl vltlllefnf flkpkkvtvp
     2041 etlnvaeded vqnerkrvls gqtdsaalcl vnltkiyqtr klgkhlavdr lclripkgec
     2101 fgllgvngag ksttfkmltg dteitsgdaf lngysvsrel nkaqqyigyc pqfdalydel
     2161 takehlrlys rfrgipvkee nkviewtlqk lgltpyadrv vgtysggnkr klstaiallg
     2221 gppviyldep ttgmdpytrr flwdliqdlv qggrsvilts hsmeecealc trlaimvngh
     2281 fkclgsiqhl knrfgegyci tvrtrgtshd aiaawfhrvf hnaalkeqhf nmlqyelkse
     2341 nislayvfcq meqalielpi eeysvcqntl dnvfinfvkq qsergrepkf wssagkrats
     2401 spakkgqlel plddtigdsd dedigerlqr sqshlaflnm dvdc
//
LOCUS       XP_077505188            2216 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114981 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505188
VERSION     XP_077505188.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649062.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2216
                     /product="uncharacterized protein LOC144114981 isoform X1"
                     /calculated_mol_wt=248462
     Region          <93..>365
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <1411..>1700
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          <1585..1883
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..2216
                     /gene="LOC144114981"
                     /coded_by="XM_077649062.1:153..6803"
                     /db_xref="GeneID:144114981"
ORIGIN      
        1 mdnpqsness aaesrpetnl dlpassmeap tsgshgavan qehslrnyeq niislrqenl
       61 rlrlrsfylq lqtktpaeen enefdvaify hqknvaeqiq kfvtelderd klldesrtkr
      121 eeleklvanl ihekaalede lckwkmktnv seaaqddnvk mvstlshvkk eledkvkqle
      181 qqldelkdkk vpslkrqldt rtaalqaisa haakqeealk slhnvhslcs liqpkteafl
      241 qliddslaag diqatqrakn dfqrelskis sehcsfqlds teqpcsvaaf sdrvwkleae
      301 lqsrenliec lnsdiaclts rleatqtrcl eyetemkras eihqkicaek dklldsvqrk
      361 mqemerhaqe glspksrtse lqlecnrlhi avleksrfin ellsernkat veteksfhel
      421 llsmkqkdql iemmqtkfeh aeegeavien eglrqpnpad flletcegqp kpaepgtplg
      481 terpsfqgss paspegpdep tvfylsqsvp revkvlyqil sdlaqesgim pelstvaltn
      541 isciqkvtei lksahekglr fslpiqstiv nmdsldhcep derglaawep tvlvklgekl
      601 qqlaylfavd gpvvskwqma dvvqlldtvl ekakqeaank lflhrspedl ppvlfgkggq
      661 pkeheelths amlsphlrne rghsdisdyq eellqdlgsa lkcvgdlhne vkalpltpts
      721 netrkstgst delifavdss gqlvpvpvdq aghfekdass qteepcdnle slkselnnak
      781 aqvkflqksl kdsfsvkvte sptgpnaqpl ieidqnlpvl gslgsqsprk sglsanrrwr
      841 kqltnqkmse fglsenvfel qeeifflvlr iedlerqlkd nfevvskhkd eslseqagva
      901 vvqaleekna alqeqlhqmq evstvllcrl eelaafleql laydetgslg svtlsadmva
      961 kirqlvadsk dfslsfsqsf lgsdnnsals dhllkrgsfd rsmqsilsrs kgkndsisvi
     1021 dkpqsckldi ntippcpedi afmedelktl rdrvqeqqse issltrrlee egseraspaa
     1081 ggrcpsrrhp gtpaslcege dtdaswryqd qvvlvqasds ddillllner glpenvylrs
     1141 twqkqalnaa eslpavcsav aaeeadadad yssssspsll rwkearnsal engapqeplv
     1201 pigrfqcwss dsdiwsepdr svseqrmggv crkewnkqpr rspketrkam aggrqeqpgs
     1261 dtgakqakds wpevakgkrl scptdggrsk ravsashtka qklekllrri qhcnsrledt
     1321 ltvhkelcqk llripsgdgp kdasesqqpk dlteslvpsm rklqfrleem vinnevlqei
     1381 ltehvasgka vltepctpgg aganskegaa eelnqckkel ackerredil kramvheqda
     1441 kvqlekelad cqkelqslhe ahcalekqyk tavqrnsdla lrdpqrrssf eksrlerarg
     1501 ssferpdgee mklrdenhtl taelrqqaqe iarlgkeeal hkdevsnlsq ektvlsqklq
     1561 avqhehevll esssnkisel vtklgalstq nknqheeirn lremgesisr eniklkhdld
     1621 atsseleglr venqdlretl lalrkqesll rknlekavde qqaakkisde ysqsnkllrm
     1681 ekdqlgakis dlssrldale cekqslyeek qrlilevsag edevervnsk lqsceeecnn
     1741 lrtqksevea elqacnnklq efehlaeqlr eelsatkgkl esmdtrcnhl eqelgaskea
     1801 hkqllwkrde lqrtleavgr eaqqskslar msldkkhvma fmelqdksrs lqnvvmelkl
     1861 kleramyenk clrlelrvne qfqsgnssga vvgtpdslrs fataasnasp prlsphqvpv
     1921 dssqrtppvv gqhscshsrs yhtspdlgid sdptpehdvy staveptyve hwqhrmgpdw
     1981 sissvhsapd afggscllcq qgkdspaswk fkanatygge qldhpstqsp sksgfpvisc
     2041 slsspgvhlc aivqlsdhel lkkrvdesic lirrveasvq qaldaladfa sdcteppecn
     2101 rllqdaergc qsikmclmds fklicsftma qmpeakeehs fehkqlrmel nklqekhsqq
     2161 saelqnaldq isslkekkeg meraisrqln ktkmvlkqtk gnlnvlrard akpekk
//
LOCUS       XP_077505189            2215 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114981 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505189
VERSION     XP_077505189.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649063.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2215
                     /product="uncharacterized protein LOC144114981 isoform X2"
                     /calculated_mol_wt=248334
     Region          <92..>364
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <1410..>1699
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          <1584..1882
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..2215
                     /gene="LOC144114981"
                     /coded_by="XM_077649063.1:153..6800"
                     /db_xref="GeneID:144114981"
ORIGIN      
        1 mdnpqsness aaesrpetnl dlpassmeap tsgshgavan qehslrnyeq niislrqenl
       61 rlrlrsfylq lqtktpaeen enefdvaify hknvaeqiqk fvtelderdk lldesrtkre
      121 eleklvanli hekaaledel ckwkmktnvs eaaqddnvkm vstlshvkke ledkvkqleq
      181 qldelkdkkv pslkrqldtr taalqaisah aakqeealks lhnvhslcsl iqpkteaflq
      241 liddslaagd iqatqraknd fqrelskiss ehcsfqldst eqpcsvaafs drvwkleael
      301 qsrenliecl nsdiacltsr leatqtrcle yetemkrase ihqkicaekd klldsvqrkm
      361 qemerhaqeg lspksrtsel qlecnrlhia vleksrfine llsernkatv eteksfhell
      421 lsmkqkdqli emmqtkfeha eegeaviene glrqpnpadf lletcegqpk paepgtplgt
      481 erpsfqgssp aspegpdept vfylsqsvpr evkvlyqils dlaqesgimp elstvaltni
      541 sciqkvteil ksahekglrf slpiqstivn mdsldhcepd erglaawept vlvklgeklq
      601 qlaylfavdg pvvskwqmad vvqlldtvle kakqeaankl flhrspedlp pvlfgkggqp
      661 keheelthsa mlsphlrner ghsdisdyqe ellqdlgsal kcvgdlhnev kalpltptsn
      721 etrkstgstd elifavdssg qlvpvpvdqa ghfekdassq teepcdnles lkselnnaka
      781 qvkflqkslk dsfsvkvtes ptgpnaqpli eidqnlpvlg slgsqsprks glsanrrwrk
      841 qltnqkmsef glsenvfelq eeifflvlri edlerqlkdn fevvskhkde slseqagvav
      901 vqaleeknaa lqeqlhqmqe vstvllcrle elaafleqll aydetgslgs vtlsadmvak
      961 irqlvadskd fslsfsqsfl gsdnnsalsd hllkrgsfdr smqsilsrsk gkndsisvid
     1021 kpqsckldin tippcpedia fmedelktlr drvqeqqsei ssltrrleee gseraspaag
     1081 grcpsrrhpg tpaslceged tdaswryqdq vvlvqasdsd dillllnerg lpenvylrst
     1141 wqkqalnaae slpavcsava aeeadadady ssssspsllr wkearnsale ngapqeplvp
     1201 igrfqcwssd sdiwsepdrs vseqrmggvc rkewnkqprr spketrkama ggrqeqpgsd
     1261 tgakqakdsw pevakgkrls cptdggrskr avsashtkaq klekllrriq hcnsrledtl
     1321 tvhkelcqkl lripsgdgpk dasesqqpkd lteslvpsmr klqfrleemv innevlqeil
     1381 tehvasgkav ltepctpgga ganskegaae elnqckkela ckerredilk ramvheqdak
     1441 vqlekeladc qkelqslhea hcalekqykt avqrnsdlal rdpqrrssfe ksrlerargs
     1501 sferpdgeem klrdenhtlt aelrqqaqei arlgkeealh kdevsnlsqe ktvlsqklqa
     1561 vqhehevlle sssnkiselv tklgalstqn knqheeirnl remgesisre niklkhdlda
     1621 tsseleglrv enqdlretll alrkqesllr knlekavdeq qaakkisdey sqsnkllrme
     1681 kdqlgakisd lssrldalec ekqslyeekq rlilevsage devervnskl qsceeecnnl
     1741 rtqkseveae lqacnnklqe fehlaeqlre elsatkgkle smdtrcnhle qelgaskeah
     1801 kqllwkrdel qrtleavgre aqqskslarm sldkkhvmaf melqdksrsl qnvvmelklk
     1861 leramyenkc lrlelrvneq fqsgnssgav vgtpdslrsf ataasnaspp rlsphqvpvd
     1921 ssqrtppvvg qhscshsrsy htspdlgids dptpehdvys taveptyveh wqhrmgpdws
     1981 issvhsapda fggscllcqq gkdspaswkf kanatyggeq ldhpstqsps ksgfpviscs
     2041 lsspgvhlca ivqlsdhell kkrvdesicl irrveasvqq aldaladfas dcteppecnr
     2101 llqdaergcq sikmclmdsf klicsftmaq mpeakeehsf ehkqlrmeln klqekhsqqs
     2161 aelqnaldqi sslkekkegm eraisrqlnk tkmvlkqtkg nlnvlrarda kpekk
//
LOCUS       XP_077505190            2209 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114981 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505190
VERSION     XP_077505190.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649064.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2209
                     /product="uncharacterized protein LOC144114981 isoform X3"
                     /calculated_mol_wt=247617
     Region          <86..>358
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <1404..>1693
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          <1578..1876
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..2209
                     /gene="LOC144114981"
                     /coded_by="XM_077649064.1:153..6782"
                     /db_xref="GeneID:144114981"
ORIGIN      
        1 mdnpqsness aaesrpetnl dlpassmeap tsgshgavan qehslrnyeq niislrqenl
       61 rlrlrsfylq lqtktpaeen enefqknvae qiqkfvteld erdklldesr tkreeleklv
      121 anlihekaal edelckwkmk tnvseaaqdd nvkmvstlsh vkkeledkvk qleqqldelk
      181 dkkvpslkrq ldtrtaalqa isahaakqee alkslhnvhs lcsliqpkte aflqliddsl
      241 aagdiqatqr akndfqrels kissehcsfq ldsteqpcsv aafsdrvwkl eaelqsrenl
      301 ieclnsdiac ltsrleatqt rcleyetemk raseihqkic aekdklldsv qrkmqemerh
      361 aqeglspksr tselqlecnr lhiavleksr finellsern katveteksf helllsmkqk
      421 dqliemmqtk fehaeegeav ieneglrqpn padflletce gqpkpaepgt plgterpsfq
      481 gsspaspegp deptvfylsq svprevkvly qilsdlaqes gimpelstva ltnisciqkv
      541 teilksahek glrfslpiqs tivnmdsldh cepderglaa weptvlvklg eklqqlaylf
      601 avdgpvvskw qmadvvqlld tvlekakqea anklflhrsp edlppvlfgk ggqpkeheel
      661 thsamlsphl rnerghsdis dyqeellqdl gsalkcvgdl hnevkalplt ptsnetrkst
      721 gstdelifav dssgqlvpvp vdqaghfekd assqteepcd nleslkseln nakaqvkflq
      781 kslkdsfsvk vtesptgpna qplieidqnl pvlgslgsqs prksglsanr rwrkqltnqk
      841 msefglsenv felqeeiffl vlriedlerq lkdnfevvsk hkdeslseqa gvavvqalee
      901 knaalqeqlh qmqevstvll crleelaafl eqllaydetg slgsvtlsad mvakirqlva
      961 dskdfslsfs qsflgsdnns alsdhllkrg sfdrsmqsil srskgkndsi svidkpqsck
     1021 ldintippcp ediafmedel ktlrdrvqeq qseissltrr leeegseras paaggrcpsr
     1081 rhpgtpaslc egedtdaswr yqdqvvlvqa sdsddillll nerglpenvy lrstwqkqal
     1141 naaeslpavc savaaeeada dadysssssp sllrwkearn salengapqe plvpigrfqc
     1201 wssdsdiwse pdrsvseqrm ggvcrkewnk qprrspketr kamaggrqeq pgsdtgakqa
     1261 kdswpevakg krlscptdgg rskravsash tkaqklekll rriqhcnsrl edtltvhkel
     1321 cqkllripsg dgpkdasesq qpkdlteslv psmrklqfrl eemvinnevl qeiltehvas
     1381 gkavltepct pggaganske gaaeelnqck kelackerre dilkramvhe qdakvqleke
     1441 ladcqkelqs lheahcalek qyktavqrns dlalrdpqrr ssfeksrler argssferpd
     1501 geemklrden htltaelrqq aqeiarlgke ealhkdevsn lsqektvlsq klqavqhehe
     1561 vllesssnki selvtklgal stqnknqhee irnlremges isreniklkh dldatssele
     1621 glrvenqdlr etllalrkqe sllrknleka vdeqqaakki sdeysqsnkl lrmekdqlga
     1681 kisdlssrld alecekqsly eekqrlilev sagedeverv nsklqsceee cnnlrtqkse
     1741 veaelqacnn klqefehlae qlreelsatk gklesmdtrc nhleqelgas keahkqllwk
     1801 rdelqrtlea vgreaqqsks larmsldkkh vmafmelqdk srslqnvvme lklkleramy
     1861 enkclrlelr vneqfqsgns sgavvgtpds lrsfataasn aspprlsphq vpvdssqrtp
     1921 pvvgqhscsh srsyhtspdl gidsdptpeh dvystavept yvehwqhrmg pdwsissvhs
     1981 apdafggscl lcqqgkdspa swkfkanaty ggeqldhpst qspsksgfpv iscslsspgv
     2041 hlcaivqlsd hellkkrvde siclirrvea svqqaldala dfasdctepp ecnrllqdae
     2101 rgcqsikmcl mdsfklicsf tmaqmpeake ehsfehkqlr melnklqekh sqqsaelqna
     2161 ldqisslkek kegmeraisr qlnktkmvlk qtkgnlnvlr ardakpekk
//
LOCUS       XP_077505191            2208 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114981 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505191
VERSION     XP_077505191.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649065.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2208
                     /product="uncharacterized protein LOC144114981 isoform X4"
                     /calculated_mol_wt=247488
     Region          <71..>357
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <1403..>1692
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          <1577..1875
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..2208
                     /gene="LOC144114981"
                     /coded_by="XM_077649065.1:153..6779"
                     /db_xref="GeneID:144114981"
ORIGIN      
        1 mdnpqsness aaesrpetnl dlpassmeap tsgshgavan qehslrnyeq niislrqenl
       61 rlrlrsfylq lqtktpaeen enefknvaeq iqkfvtelde rdklldesrt kreeleklva
      121 nlihekaale delckwkmkt nvseaaqddn vkmvstlshv kkeledkvkq leqqldelkd
      181 kkvpslkrql dtrtaalqai sahaakqeea lkslhnvhsl csliqpktea flqliddsla
      241 agdiqatqra kndfqrelsk issehcsfql dsteqpcsva afsdrvwkle aelqsrenli
      301 eclnsdiacl tsrleatqtr cleyetemkr aseihqkica ekdklldsvq rkmqemerha
      361 qeglspksrt selqlecnrl hiavleksrf inellsernk atveteksfh elllsmkqkd
      421 qliemmqtkf ehaeegeavi eneglrqpnp adflletceg qpkpaepgtp lgterpsfqg
      481 sspaspegpd eptvfylsqs vprevkvlyq ilsdlaqesg impelstval tnisciqkvt
      541 eilksahekg lrfslpiqst ivnmdsldhc epderglaaw eptvlvklge klqqlaylfa
      601 vdgpvvskwq madvvqlldt vlekakqeaa nklflhrspe dlppvlfgkg gqpkeheelt
      661 hsamlsphlr nerghsdisd yqeellqdlg salkcvgdlh nevkalpltp tsnetrkstg
      721 stdelifavd ssgqlvpvpv dqaghfekda ssqteepcdn leslkselnn akaqvkflqk
      781 slkdsfsvkv tesptgpnaq plieidqnlp vlgslgsqsp rksglsanrr wrkqltnqkm
      841 sefglsenvf elqeeifflv lriedlerql kdnfevvskh kdeslseqag vavvqaleek
      901 naalqeqlhq mqevstvllc rleelaafle qllaydetgs lgsvtlsadm vakirqlvad
      961 skdfslsfsq sflgsdnnsa lsdhllkrgs fdrsmqsils rskgkndsis vidkpqsckl
     1021 dintippcpe diafmedelk tlrdrvqeqq seissltrrl eeegserasp aaggrcpsrr
     1081 hpgtpaslce gedtdaswry qdqvvlvqas dsddilllln erglpenvyl rstwqkqaln
     1141 aaeslpavcs avaaeeadad adysssssps llrwkearns alengapqep lvpigrfqcw
     1201 ssdsdiwsep drsvseqrmg gvcrkewnkq prrspketrk amaggrqeqp gsdtgakqak
     1261 dswpevakgk rlscptdggr skravsasht kaqklekllr riqhcnsrle dtltvhkelc
     1321 qkllripsgd gpkdasesqq pkdlteslvp smrklqfrle emvinnevlq eiltehvasg
     1381 kavltepctp ggaganskeg aaeelnqckk elackerred ilkramvheq dakvqlekel
     1441 adcqkelqsl heahcalekq yktavqrnsd lalrdpqrrs sfeksrlera rgssferpdg
     1501 eemklrdenh tltaelrqqa qeiarlgkee alhkdevsnl sqektvlsqk lqavqhehev
     1561 llesssnkis elvtklgals tqnknqheei rnlremgesi sreniklkhd ldatsseleg
     1621 lrvenqdlre tllalrkqes llrknlekav deqqaakkis deysqsnkll rmekdqlgak
     1681 isdlssrlda lecekqslye ekqrlilevs agedevervn sklqsceeec nnlrtqksev
     1741 eaelqacnnk lqefehlaeq lreelsatkg klesmdtrcn hleqelgask eahkqllwkr
     1801 delqrtleav greaqqsksl armsldkkhv mafmelqdks rslqnvvmel klkleramye
     1861 nkclrlelrv neqfqsgnss gavvgtpdsl rsfataasna spprlsphqv pvdssqrtpp
     1921 vvgqhscshs rsyhtspdlg idsdptpehd vystavepty vehwqhrmgp dwsissvhsa
     1981 pdafggscll cqqgkdspas wkfkanatyg geqldhpstq spsksgfpvi scslsspgvh
     2041 lcaivqlsdh ellkkrvdes iclirrveas vqqaldalad fasdcteppe cnrllqdaer
     2101 gcqsikmclm dsfklicsft maqmpeakee hsfehkqlrm elnklqekhs qqsaelqnal
     2161 dqisslkekk egmeraisrq lnktkmvlkq tkgnlnvlra rdakpekk
//
LOCUS       XP_077505192            2196 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114981 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077505192
VERSION     XP_077505192.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649066.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2196
                     /product="uncharacterized protein LOC144114981 isoform X5"
                     /calculated_mol_wt=245948
     Region          <73..>345
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <1391..>1680
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          <1565..1863
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..2196
                     /gene="LOC144114981"
                     /coded_by="XM_077649066.1:153..6743"
                     /db_xref="GeneID:144114981"
ORIGIN      
        1 mdnpqsness aaesrpetnl dlpassmeap tsgshgavan qehslrnyeq lqtktpaeen
       61 enefdvaify hqknvaeqiq kfvtelderd klldesrtkr eeleklvanl ihekaalede
      121 lckwkmktnv seaaqddnvk mvstlshvkk eledkvkqle qqldelkdkk vpslkrqldt
      181 rtaalqaisa haakqeealk slhnvhslcs liqpkteafl qliddslaag diqatqrakn
      241 dfqrelskis sehcsfqlds teqpcsvaaf sdrvwkleae lqsrenliec lnsdiaclts
      301 rleatqtrcl eyetemkras eihqkicaek dklldsvqrk mqemerhaqe glspksrtse
      361 lqlecnrlhi avleksrfin ellsernkat veteksfhel llsmkqkdql iemmqtkfeh
      421 aeegeavien eglrqpnpad flletcegqp kpaepgtplg terpsfqgss paspegpdep
      481 tvfylsqsvp revkvlyqil sdlaqesgim pelstvaltn isciqkvtei lksahekglr
      541 fslpiqstiv nmdsldhcep derglaawep tvlvklgekl qqlaylfavd gpvvskwqma
      601 dvvqlldtvl ekakqeaank lflhrspedl ppvlfgkggq pkeheelths amlsphlrne
      661 rghsdisdyq eellqdlgsa lkcvgdlhne vkalpltpts netrkstgst delifavdss
      721 gqlvpvpvdq aghfekdass qteepcdnle slkselnnak aqvkflqksl kdsfsvkvte
      781 sptgpnaqpl ieidqnlpvl gslgsqsprk sglsanrrwr kqltnqkmse fglsenvfel
      841 qeeifflvlr iedlerqlkd nfevvskhkd eslseqagva vvqaleekna alqeqlhqmq
      901 evstvllcrl eelaafleql laydetgslg svtlsadmva kirqlvadsk dfslsfsqsf
      961 lgsdnnsals dhllkrgsfd rsmqsilsrs kgkndsisvi dkpqsckldi ntippcpedi
     1021 afmedelktl rdrvqeqqse issltrrlee egseraspaa ggrcpsrrhp gtpaslcege
     1081 dtdaswryqd qvvlvqasds ddillllner glpenvylrs twqkqalnaa eslpavcsav
     1141 aaeeadadad yssssspsll rwkearnsal engapqeplv pigrfqcwss dsdiwsepdr
     1201 svseqrmggv crkewnkqpr rspketrkam aggrqeqpgs dtgakqakds wpevakgkrl
     1261 scptdggrsk ravsashtka qklekllrri qhcnsrledt ltvhkelcqk llripsgdgp
     1321 kdasesqqpk dlteslvpsm rklqfrleem vinnevlqei ltehvasgka vltepctpgg
     1381 aganskegaa eelnqckkel ackerredil kramvheqda kvqlekelad cqkelqslhe
     1441 ahcalekqyk tavqrnsdla lrdpqrrssf eksrlerarg ssferpdgee mklrdenhtl
     1501 taelrqqaqe iarlgkeeal hkdevsnlsq ektvlsqklq avqhehevll esssnkisel
     1561 vtklgalstq nknqheeirn lremgesisr eniklkhdld atsseleglr venqdlretl
     1621 lalrkqesll rknlekavde qqaakkisde ysqsnkllrm ekdqlgakis dlssrldale
     1681 cekqslyeek qrlilevsag edevervnsk lqsceeecnn lrtqksevea elqacnnklq
     1741 efehlaeqlr eelsatkgkl esmdtrcnhl eqelgaskea hkqllwkrde lqrtleavgr
     1801 eaqqskslar msldkkhvma fmelqdksrs lqnvvmelkl kleramyenk clrlelrvne
     1861 qfqsgnssga vvgtpdslrs fataasnasp prlsphqvpv dssqrtppvv gqhscshsrs
     1921 yhtspdlgid sdptpehdvy staveptyve hwqhrmgpdw sissvhsapd afggscllcq
     1981 qgkdspaswk fkanatygge qldhpstqsp sksgfpvisc slsspgvhlc aivqlsdhel
     2041 lkkrvdesic lirrveasvq qaldaladfa sdcteppecn rllqdaergc qsikmclmds
     2101 fklicsftma qmpeakeehs fehkqlrmel nklqekhsqq saelqnaldq isslkekkeg
     2161 meraisrqln ktkmvlkqtk gnlnvlrard akpekk
//
LOCUS       XP_077505193            2195 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114981 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077505193
VERSION     XP_077505193.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649067.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2195
                     /product="uncharacterized protein LOC144114981 isoform X6"
                     /calculated_mol_wt=245819
     Region          <72..>344
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <1390..>1679
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          <1564..1862
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..2195
                     /gene="LOC144114981"
                     /coded_by="XM_077649067.1:153..6740"
                     /db_xref="GeneID:144114981"
ORIGIN      
        1 mdnpqsness aaesrpetnl dlpassmeap tsgshgavan qehslrnyeq lqtktpaeen
       61 enefdvaify hknvaeqiqk fvtelderdk lldesrtkre eleklvanli hekaaledel
      121 ckwkmktnvs eaaqddnvkm vstlshvkke ledkvkqleq qldelkdkkv pslkrqldtr
      181 taalqaisah aakqeealks lhnvhslcsl iqpkteaflq liddslaagd iqatqraknd
      241 fqrelskiss ehcsfqldst eqpcsvaafs drvwkleael qsrenliecl nsdiacltsr
      301 leatqtrcle yetemkrase ihqkicaekd klldsvqrkm qemerhaqeg lspksrtsel
      361 qlecnrlhia vleksrfine llsernkatv eteksfhell lsmkqkdqli emmqtkfeha
      421 eegeaviene glrqpnpadf lletcegqpk paepgtplgt erpsfqgssp aspegpdept
      481 vfylsqsvpr evkvlyqils dlaqesgimp elstvaltni sciqkvteil ksahekglrf
      541 slpiqstivn mdsldhcepd erglaawept vlvklgeklq qlaylfavdg pvvskwqmad
      601 vvqlldtvle kakqeaankl flhrspedlp pvlfgkggqp keheelthsa mlsphlrner
      661 ghsdisdyqe ellqdlgsal kcvgdlhnev kalpltptsn etrkstgstd elifavdssg
      721 qlvpvpvdqa ghfekdassq teepcdnles lkselnnaka qvkflqkslk dsfsvkvtes
      781 ptgpnaqpli eidqnlpvlg slgsqsprks glsanrrwrk qltnqkmsef glsenvfelq
      841 eeifflvlri edlerqlkdn fevvskhkde slseqagvav vqaleeknaa lqeqlhqmqe
      901 vstvllcrle elaafleqll aydetgslgs vtlsadmvak irqlvadskd fslsfsqsfl
      961 gsdnnsalsd hllkrgsfdr smqsilsrsk gkndsisvid kpqsckldin tippcpedia
     1021 fmedelktlr drvqeqqsei ssltrrleee gseraspaag grcpsrrhpg tpaslceged
     1081 tdaswryqdq vvlvqasdsd dillllnerg lpenvylrst wqkqalnaae slpavcsava
     1141 aeeadadady ssssspsllr wkearnsale ngapqeplvp igrfqcwssd sdiwsepdrs
     1201 vseqrmggvc rkewnkqprr spketrkama ggrqeqpgsd tgakqakdsw pevakgkrls
     1261 cptdggrskr avsashtkaq klekllrriq hcnsrledtl tvhkelcqkl lripsgdgpk
     1321 dasesqqpkd lteslvpsmr klqfrleemv innevlqeil tehvasgkav ltepctpgga
     1381 ganskegaae elnqckkela ckerredilk ramvheqdak vqlekeladc qkelqslhea
     1441 hcalekqykt avqrnsdlal rdpqrrssfe ksrlerargs sferpdgeem klrdenhtlt
     1501 aelrqqaqei arlgkeealh kdevsnlsqe ktvlsqklqa vqhehevlle sssnkiselv
     1561 tklgalstqn knqheeirnl remgesisre niklkhdlda tsseleglrv enqdlretll
     1621 alrkqesllr knlekavdeq qaakkisdey sqsnkllrme kdqlgakisd lssrldalec
     1681 ekqslyeekq rlilevsage devervnskl qsceeecnnl rtqkseveae lqacnnklqe
     1741 fehlaeqlre elsatkgkle smdtrcnhle qelgaskeah kqllwkrdel qrtleavgre
     1801 aqqskslarm sldkkhvmaf melqdksrsl qnvvmelklk leramyenkc lrlelrvneq
     1861 fqsgnssgav vgtpdslrsf ataasnaspp rlsphqvpvd ssqrtppvvg qhscshsrsy
     1921 htspdlgids dptpehdvys taveptyveh wqhrmgpdws issvhsapda fggscllcqq
     1981 gkdspaswkf kanatyggeq ldhpstqsps ksgfpviscs lsspgvhlca ivqlsdhell
     2041 kkrvdesicl irrveasvqq aldaladfas dcteppecnr llqdaergcq sikmclmdsf
     2101 klicsftmaq mpeakeehsf ehkqlrmeln klqekhsqqs aelqnaldqi sslkekkegm
     2161 eraisrqlnk tkmvlkqtkg nlnvlrarda kpekk
//
LOCUS       XP_077505194            2190 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114981 isoform X7 [Amblyomma
            americanum].
ACCESSION   XP_077505194
VERSION     XP_077505194.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649068.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2190
                     /product="uncharacterized protein LOC144114981 isoform X7"
                     /calculated_mol_wt=245733
     Region          <67..>339
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <1385..>1674
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          <1559..1857
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..2190
                     /gene="LOC144114981"
                     /coded_by="XM_077649068.1:199..6771"
                     /db_xref="GeneID:144114981"
ORIGIN      
        1 meaptsgshg avanqehslr nyeqniislr qenlrlrlrs fylqlqtktp aeenenefdv
       61 aifyhqknva eqiqkfvtel derdklldes rtkreelekl vanlihekaa ledelckwkm
      121 ktnvseaaqd dnvkmvstls hvkkeledkv kqleqqldel kdkkvpslkr qldtrtaalq
      181 aisahaakqe ealkslhnvh slcsliqpkt eaflqlidds laagdiqatq rakndfqrel
      241 skissehcsf qldsteqpcs vaafsdrvwk leaelqsren lieclnsdia cltsrleatq
      301 trcleyetem kraseihqki caekdkllds vqrkmqemer haqeglspks rtselqlecn
      361 rlhiavleks rfinellser nkatveteks fhelllsmkq kdqliemmqt kfehaeegea
      421 vieneglrqp npadflletc egqpkpaepg tplgterpsf qgsspaspeg pdeptvfyls
      481 qsvprevkvl yqilsdlaqe sgimpelstv altnisciqk vteilksahe kglrfslpiq
      541 stivnmdsld hcepdergla aweptvlvkl geklqqlayl favdgpvvsk wqmadvvqll
      601 dtvlekakqe aanklflhrs pedlppvlfg kggqpkehee lthsamlsph lrnerghsdi
      661 sdyqeellqd lgsalkcvgd lhnevkalpl tptsnetrks tgstdelifa vdssgqlvpv
      721 pvdqaghfek dassqteepc dnleslksel nnakaqvkfl qkslkdsfsv kvtesptgpn
      781 aqplieidqn lpvlgslgsq sprksglsan rrwrkqltnq kmsefglsen vfelqeeiff
      841 lvlriedler qlkdnfevvs khkdeslseq agvavvqale eknaalqeql hqmqevstvl
      901 lcrleelaaf leqllaydet gslgsvtlsa dmvakirqlv adskdfslsf sqsflgsdnn
      961 salsdhllkr gsfdrsmqsi lsrskgknds isvidkpqsc kldintippc pediafmede
     1021 lktlrdrvqe qqseissltr rleeegsera spaaggrcps rrhpgtpasl cegedtdasw
     1081 ryqdqvvlvq asdsddilll lnerglpenv ylrstwqkqa lnaaeslpav csavaaeead
     1141 adadysssss psllrwkear nsalengapq eplvpigrfq cwssdsdiws epdrsvseqr
     1201 mggvcrkewn kqprrspket rkamaggrqe qpgsdtgakq akdswpevak gkrlscptdg
     1261 grskravsas htkaqklekl lrriqhcnsr ledtltvhke lcqkllrips gdgpkdases
     1321 qqpkdltesl vpsmrklqfr leemvinnev lqeiltehva sgkavltepc tpggagansk
     1381 egaaeelnqc kkelackerr edilkramvh eqdakvqlek eladcqkelq slheahcale
     1441 kqyktavqrn sdlalrdpqr rssfeksrle rargssferp dgeemklrde nhtltaelrq
     1501 qaqeiarlgk eealhkdevs nlsqektvls qklqavqheh evllesssnk iselvtklga
     1561 lstqnknqhe eirnlremge sisreniklk hdldatssel eglrvenqdl retllalrkq
     1621 esllrknlek avdeqqaakk isdeysqsnk llrmekdqlg akisdlssrl dalecekqsl
     1681 yeekqrlile vsagedever vnsklqscee ecnnlrtqks eveaelqacn nklqefehla
     1741 eqlreelsat kgklesmdtr cnhleqelga skeahkqllw krdelqrtle avgreaqqsk
     1801 slarmsldkk hvmafmelqd ksrslqnvvm elklkleram yenkclrlel rvneqfqsgn
     1861 ssgavvgtpd slrsfataas naspprlsph qvpvdssqrt ppvvgqhscs hsrsyhtspd
     1921 lgidsdptpe hdvystavep tyvehwqhrm gpdwsissvh sapdafggsc llcqqgkdsp
     1981 aswkfkanat yggeqldhps tqspsksgfp viscslsspg vhlcaivqls dhellkkrvd
     2041 esiclirrve asvqqaldal adfasdctep pecnrllqda ergcqsikmc lmdsfklics
     2101 ftmaqmpeak eehsfehkql rmelnklqek hsqqsaelqn aldqisslke kkegmerais
     2161 rqlnktkmvl kqtkgnlnvl rardakpekk
//
LOCUS       XP_077505195            2189 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114981 isoform X8 [Amblyomma
            americanum].
ACCESSION   XP_077505195
VERSION     XP_077505195.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649069.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2189
                     /product="uncharacterized protein LOC144114981 isoform X8"
                     /calculated_mol_wt=245102
     Region          <66..>338
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <1384..>1673
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          <1558..1856
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..2189
                     /gene="LOC144114981"
                     /coded_by="XM_077649069.1:153..6722"
                     /db_xref="GeneID:144114981"
ORIGIN      
        1 mdnpqsness aaesrpetnl dlpassmeap tsgshgavan qehslrnyeq lqtktpaeen
       61 enefqknvae qiqkfvteld erdklldesr tkreeleklv anlihekaal edelckwkmk
      121 tnvseaaqdd nvkmvstlsh vkkeledkvk qleqqldelk dkkvpslkrq ldtrtaalqa
      181 isahaakqee alkslhnvhs lcsliqpkte aflqliddsl aagdiqatqr akndfqrels
      241 kissehcsfq ldsteqpcsv aafsdrvwkl eaelqsrenl ieclnsdiac ltsrleatqt
      301 rcleyetemk raseihqkic aekdklldsv qrkmqemerh aqeglspksr tselqlecnr
      361 lhiavleksr finellsern katveteksf helllsmkqk dqliemmqtk fehaeegeav
      421 ieneglrqpn padflletce gqpkpaepgt plgterpsfq gsspaspegp deptvfylsq
      481 svprevkvly qilsdlaqes gimpelstva ltnisciqkv teilksahek glrfslpiqs
      541 tivnmdsldh cepderglaa weptvlvklg eklqqlaylf avdgpvvskw qmadvvqlld
      601 tvlekakqea anklflhrsp edlppvlfgk ggqpkeheel thsamlsphl rnerghsdis
      661 dyqeellqdl gsalkcvgdl hnevkalplt ptsnetrkst gstdelifav dssgqlvpvp
      721 vdqaghfekd assqteepcd nleslkseln nakaqvkflq kslkdsfsvk vtesptgpna
      781 qplieidqnl pvlgslgsqs prksglsanr rwrkqltnqk msefglsenv felqeeiffl
      841 vlriedlerq lkdnfevvsk hkdeslseqa gvavvqalee knaalqeqlh qmqevstvll
      901 crleelaafl eqllaydetg slgsvtlsad mvakirqlva dskdfslsfs qsflgsdnns
      961 alsdhllkrg sfdrsmqsil srskgkndsi svidkpqsck ldintippcp ediafmedel
     1021 ktlrdrvqeq qseissltrr leeegseras paaggrcpsr rhpgtpaslc egedtdaswr
     1081 yqdqvvlvqa sdsddillll nerglpenvy lrstwqkqal naaeslpavc savaaeeada
     1141 dadysssssp sllrwkearn salengapqe plvpigrfqc wssdsdiwse pdrsvseqrm
     1201 ggvcrkewnk qprrspketr kamaggrqeq pgsdtgakqa kdswpevakg krlscptdgg
     1261 rskravsash tkaqklekll rriqhcnsrl edtltvhkel cqkllripsg dgpkdasesq
     1321 qpkdlteslv psmrklqfrl eemvinnevl qeiltehvas gkavltepct pggaganske
     1381 gaaeelnqck kelackerre dilkramvhe qdakvqleke ladcqkelqs lheahcalek
     1441 qyktavqrns dlalrdpqrr ssfeksrler argssferpd geemklrden htltaelrqq
     1501 aqeiarlgke ealhkdevsn lsqektvlsq klqavqhehe vllesssnki selvtklgal
     1561 stqnknqhee irnlremges isreniklkh dldatssele glrvenqdlr etllalrkqe
     1621 sllrknleka vdeqqaakki sdeysqsnkl lrmekdqlga kisdlssrld alecekqsly
     1681 eekqrlilev sagedeverv nsklqsceee cnnlrtqkse veaelqacnn klqefehlae
     1741 qlreelsatk gklesmdtrc nhleqelgas keahkqllwk rdelqrtlea vgreaqqsks
     1801 larmsldkkh vmafmelqdk srslqnvvme lklkleramy enkclrlelr vneqfqsgns
     1861 sgavvgtpds lrsfataasn aspprlsphq vpvdssqrtp pvvgqhscsh srsyhtspdl
     1921 gidsdptpeh dvystavept yvehwqhrmg pdwsissvhs apdafggscl lcqqgkdspa
     1981 swkfkanaty ggeqldhpst qspsksgfpv iscslsspgv hlcaivqlsd hellkkrvde
     2041 siclirrvea svqqaldala dfasdctepp ecnrllqdae rgcqsikmcl mdsfklicsf
     2101 tmaqmpeake ehsfehkqlr melnklqekh sqqsaelqna ldqisslkek kegmeraisr
     2161 qlnktkmvlk qtkgnlnvlr ardakpekk
//
LOCUS       XP_077505196            2188 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114981 isoform X9 [Amblyomma
            americanum].
ACCESSION   XP_077505196
VERSION     XP_077505196.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649070.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2188
                     /product="uncharacterized protein LOC144114981 isoform X9"
                     /calculated_mol_wt=244973
     Region          <36..>337
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <1383..>1672
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          <1557..1855
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..2188
                     /gene="LOC144114981"
                     /coded_by="XM_077649070.1:153..6719"
                     /db_xref="GeneID:144114981"
ORIGIN      
        1 mdnpqsness aaesrpetnl dlpassmeap tsgshgavan qehslrnyeq lqtktpaeen
       61 enefknvaeq iqkfvtelde rdklldesrt kreeleklva nlihekaale delckwkmkt
      121 nvseaaqddn vkmvstlshv kkeledkvkq leqqldelkd kkvpslkrql dtrtaalqai
      181 sahaakqeea lkslhnvhsl csliqpktea flqliddsla agdiqatqra kndfqrelsk
      241 issehcsfql dsteqpcsva afsdrvwkle aelqsrenli eclnsdiacl tsrleatqtr
      301 cleyetemkr aseihqkica ekdklldsvq rkmqemerha qeglspksrt selqlecnrl
      361 hiavleksrf inellsernk atveteksfh elllsmkqkd qliemmqtkf ehaeegeavi
      421 eneglrqpnp adflletceg qpkpaepgtp lgterpsfqg sspaspegpd eptvfylsqs
      481 vprevkvlyq ilsdlaqesg impelstval tnisciqkvt eilksahekg lrfslpiqst
      541 ivnmdsldhc epderglaaw eptvlvklge klqqlaylfa vdgpvvskwq madvvqlldt
      601 vlekakqeaa nklflhrspe dlppvlfgkg gqpkeheelt hsamlsphlr nerghsdisd
      661 yqeellqdlg salkcvgdlh nevkalpltp tsnetrkstg stdelifavd ssgqlvpvpv
      721 dqaghfekda ssqteepcdn leslkselnn akaqvkflqk slkdsfsvkv tesptgpnaq
      781 plieidqnlp vlgslgsqsp rksglsanrr wrkqltnqkm sefglsenvf elqeeifflv
      841 lriedlerql kdnfevvskh kdeslseqag vavvqaleek naalqeqlhq mqevstvllc
      901 rleelaafle qllaydetgs lgsvtlsadm vakirqlvad skdfslsfsq sflgsdnnsa
      961 lsdhllkrgs fdrsmqsils rskgkndsis vidkpqsckl dintippcpe diafmedelk
     1021 tlrdrvqeqq seissltrrl eeegserasp aaggrcpsrr hpgtpaslce gedtdaswry
     1081 qdqvvlvqas dsddilllln erglpenvyl rstwqkqaln aaeslpavcs avaaeeadad
     1141 adysssssps llrwkearns alengapqep lvpigrfqcw ssdsdiwsep drsvseqrmg
     1201 gvcrkewnkq prrspketrk amaggrqeqp gsdtgakqak dswpevakgk rlscptdggr
     1261 skravsasht kaqklekllr riqhcnsrle dtltvhkelc qkllripsgd gpkdasesqq
     1321 pkdlteslvp smrklqfrle emvinnevlq eiltehvasg kavltepctp ggaganskeg
     1381 aaeelnqckk elackerred ilkramvheq dakvqlekel adcqkelqsl heahcalekq
     1441 yktavqrnsd lalrdpqrrs sfeksrlera rgssferpdg eemklrdenh tltaelrqqa
     1501 qeiarlgkee alhkdevsnl sqektvlsqk lqavqhehev llesssnkis elvtklgals
     1561 tqnknqheei rnlremgesi sreniklkhd ldatsseleg lrvenqdlre tllalrkqes
     1621 llrknlekav deqqaakkis deysqsnkll rmekdqlgak isdlssrlda lecekqslye
     1681 ekqrlilevs agedevervn sklqsceeec nnlrtqksev eaelqacnnk lqefehlaeq
     1741 lreelsatkg klesmdtrcn hleqelgask eahkqllwkr delqrtleav greaqqsksl
     1801 armsldkkhv mafmelqdks rslqnvvmel klkleramye nkclrlelrv neqfqsgnss
     1861 gavvgtpdsl rsfataasna spprlsphqv pvdssqrtpp vvgqhscshs rsyhtspdlg
     1921 idsdptpehd vystavepty vehwqhrmgp dwsissvhsa pdafggscll cqqgkdspas
     1981 wkfkanatyg geqldhpstq spsksgfpvi scslsspgvh lcaivqlsdh ellkkrvdes
     2041 iclirrveas vqqaldalad fasdcteppe cnrllqdaer gcqsikmclm dsfklicsft
     2101 maqmpeakee hsfehkqlrm elnklqekhs qqsaelqnal dqisslkekk egmeraisrq
     2161 lnktkmvlkq tkgnlnvlra rdakpekk
//
LOCUS       XP_077505197            2182 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114981 isoform X10 [Amblyomma
            americanum].
ACCESSION   XP_077505197
VERSION     XP_077505197.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649071.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2182
                     /product="uncharacterized protein LOC144114981 isoform
                     X10"
                     /calculated_mol_wt=244759
     Region          <45..>331
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <1377..>1666
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          <1551..1849
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..2182
                     /gene="LOC144114981"
                     /coded_by="XM_077649071.1:233..6781"
                     /db_xref="GeneID:144114981"
ORIGIN      
        1 meaptsgshg avanqehslr nyeqniislr qenlrlrlrs fylqlqtktp aeenenefkn
       61 vaeqiqkfvt elderdklld esrtkreele klvanlihek aaledelckw kmktnvseaa
      121 qddnvkmvst lshvkkeled kvkqleqqld elkdkkvpsl krqldtrtaa lqaisahaak
      181 qeealkslhn vhslcsliqp kteaflqlid dslaagdiqa tqrakndfqr elskissehc
      241 sfqldsteqp csvaafsdrv wkleaelqsr enlieclnsd iacltsrlea tqtrcleyet
      301 emkraseihq kicaekdkll dsvqrkmqem erhaqeglsp ksrtselqle cnrlhiavle
      361 ksrfinells ernkatvete ksfhelllsm kqkdqliemm qtkfehaeeg eavieneglr
      421 qpnpadflle tcegqpkpae pgtplgterp sfqgsspasp egpdeptvfy lsqsvprevk
      481 vlyqilsdla qesgimpels tvaltnisci qkvteilksa hekglrfslp iqstivnmds
      541 ldhcepderg laaweptvlv klgeklqqla ylfavdgpvv skwqmadvvq lldtvlekak
      601 qeaanklflh rspedlppvl fgkggqpkeh eelthsamls phlrnerghs disdyqeell
      661 qdlgsalkcv gdlhnevkal pltptsnetr kstgstdeli favdssgqlv pvpvdqaghf
      721 ekdassqtee pcdnleslks elnnakaqvk flqkslkdsf svkvtesptg pnaqplieid
      781 qnlpvlgslg sqsprksgls anrrwrkqlt nqkmsefgls envfelqeei fflvlriedl
      841 erqlkdnfev vskhkdesls eqagvavvqa leeknaalqe qlhqmqevst vllcrleela
      901 afleqllayd etgslgsvtl sadmvakirq lvadskdfsl sfsqsflgsd nnsalsdhll
      961 krgsfdrsmq silsrskgkn dsisvidkpq sckldintip pcpediafme delktlrdrv
     1021 qeqqseissl trrleeegse raspaaggrc psrrhpgtpa slcegedtda swryqdqvvl
     1081 vqasdsddil lllnerglpe nvylrstwqk qalnaaeslp avcsavaaee adadadysss
     1141 sspsllrwke arnsalenga pqeplvpigr fqcwssdsdi wsepdrsvse qrmggvcrke
     1201 wnkqprrspk etrkamaggr qeqpgsdtga kqakdswpev akgkrlscpt dggrskravs
     1261 ashtkaqkle kllrriqhcn srledtltvh kelcqkllri psgdgpkdas esqqpkdlte
     1321 slvpsmrklq frleemvinn evlqeilteh vasgkavlte pctpggagan skegaaeeln
     1381 qckkelacke rredilkram vheqdakvql ekeladcqke lqslheahca lekqyktavq
     1441 rnsdlalrdp qrrssfeksr lerargssfe rpdgeemklr denhtltael rqqaqeiarl
     1501 gkeealhkde vsnlsqektv lsqklqavqh ehevllesss nkiselvtkl galstqnknq
     1561 heeirnlrem gesisrenik lkhdldatss eleglrvenq dlretllalr kqesllrknl
     1621 ekavdeqqaa kkisdeysqs nkllrmekdq lgakisdlss rldalecekq slyeekqrli
     1681 levsagedev ervnsklqsc eeecnnlrtq kseveaelqa cnnklqefeh laeqlreels
     1741 atkgklesmd trcnhleqel gaskeahkql lwkrdelqrt leavgreaqq skslarmsld
     1801 kkhvmafmel qdksrslqnv vmelklkler amyenkclrl elrvneqfqs gnssgavvgt
     1861 pdslrsfata asnaspprls phqvpvdssq rtppvvgqhs cshsrsyhts pdlgidsdpt
     1921 pehdvystav eptyvehwqh rmgpdwsiss vhsapdafgg scllcqqgkd spaswkfkan
     1981 atyggeqldh pstqspsksg fpviscslss pgvhlcaivq lsdhellkkr vdesiclirr
     2041 veasvqqald aladfasdct eppecnrllq daergcqsik mclmdsfkli csftmaqmpe
     2101 akeehsfehk qlrmelnklq ekhsqqsael qnaldqissl kekkegmera isrqlnktkm
     2161 vlkqtkgnln vlrardakpe kk
//
LOCUS       XP_077505198            2175 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114981 isoform X11 [Amblyomma
            americanum].
ACCESSION   XP_077505198
VERSION     XP_077505198.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649072.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2175
                     /product="uncharacterized protein LOC144114981 isoform
                     X11"
                     /calculated_mol_wt=243796
     Region          <93..>329
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <1370..>1659
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          <1544..1842
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..2175
                     /gene="LOC144114981"
                     /coded_by="XM_077649072.1:153..6680"
                     /db_xref="GeneID:144114981"
ORIGIN      
        1 mdnpqsness aaesrpetnl dlpassmeap tsgshgavan qehslrnyeq niislrqenl
       61 rlrlrsfylq lqtktpaeen enefdvaify hqknvaeqiq kfvtelderd klldesrtkr
      121 eeleklvanl ihekaalede lckwkmktnv seaaqddnvk mvstlshvkk eledkvkqle
      181 qqldelkdkk vpslkrqldt rtaalqaisa haakqeealk slhnvhslcs liqpkteafl
      241 qliddslaag diqatqrakn dfqrelskcl nsdiacltsr leatqtrcle yetemkrase
      301 ihqkicaekd klldsvqrkm qemerhaqeg lspksrtsel qlecnrlhia vleksrfine
      361 llsernkatv eteksfhell lsmkqkdqli emmqtkfeha eegeaviene glrqpnpadf
      421 lletcegqpk paepgtplgt erpsfqgssp aspegpdept vfylsqsvpr evkvlyqils
      481 dlaqesgimp elstvaltni sciqkvteil ksahekglrf slpiqstivn mdsldhcepd
      541 erglaawept vlvklgeklq qlaylfavdg pvvskwqmad vvqlldtvle kakqeaankl
      601 flhrspedlp pvlfgkggqp keheelthsa mlsphlrner ghsdisdyqe ellqdlgsal
      661 kcvgdlhnev kalpltptsn etrkstgstd elifavdssg qlvpvpvdqa ghfekdassq
      721 teepcdnles lkselnnaka qvkflqkslk dsfsvkvtes ptgpnaqpli eidqnlpvlg
      781 slgsqsprks glsanrrwrk qltnqkmsef glsenvfelq eeifflvlri edlerqlkdn
      841 fevvskhkde slseqagvav vqaleeknaa lqeqlhqmqe vstvllcrle elaafleqll
      901 aydetgslgs vtlsadmvak irqlvadskd fslsfsqsfl gsdnnsalsd hllkrgsfdr
      961 smqsilsrsk gkndsisvid kpqsckldin tippcpedia fmedelktlr drvqeqqsei
     1021 ssltrrleee gseraspaag grcpsrrhpg tpaslceged tdaswryqdq vvlvqasdsd
     1081 dillllnerg lpenvylrst wqkqalnaae slpavcsava aeeadadady ssssspsllr
     1141 wkearnsale ngapqeplvp igrfqcwssd sdiwsepdrs vseqrmggvc rkewnkqprr
     1201 spketrkama ggrqeqpgsd tgakqakdsw pevakgkrls cptdggrskr avsashtkaq
     1261 klekllrriq hcnsrledtl tvhkelcqkl lripsgdgpk dasesqqpkd lteslvpsmr
     1321 klqfrleemv innevlqeil tehvasgkav ltepctpgga ganskegaae elnqckkela
     1381 ckerredilk ramvheqdak vqlekeladc qkelqslhea hcalekqykt avqrnsdlal
     1441 rdpqrrssfe ksrlerargs sferpdgeem klrdenhtlt aelrqqaqei arlgkeealh
     1501 kdevsnlsqe ktvlsqklqa vqhehevlle sssnkiselv tklgalstqn knqheeirnl
     1561 remgesisre niklkhdlda tsseleglrv enqdlretll alrkqesllr knlekavdeq
     1621 qaakkisdey sqsnkllrme kdqlgakisd lssrldalec ekqslyeekq rlilevsage
     1681 devervnskl qsceeecnnl rtqkseveae lqacnnklqe fehlaeqlre elsatkgkle
     1741 smdtrcnhle qelgaskeah kqllwkrdel qrtleavgre aqqskslarm sldkkhvmaf
     1801 melqdksrsl qnvvmelklk leramyenkc lrlelrvneq fqsgnssgav vgtpdslrsf
     1861 ataasnaspp rlsphqvpvd ssqrtppvvg qhscshsrsy htspdlgids dptpehdvys
     1921 taveptyveh wqhrmgpdws issvhsapda fggscllcqq gkdspaswkf kanatyggeq
     1981 ldhpstqsps ksgfpviscs lsspgvhlca ivqlsdhell kkrvdesicl irrveasvqq
     2041 aldaladfas dcteppecnr llqdaergcq sikmclmdsf klicsftmaq mpeakeehsf
     2101 ehkqlrmeln klqekhsqqs aelqnaldqi sslkekkegm eraisrqlnk tkmvlkqtkg
     2161 nlnvlrarda kpekk
//
LOCUS       XP_077505199            2115 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114981 isoform X12 [Amblyomma
            americanum].
ACCESSION   XP_077505199
VERSION     XP_077505199.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649073.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2115
                     /product="uncharacterized protein LOC144114981 isoform
                     X12"
                     /calculated_mol_wt=236885
     Region          <93..>365
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <1310..>1599
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          <1484..1782
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..2115
                     /gene="LOC144114981"
                     /coded_by="XM_077649073.1:153..6500"
                     /db_xref="GeneID:144114981"
ORIGIN      
        1 mdnpqsness aaesrpetnl dlpassmeap tsgshgavan qehslrnyeq niislrqenl
       61 rlrlrsfylq lqtktpaeen enefdvaify hqknvaeqiq kfvtelderd klldesrtkr
      121 eeleklvanl ihekaalede lckwkmktnv seaaqddnvk mvstlshvkk eledkvkqle
      181 qqldelkdkk vpslkrqldt rtaalqaisa haakqeealk slhnvhslcs liqpkteafl
      241 qliddslaag diqatqrakn dfqrelskis sehcsfqlds teqpcsvaaf sdrvwkleae
      301 lqsrenliec lnsdiaclts rleatqtrcl eyetemkras eihqkicaek dklldsvqrk
      361 mqemerhaqe glspksrtse lqlecnrlhi avleksrfin ellsernkat veteksfhel
      421 llsmkqkdql iemmqtkfeh aeegeavien eglrqpnpad flletcegqp kpaepgtplg
      481 terpsfqgss paspegpdep tvfylsqsvp revkvlyqil sdlaqesgim pelstvaltn
      541 isciqkvtei lksahekglr fslpiqstiv nmdsldhcep derglaawep tvlvklgekl
      601 qqlaylfavd gpvvskwqma dvvqlldtvl ekakqeaank lflhrspedl ppvlfgkggq
      661 pkeheelths amlsphlrne rghsdisdyq eellqdlgsa lkcvgdlhne vkalpltpts
      721 netrkstgst delifavdss gqlvpvpvdq aghfekdass qteepcdnle slkselnnak
      781 aqvvskhkde slseqagvav vqaleeknaa lqeqlhqmqe vstvllcrle elaafleqll
      841 aydetgslgs vtlsadmvak irqlvadskd fslsfsqsfl gsdnnsalsd hllkrgsfdr
      901 smqsilsrsk gkndsisvid kpqsckldin tippcpedia fmedelktlr drvqeqqsei
      961 ssltrrleee gseraspaag grcpsrrhpg tpaslceged tdaswryqdq vvlvqasdsd
     1021 dillllnerg lpenvylrst wqkqalnaae slpavcsava aeeadadady ssssspsllr
     1081 wkearnsale ngapqeplvp igrfqcwssd sdiwsepdrs vseqrmggvc rkewnkqprr
     1141 spketrkama ggrqeqpgsd tgakqakdsw pevakgkrls cptdggrskr avsashtkaq
     1201 klekllrriq hcnsrledtl tvhkelcqkl lripsgdgpk dasesqqpkd lteslvpsmr
     1261 klqfrleemv innevlqeil tehvasgkav ltepctpgga ganskegaae elnqckkela
     1321 ckerredilk ramvheqdak vqlekeladc qkelqslhea hcalekqykt avqrnsdlal
     1381 rdpqrrssfe ksrlerargs sferpdgeem klrdenhtlt aelrqqaqei arlgkeealh
     1441 kdevsnlsqe ktvlsqklqa vqhehevlle sssnkiselv tklgalstqn knqheeirnl
     1501 remgesisre niklkhdlda tsseleglrv enqdlretll alrkqesllr knlekavdeq
     1561 qaakkisdey sqsnkllrme kdqlgakisd lssrldalec ekqslyeekq rlilevsage
     1621 devervnskl qsceeecnnl rtqkseveae lqacnnklqe fehlaeqlre elsatkgkle
     1681 smdtrcnhle qelgaskeah kqllwkrdel qrtleavgre aqqskslarm sldkkhvmaf
     1741 melqdksrsl qnvvmelklk leramyenkc lrlelrvneq fqsgnssgav vgtpdslrsf
     1801 ataasnaspp rlsphqvpvd ssqrtppvvg qhscshsrsy htspdlgids dptpehdvys
     1861 taveptyveh wqhrmgpdws issvhsapda fggscllcqq gkdspaswkf kanatyggeq
     1921 ldhpstqsps ksgfpviscs lsspgvhlca ivqlsdhell kkrvdesicl irrveasvqq
     1981 aldaladfas dcteppecnr llqdaergcq sikmclmdsf klicsftmaq mpeakeehsf
     2041 ehkqlrmeln klqekhsqqs aelqnaldqi sslkekkegm eraisrqlnk tkmvlkqtkg
     2101 nlnvlrarda kpekk
//
LOCUS       XP_077505200            2089 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114981 isoform X13 [Amblyomma
            americanum].
ACCESSION   XP_077505200
VERSION     XP_077505200.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649074.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2089
                     /product="uncharacterized protein LOC144114981 isoform
                     X13"
                     /calculated_mol_wt=234081
     Region          <93..>365
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <1284..>1573
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          <1458..1756
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..2089
                     /gene="LOC144114981"
                     /coded_by="XM_077649074.1:153..6422"
                     /db_xref="GeneID:144114981"
ORIGIN      
        1 mdnpqsness aaesrpetnl dlpassmeap tsgshgavan qehslrnyeq niislrqenl
       61 rlrlrsfylq lqtktpaeen enefdvaify hqknvaeqiq kfvtelderd klldesrtkr
      121 eeleklvanl ihekaalede lckwkmktnv seaaqddnvk mvstlshvkk eledkvkqle
      181 qqldelkdkk vpslkrqldt rtaalqaisa haakqeealk slhnvhslcs liqpkteafl
      241 qliddslaag diqatqrakn dfqrelskis sehcsfqlds teqpcsvaaf sdrvwkleae
      301 lqsrenliec lnsdiaclts rleatqtrcl eyetemkras eihqkicaek dklldsvqrk
      361 mqemerhaqe glspksrtse lqlecnrlhi avleksrfin ellsernkat veteksfhel
      421 llsmkqkdql iemmqtkfeh aeegeavien eglrqpnpad flletcegqp kpaepgtplg
      481 terpsfqgss paspegpdep tvfylsqsvp revkvlyqil sdlaqesgim pelstvaltn
      541 isciqkvtei lksahekglr fslpiqstiv nmdsldhcep derglaawep tvlvklgekl
      601 qqlaylfavd gpvvskwqma dvvqlldtvl ekakqeaank lflhrspedl ppvlfgkggq
      661 pkeheelths amlsphlrne rghsdisdyq eellqdlgsa lkcvgdlhne vkalpltpts
      721 netrkstgst delifavdss gqlvpvpvdq aghfekvvsk hkdeslseqa gvavvqalee
      781 knaalqeqlh qmqevstvll crleelaafl eqllaydetg slgsvtlsad mvakirqlva
      841 dskdfslsfs qsflgsdnns alsdhllkrg sfdrsmqsil srskgkndsi svidkpqsck
      901 ldintippcp ediafmedel ktlrdrvqeq qseissltrr leeegseras paaggrcpsr
      961 rhpgtpaslc egedtdaswr yqdqvvlvqa sdsddillll nerglpenvy lrstwqkqal
     1021 naaeslpavc savaaeeada dadysssssp sllrwkearn salengapqe plvpigrfqc
     1081 wssdsdiwse pdrsvseqrm ggvcrkewnk qprrspketr kamaggrqeq pgsdtgakqa
     1141 kdswpevakg krlscptdgg rskravsash tkaqklekll rriqhcnsrl edtltvhkel
     1201 cqkllripsg dgpkdasesq qpkdlteslv psmrklqfrl eemvinnevl qeiltehvas
     1261 gkavltepct pggaganske gaaeelnqck kelackerre dilkramvhe qdakvqleke
     1321 ladcqkelqs lheahcalek qyktavqrns dlalrdpqrr ssfeksrler argssferpd
     1381 geemklrden htltaelrqq aqeiarlgke ealhkdevsn lsqektvlsq klqavqhehe
     1441 vllesssnki selvtklgal stqnknqhee irnlremges isreniklkh dldatssele
     1501 glrvenqdlr etllalrkqe sllrknleka vdeqqaakki sdeysqsnkl lrmekdqlga
     1561 kisdlssrld alecekqsly eekqrlilev sagedeverv nsklqsceee cnnlrtqkse
     1621 veaelqacnn klqefehlae qlreelsatk gklesmdtrc nhleqelgas keahkqllwk
     1681 rdelqrtlea vgreaqqsks larmsldkkh vmafmelqdk srslqnvvme lklkleramy
     1741 enkclrlelr vneqfqsgns sgavvgtpds lrsfataasn aspprlsphq vpvdssqrtp
     1801 pvvgqhscsh srsyhtspdl gidsdptpeh dvystavept yvehwqhrmg pdwsissvhs
     1861 apdafggscl lcqqgkdspa swkfkanaty ggeqldhpst qspsksgfpv iscslsspgv
     1921 hlcaivqlsd hellkkrvde siclirrvea svqqaldala dfasdctepp ecnrllqdae
     1981 rgcqsikmcl mdsfklicsf tmaqmpeake ehsfehkqlr melnklqekh sqqsaelqna
     2041 ldqisslkek kegmeraisr qlnktkmvlk qtkgnlnvlr ardakpekk
//
LOCUS       XP_077505201            2011 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114981 isoform X14 [Amblyomma
            americanum].
ACCESSION   XP_077505201
VERSION     XP_077505201.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649075.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2011
                     /product="uncharacterized protein LOC144114981 isoform
                     X14"
                     /calculated_mol_wt=225993
     Region          <93..>365
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <870..>1484
                     /region_name="CCDC158"
                     /note="Coiled-coil domain-containing protein 158;
                     pfam15921"
                     /db_xref="CDD:464943"
     Region          <1206..>1495
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          <1380..1678
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..2011
                     /gene="LOC144114981"
                     /coded_by="XM_077649075.1:153..6188"
                     /db_xref="GeneID:144114981"
ORIGIN      
        1 mdnpqsness aaesrpetnl dlpassmeap tsgshgavan qehslrnyeq niislrqenl
       61 rlrlrsfylq lqtktpaeen enefdvaify hqknvaeqiq kfvtelderd klldesrtkr
      121 eeleklvanl ihekaalede lckwkmktnv seaaqddnvk mvstlshvkk eledkvkqle
      181 qqldelkdkk vpslkrqldt rtaalqaisa haakqeealk slhnvhslcs liqpkteafl
      241 qliddslaag diqatqrakn dfqrelskis sehcsfqlds teqpcsvaaf sdrvwkleae
      301 lqsrenliec lnsdiaclts rleatqtrcl eyetemkras eihqkicaek dklldsvqrk
      361 mqemerhaqe glspksrtse lqlecnrlhi avleksrfin ellsernkat veteksfhel
      421 llsmkqkdql iemmqtkfeh aeegeavien eglrqpnpad flletcegqp kpaepgtplg
      481 terpsfqgss paspegpdep tvfylsqsvp revkvlyqil sdlaqesgim pelstvaltn
      541 isciqkvtei lksahekglr fslpiqstiv nmdsldhcep derglaawep tvlvklgekl
      601 qqlaylfavd gpvvskwqma dvvqlldtvl ekakqeaank lflhrspedl ppvlfgkggq
      661 pkeheelths amlsphlrne rghsdisdyq eellqdlgsa lkcvgdlhne vkalpltpts
      721 netrkstgst delifavdss gqlvpvpvdq aghfekdass qteepcdnle slkselnnak
      781 aqvkflqksl kdsfsvkvte sptgpnaqpl ieidqnlpvl gslgsqsprk sglsanrrwr
      841 kqltnqkmse fglsenvfel qeeifflvlr iedlerqlkd nfevvskhkd eslseqagva
      901 vvqaleekna alqeqlhqmq evstvllcrl eelaafleql laydetgslg svtlsadmva
      961 kirqlvadsk dfslsfsqsf lgsdnnsals dhllkrgsfd rsmqsilsrs kgkndsisvi
     1021 dkpqsckldi ntippcpedi afmedelktl rdrvqeqqse issltrrlee egseraspaa
     1081 ggrskravsa shtkaqklek llrriqhcns rledtltvhk elcqkllrip sgdgpkdase
     1141 sqqpkdltes lvpsmrklqf rleemvinne vlqeiltehv asgkavltep ctpggagans
     1201 kegaaeelnq ckkelacker redilkramv heqdakvqle keladcqkel qslheahcal
     1261 ekqyktavqr nsdlalrdpq rrssfeksrl erargssfer pdgeemklrd enhtltaelr
     1321 qqaqeiarlg keealhkdev snlsqektvl sqklqavqhe hevllesssn kiselvtklg
     1381 alstqnknqh eeirnlremg esisrenikl khdldatsse leglrvenqd lretllalrk
     1441 qesllrknle kavdeqqaak kisdeysqsn kllrmekdql gakisdlssr ldalecekqs
     1501 lyeekqrlil evsagedeve rvnsklqsce eecnnlrtqk seveaelqac nnklqefehl
     1561 aeqlreelsa tkgklesmdt rcnhleqelg askeahkqll wkrdelqrtl eavgreaqqs
     1621 kslarmsldk khvmafmelq dksrslqnvv melklklera myenkclrle lrvneqfqsg
     1681 nssgavvgtp dslrsfataa snaspprlsp hqvpvdssqr tppvvgqhsc shsrsyhtsp
     1741 dlgidsdptp ehdvystave ptyvehwqhr mgpdwsissv hsapdafggs cllcqqgkds
     1801 paswkfkana tyggeqldhp stqspsksgf pviscslssp gvhlcaivql sdhellkkrv
     1861 desiclirrv easvqqalda ladfasdcte ppecnrllqd aergcqsikm clmdsfklic
     1921 sftmaqmpea keehsfehkq lrmelnklqe khsqqsaelq naldqisslk ekkegmerai
     1981 srqlnktkmv lkqtkgnlnv lrardakpek k
//
LOCUS       XP_077505203            2004 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114981 isoform X15 [Amblyomma
            americanum].
ACCESSION   XP_077505203
VERSION     XP_077505203.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649077.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2004
                     /product="uncharacterized protein LOC144114981 isoform
                     X15"
                     /calculated_mol_wt=225147
     Region          <86..>358
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <863..>1477
                     /region_name="CCDC158"
                     /note="Coiled-coil domain-containing protein 158;
                     pfam15921"
                     /db_xref="CDD:464943"
     Region          <1199..>1488
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          <1373..1671
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..2004
                     /gene="LOC144114981"
                     /coded_by="XM_077649077.1:153..6167"
                     /db_xref="GeneID:144114981"
ORIGIN      
        1 mdnpqsness aaesrpetnl dlpassmeap tsgshgavan qehslrnyeq niislrqenl
       61 rlrlrsfylq lqtktpaeen enefqknvae qiqkfvteld erdklldesr tkreeleklv
      121 anlihekaal edelckwkmk tnvseaaqdd nvkmvstlsh vkkeledkvk qleqqldelk
      181 dkkvpslkrq ldtrtaalqa isahaakqee alkslhnvhs lcsliqpkte aflqliddsl
      241 aagdiqatqr akndfqrels kissehcsfq ldsteqpcsv aafsdrvwkl eaelqsrenl
      301 ieclnsdiac ltsrleatqt rcleyetemk raseihqkic aekdklldsv qrkmqemerh
      361 aqeglspksr tselqlecnr lhiavleksr finellsern katveteksf helllsmkqk
      421 dqliemmqtk fehaeegeav ieneglrqpn padflletce gqpkpaepgt plgterpsfq
      481 gsspaspegp deptvfylsq svprevkvly qilsdlaqes gimpelstva ltnisciqkv
      541 teilksahek glrfslpiqs tivnmdsldh cepderglaa weptvlvklg eklqqlaylf
      601 avdgpvvskw qmadvvqlld tvlekakqea anklflhrsp edlppvlfgk ggqpkeheel
      661 thsamlsphl rnerghsdis dyqeellqdl gsalkcvgdl hnevkalplt ptsnetrkst
      721 gstdelifav dssgqlvpvp vdqaghfekd assqteepcd nleslkseln nakaqvkflq
      781 kslkdsfsvk vtesptgpna qplieidqnl pvlgslgsqs prksglsanr rwrkqltnqk
      841 msefglsenv felqeeiffl vlriedlerq lkdnfevvsk hkdeslseqa gvavvqalee
      901 knaalqeqlh qmqevstvll crleelaafl eqllaydetg slgsvtlsad mvakirqlva
      961 dskdfslsfs qsflgsdnns alsdhllkrg sfdrsmqsil srskgkndsi svidkpqsck
     1021 ldintippcp ediafmedel ktlrdrvqeq qseissltrr leeegseras paaggrskra
     1081 vsashtkaqk lekllrriqh cnsrledtlt vhkelcqkll ripsgdgpkd asesqqpkdl
     1141 teslvpsmrk lqfrleemvi nnevlqeilt ehvasgkavl tepctpggag anskegaaee
     1201 lnqckkelac kerredilkr amvheqdakv qlekeladcq kelqslheah calekqykta
     1261 vqrnsdlalr dpqrrssfek srlerargss ferpdgeemk lrdenhtlta elrqqaqeia
     1321 rlgkeealhk devsnlsqek tvlsqklqav qhehevlles ssnkiselvt klgalstqnk
     1381 nqheeirnlr emgesisren iklkhdldat sseleglrve nqdlretlla lrkqesllrk
     1441 nlekavdeqq aakkisdeys qsnkllrmek dqlgakisdl ssrldalece kqslyeekqr
     1501 lilevsaged evervnsklq sceeecnnlr tqkseveael qacnnklqef ehlaeqlree
     1561 lsatkgkles mdtrcnhleq elgaskeahk qllwkrdelq rtleavgrea qqskslarms
     1621 ldkkhvmafm elqdksrslq nvvmelklkl eramyenkcl rlelrvneqf qsgnssgavv
     1681 gtpdslrsfa taasnasppr lsphqvpvds sqrtppvvgq hscshsrsyh tspdlgidsd
     1741 ptpehdvyst aveptyvehw qhrmgpdwsi ssvhsapdaf ggscllcqqg kdspaswkfk
     1801 anatyggeql dhpstqspsk sgfpviscsl sspgvhlcai vqlsdhellk krvdesicli
     1861 rrveasvqqa ldaladfasd cteppecnrl lqdaergcqs ikmclmdsfk licsftmaqm
     1921 peakeehsfe hkqlrmelnk lqekhsqqsa elqnaldqis slkekkegme raisrqlnkt
     1981 kmvlkqtkgn lnvlrardak pekk
//
LOCUS       XP_077505204            2003 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114981 isoform X16 [Amblyomma
            americanum].
ACCESSION   XP_077505204
VERSION     XP_077505204.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649078.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2003
                     /product="uncharacterized protein LOC144114981 isoform
                     X16"
                     /calculated_mol_wt=225019
     Region          <71..>357
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <862..>1476
                     /region_name="CCDC158"
                     /note="Coiled-coil domain-containing protein 158;
                     pfam15921"
                     /db_xref="CDD:464943"
     Region          <1198..>1487
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          <1372..1670
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..2003
                     /gene="LOC144114981"
                     /coded_by="XM_077649078.1:153..6164"
                     /db_xref="GeneID:144114981"
ORIGIN      
        1 mdnpqsness aaesrpetnl dlpassmeap tsgshgavan qehslrnyeq niislrqenl
       61 rlrlrsfylq lqtktpaeen enefknvaeq iqkfvtelde rdklldesrt kreeleklva
      121 nlihekaale delckwkmkt nvseaaqddn vkmvstlshv kkeledkvkq leqqldelkd
      181 kkvpslkrql dtrtaalqai sahaakqeea lkslhnvhsl csliqpktea flqliddsla
      241 agdiqatqra kndfqrelsk issehcsfql dsteqpcsva afsdrvwkle aelqsrenli
      301 eclnsdiacl tsrleatqtr cleyetemkr aseihqkica ekdklldsvq rkmqemerha
      361 qeglspksrt selqlecnrl hiavleksrf inellsernk atveteksfh elllsmkqkd
      421 qliemmqtkf ehaeegeavi eneglrqpnp adflletceg qpkpaepgtp lgterpsfqg
      481 sspaspegpd eptvfylsqs vprevkvlyq ilsdlaqesg impelstval tnisciqkvt
      541 eilksahekg lrfslpiqst ivnmdsldhc epderglaaw eptvlvklge klqqlaylfa
      601 vdgpvvskwq madvvqlldt vlekakqeaa nklflhrspe dlppvlfgkg gqpkeheelt
      661 hsamlsphlr nerghsdisd yqeellqdlg salkcvgdlh nevkalpltp tsnetrkstg
      721 stdelifavd ssgqlvpvpv dqaghfekda ssqteepcdn leslkselnn akaqvkflqk
      781 slkdsfsvkv tesptgpnaq plieidqnlp vlgslgsqsp rksglsanrr wrkqltnqkm
      841 sefglsenvf elqeeifflv lriedlerql kdnfevvskh kdeslseqag vavvqaleek
      901 naalqeqlhq mqevstvllc rleelaafle qllaydetgs lgsvtlsadm vakirqlvad
      961 skdfslsfsq sflgsdnnsa lsdhllkrgs fdrsmqsils rskgkndsis vidkpqsckl
     1021 dintippcpe diafmedelk tlrdrvqeqq seissltrrl eeegserasp aaggrskrav
     1081 sashtkaqkl ekllrriqhc nsrledtltv hkelcqkllr ipsgdgpkda sesqqpkdlt
     1141 eslvpsmrkl qfrleemvin nevlqeilte hvasgkavlt epctpggaga nskegaaeel
     1201 nqckkelack erredilkra mvheqdakvq lekeladcqk elqslheahc alekqyktav
     1261 qrnsdlalrd pqrrssfeks rlerargssf erpdgeemkl rdenhtltae lrqqaqeiar
     1321 lgkeealhkd evsnlsqekt vlsqklqavq hehevlless snkiselvtk lgalstqnkn
     1381 qheeirnlre mgesisreni klkhdldats seleglrven qdlretllal rkqesllrkn
     1441 lekavdeqqa akkisdeysq snkllrmekd qlgakisdls srldalecek qslyeekqrl
     1501 ilevsagede vervnsklqs ceeecnnlrt qkseveaelq acnnklqefe hlaeqlreel
     1561 satkgklesm dtrcnhleqe lgaskeahkq llwkrdelqr tleavgreaq qskslarmsl
     1621 dkkhvmafme lqdksrslqn vvmelklkle ramyenkclr lelrvneqfq sgnssgavvg
     1681 tpdslrsfat aasnaspprl sphqvpvdss qrtppvvgqh scshsrsyht spdlgidsdp
     1741 tpehdvysta veptyvehwq hrmgpdwsis svhsapdafg gscllcqqgk dspaswkfka
     1801 natyggeqld hpstqspsks gfpviscsls spgvhlcaiv qlsdhellkk rvdesiclir
     1861 rveasvqqal daladfasdc teppecnrll qdaergcqsi kmclmdsfkl icsftmaqmp
     1921 eakeehsfeh kqlrmelnkl qekhsqqsae lqnaldqiss lkekkegmer aisrqlnktk
     1981 mvlkqtkgnl nvlrardakp ekk
//
LOCUS       XP_077505205            1983 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114981 isoform X17 [Amblyomma
            americanum].
ACCESSION   XP_077505205
VERSION     XP_077505205.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649079.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1983
                     /product="uncharacterized protein LOC144114981 isoform
                     X17"
                     /calculated_mol_wt=222504
     Region          <36..>337
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <842..>1456
                     /region_name="CCDC158"
                     /note="Coiled-coil domain-containing protein 158;
                     pfam15921"
                     /db_xref="CDD:464943"
     Region          <1178..>1467
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          <1352..1650
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1983
                     /gene="LOC144114981"
                     /coded_by="XM_077649079.1:153..6104"
                     /db_xref="GeneID:144114981"
ORIGIN      
        1 mdnpqsness aaesrpetnl dlpassmeap tsgshgavan qehslrnyeq lqtktpaeen
       61 enefknvaeq iqkfvtelde rdklldesrt kreeleklva nlihekaale delckwkmkt
      121 nvseaaqddn vkmvstlshv kkeledkvkq leqqldelkd kkvpslkrql dtrtaalqai
      181 sahaakqeea lkslhnvhsl csliqpktea flqliddsla agdiqatqra kndfqrelsk
      241 issehcsfql dsteqpcsva afsdrvwkle aelqsrenli eclnsdiacl tsrleatqtr
      301 cleyetemkr aseihqkica ekdklldsvq rkmqemerha qeglspksrt selqlecnrl
      361 hiavleksrf inellsernk atveteksfh elllsmkqkd qliemmqtkf ehaeegeavi
      421 eneglrqpnp adflletceg qpkpaepgtp lgterpsfqg sspaspegpd eptvfylsqs
      481 vprevkvlyq ilsdlaqesg impelstval tnisciqkvt eilksahekg lrfslpiqst
      541 ivnmdsldhc epderglaaw eptvlvklge klqqlaylfa vdgpvvskwq madvvqlldt
      601 vlekakqeaa nklflhrspe dlppvlfgkg gqpkeheelt hsamlsphlr nerghsdisd
      661 yqeellqdlg salkcvgdlh nevkalpltp tsnetrkstg stdelifavd ssgqlvpvpv
      721 dqaghfekda ssqteepcdn leslkselnn akaqvkflqk slkdsfsvkv tesptgpnaq
      781 plieidqnlp vlgslgsqsp rksglsanrr wrkqltnqkm sefglsenvf elqeeifflv
      841 lriedlerql kdnfevvskh kdeslseqag vavvqaleek naalqeqlhq mqevstvllc
      901 rleelaafle qllaydetgs lgsvtlsadm vakirqlvad skdfslsfsq sflgsdnnsa
      961 lsdhllkrgs fdrsmqsils rskgkndsis vidkpqsckl dintippcpe diafmedelk
     1021 tlrdrvqeqq seissltrrl eeegserasp aaggrskrav sashtkaqkl ekllrriqhc
     1081 nsrledtltv hkelcqkllr ipsgdgpkda sesqqpkdlt eslvpsmrkl qfrleemvin
     1141 nevlqeilte hvasgkavlt epctpggaga nskegaaeel nqckkelack erredilkra
     1201 mvheqdakvq lekeladcqk elqslheahc alekqyktav qrnsdlalrd pqrrssfeks
     1261 rlerargssf erpdgeemkl rdenhtltae lrqqaqeiar lgkeealhkd evsnlsqekt
     1321 vlsqklqavq hehevlless snkiselvtk lgalstqnkn qheeirnlre mgesisreni
     1381 klkhdldats seleglrven qdlretllal rkqesllrkn lekavdeqqa akkisdeysq
     1441 snkllrmekd qlgakisdls srldalecek qslyeekqrl ilevsagede vervnsklqs
     1501 ceeecnnlrt qkseveaelq acnnklqefe hlaeqlreel satkgklesm dtrcnhleqe
     1561 lgaskeahkq llwkrdelqr tleavgreaq qskslarmsl dkkhvmafme lqdksrslqn
     1621 vvmelklkle ramyenkclr lelrvneqfq sgnssgavvg tpdslrsfat aasnaspprl
     1681 sphqvpvdss qrtppvvgqh scshsrsyht spdlgidsdp tpehdvysta veptyvehwq
     1741 hrmgpdwsis svhsapdafg gscllcqqgk dspaswkfka natyggeqld hpstqspsks
     1801 gfpviscsls spgvhlcaiv qlsdhellkk rvdesiclir rveasvqqal daladfasdc
     1861 teppecnrll qdaergcqsi kmclmdsfkl icsftmaqmp eakeehsfeh kqlrmelnkl
     1921 qekhsqqsae lqnaldqiss lkekkegmer aisrqlnktk mvlkqtkgnl nvlrardakp
     1981 ekk
//
LOCUS       XP_077505206            1977 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114981 isoform X18 [Amblyomma
            americanum].
ACCESSION   XP_077505206
VERSION     XP_077505206.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649080.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1977
                     /product="uncharacterized protein LOC144114981 isoform
                     X18"
                     /calculated_mol_wt=222289
     Region          <45..>331
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <836..>1450
                     /region_name="CCDC158"
                     /note="Coiled-coil domain-containing protein 158;
                     pfam15921"
                     /db_xref="CDD:464943"
     Region          <1172..>1461
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          <1346..1644
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1977
                     /gene="LOC144114981"
                     /coded_by="XM_077649080.1:219..6152"
                     /db_xref="GeneID:144114981"
ORIGIN      
        1 meaptsgshg avanqehslr nyeqniislr qenlrlrlrs fylqlqtktp aeenenefkn
       61 vaeqiqkfvt elderdklld esrtkreele klvanlihek aaledelckw kmktnvseaa
      121 qddnvkmvst lshvkkeled kvkqleqqld elkdkkvpsl krqldtrtaa lqaisahaak
      181 qeealkslhn vhslcsliqp kteaflqlid dslaagdiqa tqrakndfqr elskissehc
      241 sfqldsteqp csvaafsdrv wkleaelqsr enlieclnsd iacltsrlea tqtrcleyet
      301 emkraseihq kicaekdkll dsvqrkmqem erhaqeglsp ksrtselqle cnrlhiavle
      361 ksrfinells ernkatvete ksfhelllsm kqkdqliemm qtkfehaeeg eavieneglr
      421 qpnpadflle tcegqpkpae pgtplgterp sfqgsspasp egpdeptvfy lsqsvprevk
      481 vlyqilsdla qesgimpels tvaltnisci qkvteilksa hekglrfslp iqstivnmds
      541 ldhcepderg laaweptvlv klgeklqqla ylfavdgpvv skwqmadvvq lldtvlekak
      601 qeaanklflh rspedlppvl fgkggqpkeh eelthsamls phlrnerghs disdyqeell
      661 qdlgsalkcv gdlhnevkal pltptsnetr kstgstdeli favdssgqlv pvpvdqaghf
      721 ekdassqtee pcdnleslks elnnakaqvk flqkslkdsf svkvtesptg pnaqplieid
      781 qnlpvlgslg sqsprksgls anrrwrkqlt nqkmsefgls envfelqeei fflvlriedl
      841 erqlkdnfev vskhkdesls eqagvavvqa leeknaalqe qlhqmqevst vllcrleela
      901 afleqllayd etgslgsvtl sadmvakirq lvadskdfsl sfsqsflgsd nnsalsdhll
      961 krgsfdrsmq silsrskgkn dsisvidkpq sckldintip pcpediafme delktlrdrv
     1021 qeqqseissl trrleeegse raspaaggrs kravsashtk aqklekllrr iqhcnsrled
     1081 tltvhkelcq kllripsgdg pkdasesqqp kdlteslvps mrklqfrlee mvinnevlqe
     1141 iltehvasgk avltepctpg gaganskega aeelnqckke lackerredi lkramvheqd
     1201 akvqlekela dcqkelqslh eahcalekqy ktavqrnsdl alrdpqrrss feksrlerar
     1261 gssferpdge emklrdenht ltaelrqqaq eiarlgkeea lhkdevsnls qektvlsqkl
     1321 qavqhehevl lesssnkise lvtklgalst qnknqheeir nlremgesis reniklkhdl
     1381 datsselegl rvenqdlret llalrkqesl lrknlekavd eqqaakkisd eysqsnkllr
     1441 mekdqlgaki sdlssrldal ecekqslyee kqrlilevsa gedevervns klqsceeecn
     1501 nlrtqkseve aelqacnnkl qefehlaeql reelsatkgk lesmdtrcnh leqelgaske
     1561 ahkqllwkrd elqrtleavg reaqqsksla rmsldkkhvm afmelqdksr slqnvvmelk
     1621 lkleramyen kclrlelrvn eqfqsgnssg avvgtpdslr sfataasnas pprlsphqvp
     1681 vdssqrtppv vgqhscshsr syhtspdlgi dsdptpehdv ystaveptyv ehwqhrmgpd
     1741 wsissvhsap dafggscllc qqgkdspasw kfkanatygg eqldhpstqs psksgfpvis
     1801 cslsspgvhl caivqlsdhe llkkrvdesi clirrveasv qqaldaladf asdcteppec
     1861 nrllqdaerg cqsikmclmd sfklicsftm aqmpeakeeh sfehkqlrme lnklqekhsq
     1921 qsaelqnald qisslkekke gmeraisrql nktkmvlkqt kgnlnvlrar dakpekk
//
LOCUS       XP_077505207            1903 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114981 isoform X19 [Amblyomma
            americanum].
ACCESSION   XP_077505207
VERSION     XP_077505207.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649081.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1903
                     /product="uncharacterized protein LOC144114981 isoform
                     X19"
                     /calculated_mol_wt=213569
     Region          <86..>358
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <760..>1376
                     /region_name="CCDC158"
                     /note="Coiled-coil domain-containing protein 158;
                     pfam15921"
                     /db_xref="CDD:464943"
     Region          <1098..>1387
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          <1272..1570
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1903
                     /gene="LOC144114981"
                     /coded_by="XM_077649081.1:153..5864"
                     /db_xref="GeneID:144114981"
ORIGIN      
        1 mdnpqsness aaesrpetnl dlpassmeap tsgshgavan qehslrnyeq niislrqenl
       61 rlrlrsfylq lqtktpaeen enefqknvae qiqkfvteld erdklldesr tkreeleklv
      121 anlihekaal edelckwkmk tnvseaaqdd nvkmvstlsh vkkeledkvk qleqqldelk
      181 dkkvpslkrq ldtrtaalqa isahaakqee alkslhnvhs lcsliqpkte aflqliddsl
      241 aagdiqatqr akndfqrels kissehcsfq ldsteqpcsv aafsdrvwkl eaelqsrenl
      301 ieclnsdiac ltsrleatqt rcleyetemk raseihqkic aekdklldsv qrkmqemerh
      361 aqeglspksr tselqlecnr lhiavleksr finellsern katveteksf helllsmkqk
      421 dqliemmqtk fehaeegeav ieneglrqpn padflletce gqpkpaepgt plgterpsfq
      481 gsspaspegp deptvfylsq svprevkvly qilsdlaqes gimpelstva ltnisciqkv
      541 teilksahek glrfslpiqs tivnmdsldh cepderglaa weptvlvklg eklqqlaylf
      601 avdgpvvskw qmadvvqlld tvlekakqea anklflhrsp edlppvlfgk ggqpkeheel
      661 thsamlsphl rnerghsdis dyqeellqdl gsalkcvgdl hnevkalplt ptsnetrkst
      721 gstdelifav dssgqlvpvp vdqaghfekd assqteepcd nleslkseln nakaqvvskh
      781 kdeslseqag vavvqaleek naalqeqlhq mqevstvllc rleelaafle qllaydetgs
      841 lgsvtlsadm vakirqlvad skdfslsfsq sflgsdnnsa lsdhllkrgs fdrsmqsils
      901 rskgkndsis vidkpqsckl dintippcpe diafmedelk tlrdrvqeqq seissltrrl
      961 eeegserasp aaggrskrav sashtkaqkl ekllrriqhc nsrledtltv hkelcqkllr
     1021 ipsgdgpkda sesqqpkdlt eslvpsmrkl qfrleemvin nevlqeilte hvasgkavlt
     1081 epctpggaga nskegaaeel nqckkelack erredilkra mvheqdakvq lekeladcqk
     1141 elqslheahc alekqyktav qrnsdlalrd pqrrssfeks rlerargssf erpdgeemkl
     1201 rdenhtltae lrqqaqeiar lgkeealhkd evsnlsqekt vlsqklqavq hehevlless
     1261 snkiselvtk lgalstqnkn qheeirnlre mgesisreni klkhdldats seleglrven
     1321 qdlretllal rkqesllrkn lekavdeqqa akkisdeysq snkllrmekd qlgakisdls
     1381 srldalecek qslyeekqrl ilevsagede vervnsklqs ceeecnnlrt qkseveaelq
     1441 acnnklqefe hlaeqlreel satkgklesm dtrcnhleqe lgaskeahkq llwkrdelqr
     1501 tleavgreaq qskslarmsl dkkhvmafme lqdksrslqn vvmelklkle ramyenkclr
     1561 lelrvneqfq sgnssgavvg tpdslrsfat aasnaspprl sphqvpvdss qrtppvvgqh
     1621 scshsrsyht spdlgidsdp tpehdvysta veptyvehwq hrmgpdwsis svhsapdafg
     1681 gscllcqqgk dspaswkfka natyggeqld hpstqspsks gfpviscsls spgvhlcaiv
     1741 qlsdhellkk rvdesiclir rveasvqqal daladfasdc teppecnrll qdaergcqsi
     1801 kmclmdsfkl icsftmaqmp eakeehsfeh kqlrmelnkl qekhsqqsae lqnaldqiss
     1861 lkekkegmer aisrqlnktk mvlkqtkgnl nvlrardakp ekk
//
LOCUS       XP_077505208            1902 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114981 isoform X20 [Amblyomma
            americanum].
ACCESSION   XP_077505208
VERSION     XP_077505208.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649082.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1902
                     /product="uncharacterized protein LOC144114981 isoform
                     X20"
                     /calculated_mol_wt=213441
     Region          <71..>357
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <759..>1375
                     /region_name="CCDC158"
                     /note="Coiled-coil domain-containing protein 158;
                     pfam15921"
                     /db_xref="CDD:464943"
     Region          <1097..>1386
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          <1271..1569
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1902
                     /gene="LOC144114981"
                     /coded_by="XM_077649082.1:162..5870"
                     /db_xref="GeneID:144114981"
ORIGIN      
        1 mdnpqsness aaesrpetnl dlpassmeap tsgshgavan qehslrnyeq niislrqenl
       61 rlrlrsfylq lqtktpaeen enefknvaeq iqkfvtelde rdklldesrt kreeleklva
      121 nlihekaale delckwkmkt nvseaaqddn vkmvstlshv kkeledkvkq leqqldelkd
      181 kkvpslkrql dtrtaalqai sahaakqeea lkslhnvhsl csliqpktea flqliddsla
      241 agdiqatqra kndfqrelsk issehcsfql dsteqpcsva afsdrvwkle aelqsrenli
      301 eclnsdiacl tsrleatqtr cleyetemkr aseihqkica ekdklldsvq rkmqemerha
      361 qeglspksrt selqlecnrl hiavleksrf inellsernk atveteksfh elllsmkqkd
      421 qliemmqtkf ehaeegeavi eneglrqpnp adflletceg qpkpaepgtp lgterpsfqg
      481 sspaspegpd eptvfylsqs vprevkvlyq ilsdlaqesg impelstval tnisciqkvt
      541 eilksahekg lrfslpiqst ivnmdsldhc epderglaaw eptvlvklge klqqlaylfa
      601 vdgpvvskwq madvvqlldt vlekakqeaa nklflhrspe dlppvlfgkg gqpkeheelt
      661 hsamlsphlr nerghsdisd yqeellqdlg salkcvgdlh nevkalpltp tsnetrkstg
      721 stdelifavd ssgqlvpvpv dqaghfekda ssqteepcdn leslkselnn akaqvvskhk
      781 deslseqagv avvqaleekn aalqeqlhqm qevstvllcr leelaafleq llaydetgsl
      841 gsvtlsadmv akirqlvads kdfslsfsqs flgsdnnsal sdhllkrgsf drsmqsilsr
      901 skgkndsisv idkpqsckld intippcped iafmedelkt lrdrvqeqqs eissltrrle
      961 eegseraspa aggrskravs ashtkaqkle kllrriqhcn srledtltvh kelcqkllri
     1021 psgdgpkdas esqqpkdlte slvpsmrklq frleemvinn evlqeilteh vasgkavlte
     1081 pctpggagan skegaaeeln qckkelacke rredilkram vheqdakvql ekeladcqke
     1141 lqslheahca lekqyktavq rnsdlalrdp qrrssfeksr lerargssfe rpdgeemklr
     1201 denhtltael rqqaqeiarl gkeealhkde vsnlsqektv lsqklqavqh ehevllesss
     1261 nkiselvtkl galstqnknq heeirnlrem gesisrenik lkhdldatss eleglrvenq
     1321 dlretllalr kqesllrknl ekavdeqqaa kkisdeysqs nkllrmekdq lgakisdlss
     1381 rldalecekq slyeekqrli levsagedev ervnsklqsc eeecnnlrtq kseveaelqa
     1441 cnnklqefeh laeqlreels atkgklesmd trcnhleqel gaskeahkql lwkrdelqrt
     1501 leavgreaqq skslarmsld kkhvmafmel qdksrslqnv vmelklkler amyenkclrl
     1561 elrvneqfqs gnssgavvgt pdslrsfata asnaspprls phqvpvdssq rtppvvgqhs
     1621 cshsrsyhts pdlgidsdpt pehdvystav eptyvehwqh rmgpdwsiss vhsapdafgg
     1681 scllcqqgkd spaswkfkan atyggeqldh pstqspsksg fpviscslss pgvhlcaivq
     1741 lsdhellkkr vdesiclirr veasvqqald aladfasdct eppecnrllq daergcqsik
     1801 mclmdsfkli csftmaqmpe akeehsfehk qlrmelnklq ekhsqqsael qnaldqissl
     1861 kekkegmera isrqlnktkm vlkqtkgnln vlrardakpe kk
//
LOCUS       XP_077505209            1876 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114981 isoform X21 [Amblyomma
            americanum].
ACCESSION   XP_077505209
VERSION     XP_077505209.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649083.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1876
                     /product="uncharacterized protein LOC144114981 isoform
                     X21"
                     /calculated_mol_wt=210711
     Region          <45..>331
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <733..>1349
                     /region_name="CCDC158"
                     /note="Coiled-coil domain-containing protein 158;
                     pfam15921"
                     /db_xref="CDD:464943"
     Region          <1071..>1360
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          <1245..1543
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1876
                     /gene="LOC144114981"
                     /coded_by="XM_077649083.1:219..5849"
                     /db_xref="GeneID:144114981"
ORIGIN      
        1 meaptsgshg avanqehslr nyeqniislr qenlrlrlrs fylqlqtktp aeenenefkn
       61 vaeqiqkfvt elderdklld esrtkreele klvanlihek aaledelckw kmktnvseaa
      121 qddnvkmvst lshvkkeled kvkqleqqld elkdkkvpsl krqldtrtaa lqaisahaak
      181 qeealkslhn vhslcsliqp kteaflqlid dslaagdiqa tqrakndfqr elskissehc
      241 sfqldsteqp csvaafsdrv wkleaelqsr enlieclnsd iacltsrlea tqtrcleyet
      301 emkraseihq kicaekdkll dsvqrkmqem erhaqeglsp ksrtselqle cnrlhiavle
      361 ksrfinells ernkatvete ksfhelllsm kqkdqliemm qtkfehaeeg eavieneglr
      421 qpnpadflle tcegqpkpae pgtplgterp sfqgsspasp egpdeptvfy lsqsvprevk
      481 vlyqilsdla qesgimpels tvaltnisci qkvteilksa hekglrfslp iqstivnmds
      541 ldhcepderg laaweptvlv klgeklqqla ylfavdgpvv skwqmadvvq lldtvlekak
      601 qeaanklflh rspedlppvl fgkggqpkeh eelthsamls phlrnerghs disdyqeell
      661 qdlgsalkcv gdlhnevkal pltptsnetr kstgstdeli favdssgqlv pvpvdqaghf
      721 ekdassqtee pcdnleslks elnnakaqvv skhkdeslse qagvavvqal eeknaalqeq
      781 lhqmqevstv llcrleelaa fleqllayde tgslgsvtls admvakirql vadskdfsls
      841 fsqsflgsdn nsalsdhllk rgsfdrsmqs ilsrskgknd sisvidkpqs ckldintipp
      901 cpediafmed elktlrdrvq eqqseisslt rrleeegser aspaaggrsk ravsashtka
      961 qklekllrri qhcnsrledt ltvhkelcqk llripsgdgp kdasesqqpk dlteslvpsm
     1021 rklqfrleem vinnevlqei ltehvasgka vltepctpgg aganskegaa eelnqckkel
     1081 ackerredil kramvheqda kvqlekelad cqkelqslhe ahcalekqyk tavqrnsdla
     1141 lrdpqrrssf eksrlerarg ssferpdgee mklrdenhtl taelrqqaqe iarlgkeeal
     1201 hkdevsnlsq ektvlsqklq avqhehevll esssnkisel vtklgalstq nknqheeirn
     1261 lremgesisr eniklkhdld atsseleglr venqdlretl lalrkqesll rknlekavde
     1321 qqaakkisde ysqsnkllrm ekdqlgakis dlssrldale cekqslyeek qrlilevsag
     1381 edevervnsk lqsceeecnn lrtqksevea elqacnnklq efehlaeqlr eelsatkgkl
     1441 esmdtrcnhl eqelgaskea hkqllwkrde lqrtleavgr eaqqskslar msldkkhvma
     1501 fmelqdksrs lqnvvmelkl kleramyenk clrlelrvne qfqsgnssga vvgtpdslrs
     1561 fataasnasp prlsphqvpv dssqrtppvv gqhscshsrs yhtspdlgid sdptpehdvy
     1621 staveptyve hwqhrmgpdw sissvhsapd afggscllcq qgkdspaswk fkanatygge
     1681 qldhpstqsp sksgfpvisc slsspgvhlc aivqlsdhel lkkrvdesic lirrveasvq
     1741 qaldaladfa sdcteppecn rllqdaergc qsikmclmds fklicsftma qmpeakeehs
     1801 fehkqlrmel nklqekhsqq saelqnaldq isslkekkeg meraisrqln ktkmvlkqtk
     1861 gnlnvlrard akpekk
//
LOCUS       XP_077505210             353 aa            linear   INV 25-NOV-2025
DEFINITION  SH3 domain containing GRB2 like, endophilin-B [Amblyomma
            americanum].
ACCESSION   XP_077505210
VERSION     XP_077505210.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649084.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..353
                     /product="SH3 domain containing GRB2 like, endophilin-B"
                     /calculated_mol_wt=39452
     Region          24..249
                     /region_name="BAR_Endophilin_B"
                     /note="The Bin/Amphiphysin/Rvs (BAR) domain of
                     Endophilin-B; cd07594"
                     /db_xref="CDD:153278"
     Site            order(48,51..52,56,58..59,62..63,65..66,92..93,95..96,
                     99..100,102..103,106,120,123..124,211,214..215,217,221,
                     224..225,227..228,231..232,235..236,238..239,242..243,
                     245..246)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153278"
     Site            order(74,155,159,162,167..168,177,180,193)
                     /site_type="other"
                     /note="putative membrane interaction site"
                     /db_xref="CDD:153278"
     Region          297..348
                     /region_name="SH3_Endophilin_B"
                     /note="Src homology 3 domain of Endophilin-B; cd11802"
                     /db_xref="CDD:212736"
     Site            order(302,304,307,311,331..332,345,347..348)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212736"
     CDS             1..353
                     /gene="EndoB"
                     /coded_by="XM_077649084.1:281..1342"
                     /db_xref="GeneID:144114983"
ORIGIN      
        1 mdfsvksfkg laaeaatvft ravqyteerl gtsdktelda hfetlaqrsd rtkywterlv
       61 gkteavlqpn pnvrvedylf ekldkkrdrl nntellgqdm idagtdfgpg tsygsalikv
      121 gqaqqklgqa erefgrlvht nfvqplrrfl dgdmktilke rrllenkrld ldaaknrlrk
      181 akatdpaaek dvrscqsdfd rqaeitrlll egissthvnh lrclsdfvea qmsyyaqcqq
      241 ymtdlqkela nmsiavgagn sgdgsrsgfq gvfsspttsa gspsndvstf insnqkrarv
      301 lydydahdns elslmadevi iisqtdnldp dwmigerglq kgkvpiayle iln
//
LOCUS       XP_077505211             337 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114984 [Amblyomma americanum].
ACCESSION   XP_077505211
VERSION     XP_077505211.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649085.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..337
                     /product="uncharacterized protein LOC144114984"
                     /calculated_mol_wt=37990
     Region          11..95
                     /region_name="FKBP_N_2"
                     /note="BDBT FKBP like N-terminal; pfam18023"
                     /db_xref="CDD:375495"
     Region          169..>337
                     /region_name="3a0801s09"
                     /note="mitochondrial precursor proteins import receptor
                     (72 kDa mitochondrial outermembrane protein)
                     (mitochondrial import receptor for the ADP/ATP carrier)
                     (translocase of outermembrane tom70); TIGR00990"
                     /db_xref="CDD:273380"
     Region          185..217
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          241..268
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          <243..>308
                     /region_name="NlpI"
                     /note="Lipoprotein NlpI, contains TPR repeats [Cell
                     wall/membrane/envelope biogenesis]; COG4785"
                     /db_xref="CDD:443815"
     Region          273..303
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     CDS             1..337
                     /gene="LOC144114984"
                     /coded_by="XM_077649085.1:133..1146"
                     /db_xref="GeneID:144114984"
ORIGIN      
        1 madksvplee weapdksfrk ivvnggcvgk mplhesscrl kvlsdeegii elggatqikt
       61 lviadvssev elilercvmt mnagevcdvf ftlpltvsap yipkaaylyd gsqpepglfn
      121 iprapadcls rsinkeagdv sfdcgvppvk ldvtpksyrv rirlesfsda phvcdmtvad
      181 kwryayehkd kgsrlfsskn yrwafrhfsw sykyvvsleh devpedvasq ielnikdlkl
      241 kcllnlaacq lqncsydyav encaqaleid pknvkalyrr gtaliqlqey erakcdleqa
      301 knldpknpav drqlellker tcklnkyfav amkklfe
//
LOCUS       XP_077505212            1155 aa            linear   INV 25-NOV-2025
DEFINITION  DNA excision repair protein ERCC-6-like [Amblyomma americanum].
ACCESSION   XP_077505212
VERSION     XP_077505212.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649086.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1155
                     /product="DNA excision repair protein ERCC-6-like"
                     /calculated_mol_wt=129936
     Region          <92..589
                     /region_name="HepA"
                     /note="Superfamily II DNA or RNA helicase, SNF2 family
                     [Transcription, Replication, recombination, and repair];
                     COG0553"
                     /db_xref="CDD:440319"
     CDS             1..1155
                     /gene="LOC144114987"
                     /coded_by="XM_077649086.1:205..3672"
                     /db_xref="GeneID:144114987"
ORIGIN      
        1 matsseadte ngkptlfklh ksegkrllke gnvrgalhaf ekavecqpde rletkmkkir
       61 eflaseqdqe gcdndmveig kgfflyeaie rklyayqkeg llwmwglhlk krggvlgddm
      121 glgktiqvva flsgmfdgdm iksvllvmpv slianwkkef qawapgiavy eyhsaskker
      181 ernlarvqrr ggvlltsygm tqtnyeafct qngsqflwcy lildeghkik nptkttkaiy
      241 eipakhrlvl tgtaiqnnlq elwalfnfth qgglvgslat fkrqyetpin rarekdatag
      301 erllgkemsa qlrdliqpyf lrrtkaevle dkvnskddka slcpkltfts kkndlviwiy
      361 lselqkkiyr efleskevan ilmtkksplv qltvlkkicd hprllskrac vqmgmydgms
      421 reeieefldr eegmsmsigd vsdvvllkes gkmtfvlqll ailrdeghrt llfsqsrkil
      481 diiqriltnr gfvvtrldgt inklcerdri vsqfqtknsa dvfllttqvg gvgltltsad
      541 rviiydpswn patdaqavdr ayrigqqknv vvyrlitcss veekiyrrqi fkdsiikqtt
      601 gkqkdpmryf tkqelrelft lenpnysgtq vqlhemhsqg rntnpaledh iavlqkmnif
      661 gvshhdlmfs eesqadaqne fvpgeiehvk erarmaqrmi tfecdmalde iqnkesytvp
      721 mnmvvkprpa eprarspkrn gqnsvpndhd ggksskwnka lvvldddgda vcldgvgsln
      781 kslanmtvqs sdksvsilvd dsdeelfpta ehldsvvids defsdgvevv dspvgtkike
      841 ssfklgvata ehgtsseakt eeqeneahdw qfkmeqpikq qihnedggvv tdrsfpelce
      901 saarlvpevk sehkcaewkl essfsqlntp psppekgsec yslrsswesa qevtskrvls
      961 neepdsdafg tslytvehek rhekkrlwds pnqtsfvqtk qfllsptaaq kegdsedlta
     1021 pkglgtpqnf saklmattts pvklfhstpk stpskriapl fvgspqqqgp lscqkmpqsp
     1081 fqealaksve ydeanliplk vregsaphsn samssvheis sedeqddsds eddvvvrkhp
     1141 nkkaivvsds dyetp
//
LOCUS       XP_077505213             265 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114988 [Amblyomma americanum].
ACCESSION   XP_077505213
VERSION     XP_077505213.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649087.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..265
                     /product="uncharacterized protein LOC144114988"
                     /calculated_mol_wt=30028
     CDS             1..265
                     /gene="LOC144114988"
                     /coded_by="XM_077649087.1:1..798"
                     /db_xref="GeneID:144114988"
ORIGIN      
        1 mehlrppepl rltadggrnw rlfrqkfeff lqatactdkp rseaaktall lsvagedald
       61 vfnnfvfteg eskedyatvi akfeeycveq qneiherasp tedgrspgel lqgrrlrtpv
      121 pdfnplptvr vkkhrqishn eralprlsnn dvvrvngksw srkakvvgsa gprafhvhte
      181 dntilrrnrr hllatkeqws dhssddedfg dnrassqgva stapvapptp aeqsalqplr
      241 rttrqmrppe rlgydqhfqq tsqrc
//
LOCUS       XP_077505214              98 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114989 [Amblyomma americanum].
ACCESSION   XP_077505214
VERSION     XP_077505214.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649088.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..98
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..98
                     /product="uncharacterized protein LOC144114989"
                     /calculated_mol_wt=9959
     CDS             1..98
                     /gene="LOC144114989"
                     /coded_by="XM_077649088.1:7..303"
                     /db_xref="GeneID:144114989"
ORIGIN      
        1 mlmragrkae faewllqvta chagsppdpn lgprssaava ppssavsppa psdealdpdp
       61 klpapalvaa apqapdkeae aipaeglemd ldlwstei
//
LOCUS       XP_077505215             135 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114990 [Amblyomma americanum].
ACCESSION   XP_077505215
VERSION     XP_077505215.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649089.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..135
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..135
                     /product="uncharacterized protein LOC144114990"
                     /calculated_mol_wt=14218
     CDS             1..135
                     /gene="LOC144114990"
                     /coded_by="XM_077649089.1:306..713"
                     /db_xref="GeneID:144114990"
ORIGIN      
        1 mekqaqskrr rspstssrsp hhhslhrrhq aatppgplra gprhlgplfg qsphhcqatp
       61 vtlgpspacs llcqrpsstp largpeppav qaprlpgrsl vdvpswyera gglrfvvave
      121 aagegekaha pgqav
//
LOCUS       XP_077505216             352 aa            linear   INV 25-NOV-2025
DEFINITION  ileal sodium/bile acid cotransporter-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505216
VERSION     XP_077505216.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649090.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..352
                     /product="ileal sodium/bile acid cotransporter-like
                     isoform X1"
                     /calculated_mol_wt=38351
     Region          34..311
                     /region_name="SBF"
                     /note="Sodium Bile acid symporter family; cl19217"
                     /db_xref="CDD:450274"
     CDS             1..352
                     /gene="LOC144114995"
                     /coded_by="XM_077649090.1:420..1478"
                     /db_xref="GeneID:144114995"
ORIGIN      
        1 mgvvsnfsna tnwtagadts pvkyplikvi hdwlivgilv simfgmgchv klselwahlr
       61 rpfgvavgml cqfvmmplva fglltsmrls glyaigmlim accpggtvsn vfayfvdgdv
      121 plsiamtlcs tvlamgmmpv nmllyghyvd tgdivvpysk mamslvfisl paglgmlfnw
      181 lcprvapyvt klastlggml ivatqimevf ifpdifhdip avlyaatvim paagmlfgyl
      241 iswlfrrpda vrktiaiesg iqnvgtalti vslsfqfqeq kqallfpwly gftmiglsfi
      301 lcgiyatykk fcsrssshni apeaettkys ekmqlstvqv pshgvfnpaf qs
//
LOCUS       XP_077505217             352 aa            linear   INV 25-NOV-2025
DEFINITION  ileal sodium/bile acid cotransporter-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505217
VERSION     XP_077505217.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649091.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..352
                     /product="ileal sodium/bile acid cotransporter-like
                     isoform X1"
                     /calculated_mol_wt=38351
     Region          34..311
                     /region_name="SBF"
                     /note="Sodium Bile acid symporter family; cl19217"
                     /db_xref="CDD:450274"
     CDS             1..352
                     /gene="LOC144114995"
                     /coded_by="XM_077649091.1:106..1164"
                     /db_xref="GeneID:144114995"
ORIGIN      
        1 mgvvsnfsna tnwtagadts pvkyplikvi hdwlivgilv simfgmgchv klselwahlr
       61 rpfgvavgml cqfvmmplva fglltsmrls glyaigmlim accpggtvsn vfayfvdgdv
      121 plsiamtlcs tvlamgmmpv nmllyghyvd tgdivvpysk mamslvfisl paglgmlfnw
      181 lcprvapyvt klastlggml ivatqimevf ifpdifhdip avlyaatvim paagmlfgyl
      241 iswlfrrpda vrktiaiesg iqnvgtalti vslsfqfqeq kqallfpwly gftmiglsfi
      301 lcgiyatykk fcsrssshni apeaettkys ekmqlstvqv pshgvfnpaf qs
//
LOCUS       XP_077505218             328 aa            linear   INV 25-NOV-2025
DEFINITION  ileal sodium/bile acid cotransporter-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505218
VERSION     XP_077505218.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649092.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..328
                     /product="ileal sodium/bile acid cotransporter-like
                     isoform X2"
                     /calculated_mol_wt=35724
     Region          34..275
                     /region_name="SBF"
                     /note="Sodium Bile acid symporter family; cl19217"
                     /db_xref="CDD:450274"
     CDS             1..328
                     /gene="LOC144114995"
                     /coded_by="XM_077649092.1:718..1704"
                     /db_xref="GeneID:144114995"
ORIGIN      
        1 mgvvsnfsna tnwtagadts pvkyplikvi hdwlivgilv simfgmgchv klselwahlr
       61 rpfgvavgml cqfvmmplva fglltsmrls glyaigmlim accpggtvsn vfayfvdgdv
      121 plsiamtlcs tvlamgmmpv nmllyghyvd tgdivvpysk mamslvfisl paglgmlfnw
      181 lcprvapyvt klastlggml ivatqimevf ifpdifhdip avlyaatvim paagmlfgyl
      241 iswlfrrpda vrktiaiesg iqnvgtalti vslsfqfqtv kkcscqlckf qatvcstqhf
      301 raeaafkrfs lvctlsqtmn gttfiywl
//
LOCUS       XP_077505219            1021 aa            linear   INV 25-NOV-2025
DEFINITION  GTPase-activating Rap/Ran-GAP domain-like protein 3 isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077505219
VERSION     XP_077505219.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649093.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1021
                     /product="GTPase-activating Rap/Ran-GAP domain-like
                     protein 3 isoform X1"
                     /calculated_mol_wt=115227
     Region          291..470
                     /region_name="Rap_GAP"
                     /note="Rap/ran-GAP; pfam02145"
                     /db_xref="CDD:460463"
     Region          570..849
                     /region_name="CNH"
                     /note="CNH domain; pfam00780"
                     /db_xref="CDD:459938"
     CDS             1..1021
                     /gene="LOC144114996"
                     /coded_by="XM_077649093.1:194..3259"
                     /db_xref="GeneID:144114996"
ORIGIN      
        1 mlrryqgagg saldlaelqr eeimgaapcs sqdppdsake srlrrvftah vrrqskkprh
       61 ggfslnnmlk eplrlvnsvp lnfdfgssss tdlaarrgvf srrhygsvem lascdgdsen
      121 ivrrfrvetg ersdkeeafw tqgkddiclf gspstpvlen peyqtrwyfk yflgklhqny
      181 vgadaektsf flsvvlsdsn tqglqqyrai lwrktgaqri clshtpnkpl tvksilsnfe
      241 tldriekgpk eiftpdiqkd lllleeqegs vnfkfgvlya eagqttddem fsnevgsref
      301 drfvnllgek vrlkgwdkyr ggldvkgdmt gkysvytiye gheiifhvst llpyskdnkq
      361 qverkrhign divniiflng syedhcnfkp smiksqfthi favvsyiree dayrlslfse
      421 esvplfgpsl pcppvfhnhq efrefvlvkl ingekaafnt pifaqkrert ldmlikdlyn
      481 ehmsesrtnm lnrrafsdvl petprgsrrk eearqvefvr igqalkldti vkgdaptsla
      541 ttslfkrepw epqcfypdfp heivcgdswg nnklvlgtes giflieeglt hrmifdktvl
      601 vkqisvveah gillfrtdrg rdsrvhvfrl tdfegdtgns lvrtrsdlre hklertkgch
      661 lyalsrpggs hlrmaaavgr rllvlqwrhs aawttwcaas dtdtvdgfql vrellvpeap
      721 alltlvdspd nhicvgyrhq fdlvsektgd atrlhtvdaa kahlvaaldi yeddeaelll
      781 cynhtthfqk lndenttefd fqwnavpdni vcvfpyimaf tyesieirli ingnlvhnmv
      841 mpklklitsk sdiffvstap effqvrkeri kedrmerdss vsppssphce stgstgsndg
      901 akplrvyris fscltgnspc errvptpslp spqpllatps fgssylspdf qegsrlrpdv
      961 rpsrsatasp vpqmplllpg crsvspkrpg lhkdhthsas sdsglgksvs sspppsphnh
     1021 a
//
LOCUS       XP_077505221            1019 aa            linear   INV 25-NOV-2025
DEFINITION  GTPase-activating Rap/Ran-GAP domain-like protein 3 isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077505221
VERSION     XP_077505221.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649095.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1019
                     /product="GTPase-activating Rap/Ran-GAP domain-like
                     protein 3 isoform X2"
                     /calculated_mol_wt=114995
     Region          291..470
                     /region_name="Rap_GAP"
                     /note="Rap/ran-GAP; pfam02145"
                     /db_xref="CDD:460463"
     Region          570..849
                     /region_name="CNH"
                     /note="CNH domain; pfam00780"
                     /db_xref="CDD:459938"
     CDS             1..1019
                     /gene="LOC144114996"
                     /coded_by="XM_077649095.1:435..3494"
                     /db_xref="GeneID:144114996"
ORIGIN      
        1 mlrryqgagg saldlaelqr eeimgaapcs sqdppdsake srlrrvftah vrrqskkprh
       61 ggfslnnmlk eplrlvnsvp lnfdfgssss tdlaarrgvf srrhygsvem lascdgdsen
      121 ivrrfrvetg ersdkeeafw tqgkddiclf gspstpvlen peyqtrwyfk yflgklhqny
      181 vgadaektsf flsvvlsdsn tqglqqyrai lwrktgaqri clshtpnkpl tvksilsnfe
      241 tldriekgpk eiftpdiqkd lllleeqegs vnfkfgvlya eagqttddem fsnevgsref
      301 drfvnllgek vrlkgwdkyr ggldvkgdmt gkysvytiye gheiifhvst llpyskdnkq
      361 qverkrhign divniiflng syedhcnfkp smiksqfthi favvsyiree dayrlslfse
      421 esvplfgpsl pcppvfhnhq efrefvlvkl ingekaafnt pifaqkrert ldmlikdlyn
      481 ehmsesrtnm lnrrafsdvl petprgsrrk eearqvefvr igqalkldti vkgdaptsla
      541 ttslfkrepw epqcfypdfp heivcgdswg nnklvlgtes giflieeglt hrmifdktvl
      601 vkqisvveah gillfrtdrg rdsrvhvfrl tdfegdtgns lvrtrsdlre hklertkgch
      661 lyalsrpggs hlrmaaavgr rllvlqwrhs aawttwcaas dtdtvdgfql vrellvpeap
      721 alltlvdspd nhicvgyrhq fdlvsektgd atrlhtvdaa kahlvaaldi yeddeaelll
      781 cynhtthfqk lndenttefd fqwnavpdni vcvfpyimaf tyesieirli ingnlvhnmv
      841 mpklklitsk sdiffvstap effqvrkeri kedrmerdss vsppssphst gstgsndgak
      901 plrvyrisfs cltgnspcer rvptpslpsp qpllatpsfg ssylspdfqe gsrlrpdvrp
      961 srsataspvp qmplllpgcr svspkrpglh kdhthsassd sglgksvsss pppsphnha
//
LOCUS       XP_077505222            1012 aa            linear   INV 25-NOV-2025
DEFINITION  GTPase-activating Rap/Ran-GAP domain-like protein 3 isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077505222
VERSION     XP_077505222.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649096.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1012
                     /product="GTPase-activating Rap/Ran-GAP domain-like
                     protein 3 isoform X3"
                     /calculated_mol_wt=113947
     Region          282..461
                     /region_name="Rap_GAP"
                     /note="Rap/ran-GAP; pfam02145"
                     /db_xref="CDD:460463"
     Region          561..840
                     /region_name="CNH"
                     /note="CNH domain; pfam00780"
                     /db_xref="CDD:459938"
     CDS             1..1012
                     /gene="LOC144114996"
                     /coded_by="XM_077649096.1:1907..4945"
                     /db_xref="GeneID:144114996"
ORIGIN      
        1 mdrrkklplr lssaaavpsv kvtlppkrdr srslcvdrlp pliarsppav aravtlgrrc
       61 sasgtadrlt vhtcyghgss stdlaarrgv fsrrhygsve mlascdgdse nivrrfrvet
      121 gersdkeeaf wtqgkddicl fgspstpvle npeyqtrwyf kyflgklhqn yvgadaekts
      181 fflsvvlsds ntqglqqyra ilwrktgaqr iclshtpnkp ltvksilsnf etldriekgp
      241 keiftpdiqk dlllleeqeg svnfkfgvly aeagqttdde mfsnevgsre fdrfvnllge
      301 kvrlkgwdky rggldvkgdm tgkysvytiy egheiifhvs tllpyskdnk qqverkrhig
      361 ndivniifln gsyedhcnfk psmiksqfth ifavvsyire edayrlslfs eesvplfgps
      421 lpcppvfhnh qefrefvlvk lingekaafn tpifaqkrer tldmlikdly nehmsesrtn
      481 mlnrrafsdv lpetprgsrr keearqvefv rigqalkldt ivkgdaptsl attslfkrep
      541 wepqcfypdf pheivcgdsw gnnklvlgte sgiflieegl thrmifdktv lvkqisvvea
      601 hgillfrtdr grdsrvhvfr ltdfegdtgn slvrtrsdlr ehklertkgc hlyalsrpgg
      661 shlrmaaavg rrllvlqwrh saawttwcaa sdtdtvdgfq lvrellvpea palltlvdsp
      721 dnhicvgyrh qfdlvsektg datrlhtvda akahlvaald iyeddeaell lcynhtthfq
      781 klndenttef dfqwnavpdn ivcvfpyima ftyesieirl iingnlvhnm vmpklklits
      841 ksdiffvsta peffqvrker ikedrmerds svsppssphc estgstgsnd gakplrvyri
      901 sfscltgnsp cerrvptpsl pspqpllatp sfgssylspd fqegsrlrpd vrpsrsatas
      961 pvpqmplllp gcrsvspkrp glhkdhthsa ssdsglgksv ssspppsphn ha
//
LOCUS       XP_077505223            1010 aa            linear   INV 25-NOV-2025
DEFINITION  GTPase-activating Rap/Ran-GAP domain-like protein 3 isoform X4
            [Amblyomma americanum].
ACCESSION   XP_077505223
VERSION     XP_077505223.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649097.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1010
                     /product="GTPase-activating Rap/Ran-GAP domain-like
                     protein 3 isoform X4"
                     /calculated_mol_wt=113715
     Region          282..461
                     /region_name="Rap_GAP"
                     /note="Rap/ran-GAP; pfam02145"
                     /db_xref="CDD:460463"
     Region          561..840
                     /region_name="CNH"
                     /note="CNH domain; pfam00780"
                     /db_xref="CDD:459938"
     CDS             1..1010
                     /gene="LOC144114996"
                     /coded_by="XM_077649097.1:1907..4939"
                     /db_xref="GeneID:144114996"
ORIGIN      
        1 mdrrkklplr lssaaavpsv kvtlppkrdr srslcvdrlp pliarsppav aravtlgrrc
       61 sasgtadrlt vhtcyghgss stdlaarrgv fsrrhygsve mlascdgdse nivrrfrvet
      121 gersdkeeaf wtqgkddicl fgspstpvle npeyqtrwyf kyflgklhqn yvgadaekts
      181 fflsvvlsds ntqglqqyra ilwrktgaqr iclshtpnkp ltvksilsnf etldriekgp
      241 keiftpdiqk dlllleeqeg svnfkfgvly aeagqttdde mfsnevgsre fdrfvnllge
      301 kvrlkgwdky rggldvkgdm tgkysvytiy egheiifhvs tllpyskdnk qqverkrhig
      361 ndivniifln gsyedhcnfk psmiksqfth ifavvsyire edayrlslfs eesvplfgps
      421 lpcppvfhnh qefrefvlvk lingekaafn tpifaqkrer tldmlikdly nehmsesrtn
      481 mlnrrafsdv lpetprgsrr keearqvefv rigqalkldt ivkgdaptsl attslfkrep
      541 wepqcfypdf pheivcgdsw gnnklvlgte sgiflieegl thrmifdktv lvkqisvvea
      601 hgillfrtdr grdsrvhvfr ltdfegdtgn slvrtrsdlr ehklertkgc hlyalsrpgg
      661 shlrmaaavg rrllvlqwrh saawttwcaa sdtdtvdgfq lvrellvpea palltlvdsp
      721 dnhicvgyrh qfdlvsektg datrlhtvda akahlvaald iyeddeaell lcynhtthfq
      781 klndenttef dfqwnavpdn ivcvfpyima ftyesieirl iingnlvhnm vmpklklits
      841 ksdiffvsta peffqvrker ikedrmerds svsppssphs tgstgsndga kplrvyrisf
      901 scltgnspce rrvptpslps pqpllatpsf gssylspdfq egsrlrpdvr psrsataspv
      961 pqmplllpgc rsvspkrpgl hkdhthsass dsglgksvss spppsphnha
//
LOCUS       XP_077505224            1010 aa            linear   INV 25-NOV-2025
DEFINITION  GTPase-activating Rap/Ran-GAP domain-like protein 3 isoform X5
            [Amblyomma americanum].
ACCESSION   XP_077505224
VERSION     XP_077505224.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649098.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1010
                     /product="GTPase-activating Rap/Ran-GAP domain-like
                     protein 3 isoform X5"
                     /calculated_mol_wt=113962
     Region          280..459
                     /region_name="Rap_GAP"
                     /note="Rap/ran-GAP; pfam02145"
                     /db_xref="CDD:460463"
     Region          559..838
                     /region_name="CNH"
                     /note="CNH domain; pfam00780"
                     /db_xref="CDD:459938"
     CDS             1..1010
                     /gene="LOC144114996"
                     /coded_by="XM_077649098.1:192..3224"
                     /db_xref="GeneID:144114996"
ORIGIN      
        1 mlrryqgagg saldlaelqr eeimgaapcs sqdppdsake srlrrvftah vrrqskkprh
       61 ggfslnnmlk eplrlvnsvp lnfdfgssss tdlaarrgvf srrhygsvem lascdgdsen
      121 ivrrfrvetg ersdkeelfg spstpvlenp eyqtrwyfky flgklhqnyv gadaektsff
      181 lsvvlsdsnt qglqqyrail wrktgaqric lshtpnkplt vksilsnfet ldriekgpke
      241 iftpdiqkdl llleeqegsv nfkfgvlyae agqttddemf snevgsrefd rfvnllgekv
      301 rlkgwdkyrg gldvkgdmtg kysvytiyeg heiifhvstl lpyskdnkqq verkrhignd
      361 ivniiflngs yedhcnfkps miksqfthif avvsyireed ayrlslfsee svplfgpslp
      421 cppvfhnhqe frefvlvkli ngekaafntp ifaqkrertl dmlikdlyne hmsesrtnml
      481 nrrafsdvlp etprgsrrke earqvefvri gqalkldtiv kgdaptslat tslfkrepwe
      541 pqcfypdfph eivcgdswgn nklvlgtesg iflieeglth rmifdktvlv kqisvveahg
      601 illfrtdrgr dsrvhvfrlt dfegdtgnsl vrtrsdlreh klertkgchl yalsrpggsh
      661 lrmaaavgrr llvlqwrhsa awttwcaasd tdtvdgfqlv rellvpeapa lltlvdspdn
      721 hicvgyrhqf dlvsektgda trlhtvdaak ahlvaaldiy eddeaelllc ynhtthfqkl
      781 ndenttefdf qwnavpdniv cvfpyimaft yesieirlii ngnlvhnmvm pklklitsks
      841 diffvstape ffqvrkerik edrmerdssv sppssphces tgstgsndga kplrvyrisf
      901 scltgnspce rrvptpslps pqpllatpsf gssylspdfq egsrlrpdvr psrsataspv
      961 pqmplllpgc rsvspkrpgl hkdhthsass dsglgksvss spppsphnha
//
LOCUS       XP_077505225            1009 aa            linear   INV 25-NOV-2025
DEFINITION  GTPase-activating Rap/Ran-GAP domain-like protein 3 isoform X6
            [Amblyomma americanum].
ACCESSION   XP_077505225
VERSION     XP_077505225.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649099.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1009
                     /product="GTPase-activating Rap/Ran-GAP domain-like
                     protein 3 isoform X6"
                     /calculated_mol_wt=113579
     Region          279..458
                     /region_name="Rap_GAP"
                     /note="Rap/ran-GAP; pfam02145"
                     /db_xref="CDD:460463"
     Region          558..837
                     /region_name="CNH"
                     /note="CNH domain; pfam00780"
                     /db_xref="CDD:459938"
     CDS             1..1009
                     /gene="LOC144114996"
                     /coded_by="XM_077649099.1:110..3139"
                     /db_xref="GeneID:144114996"
ORIGIN      
        1 maeakcrspg srageaaapk rhsgrplfli sspskssssg glchpmlikv qlnddqrrsc
       61 svdipssrtv vpwtrssstd laarrgvfsr rhygsvemla scdgdseniv rrfrvetger
      121 sdkeeafwtq gkddiclfgs pstpvlenpe yqtrwyfkyf lgklhqnyvg adaektsffl
      181 svvlsdsntq glqqyrailw rktgaqricl shtpnkpltv ksilsnfetl driekgpkei
      241 ftpdiqkdll lleeqegsvn fkfgvlyaea gqttddemfs nevgsrefdr fvnllgekvr
      301 lkgwdkyrgg ldvkgdmtgk ysvytiyegh eiifhvstll pyskdnkqqv erkrhigndi
      361 vniiflngsy edhcnfkpsm iksqfthifa vvsyireeda yrlslfsees vplfgpslpc
      421 ppvfhnhqef refvlvklin gekaafntpi faqkrertld mlikdlyneh msesrtnmln
      481 rrafsdvlpe tprgsrrkee arqvefvrig qalkldtivk gdaptslatt slfkrepwep
      541 qcfypdfphe ivcgdswgnn klvlgtesgi flieeglthr mifdktvlvk qisvveahgi
      601 llfrtdrgrd srvhvfrltd fegdtgnslv rtrsdlrehk lertkgchly alsrpggshl
      661 rmaaavgrrl lvlqwrhsaa wttwcaasdt dtvdgfqlvr ellvpeapal ltlvdspdnh
      721 icvgyrhqfd lvsektgdat rlhtvdaaka hlvaaldiye ddeaelllcy nhtthfqkln
      781 denttefdfq wnavpdnivc vfpyimafty esieirliin gnlvhnmvmp klklitsksd
      841 iffvstapef fqvrkerike drmerdssvs ppssphcest gstgsndgak plrvyrisfs
      901 cltgnspcer rvptpslpsp qpllatpsfg ssylspdfqe gsrlrpdvrp srsataspvp
      961 qmplllpgcr svspkrpglh kdhthsassd sglgksvsss pppsphnha
//
LOCUS       XP_077505226            1008 aa            linear   INV 25-NOV-2025
DEFINITION  GTPase-activating Rap/Ran-GAP domain-like protein 3 isoform X7
            [Amblyomma americanum].
ACCESSION   XP_077505226
VERSION     XP_077505226.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649100.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1008
                     /product="GTPase-activating Rap/Ran-GAP domain-like
                     protein 3 isoform X7"
                     /calculated_mol_wt=113729
     Region          280..459
                     /region_name="Rap_GAP"
                     /note="Rap/ran-GAP; pfam02145"
                     /db_xref="CDD:460463"
     Region          559..838
                     /region_name="CNH"
                     /note="CNH domain; pfam00780"
                     /db_xref="CDD:459938"
     CDS             1..1008
                     /gene="LOC144114996"
                     /coded_by="XM_077649100.1:191..3217"
                     /db_xref="GeneID:144114996"
ORIGIN      
        1 mlrryqgagg saldlaelqr eeimgaapcs sqdppdsake srlrrvftah vrrqskkprh
       61 ggfslnnmlk eplrlvnsvp lnfdfgssss tdlaarrgvf srrhygsvem lascdgdsen
      121 ivrrfrvetg ersdkeelfg spstpvlenp eyqtrwyfky flgklhqnyv gadaektsff
      181 lsvvlsdsnt qglqqyrail wrktgaqric lshtpnkplt vksilsnfet ldriekgpke
      241 iftpdiqkdl llleeqegsv nfkfgvlyae agqttddemf snevgsrefd rfvnllgekv
      301 rlkgwdkyrg gldvkgdmtg kysvytiyeg heiifhvstl lpyskdnkqq verkrhignd
      361 ivniiflngs yedhcnfkps miksqfthif avvsyireed ayrlslfsee svplfgpslp
      421 cppvfhnhqe frefvlvkli ngekaafntp ifaqkrertl dmlikdlyne hmsesrtnml
      481 nrrafsdvlp etprgsrrke earqvefvri gqalkldtiv kgdaptslat tslfkrepwe
      541 pqcfypdfph eivcgdswgn nklvlgtesg iflieeglth rmifdktvlv kqisvveahg
      601 illfrtdrgr dsrvhvfrlt dfegdtgnsl vrtrsdlreh klertkgchl yalsrpggsh
      661 lrmaaavgrr llvlqwrhsa awttwcaasd tdtvdgfqlv rellvpeapa lltlvdspdn
      721 hicvgyrhqf dlvsektgda trlhtvdaak ahlvaaldiy eddeaelllc ynhtthfqkl
      781 ndenttefdf qwnavpdniv cvfpyimaft yesieirlii ngnlvhnmvm pklklitsks
      841 diffvstape ffqvrkerik edrmerdssv sppssphstg stgsndgakp lrvyrisfsc
      901 ltgnspcerr vptpslpspq pllatpsfgs sylspdfqeg srlrpdvrps rsataspvpq
      961 mplllpgcrs vspkrpglhk dhthsassds glgksvsssp ppsphnha
//
LOCUS       XP_077505227            1007 aa            linear   INV 25-NOV-2025
DEFINITION  GTPase-activating Rap/Ran-GAP domain-like protein 3 isoform X8
            [Amblyomma americanum].
ACCESSION   XP_077505227
VERSION     XP_077505227.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649101.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1007
                     /product="GTPase-activating Rap/Ran-GAP domain-like
                     protein 3 isoform X8"
                     /calculated_mol_wt=113347
     Region          279..458
                     /region_name="Rap_GAP"
                     /note="Rap/ran-GAP; pfam02145"
                     /db_xref="CDD:460463"
     Region          558..837
                     /region_name="CNH"
                     /note="CNH domain; pfam00780"
                     /db_xref="CDD:459938"
     CDS             1..1007
                     /gene="LOC144114996"
                     /coded_by="XM_077649101.1:110..3133"
                     /db_xref="GeneID:144114996"
ORIGIN      
        1 maeakcrspg srageaaapk rhsgrplfli sspskssssg glchpmlikv qlnddqrrsc
       61 svdipssrtv vpwtrssstd laarrgvfsr rhygsvemla scdgdseniv rrfrvetger
      121 sdkeeafwtq gkddiclfgs pstpvlenpe yqtrwyfkyf lgklhqnyvg adaektsffl
      181 svvlsdsntq glqqyrailw rktgaqricl shtpnkpltv ksilsnfetl driekgpkei
      241 ftpdiqkdll lleeqegsvn fkfgvlyaea gqttddemfs nevgsrefdr fvnllgekvr
      301 lkgwdkyrgg ldvkgdmtgk ysvytiyegh eiifhvstll pyskdnkqqv erkrhigndi
      361 vniiflngsy edhcnfkpsm iksqfthifa vvsyireeda yrlslfsees vplfgpslpc
      421 ppvfhnhqef refvlvklin gekaafntpi faqkrertld mlikdlyneh msesrtnmln
      481 rrafsdvlpe tprgsrrkee arqvefvrig qalkldtivk gdaptslatt slfkrepwep
      541 qcfypdfphe ivcgdswgnn klvlgtesgi flieeglthr mifdktvlvk qisvveahgi
      601 llfrtdrgrd srvhvfrltd fegdtgnslv rtrsdlrehk lertkgchly alsrpggshl
      661 rmaaavgrrl lvlqwrhsaa wttwcaasdt dtvdgfqlvr ellvpeapal ltlvdspdnh
      721 icvgyrhqfd lvsektgdat rlhtvdaaka hlvaaldiye ddeaelllcy nhtthfqkln
      781 denttefdfq wnavpdnivc vfpyimafty esieirliin gnlvhnmvmp klklitsksd
      841 iffvstapef fqvrkerike drmerdssvs ppssphstgs tgsndgakpl rvyrisfscl
      901 tgnspcerrv ptpslpspqp llatpsfgss ylspdfqegs rlrpdvrpsr sataspvpqm
      961 plllpgcrsv spkrpglhkd hthsassdsg lgksvssspp psphnha
//
LOCUS       XP_077505228             999 aa            linear   INV 25-NOV-2025
DEFINITION  GTPase-activating Rap/Ran-GAP domain-like protein 3 isoform X9
            [Amblyomma americanum].
ACCESSION   XP_077505228
VERSION     XP_077505228.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649102.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..999
                     /product="GTPase-activating Rap/Ran-GAP domain-like
                     protein 3 isoform X9"
                     /calculated_mol_wt=113102
     Region          269..448
                     /region_name="Rap_GAP"
                     /note="Rap/ran-GAP; pfam02145"
                     /db_xref="CDD:460463"
     Region          548..827
                     /region_name="CNH"
                     /note="CNH domain; pfam00780"
                     /db_xref="CDD:459938"
     CDS             1..999
                     /gene="LOC144114996"
                     /coded_by="XM_077649102.1:371..3370"
                     /db_xref="GeneID:144114996"
ORIGIN      
        1 mlmnirqvrr rrftrsenty eggasssvpf swnsliresl lqtswpisls adsdddefdl
       61 fnprsssstd laarrgvfsr rhygsvemla scdgdseniv rrfrvetger sdkeeafwtq
      121 gkddiclfgs pstpvlenpe yqtrwyfkyf lgklhqnyvg adaektsffl svvlsdsntq
      181 glqqyrailw rktgaqricl shtpnkpltv ksilsnfetl driekgpkei ftpdiqkdll
      241 lleeqegsvn fkfgvlyaea gqttddemfs nevgsrefdr fvnllgekvr lkgwdkyrgg
      301 ldvkgdmtgk ysvytiyegh eiifhvstll pyskdnkqqv erkrhigndi vniiflngsy
      361 edhcnfkpsm iksqfthifa vvsyireeda yrlslfsees vplfgpslpc ppvfhnhqef
      421 refvlvklin gekaafntpi faqkrertld mlikdlyneh msesrtnmln rrafsdvlpe
      481 tprgsrrkee arqvefvrig qalkldtivk gdaptslatt slfkrepwep qcfypdfphe
      541 ivcgdswgnn klvlgtesgi flieeglthr mifdktvlvk qisvveahgi llfrtdrgrd
      601 srvhvfrltd fegdtgnslv rtrsdlrehk lertkgchly alsrpggshl rmaaavgrrl
      661 lvlqwrhsaa wttwcaasdt dtvdgfqlvr ellvpeapal ltlvdspdnh icvgyrhqfd
      721 lvsektgdat rlhtvdaaka hlvaaldiye ddeaelllcy nhtthfqkln denttefdfq
      781 wnavpdnivc vfpyimafty esieirliin gnlvhnmvmp klklitsksd iffvstapef
      841 fqvrkerike drmerdssvs ppssphcest gstgsndgak plrvyrisfs cltgnspcer
      901 rvptpslpsp qpllatpsfg ssylspdfqe gsrlrpdvrp srsataspvp qmplllpgcr
      961 svspkrpglh kdhthsassd sglgksvsss pppsphnha
//
LOCUS       XP_077505229             999 aa            linear   INV 25-NOV-2025
DEFINITION  GTPase-activating Rap/Ran-GAP domain-like protein 3 isoform X10
            [Amblyomma americanum].
ACCESSION   XP_077505229
VERSION     XP_077505229.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649103.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..999
                     /product="GTPase-activating Rap/Ran-GAP domain-like
                     protein 3 isoform X10"
                     /calculated_mol_wt=112449
     Region          47..141
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          271..450
                     /region_name="Rap_GAP"
                     /note="Rap/ran-GAP; pfam02145"
                     /db_xref="CDD:460463"
     Region          550..829
                     /region_name="CNH"
                     /note="CNH domain; pfam00780"
                     /db_xref="CDD:459938"
     CDS             1..999
                     /gene="LOC144114996"
                     /coded_by="XM_077649103.1:1926..4925"
                     /db_xref="GeneID:144114996"
ORIGIN      
        1 mdrrkklplr lssaaavpsv kvtlppkrdr srslcvdrlp pliarsppav aravtlgrrc
       61 sasgtadrlt vhtcyghgss stdlaarrgv fsrrhygsve mlascdgdse nivrrfrvet
      121 gersdkeelf gspstpvlen peyqtrwyfk yflgklhqny vgadaektsf flsvvlsdsn
      181 tqglqqyrai lwrktgaqri clshtpnkpl tvksilsnfe tldriekgpk eiftpdiqkd
      241 lllleeqegs vnfkfgvlya eagqttddem fsnevgsref drfvnllgek vrlkgwdkyr
      301 ggldvkgdmt gkysvytiye gheiifhvst llpyskdnkq qverkrhign divniiflng
      361 syedhcnfkp smiksqfthi favvsyiree dayrlslfse esvplfgpsl pcppvfhnhq
      421 efrefvlvkl ingekaafnt pifaqkrert ldmlikdlyn ehmsesrtnm lnrrafsdvl
      481 petprgsrrk eearqvefvr igqalkldti vkgdaptsla ttslfkrepw epqcfypdfp
      541 heivcgdswg nnklvlgtes giflieeglt hrmifdktvl vkqisvveah gillfrtdrg
      601 rdsrvhvfrl tdfegdtgns lvrtrsdlre hklertkgch lyalsrpggs hlrmaaavgr
      661 rllvlqwrhs aawttwcaas dtdtvdgfql vrellvpeap alltlvdspd nhicvgyrhq
      721 fdlvsektgd atrlhtvdaa kahlvaaldi yeddeaelll cynhtthfqk lndenttefd
      781 fqwnavpdni vcvfpyimaf tyesieirli ingnlvhnmv mpklklitsk sdiffvstap
      841 effqvrkeri kedrmerdss vsppssphst gstgsndgak plrvyrisfs cltgnspcer
      901 rvptpslpsp qpllatpsfg ssylspdfqe gsrlrpdvrp srsataspvp qmplllpgcr
      961 svspkrpglh kdhthsassd sglgksvsss pppsphnha
//
LOCUS       XP_077505230             997 aa            linear   INV 25-NOV-2025
DEFINITION  GTPase-activating Rap/Ran-GAP domain-like protein 3 isoform X11
            [Amblyomma americanum].
ACCESSION   XP_077505230
VERSION     XP_077505230.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649104.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..997
                     /product="GTPase-activating Rap/Ran-GAP domain-like
                     protein 3 isoform X11"
                     /calculated_mol_wt=112870
     Region          269..448
                     /region_name="Rap_GAP"
                     /note="Rap/ran-GAP; pfam02145"
                     /db_xref="CDD:460463"
     Region          548..827
                     /region_name="CNH"
                     /note="CNH domain; pfam00780"
                     /db_xref="CDD:459938"
     CDS             1..997
                     /gene="LOC144114996"
                     /coded_by="XM_077649104.1:371..3364"
                     /db_xref="GeneID:144114996"
ORIGIN      
        1 mlmnirqvrr rrftrsenty eggasssvpf swnsliresl lqtswpisls adsdddefdl
       61 fnprsssstd laarrgvfsr rhygsvemla scdgdseniv rrfrvetger sdkeeafwtq
      121 gkddiclfgs pstpvlenpe yqtrwyfkyf lgklhqnyvg adaektsffl svvlsdsntq
      181 glqqyrailw rktgaqricl shtpnkpltv ksilsnfetl driekgpkei ftpdiqkdll
      241 lleeqegsvn fkfgvlyaea gqttddemfs nevgsrefdr fvnllgekvr lkgwdkyrgg
      301 ldvkgdmtgk ysvytiyegh eiifhvstll pyskdnkqqv erkrhigndi vniiflngsy
      361 edhcnfkpsm iksqfthifa vvsyireeda yrlslfsees vplfgpslpc ppvfhnhqef
      421 refvlvklin gekaafntpi faqkrertld mlikdlyneh msesrtnmln rrafsdvlpe
      481 tprgsrrkee arqvefvrig qalkldtivk gdaptslatt slfkrepwep qcfypdfphe
      541 ivcgdswgnn klvlgtesgi flieeglthr mifdktvlvk qisvveahgi llfrtdrgrd
      601 srvhvfrltd fegdtgnslv rtrsdlrehk lertkgchly alsrpggshl rmaaavgrrl
      661 lvlqwrhsaa wttwcaasdt dtvdgfqlvr ellvpeapal ltlvdspdnh icvgyrhqfd
      721 lvsektgdat rlhtvdaaka hlvaaldiye ddeaelllcy nhtthfqkln denttefdfq
      781 wnavpdnivc vfpyimafty esieirliin gnlvhnmvmp klklitsksd iffvstapef
      841 fqvrkerike drmerdssvs ppssphstgs tgsndgakpl rvyrisfscl tgnspcerrv
      901 ptpslpspqp llatpsfgss ylspdfqegs rlrpdvrpsr sataspvpqm plllpgcrsv
      961 spkrpglhkd hthsassdsg lgksvssspp psphnha
//
LOCUS       XP_077505231             988 aa            linear   INV 25-NOV-2025
DEFINITION  GTPase-activating Rap/Ran-GAP domain-like protein 3 isoform X12
            [Amblyomma americanum].
ACCESSION   XP_077505231
VERSION     XP_077505231.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649105.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..988
                     /product="GTPase-activating Rap/Ran-GAP domain-like
                     protein 3 isoform X12"
                     /calculated_mol_wt=111837
     Region          258..437
                     /region_name="Rap_GAP"
                     /note="Rap/ran-GAP; pfam02145"
                     /db_xref="CDD:460463"
     Region          537..816
                     /region_name="CNH"
                     /note="CNH domain; pfam00780"
                     /db_xref="CDD:459938"
     CDS             1..988
                     /gene="LOC144114996"
                     /coded_by="XM_077649105.1:371..3337"
                     /db_xref="GeneID:144114996"
ORIGIN      
        1 mlmnirqvrr rrftrsenty eggasssvpf swnsliresl lqtswpisls adsdddefdl
       61 fnprsssstd laarrgvfsr rhygsvemla scdgdseniv rrfrvetger sdkeelfgsp
      121 stpvlenpey qtrwyfkyfl gklhqnyvga daektsffls vvlsdsntqg lqqyrailwr
      181 ktgaqricls htpnkpltvk silsnfetld riekgpkeif tpdiqkdlll leeqegsvnf
      241 kfgvlyaeag qttddemfsn evgsrefdrf vnllgekvrl kgwdkyrggl dvkgdmtgky
      301 svytiyeghe iifhvstllp yskdnkqqve rkrhigndiv niiflngsye dhcnfkpsmi
      361 ksqfthifav vsyireeday rlslfseesv plfgpslpcp pvfhnhqefr efvlvkling
      421 ekaafntpif aqkrertldm likdlynehm sesrtnmlnr rafsdvlpet prgsrrkeea
      481 rqvefvrigq alkldtivkg daptslatts lfkrepwepq cfypdfphei vcgdswgnnk
      541 lvlgtesgif lieeglthrm ifdktvlvkq isvveahgil lfrtdrgrds rvhvfrltdf
      601 egdtgnslvr trsdlrehkl ertkgchlya lsrpggshlr maaavgrrll vlqwrhsaaw
      661 ttwcaasdtd tvdgfqlvre llvpeapall tlvdspdnhi cvgyrhqfdl vsektgdatr
      721 lhtvdaakah lvaaldiyed deaelllcyn htthfqklnd enttefdfqw navpdnivcv
      781 fpyimaftye sieirliing nlvhnmvmpk lklitsksdi ffvstapeff qvrkeriked
      841 rmerdssvsp pssphcestg stgsndgakp lrvyrisfsc ltgnspcerr vptpslpspq
      901 pllatpsfgs sylspdfqeg srlrpdvrps rsataspvpq mplllpgcrs vspkrpglhk
      961 dhthsassds glgksvsssp ppsphnha
//
LOCUS       XP_077505232             986 aa            linear   INV 25-NOV-2025
DEFINITION  GTPase-activating Rap/Ran-GAP domain-like protein 3 isoform X13
            [Amblyomma americanum].
ACCESSION   XP_077505232
VERSION     XP_077505232.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649106.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..986
                     /product="GTPase-activating Rap/Ran-GAP domain-like
                     protein 3 isoform X13"
                     /calculated_mol_wt=111605
     Region          258..437
                     /region_name="Rap_GAP"
                     /note="Rap/ran-GAP; pfam02145"
                     /db_xref="CDD:460463"
     Region          537..816
                     /region_name="CNH"
                     /note="CNH domain; pfam00780"
                     /db_xref="CDD:459938"
     CDS             1..986
                     /gene="LOC144114996"
                     /coded_by="XM_077649106.1:371..3331"
                     /db_xref="GeneID:144114996"
ORIGIN      
        1 mlmnirqvrr rrftrsenty eggasssvpf swnsliresl lqtswpisls adsdddefdl
       61 fnprsssstd laarrgvfsr rhygsvemla scdgdseniv rrfrvetger sdkeelfgsp
      121 stpvlenpey qtrwyfkyfl gklhqnyvga daektsffls vvlsdsntqg lqqyrailwr
      181 ktgaqricls htpnkpltvk silsnfetld riekgpkeif tpdiqkdlll leeqegsvnf
      241 kfgvlyaeag qttddemfsn evgsrefdrf vnllgekvrl kgwdkyrggl dvkgdmtgky
      301 svytiyeghe iifhvstllp yskdnkqqve rkrhigndiv niiflngsye dhcnfkpsmi
      361 ksqfthifav vsyireeday rlslfseesv plfgpslpcp pvfhnhqefr efvlvkling
      421 ekaafntpif aqkrertldm likdlynehm sesrtnmlnr rafsdvlpet prgsrrkeea
      481 rqvefvrigq alkldtivkg daptslatts lfkrepwepq cfypdfphei vcgdswgnnk
      541 lvlgtesgif lieeglthrm ifdktvlvkq isvveahgil lfrtdrgrds rvhvfrltdf
      601 egdtgnslvr trsdlrehkl ertkgchlya lsrpggshlr maaavgrrll vlqwrhsaaw
      661 ttwcaasdtd tvdgfqlvre llvpeapall tlvdspdnhi cvgyrhqfdl vsektgdatr
      721 lhtvdaakah lvaaldiyed deaelllcyn htthfqklnd enttefdfqw navpdnivcv
      781 fpyimaftye sieirliing nlvhnmvmpk lklitsksdi ffvstapeff qvrkeriked
      841 rmerdssvsp pssphstgst gsndgakplr vyrisfsclt gnspcerrvp tpslpspqpl
      901 latpsfgssy lspdfqegsr lrpdvrpsrs ataspvpqmp lllpgcrsvs pkrpglhkdh
      961 thsassdsgl gksvsssppp sphnha
//
LOCUS       XP_077505233             946 aa            linear   INV 25-NOV-2025
DEFINITION  GTPase-activating Rap/Ran-GAP domain-like protein 3 isoform X14
            [Amblyomma americanum].
ACCESSION   XP_077505233
VERSION     XP_077505233.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649107.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..946
                     /product="GTPase-activating Rap/Ran-GAP domain-like
                     protein 3 isoform X14"
                     /calculated_mol_wt=106738
     Region          216..395
                     /region_name="Rap_GAP"
                     /note="Rap/ran-GAP; pfam02145"
                     /db_xref="CDD:460463"
     Region          495..774
                     /region_name="CNH"
                     /note="CNH domain; pfam00780"
                     /db_xref="CDD:459938"
     CDS             1..946
                     /gene="LOC144114996"
                     /coded_by="XM_077649107.1:108..2948"
                     /db_xref="GeneID:144114996"
ORIGIN      
        1 makawavags esssstdlaa rrgvfsrrhy gsvemlascd gdsenivrrf rvetgersdk
       61 eeafwtqgkd diclfgspst pvlenpeyqt rwyfkyflgk lhqnyvgada ektsfflsvv
      121 lsdsntqglq qyrailwrkt gaqriclsht pnkpltvksi lsnfetldri ekgpkeiftp
      181 diqkdlllle eqegsvnfkf gvlyaeagqt tddemfsnev gsrefdrfvn llgekvrlkg
      241 wdkyrggldv kgdmtgkysv ytiyegheii fhvstllpys kdnkqqverk rhigndivni
      301 iflngsyedh cnfkpsmiks qfthifavvs yireedayrl slfseesvpl fgpslpcppv
      361 fhnhqefref vlvklingek aafntpifaq krertldmli kdlynehmse srtnmlnrra
      421 fsdvlpetpr gsrrkeearq vefvrigqal kldtivkgda ptslattslf krepwepqcf
      481 ypdfpheivc gdswgnnklv lgtesgifli eeglthrmif dktvlvkqis vveahgillf
      541 rtdrgrdsrv hvfrltdfeg dtgnslvrtr sdlrehkler tkgchlyals rpggshlrma
      601 aavgrrllvl qwrhsaawtt wcaasdtdtv dgfqlvrell vpeapalltl vdspdnhicv
      661 gyrhqfdlvs ektgdatrlh tvdaakahlv aaldiyedde aelllcynht thfqklnden
      721 ttefdfqwna vpdnivcvfp yimaftyesi eirliingnl vhnmvmpklk litsksdiff
      781 vstapeffqv rkerikedrm erdssvspps sphcestgst gsndgakplr vyrisfsclt
      841 gnspcerrvp tpslpspqpl latpsfgssy lspdfqegsr lrpdvrpsrs ataspvpqmp
      901 lllpgcrsvs pkrpglhkdh thsassdsgl gksvsssppp sphnha
//
LOCUS       XP_077505234             140 aa            linear   INV 25-NOV-2025
DEFINITION  vesicle-associated membrane protein 4-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505234
VERSION     XP_077505234.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649108.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..140
                     /product="vesicle-associated membrane protein 4-like
                     isoform X1"
                     /calculated_mol_wt=16316
     Region          50..115
                     /region_name="SNARE"
                     /note="SNARE motif; cl22856"
                     /db_xref="CDD:473982"
     Site            order(74,81,84)
                     /site_type="other"
                     /note="flanking leucine-zipper layers"
                     /db_xref="CDD:277192"
     Site            77
                     /site_type="active"
                     /note="zero layer [active]"
                     /db_xref="CDD:277192"
     CDS             1..140
                     /gene="LOC144114998"
                     /coded_by="XM_077649108.1:186..608"
                     /db_xref="GeneID:144114998"
ORIGIN      
        1 mppkfrryls eedvteagep ekekllqdee dedfflkgpt ssgktltiqd kklrrvqfqv
       61 devssimkdn iekimerger ledleeksds latnadqfws takkmqsrmw wrnmrvkvll
      121 vlvilailli ifvpiivrns
//
LOCUS       XP_077505235             113 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144114998 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505235
VERSION     XP_077505235.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649109.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..113
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..113
                     /product="uncharacterized protein LOC144114998 isoform X2"
                     /calculated_mol_wt=13057
     Region          23..88
                     /region_name="SNARE"
                     /note="SNARE motif; cl22856"
                     /db_xref="CDD:473982"
     Site            order(47,54,57)
                     /site_type="other"
                     /note="flanking leucine-zipper layers"
                     /db_xref="CDD:277222"
     Site            50
                     /site_type="active"
                     /note="zero layer [active]"
                     /db_xref="CDD:277222"
     CDS             1..113
                     /gene="LOC144114998"
                     /coded_by="XM_077649109.1:192..533"
                     /db_xref="GeneID:144114998"
ORIGIN      
        1 mllklenlkk gptssgktlt iqdkklrrvq fqvdevssim kdniekimer gerledleek
       61 sdslatnadq fwstakkmqs rmwwrnmrvk vllvlvilai lliifvpiiv rns
//
LOCUS       XP_077505236            1334 aa            linear   INV 25-NOV-2025
DEFINITION  copper-transporting ATPase 2-like [Amblyomma americanum].
ACCESSION   XP_077505236
VERSION     XP_077505236.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649110.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1334
                     /product="copper-transporting ATPase 2-like"
                     /calculated_mol_wt=143384
     Region          5..72
                     /region_name="CopZ"
                     /note="Copper chaperone CopZ [Inorganic ion transport and
                     metabolism]; COG2608"
                     /db_xref="CDD:442020"
     Region          83..147
                     /region_name="CopZ"
                     /note="Copper chaperone CopZ [Inorganic ion transport and
                     metabolism]; COG2608"
                     /db_xref="CDD:442020"
     Region          158..221
                     /region_name="HMA"
                     /note="Heavy-metal-associated domain (HMA) is a conserved
                     domain of approximately 30 amino acid residues found in a
                     number of proteins that transport or detoxify heavy
                     metals, for example, the CPx-type heavy metal ATPases and
                     copper chaperones. HMA domain...; cd00371"
                     /db_xref="CDD:238219"
     Site            order(164..166,169)
                     /site_type="metal-binding"
                     /note="metal-binding site [ion binding]"
                     /db_xref="CDD:238219"
     Region          236..301
                     /region_name="CopZ"
                     /note="Copper chaperone CopZ [Inorganic ion transport and
                     metabolism]; COG2608"
                     /db_xref="CDD:442020"
     Region          373..435
                     /region_name="HMA"
                     /note="Heavy-metal-associated domain (HMA) is a conserved
                     domain of approximately 30 amino acid residues found in a
                     number of proteins that transport or detoxify heavy
                     metals, for example, the CPx-type heavy metal ATPases and
                     copper chaperones. HMA domain...; cd00371"
                     /db_xref="CDD:238219"
     Site            order(378..380,383)
                     /site_type="metal-binding"
                     /note="metal-binding site [ion binding]"
                     /db_xref="CDD:238219"
     Region          448..511
                     /region_name="HMA"
                     /note="Heavy-metal-associated domain (HMA) is a conserved
                     domain of approximately 30 amino acid residues found in a
                     number of proteins that transport or detoxify heavy
                     metals, for example, the CPx-type heavy metal ATPases and
                     copper chaperones. HMA domain...; cd00371"
                     /db_xref="CDD:238219"
     Site            order(454..456,459)
                     /site_type="metal-binding"
                     /note="metal-binding site [ion binding]"
                     /db_xref="CDD:238219"
     Region          534..1242
                     /region_name="P-type_ATPase_Cu-like"
                     /note="P-type heavy metal-transporting ATPase, similar to
                     human copper-transporting ATPases, ATP7A and ATP7B;
                     cd02094"
                     /db_xref="CDD:319783"
     Site            order(859,861,1213)
                     /site_type="other"
                     /note="putative Cu binding site [ion binding]"
                     /db_xref="CDD:319783"
     Site            order(903..905,973,1030..1032,1062,1102..1104,1124,1127,
                     1130,1149,1152)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319783"
     CDS             1..1334
                     /gene="LOC144114999"
                     /coded_by="XM_077649110.1:162..4166"
                     /db_xref="GeneID:144114999"
ORIGIN      
        1 mastmrtdvi llegmtcpvc veiihkklvn lsgvgnvqvd leeneatvey dptevtlrei
       61 traviesgfn artkntrpsl editlliggm mcsecvgkvt tsvaqikgvc rvdvsldkgr
      121 anvtyettqt dplaiqstvn klgfvvtlpe siqpssvtls vdgvacnlcv lsvervllak
      181 pgvqsvfvsl eeqkvlinfc syevkahdlc ravcsagysa tilseqgppd lisalfsvdg
      241 mtcmscvssl erllsqtdgv egtkvsleeg taavvyipsv ltsqkiveii tdagyectvk
      301 eqarrdsivt edtpllemhf nkaascpvis sldsgsgdki fsstgdrlyl paaskvsses
      361 arkkdvplek cylyvrgmtc ascvsavekn llklngvaqa lvsllserae vkydpekvsp
      421 mqlsqmttdl gydasiieti diqpgeidle irgmtcascv asiesnllkq pgvtkasisl
      481 amqrghfvfd teligprkli qaieelgfea spvavnkldv dhlthvaeir kwrrafvisl
      541 icgiptmivm vyfmaladmd nhcclipgls lenlllfifa spvqfvggrh fylpavralr
      601 hgmanmdvlv mlatnvsyly svvillyfva tgadhspttf fdtvpmliif lclgrwlehl
      661 akrhtsdalt klislqatea tllmvnddge vvseqkidas liqrndtikv lpgeripvdg
      721 kvysgssnvn eahitgealp vikkvgstvm agsinengvl titathvgqd ttlaqivrlv
      781 eeaqsskapi qqladkiagy fvpavvalsi ltlavwlvlg firtdlvyny ygkaqnmses
      841 evviqfafqc altvlsiacp calglatpta vmvgtgvgat ngilikgaep lettckvkcf
      901 afdktgtitk gtpvlvyagv ithakgmtln almailgtae assehpiara ivdyakqllq
      961 tdllgscegf qavaglglsc kvgnvasvlk nvssyhtvqd ayyleieyig esslggevqd
     1021 rwddtyrvlv gnrewmkkns vevppsidgl mtkremlgqt vilcaindvl vgvlavsdtl
     1081 kpeskatvke lkgmgfkvvl ltgdntqtas aiaeqvgide vfaqvlpthk aekiqdlqsr
     1141 gfkvamvgdg indspalvra dvgialsdgt dvaieaadvv lirnnlydvv aavdlsrktv
     1201 rrirmnffla siynlvgipl aagvfmpfgi allpwmgaaa malssvsvvt sslllylyqk
     1261 pvlaktacqs eskwssrkdv nltediadsa kpltapvakn ttiqssslrd sflpevciqq
     1321 eneamgsenm qqll
//
LOCUS       XP_077505237             781 aa            linear   INV 25-NOV-2025
DEFINITION  dnaJ homolog subfamily C member 10-like [Amblyomma americanum].
ACCESSION   XP_077505237
VERSION     XP_077505237.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649111.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..781
                     /product="dnaJ homolog subfamily C member 10-like"
                     /calculated_mol_wt=88964
     Region          20..82
                     /region_name="DnaJ"
                     /note="DnaJ domain; pfam00226"
                     /db_xref="CDD:395170"
     Site            order(48..50,59,62..63,66..67)
                     /site_type="other"
                     /note="HSP70 interaction site [polypeptide binding]"
                     /db_xref="CDD:99751"
     Region          117..214
                     /region_name="Thioredoxin_like"
                     /note="Protein Disulfide Oxidoreductases and Other
                     Proteins with a Thioredoxin fold; cl00388"
                     /db_xref="CDD:469754"
     Region          118..537
                     /region_name="PTZ00102"
                     /note="disulphide isomerase; Provisional"
                     /db_xref="CDD:240266"
     Region          437..536
                     /region_name="Thioredoxin_like"
                     /note="Protein Disulfide Oxidoreductases and Other
                     Proteins with a Thioredoxin fold; cl00388"
                     /db_xref="CDD:469754"
     Region          542..652
                     /region_name="Thioredoxin_like"
                     /note="Protein Disulfide Oxidoreductases and Other
                     Proteins with a Thioredoxin fold; cl00388"
                     /db_xref="CDD:469754"
     Region          658..759
                     /region_name="Thioredoxin_like"
                     /note="Protein Disulfide Oxidoreductases and Other
                     Proteins with a Thioredoxin fold; cl00388"
                     /db_xref="CDD:469754"
     CDS             1..781
                     /gene="LOC144115000"
                     /coded_by="XM_077649111.1:149..2494"
                     /db_xref="GeneID:144115000"
ORIGIN      
        1 mraatlilma vfwpaalced yyqllgierd adsrdirraf kklalkfhpd knqgdpeahd
       61 kfvkinkaye vlkdpdirkr ydtygeegld gdqngwgrqy hswnyyyekf giydddpevv
      121 tlsrndfqss vvesedtwfv nyyspqcshc hhlapawrql arsfegviri gavnceedwq
      181 lcrqegihsf pslifypere kyagsrdldd lndfvlrrlp dldvdisdtg llqpeassll
      241 rsvvvascts anvclakeel kklavaldgl ayvslvdcsr eaatceklki sdgvyffeaa
      301 tsqlkkpkdl egsdaselrr avlkelpsfe vitseffksl llqldggdes epwllhftks
      361 gqpdvsqele lkhirgyiqs vrlgtvdcsk evdicsqfsv tksplflllr ssgeyevyhg
      421 rvnardlasf akesagsrlq tltiatfdrk vsqsrntwlv dffapwcppc mrtlpelrkv
      481 srsfegimfg vvdctsdpsv cqsngissyp slvlfhngst tsmsgyktam evkefveisl
      541 dpkvvtltpe tfkelveskg vddvwaidff apwcghcikl apewnkfakm vsdepninvg
      601 qvdceahrhf cnehgvrsyp hiriyprghf ssrhfstfng wsrdaasfrd wamhflpssv
      661 eeldhhgfyk qvledtspwl vdfyapwcgh cvsfrpvfes vakkfegrvk fgavnceehw
      721 hacdaaqvhr yptlmfyvgr takvqsavgs vihdhreadl vnvlelflkk rprtkridde
      781 l
//
LOCUS       XP_077505238             570 aa            linear   INV 25-NOV-2025
DEFINITION  ATP-dependent RNA helicase DDX55 [Amblyomma americanum].
ACCESSION   XP_077505238
VERSION     XP_077505238.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649112.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..570
                     /product="ATP-dependent RNA helicase DDX55"
                     /calculated_mol_wt=64737
     Region          8..423
                     /region_name="SrmB"
                     /note="Superfamily II DNA and RNA helicase [Replication,
                     recombination and repair]; COG0513"
                     /db_xref="CDD:440279"
     Region          18..222
                     /region_name="DEADc_DDX55"
                     /note="DEAD-box helicase domain of DEAD box protein 55;
                     cd17960"
                     /db_xref="CDD:350718"
     Site            order(26..27,29..31,34,52..59)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350718"
     Region          403..459
                     /region_name="DUF4217"
                     /note="Domain of unknown function (DUF4217); pfam13959"
                     /db_xref="CDD:464056"
     CDS             1..570
                     /gene="LOC144115001"
                     /coded_by="XM_077649112.1:162..1874"
                     /db_xref="GeneID:144115001"
ORIGIN      
        1 msenvdsswt slrihdevrk avqelkfekm tpvqattipl flsnkdvave avtgsgktla
       61 flipllerll krdepltkya vgavvisptr elatqiysvl nhflqfvpqf tgqlltggyn
      121 pindvkafke nganiivatp grmvdmlerk denfsfaacv khlevlvlde adrlldmgfe
      181 ksintilsfl pkqrrtglfs atqtkevedl iraglrnpvs vtvkekptes nlmqrtpall
      241 knfyilcead qklstlvafl kshsdekhmv ffstcacvdy fsavlvqllk nmtvihihgk
      301 mkgkrqkvfn rfmkmdkgvl vctdvmargv dipdvqwvlq ydapkssssf ihrcgrtarm
      361 gnegnavvll mrtedayikf lelnqkvtle amkpppvvnd viskvkkmam kdraiyekgv
      421 rafvsyiqay skhecyllfr ikdldfakva egfallkmpy mpelrgkkie gfqpsnidvk
      481 sipykekare kqrqeklksm pegsktdtks akvkasfirs sklkprgkkr ktreeyenee
      541 wdelasdarl lkklkcnkit keefdeafpi
//
LOCUS       XP_077505239             667 aa            linear   INV 25-NOV-2025
DEFINITION  polycomb protein Pcl [Amblyomma americanum].
ACCESSION   XP_077505239
VERSION     XP_077505239.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649113.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..667
                     /product="polycomb protein Pcl"
                     /calculated_mol_wt=74515
     Region          150..201
                     /region_name="PHD_SF"
                     /note="PHD finger superfamily; cl22851"
                     /db_xref="CDD:473978"
     Site            order(150,165..168,172,195)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:276966"
     Region          247..298
                     /region_name="PHD2_MTF2_PHF19_like"
                     /note="PHD finger 2 found in polycomb repressive complex 2
                     (PRC2)-associated polycomb-like (PCL) family proteins
                     MTF2, PHF19, and similar proteins; cd15503"
                     /db_xref="CDD:276978"
     Site            order(247,259..263,267,291)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:276978"
     Region          <640..661
                     /region_name="Mtf2_C"
                     /note="Polycomb-like MTF2 factor 2; pfam14061"
                     /db_xref="CDD:464077"
     CDS             1..667
                     /gene="Pcl"
                     /coded_by="XM_077649113.1:238..2241"
                     /db_xref="GeneID:144115003"
ORIGIN      
        1 mllyamsrga lqggrelrpr vrhkkpkrll rswynlvgke qavpngvdgv degvkdkhrh
       61 nheieeqaqr dtkhrhtshe lkepscnslg ssgehaapgk mrlngnifhg gspekivngr
      121 chtprsdpga khgvdltsde esecedndlv csvcsmgese ppneivicdt cnkgfhqach
      181 tpkisdqvll pnipwhcrnc vpvgsvrksa knykngtrvq mfklgfpynm kslqwddqhk
      241 vntkncycyc ggpgewnmkm lqcrlcrqwf heaciqglet pllygdsfyy ftcsvcnngp
      301 evvkrmqltw mdithltlyn lvmerrkkyy dlddvilpyl nsiwlalqlp sdifrcsvre
      361 rrtniyeclc sdisrfksgk evkkrtslwg lrnrtappip gesvapreqk svvpalppqg
      421 plpkaasgsr vfrragvknm aglrsqengp krrrrkrlqd grlknnasrf cklpnglrrn
      481 kasmqnsgaf qdpfasdeea ersgtgedst fldafipplq dfhggnhpfc devlfppppq
      541 prprpgrppk kltltvplkp aklklrgcqr rlqpgtadtd wedcssmsst cttstgttss
      601 tgtssstggt gvdsstsgqg plrwkealqs aeaasgskgr fivlakrict dgkvqylvdw
      661 dicgnrs
//
LOCUS       XP_077505240             211 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115004 [Amblyomma americanum].
ACCESSION   XP_077505240
VERSION     XP_077505240.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649114.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..211
                     /product="uncharacterized protein LOC144115004"
                     /calculated_mol_wt=23565
     CDS             1..211
                     /gene="LOC144115004"
                     /coded_by="XM_077649114.1:56..691"
                     /db_xref="GeneID:144115004"
ORIGIN      
        1 makhhkavpn dvfdgnsshe htapskkhwr rplrrpwctp rsqqvqggls kmmsviwigd
       61 hhhfwmkrmr tflchqalrp lpaslsllls hqgcctpfcm csvvsdvqps wmvpsipaqq
      121 pgitmqpqdf rltpnleamv nkshgqlgcq gchtlqhlps iqaalcsprs llqlfspfhl
      181 lvpvlsclal lmerwsgriy laiqsssspv g
//
LOCUS       XP_077505241             101 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin-related modifier 1 [Amblyomma americanum].
ACCESSION   XP_077505241
VERSION     XP_077505241.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649115.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..101
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..101
                     /product="ubiquitin-related modifier 1"
                     /calculated_mol_wt=11114
     Region          6..101
                     /region_name="Urm1"
                     /note="Urm1 (Ubiquitin related modifier); pfam09138"
                     /db_xref="CDD:370315"
     CDS             1..101
                     /gene="Urm1"
                     /coded_by="XM_077649115.1:250..555"
                     /db_xref="GeneID:144115005"
ORIGIN      
        1 mtnvihlrve fgggaellfg nqkkhdvaip tgpqkctlsd limwlkenll rgkeelfvqe
       61 ktvrpgilvl vndtdwellg glayelksnd nvlfistlhg g
//
LOCUS       XP_077505242            1893 aa            linear   INV 25-NOV-2025
DEFINITION  cadherin 87A isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505242
VERSION     XP_077505242.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649116.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1893
                     /product="cadherin 87A isoform X1"
                     /calculated_mol_wt=205766
     Region          30..121
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(34..35,81,83,117,119..120)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          129..231
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          241..346
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(248..249,300,302,343,345..346)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          355..462
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(362..363,422,424,458,460..461)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          470..576
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(477..478,539,541,572,574..575)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          585..681
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(591..592,644,648,677,679..680)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          690..786
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(696..697,750,752,783,785..786)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          795..908
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(802..803,871,873,905,907..908)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          923..1015
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(930..931,978,980,1011,1013..1014)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1024..1122
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1030..1031,1083,1085,1119,1121..1122)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1131..1228
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          1243..1344
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1248..1249,1306,1308,1340,1342..1343)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1356..1472
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1363..1364,1416,1418,1468,1470..1471)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     CDS             1..1893
                     /gene="Cad87A"
                     /coded_by="XM_077649116.1:368..6049"
                     /db_xref="GeneID:144115006"
ORIGIN      
        1 malallllsa avhltsanlp pkftktidla vipentpvgt svfrlegsdp enspvyygle
       61 gtdllavdrs tgdvtvinni drevtdtlkf svtledfvgg ldqnnivkvp vsvivldvnd
      121 niprfqnvpy eakisedapv ghtvltnirv tdldsagnvl qvqcfpnnnv peacstfsvv
      181 vtmsspqeln aslvlrkpld havrhvyevn lvaddgsfna tttavvkvtd vqnrppvflg
      241 smsaivsedv pvgtyvmtvk aidgdrgvpr siryslvsnp enffavnknt gvimtanlld
      301 keklgssngv vtvkvkatev egdrlldepg asttaalsit vkdvndeapt fsrpsysvsv
      361 nenippgspl pnldmfvqdt dtgsnsvfni nlvdssgmfs veptiatgst svsirivkgp
      421 ldyenpnqrk fillvvaeea ftnpklssta tvtvnvldin dnapvfeees ytatvvedas
      481 pgsvvttiva tdrdtsphge sglmysllgn gadkfhvdps sgvvtvapce tpgrgncldf
      541 earpsyflsy qatddrgrgq savvpltvrl tdandnppaf eqhlytalid egdvrfdppl
      601 rvqardpdvt sfvkysivsg nsynlftins qtgditvtsr qgldvsllrt dtitltvqas
      661 dggsgidtai vkitvrdann nspvfqkdry vasvpeaspp gtlveqvsat dadtganahv
      721 tyrilkgafd dftidastgv vslsatsrld ydrrssyime iigidggvpe ktgtttlsvn
      781 ilnsndkmpy fqpttqraqv segapvgyrf yklvakdpda sspeflkygi aepvtaiakd
      841 grnvnesvqa ykaffdvdad tgeirvasrl dreaaavvml ttvvtdvsav plqtglgtlv
      901 itivdlndfp psfpppwspd hpelsisvme eqpvgsvvas fvatdpdsni asysiepenp
      961 yfaidklsgv vtihkrvdye qvqelrfsvv vrdtgipqls avamvtatvt nindndptfs
     1021 qkayqasvqe napqgtfvtk veakdidage fgvvsysllg eksgdfqvnk rgeisvagma
     1081 nldreitpai tlqvvatdmg ndattrrsvs vpvyltlldd ndsppvftkk tyeasfvans
     1141 plesaqsivq vsatdadegi naevrysiva gnengvlavn pktgivypvk klessrkeyr
     1201 igleardgag tgpntdncvv lirlieinld kphfvtpslp natvevlenq tlssqiimtv
     1261 eamdkdhgdn grisyyfkvg drnvaetdef ridevtgeih akavldrevr pryelvlvak
     1321 dhgtpapfet lrfltvvlkd iddnaplfpr trstnpyvfh ikenldpgfp vgrvtaidqd
     1381 vgenamvyyy iidgnwdtqf tidkmqgtiy snasfdresk dlfelvvkat snpdylvytr
     1441 qadglppsir sysesdrtva lvnvhiddvn dnapvfinap yyaglrftav egdtvftvra
     1501 rdpdeetadg aaiayriehv tlflpgstgg irpipasfnv sadgrvcath pmaqysqarf
     1561 tvaleakets pphraarttl kvwiyepeql vrviiaqppe evyrdqrrvv avlsnatggt
     1621 vvidklkyhv ndkgklvksw tdlhihvldk ndrvihtpev ldavdsnikl vrfqnpelqi
     1681 hnvlparvgi skqefdlala vliamlvvif vgvvtmmvcc tclkswyayk akealvrhde
     1741 sppvtttenp lwteqklkiy eeqelsmsva pdnlvttead iaafnfnddt ssavyatlrr
     1801 nsvshlgaes rngystltsh rptassrrqr epdaaeshey helhssdarp tgdstpfstf
     1861 rptgkntpte grlfqepsps hksnepvlva ell
//
LOCUS       XP_077505243            1893 aa            linear   INV 25-NOV-2025
DEFINITION  cadherin 87A isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505243
VERSION     XP_077505243.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649117.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1893
                     /product="cadherin 87A isoform X2"
                     /calculated_mol_wt=205770
     Region          30..121
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(34..35,81,83,117,119..120)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          129..231
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          241..346
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(248..249,300,302,343,345..346)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          355..462
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(362..363,422,424,458,460..461)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          470..576
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(477..478,539,541,572,574..575)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          585..681
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(591..592,644,648,677,679..680)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          690..786
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(696..697,750,752,783,785..786)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          795..908
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(802..803,871,873,905,907..908)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          923..1015
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(930..931,978,980,1011,1013..1014)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1024..1122
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1030..1031,1083,1085,1119,1121..1122)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1131..1228
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          1243..1344
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1248..1249,1306,1308,1340,1342..1343)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1356..1472
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1363..1364,1416,1418,1468,1470..1471)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     CDS             1..1893
                     /gene="Cad87A"
                     /coded_by="XM_077649117.1:368..6049"
                     /db_xref="GeneID:144115006"
ORIGIN      
        1 malallllsa avhltsanlp pkftktidla vipentpvgt svfrlegsdp enspvyygle
       61 gtdllavdrs tgdvtvinni drevtdtlkf svtledfvgg ldqnnivkvp vsvivldvnd
      121 niprfqnvpy eakisedapv ghtvltnirv tdldsagnvl qvqcfpnnnv peacstfsvv
      181 vtmsspqeln aslvlrkpld havrhvyevn lvaddgsfna tttavvkvtd vqnrppvflg
      241 smsaivsedv pvgtyvmtvk aidgdrgvpr siryslvsnp enffavnknt gvimtanlld
      301 keklgssngv vtvkvkatev egdrlldepg asttaalsit vkdvndeapt fsrpsysvsv
      361 nenippgspl pnldmfvqdt dtgsnsvfni nlvdssgmfs veptiatgst svsirivkgp
      421 ldyenpnqrk fillvvaeea ftnpklssta tvtvnvldin dnapvfeees ytatvvedas
      481 pgsvvttiva tdrdtsphge sglmysllgn gadkfhvdps sgvvtvapce tpgrgncldf
      541 earpsyflsy qatddrgrgq savvpltvrl tdandnppaf eqhlytalid egdvrfdppl
      601 rvqardpdvt sfvkysivsg nsynlftins qtgditvtsr qgldvsllrt dtitltvqas
      661 dggsgidtai vkitvrdann nspvfqkdry vasvpeaspp gtlveqvsat dadtganahv
      721 tyrilkgafd dftidastgv vslsatsrld ydrrssyime iigidggvpe ktgtttlsvn
      781 ilnsndkmpy fqpttqraqv segapvgyrf yklvakdpda sspeflkygi aepvtaiakd
      841 grnvnesvqa ykaffdvdad tgeirvasrl dreaaavvml ttvvtdvsav plqtglgtlv
      901 itivdlndfp psfpppwspd hpelsisvme eqpvgsvvas fvatdpdsni asysiepenp
      961 yfaidklsgv vtihkrvdye qvqelrfsvv vrdtgipqls avamvtatvt nindndptfs
     1021 qkayqasvqe napqgtfvtk veakdidage fgvvsysllg eksgdfqvnk rgeisvagma
     1081 nldreitpai tlqvvatdmg ndattrrsvs vpvyltlldd ndsppvftkk tyeasfvans
     1141 plesaqsivq vsatdadegi naevrysiva gnengvlavn pktgivypvk klessrkeyr
     1201 igleardgag tgpntdncvv lirlieinld kphfvtpslp natvevlenq tlssqiimtv
     1261 eamdkdhgdn grisyyfkvg drnvaetdef ridevtgeih akavldrevr pryelvlvak
     1321 dhgtpapfet lrfltvvlkd iddnaplfpr trstnpyvfh ikenldpgfp vgrvtaidqd
     1381 vgenamvyyy iidgnwdtqf tidkmqgtiy snasfdresk dlfelvvkat snpdylvytr
     1441 qadglppsir sysesdrtva lvnvhiddvn dnapvfinap yyaglrftav egdtvftvra
     1501 rdpdeetadg aaiayriehv tlflpgstgg irpipasfnv sadgrvcath pmaqysqarf
     1561 tvaleakets pphraarttl kvwiyepeql vrviiaqppe evyrdqrrvv avlsnatggt
     1621 vvidklkyhv ndkgklvksw tdlhihvldk ndrvihtpev ldavdsnikl vrfqnpelqi
     1681 hnvlatrvgi skqefdlala vliamlvvif vgvvtmmvcc tclkswyayk akealvrhde
     1741 sppvtttenp lwteqklkiy eeqelsmsva pdnlvttead iaafnfnddt ssavyatlrr
     1801 nsvshlgaes rngystltsh rptassrrqr epdaaeshey helhssdarp tgdstpfstf
     1861 rptgkntpte grlfqepsps hksnepvlva ell
//
LOCUS       XP_077505245            2771 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115007 [Amblyomma americanum].
ACCESSION   XP_077505245
VERSION     XP_077505245.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649119.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2771
                     /product="uncharacterized protein LOC144115007"
                     /calculated_mol_wt=318307
     Region          156..603
                     /region_name="M1_APN-Q_like"
                     /note="Peptidase M1 aminopeptidase N catalytic domain
                     family which includes aminopeptidase N (APN),
                     aminopeptidase Q (APQ), tricorn interacting factor F3, and
                     endoplasmic reticulum aminopeptidase 1 (ERAP1); cd09601"
                     /db_xref="CDD:341064"
     Site            order(275,277,414..417,450..451,454,473,477,531,536)
                     /site_type="active"
                     /db_xref="CDD:341064"
     Region          686..1004
                     /region_name="ERAP1_C"
                     /note="ERAP1-like C-terminal domain; pfam11838"
                     /db_xref="CDD:463368"
     Region          1042..1484
                     /region_name="M1_APN-Q_like"
                     /note="Peptidase M1 aminopeptidase N catalytic domain
                     family which includes aminopeptidase N (APN),
                     aminopeptidase Q (APQ), tricorn interacting factor F3, and
                     endoplasmic reticulum aminopeptidase 1 (ERAP1); cd09601"
                     /db_xref="CDD:341064"
     Site            order(1163,1165,1299..1302,1335..1336,1339,1358,1362,1416,
                     1421)
                     /site_type="active"
                     /db_xref="CDD:341064"
     Region          1566..1883
                     /region_name="ERAP1_C"
                     /note="ERAP1-like C-terminal domain; pfam11838"
                     /db_xref="CDD:463368"
     Region          1919..2357
                     /region_name="M1_APN-Q_like"
                     /note="Peptidase M1 aminopeptidase N catalytic domain
                     family which includes aminopeptidase N (APN),
                     aminopeptidase Q (APQ), tricorn interacting factor F3, and
                     endoplasmic reticulum aminopeptidase 1 (ERAP1); cd09601"
                     /db_xref="CDD:341064"
     Site            order(2037,2039,2173..2176,2209..2210,2213,2232,2236,2289,
                     2294)
                     /site_type="active"
                     /db_xref="CDD:341064"
     Region          2433..2748
                     /region_name="ERAP1_C"
                     /note="ERAP1-like C-terminal domain; pfam11838"
                     /db_xref="CDD:463368"
     CDS             1..2771
                     /gene="LOC144115007"
                     /coded_by="XM_077649119.1:397..8712"
                     /db_xref="GeneID:144115007"
ORIGIN      
        1 mcdassrdga eskrlemkpv cmaprrgggt vfkatdavar aggsvevdsa asatmsktfh
       61 rrstaacglv vlmlllcsvg lliaflkghq qqtrdshtlh gmtprrgtkn dnfpnrrttq
      121 ppsttttrrp lqtttleppk pkldfrlpat mvplaydltl ephlsrdsfd gevwitvnat
      181 rpvdrfvvhv frltvdetev ffedgsplst gapfeddlne ffvvpvadka gagptpagly
      241 rlrfkfrgsl vggivgfyks sykidnetry latskfqpty arrafpcfde pnfksrfnvt
      301 iihdnnhmal snmpvlsgyp vmredgkmvt kfeesvpmvt ylvcfivcdf ermqtvasvd
      361 nipfsvyaap nqlnkthyal digsrilsfy ekyfglkypl pkqdmiaipd fvsgamehwg
      421 lityrevnll ydtkkssprn kqrvaaviah elahqwfgnl vtmkwwddlw lnegfasyie
      481 ykgidhvepe wdmmnqflte dlqpvmdlds tttshpvvqs vshpdeitei fdtisynkga
      541 svlrmlenfl gpsvfqrgvs sflrnhqfgn akteelwael seaagytlpa gynvssimdt
      601 wtrqmgypvv tisrspddpt lirvvqerfl rnnqhqrngs daspfgykws iplsyktsaa
      661 knkvkqiwlh neqdefhiyd agqhgwvkfn vnqtgyylvn ydptdwqrlg evlqkhheel
      721 tpadrsnlly dafqlawsnr lsydvlfnmt qylihemhli pwstahgsll althllente
      781 vrrpmqtyir klvdpiyktl gwedgdkhmn nmlrrvildl acrnlhqecl keakrrlsaw
      841 vdggqdvprn irgvvyrygi yqssgdhewq fmwekylkev saqerinlly glahvrsppl
      901 ihrylayamn esyvrrqdff svlsflagnp vgrdivwkfv rykwdrlvhr fslndrnfgn
      961 vvkticdyft kqfdynemqa ffhknpnaga grrareqale nvrnniawik hhepsvarwl
     1021 ttnvipepwe nmrlprhvip asyditlepm pvenitvgss tiritvvkat svllvhakni
     1081 nitrttvirh krdtfeevpl gdepflyven dywviqtrdv lppggyvlkf dfsssmrnyl
     1141 sgiykssywd easksnkymv tsqfqptfar mafpcfdepg fkanftitvi hdaemkaisn
     1201 mpvdkirnld dtrvstkfqe shkmttylva isvcdfnyis gntssgtpvr ifarqemlph
     1261 ahyalsatiq vlelfenqfa irfplpklds iaipdpqaaa menwglimys ecmmlynqnm
     1321 tsvsdhystv evishelahm wfgnlvtmkw wddlwlnegf atysshkgie lveptwdaet
     1381 qfllklisgv lekdavlssh pivmpviqts diaeifdiis yskgsavirm lesfmgsgdf
     1441 irglqnylkn rkrssavtld lwkalesvss khqhittimn twtrqmgypc vsvrrlhsng
     1501 sqyeivqqrf llnpddaanl tndspfrytw eipfsyktse eneglhwlrt kkaevinlpt
     1561 sdgntwvkfn nefkgyylvk ydenelktfs qlllenqtvl sasdraeill etfllaragm
     1621 tpyaaamdlt kylrherhfi plmvasrafq hiamclkdhp eknlfldyvk yiteeafeel
     1681 rwrdrgdhlt krarevvlyl scfsgdrvcl qnaadlleaw fagamippnl relvyvwgma
     1741 asgnvtiwea mysrykkell paerkslikg lasidnetvi erllmlslng tvirradfpv
     1801 lvehlatrdi glslawnfir nnwelmssry ssderqlgai lysvckhftt qeqlqkvelf
     1861 feeypeggtg krsslqvlet vranirwrnf fastvlnwvk eyqympwknl rlpahvvpih
     1921 ydlqiqpfld shtfqgqvdi qvellkpvks vhvharslni tsafmtdest kmmvplssmf
     1981 fhepnefyvm qlgntadvgt yslhyefkgv lsrdlrgfyl ssypvganet rylattqfep
     2041 tdarkafpcf deprfkatfs ikiihdpgyi aisnmpvmgk eitttglqvt qfqrtvkmtt
     2101 ylvalivcdf hnisgnssng vevkvfareg eidkahyale aslkiltyfe eyfgikyplp
     2161 kldliaipdf ssgamenwgl itfrearlly dpetsstfdl iticriiahe iahmwfgnlv
     2221 tmewwddlwl negfasyiqy kgmsyvypew epdalftmil agvleadsvt sshpiiqhvs
     2281 npseisslfd visyakgssv lrmlegflsk edfqngisky lskhkfanti tfdlwdeles
     2341 ssssglsvts imeswtkqmg fplvtverng ksfnvrqsqf lidpdtainr kptplkyiwk
     2401 iplsyhtseg ktgllwlakp qqsfkidvse nawvkfnnnm tgvffvkydk knfllledvl
     2461 krdinaftpg drgellveaf flaraghlsy msalnlsryi vqenhyvpwa mfstatsflh
     2521 hrltgtetgk hfkafvrgll tealkgvkfs dtgtqlerlr ratifkvacr sgeksclhva
     2581 shafrswlng eslepnfksi vfhygmrqig destwqqvfq kylqepshae kikimtglgq
     2641 vqdetillry lnytldetki rgqdigtvyg tvvsnpkghd lawnflkdnw kfiiskfgkh
     2701 srtpgrllrl vcsdfntpek mqeikeflke hpetdatkqs rreclqdvdn nikwiskfep
     2761 kvaeslrgll s
//
LOCUS       XP_077505246             863 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115008 [Amblyomma americanum].
ACCESSION   XP_077505246
VERSION     XP_077505246.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649120.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..863
                     /product="uncharacterized protein LOC144115008"
                     /calculated_mol_wt=97424
     Region          4..79
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          173..404
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..863
                     /gene="LOC144115008"
                     /coded_by="XM_077649120.1:100..2691"
                     /db_xref="GeneID:144115008"
ORIGIN      
        1 mpgccvplcs nhsrngwrmf sfprdpkrrl lwtvkikrdk wqpanssric nahfedncye
       61 nhradgrkll ksnavptifs frpmvhhqrk apkergtpal pttsnstvqe qgpgvfadcd
      121 epggtnlsee vvyqpsdgqt eqnegcdahs sssccaqlrk qledvrknhr qlqevhkkan
      181 ltinslrkkv rklksavehf kkrikflnkd qvralsrksn kgsiwspqti kqglqlkfsc
      241 gksgyrtlrq lgyplptert lsrrlhwlkf spgiltdvve llqakaqgme dvekdcvlfi
      301 demeiargfq ldraedivyg gvtlpqkpde panhalvfmv gglnqrwkqv iayhftgshv
      361 dgrtlkdivl qvvqlcadis lrvrvvtsdm gasnramwre lgfsshrnsk ttcsiphpsl
      421 ndmelffnad aahvlknikg qlitshaftl seatasqcdl psrevkldhv havlefdker
      481 elklapklse ahvsyghftk mkvglavqff reapsairyl ikekvldiea ettawflelv
      541 srwyalmssr hpsvalslhd vakhhralet lnlalntfqg msmgstsqwk psqagllist
      601 tvvlrlqdil lnsegysfll tsrllqdcle nlfsvvrlrk pvpdaydmkc alklvcisqf
      661 lhtpsttsys vddseylvdl lsngkrelae aevkeiddse vlfiealtst ecsilfhiag
      721 flvkgilkai nnceqckatl lgssdaenay ltrlkeylen gdnlhypspe vmkvlkscee
      781 hfkgitewae slltmkspvq avitylrqmv pfnvetcqvh neiiqkllys tyartrlrih
      841 lrqleekkvn ghasktcagv slp
//
LOCUS       XP_077505247             402 aa            linear   INV 25-NOV-2025
DEFINITION  RYamide receptor-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505247
VERSION     XP_077505247.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649121.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..402
                     /product="RYamide receptor-like isoform X1"
                     /calculated_mol_wt=45562
     Region          42..327
                     /region_name="7tmA_PR4-like"
                     /note="neuropeptide Y receptor-like found in insect and
                     related proteins, member of the class A family of
                     seven-transmembrane G protein-coupled receptors; cd15392"
                     /db_xref="CDD:320514"
     Region          43..69
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320514"
     Region          76..101
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320514"
     Site            order(98,101..102,115..120,122..123,126,169,171..175,203,
                     206..208,210..212,214..215,272,275..276,278..279,282,
                     294..295,297..299,302,305..306)
                     /site_type="other"
                     /note="putative peptide ligand binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:320514"
     Region          115..145
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320514"
     Region          156..177
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320514"
     Region          203..232
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320514"
     Region          252..282
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320514"
     Region          295..320
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320514"
     CDS             1..402
                     /gene="LOC144115009"
                     /coded_by="XM_077649121.1:5..1213"
                     /db_xref="GeneID:144115009"
ORIGIN      
        1 mdgveesrya eeeaggtnss wandseagsc evspevpqgi qalmylmyia vsiaaiggng
       61 ivcyiviayq rmrtvtnmfi mnlaigdilm aclcipftfv snlllgywpf ggvmcvlvty
      121 aqcvtvfisa ytliaisvdr ytaivyplrp rmtklrskli ialvwivalv tplptalvtq
      181 lvphvcanst yycqeqwgrp eqtavysmal milqyffpll vliftytria vvvwgkqtpg
      241 eaqdardqrm aaskrkmikm mitvvtvfml swlpfntyil lsdldprinn yehiryvyfl
      301 shwlamshts ynpliycwmn akfrdgfcql fqrsrlcwla rlrhrrplrk esaaevaalr
      361 rcntyttyvs vraapgasfr fsgardaaqs ngkprrheds rv
//
LOCUS       XP_077505248             361 aa            linear   INV 25-NOV-2025
DEFINITION  RYamide receptor-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505248
VERSION     XP_077505248.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649122.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..361
                     /product="RYamide receptor-like isoform X2"
                     /calculated_mol_wt=40210
     Region          42..327
                     /region_name="7tmA_PR4-like"
                     /note="neuropeptide Y receptor-like found in insect and
                     related proteins, member of the class A family of
                     seven-transmembrane G protein-coupled receptors; cd15392"
                     /db_xref="CDD:320514"
     Region          43..69
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320514"
     Region          76..101
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320514"
     Site            order(98,101..102,115..120,122..123,126,169,171..175,203,
                     206..208,210..212,214..215,272,275..276,278..279,282,
                     294..295,297..299,302,305..306)
                     /site_type="other"
                     /note="putative peptide ligand binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:320514"
     Region          115..145
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320514"
     Region          156..177
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320514"
     Region          203..232
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320514"
     Region          252..282
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320514"
     Region          295..320
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320514"
     CDS             1..361
                     /gene="LOC144115009"
                     /coded_by="XM_077649122.1:5..1090"
                     /db_xref="GeneID:144115009"
ORIGIN      
        1 mdgveesrya eeeaggtnss wandseagsc evspevpqgi qalmylmyia vsiaaiggng
       61 ivcyiviayq rmrtvtnmfi mnlaigdilm aclcipftfv snlllgywpf ggvmcvlvty
      121 aqcvtvfisa ytliaisvdr ytaivyplrp rmtklrskli ialvwivalv tplptalvtq
      181 lvphvcanst yycqeqwgrp eqtavysmal milqyffpll vliftytria vvvwgkqtpg
      241 eaqdardqrm aaskrkmvkm miacvaafll cwlplnlfiv vseqypsiyn lsgigymwfv
      301 chwlamshac ynpliyfwmn akfraglqal frclpasrrr sscfvstcnv kkgaaghsna
      361 i
//
LOCUS       XP_077505249             549 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115010 [Amblyomma americanum].
ACCESSION   XP_077505249
VERSION     XP_077505249.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649123.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..549
                     /product="uncharacterized protein LOC144115010"
                     /calculated_mol_wt=61849
     Region          <19..>446
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..549
                     /gene="LOC144115010"
                     /coded_by="XM_077649123.1:31..1680"
                     /db_xref="GeneID:144115010"
ORIGIN      
        1 mdctkkklkd ayahmvsirk twskknrqla telesckkel diklqvcena easnqvlrgr
       61 lndlqlriva ltaenaqcrl nmqhlveehs nvladnkslv lqhrtekeek dqliaqlrml
      121 asklcdlsdl ddgsalklfe algsalqqls dertslndre raakttieal tgerdglmae
      181 nrvlaekqkd ynelqqakes ledhltihkd vaknitrevh hqltsamdtl kqviqesaia
      241 qgpfeatlqt vvreavrlrt qislfydhws rsqrvtsdms delarkkeev qrlsaecarm
      301 dsvireqmae ieglcsgitn feqlaadsag dlarvqaeak slmeslaaaq meaeereeaa
      361 llllkkdtqi aqlegelqek reelaslvqe kaaaadeser freanqnlke ivfslskqle
      421 qeqirresmd dfamqteeev vkvtdavqtd gclvpkadce vqtsgnddel llqlessehe
      481 vnllkssihr icldnaalgs mykerrgqlt kaseichale dqmkrlaeel kaaktdkeal
      541 qllpvlpme
//
LOCUS       XP_077505250             112 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115011 [Amblyomma americanum].
ACCESSION   XP_077505250
VERSION     XP_077505250.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649124.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..112
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..112
                     /product="uncharacterized protein LOC144115011"
                     /calculated_mol_wt=11715
     CDS             1..112
                     /gene="LOC144115011"
                     /coded_by="XM_077649124.1:286..624"
                     /db_xref="GeneID:144115011"
ORIGIN      
        1 msygypqaph qyqppaapgf apppaysgyp ppppapvaep yfaasapvgd tyqpppppgp
       61 qhlyppqqqg gsyyvppppp ttvivkekdd ncakdcclca lcmglcclcc qd
//
LOCUS       XP_077505251             112 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115011 [Amblyomma americanum].
ACCESSION   XP_077505251
VERSION     XP_077505251.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649125.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..112
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..112
                     /product="uncharacterized protein LOC144115011"
                     /calculated_mol_wt=11715
     CDS             1..112
                     /gene="LOC144115011"
                     /coded_by="XM_077649125.1:168..506"
                     /db_xref="GeneID:144115011"
ORIGIN      
        1 msygypqaph qyqppaapgf apppaysgyp ppppapvaep yfaasapvgd tyqpppppgp
       61 qhlyppqqqg gsyyvppppp ttvivkekdd ncakdcclca lcmglcclcc qd
//
LOCUS       XP_077505252             465 aa            linear   INV 25-NOV-2025
DEFINITION  alpha-(1,3)-fucosyltransferase 10 [Amblyomma americanum].
ACCESSION   XP_077505252
VERSION     XP_077505252.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649126.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..465
                     /product="alpha-(1,3)-fucosyltransferase 10"
                     /calculated_mol_wt=53226
     Region          68..167
                     /region_name="Glyco_tran_10_N"
                     /note="Fucosyltransferase, N-terminal; pfam17039"
                     /db_xref="CDD:465336"
     Region          199..394
                     /region_name="Glyco_transf_10"
                     /note="Glycosyltransferase family 10 (fucosyltransferase)
                     C-term; pfam00852"
                     /db_xref="CDD:459962"
     CDS             1..465
                     /gene="FucTB"
                     /coded_by="XM_077649126.1:27..1424"
                     /db_xref="GeneID:144115014"
ORIGIN      
        1 mvavcaanif rsrrcvvrcs lilltlalls yvsiylllse aesdtllygh yrsdhshytg
       61 vpenvvdpii lwwtpftkqa ghskacgsrr cfftedrvff nhtnlqavif ygseleigdl
      121 plprknnhdw allheespkn nfvfcmpnil pwfnhtatfr rssdlpltlq ylndiadlts
      181 keffvetask etvktnlslv ayvqsdcntp ierdafvfel mkyisvdsyg kcihnkdlpk
      241 elasameamd nkhfwhllan ykfhiaieny acedyitekl wrplivgsvp iyfgspsvvd
      301 wlpnnvdsai lvdnysgped lakaikkidg nsqlyesflg hkihqrisnk allnamkkrr
      361 wgmndakkps fveefeclvc srvwkrlesp ksvnfvanas hyscdpphsm lkkqsrgfwr
      421 elyrqaktea qvledilqan vaideqylqn kilsslqahs ssrgn
//
LOCUS       XP_077505254             554 aa            linear   INV 25-NOV-2025
DEFINITION  ras GTPase-activating protein-binding protein 2 [Amblyomma
            americanum].
ACCESSION   XP_077505254
VERSION     XP_077505254.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649128.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..554
                     /product="ras GTPase-activating protein-binding protein 2"
                     /calculated_mol_wt=58592
     Region          11..129
                     /region_name="NTF2"
                     /note="Nuclear transport factor 2 (NTF2) domain plays an
                     important role in the trafficking of macromolecules, ions
                     and small molecules between the cytoplasm and nucleus.
                     This bi-directional transport of macromolecules across the
                     nuclear envelope requires many...; cd00780"
                     /db_xref="CDD:238403"
     Site            order(35,41,52,77,79..80,89,111,122..124,126,128)
                     /site_type="active"
                     /note="TAP/p15 interaction [active]"
                     /db_xref="CDD:238403"
     Site            order(39,41..42,74..75,77,79..80,87,91..93,97,99..102,105,
                     111,122..124,126..129)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:238403"
     Site            order(42..43,64,69,92,94,96..97,99,102,125,127,129)
                     /site_type="active"
                     /note="RanGDP-NTF2 interaction [active]"
                     /db_xref="CDD:238403"
     Region          <312..>402
                     /region_name="PHA03381"
                     /note="tegument protein VP22; Provisional"
                     /db_xref="CDD:177618"
     Region          401..489
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..554
                     /gene="rin"
                     /coded_by="XM_077649128.1:86..1750"
                     /db_xref="GeneID:144115015"
ORIGIN      
        1 mvmetptalh igrefvrqyy tvlnktplhl hrfysqdssf vhggpekqec vmgqqeihqr
       61 imqlnfrdch akikqvdslt tlgegvviqv tgelsnagqp mrrfmqtfvl apqqplkyyv
      121 rndifryqde vfteeeeeee galagqqaqe eapeqlmahh qsahtdvaqh paepttplvn
      181 etpvpprseq lgnggaspss nsvtsgsgrp ayfepqvmrn gtahlasege vssqaslass
      241 sppdgpssap tapagntmnw kddeapappa apqvnhqapp etktyanmvs knsaplsssg
      301 ftslspaapf ggtptsgagh gpsgaghpgg rfggdhlpgg lpprpdqrgg prpqqqarpq
      361 rsslppapkr teggrndavl sggddgaapp plrsgtkpqy pdnqqvfvgn lphtiteeqv
      421 recfehfgrv lefrinsktt gkmttggkav pncgfvifes peavqallqr vaaepifinn
      481 trvnveekkt kqklategrg gsfssgsprg gaaggpmqrg ppggrtngag grggafsrgg
      541 grnppggglg irhn
//
LOCUS       XP_077505255             554 aa            linear   INV 25-NOV-2025
DEFINITION  ras GTPase-activating protein-binding protein 2 [Amblyomma
            americanum].
ACCESSION   XP_077505255
VERSION     XP_077505255.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649129.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..554
                     /product="ras GTPase-activating protein-binding protein 2"
                     /calculated_mol_wt=58592
     Region          11..129
                     /region_name="NTF2"
                     /note="Nuclear transport factor 2 (NTF2) domain plays an
                     important role in the trafficking of macromolecules, ions
                     and small molecules between the cytoplasm and nucleus.
                     This bi-directional transport of macromolecules across the
                     nuclear envelope requires many...; cd00780"
                     /db_xref="CDD:238403"
     Site            order(35,41,52,77,79..80,89,111,122..124,126,128)
                     /site_type="active"
                     /note="TAP/p15 interaction [active]"
                     /db_xref="CDD:238403"
     Site            order(39,41..42,74..75,77,79..80,87,91..93,97,99..102,105,
                     111,122..124,126..129)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:238403"
     Site            order(42..43,64,69,92,94,96..97,99,102,125,127,129)
                     /site_type="active"
                     /note="RanGDP-NTF2 interaction [active]"
                     /db_xref="CDD:238403"
     Region          <312..>402
                     /region_name="PHA03381"
                     /note="tegument protein VP22; Provisional"
                     /db_xref="CDD:177618"
     Region          401..489
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..554
                     /gene="rin"
                     /coded_by="XM_077649129.1:148..1812"
                     /db_xref="GeneID:144115015"
ORIGIN      
        1 mvmetptalh igrefvrqyy tvlnktplhl hrfysqdssf vhggpekqec vmgqqeihqr
       61 imqlnfrdch akikqvdslt tlgegvviqv tgelsnagqp mrrfmqtfvl apqqplkyyv
      121 rndifryqde vfteeeeeee galagqqaqe eapeqlmahh qsahtdvaqh paepttplvn
      181 etpvpprseq lgnggaspss nsvtsgsgrp ayfepqvmrn gtahlasege vssqaslass
      241 sppdgpssap tapagntmnw kddeapappa apqvnhqapp etktyanmvs knsaplsssg
      301 ftslspaapf ggtptsgagh gpsgaghpgg rfggdhlpgg lpprpdqrgg prpqqqarpq
      361 rsslppapkr teggrndavl sggddgaapp plrsgtkpqy pdnqqvfvgn lphtiteeqv
      421 recfehfgrv lefrinsktt gkmttggkav pncgfvifes peavqallqr vaaepifinn
      481 trvnveekkt kqklategrg gsfssgsprg gaaggpmqrg ppggrtngag grggafsrgg
      541 grnppggglg irhn
//
LOCUS       XP_077505256             554 aa            linear   INV 25-NOV-2025
DEFINITION  ras GTPase-activating protein-binding protein 2 [Amblyomma
            americanum].
ACCESSION   XP_077505256
VERSION     XP_077505256.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649130.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..554
                     /product="ras GTPase-activating protein-binding protein 2"
                     /calculated_mol_wt=58592
     Region          11..129
                     /region_name="NTF2"
                     /note="Nuclear transport factor 2 (NTF2) domain plays an
                     important role in the trafficking of macromolecules, ions
                     and small molecules between the cytoplasm and nucleus.
                     This bi-directional transport of macromolecules across the
                     nuclear envelope requires many...; cd00780"
                     /db_xref="CDD:238403"
     Site            order(35,41,52,77,79..80,89,111,122..124,126,128)
                     /site_type="active"
                     /note="TAP/p15 interaction [active]"
                     /db_xref="CDD:238403"
     Site            order(39,41..42,74..75,77,79..80,87,91..93,97,99..102,105,
                     111,122..124,126..129)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:238403"
     Site            order(42..43,64,69,92,94,96..97,99,102,125,127,129)
                     /site_type="active"
                     /note="RanGDP-NTF2 interaction [active]"
                     /db_xref="CDD:238403"
     Region          <312..>402
                     /region_name="PHA03381"
                     /note="tegument protein VP22; Provisional"
                     /db_xref="CDD:177618"
     Region          401..489
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..554
                     /gene="rin"
                     /coded_by="XM_077649130.1:264..1928"
                     /db_xref="GeneID:144115015"
ORIGIN      
        1 mvmetptalh igrefvrqyy tvlnktplhl hrfysqdssf vhggpekqec vmgqqeihqr
       61 imqlnfrdch akikqvdslt tlgegvviqv tgelsnagqp mrrfmqtfvl apqqplkyyv
      121 rndifryqde vfteeeeeee galagqqaqe eapeqlmahh qsahtdvaqh paepttplvn
      181 etpvpprseq lgnggaspss nsvtsgsgrp ayfepqvmrn gtahlasege vssqaslass
      241 sppdgpssap tapagntmnw kddeapappa apqvnhqapp etktyanmvs knsaplsssg
      301 ftslspaapf ggtptsgagh gpsgaghpgg rfggdhlpgg lpprpdqrgg prpqqqarpq
      361 rsslppapkr teggrndavl sggddgaapp plrsgtkpqy pdnqqvfvgn lphtiteeqv
      421 recfehfgrv lefrinsktt gkmttggkav pncgfvifes peavqallqr vaaepifinn
      481 trvnveekkt kqklategrg gsfssgsprg gaaggpmqrg ppggrtngag grggafsrgg
      541 grnppggglg irhn
//
LOCUS       XP_077505257             373 aa            linear   INV 25-NOV-2025
DEFINITION  F-box only protein 7-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505257
VERSION     XP_077505257.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649131.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..373
                     /product="F-box only protein 7-like isoform X1"
                     /calculated_mol_wt=42375
     Region          48..184
                     /region_name="PI31_Prot_N"
                     /note="PI31 proteasome regulator N-terminal; pfam11566"
                     /db_xref="CDD:463296"
     Region          204..243
                     /region_name="F-box-like"
                     /note="pfam12937"
                     /db_xref="CDD:463757"
     CDS             1..373
                     /gene="LOC144115016"
                     /coded_by="XM_077649131.1:296..1417"
                     /db_xref="GeneID:144115016"
ORIGIN      
        1 maslvasafg angvtamree prpqycyqvq dftmplpkql edlhmkcnin hprealvlav
       61 hvlmteagfe ldgwqetlpd ggikehpask nfawersdcl mkigyshptc ptvsflvafv
      121 lfdpsvivhg lvmhensteg fslnlripdf iqkaflsdpe pnkvyknlpt lsrlvkdsva
      181 lpllgwaqca tnvaptfgfm tlsseiqlli laylnvrdll aasrvntdlf qlashpvlwr
      241 klffknfpsv evtptdnvsd wrerykreyl lrkeqqehal rllprmqvrl vmpnplrnah
      301 rpfllppvpq lsdddfdfdm lmprpprfsp pyvpqlprrp pdpafprpyf gqysvlprgv
      361 lpnqpfgpgf gfl
//
LOCUS       XP_077505258             340 aa            linear   INV 25-NOV-2025
DEFINITION  F-box only protein 7-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505258
VERSION     XP_077505258.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649132.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..340
                     /product="F-box only protein 7-like isoform X2"
                     /calculated_mol_wt=38754
     Region          15..151
                     /region_name="PI31_Prot_N"
                     /note="PI31 proteasome regulator N-terminal; pfam11566"
                     /db_xref="CDD:463296"
     Region          171..210
                     /region_name="F-box-like"
                     /note="pfam12937"
                     /db_xref="CDD:463757"
     CDS             1..340
                     /gene="LOC144115016"
                     /coded_by="XM_077649132.1:307..1329"
                     /db_xref="GeneID:144115016"
ORIGIN      
        1 mplpkqledl hmkcninhpr ealvlavhvl mteagfeldg wqetlpdggi kehpasknfa
       61 wersdclmki gyshptcptv sflvafvlfd psvivhglvm henstegfsl nlripdfiqk
      121 aflsdpepnk vyknlptlsr lvkdsvalpl lgwaqcatnv aptfgfmtls seiqllilay
      181 lnvrdllaas rvntdlfqla shpvlwrklf fknfpsvevt ptdnvsdwre rykreyllrk
      241 eqqehalrll prmqvrlvmp nplrnahrpf llppvpqlsd ddfdfdmlmp rpprfsppyv
      301 pqlprrppdp afprpyfgqy svlprgvlpn qpfgpgfgfl
//
LOCUS       XP_077505259             350 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115017 [Amblyomma americanum].
ACCESSION   XP_077505259
VERSION     XP_077505259.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649133.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..350
                     /product="uncharacterized protein LOC144115017"
                     /calculated_mol_wt=38513
     Region          245..341
                     /region_name="Spaetzle"
                     /note="cl29083"
                     /db_xref="CDD:475150"
     CDS             1..350
                     /gene="LOC144115017"
                     /coded_by="XM_077649133.1:190..1242"
                     /db_xref="GeneID:144115017"
ORIGIN      
        1 mtlrlvgptl plvllvgaas akagspvrla vwlvpllscc cwgglvlgal palaagaagp
       61 vwravssssy yiadvsfpqs rprrgfvpsp pycpetgrtv caqvddyptd yiyhvllgsk
      121 skhfnlsslf sderlgdaqp nrallpdqpr tdsyvvyqta vhydydhqgg phhhggphhq
      181 ggqqqqhrrp phqqrrqqph qepphhhhhq aqhdaddwad ttivlpptfv yhkrdlvkra
      241 pvvdpacpvr tmfvspkaal sdrsqwkyvv nlqerdpsak qvirvdvcss pdaacsnais
      301 lpfgytsrct qkylkkklls ldsdgsgtsd enffvpsccv celvrdvgkk
//
LOCUS       XP_077505260             659 aa            linear   INV 25-NOV-2025
DEFINITION  heat shock protein 70 cognate 3 [Amblyomma americanum].
ACCESSION   XP_077505260
VERSION     XP_077505260.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649134.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..659
                     /product="heat shock protein 70 cognate 3"
                     /calculated_mol_wt=72507
     Region          36..641
                     /region_name="HSP70"
                     /note="Hsp70 protein; pfam00012"
                     /db_xref="CDD:394970"
     CDS             1..659
                     /gene="Hsc70-3"
                     /coded_by="XM_077649134.1:171..2150"
                     /db_xref="GeneID:144115018"
ORIGIN      
        1 mhkfslclta allvaavvsa kkesdaakdk envgtvigid lgttyscvgv fkngrveiia
       61 ndqgnritps yvaftaeger ligdaaknql tsnpentvfd akrligrdwt dpsvqhdvky
      121 fpfivkekng kphivvkasg kdekvfapee isamvlskmk etaeaylgkk vthavvtvpa
      181 yfndaqrqat kdagtiagln vmriinepta aaiaygldkk egeknilvfd lgggtfdvsl
      241 ltidngvfev vstngdthlg gedfdqrvme hfiklykkkt gqdvrkdnra vqklrrevek
      301 akrtlstahq arieiesffe gedfsetltr akfeelnmdl frstmkpvqk vledgdlkks
      361 dvdeivlvgg stripkvqql vkeffngkep trginpdeav aygaavqagv lggeedtgdl
      421 vlldvnpltl gietvggvmt kliprntvip tkksqifsta sddqstvtiq vyegerpmtk
      481 dnhqlgrfdl tgippaprgv pqievtfeid vngilkvsae dkgtgnkqki visndqnrlt
      541 pediermvkd aekfadedkk vkekvearne lesyayslkn qvedkeklga kledkektki
      601 keaveetikw leqnsdadae elkaqkksle eivqpivakl ysggtppptd kddstkdel
//
LOCUS       XP_077505261             590 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115019 [Amblyomma americanum].
ACCESSION   XP_077505261
VERSION     XP_077505261.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649135.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..590
                     /product="uncharacterized protein LOC144115019"
                     /calculated_mol_wt=65907
     Region          <283..338
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     CDS             1..590
                     /gene="LOC144115019"
                     /coded_by="XM_077649135.1:848..2620"
                     /db_xref="GeneID:144115019"
ORIGIN      
        1 msqtdllnyd rpsatgptrf saesqmeedi evmpeqqqqq vrggpaaaaa gsqalgsstp
       61 atqlsqvstn dgsnadweia msrrqkrrqq rgeqqqvlvg kslgnslgre gapprrrlpp
      121 lprddlkvvl rprglavkdl qthqvaravv aatgqgckae dliirlrngs niiivstdne
      181 aaaqkircit qlsfggksyt vnghvaapeg tlrgvihgvd pgtspeelkt nlrvrtqgvk
      241 ilaarmlgns stavitfdgp alpkqvlyyg gemwcypcrp trqvcyacgq qghrmdvcpi
      301 tetrtcrqcg cqnpeemhqc tpkcllcggg haagtkdckq hlkpaselrw gpqqqqrrsr
      361 srsrgrrprw frddsqgrse srsrshgrsr srgpldrdes fppldnagkp gqrqqqqtkk
      421 sggvkvswed gppkslyaph sntntqtqnn qlmaeiknlr releqscres nelkrqkktm
      481 rtgyspirtp tpppmemqrn nsegsnpndk ietfmtqile rmqqmqneiq qtqqkfalqe
      541 qsfrnyvknq ntqrttndar drlfnerrfg tdtpstpnnn qtwqnannnn
//
LOCUS       XP_077505262             590 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115019 [Amblyomma americanum].
ACCESSION   XP_077505262
VERSION     XP_077505262.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649136.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..590
                     /product="uncharacterized protein LOC144115019"
                     /calculated_mol_wt=65907
     Region          <283..338
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     CDS             1..590
                     /gene="LOC144115019"
                     /coded_by="XM_077649136.1:800..2572"
                     /db_xref="GeneID:144115019"
ORIGIN      
        1 msqtdllnyd rpsatgptrf saesqmeedi evmpeqqqqq vrggpaaaaa gsqalgsstp
       61 atqlsqvstn dgsnadweia msrrqkrrqq rgeqqqvlvg kslgnslgre gapprrrlpp
      121 lprddlkvvl rprglavkdl qthqvaravv aatgqgckae dliirlrngs niiivstdne
      181 aaaqkircit qlsfggksyt vnghvaapeg tlrgvihgvd pgtspeelkt nlrvrtqgvk
      241 ilaarmlgns stavitfdgp alpkqvlyyg gemwcypcrp trqvcyacgq qghrmdvcpi
      301 tetrtcrqcg cqnpeemhqc tpkcllcggg haagtkdckq hlkpaselrw gpqqqqrrsr
      361 srsrgrrprw frddsqgrse srsrshgrsr srgpldrdes fppldnagkp gqrqqqqtkk
      421 sggvkvswed gppkslyaph sntntqtqnn qlmaeiknlr releqscres nelkrqkktm
      481 rtgyspirtp tpppmemqrn nsegsnpndk ietfmtqile rmqqmqneiq qtqqkfalqe
      541 qsfrnyvknq ntqrttndar drlfnerrfg tdtpstpnnn qtwqnannnn
//
LOCUS       XP_077505263             590 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115019 [Amblyomma americanum].
ACCESSION   XP_077505263
VERSION     XP_077505263.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649137.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..590
                     /product="uncharacterized protein LOC144115019"
                     /calculated_mol_wt=65907
     Region          <283..338
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     CDS             1..590
                     /gene="LOC144115019"
                     /coded_by="XM_077649137.1:1038..2810"
                     /db_xref="GeneID:144115019"
ORIGIN      
        1 msqtdllnyd rpsatgptrf saesqmeedi evmpeqqqqq vrggpaaaaa gsqalgsstp
       61 atqlsqvstn dgsnadweia msrrqkrrqq rgeqqqvlvg kslgnslgre gapprrrlpp
      121 lprddlkvvl rprglavkdl qthqvaravv aatgqgckae dliirlrngs niiivstdne
      181 aaaqkircit qlsfggksyt vnghvaapeg tlrgvihgvd pgtspeelkt nlrvrtqgvk
      241 ilaarmlgns stavitfdgp alpkqvlyyg gemwcypcrp trqvcyacgq qghrmdvcpi
      301 tetrtcrqcg cqnpeemhqc tpkcllcggg haagtkdckq hlkpaselrw gpqqqqrrsr
      361 srsrgrrprw frddsqgrse srsrshgrsr srgpldrdes fppldnagkp gqrqqqqtkk
      421 sggvkvswed gppkslyaph sntntqtqnn qlmaeiknlr releqscres nelkrqkktm
      481 rtgyspirtp tpppmemqrn nsegsnpndk ietfmtqile rmqqmqneiq qtqqkfalqe
      541 qsfrnyvknq ntqrttndar drlfnerrfg tdtpstpnnn qtwqnannnn
//
LOCUS       XP_077505264             590 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115019 [Amblyomma americanum].
ACCESSION   XP_077505264
VERSION     XP_077505264.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649138.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..590
                     /product="uncharacterized protein LOC144115019"
                     /calculated_mol_wt=65907
     Region          <283..338
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     CDS             1..590
                     /gene="LOC144115019"
                     /coded_by="XM_077649138.1:924..2696"
                     /db_xref="GeneID:144115019"
ORIGIN      
        1 msqtdllnyd rpsatgptrf saesqmeedi evmpeqqqqq vrggpaaaaa gsqalgsstp
       61 atqlsqvstn dgsnadweia msrrqkrrqq rgeqqqvlvg kslgnslgre gapprrrlpp
      121 lprddlkvvl rprglavkdl qthqvaravv aatgqgckae dliirlrngs niiivstdne
      181 aaaqkircit qlsfggksyt vnghvaapeg tlrgvihgvd pgtspeelkt nlrvrtqgvk
      241 ilaarmlgns stavitfdgp alpkqvlyyg gemwcypcrp trqvcyacgq qghrmdvcpi
      301 tetrtcrqcg cqnpeemhqc tpkcllcggg haagtkdckq hlkpaselrw gpqqqqrrsr
      361 srsrgrrprw frddsqgrse srsrshgrsr srgpldrdes fppldnagkp gqrqqqqtkk
      421 sggvkvswed gppkslyaph sntntqtqnn qlmaeiknlr releqscres nelkrqkktm
      481 rtgyspirtp tpppmemqrn nsegsnpndk ietfmtqile rmqqmqneiq qtqqkfalqe
      541 qsfrnyvknq ntqrttndar drlfnerrfg tdtpstpnnn qtwqnannnn
//
LOCUS       XP_077505265             590 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115019 [Amblyomma americanum].
ACCESSION   XP_077505265
VERSION     XP_077505265.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649139.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..590
                     /product="uncharacterized protein LOC144115019"
                     /calculated_mol_wt=65907
     Region          <283..338
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     CDS             1..590
                     /gene="LOC144115019"
                     /coded_by="XM_077649139.1:680..2452"
                     /db_xref="GeneID:144115019"
ORIGIN      
        1 msqtdllnyd rpsatgptrf saesqmeedi evmpeqqqqq vrggpaaaaa gsqalgsstp
       61 atqlsqvstn dgsnadweia msrrqkrrqq rgeqqqvlvg kslgnslgre gapprrrlpp
      121 lprddlkvvl rprglavkdl qthqvaravv aatgqgckae dliirlrngs niiivstdne
      181 aaaqkircit qlsfggksyt vnghvaapeg tlrgvihgvd pgtspeelkt nlrvrtqgvk
      241 ilaarmlgns stavitfdgp alpkqvlyyg gemwcypcrp trqvcyacgq qghrmdvcpi
      301 tetrtcrqcg cqnpeemhqc tpkcllcggg haagtkdckq hlkpaselrw gpqqqqrrsr
      361 srsrgrrprw frddsqgrse srsrshgrsr srgpldrdes fppldnagkp gqrqqqqtkk
      421 sggvkvswed gppkslyaph sntntqtqnn qlmaeiknlr releqscres nelkrqkktm
      481 rtgyspirtp tpppmemqrn nsegsnpndk ietfmtqile rmqqmqneiq qtqqkfalqe
      541 qsfrnyvknq ntqrttndar drlfnerrfg tdtpstpnnn qtwqnannnn
//
LOCUS       XP_077505266             590 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115019 [Amblyomma americanum].
ACCESSION   XP_077505266
VERSION     XP_077505266.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649140.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..590
                     /product="uncharacterized protein LOC144115019"
                     /calculated_mol_wt=65907
     Region          <283..338
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     CDS             1..590
                     /gene="LOC144115019"
                     /coded_by="XM_077649140.1:847..2619"
                     /db_xref="GeneID:144115019"
ORIGIN      
        1 msqtdllnyd rpsatgptrf saesqmeedi evmpeqqqqq vrggpaaaaa gsqalgsstp
       61 atqlsqvstn dgsnadweia msrrqkrrqq rgeqqqvlvg kslgnslgre gapprrrlpp
      121 lprddlkvvl rprglavkdl qthqvaravv aatgqgckae dliirlrngs niiivstdne
      181 aaaqkircit qlsfggksyt vnghvaapeg tlrgvihgvd pgtspeelkt nlrvrtqgvk
      241 ilaarmlgns stavitfdgp alpkqvlyyg gemwcypcrp trqvcyacgq qghrmdvcpi
      301 tetrtcrqcg cqnpeemhqc tpkcllcggg haagtkdckq hlkpaselrw gpqqqqrrsr
      361 srsrgrrprw frddsqgrse srsrshgrsr srgpldrdes fppldnagkp gqrqqqqtkk
      421 sggvkvswed gppkslyaph sntntqtqnn qlmaeiknlr releqscres nelkrqkktm
      481 rtgyspirtp tpppmemqrn nsegsnpndk ietfmtqile rmqqmqneiq qtqqkfalqe
      541 qsfrnyvknq ntqrttndar drlfnerrfg tdtpstpnnn qtwqnannnn
//
LOCUS       XP_077505267             590 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115019 [Amblyomma americanum].
ACCESSION   XP_077505267
VERSION     XP_077505267.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649141.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..590
                     /product="uncharacterized protein LOC144115019"
                     /calculated_mol_wt=65907
     Region          <283..338
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     CDS             1..590
                     /gene="LOC144115019"
                     /coded_by="XM_077649141.1:1698..3470"
                     /db_xref="GeneID:144115019"
ORIGIN      
        1 msqtdllnyd rpsatgptrf saesqmeedi evmpeqqqqq vrggpaaaaa gsqalgsstp
       61 atqlsqvstn dgsnadweia msrrqkrrqq rgeqqqvlvg kslgnslgre gapprrrlpp
      121 lprddlkvvl rprglavkdl qthqvaravv aatgqgckae dliirlrngs niiivstdne
      181 aaaqkircit qlsfggksyt vnghvaapeg tlrgvihgvd pgtspeelkt nlrvrtqgvk
      241 ilaarmlgns stavitfdgp alpkqvlyyg gemwcypcrp trqvcyacgq qghrmdvcpi
      301 tetrtcrqcg cqnpeemhqc tpkcllcggg haagtkdckq hlkpaselrw gpqqqqrrsr
      361 srsrgrrprw frddsqgrse srsrshgrsr srgpldrdes fppldnagkp gqrqqqqtkk
      421 sggvkvswed gppkslyaph sntntqtqnn qlmaeiknlr releqscres nelkrqkktm
      481 rtgyspirtp tpppmemqrn nsegsnpndk ietfmtqile rmqqmqneiq qtqqkfalqe
      541 qsfrnyvknq ntqrttndar drlfnerrfg tdtpstpnnn qtwqnannnn
//
LOCUS       XP_077505268             819 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115021 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505268
VERSION     XP_077505268.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649142.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..819
                     /product="uncharacterized protein LOC144115021 isoform X1"
                     /calculated_mol_wt=91950
     Region          98..335
                     /region_name="GBP"
                     /note="Guanylate-binding protein (GBP) family (N-terminal
                     domain); cd01851"
                     /db_xref="CDD:206650"
     Site            111..118
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206650"
     Site            order(113..119,133..135,140..141,170,251..252,300)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206650"
     Site            order(130..135,140..147)
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206650"
     Site            141
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206650"
     Site            167..170
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206650"
     Site            order(169..173,183..189,192..194)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206650"
     Site            251..254
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206650"
     Site            298..300
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206650"
     CDS             1..819
                     /gene="LOC144115021"
                     /coded_by="XM_077649142.1:40..2499"
                     /db_xref="GeneID:144115021"
ORIGIN      
        1 msnyasvamv vevwicagsr nhsgvmtvar gvreamwaac lkcvarfall mlllvflcsp
       61 vtasfqsfts ndapiqivkp sadhkklivd denikaiski kgpvatvgvv gkfhsgksfl
      121 mnqlmgktkg fgigpsvrpe tmgiwmwgep lkvklpsgqq lslifldteg faatnvseny
      181 dakifaiatl isshliynsv kiidqaeidy lellarrtql falrsqlska kwngefvhdl
      241 lsfppllwvv qdfvqttvnn etptqwlhrl meahsresee ykislldvfk slechtlfip
      301 avkrtlltdl sqaseedltd eyieernala kklyevlvpk tkngkpitgt elagllrvlv
      361 daandgslad vpsrwsafve rlmnsaaedc aqiyisemns llekhdhgai rdddfmarht
      421 eakkmsftll eqllhglpda ldtassqltk qldnefslrr emnfkklklf clslqekfll
      481 daegymksip vpttsatlka tvnsyldqll etfatklsfl vpkeetsvys nslkkslehl
      541 vaavqlkndk alerlfensi aaaadvfssk vtlqgalsds qferlkktgv daafevfdss
      601 cknfsnekay eaheallktt lskaieqlkk dnerllqkhm ietvktllik feektgpdhl
      661 tlpmnvsdle irlniertnv eaeftvdfed fhtsphysqy fkeltlrlas ivderqkenv
      721 kafgqvvdep lkrarqiill sapkykteyg lrsyimqvcl lqleegkaky wqedlkknii
      781 vdfisgdpel snalasvrgl wssilgffaw vlslfgvdl
//
LOCUS       XP_077505269             811 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115021 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505269
VERSION     XP_077505269.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649143.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..811
                     /product="uncharacterized protein LOC144115021 isoform X2"
                     /calculated_mol_wt=91126
     Region          90..327
                     /region_name="GBP"
                     /note="Guanylate-binding protein (GBP) family (N-terminal
                     domain); cd01851"
                     /db_xref="CDD:206650"
     Site            103..110
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206650"
     Site            order(105..111,125..127,132..133,162,243..244,292)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206650"
     Site            order(122..127,132..139)
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206650"
     Site            133
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206650"
     Site            159..162
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206650"
     Site            order(161..165,175..181,184..186)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206650"
     Site            243..246
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206650"
     Site            290..292
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206650"
     CDS             1..811
                     /gene="LOC144115021"
                     /coded_by="XM_077649143.1:210..2645"
                     /db_xref="GeneID:144115021"
ORIGIN      
        1 mvvevwicag srnhsgvmtv argvreamwa aclkcvarfa llmlllvflc spvtasfqsf
       61 tsndapiqiv kpsadhkkli vddenikais kikgpvatvg vvgkfhsgks flmnqlmgkt
      121 kgfgigpsvr petmgiwmwg eplkvklpsg qqlslifldt egfaatnvse nydakifaia
      181 tlisshliyn svkiidqaei dylellarrt qlfalrsqls kakwngefvh dllsfppllw
      241 vvqdfvqttv nnetptqwlh rlmeahsres eeykislldv fkslechtlf ipavkrtllt
      301 dlsqaseedl tdeyieerna lakklyevlv pktkngkpit gtelagllrv lvdaandgsl
      361 advpsrwsaf verlmnsaae dcaqiyisem nsllekhdhg airdddfmar hteakkmsft
      421 lleqllhglp daldtassql tkqldnefsl rremnfkklk lfclslqekf lldaegymks
      481 ipvpttsatl katvnsyldq lletfatkls flvpkeetsv ysnslkksle hlvaavqlkn
      541 dkalerlfen siaaaadvfs skvtlqgals dsqferlkkt gvdaafevfd sscknfsnek
      601 ayeaheallk ttlskaieql kkdnerllqk hmietvktll ikfeektgpd hltlpmnvsd
      661 leirlniert nveaeftvdf edfhtsphys qyfkeltlrl asivderqke nvkafgqvvd
      721 eplkrarqii llsapkykte yglrsyimqv cllqleegka kywqedlkkn iivdfisgdp
      781 elsnalasvr glwssilgff awvlslfgvd l
//
LOCUS       XP_077505270             149 aa            linear   INV 25-NOV-2025
DEFINITION  oligosaccharyltransferase complex subunit ostc-A isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077505270
VERSION     XP_077505270.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649144.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..149
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..149
                     /product="oligosaccharyltransferase complex subunit ostc-A
                     isoform X1"
                     /calculated_mol_wt=16723
     Region          <29..146
                     /region_name="OST3_OST6"
                     /note="OST3 / OST6 family, transporter family; pfam04756"
                     /db_xref="CDD:461420"
     CDS             1..149
                     /gene="LOC144115022"
                     /coded_by="XM_077649144.1:48..497"
                     /db_xref="GeneID:144115022"
ORIGIN      
        1 meflyglpfy vlevpnlklk rptwfhqpsp mvvyslvlfs yflvcggiiy dviveppsig
       61 stvdehghsk pvafmpyrvn gqyimeglas sflftvgglg fvildrthnp stprlnrill
      121 istgficilv sfitcyvfmr iklpgylha
//
LOCUS       XP_077505271             136 aa            linear   INV 25-NOV-2025
DEFINITION  oligosaccharyltransferase complex subunit OSTC isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077505271
VERSION     XP_077505271.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649145.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..136
                     /product="oligosaccharyltransferase complex subunit OSTC
                     isoform X2"
                     /calculated_mol_wt=15327
     CDS             1..136
                     /gene="LOC144115022"
                     /coded_by="XM_077649145.1:50..460"
                     /db_xref="GeneID:144115022"
ORIGIN      
        1 meflyglpfy vlevpnlklk rptwfhqpsp mvvyslvlfs yflvcggiiy dviveppsig
       61 stvdehghsk pvafmpyrvn gqyimeglas sflftwilac lilhlpakci qdhwkasssr
      121 aikfslfkci avchsi
//
LOCUS       XP_077505272            5446 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase-like protein poe isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505272
VERSION     XP_077505272.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649146.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..5446
                     /product="E3 ubiquitin-protein ligase-like protein poe
                     isoform X1"
                     /calculated_mol_wt=595914
     Region          54..1767
                     /region_name="E3_UBR4_N"
                     /note="E3 ubiquitin-protein ligase UBR4 N-terminal;
                     pfam19423"
                     /db_xref="CDD:466074"
     Region          1777..1847
                     /region_name="UBR-box_UBR4"
                     /note="UBR-box found in RING-type E3 ubiquitin-protein
                     ligase UBR4 and similar proteins; cd19680"
                     /db_xref="CDD:439078"
     Site            order(1796,1799,1808,1811,1815..1816,1819,1831,1833,1841)
                     /site_type="other"
                     /note="putative Zn binding site [ion binding]"
                     /db_xref="CDD:439078"
     Region          3855..3899
                     /region_name="DZR"
                     /note="Double zinc ribbon; pfam12773"
                     /db_xref="CDD:432773"
     Region          4638..5426
                     /region_name="E3_UbLigase_R4"
                     /note="E3 ubiquitin-protein ligase UBR4; pfam13764"
                     /db_xref="CDD:433459"
     CDS             1..5446
                     /gene="poe"
                     /coded_by="XM_077649146.1:51..16391"
                     /db_xref="GeneID:144115023"
ORIGIN      
        1 masassgpdw fsnavtilts nltslnksdl qiiiksviqs esvllaeeed crefreafva
       61 lsahfinasa tslsrsqivt aaqacsillk vllkclkahn ansllqqnli mvligglcis
      121 nagtpiksel stlssalksa qlpsslaskp dgaverrrdd trelrhlwln pstcileqls
      181 tpvldnamgp ksdvsadldk dldlsashii dpgmeaknfl laknvaslqr eragdilldv
      241 clalpsvvqy sqgledvlls cgtmaldcqt snmvltgska lfadillvck clglpvlepl
      301 gearlkrlvr lalgcghmav avtqavcvlg vsstgggsqp asgatkgvpc wddeghmala
      361 ahvveacldi ynviastmrs ssragghvlq nlhvltswll lrglqailgl ntfldraack
      421 egagtkgnrg gldqtptrtr dqsaglkqnl lriqqgfgvl pvalanqvls lltsifgelr
      481 leagaaleea drgagpaeer ivifldnint pfsawkcvrv lmqlnlpall fdlvsvsyrk
      541 agmlrriqkh ptdgdnfsts dsntfyeedf stsddssade aagpktsgql gessghprts
      601 lhkkrarssn lqtggrshpr ktnagqvfqe vrdddsepil ghwyeqtiap rehspgpgal
      661 qpkgapetev penrgvrcsl adgrplipdk eephgyiqls skvinflnay lvnfepvqgy
      721 lksgigtpqm vilagiikdl dretckssig llfgaslgqm yeefskalar fihnilatgl
      781 lddvyqdgll thlninpwtq kewplqiywr tlsvlaqvll lrlqkdkddl rsendtacil
      841 ifrlvlntmq kailsshvdd nedlnvehtq lllflfhnlq lmqkkvvlls vaktiinvap
      901 vsqsplkdvq iiyiarllhi feyliknlyd apaalvdqvq anlftlhgsa cispdtetak
      961 qhgklffpck eveenfaknt lsdvvqgahi rpkfyhlmil esgtqdvpkl dgfacsflla
     1021 spdvlnysdl ygavlsllwi gaqcdavkrp sdypklsylg aaavqycfga vwrlllslpp
     1081 siqlsealat gnamldlpqi lhaivwvsrf ghknfniwik vssafcmlpc tfrsrpillh
     1141 dlhlrrrsfp slklllrwkl saadslvkqg mtaqraestl hqvtanasql lfdvrvfkql
     1201 askqlgqgpa dlsgpvsrse lphlmdlfvl ealvaklqvs ldvtllkpsn eshsrevkef
     1261 sweifpillr liklyntcir wslllqavel sptgrsetsl ramdvvlrmt srrcsqvsst
     1321 vaaivshlpp qvdstldfwn amavnvcnwr ndfandslps esyiaavqva hftplsanst
     1381 fsinsslkri lqcivrfaqd vyswsaedkf sndllramlp llldattesq adfvmlaleg
     1441 iigssdteef aqhvyeemla ncyellinys hkeseinelv fheclkfmes lldkqpgkka
     1501 lekffcdkdi wnillsaaeg nlspaygtrv lkffsklftq idknvgdpsl eklnatlckm
     1561 nrlneldpks lqnwltkvvv gskaancsed gspvqenrnl lqnlttyivk ennkvstqva
     1621 gsfltaliei gtqllspase gvgfsdlmvv mstlasagsg sghvqlfmaa tdwletckky
     1681 lsqkdvlekl eanvssgkhq vimnsacyll syvadlvgsl kllaerpliv grlgaasppl
     1741 dgdaqhpepd sdwadelgpe ddesggedsd edslcnklct ftmtqkefmn qhwyhchtcr
     1801 mvdgvgvcti cakvchkdhd vtyakfgsff cdcgakedgs cqalvkrnpq mlsdtgcgaa
     1861 sttaafslas envlpssirr rassphqggg tgantadgrp paasadskka cdetlkhrqq
     1921 lakkleafrg vliehivstn lvatvleliq flmpaivety nqnspigsst raqkalaelh
     1981 tqekmfehvd qlmvptlgsq egafenvrmn fsgeqgqtir qllsahmirr vamcclaspq
     2041 gkrqhlavsh ekgkitllql sallkqadss krkltitrla sapvpftvls itgnpcnedf
     2101 lavcglkdch vltfsssgsv sehlvlhpql etgnyiikam wlpgsqtela lvtadfvkvy
     2161 dlsvdalnaq fffllptgrl rdccfvcpee gprhlllmss agyvycqpli eessarhgpf
     2221 yvtsvldiqh pdikdsvgtv agggvsiyys hvlqllflsy angksfmacm pqitseitdp
     2281 vliqlkpsag nnggsggttt tsagtnaatg attttvttas ntsggsgsas skptpqplcq
     2341 wsevpnhpgl ilalmqfsnn plvlmvkpgt vlvqeikilt akskitdlva irhptssgem
     2401 rttlillced gslriymasq eqtgywltpa fqptaivglt gvtpaglssg atavgsatpr
     2461 psrrkkslrg ragpstlpms fpvdffehct piqdvefggn dllqiynaqq vkhrlnttgm
     2521 yvactkpggl tleitntdtt mvmvgvrvlv gsqdvqraps tvelfgrshp liltrsrwyd
     2581 lpfsreesls adkkinlffg pssdpscvtm vdsvkvygks kesfgwpees defatgvgia
     2641 aqsntvaatg idpdgipsap lpltaldrlt ssclemlddc fslsslseew lsggakgsal
     2701 dvatrlltlp tppavhqqvr allsalhpsr taytshrdqa llsyvlecln nsskekppfq
     2761 dldgeafyrl vaiargvaca rpanlvqfae sheaatgpae gvdemnkeev leerglplkv
     2821 hiatntlpps esshflsila eafwhlhrqr pqnpalapis khglvhieat vqalvevmya
     2881 fvaldmenvg lvaklfarll lcedtvvsfa akqaiirvlr pktrkrkvfi prtalcstpg
     2941 evgtdhadgg kastsgatgv sqslsttsqp lvsmpgssss tlagfdvqaq aslesqpdve
     3001 shfemlesmd avvllggagg agaggafppl ldippdadde tmvelaials lqdqpgqsed
     3061 lhlgalgsag sqgpsqrass legghysdtt asagasddeg staatdgstl rtspaeqqgg
     3121 sgagsesggs gadsmtgepn vsgrssaygd nalpegglar setssvglpp tlpqedpdta
     3181 wglhalrcal ldrllrflpq lrsvggvrai pfmqvvlmls sdldsederd ratldnllsc
     3241 ligeldrnva rvverspsse eqlivmrlls immsrikssa kvsgpsdsac qncstsaava
     3301 lhasgivdhc lqvlgtlleh wksvqntdsd tpggsasstt gppllkphpa spppdmspff
     3361 lrqyvkghan dvfeaypqll temalrlpyq vkkvwsvvps gcvaptfgst wysylceymm
     3421 vqqtpfirrq vrklllflcg skekyrqlrd lhaleshlag artvctsppm gatplpydal
     3481 ilivehlkac aevatsrtan wqrycqhdnt vlpflfqvsf lldegvasii lqllqcalcg
     3541 tallqqpaqp qqgasklhat sgspfkqphr rerdtseepd evegsdeaqc aalaqqvhns
     3601 versllgqfv rsfllecnst avrwqahsli hqlhrhsqps hrealltlmw elwphlpsyg
     3661 rkaaqfvdll gyftlkapqp qweftekala vlhdqnqlls nhpnsslyns irglvemdgy
     3721 ylesepclvc nnpevayani klsaikvdsr fttstqivkl vgshtvsriv lrigdlkrsk
     3781 mvrainiyyn nrsvqsvvel knrpamwhra krcslaagqt elkmdfplpi vacnlmieya
     3841 dfyenvqass etlqcprcsa svpatpgvca ncgenvfqch kcrainydek dpflcnscgf
     3901 ckyakfdytl takpccavdp iegeedrkka vasinsllek adkvyrqlma hkpalellll
     3961 kvqeqslvdr aleeeaaaaa itatgaattg atsatpanta sasgatcsmv nrsiqqlaqk
     4021 ycseckasfd elskivqkil asrkelveye hkqkdsrkaq aatptvaavr pgrgelpspg
     4081 nfsapagnkq ssgrcygcas aavdhcitll ralatnvpyr qllcergliv elvehnlrqg
     4141 wsqvgqlvcl ltrdnpqatd hlnsllmgri tlallrggqp seqpclavvp atrpttaddt
     4201 eaaatppvvv gvpdlpaavr hevallalsl qkedscweqr lrcvmrlflm gissqspivl
     4261 esitlpclkv lqgiikpdpp iskrnkdksi eelatvrpsg mcvavdlkgw ldgdpvqtfs
     4321 awksrclgrp adtlslgggk rqrredvrar flvekyglrw rqlarrggss mrllmllqpp
     4381 qqqpaqglpc qprplqsnwl rqilfnpscr qartvacslv kalcqvpsrc remldlltty
     4441 lddlgtages gakflalyqs liapdhwkhy laikgllprl gnlitaeieq ltlleettln
     4501 adlsqgfalk mltellasfi evdsirqqyk arlvgcvlng ylslrklvvq rtklvdetqe
     4561 kllalleemt tgteseteaf mavcvetlgr ygledlrtpv fiferlcsli hpeenelgef
     4621 lvtlekdpqq edflqgrmlg npysshepgm gplmrdlknk vcqdcelval leddngmell
     4681 vcnkimsldl pvkevyrkvw cpengeseam rvvyrmrgll gdateefves leakaggpqv
     4741 deeqlyqlar vmgpcgglea mlerldaidd lsrgrplltv llklfglcvk vrsnrlhlle
     4801 pslravacll galrlllgaq eaalaeqlla tleavlaega ahvppqlpeg vtkdditfll
     4861 dqvgtgrpsp rllqllmrvv pfltltdeak mdvlinhfrr qlnfirfdle htpeddvqle
     4921 cfcnlsagie rndngnrlkd llitrgiveg aiqyllvyap paknsllsas enwkeftskp
     4981 alkyvlrilt glsrghektq ilvstecipi ihrleqvssd ehvgslaenl mealkenpnv
     5041 aekieevrkq trdekkrlam amrekqlgel gmhtnekgqv takssilkqm edlgeeaglv
     5101 ciicregykf qpgkllgiyt ftkrcnlddf eakprktpgy stvthfnivh vdchmaavrh
     5161 argrdewesa alqnantkcn gllplwgpqv pesqfascla rhntyiqdct ghldvgymst
     5221 lhdlklllsr fafersfsed sggggpqsnm hlipyllhmv lyvinttrcv areeknlsnf
     5281 lemsperqve ncfesegpcy watmalavws hnrwqygras lvrrmlilah arhlspqgcs
     5341 tlpdmvpref avyrpylcfl gmvdglynim fkkvacstdd gwsvaladyi rhndqlhlel
     5401 gdkllrtfee qvltcqsfre fcdymgpmwe idnpdaflhe alqlrv
//
LOCUS       XP_077505273            5438 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase-like protein poe isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505273
VERSION     XP_077505273.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649147.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..5438
                     /product="E3 ubiquitin-protein ligase-like protein poe
                     isoform X2"
                     /calculated_mol_wt=595147
     Region          54..1767
                     /region_name="E3_UBR4_N"
                     /note="E3 ubiquitin-protein ligase UBR4 N-terminal;
                     pfam19423"
                     /db_xref="CDD:466074"
     Region          1777..1847
                     /region_name="UBR-box_UBR4"
                     /note="UBR-box found in RING-type E3 ubiquitin-protein
                     ligase UBR4 and similar proteins; cd19680"
                     /db_xref="CDD:439078"
     Site            order(1796,1799,1808,1811,1815..1816,1819,1831,1833,1841)
                     /site_type="other"
                     /note="putative Zn binding site [ion binding]"
                     /db_xref="CDD:439078"
     Region          3847..3891
                     /region_name="DZR"
                     /note="Double zinc ribbon; pfam12773"
                     /db_xref="CDD:432773"
     Region          4630..5418
                     /region_name="E3_UbLigase_R4"
                     /note="E3 ubiquitin-protein ligase UBR4; pfam13764"
                     /db_xref="CDD:433459"
     CDS             1..5438
                     /gene="poe"
                     /coded_by="XM_077649147.1:51..16367"
                     /db_xref="GeneID:144115023"
ORIGIN      
        1 masassgpdw fsnavtilts nltslnksdl qiiiksviqs esvllaeeed crefreafva
       61 lsahfinasa tslsrsqivt aaqacsillk vllkclkahn ansllqqnli mvligglcis
      121 nagtpiksel stlssalksa qlpsslaskp dgaverrrdd trelrhlwln pstcileqls
      181 tpvldnamgp ksdvsadldk dldlsashii dpgmeaknfl laknvaslqr eragdilldv
      241 clalpsvvqy sqgledvlls cgtmaldcqt snmvltgska lfadillvck clglpvlepl
      301 gearlkrlvr lalgcghmav avtqavcvlg vsstgggsqp asgatkgvpc wddeghmala
      361 ahvveacldi ynviastmrs ssragghvlq nlhvltswll lrglqailgl ntfldraack
      421 egagtkgnrg gldqtptrtr dqsaglkqnl lriqqgfgvl pvalanqvls lltsifgelr
      481 leagaaleea drgagpaeer ivifldnint pfsawkcvrv lmqlnlpall fdlvsvsyrk
      541 agmlrriqkh ptdgdnfsts dsntfyeedf stsddssade aagpktsgql gessghprts
      601 lhkkrarssn lqtggrshpr ktnagqvfqe vrdddsepil ghwyeqtiap rehspgpgal
      661 qpkgapetev penrgvrcsl adgrplipdk eephgyiqls skvinflnay lvnfepvqgy
      721 lksgigtpqm vilagiikdl dretckssig llfgaslgqm yeefskalar fihnilatgl
      781 lddvyqdgll thlninpwtq kewplqiywr tlsvlaqvll lrlqkdkddl rsendtacil
      841 ifrlvlntmq kailsshvdd nedlnvehtq lllflfhnlq lmqkkvvlls vaktiinvap
      901 vsqsplkdvq iiyiarllhi feyliknlyd apaalvdqvq anlftlhgsa cispdtetak
      961 qhgklffpck eveenfaknt lsdvvqgahi rpkfyhlmil esgtqdvpkl dgfacsflla
     1021 spdvlnysdl ygavlsllwi gaqcdavkrp sdypklsylg aaavqycfga vwrlllslpp
     1081 siqlsealat gnamldlpqi lhaivwvsrf ghknfniwik vssafcmlpc tfrsrpillh
     1141 dlhlrrrsfp slklllrwkl saadslvkqg mtaqraestl hqvtanasql lfdvrvfkql
     1201 askqlgqgpa dlsgpvsrse lphlmdlfvl ealvaklqvs ldvtllkpsn eshsrevkef
     1261 sweifpillr liklyntcir wslllqavel sptgrsetsl ramdvvlrmt srrcsqvsst
     1321 vaaivshlpp qvdstldfwn amavnvcnwr ndfandslps esyiaavqva hftplsanst
     1381 fsinsslkri lqcivrfaqd vyswsaedkf sndllramlp llldattesq adfvmlaleg
     1441 iigssdteef aqhvyeemla ncyellinys hkeseinelv fheclkfmes lldkqpgkka
     1501 lekffcdkdi wnillsaaeg nlspaygtrv lkffsklftq idknvgdpsl eklnatlckm
     1561 nrlneldpks lqnwltkvvv gskaancsed gspvqenrnl lqnlttyivk ennkvstqva
     1621 gsfltaliei gtqllspase gvgfsdlmvv mstlasagsg sghvqlfmaa tdwletckky
     1681 lsqkdvlekl eanvssgkhq vimnsacyll syvadlvgsl kllaerpliv grlgaasppl
     1741 dgdaqhpepd sdwadelgpe ddesggedsd edslcnklct ftmtqkefmn qhwyhchtcr
     1801 mvdgvgvcti cakvchkdhd vtyakfgsff cdcgakedgs cqalvkrnpq mlsdtgcgaa
     1861 sttaafslas envlpssirr rassphqggg tgantadgrp paasadskka cdetlkhrqq
     1921 lakkleafrg vliehivstn lvatvleliq flmpaivety nqnspigsst raqkalaelh
     1981 tqekmfehvd qlmvptlgsq egafenvrmn fsgeqgqtir qllsahmirr vamcclaspq
     2041 gkrqhlavsh ekgkitllql sallkqadss krkltitrla sapvpftvls itgnpcnedf
     2101 lavcglkdch vltfsssgsv sehlvlhpql etgnyiikam wlpgsqtela lvtadfvkvy
     2161 dlsvdalnaq fffllptgrl rdccfvcpee gprhlllmss agyvycqpli eessarhgpf
     2221 yvtsvldiqh pdikdsvgtv agggvsiyys hvlqllflsy angksfmacm pqitseitdp
     2281 vliqlkpsag nnggsggttt tsagtnaatg attttvttas ntsggsgsas skptpqplcq
     2341 wsevpnhpgl ilalmqfsnn plvlmvkpgt vlvqeikilt akskitdlva irhptssgem
     2401 rttlillced gslriymasq eqtgywltpa fqptaivglt gvtpaglssg atavgsatpr
     2461 psrrkkslrg ragpstlpms fpvdffehct piqdvefggn dllqiynaqq vkhrlnttgm
     2521 yvactkpggl tleitntdtt mvmvgvrvlv gsqdvqraps tvelfgrshp liltrsrwyd
     2581 lpfsreesls adkkinlffg pssdpscvtm vdsvkvygks kesfgwpees defatgvgia
     2641 aqsntvaatg idpdgipsap lpltaldrlt ssclemlddc fslsslseew lsggakgsal
     2701 dvatrlltlp tppavhqqvr allsalhpsr taytshrdqa llsyvlecln nsskekppfq
     2761 dldgeafyrl vaiargvaca rpanlvqfae sheaatgpae gvdemnkeev leerglplkv
     2821 hiatntlpps esshflsila eafwhlhrqr pqnpalapis khglvhieat vqalvevmya
     2881 fvaldmenvg lvaklfarll lcedtvvsfa akqaiirvlr pktrkrkvfi prtalcstpd
     2941 ggkastsgat gvsqslstts qplvsmpgss sstlagfdvq aqaslesqpd veshfemles
     3001 mdavvllgga ggagaggafp plldippdad detmvelaia lslqdqpgqs edlhlgalgs
     3061 agsqgpsqra sslegghysd ttasagasdd egstaatdgs tlrtspaeqq ggsgagsesg
     3121 gsgadsmtge pnvsgrssay gdnalpeggl arsetssvgl pptlpqedpd tawglhalrc
     3181 alldrllrfl pqlrsvggvr aipfmqvvlm lssdldsede rdratldnll scligeldrn
     3241 varvversps seeqlivmrl lsimmsriks sakvsgpsds acqncstsaa valhasgivd
     3301 hclqvlgtll ehwksvqntd sdtpggsass ttgppllkph paspppdmsp fflrqyvkgh
     3361 andvfeaypq lltemalrlp yqvkkvwsvv psgcvaptfg stwysylcey mmvqqtpfir
     3421 rqvrklllfl cgskekyrql rdlhaleshl agartvctsp pmgatplpyd alilivehlk
     3481 acaevatsrt anwqrycqhd ntvlpflfqv sflldegvas iilqllqcal cgtallqqpa
     3541 qpqqgasklh atsgspfkqp hrrerdtsee pdevegsdea qcaalaqqvh nsversllgq
     3601 fvrsfllecn stavrwqahs lihqlhrhsq pshrealltl mwelwphlps ygrkaaqfvd
     3661 llgyftlkap qpqwefteka lavlhdqnql lsnhpnssly nsirglvemd gyylesepcl
     3721 vcnnpevaya niklsaikvd srfttstqiv klvgshtvsr ivlrigdlkr skmvrainiy
     3781 ynnrsvqsvv elknrpamwh rakrcslaag qtelkmdfpl pivacnlmie yadfyenvqa
     3841 ssetlqcprc sasvpatpgv cancgenvfq chkcrainyd ekdpflcnsc gfckyakfdy
     3901 tltakpccav dpiegeedrk kavasinsll ekadkvyrql mahkpalell llkvqeqslv
     3961 draleeeaaa aaitatgaat tgatsatpan tasasgatcs mvnrsiqqla qkycseckas
     4021 fdelskivqk ilasrkelve yehkqkdsrk aqaatptvaa vrpgrgelps pgnfsapagn
     4081 kqssgrcygc asaavdhcit llralatnvp yrqllcergl ivelvehnlr qgwsqvgqlv
     4141 clltrdnpqa tdhlnsllmg ritlallrgg qpseqpclav vpatrpttad dteaaatppv
     4201 vvgvpdlpaa vrhevallal slqkedscwe qrlrcvmrlf lmgissqspi vlesitlpcl
     4261 kvlqgiikpd ppiskrnkdk sieelatvrp sgmcvavdlk gwldgdpvqt fsawksrclg
     4321 rpadtlslgg gkrqrredvr arflvekygl rwrqlarrgg ssmrllmllq ppqqqpaqgl
     4381 pcqprplqsn wlrqilfnps crqartvacs lvkalcqvps rcremldllt tylddlgtag
     4441 esgakflaly qsliapdhwk hylaikgllp rlgnlitaei eqltlleett lnadlsqgfa
     4501 lkmltellas fievdsirqq ykarlvgcvl ngylslrklv vqrtklvdet qekllallee
     4561 mttgtesete afmavcvetl grygledlrt pvfiferlcs lihpeenelg eflvtlekdp
     4621 qqedflqgrm lgnpysshep gmgplmrdlk nkvcqdcelv alleddngme llvcnkimsl
     4681 dlpvkevyrk vwcpengese amrvvyrmrg llgdateefv esleakaggp qvdeeqlyql
     4741 arvmgpcggl eamlerldai ddlsrgrpll tvllklfglc vkvrsnrlhl lepslravac
     4801 llgalrlllg aqeaalaeql latleavlae gaahvppqlp egvtkdditf lldqvgtgrp
     4861 sprllqllmr vvpfltltde akmdvlinhf rrqlnfirfd lehtpeddvq lecfcnlsag
     4921 ierndngnrl kdllitrgiv egaiqyllvy appaknslls asenwkefts kpalkyvlri
     4981 ltglsrghek tqilvsteci piihrleqvs sdehvgslae nlmealkenp nvaekieevr
     5041 kqtrdekkrl amamrekqlg elgmhtnekg qvtakssilk qmedlgeeag lvciicregy
     5101 kfqpgkllgi ytftkrcnld dfeakprktp gystvthfni vhvdchmaav rhargrdewe
     5161 saalqnantk cngllplwgp qvpesqfasc larhntyiqd ctghldvgym stlhdlklll
     5221 srfafersfs edsggggpqs nmhlipyllh mvlyvinttr cvareeknls nflemsperq
     5281 vencfesegp cywatmalav wshnrwqygr aslvrrmlil aharhlspqg cstlpdmvpr
     5341 efavyrpylc flgmvdglyn imfkkvacst ddgwsvalad yirhndqlhl elgdkllrtf
     5401 eeqvltcqsf refcdymgpm weidnpdafl healqlrv
//
LOCUS       XP_077505274            5403 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase-like protein poe isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505274
VERSION     XP_077505274.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649148.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..5403
                     /product="E3 ubiquitin-protein ligase-like protein poe
                     isoform X3"
                     /calculated_mol_wt=590894
     Region          54..1724
                     /region_name="E3_UBR4_N"
                     /note="E3 ubiquitin-protein ligase UBR4 N-terminal;
                     pfam19423"
                     /db_xref="CDD:466074"
     Region          1734..1804
                     /region_name="UBR-box_UBR4"
                     /note="UBR-box found in RING-type E3 ubiquitin-protein
                     ligase UBR4 and similar proteins; cd19680"
                     /db_xref="CDD:439078"
     Site            order(1753,1756,1765,1768,1772..1773,1776,1788,1790,1798)
                     /site_type="other"
                     /note="putative Zn binding site [ion binding]"
                     /db_xref="CDD:439078"
     Region          3812..3856
                     /region_name="DZR"
                     /note="Double zinc ribbon; pfam12773"
                     /db_xref="CDD:432773"
     Region          4595..5383
                     /region_name="E3_UbLigase_R4"
                     /note="E3 ubiquitin-protein ligase UBR4; pfam13764"
                     /db_xref="CDD:433459"
     CDS             1..5403
                     /gene="poe"
                     /coded_by="XM_077649148.1:51..16262"
                     /db_xref="GeneID:144115023"
ORIGIN      
        1 masassgpdw fsnavtilts nltslnksdl qiiiksviqs esvllaeeed crefreafva
       61 lsahfinasa tslsrsqivt aaqacsillk vllkclkahn ansllqqnli mvligglcis
      121 nagtpiksel stlssalksa qlpsslaskp dgaverrrdd trelrhlwln pstcileqls
      181 tpvldnamgp ksdvsadldk dldlsashii dpgmeaknfl laknvaslqr eragdilldv
      241 clalpsvvqy sqgledvlls cgtmaldcqt snmvltgska lfadillvck clglpvlepl
      301 gearlkrlvr lalgcghmav avtqavcvlg vsstgggsqp asgatkgvpc wddeghmala
      361 ahvveacldi ynviastmrs ssragghvlq nlhvltswll lrglqailgl ntfldraack
      421 egagtkgnrg gldqtptrtr dqsaglkqnl lriqqgfgvl pvalanqvls lltsifgelr
      481 leagaaleea drgagpaeer ivifldnint pfsawkcvrv lmqlnlpall fdlvsvsyrk
      541 agmlrriqkh ptdgdnfsts dsntfyeedf stsddssade aagpktsgql gessghprts
      601 lhkkrarssn lqtggrshpr ktnagqvfqe vrdddsepil ghwyeqtiap rehspgpgal
      661 qpkgapetev penrgvrcsl adgrplipdk eephgyiqls skvinflnay lvnfepvqgy
      721 lksgigtpqm vilagiikdl dretckssig llfgaslgqm yeefskalar fihnilatgl
      781 lddvyqdgll thlninpwtq kewplqiywr tlsvlaqvll lrlqkdkddl rsendtacil
      841 ifrlvlntmq kailsshvdd nedlnvehtq lllflfhnlq lmqkkvvlls vaktiinvap
      901 vsqsplkdvq iiyiarllhi feyliknlyd apaalvdqvq anlftlhgsa cispdtetak
      961 qhgklffpck eveenfaknt lsdvvqgahi rpkfyhlmil esgtqdvpkl dgfacsflla
     1021 spdvlnysdl ygavlsllwi gaqcdavkrp sdypklsylg aaavqycfga vwrlllslpp
     1081 siqlsealat gnamldlpqi lhaivwvsrf ghknfniwik dslvkqgmta qraestlhqv
     1141 tanasqllfd vrvfkqlask qlgqgpadls gpvsrselph lmdlfvleal vaklqvsldv
     1201 tllkpsnesh srevkefswe ifpillrlik lyntcirwsl llqavelspt grsetslram
     1261 dvvlrmtsrr csqvsstvaa ivshlppqvd stldfwnama vnvcnwrndf andslpsesy
     1321 iaavqvahft plsanstfsi nsslkrilqc ivrfaqdvys wsaedkfsnd llramlplll
     1381 dattesqadf vmlalegiig ssdteefaqh vyeemlancy ellinyshke seinelvfhe
     1441 clkfmeslld kqpgkkalek ffcdkdiwni llsaaegnls paygtrvlkf fsklftqidk
     1501 nvgdpslekl natlckmnrl neldpkslqn wltkvvvgsk aancsedgsp vqenrnllqn
     1561 lttyivkenn kvstqvagsf ltalieigtq llspasegvg fsdlmvvmst lasagsgsgh
     1621 vqlfmaatdw letckkylsq kdvleklean vssgkhqvim nsacyllsyv adlvgslkll
     1681 aerplivgrl gaasppldgd aqhpepdsdw adelgpedde sggedsdeds lcnklctftm
     1741 tqkefmnqhw yhchtcrmvd gvgvcticak vchkdhdvty akfgsffcdc gakedgscqa
     1801 lvkrnpqmls dtgcgaastt aafslasenv lpssirrras sphqgggtga ntadgrppaa
     1861 sadskkacde tlkhrqqlak kleafrgvli ehivstnlva tvleliqflm paivetynqn
     1921 spigsstraq kalaelhtqe kmfehvdqlm vptlgsqega fenvrmnfsg eqgqtirqll
     1981 sahmirrvam cclaspqgkr qhlavshekg kitllqlsal lkqadsskrk ltitrlasap
     2041 vpftvlsitg npcnedflav cglkdchvlt fsssgsvseh lvlhpqletg nyiikamwlp
     2101 gsqtelalvt adfvkvydls vdalnaqfff llptgrlrdc cfvcpeegpr hlllmssagy
     2161 vycqpliees sarhgpfyvt svldiqhpdi kdsvgtvagg gvsiyyshvl qllflsyang
     2221 ksfmacmpqi tseitdpvli qlkpsagnng gsggttttsa gtnaatgatt ttvttasnts
     2281 ggsgsasskp tpqplcqwse vpnhpglila lmqfsnnplv lmvkpgtvlv qeikiltaks
     2341 kitdlvairh ptssgemrtt lillcedgsl riymasqeqt gywltpafqp taivgltgvt
     2401 paglssgata vgsatprpsr rkkslrgrag pstlpmsfpv dffehctpiq dvefggndll
     2461 qiynaqqvkh rlnttgmyva ctkpggltle itntdttmvm vgvrvlvgsq dvqrapstve
     2521 lfgrshplil trsrwydlpf sreeslsadk kinlffgpss dpscvtmvds vkvygkskes
     2581 fgwpeesdef atgvgiaaqs ntvaatgidp dgipsaplpl taldrltssc lemlddcfsl
     2641 sslseewlsg gakgsaldva trlltlptpp avhqqvrall salhpsrtay tshrdqalls
     2701 yvleclnnss kekppfqdld geafyrlvai argvacarpa nlvqfaeshe aatgpaegvd
     2761 emnkeevlee rglplkvhia tntlppsess hflsilaeaf whlhrqrpqn palapiskhg
     2821 lvhieatvqa lvevmyafva ldmenvglva klfarlllce dtvvsfaakq aiirvlrpkt
     2881 rkrkvfiprt alcstpgevg tdhadggkas tsgatgvsqs lsttsqplvs mpgsssstla
     2941 gfdvqaqasl esqpdveshf emlesmdavv llggaggaga ggafpplldi ppdaddetmv
     3001 elaialslqd qpgqsedlhl galgsagsqg psqrassleg ghysdttasa gasddegsta
     3061 atdgstlrts paeqqggsga gsesggsgad smtgepnvsg rssaygdnal pegglarset
     3121 ssvglpptlp qedpdtawgl halrcalldr llrflpqlrs vggvraipfm qvvlmlssdl
     3181 dsederdrat ldnllsclig eldrnvarvv erspsseeql ivmrllsimm srikssakvs
     3241 gpsdsacqnc stsaavalha sgivdhclqv lgtllehwks vqntdsdtpg gsassttgpp
     3301 llkphpaspp pdmspfflrq yvkghandvf eaypqlltem alrlpyqvkk vwsvvpsgcv
     3361 aptfgstwys ylceymmvqq tpfirrqvrk lllflcgske kyrqlrdlha leshlagart
     3421 vctsppmgat plpydalili vehlkacaev atsrtanwqr ycqhdntvlp flfqvsflld
     3481 egvasiilql lqcalcgtal lqqpaqpqqg asklhatsgs pfkqphrrer dtseepdeve
     3541 gsdeaqcaal aqqvhnsver sllgqfvrsf llecnstavr wqahslihql hrhsqpshre
     3601 alltlmwelw phlpsygrka aqfvdllgyf tlkapqpqwe ftekalavlh dqnqllsnhp
     3661 nsslynsirg lvemdgyyle sepclvcnnp evayanikls aikvdsrftt stqivklvgs
     3721 htvsrivlri gdlkrskmvr ainiyynnrs vqsvvelknr pamwhrakrc slaagqtelk
     3781 mdfplpivac nlmieyadfy envqassetl qcprcsasvp atpgvcancg envfqchkcr
     3841 ainydekdpf lcnscgfcky akfdytltak pccavdpieg eedrkkavas insllekadk
     3901 vyrqlmahkp alellllkvq eqslvdrale eeaaaaaita tgaattgats atpantasas
     3961 gatcsmvnrs iqqlaqkycs eckasfdels kivqkilasr kelveyehkq kdsrkaqaat
     4021 ptvaavrpgr gelpspgnfs apagnkqssg rcygcasaav dhcitllral atnvpyrqll
     4081 cerglivelv ehnlrqgwsq vgqlvclltr dnpqatdhln sllmgritla llrggqpseq
     4141 pclavvpatr pttaddteaa atppvvvgvp dlpaavrhev allalslqke dscweqrlrc
     4201 vmrlflmgis sqspivlesi tlpclkvlqg iikpdppisk rnkdksieel atvrpsgmcv
     4261 avdlkgwldg dpvqtfsawk srclgrpadt lslgggkrqr redvrarflv ekyglrwrql
     4321 arrggssmrl lmllqppqqq paqglpcqpr plqsnwlrqi lfnpscrqar tvacslvkal
     4381 cqvpsrcrem ldllttyldd lgtagesgak flalyqslia pdhwkhylai kgllprlgnl
     4441 itaeieqltl leettlnadl sqgfalkmlt ellasfievd sirqqykarl vgcvlngyls
     4501 lrklvvqrtk lvdetqekll alleemttgt eseteafmav cvetlgrygl edlrtpvfif
     4561 erlcslihpe enelgeflvt lekdpqqedf lqgrmlgnpy sshepgmgpl mrdlknkvcq
     4621 dcelvalled dngmellvcn kimsldlpvk evyrkvwcpe ngeseamrvv yrmrgllgda
     4681 teefveslea kaggpqvdee qlyqlarvmg pcggleamle rldaiddlsr grplltvllk
     4741 lfglcvkvrs nrlhllepsl ravacllgal rlllgaqeaa laeqllatle avlaegaahv
     4801 ppqlpegvtk dditflldqv gtgrpsprll qllmrvvpfl tltdeakmdv linhfrrqln
     4861 firfdlehtp eddvqlecfc nlsagiernd ngnrlkdlli trgivegaiq yllvyappak
     4921 nsllsasenw keftskpalk yvlriltgls rghektqilv stecipiihr leqvssdehv
     4981 gslaenlmea lkenpnvaek ieevrkqtrd ekkrlamamr ekqlgelgmh tnekgqvtak
     5041 ssilkqmedl geeaglvcii cregykfqpg kllgiytftk rcnlddfeak prktpgystv
     5101 thfnivhvdc hmaavrharg rdewesaalq nantkcngll plwgpqvpes qfasclarhn
     5161 tyiqdctghl dvgymstlhd lklllsrfaf ersfsedsgg ggpqsnmhli pyllhmvlyv
     5221 inttrcvare eknlsnflem sperqvencf esegpcywat malavwshnr wqygraslvr
     5281 rmlilaharh lspqgcstlp dmvprefavy rpylcflgmv dglynimfkk vacstddgws
     5341 valadyirhn dqlhlelgdk llrtfeeqvl tcqsfrefcd ymgpmweidn pdaflhealq
     5401 lrv
//
LOCUS       XP_077505275            5394 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase-like protein poe isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505275
VERSION     XP_077505275.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649149.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..5394
                     /product="E3 ubiquitin-protein ligase-like protein poe
                     isoform X4"
                     /calculated_mol_wt=590380
     Region          54..1715
                     /region_name="E3_UBR4_N"
                     /note="E3 ubiquitin-protein ligase UBR4 N-terminal;
                     pfam19423"
                     /db_xref="CDD:466074"
     Region          1725..1795
                     /region_name="UBR-box_UBR4"
                     /note="UBR-box found in RING-type E3 ubiquitin-protein
                     ligase UBR4 and similar proteins; cd19680"
                     /db_xref="CDD:439078"
     Site            order(1744,1747,1756,1759,1763..1764,1767,1779,1781,1789)
                     /site_type="other"
                     /note="putative Zn binding site [ion binding]"
                     /db_xref="CDD:439078"
     Region          3803..3847
                     /region_name="DZR"
                     /note="Double zinc ribbon; pfam12773"
                     /db_xref="CDD:432773"
     Region          4586..5374
                     /region_name="E3_UbLigase_R4"
                     /note="E3 ubiquitin-protein ligase UBR4; pfam13764"
                     /db_xref="CDD:433459"
     CDS             1..5394
                     /gene="poe"
                     /coded_by="XM_077649149.1:51..16235"
                     /db_xref="GeneID:144115023"
ORIGIN      
        1 masassgpdw fsnavtilts nltslnksdl qiiiksviqs esvllaeeed crefreafva
       61 lsahfinasa tslsrsqivt aaqacsillk vllkclkahn ansllqqnli mvligglcis
      121 nagtpiksel stlssalksa qlpsslaskp dgaverrrdd trelrhlwln pstcileqls
      181 tpvldnamgp ksdvsadldk dldlsashii dpgmeaknfl laknvaslqr eragdilldv
      241 clalpsvvqy sqgledvlls cgtmaldcqt snmvltgska lfadillvck clglpvlepl
      301 gearlkrlvr lalgcghmav avtqavcvlg vsstgggsqp asgatkgvpc wddeghmala
      361 ahvveacldi ynviastmrs ssragghvlq nlhvltswll lrglqailgl ntfldraack
      421 egagtkgnrg gldqtptrtr dqsaglkqnl lriqqgfgvl pvalanqvls lltsifgelr
      481 leagaaleea drgagpaeer ivifldnint pfsawkcvrv lmqlnlpall fdlvsvsyrk
      541 agmlrriqkh ptdgdnfsts dsntfyeedf stsddssade dddsepilgh wyeqtiapre
      601 hspgpgalqp kgapetevpe nrgvrcslad grplipdkee phgyiqlssk vinflnaylv
      661 nfepvqgylk sgigtpqmvi lagiikdldr etckssigll fgaslgqmye efskalarfi
      721 hnilatglld dvyqdgllth lninpwtqke wplqiywrtl svlaqvlllr lqkdkddlrs
      781 endtacilif rlvlntmqka ilsshvddne dlnvehtqll lflfhnlqlm qkkvvllsva
      841 ktiinvapvs qsplkdvqii yiarllhife yliknlydap aalvdqvqan lftlhgsaci
      901 spdtetakqh gklffpckev eenfakntls dvvqgahirp kfyhlmiles gtqdvpkldg
      961 facsfllasp dvlnysdlyg avlsllwiga qcdavkrpsd ypklsylgaa avqycfgavw
     1021 rlllslppsi qlsealatgn amldlpqilh aivwvsrfgh knfniwikvs safcmlpctf
     1081 rsrpillhdl hlrrrsfpsl klllrwklsa adslvkqgmt aqraestlhq vtanasqllf
     1141 dvrvfkqlas kqlgqgpadl sgpvsrselp hlmdlfvlea lvaklqvsld vtllkpsnes
     1201 hsrevkefsw eifpillrli klyntcirws lllqavelsp tgrsetslra mdvvlrmtsr
     1261 rcsqvsstva aivshlppqv dstldfwnam avnvcnwrnd fandslpses yiaavqvahf
     1321 tplsanstfs insslkrilq civrfaqdvy swsaedkfsn dllramlpll ldattesqad
     1381 fvmlalegii gssdteefaq hvyeemlanc yellinyshk eseinelvfh eclkfmesll
     1441 dkqpgkkale kffcdkdiwn illsaaegnl spaygtrvlk ffsklftqid knvgdpslek
     1501 lnatlckmnr lneldpkslq nwltkvvvgs kaancsedgs pvqenrnllq nlttyivken
     1561 nkvstqvags fltalieigt qllspasegv gfsdlmvvms tlasagsgsg hvqlfmaatd
     1621 wletckkyls qkdvleklea nvssgkhqvi mnsacyllsy vadlvgslkl laerplivgr
     1681 lgaasppldg daqhpepdsd wadelgpedd esggedsded slcnklctft mtqkefmnqh
     1741 wyhchtcrmv dgvgvctica kvchkdhdvt yakfgsffcd cgakedgscq alvkrnpqml
     1801 sdtgcgaast taafslasen vlpssirrra ssphqgggtg antadgrppa asadskkacd
     1861 etlkhrqqla kkleafrgvl iehivstnlv atvleliqfl mpaivetynq nspigsstra
     1921 qkalaelhtq ekmfehvdql mvptlgsqeg afenvrmnfs geqgqtirql lsahmirrva
     1981 mcclaspqgk rqhlavshek gkitllqlsa llkqadsskr kltitrlasa pvpftvlsit
     2041 gnpcnedfla vcglkdchvl tfsssgsvse hlvlhpqlet gnyiikamwl pgsqtelalv
     2101 tadfvkvydl svdalnaqff fllptgrlrd ccfvcpeegp rhlllmssag yvycqpliee
     2161 ssarhgpfyv tsvldiqhpd ikdsvgtvag ggvsiyyshv lqllflsyan gksfmacmpq
     2221 itseitdpvl iqlkpsagnn ggsggtttts agtnaatgat tttvttasnt sggsgsassk
     2281 ptpqplcqws evpnhpglil almqfsnnpl vlmvkpgtvl vqeikiltak skitdlvair
     2341 hptssgemrt tlillcedgs lriymasqeq tgywltpafq ptaivgltgv tpaglssgat
     2401 avgsatprps rrkkslrgra gpstlpmsfp vdffehctpi qdvefggndl lqiynaqqvk
     2461 hrlnttgmyv actkpggltl eitntdttmv mvgvrvlvgs qdvqrapstv elfgrshpli
     2521 ltrsrwydlp fsreeslsad kkinlffgps sdpscvtmvd svkvygkske sfgwpeesde
     2581 fatgvgiaaq sntvaatgid pdgipsaplp ltaldrltss clemlddcfs lsslseewls
     2641 ggakgsaldv atrlltlptp pavhqqvral lsalhpsrta ytshrdqall syvleclnns
     2701 skekppfqdl dgeafyrlva iargvacarp anlvqfaesh eaatgpaegv demnkeevle
     2761 erglplkvhi atntlppses shflsilaea fwhlhrqrpq npalapiskh glvhieatvq
     2821 alvevmyafv aldmenvglv aklfarlllc edtvvsfaak qaiirvlrpk trkrkvfipr
     2881 talcstpgev gtdhadggka stsgatgvsq slsttsqplv smpgsssstl agfdvqaqas
     2941 lesqpdvesh femlesmdav vllggaggag aggafpplld ippdaddetm velaialslq
     3001 dqpgqsedlh lgalgsagsq gpsqrassle gghysdttas agasddegst aatdgstlrt
     3061 spaeqqggsg agsesggsga dsmtgepnvs grssaygdna lpegglarse tssvglpptl
     3121 pqedpdtawg lhalrcalld rllrflpqlr svggvraipf mqvvlmlssd ldsederdra
     3181 tldnllscli geldrnvarv verspsseeq livmrllsim msrikssakv sgpsdsacqn
     3241 cstsaavalh asgivdhclq vlgtllehwk svqntdsdtp ggsassttgp pllkphpasp
     3301 ppdmspfflr qyvkghandv feaypqllte malrlpyqvk kvwsvvpsgc vaptfgstwy
     3361 sylceymmvq qtpfirrqvr klllflcgsk ekyrqlrdlh aleshlagar tvctsppmga
     3421 tplpydalil ivehlkacae vatsrtanwq rycqhdntvl pflfqvsfll degvasiilq
     3481 llqcalcgta llqqpaqpqq gasklhatsg spfkqphrre rdtseepdev egsdeaqcaa
     3541 laqqvhnsve rsllgqfvrs fllecnstav rwqahslihq lhrhsqpshr ealltlmwel
     3601 wphlpsygrk aaqfvdllgy ftlkapqpqw eftekalavl hdqnqllsnh pnsslynsir
     3661 glvemdgyyl esepclvcnn pevayanikl saikvdsrft tstqivklvg shtvsrivlr
     3721 igdlkrskmv rainiyynnr svqsvvelkn rpamwhrakr cslaagqtel kmdfplpiva
     3781 cnlmieyadf yenvqasset lqcprcsasv patpgvcanc genvfqchkc rainydekdp
     3841 flcnscgfck yakfdytlta kpccavdpie geedrkkava sinsllekad kvyrqlmahk
     3901 palellllkv qeqslvdral eeeaaaaait atgaattgat satpantasa sgatcsmvnr
     3961 siqqlaqkyc seckasfdel skivqkilas rkelveyehk qkdsrkaqaa tptvaavrpg
     4021 rgelpspgnf sapagnkqss grcygcasaa vdhcitllra latnvpyrql lcerglivel
     4081 vehnlrqgws qvgqlvcllt rdnpqatdhl nsllmgritl allrggqpse qpclavvpat
     4141 rpttaddtea aatppvvvgv pdlpaavrhe vallalslqk edscweqrlr cvmrlflmgi
     4201 ssqspivles itlpclkvlq giikpdppis krnkdksiee latvrpsgmc vavdlkgwld
     4261 gdpvqtfsaw ksrclgrpad tlslgggkrq rredvrarfl vekyglrwrq larrggssmr
     4321 llmllqppqq qpaqglpcqp rplqsnwlrq ilfnpscrqa rtvacslvka lcqvpsrcre
     4381 mldllttyld dlgtagesga kflalyqsli apdhwkhyla ikgllprlgn litaeieqlt
     4441 lleettlnad lsqgfalkml tellasfiev dsirqqykar lvgcvlngyl slrklvvqrt
     4501 klvdetqekl lalleemttg teseteafma vcvetlgryg ledlrtpvfi ferlcslihp
     4561 eenelgeflv tlekdpqqed flqgrmlgnp ysshepgmgp lmrdlknkvc qdcelvalle
     4621 ddngmellvc nkimsldlpv kevyrkvwcp engeseamrv vyrmrgllgd ateefvesle
     4681 akaggpqvde eqlyqlarvm gpcggleaml erldaiddls rgrplltvll klfglcvkvr
     4741 snrlhlleps lravacllga lrlllgaqea alaeqllatl eavlaegaah vppqlpegvt
     4801 kdditflldq vgtgrpsprl lqllmrvvpf ltltdeakmd vlinhfrrql nfirfdleht
     4861 peddvqlecf cnlsagiern dngnrlkdll itrgivegai qyllvyappa knsllsasen
     4921 wkeftskpal kyvlriltgl srghektqil vstecipiih rleqvssdeh vgslaenlme
     4981 alkenpnvae kieevrkqtr dekkrlamam rekqlgelgm htnekgqvta kssilkqmed
     5041 lgeeaglvci icregykfqp gkllgiytft krcnlddfea kprktpgyst vthfnivhvd
     5101 chmaavrhar grdewesaal qnantkcngl lplwgpqvpe sqfasclarh ntyiqdctgh
     5161 ldvgymstlh dlklllsrfa fersfsedsg gggpqsnmhl ipyllhmvly vinttrcvar
     5221 eeknlsnfle msperqvenc fesegpcywa tmalavwshn rwqygraslv rrmlilahar
     5281 hlspqgcstl pdmvprefav yrpylcflgm vdglynimfk kvacstddgw svaladyirh
     5341 ndqlhlelgd kllrtfeeqv ltcqsfrefc dymgpmweid npdaflheal qlrv
//
LOCUS       XP_077505276            5351 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase-like protein poe isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077505276
VERSION     XP_077505276.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649150.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5351
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..5351
                     /product="E3 ubiquitin-protein ligase-like protein poe
                     isoform X5"
                     /calculated_mol_wt=585360
     Region          54..1672
                     /region_name="E3_UBR4_N"
                     /note="E3 ubiquitin-protein ligase UBR4 N-terminal;
                     pfam19423"
                     /db_xref="CDD:466074"
     Region          1682..1752
                     /region_name="UBR-box_UBR4"
                     /note="UBR-box found in RING-type E3 ubiquitin-protein
                     ligase UBR4 and similar proteins; cd19680"
                     /db_xref="CDD:439078"
     Site            order(1701,1704,1713,1716,1720..1721,1724,1736,1738,1746)
                     /site_type="other"
                     /note="putative Zn binding site [ion binding]"
                     /db_xref="CDD:439078"
     Region          3760..3804
                     /region_name="DZR"
                     /note="Double zinc ribbon; pfam12773"
                     /db_xref="CDD:432773"
     Region          4543..5331
                     /region_name="E3_UbLigase_R4"
                     /note="E3 ubiquitin-protein ligase UBR4; pfam13764"
                     /db_xref="CDD:433459"
     CDS             1..5351
                     /gene="poe"
                     /coded_by="XM_077649150.1:54..16109"
                     /db_xref="GeneID:144115023"
ORIGIN      
        1 masassgpdw fsnavtilts nltslnksdl qiiiksviqs esvllaeeed crefreafva
       61 lsahfinasa tslsrsqivt aaqacsillk vllkclkahn ansllqqnli mvligglcis
      121 nagtpiksel stlssalksa qlpsslaskp dgaverrrdd trelrhlwln pstcileqls
      181 tpvldnamgp ksdvsadldk dldlsashii dpgmeaknfl laknvaslqr eragdilldv
      241 clalpsvvqy sqgledvlls cgtmaldcqt snmvltgska lfadillvck clglpvlepl
      301 gearlkrlvr lalgcghmav avtqavcvlg vsstgggsqp asgatkgvpc wddeghmala
      361 ahvveacldi ynviastmrs ssragghvlq nlhvltswll lrglqailgl ntfldraack
      421 egagtkgnrg gldqtptrtr dqsaglkqnl lriqqgfgvl pvalanqvls lltsifgelr
      481 leagaaleea drgagpaeer ivifldnint pfsawkcvrv lmqlnlpall fdlvsvsyrk
      541 agmlrriqkh ptdgdnfsts dsntfyeedf stsddssade dddsepilgh wyeqtiapre
      601 hspgpgalqp kgapetevpe nrgvrcslad grplipdkee phgyiqlssk vinflnaylv
      661 nfepvqgylk sgigtpqmvi lagiikdldr etckssigll fgaslgqmye efskalarfi
      721 hnilatglld dvyqdgllth lninpwtqke wplqiywrtl svlaqvlllr lqkdkddlrs
      781 endtacilif rlvlntmqka ilsshvddne dlnvehtqll lflfhnlqlm qkkvvllsva
      841 ktiinvapvs qsplkdvqii yiarllhife yliknlydap aalvdqvqan lftlhgsaci
      901 spdtetakqh gklffpckev eenfakntls dvvqgahirp kfyhlmiles gtqdvpkldg
      961 facsfllasp dvlnysdlyg avlsllwiga qcdavkrpsd ypklsylgaa avqycfgavw
     1021 rlllslppsi qlsealatgn amldlpqilh aivwvsrfgh knfniwikds lvkqgmtaqr
     1081 aestlhqvta nasqllfdvr vfkqlaskql gqgpadlsgp vsrselphlm dlfvlealva
     1141 klqvsldvtl lkpsneshsr evkefsweif pillrlikly ntcirwslll qavelsptgr
     1201 setslramdv vlrmtsrrcs qvsstvaaiv shlppqvdst ldfwnamavn vcnwrndfan
     1261 dslpsesyia avqvahftpl sanstfsins slkrilqciv rfaqdvysws aedkfsndll
     1321 ramlplllda ttesqadfvm lalegiigss dteefaqhvy eemlancyel linyshkese
     1381 inelvfhecl kfmeslldkq pgkkalekff cdkdiwnill saaegnlspa ygtrvlkffs
     1441 klftqidknv gdpsleklna tlckmnrlne ldpkslqnwl tkvvvgskaa ncsedgspvq
     1501 enrnllqnlt tyivkennkv stqvagsflt alieigtqll spasegvgfs dlmvvmstla
     1561 sagsgsghvq lfmaatdwle tckkylsqkd vlekleanvs sgkhqvimns acyllsyvad
     1621 lvgslkllae rplivgrlga asppldgdaq hpepdsdwad elgpeddesg gedsdedslc
     1681 nklctftmtq kefmnqhwyh chtcrmvdgv gvcticakvc hkdhdvtyak fgsffcdcga
     1741 kedgscqalv krnpqmlsdt gcgaasttaa fslasenvlp ssirrrassp hqgggtgant
     1801 adgrppaasa dskkacdetl khrqqlakkl eafrgvlieh ivstnlvatv leliqflmpa
     1861 ivetynqnsp igsstraqka laelhtqekm fehvdqlmvp tlgsqegafe nvrmnfsgeq
     1921 gqtirqllsa hmirrvamcc laspqgkrqh lavshekgki tllqlsallk qadsskrklt
     1981 itrlasapvp ftvlsitgnp cnedflavcg lkdchvltfs ssgsvsehlv lhpqletgny
     2041 iikamwlpgs qtelalvtad fvkvydlsvd alnaqfffll ptgrlrdccf vcpeegprhl
     2101 llmssagyvy cqplieessa rhgpfyvtsv ldiqhpdikd svgtvagggv siyyshvlql
     2161 lflsyangks fmacmpqits eitdpvliql kpsagnnggs ggttttsagt naatgatttt
     2221 vttasntsgg sgsasskptp qplcqwsevp nhpglilalm qfsnnplvlm vkpgtvlvqe
     2281 ikiltakski tdlvairhpt ssgemrttli llcedgslri ymasqeqtgy wltpafqpta
     2341 ivgltgvtpa glssgatavg satprpsrrk kslrgragps tlpmsfpvdf fehctpiqdv
     2401 efggndllqi ynaqqvkhrl nttgmyvact kpggltleit ntdttmvmvg vrvlvgsqdv
     2461 qrapstvelf grshpliltr srwydlpfsr eeslsadkki nlffgpssdp scvtmvdsvk
     2521 vygkskesfg wpeesdefat gvgiaaqsnt vaatgidpdg ipsaplplta ldrltsscle
     2581 mlddcfslss lseewlsgga kgsaldvatr lltlptppav hqqvrallsa lhpsrtayts
     2641 hrdqallsyv leclnnsske kppfqdldge afyrlvaiar gvacarpanl vqfaesheaa
     2701 tgpaegvdem nkeevleerg lplkvhiatn tlppsesshf lsilaeafwh lhrqrpqnpa
     2761 lapiskhglv hieatvqalv evmyafvald menvglvakl farlllcedt vvsfaakqai
     2821 irvlrpktrk rkvfiprtal cstpgevgtd hadggkasts gatgvsqsls ttsqplvsmp
     2881 gsssstlagf dvqaqasles qpdveshfem lesmdavvll ggaggagagg afpplldipp
     2941 daddetmvel aialslqdqp gqsedlhlga lgsagsqgps qrassleggh ysdttasaga
     3001 sddegstaat dgstlrtspa eqqggsgags esggsgadsm tgepnvsgrs saygdnalpe
     3061 gglarsetss vglpptlpqe dpdtawglha lrcalldrll rflpqlrsvg gvraipfmqv
     3121 vlmlssdlds ederdratld nllscligel drnvarvver spsseeqliv mrllsimmsr
     3181 ikssakvsgp sdsacqncst saavalhasg ivdhclqvlg tllehwksvq ntdsdtpggs
     3241 assttgppll kphpaspppd mspfflrqyv kghandvfea ypqlltemal rlpyqvkkvw
     3301 svvpsgcvap tfgstwysyl ceymmvqqtp firrqvrkll lflcgskeky rqlrdlhale
     3361 shlagartvc tsppmgatpl pydalilive hlkacaevat srtanwqryc qhdntvlpfl
     3421 fqvsflldeg vasiilqllq calcgtallq qpaqpqqgas klhatsgspf kqphrrerdt
     3481 seepdevegs deaqcaalaq qvhnsversl lgqfvrsfll ecnstavrwq ahslihqlhr
     3541 hsqpshreal ltlmwelwph lpsygrkaaq fvdllgyftl kapqpqweft ekalavlhdq
     3601 nqllsnhpns slynsirglv emdgyylese pclvcnnpev ayaniklsai kvdsrfttst
     3661 qivklvgsht vsrivlrigd lkrskmvrai niyynnrsvq svvelknrpa mwhrakrcsl
     3721 aagqtelkmd fplpivacnl mieyadfyen vqassetlqc prcsasvpat pgvcancgen
     3781 vfqchkcrai nydekdpflc nscgfckyak fdytltakpc cavdpiegee drkkavasin
     3841 sllekadkvy rqlmahkpal ellllkvqeq slvdraleee aaaaaitatg aattgatsat
     3901 pantasasga tcsmvnrsiq qlaqkycsec kasfdelski vqkilasrke lveyehkqkd
     3961 srkaqaatpt vaavrpgrge lpspgnfsap agnkqssgrc ygcasaavdh citllralat
     4021 nvpyrqllce rglivelveh nlrqgwsqvg qlvclltrdn pqatdhlnsl lmgritlall
     4081 rggqpseqpc lavvpatrpt taddteaaat ppvvvgvpdl paavrheval lalslqkeds
     4141 cweqrlrcvm rlflmgissq spivlesitl pclkvlqgii kpdppiskrn kdksieelat
     4201 vrpsgmcvav dlkgwldgdp vqtfsawksr clgrpadtls lgggkrqrre dvrarflvek
     4261 yglrwrqlar rggssmrllm llqppqqqpa qglpcqprpl qsnwlrqilf npscrqartv
     4321 acslvkalcq vpsrcremld llttylddlg tagesgakfl alyqsliapd hwkhylaikg
     4381 llprlgnlit aeieqltlle ettlnadlsq gfalkmltel lasfievdsi rqqykarlvg
     4441 cvlngylslr klvvqrtklv detqekllal leemttgtes eteafmavcv etlgrygled
     4501 lrtpvfifer lcslihpeen elgeflvtle kdpqqedflq grmlgnpyss hepgmgplmr
     4561 dlknkvcqdc elvalleddn gmellvcnki msldlpvkev yrkvwcpeng eseamrvvyr
     4621 mrgllgdate efvesleaka ggpqvdeeql yqlarvmgpc ggleamlerl daiddlsrgr
     4681 plltvllklf glcvkvrsnr lhllepslra vacllgalrl llgaqeaala eqllatleav
     4741 laegaahvpp qlpegvtkdd itflldqvgt grpsprllql lmrvvpfltl tdeakmdvli
     4801 nhfrrqlnfi rfdlehtped dvqlecfcnl sagierndng nrlkdllitr givegaiqyl
     4861 lvyappakns llsasenwke ftskpalkyv lriltglsrg hektqilvst ecipiihrle
     4921 qvssdehvgs laenlmealk enpnvaekie evrkqtrdek krlamamrek qlgelgmhtn
     4981 ekgqvtakss ilkqmedlge eaglvciicr egykfqpgkl lgiytftkrc nlddfeakpr
     5041 ktpgystvth fnivhvdchm aavrhargrd ewesaalqna ntkcngllpl wgpqvpesqf
     5101 asclarhnty iqdctghldv gymstlhdlk lllsrfafer sfsedsgggg pqsnmhlipy
     5161 llhmvlyvin ttrcvareek nlsnflemsp erqvencfes egpcywatma lavwshnrwq
     5221 ygraslvrrm lilaharhls pqgcstlpdm vprefavyrp ylcflgmvdg lynimfkkva
     5281 cstddgwsva ladyirhndq lhlelgdkll rtfeeqvltc qsfrefcdym gpmweidnpd
     5341 aflhealqlr v
//
LOCUS       XP_077505277            5343 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase-like protein poe isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077505277
VERSION     XP_077505277.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649151.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..5343
                     /product="E3 ubiquitin-protein ligase-like protein poe
                     isoform X6"
                     /calculated_mol_wt=584593
     Region          54..1672
                     /region_name="E3_UBR4_N"
                     /note="E3 ubiquitin-protein ligase UBR4 N-terminal;
                     pfam19423"
                     /db_xref="CDD:466074"
     Region          1682..1752
                     /region_name="UBR-box_UBR4"
                     /note="UBR-box found in RING-type E3 ubiquitin-protein
                     ligase UBR4 and similar proteins; cd19680"
                     /db_xref="CDD:439078"
     Site            order(1701,1704,1713,1716,1720..1721,1724,1736,1738,1746)
                     /site_type="other"
                     /note="putative Zn binding site [ion binding]"
                     /db_xref="CDD:439078"
     Region          3752..3796
                     /region_name="DZR"
                     /note="Double zinc ribbon; pfam12773"
                     /db_xref="CDD:432773"
     Region          4535..5323
                     /region_name="E3_UbLigase_R4"
                     /note="E3 ubiquitin-protein ligase UBR4; pfam13764"
                     /db_xref="CDD:433459"
     CDS             1..5343
                     /gene="poe"
                     /coded_by="XM_077649151.1:54..16085"
                     /db_xref="GeneID:144115023"
ORIGIN      
        1 masassgpdw fsnavtilts nltslnksdl qiiiksviqs esvllaeeed crefreafva
       61 lsahfinasa tslsrsqivt aaqacsillk vllkclkahn ansllqqnli mvligglcis
      121 nagtpiksel stlssalksa qlpsslaskp dgaverrrdd trelrhlwln pstcileqls
      181 tpvldnamgp ksdvsadldk dldlsashii dpgmeaknfl laknvaslqr eragdilldv
      241 clalpsvvqy sqgledvlls cgtmaldcqt snmvltgska lfadillvck clglpvlepl
      301 gearlkrlvr lalgcghmav avtqavcvlg vsstgggsqp asgatkgvpc wddeghmala
      361 ahvveacldi ynviastmrs ssragghvlq nlhvltswll lrglqailgl ntfldraack
      421 egagtkgnrg gldqtptrtr dqsaglkqnl lriqqgfgvl pvalanqvls lltsifgelr
      481 leagaaleea drgagpaeer ivifldnint pfsawkcvrv lmqlnlpall fdlvsvsyrk
      541 agmlrriqkh ptdgdnfsts dsntfyeedf stsddssade dddsepilgh wyeqtiapre
      601 hspgpgalqp kgapetevpe nrgvrcslad grplipdkee phgyiqlssk vinflnaylv
      661 nfepvqgylk sgigtpqmvi lagiikdldr etckssigll fgaslgqmye efskalarfi
      721 hnilatglld dvyqdgllth lninpwtqke wplqiywrtl svlaqvlllr lqkdkddlrs
      781 endtacilif rlvlntmqka ilsshvddne dlnvehtqll lflfhnlqlm qkkvvllsva
      841 ktiinvapvs qsplkdvqii yiarllhife yliknlydap aalvdqvqan lftlhgsaci
      901 spdtetakqh gklffpckev eenfakntls dvvqgahirp kfyhlmiles gtqdvpkldg
      961 facsfllasp dvlnysdlyg avlsllwiga qcdavkrpsd ypklsylgaa avqycfgavw
     1021 rlllslppsi qlsealatgn amldlpqilh aivwvsrfgh knfniwikds lvkqgmtaqr
     1081 aestlhqvta nasqllfdvr vfkqlaskql gqgpadlsgp vsrselphlm dlfvlealva
     1141 klqvsldvtl lkpsneshsr evkefsweif pillrlikly ntcirwslll qavelsptgr
     1201 setslramdv vlrmtsrrcs qvsstvaaiv shlppqvdst ldfwnamavn vcnwrndfan
     1261 dslpsesyia avqvahftpl sanstfsins slkrilqciv rfaqdvysws aedkfsndll
     1321 ramlplllda ttesqadfvm lalegiigss dteefaqhvy eemlancyel linyshkese
     1381 inelvfhecl kfmeslldkq pgkkalekff cdkdiwnill saaegnlspa ygtrvlkffs
     1441 klftqidknv gdpsleklna tlckmnrlne ldpkslqnwl tkvvvgskaa ncsedgspvq
     1501 enrnllqnlt tyivkennkv stqvagsflt alieigtqll spasegvgfs dlmvvmstla
     1561 sagsgsghvq lfmaatdwle tckkylsqkd vlekleanvs sgkhqvimns acyllsyvad
     1621 lvgslkllae rplivgrlga asppldgdaq hpepdsdwad elgpeddesg gedsdedslc
     1681 nklctftmtq kefmnqhwyh chtcrmvdgv gvcticakvc hkdhdvtyak fgsffcdcga
     1741 kedgscqalv krnpqmlsdt gcgaasttaa fslasenvlp ssirrrassp hqgggtgant
     1801 adgrppaasa dskkacdetl khrqqlakkl eafrgvlieh ivstnlvatv leliqflmpa
     1861 ivetynqnsp igsstraqka laelhtqekm fehvdqlmvp tlgsqegafe nvrmnfsgeq
     1921 gqtirqllsa hmirrvamcc laspqgkrqh lavshekgki tllqlsallk qadsskrklt
     1981 itrlasapvp ftvlsitgnp cnedflavcg lkdchvltfs ssgsvsehlv lhpqletgny
     2041 iikamwlpgs qtelalvtad fvkvydlsvd alnaqfffll ptgrlrdccf vcpeegprhl
     2101 llmssagyvy cqplieessa rhgpfyvtsv ldiqhpdikd svgtvagggv siyyshvlql
     2161 lflsyangks fmacmpqits eitdpvliql kpsagnnggs ggttttsagt naatgatttt
     2221 vttasntsgg sgsasskptp qplcqwsevp nhpglilalm qfsnnplvlm vkpgtvlvqe
     2281 ikiltakski tdlvairhpt ssgemrttli llcedgslri ymasqeqtgy wltpafqpta
     2341 ivgltgvtpa glssgatavg satprpsrrk kslrgragps tlpmsfpvdf fehctpiqdv
     2401 efggndllqi ynaqqvkhrl nttgmyvact kpggltleit ntdttmvmvg vrvlvgsqdv
     2461 qrapstvelf grshpliltr srwydlpfsr eeslsadkki nlffgpssdp scvtmvdsvk
     2521 vygkskesfg wpeesdefat gvgiaaqsnt vaatgidpdg ipsaplplta ldrltsscle
     2581 mlddcfslss lseewlsgga kgsaldvatr lltlptppav hqqvrallsa lhpsrtayts
     2641 hrdqallsyv leclnnsske kppfqdldge afyrlvaiar gvacarpanl vqfaesheaa
     2701 tgpaegvdem nkeevleerg lplkvhiatn tlppsesshf lsilaeafwh lhrqrpqnpa
     2761 lapiskhglv hieatvqalv evmyafvald menvglvakl farlllcedt vvsfaakqai
     2821 irvlrpktrk rkvfiprtal cstpdggkas tsgatgvsqs lsttsqplvs mpgsssstla
     2881 gfdvqaqasl esqpdveshf emlesmdavv llggaggaga ggafpplldi ppdaddetmv
     2941 elaialslqd qpgqsedlhl galgsagsqg psqrassleg ghysdttasa gasddegsta
     3001 atdgstlrts paeqqggsga gsesggsgad smtgepnvsg rssaygdnal pegglarset
     3061 ssvglpptlp qedpdtawgl halrcalldr llrflpqlrs vggvraipfm qvvlmlssdl
     3121 dsederdrat ldnllsclig eldrnvarvv erspsseeql ivmrllsimm srikssakvs
     3181 gpsdsacqnc stsaavalha sgivdhclqv lgtllehwks vqntdsdtpg gsassttgpp
     3241 llkphpaspp pdmspfflrq yvkghandvf eaypqlltem alrlpyqvkk vwsvvpsgcv
     3301 aptfgstwys ylceymmvqq tpfirrqvrk lllflcgske kyrqlrdlha leshlagart
     3361 vctsppmgat plpydalili vehlkacaev atsrtanwqr ycqhdntvlp flfqvsflld
     3421 egvasiilql lqcalcgtal lqqpaqpqqg asklhatsgs pfkqphrrer dtseepdeve
     3481 gsdeaqcaal aqqvhnsver sllgqfvrsf llecnstavr wqahslihql hrhsqpshre
     3541 alltlmwelw phlpsygrka aqfvdllgyf tlkapqpqwe ftekalavlh dqnqllsnhp
     3601 nsslynsirg lvemdgyyle sepclvcnnp evayanikls aikvdsrftt stqivklvgs
     3661 htvsrivlri gdlkrskmvr ainiyynnrs vqsvvelknr pamwhrakrc slaagqtelk
     3721 mdfplpivac nlmieyadfy envqassetl qcprcsasvp atpgvcancg envfqchkcr
     3781 ainydekdpf lcnscgfcky akfdytltak pccavdpieg eedrkkavas insllekadk
     3841 vyrqlmahkp alellllkvq eqslvdrale eeaaaaaita tgaattgats atpantasas
     3901 gatcsmvnrs iqqlaqkycs eckasfdels kivqkilasr kelveyehkq kdsrkaqaat
     3961 ptvaavrpgr gelpspgnfs apagnkqssg rcygcasaav dhcitllral atnvpyrqll
     4021 cerglivelv ehnlrqgwsq vgqlvclltr dnpqatdhln sllmgritla llrggqpseq
     4081 pclavvpatr pttaddteaa atppvvvgvp dlpaavrhev allalslqke dscweqrlrc
     4141 vmrlflmgis sqspivlesi tlpclkvlqg iikpdppisk rnkdksieel atvrpsgmcv
     4201 avdlkgwldg dpvqtfsawk srclgrpadt lslgggkrqr redvrarflv ekyglrwrql
     4261 arrggssmrl lmllqppqqq paqglpcqpr plqsnwlrqi lfnpscrqar tvacslvkal
     4321 cqvpsrcrem ldllttyldd lgtagesgak flalyqslia pdhwkhylai kgllprlgnl
     4381 itaeieqltl leettlnadl sqgfalkmlt ellasfievd sirqqykarl vgcvlngyls
     4441 lrklvvqrtk lvdetqekll alleemttgt eseteafmav cvetlgrygl edlrtpvfif
     4501 erlcslihpe enelgeflvt lekdpqqedf lqgrmlgnpy sshepgmgpl mrdlknkvcq
     4561 dcelvalled dngmellvcn kimsldlpvk evyrkvwcpe ngeseamrvv yrmrgllgda
     4621 teefveslea kaggpqvdee qlyqlarvmg pcggleamle rldaiddlsr grplltvllk
     4681 lfglcvkvrs nrlhllepsl ravacllgal rlllgaqeaa laeqllatle avlaegaahv
     4741 ppqlpegvtk dditflldqv gtgrpsprll qllmrvvpfl tltdeakmdv linhfrrqln
     4801 firfdlehtp eddvqlecfc nlsagiernd ngnrlkdlli trgivegaiq yllvyappak
     4861 nsllsasenw keftskpalk yvlriltgls rghektqilv stecipiihr leqvssdehv
     4921 gslaenlmea lkenpnvaek ieevrkqtrd ekkrlamamr ekqlgelgmh tnekgqvtak
     4981 ssilkqmedl geeaglvcii cregykfqpg kllgiytftk rcnlddfeak prktpgystv
     5041 thfnivhvdc hmaavrharg rdewesaalq nantkcngll plwgpqvpes qfasclarhn
     5101 tyiqdctghl dvgymstlhd lklllsrfaf ersfsedsgg ggpqsnmhli pyllhmvlyv
     5161 inttrcvare eknlsnflem sperqvencf esegpcywat malavwshnr wqygraslvr
     5221 rmlilaharh lspqgcstlp dmvprefavy rpylcflgmv dglynimfkk vacstddgws
     5281 valadyirhn dqlhlelgdk llrtfeeqvl tcqsfrefcd ymgpmweidn pdaflhealq
     5341 lrv
//
LOCUS       XP_077505278             189 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115024 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505278
VERSION     XP_077505278.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649152.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..189
                     /product="uncharacterized protein LOC144115024 isoform X1"
                     /calculated_mol_wt=21465
     CDS             1..189
                     /gene="LOC144115024"
                     /coded_by="XM_077649152.1:1..570"
                     /db_xref="GeneID:144115024"
ORIGIN      
        1 mrivsiighl ywvaamgrgd gdlvasmwns llnhvcnkhn ghegpyqrci dgplekrqwm
       61 krnskafqel qkivssplll rdihqlspdv qtfslesfhs lliqfapkan afseegmqar
      121 tqlavmhyne nstkvqveta dgehrwkvkt skarkghftv cpvkeettys kqtivttrva
      181 afhrshqll
//
LOCUS       XP_077505279             187 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115024 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505279
VERSION     XP_077505279.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649153.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="uncharacterized protein LOC144115024 isoform X2"
                     /calculated_mol_wt=21071
     CDS             1..187
                     /gene="LOC144115024"
                     /coded_by="XM_077649153.1:1..564"
                     /db_xref="GeneID:144115024"
ORIGIN      
        1 mrivsiighl ywvaamgrgd gdlvasmwns llnhvcnkhn ghegpyqrci dgplekrqwm
       61 krnskafqel qkivssplll rdihqlspdv qtfslesfhs lliqfapkan afseegmqar
      121 tqlavmhyne nstkvqveta dgehrwkvkt skarkghftv cpvkeettys yvdnlllnav
      181 escegss
//
LOCUS       XP_077505280             692 aa            linear   INV 25-NOV-2025
DEFINITION  ATP-binding cassette sub-family F member 1 [Amblyomma americanum].
ACCESSION   XP_077505280
VERSION     XP_077505280.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649154.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..692
                     /product="ATP-binding cassette sub-family F member 1"
                     /calculated_mol_wt=77438
     Region          146..670
                     /region_name="Uup"
                     /note="ATPase components of ABC transporters with
                     duplicated ATPase domains [General function prediction
                     only]; COG0488"
                     /db_xref="CDD:440254"
     CDS             1..692
                     /gene="LOC144115028"
                     /coded_by="XM_077649154.1:198..2276"
                     /db_xref="GeneID:144115028"
ORIGIN      
        1 mppkkskaka akkeksgkgs segdvekrle dldisddngt dgddddcskp vrknkfqafg
       61 sdddvenseg sekeskgdda vkevtatkkv shkekklmkk qfeyqkqles lnkgktssdg
      121 dqftvsqadk saaqqmqlen aidikvdnfs isargkdlfv nasltitagr ryglvgpngh
      181 gkttllkhia nrslkippni dvllceqevv addtpavevv lkadvkrtel laeqkileed
      241 aargnlknqd rlsevyeelq ligadsaear arrilaglgf tremqdratk qfsggwrmrv
      301 slaralflep tlllldeptn hldlnaviwl dnylqvwkkt llvvshdqsf ldnvctdvih
      361 ldnqklfyyr gnfsqfkkmy vqrrkemike yekqekkikq mkasgmsskt atkttvqalt
      421 rkqqknkqkl qgteedngpa eliqrpkdyi vkfkfpnppp lnppilglyn vtfgypgqpl
      481 lfknldfgid mssrvaivgp ngvgkstflk llcgdltplq gearrnhrlr igrfdqhsge
      541 qlnsdespve ylqrlfnlny qdarkqlgsf glvshahtik nsdlsggqka rvalaelclr
      601 apdvlildep tnnldiesid aladaigdye ggviivshde rliretncql wviehktiee
      661 idgdfedyrk elleslgetv nspsiaarqa me
//
LOCUS       XP_077505281             417 aa            linear   INV 25-NOV-2025
DEFINITION  netrin receptor unc-40-like [Amblyomma americanum].
ACCESSION   XP_077505281
VERSION     XP_077505281.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649155.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..417
                     /product="netrin receptor unc-40-like"
                     /calculated_mol_wt=44000
     Region          <26..394
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     CDS             1..417
                     /gene="LOC144115029"
                     /coded_by="XM_077649155.1:248..1501"
                     /db_xref="GeneID:144115029"
ORIGIN      
        1 mqrgwsfals gaaayvllqi frlaaavgdg svgslsaglv gpdtamlswa qptggadgye
       61 lrvmrgsalv faqrfpldaa ektqpvlyyr ltglrpatty nvsvsaifgg qqgpvssvvf
      121 ttaalvsptt patasttqnt lngptsiqai plsstsvlls wgplapyvvg gqyrvrvldd
      181 enstrfldvg yvlsaivqgl datedytlsv qwcpsqnvcs afvstvrvan apklpvptnl
      241 tvvdvgassi nvtwqlpans annitgyqvs wcisdmcvgk lndltgstsy svqrlqdfkn
      301 ytvfvcayld rglttfvgqc vnvsaktqpa aptggvaipl sqssilviwa pkpgcpsisa
      361 yqlrvasptp tvnwvstemt qytfanlqsk tsytfqlrwc plpgtcsmsv nitgstv
//
LOCUS       XP_077505282             404 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115030 [Amblyomma americanum].
ACCESSION   XP_077505282
VERSION     XP_077505282.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649156.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..404
                     /product="uncharacterized protein LOC144115030"
                     /calculated_mol_wt=42748
     CDS             1..404
                     /gene="LOC144115030"
                     /coded_by="XM_077649156.1:528..1742"
                     /db_xref="GeneID:144115030"
ORIGIN      
        1 maavrqmsvp qaglepdera rspsrderrg sssprrsavi aestvvvaep lsksasqpss
       61 fkvkltrrdt snklavdsta akpttsssdg ergktkpnka agwktskffs ktmssaellr
      121 glkklsvnta ttstsddpea kpssggkvkf tmkkrnsssd savnkdrspe glrsgegsrk
      181 ssressdgsp nvsprtside givckkedil slihhntkkk ssigsvtvpv agatspkkla
      241 lrttrsatid psgmatdpye ptaenipriv stppaprrgs ttlerstsee akpskskrns
      301 lsgkgapqrs lddapqspad epsvgrkakr kqsgdavrea kketpeegvk dpakkdesqv
      361 kwdegnvvda mllgdaieaf lrgsmggsaa tmpatpkrvs fkkt
//
LOCUS       XP_077505283             516 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: protein O-mannosyltransferase 1-like
            [Amblyomma americanum].
ACCESSION   XP_077505283
VERSION     XP_077505283.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649157.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..516
                     /product="LOW QUALITY PROTEIN: protein
                     O-mannosyltransferase 1-like"
                     /calculated_mol_wt=59945
     Region          <2..57
                     /region_name="PMT_2"
                     /note="Dolichyl-phosphate-mannose-protein
                     mannosyltransferase; cl21590"
                     /db_xref="CDD:473917"
     Region          89..279
                     /region_name="beta-trefoil_MIR_POMT1"
                     /note="MIR domain, beta-trefoil fold, found in protein
                     O-mannosyl-transferase 1 (POMT1) and similar proteins;
                     cd23281"
                     /db_xref="CDD:467752"
     Site            order(190,243,245,264,269)
                     /site_type="other"
                     /note="putative heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:467752"
     Region          309..507
                     /region_name="PMT_4TMC"
                     /note="C-terminal four TMM region of
                     protein-O-mannosyltransferase; pfam16192"
                     /db_xref="CDD:465056"
     CDS             1..516
                     /gene="LOC144115031"
                     /coded_by="XM_077649157.1:88..1638"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 2 bases in 1 codon"
                     /db_xref="GeneID:144115031"
ORIGIN      
        1 mflavyimar dfwkmlgnss isdaklwahf iaraavmtvv plgiylgvfy lhlnlltrag
       61 phdnimtsaf qaslegglas itrgqplsvv hgsqitlrht hgracwlhsh phvypvkypd
      121 grgsshqqqv tcysfkdvnn wwivkrpsik divvsdppdv vkhgdivqli hgmtsralns
      181 hdvaspmspq hqevscyiny nisfpsqnlw rvdivnrdte gdvwhtihsh vrlihvntsq
      241 alkfsgkqlp dwgfhqhevv tdrfisqddt vwnveehryt knsdekerer dmvsaefvpl
      301 qpthltffqk mwelqykmll vnqenvqdhv yssepmewpl mirgiaywis pvtnaqihli
      361 gnlvtwytat iclflymlif cfyllrrqrl vydipedtfe kfkfggelcv vgyamhlvpy
      421 ffadralflh hylpalmyki lglvvvlehl dyvfchiirk kwiqlgfyaa vviwllsviy
      481 vfwkfsvfsy gttalsaqdv ldlkwkdtws fiihrp
//
LOCUS       XP_077505284             788 aa            linear   INV 25-NOV-2025
DEFINITION  protein O-mannosyltransferase rt [Amblyomma americanum].
ACCESSION   XP_077505284
VERSION     XP_077505284.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649158.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..788
                     /product="protein O-mannosyltransferase rt"
                     /calculated_mol_wt=90136
     Region          96..329
                     /region_name="PMT"
                     /note="Dolichyl-phosphate-mannose-protein
                     mannosyltransferase; pfam02366"
                     /db_xref="CDD:396786"
     Region          361..551
                     /region_name="beta-trefoil_MIR_POMT1"
                     /note="MIR domain, beta-trefoil fold, found in protein
                     O-mannosyl-transferase 1 (POMT1) and similar proteins;
                     cd23281"
                     /db_xref="CDD:467752"
     Site            order(462,515,517,536,541)
                     /site_type="other"
                     /note="putative heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:467752"
     Region          581..779
                     /region_name="PMT_4TMC"
                     /note="C-terminal four TMM region of
                     protein-O-mannosyltransferase; pfam16192"
                     /db_xref="CDD:465056"
     CDS             1..788
                     /gene="rt"
                     /coded_by="XM_077649158.1:182..2548"
                     /db_xref="GeneID:144115032"
ORIGIN      
        1 mseaknrkrn takaatasas lspvdafdgn ddpsnangtl hhedalkeis qvrceeinsa
       61 pgylsswlng csksfafnie idamstalfl lalltrmwrl eqprsivfde lhygkfasly
      121 mknifffdsh pplgkqlval agylagfdge tefdriggsy spsvplwalr fvpalfgsll
      181 vpvtyhlals lglaqlsavl aavlvlfens lltqsrfvlm eailmcfsal gllallkfrr
      241 qrsftfswwf wlilsvvflt cslcvkyiga fsmflavyim ardfwkmlgn ssisdaklwa
      301 hfiaraavmt vvplgiylgv fylhlnlltr agphdnimts afqasleggl asitrgqpls
      361 vvhgsqitlr hthgracwlh shphvypvky pdgrgsshqq qvtcysfkdv nnwwivkrps
      421 ikdivvsdpp dvvkhgdivq lihgmtsral nshdvaspms pqhqevscyi nynisfpsqn
      481 lwrvdivnrd tegdvwhtih shvrlihvnt sqalkfsgkq lpdwgfhqhe vvtdrfisqd
      541 dtvwnveehr ytknsdeker erdmvsaefv plqpthltff qkmwelqykm llvnqenvqd
      601 hvyssepmew plmirgiayw ispvtnaqih lignlvtwyt aticlflyml ifcfyllrrq
      661 rlvydipedt fekfkfggel cvvgyamhlv pyffadrtlf lhhylpalmy kilglvvvle
      721 hldyvfchii rkkwiqlgfy aavviwllsv iyvfwkfsvf sygttalsaq dvldlkwkdt
      781 wsfiihrp
//
LOCUS       XP_077505285             289 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115033 [Amblyomma americanum].
ACCESSION   XP_077505285
VERSION     XP_077505285.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649159.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..289
                     /product="uncharacterized protein LOC144115033"
                     /calculated_mol_wt=32634
     CDS             1..289
                     /gene="LOC144115033"
                     /coded_by="XM_077649159.1:2018..2887"
                     /db_xref="GeneID:144115033"
ORIGIN      
        1 mvsfviqppe afcfttpaaw pkwrkwfehf ytasglcdks gshqvntlly lmsdqaedil
       61 gtfklsgens qniqvvlkqf dsyfipwrnv ifewarfntr vqndgesved fvialhklsn
      121 hceygtlree lvrdrlvvsi qdqklsarlq ldadltlhka lefikkvksv rqqqaelhpd
      181 tltvnklasg rqqvghstnt tnvndgrehf esrphprpad nvptlclqcg kerhphslcp
      241 artqvckgch kkghytsvcm shrvdiveas gpdseggfvg tvlsfntlt
//
LOCUS       XP_077505286             112 aa            linear   INV 25-NOV-2025
DEFINITION  gem-associated protein 7-like [Amblyomma americanum].
ACCESSION   XP_077505286
VERSION     XP_077505286.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649160.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..112
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..112
                     /product="gem-associated protein 7-like"
                     /calculated_mol_wt=11998
     Region          31..100
                     /region_name="Sm_like"
                     /note="Sm and related proteins; cl00259"
                     /db_xref="CDD:469694"
     Site            order(45,63,75,86..98)
                     /site_type="other"
                     /note="heptamer interface [polypeptide binding]"
                     /db_xref="CDD:212462"
     Site            order(48..52,56..66,71..75)
                     /site_type="other"
                     /note="Sm1 motif"
                     /db_xref="CDD:212462"
     Site            order(51..52,56,58,64..65,71,73,86..96)
                     /site_type="other"
                     /note="hexamer interface [polypeptide binding]"
                     /db_xref="CDD:212462"
     Site            order(70..71,90,92)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:212462"
     Site            86..97
                     /site_type="other"
                     /note="Sm2 motif"
                     /db_xref="CDD:212462"
     CDS             1..112
                     /gene="LOC144115034"
                     /coded_by="XM_077649160.1:157..495"
                     /db_xref="GeneID:144115034"
ORIGIN      
        1 mppqgeesle slpkeekdge laarqqaara ylreqfllcm kglkdhvatf hmhenttvta
       61 tlrscdsdmq nfgvselsta lgtqpaalfr agdvisftvk dlaaatdsrv ga
//
LOCUS       XP_077505287             816 aa            linear   INV 25-NOV-2025
DEFINITION  DENN domain-containing protein 1B-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505287
VERSION     XP_077505287.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649161.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..816
                     /product="DENN domain-containing protein 1B-like isoform
                     X1"
                     /calculated_mol_wt=91457
     Region          9..82
                     /region_name="uDENN"
                     /note="Domain always found upstream of DENN domain, found
                     in a variety of signalling proteins; smart00800"
                     /db_xref="CDD:214824"
     Region          89..272
                     /region_name="DENN"
                     /note="Domain found in a variety of signalling proteins,
                     always encircled by uDENN and dDENN; smart00799"
                     /db_xref="CDD:214823"
     Region          304..369
                     /region_name="dDENN"
                     /note="Domain always found downstream of DENN domain,
                     found in a variety of signalling proteins; smart00801"
                     /db_xref="CDD:129037"
     Region          485..>809
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     CDS             1..816
                     /gene="LOC144115036"
                     /coded_by="XM_077649161.1:139..2589"
                     /db_xref="GeneID:144115036"
ORIGIN      
        1 mgsrirdnpa tiiecfcevv iprngnesaw ivqkfpdsyn neevlksvpe faypcefsst
       61 avqhfsfvlt nidskwtfgy crhapnsqtc fvllsylpwh etfykvlnqi aelankkdss
      121 lmghflesly stqvpepgfq lrvvydtdkn pfqcacsdht klpsipenrn lteyfnavda
      181 tnmivlfasm lherrilits rrlsrlsacv qaanaliypm ewqhifipvl pnhlldylsa
      241 pmpfligvpa vtlarvsrse lgevvildvd nnkvespfnd vetlpseivs plkrslrthh
      301 qtlgdgvsqa flralvalmg gyreavrmev gqritfdpdr flqsrpaslr pflermlqlq
      361 ifhqfiegrl qilnscevhn difelevnmf dessnkqfkq hykywlanmk keggallksm
      421 kskanpavrn ayknvrkqlr ksvvryttwy twsplphdvp revkdkgrka ytniqgkian
      481 mqkgtqddma lsrrdssqpr sapasptmdr krpvtfqplh rvsfsqeqpn asglvsplkt
      541 tsletstrtp vksptepnld tvaelseetg ssssfqhidm dlmgdlqeif srcattnlqi
      601 qpskvsqlvs pqapqekkaf sdpslhkvpl appprkaqrn tnrpspstda plirldsqen
      661 edelnnhqpp vvseanavav laplpaqltr vtqpgvqvaq plvppvqpav qmmaprmqal
      721 pgqlpltasp aafsghpfap spysnmqrrf qavpymgaph psfstnpflr mpwtppvtpp
      781 vtppvtsrpp tvpvsqppps smfpkqqdpc adlidl
//
LOCUS       XP_077505288             808 aa            linear   INV 25-NOV-2025
DEFINITION  DENN domain-containing protein 1A-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505288
VERSION     XP_077505288.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649162.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..808
                     /product="DENN domain-containing protein 1A-like isoform
                     X2"
                     /calculated_mol_wt=90554
     Region          9..82
                     /region_name="uDENN"
                     /note="Domain always found upstream of DENN domain, found
                     in a variety of signalling proteins; smart00800"
                     /db_xref="CDD:214824"
     Region          89..272
                     /region_name="DENN"
                     /note="Domain found in a variety of signalling proteins,
                     always encircled by uDENN and dDENN; smart00799"
                     /db_xref="CDD:214823"
     Region          304..369
                     /region_name="dDENN"
                     /note="Domain always found downstream of DENN domain,
                     found in a variety of signalling proteins; smart00801"
                     /db_xref="CDD:129037"
     Region          485..>801
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     CDS             1..808
                     /gene="LOC144115036"
                     /coded_by="XM_077649162.1:139..2565"
                     /db_xref="GeneID:144115036"
ORIGIN      
        1 mgsrirdnpa tiiecfcevv iprngnesaw ivqkfpdsyn neevlksvpe faypcefsst
       61 avqhfsfvlt nidskwtfgy crhapnsqtc fvllsylpwh etfykvlnqi aelankkdss
      121 lmghflesly stqvpepgfq lrvvydtdkn pfqcacsdht klpsipenrn lteyfnavda
      181 tnmivlfasm lherrilits rrlsrlsacv qaanaliypm ewqhifipvl pnhlldylsa
      241 pmpfligvpa vtlarvsrse lgevvildvd nnkvespfnd vetlpseivs plkrslrthh
      301 qtlgdgvsqa flralvalmg gyreavrmev gqritfdpdr flqsrpaslr pflermlqlq
      361 ifhqfiegrl qilnscevhn difelevnmf dessnkqfkq hykywlanmk keggallksm
      421 kskanpavrn ayknvrkqlr ksvvryttwy twsplphdvp revkdkgrka ytniqgkian
      481 mqkgtqddma lsrrdssqpr sapasptmdr krpvtfqplh rnasglvspl kttsletstr
      541 tpvksptepn ldtvaelsee tgssssfqhi dmdlmgdlqe ifsrcattnl qiqpskvsql
      601 vspqapqekk afsdpslhkv plappprkaq rntnrpspst daplirldsq enedelnnhq
      661 ppvvseanav avlaplpaql trvtqpgvqv aqplvppvqp avqmmaprmq alpgqlplta
      721 spaafsghpf apspysnmqr rfqavpymga phpsfstnpf lrmpwtppvt ppvtppvtsr
      781 pptvpvsqpp pssmfpkqqd pcadlidl
//
LOCUS       XP_077505289             788 aa            linear   INV 25-NOV-2025
DEFINITION  DENN domain-containing protein 1A-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505289
VERSION     XP_077505289.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649163.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..788
                     /product="DENN domain-containing protein 1A-like isoform
                     X3"
                     /calculated_mol_wt=87976
     Region          9..82
                     /region_name="uDENN"
                     /note="Domain always found upstream of DENN domain, found
                     in a variety of signalling proteins; smart00800"
                     /db_xref="CDD:214824"
     Region          89..272
                     /region_name="DENN"
                     /note="Domain found in a variety of signalling proteins,
                     always encircled by uDENN and dDENN; smart00799"
                     /db_xref="CDD:214823"
     Region          304..369
                     /region_name="dDENN"
                     /note="Domain always found downstream of DENN domain,
                     found in a variety of signalling proteins; smart00801"
                     /db_xref="CDD:129037"
     Region          457..>781
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     CDS             1..788
                     /gene="LOC144115036"
                     /coded_by="XM_077649163.1:139..2505"
                     /db_xref="GeneID:144115036"
ORIGIN      
        1 mgsrirdnpa tiiecfcevv iprngnesaw ivqkfpdsyn neevlksvpe faypcefsst
       61 avqhfsfvlt nidskwtfgy crhapnsqtc fvllsylpwh etfykvlnqi aelankkdss
      121 lmghflesly stqvpepgfq lrvvydtdkn pfqcacsdht klpsipenrn lteyfnavda
      181 tnmivlfasm lherrilits rrlsrlsacv qaanaliypm ewqhifipvl pnhlldylsa
      241 pmpfligvpa vtlarvsrse lgevvildvd nnkvespfnd vetlpseivs plkrslrthh
      301 qtlgdgvsqa flralvalmg gyreavrmev gqritfdpdr flqsrpaslr pflermlqlq
      361 ifhqfiegrl qilnscevhn difelevnmf dessnkqfkq hykywlanmk keggallksm
      421 kskanpavrn ayknvkdkgr kaytniqgki anmqkgtqdd malsrrdssq prsapasptm
      481 drkrpvtfqp lhrvsfsqeq pnasglvspl kttsletstr tpvksptepn ldtvaelsee
      541 tgssssfqhi dmdlmgdlqe ifsrcattnl qiqpskvsql vspqapqekk afsdpslhkv
      601 plappprkaq rntnrpspst daplirldsq enedelnnhq ppvvseanav avlaplpaql
      661 trvtqpgvqv aqplvppvqp avqmmaprmq alpgqlplta spaafsghpf apspysnmqr
      721 rfqavpymga phpsfstnpf lrmpwtppvt ppvtppvtsr pptvpvsqpp pssmfpkqqd
      781 pcadlidl
//
LOCUS       XP_077505290             780 aa            linear   INV 25-NOV-2025
DEFINITION  DENN domain-containing protein 1A-like isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505290
VERSION     XP_077505290.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649164.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..780
                     /product="DENN domain-containing protein 1A-like isoform
                     X4"
                     /calculated_mol_wt=87073
     Region          9..82
                     /region_name="uDENN"
                     /note="Domain always found upstream of DENN domain, found
                     in a variety of signalling proteins; smart00800"
                     /db_xref="CDD:214824"
     Region          89..272
                     /region_name="DENN"
                     /note="Domain found in a variety of signalling proteins,
                     always encircled by uDENN and dDENN; smart00799"
                     /db_xref="CDD:214823"
     Region          304..369
                     /region_name="dDENN"
                     /note="Domain always found downstream of DENN domain,
                     found in a variety of signalling proteins; smart00801"
                     /db_xref="CDD:129037"
     Region          457..>773
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     CDS             1..780
                     /gene="LOC144115036"
                     /coded_by="XM_077649164.1:146..2488"
                     /db_xref="GeneID:144115036"
ORIGIN      
        1 mgsrirdnpa tiiecfcevv iprngnesaw ivqkfpdsyn neevlksvpe faypcefsst
       61 avqhfsfvlt nidskwtfgy crhapnsqtc fvllsylpwh etfykvlnqi aelankkdss
      121 lmghflesly stqvpepgfq lrvvydtdkn pfqcacsdht klpsipenrn lteyfnavda
      181 tnmivlfasm lherrilits rrlsrlsacv qaanaliypm ewqhifipvl pnhlldylsa
      241 pmpfligvpa vtlarvsrse lgevvildvd nnkvespfnd vetlpseivs plkrslrthh
      301 qtlgdgvsqa flralvalmg gyreavrmev gqritfdpdr flqsrpaslr pflermlqlq
      361 ifhqfiegrl qilnscevhn difelevnmf dessnkqfkq hykywlanmk keggallksm
      421 kskanpavrn ayknvkdkgr kaytniqgki anmqkgtqdd malsrrdssq prsapasptm
      481 drkrpvtfqp lhrnasglvs plkttslets trtpvkspte pnldtvaels eetgssssfq
      541 hidmdlmgdl qeifsrcatt nlqiqpskvs qlvspqapqe kkafsdpslh kvplappprk
      601 aqrntnrpsp stdaplirld sqenedelnn hqppvvsean avavlaplpa qltrvtqpgv
      661 qvaqplvppv qpavqmmapr mqalpgqlpl taspaafsgh pfapspysnm qrrfqavpym
      721 gaphpsfstn pflrmpwtpp vtppvtppvt srpptvpvsq pppssmfpkq qdpcadlidl
//
LOCUS       XP_077505292             173 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115038 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505292
VERSION     XP_077505292.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649166.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..173
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..173
                     /product="uncharacterized protein LOC144115038 isoform X1"
                     /calculated_mol_wt=19823
     Region          101..148
                     /region_name="DUF5522"
                     /note="Family of unknown function (DUF5522); pfam17653"
                     /db_xref="CDD:435947"
     CDS             1..173
                     /gene="LOC144115038"
                     /coded_by="XM_077649166.1:73..594"
                     /db_xref="GeneID:144115038"
ORIGIN      
        1 mfgsaccalk srpnlqhgsl ivsqlfsils kmlarvlarr crryqlarcf wtsaeedanp
       61 gpkteeerrk ktaeyskifh dmdvsvcwgr lsdnerkiye ahklaiqrae ksyidpvtgf
      121 nvftglfhyl rgyccgfgcr hcpyqhinak devkekmmfn safyiprtma sgs
//
LOCUS       XP_077505293             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein C1orf53-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505293
VERSION     XP_077505293.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649167.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein C1orf53-like isoform X2"
                     /calculated_mol_wt=16029
     Region          69..116
                     /region_name="DUF5522"
                     /note="Family of unknown function (DUF5522); pfam17653"
                     /db_xref="CDD:435947"
     CDS             1..141
                     /gene="LOC144115038"
                     /coded_by="XM_077649167.1:25..450"
                     /db_xref="GeneID:144115038"
ORIGIN      
        1 maasvcfmic cesgdvrlcm laeedanpgp kteeerrkkt aeyskifhdm dvsvcwgrls
       61 dnerkiyeah klaiqraeks yidpvtgfnv ftglfhylrg yccgfgcrhc pyqhinakde
      121 vkekmmfnsa fyiprtmasg s
//
LOCUS       XP_077505294             175 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115041 [Amblyomma americanum].
ACCESSION   XP_077505294
VERSION     XP_077505294.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649168.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..175
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..175
                     /product="uncharacterized protein LOC144115041"
                     /calculated_mol_wt=19616
     CDS             1..175
                     /gene="LOC144115041"
                     /coded_by="XM_077649168.1:410..937"
                     /db_xref="GeneID:144115041"
ORIGIN      
        1 mlasklcdls dlddgsalkl fealgsavqq lsderislnd reraakttie almgerdalm
       61 aenrvfaekq kdynelqqak esledhltih kdvaknitre vhhqlttvkd tlkqviqesg
      121 iaqgpfeatl qtvvreamkl rtqiflfydh wshsqgvtsn msdelarkks setls
//
LOCUS       XP_077505295             708 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115043 [Amblyomma americanum].
ACCESSION   XP_077505295
VERSION     XP_077505295.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649169.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..708
                     /product="uncharacterized protein LOC144115043"
                     /calculated_mol_wt=76305
     CDS             1..708
                     /gene="LOC144115043"
                     /coded_by="XM_077649169.1:50..2176"
                     /db_xref="GeneID:144115043"
ORIGIN      
        1 maspgasslv kfnirwgnrs nqssimntse yidtlkkrlr avimknkila ralaaskkei
       61 selkksngrq qkvfnlglpq vktwlkmarq hshtmyqyqs raltlldeim npgvdftlar
      121 vsdadddadi qdrrpslhpp gkrskpqrge lsvipeeded vnasrrlllg tftldsetrl
      181 raaetierks rgsmssserp ssssgkprap tpkgsvqslg ckvrgpwwtd ggagdldyaf
      241 lespnptapl rqgnepgekm vtvdaqsrip evadpqhqet asekpsssts tadldnapre
      301 sflpnkrkee adgqefvglk padtlvanga eaygvdqrsp rqinrhppra lchmsspspg
      361 esedttafgf aevigrnirr gkktgitprd fdlsslgsps liaavpkfpe lpsphkvtta
      421 saedsnskel rtshqkfpev lptlpssahs ekvprlripp nkekeptngp dldslvpays
      481 pvanggeesc gidqrspqqi srhasrsspe haedstglyf lrligrnitc rrrtgitscd
      541 ldlsslesps paaavpeisd lasaqmvtta tgqeksshev gtasqkapde pptlasrtds
      601 ekgshasfar skenepaamt glggmgpsth paatiepvpg vdqgnsrptk rqgaktsrrr
      661 garretsedg ttssvasstv dgesvarsts grplrsaaig knyresdi
//
LOCUS       XP_077505296             151 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115044 [Amblyomma americanum].
ACCESSION   XP_077505296
VERSION     XP_077505296.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649170.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..151
                     /product="uncharacterized protein LOC144115044"
                     /calculated_mol_wt=16036
     CDS             1..151
                     /gene="LOC144115044"
                     /coded_by="XM_077649170.1:673..1128"
                     /db_xref="GeneID:144115044"
ORIGIN      
        1 mdfmaspvdd tpspeigwst igasrkpasa trprselitv giqlppgtln pklplydvla
       61 avtstaqlss ktcaditlqa rpaqslvflk thspltahll lsithlhveg lqqptlhgas
      121 fsgprhlfpv pgywlkivpt slhlglqaks h
//
LOCUS       XP_077505297             151 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115044 [Amblyomma americanum].
ACCESSION   XP_077505297
VERSION     XP_077505297.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649171.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..151
                     /product="uncharacterized protein LOC144115044"
                     /calculated_mol_wt=16036
     CDS             1..151
                     /gene="LOC144115044"
                     /coded_by="XM_077649171.1:611..1066"
                     /db_xref="GeneID:144115044"
ORIGIN      
        1 mdfmaspvdd tpspeigwst igasrkpasa trprselitv giqlppgtln pklplydvla
       61 avtstaqlss ktcaditlqa rpaqslvflk thspltahll lsithlhveg lqqptlhgas
      121 fsgprhlfpv pgywlkivpt slhlglqaks h
//
LOCUS       XP_077505298             151 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115044 [Amblyomma americanum].
ACCESSION   XP_077505298
VERSION     XP_077505298.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649172.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..151
                     /product="uncharacterized protein LOC144115044"
                     /calculated_mol_wt=16036
     CDS             1..151
                     /gene="LOC144115044"
                     /coded_by="XM_077649172.1:731..1186"
                     /db_xref="GeneID:144115044"
ORIGIN      
        1 mdfmaspvdd tpspeigwst igasrkpasa trprselitv giqlppgtln pklplydvla
       61 avtstaqlss ktcaditlqa rpaqslvflk thspltahll lsithlhveg lqqptlhgas
      121 fsgprhlfpv pgywlkivpt slhlglqaks h
//
LOCUS       XP_077505299             151 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115046 [Amblyomma americanum].
ACCESSION   XP_077505299
VERSION     XP_077505299.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649173.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..151
                     /product="uncharacterized protein LOC144115046"
                     /calculated_mol_wt=16036
     CDS             1..151
                     /gene="LOC144115046"
                     /coded_by="XM_077649173.1:673..1128"
                     /db_xref="GeneID:144115046"
ORIGIN      
        1 mdfmaspvdd tpspeigwst igasrkpasa trprselitv giqlppgtln pklplydvla
       61 avtstaqlss ktcaditlqa rpaqslvflk thspltahll lsithlhveg lqqptlhgas
      121 fsgprhlfpv pgywlkivpt slhlglqaks h
//
LOCUS       XP_077505300             151 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115046 [Amblyomma americanum].
ACCESSION   XP_077505300
VERSION     XP_077505300.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649174.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..151
                     /product="uncharacterized protein LOC144115046"
                     /calculated_mol_wt=16036
     CDS             1..151
                     /gene="LOC144115046"
                     /coded_by="XM_077649174.1:611..1066"
                     /db_xref="GeneID:144115046"
ORIGIN      
        1 mdfmaspvdd tpspeigwst igasrkpasa trprselitv giqlppgtln pklplydvla
       61 avtstaqlss ktcaditlqa rpaqslvflk thspltahll lsithlhveg lqqptlhgas
      121 fsgprhlfpv pgywlkivpt slhlglqaks h
//
LOCUS       XP_077505301             151 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115046 [Amblyomma americanum].
ACCESSION   XP_077505301
VERSION     XP_077505301.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649175.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..151
                     /product="uncharacterized protein LOC144115046"
                     /calculated_mol_wt=16036
     CDS             1..151
                     /gene="LOC144115046"
                     /coded_by="XM_077649175.1:731..1186"
                     /db_xref="GeneID:144115046"
ORIGIN      
        1 mdfmaspvdd tpspeigwst igasrkpasa trprselitv giqlppgtln pklplydvla
       61 avtstaqlss ktcaditlqa rpaqslvflk thspltahll lsithlhveg lqqptlhgas
      121 fsgprhlfpv pgywlkivpt slhlglqaks h
//
LOCUS       XP_077505302             151 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115048 [Amblyomma americanum].
ACCESSION   XP_077505302
VERSION     XP_077505302.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649176.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..151
                     /product="uncharacterized protein LOC144115048"
                     /calculated_mol_wt=16036
     CDS             1..151
                     /gene="LOC144115048"
                     /coded_by="XM_077649176.1:673..1128"
                     /db_xref="GeneID:144115048"
ORIGIN      
        1 mdfmaspvdd tpspeigwst igasrkpasa trprselitv giqlppgtln pklplydvla
       61 avtstaqlss ktcaditlqa rpaqslvflk thspltahll lsithlhveg lqqptlhgas
      121 fsgprhlfpv pgywlkivpt slhlglqaks h
//
LOCUS       XP_077505303             151 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115048 [Amblyomma americanum].
ACCESSION   XP_077505303
VERSION     XP_077505303.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649177.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..151
                     /product="uncharacterized protein LOC144115048"
                     /calculated_mol_wt=16036
     CDS             1..151
                     /gene="LOC144115048"
                     /coded_by="XM_077649177.1:611..1066"
                     /db_xref="GeneID:144115048"
ORIGIN      
        1 mdfmaspvdd tpspeigwst igasrkpasa trprselitv giqlppgtln pklplydvla
       61 avtstaqlss ktcaditlqa rpaqslvflk thspltahll lsithlhveg lqqptlhgas
      121 fsgprhlfpv pgywlkivpt slhlglqaks h
//
LOCUS       XP_077505305             151 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115048 [Amblyomma americanum].
ACCESSION   XP_077505305
VERSION     XP_077505305.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649179.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..151
                     /product="uncharacterized protein LOC144115048"
                     /calculated_mol_wt=16036
     CDS             1..151
                     /gene="LOC144115048"
                     /coded_by="XM_077649179.1:731..1186"
                     /db_xref="GeneID:144115048"
ORIGIN      
        1 mdfmaspvdd tpspeigwst igasrkpasa trprselitv giqlppgtln pklplydvla
       61 avtstaqlss ktcaditlqa rpaqslvflk thspltahll lsithlhveg lqqptlhgas
      121 fsgprhlfpv pgywlkivpt slhlglqaks h
//
LOCUS       XP_077505306             725 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077505306
VERSION     XP_077505306.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649180.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..725
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=80048
     Region          72..198
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          298..690
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..725
                     /gene="LOC144115050"
                     /coded_by="XM_077649180.1:173..2350"
                     /db_xref="GeneID:144115050"
ORIGIN      
        1 mgqrpftpsl lfaamfsala llgarvgwaq gaaesatrsy eddlsevlkv avqkllplis
       61 dtvtrpevsi dcstsmiktf lalrqqkawa vrmamsnamy apnalegtyv amggfdqclr
      121 trvrlsdgel yfkgqycsvl fslpedyhrr fiesfhqige lqgrlnplta ssperegidt
      181 igglcspslc tkedltfitr slanvyggna tvtscstdna keiapvqafs iavlcilgll
      241 vgvgttlewl pqadlqasrl kpgvlmrfll vfsaitntkf llrtkvkkqn ealkfvcglk
      301 vimmfwvvlg hayivlpvgy fhavfamahm tehitfqfit naflsvtiff flsgfvlafs
      361 gvssqeavak ksifvvyfvr slrrytrlvi pavililvaf llplfgqgpa addafkqyil
      421 gcydkwwtvv lqannfddse hvclqhlwyi sadlqiflvv afplallflk qpraaaivga
      481 ltaavfclmt cvqiyswdlf yaftggtndm rkvkdtleli yfrpfthaaa yiagvmcgyv
      541 saqyknvtmg pnvemglwiv ssalacgvlf asfpwnrghl pdevtgalyg gfhrllwala
      601 ffwpsyacat srggllnkil swsffvplsr ltygiyllhv llymtrmlrl rtyintdeyf
      661 qlttalgvfs lsvvfafvlh lcveapvlrl ekllcdgdag dgvaappasg kasspqlgtt
      721 qkstv
//
LOCUS       XP_077505307             722 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077505307
VERSION     XP_077505307.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649181.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..722
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=81438
     Region          64..192
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          276..683
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..722
                     /gene="LOC144115051"
                     /coded_by="XM_077649181.1:306..2474"
                     /db_xref="GeneID:144115051"
ORIGIN      
        1 mssltkrstf aallctalvl gaaaeesatr syeadlrdia qvvikkffpi vselvtspel
       61 svdcstsllk tfsalrqqkg wayrmvmsna mfssnalegt yvamggydqc lrarayladg
      121 eldfkgqycs lfyklpkayf etfikrfhei geltgrknpl tatsshefqs idtrgalctp
      181 ssctaedmtf mirsifsmyg gnatvtscrt ddpkpltkvh viclailgsl lllvlvgtat
      241 eyvimkrypk kkvnasaplr allafsaitn twyllrtkvk perkslqfma glkvimafwv
      301 vfghsyilvd tgffhavytm efmtwnvmfq filnaflsvt tfffisgfll sylviesrsa
      361 vlknnlllvy isrglrrylr ltiptlvvlv aglllplmgs gpadedafnq hirgcysgww
      421 ktivlaenfv kegelcfrhl wyvstdlqif mliafpltll llkypktshv igaivavafc
      481 vftciqihtw dlfyaftggt ndmrriektl fyvyvrpfth vatyvvgvlc gyvasqhknk
      541 hihlmlqall wclslflvcl vmfitvpwnq gqlpddvtns iyggfhrvaw algffwptya
      601 catgrggwln kmfswkfflp lsrltfciyl vhvliyivrm irlrtyintd elfqlntalg
      661 vfalsivfgy alhilieapv fqlekiifer kaeqplpsag mngvangvdh anskdelkss
      721 il
//
LOCUS       XP_077505308             752 aa            linear   INV 25-NOV-2025
DEFINITION  cGMP-dependent protein kinase for isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505308
VERSION     XP_077505308.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649182.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..752
                     /product="cGMP-dependent protein kinase for isoform X1"
                     /calculated_mol_wt=84471
     Region          180..289
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(244..245,254..256)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Site            order(276..278,282..284)
                     /site_type="other"
                     /note="flexible hinge region"
                     /db_xref="CDD:237999"
     Region          299..415
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(372..373,382..384)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Site            order(406..408,412..414)
                     /site_type="other"
                     /note="flexible hinge region"
                     /db_xref="CDD:237999"
     Region          447..707
                     /region_name="STKc_cGK"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     cGMP-dependent protein kinase (cGK or PKG); cd05572"
                     /db_xref="CDD:270724"
     Site            order(447..451,455,469,471,503,519..520,522,526,528,565,
                     567,569..570,572,582..583,586,599..604,631,637,640)
                     /site_type="active"
                     /db_xref="CDD:270724"
     Site            order(447..451,455,469,471,503,520..522,526,565,567,
                     569..570,572,582..583)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270724"
     Site            order(451,526,528,565,567,569,586,599..604,631,637,640)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270724"
     Site            order(582..592,596..604)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270724"
     Region          701..>731
                     /region_name="S_TK_X"
                     /note="Extension to Ser/Thr-type protein kinases;
                     smart00133"
                     /db_xref="CDD:214529"
     CDS             1..752
                     /gene="for"
                     /coded_by="XM_077649182.1:115..2373"
                     /db_xref="GeneID:144115054"
ORIGIN      
        1 mlsavvpsas pcesrgsrcp ssashrrrrq lrssssprar rrqpqqvprr ghsssasavr
       61 sssgpvcfsw tpaslivgap sravdvqvat alwiaargqv atlvlaglqr gqeacerala
      121 slgaglralt allkgvlrvl mrighgygda csarlrtcll llrrarsrel ikqaiqdndf
      181 mknletvqik eitdcmypve yaqdsliike gdvgsvvyvm eegkvevtke skflctlgpg
      241 kvfgelaily nctrtatvka isdcklwaie rqcfqtimmr tglvrqseyt eflksvptfr
      301 klpeetlvki sdvleettya qgdyiirqga rgdtffiisk gtvkvtrkap eeagstaeei
      361 firtlhrgdf fgekalqgee crtaniiadd pegvtclvid rdsfnqlmsn ideirgkqyd
      421 edvgarqrin eefanlklsd lrviatlgvg gfgrvelvqs mqdpmrsfal kvmkkaqive
      481 trqqqhimse kmileeancd fiiklyktfk drkylymlle pclggelwti lrdrgnfddn
      541 ttrfytacvv eafdylharn iiyrdlkpen mlldisgyik lvdfgfakkl vngrktwtfc
      601 gtpeyvapev ilnrghdisa dywslgvlmf elltgappft dpdfmktyni ilkgidaidf
      661 prcitrnata likklcrdnp nerlgygkgg irdimkhkwf dgfnweglht rtlkppiipq
      721 vrhvtdtsnf dryppdtdgp ppddmtgwda nf
//
LOCUS       XP_077505309             718 aa            linear   INV 25-NOV-2025
DEFINITION  cGMP-dependent protein kinase for isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505309
VERSION     XP_077505309.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649183.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..718
                     /product="cGMP-dependent protein kinase for isoform X2"
                     /calculated_mol_wt=81979
     Region          41..88
                     /region_name="DD_cGKI-alpha"
                     /note="Dimerization/Docking domain of Cyclic GMP-dependent
                     Protein Kinase I alpha; cd12085"
                     /db_xref="CDD:213374"
     Site            order(41,43..44,47,50..51,54..55,58,61..62,65,68..69,72,
                     75..76,79..80,82..83,87)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:213374"
     Site            order(59..60,62,64)
                     /site_type="other"
                     /note="putative GKAP docking site [polypeptide binding]"
                     /db_xref="CDD:213374"
     Region          146..255
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(210..211,220..222)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Site            order(242..244,248..250)
                     /site_type="other"
                     /note="flexible hinge region"
                     /db_xref="CDD:237999"
     Region          265..381
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(338..339,348..350)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Site            order(372..374,378..380)
                     /site_type="other"
                     /note="flexible hinge region"
                     /db_xref="CDD:237999"
     Region          413..673
                     /region_name="STKc_cGK"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     cGMP-dependent protein kinase (cGK or PKG); cd05572"
                     /db_xref="CDD:270724"
     Site            order(413..417,421,435,437,469,485..486,488,492,494,531,
                     533,535..536,538,548..549,552,565..570,597,603,606)
                     /site_type="active"
                     /db_xref="CDD:270724"
     Site            order(413..417,421,435,437,469,486..488,492,531,533,
                     535..536,538,548..549)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270724"
     Site            order(417,492,494,531,533,535,552,565..570,597,603,606)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270724"
     Site            order(548..558,562..570)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270724"
     Region          667..>697
                     /region_name="S_TK_X"
                     /note="Extension to Ser/Thr-type protein kinases;
                     smart00133"
                     /db_xref="CDD:214529"
     CDS             1..718
                     /gene="for"
                     /coded_by="XM_077649183.1:183..2339"
                     /db_xref="GeneID:144115054"
ORIGIN      
        1 mfpsrprrsv viclsssaad rklecdespp psscpaalls mgtleelrda vlkqeriihd
       61 lrqvitlrde eilqlrsqld kfqsvlipyn arysrsgrrk eraqgisaep qslrsiqdli
      121 qtkfqeypks drsrelikqa iqdndfmknl etvqikeitd cmypveyaqd sliikegdvg
      181 svvyvmeegk vevtkeskfl ctlgpgkvfg elailynctr tatvkaisdc klwaierqcf
      241 qtimmrtglv rqseyteflk svptfrklpe etlvkisdvl eettyaqgdy iirqgargdt
      301 ffiiskgtvk vtrkapeeag staeeifirt lhrgdffgek alqgeecrta niiaddpegv
      361 tclvidrdsf nqlmsnidei rgkqydedvg arqrineefa nlklsdlrvi atlgvggfgr
      421 velvqsmqdp mrsfalkvmk kaqivetrqq qhimsekmil eeancdfiik lyktfkdrky
      481 lymllepclg gelwtilrdr gnfddnttrf ytacvveafd ylharniiyr dlkpenmlld
      541 isgyiklvdf gfakklvngr ktwtfcgtpe yvapevilnr ghdisadyws lgvlmfellt
      601 gappftdpdf mktyniilkg idaidfprci trnatalikk lcrdnpnerl gygkggirdi
      661 mkhkwfdgfn weglhtrtlk ppiipqvrhv tdtsnfdryp pdtdgpppdd mtgwdanf
//
LOCUS       XP_077505310             708 aa            linear   INV 25-NOV-2025
DEFINITION  cGMP-dependent protein kinase for isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505310
VERSION     XP_077505310.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649184.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..708
                     /product="cGMP-dependent protein kinase for isoform X3"
                     /calculated_mol_wt=80159
     Region          136..245
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(200..201,210..212)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Site            order(232..234,238..240)
                     /site_type="other"
                     /note="flexible hinge region"
                     /db_xref="CDD:237999"
     Region          255..371
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(328..329,338..340)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Site            order(362..364,368..370)
                     /site_type="other"
                     /note="flexible hinge region"
                     /db_xref="CDD:237999"
     Region          403..663
                     /region_name="STKc_cGK"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     cGMP-dependent protein kinase (cGK or PKG); cd05572"
                     /db_xref="CDD:270724"
     Site            order(403..407,411,425,427,459,475..476,478,482,484,521,
                     523,525..526,528,538..539,542,555..560,587,593,596)
                     /site_type="active"
                     /db_xref="CDD:270724"
     Site            order(403..407,411,425,427,459,476..478,482,521,523,
                     525..526,528,538..539)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270724"
     Site            order(407,482,484,521,523,525,542,555..560,587,593,596)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270724"
     Site            order(538..548,552..560)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270724"
     Region          657..>687
                     /region_name="S_TK_X"
                     /note="Extension to Ser/Thr-type protein kinases;
                     smart00133"
                     /db_xref="CDD:214529"
     CDS             1..708
                     /gene="for"
                     /coded_by="XM_077649184.1:1614..3740"
                     /db_xref="GeneID:144115054"
ORIGIN      
        1 mdrsccekna adggsvaelr aalekcrlel sekdgviadl rsrlelqegv srdlelqlgk
       61 fravlkpfte qlahnlrlsa gcqdqsslpa agarlkrlai saepvdvrle htpakkvpkd
      121 dtsrelikqa iqdndfmknl etvqikeitd cmypveyaqd sliikegdvg svvyvmeegk
      181 vevtkeskfl ctlgpgkvfg elailynctr tatvkaisdc klwaierqcf qtimmrtglv
      241 rqseyteflk svptfrklpe etlvkisdvl eettyaqgdy iirqgargdt ffiiskgtvk
      301 vtrkapeeag staeeifirt lhrgdffgek alqgeecrta niiaddpegv tclvidrdsf
      361 nqlmsnidei rgkqydedvg arqrineefa nlklsdlrvi atlgvggfgr velvqsmqdp
      421 mrsfalkvmk kaqivetrqq qhimsekmil eeancdfiik lyktfkdrky lymllepclg
      481 gelwtilrdr gnfddnttrf ytacvveafd ylharniiyr dlkpenmlld isgyiklvdf
      541 gfakklvngr ktwtfcgtpe yvapevilnr ghdisadyws lgvlmfellt gappftdpdf
      601 mktyniilkg idaidfprci trnatalikk lcrdnpnerl gygkggirdi mkhkwfdgfn
      661 weglhtrtlk ppiipqvrhv tdtsnfdryp pdtdgpppdd mtgwdanf
//
LOCUS       XP_077505311             630 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115055 [Amblyomma americanum].
ACCESSION   XP_077505311
VERSION     XP_077505311.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649185.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..630
                     /product="uncharacterized protein LOC144115055"
                     /calculated_mol_wt=71462
     CDS             1..630
                     /gene="LOC144115055"
                     /coded_by="XM_077649185.1:310..2202"
                     /db_xref="GeneID:144115055"
ORIGIN      
        1 mfpdsetakg fqcgrkklsy iisdglgpyf kakviqeldm pdtfytimid espipeakvq
       61 qldvlvryys tstenvvveh lqsfhlghat adqlfscied alsdvrkknm vcfysdgpnv
      121 mkslkrrlkk evspdmvdig ecglhkvhna faagldmfca eveslatdvh yyfkfatrha
      181 dmkellsdlg lpqleflrhv nsrwltllps ierilksfda lkaffsksgq prcssmrhgr
      241 lssafcdktl raklfflqna aqifdrfqtl fqskdpllhv fydemlvlvk qvlgrflrqe
      301 sfagatgsql keldvessen wkakpeigld teqsmklwnp tekkafyika rafyiacaky
      361 litrlpldnk llfhlrflnp dtkgnsftss lryvanalpq vippcdvssl tdewnslmce
      421 tsdwelppnv vthwssvfal qtpagqakyp ritklvkaal slphgnadce rgfsenkqal
      481 hhrstlsits isslrqtkaf mkrysgdatk vsltrdilrn veksykvyre rieeektats
      541 qkrkheeeep tevcerkklm deksslqqrl sslkallasa qeliskgvad rdmdkvesgn
      601 illcdvnskl psvieriktv dsalqsmkan
//
LOCUS       XP_077505312             281 aa            linear   INV 25-NOV-2025
DEFINITION  elongation of very long chain fatty acids protein baldspot
            [Amblyomma americanum].
ACCESSION   XP_077505312
VERSION     XP_077505312.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649186.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..281
                     /product="elongation of very long chain fatty acids
                     protein baldspot"
                     /calculated_mol_wt=32850
     Region          37..277
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..281
                     /gene="Baldspot"
                     /coded_by="XM_077649186.1:63..908"
                     /db_xref="GeneID:144115056"
ORIGIN      
        1 mggvgveapl nllnikpnys fefdfevnfd kdqnrlwmnr nwhtsvywvg lymlvvfggq
       61 aymaqrpafk ltrplqawnv llsvfsvvga crtipelihv lrefgffhsv cnnsyieydr
      121 vsgfwtwmfv lskvpelgdt vfiilrkkel iflhwyhhit vlmfswyfyq qhiaparwyv
      181 vmnyvvhslm ysyfalrsfr vrmprwigvc itslqigqml mgayvtglgf ftnkrgaasc
      241 aisertalla mfmylsyfil falffygayl kpkrkvaaka d
//
LOCUS       XP_077505313            1235 aa            linear   INV 25-NOV-2025
DEFINITION  protein transport protein Sec24AB isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505313
VERSION     XP_077505313.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649187.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1235
                     /product="protein transport protein Sec24AB isoform X1"
                     /calculated_mol_wt=132488
     Region          <17..441
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          383..1235
                     /region_name="COG5028"
                     /note="Vesicle coat complex COPII, subunit SEC24/subunit
                     SFB2/subunit SFB3 [Intracellular trafficking and
                     secretion]"
                     /db_xref="CDD:227361"
     CDS             1..1235
                     /gene="Sec24AB"
                     /coded_by="XM_077649187.1:14..3721"
                     /db_xref="GeneID:144115057"
ORIGIN      
        1 mqgmampggq gvpyggaipy grpgppasgv aegisphavp ygdsahhvak eaqllqapps
       61 agaaqlppmq skpngptvng swthlgggyt ngpslkvssh qqqvpssspa gvassrvnqg
      121 yqqpgssplv tptaseptsa kssrnaspip sqrydfmegq fssprpspis ymhpstsqaa
      181 ppvrgppppa gffpptpqgs nmppsarivp qpgppligqp pvsgtslqrp tpvsgpigpp
      241 pptsmsgsty pvsnpssafp gpptsgvhaa ssnqhlaagt qpsfqgqppl ptsfrpvsgp
      301 ppvpgphtlp ganrvpgppa avpptsvpaq pssqfpppss gvsgasfpgp rtaglagptm
      361 agpgmapasv sqpgthtqgf gpqppqgstl gapqqrpgyp gppptsgapa qlqtlhgrps
      421 ypqqfgysqs vgnssmpppp plynaqqqpm qqppleppig gapgydpelh srmaglsvna
      481 sfnklwgyea cnllqsrnil pkapedppkp slpgenancs tdifrctltk ipetqsllqk
      541 arlplgvlih pfrdvshlpv iqsstivrcr scrayinpyv qfverqkwkc nicfrinplp
      601 ddfmydpstk kygeperrpe vrnatvefia pteymlrppq paaylfvldv shgavqtgyl
      661 ssfckvllee ldnipgdsrt qvgfvtfdss vhfynlgegl sqpqmmlvsd iddvfvpspd
      721 sllvnihesk alvqdlltnl plihaqngep tsalgaavqa gyklmspigg rvsvfqtilp
      781 nagpgslkpr edpnqragke ilnllpatdf ykklaldcss qqvavdvflm gaqymdvasl
      841 aciskysagc vsyypglhst lnrsqvekfe aelryyvtrk igfeavmrir ctkglvihtf
      901 hgnffvrstd llslpnvnpd agfgmqiaie esladcnvac fqaavlytss rgerrirvht
      961 lclpvtamlq dilgsvdqea vmgmlskmav drslsssvvd arealvnacl dliqafkttl
     1021 gsgqaaalgl piphcarlvp lyalalmkht afrvgistkl dervfameqm ktmpltylmt
     1081 yiypalyplh alddqqdale lddgtlvcqp prlqlsfeni drhgcyaldt vdrlylyvgr
     1141 alsdhfcmnv lgvpnflaip edmtelpqla teeservrnf lswtqsrrpf ysplrvired
     1201 skdrhlfvqh mvddrtdsal syyeflqhlk qqlsk
//
LOCUS       XP_077505314            1234 aa            linear   INV 25-NOV-2025
DEFINITION  protein transport protein Sec24AB isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505314
VERSION     XP_077505314.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649188.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1234
                     /product="protein transport protein Sec24AB isoform X2"
                     /calculated_mol_wt=132360
     Region          <17..441
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          383..1234
                     /region_name="COG5028"
                     /note="Vesicle coat complex COPII, subunit SEC24/subunit
                     SFB2/subunit SFB3 [Intracellular trafficking and
                     secretion]"
                     /db_xref="CDD:227361"
     CDS             1..1234
                     /gene="Sec24AB"
                     /coded_by="XM_077649188.1:14..3718"
                     /db_xref="GeneID:144115057"
ORIGIN      
        1 mqgmampggq gvpyggaipy grpgppasgv aegisphavp ygdsahhvak eaqllqapps
       61 agaaqlppmq skpngptvng swthlgggyt ngpslkvssh qqqvpssspa gvassrvnqg
      121 yqqpgssplv tptaseptsa kssrnaspip sqrydfmegq fssprpspis ymhpstsqaa
      181 ppvrgppppa gffpptpqgs nmppsarivp qpgppligqp pvsgtslqrp tpvsgpigpp
      241 pptsmsgsty pvsnpssafp gpptsgvhaa ssnqhlaagt qpsfqgqppl ptsfrpvsgp
      301 ppvpgphtlp ganrvpgppa avpptsvpaq pssqfpppss gvsgasfpgp rtaglagptm
      361 agpgmapasv sqpgthtqgf gpqppqgstl gapqqrpgyp gppptsgapa qlqtlhgrps
      421 ypqqfgysqs vgnssmpppp plynaqqqpm qqppleppig gapgydpelh srmaglsvna
      481 sfnklwgyea cnllqsrnil pkapedppkp slpgenancs tdifrctltk ipetqsllqk
      541 arlplgvlih pfrdvshlpv iqsstivrcr scrayinpyv qfverqkwkc nicfrinplp
      601 ddfmydpstk kygeperrpe vrnatvefia pteymlrppq paaylfvldv shgavqtgyl
      661 ssfckvllee ldnipgdsrt qvgfvtfdss vhfynlgegl sqpqmmlvsd iddvfvpspd
      721 sllvnihesk alvqdlltnl plihaqngep tsalgaavqa gyklmspigg rvsvfqtilp
      781 nagpgslkpr edpnqragke ilnllpatdf ykklaldcss qqvavdvflm gaqymdvasl
      841 aciskysagc vsyypglhst lnrsqvekfe aelryyvtrk igfeavmrir ctkglvihtf
      901 hgnffvrstd llslpnvnpd agfgmqiaie esladcnvac fqaavlytss rgerrirvht
      961 lclpvtamlq dilgsvdqea vmgmlskmav drslsssvvd arealvnacl dliqafkttl
     1021 gsgqaaalgl piphcarlvp lyalalmkht afrvgistkl dervfameqm ktmpltylmt
     1081 yiypalyplh alddqdalel ddgtlvcqpp rlqlsfenid rhgcyaldtv drlylyvgra
     1141 lsdhfcmnvl gvpnflaipe dmtelpqlat eeservrnfl swtqsrrpfy splrvireds
     1201 kdrhlfvqhm vddrtdsals yyeflqhlkq qlsk
//
LOCUS       XP_077505315            1204 aa            linear   INV 25-NOV-2025
DEFINITION  protein transport protein Sec24AB isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505315
VERSION     XP_077505315.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649189.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1204
                     /product="protein transport protein Sec24AB isoform X3"
                     /calculated_mol_wt=129021
     Region          <17..410
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          352..1204
                     /region_name="COG5028"
                     /note="Vesicle coat complex COPII, subunit SEC24/subunit
                     SFB2/subunit SFB3 [Intracellular trafficking and
                     secretion]"
                     /db_xref="CDD:227361"
     CDS             1..1204
                     /gene="Sec24AB"
                     /coded_by="XM_077649189.1:14..3628"
                     /db_xref="GeneID:144115057"
ORIGIN      
        1 mqgmampggq gvpyggaipy grpgppasgv aegisphavp ygdsahhvak eaqllqapps
       61 agaaqlppmq skpngptvng swthlgggyt ngpslkvssh qqqvpssspa gvassrvnqg
      121 yqqpgssplv tptasgpisy mhpstsqaap pvrgppppag ffpptpqgsn mppsarivpq
      181 pgppligqpp vsgtslqrpt pvsgpigppp ptsmsgstyp vsnpssafpg pptsgvhaas
      241 snqhlaagtq psfqgqpplp tsfrpvsgpp pvpgphtlpg anrvpgppaa vpptsvpaqp
      301 ssqfpppssg vsgasfpgpr taglagptma gpgmapasvs qpgthtqgfg pqppqgstlg
      361 apqqrpgypg ppptsgapaq lqtlhgrpsy pqqfgysqsv gnssmppppp lynaqqqpmq
      421 qppleppigg apgydpelhs rmaglsvnas fnklwgyeac nllqsrnilp kapedppkps
      481 lpgenancst difrctltki petqsllqka rlplgvlihp frdvshlpvi qsstivrcrs
      541 crayinpyvq fverqkwkcn icfrinplpd dfmydpstkk ygeperrpev rnatvefiap
      601 teymlrppqp aaylfvldvs hgavqtgyls sfckvlleel dnipgdsrtq vgfvtfdssv
      661 hfynlgegls qpqmmlvsdi ddvfvpspds llvniheska lvqdlltnlp lihaqngept
      721 salgaavqag yklmspiggr vsvfqtilpn agpgslkpre dpnqragkei lnllpatdfy
      781 kklaldcssq qvavdvflmg aqymdvasla ciskysagcv syypglhstl nrsqvekfea
      841 elryyvtrki gfeavmrirc tkglvihtfh gnffvrstdl lslpnvnpda gfgmqiaiee
      901 sladcnvacf qaavlytssr gerrirvhtl clpvtamlqd ilgsvdqeav mgmlskmavd
      961 rslsssvvda realvnacld liqafkttlg sgqaaalglp iphcarlvpl yalalmkhta
     1021 frvgistkld ervfameqmk tmpltylmty iypalyplha lddqqdalel ddgtlvcqpp
     1081 rlqlsfenid rhgcyaldtv drlylyvgra lsdhfcmnvl gvpnflaipe dmtelpqlat
     1141 eeservrnfl swtqsrrpfy splrvireds kdrhlfvqhm vddrtdsals yyeflqhlkq
     1201 qlsk
//
LOCUS       XP_077505316            1203 aa            linear   INV 25-NOV-2025
DEFINITION  protein transport protein Sec24AB isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505316
VERSION     XP_077505316.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649190.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1203
                     /product="protein transport protein Sec24AB isoform X4"
                     /calculated_mol_wt=128893
     Region          <17..410
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          352..1203
                     /region_name="COG5028"
                     /note="Vesicle coat complex COPII, subunit SEC24/subunit
                     SFB2/subunit SFB3 [Intracellular trafficking and
                     secretion]"
                     /db_xref="CDD:227361"
     CDS             1..1203
                     /gene="Sec24AB"
                     /coded_by="XM_077649190.1:14..3625"
                     /db_xref="GeneID:144115057"
ORIGIN      
        1 mqgmampggq gvpyggaipy grpgppasgv aegisphavp ygdsahhvak eaqllqapps
       61 agaaqlppmq skpngptvng swthlgggyt ngpslkvssh qqqvpssspa gvassrvnqg
      121 yqqpgssplv tptasgpisy mhpstsqaap pvrgppppag ffpptpqgsn mppsarivpq
      181 pgppligqpp vsgtslqrpt pvsgpigppp ptsmsgstyp vsnpssafpg pptsgvhaas
      241 snqhlaagtq psfqgqpplp tsfrpvsgpp pvpgphtlpg anrvpgppaa vpptsvpaqp
      301 ssqfpppssg vsgasfpgpr taglagptma gpgmapasvs qpgthtqgfg pqppqgstlg
      361 apqqrpgypg ppptsgapaq lqtlhgrpsy pqqfgysqsv gnssmppppp lynaqqqpmq
      421 qppleppigg apgydpelhs rmaglsvnas fnklwgyeac nllqsrnilp kapedppkps
      481 lpgenancst difrctltki petqsllqka rlplgvlihp frdvshlpvi qsstivrcrs
      541 crayinpyvq fverqkwkcn icfrinplpd dfmydpstkk ygeperrpev rnatvefiap
      601 teymlrppqp aaylfvldvs hgavqtgyls sfckvlleel dnipgdsrtq vgfvtfdssv
      661 hfynlgegls qpqmmlvsdi ddvfvpspds llvniheska lvqdlltnlp lihaqngept
      721 salgaavqag yklmspiggr vsvfqtilpn agpgslkpre dpnqragkei lnllpatdfy
      781 kklaldcssq qvavdvflmg aqymdvasla ciskysagcv syypglhstl nrsqvekfea
      841 elryyvtrki gfeavmrirc tkglvihtfh gnffvrstdl lslpnvnpda gfgmqiaiee
      901 sladcnvacf qaavlytssr gerrirvhtl clpvtamlqd ilgsvdqeav mgmlskmavd
      961 rslsssvvda realvnacld liqafkttlg sgqaaalglp iphcarlvpl yalalmkhta
     1021 frvgistkld ervfameqmk tmpltylmty iypalyplha lddqdaleld dgtlvcqppr
     1081 lqlsfenidr hgcyaldtvd rlylyvgral sdhfcmnvlg vpnflaiped mtelpqlate
     1141 eservrnfls wtqsrrpfys plrviredsk drhlfvqhmv ddrtdsalsy yeflqhlkqq
     1201 lsk
//
LOCUS       XP_077505317            1461 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: sushi, von Willebrand factor type A, EGF and
            pentraxin domain-containing protein 1-like [Amblyomma americanum].
ACCESSION   XP_077505317
VERSION     XP_077505317.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649191.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1461
                     /product="LOW QUALITY PROTEIN: sushi, von Willebrand
                     factor type A, EGF and pentraxin domain-containing protein
                     1-like"
                     /calculated_mol_wt=162930
     Region          122..176
                     /region_name="CCP"
                     /note="Complement control protein (CCP) modules (aka short
                     consensus repeats SCRs or SUSHI repeats) have been
                     identified in several proteins of the complement system;
                     cd00033"
                     /db_xref="CDD:153056"
     Site            order(132,149)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     Region          180..417
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     Region          <463..614
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     Region          719..781
                     /region_name="CCP"
                     /note="Domain abundant in complement control proteins;
                     SUSHI repeat; short complement-like repeat (SCR);
                     smart00032"
                     /db_xref="CDD:214478"
     Site            order(727,754)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     Region          <817..918
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1210..>1367
                     /region_name="PHA02639"
                     /note="EEV host range protein; Provisional"
                     /db_xref="CDD:165022"
     Region          1370..1427
                     /region_name="CCP"
                     /note="Complement control protein (CCP) modules (aka short
                     consensus repeats SCRs or SUSHI repeats) have been
                     identified in several proteins of the complement system;
                     cd00033"
                     /db_xref="CDD:153056"
     Site            order(1380,1400)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     CDS             1..1461
                     /gene="LOC144115058"
                     /coded_by="XM_077649191.1:1050..5435"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:144115058"
ORIGIN      
        1 maawrslhrv llllfvlwaa vdayhirtfg skgesrktsr ryaeswympr daptdleeet
       61 tdaegeeedv regcpqnree aaalgrrclr kcktdedcis skkkclcdgr cgwscvrpdl
      121 ncdeleplen gqfrvsgdyf garvyyecae nfwmsgpker mcqgdgrwsg rapeckkqps
      181 cssppgvphs rtnasdgard fvinstvryt cfpgydargf dvakcifyng saqwfgpdlk
      241 cepkscgppg evehgrrmgs mtrftssvky echegyelfg rshrycqssg qwsgtlpecr
      301 pvqcdkpedp lngraiydql lfnsvvryec hhgfrlkgps tarcnsqrhw egapthcvei
      361 dcghpghlhn gyvefrsstl nakatyncfd gmkfqgesnt sicldtgnws tplpkclapc
      421 kiptlehahv nlstpesrle hgrqlrvsch ehyelaynat pprcnngswt hlpicaparc
      481 kslperpahg fviapktdhg mralfhctdg yqlvgpnvtv cqfgnwthea vpvcreiycp
      541 fpgsiehgrv llvgnmgmyd yrpyvrrvsn nrqimfecrr gytilhgppg atcvdgrwsp
      601 selprcvrgs hphvrqirsa rrrrrrlaar trrdahtrrr rwrrtggpcr lapdaregps
      661 qrgrsdgatr saeptgvrrg relcgavnat arcvragwqp srerrlcrpr prrraptcnl
      721 pevphgsfre ltterpldpe elighgtlve lvcargfqvq gqpelrcweg ewtppgllpe
      781 clpepcelpa lrggrylggy ragltispgs sveyacdeph lrqwpasplr crmgrlqpap
      841 ptclppmptq ltpqtpsapg sgqgaaaeet sppksaapaa vvveesppta spappaggda
      901 vprratsppp sdrpcsaper lhnalafrss spsrseeeek eegeegdsrg llqfphgtev
      961 vfrcidgagg gerstwkllc edgswlgrpe kcedgaqqls vpkeerrnrs cvfrntepnl
     1021 eafqgdrrit ddalhfppgs elvfrckdig kyslvgsvrr rcvygdwdgv kpscfglsqe
     1081 ndyalekppt ilfrphvmpv aqsndgklvv ypgailhlec lwlrkygtpk wevshksrky
     1141 aegwttepgr dsqleyrlsi yhakkddsgr ytcitpmghk hsvdivvspv hcrplpsvsg
     1201 lsvstattrm gtkvvfscde gqrlrgsqqa mclpsgnwsa qppvcqvaec hdisgasdgl
     1261 vvvkaeqrav gsrarfscpl gyalrgqesv ecldtgqwsg slprceevvc aalpapesgy
     1321 vegerqpkyr ggqrmqfacr pdhmmlgsgv ldcldndrws gtvpkcvpac qypsvrdgar
     1381 issrvsyfyr inetvsfecp dgfqlrgspm iqcvakgrws aavprcqpfa pspplherrl
     1441 rrhrrrwpha tpvaearrwr l
//
LOCUS       XP_077505318             558 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115059 [Amblyomma americanum].
ACCESSION   XP_077505318
VERSION     XP_077505318.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649192.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..558
                     /product="uncharacterized protein LOC144115059"
                     /calculated_mol_wt=63048
     CDS             1..558
                     /gene="LOC144115059"
                     /coded_by="XM_077649192.1:83..1759"
                     /db_xref="GeneID:144115059"
ORIGIN      
        1 msdnenasic flwttynpcv enavfeymke eagskelyil rtetladdls sctvlgflvn
       61 alrkrnafvn rsttvlsvpe htqaiylcaa rvtlresakf etsipqvhna dvayrgrlsk
      121 sdvwqfepec vqeydeflsh tntvnswqil qmfrdaledd stfaaqfans vflvygpite
      181 fgkeciells qgrkirrivt eapldmsane vyniedvsvs sevadvpepd rsavenldsn
      241 tvaymvfitn afirvllnss delalatama spivelphdg ftrlkylsln ektpmcetai
      301 shvkranlrg dscampedrp lkryfcklen fvkllsdlqn tvevfpttna asviintlvl
      361 rinkaakrrg lvkqrvthfk deltrlarrf qdeegtgkvd dakdtvqgsa tpkilqrlsd
      421 ylstrriscp rqqlihnfgd ygtplnipem vkyfrtpvse dedddgydvp lherlakncq
      481 sdsereylsl lhaeaekknk rrsrrskqkw ehdlyippkv yavtdeapla elksagkkaa
      541 krslladisg tkkkasdn
//
LOCUS       XP_077505319             123 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115061 [Amblyomma americanum].
ACCESSION   XP_077505319
VERSION     XP_077505319.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649193.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..123
                     /product="uncharacterized protein LOC144115061"
                     /calculated_mol_wt=13496
     CDS             1..123
                     /gene="LOC144115061"
                     /coded_by="XM_077649193.1:255..626"
                     /db_xref="GeneID:144115061"
ORIGIN      
        1 maisvqpanf tilhvgdndl srsnanpqfi rdsikavvci ccatcppppp csaclrglai
       61 lprrfstada easrclldrc rlfnlkltat trtftswiqq krrrgnfllv tgttcrqake
      121 lks
//
LOCUS       XP_077505320             123 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115061 [Amblyomma americanum].
ACCESSION   XP_077505320
VERSION     XP_077505320.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649194.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..123
                     /product="uncharacterized protein LOC144115061"
                     /calculated_mol_wt=13496
     CDS             1..123
                     /gene="LOC144115061"
                     /coded_by="XM_077649194.1:256..627"
                     /db_xref="GeneID:144115061"
ORIGIN      
        1 maisvqpanf tilhvgdndl srsnanpqfi rdsikavvci ccatcppppp csaclrglai
       61 lprrfstada easrclldrc rlfnlkltat trtftswiqq krrrgnfllv tgttcrqake
      121 lks
//
LOCUS       XP_077505322             123 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115061 [Amblyomma americanum].
ACCESSION   XP_077505322
VERSION     XP_077505322.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649196.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..123
                     /product="uncharacterized protein LOC144115061"
                     /calculated_mol_wt=13496
     CDS             1..123
                     /gene="LOC144115061"
                     /coded_by="XM_077649196.1:257..628"
                     /db_xref="GeneID:144115061"
ORIGIN      
        1 maisvqpanf tilhvgdndl srsnanpqfi rdsikavvci ccatcppppp csaclrglai
       61 lprrfstada easrclldrc rlfnlkltat trtftswiqq krrrgnfllv tgttcrqake
      121 lks
//
LOCUS       XP_077505323            1195 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: SUN domain-containing ossification factor
            [Amblyomma americanum].
ACCESSION   XP_077505323
VERSION     XP_077505323.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649197.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1195
                     /product="LOW QUALITY PROTEIN: SUN domain-containing
                     ossification factor"
                     /calculated_mol_wt=129157
     Region          355..458
                     /region_name="Sad1_UNC"
                     /note="Sad1 / UNC-like C-terminal; pfam07738"
                     /db_xref="CDD:400199"
     Region          829..>946
                     /region_name="COG4372"
                     /note="Uncharacterized protein, contains DUF3084 domain
                     [Function unknown]"
                     /db_xref="CDD:443500"
     CDS             1..1195
                     /gene="LOC144115062"
                     /coded_by="XM_077649197.1:47..3634"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:144115062"
ORIGIN      
        1 mssassvfql qcslllsvac llalpravle plarwdaarw assaslpdwl trvrrpmgpg
       61 ctccckedve ndiadiartn gvkqashtaa atsdpevptq efpehskhlh svsphqsddl
      121 hvpppvevit qgvglssass gekqeeless lsasdiapal ddkprettea lesdqapvti
      181 ianqapdqtn dtehmeavtp epitetqrrt teesvfpgtk qdqepqgpdd tsvkepvegk
      241 edsfvpsppt edkvetrevp esalkkegde gtehdrkesa ekmptfvewk qkmlaehekg
      301 geqppevgss lpkkkptgsk srrnyasyec gakvlaanae adgagrvlne qmdeymlnpc
      361 kakiwfvvel cemiqvsqid ianfelfssm pkdfavlmsd ryptrdwtpl gtftahdrkv
      421 vqsfelhsea ygkyikvell srygsehycp lsllrvfgas middyeqlve kprdpsapdd
      481 deeetstegs asknvvdrak dvvisllkvl rrdqegegvd lmasapcsdl nancsilnvs
      541 hqvhnatsgq lgcqrnrsrh ffdsvfqqcs tcsrsrghst dtqtpsetia crflrtalsl
      601 edsctidkpd kapelyeste ssssnflvad sseftatsst vevavsssaq vrtmpsasav
      661 ndtseveask svampsseta qpestkafss pssvttvsss etsisvdlsh cdnettledf
      721 gdaselaekd stashtsads mtlqsssqvs dstadsatat pvivdvptsv ktspsstsva
      781 gievaspvmd salpndvttv sgvsppdvds ggvliigtld elvpdvdlql reagirappp
      841 eagalalssq resvfmrinn rikalelnms lsgqyleels rryrhqmdem qrafnrtvna
      901 lnktaqkaae kdlrqqetlv qlqqqldnlt evvntllaer htlskqvfgn hmclilieai
      961 ilaavfllcl rrvnapptll iqqesaprpl tpgrlvlkrr tasvgheeri eelatvaekk
     1021 qkssdegsdt knsfvivept mpivveaaps pkektkkvnk qkkhkgprrk tslpcypsck
     1081 adpggrvkes lipesnagvl fssrsaprnt ppvpkaatad tavvaaaaaa aakgigmgas
     1141 kipkgteskn gqngtlktna lrpadcirws gqsccekddh lglikflrlp gktpv
//
LOCUS       XP_077505324            2661 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115064 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505324
VERSION     XP_077505324.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649198.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2661
                     /product="uncharacterized protein LOC144115064 isoform X1"
                     /calculated_mol_wt=289954
     Region          <1455..1794
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <1703..>1795
                     /region_name="PRK00409"
                     /note="recombination and DNA strand exchange inhibitor
                     protein; Reviewed"
                     /db_xref="CDD:234750"
     CDS             1..2661
                     /gene="LOC144115064"
                     /coded_by="XM_077649198.1:165..8150"
                     /db_xref="GeneID:144115064"
ORIGIN      
        1 mttdpsgawl elrelhssln ylkdklnktg qklsaascsg piefnftslp sslasssncv
       61 plsrvatsas slinskrcrs gthttgkrna lngiplsqaq srerghipce lspiengall
      121 hhgevefmrs lrqpstrssf vpsgtaittt nnvtftidgi vtehslstgg lsvkkahyin
      181 delrkqsrss gskalvgssk klrrlvekqk kaaehrhssv kspqhqtdri ashvdahtsp
      241 dvtrgfsprl vdaatdcrph satfathtsa trgheqskkl leghdisrss vipdivenss
      301 lpsedcvegy ldylknrati graeeargep ntaenvlgng lqgyeypfes dclglawnea
      361 svdpvwkrkf asvrqpkqyr gfsltvdpst rrkphqptsk ggktegacel plapietavq
      421 ttettgecrs tcakvvekan avrptkpagi tttswkegrd lalkrhgpsd asvdaaaise
      481 ehprkktttt yaqarplpae vhreksiipn aarvaalrnl qrnrsvkrka lhtmaeqegn
      541 apvpatklrh cdspavndfi mkqklgrrqq krsserciae aaqarqqrle rlytfqrkta
      601 sesarigrek vrrqqedeql vhgfvehlks aqnefnqkdt phmfcpgsnl kvasanddld
      661 ekcgmqlvks qgvqvggvnt cnttdseisq dglscqsshs asctsghlqy fdgdifkpqq
      721 ptstdtnftm gtpvgcerpl gleqvfqyep telvvrltqm tsslneklde qlqmlgcdvp
      781 lqapefgehh psmvteriqr mvaafeiask pqpevhdcaf qhmrsskqss dkapdsqvha
      841 qvqhdaakki qhffrhcirn rkkalekghh gynrdggmkm lscileessl pqsskpsvig
      901 skpsvldeep syhlmpdpnt iarawkkksv kgaaaaaptp rkmsapptls eglqreneya
      961 sggtsaeglt slleedtgap sssfttshrn atvsvqssks egapekmlpd kpavlvvdsh
     1021 smarslskpk eaagkaggqd lqqhrhskss etslvpvrds kvaigafgvf grseplrasl
     1081 dsnrawdvle tqakalqasa etvrqlaqvr apslvelhge dmvqrltast vvaasalaaa
     1141 lasqrpdwqa pkdkcqrrdv yassssstsq ptdagfesnd ktspqssaee scspqtgsgk
     1201 qvletqlasr qdgsnasmdd sersgssgge siseeldtlg fsdssldrkk snsgtskkrq
     1261 tknvpprsle ssatedsagp tnlqvgrqss lsetpmldrd llsygslter pltsrpsgie
     1321 lveekeggdh gppgallrlq ehdiierara dftwievlkr ncrekgtedk lpalrkrera
     1381 vlirlhqkrm elkalqsqcl aqsleggsla sasnvtsipt hgatevaeas argvhssgis
     1441 epyeppllgn etsgqskess kpeevpskge qksslpnsyd stfessgtfp efedsthlkl
     1501 nasqrfleer qqklqsrrkh vqqllewqkk lnteeanvra lerralaklr argaktppqg
     1561 klessvttsv psqldaqtsk skgsgepksv typedasfee evseevdaka tsvtesiqee
     1621 vtrtestsgg hnqstieskv vtsvsseaev stqtegamkr ssngsrkgll likkplvvrr
     1681 kvrrdssgse dsfnvslset asdqsdiegr iialseelkk rqleaeqlkr eqrrrrteml
     1741 rereeslkkh ielydkliqq akeelekeld vaqhektafv kpqikkpraa eqrkhrlvhl
     1801 tvhlsgseng aqsalmlqnk dqkpcpgslv atpsasskks eseigteeeh tnegtsfass
     1861 saegltpkla tpsvsqkste kpsprvsals tssdiveels skevaepsss estevlhfga
     1921 atlsdglksa eescsqalda klnlegfrpi nteateaats qdntsvpaet tsepisnvaa
     1981 aaviksttdl tlrcspdgnl dsaassgedi sehisvtase sdsheqstkg klnaceqpvg
     2041 epyvlasdrs ecqndttsdg vfsslggiea slghevkels nrstdeglsd devqvlgktc
     2101 sdkvlaladd levasglkea sspagskstd ssgwpssqdd estvaeevae yteeivrsvv
     2161 ivsnqsceer ieegrvneav nsilrylmed aieslvrrrg pcvqhaekfl tgqeassiea
     2221 aivtqneynp sadvladneh ltvplkeavt taadisienk lvvevcsdet lpagvvqier
     2281 aeaaesfenl eqsdqpwdme qinaiadklv seaidsvlai akeegllsav svesgsqvsn
     2341 vsqkvnaila sieeprnsre fqrpqdlmvl stdlddeefs wkdasavpvh alsedapsea
     2401 gakalcsrav qaedcflstt teqdwfdddf glgsgdnalg yqrripnkpp ppysppkggf
     2461 msrlfseaaw kvpntesevs alvrkaaatv yttvmqgrsl aelafnqeyi dekapakafi
     2521 tdtnshhayc eflfdlvket arelfcteat dmppppwqrs vrlrrkqplp stlesfvhil
     2581 erkvmsvpgl henselafrn fdnrglsfvd mfvysearse epewvdysre evvvkdqvat
     2641 aifqllvddt levlkslwql r
//
LOCUS       XP_077505325            2660 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115064 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505325
VERSION     XP_077505325.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649199.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2660
                     /product="uncharacterized protein LOC144115064 isoform X2"
                     /calculated_mol_wt=289883
     Region          <1454..1793
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <1702..>1794
                     /region_name="PRK00409"
                     /note="recombination and DNA strand exchange inhibitor
                     protein; Reviewed"
                     /db_xref="CDD:234750"
     CDS             1..2660
                     /gene="LOC144115064"
                     /coded_by="XM_077649199.1:212..8194"
                     /db_xref="GeneID:144115064"
ORIGIN      
        1 mttdpsgawl elrelhssln ylkdklnktg qklsaascsg piefnftslp sslasssncv
       61 plsrvatsas slinskrcrs gthttgkrna lngiplsqaq srerghipce lspiengall
      121 hhgevefmrs lrqpstrssf vpsgtaittt nnvtftidgi vtehslstgg lsvkkahyin
      181 delrkqsrss gskalvgssk klrrlvekqk kaaehrhssv kspqhqtdri ashvdahtsp
      241 dvtrgfsprl vdaatdcrph satfathtsa trgheqskkl leghdisrss vipdivenss
      301 lpsedcvegy ldylknrati graeeargep ntaenvlgng lqgyeypfes dclglawnea
      361 svdpvwkrkf asvrqpkqyr gfsltvdpst rrkphqptsk ggktegacel plapietavq
      421 ttettgecrs tcakvvekan avrptkpagi tttswkegrd lalkrhgpsd asvdaaaise
      481 ehprkktttt yaqarplpae vhreksiipn aarvaalrnl qrnrsvkrka lhtmeqegna
      541 pvpatklrhc dspavndfim kqklgrrqqk rsserciaea aqarqqrler lytfqrktas
      601 esarigrekv rrqqedeqlv hgfvehlksa qnefnqkdtp hmfcpgsnlk vasanddlde
      661 kcgmqlvksq gvqvggvntc nttdseisqd glscqsshsa sctsghlqyf dgdifkpqqp
      721 tstdtnftmg tpvgcerplg leqvfqyept elvvrltqmt sslnekldeq lqmlgcdvpl
      781 qapefgehhp smvteriqrm vaafeiaskp qpevhdcafq hmrsskqssd kapdsqvhaq
      841 vqhdaakkiq hffrhcirnr kkalekghhg ynrdggmkml scileesslp qsskpsvigs
      901 kpsvldeeps yhlmpdpnti arawkkksvk gaaaaaptpr kmsapptlse glqreneyas
      961 ggtsaeglts lleedtgaps ssfttshrna tvsvqsskse gapekmlpdk pavlvvdshs
     1021 marslskpke aagkaggqdl qqhrhsksse tslvpvrdsk vaigafgvfg rseplrasld
     1081 snrawdvlet qakalqasae tvrqlaqvra pslvelhged mvqrltastv vaasalaaal
     1141 asqrpdwqap kdkcqrrdvy assssstsqp tdagfesndk tspqssaees cspqtgsgkq
     1201 vletqlasrq dgsnasmdds ersgssgges iseeldtlgf sdssldrkks nsgtskkrqt
     1261 knvpprsles satedsagpt nlqvgrqssl setpmldrdl lsygslterp ltsrpsgiel
     1321 veekeggdhg ppgallrlqe hdiierarad ftwievlkrn crekgtedkl palrkrerav
     1381 lirlhqkrme lkalqsqcla qsleggslas asnvtsipth gatevaeasa rgvhssgise
     1441 pyeppllgne tsgqskessk peevpskgeq ksslpnsyds tfessgtfpe fedsthlkln
     1501 asqrfleerq qklqsrrkhv qqllewqkkl nteeanvral erralaklra rgaktppqgk
     1561 lessvttsvp sqldaqtsks kgsgepksvt ypedasfeee vseevdakat svtesiqeev
     1621 trtestsggh nqstieskvv tsvsseaevs tqtegamkrs sngsrkglll ikkplvvrrk
     1681 vrrdssgsed sfnvslseta sdqsdiegri ialseelkkr qleaeqlkre qrrrrtemlr
     1741 ereeslkkhi elydkliqqa keelekeldv aqhektafvk pqikkpraae qrkhrlvhlt
     1801 vhlsgsenga qsalmlqnkd qkpcpgslva tpsasskkse seigteeeht negtsfasss
     1861 aegltpklat psvsqkstek psprvsalst ssdiveelss kevaepssse stevlhfgaa
     1921 tlsdglksae escsqaldak lnlegfrpin teateaatsq dntsvpaett sepisnvaaa
     1981 aviksttdlt lrcspdgnld saassgedis ehisvtases dsheqstkgk lnaceqpvge
     2041 pyvlasdrse cqndttsdgv fsslggieas lghevkelsn rstdeglsdd evqvlgktcs
     2101 dkvlaladdl evasglkeas spagskstds sgwpssqdde stvaeevaey teeivrsvvi
     2161 vsnqsceeri eegrvneavn silrylmeda ieslvrrrgp cvqhaekflt gqeassieaa
     2221 ivtqneynps advladnehl tvplkeavtt aadisienkl vvevcsdetl pagvvqiera
     2281 eaaesfenle qsdqpwdmeq inaiadklvs eaidsvlaia keegllsavs vesgsqvsnv
     2341 sqkvnailas ieeprnsref qrpqdlmvls tdlddeefsw kdasavpvha lsedapseag
     2401 akalcsravq aedcflsttt eqdwfdddfg lgsgdnalgy qrripnkppp pysppkggfm
     2461 srlfseaawk vpntesevsa lvrkaaatvy ttvmqgrsla elafnqeyid ekapakafit
     2521 dtnshhayce flfdlvketa relfcteatd mppppwqrsv rlrrkqplps tlesfvhile
     2581 rkvmsvpglh enselafrnf dnrglsfvdm fvysearsee pewvdysree vvvkdqvata
     2641 ifqllvddtl evlkslwqlr
//
LOCUS       XP_077505326            2660 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115064 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505326
VERSION     XP_077505326.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649200.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2660
                     /product="uncharacterized protein LOC144115064 isoform X3"
                     /calculated_mol_wt=289825
     Region          <1454..1793
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <1702..>1794
                     /region_name="PRK00409"
                     /note="recombination and DNA strand exchange inhibitor
                     protein; Reviewed"
                     /db_xref="CDD:234750"
     CDS             1..2660
                     /gene="LOC144115064"
                     /coded_by="XM_077649200.1:165..8147"
                     /db_xref="GeneID:144115064"
ORIGIN      
        1 mttdpsgawl elrelhssln ylkdklnktg qklsaascsg piefnftslp sslasssncv
       61 plsrvatsas slinskrcrs gthttgkrna lngiplsqaq srerghipce lspiengall
      121 hhgevefmrs lrqpstrssf vpsgtaittt nnvtftidgi vtehslstgg lsvkkahyin
      181 delrkqsrss gskalvgssk klrrlvekqk kaaehrhssv kspqhqtdri ashvdahtsp
      241 dvtrgfsprl vdaatdcrph satfathtsa trgheqskkl leghdisrss vipdivenss
      301 lpsedcvegy ldylknrati graeeargep ntaenvlgng lqgyeypfes dclglawnea
      361 svdpvwkrkf asvrqpkqyr gfsltvdpst rrkphqptsk ggktegacel plapietavq
      421 ttettgecrs tcakvvekan avrptkpagi tttswkegrd lalkrhgpsd asvdaaaise
      481 hprkktttty aqarplpaev hreksiipna arvaalrnlq rnrsvkrkal htmaeqegna
      541 pvpatklrhc dspavndfim kqklgrrqqk rsserciaea aqarqqrler lytfqrktas
      601 esarigrekv rrqqedeqlv hgfvehlksa qnefnqkdtp hmfcpgsnlk vasanddlde
      661 kcgmqlvksq gvqvggvntc nttdseisqd glscqsshsa sctsghlqyf dgdifkpqqp
      721 tstdtnftmg tpvgcerplg leqvfqyept elvvrltqmt sslnekldeq lqmlgcdvpl
      781 qapefgehhp smvteriqrm vaafeiaskp qpevhdcafq hmrsskqssd kapdsqvhaq
      841 vqhdaakkiq hffrhcirnr kkalekghhg ynrdggmkml scileesslp qsskpsvigs
      901 kpsvldeeps yhlmpdpnti arawkkksvk gaaaaaptpr kmsapptlse glqreneyas
      961 ggtsaeglts lleedtgaps ssfttshrna tvsvqsskse gapekmlpdk pavlvvdshs
     1021 marslskpke aagkaggqdl qqhrhsksse tslvpvrdsk vaigafgvfg rseplrasld
     1081 snrawdvlet qakalqasae tvrqlaqvra pslvelhged mvqrltastv vaasalaaal
     1141 asqrpdwqap kdkcqrrdvy assssstsqp tdagfesndk tspqssaees cspqtgsgkq
     1201 vletqlasrq dgsnasmdds ersgssgges iseeldtlgf sdssldrkks nsgtskkrqt
     1261 knvpprsles satedsagpt nlqvgrqssl setpmldrdl lsygslterp ltsrpsgiel
     1321 veekeggdhg ppgallrlqe hdiierarad ftwievlkrn crekgtedkl palrkrerav
     1381 lirlhqkrme lkalqsqcla qsleggslas asnvtsipth gatevaeasa rgvhssgise
     1441 pyeppllgne tsgqskessk peevpskgeq ksslpnsyds tfessgtfpe fedsthlkln
     1501 asqrfleerq qklqsrrkhv qqllewqkkl nteeanvral erralaklra rgaktppqgk
     1561 lessvttsvp sqldaqtsks kgsgepksvt ypedasfeee vseevdakat svtesiqeev
     1621 trtestsggh nqstieskvv tsvsseaevs tqtegamkrs sngsrkglll ikkplvvrrk
     1681 vrrdssgsed sfnvslseta sdqsdiegri ialseelkkr qleaeqlkre qrrrrtemlr
     1741 ereeslkkhi elydkliqqa keelekeldv aqhektafvk pqikkpraae qrkhrlvhlt
     1801 vhlsgsenga qsalmlqnkd qkpcpgslva tpsasskkse seigteeeht negtsfasss
     1861 aegltpklat psvsqkstek psprvsalst ssdiveelss kevaepssse stevlhfgaa
     1921 tlsdglksae escsqaldak lnlegfrpin teateaatsq dntsvpaett sepisnvaaa
     1981 aviksttdlt lrcspdgnld saassgedis ehisvtases dsheqstkgk lnaceqpvge
     2041 pyvlasdrse cqndttsdgv fsslggieas lghevkelsn rstdeglsdd evqvlgktcs
     2101 dkvlaladdl evasglkeas spagskstds sgwpssqdde stvaeevaey teeivrsvvi
     2161 vsnqsceeri eegrvneavn silrylmeda ieslvrrrgp cvqhaekflt gqeassieaa
     2221 ivtqneynps advladnehl tvplkeavtt aadisienkl vvevcsdetl pagvvqiera
     2281 eaaesfenle qsdqpwdmeq inaiadklvs eaidsvlaia keegllsavs vesgsqvsnv
     2341 sqkvnailas ieeprnsref qrpqdlmvls tdlddeefsw kdasavpvha lsedapseag
     2401 akalcsravq aedcflsttt eqdwfdddfg lgsgdnalgy qrripnkppp pysppkggfm
     2461 srlfseaawk vpntesevsa lvrkaaatvy ttvmqgrsla elafnqeyid ekapakafit
     2521 dtnshhayce flfdlvketa relfcteatd mppppwqrsv rlrrkqplps tlesfvhile
     2581 rkvmsvpglh enselafrnf dnrglsfvdm fvysearsee pewvdysree vvvkdqvata
     2641 ifqllvddtl evlkslwqlr
//
LOCUS       XP_077505327            2659 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115064 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505327
VERSION     XP_077505327.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649201.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2659
                     /product="uncharacterized protein LOC144115064 isoform X4"
                     /calculated_mol_wt=289754
     Region          <1453..1792
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <1701..>1793
                     /region_name="PRK00409"
                     /note="recombination and DNA strand exchange inhibitor
                     protein; Reviewed"
                     /db_xref="CDD:234750"
     CDS             1..2659
                     /gene="LOC144115064"
                     /coded_by="XM_077649201.1:165..8144"
                     /db_xref="GeneID:144115064"
ORIGIN      
        1 mttdpsgawl elrelhssln ylkdklnktg qklsaascsg piefnftslp sslasssncv
       61 plsrvatsas slinskrcrs gthttgkrna lngiplsqaq srerghipce lspiengall
      121 hhgevefmrs lrqpstrssf vpsgtaittt nnvtftidgi vtehslstgg lsvkkahyin
      181 delrkqsrss gskalvgssk klrrlvekqk kaaehrhssv kspqhqtdri ashvdahtsp
      241 dvtrgfsprl vdaatdcrph satfathtsa trgheqskkl leghdisrss vipdivenss
      301 lpsedcvegy ldylknrati graeeargep ntaenvlgng lqgyeypfes dclglawnea
      361 svdpvwkrkf asvrqpkqyr gfsltvdpst rrkphqptsk ggktegacel plapietavq
      421 ttettgecrs tcakvvekan avrptkpagi tttswkegrd lalkrhgpsd asvdaaaise
      481 hprkktttty aqarplpaev hreksiipna arvaalrnlq rnrsvkrkal htmeqegnap
      541 vpatklrhcd spavndfimk qklgrrqqkr sserciaeaa qarqqrlerl ytfqrktase
      601 sarigrekvr rqqedeqlvh gfvehlksaq nefnqkdtph mfcpgsnlkv asanddldek
      661 cgmqlvksqg vqvggvntcn ttdseisqdg lscqsshsas ctsghlqyfd gdifkpqqpt
      721 stdtnftmgt pvgcerplgl eqvfqyepte lvvrltqmts slnekldeql qmlgcdvplq
      781 apefgehhps mvteriqrmv aafeiaskpq pevhdcafqh mrsskqssdk apdsqvhaqv
      841 qhdaakkiqh ffrhcirnrk kalekghhgy nrdggmkmls cileesslpq sskpsvigsk
      901 psvldeepsy hlmpdpntia rawkkksvkg aaaaaptprk msapptlseg lqreneyasg
      961 gtsaegltsl leedtgapss sfttshrnat vsvqsskseg apekmlpdkp avlvvdshsm
     1021 arslskpkea agkaggqdlq qhrhsksset slvpvrdskv aigafgvfgr seplraslds
     1081 nrawdvletq akalqasaet vrqlaqvrap slvelhgedm vqrltastvv aasalaaala
     1141 sqrpdwqapk dkcqrrdvya ssssstsqpt dagfesndkt spqssaeesc spqtgsgkqv
     1201 letqlasrqd gsnasmddse rsgssggesi seeldtlgfs dssldrkksn sgtskkrqtk
     1261 nvpprsless atedsagptn lqvgrqssls etpmldrdll sygslterpl tsrpsgielv
     1321 eekeggdhgp pgallrlqeh diieraradf twievlkrnc rekgtedklp alrkreravl
     1381 irlhqkrmel kalqsqclaq sleggslasa snvtsipthg atevaeasar gvhssgisep
     1441 yeppllgnet sgqskesskp eevpskgeqk sslpnsydst fessgtfpef edsthlklna
     1501 sqrfleerqq klqsrrkhvq qllewqkkln teeanvrale rralaklrar gaktppqgkl
     1561 essvttsvps qldaqtsksk gsgepksvty pedasfeeev seevdakats vtesiqeevt
     1621 rtestsgghn qstieskvvt svsseaevst qtegamkrss ngsrkgllli kkplvvrrkv
     1681 rrdssgseds fnvslsetas dqsdiegrii alseelkkrq leaeqlkreq rrrrtemlre
     1741 reeslkkhie lydkliqqak eelekeldva qhektafvkp qikkpraaeq rkhrlvhltv
     1801 hlsgsengaq salmlqnkdq kpcpgslvat psasskkses eigteeehtn egtsfasssa
     1861 egltpklatp svsqkstekp sprvsalsts sdiveelssk evaepssses tevlhfgaat
     1921 lsdglksaee scsqaldakl nlegfrpint eateaatsqd ntsvpaetts episnvaaaa
     1981 viksttdltl rcspdgnlds aassgedise hisvtasesd sheqstkgkl naceqpvgep
     2041 yvlasdrsec qndttsdgvf sslggieasl ghevkelsnr stdeglsdde vqvlgktcsd
     2101 kvlaladdle vasglkeass pagskstdss gwpssqddes tvaeevaeyt eeivrsvviv
     2161 snqsceerie egrvneavns ilrylmedai eslvrrrgpc vqhaekfltg qeassieaai
     2221 vtqneynpsa dvladnehlt vplkeavtta adisienklv vevcsdetlp agvvqierae
     2281 aaesfenleq sdqpwdmeqi naiadklvse aidsvlaiak eegllsavsv esgsqvsnvs
     2341 qkvnailasi eeprnsrefq rpqdlmvlst dlddeefswk dasavpvhal sedapseaga
     2401 kalcsravqa edcflsttte qdwfdddfgl gsgdnalgyq rripnkpppp ysppkggfms
     2461 rlfseaawkv pntesevsal vrkaaatvyt tvmqgrslae lafnqeyide kapakafitd
     2521 tnshhaycef lfdlvketar elfcteatdm ppppwqrsvr lrrkqplpst lesfvhiler
     2581 kvmsvpglhe nselafrnfd nrglsfvdmf vysearseep ewvdysreev vvkdqvatai
     2641 fqllvddtle vlkslwqlr
//
LOCUS       XP_077505328             328 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin carboxy-terminal hydrolase L5 [Amblyomma americanum].
ACCESSION   XP_077505328
VERSION     XP_077505328.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649202.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..328
                     /product="ubiquitin carboxy-terminal hydrolase L5"
                     /calculated_mol_wt=37472
     Region          11..227
                     /region_name="Peptidase_C12_UCH37_BAP1"
                     /note="Cysteine peptidase C12 containing ubiquitin
                     carboxyl-terminal hydrolase (UCH) families UCH37 (UCH-L5)
                     and BAP1; cd09617"
                     /db_xref="CDD:187738"
     Site            order(85,91,167,182)
                     /site_type="other"
                     /note="cancer-causing mutation sites"
                     /db_xref="CDD:187738"
     Site            order(85,91,167,182)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:187738"
     Region          260..305
                     /region_name="UCH_C"
                     /note="Ubiquitin carboxyl-terminal hydrolases; pfam18031"
                     /db_xref="CDD:465622"
     CDS             1..328
                     /gene="Uch-L5"
                     /coded_by="XM_077649202.1:71..1057"
                     /db_xref="GeneID:144115065"
ORIGIN      
        1 maassgsagd wcliesdpgv ftelikgfgv kgvqveelws ldresfdklk pvhgliflfk
       61 wyrndepsgs ivqdnrleki ffakqvinna catqailsvl lnvkhpditl getlisfkdf
      121 cqsfdatmkg ltlsnsdvir evhnsfsrqq mfefdikqcs kdddvyhfig yvpidgrlye
      181 ldglkegpid lgpvpqgtdw idtvrpvieg rikqynegei hfnlmalitd rkllyqkqld
      241 slnaqlscgg metdeiqsei sklhmliqee enksrrykve nirrkhnylp limeilkils
      301 eekklvplve karekalekk kqgekmkq
//
LOCUS       XP_077505329             358 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115066 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505329
VERSION     XP_077505329.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649203.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..358
                     /product="uncharacterized protein LOC144115066 isoform X1"
                     /calculated_mol_wt=39570
     CDS             1..358
                     /gene="LOC144115066"
                     /coded_by="XM_077649203.1:122..1198"
                     /db_xref="GeneID:144115066"
ORIGIN      
        1 mvvctvdmae drircdycdh spsshsriad lepsnstecv hgnldarsce nllsqgaqah
       61 gghgpimqdt eqqnnasssv lsssqpgcck dslpwtgeec pvqcedtteg rneayssyqa
      121 pvqdahgmtp tqavanqcte slrttkclht stiasakdav apakkyipvv eksnpevtke
      181 maiaklqlfv sdtlssvtyn tervmcrlkv tfqhrcqhyv hplpsyflve dilcaeaqlr
      241 fgstvdelev rlkeaaqvfs ekqgqdtslv eisrhlqdsm dhflitetal ptaiqvtaph
      301 vytdknqswi yggtqaevia lkdkniewav iiclrvyylk ilsyprafsq tlilckns
//
LOCUS       XP_077505330             326 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115066 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505330
VERSION     XP_077505330.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649204.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..326
                     /product="uncharacterized protein LOC144115066 isoform X2"
                     /calculated_mol_wt=36268
     CDS             1..326
                     /gene="LOC144115066"
                     /coded_by="XM_077649204.1:122..1102"
                     /db_xref="GeneID:144115066"
ORIGIN      
        1 mvvctvdmae drircdycdh spsshsriad lgpimqdteq qnnasssvls ssqpgcckds
       61 lpwtgeecpv qcedttegrn eayssyqapv qdahgmtptq avanqctesl rttkclhtst
      121 iasakdavap akkyipvvek snpevtkema iaklqlfvsd tlssvtynte rvmcrlkvtf
      181 qhrcqhyvhp lpsyflvedi lcaeaqlrfg stvdelevrl keaaqvfsek qgqdtslvei
      241 srhlqdsmdh flitetalpt aiqvtaphvy tdknqswiyg gtqaevialk dkniewavii
      301 clrvyylkil syprafsqtl ilckns
//
LOCUS       XP_077505331             317 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115066 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505331
VERSION     XP_077505331.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649205.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..317
                     /product="uncharacterized protein LOC144115066 isoform X3"
                     /calculated_mol_wt=35206
     CDS             1..317
                     /gene="LOC144115066"
                     /coded_by="XM_077649205.1:122..1075"
                     /db_xref="GeneID:144115066"
ORIGIN      
        1 mvvctvdmae drircdycdh spsshsriad lepsnstecv hgnldarsce nllsqgaqah
       61 gghgpimqdt eqqnnasssv lsssqpgcck dslpwtgeec pvqcedtteg rneayssyqa
      121 pvqdahgmtp tqavanqcte slrttklfvs dtlssvtynt ervmcrlkvt fqhrcqhyvh
      181 plpsyflved ilcaeaqlrf gstvdelevr lkeaaqvfse kqgqdtslve isrhlqdsmd
      241 hflitetalp taiqvtaphv ytdknqswiy ggtqaevial kdkniewavi iclrvyylki
      301 lsyprafsqt lilckns
//
LOCUS       XP_077505332             292 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115066 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505332
VERSION     XP_077505332.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649206.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..292
                     /product="uncharacterized protein LOC144115066 isoform X4"
                     /calculated_mol_wt=32493
     CDS             1..292
                     /gene="LOC144115066"
                     /coded_by="XM_077649206.1:192..1070"
                     /db_xref="GeneID:144115066"
ORIGIN      
        1 mqdteqqnna sssvlsssqp gcckdslpwt geecpvqced ttegrneays syqapvqdah
       61 gmtptqavan qcteslrttk clhtstiasa kdavapakky ipvveksnpe vtkemaiakl
      121 qlfvsdtlss vtyntervmc rlkvtfqhrc qhyvhplpsy flvedilcae aqlrfgstvd
      181 elevrlkeaa qvfsekqgqd tslveisrhl qdsmdhflit etalptaiqv taphvytdkn
      241 qswiyggtqa evialkdkni ewaviiclrv yylkilsypr afsqtlilck ns
//
LOCUS       XP_077505333             849 aa            linear   INV 25-NOV-2025
DEFINITION  breast cancer anti-estrogen resistance protein 3 homolog isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077505333
VERSION     XP_077505333.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649207.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..849
                     /product="breast cancer anti-estrogen resistance protein 3
                     homolog isoform X1"
                     /calculated_mol_wt=93320
     Region          35..87
                     /region_name="SAM"
                     /note="Sterile alpha motif; smart00454"
                     /db_xref="CDD:197735"
     Region          189..323
                     /region_name="SH2_BCAR3"
                     /note="Src homology 2 (SH2) domain in the Breast Cancer
                     Anti-estrogen Resistance protein 3; cd10337"
                     /db_xref="CDD:198200"
     Site            order(203,219,240,242)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198200"
     Site            order(241,276)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198200"
     Region          564..>683
                     /region_name="RasGEF"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     small GTPases. Small GTP-binding proteins of the Ras
                     superfamily function as molecular switches in fundamental
                     events such as signal transduction, cytoskeleton dynamics
                     and intracellular trafficking; cl02485"
                     /db_xref="CDD:470590"
     CDS             1..849
                     /gene="LOC144115067"
                     /coded_by="XM_077649207.1:330..2879"
                     /db_xref="GeneID:144115067"
ORIGIN      
        1 mgnkhggclr spdirfdpvm svdpggkphl didvwlqnld lpqysllfck ytgveeilwm
       61 serdvkdlgv kngahrailv sslivlkrky dkgnrvksgg tlssrfrsss slvqakekpp
      121 nlggktlsyp nvlvhsavsp vsplslvrsc ststaasshr ssmetpvtte tpaedlkkal
      181 ewelgldsrd lrshawyhgt iprqraeelm tadgfflvrd cvsrpgdyvl tccwkgaplh
      241 fvinkvvlqp ftvyervqyq feddcfdtvp dlvtfyvgnk rpisaasgav vsrpvnrsmp
      301 lsyyaqrygi egpkavessq qrpldtssvv papsngitse phlpqkmhss shastgpmss
      361 catlpsrdss snhrrgfirv gsdpllsptl erralehrpl sgtvsseatq rnlegssedq
      421 pppkpsrvps krlqqkpvvv krdpvpvtqy vnncegvyse ladnggssim aetsdlavrp
      481 gdikrhamph rtfiragavs ttqaasaqcs davacnrvvt vpeidppssf dlvtfattll
      541 psdnkpldks imarvrnvll esgpralanh ltrldldvlg lkgerdlglg vtvglellal
      601 pqgsrfrkdl lerteciklf vastllmcad dvervellnk wiqvagdtkt algnlygftg
      661 imqglamsqv srlkttwlal rqrhtdtala yetklrpalk nmqecsnpqa pntclpylls
      721 lllilehnfe gllanlasap aqrkdsssel lllpwehsta dyglqlllsh leqqrlatqq
      781 iptfrrngei vfenvklddi mldlfrtefh llflwgarga tvascerhak ltqvlaamsv
      841 rcepigtpv
//
LOCUS       XP_077505334             687 aa            linear   INV 25-NOV-2025
DEFINITION  breast cancer anti-estrogen resistance protein 3 homolog isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077505334
VERSION     XP_077505334.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649208.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..687
                     /product="breast cancer anti-estrogen resistance protein 3
                     homolog isoform X2"
                     /calculated_mol_wt=75719
     Region          27..161
                     /region_name="SH2_BCAR3"
                     /note="Src homology 2 (SH2) domain in the Breast Cancer
                     Anti-estrogen Resistance protein 3; cd10337"
                     /db_xref="CDD:198200"
     Site            order(41,57,78,80)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198200"
     Site            order(79,114)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198200"
     Region          402..>521
                     /region_name="RasGEF"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     small GTPases. Small GTP-binding proteins of the Ras
                     superfamily function as molecular switches in fundamental
                     events such as signal transduction, cytoskeleton dynamics
                     and intracellular trafficking; cl02485"
                     /db_xref="CDD:470590"
     CDS             1..687
                     /gene="LOC144115067"
                     /coded_by="XM_077649208.1:577..2640"
                     /db_xref="GeneID:144115067"
ORIGIN      
        1 metpvttetp aedlkkalew elgldsrdlr shawyhgtip rqraeelmta dgfflvrdcv
       61 srpgdyvltc cwkgaplhfv inkvvlqpft vyervqyqfe ddcfdtvpdl vtfyvgnkrp
      121 isaasgavvs rpvnrsmpls yyaqrygieg pkavessqqr pldtssvvpa psngitseph
      181 lpqkmhsssh astgpmssca tlpsrdsssn hrrgfirvgs dpllsptler ralehrplsg
      241 tvsseatqrn legssedqpp pkpsrvpskr lqqkpvvvkr dpvpvtqyvn ncegvysela
      301 dnggssimae tsdlavrpgd ikrhamphrt firagavstt qaasaqcsda vacnrvvtvp
      361 eidppssfdl vtfattllps dnkpldksim arvrnvlles gpralanhlt rldldvlglk
      421 gerdlglgvt vglellalpq gsrfrkdlle rteciklfva stllmcaddv ervellnkwi
      481 qvagdtktal gnlygftgim qglamsqvsr lkttwlalrq rhtdtalaye tklrpalknm
      541 qecsnpqapn tclpyllsll lilehnfegl lanlasapaq rkdssselll lpwehstady
      601 glqlllshle qqrlatqqip tfrrngeivf envklddiml dlfrtefhll flwgargatv
      661 ascerhaklt qvlaamsvrc epigtpv
//
LOCUS       XP_077505335             319 aa            linear   INV 25-NOV-2025
DEFINITION  leucine-rich repeat transmembrane protein FLRT3-like [Amblyomma
            americanum].
ACCESSION   XP_077505335
VERSION     XP_077505335.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649209.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..319
                     /product="leucine-rich repeat transmembrane protein
                     FLRT3-like"
                     /calculated_mol_wt=35447
     Region          <130..>271
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          136..159
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          160..183
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          184..208
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          209..232
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          233..260
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          261..272
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     CDS             1..319
                     /gene="LOC144115068"
                     /coded_by="XM_077649209.1:120..1079"
                     /db_xref="GeneID:144115068"
ORIGIN      
        1 mlalrrlvlw allarlatat ptcvetgsph tryicsrfts hadfdrflsr dfppdrysrv
       61 dfvlrdsnld flpvaafegt rassvkfsnv rvhgetppsd sghpfsgvts wlkkvtfsdg
      121 stvphswsll aplskledlv ldnmhyvnls hgfnqlpksl krvrvtnsti sgvdsdwlqs
      181 lenieallve nsnlntfert mlprpapkle sltlrnanlt slpadltada phltvlnlqq
      241 nnithfnhds faplltqhpn vtahlegnpl ecdcharfln qipdnwttpp cnsperlrgr
      301 yvknigisql icdpasgah
//
LOCUS       XP_077505336             482 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger protein no ocelli isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505336
VERSION     XP_077505336.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649210.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..482
                     /product="zinc finger protein no ocelli isoform X1"
                     /calculated_mol_wt=48796
     Region          19..>44
                     /region_name="SP1-4_N"
                     /note="N-terminal domain of transcription factor
                     Specificity Proteins (SP) 1-4; cl41773"
                     /db_xref="CDD:425404"
     Region          345..371
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          352..371
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     CDS             1..482
                     /gene="noc"
                     /coded_by="XM_077649210.1:287..1735"
                     /db_xref="GeneID:144115069"
ORIGIN      
        1 mltssnqylr peflsplptt ldakksplal laqtcsniga dnpnikpiip plekpkdqdk
       61 skgkssslss sstssssqld dkpsfkpyes akkedsapng dvgggkksps ssskspprva
      121 ssspaasias sgkasptase hsksdassgs ataittttss ssstpssqnt lsglgyggaa
      181 algldlardk dalsqltsls aaykglnpla nccspllagh lpgavdggaf pgaalasqal
      241 klggypvgsp lspyvgyarv ktagggtslv pvcrdpfctn cqmsmqsaql gvcpsgctqc
      301 shdrlapagl glnpaalvpp glgsgglgsp fypgahsmlt rpnvcswmvg dtycgkrfst
      361 seellqhlrt htnlsvtdss alpllspsls vpsatsglsa aaaachlhys tapslttpag
      421 lrrtyptsls pvgslsaryh pykpplpafp gtpfaplphp slgvyyspyg lygqrlgppv
      481 hp
//
LOCUS       XP_077505337             467 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger protein no ocelli isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505337
VERSION     XP_077505337.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649211.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..467
                     /product="zinc finger protein no ocelli isoform X2"
                     /calculated_mol_wt=47049
     Region          7..>29
                     /region_name="SP1-4_N"
                     /note="N-terminal domain of transcription factor
                     Specificity Proteins (SP) 1-4; cl41773"
                     /db_xref="CDD:425404"
     Region          330..356
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          337..356
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     CDS             1..467
                     /gene="noc"
                     /coded_by="XM_077649211.1:241..1644"
                     /db_xref="GeneID:144115069"
ORIGIN      
        1 masiqldakk splallaqtc snigadnpni kpiipplekp kdqdkskgks sslsssstss
       61 ssqlddkpsf kpyesakked sapngdvggg kkspssssks pprvassspa asiassgkas
      121 ptasehsksd assgsatait tttssssstp ssqntlsglg yggaaalgld lardkdalsq
      181 ltslsaaykg lnplanccsp llaghlpgav dggafpgaal asqalklggy pvgsplspyv
      241 gyarvktagg gtslvpvcrd pfctncqmsm qsaqlgvcps gctqcshdrl apaglglnpa
      301 alvppglgsg glgspfypga hsmltrpnvc swmvgdtycg krfstseell qhlrthtnls
      361 vtdssalpll spslsvpsat sglsaaaaac hlhystapsl ttpaglrrty ptslspvgsl
      421 saryhpykpp lpafpgtpfa plphpslgvy yspyglygqr lgppvhp
//
LOCUS       XP_077505338            1117 aa            linear   INV 25-NOV-2025
DEFINITION  cohesin release factor wings apart-like [Amblyomma americanum].
ACCESSION   XP_077505338
VERSION     XP_077505338.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649212.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1117
                     /product="cohesin release factor wings apart-like"
                     /calculated_mol_wt=120160
     Region          562..929
                     /region_name="WAPL"
                     /note="Wings apart-like protein regulation of
                     heterochromatin; pfam07814"
                     /db_xref="CDD:462278"
     CDS             1..1117
                     /gene="wapl"
                     /coded_by="XM_077649212.1:148..3501"
                     /db_xref="GeneID:144115073"
ORIGIN      
        1 mskfgaktyg rkhncrlaps ldqiildkgd sktssakmsv aavhrwgmts ftslrkapqa
       61 ngqpdgandg paasdltavr ssfdddpfsf dsdrddtsrs tkgalshvhs tvqetaqtsk
      121 qplppkpkkf fksrndtcas slasqtsara veqfdklras tssnvrasks reknepaskv
      181 giswgpddnv dfrcvkleks sraaalsrdh shsvtastna alptagllts asltvapsas
      241 ipltavplag splsatplta vplataalaa asvtvaappa lqelpppsss afvieepakg
      301 sepcggndks sfrappairt ysrkrnqggs vetpgmvipv krtasavnvt damlsglvlm
      361 vpstnlgvts cvmgsgeaas ssevpfdnsa pvntgrsdkp laawqdadav tsnsslsssr
      421 dpepccvvsg etsrasatys qdspsgsqns apssqedvtq psepkasssr sifkskkifq
      481 spkkpkavyk mrhwqtpdvd eelvdteaae dqskhfgsfd dewfgdaalt kklcppsqpw
      541 kdtvtsvkcp rkarklytvv rnvkkayqch esgetqefnd didyllegvq psnpigarcl
      601 svvglaskcm vpafrmhlra hgimynffsa ltdapsdpsl slctatlffv lsqdrltmdl
      661 dastlslmlq lfesdpqqge ggleatqhps sdlterhrek vqqlceqmqq kghakhlnln
      721 rvntgtlale tllsltsrra gewfkeelrl qgglthivnt vsdcltyfgk gvtvlknptd
      781 fqleklkkid rccrvlenvt ymntdnqryl lefkdclvva syvrllllcq sslivhpvvn
      841 epgagssagg skssegqits vvlqnmqgpg avffscllsv vrvllnlthd salgsgivgg
      901 qpgllpaill cilqvpkavp leqrfdlqvl slgllinlle hsaenrkrla dtqtvgsfes
      961 vcdqmempaf nalmdlftdk ieaaqqseeq anellssqvq kvkasleprd gdsvpanqpp
     1021 vasqsddlee tlmkalqkag khmehsivca yialllgcav qnnkelterl rehtpdgkfl
     1081 plvealqkfh nfinltgvlg ntapkriqrv ievleds
//
LOCUS       XP_077505339            1117 aa            linear   INV 25-NOV-2025
DEFINITION  cohesin release factor wings apart-like [Amblyomma americanum].
ACCESSION   XP_077505339
VERSION     XP_077505339.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649213.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1117
                     /product="cohesin release factor wings apart-like"
                     /calculated_mol_wt=120160
     Region          562..929
                     /region_name="WAPL"
                     /note="Wings apart-like protein regulation of
                     heterochromatin; pfam07814"
                     /db_xref="CDD:462278"
     CDS             1..1117
                     /gene="wapl"
                     /coded_by="XM_077649213.1:197..3550"
                     /db_xref="GeneID:144115073"
ORIGIN      
        1 mskfgaktyg rkhncrlaps ldqiildkgd sktssakmsv aavhrwgmts ftslrkapqa
       61 ngqpdgandg paasdltavr ssfdddpfsf dsdrddtsrs tkgalshvhs tvqetaqtsk
      121 qplppkpkkf fksrndtcas slasqtsara veqfdklras tssnvrasks reknepaskv
      181 giswgpddnv dfrcvkleks sraaalsrdh shsvtastna alptagllts asltvapsas
      241 ipltavplag splsatplta vplataalaa asvtvaappa lqelpppsss afvieepakg
      301 sepcggndks sfrappairt ysrkrnqggs vetpgmvipv krtasavnvt damlsglvlm
      361 vpstnlgvts cvmgsgeaas ssevpfdnsa pvntgrsdkp laawqdadav tsnsslsssr
      421 dpepccvvsg etsrasatys qdspsgsqns apssqedvtq psepkasssr sifkskkifq
      481 spkkpkavyk mrhwqtpdvd eelvdteaae dqskhfgsfd dewfgdaalt kklcppsqpw
      541 kdtvtsvkcp rkarklytvv rnvkkayqch esgetqefnd didyllegvq psnpigarcl
      601 svvglaskcm vpafrmhlra hgimynffsa ltdapsdpsl slctatlffv lsqdrltmdl
      661 dastlslmlq lfesdpqqge ggleatqhps sdlterhrek vqqlceqmqq kghakhlnln
      721 rvntgtlale tllsltsrra gewfkeelrl qgglthivnt vsdcltyfgk gvtvlknptd
      781 fqleklkkid rccrvlenvt ymntdnqryl lefkdclvva syvrllllcq sslivhpvvn
      841 epgagssagg skssegqits vvlqnmqgpg avffscllsv vrvllnlthd salgsgivgg
      901 qpgllpaill cilqvpkavp leqrfdlqvl slgllinlle hsaenrkrla dtqtvgsfes
      961 vcdqmempaf nalmdlftdk ieaaqqseeq anellssqvq kvkasleprd gdsvpanqpp
     1021 vasqsddlee tlmkalqkag khmehsivca yialllgcav qnnkelterl rehtpdgkfl
     1081 plvealqkfh nfinltgvlg ntapkriqrv ievleds
//
LOCUS       XP_077505340             599 aa            linear   INV 25-NOV-2025
DEFINITION  protein NDNF-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505340
VERSION     XP_077505340.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649214.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..599
                     /product="protein NDNF-like isoform X1"
                     /calculated_mol_wt=67888
     Region          225..352
                     /region_name="NDNF"
                     /note="Neuron-derived neurotrophic factor, first Fn(III)
                     domain; pfam10179"
                     /db_xref="CDD:462981"
     Region          415..590
                     /region_name="NDNF_C"
                     /note="Neuron-derived Neurotrophic Factor C-terminal;
                     pfam19433"
                     /db_xref="CDD:466079"
     CDS             1..599
                     /gene="LOC144115076"
                     /coded_by="XM_077649214.1:191..1990"
                     /db_xref="GeneID:144115076"
ORIGIN      
        1 mikvivvgli sqlfnalrlq fgrtmpwlal llclllrgea avavdlrrps spsrpesqrp
       61 fhfakhrpel fydnnvvpen gevsvfvfkd qirrvfflve kernslllta spcsapiewh
      121 ifrkplapsd yseesnesrd psallyrddg astepvvggs fygrgrlsfs rrdaprgvym
      181 lefdsdsadt svrvygtsda dahypfpllp vkpkvdvlqv rngrvlftwk pspsegtthq
      241 dvqycvaanr kdnfhtkcav dsalrgdvps pphpdsgfgf wwekatrrkl alrsremqrg
      301 vrpqsdvvfe cvgrrtwhsf adlevdqryy idvfalnvkt nsstaypgts vvarsvgnqr
      361 iqdnqlvriv ldqrdnfaht akysvnanvp tvwmfvqscs gpgpiqlsvt lrkakvltae
      421 vmdtrtltln hpsngtyvik isstvpqlrr vhllvskkyh kfpfptlpdd ngvkvmgslt
      481 tcnsvtlawk aaldekvryc ifkqevrgsy sgvfaraqdf cdetklaeks gkvscrryhr
      541 fskhrfqnvi mqkirklqpd ttyvfevlvt kargrtlaye kvwatthrsc sssyrkgkr
//
LOCUS       XP_077505341             575 aa            linear   INV 25-NOV-2025
DEFINITION  protein NDNF-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505341
VERSION     XP_077505341.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649215.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..575
                     /product="protein NDNF-like isoform X2"
                     /calculated_mol_wt=65189
     Region          201..328
                     /region_name="NDNF"
                     /note="Neuron-derived neurotrophic factor, first Fn(III)
                     domain; pfam10179"
                     /db_xref="CDD:462981"
     Region          391..566
                     /region_name="NDNF_C"
                     /note="Neuron-derived Neurotrophic Factor C-terminal;
                     pfam19433"
                     /db_xref="CDD:466079"
     CDS             1..575
                     /gene="LOC144115076"
                     /coded_by="XM_077649215.1:372..2099"
                     /db_xref="GeneID:144115076"
ORIGIN      
        1 mpwlalllcl llrgeaavav dlrrpsspsr pesqrpfhfa khrpelfydn nvvpengevs
       61 vfvfkdqirr vfflvekern sllltaspcs apiewhifrk plapsdysee snesrdpsal
      121 lyrddgaste pvvggsfygr grlsfsrrda prgvymlefd sdsadtsvrv ygtsdadahy
      181 pfpllpvkpk vdvlqvrngr vlftwkpsps egtthqdvqy cvaanrkdnf htkcavdsal
      241 rgdvpspphp dsgfgfwwek atrrklalrs remqrgvrpq sdvvfecvgr rtwhsfadle
      301 vdqryyidvf alnvktnsst aypgtsvvar svgnqriqdn qlvrivldqr dnfahtakys
      361 vnanvptvwm fvqscsgpgp iqlsvtlrka kvltaevmdt rtltlnhpsn gtyvikisst
      421 vpqlrrvhll vskkyhkfpf ptlpddngvk vmgslttcns vtlawkaald ekvrycifkq
      481 evrgsysgvf araqdfcdet klaeksgkvs crryhrfskh rfqnvimqki rklqpdttyv
      541 fevlvtkarg rtlayekvwa tthrscsssy rkgkr
//
LOCUS       XP_077505342             575 aa            linear   INV 25-NOV-2025
DEFINITION  protein NDNF-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505342
VERSION     XP_077505342.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649216.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..575
                     /product="protein NDNF-like isoform X2"
                     /calculated_mol_wt=65189
     Region          201..328
                     /region_name="NDNF"
                     /note="Neuron-derived neurotrophic factor, first Fn(III)
                     domain; pfam10179"
                     /db_xref="CDD:462981"
     Region          391..566
                     /region_name="NDNF_C"
                     /note="Neuron-derived Neurotrophic Factor C-terminal;
                     pfam19433"
                     /db_xref="CDD:466079"
     CDS             1..575
                     /gene="LOC144115076"
                     /coded_by="XM_077649216.1:308..2035"
                     /db_xref="GeneID:144115076"
ORIGIN      
        1 mpwlalllcl llrgeaavav dlrrpsspsr pesqrpfhfa khrpelfydn nvvpengevs
       61 vfvfkdqirr vfflvekern sllltaspcs apiewhifrk plapsdysee snesrdpsal
      121 lyrddgaste pvvggsfygr grlsfsrrda prgvymlefd sdsadtsvrv ygtsdadahy
      181 pfpllpvkpk vdvlqvrngr vlftwkpsps egtthqdvqy cvaanrkdnf htkcavdsal
      241 rgdvpspphp dsgfgfwwek atrrklalrs remqrgvrpq sdvvfecvgr rtwhsfadle
      301 vdqryyidvf alnvktnsst aypgtsvvar svgnqriqdn qlvrivldqr dnfahtakys
      361 vnanvptvwm fvqscsgpgp iqlsvtlrka kvltaevmdt rtltlnhpsn gtyvikisst
      421 vpqlrrvhll vskkyhkfpf ptlpddngvk vmgslttcns vtlawkaald ekvrycifkq
      481 evrgsysgvf araqdfcdet klaeksgkvs crryhrfskh rfqnvimqki rklqpdttyv
      541 fevlvtkarg rtlayekvwa tthrscsssy rkgkr
//
LOCUS       XP_077505343             575 aa            linear   INV 25-NOV-2025
DEFINITION  protein NDNF-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505343
VERSION     XP_077505343.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649217.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..575
                     /product="protein NDNF-like isoform X2"
                     /calculated_mol_wt=65189
     Region          201..328
                     /region_name="NDNF"
                     /note="Neuron-derived neurotrophic factor, first Fn(III)
                     domain; pfam10179"
                     /db_xref="CDD:462981"
     Region          391..566
                     /region_name="NDNF_C"
                     /note="Neuron-derived Neurotrophic Factor C-terminal;
                     pfam19433"
                     /db_xref="CDD:466079"
     CDS             1..575
                     /gene="LOC144115076"
                     /coded_by="XM_077649217.1:253..1980"
                     /db_xref="GeneID:144115076"
ORIGIN      
        1 mpwlalllcl llrgeaavav dlrrpsspsr pesqrpfhfa khrpelfydn nvvpengevs
       61 vfvfkdqirr vfflvekern sllltaspcs apiewhifrk plapsdysee snesrdpsal
      121 lyrddgaste pvvggsfygr grlsfsrrda prgvymlefd sdsadtsvrv ygtsdadahy
      181 pfpllpvkpk vdvlqvrngr vlftwkpsps egtthqdvqy cvaanrkdnf htkcavdsal
      241 rgdvpspphp dsgfgfwwek atrrklalrs remqrgvrpq sdvvfecvgr rtwhsfadle
      301 vdqryyidvf alnvktnsst aypgtsvvar svgnqriqdn qlvrivldqr dnfahtakys
      361 vnanvptvwm fvqscsgpgp iqlsvtlrka kvltaevmdt rtltlnhpsn gtyvikisst
      421 vpqlrrvhll vskkyhkfpf ptlpddngvk vmgslttcns vtlawkaald ekvrycifkq
      481 evrgsysgvf araqdfcdet klaeksgkvs crryhrfskh rfqnvimqki rklqpdttyv
      541 fevlvtkarg rtlayekvwa tthrscsssy rkgkr
//
LOCUS       XP_077505344             205 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115077 [Amblyomma americanum].
ACCESSION   XP_077505344
VERSION     XP_077505344.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649218.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..205
                     /product="uncharacterized protein LOC144115077"
                     /calculated_mol_wt=21554
     Region          <8..>95
                     /region_name="DEAD-like_helicase_N"
                     /note="N-terminal helicase domain of the DEAD-box helicase
                     superfamily; cl28899"
                     /db_xref="CDD:475120"
     Site            12..18
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:350670"
     Region          <166..182
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     CDS             1..205
                     /gene="LOC144115077"
                     /coded_by="XM_077649218.1:915..1532"
                     /db_xref="GeneID:144115077"
ORIGIN      
        1 masilatara qtepdmtaav tpvsamalgd rsfqpeqmpv avlllptpkp tvqtakgtrk
       61 csdgsilktg hvcggttvkh hqalrfhgcy illattilak lmlpqelvds kvqsafvmge
      121 ageaalhpvl hsastgghas rqktsshgvq sgvahaliva kavpdrgldi kdvrhvinyd
      181 lpcslhgpha fswglrlghm ltqln
//
LOCUS       XP_077505345             403 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier organic anion transporter family member 4A1-like
            [Amblyomma americanum].
ACCESSION   XP_077505345
VERSION     XP_077505345.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649219.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..403
                     /product="solute carrier organic anion transporter family
                     member 4A1-like"
                     /calculated_mol_wt=43142
     Region          32..>346
                     /region_name="OATP"
                     /note="Organic Anion Transporter Polypeptide (OATP)
                     family; pfam03137"
                     /db_xref="CDD:460821"
     CDS             1..403
                     /gene="LOC144115078"
                     /coded_by="XM_077649219.1:351..1562"
                     /db_xref="GeneID:144115078"
ORIGIN      
        1 mytvharsrp fwrvlassrp vkgavvvdgv qviqsmfnva scimvtsgsy hdgvghrpvi
       61 pgigtlvtlv gamifaspdf iapqylalen rtanvcperg pgkcslfgra tnantfkyff
      121 mlshamhgvg sspfftvgvt yldqnvptgs asvymaaklk avevaqkqkq kqqtkqsrgt
      181 pdaqelkaae apeagddake qkdapspqqe apepkdvhsp qtnagsgqtp evaersfvnh
      241 vrrlvcnptf vcltlaaaae tmlasgltgf atkifiamfg isssqasgll gavpsacggt
      301 llggyvttkk lnvksptimc ycvilsfvpw ftlfvfmhsc dnnqsalanr tsppgaraci
      361 cssacpamhf lclawcdmlv mpvllvttwl vlqlllfrqr yti
//
LOCUS       XP_077505346             149 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115079 [Amblyomma americanum].
ACCESSION   XP_077505346
VERSION     XP_077505346.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649220.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..149
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..149
                     /product="uncharacterized protein LOC144115079"
                     /calculated_mol_wt=16638
     CDS             1..149
                     /gene="LOC144115079"
                     /coded_by="XM_077649220.1:74..523"
                     /db_xref="GeneID:144115079"
ORIGIN      
        1 mwlllmrsct sqddfrmkrg lvsscidlfv sfgkemasdd dlgdlpscpf icvkgwtflt
       61 ansyticmdt hpvlhaskpd eafkllffah fafniqyqke rslcleftqr aiaginparg
      121 tkvqknggkq hclsprvaal atalkdydf
//
LOCUS       XP_077505347             403 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier organic anion transporter family member 4A1-like
            [Amblyomma americanum].
ACCESSION   XP_077505347
VERSION     XP_077505347.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649221.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..403
                     /product="solute carrier organic anion transporter family
                     member 4A1-like"
                     /calculated_mol_wt=43133
     Region          32..>365
                     /region_name="OATP"
                     /note="Organic Anion Transporter Polypeptide (OATP)
                     family; pfam03137"
                     /db_xref="CDD:460821"
     CDS             1..403
                     /gene="LOC144115081"
                     /coded_by="XM_077649221.1:7..1218"
                     /db_xref="GeneID:144115081"
ORIGIN      
        1 mhtvharsrs fwrvlawsrp vkgavvvdgv qviqsmfnva scimvtsgsy hdgvghrpvi
       61 pgigtlvapv gamifaspdf iapqyrvlen gtanigperg pgkcslsgra tnantfkyff
      121 mlshamhgvg ssafftlgva yldqnvptgs asvymaaklk avevaqkqkq kqqwkqsrgt
      181 tdaqqlkaae apepgddake qedapspqqe apepkdvqsp qtnagsgqtp evaersfvnh
      241 vrrlvcnptf vcltlaaaae tmvasgltgf atkifiamfg isssqasgli gavpsacggt
      301 llggyvitrk lnaksptivr ycvilslvpw ftlfvfmhss dnnqstlanr tsppgaraci
      361 cssaclamhf lclawcdmlv mpvllvttwl vlqqllfrqr yti
//
LOCUS       XP_077505348             400 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier organic anion transporter family member 4A1-like
            isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505348
VERSION     XP_077505348.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649222.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..400
                     /product="solute carrier organic anion transporter family
                     member 4A1-like isoform X1"
                     /calculated_mol_wt=43071
     Region          32..>365
                     /region_name="OATP"
                     /note="Organic Anion Transporter Polypeptide (OATP)
                     family; pfam03137"
                     /db_xref="CDD:460821"
     CDS             1..400
                     /gene="LOC144115082"
                     /coded_by="XM_077649222.1:601..1803"
                     /db_xref="GeneID:144115082"
ORIGIN      
        1 mytvharsrp fwrvlassrp vkgavvvdgv qviqsmfnva scimvtsgsy hdgvghrpvi
       61 pgigtlvtlv gamifaspdf iapqylalen rtanvcperg pgkcslfgra tnantfkyff
      121 mlshamhgvg sspfftvgva yldqnvptgs asvymaaklk avevaqkqkq kqqwkqsrgt
      181 pdaqelkaae apeagddake qkdapspqqe apepkdvhsp qtnagsgqtp evaersfvnh
      241 vrrlvcnptf vcltlaaaae tmlasgltgf atkifiamfg isssqasgll gavpsacggt
      301 llggyvttkk lnvksptimc ycvilsfvpw ftlfvfmhsc dnnqstlanr tsppgarawi
      361 cssacpamhf lcwawcdmlv mpvntwlvlq lllfrqryti
//
LOCUS       XP_077505350             399 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier organic anion transporter family member 4A1-like
            isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505350
VERSION     XP_077505350.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649224.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..399
                     /product="solute carrier organic anion transporter family
                     member 4A1-like isoform X2"
                     /calculated_mol_wt=43000
     Region          32..>364
                     /region_name="OATP"
                     /note="Organic Anion Transporter Polypeptide (OATP)
                     family; pfam03137"
                     /db_xref="CDD:460821"
     CDS             1..399
                     /gene="LOC144115082"
                     /coded_by="XM_077649224.1:958..2157"
                     /db_xref="GeneID:144115082"
ORIGIN      
        1 mytvharsrp fwrvlassrp vkgavvvdgv qviqsmfnva scimvtsgsy hdgvghrpvi
       61 pgigtlvtlv gamifaspdf iapqylalen rtanvcperg pgkcslfgra tnantfkyff
      121 mlshamhgvg sspfftvgva yldqnvptgs asvymaklka vevaqkqkqk qqwkqsrgtp
      181 daqelkaaea peagddakeq kdapspqqea pepkdvhspq tnagsgqtpe vaersfvnhv
      241 rrlvcnptfv cltlaaaaet mlasgltgfa tkifiamfgi sssqasgllg avpsacggtl
      301 lggyvttkkl nvksptimcy cvilsfvpwf tlfvfmhscd nnqstlanrt sppgarawic
      361 ssacpamhfl cwawcdmlvm pvntwlvlql llfrqryti
//
LOCUS       XP_077505351             342 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115083 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505351
VERSION     XP_077505351.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649225.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..342
                     /product="uncharacterized protein LOC144115083 isoform X1"
                     /calculated_mol_wt=37276
     CDS             1..342
                     /gene="LOC144115083"
                     /coded_by="XM_077649225.1:233..1261"
                     /db_xref="GeneID:144115083"
ORIGIN      
        1 mparaaawpe pslqclvvfy rcavaderss lrdgvvatld salgsldgql arrkwdhghe
       61 hlcrparrvs cldwvmerhl hggqaqgnkg ehhailghag assclartvl avpggvlplc
      121 rrrraqqssg wsrghtyqrs rvsgwpagtw evgsrlrapv lactkselqs samteplrks
      181 asrksslsqg sshrlprqqr stslrdaatt rmllaasssn rrrlslqgvs gerddepekt
      241 gvgqtplprl ghgaafarrp gsrqqrrtpr dsrscrreql hgqnrpcsaw wcstvvpsqt
      301 saavfgmesw qhltalsalw maswhvgsgi tatstcaglh ee
//
LOCUS       XP_077505352             342 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115083 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505352
VERSION     XP_077505352.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649226.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..342
                     /product="uncharacterized protein LOC144115083 isoform X1"
                     /calculated_mol_wt=37276
     CDS             1..342
                     /gene="LOC144115083"
                     /coded_by="XM_077649226.1:321..1349"
                     /db_xref="GeneID:144115083"
ORIGIN      
        1 mparaaawpe pslqclvvfy rcavaderss lrdgvvatld salgsldgql arrkwdhghe
       61 hlcrparrvs cldwvmerhl hggqaqgnkg ehhailghag assclartvl avpggvlplc
      121 rrrraqqssg wsrghtyqrs rvsgwpagtw evgsrlrapv lactkselqs samteplrks
      181 asrksslsqg sshrlprqqr stslrdaatt rmllaasssn rrrlslqgvs gerddepekt
      241 gvgqtplprl ghgaafarrp gsrqqrrtpr dsrscrreql hgqnrpcsaw wcstvvpsqt
      301 saavfgmesw qhltalsalw maswhvgsgi tatstcaglh ee
//
LOCUS       XP_077505353             342 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115083 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505353
VERSION     XP_077505353.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649227.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..342
                     /product="uncharacterized protein LOC144115083 isoform X1"
                     /calculated_mol_wt=37276
     CDS             1..342
                     /gene="LOC144115083"
                     /coded_by="XM_077649227.1:362..1390"
                     /db_xref="GeneID:144115083"
ORIGIN      
        1 mparaaawpe pslqclvvfy rcavaderss lrdgvvatld salgsldgql arrkwdhghe
       61 hlcrparrvs cldwvmerhl hggqaqgnkg ehhailghag assclartvl avpggvlplc
      121 rrrraqqssg wsrghtyqrs rvsgwpagtw evgsrlrapv lactkselqs samteplrks
      181 asrksslsqg sshrlprqqr stslrdaatt rmllaasssn rrrlslqgvs gerddepekt
      241 gvgqtplprl ghgaafarrp gsrqqrrtpr dsrscrreql hgqnrpcsaw wcstvvpsqt
      301 saavfgmesw qhltalsalw maswhvgsgi tatstcaglh ee
//
LOCUS       XP_077505354             342 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115083 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505354
VERSION     XP_077505354.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649228.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..342
                     /product="uncharacterized protein LOC144115083 isoform X1"
                     /calculated_mol_wt=37276
     CDS             1..342
                     /gene="LOC144115083"
                     /coded_by="XM_077649228.1:219..1247"
                     /db_xref="GeneID:144115083"
ORIGIN      
        1 mparaaawpe pslqclvvfy rcavaderss lrdgvvatld salgsldgql arrkwdhghe
       61 hlcrparrvs cldwvmerhl hggqaqgnkg ehhailghag assclartvl avpggvlplc
      121 rrrraqqssg wsrghtyqrs rvsgwpagtw evgsrlrapv lactkselqs samteplrks
      181 asrksslsqg sshrlprqqr stslrdaatt rmllaasssn rrrlslqgvs gerddepekt
      241 gvgqtplprl ghgaafarrp gsrqqrrtpr dsrscrreql hgqnrpcsaw wcstvvpsqt
      301 saavfgmesw qhltalsalw maswhvgsgi tatstcaglh ee
//
LOCUS       XP_077505355             342 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115083 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505355
VERSION     XP_077505355.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649229.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..342
                     /product="uncharacterized protein LOC144115083 isoform X1"
                     /calculated_mol_wt=37276
     CDS             1..342
                     /gene="LOC144115083"
                     /coded_by="XM_077649229.1:393..1421"
                     /db_xref="GeneID:144115083"
ORIGIN      
        1 mparaaawpe pslqclvvfy rcavaderss lrdgvvatld salgsldgql arrkwdhghe
       61 hlcrparrvs cldwvmerhl hggqaqgnkg ehhailghag assclartvl avpggvlplc
      121 rrrraqqssg wsrghtyqrs rvsgwpagtw evgsrlrapv lactkselqs samteplrks
      181 asrksslsqg sshrlprqqr stslrdaatt rmllaasssn rrrlslqgvs gerddepekt
      241 gvgqtplprl ghgaafarrp gsrqqrrtpr dsrscrreql hgqnrpcsaw wcstvvpsqt
      301 saavfgmesw qhltalsalw maswhvgsgi tatstcaglh ee
//
LOCUS       XP_077505356             342 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115083 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505356
VERSION     XP_077505356.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649230.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..342
                     /product="uncharacterized protein LOC144115083 isoform X1"
                     /calculated_mol_wt=37276
     CDS             1..342
                     /gene="LOC144115083"
                     /coded_by="XM_077649230.1:837..1865"
                     /db_xref="GeneID:144115083"
ORIGIN      
        1 mparaaawpe pslqclvvfy rcavaderss lrdgvvatld salgsldgql arrkwdhghe
       61 hlcrparrvs cldwvmerhl hggqaqgnkg ehhailghag assclartvl avpggvlplc
      121 rrrraqqssg wsrghtyqrs rvsgwpagtw evgsrlrapv lactkselqs samteplrks
      181 asrksslsqg sshrlprqqr stslrdaatt rmllaasssn rrrlslqgvs gerddepekt
      241 gvgqtplprl ghgaafarrp gsrqqrrtpr dsrscrreql hgqnrpcsaw wcstvvpsqt
      301 saavfgmesw qhltalsalw maswhvgsgi tatstcaglh ee
//
LOCUS       XP_077505357             342 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115083 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505357
VERSION     XP_077505357.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649231.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..342
                     /product="uncharacterized protein LOC144115083 isoform X1"
                     /calculated_mol_wt=37276
     CDS             1..342
                     /gene="LOC144115083"
                     /coded_by="XM_077649231.1:233..1261"
                     /db_xref="GeneID:144115083"
ORIGIN      
        1 mparaaawpe pslqclvvfy rcavaderss lrdgvvatld salgsldgql arrkwdhghe
       61 hlcrparrvs cldwvmerhl hggqaqgnkg ehhailghag assclartvl avpggvlplc
      121 rrrraqqssg wsrghtyqrs rvsgwpagtw evgsrlrapv lactkselqs samteplrks
      181 asrksslsqg sshrlprqqr stslrdaatt rmllaasssn rrrlslqgvs gerddepekt
      241 gvgqtplprl ghgaafarrp gsrqqrrtpr dsrscrreql hgqnrpcsaw wcstvvpsqt
      301 saavfgmesw qhltalsalw maswhvgsgi tatstcaglh ee
//
LOCUS       XP_077505358             342 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115083 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505358
VERSION     XP_077505358.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649232.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..342
                     /product="uncharacterized protein LOC144115083 isoform X1"
                     /calculated_mol_wt=37276
     CDS             1..342
                     /gene="LOC144115083"
                     /coded_by="XM_077649232.1:233..1261"
                     /db_xref="GeneID:144115083"
ORIGIN      
        1 mparaaawpe pslqclvvfy rcavaderss lrdgvvatld salgsldgql arrkwdhghe
       61 hlcrparrvs cldwvmerhl hggqaqgnkg ehhailghag assclartvl avpggvlplc
      121 rrrraqqssg wsrghtyqrs rvsgwpagtw evgsrlrapv lactkselqs samteplrks
      181 asrksslsqg sshrlprqqr stslrdaatt rmllaasssn rrrlslqgvs gerddepekt
      241 gvgqtplprl ghgaafarrp gsrqqrrtpr dsrscrreql hgqnrpcsaw wcstvvpsqt
      301 saavfgmesw qhltalsalw maswhvgsgi tatstcaglh ee
//
LOCUS       XP_077505359             284 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115083 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505359
VERSION     XP_077505359.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649233.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..284
                     /product="uncharacterized protein LOC144115083 isoform X2"
                     /calculated_mol_wt=30805
     CDS             1..284
                     /gene="LOC144115083"
                     /coded_by="XM_077649233.1:213..1067"
                     /db_xref="GeneID:144115083"
ORIGIN      
        1 mssrrsdgtt tscldwvmer hlhggqaqgn kgehhailgh agassclart vlavpggvlp
       61 lcrrrraqqs sgwsrghtyq rsrvsgwpag twevgsrlra pvlactksel qssamteplr
      121 ksasrkssls qgsshrlprq qrstslrdaa ttrmllaass snrrrlslqg vsgerddepe
      181 ktgvgqtplp rlghgaafar rpgsrqqrrt prdsrscrre qlhgqnrpcs awwcstvvps
      241 qtsaavfgme swqhltalsa lwmaswhvgs gitatstcag lhee
//
LOCUS       XP_077505360             263 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115083 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505360
VERSION     XP_077505360.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649234.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..263
                     /product="uncharacterized protein LOC144115083 isoform X3"
                     /calculated_mol_wt=28713
     CDS             1..263
                     /gene="LOC144115083"
                     /coded_by="XM_077649234.1:233..1024"
                     /db_xref="GeneID:144115083"
ORIGIN      
        1 mparaaawpe pslqclvvfy rcavaderss lrdgvvatld salgsldgql arrkwdhghe
       61 hlcrparrvs cldwvmerhl hggqaqgnkg ehhailghag assclartvl avpggvlplc
      121 rrrraqqssg wsrghtyqrs rvsgwpagtw evgsrlrapv lactkselpr lghgaafarr
      181 pgsrqqrrtp rdsrscrreq lhgqnrpcsa wwcstvvpsq tsaavfgmes wqhltalsal
      241 wmaswhvgsg itatstcagl hee
//
LOCUS       XP_077505361             171 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115083 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505361
VERSION     XP_077505361.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649235.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..171
                     /product="uncharacterized protein LOC144115083 isoform X4"
                     /calculated_mol_wt=18510
     CDS             1..171
                     /gene="LOC144115083"
                     /coded_by="XM_077649235.1:233..748"
                     /db_xref="GeneID:144115083"
ORIGIN      
        1 mparaaawpe pslqclvvfy rcavaderss lrdgvvatld salgsldgql arrkwdhghe
       61 hlcrparrvs cldwvmerhl hggqaqgnkg ehhailghag assclartvl avpggvlplc
      121 rrrraqqssg wsrghtyqrs rvsgwpagtw evgsrlrapv lactksegcy a
//
LOCUS       XP_077505363             310 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115085 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505363
VERSION     XP_077505363.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649237.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..310
                     /product="uncharacterized protein LOC144115085 isoform X1"
                     /calculated_mol_wt=32987
     CDS             1..310
                     /gene="LOC144115085"
                     /coded_by="XM_077649237.1:8..940"
                     /db_xref="GeneID:144115085"
ORIGIN      
        1 mllniqpgac pgktclsiag relpwrrscr ylgllidrhl twrpalkafc rqatrvrgai
       61 qsllsggngi tpqmglrffq atagaqflya lplvqvtinq qlaiersqra avhlclgllr
      121 gsqipatlve agvcpmvlha qrpsgrhsam lsvstsprtd vpswsassai lihewgrlpr
      181 sssswwegsq saflplhdrt waicsgcsch hvepsrsasp qqeaaatrqe aggssdpggg
      241 lgllhasihg rvgphgclpv crsylhctfi grepagqapv rgqlehssag grppgcrhpg
      301 vpelqsaail
//
LOCUS       XP_077505364             135 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115085 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505364
VERSION     XP_077505364.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649238.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..135
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..135
                     /product="uncharacterized protein LOC144115085 isoform X2"
                     /calculated_mol_wt=14735
     CDS             1..135
                     /gene="LOC144115085"
                     /coded_by="XM_077649238.1:34..441"
                     /db_xref="GeneID:144115085"
ORIGIN      
        1 mglwhqarkq sqerentqkq rnitvseggv rasrrlsqnc grptrskmem sstcarllfp
       61 hgaqpcgghc ahaprshlgg sgtsdmfser dtvkrqcrig deatahlrrs ppcrptlsve
      121 igdhaispvv ehlat
//
LOCUS       XP_077505365             289 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115086 [Amblyomma americanum].
ACCESSION   XP_077505365
VERSION     XP_077505365.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649239.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..289
                     /product="uncharacterized protein LOC144115086"
                     /calculated_mol_wt=31166
     Region          12..>112
                     /region_name="DEAD-like_helicase_N"
                     /note="N-terminal helicase domain of the DEAD-box helicase
                     superfamily; cl28899"
                     /db_xref="CDD:475120"
     Site            38..44
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:350670"
     CDS             1..289
                     /gene="LOC144115086"
                     /coded_by="XM_077649239.1:903..1772"
                     /db_xref="GeneID:144115086"
ORIGIN      
        1 mclrfhplrs lryakpipvq ayavdtamvc refaraptgs gtnaaftpls dsssadlsyq
       61 reqrlvaais pplrklliqt aqyaskcahd silknafvfc dgaqqrsttr hcasaavsag
      121 fihysvmlsa vvlepphhkt gssrascfsk saalrasqaa rpgvrcqgsh gqqerhgscp
      181 gwvhhdrrid aaqrqrltrl flpagtgava viplptcqla iqtaecagkc ahdsipktaa
      241 llcdgttveh hqalperfwa sscshrydrq srgvlrccle pgrratavp
//
LOCUS       XP_077505366             285 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115087 [Amblyomma americanum].
ACCESSION   XP_077505366
VERSION     XP_077505366.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649240.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..285
                     /product="uncharacterized protein LOC144115087"
                     /calculated_mol_wt=31267
     Region          72..>180
                     /region_name="DEAD-like_helicase_N"
                     /note="N-terminal helicase domain of the DEAD-box helicase
                     superfamily; cl28899"
                     /db_xref="CDD:475120"
     Site            106..112
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:350670"
     CDS             1..285
                     /gene="LOC144115087"
                     /coded_by="XM_077649240.1:16..873"
                     /db_xref="GeneID:144115087"
ORIGIN      
        1 mlasivftlq cvscilctar ihvspqpaew rhhccqltwq fishtaiatf lerqrrktrr
       61 wfgarfrgqf vsiqdlclrf hllrslryak pipaqayavd tamvstgsgt naaftplsds
      121 ssadlsyqre qrlvaaispp lrklllqtaq yaskcahdsi lknafvfcdg aqqrsttrhc
      181 asaaasagfi hysvmlsavv lepphhktgs srascfsksa alhasqaarp gvrcqgshgq
      241 qerhgscpgw vhhdrridaa qrqrltrlfl pagtgavavt hdpvp
//
LOCUS       XP_077505367             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115089 [Amblyomma americanum].
ACCESSION   XP_077505367
VERSION     XP_077505367.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649241.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144115089"
                     /calculated_mol_wt=23237
     Region          12..>112
                     /region_name="DEAD-like_helicase_N"
                     /note="N-terminal helicase domain of the DEAD-box helicase
                     superfamily; cl28899"
                     /db_xref="CDD:475120"
     Site            38..44
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:350670"
     CDS             1..217
                     /gene="LOC144115089"
                     /coded_by="XM_077649241.1:2672..3325"
                     /db_xref="GeneID:144115089"
ORIGIN      
        1 mclrfhplrs lryakpipvq ayavdtamvc rdfaraptgs gtnaaftpls dsssadlsyq
       61 reqrlvaais pplrmlliqt aqyaskcahd silknafvfc dgaqqrsttr hcasaaasag
      121 fihysvmlsa vvlepphhkt gssrascfsk saalhasqga rpgvrcqgsh gqqerhgscp
      181 gwvhhdrhid aaqrqrltrl flpagtgava vthdpvp
//
LOCUS       XP_077505368             203 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115090 [Amblyomma americanum].
ACCESSION   XP_077505368
VERSION     XP_077505368.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649242.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..203
                     /product="uncharacterized protein LOC144115090"
                     /calculated_mol_wt=21475
     Region          1..>169
                     /region_name="OATP"
                     /note="Organic Anion Transporter Polypeptide (OATP)
                     family; pfam03137"
                     /db_xref="CDD:460821"
     CDS             1..203
                     /gene="LOC144115090"
                     /coded_by="XM_077649242.1:8..619"
                     /db_xref="GeneID:144115090"
ORIGIN      
        1 mhgvgsspff tvgvayldqn vpsgsasvym aaklkaveva qkqkqkqqsk qsrgtpdaqe
       61 lkaaeapeag dyakehkdap spqqeapepk dvqspqtnag sgqtpkvaer sfvnhvrrlv
      121 cnttfvcltl aaaaetmvas gltgfatkif iamfgisssq asgflgtfaa hcsetssdae
      181 rmliyknmrt pdfahftqsa ivl
//
LOCUS       XP_077505370             183 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115091 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505370
VERSION     XP_077505370.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649244.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="uncharacterized protein LOC144115091 isoform X1"
                     /calculated_mol_wt=19338
     CDS             1..183
                     /gene="LOC144115091"
                     /coded_by="XM_077649244.1:161..712"
                     /db_xref="GeneID:144115091"
ORIGIN      
        1 mvstgsgtna aftplsdsss adlsyqreqr lvaaispplr klllqtaqya skcahdsilk
       61 nafvfcdgaq qrsttrhcas aaasagfihy svmlsavvle pphhktgssr ascfsksaal
      121 hasqaarpgv rcqgshgqqe rhgscpgwvh hdrridaaqr qrltrlflpa gtgavavthd
      181 pvp
//
LOCUS       XP_077505371             111 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115091 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505371
VERSION     XP_077505371.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649245.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..111
                     /product="uncharacterized protein LOC144115091 isoform X2"
                     /calculated_mol_wt=12130
     Region          <2..66
                     /region_name="OATP"
                     /note="Organic Anion Transporter Polypeptide (OATP)
                     family; pfam03137"
                     /db_xref="CDD:460821"
     CDS             1..111
                     /gene="LOC144115091"
                     /coded_by="XM_077649245.1:216..551"
                     /db_xref="GeneID:144115091"
ORIGIN      
        1 makrdpcvsd cslvfvyaga ifialfftfl lrvatltall rstdesikst gfgvnyvair
       61 llgklprfll levcgapckp srpsrrtlsr lpwptgtpwl vprlgpsrps h
//
LOCUS       XP_077505372            3702 aa            linear   INV 25-NOV-2025
DEFINITION  sushi, von Willebrand factor type A, EGF and pentraxin
            domain-containing protein uif isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505372
VERSION     XP_077505372.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649246.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..3702
                     /product="sushi, von Willebrand factor type A, EGF and
                     pentraxin domain-containing protein uif isoform X1"
                     /calculated_mol_wt=404474
     Region          179..310
                     /region_name="CLECT"
                     /note="C-type lectin (CTL) or carbohydrate-recognition
                     domain (CRD); smart00034"
                     /db_xref="CDD:214480"
     Site            order(283,286,288,294,297..300,303..305)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     Region          314..348
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(319,327,338..339)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(331,334,338,344..345)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            341..345
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Site            order(359,362,364,391,396,434,458,460)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     Region          362..461
                     /region_name="CUB"
                     /note="Domain first found in C1r, C1s, uEGF, and bone
                     morphogenetic protein; smart00042"
                     /db_xref="CDD:214483"
     Region          468..579
                     /region_name="CUB"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; cd00041"
                     /db_xref="CDD:238001"
     Site            order(477,480,482,509,514,550,574,576,578..579)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     Region          581..693
                     /region_name="CUB"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; cd00041"
                     /db_xref="CDD:238001"
     Site            order(589,591,593,621,626,664,688,690,692..693)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     Region          714..933
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     Region          977..1124
                     /region_name="FA58C"
                     /note="Coagulation factor 5/8 C-terminal domain, discoidin
                     domain; Cell surface-attached carbohydrate-binding domain,
                     present in eukaryotes and assumed to have horizontally
                     transferred to eubacterial genomes; cd00057"
                     /db_xref="CDD:238014"
     Site            order(1014,1044,1051)
                     /site_type="other"
                     /note="sugar binding site [chemical binding]"
                     /db_xref="CDD:238014"
     Region          1110..>1260
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     Region          1199..1255
                     /region_name="CCP"
                     /note="Complement control protein (CCP) modules (aka short
                     consensus repeats SCRs or SUSHI repeats) have been
                     identified in several proteins of the complement system;
                     cd00033"
                     /db_xref="CDD:153056"
     Site            order(1209,1228)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     Region          1334..1393
                     /region_name="Sushi"
                     /note="Sushi repeat (SCR repeat); pfam00084"
                     /db_xref="CDD:459664"
     Region          1450..1590
                     /region_name="FA58C"
                     /note="Coagulation factor 5/8 C-terminal domain, discoidin
                     domain; Cell surface-attached carbohydrate-binding domain,
                     present in eukaryotes and assumed to have horizontally
                     transferred to eubacterial genomes; cl25480"
                     /db_xref="CDD:330301"
     Site            order(1486,1513,1517)
                     /site_type="other"
                     /note="sugar binding site [chemical binding]"
                     /db_xref="CDD:238014"
     Region          1610..1693
                     /region_name="HYR"
                     /note="HYR domain; pfam02494"
                     /db_xref="CDD:460572"
     Region          1694..1777
                     /region_name="HYR"
                     /note="HYR domain; pfam02494"
                     /db_xref="CDD:460572"
     Region          2013..2059
                     /region_name="Ephrin_rec_like"
                     /note="Tyrosine-protein kinase ephrin type A/B
                     receptor-like; pfam07699"
                     /db_xref="CDD:429604"
     Region          2066..2116
                     /region_name="Ephrin_rec_like"
                     /note="Tyrosine-protein kinase ephrin type A/B
                     receptor-like; pfam07699"
                     /db_xref="CDD:429604"
     Region          2123..2170
                     /region_name="Ephrin_rec_like"
                     /note="Tyrosine-protein kinase ephrin type A/B
                     receptor-like; pfam07699"
                     /db_xref="CDD:429604"
     Region          2209..2245
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(2209,2212,2226)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          2288..2326
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          2328..2363
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(2328,2331,2345)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          2403..2439
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(2403,2406,2420)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          2442..2477
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          2480..2517
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          2519..2555
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(2519,2522,2536)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          2561..2591
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     Region          2601..2631
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          <2639..2668
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          2723..2882
                     /region_name="Laminin_G_3"
                     /note="Concanavalin A-like lectin/glucanases superfamily;
                     pfam13385"
                     /db_xref="CDD:463865"
     Region          2946..3026
                     /region_name="HYR"
                     /note="HYR domain; pfam02494"
                     /db_xref="CDD:460572"
     Region          3258..3309
                     /region_name="Ephrin_rec_like"
                     /note="Tyrosine-protein kinase ephrin type A/B
                     receptor-like; pfam07699"
                     /db_xref="CDD:429604"
     Region          3316..3363
                     /region_name="Ephrin_rec_like"
                     /note="Tyrosine-protein kinase ephrin type A/B
                     receptor-like; pfam07699"
                     /db_xref="CDD:429604"
     Region          <3379..3415
                     /region_name="Ephrin_rec_like"
                     /note="Tyrosine-protein kinase ephrin type A/B
                     receptor-like; pfam07699"
                     /db_xref="CDD:429604"
     Region          3423..3471
                     /region_name="Ephrin_rec_like"
                     /note="Tyrosine-protein kinase ephrin type A/B
                     receptor-like; pfam07699"
                     /db_xref="CDD:429604"
     CDS             1..3702
                     /gene="uif"
                     /coded_by="XM_077649246.1:371..11479"
                     /db_xref="GeneID:144115092"
ORIGIN      
        1 masrspslev rrnlvpylaa kkspprrqsr rqqpppspar ppqagpasqs krvvprgvvv
       61 palccslady attplgpscp clrvaglrgg dsvwqradrh ehrvtgcccs pdlsflvars
      121 rapterssps rgrmaaavmc ggsravgqlr srtlglvlvv lalgraasan idkggelrcq
      181 dgwsqdgvyc ykffsnqhsw rraeevcrry gsqlalilny nqnnftrsla ttglkdvpqs
      241 aywiglksvd dlstntless sgyfipkyvg hwaldqprpk dgqcvqarlt ertqewhlap
      301 ceallpficq vpacpegsyl csngkclnnr wmcdghddcg desdemncpk schfymqssg
      361 dsiqspnypn kyepnldckw tlegplgtgi vlqfsefete anfdtvqvls ggrteessvs
      421 lvtlsgkqdl nsksfmtasn lmiikfrtda saekrgfras wktepvkcgg elmaqpsaqv
      481 lnspmypepy pggleclwvi tappskiisl eivdldlepd kdfilvrdgs lpsdpllktl
      541 tgpasenpqf lmstrdrlyl yfrssfgdsr rgfsirfrsg cevditstsg nvsspafgvd
      601 kyppnqvcay ritrpgggpv slkfekfava pddfvqiydg sdtrngirlh pgqgytgtnr
      661 ptltltassg qmfvlftsnp lntaagwqas fsadcplltv gegalassre ttfgakvtyv
      721 cpagqefsng mskivaecmp ggkwsigsip rcqerycgav pqidsgfava atnvtyrgqa
      781 tyqcyagfaf tggfptqtir cneegswvnl ptclasscpp lpevphtvht ilngggknyg
      841 tvvrfecepg yirtgypvvv cksdgqwsaa pptckraqcl dlpdikngfv ldsrrtyyfe
      901 deakiqchkg yrlegshvvt cgpnqtfnnl ptcrdineca sssacdsast ictntaggff
      961 ckckkgfepn ldcrpvgdlg lsngaipdts ikvsgteggy hknnvrldgp rgwcgniprs
     1021 genwvqvdls aptvvrgfrt qtvvrddgsq afplsvriqy tddltdifrd fadptgkale
     1081 frltpnggsg lavvnlplpf earyirvliv eyvgapcmrl elmgcsrqdc ldvnecvhkn
     1141 ggcdqrcins pggfnclcnv gydlytsngt ngfnilpset gtrdgdmyri nktcvpkmcs
     1201 plespengql lstksiyhfg dtvhfqcnfg yvmlgtttlt ctsggnwngt vptcqiatcp
     1261 vldddatqgl avrtnaasdh lvfqknvtvs cnevgrplre tatagfrqcv ynptdgkpdy
     1321 wlsgaspecr rtdcgpppdt igatygfhvd tkyrasfffg cedtftlvgk sklndnvirc
     1381 qedgtwdfgd lrcegpvced pgrppdgyqi atsfeqgsev vfacdrpgyv pyttdpitcv
     1441 knaeckvvra lgltsgaipd sainatshrs nyepknirln satgwcgqqe aftyvtvdmr
     1501 kiyhikallv kgvvtndvvg rptelrffyk trenenfvvy fpnfnltsrd sknygeltvi
     1561 plplsvrary vilgivsynk npclkfelmg cedteeevll gydngysfcv dreppafinc
     1621 pshplvlert psghvpvnfs vphavdnsgr iarfevrpvg fkppavvfed tvveytaydf
     1681 ngniavctin itvpdytpps ltcpqsyvve lterqesylv nfnetrrfin atdksgnvei
     1741 tltpesavip iggyrnvtvt gtdrfgnqaf cyfqvsvqat tcvawslapp angvvnclpn
     1801 eentgyrcla tcnsgfkftd geqaktyeca nkeqwkpssi ipdcvpedtn qaaydviasv
     1861 eyraggalsa vclnhyvsyv styysslnrl lsdrcsainv qmdisfhnts irtrgeneit
     1921 igytlrivpv vrqrllydlc gstlglifdl svpstsavie pilnisaqnv ggqcptiqai
     1981 rstvdrgfnc eagevlnqdv tgqvprclhc pagtfaskde rctfcsrgfy qdltrqssck
     2041 rcpvgthtrd egskslteci pvcgfgtysp tglvpclqcp sntfagtppi dgfkecqrcp
     2101 antftytpga dslksckprc ppgtysetgl epcspcpinf yqpqdgqtsc ikcapnqkta
     2161 rpgalspeac spvhcnpsic qngglcvaqn heavcycpag ftgqfcesdm necdstpcyn
     2221 ggtcvdqpqg ykckcppgyt glqcqeeqsd clnntcpdra mcqdlpgqgt vnclcrsgye
     2281 gptcnitvnp ctvgdspcan gglcvplqqg rykcqcplgw sgptcqinid dcaenpcllg
     2341 anctdlvndf vcdcpagftg krchqkvelc nvdpcvhgtc idrlfyseci chpgwegqtc
     2401 erniddcrsn pctnngvcid ginefrcqcd tgytgskcqh tiddcesspc qnggtcfdqi
     2461 egficqcrpg yvglqceaev decisspcyp tgtercvdsd naykcicnpg ytgelcevni
     2521 necesnpcln dgkctdaing fkcfcppgwt gerceidvgg casdpclnda rcidlfedyf
     2581 cvcpsgtdgk rcqtspqrci gnpcthggtc rdygsglnct cppgysgdgc qyehkacsdv
     2641 crngatcnev gnsvqcicap gftgphcesd vqdctpsscp psatcidlts gfyckcpfnl
     2701 tgedcrkpin idydlyinde srsssaalat sfelgskslt iamwiqyntp ssigtfftly
     2761 svespylpig krklvmaddt gvmvslfpdl tsdiyipyld nvpindgqwh hiviiwdgsh
     2821 gtltlitdta vagmvtgyih qehlpdfgwv slgaplneen katansgfhg risrvniwdr
     2881 pldfttevph qfrscknapv lfdglllrwt gydrvdgtve rqgpgkcgqr vcgvgftgde
     2941 crvrhqdktp pktvycppdm wvvtpntsvv lqweepqfsd dygslrvher ngvrtgqafm
     3001 kgtydlsyva ldeagnaaqc dfrihvlsef cplpapplgg qrhcsdwgpg srfkmctisc
     3061 ddgqefsqri pefyvcgaeg fwrptdspgd qlvfpacspk rpaqrifrla mnfpsavvcs
     3121 dsgkkilhsr ilesllqvnr nwricvdpsp gscngirvnv ncnkhsrlvr rqlddsdvys
     3181 vevsfpahnd pvvnvntqek stvkkivena vlqqsafdvr dtlpnvspdl tslsmvtdya
     3241 cpagevvvap fcvkcslgty ynnatqscrs cpvgfyqndv gqlackpcpv iagkqgvtsg
     3301 pgarsagqck ercstgkyfd eslglcrscg ygmyqprega fscmscgpgl ttrtneavsv
     3361 qecreecaag lqlsltgqcd pcpkgsyrtr gmaacnscpp gtttpnfgst sidqcslevc
     3421 rvghylnsst eecvpcpkgt yqdeeqrdle ckscpsdttt dkmgstsrth csdpcvvnge
     3481 rrncqehafc vfleerqdyd cqcmprfrli ndsgecvdvc edycenggrc eisqetsepr
     3541 cacqgnfygd rcekksefvy ivggiagavi fviliilliw micvrtswkn kpkklpqppp
     3601 mdisgsqsnf yygapapyae siapshhsty ahyydddddg wempnfynet ymkdglhngk
     3661 sgtlgrsnas iygtkedlyd rlrrhqyqgs kkdtsdsedq gq
//
LOCUS       XP_077505373            3371 aa            linear   INV 25-NOV-2025
DEFINITION  sushi, von Willebrand factor type A, EGF and pentraxin
            domain-containing protein uif isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505373
VERSION     XP_077505373.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649247.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..3371
                     /product="sushi, von Willebrand factor type A, EGF and
                     pentraxin domain-containing protein uif isoform X2"
                     /calculated_mol_wt=368134
     Site            order(28,31,33,60,65,103,127,129)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     Region          31..130
                     /region_name="CUB"
                     /note="Domain first found in C1r, C1s, uEGF, and bone
                     morphogenetic protein; smart00042"
                     /db_xref="CDD:214483"
     Region          137..248
                     /region_name="CUB"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; cd00041"
                     /db_xref="CDD:238001"
     Site            order(146,149,151,178,183,219,243,245,247..248)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     Region          250..362
                     /region_name="CUB"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; cd00041"
                     /db_xref="CDD:238001"
     Site            order(258,260,262,290,295,333,357,359,361..362)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     Region          383..602
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     Region          646..793
                     /region_name="FA58C"
                     /note="Coagulation factor 5/8 C-terminal domain, discoidin
                     domain; Cell surface-attached carbohydrate-binding domain,
                     present in eukaryotes and assumed to have horizontally
                     transferred to eubacterial genomes; cd00057"
                     /db_xref="CDD:238014"
     Site            order(683,713,720)
                     /site_type="other"
                     /note="sugar binding site [chemical binding]"
                     /db_xref="CDD:238014"
     Region          779..>929
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     Region          868..924
                     /region_name="CCP"
                     /note="Complement control protein (CCP) modules (aka short
                     consensus repeats SCRs or SUSHI repeats) have been
                     identified in several proteins of the complement system;
                     cd00033"
                     /db_xref="CDD:153056"
     Site            order(878,897)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     Region          1003..1062
                     /region_name="Sushi"
                     /note="Sushi repeat (SCR repeat); pfam00084"
                     /db_xref="CDD:459664"
     Region          1119..1259
                     /region_name="FA58C"
                     /note="Coagulation factor 5/8 C-terminal domain, discoidin
                     domain; Cell surface-attached carbohydrate-binding domain,
                     present in eukaryotes and assumed to have horizontally
                     transferred to eubacterial genomes; cl25480"
                     /db_xref="CDD:330301"
     Site            order(1155,1182,1186)
                     /site_type="other"
                     /note="sugar binding site [chemical binding]"
                     /db_xref="CDD:238014"
     Region          1279..1362
                     /region_name="HYR"
                     /note="HYR domain; pfam02494"
                     /db_xref="CDD:460572"
     Region          1363..1446
                     /region_name="HYR"
                     /note="HYR domain; pfam02494"
                     /db_xref="CDD:460572"
     Region          1682..1728
                     /region_name="Ephrin_rec_like"
                     /note="Tyrosine-protein kinase ephrin type A/B
                     receptor-like; pfam07699"
                     /db_xref="CDD:429604"
     Region          1735..1785
                     /region_name="Ephrin_rec_like"
                     /note="Tyrosine-protein kinase ephrin type A/B
                     receptor-like; pfam07699"
                     /db_xref="CDD:429604"
     Region          1792..1839
                     /region_name="Ephrin_rec_like"
                     /note="Tyrosine-protein kinase ephrin type A/B
                     receptor-like; pfam07699"
                     /db_xref="CDD:429604"
     Region          1878..1914
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(1878,1881,1895)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          1957..1995
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          1997..2032
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(1997,2000,2014)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          2072..2108
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(2072,2075,2089)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          2111..2146
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          2149..2186
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          2188..2224
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(2188,2191,2205)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          2230..2260
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     Region          2270..2300
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          <2308..2337
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          2392..2551
                     /region_name="Laminin_G_3"
                     /note="Concanavalin A-like lectin/glucanases superfamily;
                     pfam13385"
                     /db_xref="CDD:463865"
     Region          2615..2695
                     /region_name="HYR"
                     /note="HYR domain; pfam02494"
                     /db_xref="CDD:460572"
     Region          2927..2978
                     /region_name="Ephrin_rec_like"
                     /note="Tyrosine-protein kinase ephrin type A/B
                     receptor-like; pfam07699"
                     /db_xref="CDD:429604"
     Region          2985..3032
                     /region_name="Ephrin_rec_like"
                     /note="Tyrosine-protein kinase ephrin type A/B
                     receptor-like; pfam07699"
                     /db_xref="CDD:429604"
     Region          <3048..3084
                     /region_name="Ephrin_rec_like"
                     /note="Tyrosine-protein kinase ephrin type A/B
                     receptor-like; pfam07699"
                     /db_xref="CDD:429604"
     Region          3092..3140
                     /region_name="Ephrin_rec_like"
                     /note="Tyrosine-protein kinase ephrin type A/B
                     receptor-like; pfam07699"
                     /db_xref="CDD:429604"
     CDS             1..3371
                     /gene="uif"
                     /coded_by="XM_077649247.1:3171..13286"
                     /db_xref="GeneID:144115092"
ORIGIN      
        1 mcdghddcgd esdemncpks chfymqssgd siqspnypnk yepnldckwt legplgtgiv
       61 lqfsefetea nfdtvqvlsg grteessvsl vtlsgkqdln sksfmtasnl miikfrtdas
      121 aekrgfrasw ktepvkcgge lmaqpsaqvl nspmypepyp ggleclwvit appskiisle
      181 ivdldlepdk dfilvrdgsl psdpllktlt gpasenpqfl mstrdrlyly frssfgdsrr
      241 gfsirfrsgc evditstsgn vsspafgvdk yppnqvcayr itrpgggpvs lkfekfavap
      301 ddfvqiydgs dtrngirlhp gqgytgtnrp tltltassgq mfvlftsnpl ntaagwqasf
      361 sadcplltvg egalassret tfgakvtyvc pagqefsngm skivaecmpg gkwsigsipr
      421 cqerycgavp qidsgfavaa tnvtyrgqat yqcyagfaft ggfptqtirc neegswvnlp
      481 tclasscppl pevphtvhti lngggknygt vvrfecepgy irtgypvvvc ksdgqwsaap
      541 ptckraqcld lpdikngfvl dsrrtyyfed eakiqchkgy rlegshvvtc gpnqtfnnlp
      601 tcrdinecas ssacdsasti ctntaggffc kckkgfepnl dcrpvgdlgl sngaipdtsi
      661 kvsgteggyh knnvrldgpr gwcgniprsg enwvqvdlsa ptvvrgfrtq tvvrddgsqa
      721 fplsvriqyt ddltdifrdf adptgkalef rltpnggsgl avvnlplpfe aryirvlive
      781 yvgapcmrle lmgcsrqdcl dvnecvhkng gcdqrcinsp ggfnclcnvg ydlytsngtn
      841 gfnilpsetg trdgdmyrin ktcvpkmcsp lespengqll stksiyhfgd tvhfqcnfgy
      901 vmlgtttltc tsggnwngtv ptcqiatcpv ldddatqgla vrtnaasdhl vfqknvtvsc
      961 nevgrplret atagfrqcvy nptdgkpdyw lsgaspecrr tdcgpppdti gatygfhvdt
     1021 kyrasfffgc edtftlvgks klndnvircq edgtwdfgdl rcegpvcedp grppdgyqia
     1081 tsfeqgsevv facdrpgyvp yttdpitcvk naeckvvral gltsgaipds ainatshrsn
     1141 yepknirlns atgwcgqqea ftyvtvdmrk iyhikallvk gvvtndvvgr ptelrffykt
     1201 renenfvvyf pnfnltsrds knygeltvip lplsvraryv ilgivsynkn pclkfelmgc
     1261 edteeevllg ydngysfcvd reppafincp shplvlertp sghvpvnfsv phavdnsgri
     1321 arfevrpvgf kppavvfedt vveytaydfn gniavctini tvpdytppsl tcpqsyvvel
     1381 terqesylvn fnetrrfina tdksgnveit ltpesavipi ggyrnvtvtg tdrfgnqafc
     1441 yfqvsvqatt cvawslappa ngvvnclpne entgyrclat cnsgfkftdg eqaktyecan
     1501 keqwkpssii pdcvpedtnq aaydviasve yraggalsav clnhyvsyvs tyysslnrll
     1561 sdrcsainvq mdisfhntsi rtrgeneiti gytlrivpvv rqrllydlcg stlglifdls
     1621 vpstsaviep ilnisaqnvg gqcptiqair stvdrgfnce agevlnqdvt gqvprclhcp
     1681 agtfaskder ctfcsrgfyq dltrqssckr cpvgthtrde gsksltecip vcgfgtyspt
     1741 glvpclqcps ntfagtppid gfkecqrcpa ntftytpgad slksckprcp pgtysetgle
     1801 pcspcpinfy qpqdgqtsci kcapnqktar pgalspeacs pvhcnpsicq ngglcvaqnh
     1861 eavcycpagf tgqfcesdmn ecdstpcyng gtcvdqpqgy kckcppgytg lqcqeeqsdc
     1921 lnntcpdram cqdlpgqgtv nclcrsgyeg ptcnitvnpc tvgdspcang glcvplqqgr
     1981 ykcqcplgws gptcqinidd caenpcllga nctdlvndfv cdcpagftgk rchqkvelcn
     2041 vdpcvhgtci drlfysecic hpgwegqtce rniddcrsnp ctnngvcidg inefrcqcdt
     2101 gytgskcqht iddcesspcq nggtcfdqie gficqcrpgy vglqceaevd ecisspcypt
     2161 gtercvdsdn aykcicnpgy tgelcevnin ecesnpclnd gkctdaingf kcfcppgwtg
     2221 erceidvggc asdpclndar cidlfedyfc vcpsgtdgkr cqtspqrcig npcthggtcr
     2281 dygsglnctc ppgysgdgcq yehkacsdvc rngatcnevg nsvqcicapg ftgphcesdv
     2341 qdctpsscpp satcidltsg fyckcpfnlt gedcrkpini dydlyindes rsssaalats
     2401 felgskslti amwiqyntps sigtfftlys vespylpigk rklvmaddtg vmvslfpdlt
     2461 sdiyipyldn vpindgqwhh iviiwdgshg tltlitdtav agmvtgyihq ehlpdfgwvs
     2521 lgaplneenk atansgfhgr isrvniwdrp ldfttevphq frscknapvl fdglllrwtg
     2581 ydrvdgtver qgpgkcgqrv cgvgftgdec rvrhqdktpp ktvycppdmw vvtpntsvvl
     2641 qweepqfsdd ygslrvhern gvrtgqafmk gtydlsyval deagnaaqcd frihvlsefc
     2701 plpapplggq rhcsdwgpgs rfkmctiscd dgqefsqrip efyvcgaegf wrptdspgdq
     2761 lvfpacspkr paqrifrlam nfpsavvcsd sgkkilhsri lesllqvnrn wricvdpspg
     2821 scngirvnvn cnkhsrlvrr qlddsdvysv evsfpahndp vvnvntqeks tvkkivenav
     2881 lqqsafdvrd tlpnvspdlt slsmvtdyac pagevvvapf cvkcslgtyy nnatqscrsc
     2941 pvgfyqndvg qlackpcpvi agkqgvtsgp garsagqcke rcstgkyfde slglcrscgy
     3001 gmyqpregaf scmscgpglt trtneavsvq ecreecaagl qlsltgqcdp cpkgsyrtrg
     3061 maacnscppg tttpnfgsts idqcslevcr vghylnsste ecvpcpkgty qdeeqrdlec
     3121 kscpsdtttd kmgstsrthc sdpcvvnger rncqehafcv fleerqdydc qcmprfrlin
     3181 dsgecvdvce dycenggrce isqetseprc acqgnfygdr cekksefvyi vggiagavif
     3241 viliilliwm icvrtswknk pkklpqpppm disgsqsnfy ygapapyaes iapshhstya
     3301 hyydddddgw empnfynety mkdglhngks gtlgrsnasi ygtkedlydr lrrhqyqgsk
     3361 kdtsdsedqg q
//
LOCUS       XP_077505374             911 aa            linear   INV 25-NOV-2025
DEFINITION  protein bicaudal C isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505374
VERSION     XP_077505374.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649248.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..911
                     /product="protein bicaudal C isoform X1"
                     /calculated_mol_wt=99761
     Region          46..123
                     /region_name="KH-I"
                     /note="K homology (KH) RNA-binding domain, type I;
                     cl00098"
                     /db_xref="CDD:469614"
     Region          128..197
                     /region_name="KH-I_BICC1_rpt2"
                     /note="second type I K homology (KH) RNA-binding domain
                     found in protein bicaudal C homolog 1 (BICC1) and similar
                     proteins; cd22421"
                     /db_xref="CDD:411849"
     Site            order(139,141..143,145..149,152..153,156..157,162..165)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411849"
     Region          283..375
                     /region_name="KH-I"
                     /note="K homology (KH) RNA-binding domain, type I;
                     cl00098"
                     /db_xref="CDD:469614"
     Region          827..884
                     /region_name="SAM_superfamily"
                     /note="SAM (Sterile alpha motif); cl15755"
                     /db_xref="CDD:472832"
     CDS             1..911
                     /gene="BicC"
                     /coded_by="XM_077649248.1:10..2745"
                     /db_xref="GeneID:144115093"
ORIGIN      
        1 mrrvacpcst crgtmeslev tsthssefdg ssivgdesdp anayverirv drkkleemlq
       61 etdakastsa dgffkkvmet tdtiiswptk lkanvkskkd phvkvcgssq avgkaresil
      121 evldtkrnrv tmkmdvsftd hsyiigkggh niqqvmdntg chihfpdsnr nshmdksnqv
      181 siagtpngve sarnqirell pmvvsfelpl nlalptaldp tsmalqgilk shgitvtfrp
      241 qqnsltlvvv vkgsrryvdr lrqgllvlve yltggtrtqv glvtlsmeia phhhgfvmgh
      301 gncnvqqiar qtgcvitfpd pvavtsqppp ilpafmalgq qtcpqgrppv gipskstvqi
      361 tgsfdavhna wlelmgrlpl vlmfdlkenq avdmlaiykl mdhmnvhisi kpkpkrnsrs
      421 avvrcpegds rllfevrrii lsldtdelgs pspmdkipys takpshhgcm mpepmwweiq
      481 aasqhhaaaa avaarqqqqq qqqqqqqlfl ngllagspfi ghpqlmtgsl qqqqqaallp
      541 lllgqlfqgp rpalfepsmg ysltgrpptc iapserlpsp khssscghss sahscpgsqa
      601 spmdsgygfs lhdeghlsts deatschtwe dsvndmegke swkkshehgs sssgiqgspe
      661 eswidypmfe yqrgmgpvgc erkvtfgnpl phleyerrkl latramqehv gctkrvpssi
      721 wsgfgfsksm pesmvkgqfq qcsasttsgr qpkleetkvc ssidqatpsq vwdddgdacl
      781 gatgvamvnd sdngqhsarn ldaafsassf fdyvgarcqp qdnscmdiph lfsrlglgky
      841 vdifqqneid lptflcmsds dlqqlsipya arkkmllais dlnspglsaf srnippgpge
      901 meflsdrfcr l
//
LOCUS       XP_077505375             910 aa            linear   INV 25-NOV-2025
DEFINITION  protein bicaudal C isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505375
VERSION     XP_077505375.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649249.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..910
                     /product="protein bicaudal C isoform X2"
                     /calculated_mol_wt=99690
     Region          46..123
                     /region_name="KH-I"
                     /note="K homology (KH) RNA-binding domain, type I;
                     cl00098"
                     /db_xref="CDD:469614"
     Region          128..197
                     /region_name="KH-I_BICC1_rpt2"
                     /note="second type I K homology (KH) RNA-binding domain
                     found in protein bicaudal C homolog 1 (BICC1) and similar
                     proteins; cd22421"
                     /db_xref="CDD:411849"
     Site            order(139,141..143,145..149,152..153,156..157,162..165)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411849"
     Region          283..375
                     /region_name="KH-I"
                     /note="K homology (KH) RNA-binding domain, type I;
                     cl00098"
                     /db_xref="CDD:469614"
     Region          826..883
                     /region_name="SAM_superfamily"
                     /note="SAM (Sterile alpha motif); cl15755"
                     /db_xref="CDD:472832"
     CDS             1..910
                     /gene="BicC"
                     /coded_by="XM_077649249.1:10..2742"
                     /db_xref="GeneID:144115093"
ORIGIN      
        1 mrrvacpcst crgtmeslev tsthssefdg ssivgdesdp anayverirv drkkleemlq
       61 etdakastsa dgffkkvmet tdtiiswptk lkanvkskkd phvkvcgssq avgkaresil
      121 evldtkrnrv tmkmdvsftd hsyiigkggh niqqvmdntg chihfpdsnr nshmdksnqv
      181 siagtpngve sarnqirell pmvvsfelpl nlalptaldp tsmalqgilk shgitvtfrp
      241 qqnsltlvvv vkgsrryvdr lrqgllvlve yltggtrtqv glvtlsmeia phhhgfvmgh
      301 gncnvqqiar qtgcvitfpd pvavtsqppp ilpafmalgq qtcpqgrppv gipskstvqi
      361 tgsfdavhna wlelmgrlpl vlmfdlkenq avdmlaiykl mdhmnvhisi kpkpkrnsrs
      421 avvrcpegds rllfevrrii lsldtdelgs pspmdkipys takpshhgcm mpepmwweiq
      481 asqhhaaaaa vaarqqqqqq qqqqqqlfln gllagspfig hpqlmtgslq qqqqaallpl
      541 llgqlfqgpr palfepsmgy sltgrpptci apserlpspk hssscghsss ahscpgsqas
      601 pmdsgygfsl hdeghlstsd eatschtwed svndmegkes wkkshehgss ssgiqgspee
      661 swidypmfey qrgmgpvgce rkvtfgnplp hleyerrkll atramqehvg ctkrvpssiw
      721 sgfgfsksmp esmvkgqfqq csasttsgrq pkleetkvcs sidqatpsqv wdddgdaclg
      781 atgvamvnds dngqhsarnl daafsassff dyvgarcqpq dnscmdiphl fsrlglgkyv
      841 difqqneidl ptflcmsdsd lqqlsipyaa rkkmllaisd lnspglsafs rnippgpgem
      901 eflsdrfcrl
//
LOCUS       XP_077505376             831 aa            linear   INV 25-NOV-2025
DEFINITION  protein bicaudal C isoform X3 [Amblyomma americanum].
ACCESSION   XP_077505376
VERSION     XP_077505376.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649250.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..831
                     /product="protein bicaudal C isoform X3"
                     /calculated_mol_wt=90976
     Region          46..123
                     /region_name="KH-I"
                     /note="K homology (KH) RNA-binding domain, type I;
                     cl00098"
                     /db_xref="CDD:469614"
     Region          128..197
                     /region_name="KH-I_BICC1_rpt2"
                     /note="second type I K homology (KH) RNA-binding domain
                     found in protein bicaudal C homolog 1 (BICC1) and similar
                     proteins; cd22421"
                     /db_xref="CDD:411849"
     Site            order(139,141..143,145..149,152..153,156..157,162..165)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411849"
     Region          203..295
                     /region_name="KH-I"
                     /note="K homology (KH) RNA-binding domain, type I;
                     cl00098"
                     /db_xref="CDD:469614"
     Region          747..804
                     /region_name="SAM_superfamily"
                     /note="SAM (Sterile alpha motif); cl15755"
                     /db_xref="CDD:472832"
     CDS             1..831
                     /gene="BicC"
                     /coded_by="XM_077649250.1:12..2507"
                     /db_xref="GeneID:144115093"
ORIGIN      
        1 mrrvacpcst crgtmeslev tsthssefdg ssivgdesdp anayverirv drkkleemlq
       61 etdakastsa dgffkkvmet tdtiiswptk lkanvkskkd phvkvcgssq avgkaresil
      121 evldtkrnrv tmkmdvsftd hsyiigkggh niqqvmdntg chihfpdsnr nshmdksnqv
      181 siagtpngve sarnqirtqv glvtlsmeia phhhgfvmgh gncnvqqiar qtgcvitfpd
      241 pvavtsqppp ilpafmalgq qtcpqgrppv gipskstvqi tgsfdavhna wlelmgrlpl
      301 vlmfdlkenq avdmlaiykl mdhmnvhisi kpkpkrnsrs avvrcpegds rllfevrrii
      361 lsldtdelgs pspmdkipys takpshhgcm mpepmwweiq aasqhhaaaa avaarqqqqq
      421 qqqqqqqlfl ngllagspfi ghpqlmtgsl qqqqqaallp lllgqlfqgp rpalfepsmg
      481 ysltgrpptc iapserlpsp khssscghss sahscpgsqa spmdsgygfs lhdeghlsts
      541 deatschtwe dsvndmegke swkkshehgs sssgiqgspe eswidypmfe yqrgmgpvgc
      601 erkvtfgnpl phleyerrkl latramqehv gctkrvpssi wsgfgfsksm pesmvkgqfq
      661 qcsasttsgr qpkleetkvc ssidqatpsq vwdddgdacl gatgvamvnd sdngqhsarn
      721 ldaafsassf fdyvgarcqp qdnscmdiph lfsrlglgky vdifqqneid lptflcmsds
      781 dlqqlsipya arkkmllais dlnspglsaf srnippgpge meflsdrfcr l
//
LOCUS       XP_077505377             784 aa            linear   INV 25-NOV-2025
DEFINITION  protein bicaudal C isoform X4 [Amblyomma americanum].
ACCESSION   XP_077505377
VERSION     XP_077505377.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649251.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..784
                     /product="protein bicaudal C isoform X4"
                     /calculated_mol_wt=86427
     Region          46..123
                     /region_name="KH-I"
                     /note="K homology (KH) RNA-binding domain, type I;
                     cl00098"
                     /db_xref="CDD:469614"
     Region          128..197
                     /region_name="KH-I_BICC1_rpt2"
                     /note="second type I K homology (KH) RNA-binding domain
                     found in protein bicaudal C homolog 1 (BICC1) and similar
                     proteins; cd22421"
                     /db_xref="CDD:411849"
     Site            order(139,141..143,145..149,152..153,156..157,162..165)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411849"
     Region          283..375
                     /region_name="KH-I"
                     /note="K homology (KH) RNA-binding domain, type I;
                     cl00098"
                     /db_xref="CDD:469614"
     Region          700..757
                     /region_name="SAM_superfamily"
                     /note="SAM (Sterile alpha motif); cl15755"
                     /db_xref="CDD:472832"
     CDS             1..784
                     /gene="BicC"
                     /coded_by="XM_077649251.1:10..2364"
                     /db_xref="GeneID:144115093"
ORIGIN      
        1 mrrvacpcst crgtmeslev tsthssefdg ssivgdesdp anayverirv drkkleemlq
       61 etdakastsa dgffkkvmet tdtiiswptk lkanvkskkd phvkvcgssq avgkaresil
      121 evldtkrnrv tmkmdvsftd hsyiigkggh niqqvmdntg chihfpdsnr nshmdksnqv
      181 siagtpngve sarnqirell pmvvsfelpl nlalptaldp tsmalqgilk shgitvtfrp
      241 qqnsltlvvv vkgsrryvdr lrqgllvlve yltggtrtqv glvtlsmeia phhhgfvmgh
      301 gncnvqqiar qtgcvitfpd pvavtsqppp ilpafmalgq qtcpqgrppv gipskstvqi
      361 tgsfdavhna wlelmgrlpl vlmfdlkenq avdmlaiykl mdhmnvhisi kpkpkrnsrs
      421 avvrcpegds rllfevrrii lsldtdelgs pspmdkipys takpshhgcm mpepmwweiq
      481 gfslhdeghl stsdeatsch twedsvndme gkeswkkshe hgssssgiqg speeswidyp
      541 mfeyqrgmgp vgcerkvtfg nplphleyer rkllatramq ehvgctkrvp ssiwsgfgfs
      601 ksmpesmvkg qfqqcsastt sgrqpkleet kvcssidqat psqvwdddgd aclgatgvam
      661 vndsdngqhs arnldaafsa ssffdyvgar cqpqdnscmd iphlfsrlgl gkyvdifqqn
      721 eidlptflcm sdsdlqqlsi pyaarkkmll aisdlnspgl safsrnippg pgemeflsdr
      781 fcrl
//
LOCUS       XP_077505378             522 aa            linear   INV 25-NOV-2025
DEFINITION  putative ATP-dependent RNA helicase DDX5 [Amblyomma americanum].
ACCESSION   XP_077505378
VERSION     XP_077505378.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649252.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..522
                     /product="putative ATP-dependent RNA helicase DDX5"
                     /calculated_mol_wt=58751
     Region          36..493
                     /region_name="DEAD-like_helicase_N"
                     /note="N-terminal helicase domain of the DEAD-box helicase
                     superfamily; cl28899"
                     /db_xref="CDD:475120"
     Site            order(143..149,253)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:350807"
     CDS             1..522
                     /gene="LOC144115094"
                     /coded_by="XM_077649252.1:137..1705"
                     /db_xref="GeneID:144115094"
ORIGIN      
        1 mfskivrqci pvasrgknty nswprdrdal wragntlgqt lvaprfdkvk lvpfeknfyv
       61 eseitaarse edvnafraeh eitvqghgrg gvpkpvltld ecnfpvecra lferknitqp
      121 spiqaqawpi vmsgrdlvgi aqtgsgktla yvlpsaihms rqqrpsglre gpisvvlapt
      181 relvqqisqv ayewcegafg ltgtpvyggv skspqierlr rgvhmcvatp grlldiletg
      241 avnllrctfl vldeadrmld mgfepqirki ieqirpdrqt vmwsatwpse vrslaqeflv
      301 pdhmqvtvgs adlcanhnik qvihvcdefe kerklldvle dimaegeqrt lvfvarksrv
      361 vhllqtlqkk gfravathgd lsqskrdial drfrsgatsi mvatdvaarg ldvtdikyvv
      421 nydypdtsen yvhrigrtgr rdqegtaitf ftpdnaaqak qliavlqead qevpqelwql
      481 vnlhvskqda qnrakkaqrq qswkrprryh hdeelaetrg wg
//
LOCUS       XP_077505379             477 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115095 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505379
VERSION     XP_077505379.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649253.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..477
                     /product="uncharacterized protein LOC144115095 isoform X1"
                     /calculated_mol_wt=54000
     CDS             1..477
                     /gene="LOC144115095"
                     /coded_by="XM_077649253.1:1946..3379"
                     /db_xref="GeneID:144115095"
ORIGIN      
        1 msgghypvqq qsgwnrirrr prpyerptpr yfsldwpetd tskkaednvd fhcdnlaaep
       61 rasgsavtgn hmstpthpkt qapvkpsgey rsrrmslavs spvidflaaa vptsenanev
      121 kiekqsprkk sdgkhseihh ehkpsdvlpv sylssafemt ipeeyklkpr dhsthvltag
      181 cdvntsvtnr sitavrppts fpadicrpte daqemnnist malpekrksk raysfplkgl
      241 elcaetqkha lsttmeddpr rgidiplvkh ekgqilaedi lahapspkrs hkssehqter
      301 vepiaifens rktsgeqpls wpeipvgvee tqgiekrhng etsktkgkkr kktsvpvqly
      361 dssdgqrgkd dvtlydvyls latemiedqr kqctyqegke vlkmlyeevv rnaarainav
      421 qrrhadklhk lneerdkira qrkefyrlgg mlkeyieekn ipgvhpyefc fteddci
//
LOCUS       XP_077505380             438 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115095 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505380
VERSION     XP_077505380.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649254.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..438
                     /product="uncharacterized protein LOC144115095 isoform X2"
                     /calculated_mol_wt=49217
     CDS             1..438
                     /gene="LOC144115095"
                     /coded_by="XM_077649254.1:204..1520"
                     /db_xref="GeneID:144115095"
ORIGIN      
        1 msskkaednv dfhcdnlaae prasgsavtg nhmstpthpk tqapvkpsge yrsrrmslav
       61 sspvidflaa avptsenane vkiekqsprk ksdgkhseih hehkpsdvlp vsylssafem
      121 tipeeyklkp rdhsthvlta gcdvntsvtn rsitavrppt sfpadicrpt edaqemnnis
      181 tmalpekrks kraysfplkg lelcaetqkh alsttmeddp rrgidiplvk hekgqilaed
      241 ilahapspkr shkssehqte rvepiaifen srktsgeqpl swpeipvgve etqgiekrhn
      301 getsktkgkk rkktsvpvql ydssdgqrgk ddvtlydvyl slatemiedq rkqctyqegk
      361 evlkmlyeev vrnaaraina vqrrhadklh klneerdkir aqrkefyrlg gmlkeyieek
      421 nipgvhpyef cfteddci
//
LOCUS       XP_077505381             403 aa            linear   INV 25-NOV-2025
DEFINITION  outer mitochondrial transmembrane helix translocase-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077505381
VERSION     XP_077505381.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649255.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..403
                     /product="outer mitochondrial transmembrane helix
                     translocase-like isoform X1"
                     /calculated_mol_wt=45088
     Region          105..270
                     /region_name="RecA-like_ATAD1"
                     /note="ATPase domain of ATPase family AAA
                     domain-containing protein 1 and similar ATPase domains;
                     cd19520"
                     /db_xref="CDD:410928"
     Site            order(106..109,148..154)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:410928"
     Region          294..329
                     /region_name="AAA_lid_3"
                     /note="AAA+ lid domain; pfam17862"
                     /db_xref="CDD:465537"
     CDS             1..403
                     /gene="LOC144115096"
                     /coded_by="XM_077649255.1:101..1312"
                     /db_xref="GeneID:144115096"
ORIGIN      
        1 masegvrmsl trgeitglvf rlslfgalty fgikwmvnai dptrkqkies kkradrilkk
       61 igiqnvnhat pvweparisy qlteyelsia aqlidprdin iswdsiagle eitqelretv
      121 ilpiqkrhlf tgsqliqppk gvllhgppgc gktmiakata reagarfinl evaaltdkwy
      181 gesqklasav ftlavkiqpc iifideidsf lrsrdsqdhe atammkaqfm clwdglitdp
      241 dcqvvvmgat nrphdvdkai lrrmpamfhv glpnqqqrag iiklvleteg vnkdvnivki
      301 arltegfsgs dlrelcrnaa lyrvrdllri ekhqggshke vsddeetfhd arqsgvregs
      361 dddetfhdal rpicmedftn alakmknskv lahqrhsapl eld
//
LOCUS       XP_077505382             389 aa            linear   INV 25-NOV-2025
DEFINITION  outer mitochondrial transmembrane helix translocase-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077505382
VERSION     XP_077505382.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649256.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..389
                     /product="outer mitochondrial transmembrane helix
                     translocase-like isoform X2"
                     /calculated_mol_wt=43451
     Region          91..256
                     /region_name="RecA-like_ATAD1"
                     /note="ATPase domain of ATPase family AAA
                     domain-containing protein 1 and similar ATPase domains;
                     cd19520"
                     /db_xref="CDD:410928"
     Site            order(92..95,134..140)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:410928"
     Region          280..315
                     /region_name="AAA_lid_3"
                     /note="AAA+ lid domain; pfam17862"
                     /db_xref="CDD:465537"
     CDS             1..389
                     /gene="LOC144115096"
                     /coded_by="XM_077649256.1:96..1265"
                     /db_xref="GeneID:144115096"
ORIGIN      
        1 masegvrmsl trgeitglvf rlslfgalty fgikwmvnai dptrkqkies kkradrilkk
       61 igiqnvnlte yelsiaaqli dprdiniswd siagleeitq elretvilpi qkrhlftgsq
      121 liqppkgvll hgppgcgktm iakatareag arfinlevaa ltdkwygesq klasavftla
      181 vkiqpciifi deidsflrsr dsqdheatam mkaqfmclwd glitdpdcqv vvmgatnrph
      241 dvdkailrrm pamfhvglpn qqqragiikl vletegvnkd vnivkiarlt egfsgsdlre
      301 lcrnaalyrv rdllriekhq ggshkevsdd eetfhdarqs gvregsddde tfhdalrpic
      361 medftnalak mknskvlahq rhsapleld
//
LOCUS       XP_077505383             261 aa            linear   INV 25-NOV-2025
DEFINITION  proliferating cell nuclear antigen [Amblyomma americanum].
ACCESSION   XP_077505383
VERSION     XP_077505383.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649257.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..261
                     /product="proliferating cell nuclear antigen"
                     /calculated_mol_wt=28627
     Region          1..259
                     /region_name="pcna"
                     /note="proliferating cell nuclear antigen (pcna);
                     TIGR00590"
                     /db_xref="CDD:273158"
     Site            order(13..14,20,77,80,146,149,210,217)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238322"
     Site            order(27,29,40,67,69,119..122,252..255)
                     /site_type="other"
                     /note="PCNA/WAF1-CIP1 protein binding site [polypeptide
                     binding]"
                     /db_xref="CDD:238322"
     Site            order(40,47,250)
                     /site_type="other"
                     /note="PCNA/FEN-1 protein interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:238322"
     Site            order(77,80..82,111..117,143,146..147,149..150,173,
                     176..177,179..183)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:238322"
     Site            order(208,252..254)
                     /site_type="other"
                     /note="PCNA/RFCL protein interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:238322"
     CDS             1..261
                     /gene="PCNA"
                     /coded_by="XM_077649257.1:138..923"
                     /db_xref="GeneID:144115097"
ORIGIN      
        1 mfearlvhga llkkvldavk dlinegtwdc satgislqam dvshvslvsl nlrsdgfdkf
       61 rcdrnlsmgm nltsvskilk caanndiiti kaqddadtvn fvfeapnqek vsdyemklmn
      121 idtehlgipe tdysvvikmp sgefqricrd lsqigdsvqi tctkdgvrfs vsgdlgtgnv
      181 klaqtanvdk edeaviietq eavsltfals ylnsftkatp lsaqvqlsms advplvveyk
      241 vedmgyvryy lapkiedsed n
//
LOCUS       XP_077505384             441 aa            linear   INV 25-NOV-2025
DEFINITION  CDP-diacylglycerol synthase [Amblyomma americanum].
ACCESSION   XP_077505384
VERSION     XP_077505384.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649258.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..441
                     /product="CDP-diacylglycerol synthase"
                     /calculated_mol_wt=51130
     Region          103..441
                     /region_name="CTP_transf_1"
                     /note="Cytidylyltransferase family; cl21502"
                     /db_xref="CDD:451277"
     CDS             1..441
                     /gene="Cds"
                     /coded_by="XM_077649258.1:237..1562"
                     /db_xref="GeneID:144115098"
ORIGIN      
        1 mselrkrttd peqkeskeha vdsddeklpe esqvdsltkd lpqatdetis ffdnclsglp
       61 krwknwvvrg iftlvmmfff afviwmgpla lmmltiliqv kcfqeiiaig yavyrvhglp
      121 wfrslswyfl lttnyflyge slvdyfgvli srtdslkplv ryhrfisfcm yllgfiwfvl
      181 slvkkyymrq fslfawthvt lviivtqsyf liqnmfegli wfiapvsmiv cndvmaymfg
      241 fffgrtplik lspkktwegf igggiatvvf gammsqylcq fkyfvcpiey seelermtmd
      301 cepsplfqpt dyelpgwiaa fchllhrktt itlypfvlhs lsfslfssii gpfggffasg
      361 fkrafkikdf gdiipghggi mdrfdcqilm asfvnvyyhs firapnqqki lqqiylmkpe
      421 aqlqifsslr dtllargllp s
//
LOCUS       XP_077505385             827 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115099 [Amblyomma americanum].
ACCESSION   XP_077505385
VERSION     XP_077505385.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649259.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..827
                     /product="uncharacterized protein LOC144115099"
                     /calculated_mol_wt=88681
     Region          717..809
                     /region_name="Spaetzle"
                     /note="pfam16077"
                     /db_xref="CDD:465007"
     CDS             1..827
                     /gene="LOC144115099"
                     /coded_by="XM_077649259.1:133..2616"
                     /db_xref="GeneID:144115099"
ORIGIN      
        1 mpprtklhwf llillatfei qceaeetttr ptssfklhrp lgfnrngtrt fnhtivtvrr
       61 kplfgsrrlk akqengaats teslqnvvpd ykksplegkd dapptttifp pppllrpspv
      121 esaanapqav ptepsfnpdt fftnrkknpv vvvrrfpkgs qgpnerddat sqgggggpps
      181 eagggngqpg llpianfprp lprpgygyqp aqpvfggsip ldvlgaggip rfftqrdidf
      241 vaqafrnaag nesipalnip tfnvpssnqt qqaeqtqggi vgaenqkqeq prlrvpenvl
      301 rqgyadglng ygnqprppld gsqpvvplna qqrpplgvdg rplvfqvpng ayrvayqpqa
      361 gpypnnvhpq lysgvvpnsg yppgqgddqa fnldprllrp nypivqgglq vpqrkqppfn
      421 palqqgppsr pgqqslagqi phgranpgll yqnylpvqnl asvsgftpsg aanlqagglq
      481 agglqaggya psgavprgfr fipipsndrv vaipdevfan sftpnqlvei rdgraipvqp
      541 vyavypgrnm mgyppsapkg gaerptpggv vrrplyqsey aggldippqa pqkrtgyiag
      601 repeaayspa ahdvmytyrp dvlpgcangs keavyclqdd eypraalhls vdleqntmhr
      661 llpetperdg naaaalfveg agpakdstaa asservsgsg ngtalppptl tddgslfaca
      721 srvtharplr artvdgfwkv vvnmhsphgr gafqqmvrte ecsepggscs nlvkssrcvq
      781 kynlhrlvvw trpegihmdt frlpvacscy lgkadkkatq rrgpgrt
//
LOCUS       XP_077505386             176 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115101 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505386
VERSION     XP_077505386.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649260.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..176
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..176
                     /product="uncharacterized protein LOC144115101 isoform X1"
                     /calculated_mol_wt=19741
     Region          125..145
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(125,128,141,145)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          153..169
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..176
                     /gene="LOC144115101"
                     /coded_by="XM_077649260.1:855..1385"
                     /db_xref="GeneID:144115101"
ORIGIN      
        1 mensgvipsv sakqmsppgs yeataasgng anigadhled agsyerliqe ivpdlkeqsv
       61 keealpdssk kqgaenragg eneqeeeree lpqrrrrrrr aifkrlgsvg rppyrakvke
      121 ptffchrcpa sfifkreley hivehtgrpl lpcefcprqf ptgrmlsshc arkhgr
//
LOCUS       XP_077505387             162 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115101 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505387
VERSION     XP_077505387.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649261.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..162
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..162
                     /product="uncharacterized protein LOC144115101 isoform X2"
                     /calculated_mol_wt=18313
     Region          111..131
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(111,114,127,131)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          139..155
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..162
                     /gene="LOC144115101"
                     /coded_by="XM_077649261.1:181..669"
                     /db_xref="GeneID:144115101"
ORIGIN      
        1 msppgsyeat aasgnganig adhledagsy erliqeivpd lkeqsvkeea lpdsskkqga
       61 enraggeneq eeereelpqr rrrrrraifk rlgsvgrppy rakvkeptff chrcpasfif
      121 kreleyhive htgrpllpce fcprqfptgr mlsshcarkh gr
//
LOCUS       XP_077505388             162 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115101 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505388
VERSION     XP_077505388.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649262.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..162
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..162
                     /product="uncharacterized protein LOC144115101 isoform X2"
                     /calculated_mol_wt=18313
     Region          111..131
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(111,114,127,131)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          139..155
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..162
                     /gene="LOC144115101"
                     /coded_by="XM_077649262.1:173..661"
                     /db_xref="GeneID:144115101"
ORIGIN      
        1 msppgsyeat aasgnganig adhledagsy erliqeivpd lkeqsvkeea lpdsskkqga
       61 enraggeneq eeereelpqr rrrrrraifk rlgsvgrppy rakvkeptff chrcpasfif
      121 kreleyhive htgrpllpce fcprqfptgr mlsshcarkh gr
//
LOCUS       XP_077505389             176 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115103 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505389
VERSION     XP_077505389.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649263.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..176
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..176
                     /product="uncharacterized protein LOC144115103 isoform X1"
                     /calculated_mol_wt=19138
     CDS             1..176
                     /gene="LOC144115103"
                     /coded_by="XM_077649263.1:808..1338"
                     /db_xref="GeneID:144115103"
ORIGIN      
        1 mrpifiigrr dgqprlgkgs lphggkgtat gapprrlcgi lwdrtassgs gqrrerllph
       61 aliifrdrrt gircalplyf rfqaakraqq tcalvdfpsk csgfgfaprm wharenpaai
      121 vgackcsppi lpkscpcpsr vsspcwfwsf gvlhlpgssa vctwltlpgf aparrt
//
LOCUS       XP_077505390             154 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115103 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505390
VERSION     XP_077505390.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649264.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..154
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..154
                     /product="uncharacterized protein LOC144115103 isoform X2"
                     /calculated_mol_wt=16646
     CDS             1..154
                     /gene="LOC144115103"
                     /coded_by="XM_077649264.1:4703..5167"
                     /db_xref="GeneID:144115103"
ORIGIN      
        1 mrpifiigrr dgqprlgkgs lphggkgtat gapprrlcgi lwdrtassgs gqrrerllph
       61 aliifrdrrt gircalplyf rfqaakraqq tcalvdfpsk csgfgfaprm wharenpaai
      121 vgackcsppi lpkscpcpsr getaaadlaw rgil
//
LOCUS       XP_077505391             111 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115104 [Amblyomma americanum].
ACCESSION   XP_077505391
VERSION     XP_077505391.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649265.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..111
                     /product="uncharacterized protein LOC144115104"
                     /calculated_mol_wt=12556
     Region          <5..105
                     /region_name="P2RX7_C"
                     /note="P2X purinoreceptor 7 intracellular domain;
                     pfam20478"
                     /db_xref="CDD:466627"
     CDS             1..111
                     /gene="LOC144115104"
                     /coded_by="XM_077649265.1:301..636"
                     /db_xref="GeneID:144115104"
ORIGIN      
        1 mptalestcc reipaatvkq psgcitehph fytlcldevv ltvalqmlld hglrvehtgr
       61 yryisysqla hwlwgrlgrh hrkvlpacav cqirerfpse gytgfqyaqg l
//
LOCUS       XP_077505393             117 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115105 [Amblyomma americanum].
ACCESSION   XP_077505393
VERSION     XP_077505393.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649267.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..117
                     /product="uncharacterized protein LOC144115105"
                     /calculated_mol_wt=12727
     CDS             1..117
                     /gene="LOC144115105"
                     /coded_by="XM_077649267.1:122..475"
                     /db_xref="GeneID:144115105"
ORIGIN      
        1 mrartqlail hfnenaskgq attaeglsrw kikhpkarkg tavacpvkae ptydyvglll
       61 ketvhcceqw psfkaafaen lstapppmsh sfprpsknel vaarrsrfas smgsttv
//
LOCUS       XP_077505394             176 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115108 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505394
VERSION     XP_077505394.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649268.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..176
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..176
                     /product="uncharacterized protein LOC144115108 isoform X1"
                     /calculated_mol_wt=19739
     Region          125..145
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(125,128,141,145)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          153..169
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..176
                     /gene="LOC144115108"
                     /coded_by="XM_077649268.1:855..1385"
                     /db_xref="GeneID:144115108"
ORIGIN      
        1 mensgvipsv sakqmsppgs yeataesgng anigadhled agscerliqe ivpdlkeqsv
       61 keealpdssk kqgaenragg eneqeeeree lpqrrrrrrr aifkrlgsvg rppyrakvke
      121 ptffchrcpa sfifkreley hivehtgrpl lpcefcprqf ptgrmlsshc arkhgr
//
LOCUS       XP_077505395             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115108 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505395
VERSION     XP_077505395.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649269.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144115108 isoform X2"
                     /calculated_mol_wt=13931
     CDS             1..127
                     /gene="LOC144115108"
                     /coded_by="XM_077649269.1:855..1238"
                     /db_xref="GeneID:144115108"
ORIGIN      
        1 mensgvipsv sakqmsppgs yeataesgng anigadhled agscerliqe ivpdlkeqsv
       61 keealpdssk kqgaenragg eneqeeeree lpqrrrrrrr aifkrlgsvg rppyrakakr
      121 ppqvllr
//
LOCUS       XP_077505396             117 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115111 [Amblyomma americanum].
ACCESSION   XP_077505396
VERSION     XP_077505396.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649270.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..117
                     /product="uncharacterized protein LOC144115111"
                     /calculated_mol_wt=12727
     CDS             1..117
                     /gene="LOC144115111"
                     /coded_by="XM_077649270.1:122..475"
                     /db_xref="GeneID:144115111"
ORIGIN      
        1 mrartqlail hfnenaskgq attaeglsrw kikhpkarkg tavacpvkae ptydyvglll
       61 ketvhcceqw psfkaafaen lstapppmsh sfprpsknel vaarrsrfas smgsttv
//
LOCUS       XP_077505397             202 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115113 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505397
VERSION     XP_077505397.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649271.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..202
                     /product="uncharacterized protein LOC144115113 isoform X1"
                     /calculated_mol_wt=21919
     CDS             1..202
                     /gene="LOC144115113"
                     /coded_by="XM_077649271.1:388..996"
                     /db_xref="GeneID:144115113"
ORIGIN      
        1 mhgpsrfllr vrrslagaea eqmgrsntea akalrchkpr lgkegslphg gkgtatgaap
       61 rrlcgilwdr tassgsgqrr erllphalii frdrrtgirc alplyfrfqa akraqqtcal
      121 vdfpskcsgf gfaprmwhar enpaaivgac kcsppilpks cpcpsrvssp cwfwsfgvlh
      181 lpgssavctw ltlpgfapar rt
//
LOCUS       XP_077505398             202 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115113 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505398
VERSION     XP_077505398.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649272.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..202
                     /product="uncharacterized protein LOC144115113 isoform X1"
                     /calculated_mol_wt=21919
     CDS             1..202
                     /gene="LOC144115113"
                     /coded_by="XM_077649272.1:391..999"
                     /db_xref="GeneID:144115113"
ORIGIN      
        1 mhgpsrfllr vrrslagaea eqmgrsntea akalrchkpr lgkegslphg gkgtatgaap
       61 rrlcgilwdr tassgsgqrr erllphalii frdrrtgirc alplyfrfqa akraqqtcal
      121 vdfpskcsgf gfaprmwhar enpaaivgac kcsppilpks cpcpsrvssp cwfwsfgvlh
      181 lpgssavctw ltlpgfapar rt
//
LOCUS       XP_077505400             202 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115113 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505400
VERSION     XP_077505400.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649274.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..202
                     /product="uncharacterized protein LOC144115113 isoform X1"
                     /calculated_mol_wt=21919
     CDS             1..202
                     /gene="LOC144115113"
                     /coded_by="XM_077649274.1:384..992"
                     /db_xref="GeneID:144115113"
ORIGIN      
        1 mhgpsrfllr vrrslagaea eqmgrsntea akalrchkpr lgkegslphg gkgtatgaap
       61 rrlcgilwdr tassgsgqrr erllphalii frdrrtgirc alplyfrfqa akraqqtcal
      121 vdfpskcsgf gfaprmwhar enpaaivgac kcsppilpks cpcpsrvssp cwfwsfgvlh
      181 lpgssavctw ltlpgfapar rt
//
LOCUS       XP_077505401             202 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115113 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505401
VERSION     XP_077505401.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649275.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..202
                     /product="uncharacterized protein LOC144115113 isoform X1"
                     /calculated_mol_wt=21919
     CDS             1..202
                     /gene="LOC144115113"
                     /coded_by="XM_077649275.1:309..917"
                     /db_xref="GeneID:144115113"
ORIGIN      
        1 mhgpsrfllr vrrslagaea eqmgrsntea akalrchkpr lgkegslphg gkgtatgaap
       61 rrlcgilwdr tassgsgqrr erllphalii frdrrtgirc alplyfrfqa akraqqtcal
      121 vdfpskcsgf gfaprmwhar enpaaivgac kcsppilpks cpcpsrvssp cwfwsfgvlh
      181 lpgssavctw ltlpgfapar rt
//
LOCUS       XP_077505402             202 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115113 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505402
VERSION     XP_077505402.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649276.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..202
                     /product="uncharacterized protein LOC144115113 isoform X1"
                     /calculated_mol_wt=21919
     CDS             1..202
                     /gene="LOC144115113"
                     /coded_by="XM_077649276.1:305..913"
                     /db_xref="GeneID:144115113"
ORIGIN      
        1 mhgpsrfllr vrrslagaea eqmgrsntea akalrchkpr lgkegslphg gkgtatgaap
       61 rrlcgilwdr tassgsgqrr erllphalii frdrrtgirc alplyfrfqa akraqqtcal
      121 vdfpskcsgf gfaprmwhar enpaaivgac kcsppilpks cpcpsrvssp cwfwsfgvlh
      181 lpgssavctw ltlpgfapar rt
//
LOCUS       XP_077505403             177 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115113 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505403
VERSION     XP_077505403.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649277.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..177
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..177
                     /product="uncharacterized protein LOC144115113 isoform X2"
                     /calculated_mol_wt=19241
     CDS             1..177
                     /gene="LOC144115113"
                     /coded_by="XM_077649277.1:4816..5349"
                     /db_xref="GeneID:144115113"
ORIGIN      
        1 mrpifiigrr dgqprlgkeg slphggkgta tgaaprrlcg ilwdrtassg sgqrrerllp
       61 haliifrdrr tgircalply frfqaakraq qtcalvdfps kcsgfgfapr mwharenpaa
      121 ivgackcspp ilpkscpcps rvsspcwfws fgvlhlpgss avctwltlpg faparrt
//
LOCUS       XP_077505404             136 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115114 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505404
VERSION     XP_077505404.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649278.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..136
                     /product="uncharacterized protein LOC144115114 isoform X1"
                     /calculated_mol_wt=14240
     CDS             1..136
                     /gene="LOC144115114"
                     /coded_by="XM_077649278.1:1013..1423"
                     /db_xref="GeneID:144115114"
ORIGIN      
        1 mdpsrrrgpy kkylwnagql eagghrrhst casildvran slpitsgllq leeklpretc
       61 rrwqasfaan sseasgfset pgssetpshc vsgtlspstl ddgqgstssa ascsdseedd
      121 pmdsstseid sagsgk
//
LOCUS       XP_077505405              55 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115114 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505405
VERSION     XP_077505405.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649279.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..55
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..55
                     /product="uncharacterized protein LOC144115114 isoform X2"
                     /calculated_mol_wt=5733
     CDS             1..55
                     /gene="LOC144115114"
                     /coded_by="XM_077649279.1:1059..1226"
                     /db_xref="GeneID:144115114"
ORIGIN      
        1 mqdnwklggi gdtqpvrasw mcaptacpsp qastgnlsal asiicsklqr gfrlq
//
LOCUS       XP_077505406             338 aa            linear   INV 25-NOV-2025
DEFINITION  NAD-dependent methylenetetrahydrofolate dehydrogenase [Amblyomma
            americanum].
ACCESSION   XP_077505406
VERSION     XP_077505406.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649280.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..338
                     /product="NAD-dependent methylenetetrahydrofolate
                     dehydrogenase"
                     /calculated_mol_wt=36272
     Region          45..337
                     /region_name="FolD"
                     /note="5,10-methylene-tetrahydrofolate
                     dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase
                     [Coenzyme transport and metabolism]; COG0190"
                     /db_xref="CDD:439960"
     CDS             1..338
                     /gene="Nmdmc"
                     /coded_by="XM_077649280.1:88..1104"
                     /db_xref="GeneID:144115116"
ORIGIN      
        1 mrrlklnlap nmhsfkalrf vrfvrncsgs ahqatspefq tarvadvidg rriareiese
       61 iraaidgils dgrrrprlta vlvgddegss vyvknkikaa ertgietdsl hlpstttqaa
      121 lldivrrlnd senvdgilvq lplpsqiees vvcnavlpsk dvdgfhalqm grlclnmssl
      181 apctplaive ilkrigvsty gkhvlvcgrs tivglpisim lgstrdygna tvsschsrth
      241 psdlkalakm adivitatgv pglitadmvk dgacvidvgi trvrdklsgk trlvgdvdfe
      301 gvsrkasfit pvpggvgpvt vamlmrntfl aysrainy
//
LOCUS       XP_077505407             968 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115117 [Amblyomma americanum].
ACCESSION   XP_077505407
VERSION     XP_077505407.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649281.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (GKSP01007081.1) annotated
            using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 67 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..968
                     /product="uncharacterized protein LOC144115117"
                     /calculated_mol_wt=105815
     Region          9..88
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cd00136"
                     /db_xref="CDD:467153"
     Site            order(18..24,36,39,69,73,76..77)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467153"
     Region          429..606
                     /region_name="Sec7"
                     /note="Sec7 domain; Domain named after the S. cerevisiae
                     SEC7 gene product. The Sec7 domain is the central domain
                     of the guanine-nucleotide-exchange factors (GEFs) of the
                     ADP-ribosylation factor family of small GTPases (ARFs) .
                     It carries the exchange factor...; cd00171"
                     /db_xref="CDD:238100"
     Site            order(506..513,542..555)
                     /site_type="active"
                     /note="active site/putative ARF binding site [active]"
                     /db_xref="CDD:238100"
     Region          646..771
                     /region_name="PH_EFA6"
                     /note="Exchange Factor for ARF6 Pleckstrin homology (PH)
                     domain; cd13295"
                     /db_xref="CDD:270107"
     CDS             1..968
                     /gene="LOC144115117"
                     /coded_by="XM_077649281.1:51..2957"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 67 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144115117"
ORIGIN      
        1 mddgelrsvq lkrsesfgfg isvfggsglg lppviydvad nspaafsgql eagdvivevn
       61 dtpvvsfttk evlkclrlan dvvtlkikkd paiqervrrq mmaaasserl kspaagepaa
      121 prqngtpggg gqqqqhvtaa aeeaggdeat sspstssssa avarkcelft mtgdliitka
      181 sstkaprtqs tpaspgggdp qrgtkreass lqspvaekkg sfgvrtsrsd dhlgkargtv
      241 aavsididdd emasslntll dtkddprvlw tynagssedn tattgsasss sgrrednlse
      301 lecctsemas afgseeadrv haseldlssp sqlspenedg fsreedwvae pdlsesqfrh
      361 palldsslra aargarrmqh srsngstasr tdasetleld sppsahsggt leeelaaqqd
      421 daatdeesda eslrsfhysp kavdmpsasr lakrlynleg fkksdvsrhl sknnefarvv
      481 aeeylrffef sgrtldtalr mflhrfclig etqerervlv hfskryldcn pgafksqdav
      541 htltcalmll ntdlhselfs rrkkrnvghk mtcmefienl aelnegenfp kdvlkalyls
      601 iktaplewav ddseqeapaq dnhtnssear pagfighnpf levpnpncat eykkgyvmrk
      661 ccldpggkrt plgkrgwkmf catlrdlvly lhkdeqgfrk nqlyeslhns irihhslsak
      721 atdytkkqhv frlqtadqae ylfqtgdake lqswvdtlnf vtaslsappl agavgsqkrf
      781 qrpllpvsht kfnlreqltd henrivwlek eleehvanap ekgaksrtva efvekesflq
      841 selkryrtya yllrskmaqy pelvpalvet sigevdepeq nhgshscann ppsssspsrt
      901 kqsalfhktl psspalgant lvspafavpa vasscsprss ptaarnkrls qdrysyrtav
      961 qkgsdlst
//
LOCUS       XP_077505409             277 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115118 [Amblyomma americanum].
ACCESSION   XP_077505409
VERSION     XP_077505409.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649283.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..277
                     /product="uncharacterized protein LOC144115118"
                     /calculated_mol_wt=31348
     Region          23..118
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     CDS             1..277
                     /gene="LOC144115118"
                     /coded_by="XM_077649283.1:191..1024"
                     /db_xref="GeneID:144115118"
ORIGIN      
        1 mfvcytcfrh hdnmsrglfn evliievfkr pllwdvkdnn frnkstkesl weevrdaira
       61 iddtvtveei iarwknlkdt yrrkikeekd gkksgsgata ktawphlkqm eflrdsmetr
      121 rsfcnldavs hvqvasaaae tshascspas pgskvqaspn elesmysvpg lpdepgpscm
      181 aansqevlph rgnkrqkkrr qeveaadrdi qavrsaiaah rpmdasshfl lslrpqleat
      241 pphmlvhlkm ellhichlys qgeypsnlav hkeindh
//
LOCUS       XP_077505410             187 aa            linear   INV 25-NOV-2025
DEFINITION  putative RNA-binding protein 18 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505410
VERSION     XP_077505410.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649284.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="putative RNA-binding protein 18 isoform X1"
                     /calculated_mol_wt=21043
     Region          20..99
                     /region_name="RRM_RBM18"
                     /note="RNA recognition motif (RRM) found in eukaryotic
                     RNA-binding protein 18 and similar proteins; cd12355"
                     /db_xref="CDD:409791"
     CDS             1..187
                     /gene="LOC144115119"
                     /coded_by="XM_077649284.1:61..624"
                     /db_xref="GeneID:144115119"
ORIGIN      
        1 mdtriplpve plphetedkr iwignldsrl tefqlikvlk kygsvkkfdf lfhkagplkg
       61 hprgycfvty etkgqaehal sclhgklvfs ktlvakwann vpnddlsvrr qgsgfgiikk
      121 eqkpvlsvns qihaieaklr amerdkerdl ghcllatpaa psrvvavkpt vaqhqakpyr
      181 qtqrmsr
//
LOCUS       XP_077505411             187 aa            linear   INV 25-NOV-2025
DEFINITION  putative RNA-binding protein 18 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505411
VERSION     XP_077505411.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649285.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="putative RNA-binding protein 18 isoform X2"
                     /calculated_mol_wt=21043
     Region          20..99
                     /region_name="RRM_RBM18"
                     /note="RNA recognition motif (RRM) found in eukaryotic
                     RNA-binding protein 18 and similar proteins; cd12355"
                     /db_xref="CDD:409791"
     CDS             1..187
                     /gene="LOC144115119"
                     /coded_by="XM_077649285.1:112..675"
                     /db_xref="GeneID:144115119"
ORIGIN      
        1 mdtriplpve plphetedkr iwignldsrl tefqlikvlk kygsvkkfdf lfhkagplkg
       61 hprgycfvty etkgqaehal sclhgklvfs ktlvakwann vpnddlsvrr qgsgfgiikk
      121 eqkpvlsvns qihaieaklr amerdkerdl ghcllatpaa psrvvavkpt vaqhqakpyr
      181 qtqrmsr
//
LOCUS       XP_077505412             351 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115120 [Amblyomma americanum].
ACCESSION   XP_077505412
VERSION     XP_077505412.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649286.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..351
                     /product="uncharacterized protein LOC144115120"
                     /calculated_mol_wt=37853
     Region          246..294
                     /region_name="SANT_TRF"
                     /note="Telomere repeat binding factor-like DNA-binding
                     domains of the SANT/myb-like family; cd11660"
                     /db_xref="CDD:212558"
     Site            order(246..248,266,268..269,280,282..284,286..288,
                     290..291)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:212558"
     CDS             1..351
                     /gene="LOC144115120"
                     /coded_by="XM_077649286.1:200..1255"
                     /db_xref="GeneID:144115120"
ORIGIN      
        1 mhrksqpgta gmgspqafme qlcknptlaq lvsrsknkrs cidghtvtai gevlirtgll
       61 qipegvihav ldklvaaces sqhstspevh prplpalqsf sssgqattsr vvsqgtagmg
      121 spqafmeqlc knptlaqlvs rsknkrscid ghtvtaigev lirtgllqip egvihavldk
      181 lvaacessqh stspevhprp lpalqsfsss gqattsrvvs qgtsestrmw mkpsqpaass
      241 vkrprvrfta leedaivygv mkygrgcwkk irddslfngr gptglrdkyr nlekynhlpr
      301 veqrvnakia agvnplkelr aisrqrqqri epsegdvsls dddgiaaptg r
//
LOCUS       XP_077505413             199 aa            linear   INV 25-NOV-2025
DEFINITION  charged multivesicular body protein 1a-like [Amblyomma americanum].
ACCESSION   XP_077505413
VERSION     XP_077505413.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649287.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..199
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..199
                     /product="charged multivesicular body protein 1a-like"
                     /calculated_mol_wt=21768
     Region          13..161
                     /region_name="Snf7"
                     /note="pfam03357"
                     /db_xref="CDD:460896"
     CDS             1..199
                     /gene="LOC144115121"
                     /coded_by="XM_077649287.1:270..869"
                     /db_xref="GeneID:144115121"
ORIGIN      
        1 mddmlfqlrf sckqlerlsk kaekderlqk akikkalqqg nvegariyae nairkknesl
       61 nylrmaskvd avsskiksam amkqvtknms gvvkaldkai nsmdlqkvsa vmekfeqqfe
      121 dldvrssvle damgtatttt apaaqvdqli qqvaeesgle midqlnqagl apadtiaast
      181 grsqavedal tkrlaalre
//
LOCUS       XP_077505414             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115122 [Amblyomma americanum].
ACCESSION   XP_077505414
VERSION     XP_077505414.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649288.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144115122"
                     /calculated_mol_wt=17372
     CDS             1..158
                     /gene="LOC144115122"
                     /coded_by="XM_077649288.1:115..591"
                     /db_xref="GeneID:144115122"
ORIGIN      
        1 mmssseyseg dslsrsdesl steedgedim yqlsvfwpyp kgpdaslrkg pslanlskrp
       61 vlckgpnpqn rrgpdpslrn gpdpknhsgi kwradctgwk gpkadamlvl akpkkrrrrg
      121 akhaassctq erteddesdg gradhqsavr ihrkesev
//
LOCUS       XP_077505415             615 aa            linear   INV 25-NOV-2025
DEFINITION  leukotriene A-4 hydrolase [Amblyomma americanum].
ACCESSION   XP_077505415
VERSION     XP_077505415.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649289.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..615
                     /product="leukotriene A-4 hydrolase"
                     /calculated_mol_wt=69399
     Region          10..455
                     /region_name="M1_LTA4H"
                     /note="Peptidase M1 family including Leukotriene A4
                     hydrolase catalytic domain; cd09599"
                     /db_xref="CDD:341062"
     Site            order(141,143..144,273..277,298,301..302,305,320,324,331,
                     384,389)
                     /site_type="active"
                     /db_xref="CDD:341062"
     Region          514..612
                     /region_name="Leuk-A4-hydro_C"
                     /note="Leukotriene A4 hydrolase, C-terminal; pfam09127"
                     /db_xref="CDD:462686"
     CDS             1..615
                     /gene="LOC144115123"
                     /coded_by="XM_077649289.1:105..1952"
                     /db_xref="GeneID:144115123"
ORIGIN      
        1 mggprltend phsyarpdnc lvtgihldvq vdfqrkiiag faeitckkls ercsclvldt
       61 qdltikgvtq sqggpkldfs laepdpifgs klevqlrpkh faggnctkvc veyetspsss
      121 alqwlrpeqt agklhpylys hcepihcram lpcqdtpsvk mpytasvrap keltvlmsai
      181 regceecgdg atrvtkfiqk vpvpayliai avgevesrkl gprctvwaek kqvdlavidf
      241 edteqmlcsa eslvgpylwd iygllvlpps fpyggmenpc ltfatpslla gdksladvva
      301 heiahswtgn lvtnktfehf wlnegftmfl erkiigrlfg nemrefqalr giedleyave
      361 tmgadnpltc lvpclsgvhp deafssvpye kghtflyyle tllggpdvfd kfllsyieky
      421 kfqsvdtwqw ktylydyfkd ktdilnkvdw nawfhspgmp pvlpsysral vvpceqlcak
      481 wanpatapeh fssndlasfk psqiclllsl llkekplpad rlllltslyk meevgnaeik
      541 frwlrlglra kwepivphvc rflqtygrmk fvcplfrdlh aweekrqltq elfdelkpqm
      601 mhvvtlklsk dlgls
//
LOCUS       XP_077505416             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115125 [Amblyomma americanum].
ACCESSION   XP_077505416
VERSION     XP_077505416.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649290.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144115125"
                     /calculated_mol_wt=14034
     CDS             1..127
                     /gene="LOC144115125"
                     /coded_by="XM_077649290.1:451..834"
                     /db_xref="GeneID:144115125"
ORIGIN      
        1 mhstkwysqp tkrrighgnv llaaailftg csptqslrlv enagicsfsk rtydripkdi
       61 lfpavyqvak ptvslcwesi shfshdtawq nsaippskat pnpahkllvr rvpvttakns
      121 rcvmmvm
//
LOCUS       XP_077505417             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115125 [Amblyomma americanum].
ACCESSION   XP_077505417
VERSION     XP_077505417.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649291.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144115125"
                     /calculated_mol_wt=14034
     CDS             1..127
                     /gene="LOC144115125"
                     /coded_by="XM_077649291.1:652..1035"
                     /db_xref="GeneID:144115125"
ORIGIN      
        1 mhstkwysqp tkrrighgnv llaaailftg csptqslrlv enagicsfsk rtydripkdi
       61 lfpavyqvak ptvslcwesi shfshdtawq nsaippskat pnpahkllvr rvpvttakns
      121 rcvmmvm
//
LOCUS       XP_077505418             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115125 [Amblyomma americanum].
ACCESSION   XP_077505418
VERSION     XP_077505418.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649292.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144115125"
                     /calculated_mol_wt=14034
     CDS             1..127
                     /gene="LOC144115125"
                     /coded_by="XM_077649292.1:258..641"
                     /db_xref="GeneID:144115125"
ORIGIN      
        1 mhstkwysqp tkrrighgnv llaaailftg csptqslrlv enagicsfsk rtydripkdi
       61 lfpavyqvak ptvslcwesi shfshdtawq nsaippskat pnpahkllvr rvpvttakns
      121 rcvmmvm
//
LOCUS       XP_077505419             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115125 [Amblyomma americanum].
ACCESSION   XP_077505419
VERSION     XP_077505419.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649293.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144115125"
                     /calculated_mol_wt=14034
     CDS             1..127
                     /gene="LOC144115125"
                     /coded_by="XM_077649293.1:141..524"
                     /db_xref="GeneID:144115125"
ORIGIN      
        1 mhstkwysqp tkrrighgnv llaaailftg csptqslrlv enagicsfsk rtydripkdi
       61 lfpavyqvak ptvslcwesi shfshdtawq nsaippskat pnpahkllvr rvpvttakns
      121 rcvmmvm
//
LOCUS       XP_077505420             315 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier family 25 member 16-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505420
VERSION     XP_077505420.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649294.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..315
                     /product="solute carrier family 25 member 16-like isoform
                     X1"
                     /calculated_mol_wt=34468
     Region          11..288
                     /region_name="PTZ00169"
                     /note="ADP/ATP transporter on adenylate translocase;
                     Provisional"
                     /db_xref="CDD:240302"
     CDS             1..315
                     /gene="LOC144115126"
                     /coded_by="XM_077649294.1:860..1807"
                     /db_xref="GeneID:144115126"
ORIGIN      
        1 msdktelrsp nfllksffag gvagmcaktt vapldrikil lqghhchykh ygvfsglrgi
       61 vqkeqflgly kgngaqmvri fpyaavqfls feaykrvirn tfentshask fvagscagvt
      121 aavttypldm vrarlafqvn ghhiytgivh vvtsivkteg girglykgls ptvlgmvpya
      181 glsfyvferl kafclevfpn tcgrpypgnt ggivlvipak llcggfagai aqtfsypldv
      241 arrqmqlsmm hpemnkysks lfstltltfr ehgisrglyr gmsvnylrai pmvavsfsty
      301 evakqllgld tglds
//
LOCUS       XP_077505421             291 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier family 25 member 16-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505421
VERSION     XP_077505421.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649295.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..291
                     /product="solute carrier family 25 member 16-like isoform
                     X2"
                     /calculated_mol_wt=31913
     Region          3..264
                     /region_name="PTZ00169"
                     /note="ADP/ATP transporter on adenylate translocase;
                     Provisional"
                     /db_xref="CDD:240302"
     CDS             1..291
                     /gene="LOC144115126"
                     /coded_by="XM_077649295.1:129..1004"
                     /db_xref="GeneID:144115126"
ORIGIN      
        1 mcakttvapl drikillqgh hchykhygvf sglrgivqke qflglykgng aqmvrifpya
       61 avqflsfeay krvirntfen tshaskfvag scagvtaavt typldmvrar lafqvnghhi
      121 ytgivhvvts ivkteggirg lykglsptvl gmvpyaglsf yvferlkafc levfpntcgr
      181 pypgntggiv lvipakllcg gfagaiaqtf sypldvarrq mqlsmmhpem nkyskslfst
      241 ltltfrehgi srglyrgmsv nylraipmva vsfstyevak qllgldtgld s
//
LOCUS       XP_077505422             353 aa            linear   INV 25-NOV-2025
DEFINITION  small ribosomal subunit protein RACK1-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505422
VERSION     XP_077505422.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649296.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..353
                     /product="small ribosomal subunit protein RACK1-like
                     isoform X1"
                     /calculated_mol_wt=39362
     Region          74..346
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cd00200"
                     /db_xref="CDD:238121"
     Site            order(79,85,97..98,116,120,126..127,139..140,157,162,
                     168..169,183,202,207,213..214,226,244,248,254..255,
                     266..267,284,294..295,310..311,335,339,345..346)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          103..139
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          144..180
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          188..223
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          230..265
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          271..314
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          321..345
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..353
                     /gene="LOC144115127"
                     /coded_by="XM_077649296.1:58..1119"
                     /db_xref="GeneID:144115127"
ORIGIN      
        1 mpvrftclfg cspaqafsek vntcfrmsly sprtaplers rqvwstlyva spasplrggl
       61 clpalalrat amhktlilwk ltrddinygi prkrlkghgh fvtdvvmscd gqyalssswd
      121 ktlrlwdlav gsatrrfegh ekdvlstafs adnrqivsgs rdktiklwnt laeckytikd
      181 sqhedwvscv rfsptpgdpt vvscgwdwkv kvwnlancrm raeyshcgyl ntvtvspdgt
      241 lcasggkdck anlwdlekse lkcvrdnqdi inalcyspsr ywlcvatgpa ikiwnledns
      301 qvdelcpevm tpnaktelpq cislawsadg qtlfagytds lirvwqvsig vah
//
LOCUS       XP_077505423             317 aa            linear   INV 25-NOV-2025
DEFINITION  small ribosomal subunit protein RACK1-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505423
VERSION     XP_077505423.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649297.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..317
                     /product="small ribosomal subunit protein RACK1-like
                     isoform X2"
                     /calculated_mol_wt=35506
     Region          7..310
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cd00200"
                     /db_xref="CDD:238121"
     Site            order(14,33,37,43,49,61..62,80,84,90..91,103..104,121,126,
                     132..133,147,166,171,177..178,190,208,212,218..219,
                     230..231,248,258..259,274..275,299,303,309..310)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          18..61
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          67..103
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          108..144
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          152..187
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          194..229
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          235..278
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          285..309
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..317
                     /gene="LOC144115127"
                     /coded_by="XM_077649297.1:222..1175"
                     /db_xref="GeneID:144115127"
ORIGIN      
        1 mteqlhlrgt lrghngwvtq iasnpkfpdt llsssrdktl ilwkltrddi nygiprkrlk
       61 ghghfvtdvv mscdgqyals sswdktlrlw dlavgsatrr feghekdvls tafsadnrqi
      121 vsgsrdktik lwntlaecky tikdsqhedw vscvrfsptp gdptvvscgw dwkvkvwnla
      181 ncrmraeysh cgylntvtvs pdgtlcasgg kdckanlwdl ekselkcvrd nqdiinalcy
      241 spsrywlcva tgpaikiwnl ednsqvdelc pevmtpnakt elpqcislaw sadgqtlfag
      301 ytdslirvwq vsigvah
//
LOCUS       XP_077505424             247 aa            linear   INV 25-NOV-2025
DEFINITION  small ribosomal subunit protein RACK1-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505424
VERSION     XP_077505424.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649298.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..247
                     /product="small ribosomal subunit protein RACK1-like
                     isoform X3"
                     /calculated_mol_wt=27487
     Region          1..33
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <2..240
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cd00200"
                     /db_xref="CDD:238121"
     Region          38..74
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          82..117
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          124..159
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          165..208
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          215..239
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..247
                     /gene="LOC144115127"
                     /coded_by="XM_077649298.1:190..933"
                     /db_xref="GeneID:144115127"
ORIGIN      
        1 mscdgqyals sswdktlrlw dlavgsatrr feghekdvls tafsadnrqi vsgsrdktik
       61 lwntlaecky tikdsqhedw vscvrfsptp gdptvvscgw dwkvkvwnla ncrmraeysh
      121 cgylntvtvs pdgtlcasgg kdckanlwdl ekselkcvrd nqdiinalcy spsrywlcva
      181 tgpaikiwnl ednsqvdelc pevmtpnakt elpqcislaw sadgqtlfag ytdslirvwq
      241 vsigvah
//
LOCUS       XP_077505425             244 aa            linear   INV 25-NOV-2025
DEFINITION  small ribosomal subunit protein RACK1-like isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505425
VERSION     XP_077505425.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649299.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..244
                     /product="small ribosomal subunit protein RACK1-like
                     isoform X4"
                     /calculated_mol_wt=27195
     Region          <17..237
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cd00200"
                     /db_xref="CDD:238121"
     Region          35..71
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          79..114
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          121..156
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          162..205
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          212..236
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..244
                     /gene="LOC144115127"
                     /coded_by="XM_077649299.1:277..1011"
                     /db_xref="GeneID:144115127"
ORIGIN      
        1 mgdanrfqsk vpryapfiis rgsatrrfeg hekdvlstaf sadnrqivsg srdktiklwn
       61 tlaeckytik dsqhedwvsc vrfsptpgdp tvvscgwdwk vkvwnlancr mraeyshcgy
      121 lntvtvspdg tlcasggkdc kanlwdleks elkcvrdnqd iinalcysps rywlcvatgp
      181 aikiwnledn sqvdelcpev mtpnaktelp qcislawsad gqtlfagytd slirvwqvsi
      241 gvah
//
LOCUS       XP_077505426             488 aa            linear   INV 25-NOV-2025
DEFINITION  cdc7 kinase [Amblyomma americanum].
ACCESSION   XP_077505426
VERSION     XP_077505426.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649300.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..488
                     /product="cdc7 kinase"
                     /calculated_mol_wt=54042
     Region          66..485
                     /region_name="STKc_Cdc7"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Cell Division Cycle 7 kinase; cd14019"
                     /db_xref="CDD:270921"
     Site            order(72..76,80,95,97,107,125,141..144,148,150..151,184,
                     186,188..189,191,203,206,331,333..336,338,377)
                     /site_type="active"
                     /db_xref="CDD:270921"
     Site            order(72..75,78,80,95,97,125,141..144,146,188..189,191,
                     203)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270921"
     Site            order(107,150,184,186,206,331,333..336,338,377)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270921"
     Region          <164..>374
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(202..212,326..338)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270921"
     CDS             1..488
                     /gene="Cdc7"
                     /coded_by="XM_077649300.1:345..1811"
                     /db_xref="GeneID:144115128"
ORIGIN      
        1 mavqvlkpfd kfstgqetcr plgdlvkaqq sskassssvk rkssasslsd dnlhlytyip
       61 qieksfildg kigegtfshv ykarlksdpm qtefaikfli ptshpsrian elrclrdmgg
      121 sgnvmdvkmc flhkghvaiv mpyfphekfp dfvcklsvae vqeymrqlci alkrvhsfni
      181 ihrdikpsnf lysreckrfs lvdfglaqrl sdessgltsk tpgppsnslp ppqtaptrkr
      241 trtpdqenqt aqvkrqcllt hmgpplhtln mhnvteadaa tkkpqsvaaa sakpvpkgta
      301 aparrgrqkl cgcfgknevc siclsrpgei apragtpgfr apevllkqrn qttavdmwsa
      361 gviflsllsg rypffraqdd ltalaeiiav igsapvrmaa ekmgkwltls pekpaldlrt
      421 lcerlrgrae akvrktaggk dkqifryhes wlhvpdsayd llsklldpdp mtrltaedal
      481 mhdflkep
//
LOCUS       XP_077505427             488 aa            linear   INV 25-NOV-2025
DEFINITION  cdc7 kinase [Amblyomma americanum].
ACCESSION   XP_077505427
VERSION     XP_077505427.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649301.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..488
                     /product="cdc7 kinase"
                     /calculated_mol_wt=54042
     Region          66..485
                     /region_name="STKc_Cdc7"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Cell Division Cycle 7 kinase; cd14019"
                     /db_xref="CDD:270921"
     Site            order(72..76,80,95,97,107,125,141..144,148,150..151,184,
                     186,188..189,191,203,206,331,333..336,338,377)
                     /site_type="active"
                     /db_xref="CDD:270921"
     Site            order(72..75,78,80,95,97,125,141..144,146,188..189,191,
                     203)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270921"
     Site            order(107,150,184,186,206,331,333..336,338,377)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270921"
     Region          <164..>374
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(202..212,326..338)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270921"
     CDS             1..488
                     /gene="Cdc7"
                     /coded_by="XM_077649301.1:101..1567"
                     /db_xref="GeneID:144115128"
ORIGIN      
        1 mavqvlkpfd kfstgqetcr plgdlvkaqq sskassssvk rkssasslsd dnlhlytyip
       61 qieksfildg kigegtfshv ykarlksdpm qtefaikfli ptshpsrian elrclrdmgg
      121 sgnvmdvkmc flhkghvaiv mpyfphekfp dfvcklsvae vqeymrqlci alkrvhsfni
      181 ihrdikpsnf lysreckrfs lvdfglaqrl sdessgltsk tpgppsnslp ppqtaptrkr
      241 trtpdqenqt aqvkrqcllt hmgpplhtln mhnvteadaa tkkpqsvaaa sakpvpkgta
      301 aparrgrqkl cgcfgknevc siclsrpgei apragtpgfr apevllkqrn qttavdmwsa
      361 gviflsllsg rypffraqdd ltalaeiiav igsapvrmaa ekmgkwltls pekpaldlrt
      421 lcerlrgrae akvrktaggk dkqifryhes wlhvpdsayd llsklldpdp mtrltaedal
      481 mhdflkep
//
LOCUS       XP_077505428             269 aa            linear   INV 25-NOV-2025
DEFINITION  ribosomal protein L7A [Amblyomma americanum].
ACCESSION   XP_077505428
VERSION     XP_077505428.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649302.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..269
                     /product="ribosomal protein L7A"
                     /calculated_mol_wt=30818
     Region          19..268
                     /region_name="SNU13"
                     /note="U4/U6.U5 small nuclear ribonucleoprotein SNU13;
                     cl42391"
                     /db_xref="CDD:455736"
     Site            order(140..145,148,152,164..165,169,190,197..202)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:411042"
     Site            order(144,147..148,151,166,168..169,171..172,175)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:411042"
     CDS             1..269
                     /gene="RpL7A"
                     /coded_by="XM_077649302.1:142..951"
                     /db_xref="GeneID:144115130"
ORIGIN      
        1 mvqrkikgkk gkkkiaaapl svrkteqkkv tnplfekrpr nfsigqdiqp krdlsrfvkw
       61 pkyvriqrqr ailyqrikvp ppinqftqcl drqtatnlfk lldkykpesk aakkqrllqr
      121 aeqkartred vptkrfpvvr qgantvtslv eqkkaqlvvi andvdpvelv lflpalcrkm
      181 gvpycivknk srlgrvvrrk tcsclalaqt npedrsnlsk lveairtnfn eryeeirkhw
      241 gggimgnksa arvaklerar akelaqkva
//
LOCUS       XP_077505429            1187 aa            linear   INV 25-NOV-2025
DEFINITION  putative phosphorylase b kinase regulatory subunit alpha isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077505429
VERSION     XP_077505429.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649303.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (DAPXPI010024294.1) annotated
            using gene prediction method: Gnomon, supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 454 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..1187
                     /product="putative phosphorylase b kinase regulatory
                     subunit alpha isoform X1"
                     /calculated_mol_wt=134019
     Region          8..>437
                     /region_name="Glyco_hydro_15"
                     /note="Glycosyl hydrolases family 15; pfam00723"
                     /db_xref="CDD:395586"
     Region          <799..915
                     /region_name="Bac_rhamnosid6H"
                     /note="Bacterial alpha-L-rhamnosidase 6 hairpin
                     glycosidase domain; cl46875"
                     /db_xref="CDD:481215"
     Region          926..>1112
                     /region_name="KPBB_C"
                     /note="Phosphorylase b kinase C-terminal domain;
                     pfam19292"
                     /db_xref="CDD:437124"
     CDS             1..1187
                     /gene="LOC144115131"
                     /coded_by="XM_077649303.1:1..3564"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 454 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144115131"
ORIGIN      
        1 mrsrrnsgvr ldyyqrlvcr tildhqdpvt glipshkqdd hawvrdnvys ilsvwalsma
       61 yrknadlded raktyeleqg cikmmrglll ammrqkdkve kfkqtqnpad slhakyssqt
      121 tatvvgdrew ghlqldatsl yllilaqmta sglqivfnld evtfiqnlvf yieatycipd
      181 ygiwergdkt nhglpelnss sigmakaalq alddldlfgg kggpfsvihv ladeaqkcna
      241 vlqsmlpres nskevdaall svigypafav ddpeliaqtr ksilqklkgk ygckrflrdg
      301 yktakedpsr lyyeswelqh feniecewpl flcylvidac frgerlevee ysdaleqvll
      361 rtedglklvp emyavpadkv eaeyknphsq prvhvgmtpf mwaqslfiig rllqegfivp
      421 geldplnrrl asekkpdvvv qvvvlaedhi iqeklmhhei fvqtiqdvpd fevlparvlg
      481 hiytylgrst klglsgrqsr dvgilstskm ylfkdsllvf tpqfadqhrf ylasdtdlli
      541 dtfkteiayl ksawrmlgrp tvilpvsqdl lhngkipgev iatikklksg yttgtrillg
      601 tleefvstsc iaslsfvgnq eeghperipa elreylnkef srkdvrrdsi flrrqsiigp
      661 pkspsafgsr gklgvkglvk rsrsihvenf lsydqptstk peellpalse gsyggvgdtg
      721 spaydtdeee lcemvdqpss atilglsllq ldkvsekell dlleesdsle ehgdilhclv
      781 lnkglkwdte lgdgnrvitv rhllkelyek aykehkwglv rhvagllgkk vedlakavtd
      841 llvrqkqvtv gmppmneyti trpqpakqlr iiiykahgad ksaamltqel lvylsmfirt
      901 epelfqemlr lrvgliiqvm vselgrslqc sadeaseyll nmrpyemkml lrqilsgkef
      961 vicnssdgvv svqgskmnkh alqhtmrdsi vtstkkqdea fdrqgqwirr rrldgalnrv
     1021 pmgfyprawk llercvglsi egkvlsqhlt remtqgelkf alefehvlnc ipqpeyrqlm
     1081 veaimvltll aehnvvtfin kvisidglvh haykvfledq klcngdatlc catdgvpkns
     1141 cnkvanlcqh fydsapsgcy gtmtylikav aqtvedlpnt nldctis
//
LOCUS       XP_077505430            1132 aa            linear   INV 25-NOV-2025
DEFINITION  putative phosphorylase b kinase regulatory subunit alpha isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077505430
VERSION     XP_077505430.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649304.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (DAPXPI010024294.1) annotated
            using gene prediction method: Gnomon, supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 454 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..1132
                     /product="putative phosphorylase b kinase regulatory
                     subunit alpha isoform X2"
                     /calculated_mol_wt=128537
     Region          8..>437
                     /region_name="Glyco_hydro_15"
                     /note="Glycosyl hydrolases family 15; pfam00723"
                     /db_xref="CDD:395586"
     Region          <799..915
                     /region_name="Bac_rhamnosid6H"
                     /note="Bacterial alpha-L-rhamnosidase 6 hairpin
                     glycosidase domain; cl46875"
                     /db_xref="CDD:481215"
     Region          926..>1112
                     /region_name="KPBB_C"
                     /note="Phosphorylase b kinase C-terminal domain;
                     pfam19292"
                     /db_xref="CDD:437124"
     CDS             1..1132
                     /gene="LOC144115131"
                     /coded_by="XM_077649304.1:1..3399"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 454 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144115131"
ORIGIN      
        1 mrsrrnsgvr ldyyqrlvcr tildhqdpvt glipshkqdd hawvrdnvys ilsvwalsma
       61 yrknadlded raktyeleqg cikmmrglll ammrqkdkve kfkqtqnpad slhakyssqt
      121 tatvvgdrew ghlqldatsl yllilaqmta sglqivfnld evtfiqnlvf yieatycipd
      181 ygiwergdkt nhglpelnss sigmakaalq alddldlfgg kggpfsvihv ladeaqkcna
      241 vlqsmlpres nskevdaall svigypafav ddpeliaqtr ksilqklkgk ygckrflrdg
      301 yktakedpsr lyyeswelqh feniecewpl flcylvidac frgerlevee ysdaleqvll
      361 rtedglklvp emyavpadkv eaeyknphsq prvhvgmtpf mwaqslfiig rllqegfivp
      421 geldplnrrl asekkpdvvv qvvvlaedhi iqeklmhhei fvqtiqdvpd fevlparvlg
      481 hiytylgrst klglsgrqsr dvgilstskm ylfkdsllvf tpqfadqhrf ylasdtdlli
      541 dtfkteiayl ksawrmlgrp tvilpvsqdl lhngkipgev iatikklksg yttgtrillg
      601 tleefvstsc iaslsfvgnq eeghperipa elreylnkef srkdvrrdsi flrrqsiigp
      661 pkspsafgsr gklgvkglvk rsrsihvenf lsydqptstk peellpalse gsyggvgdtg
      721 spaydtdeee lcemvdqpss atilglsllq ldkvsekell dlleesdsle ehgdilhclv
      781 lnkglkwdte lgdgnrvitv rhllkelyek aykehkwglv rhvagllgkk vedlakavtd
      841 llvrqkqvtv gmppmneyti trpqpakqlr iiiykahgad ksaamltqel lvylsmfirt
      901 epelfqemlr lrvgliiqvm vselgrslqc sadeaseyll nmrpyemkml lrqilsgkef
      961 vicnssdgvv svqgskmnkh alqhtmrdsi vtstkkqdea fdrqgqwirr rrldgalnrv
     1021 pmgfyprawk llercvglsi egkvlsqhlt remtqgelkf alefehvlnc ipqpeyrqlm
     1081 veaimvltll aehnvvtfin kvisidglvh haykvfledq mstrerrelf yh
//
LOCUS       XP_077505431             301 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115132 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505431
VERSION     XP_077505431.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649305.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..301
                     /product="uncharacterized protein LOC144115132 isoform X1"
                     /calculated_mol_wt=32327
     CDS             1..301
                     /gene="LOC144115132"
                     /coded_by="XM_077649305.1:433..1338"
                     /db_xref="GeneID:144115132"
ORIGIN      
        1 mcitmmrwpf pgsllqamvv lilaqaahah csvlpptlrl lplhishpgn iqgavdgpfs
       61 llmcitmmrw pfpgsllqam vvlilaqaah ahcsvlpptl rllplhishp gniqgavdgp
      121 fsllmcitmm rwpfpgsllq amvvlilaqa ahahcsvlpp tlrllplhis hpgniqgavd
      181 gpfsllmcit mmrwpfpgsl lqamvvlila qaahahcsvl pptlrllplh ishpgniqga
      241 vdgpfsllmc itmmrwpfpg sllqamvvli laqaahahcs vlpptlrllp lhishpengp
      301 s
//
LOCUS       XP_077505433             301 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115132 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505433
VERSION     XP_077505433.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649307.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..301
                     /product="uncharacterized protein LOC144115132 isoform X1"
                     /calculated_mol_wt=32327
     CDS             1..301
                     /gene="LOC144115132"
                     /coded_by="XM_077649307.1:109..1014"
                     /db_xref="GeneID:144115132"
ORIGIN      
        1 mcitmmrwpf pgsllqamvv lilaqaahah csvlpptlrl lplhishpgn iqgavdgpfs
       61 llmcitmmrw pfpgsllqam vvlilaqaah ahcsvlpptl rllplhishp gniqgavdgp
      121 fsllmcitmm rwpfpgsllq amvvlilaqa ahahcsvlpp tlrllplhis hpgniqgavd
      181 gpfsllmcit mmrwpfpgsl lqamvvlila qaahahcsvl pptlrllplh ishpgniqga
      241 vdgpfsllmc itmmrwpfpg sllqamvvli laqaahahcs vlpptlrllp lhishpengp
      301 s
//
LOCUS       XP_077505434             239 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115132 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505434
VERSION     XP_077505434.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649308.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..239
                     /product="uncharacterized protein LOC144115132 isoform X2"
                     /calculated_mol_wt=25662
     CDS             1..239
                     /gene="LOC144115132"
                     /coded_by="XM_077649308.1:897..1616"
                     /db_xref="GeneID:144115132"
ORIGIN      
        1 mcitmmrwpf pgsllqamvv lilaqaahah csvlpptlrl lplhishpgn iqgavdgpfs
       61 llmcitmmrw pfpgsllqam vvlilaqaah ahcsvlpptl rllplhishp gniqgavdgp
      121 fsllmcitmm rwpfpgsllq amvvlilaqa ahahcsvlpp tlrllplhis hpgniqgavd
      181 gpfsllmcit mmrwpfpgsl lqamvvlila qaahahcsvl pptlrllplh ishpengps
//
LOCUS       XP_077505435             239 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115132 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505435
VERSION     XP_077505435.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649309.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..239
                     /product="uncharacterized protein LOC144115132 isoform X2"
                     /calculated_mol_wt=25662
     CDS             1..239
                     /gene="LOC144115132"
                     /coded_by="XM_077649309.1:133..852"
                     /db_xref="GeneID:144115132"
ORIGIN      
        1 mcitmmrwpf pgsllqamvv lilaqaahah csvlpptlrl lplhishpgn iqgavdgpfs
       61 llmcitmmrw pfpgsllqam vvlilaqaah ahcsvlpptl rllplhishp gniqgavdgp
      121 fsllmcitmm rwpfpgsllq amvvlilaqa ahahcsvlpp tlrllplhis hpgniqgavd
      181 gpfsllmcit mmrwpfpgsl lqamvvlila qaahahcsvl pptlrllplh ishpengps
//
LOCUS       XP_077505436             629 aa            linear   INV 25-NOV-2025
DEFINITION  protein NYNRIN-like [Amblyomma americanum].
ACCESSION   XP_077505436
VERSION     XP_077505436.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649310.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..629
                     /product="protein NYNRIN-like"
                     /calculated_mol_wt=69644
     Region          343..399
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          <403..529
                     /region_name="transpos_IS481"
                     /note="IS481 family transposase; NF033577"
                     /db_xref="CDD:468094"
     CDS             1..629
                     /gene="LOC144115133"
                     /coded_by="XM_077649310.1:123..2012"
                     /db_xref="GeneID:144115133"
ORIGIN      
        1 mvtfirekrs tdlkelvqra decvmargti nfgrrgsktd slvagqmteq prgpgtrarc
       61 yicsrpghia aqcranrgpp vqknrtpsts gnvstciedg ylelkggqrv pvvsmtalsg
      121 apdlpvvegl isgrkgrvlr dtgcnmvivn rsfvkdeemt gdyrivylvd rsakflpear
      181 ihidtpyyrg evtagcmeep lfdlvlgnie gvrrpgapdt ewkpqnseet eapprehhds
      241 cdkcpdqvtm ptaavttrqq aarqsqggfr klrapatlpd vspdkmrqdq redssllvcf
      301 eadkqrkrtr ckggssftfg lrdgllyrey vpgtgakttq lvvpqkhrek vlqlahgglm
      361 aghlgrkktt driladffwp glqgdvsrfv ascdvcqkte argavrkvpl ervplvdtpf
      421 skvavdivgp lkpttrrgnr yiltvidyat rfpeavalps ietesvaeal lhifarvgvp
      481 eemlsdrgsn ftselmaevg rllslrlktt tpyhpmangl vekmngtlkk mlrrmcaeqp
      541 kdwdrfiepl lfayrevaqa stgfspfell ygrnvrgpla ilrelwtgsr ieeevktaye
      601 yvitsmegvg pdkeelsgla atldkpgtp
//
LOCUS       XP_077505437             615 aa            linear   INV 25-NOV-2025
DEFINITION  G-protein-signaling modulator 2-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505437
VERSION     XP_077505437.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649311.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..615
                     /product="G-protein-signaling modulator 2-like isoform X1"
                     /calculated_mol_wt=67693
     Region          8..>140
                     /region_name="Spy"
                     /note="Predicted O-linked N-acetylglucosamine transferase,
                     SPINDLY family [Posttranslational modification, protein
                     turnover, chaperones]; COG3914"
                     /db_xref="CDD:443119"
     Region          9..34
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          46..78
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          83..365
                     /region_name="TPR"
                     /note="Tetratricopeptide (TPR) repeat [General function
                     prediction only]; COG0457"
                     /db_xref="CDD:440225"
     Region          84..112
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          124..178
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(185,188..189,192..193,195,225,228..229,232..233,
                     235..236,265,268..269,272..273,276)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          185..212
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          217..253
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          264..292
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          408..428
                     /region_name="GoLoco"
                     /note="GoLoco motif; pfam02188"
                     /db_xref="CDD:460481"
     Region          462..483
                     /region_name="GoLoco"
                     /note="GoLoco motif; pfam02188"
                     /db_xref="CDD:460481"
     Region          513..533
                     /region_name="GoLoco"
                     /note="GoLoco motif; pfam02188"
                     /db_xref="CDD:460481"
     Region          558..579
                     /region_name="GoLoco"
                     /note="GoLoco motif; pfam02188"
                     /db_xref="CDD:460481"
     CDS             1..615
                     /gene="LOC144115134"
                     /coded_by="XM_077649311.1:271..2118"
                     /db_xref="GeneID:144115134"
ORIGIN      
        1 messtclela legerlckag dfhsgveyls aainvgtdds rvlsavysql gnasfylgdy
       61 nkalqyhlld lntakaagdl lgeakasgnl gntlkmmgkf deavancerh lsisrekgdr
      121 igearalynl gnvyhakgkh iarasrqepg elpsdvqdcl rkaidcyeen lklmvalgdr
      181 saqgraygnl gntyyllgnf kkaiayheqr lklakefgdk aaqrransnl gnahiflgef
      241 gvaaehytkt yhlakelgdr aveaqacysl gntytllrdf eraieyhlrh lqiaqeltdr
      301 vgegraywsl cnahcslgdh kealhyavqh lhvsqelgdk vgqataqmsi adlkqvlegd
      361 aseaqkgamd dvntpqtasn aedrepakqe tptdaqkqqr sgsdmdqdaf fellsefqsk
      421 rmddqrcsit plenkenvlp ngsppypspt ssgaedsasv rdelldlivg mqsrrmdeqr
      481 tslpqlpglr rnnhnnnslh hhhrmsvgsv peddffdmiv rcqgsrledq rssmptlqra
      541 psapvvpssr saptvpdedf fslilrvqag riedqrtsfr ppdaapttss apvraslkgs
      601 lksalrgsrh skskq
//
LOCUS       XP_077505438             520 aa            linear   INV 25-NOV-2025
DEFINITION  G-protein-signaling modulator 2-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505438
VERSION     XP_077505438.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649312.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..520
                     /product="G-protein-signaling modulator 2-like isoform X2"
                     /calculated_mol_wt=57680
     Region          28..273
                     /region_name="TPR"
                     /note="Tetratricopeptide (TPR) repeat [General function
                     prediction only]; COG0457"
                     /db_xref="CDD:440225"
     Region          29..83
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(90,93..94,97..98,100,130,133..134,137..138,140..141,
                     170,173..174,177..178,181)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          90..117
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          122..158
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          169..197
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          313..333
                     /region_name="GoLoco"
                     /note="GoLoco motif; pfam02188"
                     /db_xref="CDD:460481"
     Region          367..388
                     /region_name="GoLoco"
                     /note="GoLoco motif; pfam02188"
                     /db_xref="CDD:460481"
     Region          418..438
                     /region_name="GoLoco"
                     /note="GoLoco motif; pfam02188"
                     /db_xref="CDD:460481"
     Region          463..484
                     /region_name="GoLoco"
                     /note="GoLoco motif; pfam02188"
                     /db_xref="CDD:460481"
     CDS             1..520
                     /gene="LOC144115134"
                     /coded_by="XM_077649312.1:275..1837"
                     /db_xref="GeneID:144115134"
ORIGIN      
        1 mmgkfdeava ncerhlsisr ekgdrigear alynlgnvyh akgkhiaras rqepgelpsd
       61 vqdclrkaid cyeenlklmv algdrsaqgr aygnlgntyy llgnfkkaia yheqrlklak
      121 efgdkaaqrr ansnlgnahi flgefgvaae hytktyhlak elgdraveaq acyslgntyt
      181 llrdferaie yhlrhlqiaq eltdrvgegr aywslcnahc slgdhkealh yavqhlhvsq
      241 elgdkvgqat aqmsiadlkq vlegdaseaq kgamddvntp qtasnaedre pakqetptda
      301 qkqqrsgsdm dqdaffells efqskrmddq rcsitplenk envlpngspp ypsptssgae
      361 dsasvrdell dlivgmqsrr mdeqrtslpq lpglrrnnhn nnslhhhhrm svgsvpeddf
      421 fdmivrcqgs rledqrssmp tlqrapsapv vpssrsaptv pdedffslil rvqagriedq
      481 rtsfrppdaa pttssapvra slkgslksal rgsrhskskq
//
LOCUS       XP_077505439             227 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115138 [Amblyomma americanum].
ACCESSION   XP_077505439
VERSION     XP_077505439.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649313.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..227
                     /product="uncharacterized protein LOC144115138"
                     /calculated_mol_wt=25245
     Region          43..196
                     /region_name="TesA"
                     /note="Lysophospholipase L1 or related esterase. Includes
                     spore coat protein LipC/YcsK [Cell cycle control, cell
                     division, chromosome partitioning, Lipid transport and
                     metabolism]; COG2755"
                     /db_xref="CDD:442045"
     CDS             1..227
                     /gene="LOC144115138"
                     /coded_by="XM_077649313.1:672..1355"
                     /db_xref="GeneID:144115138"
ORIGIN      
        1 mrkmkevkag metrdnsvqr qesqlersgg qqmelgserl grrrvlvvgd snvarveggv
       61 ltavkadrrv qveaqsgkcm vdamakarev vvgsmdgehl viihaglndv lqgrsqnlet
      121 qlevgmrrlr easesvhvtm ctipevqrqa setewrvvea nrvirllsrr lrygvmevir
      181 evyevrphpf aqddihydga tgkrvgsrig cqakaflgvp raltesv
//
LOCUS       XP_077505440             177 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115139 [Amblyomma americanum].
ACCESSION   XP_077505440
VERSION     XP_077505440.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649314.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..177
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..177
                     /product="uncharacterized protein LOC144115139"
                     /calculated_mol_wt=19027
     CDS             1..177
                     /gene="LOC144115139"
                     /coded_by="XM_077649314.1:1..534"
                     /db_xref="GeneID:144115139"
ORIGIN      
        1 mcitmmrwpf pgsllqamvv lilaqaahah csvlpptlrl lplhishpgn iqgavdgpfs
       61 llmcitmmrw pfpgsllqam vvlilaqaah ahcsvlpptl rllplhishp gniqgavdgp
      121 fsllmcitmm wwpfpgsllq amvvlilaqa ahahcsvlpp tlrllplhis hpengps
//
LOCUS       XP_077505442             166 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115140 [Amblyomma americanum].
ACCESSION   XP_077505442
VERSION     XP_077505442.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649316.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..166
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..166
                     /product="uncharacterized protein LOC144115140"
                     /calculated_mol_wt=17821
     Region          64..85
                     /region_name="GoLoco"
                     /note="GoLoco motif; pfam02188"
                     /db_xref="CDD:460481"
     Region          114..134
                     /region_name="GoLoco"
                     /note="GoLoco motif; pfam02188"
                     /db_xref="CDD:460481"
     CDS             1..166
                     /gene="LOC144115140"
                     /coded_by="XM_077649316.1:16..516"
                     /db_xref="GeneID:144115140"
ORIGIN      
        1 maasraenvf aadckavgpp vaapfcsrvg ghaggwtwps evdaflgaaa ttsegaedna
       61 sardelldli vgmqsrrmde qrtslpqlpg lrrnnhnnsl hhhhrtsvgs vpeddffdmi
      121 vwcqgsrled qrssmptlqr apsapvvpsc drpprcptrt srssca
//
LOCUS       XP_077505443            1752 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin specific peptidase echinus [Amblyomma americanum].
ACCESSION   XP_077505443
VERSION     XP_077505443.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649317.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1752
                     /product="ubiquitin specific peptidase echinus"
                     /calculated_mol_wt=194179
     Region          32..329
                     /region_name="UCH"
                     /note="Ubiquitin carboxyl-terminal hydrolase; pfam00443"
                     /db_xref="CDD:425685"
     Region          916..>1185
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <1037..>1141
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          <1093..1168
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..1752
                     /gene="ec"
                     /coded_by="XM_077649317.1:586..5844"
                     /db_xref="GeneID:144115142"
ORIGIN      
        1 mapaknasss aaslahsmmm prctmsitrt kgllnmpgqn ncflnsavqv lwhldifrrs
       61 frdlsghicm tdscifcalk elfaqfqysq esalppdalr ralaetffdq rrfqlgfmdd
      121 aaecfenilq rihfhiasqe vedmcsvghc iphqkfamtl veqtvchicg asseplpftq
      181 mvhyvsasal clqasivhsk eqkpasfgel lrraggmgdi rdcpsscgak iqicktlmnr
      241 pdivslglvw dserpsvehi vdvldsigmt vnmdemfhkv vdsrwastvt hnlvglvtyy
      301 gkhystfffh tklcvwiyfd datvreigph wqqvvdkchr ghfqplllly anpngtpvst
      361 asaptsviqy msehkkkpmd lkrssekhry gqqsvkkneq isgavniand elpwgrgsyl
      421 sskkaismgg rsavqeplpv ldmafghcak prartysdts imserpfdpt gsgsdleahh
      481 aidsmrrmtk qigrdncldg dlnstyisrq avesvlslqh lqrqrtfnga nttvvpgsrn
      541 sssslesfen slhmrekasd cvdgacrrrd sgnwsgdrns assasstsld gsyyctsgvr
      601 racgspgrav nnfskheysn igmlvdkgyd syslsstdsy psagmpgspa kadprlqqip
      661 eavqisnrle raihhsllqg sdsasdsvfd dvftggkgee esqdcdklcs qaevclkmsy
      721 ekeqagdlkm aiffsdsaaa karaaldapy gnaqsliaak mkhsmcvmrs snlskrlkea
      781 eerrrgkesn gyeahhsrqg srdsthsrht rqgsrdsact vidlsepvkn ieiyatlpkk
      841 gskkkngalf aqsslrlkdd vevykdflvd qwqsssqddl aikmmsdgey tlnpktkmrn
      901 gmgslgkang tkmrdaefsd ysseweqskk pslhrtlsnp tgmknhdade aasvsasmkk
      961 qhrirrklmg gfmrrknrsl pdlregkdgp qtesralddt vifdaknerv mkhessneee
     1021 tredhsatik rrgfhqphra fvsrghaqrp lltkvdppqv egvtssttlh qrstaceqqe
     1081 dspeqaarwl qlrqqpselp qsrqqsselp qprqqpselp qprqqpselp qprqqpselp
     1141 qlrhqpcelf gtndppsfsd aqmpspvpth ssssfsmnqs aisepncflk elhikrtqil
     1201 kslpkaadav rhlgnetstq lksekhlssa ssgnnwlyel qskqmamlkk geqidgarak
     1261 dglpsgkeep lrqhlvlsap qvtnkyaypa dsqghgvqah ngpmqnseee rcenaeeqdd
     1321 ikpksvkdla lkfekilrpa mldpevsnar eepplsiake lplavnkstp tqagnlaaar
     1381 dpgcesqtvn mssslmrter mstptslqss pdsaplnrtf gsahflqpas vqchragntt
     1441 sqqvasviqs cqpynvaiac sgssylpvyq tptspptpct gnsahhhasy nrlfvqqpsi
     1501 qstspassqm hgtatydqrs shfksppvqq qygkafqytr spdpvearsf vaarvpsasp
     1561 avtetllhsm aptnsdsskr ptrppdyeta lqrlemikeq qrtaspdsfq fpakappdgp
     1621 hdqqlpqpdv elgkkrktal kkcvtfsdev vlvacaeeee ddyvpnplle rvyrqhaakq
     1681 dckechepsq fedinsvhss dscdsvchvd ailkphdssq vpcnlchkkl vspptvycpd
     1741 cafymsrfqk rt
//
LOCUS       XP_077505444            1752 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin specific peptidase echinus [Amblyomma americanum].
ACCESSION   XP_077505444
VERSION     XP_077505444.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649318.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1752
                     /product="ubiquitin specific peptidase echinus"
                     /calculated_mol_wt=194179
     Region          32..329
                     /region_name="UCH"
                     /note="Ubiquitin carboxyl-terminal hydrolase; pfam00443"
                     /db_xref="CDD:425685"
     Region          916..>1185
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <1037..>1141
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          <1093..1168
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..1752
                     /gene="ec"
                     /coded_by="XM_077649318.1:178..5436"
                     /db_xref="GeneID:144115142"
ORIGIN      
        1 mapaknasss aaslahsmmm prctmsitrt kgllnmpgqn ncflnsavqv lwhldifrrs
       61 frdlsghicm tdscifcalk elfaqfqysq esalppdalr ralaetffdq rrfqlgfmdd
      121 aaecfenilq rihfhiasqe vedmcsvghc iphqkfamtl veqtvchicg asseplpftq
      181 mvhyvsasal clqasivhsk eqkpasfgel lrraggmgdi rdcpsscgak iqicktlmnr
      241 pdivslglvw dserpsvehi vdvldsigmt vnmdemfhkv vdsrwastvt hnlvglvtyy
      301 gkhystfffh tklcvwiyfd datvreigph wqqvvdkchr ghfqplllly anpngtpvst
      361 asaptsviqy msehkkkpmd lkrssekhry gqqsvkkneq isgavniand elpwgrgsyl
      421 sskkaismgg rsavqeplpv ldmafghcak prartysdts imserpfdpt gsgsdleahh
      481 aidsmrrmtk qigrdncldg dlnstyisrq avesvlslqh lqrqrtfnga nttvvpgsrn
      541 sssslesfen slhmrekasd cvdgacrrrd sgnwsgdrns assasstsld gsyyctsgvr
      601 racgspgrav nnfskheysn igmlvdkgyd syslsstdsy psagmpgspa kadprlqqip
      661 eavqisnrle raihhsllqg sdsasdsvfd dvftggkgee esqdcdklcs qaevclkmsy
      721 ekeqagdlkm aiffsdsaaa karaaldapy gnaqsliaak mkhsmcvmrs snlskrlkea
      781 eerrrgkesn gyeahhsrqg srdsthsrht rqgsrdsact vidlsepvkn ieiyatlpkk
      841 gskkkngalf aqsslrlkdd vevykdflvd qwqsssqddl aikmmsdgey tlnpktkmrn
      901 gmgslgkang tkmrdaefsd ysseweqskk pslhrtlsnp tgmknhdade aasvsasmkk
      961 qhrirrklmg gfmrrknrsl pdlregkdgp qtesralddt vifdaknerv mkhessneee
     1021 tredhsatik rrgfhqphra fvsrghaqrp lltkvdppqv egvtssttlh qrstaceqqe
     1081 dspeqaarwl qlrqqpselp qsrqqsselp qprqqpselp qprqqpselp qprqqpselp
     1141 qlrhqpcelf gtndppsfsd aqmpspvpth ssssfsmnqs aisepncflk elhikrtqil
     1201 kslpkaadav rhlgnetstq lksekhlssa ssgnnwlyel qskqmamlkk geqidgarak
     1261 dglpsgkeep lrqhlvlsap qvtnkyaypa dsqghgvqah ngpmqnseee rcenaeeqdd
     1321 ikpksvkdla lkfekilrpa mldpevsnar eepplsiake lplavnkstp tqagnlaaar
     1381 dpgcesqtvn mssslmrter mstptslqss pdsaplnrtf gsahflqpas vqchragntt
     1441 sqqvasviqs cqpynvaiac sgssylpvyq tptspptpct gnsahhhasy nrlfvqqpsi
     1501 qstspassqm hgtatydqrs shfksppvqq qygkafqytr spdpvearsf vaarvpsasp
     1561 avtetllhsm aptnsdsskr ptrppdyeta lqrlemikeq qrtaspdsfq fpakappdgp
     1621 hdqqlpqpdv elgkkrktal kkcvtfsdev vlvacaeeee ddyvpnplle rvyrqhaakq
     1681 dckechepsq fedinsvhss dscdsvchvd ailkphdssq vpcnlchkkl vspptvycpd
     1741 cafymsrfqk rt
//
LOCUS       XP_077505445             121 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier organic anion transporter family member 4A1-like
            [Amblyomma americanum].
ACCESSION   XP_077505445
VERSION     XP_077505445.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649319.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..121
                     /product="solute carrier organic anion transporter family
                     member 4A1-like"
                     /calculated_mol_wt=13032
     Region          <23..120
                     /region_name="OATP"
                     /note="Organic Anion Transporter Polypeptide (OATP)
                     family; pfam03137"
                     /db_xref="CDD:460821"
     CDS             1..121
                     /gene="LOC144115143"
                     /coded_by="XM_077649319.1:1..366"
                     /db_xref="GeneID:144115143"
ORIGIN      
        1 malrdvkplr elafhpglgp qvysdcccia tvrekdvlsv qaeltrrqtt ckalapfsmg
       61 lflalfetfl nsapgmsgti rtvgeptkpv alglqwvsvr lfgtilapiv lcsiihrscl
      121 t
//
LOCUS       XP_077505446             785 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077505446
VERSION     XP_077505446.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649320.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..785
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=88206
     Region          101..236
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          345..726
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..785
                     /gene="LOC144115144"
                     /coded_by="XM_077649320.1:133..2490"
                     /db_xref="GeneID:144115144"
ORIGIN      
        1 mrppvmlaal lllvlgtaaa spaesdtrps vpsptttprt vrsrapvrtt tkdpgpdprn
       61 pykyyarkqe klvktlmkti tehvtpqflg disqlnltgd ctyglfkmif gvkqlkpwai
      121 kmldasgkmp eglffgslta fgdmgeclqv rainrrlngk leeyfqgqyc aldirfklpp
      181 kparyspktf lknltdqipl ykhllgdvsv gyfyftpirm alcvpstcsq sdiqtlgsav
      241 gdlaqldvsv pfceklqptq yttlqlisma ilafffclvl lgtfmdvapr vlhswvshgd
      301 vtsyaekdse tnrnvvldvl lsfsaftngk rllstrtaig vleplhgmrv itmawiilgh
      361 tffyksyvls gglfealeyg ksfpfqivln vfpavetfvt msgilvayns lrklekcqgs
      421 fnpfsyflhr ylrltpafll vsllmillpl tgsgpvwnet lqpfakacek rwwtnilyig
      481 swfkreemcl phgwylscdm hyyiiapiif ilmfkrpsvs ftllallgvg siasvgvlty
      541 iwqfppipif vdpdpdhfne fmsfigyrpy thltafcigm atgyflyknk tlhinrvarl
      601 lgwlaaalvn mtvvfgvynw nayeapslsv avayaalsrt awalgiawmv vacatgnggf
      661 inkvlswraf vplsrltylv ylvhpllilw htaylkkpfy ttqffvgyvy lghlfsaycl
      721 sfslsltfes pfillerill gkpirqngyh ieheksdkil ardfsksada httksfengt
      781 sscgv
//
LOCUS       XP_077505447             928 aa            linear   INV 25-NOV-2025
DEFINITION  ecotropic viral integration site 5 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505447
VERSION     XP_077505447.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649321.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..928
                     /product="ecotropic viral integration site 5 isoform X1"
                     /calculated_mol_wt=104621
     Region          224..432
                     /region_name="TBC"
                     /note="Domain in Tre-2, BUB2p, and Cdc16p. Probable
                     Rab-GAPs; smart00164"
                     /db_xref="CDD:214540"
     Region          <467..805
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..928
                     /gene="Evi5"
                     /coded_by="XM_077649321.1:276..3062"
                     /db_xref="GeneID:144115146"
ORIGIN      
        1 mrfldayagp aatepnsrrs alkvadnvaa sapaakkrvw fsaalsamgl ndtnarepil
       61 svdgsshpeg pmseaevkpc slqggdanhn hpntlnlatt ngnstegpsp qalpgqpsvt
      121 satpvdaptp vvstpvskad mellarleeq nrllesdsks lnsltsthsr kssdtsqvsv
      181 asgasqqsta ldddsedawv lwgrmvnewd tyikkknayv keqvrkgipq hfraiawqll
      241 cnapacaare qyaeylkass pcekvirrdi artypeheff rdkdgpgqeg lfnvmkaysl
      301 hdrevgycqg safivgllll qmpeeetftv lvrmmqdyrl reiykpsmae lglcmyqlec
      361 lvqelvpeih mhfqaqsfht smyasswflt lftsclphtl acrvmdlfls egmemifria
      421 iailqfcked ilqldmegml ryfqkempsk cetdpdylin lalqvkynsk kikklekeyt
      481 alkvkeheel ielrrlrten rllrqriehl eqessaladr liqgqvvraq eaednyiikr
      541 elaalrqqel tvqqelqkan nqikelhqaq tqpssldsqt edllkslqde lvavklreae
      601 gqeamrslrq riheleeenk rlreatpdds vatlqeelia vklreaeanl smkelrqkva
      661 dlqaawekhr senphqsdnq stpsrwsgnk sqvnqlqeel mtarlkeada taelrelrqr
      721 vmeletqnqv slnqirrqad eiakyqqmsd kvlekerelq slvqdearki tnlesqmkee
      781 rmnarireve htqiiaemkq kissleiknq elltagqlrg steseevkel qdkvsdlkae
      841 vmqlqimnkr lssalavarl rqpdaqkvgv aaasgplsss ppltlvggls rstslvedsv
      901 teqllqlgle lsvsqhpakq pklsngel
//
LOCUS       XP_077505448            1003 aa            linear   INV 25-NOV-2025
DEFINITION  ecotropic viral integration site 5 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505448
VERSION     XP_077505448.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649322.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1003
                     /product="ecotropic viral integration site 5 isoform X2"
                     /calculated_mol_wt=112463
     Region          61..>273
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     Region          299..507
                     /region_name="TBC"
                     /note="Domain in Tre-2, BUB2p, and Cdc16p. Probable
                     Rab-GAPs; smart00164"
                     /db_xref="CDD:214540"
     Region          <542..880
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1003
                     /gene="Evi5"
                     /coded_by="XM_077649322.1:226..3237"
                     /db_xref="GeneID:144115146"
ORIGIN      
        1 mqrggsektc dsaaggargs plekfrlvsv nalrlaartv ellqeqcaea aaarsfsktr
       61 avtdagasap vaarragpgg eatsapeltt ndaeqrrrfd ssrrpdiaqf sphnvspevl
      121 sslralrvad gepilsvdgs shpegpmsea evkpcslqgg danhnhpntl nlattngnst
      181 egpspqalpg qpsvtsatpv daptpvvstp vskadmella rleeqnrlle sdskslnslt
      241 sthsrkssdt sqvsvasgas qqstalddds edawvlwgrm vnewdtyikk knayvkeqvr
      301 kgipqhfrai awqllcnapa caareqyaey lkasspcekv irrdiartyp eheffrdkdg
      361 pgqeglfnvm kayslhdrev gycqgsafiv gllllqmpee etftvlvrmm qdyrlreiyk
      421 psmaelglcm yqleclvqel vpeihmhfqa qsfhtsmyas swfltlftsc lphtlacrvm
      481 dlflsegmem ifriaiailq fckedilqld megmlryfqk empskcetdp dylinlalqv
      541 kynskkikkl ekeytalkvk eheelielrr lrtenrllrq riehleqess aladrliqgq
      601 vvraqeaedn yiikrelaal rqqeltvqqe lqkannqike lhqaqtqpss ldsqtedllk
      661 slqdelvavk lreaegqeam rslrqrihel eeenkrlrea tpddsvatlq eeliavklre
      721 aeanlsmkel rqkvadlqaa wekhrsenph qsdnqstpsr wsgnksqvnq lqeelmtarl
      781 keadataelr elrqrvmele tqnqvslnqi rrqadeiaky qqmsdkvlek erelqslvqd
      841 earkitnles qmkeermnar irevehtqii aemkqkissl eiknqellta gqlrgstese
      901 evkelqdkvs dlkaevmqlq imnkrlssal avarlrqpda qkvgvaaasg plsssppltl
      961 vgglsrstsl vedsvteqll qlglelsvsq hpakqpklsn gel
//
LOCUS       XP_077505449             857 aa            linear   INV 25-NOV-2025
DEFINITION  ecotropic viral integration site 5 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505449
VERSION     XP_077505449.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649323.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..857
                     /product="ecotropic viral integration site 5 isoform X3"
                     /calculated_mol_wt=97257
     Region          153..361
                     /region_name="TBC"
                     /note="Domain in Tre-2, BUB2p, and Cdc16p. Probable
                     Rab-GAPs; smart00164"
                     /db_xref="CDD:214540"
     Region          <396..734
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..857
                     /gene="Evi5"
                     /coded_by="XM_077649323.1:382..2955"
                     /db_xref="GeneID:144115146"
ORIGIN      
        1 mseaevkpcs lqggdanhnh pntlnlattn gnstegpspq alpgqpsvts atpvdaptpv
       61 vstpvskadm ellarleeqn rllesdsksl nsltsthsrk ssdtsqvsva sgasqqstal
      121 dddsedawvl wgrmvnewdt yikkknayvk eqvrkgipqh fraiawqllc napacaareq
      181 yaeylkassp cekvirrdia rtypeheffr dkdgpgqegl fnvmkayslh drevgycqgs
      241 afivgllllq mpeeetftvl vrmmqdyrlr eiykpsmael glcmyqlecl vqelvpeihm
      301 hfqaqsfhts myasswfltl ftsclphtla crvmdlflse gmemifriai ailqfckedi
      361 lqldmegmlr yfqkempskc etdpdylinl alqvkynskk ikklekeyta lkvkeheeli
      421 elrrlrtenr llrqriehle qessaladrl iqgqvvraqe aednyiikre laalrqqelt
      481 vqqelqkann qikelhqaqt qpssldsqte dllkslqdel vavklreaeg qeamrslrqr
      541 iheleeenkr lreatpddsv atlqeeliav klreaeanls mkelrqkvad lqaawekhrs
      601 enphqsdnqs tpsrwsgnks qvnqlqeelm tarlkeadat aelrelrqrv meletqnqvs
      661 lnqirrqade iakyqqmsdk vlekerelqs lvqdearkit nlesqmkeer mnarireveh
      721 tqiiaemkqk issleiknqe lltagqlrgs teseevkelq dkvsdlkaev mqlqimnkrl
      781 ssalavarlr qpdaqkvgva aasgplsssp pltlvgglsr stslvedsvt eqllqlglel
      841 svsqhpakqp klsngel
//
LOCUS       XP_077505451             566 aa            linear   INV 25-NOV-2025
DEFINITION  PCNA-interacting partner-like [Amblyomma americanum].
ACCESSION   XP_077505451
VERSION     XP_077505451.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649325.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..566
                     /product="PCNA-interacting partner-like"
                     /calculated_mol_wt=64277
     CDS             1..566
                     /gene="LOC144115147"
                     /coded_by="XM_077649325.1:1..1701"
                     /db_xref="GeneID:144115147"
ORIGIN      
        1 msdnenasic flwttynpcv enavfeymke eagskelyil rtetladdls sctvfgflvn
       61 alrkrnafvn rsttvlsvpe hsqaihlcav rvqqrerakf yttsakfdtt sfevadadla
      121 yqrrlsksdv cqfepeclqe ydeflshtnt vnswqilqmf rdalqddstf aaqfansvfl
      181 vygpiteefg kecvellsqg rkirrivaeg hldmsanevy niedvsvsse vadvpepdcs
      241 averwdsitv aymvcitnaf irvllnsnde lalatamasp ivelphdaft rlkylslkkk
      301 tpmcetaish vkqanlggds campenhplk ryfyklenfv kllsdlqntv evvptanaas
      361 viistlvlri tkaakrrglv kervthfkde ltrlarrfqd eegtgnvdda kdtvqgsatl
      421 rilqrlsdfl strriscmre eliynwgnyg tplnipemvk yfrtpesede ddddynvplr
      481 erilknfqsd sekesfsrml aeidkkskrk rrrskqqldh elyippkvya vtdeaplael
      541 ksarkkaakr smladisgtk kkgsnn
//
LOCUS       XP_077505452             492 aa            linear   INV 25-NOV-2025
DEFINITION  brix domain-containing protein peter pan [Amblyomma americanum].
ACCESSION   XP_077505452
VERSION     XP_077505452.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649326.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..492
                     /product="brix domain-containing protein peter pan"
                     /calculated_mol_wt=56048
     Region          33..202
                     /region_name="Brix"
                     /note="The Brix domain is found in a number of eukaryotic
                     proteins; smart00879"
                     /db_xref="CDD:214879"
     CDS             1..492
                     /gene="ppan"
                     /coded_by="XM_077649326.1:133..1611"
                     /db_xref="GeneID:144115148"
ORIGIN      
        1 mgakkrkgra vkaakkgsas vepeeiskap htfvisrgtl gknaselmln frkvmepyta
       61 sklkvrrknv vkdfvsvagl lhvthlvvft ktqkaiymrl arlprgptlt frvenyalar
      121 dvisslkkpi tythqfsshp llvlnnfsge gmhfklmasm fqnmfpsini hkvklntirr
      181 clllnynstd qsidfrhyti kvapcginra vkklvqskvp dlghlrdise yvdqgglses
      241 eaeldgasnq vvlpqkissr gntlnqqssv rmvelgprmk lklvkieegl mtgevmyhal
      301 vqrtpeeika lrerrlklkk lkekrkreqe enkkkkealq kqhgdegsda dveaeseedd
      361 dqewyrhevg edpeeegalp kakvakrksg gmvdtkspap kklkfqqkqh grtgkvpalk
      421 kgkdgageeq egkkpqkrkl sdfevmmqrr kkrrklkekt kekrlrsqkv arlraqqkqd
      481 vpfkkakhkr nr
//
LOCUS       XP_077505453             454 aa            linear   INV 25-NOV-2025
DEFINITION  zinc transporter Slc39a7-like [Amblyomma americanum].
ACCESSION   XP_077505453
VERSION     XP_077505453.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649327.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..454
                     /product="zinc transporter Slc39a7-like"
                     /calculated_mol_wt=48575
     Region          104..451
                     /region_name="Zip"
                     /note="ZIP Zinc transporter; pfam02535"
                     /db_xref="CDD:396884"
     CDS             1..454
                     /gene="LOC144115150"
                     /coded_by="XM_077649327.1:153..1517"
                     /db_xref="GeneID:144115150"
ORIGIN      
        1 mlsasrracv aallialvsa elrlvgaqhh hhhdhdhdhd hdhehvhepa afkysraand
       61 phlatgkeah rhfhghvass sshshasdgp kkkpirsfed taflwgralg stllisvapf
      121 lilffipids rsghesllkv llsfasggll gdaflhliph almphgneel sgaaheshah
      181 shahshshgh dhshgphdms vglwvlagil aflmvekfvr mikgghghsh ehadghdhre
      241 eqagdqtpsg mgeadtgpsg kcdgessgte eakseaaaal vrrkkakles ptkdeksadg
      301 gkrpsdikva aylnlaadft hnftdglaig asyiagntag fistvtillh evpheigdfa
      361 ilvqsgyskr ramcmqlvta vgclsgtlcs liadgvsssa nlwvlpftag gfiyiatvsv
      421 ipellentrl wqsvkeifal llgvsmmall tqfe
//
LOCUS       XP_077505454             399 aa            linear   INV 25-NOV-2025
DEFINITION  torsin-2A-like [Amblyomma americanum].
ACCESSION   XP_077505454
VERSION     XP_077505454.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649328.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..399
                     /product="torsin-2A-like"
                     /calculated_mol_wt=43543
     Region          140..>242
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            174..181
                     /site_type="other"
                     /note="Walker A motif"
                     /db_xref="CDD:99707"
     Site            order(175..182,232)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:99707"
     Site            228..233
                     /site_type="other"
                     /note="Walker B motif"
                     /db_xref="CDD:99707"
     CDS             1..399
                     /gene="LOC144115151"
                     /coded_by="XM_077649328.1:305..1504"
                     /db_xref="GeneID:144115151"
ORIGIN      
        1 mvkkrvshah igheskhast ksnsnsgpkk llaitypghe pkrsltanas rngyahkkat
       61 llseckekrq gsmngylpvk wsvvskkyvk ryiviawtfl qacvtlgclf lvylffhpdy
      121 lyserlqvem ldvtlhqhlv gqdiainrtv aaiarylggg esvaplplvl vyagcsgcgk
      181 tyalsviakh cphtealvas hqvalarrtg nnllhwlqst lssrrpniiv iddvdlapda
      241 aklgpgdqsf saqletvlss wgqlglqtgq aaifvlslss gasvaegfal kqlsdkqnms
      301 ssedveglve ayrymlppwl knaeivpflp ltkadvkecl lrqlaqhtpe frarvlalpg
      361 gvnsllqefr fypaenpifs esgckevpik lalvatdsp
//
LOCUS       XP_077505455             348 aa            linear   INV 25-NOV-2025
DEFINITION  torsin-1A-like [Amblyomma americanum].
ACCESSION   XP_077505455
VERSION     XP_077505455.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649329.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..348
                     /product="torsin-1A-like"
                     /calculated_mol_wt=39440
     Region          70..182
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            order(115..121,126)
                     /site_type="other"
                     /note="Walker A motif"
                     /db_xref="CDD:99707"
     Site            order(116..121,126..127,183,221)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:99707"
     Site            179..184
                     /site_type="other"
                     /note="Walker B motif"
                     /db_xref="CDD:99707"
     CDS             1..348
                     /gene="LOC144115152"
                     /coded_by="XM_077649329.1:312..1358"
                     /db_xref="GeneID:144115152"
ORIGIN      
        1 mkfyreyyvy ifliciwfsl grrceslepv tvstaiavga glgvsvmyag wnalkcktse
       61 ccaapwvtdn vkgldntlkf rlhgqplvhr avvralkdhf qnpspkkalv lsfhgwtggg
      121 knyassmiae alykegmnsk yvslyvankh fphqdevpky rktlqkeiea kvkecgrslf
      181 ifdeidkmpn glidiikpyl dfhekldgvd yrksifvfls ntagdviara tldawkdgid
      241 rskvrlndme kvielgsfne kgglyradiv qknlidvfvp flplerkqiv acirdefkrr
      301 grtasdkmie dianeldyip aeyalysstg ckkihnkvaq hlgfseki
//
LOCUS       XP_077505456             918 aa            linear   INV 25-NOV-2025
DEFINITION  ER degradation enhancer, mannosidase alpha-like 2 [Amblyomma
            americanum].
ACCESSION   XP_077505456
VERSION     XP_077505456.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649330.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..918
                     /product="ER degradation enhancer, mannosidase alpha-like
                     2"
                     /calculated_mol_wt=102769
     Region          40..477
                     /region_name="Glyco_hydro_47"
                     /note="Glycosyl hydrolase family 47; pfam01532"
                     /db_xref="CDD:460241"
     Region          633..757
                     /region_name="PA"
                     /note="Protease-associated (PA) domain. The PA domain is
                     an insert domain in a diverse fraction of proteases. The
                     significance of the PA domain to many of the proteins in
                     which it is inserted is undetermined. It may be a
                     protein-protein interaction domain. At...; cl28883"
                     /db_xref="CDD:333703"
     Site            718..720
                     /site_type="other"
                     /note="PA/protease or protease-like domain interface
                     [polypeptide binding]"
                     /db_xref="CDD:238617"
     CDS             1..918
                     /gene="Edem2"
                     /coded_by="XM_077649330.1:173..2929"
                     /db_xref="GeneID:144115155"
ORIGIN      
        1 meplsrrtva iagllmcvfg tteavtaeer vqlrdqvkem fyhayrayvd naypadelmp
       61 lsckgryrgt eanrgdidda lgnfsltlvd tmdtlvllgd meeferavkf virdvsfdsd
      121 ivvsvfetni rmvggllsgh ilasylqerh glmtwyrgdl llmakelgyr llpafntstg
      181 vphprinlrl glkspklgqv hetctacagt milefaalsr ltgepifeak araamdylwq
      241 qrhrssdlmg tvlnihngdw irrdsgvgag idsyyeyclk ayillgddly lerfnkhyta
      301 vmkyvsqgpm lvdvhmhrpn tnsrnfmdal lafwpglqvl kgdikpaiet hemlyqvmkl
      361 hnflpeaftt dfqvhwgqhp lrpefvesty flfkatgdpy yleagrrvve slqkharvpc
      421 gfaavkdvrt gthedrmdsf vlaetlkyly llfafpedlp ldldhfvftt eahllplsla
      481 rinrthfdmm qsprmmfyap eeefshscpn thflfpksny ahsvrqplkn lvegacptva
      541 sstrrlhasq feaskkehln mvkkmgvfvt lmsdgriqli hnaaqaespe daqegllfmq
      601 emielakqqn qqeqhvrsvt ftlpstkvhm vlqagpaqfg kdlgkpanev saslvlldpl
      661 rgcgelrngh elrgkivime rgecmfveka rkiqafgavg givldnqvgt saqqspvfam
      721 sgdgtddvni plvflftedg qlllnafreh pdlvvtlsdl svissaasdt gseaaegpan
      781 qtspevssqk vstttqterg pssaeaellr kmqilrhlda lgrlhgetne llekeatvrr
      841 mlgkllsqhk lfemdirsll erledltvea karrsrghal ctsplvpvts svnplssaea
      901 fvafitrhhc eyatkasr
//
LOCUS       XP_077505457             306 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115157 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505457
VERSION     XP_077505457.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649331.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..306
                     /product="uncharacterized protein LOC144115157 isoform X1"
                     /calculated_mol_wt=34237
     CDS             1..306
                     /gene="LOC144115157"
                     /coded_by="XM_077649331.1:622..1542"
                     /db_xref="GeneID:144115157"
ORIGIN      
        1 mespvevieg lvdlavtngf ttavaellvh lgvvckykkc peaeqdvlgs flletfenlv
       61 dtmtskhhnn yvhlieelvk csnivncdwl liriiryiel agasdphped ffsvrvfmai
      121 apkmskhyiv pmaqmlvqml dkaclelnga rkekvlrffq ayvdigavtv hnfsgelvll
      181 daeqwrnece hiaaqfpqps lrdllmpyfc ldclkatddl seedlqdilv vlmfgltslp
      241 gqcfsqqlll lvdvtqrmkt selkeelvvl lqeallmkcs edgavlnlvq aalrklgahr
      301 asqadv
//
LOCUS       XP_077505458             306 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115157 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505458
VERSION     XP_077505458.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649332.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..306
                     /product="uncharacterized protein LOC144115157 isoform X1"
                     /calculated_mol_wt=34237
     CDS             1..306
                     /gene="LOC144115157"
                     /coded_by="XM_077649332.1:669..1589"
                     /db_xref="GeneID:144115157"
ORIGIN      
        1 mespvevieg lvdlavtngf ttavaellvh lgvvckykkc peaeqdvlgs flletfenlv
       61 dtmtskhhnn yvhlieelvk csnivncdwl liriiryiel agasdphped ffsvrvfmai
      121 apkmskhyiv pmaqmlvqml dkaclelnga rkekvlrffq ayvdigavtv hnfsgelvll
      181 daeqwrnece hiaaqfpqps lrdllmpyfc ldclkatddl seedlqdilv vlmfgltslp
      241 gqcfsqqlll lvdvtqrmkt selkeelvvl lqeallmkcs edgavlnlvq aalrklgahr
      301 asqadv
//
LOCUS       XP_077505459             306 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115157 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505459
VERSION     XP_077505459.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649333.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..306
                     /product="uncharacterized protein LOC144115157 isoform X1"
                     /calculated_mol_wt=34237
     CDS             1..306
                     /gene="LOC144115157"
                     /coded_by="XM_077649333.1:1185..2105"
                     /db_xref="GeneID:144115157"
ORIGIN      
        1 mespvevieg lvdlavtngf ttavaellvh lgvvckykkc peaeqdvlgs flletfenlv
       61 dtmtskhhnn yvhlieelvk csnivncdwl liriiryiel agasdphped ffsvrvfmai
      121 apkmskhyiv pmaqmlvqml dkaclelnga rkekvlrffq ayvdigavtv hnfsgelvll
      181 daeqwrnece hiaaqfpqps lrdllmpyfc ldclkatddl seedlqdilv vlmfgltslp
      241 gqcfsqqlll lvdvtqrmkt selkeelvvl lqeallmkcs edgavlnlvq aalrklgahr
      301 asqadv
//
LOCUS       XP_077505460             245 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115157 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505460
VERSION     XP_077505460.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649334.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..245
                     /product="uncharacterized protein LOC144115157 isoform X2"
                     /calculated_mol_wt=27171
     CDS             1..245
                     /gene="LOC144115157"
                     /coded_by="XM_077649334.1:622..1359"
                     /db_xref="GeneID:144115157"
ORIGIN      
        1 mespvevieg lvdlavtngf ttavaellvh lgvvckykkc peaeqdvlgs flletfenlv
       61 dtmtskhhnn yvhlieelvk csnivncdwl liriiryiel agasdphped ffsvrvfmai
      121 apkmskhyiv pmaqmlvqml dkaclelnga rkekvlrffq ayvdigavtv hnfsgelvll
      181 daeqwrnece hiaaqfpqps lrdllmpyfc lwlagatsed sattfdvpas lgarptllla
      241 tgapc
//
LOCUS       XP_077505461             222 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115157 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505461
VERSION     XP_077505461.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649335.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..222
                     /product="uncharacterized protein LOC144115157 isoform X3"
                     /calculated_mol_wt=24984
     CDS             1..222
                     /gene="LOC144115157"
                     /coded_by="XM_077649335.1:622..1290"
                     /db_xref="GeneID:144115157"
ORIGIN      
        1 mespvevieg lvdlavtngf ttavaellvh lgvvckykkc peaeqdvlgs flletfenlv
       61 dtmtskhhnn yvhlieelvk csnivncdwl liriiryiel agasdphped ffsvrvfmai
      121 apkmskhyiv pmaqmlvqml dkaclelnga rkekvlrffq ayvdigavtv hnfsgelvll
      181 daeqwrnece hiaaqfpqps lrdllmpyfc lwlagatsee iv
//
LOCUS       XP_077505462            1155 aa            linear   INV 25-NOV-2025
DEFINITION  codanin-1 like protein dlt [Amblyomma americanum].
ACCESSION   XP_077505462
VERSION     XP_077505462.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649336.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1155
                     /product="codanin-1 like protein dlt"
                     /calculated_mol_wt=129363
     Region          760..844
                     /region_name="Codanin-1_C"
                     /note="Codanin-1 C-terminus; pfam15296"
                     /db_xref="CDD:464622"
     CDS             1..1155
                     /gene="dlt"
                     /coded_by="XM_077649336.1:258..3725"
                     /db_xref="GeneID:144115158"
ORIGIN      
        1 mavvldllls ktlsseellq wltdpqyeps lpdlkllrsq rkdfveafin firdqcpliq
       61 qassqarpqq pnaapsrvan cgtartakle salsvksppt vvgrrqctvr vgdcdksalp
      121 aasakcdtsr asgvdisafk sttshrnekr tvfkdrstpa tlgvaarapd iadcaafpav
      181 neavestrpi rritptpvks nwrkltlkdf lppevshecq rkegcrprhg qkeskestrn
      241 qfkrtdalql rlsaapqscs saqrrdkkhr ttapavvsad tnihsteevq adsyivpapd
      301 citerekldd vaavyaalln kglvvnltie lffllklltv keeprlseep hstkllesvh
      361 nciyfaaavl qgilqwlcfl dkptlkllsn vqhiatfapq lyssivslvd cveplsrfws
      421 ssiqgvafds ttdsrkhfas ennfrafrkq rdlfynlfrd wqrhqfcsge kfsaqfphrv
      481 aeildtcsdp cnlshlarlv lgqlmtsccg tlelddqgnf dekflhdlks tspekmekle
      541 erfllpfkvg gpcpspsftg sqiffyefik aatnsiflqh fkdqcvskiv elekdnpfqd
      601 aqqspseelk raliaavyks kllglflgvv eflpysties fpkqyidqqq tvrnlkplpl
      661 nvtglikesv kskqlaatlt wvvnflsmmd piaktliayr dvlwllfsiy kspallqass
      721 aaffarvllg wlfevqsiqs dvlayapndi lgqaeslgsf vdgciiymcc pylgelrcil
      781 lehltgtktk ygefrkitpi sasesdlkaq lknqleenff hihpvsvkkt vefvadrvss
      841 nvakclkkdv qagiahsrdi ishmnwaeie keerqltimk lfqqisekae ktaleqckel
      901 iekllpvllp pdmqgqavkm ccylstksav skvrqwlktn vtekafrqgi saaphqansd
      961 qntsedkdvs shyhaasafn ildrlqdcmr qlqsgrgctc edavkllelc aeslhssqew
     1021 fpfattaleq atfdfvlcla vwcpqactrd vfdvavpmwe cshavkkack mlycarnlhl
     1081 avtsadtrct llkfgelaqv llyfglltva dleeslkkvl dfelpsllle ltvcvlssvl
     1141 elyknsnenl serpd
//
LOCUS       XP_077505463             219 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115159 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505463
VERSION     XP_077505463.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649337.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..219
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..219
                     /product="uncharacterized protein LOC144115159 isoform X1"
                     /calculated_mol_wt=24109
     CDS             1..219
                     /gene="LOC144115159"
                     /coded_by="XM_077649337.1:101..760"
                     /db_xref="GeneID:144115159"
ORIGIN      
        1 msrrvmtlhg spacfgglls rvssersrvs qvskvhskma tckrhkerlh esismpqlgl
       61 aprlalvlfl igkkprlqea vilapradpl vvvpkyrlnl sglftpnwsd apdqgqgshg
      121 cwcayrplaa tqapwsrrsa tlephrmain wkkrmacecs lwahscsskr asavhsshas
      181 rlpacsgkgp ttsdenwglp rwtrllrgfr tgtrgepps
//
LOCUS       XP_077505464             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115159 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505464
VERSION     XP_077505464.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649338.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115159 isoform X2"
                     /calculated_mol_wt=19968
     CDS             1..181
                     /gene="LOC144115159"
                     /coded_by="XM_077649338.1:414..959"
                     /db_xref="GeneID:144115159"
ORIGIN      
        1 matckrhker lhesismpql glaprlalvl fligkkprlq eavilaprad plvvvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 cslwahscss krasavhssh asrlpacsgk gpttsdenwg lprwtrllrg frtgtrgepp
      181 s
//
LOCUS       XP_077505465             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115160 [Amblyomma americanum].
ACCESSION   XP_077505465
VERSION     XP_077505465.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649339.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115160"
                     /calculated_mol_wt=19963
     CDS             1..181
                     /gene="LOC144115160"
                     /coded_by="XM_077649339.1:668..1213"
                     /db_xref="GeneID:144115160"
ORIGIN      
        1 matckrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvtkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg frtgtrgepp
      181 s
//
LOCUS       XP_077505466             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115160 [Amblyomma americanum].
ACCESSION   XP_077505466
VERSION     XP_077505466.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649340.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115160"
                     /calculated_mol_wt=19963
     CDS             1..181
                     /gene="LOC144115160"
                     /coded_by="XM_077649340.1:1242..1787"
                     /db_xref="GeneID:144115160"
ORIGIN      
        1 matckrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvtkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg frtgtrgepp
      181 s
//
LOCUS       XP_077505467             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115160 [Amblyomma americanum].
ACCESSION   XP_077505467
VERSION     XP_077505467.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649341.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115160"
                     /calculated_mol_wt=19963
     CDS             1..181
                     /gene="LOC144115160"
                     /coded_by="XM_077649341.1:903..1448"
                     /db_xref="GeneID:144115160"
ORIGIN      
        1 matckrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvtkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg frtgtrgepp
      181 s
//
LOCUS       XP_077505468             214 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115162 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505468
VERSION     XP_077505468.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649342.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..214
                     /product="uncharacterized protein LOC144115162 isoform X1"
                     /calculated_mol_wt=23327
     CDS             1..214
                     /gene="LOC144115162"
                     /coded_by="XM_077649342.1:91..735"
                     /db_xref="GeneID:144115162"
ORIGIN      
        1 mtlhgspacf ggllsrvsse rsrvsqvskv hskmatckrh kerlhgsisk pqlglaprla
       61 lvlflmgkkp rlqeavmlap radpvvvvpk yrlnlsglft pnwsdapdqg qgshgcwcay
      121 rplaatqapw srrsatleph rmainwkkgm acecslwahs csskrasavh sshasrlpac
      181 sgkgpttsde nwglprwtrl lrgfrtgtrg epps
//
LOCUS       XP_077505469             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115162 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505469
VERSION     XP_077505469.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649343.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115162 isoform X2"
                     /calculated_mol_wt=19816
     CDS             1..181
                     /gene="LOC144115162"
                     /coded_by="XM_077649343.1:378..923"
                     /db_xref="GeneID:144115162"
ORIGIN      
        1 matckrhker lhgsiskpql glaprlalvl flmgkkprlq eavmlaprad pvvvvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkgmace
      121 cslwahscss krasavhssh asrlpacsgk gpttsdenwg lprwtrllrg frtgtrgepp
      181 s
//
LOCUS       XP_077505470             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115162 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505470
VERSION     XP_077505470.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649344.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115162 isoform X2"
                     /calculated_mol_wt=19816
     CDS             1..181
                     /gene="LOC144115162"
                     /coded_by="XM_077649344.1:375..920"
                     /db_xref="GeneID:144115162"
ORIGIN      
        1 matckrhker lhgsiskpql glaprlalvl flmgkkprlq eavmlaprad pvvvvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkgmace
      121 cslwahscss krasavhssh asrlpacsgk gpttsdenwg lprwtrllrg frtgtrgepp
      181 s
//
LOCUS       XP_077505471             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115162 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505471
VERSION     XP_077505471.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649345.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115162 isoform X2"
                     /calculated_mol_wt=19816
     CDS             1..181
                     /gene="LOC144115162"
                     /coded_by="XM_077649345.1:1161..1706"
                     /db_xref="GeneID:144115162"
ORIGIN      
        1 matckrhker lhgsiskpql glaprlalvl flmgkkprlq eavmlaprad pvvvvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkgmace
      121 cslwahscss krasavhssh asrlpacsgk gpttsdenwg lprwtrllrg frtgtrgepp
      181 s
//
LOCUS       XP_077505472             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115162 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505472
VERSION     XP_077505472.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649346.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115162 isoform X2"
                     /calculated_mol_wt=19816
     CDS             1..181
                     /gene="LOC144115162"
                     /coded_by="XM_077649346.1:1917..2462"
                     /db_xref="GeneID:144115162"
ORIGIN      
        1 matckrhker lhgsiskpql glaprlalvl flmgkkprlq eavmlaprad pvvvvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkgmace
      121 cslwahscss krasavhssh asrlpacsgk gpttsdenwg lprwtrllrg frtgtrgepp
      181 s
//
LOCUS       XP_077505473             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115163 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505473
VERSION     XP_077505473.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649347.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115163 isoform X1"
                     /calculated_mol_wt=19912
     CDS             1..181
                     /gene="LOC144115163"
                     /coded_by="XM_077649347.1:1106..1651"
                     /db_xref="GeneID:144115163"
ORIGIN      
        1 maickrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg fptgtrgepp
      181 s
//
LOCUS       XP_077505474             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115163 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505474
VERSION     XP_077505474.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649348.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115163 isoform X1"
                     /calculated_mol_wt=19912
     CDS             1..181
                     /gene="LOC144115163"
                     /coded_by="XM_077649348.1:1275..1820"
                     /db_xref="GeneID:144115163"
ORIGIN      
        1 maickrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg fptgtrgepp
      181 s
//
LOCUS       XP_077505475             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115163 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505475
VERSION     XP_077505475.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649349.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115163 isoform X1"
                     /calculated_mol_wt=19912
     CDS             1..181
                     /gene="LOC144115163"
                     /coded_by="XM_077649349.1:1197..1742"
                     /db_xref="GeneID:144115163"
ORIGIN      
        1 maickrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg fptgtrgepp
      181 s
//
LOCUS       XP_077505476             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115163 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505476
VERSION     XP_077505476.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649350.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115163 isoform X1"
                     /calculated_mol_wt=19912
     CDS             1..181
                     /gene="LOC144115163"
                     /coded_by="XM_077649350.1:1184..1729"
                     /db_xref="GeneID:144115163"
ORIGIN      
        1 maickrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg fptgtrgepp
      181 s
//
LOCUS       XP_077505477             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115163 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505477
VERSION     XP_077505477.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649351.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115163 isoform X2"
                     /calculated_mol_wt=19964
     CDS             1..181
                     /gene="LOC144115163"
                     /coded_by="XM_077649351.1:1106..1651"
                     /db_xref="GeneID:144115163"
ORIGIN      
        1 maickrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwd qrgaaivaah fspqerwfse
      181 t
//
LOCUS       XP_077505478             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115163 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505478
VERSION     XP_077505478.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649352.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115163 isoform X1"
                     /calculated_mol_wt=19912
     CDS             1..181
                     /gene="LOC144115163"
                     /coded_by="XM_077649352.1:1234..1779"
                     /db_xref="GeneID:144115163"
ORIGIN      
        1 maickrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg fptgtrgepp
      181 s
//
LOCUS       XP_077505479             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115163 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505479
VERSION     XP_077505479.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649353.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115163 isoform X1"
                     /calculated_mol_wt=19912
     CDS             1..181
                     /gene="LOC144115163"
                     /coded_by="XM_077649353.1:1151..1696"
                     /db_xref="GeneID:144115163"
ORIGIN      
        1 maickrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg fptgtrgepp
      181 s
//
LOCUS       XP_077505480             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115163 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505480
VERSION     XP_077505480.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649354.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115163 isoform X1"
                     /calculated_mol_wt=19912
     CDS             1..181
                     /gene="LOC144115163"
                     /coded_by="XM_077649354.1:1189..1734"
                     /db_xref="GeneID:144115163"
ORIGIN      
        1 maickrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg fptgtrgepp
      181 s
//
LOCUS       XP_077505481             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115163 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505481
VERSION     XP_077505481.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649355.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115163 isoform X1"
                     /calculated_mol_wt=19912
     CDS             1..181
                     /gene="LOC144115163"
                     /coded_by="XM_077649355.1:1262..1807"
                     /db_xref="GeneID:144115163"
ORIGIN      
        1 maickrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg fptgtrgepp
      181 s
//
LOCUS       XP_077505482             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115163 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505482
VERSION     XP_077505482.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649356.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115163 isoform X1"
                     /calculated_mol_wt=19912
     CDS             1..181
                     /gene="LOC144115163"
                     /coded_by="XM_077649356.1:1217..1762"
                     /db_xref="GeneID:144115163"
ORIGIN      
        1 maickrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg fptgtrgepp
      181 s
//
LOCUS       XP_077505483             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115163 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505483
VERSION     XP_077505483.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649357.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115163 isoform X1"
                     /calculated_mol_wt=19912
     CDS             1..181
                     /gene="LOC144115163"
                     /coded_by="XM_077649357.1:1238..1783"
                     /db_xref="GeneID:144115163"
ORIGIN      
        1 maickrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg fptgtrgepp
      181 s
//
LOCUS       XP_077505484             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115163 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505484
VERSION     XP_077505484.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649358.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115163 isoform X1"
                     /calculated_mol_wt=19912
     CDS             1..181
                     /gene="LOC144115163"
                     /coded_by="XM_077649358.1:1193..1738"
                     /db_xref="GeneID:144115163"
ORIGIN      
        1 maickrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg fptgtrgepp
      181 s
//
LOCUS       XP_077505485             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115163 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505485
VERSION     XP_077505485.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649359.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115163 isoform X1"
                     /calculated_mol_wt=19912
     CDS             1..181
                     /gene="LOC144115163"
                     /coded_by="XM_077649359.1:667..1212"
                     /db_xref="GeneID:144115163"
ORIGIN      
        1 maickrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg fptgtrgepp
      181 s
//
LOCUS       XP_077505486             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115163 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505486
VERSION     XP_077505486.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649360.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115163 isoform X1"
                     /calculated_mol_wt=19912
     CDS             1..181
                     /gene="LOC144115163"
                     /coded_by="XM_077649360.1:641..1186"
                     /db_xref="GeneID:144115163"
ORIGIN      
        1 maickrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg fptgtrgepp
      181 s
//
LOCUS       XP_077505487             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115163 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505487
VERSION     XP_077505487.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649361.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115163 isoform X1"
                     /calculated_mol_wt=19912
     CDS             1..181
                     /gene="LOC144115163"
                     /coded_by="XM_077649361.1:614..1159"
                     /db_xref="GeneID:144115163"
ORIGIN      
        1 maickrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg fptgtrgepp
      181 s
//
LOCUS       XP_077505488             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115163 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505488
VERSION     XP_077505488.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649362.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115163 isoform X1"
                     /calculated_mol_wt=19912
     CDS             1..181
                     /gene="LOC144115163"
                     /coded_by="XM_077649362.1:633..1178"
                     /db_xref="GeneID:144115163"
ORIGIN      
        1 maickrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg fptgtrgepp
      181 s
//
LOCUS       XP_077505489             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115163 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505489
VERSION     XP_077505489.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649363.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115163 isoform X1"
                     /calculated_mol_wt=19912
     CDS             1..181
                     /gene="LOC144115163"
                     /coded_by="XM_077649363.1:1038..1583"
                     /db_xref="GeneID:144115163"
ORIGIN      
        1 maickrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg fptgtrgepp
      181 s
//
LOCUS       XP_077505490             145 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115165 [Amblyomma americanum].
ACCESSION   XP_077505490
VERSION     XP_077505490.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649364.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..145
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..145
                     /product="uncharacterized protein LOC144115165"
                     /calculated_mol_wt=15779
     CDS             1..145
                     /gene="LOC144115165"
                     /coded_by="XM_077649364.1:482..919"
                     /db_xref="GeneID:144115165"
ORIGIN      
        1 mkntsaavmw rrgasrhysp askahrrrts alglnhcnrf esihhcaaar vihvsaqvvp
       61 papppahawp gltspfsqkr lrwltgvvil eqsrqpqhla avnpcffhag itevsyspan
      121 ilthisapiy lvayaavnct ifsqa
//
LOCUS       XP_077505491             145 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115165 [Amblyomma americanum].
ACCESSION   XP_077505491
VERSION     XP_077505491.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649365.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..145
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..145
                     /product="uncharacterized protein LOC144115165"
                     /calculated_mol_wt=15779
     CDS             1..145
                     /gene="LOC144115165"
                     /coded_by="XM_077649365.1:482..919"
                     /db_xref="GeneID:144115165"
ORIGIN      
        1 mkntsaavmw rrgasrhysp askahrrrts alglnhcnrf esihhcaaar vihvsaqvvp
       61 papppahawp gltspfsqkr lrwltgvvil eqsrqpqhla avnpcffhag itevsyspan
      121 ilthisapiy lvayaavnct ifsqa
//
LOCUS       XP_077505492             198 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115167 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505492
VERSION     XP_077505492.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649366.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..198
                     /product="uncharacterized protein LOC144115167 isoform X1"
                     /calculated_mol_wt=21377
     CDS             1..198
                     /gene="LOC144115167"
                     /coded_by="XM_077649366.1:259..855"
                     /db_xref="GeneID:144115167"
ORIGIN      
        1 mrrvlvsttr ststiaaakq ekkavtgirr vfvssiinrt maatcswfsg hgkskktgas
       61 efkiekamyd tvkeipmdav fhkrtaipiw steavvlcss sgkcsggdsg rqgkgivatq
      121 lesscyqgns tystlpekmh ammlgasilt ilpilstnea kdafvqiaes qqppwkatte
      181 wlrscwgret gmvvgkfg
//
LOCUS       XP_077505493             186 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115167 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505493
VERSION     XP_077505493.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649367.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..186
                     /product="uncharacterized protein LOC144115167 isoform X2"
                     /calculated_mol_wt=19964
     CDS             1..186
                     /gene="LOC144115167"
                     /coded_by="XM_077649367.1:419..979"
                     /db_xref="GeneID:144115167"
ORIGIN      
        1 msplsrfgav lvcacsafia ehniinrtma atcswfsghg kskktgasef kiekamydtv
       61 keipmdavfh krtaipiwst eavvlcsssg kcsggdsgrq gkgivatqle sscyqgnsty
      121 stlpekmham mlgasiltil pilstneakd afvqiaesqq ppwkattewl rscwgretgm
      181 vvgkfg
//
LOCUS       XP_077505494             154 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115167 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505494
VERSION     XP_077505494.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649368.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..154
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..154
                     /product="uncharacterized protein LOC144115167 isoform X3"
                     /calculated_mol_wt=16842
     CDS             1..154
                     /gene="LOC144115167"
                     /coded_by="XM_077649368.1:259..723"
                     /db_xref="GeneID:144115167"
ORIGIN      
        1 mrrvlvsttr ststiaaakq ekkavtgirr vfvssiinrt maatcswfsg hgkskktgas
       61 efkiekamyd tvkeipmdav fhkrtaipiw steavvlcss sgkcsggdsg rhcgytagvk
      121 llsgkfnlfd farkdaryda wglhidytan fvyq
//
LOCUS       XP_077505496             214 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115168 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505496
VERSION     XP_077505496.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649370.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..214
                     /product="uncharacterized protein LOC144115168 isoform X1"
                     /calculated_mol_wt=23327
     CDS             1..214
                     /gene="LOC144115168"
                     /coded_by="XM_077649370.1:116..760"
                     /db_xref="GeneID:144115168"
ORIGIN      
        1 mtlhgspacf ggllsrvsse rsrvsqvskv hskmatckrh kerlhgsisk pqlglaprla
       61 lvlflmgkkp rlqeavmlap radpvvvvpk yrlnlsglft pnwsdapdqg qgshgcwcay
      121 rplaatqapw srrsatleph rmainwkkgm acecslwahs csskrasavh sshasrlpac
      181 sgkgpttsde nwglprwtrl lrgfrtgtrg epps
//
LOCUS       XP_077505497             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115168 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505497
VERSION     XP_077505497.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649371.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115168 isoform X2"
                     /calculated_mol_wt=19816
     CDS             1..181
                     /gene="LOC144115168"
                     /coded_by="XM_077649371.1:1118..1663"
                     /db_xref="GeneID:144115168"
ORIGIN      
        1 matckrhker lhgsiskpql glaprlalvl flmgkkprlq eavmlaprad pvvvvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkgmace
      121 cslwahscss krasavhssh asrlpacsgk gpttsdenwg lprwtrllrg frtgtrgepp
      181 s
//
LOCUS       XP_077505498             196 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115169 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505498
VERSION     XP_077505498.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649372.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..196
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..196
                     /product="uncharacterized protein LOC144115169 isoform X1"
                     /calculated_mol_wt=21522
     CDS             1..196
                     /gene="LOC144115169"
                     /coded_by="XM_077649372.1:46..636"
                     /db_xref="GeneID:144115169"
ORIGIN      
        1 mprrlmtlhg spacggglls rgssersrvs qvskvhskma tckrhkerlh esismpqlgl
       61 aprlalvlfl igkkprlqea vmlapradpl vvvpkyrlnl sglftpnwsd apalldqgqg
      121 nhgcwcayrp laatqapwsr rsatlephrm ainwkkgmsc efclwahscs skrasavhss
      181 hvspsaclqw egaynf
//
LOCUS       XP_077505499             160 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115169 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505499
VERSION     XP_077505499.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649373.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..160
                     /product="uncharacterized protein LOC144115169 isoform X2"
                     /calculated_mol_wt=17457
     CDS             1..160
                     /gene="LOC144115169"
                     /coded_by="XM_077649373.1:71..553"
                     /db_xref="GeneID:144115169"
ORIGIN      
        1 mprrlmtlhg spacggglls rgssersrvs qvskvhskma tckrhkerlh esismpqlgl
       61 aprlalvlfl igkkprlqea vmlapradpl vvvpkyrlnl sglftpnwsd apalldqgqg
      121 nhgcwcayrp laatqapwsr rsatlephrm aincllhsap
//
LOCUS       XP_077505500             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115169 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505500
VERSION     XP_077505500.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649374.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144115169 isoform X3"
                     /calculated_mol_wt=17489
     CDS             1..158
                     /gene="LOC144115169"
                     /coded_by="XM_077649374.1:419..895"
                     /db_xref="GeneID:144115169"
ORIGIN      
        1 matckrhker lhesismpql glaprlalvl fligkkprlq eavmlaprad plvvvpkyrl
       61 nlsglftpnw sdapalldqg qgnhgcwcay rplaatqapw srrsatleph rmainwkkgm
      121 scefclwahs csskrasavh sshvspsacl qwegaynf
//
LOCUS       XP_077505501             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115169 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505501
VERSION     XP_077505501.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649375.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144115169 isoform X3"
                     /calculated_mol_wt=17489
     CDS             1..158
                     /gene="LOC144115169"
                     /coded_by="XM_077649375.1:271..747"
                     /db_xref="GeneID:144115169"
ORIGIN      
        1 matckrhker lhesismpql glaprlalvl fligkkprlq eavmlaprad plvvvpkyrl
       61 nlsglftpnw sdapalldqg qgnhgcwcay rplaatqapw srrsatleph rmainwkkgm
      121 scefclwahs csskrasavh sshvspsacl qwegaynf
//
LOCUS       XP_077505502             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115169 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505502
VERSION     XP_077505502.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649376.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144115169 isoform X3"
                     /calculated_mol_wt=17489
     CDS             1..158
                     /gene="LOC144115169"
                     /coded_by="XM_077649376.1:923..1399"
                     /db_xref="GeneID:144115169"
ORIGIN      
        1 matckrhker lhesismpql glaprlalvl fligkkprlq eavmlaprad plvvvpkyrl
       61 nlsglftpnw sdapalldqg qgnhgcwcay rplaatqapw srrsatleph rmainwkkgm
      121 scefclwahs csskrasavh sshvspsacl qwegaynf
//
LOCUS       XP_077505503             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115169 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505503
VERSION     XP_077505503.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649377.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144115169 isoform X3"
                     /calculated_mol_wt=17489
     CDS             1..158
                     /gene="LOC144115169"
                     /coded_by="XM_077649377.1:1119..1595"
                     /db_xref="GeneID:144115169"
ORIGIN      
        1 matckrhker lhesismpql glaprlalvl fligkkprlq eavmlaprad plvvvpkyrl
       61 nlsglftpnw sdapalldqg qgnhgcwcay rplaatqapw srrsatleph rmainwkkgm
      121 scefclwahs csskrasavh sshvspsacl qwegaynf
//
LOCUS       XP_077505504             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115169 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505504
VERSION     XP_077505504.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649378.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144115169 isoform X3"
                     /calculated_mol_wt=17489
     CDS             1..158
                     /gene="LOC144115169"
                     /coded_by="XM_077649378.1:4688..5164"
                     /db_xref="GeneID:144115169"
ORIGIN      
        1 matckrhker lhesismpql glaprlalvl fligkkprlq eavmlaprad plvvvpkyrl
       61 nlsglftpnw sdapalldqg qgnhgcwcay rplaatqapw srrsatleph rmainwkkgm
      121 scefclwahs csskrasavh sshvspsacl qwegaynf
//
LOCUS       XP_077505505             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115172 [Amblyomma americanum].
ACCESSION   XP_077505505
VERSION     XP_077505505.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649379.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115172"
                     /calculated_mol_wt=19971
     CDS             1..181
                     /gene="LOC144115172"
                     /coded_by="XM_077649379.1:1106..1651"
                     /db_xref="GeneID:144115172"
ORIGIN      
        1 maickrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg frtgtrgepp
      181 s
//
LOCUS       XP_077505506             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115172 [Amblyomma americanum].
ACCESSION   XP_077505506
VERSION     XP_077505506.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649380.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115172"
                     /calculated_mol_wt=19971
     CDS             1..181
                     /gene="LOC144115172"
                     /coded_by="XM_077649380.1:1275..1820"
                     /db_xref="GeneID:144115172"
ORIGIN      
        1 maickrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg frtgtrgepp
      181 s
//
LOCUS       XP_077505507             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115172 [Amblyomma americanum].
ACCESSION   XP_077505507
VERSION     XP_077505507.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649381.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115172"
                     /calculated_mol_wt=19971
     CDS             1..181
                     /gene="LOC144115172"
                     /coded_by="XM_077649381.1:1197..1742"
                     /db_xref="GeneID:144115172"
ORIGIN      
        1 maickrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg frtgtrgepp
      181 s
//
LOCUS       XP_077505508             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115172 [Amblyomma americanum].
ACCESSION   XP_077505508
VERSION     XP_077505508.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649382.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115172"
                     /calculated_mol_wt=19971
     CDS             1..181
                     /gene="LOC144115172"
                     /coded_by="XM_077649382.1:1184..1729"
                     /db_xref="GeneID:144115172"
ORIGIN      
        1 maickrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg frtgtrgepp
      181 s
//
LOCUS       XP_077505509             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115172 [Amblyomma americanum].
ACCESSION   XP_077505509
VERSION     XP_077505509.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649383.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115172"
                     /calculated_mol_wt=19971
     CDS             1..181
                     /gene="LOC144115172"
                     /coded_by="XM_077649383.1:1151..1696"
                     /db_xref="GeneID:144115172"
ORIGIN      
        1 maickrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg frtgtrgepp
      181 s
//
LOCUS       XP_077505510             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115172 [Amblyomma americanum].
ACCESSION   XP_077505510
VERSION     XP_077505510.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649384.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115172"
                     /calculated_mol_wt=19971
     CDS             1..181
                     /gene="LOC144115172"
                     /coded_by="XM_077649384.1:1189..1734"
                     /db_xref="GeneID:144115172"
ORIGIN      
        1 maickrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg frtgtrgepp
      181 s
//
LOCUS       XP_077505511             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115172 [Amblyomma americanum].
ACCESSION   XP_077505511
VERSION     XP_077505511.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649385.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115172"
                     /calculated_mol_wt=19971
     CDS             1..181
                     /gene="LOC144115172"
                     /coded_by="XM_077649385.1:1217..1762"
                     /db_xref="GeneID:144115172"
ORIGIN      
        1 maickrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg frtgtrgepp
      181 s
//
LOCUS       XP_077505512             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115172 [Amblyomma americanum].
ACCESSION   XP_077505512
VERSION     XP_077505512.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649386.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115172"
                     /calculated_mol_wt=19971
     CDS             1..181
                     /gene="LOC144115172"
                     /coded_by="XM_077649386.1:1193..1738"
                     /db_xref="GeneID:144115172"
ORIGIN      
        1 maickrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg frtgtrgepp
      181 s
//
LOCUS       XP_077505513             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115172 [Amblyomma americanum].
ACCESSION   XP_077505513
VERSION     XP_077505513.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649387.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115172"
                     /calculated_mol_wt=19971
     CDS             1..181
                     /gene="LOC144115172"
                     /coded_by="XM_077649387.1:643..1188"
                     /db_xref="GeneID:144115172"
ORIGIN      
        1 maickrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg frtgtrgepp
      181 s
//
LOCUS       XP_077505514             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115172 [Amblyomma americanum].
ACCESSION   XP_077505514
VERSION     XP_077505514.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649388.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115172"
                     /calculated_mol_wt=19971
     CDS             1..181
                     /gene="LOC144115172"
                     /coded_by="XM_077649388.1:639..1184"
                     /db_xref="GeneID:144115172"
ORIGIN      
        1 maickrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg frtgtrgepp
      181 s
//
LOCUS       XP_077505515             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115172 [Amblyomma americanum].
ACCESSION   XP_077505515
VERSION     XP_077505515.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649389.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115172"
                     /calculated_mol_wt=19971
     CDS             1..181
                     /gene="LOC144115172"
                     /coded_by="XM_077649389.1:614..1159"
                     /db_xref="GeneID:144115172"
ORIGIN      
        1 maickrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg frtgtrgepp
      181 s
//
LOCUS       XP_077505516             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115172 [Amblyomma americanum].
ACCESSION   XP_077505516
VERSION     XP_077505516.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649390.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115172"
                     /calculated_mol_wt=19971
     CDS             1..181
                     /gene="LOC144115172"
                     /coded_by="XM_077649390.1:1038..1583"
                     /db_xref="GeneID:144115172"
ORIGIN      
        1 maickrhker lhgsistpql glaprlalll fligkkprlq eavmlaprad pvvmvpkyrl
       61 nlsglftpnw sdapdqgqgs hgcwcayrpl aatqapwsrr satlephrma inwkkrmace
      121 ccpwahscss krasavhssh vsrqpacsgk gpttsdenwg lprwtrllrg frtgtrgepp
      181 s
//
LOCUS       XP_077505517             145 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115174 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505517
VERSION     XP_077505517.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649391.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..145
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..145
                     /product="uncharacterized protein LOC144115174 isoform X1"
                     /calculated_mol_wt=15779
     CDS             1..145
                     /gene="LOC144115174"
                     /coded_by="XM_077649391.1:607..1044"
                     /db_xref="GeneID:144115174"
ORIGIN      
        1 mkntsaavmw rrgasrhysp askahrrrts alglnhcnrf esihhcaaar vihvsaqvvp
       61 papppahawp gltspfsqkr lrwltgvvil eqsrqpqhla avnpcffhag itevsyspan
      121 ilthisapiy lvayaavnct ifsqa
//
LOCUS       XP_077505519              76 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115174 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505519
VERSION     XP_077505519.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649393.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..76
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..76
                     /product="uncharacterized protein LOC144115174 isoform X2"
                     /calculated_mol_wt=7871
     CDS             1..76
                     /gene="LOC144115174"
                     /coded_by="XM_077649393.1:1184..1414"
                     /db_xref="GeneID:144115174"
ORIGIN      
        1 mrkfiptksr gitndaatcq lhrpggpsna nvnanvasts aligcssdts tshfeghlsa
       61 datawthatl pqhrtq
//
LOCUS       XP_077505520             184 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115175 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505520
VERSION     XP_077505520.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649394.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..184
                     /product="uncharacterized protein LOC144115175 isoform X1"
                     /calculated_mol_wt=20485
     CDS             1..184
                     /gene="LOC144115175"
                     /coded_by="XM_077649394.1:1235..1789"
                     /db_xref="GeneID:144115175"
ORIGIN      
        1 matckrhker lhesismpql glaprlalvl fligkkprlq eavmlaprad plvvvpkyrl
       61 nlsglftpnw sdapalldqg qgnhgcwcay rplaatqapw srrsatleph rmainwkkrm
      121 scefclwahs csskrasavh sshvsrlpvc sgkgpttsde nwvlprwtrl lrgfrtgtrg
      181 epps
//
LOCUS       XP_077505521             184 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115175 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505521
VERSION     XP_077505521.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649395.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..184
                     /product="uncharacterized protein LOC144115175 isoform X1"
                     /calculated_mol_wt=20485
     CDS             1..184
                     /gene="LOC144115175"
                     /coded_by="XM_077649395.1:1431..1985"
                     /db_xref="GeneID:144115175"
ORIGIN      
        1 matckrhker lhesismpql glaprlalvl fligkkprlq eavmlaprad plvvvpkyrl
       61 nlsglftpnw sdapalldqg qgnhgcwcay rplaatqapw srrsatleph rmainwkkrm
      121 scefclwahs csskrasavh sshvsrlpvc sgkgpttsde nwvlprwtrl lrgfrtgtrg
      181 epps
//
LOCUS       XP_077505522             184 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115175 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505522
VERSION     XP_077505522.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649396.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..184
                     /product="uncharacterized protein LOC144115175 isoform X1"
                     /calculated_mol_wt=20485
     CDS             1..184
                     /gene="LOC144115175"
                     /coded_by="XM_077649396.1:1318..1872"
                     /db_xref="GeneID:144115175"
ORIGIN      
        1 matckrhker lhesismpql glaprlalvl fligkkprlq eavmlaprad plvvvpkyrl
       61 nlsglftpnw sdapalldqg qgnhgcwcay rplaatqapw srrsatleph rmainwkkrm
      121 scefclwahs csskrasavh sshvsrlpvc sgkgpttsde nwvlprwtrl lrgfrtgtrg
      181 epps
//
LOCUS       XP_077505523             184 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115175 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505523
VERSION     XP_077505523.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649397.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..184
                     /product="uncharacterized protein LOC144115175 isoform X1"
                     /calculated_mol_wt=20485
     CDS             1..184
                     /gene="LOC144115175"
                     /coded_by="XM_077649397.1:230..784"
                     /db_xref="GeneID:144115175"
ORIGIN      
        1 matckrhker lhesismpql glaprlalvl fligkkprlq eavmlaprad plvvvpkyrl
       61 nlsglftpnw sdapalldqg qgnhgcwcay rplaatqapw srrsatleph rmainwkkrm
      121 scefclwahs csskrasavh sshvsrlpvc sgkgpttsde nwvlprwtrl lrgfrtgtrg
      181 epps
//
LOCUS       XP_077505524             184 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115175 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505524
VERSION     XP_077505524.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649398.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..184
                     /product="uncharacterized protein LOC144115175 isoform X1"
                     /calculated_mol_wt=20485
     CDS             1..184
                     /gene="LOC144115175"
                     /coded_by="XM_077649398.1:231..785"
                     /db_xref="GeneID:144115175"
ORIGIN      
        1 matckrhker lhesismpql glaprlalvl fligkkprlq eavmlaprad plvvvpkyrl
       61 nlsglftpnw sdapalldqg qgnhgcwcay rplaatqapw srrsatleph rmainwkkrm
      121 scefclwahs csskrasavh sshvsrlpvc sgkgpttsde nwvlprwtrl lrgfrtgtrg
      181 epps
//
LOCUS       XP_077505525             184 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115175 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505525
VERSION     XP_077505525.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649399.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..184
                     /product="uncharacterized protein LOC144115175 isoform X1"
                     /calculated_mol_wt=20485
     CDS             1..184
                     /gene="LOC144115175"
                     /coded_by="XM_077649399.1:349..903"
                     /db_xref="GeneID:144115175"
ORIGIN      
        1 matckrhker lhesismpql glaprlalvl fligkkprlq eavmlaprad plvvvpkyrl
       61 nlsglftpnw sdapalldqg qgnhgcwcay rplaatqapw srrsatleph rmainwkkrm
      121 scefclwahs csskrasavh sshvsrlpvc sgkgpttsde nwvlprwtrl lrgfrtgtrg
      181 epps
//
LOCUS       XP_077505526             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115175 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505526
VERSION     XP_077505526.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649400.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115175 isoform X2"
                     /calculated_mol_wt=20188
     CDS             1..181
                     /gene="LOC144115175"
                     /coded_by="XM_077649400.1:2419..2964"
                     /db_xref="GeneID:144115175"
ORIGIN      
        1 matckrhker lhesismpql glaprlalvl fligkkprlq eavmlaprad plvvvpkyrl
       61 nlsglftpnw sdapdqgqgn hgcwcayrpl aatqapwsrr satlephrma inwkkrmsce
      121 fclwahscss krasavhssh vsrlpvcsgk gpttsdenwv lprwtrllrg frtgtrgepp
      181 s
//
LOCUS       XP_077505527             277 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115176 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505527
VERSION     XP_077505527.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649401.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..277
                     /product="uncharacterized protein LOC144115176 isoform X1"
                     /calculated_mol_wt=30071
     CDS             1..277
                     /gene="LOC144115176"
                     /coded_by="XM_077649401.1:45..878"
                     /db_xref="GeneID:144115176"
ORIGIN      
        1 mprrlmtlhg spacggglls rgssersrvs qvskvhskma tckrhkerlh esismpqlgl
       61 aprlalvlfl igkkprlqea psrpvgrgaq vstqpvrlih pqlerrasfa rpgtrqpwll
      121 vcvssfgghp sslepqecdp gatshghqle krnvlrvlsv gtllqleaci cgafeprftv
      181 clpavgrglq lltktdlcsg plisrpavtl erfgtpmrqn klrawtrnkp gsshaghgcy
      241 maslqgpegs rhhsctlrpa aamvfrdlga mslnfrp
//
LOCUS       XP_077505528             242 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115176 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505528
VERSION     XP_077505528.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649402.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..242
                     /product="uncharacterized protein LOC144115176 isoform X2"
                     /calculated_mol_wt=26078
     CDS             1..242
                     /gene="LOC144115176"
                     /coded_by="XM_077649402.1:38..766"
                     /db_xref="GeneID:144115176"
ORIGIN      
        1 mprrlmtlhg spacggglls rgssersrvs qvskvhskma tckrhkerlh esismpqlgl
       61 aprlalvlfl igkkprlqea psrpvgrgaq vstqpvrlih pqlerrasfa rpgtrqpwll
      121 vcvssfgghp sslepqecdp gatshghqle krnvlrvlsv gtllqleaci cgafeprftv
      181 clpavgrglq lltktgssha ghgcymaslq gpegsrhhsc tlrpaaamvf rdlgamslnf
      241 rp
//
LOCUS       XP_077505529             239 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115176 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505529
VERSION     XP_077505529.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649403.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..239
                     /product="uncharacterized protein LOC144115176 isoform X3"
                     /calculated_mol_wt=26038
     CDS             1..239
                     /gene="LOC144115176"
                     /coded_by="XM_077649403.1:452..1171"
                     /db_xref="GeneID:144115176"
ORIGIN      
        1 matckrhker lhesismpql glaprlalvl fligkkprlq eapsrpvgrg aqvstqpvrl
       61 ihpqlerras farpgtrqpw llvcvssfgg hpsslepqec dpgatshghq lekrnvlrvl
      121 svgtllqlea cicgafeprf tvclpavgrg lqlltktdlc sgplisrpav tlerfgtpmr
      181 qnklrawtrn kpgsshaghg cymaslqgpe gsrhhsctlr paaamvfrdl gamslnfrp
//
LOCUS       XP_077505530             239 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115176 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505530
VERSION     XP_077505530.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649404.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..239
                     /product="uncharacterized protein LOC144115176 isoform X3"
                     /calculated_mol_wt=26038
     CDS             1..239
                     /gene="LOC144115176"
                     /coded_by="XM_077649404.1:416..1135"
                     /db_xref="GeneID:144115176"
ORIGIN      
        1 matckrhker lhesismpql glaprlalvl fligkkprlq eapsrpvgrg aqvstqpvrl
       61 ihpqlerras farpgtrqpw llvcvssfgg hpsslepqec dpgatshghq lekrnvlrvl
      121 svgtllqlea cicgafeprf tvclpavgrg lqlltktdlc sgplisrpav tlerfgtpmr
      181 qnklrawtrn kpgsshaghg cymaslqgpe gsrhhsctlr paaamvfrdl gamslnfrp
//
LOCUS       XP_077505531             239 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115176 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505531
VERSION     XP_077505531.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649405.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..239
                     /product="uncharacterized protein LOC144115176 isoform X3"
                     /calculated_mol_wt=26038
     CDS             1..239
                     /gene="LOC144115176"
                     /coded_by="XM_077649405.1:391..1110"
                     /db_xref="GeneID:144115176"
ORIGIN      
        1 matckrhker lhesismpql glaprlalvl fligkkprlq eapsrpvgrg aqvstqpvrl
       61 ihpqlerras farpgtrqpw llvcvssfgg hpsslepqec dpgatshghq lekrnvlrvl
      121 svgtllqlea cicgafeprf tvclpavgrg lqlltktdlc sgplisrpav tlerfgtpmr
      181 qnklrawtrn kpgsshaghg cymaslqgpe gsrhhsctlr paaamvfrdl gamslnfrp
//
LOCUS       XP_077505533             239 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115176 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505533
VERSION     XP_077505533.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649407.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..239
                     /product="uncharacterized protein LOC144115176 isoform X3"
                     /calculated_mol_wt=26038
     CDS             1..239
                     /gene="LOC144115176"
                     /coded_by="XM_077649407.1:347..1066"
                     /db_xref="GeneID:144115176"
ORIGIN      
        1 matckrhker lhesismpql glaprlalvl fligkkprlq eapsrpvgrg aqvstqpvrl
       61 ihpqlerras farpgtrqpw llvcvssfgg hpsslepqec dpgatshghq lekrnvlrvl
      121 svgtllqlea cicgafeprf tvclpavgrg lqlltktdlc sgplisrpav tlerfgtpmr
      181 qnklrawtrn kpgsshaghg cymaslqgpe gsrhhsctlr paaamvfrdl gamslnfrp
//
LOCUS       XP_077505534             239 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115176 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505534
VERSION     XP_077505534.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649408.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..239
                     /product="uncharacterized protein LOC144115176 isoform X3"
                     /calculated_mol_wt=26038
     CDS             1..239
                     /gene="LOC144115176"
                     /coded_by="XM_077649408.1:1126..1845"
                     /db_xref="GeneID:144115176"
ORIGIN      
        1 matckrhker lhesismpql glaprlalvl fligkkprlq eapsrpvgrg aqvstqpvrl
       61 ihpqlerras farpgtrqpw llvcvssfgg hpsslepqec dpgatshghq lekrnvlrvl
      121 svgtllqlea cicgafeprf tvclpavgrg lqlltktdlc sgplisrpav tlerfgtpmr
      181 qnklrawtrn kpgsshaghg cymaslqgpe gsrhhsctlr paaamvfrdl gamslnfrp
//
LOCUS       XP_077505535             152 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115176 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505535
VERSION     XP_077505535.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649409.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..152
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..152
                     /product="uncharacterized protein LOC144115176 isoform X4"
                     /calculated_mol_wt=16434
     CDS             1..152
                     /gene="LOC144115176"
                     /coded_by="XM_077649409.1:52..510"
                     /db_xref="GeneID:144115176"
ORIGIN      
        1 mprrlmtlhg spacggglls rgssersrvs qvskvhskma tckrhkerlh esismpqlgl
       61 aprlalvlfl igkkprlqea psrpvgrgaq vstqpvrlih pqlerrasfa rpgtrqpwll
      121 vcvssfgghp sslepqecdp gatshghqlf as
//
LOCUS       XP_077505536             196 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115178 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505536
VERSION     XP_077505536.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649410.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..196
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..196
                     /product="uncharacterized protein LOC144115178 isoform X1"
                     /calculated_mol_wt=21533
     CDS             1..196
                     /gene="LOC144115178"
                     /coded_by="XM_077649410.1:941..1531"
                     /db_xref="GeneID:144115178"
ORIGIN      
        1 mprrlmtlhg spacggglls rgssersrvs qvskvhskma tckrhkerlh esismpqlgl
       61 aprlalelfl igkkprlpev vmlapradpl gvvpkyrlnl sglftpnwsd apalldqgqg
      121 nhgcwcayrp laatqapwsr rsatlephrm ainwkkgmsc efclwahscs lkrasavhss
      181 hvspsaclqw egaynf
//
LOCUS       XP_077505537             176 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115178 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505537
VERSION     XP_077505537.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649411.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..176
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..176
                     /product="uncharacterized protein LOC144115178 isoform X2"
                     /calculated_mol_wt=19363
     CDS             1..176
                     /gene="LOC144115178"
                     /coded_by="XM_077649411.1:944..1474"
                     /db_xref="GeneID:144115178"
ORIGIN      
        1 mprrlmtlhg spacggglls rgssersrvs qvskvhskma tckrhkerlh esismpqlgl
       61 aprlalelfl igkkprlpev vmlapradpl gvvpkyrlnl sglftpnwsd apalldqgqg
      121 nhgcwcayrp laatqapwsr rsatlephrm ainwvlprwt rllhgfptgt rgepps
//
LOCUS       XP_077505538             160 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115178 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505538
VERSION     XP_077505538.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649412.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..160
                     /product="uncharacterized protein LOC144115178 isoform X3"
                     /calculated_mol_wt=17441
     CDS             1..160
                     /gene="LOC144115178"
                     /coded_by="XM_077649412.1:949..1431"
                     /db_xref="GeneID:144115178"
ORIGIN      
        1 mprrlmtlhg spacggglls rgssersrvs qvskvhskma tckrhkerlh esismpqlgl
       61 aprlalelfl igkkprlpev vmlapradpl gvvpkyrlnl sglftpnwsd apalldqgqg
      121 nhgcwcayrp laatqapwsr rsatlephrm aincllhsap
//
LOCUS       XP_077505539             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115178 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505539
VERSION     XP_077505539.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649413.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144115178 isoform X4"
                     /calculated_mol_wt=17500
     CDS             1..158
                     /gene="LOC144115178"
                     /coded_by="XM_077649413.1:506..982"
                     /db_xref="GeneID:144115178"
ORIGIN      
        1 matckrhker lhesismpql glaprlalel fligkkprlp evvmlaprad plgvvpkyrl
       61 nlsglftpnw sdapalldqg qgnhgcwcay rplaatqapw srrsatleph rmainwkkgm
      121 scefclwahs cslkrasavh sshvspsacl qwegaynf
//
LOCUS       XP_077505540             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115178 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505540
VERSION     XP_077505540.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649414.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144115178 isoform X4"
                     /calculated_mol_wt=17500
     CDS             1..158
                     /gene="LOC144115178"
                     /coded_by="XM_077649414.1:394..870"
                     /db_xref="GeneID:144115178"
ORIGIN      
        1 matckrhker lhesismpql glaprlalel fligkkprlp evvmlaprad plgvvpkyrl
       61 nlsglftpnw sdapalldqg qgnhgcwcay rplaatqapw srrsatleph rmainwkkgm
      121 scefclwahs cslkrasavh sshvspsacl qwegaynf
//
LOCUS       XP_077505541             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115178 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505541
VERSION     XP_077505541.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649415.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144115178 isoform X4"
                     /calculated_mol_wt=17500
     CDS             1..158
                     /gene="LOC144115178"
                     /coded_by="XM_077649415.1:419..895"
                     /db_xref="GeneID:144115178"
ORIGIN      
        1 matckrhker lhesismpql glaprlalel fligkkprlp evvmlaprad plgvvpkyrl
       61 nlsglftpnw sdapalldqg qgnhgcwcay rplaatqapw srrsatleph rmainwkkgm
      121 scefclwahs cslkrasavh sshvspsacl qwegaynf
//
LOCUS       XP_077505542             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115178 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505542
VERSION     XP_077505542.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649416.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144115178 isoform X4"
                     /calculated_mol_wt=17500
     CDS             1..158
                     /gene="LOC144115178"
                     /coded_by="XM_077649416.1:349..825"
                     /db_xref="GeneID:144115178"
ORIGIN      
        1 matckrhker lhesismpql glaprlalel fligkkprlp evvmlaprad plgvvpkyrl
       61 nlsglftpnw sdapalldqg qgnhgcwcay rplaatqapw srrsatleph rmainwkkgm
      121 scefclwahs cslkrasavh sshvspsacl qwegaynf
//
LOCUS       XP_077505543             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115178 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505543
VERSION     XP_077505543.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649417.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144115178 isoform X4"
                     /calculated_mol_wt=17500
     CDS             1..158
                     /gene="LOC144115178"
                     /coded_by="XM_077649417.1:506..982"
                     /db_xref="GeneID:144115178"
ORIGIN      
        1 matckrhker lhesismpql glaprlalel fligkkprlp evvmlaprad plgvvpkyrl
       61 nlsglftpnw sdapalldqg qgnhgcwcay rplaatqapw srrsatleph rmainwkkgm
      121 scefclwahs cslkrasavh sshvspsacl qwegaynf
//
LOCUS       XP_077505544             184 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115180 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505544
VERSION     XP_077505544.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649418.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..184
                     /product="uncharacterized protein LOC144115180 isoform X1"
                     /calculated_mol_wt=20529
     CDS             1..184
                     /gene="LOC144115180"
                     /coded_by="XM_077649418.1:5800..6354"
                     /db_xref="GeneID:144115180"
ORIGIN      
        1 matckwhker lhesismpql glaprlalvl fligkkprlq eavmlaprad plvvvpkyrl
       61 nlsglftpnw sdapalldqg qgnhgcwcay rplaatqapw srrsvtleph rmainwkkrm
      121 scelclwahs cslkrasavh sshfsrlaac sgkgpttsde nwvlprwtrl lrgfrtgtrg
      181 epps
//
LOCUS       XP_077505545             184 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115180 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505545
VERSION     XP_077505545.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649419.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..184
                     /product="uncharacterized protein LOC144115180 isoform X1"
                     /calculated_mol_wt=20529
     CDS             1..184
                     /gene="LOC144115180"
                     /coded_by="XM_077649419.1:1231..1785"
                     /db_xref="GeneID:144115180"
ORIGIN      
        1 matckwhker lhesismpql glaprlalvl fligkkprlq eavmlaprad plvvvpkyrl
       61 nlsglftpnw sdapalldqg qgnhgcwcay rplaatqapw srrsvtleph rmainwkkrm
      121 scelclwahs cslkrasavh sshfsrlaac sgkgpttsde nwvlprwtrl lrgfrtgtrg
      181 epps
//
LOCUS       XP_077505546             184 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115180 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505546
VERSION     XP_077505546.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649420.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..184
                     /product="uncharacterized protein LOC144115180 isoform X1"
                     /calculated_mol_wt=20529
     CDS             1..184
                     /gene="LOC144115180"
                     /coded_by="XM_077649420.1:2296..2850"
                     /db_xref="GeneID:144115180"
ORIGIN      
        1 matckwhker lhesismpql glaprlalvl fligkkprlq eavmlaprad plvvvpkyrl
       61 nlsglftpnw sdapalldqg qgnhgcwcay rplaatqapw srrsvtleph rmainwkkrm
      121 scelclwahs cslkrasavh sshfsrlaac sgkgpttsde nwvlprwtrl lrgfrtgtrg
      181 epps
//
LOCUS       XP_077505549             184 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115180 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505549
VERSION     XP_077505549.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649423.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..184
                     /product="uncharacterized protein LOC144115180 isoform X1"
                     /calculated_mol_wt=20529
     CDS             1..184
                     /gene="LOC144115180"
                     /coded_by="XM_077649423.1:1419..1973"
                     /db_xref="GeneID:144115180"
ORIGIN      
        1 matckwhker lhesismpql glaprlalvl fligkkprlq eavmlaprad plvvvpkyrl
       61 nlsglftpnw sdapalldqg qgnhgcwcay rplaatqapw srrsvtleph rmainwkkrm
      121 scelclwahs cslkrasavh sshfsrlaac sgkgpttsde nwvlprwtrl lrgfrtgtrg
      181 epps
//
LOCUS       XP_077505550             184 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115180 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505550
VERSION     XP_077505550.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649424.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..184
                     /product="uncharacterized protein LOC144115180 isoform X1"
                     /calculated_mol_wt=20529
     CDS             1..184
                     /gene="LOC144115180"
                     /coded_by="XM_077649424.1:2245..2799"
                     /db_xref="GeneID:144115180"
ORIGIN      
        1 matckwhker lhesismpql glaprlalvl fligkkprlq eavmlaprad plvvvpkyrl
       61 nlsglftpnw sdapalldqg qgnhgcwcay rplaatqapw srrsvtleph rmainwkkrm
      121 scelclwahs cslkrasavh sshfsrlaac sgkgpttsde nwvlprwtrl lrgfrtgtrg
      181 epps
//
LOCUS       XP_077505551             184 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115180 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505551
VERSION     XP_077505551.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649425.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..184
                     /product="uncharacterized protein LOC144115180 isoform X1"
                     /calculated_mol_wt=20529
     CDS             1..184
                     /gene="LOC144115180"
                     /coded_by="XM_077649425.1:1314..1868"
                     /db_xref="GeneID:144115180"
ORIGIN      
        1 matckwhker lhesismpql glaprlalvl fligkkprlq eavmlaprad plvvvpkyrl
       61 nlsglftpnw sdapalldqg qgnhgcwcay rplaatqapw srrsvtleph rmainwkkrm
      121 scelclwahs cslkrasavh sshfsrlaac sgkgpttsde nwvlprwtrl lrgfrtgtrg
      181 epps
//
LOCUS       XP_077505552             184 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115180 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505552
VERSION     XP_077505552.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649426.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..184
                     /product="uncharacterized protein LOC144115180 isoform X1"
                     /calculated_mol_wt=20529
     CDS             1..184
                     /gene="LOC144115180"
                     /coded_by="XM_077649426.1:1427..1981"
                     /db_xref="GeneID:144115180"
ORIGIN      
        1 matckwhker lhesismpql glaprlalvl fligkkprlq eavmlaprad plvvvpkyrl
       61 nlsglftpnw sdapalldqg qgnhgcwcay rplaatqapw srrsvtleph rmainwkkrm
      121 scelclwahs cslkrasavh sshfsrlaac sgkgpttsde nwvlprwtrl lrgfrtgtrg
      181 epps
//
LOCUS       XP_077505553             184 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115180 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505553
VERSION     XP_077505553.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649427.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..184
                     /product="uncharacterized protein LOC144115180 isoform X1"
                     /calculated_mol_wt=20529
     CDS             1..184
                     /gene="LOC144115180"
                     /coded_by="XM_077649427.1:1255..1809"
                     /db_xref="GeneID:144115180"
ORIGIN      
        1 matckwhker lhesismpql glaprlalvl fligkkprlq eavmlaprad plvvvpkyrl
       61 nlsglftpnw sdapalldqg qgnhgcwcay rplaatqapw srrsvtleph rmainwkkrm
      121 scelclwahs cslkrasavh sshfsrlaac sgkgpttsde nwvlprwtrl lrgfrtgtrg
      181 epps
//
LOCUS       XP_077505554             184 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115180 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505554
VERSION     XP_077505554.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649428.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..184
                     /product="uncharacterized protein LOC144115180 isoform X1"
                     /calculated_mol_wt=20529
     CDS             1..184
                     /gene="LOC144115180"
                     /coded_by="XM_077649428.1:1289..1843"
                     /db_xref="GeneID:144115180"
ORIGIN      
        1 matckwhker lhesismpql glaprlalvl fligkkprlq eavmlaprad plvvvpkyrl
       61 nlsglftpnw sdapalldqg qgnhgcwcay rplaatqapw srrsvtleph rmainwkkrm
      121 scelclwahs cslkrasavh sshfsrlaac sgkgpttsde nwvlprwtrl lrgfrtgtrg
      181 epps
//
LOCUS       XP_077505555             184 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115180 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505555
VERSION     XP_077505555.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649429.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..184
                     /product="uncharacterized protein LOC144115180 isoform X1"
                     /calculated_mol_wt=20529
     CDS             1..184
                     /gene="LOC144115180"
                     /coded_by="XM_077649429.1:1206..1760"
                     /db_xref="GeneID:144115180"
ORIGIN      
        1 matckwhker lhesismpql glaprlalvl fligkkprlq eavmlaprad plvvvpkyrl
       61 nlsglftpnw sdapalldqg qgnhgcwcay rplaatqapw srrsvtleph rmainwkkrm
      121 scelclwahs cslkrasavh sshfsrlaac sgkgpttsde nwvlprwtrl lrgfrtgtrg
      181 epps
//
LOCUS       XP_077505556             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115180 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505556
VERSION     XP_077505556.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649430.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115180 isoform X2"
                     /calculated_mol_wt=20232
     CDS             1..181
                     /gene="LOC144115180"
                     /coded_by="XM_077649430.1:5800..6345"
                     /db_xref="GeneID:144115180"
ORIGIN      
        1 matckwhker lhesismpql glaprlalvl fligkkprlq eavmlaprad plvvvpkyrl
       61 nlsglftpnw sdapdqgqgn hgcwcayrpl aatqapwsrr svtlephrma inwkkrmsce
      121 lclwahscsl krasavhssh fsrlaacsgk gpttsdenwv lprwtrllrg frtgtrgepp
      181 s
//
LOCUS       XP_077505557             115 aa            linear   INV 25-NOV-2025
DEFINITION  argininosuccinate synthase-like [Amblyomma americanum].
ACCESSION   XP_077505557
VERSION     XP_077505557.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649431.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..115
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..115
                     /product="argininosuccinate synthase-like"
                     /calculated_mol_wt=12802
     Region          2..>69
                     /region_name="AANH_superfamily"
                     /note="Adenine nucleotide alpha hydrolase (AANH)
                     superfamily; cl00292"
                     /db_xref="CDD:469708"
     Region          5..>35
                     /region_name="ArgG"
                     /note="Argininosuccinate synthase [Amino acid transport
                     and metabolism]; COG0137"
                     /db_xref="CDD:439907"
     CDS             1..115
                     /gene="LOC144115181"
                     /coded_by="XM_077649431.1:56..403"
                     /db_xref="GeneID:144115181"
ORIGIN      
        1 mskptvvlay sggldtscil awlieqgyev vafmaggyni dfshyqllfe terkkhlkvf
       61 sklaaahvqr tnlqrmnmwl avdsselsal ftsmmtiprl yssarfigfc pcspp
//
LOCUS       XP_077505558             409 aa            linear   INV 25-NOV-2025
DEFINITION  argininosuccinate synthase [Amblyomma americanum].
ACCESSION   XP_077505558
VERSION     XP_077505558.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649432.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..409
                     /product="argininosuccinate synthase"
                     /calculated_mol_wt=45729
     Region          5..405
                     /region_name="ArgG"
                     /note="Argininosuccinate synthase [Amino acid transport
                     and metabolism]; COG0137"
                     /db_xref="CDD:439907"
     CDS             1..409
                     /gene="Ass"
                     /coded_by="XM_077649432.1:324..1553"
                     /db_xref="GeneID:144115182"
ORIGIN      
        1 mskptvvlay sggldtscil awlieqgyev vafmanvgqe edfvaaeaka krigakkvlv
       61 edlrrefvee fvfpalraga vyeeryllgt alarpcigra laeaaarega afvshgatgk
      121 gndqirfela csalrpsvqt iapwrmpefy krfqgrqdlf ayakehgiev pvtpkapwsm
      181 danlmhvsye sgilenpesp apeeiylmtr dprkapeepe tleiefengk pvkvtnvsng
      241 vqktdslelf lylneiggrh gigridiven rvigmksrgv yetpagtvlr aahldlevfa
      301 mdkevrrikq glslefsrqv yaglwfspea eftrkciems qscvtgrvrl dlyrgqvyik
      361 grwapnslyn qelvsmdvqg dytpsdaggf ikiqalrlre whrvkiaqq
//
LOCUS       XP_077505559             409 aa            linear   INV 25-NOV-2025
DEFINITION  argininosuccinate synthase [Amblyomma americanum].
ACCESSION   XP_077505559
VERSION     XP_077505559.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649433.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..409
                     /product="argininosuccinate synthase"
                     /calculated_mol_wt=45729
     Region          5..405
                     /region_name="ArgG"
                     /note="Argininosuccinate synthase [Amino acid transport
                     and metabolism]; COG0137"
                     /db_xref="CDD:439907"
     CDS             1..409
                     /gene="Ass"
                     /coded_by="XM_077649433.1:318..1547"
                     /db_xref="GeneID:144115182"
ORIGIN      
        1 mskptvvlay sggldtscil awlieqgyev vafmanvgqe edfvaaeaka krigakkvlv
       61 edlrrefvee fvfpalraga vyeeryllgt alarpcigra laeaaarega afvshgatgk
      121 gndqirfela csalrpsvqt iapwrmpefy krfqgrqdlf ayakehgiev pvtpkapwsm
      181 danlmhvsye sgilenpesp apeeiylmtr dprkapeepe tleiefengk pvkvtnvsng
      241 vqktdslelf lylneiggrh gigridiven rvigmksrgv yetpagtvlr aahldlevfa
      301 mdkevrrikq glslefsrqv yaglwfspea eftrkciems qscvtgrvrl dlyrgqvyik
      361 grwapnslyn qelvsmdvqg dytpsdaggf ikiqalrlre whrvkiaqq
//
LOCUS       XP_077505560             272 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115183 [Amblyomma americanum].
ACCESSION   XP_077505560
VERSION     XP_077505560.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649434.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..272
                     /product="uncharacterized protein LOC144115183"
                     /calculated_mol_wt=30229
     Region          <105..166
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..272
                     /gene="LOC144115183"
                     /coded_by="XM_077649434.1:139..957"
                     /db_xref="GeneID:144115183"
ORIGIN      
        1 mgafpgaprg ksgsegdlps prhhprrrrl hrrhadchqs aagrpskpgs ilepqrilrd
       61 ehhdhlrrrh edpcgrpslp gvlsrclfla llaaaseggh glledgefll gdsgyplepw
      121 lltpvsghpg antaegqyna ahasmrcvve rcigvlksrf rclqryrtlh yeperavvii
      181 aacaalhnic leealaaded dttddpdnng ppllagylag tgsrmtylrg ramrdriigl
      241 fgttrpqrqa hlqmvrrqqq rqqqrqlqhh rq
//
LOCUS       XP_077505561             163 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115184 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505561
VERSION     XP_077505561.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649435.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..163
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..163
                     /product="uncharacterized protein LOC144115184 isoform X1"
                     /calculated_mol_wt=18381
     CDS             1..163
                     /gene="LOC144115184"
                     /coded_by="XM_077649435.1:229..720"
                     /db_xref="GeneID:144115184"
ORIGIN      
        1 mscsaastlp psssrcskki lmflltylkt skfgtgkksv sedcqvriad vldepqqaeq
       61 srvtatyslt yslpavpndi qfsierhqsg qyfkarsrvv qwlyhdlcly tmtlgeklsv
      121 srqnmsgeks vssrmmgqil hrqknqrkmg kwprlaempc svf
//
LOCUS       XP_077505562             122 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115184 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505562
VERSION     XP_077505562.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649436.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..122
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..122
                     /product="uncharacterized protein LOC144115184 isoform X2"
                     /calculated_mol_wt=13642
     CDS             1..122
                     /gene="LOC144115184"
                     /coded_by="XM_077649436.1:229..597"
                     /db_xref="GeneID:144115184"
ORIGIN      
        1 mscsaastlp psssrcskki lmflltylkt skfgtgkksv sedcqvriad vldepqqaeq
       61 srvtatyslt yslpavpndi qfsierhqsg qyfkarsrvv qwlyhdlcly tmypgklyne
      121 aa
//
LOCUS       XP_077505563             774 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505563
VERSION     XP_077505563.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649437.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..774
                     /product="nose resistant to fluoxetine protein 6-like
                     isoform X1"
                     /calculated_mol_wt=85494
     Region          104..228
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          319..732
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..774
                     /gene="LOC144115185"
                     /coded_by="XM_077649437.1:85..2409"
                     /db_xref="GeneID:144115185"
ORIGIN      
        1 mrtspaallr palrplllll llspllvpas gaqdaepass asaasgaaad dalesdeqtk
       61 dtesmtrqws ameaalrsta eqavqtmfpa lvkvsgeael sgdcqaallk vilgvrrlrd
      121 wairladssg klpmgvmtgs latlgsfdqc lsvhhdegdg dpivgryctl niqfpglprp
      181 fknsselikd pesfmhwvyn ygkwikyfpi rigvclpshc ekedleklgr hvvrrakmks
      241 wvgtcevdgp rkvntiqlvv msilgilavc vglgtlvdrf tgprdeskkl rgdetplefa
      301 relalsfsfl antrrllqpa rrnsslgvlh girvvsclwi ilghtyflgd ittfvrfkrl
      361 ktleelntdv pftaienflp vdtfffisgl livysnwpri ekkkgklnvl sslfqrfwrm
      421 tpsyllvlgs fsilpllgsg plwhevmtpl mnncanswws nllyvsnylp ydkmcllhtw
      481 fqavnmqffv lalplmlfiy kfaalglalv vtlclascla vglityffdl ppalllmtse
      541 wskavqqlnl lyfqpfthfg pfcvgllcgy ylvknktitm srptlwlgwv callcglvsl
      601 wgvypfragf dvhpalvscy aalhrtiwca avawlvvach vghgglintt lawnglvpls
      661 tlsyliylih plviymhvat areivnldqf smcysflght avsvllayva hvtvelpfat
      721 vkgllmqrrr aslslaakgr qaplqaivcg avavnghrpt aapqpgfghv tcna
//
LOCUS       XP_077505564             641 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505564
VERSION     XP_077505564.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649438.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..641
                     /product="nose resistant to fluoxetine protein 6-like
                     isoform X2"
                     /calculated_mol_wt=71586
     Region          <2..95
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          186..599
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..641
                     /gene="LOC144115185"
                     /coded_by="XM_077649438.1:276..2201"
                     /db_xref="GeneID:144115185"
ORIGIN      
        1 mgvmtgslat lgsfdqclsv hhdegdgdpi vgryctlniq fpglprpfkn sselikdpes
       61 fmhwvynygk wikyfpirig vclpshceke dleklgrhvv rrakmkswvg tcevdgprkv
      121 ntiqlvvmsi lgilavcvgl gtlvdrftgp rdeskklrgd etplefarel alsfsflant
      181 rrllqparrn sslgvlhgir vvsclwiilg htyflgditt fvrfkrlktl eelntdvpft
      241 aienflpvdt fffisglliv ysnwpriekk kgklnvlssl fqrfwrmtps yllvlgsfsi
      301 lpllgsgplw hevmtplmnn canswwsnll yvsnylpydk mcllhtwfqa vnmqffvlal
      361 plmlfiykfa alglalvvtl clasclavgl ityffdlppa lllmtsewsk avqqlnllyf
      421 qpfthfgpfc vgllcgyylv knktitmsrp tlwlgwvcal lcglvslwgv ypfragfdvh
      481 palvscyaal hrtiwcaava wlvvachvgh gglinttlaw nglvplstls yliylihplv
      541 iymhvatare ivnldqfsmc ysflghtavs vllayvahvt velpfatvkg llmqrrrasl
      601 slaakgrqap lqaivcgava vnghrptaap qpgfghvtcn a
//
LOCUS       XP_077505565             484 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115186 [Amblyomma americanum].
ACCESSION   XP_077505565
VERSION     XP_077505565.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649439.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..484
                     /product="uncharacterized protein LOC144115186"
                     /calculated_mol_wt=55347
     Region          <165..286
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     CDS             1..484
                     /gene="LOC144115186"
                     /coded_by="XM_077649439.1:430..1884"
                     /db_xref="GeneID:144115186"
ORIGIN      
        1 mwdicdrssd qeansdgygs eletflyssi yhdaqeeasl peasptaisn sdadapvltv
       61 vqsrkrrada eelkcvvkkv krskmralaa laaepkpsas ptadcillss dddevmvfse
      121 dsqerialnv vetsqdkenk sglwhvdaed lyrdsrgfry hhtlanihcr ncdqmghlsk
      181 hctqpkinvc pfcselghda krcpqrmcsr cymqghvlae ckqtfvptcs lcntkghpde
      241 rcpdlwrryh mttkdgpivr arvkmrpree rycfncgrqg hyghqchqrk hgnpstpyii
      301 sykdpfrpeq pplvlrdekr kqrvdkkrrl kneqlqkwle tvqcngqatp rhkktrhgkk
      361 rqapldhgqt sgmvqngeta qserppsepr graagncegq qatpgapaak rqkkspakaa
      421 msksawrrrk lrekkkkare qqkqqqmknp yqgeesnygq cstfdnpsfy mpnnwcnktf
      481 wgyy
//
LOCUS       XP_077505566             481 aa            linear   INV 25-NOV-2025
DEFINITION  germ cell-less isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505566
VERSION     XP_077505566.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649440.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..481
                     /product="germ cell-less isoform X1"
                     /calculated_mol_wt=54603
     Region          48..162
                     /region_name="BTB_POZ_GCL"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster protein germ cell-less (GCL) and similar
                     proteins; cd18305"
                     /db_xref="CDD:349614"
     Region          163..242
                     /region_name="BACK_GCL"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     Drosophila melanogaster protein germ cell-less (GCL) and
                     similar proteins; cd18495"
                     /db_xref="CDD:350570"
     CDS             1..481
                     /gene="gcl"
                     /coded_by="XM_077649440.1:351..1796"
                     /db_xref="GeneID:144115187"
ORIGIN      
        1 mgnvlltssl gtaatslakr krksaeddss eensewgsap krrrlmttsq yiyrtlfleg
       61 qnsdvtival gvdwclhkvy lcqspyfasm fcgdwkekee gtvevgiedp hitvdalkiv
      121 fgslyldeia ispedvipvl asatlfqlde lielcseimg etieldtvlp yyevaaqygl
      181 tqlkkkcfew lernlmflvn enpkilrqic pslmlqlves pslvviqtef slyllismwa
      241 flrihasweg direcsqlsh eyfqqrrgel taegsftqse tgreyadvfq alriehlinh
      301 pldvamveed nifskeslyp vfrnqwfsml ridqgldkgp knlaeeefnk rclrcgrvla
      361 tdaqhiwrwt gynfgvdliv tyhgrtikfr rndrsepdvl rsiaawgrrh llyrvsvyta
      421 gpfgdallla ssglrsatla rnaetkvlti esevklpiii sanflvttpl dppetpespq
      481 s
//
LOCUS       XP_077505567             480 aa            linear   INV 25-NOV-2025
DEFINITION  germ cell-less isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505567
VERSION     XP_077505567.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649441.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..480
                     /product="germ cell-less isoform X2"
                     /calculated_mol_wt=54532
     Region          48..162
                     /region_name="BTB_POZ_GCL"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster protein germ cell-less (GCL) and similar
                     proteins; cd18305"
                     /db_xref="CDD:349614"
     Region          163..242
                     /region_name="BACK_GCL"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     Drosophila melanogaster protein germ cell-less (GCL) and
                     similar proteins; cd18495"
                     /db_xref="CDD:350570"
     CDS             1..480
                     /gene="gcl"
                     /coded_by="XM_077649441.1:351..1793"
                     /db_xref="GeneID:144115187"
ORIGIN      
        1 mgnvlltssl gtaatslakr krksaeddss eensewgsap krrrlmttsq yiyrtlfleg
       61 qnsdvtival gvdwclhkvy lcqspyfasm fcgdwkekee gtvevgiedp hitvdalkiv
      121 fgslyldeia ispedvipvl asatlfqlde lielcseimg etieldtvlp yyevaaqygl
      181 tqlkkkcfew lernlmflvn enpkilrqic pslmlqlves pslvviqtef slyllismwa
      241 flrihasweg direcsqlsh eyfqqrrgel tegsftqset greyadvfqa lriehlinhp
      301 ldvamveedn ifskeslypv frnqwfsmlr idqgldkgpk nlaeeefnkr clrcgrvlat
      361 daqhiwrwtg ynfgvdlivt yhgrtikfrr ndrsepdvlr siaawgrrhl lyrvsvytag
      421 pfgdalllas sglrsatlar naetkvltie sevklpiiis anflvttpld ppetpespqs
//
LOCUS       XP_077505568             477 aa            linear   INV 25-NOV-2025
DEFINITION  germ cell-less isoform X3 [Amblyomma americanum].
ACCESSION   XP_077505568
VERSION     XP_077505568.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649442.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..477
                     /product="germ cell-less isoform X3"
                     /calculated_mol_wt=54202
     Region          48..162
                     /region_name="BTB_POZ_GCL"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster protein germ cell-less (GCL) and similar
                     proteins; cd18305"
                     /db_xref="CDD:349614"
     Region          163..242
                     /region_name="BACK_GCL"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     Drosophila melanogaster protein germ cell-less (GCL) and
                     similar proteins; cd18495"
                     /db_xref="CDD:350570"
     CDS             1..477
                     /gene="gcl"
                     /coded_by="XM_077649442.1:348..1781"
                     /db_xref="GeneID:144115187"
ORIGIN      
        1 mgnvlltssl gtaatslakr krksaeddss eensewgsap krrrlmttsq yiyrtlfleg
       61 qnsdvtival gvdwclhkvy lcqspyfasm fcgdwkekee gtvevgiedp hitvdalkiv
      121 fgslyldeia ispedvipvl asatlfqlde lielcseimg etieldtvlp yyevaaqygl
      181 tqlkkkcfew lernlmflvn enpkilrqic pslmlqlves pslvviqtef slyllismwa
      241 flrihasweg direcsqlsh eyfqqrraeg sftqsetgre yadvfqalri ehlinhpldv
      301 amveednifs keslypvfrn qwfsmlridq gldkgpknla eeefnkrclr cgrvlatdaq
      361 hiwrwtgynf gvdlivtyhg rtikfrrndr sepdvlrsia awgrrhllyr vsvytagpfg
      421 dalllassgl rsatlarnae tkvltiesev klpiiisanf lvttpldppe tpespqs
//
LOCUS       XP_077505569             476 aa            linear   INV 25-NOV-2025
DEFINITION  germ cell-less isoform X4 [Amblyomma americanum].
ACCESSION   XP_077505569
VERSION     XP_077505569.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649443.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..476
                     /product="germ cell-less isoform X4"
                     /calculated_mol_wt=54131
     Region          48..162
                     /region_name="BTB_POZ_GCL"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster protein germ cell-less (GCL) and similar
                     proteins; cd18305"
                     /db_xref="CDD:349614"
     Region          163..242
                     /region_name="BACK_GCL"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     Drosophila melanogaster protein germ cell-less (GCL) and
                     similar proteins; cd18495"
                     /db_xref="CDD:350570"
     CDS             1..476
                     /gene="gcl"
                     /coded_by="XM_077649443.1:348..1778"
                     /db_xref="GeneID:144115187"
ORIGIN      
        1 mgnvlltssl gtaatslakr krksaeddss eensewgsap krrrlmttsq yiyrtlfleg
       61 qnsdvtival gvdwclhkvy lcqspyfasm fcgdwkekee gtvevgiedp hitvdalkiv
      121 fgslyldeia ispedvipvl asatlfqlde lielcseimg etieldtvlp yyevaaqygl
      181 tqlkkkcfew lernlmflvn enpkilrqic pslmlqlves pslvviqtef slyllismwa
      241 flrihasweg direcsqlsh eyfqqrregs ftqsetgrey advfqalrie hlinhpldva
      301 mveednifsk eslypvfrnq wfsmlridqg ldkgpknlae eefnkrclrc grvlatdaqh
      361 iwrwtgynfg vdlivtyhgr tikfrrndrs epdvlrsiaa wgrrhllyrv svytagpfgd
      421 alllassglr satlarnaet kvltiesevk lpiiisanfl vttpldppet pespqs
//
LOCUS       XP_077505570             390 aa            linear   INV 25-NOV-2025
DEFINITION  inorganic pyrophosphatase Nurf-38 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505570
VERSION     XP_077505570.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649444.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..390
                     /product="inorganic pyrophosphatase Nurf-38 isoform X1"
                     /calculated_mol_wt=43708
     Region          106..338
                     /region_name="pyrophosphatase"
                     /note="Inorganic pyrophosphatase. These enzymes hydrolyze
                     inorganic pyrophosphate (PPi) to two molecules of
                     orthophosphates (Pi). The reaction requires bivalent
                     cations. The enzymes in general exist as homooligomers;
                     cl00217"
                     /db_xref="CDD:469666"
     Site            order(156..157,232..233)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:238239"
     Site            order(161,183,198,297..298)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:238239"
     Site            order(163,220,225,252,257)
                     /site_type="metal-binding"
                     /note="metal binding sites [ion binding]"
                     /db_xref="CDD:238239"
     CDS             1..390
                     /gene="Nurf-38"
                     /coded_by="XM_077649444.1:237..1409"
                     /db_xref="GeneID:144115189"
ORIGIN      
        1 mlfqgaiglt villlsqvpp lwqpasemll hvgkafttgf lrlrgvsfsl anvsrtmays
       61 tvergspntl tyklyfrkll tacgpvlfsm mgdflvlaas gmaldsystv ergspnsany
      121 smyfrkgdqy ispfhdipmf adesnriynm vvevprwtna kmeiatkepl npikqdvkkg
      181 klryvhncfp hhgyiwnyga ipqtwedpnh tdsktnckgd ndpidvceig yrvakrgevl
      241 qvkilgvmal vdegetdwkl laidvkdpla nelndvadie khmpgllrat newfriykip
      301 dgkpenqfaf ngeaknrefa ekviaethgf wlalmqrkdt splncstvqs gsehyisdee
      361 assivnstpe lgpdagmdpi vdkwhfvslk
//
LOCUS       XP_077505571             342 aa            linear   INV 25-NOV-2025
DEFINITION  inorganic pyrophosphatase Nurf-38 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505571
VERSION     XP_077505571.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649445.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..342
                     /product="inorganic pyrophosphatase Nurf-38 isoform X2"
                     /calculated_mol_wt=38555
     Region          35..290
                     /region_name="pyrophosphatase"
                     /note="Inorganic pyrophosphatase. These enzymes hydrolyze
                     inorganic pyrophosphate (PPi) to two molecules of
                     orthophosphates (Pi). The reaction requires bivalent
                     cations. The enzymes in general exist as homooligomers;
                     cl00217"
                     /db_xref="CDD:469666"
     Site            order(108..109,184..185)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:238239"
     Site            order(113,135,150,249..250)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:238239"
     Site            order(115,172,177,204,209)
                     /site_type="metal-binding"
                     /note="metal binding sites [ion binding]"
                     /db_xref="CDD:238239"
     CDS             1..342
                     /gene="Nurf-38"
                     /coded_by="XM_077649445.1:230..1258"
                     /db_xref="GeneID:144115189"
ORIGIN      
        1 mlfqgaiglt villlsqvpp lwqpasemll hvgkafttgf lrlrgvsfsl anvsrtmays
       61 tvergspntl tyklyfrkgd qyispfhdip mfadesnriy nmvvevprwt nakmeiatke
      121 plnpikqdvk kgklryvhnc fphhgyiwny gaipqtwedp nhtdsktnck gdndpidvce
      181 igyrvakrge vlqvkilgvm alvdegetdw kllaidvkdp lanelndvad iekhmpgllr
      241 atnewfriyk ipdgkpenqf afngeaknre faekviaeth gfwlalmqrk dtsplncstv
      301 qsgsehyisd eeassivnst pelgpdagmd pivdkwhfvs lk
//
LOCUS       XP_077505572             293 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115190 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505572
VERSION     XP_077505572.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649446.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..293
                     /product="uncharacterized protein LOC144115190 isoform X1"
                     /calculated_mol_wt=32590
     CDS             1..293
                     /gene="LOC144115190"
                     /coded_by="XM_077649446.1:436..1317"
                     /db_xref="GeneID:144115190"
ORIGIN      
        1 mdtaaleqns tgpmectwrk kddarvtard calgtadilt wrvdevvarg csctdvrvlr
       61 dtkerlstsl ldslehskma tckrhkghlh esiwtpqlgl aprlalllfl igkkprlqea
      121 vmlapqadpv vvvpkyrlnl fligkkprlq eavmlaprad pvvvvpkyrl nlsglftpnw
      181 sdapalldqr lgnhgcwcay rplaatqapw srrsatleph rmainwkrma cecclwvhsc
      241 sskrasavrs shvsrlpacg gkgpttsden wglprwtrll rgyrtgtrge pps
//
LOCUS       XP_077505573             293 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115190 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505573
VERSION     XP_077505573.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649447.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..293
                     /product="uncharacterized protein LOC144115190 isoform X1"
                     /calculated_mol_wt=32590
     CDS             1..293
                     /gene="LOC144115190"
                     /coded_by="XM_077649447.1:823..1704"
                     /db_xref="GeneID:144115190"
ORIGIN      
        1 mdtaaleqns tgpmectwrk kddarvtard calgtadilt wrvdevvarg csctdvrvlr
       61 dtkerlstsl ldslehskma tckrhkghlh esiwtpqlgl aprlalllfl igkkprlqea
      121 vmlapqadpv vvvpkyrlnl fligkkprlq eavmlaprad pvvvvpkyrl nlsglftpnw
      181 sdapalldqr lgnhgcwcay rplaatqapw srrsatleph rmainwkrma cecclwvhsc
      241 sskrasavrs shvsrlpacg gkgpttsden wglprwtrll rgyrtgtrge pps
//
LOCUS       XP_077505574             293 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115190 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505574
VERSION     XP_077505574.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649448.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..293
                     /product="uncharacterized protein LOC144115190 isoform X1"
                     /calculated_mol_wt=32590
     CDS             1..293
                     /gene="LOC144115190"
                     /coded_by="XM_077649448.1:455..1336"
                     /db_xref="GeneID:144115190"
ORIGIN      
        1 mdtaaleqns tgpmectwrk kddarvtard calgtadilt wrvdevvarg csctdvrvlr
       61 dtkerlstsl ldslehskma tckrhkghlh esiwtpqlgl aprlalllfl igkkprlqea
      121 vmlapqadpv vvvpkyrlnl fligkkprlq eavmlaprad pvvvvpkyrl nlsglftpnw
      181 sdapalldqr lgnhgcwcay rplaatqapw srrsatleph rmainwkrma cecclwvhsc
      241 sskrasavrs shvsrlpacg gkgpttsden wglprwtrll rgyrtgtrge pps
//
LOCUS       XP_077505575             293 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115190 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505575
VERSION     XP_077505575.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649449.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..293
                     /product="uncharacterized protein LOC144115190 isoform X1"
                     /calculated_mol_wt=32590
     CDS             1..293
                     /gene="LOC144115190"
                     /coded_by="XM_077649449.1:363..1244"
                     /db_xref="GeneID:144115190"
ORIGIN      
        1 mdtaaleqns tgpmectwrk kddarvtard calgtadilt wrvdevvarg csctdvrvlr
       61 dtkerlstsl ldslehskma tckrhkghlh esiwtpqlgl aprlalllfl igkkprlqea
      121 vmlapqadpv vvvpkyrlnl fligkkprlq eavmlaprad pvvvvpkyrl nlsglftpnw
      181 sdapalldqr lgnhgcwcay rplaatqapw srrsatleph rmainwkrma cecclwvhsc
      241 sskrasavrs shvsrlpacg gkgpttsden wglprwtrll rgyrtgtrge pps
//
LOCUS       XP_077505576             293 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115190 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505576
VERSION     XP_077505576.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649450.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..293
                     /product="uncharacterized protein LOC144115190 isoform X1"
                     /calculated_mol_wt=32590
     CDS             1..293
                     /gene="LOC144115190"
                     /coded_by="XM_077649450.1:337..1218"
                     /db_xref="GeneID:144115190"
ORIGIN      
        1 mdtaaleqns tgpmectwrk kddarvtard calgtadilt wrvdevvarg csctdvrvlr
       61 dtkerlstsl ldslehskma tckrhkghlh esiwtpqlgl aprlalllfl igkkprlqea
      121 vmlapqadpv vvvpkyrlnl fligkkprlq eavmlaprad pvvvvpkyrl nlsglftpnw
      181 sdapalldqr lgnhgcwcay rplaatqapw srrsatleph rmainwkrma cecclwvhsc
      241 sskrasavrs shvsrlpacg gkgpttsden wglprwtrll rgyrtgtrge pps
//
LOCUS       XP_077505577             290 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115190 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505577
VERSION     XP_077505577.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649451.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..290
                     /product="uncharacterized protein LOC144115190 isoform X2"
                     /calculated_mol_wt=32238
     CDS             1..290
                     /gene="LOC144115190"
                     /coded_by="XM_077649451.1:436..1308"
                     /db_xref="GeneID:144115190"
ORIGIN      
        1 mdtaaleqns tgpmectwrk kddarvtard calgtadilt wrvdevvarg csctdvrvlr
       61 dtkerlstsl ldslematck rhkghlhesi wtpqlglapr lalllfligk kprlqeavml
      121 apqadpvvvv pkyrlnlfli gkkprlqeav mlapradpvv vvpkyrlnls glftpnwsda
      181 palldqrlgn hgcwcayrpl aatqapwsrr satlephrma inwkrmacec clwvhscssk
      241 rasavrsshv srlpacggkg pttsdenwgl prwtrllrgy rtgtrgepps
//
LOCUS       XP_077505578             270 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115190 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505578
VERSION     XP_077505578.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649452.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..270
                     /product="uncharacterized protein LOC144115190 isoform X3"
                     /calculated_mol_wt=29730
     CDS             1..270
                     /gene="LOC144115190"
                     /coded_by="XM_077649452.1:436..1248"
                     /db_xref="GeneID:144115190"
ORIGIN      
        1 mdtaaleqns tgpmectwrk kddarvtard calgtadilt wrvdevvarg csctdvrvlr
       61 dtkerlstsl ldslehskma tckrhkghlh esiwtpqlgl aprlalllfl igkkprlqea
      121 vmlapqadpv vvvpkyrlnl fligkkprlq eavmlaprad pvvvvpkyrl nlsglftpnw
      181 sdapellsfa rpatrqpwll vcvssfgghp sslepqecdp gatshghqle knglrvlsvg
      241 allqleacic gafeprftsa clrregaynf
//
LOCUS       XP_077505579             220 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115190 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505579
VERSION     XP_077505579.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649453.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..220
                     /product="uncharacterized protein LOC144115190 isoform X4"
                     /calculated_mol_wt=24520
     CDS             1..220
                     /gene="LOC144115190"
                     /coded_by="XM_077649453.1:417..1079"
                     /db_xref="GeneID:144115190"
ORIGIN      
        1 mqhskmatck rhkghlhesi wtpqlglapr lalllfligk kprlqeavml apqadpvvvv
       61 pkyrlnlfli gkkprlqeav mlapradpvv vvpkyrlnls glftpnwsda palldqrlgn
      121 hgcwcayrpl aatqapwsrr satlephrma inwkrmacec clwvhscssk rasavrsshv
      181 srlpacggkg pttsdenwgl prwtrllrgy rtgtrgepps
//
LOCUS       XP_077505580             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115190 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077505580
VERSION     XP_077505580.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649454.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144115190 isoform X5"
                     /calculated_mol_wt=24167
     CDS             1..217
                     /gene="LOC144115190"
                     /coded_by="XM_077649454.1:417..1070"
                     /db_xref="GeneID:144115190"
ORIGIN      
        1 mqmatckrhk ghlhesiwtp qlglaprlal llfligkkpr lqeavmlapq adpvvvvpky
       61 rlnlfligkk prlqeavmla pradpvvvvp kyrlnlsglf tpnwsdapal ldqrlgnhgc
      121 wcayrplaat qapwsrrsat lephrmainw krmacecclw vhscsskras avrsshvsrl
      181 pacggkgptt sdenwglprw trllrgyrtg trgepps
//
LOCUS       XP_077505581             202 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115192 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505581
VERSION     XP_077505581.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649455.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..202
                     /product="uncharacterized protein LOC144115192 isoform X1"
                     /calculated_mol_wt=22233
     CDS             1..202
                     /gene="LOC144115192"
                     /coded_by="XM_077649455.1:3609..4217"
                     /db_xref="GeneID:144115192"
ORIGIN      
        1 matckwhkgr lhesiwtlql glaprlalll fligkkprlq eavmlvprad pvvvvpkyrl
       61 nlsglftpnw sdapgkkewp vsvvcgrtpa arsvhlrcvr ttfhvclpam grglqlltkt
      121 gsspcwtaav awlpysdqrg gaivaacssq qllaaafcha nsrnlqprmt ssyrlcqaqc
      181 tvhlentfpr tplaatwffs ei
//
LOCUS       XP_077505582             130 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115192 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505582
VERSION     XP_077505582.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649456.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..130
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..130
                     /product="uncharacterized protein LOC144115192 isoform X2"
                     /calculated_mol_wt=14274
     CDS             1..130
                     /gene="LOC144115192"
                     /coded_by="XM_077649456.1:3607..3999"
                     /db_xref="GeneID:144115192"
ORIGIN      
        1 matckwhkgr lhesiwtlql glaprlalll fligkkprlq eavmlvprad pvvvvpkyrl
       61 nlsglftpnw sdapalldqg qgnhgcwcay rplaatqapw srrsatleph rmainwrvrs
      121 pagcntasgg
//
LOCUS       XP_077505584             622 aa            linear   INV 25-NOV-2025
DEFINITION  gamma-glutamyl carboxylase [Amblyomma americanum].
ACCESSION   XP_077505584
VERSION     XP_077505584.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649458.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..622
                     /product="gamma-glutamyl carboxylase"
                     /calculated_mol_wt=72084
     Region          1..439
                     /region_name="VKG_Carbox"
                     /note="Vitamin K-dependent gamma-carboxylase; pfam05090"
                     /db_xref="CDD:461546"
     CDS             1..622
                     /gene="GC"
                     /coded_by="XM_077649458.1:147..2015"
                     /db_xref="GeneID:144115193"
ORIGIN      
        1 mvldqpeerg lafveerwgd precrfplfs flkplplegm ycvqllmllg avgittgaff
       61 rksclafllp ywfiflleks rwnnhtylyg liamllsvtg ahrlwsidcw rkqflrntdi
      121 prwnyvllrs qvflvyfiag lkktdrdwig ghsmeglgrh wvfdlfklvl teeqvdsyvv
      181 hwggflldlt vgflmvasav rpfgvlfcvl fnamnsrmfs igmfpyvmiv lstiffdcsw
      241 pkklsaalpq riknlipdlc aprpdmnncw ypaqskgndp cagsrwaerr lrrwrlttff
      301 lclhlalqlf lpyshgltkg yntwtqgsyg yswdmmihkw rpvhvkllly dnathkahfl
      361 dpekftnstr whhqadmvvq fahcirnrvr qefgmrdvei yvdvwmslng rfaqrmydpt
      421 vnileqpwsp fsevpwvlpv ltnfgewren fkkvrsevqa sstladvefi adfpgfslvn
      481 yldttvynas levlkgtvai sfpskrstrv lktgestkip tgvyhtvtpl khapagymyl
      541 fadksleqnn tgtegdadsl pentaakkei isirhlkryl enwrisfrri adiagktlkk
      601 rllhkissll erflakcslc kp
//
LOCUS       XP_077505585             715 aa            linear   INV 25-NOV-2025
DEFINITION  long-chain-fatty-acid--CoA ligase 5 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505585
VERSION     XP_077505585.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649459.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..715
                     /product="long-chain-fatty-acid--CoA ligase 5 isoform X1"
                     /calculated_mol_wt=79117
     Region          136..712
                     /region_name="LC-FACS_euk"
                     /note="Eukaryotic long-chain fatty acid CoA synthetase
                     (LC-FACS); cd05927"
                     /db_xref="CDD:341250"
     Site            order(291,294..299,301..302)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341250"
     Site            order(294,411..412,474..479,558,570,573,585,691)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341250"
     Site            order(294,337..338,383,385..386,389,411..412,474..479,558,
                     570,573,582..585,668)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341250"
     Site            order(337,385..386,389,411,582..584,666,668)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341250"
     CDS             1..715
                     /gene="LOC144115194"
                     /coded_by="XM_077649459.1:377..2524"
                     /db_xref="GeneID:144115194"
ORIGIN      
        1 madtgagqdq ksddkagark ttevakkrps lfgmseylsy vggvhglaaa agaaaalglt
       61 ymfgrsanti eptvsldnqs rlltglerir tsrlcpdgnh ieylyedcrt lfdvmyrgar
      121 vsnngpcmgw rvpgsgeykw ihynqvleri kciacglvkl gekpgqqtfi glytqncvew
      181 ivteqacyyf smiivplydt lgaaacafii nqadietvvc dtqekvecll rqagetpklk
      241 riiviknvse dvkekarnan imlmkfsele elgeknfrhp lpphttdlat vcytsgttgn
      301 pkgvmlthln iisdlsavmr qlgdvaptgd dvmlsflpla hmlerccema iymiggkvgf
      361 fsgdikelge dmkalrptis ptvprllnri hdkvisascg sffkrtllnl alkskeaelq
      421 nfivrrnglw drlvfksvhe smggrlrlmv vgsaplapsv itfvrcalgc vivegygqte
      481 ctapctltfp gdynaghvgp pigacnvklv dvpdmeyyas nnegeicvkg ptvfqgylkd
      541 pektaevldk dgwlhtgdig kwldngclkv idrkkhifkl sqgeyiapek lenifirslf
      601 vaqifvhges lksclvavvi pdqeylmqwa rensisgtfq elcrnnivkk silddlsrlg
      661 klaglksfel lkdiylhsdv lsvdnglltp tlktkrpecr kyflsqienm yrsla
//
LOCUS       XP_077505586             699 aa            linear   INV 25-NOV-2025
DEFINITION  long-chain-fatty-acid--CoA ligase 5 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505586
VERSION     XP_077505586.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649460.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..699
                     /product="long-chain-fatty-acid--CoA ligase 5 isoform X2"
                     /calculated_mol_wt=77461
     Region          120..696
                     /region_name="LC-FACS_euk"
                     /note="Eukaryotic long-chain fatty acid CoA synthetase
                     (LC-FACS); cd05927"
                     /db_xref="CDD:341250"
     Site            order(275,278..283,285..286)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341250"
     Site            order(278,395..396,458..463,542,554,557,569,675)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341250"
     Site            order(278,321..322,367,369..370,373,395..396,458..463,542,
                     554,557,566..569,652)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341250"
     Site            order(321,369..370,373,395,566..568,650,652)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341250"
     CDS             1..699
                     /gene="LOC144115194"
                     /coded_by="XM_077649460.1:215..2314"
                     /db_xref="GeneID:144115194"
ORIGIN      
        1 mvvhsnplkn lgpslfgmse ylsyvggvhg laaaagaaaa lgltymfgrs antieptvsl
       61 dnqsrlltgl erirtsrlcp dgnhieylye dcrtlfdvmy rgarvsnngp cmgwrvpgsg
      121 eykwihynqv lerikciacg lvklgekpgq qtfiglytqn cvewivteqa cyyfsmiivp
      181 lydtlgaaac afiinqadie tvvcdtqekv ecllrqaget pklkriivik nvsedvkeka
      241 rnanimlmkf seleelgekn frhplpphtt dlatvcytsg ttgnpkgvml thlniisdls
      301 avmrqlgdva ptgddvmlsf lplahmlerc cemaiymigg kvgffsgdik elgedmkalr
      361 ptisptvprl lnrihdkvis ascgsffkrt llnlalkske aelqnfivrr nglwdrlvfk
      421 svhesmggrl rlmvvgsapl apsvitfvrc algcvivegy gqtectapct ltfpgdynag
      481 hvgppigacn vklvdvpdme yyasnnegei cvkgptvfqg ylkdpektae vldkdgwlht
      541 gdigkwldng clkvidrkkh ifklsqgeyi apeklenifi rslfvaqifv hgeslksclv
      601 avvipdqeyl mqwarensis gtfqelcrnn ivkksilddl srlgklaglk sfellkdiyl
      661 hsdvlsvdng lltptlktkr pecrkyflsq ienmyrsla
//
LOCUS       XP_077505587             682 aa            linear   INV 25-NOV-2025
DEFINITION  long-chain-fatty-acid--CoA ligase 1 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505587
VERSION     XP_077505587.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649461.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..682
                     /product="long-chain-fatty-acid--CoA ligase 1 isoform X3"
                     /calculated_mol_wt=75669
     Region          103..679
                     /region_name="LC-FACS_euk"
                     /note="Eukaryotic long-chain fatty acid CoA synthetase
                     (LC-FACS); cd05927"
                     /db_xref="CDD:341250"
     Site            order(258,261..266,268..269)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341250"
     Site            order(261,378..379,441..446,525,537,540,552,658)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341250"
     Site            order(261,304..305,350,352..353,356,378..379,441..446,525,
                     537,540,549..552,635)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341250"
     Site            order(304,352..353,356,378,549..551,633,635)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341250"
     CDS             1..682
                     /gene="LOC144115194"
                     /coded_by="XM_077649461.1:245..2293"
                     /db_xref="GeneID:144115194"
ORIGIN      
        1 mseylsyvgg vhglaaaaga aaalgltymf grsantiept vsldnqsrll tglerirtsr
       61 lcpdgnhiey lyedcrtlfd vmyrgarvsn ngpcmgwrvp gsgeykwihy nqvlerikci
      121 acglvklgek pgqqtfigly tqncvewivt eqacyyfsmi ivplydtlga aacafiinqa
      181 dietvvcdtq ekvecllrqa getpklkrii viknvsedvk ekarnaniml mkfseleelg
      241 eknfrhplpp httdlatvcy tsgttgnpkg vmlthlniis dlsavmrqlg dvaptgddvm
      301 lsflplahml erccemaiym iggkvgffsg dikelgedmk alrptisptv prllnrihdk
      361 visascgsff krtllnlalk skeaelqnfi vrrnglwdrl vfksvhesmg grlrlmvvgs
      421 aplapsvitf vrcalgcviv egygqtecta pctltfpgdy naghvgppig acnvklvdvp
      481 dmeyyasnne geicvkgptv fqgylkdpek taevldkdgw lhtgdigkwl dngclkvidr
      541 kkhifklsqg eyiapeklen ifirslfvaq ifvhgeslks clvavvipdq eylmqwaren
      601 sisgtfqelc rnnivkksil ddlsrlgkla glksfellkd iylhsdvlsv dnglltptlk
      661 tkrpecrkyf lsqienmyrs la
//
LOCUS       XP_077505588             682 aa            linear   INV 25-NOV-2025
DEFINITION  long-chain-fatty-acid--CoA ligase 1 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505588
VERSION     XP_077505588.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649462.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..682
                     /product="long-chain-fatty-acid--CoA ligase 1 isoform X3"
                     /calculated_mol_wt=75669
     Region          103..679
                     /region_name="LC-FACS_euk"
                     /note="Eukaryotic long-chain fatty acid CoA synthetase
                     (LC-FACS); cd05927"
                     /db_xref="CDD:341250"
     Site            order(258,261..266,268..269)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341250"
     Site            order(261,378..379,441..446,525,537,540,552,658)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341250"
     Site            order(261,304..305,350,352..353,356,378..379,441..446,525,
                     537,540,549..552,635)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341250"
     Site            order(304,352..353,356,378,549..551,633,635)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341250"
     CDS             1..682
                     /gene="LOC144115194"
                     /coded_by="XM_077649462.1:166..2214"
                     /db_xref="GeneID:144115194"
ORIGIN      
        1 mseylsyvgg vhglaaaaga aaalgltymf grsantiept vsldnqsrll tglerirtsr
       61 lcpdgnhiey lyedcrtlfd vmyrgarvsn ngpcmgwrvp gsgeykwihy nqvlerikci
      121 acglvklgek pgqqtfigly tqncvewivt eqacyyfsmi ivplydtlga aacafiinqa
      181 dietvvcdtq ekvecllrqa getpklkrii viknvsedvk ekarnaniml mkfseleelg
      241 eknfrhplpp httdlatvcy tsgttgnpkg vmlthlniis dlsavmrqlg dvaptgddvm
      301 lsflplahml erccemaiym iggkvgffsg dikelgedmk alrptisptv prllnrihdk
      361 visascgsff krtllnlalk skeaelqnfi vrrnglwdrl vfksvhesmg grlrlmvvgs
      421 aplapsvitf vrcalgcviv egygqtecta pctltfpgdy naghvgppig acnvklvdvp
      481 dmeyyasnne geicvkgptv fqgylkdpek taevldkdgw lhtgdigkwl dngclkvidr
      541 kkhifklsqg eyiapeklen ifirslfvaq ifvhgeslks clvavvipdq eylmqwaren
      601 sisgtfqelc rnnivkksil ddlsrlgkla glksfellkd iylhsdvlsv dnglltptlk
      661 tkrpecrkyf lsqienmyrs la
//
LOCUS       XP_077505589             682 aa            linear   INV 25-NOV-2025
DEFINITION  long-chain-fatty-acid--CoA ligase 1 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505589
VERSION     XP_077505589.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649463.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..682
                     /product="long-chain-fatty-acid--CoA ligase 1 isoform X3"
                     /calculated_mol_wt=75669
     Region          103..679
                     /region_name="LC-FACS_euk"
                     /note="Eukaryotic long-chain fatty acid CoA synthetase
                     (LC-FACS); cd05927"
                     /db_xref="CDD:341250"
     Site            order(258,261..266,268..269)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341250"
     Site            order(261,378..379,441..446,525,537,540,552,658)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341250"
     Site            order(261,304..305,350,352..353,356,378..379,441..446,525,
                     537,540,549..552,635)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341250"
     Site            order(304,352..353,356,378,549..551,633,635)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341250"
     CDS             1..682
                     /gene="LOC144115194"
                     /coded_by="XM_077649463.1:141..2189"
                     /db_xref="GeneID:144115194"
ORIGIN      
        1 mseylsyvgg vhglaaaaga aaalgltymf grsantiept vsldnqsrll tglerirtsr
       61 lcpdgnhiey lyedcrtlfd vmyrgarvsn ngpcmgwrvp gsgeykwihy nqvlerikci
      121 acglvklgek pgqqtfigly tqncvewivt eqacyyfsmi ivplydtlga aacafiinqa
      181 dietvvcdtq ekvecllrqa getpklkrii viknvsedvk ekarnaniml mkfseleelg
      241 eknfrhplpp httdlatvcy tsgttgnpkg vmlthlniis dlsavmrqlg dvaptgddvm
      301 lsflplahml erccemaiym iggkvgffsg dikelgedmk alrptisptv prllnrihdk
      361 visascgsff krtllnlalk skeaelqnfi vrrnglwdrl vfksvhesmg grlrlmvvgs
      421 aplapsvitf vrcalgcviv egygqtecta pctltfpgdy naghvgppig acnvklvdvp
      481 dmeyyasnne geicvkgptv fqgylkdpek taevldkdgw lhtgdigkwl dngclkvidr
      541 kkhifklsqg eyiapeklen ifirslfvaq ifvhgeslks clvavvipdq eylmqwaren
      601 sisgtfqelc rnnivkksil ddlsrlgkla glksfellkd iylhsdvlsv dnglltptlk
      661 tkrpecrkyf lsqienmyrs la
//
LOCUS       XP_077505590            1276 aa            linear   INV 25-NOV-2025
DEFINITION  COPII coat complex component secretory 31 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505590
VERSION     XP_077505590.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649464.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1276
                     /product="COPII coat complex component secretory 31
                     isoform X1"
                     /calculated_mol_wt=136244
     Region          11..65
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          70..116
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          88..339
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Site            order(90,94,101..102,118,120,138,143,149..150,161..162,
                     183,188,194..195,208,229,234,242..243,256..257,276,280,
                     286..287,303..304,322,327,333..334)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          124..161
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          171..206
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          212..255
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          261..302
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          308..333
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          568..>696
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cl14807"
                     /db_xref="CDD:449359"
     Site            order(575..576,577..578,580,582,592,594,596..597,599..600,
                     604,610,617,625,630,633,658..659)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Region          <873..>1100
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     CDS             1..1276
                     /gene="Sec31"
                     /coded_by="XM_077649464.1:81..3911"
                     /db_xref="GeneID:144115196"
ORIGIN      
        1 mkvkeidrta nvawspavhh piyiaagtaa qqldstfstt aaleiyalnl tepgldmglv
       61 gsldsdhrfh kiawgchgis dgempsgvvv ggadagnllv ydpaklikge dalicqknkh
      121 tgpvyaldfn tfqanllssg stdseifiwd lnspnapmtp gaksqphedi sclawnrqvq
      181 hilastfpar civwdlrkne piikvsdtta rvhckavawh pevatqlcla seddhapvvq
      241 lwdlrfatsp lktlehhqkg vlsiawcpqd pdlllscgkd nrilcwnpns nvpggevvce
      301 iptgnqwnfd vawcprnpav issasfdghv giysllggqq qaqassrlae sfpgadtlaq
      361 lppayqqqqq qqlqqrggti sllkppkwlr kpvgasfgfg gklisftkqq hqqqqqssgg
      421 aplaqlihis qvvtehtlma qsaeleaals tgafadfcqr kaqdchedpn lrlawsfiqa
      481 nfetsprakm lsllgydpdd vtsqlsvtgq esedtddqdl sndigsvsls atvngelled
      541 sssafdsiaa khkekeftpf tistnsgdtd gllnkalltg nveaavnlcl qegrwaeali
      601 laqaggvall qrtqcrffkq atsdsaklis avvhsnwahv vanceiscwk ealaavltya
      661 kpedfavlce slgsrlenek ntallpyaml cyicagnlek latcwvrvqs rsnsqqslqe
      721 lveqvmmlra algqlgsqpq piasgvlasl lshyagllaa qgslrsainy lvdttapsla
      781 ilrdricqhl gmpsphfpfp rvdvrgqfsp paaaaagnvq hgghltmgss rpglsslgag
      841 grftgstapa srarmastvs ssqsfyqggq qvppagapvn qfahrdsgsy agfgasvsqh
      901 sepvfqppva ptppppvdpv tssahskgpr srypsvhdpc vyneqqqgyg qayyqppsql
      961 pypssqaqsy mpqpysqsqs apgyytedqg fgdqgstytl ansttpqytn vygpsavssn
     1021 ttiqtaqlap plpaassiaa vpsssslgsa pppgwndppp vkpsaklsqg tpvqtfdlas
     1081 pittplvgap apavpetpap tfqggyvppp mpqfasqplp satqvlpqqd dpphfsilnp
     1141 lttaaaspsp aapppppppk ekgpipkenq vlqdvfeelr lncaqtatnp qikrklddva
     1201 rklenlydrl rdnaiapttt hglhqivqai qqsdyntals vhsylvsssn fsetssflpg
     1261 lkallqvaqq lgvclq
//
LOCUS       XP_077505591            1275 aa            linear   INV 25-NOV-2025
DEFINITION  COPII coat complex component secretory 31 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505591
VERSION     XP_077505591.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649465.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1275
                     /product="COPII coat complex component secretory 31
                     isoform X2"
                     /calculated_mol_wt=136145
     Region          11..65
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          70..116
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          88..339
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Site            order(90,94,101..102,118,120,138,143,149..150,161..162,
                     183,188,194..195,208,229,234,242..243,256..257,276,280,
                     286..287,303..304,322,327,333..334)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          124..161
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          171..206
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          212..255
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          261..302
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          308..333
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          567..>695
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cl14807"
                     /db_xref="CDD:449359"
     Site            order(574..575,576..577,579,581,591,593,595..596,598..599,
                     603,609,616,624,629,632,657..658)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Region          <872..>1099
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     CDS             1..1275
                     /gene="Sec31"
                     /coded_by="XM_077649465.1:81..3908"
                     /db_xref="GeneID:144115196"
ORIGIN      
        1 mkvkeidrta nvawspavhh piyiaagtaa qqldstfstt aaleiyalnl tepgldmglv
       61 gsldsdhrfh kiawgchgis dgempsgvvv ggadagnllv ydpaklikge dalicqknkh
      121 tgpvyaldfn tfqanllssg stdseifiwd lnspnapmtp gaksqphedi sclawnrqvq
      181 hilastfpar civwdlrkne piikvsdtta rvhckavawh pevatqlcla seddhapvvq
      241 lwdlrfatsp lktlehhqkg vlsiawcpqd pdlllscgkd nrilcwnpns nvpggevvce
      301 iptgnqwnfd vawcprnpav issasfdghv giysllggqq qaqassrlae sfpgadtlaq
      361 lppayqqqqq qqlqqrggti sllkppkwlr kpvgasfgfg gklisftkqq hqqqqqssgg
      421 aplaqlihis qvvtehtlma qsaeleaals tgafadfcqr kaqdchedpn lrlawsfiqa
      481 nfetsprakm lsllgydpdd vtsqlsvtgq esedtddqdl sndigsvsls atngelleds
      541 ssafdsiaak hkekeftpft istnsgdtdg llnkalltgn veaavnlclq egrwaealil
      601 aqaggvallq rtqcrffkqa tsdsaklisa vvhsnwahvv anceiscwke alaavltyak
      661 pedfavlces lgsrlenekn tallpyamlc yicagnlekl atcwvrvqsr snsqqslqel
      721 veqvmmlraa lgqlgsqpqp iasgvlasll shyagllaaq gslrsainyl vdttapslai
      781 lrdricqhlg mpsphfpfpr vdvrgqfspp aaaaagnvqh gghltmgssr pglsslgagg
      841 rftgstapas rarmastvss sqsfyqggqq vppagapvnq fahrdsgsya gfgasvsqhs
      901 epvfqppvap tppppvdpvt ssahskgprs rypsvhdpcv yneqqqgygq ayyqppsqlp
      961 ypssqaqsym pqpysqsqsa pgyytedqgf gdqgstytla nsttpqytnv ygpsavssnt
     1021 tiqtaqlapp lpaassiaav psssslgsap ppgwndpppv kpsaklsqgt pvqtfdlasp
     1081 ittplvgapa pavpetpapt fqggyvpppm pqfasqplps atqvlpqqdd pphfsilnpl
     1141 ttaaaspspa apppppppke kgpipkenqv lqdvfeelrl ncaqtatnpq ikrklddvar
     1201 klenlydrlr dnaiapttth glhqivqaiq qsdyntalsv hsylvsssnf setssflpgl
     1261 kallqvaqql gvclq
//
LOCUS       XP_077505592            1262 aa            linear   INV 25-NOV-2025
DEFINITION  COPII coat complex component secretory 31 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505592
VERSION     XP_077505592.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649466.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1262
                     /product="COPII coat complex component secretory 31
                     isoform X3"
                     /calculated_mol_wt=134695
     Region          11..65
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          70..116
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          88..339
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Site            order(90,94,101..102,118,120,138,143,149..150,161..162,
                     183,188,194..195,208,229,234,242..243,256..257,276,280,
                     286..287,303..304,322,327,333..334)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          124..161
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          171..206
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          212..255
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          261..302
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          308..333
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          568..>696
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cl14807"
                     /db_xref="CDD:449359"
     Site            order(575..576,577..578,580,582,592,594,596..597,599..600,
                     604,610,617,625,630,633,658..659)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Region          <873..>1100
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     CDS             1..1262
                     /gene="Sec31"
                     /coded_by="XM_077649466.1:81..3869"
                     /db_xref="GeneID:144115196"
ORIGIN      
        1 mkvkeidrta nvawspavhh piyiaagtaa qqldstfstt aaleiyalnl tepgldmglv
       61 gsldsdhrfh kiawgchgis dgempsgvvv ggadagnllv ydpaklikge dalicqknkh
      121 tgpvyaldfn tfqanllssg stdseifiwd lnspnapmtp gaksqphedi sclawnrqvq
      181 hilastfpar civwdlrkne piikvsdtta rvhckavawh pevatqlcla seddhapvvq
      241 lwdlrfatsp lktlehhqkg vlsiawcpqd pdlllscgkd nrilcwnpns nvpggevvce
      301 iptgnqwnfd vawcprnpav issasfdghv giysllggqq qaqassrlae sfpgadtlaq
      361 lppayqqqqq qqlqqrggti sllkppkwlr kpvgasfgfg gklisftkqq hqqqqqssgg
      421 aplaqlihis qvvtehtlma qsaeleaals tgafadfcqr kaqdchedpn lrlawsfiqa
      481 nfetsprakm lsllgydpdd vtsqlsvtgq esedtddqdl sndigsvsls atvngelled
      541 sssafdsiaa khkekeftpf tistnsgdtd gllnkalltg nveaavnlcl qegrwaeali
      601 laqaggvall qrtqcrffkq atsdsaklis avvhsnwahv vanceiscwk ealaavltya
      661 kpedfavlce slgsrlenek ntallpyaml cyicagnlek latcwvrvqs rsnsqqslqe
      721 lveqvmmlra algqlgsqpq piasgvlasl lshyagllaa qgslrsainy lvdttapsla
      781 ilrdricqhl gmpsphfpfp rvdvrgqfsp paaaaagnvq hgghltmgss rpglsslgag
      841 grftgstapa srarmastvs ssqsfyqggq qvppagapvn qfahrdsgsy agfgasvsqh
      901 sepvfqppva ptppppvdpv tssahskgpr srypsvhdpc vyneqqqgyg qayyqppsql
      961 pypssqaqsy mpqpysqsqs apgyytedqg fgdqgstytl ansttpqytn vygpsavssn
     1021 ttiqtaqlap plpaassiaa vpsssslgsa pppgwndppp vkpsaklsqg tpvqtfdlas
     1081 pittplvgap apavpetpap tfqggyvppp mpqfasqplp satqvlpqqa aaspspaapp
     1141 pppppkekgp ipkenqvlqd vfeelrlnca qtatnpqikr klddvarkle nlydrlrdna
     1201 iapttthglh qivqaiqqsd yntalsvhsy lvsssnfset ssflpglkal lqvaqqlgvc
     1261 lq
//
LOCUS       XP_077505593            1261 aa            linear   INV 25-NOV-2025
DEFINITION  COPII coat complex component secretory 31 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505593
VERSION     XP_077505593.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649467.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1261
                     /product="COPII coat complex component secretory 31
                     isoform X4"
                     /calculated_mol_wt=134596
     Region          11..65
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          70..116
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          88..339
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Site            order(90,94,101..102,118,120,138,143,149..150,161..162,
                     183,188,194..195,208,229,234,242..243,256..257,276,280,
                     286..287,303..304,322,327,333..334)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          124..161
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          171..206
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          212..255
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          261..302
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          308..333
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          567..>695
                     /region_name="ACE1-Sec16-like"
                     /note="Ancestral coatomer element 1 (ACE1) of COPII coat
                     complex assembly protein Sec16; cl14807"
                     /db_xref="CDD:449359"
     Site            order(574..575,576..577,579,581,591,593,595..596,598..599,
                     603,609,616,624,629,632,657..658)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187750"
     Region          <872..>1099
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     CDS             1..1261
                     /gene="Sec31"
                     /coded_by="XM_077649467.1:81..3866"
                     /db_xref="GeneID:144115196"
ORIGIN      
        1 mkvkeidrta nvawspavhh piyiaagtaa qqldstfstt aaleiyalnl tepgldmglv
       61 gsldsdhrfh kiawgchgis dgempsgvvv ggadagnllv ydpaklikge dalicqknkh
      121 tgpvyaldfn tfqanllssg stdseifiwd lnspnapmtp gaksqphedi sclawnrqvq
      181 hilastfpar civwdlrkne piikvsdtta rvhckavawh pevatqlcla seddhapvvq
      241 lwdlrfatsp lktlehhqkg vlsiawcpqd pdlllscgkd nrilcwnpns nvpggevvce
      301 iptgnqwnfd vawcprnpav issasfdghv giysllggqq qaqassrlae sfpgadtlaq
      361 lppayqqqqq qqlqqrggti sllkppkwlr kpvgasfgfg gklisftkqq hqqqqqssgg
      421 aplaqlihis qvvtehtlma qsaeleaals tgafadfcqr kaqdchedpn lrlawsfiqa
      481 nfetsprakm lsllgydpdd vtsqlsvtgq esedtddqdl sndigsvsls atngelleds
      541 ssafdsiaak hkekeftpft istnsgdtdg llnkalltgn veaavnlclq egrwaealil
      601 aqaggvallq rtqcrffkqa tsdsaklisa vvhsnwahvv anceiscwke alaavltyak
      661 pedfavlces lgsrlenekn tallpyamlc yicagnlekl atcwvrvqsr snsqqslqel
      721 veqvmmlraa lgqlgsqpqp iasgvlasll shyagllaaq gslrsainyl vdttapslai
      781 lrdricqhlg mpsphfpfpr vdvrgqfspp aaaaagnvqh gghltmgssr pglsslgagg
      841 rftgstapas rarmastvss sqsfyqggqq vppagapvnq fahrdsgsya gfgasvsqhs
      901 epvfqppvap tppppvdpvt ssahskgprs rypsvhdpcv yneqqqgygq ayyqppsqlp
      961 ypssqaqsym pqpysqsqsa pgyytedqgf gdqgstytla nsttpqytnv ygpsavssnt
     1021 tiqtaqlapp lpaassiaav psssslgsap ppgwndpppv kpsaklsqgt pvqtfdlasp
     1081 ittplvgapa pavpetpapt fqggyvpppm pqfasqplps atqvlpqqaa aspspaappp
     1141 ppppkekgpi pkenqvlqdv feelrlncaq tatnpqikrk lddvarklen lydrlrdnai
     1201 apttthglhq ivqaiqqsdy ntalsvhsyl vsssnfsets sflpglkall qvaqqlgvcl
     1261 q
//
LOCUS       XP_077505594             152 aa            linear   INV 25-NOV-2025
DEFINITION  UPAR/Ly6 domain-containing protein crok-like [Amblyomma
            americanum].
ACCESSION   XP_077505594
VERSION     XP_077505594.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649468.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..152
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..152
                     /product="UPAR/Ly6 domain-containing protein crok-like"
                     /calculated_mol_wt=16415
     Region          23..125
                     /region_name="TFP_LU_ECD_Crok"
                     /note="extracellular domain (ECD), Ly6/uPAR superfamily,
                     three finger domain (TFD) fold, found in Drosophila
                     melanogaster protein crooked and similar proteins;
                     cd23592"
                     /db_xref="CDD:467121"
     CDS             1..152
                     /gene="LOC144115198"
                     /coded_by="XM_077649468.1:322..780"
                     /db_xref="GeneID:144115198"
ORIGIN      
        1 metgavvtal tlclallwgs geaikcwecn shadprcadp fdntslpiae caarslqhyp
       61 glaatmcrki rqkvngnwrf irscaflgep gegtydenyc lmrtgtydvf vetctcnskd
      121 gcntaphhgg plwllalata vatwtllgal rq
//
LOCUS       XP_077505596             156 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115199 [Amblyomma americanum].
ACCESSION   XP_077505596
VERSION     XP_077505596.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649470.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..156
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..156
                     /product="uncharacterized protein LOC144115199"
                     /calculated_mol_wt=17724
     Region          26..129
                     /region_name="QVR"
                     /note="Sleepless protein; pfam17064"
                     /db_xref="CDD:435716"
     CDS             1..156
                     /gene="LOC144115199"
                     /coded_by="XM_077649470.1:130..600"
                     /db_xref="GeneID:144115199"
ORIGIN      
        1 mksleprsvl trtcvflacf yqvlaikcyq csssedpkge dhcgayahyd ktkntavecl
       61 geeakalgtf cykrimqgpr gfiwdgrwrs verrcaqise rgvnwgcdwg yhkngvywee
      121 cycaedscng adqlrvlhal lvlcgsltll rhldff
//
LOCUS       XP_077505597             617 aa            linear   INV 25-NOV-2025
DEFINITION  syntaxin-binding protein Rop isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505597
VERSION     XP_077505597.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649471.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..617
                     /product="syntaxin-binding protein Rop isoform X1"
                     /calculated_mol_wt=70499
     Region          26..611
                     /region_name="Sec1"
                     /note="Sec1 family; pfam00995"
                     /db_xref="CDD:460020"
     CDS             1..617
                     /gene="Rop"
                     /coded_by="XM_077649471.1:85..1938"
                     /db_xref="GeneID:144115200"
ORIGIN      
        1 malksvvglk itndviravk kkgewkvlvv dqlgmrmisa cckmhelase gitivedlnk
       61 kreplpniea vyliapteks vralmsdfpt parhmyrcah ifftekcpdd lftdickspl
      121 akviktlkev niaflpyesq vfsldspetf qfyynpnrig ertsnleria eqvatlcatl
      181 geypslryrs dfdhnvdlaq mlyqrldayk adeptmgegp ekmrsqliil drgfdcvspl
      241 lheltfqama ydllpiendv ykfestsgnd vrekevllde kddlwielrh qhiavvsqav
      301 tkqlkkfies krmtssgdks slkdlttmik kmpqyqkeln kystqlhlae dcmkcyqgyv
      361 drlckveqdl amgtdaegek ikdpmrnivp illdtsvsnf dkiriillyi lskngiseen
      421 ltkliqhaqi patekciitn mahigvnivt dqreleklpp fnshtpllls qqftnevlgl
      481 gsrkkvyhvp rkeriteqty qmsrwtpvlk dimedaiedk ldvkhfpyls ggrvpptgyg
      541 rsaptsqryg hwhkdkntpn vknvprlivf viggmtysem rcayevtkev knweviigsd
      601 hiltpegfls dlrdlst
//
LOCUS       XP_077505598             588 aa            linear   INV 25-NOV-2025
DEFINITION  syntaxin-binding protein Rop isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505598
VERSION     XP_077505598.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649472.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..588
                     /product="syntaxin-binding protein Rop isoform X2"
                     /calculated_mol_wt=67167
     Region          26..582
                     /region_name="Sec1"
                     /note="Sec1 family; pfam00995"
                     /db_xref="CDD:460020"
     CDS             1..588
                     /gene="Rop"
                     /coded_by="XM_077649472.1:85..1851"
                     /db_xref="GeneID:144115200"
ORIGIN      
        1 malksvvglk itndviravk kkgewkvlvv dqlgmrmisa cckmhelase gitivedlnk
       61 kreplpniea vyliapteks vralmsdfpt parhmyrcah ifftekcpdd lftdickspl
      121 akviktlkev niaflpyesq vfsldspetf qfyynpnrig ertsnleria eqvatlcatl
      181 geypslryrs dfdhnvdlaq mlyqrldayk adeptmgegp ekmrsqliil drgfdcvspl
      241 lheltfqama ydllpiendv ykfestsgnd vrekevllde kddlwielrh qhiavvsqav
      301 tkqlkkfies krmtssgdks slkdlttmik kmpqyqkeln kystqlhlae dcmkcyqgyv
      361 drlckveqdl amgtdaegek ikdpmrnivp illdtsvsnf dkiriillyi lskngiseen
      421 ltkliqhaqi patekciitn mahigvnivt dgsrkkvyhv prkeriteqt yqmsrwtpvl
      481 kdimedaied kldvkhfpyl sggrvpptgy grsaptsqry ghwhkdkntp nvknvprliv
      541 fviggmtyse mrcayevtke vknweviigs dhiltpegfl sdlrdlst
//
LOCUS       XP_077505599             178 aa            linear   INV 25-NOV-2025
DEFINITION  ribosomal protein L28 [Amblyomma americanum].
ACCESSION   XP_077505599
VERSION     XP_077505599.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649473.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..178
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..178
                     /product="ribosomal protein L28"
                     /calculated_mol_wt=20170
     Region          42..160
                     /region_name="Ribosomal_L28e"
                     /note="Ribosomal L28e protein family; pfam01778"
                     /db_xref="CDD:460323"
     CDS             1..178
                     /gene="RpL28"
                     /coded_by="XM_077649473.1:50..586"
                     /db_xref="GeneID:144115201"
ORIGIN      
        1 mtsltnrssr agtrikvaas sgattrvipf cprawvtmsa elqwmivknr ssflvkkrmi
       61 ktwfsidpln pkgthakrys givrdkalti ephvsgkgvt lvykkqayrk kpaklldrvd
      121 lmngarrtmt sikkwvkghk yyrkdlqtlc mrrasailrs qkaivpkkkt prptkkad
//
LOCUS       XP_077505600             669 aa            linear   INV 25-NOV-2025
DEFINITION  eEF1A lysine and N-terminal methyltransferase-like [Amblyomma
            americanum].
ACCESSION   XP_077505600
VERSION     XP_077505600.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649474.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..669
                     /product="eEF1A lysine and N-terminal
                     methyltransferase-like"
                     /calculated_mol_wt=75336
     Region          29..172
                     /region_name="UbiE"
                     /note="Ubiquinone/menaquinone biosynthesis C-methylase
                     UbiE/MenG [Coenzyme transport and metabolism]; COG2226"
                     /db_xref="CDD:441828"
     Region          454..615
                     /region_name="SpeE"
                     /note="Spermidine synthase (polyamine
                     aminopropyltransferase) [Amino acid transport and
                     metabolism]; COG0421"
                     /db_xref="CDD:440190"
     CDS             1..669
                     /gene="LOC144115202"
                     /coded_by="XM_077649474.1:124..2133"
                     /db_xref="GeneID:144115202"
ORIGIN      
        1 mnllpktsse fasekywnef frkrgkaafe wygeywqlag ticsylkktd kllivgcgns
       61 slsadlydsg ytnnvsidis dvvirqmkhk yaktrphiqf qqmdaaemqy kdeefsvvld
      121 kgtvdaltpn sdettiakls avlaevsrvl rvggrfvcis llqthvleal lkwfcsdpsw
      181 twvirvhrcv daekvdegts rlvlpvfvvv ftklkrlpgl etvtelaldp dlkprrvpvs
      241 tvcsevsslq qyaflrhhma rrrlesndev mlelcaagsg vpryrlfvcd rpptssapik
      301 faiflapqgr esewlfgcme grrqlavscq aerlvvvhlc rdqtysgmee vknevsqnim
      361 elappsyvlg mqvpflsagk dvghrevrhr gksaisgeyv iedvtmedgg gvrrlifldk
      421 phviqteakl kqvkckgkkk akvwqvvpen llceyykymv agiafimpkg tegpasalmv
      481 glgggtlplf lftkfpelrl svieldpevv diarrwylpe hcpmdirved glntferlsk
      541 egktydlvfl dvdskdlseg ltcppmeflt ehklrevaav teytgavvmn fvcrnetlkk
      601 evyqrlngif dtvlvqkipd nvnevvyltn dksdgmesrf isnvqrlski lkgvdnadis
      661 almnglkva
//
LOCUS       XP_077505601             895 aa            linear   INV 25-NOV-2025
DEFINITION  vacuolar protein sorting 39 [Amblyomma americanum].
ACCESSION   XP_077505601
VERSION     XP_077505601.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649475.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..895
                     /product="vacuolar protein sorting 39"
                     /calculated_mol_wt=101382
     Region          24..281
                     /region_name="CNH"
                     /note="CNH domain; cl02434"
                     /db_xref="CDD:470577"
     Region          458..560
                     /region_name="Vps39_1"
                     /note="Vacuolar sorting protein 39 domain 1; pfam10366"
                     /db_xref="CDD:371008"
     Region          587..739
                     /region_name="Clathrin"
                     /note="Region in Clathrin and VPS; pfam00637"
                     /db_xref="CDD:459884"
     Region          780..887
                     /region_name="Vps39_2"
                     /note="Vacuolar sorting protein 39 domain 2; pfam10367"
                     /db_xref="CDD:402126"
     CDS             1..895
                     /gene="Vps39"
                     /coded_by="XM_077649475.1:136..2823"
                     /db_xref="GeneID:144115203"
ORIGIN      
        1 mydaytaipi lkklplkies vaahgdklyl gtkeghllvy svvekdsadg pkfavnlkcs
       61 nksfakkpit qlcvipelcl lislsdgsvs vhnlslepnt ppiecpalak ckgctlfavn
      121 vqetetltge vkitlmlcaa vrrklelfyw knntfcehpq glcvpdtprs ivwcgdesll
      181 vgfrseyyil klsgetkqlf ptgkqpeplc vklqensfal grddmtifvn regqpthkya
      241 vnwseapisl cydypylisa qsfgvevrtv eprlliqkvn llkpklivpc kegqlfvvap
      301 ggaiwcfvrt rvqdqipqvl kdkcfelalk ladlsdqsde ernaskrhiq nlhafdlfck
      361 kkfeesltif mdletdpshv iglfpdllpe dyrnsisypd qapdlrdadl eaglyalvdy
      421 lvhvrcklls dsqhepaltg ivqgsktvks kkqllqiidt tmlkcyletn valvasllrl
      481 pdnfchldec eralkkhqkl seliilyqqk nqhekaldll mkeahkadsp lkghertvgy
      541 lqhlgrkhme lilryslwvl eehpeeglki fvedqqekeg ealprdvvle flskkapqlv
      601 ipylehvihk wkdqtemfhn tlihkyiesv rsllaqqhsa vdpgepglvg klrkdlvhfl
      661 essdrytaen fpthllsdgl feeaavvmgk lgrhsealev yiyvlkdplk aehycasqys
      721 rnpernrdvf lillqmylqp pdegsrvldl crrtaasiag lpypltvmeg creqnlrgal
      781 kilmdhvkei dplralqmlp advlvsdvrd flrevldkgt kelhaaglqr sllfaehlqv
      841 kercirvksl kitlteldac cvcqkrigrs afarypdgai vhyscrdgye lrlas
//
LOCUS       XP_077505602             540 aa            linear   INV 25-NOV-2025
DEFINITION  annexin-B12-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505602
VERSION     XP_077505602.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649476.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..540
                     /product="annexin-B12-like isoform X1"
                     /calculated_mol_wt=57847
     Region          32..>182
                     /region_name="dnaA"
                     /note="chromosomal replication initiator protein DnaA;
                     cl42516"
                     /db_xref="CDD:455861"
     Region          241..305
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          314..377
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          396..461
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          471..536
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     CDS             1..540
                     /gene="LOC144115204"
                     /coded_by="XM_077649476.1:200..1822"
                     /db_xref="GeneID:144115204"
ORIGIN      
        1 msypggsgsp yppqpgypgy psqdrtvypm gavyaappvt qpapgyppqm pnqaypqsss
       61 pgfpptqpgf apqpsypsqp gyptqpgypt qpgyptqpgy psqpgcpsqp gypsqpsyps
      121 qpgypqpaat gypsstqgyp gyptgqssyp apvsaasypa tqaapspyps agypsagsag
      181 htggygasyg aaaygaapsy ssaaqvthsa sytgqrlrds lgnarsmati rpypgfnpqd
      241 dsqalrkamk gfgtdeaaii ailakrtsdq rqvitttykq lfgrdlvkdl kselsgkfed
      301 vivglmtply eflaselkaa mkgagtdedc lieilctrtn aeiaaikqiy kqkydkdlek
      361 avisetsgdf qrilvsmlta srqegvpvda nraaedaqkl yqagvakwgt destfnaila
      421 sqsydqlrqv freyvrfanh dimeaikkem sgnfrqallt ivksvyntel yfaeklhdam
      481 kgagtddktl irivvsrcet dlaivkqeyq raygksleda ikgdtsgdyr kvllalvsgn
//
LOCUS       XP_077505603             314 aa            linear   INV 25-NOV-2025
DEFINITION  annexin-B12-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505603
VERSION     XP_077505603.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649477.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..314
                     /product="annexin-B12-like isoform X2"
                     /calculated_mol_wt=34904
     Region          15..79
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          88..151
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          170..235
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          245..310
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     CDS             1..314
                     /gene="LOC144115204"
                     /coded_by="XM_077649477.1:452..1396"
                     /db_xref="GeneID:144115204"
ORIGIN      
        1 matirpypgf npqddsqalr kamkgfgtde aaiiailakr tsdqrqvitt tykqlfgrdl
       61 vkdlkselsg kfedvivglm tplyeflase lkaamkgagt dedclieilc trtnaeiaai
      121 kqiykqkydk dlekaviset sgdfqrilvs mltasrqegv pvdanraaed aqklyqagva
      181 kwgtdestfn ailasqsydq lrqvfreyvr fanhdimeai kkemsgnfrq alltivksvy
      241 ntelyfaekl hdamkgagtd dktlirivvs rcetdlaivk qeyqraygks ledaikgdts
      301 gdyrkvllal vsgn
//
LOCUS       XP_077505604             118 aa            linear   INV 25-NOV-2025
DEFINITION  biogenesis of lysosome-related organelles complex 1 subunit 2
            [Amblyomma americanum].
ACCESSION   XP_077505604
VERSION     XP_077505604.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649478.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..118
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..118
                     /product="biogenesis of lysosome-related organelles
                     complex 1 subunit 2"
                     /calculated_mol_wt=13440
     Region          20..113
                     /region_name="BLOC1_2"
                     /note="Biogenesis of lysosome-related organelles complex-1
                     subunit 2; pfam10046"
                     /db_xref="CDD:462951"
     CDS             1..118
                     /gene="Blos2"
                     /coded_by="XM_077649478.1:147..503"
                     /db_xref="GeneID:144115206"
ORIGIN      
        1 mesgdeates pklnelaatm fektgeyiqa elsstlddyr lteemnklti tkysdmkqia
       61 gnvtkalkdl nekyrclkpy ldqidqveds vtkleqaayk ldayskrlea kfkalekr
//
LOCUS       XP_077505605             377 aa            linear   INV 25-NOV-2025
DEFINITION  annexin-B12-like [Amblyomma americanum].
ACCESSION   XP_077505605
VERSION     XP_077505605.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649479.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..377
                     /product="annexin-B12-like"
                     /calculated_mol_wt=42120
     Region          77..142
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          149..214
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          233..298
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          308..373
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     CDS             1..377
                     /gene="LOC144115207"
                     /coded_by="XM_077649479.1:288..1421"
                     /db_xref="GeneID:144115207"
ORIGIN      
        1 mygsrsgysg sgysrsdyag pvsgmpyppt tpntaparda ayyetspryy dgtvtgtscy
       61 apgkgtlkth mnfnvqqdve alkkamrgwt tdhaaiidil carsepqrqe ivttykqsfg
      121 rdlvedakss frgdlediil gllyplheyl arelrkaiag lgtdeeclve ilctqsnqdi
      181 rlikdhyqri fkkdlekdvi gdtsgafrrl lvsmcnaqre entpldpvra rndaaelhka
      241 gvqrrgtdvs afnriiatqs yeqlrlvfre ynavanhtvm eaiksemsgd lkngflaivk
      301 cvysppfyfa etlyhamkgl gtndrvlkrv vvsrcekdle likqeyldky kvslasaiqs
      361 dtsghyrkal laivqgn
//
LOCUS       XP_077505606             545 aa            linear   INV 25-NOV-2025
DEFINITION  annexin-B12-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505606
VERSION     XP_077505606.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649480.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..545
                     /product="annexin-B12-like isoform X1"
                     /calculated_mol_wt=59486
     Region          <62..214
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          245..310
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          317..382
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          401..466
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          476..541
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     CDS             1..545
                     /gene="LOC144115208"
                     /coded_by="XM_077649480.1:127..1764"
                     /db_xref="GeneID:144115208"
ORIGIN      
        1 msgssggasg gynpgcapgy ppgypptsss syptdavrpr gfvdpeeppr rmpsprpsfp
       61 ersatpavgn vssslepvsp etyytpqsgg tptdlsisat flsplpetyc ppsqssglpp
      121 egfsspisls pipgpfqhrt wgvaptplpr yssgvyppya tpplsaqsmy gsrsgysgsg
      181 ysrsdyagpv sgmpyppttp ntapardaay yetspryydg tvtgtscyap gkgtlkthmn
      241 fnvqqdveal kkamrgwttd haaiidilca rsepqrqeiv ttykqsfgrd lvedakssfr
      301 gdlediilgl lyplheylar elrkaiaglg tdeeclveil ctqsnqdirl ikdhyqrifk
      361 kdlekdvigd tsgvfrrllv smcnaqreen tpldpvrarn daaelhkagv qrwgtdvsaf
      421 nriiatqsye qlrlvfrayn avanhtvmea iksemsgdlk ngflaivkcv ysppfyfaet
      481 lyhamkglgt ndrvlkrvvv srcekdleli kqeyldkykv slasaiqsdt sghyrkalla
      541 ivqgn
//
LOCUS       XP_077505607             490 aa            linear   INV 25-NOV-2025
DEFINITION  annexin A13-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505607
VERSION     XP_077505607.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649481.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..490
                     /product="annexin A13-like isoform X2"
                     /calculated_mol_wt=53007
     Region          <62..214
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          245..310
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          317..382
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          401..466
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     CDS             1..490
                     /gene="LOC144115208"
                     /coded_by="XM_077649481.1:127..1599"
                     /db_xref="GeneID:144115208"
ORIGIN      
        1 msgssggasg gynpgcapgy ppgypptsss syptdavrpr gfvdpeeppr rmpsprpsfp
       61 ersatpavgn vssslepvsp etyytpqsgg tptdlsisat flsplpetyc ppsqssglpp
      121 egfsspisls pipgpfqhrt wgvaptplpr yssgvyppya tpplsaqsmy gsrsgysgsg
      181 ysrsdyagpv sgmpyppttp ntapardaay yetspryydg tvtgtscyap gkgtlkthmn
      241 fnvqqdveal kkamrgwttd haaiidilca rsepqrqeiv ttykqsfgrd lvedakssfr
      301 gdlediilgl lyplheylar elrkaiaglg tdeeclveil ctqsnqdirl ikdhyqrifk
      361 kdlekdvigd tsgvfrrllv smcnaqreen tpldpvrarn daaelhkagv qrwgtdvsaf
      421 nriiatqsye qlrlvfrayn avanhtvmea iksemsgdlk ngflaigprh qrqgaqasrg
      481 vamregpgad
//
LOCUS       XP_077505608             872 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115209 [Amblyomma americanum].
ACCESSION   XP_077505608
VERSION     XP_077505608.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649482.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..872
                     /product="uncharacterized protein LOC144115209"
                     /calculated_mol_wt=97871
     CDS             1..872
                     /gene="LOC144115209"
                     /coded_by="XM_077649482.1:69..2687"
                     /db_xref="GeneID:144115209"
ORIGIN      
        1 mdgdvtkkrr kryfykdeqf vvpkttlyrr qkevrlraqh casstsaand gdvngggvvg
       61 dllnlpsgeq paaeqaaqpa sptlsahdeq aggtsyddde dlfqtddfdr lgetpedsss
      121 tsgetenddg ereflasdfv eleaavipgs tttkaaaiim imafvvthgl twealndllr
      181 lidglfgfkg dvlprskfvf rklwssrker lvkrffycdt cgsvlvsvpg mssmrcpdce
      241 vstelcvlka rghffiildl keqmkcllak skaalferlv nlkaviqqgg aallqditsg
      301 smaeelrksg kigwmdltit intdgspvfk sssssvwpiq flinelppcd rlkncliggl
      361 wfgqhpymrt fltkfveein qfgritwkag htllssglhv lcccvdapar asvinmvqfn
      421 glfgcpwcyn caefhegaqr ymsvtvgeer tpgemskdme faekikdavn glkgqsplnh
      481 lkhfnivfgh tveymhcvli gvtkcltdqw fdsansqqpf yigrpvtmak vdrrllaikp
      541 phsftrlprs lkdkchwkas ewrhwllfya lpcclgilpq rylnhfallv eaiftlllee
      601 ltlqqinhag rlledfvsrm palygarlmt fnvhqllhla kaaknfgplw ahsafvfetg
      661 ngrvlraits angapdqvie rmimlqqvdl amalctvqeq arnfictmlg hpltlnatrv
      721 gnsymfgacd pfpvlsteek nslmlsfgyv psleehfrfs frstvlhstr yerakksnst
      781 aicttendff vinrifqvte netrecillc tkvvcmesqh klpphimecf rslsgvitvl
      841 klkdihapcv liefaeeegl yicklpnfie rd
//
LOCUS       XP_077505609             182 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115210 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505609
VERSION     XP_077505609.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649483.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..182
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..182
                     /product="uncharacterized protein LOC144115210 isoform X1"
                     /calculated_mol_wt=19857
     CDS             1..182
                     /gene="LOC144115210"
                     /coded_by="XM_077649483.1:475..1023"
                     /db_xref="GeneID:144115210"
ORIGIN      
        1 mktswrrghw mekkkeienl qleirslrkk lsqerqrnyd lqevvikgig elqehlkkrk
       61 tcqcseqqqv cnsdipatre pelgseslga rdvpgeglae laaaaptave aaassspspa
      121 qqglpvqaqi yvptssviaq erqgaprggd tqnggrenee rpsrctwksi paskrgrsac
      181 aa
//
LOCUS       XP_077505610             182 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115210 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505610
VERSION     XP_077505610.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649484.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..182
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..182
                     /product="uncharacterized protein LOC144115210 isoform X1"
                     /calculated_mol_wt=19857
     CDS             1..182
                     /gene="LOC144115210"
                     /coded_by="XM_077649484.1:472..1020"
                     /db_xref="GeneID:144115210"
ORIGIN      
        1 mktswrrghw mekkkeienl qleirslrkk lsqerqrnyd lqevvikgig elqehlkkrk
       61 tcqcseqqqv cnsdipatre pelgseslga rdvpgeglae laaaaptave aaassspspa
      121 qqglpvqaqi yvptssviaq erqgaprggd tqnggrenee rpsrctwksi paskrgrsac
      181 aa
//
LOCUS       XP_077505611             182 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115210 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505611
VERSION     XP_077505611.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649485.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..182
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..182
                     /product="uncharacterized protein LOC144115210 isoform X1"
                     /calculated_mol_wt=19857
     CDS             1..182
                     /gene="LOC144115210"
                     /coded_by="XM_077649485.1:144..692"
                     /db_xref="GeneID:144115210"
ORIGIN      
        1 mktswrrghw mekkkeienl qleirslrkk lsqerqrnyd lqevvikgig elqehlkkrk
       61 tcqcseqqqv cnsdipatre pelgseslga rdvpgeglae laaaaptave aaassspspa
      121 qqglpvqaqi yvptssviaq erqgaprggd tqnggrenee rpsrctwksi paskrgrsac
      181 aa
//
LOCUS       XP_077505612             182 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115210 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505612
VERSION     XP_077505612.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649486.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..182
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..182
                     /product="uncharacterized protein LOC144115210 isoform X1"
                     /calculated_mol_wt=19857
     CDS             1..182
                     /gene="LOC144115210"
                     /coded_by="XM_077649486.1:140..688"
                     /db_xref="GeneID:144115210"
ORIGIN      
        1 mktswrrghw mekkkeienl qleirslrkk lsqerqrnyd lqevvikgig elqehlkkrk
       61 tcqcseqqqv cnsdipatre pelgseslga rdvpgeglae laaaaptave aaassspspa
      121 qqglpvqaqi yvptssviaq erqgaprggd tqnggrenee rpsrctwksi paskrgrsac
      181 aa
//
LOCUS       XP_077505613             142 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115210 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505613
VERSION     XP_077505613.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649487.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..142
                     /product="uncharacterized protein LOC144115210 isoform X2"
                     /calculated_mol_wt=14854
     CDS             1..142
                     /gene="LOC144115210"
                     /coded_by="XM_077649487.1:442..870"
                     /db_xref="GeneID:144115210"
ORIGIN      
        1 mlskmlkrtg elqehlkkrk tcqcseqqqv cnsdipatre pelgseslga rdvpgeglae
       61 laaaaptave aaassspspa qqglpvqaqi yvptssviaq erqgaprggd tqnggrenee
      121 rpsrctwksi paskrgrsac aa
//
LOCUS       XP_077505614             545 aa            linear   INV 25-NOV-2025
DEFINITION  annexin-B12-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505614
VERSION     XP_077505614.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649488.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..545
                     /product="annexin-B12-like isoform X1"
                     /calculated_mol_wt=59583
     Region          <62..214
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          245..310
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          317..382
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          401..466
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          476..541
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     CDS             1..545
                     /gene="LOC144115211"
                     /coded_by="XM_077649488.1:140..1777"
                     /db_xref="GeneID:144115211"
ORIGIN      
        1 msgssggasg gynpgcapgy ppgypptsss syptdavrpr gfvdpeeppr rmpsprpsfp
       61 ersatpavgn vssslepvsp etyytpqsgg tptdlsisat flsplpetyc ppsqssglpp
      121 egisspisls pipgpfqhrt wgvaptplpr yssgvyppya tpplsaqsmy gsrsgysgsg
      181 ysrcdyagpv sgmpyppttp ntapardaay yetspryydg tvtgtscyap gkgtlkthmn
      241 fnvqqdveal kkamrgwttd haaiidilca rsepqrqeiv ttykqsfrrd lvedakssfr
      301 gdlediivgl lyplheylar elrkaiaglg tdeeclveil ctqsnqdirl ikdhyqrifk
      361 kdlekdvigd tsgafrrllv smcnaqreen tpldpvrarn daaelhkagv qrwgtdvsaf
      421 nriiatqsye qlrlvfreyn avanhtvmea iksemsgdlk ngflaivkcv ysppfyfaet
      481 lyhamkglgt ndrvlkrvvv srcekdleli kqeyldqykv slasaiqsdt sghyrkalla
      541 ivqgn
//
LOCUS       XP_077505615             526 aa            linear   INV 25-NOV-2025
DEFINITION  annexin-B12-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505615
VERSION     XP_077505615.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649489.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..526
                     /product="annexin-B12-like isoform X2"
                     /calculated_mol_wt=57478
     Region          <62..214
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          245..310
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          317..382
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          395..447
                     /region_name="ANX"
                     /note="Annexin repeats; smart00335"
                     /db_xref="CDD:197661"
     Region          457..522
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     CDS             1..526
                     /gene="LOC144115211"
                     /coded_by="XM_077649489.1:140..1720"
                     /db_xref="GeneID:144115211"
ORIGIN      
        1 msgssggasg gynpgcapgy ppgypptsss syptdavrpr gfvdpeeppr rmpsprpsfp
       61 ersatpavgn vssslepvsp etyytpqsgg tptdlsisat flsplpetyc ppsqssglpp
      121 egisspisls pipgpfqhrt wgvaptplpr yssgvyppya tpplsaqsmy gsrsgysgsg
      181 ysrcdyagpv sgmpyppttp ntapardaay yetspryydg tvtgtscyap gkgtlkthmn
      241 fnvqqdveal kkamrgwttd haaiidilca rsepqrqeiv ttykqsfrrd lvedakssfr
      301 gdlediivgl lyplheylar elrkaiaglg tdeeclveil ctqsnqdirl ikdhyqrifk
      361 kdlekdvigd tsgafrrllv smcnelhkag vqrwgtdvsa fnriiatqsy eqlrlvfrey
      421 navanhtvme aiksemsgdl kngflaivkc vysppfyfae tlyhamkglg tndrvlkrvv
      481 vsrcekdlel ikqeyldqyk vslasaiqsd tsghyrkall aivqgn
//
LOCUS       XP_077505616             550 aa            linear   INV 25-NOV-2025
DEFINITION  annexin-B12-like [Amblyomma americanum].
ACCESSION   XP_077505616
VERSION     XP_077505616.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649490.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..550
                     /product="annexin-B12-like"
                     /calculated_mol_wt=59459
     Region          <60..219
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          250..315
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          322..387
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          406..471
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     Region          481..546
                     /region_name="Annexin"
                     /note="pfam00191"
                     /db_xref="CDD:395139"
     CDS             1..550
                     /gene="LOC144115212"
                     /coded_by="XM_077649490.1:281..1933"
                     /db_xref="GeneID:144115212"
ORIGIN      
        1 msgssggasg ggynpgcapg yppgypptss ssyptdavrp mgfvdpeepp rrmpsprpsf
       61 pepsvtpavg ntssvlepvs retyyppsqs vgaptgcsps atslsnvpgt syppsesssl
      121 ppegipspis lspisgplqs gpvppwavas tplarypsgv yppysttpls aqsiygsrng
      181 ysgsgysrsd yagpgsgmpy ppttpsrapa rdaacyetsp ryydgmvaga scyapgkgtl
      241 kthmnfnvqq dvealkkamr gwttdhgaii dilcarsepq rqeivttykq sfgrdlveda
      301 ksalrgdled iivgllyplh eylarelrka iaglgtdeec lveilctqsn qdirlikdhy
      361 qrifkkdlek dvigdtsgdf rrllismcna qrdentpldp vrarndaael hkagvqrwgt
      421 dvstfnriia tqsyeqlrlv freynavanh tvmeaiksem sgdlkngfla ivkcvysppf
      481 yfaetlyham kglgtndrvl krvvvsrcek dlelikqeyl dkykvtlasa iqsdtsgnyr
      541 kallaivqgn
//
LOCUS       XP_077505617             767 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077505617
VERSION     XP_077505617.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649491.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..767
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=84858
     Region          83..210
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          306..694
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..767
                     /gene="LOC144115213"
                     /coded_by="XM_077649491.1:78..2381"
                     /db_xref="GeneID:144115213"
ORIGIN      
        1 msaqriagal lllcacsstl ltfvsaaanv ettssttstt psaptstfks lgadlddvak
       61 vlvskflpsv sdviaspels vacsasiakl llavrnkdaw aikmllsngf lpsnlfeggl
      121 vnlgsyeqcl rtsavnrvge ktirgqycsl hfkppveafk sllarfnevg elmgrenplt
      181 rtsrekfast dfrvgictps tcseedlqvv trkmlgqygf dstvrscrtd spknitklqa
      241 asmavlgtvv ltvvaatlte wvvqaraagt vkrkdgtalg tllyfsalsn tryllrtent
      301 eknrpllflg gfkvvlilwv iyghsyimvq lefaqdlfei gavfrkvasq iipngflsvs
      361 tflylsgfal tfsllgfrqg lykqnllvvf iigcckryir ltipvigvil gtfllpllvd
      421 gpadeefvgs evqgcitnww tvfvqannfn smddmclqhl wyvsadmqlf ffmalpltli
      481 fvrhpkigfa lsaaaavgft vlttlqiyyw dvaysmtlat ndqrktylsl eliyykpfth
      541 vgtyvvglvt gylaleyrnk pihkavqaaq wlmsiglacf vmfvtvhwnr gnppsdvvna
      601 lyggfhrllw alalawpsfa catgrggiln gflswkalrp lsrltyctyl vhlwfflirm
      661 gslktyfdlg eyvqlmtslg ifcysvffgy llylcfeapg yhlqrilfni ptsskesrek
      721 sddhsgtevd iekasckinn sdhggksrnl kpvdnpgfet daeksrl
//
LOCUS       XP_077505618             717 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077505618
VERSION     XP_077505618.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649492.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..717
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=79235
     Region          67..194
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          304..640
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..717
                     /gene="LOC144115214"
                     /coded_by="XM_077649492.1:1..2154"
                     /db_xref="GeneID:144115214"
ORIGIN      
        1 maaalrallc vllvgsaass yagpapyagt asknmmddfe rlihslvprv mpavselvgs
       61 tnvstacaag lfkaflairk hepwvlrmll sngllpanil egslvsvgsy sqclktrvld
      121 dqgritfkgq ycslsvkpar lllrqlvery kmaaeltdty slvngttnek vsnpnlrlgi
      181 ctpstcsand lqfittklva pygittevkg crtndpkkmt tlqagtvafl cslaslvvla
      241 tavewllmrr pvarkkhepe vplrillmfs aiknmrallr tavhdnplnf vaglkvllvl
      301 wvvyghafii mpiefaddlf alldifskts aqvlpnavls vstffflsgf vlpfavpnpk
      361 nptrrraigt vylmgvikry irvmlpsmal ilcfylvpll vdgpadqdsl alhvhnciqk
      421 pwvvpalinn flplhlmcln hlwyvcadlq ifiviglpma lmilrypkts svlscavalf
      481 ccgltsyqiy kwgvtysvtl gthdaslsls hleyiyvrpf chvvsyvvgi lagylsvsht
      541 svslnrwmqt alwllslals sfvmfvtvpw nrgnlpsaav nalyggfqrl lwslallwpc
      601 yacatgyggw lnsfmswtaf rplsrltfci ylthvpmfyi ragnmrtnfe lseffqlnas
      661 vgiywfsvvf glllhlcvea pvahlqkflf erdsgagrts aeakpspven geqrskl
//
LOCUS       XP_077505619             777 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077505619
VERSION     XP_077505619.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649493.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..777
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=87121
     Region          66..194
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          292..680
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..777
                     /gene="LOC144115216"
                     /coded_by="XM_077649493.1:505..2838"
                     /db_xref="GeneID:144115216"
ORIGIN      
        1 mnpalllvaa sllldrtwnn apfvcgeeta tksleteltd iggmlmkklm pvaselatsp
       61 elspecagsl fkvlkatrtr epwvlrmila nglipnnlle gslislggye qclktrvyns
      121 egelvhkgqy cslfvyppws lletfvkkfq avgefsgrln pleittnski grkalriglc
      181 tldacsedev nflaagvfnq yganttvtgc rnddpkeftk lqkasiiyls vlgllvvigt
      241 liewiliklp apdkeanllr vwaplklfmf fsaisntqrl lktearaenk nlvflsgfki
      301 fliiwviyah cyvliqpefa rspfvfvdls gkvlfqlmln gllsvstflf lsgfvmsylm
      361 lqsrsilrkq npiaiyiiaa vrryfrlilp ivsvvmacfl lpyfadgpan hellqqqvng
      421 cidrwwavlf sfnnfypldk mcmihlwyvs tdvqiflvis fpltilfvrh rtlallmsgv
      481 islafcilvs lqthfwhlly sgtaasndvv rlvdtielvy frpfthvaty vlgvvigyia
      541 vdygkakigi vmqfaqwiis lalcgfvlfi tlpwnrgnpp ddittavyag fhrlawslgl
      601 vwptyacatg rggllnaffs wkafiplsrl vygvylvhvp llylrmgmik thinlneffq
      661 lntaiglfgm slflayllyl steapvqrfe rlvfegkrkd aimnaakkpp afpqakmaiv
      721 evetkkvpps ddvekkpafd gqpevkltie ndatktalkq agtdnqafqe esekskl
//
LOCUS       XP_077505620             341 aa            linear   INV 25-NOV-2025
DEFINITION  cyclin-D1-binding protein 1 homolog [Amblyomma americanum].
ACCESSION   XP_077505620
VERSION     XP_077505620.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649494.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..341
                     /product="cyclin-D1-binding protein 1 homolog"
                     /calculated_mol_wt=38436
     Region          65..304
                     /region_name="GCIP"
                     /note="Grap2 and cyclin-D-interacting; pfam13324"
                     /db_xref="CDD:463844"
     CDS             1..341
                     /gene="LOC144115217"
                     /coded_by="XM_077649494.1:241..1266"
                     /db_xref="GeneID:144115217"
ORIGIN      
        1 maekaaiatl eetlqevsrk lkedhgqmpe gaedfdkatf wrrllqevgq crddmkqvcl
       61 aceklspqeh leqelidrlk rgcqevvsaf lelpqsqgmt lrnelavalg dlfstlgrfa
      121 qevslqrnqd gagdstvcsi kqaaasclek feaiekvskd nkeavlsvlt lhyevvedav
      181 qelsealaee qqivevdldq lpvrngftqr rrftltpenr gfldpglglv kavqiamrkt
      241 kaavdlrgqc tnreeveeld kmaglyllss syidefitsl yhpvvthavi enasdlraha
      301 qailrtirqs hfhsrtddwi sfmesaiehn frnviercem q
//
LOCUS       XP_077505621             309 aa            linear   INV 25-NOV-2025
DEFINITION  neuroguidin-like [Amblyomma americanum].
ACCESSION   XP_077505621
VERSION     XP_077505621.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649495.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..309
                     /product="neuroguidin-like"
                     /calculated_mol_wt=34772
     Region          20..100
                     /region_name="Sas10_Utp3"
                     /note="Sas10/Utp3/C1D family; pfam04000"
                     /db_xref="CDD:461123"
     CDS             1..309
                     /gene="LOC144115218"
                     /coded_by="XM_077649495.1:181..1110"
                     /db_xref="GeneID:144115218"
ORIGIN      
        1 msqiagpdqh gklpdflalf kevkekasva svaveslasr agskelntea glsflemknh
       61 vmlsylldlt hviwckvsgk sisgdpsirr lvqartvler irpidqklky qidkivktat
      121 tgtvdaddpl rfranpaale aesgdestgt iddekgeqdk katkvyrppk lapvhydgde
      181 tekerrerll erakrkalst svmeelrnef fdgpieikda ydshkakqnk alqervqyee
      241 dnmlrlslsk kemnlgrqfg tmsslkdlts fgdfsaldad dvddlrppkk akkvykkkwg
      301 kkgfrkgrr
//
LOCUS       XP_077505622             585 aa            linear   INV 25-NOV-2025
DEFINITION  CBFA2/RUNX1 partner transcriptional co-repressor nervy isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077505622
VERSION     XP_077505622.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649496.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..585
                     /product="CBFA2/RUNX1 partner transcriptional co-repressor
                     nervy isoform X1"
                     /calculated_mol_wt=63241
     Region          140..229
                     /region_name="TAFH"
                     /note="NHR1 homology to TAF; pfam07531"
                     /db_xref="CDD:462195"
     Region          365..>403
                     /region_name="NHR2"
                     /note="NHR2 domain like; pfam08788"
                     /db_xref="CDD:430219"
     Region          <425..505
                     /region_name="AtpF"
                     /note="FoF1-type ATP synthase, membrane subunit b or b'
                     [Energy production and conversion]; COG0711"
                     /db_xref="CDD:440475"
     Region          516..552
                     /region_name="zf-MYND"
                     /note="MYND finger; pfam01753"
                     /db_xref="CDD:460312"
     CDS             1..585
                     /gene="nvy"
                     /coded_by="XM_077649496.1:230..1987"
                     /db_xref="GeneID:144115219"
ORIGIN      
        1 mygalgifrw kgpdrcylaa tttpslgsgg pssqhlgckm pdspqdikpl sptqlpkvss
       61 mqgvngahsp pcnagnpvpv cpnpaavrsl sppsdsnhgs gstgvsssta asnvsggtan
      121 iasvsglvea psgvvlgarq lskvkrfltt lqqfasdvsp dvgervhgli mglvsstisi
      181 edfhhsiqdi tnyplrpfvv pflkshlpll qtelvhyara akqspaqylr qheqlvldqp
      241 ggepfeifqp dakegaplkr rtppdgrpke nghaasavaa gaavaldegg ppaakrhqpl
      301 ssspgaarls pgvgghaahp ppaafrfeds saplfrdryd rydrldrdfy rpcygmlhrd
      361 ypeerdmede wknintmlnc ilgmvektkr aiailqhrsq aerpdfsasv wtgsrrhldv
      421 dydlkkrdlm ahyksaaeid rvsevrrrae eavnevkrqa vaelqkavsa aeskaselva
      481 aertkmerll gearrqaaee alaavnhqed steqncwncg rkanetcsgc nvarycgafc
      541 qhkdwenhhr vcgqpsssps ssggpvgkrs ptpprrlegk vdarh
//
LOCUS       XP_077505623             584 aa            linear   INV 25-NOV-2025
DEFINITION  CBFA2/RUNX1 partner transcriptional co-repressor nervy isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077505623
VERSION     XP_077505623.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649497.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..584
                     /product="CBFA2/RUNX1 partner transcriptional co-repressor
                     nervy isoform X2"
                     /calculated_mol_wt=63113
     Region          140..229
                     /region_name="TAFH"
                     /note="NHR1 homology to TAF; pfam07531"
                     /db_xref="CDD:462195"
     Region          365..>403
                     /region_name="NHR2"
                     /note="NHR2 domain like; pfam08788"
                     /db_xref="CDD:430219"
     Region          <425..505
                     /region_name="AtpF"
                     /note="FoF1-type ATP synthase, membrane subunit b or b'
                     [Energy production and conversion]; COG0711"
                     /db_xref="CDD:440475"
     Region          515..551
                     /region_name="zf-MYND"
                     /note="MYND finger; pfam01753"
                     /db_xref="CDD:460312"
     CDS             1..584
                     /gene="nvy"
                     /coded_by="XM_077649497.1:230..1984"
                     /db_xref="GeneID:144115219"
ORIGIN      
        1 mygalgifrw kgpdrcylaa tttpslgsgg pssqhlgckm pdspqdikpl sptqlpkvss
       61 mqgvngahsp pcnagnpvpv cpnpaavrsl sppsdsnhgs gstgvsssta asnvsggtan
      121 iasvsglvea psgvvlgarq lskvkrfltt lqqfasdvsp dvgervhgli mglvsstisi
      181 edfhhsiqdi tnyplrpfvv pflkshlpll qtelvhyara akqspaqylr qheqlvldqp
      241 ggepfeifqp dakegaplkr rtppdgrpke nghaasavaa gaavaldegg ppaakrhqpl
      301 ssspgaarls pgvgghaahp ppaafrfeds saplfrdryd rydrldrdfy rpcygmlhrd
      361 ypeerdmede wknintmlnc ilgmvektkr aiailqhrsq aerpdfsasv wtgsrrhldv
      421 dydlkkrdlm ahyksaaeid rvsevrrrae eavnevkrqa vaelqkavsa aeskaselva
      481 aertkmerll gearrqaaee alaavnhqed stencwncgr kanetcsgcn varycgafcq
      541 hkdwenhhrv cgqpssspss sggpvgkrsp tpprrlegkv darh
//
LOCUS       XP_077505624             581 aa            linear   INV 25-NOV-2025
DEFINITION  CBFA2/RUNX1 partner transcriptional co-repressor nervy isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077505624
VERSION     XP_077505624.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649498.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..581
                     /product="CBFA2/RUNX1 partner transcriptional co-repressor
                     nervy isoform X3"
                     /calculated_mol_wt=62716
     Region          136..225
                     /region_name="TAFH"
                     /note="NHR1 homology to TAF; pfam07531"
                     /db_xref="CDD:462195"
     Region          361..>399
                     /region_name="NHR2"
                     /note="NHR2 domain like; pfam08788"
                     /db_xref="CDD:430219"
     Region          <421..501
                     /region_name="AtpF"
                     /note="FoF1-type ATP synthase, membrane subunit b or b'
                     [Energy production and conversion]; COG0711"
                     /db_xref="CDD:440475"
     Region          512..548
                     /region_name="zf-MYND"
                     /note="MYND finger; pfam01753"
                     /db_xref="CDD:460312"
     CDS             1..581
                     /gene="nvy"
                     /coded_by="XM_077649498.1:620..2365"
                     /db_xref="GeneID:144115219"
ORIGIN      
        1 mamgyplynv geylaatttp slgsggpssq hlgckmpdsp qdikplsptq lpkvssmqgv
       61 ngahsppcna gnpvpvcpnp aavrslspps dsnhgsgstg vssstaasnv sggtaniasv
      121 sglveapsgv vlgarqlskv krflttlqqf asdvspdvge rvhglimglv sstisiedfh
      181 hsiqditnyp lrpfvvpflk shlpllqtel vhyaraakqs paqylrqheq lvldqpggep
      241 feifqpdake gaplkrrtpp dgrpkengha asavaagaav aldeggppaa krhqplsssp
      301 gaarlspgvg ghaahpppaa frfedssapl frdrydrydr ldrdfyrpcy gmlhrdypee
      361 rdmedewkni ntmlncilgm vektkraiai lqhrsqaerp dfsasvwtgs rrhldvdydl
      421 kkrdlmahyk saaeidrvse vrrraeeavn evkrqavael qkavsaaesk aselvaaert
      481 kmerllgear rqaaeealaa vnhqedsteq ncwncgrkan etcsgcnvar ycgafcqhkd
      541 wenhhrvcgq pssspsssgg pvgkrsptpp rrlegkvdar h
//
LOCUS       XP_077505625             546 aa            linear   INV 25-NOV-2025
DEFINITION  CBFA2/RUNX1 partner transcriptional co-repressor nervy isoform X4
            [Amblyomma americanum].
ACCESSION   XP_077505625
VERSION     XP_077505625.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649499.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..546
                     /product="CBFA2/RUNX1 partner transcriptional co-repressor
                     nervy isoform X4"
                     /calculated_mol_wt=59174
     Region          101..190
                     /region_name="TAFH"
                     /note="NHR1 homology to TAF; pfam07531"
                     /db_xref="CDD:462195"
     Region          326..>364
                     /region_name="NHR2"
                     /note="NHR2 domain like; pfam08788"
                     /db_xref="CDD:430219"
     Region          <386..466
                     /region_name="AtpF"
                     /note="FoF1-type ATP synthase, membrane subunit b or b'
                     [Energy production and conversion]; COG0711"
                     /db_xref="CDD:440475"
     Region          477..513
                     /region_name="zf-MYND"
                     /note="MYND finger; pfam01753"
                     /db_xref="CDD:460312"
     CDS             1..546
                     /gene="nvy"
                     /coded_by="XM_077649499.1:824..2464"
                     /db_xref="GeneID:144115219"
ORIGIN      
        1 mpdspqdikp lsptqlpkvs smqgvngahs ppcnagnpvp vcpnpaavrs lsppsdsnhg
       61 sgstgvssst aasnvsggta niasvsglve apsgvvlgar qlskvkrflt tlqqfasdvs
      121 pdvgervhgl imglvsstis iedfhhsiqd itnyplrpfv vpflkshlpl lqtelvhyar
      181 aakqspaqyl rqheqlvldq pggepfeifq pdakegaplk rrtppdgrpk enghaasava
      241 agaavaldeg gppaakrhqp lssspgaarl spgvgghaah pppaafrfed ssaplfrdry
      301 drydrldrdf yrpcygmlhr dypeerdmed ewknintmln cilgmvektk raiailqhrs
      361 qaerpdfsas vwtgsrrhld vdydlkkrdl mahyksaaei drvsevrrra eeavnevkrq
      421 avaelqkavs aaeskaselv aaertkmerl lgearrqaae ealaavnhqe dsteqncwnc
      481 grkanetcsg cnvarycgaf cqhkdwenhh rvcgqpsssp sssggpvgkr sptpprrleg
      541 kvdarh
//
LOCUS       XP_077505626             545 aa            linear   INV 25-NOV-2025
DEFINITION  CBFA2/RUNX1 partner transcriptional co-repressor nervy isoform X5
            [Amblyomma americanum].
ACCESSION   XP_077505626
VERSION     XP_077505626.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649500.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..545
                     /product="CBFA2/RUNX1 partner transcriptional co-repressor
                     nervy isoform X5"
                     /calculated_mol_wt=59045
     Region          101..190
                     /region_name="TAFH"
                     /note="NHR1 homology to TAF; pfam07531"
                     /db_xref="CDD:462195"
     Region          326..>364
                     /region_name="NHR2"
                     /note="NHR2 domain like; pfam08788"
                     /db_xref="CDD:430219"
     Region          <386..466
                     /region_name="AtpF"
                     /note="FoF1-type ATP synthase, membrane subunit b or b'
                     [Energy production and conversion]; COG0711"
                     /db_xref="CDD:440475"
     Region          476..512
                     /region_name="zf-MYND"
                     /note="MYND finger; pfam01753"
                     /db_xref="CDD:460312"
     CDS             1..545
                     /gene="nvy"
                     /coded_by="XM_077649500.1:820..2457"
                     /db_xref="GeneID:144115219"
ORIGIN      
        1 mpdspqdikp lsptqlpkvs smqgvngahs ppcnagnpvp vcpnpaavrs lsppsdsnhg
       61 sgstgvssst aasnvsggta niasvsglve apsgvvlgar qlskvkrflt tlqqfasdvs
      121 pdvgervhgl imglvsstis iedfhhsiqd itnyplrpfv vpflkshlpl lqtelvhyar
      181 aakqspaqyl rqheqlvldq pggepfeifq pdakegaplk rrtppdgrpk enghaasava
      241 agaavaldeg gppaakrhqp lssspgaarl spgvgghaah pppaafrfed ssaplfrdry
      301 drydrldrdf yrpcygmlhr dypeerdmed ewknintmln cilgmvektk raiailqhrs
      361 qaerpdfsas vwtgsrrhld vdydlkkrdl mahyksaaei drvsevrrra eeavnevkrq
      421 avaelqkavs aaeskaselv aaertkmerl lgearrqaae ealaavnhqe dstencwncg
      481 rkanetcsgc nvarycgafc qhkdwenhhr vcgqpsssps ssggpvgkrs ptpprrlegk
      541 vdarh
//
LOCUS       XP_077505627             546 aa            linear   INV 25-NOV-2025
DEFINITION  CBFA2/RUNX1 partner transcriptional co-repressor nervy isoform X4
            [Amblyomma americanum].
ACCESSION   XP_077505627
VERSION     XP_077505627.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649501.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..546
                     /product="CBFA2/RUNX1 partner transcriptional co-repressor
                     nervy isoform X4"
                     /calculated_mol_wt=59174
     Region          101..190
                     /region_name="TAFH"
                     /note="NHR1 homology to TAF; pfam07531"
                     /db_xref="CDD:462195"
     Region          326..>364
                     /region_name="NHR2"
                     /note="NHR2 domain like; pfam08788"
                     /db_xref="CDD:430219"
     Region          <386..466
                     /region_name="AtpF"
                     /note="FoF1-type ATP synthase, membrane subunit b or b'
                     [Energy production and conversion]; COG0711"
                     /db_xref="CDD:440475"
     Region          477..513
                     /region_name="zf-MYND"
                     /note="MYND finger; pfam01753"
                     /db_xref="CDD:460312"
     CDS             1..546
                     /gene="nvy"
                     /coded_by="XM_077649501.1:207..1847"
                     /db_xref="GeneID:144115219"
ORIGIN      
        1 mpdspqdikp lsptqlpkvs smqgvngahs ppcnagnpvp vcpnpaavrs lsppsdsnhg
       61 sgstgvssst aasnvsggta niasvsglve apsgvvlgar qlskvkrflt tlqqfasdvs
      121 pdvgervhgl imglvsstis iedfhhsiqd itnyplrpfv vpflkshlpl lqtelvhyar
      181 aakqspaqyl rqheqlvldq pggepfeifq pdakegaplk rrtppdgrpk enghaasava
      241 agaavaldeg gppaakrhqp lssspgaarl spgvgghaah pppaafrfed ssaplfrdry
      301 drydrldrdf yrpcygmlhr dypeerdmed ewknintmln cilgmvektk raiailqhrs
      361 qaerpdfsas vwtgsrrhld vdydlkkrdl mahyksaaei drvsevrrra eeavnevkrq
      421 avaelqkavs aaeskaselv aaertkmerl lgearrqaae ealaavnhqe dsteqncwnc
      481 grkanetcsg cnvarycgaf cqhkdwenhh rvcgqpsssp sssggpvgkr sptpprrleg
      541 kvdarh
//
LOCUS       XP_077505628             135 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115221 [Amblyomma americanum].
ACCESSION   XP_077505628
VERSION     XP_077505628.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649502.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..135
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..135
                     /product="uncharacterized protein LOC144115221"
                     /calculated_mol_wt=14736
     CDS             1..135
                     /gene="LOC144115221"
                     /coded_by="XM_077649502.1:16..423"
                     /db_xref="GeneID:144115221"
ORIGIN      
        1 maclsvrrsq dlldlkllgl rletkltile gqlnklsats skcnqggrtg gpdsdtdvat
       61 sdtqerilra qqaiidisnr ntvelenlsq lvttqtakla nrsdamlgll ksselvhrrp
      121 ppqetisqgr rhhqp
//
LOCUS       XP_077505629             418 aa            linear   INV 25-NOV-2025
DEFINITION  lysosomal phospholipase A and acyltransferase-like [Amblyomma
            americanum].
ACCESSION   XP_077505629
VERSION     XP_077505629.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649503.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..418
                     /product="lysosomal phospholipase A and
                     acyltransferase-like"
                     /calculated_mol_wt=47311
     Region          77..405
                     /region_name="Abhydrolase"
                     /note="alpha/beta hydrolases; cl21494"
                     /db_xref="CDD:473884"
     CDS             1..418
                     /gene="LOC144115223"
                     /coded_by="XM_077649503.1:186..1442"
                     /db_xref="GeneID:144115223"
ORIGIN      
        1 msparkvall siacavlcve daagatvfer mwnqilpvkr hpvilvpgdg gsqleaklnk
       61 tetvhyfcer rtadyfdlwv nlelmvpyvl dcwvdnmrlv ydnktrttrs pdgvdvrvpg
      121 fgntstvewl dpsqvsptay ftriveelll lgyqrgvdvr gapydfrkap nelgdyferl
      181 hdlieetfst ngdqsvilvc hsmgcpnlry ffsrqrqawk dkhiralvtl ggawggavka
      241 lkayasgenl gvvvinplsv rpeqrstpsl aymlpnrlyw nesevlvktl drsygladyk
      301 affddigfpd gyemykdtrp ymldntapgv eihclhgrnv stierieyhk pgfpdiqpqv
      361 vfgdgdgtvn lrslrgclrf qygqnqrvye ktldnvehms ilyepaviey ikalvlgq
//
LOCUS       XP_077505630             126 aa            linear   INV 25-NOV-2025
DEFINITION  nuclear envelope phosphatase-regulatory subunit 1-like [Amblyomma
            americanum].
ACCESSION   XP_077505630
VERSION     XP_077505630.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649504.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..126
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..126
                     /product="nuclear envelope phosphatase-regulatory subunit
                     1-like"
                     /calculated_mol_wt=14275
     Region          6..123
                     /region_name="Tmemb_18A"
                     /note="Transmembrane protein 188; pfam09771"
                     /db_xref="CDD:401646"
     CDS             1..126
                     /gene="LOC144115224"
                     /coded_by="XM_077649504.1:251..631"
                     /db_xref="GeneID:144115224"
ORIGIN      
        1 msleqtaced lkaferrlte vigylnpqtk rwriilfiss ictaigawqw lmdpvtsqat
       61 fvqslmnhmf ftissiilvi lflmgihkrv vtpsiivsrv rnvlsdfnms cddggrlilr
      121 prptts
//
LOCUS       XP_077505631             135 aa            linear   INV 25-NOV-2025
DEFINITION  mediator complex subunit 10 [Amblyomma americanum].
ACCESSION   XP_077505631
VERSION     XP_077505631.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649505.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..135
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..135
                     /product="mediator complex subunit 10"
                     /calculated_mol_wt=15498
     Region          9..126
                     /region_name="Med10"
                     /note="Transcription factor subunit Med10 of Mediator
                     complex; pfam09748"
                     /db_xref="CDD:462879"
     CDS             1..135
                     /gene="MED10"
                     /coded_by="XM_077649505.1:131..538"
                     /db_xref="GeneID:144115225"
ORIGIN      
        1 mssplealet nlemfienvr qlgiivsdfq pqgqttlnqk inhivtlmqe vdrckpqvqd
       61 iqvplevfdy idqgrnpqlf tkdcmekalt kneqvkgkie syrrfkalll lelskvfpte
      121 makyrairgd erpat
//
LOCUS       XP_077505632             700 aa            linear   INV 25-NOV-2025
DEFINITION  nuclear localized protein 1 [Amblyomma americanum].
ACCESSION   XP_077505632
VERSION     XP_077505632.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649506.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..700
                     /product="nuclear localized protein 1"
                     /calculated_mol_wt=78014
     Region          247..586
                     /region_name="Tcf25"
                     /note="Transcriptional repressor TCF25; pfam04910"
                     /db_xref="CDD:461476"
     CDS             1..700
                     /gene="Nulp1"
                     /coded_by="XM_077649506.1:242..2344"
                     /db_xref="GeneID:144115226"
ORIGIN      
        1 msnrvlkklq gvndigklda qsetedetpn vaasdarknr qaaanrfell tglsaeqsls
       61 dtakedddre nepppargsa agapqesall gdeaprskle kqdstqstds lkrrkkkkkk
      121 kkqlnsqhsh edrlhhtede vaasvrevnr mlgepenlph vaavtgtvcg ptrpeksllt
      181 vdqrnlnpen emrrifgsrv vqsekrrgrg rvygrtailv pgrnwrhigk pgismelvet
      241 qgdvhfftfe hsksyqaiqf qfldavetfn pdnivallnq hpyhvdsliq fsdvckmsed
      301 lqmaaelier alyclescfh mlfnvtqgnc rldyrrsenr slflalfkhl nfvgqrgcsr
      361 talefcklll gldpendplc vtllldyyai radqyqylvr lyhewdaern lcqlpnfafs
      421 vplalfhlar gsaseggvst tqqeadkmlq eslimfpgvl mplldkcgvq pdsevtkhgf
      481 faaaaraqqp aalnqlqslf vgrnyvlwke pevlawlern vravlkrvdq adplvtssae
      541 krmkryqgap rnlcrhiils niqeasvtlp qelsnspifg fdplppadai vsytrppraq
      601 qigdqsmmga ffrsllpsfn lpeevvgidv qnpmvlvepg paeqgavgaa eadgvnadaa
      661 dlrrsvtsll eamrdllssi hlpsdvpndg dvdsddehed
//
LOCUS       XP_077505633             356 aa            linear   INV 25-NOV-2025
DEFINITION  seipin-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505633
VERSION     XP_077505633.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649507.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..356
                     /product="seipin-like isoform X1"
                     /calculated_mol_wt=39879
     Region          35..233
                     /region_name="Seipin"
                     /note="Putative adipose-regulatory protein (Seipin);
                     pfam06775"
                     /db_xref="CDD:462007"
     CDS             1..356
                     /gene="LOC144115228"
                     /coded_by="XM_077649507.1:318..1388"
                     /db_xref="GeneID:144115228"
ORIGIN      
        1 mifallklpl avfggvrqlv sqsffvavll svitwtsill ygvfywnyip ksshvfpvhl
       61 hfeskacpgg ycdypvanvt ivrpgqddyl argqrykiyl elempesean qrigmftvrl
      121 dmisedgevl rstqrsgvlr yksvlvrlfs tlfyipmlll gsaeekqtvs vllferymed
      181 yakpatqagi mirnshievy tatlkiyadf tglryllynw pltsavvgis tnfffvslva
      241 llswnhlapa kpkprrsiad kkkhpsngkk tleeqvdaae ddtasdisgn qpdliketve
      301 akkvrkrkel dektgktlre lfgattqgsd aslssldgdq iasgdasprr lekept
//
LOCUS       XP_077505634             315 aa            linear   INV 25-NOV-2025
DEFINITION  seipin-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505634
VERSION     XP_077505634.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649508.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..315
                     /product="seipin-like isoform X2"
                     /calculated_mol_wt=35778
     Region          35..233
                     /region_name="Seipin"
                     /note="Putative adipose-regulatory protein (Seipin);
                     pfam06775"
                     /db_xref="CDD:462007"
     CDS             1..315
                     /gene="LOC144115228"
                     /coded_by="XM_077649508.1:318..1265"
                     /db_xref="GeneID:144115228"
ORIGIN      
        1 mifallklpl avfggvrqlv sqsffvavll svitwtsill ygvfywnyip ksshvfpvhl
       61 hfeskacpgg ycdypvanvt ivrpgqddyl argqrykiyl elempesean qrigmftvrl
      121 dmisedgevl rstqrsgvlr yksvlvrlfs tlfyipmlll gsaeekqtvs vllferymed
      181 yakpatqagi mirnshievy tatlkiyadf tglryllynw pltsavvgis tnfffvslva
      241 llswnhlapa kpkprrsiad kkkhpsngkk tleeqvdaae ddtasdisgn qpdliketve
      301 akkvrkrkda wtwwn
//
LOCUS       XP_077505635             338 aa            linear   INV 25-NOV-2025
DEFINITION  gamma Soluble NSF attachment protein 1 [Amblyomma americanum].
ACCESSION   XP_077505635
VERSION     XP_077505635.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649509.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..338
                     /product="gamma Soluble NSF attachment protein 1"
                     /calculated_mol_wt=36496
     Region          15..255
                     /region_name="SNAP"
                     /note="Soluble N-ethylmaleimide-sensitive factor (NSF)
                     Attachment Protein family; cl24038"
                     /db_xref="CDD:451671"
     Region          37..74
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276937"
     Region          78..115
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276937"
     Region          118..153
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276937"
     Region          157..187
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276937"
     CDS             1..338
                     /gene="gammaSnap1"
                     /coded_by="XM_077649509.1:155..1171"
                     /db_xref="GeneID:144115230"
ORIGIN      
        1 mgtpvnssas rkiaegteal rqaekclktg llkwkpdlds aanefskaat cfkaakaldq
       61 ckdahlraad cylrngsffs sgkqleqaal vsrdmgnlqa aaelidrasr lyidsrspdt
      121 aalvlergak ivesklpeva veffnrasei vsvedrprqs aefcghavrl llklsrwdea
      181 aeaisrqkql lteaqderav grlvvalvlv hlarddfvaa skaldhdggf leaeeqdtls
      241 cllkgcdegd pdmvshslna pflkhmdtey aklarmlgqq vaesakktas esqpaaetrh
      301 vaqdtdaetv akpdqdhlev pesaaagdse deyaggll
//
LOCUS       XP_077505636             293 aa            linear   INV 25-NOV-2025
DEFINITION  plasmanylethanolamine desaturase 1-like [Amblyomma americanum].
ACCESSION   XP_077505636
VERSION     XP_077505636.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649510.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..293
                     /product="plasmanylethanolamine desaturase 1-like"
                     /calculated_mol_wt=33291
     Region          109..285
                     /region_name="Lipid_desat"
                     /note="Lipid desaturase domain; pfam10520"
                     /db_xref="CDD:463132"
     CDS             1..293
                     /gene="LOC144115231"
                     /coded_by="XM_077649510.1:157..1038"
                     /db_xref="GeneID:144115231"
ORIGIN      
        1 mamacnllpp vktekqiyen smpeddpnsn sdtyqpsntv dgqlkprwgp hhagarelag
       61 lytrgkrtqe tvciavcltl mgynlfqlll yfqasrwsti vaaalcgvvt adflsglvhw
      121 aadtwgsvel pvigkafirp frehhidpts itrhdfietn gdnfmvpipv lasmawgfas
      181 kppqailaty hwncflflla lfvaftnqih kwshtyfglp rwvtllqewh vvlprqhhri
      241 hhvaphetyf cittgwlnyp leklrfwpal eavisaltgc rprsddlqwa lkk
//
LOCUS       XP_077505638             272 aa            linear   INV 25-NOV-2025
DEFINITION  juvenile hormone acid O-methyltransferase-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077505638
VERSION     XP_077505638.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649512.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..272
                     /product="juvenile hormone acid O-methyltransferase-like
                     isoform X1"
                     /calculated_mol_wt=30416
     Region          <33..>89
                     /region_name="UbiG"
                     /note="2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1,
                     4-benzoquinol methylase [Coenzyme transport and
                     metabolism]; COG2227"
                     /db_xref="CDD:441829"
     CDS             1..272
                     /gene="LOC144115232"
                     /coded_by="XM_077649512.1:605..1423"
                     /db_xref="GeneID:144115232"
ORIGIN      
        1 mdrvasfplp lkyiqqeyth yasgmewiaq eqvlelgcgp glisrqvlqp lceahfarll
       61 avdraseail yaqqhchhrd ityrhvpalc tsapvelrga fsklfyyggv mpcsegsglh
      121 qasllldvrg ccvlllpvrc clydvleqls gnprwkaymr davhlvppqh hwqepkeafq
      181 kqaqqagmev nsvkldttdv vfsssshfqe flswampfva ripkesrqvf ldealalsfe
      241 qgvqmtpggq lqlsvtflsa lstkmrnngl sh
//
LOCUS       XP_077505639             272 aa            linear   INV 25-NOV-2025
DEFINITION  juvenile hormone acid O-methyltransferase-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077505639
VERSION     XP_077505639.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649513.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..272
                     /product="juvenile hormone acid O-methyltransferase-like
                     isoform X1"
                     /calculated_mol_wt=30416
     Region          <33..>89
                     /region_name="UbiG"
                     /note="2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1,
                     4-benzoquinol methylase [Coenzyme transport and
                     metabolism]; COG2227"
                     /db_xref="CDD:441829"
     CDS             1..272
                     /gene="LOC144115232"
                     /coded_by="XM_077649513.1:613..1431"
                     /db_xref="GeneID:144115232"
ORIGIN      
        1 mdrvasfplp lkyiqqeyth yasgmewiaq eqvlelgcgp glisrqvlqp lceahfarll
       61 avdraseail yaqqhchhrd ityrhvpalc tsapvelrga fsklfyyggv mpcsegsglh
      121 qasllldvrg ccvlllpvrc clydvleqls gnprwkaymr davhlvppqh hwqepkeafq
      181 kqaqqagmev nsvkldttdv vfsssshfqe flswampfva ripkesrqvf ldealalsfe
      241 qgvqmtpggq lqlsvtflsa lstkmrnngl sh
//
LOCUS       XP_077505640             247 aa            linear   INV 25-NOV-2025
DEFINITION  juvenile hormone acid O-methyltransferase-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077505640
VERSION     XP_077505640.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649514.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..247
                     /product="juvenile hormone acid O-methyltransferase-like
                     isoform X2"
                     /calculated_mol_wt=27318
     Region          <8..>64
                     /region_name="UbiG"
                     /note="2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1,
                     4-benzoquinol methylase [Coenzyme transport and
                     metabolism]; COG2227"
                     /db_xref="CDD:441829"
     CDS             1..247
                     /gene="LOC144115232"
                     /coded_by="XM_077649514.1:831..1574"
                     /db_xref="GeneID:144115232"
ORIGIN      
        1 mpsaaeqvle lgcgpglisr qvlqplceah farllavdra seailyaqqh chhrdityrh
       61 vpalctsapv elrgafsklf yyggvmpcse gsglhqasll ldvrgccvll lpvrcclydv
      121 leqlsgnprw kaymrdavhl vppqhhwqep keafqkqaqq agmevnsvkl dttdvvfsss
      181 shfqeflswa mpfvaripke srqvfldeal alsfeqgvqm tpggqlqlsv tflsalstkm
      241 rnnglsh
//
LOCUS       XP_077505641             247 aa            linear   INV 25-NOV-2025
DEFINITION  juvenile hormone acid O-methyltransferase-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077505641
VERSION     XP_077505641.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649515.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..247
                     /product="juvenile hormone acid O-methyltransferase-like
                     isoform X2"
                     /calculated_mol_wt=27318
     Region          <8..>64
                     /region_name="UbiG"
                     /note="2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1,
                     4-benzoquinol methylase [Coenzyme transport and
                     metabolism]; COG2227"
                     /db_xref="CDD:441829"
     CDS             1..247
                     /gene="LOC144115232"
                     /coded_by="XM_077649515.1:170..913"
                     /db_xref="GeneID:144115232"
ORIGIN      
        1 mpsaaeqvle lgcgpglisr qvlqplceah farllavdra seailyaqqh chhrdityrh
       61 vpalctsapv elrgafsklf yyggvmpcse gsglhqasll ldvrgccvll lpvrcclydv
      121 leqlsgnprw kaymrdavhl vppqhhwqep keafqkqaqq agmevnsvkl dttdvvfsss
      181 shfqeflswa mpfvaripke srqvfldeal alsfeqgvqm tpggqlqlsv tflsalstkm
      241 rnnglsh
//
LOCUS       XP_077505642             231 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115233 [Amblyomma americanum].
ACCESSION   XP_077505642
VERSION     XP_077505642.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649516.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..231
                     /product="uncharacterized protein LOC144115233"
                     /calculated_mol_wt=25950
     Region          5..95
                     /region_name="THAP"
                     /note="The THAP domain is a putative DNA-binding domain
                     (DBD) and probably also binds a zinc ion; smart00980"
                     /db_xref="CDD:214951"
     CDS             1..231
                     /gene="LOC144115233"
                     /coded_by="XM_077649516.1:247..942"
                     /db_xref="GeneID:144115233"
ORIGIN      
        1 msalhcwavn cknsldknps lrffsfprdr tlskqwcins grgplssydr rslhrrgavl
       61 cekhfkpsqf mtpkngsdds apkqllwnai ptvfplplkt pegnepkavt lssvsrkrsr
      121 psfeeaessg eesitdvnpa ekinvenyem aalptfgrer llgiakkqwh eikrlranvr
      181 nleralassq aldetyllnv lernldgpll drvadiived fendssaaed i
//
LOCUS       XP_077505643             108 aa            linear   INV 25-NOV-2025
DEFINITION  fumarate hydratase, mitochondrial-like [Amblyomma americanum].
ACCESSION   XP_077505643
VERSION     XP_077505643.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649517.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..108
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..108
                     /product="fumarate hydratase, mitochondrial-like"
                     /calculated_mol_wt=12057
     Region          37..>77
                     /region_name="FumC"
                     /note="Fumarate hydratase class II [Energy production and
                     conversion]; COG0114"
                     /db_xref="CDD:439884"
     CDS             1..108
                     /gene="LOC144115234"
                     /coded_by="XM_077649517.1:165..491"
                     /db_xref="GeneID:144115234"
ORIGIN      
        1 mfirqiaqpg rpsawfesak tillaensfs lippnadfri eldtfgelkv psdkyygaqt
       61 mrfmlnfyig rehermpgny pldasrrnhp pigdssglak pssshgdk
//
LOCUS       XP_077505644             413 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115235 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505644
VERSION     XP_077505644.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649518.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..413
                     /product="uncharacterized protein LOC144115235 isoform X1"
                     /calculated_mol_wt=45254
     Region          329..411
                     /region_name="Dynein_light"
                     /note="Dynein light chain type 1; pfam01221"
                     /db_xref="CDD:460119"
     CDS             1..413
                     /gene="LOC144115235"
                     /coded_by="XM_077649518.1:587..1828"
                     /db_xref="GeneID:144115235"
ORIGIN      
        1 mmsmtscsgf adlksgtivi chyamglsfl alillsgrly easkeiaflr vvlaawipse
       61 adlmlggaad dhkslvldrs rqtltnlkgd leafllelts arttlalsvv fviiyilsww
      121 wmafalkqak ddgsvslrtv lllsvfaavd iaastgfvll riimyvmrdr lypmtmrtpy
      181 iteadqfaaf talrlttlks scgttdvlvs ivvgfltglr iysvacvisf ykkaknggls
      241 afrkelsnna dyfserlerl kdvedyvpkp miapmqngrh kssfyvssrp aggggsergg
      301 ilgagvggar ltcvtptast vcggeespev kiqaadmpqe mqraavesas qavklystek
      361 hvaesikqdf dqmfqptwhc vvgrnwgscv thskqcyvrm ayrdmtilly rsi
//
LOCUS       XP_077505645             406 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115235 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505645
VERSION     XP_077505645.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649519.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..406
                     /product="uncharacterized protein LOC144115235 isoform X2"
                     /calculated_mol_wt=44654
     Region          322..404
                     /region_name="Dynein_light"
                     /note="Dynein light chain type 1; pfam01221"
                     /db_xref="CDD:460119"
     CDS             1..406
                     /gene="LOC144115235"
                     /coded_by="XM_077649519.1:149..1369"
                     /db_xref="GeneID:144115235"
ORIGIN      
        1 mystdqegqk lpclyvplgl sflalillsg rlyeaskeia flrvvlaawi pseadlmlgg
       61 aaddhkslvl drsrqtltnl kgdleaflle ltsarttlal svvfviiyil swwwmafalk
      121 qakddgsvsl rtvlllsvfa avdiaastgf vllriimyvm rdrlypmtmr tpyiteadqf
      181 aaftalrltt lksscgttdv lvsivvgflt glriysvacv isfykkakng glsafrkels
      241 nnadyfserl erlkdvedyv pkpmiapmqn grhkssfyvs srpaggggse rggilgagvg
      301 garltcvtpt astvcggees pevkiqaadm pqemqraave sasqavklys tekhvaesik
      361 qdfdqmfqpt whcvvgrnwg scvthskqcy vrmayrdmti llyrsi
//
LOCUS       XP_077505646             350 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115235 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505646
VERSION     XP_077505646.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649520.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..350
                     /product="uncharacterized protein LOC144115235 isoform X3"
                     /calculated_mol_wt=38458
     Region          266..348
                     /region_name="Dynein_light"
                     /note="Dynein light chain type 1; pfam01221"
                     /db_xref="CDD:460119"
     CDS             1..350
                     /gene="LOC144115235"
                     /coded_by="XM_077649520.1:386..1438"
                     /db_xref="GeneID:144115235"
ORIGIN      
        1 mlggaaddhk slvldrsrqt ltnlkgdlea flleltsart tlalsvvfvi iyilswwwma
       61 falkqakddg svslrtvlll svfaavdiaa stgfvllrii myvmrdrlyp mtmrtpyite
      121 adqfaaftal rlttlksscg ttdvlvsivv gfltglriys vacvisfykk akngglsafr
      181 kelsnnadyf serlerlkdv edyvpkpmia pmqngrhkss fyvssrpagg ggserggilg
      241 agvggarltc vtptastvcg geespevkiq aadmpqemqr aavesasqav klystekhva
      301 esikqdfdqm fqptwhcvvg rnwgscvths kqcyvrmayr dmtillyrsi
//
LOCUS       XP_077505647             216 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115237 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505647
VERSION     XP_077505647.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649521.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..216
                     /product="uncharacterized protein LOC144115237 isoform X1"
                     /calculated_mol_wt=23241
     CDS             1..216
                     /gene="LOC144115237"
                     /coded_by="XM_077649521.1:1..651"
                     /db_xref="GeneID:144115237"
ORIGIN      
        1 mnaghkalep sgtktdaiep rastskases aariqetrea qslrnatpek gagpakkeps
       61 nltecvpgnl earsgenllp kgtqahgghg qicktlssri mqlhlccqal nlddpkmmak
      121 ippypnvdsf hkavvrnpny skqeesstss stirkhskrr qgadafksan aftgayceyd
      181 rsyepgacnf gsifpysnan vfmfmslsks alsdil
//
LOCUS       XP_077505648             213 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115237 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505648
VERSION     XP_077505648.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649522.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..213
                     /product="uncharacterized protein LOC144115237 isoform X2"
                     /calculated_mol_wt=23157
     CDS             1..213
                     /gene="LOC144115237"
                     /coded_by="XM_077649522.1:1..642"
                     /db_xref="GeneID:144115237"
ORIGIN      
        1 mnaghkalep sgtktdaiep rastskases aariqetrea qslrnatpek gagpakkeps
       61 nltecvpgnl earsgenllp kgtqahgghg qicktlssri mqlhlccqal nlddpktalp
      121 tavqvtashv ytdknqswiy rgtqaevial knkkieraii icpmvyylkn lsyphafsqk
      181 lnllqkilsp qdqipcgvis nrlkcflalf kkk
//
LOCUS       XP_077505649             208 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115237 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505649
VERSION     XP_077505649.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649523.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..208
                     /product="uncharacterized protein LOC144115237 isoform X3"
                     /calculated_mol_wt=22495
     CDS             1..208
                     /gene="LOC144115237"
                     /coded_by="XM_077649523.1:1..627"
                     /db_xref="GeneID:144115237"
ORIGIN      
        1 mnaghkalep sgtktdaiep rastskases aariqetrea qslrnatpek gagpakkeps
       61 nltecvpgnl earsgenllp kgqicktlss rimqlhlccq alnlddpkmm akippypnvd
      121 sfhkavvrnp nyskqeesst ssstirkhsk rrqgadafks anaftgayce ydrsyepgac
      181 nfgsifpysn anvfmfmsls ksalsdil
//
LOCUS       XP_077505651             205 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115237 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505651
VERSION     XP_077505651.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649525.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..205
                     /product="uncharacterized protein LOC144115237 isoform X4"
                     /calculated_mol_wt=22411
     CDS             1..205
                     /gene="LOC144115237"
                     /coded_by="XM_077649525.1:1..618"
                     /db_xref="GeneID:144115237"
ORIGIN      
        1 mnaghkalep sgtktdaiep rastskases aariqetrea qslrnatpek gagpakkeps
       61 nltecvpgnl earsgenllp kgqicktlss rimqlhlccq alnlddpkta lptavqvtas
      121 hvytdknqsw iyrgtqaevi alknkkiera iiicpmvyyl knlsyphafs qklnllqkil
      181 spqdqipcgv isnrlkcfla lfkkk
//
LOCUS       XP_077505652             184 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115237 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077505652
VERSION     XP_077505652.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649526.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..184
                     /product="uncharacterized protein LOC144115237 isoform X5"
                     /calculated_mol_wt=19946
     CDS             1..184
                     /gene="LOC144115237"
                     /coded_by="XM_077649526.1:1..555"
                     /db_xref="GeneID:144115237"
ORIGIN      
        1 mnaghkalep sgtktdaiep rastskases aariqetrea qslrnatpek gagpakkgqi
       61 cktlssrimq lhlccqalnl ddpkmmakip pypnvdsfhk avvrnpnysk qeesstssst
      121 irkhskrrqg adafksanaf tgayceydrs yepgacnfgs ifpysnanvf mfmslsksal
      181 sdil
//
LOCUS       XP_077505653             165 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115237 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077505653
VERSION     XP_077505653.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649527.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..165
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..165
                     /product="uncharacterized protein LOC144115237 isoform X6"
                     /calculated_mol_wt=17579
     CDS             1..165
                     /gene="LOC144115237"
                     /coded_by="XM_077649527.1:1..498"
                     /db_xref="GeneID:144115237"
ORIGIN      
        1 mnaghkalep sgtktdaiep rastskases aariqetrea qslrnatpek gagpakkeps
       61 nltecvpgnl earsgenllp kgtqahgghg qicktlssri mqlhlccqal nlddpkitrg
      121 kmpatlanhs hqllvpmkve nlqqlqkemk kssrqlcspp ppppt
//
LOCUS       XP_077505654             157 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115237 isoform X7 [Amblyomma
            americanum].
ACCESSION   XP_077505654
VERSION     XP_077505654.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649528.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..157
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..157
                     /product="uncharacterized protein LOC144115237 isoform X7"
                     /calculated_mol_wt=16833
     CDS             1..157
                     /gene="LOC144115237"
                     /coded_by="XM_077649528.1:1..474"
                     /db_xref="GeneID:144115237"
ORIGIN      
        1 mnaghkalep sgtktdaiep rastskases aariqetrea qslrnatpek gagpakkeps
       61 nltecvpgnl earsgenllp kgqicktlss rimqlhlccq alnlddpkit rgkmpatlan
      121 hshqllvpmk venlqqlqke mkkssrqlcs ppppppt
//
LOCUS       XP_077505655             181 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115238 [Amblyomma americanum].
ACCESSION   XP_077505655
VERSION     XP_077505655.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649529.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..181
                     /product="uncharacterized protein LOC144115238"
                     /calculated_mol_wt=19705
     CDS             1..181
                     /gene="LOC144115238"
                     /coded_by="XM_077649529.1:2174..2719"
                     /db_xref="GeneID:144115238"
ORIGIN      
        1 mystdqegqk lpclyvplgl sflalillsg rlyeaskeia flrvvlvawi pseadlmlgg
       61 aaddhkslvl drsrqtltnl kgdleaflle ltsarttlal svvfvidils gwwmafalkq
      121 akdngsvslr tvlllsvfaa vdiaastgfv plriimvdki irvptkskst grentkeqhc
      181 p
//
LOCUS       XP_077505656             531 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115239 [Amblyomma americanum].
ACCESSION   XP_077505656
VERSION     XP_077505656.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649530.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..531
                     /product="uncharacterized protein LOC144115239"
                     /calculated_mol_wt=60887
     Region          6..81
                     /region_name="THAP"
                     /note="The THAP domain is a putative DNA-binding domain
                     (DBD) and probably also binds a zinc ion; smart00980"
                     /db_xref="CDD:214951"
     Region          <82..>243
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <308..334
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          365..524
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..531
                     /gene="LOC144115239"
                     /coded_by="XM_077649530.1:130..1725"
                     /db_xref="GeneID:144115239"
ORIGIN      
        1 mggftccvpg cynnsardkg lgfyvfpkdh klrqtwlqri nragqtgrfs kfvpttghrv
       61 csahfeggkk tymvrvpsif plrqqkvekr rlltrtepev sststvcteq rgqevsafas
      121 ssnesdcrqp evnilmdhsy ssqelsydel vkkfceqkhi atdlmeqaet anaqvaelkr
      181 qlersrldha etkklcdrlq qknrclrlel ssmqrkvktc kaeaaqnien tyeelvkdek
      241 klryytgfts serleafwsf lepdakrlqf wqmketnsed rkfilplktq lvlvlmrlrl
      301 gldgldlayr fgvskttvsr mwatwldfld nrlcqvptwm ppelcdkyrp kafsdkgytt
      361 vdgildctei fietpssfrv qsetyttykk hntakglvvc spngfvtfvs dlapgrvsdk
      421 altnssgvle kfapgrsvma drgftieeec kqrsldlnip pflegrpqls eedenetrli
      481 asvrihverv irriktyril sqvfpnsmsg qlnkvwhica lltnfvgepl l
//
LOCUS       XP_077505657             545 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115240 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505657
VERSION     XP_077505657.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649531.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..545
                     /product="uncharacterized protein LOC144115240 isoform X1"
                     /calculated_mol_wt=60981
     Region          15..100
                     /region_name="THAP"
                     /note="The THAP domain is a putative DNA-binding domain
                     (DBD) and probably also binds a zinc ion; smart00980"
                     /db_xref="CDD:214951"
     Region          296..347
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          377..537
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..545
                     /gene="LOC144115240"
                     /coded_by="XM_077649531.1:116..1753"
                     /db_xref="GeneID:144115240"
ORIGIN      
        1 maetaqrkqk rgpkyccvvg chnsadnsre rdppvklyrf pgrwyekerr qswitavrrv
       61 npdgtlwqpk ehtricsrhf vgnsksdvsq dpsyiptifp pvyrkkapdq eraqrwqarf
      121 kkeissrqlv gdehpsqsta vgatmqapet slledvhrdf ntmdfsnafl etstatslln
      181 msdshslvlv csarqeshst grkrdaacqt eecatsgkla lllsatngte asaqiahtem
      241 shkvastden wkrncgffgf eslkekeeal qdlcgvtlqv fclllnllpa pqyrrnamsr
      301 edklclflak lrlgitysal gaifgvtatt astvfrktld ilsialknwv fmpsreiikl
      361 slpapfkdny pnctliidct evrtetpsnp dcqhmlyshy kggytlkfli giipngmisf
      421 isrvyggrht dsfitqdsgf lqllkpgdlv lsdkgfpsir ttvegkgavl lmppfnsgvg
      481 qlseqameqt ykiasvrihv erviqrlkiy hvlsnripls lvphmnkvvs vcaalvnmqa
      541 piikk
//
LOCUS       XP_077505658              90 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115240 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505658
VERSION     XP_077505658.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649532.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..90
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..90
                     /product="uncharacterized protein LOC144115240 isoform X2"
                     /calculated_mol_wt=10398
     CDS             1..90
                     /gene="LOC144115240"
                     /coded_by="XM_077649532.1:89..361"
                     /db_xref="GeneID:144115240"
ORIGIN      
        1 mvrerkatil dhrfrmelcg sqknihefaa dilwatarvt sakihptsqp ffhlstekrr
       61 lirkgrkgmp nrgmcyfrea gstslsnkwh
//
LOCUS       XP_077505659            1142 aa            linear   INV 25-NOV-2025
DEFINITION  superkiller complex protein 2-like [Amblyomma americanum].
ACCESSION   XP_077505659
VERSION     XP_077505659.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649533.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1142
                     /product="superkiller complex protein 2-like"
                     /calculated_mol_wt=128060
     Region          251..1134
                     /region_name="Dob10"
                     /note="Superfamily II RNA helicase [Replication,
                     recombination and repair]; COG4581"
                     /db_xref="CDD:443638"
     CDS             1..1142
                     /gene="LOC144115242"
                     /coded_by="XM_077649533.1:380..3808"
                     /db_xref="GeneID:144115242"
ORIGIN      
        1 meslpenlrf kelwggkdvd iepepcsslf dspfpfgipq vipdgrthln silddiwedt
       61 vignlhstqr yfspprnahs llscdvsvap selrilrnpg tgtlegykev ftqgkaledn
      121 kpapdftkdl lhvppgfreg msfgteqpss edkfserhvl nladmlssee qqtlekpsle
      181 akqangnvpg qllddagfle qlpseplstv lnissvvdgq nvclewaeqv diskpmldff
      241 ekvpdpaftw pfeldtfqkk aiahlenres vfvaahtsag ktvvaeyaia lsrrhltrti
      301 ytspikalsn ekyrdfketf sdiglitgdv qinkeascli mtteilrsml ynqskvvadl
      361 ewvifdechy indldrgvvw eevlimlpsh vglvllsatv pnalslanwi grikqrklyv
      421 ictskrpvpl ehhlyfnqet flvldasskf qtasymkaca rrkdtlkitk thddktryqg
      481 liqhlrradr lpaicftfsr rrcdenaqll qsldlttaee kgavrrflqs sviarlsrad
      541 qrlpqlcsmr alleagfgvh hsgvlpilke avemlfqrgl vkvlfatetf amgvnmpart
      601 vifdsirkhd gicvrdllpa eyiqmagrag rrgkdetgtv lllckgdvpe stqlqtmmlg
      661 rptelqsrfr vtysmilnlk aqankrvedm mkdsfrensn qsqsvsmagr casleaelss
      721 lapvecpscq dlclelleee aaarvatweh vlaqpqatrc lspgrlllvr cpeghnllga
      781 lgslaprekr ltlwvladpk pqtsnktplp wplskkiavp sgaltvqqmt imfnsvlciy
      841 aksvkidpkr msqpqcqnvl gqellelvea hpgglpvinv vkdlrlsame tvdlvkrsqq
      901 leekllkskc ltcplfeahf eqehrrrrls eevrhlqhql sedslasmpd yrnhvlaler
      961 lgyvepsgtl tlkgrvarsl sshevmltel llqeslltlg apevaglfsc fvyeqrsnse
     1021 avvprsmkaa vqqfvevalk igrvqresgf eepaeqfleq fsfglcnvvy hwakgmhfah
     1081 imeltevqeg iivrciqrld ellkdvktaa givgnpelra kmgeasrlir rdivfaasly
     1141 lq
//
LOCUS       XP_077505660            1142 aa            linear   INV 25-NOV-2025
DEFINITION  superkiller complex protein 2-like [Amblyomma americanum].
ACCESSION   XP_077505660
VERSION     XP_077505660.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649534.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1142
                     /product="superkiller complex protein 2-like"
                     /calculated_mol_wt=128060
     Region          251..1134
                     /region_name="Dob10"
                     /note="Superfamily II RNA helicase [Replication,
                     recombination and repair]; COG4581"
                     /db_xref="CDD:443638"
     CDS             1..1142
                     /gene="LOC144115242"
                     /coded_by="XM_077649534.1:276..3704"
                     /db_xref="GeneID:144115242"
ORIGIN      
        1 meslpenlrf kelwggkdvd iepepcsslf dspfpfgipq vipdgrthln silddiwedt
       61 vignlhstqr yfspprnahs llscdvsvap selrilrnpg tgtlegykev ftqgkaledn
      121 kpapdftkdl lhvppgfreg msfgteqpss edkfserhvl nladmlssee qqtlekpsle
      181 akqangnvpg qllddagfle qlpseplstv lnissvvdgq nvclewaeqv diskpmldff
      241 ekvpdpaftw pfeldtfqkk aiahlenres vfvaahtsag ktvvaeyaia lsrrhltrti
      301 ytspikalsn ekyrdfketf sdiglitgdv qinkeascli mtteilrsml ynqskvvadl
      361 ewvifdechy indldrgvvw eevlimlpsh vglvllsatv pnalslanwi grikqrklyv
      421 ictskrpvpl ehhlyfnqet flvldasskf qtasymkaca rrkdtlkitk thddktryqg
      481 liqhlrradr lpaicftfsr rrcdenaqll qsldlttaee kgavrrflqs sviarlsrad
      541 qrlpqlcsmr alleagfgvh hsgvlpilke avemlfqrgl vkvlfatetf amgvnmpart
      601 vifdsirkhd gicvrdllpa eyiqmagrag rrgkdetgtv lllckgdvpe stqlqtmmlg
      661 rptelqsrfr vtysmilnlk aqankrvedm mkdsfrensn qsqsvsmagr casleaelss
      721 lapvecpscq dlclelleee aaarvatweh vlaqpqatrc lspgrlllvr cpeghnllga
      781 lgslaprekr ltlwvladpk pqtsnktplp wplskkiavp sgaltvqqmt imfnsvlciy
      841 aksvkidpkr msqpqcqnvl gqellelvea hpgglpvinv vkdlrlsame tvdlvkrsqq
      901 leekllkskc ltcplfeahf eqehrrrrls eevrhlqhql sedslasmpd yrnhvlaler
      961 lgyvepsgtl tlkgrvarsl sshevmltel llqeslltlg apevaglfsc fvyeqrsnse
     1021 avvprsmkaa vqqfvevalk igrvqresgf eepaeqfleq fsfglcnvvy hwakgmhfah
     1081 imeltevqeg iivrciqrld ellkdvktaa givgnpelra kmgeasrlir rdivfaasly
     1141 lq
//
LOCUS       XP_077505663             942 aa            linear   INV 25-NOV-2025
DEFINITION  serine/threonine-protein phosphatase 1 regulatory subunit 10-like
            [Amblyomma americanum].
ACCESSION   XP_077505663
VERSION     XP_077505663.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649537.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..942
                     /product="serine/threonine-protein phosphatase 1
                     regulatory subunit 10-like"
                     /calculated_mol_wt=101537
     Region          77..141
                     /region_name="TFIIS_I"
                     /note="N-terminal domain (domain I) of transcription
                     elongation factor S-II (TFIIS); similar to a domain found
                     in elongin A and CRSP70; likely to be involved in
                     transcription; domain I from TFIIS interacts with RNA
                     polymerase II holoenzyme; cl00146"
                     /db_xref="CDD:469629"
     Region          <421..644
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          914..934
                     /region_name="ZnF_C3H1"
                     /note="zinc finger; smart00356"
                     /db_xref="CDD:214632"
     CDS             1..942
                     /gene="LOC144115244"
                     /coded_by="XM_077649537.1:420..3248"
                     /db_xref="GeneID:144115244"
ORIGIN      
        1 mapidplqll kalspllspe ggirssdevs rlsslmkkfs rklvsrciyc nvlratspdi
       61 levfldldgw stihmwlqea ktaenmpflc ellelcqilp mslerlkens spklikslmk
      121 stdenvrlta ekvvngwmkl ikgdtkeaik pdkdadatvk kkkkssstns tkaaagkrdr
      181 skgsaddhis ennsnatsnt hgpskssssp tasssdkesp ptlkettpkk pkladrpktv
      241 ktlktrfrst gleeaplppp pktgtkkvsr pafdktavls arqrlaptvh terrvkpssl
      301 vsmgvegrdt agpgirliap kpvhvlhedg gfmdaltaap ppssappvrr krkpsgaata
      361 agaggakgaa atsptsptpa apkfqfykdt letteaagee esekpkevkv eeedededig
      421 krvkrrkrgq qatvvegkeg dniespered spptptatgg deevttgegq evgskssegd
      481 dkevsvkked egeekssegd ttsekpasil smtrkktkks vrwadesklk qfhyfeldet
      541 ervnvnnlhn fgdmktlemk rerqavetar rlmgdrmeea ipfrtprrlt lpeplaepga
      601 nsreketqkl rqqktlqeiy fskemipdsp yeadpeqlft qepkiipled enspggtldy
      661 shmelpktss lppilsnlvl siatkgqqpq gggsphqgvq qqqqqqlhqq qqqqqqqqlq
      721 ggllpgqfpg ggpvqqqgal lhtlpplhhg gagtqivadm qqqqamsmmg lmapdggqsg
      781 mhiqpgpaga vpigvdpngp pfmhpnsmpn qgigmmgppm pmggaggfpm ggggpmhphg
      841 vgsnagdwgm mhpgipqdga dyygrghhpg mgppmgmpgp pmgagggphm mdmprggpph
      901 grssrmrnhp pripckyyms sqcrfgagcs flhpgvngpp lq
//
LOCUS       XP_077505664             219 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115245 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505664
VERSION     XP_077505664.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649538.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..219
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..219
                     /product="uncharacterized protein LOC144115245 isoform X1"
                     /calculated_mol_wt=25002
     CDS             1..219
                     /gene="LOC144115245"
                     /coded_by="XM_077649538.1:141..800"
                     /db_xref="GeneID:144115245"
ORIGIN      
        1 maalmwracl rrslraackp lpvylqsvek klllpetski qelssvcsls rlegckklst
       61 sacsfckeee eveteggaed seetsddgtl nvkkvtlhpp elsiqylesk ayketygddp
      121 vwtmyrrnfk gqfppkktrr ncvntklltq fisthtgetl kpnktglcrr kqfeldlail
      181 kakdlgliky qvpfrrydyn dyypqlkest kekvaslpe
//
LOCUS       XP_077505665             243 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115245 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505665
VERSION     XP_077505665.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649539.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..243
                     /product="uncharacterized protein LOC144115245 isoform X2"
                     /calculated_mol_wt=27667
     Region          154..214
                     /region_name="Ribosomal_S18"
                     /note="Ribosomal protein S18; cl46633"
                     /db_xref="CDD:480973"
     CDS             1..243
                     /gene="LOC144115245"
                     /coded_by="XM_077649539.1:160..891"
                     /db_xref="GeneID:144115245"
ORIGIN      
        1 maalmwracl rrslraackp lpvylqsvek klllpetski qelssvcsls rlegckklst
       61 sacsfckeee eveteggaed seetsddgtl nvkkvtlhpp elsiqylesk ayketygddp
      121 vwtmyrrnfk gqfppkktrr ncvkkgmiat gnpcplcrde ylvvhhtntk lltqfistht
      181 getlkpnktg lcrrkqfeld lailkakdlg likyqvpfrr ydyndyypql kestkekvas
      241 lpe
//
LOCUS       XP_077505666             882 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505666
VERSION     XP_077505666.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649540.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..882
                     /product="nose resistant to fluoxetine protein 6-like
                     isoform X1"
                     /calculated_mol_wt=96726
     Region          <27..>176
                     /region_name="PRK07003"
                     /note="DNA polymerase III subunit gamma/tau"
                     /db_xref="CDD:235906"
     Region          211..331
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          420..825
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..882
                     /gene="LOC144115246"
                     /coded_by="XM_077649540.1:116..2764"
                     /db_xref="GeneID:144115246"
ORIGIN      
        1 marlrnaepl lrllvplill lrpispaeaa arkdsgaaad gplafrkdss glqapdtlag
       61 sfpdpalnaa lygdaatvds sllkasqapd kpedvaaita pgensagtlp qsqspgqnle
      121 aevespplht aaealvqslv etaasssasp peatdaeqee epgeptttaa appgsgfdle
      181 snlravieri fkealplvyr fstgagsspe cmgalfkwvl airrlepwal rmvdamgrpp
      241 agifegtitd fgsfdqclet raqdswgdes frgqycslfl kpnidtsrpv sflrnsrrnl
      301 srimksiwfp gfrlgicvps acsrndidvl iksafknygv nasvplcdve rdiqldnfqq
      361 aaliclcvvl smvvlgtlvd vflrsrqdkd rkphavlgal tswsaysntl rlfdvsddgs
      421 rlralhgvrf ltmlwiilgy cyaltpipnr rrifnmfdfv khtpfmfian aypaadtffc
      481 lsgflfgysv fkqrrnlskw lpillirryv rvivpcfcll lvfsllglvs sgpiwrdnya
      541 llrgncearw wkipalvnnw eysldsclph lwfyaadlql lvgftpavvl lvrwpkagla
      601 ltlamvlacs vyialqtlys dlypvtiyfa edvmksrrth tevfkrpfsh agafslglll
      661 aymlrsrtpr edgaakgsgh rcgrgawraa gwaaasgcgl avllgveqwh kgllpgpava
      721 alyaslhkta wgllvawvay vvctdrkgip srflswgpfv alsrlsfsay llhilvlfvr
      781 fgsirerlys shfiqmcefi vvtvlsysga yvfhllfeap stqfltmats avagrlllae
      841 eaparrltts nqvvhiavne kppkeangse gvhgqgprst hl
//
LOCUS       XP_077505667             780 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505667
VERSION     XP_077505667.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649541.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..780
                     /product="nose resistant to fluoxetine protein 6-like
                     isoform X2"
                     /calculated_mol_wt=85275
     Region          <27..>176
                     /region_name="PRK07003"
                     /note="DNA polymerase III subunit gamma/tau"
                     /db_xref="CDD:235906"
     Region          318..723
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..780
                     /gene="LOC144115246"
                     /coded_by="XM_077649541.1:92..2434"
                     /db_xref="GeneID:144115246"
ORIGIN      
        1 marlrnaepl lrllvplill lrpispaeaa arkdsgaaad gplafrkdss glqapdtlag
       61 sfpdpalnaa lygdaatvds sllkasqapd kpedvaaita pgensagtlp qsqspgqnle
      121 aevespplht aaealvqslv etaasssasp peatdaeqee epgeptttaa appgsgfdle
      181 snlravieri fkealplvyr fstgagsspe cmgalfkwvl airrlepwal rtfknygvna
      241 svplcdverd iqldnfqqaa liclcvvlsm vvlgtlvdvf lrsrqdkdrk phavlgalts
      301 wsaysntlrl fdvsddgsrl ralhgvrflt mlwiilgycy altpipnrrr ifnmfdfvkh
      361 tpfmfianay paadtffcls gflfgysvfk qrrnlskwlp illirryvrv ivpcfclllv
      421 fsllglvssg piwrdnyall rgncearwwk ipalvnnwey sldsclphlw fyaadlqllv
      481 gftpavvllv rwpkaglalt lamvlacsvy ialqtlysdl ypvtiyfaed vmksrrthte
      541 vfkrpfshag afslglllay mlrsrtpred gaakgsghrc grgawraagw aaasgcglav
      601 llgveqwhkg llpgpavaal yaslhktawg llvawvayvv ctdrkgipsr flswgpfval
      661 srlsfsayll hilvlfvrfg sirerlyssh fiqmcefivv tvlsysgayv fhllfeapst
      721 qfltmatsav agrlllaeea parrlttsnq vvhiavnekp pkeangsegv hgqgprsthl
//
LOCUS       XP_077505668             830 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115248 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505668
VERSION     XP_077505668.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649542.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..830
                     /product="uncharacterized protein LOC144115248 isoform X1"
                     /calculated_mol_wt=89226
     CDS             1..830
                     /gene="LOC144115248"
                     /coded_by="XM_077649542.1:282..2774"
                     /db_xref="GeneID:144115248"
ORIGIN      
        1 mgtcysskgk saslpyssap msasssqdsa lrlprptpyt rgsvapidas evtfkrcipp
       61 ptkyrtnhap pgnakqshpk tgvtvttdia ergrkavtdn advnnpslnn nfvegnpsrd
      121 itnfkgkild satldvvdng apelktegak lslensdqlr iqygttkipt kitahlsglp
      181 kpqvavvttr ippvpqraas eklpaatral qptgeagaae krtvcqnrvs pdkesisdrs
      241 hsgdsgstdc dsglghsvsd ppagcesggk aqsssmtlqe espavlkeat gagkvldsav
      301 lpasfpvgaq latadgaask mgvydqpdsy aayrgilsyr ritpikkyse apqwkrtvep
      361 knskglgsps raviakgilt kgaraahatk dssleqkret gakvvstlps apltptkeek
      421 nggflsptsd gflidddisd qpglialers rgrgapsvkk sifelhalqs fsgrqnkidr
      481 tlddlhplcs dagsscsslg sddlmldfet sckdpldvas sptvkytgtp lrrlarssia
      541 shaekrnswd snhsdegaal rrasdslasr wplktpcsaa apsegrprti slplrpprqv
      601 vmqecddggv kldsssyrsv cqdlnglktm lfklrrilqd aetlnpfels ntrnffyhal
      661 astdlphgft ntptgetpdy npidpseivq enmdlkrqli lmqqqledkd htirllqqqm
      721 tkymnivste kdpgkvnaav qterpkflqs isssseevss rgrlvssrmk ealeehlkss
      781 sichaafrdk iddlskslcn itqalrqhgs taqlpsrsqs swellshgdt
//
LOCUS       XP_077505669             820 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115248 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505669
VERSION     XP_077505669.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649543.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..820
                     /product="uncharacterized protein LOC144115248 isoform X2"
                     /calculated_mol_wt=88249
     CDS             1..820
                     /gene="LOC144115248"
                     /coded_by="XM_077649543.1:282..2744"
                     /db_xref="GeneID:144115248"
ORIGIN      
        1 mgtcysskgk saslpyssap msasssqdsa lrlprptpyt rgsvapidas evtfkrcipp
       61 ptkyrtnhap pgnakqshpk tgvtvttdia ergrkavtdn advnnpslnn nfvegnpsrd
      121 itnfkgkild satldvvdng apelktegak lslensdqlr iqygttkipt kitahlsglp
      181 kpqvavvttr ippvpqraas eklpaatral qptgeagaae krtvcqnrvs pdkesisdrs
      241 hsgdsgstdc dsglghsvsd ppagcesggk aqsssmtlqe espavlkeat gagkvldsav
      301 lpasfpvgaq latadgaask mgvydqpdsy aayrgilsyr ritpikkyse apqwkrtvep
      361 knskglgsps raviakgilt kgaraahatk dssleqkret gakvvstlps apltptkeek
      421 nggflsptsd gflidddisd qpglialers rgrgapsvkk sifelhalqs fsgrqnkidr
      481 tlddlhplcs dagsscsslg sddlmldfet sckdpldvas sptvkytgtp lrrlarssia
      541 shaekrnswd snhsdegaal rrasdslasr wplktpcsaa apsegrprti slplrpprqv
      601 vmqecddggv kldsssyrsv cqdlnglktm lfklrrilqd aetlnpfels ntrnffyhal
      661 astdlphgft ntptgetpdy npidpseivq enmdlkrqli lmqqqledkd htirllqqqm
      721 tkymnivste kdpgkvnaav qterpkflqs isssseevss rgrlvrfrqs ggstvtrkqr
      781 iwecadypgv dedidsresw eallrseepa rqkqgnppgg
//
LOCUS       XP_077505670             777 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115248 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505670
VERSION     XP_077505670.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649544.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..777
                     /product="uncharacterized protein LOC144115248 isoform X3"
                     /calculated_mol_wt=83484
     CDS             1..777
                     /gene="LOC144115248"
                     /coded_by="XM_077649544.1:282..2615"
                     /db_xref="GeneID:144115248"
ORIGIN      
        1 mgtcysskgk saslpyssap msasssqdsa lrlprptpyt rgsvapidas evtfkrcipp
       61 ptkyrtnhap pgnakqshpk tgvtvttdia ergrkavtdn advnnpslnn nfvegnpsrd
      121 itnfkgkild satldvvdng apelktegak lslensdqlr iqygttkipt kitahlsglp
      181 kpqvavvttr ippvpqraas eklpaatral qptgeagaae krtvcqnrvs pdkesisdrs
      241 hsgdsgstdc dsglghsvsd ppagcesggk aqsssmtlqe espavlkeat gagkvldsav
      301 lpasfpvgaq latadgaask mgvydqpdsy aayrgilsyr ritpikkyse apqwkrtvep
      361 knskglgsps raviakgilt kgaraahatk dssleqkret gakvvstlps apltptkeek
      421 nggflsptsd gflidddisd qpglialers rgrgapsvkk sifelhalqs fsgrqnkidr
      481 tlddlhplcs dagsscsslg sddlmldfet sckdpldvas sptvkytgtp lrrlarssia
      541 shaekrnswd snhsdegaal rrasdslasr wplktpcsaa apsegrprti slplrpprqv
      601 vmqecddggv kldsssyrsv cqdlnglktm lfklrrilqd aetlnpfels ntrnffyhal
      661 astdlphgft ntptgetpdy npidpseivq enmdlkrqli lmqqqledkd htirllqqqm
      721 tkymnivste kdpgkvnaav qterpkflqs isssseevss rgrlvsfrig qlhtnyf
//
LOCUS       XP_077505671             772 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115248 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505671
VERSION     XP_077505671.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649545.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..772
                     /product="uncharacterized protein LOC144115248 isoform X4"
                     /calculated_mol_wt=82855
     CDS             1..772
                     /gene="LOC144115248"
                     /coded_by="XM_077649545.1:282..2600"
                     /db_xref="GeneID:144115248"
ORIGIN      
        1 mgtcysskgk saslpyssap msasssqdsa lrlprptpyt rgsvapidas evtfkrcipp
       61 ptkyrtnhap pgnakqshpk tgvtvttdia ergrkavtdn advnnpslnn nfvegnpsrd
      121 itnfkgkild satldvvdng apelktegak lslensdqlr iqygttkipt kitahlsglp
      181 kpqvavvttr ippvpqraas eklpaatral qptgeagaae krtvcqnrvs pdkesisdrs
      241 hsgdsgstdc dsglghsvsd ppagcesggk aqsssmtlqe espavlkeat gagkvldsav
      301 lpasfpvgaq latadgaask mgvydqpdsy aayrgilsyr ritpikkyse apqwkrtvep
      361 knskglgsps raviakgilt kgaraahatk dssleqkret gakvvstlps apltptkeek
      421 nggflsptsd gflidddisd qpglialers rgrgapsvkk sifelhalqs fsgrqnkidr
      481 tlddlhplcs dagsscsslg sddlmldfet sckdpldvas sptvkytgtp lrrlarssia
      541 shaekrnswd snhsdegaal rrasdslasr wplktpcsaa apsegrprti slplrpprqv
      601 vmqecddggv kldsssyrsv cqdlnglktm lfklrrilqd aetlnpfels ntrnffyhal
      661 astdlphgft ntptgetpdy npidpseivq enmdlkrqli lmqqqledkd htirllqqqm
      721 tkymnivste kdpgkvnaav qterpkflqs isssseevss rgrlvsfrig qf
//
LOCUS       XP_077505672             772 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115248 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077505672
VERSION     XP_077505672.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649546.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..772
                     /product="uncharacterized protein LOC144115248 isoform X5"
                     /calculated_mol_wt=83245
     CDS             1..772
                     /gene="LOC144115248"
                     /coded_by="XM_077649546.1:282..2600"
                     /db_xref="GeneID:144115248"
ORIGIN      
        1 mgtcysskgk saslpyssap msasssqdsa lrlprptpyt rgsvapidas evtfkrcipp
       61 ptkyrtnhap pgnakqshpk tgvtvttdia ergrkavtdn advnnpslnn nfvegnpsrd
      121 itnfkgkild satldvvdng apelktegak lslensdqlr iqygttkipt kitahlsglp
      181 kpqvavvttr ippvpqraas eklpaatral qptgeagaae krtvcqnrvs pdkesisdrs
      241 hsgdsgstdc dsglghsvsd ppagcesggk aqsssmtlqe espavlkeat gagkvldsav
      301 lpasfpvgaq latadgaask mgvydqpdsy aayrgilsyr ritpikkyse apqwkrtvep
      361 knskglgsps raviakgilt kgaraahatk dssleqkret gakvvstlps apltptkeek
      421 nggflsptsd gflidddisd qpglialers rgrgapsvkk sifelhalqs fsgrqnkidr
      481 tlddlhplcs dagsscsslg sddlmldfet sckdpldvas sptvkytgtp lrrlarssia
      541 shaekrnswd snhsdegaal rrasdslasr wplktpcsaa apsegrprti slplrpprqv
      601 vmqecddggv kldsssyrsv cqdlnglktm lfklrrilqd aetlnpfels ntrnffyhal
      661 astdlphgft ntptgetpdy npidpseivq enmdlkrqli lmqqqledkd htirllqqqm
      721 tkymnivste kdpgkvnaav qtervptvrr fhsypeaenm gmrrlprsrr ry
//
LOCUS       XP_077505673             745 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115248 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077505673
VERSION     XP_077505673.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649547.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..745
                     /product="uncharacterized protein LOC144115248 isoform X6"
                     /calculated_mol_wt=79891
     CDS             1..745
                     /gene="LOC144115248"
                     /coded_by="XM_077649547.1:292..2529"
                     /db_xref="GeneID:144115248"
ORIGIN      
        1 mgtcysskgk saslpyssap msasssqdsa lrlprptpyt rgsvapidas evtfkrcipp
       61 ptkyrtnhap pgnakqshpk tgvtvttdia ergrkavtdn advnnpslnn nfvegnpsrd
      121 itnfkgkild satldvvdng apelktegak lslensdqlr iqygttkipt kitahlsglp
      181 kpqvavvttr ippvpqraas eklpaatral qptgeagaae krtvcqnrvs pdkesisdrs
      241 hsgdsgstdc dsglghsvsd ppagcesggk aqsssmtlqe espavlkeat gagkvldsav
      301 lpasfpvgaq latadgaask mgvydqpdsy aayrgilsyr ritpikkyse apqwkrtvep
      361 knskglgsps raviakgilt kgaraahatk dssleqkret gakvvstlps apltptkeek
      421 nggflsptsd gflidddisd qpglialers rgrgapsvkk sifelhalqs fsgrqnkidr
      481 tlddlhplcs dagsscsslg sddlmldfet sckdpldvas sptvkytgtp lrrlarssia
      541 shaekrnswd snhsdegaal rrasdslasr wplktpcsaa apsegrprti slplrpprqv
      601 vmqecddggv kldsssyrsv cqdlnglktm lfklrrilqd aetlnpfels ntrnffyhal
      661 astdlphgft ntptgetpdy npidpseivq enmdlkrqli lmqqqledkd htirllqqqm
      721 tkymnivste kdpgkvnaav qterf
//
LOCUS       XP_077505674             420 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115250 [Amblyomma americanum].
ACCESSION   XP_077505674
VERSION     XP_077505674.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649548.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 12% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..420
                     /product="uncharacterized protein LOC144115250"
                     /calculated_mol_wt=47407
     Region          74..>110
                     /region_name="FH_FOX"
                     /note="Forkhead (FH) domain found in Forkhead box (FOX)
                     family of transcription factors and similar proteins;
                     cl00061"
                     /db_xref="CDD:469596"
     CDS             1..420
                     /gene="LOC144115250"
                     /coded_by="XM_077649548.1:1..1263"
                     /db_xref="GeneID:144115250"
ORIGIN      
        1 mntkrrnrni pskrrrhvsa katgktarqa fpphphhvqp rrhvhhapat sgspgraapc
       61 whhrpqasgq gklvqlvaqa ihespikalr vrhvyealkk rypyfmfmdk kelttwegse
      121 srpssrlsss sqsllvhtpl psfigspgrq lghmepqlhp lvewelkter fatppspsiy
      181 lnteqerkpk pvyswsrgcy dlyghqsqgy sdevstaadt qllneplpsl pylcsprlth
      241 dtpveptsrs yghpsphqep qlpltleqhs rpelsfarqq iqpeqeammp qqqflprpel
      301 snsregtlph pelplppqfp wppqgpslpr gptlpprqee trpwgtgtgf wprsgrlqlp
      361 gwpyagepvf aaianlgrav mmddgtwyil vypafpende csaravwhwe eritaggnal
//
LOCUS       XP_077505675             168 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115251 [Amblyomma americanum].
ACCESSION   XP_077505675
VERSION     XP_077505675.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649549.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..168
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..168
                     /product="uncharacterized protein LOC144115251"
                     /calculated_mol_wt=18904
     Region          5..85
                     /region_name="THAP"
                     /note="The THAP domain is a putative DNA-binding domain
                     (DBD) and probably also binds a zinc ion; smart00980"
                     /db_xref="CDD:214951"
     CDS             1..168
                     /gene="LOC144115251"
                     /coded_by="XM_077649549.1:63..569"
                     /db_xref="GeneID:144115251"
ORIGIN      
        1 mpttcvaygc naryvkgskl gffrfpnasr drlrreawik avrrlddrgr hwapssasrl
       61 cgnhfetgrp rmsprhrdfv ptlfsfsckk akrasavsrf qramertkkk klreqgstps
      121 gtlvsavqgg gdddmvidgd segdlhgtqs qqssdlccsw rffkqrpk
//
LOCUS       XP_077505676             411 aa            linear   INV 25-NOV-2025
DEFINITION  heterogeneous nuclear ribonucleoprotein F-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077505676
VERSION     XP_077505676.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649550.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..411
                     /product="heterogeneous nuclear ribonucleoprotein F-like
                     isoform X1"
                     /calculated_mol_wt=44310
     Region          11..89
                     /region_name="RRM1_hnRNPH_GRSF1_like"
                     /note="RNA recognition motif 1 (RRM1) found in
                     heterogeneous nuclear ribonucleoprotein (hnRNP) H protein
                     family, G-rich sequence factor 1 (GRSF-1) and similar
                     proteins; cd12503"
                     /db_xref="CDD:409926"
     Region          94..188
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          263..337
                     /region_name="RRM3_hnRNPH_CRSF1_like"
                     /note="RNA recognition motif 3 (RRM3) found in
                     heterogeneous nuclear ribonucleoprotein hnRNP H protein
                     family, G-rich sequence factor 1 (GRSF-1) and similar
                     proteins; cd12506"
                     /db_xref="CDD:409929"
     CDS             1..411
                     /gene="LOC144115252"
                     /coded_by="XM_077649550.1:146..1381"
                     /db_xref="GeneID:144115252"
ORIGIN      
        1 maapvddegf vlrirglpws ttkeeilnff tskechikgg ingvhmtlsr egrpsgeayi
       61 eleseqdvei glqrhnehig hryievfksk rsemdwvvkr sgahqqdsln dgcvrlrglp
      121 fgcskeeiaq ffsgleivpn gitlptdyqg rstgeafvqf atrdiaekam gkhkekighr
      181 yieifksslq eiraavgmgm pkmmrpmgta rpgpydradr fgggpsrygi grgsrnfrgf
      241 veedgygefg gsgggarfsa tghfvhmrgl pfraterdif effqpmnpvn vhliyedsgr
      301 psgecdvefg theeavkams kdkahmqhry ielflnstps glnagggnag gfgngvgpig
      361 sasgtpafag tmssggsgfg sssfgsgrfg ggsyggsmgg nmmggnnyts f
//
LOCUS       XP_077505677             441 aa            linear   INV 25-NOV-2025
DEFINITION  heterogeneous nuclear ribonucleoprotein F-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077505677
VERSION     XP_077505677.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649551.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..441
                     /product="heterogeneous nuclear ribonucleoprotein F-like
                     isoform X2"
                     /calculated_mol_wt=47663
     Region          41..119
                     /region_name="RRM1_hnRNPH_GRSF1_like"
                     /note="RNA recognition motif 1 (RRM1) found in
                     heterogeneous nuclear ribonucleoprotein (hnRNP) H protein
                     family, G-rich sequence factor 1 (GRSF-1) and similar
                     proteins; cd12503"
                     /db_xref="CDD:409926"
     Region          124..218
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          293..367
                     /region_name="RRM3_hnRNPH_CRSF1_like"
                     /note="RNA recognition motif 3 (RRM3) found in
                     heterogeneous nuclear ribonucleoprotein hnRNP H protein
                     family, G-rich sequence factor 1 (GRSF-1) and similar
                     proteins; cd12506"
                     /db_xref="CDD:409929"
     CDS             1..441
                     /gene="LOC144115252"
                     /coded_by="XM_077649551.1:67..1392"
                     /db_xref="GeneID:144115252"
ORIGIN      
        1 mtrltvsayq svlrllrtls csvvpmdtdn maapvddegf vlrirglpws ttkeeilnff
       61 tskechikgg ingvhmtlsr egrpsgeayi eleseqdvei glqrhnehig hryievfksk
      121 rsemdwvvkr sgahqqdsln dgcvrlrglp fgcskeeiaq ffsgleivpn gitlptdyqg
      181 rstgeafvqf atrdiaekam gkhkekighr yieifksslq eiraavgmgm pkmmrpmgta
      241 rpgpydradr fgggpsrygi grgsrnfrgf veedgygefg gsgggarfsa tghfvhmrgl
      301 pfraterdif effqpmnpvn vhliyedsgr psgecdvefg theeavkams kdkahmqhry
      361 ielflnstps glnagggnag gfgngvgpig sasgtpafag tmssggsgfg sssfgsgrfg
      421 ggsyggsmgg nmmggnnyts f
//
LOCUS       XP_077505678             612 aa            linear   INV 25-NOV-2025
DEFINITION  3'-phosphoadenosine 5'-phosphosulfate synthase isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077505678
VERSION     XP_077505678.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649552.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..612
                     /product="3'-phosphoadenosine 5'-phosphosulfate synthase
                     isoform X1"
                     /calculated_mol_wt=68529
     Region          22..216
                     /region_name="CysC"
                     /note="Adenylylsulfate kinase or related kinase [Inorganic
                     ion transport and metabolism]; COG0529"
                     /db_xref="CDD:440295"
     Region          243..606
                     /region_name="ATPS"
                     /note="ATP-sulfurylase; cd00517"
                     /db_xref="CDD:173895"
     Site            order(407..411,417,420,484,509..511,513..514,550..552)
                     /site_type="active"
                     /db_xref="CDD:173895"
     Site            414..417
                     /site_type="other"
                     /note="HXXH motif"
                     /db_xref="CDD:173895"
     Site            445..455
                     /site_type="other"
                     /note="flexible loop"
                     /db_xref="CDD:173895"
     CDS             1..612
                     /gene="Papss"
                     /coded_by="XM_077649552.1:237..2075"
                     /db_xref="GeneID:144115255"
ORIGIN      
        1 mpniscnfet nhkstnvvfq khhvsrdkrg qimgqkggfr gctiwftgls gagkttvsfg
       61 veeylclhgi payaldgdni rhglnknlgf ssqdreenir rvaevaklfa dsgvvcltsf
      121 ispyakdrtm areiheqsgl lfiecfvdtp levcekrdvk glyrkaragq ikgftgidmp
      181 yeapdtpdll ikagkdtikd cvqqvvellr ergvipgsvv dcvkelfvsp erlpammaea
      241 aglpsveisk vdlqwvqvls egwatpltgf mreaeflqsq hfgcyleggv tnqsipivlp
      301 vttedmkrle nepafalkhn gkvyavlrqp efyphrkeer csrqfgtscr ghpyinmiye
      361 sgdwlvggdl evlerirwdd gldeyrltpk elrkafsklg adavfafqlr npvhnghall
      421 mqdtrkhlte kgykkpvlll hplggwtkdd dvplavrmkq hkavldsgvl dpkmtvlaif
      481 pspmmyagpt evqwhakarm vcgsnfyivg rdpagmphpe tkqdlydpth gakvltmapg
      541 ltqleiipfq vaaydtkkkk malfeperke dfqfisgtkm rslarsgqep pagfmepsaw
      601 kvladyyrsv tn
//
LOCUS       XP_077505679             612 aa            linear   INV 25-NOV-2025
DEFINITION  3'-phosphoadenosine 5'-phosphosulfate synthase isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077505679
VERSION     XP_077505679.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649553.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..612
                     /product="3'-phosphoadenosine 5'-phosphosulfate synthase
                     isoform X2"
                     /calculated_mol_wt=68618
     Region          22..216
                     /region_name="CysC"
                     /note="Adenylylsulfate kinase or related kinase [Inorganic
                     ion transport and metabolism]; COG0529"
                     /db_xref="CDD:440295"
     Region          243..606
                     /region_name="ATPS"
                     /note="ATP-sulfurylase; cd00517"
                     /db_xref="CDD:173895"
     Site            order(407..411,417,420,484,509..511,513..514,550..552)
                     /site_type="active"
                     /db_xref="CDD:173895"
     Site            414..417
                     /site_type="other"
                     /note="HXXH motif"
                     /db_xref="CDD:173895"
     Site            445..455
                     /site_type="other"
                     /note="flexible loop"
                     /db_xref="CDD:173895"
     CDS             1..612
                     /gene="Papss"
                     /coded_by="XM_077649553.1:452..2290"
                     /db_xref="GeneID:144115255"
ORIGIN      
        1 mincvafqyr nhkstnvvfq khhvsrdkrg qimgqkggfr gctiwftgls gagkttvsfg
       61 veeylclhgi payaldgdni rhglnknlgf ssqdreenir rvaevaklfa dsgvvcltsf
      121 ispyakdrtm areiheqsgl lfiecfvdtp levcekrdvk glyrkaragq ikgftgidmp
      181 yeapdtpdll ikagkdtikd cvqqvvellr ergvipgsvv dcvkelfvsp erlpammaea
      241 aglpsveisk vdlqwvqvls egwatpltgf mreaeflqsq hfgcyleggv tnqsipivlp
      301 vttedmkrle nepafalkhn gkvyavlrqp efyphrkeer csrqfgtscr ghpyinmiye
      361 sgdwlvggdl evlerirwdd gldeyrltpk elrkafsklg adavfafqlr npvhnghall
      421 mqdtrkhlte kgykkpvlll hplggwtkdd dvplavrmkq hkavldsgvl dpkmtvlaif
      481 pspmmyagpt evqwhakarm vcgsnfyivg rdpagmphpe tkqdlydpth gakvltmapg
      541 ltqleiipfq vaaydtkkkk malfeperke dfqfisgtkm rslarsgqep pagfmepsaw
      601 kvladyyrsv tn
//
LOCUS       XP_077505681             555 aa            linear   INV 25-NOV-2025
DEFINITION  glutamate dehydrogenase, mitochondrial [Amblyomma americanum].
ACCESSION   XP_077505681
VERSION     XP_077505681.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649555.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..555
                     /product="glutamate dehydrogenase, mitochondrial"
                     /calculated_mol_wt=61717
     Region          111..552
                     /region_name="GdhA"
                     /note="Glutamate dehydrogenase/leucine dehydrogenase
                     [Amino acid transport and metabolism]; COG0334"
                     /db_xref="CDD:440103"
     CDS             1..555
                     /gene="Gdh"
                     /coded_by="XM_077649555.1:196..1863"
                     /db_xref="GeneID:144115256"
ORIGIN      
        1 mlrygavtlg qlasvygkag icrkavksai apmvsqhqrr sysdhkmpeq lqyvedaedp
       61 sffhmveyfy hrgwqvvedk lveefkgkls leekrkkvrg ylallgpcha vlevsfplkr
      121 dngeyvming wraqhshhrt pckggirysl dvsldevkal salmtfkcav vdvpfgggka
      181 glkinprefs efelekitrn falqlakkgf lgpgvdvpap dmgtgerems wiadtysqti
      241 ghsdlnshac itgkpinqgg ihgrvsatgr gvfhgidnfi neasfmsmig ttpgwggktf
      301 ivqglgnvgl hctryltrag arcigiqevd gsifnpngid pkeieewkin ngtingfpga
      361 epykgenliy eecdifvpaa lekiitkdna nkfkckiiae aangpttpaa dkilmernvl
      421 vipdlyinag gvtvsyfewl knlnhvsygr llfkyeresn yhllesvqes lerrfgkagg
      481 ripimpsesf qkrisgasek divhsgldyt mersarqimr tamkynlgld lrtaayvnsi
      541 ekiyltyrea gltft
//
LOCUS       XP_077505682             371 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial ribosomal protein L1 [Amblyomma americanum].
ACCESSION   XP_077505682
VERSION     XP_077505682.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649556.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..371
                     /product="mitochondrial ribosomal protein L1"
                     /calculated_mol_wt=41731
     Region          129..339
                     /region_name="Ribosomal_L1"
                     /note="Ribosomal protein L1. The L1 protein, located near
                     the E-site of the ribosome, forms part of the L1 stalk
                     along with 23S rRNA. In bacteria and archaea, L1 functions
                     both as a ribosomal protein that binds rRNA, and as a
                     translation repressor that binds...; cd00403"
                     /db_xref="CDD:238235"
     CDS             1..371
                     /gene="mRpL1"
                     /coded_by="XM_077649556.1:49..1164"
                     /db_xref="GeneID:144115257"
ORIGIN      
        1 mrwhdlrgpv lvfgnmaalv asraclwlsp svrtvvrtha pcvafaaget lqirsyasqk
       61 hqklilaaqk rnrqrklekp pekdklawik ksknlligpr retyedrekd ppvddvyftd
      121 kymptvypaa eaiamhreth hptvlcdpda llyafielnl stkkktkfre dfsgtvlysh
      181 pftvtkrekr iialckntdv atealaagae vaggtviikr ilageldtky fdyilahvdt
      241 mqdlpqlrgl lktkfpsaan grvtndlpsl vqgyfkgidf ssksdpheld ygfvevpvge
      301 lsmptehltd niktllanid afkpkgtnpg tfitkvelkc ppttekfqia csefldhyrd
      361 eeeevevevq q
//
LOCUS       XP_077505683             588 aa            linear   INV 25-NOV-2025
DEFINITION  CCR4-NOT transcription complex subunit 6-like twin isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077505683
VERSION     XP_077505683.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649557.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..588
                     /product="CCR4-NOT transcription complex subunit 6-like
                     twin isoform X1"
                     /calculated_mol_wt=67273
     Region          <74..>170
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          87..109
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          110..132
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          133..155
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          156..167
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          220..576
                     /region_name="Deadenylase_CCR4"
                     /note="C-terminal deadenylase domain of CCR4 and related
                     domains; cd09097"
                     /db_xref="CDD:197331"
     Site            order(224,269,391,394,396,402,450,452,454,518,523,
                     527..528,568)
                     /site_type="active"
                     /db_xref="CDD:197331"
     Site            order(224,269,391,394,396,402,450,452,454,518,523,527,568)
                     /site_type="other"
                     /note="poly(A) RNA binding site [nucleotide binding]"
                     /db_xref="CDD:197331"
     Site            order(224,269,391,450,452,528,567..568)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197331"
     Site            order(224,269,567)
                     /site_type="other"
                     /note="Mg binding site A [ion binding]"
                     /db_xref="CDD:197331"
     Site            order(391,452,568)
                     /site_type="other"
                     /note="putative phosphate binding site [ion binding]"
                     /db_xref="CDD:197331"
     Site            order(450,452,568)
                     /site_type="other"
                     /note="Mg binding site B [ion binding]"
                     /db_xref="CDD:197331"
     CDS             1..588
                     /gene="twin"
                     /coded_by="XM_077649557.1:110..1876"
                     /db_xref="GeneID:144115258"
ORIGIN      
        1 mvyyyypnnr ahtgrrrrgt sqqyslmsra alrererdrq eynparrtht imcpedyaag
       61 kkstwpelei tgiirnlspa lwslshlrcl ylndnslqri ppgigqlvcl thldlscnkl
      121 rslpaelgdl vtlrqlhlnh nhlrvlpyel grlfrlhtlg lkgnplspel lcmysepngt
      181 akllaylldn livgtthppq rpwvhvgnpt rsqpstvltv mcynvlcdky atrqvygycp
      241 twalsweyrr kgimdeirhy aadvislqev emeqfhlffl pelkkdgydg ifapksrakt
      301 maecdrkhvd gcaiffrlsk ftlikehlve fnqlamanad gsddmlnrvm tkdniglval
      361 lqfkegiven anpeykslvh qqppilvcta hihwdpeycd vkliqtmllm relrilaeea
      421 vqmyrsgslg gphrrlpida shiplllcgd mnslpesgvv eflkaghvss dhpdfkrlgy
      481 kdclrkmclq snslvgtmyt hpfkikeaye dgimpytnyt fdfkgvidyi fftrqhlgvl
      541 gvlgpldihw meenkvvgcp hphvpsdhlp lvaqlelllm snglllrr
//
LOCUS       XP_077505684             562 aa            linear   INV 25-NOV-2025
DEFINITION  CCR4-NOT transcription complex subunit 6-like twin isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077505684
VERSION     XP_077505684.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649558.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..562
                     /product="CCR4-NOT transcription complex subunit 6-like
                     twin isoform X2"
                     /calculated_mol_wt=64053
     Region          <48..>144
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          61..83
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          84..106
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          107..129
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          130..141
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          194..550
                     /region_name="Deadenylase_CCR4"
                     /note="C-terminal deadenylase domain of CCR4 and related
                     domains; cd09097"
                     /db_xref="CDD:197331"
     Site            order(198,243,365,368,370,376,424,426,428,492,497,
                     501..502,542)
                     /site_type="active"
                     /db_xref="CDD:197331"
     Site            order(198,243,365,368,370,376,424,426,428,492,497,501,542)
                     /site_type="other"
                     /note="poly(A) RNA binding site [nucleotide binding]"
                     /db_xref="CDD:197331"
     Site            order(198,243,365,424,426,502,541..542)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197331"
     Site            order(198,243,541)
                     /site_type="other"
                     /note="Mg binding site A [ion binding]"
                     /db_xref="CDD:197331"
     Site            order(365,426,542)
                     /site_type="other"
                     /note="putative phosphate binding site [ion binding]"
                     /db_xref="CDD:197331"
     Site            order(424,426,542)
                     /site_type="other"
                     /note="Mg binding site B [ion binding]"
                     /db_xref="CDD:197331"
     CDS             1..562
                     /gene="twin"
                     /coded_by="XM_077649558.1:361..2049"
                     /db_xref="GeneID:144115258"
ORIGIN      
        1 msraalrere rdrqeynpar rthtimcped yaagkkstwp eleitgiirn lspalwslsh
       61 lrclylndns lqrippgigq lvclthldls cnklrslpae lgdlvtlrql hlnhnhlrvl
      121 pyelgrlfrl htlglkgnpl spellcmyse pngtakllay lldnlivgtt hppqrpwvhv
      181 gnptrsqpst vltvmcynvl cdkyatrqvy gycptwalsw eyrrkgimde irhyaadvis
      241 lqevemeqfh lfflpelkkd gydgifapks raktmaecdr khvdgcaiff rlskftlike
      301 hlvefnqlam anadgsddml nrvmtkdnig lvallqfkeg ivenanpeyk slvhqqppil
      361 vctahihwdp eycdvkliqt mllmrelril aeeavqmyrs gslggphrrl pidashipll
      421 lcgdmnslpe sgvveflkag hvssdhpdfk rlgykdclrk mclqsnslvg tmythpfkik
      481 eayedgimpy tnytfdfkgv idyifftrqh lgvlgvlgpl dihwmeenkv vgcphphvps
      541 dhlplvaqle lllmsnglll rr
//
LOCUS       XP_077505685             562 aa            linear   INV 25-NOV-2025
DEFINITION  CCR4-NOT transcription complex subunit 6-like twin isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077505685
VERSION     XP_077505685.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649559.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..562
                     /product="CCR4-NOT transcription complex subunit 6-like
                     twin isoform X2"
                     /calculated_mol_wt=64053
     Region          <48..>144
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          61..83
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          84..106
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          107..129
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          130..141
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          194..550
                     /region_name="Deadenylase_CCR4"
                     /note="C-terminal deadenylase domain of CCR4 and related
                     domains; cd09097"
                     /db_xref="CDD:197331"
     Site            order(198,243,365,368,370,376,424,426,428,492,497,
                     501..502,542)
                     /site_type="active"
                     /db_xref="CDD:197331"
     Site            order(198,243,365,368,370,376,424,426,428,492,497,501,542)
                     /site_type="other"
                     /note="poly(A) RNA binding site [nucleotide binding]"
                     /db_xref="CDD:197331"
     Site            order(198,243,365,424,426,502,541..542)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197331"
     Site            order(198,243,541)
                     /site_type="other"
                     /note="Mg binding site A [ion binding]"
                     /db_xref="CDD:197331"
     Site            order(365,426,542)
                     /site_type="other"
                     /note="putative phosphate binding site [ion binding]"
                     /db_xref="CDD:197331"
     Site            order(424,426,542)
                     /site_type="other"
                     /note="Mg binding site B [ion binding]"
                     /db_xref="CDD:197331"
     CDS             1..562
                     /gene="twin"
                     /coded_by="XM_077649559.1:292..1980"
                     /db_xref="GeneID:144115258"
ORIGIN      
        1 msraalrere rdrqeynpar rthtimcped yaagkkstwp eleitgiirn lspalwslsh
       61 lrclylndns lqrippgigq lvclthldls cnklrslpae lgdlvtlrql hlnhnhlrvl
      121 pyelgrlfrl htlglkgnpl spellcmyse pngtakllay lldnlivgtt hppqrpwvhv
      181 gnptrsqpst vltvmcynvl cdkyatrqvy gycptwalsw eyrrkgimde irhyaadvis
      241 lqevemeqfh lfflpelkkd gydgifapks raktmaecdr khvdgcaiff rlskftlike
      301 hlvefnqlam anadgsddml nrvmtkdnig lvallqfkeg ivenanpeyk slvhqqppil
      361 vctahihwdp eycdvkliqt mllmrelril aeeavqmyrs gslggphrrl pidashipll
      421 lcgdmnslpe sgvveflkag hvssdhpdfk rlgykdclrk mclqsnslvg tmythpfkik
      481 eayedgimpy tnytfdfkgv idyifftrqh lgvlgvlgpl dihwmeenkv vgcphphvps
      541 dhlplvaqle lllmsnglll rr
//
LOCUS       XP_077505686             486 aa            linear   INV 25-NOV-2025
DEFINITION  CCR4-NOT transcription complex subunit 6-like twin isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077505686
VERSION     XP_077505686.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649560.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..486
                     /product="CCR4-NOT transcription complex subunit 6-like
                     twin isoform X3"
                     /calculated_mol_wt=54696
     Region          118..474
                     /region_name="Deadenylase_CCR4"
                     /note="C-terminal deadenylase domain of CCR4 and related
                     domains; cd09097"
                     /db_xref="CDD:197331"
     Site            order(122,167,289,292,294,300,348,350,352,416,421,
                     425..426,466)
                     /site_type="active"
                     /db_xref="CDD:197331"
     Site            order(122,167,289,292,294,300,348,350,352,416,421,425,466)
                     /site_type="other"
                     /note="poly(A) RNA binding site [nucleotide binding]"
                     /db_xref="CDD:197331"
     Site            order(122,167,289,348,350,426,465..466)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197331"
     Site            order(122,167,465)
                     /site_type="other"
                     /note="Mg binding site A [ion binding]"
                     /db_xref="CDD:197331"
     Site            order(289,350,466)
                     /site_type="other"
                     /note="putative phosphate binding site [ion binding]"
                     /db_xref="CDD:197331"
     Site            order(348,350,466)
                     /site_type="other"
                     /note="Mg binding site B [ion binding]"
                     /db_xref="CDD:197331"
     CDS             1..486
                     /gene="twin"
                     /coded_by="XM_077649560.1:16..1476"
                     /db_xref="GeneID:144115258"
ORIGIN      
        1 msrlchvaap lvcplerngs pvtlpplpta rrqprtppgr rpagwsrsag aalapflqra
       61 vangipaavh serccqhiad advklvvdkt fgtthppqrp wvhvgnptrs qpstvltvmc
      121 ynvlcdkyat rqvygycptw alsweyrrkg imdeirhyaa dvislqevem eqfhlfflpe
      181 lkkdgydgif apksraktma ecdrkhvdgc aiffrlskft likehlvefn qlamanadgs
      241 ddmlnrvmtk dniglvallq fkegivenan peykslvhqq ppilvctahi hwdpeycdvk
      301 liqtmllmre lrilaeeavq myrsgslggp hrrlpidash iplllcgdmn slpesgvvef
      361 lkaghvssdh pdfkrlgykd clrkmclqsn slvgtmythp fkikeayedg impytnytfd
      421 fkgvidyiff trqhlgvlgv lgpldihwme enkvvgcphp hvpsdhlplv aqlelllmsn
      481 glllrr
//
LOCUS       XP_077505687             581 aa            linear   INV 25-NOV-2025
DEFINITION  transmembrane 9 superfamily protein member 3 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505687
VERSION     XP_077505687.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649561.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..581
                     /product="transmembrane 9 superfamily protein member 3
                     isoform X1"
                     /calculated_mol_wt=66717
     Region          49..518
                     /region_name="EMP70"
                     /note="Endomembrane protein 70; pfam02990"
                     /db_xref="CDD:460774"
     CDS             1..581
                     /gene="TM9SF3"
                     /coded_by="XM_077649561.1:102..1847"
                     /db_xref="GeneID:144115259"
ORIGIN      
        1 mkrfgyafvl llicfrptes dehdhkyqdn eevvlwmntv gpyhnrqety tylslpfcvg
       61 pkasishyhe tlgeallgve lefsgldilf kapvaktayc eitlssaklq afiyavknhy
      121 wyqmyidglp iwgivgehde sdnsyylwth kkfeigyngn rivdvnltse vktklvlnqk
      181 ialtyeviwk pstvrfeerf dkyldpsffq hrihwfsifn sfmmviflvg lvsmilmrtl
      241 rkdyaryskd eemddmerdl gdeygwkqvh gdvfrpptht llfsalvgtg hqiavvvlcv
      301 ilfaimgely tergsllsta ifvyaatspi ngyfggslyg rmggkqwikq mlmsafllpa
      361 lvcgtafvin fiaiyyhasr aipfgtmvav tciclfiilp ltlvgtvlgr nlagqpnypc
      421 rinavprpip ekkwfmepsv ivvlggilpf gsifiemyfi ftsfwaykiy yvygfmllvf
      481 liltivticv tivctyflln aedyrwqwts flagastagy vylysfyyyf fktkmyglfq
      541 tvfyfgymal fsfalgvlcg tfgfigtsaf vrkiystvki d
//
LOCUS       XP_077505688             591 aa            linear   INV 25-NOV-2025
DEFINITION  transmembrane 9 superfamily protein member 3 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505688
VERSION     XP_077505688.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649562.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..591
                     /product="transmembrane 9 superfamily protein member 3
                     isoform X2"
                     /calculated_mol_wt=67871
     Region          49..528
                     /region_name="EMP70"
                     /note="Endomembrane protein 70; pfam02990"
                     /db_xref="CDD:460774"
     CDS             1..591
                     /gene="TM9SF3"
                     /coded_by="XM_077649562.1:84..1859"
                     /db_xref="GeneID:144115259"
ORIGIN      
        1 mkrfgyafvl llicfrptes dehdhkyqdn eevvlwmntv gpyhnrqety tylslpfcvg
       61 pkasishyhe tlgeallgve lefsgldilf kapvaktayc eitlssaklq afiyavknhy
      121 wyqmyidglp iwgivgehde sdnsyylwth kkfeigyngn rivdvnltse vktklvlnqk
      181 ialtyeviwk pstvrfeerf dkyldpsffq hrihwfsifn sfmmviflvg lvsmilmrtl
      241 rkdyaryskd eemddmvgne wqivqverdl gdeygwkqvh gdvfrpptht llfsalvgtg
      301 hqiavvvlcv ilfaimgely tergsllsta ifvyaatspi ngyfggslyg rmggkqwikq
      361 mlmsafllpa lvcgtafvin fiaiyyhasr aipfgtmvav tciclfiilp ltlvgtvlgr
      421 nlagqpnypc rinavprpip ekkwfmepsv ivvlggilpf gsifiemyfi ftsfwaykiy
      481 yvygfmllvf liltivticv tivctyflln aedyrwqwts flagastagy vylysfyyyf
      541 fktkmyglfq tvfyfgymal fsfalgvlcg tfgfigtsaf vrkiystvki d
//
LOCUS       XP_077505689            1931 aa            linear   INV 25-NOV-2025
DEFINITION  sec7 domain-containing protein garz isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505689
VERSION     XP_077505689.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649563.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1931
                     /product="sec7 domain-containing protein garz isoform X1"
                     /calculated_mol_wt=211266
     Region          415..571
                     /region_name="Sec7_N"
                     /note="Guanine nucleotide exchange factor in Golgi
                     transport N-terminal; pfam12783"
                     /db_xref="CDD:463703"
     Region          <557..>1055
                     /region_name="PLN03076"
                     /note="ARF guanine nucleotide exchange factor (ARF-GEF);
                     Provisional"
                     /db_xref="CDD:215560"
     Region          718..903
                     /region_name="Sec7"
                     /note="Sec7 domain; pfam01369"
                     /db_xref="CDD:460178"
     Region          <1592..>1669
                     /region_name="PLN03076"
                     /note="ARF guanine nucleotide exchange factor (ARF-GEF);
                     Provisional"
                     /db_xref="CDD:215560"
     CDS             1..1931
                     /gene="garz"
                     /coded_by="XM_077649563.1:168..5963"
                     /db_xref="GeneID:144115260"
ORIGIN      
        1 myvpenhiyi iagevsvita alrrsssgqs nqaeehealt rsfsnlkdal grstdisdie
       61 psvyfgpfle airsddtsgi vtgqalasvn kflsyglldh rlessasaae siadavthar
      121 fmgidpasde vvlmkillvl rslflshvga llsnesvcem mqsciricfe trlsellrks
      181 aeqalmdmvq llfsrlpqfs evakggcskk lkmrasgmdn arnarkrrsp vpkhrkipre
      241 hdsqaqqqth avsqspanva sqpqtpttde qppvgetvgs gvskvplpgp gstsesefpp
      301 asdsslepgp ltgvihlsds vpsivdldts qadslscaqe gldsvlsmsq dltgseynlv
      361 aqepsslets iekestgtvv tmggtgdndy vnphgvrfta lhggregagp vvpyglpcvw
      421 ellrflasll yvpeavghnq qqqqslemmi hsglnllava lesgadhiss fpsllslvkd
      481 dvcrnllmll sssrlsifvs slrvsfllfe alrthlkfql emyltklmel ivsespnvtr
      541 dqkelsveav lqfwripgli telylnydcd lfcsnlfedl tkvlsknafp vtglqpihll
      601 sldallavid sietrchfrm lsetqaargg smsgslslld gdgegyhgep aavveqchva
      661 pfgyqlgqql vhcpdgalqs ptsgtkphfr ssvfmrpnrk svsenipshe elmaikhkkk
      721 llasgtehfn srpskgiefl qehgllsdpl dpaevalflr dnsqldkkki geyianrknl
      781 kvldafvksf nfantridea lrmyletfrl pgeaplisll mehfaehwhk smnepfvnsd
      841 aaftlayavi mlnmdqhnhn vkkqnipmtv edfkknlkdv nggkdfdkdm leeiynaikn
      901 eeivmpaeqt glvrenylwk vllrrgaska grfvhapngl ldhdlftlvw gptvaalasv
      961 ldrapseslv lqralggyrk camvaahyam sdvfdnlvis lckftslsts espetvplal
     1021 gssqkaqlva kmvfglaqrh ghilrdgwkn lvdcllqlyk akllpralvt aedfvdpsge
     1081 vslvraeegp stgpgqqqfn ifnsfymylt dssqrgpnpe dlrareaalt cvancqpell
     1141 vseskflred alqelvkali ytchgpeshs smsggydehs tvfllellik vvlqnkdrvg
     1201 piwtavrdhl ytlvmgasas dyrflldrav vgilrlairl irreemtsqv lgslqlllll
     1261 rpatlqqvcg qvacalqell rtsatcvqap sdwatlfvlm acagaglkpq aahalhrppa
     1321 fhrsmtsvad stttsvgaqs dselsravhg rqegsidrgy tsdselyqhq sangdaarvp
     1381 sscqddcpkg amdssaggwi lvsgggaeee rshsaapvnq fsislacdll phdpfalakc
     1441 ceclaflvrd lahitpsnlh gciqcirvfv easlhggnnw akrnakpret krmtkgqarr
     1501 reealhrrnq qhslgyeadd edrlegapse yqhvslqlld lmhtlhtraa silqpndlvp
     1561 elvlsstglp vsgdmsrlar gsqqptlwnl cwcpllqgia rlccdtrrni rtsaltylqr
     1621 allvhdlqal saaeweacfn kvlfpllskl menvspndpv gmeetrmrga tllckvflqh
     1681 lnpllslptf talwltildf mdkymhaeds dllseaipes lknmllvmdt agvfqaagee
     1741 lensssgytq lwtvtwdrid sflprlkdev fkppprpptp palpstipce eaapesgcss
     1801 dqavrtaqee dsyelvsvmv edpsfmerrp ssasvgsecs sitlgasqtg lagtmafsei
     1861 pagpvaapvd pvspphsvil hppslspppv lptavpptat pvplvidpiv fceqhhsvfa
     1921 psqnpvksad r
//
LOCUS       XP_077505690            1930 aa            linear   INV 25-NOV-2025
DEFINITION  sec7 domain-containing protein garz isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505690
VERSION     XP_077505690.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649564.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1930
                     /product="sec7 domain-containing protein garz isoform X2"
                     /calculated_mol_wt=211179
     Region          415..571
                     /region_name="Sec7_N"
                     /note="Guanine nucleotide exchange factor in Golgi
                     transport N-terminal; pfam12783"
                     /db_xref="CDD:463703"
     Region          <557..>1055
                     /region_name="PLN03076"
                     /note="ARF guanine nucleotide exchange factor (ARF-GEF);
                     Provisional"
                     /db_xref="CDD:215560"
     Region          718..903
                     /region_name="Sec7"
                     /note="Sec7 domain; pfam01369"
                     /db_xref="CDD:460178"
     Region          <1591..>1668
                     /region_name="PLN03076"
                     /note="ARF guanine nucleotide exchange factor (ARF-GEF);
                     Provisional"
                     /db_xref="CDD:215560"
     CDS             1..1930
                     /gene="garz"
                     /coded_by="XM_077649564.1:168..5960"
                     /db_xref="GeneID:144115260"
ORIGIN      
        1 myvpenhiyi iagevsvita alrrsssgqs nqaeehealt rsfsnlkdal grstdisdie
       61 psvyfgpfle airsddtsgi vtgqalasvn kflsyglldh rlessasaae siadavthar
      121 fmgidpasde vvlmkillvl rslflshvga llsnesvcem mqsciricfe trlsellrks
      181 aeqalmdmvq llfsrlpqfs evakggcskk lkmrasgmdn arnarkrrsp vpkhrkipre
      241 hdsqaqqqth avsqspanva sqpqtpttde qppvgetvgs gvskvplpgp gstsesefpp
      301 asdsslepgp ltgvihlsds vpsivdldts qadslscaqe gldsvlsmsq dltgseynlv
      361 aqepsslets iekestgtvv tmggtgdndy vnphgvrfta lhggregagp vvpyglpcvw
      421 ellrflasll yvpeavghnq qqqqslemmi hsglnllava lesgadhiss fpsllslvkd
      481 dvcrnllmll sssrlsifvs slrvsfllfe alrthlkfql emyltklmel ivsespnvtr
      541 dqkelsveav lqfwripgli telylnydcd lfcsnlfedl tkvlsknafp vtglqpihll
      601 sldallavid sietrchfrm lsetqaargg smsgslslld gdgegyhgep aavveqchva
      661 pfgyqlgqql vhcpdgalqs ptsgtkphfr ssvfmrpnrk svsenipshe elmaikhkkk
      721 llasgtehfn srpskgiefl qehgllsdpl dpaevalflr dnsqldkkki geyianrknl
      781 kvldafvksf nfantridea lrmyletfrl pgeaplisll mehfaehwhk smnepfvnsd
      841 aaftlayavi mlnmdqhnhn vkkqnipmtv edfkknlkdv nggkdfdkdm leeiynaikn
      901 eeivmpaeqt glvrenylwk vllrrgaska grfvhapngl ldhdlftlvw gptvaalasv
      961 ldrapseslv lqralggyrk camvaahyam sdvfdnlvis lckftslsts espetvplal
     1021 gssqkaqlva kmvfglaqrh ghilrdgwkn lvdcllqlyk akllpralvt aedfvdpsge
     1081 vslvraeegp stgpgqqqfn ifnsfymylt dsqrgpnped lrareaaltc vancqpellv
     1141 seskflreda lqelvkaliy tchgpeshss msggydehst vfllellikv vlqnkdrvgp
     1201 iwtavrdhly tlvmgasasd yrflldravv gilrlairli rreemtsqvl gslqlllllr
     1261 patlqqvcgq vacalqellr tsatcvqaps dwatlfvlma cagaglkpqa ahalhrppaf
     1321 hrsmtsvads tttsvgaqsd selsravhgr qegsidrgyt sdselyqhqs angdaarvps
     1381 scqddcpkga mdssaggwil vsgggaeeer shsaapvnqf sislacdllp hdpfalakcc
     1441 eclaflvrdl ahitpsnlhg ciqcirvfve aslhggnnwa krnakpretk rmtkgqarrr
     1501 eealhrrnqq hslgyeadde drlegapsey qhvslqlldl mhtlhtraas ilqpndlvpe
     1561 lvlsstglpv sgdmsrlarg sqqptlwnlc wcpllqgiar lccdtrrnir tsaltylqra
     1621 llvhdlqals aaeweacfnk vlfpllsklm envspndpvg meetrmrgat llckvflqhl
     1681 npllslptft alwltildfm dkymhaedsd llseaipesl knmllvmdta gvfqaageel
     1741 ensssgytql wtvtwdrids flprlkdevf kppprpptpp alpstipcee aapesgcssd
     1801 qavrtaqeed syelvsvmve dpsfmerrps sasvgsecss itlgasqtgl agtmafseip
     1861 agpvaapvdp vspphsvilh ppslspppvl ptavpptatp vplvidpivf ceqhhsvfap
     1921 sqnpvksadr
//
LOCUS       XP_077505691             657 aa            linear   INV 25-NOV-2025
DEFINITION  histidine ammonia-lyase-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505691
VERSION     XP_077505691.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649565.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..657
                     /product="histidine ammonia-lyase-like isoform X1"
                     /calculated_mol_wt=72257
     Region          2..86
                     /region_name="Par3_HAL_N_term"
                     /note="N-terminal of Par3 and HAL proteins; pfam12053"
                     /db_xref="CDD:463446"
     Region          111..616
                     /region_name="HutH"
                     /note="Histidine ammonia-lyase [Amino acid transport and
                     metabolism]; COG2986"
                     /db_xref="CDD:442225"
     CDS             1..657
                     /gene="LOC144115262"
                     /coded_by="XM_077649565.1:206..2179"
                     /db_xref="GeneID:144115262"
ORIGIN      
        1 mkvsvrvrge wlavpcrdgr qtvrwlgeea lrrymklkps syldgrtekv yqirktrgga
       61 iidgddvirn vlddneflsv vletdrpspv tgpaeityvp eqvpgkfkap tqmvtidgnn
      121 ltpgvllelg rgkykiqlsg dsiervkmar emletilken kvaygintgf gkfarvvipd
      181 dnlkelqenl vrshaagvgn plspektrml lalrinvlsk gysgvslecl qqlveafnas
      241 clswvpeqgt vgasgdlapl ahlalglige gkmwspetgw gdaqyvlesh klkpiqlkpk
      301 eglcllngtq litsigaeav qraiilsqqa dvvaaltlev lkgttrafes diqkirphwg
      361 qglvakrlra llhsdtfpse iaeshrfchr vqdaytlrcc pqvhgivhdt idfvrgiitt
      421 emnsatdnpl vfcergeiis ggnfhgeypa kaldylaigv helgsmserr terlinpays
      481 elpaflvadg glnsgfmiah ctaaalvsen kalchpasvd svptsagted hvsmggfaar
      541 kalqvvqnve riiaiellca cqaieflrpl kttapleavy klvrmfvkpw dkdrvmapdi
      601 daatdllkqs rlwdivkphm dhyftvqeee trmpspttse isvassgkkr kvdkhdf
//
LOCUS       XP_077505692             660 aa            linear   INV 25-NOV-2025
DEFINITION  histidine ammonia-lyase-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505692
VERSION     XP_077505692.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649566.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..660
                     /product="histidine ammonia-lyase-like isoform X2"
                     /calculated_mol_wt=72544
     Region          2..86
                     /region_name="Par3_HAL_N_term"
                     /note="N-terminal of Par3 and HAL proteins; pfam12053"
                     /db_xref="CDD:463446"
     Region          114..619
                     /region_name="HutH"
                     /note="Histidine ammonia-lyase [Amino acid transport and
                     metabolism]; COG2986"
                     /db_xref="CDD:442225"
     CDS             1..660
                     /gene="LOC144115262"
                     /coded_by="XM_077649566.1:231..2213"
                     /db_xref="GeneID:144115262"
ORIGIN      
        1 mkvsvrvrge wlavpcrdgr qtvrwlgeea lrrymklkps syldgrtekv yqirktrgga
       61 iidgddvirn vlddneflsv vletdrpspv tgpaeityvp eqvyplqiaa nlgaemvtid
      121 gnnltpgvll elgrgkykiq lsgdsiervk maremletil kenkvaygin tgfgkfarvv
      181 ipddnlkelq enlvrshaag vgnplspekt rmllalrinv lskgysgvsl eclqqlveaf
      241 nasclswvpe qgtvgasgdl aplahlalgl igegkmwspe tgwgdaqyvl eshklkpiql
      301 kpkeglclln gtqlitsiga eavqraiils qqadvvaalt levlkgttra fesdiqkirp
      361 hwgqglvakr lrallhsdtf pseiaeshrf chrvqdaytl rccpqvhgiv hdtidfvrgi
      421 ittemnsatd nplvfcerge iisggnfhge ypakaldyla igvhelgsms errterlinp
      481 ayselpaflv adgglnsgfm iahctaaalv senkalchpa svdsvptsag tedhvsmggf
      541 aarkalqvvq nveriiaiel lcacqaiefl rplkttaple avyklvrmfv kpwdkdrvma
      601 pdidaatdll kqsrlwdivk phmdhyftvq eeetrmpspt tseisvassg kkrkvdkhdf
//
LOCUS       XP_077505693             483 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115263 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505693
VERSION     XP_077505693.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649567.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..483
                     /product="uncharacterized protein LOC144115263 isoform X1"
                     /calculated_mol_wt=53192
     Region          <250..>338
                     /region_name="PRK00409"
                     /note="recombination and DNA strand exchange inhibitor
                     protein; Reviewed"
                     /db_xref="CDD:234750"
     CDS             1..483
                     /gene="LOC144115263"
                     /coded_by="XM_077649567.1:157..1608"
                     /db_xref="GeneID:144115263"
ORIGIN      
        1 mdtgkliahp svrmrgseht fepiecsnek ctaqpvphlh cpfctknsvf kdprivkahy
       61 rvkhvdkgly faglkmlrcs atcrisgsik gekkfrgphw hcykckngfc rrdeamkhfe
      121 thfkmpqttf qinivqdvne gmglqsrlds savehsechm ssasdistdm dsntavavtv
      181 dmteessaen paspstsetp ttvmiiqeed trslssdpgc mlktreedla ekniflqkll
      241 tefkqqmdnq karhthsena mqleilslrr qlekknaele qlkkqqagll sgktpqlnss
      301 lqkllkgmqn qhaellmqqi aqarneafqs alnelqngtt ilttvensst tvlqqennpl
      361 vamvlnttgt lgipvslptn nnpsvvcspv knllqklgat gvpllisgqq tfvqpqpgrs
      421 vqqavgsqnl vltladtsdv sqdlvssaqh gntvvactdl qyqlpavtsd skddqenvee
      481 cts
//
LOCUS       XP_077505694             438 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115263 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505694
VERSION     XP_077505694.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649568.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..438
                     /product="uncharacterized protein LOC144115263 isoform X2"
                     /calculated_mol_wt=47785
     Region          <226..326
                     /region_name="HlpA"
                     /note="Periplasmic chaperone for outer membrane proteins,
                     Skp family [Cell wall/membrane/envelope biogenesis,
                     Posttranslational modification, protein turnover,
                     chaperones]; COG2825"
                     /db_xref="CDD:442073"
     CDS             1..438
                     /gene="LOC144115263"
                     /coded_by="XM_077649568.1:158..1474"
                     /db_xref="GeneID:144115263"
ORIGIN      
        1 mdtgkliahp svrmrgseht fepiecsnek ctaqpvphlh cpfctknsvf kdprivkahy
       61 rvkhvdkgly faglkmlrcs atcrisgsik gekkfrgphw hcykckngfc rrdeamkhfe
      121 thfkmpqttf qinivqdvne gmglqsrlds savehsechm ssasdistdm dsntavavtv
      181 dmteessaen paspstsetp ttvmiiqeed trslssdpgc mlktrelekk naeleqlkkq
      241 qagllsgktp qlnsslqkll kgmqnqhael lmqqiaqarn eafqsalnel qngttilttv
      301 enssttvlqq ennplvamvl nttgtlgipv slptnnnpsv vcspvknllq klgatgvpll
      361 isgqqtfvqp qpgrsvqqav gsqnlvltla dtsdvsqdlv ssaqhgntvv actdlqyqlp
      421 avtsdskddq enveects
//
LOCUS       XP_077505695             356 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115263 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505695
VERSION     XP_077505695.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649569.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..356
                     /product="uncharacterized protein LOC144115263 isoform X3"
                     /calculated_mol_wt=38396
     Region          <123..>211
                     /region_name="PRK00409"
                     /note="recombination and DNA strand exchange inhibitor
                     protein; Reviewed"
                     /db_xref="CDD:234750"
     CDS             1..356
                     /gene="LOC144115263"
                     /coded_by="XM_077649569.1:343..1413"
                     /db_xref="GeneID:144115263"
ORIGIN      
        1 mstkdstsld vnegmglqsr ldssavehse chmssasdis tdmdsntava vtvdmteess
       61 aenpaspsts etpttvmiiq eedtrslssd pgcmlktree dlaekniflq klltefkqqm
      121 dnqkarhths enamqleils lrrqlekkna eleqlkkqqa gllsgktpql nsslqkllkg
      181 mqnqhaellm qqiaqarnea fqsalnelqn gttilttven ssttvlqqen nplvamvlnt
      241 tgtlgipvsl ptnnnpsvvc spvknllqkl gatgvpllis gqqtfvqpqp grsvqqavgs
      301 qnlvltladt sdvsqdlvss aqhgntvvac tdlqyqlpav tsdskddqen veects
//
LOCUS       XP_077505696             105 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115265 [Amblyomma americanum].
ACCESSION   XP_077505696
VERSION     XP_077505696.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649570.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..105
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..105
                     /product="uncharacterized protein LOC144115265"
                     /calculated_mol_wt=11609
     CDS             1..105
                     /gene="LOC144115265"
                     /coded_by="XM_077649570.1:147..464"
                     /db_xref="GeneID:144115265"
ORIGIN      
        1 mptlyylaaa svfslrradr adqqcqsvsa ssmvsagflt pnliqtsqlp ilcckrtqlr
       61 gvrrqtgmvc lqhskrilmt altnqvwtpq qllplnhhhl gsslf
//
LOCUS       XP_077505697             941 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115266 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505697
VERSION     XP_077505697.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649571.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..941
                     /product="uncharacterized protein LOC144115266 isoform X1"
                     /calculated_mol_wt=100787
     CDS             1..941
                     /gene="LOC144115266"
                     /coded_by="XM_077649571.1:36..2861"
                     /db_xref="GeneID:144115266"
ORIGIN      
        1 mcitvmrwpf pgsllqamvv lilaqaahah csvlpptlrl lplhishpgn iqgavdgpfs
       61 llmciammrw pfpgsllqam vvlilaqaah ahcsvlpptl rllplhishp gniqgavdgp
      121 fsllmcitmm rwpfpgsllq amvvlilaqa ahahcsvlpp tlrllplhis hpgniqgavd
      181 gpfsllmcia mmrwpfpgsl lqamvvlila qaahahcsvl pptlrllplh ishpgniqga
      241 vdgpfsllmc lammrwpfpg sllqamvvli laqaahahcs vlpptlrllp lhishpgniq
      301 gavdgpfsll mcitmmrwpf pgsllqamvv lilaqaahah csvlpptlrl lplhishpgn
      361 ipgavdgpfs llmcitvmrw pfpgsllqam vvlilaqaah arcsvlpptl rllplhishp
      421 gniqgavdgp fsllmcitmm rwpfpgsllq amvvlilaqa aharcsvlpp tlrllplhis
      481 hpgnipgavd gpfsvlmcit mmrwpfpgsl lqamvvlila qaaharcsvl pptlrllplh
      541 ishpgniqga vdgpfsllmc itmmrwpfpg sllqamvvli laqaaharcs vlpptlrllp
      601 lhishpgnip gavdgpfsvl mcitmmrwpf pgsllqamvv lilaqaahar csvlpptlrl
      661 lplhishpgn ipgavdgpfs llmcitvmrw pfpgsllqam vvlilaqaah arcsvlpptl
      721 rllplhishp gnipgavdgp fsllmcitvm rwpfpgsllq amvvlilaqa aharcsvlpp
      781 llhllplhis hpgnipgavd gpfsllmcit vmrwpfpgsl lqamvvhtla qaaharcsvl
      841 pptlrllplh ishpgnipga vdgpfsllmc itvmrwpfpg sllqamvvli laqaaharcs
      901 vlpptlrllp lhisqpvffg alhcgvpdsl shlgtlhphk t
//
LOCUS       XP_077505698             805 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115266 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505698
VERSION     XP_077505698.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649572.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..805
                     /product="uncharacterized protein LOC144115266 isoform X2"
                     /calculated_mol_wt=86379
     CDS             1..805
                     /gene="LOC144115266"
                     /coded_by="XM_077649572.1:1495..3912"
                     /db_xref="GeneID:144115266"
ORIGIN      
        1 mqqqccvqvs aymwqalhga nwslpqrpaa gtqqqqrniq gavdgpfsll mciammrwpf
       61 pgsllqamvv lilaqaahah csvlpptlrl lplhishpgn iqgavdgpfs llmclammrw
      121 pfpgsllqam vvlilaqaah ahcsvlpptl rllplhishp gniqgavdgp fsllmcitmm
      181 rwpfpgsllq amvvlilaqa ahahcsvlpp tlrllplhis hpgnipgavd gpfsllmcit
      241 vmrwpfpgsl lqamvvlila qaaharcsvl pptlrllplh ishpgniqga vdgpfsllmc
      301 itmmrwpfpg sllqamvvli laqaaharcs vlpptlrllp lhishpgnip gavdgpfsvl
      361 mcitmmrwpf pgsllqamvv lilaqaahar csvlpptlrl lplhishpgn iqgavdgpfs
      421 llmcitmmrw pfpgsllqam vvlilaqaah arcsvlpptl rllplhishp gnipgavdgp
      481 fsvlmcitmm rwpfpgsllq amvvlilaqa aharcsvlpp tlrllplhis hpgnipgavd
      541 gpfsllmcit vmrwpfpgsl lqamvvlila qaaharcsvl pptlrllplh ishpgnipga
      601 vdgpfsllmc itvmrwpfpg sllqamvvli laqaaharcs vlppllhllp lhishpgnip
      661 gavdgpfsll mcitvmrwpf pgsllqamvv htlaqaahar csvlpptlrl lplhishpgn
      721 ipgavdgpfs llmcitvmrw pfpgsllqam vvlilaqaah arcsvlpptl rllplhisqp
      781 vffgalhcgv pdslshlgtl hphkt
//
LOCUS       XP_077505699             755 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115266 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505699
VERSION     XP_077505699.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649573.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..755
                     /product="uncharacterized protein LOC144115266 isoform X3"
                     /calculated_mol_wt=80854
     CDS             1..755
                     /gene="LOC144115266"
                     /coded_by="XM_077649573.1:303..2570"
                     /db_xref="GeneID:144115266"
ORIGIN      
        1 mciammrwpf pgsllqamvv lilaqaahah csvlpptlrl lplhishpgn iqgavdgpfs
       61 llmclammrw pfpgsllqam vvlilaqaah ahcsvlpptl rllplhishp gniqgavdgp
      121 fsllmcitmm rwpfpgsllq amvvlilaqa ahahcsvlpp tlrllplhis hpgnipgavd
      181 gpfsllmcit vmrwpfpgsl lqamvvlila qaaharcsvl pptlrllplh ishpgniqga
      241 vdgpfsllmc itmmrwpfpg sllqamvvli laqaaharcs vlpptlrllp lhishpgnip
      301 gavdgpfsvl mcitmmrwpf pgsllqamvv lilaqaahar csvlpptlrl lplhishpgn
      361 iqgavdgpfs llmcitmmrw pfpgsllqam vvlilaqaah arcsvlpptl rllplhishp
      421 gnipgavdgp fsvlmcitmm rwpfpgsllq amvvlilaqa aharcsvlpp tlrllplhis
      481 hpgnipgavd gpfsllmcit vmrwpfpgsl lqamvvlila qaaharcsvl pptlrllplh
      541 ishpgnipga vdgpfsllmc itvmrwpfpg sllqamvvli laqaaharcs vlppllhllp
      601 lhishpgnip gavdgpfsll mcitvmrwpf pgsllqamvv htlaqaahar csvlpptlrl
      661 lplhishpgn ipgavdgpfs llmcitvmrw pfpgsllqam vvlilaqaah arcsvlpptl
      721 rllplhisqp vffgalhcgv pdslshlgtl hphkt
//
LOCUS       XP_077505700             755 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115266 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505700
VERSION     XP_077505700.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649574.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..755
                     /product="uncharacterized protein LOC144115266 isoform X3"
                     /calculated_mol_wt=80854
     CDS             1..755
                     /gene="LOC144115266"
                     /coded_by="XM_077649574.1:140..2407"
                     /db_xref="GeneID:144115266"
ORIGIN      
        1 mciammrwpf pgsllqamvv lilaqaahah csvlpptlrl lplhishpgn iqgavdgpfs
       61 llmclammrw pfpgsllqam vvlilaqaah ahcsvlpptl rllplhishp gniqgavdgp
      121 fsllmcitmm rwpfpgsllq amvvlilaqa ahahcsvlpp tlrllplhis hpgnipgavd
      181 gpfsllmcit vmrwpfpgsl lqamvvlila qaaharcsvl pptlrllplh ishpgniqga
      241 vdgpfsllmc itmmrwpfpg sllqamvvli laqaaharcs vlpptlrllp lhishpgnip
      301 gavdgpfsvl mcitmmrwpf pgsllqamvv lilaqaahar csvlpptlrl lplhishpgn
      361 iqgavdgpfs llmcitmmrw pfpgsllqam vvlilaqaah arcsvlpptl rllplhishp
      421 gnipgavdgp fsvlmcitmm rwpfpgsllq amvvlilaqa aharcsvlpp tlrllplhis
      481 hpgnipgavd gpfsllmcit vmrwpfpgsl lqamvvlila qaaharcsvl pptlrllplh
      541 ishpgnipga vdgpfsllmc itvmrwpfpg sllqamvvli laqaaharcs vlppllhllp
      601 lhishpgnip gavdgpfsll mcitvmrwpf pgsllqamvv htlaqaahar csvlpptlrl
      661 lplhishpgn ipgavdgpfs llmcitvmrw pfpgsllqam vvlilaqaah arcsvlpptl
      721 rllplhisqp vffgalhcgv pdslshlgtl hphkt
//
LOCUS       XP_077505701             468 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115268 [Amblyomma americanum].
ACCESSION   XP_077505701
VERSION     XP_077505701.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649575.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..468
                     /product="uncharacterized protein LOC144115268"
                     /calculated_mol_wt=49933
     Region          <95..>250
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     CDS             1..468
                     /gene="LOC144115268"
                     /coded_by="XM_077649575.1:1582..2988"
                     /db_xref="GeneID:144115268"
ORIGIN      
        1 msrglatsra npplwisaik hediiiiiss qllelsvler hetsngrhlf frtvpscgta
       61 klgastpfla tspmlssfrv vlyissrcrt tlakeltaav raraagtsqp vprfhlgshr
      121 mpsspsassp rgqgaqvpsq qpvprghlgs hrtpsspsas lprrhgtqvp sqqpvprghl
      181 gshrtsssps aslprghgaq vpsqqpvprg hlgslrtpss psasaprvha aqvpsqqpvp
      241 rghlgshrts trqalrrlga teqkyhrsnl craaisaasg rrarqalrcl gatehkyhrs
      301 nlcraaisva tgrqarqalr clgatehkyh rsnlcraais aatgrrarqa lrclgatehk
      361 yhrsnlcraa isaasgrqar qalrrlgatl hkyhlsnlcr aaisaatgrr lakrfgasgp
      421 rskstiaatc aarpsrqppd aelakrfvas gprststiav tcaarpsr
//
LOCUS       XP_077505702             194 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115269 [Amblyomma americanum].
ACCESSION   XP_077505702
VERSION     XP_077505702.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649576.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..194
                     /product="uncharacterized protein LOC144115269"
                     /calculated_mol_wt=20915
     Region          <52..150
                     /region_name="NADB_Rossmann"
                     /note="Rossmann-fold NAD(P)(+)-binding proteins; cl21454"
                     /db_xref="CDD:473865"
     CDS             1..194
                     /gene="LOC144115269"
                     /coded_by="XM_077649576.1:2182..2766"
                     /db_xref="GeneID:144115269"
ORIGIN      
        1 mwhrevrcvh tiprdqsdae llqsgsvhql amqndlgqga drccksescr niatcaalps
       61 gqppdaelvk rfvasgprst stiaatcaar psrqppdael akrfvasgpr ststiaatca
      121 arpsrqppda elakrfvasg prststisgt caarrssrsp frrlylkfmg vlrefvgscy
      181 avergddsfd fvfy
//
LOCUS       XP_077505703            1556 aa            linear   INV 25-NOV-2025
DEFINITION  nuclear pore complex protein Nup133-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505703
VERSION     XP_077505703.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649577.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1556
                     /product="nuclear pore complex protein Nup133-like isoform
                     X1"
                     /calculated_mol_wt=174504
     Region          95..453
                     /region_name="Nucleoporin_N"
                     /note="Nup133 N terminal like; pfam08801"
                     /db_xref="CDD:400932"
     Region          520..878
                     /region_name="Nucleoporin_N"
                     /note="Nup133 N terminal like; pfam08801"
                     /db_xref="CDD:400932"
     Region          1047..1487
                     /region_name="Nucleoporin_C"
                     /note="Non-repetitive/WGA-negative nucleoporin C-terminal;
                     pfam03177"
                     /db_xref="CDD:427181"
     CDS             1..1556
                     /gene="LOC144115270"
                     /coded_by="XM_077649577.1:85..4755"
                     /db_xref="GeneID:144115270"
ORIGIN      
        1 mftprragsa rgtfltesds lprsgrrgml psygrspymh rsqlhesplh qsqvveetan
       61 hvveifgtel pvlvsealie aepgtdittr isscgwawlv cgrklcvwqy kkglhnrncr
      121 vlmlppsdla hradlvcvll rgelapaalv vapagilryw pniahedcsi daladlhgee
      181 ccsltntepl gcivatttss lmlvtpstie gqttllikpl ktpqglltgi gqqvssfifg
      241 tmpaqleary lakvlveptm eeggkyfyvl lrnslqkwfl kdkdpeklcg ecdldrcmke
      301 qfhetvwngs ntlpmhlkaw cidmqlpsng vailmaalnp hvsqrlyyai gtvdirslgs
      361 padrpasftd ftvlrfqeqy tekdeealls ykfvlpsamc pssfvysgtk vycvmsgsdv
      421 gdldviefkk dkllgagcye etplffsrvn givcirsvha tardarsqlh esplhqsqvl
      481 eetanhvvei fgtelpvlvs ealieaepgt dittrisscg wawlvcgrkl cvwqykkglh
      541 nrnchvlmlp psdlahradl vcvllrgqla paalvvapag ilrywpniah edcsidalad
      601 lhgeeccslt nteplgciva tttsslmlvt pstiegqttl likplktpqg lltgigqqvs
      661 sfifgtmpaq learylakvl veptmeeggk yfyvllrnsl qkwflkdkdp eklcgecdld
      721 rcmkeqfhet vwngsntlpm hlkawcidmq lpsngvailm aalnphvsqr lyyaigtvdv
      781 rslgspadcp asftdftvlr fqeqytekde eallsykfvl psamcpssfv ysgtkvycvm
      841 sgsdvgdldv iefkkdkllg agcyeetplf fsrvngivci rsvhatarda slamelmpec
      901 pysladdetm egisvdernl hklrsafalf cqnellksqe ivnnifprpv elfrdldsal
      961 dtcvsqlgyw lmdgvpvmdp rwtqlqfpgg svtvssasll ihrqledklq ahqlflnflk
     1021 avhlwprlya vtvrgspmat nlllqeqaek lvaamelhsl qaqfntiida girrviqecs
     1081 astpggltaa dyfyrkvsel dkifpalvee eedqlkmgls prdefalitn vntifvwvlr
     1141 dvclfrdreq ltyecarets lefrpwttil rdiickqhtl attnaiavae natsfgqvfl
     1201 qltdladlil nghkvqldsl thnqaayeal klqyqqdrrh fiaplikatq yeraatlaek
     1261 hcdfeslvei celtrnkerl esymvlfkdq gfpefvfqwq ldsgrrgell dqpasqhgnl
     1321 erfleghdsl swlhdihlgk mgkaattlhr lgqqeeqnml rkkyllshsk laalcsddpp
     1381 ethadlikni nreealityq eslpfavkea ycleprgmkv lgpeeliemy istanlnane
     1441 ydftkaleil dfiedptraq elrmriwcaa ilrnkwenld tdnvegimkd rlffriadlg
     1501 vtqgrnlaqy lpplegllem pdlqdlrsnh hflfilgagy ehlqrmaaik tslhqa
//
LOCUS       XP_077505704            1434 aa            linear   INV 25-NOV-2025
DEFINITION  nuclear pore complex protein Nup133-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505704
VERSION     XP_077505704.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649578.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1434
                     /product="nuclear pore complex protein Nup133-like isoform
                     X2"
                     /calculated_mol_wt=160784
     Region          <54..331
                     /region_name="Nucleoporin_N"
                     /note="Nup133 N terminal like; pfam08801"
                     /db_xref="CDD:400932"
     Region          398..756
                     /region_name="Nucleoporin_N"
                     /note="Nup133 N terminal like; pfam08801"
                     /db_xref="CDD:400932"
     Region          925..1365
                     /region_name="Nucleoporin_C"
                     /note="Non-repetitive/WGA-negative nucleoporin C-terminal;
                     pfam03177"
                     /db_xref="CDD:427181"
     CDS             1..1434
                     /gene="LOC144115270"
                     /coded_by="XM_077649578.1:1142..5446"
                     /db_xref="GeneID:144115270"
ORIGIN      
        1 mlppsdlahr adlvcvllrg elapaalvva pagilrywpn iahedcsida ladlhgeecc
       61 sltnteplgc ivatttsslm lvtpstiegq ttllikplkt pqglltgigq qvssfifgtm
      121 paqlearyla kvlveptmee ggkyfyvllr nslqkwflkd kdpeklcgec dldrcmkeqf
      181 hetvwngsnt lpmhlkawci dmqlpsngva ilmaalnphv sqrlyyaigt vdirslgspa
      241 drpasftdft vlrfqeqyte kdeeallsyk fvlpsamcps sfvysgtkvy cvmsgsdvgd
      301 ldviefkkdk llgagcyeet plffsrvngi vcirsvhata rdarsqlhes plhqsqvlee
      361 tanhvveifg telpvlvsea lieaepgtdi ttrisscgwa wlvcgrklcv wqykkglhnr
      421 nchvlmlpps dlahradlvc vllrgqlapa alvvapagil rywpniahed csidaladlh
      481 geeccsltnt eplgcivatt tsslmlvtps tiegqttlli kplktpqgll tgigqqvssf
      541 ifgtmpaqle arylakvlve ptmeeggkyf yvllrnslqk wflkdkdpek lcgecdldrc
      601 mkeqfhetvw ngsntlpmhl kawcidmqlp sngvailmaa lnphvsqrly yaigtvdvrs
      661 lgspadcpas ftdftvlrfq eqytekdeea llsykfvlps amcpssfvys gtkvycvmsg
      721 sdvgdldvie fkkdkllgag cyeetplffs rvngivcirs vhatardasl amelmpecpy
      781 sladdetmeg isvdernlhk lrsafalfcq nellksqeiv nnifprpvel frdldsaldt
      841 cvsqlgywlm dgvpvmdprw tqlqfpggsv tvssasllih rqledklqah qlflnflkav
      901 hlwprlyavt vrgspmatnl llqeqaeklv aamelhslqa qfntiidagi rrviqecsas
      961 tpggltaady fyrkvseldk ifpalveeee dqlkmglspr defalitnvn tifvwvlrdv
     1021 clfrdreqlt yecaretsle frpwttilrd iickqhtlat tnaiavaena tsfgqvflql
     1081 tdladlilng hkvqldslth nqaayealkl qyqqdrrhfi aplikatqye raatlaekhc
     1141 dfeslveice ltrnkerles ymvlfkdqgf pefvfqwqld sgrrgelldq pasqhgnler
     1201 fleghdslsw lhdihlgkmg kaattlhrlg qqeeqnmlrk kyllshskla alcsddppet
     1261 hadlikninr eealityqes lpfavkeayc leprgmkvlg peeliemyis tanlnaneyd
     1321 ftkaleildf iedptraqel rmriwcaail rnkwenldtd nvegimkdrl ffriadlgvt
     1381 qgrnlaqylp plegllempd lqdlrsnhhf lfilgagyeh lqrmaaikts lhqa
//
LOCUS       XP_077505705            1345 aa            linear   INV 25-NOV-2025
DEFINITION  nuclear pore complex protein Nup133-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505705
VERSION     XP_077505705.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649579.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1345
                     /product="nuclear pore complex protein Nup133-like isoform
                     X3"
                     /calculated_mol_wt=151479
     Region          309..667
                     /region_name="Nucleoporin_N"
                     /note="Nup133 N terminal like; pfam08801"
                     /db_xref="CDD:400932"
     Region          836..1276
                     /region_name="Nucleoporin_C"
                     /note="Non-repetitive/WGA-negative nucleoporin C-terminal;
                     pfam03177"
                     /db_xref="CDD:427181"
     CDS             1..1345
                     /gene="LOC144115270"
                     /coded_by="XM_077649579.1:76..4113"
                     /db_xref="GeneID:144115270"
ORIGIN      
        1 mttllikplk tpqglltgig qqvssfifgt mpaqlearyl akvlveptme eggkyfyvll
       61 rnslqkwflk dkdpeklcge cdldrcmkeq fhetvwngsn tlpmhlkawc idmqlpsngv
      121 ailmaalnph vsqrlyyaig tvdirslgsp adrpasftdf tvlrfqeqyt ekdeeallsy
      181 kfvlpsamcp ssfvysgtkv ycvmsgsdvg dldviefkkd kllgagcyee tplffsrvng
      241 ivcirsvhat ardarsqlhe splhqsqvle etanhvveif gtelpvlvse alieaepgtd
      301 ittrisscgw awlvcgrklc vwqykkglhn rnchvlmlpp sdlahradlv cvllrgqlap
      361 aalvvapagi lrywpniahe dcsidaladl hgeeccsltn teplgcivat ttsslmlvtp
      421 stiegqttll ikplktpqgl ltgigqqvss fifgtmpaql earylakvlv eptmeeggky
      481 fyvllrnslq kwflkdkdpe klcgecdldr cmkeqfhetv wngsntlpmh lkawcidmql
      541 psngvailma alnphvsqrl yyaigtvdvr slgspadcpa sftdftvlrf qeqytekdee
      601 allsykfvlp samcpssfvy sgtkvycvms gsdvgdldvi efkkdkllga gcyeetplff
      661 srvngivcir svhatardas lamelmpecp ysladdetme gisvdernlh klrsafalfc
      721 qnellksqei vnnifprpve lfrdldsald tcvsqlgywl mdgvpvmdpr wtqlqfpggs
      781 vtvssaslli hrqledklqa hqlflnflka vhlwprlyav tvrgspmatn lllqeqaekl
      841 vaamelhslq aqfntiidag irrviqecsa stpggltaad yfyrkvseld kifpalveee
      901 edqlkmglsp rdefalitnv ntifvwvlrd vclfrdreql tyecaretsl efrpwttilr
      961 diickqhtla ttnaiavaen atsfgqvflq ltdladliln ghkvqldslt hnqaayealk
     1021 lqyqqdrrhf iaplikatqy eraatlaekh cdfeslveic eltrnkerle symvlfkdqg
     1081 fpefvfqwql dsgrrgelld qpasqhgnle rfleghdsls wlhdihlgkm gkaattlhrl
     1141 gqqeeqnmlr kkyllshskl aalcsddppe thadliknin reealityqe slpfavkeay
     1201 cleprgmkvl gpeeliemyi stanlnaney dftkaleild fiedptraqe lrmriwcaai
     1261 lrnkwenldt dnvegimkdr lffriadlgv tqgrnlaqyl pplegllemp dlqdlrsnhh
     1321 flfilgagye hlqrmaaikt slhqa
//
LOCUS       XP_077505706             890 aa            linear   INV 25-NOV-2025
DEFINITION  fatty acid CoA ligase Acsl3-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505706
VERSION     XP_077505706.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649580.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..890
                     /product="fatty acid CoA ligase Acsl3-like isoform X1"
                     /calculated_mol_wt=98252
     Region          56..696
                     /region_name="AFD_class_I"
                     /note="Adenylate forming domain, Class I superfamily;
                     cl17068"
                     /db_xref="CDD:473059"
     Site            order(261,264..269,271..272)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341228"
     Site            order(264,428..429,450..455,540,552,555,567,677)
                     /site_type="other"
                     /note="AMP binding site [chemical binding]"
                     /db_xref="CDD:341228"
     Site            order(264,305..306,360,362..363,366,428..429,450..455,540,
                     552,555,564..567,652)
                     /site_type="active"
                     /db_xref="CDD:341228"
     Site            order(305,362..363,366,428,564..566,637,652)
                     /site_type="other"
                     /note="CoA binding site [chemical binding]"
                     /db_xref="CDD:341228"
     CDS             1..890
                     /gene="LOC144115271"
                     /coded_by="XM_077649580.1:252..2924"
                     /db_xref="GeneID:144115271"
ORIGIN      
        1 mifdfllrlv vtvydvvtlp iyitiqkpwi rvrrqnvtwa gplsnksdap vvrytpdpmk
       61 svfkgmetid affrravekf ddkpcfgtrv ckkdqcqivd gkrfvkyelg eyswktfkea
      121 daavdkiarg ltalghlpys rlvifaetre ewmltalacf rraivvctiy atlgdegivh
      181 ginetevktv itseellqrl aalvprlpnv thivymparg ttqpppptgs itvlpfdslt
      241 dskfsgdlpp vtptpqdvvi imytsgstgq pkgviltnsn vvafimgaas vlrefssgdt
      301 figllplahv meiavecsfm slgvkigyss pltltdqgpa lmpgvigdap llkptcmiav
      361 plllnriqkg veqavaakgp mvrnlfafam ayksywrkkg fntpilnrtv frnaknifgg
      421 rlraivsgsa plsddtqefl cncldcpvlq gygltettag atiqdkydla vgvagpplng
      481 vqiklvdwae ggyhvsdkpy prgevvvggp nvaagyfkrp eltaecfkdd gqtrwfftgd
      541 igeflpkgql riidrkkdlv klqygeyvsl gkvetvlkth plvenvcvyg spfstfvial
      601 vqpnesalrq laksvplpdn qpladlcddp lltnavtsdl vkhcrnkglv kfeiphkykl
      661 ckevwtpete lvtaalkirr iqiqkfyqkd idnmyhgvsn tgsaselhsp kpvsptadsd
      721 ttspktipal tekpmaqvep qvqtqsqeps qvpsqvqsqa qsqekssvps qahpevqale
      781 qpkvqpkvqp kvqpevqpqg qsqvqsqeqs qvqpqeqsqv qpqeqsqvqp qvqphvqpdv
      841 qaqeqpkvqt evqpeeqsqe qpqqqpqeqs qeqpqpqpqv qlqqqptave
//
LOCUS       XP_077505707             889 aa            linear   INV 25-NOV-2025
DEFINITION  fatty acid CoA ligase Acsl3-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505707
VERSION     XP_077505707.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649581.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..889
                     /product="fatty acid CoA ligase Acsl3-like isoform X2"
                     /calculated_mol_wt=98165
     Region          56..695
                     /region_name="AFD_class_I"
                     /note="Adenylate forming domain, Class I superfamily;
                     cl17068"
                     /db_xref="CDD:473059"
     Site            order(261,264..269,271..272)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341228"
     Site            order(264,427..428,449..454,539,551,554,566,676)
                     /site_type="other"
                     /note="AMP binding site [chemical binding]"
                     /db_xref="CDD:341228"
     Site            order(264,304..305,359,361..362,365,427..428,449..454,539,
                     551,554,563..566,651)
                     /site_type="active"
                     /db_xref="CDD:341228"
     Site            order(304,361..362,365,427,563..565,636,651)
                     /site_type="other"
                     /note="CoA binding site [chemical binding]"
                     /db_xref="CDD:341228"
     CDS             1..889
                     /gene="LOC144115271"
                     /coded_by="XM_077649581.1:252..2921"
                     /db_xref="GeneID:144115271"
ORIGIN      
        1 mifdfllrlv vtvydvvtlp iyitiqkpwi rvrrqnvtwa gplsnksdap vvrytpdpmk
       61 svfkgmetid affrravekf ddkpcfgtrv ckkdqcqivd gkrfvkyelg eyswktfkea
      121 daavdkiarg ltalghlpys rlvifaetre ewmltalacf rraivvctiy atlgdegivh
      181 ginetevktv itseellqrl aalvprlpnv thivymparg ttqpppptgs itvlpfdslt
      241 dskfsgdlpp vtptpqdvvi imytsgstgq pkgviltnsn vvafimgaas vlrefsgdtf
      301 igllplahvm eiavecsfms lgvkigyssp ltltdqgpal mpgvigdapl lkptcmiavp
      361 lllnriqkgv eqavaakgpm vrnlfafama yksywrkkgf ntpilnrtvf rnaknifggr
      421 lraivsgsap lsddtqeflc ncldcpvlqg ygltettaga tiqdkydlav gvagpplngv
      481 qiklvdwaeg gyhvsdkpyp rgevvvggpn vaagyfkrpe ltaecfkddg qtrwfftgdi
      541 geflpkgqlr iidrkkdlvk lqygeyvslg kvetvlkthp lvenvcvygs pfstfvialv
      601 qpnesalrql aksvplpdnq pladlcddpl ltnavtsdlv khcrnkglvk feiphkyklc
      661 kevwtpetel vtaalkirri qiqkfyqkdi dnmyhgvsnt gsaselhspk pvsptadsdt
      721 tspktipalt ekpmaqvepq vqtqsqepsq vpsqvqsqaq sqekssvpsq ahpevqaleq
      781 pkvqpkvqpk vqpevqpqgq sqvqsqeqsq vqpqeqsqvq pqeqsqvqpq vqphvqpdvq
      841 aqeqpkvqte vqpeeqsqeq pqqqpqeqsq eqpqpqpqvq lqqqptave
//
LOCUS       XP_077505708             517 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115272 [Amblyomma americanum].
ACCESSION   XP_077505708
VERSION     XP_077505708.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649582.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..517
                     /product="uncharacterized protein LOC144115272"
                     /calculated_mol_wt=58402
     CDS             1..517
                     /gene="LOC144115272"
                     /coded_by="XM_077649582.1:258..1811"
                     /db_xref="GeneID:144115272"
ORIGIN      
        1 mrsctsqddf rmkrglvssc idlfvsfgki aameleqris nwlpaldkei veltvqklqq
       61 cgveslehlk yvveedlqfl kpvsrrillq rfhsaatpnp pvslspndiq sptstqcasf
      121 stpwevfppq lmkacqegkr pvksdlnemv rlvsdhilai nrkpgrkilr aiaaeivshy
      181 pktfedtlng avigsgietl lwklencvnn krrpsseqph qfepddeldl svkrvkiqrd
      241 sygcvawqpk lpsdetadsq ekkkadllsq frlafpdetm vaqlmsetya sqrplinask
      301 nirdierswp flfqakhltn hvnillgsnv aktfddrlkt vgrqvfryah sqvkkecvks
      361 clqeieaakt nrksmaveye ampllllaif gedkelfykl teetasdddl gdlpscpfic
      421 vkgwtfltan syticvdthp vlhaskpdea fkllffahfa fniqyqkets lcleftqrai
      481 aginpargtk vqknggkqhc lsprvaalvt alkdydf
//
LOCUS       XP_077505709             123 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115273 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505709
VERSION     XP_077505709.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649583.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..123
                     /product="uncharacterized protein LOC144115273 isoform X1"
                     /calculated_mol_wt=12395
     CDS             1..123
                     /gene="LOC144115273"
                     /coded_by="XM_077649583.1:114..485"
                     /db_xref="GeneID:144115273"
ORIGIN      
        1 mhrgtsswla tgraqdgsar faggsaggaa rgvpglqral gaggpasepq araglllrav
       61 rqpgaargrr qarlqpaarp aaalgltgrd agplplrvtl lfllfllifl lacsttpmps
      121 rpp
//
LOCUS       XP_077505710             102 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115273 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505710
VERSION     XP_077505710.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649584.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..102
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..102
                     /product="uncharacterized protein LOC144115273 isoform X2"
                     /calculated_mol_wt=10941
     CDS             1..102
                     /gene="LOC144115273"
                     /coded_by="XM_077649584.1:121..429"
                     /db_xref="GeneID:144115273"
ORIGIN      
        1 mhrgtsswla tvvmlcplll lvagavaasg wpidraarna ddrdllamry lsggggsrpt
       61 wpnkralslf ahwrpqytpg gdepelpsdi sgrphglplr wg
//
LOCUS       XP_077505711              89 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115273 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505711
VERSION     XP_077505711.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649585.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..89
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..89
                     /product="uncharacterized protein LOC144115273 isoform X3"
                     /calculated_mol_wt=9514
     CDS             1..89
                     /gene="LOC144115273"
                     /coded_by="XM_077649585.1:147..416"
                     /db_xref="GeneID:144115273"
ORIGIN      
        1 mlcpllllva gavaasgwpi draarnaddr dllamrylsg gggsrptwpn kralslfahw
       61 rpqytpggde pelpsdisgr phglplrwg
//
LOCUS       XP_077505712              89 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115273 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505712
VERSION     XP_077505712.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649586.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..89
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..89
                     /product="uncharacterized protein LOC144115273 isoform X3"
                     /calculated_mol_wt=9514
     CDS             1..89
                     /gene="LOC144115273"
                     /coded_by="XM_077649586.1:530..799"
                     /db_xref="GeneID:144115273"
ORIGIN      
        1 mlcpllllva gavaasgwpi draarnaddr dllamrylsg gggsrptwpn kralslfahw
       61 rpqytpggde pelpsdisgr phglplrwg
//
LOCUS       XP_077505713              89 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115273 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505713
VERSION     XP_077505713.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649587.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..89
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..89
                     /product="uncharacterized protein LOC144115273 isoform X3"
                     /calculated_mol_wt=9514
     CDS             1..89
                     /gene="LOC144115273"
                     /coded_by="XM_077649587.1:695..964"
                     /db_xref="GeneID:144115273"
ORIGIN      
        1 mlcpllllva gavaasgwpi draarnaddr dllamrylsg gggsrptwpn kralslfahw
       61 rpqytpggde pelpsdisgr phglplrwg
//
LOCUS       XP_077505714              89 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115273 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505714
VERSION     XP_077505714.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649588.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..89
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..89
                     /product="uncharacterized protein LOC144115273 isoform X3"
                     /calculated_mol_wt=9514
     CDS             1..89
                     /gene="LOC144115273"
                     /coded_by="XM_077649588.1:311..580"
                     /db_xref="GeneID:144115273"
ORIGIN      
        1 mlcpllllva gavaasgwpi draarnaddr dllamrylsg gggsrptwpn kralslfahw
       61 rpqytpggde pelpsdisgr phglplrwg
//
LOCUS       XP_077505715              89 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115273 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505715
VERSION     XP_077505715.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649589.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..89
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..89
                     /product="uncharacterized protein LOC144115273 isoform X3"
                     /calculated_mol_wt=9514
     CDS             1..89
                     /gene="LOC144115273"
                     /coded_by="XM_077649589.1:533..802"
                     /db_xref="GeneID:144115273"
ORIGIN      
        1 mlcpllllva gavaasgwpi draarnaddr dllamrylsg gggsrptwpn kralslfahw
       61 rpqytpggde pelpsdisgr phglplrwg
//
LOCUS       XP_077505716              89 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115273 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505716
VERSION     XP_077505716.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649590.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..89
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..89
                     /product="uncharacterized protein LOC144115273 isoform X3"
                     /calculated_mol_wt=9514
     CDS             1..89
                     /gene="LOC144115273"
                     /coded_by="XM_077649590.1:705..974"
                     /db_xref="GeneID:144115273"
ORIGIN      
        1 mlcpllllva gavaasgwpi draarnaddr dllamrylsg gggsrptwpn kralslfahw
       61 rpqytpggde pelpsdisgr phglplrwg
//
LOCUS       XP_077505717            1409 aa            linear   INV 25-NOV-2025
DEFINITION  tyrosine-protein kinase Abl isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505717
VERSION     XP_077505717.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649591.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1409
                     /product="tyrosine-protein kinase Abl isoform X1"
                     /calculated_mol_wt=154145
     Region          113..167
                     /region_name="SH3_Abl"
                     /note="Src homology 3 domain of the Protein Tyrosine
                     Kinase, Abelson kinase; cd11850"
                     /db_xref="CDD:212784"
     Site            order(118,120,123..125,127,142,146..147,159,161,163..164)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212784"
     Site            order(122..125,144,146)
                     /site_type="other"
                     /note="PTKc domain interface [polypeptide binding]"
                     /db_xref="CDD:212784"
     Region          172..265
                     /region_name="SH2_ABL"
                     /note="Src homology 2 (SH2) domain found in Abelson murine
                     lymphosarcoma virus (ABL) proteins; cd09935"
                     /db_xref="CDD:198189"
     Site            order(183,211,224)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198189"
     Region          284..546
                     /region_name="PTKc_Abl"
                     /note="Catalytic domain of the Protein Tyrosine Kinase,
                     Abelson kinase; cd05052"
                     /db_xref="CDD:270645"
     Site            order(297,299..301,318,320,365,367,371,419,430,448..450,
                     452,460,494,498)
                     /site_type="active"
                     /db_xref="CDD:270645"
     Site            order(297,299,302,318,320,335,339,348,364..365,367,371,
                     419,429..430)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270645"
     Site            order(312..313,343..345,349,391,423..424,542..543)
                     /site_type="other"
                     /note="SH3/SH2 domain interface [polypeptide binding]"
                     /db_xref="CDD:270645"
     Site            order(389..390,393,478,481..482,511..512)
                     /site_type="other"
                     /note="myristoyl binding site [chemical binding]"
                     /db_xref="CDD:270645"
     Site            429..453
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270645"
     Site            order(448..450,452,460,494,498)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270645"
     Region          1302..1409
                     /region_name="F_actin_bind"
                     /note="F-actin binding; pfam08919"
                     /db_xref="CDD:462633"
     CDS             1..1409
                     /gene="Abl"
                     /coded_by="XM_077649591.1:141..4370"
                     /db_xref="GeneID:144115275"
ORIGIN      
        1 mgaqhgkenr gsassvgsss hgnkltkhgq kqkakdirls svsanifteh sgpslqctqv
       61 yvvsaeallq srplpeipdg ddpgsfrgfl aegasrwmsk enllaqedad phlfvalydf
      121 qsggenqlsl kkgeqvkvvs ynrtgewcea qgrsgqvgwv psnyvtpvns lekhswyhgp
      181 iarnaaeyll ssgingsflv resesspgqr sislrcegrv yhyrinedse gkvyvtsdwr
      241 fntlaelvhh hslhadglit qllypapkrn kpavfalspe pdeweidrtd ivmkhrlggg
      301 qygdvyealw krynltvavk tlkedtmalk dfleeaaimk emkhpnlvql igvctreppf
      361 yiitefmahg nlleylrncs reeitavvlm ymatqiasam sylearsfih rdlaarnclv
      421 gdchlvkvad fglarlmrdd tytahagakf pikwtapegl aynkfstksd vwafgillwe
      481 iatygmspyp gvdltdvyhm lesgyrmecp pgcpvrvyel mrqcwqwett drptfqeihn
      541 tlenmfqnss iteeverqle kqspamaykk hpgssptigt mtdddqenra pdtsnqavvp
      601 tkasqgllqv kastvqlrrp gprskqapvp pkrtssfrds tyqdrnpdde dgdeeelnng
      661 glervfetag dcsgpeakqs tpdteeselh sttsvpdvsq qllntkpqkl rkartyppnl
      721 qpqrtgkdas valstkqakk vqvaalevqn vkrainrygt lpkgarigay leslrqhglh
      781 tgtsyqepvv edghvhyqsg nvlsvgsgar mavgadygha rckigeaapp hailqrqksd
      841 lthtkvsdaf etgsipemrr pgpkqgpspr larhhrfdgk sttpgrdmps dglqdavses
      901 eaqlgvsqkr esfeslalqs qegkavpatv spvalfgssv vrrtlkrrpk drppsppkhp
      961 lvksgsvddq lsrhsgnsna atkaasdsnq spvmpeapdg spisysdegf ppppefsnpd
     1021 qqtlecqdvh ssplaeastr ssaaqvvsel fenlkmkarr kaaetgeaca qttgdpcrga
     1081 sppeknatdv pakpatsqkk alveevkkre pdrlqsptqp ldkkkapvee vkkrepdrlq
     1141 sptqpldlpa qgeeekrqss gsisslkklw eketgggkvs pkigarrpes lrlskneggd
     1201 grkasppspp stvvpprtpd dapdsqqqqq qftskpvvpt kpalktaskl psstankfak
     1261 sasssssssa pnqpkppvvp rnqsstspqp kkgaqqdedr ladskssile issalesgiv
     1321 alkaavpgsl ssvsvmqlsd kvqllcsscs syaesvpphg rfrfrellsr letqgdqlrt
     1381 cssnnsaasa klftdlhntv rdlvnvvqr
//
LOCUS       XP_077505718            1395 aa            linear   INV 25-NOV-2025
DEFINITION  tyrosine-protein kinase Abl isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505718
VERSION     XP_077505718.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649592.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1395
                     /product="tyrosine-protein kinase Abl isoform X2"
                     /calculated_mol_wt=152712
     Region          99..153
                     /region_name="SH3_Abl"
                     /note="Src homology 3 domain of the Protein Tyrosine
                     Kinase, Abelson kinase; cd11850"
                     /db_xref="CDD:212784"
     Site            order(104,106,109..111,113,128,132..133,145,147,149..150)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212784"
     Site            order(108..111,130,132)
                     /site_type="other"
                     /note="PTKc domain interface [polypeptide binding]"
                     /db_xref="CDD:212784"
     Region          158..251
                     /region_name="SH2_ABL"
                     /note="Src homology 2 (SH2) domain found in Abelson murine
                     lymphosarcoma virus (ABL) proteins; cd09935"
                     /db_xref="CDD:198189"
     Site            order(169,197,210)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198189"
     Region          270..532
                     /region_name="PTKc_Abl"
                     /note="Catalytic domain of the Protein Tyrosine Kinase,
                     Abelson kinase; cd05052"
                     /db_xref="CDD:270645"
     Site            order(283,285..287,304,306,351,353,357,405,416,434..436,
                     438,446,480,484)
                     /site_type="active"
                     /db_xref="CDD:270645"
     Site            order(283,285,288,304,306,321,325,334,350..351,353,357,
                     405,415..416)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270645"
     Site            order(298..299,329..331,335,377,409..410,528..529)
                     /site_type="other"
                     /note="SH3/SH2 domain interface [polypeptide binding]"
                     /db_xref="CDD:270645"
     Site            order(375..376,379,464,467..468,497..498)
                     /site_type="other"
                     /note="myristoyl binding site [chemical binding]"
                     /db_xref="CDD:270645"
     Site            415..439
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270645"
     Site            order(434..436,438,446,480,484)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270645"
     Region          1288..1395
                     /region_name="F_actin_bind"
                     /note="F-actin binding; pfam08919"
                     /db_xref="CDD:462633"
     CDS             1..1395
                     /gene="Abl"
                     /coded_by="XM_077649592.1:141..4328"
                     /db_xref="GeneID:144115275"
ORIGIN      
        1 mgaqhgkenr gsassvgsss hgnkltkhgq kqkakdirls svsanifteh seallqsrpl
       61 peipdgddpg sfrgflaega srwmskenll aqedadphlf valydfqsgg enqlslkkge
      121 qvkvvsynrt gewceaqgrs gqvgwvpsny vtpvnslekh swyhgpiarn aaeyllssgi
      181 ngsflvrese sspgqrsisl rcegrvyhyr inedsegkvy vtsdwrfntl aelvhhhslh
      241 adglitqlly papkrnkpav falspepdew eidrtdivmk hrlgggqygd vyealwkryn
      301 ltvavktlke dtmalkdfle eaaimkemkh pnlvqligvc treppfyiit efmahgnlle
      361 ylrncsreei tavvlmymat qiasamsyle arsfihrdla arnclvgdch lvkvadfgla
      421 rlmrddtyta hagakfpikw tapeglaynk fstksdvwaf gillweiaty gmspypgvdl
      481 tdvyhmlesg yrmecppgcp vrvyelmrqc wqwettdrpt fqeihntlen mfqnssitee
      541 verqlekqsp amaykkhpgs sptigtmtdd dqenrapdts nqavvptkas qgllqvkast
      601 vqlrrpgprs kqapvppkrt ssfrdstyqd rnpddedgde eelnnggler vfetagdcsg
      661 peakqstpdt eeselhstts vpdvsqqlln tkpqklrkar typpnlqpqr tgkdasvals
      721 tkqakkvqva alevqnvkra inrygtlpkg arigaylesl rqhglhtgts yqepvvedgh
      781 vhyqsgnvls vgsgarmavg adygharcki geaapphail qrqksdltht kvsdafetgs
      841 ipemrrpgpk qgpsprlarh hrfdgksttp grdmpsdglq davseseaql gvsqkresfe
      901 slalqsqegk avpatvspva lfgssvvrrt lkrrpkdrpp sppkhplvks gsvddqlsrh
      961 sgnsnaatka asdsnqspvm peapdgspis ysdegfpppp efsnpdqqtl ecqdvhsspl
     1021 aeastrssaa qvvselfenl kmkarrkaae tgeacaqttg dpcrgasppe knatdvpakp
     1081 atsqkkalve evkkrepdrl qsptqpldkk kapveevkkr epdrlqsptq pldlpaqgee
     1141 ekrqssgsis slkklweket gggkvspkig arrpeslrls kneggdgrka sppsppstvv
     1201 pprtpddapd sqqqqqqfts kpvvptkpal ktasklpsst ankfaksass sssssapnqp
     1261 kppvvprnqs stspqpkkga qqdedrlads kssileissa lesgivalka avpgslssvs
     1321 vmqlsdkvql lcsscssyae svpphgrfrf rellsrletq gdqlrtcssn nsaasaklft
     1381 dlhntvrdlv nvvqr
//
LOCUS       XP_077505719            1358 aa            linear   INV 25-NOV-2025
DEFINITION  tyrosine-protein kinase Abl isoform X3 [Amblyomma americanum].
ACCESSION   XP_077505719
VERSION     XP_077505719.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649593.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1358
                     /product="tyrosine-protein kinase Abl isoform X3"
                     /calculated_mol_wt=148260
     Region          113..167
                     /region_name="SH3_Abl"
                     /note="Src homology 3 domain of the Protein Tyrosine
                     Kinase, Abelson kinase; cd11850"
                     /db_xref="CDD:212784"
     Site            order(118,120,123..125,127,142,146..147,159,161,163..164)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212784"
     Site            order(122..125,144,146)
                     /site_type="other"
                     /note="PTKc domain interface [polypeptide binding]"
                     /db_xref="CDD:212784"
     Region          172..265
                     /region_name="SH2_ABL"
                     /note="Src homology 2 (SH2) domain found in Abelson murine
                     lymphosarcoma virus (ABL) proteins; cd09935"
                     /db_xref="CDD:198189"
     Site            order(183,211,224)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198189"
     Region          284..495
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(297..300,303,305,318,320,348,364..367,412,416..417,
                     419,429..430)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     Region          1251..1358
                     /region_name="F_actin_bind"
                     /note="F-actin binding; pfam08919"
                     /db_xref="CDD:462633"
     CDS             1..1358
                     /gene="Abl"
                     /coded_by="XM_077649593.1:141..4217"
                     /db_xref="GeneID:144115275"
ORIGIN      
        1 mgaqhgkenr gsassvgsss hgnkltkhgq kqkakdirls svsanifteh sgpslqctqv
       61 yvvsaeallq srplpeipdg ddpgsfrgfl aegasrwmsk enllaqedad phlfvalydf
      121 qsggenqlsl kkgeqvkvvs ynrtgewcea qgrsgqvgwv psnyvtpvns lekhswyhgp
      181 iarnaaeyll ssgingsflv resesspgqr sislrcegrv yhyrinedse gkvyvtsdwr
      241 fntlaelvhh hslhadglit qllypapkrn kpavfalspe pdeweidrtd ivmkhrlggg
      301 qygdvyealw krynltvavk tlkedtmalk dfleeaaimk emkhpnlvql igvctreppf
      361 yiitefmahg nlleylrncs reeitavvlm ymatqiasam sylearsfih rdlaarnclv
      421 gdchlvkvad fglarlmrdd tytahagakf pikwtapegl aynkfstksd vwgwqwettd
      481 rptfqeihnt lenmfqnssi teeverqlek qspamaykkh pgssptigtm tdddqenrap
      541 dtsnqavvpt kasqgllqvk astvqlrrpg prskqapvpp krtssfrdst yqdrnpdded
      601 gdeeelnngg lervfetagd csgpeakqst pdteeselhs ttsvpdvsqq llntkpqklr
      661 kartyppnlq pqrtgkdasv alstkqakkv qvaalevqnv krainrygtl pkgarigayl
      721 eslrqhglht gtsyqepvve dghvhyqsgn vlsvgsgarm avgadyghar ckigeaapph
      781 ailqrqksdl thtkvsdafe tgsipemrrp gpkqgpsprl arhhrfdgks ttpgrdmpsd
      841 glqdavsese aqlgvsqkre sfeslalqsq egkavpatvs pvalfgssvv rrtlkrrpkd
      901 rppsppkhpl vksgsvddql srhsgnsnaa tkaasdsnqs pvmpeapdgs pisysdegfp
      961 pppefsnpdq qtlecqdvhs splaeastrs saaqvvself enlkmkarrk aaetgeacaq
     1021 ttgdpcrgas ppeknatdvp akpatsqkka lveevkkrep drlqsptqpl dkkkapveev
     1081 kkrepdrlqs ptqpldlpaq geeekrqssg sisslkklwe ketgggkvsp kigarrpesl
     1141 rlskneggdg rkasppspps tvvpprtpdd apdsqqqqqq ftskpvvptk palktasklp
     1201 sstankfaks asssssssap nqpkppvvpr nqsstspqpk kgaqqdedrl adskssilei
     1261 ssalesgiva lkaavpgsls svsvmqlsdk vqllcsscss yaesvpphgr frfrellsrl
     1321 etqgdqlrtc ssnnsaasak lftdlhntvr dlvnvvqr
//
LOCUS       XP_077505720            1261 aa            linear   INV 25-NOV-2025
DEFINITION  tyrosine-protein kinase Abl isoform X4 [Amblyomma americanum].
ACCESSION   XP_077505720
VERSION     XP_077505720.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649594.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1261
                     /product="tyrosine-protein kinase Abl isoform X4"
                     /calculated_mol_wt=138001
     Region          16..70
                     /region_name="SH3_Abl"
                     /note="Src homology 3 domain of the Protein Tyrosine
                     Kinase, Abelson kinase; cd11850"
                     /db_xref="CDD:212784"
     Site            order(21,23,26..28,30,45,49..50,62,64,66..67)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212784"
     Site            order(25..28,47,49)
                     /site_type="other"
                     /note="PTKc domain interface [polypeptide binding]"
                     /db_xref="CDD:212784"
     Region          75..168
                     /region_name="SH2_ABL"
                     /note="Src homology 2 (SH2) domain found in Abelson murine
                     lymphosarcoma virus (ABL) proteins; cd09935"
                     /db_xref="CDD:198189"
     Site            order(86,114,127)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198189"
     Region          187..398
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(200..203,206,208,221,223,251,267..270,315,319..320,
                     322,332..333)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     Region          1154..1261
                     /region_name="F_actin_bind"
                     /note="F-actin binding; pfam08919"
                     /db_xref="CDD:462633"
     CDS             1..1261
                     /gene="Abl"
                     /coded_by="XM_077649594.1:435..4220"
                     /db_xref="GeneID:144115275"
ORIGIN      
        1 mskenllaqe dadphlfval ydfqsggenq lslkkgeqvk vvsynrtgew ceaqgrsgqv
       61 gwvpsnyvtp vnslekhswy hgpiarnaae yllssgings flvresessp gqrsislrce
      121 grvyhyrine dsegkvyvts dwrfntlael vhhhslhadg litqllypap krnkpavfal
      181 spepdeweid rtdivmkhrl gggqygdvye alwkrynltv avktlkedtm alkdfleeaa
      241 imkemkhpnl vqligvctre ppfyiitefm ahgnlleylr ncsreeitav vlmymatqia
      301 samsylears fihrdlaarn clvgdchlvk vadfglarlm rddtytahag akfpikwtap
      361 eglaynkfst ksdvwgwqwe ttdrptfqei hntlenmfqn ssiteeverq lekqspamay
      421 kkhpgsspti gtmtdddqen rapdtsnqav vptkasqgll qvkastvqlr rpgprskqap
      481 vppkrtssfr dstyqdrnpd dedgdeeeln ngglervfet agdcsgpeak qstpdteese
      541 lhsttsvpdv sqqllntkpq klrkartypp nlqpqrtgkd asvalstkqa kkvqvaalev
      601 qnvkrainry gtlpkgarig ayleslrqhg lhtgtsyqep vvedghvhyq sgnvlsvgsg
      661 armavgadyg harckigeaa pphailqrqk sdlthtkvsd afetgsipem rrpgpkqgps
      721 prlarhhrfd gksttpgrdm psdglqdavs eseaqlgvsq kresfeslal qsqegkavpa
      781 tvspvalfgs svvrrtlkrr pkdrppsppk hplvksgsvd dqlsrhsgns naatkaasds
      841 nqspvmpeap dgspisysde gfppppefsn pdqqtlecqd vhssplaeas trssaaqvvs
      901 elfenlkmka rrkaaetgea caqttgdpcr gasppeknat dvpakpatsq kkalveevkk
      961 repdrlqspt qpldkkkapv eevkkrepdr lqsptqpldl paqgeeekrq ssgsisslkk
     1021 lweketgggk vspkigarrp eslrlskneg gdgrkaspps ppstvvpprt pddapdsqqq
     1081 qqqftskpvv ptkpalktas klpsstankf aksassssss sapnqpkppv vprnqsstsp
     1141 qpkkgaqqde drladskssi leissalesg ivalkaavpg slssvsvmql sdkvqllcss
     1201 cssyaesvpp hgrfrfrell srletqgdql rtcssnnsaa saklftdlhn tvrdlvnvvq
     1261 r
//
LOCUS       XP_077505721             115 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115276 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505721
VERSION     XP_077505721.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649595.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..115
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..115
                     /product="uncharacterized protein LOC144115276 isoform X1"
                     /calculated_mol_wt=12552
     CDS             1..115
                     /gene="LOC144115276"
                     /coded_by="XM_077649595.1:1330..1677"
                     /db_xref="GeneID:144115276"
ORIGIN      
        1 mcdsfgqkat aagqshpprf ynfvtgpshn enlerlarkh rwrgrllrqr kpasactgar
       61 tpaaftwstr nqgqapaact stedgkvhma ngvhisgeay ttlmvtqkss mfckk
//
LOCUS       XP_077505722              75 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115276 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505722
VERSION     XP_077505722.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649596.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..75
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..75
                     /product="uncharacterized protein LOC144115276 isoform X2"
                     /calculated_mol_wt=8003
     CDS             1..75
                     /gene="LOC144115276"
                     /coded_by="XM_077649596.1:1297..1524"
                     /db_xref="GeneID:144115276"
ORIGIN      
        1 mcdsfgqkat aagqshpprf ynfvttwstr nqgqapaact stedgkvhma ngvhisgeay
       61 ttlmvtqkss mfckk
//
LOCUS       XP_077505723             115 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115279 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505723
VERSION     XP_077505723.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649597.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..115
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..115
                     /product="uncharacterized protein LOC144115279 isoform X1"
                     /calculated_mol_wt=12552
     CDS             1..115
                     /gene="LOC144115279"
                     /coded_by="XM_077649597.1:1329..1676"
                     /db_xref="GeneID:144115279"
ORIGIN      
        1 mcdsfgqkat aagqshpprf ynfvtgpshn enlerlarkh rwrgrllrqr kpasactgar
       61 tpaaftwstr nqgqapaact stedgkvhma ngvhisgeay ttlmvtqkss mfckk
//
LOCUS       XP_077505724              75 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115279 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505724
VERSION     XP_077505724.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649598.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..75
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..75
                     /product="uncharacterized protein LOC144115279 isoform X2"
                     /calculated_mol_wt=8003
     CDS             1..75
                     /gene="LOC144115279"
                     /coded_by="XM_077649598.1:1296..1523"
                     /db_xref="GeneID:144115279"
ORIGIN      
        1 mcdsfgqkat aagqshpprf ynfvttwstr nqgqapaact stedgkvhma ngvhisgeay
       61 ttlmvtqkss mfckk
//
LOCUS       XP_077505726            1072 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: calcium-activated potassium channel
            slowpoke-like [Amblyomma americanum].
ACCESSION   XP_077505726
VERSION     XP_077505726.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649600.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 3 indels
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1072
                     /product="LOW QUALITY PROTEIN: calcium-activated potassium
                     channel slowpoke-like"
                     /calculated_mol_wt=118964
     Region          30..213
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          <165..>274
                     /region_name="PRK10537"
                     /note="voltage-gated potassium channel protein"
                     /db_xref="CDD:236711"
     Region          368..456
                     /region_name="BK_channel_a"
                     /note="Calcium-activated BK potassium channel alpha
                     subunit; pfam03493"
                     /db_xref="CDD:460947"
     CDS             1..1072
                     /gene="LOC144115280"
                     /coded_by="XM_077649600.1:2445..5663"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 3 bases in 3 codons"
                     /db_xref="GeneID:144115280"
ORIGIN      
        1 manltfclft aavlvaflrf ssrdgvekcq swstnttqqi dlalniffmv yffirfiaas
       61 dklwfmlely sxvdyftipx sfvsiyldrt wiglrflral rmmsvpdilq ylnvlktsss
      121 irlaqlvsiv isvwltaagi ihllensgdp ldfanarklt ywecvyyliv tmstvgygdv
      181 ycqttlgqvf ivlfilvgla vfascipeil eligtrpkyg geyrrehgkr hivvcghity
      241 esvshflkdf lhedredvdv evvflhrkpp dleleglikr hfttveffqg svmnpidlqr
      301 vkvheadacl vlankycqdp daedaanimr visiknysdd irviiqlmqy hnkayllnip
      361 swnwkrgddv icvselklgf iaqsclapgf stmmanlfam rsyktspdmp awqndylcgt
      421 gmemytesls asftgmtfpq aaelcfvklk llllaievav ddgadskiai npkkkikitl
      481 ntqgffiaqs adevkrawfy cknchedikd eklikkckck npagvglfkk gvravqavsr
      541 aneglhnsnv ptftpteipk rvklrveees ftytatqpqv gnnndqnrvs sapkpkpstk
      601 khdqsnslts pnerggrsap psyaspvsdt shvtrtaapd mfaglhlaye vkklmptsrg
      661 tngtgnsqne kpfpvglsdd qakdfektem kydstgmfhw csarpiedci ldrnqaamtv
      721 lnghvvvclf adpdspligl rnlvmplras nfhyhelkhv vivgnvdylr rewkmlqnlp
      781 kisvlngspl sradlravni nlcdmcvils akipssddpt ladkeailas lnikamtfdd
      841 tigvlthnpn gaegfsplgs pivmqrrgsv yganvplite lvndtnvqfl dqdddddpdt
      901 elyltqpfac gtafavsvld slmsttyfna naltlirsli tggatpelel ilaegaglrg
      961 gystpeslan rdxcrvgqis lydgplaqfg eggkygdlfv aalrsygmlc vglyrfrdts
     1021 ssgeasskry vitnppadft llptdmvfvl lqfdpgleyk pnraeepkdd ns
//
LOCUS       XP_077505727             131 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115281 [Amblyomma americanum].
ACCESSION   XP_077505727
VERSION     XP_077505727.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649601.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..131
                     /product="uncharacterized protein LOC144115281"
                     /calculated_mol_wt=14971
     Region          <80..116
                     /region_name="GT1"
                     /note="myb-like, SANT family; cl23759"
                     /db_xref="CDD:474047"
     CDS             1..131
                     /gene="LOC144115281"
                     /coded_by="XM_077649601.1:3940..4335"
                     /db_xref="GeneID:144115281"
ORIGIN      
        1 mermsslplk vhltthqthn taitgasclw frdeegyvyn tpkgipkdav flkrasstiw
       61 sreavaqcsf igdvktgtlr wrnlrdtfck kvqelkkkss swlyfeqmmf lrdiieprgs
      121 gsenlqhlsa m
//
LOCUS       XP_077505728             126 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115282 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505728
VERSION     XP_077505728.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649602.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..126
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..126
                     /product="uncharacterized protein LOC144115282 isoform X1"
                     /calculated_mol_wt=13618
     CDS             1..126
                     /gene="LOC144115282"
                     /coded_by="XM_077649602.1:75..455"
                     /db_xref="GeneID:144115282"
ORIGIN      
        1 mpwhctidep rrradensap ggsgtltgqs gllycldtgc ihllfrdgeg yirtldriqk
       61 davflkratt tvlsreaasl vtaqegdsrr qgggfvegtq vkidaltgiv dthswiqdai
      121 kamkdi
//
LOCUS       XP_077505729             115 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115282 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505729
VERSION     XP_077505729.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649603.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..115
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..115
                     /product="uncharacterized protein LOC144115282 isoform X2"
                     /calculated_mol_wt=13147
     CDS             1..115
                     /gene="LOC144115282"
                     /coded_by="XM_077649603.1:352..699"
                     /db_xref="GeneID:144115282"
ORIGIN      
        1 msvtvrstsl vveptrirhl aavglspdnp dcyiawtqga fiycfemeka iyapwteskr
       61 mqyfskgppq lscqekqlhw mrllqvicll rtshlvkvih alwihtagfk mpskq
//
LOCUS       XP_077505730             427 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115286 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505730
VERSION     XP_077505730.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649604.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..427
                     /product="uncharacterized protein LOC144115286 isoform X1"
                     /calculated_mol_wt=45383
     Region          10..90
                     /region_name="PDZ_ZASP52-like"
                     /note="PDZ domain of Drosophila melanogaster PDZ and LIM
                     domain protein Zasp52 (also known as Zasp), and related
                     domains; cd23068"
                     /db_xref="CDD:467281"
     Site            order(21..27,39,42,70,74,77..78)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467281"
     Region          280..>365
                     /region_name="DUF4749"
                     /note="Domain of unknown function (DUF4749); pfam15936"
                     /db_xref="CDD:464948"
     Region          280..305
                     /region_name="ZM"
                     /note="ZASP-like motif; smart00735"
                     /db_xref="CDD:128974"
     CDS             1..427
                     /gene="LOC144115286"
                     /coded_by="XM_077649604.1:281..1564"
                     /db_xref="GeneID:144115286"
ORIGIN      
        1 msyqkggvls vglrrdfsat pwgfrlqggf dlnapltiqr vflgspsege lhrgdlvlki
       61 gdrdtsrmth neatelirns gnnlslvihr spgphspast pttpkapgma agghplagat
      121 yrpagppayv spqspgitit tqnvsvpapg pyqdgqyqye lrqgflvpep lvenkidalk
      181 eqakitnqty rttplitpsa kprrdvtigs ylrhvkepyf ttmkmpvdsl nnpdaiakkv
      241 qetvnqvasg snspcfglpa svavsggsgg rstpgadgpk ivhnvynspi hlystqnvad
      301 tlaaqagvkl gqgnlqpsgs aspqqqqhsp rsvgsagsgv gmarpgtkla saaditlspt
      361 yqmihgeewr dirksdlqeh rpvqnqlyav qpdygnaina fgapkgriqq snsfktimst
      421 vmtpkll
//
LOCUS       XP_077505732             420 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115286 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505732
VERSION     XP_077505732.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649606.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..420
                     /product="uncharacterized protein LOC144115286 isoform X2"
                     /calculated_mol_wt=44800
     Region          <31..83
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cl49608"
                     /db_xref="CDD:483948"
     Region          273..>358
                     /region_name="DUF4749"
                     /note="Domain of unknown function (DUF4749); pfam15936"
                     /db_xref="CDD:464948"
     Region          273..298
                     /region_name="ZM"
                     /note="ZASP-like motif; smart00735"
                     /db_xref="CDD:128974"
     CDS             1..420
                     /gene="LOC144115286"
                     /coded_by="XM_077649606.1:582..1844"
                     /db_xref="GeneID:144115286"
ORIGIN      
        1 mlkatekpdc lkdardyrry taapkkktkd vsevflgsps egelhrgdlv lkigdrdtsr
       61 mthneateli rnsgnnlslv ihrspgphsp astpttpkap gmaagghpla gatyrpagpp
      121 ayvspqspgi tittqnvsvp apgpyqdgqy qyelrqgflv peplvenkid alkeqakitn
      181 qtyrttplit psakprrdvt igsylrhvke pyfttmkmpv dslnnpdaia kkvqetvnqv
      241 asgsnspcfg lpasvavsgg sggrstpgad gpkivhnvyn spihlystqn vadtlaaqag
      301 vklgqgnlqp sgsaspqqqq hsprsvgsag sgvgmarpgt klasaaditl sptyqmihge
      361 ewrdirksdl qehrpvqnql yavqpdygna inafgapkgr iqqsnsfkti mstvmtpkll
//
LOCUS       XP_077505733             417 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115286 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505733
VERSION     XP_077505733.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649607.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..417
                     /product="uncharacterized protein LOC144115286 isoform X3"
                     /calculated_mol_wt=44484
     Region          <31..80
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cl49608"
                     /db_xref="CDD:483948"
     Region          270..>355
                     /region_name="DUF4749"
                     /note="Domain of unknown function (DUF4749); pfam15936"
                     /db_xref="CDD:464948"
     Region          270..295
                     /region_name="ZM"
                     /note="ZASP-like motif; smart00735"
                     /db_xref="CDD:128974"
     CDS             1..417
                     /gene="LOC144115286"
                     /coded_by="XM_077649607.1:146..1399"
                     /db_xref="GeneID:144115286"
ORIGIN      
        1 mlkatekpdc lkdardyrry taapkkktkd vflgspsege lhrgdlvlki gdrdtsrmth
       61 neatelirns gnnlslvihr spgphspast pttpkapgma agghplagat yrpagppayv
      121 spqspgitit tqnvsvpapg pyqdgqyqye lrqgflvpep lvenkidalk eqakitnqty
      181 rttplitpsa kprrdvtigs ylrhvkepyf ttmkmpvdsl nnpdaiakkv qetvnqvasg
      241 snspcfglpa svavsggsgg rstpgadgpk ivhnvynspi hlystqnvad tlaaqagvkl
      301 gqgnlqpsgs aspqqqqhsp rsvgsagsgv gmarpgtkla saaditlspt yqmihgeewr
      361 dirksdlqeh rpvqnqlyav qpdygnaina fgapkgriqq snsfktimst vmtpkll
//
LOCUS       XP_077505734             355 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115286 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505734
VERSION     XP_077505734.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649608.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..355
                     /product="uncharacterized protein LOC144115286 isoform X4"
                     /calculated_mol_wt=37626
     Region          10..90
                     /region_name="PDZ_ZASP52-like"
                     /note="PDZ domain of Drosophila melanogaster PDZ and LIM
                     domain protein Zasp52 (also known as Zasp), and related
                     domains; cd23068"
                     /db_xref="CDD:467281"
     Site            order(21..27,39,42,70,74,77..78)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467281"
     Region          280..>337
                     /region_name="DUF4749"
                     /note="Domain of unknown function (DUF4749); pfam15936"
                     /db_xref="CDD:464948"
     Region          280..305
                     /region_name="ZM"
                     /note="ZASP-like motif; smart00735"
                     /db_xref="CDD:128974"
     CDS             1..355
                     /gene="LOC144115286"
                     /coded_by="XM_077649608.1:203..1270"
                     /db_xref="GeneID:144115286"
ORIGIN      
        1 msyqkggvls vglrrdfsat pwgfrlqggf dlnapltiqr vflgspsege lhrgdlvlki
       61 gdrdtsrmth neatelirns gnnlslvihr spgphspast pttpkapgma agghplagat
      121 yrpagppayv spqspgitit tqnvsvpapg pyqdgqyqye lrqgflvpep lvenkidalk
      181 eqakitnqty rttplitpsa kprrdvtigs ylrhvkepyf ttmkmpvdsl nnpdaiakkv
      241 qetvnqvasg snspcfglpa svavsggsgg rstpgadgpk ivhnvynspi hlystqnvad
      301 tlaaqagvkl gqgnlqpqtr lasllkeail taskmvrsvl aprvvlpaii hhact
//
LOCUS       XP_077505735             330 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115286 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077505735
VERSION     XP_077505735.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649609.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..330
                     /product="uncharacterized protein LOC144115286 isoform X5"
                     /calculated_mol_wt=35086
     Region          10..90
                     /region_name="PDZ_ZASP52-like"
                     /note="PDZ domain of Drosophila melanogaster PDZ and LIM
                     domain protein Zasp52 (also known as Zasp), and related
                     domains; cd23068"
                     /db_xref="CDD:467281"
     Site            order(21..27,39,42,70,74,77..78)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467281"
     Region          280..305
                     /region_name="ZM"
                     /note="ZASP-like motif; smart00735"
                     /db_xref="CDD:128974"
     CDS             1..330
                     /gene="LOC144115286"
                     /coded_by="XM_077649609.1:203..1195"
                     /db_xref="GeneID:144115286"
ORIGIN      
        1 msyqkggvls vglrrdfsat pwgfrlqggf dlnapltiqr vflgspsege lhrgdlvlki
       61 gdrdtsrmth neatelirns gnnlslvihr spgphspast pttpkapgma agghplagat
      121 yrpagppayv spqspgitit tqnvsvpapg pyqdgqyqye lrqgflvpep lvenkidalk
      181 eqakitnqty rttplitpsa kprrdvtigs ylrhvkepyf ttmkmpvdsl nnpdaiakkv
      241 qetvnqvasg snspcfglpa svavsggsgg rstpgadgpk ivhnvynspi hlystqnvad
      301 tlaaqagvkl gqgnlqpvfi dlsflgrkhh
//
LOCUS       XP_077505736             649 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115287 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505736
VERSION     XP_077505736.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649610.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..649
                     /product="uncharacterized protein LOC144115287 isoform X1"
                     /calculated_mol_wt=68402
     Region          <6..185
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..649
                     /gene="LOC144115287"
                     /coded_by="XM_077649610.1:692..2641"
                     /db_xref="GeneID:144115287"
ORIGIN      
        1 mswqqpggpf gpnmpmtpgg sadpnrmafa snrtptspqw sapqwppape tpdapvyyst
       61 gglfpmqsgg srspsagqrt aspngrfnas gpkfpipppp pagamspaaq nsfsippppp
      121 agqgraspss ggyspntfqi pppppaggrq gnasptvsgg grspgaffip pppaqslggm
      181 assptcatag yddqsyaypp ppppiasssp vsggygsggp frhepptppp ppppqtsmgs
      241 phtaappppp plppvqsggg gpggpppppp ppppppsggg ptwrqkqeae kaaqrssgsg
      301 ydpaqiaaqg pqdgtqpfcy lppqlvgtln skdkkpftyt pggldlseik spriqrrina
      361 rqeaaaaqge gwgdppsrqm eglnldgspq qqdrymdpnr thslprppaa gqrasdpgdf
      421 vqsrsfrvlq kitdtdspey eppappkpre edemrfsglr rdqipsrsfq vlqkmtgadd
      481 qpyqqqqqyy yppqqqeppp direycnhpe elddttinpr yrggnipsrs fkmlqemtgd
      541 dgsvapsvfd qkrqqqqqqq pqagpkprkt tqmtiqlggp epggycgpqg qsgvghvpek
      601 vfphpsvpas qqeaepdprk ytggsipsrs frmlqamtge ntgdggtdf
//
LOCUS       XP_077505737             648 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115287 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505737
VERSION     XP_077505737.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649611.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..648
                     /product="uncharacterized protein LOC144115287 isoform X2"
                     /calculated_mol_wt=68331
     Region          <6..185
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..648
                     /gene="LOC144115287"
                     /coded_by="XM_077649611.1:692..2638"
                     /db_xref="GeneID:144115287"
ORIGIN      
        1 mswqqpggpf gpnmpmtpgg sadpnrmafa snrtptspqw sapqwppape tpdapvyyst
       61 gglfpmqsgg srspsagqrt aspngrfnas gpkfpipppp pagamspaaq nsfsippppp
      121 agqgraspss ggyspntfqi pppppaggrq gnasptvsgg grspgaffip pppaqslggm
      181 assptcatag yddqsyaypp ppppiasssp vsggygsggp frhepptppp ppppqtsmgs
      241 phtaappppp plppvqsggg gpggpppppp ppppppsggg ptwrqkqeae kaaqrssgsg
      301 ydpaqiaaqg pqdgtqpfcy lppqlvgtln skdkkpftyt pggldlseik spriqrrina
      361 rqeaaaaqge gwgdppsrqm eglnldgspq qqdrymdpnr thslprppaa gqrasdpgdf
      421 vqsrsfrvlq kitdtdspey eppppkpree demrfsglrr dqipsrsfqv lqkmtgaddq
      481 pyqqqqqyyy ppqqqepppd ireycnhpee lddttinpry rggnipsrsf kmlqemtgdd
      541 gsvapsvfdq krqqqqqqqp qagpkprktt qmtiqlggpe pggycgpqgq sgvghvpekv
      601 fphpsvpasq qeaepdprky tggsipsrsf rmlqamtgen tgdggtdf
//
LOCUS       XP_077505738             147 aa            linear   INV 25-NOV-2025
DEFINITION  UPAR/Ly6 domain-containing protein crok-like [Amblyomma
            americanum].
ACCESSION   XP_077505738
VERSION     XP_077505738.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649612.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..147
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..147
                     /product="UPAR/Ly6 domain-containing protein crok-like"
                     /calculated_mol_wt=16410
     Region          27..123
                     /region_name="TFP"
                     /note="three-fingered protein (TFP) fold found in Ly6/uPAR
                     (LU) and snake toxin superfamily; cl45932"
                     /db_xref="CDD:480272"
     CDS             1..147
                     /gene="LOC144115288"
                     /coded_by="XM_077649612.1:582..1025"
                     /db_xref="GeneID:144115288"
ORIGIN      
        1 mavarstqvs lvgvvlftlm fvhkgwaiyc wrcnsaydpn cadpfnnits dlvncdmrvk
       61 dhlppgsraq vcrkivqkvy sdfrtvrdcg wlkdekegts cmrragtfsv lmkycscdkd
      121 gcnsarrssa ssllmvappl lllrlfk
//
LOCUS       XP_077505739             366 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-1-like [Amblyomma americanum].
ACCESSION   XP_077505739
VERSION     XP_077505739.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649613.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..366
                     /product="protein argonaute-1-like"
                     /calculated_mol_wt=41530
     Region          <2..>366
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     CDS             1..366
                     /gene="LOC144115289"
                     /coded_by="XM_077649613.1:761..1861"
                     /db_xref="GeneID:144115289"
ORIGIN      
        1 mlnadmsata fyeslplvdf mcrffsdsrc vltpadfqsl renqyvrlnt elkglrvkvt
       61 hlpyprkykv vkitreaakd ihfesdgsqi fiadylqsry rrlsyphfpc vqsgspthpv
      121 yiplevcela egqhcqkkls esqtaemikr takppakrfq eirqsehdlv sstdkylref
      181 gikintepte vkgrvldpps kifgdntmck prdgtwelrg qglykamsmt qwivlnvsrf
      241 aqkdcldnfi kmlipigqel gmriaqplav itfdtnrkam rtvlteqrkq hpqlemvvav
      301 itkatnyaei kqvaetelsl ctqcildnnv vqkcnaaliq nlcqkintnm gsinkspdag
      361 eaeaip
//
LOCUS       XP_077505740             189 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115290 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505740
VERSION     XP_077505740.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649614.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..189
                     /product="uncharacterized protein LOC144115290 isoform X1"
                     /calculated_mol_wt=21554
     CDS             1..189
                     /gene="LOC144115290"
                     /coded_by="XM_077649614.1:1424..1993"
                     /db_xref="GeneID:144115290"
ORIGIN      
        1 mdvanlerfa diveasqsir rlnmmmvprq ksiiflrrfs agitanytlt rvvlltriep
       61 sddwfcvmdt arrnsdlvnr aivfvtasrf dkycatalkp vaehrailqe faeiatvsea
      121 eaagmirsal yslqdmhnfm ravgvvkkrv vcypredvam qldelhfdcw lhvrqhlqle
      181 dvkypgpss
//
LOCUS       XP_077505741             122 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-1-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505741
VERSION     XP_077505741.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649615.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..122
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..122
                     /product="protein argonaute-1-like isoform X2"
                     /calculated_mol_wt=13221
     Region          <2..42
                     /region_name="Piwi-like"
                     /note="PIWI domain. Domain found in proteins involved in
                     RNA silencing. RNA silencing refers to a group of related
                     gene-silencing mechanisms mediated by short RNA molecules,
                     including siRNAs, miRNAs, and heterochromatin-related
                     guide RNAs. The central component...; cl00628"
                     /db_xref="CDD:412485"
     CDS             1..122
                     /gene="LOC144115290"
                     /coded_by="XM_077649615.1:237..605"
                     /db_xref="GeneID:144115290"
ORIGIN      
        1 mpandrdgvg kfrnvppgtt vdsvvthpld fdfflcshfg iqwsvdlamp stnnsngyrr
       61 fqrsklnlfc lnitpmqgac qkdvsqsasp rgasfhplgq pgggkgallr qdhrlssplh
      121 el
//
LOCUS       XP_077505742             101 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115290 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505742
VERSION     XP_077505742.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649616.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..101
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..101
                     /product="uncharacterized protein LOC144115290 isoform X3"
                     /calculated_mol_wt=10840
     CDS             1..101
                     /gene="LOC144115290"
                     /coded_by="XM_077649616.1:57..362"
                     /db_xref="GeneID:144115290"
ORIGIN      
        1 mkgitrtsss spsdkrrgsd twsvdlamps tnnsngyrrf qrsklnlfcl nitpmqgacq
       61 kdvsqsaspr gasfhplgqp gggkgallrq dhrlssplhe l
//
LOCUS       XP_077505743             155 aa            linear   INV 25-NOV-2025
DEFINITION  deoxycytidylate deaminase-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505743
VERSION     XP_077505743.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649617.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..155
                     /product="deoxycytidylate deaminase-like isoform X1"
                     /calculated_mol_wt=17101
     Region          1..120
                     /region_name="deoxycytidylate_deaminase"
                     /note="Deoxycytidylate deaminase domain. Deoxycytidylate
                     deaminase catalyzes the deamination of dCMP to dUMP,
                     providing the nucleotide substrate for thymidylate
                     synthase. The enzyme binds Zn++, which is required for
                     catalytic activity. The activity of the...; cd01286"
                     /db_xref="CDD:238613"
     Site            order(36,65,67,90..91,94)
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:238613"
     Site            order(65,67,91,94)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:238613"
     CDS             1..155
                     /gene="LOC144115292"
                     /coded_by="XM_077649617.1:240..707"
                     /db_xref="GeneID:144115292"
ORIGIN      
        1 mssaylaamr skdpntqvgc vivneenriv gtgytgmpng lgneevpwgk ssdspvepky
       61 lyvchaemna ifnhhcfklh cctlytthfp cnecakmvvq sgirqvvsld nkhpdepiya
      121 asgliltkag vtfrrhipet kkividfeql aafki
//
LOCUS       XP_077505744             155 aa            linear   INV 25-NOV-2025
DEFINITION  deoxycytidylate deaminase-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505744
VERSION     XP_077505744.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649618.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..155
                     /product="deoxycytidylate deaminase-like isoform X1"
                     /calculated_mol_wt=17101
     Region          1..120
                     /region_name="deoxycytidylate_deaminase"
                     /note="Deoxycytidylate deaminase domain. Deoxycytidylate
                     deaminase catalyzes the deamination of dCMP to dUMP,
                     providing the nucleotide substrate for thymidylate
                     synthase. The enzyme binds Zn++, which is required for
                     catalytic activity. The activity of the...; cd01286"
                     /db_xref="CDD:238613"
     Site            order(36,65,67,90..91,94)
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:238613"
     Site            order(65,67,91,94)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:238613"
     CDS             1..155
                     /gene="LOC144115292"
                     /coded_by="XM_077649618.1:246..713"
                     /db_xref="GeneID:144115292"
ORIGIN      
        1 mssaylaamr skdpntqvgc vivneenriv gtgytgmpng lgneevpwgk ssdspvepky
       61 lyvchaemna ifnhhcfklh cctlytthfp cnecakmvvq sgirqvvsld nkhpdepiya
      121 asgliltkag vtfrrhipet kkividfeql aafki
//
LOCUS       XP_077505745             154 aa            linear   INV 25-NOV-2025
DEFINITION  deoxycytidylate deaminase-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505745
VERSION     XP_077505745.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649619.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..154
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..154
                     /product="deoxycytidylate deaminase-like isoform X2"
                     /calculated_mol_wt=17098
     Region          14..119
                     /region_name="cytidine_deaminase-like"
                     /note="Cytidine and deoxycytidylate deaminase zinc-binding
                     region. The family contains cytidine deaminases,
                     nucleoside deaminases, deoxycytidylate deaminases and
                     riboflavin deaminases. Also included are the apoBec family
                     of mRNA editing enzymes. All members...; cl00269"
                     /db_xref="CDD:444801"
     Site            order(64..66,89..90,93)
                     /site_type="active"
                     /db_xref="CDD:238406"
     Site            order(64,66,90,93)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:238406"
     CDS             1..154
                     /gene="LOC144115292"
                     /coded_by="XM_077649619.1:95..559"
                     /db_xref="GeneID:144115292"
ORIGIN      
        1 mastveeekk essrrtvgcv ivneenrivg tgytgmpngl gneevpwgks sdspvepkyl
       61 yvchaemnai fnhhcfklhc ctlytthfpc necakmvvqs girqvvsldn khpdepiyaa
      121 sgliltkagv tfrrhipetk kividfeqla afki
//
LOCUS       XP_077505746             245 aa            linear   INV 25-NOV-2025
DEFINITION  YEATS domain-containing protein 4-like [Amblyomma americanum].
ACCESSION   XP_077505746
VERSION     XP_077505746.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649620.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..245
                     /product="YEATS domain-containing protein 4-like"
                     /calculated_mol_wt=27857
     Region          23..159
                     /region_name="YEATS_GAS41_like"
                     /note="YEATS domain found in YEATS domain-containing
                     protein 4 and similar proteins; cd16909"
                     /db_xref="CDD:341128"
     CDS             1..245
                     /gene="LOC144115293"
                     /coded_by="XM_077649620.1:99..836"
                     /db_xref="GeneID:144115293"
ORIGIN      
        1 mavvmpgiip eivqepggrv kgltivkpiv ygnvaryfgk kreedghthq wtvylkpykn
       61 edmssyvkkv hfklhesypn qnrvvtkppy evtetgwgef eivikiyfmd tterpvtvyh
      121 ilklfqsetn imlgkkqlvs esydelifse ptvmmhqhlt ntrpltvgpy khefdfeekk
      181 ektlhailsa kskirlevad lkerlkvare tidkykaeia klesqddadt gagaaapsaa
      241 fltgl
//
LOCUS       XP_077505747             245 aa            linear   INV 25-NOV-2025
DEFINITION  YEATS domain-containing protein 4-like [Amblyomma americanum].
ACCESSION   XP_077505747
VERSION     XP_077505747.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649621.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..245
                     /product="YEATS domain-containing protein 4-like"
                     /calculated_mol_wt=27857
     Region          23..159
                     /region_name="YEATS_GAS41_like"
                     /note="YEATS domain found in YEATS domain-containing
                     protein 4 and similar proteins; cd16909"
                     /db_xref="CDD:341128"
     CDS             1..245
                     /gene="LOC144115293"
                     /coded_by="XM_077649621.1:148..885"
                     /db_xref="GeneID:144115293"
ORIGIN      
        1 mavvmpgiip eivqepggrv kgltivkpiv ygnvaryfgk kreedghthq wtvylkpykn
       61 edmssyvkkv hfklhesypn qnrvvtkppy evtetgwgef eivikiyfmd tterpvtvyh
      121 ilklfqsetn imlgkkqlvs esydelifse ptvmmhqhlt ntrpltvgpy khefdfeekk
      181 ektlhailsa kskirlevad lkerlkvare tidkykaeia klesqddadt gagaaapsaa
      241 fltgl
//
LOCUS       XP_077505748             101 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115294 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505748
VERSION     XP_077505748.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649622.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..101
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..101
                     /product="uncharacterized protein LOC144115294 isoform X1"
                     /calculated_mol_wt=11140
     CDS             1..101
                     /gene="LOC144115294"
                     /coded_by="XM_077649622.1:375..680"
                     /db_xref="GeneID:144115294"
ORIGIN      
        1 mptlyylaaa svfslrradr adqqcqsvsa ssmvsagflt ptsqlpilcc krtqlrgvrr
       61 qtgmvclqhs krilmtaltn qvwtpqqllp lnhhhlgssl f
//
LOCUS       XP_077505749              84 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115294 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505749
VERSION     XP_077505749.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649623.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..84
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..84
                     /product="uncharacterized protein LOC144115294 isoform X2"
                     /calculated_mol_wt=9041
     CDS             1..84
                     /gene="LOC144115294"
                     /coded_by="XM_077649623.1:569..823"
                     /db_xref="GeneID:144115294"
ORIGIN      
        1 mlqedtasrc sspnwdglsa tfeenphdst yqpsldtsaa sssepspprk fivlrtaccn
       61 spisagraia qhnlhrrssa lllw
//
LOCUS       XP_077505750              84 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115294 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505750
VERSION     XP_077505750.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649624.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..84
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..84
                     /product="uncharacterized protein LOC144115294 isoform X2"
                     /calculated_mol_wt=9041
     CDS             1..84
                     /gene="LOC144115294"
                     /coded_by="XM_077649624.1:452..706"
                     /db_xref="GeneID:144115294"
ORIGIN      
        1 mlqedtasrc sspnwdglsa tfeenphdst yqpsldtsaa sssepspprk fivlrtaccn
       61 spisagraia qhnlhrrssa lllw
//
LOCUS       XP_077505751              84 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115294 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505751
VERSION     XP_077505751.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649625.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..84
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..84
                     /product="uncharacterized protein LOC144115294 isoform X2"
                     /calculated_mol_wt=9041
     CDS             1..84
                     /gene="LOC144115294"
                     /coded_by="XM_077649625.1:402..656"
                     /db_xref="GeneID:144115294"
ORIGIN      
        1 mlqedtasrc sspnwdglsa tfeenphdst yqpsldtsaa sssepspprk fivlrtaccn
       61 spisagraia qhnlhrrssa lllw
//
LOCUS       XP_077505752             216 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115296 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505752
VERSION     XP_077505752.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649626.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..216
                     /product="uncharacterized protein LOC144115296 isoform X1"
                     /calculated_mol_wt=24054
     CDS             1..216
                     /gene="LOC144115296"
                     /coded_by="XM_077649626.1:299..949"
                     /db_xref="GeneID:144115296"
ORIGIN      
        1 mcitmmrwpf pgsllqamvv lilaqaahar csvlpptlrl lplhishpgn ipgavdgpfs
       61 vlmcitmmrw pfpgsllqam vvlilaqaah arcsvlpptl rllplhishp ewlflrahsg
      121 assahgalew rrwsqtadrt teflwrcalp qlraitkkth qesfspvhty gevslpeqvq
      181 gvlrrgpkfa veprrsapel lemetyrepl mglshc
//
LOCUS       XP_077505753             203 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115296 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505753
VERSION     XP_077505753.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649627.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..203
                     /product="uncharacterized protein LOC144115296 isoform X2"
                     /calculated_mol_wt=22536
     CDS             1..203
                     /gene="LOC144115296"
                     /coded_by="XM_077649627.1:298..909"
                     /db_xref="GeneID:144115296"
ORIGIN      
        1 mcitmmrwpf pgsllqamvv lilaqaahar csvlpptlrl lplhishpgn ipgavdgpfs
       61 vlmcitmmrw pfpgsllqam vvlilaqaah arcsvlpptl rllplhishp ewlflrahsg
      121 assahgalew rrwsqtadrt teflwrcalp qlraitkkth qesfspvhty gevslpeqvq
      181 gvlrrgpkfa veprrsapel lem
//
LOCUS       XP_077505754             177 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115296 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505754
VERSION     XP_077505754.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649628.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..177
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..177
                     /product="uncharacterized protein LOC144115296 isoform X3"
                     /calculated_mol_wt=18978
     CDS             1..177
                     /gene="LOC144115296"
                     /coded_by="XM_077649628.1:295..828"
                     /db_xref="GeneID:144115296"
ORIGIN      
        1 mcitmmrwpf pgsllqamvv lilaqaahar csvlpptlrl lplhishpgn ipgavdgpfs
       61 vlmcitmmrw pfpgsllqam vvlilaqaah arcsvlpptl rllplhishp gnipgavdgp
      121 fsllmcitvm rwpfpgsllq amvvlilaqa aharcsvlpp tlrllplhis hpenvss
//
LOCUS       XP_077505755             177 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115296 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505755
VERSION     XP_077505755.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649629.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..177
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..177
                     /product="uncharacterized protein LOC144115296 isoform X4"
                     /calculated_mol_wt=18936
     CDS             1..177
                     /gene="LOC144115296"
                     /coded_by="XM_077649629.1:297..830"
                     /db_xref="GeneID:144115296"
ORIGIN      
        1 mcitmmrwpf pgsllqamvv lilaqaahar csvlpptlrl lplhishpgn ipgavdgpfs
       61 vlmcitmmrw pfpgsllqam vvlilaqaah arcsvlpptl rllplhishp gnipgavdgp
      121 fsllmcitvm rwpfpgsllq amvvlilaqa aharcsvlpp tlrllplhis hpengss
//
LOCUS       XP_077505756             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115297 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505756
VERSION     XP_077505756.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649630.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144115297 isoform X1"
                     /calculated_mol_wt=15193
     CDS             1..133
                     /gene="LOC144115297"
                     /coded_by="XM_077649630.1:529..930"
                     /db_xref="GeneID:144115297"
ORIGIN      
        1 mkrigyhsvk eqdkaerhkc svqlqsafht plfanqlady ttaaathhil swrgsrmsnl
       61 tkrngsfpll lpgsfqrral phdamnddir qwmkneatrs srrriirsrk ystaltastf
      121 pvqlsrpwtr gvs
//
LOCUS       XP_077505757             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115297 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505757
VERSION     XP_077505757.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649631.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144115297 isoform X1"
                     /calculated_mol_wt=15193
     CDS             1..133
                     /gene="LOC144115297"
                     /coded_by="XM_077649631.1:1892..2293"
                     /db_xref="GeneID:144115297"
ORIGIN      
        1 mkrigyhsvk eqdkaerhkc svqlqsafht plfanqlady ttaaathhil swrgsrmsnl
       61 tkrngsfpll lpgsfqrral phdamnddir qwmkneatrs srrriirsrk ystaltastf
      121 pvqlsrpwtr gvs
//
LOCUS       XP_077505758             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115297 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505758
VERSION     XP_077505758.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649632.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144115297 isoform X1"
                     /calculated_mol_wt=15193
     CDS             1..133
                     /gene="LOC144115297"
                     /coded_by="XM_077649632.1:346..747"
                     /db_xref="GeneID:144115297"
ORIGIN      
        1 mkrigyhsvk eqdkaerhkc svqlqsafht plfanqlady ttaaathhil swrgsrmsnl
       61 tkrngsfpll lpgsfqrral phdamnddir qwmkneatrs srrriirsrk ystaltastf
      121 pvqlsrpwtr gvs
//
LOCUS       XP_077505759             126 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115297 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505759
VERSION     XP_077505759.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649633.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..126
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..126
                     /product="uncharacterized protein LOC144115297 isoform X2"
                     /calculated_mol_wt=13673
     CDS             1..126
                     /gene="LOC144115297"
                     /coded_by="XM_077649633.1:1480..1860"
                     /db_xref="GeneID:144115297"
ORIGIN      
        1 mkrnavlisg cadasgvler hceaddshda cvclqnkrer vsstsdpgta gaavqwefst
       61 sanakskhcl sdgplkfhqc gyeelqeadh qeqeifdcfd rfnvpcaavt pldtrsllkt
      121 nnsiev
//
LOCUS       XP_077505760             126 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115297 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505760
VERSION     XP_077505760.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649634.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..126
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..126
                     /product="uncharacterized protein LOC144115297 isoform X2"
                     /calculated_mol_wt=13673
     CDS             1..126
                     /gene="LOC144115297"
                     /coded_by="XM_077649634.1:880..1260"
                     /db_xref="GeneID:144115297"
ORIGIN      
        1 mkrnavlisg cadasgvler hceaddshda cvclqnkrer vsstsdpgta gaavqwefst
       61 sanakskhcl sdgplkfhqc gyeelqeadh qeqeifdcfd rfnvpcaavt pldtrsllkt
      121 nnsiev
//
LOCUS       XP_077505761              65 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115297 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505761
VERSION     XP_077505761.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649635.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..65
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..65
                     /product="uncharacterized protein LOC144115297 isoform X3"
                     /calculated_mol_wt=7524
     CDS             1..65
                     /gene="LOC144115297"
                     /coded_by="XM_077649635.1:752..949"
                     /db_xref="GeneID:144115297"
ORIGIN      
        1 mkrigyhsvk eqdkaerhkc yeelqeadhq eqeifdcfdr fnvpcaavtp ldtrsllktn
       61 nsiev
//
LOCUS       XP_077505762             153 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115300 [Amblyomma americanum].
ACCESSION   XP_077505762
VERSION     XP_077505762.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649636.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..153
                     /product="uncharacterized protein LOC144115300"
                     /calculated_mol_wt=16156
     CDS             1..153
                     /gene="LOC144115300"
                     /coded_by="XM_077649636.1:1..462"
                     /db_xref="GeneID:144115300"
ORIGIN      
        1 miasvsepai lrpslspady vdssgspgyv ftegpcdnrs lmdaswayak gyarhrkkfr
       61 lsdfmpvlad sslslstgps frksqmaspc eediraaaas aaaaaagsns ahrtrtlgeq
      121 pqdttavavf tfalvlknng artflvvsvy deq
//
LOCUS       XP_077505763            2431 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: spectrin alpha chain-like [Amblyomma
            americanum].
ACCESSION   XP_077505763
VERSION     XP_077505763.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649637.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2431
                     /product="LOW QUALITY PROTEIN: spectrin alpha chain-like"
                     /calculated_mol_wt=279285
     Region          47..257
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            150..155
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          153..363
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            256..261
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          260..469
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            362..367
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          470..679
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            574..579
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          576..786
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            679..684
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          683..892
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            785..790
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          892..>956
                     /region_name="Spectrin"
                     /note="Spectrin repeat; pfam00435"
                     /db_xref="CDD:395348"
     Region          973..1025
                     /region_name="SH3_Alpha_Spectrin"
                     /note="Src homology 3 domain of Alpha Spectrin; cd11808"
                     /db_xref="CDD:212742"
     Site            order(978,980,983,1005..1006,1019,1021..1022)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212742"
     Region          1073..1285
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            1178..1183
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1286..1508
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            1404..1409
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1406..1616
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            1508..1513
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1617..1828
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            1721..1726
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1830..2041
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            1933..1938
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1936..2151
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            2040..2045
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          2054..2270
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            2154..2159
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          2285..2353
                     /region_name="EFh"
                     /note="EF-hand, calcium binding motif; A diverse
                     superfamily of calcium sensors and calcium signal
                     modulators; most examples in this alignment model have 2
                     active canonical EF hands. Ca2+ binding induces a
                     conformational change in the EF-hand motif, leading to...;
                     cd00051"
                     /db_xref="CDD:238008"
     Site            order(2294,2296,2298,2305,2337,2339,2341,2348)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238008"
     Region          2360..2430
                     /region_name="EFhand_Ca_insen"
                     /note="Ca2+ insensitive EF hand; pfam08726"
                     /db_xref="CDD:430177"
     CDS             1..2431
                     /gene="LOC144115302"
                     /coded_by="XM_077649637.1:156..7451"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 1 base in 1 codon"
                     /db_xref="GeneID:144115302"
ORIGIN      
        1 mdvqprdiki letvddiqer reqvlgrysq fksearhkrd rleesrrfqy fkrdadeles
       61 wiheklqaas desykdatnl qakiqkhqaf eaevaahgna ivvldntgme migyghfese
      121 kikqcldelh rlwelllxki adkgqklqqa lvlvqflrhc devmfwindk etfvmadefg
      181 qdlehvevlq rkfdefqkdm asqefrvtev neqaeklind dhperetirk rreelneawn
      241 klkaltllrq erlfgaheiq rfnrdadeti twitekdvvl ssddygrdlv svqtlqrkhe
      301 gierdlaale dkvmtlgqea drlcsihsdh gsqirgkhae imatwemlkr kaqerrrrld
      361 esyllhrfla dfrdlvswih dmnaiisade lakdvagaea llerhqehkg eidaredsfr
      421 staeagqill dqkhyavdev keklgilene ksvllalwee rrilyeqcmd lqlfyrdteq
      481 adtwmakqea flanqdlgds ldsveallkk hedfekslna qeekikalde fatkliegqh
      541 yaaddvaqrr aallerrnal lershtrhtm leesyrlqqf erdcdetkgw ineklkiamd
      601 esyldptnln gkvqkhqnfi qelnanrsrv editstgqel vdanhyasdr isqrmeeiit
      661 lwqslskaad tkgtklseas aqqqfnrgve dielwlseve gqlmsedcgk dltsvqnllk
      721 khalletdva shadrvdgvm iqaqqfadkg hfdapaimak kaalverynt lqapmatrrq
      781 klqdslrvqq lfrdvedeea wirekepiaa stnrgrdlig vqnlikkhqa vlaeinnhen
      841 rvrsvceage nmvadghfah deinkriqnl serwqqlkdk alqrkrdled slqahqyfad
      901 aneaeswmke kepivgsqdy gkdedsaeal lkkhealmad leafgnsvea lkeqaqlcrq
      961 qeapivdqag kefvmalydy teksprevsm kkndilalln snnkdwwkve vndrqgfvpa
     1021 ayvkkveagl sasqqhlaeg nsigarqaqi egqygnlmal grsrcdklae slrayqlvre
     1081 aaelaqwikd keqvaqvqev gedleqvevl qkkfddfmad lkgnevrlge mnnianklmt
     1141 lgqteaavki qtqiedlnqk wqhlqevtqe raqqlgsahe vqrfhrdvde tkdwiqekdd
     1201 glladdcgnd lrsvqtlqrk heglerdlta lgdrirqlde tagrlvnthp estesmitkk
     1261 qeiiqewtrl takakarkek lldsydlqrf ladyrdltsw insmmalvss delandvtga
     1321 eallerhlty rseidarygi pqehrteida ragtfqafem fgqqllqngh yasveiqqkl
     1381 dmmtearkel ekawiarrvk vdqcldlqlf yrdceqaenw masreaflgs ddmggdnvea
     1441 likkhedfdk aigaqeekia alaaladklv ssdhyagndi kdkkeqvlnr wkhlkealie
     1501 krsrlgesqt lqqfsrdade ienwiaeklq mamdesykdp aniqskhkkh qafeaelaan
     1561 adriqavlam gqnlidkrkc agsedavqar lgsiadqweh lttksseksm klkeankqrt
     1621 fnaavkdidf wlgevesllk sedsgkdlas vqnltkkhql veadilahed rvkdlnalad
     1681 slvdsgqfhs gpiqekrssi neryerlrtl aayrrsqlhe antlhqffrd iadeeswike
     1741 kkllvgsddy grdltgvqnl rkkhkrlese lgahepaiqa vqeaghklma esnlgvpeie
     1801 qrlqtlelsw relkqmagdr ghkldeslvy qqflakveee eawitekqql lsvedcgdtm
     1861 aavqgllkkh eafeadfavh rercgdtvaa gqglvqegnh hkesiqqrld qlvqrldale
     1921 asaarrrakl qdnsaylqfm wkadvveswi adkeahvrse dfgrdlssvq tlltkqetfd
     1981 aglgafeqeg iqsitqlkdq lvaanhdqta aimrrhddvl trwnnllaas narklrllhm
     2041 leqfkqiedl fltfakkasa fnswfenaee dltdpvrcns veeiralrea haqfqnslgs
     2101 aqadfealaa ldrqiksfnv gpnpytwftm ealedtwrnl qkiikerdle lgkeaqrqed
     2161 ndhlrrefak hanalhqwlt dtrmwlldgs smmegsgsle aqleatkrka aevrgkrgdl
     2221 kriedlgall eeqlildnry tehgtvglaq qwdqldqlgm rmqhnleqqi qarnqsgvte
     2281 dalkefsmmf khfdkdksgr lnhvefkscl ralgydlpmv eegqpdpefe aildqvdpnr
     2341 dgqvslqeym afmisreten vrsseeiena fraitsgerp yvtadelyan ltkdmadycv
     2401 srmkpyvdpk sgrsisgayd yieftrtlfq n
//
LOCUS       XP_077505764             594 aa            linear   INV 25-NOV-2025
DEFINITION  kelch-like protein diablo [Amblyomma americanum].
ACCESSION   XP_077505764
VERSION     XP_077505764.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649638.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..594
                     /product="kelch-like protein diablo"
                     /calculated_mol_wt=66446
     Region          30..157
                     /region_name="BTB_POZ_KLHL20_KLEIP"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in Kelch-like
                     protein 20 (KLHL20); cd18249"
                     /db_xref="CDD:349558"
     Region          50..568
                     /region_name="PHA03098"
                     /note="kelch-like protein; Provisional"
                     /db_xref="CDD:222983"
     Region          340..384
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          388..432
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          435..480
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          482..526
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          529..574
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     CDS             1..594
                     /gene="dbo"
                     /coded_by="XM_077649638.1:404..2188"
                     /db_xref="GeneID:144115303"
ORIGIN      
        1 mndircfrst mgeilhtdld sstsparley tsekhsrhtl eainllrlhr elcdvvllvg
       61 nrkifahriv lsacspyfha mftgelaesr qtevtirdid ehamellmdf aytshivvee
      121 gnvqmllpaa cllqmaeiqd vcceflkrql dptnclgira fadthscrel lriadkftqh
      181 nfqevmesee flllpvnqlv diissdelnv rseeqvfsav mswvkynvte rrqnlgqvlq
      241 hvrlpllspk flvgtvgsdl liksdevcrd lvdeaknyll lpqerplmqg prtrprkpvr
      301 rgevlfavgg wcsgdaiasv erydpqanew rmvapmskrr cgvgvavlnd llyavgghdg
      361 qsylnsiery dpqtnqwssd vaptsscrts vgvavldgyl yavggqdgvs clnfverydp
      421 qtnrwakvap mstkrlgvav avlgsylyam ggsdgtspln tverydprtn rwgsvasmgt
      481 rrkhlgsavy snmiyavggr ddttelssae rynpqlnqwq pivamtsrrs gvglavvngl
      541 lyavggfdgt tylktievyd peqnqwklcg smnyrrlggg vgvvrlpqhe ahyw
//
LOCUS       XP_077505766             584 aa            linear   INV 25-NOV-2025
DEFINITION  inositol-3-phosphate synthase [Amblyomma americanum].
ACCESSION   XP_077505766
VERSION     XP_077505766.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649640.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..584
                     /product="inositol-3-phosphate synthase"
                     /calculated_mol_wt=65499
     Region          240..536
                     /region_name="EEP"
                     /note="Exonuclease-Endonuclease-Phosphatase (EEP) domain
                     superfamily; cl00490"
                     /db_xref="CDD:469791"
     Site            order(252,284,368,419,421,500,527..528)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197329"
     Site            order(284,527)
                     /site_type="other"
                     /note="putative metal binding site [ion binding]"
                     /db_xref="CDD:197329"
     Site            order(368,421,528)
                     /site_type="other"
                     /note="putative phosphate binding site [ion binding]"
                     /db_xref="CDD:197329"
     CDS             1..584
                     /gene="INPP5E"
                     /coded_by="XM_077649640.1:147..1901"
                     /db_xref="GeneID:144115304"
ORIGIN      
        1 mdrsqpaske dgppkrpsrl tcrkdrikgg tkksqslpvi ktpledlrlv hnnsvtaplm
       61 hgsatsssdd eafelapssf alkdnlsegp sqapnlrprv elvrlsslvk cpsstslnrp
      121 lsfateqllr qtscvstgsl kslsdgtngs ygnkphqlpd aekpepsgst rcvtevkklh
      181 hswtvsgmte fqdsvdfppd qtsilpkist kdarlrsylv gnvsgkgsll geeelnryfp
      241 drkvnifvat wnmnglaspa slddlllpkt idyvpdiyai gvqeamsdkk ewetrlqftl
      301 gpshvlfysv slgvlnlsif lrrdliwfcs aaeeatyatr pvssvktkga vaiafqffgs
      361 smlfinshla ahekkvkeri qdyekichqi dlpkqaqlkl qyqskdvtar fdhvfwcgdl
      421 nfrlmnersi vvnllkdhse dkettydnll kydqlrsaml dgqaffgfqe gkikfgptyk
      481 fevgtshfdg vklrvpsytd rilyrskrkg hiqciyydsi tmmqtsdhrp vyglyetvir
      541 pgrdntrlsa glfnrdvyle avrrraaale irqslkasqv cvvm
//
LOCUS       XP_077505767             383 aa            linear   INV 25-NOV-2025
DEFINITION  tachykinin-like peptides receptor 99D [Amblyomma americanum].
ACCESSION   XP_077505767
VERSION     XP_077505767.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649641.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..383
                     /product="tachykinin-like peptides receptor 99D"
                     /calculated_mol_wt=43319
     Region          62..351
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          64..88
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320515"
     Region          97..119
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320515"
     Region          135..157
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320515"
     Region          179..195
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320515"
     Region          228..251
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320515"
     Region          282..304
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320515"
     Region          319..344
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320515"
     CDS             1..383
                     /gene="LOC144115305"
                     /coded_by="XM_077649641.1:740..1891"
                     /db_xref="GeneID:144115305"
ORIGIN      
        1 masslllsrp mdlepyssrq gdtlalnvsw aapprnvadv agasasptve erlykvpvev
       61 ivilsicyga islvavvgnt fvlwivatsr rmrtetnyli anlaisdiii glfsipfhfq
      121 aallqrwvlp rfmcafcpfv qvlsvnvsif tltaiavdry raitiplkar lnsrltarvl
      181 igviwvasav aaapyafalr vslvqdertg ertrpfchnv alppgawriy nnalvcsqyf
      241 apllvicyvy erirrllkds tipgnpetvr daivvrnkkk vtkmmfivva ifalcwlpyq
      301 tynilteiyp ainsyryinv iwfimhwlam snscynpfiy aiysekfnte fkarirccar
      361 rhheeiiygh ssimtqlsvr lqs
//
LOCUS       XP_077505768             253 aa            linear   INV 25-NOV-2025
DEFINITION  kynurenine aminotransferase-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505768
VERSION     XP_077505768.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649642.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..253
                     /product="kynurenine aminotransferase-like isoform X1"
                     /calculated_mol_wt=28500
     Region          <1..236
                     /region_name="PRK08912"
                     /note="aminotransferase"
                     /db_xref="CDD:181580"
     CDS             1..253
                     /gene="LOC144115307"
                     /coded_by="XM_077649642.1:141..902"
                     /db_xref="GeneID:144115307"
ORIGIN      
        1 millntpqnp lgkvysreel emvaqlcrah dvicvmdevy ewiiyegvkh vrmntlpdmw
       61 ertitigsag kafsitgwrv gwaygpqhll kalqsvhhng vyacstilqe avavglekem
      121 ertadqsgyw kglssllqkk rdyicgylss lgvtatvpqg syfivcdfsr iasklkleat
      181 epkhlqfsdw lhrtkklksf pgssyfsnrq spfaqylvrf cfakddstlr qaaeilmele
      241 telknatgrg sst
//
LOCUS       XP_077505769             224 aa            linear   INV 25-NOV-2025
DEFINITION  kynurenine aminotransferase-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505769
VERSION     XP_077505769.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649643.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..224
                     /product="kynurenine aminotransferase-like isoform X2"
                     /calculated_mol_wt=25113
     Region          <1..172
                     /region_name="PRK08912"
                     /note="aminotransferase"
                     /db_xref="CDD:181580"
     CDS             1..224
                     /gene="LOC144115307"
                     /coded_by="XM_077649643.1:151..825"
                     /db_xref="GeneID:144115307"
ORIGIN      
        1 millntpqnp lgkvysreel emvaqlcrah dvicvmdevy ewiiyegvkh vrmntlpdmw
       61 ertitigsag kafsitgwrv gwaygpqhll kalqsvhhng vyacstilqe avavglekem
      121 ertadqsgyw kglssllqkk rdyicgylss lgvtatvpqg syfivcdfsr iasklkleat
      181 epkhlqfsdw lhrtkddstl rqaaeilmel etelknatgr gsst
//
LOCUS       XP_077505770             427 aa            linear   INV 25-NOV-2025
DEFINITION  isovaleryl-CoA dehydrogenase, mitochondrial [Amblyomma americanum].
ACCESSION   XP_077505770
VERSION     XP_077505770.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649644.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (GKSP01013064.1) annotated
            using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 207 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..427
                     /product="isovaleryl-CoA dehydrogenase, mitochondrial"
                     /calculated_mol_wt=46665
     Region          45..424
                     /region_name="IVD"
                     /note="Isovaleryl-CoA dehydrogenase; cd01156"
                     /db_xref="CDD:173845"
     Site            order(128,132,136,175,201,224,278,285,288,291,403..404,
                     407..409)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:173845"
     Site            order(166,168..169,174..175,199,201,410,412)
                     /site_type="other"
                     /note="FAD binding site [chemical binding]"
                     /db_xref="CDD:173845"
     Site            287
                     /site_type="active"
                     /note="catalytic base [active]"
                     /db_xref="CDD:173845"
     CDS             1..427
                     /gene="LOC144115308"
                     /coded_by="XM_077649644.1:21..1304"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 207 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144115308"
ORIGIN      
        1 mlvnmsaasg mwssllrlgk stkglpyrls irkcayypiq dvmyglsaeq kelreaafnf
       61 aqkelaphar didknnhfpg frdfwkklgd mgfmgvtvpa eygglgagyl dhciiqeels
      121 racaaialsy gahsnlcinq iyrngteeqk rkylpklisg efvgslamse agsgsdvasm
      181 sltavrdgdh yilngtkfwi tngsladvvf vyartnplad kpqhgisafi vekntpgfsv
      241 gqqldklgmr gsptaelvfe dckipaknlv gelnkgmyvl msgldferli laagpigimq
      301 accdvafdyv htrkqfgqpi gtfqllqgki admyttlnac rsylystara vdqghvlskd
      361 cagvilfcae kgtqlaldav qclggngyin dyptgrllrd aklyeigagt seirrliigr
      421 tinqeyk
//
LOCUS       XP_077505771             228 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115309 [Amblyomma americanum].
ACCESSION   XP_077505771
VERSION     XP_077505771.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649645.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..228
                     /product="uncharacterized protein LOC144115309"
                     /calculated_mol_wt=25316
     Region          82..164
                     /region_name="MULE"
                     /note="MULE transposase domain; pfam10551"
                     /db_xref="CDD:463145"
     CDS             1..228
                     /gene="LOC144115309"
                     /coded_by="XM_077649645.1:2192..2878"
                     /db_xref="GeneID:144115309"
ORIGIN      
        1 myeawrethy gtvseplmrl qekipeyteq gvdvkvhseg gagswavllv tpimrcaqql
       61 esssevifvd stascdstrs svtimlttts ggaipiaviv hssqttegys agfellkhny
      121 pfcfggceap etfmtdnsaa ekaailehrp earqqlchfh vaqaewrwlt lakhgvspse
      181 rkslmaifqk imyanskdtl eaakqdlysq qhkgfithvd tllereee
//
LOCUS       XP_077505772             202 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115314 [Amblyomma americanum].
ACCESSION   XP_077505772
VERSION     XP_077505772.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649646.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..202
                     /product="uncharacterized protein LOC144115314"
                     /calculated_mol_wt=21814
     CDS             1..202
                     /gene="LOC144115314"
                     /coded_by="XM_077649646.1:4321..4929"
                     /db_xref="GeneID:144115314"
ORIGIN      
        1 mctllphlkh cheighinkv rrallidrrh arlfaceerl plsvgmksnh vitvaghflq
       61 rgkwsrytrn tsslddtctv pklppgllak llpaattvrr plgklvqlvv qaihkslqra
      121 lrvqhvylel qcpapaeyvi afaaasqqhi ssaicalstc klalggdsga avvsvtssll
      181 tappgyikgt dsprtlgicg hw
//
LOCUS       XP_077505773            1440 aa            linear   INV 25-NOV-2025
DEFINITION  prosaposin isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505773
VERSION     XP_077505773.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649647.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1440
                     /product="prosaposin isoform X1"
                     /calculated_mol_wt=160706
     Region          66..142
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          187..259
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          281..355
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          445..519
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          534..609
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          627..702
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          720..795
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          815..889
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          913..986
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          1005..1079
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          1087..1162
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          1176..1251
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          1271..1298
                     /region_name="SapA"
                     /note="Saposin A-type domain; pfam02199"
                     /db_xref="CDD:460487"
     CDS             1..1440
                     /gene="Sap-r"
                     /coded_by="XM_077649647.1:137..4459"
                     /db_xref="GeneID:144115316"
ORIGIN      
        1 mfarvllaas viclsnaipl vkpsdkvcqt lstscqdlwv asscktvkpc iemvweklev
       61 pmdkssvcni ckemvkeard qllsnetqee lrevlegsca lipigfisdl ckkmvddfip
      121 dlidilvsrl dpdqvctiag lcnpdfvtkk lqsllqhrvl masiqsrifl plakvstavs
      181 dtkktatcte cktamkyare lfqklplarv ksavlkvcqe klgstnpecf tvvdvllpsv
      241 fkylqsvdpe efcaylgrck altqsppkls ippitqlkdn ltcdfckqvv ehvrqilasn
      301 tteqefkqal lnfceelgsa aeecqtfide yfdmvysyll eafdpdtfct tiglcqrgvt
      361 seeralsklv ikafqgaleq kghlknlkaa kkargylkps ptvplikvyp avpkkyldsn
      421 ksdiafpmvk tfpaqyaptv ediytcsick afitlleqvv plnatvddvk flldeicelf
      481 paetekncrt fvdknseiil nflthdvapa vicheimlcs plppkktgiy kdspdcdyck
      541 ltveffydel kkketeeeik rvvekfcqlf paaskdkcis tinsyvdmvi slilqeftpe
      601 qicqelkfca stvvsapvvp pkkvsddkcd lcmvvvkfvy dqikdektee rvkqaldqvc
      661 sllpdslqqk cvnmvntyyd mlvnlllqel vpeqvcqelg lcssavvsap vvppkkvsdd
      721 kcdlcmvvvk fvydqikdek teervkqald qvcsllpdsl qqkcvnmvnt yydmlvnlll
      781 qelvpeqvcq elglcssapg kpmetklpts andeecpvce imvkliynel kdqkteqeik
      841 dvldkvcswm ppsleqrcin tvntyfdlfv tlitqglspe qvcqqiglcp tgkesklpph
      901 igvppkkasd eqcdickviv qfvynelkdk kteeevkqll drvcsllpds lqqtcidtvn
      961 tyydqlvdli vkgftpeqvc qelglctsar kstptkptkv addetctyct tvvqyvydal
     1021 tdekteeeik ealdmactlf egdarkecin ivntyfeiiv slilkdftpe qicqtiglcp
     1081 sapggdlect fcqyalhfiq nelvdnntea rveailenlc aklpvdlake cvafvdeygp
     1141 avmvliaqei dpsivcvaik acpkdglkkq sdlklsecas ctsavsyiek vlksakndqe
     1201 vakqvanacd rvpktvrsec kalirafgps vlqgvaqlgn skvackkldl csaneteeyv
     1261 rrqlkplgah pcsrgpahwc hskenaeack ataycmkkgl letsdrhtga fwakqrqlqt
     1321 ttsrvptlqr ktaaddspqc sfckssidyv erclmnrtav qvmyrvseic elfpetiekp
     1381 crlatdknan iiyiglkyhs dpstlcnimt lcphapsaeg respqvpwgk gcdfgigrep
//
LOCUS       XP_077505774            1439 aa            linear   INV 25-NOV-2025
DEFINITION  prosaposin isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505774
VERSION     XP_077505774.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649648.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1439
                     /product="prosaposin isoform X2"
                     /calculated_mol_wt=160634
     Region          66..142
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          187..259
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          281..355
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          445..519
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          534..609
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          627..702
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          719..794
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          814..888
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          912..985
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          1004..1078
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          1086..1161
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          1175..1250
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          1270..1297
                     /region_name="SapA"
                     /note="Saposin A-type domain; pfam02199"
                     /db_xref="CDD:460487"
     CDS             1..1439
                     /gene="Sap-r"
                     /coded_by="XM_077649648.1:137..4456"
                     /db_xref="GeneID:144115316"
ORIGIN      
        1 mfarvllaas viclsnaipl vkpsdkvcqt lstscqdlwv asscktvkpc iemvweklev
       61 pmdkssvcni ckemvkeard qllsnetqee lrevlegsca lipigfisdl ckkmvddfip
      121 dlidilvsrl dpdqvctiag lcnpdfvtkk lqsllqhrvl masiqsrifl plakvstavs
      181 dtkktatcte cktamkyare lfqklplarv ksavlkvcqe klgstnpecf tvvdvllpsv
      241 fkylqsvdpe efcaylgrck altqsppkls ippitqlkdn ltcdfckqvv ehvrqilasn
      301 tteqefkqal lnfceelgsa aeecqtfide yfdmvysyll eafdpdtfct tiglcqrgvt
      361 seeralsklv ikafqgaleq kghlknlkaa kkargylkps ptvplikvyp avpkkyldsn
      421 ksdiafpmvk tfpaqyaptv ediytcsick afitlleqvv plnatvddvk flldeicelf
      481 paetekncrt fvdknseiil nflthdvapa vicheimlcs plppkktgiy kdspdcdyck
      541 ltveffydel kkketeeeik rvvekfcqlf paaskdkcis tinsyvdmvi slilqeftpe
      601 qicqelkfca stvvsapvvp pkkvsddkcd lcmvvvkfvy dqikdektee rvkqaldqvc
      661 sllpdslqqk cvnmvntyyd mlvnlllqel vpeqvcqelg lcssvvsapv vppkkvsddk
      721 cdlcmvvvkf vydqikdekt eervkqaldq vcsllpdslq qkcvnmvnty ydmlvnlllq
      781 elvpeqvcqe lglcssapgk pmetklptsa ndeecpvcei mvkliynelk dqkteqeikd
      841 vldkvcswmp psleqrcint vntyfdlfvt litqglspeq vcqqiglcpt gkesklpphi
      901 gvppkkasde qcdickvivq fvynelkdkk teeevkqlld rvcsllpdsl qqtcidtvnt
      961 yydqlvdliv kgftpeqvcq elglctsark stptkptkva ddetctyctt vvqyvydalt
     1021 dekteeeike aldmactlfe gdarkecini vntyfeiivs lilkdftpeq icqtiglcps
     1081 apggdlectf cqyalhfiqn elvdnntear veailenlca klpvdlakec vafvdeygpa
     1141 vmvliaqeid psivcvaika cpkdglkkqs dlklsecasc tsavsyiekv lksakndqev
     1201 akqvanacdr vpktvrseck alirafgpsv lqgvaqlgns kvackkldlc saneteeyvr
     1261 rqlkplgahp csrgpahwch skenaeacka taycmkkgll etsdrhtgaf wakqrqlqtt
     1321 tsrvptlqrk taaddspqcs fckssidyve rclmnrtavq vmyrvseice lfpetiekpc
     1381 rlatdknani iyiglkyhsd pstlcnimtl cphapsaegr espqvpwgkg cdfgigrep
//
LOCUS       XP_077505775            1301 aa            linear   INV 25-NOV-2025
DEFINITION  prosaposin isoform X3 [Amblyomma americanum].
ACCESSION   XP_077505775
VERSION     XP_077505775.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649649.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1301
                     /product="prosaposin isoform X3"
                     /calculated_mol_wt=145282
     Region          66..142
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          187..259
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          281..355
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          445..519
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          534..609
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          627..702
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          719..794
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          814..888
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          912..985
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          1004..1078
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          1086..1161
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          1175..1250
                     /region_name="SapB"
                     /note="Saposin (B) Domains; smart00741"
                     /db_xref="CDD:214797"
     Region          1270..1300
                     /region_name="SapA"
                     /note="Saposin A-type domain; pfam02199"
                     /db_xref="CDD:460487"
     CDS             1..1301
                     /gene="Sap-r"
                     /coded_by="XM_077649649.1:137..4042"
                     /db_xref="GeneID:144115316"
ORIGIN      
        1 mfarvllaas viclsnaipl vkpsdkvcqt lstscqdlwv asscktvkpc iemvweklev
       61 pmdkssvcni ckemvkeard qllsnetqee lrevlegsca lipigfisdl ckkmvddfip
      121 dlidilvsrl dpdqvctiag lcnpdfvtkk lqsllqhrvl masiqsrifl plakvstavs
      181 dtkktatcte cktamkyare lfqklplarv ksavlkvcqe klgstnpecf tvvdvllpsv
      241 fkylqsvdpe efcaylgrck altqsppkls ippitqlkdn ltcdfckqvv ehvrqilasn
      301 tteqefkqal lnfceelgsa aeecqtfide yfdmvysyll eafdpdtfct tiglcqrgvt
      361 seeralsklv ikafqgaleq kghlknlkaa kkargylkps ptvplikvyp avpkkyldsn
      421 ksdiafpmvk tfpaqyaptv ediytcsick afitlleqvv plnatvddvk flldeicelf
      481 paetekncrt fvdknseiil nflthdvapa vicheimlcs plppkktgiy kdspdcdyck
      541 ltveffydel kkketeeeik rvvekfcqlf paaskdkcis tinsyvdmvi slilqeftpe
      601 qicqelkfca stvvsapvvp pkkvsddkcd lcmvvvkfvy dqikdektee rvkqaldqvc
      661 sllpdslqqk cvnmvntyyd mlvnlllqel vpeqvcqelg lcssvvsapv vppkkvsddk
      721 cdlcmvvvkf vydqikdekt eervkqaldq vcsllpdslq qkcvnmvnty ydmlvnlllq
      781 elvpeqvcqe lglcssapgk pmetklptsa ndeecpvcei mvkliynelk dqkteqeikd
      841 vldkvcswmp psleqrcint vntyfdlfvt litqglspeq vcqqiglcpt gkesklpphi
      901 gvppkkasde qcdickvivq fvynelkdkk teeevkqlld rvcsllpdsl qqtcidtvnt
      961 yydqlvdliv kgftpeqvcq elglctsark stptkptkva ddetctyctt vvqyvydalt
     1021 dekteeeike aldmactlfe gdarkecini vntyfeiivs lilkdftpeq icqtiglcps
     1081 apggdlectf cqyalhfiqn elvdnntear veailenlca klpvdlakec vafvdeygpa
     1141 vmvliaqeid psivcvaika cpkdglkkqs dlklsecasc tsavsyiekv lksakndqev
     1201 akqvanacdr vpktvrseck alirafgpsv lqgvaqlgns kvackkldlc saneteeyvr
     1261 rqlkplgahp csrgpahwch skenaeacka taycmkkvwk q
//
LOCUS       XP_077505776             430 aa            linear   INV 25-NOV-2025
DEFINITION  pancreatic lipase-related protein 2-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505776
VERSION     XP_077505776.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649650.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..430
                     /product="pancreatic lipase-related protein 2-like isoform
                     X1"
                     /calculated_mol_wt=46708
     Region          141..423
                     /region_name="Pancreat_lipase_like"
                     /note="Pancreatic lipase-like enzymes. Lipases are
                     esterases that can hydrolyze long-chain acyl-triglycerides
                     into di- and monoglycerides, glycerol, and free fatty
                     acids at a water/lipid interface. A typical feature of
                     lipases is 'interfacial activation,' the...; cd00707"
                     /db_xref="CDD:238363"
     Site            262..266
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238363"
     Site            order(264,292,360)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238363"
     CDS             1..430
                     /gene="LOC144115318"
                     /coded_by="XM_077649650.1:186..1478"
                     /db_xref="GeneID:144115318"
ORIGIN      
        1 mvlgsqtsvp tllvaflsmg allvnaapqe spfglgalgn afrsigigrl sslstrtlqa
       61 fqpkvqtnpr lstsspigda aryldssakv fdsvipgqfa ekllpkfddk sdqvcyeyvg
      121 cfsnqdkltl ptsfpsdpsa vdtkfelysr snrrspvrld yrpstrlgrl aqfdkpkplk
      181 livhgfleng kvswiqdikd afldaedcnv iiidwsggaq llsyikaagn talvgreasl
      241 lvqrliethr ntlsvdqvhi vgfslggqia gffgrhfkht tgklvprita ldpagplfdn
      301 tsvclsdkda rfvdaihtsg geaivvgelg ierpvghvdf ypnggkmqpg ckpldipcdh
      361 fratayfles lrnkqcrfvs epcpggfral lqkkcqprgq rglmgyfsdt vagrgvqtli
      421 tnaksqycks
//
LOCUS       XP_077505777             408 aa            linear   INV 25-NOV-2025
DEFINITION  pancreatic lipase-related protein 2-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505777
VERSION     XP_077505777.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649651.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..408
                     /product="pancreatic lipase-related protein 2-like isoform
                     X2"
                     /calculated_mol_wt=44420
     Region          119..401
                     /region_name="Pancreat_lipase_like"
                     /note="Pancreatic lipase-like enzymes. Lipases are
                     esterases that can hydrolyze long-chain acyl-triglycerides
                     into di- and monoglycerides, glycerol, and free fatty
                     acids at a water/lipid interface. A typical feature of
                     lipases is 'interfacial activation,' the...; cd00707"
                     /db_xref="CDD:238363"
     Site            240..244
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238363"
     Site            order(242,270,338)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238363"
     CDS             1..408
                     /gene="LOC144115318"
                     /coded_by="XM_077649651.1:186..1412"
                     /db_xref="GeneID:144115318"
ORIGIN      
        1 mvlgsqtsvp tllvaflsmg allvnaapqe spfglgalfq pkvqtnprls tsspigdaar
       61 yldssakvfd svipgqfaek llpkfddksd qvcyeyvgcf snqdkltlpt sfpsdpsavd
      121 tkfelysrsn rrspvrldyr pstrlgrlaq fdkpkplkli vhgflengkv swiqdikdaf
      181 ldaedcnvii idwsggaqll syikaagnta lvgreasllv qrliethrnt lsvdqvhivg
      241 fslggqiagf fgrhfkhttg klvpritald pagplfdnts vclsdkdarf vdaihtsgge
      301 aivvgelgie rpvghvdfyp nggkmqpgck pldipcdhfr atayfleslr nkqcrfvsep
      361 cpggfrallq kkcqprgqrg lmgyfsdtva grgvqtlitn aksqycks
//
LOCUS       XP_077505778             428 aa            linear   INV 25-NOV-2025
DEFINITION  pancreatic lipase-related protein 2-like [Amblyomma americanum].
ACCESSION   XP_077505778
VERSION     XP_077505778.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649652.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..428
                     /product="pancreatic lipase-related protein 2-like"
                     /calculated_mol_wt=47503
     Region          113..395
                     /region_name="Pancreat_lipase_like"
                     /note="Pancreatic lipase-like enzymes. Lipases are
                     esterases that can hydrolyze long-chain acyl-triglycerides
                     into di- and monoglycerides, glycerol, and free fatty
                     acids at a water/lipid interface. A typical feature of
                     lipases is 'interfacial activation,' the...; cd00707"
                     /db_xref="CDD:238363"
     Site            234..238
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238363"
     Site            order(236,264,332)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238363"
     Site            order(323..324,329..330)
                     /site_type="active"
                     /note="active site flap/lid"
                     /db_xref="CDD:238363"
     CDS             1..428
                     /gene="LOC144115319"
                     /coded_by="XM_077649652.1:216..1502"
                     /db_xref="GeneID:144115319"
ORIGIN      
        1 mgfvlhtcia alvlllatvt ygatvrnndd felttpaedg egvegrirlp gkamrfigas
       61 velldsvlpg nladtvkvrd ksndstcypl vgcfrsrdpm tvplsfpdsp ekvntsllly
      121 srqnrnsplr ldwrsrerne rlnlfrerkp lklivhgwqe tgdsewvqea kdsllnledc
      181 nvivvdwrgg sergnyirsa gntalvgrqg slllqhllti yrqtlstedv hvighslggq
      241 vsgflgrhfl nqtgvrlgri taldaaaplf edtdvflssr daqfvdaiht ssggkvikge
      301 fgilkpfghv dfypnggqkq pgcpplelfc dhklskdffl eslrnrrcrf isepcaggld
      361 alladrcvrr gdrgemgyfa dqapgrglqt letnesppyc rerqvprnps rddtprrlnq
      421 gvrpgrrg
//
LOCUS       XP_077505780             375 aa            linear   INV 25-NOV-2025
DEFINITION  putative phospholipase A1 magnifin [Amblyomma americanum].
ACCESSION   XP_077505780
VERSION     XP_077505780.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649654.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..375
                     /product="putative phospholipase A1 magnifin"
                     /calculated_mol_wt=41123
     Region          83..362
                     /region_name="Abhydrolase"
                     /note="alpha/beta hydrolases; cl21494"
                     /db_xref="CDD:473884"
     Site            198..202
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238382"
     Site            order(200,295)
                     /site_type="other"
                     /note="catalytic triad"
                     /db_xref="CDD:238382"
     CDS             1..375
                     /gene="LOC144115320"
                     /coded_by="XM_077649654.1:2286..3413"
                     /db_xref="GeneID:144115320"
ORIGIN      
        1 mvttniclvl llllhcksys taeeqtrirs eppealkakl nqteersaga ekvcyanvgc
       61 flrrdrltlp sqfpgnpnlf pprfllysrs qghyptivql kpkenyrylr lfkktkplyi
      121 lihgwlesay tpwaqaikda llqeadcnvm vvdwsglaat tyagaaanta avgrtlsllv
      181 qrlfrafplr aalvhaigys fgaqvagffg rnlrrdtgsr iaritaldpa gplfndtnvc
      241 vcsedaafvd vihtsggylh qpwqlgllrp tghvdfyvng aknqpgcygs slcdhqrapn
      301 lfleslvnka crlesqpctg gyaaflrhqc grspaaggrs gemgffcpra agrgvqlvtt
      361 aagplrhfci kkrka
//
LOCUS       XP_077505781             375 aa            linear   INV 25-NOV-2025
DEFINITION  putative phospholipase A1 magnifin [Amblyomma americanum].
ACCESSION   XP_077505781
VERSION     XP_077505781.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649655.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..375
                     /product="putative phospholipase A1 magnifin"
                     /calculated_mol_wt=41123
     Region          83..362
                     /region_name="Abhydrolase"
                     /note="alpha/beta hydrolases; cl21494"
                     /db_xref="CDD:473884"
     Site            198..202
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238382"
     Site            order(200,295)
                     /site_type="other"
                     /note="catalytic triad"
                     /db_xref="CDD:238382"
     CDS             1..375
                     /gene="LOC144115320"
                     /coded_by="XM_077649655.1:270..1397"
                     /db_xref="GeneID:144115320"
ORIGIN      
        1 mvttniclvl llllhcksys taeeqtrirs eppealkakl nqteersaga ekvcyanvgc
       61 flrrdrltlp sqfpgnpnlf pprfllysrs qghyptivql kpkenyrylr lfkktkplyi
      121 lihgwlesay tpwaqaikda llqeadcnvm vvdwsglaat tyagaaanta avgrtlsllv
      181 qrlfrafplr aalvhaigys fgaqvagffg rnlrrdtgsr iaritaldpa gplfndtnvc
      241 vcsedaafvd vihtsggylh qpwqlgllrp tghvdfyvng aknqpgcygs slcdhqrapn
      301 lfleslvnka crlesqpctg gyaaflrhqc grspaaggrs gemgffcpra agrgvqlvtt
      361 aagplrhfci kkrka
//
LOCUS       XP_077505782             375 aa            linear   INV 25-NOV-2025
DEFINITION  putative phospholipase A1 magnifin [Amblyomma americanum].
ACCESSION   XP_077505782
VERSION     XP_077505782.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649656.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..375
                     /product="putative phospholipase A1 magnifin"
                     /calculated_mol_wt=41123
     Region          83..362
                     /region_name="Abhydrolase"
                     /note="alpha/beta hydrolases; cl21494"
                     /db_xref="CDD:473884"
     Site            198..202
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238382"
     Site            order(200,295)
                     /site_type="other"
                     /note="catalytic triad"
                     /db_xref="CDD:238382"
     CDS             1..375
                     /gene="LOC144115320"
                     /coded_by="XM_077649656.1:272..1399"
                     /db_xref="GeneID:144115320"
ORIGIN      
        1 mvttniclvl llllhcksys taeeqtrirs eppealkakl nqteersaga ekvcyanvgc
       61 flrrdrltlp sqfpgnpnlf pprfllysrs qghyptivql kpkenyrylr lfkktkplyi
      121 lihgwlesay tpwaqaikda llqeadcnvm vvdwsglaat tyagaaanta avgrtlsllv
      181 qrlfrafplr aalvhaigys fgaqvagffg rnlrrdtgsr iaritaldpa gplfndtnvc
      241 vcsedaafvd vihtsggylh qpwqlgllrp tghvdfyvng aknqpgcygs slcdhqrapn
      301 lfleslvnka crlesqpctg gyaaflrhqc grspaaggrs gemgffcpra agrgvqlvtt
      361 aagplrhfci kkrka
//
LOCUS       XP_077505783             581 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115321 [Amblyomma americanum].
ACCESSION   XP_077505783
VERSION     XP_077505783.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649657.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..581
                     /product="uncharacterized protein LOC144115321"
                     /calculated_mol_wt=64091
     Region          119..369
                     /region_name="ZP"
                     /note="Zona pellucida (ZP) domain; smart00241"
                     /db_xref="CDD:214579"
     CDS             1..581
                     /gene="LOC144115321"
                     /coded_by="XM_077649657.1:660..2405"
                     /db_xref="GeneID:144115321"
ORIGIN      
        1 mprplcllcl llvacawplq qqqvrakeid kaqdlqqeet ahipaehrgg gyeppeppqq
       61 rphheasagh vaavpvipih dghhhgdhha vaavapqdpg dpwplgrpdm pqikslnvkc
      121 eknhmkvfle fdrpfhgmif skghysdpkc vhlppntghi avnfdiflgs cgmsssqqgg
      181 ydnlgsgvfi edtiivqydp qvqeiwdqar rlrctwydfy ekavtfrpfn vdmldavtan
      241 flgdniqcwm qiqvgkgpwa sevagivkig qtmtmvlaik ddenkfdmlv rnciahdgkh
      301 qpivlvdeyg cvarpkimsr fqkiknfgas asvvsyayfq afkfpdsmnv hfqcviqvcr
      361 yecpepkcdg daalgghylp aprdpssays eqkahfvkps esqkqtvsvt kkqgavtvst
      421 dapkenpppv fynrqgeqlp sdndvvhsnt gyaiahtggm prshdarrrr revgadatdi
      481 qtqkviqvva pgdvafnlpt gdndtlvdvy ssraasadgn icislpgfaa glivllllli
      541 vsmlvasflf vrvrhiansk shdqiisyds pefvkvaitp q
//
LOCUS       XP_077505784             406 aa            linear   INV 25-NOV-2025
DEFINITION  WD repeat and FYVE domain containing 2 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505784
VERSION     XP_077505784.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649658.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..406
                     /product="WD repeat and FYVE domain containing 2 isoform
                     X1"
                     /calculated_mol_wt=45665
     Region          <11..280
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          34..71
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          79..116
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          124..160
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          165..203
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          209..233
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          253..282
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          286..355
                     /region_name="FYVE_WDFY1_like"
                     /note="FYVE domain found in WD40 repeat and FYVE
                     domain-containing protein WDFY1 and WDFY2, and similar
                     proteins; cd15718"
                     /db_xref="CDD:277258"
     Site            order(288,292,317..322,324..325,349..351)
                     /site_type="other"
                     /note="putative phosphatidylinositol 3-phosphate binding
                     site [chemical binding]"
                     /db_xref="CDD:277258"
     CDS             1..406
                     /gene="Wdfy2"
                     /coded_by="XM_077649658.1:186..1406"
                     /db_xref="GeneID:144115322"
ORIGIN      
        1 maaeikpivg pdgqvsnakk pqlinklegh qepvnvavii pgeegvisis ddrtvrvwlr
       61 rdtgqywpsi chnmpsaasa mdynsetrrl figmdngsis efqisddfnk mthqknylah
      121 qgrvmgivfs liaewvlsva rdkhflwhcs etgrrlgafq sgawcmalqf daqskhafvg
      181 dysgqitmlk veqtgykpvt tlkghsgsir cltwdaerkl lfsggfdqti ivwdiggkqg
      241 tayelqghhn kvtalcyhsp gktlisaged smlvfwnmtt krietpewge sdtcqrcsrp
      301 ffwnikamvd qktigirqhh crkcgkavcd kcssnrscip imgyefdvrv cdechvvitd
      361 ddrtsmatfh dakhaivhmh ldltrghllt tgtdrimkiw dishml
//
LOCUS       XP_077505785             333 aa            linear   INV 25-NOV-2025
DEFINITION  WD repeat and FYVE domain containing 2 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505785
VERSION     XP_077505785.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649659.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..333
                     /product="WD repeat and FYVE domain containing 2 isoform
                     X2"
                     /calculated_mol_wt=37611
     Region          <47..207
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          93..131
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          136..174
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          180..209
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          213..282
                     /region_name="FYVE_WDFY1_like"
                     /note="FYVE domain found in WD40 repeat and FYVE
                     domain-containing protein WDFY1 and WDFY2, and similar
                     proteins; cd15718"
                     /db_xref="CDD:277258"
     Site            order(215,219,244..249,251..252,276..278)
                     /site_type="other"
                     /note="putative phosphatidylinositol 3-phosphate binding
                     site [chemical binding]"
                     /db_xref="CDD:277258"
     CDS             1..333
                     /gene="Wdfy2"
                     /coded_by="XM_077649659.1:3578..4579"
                     /db_xref="GeneID:144115322"
ORIGIN      
        1 mpsaasamdy nsetrrlfig mdngsisefq isddfnkmth qknylahqgr vmgivfslia
       61 ewvlsvardk hflwhcsetg rrlgafqsga wcmalqfdaq skhafvgdys gqitmlkveq
      121 tgykpvttlk ghsgsirclt wdaerkllfs ggfdqtiivw diggkqgtay elqghhnkvt
      181 alcyhspgkt lisagedsml vfwnmttkri etpewgesdt cqrcsrpffw nikamvdqkt
      241 igirqhhcrk cgkavcdkcs snrscipimg yefdvrvcde chvvitdddr tsmatfhdak
      301 haivhmhldl trghllttgt drimkiwdis hml
//
LOCUS       XP_077505786             323 aa            linear   INV 25-NOV-2025
DEFINITION  dehydrogenase/reductase SDR family member 12-like [Amblyomma
            americanum].
ACCESSION   XP_077505786
VERSION     XP_077505786.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649660.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..323
                     /product="dehydrogenase/reductase SDR family member
                     12-like"
                     /calculated_mol_wt=36064
     Region          41..296
                     /region_name="NADB_Rossmann"
                     /note="Rossmann-fold NAD(P)(+)-binding proteins; cl21454"
                     /db_xref="CDD:473865"
     Site            order(48,50..51,53,72..74,128..130,182..184,205,209,
                     233..236)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:187535"
     Site            order(150,184,205,209)
                     /site_type="active"
                     /db_xref="CDD:187535"
     CDS             1..323
                     /gene="LOC144115323"
                     /coded_by="XM_077649660.1:204..1175"
                     /db_xref="GeneID:144115323"
ORIGIN      
        1 mtsfyrnvfw fakgmkeysr sgfrsacrsf nlpdldvdct gkifmitgat gtlgkvvaie
       61 laqkggtvhl vcrdeekaea lkksivdite nesavvvhil dmsnpqdvfn fakrfkenna
      121 ilhvlvncas titqnreige gglektfatn tlgvhilitn lipllnqsce prvvlvtcgs
      181 llmqrldpvd lqfecmfpfn gltaftqtkr hqavlvehya etypgihfsv mhpgwlepeg
      241 vkeavpkllr kvsvrcrsve eaadtilwla israalkhss gmffqdrrtt cphipfgktk
      301 tsiddekffm knledfvnkl gls
//
LOCUS       XP_077505787             522 aa            linear   INV 25-NOV-2025
DEFINITION  sterol O-acyltransferase 1 [Amblyomma americanum].
ACCESSION   XP_077505787
VERSION     XP_077505787.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649661.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..522
                     /product="sterol O-acyltransferase 1"
                     /calculated_mol_wt=59871
     Region          <199..427
                     /region_name="MBOAT"
                     /note="membrane-bound O-acyltransferase family; cl00738"
                     /db_xref="CDD:445070"
     CDS             1..522
                     /gene="LOC144115325"
                     /coded_by="XM_077649661.1:613..2181"
                     /db_xref="GeneID:144115325"
ORIGIN      
        1 mepsgsaeql lglktsierl rhsvnveldn lsatldtlar kekdrnnqah kptteaqlrk
       61 ksspgqdlpe kffairesri veimanehir tvfnifvafl flvtvilits yfydpqrlsd
      121 dmstivvnst gmlgvfgyvl ainlailflv hpslqlwahc rslgksgpvd avfaslyilf
      181 lgcviafpvr yifvnelppl sscilafeqv rllmkvyayt reiigrvlah aqlkaksenn
      241 hveltmpgls cvvyflfapt lvyrdsyprt pkvrwgavlw ysgqflacva mysvvlnhfi
      301 kdlfcdagke gfqllgftlt gcaimllgsi smflvfygfl hcwlnifaea mrfgdrlfyl
      361 dwwnsttyad yyrawnlvvh dwlfnyvyrd aykafnynkk ramlvvffls alvheyilal
      421 syrfffpvvl viygtlgval mfatkkktar fwnvflwitl iagwgiilif ytvefysrln
      481 cpttksavad ffiprsfsws cnsfshsswf sssfykqkps lt
//
LOCUS       XP_077505788             522 aa            linear   INV 25-NOV-2025
DEFINITION  sterol O-acyltransferase 1 [Amblyomma americanum].
ACCESSION   XP_077505788
VERSION     XP_077505788.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649662.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..522
                     /product="sterol O-acyltransferase 1"
                     /calculated_mol_wt=59871
     Region          <199..427
                     /region_name="MBOAT"
                     /note="membrane-bound O-acyltransferase family; cl00738"
                     /db_xref="CDD:445070"
     CDS             1..522
                     /gene="LOC144115325"
                     /coded_by="XM_077649662.1:300..1868"
                     /db_xref="GeneID:144115325"
ORIGIN      
        1 mepsgsaeql lglktsierl rhsvnveldn lsatldtlar kekdrnnqah kptteaqlrk
       61 ksspgqdlpe kffairesri veimanehir tvfnifvafl flvtvilits yfydpqrlsd
      121 dmstivvnst gmlgvfgyvl ainlailflv hpslqlwahc rslgksgpvd avfaslyilf
      181 lgcviafpvr yifvnelppl sscilafeqv rllmkvyayt reiigrvlah aqlkaksenn
      241 hveltmpgls cvvyflfapt lvyrdsyprt pkvrwgavlw ysgqflacva mysvvlnhfi
      301 kdlfcdagke gfqllgftlt gcaimllgsi smflvfygfl hcwlnifaea mrfgdrlfyl
      361 dwwnsttyad yyrawnlvvh dwlfnyvyrd aykafnynkk ramlvvffls alvheyilal
      421 syrfffpvvl viygtlgval mfatkkktar fwnvflwitl iagwgiilif ytvefysrln
      481 cpttksavad ffiprsfsws cnsfshsswf sssfykqkps lt
//
LOCUS       XP_077505789             319 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115327 [Amblyomma americanum].
ACCESSION   XP_077505789
VERSION     XP_077505789.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649663.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..319
                     /product="uncharacterized protein LOC144115327"
                     /calculated_mol_wt=32705
     CDS             1..319
                     /gene="LOC144115327"
                     /coded_by="XM_077649663.1:473..1432"
                     /db_xref="GeneID:144115327"
ORIGIN      
        1 mskdgatrgr tnghkqerlt qrshlarvpt agnhgpsgrs qaapsalpln gttqrsfaqv
       61 vqervvtaap pqlrsrtanv tthgegttsn gartvaptkg sptnlplpta tpppllrsrt
      121 anvttrgegt tshgartvap tkgsptnlps ptaapppklr srrtanvttr gegtishgar
      181 tvaptkgspn nlplptaapp pklrsrrtan vttrgegtis hgartvaptk gsptnlpspt
      241 aapppklrsr rtanvttrge gtishgartv aptkgsptnl lsptaapppk rrsrrtanvt
      301 trgegttsng artvaptkg
//
LOCUS       XP_077505790            1338 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505790
VERSION     XP_077505790.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649664.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1338
                     /product="protein argonaute-2-like isoform X1"
                     /calculated_mol_wt=147472
     Region          <6..420
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          573..642
                     /region_name="ArgoN"
                     /note="N-terminal domain of argonaute; pfam16486"
                     /db_xref="CDD:465134"
     Region          652..703
                     /region_name="ArgoL1"
                     /note="Argonaute linker 1 domain; pfam08699"
                     /db_xref="CDD:462567"
     Region          738..839
                     /region_name="PAZ"
                     /note="PAZ domain; pfam02170"
                     /db_xref="CDD:460472"
     Region          868..1294
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(998,1002,1014..1017,1020,1029,1032,1036,1040)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(1067,1069,1145,1285)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..1338
                     /gene="LOC144115329"
                     /coded_by="XM_077649664.1:461..4477"
                     /db_xref="GeneID:144115329"
ORIGIN      
        1 mskdgatrgr tnghkqerlt qrshlarvpt agnhgpsgrs qaapsalpln gtaqrsfaqv
       61 vqervvtaap pqlrsrtanv tthgegttsn gartvaptkg sptnlplpta apppllrsrt
      121 anvttrgegt tshgartvap tkgsptnlts ptaapppklr srrtanvttr gegttshgar
      181 tvaptkgspt nltsptaapp pklrsrrtan vttrgegtis hgartvaptk gspnnlplpt
      241 aapppllrsr tanvttrgeg ttshgartva ptkgsptnlp sptaapppqr rsrrtanvtt
      301 rgegttsnra rtvaptkgsp tnllsptaap ppkrrsrrta nvttrgegtt sngartvapt
      361 nlpsptaapp pqrrsrrtan vttrgegtts nrartvaptk gsptnlpspt aapppkrrsr
      421 rtanvttrge gttsnrartv aptkgsptnl paptaapvea vqarngvgna klqndelkiq
      481 eieralpshf prrpaqgtlg rsiqlianyf sveipsgnff hydveicskt qkkakvpekr
      541 kyrcistkin rlvmellvkk yrvdlngcmp aydgrknlyt rcelkfrert ftvdfeedqr
      601 iqkfivkiqy aatvnldvlh avfdnrisqv pqevlqavdi vmrhgpsikl tpvgrcffqa
      661 psykdyytlg ggrevwfgyy asvrpaqwkp mlnidmsata fyqplpvtnf mckllsedcc
      721 emsvkdfrel hdsqivrlnk elkglrikvt hlpyprkykv vcvtkesakk lffnmedgsr
      781 csvadyfqnr yrrlsypnlp ciqtgsttrp vylplevcai vegqhckkkl denqtsemik
      841 rtaqppamrf nqihrtvrdv vscseqrlhe feinisteat qlrgrvldpp slvfennsig
      901 kpregtwelr grhfykpatl trwtllnlsr fveqdsldnf vkmlirvsqe lgmrieqpld
      961 vtttdasrkl vrnilleeqr ktaslemvii vltkntnyae ikqvaeteig lrtqcvmdnn
     1021 vikkcntali tnlcqkinak ldginnsllp kempkmfekp viiigadvth papeenlrps
     1081 iaacvgslda ipskfhasir iqmedsaats rleiimdlkg mmkemlvafy ratrhkpeki
     1141 iffrdgvseg qfmevrnrev sairlacqel spnetyepal tfivvqkrhh trfipandrd
     1201 gvgkcgnvpp gtivdsvvth pldfdfylcs hfgiqgtsrp shyyivwdds sltadelqkl
     1261 cyylchtyar csrsvsipap vyyahlaayr arnhvmskvd vsssssdssg ggansistte
     1321 yveavnvhds lktamyfv
//
LOCUS       XP_077505791            1298 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505791
VERSION     XP_077505791.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649665.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1298
                     /product="protein argonaute-2-like isoform X2"
                     /calculated_mol_wt=143182
     Region          <1..380
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          533..602
                     /region_name="ArgoN"
                     /note="N-terminal domain of argonaute; pfam16486"
                     /db_xref="CDD:465134"
     Region          612..663
                     /region_name="ArgoL1"
                     /note="Argonaute linker 1 domain; pfam08699"
                     /db_xref="CDD:462567"
     Region          698..799
                     /region_name="PAZ"
                     /note="PAZ domain; pfam02170"
                     /db_xref="CDD:460472"
     Region          828..1254
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(958,962,974..977,980,989,992,996,1000)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(1027,1029,1105,1245)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..1298
                     /gene="LOC144115329"
                     /coded_by="XM_077649665.1:87..3983"
                     /db_xref="GeneID:144115329"
ORIGIN      
        1 maapsalpln gtaqrsfaqv vqervvtaap pqlrsrtanv tthgegttsn gartvaptkg
       61 sptnlplpta apppllrsrt anvttrgegt tshgartvap tkgsptnlts ptaapppklr
      121 srrtanvttr gegttshgar tvaptkgspt nltsptaapp pklrsrrtan vttrgegtis
      181 hgartvaptk gspnnlplpt aapppllrsr tanvttrgeg ttshgartva ptkgsptnlp
      241 sptaapppqr rsrrtanvtt rgegttsnra rtvaptkgsp tnllsptaap ppkrrsrrta
      301 nvttrgegtt sngartvapt nlpsptaapp pqrrsrrtan vttrgegtts nrartvaptk
      361 gsptnlpspt aapppkrrsr rtanvttrge gttsnrartv aptkgsptnl paptaapvea
      421 vqarngvgna klqndelkiq eieralpshf prrpaqgtlg rsiqlianyf sveipsgnff
      481 hydveicskt qkkakvpekr kyrcistkin rlvmellvkk yrvdlngcmp aydgrknlyt
      541 rcelkfrert ftvdfeedqr iqkfivkiqy aatvnldvlh avfdnrisqv pqevlqavdi
      601 vmrhgpsikl tpvgrcffqa psykdyytlg ggrevwfgyy asvrpaqwkp mlnidmsata
      661 fyqplpvtnf mckllsedcc emsvkdfrel hdsqivrlnk elkglrikvt hlpyprkykv
      721 vcvtkesakk lffnmedgsr csvadyfqnr yrrlsypnlp ciqtgsttrp vylplevcai
      781 vegqhckkkl denqtsemik rtaqppamrf nqihrtvrdv vscseqrlhe feinisteat
      841 qlrgrvldpp slvfennsig kpregtwelr grhfykpatl trwtllnlsr fveqdsldnf
      901 vkmlirvsqe lgmrieqpld vtttdasrkl vrnilleeqr ktaslemvii vltkntnyae
      961 ikqvaeteig lrtqcvmdnn vikkcntali tnlcqkinak ldginnsllp kempkmfekp
     1021 viiigadvth papeenlrps iaacvgslda ipskfhasir iqmedsaats rleiimdlkg
     1081 mmkemlvafy ratrhkpeki iffrdgvseg qfmevrnrev sairlacqel spnetyepal
     1141 tfivvqkrhh trfipandrd gvgkcgnvpp gtivdsvvth pldfdfylcs hfgiqgtsrp
     1201 shyyivwdds sltadelqkl cyylchtyar csrsvsipap vyyahlaayr arnhvmskvd
     1261 vsssssdssg ggansistte yveavnvhds lktamyfv
//
LOCUS       XP_077505792             424 aa            linear   INV 25-NOV-2025
DEFINITION  histone deacetylase 8-like [Amblyomma americanum].
ACCESSION   XP_077505792
VERSION     XP_077505792.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649666.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..424
                     /product="histone deacetylase 8-like"
                     /calculated_mol_wt=45993
     Region          57..417
                     /region_name="Arginase_HDAC"
                     /note="Arginase-like and histone-like hydrolases; cl17011"
                     /db_xref="CDD:450134"
     Site            order(188,223,225,227,310,312,349)
                     /site_type="active"
                     /db_xref="CDD:212524"
     Site            order(223,225,312)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:212524"
     CDS             1..424
                     /gene="LOC144115330"
                     /coded_by="XM_077649666.1:138..1412"
                     /db_xref="GeneID:144115330"
ORIGIN      
        1 mygsvystlf nnddgddqts qanvsrskls elpssvsavp nvgyehcasg tstksrvgyv
       61 hspghlfece rlprirkrcf lvhgllkgyn llknlhpihp flasredlcl fhsadyvdfl
      121 esceaaedld gedivsraqd ynlvddcapt erlltlvknv aggtlaaara liertcdvai
      181 hweggwhhah rseaagfcyv ndvvlgilal rrkfgrvlyi dldvhhgdgv qeafegtdkv
      241 ltvsvhqyep gffpgtgavn dvgfglgrgy sinlplrsga sdstflravl pvisevrqry
      301 apdavvcqcg adglsgdplg awnltpaafv qcvlviqswg lpllllgggg yeaancarcw
      361 tavtaallgq eldddipehr flmaygpgye lsmepgfrrd lndgqyvdgi lqevmkhvsl
      421 fggs
//
LOCUS       XP_077505793             424 aa            linear   INV 25-NOV-2025
DEFINITION  histone deacetylase 8-like [Amblyomma americanum].
ACCESSION   XP_077505793
VERSION     XP_077505793.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649667.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..424
                     /product="histone deacetylase 8-like"
                     /calculated_mol_wt=45993
     Region          57..417
                     /region_name="Arginase_HDAC"
                     /note="Arginase-like and histone-like hydrolases; cl17011"
                     /db_xref="CDD:450134"
     Site            order(188,223,225,227,310,312,349)
                     /site_type="active"
                     /db_xref="CDD:212524"
     Site            order(223,225,312)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:212524"
     CDS             1..424
                     /gene="LOC144115330"
                     /coded_by="XM_077649667.1:143..1417"
                     /db_xref="GeneID:144115330"
ORIGIN      
        1 mygsvystlf nnddgddqts qanvsrskls elpssvsavp nvgyehcasg tstksrvgyv
       61 hspghlfece rlprirkrcf lvhgllkgyn llknlhpihp flasredlcl fhsadyvdfl
      121 esceaaedld gedivsraqd ynlvddcapt erlltlvknv aggtlaaara liertcdvai
      181 hweggwhhah rseaagfcyv ndvvlgilal rrkfgrvlyi dldvhhgdgv qeafegtdkv
      241 ltvsvhqyep gffpgtgavn dvgfglgrgy sinlplrsga sdstflravl pvisevrqry
      301 apdavvcqcg adglsgdplg awnltpaafv qcvlviqswg lpllllgggg yeaancarcw
      361 tavtaallgq eldddipehr flmaygpgye lsmepgfrrd lndgqyvdgi lqevmkhvsl
      421 fggs
//
LOCUS       XP_077505794             308 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115332 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505794
VERSION     XP_077505794.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649668.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..308
                     /product="uncharacterized protein LOC144115332 isoform X1"
                     /calculated_mol_wt=34020
     CDS             1..308
                     /gene="LOC144115332"
                     /coded_by="XM_077649668.1:165..1091"
                     /db_xref="GeneID:144115332"
ORIGIN      
        1 miicncrgmg sclsycckeq skrarhvkpa gyfdssslie fetlideqss sepkslqsle
       61 lpltechapf etwcnckcsa qlrlyhlnfe epstsddelm tlsgisddda iiteklafhq
      121 edaleleeea kmqqpdsvev ppasvgqrvv mrsrlskedn wtgadhsawa lageeeilnv
      181 trlqcqghlp sllavlsgpq fqakaweker erlnssphss ldlewenevg ltppqqcasa
      241 adddfvyvpg adtcsnhstp lsnendlewd gdlssvqigg fdmeteqlis eieqmtadal
      301 kepsgasr
//
LOCUS       XP_077505795             307 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115332 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505795
VERSION     XP_077505795.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649669.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..307
                     /product="uncharacterized protein LOC144115332 isoform X2"
                     /calculated_mol_wt=33892
     CDS             1..307
                     /gene="LOC144115332"
                     /coded_by="XM_077649669.1:166..1089"
                     /db_xref="GeneID:144115332"
ORIGIN      
        1 miicncrgmg sclsycckeq skrarhvkpa gyfdssslie fetlidesss epkslqslel
       61 pltechapfe twcnckcsaq lrlyhlnfee pstsddelmt lsgisdddai iteklafhqe
      121 daleleeeak mqqpdsvevp pasvgqrvvm rsrlskednw tgadhsawal ageeeilnvt
      181 rlqcqghlps llavlsgpqf qakawekere rlnssphssl dlewenevgl tppqqcasaa
      241 dddfvyvpga dtcsnhstpl snendlewdg dlssvqiggf dmeteqlise ieqmtadalk
      301 epsgasr
//
LOCUS       XP_077505796             302 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115332 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505796
VERSION     XP_077505796.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649670.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..302
                     /product="uncharacterized protein LOC144115332 isoform X3"
                     /calculated_mol_wt=33469
     CDS             1..302
                     /gene="LOC144115332"
                     /coded_by="XM_077649670.1:165..1073"
                     /db_xref="GeneID:144115332"
ORIGIN      
        1 miicncrgmg sclsycckeq skrarhvkpa gyfdssslie fetlideqss sepkslqsle
       61 lpltechapf etwcnckcsa qlrlyhlnfe epstsddelm tlsgisddda iiteklafhq
      121 edaleleeea kmqqpdsvev ppasvgqrvv mrsrlskedn wtgadhsawa lageeeilnv
      181 trlqcqghlp scpqfqakaw ekererlnss phssldlewe nevgltppqq casaadddfv
      241 yvpgadtcsn hstplsnend lewdgdlssv qiggfdmete qliseieqmt adalkepsga
      301 sr
//
LOCUS       XP_077505797             301 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115332 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505797
VERSION     XP_077505797.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649671.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..301
                     /product="uncharacterized protein LOC144115332 isoform X4"
                     /calculated_mol_wt=33341
     CDS             1..301
                     /gene="LOC144115332"
                     /coded_by="XM_077649671.1:158..1063"
                     /db_xref="GeneID:144115332"
ORIGIN      
        1 miicncrgmg sclsycckeq skrarhvkpa gyfdssslie fetlidesss epkslqslel
       61 pltechapfe twcnckcsaq lrlyhlnfee pstsddelmt lsgisdddai iteklafhqe
      121 daleleeeak mqqpdsvevp pasvgqrvvm rsrlskednw tgadhsawal ageeeilnvt
      181 rlqcqghlps cpqfqakawe kererlnssp hssldlewen evgltppqqc asaadddfvy
      241 vpgadtcsnh stplsnendl ewdgdlssvq iggfdmeteq liseieqmta dalkepsgas
      301 r
//
LOCUS       XP_077505798             259 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115332 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077505798
VERSION     XP_077505798.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649672.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..259
                     /product="uncharacterized protein LOC144115332 isoform X5"
                     /calculated_mol_wt=28524
     CDS             1..259
                     /gene="LOC144115332"
                     /coded_by="XM_077649672.1:166..945"
                     /db_xref="GeneID:144115332"
ORIGIN      
        1 miicncrgmg sclsycckeq skrarhvkpa gyfdssslie fetlideqss sepksisswl
       61 nfkkgklafh qedaleleee akmqqpdsve vppasvgqrv vmrsrlsked nwtgadhsaw
      121 alageeeiln vtrlqcqghl psllavlsgp qfqakaweke rerlnssphs sldlewenev
      181 gltppqqcas aadddfvyvp gadtcsnhst plsnendlew dgdlssvqig gfdmeteqli
      241 seieqmtada lkepsgasr
//
LOCUS       XP_077505799             258 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115332 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077505799
VERSION     XP_077505799.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649673.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..258
                     /product="uncharacterized protein LOC144115332 isoform X6"
                     /calculated_mol_wt=28396
     CDS             1..258
                     /gene="LOC144115332"
                     /coded_by="XM_077649673.1:167..943"
                     /db_xref="GeneID:144115332"
ORIGIN      
        1 miicncrgmg sclsycckeq skrarhvkpa gyfdssslie fetlidesss epksisswln
       61 fkkgklafhq edaleleeea kmqqpdsvev ppasvgqrvv mrsrlskedn wtgadhsawa
      121 lageeeilnv trlqcqghlp sllavlsgpq fqakaweker erlnssphss ldlewenevg
      181 ltppqqcasa adddfvyvpg adtcsnhstp lsnendlewd gdlssvqigg fdmeteqlis
      241 eieqmtadal kepsgasr
//
LOCUS       XP_077505800             253 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115332 isoform X7 [Amblyomma
            americanum].
ACCESSION   XP_077505800
VERSION     XP_077505800.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649674.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..253
                     /product="uncharacterized protein LOC144115332 isoform X7"
                     /calculated_mol_wt=27973
     CDS             1..253
                     /gene="LOC144115332"
                     /coded_by="XM_077649674.1:159..920"
                     /db_xref="GeneID:144115332"
ORIGIN      
        1 miicncrgmg sclsycckeq skrarhvkpa gyfdssslie fetlideqss sepksisswl
       61 nfkkgklafh qedaleleee akmqqpdsve vppasvgqrv vmrsrlsked nwtgadhsaw
      121 alageeeiln vtrlqcqghl pscpqfqaka wekererlns sphssldlew enevgltppq
      181 qcasaadddf vyvpgadtcs nhstplsnen dlewdgdlss vqiggfdmet eqliseieqm
      241 tadalkepsg asr
//
LOCUS       XP_077505801             289 aa            linear   INV 25-NOV-2025
DEFINITION  secreted protein, acidic, cysteine-rich [Amblyomma americanum].
ACCESSION   XP_077505801
VERSION     XP_077505801.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649675.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..289
                     /product="secreted protein, acidic, cysteine-rich"
                     /calculated_mol_wt=33003
     Region          67..159
                     /region_name="KAZAL_FS"
                     /note="Kazal type serine protease inhibitors and
                     follistatin-like domains. Kazal inhibitors inhibit serine
                     proteases, such as, trypsin, chyomotrypsin, avian
                     ovomucoids, and elastases. The inhibitory domain has one
                     reactive site peptide bond, which serves the...; cl00097"
                     /db_xref="CDD:412159"
     Region          162..277
                     /region_name="EFh_SPARC_like"
                     /note="EF-hand, extracellular calcium-binding (EC) motif,
                     found in secreted protein acidic and rich in cysteine
                     (SPARC) and similar proteins; cd16231"
                     /db_xref="CDD:320010"
     Site            order(169..170,173..174,176..177,180..181,184,245..246)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:320010"
     Region          210..243
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320010"
     Site            order(222,227,234,257,259,261,268)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320010"
     Site            order(232..233,247)
                     /site_type="other"
                     /note="EC/FS-domain interface"
                     /db_xref="CDD:320010"
     Region          245..277
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320010"
     CDS             1..289
                     /gene="SPARC"
                     /coded_by="XM_077649675.1:164..1033"
                     /db_xref="GeneID:144115333"
ORIGIN      
        1 mkafvillll glvlaaeakk hhgskhgkkh htkkakdavd iaaedekasi lkdksaelda
       61 ervlkdpcsk vrcgpgreci inedltatce cvskcqpead prrkasessv vcasnhnqtw
      121 esdcelyqmr clcedekegc kgekyehvhi dyygacrelp kcldeemedf prrmrewlfn
      181 vmqdlarrhe lnepykklee eaenlqsrqw vnaviwkfce ldshphdrav srhelfplra
      241 pllsmehcia pflnacdkdd dhtitlkewg dclgledgev qdrcaqita
//
LOCUS       XP_077505802             368 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115334 [Amblyomma americanum].
ACCESSION   XP_077505802
VERSION     XP_077505802.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649676.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 20% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..368
                     /product="uncharacterized protein LOC144115334"
                     /calculated_mol_wt=41507
     CDS             1..368
                     /gene="LOC144115334"
                     /coded_by="XM_077649676.1:1..1107"
                     /db_xref="GeneID:144115334"
ORIGIN      
        1 msdlpmdght llrtprkpqe qcpvtplapg hycyfgladg llrllrlven ppaliplsfn
       61 idglplskss kmqlwpiqcl ahdcgdetpf lvgafagksk pdssveflrp fvselrtlls
      121 eglifngrvl hvimayaive flhheqeevv plswiddrqc awpdhakgdr ivslvrkaap
      181 pdsswkrfdi alkglfgayk lgrrkldksq ytselssdge pphkrtrrpp qawsedevsd
      241 tpttkvpyre nvpsvphdfp qgdfqelvri gdkdlfdkgh egearlsdrr dsvdedfkqh
      301 virllnilrf tqqqhgdmln klcevlpsqa iitkpllise pftsvddllk fnekldkenc
      361 tsglltkt
//
LOCUS       XP_077505803             536 aa            linear   INV 25-NOV-2025
DEFINITION  poly(A) RNA polymerase, mitochondrial-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505803
VERSION     XP_077505803.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649677.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..536
                     /product="poly(A) RNA polymerase, mitochondrial-like
                     isoform X1"
                     /calculated_mol_wt=60743
     Region          165..>468
                     /region_name="TRF4"
                     /note="DNA polymerase sigma [Replication, recombination
                     and repair]; COG5260"
                     /db_xref="CDD:227585"
     Region          190..319
                     /region_name="NT_PAP_TUTase"
                     /note="Nucleotidyltransferase (NT) domain of poly(A)
                     polymerases and terminal uridylyl transferases; cd05402"
                     /db_xref="CDD:143392"
     Site            order(212..214,224..225,227,285..286,288,290,301,308..309,
                     312..313)
                     /site_type="active"
                     /db_xref="CDD:143392"
     Site            order(212,285,288,290,301,308..309)
                     /site_type="active"
                     /note="putative primer-binding pocket [active]"
                     /db_xref="CDD:143392"
     Site            order(225,227,301)
                     /site_type="metal-binding"
                     /note="metal binding triad [ion binding]"
                     /db_xref="CDD:143392"
     CDS             1..536
                     /gene="LOC144115335"
                     /coded_by="XM_077649677.1:61..1671"
                     /db_xref="GeneID:144115335"
ORIGIN      
        1 mscfrrkhll lrcrtallgr hvqheirkrl lcsqineqav psfdtilsqr rkessasvlv
       61 evenrhktae lcnecslygk iqkayyytdn shkcqvlief sssdealell qkcghlssdg
      121 fpmrsrllkf vpknrtratk kslsvdiets snitpqaqgt klvqsntide qmvhfyetek
      181 lsdletrlgf ivckqveefi vglypkgqvl pfgslvngfg rhycdidmvy cvseatepsg
      241 nlyfqeknqv indrtlvqrl letlgdllhy vvpgvsevqr ilrarvpivk fqhnivgrec
      301 dltlsnmsgv hmsrllytct qlapalcpll ftarswamaq gvtskvpgtw itnfqltlla
      361 ifhlqqcgll pslrdleekk rlkawqksrv ldgkdealed llksfyefya sfnfkskgia
      421 pysgqilekp eytamyiqnp ldrqlnasrn vglsdlkklq srmadaltlm dnaprkitie
      481 kpwgimalft attaaakrqq eamhkrfqvh elfhegkeag enyvveatas giktas
//
LOCUS       XP_077505804             518 aa            linear   INV 25-NOV-2025
DEFINITION  poly(A) RNA polymerase, mitochondrial-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505804
VERSION     XP_077505804.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649678.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..518
                     /product="poly(A) RNA polymerase, mitochondrial-like
                     isoform X2"
                     /calculated_mol_wt=58401
     Region          147..>450
                     /region_name="TRF4"
                     /note="DNA polymerase sigma [Replication, recombination
                     and repair]; COG5260"
                     /db_xref="CDD:227585"
     Region          172..301
                     /region_name="NT_PAP_TUTase"
                     /note="Nucleotidyltransferase (NT) domain of poly(A)
                     polymerases and terminal uridylyl transferases; cd05402"
                     /db_xref="CDD:143392"
     Site            order(194..196,206..207,209,267..268,270,272,283,290..291,
                     294..295)
                     /site_type="active"
                     /db_xref="CDD:143392"
     Site            order(194,267,270,272,283,290..291)
                     /site_type="active"
                     /note="putative primer-binding pocket [active]"
                     /db_xref="CDD:143392"
     Site            order(207,209,283)
                     /site_type="metal-binding"
                     /note="metal binding triad [ion binding]"
                     /db_xref="CDD:143392"
     CDS             1..518
                     /gene="LOC144115335"
                     /coded_by="XM_077649678.1:696..2252"
                     /db_xref="GeneID:144115335"
ORIGIN      
        1 maqallsrrt tqlvteineq avpsfdtils qrrkessasv lvevenrhkt aelcnecsly
       61 gkiqkayyyt dnshkcqvli efsssdeale llqkcghlss dgfpmrsrll kfvpknrtra
      121 tkkslsvdie tssnitpqaq gtklvqsnti deqmvhfyet eklsdletrl gfivckqvee
      181 fivglypkgq vlpfgslvng fgrhycdidm vycvseatep sgnlyfqekn qvindrtlvq
      241 rlletlgdll hyvvpgvsev qrilrarvpi vkfqhnivgr ecdltlsnms gvhmsrllyt
      301 ctqlapalcp llftarswam aqgvtskvpg twitnfqltl laifhlqqcg llpslrdlee
      361 kkrlkawqks rvldgkdeal edllksfyef yasfnfkskg iapysgqile kpeytamyiq
      421 npldrqlnas rnvglsdlkk lqsrmadalt lmdnaprkit iekpwgimal ftattaaakr
      481 qqeamhkrfq vhelfhegke agenyvveat asgiktas
//
LOCUS       XP_077505805             173 aa            linear   INV 25-NOV-2025
DEFINITION  translation machinery-associated protein 16 [Amblyomma americanum].
ACCESSION   XP_077505805
VERSION     XP_077505805.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649679.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..173
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..173
                     /product="translation machinery-associated protein 16"
                     /calculated_mol_wt=19682
     Region          21..169
                     /region_name="Tma16"
                     /note="Translation machinery-associated protein 16;
                     pfam11176"
                     /db_xref="CDD:463236"
     CDS             1..173
                     /gene="LOC144115336"
                     /coded_by="XM_077649679.1:171..692"
                     /db_xref="GeneID:144115336"
ORIGIN      
        1 mpkanqaekk rriqartsrp vhpnsrkaqq markkihkdk vtarkkdlal klktklqkla
       61 wfrenladvv ptgpltpsdl galiekyfqr fsseiehvnn iqqirgnvtq fsgrldaikm
      121 tldkeigdyt scgievpdll spesfksfie wdgqdvsylp kvtmrvfska mvq
//
LOCUS       XP_077505806            1423 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115337 [Amblyomma americanum].
ACCESSION   XP_077505806
VERSION     XP_077505806.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649680.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1423
                     /product="uncharacterized protein LOC144115337"
                     /calculated_mol_wt=156186
     Region          1264..1407
                     /region_name="MATH"
                     /note="MATH (meprin and TRAF-C homology) domain; an
                     independent folding unit with an eight-stranded
                     beta-sandwich structure found in meprins, TRAFs and other
                     proteins. Meprins comprise a class of extracellular
                     metalloproteases which are anchored to the membrane...;
                     cl02446"
                     /db_xref="CDD:445786"
     Site            order(1319,1378..1380)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:239745"
     CDS             1..1423
                     /gene="LOC144115337"
                     /coded_by="XM_077649680.1:173..4444"
                     /db_xref="GeneID:144115337"
ORIGIN      
        1 manrklytlf gfcpeldwrp tafvgpippd rvctscgrip msglllpcqd lvclpcferd
       61 etcpvhetaf peediqtttf slqhmmqrkv ycwnsskgcd avgalsfmld hhekncpfgs
      121 apahaeksta elgqhhsnpe rltvprreqr petvlkevcg vtprkerpse dsgvaslsdf
      181 vvkndetsst tshsitvsth agrlserlhs svriqvdtrs trdtaareaa esrsrkisat
      241 rapqqrtyep trsafenvle hrrsstaepl nsnlpndaeq yqrrrtrsnn dsasnesaaf
      301 laprraasta prskpqqggt ssrisesmgm ratealetvs nsakppgdas aegailennp
      361 nepflsrqfq eaelcqssdv lgikregrsg yteailpgsc gstqrplgeg psgttvtslv
      421 sddsrmsheg sreneppdnn alraldhhat dttnerneyd isqssrases sgeyeesled
      481 tessedfsst aqeqetgyqg crgvpaapse dtrntaeddt kperphdcev vpqatscngt
      541 aqdrpvrvea asartplkkv sdqikyivsg pffrapvaen erdrtledya esfshknkgs
      601 apeahfkgel fapdkaesaa leslertlae rrkavdirmm ggrvecselg sgvpsttrql
      661 peigvsecek rtecldcsts halrsvtdpp gpcretersa iakeattndp vlssgqetak
      721 glhsellrre hgghnatpvl vdgheafssv epindhlgsl lkdtsekipq qglgvgissn
      781 sktrelldfs vfesrkllpf hqrgqhykkg kdfcsastns dnsvpsltvs ettvsgpeve
      841 qpenlgtnfp psgshsvver ngsqlsnhad eektseghry leqnsshhls keafgklnts
      901 slqsatgcvg igkafldses mtdnvnfsam lsklneaiei vskgkqfprg ddesrstssd
      961 eemmqesvkk fvyadalnsq snhrgadqhi qlmrenrcse tasagdseat hsacpqnagq
     1021 esnrvpsked tveevekkdr epsrasicdn rlspsvesvt rsseaplsss fsrkstmcct
     1081 qeessctekn sgarkvvnte vqssqlvrrs vtfaeaglsv gggmeqteip aafgtpctqs
     1141 senyaaagli rekageaatt cqdlkpcrrt seneqdtnlr seispmvesp seckeiktey
     1201 lkalmttklg ldavltatke tlghhsknta vlqklqeeir srtcdvvnvc kamlhssfgn
     1261 resfewyinn cdevknrvmq inylslgvss phyfcgynir psvflrkrkg iveihpeltl
     1321 hkgvyddfis wpfegkfrfv ivhpsdated vavdmddhdn gsvpfafrkp kamrniavya
     1381 qryhsfqdie nrgfvcdnrl rlrfevfsss qhcpgissca sqa
//
LOCUS       XP_077505807             171 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115339 [Amblyomma americanum].
ACCESSION   XP_077505807
VERSION     XP_077505807.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649681.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..171
                     /product="uncharacterized protein LOC144115339"
                     /calculated_mol_wt=18597
     CDS             1..171
                     /gene="LOC144115339"
                     /coded_by="XM_077649681.1:50..565"
                     /db_xref="GeneID:144115339"
ORIGIN      
        1 mmrrgaifvh psfpdrpttq rhptgsglql arkddlaqea qcchersrni atrvtrpack
       61 ppvaevakrf vayvrrlgfa fgeqgadhyy hhdyqkglld sekrvcgnap iraqpsavpp
      121 atgtpttqrs npfpveqdid lgpphrslcg adtagsspgf hgrpcmtaet l
//
LOCUS       XP_077505808             214 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115341 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505808
VERSION     XP_077505808.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649682.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..214
                     /product="uncharacterized protein LOC144115341 isoform X1"
                     /calculated_mol_wt=22911
     CDS             1..214
                     /gene="LOC144115341"
                     /coded_by="XM_077649682.1:757..1401"
                     /db_xref="GeneID:144115341"
ORIGIN      
        1 maspceedir aaaasaaaae adesldatlv angseasqsd dsvsvesaps ssatmtpeka
       61 tkdrslsdgr laraaepdvp vrgykaiakh sfaimasvva qwlscrpadl kdealvrrle
      121 drindrtrhl ddhlrhahiq ltlmqrtfiv gapclsnpar acqiecggvc lgsclgetsg
      181 yisylhtfll sseklaifln fgrqwtasaf rded
//
LOCUS       XP_077505809             206 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115341 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505809
VERSION     XP_077505809.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649683.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..206
                     /product="uncharacterized protein LOC144115341 isoform X2"
                     /calculated_mol_wt=20727
     Region          <60..>172
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     CDS             1..206
                     /gene="LOC144115341"
                     /coded_by="XM_077649683.1:457..1077"
                     /db_xref="GeneID:144115341"
ORIGIN      
        1 mlrrvlqgfk ksvdiilkdr thlqrpldtc llykqsvpsa sggnqatapa pppagpsrvs
       61 faqalqqgva aspqpravng gptpaaaara qssaqytaap saqpsgsrps ygaaagsakv
      121 aasspwpasa eaavsgsqpa aaalpqslpc tphgavtept peagdqatpg ddvtvkgpcd
      181 srslmnaswa saegnarrrk qfrspe
//
LOCUS       XP_077505810             203 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115341 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505810
VERSION     XP_077505810.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649684.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..203
                     /product="uncharacterized protein LOC144115341 isoform X3"
                     /calculated_mol_wt=21537
     CDS             1..203
                     /gene="LOC144115341"
                     /coded_by="XM_077649684.1:757..1368"
                     /db_xref="GeneID:144115341"
ORIGIN      
        1 maspceedir aaaasaaaae adesldatlv angseasqsd dsvsvesaps ssatmtpeka
       61 tkdrslsdgr laraaepdvp vrgykaiakh sfaimasvva qwlscrpadl kdealvrrle
      121 drindrtrhl ddhlrhahiq ltlmqrtfiv gapclsnpar acqiecggvc lgsclgetsg
      181 yisylhtfll srgnpdhvag cfr
//
LOCUS       XP_077505811             182 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115341 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505811
VERSION     XP_077505811.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649685.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..182
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..182
                     /product="uncharacterized protein LOC144115341 isoform X4"
                     /calculated_mol_wt=19396
     CDS             1..182
                     /gene="LOC144115341"
                     /coded_by="XM_077649685.1:755..1303"
                     /db_xref="GeneID:144115341"
ORIGIN      
        1 maspceedir aaaasaaaae adesldatlv angseasqsd dsvsvesaps ssatmtpeka
       61 tkdrslsdgr laraaepdvp ealvrrledr indrtrhldd hlrhahiqlt lmqrtfivga
      121 pclsnparac qiecggvclg sclgetsgyi sylhtfllss eklaiflnfg rqwtasafrd
      181 ed
//
LOCUS       XP_077505812             177 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115341 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077505812
VERSION     XP_077505812.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649686.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..177
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..177
                     /product="uncharacterized protein LOC144115341 isoform X5"
                     /calculated_mol_wt=19179
     CDS             1..177
                     /gene="LOC144115341"
                     /coded_by="XM_077649686.1:744..1277"
                     /db_xref="GeneID:144115341"
ORIGIN      
        1 maspceedir aaaasaaaae adesldatlv angseasqsd dsvsvesaps ssatmtpeka
       61 tkdrslsdgr laraaepdvp vrgykaiakh sfaimasvva qwlscrpadl kdealvrrle
      121 drindrtrhl ddhlrhahiq ltlmqrtfig eiqtmlqdvf dkvhalraii demrqsv
//
LOCUS       XP_077505813             145 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115341 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077505813
VERSION     XP_077505813.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649687.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..145
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..145
                     /product="uncharacterized protein LOC144115341 isoform X6"
                     /calculated_mol_wt=15663
     CDS             1..145
                     /gene="LOC144115341"
                     /coded_by="XM_077649687.1:734..1171"
                     /db_xref="GeneID:144115341"
ORIGIN      
        1 maspceedir aaaasaaaae adesldatlv angseasqsd dsvsvesaps ssatmtpeka
       61 tkdrslsdgr laraaepdvp ealvrrledr indrtrhldd hlrhahiqlt lmqrtfigei
      121 qtmlqdvfdk vhalraiide mrqsv
//
LOCUS       XP_077505814             487 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115342 [Amblyomma americanum].
ACCESSION   XP_077505814
VERSION     XP_077505814.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649688.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..487
                     /product="uncharacterized protein LOC144115342"
                     /calculated_mol_wt=52574
     CDS             1..487
                     /gene="LOC144115342"
                     /coded_by="XM_077649688.1:398..1861"
                     /db_xref="GeneID:144115342"
ORIGIN      
        1 mpllrlsaar alrendslrf nsccpfcifv nssaacgfvr epamerrtgq kyrtkyaygk
       61 rkrkppipkq krpaatephe rrsapissha dvsseaslpl esatssesgg lalsashdia
      121 vqyrpghssv faaasggavp vqrddgevlp kqkhtvqvhl psesitgses vgpapstsrd
      181 iavqyrpghs svfaagsaga vpvqrddgev fpkqkrtvqv hlpsesmtgs esgdpapsts
      241 rdiavqhrpg qssvfaaasd dampaqrdds elflkqkrtv qvhleprfvt leaaeeqacs
      301 vratlravss terkfgfarq teeesapded eftliqasvm nsiigsllcp kcskkrlslk
      361 hetklglavk lvlscgacgp iashwsskrk segrtfevni ramqaiktig kgptalndfw
      421 atmnvshrgl hqktyqghlk klfkpgaeea aqnifadavl avkdvyskmq psspsnitvv
      481 ydgtwlt
//
LOCUS       XP_077505815             295 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115343 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505815
VERSION     XP_077505815.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649689.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..295
                     /product="uncharacterized protein LOC144115343 isoform X1"
                     /calculated_mol_wt=31785
     CDS             1..295
                     /gene="LOC144115343"
                     /coded_by="XM_077649689.1:122..1009"
                     /db_xref="GeneID:144115343"
ORIGIN      
        1 mlflhrtlpn srcntfftga pmwrvlrgrr pptraqplde qrqllaqqap tkhrglalqw
       61 rhlspkeema veecyrwkpa yfsdetwckl pddikkcygn vhkdrsiker kgfgvkaqqq
      121 aviiqpvfvg aystaivhwq sgkaagshga taassggmra enrtlrhatf tfnlpeygag
      181 eeaetgvimv qrlllplrts epgllgggqh grghkavert aaiaalslla atyarhaggt
      241 dqqelrpsfa tpgaaaasyt gtafstgtlf cssansarar gndgsaavps atgtl
//
LOCUS       XP_077505816             285 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115343 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505816
VERSION     XP_077505816.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649690.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..285
                     /product="uncharacterized protein LOC144115343 isoform X2"
                     /calculated_mol_wt=30505
     CDS             1..285
                     /gene="LOC144115343"
                     /coded_by="XM_077649690.1:7360..8217"
                     /db_xref="GeneID:144115343"
ORIGIN      
        1 mveplrcaga pmwrvlrgrr pptraqplde qrqllaqqap tkhrglalqw rhlspkeema
       61 veecyrwkpa yfsdetwckl pddikkcygn vhkdrsiker kgfgvkaqqq aviiqpvfvg
      121 aystaivhwq sgkaagshga taassggmra enrtlrhatf tfnlpeygag eeaetgvimv
      181 qrlllplrts epgllgggqh grghkavert aaiaalslla atyarhaggt dqqelrpsfa
      241 tpgaaaasyt gtafstgtlf cssansarar gndgsaavps atgtl
//
LOCUS       XP_077505817             285 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115343 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505817
VERSION     XP_077505817.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649691.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..285
                     /product="uncharacterized protein LOC144115343 isoform X2"
                     /calculated_mol_wt=30505
     CDS             1..285
                     /gene="LOC144115343"
                     /coded_by="XM_077649691.1:7564..8421"
                     /db_xref="GeneID:144115343"
ORIGIN      
        1 mveplrcaga pmwrvlrgrr pptraqplde qrqllaqqap tkhrglalqw rhlspkeema
       61 veecyrwkpa yfsdetwckl pddikkcygn vhkdrsiker kgfgvkaqqq aviiqpvfvg
      121 aystaivhwq sgkaagshga taassggmra enrtlrhatf tfnlpeygag eeaetgvimv
      181 qrlllplrts epgllgggqh grghkavert aaiaalslla atyarhaggt dqqelrpsfa
      241 tpgaaaasyt gtafstgtlf cssansarar gndgsaavps atgtl
//
LOCUS       XP_077505818             285 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115343 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505818
VERSION     XP_077505818.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649692.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..285
                     /product="uncharacterized protein LOC144115343 isoform X2"
                     /calculated_mol_wt=30505
     CDS             1..285
                     /gene="LOC144115343"
                     /coded_by="XM_077649692.1:1086..1943"
                     /db_xref="GeneID:144115343"
ORIGIN      
        1 mveplrcaga pmwrvlrgrr pptraqplde qrqllaqqap tkhrglalqw rhlspkeema
       61 veecyrwkpa yfsdetwckl pddikkcygn vhkdrsiker kgfgvkaqqq aviiqpvfvg
      121 aystaivhwq sgkaagshga taassggmra enrtlrhatf tfnlpeygag eeaetgvimv
      181 qrlllplrts epgllgggqh grghkavert aaiaalslla atyarhaggt dqqelrpsfa
      241 tpgaaaasyt gtafstgtlf cssansarar gndgsaavps atgtl
//
LOCUS       XP_077505819             285 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115343 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505819
VERSION     XP_077505819.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649693.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..285
                     /product="uncharacterized protein LOC144115343 isoform X2"
                     /calculated_mol_wt=30505
     CDS             1..285
                     /gene="LOC144115343"
                     /coded_by="XM_077649693.1:705..1562"
                     /db_xref="GeneID:144115343"
ORIGIN      
        1 mveplrcaga pmwrvlrgrr pptraqplde qrqllaqqap tkhrglalqw rhlspkeema
       61 veecyrwkpa yfsdetwckl pddikkcygn vhkdrsiker kgfgvkaqqq aviiqpvfvg
      121 aystaivhwq sgkaagshga taassggmra enrtlrhatf tfnlpeygag eeaetgvimv
      181 qrlllplrts epgllgggqh grghkavert aaiaalslla atyarhaggt dqqelrpsfa
      241 tpgaaaasyt gtafstgtlf cssansarar gndgsaavps atgtl
//
LOCUS       XP_077505820             285 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115343 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505820
VERSION     XP_077505820.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649694.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..285
                     /product="uncharacterized protein LOC144115343 isoform X2"
                     /calculated_mol_wt=30505
     CDS             1..285
                     /gene="LOC144115343"
                     /coded_by="XM_077649694.1:909..1766"
                     /db_xref="GeneID:144115343"
ORIGIN      
        1 mveplrcaga pmwrvlrgrr pptraqplde qrqllaqqap tkhrglalqw rhlspkeema
       61 veecyrwkpa yfsdetwckl pddikkcygn vhkdrsiker kgfgvkaqqq aviiqpvfvg
      121 aystaivhwq sgkaagshga taassggmra enrtlrhatf tfnlpeygag eeaetgvimv
      181 qrlllplrts epgllgggqh grghkavert aaiaalslla atyarhaggt dqqelrpsfa
      241 tpgaaaasyt gtafstgtlf cssansarar gndgsaavps atgtl
//
LOCUS       XP_077505821             285 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115343 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505821
VERSION     XP_077505821.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649695.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..285
                     /product="uncharacterized protein LOC144115343 isoform X2"
                     /calculated_mol_wt=30505
     CDS             1..285
                     /gene="LOC144115343"
                     /coded_by="XM_077649695.1:836..1693"
                     /db_xref="GeneID:144115343"
ORIGIN      
        1 mveplrcaga pmwrvlrgrr pptraqplde qrqllaqqap tkhrglalqw rhlspkeema
       61 veecyrwkpa yfsdetwckl pddikkcygn vhkdrsiker kgfgvkaqqq aviiqpvfvg
      121 aystaivhwq sgkaagshga taassggmra enrtlrhatf tfnlpeygag eeaetgvimv
      181 qrlllplrts epgllgggqh grghkavert aaiaalslla atyarhaggt dqqelrpsfa
      241 tpgaaaasyt gtafstgtlf cssansarar gndgsaavps atgtl
//
LOCUS       XP_077505822             281 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115343 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505822
VERSION     XP_077505822.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649696.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..281
                     /product="uncharacterized protein LOC144115343 isoform X3"
                     /calculated_mol_wt=30039
     CDS             1..281
                     /gene="LOC144115343"
                     /coded_by="XM_077649696.1:7449..8294"
                     /db_xref="GeneID:144115343"
ORIGIN      
        1 mlscgapmwr vlrgrrpptr aqpldeqrql laqqaptkhr glalqwrhls pkeemaveec
       61 yrwkpayfsd etwcklpddi kkcygnvhkd rsikerkgfg vkaqqqavii qpvfvgayst
      121 aivhwqsgka agshgataas sggmraenrt lrhatftfnl peygageeae tgvimvqrll
      181 lplrtsepgl lgggqhgrgh kavertaaia alsllaatya rhaggtdqqe lrpsfatpga
      241 aaasytgtaf stgtlfcssa nsarargndg saavpsatgt l
//
LOCUS       XP_077505823             274 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115343 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505823
VERSION     XP_077505823.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649697.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..274
                     /product="uncharacterized protein LOC144115343 isoform X4"
                     /calculated_mol_wt=29379
     CDS             1..274
                     /gene="LOC144115343"
                     /coded_by="XM_077649697.1:258..1082"
                     /db_xref="GeneID:144115343"
ORIGIN      
        1 mwrvlrgrrp ptraqpldeq rqllaqqapt khrglalqwr hlspkeemav eecyrwkpay
       61 fsdetwcklp ddikkcygnv hkdrsikerk gfgvkaqqqa viiqpvfvga ystaivhwqs
      121 gkaagshgat aassggmrae nrtlrhatft fnlpeygage eaetgvimvq rlllplrtse
      181 pgllgggqhg rghkaverta aiaalsllaa tyarhaggtd qqelrpsfat pgaaaasytg
      241 tafstgtlfc ssansararg ndgsaavpsa tgtl
//
LOCUS       XP_077505824             362 aa            linear   INV 25-NOV-2025
DEFINITION  tachykinin-like peptides receptor 99D [Amblyomma americanum].
ACCESSION   XP_077505824
VERSION     XP_077505824.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649698.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..362
                     /product="tachykinin-like peptides receptor 99D"
                     /calculated_mol_wt=40679
     Region          37..326
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          39..63
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320515"
     Region          72..94
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320515"
     Region          110..132
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320515"
     Region          154..170
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320515"
     Region          203..226
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320515"
     Region          257..279
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320515"
     Region          294..319
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320515"
     CDS             1..362
                     /gene="LOC144115347"
                     /coded_by="XM_077649698.1:60..1148"
                     /db_xref="GeneID:144115347"
ORIGIN      
        1 mdpannqsla ngslpappln assdddelyq vpveiivlls fcygiislva vagnsmvlwi
       61 vatsrrmqtv tnffianlav adiiiglfsi pfqfqaallq rwvlpefmca fcpfvqvlsv
      121 nvsiftltai aldryravta plrarccskf nakvligvvw avstaaalpy alalrvilvy
      181 dsvsgevsrp fcinvvlppf awkvynhvlv glqyflplcv icytygrigg avrnsrtpgn
      241 aetvrdtniv rnkrkvtkmi lvvvaifaic wlpyqtynil qelypiinqy ryinviwfcs
      301 hwlamsnscy npfiyaiyse kfsaefrarm rccssrskqd eahcyrqtpn svstrysmrs
      361 ys
//
LOCUS       XP_077505825            1722 aa            linear   INV 25-NOV-2025
DEFINITION  methylcytosine dioxygenase TET-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505825
VERSION     XP_077505825.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649699.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1722
                     /product="methylcytosine dioxygenase TET-like isoform X1"
                     /calculated_mol_wt=187020
     Region          <200..494
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          646..>960
                     /region_name="Tet_JBP"
                     /note="oxygenase domain of ten-eleven translocation (TET)
                     enzymes, J-binding proteins (JBPs), and similar proteins;
                     cl40427"
                     /db_xref="CDD:394797"
     Region          <1582..1658
                     /region_name="Tet_JBP"
                     /note="oxygenase domain of ten-eleven translocation (TET)
                     enzymes, J-binding proteins (JBPs), and similar proteins;
                     cl40427"
                     /db_xref="CDD:394797"
     CDS             1..1722
                     /gene="LOC144115350"
                     /coded_by="XM_077649699.1:193..5361"
                     /db_xref="GeneID:144115350"
ORIGIN      
        1 mmhqmherrr gtpdenknlq qvasaicgsg vagkdgmass pggrnnlngr tlngsttpyl
       61 pqgvpehlgh pgiggfsghs eyqlpptaks gtaggpnqmy esgpsfgtfp aqhpqavgaf
      121 nghvqqqqqq qqqqqqqqqq qqqqqqqqqq qqqqqqqqqq qqqqqqqqqh dgrlplgtee
      181 pdfptrhqmm nnrlktliqt rqsqkeqgpf qglestlgpa gnfvhpqpaq pvpdeqpmgh
      241 qqfsrqtaev gpprnfppla rsnsgssnsg shsngnarpp ssselpaeaw heanpsvgqa
      301 rgrpelpefa kngsssqgsn nseaefsqpe dqsresefad ssaepgnapr apsgpavptn
      361 gmlpygsfyg apqepteaka dacenkktps tsappasead irggapyhyt saasigtttt
      421 asavashpkq pcngpappsp fagrqspraa fqgappyvph lvppspvrgn esdpshqqpr
      481 seseptdavq qpflsnttts mstytsiist ynsfatscap sylpeqpphh hhqqltaagg
      541 skfggdgaqd gaaraggapd idagriaavg efrglelglg plggppppfg gfaegplaya
      601 gppfplpsmg ppgtphfapl tlpgadpwwe rlerlrsnak aeppacdcls pedappldks
      661 pyythlgsgp tvaavremle rrlnvtgtal riekvlytgk egktsqgcpv akwvirrsgp
      721 sekvlavlrh rqghrcmcay ivmaivaweg vhadmaddly rtvvhktvnf gfptqrrcgt
      781 neqrtcacqg idsetcgasf sfgcswsmyy ngckyarska vrkfklseqs eeqeledklq
      841 qlatdmaply arvapesykn qtefesegis crlglkpgrp fsgvtacvdy cahahkdlhn
      901 mnngctvvvt ltkhrgfekg ddeqlhvlpl yvldatdeyg kkdgffekvk agslevltky
      961 htttrirkvp lgpckkrgrk eknpdeaaht iqqnsnleaq tdegyasqtm pspvfspsfe
     1021 apppsyhrsp feekyksgvp hvkheselgs prfeipaspf rsqqqpsfhp tsepsaakpy
     1081 gtcapaykvk necvpplscq ydasapessf slqkpsspca esqvpcpgkd dmkvvikken
     1141 pseaciqsil dkgaqvlnrs sgmpqslsph tlerahhtkd snpfnqpylq pqqdlaygnp
     1201 yfagnyaqqg aqkmaslctl pqspmmttip dtppedhhla mnyykyrmvd ryhypsylph
     1261 sgqdgpsveg hsidhlqnla svsnhhlqnl elgrpsggha vyspfvdrsa smqsyhhqsl
     1321 satfnpisqt psqgvmlpps yvpqavsphm arsagygapg fsrsgsflss pheafpphps
     1381 ppvtpmtpps tprltelnpv rmdsplhmrs pyymagsfgm pvdpscgasr shsqgapfpq
     1441 apyvdaaawa sdrdysagfq gpyyaprgqc fpaavtppkd ygmhtppvag ptrlpaglat
     1501 pppqtrclqe rasvlppfkn tflpaqtapn gferhlqtfr svmedaqrsq vagparlmpr
     1561 pasheglpra eitkadeegi yevdsdneaa fqdpevggva ialthgsvlf ecakhelhat
     1621 talrrpdrkn ptrislvfyq hknlnfrnhg eeewekkmev rklekanger pskkkaklst
     1681 erldedgfpa salrsadqap swegphvplp sffainpyqk cf
//
LOCUS       XP_077505826            1671 aa            linear   INV 25-NOV-2025
DEFINITION  methylcytosine dioxygenase TET-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505826
VERSION     XP_077505826.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649700.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1671
                     /product="methylcytosine dioxygenase TET-like isoform X2"
                     /calculated_mol_wt=182079
     Region          <149..443
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          595..>909
                     /region_name="Tet_JBP"
                     /note="oxygenase domain of ten-eleven translocation (TET)
                     enzymes, J-binding proteins (JBPs), and similar proteins;
                     cl40427"
                     /db_xref="CDD:394797"
     Region          <1531..1607
                     /region_name="Tet_JBP"
                     /note="oxygenase domain of ten-eleven translocation (TET)
                     enzymes, J-binding proteins (JBPs), and similar proteins;
                     cl40427"
                     /db_xref="CDD:394797"
     CDS             1..1671
                     /gene="LOC144115350"
                     /coded_by="XM_077649700.1:193..5208"
                     /db_xref="GeneID:144115350"
ORIGIN      
        1 mmhqmherrr gtpdenknlq qiggfsghse yqlpptaksg taggpnqmye sgpsfgtfpa
       61 qhpqavgafn ghvqqqqqqq qqqqqqqqqq qqqqqqqqqq qqqqqqqqqq qqqqqqqqhd
      121 grlplgteep dfptrhqmmn nrlktliqtr qsqkeqgpfq glestlgpag nfvhpqpaqp
      181 vpdeqpmghq qfsrqtaevg pprnfpplar snsgssnsgs hsngnarpps sselpaeawh
      241 eanpsvgqar grpelpefak ngsssqgsnn seaefsqped qsresefads saepgnapra
      301 psgpavptng mlpygsfyga pqepteakad acenkktpst sappaseadi rggapyhyts
      361 aasigtttta savashpkqp cngpappspf agrqspraaf qgappyvphl vppspvrgne
      421 sdpshqqprs eseptdavqq pflsntttsm stytsiisty nsfatscaps ylpeqpphhh
      481 hqqltaaggs kfggdgaqdg aaraggapdi dagriaavge frglelglgp lggppppfgg
      541 faegplayag ppfplpsmgp pgtphfaplt lpgadpwwer lerlrsnaka eppacdclsp
      601 edappldksp yythlgsgpt vaavremler rlnvtgtalr iekvlytgke gktsqgcpva
      661 kwvirrsgps ekvlavlrhr qghrcmcayi vmaivawegv hadmaddlyr tvvhktvnfg
      721 fptqrrcgtn eqrtcacqgi dsetcgasfs fgcswsmyyn gckyarskav rkfklseqse
      781 eqeledklqq latdmaplya rvapesyknq tefesegisc rlglkpgrpf sgvtacvdyc
      841 ahahkdlhnm nngctvvvtl tkhrgfekgd deqlhvlply vldatdeygk kdgffekvka
      901 gslevltkyh tttrirkvpl gpckkrgrke knpdeaahti qqnsnleaqt degyasqtmp
      961 spvfspsfea pppsyhrspf eekyksgvph vkheselgsp rfeipaspfr sqqqpsfhpt
     1021 sepsaakpyg tcapaykvkn ecvpplscqy dasapessfs lqkpsspcae sqvpcpgkdd
     1081 mkvvikkenp seaciqsild kgaqvlnrss gmpqslspht lerahhtkds npfnqpylqp
     1141 qqdlaygnpy fagnyaqqga qkmaslctlp qspmmttipd tppedhhlam nyykyrmvdr
     1201 yhypsylphs gqdgpsvegh sidhlqnlas vsnhhlqnle lgrpsgghav yspfvdrsas
     1261 mqsyhhqsls atfnpisqtp sqgvmlppsy vpqavsphma rsagygapgf srsgsflssp
     1321 heafpphpsp pvtpmtppst prltelnpvr mdsplhmrsp yymagsfgmp vdpscgasrs
     1381 hsqgapfpqa pyvdaaawas drdysagfqg pyyaprgqcf paavtppkdy gmhtppvagp
     1441 trlpaglatp ppqtrclqer asvlppfknt flpaqtapng ferhlqtfrs vmedaqrsqv
     1501 agparlmprp asheglprae itkadeegiy evdsdneaaf qdpevggvai althgsvlfe
     1561 cakhelhatt alrrpdrknp trislvfyqh knlnfrnhge eewekkmevr klekangerp
     1621 skkkaklste rldedgfpas alrsadqaps wegphvplps ffainpyqkc f
//
LOCUS       XP_077505827             495 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115351 [Amblyomma americanum].
ACCESSION   XP_077505827
VERSION     XP_077505827.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649701.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..495
                     /product="uncharacterized protein LOC144115351"
                     /calculated_mol_wt=54448
     Region          29..76
                     /region_name="zf-C3HC4_3"
                     /note="Zinc finger, C3HC4 type (RING finger); pfam13920"
                     /db_xref="CDD:464042"
     Region          <180..>391
                     /region_name="COG4913"
                     /note="Uncharacterized conserved protein, contains a
                     C-terminal ATPase domain [Function unknown]"
                     /db_xref="CDD:443941"
     CDS             1..495
                     /gene="LOC144115351"
                     /coded_by="XM_077649701.1:75..1562"
                     /db_xref="GeneID:144115351"
ORIGIN      
        1 mapasaqtvf cfgtgldwpp tcfvnpfpss rvcsvcclvp ssaamlacrh llcqscydgv
       61 dakrshcpld kepfqeddva wttigrdgtl grkvrcwnae hgcdnegvas amlehfinac
      121 qfhaesctrc seevlhrdia ehlecdcvps rpqeqplddn ianafmevke algkilegna
      181 avrrkldsle drlrpeagst fatqststad tatvalensi pvlraqaeat fpeyravqaa
      241 lnklseenav lhtkldllee hldmenamat lstmvsdals tavlktaavc raeteaaldk
      301 rwgqvpteis qamagnvrav kgvisrecqr nvlglkasiv eafrgdrrla dagatsseta
      361 eklteivdde vkamellvva slsstndfln ksvlyeltve dwtefctkap lsaqyftgnd
      421 grpsyhygyl ivprlclsds kvwlrvymie glfdrflkwp mekkikllfi npcdstrela
      481 iesaisqelt rplae
//
LOCUS       XP_077505828             443 aa            linear   INV 25-NOV-2025
DEFINITION  ras association family member [Amblyomma americanum].
ACCESSION   XP_077505828
VERSION     XP_077505828.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649702.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..443
                     /product="ras association family member"
                     /calculated_mol_wt=50356
     Region          4..57
                     /region_name="LIM_TLP_like"
                     /note="The LIM domains of thymus LIM protein (TLP);
                     cd09401"
                     /db_xref="CDD:188785"
     Site            order(4,7,25,28,31,34,52,56)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188785"
     Region          292..378
                     /region_name="RA_RASSF2_like"
                     /note="Ras-associating (RA) domain found in
                     Ras-association domain family members, RASSF2, RASSF4, and
                     RASSF6; cd01784"
                     /db_xref="CDD:340482"
     Region          389..433
                     /region_name="SARAH_RASSF2-like"
                     /note="C-terminal SARAH domain found in Ras-association
                     domain proteins, RASSF2, RASSF4, and RASSF6; cd21886"
                     /db_xref="CDD:439180"
     Site            order(390..391,393,395,398,402,405..406,408..409,412..413,
                     416..417,419..420,423..424,427,430..431)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:439180"
     CDS             1..443
                     /gene="Rassf"
                     /coded_by="XM_077649702.1:321..1652"
                     /db_xref="GeneID:144115352"
ORIGIN      
        1 mwtcrkcgkp vyfaerktsm gyewhpeclr ceecgkrlnp gqhaehkgvp ychvpcysvl
       61 fgptlfghgs qveahtsfgk ntkdrqciss vsrnhlegkl rvynqfyegk sgivqsrlrn
      121 grlilegsir iywgvnrvir lkedhddrip vrrrrtslrl srlainsknr gyvqngspek
      181 eastglenga gksgapktss snsdlfvetq lsdpessesq vngspseigr syrtlpnpgk
      241 hslsdllads svqptpaced angfegvtlr skarssrvkl rrrcsinghf ynretsvftp
      301 aygsvtsvwv tslvnapevi nqlldkfkve nsprdfalyv vrdtgerrlm qddeypllar
      361 vmlgpdetva klfimnrent neipyevaqy lnfseveltt fikkfheeed revekiknkf
      421 kilrhriqtr mqelksipvk fnt
//
LOCUS       XP_077505830             553 aa            linear   INV 25-NOV-2025
DEFINITION  arylsulfatase B-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505830
VERSION     XP_077505830.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649704.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..553
                     /product="arylsulfatase B-like isoform X1"
                     /calculated_mol_wt=60780
     Region          35..413
                     /region_name="4-S"
                     /note="N-acetylgalactosamine 4-sulfatase, also called
                     arylsulftase B; cd16029"
                     /db_xref="CDD:293753"
     Site            order(43..44,82,136,138,230,293..294)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:293753"
     Site            order(43..44,82,136,138,230,294)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:293753"
     Region          <487..517
                     /region_name="DUF4976"
                     /note="Domain of unknown function (DUF4976); cl20644"
                     /db_xref="CDD:419068"
     CDS             1..553
                     /gene="LOC144115353"
                     /coded_by="XM_077649704.1:121..1782"
                     /db_xref="GeneID:144115353"
ORIGIN      
        1 mesarvifll vclallfgta wlllrqdaaq laprphivfv laddlgwndv syhgcpqirt
       61 pnidalawng irlrryyaqp lctpsraall sgryplnlgl qhsviyneep rglplsvsll
      121 pqwlaklgya thilgkwhvg fckeeytptm rgfqrhvgfw gayidyykhe kdylgptrsp
      181 gldmrrdflp apndtgryit hllteeavav ieghpddkpl flylahlaph saslrdplqv
      241 pdkyvepyrd igspermkya gmvstldesv glvfealakr gilnntlfvf csdngadvag
      301 gnanhaspwp lrgqkyspwe ggvraaalvw spalshskga dyeqlfhitd wlptlyqlag
      361 gvpaelgdid gvshadslaa nstspprtel vvnidpvdny saiieghfkl vagvaeggkf
      421 ehwypivgnv nwsstepleq ceassvarvl tslgldpacg lgggtyptpi dcgsrdpekt
      481 crptvapclf dlakdpceyn dvapehpelv ksllaklgry kaaatppanl phdplsdpgl
      541 ynnvwipwre klp
//
LOCUS       XP_077505831             514 aa            linear   INV 25-NOV-2025
DEFINITION  arylsulfatase B-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505831
VERSION     XP_077505831.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649705.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..514
                     /product="arylsulfatase B-like isoform X2"
                     /calculated_mol_wt=55982
     Region          35..374
                     /region_name="4-S"
                     /note="N-acetylgalactosamine 4-sulfatase, also called
                     arylsulftase B; cd16029"
                     /db_xref="CDD:293753"
     Site            order(43..44,82,136,191,254..255)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:293753"
     Site            order(43..44,82,136,191,255)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:293753"
     Region          <448..478
                     /region_name="DUF4976"
                     /note="Domain of unknown function (DUF4976); cl20644"
                     /db_xref="CDD:419068"
     CDS             1..514
                     /gene="LOC144115353"
                     /coded_by="XM_077649705.1:122..1666"
                     /db_xref="GeneID:144115353"
ORIGIN      
        1 mesarvifll vclallfgta wlllrqdaaq laprphivfv laddlgwndv syhgcpqirt
       61 pnidalawng irlrryyaqp lctpsraall sgryplnlgl qhsviyneep rglplsvsll
      121 pqwlaklgya thilgkptrs pgldmrrdfl papndtgryi thllteeava vieghpddkp
      181 lflylahlap hsaslrdplq vpdkyvepyr digspermky agmvstldes vglvfealak
      241 rgilnntlfv fcsdngadva ggnanhaspw plrgqkyspw eggvraaalv wspalshskg
      301 adyeqlfhit dwlptlyqla ggvpaelgdi dgvshadsla anstspprte lvvnidpvdn
      361 ysaiieghfk lvagvaeggk fehwypivgn vnwssteple qceassvarv ltslgldpac
      421 glgggtyptp idcgsrdpek tcrptvapcl fdlakdpcey ndvapehpel vksllaklgr
      481 ykaaatppan lphdplsdpg lynnvwipwr eklp
//
LOCUS       XP_077505832             454 aa            linear   INV 25-NOV-2025
DEFINITION  arylsulfatase B-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077505832
VERSION     XP_077505832.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649706.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..454
                     /product="arylsulfatase B-like isoform X3"
                     /calculated_mol_wt=50123
     Region          35..314
                     /region_name="4-S"
                     /note="N-acetylgalactosamine 4-sulfatase, also called
                     arylsulftase B; cd16029"
                     /db_xref="CDD:293753"
     Site            order(43..44,82,136,138,230)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:293753"
     Region          <388..418
                     /region_name="DUF4976"
                     /note="Domain of unknown function (DUF4976); cl20644"
                     /db_xref="CDD:419068"
     CDS             1..454
                     /gene="LOC144115353"
                     /coded_by="XM_077649706.1:122..1486"
                     /db_xref="GeneID:144115353"
ORIGIN      
        1 mesarvifll vclallfgta wlllrqdaaq laprphivfv laddlgwndv syhgcpqirt
       61 pnidalawng irlrryyaqp lctpsraall sgryplnlgl qhsviyneep rglplsvsll
      121 pqwlaklgya thilgkwhvg fckeeytptm rgfqrhvgfw gayidyykhe kdylgptrsp
      181 gldmrrdflp apndtgryit hllteeavav ieghpddkpl flylahlaph saslrdplqv
      241 pdkyvepyrd igspermkya ggvpaelgdi dgvshadsla anstspprte lvvnidpvdn
      301 ysaiieghfk lvagvaeggk fehwypivgn vnwssteple qceassvarv ltslgldpac
      361 glgggtyptp idcgsrdpek tcrptvapcl fdlakdpcey ndvapehpel vksllaklgr
      421 ykaaatppan lphdplsdpg lynnvwipwr eklp
//
LOCUS       XP_077505833             468 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115354 [Amblyomma americanum].
ACCESSION   XP_077505833
VERSION     XP_077505833.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649707.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..468
                     /product="uncharacterized protein LOC144115354"
                     /calculated_mol_wt=52097
     Region          33..>69
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     CDS             1..468
                     /gene="LOC144115354"
                     /coded_by="XM_077649707.1:319..1725"
                     /db_xref="GeneID:144115354"
ORIGIN      
        1 mapaslqtvf gidsgfdwrp tcfvnpfppt rvcsvcglvp ssaamlpclh llcqscsegd
       61 gakrnhcpfh kepfqeedvv wstfsrdsil grkvrcwnae hgcdaedvas amlehfanac
      121 qfhvvrcpec sesvrhrdis ehlacdcepq rahyqasddn fsnafmevkg tlakileens
      181 alhtkmdsfe drlrtdtsga faaqstsiad avtatlerks selttgaeaa laedqrevta
      241 evrdalayvq rtmkenskac ewnvscledr vvkavceqrk padagsslse mtaakkeaas
      301 vedeakalel lavaslsirs dvlsksvpye wtiddwngfc rkssdrlhyf tgndgrpsyh
      361 ygyllvsrlc fsrvyvwfrv yiieglfdkf lkwpiekkvk lllinpcdct rqlalsssls
      421 plpkhplpgf skevefvgss htnitvdafn krglignnkl qlrlefes
//
LOCUS       XP_077505834             170 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-4-like [Amblyomma americanum].
ACCESSION   XP_077505834
VERSION     XP_077505834.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649708.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..170
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..170
                     /product="protein argonaute-4-like"
                     /calculated_mol_wt=18828
     Region          <7..126
                     /region_name="Piwi-like"
                     /note="PIWI domain. Domain found in proteins involved in
                     RNA silencing. RNA silencing refers to a group of related
                     gene-silencing mechanisms mediated by short RNA molecules,
                     including siRNAs, miRNAs, and heterochromatin-related
                     guide RNAs. The central component...; cl00628"
                     /db_xref="CDD:412485"
     CDS             1..170
                     /gene="LOC144115355"
                     /coded_by="XM_077649708.1:16..528"
                     /db_xref="GeneID:144115355"
ORIGIN      
        1 mcptetyepp ltfivvqkrh ltrfmpandr dgvgkfrnvp pgttvdsvvt hpldfdfflc
       61 shfgiqgtsr pahyyivwdd snfsaddlqk lsfylchtys rcarsvsipa pvyyahlaaf
      121 rakhhiaskl etsgavsqss egggdavltt aqyveavkvl eelqasmyfv
//
LOCUS       XP_077505835             603 aa            linear   INV 25-NOV-2025
DEFINITION  carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase
            protein-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505835
VERSION     XP_077505835.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649709.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..603
                     /product="carboxyl-terminal PDZ ligand of neuronal nitric
                     oxide synthase protein-like isoform X1"
                     /calculated_mol_wt=64836
     Region          3..180
                     /region_name="PTB_CAPON-like"
                     /note="Carboxyl-terminal PDZ ligand of neuronal nitric
                     oxide synthase protein (CAPON) Phosphotyrosine-binding
                     (PTB) domain; cd01270"
                     /db_xref="CDD:269968"
     Site            order(36,122,142)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:269968"
     Site            order(111..116,129,153,157)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:269968"
     Region          <311..>404
                     /region_name="MukB"
                     /note="Chromosome condensin MukBEF, ATPase and DNA-binding
                     subunit MukB [Cell cycle control, cell division,
                     chromosome partitioning]; COG3096"
                     /db_xref="CDD:442330"
     CDS             1..603
                     /gene="LOC144115356"
                     /coded_by="XM_077649709.1:534..2345"
                     /db_xref="GeneID:144115356"
ORIGIN      
        1 mpskkqynlv sddaydsrip lhseeafqhg isfnakyigt mdvprpssrv eivaamrrir
       61 yefkaksikk kkvtitisvd gvkiilrkkh knqwawddnr ylimnhpiyr ifyvshdsqd
      121 lkifsyiard ggsngfkcnv fkskkksqam rvvrtigqaf evchklslan atsnlvttds
      181 lpsgarhsss dnsisrdkdq sekfdrdstc gdtkplapqs gaspletphq dshcsskass
      241 sspatgssqq tllqpqqsqs qqqqvsqslk paanqvvctq pvvsattega slstpppamp
      301 vslvpegtsl slyhqlqlmr aqmehqaqqa qsaisqarhl rdqlsaeata rseamsqnqq
      361 livhnrellq hihllvkhiq elekkinrle gksdhlqpdl spsplqigpp prfldllsga
      421 qpvgktpspv ppvgepfelg slawmsgsvg vggspppatp phqqptaast ansspsitts
      481 slqlrlddls fsgamkgttn edmrgggrsp aarvatvpgs lpelpvfadg gsstssaspp
      541 pwssgpvppl sissaglfqr qfsaplpssq tslsrlpdis rltidegeps lhkflgagas
      601 gks
//
LOCUS       XP_077505836             561 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115356 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505836
VERSION     XP_077505836.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649710.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..561
                     /product="uncharacterized protein LOC144115356 isoform X2"
                     /calculated_mol_wt=59343
     Region          <105..138
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          <269..>362
                     /region_name="MukB"
                     /note="Chromosome condensin MukBEF, ATPase and DNA-binding
                     subunit MukB [Cell cycle control, cell division,
                     chromosome partitioning]; COG3096"
                     /db_xref="CDD:442330"
     CDS             1..561
                     /gene="LOC144115356"
                     /coded_by="XM_077649710.1:115..1800"
                     /db_xref="GeneID:144115356"
ORIGIN      
        1 mrrafsqkfg rsgsrteqgk asqekesalq tnkvvkkisf repevdneyk aslscistka
       61 aqnatrdskt tnsistakrc tprkdlartv glqrsisled idlqsqamrv vrtigqafev
      121 chklslanat snlvttdslp sgarhsssdn sisrdkdqse kfdrdstcgd tkplapqsga
      181 spletphqds hcsskassss patgssqqtl lqpqqsqsqq qqvsqslkpa anqvvctqpv
      241 vsattegasl stpppampvs lvpegtslsl yhqlqlmraq mehqaqqaqs aisqarhlrd
      301 qlsaeatars eamsqnqqli vhnrellqhi hllvkhiqel ekkinrlegk sdhlqpdlsp
      361 splqigpppr fldllsgaqp vgktpspvpp vgepfelgsl awmsgsvgvg gspppatpph
      421 qqptaastan sspsittssl qlrlddlsfs gamkgttned mrgggrspaa rvatvpgslp
      481 elpvfadggs stssaspppw ssgpvpplsi ssaglfqrqf saplpssqts lsrlpdisrl
      541 tidegepslh kflgagasgk s
//
LOCUS       XP_077505837             621 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase COP1-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505837
VERSION     XP_077505837.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649711.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..621
                     /product="E3 ubiquitin-protein ligase COP1-like isoform
                     X1"
                     /calculated_mol_wt=70716
     Region          38..84
                     /region_name="RING-HC_COP1"
                     /note="RING finger, HC subclass, found in constitutive
                     photomorphogenesis protein 1 (COP1) and similar proteins;
                     cd16504"
                     /db_xref="CDD:438167"
     Region          <144..621
                     /region_name="PLN00181"
                     /note="protein SPA1-RELATED; Provisional"
                     /db_xref="CDD:177776"
     Region          319..353
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          358..404
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          411..447
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          452..490
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          496..535
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          539..561
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          594..620
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..621
                     /gene="LOC144115357"
                     /coded_by="XM_077649711.1:241..2106"
                     /db_xref="GeneID:144115357"
ORIGIN      
        1 masanntris iadhnrrark rqpsvlngis ssyedrnsdf lcpicfdiie eahmtpcght
       61 fcfkcittgl eynnrcpkcn fvvekkeqiy pnfllnelit kykqraadkk mklqgnnqvv
      121 selhelivqe sdnmslndvy nmldvlsekk qqleadckaa qaqllkefle qvrkhkqeqm
      181 dqlttelsfi dedlksveen akdpehfwlv dsgshlpeea akpenptsmq dgfngskygs
      241 kpqwlqstla srrkrvhlhf ddleecyltt rtkhvnynsa dglkeftedl skftryssir
      301 platlnyatd llngtsivss iefdkdneff aiagvtkkik vfeygtviqd ivdihypvne
      361 mmcnskisci swssyhkgml assdyegtvt iwdaftgqkv kmfqehekrc wsvdfnkvdt
      421 kiiasgsdda kvklwsiacd hsvisleaka nvccvkfnpa srfhlalgsa dhcvyyydlr
      481 stkeplcvfk ghkkavsyvk flntnelvsa stdsqlklwd iyfpycqrsf kghlneknfv
      541 glatdgdyva cgsennalyi yykglskqvl kfrfdvvrni lekdkreeds sefvsavcwr
      601 mgssvvvaan sqgtikvlel v
//
LOCUS       XP_077505838             616 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase COP1-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505838
VERSION     XP_077505838.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649712.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..616
                     /product="E3 ubiquitin-protein ligase COP1-like isoform
                     X2"
                     /calculated_mol_wt=70120
     Region          38..84
                     /region_name="RING-HC_COP1"
                     /note="RING finger, HC subclass, found in constitutive
                     photomorphogenesis protein 1 (COP1) and similar proteins;
                     cd16504"
                     /db_xref="CDD:438167"
     Region          <144..616
                     /region_name="PLN00181"
                     /note="protein SPA1-RELATED; Provisional"
                     /db_xref="CDD:177776"
     Region          319..353
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          358..404
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          411..447
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          452..490
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          496..535
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          539..561
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          589..615
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..616
                     /gene="LOC144115357"
                     /coded_by="XM_077649712.1:241..2091"
                     /db_xref="GeneID:144115357"
ORIGIN      
        1 masanntris iadhnrrark rqpsvlngis ssyedrnsdf lcpicfdiie eahmtpcght
       61 fcfkcittgl eynnrcpkcn fvvekkeqiy pnfllnelit kykqraadkk mklqgnnqvv
      121 selhelivqe sdnmslndvy nmldvlsekk qqleadckaa qaqllkefle qvrkhkqeqm
      181 dqlttelsfi dedlksveen akdpehfwlv dsgshlpeea akpenptsmq dgfngskygs
      241 kpqwlqstla srrkrvhlhf ddleecyltt rtkhvnynsa dglkeftedl skftryssir
      301 platlnyatd llngtsivss iefdkdneff aiagvtkkik vfeygtviqd ivdihypvne
      361 mmcnskisci swssyhkgml assdyegtvt iwdaftgqkv kmfqehekrc wsvdfnkvdt
      421 kiiasgsdda kvklwsiacd hsvisleaka nvccvkfnpa srfhlalgsa dhcvyyydlr
      481 stkeplcvfk ghkkavsyvk flntnelvsa stdsqlklwd iyfpycqrsf kghlneknfv
      541 glatdgdyva cgsennalyi yykglskqvl kfrfdvekdk reedssefvs avcwrmgssv
      601 vvaansqgti kvlelv
//
LOCUS       XP_077505839             586 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase COP1-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505839
VERSION     XP_077505839.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649713.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..586
                     /product="E3 ubiquitin-protein ligase COP1-like isoform
                     X3"
                     /calculated_mol_wt=66687
     Region          8..49
                     /region_name="RING-HC_COP1"
                     /note="RING finger, HC subclass, found in constitutive
                     photomorphogenesis protein 1 (COP1) and similar proteins;
                     cd16504"
                     /db_xref="CDD:438167"
     Region          <109..586
                     /region_name="PLN00181"
                     /note="protein SPA1-RELATED; Provisional"
                     /db_xref="CDD:177776"
     Region          284..318
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          323..369
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          376..412
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          417..455
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          461..500
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          504..526
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          559..585
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..586
                     /gene="LOC144115357"
                     /coded_by="XM_077649713.1:74..1834"
                     /db_xref="GeneID:144115357"
ORIGIN      
        1 mfsvspgpic fdiieeahmt pcghtfcfkc ittgleynnr cpkcnfvvek keqiypnfll
       61 nelitkykqr aadkkmklqg nnqvvselhe livqesdnms lndvynmldv lsekkqqlea
      121 dckaaqaqll kefleqvrkh kqeqmdqltt elsfidedlk sveenakdpe hfwlvdsgsh
      181 lpeeaakpen ptsmqdgfng skygskpqwl qstlasrrkr vhlhfddlee cylttrtkhv
      241 nynsadglke ftedlskftr yssirplatl nyatdllngt sivssiefdk dneffaiagv
      301 tkkikvfeyg tviqdivdih ypvnemmcns kisciswssy hkgmlassdy egtvtiwdaf
      361 tgqkvkmfqe hekrcwsvdf nkvdtkiias gsddakvklw siacdhsvis leakanvccv
      421 kfnpasrfhl algsadhcvy yydlrstkep lcvfkghkka vsyvkflntn elvsastdsq
      481 lklwdiyfpy cqrsfkghln eknfvglatd gdyvacgsen nalyiyykgl skqvlkfrfd
      541 vvrnilekdk reedssefvs avcwrmgssv vvaansqgti kvlelv
//
LOCUS       XP_077505840             710 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC144115358
            [Amblyomma americanum].
ACCESSION   XP_077505840
VERSION     XP_077505840.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649714.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 2 indels
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..710
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC144115358"
                     /calculated_mol_wt=77789
     Region          199..>379
                     /region_name="THH1_TOM1-3_dom"
                     /note="THH1/TOM1/TOM3 domain; pfam06454"
                     /db_xref="CDD:399452"
     Region          515..567
                     /region_name="RING_CH-C4HC3_MARCH"
                     /note="RING-CH finger, H2 subclass (C4HC3-type), found in
                     membrane-associated RING-CH proteins (MARCH); cd16495"
                     /db_xref="CDD:438158"
     CDS             1..710
                     /gene="LOC144115358"
                     /coded_by="XM_077649714.1:16..2148"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 2 bases in 2 codons"
                     /db_xref="GeneID:144115358"
ORIGIN      
        1 msscasslaq qsgrfllncm kfaeietdaq hylagqyfll svafpiaack fsvrrdktar
       61 asalvrskga drskcarasv fsslipgcds scghlqlkcc gfrpsltaag trvvapqhva
      121 gvawlcfvsm vdcreppptt svthlpskly pllknfmtvh pnasctdncs ghgechqgic
      181 fcevqysggt ctdpnlsyfv afstifylic avsfvqllvc irsefsrikp psllkafkvt
      241 tqkalyalic iatfargfyf sspenaslew asslmsayyp llltgssliv cfwaevfhlr
      301 dlrhdkprfl sksflgflaf niitysllla elvltqfagy geterslflg vfngsfavlm
      361 vivvvffliy gvevyfkvrg afvagpptlp nmsqvhqsrl glvafatlll tmvlfifsdv
      421 mgafwkdkva vlgrnchdvm frviemglal wfpcvlwncv qpqqlwilnp kkllvrrepe
      481 kvaptgtese alfsvpkela ksgdslssle klpecficyd tertdagpli rpcnckgdva
      541 vvhhdclrtw liesagnsds nrckvcneey elergevwlp sglstahwlq taavlvimgs
      601 avsgaclvvk vfdnvgirtl avglallvey vclrllgisv leayqrakys avrilgrrls
      661 gpsaerivta tpsarlvvpr tgghlpadhr amlttalvtp qpaaeselvi
//
LOCUS       XP_077505841             317 aa            linear   INV 25-NOV-2025
DEFINITION  aldo-keto reductase 1B-like [Amblyomma americanum].
ACCESSION   XP_077505841
VERSION     XP_077505841.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649715.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..317
                     /product="aldo-keto reductase 1B-like"
                     /calculated_mol_wt=35875
     Region          10..299
                     /region_name="AKR_SF"
                     /note="Aldo-keto reductase (AKR) superfamily; cl00470"
                     /db_xref="CDD:444925"
     Site            order(22..24,47,52,81,114,163..164,185,211..217,247,
                     262..265,270,273..274)
                     /site_type="active"
                     /db_xref="CDD:381296"
     Site            order(47,52,81,114)
                     /site_type="active"
                     /note="catalytic tetrad [active]"
                     /db_xref="CDD:381296"
     CDS             1..317
                     /gene="LOC144115359"
                     /coded_by="XM_077649715.1:180..1133"
                     /db_xref="GeneID:144115359"
ORIGIN      
        1 mtskvpfvkl ssgykiptig lgtwksqvgq vyeavkiaid agyrhidcaf ayqneeevgr
       61 aiadkikdgt verkdlwitg kcwntyhsrs kvfeccelsl kklcldyfdl ylmhwpmgyq
      121 eggemfpkne agdfifsdvd yletwaamee cvekglvrsi gvcnfnkdql lrllgaakik
      181 pamlqvechp ylnqsdlinf ckehdvavta ysplgspdrp wakagdpllm eepaikaiae
      241 ahgktaaqvl iryqiergvi vipksvtker iisnldvfdf kltpkemeti ngfnrnhrfl
      301 hlswisshky ypfqgsa
//
LOCUS       XP_077505842             317 aa            linear   INV 25-NOV-2025
DEFINITION  aldo-keto reductase 1B-like [Amblyomma americanum].
ACCESSION   XP_077505842
VERSION     XP_077505842.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649716.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..317
                     /product="aldo-keto reductase 1B-like"
                     /calculated_mol_wt=35875
     Region          10..299
                     /region_name="AKR_SF"
                     /note="Aldo-keto reductase (AKR) superfamily; cl00470"
                     /db_xref="CDD:444925"
     Site            order(22..24,47,52,81,114,163..164,185,211..217,247,
                     262..265,270,273..274)
                     /site_type="active"
                     /db_xref="CDD:381296"
     Site            order(47,52,81,114)
                     /site_type="active"
                     /note="catalytic tetrad [active]"
                     /db_xref="CDD:381296"
     CDS             1..317
                     /gene="LOC144115359"
                     /coded_by="XM_077649716.1:178..1131"
                     /db_xref="GeneID:144115359"
ORIGIN      
        1 mtskvpfvkl ssgykiptig lgtwksqvgq vyeavkiaid agyrhidcaf ayqneeevgr
       61 aiadkikdgt verkdlwitg kcwntyhsrs kvfeccelsl kklcldyfdl ylmhwpmgyq
      121 eggemfpkne agdfifsdvd yletwaamee cvekglvrsi gvcnfnkdql lrllgaakik
      181 pamlqvechp ylnqsdlinf ckehdvavta ysplgspdrp wakagdpllm eepaikaiae
      241 ahgktaaqvl iryqiergvi vipksvtker iisnldvfdf kltpkemeti ngfnrnhrfl
      301 hlswisshky ypfqgsa
//
LOCUS       XP_077505843             136 aa            linear   INV 25-NOV-2025
DEFINITION  J domain-containing protein-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505843
VERSION     XP_077505843.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649717.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..136
                     /product="J domain-containing protein-like isoform X1"
                     /calculated_mol_wt=15738
     Region          8..61
                     /region_name="DnaJ"
                     /note="DnaJ domain; pfam00226"
                     /db_xref="CDD:395170"
     Site            order(27..29,38,41..42,45..46)
                     /site_type="other"
                     /note="HSP70 interaction site [polypeptide binding]"
                     /db_xref="CDD:99751"
     CDS             1..136
                     /gene="LOC144115360"
                     /coded_by="XM_077649717.1:16..426"
                     /db_xref="GeneID:144115360"
ORIGIN      
        1 mpgtnarpss qdeqllceyk vkalkfhpdk npgnpeaaqe fqriqeardv lldpqkrkky
       61 dkwrksglsm pfqqyvnlnv tslhwvtkkt kepmlmehgy qvpmetrvds sggapsswrs
      121 ketadellrk frnyei
//
LOCUS       XP_077505844             155 aa            linear   INV 25-NOV-2025
DEFINITION  dnaJ homolog subfamily C member 12-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505844
VERSION     XP_077505844.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649718.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..155
                     /product="dnaJ homolog subfamily C member 12-like isoform
                     X2"
                     /calculated_mol_wt=17959
     Region          18..80
                     /region_name="DnaJ"
                     /note="DnaJ domain; pfam00226"
                     /db_xref="CDD:395170"
     Site            order(46..48,57,60..61,64..65)
                     /site_type="other"
                     /note="HSP70 interaction site [polypeptide binding]"
                     /db_xref="CDD:99751"
     CDS             1..155
                     /gene="LOC144115360"
                     /coded_by="XM_077649718.1:50..517"
                     /db_xref="GeneID:144115360"
ORIGIN      
        1 meaflenvlk gpckaqvdyy evlgcdelst deqllceykv kalkfhpdkn pgnpeaaqef
       61 qriqeardvl ldpqkrkkyd kwrksglsmp fqqyvnlnvt slhwvtkktk epmlmehgyq
      121 vpmetrvdss ggapsswrsk etadellrkf rnyei
//
LOCUS       XP_077505845             739 aa            linear   INV 25-NOV-2025
DEFINITION  inhibitor of nuclear factor kappa-B kinase subunit alpha-like
            [Amblyomma americanum].
ACCESSION   XP_077505845
VERSION     XP_077505845.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649719.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..739
                     /product="inhibitor of nuclear factor kappa-B kinase
                     subunit alpha-like"
                     /calculated_mol_wt=83934
     Region          21..306
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(21..24,27,29,42,44,77,98..101,147,151..152,154,
                     167..168)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..739
                     /gene="LOC144115361"
                     /coded_by="XM_077649719.1:130..2349"
                     /db_xref="GeneID:144115361"
ORIGIN      
        1 mgkpteednv vigcwregrv ldrgtfgsit lwhntdtqet ialkkclrgt aemtdklqqr
       61 wvqeieilkk ldhpnvvkgl papeplqvla salpflamey csggnlrkvl qqpenccgll
      121 ekdvrrvlsd vtsglqylhs nhvihrdlkp enivlknidg ktvykiidlg yakefdqssl
      181 cnsfvgtlqy lapelfekkp ytravdlwsl gilayevitg qrpfsptasp aqwiplvhnk
      241 ksgdirgdia ltgervywrt lpaetcltsv fkedleewlq vllesqpekr srrggltife
      301 ilerllarpt vhifcitnml vvsasfesdt sttdflsqtl eratgvhttd qllltesgkv
      361 adiqyikdhc arlqesadvm lylidtsdds gkeqqsawle qlhgcttrls dnriylksqe
      421 vrrewalavh tmrqsmrtfq nvprafhsfq aylsseipal qkalssihve vnkltammki
      481 vhesytkdma clqewinlcs vkggpsadtd nilkswsncd ellsdvykqr kkveycfnkf
      541 nklctileds qrtpygqgtp leildeimar lwqlyekgkq krdydsvskt cmdmcgtire
      601 ldqeqervlk hvspymrsml wafhelgslh hsvgklsdki vsisralsve qeqrqqsiwn
      661 llhmetkrsl pvassaqnqv avpageslsw lslcqqmdeq vkesreqcne adaacelada
      721 niaklekecn ellksmttk
//
LOCUS       XP_077505846             739 aa            linear   INV 25-NOV-2025
DEFINITION  inhibitor of nuclear factor kappa-B kinase subunit alpha-like
            [Amblyomma americanum].
ACCESSION   XP_077505846
VERSION     XP_077505846.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649720.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..739
                     /product="inhibitor of nuclear factor kappa-B kinase
                     subunit alpha-like"
                     /calculated_mol_wt=83934
     Region          21..306
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(21..24,27,29,42,44,77,98..101,147,151..152,154,
                     167..168)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..739
                     /gene="LOC144115361"
                     /coded_by="XM_077649720.1:124..2343"
                     /db_xref="GeneID:144115361"
ORIGIN      
        1 mgkpteednv vigcwregrv ldrgtfgsit lwhntdtqet ialkkclrgt aemtdklqqr
       61 wvqeieilkk ldhpnvvkgl papeplqvla salpflamey csggnlrkvl qqpenccgll
      121 ekdvrrvlsd vtsglqylhs nhvihrdlkp enivlknidg ktvykiidlg yakefdqssl
      181 cnsfvgtlqy lapelfekkp ytravdlwsl gilayevitg qrpfsptasp aqwiplvhnk
      241 ksgdirgdia ltgervywrt lpaetcltsv fkedleewlq vllesqpekr srrggltife
      301 ilerllarpt vhifcitnml vvsasfesdt sttdflsqtl eratgvhttd qllltesgkv
      361 adiqyikdhc arlqesadvm lylidtsdds gkeqqsawle qlhgcttrls dnriylksqe
      421 vrrewalavh tmrqsmrtfq nvprafhsfq aylsseipal qkalssihve vnkltammki
      481 vhesytkdma clqewinlcs vkggpsadtd nilkswsncd ellsdvykqr kkveycfnkf
      541 nklctileds qrtpygqgtp leildeimar lwqlyekgkq krdydsvskt cmdmcgtire
      601 ldqeqervlk hvspymrsml wafhelgslh hsvgklsdki vsisralsve qeqrqqsiwn
      661 llhmetkrsl pvassaqnqv avpageslsw lslcqqmdeq vkesreqcne adaacelada
      721 niaklekecn ellksmttk
//
LOCUS       XP_077505847             222 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115366 [Amblyomma americanum].
ACCESSION   XP_077505847
VERSION     XP_077505847.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649721.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..222
                     /product="uncharacterized protein LOC144115366"
                     /calculated_mol_wt=25696
     Region          <56..218
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          60..80
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(60,63,76,80)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(65,67,69,71..72,75..76,79,93,95,99..100,103..104,
                     107,121,123,125,127..128,131..132,135)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          88..108
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          116..136
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          144..164
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          172..192
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          200..216
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..222
                     /gene="LOC144115366"
                     /coded_by="XM_077649721.1:4331..4999"
                     /db_xref="GeneID:144115366"
ORIGIN      
        1 mtpflhahrl edcmcssmvi qtghgaspgl dfshlshcme stagignrqs kptgkhryec
       61 thcdmefkkh snlmthlrvh tgerpyrceh cgknfsvkfa lqrhlrthtd erpfrcdhcg
      121 isfsenrdlv ahlhthtgeh pyrcdkcgrc fsmkstlamh irthtgekpy hchlcpmvfa
      181 dhsnlrrhvk shrgekpfhc dlcqktfsrr eglkhhkdvm hk
//
LOCUS       XP_077505848             905 aa            linear   INV 25-NOV-2025
DEFINITION  piwi-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077505848
VERSION     XP_077505848.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649722.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..905
                     /product="piwi-like protein 1"
                     /calculated_mol_wt=101790
     Region          323..455
                     /region_name="PAZ"
                     /note="This domain is named PAZ after the proteins Piwi
                     Argonaut and Zwille; smart00949"
                     /db_xref="CDD:198017"
     Site            order(364,377,384,388,406,421,423)
                     /site_type="other"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]"
                     /db_xref="CDD:239211"
     Region          442..888
                     /region_name="Piwi_piwi-like_Euk"
                     /note="PIWI domain, Piwi-like subfamily found in
                     eukaryotes. This domain is found in Piwi and closely
                     related proteins, where it is believed to perform a
                     crucial role in germline cells, via RNA silencing. RNA
                     silencing refers to a group of related...; cd04658"
                     /db_xref="CDD:240016"
     Site            order(611,615,627..630,633,643,646,650,654)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240016"
     Site            order(675,677,746,880)
                     /site_type="active"
                     /db_xref="CDD:240016"
     CDS             1..905
                     /gene="LOC144115367"
                     /coded_by="XM_077649722.1:995..3712"
                     /db_xref="GeneID:144115367"
ORIGIN      
        1 mdknsgdqal ppvgrargrs rgraravvvl pgaqaaptvp qfptamvpla alprepgvlr
       61 pamgslpsmp aldsvaglss sidmmaighr ahlqqmaama psavvpqqfp fppavtpgds
      121 gdggrpvgrr qsdefavvet rpahidnkrg tsgsainvva nyfrlismpq fcihqyhvef
      181 tptvessrmr rallvdhmhi fnkclvfdgm sdlksptrlq qditevfpqr rtdgkiicvr
      241 fkwvqelapi hpellrlfnt qmrrnlqrld fvqinrhffd rravssipqh glelwqglit
      301 aigqydsgil lvtdtlhkvl rrdsvydlms qiehvpnyke ecvkrvagci vitpynnkty
      361 rvddidwdnn pactfetkeg pktyadyyrd qyekhihdmr qpllvvrpkd kdlragrtqn
      421 iylvpelcfl tgltdemran vsvmrdlaqh trvepskrvr nllefmdrin nnaairndmd
      481 swgiqfddsl vtidarvlpp ekvmqgsnay rysaatanfs retrdrpmhv avaveswivl
      541 chrreeanvt efvrtlmsvc ppmgvkiaqp rlvlldddrp sgfvqglnql arggniqlal
      601 ivlpnnrkdr ydiikkeacv dlglhtqvil artignrkti hsvatkvavq lncklggeaw
      661 cleiplvstm visydtypds strnrsagaf vasinksltr wyqrvsfydt heglgkllps
      721 llrdalhkys qcndgaspdr iivfrdgvsd gqipqvkvwe idqilaslqa lfpgvehkla
      781 fvvvtksist rffvsgrehv tnpppgtvvd sevtrperyd fflvsqsvrq gtvapthynv
      841 iydttglkpd hmqrlayklt hlyfnwpgti rvpapcqyah klaflagqsl haehnprlss
      901 tlfyl
//
LOCUS       XP_077505849             905 aa            linear   INV 25-NOV-2025
DEFINITION  piwi-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077505849
VERSION     XP_077505849.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649723.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..905
                     /product="piwi-like protein 1"
                     /calculated_mol_wt=101790
     Region          323..455
                     /region_name="PAZ"
                     /note="This domain is named PAZ after the proteins Piwi
                     Argonaut and Zwille; smart00949"
                     /db_xref="CDD:198017"
     Site            order(364,377,384,388,406,421,423)
                     /site_type="other"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]"
                     /db_xref="CDD:239211"
     Region          442..888
                     /region_name="Piwi_piwi-like_Euk"
                     /note="PIWI domain, Piwi-like subfamily found in
                     eukaryotes. This domain is found in Piwi and closely
                     related proteins, where it is believed to perform a
                     crucial role in germline cells, via RNA silencing. RNA
                     silencing refers to a group of related...; cd04658"
                     /db_xref="CDD:240016"
     Site            order(611,615,627..630,633,643,646,650,654)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240016"
     Site            order(675,677,746,880)
                     /site_type="active"
                     /db_xref="CDD:240016"
     CDS             1..905
                     /gene="LOC144115367"
                     /coded_by="XM_077649723.1:4026..6743"
                     /db_xref="GeneID:144115367"
ORIGIN      
        1 mdknsgdqal ppvgrargrs rgraravvvl pgaqaaptvp qfptamvpla alprepgvlr
       61 pamgslpsmp aldsvaglss sidmmaighr ahlqqmaama psavvpqqfp fppavtpgds
      121 gdggrpvgrr qsdefavvet rpahidnkrg tsgsainvva nyfrlismpq fcihqyhvef
      181 tptvessrmr rallvdhmhi fnkclvfdgm sdlksptrlq qditevfpqr rtdgkiicvr
      241 fkwvqelapi hpellrlfnt qmrrnlqrld fvqinrhffd rravssipqh glelwqglit
      301 aigqydsgil lvtdtlhkvl rrdsvydlms qiehvpnyke ecvkrvagci vitpynnkty
      361 rvddidwdnn pactfetkeg pktyadyyrd qyekhihdmr qpllvvrpkd kdlragrtqn
      421 iylvpelcfl tgltdemran vsvmrdlaqh trvepskrvr nllefmdrin nnaairndmd
      481 swgiqfddsl vtidarvlpp ekvmqgsnay rysaatanfs retrdrpmhv avaveswivl
      541 chrreeanvt efvrtlmsvc ppmgvkiaqp rlvlldddrp sgfvqglnql arggniqlal
      601 ivlpnnrkdr ydiikkeacv dlglhtqvil artignrkti hsvatkvavq lncklggeaw
      661 cleiplvstm visydtypds strnrsagaf vasinksltr wyqrvsfydt heglgkllps
      721 llrdalhkys qcndgaspdr iivfrdgvsd gqipqvkvwe idqilaslqa lfpgvehkla
      781 fvvvtksist rffvsgrehv tnpppgtvvd sevtrperyd fflvsqsvrq gtvapthynv
      841 iydttglkpd hmqrlayklt hlyfnwpgti rvpapcqyah klaflagqsl haehnprlss
      901 tlfyl
//
LOCUS       XP_077505850             541 aa            linear   INV 25-NOV-2025
DEFINITION  F-box and WD repeat domain containing 5 [Amblyomma americanum].
ACCESSION   XP_077505850
VERSION     XP_077505850.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649724.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..541
                     /product="F-box and WD repeat domain containing 5"
                     /calculated_mol_wt=61201
     Region          2..46
                     /region_name="F-box-like"
                     /note="pfam12937"
                     /db_xref="CDD:463757"
     Region          82..115
                     /region_name="WD40"
                     /note="WD40 repeats; smart00320"
                     /db_xref="CDD:197651"
     Region          91..124
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          129..181
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <401..495
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          428..454
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          469..494
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..541
                     /gene="Fbw5"
                     /coded_by="XM_077649724.1:182..1807"
                     /db_xref="GeneID:144115368"
ORIGIN      
        1 mwdslpypll lrvlshlsar dicrcgcvcs swslaaddel lwrkkfqedw qvdpsipvlc
       61 garwkseyrr lyfeapvvea evlrdhdhqv lhvsfshdgt rfascskdgh vklwnaahpa
      121 kvaytrnmrd lcwnytqfsq fnesdtlllv sgvhfganta sgeiavfnlq eefvlqcrvl
      181 nkpydifgtw ytdhyllsgk lhflgqmlsc savwlnkawq ecesekkpiv kqlfkffnkn
      241 assvrtilva nceqpqvdgt papepsssle dddtdedsad nddpldprek llifttgslt
      301 yaphqvgfkr ikpfrfaepe kepptllqrl aeqqearrep pdpleqafdd vdhlvdmhgh
      361 iigmglspdh rylyinsrpw pqnyaiehal spppiaqeid ihvidlttmk eagnllrshr
      421 aytpndecff ifldvsekyv asgaedkkgy lwdrhyavcl aklphddvvn avafnpkdpe
      481 mlvtasddft lkvwrsraqa ralglapkvs mektvgsssv dasksgfrpg psgtskgwqs
      541 v
//
LOCUS       XP_077505851             376 aa            linear   INV 25-NOV-2025
DEFINITION  failed axon connections [Amblyomma americanum].
ACCESSION   XP_077505851
VERSION     XP_077505851.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649725.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..376
                     /product="failed axon connections"
                     /calculated_mol_wt=42171
     Region          61..135
                     /region_name="GST_N_Metaxin_like"
                     /note="GST_N family, Metaxin subfamily, Metaxin-like
                     proteins; a heterogenous group of proteins, predominantly
                     uncharacterized, with similarity to metaxins and GSTs.
                     Metaxin 1 is a component of a preprotein import complex of
                     the mitochondrial outer membrane. It...; cd03080"
                     /db_xref="CDD:239378"
     Site            order(79,81..82,84..85,88..89,124,127..128)
                     /site_type="other"
                     /note="putative C-terminal domain interface [polypeptide
                     binding]"
                     /db_xref="CDD:239378"
     Site            order(79,109..111,122..123)
                     /site_type="other"
                     /note="putative GSH binding site [chemical binding]"
                     /db_xref="CDD:239378"
     Site            order(109,121..122,124..125,128)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:239378"
     Region          229..291
                     /region_name="GST_C_6"
                     /note="Glutathione S-transferase, C-terminal domain;
                     pfam17171"
                     /db_xref="CDD:465369"
     CDS             1..376
                     /gene="fax"
                     /coded_by="XM_077649725.1:125..1255"
                     /db_xref="GeneID:144115369"
ORIGIN      
        1 mtspsetens agtappakae kpaeapatqe taasptsdat ttaasapakp svhktdyekd
       61 vvylyqftrc ptlpgispfc lkvetwlrmt qvkyenvdhk lkfkskkgql pfvelngeei
      121 adsdiiikql tqifgkdlda gltteernis hafssmlnnh tgwvsrcwry rhpgqflkaa
      181 qmdvkrmlns klpkgvlqff fklafksnvq qtighglgrh tteeilefgk edlkhlsqfl
      241 gtkpfffgte phlldcvafa hlcqfvyvpf gnlleymeqd canllafver mkerywadwd
      301 eicktlelnt hlpkkvpeee pkkaaesapa adekspaaed avkkseatve evkkeevikk
      361 evveevvtkv vtevke
//
LOCUS       XP_077505852             366 aa            linear   INV 25-NOV-2025
DEFINITION  coenzyme Q4 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505852
VERSION     XP_077505852.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649726.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..366
                     /product="coenzyme Q4 isoform X1"
                     /calculated_mol_wt=41975
     Region          73..284
                     /region_name="Coq4"
                     /note="Coenzyme Q (ubiquinone) biosynthesis protein Coq4;
                     pfam05019"
                     /db_xref="CDD:461522"
     CDS             1..366
                     /gene="Coq4"
                     /coded_by="XM_077649726.1:122..1222"
                     /db_xref="GeneID:144115370"
ORIGIN      
        1 mlrlpafvcy rrhsvkrsvy rlprflaqtp gcspllltar plaqassaet qghneplveq
       61 gplypghipt tfaqkalisv gsalaaitdp frddmvavfg evtgayafrd lhrimsndee
      121 grqilrdrpr intrtvdldy lsrlpdytfg ytyyrflhdn ivspdsrlpv qfvddaelay
      181 vaqryrevhd lihtilempi hmlgevtvkw veaihtkmpm cstgavfgai rlkpkqrrdy
      241 vnihlpwalr vgfnaknlmc vyyekhweep lselqqklgi epfprqtprq tdrgrrnspd
      301 qdvddtqhlh qmspinvagr gkhnhllgrg lpnnrahsrd rrrsatrwet gsatrwrrqn
      361 vsnvai
//
LOCUS       XP_077505853             305 aa            linear   INV 25-NOV-2025
DEFINITION  coenzyme Q4 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505853
VERSION     XP_077505853.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649727.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..305
                     /product="coenzyme Q4 isoform X2"
                     /calculated_mol_wt=34952
     Region          73..284
                     /region_name="Coq4"
                     /note="Coenzyme Q (ubiquinone) biosynthesis protein Coq4;
                     pfam05019"
                     /db_xref="CDD:461522"
     CDS             1..305
                     /gene="Coq4"
                     /coded_by="XM_077649727.1:121..1038"
                     /db_xref="GeneID:144115370"
ORIGIN      
        1 mlrlpafvcy rrhsvkrsvy rlprflaqtp gcspllltar plaqassaet qghneplveq
       61 gplypghipt tfaqkalisv gsalaaitdp frddmvavfg evtgayafrd lhrimsndee
      121 grqilrdrpr intrtvdldy lsrlpdytfg ytyyrflhdn ivspdsrlpv qfvddaelay
      181 vaqryrevhd lihtilempi hmlgevtvkw veaihtkmpm cstgavfgai rlkpkqrrdy
      241 vnihlpwalr vgfnaknlmc vyyekhweep lselqqklgi epfprqtprq tdrgersktw
      301 vdesg
//
LOCUS       XP_077505854             295 aa            linear   INV 25-NOV-2025
DEFINITION  coenzyme Q4 isoform X3 [Amblyomma americanum].
ACCESSION   XP_077505854
VERSION     XP_077505854.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649728.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..295
                     /product="coenzyme Q4 isoform X3"
                     /calculated_mol_wt=33805
     Region          73..284
                     /region_name="Coq4"
                     /note="Coenzyme Q (ubiquinone) biosynthesis protein Coq4;
                     pfam05019"
                     /db_xref="CDD:461522"
     CDS             1..295
                     /gene="Coq4"
                     /coded_by="XM_077649728.1:120..1007"
                     /db_xref="GeneID:144115370"
ORIGIN      
        1 mlrlpafvcy rrhsvkrsvy rlprflaqtp gcspllltar plaqassaet qghneplveq
       61 gplypghipt tfaqkalisv gsalaaitdp frddmvavfg evtgayafrd lhrimsndee
      121 grqilrdrpr intrtvdldy lsrlpdytfg ytyyrflhdn ivspdsrlpv qfvddaelay
      181 vaqryrevhd lihtilempi hmlgevtvkw veaihtkmpm cstgavfgai rlkpkqrrdy
      241 vnihlpwalr vgfnaknlmc vyyekhweep lselqqklgi epfprqtprq tdrgk
//
LOCUS       XP_077505855             272 aa            linear   INV 25-NOV-2025
DEFINITION  coenzyme Q4 isoform X4 [Amblyomma americanum].
ACCESSION   XP_077505855
VERSION     XP_077505855.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649729.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..272
                     /product="coenzyme Q4 isoform X4"
                     /calculated_mol_wt=31707
     Region          1..190
                     /region_name="Coq4"
                     /note="Coenzyme Q (ubiquinone) biosynthesis protein Coq4;
                     pfam05019"
                     /db_xref="CDD:461522"
     CDS             1..272
                     /gene="Coq4"
                     /coded_by="XM_077649729.1:280..1098"
                     /db_xref="GeneID:144115370"
ORIGIN      
        1 mvavfgevtg ayafrdlhri msndeegrqi lrdrprintr tvdldylsrl pdytfgytyy
       61 rflhdnivsp dsrlpvqfvd daelayvaqr yrevhdliht ilempihmlg evtvkwveai
      121 htkmpmcstg avfgairlkp kqrrdyvnih lpwalrvgfn aknlmcvyye khweeplsel
      181 qqklgiepfp rqtprqtdrg rrnspdqdvd dtqhlhqmsp invagrgkhn hllgrglpnn
      241 rahsrdrrrs atrwetgsat rwrrqnvsnv ai
//
LOCUS       XP_077505856             334 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial ribosome recycling factor 1 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505856
VERSION     XP_077505856.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649730.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..334
                     /product="mitochondrial ribosome recycling factor 1
                     isoform X1"
                     /calculated_mol_wt=37673
     Region          173..330
                     /region_name="RRF"
                     /note="Ribosome recycling factor; pfam01765"
                     /db_xref="CDD:460316"
     CDS             1..334
                     /gene="mRRF1"
                     /coded_by="XM_077649730.1:1..1005"
                     /db_xref="GeneID:144115371"
ORIGIN      
        1 mhqnateldn csslpmprmp qrskrrcrrt fsrthparvr erretaltas ssqsfyfwrt
       61 vlfsditaan csmalryqlq lvgrlrsfvp pcsavgtmls qhthqpvlih arkhppslis
      121 cchyakganr pkhttkenkp kialsddelv evirvhnfrs emqkvvqrlq esfikhltlh
      181 saagsidtip vhvdgqeysl aevaqiakkn pqlivlnmts fpdaikptiq aiqasglnis
      241 tqqdgttvfc hlpkitkehr eslaknaktl fakakedlls ierkyikeiq knkqnvsedl
      301 synaslqvkm eventinkad amtkakqqdl lgek
//
LOCUS       XP_077505857             334 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial ribosome recycling factor 1 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505857
VERSION     XP_077505857.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649731.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..334
                     /product="mitochondrial ribosome recycling factor 1
                     isoform X1"
                     /calculated_mol_wt=37673
     Region          173..330
                     /region_name="RRF"
                     /note="Ribosome recycling factor; pfam01765"
                     /db_xref="CDD:460316"
     CDS             1..334
                     /gene="mRRF1"
                     /coded_by="XM_077649731.1:1..1005"
                     /db_xref="GeneID:144115371"
ORIGIN      
        1 mhqnateldn csslpmprmp qrskrrcrrt fsrthparvr erretaltas ssqsfyfwrt
       61 vlfsditaan csmalryqlq lvgrlrsfvp pcsavgtmls qhthqpvlih arkhppslis
      121 cchyakganr pkhttkenkp kialsddelv evirvhnfrs emqkvvqrlq esfikhltlh
      181 saagsidtip vhvdgqeysl aevaqiakkn pqlivlnmts fpdaikptiq aiqasglnis
      241 tqqdgttvfc hlpkitkehr eslaknaktl fakakedlls ierkyikeiq knkqnvsedl
      301 synaslqvkm eventinkad amtkakqqdl lgek
//
LOCUS       XP_077505858             288 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial ribosome recycling factor 1 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505858
VERSION     XP_077505858.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649732.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..288
                     /product="mitochondrial ribosome recycling factor 1
                     isoform X2"
                     /calculated_mol_wt=32355
     Region          127..284
                     /region_name="RRF"
                     /note="Ribosome recycling factor; pfam01765"
                     /db_xref="CDD:460316"
     CDS             1..288
                     /gene="mRRF1"
                     /coded_by="XM_077649732.1:88..954"
                     /db_xref="GeneID:144115371"
ORIGIN      
        1 mfygyverrk rsfkglcacd rgdrctmalr yqlqlvgrlr sfvppcsavg tmlsqhthqp
       61 vliharkhpp sliscchyak ganrpkhttk enkpkialsd delvevirvh nfrsemqkvv
      121 qrlqesfikh ltlhsaagsi dtipvhvdgq eyslaevaqi akknpqlivl nmtsfpdaik
      181 ptiqaiqasg lnistqqdgt tvfchlpkit kehreslakn aktlfakake dllsierkyi
      241 keiqknkqnv sedlsynasl qvkmeventi nkadamtkak qqdllgek
//
LOCUS       XP_077505859             266 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial ribosome recycling factor 1 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505859
VERSION     XP_077505859.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649733.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..266
                     /product="mitochondrial ribosome recycling factor 1
                     isoform X3"
                     /calculated_mol_wt=29752
     Region          105..262
                     /region_name="RRF"
                     /note="Ribosome recycling factor; pfam01765"
                     /db_xref="CDD:460316"
     CDS             1..266
                     /gene="mRRF1"
                     /coded_by="XM_077649733.1:122..922"
                     /db_xref="GeneID:144115371"
ORIGIN      
        1 mfygmalryq lqlvgrlrsf vppcsavgtm lsqhthqpvl iharkhppsl iscchyakga
       61 nrpkhttken kpkialsdde lvevirvhnf rsemqkvvqr lqesfikhlt lhsaagsidt
      121 ipvhvdgqey slaevaqiak knpqlivlnm tsfpdaikpt iqaiqasgln istqqdgttv
      181 fchlpkitke hreslaknak tlfakakedl lsierkyike iqknkqnvse dlsynaslqv
      241 kmeventink adamtkakqq dllgek
//
LOCUS       XP_077505860             266 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial ribosome recycling factor 1 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505860
VERSION     XP_077505860.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649734.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..266
                     /product="mitochondrial ribosome recycling factor 1
                     isoform X4"
                     /calculated_mol_wt=29764
     Region          105..262
                     /region_name="RRF"
                     /note="Ribosome recycling factor; pfam01765"
                     /db_xref="CDD:460316"
     CDS             1..266
                     /gene="mRRF1"
                     /coded_by="XM_077649734.1:16..816"
                     /db_xref="GeneID:144115371"
ORIGIN      
        1 myesmalryq lqlvgrlrsf vppcsavgtm lsqhthqpvl iharkhppsl iscchyakga
       61 nrpkhttken kpkialsdde lvevirvhnf rsemqkvvqr lqesfikhlt lhsaagsidt
      121 ipvhvdgqey slaevaqiak knpqlivlnm tsfpdaikpt iqaiqasgln istqqdgttv
      181 fchlpkitke hreslaknak tlfakakedl lsierkyike iqknkqnvse dlsynaslqv
      241 kmeventink adamtkakqq dllgek
//
LOCUS       XP_077505862             295 aa            linear   INV 25-NOV-2025
DEFINITION  phytanoyl-CoA dioxygenase domain-containing protein 1-like isoform
            X1 [Amblyomma americanum].
ACCESSION   XP_077505862
VERSION     XP_077505862.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649736.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..295
                     /product="phytanoyl-CoA dioxygenase domain-containing
                     protein 1-like isoform X1"
                     /calculated_mol_wt=33794
     Region          18..261
                     /region_name="PhyH"
                     /note="Phytanoyl-CoA dioxygenase (PhyH); pfam05721"
                     /db_xref="CDD:399029"
     CDS             1..295
                     /gene="LOC144115373"
                     /coded_by="XM_077649736.1:125..1012"
                     /db_xref="GeneID:144115373"
ORIGIN      
        1 mqhqpdvkck mecyhewfhk nglisipdfl seqdvnqlkq ecerivdsie dskgqitvfs
       61 trnhegrnky fldsgdkira fyeedafdeh gnlvvsksrs inkighalhw lnpvfrrvtf
      121 sskvkalvke iglkdprlvq smyifknpei ggevtahqda tflytepskl iglwfpleda
      181 tmengclwyi pgshnsmsls rrfirnndpd gpllkmvdis vhdysnetfv pvpvckgscv
      241 vihgnvvhks ekncslfprp aytfhiidrd gseyspqnwl qpseelpfps lyens
//
LOCUS       XP_077505863             293 aa            linear   INV 25-NOV-2025
DEFINITION  phytanoyl-CoA dioxygenase domain-containing protein 1-like isoform
            X2 [Amblyomma americanum].
ACCESSION   XP_077505863
VERSION     XP_077505863.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649737.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..293
                     /product="phytanoyl-CoA dioxygenase domain-containing
                     protein 1-like isoform X2"
                     /calculated_mol_wt=33250
     Region          13..286
                     /region_name="PhyH"
                     /note="Ectoine hydroxylase-related dioxygenase,
                     phytanoyl-CoA dioxygenase (PhyH) family [Secondary
                     metabolites biosynthesis, transport and catabolism];
                     COG5285"
                     /db_xref="CDD:444095"
     CDS             1..293
                     /gene="LOC144115373"
                     /coded_by="XM_077649737.1:178..1059"
                     /db_xref="GeneID:144115373"
ORIGIN      
        1 mddsgdlsft tcveqfhkng lisipdflse qdvnqlkqec erivdsieds kgqitvfstr
       61 nhegrnkyfl dsgdkirafy eedafdehgn lvvsksrsin kighalhwln pvfrrvtfss
      121 kvkalvkeig lkdprlvqsm yifknpeigg evtahqdatf lytepsklig lwfpledatm
      181 engclwyipg shnsmslsrr firnndpdgp llkmvdisvh dysnetfvpv pvckgscvvi
      241 hgnvvhksek ncslfprpay tfhiidrdgs eyspqnwlqp seelpfpsly ens
//
LOCUS       XP_077505864             185 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115374 [Amblyomma americanum].
ACCESSION   XP_077505864
VERSION     XP_077505864.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649738.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..185
                     /product="uncharacterized protein LOC144115374"
                     /calculated_mol_wt=19959
     Region          55..177
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     Site            order(58..61,90..92,95,121,160)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260008"
     CDS             1..185
                     /gene="LOC144115374"
                     /coded_by="XM_077649738.1:42..599"
                     /db_xref="GeneID:144115374"
ORIGIN      
        1 mvlrdppval femdiflcrg pgrqqgdpqv lravvhfcdl araviqeelq dhllvytdgs
       61 vardscslaa aaaipalrly sqqhatflgs ptmaelmgir lgldllltlc ppmpgsallc
      121 dsraalsrlq sngrgtplvr eirsrierfa qrgcavraqw vpghcgiagn eeadylanrc
      181 tsttc
//
LOCUS       XP_077505865             116 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115376 [Amblyomma americanum].
ACCESSION   XP_077505865
VERSION     XP_077505865.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649739.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..116
                     /product="uncharacterized protein LOC144115376"
                     /calculated_mol_wt=12431
     CDS             1..116
                     /gene="LOC144115376"
                     /coded_by="XM_077649739.1:3967..4317"
                     /db_xref="GeneID:144115376"
ORIGIN      
        1 mrgiipilvl dlrsllpavi igrvhlvcpa vpqpakpept spltpvpspa lttrhvdmtt
       61 lqrpetpptm mlvkglqqpt lhgaafsgpr hlfpvpgywl kivptslhlg lqaesh
//
LOCUS       XP_077505867            1292 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115377 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505867
VERSION     XP_077505867.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649741.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1292
                     /product="uncharacterized protein LOC144115377 isoform X1"
                     /calculated_mol_wt=142436
     Region          24..81
                     /region_name="LOTUS"
                     /note="LOTUS is an uncharacterized small globular domain
                     found in Limkain b1, Oskar and Tudor-containing proteins 5
                     and 7; cd08824"
                     /db_xref="CDD:193585"
     Region          219..289
                     /region_name="LabA_like_C"
                     /note="C-terminal domain of LabA_like proteins; cl14879"
                     /db_xref="CDD:472713"
     Region          456..581
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     CDS             1..1292
                     /gene="LOC144115377"
                     /coded_by="XM_077649741.1:300..4178"
                     /db_xref="GeneID:144115377"
ORIGIN      
        1 mkteavrpag avgggqlaav ltntervkkn lrelltensr dgidlrefed iyykkfgsti
       61 dhkacgyqtf ehflvgmadv lslsrssagv mkaflnkkgv kpassniqpa dmmplkktty
      121 syripgrlpe pqvkekeees hweanimaqd aarsqlqqsp psvitftcss rtqgihsqle
      181 pslspvisft hsspehfddn ktalpkgtnd vqtrksglsp vlkwgikkvl lkyssgiwls
      241 dfalkyqevt ghelkpsdhg fnnmlqlmek ctdilyltrp cyngdfmvyp hidpnkafdt
      301 rilaafefli rssgsqgmtv delleayqek tgilicpsdl gfsrqtfvaf lagnphlsft
      361 sfgrgqfvlk whhdvkeaar irtgglaaae psspfenfgg iertargtla dqatsveaaf
      421 kralaeveas fghfssppld lpkspfqlqe glseakycav yvsqvyavdh fylqlkgpdt
      481 slrleklmnq mkvyhkydpr ekipedgvhv gipcaapyts sdgssawhrg lviavskcpr
      541 scqvlyvdyg slavvpmhrl rilrdeffdl paqairaslg hlvptspcgw tpeskhffik
      601 latvdhalmc kvykkegpvy svylcdtvae pelhfadclv deglarlcfe qavgtvsslh
      661 ssnpktegtq kaslasiprq isepvapkpa ehplrsvhsd rciftdhltq pgsgvpaghr
      721 aepacqmphf etgqadqsak kgrraghgda prlseateqs keirrsease atkrseatkr
      781 swslfteyvk ehawktsqak ktektvtrdq seatkrgcsm fpsqtteqds tykqvwatgn
      841 pqkgsvnlqa qgangctasg qaytfhhfyt psqaeildlc hsaspmqtas dvptepqvet
      901 rgphetkpvh nwtnqrkeng qalaanrvwt eghsppsgqt kvafgalnep vaqapvmppv
      961 dqkgrtpvep fvgsavapaa salqssvpvs ylpsqrslgq pctvehlvaa irvaasssgk
     1021 hlhpqasaks ldrsvdssag atasatssqq pegaqvqaag plpededfma vlqreldgpl
     1081 lprfvkpeyl ktghclmvln hlqepfitta nvcqllewts ddilqkleek hiefpvlvlr
     1141 rpedvllfnq vasggvpgvr iceqlpeqvs ffalenvidl lnlfehpsqr lrqevtrvln
     1201 sfdpaseywq cvsddsdted lqsleedlar lhaqrkellq elmtssndki mdelsrieas
     1261 irkvevlmsr kgiqphqpsl arckepenhl gm
//
LOCUS       XP_077505868            1273 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115377 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505868
VERSION     XP_077505868.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649742.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1273
                     /product="uncharacterized protein LOC144115377 isoform X2"
                     /calculated_mol_wt=140222
     Region          24..81
                     /region_name="LOTUS"
                     /note="LOTUS is an uncharacterized small globular domain
                     found in Limkain b1, Oskar and Tudor-containing proteins 5
                     and 7; cd08824"
                     /db_xref="CDD:193585"
     Region          437..562
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     CDS             1..1273
                     /gene="LOC144115377"
                     /coded_by="XM_077649742.1:300..4121"
                     /db_xref="GeneID:144115377"
ORIGIN      
        1 mkteavrpag avgggqlaav ltntervkkn lrelltensr dgidlrefed iyykkfgsti
       61 dhkacgyqtf ehflvgmadv lslsrssagv mkaflnkkgv kpassniqpa dmmplkktty
      121 syripgrlpe pqvkekeees hweanimaqd aarsqlqqsp psvitftcss rtqgihsqle
      181 pslspvisft hsspehfddn ktalpkgtnd vqtrksglsp vlkwgikkev tghelkpsdh
      241 gfnnmlqlme kctdilyltr pcyngdfmvy phidpnkafd trilaafefl irssgsqgmt
      301 vdelleayqe ktgilicpsd lgfsrqtfva flagnphlsf tsfgrgqfvl kwhhdvkeaa
      361 rirtgglaaa epsspfenfg giertargtl adqatsveaa fkralaevea sfghfssppl
      421 dlpkspfqlq eglseakyca vyvsqvyavd hfylqlkgpd tslrleklmn qmkvyhkydp
      481 rekipedgvh vgipcaapyt ssdgssawhr glviavskcp rscqvlyvdy gslavvpmhr
      541 lrilrdeffd lpaqairasl ghlvptspcg wtpeskhffi klatvdhalm ckvykkegpv
      601 ysvylcdtva epelhfadcl vdeglarlcf eqavgtvssl hssnpktegt qkaslasipr
      661 qisepvapkp aehplrsvhs drciftdhlt qpgsgvpagh raepacqmph fetgqadqsa
      721 kkgrraghgd aprlseateq skeirrseas eatkrseatk rswslfteyv kehawktsqa
      781 kktektvtrd qseatkrgcs mfpsqtteqd stykqvwatg npqkgsvnlq aqgangctas
      841 gqaytfhhfy tpsqaeildl chsaspmqta sdvptepqve trgphetkpv hnwtnqrken
      901 gqalaanrvw teghsppsgq tkvafgalne pvaqapvmpp vdqkgrtpve pfvgsavapa
      961 asalqssvpv sylpsqrslg qpctvehlva airvaasssg khlhpqasak sldrsvdssa
     1021 gatasatssq qpegaqvqaa gplpededfm avlqreldgp llprfvkpey lktghclmvl
     1081 nhlqepfitt anvcqllewt sddilqklee khiefpvlvl rrpedvllfn qvasggvpgv
     1141 riceqlpeqv sffalenvid llnlfehpsq rlrqevtrvl nsfdpaseyw qcvsddsdte
     1201 dlqsleedla rlhaqrkell qelmtssndk imdelsriea sirkvevlms rkgiqphqps
     1261 larckepenh lgm
//
LOCUS       XP_077505869            1298 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115378 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505869
VERSION     XP_077505869.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649743.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1298
                     /product="uncharacterized protein LOC144115378 isoform X1"
                     /calculated_mol_wt=143079
     Region          24..81
                     /region_name="LOTUS"
                     /note="LOTUS is an uncharacterized small globular domain
                     found in Limkain b1, Oskar and Tudor-containing proteins 5
                     and 7; cd08824"
                     /db_xref="CDD:193585"
     Region          219..289
                     /region_name="LabA_like_C"
                     /note="C-terminal domain of LabA_like proteins; cl14879"
                     /db_xref="CDD:472713"
     Region          456..581
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          <903..1085
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     CDS             1..1298
                     /gene="LOC144115378"
                     /coded_by="XM_077649743.1:339..4235"
                     /db_xref="GeneID:144115378"
ORIGIN      
        1 mkteavrpag avgggqlaav ltntervkkn lrelltensr dgidlrefed iyykkfgsti
       61 dhkacgyqtf ehflvgmadv lslsrssagv mkaflnkkgv kpassniqpa dmmplkkttc
      121 syripgrlpe pqvkekeees hweanimaqd aarsqlqqsp psvitftcss rtqgihsqle
      181 pslspvisft hsspehfddn ktalpkgtnd vqtrksglsp vlkwgikkvl lkyssgiwls
      241 dfalkyqevt ghelkpsdhg fnnmlqlmek ctdilyltrp cyngdfmvyp hidpnkafdt
      301 rilaafefli rssgsqgmtv delleayqek tgilicpsdl gfsrqtfvaf lagnphlsft
      361 slgrgqfvlk whhdvkeaar irtgglaaae psspfenfgg iertargtla dqatsveaaf
      421 kralaeveas fghfssppld lpkspfqlqe glseakycav yvsqvyavdh fylqlkgpdt
      481 slrleklmnq mkvyhkydpr ekipedgvhv gipcaapyts sdgssawhrg lviavskcpr
      541 scqvlyvdyg slavvpmhrl rilrdeffdl paqairaslg hlvptspcgw tpeskhffik
      601 latvdhalmc kvykkegpvy svylcdtvae pelhfadclv deglarlcfe qavgtvsslh
      661 ssnpktegtq kaslasiprq isepvapkpa qhplrsvhsd rciftdhltq pgsgvpaghr
      721 aepacrmphf etgqadqsak kgrraghgda prlseateqs keirrseatk rseatkrsws
      781 lfteyvkeha wktsqakkte ktvtrdqsea tkrgcsmfps qtteqdstyk qvwatgnpqk
      841 gsvnlqaqga ngctasgqay tfhhfytpsq aetldrchsa spmqtasdvp tepqvetrgp
      901 hetkpvhnwt nqrkengqal aanrvwtegh sppsgqtkva fgalnepvaq apvmppvdqk
      961 gqtpvepfvg savapaasal qssvpvsylp sqrslgqpct vehlvaairv aasssgkhlh
     1021 pqasaksldr svespagata satssqqpeg aqvqaagplp ededfmavlq reldgplppr
     1081 fvkpeylktg hclmvlnhlq epfittanvc qllewtsddi lqkleekhie fpllvlrrpe
     1141 dvllfnqvas ggvpgvrice qlpeqvsffp lenvidllnl fehpsqrlrq evtrvlnsfd
     1201 paseywqcvs ddsdtedlqs leedlarlha qrkellqelm mtssndkimd elsriearir
     1261 kvevlmsrkv haatcktwtk kqarlqagsp tsssdqls
//
LOCUS       XP_077505870            1290 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115378 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505870
VERSION     XP_077505870.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649744.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1290
                     /product="uncharacterized protein LOC144115378 isoform X2"
                     /calculated_mol_wt=142292
     Region          24..81
                     /region_name="LOTUS"
                     /note="LOTUS is an uncharacterized small globular domain
                     found in Limkain b1, Oskar and Tudor-containing proteins 5
                     and 7; cd08824"
                     /db_xref="CDD:193585"
     Region          219..289
                     /region_name="LabA_like_C"
                     /note="C-terminal domain of LabA_like proteins; cl14879"
                     /db_xref="CDD:472713"
     Region          456..581
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          <903..1085
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     CDS             1..1290
                     /gene="LOC144115378"
                     /coded_by="XM_077649744.1:876..4748"
                     /db_xref="GeneID:144115378"
ORIGIN      
        1 mkteavrpag avgggqlaav ltntervkkn lrelltensr dgidlrefed iyykkfgsti
       61 dhkacgyqtf ehflvgmadv lslsrssagv mkaflnkkgv kpassniqpa dmmplkkttc
      121 syripgrlpe pqvkekeees hweanimaqd aarsqlqqsp psvitftcss rtqgihsqle
      181 pslspvisft hsspehfddn ktalpkgtnd vqtrksglsp vlkwgikkvl lkyssgiwls
      241 dfalkyqevt ghelkpsdhg fnnmlqlmek ctdilyltrp cyngdfmvyp hidpnkafdt
      301 rilaafefli rssgsqgmtv delleayqek tgilicpsdl gfsrqtfvaf lagnphlsft
      361 slgrgqfvlk whhdvkeaar irtgglaaae psspfenfgg iertargtla dqatsveaaf
      421 kralaeveas fghfssppld lpkspfqlqe glseakycav yvsqvyavdh fylqlkgpdt
      481 slrleklmnq mkvyhkydpr ekipedgvhv gipcaapyts sdgssawhrg lviavskcpr
      541 scqvlyvdyg slavvpmhrl rilrdeffdl paqairaslg hlvptspcgw tpeskhffik
      601 latvdhalmc kvykkegpvy svylcdtvae pelhfadclv deglarlcfe qavgtvsslh
      661 ssnpktegtq kaslasiprq isepvapkpa qhplrsvhsd rciftdhltq pgsgvpaghr
      721 aepacrmphf etgqadqsak kgrraghgda prlseateqs keirrseatk rseatkrsws
      781 lfteyvkeha wktsqakkte ktvtrdqsea tkrgcsmfps qtteqdstyk qvwatgnpqk
      841 gsvnlqaqga ngctasgqay tfhhfytpsq aetldrchsa spmqtasdvp tepqvetrgp
      901 hetkpvhnwt nqrkengqal aanrvwtegh sppsgqtkva fgalnepvaq apvmppvdqk
      961 gqtpvepfvg savapaasal qssvpvsylp sqrslgqpct vehlvaairv aasssgkhlh
     1021 pqasaksldr svespagata satssqqpeg aqvqaagplp ededfmavlq reldgplppr
     1081 fvkpeylktg hclmvlnhlq epfittanvc qllewtsddi lqkleekhie fpllvlrrpe
     1141 dvllfnqvas ggvpgvrice qlpeqvsffp lenvidllnl fehpsqrlrq evtrvlnsfd
     1201 paseywqcvs ddsdtedlqs leedlarlha qrkellqelm mtssndkimd elsriearir
     1261 kvevlmsrkg iqppqpslar ckepknhlgm
//
LOCUS       XP_077505871            1279 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115378 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505871
VERSION     XP_077505871.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649745.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1279
                     /product="uncharacterized protein LOC144115378 isoform X3"
                     /calculated_mol_wt=140865
     Region          24..81
                     /region_name="LOTUS"
                     /note="LOTUS is an uncharacterized small globular domain
                     found in Limkain b1, Oskar and Tudor-containing proteins 5
                     and 7; cd08824"
                     /db_xref="CDD:193585"
     Region          437..562
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          <884..1066
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     CDS             1..1279
                     /gene="LOC144115378"
                     /coded_by="XM_077649745.1:259..4098"
                     /db_xref="GeneID:144115378"
ORIGIN      
        1 mkteavrpag avgggqlaav ltntervkkn lrelltensr dgidlrefed iyykkfgsti
       61 dhkacgyqtf ehflvgmadv lslsrssagv mkaflnkkgv kpassniqpa dmmplkkttc
      121 syripgrlpe pqvkekeees hweanimaqd aarsqlqqsp psvitftcss rtqgihsqle
      181 pslspvisft hsspehfddn ktalpkgtnd vqtrksglsp vlkwgikkev tghelkpsdh
      241 gfnnmlqlme kctdilyltr pcyngdfmvy phidpnkafd trilaafefl irssgsqgmt
      301 vdelleayqe ktgilicpsd lgfsrqtfva flagnphlsf tslgrgqfvl kwhhdvkeaa
      361 rirtgglaaa epsspfenfg giertargtl adqatsveaa fkralaevea sfghfssppl
      421 dlpkspfqlq eglseakyca vyvsqvyavd hfylqlkgpd tslrleklmn qmkvyhkydp
      481 rekipedgvh vgipcaapyt ssdgssawhr glviavskcp rscqvlyvdy gslavvpmhr
      541 lrilrdeffd lpaqairasl ghlvptspcg wtpeskhffi klatvdhalm ckvykkegpv
      601 ysvylcdtva epelhfadcl vdeglarlcf eqavgtvssl hssnpktegt qkaslasipr
      661 qisepvapkp aqhplrsvhs drciftdhlt qpgsgvpagh raepacrmph fetgqadqsa
      721 kkgrraghgd aprlseateq skeirrseat krseatkrsw slfteyvkeh awktsqakkt
      781 ektvtrdqse atkrgcsmfp sqtteqdsty kqvwatgnpq kgsvnlqaqg angctasgqa
      841 ytfhhfytps qaetldrchs aspmqtasdv ptepqvetrg phetkpvhnw tnqrkengqa
      901 laanrvwteg hsppsgqtkv afgalnepva qapvmppvdq kgqtpvepfv gsavapaasa
      961 lqssvpvsyl psqrslgqpc tvehlvaair vaasssgkhl hpqasaksld rsvespagat
     1021 asatssqqpe gaqvqaagpl pededfmavl qreldgplpp rfvkpeylkt ghclmvlnhl
     1081 qepfittanv cqllewtsdd ilqkleekhi efpllvlrrp edvllfnqva sggvpgvric
     1141 eqlpeqvsff plenvidlln lfehpsqrlr qevtrvlnsf dpaseywqcv sddsdtedlq
     1201 sleedlarlh aqrkellqel mmtssndkim delsrieari rkvevlmsrk vhaatcktwt
     1261 kkqarlqags ptsssdqls
//
LOCUS       XP_077505872            1271 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115378 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505872
VERSION     XP_077505872.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649746.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1271
                     /product="uncharacterized protein LOC144115378 isoform X4"
                     /calculated_mol_wt=140078
     Region          24..81
                     /region_name="LOTUS"
                     /note="LOTUS is an uncharacterized small globular domain
                     found in Limkain b1, Oskar and Tudor-containing proteins 5
                     and 7; cd08824"
                     /db_xref="CDD:193585"
     Region          437..562
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          <884..1066
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     CDS             1..1271
                     /gene="LOC144115378"
                     /coded_by="XM_077649746.1:259..4074"
                     /db_xref="GeneID:144115378"
ORIGIN      
        1 mkteavrpag avgggqlaav ltntervkkn lrelltensr dgidlrefed iyykkfgsti
       61 dhkacgyqtf ehflvgmadv lslsrssagv mkaflnkkgv kpassniqpa dmmplkkttc
      121 syripgrlpe pqvkekeees hweanimaqd aarsqlqqsp psvitftcss rtqgihsqle
      181 pslspvisft hsspehfddn ktalpkgtnd vqtrksglsp vlkwgikkev tghelkpsdh
      241 gfnnmlqlme kctdilyltr pcyngdfmvy phidpnkafd trilaafefl irssgsqgmt
      301 vdelleayqe ktgilicpsd lgfsrqtfva flagnphlsf tslgrgqfvl kwhhdvkeaa
      361 rirtgglaaa epsspfenfg giertargtl adqatsveaa fkralaevea sfghfssppl
      421 dlpkspfqlq eglseakyca vyvsqvyavd hfylqlkgpd tslrleklmn qmkvyhkydp
      481 rekipedgvh vgipcaapyt ssdgssawhr glviavskcp rscqvlyvdy gslavvpmhr
      541 lrilrdeffd lpaqairasl ghlvptspcg wtpeskhffi klatvdhalm ckvykkegpv
      601 ysvylcdtva epelhfadcl vdeglarlcf eqavgtvssl hssnpktegt qkaslasipr
      661 qisepvapkp aqhplrsvhs drciftdhlt qpgsgvpagh raepacrmph fetgqadqsa
      721 kkgrraghgd aprlseateq skeirrseat krseatkrsw slfteyvkeh awktsqakkt
      781 ektvtrdqse atkrgcsmfp sqtteqdsty kqvwatgnpq kgsvnlqaqg angctasgqa
      841 ytfhhfytps qaetldrchs aspmqtasdv ptepqvetrg phetkpvhnw tnqrkengqa
      901 laanrvwteg hsppsgqtkv afgalnepva qapvmppvdq kgqtpvepfv gsavapaasa
      961 lqssvpvsyl psqrslgqpc tvehlvaair vaasssgkhl hpqasaksld rsvespagat
     1021 asatssqqpe gaqvqaagpl pededfmavl qreldgplpp rfvkpeylkt ghclmvlnhl
     1081 qepfittanv cqllewtsdd ilqkleekhi efpllvlrrp edvllfnqva sggvpgvric
     1141 eqlpeqvsff plenvidlln lfehpsqrlr qevtrvlnsf dpaseywqcv sddsdtedlq
     1201 sleedlarlh aqrkellqel mmtssndkim delsrieari rkvevlmsrk giqppqpsla
     1261 rckepknhlg m
//
LOCUS       XP_077505873            1237 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115380 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505873
VERSION     XP_077505873.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649747.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1237
                     /product="uncharacterized protein LOC144115380 isoform X1"
                     /calculated_mol_wt=136302
     Region          192..261
                     /region_name="LabA_like_C"
                     /note="C-terminal domain of LabA_like proteins; cl14879"
                     /db_xref="CDD:472713"
     Region          428..553
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     CDS             1..1237
                     /gene="LOC144115380"
                     /coded_by="XM_077649747.1:869..4582"
                     /db_xref="GeneID:144115380"
ORIGIN      
        1 madvlslsws sagvmkafln kkgaiqssgr akkavqrfkg hqsnpakkmt kpthlpsqrn
       61 wmpcqqqmkt hktlkpassn iqpadmmplk kttysyripg prepqvkeee kesdreaeim
      121 aqdaalsqlq qsppsvitft rsnhtqgihs qlepslspvi sfthssperf ddnktalpkg
      181 tndvqtrksg lspvlkrgik kvlskysggi wlsdlaseyk evtghelkpn dhglnnmlql
      241 mekctdilyf trpcyngdfm vcphtdvnka fdtrtlaafe flirssgsqg mtvdelleay
      301 qektgilicp sdlgfsrqtf vdflagnphl sftslgrgqf vlkwhhdvee aarirtggla
      361 aaepssplkn fgikrtargt ladqatsvda afqralaeve asfeqfsspp ldlpkspfql
      421 qeglseakyc avyvsqvyav dhfylqlkgp dtslrlqelm nqlevyhkyd prekipeedv
      481 yvgmpcavsy tysdgasdwh rglviavsec pplcqvlyvd ygslsvvpkk elrilrneff
      541 dlpaqairas lghlvptsps gwtpeskdff iklatvdhal mckvykkegp vysvylcdtv
      601 aepelhfadc lvdeglarlc feqavgtvss lhssnpkteg tqkaslasip rqisepvapk
      661 paqhplrsvh sdrciftdhl tqpgsgvpag hhaepacrmp hfetgqadqs akkgrraghg
      721 daprlseate qskeirrsea tkrseatkrs wslftdyvke hawktsqakk tektvtrdqs
      781 eatkrgcsmf psqtteqdst ykqvwatgnp qkgsvnlqaq gangctasgq aytfhapsqa
      841 etldrchsas pmqtasdvpt epqvetrgph etkpvhnwtn qrkengqala anrgwteghs
      901 ppsgqtkvaf galnepvaqa pvmppvgqkg qtpvepfvws avapaasalq ssvpvsylps
      961 qrslgqpgav elvvlaaira aasssgkhlh pqasaksldc svdssagata satssqqpeg
     1021 aqvqaagplp ededfmavlq reldgplppr fvkpeylrtg hclmvlnhlq kpfittanvc
     1081 qllewtsddi lqklekkciq fsvlvlrrpe ngllfnqmss ygvpgvrice qlqeqvsffa
     1141 lenvidllnl fehpsqrlrq evtrvlnsfd paseywqcis ddsdtedlqs leedlarlha
     1201 qrmeliqelv vssnekivde isrieatirk vvalmye
//
LOCUS       XP_077505874            1148 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115380 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505874
VERSION     XP_077505874.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649748.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1148
                     /product="uncharacterized protein LOC144115380 isoform X2"
                     /calculated_mol_wt=125893
     Region          192..261
                     /region_name="LabA_like_C"
                     /note="C-terminal domain of LabA_like proteins; cl14879"
                     /db_xref="CDD:472713"
     Region          428..553
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     CDS             1..1148
                     /gene="LOC144115380"
                     /coded_by="XM_077649748.1:869..4315"
                     /db_xref="GeneID:144115380"
ORIGIN      
        1 madvlslsws sagvmkafln kkgaiqssgr akkavqrfkg hqsnpakkmt kpthlpsqrn
       61 wmpcqqqmkt hktlkpassn iqpadmmplk kttysyripg prepqvkeee kesdreaeim
      121 aqdaalsqlq qsppsvitft rsnhtqgihs qlepslspvi sfthssperf ddnktalpkg
      181 tndvqtrksg lspvlkrgik kvlskysggi wlsdlaseyk evtghelkpn dhglnnmlql
      241 mekctdilyf trpcyngdfm vcphtdvnka fdtrtlaafe flirssgsqg mtvdelleay
      301 qektgilicp sdlgfsrqtf vdflagnphl sftslgrgqf vlkwhhdvee aarirtggla
      361 aaepssplkn fgikrtargt ladqatsvda afqralaeve asfeqfsspp ldlpkspfql
      421 qeglseakyc avyvsqvyav dhfylqlkgp dtslrlqelm nqlevyhkyd prekipeedv
      481 yvgmpcavsy tysdgasdwh rglviavsec pplcqvlyvd ygslsvvpkk elrilrneff
      541 dlpaqairas lghlvptsps gwtpeskdff iklatvdhal mckvykkegp vysvylcdtv
      601 aepelhfadc lvdeglarlc feqavgtvss lhssnpkteg tqkaslasip rqisepvapk
      661 paqhplrsvh sdrciftdhl tqpgsgvpag hhaepacrmp hfetgqadqs akkgrraghg
      721 daprlseate qskeirrsea tkrseatkrs wslftdyvke hawktsqakk tektvtrdqs
      781 eatkrgcsmf psqtteqdst ykqvwatgnp qkgsvnlqaq gangctasgq aytfhapsqa
      841 etldrchsas pmqtasdvpt epqvetrgph etkpvhnwtn qrkengqala anrgwteghs
      901 ppsgqtkvaf galnepvaqa pvmppvgqkg qtpvepfvws avapaasalq ssvpvsylps
      961 qrslgqpgav elvvlaaira aasssgkhlh pqasaksldc svdssagata satssqqpeg
     1021 aqvqaagplp ededfmavlq reldgplppr fvkpeylrtg hclmvlnhlq kpfittanvc
     1081 qllewtsddi lqklekkciq fsvlvlrrpe ngllfnqmss ygvpgvrice qlqeqvsffa
     1141 lendscsr
//
LOCUS       XP_077505875             265 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115381 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505875
VERSION     XP_077505875.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649749.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..265
                     /product="uncharacterized protein LOC144115381 isoform X1"
                     /calculated_mol_wt=30028
     CDS             1..265
                     /gene="LOC144115381"
                     /coded_by="XM_077649749.1:198..995"
                     /db_xref="GeneID:144115381"
ORIGIN      
        1 mehlrppepl rltadggrnw rlfrqkfeff lqatactdkp rseaaktall lsvagedald
       61 vfnnfvfteg eskedyatvi akfeeycveq qneiherasp tedgrspgel lqgrrlrtpv
      121 pdfnplptvr vkkhrqishn eralprlsnn dvvrvngksw srkakvvgsa gprafhvhte
      181 dntilrrnrr hllatkeqws dhssddedfg dnrassqgva stapvapptp aeqsalqplr
      241 rttrqmrppe rlgydqhfqq tsqrc
//
LOCUS       XP_077505876             112 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115381 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505876
VERSION     XP_077505876.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649750.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..112
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..112
                     /product="uncharacterized protein LOC144115381 isoform X2"
                     /calculated_mol_wt=12347
     CDS             1..112
                     /gene="LOC144115381"
                     /coded_by="XM_077649750.1:48..386"
                     /db_xref="GeneID:144115381"
ORIGIN      
        1 msqaepivke fprlfhgigr apreykivlh kealpvvqpa rrvplslrep lraeldrmek
       61 egiiqkvssp tdwmaladml srspapdhka padtcdvevh avtvvsalgk sy
//
LOCUS       XP_077505878             318 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115382 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505878
VERSION     XP_077505878.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649752.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..318
                     /product="uncharacterized protein LOC144115382 isoform X1"
                     /calculated_mol_wt=36771
     CDS             1..318
                     /gene="LOC144115382"
                     /coded_by="XM_077649752.1:91..1047"
                     /db_xref="GeneID:144115382"
ORIGIN      
        1 merrmsdaik aatedwsedd fakrpellkl feklkergey lvtndtrrkl gkakadglaa
       61 rerhlrallv kntwtdigks fcsnenlqpc megtakefte qfeglkaqdl iaeshsgsvs
      121 kvqsqpevke efkaeirill aeellkqksk llkqmgdfkc sssqesvvsk cidhnvkkrs
      181 vfnlvreyad casefkkaye eyltlvssmp vldeqqhhll kickevyeny ffanddveee
      241 vesciqrnvn hlfkeaekrl lceveqkrat vqqkesrlqe feriwspehe dkitsykiai
      301 ednrrlklal dtassdsd
//
LOCUS       XP_077505879             228 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115382 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505879
VERSION     XP_077505879.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649753.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..228
                     /product="uncharacterized protein LOC144115382 isoform X2"
                     /calculated_mol_wt=26363
     CDS             1..228
                     /gene="LOC144115382"
                     /coded_by="XM_077649753.1:508..1194"
                     /db_xref="GeneID:144115382"
ORIGIN      
        1 megtakefte qfeglkaqdl iaeshsgsvs kvqsqpevke efkaeirill aeellkqksk
       61 llkqmgdfkc sssqesvvsk cidhnvkkrs vfnlvreyad casefkkaye eyltlvssmp
      121 vldeqqhhll kickevyeny ffanddveee vesciqrnvn hlfkeaekrl lceveqkrat
      181 vqqkesrlqe feriwspehe dkitsykiai ednrrlklal dtassdsd
//
LOCUS       XP_077505880             228 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115382 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505880
VERSION     XP_077505880.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649754.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..228
                     /product="uncharacterized protein LOC144115382 isoform X2"
                     /calculated_mol_wt=26363
     CDS             1..228
                     /gene="LOC144115382"
                     /coded_by="XM_077649754.1:532..1218"
                     /db_xref="GeneID:144115382"
ORIGIN      
        1 megtakefte qfeglkaqdl iaeshsgsvs kvqsqpevke efkaeirill aeellkqksk
       61 llkqmgdfkc sssqesvvsk cidhnvkkrs vfnlvreyad casefkkaye eyltlvssmp
      121 vldeqqhhll kickevyeny ffanddveee vesciqrnvn hlfkeaekrl lceveqkrat
      181 vqqkesrlqe feriwspehe dkitsykiai ednrrlklal dtassdsd
//
LOCUS       XP_077505881             228 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115382 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505881
VERSION     XP_077505881.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649755.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..228
                     /product="uncharacterized protein LOC144115382 isoform X2"
                     /calculated_mol_wt=26363
     CDS             1..228
                     /gene="LOC144115382"
                     /coded_by="XM_077649755.1:255..941"
                     /db_xref="GeneID:144115382"
ORIGIN      
        1 megtakefte qfeglkaqdl iaeshsgsvs kvqsqpevke efkaeirill aeellkqksk
       61 llkqmgdfkc sssqesvvsk cidhnvkkrs vfnlvreyad casefkkaye eyltlvssmp
      121 vldeqqhhll kickevyeny ffanddveee vesciqrnvn hlfkeaekrl lceveqkrat
      181 vqqkesrlqe feriwspehe dkitsykiai ednrrlklal dtassdsd
//
LOCUS       XP_077505882             228 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115382 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505882
VERSION     XP_077505882.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649756.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..228
                     /product="uncharacterized protein LOC144115382 isoform X2"
                     /calculated_mol_wt=26363
     CDS             1..228
                     /gene="LOC144115382"
                     /coded_by="XM_077649756.1:236..922"
                     /db_xref="GeneID:144115382"
ORIGIN      
        1 megtakefte qfeglkaqdl iaeshsgsvs kvqsqpevke efkaeirill aeellkqksk
       61 llkqmgdfkc sssqesvvsk cidhnvkkrs vfnlvreyad casefkkaye eyltlvssmp
      121 vldeqqhhll kickevyeny ffanddveee vesciqrnvn hlfkeaekrl lceveqkrat
      181 vqqkesrlqe feriwspehe dkitsykiai ednrrlklal dtassdsd
//
LOCUS       XP_077505883             294 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115385 [Amblyomma americanum].
ACCESSION   XP_077505883
VERSION     XP_077505883.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649757.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 29% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..294
                     /product="uncharacterized protein LOC144115385"
                     /calculated_mol_wt=33145
     Region          63..145
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     CDS             1..294
                     /gene="LOC144115385"
                     /coded_by="XM_077649757.1:1..885"
                     /db_xref="GeneID:144115385"
ORIGIN      
        1 mpeaahhqav qvgslglcsg slrqrdpalf sgtdnrdaed wltsyerlst ynnwddttkl
       61 nnvffyltgv anlwfrnhea dlstwtafka nltevfggpa vrklraehrf rersrqsget
      121 ftsyiedvvn lcsrvnpqms eedkikhilk iiedddfqml lsrdlrtvae vvslcqsyde
      181 lrkqrlitrq haaavvesla glsaapdrsq llvhmkdfvr eevagqllll pspsvqspfl
      241 apdlptaise qlqplqcppr pfdcvgiyiy gplpytaagn rwvivaidhl trmq
//
LOCUS       XP_077505884             201 aa            linear   INV 25-NOV-2025
DEFINITION  peptidyl-prolyl cis-trans isomerase-like [Amblyomma americanum].
ACCESSION   XP_077505884
VERSION     XP_077505884.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649758.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..201
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..201
                     /product="peptidyl-prolyl cis-trans isomerase-like"
                     /calculated_mol_wt=21354
     Region          40..198
                     /region_name="cyclophilin_ABH_like"
                     /note="Cyclophilin A, B and H-like cyclophilin-type
                     peptidylprolyl cis- trans isomerase (PPIase) domain. This
                     family represents the archetypal cystolic cyclophilin
                     similar to human cyclophilins A, B and H. PPIase is an
                     enzyme which accelerates protein folding...; cd01926"
                     /db_xref="CDD:238907"
     Site            order(90..91,96,147,149,157)
                     /site_type="active"
                     /db_xref="CDD:238907"
     CDS             1..201
                     /gene="LOC144115386"
                     /coded_by="XM_077649758.1:176..781"
                     /db_xref="GeneID:144115386"
ORIGIN      
        1 mahvrvaiss ffspkagilk lpliplscsf ataagkmglp rvffdvtadg splgriviel
       61 rsdvapktae nfralctgek gfgysgstfh rvipnfmcqg gdftkhngtg gksiygekfe
      121 denfilkhtg pgilsmanag hntngsqffl ttvktpwldg khvvfgsvve gmdvvkkmes
      181 ygsqsgktsk klvvqssgql s
//
LOCUS       XP_077505885             209 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115387 [Amblyomma americanum].
ACCESSION   XP_077505885
VERSION     XP_077505885.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649759.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..209
                     /product="uncharacterized protein LOC144115387"
                     /calculated_mol_wt=23833
     CDS             1..209
                     /gene="LOC144115387"
                     /coded_by="XM_077649759.1:246..875"
                     /db_xref="GeneID:144115387"
ORIGIN      
        1 mqtrdamfsr isiirpadds wlssercesg psndvlskir farqddgrts sercespaas
       61 ggtpsvrspq addqnatest psctassmee eiqnlssvkd leerhirhlf nkvspvldqi
      121 grrevyswrl etyikggrdk ialthrvpih kfstkvtdqi lylfayefgk rrgfdgdiin
      181 qvfipeflvr ifmecqgttf eeserlmwq
//
LOCUS       XP_077505886              72 aa            linear   INV 25-NOV-2025
DEFINITION  defensin-1-like [Amblyomma americanum].
ACCESSION   XP_077505886
VERSION     XP_077505886.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649760.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..72
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..72
                     /product="defensin-1-like"
                     /calculated_mol_wt=7763
     Region          <36..71
                     /region_name="Defensin_2"
                     /note="Arthropod defensin; cl03093"
                     /db_xref="CDD:470736"
     CDS             1..72
                     /gene="LOC144115388"
                     /coded_by="XM_077649760.1:111..329"
                     /db_xref="GeneID:144115388"
ORIGIN      
        1 mkvlavafif vlvaglvsta deedksqvpl vrvrrgfgcp fnqyqchshc lsigrrggyc
       61 ggsfkttctc yn
//
LOCUS       XP_077505887              72 aa            linear   INV 25-NOV-2025
DEFINITION  defensin-1-like [Amblyomma americanum].
ACCESSION   XP_077505887
VERSION     XP_077505887.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649761.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by mRNA evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..72
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..72
                     /product="defensin-1-like"
                     /calculated_mol_wt=7749
     Region          <36..71
                     /region_name="Defensin_2"
                     /note="Arthropod defensin; cl03093"
                     /db_xref="CDD:470736"
     CDS             1..72
                     /gene="LOC144115389"
                     /coded_by="XM_077649761.1:94..312"
                     /db_xref="GeneID:144115389"
ORIGIN      
        1 mkvlavafif vlvgglvsta deedksqvpl vrvrrgfgcp fnqyqchshc lsigrrggyc
       61 ggsfkttctc yn
//
LOCUS       XP_077505888              72 aa            linear   INV 25-NOV-2025
DEFINITION  defensin-1-like [Amblyomma americanum].
ACCESSION   XP_077505888
VERSION     XP_077505888.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649762.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..72
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..72
                     /product="defensin-1-like"
                     /calculated_mol_wt=7749
     Region          <36..71
                     /region_name="Defensin_2"
                     /note="Arthropod defensin; cl03093"
                     /db_xref="CDD:470736"
     CDS             1..72
                     /gene="LOC144115389"
                     /coded_by="XM_077649762.1:70..288"
                     /db_xref="GeneID:144115389"
ORIGIN      
        1 mkvlavafif vlvgglvsta deedksqvpl vrvrrgfgcp fnqyqchshc lsigrrggyc
       61 ggsfkttctc yn
//
LOCUS       XP_077505889              74 aa            linear   INV 25-NOV-2025
DEFINITION  4 kDa defensin [Amblyomma americanum].
ACCESSION   XP_077505889
VERSION     XP_077505889.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649763.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..74
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..74
                     /product="4 kDa defensin"
                     /calculated_mol_wt=7939
     Region          <37..72
                     /region_name="Defensin_2"
                     /note="Arthropod defensin; cl03093"
                     /db_xref="CDD:470736"
     CDS             1..74
                     /gene="LOC144115390"
                     /coded_by="XM_077649763.1:72..296"
                     /db_xref="GeneID:144115390"
ORIGIN      
        1 mkvlaialif vlvaglvttv vaqddegtva hvrvrrgfgc pfnqgachrh crsigrrggy
       61 cagafkqtct cyrr
//
LOCUS       XP_077505890              74 aa            linear   INV 25-NOV-2025
DEFINITION  4 kDa defensin [Amblyomma americanum].
ACCESSION   XP_077505890
VERSION     XP_077505890.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649764.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..74
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..74
                     /product="4 kDa defensin"
                     /calculated_mol_wt=7939
     Region          <37..72
                     /region_name="Defensin_2"
                     /note="Arthropod defensin; cl03093"
                     /db_xref="CDD:470736"
     CDS             1..74
                     /gene="LOC144115391"
                     /coded_by="XM_077649764.1:72..296"
                     /db_xref="GeneID:144115391"
ORIGIN      
        1 mkvlaialif vlvaglvttv vaqddegtva hvrvrrgfgc pfnqgachrh crsigrrggy
       61 cagafkqtct cyrr
//
LOCUS       XP_077505891              74 aa            linear   INV 25-NOV-2025
DEFINITION  4 kDa defensin [Amblyomma americanum].
ACCESSION   XP_077505891
VERSION     XP_077505891.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649765.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..74
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..74
                     /product="4 kDa defensin"
                     /calculated_mol_wt=7893
     Region          <37..72
                     /region_name="Defensin_2"
                     /note="Arthropod defensin; cl03093"
                     /db_xref="CDD:470736"
     CDS             1..74
                     /gene="LOC144115392"
                     /coded_by="XM_077649765.1:63..287"
                     /db_xref="GeneID:144115392"
ORIGIN      
        1 mkvlaivlif vlvaglvgtv vaqddegava hvrvrrgfgc pfnqgachrh crsigrrggy
       61 cagafkqtct cyrr
//
LOCUS       XP_077505892              74 aa            linear   INV 25-NOV-2025
DEFINITION  4 kDa defensin [Amblyomma americanum].
ACCESSION   XP_077505892
VERSION     XP_077505892.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649766.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..74
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..74
                     /product="4 kDa defensin"
                     /calculated_mol_wt=7893
     Region          <37..72
                     /region_name="Defensin_2"
                     /note="Arthropod defensin; cl03093"
                     /db_xref="CDD:470736"
     CDS             1..74
                     /gene="LOC144115393"
                     /coded_by="XM_077649766.1:63..287"
                     /db_xref="GeneID:144115393"
ORIGIN      
        1 mkvlaivlif vlvaglvgtv vaqddegava hvrvrrgfgc pfnqgachrh crsigrrggy
       61 cagafkqtct cyrr
//
LOCUS       XP_077505893              74 aa            linear   INV 25-NOV-2025
DEFINITION  4 kDa defensin [Amblyomma americanum].
ACCESSION   XP_077505893
VERSION     XP_077505893.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649767.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..74
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..74
                     /product="4 kDa defensin"
                     /calculated_mol_wt=7939
     Region          <37..72
                     /region_name="Defensin_2"
                     /note="Arthropod defensin; cl03093"
                     /db_xref="CDD:470736"
     CDS             1..74
                     /gene="LOC144115394"
                     /coded_by="XM_077649767.1:70..294"
                     /db_xref="GeneID:144115394"
ORIGIN      
        1 mkvlaialif vlvaglvttv vaqddegtva hvrvrrgfgc pfnqgachrh crsigrrggy
       61 cagafkqtct cyrr
//
LOCUS       XP_077505894              74 aa            linear   INV 25-NOV-2025
DEFINITION  4 kDa defensin-like [Amblyomma americanum].
ACCESSION   XP_077505894
VERSION     XP_077505894.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649768.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..74
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..74
                     /product="4 kDa defensin-like"
                     /calculated_mol_wt=7797
     Region          <37..72
                     /region_name="Defensin_2"
                     /note="Arthropod defensin; cl03093"
                     /db_xref="CDD:470736"
     CDS             1..74
                     /gene="LOC144115395"
                     /coded_by="XM_077649768.1:98..322"
                     /db_xref="GeneID:144115395"
ORIGIN      
        1 mkvlavalli vlvaglvsav aaqddeseva hvrvrrgfgc plnqgachnh crsigrrggy
       61 csgiikqtct cyrk
//
LOCUS       XP_077505895              74 aa            linear   INV 25-NOV-2025
DEFINITION  4 kDa defensin-like [Amblyomma americanum].
ACCESSION   XP_077505895
VERSION     XP_077505895.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649769.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..74
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..74
                     /product="4 kDa defensin-like"
                     /calculated_mol_wt=7797
     Region          <37..72
                     /region_name="Defensin_2"
                     /note="Arthropod defensin; cl03093"
                     /db_xref="CDD:470736"
     CDS             1..74
                     /gene="LOC144115395"
                     /coded_by="XM_077649769.1:65..289"
                     /db_xref="GeneID:144115395"
ORIGIN      
        1 mkvlavalli vlvaglvsav aaqddeseva hvrvrrgfgc plnqgachnh crsigrrggy
       61 csgiikqtct cyrk
//
LOCUS       XP_077505896             153 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115396 [Amblyomma americanum].
ACCESSION   XP_077505896
VERSION     XP_077505896.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649770.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..153
                     /product="uncharacterized protein LOC144115396"
                     /calculated_mol_wt=16343
     Region          <26..73
                     /region_name="DUF4395"
                     /note="Domain of unknown function (DUF4395); pfam14340"
                     /db_xref="CDD:433882"
     CDS             1..153
                     /gene="LOC144115396"
                     /coded_by="XM_077649770.1:164..625"
                     /db_xref="GeneID:144115396"
ORIGIN      
        1 mkppvfsvpr hsrepslwgp lattviglvl glcgtvafva gfvwlqyqpv wmagvllfal
       61 slvaicsgml aykaqvdrfk hetqcqhyst pygrlhaede eacpsqrpga eksgrpgpsk
      121 appstitled sagwsgraaq pqhlhacgva vhi
//
LOCUS       XP_077505897             763 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077505897
VERSION     XP_077505897.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649771.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..763
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=85809
     Region          100..225
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          320..701
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..763
                     /gene="LOC144115397"
                     /coded_by="XM_077649771.1:511..2802"
                     /db_xref="GeneID:144115397"
ORIGIN      
        1 mpsllrrgng giahshayli tavaflllpf laqaspvenv tsvigdettt stttsaiatt
       61 eaddeakyeq alrnlvarlf anmpaslrrn llnaevstec svglfrmmrg fqnlepwalr
      121 lfdasgkypt gllqatkadl gafdeclgtv vrdnygqevs rgqycsllfh mnesralker
      181 issldvihpl ikqyteyffi devpmtrigi citndcnkrd lkalidavrp aklrievdsc
      241 vtiddelmtt gqiviiafla flgviigvat vvdiyisarv kpqasgkstw qkcllsfsav
      301 tntraifavn rdqragefdl kfihglrffs lvsivlghsy gtlsdtwsgt lnllilsehw
      361 psilvtsgfv lvdtfffisg flvclvldkq rssfrvlifa iirrlirtti plffivmciy
      421 llplmtsgpg aktffekmdq evhdhwwqwl lqirncyyvg lqsvmphvwy lsvdfqfylv
      481 avplflvlkn rrrlaigafa lislvgccva gwqvssyqia pfmvflahql stvgdtvnky
      541 ymlpfyhavc yftgcivylv adnfrqrkvs kgvevicwvv smaagvccif ikctwytgdv
      601 ptttfgrvat affdrilwsm figwialaci sgrggfvskf lswapfapls risfavylih
      661 fpfievllht sreryyyshf nqvtlffavm vwsyilayfs ylicdgptgn ldkllfgsst
      721 rprtengepa typkigngtr eavfeipriv lesdekkvin gvh
//
LOCUS       XP_077505898             389 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077505898
VERSION     XP_077505898.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649772.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..389
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=44689
     Region          <101..341
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..389
                     /gene="LOC144115399"
                     /coded_by="XM_077649772.1:668..1837"
                     /db_xref="GeneID:144115399"
ORIGIN      
        1 miiagafngv dtffflsgff lsftvtkqkg sapiifvvgm lrrlirtcvp lffiimcfyv
       61 lplfvtgpdt ktfflrfhee vvehwwhwll qiknfydmel mivlghtwfl sadfqlflvs
      121 fvtllllksr ktmtlgafvl lsllsyivva wtlasdsdvm pflilpsqrp evaiktvnny
      181 yqrpfyhamc yfsgcitfli medfrqrklt kamrlagwcv asvsmlccif mkiawykspn
      241 ptsvvgnllv afsdrflwsl flawatlvcs tgrggvfarf lscdvfvpls klsfgvylih
      301 lpfielmlha srerlfyshf tqvslffsvl vwsfllsyla ylacegptsa leklaftrlm
      361 gsgnrrrkeq qfqhneahag aeptkssyf
//
LOCUS       XP_077505899             751 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077505899
VERSION     XP_077505899.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649773.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..751
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=84042
     Region          99..225
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          312..698
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..751
                     /gene="LOC144115400"
                     /coded_by="XM_077649773.1:287..2542"
                     /db_xref="GeneID:144115400"
ORIGIN      
        1 mpslipaakg rgngpftags llttclllll vtqclcaest sdiskvhttt pamatavdti
       61 arddvyfkrv lqevvaesfi qvpaslkgkl lganvrpvck aalrrtfraf enmepwalrl
      121 fdasgkyptg lfqlnrvdlg afdecietev rgalgnelsr gqycslelsf nnfttwkrll
      181 tyfsdviqpr lfdyqdelfy idthfvrlgi clldecskqd vqalvdsvtp svitagvldc
      241 ttaepqpwdk tqirvtaflg llvvtvifat lvdlatqskr ehldknglhh glltafsaaa
      301 ntrtllhtae ktdpshhmqf lhglrffamt htvlahtygi lsdvwgnqli iligvqewrs
      361 giltsafnsv dtmfflsgff lcfavmaqgs sgslvfgvav irrlirvcip lffvimcfyv
      421 lpcivtgpns kaffdkfyve iaeewwrllf qirnfsevni qtmflhtwsl svdfqlfvvs
      481 llviltlknr kamalralga lsllgciisa wtvfdseitp fmtlpgfkas vmlktlntfy
      541 irpsyhavcy fsgcitflim ddfrrlkmsk gtqlvgwcva tscclcvvfm kapwyhrpnp
      601 asqfvkilat vsdrllwslf lawttlacst grggfvgrfl scnvfvplsk lscgvylihf
      661 plillmlhas rerlhlshft qltlffgvlt wslllaylef lmcegpiatl dksafqifir
      721 rgktaqrnlg rqtdtngapa ekpennhvhs e
//
LOCUS       XP_077505900             737 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077505900
VERSION     XP_077505900.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649774.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (DAPXPI010014853.1) annotated
            using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 228 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..737
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=82855
     Region          81..207
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          299..707
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..737
                     /gene="LOC144115401"
                     /coded_by="XM_077649774.1:579..2792"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 228 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144115401"
ORIGIN      
        1 mtlltawlli lffigssraq datgsasady aarvstteen avaaddtdyg kmlrdfvakg
       61 fasmpasftr kllaadvqpe csiallrtmr afknldpwal rlldatgkyp tgvfqvsrvd
      121 fgafdeclet evrdssgqlq slgqycnlli avknrtiter ildhlsgvih hrfldykdlv
      181 lnartpliri gicflndcse ediqalidsv ippvvkaevs ncvtaepepw nrtqaavvif
      241 ftvlaiivfg atlvdhflkp ehkakcgmpl klvtafsavt nseilfqcnd krnpdhlslq
      301 fmhgvrffsv ahivlghcye avsdswarll nmfiendhwt nliiatgfns vdtffflsgf
      361 flclvitkqk angpivflia virrlirici plffvimcfy gvprfvsgpn vkafflkfyd
      421 eiatdwwgll lqirnfsefr detllvhlwy lssdfqlfvv sllvllllks rkalslgafa
      481 vlsllgcaia mwtaadpevv pfmmfpgftk rrvlktlnly yikpfyhavc ffsgcvtlml
      541 ledfrdrkis kgvrfagwcv stscalcvmf mkvpwykhvd ptpgaakllv affdrvlwsi
      601 slawitlacs tgqgglvgrl lslnvfvpls klsfgiymih ypfiqlmlha srerqfwshf
      661 tqvtlffgvl awsvllayls fvmceaptaa ldklvfqrlt rrgrataldg aarpsgqeag
      721 kperietdlt hypverl
//
LOCUS       XP_077505901             165 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial single stranded DNA-binding protein [Amblyomma
            americanum].
ACCESSION   XP_077505901
VERSION     XP_077505901.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649775.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..165
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..165
                     /product="mitochondrial single stranded DNA-binding
                     protein"
                     /calculated_mol_wt=18268
     Region          42..157
                     /region_name="Ssb"
                     /note="Single-stranded DNA-binding protein [Replication,
                     recombination and repair]; COG0629"
                     /db_xref="CDD:440394"
     CDS             1..165
                     /gene="mtSSB"
                     /coded_by="XM_077649775.1:247..744"
                     /db_xref="GeneID:144115402"
ORIGIN      
        1 msiaagrisr wsaelllrsr pgcsllrrly ceerpatlek sinqvtllgr vgiepqlrgs
       61 danpvvvftl atnsnyryet gevqqktqwh risvfkpylr nsvhqytrkg srvlvqgrlv
      121 ygevtdakga thvtstvvad dviflsqgar tpatedesia dasyq
//
LOCUS       XP_077505902             440 aa            linear   INV 25-NOV-2025
DEFINITION  STAM-binding protein [Amblyomma americanum].
ACCESSION   XP_077505902
VERSION     XP_077505902.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649776.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..440
                     /product="STAM-binding protein"
                     /calculated_mol_wt=49699
     Region          8..118
                     /region_name="USP8_dimer"
                     /note="USP8 dimerization domain; pfam08969"
                     /db_xref="CDD:462647"
     Region          <89..>166
                     /region_name="PRK00409"
                     /note="recombination and DNA strand exchange inhibitor
                     protein; Reviewed"
                     /db_xref="CDD:234750"
     Region          267..439
                     /region_name="MPN_AMSH_like"
                     /note="Mov34/MPN/PAD-1 family; cd08066"
                     /db_xref="CDD:163697"
     Site            order(293,348,350,358,361)
                     /site_type="active"
                     /note="MPN+ (JAMM) motif [active]"
                     /db_xref="CDD:163697"
     Site            order(295..297,322..326,329..330,333,336,342..343,346,348,
                     350,358,360..361,363..364,367..368,370..371,409)
                     /site_type="other"
                     /note="Distal ubiquitin recognition interface [polypeptide
                     binding]"
                     /db_xref="CDD:163697"
     Site            order(322,350,353..354,356,358..359,409..411)
                     /site_type="active"
                     /note="Proximal ubiquitin recognition interface [active]"
                     /db_xref="CDD:163697"
     Site            order(348,350,361)
                     /site_type="other"
                     /note="Zinc-binding site [ion binding]"
                     /db_xref="CDD:163697"
     CDS             1..440
                     /gene="LOC144115403"
                     /coded_by="XM_077649776.1:306..1628"
                     /db_xref="GeneID:144115403"
ORIGIN      
        1 mpsasrglek ydphakvrnl sevgsavvid esiplkryfr sgkemlrmae vyrkegdles
       61 afilytkyit lfvekilkhp dmkgpslpew dqtkkklkei fpiaeklkse liekytqeyn
      121 iilknkerqd aeealqeerr kkdelerlmy eqqklfalqq rqqqakmavl erpdtptpps
      181 gispsdvfpm llelengiag sprpktppfp mlsdfqvpdi psraskpsvt ptapvdvvrr
      241 aipavdrstk plpllspepy flgtqglrtv vvpgglfskf lhlsrqntek nietcailag
      301 kfsrnqlnis hllvpkqsgt adscftesee evleyqdqlg ldtvgwvhth ptqtafmssv
      361 dlhthcsyql mlpeavaivc spkydenkif sltvdhglpf isgcratgfh phpnepplye
      421 ecrhvkvedk aqvivvdlrv
//
LOCUS       XP_077505903            1049 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115406 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505903
VERSION     XP_077505903.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649777.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1049
                     /product="uncharacterized protein LOC144115406 isoform X1"
                     /calculated_mol_wt=106922
     Region          798..>824
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     Region          872..907
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     CDS             1..1049
                     /gene="LOC144115406"
                     /coded_by="XM_077649777.1:133..3282"
                     /db_xref="GeneID:144115406"
ORIGIN      
        1 mlsqdsaekg aqteppaeag empsgtgsaq eesaldtdal vrgvssvelc tvsidvpesv
       61 etvvvnttns apsvsvadav lseavaaalg kdataetpag csdtsggpda aglsdiagpp
      121 naaglqdiag lpdaaglpdt aglpdtaglp natglpdtag lpnatglpdt aglpnatgqq
      181 datglpaatv vpdaislada aahssvvdse paavetvvst dalpavavss sattstvsss
      241 vqdrgcdary vrfnnrdlen lrkiqdelnv rkagglqrsp gtpivirael aspqkglsaq
      301 apqvlvvqkp spggsakkad irvihppglt vrrqlqmavp vsgassttsv ggltkvilqp
      361 agqlmsgatf qlvgavagsg gqaaqptlvl ssptraapvr atgqqlvrva apgttsvsap
      421 kgmyvvspmk lggkvamipi nvgkspqria papstssvvs gltmgpsrvt lassaantat
      481 tstarptapt ttivpvpgkv nvllkpsmgq avprsgtvaa vvqtssaaga attvttslvt
      541 svssssaavq vpgskfhyvr lvsapagttq astvlsakqp tlvpvttarp lgsavpatva
      601 sstasattlp agvrltvpia paistqqgss tgvtvstaqr vlipatatsv rpslsggqls
      661 tatlisaaga pmqnafvvvp aqyvaqgrpl lstkhlrakr lnhfdsdcpc pakqsrylqf
      721 qqgtqsspap ssgtsrvmfq tgglmgspgg fvpiaaatap ttasvstgpd hrepslatvp
      781 pakahvngts cednsrprkp cnctksqclk lycdcfange fchscncnnc fnnleheeer
      841 qkaigacler nphafrpkig kgkegdherr htkgcnckrs gclknycecy eakilcssmc
      901 kcvgcknfed sserktlmql adaaevrvqq qaaartkmsa cdlpmrpapv sdtgerlpfa
      961 fitpevveat cqcllsrade aervkkpfad versvleefg rcleaiidsa hrtkgkaaen
     1021 vekkptpasf paeraasrra ktarrvlnl
//
LOCUS       XP_077505904            1048 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115406 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505904
VERSION     XP_077505904.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649778.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1048
                     /product="uncharacterized protein LOC144115406 isoform X2"
                     /calculated_mol_wt=106851
     Region          797..>823
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     Region          871..906
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     CDS             1..1048
                     /gene="LOC144115406"
                     /coded_by="XM_077649778.1:109..3255"
                     /db_xref="GeneID:144115406"
ORIGIN      
        1 mlsqdsekga qteppaeage mpsgtgsaqe esaldtdalv rgvssvelct vsidvpesve
       61 tvvvnttnsa psvsvadavl seavaaalgk dataetpagc sdtsggpdaa glsdiagppn
      121 aaglqdiagl pdaaglpdta glpdtaglpn atglpdtagl pnatglpdta glpnatgqqd
      181 atglpaatvv pdaisladaa ahssvvdsep aavetvvstd alpavavsss attstvsssv
      241 qdrgcdaryv rfnnrdlenl rkiqdelnvr kagglqrspg tpiviraela spqkglsaqa
      301 pqvlvvqkps pggsakkadi rvihppgltv rrqlqmavpv sgassttsvg gltkvilqpa
      361 gqlmsgatfq lvgavagsgg qaaqptlvls sptraapvra tgqqlvrvaa pgttsvsapk
      421 gmyvvspmkl ggkvamipin vgkspqriap apstssvvsg ltmgpsrvtl assaantatt
      481 starptaptt tivpvpgkvn vllkpsmgqa vprsgtvaav vqtssaagaa ttvttslvts
      541 vssssaavqv pgskfhyvrl vsapagttqa stvlsakqpt lvpvttarpl gsavpatvas
      601 stasattlpa gvrltvpiap aistqqgsst gvtvstaqrv lipatatsvr pslsggqlst
      661 atlisaagap mqnafvvvpa qyvaqgrpll stkhlrakrl nhfdsdcpcp akqsrylqfq
      721 qgtqsspaps sgtsrvmfqt gglmgspggf vpiaaatapt tasvstgpdh repslatvpp
      781 akahvngtsc ednsrprkpc nctksqclkl ycdcfangef chscncnncf nnleheeerq
      841 kaigaclern phafrpkigk gkegdherrh tkgcnckrsg clknycecye akilcssmck
      901 cvgcknfeds serktlmqla daaevrvqqq aaartkmsac dlpmrpapvs dtgerlpfaf
      961 itpevveatc qcllsradea ervkkpfadv ersvleefgr cleaiidsah rtkgkaaenv
     1021 ekkptpasfp aeraasrrak tarrvlnl
//
LOCUS       XP_077505905            1044 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115406 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505905
VERSION     XP_077505905.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649779.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1044
                     /product="uncharacterized protein LOC144115406 isoform X3"
                     /calculated_mol_wt=106391
     Region          793..>819
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     Region          867..902
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     CDS             1..1044
                     /gene="LOC144115406"
                     /coded_by="XM_077649779.1:418..3552"
                     /db_xref="GeneID:144115406"
ORIGIN      
        1 maekgaqtep paeagempsg tgsaqeesal dtdalvrgvs svelctvsid vpesvetvvv
       61 nttnsapsvs vadavlseav aaalgkdata etpagcsdts ggpdaaglsd iagppnaagl
      121 qdiaglpdaa glpdtaglpd taglpnatgl pdtaglpnat glpdtaglpn atgqqdatgl
      181 paatvvpdai sladaaahss vvdsepaave tvvstdalpa vavsssatts tvsssvqdrg
      241 cdaryvrfnn rdlenlrkiq delnvrkagg lqrspgtpiv iraelaspqk glsaqapqvl
      301 vvqkpspggs akkadirvih ppgltvrrql qmavpvsgas sttsvggltk vilqpagqlm
      361 sgatfqlvga vagsggqaaq ptlvlssptr aapvratgqq lvrvaapgtt svsapkgmyv
      421 vspmklggkv amipinvgks pqriapapst ssvvsgltmg psrvtlassa antattstar
      481 ptaptttivp vpgkvnvllk psmgqavprs gtvaavvqts saagaattvt tslvtsvsss
      541 saavqvpgsk fhyvrlvsap agttqastvl sakqptlvpv ttarplgsav patvasstas
      601 attlpagvrl tvpiapaist qqgsstgvtv staqrvlipa tatsvrpsls ggqlstatli
      661 saagapmqna fvvvpaqyva qgrpllstkh lrakrlnhfd sdcpcpakqs rylqfqqgtq
      721 sspapssgts rvmfqtgglm gspggfvpia aatapttasv stgpdhreps latvppakah
      781 vngtscedns rprkpcnctk sqclklycdc fangefchsc ncnncfnnle heeerqkaig
      841 aclernphaf rpkigkgkeg dherrhtkgc nckrsgclkn ycecyeakil cssmckcvgc
      901 knfedsserk tlmqladaae vrvqqqaaar tkmsacdlpm rpapvsdtge rlpfafitpe
      961 vveatcqcll sradeaervk kpfadversv leefgrclea iidsahrtkg kaaenvekkp
     1021 tpasfpaera asrraktarr vlnl
//
LOCUS       XP_077505906            1043 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115406 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505906
VERSION     XP_077505906.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649780.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1043
                     /product="uncharacterized protein LOC144115406 isoform X4"
                     /calculated_mol_wt=106320
     Region          792..>818
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     Region          866..901
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     CDS             1..1043
                     /gene="LOC144115406"
                     /coded_by="XM_077649780.1:19..3150"
                     /db_xref="GeneID:144115406"
ORIGIN      
        1 mekgaqtepp aeagempsgt gsaqeesald tdalvrgvss velctvsidv pesvetvvvn
       61 ttnsapsvsv adavlseava aalgkdatae tpagcsdtsg gpdaaglsdi agppnaaglq
      121 diaglpdaag lpdtaglpdt aglpnatglp dtaglpnatg lpdtaglpna tgqqdatglp
      181 aatvvpdais ladaaahssv vdsepaavet vvstdalpav avsssattst vsssvqdrgc
      241 daryvrfnnr dlenlrkiqd elnvrkaggl qrspgtpivi raelaspqkg lsaqapqvlv
      301 vqkpspggsa kkadirvihp pgltvrrqlq mavpvsgass ttsvggltkv ilqpagqlms
      361 gatfqlvgav agsggqaaqp tlvlssptra apvratgqql vrvaapgtts vsapkgmyvv
      421 spmklggkva mipinvgksp qriapapsts svvsgltmgp srvtlassaa ntattstarp
      481 taptttivpv pgkvnvllkp smgqavprsg tvaavvqtss aagaattvtt slvtsvssss
      541 aavqvpgskf hyvrlvsapa gttqastvls akqptlvpvt tarplgsavp atvasstasa
      601 ttlpagvrlt vpiapaistq qgsstgvtvs taqrvlipat atsvrpslsg gqlstatlis
      661 aagapmqnaf vvvpaqyvaq grpllstkhl rakrlnhfds dcpcpakqsr ylqfqqgtqs
      721 spapssgtsr vmfqtgglmg spggfvpiaa atapttasvs tgpdhrepsl atvppakahv
      781 ngtscednsr prkpcnctks qclklycdcf angefchscn cnncfnnleh eeerqkaiga
      841 clernphafr pkigkgkegd herrhtkgcn ckrsgclkny cecyeakilc ssmckcvgck
      901 nfedsserkt lmqladaaev rvqqqaaart kmsacdlpmr papvsdtger lpfafitpev
      961 veatcqclls radeaervkk pfadversvl eefgrcleai idsahrtkgk aaenvekkpt
     1021 pasfpaeraa srraktarrv lnl
//
LOCUS       XP_077505907            1016 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115406 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077505907
VERSION     XP_077505907.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649781.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1016
                     /product="uncharacterized protein LOC144115406 isoform X5"
                     /calculated_mol_wt=103102
     Region          765..>791
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     Region          839..874
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     CDS             1..1016
                     /gene="LOC144115406"
                     /coded_by="XM_077649781.1:132..3182"
                     /db_xref="GeneID:144115406"
ORIGIN      
        1 mlsqdsaekg aqteppaeag empsgtgsaq eesaldtdal vrgvssvelc tvsidvpesv
       61 etvvvnttns apsvsvadav lseavaaalg kdataetpag csdtsggpda aglsdiagpp
      121 naaglqdiag lpdaaglpdt aglpdtaglp natglpdtag lpnatglpdt aglpnatgqq
      181 datglpaatv vpdaislada aahssvvdse paavetvvst dalpavavss sattstvsss
      241 vqdrgcdary vrfnnrdlen lrkiqdelnv rkagglqrsp gtpivirael aspqkglsaq
      301 apqvlvvqkp spggsakkad irvihppglt vrrqlqmavp vsgassttsv ggltkvilqp
      361 agqlmsgatf qlvgavagsg gqaaqptlvl ssptraapvr atgqqlvrva apgttsvsap
      421 kgmyvvspmk lggkvamipi nvgkspqria papstssvvs gltmgpsrvt lassaantat
      481 tstarptapt ttivpvpgkv nvllkpsmgq avprsgtvaa vvqtssaaga attvttslvt
      541 svssssaavq vpgskfhyvr lvsapagttq astvlsakqp tlvpvttarp lgsavpatva
      601 sstasattlp agvrltvpia paistqqgss tgvtvstaqr vlipatatsv rpslsggqls
      661 tatlisaaga pmqnafvvvp aqyvaqfqqg tqsspapssg tsrvmfqtgg lmgspggfvp
      721 iaaataptta svstgpdhre pslatvppak ahvngtsced nsrprkpcnc tksqclklyc
      781 dcfangefch scncnncfnn leheeerqka igaclernph afrpkigkgk egdherrhtk
      841 gcnckrsgcl knycecyeak ilcssmckcv gcknfedsse rktlmqlada aevrvqqqaa
      901 artkmsacdl pmrpapvsdt gerlpfafit pevveatcqc llsradeaer vkkpfadver
      961 svleefgrcl eaiidsahrt kgkaaenvek kptpasfpae raasrrakta rrvlnl
//
LOCUS       XP_077505908            1015 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115406 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077505908
VERSION     XP_077505908.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649782.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1015
                     /product="uncharacterized protein LOC144115406 isoform X6"
                     /calculated_mol_wt=103031
     Region          764..>790
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     Region          838..873
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     CDS             1..1015
                     /gene="LOC144115406"
                     /coded_by="XM_077649782.1:443..3490"
                     /db_xref="GeneID:144115406"
ORIGIN      
        1 mlsqdsekga qteppaeage mpsgtgsaqe esaldtdalv rgvssvelct vsidvpesve
       61 tvvvnttnsa psvsvadavl seavaaalgk dataetpagc sdtsggpdaa glsdiagppn
      121 aaglqdiagl pdaaglpdta glpdtaglpn atglpdtagl pnatglpdta glpnatgqqd
      181 atglpaatvv pdaisladaa ahssvvdsep aavetvvstd alpavavsss attstvsssv
      241 qdrgcdaryv rfnnrdlenl rkiqdelnvr kagglqrspg tpiviraela spqkglsaqa
      301 pqvlvvqkps pggsakkadi rvihppgltv rrqlqmavpv sgassttsvg gltkvilqpa
      361 gqlmsgatfq lvgavagsgg qaaqptlvls sptraapvra tgqqlvrvaa pgttsvsapk
      421 gmyvvspmkl ggkvamipin vgkspqriap apstssvvsg ltmgpsrvtl assaantatt
      481 starptaptt tivpvpgkvn vllkpsmgqa vprsgtvaav vqtssaagaa ttvttslvts
      541 vssssaavqv pgskfhyvrl vsapagttqa stvlsakqpt lvpvttarpl gsavpatvas
      601 stasattlpa gvrltvpiap aistqqgsst gvtvstaqrv lipatatsvr pslsggqlst
      661 atlisaagap mqnafvvvpa qyvaqfqqgt qsspapssgt srvmfqtggl mgspggfvpi
      721 aaatapttas vstgpdhrep slatvppaka hvngtscedn srprkpcnct ksqclklycd
      781 cfangefchs cncnncfnnl eheeerqkai gaclernpha frpkigkgke gdherrhtkg
      841 cnckrsgclk nycecyeaki lcssmckcvg cknfedsser ktlmqladaa evrvqqqaaa
      901 rtkmsacdlp mrpapvsdtg erlpfafitp evveatcqcl lsradeaerv kkpfadvers
      961 vleefgrcle aiidsahrtk gkaaenvekk ptpasfpaer aasrraktar rvlnl
//
LOCUS       XP_077505909            1011 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115406 isoform X7 [Amblyomma
            americanum].
ACCESSION   XP_077505909
VERSION     XP_077505909.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649783.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1011
                     /product="uncharacterized protein LOC144115406 isoform X7"
                     /calculated_mol_wt=102572
     Region          760..>786
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     Region          834..869
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     CDS             1..1011
                     /gene="LOC144115406"
                     /coded_by="XM_077649783.1:420..3455"
                     /db_xref="GeneID:144115406"
ORIGIN      
        1 maekgaqtep paeagempsg tgsaqeesal dtdalvrgvs svelctvsid vpesvetvvv
       61 nttnsapsvs vadavlseav aaalgkdata etpagcsdts ggpdaaglsd iagppnaagl
      121 qdiaglpdaa glpdtaglpd taglpnatgl pdtaglpnat glpdtaglpn atgqqdatgl
      181 paatvvpdai sladaaahss vvdsepaave tvvstdalpa vavsssatts tvsssvqdrg
      241 cdaryvrfnn rdlenlrkiq delnvrkagg lqrspgtpiv iraelaspqk glsaqapqvl
      301 vvqkpspggs akkadirvih ppgltvrrql qmavpvsgas sttsvggltk vilqpagqlm
      361 sgatfqlvga vagsggqaaq ptlvlssptr aapvratgqq lvrvaapgtt svsapkgmyv
      421 vspmklggkv amipinvgks pqriapapst ssvvsgltmg psrvtlassa antattstar
      481 ptaptttivp vpgkvnvllk psmgqavprs gtvaavvqts saagaattvt tslvtsvsss
      541 saavqvpgsk fhyvrlvsap agttqastvl sakqptlvpv ttarplgsav patvasstas
      601 attlpagvrl tvpiapaist qqgsstgvtv staqrvlipa tatsvrpsls ggqlstatli
      661 saagapmqna fvvvpaqyva qfqqgtqssp apssgtsrvm fqtgglmgsp ggfvpiaaat
      721 apttasvstg pdhrepslat vppakahvng tscednsrpr kpcnctksqc lklycdcfan
      781 gefchscncn ncfnnlehee erqkaigacl ernphafrpk igkgkegdhe rrhtkgcnck
      841 rsgclknyce cyeakilcss mckcvgcknf edsserktlm qladaaevrv qqqaaartkm
      901 sacdlpmrpa pvsdtgerlp fafitpevve atcqcllsra deaervkkpf adversvlee
      961 fgrcleaiid sahrtkgkaa envekkptpa sfpaeraasr raktarrvln l
//
LOCUS       XP_077505910            1010 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115406 isoform X8 [Amblyomma
            americanum].
ACCESSION   XP_077505910
VERSION     XP_077505910.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649784.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1010
                     /product="uncharacterized protein LOC144115406 isoform X8"
                     /calculated_mol_wt=102501
     Region          759..>785
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     Region          833..868
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     CDS             1..1010
                     /gene="LOC144115406"
                     /coded_by="XM_077649784.1:24..3056"
                     /db_xref="GeneID:144115406"
ORIGIN      
        1 mekgaqtepp aeagempsgt gsaqeesald tdalvrgvss velctvsidv pesvetvvvn
       61 ttnsapsvsv adavlseava aalgkdatae tpagcsdtsg gpdaaglsdi agppnaaglq
      121 diaglpdaag lpdtaglpdt aglpnatglp dtaglpnatg lpdtaglpna tgqqdatglp
      181 aatvvpdais ladaaahssv vdsepaavet vvstdalpav avsssattst vsssvqdrgc
      241 daryvrfnnr dlenlrkiqd elnvrkaggl qrspgtpivi raelaspqkg lsaqapqvlv
      301 vqkpspggsa kkadirvihp pgltvrrqlq mavpvsgass ttsvggltkv ilqpagqlms
      361 gatfqlvgav agsggqaaqp tlvlssptra apvratgqql vrvaapgtts vsapkgmyvv
      421 spmklggkva mipinvgksp qriapapsts svvsgltmgp srvtlassaa ntattstarp
      481 taptttivpv pgkvnvllkp smgqavprsg tvaavvqtss aagaattvtt slvtsvssss
      541 aavqvpgskf hyvrlvsapa gttqastvls akqptlvpvt tarplgsavp atvasstasa
      601 ttlpagvrlt vpiapaistq qgsstgvtvs taqrvlipat atsvrpslsg gqlstatlis
      661 aagapmqnaf vvvpaqyvaq fqqgtqsspa pssgtsrvmf qtgglmgspg gfvpiaaata
      721 pttasvstgp dhrepslatv ppakahvngt scednsrprk pcnctksqcl klycdcfang
      781 efchscncnn cfnnleheee rqkaigacle rnphafrpki gkgkegdher rhtkgcnckr
      841 sgclknycec yeakilcssm ckcvgcknfe dsserktlmq ladaaevrvq qqaaartkms
      901 acdlpmrpap vsdtgerlpf afitpevvea tcqcllsrad eaervkkpfa dversvleef
      961 grcleaiids ahrtkgkaae nvekkptpas fpaeraasrr aktarrvlnl
//
LOCUS       XP_077505911             995 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115406 isoform X9 [Amblyomma
            americanum].
ACCESSION   XP_077505911
VERSION     XP_077505911.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649785.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..995
                     /product="uncharacterized protein LOC144115406 isoform X9"
                     /calculated_mol_wt=100974
     Region          744..>770
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     Region          818..853
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     CDS             1..995
                     /gene="LOC144115406"
                     /coded_by="XM_077649785.1:148..3135"
                     /db_xref="GeneID:144115406"
ORIGIN      
        1 mpsgtgsaqe esaldtdalv rgvssvelct vsidvpesve tvvvnttnsa psvsvadavl
       61 seavaaalgk dataetpagc sdtsggpdaa glsdiagppn aaglqdiagl pdaaglpdta
      121 glpdtaglpn atglpdtagl pnatglpdta glpnatgqqd atglpaatvv pdaisladaa
      181 ahssvvdsep aavetvvstd alpavavsss attstvsssv qdrgcdaryv rfnnrdlenl
      241 rkiqdelnvr kagglqrspg tpiviraela spqkglsaqa pqvlvvqkps pggsakkadi
      301 rvihppgltv rrqlqmavpv sgassttsvg gltkvilqpa gqlmsgatfq lvgavagsgg
      361 qaaqptlvls sptraapvra tgqqlvrvaa pgttsvsapk gmyvvspmkl ggkvamipin
      421 vgkspqriap apstssvvsg ltmgpsrvtl assaantatt starptaptt tivpvpgkvn
      481 vllkpsmgqa vprsgtvaav vqtssaagaa ttvttslvts vssssaavqv pgskfhyvrl
      541 vsapagttqa stvlsakqpt lvpvttarpl gsavpatvas stasattlpa gvrltvpiap
      601 aistqqgsst gvtvstaqrv lipatatsvr pslsggqlst atlisaagap mqnafvvvpa
      661 qyvaqfqqgt qsspapssgt srvmfqtggl mgspggfvpi aaatapttas vstgpdhrep
      721 slatvppaka hvngtscedn srprkpcnct ksqclklycd cfangefchs cncnncfnnl
      781 eheeerqkai gaclernpha frpkigkgke gdherrhtkg cnckrsgclk nycecyeaki
      841 lcssmckcvg cknfedsser ktlmqladaa evrvqqqaaa rtkmsacdlp mrpapvsdtg
      901 erlpfafitp evveatcqcl lsradeaerv kkpfadvers vleefgrcle aiidsahrtk
      961 gkaaenvekk ptpasfpaer aasrraktar rvlnl
//
LOCUS       XP_077505912             983 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115406 isoform X10 [Amblyomma
            americanum].
ACCESSION   XP_077505912
VERSION     XP_077505912.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649786.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..983
                     /product="uncharacterized protein LOC144115406 isoform
                     X10"
                     /calculated_mol_wt=99507
     Region          798..>824
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     Region          872..907
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     CDS             1..983
                     /gene="LOC144115406"
                     /coded_by="XM_077649786.1:133..3084"
                     /db_xref="GeneID:144115406"
ORIGIN      
        1 mlsqdsaekg aqteppaeag empsgtgsaq eesaldtdal vrgvssvelc tvsidvpesv
       61 etvvvnttns apsvsvadav lseavaaalg kdataetpag csdtsggpda aglsdiagpp
      121 naaglqdiag lpdaaglpdt aglpdtaglp natglpdtag lpnatglpdt aglpnatgqq
      181 datglpaatv vpdaislada aahssvvdse paavetvvst dalpavavss sattstvsss
      241 vqdrgcdary vrfnnrdlen lrkiqdelnv rkagglqrsp gtpivirael aspqkglsaq
      301 apqvlvvqkp spggsakkad irvihppglt vrrqlqmavp vsgassttsv ggltkvilqp
      361 agqlmsgatf qlvgavagsg gqaaqptlvl ssptraapvr atgqqlvrva apgttsvsap
      421 kgmyvvspmk lggkvamipi nvgkspqria papstssvvs gltmgpsrvt lassaantat
      481 tstarptapt ttivpvpgkv nvllkpsmgq avprsgtvaa vvqtssaaga attvttslvt
      541 svssssaavq vpgskfhyvr lvsapagttq astvlsakqp tlvpvttarp lgsavpatva
      601 sstasattlp agvrltvpia paistqqgss tgvtvstaqr vlipatatsv rpslsggqls
      661 tatlisaaga pmqnafvvvp aqyvaqgrpl lstkhlrakr lnhfdsdcpc pakqsrylqf
      721 qqgtqsspap ssgtsrvmfq tgglmgspgg fvpiaaatap ttasvstgpd hrepslatvp
      781 pakahvngts cednsrprkp cnctksqclk lycdcfange fchscncnnc fnnleheeer
      841 qkaigacler nphafrpkig kgkegdherr htkgcnckrs gclknycecy eakilcssmc
      901 kcvgcknfed sserktlmql adaaevrvqq qaaartkmsa cdlpmrpapv sdtgesrcrt
      961 rkdatppgti qeraggpmwp adp
//
LOCUS       XP_077505913             950 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115406 isoform X11 [Amblyomma
            americanum].
ACCESSION   XP_077505913
VERSION     XP_077505913.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649787.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..950
                     /product="uncharacterized protein LOC144115406 isoform
                     X11"
                     /calculated_mol_wt=95688
     Region          765..>791
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     Region          839..874
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     CDS             1..950
                     /gene="LOC144115406"
                     /coded_by="XM_077649787.1:133..2985"
                     /db_xref="GeneID:144115406"
ORIGIN      
        1 mlsqdsaekg aqteppaeag empsgtgsaq eesaldtdal vrgvssvelc tvsidvpesv
       61 etvvvnttns apsvsvadav lseavaaalg kdataetpag csdtsggpda aglsdiagpp
      121 naaglqdiag lpdaaglpdt aglpdtaglp natglpdtag lpnatglpdt aglpnatgqq
      181 datglpaatv vpdaislada aahssvvdse paavetvvst dalpavavss sattstvsss
      241 vqdrgcdary vrfnnrdlen lrkiqdelnv rkagglqrsp gtpivirael aspqkglsaq
      301 apqvlvvqkp spggsakkad irvihppglt vrrqlqmavp vsgassttsv ggltkvilqp
      361 agqlmsgatf qlvgavagsg gqaaqptlvl ssptraapvr atgqqlvrva apgttsvsap
      421 kgmyvvspmk lggkvamipi nvgkspqria papstssvvs gltmgpsrvt lassaantat
      481 tstarptapt ttivpvpgkv nvllkpsmgq avprsgtvaa vvqtssaaga attvttslvt
      541 svssssaavq vpgskfhyvr lvsapagttq astvlsakqp tlvpvttarp lgsavpatva
      601 sstasattlp agvrltvpia paistqqgss tgvtvstaqr vlipatatsv rpslsggqls
      661 tatlisaaga pmqnafvvvp aqyvaqfqqg tqsspapssg tsrvmfqtgg lmgspggfvp
      721 iaaataptta svstgpdhre pslatvppak ahvngtsced nsrprkpcnc tksqclklyc
      781 dcfangefch scncnncfnn leheeerqka igaclernph afrpkigkgk egdherrhtk
      841 gcnckrsgcl knycecyeak ilcssmckcv gcknfedsse rktlmqlada aevrvqqqaa
      901 artkmsacdl pmrpapvsdt gesrcrtrkd atppgtiqer aggpmwpadp
//
LOCUS       XP_077505914             913 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115406 isoform X12 [Amblyomma
            americanum].
ACCESSION   XP_077505914
VERSION     XP_077505914.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649788.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..913
                     /product="uncharacterized protein LOC144115406 isoform
                     X12"
                     /calculated_mol_wt=91721
     Region          798..>824
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     Region          872..>891
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     CDS             1..913
                     /gene="LOC144115406"
                     /coded_by="XM_077649788.1:133..2874"
                     /db_xref="GeneID:144115406"
ORIGIN      
        1 mlsqdsaekg aqteppaeag empsgtgsaq eesaldtdal vrgvssvelc tvsidvpesv
       61 etvvvnttns apsvsvadav lseavaaalg kdataetpag csdtsggpda aglsdiagpp
      121 naaglqdiag lpdaaglpdt aglpdtaglp natglpdtag lpnatglpdt aglpnatgqq
      181 datglpaatv vpdaislada aahssvvdse paavetvvst dalpavavss sattstvsss
      241 vqdrgcdary vrfnnrdlen lrkiqdelnv rkagglqrsp gtpivirael aspqkglsaq
      301 apqvlvvqkp spggsakkad irvihppglt vrrqlqmavp vsgassttsv ggltkvilqp
      361 agqlmsgatf qlvgavagsg gqaaqptlvl ssptraapvr atgqqlvrva apgttsvsap
      421 kgmyvvspmk lggkvamipi nvgkspqria papstssvvs gltmgpsrvt lassaantat
      481 tstarptapt ttivpvpgkv nvllkpsmgq avprsgtvaa vvqtssaaga attvttslvt
      541 svssssaavq vpgskfhyvr lvsapagttq astvlsakqp tlvpvttarp lgsavpatva
      601 sstasattlp agvrltvpia paistqqgss tgvtvstaqr vlipatatsv rpslsggqls
      661 tatlisaaga pmqnafvvvp aqyvaqgrpl lstkhlrakr lnhfdsdcpc pakqsrylqf
      721 qqgtqsspap ssgtsrvmfq tgglmgspgg fvpiaaatap ttasvstgpd hrepslatvp
      781 pakahvngts cednsrprkp cnctksqclk lycdcfange fchscncnnc fnnleheeer
      841 qkaigacler nphafrpkig kgkegdherr htkgcnckrs gclknycecy evaiclhhsg
      901 sggshlpvpa itc
//
LOCUS       XP_077505915             880 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115406 isoform X13 [Amblyomma
            americanum].
ACCESSION   XP_077505915
VERSION     XP_077505915.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649789.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..880
                     /product="uncharacterized protein LOC144115406 isoform
                     X13"
                     /calculated_mol_wt=87902
     Region          765..>791
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     Region          839..>858
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     CDS             1..880
                     /gene="LOC144115406"
                     /coded_by="XM_077649789.1:133..2775"
                     /db_xref="GeneID:144115406"
ORIGIN      
        1 mlsqdsaekg aqteppaeag empsgtgsaq eesaldtdal vrgvssvelc tvsidvpesv
       61 etvvvnttns apsvsvadav lseavaaalg kdataetpag csdtsggpda aglsdiagpp
      121 naaglqdiag lpdaaglpdt aglpdtaglp natglpdtag lpnatglpdt aglpnatgqq
      181 datglpaatv vpdaislada aahssvvdse paavetvvst dalpavavss sattstvsss
      241 vqdrgcdary vrfnnrdlen lrkiqdelnv rkagglqrsp gtpivirael aspqkglsaq
      301 apqvlvvqkp spggsakkad irvihppglt vrrqlqmavp vsgassttsv ggltkvilqp
      361 agqlmsgatf qlvgavagsg gqaaqptlvl ssptraapvr atgqqlvrva apgttsvsap
      421 kgmyvvspmk lggkvamipi nvgkspqria papstssvvs gltmgpsrvt lassaantat
      481 tstarptapt ttivpvpgkv nvllkpsmgq avprsgtvaa vvqtssaaga attvttslvt
      541 svssssaavq vpgskfhyvr lvsapagttq astvlsakqp tlvpvttarp lgsavpatva
      601 sstasattlp agvrltvpia paistqqgss tgvtvstaqr vlipatatsv rpslsggqls
      661 tatlisaaga pmqnafvvvp aqyvaqfqqg tqsspapssg tsrvmfqtgg lmgspggfvp
      721 iaaataptta svstgpdhre pslatvppak ahvngtsced nsrprkpcnc tksqclklyc
      781 dcfangefch scncnncfnn leheeerqka igaclernph afrpkigkgk egdherrhtk
      841 gcnckrsgcl knycecyeva iclhhsgsgg shlpvpaitc
//
LOCUS       XP_077505916             356 aa            linear   INV 25-NOV-2025
DEFINITION  heterogeneous nuclear ribonucleoprotein A/B-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077505916
VERSION     XP_077505916.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649790.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..356
                     /product="heterogeneous nuclear ribonucleoprotein A/B-like
                     isoform X1"
                     /calculated_mol_wt=37645
     Region          69..140
                     /region_name="RRM1_hnRNPA_hnRNPD_like"
                     /note="RNA recognition motif 1 (RRM1) found in
                     heterogeneous nuclear ribonucleoprotein hnRNP A and hnRNP
                     D subfamilies and similar proteins; cd12325"
                     /db_xref="CDD:409763"
     Site            order(70,72..75,97,99,108..110,112,135,138,140)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:409763"
     Region          150..224
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..356
                     /gene="LOC144115408"
                     /coded_by="XM_077649790.1:65..1135"
                     /db_xref="GeneID:144115408"
ORIGIN      
        1 madgdqqgny gegdfsadgq ygeyndgqft dgqyqqegeg ngqtneaaqn gsdgktnede
       61 rndlfsrklf vggiswdtdn kdlreyfskf gvvvdvnikt dpttgksrgf gfvtfnakda
      121 ieavlkatph tvkgkqidpk pakarpgikk ifvgglesdm peadikayfe kfgpvenvel
      181 pfdkaknqrr qfafvtfere dsvdlvcrep kqkignkecd ikkatpkpda rgmrggwggs
      241 fgaggrggrg grggrgrggg yqnqgwgnqg yggggygggg ygqgygggyg qgygggygny
      301 dygsgygggy dysgwnyggg qggygsgygq qqsnynktrr gggggagggg yhpysr
//
LOCUS       XP_077505917             350 aa            linear   INV 25-NOV-2025
DEFINITION  heterogeneous nuclear ribonucleoprotein D0-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077505917
VERSION     XP_077505917.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649791.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..350
                     /product="heterogeneous nuclear ribonucleoprotein D0-like
                     isoform X2"
                     /calculated_mol_wt=36912
     Region          63..134
                     /region_name="RRM1_hnRNPA_hnRNPD_like"
                     /note="RNA recognition motif 1 (RRM1) found in
                     heterogeneous nuclear ribonucleoprotein hnRNP A and hnRNP
                     D subfamilies and similar proteins; cd12325"
                     /db_xref="CDD:409763"
     Site            order(64,66..69,91,93,102..104,106,129,132,134)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:409763"
     Region          144..218
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..350
                     /gene="LOC144115408"
                     /coded_by="XM_077649791.1:63..1115"
                     /db_xref="GeneID:144115408"
ORIGIN      
        1 madgdqqgny gegdfsadgq ygeyndgqft dgqyqqegeg ngqtneaaqn gsdgktnede
       61 rklfvggisw dtdnkdlrey fskfgvvvdv niktdpttgk srgfgfvtfn akdaieavlk
      121 atphtvkgkq idpkpakarp gikkifvggl esdmpeadik ayfekfgpve nvelpfdkak
      181 nqrrqfafvt feredsvdlv crepkqkign kecdikkatp kpdargmrgg wggsfgaggr
      241 ggrggrggrg rgggyqnqgw gnqgyggggy ggggygqgyg ggygqgyggg ygnydygsgy
      301 gggydysgwn ygggqggygs gygqqqsnyn ktrrggggga ggggyhpysr
//
LOCUS       XP_077505918             325 aa            linear   INV 25-NOV-2025
DEFINITION  heterogeneous nuclear ribonucleoprotein A/B-like isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077505918
VERSION     XP_077505918.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649792.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..325
                     /product="heterogeneous nuclear ribonucleoprotein A/B-like
                     isoform X3"
                     /calculated_mol_wt=34507
     Region          69..140
                     /region_name="RRM1_hnRNPA_hnRNPD_like"
                     /note="RNA recognition motif 1 (RRM1) found in
                     heterogeneous nuclear ribonucleoprotein hnRNP A and hnRNP
                     D subfamilies and similar proteins; cd12325"
                     /db_xref="CDD:409763"
     Site            order(70,72..75,97,99,108..110,112,135,138,140)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:409763"
     Region          150..224
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..325
                     /gene="LOC144115408"
                     /coded_by="XM_077649792.1:66..1043"
                     /db_xref="GeneID:144115408"
ORIGIN      
        1 madgdqqgny gegdfsadgq ygeyndgqft dgqyqqegeg ngqtneaaqn gsdgktnede
       61 rndlfsrklf vggiswdtdn kdlreyfskf gvvvdvnikt dpttgksrgf gfvtfnakda
      121 ieavlkatph tvkgkqidpk pakarpgikk ifvgglesdm peadikayfe kfgpvenvel
      181 pfdkaknqrr qfafvtfere dsvdlvcrep kqkignkecd ikkatpkpda rgmrggwggs
      241 fgaggrggrg grggrgrggg yqnqgwgnqg yggggygggg ygqgygggyg qgygggygqq
      301 qsnynktrrg ggggaggggy hpysr
//
LOCUS       XP_077505919             319 aa            linear   INV 25-NOV-2025
DEFINITION  heterogeneous nuclear ribonucleoprotein A/B-like isoform X4
            [Amblyomma americanum].
ACCESSION   XP_077505919
VERSION     XP_077505919.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649793.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..319
                     /product="heterogeneous nuclear ribonucleoprotein A/B-like
                     isoform X4"
                     /calculated_mol_wt=33774
     Region          63..134
                     /region_name="RRM1_hnRNPA_hnRNPD_like"
                     /note="RNA recognition motif 1 (RRM1) found in
                     heterogeneous nuclear ribonucleoprotein hnRNP A and hnRNP
                     D subfamilies and similar proteins; cd12325"
                     /db_xref="CDD:409763"
     Site            order(64,66..69,91,93,102..104,106,129,132,134)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:409763"
     Region          144..218
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..319
                     /gene="LOC144115408"
                     /coded_by="XM_077649793.1:64..1023"
                     /db_xref="GeneID:144115408"
ORIGIN      
        1 madgdqqgny gegdfsadgq ygeyndgqft dgqyqqegeg ngqtneaaqn gsdgktnede
       61 rklfvggisw dtdnkdlrey fskfgvvvdv niktdpttgk srgfgfvtfn akdaieavlk
      121 atphtvkgkq idpkpakarp gikkifvggl esdmpeadik ayfekfgpve nvelpfdkak
      181 nqrrqfafvt feredsvdlv crepkqkign kecdikkatp kpdargmrgg wggsfgaggr
      241 ggrggrggrg rgggyqnqgw gnqgyggggy ggggygqgyg ggygqgyggg ygqqqsnynk
      301 trrgggggag gggyhpysr
//
LOCUS       XP_077505921             152 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase RNF181-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505921
VERSION     XP_077505921.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649795.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..152
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..152
                     /product="E3 ubiquitin-protein ligase RNF181-like isoform
                     X1"
                     /calculated_mol_wt=17303
     Region          74..119
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(75,78,93,95,98,101,112,115)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          <122..>145
                     /region_name="ZIP_TSC22D-like"
                     /note="leucine zipper found in the TSC22 domain leucine
                     zipper transcription factors, c-Myc-binding protein, and
                     similar proteins; cl40461"
                     /db_xref="CDD:424092"
     CDS             1..152
                     /gene="LOC144115409"
                     /coded_by="XM_077649795.1:164..622"
                     /db_xref="GeneID:144115409"
ORIGIN      
        1 masyfdehgc eplgpgeqpn hslhlarlli dggfrasfdt eyhrmfpdep hkppaskqav
       61 qslkrapvee adkscsiclk kygheevvte lpcchffhdd cilpwlqvvn tcpmcrhelp
      121 tddpnyealk aekermkqrq qaleelhnsm fg
//
LOCUS       XP_077505922             139 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase RNF181-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505922
VERSION     XP_077505922.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649796.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..139
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..139
                     /product="E3 ubiquitin-protein ligase RNF181-like isoform
                     X2"
                     /calculated_mol_wt=15583
     Region          74..119
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(75,78,93,95,98,101,112,115)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     CDS             1..139
                     /gene="LOC144115409"
                     /coded_by="XM_077649796.1:170..589"
                     /db_xref="GeneID:144115409"
ORIGIN      
        1 masyfdehgc eplgpgeqpn hslhlarlli dggfrasfdt eyhrmfpdep hkppaskqav
       61 qslkrapvee adkscsiclk kygheevvte lpcchffhdd cilpwlqvvn tcpmcrhelp
      121 tddpnyealk aekgqpskp
//
LOCUS       XP_077505923             110 aa            linear   INV 25-NOV-2025
DEFINITION  protein lin-54 homolog isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505923
VERSION     XP_077505923.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649797.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..110
                     /product="protein lin-54 homolog isoform X1"
                     /calculated_mol_wt=11710
     CDS             1..110
                     /gene="LOC144115410"
                     /coded_by="XM_077649797.1:109..441"
                     /db_xref="GeneID:144115410"
ORIGIN      
        1 matcarqstk slgvflpllt qwksdqamch cssmasaaid gtwiatqaki lcssmckcvg
       61 cknfedsser ktlmqladaa evrvqqqaaa rtkmsacdlp mrpapvsdsc
//
LOCUS       XP_077505924             110 aa            linear   INV 25-NOV-2025
DEFINITION  protein lin-54 homolog isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505924
VERSION     XP_077505924.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649798.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..110
                     /product="protein lin-54 homolog isoform X2"
                     /calculated_mol_wt=12115
     CDS             1..110
                     /gene="LOC144115410"
                     /coded_by="XM_077649798.1:145..477"
                     /db_xref="GeneID:144115410"
ORIGIN      
        1 mctcvdtpsp aafhfisctf hsqrryrqch lkstkrlksc lvlgkheaki lcssmckcvg
       61 cknfedsser ktlmqladaa evrvqqqaaa rtkmsacdlp mrpapvsdsc
//
LOCUS       XP_077505925              97 aa            linear   INV 25-NOV-2025
DEFINITION  protein lin-54 homolog isoform X3 [Amblyomma americanum].
ACCESSION   XP_077505925
VERSION     XP_077505925.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649799.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..97
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..97
                     /product="protein lin-54 homolog isoform X3"
                     /calculated_mol_wt=10423
     CDS             1..97
                     /gene="LOC144115410"
                     /coded_by="XM_077649799.1:109..402"
                     /db_xref="GeneID:144115410"
ORIGIN      
        1 matcarqstk slgvflpllt qwksdqamch cssmakilcs smckcvgckn fedsserktl
       61 mqladaaevr vqqqaaartk msacdlpmrp apvsdsc
//
LOCUS       XP_077505926              88 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115410 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505926
VERSION     XP_077505926.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649800.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..88
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..88
                     /product="uncharacterized protein LOC144115410 isoform X4"
                     /calculated_mol_wt=9792
     CDS             1..88
                     /gene="LOC144115410"
                     /coded_by="XM_077649800.1:145..411"
                     /db_xref="GeneID:144115410"
ORIGIN      
        1 mctcvdtpsp aafhfisctf hsqrryrqch lkstkrlksc lvlgkheipg slptppdtve
       61 krssnvpllf ygqdpvqqhv qvcglqel
//
LOCUS       XP_077505927              83 aa            linear   INV 25-NOV-2025
DEFINITION  protein lin-54 homolog isoform X5 [Amblyomma americanum].
ACCESSION   XP_077505927
VERSION     XP_077505927.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649801.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..83
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..83
                     /product="protein lin-54 homolog isoform X5"
                     /calculated_mol_wt=8746
     CDS             1..83
                     /gene="LOC144115410"
                     /coded_by="XM_077649801.1:1251..1502"
                     /db_xref="GeneID:144115410"
ORIGIN      
        1 mchcssmasa aidgtwiatq akilcssmck cvgcknfeds serktlmqla daaevrvqqq
       61 aaartkmsac dlpmrpapvs dsc
//
LOCUS       XP_077505928              83 aa            linear   INV 25-NOV-2025
DEFINITION  protein lin-54 homolog isoform X5 [Amblyomma americanum].
ACCESSION   XP_077505928
VERSION     XP_077505928.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649802.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..83
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..83
                     /product="protein lin-54 homolog isoform X5"
                     /calculated_mol_wt=8746
     CDS             1..83
                     /gene="LOC144115410"
                     /coded_by="XM_077649802.1:338..589"
                     /db_xref="GeneID:144115410"
ORIGIN      
        1 mchcssmasa aidgtwiatq akilcssmck cvgcknfeds serktlmqla daaevrvqqq
       61 aaartkmsac dlpmrpapvs dsc
//
LOCUS       XP_077505929             588 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: calcium/calmodulin-dependent protein kinase II
            [Amblyomma americanum].
ACCESSION   XP_077505929
VERSION     XP_077505929.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649803.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..588
                     /product="LOW QUALITY PROTEIN:
                     calcium/calmodulin-dependent protein kinase II"
                     /calculated_mol_wt=65391
     Region          28..319
                     /region_name="STKc_CaMKII"
                     /note="Catalytic domain of the Serine/Threonine kinase,
                     Calcium/calmodulin-dependent protein kinase Type II;
                     cd14086"
                     /db_xref="CDD:270988"
     Site            order(36..40,44,57,59,90,106..109,113,115,151..152,154,
                     156..157,159,172..173,176,191..195,197)
                     /site_type="active"
                     /db_xref="CDD:270988"
     Site            order(36..39,44,57,59,90,106..109,113,156..157,159,
                     172..173)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270988"
     Site            order(40,113,115,151..152,154,156,176,191..195,197)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270988"
     Site            order(172..182,185..195)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270988"
     Site            order(310,312..319)
                     /site_type="other"
                     /note="CaM binding site [polypeptide binding]"
                     /db_xref="CDD:270988"
     Region          467..576
                     /region_name="NTF2_like"
                     /note="Nuclear transport factor 2 (NTF2-like) superfamily.
                     This family includes members of the NTF2 family,
                     Delta-5-3-ketosteroid isomerases, Scytalone Dehydratases,
                     and the beta subunit of Ring hydroxylating dioxygenases.
                     This family is a classic example of...; cl09109"
                     /db_xref="CDD:471850"
     CDS             1..588
                     /gene="CaMKII"
                     /coded_by="XM_077649803.1:80..1846"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:144115411"
ORIGIN      
        1 mrfretfhlp ssslinmaap tpttrfsdny elkeelgkga fsivrrcvqk stglefaaki
       61 intkklssrd fqklereari crklnhpniv rlhdsiqeeg yhylifdlvt ggelfediva
      121 reyyseadas hciqqilesv nhchqnnvvh rdlkpenlll askakgaavk ladfglaiev
      181 qgeqqawygf agtpgylspe vlkkdpygkp vdiwacgvil yillvgyppf wdedqhrlya
      241 qikagaydyp spewdtvtpe aknlinsmlt vnpakritas ealkhpwicq rervastlhr
      301 qetvdclkkf narrklkgai lttmlatrnf sshvttpntg rsimtkkgeg sqvkestdss
      361 ttiedddvkd nvhaapersk tviqvdqvks pcfdskgnpk stqdiaqvan lasavasptc
      421 svptitflsa sdgdmgsgna qtllcsgnfq gvkcairnnn gsahdprkqe ivklteqlie
      481 sinsgdyety tklcdphlta fepealgagk syksqnttii nptvhllgdd aaciayirlm
      541 qyidragipr trqteetrvw hrkdtkwqnv hfhrsgnsag mhpdpsak
//
LOCUS       XP_077505930             428 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115412 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505930
VERSION     XP_077505930.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649804.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..428
                     /product="uncharacterized protein LOC144115412 isoform X1"
                     /calculated_mol_wt=47475
     CDS             1..428
                     /gene="LOC144115412"
                     /coded_by="XM_077649804.1:29..1315"
                     /db_xref="GeneID:144115412"
ORIGIN      
        1 mwcvcgmrlr clsgrktfae ynaaaamnss vsansrwwcv pstwprtdfd attattatta
       61 saptaglsis aqlcktlssr vmhfhlccqa lsldvpktpg pvqvkkalfn aktsqkadks
      121 qkvvpiksvf ihrlllqrhv aappkknipv eepslegtke vvvaelqlla sdtlsgvtyn
      181 tervmcflkv tfqdhcqhyv tlcqviswek issrqlcaea qlrfestvdk levrlkkaaq
      241 vfseqqgqdt slvqisrhlq gsldhflite talptavqvt mphvytyknq swiyggtqae
      301 vialknknie wafivcllvy ylinlrfrnr ilvcmvresv wyggmagcsr mvavfvqril
      361 slgastgpgk qpdcdlaafl asfrhshhip kslkresvtp qvsdgarnks rdergaggar
      421 rtykwsgs
//
LOCUS       XP_077505931             401 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115412 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505931
VERSION     XP_077505931.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649805.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..401
                     /product="uncharacterized protein LOC144115412 isoform X2"
                     /calculated_mol_wt=44463
     CDS             1..401
                     /gene="LOC144115412"
                     /coded_by="XM_077649805.1:29..1234"
                     /db_xref="GeneID:144115412"
ORIGIN      
        1 mwcvcgmrlr clsgrktfae ynaaaamnss vsansrwwcv pstwprtdfd attattatta
       61 saptaglsis tpgpvqvkka lfnaktsqka dksqkvvpik svfihrlllq rhvaappkkn
      121 ipveepsleg tkevvvaelq llasdtlsgv tyntervmcf lkvtfqdhcq hyvtlcqvis
      181 wekissrqlc aeaqlrfest vdklevrlkk aaqvfseqqg qdtslvqisr hlqgsldhfl
      241 itetalptav qvtmphvyty knqswiyggt qaevialknk niewafivcl lvyylinlrf
      301 rnrilvcmvr esvwyggmag csrmvavfvq rilslgastg pgkqpdcdla aflasfrhsh
      361 hipkslkres vtpqvsdgar nksrdergag garrtykwsg s
//
LOCUS       XP_077505932             875 aa            linear   INV 25-NOV-2025
DEFINITION  endothelin-converting enzyme 1-like [Amblyomma americanum].
ACCESSION   XP_077505932
VERSION     XP_077505932.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649806.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..875
                     /product="endothelin-converting enzyme 1-like"
                     /calculated_mol_wt=97356
     Region          264..872
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     CDS             1..875
                     /gene="LOC144115413"
                     /coded_by="XM_077649806.1:164..2791"
                     /db_xref="GeneID:144115413"
ORIGIN      
        1 maaqgsreqs qysrrssrsq rrgrlprala aedrlseara srelpppppp pppqqhpwad
       61 srqsagrsta sslisrlgkh ssrppssmld eeaamrrsat svagaasgmt pvrltryygp
      121 prlgrerqee eaaefgphvd gsrtwevgge mpgrrldrap laadaggsrv srrsasrqar
      181 tvaecvapas tpprparskd agdrphrtle mtfamgafvc vatlvvafvv rawmpyears
      241 dpfcrsdacl ahvrlieari drgvdpcvdf dayacsrwkp asefyghasa ltnvlldnwf
      301 knflstlskg sqlipttlkl qamlnacmts tngpqgaplr mlkafmrerk ipwpdkplpd
      361 atplgvlldf alnwavdfwf eirmlrnsrg tmgivlapgn ffmlwgkplr nivpsdryfe
      421 ywngfyaafa gsggqpksaa seirriadie edvvmtlssa ferqsklplr fmlqeiekyt
      481 sripvsdwte afetnvhllp dhvgvsdial leavnklfak ytrsellhhl swffvqvfap
      541 fgdvqlnvad inderaakqr qvfcvtqaea ayrvlvsaly lqplleanqk ravedhferv
      601 rrvtvqkian vswsdnlsky matvklqqlq tflwppsdml tasglsylya dfasnastvi
      661 efwrdahehv ralrdksfyg tpmtklpaas spplvrydyl rnavvlsvaa ltaplfalha
      721 tpamlygglm fsyalevvra ldrtgmkvdp hgdvaakwas snwekgvtrk hsclaptfas
      781 pfpeipglev aheafeials saathgkawr ttdafseqqv ffisacftlc rlnrtaaflr
      841 gdcnkafsns rqfadafrcv pgspmnplrk ctffd
//
LOCUS       XP_077505933             211 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115414 [Amblyomma americanum].
ACCESSION   XP_077505933
VERSION     XP_077505933.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649807.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..211
                     /product="uncharacterized protein LOC144115414"
                     /calculated_mol_wt=23642
     Region          7..91
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     Region          162..196
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..211
                     /gene="LOC144115414"
                     /coded_by="XM_077649807.1:86..721"
                     /db_xref="GeneID:144115414"
ORIGIN      
        1 masfneklvq evkkhtqlwd qhsklhkeag yreaawmeia aalavtveec qtrwrtlrdt
       61 ylkrkkrrra gangqwnvle rdlgflddfl rprtrrstln aaapadvevk qeqpgedaem
      121 reaavaaaaa ppqpwplamr paavmphrle aggaaaqals sdpeelfcls laarlmrllp
      181 rerhmarmkm lqtlhdlesg enigefalgd p
//
LOCUS       XP_077505934             211 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115415 [Amblyomma americanum].
ACCESSION   XP_077505934
VERSION     XP_077505934.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649808.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..211
                     /product="uncharacterized protein LOC144115415"
                     /calculated_mol_wt=23447
     Region          7..91
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     Region          162..196
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..211
                     /gene="LOC144115415"
                     /coded_by="XM_077649808.1:86..721"
                     /db_xref="GeneID:144115415"
ORIGIN      
        1 masfneklvq eakkhtqlwd qhsklhkeag yreaawmeia aalavtveec qtrlrtlrgt
       61 ylkrkkrrra gangqwnvle rdlgflddfl rprtrrstln aaapadvevk qeqpgedaem
      121 reaavaaaaa ppqpwplamr paavmphrla aggaaaqals sdpeelfcli laarlkrllp
      181 rkrhmarmkm lqtlhdlesg enigefalgd p
//
LOCUS       XP_077505935             184 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115416 [Amblyomma americanum].
ACCESSION   XP_077505935
VERSION     XP_077505935.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649809.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..184
                     /product="uncharacterized protein LOC144115416"
                     /calculated_mol_wt=20383
     Region          <14..58
                     /region_name="GT1"
                     /note="myb-like, SANT family; cl23759"
                     /db_xref="CDD:474047"
     Region          135..169
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..184
                     /gene="LOC144115416"
                     /coded_by="XM_077649809.1:79..633"
                     /db_xref="GeneID:144115416"
ORIGIN      
        1 mvvltanrvf pgaveecqtr wrtlrdtylk rkkrrradat gqwsvldrel gflddflrpr
       61 trrstlngaa paassaevev kreqpdedae vresaavvaa aaapaqpwal amrptrvmph
      121 rlaaggaaaq alfsdpeelf clslaarlkr llprerymar mkmlqtlhdl esgenigefa
      181 lgdp
//
LOCUS       XP_077505936             103 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115417 [Amblyomma americanum].
ACCESSION   XP_077505936
VERSION     XP_077505936.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649810.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..103
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..103
                     /product="uncharacterized protein LOC144115417"
                     /calculated_mol_wt=11437
     CDS             1..103
                     /gene="LOC144115417"
                     /coded_by="XM_077649810.1:86..397"
                     /db_xref="GeneID:144115417"
ORIGIN      
        1 mvvctvdmae drlrcdycdy cdhslsthsr ivdlgtimqd teqqsnafss vlsssqpgcs
       61 kdtwpctsee spvqcedite grqepescsh qvssyidycf sdm
//
LOCUS       XP_077505937             416 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115418 [Amblyomma americanum].
ACCESSION   XP_077505937
VERSION     XP_077505937.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649811.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..416
                     /product="uncharacterized protein LOC144115418"
                     /calculated_mol_wt=45100
     Region          367..401
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..416
                     /gene="LOC144115418"
                     /coded_by="XM_077649811.1:98..1348"
                     /db_xref="GeneID:144115418"
ORIGIN      
        1 maaqgsreqs qysrrssrsq rrgrlprala aedrlseara srelpppppp pppqqhpwad
       61 srqsagrsta sslisrlgkh ssrppssmld eeaamrrsat svagaasgmt pvrltryygp
      121 prlgrerqee eaaefgphvd gsrtwevgge mpgrrldrap laadaggsrv srrsasrqar
      181 tvaecvapas tpprparskd agdrphrtle mtfamgafvc vatlvvafvv rawmpyears
      241 dpfcrsdacl ahvrlieari drgvdpcvdf dayacsrwkp asefyghasa ltnvlldnwr
      301 rstlnaaapa dvevnqeqpg edaemreaav aaaaappqpw plamrpaavm phrlaaggaa
      361 aqalssdpee lfclslaarl krllprerhm armkmlqtlh dlesgenige falgdp
//
LOCUS       XP_077505938             352 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115419 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505938
VERSION     XP_077505938.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649812.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..352
                     /product="uncharacterized protein LOC144115419 isoform X1"
                     /calculated_mol_wt=39156
     CDS             1..352
                     /gene="LOC144115419"
                     /coded_by="XM_077649812.1:54..1112"
                     /db_xref="GeneID:144115419"
ORIGIN      
        1 mnssvsansr wwcvpstwpr tdfdattatt attasaptag lsisaqlckt lssrvmhfhl
       61 ccqalsldvp ktpgpvqvkk aqfnaktsqk adksqkvvpi ktpglvqvkk aqfnaktsqk
      121 adksqkvvpi kllasdtlsg vtyntervmc llkvtfqdhc qhyvtlcevi swekissrql
      181 caeaqlrfes tvdelevrlk kaaqvfseqq gqdtslvqis rhlqgsldhf litetalpta
      241 vqiitphvyt yknqswiygg tqaevialkn kniewavivc llvyylknls ymhaflqtli
      301 lvqkllrpqv qippgvisnr lkcflarskk kncpintvtk nhpgtndrrs ni
//
LOCUS       XP_077505939             278 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115419 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505939
VERSION     XP_077505939.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649813.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..278
                     /product="uncharacterized protein LOC144115419 isoform X2"
                     /calculated_mol_wt=31679
     CDS             1..278
                     /gene="LOC144115419"
                     /coded_by="XM_077649813.1:657..1493"
                     /db_xref="GeneID:144115419"
ORIGIN      
        1 mrrhhrrqtr arklfpsrvs syidycfsdv aappkknipv eepslegtke vvvaelqlla
       61 sdtlsgvtyn tervmcllkv tfqdhcqhyv tlceviswek issrqlcaea qlrfestvde
      121 levrlkkaaq vfseqqgqdt slvqisrhlq gsldhflite talptavqii tphvytyknq
      181 swiyggtqae vialknknie wavivcllvy ylknlsymha flqtlilvqk llrpqvqipp
      241 gvisnrlkcf larskkkncp intvtknhpg tndrrsni
//
LOCUS       XP_077505940             257 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115419 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505940
VERSION     XP_077505940.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649814.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..257
                     /product="uncharacterized protein LOC144115419 isoform X3"
                     /calculated_mol_wt=29198
     CDS             1..257
                     /gene="LOC144115419"
                     /coded_by="XM_077649814.1:304..1077"
                     /db_xref="GeneID:144115419"
ORIGIN      
        1 mrrhhrrqtr arklfpssfs agctrsdfyt sslkpmhrst knrvssyidy cfsdvaappk
       61 knipveepsl egtkevvvae lqllasdtls gvtyntervm cllkvtfqdh cqhyvtlcev
      121 iswekissre lcaeaqlrfe stvdelevrl kkaaqvfseq qgqdtslvqi srhlqgsldh
      181 flitetalpt avqvitphvy tyknqswiyg gtqaevialk nkniewaviv cllvyylknl
      241 symhaffads yfgaktp
//
LOCUS       XP_077505941             257 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115419 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077505941
VERSION     XP_077505941.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649815.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..257
                     /product="uncharacterized protein LOC144115419 isoform X3"
                     /calculated_mol_wt=29198
     CDS             1..257
                     /gene="LOC144115419"
                     /coded_by="XM_077649815.1:223..996"
                     /db_xref="GeneID:144115419"
ORIGIN      
        1 mrrhhrrqtr arklfpssfs agctrsdfyt sslkpmhrst knrvssyidy cfsdvaappk
       61 knipveepsl egtkevvvae lqllasdtls gvtyntervm cllkvtfqdh cqhyvtlcev
      121 iswekissre lcaeaqlrfe stvdelevrl kkaaqvfseq qgqdtslvqi srhlqgsldh
      181 flitetalpt avqvitphvy tyknqswiyg gtqaevialk nkniewaviv cllvyylknl
      241 symhaffads yfgaktp
//
LOCUS       XP_077505942             232 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115419 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505942
VERSION     XP_077505942.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649816.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..232
                     /product="uncharacterized protein LOC144115419 isoform X4"
                     /calculated_mol_wt=26407
     CDS             1..232
                     /gene="LOC144115419"
                     /coded_by="XM_077649816.1:304..1002"
                     /db_xref="GeneID:144115419"
ORIGIN      
        1 mrrhhrrqtr arklfpsrvs syidycfsdv aappkknipv eepslegtke vvvaelqlla
       61 sdtlsgvtyn tervmcllkv tfqdhcqhyv tlceviswek issrelcaea qlrfestvde
      121 levrlkkaaq vfseqqgqdt slvqisrhlq gsldhflite talptavqvi tphvytyknq
      181 swiyggtqae vialknknie wavivcllvy ylknlsymha ffadsyfgak tp
//
LOCUS       XP_077505943             232 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115419 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077505943
VERSION     XP_077505943.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649817.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..232
                     /product="uncharacterized protein LOC144115419 isoform X4"
                     /calculated_mol_wt=26407
     CDS             1..232
                     /gene="LOC144115419"
                     /coded_by="XM_077649817.1:223..921"
                     /db_xref="GeneID:144115419"
ORIGIN      
        1 mrrhhrrqtr arklfpsrvs syidycfsdv aappkknipv eepslegtke vvvaelqlla
       61 sdtlsgvtyn tervmcllkv tfqdhcqhyv tlceviswek issrelcaea qlrfestvde
      121 levrlkkaaq vfseqqgqdt slvqisrhlq gsldhflite talptavqvi tphvytyknq
      181 swiyggtqae vialknknie wavivcllvy ylknlsymha ffadsyfgak tp
//
LOCUS       XP_077505944             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115421 [Amblyomma americanum].
ACCESSION   XP_077505944
VERSION     XP_077505944.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649818.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144115421"
                     /calculated_mol_wt=24264
     Region          7..91
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     Region          168..202
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..217
                     /gene="LOC144115421"
                     /coded_by="XM_077649818.1:185..838"
                     /db_xref="GeneID:144115421"
ORIGIN      
        1 masfneklvq evkkhtqlwd qhsklhkeag yreaawmdia aalavtveec qtrwrtlrdt
       61 ylkrkkrrra datgqwsvld relgflddfl rprtrrstln gaapaassae vevkreqpde
      121 daevresaav vaaaaapaqp walamrptrv mphrlaagga aaqalfsdpe elfclslaar
      181 lkrllprerh marmkmlqtl hdlqsgenig efalgdp
//
LOCUS       XP_077505945             427 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115422 [Amblyomma americanum].
ACCESSION   XP_077505945
VERSION     XP_077505945.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649819.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..427
                     /product="uncharacterized protein LOC144115422"
                     /calculated_mol_wt=46068
     Region          378..412
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..427
                     /gene="LOC144115422"
                     /coded_by="XM_077649819.1:166..1449"
                     /db_xref="GeneID:144115422"
ORIGIN      
        1 maaqgsreqs qysrrssrsq rrgrlprala aedrlseara srelpppppp pppqqhpwad
       61 srlsagrsta sslisrlgkh ssrppssmld eeaamrrsat svagaasgmt pvrltryygp
      121 prlgrerree eaaefgphvd gsrtwevgge mpgrrldrap laadaggsrv srrsasrqar
      181 tvaecvapas tpprparskd agdrphrtle mtfamgafvc vatlvvafvv rawmpyears
      241 dpfcrsdacl ahvrlieari drgvdpcvdf dayacsswkp asefyghasa ltnvlldnwr
      301 rwalngaara vspaevaver eqpdeddemr eaaaaeaaav avapaapaqp cpvamrpaav
      361 lphhlaagga aaqecssdpe elfclslaad lkrllprerh marmkmlqtl hdlefgenig
      421 eaalgep
//
LOCUS       XP_077505946             184 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115423 [Amblyomma americanum].
ACCESSION   XP_077505946
VERSION     XP_077505946.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649820.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..184
                     /product="uncharacterized protein LOC144115423"
                     /calculated_mol_wt=20323
     Region          <14..58
                     /region_name="GT1"
                     /note="myb-like, SANT family; cl23759"
                     /db_xref="CDD:474047"
     Region          135..169
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..184
                     /gene="LOC144115423"
                     /coded_by="XM_077649820.1:55..609"
                     /db_xref="GeneID:144115423"
ORIGIN      
        1 mvvltanrvf pgaveecqtr wrtlrdtylk rkkrrradat gqwsvldrel gflddflrpr
       61 trrstlngaa paassaevev kreqpdedae vresaavvaa aaapaqpwal amrptrvmph
      121 rlaaggaaaq alfsdpeell clslaarlkr llprerhmar mkmlqtlhdl esgenigefa
      181 lgdp
//
LOCUS       XP_077505947            1014 aa            linear   INV 25-NOV-2025
DEFINITION  lysosomal alpha-mannosidase-like [Amblyomma americanum].
ACCESSION   XP_077505947
VERSION     XP_077505947.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649821.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1014
                     /product="lysosomal alpha-mannosidase-like"
                     /calculated_mol_wt=113382
     Region          44..895
                     /region_name="PLN02701"
                     /note="alpha-mannosidase"
                     /db_xref="CDD:178304"
     Region          44..320
                     /region_name="GH38N_AMII_LAM_like"
                     /note="N-terminal catalytic domain of lysosomal
                     alpha-mannosidase and similar proteins; glycoside
                     hydrolase family 38 (GH38); cd10810"
                     /db_xref="CDD:212121"
     Site            order(53,55,58,177,179,201,299)
                     /site_type="active"
                     /db_xref="CDD:212121"
     Site            order(55..61,136..152,154..155,177,179..184,188,201..206,
                     222..224)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:212121"
     CDS             1..1014
                     /gene="LOC144115424"
                     /coded_by="XM_077649821.1:152..3196"
                     /db_xref="GeneID:144115424"
ORIGIN      
        1 mgptraivla vvclgaavss apspkrteqk cgydscpatk pdmlnvhlla hthmdlgwlk
       61 tvdqyfygtk nenanvgvry ifesvlsele sdarrrfifv etgffnlwwk klnetkkerf
      121 nalvqsglme fisggwvmnd eacvhysnti dqmtygirkl ndtfgkcgvp kigwqidpfg
      181 hsreyaslla qmgmdgyffg rvdyqdfrar kkdqrlefiw sasenlgkaa siftgilpnt
      241 ysppkgfcfd iycsddaivd dpeseeynvk ykvdefvtya kgeanfyktn nipvtmgndf
      301 nyqsaghwfs nldklihhan lvsdktkvhl lystpscylk alhqsqtswp tktddffpya
      361 sdphsywtgy ftsrpafkfl drytnnqlqv akqlgvlagp kdvteaslnv lrealavgqh
      421 hdaitgtakq avandyvkhi sigqsaadky tnyafekllp khakaqvpll vrchtlnvsa
      481 cevteastqt pvlvynpqar pvetyiripv sgenwrvlda sgsevpaqit kiptgvivip
      541 ervskadqev vflaslpalg fttyyveaan kkdisvansq tcddagvkqm vlqgkvtsat
      601 idcttgllsa iswkettvsv nqsfywyegh agnnsqfrfr asgayifrpe aqeplpvaek
      661 aelvlivkng sivqevhqkf sdwltqvirv yededfvefd wvvgsipiqd kigkeiitrf
      721 dsdlqtngvf ytdsngreil qrsrnfrptw qvdikepvsg nyypvnsrif lrdptravql
      781 tvltdrsqgg sslqdgsvel mvhrrllydd afgvgealne syfgghglvv rgthrvvvsp
      841 vaqaallhrp laqtayaapa lffapvdaat yaeqcsttcs alaaplppnv hlltlehwgd
      901 dkvllrlehf yenkdqagql skpanfslqa vfvrniesit etslavtktk eetsrlyfqa
      961 eqnnsvdfns tggdkeapfv fgpeyyvylt pmqirtflvt faadpvahmt clnr
//
LOCUS       XP_077505948             344 aa            linear   INV 25-NOV-2025
DEFINITION  stearoyl-CoA desaturase 5-like [Amblyomma americanum].
ACCESSION   XP_077505948
VERSION     XP_077505948.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649822.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..344
                     /product="stearoyl-CoA desaturase 5-like"
                     /calculated_mol_wt=39964
     Region          70..310
                     /region_name="Delta9-FADS-like"
                     /note="The Delta9 Fatty Acid Desaturase (Delta9-FADS)-like
                     CD includes the delta-9 and delta-11 acyl CoA desaturases
                     found in various eukaryotes including vertebrates,
                     insects, higher plants, and fungi. The delta-9 acyl-lipid
                     desaturases are found in a wide...; cd03505"
                     /db_xref="CDD:239582"
     Site            order(93,98,130,133..134,271,274..275)
                     /site_type="other"
                     /note="Di-iron ligands [ion binding]"
                     /db_xref="CDD:239582"
     CDS             1..344
                     /gene="LOC144115425"
                     /coded_by="XM_077649822.1:381..1415"
                     /db_xref="GeneID:144115425"
ORIGIN      
        1 maprtetati vttearskmd lepsiteddg avqlqdttep teiiwinvfk ictihvmaly
       61 glylalfeak wmtlhftify ivcsglsvta gahrlwshrs ykaklplrii lmlfncmagq
      121 ndiydwvrdh rvhhkysetd adphnvnrgf ffahvgwlmc rkhsqvtkkg kaidcsdvan
      181 dpvvmfqtry hipmtilfcf ivptvvpyry wgesrwnaff vaailrwvfq lnstwlvnsa
      241 ahiwgqkpyd knispaenli vsflaigegf hnyhhtfpwd ystselgwkf nistmfidlm
      301 akigwaydlr kptremieqr itrtgdgshp sqrhahhhhh eney
//
LOCUS       XP_077505949             344 aa            linear   INV 25-NOV-2025
DEFINITION  stearoyl-CoA desaturase 5-like [Amblyomma americanum].
ACCESSION   XP_077505949
VERSION     XP_077505949.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649823.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..344
                     /product="stearoyl-CoA desaturase 5-like"
                     /calculated_mol_wt=39964
     Region          70..310
                     /region_name="Delta9-FADS-like"
                     /note="The Delta9 Fatty Acid Desaturase (Delta9-FADS)-like
                     CD includes the delta-9 and delta-11 acyl CoA desaturases
                     found in various eukaryotes including vertebrates,
                     insects, higher plants, and fungi. The delta-9 acyl-lipid
                     desaturases are found in a wide...; cd03505"
                     /db_xref="CDD:239582"
     Site            order(93,98,130,133..134,271,274..275)
                     /site_type="other"
                     /note="Di-iron ligands [ion binding]"
                     /db_xref="CDD:239582"
     CDS             1..344
                     /gene="LOC144115425"
                     /coded_by="XM_077649823.1:239..1273"
                     /db_xref="GeneID:144115425"
ORIGIN      
        1 maprtetati vttearskmd lepsiteddg avqlqdttep teiiwinvfk ictihvmaly
       61 glylalfeak wmtlhftify ivcsglsvta gahrlwshrs ykaklplrii lmlfncmagq
      121 ndiydwvrdh rvhhkysetd adphnvnrgf ffahvgwlmc rkhsqvtkkg kaidcsdvan
      181 dpvvmfqtry hipmtilfcf ivptvvpyry wgesrwnaff vaailrwvfq lnstwlvnsa
      241 ahiwgqkpyd knispaenli vsflaigegf hnyhhtfpwd ystselgwkf nistmfidlm
      301 akigwaydlr kptremieqr itrtgdgshp sqrhahhhhh eney
//
LOCUS       XP_077505950             354 aa            linear   INV 25-NOV-2025
DEFINITION  acyl-CoA desaturase-like [Amblyomma americanum].
ACCESSION   XP_077505950
VERSION     XP_077505950.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649824.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..354
                     /product="acyl-CoA desaturase-like"
                     /calculated_mol_wt=40812
     Region          65..305
                     /region_name="Delta9-FADS-like"
                     /note="The Delta9 Fatty Acid Desaturase (Delta9-FADS)-like
                     CD includes the delta-9 and delta-11 acyl CoA desaturases
                     found in various eukaryotes including vertebrates,
                     insects, higher plants, and fungi. The delta-9 acyl-lipid
                     desaturases are found in a wide...; cd03505"
                     /db_xref="CDD:239582"
     Site            order(88,93,125,128..129,266,269..270)
                     /site_type="other"
                     /note="Di-iron ligands [ion binding]"
                     /db_xref="CDD:239582"
     CDS             1..354
                     /gene="LOC144115426"
                     /coded_by="XM_077649824.1:162..1226"
                     /db_xref="GeneID:144115426"
ORIGIN      
        1 mapqtetlve rhttapaett alvaetnhae apkkykiemv wwnvfkisli hvigiygiye
       61 myyskwqthv fgniilifsg isvtagahrl wchraykakm plriflmifn cvaaqndlye
      121 wvrdhrvhhk ysetdadphn vnrgfffahv gwlmcrkhpd vtekgkhidc sdimadpvar
      181 fqkqyymrlv mlfsfylptv lpawlwgesy wnafctvtml rlilslnftw svnsfahlwg
      241 tkpydksisp tetpwvsffa vgegfhnfhh tfpwdystse lgwrlnmstm fidimaklgq
      301 aydlktvske avlarklrtg dgtrlprrfr kmaavatenn attttpavts tsqg
//
LOCUS       XP_077505951             343 aa            linear   INV 25-NOV-2025
DEFINITION  acyl-CoA Delta-9 desaturase-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505951
VERSION     XP_077505951.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649825.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..343
                     /product="acyl-CoA Delta-9 desaturase-like isoform X1"
                     /calculated_mol_wt=39316
     Region          36..324
                     /region_name="OLE1"
                     /note="Fatty-acid desaturase [Lipid transport and
                     metabolism]; COG1398"
                     /db_xref="CDD:441008"
     CDS             1..343
                     /gene="LOC144115428"
                     /coded_by="XM_077649825.1:128..1159"
                     /db_xref="GeneID:144115428"
ORIGIN      
        1 mttsavttas vterkkhdgd vqerqpqrdn ssdpeseavs qepykteivw rnvflflflh
       61 igaacglyca lftakwqtnl mafiglgisg vgssvaahrl wchrtfkakl plrivlmman
      121 cfalqndiye wtrdhrvhhk ysdtdadphn inrgfffahm gwlmckkhpe vfrkgatvdc
      181 sdvladpvva fqrkfyvplv tffcfilptw vpmwlvgetf wnahlvnaml rytvslhmtw
      241 lvnsaahtwg yrpydkdihp tenpaisfgl fgegfhnfhh tfpqdyksse fgpllnpati
      301 fidlmakigq aydlkttpkh liekriertg dkkqhimraf vst
//
LOCUS       XP_077505952             344 aa            linear   INV 25-NOV-2025
DEFINITION  acyl-CoA Delta-9 desaturase-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505952
VERSION     XP_077505952.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649826.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..344
                     /product="acyl-CoA Delta-9 desaturase-like isoform X2"
                     /calculated_mol_wt=39485
     Region          37..325
                     /region_name="OLE1"
                     /note="Fatty-acid desaturase [Lipid transport and
                     metabolism]; COG1398"
                     /db_xref="CDD:441008"
     CDS             1..344
                     /gene="LOC144115428"
                     /coded_by="XM_077649826.1:167..1201"
                     /db_xref="GeneID:144115428"
ORIGIN      
        1 mirtsavtta svterkkhdg dvqerqpqrd nssdpeseav sqepykteiv wrnvflflfl
       61 higaacglyc alftakwqtn lmafiglgis gvgssvaahr lwchrtfkak lplrivlmma
      121 ncfalqndiy ewtrdhrvhh kysdtdadph ninrgfffah mgwlmckkhp evfrkgatvd
      181 csdvladpvv afqrkfyvpl vtffcfilpt wvpmwlvget fwnahlvnam lrytvslhmt
      241 wlvnsaahtw gyrpydkdih ptenpaisfg lfgegfhnfh htfpqdykss efgpllnpat
      301 ifidlmakig qaydlkttpk hliekriert gdkkqhimra fvst
//
LOCUS       XP_077505953             333 aa            linear   INV 25-NOV-2025
DEFINITION  acyl-CoA Delta-9 desaturase-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505953
VERSION     XP_077505953.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649827.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..333
                     /product="acyl-CoA Delta-9 desaturase-like isoform X1"
                     /calculated_mol_wt=38497
     Region          25..311
                     /region_name="OLE1"
                     /note="Fatty-acid desaturase [Lipid transport and
                     metabolism]; COG1398"
                     /db_xref="CDD:441008"
     CDS             1..333
                     /gene="LOC144115429"
                     /coded_by="XM_077649827.1:226..1227"
                     /db_xref="GeneID:144115429"
ORIGIN      
        1 msiitshstt vqqqqqtvsq epekatqpaf ktevvwrnaa vfailhsmal yglylavtrr
       61 amwqtavfat vwgvcaglgv tagahrlwsh raykarfplr vllmifncma cqndlyewtr
      121 dhrvhhkfse sdadphnvnr gfffahvgwl mckkhpdvmr kgkavdcsdl lkdpvirfqk
      181 nyyvplttff cfylptvlph hlfgesywva ffvatmlryv tslnftwlvn saahlwgnrp
      241 ydknispaen rfvawaaige gfhnyhhtfp wdystselgw tinfttmfid amaklglayd
      301 lktvpkdlie krksrtgdhs ydymnghkhn hhh
//
LOCUS       XP_077505954             389 aa            linear   INV 25-NOV-2025
DEFINITION  acyl-CoA desaturase-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505954
VERSION     XP_077505954.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649828.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..389
                     /product="acyl-CoA desaturase-like isoform X2"
                     /calculated_mol_wt=44806
     Region          81..367
                     /region_name="OLE1"
                     /note="Fatty-acid desaturase [Lipid transport and
                     metabolism]; COG1398"
                     /db_xref="CDD:441008"
     CDS             1..389
                     /gene="LOC144115429"
                     /coded_by="XM_077649828.1:303..1472"
                     /db_xref="GeneID:144115429"
ORIGIN      
        1 msndmktqkt vtpttpadll prngtglrkr dldiidamdl ikqteyvsls ktdlldheii
       61 tshsttvqqq qqtvsqepek atqpafktev vwrnaavfai lhsmalygly lavtrramwq
      121 tavfatvwgv caglgvtaga hrlwshrayk arfplrvllm ifncmacqnd lyewtrdhrv
      181 hhkfsesdad phnvnrgfff ahvgwlmckk hpdvmrkgka vdcsdllkdp virfqknyyv
      241 plttffcfyl ptvlphhlfg esywvaffva tmlryvtsln ftwlvnsaah lwgnrpydkn
      301 ispaenrfva waaigegfhn yhhtfpwdys tselgwtinf ttmfidamak lglaydlktv
      361 pkdliekrks rtgdhsydym nghkhnhhh
//
LOCUS       XP_077505955             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115430 [Amblyomma americanum].
ACCESSION   XP_077505955
VERSION     XP_077505955.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649829.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144115430"
                     /calculated_mol_wt=19991
     CDS             1..217
                     /gene="LOC144115430"
                     /coded_by="XM_077649829.1:145..798"
                     /db_xref="GeneID:144115430"
ORIGIN      
        1 mggqvlawll iaacvgqafs gylvnrrgyg gggyggggyg gggyggsgyg gggystrssy
       61 gggggggygg gyggsggygv tsayrgrggg gyggggygsg ggyrsyggsy ggyrgggygg
      121 gktytssvsy gggryggggy sggsygggay ggggyggggy ggggyggggy ggssygksgy
      181 vavrshggrg ydrgygsgyg ggygggsggy gksyykw
//
LOCUS       XP_077505956             237 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115431 [Amblyomma americanum].
ACCESSION   XP_077505956
VERSION     XP_077505956.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649830.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..237
                     /product="uncharacterized protein LOC144115431"
                     /calculated_mol_wt=23596
     CDS             1..237
                     /gene="LOC144115431"
                     /coded_by="XM_077649830.1:256..969"
                     /db_xref="GeneID:144115431"
ORIGIN      
        1 mlknvvqala lgalfvflaa ghplpeepes spaaearadd iahaveqvsa dgvaspdgsp
       61 eaavvetaaa gavpgeaage vapssdgase vaaaaggahg plvvpldaga apvphvsgav
      121 sgpgyehesh qqaarqdafs eaaqgaqqgs haaesgeafk ktegfenqgg frkqegfsdk
      181 ssnrygsgff qgsegqhefd rgdqhsfgva gygvhrssag vaggpnyqel lkvgfvp
//
LOCUS       XP_077505957             176 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115432 [Amblyomma americanum].
ACCESSION   XP_077505957
VERSION     XP_077505957.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649831.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..176
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..176
                     /product="uncharacterized protein LOC144115432"
                     /calculated_mol_wt=16625
     CDS             1..176
                     /gene="LOC144115432"
                     /coded_by="XM_077649831.1:71..601"
                     /db_xref="GeneID:144115432"
ORIGIN      
        1 mpsgvitlpp svcrlglean ppstkmltlv lvllgvsacf agdvllggag lgagglgagv
       61 gygagagypv gagygggfga gynqqaggaq ggfssgaagh qqgsgafdtg nayknvqgys
      121 nqgghrsstg fsqsssnryg tgfgqgsggy nqgasgaqgs ygkqgfgtqy ggvgay
//
LOCUS       XP_077505958             204 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115433 [Amblyomma americanum].
ACCESSION   XP_077505958
VERSION     XP_077505958.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649832.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..204
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..204
                     /product="uncharacterized protein LOC144115433"
                     /calculated_mol_wt=19053
     CDS             1..204
                     /gene="LOC144115433"
                     /coded_by="XM_077649832.1:105..719"
                     /db_xref="GeneID:144115433"
ORIGIN      
        1 mkffasialf avvacaafae eavkkdekke ekkdveargg vlggvggyga gvapglvaag
       61 lggpglvggg avgnpalvga glghgvglgg vglghgvggg fqsgygasag ghqagfqkga
      121 sghnqgsgaf aggssqrnvn aynnnqgysh ssgfsasdtk kfgaghqqgs sgfqggaagh
      181 qagfgqssfg kaagvghagv glhg
//
LOCUS       XP_077505959             189 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115434 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077505959
VERSION     XP_077505959.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649833.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..189
                     /product="uncharacterized protein LOC144115434 isoform X1"
                     /calculated_mol_wt=17829
     CDS             1..189
                     /gene="LOC144115434"
                     /coded_by="XM_077649833.1:152..721"
                     /db_xref="GeneID:144115434"
ORIGIN      
        1 mktqtfatit lfafvaciaf aeeaakkedk kdvegrggvl ggvgygagvg pglvgpgvvg
       61 npalvgaglg hgvglggvgl ghgggfqsgy gasagghqag fqkgaaghnq gsgafaggas
      121 hrnvnaynnn qgyshssgfs ssdsksfgtg hqqgssgfqg gaaghqagfg qssfgkaagv
      181 ghagvglhg
//
LOCUS       XP_077505960             187 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115434 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077505960
VERSION     XP_077505960.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649834.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="uncharacterized protein LOC144115434 isoform X2"
                     /calculated_mol_wt=17600
     CDS             1..187
                     /gene="LOC144115434"
                     /coded_by="XM_077649834.1:153..716"
                     /db_xref="GeneID:144115434"
ORIGIN      
        1 mktfatitlf afvaciafae eaakkedkkd vegrggvlgg vgygagvgpg lvgpgvvgnp
       61 alvgaglghg vglggvglgh gggfqsgyga sagghqagfq kgaaghnqgs gafaggashr
      121 nvnaynnnqg yshssgfsss dsksfgtghq qgssgfqgga aghqagfgqs sfgkaagvgh
      181 agvglhg
//
LOCUS       XP_077505961             205 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115435 [Amblyomma americanum].
ACCESSION   XP_077505961
VERSION     XP_077505961.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649835.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..205
                     /product="uncharacterized protein LOC144115435"
                     /calculated_mol_wt=18826
     CDS             1..205
                     /gene="LOC144115435"
                     /coded_by="XM_077649835.1:110..727"
                     /db_xref="GeneID:144115435"
ORIGIN      
        1 mkfaaiavla lvacvavaed tkkavakeek kdvearggvl ggvglggvgy gagvapgvvg
       61 tglggpalah pglvgagvvg npalvgaglg hgvglghgvg ggyqtgygaa agghqsgfqg
      121 aaaghnqgsg afaggaahss vnayknnqgy shssgfsasd sktfgagqkq gssafntgaa
      181 ghqsgfgkta hgatggvgav glgih
//
LOCUS       XP_077505962             231 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115437 [Amblyomma americanum].
ACCESSION   XP_077505962
VERSION     XP_077505962.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649836.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..231
                     /product="uncharacterized protein LOC144115437"
                     /calculated_mol_wt=20894
     CDS             1..231
                     /gene="LOC144115437"
                     /coded_by="XM_077649836.1:149..844"
                     /db_xref="GeneID:144115437"
ORIGIN      
        1 mkplclavvv aiaclalaek kggkdekveg rgglvggygg gygpgvggig gvpvggagig
       61 gvpvggvgig gvpvggvgig gvpvggvgir pglggvgygg glgqgglghg glghgglghg
      121 glghgglghg glvgggagfg qsaggfqggf kqgatgfnqg sggfasggsh rnvqgynnqq
      181 gyssstgfsk tdsnrygtgf gqhsggfagg asgqkagfgs qsfgqvggvg y
//
LOCUS       XP_077505963             171 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115438 [Amblyomma americanum].
ACCESSION   XP_077505963
VERSION     XP_077505963.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649837.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..171
                     /product="uncharacterized protein LOC144115438"
                     /calculated_mol_wt=15919
     CDS             1..171
                     /gene="LOC144115438"
                     /coded_by="XM_077649837.1:133..648"
                     /db_xref="GeneID:144115438"
ORIGIN      
        1 mkalclavva aiaclalaek kagkeekveg rgglgggygg gygpgvggig gvpvggvgyg
       61 gglghggfgh gghgggagyg qsaggfqggf rqgaagfnqg sggfssgaad rnvqgynnqq
      121 gyssssgfsk tdsdrygtgf gqqsggfagg ssghkaghgs qgfgqvggvg y
//
LOCUS       XP_077505964             174 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115439 [Amblyomma americanum].
ACCESSION   XP_077505964
VERSION     XP_077505964.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649838.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..174
                     /product="uncharacterized protein LOC144115439"
                     /calculated_mol_wt=16483
     CDS             1..174
                     /gene="LOC144115439"
                     /coded_by="XM_077649838.1:150..674"
                     /db_xref="GeneID:144115439"
ORIGIN      
        1 mkflsfavva avaclavagp aakkeaaaad kkeekiegrg gliggpvgyg ggygggyggg
       61 yggdfggpgf ggagllggag ygqtaggyqs gfaggakgfn qgsgafaggd sfrnvqgynn
      121 qqgyssstgf sktdsnrygt gfgqhssgfg aggaghqagf gqagfgqvgg ighy
//
LOCUS       XP_077505966             131 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115440 [Amblyomma americanum].
ACCESSION   XP_077505966
VERSION     XP_077505966.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649840.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..131
                     /product="uncharacterized protein LOC144115440"
                     /calculated_mol_wt=13636
     CDS             1..131
                     /gene="LOC144115440"
                     /coded_by="XM_077649840.1:142..537"
                     /db_xref="GeneID:144115440"
ORIGIN      
        1 marklyilaa lvavaalaya eekpaaeekk ddvegrigfg ggfgqasggn rygfnrgqsg
       61 fnqgsggfds akrfnnaqgf rnrdgyrtnq gydqtsfnry gsggagfntg fnrgaggafn
      121 qygqqgggyl g
//
LOCUS       XP_077505967             345 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115441 [Amblyomma americanum].
ACCESSION   XP_077505967
VERSION     XP_077505967.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649841.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..345
                     /product="uncharacterized protein LOC144115441"
                     /calculated_mol_wt=30888
     CDS             1..345
                     /gene="LOC144115441"
                     /coded_by="XM_077649841.1:107..1144"
                     /db_xref="GeneID:144115441"
ORIGIN      
        1 mrtllilssi lalclvvlae dkpaddkaae attvptaaaa ggddkkegde kiegrglfls
       61 ggglggcppc pcaygpgvgg giggagygsg fggsygagga ygggygggyr ggygggyggg
      121 ygggvrpgvg lggasyvagg gvgpglgggv gpglgggvgp gigggvgpgi ggvgpgiggv
      181 gpgvgkdvvg gpvgpigggg vgvgpgigkg vvggpvvpvg gavgvgpgvg gvvpgvgvgp
      241 gygggygggf ggaaassgye saggfankaa gqqagqgsfd ynvggsdkkv yghstsygds
      301 ksfgqseseg fnraqgqgsh gssfnkeaag sgaethssgk evvvg
//
LOCUS       XP_077505968             332 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial amidoxime-reducing component 1-like [Amblyomma
            americanum].
ACCESSION   XP_077505968
VERSION     XP_077505968.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649842.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..332
                     /product="mitochondrial amidoxime-reducing component
                     1-like"
                     /calculated_mol_wt=37003
     Region          44..322
                     /region_name="YcbX"
                     /note="N-hydroxylaminopurine reductase subunit YcbX,
                     contains MOSC domain [Nucleotide transport and metabolism,
                     Defense mechanisms]; COG3217"
                     /db_xref="CDD:442450"
     CDS             1..332
                     /gene="LOC144115442"
                     /coded_by="XM_077649842.1:492..1490"
                     /db_xref="GeneID:144115442"
ORIGIN      
        1 mvssssgmdp qssialitvl ggvvgaavff wlkrmaagnk fvrvgrvani vihpikslag
       61 vevpyadctv agavykglkd rhmlivrddy fvsmreeprl gmvrvafded mltltltvdg
      121 yppltvkacs aeeqgkpsft irvrkvsyka vevsqeasew frnylkhgdv rlvmvlldqe
      181 tiargkngia pvacqdessf qvlskeslek llsrlppgsg iqrrnfrptl fiegcephae
      241 dhwkrfrisd aemaflnrtt rcilttvnqd tgdrtdkepl ltlrkyridr seqgrqkyqs
      301 lplfgigsih vrdgriavgd evyalqspep ll
//
LOCUS       XP_077505969             317 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115443 [Amblyomma americanum].
ACCESSION   XP_077505969
VERSION     XP_077505969.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649843.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..317
                     /product="uncharacterized protein LOC144115443"
                     /calculated_mol_wt=28335
     CDS             1..317
                     /gene="LOC144115443"
                     /coded_by="XM_077649843.1:25..978"
                     /db_xref="GeneID:144115443"
ORIGIN      
        1 mwfqcgraql lpweatream hiksagacsp vtsrprpvsa mkplcavall alcalasagk
       61 kekveargal lgggvggccp ctggvgvglg gaglgaglgg gssyssgsys sgsgygsgag
      121 aglagaglas gaglasgagl asgaglasga glasgaglas gaglasgagl asgaglasgv
      181 gvgagvplas pgvgvsgagl gvggaglgvg gvgygtglgg gfgsasgvsg fkqgsgfnrq
      241 agankagqgs faynvggsds nkyghsssyg dsksfgssas egfnrasgqg sqgssfnkaa
      301 agsgaqssss gskvvsg
//
LOCUS       XP_077505970             239 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115444 [Amblyomma americanum].
ACCESSION   XP_077505970
VERSION     XP_077505970.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649844.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..239
                     /product="uncharacterized protein LOC144115444"
                     /calculated_mol_wt=21545
     Region          <166..>235
                     /region_name="ser_rich_anae_1"
                     /note="serine-rich protein; NF033849"
                     /db_xref="CDD:468206"
     CDS             1..239
                     /gene="LOC144115444"
                     /coded_by="XM_077649844.1:91..810"
                     /db_xref="GeneID:144115444"
ORIGIN      
        1 mkaiccliaa aclvgsafca etkkkndkkd dgkiegrglf hhgaggvgfp ggcppcpcay
       61 ggaggigggy ggaggvgggf sgaggvggsy aagqggagyv agghgigggy gaggvgpiga
      121 ggpglvgggp gvigggpiva gpgivggaag fsgfkggagf ankaagqqag qgsfaynvgg
      181 sdkmeyghss sygdsksfgm sssegfnmah gqgssgsafn kqaagagaia hsagakvvg
//
LOCUS       XP_077505971             157 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115445 [Amblyomma americanum].
ACCESSION   XP_077505971
VERSION     XP_077505971.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649845.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..157
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..157
                     /product="uncharacterized protein LOC144115445"
                     /calculated_mol_wt=15822
     CDS             1..157
                     /gene="LOC144115445"
                     /coded_by="XM_077649845.1:147..620"
                     /db_xref="GeneID:144115445"
ORIGIN      
        1 mcrllpfaaf lallglalaa paeemapape aaaeplpaad akpasaddka vegrtgyfpv
       61 ygggaygagf nrggsfglgs ygnnygqggf gynyggtnrr dygnvqtygd reafgvsqsa
      121 ganrrygqgs fgnaynnqah gagansygag yqggylg
//
LOCUS       XP_077505972             189 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115446 [Amblyomma americanum].
ACCESSION   XP_077505972
VERSION     XP_077505972.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649846.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..189
                     /product="uncharacterized protein LOC144115446"
                     /calculated_mol_wt=18473
     CDS             1..189
                     /gene="LOC144115446"
                     /coded_by="XM_077649846.1:137..706"
                     /db_xref="GeneID:144115446"
ORIGIN      
        1 mrgiflialg mavlaaalaa pakdkkddpa aggqgedveg rigfgrgrgr grghhhrggg
       61 gggfgggpgf ggpglkgpgg lgggygpgfg ggsgpglgga qygsgfnqgg sfqtgsegnr
      121 ygqggfnynv ggsnrrdygn vqtygdresf glsqneganr atgqssfgne yknqeqgsgg
      181 kqfstgfag
//
LOCUS       XP_077505973             189 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115446 [Amblyomma americanum].
ACCESSION   XP_077505973
VERSION     XP_077505973.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649847.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..189
                     /product="uncharacterized protein LOC144115446"
                     /calculated_mol_wt=18473
     CDS             1..189
                     /gene="LOC144115446"
                     /coded_by="XM_077649847.1:137..706"
                     /db_xref="GeneID:144115446"
ORIGIN      
        1 mrgiflialg mavlaaalaa pakdkkddpa aggqgedveg rigfgrgrgr grghhhrggg
       61 gggfgggpgf ggpglkgpgg lgggygpgfg ggsgpglgga qygsgfnqgg sfqtgsegnr
      121 ygqggfnynv ggsnrrdygn vqtygdresf glsqneganr atgqssfgne yknqeqgsgg
      181 kqfstgfag
//
LOCUS       XP_077505974             131 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115448 [Amblyomma americanum].
ACCESSION   XP_077505974
VERSION     XP_077505974.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649848.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..131
                     /product="uncharacterized protein LOC144115448"
                     /calculated_mol_wt=12900
     CDS             1..131
                     /gene="LOC144115448"
                     /coded_by="XM_077649848.1:147..542"
                     /db_xref="GeneID:144115448"
ORIGIN      
        1 mktifafaaa lallglavaa paeegkkeev egrigfgggg fgsgfnqggs fgvgshgtrh
       61 gqggfgynvg gsnrrdygna aaygdresfg lsgssghnrq fgqgssgnrf hnnafgaggn
      121 rfasggggyl g
//
LOCUS       XP_077505975             294 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115449 [Amblyomma americanum].
ACCESSION   XP_077505975
VERSION     XP_077505975.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649849.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 29% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..294
                     /product="uncharacterized protein LOC144115449"
                     /calculated_mol_wt=33145
     Region          63..145
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     CDS             1..294
                     /gene="LOC144115449"
                     /coded_by="XM_077649849.1:1..885"
                     /db_xref="GeneID:144115449"
ORIGIN      
        1 mpeaahhqav qvgslglcsg slrqrdpalf sgtdnrdaed wltsyerlst ynnwddttkl
       61 nnvffyltgv anlwfrnhea dlstwtafka nltevfggpa vrklraehrf rersrqsget
      121 ftsyiedvvn lcsrvnpqms eedkikhilk iiedddfqml lsrdlrtvae vvslcqsyde
      181 lrkqrlitrq haaavvesla glsaapdrsq llvhmkdfvr eevagqllll pspsvqspfl
      241 apdlptaise qlqplqcppr pfdcvgiyiy gplpytaagn rwvivaidhl trmq
//
LOCUS       XP_077505976             121 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115450 [Amblyomma americanum].
ACCESSION   XP_077505976
VERSION     XP_077505976.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649850.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..121
                     /product="uncharacterized protein LOC144115450"
                     /calculated_mol_wt=13041
     CDS             1..121
                     /gene="LOC144115450"
                     /coded_by="XM_077649850.1:130..495"
                     /db_xref="GeneID:144115450"
ORIGIN      
        1 mavlkkvlfl laagivildi ldikkcaaer sgsdlekgph avgnvqrlve elgyirdkrd
       61 lhkegrhqag rthagrtqtg rthtgrtrne gphagrakag rthagrthag rtngdggrrh
      121 h
//
LOCUS       XP_077505977             231 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115451 [Amblyomma americanum].
ACCESSION   XP_077505977
VERSION     XP_077505977.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649851.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..231
                     /product="uncharacterized protein LOC144115451"
                     /calculated_mol_wt=20792
     CDS             1..231
                     /gene="LOC144115451"
                     /coded_by="XM_077649851.1:145..840"
                     /db_xref="GeneID:144115451"
ORIGIN      
        1 mkffvtaavc alvacavyae eakkkdekkd vegrggilga gvglggvggy gagvgpglvg
       61 aglggaglgg vglagagygg agavgaglgg agligagvpg agvvanpalv gaglghgvgv
      121 ghgigggyqs gygssagghq tgyqggasgh nqgsgafagg ashskvnsys nnqgyshssg
      181 fsasdsksfg tghkqgstgf nsgaaghqag fgqssfgkaa gvgvggvglh g
//
LOCUS       XP_077505978             549 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115452 [Amblyomma americanum].
ACCESSION   XP_077505978
VERSION     XP_077505978.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649852.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..549
                     /product="uncharacterized protein LOC144115452"
                     /calculated_mol_wt=60031
     Region          <83..411
                     /region_name="PHA03377"
                     /note="EBNA-3C; Provisional"
                     /db_xref="CDD:177614"
     Region          <278..>383
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     CDS             1..549
                     /gene="LOC144115452"
                     /coded_by="XM_077649852.1:180..1829"
                     /db_xref="GeneID:144115452"
ORIGIN      
        1 milpitalrl avaaavenvt tplpsdfpdg dviiwrrsde gadnsswlpa llppstipgs
       61 gqvdpgfapd yhtrirfpqn stdvltvsdi aeiermlvkl ndtgnatstg geapaqrarr
      121 svddddddga nyipprsaqk pesfpeaisv pgysegaqlr papqeddats effgkflgrs
      181 aanledgrad aqtepqpvpt yapvlkaveg ddngeqerdr rqatptppvt stgednistt
      241 lapvepttlq spqsttiasv etssensqpa ttatslnpee yptemstvqt tsaseapqpq
      301 peypsypvpp qgfypqypqg iypqypqgpy pqypqgpypq ypqgfypryp qgpyhqyphg
      361 fypnspgiid pgfapnpnar lfqtaleeqr rrgvwqplpa fpqgtwqqyp gssdiidpgf
      421 aprpidqlfq raleqqrqqq mqqgagqags aqeenqrpss peeqvnvvyv dgdaqghpsn
      481 vpgidrgtvp lrtedalfke vleeekrqqh eqqqqqpspp eqpvenvnpp evvpteqslp
      541 tplpesnre
//
LOCUS       XP_077505979             152 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115453 [Amblyomma americanum].
ACCESSION   XP_077505979
VERSION     XP_077505979.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649853.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..152
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..152
                     /product="uncharacterized protein LOC144115453"
                     /calculated_mol_wt=13957
     CDS             1..152
                     /gene="LOC144115453"
                     /coded_by="XM_077649853.1:92..550"
                     /db_xref="GeneID:144115453"
ORIGIN      
        1 malskvvifl gavllfavct shaledvger kggrgglgsr gsggygsgsg gfgdkgglgs
       61 gglggrgshg glqghhgsgl dshghghdgr nhggsrqggl gglgsggrgd ygdssgfggs
      121 ggrgshggfg gggrggsggl ggnrggsrgg ry
//
LOCUS       XP_077505980             448 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115454 [Amblyomma americanum].
ACCESSION   XP_077505980
VERSION     XP_077505980.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649854.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..448
                     /product="uncharacterized protein LOC144115454"
                     /calculated_mol_wt=45207
     CDS             1..448
                     /gene="LOC144115454"
                     /coded_by="XM_077649854.1:137..1483"
                     /db_xref="GeneID:144115454"
ORIGIN      
        1 mtgnysfegl rrkrpmvcvc vslllalava saaatprqtp eepklellds eietttdedg
       61 efiegrivgg kpvsvlrkre hggaatskts ggsgssgaga qkprgnsass aapgsgaqrp
      121 saagrphpgd rlkldssswk pslsgssags gnsrpsfssg nqpssspptg gssfnrprpg
      181 dygqasqgsn pthgtngfen ehrggmpnqs pdnggsvpwn hqgvspgysa ypapvtsayt
      241 ddepsgyspn lvptpniltp snepppshps nptnspgglr ggivighppg nsfsqpdsgv
      301 nrpsvhgddn gprlegglii gspptnslgv qsggfnnhpp pqdidlarrg vvgpqhpqqg
      361 agsfglpsip snpfapanpg fqgpgslplp fgaglfnpgl mniqkaveha aagvlgptga
      421 gpnpgaasvf grtstfgqms sfgftdrk
//
LOCUS       XP_077505981             261 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115455 [Amblyomma americanum].
ACCESSION   XP_077505981
VERSION     XP_077505981.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649855.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..261
                     /product="uncharacterized protein LOC144115455"
                     /calculated_mol_wt=24371
     CDS             1..261
                     /gene="LOC144115455"
                     /coded_by="XM_077649855.1:138..923"
                     /db_xref="GeneID:144115455"
ORIGIN      
        1 mmkaalvlav glavmafvsc adepevaate apaagstepp ttepaadgkg kkegkekveg
       61 rggifgpglg ygpgiglgyg gygypglgfg gvgypglgyg glgygaglgy gglgygagyg
      121 ggygglgygr lgyggpgygg lgygglgygg lgygglgygg lgygglgygg agyggygggy
      181 ygagrgygqg gfnygggeaq krvyghtsty gqsssfglqa nagsaygagh qaggagfeka
      241 ahaggaaggq yggghiggvv g
//
LOCUS       XP_077505982             144 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115456 [Amblyomma americanum].
ACCESSION   XP_077505982
VERSION     XP_077505982.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649856.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..144
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..144
                     /product="uncharacterized protein LOC144115456"
                     /calculated_mol_wt=16134
     Region          10..56
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(12,15,27,29,32,35,51,54)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438403"
     CDS             1..144
                     /gene="LOC144115456"
                     /coded_by="XM_077649856.1:171..605"
                     /db_xref="GeneID:144115456"
ORIGIN      
        1 mertdydtvf ncpvclspkq gmvfglcqhf vcadclydaa elsllkpfss cpickaknvf
       61 peerpdipdt trqlmeilgv vkcgrklcge emwtwekeqh dsachgisrr atgrrdasps
      121 ssrkvdtrtt krlrsmnngs nkvn
//
LOCUS       XP_077505983             144 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115456 [Amblyomma americanum].
ACCESSION   XP_077505983
VERSION     XP_077505983.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649857.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..144
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..144
                     /product="uncharacterized protein LOC144115456"
                     /calculated_mol_wt=16134
     Region          10..56
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(12,15,27,29,32,35,51,54)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438403"
     CDS             1..144
                     /gene="LOC144115456"
                     /coded_by="XM_077649857.1:171..605"
                     /db_xref="GeneID:144115456"
ORIGIN      
        1 mertdydtvf ncpvclspkq gmvfglcqhf vcadclydaa elsllkpfss cpickaknvf
       61 peerpdipdt trqlmeilgv vkcgrklcge emwtwekeqh dsachgisrr atgrrdasps
      121 ssrkvdtrtt krlrsmnngs nkvn
//
LOCUS       XP_077505985            1012 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505985
VERSION     XP_077505985.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649859.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1012
                     /product="protein argonaute-2-like isoform X1"
                     /calculated_mol_wt=112526
     Region          <14..>90
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     Region          151..1011
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          539..968
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(671,675,687..690,693,702,705,709,713)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(740,742,818,958)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..1012
                     /gene="LOC144115462"
                     /coded_by="XM_077649859.1:494..3532"
                     /db_xref="GeneID:144115462"
ORIGIN      
        1 mgkgerkdkk kndsaggppa vpsasggnqa tapapspagt prdlfaqvlq qgaapspqpr
       61 avsrgpapaa vpraqptaqp sasrpsygap aaagdanvaa ssprpasaaa avsrsqpaaa
      121 alppsppcts pvavteqtpe agdqatpggd vsikelertl pshfprrpah gqlgrtiqll
      181 anhfsieipt gsvyhydvdi ssetaketkv peqknyrcls tkinriviel lvkkyrqdla
      241 ncipafdgrk nlytrrqlnf rertftvdie edqrsqkfii kiqyaatvnl dalhgvfqkr
      301 vqtvpqevlq aidivlrhsp sinlapvgrs ffrppgpneh ndlgggrevw fgyyasvrpa
      361 qwkpmlnvdm satafyeslp lvdfmcrffs dsrrvltpad lrslrdnqya rlnrelkglr
      421 vkvthlpypr kykvvkitre aakdiyfese gsqisvadyf qsryrrlsyp nfpcvqsgsp
      481 thpvyiplev celaegqhcr kkleesqtae mmkrtakppa krfleirqsv rdmvstsdky
      541 lrefgvkinp eptqvvgril dppslvfenn smckprdgtw dlrgqrfyka msmtqwivln
      601 vsrfaqkdcl dnfikmliri gqelgmriaq plavitfdtn rkpmrtvlte qrkqrpqlem
      661 vvavitkatn yaeikqvaet elslrtqcil dnnvvqkcna aliqnlcqki nakmgginns
      721 lliqekpkll hrpvivigad vshpspgdki rpsiaacvgs ldsvpskfha tirvqiedsk
      781 akarveiikd lkdmikelll vfyrttghkp lrivfyrdgv segqflevrn hevsairlac
      841 aemcptetye ppltfivvqk rhhtrfmpan drdgvgkfrn vppgttvdsv vthpldfdff
      901 lcshfgiqgt srpahyyivw ddsnfsaddl qklsfylcht ysrcarsvsi papvyyahla
      961 afrakhhias kletsgavsq ssegggdavl ttaqyveavk vlqelqasmy fv
//
LOCUS       XP_077505986            1012 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505986
VERSION     XP_077505986.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649860.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1012
                     /product="protein argonaute-2-like isoform X1"
                     /calculated_mol_wt=112526
     Region          <14..>90
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     Region          151..1011
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          539..968
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(671,675,687..690,693,702,705,709,713)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(740,742,818,958)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..1012
                     /gene="LOC144115462"
                     /coded_by="XM_077649860.1:494..3532"
                     /db_xref="GeneID:144115462"
ORIGIN      
        1 mgkgerkdkk kndsaggppa vpsasggnqa tapapspagt prdlfaqvlq qgaapspqpr
       61 avsrgpapaa vpraqptaqp sasrpsygap aaagdanvaa ssprpasaaa avsrsqpaaa
      121 alppsppcts pvavteqtpe agdqatpggd vsikelertl pshfprrpah gqlgrtiqll
      181 anhfsieipt gsvyhydvdi ssetaketkv peqknyrcls tkinriviel lvkkyrqdla
      241 ncipafdgrk nlytrrqlnf rertftvdie edqrsqkfii kiqyaatvnl dalhgvfqkr
      301 vqtvpqevlq aidivlrhsp sinlapvgrs ffrppgpneh ndlgggrevw fgyyasvrpa
      361 qwkpmlnvdm satafyeslp lvdfmcrffs dsrrvltpad lrslrdnqya rlnrelkglr
      421 vkvthlpypr kykvvkitre aakdiyfese gsqisvadyf qsryrrlsyp nfpcvqsgsp
      481 thpvyiplev celaegqhcr kkleesqtae mmkrtakppa krfleirqsv rdmvstsdky
      541 lrefgvkinp eptqvvgril dppslvfenn smckprdgtw dlrgqrfyka msmtqwivln
      601 vsrfaqkdcl dnfikmliri gqelgmriaq plavitfdtn rkpmrtvlte qrkqrpqlem
      661 vvavitkatn yaeikqvaet elslrtqcil dnnvvqkcna aliqnlcqki nakmgginns
      721 lliqekpkll hrpvivigad vshpspgdki rpsiaacvgs ldsvpskfha tirvqiedsk
      781 akarveiikd lkdmikelll vfyrttghkp lrivfyrdgv segqflevrn hevsairlac
      841 aemcptetye ppltfivvqk rhhtrfmpan drdgvgkfrn vppgttvdsv vthpldfdff
      901 lcshfgiqgt srpahyyivw ddsnfsaddl qklsfylcht ysrcarsvsi papvyyahla
      961 afrakhhias kletsgavsq ssegggdavl ttaqyveavk vlqelqasmy fv
//
LOCUS       XP_077505987             933 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505987
VERSION     XP_077505987.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649861.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..933
                     /product="protein argonaute-2-like isoform X2"
                     /calculated_mol_wt=106016
     Region          58..932
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          460..889
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(592,596,608..611,614,623,626,630,634)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(661,663,739,879)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..933
                     /gene="LOC144115462"
                     /coded_by="XM_077649861.1:17..2818"
                     /db_xref="GeneID:144115462"
ORIGIN      
        1 mvhlnggggp qtadkttefl wqcalpqlra itkkmhspvh tygevslpeq vqdvlrrgpk
       61 faveprqsas ellamelert lpshfprrpa hgqlgrtiql lanhfsieip tgsvyhydvd
      121 issetaketk vpeqknyrcl stkinrivie llvkkyrqdl ancipafdgr knlytrrqln
      181 frertftvdi eedqrsqkfi ikiqyaatvn ldalhgvfqk rvqtvpqevl qaidivlrhs
      241 psinlapvgr sffrppgpne hndlgggrev wfgyyasvrp aqwkpmlnvd msatafyesl
      301 plvdfmcrff sdsrrvltpa dlrslrdnqy arlnrelkgl rvkvthlpyp rkykvvkitr
      361 eaakdiyfes egsqisvady fqsryrrlsy pnfpcvqsgs pthpvyiple vcelaegqhc
      421 rkkleesqta emmkrtakpp akrfleirqs vrdmvstsdk ylrefgvkin peptqvvgri
      481 ldppslvfen nsmckprdgt wdlrgqrfyk amsmtqwivl nvsrfaqkdc ldnfikmlir
      541 igqelgmria qplavitfdt nrkpmrtvlt eqrkqrpqle mvvavitkat nyaeikqvae
      601 telslrtqci ldnnvvqkcn aaliqnlcqk inakmgginn slliqekpkl lhrpviviga
      661 dvshpspgdk irpsiaacvg sldsvpskfh atirvqieds kakarveiik dlkdmikell
      721 lvfyrttghk plrivfyrdg vsegqflevr nhevsairla caemcptety eppltfivvq
      781 krhhtrfmpa ndrdgvgkfr nvppgttvds vvthpldfdf flcshfgiqg tsrpahyyiv
      841 wddsnfsadd lqklsfylch tysrcarsvs ipapvyyahl aafrakhhia skletsgavs
      901 qssegggdav lttaqyveav kvlqelqasm yfv
//
LOCUS       XP_077505988             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115463 [Amblyomma americanum].
ACCESSION   XP_077505988
VERSION     XP_077505988.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649862.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144115463"
                     /calculated_mol_wt=17528
     Region          1..>60
                     /region_name="SanA"
                     /note="Uncharacterized periplasmic protein SanA, affects
                     membrane permeability for vancomycin [Cell
                     wall/membrane/envelope biogenesis]; COG2949"
                     /db_xref="CDD:442192"
     CDS             1..158
                     /gene="LOC144115463"
                     /coded_by="XM_077649862.1:193..669"
                     /db_xref="GeneID:144115463"
ORIGIN      
        1 mrkalstckk tlvaiwaahs ylapvlslqi vsmqtspcvs elisdlpptp mvvvlgtsif
       61 dcdctcivtl dgakcfegls flaavetlya ayfclnmkyn qevqatlefi qrqlvgmnpe
      121 egsrqlnrri tsvhpkvhsa lqqldklkkk wqdcmsri
//
LOCUS       XP_077505989             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115464 [Amblyomma americanum].
ACCESSION   XP_077505989
VERSION     XP_077505989.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649863.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144115464"
                     /calculated_mol_wt=17528
     Region          1..>60
                     /region_name="SanA"
                     /note="Uncharacterized periplasmic protein SanA, affects
                     membrane permeability for vancomycin [Cell
                     wall/membrane/envelope biogenesis]; COG2949"
                     /db_xref="CDD:442192"
     CDS             1..158
                     /gene="LOC144115464"
                     /coded_by="XM_077649863.1:231..707"
                     /db_xref="GeneID:144115464"
ORIGIN      
        1 mrkalstckk tlvaiwaahs ylapvlslqi vsmqtspcvs elisdlpptp mvvvlgtsif
       61 dcdctcivtl dgakcfegls flaavetlya ayfclnmkyn qevqatlefi qrqlvgmnpe
      121 egsrqlnrri tsvhpkvhsa lqqldklkkk wqdcmsri
//
LOCUS       XP_077505990             170 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-1-like [Amblyomma americanum].
ACCESSION   XP_077505990
VERSION     XP_077505990.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649864.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..170
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..170
                     /product="protein argonaute-1-like"
                     /calculated_mol_wt=18851
     Region          <7..126
                     /region_name="Piwi-like"
                     /note="PIWI domain. Domain found in proteins involved in
                     RNA silencing. RNA silencing refers to a group of related
                     gene-silencing mechanisms mediated by short RNA molecules,
                     including siRNAs, miRNAs, and heterochromatin-related
                     guide RNAs. The central component...; cl00628"
                     /db_xref="CDD:412485"
     CDS             1..170
                     /gene="LOC144115465"
                     /coded_by="XM_077649864.1:23..535"
                     /db_xref="GeneID:144115465"
ORIGIN      
        1 mcptetyepp ltfivvqkrh htrfmpandr dgvgkfrnvp pgttvdsvvt hpldfdfflc
       61 shfgiqgtsr pahyyivwdd snfsaddlqk lsfylchtys rcarsvsipa pvyyahlaaf
      121 rakhhiaskl etsgavsqss egggdavltt aqyveavkvl qelqasmyfv
//
LOCUS       XP_077505991             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115467 [Amblyomma americanum].
ACCESSION   XP_077505991
VERSION     XP_077505991.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649865.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144115467"
                     /calculated_mol_wt=17528
     Region          1..>60
                     /region_name="SanA"
                     /note="Uncharacterized periplasmic protein SanA, affects
                     membrane permeability for vancomycin [Cell
                     wall/membrane/envelope biogenesis]; COG2949"
                     /db_xref="CDD:442192"
     CDS             1..158
                     /gene="LOC144115467"
                     /coded_by="XM_077649865.1:233..709"
                     /db_xref="GeneID:144115467"
ORIGIN      
        1 mrkalstckk tlvaiwaahs ylapvlslqi vsmqtspcvs elisdlpptp mvvvlgtsif
       61 dcdctcivtl dgakcfegls flaavetlya ayfclnmkyn qevqatlefi qrqlvgmnpe
      121 egsrqlnrri tsvhpkvhsa lqqldklkkk wqdcmsri
//
LOCUS       XP_077505992             648 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077505992
VERSION     XP_077505992.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649866.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..648
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=73463
     Region          16..130
                     /region_name="PAZ_argonaute_like"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; cd02846"
                     /db_xref="CDD:239212"
     Site            order(68,83,92,96,113,120,122)
                     /site_type="other"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]"
                     /db_xref="CDD:239212"
     Region          175..604
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(307,311,323..326,329,338,341,345,349)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(376,378,454,594)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..648
                     /gene="LOC144115468"
                     /coded_by="XM_077649866.1:1002..2948"
                     /db_xref="GeneID:144115468"
ORIGIN      
        1 mlnvdmsata fyeslplvdf mcrffsdsrr vltpadlrsl rdnqyarlnr elkglrvkvt
       61 hlpyprkykv vkitreaakd iyfesegsqi svadyfqsry rrlsypnfpc vqsgspthpv
      121 yiplevcela egqhcrkkle esqtaemmkr takppakrfl eirqsvrdmv stsdkylref
      181 gvkinpeptq vvgrildpps lvfennsmck prdgtwdlrg qrfykamsmt qwivlnvsrf
      241 aqkdcldnfi kmlirigqel gmriaqplav itfdtnrkpm rtvlteqckq rpqlemvvav
      301 itkatnyaei kqvaetelsl rtqcildnnv vqkcnaaliq nlcqkinakm gginnslliq
      361 ekpkllhrpv ivigadvshp spgdkirpsi aacvgsldsv pskfhatirv qiedskakar
      421 veiikdlkdm ikelllvfyr ttghkplriv fyrdgvsegq flevrnhevs airlacaemc
      481 ptetyepplt fivvqkrhht rfmpandrdg vgkfrnvppg ttvdsvvthp ldfdfflcsh
      541 fgiqgtsrpa hyyivwddsn fsaddlqkls fylchtysrc arsvsipapv yyahlaafra
      601 khhiasklet sgavsqsseg ggdavlttaq yveavkvlqe lqasmyfv
//
LOCUS       XP_077505994            1012 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505994
VERSION     XP_077505994.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649868.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1012
                     /product="protein argonaute-2-like isoform X1"
                     /calculated_mol_wt=112526
     Region          <14..>90
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     Region          151..1011
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          539..968
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(671,675,687..690,693,702,705,709,713)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(740,742,818,958)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..1012
                     /gene="LOC144115469"
                     /coded_by="XM_077649868.1:422..3460"
                     /db_xref="GeneID:144115469"
ORIGIN      
        1 mgkgerkdkk kndsaggppa vpsasggnqa tapapspagt prdlfaqvlq qgaapspqpr
       61 avsrgpapaa vpraqptaqp sasrpsygap aaagdanvaa ssprpasaaa avsrsqpaaa
      121 alppsppcts pvavteqtpe agdqatpggd vsikelertl pshfprrpah gqlgrtiqll
      181 anhfsieipt gsvyhydvdi ssetaketkv peqknyrcls tkinriviel lvkkyrqdla
      241 ncipafdgrk nlytrrqlnf rertftvdie edqrsqkfii kiqyaatvnl dalhgvfqkr
      301 vqtvpqevlq aidivlrhsp sinlapvgrs ffrppgpneh ndlgggrevw fgyyasvrpa
      361 qwkpmlnvdm satafyeslp lvdfmcrffs dsrrvltpad lrslrdnqya rlnrelkglr
      421 vkvthlpypr kykvvkitre aakdiyfese gsqisvadyf qsryrrlsyp nfpcvqsgsp
      481 thpvyiplev celaegqhcr kkleesqtae mmkrtakppa krfleirqsv rdmvstsdky
      541 lrefgvkinp eptqvvgril dppslvfenn smckprdgtw dlrgqrfyka msmtqwivln
      601 vsrfaqkdcl dnfikmliri gqelgmriaq plavitfdtn rkpmrtvlte qrkqrpqlem
      661 vvavitkatn yaeikqvaet elslrtqcil dnnvvqkcna aliqnlcqki nakmgginns
      721 lliqekpkll hrpvivigad vshpspgdki rpsiaacvgs ldsvpskfha tirvqiedsk
      781 akarveiikd lkdmikelll vfyrttghkp lrivfyrdgv segqflevrn hevsairlac
      841 aemcptetye ppltfivvqk rhhtrfmpan drdgvgkfrn vppgttvdsv vthpldfdff
      901 lcshfgiqgt srpahyyivw ddsnfsaddl qklsfylcht ysrcarsvsi papvyyahla
      961 afrakhhias kletsgavsq ssegggdavl ttaqyveavk vlqelqasmy fv
//
LOCUS       XP_077505995             933 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505995
VERSION     XP_077505995.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649869.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..933
                     /product="protein argonaute-2-like isoform X2"
                     /calculated_mol_wt=106044
     Region          58..932
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          460..889
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(592,596,608..611,614,623,626,630,634)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(661,663,739,879)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..933
                     /gene="LOC144115469"
                     /coded_by="XM_077649869.1:42..2843"
                     /db_xref="GeneID:144115469"
ORIGIN      
        1 mvhlnggggp qtadrttefl wqcalpqlra itkkmhspvh tygevslpeq vqdvlrrgpk
       61 faveprqsas ellamelert lpshfprrpa hgqlgrtiql lanhfsieip tgsvyhydvd
      121 issetaketk vpeqknyrcl stkinrivie llvkkyrqdl ancipafdgr knlytrrqln
      181 frertftvdi eedqrsqkfi ikiqyaatvn ldalhgvfqk rvqtvpqevl qaidivlrhs
      241 psinlapvgr sffrppgpne hndlgggrev wfgyyasvrp aqwkpmlnvd msatafyesl
      301 plvdfmcrff sdsrrvltpa dlrslrdnqy arlnrelkgl rvkvthlpyp rkykvvkitr
      361 eaakdiyfes egsqisvady fqsryrrlsy pnfpcvqsgs pthpvyiple vcelaegqhc
      421 rkkleesqta emmkrtakpp akrfleirqs vrdmvstsdk ylrefgvkin peptqvvgri
      481 ldppslvfen nsmckprdgt wdlrgqrfyk amsmtqwivl nvsrfaqkdc ldnfikmlir
      541 igqelgmria qplavitfdt nrkpmrtvlt eqrkqrpqle mvvavitkat nyaeikqvae
      601 telslrtqci ldnnvvqkcn aaliqnlcqk inakmgginn slliqekpkl lhrpviviga
      661 dvshpspgdk irpsiaacvg sldsvpskfh atirvqieds kakarveiik dlkdmikell
      721 lvfyrttghk plrivfyrdg vsegqflevr nhevsairla caemcptety eppltfivvq
      781 krhhtrfmpa ndrdgvgkfr nvppgttvds vvthpldfdf flcshfgiqg tsrpahyyiv
      841 wddsnfsadd lqklsfylch tysrcarsvs ipapvyyahl aafrakhhia skletsgavs
      901 qssegggdav lttaqyveav kvlqelqasm yfv
//
LOCUS       XP_077505996            1012 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077505996
VERSION     XP_077505996.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649870.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1012
                     /product="protein argonaute-2-like isoform X1"
                     /calculated_mol_wt=112556
     Region          <14..>90
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     Region          151..1011
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          539..968
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(671,675,687..690,693,702,705,709,713)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(740,742,818,958)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..1012
                     /gene="LOC144115470"
                     /coded_by="XM_077649870.1:62..3100"
                     /db_xref="GeneID:144115470"
ORIGIN      
        1 mgkgerkdkk kndsaggppa vpsasggnqa tapapspagt prdlfaqvlq qgaapspqpr
       61 avsrgpapaa vpraqptaqp sasrpsygap aaagdanvaa ssprpasaaa avsrsqpaaa
      121 alppsppcts pvavteqtpe agdqatpggd vsikelertl pshfprrpah gqlsrtiqll
      181 anhfsieipt gsvyhydvdi ssetaketkv peqknyrcls tkinriviel lvkkyrqdla
      241 ncipafdgrk nlytrrqlnf rertftvdie edqrsqkfii kiqyaatvnl dalhgvfqkr
      301 vqtvpqevlq aidivlrhsp sinlapvgrs ffrppgpneh ndlgggrevw fgyyasvrpa
      361 qwkpmlnvdm satafyeslp lvdfmcrffs dsrrvltpad lrslrdnqya rlnrelkglr
      421 vkvthlpypr kykvvkitre aakdiyfese gsqisvadyf qsryrrlsyp nfpcvqsgsp
      481 thpvyiplev celaegqhcr kkleesqtae mmkrtakppa krfleirqsv rdmvstsdky
      541 lrefgvkinp eptqvvgril dppslvfenn smckprdgtw dlrgqrfyka msmtqwivln
      601 vsrfaqkdcl dnfikmliri gqelgmriaq plavitfdtn rkpmrtvlte qrkqrpqlem
      661 vvavitkatn yaeikqvaet elslrtqcil dnnvvqkcna aliqnlcqki nakmgginns
      721 lliqekpkll hrpvivigad vshpspgdki rpsiaacvgs ldsvpskfha tirvqiedsk
      781 akarveiikd lkdmikelll vfyrttghkp lrivfyrdgv segqflevrn hevsairlac
      841 aemcptetye ppltfivvqk rhhtrfmpan drdgvgkfrn vppgttvdsv vthpldfdff
      901 lcshfgiqgt srpahyyivw ddsnfsaddl qklsfylcht ysrcarsvsi papvyyahla
      961 afrakhhias kletsgavsq ssegggdavl ttaqyveavk vlqelqasmy fv
//
LOCUS       XP_077505997             909 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077505997
VERSION     XP_077505997.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649871.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..909
                     /product="protein argonaute-2-like isoform X2"
                     /calculated_mol_wt=103430
     Region          48..908
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          436..865
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(568,572,584..587,590,599,602,606,610)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(637,639,715,855)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..909
                     /gene="LOC144115470"
                     /coded_by="XM_077649871.1:146..2875"
                     /db_xref="GeneID:144115470"
ORIGIN      
        1 myvarcsnra dprswrpghs rrrcqyqaaa vgfdpgnags viehsnhrat aelertlpsh
       61 fprrpahgql srtiqllanh fsieiptgsv yhydvdisse taketkvpeq knyrclstki
      121 nriviellvk kyrqdlanci pafdgrknly trrqlnfrer tftvdieedq rsqkfiikiq
      181 yaatvnldal hgvfqkrvqt vpqevlqaid ivlrhspsin lapvgrsffr ppgpnehndl
      241 gggrevwfgy yasvrpaqwk pmlnvdmsat afyeslplvd fmcrffsdsr rvltpadlrs
      301 lrdnqyarln relkglrvkv thlpyprkyk vvkitreaak diyfesegsq isvadyfqsr
      361 yrrlsypnfp cvqsgspthp vyiplevcel aegqhcrkkl eesqtaemmk rtakppakrf
      421 leirqsvrdm vstsdkylre fgvkinpept qvvgrildpp slvfennsmc kprdgtwdlr
      481 gqrfykamsm tqwivlnvsr faqkdcldnf ikmlirigqe lgmriaqpla vitfdtnrkp
      541 mrtvlteqrk qrpqlemvva vitkatnyae ikqvaetels lrtqcildnn vvqkcnaali
      601 qnlcqkinak mgginnslli qekpkllhrp vivigadvsh pspgdkirps iaacvgslds
      661 vpskfhatir vqiedskaka rveiikdlkd mikelllvfy rttghkplri vfyrdgvseg
      721 qflevrnhev sairlacaem cptetyeppl tfivvqkrhh trfmpandrd gvgkfrnvpp
      781 gttvdsvvth pldfdfflcs hfgiqgtsrp ahyyivwdds nfsaddlqkl sfylchtysr
      841 carsvsipap vyyahlaafr akhhiaskle tsgavsqsse gggdavltta qyveavkvlq
      901 elqasmyfv
//
LOCUS       XP_077505998            1012 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077505998
VERSION     XP_077505998.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649872.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1012
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=112508
     Region          <14..>90
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     Region          151..1011
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          539..968
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(671,675,687..690,693,702,705,709,713)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(740,742,818,958)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..1012
                     /gene="LOC144115471"
                     /coded_by="XM_077649872.1:143..3181"
                     /db_xref="GeneID:144115471"
ORIGIN      
        1 mgkgerkdkk kndsaggppa vpsasggnqa tapapspagt prdlfaqvlq qgaapspqpr
       61 avsrgpapaa vpraqptaqp sasrpsygap aaagdanvaa ssprpasaaa avsrsqpaaa
      121 alppsppcts pvavteqtpe agdqatpggd vsikelertl pshfprrpah gqlgrtiqll
      181 anhfsieipt gsvyhydvdi ssetaketkv peqknyrcls tkinriviel lvkkyrqdla
      241 ncipafdgrk nlytrrqlnf rertftvdie edqrsqkfii kiqyaatvnl dalhgvfqkr
      301 vqtvpqevlq aidivlrhsp sinlapvgrs ffrppgpneh ndlgggrevw fgyyasvrpa
      361 qwkpmlnvdm satafyeslp lvdfmcrffs dsrrvltpad lrslrdnqya rlnrelkglr
      421 vkvthlpypr kykvvkitre aakdiyfese gsqisvadyf qsryrrlsyp nfpcvqsgsp
      481 thpvyiplev celaegqhcr kkleesqtae mikrtakppa krfleirqsv rdmvstsdky
      541 lrefgvkinp eptqvvgril dppslvfenn smckprdgtw dlrgqrfyka msmtqwivln
      601 vsrfaqkdcl dnfikmliri gqelgmriaq plavitfdtn rkpmrtvlte qrkqrpqlem
      661 vvavitkatn yaeikqvaet elslrtqcil dnnvvqkcna aliqnlcqki nakmgginns
      721 lliqekpkll hrpvivigad vshpspgdki rpsiaacvgs ldsvpskfha tirvqiedsk
      781 akarveiikd lkdmikelll vfyrttghkp lrivfyrdgv segqflevrn hevsairlac
      841 aemcptetye ppltfivvqk rhhtrfmpan drdgvgkfrn vppgttvdsv vthpldfdff
      901 lcshfgiqgt srpahyyivw ddsnfsaddl qklsfylcht ysrcarsvsi papvyyahla
      961 afrakhhias kletsgavsq ssegggdavl ttaqyveavk vlqelqasmy fv
//
LOCUS       XP_077505999            1012 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077505999
VERSION     XP_077505999.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649873.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1012
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=112508
     Region          <14..>90
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     Region          151..1011
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          539..968
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(671,675,687..690,693,702,705,709,713)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(740,742,818,958)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..1012
                     /gene="LOC144115472"
                     /coded_by="XM_077649873.1:143..3181"
                     /db_xref="GeneID:144115472"
ORIGIN      
        1 mgkgerkdkk kndsaggppa vpsasggnqa tapapspagt prdlfaqvlq qgaapspqpr
       61 avsrgpapaa vpraqptaqp sasrpsygap aaagdanvaa ssprpasaaa avsrsqpaaa
      121 alppsppcts pvavteqtpe agdqatpggd vsikelertl pshfprrpah gqlgrtiqll
      181 anhfsieipt gsvyhydvdi ssetaketkv peqknyrcls tkinriviel lvkkyrqdla
      241 ncipafdgrk nlytrrqlnf rertftvdie edqrsqkfii kiqyaatvnl dalhgvfqkr
      301 vqtvpqevlq aidivlrhsp sinlapvgrs ffrppgpneh ndlgggrevw fgyyasvrpa
      361 qwkpmlnvdm satafyeslp lvdfmcrffs dsrrvltpad lrslrdnqya rlnrelkglr
      421 vkvthlpypr kykvvkitre aakdiyfese gsqisvadyf qsryrrlsyp nfpcvqsgsp
      481 thpvyiplev celaegqhcr kkleesqtae mikrtakppa krfleirqsv rdmvstsdky
      541 lrefgvkinp eptqvvgril dppslvfenn smckprdgtw dlrgqrfyka msmtqwivln
      601 vsrfaqkdcl dnfikmliri gqelgmriaq plavitfdtn rkpmrtvlte qrkqrpqlem
      661 vvavitkatn yaeikqvaet elslrtqcil dnnvvqkcna aliqnlcqki nakmgginns
      721 lliqekpkll hrpvivigad vshpspgdki rpsiaacvgs ldsvpskfha tirvqiedsk
      781 akarveiikd lkdmikelll vfyrttghkp lrivfyrdgv segqflevrn hevsairlac
      841 aemcptetye ppltfivvqk rhhtrfmpan drdgvgkfrn vppgttvdsv vthpldfdff
      901 lcshfgiqgt srpahyyivw ddsnfsaddl qklsfylcht ysrcarsvsi papvyyahla
      961 afrakhhias kletsgavsq ssegggdavl ttaqyveavk vlqelqasmy fv
//
LOCUS       XP_077506000             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115473 [Amblyomma americanum].
ACCESSION   XP_077506000
VERSION     XP_077506000.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649874.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144115473"
                     /calculated_mol_wt=17528
     Region          1..>60
                     /region_name="SanA"
                     /note="Uncharacterized periplasmic protein SanA, affects
                     membrane permeability for vancomycin [Cell
                     wall/membrane/envelope biogenesis]; COG2949"
                     /db_xref="CDD:442192"
     CDS             1..158
                     /gene="LOC144115473"
                     /coded_by="XM_077649874.1:254..730"
                     /db_xref="GeneID:144115473"
ORIGIN      
        1 mrkalstckk tlvaiwaahs ylapvlslqi vsmqtspcvs elisdlpptp mvvvlgtsif
       61 dcdctcivtl dgakcfegls flaavetlya ayfclnmkyn qevqatlefi qrqlvgmnpe
      121 egsrqlnrri tsvhpkvhsa lqqldklkkk wqdcmsri
//
LOCUS       XP_077506001            1016 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077506001
VERSION     XP_077506001.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649875.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1016
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=112906
     Region          <14..>94
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     Region          155..1015
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          543..972
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(675,679,691..694,697,706,709,713,717)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(744,746,822,962)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..1016
                     /gene="LOC144115474"
                     /coded_by="XM_077649875.1:641..3691"
                     /db_xref="GeneID:144115474"
ORIGIN      
        1 mgkgerkdkk kndsaggppa vpsasggnqa tapapspagt prdlfaqvlq qgaapspqpr
       61 avsrgpapaa vpraqptaqp taqpsasrps ygapaaagda nvaassprpa saaaavsrsq
      121 paaaalppsp pctspvavte qtpeagdqat pggdvsikel ertlpshfpr rpahgqlgrt
      181 iqllanhfsi eiptgsvyhy dvdissetak etkvpeqkny rclstkinri viellvkkyr
      241 qdlancipaf dgrknlytrr qlnfrertft vdieedqrsq kfiikiqyaa tvnldalhgv
      301 fqkrvqtvpq evlqaidivl rhspsinlap vgrsffrppg pnehndlggg revwfgyyas
      361 vrpaqwkpml nvdmsatafy eslplvdfmc rffsdsrrvl tpadlrslrd nqyarlnrel
      421 kglrvkvthl pyprkykvvk itreaakdiy fesegsqisv adyfqsryrr lsypnfpcvq
      481 sgspthpvyi plevcelaeg qhcrkklees qtaemikrta kppakrflei rqsvrdmvst
      541 sdkylrefgv kinpeptqvv grildppslv fennsmckpr dgtwdlrgqr fykamsmtqw
      601 ivlnvsrfaq kdcldnfikm lirigqelgm riaqplavit fdtnrkpmrt vlteqrkqrp
      661 qlemvvavit katnyaeikq vaetelslrt qcildnnvvq kcnaaliqnl cqkinakmgg
      721 innslliqek pkllhrpviv igadvshpsp gdkirpsiaa cvgsldsvps kfhatirvqi
      781 edskakarve iikdlkdmik elllvfyrtt ghkplrivfy rdgvsegqfl evrnhevsai
      841 rlacaemcpt etyeppltfi vvqkrhhtrf mpandrdgvg kfrnvppgtt vdsvvthpld
      901 fdfflcshfg iqgtsrpahy yivwddsnfs addlqklsfy lchtysrcar svsipapvyy
      961 ahlaafrakh hiaskletsg avsqsseggg davlttaqyv eavkvlqelq asmyfv
//
LOCUS       XP_077506002             502 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077506002
VERSION     XP_077506002.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649876.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..502
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=56511
     Region          29..458
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(161,165,177..180,183,192,195,199,203)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(230,232,308,448)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..502
                     /gene="LOC144115475"
                     /coded_by="XM_077649876.1:25..1533"
                     /db_xref="GeneID:144115475"
ORIGIN      
        1 mikrtakppa krfleirqsv rdmvstsdky lrefgvkinp eptqvvgril dppslvfenn
       61 smckprdgtw dlrgqrfyka msmtqwivln vsrfaqkdcl dnfikmliri gqelgmriaq
      121 plavitfdtn rkpmrtvlte qrkqrpqlem vvavitkatn yaeikqvaet elslrtqcil
      181 dnnvvqkcna aliqnlcqki nakmgginns lliqekpkll hrpvivigad vshpspgdki
      241 rpsiaacvgs ldsvpskfha tirvqiedsk akarveiikd lkdmikelll vfyrttghkp
      301 lrivfyrdgv segqflevrn hevsairlac aemcptetye ppltfivvqk rhhtrfmpan
      361 drdgvgkfrn vppgttvdsv vthpldfdff lcshfgiqgt srpahyyivw ddsnfsaddl
      421 qklsfylcht ysrcarsvsi papvyyahla afrakhhias kletsgavsq ssegggdavl
      481 ttaqyveavk vlqelqasmy fv
//
LOCUS       XP_077506003             158 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115476 [Amblyomma americanum].
ACCESSION   XP_077506003
VERSION     XP_077506003.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649877.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..158
                     /product="uncharacterized protein LOC144115476"
                     /calculated_mol_wt=17528
     Region          1..>60
                     /region_name="SanA"
                     /note="Uncharacterized periplasmic protein SanA, affects
                     membrane permeability for vancomycin [Cell
                     wall/membrane/envelope biogenesis]; COG2949"
                     /db_xref="CDD:442192"
     CDS             1..158
                     /gene="LOC144115476"
                     /coded_by="XM_077649877.1:245..721"
                     /db_xref="GeneID:144115476"
ORIGIN      
        1 mrkalstckk tlvaiwaahs ylapvlslqi vsmqtspcvs elisdlpptp mvvvlgtsif
       61 dcdctcivtl dgakcfegls flaavetlya ayfclnmkyn qevqatlefi qrqlvgmnpe
      121 egsrqlnrri tsvhpkvhsa lqqldklkkk wqdcmsri
//
LOCUS       XP_077506004            1016 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077506004
VERSION     XP_077506004.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649878.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1016
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=112825
     Region          <14..>94
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     Region          155..1015
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          543..972
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(675,679,691..694,697,706,709,713,717)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(744,746,822,962)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..1016
                     /gene="LOC144115477"
                     /coded_by="XM_077649878.1:59..3109"
                     /db_xref="GeneID:144115477"
ORIGIN      
        1 mgkgerkdkk kndsaggppa vpsasggnqa tapapspagt prdlfaqvlq qgaapspqpr
       61 avsrgpapaa vpraqptaqp taqpsasrps ygapaaagda nvaassprpa saaaavsrsq
      121 paaaalppsp pctspvavte qtpeagdqat pggdvsikel ertlpshfpr rpahgqlgrt
      181 iqllanhfsi eiptgsvyhy dvdissetak etkvpeqkny rclstkinri viellvkkyr
      241 qdlancipaf dgrknlytrr qlnfrertft vdieedqrsq kfiikiqyaa tvnldalhgv
      301 fqkrvqtvpq evlqaidivl rhspsinlap vgrsffrppg pnehndlggg revwfgyyas
      361 vrpaqwkpml nvdmsatafy eslplvdfmc rffsdsrrvl tpadlrslrd nqyarlnrel
      421 kglcvkvthl pyprkykvvk itreaakdiy fesegsqisv adyfqsryrr lsypnfpcvq
      481 sgspthpvyi plevcelaeg qhcrkklees qtaemikrta kppakrflei rqsvrdmvst
      541 sdkylrefgv kinpeptqvv grildppslv fennsmckpr dgtwdlrgqr fykamsmtqw
      601 ivlnvsrfaq kdcldnfikm lirigqelgm riaqplavit fdtnrkpmrt vlteqrkqrp
      661 qlemvvavit katnyaeikq vaetelslrt qcildnnvvq kcnaaliqnl cqkinakmgg
      721 innslliqek pkllhrpviv igadvshpsp gdkirpsiaa cvgsldsvps kfhatirvqi
      781 edskakarve iikdlkdmik elllvfyrtt ghkplrivfy rdgvsegqfl evrnhevsai
      841 rlacaemcpt etyeppltfi vvqkrhhtrf mpandrdgvg kfrnvppgtt vdsvvthpld
      901 fdfflcshfg iqgtsqpahy yivwddsnfs addlqklsfy lchtysrcar svsipapvyy
      961 ahlaafrakh hiaskletsg avsqsseggg davlttaqyv eavkvlqelq asmyfv
//
LOCUS       XP_077506005             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115479 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506005
VERSION     XP_077506005.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649879.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144115479 isoform X1"
                     /calculated_mol_wt=15193
     CDS             1..133
                     /gene="LOC144115479"
                     /coded_by="XM_077649879.1:346..747"
                     /db_xref="GeneID:144115479"
ORIGIN      
        1 mkrigyhsvk eqdkaerhkc svqlqsafht plfanqlady ttaaathhil swrgsrmsnl
       61 tkrngsfpll lpgsfqrral phdamnddir qwmkneatrs srrriirsrk ystaltastf
      121 pvqlsrpwtr gvs
//
LOCUS       XP_077506006             120 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115479 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506006
VERSION     XP_077506006.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649880.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..120
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..120
                     /product="uncharacterized protein LOC144115479 isoform X2"
                     /calculated_mol_wt=13113
     CDS             1..120
                     /gene="LOC144115479"
                     /coded_by="XM_077649880.1:346..708"
                     /db_xref="GeneID:144115479"
ORIGIN      
        1 mksgcadasg vlerhceadd nhdarvclqn krervsstnd pgtagaavqw efstsanaks
       61 khclsdgplk fhqcgyeelq eadhqeqeif dcfdrfnvpc aavtpldtrs llktnnsiev
//
LOCUS       XP_077506007              95 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115479 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077506007
VERSION     XP_077506007.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649881.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..95
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..95
                     /product="uncharacterized protein LOC144115479 isoform X3"
                     /calculated_mol_wt=10273
     CDS             1..95
                     /gene="LOC144115479"
                     /coded_by="XM_077649881.1:346..633"
                     /db_xref="GeneID:144115479"
ORIGIN      
        1 mkrigyhsvk eqdkaerhkc svqlqsafht plfanqlady ttaaathhil swrgsrmsnl
       61 tkrngsfpll lpgsfqrlrg apgggssgag nirll
//
LOCUS       XP_077506008              65 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115479 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077506008
VERSION     XP_077506008.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649882.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..65
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..65
                     /product="uncharacterized protein LOC144115479 isoform X4"
                     /calculated_mol_wt=7524
     CDS             1..65
                     /gene="LOC144115479"
                     /coded_by="XM_077649882.1:346..543"
                     /db_xref="GeneID:144115479"
ORIGIN      
        1 mkrigyhsvk eqdkaerhkc yeelqeadhq eqeifdcfdr fnvpcaavtp ldtrsllktn
       61 nsiev
//
LOCUS       XP_077506009             801 aa            linear   INV 25-NOV-2025
DEFINITION  polycystin-2-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077506009
VERSION     XP_077506009.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649883.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..801
                     /product="polycystin-2-like isoform X1"
                     /calculated_mol_wt=91213
     Region          156..345
                     /region_name="Polycystin_dom"
                     /note="Polycystin domain; pfam20519"
                     /db_xref="CDD:466668"
     Region          346..570
                     /region_name="PKD_channel"
                     /note="Polycystin cation channel; pfam08016"
                     /db_xref="CDD:462341"
     Region          606..665
                     /region_name="EF-hand_7"
                     /note="EF-hand domain pair; pfam13499"
                     /db_xref="CDD:463900"
     CDS             1..801
                     /gene="LOC144115482"
                     /coded_by="XM_077649883.1:160..2565"
                     /db_xref="GeneID:144115482"
ORIGIN      
        1 msnpasnltd srpnsgrrpr ssvrptsagr pawntaqltr eesrdleafg andqfeivdd
       61 sevvtvpeqk gclasclrgl rglwstrqlg gakadrewyi rttlrelliy vvflvvlcil
      121 tfgmmsptmy yqtkvmsdlf ldstfkdnts nlrgstsldn fwsfvedvml galywenwyn
      181 nqstladdrn ilyenrllgs prirqlrvrn dscnvhsdfk kaitqcfdsy sphfeekgpf
      241 glmngsawty htekelkgad hwgllssysg agyyadlgit keaatqvmad lkenlwigra
      301 travfldftv ynanvnlfcv iklvfefpat ggmipswsfr tvkllryvsv sdyfimacef
      361 fftlfilyyi veevleiksn rtsyfksvwn ildilvilis lvcigfsiyr tvmvnkllag
      421 lldkpdefad fgrlgfyqtq fnnavalavf fawikvfkyi sfnktmtqls stlsrcskdv
      481 agfsimffiv ffafaqlgyl lfgtqiqgfs sfvnavftll rlilgdfdfe emeaanrvlg
      541 piyflsyvff vffvllnmfl aiindtyaev kseisqqkne felvdyfkkg ynnilgklgk
      601 rdqivdiqna lkmadtnhdn kltfdevrkt lkaqnfsdme iemlfskydv dgnrelneqe
      661 tkemmahleg qkvlldeeiq rektsgndng gggggngggg eggvspdefn iltrrvdrme
      721 hsigsivski davlvkmeam ekakasrrdn mskildtise negldekgkr qqmeklvree
      781 lqhwdsdasl htprrersnt p
//
LOCUS       XP_077506010             678 aa            linear   INV 25-NOV-2025
DEFINITION  polycystin-2-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077506010
VERSION     XP_077506010.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649884.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..678
                     /product="polycystin-2-like isoform X2"
                     /calculated_mol_wt=77517
     Region          33..222
                     /region_name="Polycystin_dom"
                     /note="Polycystin domain; pfam20519"
                     /db_xref="CDD:466668"
     Region          223..447
                     /region_name="PKD_channel"
                     /note="Polycystin cation channel; pfam08016"
                     /db_xref="CDD:462341"
     Region          483..542
                     /region_name="EF-hand_7"
                     /note="EF-hand domain pair; pfam13499"
                     /db_xref="CDD:463900"
     Region          581..615
                     /region_name="Fer4_24"
                     /note="Ferredoxin I 4Fe-4S cluster domain; pfam18109"
                     /db_xref="CDD:436280"
     CDS             1..678
                     /gene="LOC144115482"
                     /coded_by="XM_077649884.1:428..2464"
                     /db_xref="GeneID:144115482"
ORIGIN      
        1 mmsptmyyqt kvmsdlflds tfkdntsnlr gstsldnfws fvedvmlgal ywenwynnqs
       61 tladdrnily enrllgspri rqlrvrndsc nvhsdfkkai tqcfdsysph feekgpfglm
      121 ngsawtyhte kelkgadhwg llssysgagy yadlgitkea atqvmadlke nlwigratra
      181 vfldftvyna nvnlfcvikl vfefpatggm ipswsfrtvk llryvsvsdy fimaceffft
      241 lfilyyivee vleiksnrts yfksvwnild ilvilislvc igfsiyrtvm vnkllaglld
      301 kpdefadfgr lgfyqtqfnn avalavffaw ikvfkyisfn ktmtqlsstl srcskdvagf
      361 simffivffa faqlgyllfg tqiqgfssfv navftllrli lgdfdfeeme aanrvlgpiy
      421 flsyvffvff vllnmflaii ndtyaevkse isqqknefel vdyfkkgynn ilgklgkrdq
      481 ivdiqnalkm adtnhdnklt fdevrktlka qnfsdmeiem lfskydvdgn relneqetke
      541 mmahlegqkv lldeeiqrek tsgndngggg ggnggggegg vspdefnilt rrvdrmehsi
      601 gsivskidav lvkmeameka kasrrdnmsk ildtiseneg ldekgkrqqm eklvreelqh
      661 wdsdaslhtp rrersntp
//
LOCUS       XP_077506011            1170 aa            linear   INV 25-NOV-2025
DEFINITION  slowpoke 2 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077506011
VERSION     XP_077506011.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649885.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1170
                     /product="slowpoke 2 isoform X1"
                     /calculated_mol_wt=132336
     Region          <156..298
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          <254..297
                     /region_name="Ion_trans_2"
                     /note="Ion channel; pfam07885"
                     /db_xref="CDD:462301"
     Region          <256..>348
                     /region_name="PRK10537"
                     /note="voltage-gated potassium channel protein"
                     /db_xref="CDD:236711"
     Region          460..554
                     /region_name="BK_channel_a"
                     /note="Calcium-activated BK potassium channel alpha
                     subunit; pfam03493"
                     /db_xref="CDD:460947"
     CDS             1..1170
                     /gene="SLO2"
                     /coded_by="XM_077649885.1:136..3648"
                     /db_xref="GeneID:144115483"
ORIGIN      
        1 mnaptpggva plppryrfrd lilgdfaygd dgtrvrvefy vnentlkerl hlyfiknqrs
       61 slrirvirlv vkivacllyv grvaldrgpr yatcyqcplp fenrsrytde avrngainwd
      121 aifwverpfa lwciqtilai isfseaclvv yltykgniwr qllsfrflle ivnnvpfivt
      181 ifyaplrnvf ipvflncwla kfvlenmfnd lhralqksqs alsqrllilt atllclifts
      241 vcgfqhfqra gvehvdlfes fyfvvvtlst vgygdfkpdv wpsqlfmvgm icaalivlpt
      301 qfeqlaytwm erqklggtys mhraqserhv vvcsttlrad tvmdflnefy ahpllqdyyv
      361 vllspcevda tmkmilqipm waqrviyiqg salkdtdltr armnaaeacf mlaarnyadl
      421 aaadqhtilr swavrdfapn vpqyiqifrp enkvhvafae hvvcedefky allannclcp
      481 gastlvtlll htsrgqegqt saeewhrlyg kcsgneiyhi rladsrffge yegksftyas
      541 fhshrkygva lvgvqtdvhg qwpvllnpgp ayimkgsdic fymnitkeen saflpaagdd
      601 akpqeaadka aadakaktes gksrfdfrsr kqhlevpkpe eargrrpsia pvpdlgqsde
      661 gtqldsedda lgieeekyle nswntpaees eivkgfppvt pyigvspklc yllrdkkplc
      721 cmqlaqqcdh cefhnakqyn wpnrcvilaa dfaskgiynf ivplrahfhs pnslrpivll
      781 lekkphaafl daiscfplvy wmqgsiddld dllraginla dsvvvvnkes tnsaeedyla
      841 dcntivavqt mfkffpsvri itelsqssnm rfmqfrafdt yalhlskmek vsllnqrerd
      901 rgshisymfr lpfaagnvfs asmldtllyq afvkdyvitf vrlllgvdqa pgsgflssmr
      961 itkddlwirt ygrlyqklcs ttceipigiy rtqsttnpeg stvsldlldd katfnenier
     1021 qeistlvrtr mqdlglpvqd ydevsdkrhs lsyviinpnc dlpleegdii flirpspisg
     1081 kriflkhgsv riktprpsrr enpssrgsgr sktpsfrlnf gahkadslpv qenggltppk
     1141 ppqetaieqs ecsvhvtaat psstgqgtiv
//
LOCUS       XP_077506012            1159 aa            linear   INV 25-NOV-2025
DEFINITION  slowpoke 2 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077506012
VERSION     XP_077506012.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649886.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1159
                     /product="slowpoke 2 isoform X2"
                     /calculated_mol_wt=131164
     Region          <144..286
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          <242..285
                     /region_name="Ion_trans_2"
                     /note="Ion channel; pfam07885"
                     /db_xref="CDD:462301"
     Region          <244..>336
                     /region_name="PRK10537"
                     /note="voltage-gated potassium channel protein"
                     /db_xref="CDD:236711"
     Region          448..542
                     /region_name="BK_channel_a"
                     /note="Calcium-activated BK potassium channel alpha
                     subunit; pfam03493"
                     /db_xref="CDD:460947"
     CDS             1..1159
                     /gene="SLO2"
                     /coded_by="XM_077649886.1:67..3546"
                     /db_xref="GeneID:144115483"
ORIGIN      
        1 msalwqalsl cvapvrreag fmvrvefyvn entlkerlhl yfiknqrssl rirvirlvvk
       61 ivacllyvgr valdrgprya tcyqcplpfe nrsrytdeav rngainwdai fwverpfalw
      121 ciqtilaiis fseaclvvyl tykgniwrql lsfrflleiv nnvpfivtif yaplrnvfip
      181 vflncwlakf vlenmfndlh ralqksqsal sqrlliltat llcliftsvc gfqhfqragv
      241 ehvdlfesfy fvvvtlstvg ygdfkpdvwp sqlfmvgmic aalivlptqf eqlaytwmer
      301 qklggtysmh raqserhvvv csttlradtv mdflnefyah pllqdyyvvl lspcevdatm
      361 kmilqipmwa qrviyiqgsa lkdtdltrar mnaaeacfml aarnyadlaa adqhtilrsw
      421 avrdfapnvp qyiqifrpen kvhvafaehv vcedefkyal lannclcpga stlvtlllht
      481 srgqegqtsa eewhrlygkc sgneiyhirl adsrffgeye gksftyasfh shrkygvalv
      541 gvqtdvhgqw pvllnpgpay imkgsdicfy mnitkeensa flpaagddak pqeaadkaaa
      601 dakaktesgk srfdfrsrkq hlevpkpeea rgrrpsiapv pdlgqsdegt qldseddalg
      661 ieeekylens wntpaeesei vkgfppvtpy igvspklcyl lrdkkplccm qlaqqcdhce
      721 fhnakqynwp nrcvilaadf askgiynfiv plrahfhspn slrpivllle kkphaaflda
      781 iscfplvywm qgsiddlddl lraginlads vvvvnkestn saeedyladc ntivavqtmf
      841 kffpsvriit elsqssnmrf mqfrafdtya lhlskmekvs llnqrerdrg shisymfrlp
      901 faagnvfsas mldtllyqaf vkdyvitfvr lllgvdqapg sgflssmrit kddlwirtyg
      961 rlyqklcstt ceipigiyrt qsttnpegst vsldllddka tfnenierqe istlvrtrmq
     1021 dlglpvqdyd evsdkrhsls yviinpncdl pleegdiifl irpspisgkr iflkhgsvri
     1081 ktprpsrren pssrgsgrsk tpsfrlnfga hkadslpvqe nggltppkpp qetaievqse
     1141 csvhvtaatp sstgqgtiv
//
LOCUS       XP_077506013             582 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115484 [Amblyomma americanum].
ACCESSION   XP_077506013
VERSION     XP_077506013.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649887.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..582
                     /product="uncharacterized protein LOC144115484"
                     /calculated_mol_wt=63293
     Region          222..574
                     /region_name="XK-related"
                     /note="XK-related protein; pfam09815"
                     /db_xref="CDD:462912"
     CDS             1..582
                     /gene="LOC144115484"
                     /coded_by="XM_077649887.1:648..2396"
                     /db_xref="GeneID:144115484"
ORIGIN      
        1 msygspcrct cgarsdarfq yrapprqqry trvklsrqar vaeqssivtt fsssgtwsli
       61 egvagddada fiellssrqr rdsvavdgdg ssteltqats igcatpasnv isgrsccssv
      121 stvrsasnel tsserlvvql fmdeadikek emaaaleaaa arehdedrss sgvevapneg
      181 espwpscnra eterarrrga rmgcgalvap stlaflvlta vlvsnallai vyyegrtwlc
      241 vttlglmhap slvlfvarvy svvvrrsike rfmkvvvlvw yavtlpllhc lplltlssvl
      301 fdkalmvmgd psdmrpfigr qeevafgdlv wvavlhafpq avlqlhmllf tegagflrge
      361 wpdytatlqm gsalsgalli acvatfysky vvrgrwcdee ttdgsttyet tttaalqdcd
      421 eedtvcgyml kfvawllias arvvagasll avsfgpavla lalahvpvvl lwslvcrsry
      481 swakffgdia lglithvfvl dyralnregl astmsavvfl catfventvc lvlaylvvgs
      541 epphytvllv lvgahyaama cgvflllchf rmrhrkaaey vf
//
LOCUS       XP_077506014             491 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115485 [Amblyomma americanum].
ACCESSION   XP_077506014
VERSION     XP_077506014.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649888.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..491
                     /product="uncharacterized protein LOC144115485"
                     /calculated_mol_wt=54115
     CDS             1..491
                     /gene="LOC144115485"
                     /coded_by="XM_077649888.1:480..1955"
                     /db_xref="GeneID:144115485"
ORIGIN      
        1 merndavskr ptdngvasve wldrfhrekr qrrsmrhcya agpkkcagav spaascamip
       61 rqdslasvce dppqqspqkt snqgrpdlll sgmfrplpdy rqpqdddaeq peltvtcpes
      121 rsapftlvls sayavvlhvc faasswwlvq kylaaglrmq agitlflaql sslplflvgv
      181 crligreqqp ssgcilllvl hvlgaglfnm vpllqlvlrl ldsivlyrtr gkqdqaallk
      241 rqdtitftnl mwvatlscfp qavfqaeqlf eaalqgqayt lgsswlqaan lscallltgf
      301 acvkfnnrha ktvftwptiq salrtaaeqa awlpllaarv lafavliaka lepthvfgif
      361 falslhfefr rdrasllagq prgfllsvaa nvaratvgmf fklrnwqepl aakpyivfai
      421 mllvqsfgcv flpflqqygf smsmdafray yaepvfvaci svhyallvlg tafllvwyrv
      481 egagtsgyga i
//
LOCUS       XP_077506015             150 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115486 [Amblyomma americanum].
ACCESSION   XP_077506015
VERSION     XP_077506015.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649889.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..150
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..150
                     /product="uncharacterized protein LOC144115486"
                     /calculated_mol_wt=17102
     CDS             1..150
                     /gene="LOC144115486"
                     /coded_by="XM_077649889.1:389..841"
                     /db_xref="GeneID:144115486"
ORIGIN      
        1 mfwtrltnll nhefggtlsa vqvenkwksl ersykrartk nntsghhrvp ceyeeelskv
       61 lekehhiqpp alletgasag nsdvaaedmn ssrdmeevpa smprrkrqrt ssnsvlaetl
      121 lkldaarakr heermakfdm lieamrlnkq
//
LOCUS       XP_077506016             899 aa            linear   INV 25-NOV-2025
DEFINITION  inturned planar cell polarity protein [Amblyomma americanum].
ACCESSION   XP_077506016
VERSION     XP_077506016.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649890.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..899
                     /product="inturned planar cell polarity protein"
                     /calculated_mol_wt=99706
     Region          <185..241
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cl49608"
                     /db_xref="CDD:483948"
     Region          482..588
                     /region_name="Intu_longin_2"
                     /note="Intu longin-like domain 2; pfam19032"
                     /db_xref="CDD:465951"
     Region          756..893
                     /region_name="Intu_longin_3"
                     /note="Intu longin-like domain 3; pfam19033"
                     /db_xref="CDD:436913"
     CDS             1..899
                     /gene="in"
                     /coded_by="XM_077649890.1:323..3022"
                     /db_xref="GeneID:144115487"
ORIGIN      
        1 mtslpqtlri lenshvnraf seevcdcdss satpdgcsek syepewaeni gprgdlfyve
       61 prykssfgtr saaasdkssg ndsqlarskg fgseaknkir slvgrkkvkq hsgdgeadts
      121 qcatgkepsl rsfnkaecie vfidvdparh nygrratice alfgiipgsl psrlataeal
      181 eecspvmvqg llpdgeaikt gaikigdvlr gvdgssvnek nidgilrgit kaqkvklqfi
      241 rleentltgc dkftypqnvd aqsslikqls getaivqqvk qqlhqiphaf iyqgfcsdig
      301 qqseparrte qfyrfpdhdh tlsnlrgmfv tlssvlgdvt aakpqtsslt vdghlihvay
      361 fheesgmavl clpaaratpe evhsflleiv kllklehrsl sqafktvenh dcidlfllhf
      421 fremlldtep qrnsfrlmrs lphvhwlhlp meaqghvdtv lselesadms eafhqstrrf
      481 tflgscafyk gfllgnhlpk dylenvflyc rhyqllaltk eesvgqvvvw kevflqdeha
      541 ndrhfvlivg lkhslllsll evggcaslae vnptpdtvyi draqnsllql eflgvltvae
      601 gcllnrssmp gstasstsss wcqsplkdsh ssfflsvpps lsprrarkss dveqchllqc
      661 tssqfdcseh dlqmsrsyss csdsnglrar hcsmstssdc dsrtsgsdiy qnrddklhra
      721 ftssydlssl rrsledgssq gqsharssqp vlcadenlvf hylsieiaeg vfvapvesal
      781 ksnlgvevvd nfyrccleir kvfhcttkea rcaredetdg eskffsdrsl scvrehgvlf
      841 tcpatsssks rakglvsywv vgrlfadkcq prevyvcmhe dvsatalema frlsfglqi
//
LOCUS       XP_077506017             884 aa            linear   INV 25-NOV-2025
DEFINITION  putative helicase MOV-10 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077506017
VERSION     XP_077506017.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649891.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..884
                     /product="putative helicase MOV-10 isoform X1"
                     /calculated_mol_wt=99464
     Region          <325..>501
                     /region_name="McrB"
                     /note="5-methylcytosine-specific restriction endonuclease
                     McrBC, GTP-binding regulatory subunit McrB [Defense
                     mechanisms]; COG1401"
                     /db_xref="CDD:441011"
     Region          437..671
                     /region_name="DEXXQc_Helz-like"
                     /note="DEXXQ/H-box helicase domain of Helz-like helicase;
                     cd18038"
                     /db_xref="CDD:350796"
     Site            order(441,465..470,623,670..671)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350796"
     Region          <523..867
                     /region_name="DNA2"
                     /note="Superfamily I DNA and/or RNA helicase [Replication,
                     recombination and repair]; COG1112"
                     /db_xref="CDD:440729"
     CDS             1..884
                     /gene="LOC144115488"
                     /coded_by="XM_077649891.1:89..2743"
                     /db_xref="GeneID:144115488"
ORIGIN      
        1 mvesingkdd kevvsrkadf scqmcghwsa ledahahfks qehqeehikl clwlnegpll
       61 rnkchvsvhc lttsqvhfsv sirvkllkhv ehkmrvqlss daengvdllh cfllepsnvm
      121 hledygssyd mekhvhlspg svhdvgisck akregvertc lvfkfredtp ekrsfvivrf
      181 vevacyedas gsnipkiqdp ceysqplcrr lpdaeciipa ghaerehqnd psfgnvpaif
      241 ekiclhglkp wknitpyeke slhetekllt tplseknyrq yytallnlek aqlvtdvrsy
      301 dmidvpifks kesklyilev pgakegrpsv ikrsrlffsv mssnaapvte yeaivrkikg
      361 ssvtvklsek fdeiyeegsr fsvrfapnlv plkrmrfavn tcreqqlrsf ifpscappsk
      421 pavsasnltf fnknvqgnpe qskavhdmlr gehrpfpyll fgppgtgkts tivealkqic
      481 tvvpssrilv vspsnsatdf laeqlldhln psqmfrlyss amhpdkiskk lhkysnyteh
      541 eeevqedlps lssfkvivtt lvtsaklmka rlgvnrfthi ivdeagqale peciiplvgl
      601 mspwnpgctg tgghvilagd pmqlgpvvsn qlssshglgv sllerlinmp ayqkngqqss
      661 hmmtkllrnf rshgdllhvp nemfynselq ayadeatsks mllweelptk gvpllfhgvs
      721 grdmqdgtcp sffnleeikv vlgyvtslln wrngiprrvn eedigiispy rkqaakiqne
      781 lekrghkkis vgsteqlqgq ermviiattv rstvgqtskd prhrlgflkn pkrfnvsvtr
      841 akalmvivgn prvlcqdpcw nkmiefcvsk gayrgmkfep snpr
//
LOCUS       XP_077506018             876 aa            linear   INV 25-NOV-2025
DEFINITION  putative helicase MOV-10 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077506018
VERSION     XP_077506018.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649892.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..876
                     /product="putative helicase MOV-10 isoform X2"
                     /calculated_mol_wt=98554
     Region          <317..>493
                     /region_name="McrB"
                     /note="5-methylcytosine-specific restriction endonuclease
                     McrBC, GTP-binding regulatory subunit McrB [Defense
                     mechanisms]; COG1401"
                     /db_xref="CDD:441011"
     Region          429..663
                     /region_name="DEXXQc_Helz-like"
                     /note="DEXXQ/H-box helicase domain of Helz-like helicase;
                     cd18038"
                     /db_xref="CDD:350796"
     Site            order(433,457..462,615,662..663)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350796"
     Region          <515..859
                     /region_name="DNA2"
                     /note="Superfamily I DNA and/or RNA helicase [Replication,
                     recombination and repair]; COG1112"
                     /db_xref="CDD:440729"
     CDS             1..876
                     /gene="LOC144115488"
                     /coded_by="XM_077649892.1:146..2776"
                     /db_xref="GeneID:144115488"
ORIGIN      
        1 mdcsvvsrka dfscqmcghw saledahahf ksqehqeehi klclwlnegp llrnkchvsv
       61 hclttsqvhf svsirvkllk hvehkmrvql ssdaengvdl lhcfllepsn vmhledygss
      121 ydmekhvhls pgsvhdvgis ckakregver tclvfkfred tpekrsfviv rfvevacyed
      181 asgsnipkiq dpceysqplc rrlpdaecii paghaerehq ndpsfgnvpa ifekiclhgl
      241 kpwknitpye keslhetekl lttplsekny rqyytallnl ekaqlvtdvr sydmidvpif
      301 kskesklyil evpgakegrp svikrsrlff svmssnaapv teyeaivrki kgssvtvkls
      361 ekfdeiyeeg srfsvrfapn lvplkrmrfa vntcreqqlr sfifpscapp skpavsasnl
      421 tffnknvqgn peqskavhdm lrgehrpfpy llfgppgtgk tstivealkq ictvvpssri
      481 lvvspsnsat dflaeqlldh lnpsqmfrly ssamhpdkis kklhkysnyt eheeevqedl
      541 pslssfkviv ttlvtsaklm karlgvnrft hiivdeagqa lepeciiplv glmspwnpgc
      601 tgtgghvila gdpmqlgpvv snqlssshgl gvsllerlin mpayqkngqq sshmmtkllr
      661 nfrshgdllh vpnemfynse lqayadeats ksmllweelp tkgvpllfhg vsgrdmqdgt
      721 cpsffnleei kvvlgyvtsl lnwrngiprr vneedigiis pyrkqaakiq nelekrghkk
      781 isvgsteqlq gqermviiat tvrstvgqts kdprhrlgfl knpkrfnvsv trakalmviv
      841 gnprvlcqdp cwnkmiefcv skgayrgmkf epsnpr
//
LOCUS       XP_077506019             788 aa            linear   INV 25-NOV-2025
DEFINITION  putative helicase MOV-10 isoform X3 [Amblyomma americanum].
ACCESSION   XP_077506019
VERSION     XP_077506019.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649893.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..788
                     /product="putative helicase MOV-10 isoform X3"
                     /calculated_mol_wt=88769
     Region          <388..575
                     /region_name="DEAD-like_helicase_N"
                     /note="N-terminal helicase domain of the DEAD-box helicase
                     superfamily; cl28899"
                     /db_xref="CDD:475120"
     Region          <427..771
                     /region_name="DNA2"
                     /note="Superfamily I DNA and/or RNA helicase [Replication,
                     recombination and repair]; COG1112"
                     /db_xref="CDD:440729"
     CDS             1..788
                     /gene="LOC144115488"
                     /coded_by="XM_077649893.1:89..2455"
                     /db_xref="GeneID:144115488"
ORIGIN      
        1 mvesingkdd kevvsrkadf scqmcghwsa ledahahfks qehqeehikl clwlnegpll
       61 rnkchvsvhc lttsqvhfsv sirvkllkhv ehkmrvqlss daengvdllh cfllepsnvm
      121 hledygssyd mekhvhlspg svhdvgisck akregvertc lvfkfredtp ekrsfvivrf
      181 vevacyedas gsnipkiqdp ceysqplcrr lpdaeciipa ghaerehqnd psfgnvpaif
      241 ekiclhglkp wknitpyeke slhetekllt tplseknyrq yytallnlek aqlvtdvrsy
      301 dmidvpifks kesklyilev pgakegrpsv ikrsrlffsv mssnaapvte yeaivrkikg
      361 ssvtvklsek fdeiyeegsr fsictvvpss rilvvspsns atdflaeqll dhlnpsqmfr
      421 lyssamhpdk iskklhkysn yteheeevqe dlpslssfkv ivttlvtsak lmkarlgvnr
      481 fthiivdeag qalepeciip lvglmspwnp gctgtgghvi lagdpmqlgp vvsnqlsssh
      541 glgvsllerl inmpayqkng qqsshmmtkl lrnfrshgdl lhvpnemfyn selqayadea
      601 tsksmllwee lptkgvpllf hgvsgrdmqd gtcpsffnle eikvvlgyvt sllnwrngip
      661 rrvneedigi ispyrkqaak iqnelekrgh kkisvgsteq lqgqermvii attvrstvgq
      721 tskdprhrlg flknpkrfnv svtrakalmv ivgnprvlcq dpcwnkmief cvskgayrgm
      781 kfepsnpr
//
LOCUS       XP_077506020             868 aa            linear   INV 25-NOV-2025
DEFINITION  gamma-aminobutyric acid type B receptor subunit 1 isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077506020
VERSION     XP_077506020.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649894.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..868
                     /product="gamma-aminobutyric acid type B receptor subunit
                     1 isoform X1"
                     /calculated_mol_wt=97521
     Region          42..448
                     /region_name="PBP1_GABAb_receptor"
                     /note="ligand-binding domain of GABAb receptors, which are
                     metabotropic transmembrane receptors for
                     gamma-aminobutyric acid (GABA); cd06366"
                     /db_xref="CDD:380589"
     Site            order(60,141..142,344)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:380589"
     Site            order(96,99..100,102..103,107,128..129,131..133)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:380589"
     Region          468..743
                     /region_name="7tmC_GABA-B-R1"
                     /note="gamma-aminobutyric acid type B receptor subunit 1,
                     member of the class C family of seven-transmembrane G
                     protein-coupled receptors; cd15291"
                     /db_xref="CDD:320418"
     Region          468..493
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320418"
     Site            order(471,474..475,478..479,481..482,516,520,523..524,539,
                     543)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:320418"
     Region          505..526
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320418"
     Site            order(525,544..545,548..549,552,646,651,654..655,658,693,
                     696..697,700,704,714..715,718,721,725)
                     /site_type="other"
                     /note="putative allosteric modulator binding site
                     [chemical binding]"
                     /db_xref="CDD:320418"
     Region          542..566
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320418"
     Region          590..610
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320418"
     Region          647..673
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320418"
     Region          682..705
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320418"
     Region          712..737
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320418"
     Region          720..>801
                     /region_name="MreC"
                     /note="rod shape-determining protein MreC; cl46468"
                     /db_xref="CDD:480808"
     CDS             1..868
                     /gene="GABA-B-R1"
                     /coded_by="XM_077649894.1:356..2962"
                     /db_xref="GeneID:144115489"
ORIGIN      
        1 mgetcaplla vllclaamlr nasslqpnek vetgvqklks lyiaavfpmk ghggwlggqg
       61 clpaalmale dvnqrsdlli gykleidwrd sqcnpglaat vmydllynep qklmllggcs
      121 ivcstvaeaa kmwnlvvisy gssspalsnr krfptffrth psatihnptr iklfqkfsws
      181 riaiiqeaee vfistgedle arckeahiei vtrqsfltdp tdavknlvrq dariivgmfy
      241 vaaarrvfce aykqnvygkq yvwlligwye dgwytvqdkg hnctteqmke aleghfttea
      301 lmlnqgnqet isgmssqqfl eryeralaeq ngglqgykpe ghqeaplayd aiwaialaln
      361 ktintlreys msiedftytn rkiadeiwsa mnatqflgvs gfvafsakgd rmawtlieqm
      421 idgnyvkigy fdtqtdnlti lnqekwadgk ppqdrtiivr vhrkvslslf agmcavafig
      481 vvwavgllif nwifrhsryi qlshpmcnni mligiilclv cvcllgldgq fvsefryahi
      541 cqarswflai gftlsfgamf skiwrvhrlt tkskseskgl sfqrveswkl ygmvgglvlv
      601 davilsawql vdpmqrhlev fpleppalsd edvriepale hcesrnhaiw lgvmysykgl
      661 llifgiflay etrsvkikql ndsrlvgmsi ynvvvlclit apvtlvigsq qdatfafval
      721 aiifcsflsm alifvpkiie lvrrprerad vrslmdtits keeeerhqrl laenedlkkq
      781 iaekeeqiqv lnqklqerqr lahqtalpas gervrialpp tyadgllapk epvaidpvsd
      841 sgfvtgtslv grgsrlsrsd fefsesyl
//
LOCUS       XP_077506021             868 aa            linear   INV 25-NOV-2025
DEFINITION  gamma-aminobutyric acid type B receptor subunit 1 isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077506021
VERSION     XP_077506021.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649895.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..868
                     /product="gamma-aminobutyric acid type B receptor subunit
                     1 isoform X1"
                     /calculated_mol_wt=97521
     Region          42..448
                     /region_name="PBP1_GABAb_receptor"
                     /note="ligand-binding domain of GABAb receptors, which are
                     metabotropic transmembrane receptors for
                     gamma-aminobutyric acid (GABA); cd06366"
                     /db_xref="CDD:380589"
     Site            order(60,141..142,344)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:380589"
     Site            order(96,99..100,102..103,107,128..129,131..133)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:380589"
     Region          468..743
                     /region_name="7tmC_GABA-B-R1"
                     /note="gamma-aminobutyric acid type B receptor subunit 1,
                     member of the class C family of seven-transmembrane G
                     protein-coupled receptors; cd15291"
                     /db_xref="CDD:320418"
     Region          468..493
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320418"
     Site            order(471,474..475,478..479,481..482,516,520,523..524,539,
                     543)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:320418"
     Region          505..526
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320418"
     Site            order(525,544..545,548..549,552,646,651,654..655,658,693,
                     696..697,700,704,714..715,718,721,725)
                     /site_type="other"
                     /note="putative allosteric modulator binding site
                     [chemical binding]"
                     /db_xref="CDD:320418"
     Region          542..566
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320418"
     Region          590..610
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320418"
     Region          647..673
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320418"
     Region          682..705
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320418"
     Region          712..737
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320418"
     Region          720..>801
                     /region_name="MreC"
                     /note="rod shape-determining protein MreC; cl46468"
                     /db_xref="CDD:480808"
     CDS             1..868
                     /gene="GABA-B-R1"
                     /coded_by="XM_077649895.1:356..2962"
                     /db_xref="GeneID:144115489"
ORIGIN      
        1 mgetcaplla vllclaamlr nasslqpnek vetgvqklks lyiaavfpmk ghggwlggqg
       61 clpaalmale dvnqrsdlli gykleidwrd sqcnpglaat vmydllynep qklmllggcs
      121 ivcstvaeaa kmwnlvvisy gssspalsnr krfptffrth psatihnptr iklfqkfsws
      181 riaiiqeaee vfistgedle arckeahiei vtrqsfltdp tdavknlvrq dariivgmfy
      241 vaaarrvfce aykqnvygkq yvwlligwye dgwytvqdkg hnctteqmke aleghfttea
      301 lmlnqgnqet isgmssqqfl eryeralaeq ngglqgykpe ghqeaplayd aiwaialaln
      361 ktintlreys msiedftytn rkiadeiwsa mnatqflgvs gfvafsakgd rmawtlieqm
      421 idgnyvkigy fdtqtdnlti lnqekwadgk ppqdrtiivr vhrkvslslf agmcavafig
      481 vvwavgllif nwifrhsryi qlshpmcnni mligiilclv cvcllgldgq fvsefryahi
      541 cqarswflai gftlsfgamf skiwrvhrlt tkskseskgl sfqrveswkl ygmvgglvlv
      601 davilsawql vdpmqrhlev fpleppalsd edvriepale hcesrnhaiw lgvmysykgl
      661 llifgiflay etrsvkikql ndsrlvgmsi ynvvvlclit apvtlvigsq qdatfafval
      721 aiifcsflsm alifvpkiie lvrrprerad vrslmdtits keeeerhqrl laenedlkkq
      781 iaekeeqiqv lnqklqerqr lahqtalpas gervrialpp tyadgllapk epvaidpvsd
      841 sgfvtgtslv grgsrlsrsd fefsesyl
//
LOCUS       XP_077506022             834 aa            linear   INV 25-NOV-2025
DEFINITION  gamma-aminobutyric acid type B receptor subunit 1 isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077506022
VERSION     XP_077506022.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649896.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..834
                     /product="gamma-aminobutyric acid type B receptor subunit
                     1 isoform X2"
                     /calculated_mol_wt=94003
     Region          42..448
                     /region_name="PBP1_GABAb_receptor"
                     /note="ligand-binding domain of GABAb receptors, which are
                     metabotropic transmembrane receptors for
                     gamma-aminobutyric acid (GABA); cd06366"
                     /db_xref="CDD:380589"
     Site            order(60,141..142,344)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:380589"
     Site            order(96,99..100,102..103,107,128..129,131..133)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:380589"
     Region          468..743
                     /region_name="7tmC_GABA-B-R1"
                     /note="gamma-aminobutyric acid type B receptor subunit 1,
                     member of the class C family of seven-transmembrane G
                     protein-coupled receptors; cd15291"
                     /db_xref="CDD:320418"
     Region          468..493
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320418"
     Site            order(471,474..475,478..479,481..482,516,520,523..524,539,
                     543)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:320418"
     Region          505..526
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320418"
     Site            order(525,544..545,548..549,552,646,651,654..655,658,693,
                     696..697,700,704,714..715,718,721,725)
                     /site_type="other"
                     /note="putative allosteric modulator binding site
                     [chemical binding]"
                     /db_xref="CDD:320418"
     Region          542..566
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320418"
     Region          590..610
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320418"
     Region          647..673
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320418"
     Region          682..705
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320418"
     Region          712..737
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320418"
     Region          720..>824
                     /region_name="MreC"
                     /note="rod shape-determining protein MreC; cl46468"
                     /db_xref="CDD:480808"
     CDS             1..834
                     /gene="GABA-B-R1"
                     /coded_by="XM_077649896.1:356..2860"
                     /db_xref="GeneID:144115489"
ORIGIN      
        1 mgetcaplla vllclaamlr nasslqpnek vetgvqklks lyiaavfpmk ghggwlggqg
       61 clpaalmale dvnqrsdlli gykleidwrd sqcnpglaat vmydllynep qklmllggcs
      121 ivcstvaeaa kmwnlvvisy gssspalsnr krfptffrth psatihnptr iklfqkfsws
      181 riaiiqeaee vfistgedle arckeahiei vtrqsfltdp tdavknlvrq dariivgmfy
      241 vaaarrvfce aykqnvygkq yvwlligwye dgwytvqdkg hnctteqmke aleghfttea
      301 lmlnqgnqet isgmssqqfl eryeralaeq ngglqgykpe ghqeaplayd aiwaialaln
      361 ktintlreys msiedftytn rkiadeiwsa mnatqflgvs gfvafsakgd rmawtlieqm
      421 idgnyvkigy fdtqtdnlti lnqekwadgk ppqdrtiivr vhrkvslslf agmcavafig
      481 vvwavgllif nwifrhsryi qlshpmcnni mligiilclv cvcllgldgq fvsefryahi
      541 cqarswflai gftlsfgamf skiwrvhrlt tkskseskgl sfqrveswkl ygmvgglvlv
      601 davilsawql vdpmqrhlev fpleppalsd edvriepale hcesrnhaiw lgvmysykgl
      661 llifgiflay etrsvkikql ndsrlvgmsi ynvvvlclit apvtlvigsq qdatfafval
      721 aiifcsflsm alifvpkiie lvrrprerad vrslmdtits keeeerhqrl laenedlkkq
      781 iaekeeqiqv lnqklqerqr lahqtalpas gerlgdtgqv gtvlpvhyvr eprm
//
LOCUS       XP_077506023             277 aa            linear   INV 25-NOV-2025
DEFINITION  box C/D snoRNA protein 1 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077506023
VERSION     XP_077506023.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649897.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..277
                     /product="box C/D snoRNA protein 1 isoform X1"
                     /calculated_mol_wt=31361
     Region          6..44
                     /region_name="zf-HIT_BCD1"
                     /note="zinc finger HIT (zf-HIT) found in Box C/D snoRNA
                     protein 1 (BCD1) and similar proteins; cd23023"
                     /db_xref="CDD:467795"
     Site            order(8,11,19,22,27,31,35,41)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:467795"
     CDS             1..277
                     /gene="LOC144115490"
                     /coded_by="XM_077649897.1:121..954"
                     /db_xref="GeneID:144115490"
ORIGIN      
        1 maasgrtclq cgkpskyccp kcsarscslp cvkrhkkige cdgirdktay vpldgftdld
       61 llsdyrflee sarvvdsvcr tkpalgvypq qktlpgflhk fqaecrhrgt mlkllprcft
      121 krqenksqfn frehciywhv awvfpaarik vheiqrvhek eslgrclekh ltmercrpel
      181 rgplqqyita gmqgvsvlla vegkpasqky yynldlsmsl enclkgkviv eyptftviqt
      241 edlcrfnlis pepsgshsat slpaffntnv ladcdne
//
LOCUS       XP_077506024             277 aa            linear   INV 25-NOV-2025
DEFINITION  box C/D snoRNA protein 1 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077506024
VERSION     XP_077506024.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649898.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..277
                     /product="box C/D snoRNA protein 1 isoform X2"
                     /calculated_mol_wt=31410
     Region          6..44
                     /region_name="zf-HIT_BCD1"
                     /note="zinc finger HIT (zf-HIT) found in Box C/D snoRNA
                     protein 1 (BCD1) and similar proteins; cd23023"
                     /db_xref="CDD:467795"
     Site            order(8,11,19,22,27,31,35,41)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:467795"
     CDS             1..277
                     /gene="LOC144115490"
                     /coded_by="XM_077649898.1:122..955"
                     /db_xref="GeneID:144115490"
ORIGIN      
        1 maasgrtclq cgkpskyccp kcsarscslp cvkrhkkige cdgirdktay vpldgftdld
       61 llsdyrflee sarvvdsvcr tkpalgvypq qktlpgflhk fqaecrhrgt mlkllprcft
      121 krqenksqfn frehciywhv awvfpaarik vheiqrvhek eslgrclekh ltmercrpel
      181 rgplqqyita gmqgvsvlla vegkpasqky yynldlsmsl enclkgkviv eyptftviqt
      241 edlcrfnlis pgncqsnlac naywashlqp piarigs
//
LOCUS       XP_077506025             262 aa            linear   INV 25-NOV-2025
DEFINITION  box C/D snoRNA protein 1 isoform X3 [Amblyomma americanum].
ACCESSION   XP_077506025
VERSION     XP_077506025.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649899.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..262
                     /product="box C/D snoRNA protein 1 isoform X3"
                     /calculated_mol_wt=29884
     Region          6..44
                     /region_name="zf-HIT_BCD1"
                     /note="zinc finger HIT (zf-HIT) found in Box C/D snoRNA
                     protein 1 (BCD1) and similar proteins; cd23023"
                     /db_xref="CDD:467795"
     Site            order(8,11,19,22,27,31,35,41)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:467795"
     CDS             1..262
                     /gene="LOC144115490"
                     /coded_by="XM_077649899.1:121..909"
                     /db_xref="GeneID:144115490"
ORIGIN      
        1 maasgrtclq cgkpskyccp kcsarscslp cvkrhkkige cdgirdktay vpldgftdld
       61 llsdyrflee sarvvdsvcr tkpalgvypq qktlpgflhk fqaecrhrgt mlkllprcft
      121 krqenksqfn frehciywhv awvfpaarik vheiqrvhek eslgrclekh ltmercrpel
      181 rgplqqyita gmqgvsvlla vegkpasqky yynldlsmsl enclkgkviv eyptftviqt
      241 edlcrfnlis pvsmqwgqal ln
//
LOCUS       XP_077506026             262 aa            linear   INV 25-NOV-2025
DEFINITION  box C/D snoRNA protein 1 isoform X4 [Amblyomma americanum].
ACCESSION   XP_077506026
VERSION     XP_077506026.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649900.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..262
                     /product="box C/D snoRNA protein 1 isoform X4"
                     /calculated_mol_wt=29644
     Region          6..44
                     /region_name="zf-HIT_BCD1"
                     /note="zinc finger HIT (zf-HIT) found in Box C/D snoRNA
                     protein 1 (BCD1) and similar proteins; cd23023"
                     /db_xref="CDD:467795"
     Site            order(8,11,19,22,27,31,35,41)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:467795"
     CDS             1..262
                     /gene="LOC144115490"
                     /coded_by="XM_077649900.1:121..909"
                     /db_xref="GeneID:144115490"
ORIGIN      
        1 maasgrtclq cgkpskyccp kcsarscslp cvkrhkkige cdgirdktay vpldgftdld
       61 llsdyrflee sarvvdsvcr tkpalgvypq qktlpgflhk fqaecrhrgt mlkllprcft
      121 krqenksqfn frehciywhv awvfpaarik vheiqrvhek eslgrclekh ltmercrpel
      181 rgplqqyita gmqgvsvlla vegkpasqky yynldlsmsl enclkgkviv eyptftviqt
      241 edlcrfnlis paagdcdvai gd
//
LOCUS       XP_077506027             255 aa            linear   INV 25-NOV-2025
DEFINITION  multivesicular body subunit 12-like Mvb12 [Amblyomma americanum].
ACCESSION   XP_077506027
VERSION     XP_077506027.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649901.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..255
                     /product="multivesicular body subunit 12-like Mvb12"
                     /calculated_mol_wt=28875
     Region          13..249
                     /region_name="DUF2464"
                     /note="Multivesicular body subunit 12; pfam10240"
                     /db_xref="CDD:463017"
     CDS             1..255
                     /gene="Mvb12"
                     /coded_by="XM_077649901.1:108..875"
                     /db_xref="GeneID:144115491"
ORIGIN      
        1 mmqdllkvlp ddlpitdvci iedpqncppg yhvvdktfdq dtdadlwkdr ffgrritryi
       61 cyskahhtec iqsllvvgdk evppeghivm nstkdtdqra mkkkllcykm vardsageav
      121 sdiivlsksr kppdgfvlag evnslhigyk ltsvapvngr knsyhqpplp plpyplpshv
      181 ddhlqkkspg grvcqrgksm qksaiddvpf evnpkyramf rlseftmpni riktaneife
      241 eynynfqven qaqqt
//
LOCUS       XP_077506028             255 aa            linear   INV 25-NOV-2025
DEFINITION  multivesicular body subunit 12-like Mvb12 [Amblyomma americanum].
ACCESSION   XP_077506028
VERSION     XP_077506028.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649902.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..255
                     /product="multivesicular body subunit 12-like Mvb12"
                     /calculated_mol_wt=28875
     Region          13..249
                     /region_name="DUF2464"
                     /note="Multivesicular body subunit 12; pfam10240"
                     /db_xref="CDD:463017"
     CDS             1..255
                     /gene="Mvb12"
                     /coded_by="XM_077649902.1:93..860"
                     /db_xref="GeneID:144115491"
ORIGIN      
        1 mmqdllkvlp ddlpitdvci iedpqncppg yhvvdktfdq dtdadlwkdr ffgrritryi
       61 cyskahhtec iqsllvvgdk evppeghivm nstkdtdqra mkkkllcykm vardsageav
      121 sdiivlsksr kppdgfvlag evnslhigyk ltsvapvngr knsyhqpplp plpyplpshv
      181 ddhlqkkspg grvcqrgksm qksaiddvpf evnpkyramf rlseftmpni riktaneife
      241 eynynfqven qaqqt
//
LOCUS       XP_077506029             255 aa            linear   INV 25-NOV-2025
DEFINITION  multivesicular body subunit 12-like Mvb12 [Amblyomma americanum].
ACCESSION   XP_077506029
VERSION     XP_077506029.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649903.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..255
                     /product="multivesicular body subunit 12-like Mvb12"
                     /calculated_mol_wt=28875
     Region          13..249
                     /region_name="DUF2464"
                     /note="Multivesicular body subunit 12; pfam10240"
                     /db_xref="CDD:463017"
     CDS             1..255
                     /gene="Mvb12"
                     /coded_by="XM_077649903.1:113..880"
                     /db_xref="GeneID:144115491"
ORIGIN      
        1 mmqdllkvlp ddlpitdvci iedpqncppg yhvvdktfdq dtdadlwkdr ffgrritryi
       61 cyskahhtec iqsllvvgdk evppeghivm nstkdtdqra mkkkllcykm vardsageav
      121 sdiivlsksr kppdgfvlag evnslhigyk ltsvapvngr knsyhqpplp plpyplpshv
      181 ddhlqkkspg grvcqrgksm qksaiddvpf evnpkyramf rlseftmpni riktaneife
      241 eynynfqven qaqqt
//
LOCUS       XP_077506030             255 aa            linear   INV 25-NOV-2025
DEFINITION  multivesicular body subunit 12-like Mvb12 [Amblyomma americanum].
ACCESSION   XP_077506030
VERSION     XP_077506030.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649904.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..255
                     /product="multivesicular body subunit 12-like Mvb12"
                     /calculated_mol_wt=28875
     Region          13..249
                     /region_name="DUF2464"
                     /note="Multivesicular body subunit 12; pfam10240"
                     /db_xref="CDD:463017"
     CDS             1..255
                     /gene="Mvb12"
                     /coded_by="XM_077649904.1:46..813"
                     /db_xref="GeneID:144115491"
ORIGIN      
        1 mmqdllkvlp ddlpitdvci iedpqncppg yhvvdktfdq dtdadlwkdr ffgrritryi
       61 cyskahhtec iqsllvvgdk evppeghivm nstkdtdqra mkkkllcykm vardsageav
      121 sdiivlsksr kppdgfvlag evnslhigyk ltsvapvngr knsyhqpplp plpyplpshv
      181 ddhlqkkspg grvcqrgksm qksaiddvpf evnpkyramf rlseftmpni riktaneife
      241 eynynfqven qaqqt
//
LOCUS       XP_077506031             255 aa            linear   INV 25-NOV-2025
DEFINITION  multivesicular body subunit 12-like Mvb12 [Amblyomma americanum].
ACCESSION   XP_077506031
VERSION     XP_077506031.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649905.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..255
                     /product="multivesicular body subunit 12-like Mvb12"
                     /calculated_mol_wt=28875
     Region          13..249
                     /region_name="DUF2464"
                     /note="Multivesicular body subunit 12; pfam10240"
                     /db_xref="CDD:463017"
     CDS             1..255
                     /gene="Mvb12"
                     /coded_by="XM_077649905.1:103..870"
                     /db_xref="GeneID:144115491"
ORIGIN      
        1 mmqdllkvlp ddlpitdvci iedpqncppg yhvvdktfdq dtdadlwkdr ffgrritryi
       61 cyskahhtec iqsllvvgdk evppeghivm nstkdtdqra mkkkllcykm vardsageav
      121 sdiivlsksr kppdgfvlag evnslhigyk ltsvapvngr knsyhqpplp plpyplpshv
      181 ddhlqkkspg grvcqrgksm qksaiddvpf evnpkyramf rlseftmpni riktaneife
      241 eynynfqven qaqqt
//
LOCUS       XP_077506032             214 aa            linear   INV 25-NOV-2025
DEFINITION  vesicle-trafficking protein SEC22 [Amblyomma americanum].
ACCESSION   XP_077506032
VERSION     XP_077506032.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649906.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..214
                     /product="vesicle-trafficking protein SEC22"
                     /calculated_mol_wt=24602
     Region          3..127
                     /region_name="Longin"
                     /note="longin domain; cd14824"
                     /db_xref="CDD:341428"
     Site            order(3,34,37,57,60,62)
                     /site_type="lipid-binding"
                     /note="lipid binding site [chemical binding]"
                     /db_xref="CDD:341428"
     Site            order(15,18,20,35,39,118,122,125)
                     /site_type="other"
                     /note="Sec22 interface [polypeptide binding]"
                     /db_xref="CDD:341428"
     Site            order(56,59,79,83)
                     /site_type="other"
                     /note="VARP interface [polypeptide binding]"
                     /db_xref="CDD:341428"
     Region          133..196
                     /region_name="R-SNARE_SEC22"
                     /note="SNARE motif of SEC22; cd15866"
                     /db_xref="CDD:277219"
     Site            order(135..137,139..140,142..147,149..151,153,156..157,
                     159..161,163..168,170..175,177..182,184..189,191..193)
                     /site_type="other"
                     /note="heterotetramer interface [polypeptide binding]"
                     /db_xref="CDD:277219"
     Site            160
                     /site_type="other"
                     /note="zero layer"
                     /db_xref="CDD:277219"
     CDS             1..214
                     /gene="Sec22"
                     /coded_by="XM_077649906.1:135..779"
                     /db_xref="GeneID:144115494"
ORIGIN      
        1 mvlmtmiarv tdglplsasv qddqqqhgvg yteyqnqakm lfkklnnqsf srstietgpy
       61 nfhymiengv cylvlteksf skrlafsfle dlqnefnsrh gskvhsvnrp ysfiefdtyi
      121 qkakksymds rarrnlssln selqdvqrim vqniddvlqr gsviseldsk asnlsllsqk
      181 ykkdarylnl rstyakiaav svivfvafly fyif
//
LOCUS       XP_077506033             141 aa            linear   INV 25-NOV-2025
DEFINITION  SNAP associated protein [Amblyomma americanum].
ACCESSION   XP_077506033
VERSION     XP_077506033.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649907.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="SNAP associated protein"
                     /calculated_mol_wt=15752
     Region          30..117
                     /region_name="Snapin_Pallidin"
                     /note="Snapin/Pallidin; pfam14712"
                     /db_xref="CDD:464275"
     CDS             1..141
                     /gene="Snapin"
                     /coded_by="XM_077649907.1:40..465"
                     /db_xref="GeneID:144115495"
ORIGIN      
        1 maacsimdds enatsiddrs eayseasard citegiigll lptveqider vkstrisqle
       61 lrqhidtlae dlrvlsenqq ipydldsyvk klvnakrrvv lvnsilqntq erlnrinqsi
      121 trenerrtal lepqgsvpge g
//
LOCUS       XP_077506034             503 aa            linear   INV 25-NOV-2025
DEFINITION  26S proteasome non-ATPase regulatory subunit 5 [Amblyomma
            americanum].
ACCESSION   XP_077506034
VERSION     XP_077506034.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649908.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..503
                     /product="26S proteasome non-ATPase regulatory subunit 5"
                     /calculated_mol_wt=55675
     Region          11..500
                     /region_name="Proteasom_PSMB"
                     /note="Proteasome non-ATPase 26S subunit; pfam10508"
                     /db_xref="CDD:463124"
     CDS             1..503
                     /gene="LOC144115496"
                     /coded_by="XM_077649908.1:54..1565"
                     /db_xref="GeneID:144115496"
ORIGIN      
        1 maadlkvvla allreiserg scdnlaevkt ilsatraeni ynvskdldls plfnclnsfs
       61 gdtlnelsel lgklldafpp tallaahkqh fvaalgsshd tlkavilrql ircskdnatl
      121 cqlardhril esilwllgge dlwsskqsat llkqmireke vlsamlhppv ahvldsvmdh
      181 gdvcrfrvfe vfvdvakasp dnlerlsesg hmerllkemd kndvlvqlna levltelvsc
      241 shgldylert giisklrlka lnissdplgn flgpgvlkff gsmgvtspqr llldypqvlg
      301 fifesvqsde ptyqvvaaet vgiisstiqg krvlqshedq msrylktlrq riesgkvdfk
      361 vrylgalsnv lhiqdrsced lerltehwfg llgqepmkml lslcrqpfpe lrcaalavva
      421 evartawgqa mlsvqpgfle ylldrstead kqgkeakfai vaclaqappr gfaaddwqri
      481 rtvyhegpfy aaadaavtfe gls
//
LOCUS       XP_077506035             823 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077506035
VERSION     XP_077506035.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649909.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..823
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=91503
     Region          101..230
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          328..734
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..823
                     /gene="LOC144115500"
                     /coded_by="XM_077649909.1:161..2632"
                     /db_xref="GeneID:144115500"
ORIGIN      
        1 malgsarcsr glllllfaaa sslvsaqlas drstgdvadp vaasgttamt trttttttpq
       61 pvdllketld siseramria mkelypmvse liydprlspg cigslmkigp alrnfeiwav
      121 emvdamgkpq agmalgriam ygqydeclgi rhheglfqgr ycmvhlshda spmpptvvka
      181 aekfmkhhkl eyignvseva tneittivpl lkygacvpsv cqqedvqvii dhlvgdlkvn
      241 lkaawckide pvrldqrqtl ivcifvlwta filfgtaydi yrtvvaaeda klpkynafmg
      301 lvheavqsvs lrrafkklme mpnwgdysnk lgfihgvrvf satwvvlghs hmlrdlhans
      361 nmirllrrvq edflftvqln sfmavetfla itgflsgylv akahrvdvsp lllyivalvr
      421 ryirlmvpma allgfvylip aivdgpplhd ywpllmkpcd knwwkiftms qnymddykdl
      481 cmphywyism eyqlaiysti ilvaifprwp kvalwlmgas vaatclttgi qvyinnytpf
      541 nvvfttdvqr iidssmniyi kaythappmf igmifgclav rrnqlsrliq gaawalaatv
      601 slaallgvrt wfdgrqperl esafyaalhr aswslgaswv myacatghgg fvnkilawpv
      661 lyplgrlsfs vylvhvilmg cnavlsreni sqqpflqaqa ylsmaimsyv lgsivylfie
      721 cpvaglenal fskiipkkaf msdntkfkde nqelkaiqvs sadaagktwd valpkhecln
      781 gfpdvtknav ngshlngyvn sacesefsek dtapggsvvt vkf
//
LOCUS       XP_077506036             302 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077506036
VERSION     XP_077506036.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649910.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..302
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=32752
     Region          <42..210
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..302
                     /gene="LOC144115501"
                     /coded_by="XM_077649910.1:104..1012"
                     /db_xref="GeneID:144115501"
ORIGIN      
        1 mgtiaaaacl atgiqvyvqe slpfnlvvtt dikklidmhl diylmahtha pplfvgiifg
       61 clatrrhrms slaqgaawlv aatvslaalm gvrtwnvgrq perlesaiya glhraswglg
      121 vawvmyacat krggfvnkil awpifyplgr lsfsvylvhl ilmacnsvls rervtqhpfl
      181 haqmyvstai tayalasivy lliecpvaal dnlvfhkmmp ketilkviqg kgtaqevkai
      241 qalaegkkkk pvespakgkn iselpdftki nldghhchvn gaceidsned tsangavisa
      301 kf
//
LOCUS       XP_077506037             890 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077506037
VERSION     XP_077506037.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649911.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..890
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=99120
     Region          216..343
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          441..848
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..890
                     /gene="LOC144115502"
                     /coded_by="XM_077649911.1:6..2678"
                     /db_xref="GeneID:144115502"
ORIGIN      
        1 mythaqktga qslppppctl pafpalvrrp sggrqivvvp pdfleelerg egpqeegrah
       61 grtaaedgkq rgekrrreaq qnrerkkakg mkegtpcspf rrkefqffae apsennnarp
      121 ftasracvat swalpamwtr ttlaaclclv aaatattppq vsravadeap lagattvsdd
      181 apdvegrlrs lmdrlihesl prmtalgsgs nvtgdcsaal frlllgmrkf epwalrllda
      241 nsrppgglis gtfndlgtyd qclstaarda dggvhfsgqy ctlylqprrl pffekivrrl
      301 hdevydgakl dffrfmyndm ahglrlgvcf psacsarefq fligslvnqh gfdatvkgcv
      361 ikektplstt ekalmyfmas cisfvivgsl tdiylrkygk eaghdlnnig tffkvvlsfs
      421 avanteklln vraergsdaw rlrflhglrf lsaswvilgh syltveptav gellrivrfg
      481 knfiwclvgn aypsvqtflf msgfllsfni arhlkkrkgs lwlpvtlllv rryirltapl
      541 mfvigvwllv plvvdgplak ehhpsifatc trnwwralih innwsdlldm clqhswyvsv
      601 dwqiymfiwi ipvvmlsrpr igllfagala igtsaavtvn ayvysyqplp lygqpeidkt
      661 ldmmkfiyyr pyvhvtsyav gimlgyavsa narlqlpcwa raslwalstt lallvvfgpf
      721 kwlrgdswrg gdavayagfs klawalslsw vvfacasgrg gfanrllswk pliplsrlsy
      781 gaylihsply liragimrer mslqhfnlvk dffgcltmsf lmayllylvc eapvaslekl
      841 mlapatrriq aaaekdspsd alqgergree vcvtvasaqq hviseskcrl
//
LOCUS       XP_077506038             265 aa            linear   INV 25-NOV-2025
DEFINITION  LIM/homeobox protein Lhx9-like [Amblyomma americanum].
ACCESSION   XP_077506038
VERSION     XP_077506038.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649912.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..265
                     /product="LIM/homeobox protein Lhx9-like"
                     /calculated_mol_wt=29595
     Region          43..96
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(43,46,64,67,70,73,92,95)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:259829"
     Region          102..158
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(103,106,126,129,132,135,154,157)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188763"
     Region          169..225
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..265
                     /gene="LOC144115503"
                     /coded_by="XM_077649912.1:119..916"
                     /db_xref="GeneID:144115503"
ORIGIN      
        1 mmvsrprspt erlvspslea adlkqglcqr raleqgaggl lacsgcrgli rerfylqald
       61 qpwhtgclrc clcgtgldsq ascfaregri ycrddylraf vrccacgaal sprdlvmrvr
      121 gglafhvgcf rcasclcpll kgdvfgmrdn svfcklhfqd taliaetnkr mrtsfkhsql
      181 ramkayftvn hnpdskdlkq lslrtglskr vlqvwfqnar akwrrnllrq ashgpevaqd
      241 ssplqdlgat itleeqtihs yanlf
//
LOCUS       XP_077506039             352 aa            linear   INV 25-NOV-2025
DEFINITION  LIM/homeobox protein Lhx9-like [Amblyomma americanum].
ACCESSION   XP_077506039
VERSION     XP_077506039.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649913.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..352
                     /product="LIM/homeobox protein Lhx9-like"
                     /calculated_mol_wt=38629
     Region          23..76
                     /region_name="LIM1_Lhx2_Lhx9"
                     /note="The first LIM domain of Lhx2 and Lhx9 family;
                     cd09369"
                     /db_xref="CDD:188755"
     Site            order(23,26,44,47,50,53,72,75)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188755"
     Region          81..139
                     /region_name="LIM2_Lhx2_Lhx9"
                     /note="The second LIM domain of Lhx2 and Lhx9 family;
                     cd09377"
                     /db_xref="CDD:188763"
     Site            order(85,88,107,110,113,116,135,138)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188763"
     Region          238..294
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..352
                     /gene="LOC144115505"
                     /coded_by="XM_077649913.1:449..1507"
                     /db_xref="GeneID:144115505"
ORIGIN      
        1 mpvispgpde aaavqalapp klcagcgapi adrfyllave rqwhtrclrc chckqqldse
       61 ltcfardgni yckddyyrlf avkrcarcqq gifaselvmr ardlvyhlhc ftcawcnaal
      121 aqgdhfglrd nlvycrthfe llaegcqpgl pppppllqdd dagggggaag acspplaqgp
      181 ayvppqgpyg vrkgrprkrk pgdlpegpph lglqdlagld sngspmlqqq qqqqqrtkrm
      241 rtsfkhhqlr tmksyfainq npdakdlkql aqktglskrv lqvwfqnara kwrrnnlrqq
      301 eqpttslpas spggtssfse pspgapldys naqttlvvta sqeslgsfqe lf
//
LOCUS       XP_077506040             414 aa            linear   INV 25-NOV-2025
DEFINITION  cholesterol 7-desaturase nvd-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506040
VERSION     XP_077506040.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649914.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..414
                     /product="cholesterol 7-desaturase nvd-like isoform X1"
                     /calculated_mol_wt=46510
     Region          60..>237
                     /region_name="PobA"
                     /note="Chlorophyllide a oxygenase/letal leaf spot protein
                     [Coenzyme transport and metabolism]; COG5749"
                     /db_xref="CDD:444459"
     Region          182..397
                     /region_name="KshA_C"
                     /note="3-Ketosteroid 9alpha-hydroxylase C-terminal domain;
                     pfam19298"
                     /db_xref="CDD:466031"
     CDS             1..414
                     /gene="LOC144115506"
                     /coded_by="XM_077649914.1:67..1311"
                     /db_xref="GeneID:144115506"
ORIGIN      
        1 maeelwlqqc arlqafafsg ngipfgpqpv lvaiavatvv llcafifskr crgnesekkq
       61 kvrtvspgdv ppvfpngwip vlessdlkkg evkavsvigq dlvafrtedg tarvfhaycp
      121 hlgahlgvmg rvvddciecp fhgwrfqadt gacthvpyae kvptfgkakt wtckellgyv
      181 fiwyhadgee psydiptdal eegmeretsc asstgvrghi qdiaengadl ahfnqihwan
      241 glvngieyrk nqgeswmgri fkhhwspvws yenhvgtvtl nssisvlgwk pdflrhhtva
      301 rtigpaltii hirgsatnym vvqavmpegp fnikvvhrvh fqprmswflk qlyviglrnm
      361 vdrdgivwns kvlhkkpala keeqpiaafr kwysqfysas sptwqeireq slew
//
LOCUS       XP_077506041             377 aa            linear   INV 25-NOV-2025
DEFINITION  cholesterol 7-desaturase nvd-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506041
VERSION     XP_077506041.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649915.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..377
                     /product="cholesterol 7-desaturase nvd-like isoform X2"
                     /calculated_mol_wt=41816
     Region          60..>237
                     /region_name="PobA"
                     /note="Chlorophyllide a oxygenase/letal leaf spot protein
                     [Coenzyme transport and metabolism]; COG5749"
                     /db_xref="CDD:444459"
     Region          182..>309
                     /region_name="KshA_C"
                     /note="3-Ketosteroid 9alpha-hydroxylase C-terminal domain;
                     pfam19298"
                     /db_xref="CDD:466031"
     CDS             1..377
                     /gene="LOC144115506"
                     /coded_by="XM_077649915.1:67..1200"
                     /db_xref="GeneID:144115506"
ORIGIN      
        1 maeelwlqqc arlqafafsg ngipfgpqpv lvaiavatvv llcafifskr crgnesekkq
       61 kvrtvspgdv ppvfpngwip vlessdlkkg evkavsvigq dlvafrtedg tarvfhaycp
      121 hlgahlgvmg rvvddciecp fhgwrfqadt gacthvpyae kvptfgkakt wtckellgyv
      181 fiwyhadgee psydiptdal eegmeretsc asstgvrghi qdiaengadl ahfnqihwan
      241 glvngieyrk nqgeswmgri fkhhwspvws yenhvgtvtl nssisvlgwk pdflrhhtva
      301 rtigpaltii hirgsatnym vvqavmpegp fnikvvhrvh fqprmswflk qlyviglrnm
      361 vvaprrcrsl aaadsva
//
LOCUS       XP_077506042             294 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115507 [Amblyomma americanum].
ACCESSION   XP_077506042
VERSION     XP_077506042.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649916.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 29% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..294
                     /product="uncharacterized protein LOC144115507"
                     /calculated_mol_wt=33145
     Region          63..145
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     CDS             1..294
                     /gene="LOC144115507"
                     /coded_by="XM_077649916.1:1..885"
                     /db_xref="GeneID:144115507"
ORIGIN      
        1 mpeaahhqav qvgslglcsg slrqrdpalf sgtdnrdaed wltsyerlst ynnwddttkl
       61 nnvffyltgv anlwfrnhea dlstwtafka nltevfggpa vrklraehrf rersrqsget
      121 ftsyiedvvn lcsrvnpqms eedkikhilk iiedddfqml lsrdlrtvae vvslcqsyde
      181 lrkqrlitrq haaavvesla glsaapdrsq llvhmkdfvr eevagqllll pspsvqspfl
      241 apdlptaise qlqplqcppr pfdcvgiyiy gplpytaagn rwvivaidhl trmq
//
LOCUS       XP_077506043             243 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115508 [Amblyomma americanum].
ACCESSION   XP_077506043
VERSION     XP_077506043.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649917.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..243
                     /product="uncharacterized protein LOC144115508"
                     /calculated_mol_wt=27115
     Region          9..91
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..243
                     /gene="LOC144115508"
                     /coded_by="XM_077649917.1:857..1588"
                     /db_xref="GeneID:144115508"
ORIGIN      
        1 messcspark lwseretaal idcwqdrlnd mrrqkrnvav yaeiaealri lgfhrtsagv
       61 rhkirnltqm yrdfnkngtt tgsgaiqwph yarihsflgs lpmndpslvq etrcsggatv
      121 eeiilgmvvd adtgtaedgs palenwqepa lnaddsragg faaesatied ggaegqagir
      181 eqqnnrkrlr tassdfrqal lteqrmlres leachsreme mrqrhltlqe kmvnaitkff
      241 ess
//
LOCUS       XP_077506044             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115509 [Amblyomma americanum].
ACCESSION   XP_077506044
VERSION     XP_077506044.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649918.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144115509"
                     /calculated_mol_wt=24573
     CDS             1..217
                     /gene="LOC144115509"
                     /coded_by="XM_077649918.1:631..1284"
                     /db_xref="GeneID:144115509"
ORIGIN      
        1 mslqrkrgia lmmveaadle veieaakcqt dsvkrrllfa nglciaatva cdgtvqrerw
       61 mynrnerwfe dtlphlgerf fkqscrvtpt fryiveacrp llqrvdknmr laisvekrva
      121 vamyklcssa edrsvanlfg lgrstvntiy refcdavvcl ledkwinmps pdamgdhire
      181 fgavcdfpqa vgaldgchfp vsppkesapd yynykgw
//
LOCUS       XP_077506045             320 aa            linear   INV 25-NOV-2025
DEFINITION  solute carrier family 25 member 35-like [Amblyomma americanum].
ACCESSION   XP_077506045
VERSION     XP_077506045.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649919.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..320
                     /product="solute carrier family 25 member 35-like"
                     /calculated_mol_wt=35299
     Region          1..96
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          105..204
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          210..302
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     CDS             1..320
                     /gene="LOC144115510"
                     /coded_by="XM_077649919.1:296..1258"
                     /db_xref="GeneID:144115510"
ORIGIN      
        1 maevliaava scsaslithp ievakiriql qgelqspvkd klarkrpvyr nvpqalytva
       61 rydgifaiyr gllpglgyql langlrlgmf qtienfgftl denlepsirl saisggvaga
      121 vgafagsplf llkthmqvhc atasiavgyq hgyrtaraaf lhiyrtqglw nglwratssn
      181 ilristgsal qlstfigfkt llntategre ryffintvla afisglvsap fiavvdvlra
      241 rmyaqpsnar geglyyrsvw dcvrkikrte glrglskglg gaflytltts sitliswdel
      301 knarrerrhs rdyyidfgyq
//
LOCUS       XP_077506046              73 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin-like protein 5 [Amblyomma americanum].
ACCESSION   XP_077506046
VERSION     XP_077506046.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649920.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..73
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..73
                     /product="ubiquitin-like protein 5"
                     /calculated_mol_wt=8533
     Region          1..71
                     /region_name="Ubl_UBL5"
                     /note="ubiquitin-like (Ubl) domain found in ubiquitin-like
                     protein 5 (UBL5) and similar proteins; cd01791"
                     /db_xref="CDD:340489"
     Site            order(1,15,17..19,22,26,29..30,33..34)
                     /site_type="other"
                     /note="HIND interaction site [polypeptide binding]"
                     /db_xref="CDD:340489"
     CDS             1..73
                     /gene="ubl"
                     /coded_by="XM_077649920.1:140..361"
                     /db_xref="GeneID:144115511"
ORIGIN      
        1 mieitcndrl gkkvrvkcnp ddtiadlkkl iaaqtgtrwe kivlkkwytv fkdniklddy
       61 eihdgmnlel yyq
//
LOCUS       XP_077506047             324 aa            linear   INV 25-NOV-2025
DEFINITION  lim3 homeobox protein isoform X1 [Amblyomma americanum].
ACCESSION   XP_077506047
VERSION     XP_077506047.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649921.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..324
                     /product="lim3 homeobox protein isoform X1"
                     /calculated_mol_wt=35853
     Region          30..85
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(33,36,54,57,60,63,80,83)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188850"
     Region          92..147
                     /region_name="LIM2_Lhx3_Lhx4"
                     /note="The second LIM domain of Lhx3-Lhx4 family; cd09376"
                     /db_xref="CDD:188762"
     Site            order(92,95,114,117,120,123,143,146)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188762"
     Site            order(104,129)
                     /site_type="other"
                     /note="Isl binding site"
                     /db_xref="CDD:188762"
     Region          158..214
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..324
                     /gene="Lim3"
                     /coded_by="XM_077649921.1:376..1350"
                     /db_xref="GeneID:144115512"
ORIGIN      
        1 mglpeqtmtv sislstmfnp fchpvlassi pkcagcekpi ldrfilkvle rswharclkc
       61 adcqaqlank cfarnghvyc kddffkrygt kcagcelgip ptqvvrraqd nvyhlhcfac
      121 ilckrqlntg defylmednk lvckadyeaa karegsskrp rttitakqle tlksaynnsp
      181 kparhvreql sqdtgldmrv vqvwfqnrra kekrlkkdag ktrwgdffrs ssggtikrdv
      241 kdhpedsepf gltgsdpegs pgqqsghsga ssyfpaspgg qlgphfglpg dqfpgrgddt
      301 ygefppspas wledmdstps sanf
//
LOCUS       XP_077506048             324 aa            linear   INV 25-NOV-2025
DEFINITION  lim3 homeobox protein isoform X2 [Amblyomma americanum].
ACCESSION   XP_077506048
VERSION     XP_077506048.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649922.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..324
                     /product="lim3 homeobox protein isoform X2"
                     /calculated_mol_wt=35874
     Region          30..85
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(33,36,54,57,60,63,80,83)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188850"
     Region          92..147
                     /region_name="LIM2_Lhx3_Lhx4"
                     /note="The second LIM domain of Lhx3-Lhx4 family; cd09376"
                     /db_xref="CDD:188762"
     Site            order(92,95,114,117,120,123,143,146)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188762"
     Site            order(104,129)
                     /site_type="other"
                     /note="Isl binding site"
                     /db_xref="CDD:188762"
     Region          158..214
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..324
                     /gene="Lim3"
                     /coded_by="XM_077649922.1:129..1103"
                     /db_xref="GeneID:144115512"
ORIGIN      
        1 msatsmvtad hnrdmllall srnkaleasi pkcagcekpi ldrfilkvle rswharclkc
       61 adcqaqlank cfarnghvyc kddffkrygt kcagcelgip ptqvvrraqd nvyhlhcfac
      121 ilckrqlntg defylmednk lvckadyeaa karegsskrp rttitakqle tlksaynnsp
      181 kparhvreql sqdtgldmrv vqvwfqnrra kekrlkkdag ktrwgdffrs ssggtikrdv
      241 kdhpedsepf gltgsdpegs pgqqsghsga ssyfpaspgg qlgphfglpg dqfpgrgddt
      301 ygefppspas wledmdstps sanf
//
LOCUS       XP_077506049             449 aa            linear   INV 25-NOV-2025
DEFINITION  tubulin alpha-1C chain-like [Amblyomma americanum].
ACCESSION   XP_077506049
VERSION     XP_077506049.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649923.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..449
                     /product="tubulin alpha-1C chain-like"
                     /calculated_mol_wt=49840
     Region          1..448
                     /region_name="PTZ00335"
                     /note="tubulin alpha chain; Provisional"
                     /db_xref="CDD:185562"
     CDS             1..449
                     /gene="LOC144115513"
                     /coded_by="XM_077649923.1:136..1485"
                     /db_xref="GeneID:144115513"
ORIGIN      
        1 mrecisvhig qagvqignac welyclehgi qqdgqmpsdk vsggiddsfn tffaetgagr
       61 hvpravfvdl eptvvdevrs gayrqlyhpe qlisgkedaa nnyarghyti gkeivdlvld
      121 rirkladqct glqgflvfhs fgggtgsgft sllmerlsvd ygkksklefs iypapqvsta
      181 vvepynsilt thttlehsdc afmvdneaiy dicrrnldid rptytnlnrl isqivssita
      241 slrfdgalnv dltefqtnlv pyprihfplv tyapvisaek ayheqitvae itnacfepan
      301 qmvkcdprhg kymaccmlyr gdvvpkdvna aiaaiktkrt iqfvdwcptg fkvginyqpp
      361 tvvpggdlak vpravcmlsn ttaiaeawar ldhkfdlmya krafvhwyvg egmeegefse
      421 aredmaalek dyeevgmdsn egadeseey
//
LOCUS       XP_077506050             931 aa            linear   INV 25-NOV-2025
DEFINITION  WD repeat-containing protein 47-like [Amblyomma americanum].
ACCESSION   XP_077506050
VERSION     XP_077506050.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649924.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..931
                     /product="WD repeat-containing protein 47-like"
                     /calculated_mol_wt=101376
     Region          45..94
                     /region_name="CTLH"
                     /note="C-terminal to LisH motif; smart00668"
                     /db_xref="CDD:128914"
     Region          507..929
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          618..666
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          671..714
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          720..761
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          766..801
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          815..850
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          856..894
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          902..928
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..931
                     /gene="LOC144115515"
                     /coded_by="XM_077649924.1:222..3017"
                     /db_xref="GeneID:144115515"
ORIGIN      
        1 maahlcleea evvrlvlefl anrelsisql aleresgvin dalsddllfl rqlildgqwd
       61 nvldfvqple dmeafdskrf kfsvlkhkfl ellcirseag lvqsaelgvd dmvaclgale
      121 qcspsrehyn qlcllltfpr lcdhldykhw npsnarincf revlplvekl lpapvkspvs
      181 vqrtssgdrl vqllikgily escvnlcqkk atlppcstrd eefhfaellg nadadlslfs
      241 wlqclppevf sypfeqkvln vdirqlvkpf leaswteqil atpikprvfp hsavprtpms
      301 rslimasmeg glfsagshpa lsggakmals madltssmsr sfgafhltgk klmntsvdrl
      361 fevgdvfsts ctdirtgtlp dllkeeeeen kdgnekgcsd gdksqywref qrlrrnvhhn
      421 veassaappa appaaslese krtsntlvtv sspspapppa sagattaatp pprtkpavat
      481 pvstsqmpag qsttlkssvk ftmstprtqr tgpahvttvt vdgvarslsd dprssgsype
      541 hqsdssvhsd srsvgslsrs sgqpssagsa rvlintysan hgspnqqqqq qtahatdesr
      601 kpkfvqvtnl edaqvvracd fhpsgqfyav gsnsktlric gyptitdlre gdvahqptvl
      661 fkrlkhhkgs iyclawnqtg dllatgsndk tiklmrfkse tcaldtggea eltmhdgtir
      721 dlcfvedlsn rssllisgga gdckiyvtdc etatpfqals ghsghvlsly twggamfvsg
      781 sqdrtirfwd lrtrgcvnlv tappasgsgp gspvaavavd psgrmlvsgh edatcmlydi
      841 rggrsiqtfr phssdlrsvr fsprayyllt asydrkcvlt dlqgdltqpl psvvvaehad
      901 kviqarwhps dfsfvstsad ktavlwalpv n
//
LOCUS       XP_077506051             931 aa            linear   INV 25-NOV-2025
DEFINITION  WD repeat-containing protein 47-like [Amblyomma americanum].
ACCESSION   XP_077506051
VERSION     XP_077506051.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649925.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..931
                     /product="WD repeat-containing protein 47-like"
                     /calculated_mol_wt=101376
     Region          45..94
                     /region_name="CTLH"
                     /note="C-terminal to LisH motif; smart00668"
                     /db_xref="CDD:128914"
     Region          507..929
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          618..666
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          671..714
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          720..761
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          766..801
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          815..850
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          856..894
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          902..928
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..931
                     /gene="LOC144115515"
                     /coded_by="XM_077649925.1:59..2854"
                     /db_xref="GeneID:144115515"
ORIGIN      
        1 maahlcleea evvrlvlefl anrelsisql aleresgvin dalsddllfl rqlildgqwd
       61 nvldfvqple dmeafdskrf kfsvlkhkfl ellcirseag lvqsaelgvd dmvaclgale
      121 qcspsrehyn qlcllltfpr lcdhldykhw npsnarincf revlplvekl lpapvkspvs
      181 vqrtssgdrl vqllikgily escvnlcqkk atlppcstrd eefhfaellg nadadlslfs
      241 wlqclppevf sypfeqkvln vdirqlvkpf leaswteqil atpikprvfp hsavprtpms
      301 rslimasmeg glfsagshpa lsggakmals madltssmsr sfgafhltgk klmntsvdrl
      361 fevgdvfsts ctdirtgtlp dllkeeeeen kdgnekgcsd gdksqywref qrlrrnvhhn
      421 veassaappa appaaslese krtsntlvtv sspspapppa sagattaatp pprtkpavat
      481 pvstsqmpag qsttlkssvk ftmstprtqr tgpahvttvt vdgvarslsd dprssgsype
      541 hqsdssvhsd srsvgslsrs sgqpssagsa rvlintysan hgspnqqqqq qtahatdesr
      601 kpkfvqvtnl edaqvvracd fhpsgqfyav gsnsktlric gyptitdlre gdvahqptvl
      661 fkrlkhhkgs iyclawnqtg dllatgsndk tiklmrfkse tcaldtggea eltmhdgtir
      721 dlcfvedlsn rssllisgga gdckiyvtdc etatpfqals ghsghvlsly twggamfvsg
      781 sqdrtirfwd lrtrgcvnlv tappasgsgp gspvaavavd psgrmlvsgh edatcmlydi
      841 rggrsiqtfr phssdlrsvr fsprayyllt asydrkcvlt dlqgdltqpl psvvvaehad
      901 kviqarwhps dfsfvstsad ktavlwalpv n
//
LOCUS       XP_077506053             159 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115516 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506053
VERSION     XP_077506053.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649927.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..159
                     /product="uncharacterized protein LOC144115516 isoform X1"
                     /calculated_mol_wt=16300
     CDS             1..159
                     /gene="LOC144115516"
                     /coded_by="XM_077649927.1:536..1015"
                     /db_xref="GeneID:144115516"
ORIGIN      
        1 mpestafert tggglpnpea vlspsdaril givgedcsvg crspavfgma paaavspaps
       61 papqasqvvd ehdycsvqen edqpralatp ptnttsctap arwpnqvaaa vctstsparp
      121 prqcptkaqr drrpnrttra pghnkgpaap adddspgth
//
LOCUS       XP_077506054             159 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115516 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506054
VERSION     XP_077506054.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649928.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..159
                     /product="uncharacterized protein LOC144115516 isoform X1"
                     /calculated_mol_wt=16300
     CDS             1..159
                     /gene="LOC144115516"
                     /coded_by="XM_077649928.1:653..1132"
                     /db_xref="GeneID:144115516"
ORIGIN      
        1 mpestafert tggglpnpea vlspsdaril givgedcsvg crspavfgma paaavspaps
       61 papqasqvvd ehdycsvqen edqpralatp ptnttsctap arwpnqvaaa vctstsparp
      121 prqcptkaqr drrpnrttra pghnkgpaap adddspgth
//
LOCUS       XP_077506055             159 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115516 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506055
VERSION     XP_077506055.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649929.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..159
                     /product="uncharacterized protein LOC144115516 isoform X1"
                     /calculated_mol_wt=16300
     CDS             1..159
                     /gene="LOC144115516"
                     /coded_by="XM_077649929.1:702..1181"
                     /db_xref="GeneID:144115516"
ORIGIN      
        1 mpestafert tggglpnpea vlspsdaril givgedcsvg crspavfgma paaavspaps
       61 papqasqvvd ehdycsvqen edqpralatp ptnttsctap arwpnqvaaa vctstsparp
      121 prqcptkaqr drrpnrttra pghnkgpaap adddspgth
//
LOCUS       XP_077506056              96 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115516 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506056
VERSION     XP_077506056.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649930.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..96
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..96
                     /product="uncharacterized protein LOC144115516 isoform X2"
                     /calculated_mol_wt=9882
     CDS             1..96
                     /gene="LOC144115516"
                     /coded_by="XM_077649930.1:738..1028"
                     /db_xref="GeneID:144115516"
ORIGIN      
        1 mpestafert tggglpnpea vlspsdaril givgedcsvg crspavfgma paaavspaps
       61 papqasqvvd ehdycsvqgk hteithmqrt rtshvr
//
LOCUS       XP_077506057             325 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115517 [Amblyomma americanum].
ACCESSION   XP_077506057
VERSION     XP_077506057.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649931.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..325
                     /product="uncharacterized protein LOC144115517"
                     /calculated_mol_wt=35956
     CDS             1..325
                     /gene="LOC144115517"
                     /coded_by="XM_077649931.1:626..1603"
                     /db_xref="GeneID:144115517"
ORIGIN      
        1 myyhienilh hpqdsgtpdc ivchgahstg srnckfrlar qqptglqqsn edksqtgkee
       61 rrsrprkrss sgergdsksr drsasfpplp gpepgkgggq gsghgrspsa trkkvswpna
      121 gsqnetarek elqrqvlqqa etikkmeari aemeralktg rvdrvqtpla qapqqaaqan
      181 asrvddntam evekvenrgt vkrpppadeg arakrvaets aadtpqpvft vtclkrdmlt
      241 laervgklee rqdrldrrvd kiearlgnie erldaftndm rgfqtqvidm fkqlnamlev
      301 rlppvvgqep mlenlglhhg pspan
//
LOCUS       XP_077506058             325 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115520 [Amblyomma americanum].
ACCESSION   XP_077506058
VERSION     XP_077506058.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649932.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..325
                     /product="uncharacterized protein LOC144115520"
                     /calculated_mol_wt=35926
     Region          <213..>302
                     /region_name="COG4913"
                     /note="Uncharacterized conserved protein, contains a
                     C-terminal ATPase domain [Function unknown]"
                     /db_xref="CDD:443941"
     CDS             1..325
                     /gene="LOC144115520"
                     /coded_by="XM_077649932.1:647..1624"
                     /db_xref="GeneID:144115520"
ORIGIN      
        1 myyhienilh hpqdsgtpdc ivchgahstg srnckfrlar qqptglqqsn edksqtgkee
       61 rrsrprkrss sgargdsksr drsasfpplp gpepgkgggq gsghgrspsa trkkvswpna
      121 gsqnetarek elqrqvlqqa etikkmeari aemeralktg rvdrvqtpla qapqqaaqan
      181 asrvddntam evekvenrgt vkrpppadeg arakrvaets aadtpqpvft vtclkrdmlt
      241 laervgklee rqdrldrrvd riearlgnie erldaftndm rgfqtqvidm fkqlnamlev
      301 rlppvvgqep mlenlglhhg pspan
//
LOCUS       XP_077506059             503 aa            linear   INV 25-NOV-2025
DEFINITION  Y+L amino acid transporter 2-like [Amblyomma americanum].
ACCESSION   XP_077506059
VERSION     XP_077506059.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649933.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..503
                     /product="Y+L amino acid transporter 2-like"
                     /calculated_mol_wt=54725
     Region          35..500
                     /region_name="2A0308"
                     /note="L-type amino acid transporter; TIGR00911"
                     /db_xref="CDD:273332"
     CDS             1..503
                     /gene="LOC144115522"
                     /coded_by="XM_077649933.1:16..1527"
                     /db_xref="GeneID:144115522"
ORIGIN      
        1 maengfqset spllpkgpsi qqdvlpvdrt wtstvelkkr lglsngvsmi vgtivgagif
       61 vspagvirya gsiglalvvw vltglvclis alcytelgtm ipksggdydy ayilaafgpf
      121 paflqlwtml vivkpagnai valtfanyvl rpfyddvdch ppssavmlia alvlclltfi
      181 ncynvkwatf vqdslmfakv ialviiviag fvhlaqgntg nlsnmfegtt tdpgliclsf
      241 yaglfsyagc nylnfvaeel knpfrnlpfa iyislpmvti iyllanisyf vvltadevqs
      301 anavavtfge kvlgvvswim pvsvalstfg glnggifass rlffvgarqg hlpsclamin
      361 vthftpapsl vflcllslly ltntdvfvli tytafseamf imlsvggllw lrikqpntkr
      421 pikvniilpv vfflislflv vlpffsqple tsigagimls gipvyfftiy wkdkplayrk
      481 tiyvvteyvq rilysgpqed kvg
//
LOCUS       XP_077506062             287 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115527 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506062
VERSION     XP_077506062.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649936.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..287
                     /product="uncharacterized protein LOC144115527 isoform X1"
                     /calculated_mol_wt=31015
     CDS             1..287
                     /gene="LOC144115527"
                     /coded_by="XM_077649936.1:479..1342"
                     /db_xref="GeneID:144115527"
ORIGIN      
        1 mqsqrpetly ksspllrapg kgelhphtgi dsgrgnpgls fdrgtaafcs sgqpqtfiev
       61 prgtpsprms serreplvrd ghtwrasgrs lrlgpclptp spgvpgcwpp pptapgfpee
      121 rascedlrhr lnnrrarpld fhcgqgntgg spqenrsgsd isqrsfclrs relsrdtaps
      181 qntrrarpae svgqadnlgr krartsarsd ftdggscrnv qmsmpndeaw svaiaeavsl
      241 wrkqasckdf seqepnetas gkqasgqgva laakeknqql psaeady
//
LOCUS       XP_077506063             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115527 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506063
VERSION     XP_077506063.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649937.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144115527 isoform X2"
                     /calculated_mol_wt=15011
     CDS             1..133
                     /gene="LOC144115527"
                     /coded_by="XM_077649937.1:210..611"
                     /db_xref="GeneID:144115527"
ORIGIN      
        1 mallagltps yrtnvifaac esnlmehscl kgtghfdrss gcescrygfm asktgggrls
       61 sctkgfhlcy cqqnwlrrps flkpcktqgv lsydwrtddk acvqdcpvac klvqyiiwey
      121 ltdfferrik shl
//
LOCUS       XP_077506064             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115527 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506064
VERSION     XP_077506064.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649938.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144115527 isoform X2"
                     /calculated_mol_wt=15011
     CDS             1..133
                     /gene="LOC144115527"
                     /coded_by="XM_077649938.1:269..670"
                     /db_xref="GeneID:144115527"
ORIGIN      
        1 mallagltps yrtnvifaac esnlmehscl kgtghfdrss gcescrygfm asktgggrls
       61 sctkgfhlcy cqqnwlrrps flkpcktqgv lsydwrtddk acvqdcpvac klvqyiiwey
      121 ltdfferrik shl
//
LOCUS       XP_077506065             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115527 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506065
VERSION     XP_077506065.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649939.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144115527 isoform X2"
                     /calculated_mol_wt=15011
     CDS             1..133
                     /gene="LOC144115527"
                     /coded_by="XM_077649939.1:290..691"
                     /db_xref="GeneID:144115527"
ORIGIN      
        1 mallagltps yrtnvifaac esnlmehscl kgtghfdrss gcescrygfm asktgggrls
       61 sctkgfhlcy cqqnwlrrps flkpcktqgv lsydwrtddk acvqdcpvac klvqyiiwey
      121 ltdfferrik shl
//
LOCUS       XP_077506066             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115527 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506066
VERSION     XP_077506066.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649940.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144115527 isoform X2"
                     /calculated_mol_wt=15011
     CDS             1..133
                     /gene="LOC144115527"
                     /coded_by="XM_077649940.1:260..661"
                     /db_xref="GeneID:144115527"
ORIGIN      
        1 mallagltps yrtnvifaac esnlmehscl kgtghfdrss gcescrygfm asktgggrls
       61 sctkgfhlcy cqqnwlrrps flkpcktqgv lsydwrtddk acvqdcpvac klvqyiiwey
      121 ltdfferrik shl
//
LOCUS       XP_077506067             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115527 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506067
VERSION     XP_077506067.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649941.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144115527 isoform X2"
                     /calculated_mol_wt=15011
     CDS             1..133
                     /gene="LOC144115527"
                     /coded_by="XM_077649941.1:216..617"
                     /db_xref="GeneID:144115527"
ORIGIN      
        1 mallagltps yrtnvifaac esnlmehscl kgtghfdrss gcescrygfm asktgggrls
       61 sctkgfhlcy cqqnwlrrps flkpcktqgv lsydwrtddk acvqdcpvac klvqyiiwey
      121 ltdfferrik shl
//
LOCUS       XP_077506068             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115527 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506068
VERSION     XP_077506068.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649942.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144115527 isoform X2"
                     /calculated_mol_wt=15011
     CDS             1..133
                     /gene="LOC144115527"
                     /coded_by="XM_077649942.1:530..931"
                     /db_xref="GeneID:144115527"
ORIGIN      
        1 mallagltps yrtnvifaac esnlmehscl kgtghfdrss gcescrygfm asktgggrls
       61 sctkgfhlcy cqqnwlrrps flkpcktqgv lsydwrtddk acvqdcpvac klvqyiiwey
      121 ltdfferrik shl
//
LOCUS       XP_077506069             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115527 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506069
VERSION     XP_077506069.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649943.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144115527 isoform X2"
                     /calculated_mol_wt=15011
     CDS             1..133
                     /gene="LOC144115527"
                     /coded_by="XM_077649943.1:360..761"
                     /db_xref="GeneID:144115527"
ORIGIN      
        1 mallagltps yrtnvifaac esnlmehscl kgtghfdrss gcescrygfm asktgggrls
       61 sctkgfhlcy cqqnwlrrps flkpcktqgv lsydwrtddk acvqdcpvac klvqyiiwey
      121 ltdfferrik shl
//
LOCUS       XP_077506070             131 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115527 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077506070
VERSION     XP_077506070.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649944.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..131
                     /product="uncharacterized protein LOC144115527 isoform X3"
                     /calculated_mol_wt=14811
     CDS             1..131
                     /gene="LOC144115527"
                     /coded_by="XM_077649944.1:530..925"
                     /db_xref="GeneID:144115527"
ORIGIN      
        1 mallaapsyr tnvifaaces nlmehsclkg tghfdrssgc escrygfmas ktgggrlssc
       61 tkgfhlcycq qnwlrrpsfl kpcktqgvls ydwrtddkac vqdcpvackl vqyiiweylt
      121 dfferriksh l
//
LOCUS       XP_077506072             289 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115530 [Amblyomma americanum].
ACCESSION   XP_077506072
VERSION     XP_077506072.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649946.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..289
                     /product="uncharacterized protein LOC144115530"
                     /calculated_mol_wt=32213
     CDS             1..289
                     /gene="LOC144115530"
                     /coded_by="XM_077649946.1:3077..3946"
                     /db_xref="GeneID:144115530"
ORIGIN      
        1 midgesliqg gfylkklfei qsqllvkpvr fltrthvepn ratqifsapt iatlqflqen
       61 pnchpdagqf kdssatisfm rmvakwyalh digavkargg kheepffvtd derlswleld
      121 ficyledlmm nggrskrmtk etyevtlltt rstvalieyl ldhinfhvlt rglnsdpves
      181 lfrclrqfng sndrvdarta vftaekllkv gilqatrssn aptsfemnap lrlaisdaet
      241 salpagvefa trefyselgf ltpcseadle vapvaflagc varackekv
//
LOCUS       XP_077506073             157 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115531 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506073
VERSION     XP_077506073.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649947.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..157
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..157
                     /product="uncharacterized protein LOC144115531 isoform X1"
                     /calculated_mol_wt=17046
     CDS             1..157
                     /gene="LOC144115531"
                     /coded_by="XM_077649947.1:703..1176"
                     /db_xref="GeneID:144115531"
ORIGIN      
        1 mtasfgtsrr taltfleita rrvrpgplvl llcvtptrsk phrshwrrss stsascsssg
       61 iikapircir wflfaaavrp rvprvagaas srvpsctpav pwcwatrgac isvglhlprn
      121 fsgvrtchnn qydavmfgvl lstraslchl plfeqat
//
LOCUS       XP_077506074             157 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115531 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506074
VERSION     XP_077506074.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649948.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..157
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..157
                     /product="uncharacterized protein LOC144115531 isoform X1"
                     /calculated_mol_wt=17046
     CDS             1..157
                     /gene="LOC144115531"
                     /coded_by="XM_077649948.1:704..1177"
                     /db_xref="GeneID:144115531"
ORIGIN      
        1 mtasfgtsrr taltfleita rrvrpgplvl llcvtptrsk phrshwrrss stsascsssg
       61 iikapircir wflfaaavrp rvprvagaas srvpsctpav pwcwatrgac isvglhlprn
      121 fsgvrtchnn qydavmfgvl lstraslchl plfeqat
//
LOCUS       XP_077506075             157 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115531 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506075
VERSION     XP_077506075.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649949.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..157
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..157
                     /product="uncharacterized protein LOC144115531 isoform X1"
                     /calculated_mol_wt=17046
     CDS             1..157
                     /gene="LOC144115531"
                     /coded_by="XM_077649949.1:704..1177"
                     /db_xref="GeneID:144115531"
ORIGIN      
        1 mtasfgtsrr taltfleita rrvrpgplvl llcvtptrsk phrshwrrss stsascsssg
       61 iikapircir wflfaaavrp rvprvagaas srvpsctpav pwcwatrgac isvglhlprn
      121 fsgvrtchnn qydavmfgvl lstraslchl plfeqat
//
LOCUS       XP_077506076             157 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115531 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506076
VERSION     XP_077506076.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649950.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..157
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..157
                     /product="uncharacterized protein LOC144115531 isoform X1"
                     /calculated_mol_wt=17046
     CDS             1..157
                     /gene="LOC144115531"
                     /coded_by="XM_077649950.1:704..1177"
                     /db_xref="GeneID:144115531"
ORIGIN      
        1 mtasfgtsrr taltfleita rrvrpgplvl llcvtptrsk phrshwrrss stsascsssg
       61 iikapircir wflfaaavrp rvprvagaas srvpsctpav pwcwatrgac isvglhlprn
      121 fsgvrtchnn qydavmfgvl lstraslchl plfeqat
//
LOCUS       XP_077506077             156 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115531 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506077
VERSION     XP_077506077.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649951.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..156
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..156
                     /product="uncharacterized protein LOC144115531 isoform X2"
                     /calculated_mol_wt=16975
     CDS             1..156
                     /gene="LOC144115531"
                     /coded_by="XM_077649951.1:702..1172"
                     /db_xref="GeneID:144115531"
ORIGIN      
        1 mtasfgtsrr taltfleita rrvrpgplvl llcvtptrsk phrshwrrss stsascsssg
       61 iikapircir wflfaavrpr vprvagaass rvpsctpavp wcwatrgaci svglhlprnf
      121 sgvrtchnnq ydavmfgvll straslchlp lfeqat
//
LOCUS       XP_077506078             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115531 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077506078
VERSION     XP_077506078.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649952.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144115531 isoform X3"
                     /calculated_mol_wt=15790
     CDS             1..141
                     /gene="LOC144115531"
                     /coded_by="XM_077649952.1:705..1130"
                     /db_xref="GeneID:144115531"
ORIGIN      
        1 mtasfgtsrr taltfleita rrvrpgplvl llcvtptrsk phrshwrgse llglacvfra
       61 rfyhdhselg legmksghrv pqqlenpcst gmtlvrllsr aifgqwakfs anqeperfsg
      121 prltaanhhl mqpqlvhvrl v
//
LOCUS       XP_077506079             140 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115531 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077506079
VERSION     XP_077506079.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649953.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..140
                     /product="uncharacterized protein LOC144115531 isoform X4"
                     /calculated_mol_wt=15375
     CDS             1..140
                     /gene="LOC144115531"
                     /coded_by="XM_077649953.1:707..1129"
                     /db_xref="GeneID:144115531"
ORIGIN      
        1 mtasfgtsrr taltfleita rrvrgsellg lacvfrarfy hdhselgleg mksghrvpqq
       61 lenpcstgmt lvrllsraif gqwakfsanq eperfsgprl taanhhlmqf rswsppalec
      121 tltgqlglph rgdigvagkl
//
LOCUS       XP_077506080             134 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115531 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077506080
VERSION     XP_077506080.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649954.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..134
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..134
                     /product="uncharacterized protein LOC144115531 isoform X5"
                     /calculated_mol_wt=14413
     CDS             1..134
                     /gene="LOC144115531"
                     /coded_by="XM_077649954.1:705..1109"
                     /db_xref="GeneID:144115531"
ORIGIN      
        1 mtasfgtsrr taltfleita rrvrrsssts ascsssgiik apircirwfl faaavrprvp
       61 rvagaassrv psctpavpwc watrgacisv glhlprnfsg vrtchnnqyd avmfgvllst
      121 raslchlplf eqat
//
LOCUS       XP_077506081             134 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115531 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077506081
VERSION     XP_077506081.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649955.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..134
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..134
                     /product="uncharacterized protein LOC144115531 isoform X6"
                     /calculated_mol_wt=14442
     CDS             1..134
                     /gene="LOC144115531"
                     /coded_by="XM_077649955.1:706..1110"
                     /db_xref="GeneID:144115531"
ORIGIN      
        1 mtasfgtsrr taltfleita rrvrsrsssa cascsrsgiv qgavvyprrp lvlghswglh
       61 lcgatpaaql qrgsdvpqqp vrcghvrgaa vdeglfvppp afraghlkdc ieqriahfpw
      121 kcvtitecfy lpvl
//
LOCUS       XP_077506082             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115531 isoform X7 [Amblyomma
            americanum].
ACCESSION   XP_077506082
VERSION     XP_077506082.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649956.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144115531 isoform X7"
                     /calculated_mol_wt=14342
     CDS             1..133
                     /gene="LOC144115531"
                     /coded_by="XM_077649956.1:705..1106"
                     /db_xref="GeneID:144115531"
ORIGIN      
        1 mtasfgtsrr taltfleita rrvrrsssts ascsssgiik apircirwfl faavrprvpr
       61 vagaassrvp sctpavpwcw atrgacisvg lhlprnfsgv rtchnnqyda vmfgvllstr
      121 aslchlplfe qat
//
LOCUS       XP_077506083             121 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115531 isoform X8 [Amblyomma
            americanum].
ACCESSION   XP_077506083
VERSION     XP_077506083.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649957.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..121
                     /product="uncharacterized protein LOC144115531 isoform X8"
                     /calculated_mol_wt=12828
     CDS             1..121
                     /gene="LOC144115531"
                     /coded_by="XM_077649957.1:598..963"
                     /db_xref="GeneID:144115531"
ORIGIN      
        1 mavsvllhvi vaqepccrqh easymctcah dcklrdfaph rphfsgnnsp pstarpagpl
       61 tvchadaeqa pqvalavgnn mapemdkhfs wvawcpmdll laalvssaay pstmmgglsn
      121 v
//
LOCUS       XP_077506084             114 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115531 isoform X9 [Amblyomma
            americanum].
ACCESSION   XP_077506084
VERSION     XP_077506084.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649958.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..114
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..114
                     /product="uncharacterized protein LOC144115531 isoform X9"
                     /calculated_mol_wt=12597
     CDS             1..114
                     /gene="LOC144115531"
                     /coded_by="XM_077649958.1:701..1045"
                     /db_xref="GeneID:144115531"
ORIGIN      
        1 mtasfgtsrr taltfleita rrvrpgplvl llcvtptrsk phrshwrlls raifgqwakf
       61 sanqeperfs gprltaanhh lmqfrswspp alectltgql glphrgdigv agkl
//
LOCUS       XP_077506085             110 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115531 isoform X10 [Amblyomma
            americanum].
ACCESSION   XP_077506085
VERSION     XP_077506085.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649959.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..110
                     /product="uncharacterized protein LOC144115531 isoform
                     X10"
                     /calculated_mol_wt=11894
     CDS             1..110
                     /gene="LOC144115531"
                     /coded_by="XM_077649959.1:596..928"
                     /db_xref="GeneID:144115531"
ORIGIN      
        1 mavsvllhvi vaqepccrqh easymctcah dcklrdfaph rphfsgnnsp pstarpagpl
       61 tvchadaeqa pqvalavgnn mvlaqghpse cpqkhhepvm vklaqwpqwc
//
LOCUS       XP_077506086             370 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115534 [Amblyomma americanum].
ACCESSION   XP_077506086
VERSION     XP_077506086.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649960.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..370
                     /product="uncharacterized protein LOC144115534"
                     /calculated_mol_wt=40347
     CDS             1..370
                     /gene="LOC144115534"
                     /coded_by="XM_077649960.1:170..1282"
                     /db_xref="GeneID:144115534"
ORIGIN      
        1 matftqiihf sliativvvg sraaqdvlke dgnyhkynir langtsfeit tngfqefskc
       61 lqrgvtrenn sveivrfnkc ardlydqvqt nitdhlrird vgelgaalrn lsslhrlngn
      121 ilknlealqh sfqrlrlppa gsgvdplqrt lqsasdafrn msksvtsgaa lqtvvhnfnr
      181 tlqtfnnlat agvnamtpta nqlmhrfqed lrniqnvtrs lprtdvarlt eqtmgnltll
      241 vrnltaalpv vmrrvatqga essrplrefv tvlsqnlrna sfalssgngg grnstsgalp
      301 saghdllqay dgfqlavnng mlamsdlvrg slklvnpanw nvgrvglgge gqtppvgink
      361 figqlfrrrg
//
LOCUS       XP_077506087             137 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115535 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506087
VERSION     XP_077506087.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649961.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..137
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..137
                     /product="uncharacterized protein LOC144115535 isoform X1"
                     /calculated_mol_wt=14670
     CDS             1..137
                     /gene="LOC144115535"
                     /coded_by="XM_077649961.1:408..821"
                     /db_xref="GeneID:144115535"
ORIGIN      
        1 mglggpavai vvssfgtaft lqvascrrpl qfpafgvasp kcnssrgail rgglqplrrp
       61 hcppsafarv rhlprpprrg hddylrsapv irrllellfp amgvasfkte ltpaadtdcv
      121 egclydflge pifridg
//
LOCUS       XP_077506088             137 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115535 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506088
VERSION     XP_077506088.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649962.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..137
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..137
                     /product="uncharacterized protein LOC144115535 isoform X1"
                     /calculated_mol_wt=14670
     CDS             1..137
                     /gene="LOC144115535"
                     /coded_by="XM_077649962.1:279..692"
                     /db_xref="GeneID:144115535"
ORIGIN      
        1 mglggpavai vvssfgtaft lqvascrrpl qfpafgvasp kcnssrgail rgglqplrrp
       61 hcppsafarv rhlprpprrg hddylrsapv irrllellfp amgvasfkte ltpaadtdcv
      121 egclydflge pifridg
//
LOCUS       XP_077506089             137 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115535 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506089
VERSION     XP_077506089.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649963.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..137
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..137
                     /product="uncharacterized protein LOC144115535 isoform X1"
                     /calculated_mol_wt=14670
     CDS             1..137
                     /gene="LOC144115535"
                     /coded_by="XM_077649963.1:254..667"
                     /db_xref="GeneID:144115535"
ORIGIN      
        1 mglggpavai vvssfgtaft lqvascrrpl qfpafgvasp kcnssrgail rgglqplrrp
       61 hcppsafarv rhlprpprrg hddylrsapv irrllellfp amgvasfkte ltpaadtdcv
      121 egclydflge pifridg
//
LOCUS       XP_077506090             135 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115535 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506090
VERSION     XP_077506090.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649964.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..135
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..135
                     /product="uncharacterized protein LOC144115535 isoform X2"
                     /calculated_mol_wt=14819
     CDS             1..135
                     /gene="LOC144115535"
                     /coded_by="XM_077649964.1:452..859"
                     /db_xref="GeneID:144115535"
ORIGIN      
        1 mlrpehmves elryvdttrt fvsefrrpqv hggpladgdi sskarahtdl qrecglcqrp
       61 aafdpppslp hrplqehrgy lctspvpsra agprqqwsst gptgtswasp psaltsgnpq
      121 rpqmlalkac grrrr
//
LOCUS       XP_077506091             135 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115535 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506091
VERSION     XP_077506091.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649965.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..135
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..135
                     /product="uncharacterized protein LOC144115535 isoform X2"
                     /calculated_mol_wt=14819
     CDS             1..135
                     /gene="LOC144115535"
                     /coded_by="XM_077649965.1:452..859"
                     /db_xref="GeneID:144115535"
ORIGIN      
        1 mlrpehmves elryvdttrt fvsefrrpqv hggpladgdi sskarahtdl qrecglcqrp
       61 aafdpppslp hrplqehrgy lctspvpsra agprqqwsst gptgtswasp psaltsgnpq
      121 rpqmlalkac grrrr
//
LOCUS       XP_077506093             974 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115538 [Amblyomma americanum].
ACCESSION   XP_077506093
VERSION     XP_077506093.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649967.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..974
                     /product="uncharacterized protein LOC144115538"
                     /calculated_mol_wt=108115
     Region          182..>233
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     Region          294..374
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(299,301,303,329..331,367)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133151"
     Site            299..301
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133151"
     Site            299
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133151"
     Site            order(329..332,335..338)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133151"
     Region          <439..500
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     Region          589..638
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          <661..764
                     /region_name="transpos_IS481"
                     /note="IS481 family transposase; NF033577"
                     /db_xref="CDD:468094"
     CDS             1..974
                     /gene="LOC144115538"
                     /coded_by="XM_077649967.1:273..3197"
                     /db_xref="GeneID:144115538"
ORIGIN      
        1 mtslqlltfd dtsdkwkayl iraeayfean aitdskqqra llvaalstqt vqvlagriap
       61 rtpnsltyde avaalnsyyd pkrheiaesy kfftrgqqeg eavraflvei rriadgcnfg
      121 saldrmlrdr ivcgvrstnl rkqllakkdl tleeaealai aaetaendan disgapptvl
      181 rmqashrsss rdtsgaaqec grcgstkhdd nacrwanarc frcrqrghla kkcyrartde
      241 naarrtasvk tltvetasrn enkdeahiwt lisgrkngle pplrrtfrwg eidldmevdt
      301 gspvcvisrn vydrhraqwp rlksphvkls cyagqlpvlg elelpvqfkn vsvtcplvvl
      361 dcsgpslcgr dliaalgktg vtmeeltapd sttgntpsdh mvnrllaeyk dvfsidqgli
      421 kgppaslklk etatpkfcrq ftlvtdhqpl lgllrpdrqt ppmaaariqr walflggyqy
      481 klqyvpgkql ltadalsrlp aptmepgtdg eppeyvlsle sleegivtth elqeltakdp
      541 mlasvthfil hgwpqttkgm apdllpffdr klelslahrl iywgnrvvip rkareqmlql
      601 lhethqgsss mksvarslfw wpgldrdiqn lsaqcrncvq slpmptaapp vswpktavrw
      661 srihidvagp lagkmilivv dahtkwieav plqhantast irclrsmfcr fgvprtivsd
      721 ngtqftsqef atfisknniv hlrtapfhpq sngaaeravr tvkdglrkmg dgqlednlmr
      781 llfnyrrtaq regrspseml lgyqlrsrld tclppravds ssdahdwtis pgssvyvrny
      841 gtgkrwvpgr iqttsgarmv tvntptgavq rhvdqvrrrq eftpgtasve ataraqlegg
      901 pdqvtsadls pvhtpsttgs eqeqspqpla adqsevpspa iterpvvpag ltdplqptlr
      961 rstrirkpvq rfhf
//
LOCUS       XP_077506094             686 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115539 [Amblyomma americanum].
ACCESSION   XP_077506094
VERSION     XP_077506094.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649968.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 16% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..686
                     /product="uncharacterized protein LOC144115539"
                     /calculated_mol_wt=76350
     Region          459..515
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          535..631
                     /region_name="rve"
                     /note="Integrase core domain; pfam00665"
                     /db_xref="CDD:459897"
     CDS             1..686
                     /gene="LOC144115539"
                     /coded_by="XM_077649968.1:1..2061"
                     /db_xref="GeneID:144115539"
ORIGIN      
        1 mrrarewvlq ikfesvecsl vaynvpkehw aqlvfpvlae rvaylstrft aeqhrdydel
       61 kavvldvlkl saaeyqrrfs gatkrreetw kafvtrvqsy lnfylksrcv ssfdgllkll
      121 vadqlkagls eeahkyvklr egekwlnvde iaallqtyee aagkgsaqrk adsfkgfgtp
      181 pkprteappt rkhgaergdr icpfdrenpa drrenlprdd rltarvstha lqstrpalrd
      241 ikicsreavi gaivdtgadi tvvreslvpe efvsphgtid lvsafgekve aklavvplal
      301 crgaplienv ddatpvlctl tdkltqadcl isadaweqlh dsgktdsesl vqavgtlspp
      361 aedrpdrsgp dvgtepvlaf adetlpqqtv dsnevepsqa ggtaelsgaq kfraeqhede
      421 tlrqawqnak qgkagmsivd gvlyhkdqvl gqgvqqlvlp rlrreaalsl ahesywggql
      481 gfrktkarlk ysfywpgmes dirkhcdsch scqvrsdhrr tdrvpitplt rpkfpfqkvn
      541 adiigpieps sgrghryalc iidlytrwpe vvclrslsar atcdallsvf srtgvpevvc
      601 sdcgtnftaa ltkeflerlg ctprfsapnh pesngaverw nrvfktmlsh qlhglggmaa
      661 pqatrnmles fmtpevvlrv ghynih
//
LOCUS       XP_077506095             736 aa            linear   INV 25-NOV-2025
DEFINITION  P-element somatic inhibitor isoform X1 [Amblyomma americanum].
ACCESSION   XP_077506095
VERSION     XP_077506095.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649969.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..736
                     /product="P-element somatic inhibitor isoform X1"
                     /calculated_mol_wt=76395
     Region          102..167
                     /region_name="KH-I_FUBP_rpt1"
                     /note="first type I K homology (KH) RNA-binding domain
                     found in the FUBP family RNA/DNA-binding proteins;
                     cd22396"
                     /db_xref="CDD:411824"
     Site            order(109,111..113,115..119,122..123,126..127,132..135)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411824"
     Region          182..250
                     /region_name="KH-I_FUBP_rpt2"
                     /note="second type I K homology (KH) RNA-binding domain
                     found in the FUBP family RNA/DNA-binding proteins;
                     cd22397"
                     /db_xref="CDD:411825"
     Site            order(190,192..194,196..200,203..204,207..208,213..216)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411825"
     Region          288..355
                     /region_name="KH-I_FUBP_rpt3"
                     /note="third type I K homology (KH) RNA-binding domain
                     found in the FUBP family RNA/DNA-binding proteins;
                     cd22398"
                     /db_xref="CDD:411826"
     Site            order(295..300,302..306,309..310,313,319..322,333)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:411826"
     Site            order(295..300,302..306,309..310,319,321..322,333)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411826"
     Region          422..488
                     /region_name="KH-I_FUBP_rpt4"
                     /note="fourth type I K homology (KH) RNA-binding domain
                     found in the FUBP family RNA/DNA-binding proteins;
                     cd22399"
                     /db_xref="CDD:411827"
     Site            order(429..430,432..434,436..437,439..440,443..444,448,
                     453,455..456,458,467)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411827"
     Region          <615..639
                     /region_name="DUF1897"
                     /note="Domain of unknown function (DUF1897); pfam09005"
                     /db_xref="CDD:462653"
     CDS             1..736
                     /gene="Psi"
                     /coded_by="XM_077649969.1:72..2282"
                     /db_xref="GeneID:144115540"
ORIGIN      
        1 msdftavapp qsnissgydp saafadavqr arqiaakinp psnseagaip gggtkrpled
       61 smdrndgspd akklaavndp fgaqlaamaq qrglanaapa veewsvpdkm vgliigrgge
      121 qisrlqaesg ckiqmapdcg gmserpctlt gprhaiekak dminqiisrg gdpsqlndgh
      181 vvvelmvpgp rvglvigkgg etirglqera nvkmvmiqdg pqqsmmdkpl ritgekskce
      241 yakrlvldli tekelenvrr gfggggpggg eyggppgggp gggpggasqe vlvpkqavgv
      301 vigkqgdmik riqqetgarv qfqqpqddna pdrvclltgg pdqvhhaasf igeliqsvln
      361 rsgfaphrqf gfpppfardq qnmgrgrgrg rggfdggfgp pggpgmgrgg rgrgpggddv
      421 hevqytvpan kcglvigkgg eairqinqqs cahvelsrap ppnpvekvfi irgnpqqieh
      481 aqqlinerig gpppgaangq gppnyptqyp qpyqqppppn qpyapqgwgn ayqhwqnqsa
      541 ppndpskaaa danaaawaay yaqyygqqgg qppqtpqppq agatsampnq aapqqqqqqq
      601 qqppqagapa gaqpdysqaw ieyyrslgmf readmieqqt rgnqnqqgqq gaftpgsagp
      661 ggqqqpgqaa gaapgqpsga gapppaqgyp ysgqqgppag appaaqpsyg appggaaaat
      721 aqypgysyps yggapq
//
LOCUS       XP_077506096             735 aa            linear   INV 25-NOV-2025
DEFINITION  P-element somatic inhibitor isoform X2 [Amblyomma americanum].
ACCESSION   XP_077506096
VERSION     XP_077506096.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649970.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..735
                     /product="P-element somatic inhibitor isoform X2"
                     /calculated_mol_wt=76232
     Region          102..167
                     /region_name="KH-I_FUBP_rpt1"
                     /note="first type I K homology (KH) RNA-binding domain
                     found in the FUBP family RNA/DNA-binding proteins;
                     cd22396"
                     /db_xref="CDD:411824"
     Site            order(109,111..113,115..119,122..123,126..127,132..135)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411824"
     Region          182..250
                     /region_name="KH-I_FUBP_rpt2"
                     /note="second type I K homology (KH) RNA-binding domain
                     found in the FUBP family RNA/DNA-binding proteins;
                     cd22397"
                     /db_xref="CDD:411825"
     Site            order(190,192..194,196..200,203..204,207..208,213..216)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411825"
     Region          288..355
                     /region_name="KH-I_FUBP_rpt3"
                     /note="third type I K homology (KH) RNA-binding domain
                     found in the FUBP family RNA/DNA-binding proteins;
                     cd22398"
                     /db_xref="CDD:411826"
     Site            order(295..300,302..306,309..310,313,319..322,333)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:411826"
     Site            order(295..300,302..306,309..310,319,321..322,333)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411826"
     Region          422..488
                     /region_name="KH-I_FUBP_rpt4"
                     /note="fourth type I K homology (KH) RNA-binding domain
                     found in the FUBP family RNA/DNA-binding proteins;
                     cd22399"
                     /db_xref="CDD:411827"
     Site            order(429..430,432..434,436..437,439..440,443..444,448,
                     453,455..456,458,467)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411827"
     Region          <614..638
                     /region_name="DUF1897"
                     /note="Domain of unknown function (DUF1897); pfam09005"
                     /db_xref="CDD:462653"
     CDS             1..735
                     /gene="Psi"
                     /coded_by="XM_077649970.1:73..2280"
                     /db_xref="GeneID:144115540"
ORIGIN      
        1 msdftavapp qsnissgydp saafadavqr arqiaakinp psnseagaip gggtkrpled
       61 smdrndgspd akklaavndp fgaqlaamaq qrglanaapa veewsvpdkm vgliigrgge
      121 qisrlqaesg ckiqmapdcg gmserpctlt gprhaiekak dminqiisrg gdpsqlndgh
      181 vvvelmvpgp rvglvigkgg etirglqera nvkmvmiqdg pqqsmmdkpl ritgekskce
      241 yakrlvldli tekelenvrr gfggggpggg eyggppgggp gggpggasqe vlvpkqavgv
      301 vigkqgdmik riqqetgarv qfqqpqddna pdrvclltgg pdqvhhaasf igeliqsvln
      361 rsgfaphrqf gfpppfardq qnmgrgrgrg rggfdggfgp pggpgmgrgg rgrgpggddv
      421 hevqytvpan kcglvigkgg eairqinqqs cahvelsrap ppnpvekvfi irgnpqqieh
      481 aqqlinerig gpppgaangq gppnyptqyp qpqqppppnq pyapqgwgna yqhwqnqsap
      541 pndpskaaad anaaawaayy aqyygqqggq ppqtpqppqa gatsampnqa apqqqqqqqq
      601 qppqagapag aqpdysqawi eyyrslgmfr eadmieqqtr gnqnqqgqqg aftpgsagpg
      661 gqqqpgqaag aapgqpsgag apppaqgypy sgqqgppaga ppaaqpsyga ppggaaaata
      721 qypgysypsy ggapq
//
LOCUS       XP_077506097             719 aa            linear   INV 25-NOV-2025
DEFINITION  P-element somatic inhibitor isoform X3 [Amblyomma americanum].
ACCESSION   XP_077506097
VERSION     XP_077506097.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649971.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..719
                     /product="P-element somatic inhibitor isoform X3"
                     /calculated_mol_wt=74454
     Region          102..167
                     /region_name="KH-I_FUBP_rpt1"
                     /note="first type I K homology (KH) RNA-binding domain
                     found in the FUBP family RNA/DNA-binding proteins;
                     cd22396"
                     /db_xref="CDD:411824"
     Site            order(109,111..113,115..119,122..123,126..127,132..135)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411824"
     Region          182..250
                     /region_name="KH-I_FUBP_rpt2"
                     /note="second type I K homology (KH) RNA-binding domain
                     found in the FUBP family RNA/DNA-binding proteins;
                     cd22397"
                     /db_xref="CDD:411825"
     Site            order(190,192..194,196..200,203..204,207..208,213..216)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411825"
     Region          288..355
                     /region_name="KH-I_FUBP_rpt3"
                     /note="third type I K homology (KH) RNA-binding domain
                     found in the FUBP family RNA/DNA-binding proteins;
                     cd22398"
                     /db_xref="CDD:411826"
     Site            order(295..300,302..306,309..310,313,319..322,333)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:411826"
     Site            order(295..300,302..306,309..310,319,321..322,333)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411826"
     Region          405..471
                     /region_name="KH-I_FUBP_rpt4"
                     /note="fourth type I K homology (KH) RNA-binding domain
                     found in the FUBP family RNA/DNA-binding proteins;
                     cd22399"
                     /db_xref="CDD:411827"
     Site            order(412..413,415..417,419..420,422..423,426..427,431,
                     436,438..439,441,450)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411827"
     Region          <598..622
                     /region_name="DUF1897"
                     /note="Domain of unknown function (DUF1897); pfam09005"
                     /db_xref="CDD:462653"
     CDS             1..719
                     /gene="Psi"
                     /coded_by="XM_077649971.1:71..2230"
                     /db_xref="GeneID:144115540"
ORIGIN      
        1 msdftavapp qsnissgydp saafadavqr arqiaakinp psnseagaip gggtkrpled
       61 smdrndgspd akklaavndp fgaqlaamaq qrglanaapa veewsvpdkm vgliigrgge
      121 qisrlqaesg ckiqmapdcg gmserpctlt gprhaiekak dminqiisrg gdpsqlndgh
      181 vvvelmvpgp rvglvigkgg etirglqera nvkmvmiqdg pqqsmmdkpl ritgekskce
      241 yakrlvldli tekelenvrr gfggggpggg eyggppgggp gggpggasqe vlvpkqavgv
      301 vigkqgdmik riqqetgarv qfqqpqddna pdrvclltgg pdqvhhaasf igeliqsvla
      361 rdqqnmgrgr grgrggfdgg fgppggpgmg rggrgrgpgg ddvhevqytv pankcglvig
      421 kggeairqin qqscahvels rapppnpvek vfiirgnpqq iehaqqline riggpppgaa
      481 ngqgppnypt qypqpyqqpp ppnqpyapqg wgnayqhwqn qsappndpsk aaadanaaaw
      541 aayyaqyygq qggqppqtpq ppqagatsam pnqaapqqqq qqqqqppqag apagaqpdys
      601 qawieyyrsl gmfreadmie qqtrgnqnqq gqqgaftpgs agpggqqqpg qaagaapgqp
      661 sgagapppaq gypysgqqgp pagappaaqp sygappggaa aataqypgys ypsyggapq
//
LOCUS       XP_077506098             364 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115541 [Amblyomma americanum].
ACCESSION   XP_077506098
VERSION     XP_077506098.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649972.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..364
                     /product="uncharacterized protein LOC144115541"
                     /calculated_mol_wt=40342
     Region          13..>149
                     /region_name="DnaQ_like_exo"
                     /note="DnaQ-like (or DEDD) 3'-5' exonuclease domain
                     superfamily; cl10012"
                     /db_xref="CDD:447876"
     Site            order(17..20,111..112,115..117)
                     /site_type="active"
                     /db_xref="CDD:176647"
     Site            order(18..20,111..112,115..116)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:176647"
     CDS             1..364
                     /gene="LOC144115541"
                     /coded_by="XM_077649972.1:593..1687"
                     /db_xref="GeneID:144115541"
ORIGIN      
        1 mdrlpgqrqi etlvfldles tglpdtmprq kvnvielsli avarkhlqlp lrcvhkltyc
       61 vqprnavtcq aahitgldnw elelcplfre vadmiekfly tlqppiclva hngdkfdfpl
      121 lraelkhasp gidlmsffcc dslpafreil gnaadsrevn eltalheldq efwqafdasm
      181 sdeeeqlsrp virpvnstpq rrppgfqgnr sappppkktr pesgmtgshs qlqhpsvakk
      241 elfpsdvgpa tasgsaqhss rtkangnngv shmllecesv lalprsviap psftggsksv
      301 ndkrnirfkl asvyervmgt empvaheaea dcralaeica rlgrpfleyv dakhrmfdsv
      361 apmw
//
LOCUS       XP_077506099             312 aa            linear   INV 25-NOV-2025
DEFINITION  three prime repair exonuclease 2-like [Amblyomma americanum].
ACCESSION   XP_077506099
VERSION     XP_077506099.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649973.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..312
                     /product="three prime repair exonuclease 2-like"
                     /calculated_mol_wt=33736
     Region          90..287
                     /region_name="DnaQ_like_exo"
                     /note="DnaQ-like (or DEDD) 3'-5' exonuclease domain
                     superfamily; cl10012"
                     /db_xref="CDD:447876"
     Site            order(94..97,190..191,194..196,279)
                     /site_type="active"
                     /db_xref="CDD:176647"
     Site            order(94,96,196,279)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:176647"
     Site            order(95..97,190..191,194..195,279)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:176647"
     CDS             1..312
                     /gene="LOC144115544"
                     /coded_by="XM_077649973.1:96..1034"
                     /db_xref="GeneID:144115544"
ORIGIN      
        1 meynmtqvqa ttavvssfsg tfpvamlgas plgplqiqsp drparpaalp mqphgvfgtv
       61 teqtvqkqtt vsavapaqls pvlpepvktl vffdlettgl pssyqrvsit elamvavdra
      121 hfekqdkaef rvtnkltlcl kpvapmhpma vkksglnptl lkdqcyfqka lpavraflss
      181 ltppvcllah ngdpfdfpil mdelkfagns ndvdsilgvs gltccdtlka vrnmwpvlps
      241 apkkgrgsva askqnsyald qlykrfcgrr snahhaeqdc vdlmkvchae kaaflgyvda
      301 katllaeays kw
//
LOCUS       XP_077506100             110 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115547 [Amblyomma americanum].
ACCESSION   XP_077506100
VERSION     XP_077506100.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649974.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..110
                     /product="uncharacterized protein LOC144115547"
                     /calculated_mol_wt=12197
     CDS             1..110
                     /gene="LOC144115547"
                     /coded_by="XM_077649974.1:99..431"
                     /db_xref="GeneID:144115547"
ORIGIN      
        1 mristykecw arkstrttys ealeaagcrg fqavcascgr qasckasqlp cflppartrp
       61 vcpwgryqgq sacsrqrral hllhrssqlw cravrvsrsg argrrkcasc
//
LOCUS       XP_077506101             816 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077506101
VERSION     XP_077506101.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649975.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..816
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=90223
     Region          123..256
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          352..737
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..816
                     /gene="LOC144115548"
                     /coded_by="XM_077649975.1:31..2481"
                     /db_xref="GeneID:144115548"
ORIGIN      
        1 mrtnataqrr hgcspplrvl lrlvpmpsvl plllllaatv mllgdtasad psigdvfrqi
       61 reravateei vapappnktr nerileqwsk felnlqdlvd svirkvlptv irrsselevs
      121 pdcrdallkv vfgirqlkrw afqfldaagk paggiltgtl tafgsydqcv aieardspqe
      181 eprftgryct velrppipnr pryytlhdqv qlfnnttrtk vtgeiaekmh ffyflvyryg
      241 vcvpstcsre dvetiaaslv ksaefevkvs ncevkdengk lnpdqtaivt lvciltvial
      301 astgvdlvkk amrqpdkket eaetllarft tsfsmvrslr svfrpdtslg plsalhglrs
      361 ltllwfmlgh iflfvnyqff rnlmiavgfa kdfsfqivin stlatdtlif lngflfgyhg
      421 lvdhestgrr rqpifsvlhk afravvamll atmvaillpv igsgpmwket mesvaqnchd
      481 nwwlnvlfvn nifvapherc leitwyfavl iqlclvgtav ifvlqrrffv gvvlnaslva
      541 vgiismgvlt atyklpptvl faqadinsrl tmedimyqkp wahlgpfcig iglacivtrk
      601 pvvtlnpilr algwvcsflc avavvfgayr wncgdgvdta qaalyaavhr tvwafavawv
      661 afacvtgkaw lvgdflswpa fgplsrmapl vfllhplvqn iftayvrerv qadqlmalfl
      721 fcglfvlgyl gavlcsvlad aplhsleqlf cerlglsaag regcprrfle vakpvlkalp
      781 lpsctkitil gekahagsds ggggtdanrl ppdsrl
//
LOCUS       XP_077506102             188 aa            linear   INV 25-NOV-2025
DEFINITION  frequenin-1-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077506102
VERSION     XP_077506102.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649976.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..188
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..188
                     /product="frequenin-1-like isoform X1"
                     /calculated_mol_wt=21951
     Region          40..175
                     /region_name="FRQ1"
                     /note="Ca2+-binding protein, EF-hand superfamily [Signal
                     transduction mechanisms]; COG5126"
                     /db_xref="CDD:444056"
     CDS             1..188
                     /gene="LOC144115549"
                     /coded_by="XM_077649976.1:430..996"
                     /db_xref="GeneID:144115549"
ORIGIN      
        1 mgkknsklka erlfklvnet yfsekevkqw ykgflkdcpn gqlteqgflr iykqffpqgd
       61 pskfaslvfr vfdenkdgsi efeefirals vtsrgsldek lvwafklydv dndgfitrde
      121 mynivdaiyq mlgnqtkese qeespqervd kifdqldknh dnrltleefr egskqdpkiv
      181 qalslyap
//
LOCUS       XP_077506103             166 aa            linear   INV 25-NOV-2025
DEFINITION  frequenin-1-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077506103
VERSION     XP_077506103.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649977.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..166
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..166
                     /product="frequenin-1-like isoform X2"
                     /calculated_mol_wt=19270
     Region          <47..153
                     /region_name="FRQ1"
                     /note="Ca2+-binding protein, EF-hand superfamily [Signal
                     transduction mechanisms]; COG5126"
                     /db_xref="CDD:444056"
     CDS             1..166
                     /gene="LOC144115549"
                     /coded_by="XM_077649977.1:9..509"
                     /db_xref="GeneID:144115549"
ORIGIN      
        1 mrplssytps ryvpngiffv wgvkgflriy kqffpqgdps kfaslvfrvf denkdgsief
       61 eefiralsvt srgsldeklv wafklydvdn dgfitrdemy nivdaiyqml gnqtkeseqe
      121 espqervdki fdqldknhdn rltleefreg skqdpkivqa lslyap
//
LOCUS       XP_077506104             456 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115551 [Amblyomma americanum].
ACCESSION   XP_077506104
VERSION     XP_077506104.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649978.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..456
                     /product="uncharacterized protein LOC144115551"
                     /calculated_mol_wt=48148
     CDS             1..456
                     /gene="LOC144115551"
                     /coded_by="XM_077649978.1:1485..2855"
                     /db_xref="GeneID:144115551"
ORIGIN      
        1 mppkpfavvk fpmeddslav vpvgwlshdw qhchwpkrra aevirlaree adpsapgcew
       61 frcpvavvtt crsyknaekk akkyemssav essaisgneg pdgpdvrlps ppprlpsppp
      121 rlpsppprlp spppqlpspp rlisppslls shsmeaatpa aanctagcpa gsastagdle
      181 dveeatsdpt mlkvlrllle irqqqreilq rvsrleqarq eprtrspspp vsslpplcpq
      241 lpafsiedfe aaeaavrddr vaaalkkqll rlggnnlkev aanvmgavmg vavqrlfslr
      301 grkgkrpfvg tklcrvatda icerqgvdil aaqgfigrwl pgacdrgggr krryaqtlep
      361 peshaqappa npcagsapcp gapsascpat ppgmailsps gvhpssappt asssrpatlh
      421 pssapspapp tasssrpatp rstlatwvia prahek
//
LOCUS       XP_077506105             147 aa            linear   INV 25-NOV-2025
DEFINITION  ubiquitin-conjugating enzyme E2-17 kDa [Amblyomma americanum].
ACCESSION   XP_077506105
VERSION     XP_077506105.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649979.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..147
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..147
                     /product="ubiquitin-conjugating enzyme E2-17 kDa"
                     /calculated_mol_wt=16477
     Region          3..145
                     /region_name="UBCc_UBE2D"
                     /note="Ubiquitin-conjugating enzyme E2, catalytic (UBCc)
                     domain of ubiquitin conjugating enzyme E2 D1-D3 and
                     related proteins; cd23792"
                     /db_xref="CDD:467412"
     CDS             1..147
                     /gene="LOC144115556"
                     /coded_by="XM_077649979.1:118..561"
                     /db_xref="GeneID:144115556"
ORIGIN      
        1 malkrinkel qdlgrdppaq csagpvgddl fhwqatimgp pdspyqggvf fltihfptdy
       61 pfkppkvaft triyhpnins ngsicldilr sqwspaltis kvllsicsll cdpnpddplv
      121 peiakiyktd rekyndlake wtrkyam
//
LOCUS       XP_077506107             100 aa            linear   INV 25-NOV-2025
DEFINITION  CDGSH iron sulfur domain 2 [Amblyomma americanum].
ACCESSION   XP_077506107
VERSION     XP_077506107.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649981.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..100
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..100
                     /product="CDGSH iron sulfur domain 2"
                     /calculated_mol_wt=11017
     Region          47..85
                     /region_name="ZnF_CDGSH"
                     /note="CDGSH-type zinc finger. Function unknown;
                     smart00704"
                     /db_xref="CDD:197836"
     CDS             1..100
                     /gene="Cisd2"
                     /coded_by="XM_077649981.1:136..438"
                     /db_xref="GeneID:144115557"
ORIGIN      
        1 mstkesllpf lpwlgtaaal gfavlayrsy skakgrvnlq idmsnpkivn svdiedignk
       61 avfcrcwksk kfpycdgshn khneecsdnv gpliikrkda
//
LOCUS       XP_077506108            3525 aa            linear   INV 25-NOV-2025
DEFINITION  WD repeat and FYVE domain containing 3 bchs [Amblyomma americanum].
ACCESSION   XP_077506108
VERSION     XP_077506108.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649982.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..3525
                     /product="WD repeat and FYVE domain containing 3 bchs"
                     /calculated_mol_wt=393470
     Region          <1161..1226
                     /region_name="Laminin_G_3"
                     /note="Concanavalin A-like lectin/glucanases superfamily;
                     pfam13385"
                     /db_xref="CDD:463865"
     Region          1375..>1528
                     /region_name="DUF4704"
                     /note="Neurobeachin/BDCP, DUF4704 alpha solenoid region;
                     pfam15787"
                     /db_xref="CDD:464870"
     Region          2455..2579
                     /region_name="PH_BEACH"
                     /note="Pleckstrin homology domain in BEACH domain
                     containing proteins; cd01201"
                     /db_xref="CDD:275391"
     Region          2617..2897
                     /region_name="Beach"
                     /note="Beige/BEACH domain; pfam02138"
                     /db_xref="CDD:460459"
     Region          2954..2996
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          2969..>3166
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          3004..3041
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3046..3082
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3089..3122
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3455..3519
                     /region_name="FYVE_WDFY3"
                     /note="FYVE domain found in WD40 repeat and FYVE
                     domain-containing protein 3 (WDFY3) and similar proteins;
                     cd15719"
                     /db_xref="CDD:277259"
     Site            order(3457,3460,3478..3483,3485..3486,3510..3512)
                     /site_type="other"
                     /note="putative phosphatidylinositol 3-phosphate binding
                     site [chemical binding]"
                     /db_xref="CDD:277259"
     CDS             1..3525
                     /gene="bchs"
                     /coded_by="XM_077649982.1:351..10928"
                     /db_xref="GeneID:144115558"
ORIGIN      
        1 mnimrkllvg hqgrgagtsd dgaqssqhta lglmhlhklf aelshpahpl tqqeqeqrly
       61 nmlplfckvf enspaadiid kfsdvlafsk lvsklmvsei rrrasnqste sassaiaqfl
      121 eiesteeqsr gwmllatmnl lasggaplve vmtssalpst lvkclylffd lpqlrdpdrl
      181 ephceftpre rrillqkvfv qvlvrlcshp apaeelarkd dltllfsait twcppynnvw
      241 rksasevlmt lsrhgltapv vnyihskgcv alcvenvqrv qelsplevve mfvtifcflk
      301 dssevsqlll ddfrscqgyv flsefllrle qnssqeacea lrnlavlmts lttcgyyelk
      361 psqastgslf qipgftlpqp sgkgtsvrni qafqvlqtvf pkaqtvhlss llldaistiy
      421 hsdkanyfil esshplshfs ekihlktpei qekffkllef ivmdlkfvpc kelislsill
      481 ktnssiscsi vclhtlanil qhnavfkdvy revgllevlv tclhryatel keafpdgaae
      541 pvakvpipde qqqmgslvme tltvllngns nnasvfrecg garcahnlvp yrlcrqqala
      601 vvqqlvlsng gdddmgtllg lmhtapplal dlknhilksl lfvlreshrt rtvfrkvggf
      661 vyvmsvlvsm egcladppre pwntvekkqi isllrtvfst mtvamryepa nakffateic
      721 qtsladtvrl lgcftnntdi cpvdtsfvpt eenlfhavft gqndssvnlt kvptslisal
      781 vvirllydma ldsfdkpmal tklvtcpptl hctvevpkka pdttgkgspq tkkpvlatln
      841 lsapspdpli vhpgvvvaml hlipsiadgh dsqwlqvelt lqlylaevlr slvrsernqq
      901 vmceaglpge llqrcrpale deahplhlpl qymlerlaaq aleprdlrsf lrlgsplccn
      961 lfdvgseykr qaggsvclvr aqssrltnqk dggpvpltri ktlvsmttpr dcrlhgtllt
     1021 ppfvefemap egfsclylss vapqsasggp pmvgvsmvgg apegviggig tgdrvfppqp
     1081 glsfstwlsv erfsdphvdp havrlltlvr nlhgrddhlv clviqlssrd kallvstqei
     1141 plpnltgadw epevkeegci rfwspellqe gqwhhvvivl nravlknssv siyidgqhms
     1201 tqkipyisqn pgggaanltv assvygfigt ppqfrhpsrl iwkqgpchmm eevmspqiih
     1261 qiyllgpsyv gslqapvmig tdplppflse ekvvfglnat avsvmtlaki rrvysktdck
     1321 siakmlgmss henatpiril hnsaahlsgp arclggvlig ylgartfipr pvaltienig
     1381 gvavllglva maqdveglya tvkalvcvvr snraaqqemd rirgyqtlam llkkksalln
     1441 shilhlafsl vgtvdsgret tvipnmtafe dllcslevwr dapgdlhksl yehfyelite
     1501 sseqkanlri lrdlemvtrv lmmlrdpylt pstirllcnl lktllqntnr sgdilrfgqf
     1561 mvstlpnsmv sektlnlkrd vesaesaapm detallihhv clrnrclhil hlmtyqgsks
     1621 vnvpfceela rvlgmdwlll fmqshlhptt vvlalqcltv llscpslmqr freashnggw
     1681 ltetdlvlqn rttvllgfnv stannkgsgs qsirmeayqv pgfqqlqwlm cqhvtvpdin
     1741 flllamlmgr pvsripcglq fsldniwsfl fgvppghsim sqgdinfelv vvllamvrci
     1801 lsqprgenvs gveehpvtli qflmylynnr qdfmnmcmss dilcalsatl fpfanglsev
     1861 aspaddfqii snpkcsesna glskhparky vmdflrvivv dslslafptk qapvidlvle
     1921 afpnhatlnq qkefvteils slrehllaad vllddqtalp itaggkysni ppnvfyltsc
     1981 fvdklwqgvy trdpheildf iikllgqvkr kgmhvqtenv yrslnrtllf ilsrptecva
     2041 dqmtkleclq kmiihrtlml vaanseadfi pclcycllql tadaqismet vrrttwhinp
     2101 asfsdrsrni adnkslssse eghllvasaa ckvweivyls krqaleelck vplpaaegss
     2161 ggqipdlqsl wevlheplcr awtsfldler ksgytrevmq tqmqtvsqkl qkvtgglsrl
     2221 asrkikreqa pkvplstmts qevemwtqvh vsivqelvdm qlkrhqqsqq hlqkdvfedw
     2281 lqteteltqe rglwgpvegs lldkwmldmt eagpcrmrkk mvkndlfylh ypyrpdvegg
     2341 enralkykva tsfdskeyyk hcrpdslveh dtfspdvisi tsedwpqegd saeereigfq
     2401 glrtrlqanr svsgdqdeds esldpasdas eqegaarkde spdnqsvlrl lekgekithm
     2461 frcarvqgld tceglllfgk ehfyvvdgft llktreirdi dslpanmhdp iipnssprsk
     2521 hqkrmcskfs yddirevhkr ryllqpiale vfsadgrnyl lvfprkvrnk vytrflsaat
     2581 aitdsaqesl agqrrganve sgasllsnli getsvtqrwl rgeisnfqyl mhlntlagrs
     2641 yndlmqypvf pwvladydse eldlndsatf rdfskpmgaq tsdrldqfkk ryrewddphg
     2701 etppyhygtf yssamivasy lvrmepftqh flrlqgghfd ladrmfhsvk dawlsaskhn
     2761 madvkelipe ffylpeflln snrfdlgckq ngvqldnivl ppwakgdpre firvhrmale
     2821 sdyvsahlhe widlifgykq qgqpaieavn vfhhlfyegn vdiysiddpl kknatigfin
     2881 nfgqipkqlf kkphpckklt nrtsvvdpgp impglsychd klffhnldnl kpslhplkel
     2941 kgpvgqiiqh ektvlaveqn kgligplynr yvawgfadys irmgpyeser cnfvwedvps
     3001 geilccacpd skiiitagts svvtvwqfdk kkltlkqnlh ghteavtcla asrpynlivs
     3061 gsrdyscilw dlsrlvfvrq lrghtapiaa vaindltgdl atcagthitl wtingqevas
     3121 vntatgrsdr lqqilcvcfs qlnewdnqnv imtgssdgvv rmwsadyvqi piegkkpnps
     3181 stavtpavve epcivttpek sssppptqpa pqqetmaeri vrklsavdtd dvdsfrtqht
     3241 sasvlikcgs dsslseddds vksygrgats plsgssgesg wrrssqdrrp sllqhsktvv
     3301 gllstqqqlk aaaeaeaqit kstltipedt rstggsaaed akgaalrisk sensltdtfv
     3361 mineadaldv nksidvrklr rrrnilregf kwscqlvfrs kltmhtafdr kdnnepaavt
     3421 alavskdhkt vyvgdargrv ftwsvtehpg ramadhwvrd eggdsclscg vrfsfaerrh
     3481 hcrncgqlfc srcsrfesei srlrilkpvr vcqtcfnslk ppget
//
LOCUS       XP_077506109            3525 aa            linear   INV 25-NOV-2025
DEFINITION  WD repeat and FYVE domain containing 3 bchs [Amblyomma americanum].
ACCESSION   XP_077506109
VERSION     XP_077506109.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649983.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..3525
                     /product="WD repeat and FYVE domain containing 3 bchs"
                     /calculated_mol_wt=393470
     Region          <1161..1226
                     /region_name="Laminin_G_3"
                     /note="Concanavalin A-like lectin/glucanases superfamily;
                     pfam13385"
                     /db_xref="CDD:463865"
     Region          1375..>1528
                     /region_name="DUF4704"
                     /note="Neurobeachin/BDCP, DUF4704 alpha solenoid region;
                     pfam15787"
                     /db_xref="CDD:464870"
     Region          2455..2579
                     /region_name="PH_BEACH"
                     /note="Pleckstrin homology domain in BEACH domain
                     containing proteins; cd01201"
                     /db_xref="CDD:275391"
     Region          2617..2897
                     /region_name="Beach"
                     /note="Beige/BEACH domain; pfam02138"
                     /db_xref="CDD:460459"
     Region          2954..2996
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          2969..>3166
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          3004..3041
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3046..3082
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3089..3122
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3455..3519
                     /region_name="FYVE_WDFY3"
                     /note="FYVE domain found in WD40 repeat and FYVE
                     domain-containing protein 3 (WDFY3) and similar proteins;
                     cd15719"
                     /db_xref="CDD:277259"
     Site            order(3457,3460,3478..3483,3485..3486,3510..3512)
                     /site_type="other"
                     /note="putative phosphatidylinositol 3-phosphate binding
                     site [chemical binding]"
                     /db_xref="CDD:277259"
     CDS             1..3525
                     /gene="bchs"
                     /coded_by="XM_077649983.1:355..10932"
                     /db_xref="GeneID:144115558"
ORIGIN      
        1 mnimrkllvg hqgrgagtsd dgaqssqhta lglmhlhklf aelshpahpl tqqeqeqrly
       61 nmlplfckvf enspaadiid kfsdvlafsk lvsklmvsei rrrasnqste sassaiaqfl
      121 eiesteeqsr gwmllatmnl lasggaplve vmtssalpst lvkclylffd lpqlrdpdrl
      181 ephceftpre rrillqkvfv qvlvrlcshp apaeelarkd dltllfsait twcppynnvw
      241 rksasevlmt lsrhgltapv vnyihskgcv alcvenvqrv qelsplevve mfvtifcflk
      301 dssevsqlll ddfrscqgyv flsefllrle qnssqeacea lrnlavlmts lttcgyyelk
      361 psqastgslf qipgftlpqp sgkgtsvrni qafqvlqtvf pkaqtvhlss llldaistiy
      421 hsdkanyfil esshplshfs ekihlktpei qekffkllef ivmdlkfvpc kelislsill
      481 ktnssiscsi vclhtlanil qhnavfkdvy revgllevlv tclhryatel keafpdgaae
      541 pvakvpipde qqqmgslvme tltvllngns nnasvfrecg garcahnlvp yrlcrqqala
      601 vvqqlvlsng gdddmgtllg lmhtapplal dlknhilksl lfvlreshrt rtvfrkvggf
      661 vyvmsvlvsm egcladppre pwntvekkqi isllrtvfst mtvamryepa nakffateic
      721 qtsladtvrl lgcftnntdi cpvdtsfvpt eenlfhavft gqndssvnlt kvptslisal
      781 vvirllydma ldsfdkpmal tklvtcpptl hctvevpkka pdttgkgspq tkkpvlatln
      841 lsapspdpli vhpgvvvaml hlipsiadgh dsqwlqvelt lqlylaevlr slvrsernqq
      901 vmceaglpge llqrcrpale deahplhlpl qymlerlaaq aleprdlrsf lrlgsplccn
      961 lfdvgseykr qaggsvclvr aqssrltnqk dggpvpltri ktlvsmttpr dcrlhgtllt
     1021 ppfvefemap egfsclylss vapqsasggp pmvgvsmvgg apegviggig tgdrvfppqp
     1081 glsfstwlsv erfsdphvdp havrlltlvr nlhgrddhlv clviqlssrd kallvstqei
     1141 plpnltgadw epevkeegci rfwspellqe gqwhhvvivl nravlknssv siyidgqhms
     1201 tqkipyisqn pgggaanltv assvygfigt ppqfrhpsrl iwkqgpchmm eevmspqiih
     1261 qiyllgpsyv gslqapvmig tdplppflse ekvvfglnat avsvmtlaki rrvysktdck
     1321 siakmlgmss henatpiril hnsaahlsgp arclggvlig ylgartfipr pvaltienig
     1381 gvavllglva maqdveglya tvkalvcvvr snraaqqemd rirgyqtlam llkkksalln
     1441 shilhlafsl vgtvdsgret tvipnmtafe dllcslevwr dapgdlhksl yehfyelite
     1501 sseqkanlri lrdlemvtrv lmmlrdpylt pstirllcnl lktllqntnr sgdilrfgqf
     1561 mvstlpnsmv sektlnlkrd vesaesaapm detallihhv clrnrclhil hlmtyqgsks
     1621 vnvpfceela rvlgmdwlll fmqshlhptt vvlalqcltv llscpslmqr freashnggw
     1681 ltetdlvlqn rttvllgfnv stannkgsgs qsirmeayqv pgfqqlqwlm cqhvtvpdin
     1741 flllamlmgr pvsripcglq fsldniwsfl fgvppghsim sqgdinfelv vvllamvrci
     1801 lsqprgenvs gveehpvtli qflmylynnr qdfmnmcmss dilcalsatl fpfanglsev
     1861 aspaddfqii snpkcsesna glskhparky vmdflrvivv dslslafptk qapvidlvle
     1921 afpnhatlnq qkefvteils slrehllaad vllddqtalp itaggkysni ppnvfyltsc
     1981 fvdklwqgvy trdpheildf iikllgqvkr kgmhvqtenv yrslnrtllf ilsrptecva
     2041 dqmtkleclq kmiihrtlml vaanseadfi pclcycllql tadaqismet vrrttwhinp
     2101 asfsdrsrni adnkslssse eghllvasaa ckvweivyls krqaleelck vplpaaegss
     2161 ggqipdlqsl wevlheplcr awtsfldler ksgytrevmq tqmqtvsqkl qkvtgglsrl
     2221 asrkikreqa pkvplstmts qevemwtqvh vsivqelvdm qlkrhqqsqq hlqkdvfedw
     2281 lqteteltqe rglwgpvegs lldkwmldmt eagpcrmrkk mvkndlfylh ypyrpdvegg
     2341 enralkykva tsfdskeyyk hcrpdslveh dtfspdvisi tsedwpqegd saeereigfq
     2401 glrtrlqanr svsgdqdeds esldpasdas eqegaarkde spdnqsvlrl lekgekithm
     2461 frcarvqgld tceglllfgk ehfyvvdgft llktreirdi dslpanmhdp iipnssprsk
     2521 hqkrmcskfs yddirevhkr ryllqpiale vfsadgrnyl lvfprkvrnk vytrflsaat
     2581 aitdsaqesl agqrrganve sgasllsnli getsvtqrwl rgeisnfqyl mhlntlagrs
     2641 yndlmqypvf pwvladydse eldlndsatf rdfskpmgaq tsdrldqfkk ryrewddphg
     2701 etppyhygtf yssamivasy lvrmepftqh flrlqgghfd ladrmfhsvk dawlsaskhn
     2761 madvkelipe ffylpeflln snrfdlgckq ngvqldnivl ppwakgdpre firvhrmale
     2821 sdyvsahlhe widlifgykq qgqpaieavn vfhhlfyegn vdiysiddpl kknatigfin
     2881 nfgqipkqlf kkphpckklt nrtsvvdpgp impglsychd klffhnldnl kpslhplkel
     2941 kgpvgqiiqh ektvlaveqn kgligplynr yvawgfadys irmgpyeser cnfvwedvps
     3001 geilccacpd skiiitagts svvtvwqfdk kkltlkqnlh ghteavtcla asrpynlivs
     3061 gsrdyscilw dlsrlvfvrq lrghtapiaa vaindltgdl atcagthitl wtingqevas
     3121 vntatgrsdr lqqilcvcfs qlnewdnqnv imtgssdgvv rmwsadyvqi piegkkpnps
     3181 stavtpavve epcivttpek sssppptqpa pqqetmaeri vrklsavdtd dvdsfrtqht
     3241 sasvlikcgs dsslseddds vksygrgats plsgssgesg wrrssqdrrp sllqhsktvv
     3301 gllstqqqlk aaaeaeaqit kstltipedt rstggsaaed akgaalrisk sensltdtfv
     3361 mineadaldv nksidvrklr rrrnilregf kwscqlvfrs kltmhtafdr kdnnepaavt
     3421 alavskdhkt vyvgdargrv ftwsvtehpg ramadhwvrd eggdsclscg vrfsfaerrh
     3481 hcrncgqlfc srcsrfesei srlrilkpvr vcqtcfnslk ppget
//
LOCUS       XP_077506110             462 aa            linear   INV 25-NOV-2025
DEFINITION  transcription factor pangolin isoform X1 [Amblyomma americanum].
ACCESSION   XP_077506110
VERSION     XP_077506110.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649984.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..462
                     /product="transcription factor pangolin isoform X1"
                     /calculated_mol_wt=50273
     Region          224..302
                     /region_name="HMG-box_TCF7-like"
                     /note="high mobility group (HMG)-box found in the
                     transcription factor 7 (TCF-7)-like family; cd21996"
                     /db_xref="CDD:438812"
     Site            order(224,226..233,236..237,240,250..253,256..257,260,275,
                     279,282,293..302)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438812"
     CDS             1..462
                     /gene="pan"
                     /coded_by="XM_077649984.1:11..1399"
                     /db_xref="GeneID:144115559"
ORIGIN      
        1 mvapphpgsp lnfvvynepf sqpppahmgi ppvhidpktg iprpsvyplt spgqythsmf
       61 sqefaqqlhw hsaaagmypm ppmasggfrg gssyapplpr lnppllppsi mphglhpalv
      121 tpgpkqelss plpdtsmapt fprhplamyh pldsskcnqg rpssrgdlvn gttaglnghg
      181 ggppssqpag hppsngssss sqphqpqqqq hngtekkpqk ptnhvkkpln afmlymkemr
      241 akvvaectlk esaainqilg rrwhslsree qskyyemark erqihmqlyp gwtardnyai
      301 nskkkkrkkd ksqdgdlnnp kkcrarygld qqsdwckpcr rkkkciryqe gldgnanese
      361 dnigsveapt peshtshggg dtttttstgs pctslgtpep spgppvrrpd cyslnkhhpl
      421 svhhltgqpl vkrepddpvd iltppstdss ttpsslpllt vt
//
LOCUS       XP_077506111             455 aa            linear   INV 25-NOV-2025
DEFINITION  transcription factor pangolin isoform X2 [Amblyomma americanum].
ACCESSION   XP_077506111
VERSION     XP_077506111.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649985.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..455
                     /product="transcription factor pangolin isoform X2"
                     /calculated_mol_wt=49541
     Region          217..295
                     /region_name="HMG-box_TCF7-like"
                     /note="high mobility group (HMG)-box found in the
                     transcription factor 7 (TCF-7)-like family; cd21996"
                     /db_xref="CDD:438812"
     Site            order(217,219..226,229..230,233,243..246,249..250,253,268,
                     272,275,286..295)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438812"
     CDS             1..455
                     /gene="pan"
                     /coded_by="XM_077649985.1:11..1378"
                     /db_xref="GeneID:144115559"
ORIGIN      
        1 mvapphpgsp lnfvvynepf sqpppahmgi ppvhidpktg iprpsvyplt spgqythsmf
       61 sqefaqqlhw hsaaagmypm ppmasggfrg gssyapplpr lnppllppsi mphglhpalv
      121 tpgpkqelss plpdtrhpla myhpldsskc nqgrpssrgd lvngttagln ghgggppssq
      181 paghppsngs ssssqphqpq qqqhngtekk pqkptnhvkk plnafmlymk emrakvvaec
      241 tlkesaainq ilgrrwhsls reeqskyyem arkerqihmq lypgwtardn yainskkkkr
      301 kkdksqdgdl nnpkkcrary gldqqsdwck pcrrkkkcir yqegldgnan esednigsve
      361 aptpeshtsh gggdttttts tgspctslgt pepspgppvr rpdcyslnkh hplsvhhltg
      421 qplvkrepdd pvdiltppst dssttpsslp lltvt
//
LOCUS       XP_077506112             469 aa            linear   INV 25-NOV-2025
DEFINITION  coiled-coil domain-containing protein 6-like [Amblyomma
            americanum].
ACCESSION   XP_077506112
VERSION     XP_077506112.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649986.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..469
                     /product="coiled-coil domain-containing protein 6-like"
                     /calculated_mol_wt=52114
     Region          23..312
                     /region_name="DUF2046"
                     /note="Uncharacterized conserved protein H4 (DUF2046);
                     pfam09755"
                     /db_xref="CDD:401633"
     CDS             1..469
                     /gene="LOC144115561"
                     /coded_by="XM_077649986.1:77..1486"
                     /db_xref="GeneID:144115561"
ORIGIN      
        1 mflggsrnma dsasesdsss vdgamiapsp lsreqiqkrv eslqqenrvl kmeletyrlr
       61 vktlqeenra lrqvsvniqa kaeqeeefis ntllkkiqal kkeketlaln yeqeeecltn
      121 dlsrklnqlr qekvqleqsl eqeqealvnk lmrkierlea qtlakqanle qlrrekvele
      181 ctleqeqeal vnrlwkrmdk leaekrllqe rleqppsapp sprdpgldtp aglgahvarl
      241 rgelarlraq lhdtqqehne kvaqfaqeer qireenvrlq rklqleterr ealcrhlses
      301 esslemdeer hfnemsahgv rprtvsspip ynpspnssrp lspgvpvsgg pggipgslpr
      361 ealspsgsln rcqtcgqplf hpsgatvppp afllpspspp tvnmlpphpa sfpssnqarv
      421 rtssqerfsk sgvpssaaap aaspvapshl npvnpapatm papaspmmd
//
LOCUS       XP_077506113             328 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial import receptor subunit TOM40 homolog 1-like
            [Amblyomma americanum].
ACCESSION   XP_077506113
VERSION     XP_077506113.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649987.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..328
                     /product="mitochondrial import receptor subunit TOM40
                     homolog 1-like"
                     /calculated_mol_wt=34889
     Region          43..328
                     /region_name="Porin3_Tom40"
                     /note="Translocase of outer mitochondrial membrane 40
                     (Tom40); cd07305"
                     /db_xref="CDD:132766"
     CDS             1..328
                     /gene="LOC144115562"
                     /coded_by="XM_077649987.1:180..1166"
                     /db_xref="GeneID:144115562"
ORIGIN      
        1 mgnvfasspl spppvlapap ppsvpeppdq rglpaqddte tqnpgtledl hkkckdifpa
       61 nfeggrlmvn kglsnhfqis htlnmssltp sgyrfgatyv gtqqfgpaes ypvllgdidp
      121 sgnlnaniih qfnprirtki aaqvqdnryv atqltadyrg psytasatlg nidilnnsgv
      181 vvahylqnvv pnvalgaela fqygpqvpgg eiavlsmagr ytgpsyvvsg tlgmagahcc
      241 yyhrgsettq vgvevetnfr vgesvasigy qvdlpkanlv frgmvdsnwt vgavlekklq
      301 plpftfalsg mlnhaknqsr fgcgliig
//
LOCUS       XP_077506114             318 aa            linear   INV 25-NOV-2025
DEFINITION  receptor of activated protein kinase C 1 [Amblyomma americanum].
ACCESSION   XP_077506114
VERSION     XP_077506114.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649988.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..318
                     /product="receptor of activated protein kinase C 1"
                     /calculated_mol_wt=35811
     Region          7..311
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cd00200"
                     /db_xref="CDD:238121"
     Site            order(14,33,37,43..44,61..62,80,84,90..91,103..104,121,
                     126,132..133,146,165,170,176..177,190..191,209,213,
                     219..220,232,249,254,259..260,275..276,300,304,310..311)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          18..61
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          67..103
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          108..143
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          151..188
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          195..231
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          236..279
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          286..310
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..318
                     /gene="Rack1"
                     /coded_by="XM_077649988.1:117..1073"
                     /db_xref="GeneID:144115563"
ORIGIN      
        1 mseqlelkga lrghngwvtr iatnpkfpet vlsasrdktl ilweltkdet sygipkrrlk
       61 ghghfvtdvv mssdgqyals cswdktlrlw dlivgrttrr fedhhkdvls vafsadnrqi
      121 vsgsrdksik lwntlaqcky tienahndwv scvrfspnns npiivscgwd sevkvwnltn
      181 cklkkahded hkgylntvtv spdgslcasg gkdcqamlwd lneskrlytl dhqdiindlc
      241 fspnrywlcv atgaairiwd legkslvdel rpevmttnak aelpqcisma wsadgqtlfa
      301 gytdnlirvw qvsvgttr
//
LOCUS       XP_077506115             311 aa            linear   INV 25-NOV-2025
DEFINITION  protein phosphatase 2 catalytic subunit mts [Amblyomma americanum].
ACCESSION   XP_077506115
VERSION     XP_077506115.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649989.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..311
                     /product="protein phosphatase 2 catalytic subunit mts"
                     /calculated_mol_wt=35510
     Region          11..295
                     /region_name="MPP_PP2A_PP4_PP6"
                     /note="PP2A, PP4, and PP6 phosphoprotein phosphatases,
                     metallophosphatase domain; cd07415"
                     /db_xref="CDD:277360"
     Site            order(53,69,72..73,75..76,78,80..81,93,108..112,123..124,
                     127..128,132..133,136..137,145,270,279,289,295)
                     /site_type="other"
                     /note="heterotrimer interface [polypeptide binding]"
                     /db_xref="CDD:277360"
     Site            order(59,61,87,91,119..120,124..125,129,169,191..193,202,
                     215..217,243,245,269..271)
                     /site_type="active"
                     /db_xref="CDD:277360"
     Site            order(59,61,87,119,169,243)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:277360"
     CDS             1..311
                     /gene="mts"
                     /coded_by="XM_077649989.1:148..1083"
                     /db_xref="GeneID:144115564"
ORIGIN      
        1 msiedkaalk eldawieqln eckqltenqv ktlcdkakei lakesnvqev kcpvtvcgdv
       61 hgqfhdlmel friggkspdt nylfmgdyvd rgyysvetvs llvalkvryr eritilrgnh
      121 esrqitqvyg fydeclrkyg nanvwkyftd lfdylpltal vdgqifclhg glspsidtld
      181 hiraldrlqe vphegpmcdl lwsdpddrgg wgisprgagy tfgqdisetf nhsngltlva
      241 rahqlvmegy nwchdrnvvt ifsapnycyr cgnqaaimel ddqlkysflq fdpaprrgep
      301 hvtrrtpdyf l
//
LOCUS       XP_077506116             126 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115565 [Amblyomma americanum].
ACCESSION   XP_077506116
VERSION     XP_077506116.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649990.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..126
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..126
                     /product="uncharacterized protein LOC144115565"
                     /calculated_mol_wt=13782
     CDS             1..126
                     /gene="LOC144115565"
                     /coded_by="XM_077649990.1:147..527"
                     /db_xref="GeneID:144115565"
ORIGIN      
        1 mvfgpdvvss lalftlllkv gllllqlfan rgrpytaaet eeilgglvra lillmchnee
       61 erkryihlht lagtlceffg lvylldfita rqtesmsilq cavprmtsvr pakssvappt
      121 sptanh
//
LOCUS       XP_077506117             126 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115565 [Amblyomma americanum].
ACCESSION   XP_077506117
VERSION     XP_077506117.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649991.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..126
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..126
                     /product="uncharacterized protein LOC144115565"
                     /calculated_mol_wt=13782
     CDS             1..126
                     /gene="LOC144115565"
                     /coded_by="XM_077649991.1:147..527"
                     /db_xref="GeneID:144115565"
ORIGIN      
        1 mvfgpdvvss lalftlllkv gllllqlfan rgrpytaaet eeilgglvra lillmchnee
       61 erkryihlht lagtlceffg lvylldfita rqtesmsilq cavprmtsvr pakssvappt
      121 sptanh
//
LOCUS       XP_077506118             126 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115565 [Amblyomma americanum].
ACCESSION   XP_077506118
VERSION     XP_077506118.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649992.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..126
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..126
                     /product="uncharacterized protein LOC144115565"
                     /calculated_mol_wt=13782
     CDS             1..126
                     /gene="LOC144115565"
                     /coded_by="XM_077649992.1:147..527"
                     /db_xref="GeneID:144115565"
ORIGIN      
        1 mvfgpdvvss lalftlllkv gllllqlfan rgrpytaaet eeilgglvra lillmchnee
       61 erkryihlht lagtlceffg lvylldfita rqtesmsilq cavprmtsvr pakssvappt
      121 sptanh
//
LOCUS       XP_077506119             126 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115565 [Amblyomma americanum].
ACCESSION   XP_077506119
VERSION     XP_077506119.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649993.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..126
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..126
                     /product="uncharacterized protein LOC144115565"
                     /calculated_mol_wt=13782
     CDS             1..126
                     /gene="LOC144115565"
                     /coded_by="XM_077649993.1:147..527"
                     /db_xref="GeneID:144115565"
ORIGIN      
        1 mvfgpdvvss lalftlllkv gllllqlfan rgrpytaaet eeilgglvra lillmchnee
       61 erkryihlht lagtlceffg lvylldfita rqtesmsilq cavprmtsvr pakssvappt
      121 sptanh
//
LOCUS       XP_077506120             217 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115566 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506120
VERSION     XP_077506120.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649994.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="uncharacterized protein LOC144115566 isoform X1"
                     /calculated_mol_wt=24010
     Region          16..>55
                     /region_name="PRK03992"
                     /note="proteasome-activating nucleotidase; Provisional"
                     /db_xref="CDD:179699"
     Region          <40..63
                     /region_name="ZIP_TSC22D-like"
                     /note="leucine zipper found in the TSC22 domain leucine
                     zipper transcription factors, c-Myc-binding protein, and
                     similar proteins; cl40461"
                     /db_xref="CDD:424092"
     CDS             1..217
                     /gene="LOC144115566"
                     /coded_by="XM_077649994.1:4..657"
                     /db_xref="GeneID:144115566"
ORIGIN      
        1 mmitgfqvsq lsglsaylrq qvnslkaslk aatdnwikhk qenerlrkev aalrsevkel
       61 kdqqspgiei gqsptyltrp dylippafeq ptaappvarm edfgqrtpsm qnfsggaigy
      121 tpsrratamm astdlsqqrg hrtyahddqp asrldvaqat lafgssprer rslprsdssl
      181 ssirfrtprt dshsrfpvsr cfsedhtrsg kaspsrs
//
LOCUS       XP_077506121             172 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115566 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506121
VERSION     XP_077506121.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649995.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..172
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..172
                     /product="uncharacterized protein LOC144115566 isoform X2"
                     /calculated_mol_wt=18980
     Region          16..>55
                     /region_name="PRK03992"
                     /note="proteasome-activating nucleotidase; Provisional"
                     /db_xref="CDD:179699"
     CDS             1..172
                     /gene="LOC144115566"
                     /coded_by="XM_077649995.1:523..1041"
                     /db_xref="GeneID:144115566"
ORIGIN      
        1 mmitgfqvsq lsglsaylrq qvnslkaslk aatdnwikhk qenerlrkev aalrsevkel
       61 kdqqspgiei gqsptyltrp dylippafeq ptaappvarm edfgqrtpsm qnfsggaigy
      121 tpsrratamm astdlsqqrg hrtyahddqp asrldvaqat lafghyhapi rl
//
LOCUS       XP_077506122             415 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115568 [Amblyomma americanum].
ACCESSION   XP_077506122
VERSION     XP_077506122.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649996.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..415
                     /product="uncharacterized protein LOC144115568"
                     /calculated_mol_wt=45621
     Region          <226..>285
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     Region          309..398
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(325,327,329,354..356,392)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133151"
     Site            325..327
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133151"
     Site            325
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133151"
     Site            order(354..357,361..364)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133151"
     CDS             1..415
                     /gene="LOC144115568"
                     /coded_by="XM_077649996.1:79..1326"
                     /db_xref="GeneID:144115568"
ORIGIN      
        1 msisgleppp pflpvpgrpa vawpqwfrif enyvlasgas dctpdrrkal llhslgvegq
       61 rifytlplps ldnvklgeqt atektsedsk gtgdtrpevs sydiavaalh ahfsatsnvv
      121 aerhrfsrrv qqagesvney italwelsat csfpaeedsl rdqfvagiss rslrerllle
      181 gsslsfakav llarqfelay ndlqefpsvd vgrintrgnt ptrtpesdes skwshnrqyr
      241 cyrcgssrhn aasvrcpaks krchhcgvmg hfrsvcqkrg sapvrevdsq dspseevlsa
      301 vttaarsavy vtvsidradi tflvdsgssv sivahdlfkq yfegkvllap tvwlldyskq
      361 pipvrgcffa dvtykastts llfyvveqgt sllgidaika lglqighlcn alkrr
//
LOCUS       XP_077506123             332 aa            linear   INV 25-NOV-2025
DEFINITION  geranylgeranyl transferase type-2 subunit beta [Amblyomma
            americanum].
ACCESSION   XP_077506123
VERSION     XP_077506123.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649997.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..332
                     /product="geranylgeranyl transferase type-2 subunit beta"
                     /calculated_mol_wt=36916
     Region          18..301
                     /region_name="GGTase-II"
                     /note="Geranylgeranyltransferase type II (GGTase-II)_like
                     proteins containing the protein prenyltransferase (PTase)
                     domain, beta subunit (alpha 6 - alpha 6 barrel fold).
                     GGTase-IIs are a subgroup of the protein prenyltransferase
                     family of lipid-modifying...; cd02894"
                     /db_xref="CDD:239224"
     Site            order(41,44..45,48,52,94,96..97,99..100,144,147..148,190,
                     192,196,238,240..241,244,280,289..290)
                     /site_type="active"
                     /note="active site cavity [active]"
                     /db_xref="CDD:239224"
     Site            order(44..45,48,96..97,144,238,280)
                     /site_type="active"
                     /note="peptide binding pocket [active]"
                     /db_xref="CDD:239224"
     Site            order(48,51,144,147,190,192,196,232,235,241,244,289)
                     /site_type="lipid-binding"
                     /note="lipid binding pocket [chemical binding]"
                     /db_xref="CDD:239224"
     Site            order(139,177,187..188,193,217,220,222..223,232..234)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:239224"
     Site            order(238,240,290)
                     /site_type="other"
                     /note="Zn2+ binding site [ion binding]"
                     /db_xref="CDD:239224"
     CDS             1..332
                     /gene="RabGGTb"
                     /coded_by="XM_077649997.1:388..1386"
                     /db_xref="GeneID:144115570"
ORIGIN      
        1 mamlvkdvhl psspsqelnl akhkeylasy gtkkddyeyc itehlrmsgi ywgltamdlm
       61 gsldsfdnae iiefvkqcqy scggfgasih hdphllytls avqilatfda lntididktv
      121 syvkelqqed gsfygdkwge vdtrfsfcav aclallnklh ainiekavef vvscmnfdgg
      181 fgcrpgseth sgqiycclgt lsilgrlhhi nadllgwwlc erqlpsggln grpeklpdvc
      241 yswwvlaslk iigrlhwidk eklqnfilas qdeetggfgd rpgdmvdpfh tlfglaglsl
      301 lgderlkpvn pvfcmseevv arlgvrhqll sl
//
LOCUS       XP_077506124             668 aa            linear   INV 25-NOV-2025
DEFINITION  sec1 family domain-containing protein 2-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506124
VERSION     XP_077506124.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649998.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..668
                     /product="sec1 family domain-containing protein 2-like
                     isoform X1"
                     /calculated_mol_wt=72745
     Region          <563..658
                     /region_name="Sec1"
                     /note="Sec1 family; cl15415"
                     /db_xref="CDD:472804"
     CDS             1..668
                     /gene="LOC144115573"
                     /coded_by="XM_077649998.1:588..2594"
                     /db_xref="GeneID:144115573"
ORIGIN      
        1 mhqnvfrspl egpvkrlwee vfprcqdavv fldnamaeal hrnggasklf esgavavref
       61 sffehgstse pkalflvstp ltqqtlstls aivrasvftn vnvltscppa aqilakygta
      121 easdspqafl dveerlldwm gnmtfvaevm yvplsvinvt pkfflmpqfh dvfpplqkqy
      181 ntsqvgatle gasftrsmqi nvkslignln sifevahike etfslgslsr ivgeelsrls
      241 rghrreqpvl agdaniirnv sakskvslll ldctldmags lsascesfld tlvrvlppfp
      301 ghrldvcidt rcissspepa vpsdavcpgc lghedsklal qhiinykqke cmmelnrllv
      361 esalkqgvrl ditgrltaeq lrkrvlqfrk dggdaiidkg liqqilgvaq avlegryskl
      421 edllstekvl vqnasvssea asrslvqllh ssnttglqle diivllavlc slyggrtlgn
      481 esdrrtlkae ivaaiyqaag egslpacleh fvsdeletld estviqkvdg ifralqslgm
      541 araqlrkyrs iieednvvqp avykpllkqv lqdifdpsas avpdldhhsg glsdifkagf
      601 glfrsatkps pkdspvliif iiggvtasev klvnditssr kltqqvitgs ttlakpsqql
      661 gllfehct
//
LOCUS       XP_077506125             653 aa            linear   INV 25-NOV-2025
DEFINITION  sec1 family domain-containing protein 2-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506125
VERSION     XP_077506125.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077649999.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..653
                     /product="sec1 family domain-containing protein 2-like
                     isoform X2"
                     /calculated_mol_wt=71154
     Region          <548..643
                     /region_name="Sec1"
                     /note="Sec1 family; cl15415"
                     /db_xref="CDD:472804"
     CDS             1..653
                     /gene="LOC144115573"
                     /coded_by="XM_077649999.1:588..2549"
                     /db_xref="GeneID:144115573"
ORIGIN      
        1 mhqnvfrspl egpvkrlwee vfprcqdavv fldnamaeal hrnggasklf esgavavref
       61 sffehgstse pkalflvstp ltqqtlstls aivrasvftn vnvltscppa aqilakygta
      121 easdspqafl dveerlldwm gnmtfvaevm yvplsvinvt pkfflmpqfh dvfpplqkqy
      181 ntsqvgatle gasftrsmqi nvkslignln sifevahike etfslgslsr ivgeelsrls
      241 rghrrsaksk vslllldctl dmagslsasc esfldtlvrv lppfpghrld vcidtrciss
      301 spepavpsda vcpgclghed sklalqhiin ykqkecmmel nrllvesalk qgvrlditgr
      361 ltaeqlrkrv lqfrkdggda iidkgliqqi lgvaqavleg ryskledlls tekvlvqnas
      421 vsseaasrsl vqllhssntt glqlediivl lavlcslygg rtlgnesdrr tlkaeivaai
      481 yqaagegslp aclehfvsde letldestvi qkvdgifral qslgmaraql rkyrsiieed
      541 nvvqpavykp llkqvlqdif dpsasavpdl dhhsgglsdi fkagfglfrs atkpspkdsp
      601 vliifiiggv tasevklvnd itssrkltqq vitgsttlak psqqlgllfe hct
//
LOCUS       XP_077506126            1016 aa            linear   INV 25-NOV-2025
DEFINITION  protein argonaute-2-like [Amblyomma americanum].
ACCESSION   XP_077506126
VERSION     XP_077506126.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650000.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1016
                     /product="protein argonaute-2-like"
                     /calculated_mol_wt=112809
     Region          <5..>86
                     /region_name="PRK07764"
                     /note="DNA polymerase III subunits gamma and tau;
                     Validated"
                     /db_xref="CDD:236090"
     Region          155..1015
                     /region_name="PLN03202"
                     /note="protein argonaute; Provisional"
                     /db_xref="CDD:215631"
     Region          543..972
                     /region_name="Piwi_ago-like"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; cd04657"
                     /db_xref="CDD:240015"
     Site            order(675,679,691..694,697,706,709,713,717)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     Site            order(744,746,822,962)
                     /site_type="active"
                     /db_xref="CDD:240015"
     CDS             1..1016
                     /gene="LOC144115575"
                     /coded_by="XM_077650000.1:28..3078"
                     /db_xref="GeneID:144115575"
ORIGIN      
        1 mgkgerkdkk kndsaggppa vpsasggnqa tapapspagt srdlfaqvlq qgaapspqpr
       61 avsrgpapaa vpraqptaqp taqpsayrps ygaaaaagda nvaassprpa saaaavsrsq
      121 paaaalpppp pctppvavte qtpeagdqat pggdvsikel ertlpshfpr rpahgqlgrt
      181 iqllanhfsi eiptgsvyhy dvdissetak etkvpeqkny rclstkinri viellvkkyr
      241 qdlancipvf dghknlytrr qlnfrertft vdldedqrsq kfiikiqyat tvnldalhgv
      301 fqkrvqtvpq evlqaidivl rhspsinlap vgrsffrppg pnehnhlggg revwfgyyas
      361 vrpaqwkpml nvdmsatafy eslplvdfmc rffsdsrrvl tpadlrslrd nqyarlnrel
      421 kglrvkvthl pyprkykvvk itreaakdiy fesegsqisv adyfqsryrr lsypnfpcvq
      481 sgspthpvyi plevcelaeg qhcrkklees qtaemikrta kppakrflei rqsvrdmvst
      541 sdkylrefgv kinpeptqvv grildppslv fennsmckpr dgtwdlrgqr fykamsmtqw
      601 ivlnvsrfaq kdcldnfikm lvrigqglgm riaqplavit fdtnrkpmrt vlteqrkqhp
      661 qlemvvavit katnyaeikq vaetelslrt qcildnnvvq kcnaaliqnl cqkinakmgg
      721 innslllqek pkllhrpviv igadvshpsp gdkirpsiaa cvgsldsvps kfhatirvqi
      781 edskakarve iikdlkdmik elllvfyrtt ghkplgivfy rdgvsegqfl evrnhevsai
      841 rlacaemcpt etyeppltfi vvqkrhhtrf mpandrdgvg kfrnvppgtt vdsvvthpld
      901 fdfflcshfg iqgtsrpahy yivwddsnfs addlqklsfy lchtysrcar svsipapvyy
      961 ahlaafrakh hiaskletsg avsqsseggg davlttaqyv eavkvlqelq asmyfv
//
LOCUS       XP_077506127             248 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase RNF212B-like [Amblyomma americanum].
ACCESSION   XP_077506127
VERSION     XP_077506127.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650001.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..248
                     /product="E3 ubiquitin-protein ligase RNF212B-like"
                     /calculated_mol_wt=27480
     Region          5..45
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(7,10,25,27,30,33,42,45)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          <89..163
                     /region_name="FtsB"
                     /note="Cell division protein FtsB [Cell cycle control,
                     cell division, chromosome partitioning]; COG2919"
                     /db_xref="CDD:442163"
     Region          <116..139
                     /region_name="ZIP_TSC22D-like"
                     /note="leucine zipper found in the TSC22 domain leucine
                     zipper transcription factors, c-Myc-binding protein, and
                     similar proteins; cl40461"
                     /db_xref="CDD:424092"
     CDS             1..248
                     /gene="LOC144115576"
                     /coded_by="XM_077650001.1:325..1071"
                     /db_xref="GeneID:144115576"
ORIGIN      
        1 mnklvycnnc faqptgtslf fvtncahvfc rgckeectrs kckicnatcs taplsqnmsa
       61 dvrelfkdpk iimqrammit gfqvsqlsgl saylrqqvns lkaslkaatd nwikhkqene
      121 rlrkevaalr sevkelkdqq spgieiaqsp tfltrpdyli ppafeqptaa ppvarmedfg
      181 qrtpsmqnfs ggaigytpsr ratammastd lsqqrghrty ahddqpasrl dvaqatlafg
      241 hyhapirl
//
LOCUS       XP_077506128             248 aa            linear   INV 25-NOV-2025
DEFINITION  E3 ubiquitin-protein ligase RNF212B-like [Amblyomma americanum].
ACCESSION   XP_077506128
VERSION     XP_077506128.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650002.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..248
                     /product="E3 ubiquitin-protein ligase RNF212B-like"
                     /calculated_mol_wt=27480
     Region          5..45
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(7,10,25,27,30,33,42,45)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          <89..163
                     /region_name="FtsB"
                     /note="Cell division protein FtsB [Cell cycle control,
                     cell division, chromosome partitioning]; COG2919"
                     /db_xref="CDD:442163"
     Region          <116..139
                     /region_name="ZIP_TSC22D-like"
                     /note="leucine zipper found in the TSC22 domain leucine
                     zipper transcription factors, c-Myc-binding protein, and
                     similar proteins; cl40461"
                     /db_xref="CDD:424092"
     CDS             1..248
                     /gene="LOC144115576"
                     /coded_by="XM_077650002.1:352..1098"
                     /db_xref="GeneID:144115576"
ORIGIN      
        1 mnklvycnnc faqptgtslf fvtncahvfc rgckeectrs kckicnatcs taplsqnmsa
       61 dvrelfkdpk iimqrammit gfqvsqlsgl saylrqqvns lkaslkaatd nwikhkqene
      121 rlrkevaalr sevkelkdqq spgieiaqsp tfltrpdyli ppafeqptaa ppvarmedfg
      181 qrtpsmqnfs ggaigytpsr ratammastd lsqqrghrty ahddqpasrl dvaqatlafg
      241 hyhapirl
//
LOCUS       XP_077506129             345 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115578 [Amblyomma americanum].
ACCESSION   XP_077506129
VERSION     XP_077506129.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650003.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 14% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..345
                     /product="uncharacterized protein LOC144115578"
                     /calculated_mol_wt=39261
     Region          <43..>182
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..345
                     /gene="LOC144115578"
                     /coded_by="XM_077650003.1:1..1038"
                     /db_xref="GeneID:144115578"
ORIGIN      
        1 mrppnplcff vqvssvisly akksndvcll lfprpgvllc vfsecfdivn qllalsgdve
       61 lnpghntrhq vqdslpdvqs kqledmfail etlntrtakm qldqksfiks iddlkksqlk
      121 ledlsemnkr ltnverktvt idetekevhp fqqlvdqlss ensqlrarls eledrqrrdn
      181 llfygipdaq svswaqseek lvkvlsscle ipsseilidr ahrlggftse kcrpiiakfs
      241 sfkvkqrill ncsklkdeki tlsedfsfat rharkklvef gksqpltfkl rfnklyiknk
      301 cysynhsdds vheipsafpl ptdrsedptn dthsasappt elvtn
//
LOCUS       XP_077506130             439 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115580 [Amblyomma americanum].
ACCESSION   XP_077506130
VERSION     XP_077506130.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650004.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..439
                     /product="uncharacterized protein LOC144115580"
                     /calculated_mol_wt=50370
     Region          5..104
                     /region_name="SNARE_NTD_STX6_STX10"
                     /note="N-terminal domain of syntaxin-6, syntaxin-10, and
                     similar proteins; cd21443"
                     /db_xref="CDD:410569"
     Region          235..319
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     Region          390..424
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..439
                     /gene="LOC144115580"
                     /coded_by="XM_077650004.1:212..1531"
                     /db_xref="GeneID:144115580"
ORIGIN      
        1 msledpffvv kdeivkaian nktlferwnq yqdpsslpsk edidwttsel rkglrsiewd
       61 ledleetvai veknpkkfki dekeiksrka fieqsrnevk cmkeailesk aknkrmrpss
      121 melfnssrta kytslrneve spvhrlldht qqqqqqrtsq rkksawrrce rmlavpvhvr
      181 fprqlmvalw waessvshrr kdeedagtrl rspiptttis apfhrvrama sfneklvqev
      241 kkhtqlwdqq sklhkeagyr eaawmdiaaa lavtveecqt lwrtlrdtyl krkkrrraga
      301 ngqwnvlerd lgflddflrp rtrrstlnaa apadvevkqe qpgedaetre aavaaaaapp
      361 qpwhlamrpa avmpdrlaag gaaaqelssd peeffclsla ahlkrlvpre rsiarmkmle
      421 tlhnlefgdn igeaalgep
//
LOCUS       XP_077506131             211 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115582 [Amblyomma americanum].
ACCESSION   XP_077506131
VERSION     XP_077506131.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650005.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..211
                     /product="uncharacterized protein LOC144115582"
                     /calculated_mol_wt=23531
     Region          7..91
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     Region          162..196
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..211
                     /gene="LOC144115582"
                     /coded_by="XM_077650005.1:57..692"
                     /db_xref="GeneID:144115582"
ORIGIN      
        1 masfneklvq evkkhtqlwd qhsrlhkkag yreaawmeia aalavtveec qtrwrtlrdt
       61 ylkrkkrrra gangqwnvle rdlgflddfl rprtrrstln aaapadvevk qeqpgedaet
      121 reaavaaaaa ppqpwplamr paavmpdrla aggaaaqels sdpeeffcls laahlkrlvp
      181 rersiarmkm letlhnlefg dnigeaalge p
//
LOCUS       XP_077506132             262 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115583 [Amblyomma americanum].
ACCESSION   XP_077506132
VERSION     XP_077506132.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650006.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..262
                     /product="uncharacterized protein LOC144115583"
                     /calculated_mol_wt=27209
     CDS             1..262
                     /gene="LOC144115583"
                     /coded_by="XM_077650006.1:888..1676"
                     /db_xref="GeneID:144115583"
ORIGIN      
        1 mvrlsrrhlq rdipkkeatr gttrsitshr vvitrkmqss hpaqtllsqa patvslangs
       61 rpgrklpsgs vqrrgqraaa saathtacfw ytrstrklnr alpllarhtt pcsaaaapvg
      121 ttdgrdvaap gsnwvasraa dlrrrscaps pgrvlgasrr rpvgrtpsca saeesysags
      181 atrgagtqph srpgigvatg pgtssgvgqs arvrairlpt patvrdllvv psssgpatpt
      241 lgsakappel hlnfqlqsfg ss
//
LOCUS       XP_077506133             424 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115584 [Amblyomma americanum].
ACCESSION   XP_077506133
VERSION     XP_077506133.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650007.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..424
                     /product="uncharacterized protein LOC144115584"
                     /calculated_mol_wt=48899
     Region          5..104
                     /region_name="SNARE_NTD_STX6_STX10"
                     /note="N-terminal domain of syntaxin-6, syntaxin-10, and
                     similar proteins; cd21443"
                     /db_xref="CDD:410569"
     Region          235..319
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     Region          390..424
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..424
                     /gene="LOC144115584"
                     /coded_by="XM_077650007.1:197..1471"
                     /db_xref="GeneID:144115584"
ORIGIN      
        1 msledpffvv kdeivkaian nktlferwnq yqdpsslpsk edidwttsel rkglrsiewd
       61 ledleetvai veknpkkfki dekeiksrka fieqsrnevk cmkeailesk aknkrmrpss
      121 melfnssrta kytslrneve spvhrlldht qqqqqqrtsq rkksawrrce rmlavpvhvr
      181 fprqlvvalw waersvshrr kdeedggtrl rspiptttis apfhrvrama sfneklvqev
      241 nkhtqlwdqq sklhkeagyr eaawmeiaaa ldvtveecqt rwrtlrdtyl krkkrrraga
      301 ngqwsvlerd lgflddflrp rtrrstlnaa apadvevkqe qpgedaetre aavaaaaapp
      361 qpwplamrpa avmpdrlaag gaaaqelssd peeffclsla ahlkrlvpre rsiarmkmle
      421 tlhn
//
LOCUS       XP_077506134             439 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115586 [Amblyomma americanum].
ACCESSION   XP_077506134
VERSION     XP_077506134.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650008.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..439
                     /product="uncharacterized protein LOC144115586"
                     /calculated_mol_wt=50370
     Region          5..104
                     /region_name="SNARE_NTD_STX6_STX10"
                     /note="N-terminal domain of syntaxin-6, syntaxin-10, and
                     similar proteins; cd21443"
                     /db_xref="CDD:410569"
     Region          235..319
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     Region          390..424
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..439
                     /gene="LOC144115586"
                     /coded_by="XM_077650008.1:212..1531"
                     /db_xref="GeneID:144115586"
ORIGIN      
        1 msledpffvv kdeivkaian nktlferwnq yqdpsslpsk edidwttsel rkglrsiewd
       61 ledleetvai veknpkkfki dekeiksrka fieqsrnevk cmkeailesk aknkrmrpss
      121 melfnssrta kytslrneve spvhrlldht qqqqqqrtsq rkksawrrce rmlavpvhvr
      181 fprqlmvalw waessvshrr kdeedagtrl rspiptttis apfhrvrama sfneklvqev
      241 kkhtqlwdqq sklhkeagyr eaawmdiaaa lavtveecqt lwrtlrdtyl krkkrrraga
      301 ngqwnvlerd lgflddflrp rtrrstlnaa apadvevkqe qpgedaetre aavaaaaapp
      361 qpwhlamrpa avmpdrlaag gaaaqelssd peeffclsla ahlkrlvpre rsiarmkmle
      421 tlhnlefgdn igeaalgep
//
LOCUS       XP_077506135             211 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115587 [Amblyomma americanum].
ACCESSION   XP_077506135
VERSION     XP_077506135.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650009.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..211
                     /product="uncharacterized protein LOC144115587"
                     /calculated_mol_wt=23504
     Region          7..91
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     Region          162..196
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..211
                     /gene="LOC144115587"
                     /coded_by="XM_077650009.1:128..763"
                     /db_xref="GeneID:144115587"
ORIGIN      
        1 masfneklvq evkkhtqlwd qhsrlhkkag yreaawmeia aalavtveec qtrwrtlrdt
       61 ylkrkkrrra gangqwsvle rdlgflddfl rprtrrstln aaapadvevk qeqpgedaet
      121 reaavaaaaa ppqpwplamr paavmpdrla aggaaaqels sdpeeffcls laahlkrlvp
      181 rersiarmkm letlhnlefg dnigeaalge p
//
LOCUS       XP_077506136             435 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115589 [Amblyomma americanum].
ACCESSION   XP_077506136
VERSION     XP_077506136.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650010.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..435
                     /product="uncharacterized protein LOC144115589"
                     /calculated_mol_wt=49869
     Region          5..104
                     /region_name="SNARE_NTD_STX6_STX10"
                     /note="N-terminal domain of syntaxin-6, syntaxin-10, and
                     similar proteins; cd21443"
                     /db_xref="CDD:410569"
     Region          235..319
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     Region          386..420
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..435
                     /gene="LOC144115589"
                     /coded_by="XM_077650010.1:215..1522"
                     /db_xref="GeneID:144115589"
ORIGIN      
        1 msledpffvv kdeivkaian nktvferwnq yqdpsslpsk edidwttsel rkglrsiewd
       61 ledleetvai veknpkkfki dekeiksrka fieqsrnevk cmkeailesk aknkrmrpss
      121 melfnssrta kytslrneve spvhrlldht qqqqqqrtsq rkksawrrce rmlavpvhvr
      181 fprqlvvalw waersvshrr kdeedggtrl rspiptttis apfhrvrama sfneklvqev
      241 kkhtqlwdqq sklhkeagyr eaawmeiaaa ldvtveecqt rwrtlrdtyl krkkrrraga
      301 ngqwnvlerd lgflddflrp rtrrstlnaa apadvevkqe qpgedaemre aavaaaaapp
      361 lamrpaavmp hrlaaggaaa qelssdpeei fclclaahlr glpprdrsia mmkmlqtlhd
      421 lesgenigef algdp
//
LOCUS       XP_077506137             495 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115591 [Amblyomma americanum].
ACCESSION   XP_077506137
VERSION     XP_077506137.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650011.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..495
                     /product="uncharacterized protein LOC144115591"
                     /calculated_mol_wt=56053
     CDS             1..495
                     /gene="LOC144115591"
                     /coded_by="XM_077650011.1:89..1576"
                     /db_xref="GeneID:144115591"
ORIGIN      
        1 mlplklwsgd htvkkignas tlsevvkqaq ekgvctsena kvflrdwtev eedffqevie
       61 hllleerifi vtdtaptter tlqghsgtqn msssnpeget aalqvdftfk vdtgsfpetv
      121 laclrpggpl lrpkdrrdlv qcivgqmvak csrprrefir svaekvveef pilkdrgvdg
      181 hllgrgydsl fqqmenrven mtrgkrqpsq vpdrkskkra nvsygclnwq pilnssgdaa
      241 qekvrflrqe glksareinl plalqymqet ygdqrdyins nnpvhqtrei kaqwpllfha
      301 rffyghadql lgkdvkaise ektalcasal anflrdsnkk evlyyyvqme leesrgnrga
      361 keaaflplla alfkddvgql frvfeegtsi terlhelpct ptvvalgsff qnqcyiiceq
      421 qvlfseptgf geatrlafla yyvfnmsypv kaattleflq reifdinptk gtkwngkqkc
      481 rtivhakimk laanl
//
LOCUS       XP_077506138             439 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115593 [Amblyomma americanum].
ACCESSION   XP_077506138
VERSION     XP_077506138.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650012.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..439
                     /product="uncharacterized protein LOC144115593"
                     /calculated_mol_wt=50439
     Region          5..104
                     /region_name="SNARE_NTD_STX6_STX10"
                     /note="N-terminal domain of syntaxin-6, syntaxin-10, and
                     similar proteins; cd21443"
                     /db_xref="CDD:410569"
     Region          235..319
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     Region          390..424
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..439
                     /gene="LOC144115593"
                     /coded_by="XM_077650012.1:211..1530"
                     /db_xref="GeneID:144115593"
ORIGIN      
        1 msledpffvv kdeivkaian nktlferwnq yqdpsslpsk edidwttsel rkglrsiewd
       61 ledleetvai veknpkkfki dekeiksrka fieqsrnevk cmkeailesk aknkrmrpss
      121 melfnssrta kytslrneve spvhrlldht qqqqqqrtsq rkksawrrce rmlavpvhvr
      181 fprqlvvalw waersvshrr kdeedggtrl rspiptttis apfhrvrama sfneklvqev
      241 nkhtqlwdqq sklhkeagyr eaawmeiaaa ldvtveecqt rwrtlrdtyl krkkrrraga
      301 ngqwsvlerd lgflddflrp rtrrstlnaa apadvevkqe qpgedaetre aavaaaaapp
      361 qpwplamrpa avmpdrlaag gaaaqelssd peeffclsla ahlkrlvpre rsiarmkmle
      421 tlynlefgdn igeaalgep
//
LOCUS       XP_077506139             247 aa            linear   INV 25-NOV-2025
DEFINITION  syntaxin-6-like [Amblyomma americanum].
ACCESSION   XP_077506139
VERSION     XP_077506139.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650013.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..247
                     /product="syntaxin-6-like"
                     /calculated_mol_wt=28264
     Region          5..104
                     /region_name="SNARE_NTD_STX6_STX10"
                     /note="N-terminal domain of syntaxin-6, syntaxin-10, and
                     similar proteins; cd21443"
                     /db_xref="CDD:410569"
     Region          158..223
                     /region_name="SNARE_Syntaxin6"
                     /note="SNARE motif of syntaxin 6; cd15851"
                     /db_xref="CDD:277204"
     Site            order(162..165,167..169,171..173,175..176,178..183,
                     185..186,188..189,192..193,195..197,199..201,203..204,
                     206..207,210..211,213..214,216)
                     /site_type="other"
                     /note="heterotetramer interface [polypeptide binding]"
                     /db_xref="CDD:277204"
     Site            189
                     /site_type="other"
                     /note="zero layer"
                     /db_xref="CDD:277204"
     CDS             1..247
                     /gene="LOC144115594"
                     /coded_by="XM_077650013.1:200..943"
                     /db_xref="GeneID:144115594"
ORIGIN      
        1 msledpffvv kdeivkaian nktlferwnq yqdpsslpsk edidwttsel rkglrsiewd
       61 ledleetvai veknpkkfki dekeiksrka fieqsrnevk cmkeailesk aknkrmrpss
      121 melfnssrta kytslrneve spvhrlldht qqqqqelmva qddelegiqt svgtlksmsk
      181 qigneldeqs vmlddlghdm dnaeskidga lkkmakvlhm sndrrqwmai galsgvmvvv
      241 valffll
//
LOCUS       XP_077506140             632 aa            linear   INV 25-NOV-2025
DEFINITION  sodium-dependent multivitamin transporter-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077506140
VERSION     XP_077506140.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650014.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..632
                     /product="sodium-dependent multivitamin transporter-like
                     isoform X1"
                     /calculated_mol_wt=68842
     Region          40..558
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     Site            order(90,93,376,379..380)
                     /site_type="other"
                     /note="Na binding site [ion binding]"
                     /db_xref="CDD:271383"
     CDS             1..632
                     /gene="LOC144115596"
                     /coded_by="XM_077650014.1:272..2170"
                     /db_xref="GeneID:144115596"
ORIGIN      
        1 mnvaslcvfe nrahtsrgql sashraagvg sgmaaahvld yvlygllmgl gvaqglyvwc
       61 rrrrghderh etflggqslg vlplavsvlv stakplglva ltahfyaygm hvawsvlltv
      121 samplvvyfv vpvfyrlgvt sifeyirmry nafisltaca iylilmqnvg aalifsaalt
      181 sstifgipvt aasimfglvg tyyaavaglr gvvwtdclqa litmlaplvi igkvaydstv
      241 gslplrpwdd lqvdkyildv sfdltkeenv wsclvggaas flyrsgfdqm vlqrylaskn
      301 lnaaqrtaiv galfsalyyl ilmaaagami yrfrdcdpll sksisnidql lpfyvkrela
      361 pllgfsgifl agvvssstsa assivnsqaa vlyvdvvaqy fpitekwasr ttkalafasg
      421 iimtayslav pflgstmrif llinsavtgp fvgllvlail fpfanskgag aaacltiafq
      481 vwhmigrlhs gilpdrmpvt lehcpdntta lavmqnltnv edaeryedvf mlyrlssfws
      541 sffsviatil igivislltg grksasmheh lvddklrsaw rkttrrdgsh keeqtprete
      601 stsrtphvpa vdeptfiidr eelerltket qv
//
LOCUS       XP_077506141             523 aa            linear   INV 25-NOV-2025
DEFINITION  sodium-coupled monocarboxylate transporter 2-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077506141
VERSION     XP_077506141.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650015.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..523
                     /product="sodium-coupled monocarboxylate transporter
                     2-like isoform X2"
                     /calculated_mol_wt=57278
     Region          27..449
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     CDS             1..523
                     /gene="LOC144115596"
                     /coded_by="XM_077650015.1:486..2057"
                     /db_xref="GeneID:144115596"
ORIGIN      
        1 mhvawsvllt vsamplvvyf vvpvfyrlgv tsifeyirmr ynafisltac aiylilmqnv
       61 gaalifsaal tsstifgipv taasimfglv gtyyaavagl rgvvwtdclq alitmlaplv
      121 iigkvaydst vgslplrpwd dlqvdkyild vsfdltkeen vwsclvggaa sflyrsgfdq
      181 mvlqrylask nlnaaqrtai vgalfsalyy lilmaaagam iyrfrdcdpl lsksisnidq
      241 llpfyvkrel apllgfsgif lagvvsssts aassivnsqa avlyvdvvaq yfpitekwas
      301 rttkalafas giimtaysla vpflgstmri fllinsavtg pfvgllvlai lfpfanskga
      361 gaaacltiaf qvwhmigrlh sgilpdrmpv tlehcpdntt alavmqnltn vedaeryedv
      421 fmlyrlssfw ssffsviati ligivisllt ggrksasmhe hlvddklrsa wrkttrrdgs
      481 hkeeqtpret estsrtphvp avdeptfiid reelerltke tqv
//
LOCUS       XP_077506142             217 aa            linear   INV 25-NOV-2025
DEFINITION  zinc finger CCHC domain-containing protein 24-like [Amblyomma
            americanum].
ACCESSION   XP_077506142
VERSION     XP_077506142.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650016.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..217
                     /product="zinc finger CCHC domain-containing protein
                     24-like"
                     /calculated_mol_wt=24172
     Region          105..125
                     /region_name="zf-CCHC_2"
                     /note="Zinc knuckle; pfam13696"
                     /db_xref="CDD:463959"
     Region          140..206
                     /region_name="zf-3CxxC_2"
                     /note="Zinc-binding domain; pfam17180"
                     /db_xref="CDD:407306"
     CDS             1..217
                     /gene="LOC144115597"
                     /coded_by="XM_077650016.1:264..917"
                     /db_xref="GeneID:144115597"
ORIGIN      
        1 mtavessaav fqqpsppgql lnlvyltlad qtlhavdqik ygmtpvvaps syrgesycna
       61 yatgypngys egycteytga pfpdladqfs elslgadpra arkpppnylc hlcfckghyi
      121 kdcpqarpkg egltpyqgkk rcfgeykcpk ckrkwmsgns wanmgqkcik chlnvyphkq
      181 rplekpdgld vsdqskehpq hlcekckslg yycrrvv
//
LOCUS       XP_077506143             580 aa            linear   INV 25-NOV-2025
DEFINITION  sodium-coupled monocarboxylate transporter 1-like [Amblyomma
            americanum].
ACCESSION   XP_077506143
VERSION     XP_077506143.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650017.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..580
                     /product="sodium-coupled monocarboxylate transporter
                     1-like"
                     /calculated_mol_wt=63121
     Region          8..522
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     Site            order(57,60,343,346..347)
                     /site_type="other"
                     /note="Na binding site [ion binding]"
                     /db_xref="CDD:271383"
     CDS             1..580
                     /gene="LOC144115598"
                     /coded_by="XM_077650017.1:185..1927"
                     /db_xref="GeneID:144115598"
ORIGIN      
        1 metawaeytv favlmganfg lglyfsfyrr nrkstadevf lgsrslhalp lavsalasim
       61 ssigivgvsa hvyaygthmf wnqvlaplna livahivvpv lyrlrvtsvf eylrmrygdk
      121 vgltscvlyf flsqtmgava ifpaaaavat ifglsanwcs ftiglagciy talgglrgvv
      181 wtdcaqagiv llapatimvk iaydahakdl nlrpfsdfpv ktfmwensld ftkdenvwac
      241 liglstmyvy rggadqmivq rflasrnlke aqrtakvgtm lvliyygias slgvaliywf
      301 rdcdpllsga iksydqivpy yvkknlkefp cfsglflagv vsaatstiss linsqaaviy
      361 vdvvarffnl dekhsatvtk gialvvggvm tayaiaaphl gsaakiimvf ynsatgpflg
      421 lffmalvfpw angigasiat tlfsclviwq avgktfsgvr fpkmpvtter cplnttltsa
      481 ytseessvnf ddlfplykfs sywsgflsas fticlglais lltggrkqyq qnlpmtssff
      541 islwsklgll kmsdpmcqqt ssflentach vaqeepstav
//
LOCUS       XP_077506144             665 aa            linear   INV 25-NOV-2025
DEFINITION  sodium-coupled monocarboxylate transporter 2-like isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077506144
VERSION     XP_077506144.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650018.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..665
                     /product="sodium-coupled monocarboxylate transporter
                     2-like isoform X1"
                     /calculated_mol_wt=72961
     Region          71..599
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     Site            order(123,126,409,412..413)
                     /site_type="other"
                     /note="Na binding site [ion binding]"
                     /db_xref="CDD:271383"
     CDS             1..665
                     /gene="LOC144115599"
                     /coded_by="XM_077650018.1:61..2058"
                     /db_xref="GeneID:144115599"
ORIGIN      
        1 mpaaglrsqi tfgmslpelv eqesthadtl slrsyynaer rggswqrarh yarsapkrkr
       61 rvarsamhse veyavfgalm canlgfglyf scvrrarkea ttdemflgnr slrtlplags
      121 vlasmlsaig vigfsahyya ygfhylwnfl sapliavivs kvvipvlyql kvtsvfeylr
      181 mrygnkigla acsiyffisv tigavalfta svavstifnv slfwstvgig iastiytslg
      241 gmrgvvwtdc vqsllilmap vtvvtkviyd shhrdaslrp asdfnikdyi fdasldftkd
      301 envwacligl fihslfrsgm dqvvvqryla arklkeaqsi tyigitlnsm ynfimagmav
      361 alvywyrdcd pllsgaitkl dqmlpyyvke nlsdfpgfsg lfltgvvsaa tstvssiins
      421 qaavfyvdvi sqymkfttqq aasftkksal ffgcvltaca vavpylgsav rlilvlhsgg
      481 tapfvgifil glafpwanak gaavgtlvag glqlwlmfgk ialdihsprm pvtldycpvn
      541 vsalvanssa sgfdkassle rvsgfeensl lsfyrfsayw slifttalti iigvtvsllt
      601 gekntcagnf hltsklalml wrklnllpkp evklpaekge eedeknilcn efkreesfns
      661 neinv
//
LOCUS       XP_077506145             599 aa            linear   INV 25-NOV-2025
DEFINITION  sodium-coupled monocarboxylate transporter 2-like isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077506145
VERSION     XP_077506145.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650019.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..599
                     /product="sodium-coupled monocarboxylate transporter
                     2-like isoform X2"
                     /calculated_mol_wt=65461
     Region          5..533
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     Site            order(57,60,343,346..347)
                     /site_type="other"
                     /note="Na binding site [ion binding]"
                     /db_xref="CDD:271383"
     CDS             1..599
                     /gene="LOC144115599"
                     /coded_by="XM_077650019.1:159..1958"
                     /db_xref="GeneID:144115599"
ORIGIN      
        1 mhseveyavf galmcanlgf glyfscvrra rkeattdemf lgnrslrtlp lagsvlasml
       61 saigvigfsa hyyaygfhyl wnflsaplia vivskvvipv lyqlkvtsvf eylrmrygnk
      121 iglaacsiyf fisvtigava lftasvavst ifnvslfwst vgigiastiy tslggmrgvv
      181 wtdcvqslli lmapvtvvtk viydshhrda slrpasdfni kdyifdasld ftkdenvwac
      241 liglfihslf rsgmdqvvvq rylaarklke aqsityigit lnsmynfima gmavalvywy
      301 rdcdpllsga itkldqmlpy yvkenlsdfp gfsglfltgv vsaatstvss iinsqaavfy
      361 vdvisqymkf ttqqaasftk ksalffgcvl tacavavpyl gsavrlilvl hsggtapfvg
      421 ifilglafpw anakgaavgt lvagglqlwl mfgkialdih sprmpvtldy cpvnvsalva
      481 nssasgfdka sslervsgfe ensllsfyrf saywsliftt altiiigvtv slltgekntc
      541 agnfhltskl almlwrklnl lpkpevklpa ekgeeedekn ilcnefkree sfnsneinv
//
LOCUS       XP_077506146             432 aa            linear   INV 25-NOV-2025
DEFINITION  band 7 protein AGAP004871-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077506146
VERSION     XP_077506146.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650020.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..432
                     /product="band 7 protein AGAP004871-like isoform X1"
                     /calculated_mol_wt=45625
     Region          153..400
                     /region_name="SPFH_like"
                     /note="core domain of the SPFH (stomatin, prohibitin,
                     flotillin, and HflK/C) superfamily; cl19107"
                     /db_xref="CDD:473137"
     CDS             1..432
                     /gene="LOC144115601"
                     /coded_by="XM_077650020.1:157..1455"
                     /db_xref="GeneID:144115601"
ORIGIN      
        1 mdsggggypv epaqqlqvps avapaatvpp pvvvppppvv vvsaasgkpp apappilvap
       61 appppvvpih hpamgphlvq dqarysnppg hrwmsqttsa atvsegldic ayilttisil
      121 iiiatfpvsl ifcikvvqey eravifrlgr llrggakgpg iffiipcidt yckvdlrtvs
      181 fdvppqegqa dafraegtpa ylsafvptkt kpppplaedv fygtpplkyt yawilskdsv
      241 tvavdavvyy risnatiavs nvedyghstr llaattlrnv lgtknlseil seresishvm
      301 qasldeatdp wgvkvervei kdvrlpvqlq ramaaeaeaa rearakviaa egeqrasrsl
      361 keaaeviadt psalqlrylq tlasiaaekn stivfpipme lfsgfinsas shghggaggg
      421 ggdvkkdlea aa
//
LOCUS       XP_077506147             419 aa            linear   INV 25-NOV-2025
DEFINITION  band 7 protein AGAP004871-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077506147
VERSION     XP_077506147.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650021.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..419
                     /product="band 7 protein AGAP004871-like isoform X2"
                     /calculated_mol_wt=44977
     Region          140..387
                     /region_name="SPFH_like"
                     /note="core domain of the SPFH (stomatin, prohibitin,
                     flotillin, and HflK/C) superfamily; cl19107"
                     /db_xref="CDD:473137"
     CDS             1..419
                     /gene="LOC144115601"
                     /coded_by="XM_077650021.1:391..1650"
                     /db_xref="GeneID:144115601"
ORIGIN      
        1 mnlepdegfe fgrhaprspa vihkmhpawv pvetltlair gteapaaphq ppqhpppqpp
       61 aplepqgpsa lqyqgkpada pvlpellptv segldicayi lttisiliii atfpvslifc
      121 ikvvqeyera vifrlgrllr ggakgpgiff iipcidtyck vdlrtvsfdv ppqegqadaf
      181 raegtpayls afvptktkpp pplaedvfyg tpplkytyaw ilskdsvtva vdavvyyris
      241 natiavsnve dyghstrlla attlrnvlgt knlseilser esishvmqas ldeatdpwgv
      301 kverveikdv rlpvqlqram aaeaeaarea rakviaaege qrasrslkea aeviadtpsa
      361 lqlrylqtla siaaeknsti vfpipmelfs gfinsasshg hggagggggd vkkdleaaa
//
LOCUS       XP_077506148             386 aa            linear   INV 25-NOV-2025
DEFINITION  band 7 protein AGAP004871-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077506148
VERSION     XP_077506148.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650022.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..386
                     /product="band 7 protein AGAP004871-like isoform X3"
                     /calculated_mol_wt=40611
     Region          153..354
                     /region_name="SPFH_stomatin"
                     /note="Stomatin, a subgroup of the stomatin-like proteins
                     (slipins) family; belonging to the SPFH (stomatin,
                     prohibitin, flotillin, and HflK/C) superfamily; cd03403"
                     /db_xref="CDD:259801"
     CDS             1..386
                     /gene="LOC144115601"
                     /coded_by="XM_077650022.1:156..1316"
                     /db_xref="GeneID:144115601"
ORIGIN      
        1 mdsggggypv epaqqlqvps avapaatvpp pvvvppppvv vvsaasgkpp apappilvap
       61 appppvvpih hpamgphlvq dqarysnppg hrwmsqttsa atvsegldic ayilttisil
      121 iiiatfpvsl ifcikvvqey eravifrlgr llrggakgpg iffiipcidt yckvdlrtvs
      181 fdvppqeils kdsvtvavda vvyyrisnat iavsnvedyg hstrllaatt lrnvlgtknl
      241 seilseresi shvmqaslde atdpwgvkve rveikdvrlp vqlqramaae aeaarearak
      301 viaaegeqra srslkeaaev iadtpsalql rylqtlasia aeknstivfp ipmelfsgfi
      361 nsasshghgg agggggdvkk dleaaa
//
LOCUS       XP_077506149             373 aa            linear   INV 25-NOV-2025
DEFINITION  band 7 protein AGAP004871-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077506149
VERSION     XP_077506149.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650023.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..373
                     /product="band 7 protein AGAP004871-like isoform X4"
                     /calculated_mol_wt=39963
     Region          140..341
                     /region_name="SPFH_like"
                     /note="core domain of the SPFH (stomatin, prohibitin,
                     flotillin, and HflK/C) superfamily; cl19107"
                     /db_xref="CDD:473137"
     CDS             1..373
                     /gene="LOC144115601"
                     /coded_by="XM_077650023.1:409..1530"
                     /db_xref="GeneID:144115601"
ORIGIN      
        1 mnlepdegfe fgrhaprspa vihkmhpawv pvetltlair gteapaaphq ppqhpppqpp
       61 aplepqgpsa lqyqgkpada pvlpellptv segldicayi lttisiliii atfpvslifc
      121 ikvvqeyera vifrlgrllr ggakgpgiff iipcidtyck vdlrtvsfdv ppqeilskds
      181 vtvavdavvy yrisnatiav snvedyghst rllaattlrn vlgtknlsei lseresishv
      241 mqasldeatd pwgvkverve ikdvrlpvql qramaaeaea arearakvia aegeqrasrs
      301 lkeaaeviad tpsalqlryl qtlasiaaek nstivfpipm elfsgfinsa sshghggagg
      361 gggdvkkdle aaa
//
LOCUS       XP_077506152             452 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115602 [Amblyomma americanum].
ACCESSION   XP_077506152
VERSION     XP_077506152.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650026.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..452
                     /product="uncharacterized protein LOC144115602"
                     /calculated_mol_wt=48040
     Region          <176..>305
                     /region_name="KREPA"
                     /note="Kinetoplastid RNA Editing Protein A (KREPA);
                     cl49620"
                     /db_xref="CDD:483960"
     Region          <282..443
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          284..305
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(284,287,300,305)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          313..333
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(313,316,329,333)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          341..361
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(341,344,357,361)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Site            order(346,348,350,352..353,356..357,360,374,376,380..381,
                     384..385,388,402,404,406,408..409,412..413,416)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          369..389
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          397..417
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          425..444
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..452
                     /gene="LOC144115602"
                     /coded_by="XM_077650026.1:92..1450"
                     /db_xref="GeneID:144115602"
ORIGIN      
        1 makspermta aakedggqat aalealaagf gghrvprelg gsafrvvtpk hssftgglpt
       61 lsglprpgnw lldpllpplw piglradqpp plwpflhfyp psdahllspg ggkagaaaae
      121 saklfgtaqg lsalhfrpfa lgsltcdlrv gaavkpwlfa davappppaa pppppglqps
      181 saatsspgaa gqgplgltga asspstppsq aakassrqal spsssepgng glistmapcs
      241 rldlgprlsp qtlsaaglls ahhhhhhlhh lhhlhhplsd vysclkcdkm fstahglevh
      301 arrshsgkrp facelcnktf ghevslsqhr avhtsertfe ckqcgktfkr sstlsthlli
      361 hsdtrpypce ycgkrfhqks dmkkhtyiht gekphkcavc gkafsqssnl ithsrkhtgf
      421 kpfacevcgr afqrkvdlrr hketqhadir pi
//
LOCUS       XP_077506153             624 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115605 [Amblyomma americanum].
ACCESSION   XP_077506153
VERSION     XP_077506153.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650027.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..624
                     /product="uncharacterized protein LOC144115605"
                     /calculated_mol_wt=69008
     Region          484..540
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          560..>623
                     /region_name="rve"
                     /note="Integrase core domain; pfam00665"
                     /db_xref="CDD:459897"
     CDS             1..624
                     /gene="LOC144115605"
                     /coded_by="XM_077650027.1:1..1875"
                     /db_xref="GeneID:144115605"
ORIGIN      
        1 mafsgqktfe svecslvayn vskehwaqlv fpvlaervay lstrftaeqh rdydklkavv
       61 ldvlklsaae yqrrfsgatk rreetwkafa trvqsylnfy lksrcvssfd gllkllvadq
      121 lkaglseeah kyvklregek wlnvdeiaal lqtyeeaagk gsaqkkadsi kgfdtppkpr
      181 teapptrkhg aergdriaav rpggekkkvs rsggcfrcgs wrhvancpfd renpadrren
      241 lprddrltar vsthalqstr palrdikics reavigaivd tgaditvvre slvpeefvsp
      301 hgtidlvsaf gekveaklav vplalcrgap lienvddatp vlctltdklt qadclisada
      361 weqlhdsgkt dseslvqavg tlsppaedrp drsgpdvgte pvlafadetl pqqtvdsnev
      421 epsqaggtae lsgaqkfrae qhedetlrqa wqnakqgkag msivdgvlyh kdqvlgqgvq
      481 qlvlprlrce aalslahesy wggqlgfrkt karlkysfyw pgmesdirkh cdschscqvr
      541 sdhrrtdrvp itpltrpkfp fqkvnadiig piepssgrgh ryalciidly trwpevvclr
      601 slsaratcda llsvfsrtgv pevr
//
LOCUS       XP_077506154             148 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115606 [Amblyomma americanum].
ACCESSION   XP_077506154
VERSION     XP_077506154.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650028.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="uncharacterized protein LOC144115606"
                     /calculated_mol_wt=16606
     Region          <10..42
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..148
                     /gene="LOC144115606"
                     /coded_by="XM_077650028.1:66..512"
                     /db_xref="GeneID:144115606"
ORIGIN      
        1 msghpganta egqynaahas mrcvvercig vlksrfrclq ryrtlhyepe ravviiaaca
       61 alhnicleea laadeddttd dpdnngppll agylagtgsr mtylrgramr driiglfgtt
      121 rpqrqahlqm vrrqqqrqqq qqqqhhrq
//
LOCUS       XP_077506155             940 aa            linear   INV 25-NOV-2025
DEFINITION  piwi-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077506155
VERSION     XP_077506155.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650029.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..940
                     /product="piwi-like protein 1"
                     /calculated_mol_wt=105034
     Region          305..347
                     /region_name="ArgoL1"
                     /note="Argonaute linker 1 domain; pfam08699"
                     /db_xref="CDD:462567"
     Region          368..491
                     /region_name="PAZ"
                     /note="This domain is named PAZ after the proteins Piwi
                     Argonaut and Zwille; smart00949"
                     /db_xref="CDD:198017"
     Site            order(397,410,418,422,440,457,459)
                     /site_type="other"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]"
                     /db_xref="CDD:239211"
     Region          478..923
                     /region_name="Piwi_piwi-like_Euk"
                     /note="PIWI domain, Piwi-like subfamily found in
                     eukaryotes. This domain is found in Piwi and closely
                     related proteins, where it is believed to perform a
                     crucial role in germline cells, via RNA silencing. RNA
                     silencing refers to a group of related...; cd04658"
                     /db_xref="CDD:240016"
     Site            order(645,649,661..664,667,677,680,684,688)
                     /site_type="active"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240016"
     Site            order(709,711,779,915)
                     /site_type="active"
                     /db_xref="CDD:240016"
     CDS             1..940
                     /gene="LOC144115608"
                     /coded_by="XM_077650029.1:135..2957"
                     /db_xref="GeneID:144115608"
ORIGIN      
        1 maskddepya gcgrggrgal lwaalqqqar rpgtedmplp addsgrssgs ssspdsgvss
       61 gshslspfsd sassssspsv pivgrgillg qlmgealssp sppespteql akpgrfsrgi
      121 vkahrqssiv pmpaeqsviv qqrstmqqpd vsgaaratah tearveppta emsklrtkef
      181 rgktgkpvsv evnyvrlgtr qgmgvfeyit dfvpvidskr arckllssds vleligrtrv
      241 fdgaklylph rlrdmvtsvq tvlptdgtsv tvhikfvkit ppsqcmhlyn vlfkkithcl
      301 qltqigrnyf drrgaivvpq hrlevwpgyv qsvaeyeggl llncdvsfrv lrsvtareml
      361 fdvynaypqd ykakavhaiv gavvmtryny rtyrvddiaw dlkptstflf hndemityvd
      421 yykriyniei qdhdqpllmh rgkvrkdape sedarlvclv pelcsmtglt delrsdfrvm
      481 kditahtrvs psqrqrslvk yvksvktcpa avkvlddwgl tlqdcaltld grilpeekim
      541 mrnktyyhng tadwgrllsq dspistinle nwvvvftrrd vertrafvam mskvcpsmgi
      601 qvrapqmrel ssdsidnyvh aikdvakdrv qivvvvfpts rddrysavkr lccvdmpips
      661 qviisntigq qlklrsvtqk valqincklg gelwtvdipm kkvmivgvdv yhdstrggqs
      721 vigfvasmnn nmtrwfskca lqesgkelvn cikvafldal akyyevnhsr pdrififrdg
      781 vgdgqlahvn qyeveqlias fscaspgynp miavvvvqkr intrifakln geveldnpap
      841 gtivdhtitr tdwcdfflvs qkvrqgtvsp thyvvvrnst glspdqmqrl tyklthmyyn
      901 wpgtvrvpap cqyahklasl vgdnihreps pllcdrlfyl
//
LOCUS       XP_077506156             339 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome c oxidase assembly protein COX18, mitochondrial
            [Amblyomma americanum].
ACCESSION   XP_077506156
VERSION     XP_077506156.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650030.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..339
                     /product="cytochrome c oxidase assembly protein COX18,
                     mitochondrial"
                     /calculated_mol_wt=38191
     Region          78..301
                     /region_name="5TM_Oxa1-like"
                     /note="Five transmembrane core domain of mitochondrial
                     inner membrane protein Oxa1 and similar proteins; cd20069"
                     /db_xref="CDD:410993"
     CDS             1..339
                     /gene="LOC144115609"
                     /coded_by="XM_077650030.1:36..1055"
                     /db_xref="GeneID:144115609"
ORIGIN      
        1 mfrfggtrlr rdacstslrf sclvaepkvl lqrgepstyh gvcpvrcfsl qswaqtlsqs
       61 tpvayaqdll daahsasgls wgptivvtsl alrvavtlpl avyqhhvlar fanldremsg
      121 iaqelkrett qavrmfnlne kqarylyrkn fkrqlqrlvv rdnchpmkaa lvvlfqlplw
      181 vslsvalrnm afmmpyqdla aqatylelsv ggmlwftnlt qpdplhiipv lvgianlmni
      241 efhvlqrqkh mawlrrwits fmrglalisv pvawimpadv alywfcssgf algqnmlmaa
      301 prfrracsip vtasesqtpf kdvacrlrer ldaafrtgs
//
LOCUS       XP_077506157             264 aa            linear   INV 25-NOV-2025
DEFINITION  gar1 ribonucleoprotein isoform X1 [Amblyomma americanum].
ACCESSION   XP_077506157
VERSION     XP_077506157.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650031.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..264
                     /product="gar1 ribonucleoprotein isoform X1"
                     /calculated_mol_wt=25807
     Region          71..157
                     /region_name="Gar1"
                     /note="Gar1/Naf1 RNA binding region; pfam04410"
                     /db_xref="CDD:461296"
     CDS             1..264
                     /gene="Gar1"
                     /coded_by="XM_077650031.1:101..895"
                     /db_xref="GeneID:144115611"
ORIGIN      
        1 mvfhrsamnr ggggnfrggr ggfrggsggg frggrggdrg gfggrgggrg gfgggrggfr
       61 ggydqgppee vvefaylshp cqddlvakvt ddrvpffnap lylenkqqig kvdeifgpir
      121 dyfvsvklqd dvkassfskk qkfyidpnkl mplarflpgn qtrgggrrgv ggrgggrggr
      181 ggggpggggf rgrgggfggr gggfggrggg ggfggrgggg gfggrggggg yggrgggggf
      241 ggrggggfgg rggggfggrg fsrs
//
LOCUS       XP_077506158             257 aa            linear   INV 25-NOV-2025
DEFINITION  gar1 ribonucleoprotein isoform X2 [Amblyomma americanum].
ACCESSION   XP_077506158
VERSION     XP_077506158.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650032.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..257
                     /product="gar1 ribonucleoprotein isoform X2"
                     /calculated_mol_wt=24978
     Region          64..150
                     /region_name="Gar1"
                     /note="Gar1/Naf1 RNA binding region; pfam04410"
                     /db_xref="CDD:461296"
     CDS             1..257
                     /gene="Gar1"
                     /coded_by="XM_077650032.1:145..918"
                     /db_xref="GeneID:144115611"
ORIGIN      
        1 mnrggggnfr ggrggfrggs gggfrggrgg drggfggrgg grggfgggrg gfrggydqgp
       61 peevvefayl shpcqddlva kvtddrvpff naplylenkq qigkvdeifg pirdyfvsvk
      121 lqddvkassf skkqkfyidp nklmplarfl pgnqtrgggr rgvggrgggr ggrggggpgg
      181 ggfrgrgggf ggrgggfggr gggggfggrg ggggfggrgg gggyggrggg ggfggrgggg
      241 fggrggggfg grgfsrs
//
LOCUS       XP_077506159             531 aa            linear   INV 25-NOV-2025
DEFINITION  la-related protein 7-like [Amblyomma americanum].
ACCESSION   XP_077506159
VERSION     XP_077506159.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650033.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..531
                     /product="la-related protein 7-like"
                     /calculated_mol_wt=60019
     Region          41..114
                     /region_name="LAM"
                     /note="LA motif RNA-binding domain; cd07323"
                     /db_xref="CDD:153396"
     Site            order(44,47..48,53,56..57,59,80..82)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:153396"
     Region          128..198
                     /region_name="RRM1_LARP7"
                     /note="RNA recognition motif 1 (RRM1) found in La-related
                     protein 7 (LARP7) and similar proteins; cd12290"
                     /db_xref="CDD:409732"
     Region          410..488
                     /region_name="RRM2_LARP7"
                     /note="RNA recognition motif 2 in La-related protein 7
                     (LARP7) and similar proteins; cd12542"
                     /db_xref="CDD:409958"
     CDS             1..531
                     /gene="LOC144115612"
                     /coded_by="XM_077650033.1:184..1779"
                     /db_xref="GeneID:144115612"
ORIGIN      
        1 mgevaqeses tpvqetqepr vtaktekkkv rrrkkklfks irdqmefyfs danlrkdrym
       61 nelvqknkkg yvdlevfltf hkiraltadv kdiagaiats ellqideerk rvrrcaelat
      121 kpdvdectly verlplhadh twlkgvfsrh grvvyvslpr yrhnnrikgf afvefatped
      181 vdracqhfgy ndgnskkpev aarstenddk avpseevkee ksdddepppa krrrevspek
      241 aghgapskda katgegerve ttsdaessda avdkaengds gmelsmsvps kkkkkmrkrk
      301 kktqhsetkv qhsllvmpkc ewkrlrnkyl alqkatmaqi krslllppsg gtpfsspsht
      361 lgspahsvtv ddtdsavpss enrectefsq gngelceket qapvlqfqpg vivkmltekp
      421 vsntsefksn irqlanvayv dvmegdsvay vrcadaegar qfienqkesf cgslsllsgd
      481 eeksywdkin ldrdtrrsqk trqkkrgvek vmakaqkmme kknlhirfdd l
//
LOCUS       XP_077506161             401 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115613 [Amblyomma americanum].
ACCESSION   XP_077506161
VERSION     XP_077506161.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650035.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..401
                     /product="uncharacterized protein LOC144115613"
                     /calculated_mol_wt=43586
     CDS             1..401
                     /gene="LOC144115613"
                     /coded_by="XM_077650035.1:112..1317"
                     /db_xref="GeneID:144115613"
ORIGIN      
        1 misavvfvsl llsqagrsvq qeqdcskiee cygrlrkhfq reldglpgsd ptkqnfveyr
       61 rfcgtlfqsd spcsrfddfk ecskqpgiea rekvyeqvrg fvcadnetsw krfmgikatq
      121 tndackgale atrqvlsgkl decgtvkklf gecaaqqhll tdqletgmdl lgcqqqaptt
      181 papqpgpghh kepqchedea rrclneavmd latsfgfkqg dvmqeqpact dpdqsclqkh
      241 plddcidqsm qlgaiqtgie nvrraacadn rdllkkirkv ascydleafv qclntgnvhi
      301 egtmlspeqc rsilattngc idksrkscan eadasvvekl pstflevyac qpyevpgggd
      361 avsptagvpp akppkgsgvs lsssltlalc valfaafasq h
//
LOCUS       XP_077506162             220 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115615 [Amblyomma americanum].
ACCESSION   XP_077506162
VERSION     XP_077506162.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650036.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..220
                     /product="uncharacterized protein LOC144115615"
                     /calculated_mol_wt=24668
     Region          102..174
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..220
                     /gene="LOC144115615"
                     /coded_by="XM_077650036.1:2691..3353"
                     /db_xref="GeneID:144115615"
ORIGIN      
        1 mdgekrpsre pqrnqaeeds akeshdedgp avdpskpgps miqhiaepqg dswaplvrkr
       61 avedlttrkq pakdrpssta pkriplqdhi peslvydckt mdnhslkltd lpfnvtieev
      121 qelckdavqi qfihdgsllr staryeseqr ameaaealqk amlkgtpipv yhcgarwshl
      181 thsppllhdt lnlhgvpeef siqkhpmvvc gksyihlwll
//
LOCUS       XP_077506163              89 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115617 [Amblyomma americanum].
ACCESSION   XP_077506163
VERSION     XP_077506163.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650037.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..89
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..89
                     /product="uncharacterized protein LOC144115617"
                     /calculated_mol_wt=9793
     Region          7..>71
                     /region_name="UPF0184"
                     /note="Uncharacterized protein family (UPF0184);
                     pfam03670"
                     /db_xref="CDD:112485"
     Region          40..>62
                     /region_name="Peptidase_M14_like"
                     /note="M14 family of metallocarboxypeptidases and related
                     proteins; cl11393"
                     /db_xref="CDD:472171"
     CDS             1..89
                     /gene="LOC144115617"
                     /coded_by="XM_077650037.1:23..292"
                     /db_xref="GeneID:144115617"
ORIGIN      
        1 mareddgpqg dpesnnaeee qdldnvdpaf aqeyealsst mdeinewmdv lerksndlvn
       61 qmrqlieesk qgddlvldsd msagdsaan
//
LOCUS       XP_077506164             910 aa            linear   INV 25-NOV-2025
DEFINITION  sarcosine dehydrogenase, mitochondrial-like [Amblyomma americanum].
ACCESSION   XP_077506164
VERSION     XP_077506164.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650038.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..910
                     /product="sarcosine dehydrogenase, mitochondrial-like"
                     /calculated_mol_wt=100206
     Region          64..429
                     /region_name="DadA"
                     /note="Glycine/D-amino acid oxidase (deaminating) [Amino
                     acid transport and metabolism]; COG0665"
                     /db_xref="CDD:440429"
     Region          416..470
                     /region_name="FAO_M"
                     /note="FAD dependent oxidoreductase central domain;
                     pfam16350"
                     /db_xref="CDD:465100"
     Region          465..900
                     /region_name="GcvT"
                     /note="Glycine cleavage system protein T
                     (aminomethyltransferase) [Amino acid transport and
                     metabolism]; COG0404"
                     /db_xref="CDD:440173"
     CDS             1..910
                     /gene="LOC144115618"
                     /coded_by="XM_077650038.1:561..3293"
                     /db_xref="GeneID:144115618"
ORIGIN      
        1 mvylrkmpvm lkrivppllq vrhqsskits svpyetlerk kvetpatvpa sadvviiggg
       61 iigcstlyhl tklgcssavl lerdrltagt twhtagliwr lrpndtdiqi lnhtrhllmn
      121 vleeetgvhp gfinngglfi anskerldey krlmtigkaf giesyvlspe etkklyplmn
      181 vndlygtlys pgdgtvdpag fctaltraat akgakviekc pvvgiethvd dfgtrrvsgv
      241 qtaagmistk alvnctgawa pavgamvgvp vplvamkhay vvtnriegiq nmpnvrdhdl
      301 slylrlqgda lsvggyepnp ifidqlpedf afglfdldwd vfgthiesgi hrvpvleqtg
      361 ikstvcgpes ftadhkplig eepslrgffh gcgfnslgmn ggggcgrela awvlqgrpnl
      421 dmygydirrf splltkdpkw irershesyv knysivypnd eplasrnmrk dplhevlsqa
      481 gcifqerlgw erpgwfakdg papalaydyy gsygnvkhvs ynyadklkld ytfdfpghhn
      541 iikdeclgcr ksaaifnmsy fgkfyitgpd aqaavdfifs snmqkpkdst tytcmlnsrg
      601 mveadltvsv ispgagspcd pkfdgdgfyv avagasanhc lahirtvvqd qkfkvsiade
      661 setmgmlsiq gpksrellqs ltdsdlsdts flfstnkvit faghkvralr ltfvgemgwe
      721 lhipsvscvp vytaiiqagk afglvnagyr aidslsmekg ykhwhadlrm edtpleagla
      781 ftcklksdtd flgrkalerq kaaglhkria cftldehvpl hgleaiwrdd evvgftrrge
      841 ygfalgksla ygyvrrpdgg pvspeylasg sywleslgrk hkatfhskap fdpankrvrg
      901 iydeplpcds
//
LOCUS       XP_077506165             910 aa            linear   INV 25-NOV-2025
DEFINITION  sarcosine dehydrogenase, mitochondrial-like [Amblyomma americanum].
ACCESSION   XP_077506165
VERSION     XP_077506165.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650039.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..910
                     /product="sarcosine dehydrogenase, mitochondrial-like"
                     /calculated_mol_wt=100206
     Region          64..429
                     /region_name="DadA"
                     /note="Glycine/D-amino acid oxidase (deaminating) [Amino
                     acid transport and metabolism]; COG0665"
                     /db_xref="CDD:440429"
     Region          416..470
                     /region_name="FAO_M"
                     /note="FAD dependent oxidoreductase central domain;
                     pfam16350"
                     /db_xref="CDD:465100"
     Region          465..900
                     /region_name="GcvT"
                     /note="Glycine cleavage system protein T
                     (aminomethyltransferase) [Amino acid transport and
                     metabolism]; COG0404"
                     /db_xref="CDD:440173"
     CDS             1..910
                     /gene="LOC144115618"
                     /coded_by="XM_077650039.1:153..2885"
                     /db_xref="GeneID:144115618"
ORIGIN      
        1 mvylrkmpvm lkrivppllq vrhqsskits svpyetlerk kvetpatvpa sadvviiggg
       61 iigcstlyhl tklgcssavl lerdrltagt twhtagliwr lrpndtdiqi lnhtrhllmn
      121 vleeetgvhp gfinngglfi anskerldey krlmtigkaf giesyvlspe etkklyplmn
      181 vndlygtlys pgdgtvdpag fctaltraat akgakviekc pvvgiethvd dfgtrrvsgv
      241 qtaagmistk alvnctgawa pavgamvgvp vplvamkhay vvtnriegiq nmpnvrdhdl
      301 slylrlqgda lsvggyepnp ifidqlpedf afglfdldwd vfgthiesgi hrvpvleqtg
      361 ikstvcgpes ftadhkplig eepslrgffh gcgfnslgmn ggggcgrela awvlqgrpnl
      421 dmygydirrf splltkdpkw irershesyv knysivypnd eplasrnmrk dplhevlsqa
      481 gcifqerlgw erpgwfakdg papalaydyy gsygnvkhvs ynyadklkld ytfdfpghhn
      541 iikdeclgcr ksaaifnmsy fgkfyitgpd aqaavdfifs snmqkpkdst tytcmlnsrg
      601 mveadltvsv ispgagspcd pkfdgdgfyv avagasanhc lahirtvvqd qkfkvsiade
      661 setmgmlsiq gpksrellqs ltdsdlsdts flfstnkvit faghkvralr ltfvgemgwe
      721 lhipsvscvp vytaiiqagk afglvnagyr aidslsmekg ykhwhadlrm edtpleagla
      781 ftcklksdtd flgrkalerq kaaglhkria cftldehvpl hgleaiwrdd evvgftrrge
      841 ygfalgksla ygyvrrpdgg pvspeylasg sywleslgrk hkatfhskap fdpankrvrg
      901 iydeplpcds
//
LOCUS       XP_077506166             438 aa            linear   INV 25-NOV-2025
DEFINITION  ATP-dependent RNA helicase 25E isoform X1 [Amblyomma americanum].
ACCESSION   XP_077506166
VERSION     XP_077506166.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650040.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..438
                     /product="ATP-dependent RNA helicase 25E isoform X1"
                     /calculated_mol_wt=50140
     Region          55..262
                     /region_name="DEADc_DDX39"
                     /note="DEAD-box helicase domain of DEAD box protein 39;
                     cd17950"
                     /db_xref="CDD:350708"
     Site            order(58,76,78..80,83,102..108,207..208)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350708"
     Region          273..402
                     /region_name="SF2_C_DEAD"
                     /note="C-terminal helicase domain of the DEAD box
                     helicases; cd18787"
                     /db_xref="CDD:350174"
     Site            order(361,363,388,391..392)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350174"
     CDS             1..438
                     /gene="Hel25E"
                     /coded_by="XM_077650040.1:150..1466"
                     /db_xref="GeneID:144115620"
ORIGIN      
        1 myyqevyvie pvaqrsamad ndlldyeded dqdqtagdvi pevkkptggy vsihssgfrd
       61 fllkpellra ivdcgfehps evqhecipqa ilgmdilcqa ksgmgktavf vlatlqqldp
      121 iegqvsvlvm chtrelafqi skeyerfsky lpsvrvgvff ggmnitndek vlksscphvv
      181 vgtpgrvlal vrsrklqlkh ikhfvldecd kmleqldmrr dvqeifrstp hekqvmmfsa
      241 tlskdirpvc lkfmqdpmev yvddeakltl hglqqyyvkl kdneknrklf elldllefnq
      301 vvifvktvqr cmalaqllve qnfpaiaihr amtqeerlsr yqqfkdfqkr ilvatnlfgr
      361 gmdiervniv fnydmpedsd tylhrvarag rfgtkglavt fvsdetdakt lnevqdrfdv
      421 niselpdeid issyiegr
//
LOCUS       XP_077506167             421 aa            linear   INV 25-NOV-2025
DEFINITION  ATP-dependent RNA helicase 25E isoform X2 [Amblyomma americanum].
ACCESSION   XP_077506167
VERSION     XP_077506167.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650041.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..421
                     /product="ATP-dependent RNA helicase 25E isoform X2"
                     /calculated_mol_wt=48112
     Region          38..245
                     /region_name="DEADc_DDX39"
                     /note="DEAD-box helicase domain of DEAD box protein 39;
                     cd17950"
                     /db_xref="CDD:350708"
     Site            order(41,59,61..63,66,85..91,190..191)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350708"
     Region          256..385
                     /region_name="SF2_C_DEAD"
                     /note="C-terminal helicase domain of the DEAD box
                     helicases; cd18787"
                     /db_xref="CDD:350174"
     Site            order(344,346,371,374..375)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350174"
     CDS             1..421
                     /gene="Hel25E"
                     /coded_by="XM_077650041.1:88..1353"
                     /db_xref="GeneID:144115620"
ORIGIN      
        1 madndlldye deddqdqtag dvipevkkpt ggyvsihssg frdfllkpel lraivdcgfe
       61 hpsevqheci pqailgmdil cqaksgmgkt avfvlatlqq ldpiegqvsv lvmchtrela
      121 fqiskeyerf skylpsvrvg vffggmnitn dekvlksscp hvvvgtpgrv lalvrsrklq
      181 lkhikhfvld ecdkmleqld mrrdvqeifr stphekqvmm fsatlskdir pvclkfmqdp
      241 mevyvddeak ltlhglqqyy vklkdneknr klfelldlle fnqvvifvkt vqrcmalaql
      301 lveqnfpaia ihramtqeer lsryqqfkdf qkrilvatnl fgrgmdierv nivfnydmpe
      361 dsdtylhrva ragrfgtkgl avtfvsdetd aktlnevqdr fdvniselpd eidissyieg
      421 r
//
LOCUS       XP_077506168             105 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: notch-regulated ankyrin repeat-containing
            protein-like [Amblyomma americanum].
ACCESSION   XP_077506168
VERSION     XP_077506168.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650042.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..105
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..105
                     /product="LOW QUALITY PROTEIN: notch-regulated ankyrin
                     repeat-containing protein-like"
                     /calculated_mol_wt=11515
     Region          <15..>100
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(17..19,21..22,26,29,42,44,46,50..51,54..56,58..59,
                     63,66,75,77,79,83..84,87..89,91..92,96,99)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          44..75
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..105
                     /gene="LOC144115622"
                     /coded_by="XM_077650042.1:74..391"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:144115622"
ORIGIN      
        1 msqteivsqe vfqravkkgd aselkrllqr weggslnvnl ydhegqtalh ksvlegnlel
       61 vkllvkfgad trlanrdgws aihiaayggh qdialylisn sggrr
//
LOCUS       XP_077506169             457 aa            linear   INV 25-NOV-2025
DEFINITION  monocarboxylate transporter 12-B-like [Amblyomma americanum].
ACCESSION   XP_077506169
VERSION     XP_077506169.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650043.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..457
                     /product="monocarboxylate transporter 12-B-like"
                     /calculated_mol_wt=49197
     Region          46..438
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(77,128..129,131..133,136,155,158..159,162,269,
                     272..273,276..278,281,308,312,364..365,369,373,389,
                     392..393,396..397,400)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:349949"
     CDS             1..457
                     /gene="LOC144115623"
                     /coded_by="XM_077650043.1:113..1486"
                     /db_xref="GeneID:144115623"
ORIGIN      
        1 mampqtrpar kgaaregpds krawylgvfc tayaffgwvg lsstpvlyvg likysgasre
       61 tasqpftamm cayftgsily gglahwfneq klllaaatii sstlfasyfi sditlltvvl
      121 gvvhglgvas vgvvvgalls qyfikyrata falmsvtlsl tgvvfppvaa yfvaeygfst
      181 sllllgavsl hlflcclpla papwqreppp ppprpatlta pknpavrent nghipvqdes
      241 kqntpkwtiv lkkvrpflqg ifpiccttna cgcfaiftyt ltivdfahdc gfqgyraaml
      301 mtamsvgwav asvslapavd aglttketvv ftsfvvqglg fifmgllgqh yawlvtgsvl
      361 vgwgqgsrgf llfvfvperf pkgqvpaafa lmslsctlpf farapiigyv rdtlgsyvml
      421 mvgmavmevv lavlwgalal lkwrhrtakl rialpfa
//
LOCUS       XP_077506170             457 aa            linear   INV 25-NOV-2025
DEFINITION  monocarboxylate transporter 12-B-like [Amblyomma americanum].
ACCESSION   XP_077506170
VERSION     XP_077506170.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650044.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..457
                     /product="monocarboxylate transporter 12-B-like"
                     /calculated_mol_wt=49197
     Region          46..438
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(77,128..129,131..133,136,155,158..159,162,269,
                     272..273,276..278,281,308,312,364..365,369,373,389,
                     392..393,396..397,400)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:349949"
     CDS             1..457
                     /gene="LOC144115623"
                     /coded_by="XM_077650044.1:226..1599"
                     /db_xref="GeneID:144115623"
ORIGIN      
        1 mampqtrpar kgaaregpds krawylgvfc tayaffgwvg lsstpvlyvg likysgasre
       61 tasqpftamm cayftgsily gglahwfneq klllaaatii sstlfasyfi sditlltvvl
      121 gvvhglgvas vgvvvgalls qyfikyrata falmsvtlsl tgvvfppvaa yfvaeygfst
      181 sllllgavsl hlflcclpla papwqreppp ppprpatlta pknpavrent nghipvqdes
      241 kqntpkwtiv lkkvrpflqg ifpiccttna cgcfaiftyt ltivdfahdc gfqgyraaml
      301 mtamsvgwav asvslapavd aglttketvv ftsfvvqglg fifmgllgqh yawlvtgsvl
      361 vgwgqgsrgf llfvfvperf pkgqvpaafa lmslsctlpf farapiigyv rdtlgsyvml
      421 mvgmavmevv lavlwgalal lkwrhrtakl rialpfa
//
LOCUS       XP_077506171             457 aa            linear   INV 25-NOV-2025
DEFINITION  monocarboxylate transporter 12-B-like [Amblyomma americanum].
ACCESSION   XP_077506171
VERSION     XP_077506171.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650045.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..457
                     /product="monocarboxylate transporter 12-B-like"
                     /calculated_mol_wt=49197
     Region          46..438
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(77,128..129,131..133,136,155,158..159,162,269,
                     272..273,276..278,281,308,312,364..365,369,373,389,
                     392..393,396..397,400)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:349949"
     CDS             1..457
                     /gene="LOC144115623"
                     /coded_by="XM_077650045.1:327..1700"
                     /db_xref="GeneID:144115623"
ORIGIN      
        1 mampqtrpar kgaaregpds krawylgvfc tayaffgwvg lsstpvlyvg likysgasre
       61 tasqpftamm cayftgsily gglahwfneq klllaaatii sstlfasyfi sditlltvvl
      121 gvvhglgvas vgvvvgalls qyfikyrata falmsvtlsl tgvvfppvaa yfvaeygfst
      181 sllllgavsl hlflcclpla papwqreppp ppprpatlta pknpavrent nghipvqdes
      241 kqntpkwtiv lkkvrpflqg ifpiccttna cgcfaiftyt ltivdfahdc gfqgyraaml
      301 mtamsvgwav asvslapavd aglttketvv ftsfvvqglg fifmgllgqh yawlvtgsvl
      361 vgwgqgsrgf llfvfvperf pkgqvpaafa lmslsctlpf farapiigyv rdtlgsyvml
      421 mvgmavmevv lavlwgalal lkwrhrtakl rialpfa
//
LOCUS       XP_077506172             457 aa            linear   INV 25-NOV-2025
DEFINITION  monocarboxylate transporter 12-B-like [Amblyomma americanum].
ACCESSION   XP_077506172
VERSION     XP_077506172.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650046.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..457
                     /product="monocarboxylate transporter 12-B-like"
                     /calculated_mol_wt=49197
     Region          46..438
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(77,128..129,131..133,136,155,158..159,162,269,
                     272..273,276..278,281,308,312,364..365,369,373,389,
                     392..393,396..397,400)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:349949"
     CDS             1..457
                     /gene="LOC144115623"
                     /coded_by="XM_077650046.1:179..1552"
                     /db_xref="GeneID:144115623"
ORIGIN      
        1 mampqtrpar kgaaregpds krawylgvfc tayaffgwvg lsstpvlyvg likysgasre
       61 tasqpftamm cayftgsily gglahwfneq klllaaatii sstlfasyfi sditlltvvl
      121 gvvhglgvas vgvvvgalls qyfikyrata falmsvtlsl tgvvfppvaa yfvaeygfst
      181 sllllgavsl hlflcclpla papwqreppp ppprpatlta pknpavrent nghipvqdes
      241 kqntpkwtiv lkkvrpflqg ifpiccttna cgcfaiftyt ltivdfahdc gfqgyraaml
      301 mtamsvgwav asvslapavd aglttketvv ftsfvvqglg fifmgllgqh yawlvtgsvl
      361 vgwgqgsrgf llfvfvperf pkgqvpaafa lmslsctlpf farapiigyv rdtlgsyvml
      421 mvgmavmevv lavlwgalal lkwrhrtakl rialpfa
//
LOCUS       XP_077506173             457 aa            linear   INV 25-NOV-2025
DEFINITION  monocarboxylate transporter 12-B-like [Amblyomma americanum].
ACCESSION   XP_077506173
VERSION     XP_077506173.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650047.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..457
                     /product="monocarboxylate transporter 12-B-like"
                     /calculated_mol_wt=49197
     Region          46..438
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(77,128..129,131..133,136,155,158..159,162,269,
                     272..273,276..278,281,308,312,364..365,369,373,389,
                     392..393,396..397,400)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:349949"
     CDS             1..457
                     /gene="LOC144115623"
                     /coded_by="XM_077650047.1:150..1523"
                     /db_xref="GeneID:144115623"
ORIGIN      
        1 mampqtrpar kgaaregpds krawylgvfc tayaffgwvg lsstpvlyvg likysgasre
       61 tasqpftamm cayftgsily gglahwfneq klllaaatii sstlfasyfi sditlltvvl
      121 gvvhglgvas vgvvvgalls qyfikyrata falmsvtlsl tgvvfppvaa yfvaeygfst
      181 sllllgavsl hlflcclpla papwqreppp ppprpatlta pknpavrent nghipvqdes
      241 kqntpkwtiv lkkvrpflqg ifpiccttna cgcfaiftyt ltivdfahdc gfqgyraaml
      301 mtamsvgwav asvslapavd aglttketvv ftsfvvqglg fifmgllgqh yawlvtgsvl
      361 vgwgqgsrgf llfvfvperf pkgqvpaafa lmslsctlpf farapiigyv rdtlgsyvml
      421 mvgmavmevv lavlwgalal lkwrhrtakl rialpfa
//
LOCUS       XP_077506174             759 aa            linear   INV 25-NOV-2025
DEFINITION  putative ATP-dependent RNA helicase Dbp73D [Amblyomma americanum].
ACCESSION   XP_077506174
VERSION     XP_077506174.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650048.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..759
                     /product="putative ATP-dependent RNA helicase Dbp73D"
                     /calculated_mol_wt=84556
     Region          282..523
                     /region_name="DEADc_DDX51"
                     /note="DEAD-box helicase domain of DEAD box protein 51;
                     cd17956"
                     /db_xref="CDD:350714"
     Site            order(290..291,293..295,298,325..332)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350714"
     Region          552..679
                     /region_name="SF2_C_DEAD"
                     /note="C-terminal helicase domain of the DEAD box
                     helicases; cd18787"
                     /db_xref="CDD:350174"
     Site            order(639,641,666,669..670)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350174"
     CDS             1..759
                     /gene="Dbp73D"
                     /coded_by="XM_077650048.1:4912..7191"
                     /db_xref="GeneID:144115624"
ORIGIN      
        1 meklyvrftd daecagerav dpdkrlrkil kkakrrqeea rhlarddcll vgqaevahen
       61 irrdrekrki gdrgceleqd ndvapvgkka rpgskdqgig tshavpeass dtamveapkg
      121 kkktrrkrsq qirvrrdrrk nltvglqvdg dlspkaerae dseevrknlt pelqvdgdlq
      181 prtersedrq hkptasdddv gamefadten gapddlrepa evisreyypv lgdvqvkkie
      241 avhrvlpewl snpevvqsiv kkgkkpgsak venftsslsa emlsvlatnn irklfpvqek
      301 vvpwllspeq rrshlpprdi cvsaptgsgk tlayvipive alkgrvvrav ravvvlpvke
      361 lavqvhavfl qyvratslnv qlvtgtrtfa eeqallvrkt arglvsladi vvatpgrlld
      421 hvrktpgfnl hllkffvlde adrviedvqt tlipeveqav fgadklnccc kepvhgrlht
      481 hplticclqn crepvqklly satltqdpdk lqslmlfqpk lftataalsv egvtqrqktf
      541 vgkyttpqgl seyyyvtldn tkplavwdlv anhgfrdtlc ftaskedahr lslvlkemgg
      601 vraeefsakl ssaerarvlr kfasgkldil vcsnvlargl dvpnvrhvic ydpprfikty
      661 vhrvgrtara gvpgtavtfm rqgqlqafka mlesagkqdi qpldtsesdg leefqskyrd
      721 alkavevivk seqngpergk kysfaqkswa akeapdvqh
//
LOCUS       XP_077506175             406 aa            linear   INV 25-NOV-2025
DEFINITION  prostaglandin E synthase Su(P) [Amblyomma americanum].
ACCESSION   XP_077506175
VERSION     XP_077506175.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650049.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..406
                     /product="prostaglandin E synthase Su(P)"
                     /calculated_mol_wt=45673
     Region          120..196
                     /region_name="GST_N_mPGES2"
                     /note="GST_N family; microsomal Prostaglandin E synthase
                     Type 2 (mPGES2) subfamily; mPGES2 is a membrane-anchored
                     dimeric protein containing a CXXC motif which catalyzes
                     the isomerization of PGH2 to PGE2. Unlike cytosolic PGE
                     synthase (cPGES) and microsomal...; cd03040"
                     /db_xref="CDD:239338"
     Site            order(126,128..132)
                     /site_type="active"
                     /note="GRX-like active site [active]"
                     /db_xref="CDD:239338"
     Site            order(127..129,133..134,136..137,141,152..154,158,185,189)
                     /site_type="other"
                     /note="C-terminal domain interface [polypeptide binding]"
                     /db_xref="CDD:239338"
     Site            order(166,182..183,185..186,189)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:239338"
     Region          242..394
                     /region_name="GST_C_mPGES2"
                     /note="C-terminal, alpha helical domain of microsomal
                     Prostaglandin E synthase Type 2; cd03197"
                     /db_xref="CDD:198306"
     Site            order(243,247,254,259,318,320)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:198306"
     Site            order(253,257,281..282,285,352,361,364..365)
                     /site_type="other"
                     /note="N-terminal domain interface [polypeptide binding]"
                     /db_xref="CDD:198306"
     Site            order(264..265,268..269,278,281..282,365,369)
                     /site_type="active"
                     /note="GRX-like active site [active]"
                     /db_xref="CDD:198306"
     CDS             1..406
                     /gene="Su(P)"
                     /coded_by="XM_077650049.1:5..1225"
                     /db_xref="GeneID:144115625"
ORIGIN      
        1 mpdwhrqptr lamaalsaql arcaslakcf spatayasvh aaklssfars ssklnssklr
       61 flavaglasg aighaymrsl elrkdpigdi fsplhtksit hpgthisrvv ngpttktglk
      121 itmyqyqtcp fcckvrafld fygipynvve vdpvlrqqlk fsdykkvpil lveeggncwq
      181 iydstviism lqsylrdikg gfrkylclye pvkvtdasgk eslevfnkyf lmmdsapaqg
      241 kalielkeeq twrmwaddvl vhvlspnvyr twqealqafn yfsevgewer nfptwerllv
      301 iyvgaaamyf vgkrlkkrhn lkddvresfr dacrewtaav gterkfhggs hpnladlavy
      361 gvlssvegcp afedmltdtk igdwyyrmke aisshagtra lsamtc
//
LOCUS       XP_077506176             140 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115626 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506176
VERSION     XP_077506176.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650050.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..140
                     /product="uncharacterized protein LOC144115626 isoform X1"
                     /calculated_mol_wt=15984
     CDS             1..140
                     /gene="LOC144115626"
                     /coded_by="XM_077650050.1:149..571"
                     /db_xref="GeneID:144115626"
ORIGIN      
        1 myfelilrtr nldvprdgwi ankchlmlda cqlfwfpghr gpeqqramsa rcnttedspf
       61 rqcrtaarlq rlpvpaaegm sweqadvdrp avlravlrhq ratalawplv alarsrtpdl
      121 amasqwdqkq ahrvphhpdl
//
LOCUS       XP_077506177             114 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115626 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506177
VERSION     XP_077506177.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650051.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..114
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..114
                     /product="uncharacterized protein LOC144115626 isoform X2"
                     /calculated_mol_wt=12828
     CDS             1..114
                     /gene="LOC144115626"
                     /coded_by="XM_077650051.1:320..664"
                     /db_xref="GeneID:144115626"
ORIGIN      
        1 mldacqlfwf pghrgpeqqr amsarcntte dspfrqcrta arlqrlpvpa aegmsweqad
       61 vdrpavlrav lrhqratala wplvalarsr tpdlamasqw dqkqahrvph hpdl
//
LOCUS       XP_077506178              92 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115626 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077506178
VERSION     XP_077506178.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650052.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..92
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..92
                     /product="uncharacterized protein LOC144115626 isoform X3"
                     /calculated_mol_wt=10486
     CDS             1..92
                     /gene="LOC144115626"
                     /coded_by="XM_077650052.1:135..413"
                     /db_xref="GeneID:144115626"
ORIGIN      
        1 myfelilrtr nldvprdgwi ankchlmlda gmsweqadvd rpavlravlr hqratalawp
       61 lvalarsrtp dlamasqwdq kqahrvphhp dl
//
LOCUS       XP_077506179             981 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115628 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506179
VERSION     XP_077506179.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650053.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..981
                     /product="uncharacterized protein LOC144115628 isoform X1"
                     /calculated_mol_wt=106821
     Region          905..975
                     /region_name="cupin_RmlC-like"
                     /note="RmlC-like cupin superfamily; cl40423"
                     /db_xref="CDD:477354"
     CDS             1..981
                     /gene="LOC144115628"
                     /coded_by="XM_077650053.1:357..3302"
                     /db_xref="GeneID:144115628"
ORIGIN      
        1 maeqharlma dkpvldgstt rnarkdhklf icsgledtft fstgdiplfq dgkanlqrdl
       61 dcpalqsksa enspclttey irdlqtsggs pvnilmsqhc ssiletsptk tdvqaliavp
      121 kcspvvsfet sdppagisgg ssdqpeqclp aidssaidss aidfsaidss aidfsaidfs
      181 aidslaidsl tpekqhspkg qkeglklcdp vkgsgndksa adclgaqksa dvnqdgklcq
      241 shstrgekkk qllardiafc ftpnfpaspe esrcpelkkq rqtrtsvtse svgartplth
      301 ishfniqrqi tdnivsktkn tkmvqkhrsq klpkwlstst kenalclanl kqktkeretl
      361 lttkssdkgk qdceslpssl gspppsksss rkkpnqvplm srdlwssttc nslqrpntds
      421 eqalddlasn vhtnrchsmq vrdveqdsll sagtkdvtqs ahpnnisdkd mqaaenhcdn
      481 gqhvdqnlrt dcfssselsl psvtppkqss lrdksarspv lgskisfqds qrpekrsgsv
      541 paksglqhek lpvqkenfws qpravscqas qvhnkevqke stvslnaats rrkshssgqg
      601 acgkemkasr tspskagsfa mkgeahvrre tegtapcsnh lssgtephvs kallaadgsa
      661 gavrswknfw hssrnvvkta tgqlpvvsaa kerlsdkape enysksqkke ggrqcfplge
      721 kqdvpkassi adcfrepvmp rkpfwhclag fsastqhrks evkgmraved kqlpavcear
      781 gkfsdkrpng gsskaelyhe kkkqctshta kcsevkacpg rtvskqielq dglqhevkda
      841 svqtsfvcmi gsrakmdrea rhrlvsslyv gedkvkfldp nlrpagesap fsfgyyaatd
      901 svimgimklr pdcekplavd yvhdmcfyvl ddglmvstvg dawtllekgs vfrllsgmpy
      961 sfknvgtats klfyvlmkpr t
//
LOCUS       XP_077506180             931 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115628 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506180
VERSION     XP_077506180.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650054.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..931
                     /product="uncharacterized protein LOC144115628 isoform X2"
                     /calculated_mol_wt=101348
     Region          855..925
                     /region_name="cupin_RmlC-like"
                     /note="RmlC-like cupin superfamily; cl40423"
                     /db_xref="CDD:477354"
     CDS             1..931
                     /gene="LOC144115628"
                     /coded_by="XM_077650054.1:46..2841"
                     /db_xref="GeneID:144115628"
ORIGIN      
        1 megctgcrll aalthprrth mgsqkpmgni tvtirvtnec tgdrtrsqhc ssiletsptk
       61 tdvqaliavp kcspvvsfet sdppagisgg ssdqpeqclp aidssaidss aidfsaidss
      121 aidfsaidfs aidslaidsl tpekqhspkg qkeglklcdp vkgsgndksa adclgaqksa
      181 dvnqdgklcq shstrgekkk qllardiafc ftpnfpaspe esrcpelkkq rqtrtsvtse
      241 svgartplth ishfniqrqi tdnivsktkn tkmvqkhrsq klpkwlstst kenalclanl
      301 kqktkeretl lttkssdkgk qdceslpssl gspppsksss rkkpnqvplm srdlwssttc
      361 nslqrpntds eqalddlasn vhtnrchsmq vrdveqdsll sagtkdvtqs ahpnnisdkd
      421 mqaaenhcdn gqhvdqnlrt dcfssselsl psvtppkqss lrdksarspv lgskisfqds
      481 qrpekrsgsv paksglqhek lpvqkenfws qpravscqas qvhnkevqke stvslnaats
      541 rrkshssgqg acgkemkasr tspskagsfa mkgeahvrre tegtapcsnh lssgtephvs
      601 kallaadgsa gavrswknfw hssrnvvkta tgqlpvvsaa kerlsdkape enysksqkke
      661 ggrqcfplge kqdvpkassi adcfrepvmp rkpfwhclag fsastqhrks evkgmraved
      721 kqlpavcear gkfsdkrpng gsskaelyhe kkkqctshta kcsevkacpg rtvskqielq
      781 dglqhevkda svqtsfvcmi gsrakmdrea rhrlvsslyv gedkvkfldp nlrpagesap
      841 fsfgyyaatd svimgimklr pdcekplavd yvhdmcfyvl ddglmvstvg dawtllekgs
      901 vfrllsgmpy sfknvgtats klfyvlmkpr t
//
LOCUS       XP_077506181             437 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115629 [Amblyomma americanum].
ACCESSION   XP_077506181
VERSION     XP_077506181.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650055.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..437
                     /product="uncharacterized protein LOC144115629"
                     /calculated_mol_wt=49454
     Region          <157..>274
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..437
                     /gene="LOC144115629"
                     /coded_by="XM_077650055.1:877..2190"
                     /db_xref="GeneID:144115629"
ORIGIN      
        1 mrppnplcff vqvtawalfs naplsspthe isklpchfnd tcdatcpqdr hifiavppsh
       61 ersrstatsf aaymnswrap galsghenst rmrppnplcf fvqvssvtsl yakksndvcl
      121 llfprpgvll cvfsecfdiv nqllalsgdv elnpgpntrh qvqdslpdvq skqledmfai
      181 letfntrtak mqldqkafik siddlkksql kleedlsemn krltnverkt vtidetekev
      241 hpfqqlvdql ssensqlrar lseledrqrr dnllfygipd aqseswaqse eklvkvlssc
      301 leipsseili drahrlggfa sekcrpiiak fssfkvkqri llncsklkde kitlsedfsf
      361 atrharkklv efgksqpltf klrfnklyik nkcysynhsd dsvheipsaf plptdrsedp
      421 mndthsasap ptelvtn
//
LOCUS       XP_077506182             263 aa            linear   INV 25-NOV-2025
DEFINITION  peroxiredoxin-6-like [Amblyomma americanum].
ACCESSION   XP_077506182
VERSION     XP_077506182.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650056.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) and transcript sequence (DAPXPI010019610.1) annotated
            using gene prediction method: Gnomon, supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            assembly gap :: added 301 transcript bases to patch genome assembly
                            gap
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Amblyomma americanum"
                     /db_xref="taxon:6943"
                     /chromosome="1"
     Protein         1..263
                     /product="peroxiredoxin-6-like"
                     /calculated_mol_wt=28777
     Region          48..261
                     /region_name="PRX_1cys"
                     /note="Peroxiredoxin (PRX) family, 1-cys PRX subfamily;
                     composed of PRXs containing only one conserved cysteine,
                     which serves as the peroxidatic cysteine. They are
                     homodimeric thiol-specific antioxidant (TSA) proteins that
                     confer a protective role in cells by...; cd03016"
                     /db_xref="CDD:239314"
     Site            order(48,86,89..91,94,126,129,155,170,184..186,188..190,
                     192..197,199,202,206..207,216..217,219..220,257,260)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239314"
     Site            order(60,114..116,118..119,121..122,124..125,137,146..147,
                     157,168..169,232,255)
                     /site_type="other"
                     /note="decamer (pentamer of dimers) interface [polypeptide
                     binding]"
                     /db_xref="CDD:239314"
     Site            order(85,88,172)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:239314"
     CDS             1..263
                     /gene="LOC144115630"
                     /coded_by="XM_077650056.1:5..796"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: added 301 bases not found in
                     genome assembly"
                     /db_xref="GeneID:144115630"
ORIGIN      
        1 mraghqekgv cfgaliqlsp vhpatasrph ftsdqraaaa akmpslnlgd pfpnftcett
       61 vgtidfhewl gnswailfsh padytpvctt elaraaqlah vfeekgvkli alscdsvesh
      121 hgwikdieaf gelpdgpfpy piiadekrei avqlgmldpv ekdkeglplt cravfiigpd
      181 kkmklsmlyp attgrnfdev lratdsllvt etrkvatpag wkkgtpcmvl psvtdeeipk
      241 lfptgikeyq vpsgkkylrt tmd
//
LOCUS       XP_077506183             228 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115631 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506183
VERSION     XP_077506183.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650057.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..228
                     /product="uncharacterized protein LOC144115631 isoform X1"
                     /calculated_mol_wt=25653
     CDS             1..228
                     /gene="LOC144115631"
                     /coded_by="XM_077650057.1:417..1103"
                     /db_xref="GeneID:144115631"
ORIGIN      
        1 msplsrfgav lvcacsayia ehnninrtma atcswfsghg kskktgvsaf kvekamydtv
       61 kripidavfh krtaipiwst eavvlcsssg ncsggdngsg pfllckqrls frrfahhqyc
      121 slyvpcqihr rmpqkrrdtg rkaclkkvka rtenskkker nmnlrpevap ylhkvshnmk
      181 kvanrhcgyt agvkllsgkf nlfdfarkda rydarglhld ytanfvyq
//
LOCUS       XP_077506184             228 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115631 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506184
VERSION     XP_077506184.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650058.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..228
                     /product="uncharacterized protein LOC144115631 isoform X1"
                     /calculated_mol_wt=25653
     CDS             1..228
                     /gene="LOC144115631"
                     /coded_by="XM_077650058.1:417..1103"
                     /db_xref="GeneID:144115631"
ORIGIN      
        1 msplsrfgav lvcacsayia ehnninrtma atcswfsghg kskktgvsaf kvekamydtv
       61 kripidavfh krtaipiwst eavvlcsssg ncsggdngsg pfllckqrls frrfahhqyc
      121 slyvpcqihr rmpqkrrdtg rkaclkkvka rtenskkker nmnlrpevap ylhkvshnmk
      181 kvanrhcgyt agvkllsgkf nlfdfarkda rydarglhld ytanfvyq
//
LOCUS       XP_077506185             197 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115631 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506185
VERSION     XP_077506185.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650059.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..197
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..197
                     /product="uncharacterized protein LOC144115631 isoform X2"
                     /calculated_mol_wt=21559
     CDS             1..197
                     /gene="LOC144115631"
                     /coded_by="XM_077650059.1:417..1010"
                     /db_xref="GeneID:144115631"
ORIGIN      
        1 msplsrfgav lvcacsayia ehnninrtma atcswfsghg kskktgvsaf kvekamydtv
       61 kripidavfh krtaipiwst eavvlcsssg ncsggdngsg pfllckqrls frrfahhqyc
      121 slyvpcqihr rmpqkrrdtg rsivatqles scyqgnstys tlpekmhamm lgasiltilp
      181 ilstndakda fvqisks
//
LOCUS       XP_077506186             495 aa            linear   INV 25-NOV-2025
DEFINITION  5-phosphohydroxy-L-lysine phospho-lyase isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506186
VERSION     XP_077506186.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650060.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..495
                     /product="5-phosphohydroxy-L-lysine phospho-lyase isoform
                     X1"
                     /calculated_mol_wt=54897
     Region          <14..447
                     /region_name="PRK06148"
                     /note="hypothetical protein; Provisional"
                     /db_xref="CDD:180426"
     CDS             1..495
                     /gene="LOC144115636"
                     /coded_by="XM_077650060.1:186..1673"
                     /db_xref="GeneID:144115636"
ORIGIN      
        1 metsapssle kickeetirl rdiyvgksct vffksngplk ivrgqgqymy desgnayldc
       61 innvahvghc hphvvkagse qmallstnsr ylhdnlvlya qrltnyfpnk lsvcyfvnsg
      121 seandlamrl arcytknkdi icldsayhgh lqsttaispy kfnhiigwqk kksvhvaplp
      181 ctyrgkyrdc dypagtdfgq myaqevlnli nqthldgrri aafiheslvs cggqfplpkn
      241 ylrhvyqyvr eaggvciade vqtgfgrvge hmwgfqlqge dicpdivtlg kpignghpia
      301 mvvttrevan rfeaqgaeyf ntyggnpvsv avasavldvi eneklqqhak evgaylmycl
      361 relqqrhpli gdvrghglfv gvelvldrqt repageqasl lslrlkqqgv llstegkygn
      421 vlkfkppmvf srenvdtvvs kldillgkie decldlrynf advahslass veslstassf
      481 dsleesseed lssss
//
LOCUS       XP_077506187             447 aa            linear   INV 25-NOV-2025
DEFINITION  ethanolamine-phosphate phospho-lyase isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506187
VERSION     XP_077506187.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650061.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..447
                     /product="ethanolamine-phosphate phospho-lyase isoform X2"
                     /calculated_mol_wt=49505
     Region          <1..399
                     /region_name="PRK06148"
                     /note="hypothetical protein; Provisional"
                     /db_xref="CDD:180426"
     CDS             1..447
                     /gene="LOC144115636"
                     /coded_by="XM_077650061.1:856..2199"
                     /db_xref="GeneID:144115636"
ORIGIN      
        1 mydesgnayl dcinnvahvg hchphvvkag seqmallstn srylhdnlvl yaqrltnyfp
       61 nklsvcyfvn sgseandlam rlarcytknk diicldsayh ghlqsttais pykfnhiigw
      121 qkkksvhvap lpctyrgkyr dcdypagtdf gqmyaqevln linqthldgr riaafihesl
      181 vscggqfplp knylrhvyqy vreaggvcia devqtgfgrv gehmwgfqlq gedicpdivt
      241 lgkpignghp iamvvttrev anrfeaqgae yfntyggnpv svavasavld vieneklqqh
      301 akevgaylmy clrelqqrhp ligdvrghgl fvgvelvldr qtrepageqa sllslrlkqq
      361 gvllstegky gnvlkfkppm vfsrenvdtv vskldillgk iedecldlry nfadvahsla
      421 ssveslstas sfdsleesse edlssss
//
LOCUS       XP_077506188             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115637 [Amblyomma americanum].
ACCESSION   XP_077506188
VERSION     XP_077506188.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650062.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144115637"
                     /calculated_mol_wt=14331
     CDS             1..127
                     /gene="LOC144115637"
                     /coded_by="XM_077650062.1:442..825"
                     /db_xref="GeneID:144115637"
ORIGIN      
        1 mvralhsdrr iaaykpfrrl sdqlprrdra lllrlrigct wtpsrlytcd rahspacpsc
       61 gdtgtlghll laypthdppr rrlesgyral glpsttehdl lfpdrsqlla hrallsflee
      121 tglsifl
//
LOCUS       XP_077506190             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115637 [Amblyomma americanum].
ACCESSION   XP_077506190
VERSION     XP_077506190.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650064.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144115637"
                     /calculated_mol_wt=14331
     CDS             1..127
                     /gene="LOC144115637"
                     /coded_by="XM_077650064.1:1714..2097"
                     /db_xref="GeneID:144115637"
ORIGIN      
        1 mvralhsdrr iaaykpfrrl sdqlprrdra lllrlrigct wtpsrlytcd rahspacpsc
       61 gdtgtlghll laypthdppr rrlesgyral glpsttehdl lfpdrsqlla hrallsflee
      121 tglsifl
//
LOCUS       XP_077506191             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115637 [Amblyomma americanum].
ACCESSION   XP_077506191
VERSION     XP_077506191.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650065.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144115637"
                     /calculated_mol_wt=14331
     CDS             1..127
                     /gene="LOC144115637"
                     /coded_by="XM_077650065.1:435..818"
                     /db_xref="GeneID:144115637"
ORIGIN      
        1 mvralhsdrr iaaykpfrrl sdqlprrdra lllrlrigct wtpsrlytcd rahspacpsc
       61 gdtgtlghll laypthdppr rrlesgyral glpsttehdl lfpdrsqlla hrallsflee
      121 tglsifl
//
LOCUS       XP_077506192             127 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115637 [Amblyomma americanum].
ACCESSION   XP_077506192
VERSION     XP_077506192.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650066.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..127
                     /product="uncharacterized protein LOC144115637"
                     /calculated_mol_wt=14331
     CDS             1..127
                     /gene="LOC144115637"
                     /coded_by="XM_077650066.1:249..632"
                     /db_xref="GeneID:144115637"
ORIGIN      
        1 mvralhsdrr iaaykpfrrl sdqlprrdra lllrlrigct wtpsrlytcd rahspacpsc
       61 gdtgtlghll laypthdppr rrlesgyral glpsttehdl lfpdrsqlla hrallsflee
      121 tglsifl
//
LOCUS       XP_077506193             889 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077506193
VERSION     XP_077506193.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650067.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..889
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=100197
     Region          20..>173
                     /region_name="PHA03255"
                     /note="BDLF3; Provisional"
                     /db_xref="CDD:165513"
     Region          210..335
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          429..811
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..889
                     /gene="LOC144115638"
                     /coded_by="XM_077650067.1:410..3079"
                     /db_xref="GeneID:144115638"
ORIGIN      
        1 mepkqlrqir lprcsvlfll mlachtptaq casvpaqaei ftgvpassli spaeaeatty
       61 psttrifrvs ppntfstlpq spsildgtta ktketalpss tqrevhagta assrttrsts
      121 lfdgtearts tseesvnaat tsittsasdt rsakavqehq ttgvteaptt rqperskfld
      181 nvramirqvm ssmgsgltrk llqadistdc slgvlkfmra vqdlepwalr lfdstakypn
      241 gmlqastadl gaydecietv vrdeegvekv rgqycnvhvs lgeerslige llpamlyshe
      301 raqnftayit derlpglrlg vcfinscneq dltnigrtla gtvakivvkn cvtnvdegin
      361 tsqawiiafl tllastivaa tvfehltedw dkkrknaiay kctvafsals nsrlimsvnk
      421 dqnsetysyr fmhgirflsi fwiclghsyg titenitrlv nalhyferwe tlivtagyla
      481 vdsffffsgf llyytlskqk qnrilvavva mirrfiratf pmffmimciy lmpliasgpn
      541 skefynkfyt emrnhwwdll fqlrnwradq evstmphlwy lsadyqfflv gvvviqifrt
      601 rkwlvaiift vlsivccgis awqiygtnmt pflvavsstf stvmdtlnhy ymlpfyhgvc
      661 ffsgcitfli verygkakis kmiqaslwci slfcglyclf mklqwyrsde rateikrify
      721 aftdrifwsv cvawfvftca tgrgglvnrf lswdgfvpls rlsfgvylih spfyilmyhi
      781 arervffshf tlvsqcfavl vwsyilsyfl fiacdaptgh lekllfmser rkdhsqtvaa
      841 ngvsnnntrd lpvgiieegn iknyfngaap clgggttcdg sytnkgcrl
//
LOCUS       XP_077506194             531 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115639 [Amblyomma americanum].
ACCESSION   XP_077506194
VERSION     XP_077506194.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650068.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..531
                     /product="uncharacterized protein LOC144115639"
                     /calculated_mol_wt=60892
     Region          6..81
                     /region_name="THAP"
                     /note="The THAP domain is a putative DNA-binding domain
                     (DBD) and probably also binds a zinc ion; smart00980"
                     /db_xref="CDD:214951"
     Region          <82..>243
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <157..>235
                     /region_name="COG4913"
                     /note="Uncharacterized conserved protein, contains a
                     C-terminal ATPase domain [Function unknown]"
                     /db_xref="CDD:443941"
     Region          365..524
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..531
                     /gene="LOC144115639"
                     /coded_by="XM_077650068.1:86..1681"
                     /db_xref="GeneID:144115639"
ORIGIN      
        1 mggftccvpg cynnsardkg lgfyvfpkdh klrqtwlqri nragqtgrfs kfvpttghrv
       61 csahfeggkk tymvrvpsif plrqqkvekr rlltrtepev sststvcteq rgqevsafas
      121 ssnesdcrqp evnilmdhsy ssqkfsydel vkkfceqkhi atdlleqaet anaqvaelkr
      181 qlersrldha etkklcdrlq qknrclrlel ssmqrkvktc kaeaaqnien tyeelvkdek
      241 klryytgfts serleafwsf lepdakrlqf wqmketnsed rkfilplktq lvlvlmrlrl
      301 gldgldlayr favskttvsr mwatwldfld nrlcqvptwm ppelcdkyrp kafsdkgytt
      361 vdgildctei fietpssfrv qsetystykk hntakglvvc spngfvtfvs dlapgrvsdk
      421 altnssgvle kfapgrsvma drgftieeec kqrsldlnip pflegrpqls eedenetrli
      481 asvrihverv irkiktyrml sqvfpnsmsg qlnkvwhica lltnfvgepl l
//
LOCUS       XP_077506195             195 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115640 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506195
VERSION     XP_077506195.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650069.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..195
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..195
                     /product="uncharacterized protein LOC144115640 isoform X1"
                     /calculated_mol_wt=20893
     CDS             1..195
                     /gene="LOC144115640"
                     /coded_by="XM_077650069.1:709..1296"
                     /db_xref="GeneID:144115640"
ORIGIN      
        1 mtaqrvtili amakvfvqii tfaflpvwaa rppgivpdir tphqegelca rhlvatdtwe
       61 pletpplenw tkqaasaaps sptapfasat kqrqppsahc glcgcatart anplifvmqe
      121 pyqttgrwgg ssrptnqvps rvavhlsdpv psaayqlptp ragfagtark hrrqsfthrr
      181 lclqgdisnt aaapa
//
LOCUS       XP_077506196             195 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115640 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506196
VERSION     XP_077506196.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650070.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..195
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..195
                     /product="uncharacterized protein LOC144115640 isoform X1"
                     /calculated_mol_wt=20893
     CDS             1..195
                     /gene="LOC144115640"
                     /coded_by="XM_077650070.1:691..1278"
                     /db_xref="GeneID:144115640"
ORIGIN      
        1 mtaqrvtili amakvfvqii tfaflpvwaa rppgivpdir tphqegelca rhlvatdtwe
       61 pletpplenw tkqaasaaps sptapfasat kqrqppsahc glcgcatart anplifvmqe
      121 pyqttgrwgg ssrptnqvps rvavhlsdpv psaayqlptp ragfagtark hrrqsfthrr
      181 lclqgdisnt aaapa
//
LOCUS       XP_077506197             195 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115640 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506197
VERSION     XP_077506197.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650071.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..195
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..195
                     /product="uncharacterized protein LOC144115640 isoform X1"
                     /calculated_mol_wt=20893
     CDS             1..195
                     /gene="LOC144115640"
                     /coded_by="XM_077650071.1:1318..1905"
                     /db_xref="GeneID:144115640"
ORIGIN      
        1 mtaqrvtili amakvfvqii tfaflpvwaa rppgivpdir tphqegelca rhlvatdtwe
       61 pletpplenw tkqaasaaps sptapfasat kqrqppsahc glcgcatart anplifvmqe
      121 pyqttgrwgg ssrptnqvps rvavhlsdpv psaayqlptp ragfagtark hrrqsfthrr
      181 lclqgdisnt aaapa
//
LOCUS       XP_077506198             195 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115640 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506198
VERSION     XP_077506198.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650072.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..195
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..195
                     /product="uncharacterized protein LOC144115640 isoform X1"
                     /calculated_mol_wt=20893
     CDS             1..195
                     /gene="LOC144115640"
                     /coded_by="XM_077650072.1:685..1272"
                     /db_xref="GeneID:144115640"
ORIGIN      
        1 mtaqrvtili amakvfvqii tfaflpvwaa rppgivpdir tphqegelca rhlvatdtwe
       61 pletpplenw tkqaasaaps sptapfasat kqrqppsahc glcgcatart anplifvmqe
      121 pyqttgrwgg ssrptnqvps rvavhlsdpv psaayqlptp ragfagtark hrrqsfthrr
      181 lclqgdisnt aaapa
//
LOCUS       XP_077506199             195 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115640 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506199
VERSION     XP_077506199.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650073.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..195
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..195
                     /product="uncharacterized protein LOC144115640 isoform X1"
                     /calculated_mol_wt=20893
     CDS             1..195
                     /gene="LOC144115640"
                     /coded_by="XM_077650073.1:669..1256"
                     /db_xref="GeneID:144115640"
ORIGIN      
        1 mtaqrvtili amakvfvqii tfaflpvwaa rppgivpdir tphqegelca rhlvatdtwe
       61 pletpplenw tkqaasaaps sptapfasat kqrqppsahc glcgcatart anplifvmqe
      121 pyqttgrwgg ssrptnqvps rvavhlsdpv psaayqlptp ragfagtark hrrqsfthrr
      181 lclqgdisnt aaapa
//
LOCUS       XP_077506200             187 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115640 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506200
VERSION     XP_077506200.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650074.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="uncharacterized protein LOC144115640 isoform X2"
                     /calculated_mol_wt=20090
     CDS             1..187
                     /gene="LOC144115640"
                     /coded_by="XM_077650074.1:709..1272"
                     /db_xref="GeneID:144115640"
ORIGIN      
        1 mtaqrvtili amakvfvqii tfaflpvwaa rppgivpdir tphqegelca rhlvatdtwe
       61 pletpplenw tkqaasaaps sptapfasat kqrqppsahc glcgcatart anplifvmqe
      121 pyqttgrwgg ssrptnqiac pvlsrtatfs gsffpltvvd wnaiptdiaa ithpsvfsdt
      181 vgnyfkk
//
LOCUS       XP_077506201             177 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115640 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077506201
VERSION     XP_077506201.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650075.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..177
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..177
                     /product="uncharacterized protein LOC144115640 isoform X3"
                     /calculated_mol_wt=18764
     CDS             1..177
                     /gene="LOC144115640"
                     /coded_by="XM_077650075.1:709..1242"
                     /db_xref="GeneID:144115640"
ORIGIN      
        1 mtaqrvtili amakvfvqii tfaflpvwaa rppgivpdir tphqegelca rhlvatdtwe
       61 pletpplenw tkqaasaaps sptapfasat kqrqppsahc glcgcatart anplifvmqe
      121 pyqttgrwgg ssrptnqvll gllgntggnp spidgyacrv tsaiplqhlp dcstrgi
//
LOCUS       XP_077506203             191 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115640 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077506203
VERSION     XP_077506203.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650077.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..191
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..191
                     /product="uncharacterized protein LOC144115640 isoform X4"
                     /calculated_mol_wt=20326
     CDS             1..191
                     /gene="LOC144115640"
                     /coded_by="XM_077650077.1:1313..1888"
                     /db_xref="GeneID:144115640"
ORIGIN      
        1 msvislaftl ksvsqlghml fgadrckppg ivpdirtphq egelcarhlv atdtweplet
       61 pplenwtkqa asaapsspta pfasatkqrq ppsahcglcg catartanpl ifvmqepyqt
      121 tgrwggssrp tnqvpsrvav hlsdpvpsaa yqlptpragf agtarkhrrq sfthrrlclq
      181 gdisntaaap a
//
LOCUS       XP_077506204             169 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115640 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077506204
VERSION     XP_077506204.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650078.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..169
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..169
                     /product="uncharacterized protein LOC144115640 isoform X5"
                     /calculated_mol_wt=18028
     CDS             1..169
                     /gene="LOC144115640"
                     /coded_by="XM_077650078.1:940..1449"
                     /db_xref="GeneID:144115640"
ORIGIN      
        1 meahrppgiv pdirtphqeg elcarhlvat dtwepletpp lenwtkqaas aapssptapf
       61 asatkqrqpp sahcglcgca tartanplif vmqepyqttg rwggssrptn qvpsrvavhl
      121 sdpvpsaayq lptpragfag tarkhrrqsf thrrlclqgd isntaaapa
//
LOCUS       XP_077506205             159 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115640 isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077506205
VERSION     XP_077506205.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650079.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..159
                     /product="uncharacterized protein LOC144115640 isoform X6"
                     /calculated_mol_wt=16760
     CDS             1..159
                     /gene="LOC144115640"
                     /coded_by="XM_077650079.1:709..1188"
                     /db_xref="GeneID:144115640"
ORIGIN      
        1 mtaqrvtili amakvfvqii tfaflpvwaa rppgivpdir tphqegelca rhlvatdtwe
       61 pletpplenw tkqaasaaps sptapfasat kqrqppsahc glcgcatart anplifvmqv
      121 llgllgntgg npspidgyac rvtsaiplqh lpdcstrgi
//
LOCUS       XP_077506206             936 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115642 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506206
VERSION     XP_077506206.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650080.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..936
                     /product="uncharacterized protein LOC144115642 isoform X1"
                     /calculated_mol_wt=103550
     Region          55..167
                     /region_name="EST1"
                     /note="Telomerase activating protein Est1; pfam10374"
                     /db_xref="CDD:463062"
     Region          154..180
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(156..157,160..161,163,187,190..191,194..195,
                     197..198,221,224..225,228..229,232)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          171..435
                     /region_name="EST1_DNA_bind"
                     /note="Est1 DNA/RNA binding domain; pfam10373"
                     /db_xref="CDD:431239"
     Region          185..215
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     CDS             1..936
                     /gene="LOC144115642"
                     /coded_by="XM_077650080.1:141..2951"
                     /db_xref="GeneID:144115642"
ORIGIN      
        1 mkasvseilk eaesvksala ednprnaraf hlrtrlcwll egllltdlef aldkkveqel
       61 wnsafrnpia afqaksadkk erangesqtm laalletasg fylqlfrklc fmydisfkch
      121 lkdpsygilh ldkhgdlnve kpkkssvlyi cqhclvhlgd iaryrgqslq aenfyrhave
      181 mgprnghpyn qlalleaasg eklsavffhv rslalqhpfp aaatnlsklf skmvsdscis
      241 igkskmtpye yttaflqfha hiylltdmsq aegvlgslts clpalvmvek letwqllqmv
      301 aicmfnqehc tqvsgstdss taalqkkaqh llldlvasml hafllpmhal qeprdmrdcc
      361 svpvvkpvld wlvhkqellh sepfvrkpqi whgfckllna lrvvlpaefp kdkyftvplp
      421 edwelqcfmp lfdahselhf esaptlmkke agdlrasrll diaevlsqvi mngknvisls
      481 kdtparyvan hyteamrnqv mkqiqqlsie pegdsrksai dlrrgalqkr dmnksgsseg
      541 aalevrfkld gdktgepekr gpkgmasmke rrpgrnvamq aifqqreqrs asladshsgr
      601 rdasadavst stvhnihsrs shktdekpwy hqaqllphnl vssngnrgnf rlpnptngqp
      661 lngptapltf dpgvapmppp qsqqlfgmie gggqqaagqq npsfvpggla glaapgvgla
      721 apgvgpsylw nyyvpqpmat pgapqpapll ylgtgqpmas hqlesllrqq qqhqqqqqql
      781 qqqqlqqqqq qqlqqlqqql qqqqlqeqqa dlepnkmasg pdlfqgnpvs amatppsqpp
      841 wvpphtmevn gcevvppgga aqtsgsdlpt ntyslfnspw psglhhmske ggkspagaid
      901 pmvmhsriqs lwsgpgpspl erlleqqklw rdgaaq
//
LOCUS       XP_077506207             922 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115642 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506207
VERSION     XP_077506207.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650081.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..922
                     /product="uncharacterized protein LOC144115642 isoform X2"
                     /calculated_mol_wt=101926
     Region          55..167
                     /region_name="EST1"
                     /note="Telomerase activating protein Est1; pfam10374"
                     /db_xref="CDD:463062"
     Region          154..180
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(156..157,160..161,163,187,190..191,194..195,
                     197..198,221,224..225,228..229,232)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          171..435
                     /region_name="EST1_DNA_bind"
                     /note="Est1 DNA/RNA binding domain; pfam10373"
                     /db_xref="CDD:431239"
     Region          185..215
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     CDS             1..922
                     /gene="LOC144115642"
                     /coded_by="XM_077650081.1:141..2909"
                     /db_xref="GeneID:144115642"
ORIGIN      
        1 mkasvseilk eaesvksala ednprnaraf hlrtrlcwll egllltdlef aldkkveqel
       61 wnsafrnpia afqaksadkk erangesqtm laalletasg fylqlfrklc fmydisfkch
      121 lkdpsygilh ldkhgdlnve kpkkssvlyi cqhclvhlgd iaryrgqslq aenfyrhave
      181 mgprnghpyn qlalleaasg eklsavffhv rslalqhpfp aaatnlsklf skmvsdscis
      241 igkskmtpye yttaflqfha hiylltdmsq aegvlgslts clpalvmvek letwqllqmv
      301 aicmfnqehc tqvsgstdss taalqkkaqh llldlvasml hafllpmhal qeprdmrdcc
      361 svpvvkpvld wlvhkqellh sepfvrkpqi whgfckllna lrvvlpaefp kdkyftvplp
      421 edwelqcfmp lfdahselhf esaptlmkke agdlrasrll diaevlsqvi mngknvisls
      481 kdtparyvan hyteamrnqv mkqiqqlsie pegdsrksdk sgssegaale vrfkldgdkt
      541 gepekrgpkg masmkerrpg rnvamqaifq qreqrsasla dshsgrrdas adavststvh
      601 nihsrsshkt dekpwyhqaq llphnlvssn gnrgnfrlpn ptngqplngp tapltfdpgv
      661 apmpppqsqq lfgmiegggq qaagqqnpsf vpgglaglaa pgvglaapgv gpsylwnyyv
      721 pqpmatpgap qpapllylgt gqpmashqle sllrqqqqhq qqqqqlqqqq lqqqqqqqlq
      781 qlqqqlqqqq lqeqqadlep nkmasgpdlf qgnpvsamat ppsqppwvpp htmevngcev
      841 vppggaaqts gsdlptntys lfnspwpsgl hhmskeggks pagaidpmvm hsriqslwsg
      901 pgpsplerll eqqklwrdga aq
//
LOCUS       XP_077506208             901 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115642 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077506208
VERSION     XP_077506208.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650082.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..901
                     /product="uncharacterized protein LOC144115642 isoform X3"
                     /calculated_mol_wt=99811
     Region          55..167
                     /region_name="EST1"
                     /note="Telomerase activating protein Est1; pfam10374"
                     /db_xref="CDD:463062"
     Region          154..180
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(156..157,160..161,163,187,190..191,194..195,
                     197..198,221,224..225,228..229,232)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          171..435
                     /region_name="EST1_DNA_bind"
                     /note="Est1 DNA/RNA binding domain; pfam10373"
                     /db_xref="CDD:431239"
     Region          185..215
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     CDS             1..901
                     /gene="LOC144115642"
                     /coded_by="XM_077650082.1:141..2846"
                     /db_xref="GeneID:144115642"
ORIGIN      
        1 mkasvseilk eaesvksala ednprnaraf hlrtrlcwll egllltdlef aldkkveqel
       61 wnsafrnpia afqaksadkk erangesqtm laalletasg fylqlfrklc fmydisfkch
      121 lkdpsygilh ldkhgdlnve kpkkssvlyi cqhclvhlgd iaryrgqslq aenfyrhave
      181 mgprnghpyn qlalleaasg eklsavffhv rslalqhpfp aaatnlsklf skmvsdscis
      241 igkskmtpye yttaflqfha hiylltdmsq aegvlgslts clpalvmvek letwqllqmv
      301 aicmfnqehc tqvsgstdss taalqkkaqh llldlvasml hafllpmhal qeprdmrdcc
      361 svpvvkpvld wlvhkqellh sepfvrkpqi whgfckllna lrvvlpaefp kdkyftvplp
      421 edwelqcfmp lfdahselhf esaptlmkke agdlrasrll diaevlsqvi mngknvisls
      481 kdtparyvan hyteamrnqv mkqiqqlsie pegdsrksai dlrrgalqkr dmnksgsseg
      541 aalevrfkld gdktgepekr gpkgmasmke rrpgrnvamq ihsrsshktd ekpwyhqaql
      601 lphnlvssng nrgnfrlpnp tngqplngpt apltfdpgva pmpppqsqql fgmiegggqq
      661 aagqqnpsfv pgglaglaap gvglaapgvg psylwnyyvp qpmatpgapq papllylgtg
      721 qpmashqles llrqqqqhqq qqqqlqqqql qqqqqqqlqq lqqqlqqqql qeqqadlepn
      781 kmasgpdlfq gnpvsamatp psqppwvpph tmevngcevv ppggaaqtsg sdlptntysl
      841 fnspwpsglh hmskeggksp agaidpmvmh sriqslwsgp gpsplerlle qqklwrdgaa
      901 q
//
LOCUS       XP_077506209             187 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115643 [Amblyomma americanum].
ACCESSION   XP_077506209
VERSION     XP_077506209.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650083.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="uncharacterized protein LOC144115643"
                     /calculated_mol_wt=21178
     CDS             1..187
                     /gene="LOC144115643"
                     /coded_by="XM_077650083.1:843..1406"
                     /db_xref="GeneID:144115643"
ORIGIN      
        1 mrerrkikar sgaspdelyq pkwahykslm fldtscssqp sfstlhtqee eeeterqceh
       61 wnnesadqem dfpcssqnca epslpaqqig vgtsrpgkkr rqnddeteqr rvllttavet
      121 lqrsqqrlaa ndecdafgsm mahavrqipp gpdrqlamln ahqaivkinl srhsvpveml
      181 nvlnmqe
//
LOCUS       XP_077506210             151 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115645 [Amblyomma americanum].
ACCESSION   XP_077506210
VERSION     XP_077506210.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650084.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..151
                     /product="uncharacterized protein LOC144115645"
                     /calculated_mol_wt=17043
     CDS             1..151
                     /gene="LOC144115645"
                     /coded_by="XM_077650084.1:356..811"
                     /db_xref="GeneID:144115645"
ORIGIN      
        1 mlywqmnwdw wecrlrtgwq npfhhvaksg crvspqakaq prpmrqpmlp hskppqplar
       61 rngmsgtcte rvvaihrayw aclavsvqyy lcgrqfdlva lpvpgvgvra vksilssspn
      121 lqlngvatew pmtctqrvti liamakdgnw e
//
LOCUS       XP_077506211             111 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115646 [Amblyomma americanum].
ACCESSION   XP_077506211
VERSION     XP_077506211.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650085.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..111
                     /product="uncharacterized protein LOC144115646"
                     /calculated_mol_wt=12237
     CDS             1..111
                     /gene="LOC144115646"
                     /coded_by="XM_077650085.1:300..635"
                     /db_xref="GeneID:144115646"
ORIGIN      
        1 mqfsgcsvtt micklepflq atqdkhirgt lfdpsltlrk rkkdvsgklp vespthatlv
       61 sdretrltmd kmtkgsgqkr ipedevaadl rillahshra qramcgdssd l
//
LOCUS       XP_077506212             665 aa            linear   INV 25-NOV-2025
DEFINITION  carnitine O-acetyltransferase-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506212
VERSION     XP_077506212.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650086.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..665
                     /product="carnitine O-acetyltransferase-like isoform X1"
                     /calculated_mol_wt=74602
     Region          75..648
                     /region_name="Carn_acyltransf"
                     /note="Choline/Carnitine o-acyltransferase; pfam00755"
                     /db_xref="CDD:459926"
     CDS             1..665
                     /gene="LOC144115647"
                     /coded_by="XM_077650086.1:16..2013"
                     /db_xref="GeneID:144115647"
ORIGIN      
        1 msckqtrpeg kevqnrrnsr tgrtsmraem lirgslacar gkmvnefskh vslrpaasav
       61 qmgpmmskqr elkklpvppl ketlqryves vkplvtagql ektlkvvqef gkpngigekl
      121 ykvleskaas tenwladwwl qgaylsyrmp lvvysnpama lpkqtfmqke dqlkhaalli
      181 agaldfkalv erqqfepemm gkdpldmsqy qkifstcrvp gykqdglaty flsdnppkhv
      241 lvvhnneffa ldvydgqgnp ydehqilqql hqvvnmstsa gagigiltte hrdfwakvfd
      301 elcqdprnae svefikkail vvcldqklkv kdpyevacay qmllggrhge naanrwcdkt
      361 vqlivgedgy tgllyehsps egppvavlvd hcykymyvhs dkkqgcpqss kaadnmprkl
      421 efhlsadivk diekarssls gltadldlfi ykykgygkef ikscklspds yiqmamqlay
      481 ykihrapgat yesastrkfv hgrtetirst sseslefcka fesgetklpe kverlrkavq
      541 nhkdytnlai ngcgvdrllf glkmiaiene ipvpeiykda aykistefki sssqvatqcd
      601 avmfygplvk dgygccynpq ahivifglss fnssaatsta kfrdaleesl tqmhdclrls
      661 qaskl
//
LOCUS       XP_077506213             661 aa            linear   INV 25-NOV-2025
DEFINITION  carnitine O-acetyltransferase-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506213
VERSION     XP_077506213.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650087.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..661
                     /product="carnitine O-acetyltransferase-like isoform X2"
                     /calculated_mol_wt=74098
     Region          75..644
                     /region_name="Carn_acyltransf"
                     /note="Choline/Carnitine o-acyltransferase; pfam00755"
                     /db_xref="CDD:459926"
     CDS             1..661
                     /gene="LOC144115647"
                     /coded_by="XM_077650087.1:16..2001"
                     /db_xref="GeneID:144115647"
ORIGIN      
        1 msckqtrpeg kevqnrrnsr tgrtsmraem lirgslacar gkmvnefskh vslrpaasav
       61 qmgpmmskqr elkklpvppl ketlqryves vkplvtagql ektlkvvqef gkpngigekl
      121 ykvleskaas tenwladwwl qgaylsyrmp lvvysnpama lpkqtfmqke dqlkhaalli
      181 agaldfkalv erqqfepemm gkdpldmsqy qkifstcrvp gykqdglaty flsdnppkhv
      241 lvvhnneffa ldvydgqgnp ydehqilqql hqvvnmstsa gagigiltte hrdfwakvfd
      301 elcqdprnae svefikkail vvcldqklkv kdpyevacay qmllggrhge naanrwcdkt
      361 vqlivgedgy tgllyehsps egppvavlvd hcykyidkkq gcpqsskaad nmprklefhl
      421 sadivkdiek arsslsglta dldlfiykyk gygkefiksc klspdsyiqm amqlayykih
      481 rapgatyesa strkfvhgrt etirstsses lefckafesg etklpekver lrkavqnhkd
      541 ytnlaingcg vdrllfglkm iaieneipvp eiykdaayki stefkisssq vatqcdavmf
      601 ygplvkdgyg ccynpqahiv ifglssfnss aatstakfrd aleesltqmh dclrlsqask
      661 l
//
LOCUS       XP_077506214             594 aa            linear   INV 25-NOV-2025
DEFINITION  carnitine O-acetyltransferase-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077506214
VERSION     XP_077506214.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650088.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..594
                     /product="carnitine O-acetyltransferase-like isoform X3"
                     /calculated_mol_wt=66911
     Region          75..594
                     /region_name="Carn_acyltransf"
                     /note="Choline/Carnitine o-acyltransferase; pfam00755"
                     /db_xref="CDD:459926"
     CDS             1..594
                     /gene="LOC144115647"
                     /coded_by="XM_077650088.1:16..1800"
                     /db_xref="GeneID:144115647"
ORIGIN      
        1 msckqtrpeg kevqnrrnsr tgrtsmraem lirgslacar gkmvnefskh vslrpaasav
       61 qmgpmmskqr elkklpvppl ketlqryves vkplvtagql ektlkvvqef gkpngigekl
      121 ykvleskaas tenwladwwl qgaylsyrmp lvvysnpama lpkqtfmqke dqlkhaalli
      181 agaldfkalv erqqfepemm gkdpldmsqy qkifstcrvp gykqdglaty flsdnppkhv
      241 lvvhnneffa ldvydgqgnp ydehqilqql hqvvnmstsa gagigiltte hrdfwakvfd
      301 elcqdprnae svefikkail vvcldqklkv kdpyevacay qmllggrhge naanrwcdkt
      361 vqlivgedgy tgllyehsps egppvavlvd hcykymyvhs dkkqgcpqss kaadnmprkl
      421 efhlsadivk diekarssls gltadldlfi ykykgygkef ikscklspds yiqmamqlay
      481 ykihrapgat yesastrkfv hgrtetirst sseslefcka fesgetklpe kverlrkavq
      541 nhkdytnlai ngcgvdrllf glkmiaiene ipvpeiykda aykistefki sssq
//
LOCUS       XP_077506215             590 aa            linear   INV 25-NOV-2025
DEFINITION  carnitine O-acetyltransferase-like isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077506215
VERSION     XP_077506215.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650089.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..590
                     /product="carnitine O-acetyltransferase-like isoform X4"
                     /calculated_mol_wt=66407
     Region          75..590
                     /region_name="Carn_acyltransf"
                     /note="Choline/Carnitine o-acyltransferase; pfam00755"
                     /db_xref="CDD:459926"
     CDS             1..590
                     /gene="LOC144115647"
                     /coded_by="XM_077650089.1:16..1788"
                     /db_xref="GeneID:144115647"
ORIGIN      
        1 msckqtrpeg kevqnrrnsr tgrtsmraem lirgslacar gkmvnefskh vslrpaasav
       61 qmgpmmskqr elkklpvppl ketlqryves vkplvtagql ektlkvvqef gkpngigekl
      121 ykvleskaas tenwladwwl qgaylsyrmp lvvysnpama lpkqtfmqke dqlkhaalli
      181 agaldfkalv erqqfepemm gkdpldmsqy qkifstcrvp gykqdglaty flsdnppkhv
      241 lvvhnneffa ldvydgqgnp ydehqilqql hqvvnmstsa gagigiltte hrdfwakvfd
      301 elcqdprnae svefikkail vvcldqklkv kdpyevacay qmllggrhge naanrwcdkt
      361 vqlivgedgy tgllyehsps egppvavlvd hcykyidkkq gcpqsskaad nmprklefhl
      421 sadivkdiek arsslsglta dldlfiykyk gygkefiksc klspdsyiqm amqlayykih
      481 rapgatyesa strkfvhgrt etirstsses lefckafesg etklpekver lrkavqnhkd
      541 ytnlaingcg vdrllfglkm iaieneipvp eiykdaayki stefkisssq
//
LOCUS       XP_077506216             123 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial ribosomal protein L35 [Amblyomma americanum].
ACCESSION   XP_077506216
VERSION     XP_077506216.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650090.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..123
                     /product="mitochondrial ribosomal protein L35"
                     /calculated_mol_wt=14646
     Region          35..91
                     /region_name="Ribosomal_L35p"
                     /note="Ribosomal protein L35; pfam01632"
                     /db_xref="CDD:460273"
     CDS             1..123
                     /gene="mRpL35"
                     /coded_by="XM_077650090.1:189..560"
                     /db_xref="GeneID:144115650"
ORIGIN      
        1 mhrgprpllq lapvaaleqs rsitkfsmrn gqrrtckaav krfmrlncgl wirprsgrak
       61 nlwkkpdhvl eglrthvfcs rtqckmldkm vgdywkrpky yvddsyepyh ernnflhakk
      121 tww
//
LOCUS       XP_077506218             786 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077506218
VERSION     XP_077506218.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650092.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..786
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=87060
     Region          96..212
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          307..701
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..786
                     /gene="LOC144115651"
                     /coded_by="XM_077650092.1:116..2476"
                     /db_xref="GeneID:144115651"
ORIGIN      
        1 mpgwklglal sfvlvltaag rrgvrgasgd gdgedelvak wkdmeasmre lvqgalkktl
       61 pevlrmgqgd gglsapctsa figvfrrlgs ldkevmkmid ssgklapgll dgsladfgdy
      121 eeclsvavpd sssedgsllf rgqycmvksr pplppkprfv nkhfaavnlt lfpensvmre
      181 fakdagtfyt ttartglcmp svcskedvqa iaknvfrgih wdafvsdctq astahltlkd
      241 kifiavlvci slpvffstai hvianlflts pkykkshgpv lerltklsay etahkifvvr
      301 epaddetrrl qvfhglrals ivwivwvhhy ayndtsvysg akiakklvvq yntqpfnnaw
      361 lavdtfffis gffllyaink qmrsaglgka lfyivvrwwr lipmacvgif mltlirhfgd
      421 gplwneyiga eiekcnknww llllsvqnfl ahddlclvpy wyisvdtqlh iifiglgiil
      481 frnfylsata mlflvitsti vvacqtfvqn ytptalfvag dstfakevle hiyfrpythf
      541 gpfclgiita yafltkkprf elgwvcqgaa wsavflggat vlfctkewgd gdalpapwva
      601 alyaschrlv walcigwlvf acvsdrggvv nsvlsspllv plsrlsyavy vlhvplvwfr
      661 lwtirerili wflpmfynam gtlvlaffva lgasifierp lvvakdlivn rtvtprnrks
      721 lqlggprgrg ggksgmvrgt srlvpsvasi asklsggria pkkqkqqpaa vtttprfqsd
      781 sctvrl
//
LOCUS       XP_077506219             539 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome P450 3A4-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077506219
VERSION     XP_077506219.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650093.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..539
                     /product="cytochrome P450 3A4-like isoform X1"
                     /calculated_mol_wt=61437
     Region          98..523
                     /region_name="CYP3A-like"
                     /note="cytochrome P450 family 3, subfamily A and similar
                     cytochrome P450s; cd11055"
                     /db_xref="CDD:410678"
     Site            order(107,135..138,150..151,242,244..245,247,272,337,
                     340..341,345,410,412,521..522)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410678"
     Site            order(135,149..150,157,161,168,338,341..342,345,411,413,
                     474..477,480..484,488)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410678"
     CDS             1..539
                     /gene="LOC144115652"
                     /coded_by="XM_077650093.1:593..2212"
                     /db_xref="GeneID:144115652"
ORIGIN      
        1 mhrkgrrrrf gglrytedig slpsrersfa meagawgvti vlwsavlvls iawalrrrhq
       61 qrlfkrygip gpepslflgn wkqlkedrlk vmddwirqyg kvfgfyegei pkvvvsdlda
      121 ikecfvketh vfhdrvpiiv dvepvkssli glkgeewkrv rtvlspsfss akmklmwhtm
      181 sacgdtmvdv isehlcagra vvnvttlsqa fsmdvitkca lawqvecqrn aedpvmrrlq
      241 killevderl matavaipvl rkvyawifsf lsygklflli mdnlrkvisv rtaemkerpr
      301 ttvdmlqlml dakneaddga rgkaeitdrh lmancflfla agfettatal sfvlyelarh
      361 peeqervfee vtaqlsensg elgydgvqkl rrlgmvisec lrlypplvlf tarvcgrdtk
      421 lggytvpaga hvilptwhvh hrpdlwpepy rfipdrftpd keqgerrhpa ayvpfglgar
      481 ecmgrrfall evktvlaklv ssyifrvcee tddpmeltvp avtvnparki klrvarrdv
//
LOCUS       XP_077506220             509 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome P450 3A4-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077506220
VERSION     XP_077506220.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650094.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..509
                     /product="cytochrome P450 3A4-like isoform X2"
                     /calculated_mol_wt=57862
     Region          68..493
                     /region_name="CYP3A-like"
                     /note="cytochrome P450 family 3, subfamily A and similar
                     cytochrome P450s; cd11055"
                     /db_xref="CDD:410678"
     Site            order(77,105..108,120..121,212,214..215,217,242,307,
                     310..311,315,380,382,491..492)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410678"
     Site            order(105,119..120,127,131,138,308,311..312,315,381,383,
                     444..447,450..454,458)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410678"
     CDS             1..509
                     /gene="LOC144115652"
                     /coded_by="XM_077650094.1:2005..3534"
                     /db_xref="GeneID:144115652"
ORIGIN      
        1 meagawgvti vlwsavlvls iawalrrrhq qrlfkrygip gpepslflgn wkqlkedrlk
       61 vmddwirqyg kvfgfyegei pkvvvsdlda ikecfvketh vfhdrvpiiv dvepvkssli
      121 glkgeewkrv rtvlspsfss akmklmwhtm sacgdtmvdv isehlcagra vvnvttlsqa
      181 fsmdvitkca lawqvecqrn aedpvmrrlq killevderl matavaipvl rkvyawifsf
      241 lsygklflli mdnlrkvisv rtaemkerpr ttvdmlqlml dakneaddga rgkaeitdrh
      301 lmancflfla agfettatal sfvlyelarh peeqervfee vtaqlsensg elgydgvqkl
      361 rrlgmvisec lrlypplvlf tarvcgrdtk lggytvpaga hvilptwhvh hrpdlwpepy
      421 rfipdrftpd keqgerrhpa ayvpfglgar ecmgrrfall evktvlaklv ssyifrvcee
      481 tddpmeltvp avtvnparki klrvarrdv
//
LOCUS       XP_077506221             509 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome P450 3A4-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077506221
VERSION     XP_077506221.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650095.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..509
                     /product="cytochrome P450 3A4-like isoform X2"
                     /calculated_mol_wt=57862
     Region          68..493
                     /region_name="CYP3A-like"
                     /note="cytochrome P450 family 3, subfamily A and similar
                     cytochrome P450s; cd11055"
                     /db_xref="CDD:410678"
     Site            order(77,105..108,120..121,212,214..215,217,242,307,
                     310..311,315,380,382,491..492)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410678"
     Site            order(105,119..120,127,131,138,308,311..312,315,381,383,
                     444..447,450..454,458)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410678"
     CDS             1..509
                     /gene="LOC144115652"
                     /coded_by="XM_077650095.1:2074..3603"
                     /db_xref="GeneID:144115652"
ORIGIN      
        1 meagawgvti vlwsavlvls iawalrrrhq qrlfkrygip gpepslflgn wkqlkedrlk
       61 vmddwirqyg kvfgfyegei pkvvvsdlda ikecfvketh vfhdrvpiiv dvepvkssli
      121 glkgeewkrv rtvlspsfss akmklmwhtm sacgdtmvdv isehlcagra vvnvttlsqa
      181 fsmdvitkca lawqvecqrn aedpvmrrlq killevderl matavaipvl rkvyawifsf
      241 lsygklflli mdnlrkvisv rtaemkerpr ttvdmlqlml dakneaddga rgkaeitdrh
      301 lmancflfla agfettatal sfvlyelarh peeqervfee vtaqlsensg elgydgvqkl
      361 rrlgmvisec lrlypplvlf tarvcgrdtk lggytvpaga hvilptwhvh hrpdlwpepy
      421 rfipdrftpd keqgerrhpa ayvpfglgar ecmgrrfall evktvlaklv ssyifrvcee
      481 tddpmeltvp avtvnparki klrvarrdv
//
LOCUS       XP_077506222             509 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome P450 3A4-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077506222
VERSION     XP_077506222.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650096.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..509
                     /product="cytochrome P450 3A4-like isoform X2"
                     /calculated_mol_wt=57862
     Region          68..493
                     /region_name="CYP3A-like"
                     /note="cytochrome P450 family 3, subfamily A and similar
                     cytochrome P450s; cd11055"
                     /db_xref="CDD:410678"
     Site            order(77,105..108,120..121,212,214..215,217,242,307,
                     310..311,315,380,382,491..492)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410678"
     Site            order(105,119..120,127,131,138,308,311..312,315,381,383,
                     444..447,450..454,458)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410678"
     CDS             1..509
                     /gene="LOC144115652"
                     /coded_by="XM_077650096.1:1930..3459"
                     /db_xref="GeneID:144115652"
ORIGIN      
        1 meagawgvti vlwsavlvls iawalrrrhq qrlfkrygip gpepslflgn wkqlkedrlk
       61 vmddwirqyg kvfgfyegei pkvvvsdlda ikecfvketh vfhdrvpiiv dvepvkssli
      121 glkgeewkrv rtvlspsfss akmklmwhtm sacgdtmvdv isehlcagra vvnvttlsqa
      181 fsmdvitkca lawqvecqrn aedpvmrrlq killevderl matavaipvl rkvyawifsf
      241 lsygklflli mdnlrkvisv rtaemkerpr ttvdmlqlml dakneaddga rgkaeitdrh
      301 lmancflfla agfettatal sfvlyelarh peeqervfee vtaqlsensg elgydgvqkl
      361 rrlgmvisec lrlypplvlf tarvcgrdtk lggytvpaga hvilptwhvh hrpdlwpepy
      421 rfipdrftpd keqgerrhpa ayvpfglgar ecmgrrfall evktvlaklv ssyifrvcee
      481 tddpmeltvp avtvnparki klrvarrdv
//
LOCUS       XP_077506223             509 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome P450 3A4-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077506223
VERSION     XP_077506223.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650097.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..509
                     /product="cytochrome P450 3A4-like isoform X2"
                     /calculated_mol_wt=57862
     Region          68..493
                     /region_name="CYP3A-like"
                     /note="cytochrome P450 family 3, subfamily A and similar
                     cytochrome P450s; cd11055"
                     /db_xref="CDD:410678"
     Site            order(77,105..108,120..121,212,214..215,217,242,307,
                     310..311,315,380,382,491..492)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410678"
     Site            order(105,119..120,127,131,138,308,311..312,315,381,383,
                     444..447,450..454,458)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410678"
     CDS             1..509
                     /gene="LOC144115652"
                     /coded_by="XM_077650097.1:1691..3220"
                     /db_xref="GeneID:144115652"
ORIGIN      
        1 meagawgvti vlwsavlvls iawalrrrhq qrlfkrygip gpepslflgn wkqlkedrlk
       61 vmddwirqyg kvfgfyegei pkvvvsdlda ikecfvketh vfhdrvpiiv dvepvkssli
      121 glkgeewkrv rtvlspsfss akmklmwhtm sacgdtmvdv isehlcagra vvnvttlsqa
      181 fsmdvitkca lawqvecqrn aedpvmrrlq killevderl matavaipvl rkvyawifsf
      241 lsygklflli mdnlrkvisv rtaemkerpr ttvdmlqlml dakneaddga rgkaeitdrh
      301 lmancflfla agfettatal sfvlyelarh peeqervfee vtaqlsensg elgydgvqkl
      361 rrlgmvisec lrlypplvlf tarvcgrdtk lggytvpaga hvilptwhvh hrpdlwpepy
      421 rfipdrftpd keqgerrhpa ayvpfglgar ecmgrrfall evktvlaklv ssyifrvcee
      481 tddpmeltvp avtvnparki klrvarrdv
//
LOCUS       XP_077506224             509 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome P450 3A4-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077506224
VERSION     XP_077506224.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650098.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..509
                     /product="cytochrome P450 3A4-like isoform X2"
                     /calculated_mol_wt=57862
     Region          68..493
                     /region_name="CYP3A-like"
                     /note="cytochrome P450 family 3, subfamily A and similar
                     cytochrome P450s; cd11055"
                     /db_xref="CDD:410678"
     Site            order(77,105..108,120..121,212,214..215,217,242,307,
                     310..311,315,380,382,491..492)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410678"
     Site            order(105,119..120,127,131,138,308,311..312,315,381,383,
                     444..447,450..454,458)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410678"
     CDS             1..509
                     /gene="LOC144115652"
                     /coded_by="XM_077650098.1:1760..3289"
                     /db_xref="GeneID:144115652"
ORIGIN      
        1 meagawgvti vlwsavlvls iawalrrrhq qrlfkrygip gpepslflgn wkqlkedrlk
       61 vmddwirqyg kvfgfyegei pkvvvsdlda ikecfvketh vfhdrvpiiv dvepvkssli
      121 glkgeewkrv rtvlspsfss akmklmwhtm sacgdtmvdv isehlcagra vvnvttlsqa
      181 fsmdvitkca lawqvecqrn aedpvmrrlq killevderl matavaipvl rkvyawifsf
      241 lsygklflli mdnlrkvisv rtaemkerpr ttvdmlqlml dakneaddga rgkaeitdrh
      301 lmancflfla agfettatal sfvlyelarh peeqervfee vtaqlsensg elgydgvqkl
      361 rrlgmvisec lrlypplvlf tarvcgrdtk lggytvpaga hvilptwhvh hrpdlwpepy
      421 rfipdrftpd keqgerrhpa ayvpfglgar ecmgrrfall evktvlaklv ssyifrvcee
      481 tddpmeltvp avtvnparki klrvarrdv
//
LOCUS       XP_077506225             136 aa            linear   INV 25-NOV-2025
DEFINITION  prolyl 4-hydroxylase subunit alpha-2-like [Amblyomma americanum].
ACCESSION   XP_077506225
VERSION     XP_077506225.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650099.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..136
                     /product="prolyl 4-hydroxylase subunit alpha-2-like"
                     /calculated_mol_wt=14300
     Region          <19..128
                     /region_name="P4Hc"
                     /note="Prolyl 4-hydroxylase alpha subunit homologues;
                     smart00702"
                     /db_xref="CDD:214780"
     CDS             1..136
                     /gene="LOC144115653"
                     /coded_by="XM_077650099.1:863..1273"
                     /db_xref="GeneID:144115653"
ORIGIN      
        1 mpyvhkvshg ikkvaarqgv swlsdedhpl lpeltarvaa atglavdsae pfqvvnyglg
       61 gyyalhldai efdgagtlgv erisgnrlat mlvflsdvps ggatafaepa laakpragda
      121 lfwfdlrpya alhigr
//
LOCUS       XP_077506226             800 aa            linear   INV 25-NOV-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Amblyomma americanum].
ACCESSION   XP_077506226
VERSION     XP_077506226.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650100.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..800
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=88438
     Region          136..270
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          364..773
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..800
                     /gene="LOC144115656"
                     /coded_by="XM_077650100.1:344..2746"
                     /db_xref="GeneID:144115656"
ORIGIN      
        1 mrclkhgrrn rgdtsldvaa afpvlvflfa alagfngvav cdaspppptt aqvaaatpts
       61 agsdaevvds gavtepampr dvarskdgdn esapydvqad wellesklss vtngalnqll
      121 peltalvagl dlntdcflsl lrvigglrkl nlesvrmlds ssklpagile gtvtdlgdyd
      181 tcldlsfpsr fdarledfrg qycgvairfp lpvkpdvirh gavivnpagf pdtsvvkyla
      241 rksytlyhtf irlglclpsh cteedvqkli spiktylhln atvlncqvrq dnhsmnkdqv
      301 aaiaiigffv viilvatvvd iiyrlyenwr chaymkkpmv qslvdmsllr ateklltvkt
      361 pedrhnknlq avhgirtfnv fwivvghtya faeqttyrsg nniltmagnv lfqpvinsfl
      421 cvdsffflsg flltfnqckk pvkttpvvdf lgkllarywr lvpvaavcmt vlfllpelas
      481 gpiwnekvar aignchrsww svllnihnfy syeescqphy wyisadlqif pvvlatsilm
      541 grnvklgvii sssilvasvv vvgaltying yppailllgk dvlyskells eiyfrpyvhv
      601 gpylmgslva clylkrqrlr isprlecagw laclaagayv nlvtvawedg rypsplwssa
      661 yaalhrvmwa aclgwvvyac avgrggilnt ilswkplvpv srisftiyvv hiyviylklw
      721 tirerlspdh fhvfmsaisn fvvavilgfv asvlfekpiv wlwdnvtgsh kpprlaphvt
      781 kqppqeaasp aangikkepa
//
LOCUS       XP_077506227             296 aa            linear   INV 25-NOV-2025
DEFINITION  hatching enzyme 1.2-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077506227
VERSION     XP_077506227.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650101.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..296
                     /product="hatching enzyme 1.2-like isoform X1"
                     /calculated_mol_wt=33802
     Region          74..>200
                     /region_name="ZnMc"
                     /note="Zinc-dependent metalloprotease. This super-family
                     of metalloproteases contains two major branches, the
                     astacin-like proteases and the adamalysin/reprolysin-like
                     proteases. Both branches have wide phylogenetic
                     distribution, and contain sub-families, which...; cl00064"
                     /db_xref="CDD:469599"
     Site            order(166..167,170,176)
                     /site_type="active"
                     /db_xref="CDD:238124"
     CDS             1..296
                     /gene="LOC144115657"
                     /coded_by="XM_077650101.1:98..988"
                     /db_xref="GeneID:144115657"
ORIGIN      
        1 mavlraccwl cvavmlisvp rlvagrpvra rsrvfqynpn ntdliegdii ppkqskqrft
       61 tyavvkdral twpdgtvfyk ydpdsfppgp eeeeesedse ryiivqamql iqektcvkfe
      121 phtdqknfvt fahrpgrcas hvgmqgeeqv valdlsycpg lgqvahellh vlgffhehsr
      181 pdrdeyvdvl wdnikpghqn rlqahifcqq hwfsqrwetr sgaacetkar eplpssaply
      241 vlfanflace pplrrkgvvq slwrlrarrm vpacrlarpp lpdwaarrar piafsg
//
LOCUS       XP_077506228             279 aa            linear   INV 25-NOV-2025
DEFINITION  zinc metalloproteinase nas-14-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506228
VERSION     XP_077506228.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650102.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..279
                     /product="zinc metalloproteinase nas-14-like isoform X2"
                     /calculated_mol_wt=31619
     Region          74..272
                     /region_name="ZnMc_astacin_like"
                     /note="Zinc-dependent metalloprotease, astacin_like
                     subfamily or peptidase family M12A, a group of
                     zinc-dependent proteolytic enzymes with a HExxH
                     zinc-binding site/active site. Members of this family may
                     have an amino terminal propeptide, which is cleaved to...;
                     cd04280"
                     /db_xref="CDD:239807"
     Site            order(166..167,170,176,225)
                     /site_type="active"
                     /db_xref="CDD:239807"
     CDS             1..279
                     /gene="LOC144115657"
                     /coded_by="XM_077650102.1:88..927"
                     /db_xref="GeneID:144115657"
ORIGIN      
        1 mavlraccwl cvavmlisvp rlvagrpvra rsrvfqynpn ntdliegdii ppkqskqrft
       61 tyavvkdral twpdgtvfyk ydpdsfppgp eeeeesedse ryiivqamql iqektcvkfe
      121 phtdqknfvt fahrpgrcas hvgmqgeeqv valdlsycpg lgqvahellh vlgffhehsr
      181 pdrdeyvdvl wdnikpdanq sfwlkpetea dtlgepydye svmhypfdaf avkeaeptll
      241 pksvnvkret lgkayaegfl tdtditqinv lytcgkqps
//
LOCUS       XP_077506230             212 aa            linear   INV 25-NOV-2025
DEFINITION  nematocyst expressed protein 6-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077506230
VERSION     XP_077506230.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650104.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..212
                     /product="nematocyst expressed protein 6-like isoform X3"
                     /calculated_mol_wt=24044
     Region          74..>197
                     /region_name="ZnMc"
                     /note="Zinc-dependent metalloprotease. This super-family
                     of metalloproteases contains two major branches, the
                     astacin-like proteases and the adamalysin/reprolysin-like
                     proteases. Both branches have wide phylogenetic
                     distribution, and contain sub-families, which...; cl00064"
                     /db_xref="CDD:469599"
     Site            order(166..167,170,176)
                     /site_type="active"
                     /db_xref="CDD:238124"
     CDS             1..212
                     /gene="LOC144115657"
                     /coded_by="XM_077650104.1:93..731"
                     /db_xref="GeneID:144115657"
ORIGIN      
        1 mavlraccwl cvavmlisvp rlvagrpvra rsrvfqynpn ntdliegdii ppkqskqrft
       61 tyavvkdral twpdgtvfyk ydpdsfppgp eeeeesedse ryiivqamql iqektcvkfe
      121 phtdqknfvt fahrpgrcas hvgmqgeeqv valdlsycpg lgqvahellh vlgffhehsr
      181 pdrdeyvdvl wdnikpgepr aivvaehegl hf
//
LOCUS       XP_077506231             209 aa            linear   INV 25-NOV-2025
DEFINITION  nematocyst expressed protein 6-like isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077506231
VERSION     XP_077506231.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650105.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..209
                     /product="nematocyst expressed protein 6-like isoform X4"
                     /calculated_mol_wt=23591
     Region          74..>198
                     /region_name="ZnMc"
                     /note="Zinc-dependent metalloprotease. This super-family
                     of metalloproteases contains two major branches, the
                     astacin-like proteases and the adamalysin/reprolysin-like
                     proteases. Both branches have wide phylogenetic
                     distribution, and contain sub-families, which...; cl00064"
                     /db_xref="CDD:469599"
     Site            order(166..167,170,176)
                     /site_type="active"
                     /db_xref="CDD:238124"
     CDS             1..209
                     /gene="LOC144115657"
                     /coded_by="XM_077650105.1:93..722"
                     /db_xref="GeneID:144115657"
ORIGIN      
        1 mavlraccwl cvavmlisvp rlvagrpvra rsrvfqynpn ntdliegdii ppkqskqrft
       61 tyavvkdral twpdgtvfyk ydpdsfppgp eeeeesedse ryiivqamql iqektcvkfe
      121 phtdqknfvt fahrpgrcas hvgmqgeeqv valdlsycpg lgqvahellh vlgffhehsr
      181 pdrdeyvdvl wdnikpgfsg matlrptav
//
LOCUS       XP_077506232             915 aa            linear   INV 25-NOV-2025
DEFINITION  nuclear factor NF-kappa-B family member relish isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077506232
VERSION     XP_077506232.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650106.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..915
                     /product="nuclear factor NF-kappa-B family member relish
                     isoform X1"
                     /calculated_mol_wt=100809
     Region          106..279
                     /region_name="RHD-n"
                     /note="N-terminal sub-domain of the Rel homology domain
                     (RHD); cl08275"
                     /db_xref="CDD:447596"
     Site            order(117,119..120,122..123,125..128,206,208..209,279)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143640"
     Region          284..386
                     /region_name="RHD_dimer"
                     /note="Rel homology dimerization domain; pfam16179"
                     /db_xref="CDD:465045"
     Region          571..663
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          593..630
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <626..795
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(632,634,638..639,642..644,646..647,651,654,663,665,
                     667,671..672,674..676,678,681,685,688,699,701,703,
                     707..708,711..713,715..716,720,723)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          632..663
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          665..699
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          701..730
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          735..767
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          769..795
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..915
                     /gene="Rel"
                     /coded_by="XM_077650106.1:122..2869"
                     /db_xref="GeneID:144115659"
ORIGIN      
        1 msllvdygwi tsslcgtaaa amddwpyyed dpygaplgnv aypvsgeeaa aasssssaeq
       61 eqlrsfqdll lrsfqhappe aheigmpict dfeeqqnlrm gggarliile qpmketryry
      121 ksesgshgpl igesstqqrk tfptvkleny npelphrika slvtaedtar phvhritmrg
      181 rdeedccyvt vredgraifp smsivfqqkk tvadilyrrk iesrqpsqee arqlqteakk
      241 eaaelnlnlv ricftaecce rgvwkplcva ysnpvanska gklritkanr ksgsclggde
      301 vwilcekink kdilikffee deetrertwe apatfqesdv hyqvaivfkt payrdtnlqr
      361 qvpvkfqlir ksdndcsepf eftylpcapt dedvlvhkrp kmsqqtmdes shlsgmmgpp
      421 htnssggsss spslflnepp fsmetsypaa gedvgdepll sddlmavlhe nlkdfycllw
      481 egsanpvlsq gmaadsrkle esmrklslsg dedgagynpc kmdskmplye efsspshsrd
      541 daykrrwcna agrqlgdamy gllcwltkhl vkncstdili klvsdltslc dvdgnnalhi
      601 avghpscfle tlltvvdstk khlecvskqn nrgqtplhls tlqgrsdhss lllvhgadvt
      661 sldrwgrtvl hcailgcmsk eglkrllsyr pkllvnaada egktavqiav eqqnevalrv
      721 lcsvgadvna pvdrtgdral htavlkdfvd gvrillaqdt idttainshg lraldmaldl
      781 draqiihmll vhaenscsmg qqqmdveggd gnndwlqgde ayasssslvs dgggaegvtp
      841 nglresimaa ldseenaarv ldclvgggda ivqkvvaflk ggglgraiyr kylmdfsdea
      901 lqrlftevlc iplda
//
LOCUS       XP_077506233             889 aa            linear   INV 25-NOV-2025
DEFINITION  nuclear factor NF-kappa-B family member relish isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077506233
VERSION     XP_077506233.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650107.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..889
                     /product="nuclear factor NF-kappa-B family member relish
                     isoform X2"
                     /calculated_mol_wt=97986
     Region          80..253
                     /region_name="RHD-n"
                     /note="N-terminal sub-domain of the Rel homology domain
                     (RHD); cl08275"
                     /db_xref="CDD:447596"
     Site            order(91,93..94,96..97,99..102,180,182..183,253)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143640"
     Region          258..360
                     /region_name="RHD_dimer"
                     /note="Rel homology dimerization domain; pfam16179"
                     /db_xref="CDD:465045"
     Region          545..637
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          567..604
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <600..769
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(606,608,612..613,616..618,620..621,625,628,637,639,
                     641,645..646,648..650,652,655,659,662,673,675,677,
                     681..682,685..687,689..690,694,697)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          606..637
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          639..673
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          675..704
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          709..741
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          743..769
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..889
                     /gene="Rel"
                     /coded_by="XM_077650107.1:68..2737"
                     /db_xref="GeneID:144115659"
ORIGIN      
        1 meeddpygap lgnvaypvsg eeaaaassss saeqeqlrsf qdlllrsfqh appeaheigm
       61 pictdfeeqq nlrmgggarl iileqpmket ryryksesgs hgpligesst qqrktfptvk
      121 lenynpelph rikaslvtae dtarphvhri tmrgrdeedc cyvtvredgr aifpsmsivf
      181 qqkktvadil yrrkiesrqp sqeearqlqt eakkeaaeln lnlvricfta eccergvwkp
      241 lcvaysnpva nskagklrit kanrksgscl ggdevwilce kinkkdilik ffeedeetre
      301 rtweapatfq esdvhyqvai vfktpayrdt nlqrqvpvkf qlirksdndc sepfeftylp
      361 captdedvlv hkrpkmsqqt mdesshlsgm mgpphtnssg gsssspslfl neppfsmets
      421 ypaagedvgd epllsddlma vlhenlkdfy cllwegsanp vlsqgmaads rkleesmrkl
      481 slsgdedgag ynpckmdskm plyeefssps hsrddaykrr wcnaagrqlg damygllcwl
      541 tkhlvkncst diliklvsdl tslcdvdgnn alhiavghps cfletlltvv dstkkhlecv
      601 skqnnrgqtp lhlstlqgrs dhsslllvhg advtsldrwg rtvlhcailg cmskeglkrl
      661 lsyrpkllvn aadaegktav qiaveqqnev alrvlcsvga dvnapvdrtg dralhtavlk
      721 dfvdgvrill aqdtidttai nshglraldm aldldraqii hmllvhaens csmgqqqmdv
      781 eggdgnndwl qgdeayasss slvsdgggae gvtpnglres imaaldseen aarvldclvg
      841 ggdaivqkvv aflkggglgr aiyrkylmdf sdealqrlft evlciplda
//
LOCUS       XP_077506234             869 aa            linear   INV 25-NOV-2025
DEFINITION  nuclear factor NF-kappa-B family member relish isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077506234
VERSION     XP_077506234.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650108.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..869
                     /product="nuclear factor NF-kappa-B family member relish
                     isoform X3"
                     /calculated_mol_wt=95748
     Region          106..279
                     /region_name="RHD-n"
                     /note="N-terminal sub-domain of the Rel homology domain
                     (RHD); cl08275"
                     /db_xref="CDD:447596"
     Site            order(117,119..120,122..123,125..128,206,208..209,279)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143640"
     Region          284..386
                     /region_name="RHD_dimer"
                     /note="Rel homology dimerization domain; pfam16179"
                     /db_xref="CDD:465045"
     Region          571..663
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          593..630
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <626..777
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(632,634,638..639,642..644,646..647,651,654,663,665,
                     667,671..672,674..676,678,681,685,688,699,701,703,
                     707..708,711..713,715..716,720,723)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          632..663
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          665..699
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          701..730
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..869
                     /gene="Rel"
                     /coded_by="XM_077650108.1:119..2728"
                     /db_xref="GeneID:144115659"
ORIGIN      
        1 msllvdygwi tsslcgtaaa amddwpyyed dpygaplgnv aypvsgeeaa aasssssaeq
       61 eqlrsfqdll lrsfqhappe aheigmpict dfeeqqnlrm gggarliile qpmketryry
      121 ksesgshgpl igesstqqrk tfptvkleny npelphrika slvtaedtar phvhritmrg
      181 rdeedccyvt vredgraifp smsivfqqkk tvadilyrrk iesrqpsqee arqlqteakk
      241 eaaelnlnlv ricftaecce rgvwkplcva ysnpvanska gklritkanr ksgsclggde
      301 vwilcekink kdilikffee deetrertwe apatfqesdv hyqvaivfkt payrdtnlqr
      361 qvpvkfqlir ksdndcsepf eftylpcapt dedvlvhkrp kmsqqtmdes shlsgmmgpp
      421 htnssggsss spslflnepp fsmetsypaa gedvgdepll sddlmavlhe nlkdfycllw
      481 egsanpvlsq gmaadsrkle esmrklslsg dedgagynpc kmdskmplye efsspshsrd
      541 daykrrwcna agrqlgdamy gllcwltkhl vkncstdili klvsdltslc dvdgnnalhi
      601 avghpscfle tlltvvdstk khlecvskqn nrgqtplhls tlqgrsdhss lllvhgadvt
      661 sldrwgrtvl hcailgcmsk eglkrllsyr pkllvnaada egktavqiav eqqnevalrv
      721 lcsvgadvna pvdrtgdral htavlkdfvd gvrillaqdv eggdgnndwl qgdeayasss
      781 slvsdgggae gvtpnglres imaaldseen aarvldclvg ggdaivqkvv aflkggglgr
      841 aiyrkylmdf sdealqrlft evlciplda
//
LOCUS       XP_077506235             830 aa            linear   INV 25-NOV-2025
DEFINITION  nuclear factor NF-kappa-B family member relish isoform X4
            [Amblyomma americanum].
ACCESSION   XP_077506235
VERSION     XP_077506235.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650109.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..830
                     /product="nuclear factor NF-kappa-B family member relish
                     isoform X4"
                     /calculated_mol_wt=91715
     Region          21..194
                     /region_name="RHD-n"
                     /note="N-terminal sub-domain of the Rel homology domain
                     (RHD); cl08275"
                     /db_xref="CDD:447596"
     Site            order(32,34..35,37..38,40..43,121,123..124,194)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143640"
     Region          199..301
                     /region_name="RHD_dimer"
                     /note="Rel homology dimerization domain; pfam16179"
                     /db_xref="CDD:465045"
     Region          486..578
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          508..545
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <541..710
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(547,549,553..554,557..559,561..562,566,569,578,580,
                     582,586..587,589..591,593,596,600,603,614,616,618,
                     622..623,626..628,630..631,635,638)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          547..578
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          580..614
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          616..645
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          650..682
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          684..710
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..830
                     /gene="Rel"
                     /coded_by="XM_077650109.1:456..2948"
                     /db_xref="GeneID:144115659"
ORIGIN      
        1 mpictdfeeq qnlrmgggar liileqpmke tryryksesg shgpligess tqqrktfptv
       61 klenynpelp hrikaslvta edtarphvhr itmrgrdeed ccyvtvredg raifpsmsiv
      121 fqqkktvadi lyrrkiesrq psqeearqlq teakkeaael nlnlvricft aeccergvwk
      181 plcvaysnpv anskagklri tkanrksgsc lggdevwilc ekinkkdili kffeedeetr
      241 ertweapatf qesdvhyqva ivfktpayrd tnlqrqvpvk fqlirksdnd csepfeftyl
      301 pcaptdedvl vhkrpkmsqq tmdesshlsg mmgpphtnss ggsssspslf lneppfsmet
      361 sypaagedvg depllsddlm avlhenlkdf ycllwegsan pvlsqgmaad srkleesmrk
      421 lslsgdedga gynpckmdsk mplyeefssp shsrddaykr rwcnaagrql gdamygllcw
      481 ltkhlvkncs tdiliklvsd ltslcdvdgn nalhiavghp scfletlltv vdstkkhlec
      541 vskqnnrgqt plhlstlqgr sdhsslllvh gadvtsldrw grtvlhcail gcmskeglkr
      601 llsyrpkllv naadaegkta vqiaveqqne valrvlcsvg advnapvdrt gdralhtavl
      661 kdfvdgvril laqdtidtta inshglrald maldldraqi ihmllvhaen scsmgqqqmd
      721 veggdgnndw lqgdeayass sslvsdggga egvtpnglre simaaldsee naarvldclv
      781 gggdaivqkv vaflkggglg raiyrkylmd fsdealqrlf tevlciplda
//
LOCUS       XP_077506236             830 aa            linear   INV 25-NOV-2025
DEFINITION  nuclear factor NF-kappa-B family member relish isoform X4
            [Amblyomma americanum].
ACCESSION   XP_077506236
VERSION     XP_077506236.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650110.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..830
                     /product="nuclear factor NF-kappa-B family member relish
                     isoform X4"
                     /calculated_mol_wt=91715
     Region          21..194
                     /region_name="RHD-n"
                     /note="N-terminal sub-domain of the Rel homology domain
                     (RHD); cl08275"
                     /db_xref="CDD:447596"
     Site            order(32,34..35,37..38,40..43,121,123..124,194)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143640"
     Region          199..301
                     /region_name="RHD_dimer"
                     /note="Rel homology dimerization domain; pfam16179"
                     /db_xref="CDD:465045"
     Region          486..578
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          508..545
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <541..710
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(547,549,553..554,557..559,561..562,566,569,578,580,
                     582,586..587,589..591,593,596,600,603,614,616,618,
                     622..623,626..628,630..631,635,638)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          547..578
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          580..614
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          616..645
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          650..682
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          684..710
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..830
                     /gene="Rel"
                     /coded_by="XM_077650110.1:208..2700"
                     /db_xref="GeneID:144115659"
ORIGIN      
        1 mpictdfeeq qnlrmgggar liileqpmke tryryksesg shgpligess tqqrktfptv
       61 klenynpelp hrikaslvta edtarphvhr itmrgrdeed ccyvtvredg raifpsmsiv
      121 fqqkktvadi lyrrkiesrq psqeearqlq teakkeaael nlnlvricft aeccergvwk
      181 plcvaysnpv anskagklri tkanrksgsc lggdevwilc ekinkkdili kffeedeetr
      241 ertweapatf qesdvhyqva ivfktpayrd tnlqrqvpvk fqlirksdnd csepfeftyl
      301 pcaptdedvl vhkrpkmsqq tmdesshlsg mmgpphtnss ggsssspslf lneppfsmet
      361 sypaagedvg depllsddlm avlhenlkdf ycllwegsan pvlsqgmaad srkleesmrk
      421 lslsgdedga gynpckmdsk mplyeefssp shsrddaykr rwcnaagrql gdamygllcw
      481 ltkhlvkncs tdiliklvsd ltslcdvdgn nalhiavghp scfletlltv vdstkkhlec
      541 vskqnnrgqt plhlstlqgr sdhsslllvh gadvtsldrw grtvlhcail gcmskeglkr
      601 llsyrpkllv naadaegkta vqiaveqqne valrvlcsvg advnapvdrt gdralhtavl
      661 kdfvdgvril laqdtidtta inshglrald maldldraqi ihmllvhaen scsmgqqqmd
      721 veggdgnndw lqgdeayass sslvsdggga egvtpnglre simaaldsee naarvldclv
      781 gggdaivqkv vaflkggglg raiyrkylmd fsdealqrlf tevlciplda
//
LOCUS       XP_077506237             784 aa            linear   INV 25-NOV-2025
DEFINITION  nuclear factor NF-kappa-B family member relish isoform X5
            [Amblyomma americanum].
ACCESSION   XP_077506237
VERSION     XP_077506237.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650111.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..784
                     /product="nuclear factor NF-kappa-B family member relish
                     isoform X5"
                     /calculated_mol_wt=86654
     Region          21..194
                     /region_name="RHD-n"
                     /note="N-terminal sub-domain of the Rel homology domain
                     (RHD); cl08275"
                     /db_xref="CDD:447596"
     Site            order(32,34..35,37..38,40..43,121,123..124,194)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143640"
     Region          199..301
                     /region_name="RHD_dimer"
                     /note="Rel homology dimerization domain; pfam16179"
                     /db_xref="CDD:465045"
     Region          486..578
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          508..545
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <541..692
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(547,549,553..554,557..559,561..562,566,569,578,580,
                     582,586..587,589..591,593,596,600,603,614,616,618,
                     622..623,626..628,630..631,635,638)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          547..578
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          580..614
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          616..645
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..784
                     /gene="Rel"
                     /coded_by="XM_077650111.1:456..2810"
                     /db_xref="GeneID:144115659"
ORIGIN      
        1 mpictdfeeq qnlrmgggar liileqpmke tryryksesg shgpligess tqqrktfptv
       61 klenynpelp hrikaslvta edtarphvhr itmrgrdeed ccyvtvredg raifpsmsiv
      121 fqqkktvadi lyrrkiesrq psqeearqlq teakkeaael nlnlvricft aeccergvwk
      181 plcvaysnpv anskagklri tkanrksgsc lggdevwilc ekinkkdili kffeedeetr
      241 ertweapatf qesdvhyqva ivfktpayrd tnlqrqvpvk fqlirksdnd csepfeftyl
      301 pcaptdedvl vhkrpkmsqq tmdesshlsg mmgpphtnss ggsssspslf lneppfsmet
      361 sypaagedvg depllsddlm avlhenlkdf ycllwegsan pvlsqgmaad srkleesmrk
      421 lslsgdedga gynpckmdsk mplyeefssp shsrddaykr rwcnaagrql gdamygllcw
      481 ltkhlvkncs tdiliklvsd ltslcdvdgn nalhiavghp scfletlltv vdstkkhlec
      541 vskqnnrgqt plhlstlqgr sdhsslllvh gadvtsldrw grtvlhcail gcmskeglkr
      601 llsyrpkllv naadaegkta vqiaveqqne valrvlcsvg advnapvdrt gdralhtavl
      661 kdfvdgvril laqdveggdg nndwlqgdea yasssslvsd gggaegvtpn glresimaal
      721 dseenaarvl dclvgggdai vqkvvaflkg gglgraiyrk ylmdfsdeal qrlftevlci
      781 plda
//
LOCUS       XP_077506238            1617 aa            linear   INV 25-NOV-2025
DEFINITION  laminin subunit gamma-1 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077506238
VERSION     XP_077506238.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650112.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1617
                     /product="laminin subunit gamma-1 isoform X1"
                     /calculated_mol_wt=178860
     Region          48..282
                     /region_name="Laminin_N"
                     /note="Laminin N-terminal (Domain VI); pfam00055"
                     /db_xref="CDD:459653"
     Region          283..340
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(284,286,293,305,307,316)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          342..399
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(342,344,351,367,370,379)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          397..441
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domai;
                     smart00180"
                     /db_xref="CDD:214543"
     Site            order(397,399,409,415,417,426)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          444..494
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(444,446,458,465,467,476)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          560..692
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          727..775
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(728,730,737,744,746,755)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          776..823
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(777,779,789,795,798,807)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          831..881
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(831,833,845,852,855,864)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          885..934
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(886,888,899,906,908,917)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          936..981
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domai;
                     smart00180"
                     /db_xref="CDD:214543"
     Site            order(936,938,948,955,957,966)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          983..1030
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(984,986,996,1002,1004,1013)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1071..>1601
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..1617
                     /gene="LanB2"
                     /coded_by="XM_077650112.1:118..4971"
                     /db_xref="GeneID:144115661"
ORIGIN      
        1 marsppagak algclllvtl agvsssqqqg rpsvrgscyd rykraqrcip efvnaafnvr
       61 veatntcglr eptkycpqtd vpgatktcev cdasvphqah padyltdfnn ndnttwwqse
      121 tmlegiqypn qvnltlnlgr afdityvrlk fhsmrpesfa iykrtrpgsp wvpyqyysst
      181 cqdtyglrps lhvtpdnetr alctgefsgl splsggsvaf stlegrpsaf hfehsrllqe
      241 wvtatairiv lnrlntfgde ifgdkqvlrs yyyaiadlav ggrcqcngha macdpgpggd
      301 rqllcrcehn tagdncercm dlyndqawgr atstnahecq pcncngrsrk cyfdqelfer
      361 tghgghcvnc sdntdgpnce rcrenyyqns dgrcmpcncd pvgsrmlqcn skgqcpckpg
      421 vegqrcdrca pnfydfsedg crlcgcsqag svdsppscnp etgvcrcktn vegrqcdrck
      481 pgffnlqrdn efgctpcfcf ghsavcqsap gysrysflst fardkehwka adkqghgvnt
      541 rheyaahhla vvapadeavy flapdrylgd qrasynqnlt ftlrigeegp kssvedivle
      601 gahgsvsqhi fgqgngmpsi rnqeyrfrlh edprygwnpt lssrefisil snltairira
      661 tyvargtgfi dniqlqsaqp spygseaatw vemctcpegy vgqfcescap gfrhdpprgg
      721 kfsrcvpcnc nghalicepd tghcicqhnt agefcercap rfygnalagt pddcqpcpcp
      781 dqgacavlpd esvvclecpq gysgrrcdlc fdghygdpqg nfgpkvpckk cecndnidpn
      841 avancntttg qclkcvyntt gfncerclpg hygdalavpk gdckpcdchr lgslegsecd
      901 prngqcacka nvagrqcaqc regywnlqsg dgceacncnl vgsynrtcdq rtghchcrpg
      961 vtgkqcdqcl pyhygfspdg cvpcdcdsqg svslqcapdg qcecrpnveg rrcerckenk
     1021 fnkeagcvdc ptcynlvqda vnehrdkvae larlldniek npqivksvdf eekleqvmdi
     1081 vnglwkdakq asgtgeslae rldellrrik kvqdiagkig leialaeniv egaehnmtsa
     1141 eavverikst ltqaqkylee eaqnalkral ersdrygqqs emmsqiarda rqladqhegd
     1201 ageiahmaad alnttqeara iaegalstqd kteseiyqlk nrmreimdlm lrtkkmaeea
     1261 keevrkaydd alrvlsdats vqmpeidtde lsrkaqeiid eagriqkead ellkkhaavl
     1321 ervgpqreea edllaeaqrq qqitdrllae tdaafakake aaaegerile eaqntlnvlk
     1381 efdenvkask dkaraalqrv peiqalieea enktrdarga lkdaeadana asdtakeaqr
     1441 iaeqasqdai nvrdeadnak tsarqlgdea eelarkvedt dermrgfeeq argdmelake
     1501 alekaneart gakqasdkva aaaatvneil kalanvdnvd prrldqleqr lmeaegeaer
     1561 arlderleel rgarqqqqgw mhdyeaqieq lkrdvanvkd isdslpdrcf rriklep
//
LOCUS       XP_077506239            1555 aa            linear   INV 25-NOV-2025
DEFINITION  laminin subunit gamma-1 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077506239
VERSION     XP_077506239.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650113.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1555
                     /product="laminin subunit gamma-1 isoform X2"
                     /calculated_mol_wt=172329
     Region          48..282
                     /region_name="Laminin_N"
                     /note="Laminin N-terminal (Domain VI); pfam00055"
                     /db_xref="CDD:459653"
     Region          283..340
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(284,286,293,305,307,316)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          342..399
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(342,344,351,367,370,379)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          397..441
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domai;
                     smart00180"
                     /db_xref="CDD:214543"
     Site            order(397,399,409,415,417,426)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          444..494
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(444,446,458,465,467,476)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          560..692
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          727..761
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Region          769..819
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(769,771,783,790,793,802)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          823..872
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(824,826,837,844,846,855)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          874..919
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domai;
                     smart00180"
                     /db_xref="CDD:214543"
     Site            order(874,876,886,893,895,904)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          921..968
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(922,924,934,940,942,951)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1009..>1539
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..1555
                     /gene="LanB2"
                     /coded_by="XM_077650113.1:118..4785"
                     /db_xref="GeneID:144115661"
ORIGIN      
        1 marsppagak algclllvtl agvsssqqqg rpsvrgscyd rykraqrcip efvnaafnvr
       61 veatntcglr eptkycpqtd vpgatktcev cdasvphqah padyltdfnn ndnttwwqse
      121 tmlegiqypn qvnltlnlgr afdityvrlk fhsmrpesfa iykrtrpgsp wvpyqyysst
      181 cqdtyglrps lhvtpdnetr alctgefsgl splsggsvaf stlegrpsaf hfehsrllqe
      241 wvtatairiv lnrlntfgde ifgdkqvlrs yyyaiadlav ggrcqcngha macdpgpggd
      301 rqllcrcehn tagdncercm dlyndqawgr atstnahecq pcncngrsrk cyfdqelfer
      361 tghgghcvnc sdntdgpnce rcrenyyqns dgrcmpcncd pvgsrmlqcn skgqcpckpg
      421 vegqrcdrca pnfydfsedg crlcgcsqag svdsppscnp etgvcrcktn vegrqcdrck
      481 pgffnlqrdn efgctpcfcf ghsavcqsap gysrysflst fardkehwka adkqghgvnt
      541 rheyaahhla vvapadeavy flapdrylgd qrasynqnlt ftlrigeegp kssvedivle
      601 gahgsvsqhi fgqgngmpsi rnqeyrfrlh edprygwnpt lssrefisil snltairira
      661 tyvargtgfi dniqlqsaqp spygseaatw vemctcpegy vgqfcescap gfrhdpprgg
      721 kfsrcvpcnc nghalicepd tgrrcdlcfd ghygdpqgnf gpkvpckkce cndnidpnav
      781 ancntttgqc lkcvynttgf ncerclpghy gdalavpkgd ckpcdchrlg slegsecdpr
      841 ngqcackanv agrqcaqcre gywnlqsgdg ceacncnlvg synrtcdqrt ghchcrpgvt
      901 gkqcdqclpy hygfspdgcv pcdcdsqgsv slqcapdgqc ecrpnvegrr cerckenkfn
      961 keagcvdcpt cynlvqdavn ehrdkvaela rlldnieknp qivksvdfee kleqvmdivn
     1021 glwkdakqas gtgeslaerl dellrrikkv qdiagkigle ialaeniveg aehnmtsaea
     1081 vverikstlt qaqkyleeea qnalkraler sdrygqqsem msqiardarq ladqhegdag
     1141 eiahmaadal nttqearaia egalstqdkt eseiyqlknr mreimdlmlr tkkmaeeake
     1201 evrkayddal rvlsdatsvq mpeidtdels rkaqeiidea griqkeadel lkkhaavler
     1261 vgpqreeaed llaeaqrqqq itdrllaetd aafakakeaa aegerileea qntlnvlkef
     1321 denvkaskdk araalqrvpe iqalieeaen ktrdargalk daeadanaas dtakeaqria
     1381 eqasqdainv rdeadnakts arqlgdeaee larkvedtde rmrgfeeqar gdmelakeal
     1441 ekaneartga kqasdkvaaa aatvneilka lanvdnvdpr rldqleqrlm eaegeaerar
     1501 lderleelrg arqqqqgwmh dyeaqieqlk rdvanvkdis dslpdrcfrr iklep
//
LOCUS       XP_077506240             182 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115662 [Amblyomma americanum].
ACCESSION   XP_077506240
VERSION     XP_077506240.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650114.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..182
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..182
                     /product="uncharacterized protein LOC144115662"
                     /calculated_mol_wt=19501
     CDS             1..182
                     /gene="LOC144115662"
                     /coded_by="XM_077650114.1:4..552"
                     /db_xref="GeneID:144115662"
ORIGIN      
        1 mssggeagsg evsagerdsa tsaagrlssh rrrataattt aqewgrprga eaglaplrsg
       61 lptresytra drerfrgpvl lrsmpsprsr vvrpgsqlaa rrhsapsgrr shegdakgqs
      121 frveqlagfa plkvrasrap erkdvfsfld hpdhvpsife lkndsrvgar slriscspsd
      181 ec
//
LOCUS       XP_077506241             547 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115663 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506241
VERSION     XP_077506241.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650115.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..547
                     /product="uncharacterized protein LOC144115663 isoform X1"
                     /calculated_mol_wt=60476
     Region          182..>233
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     Region          294..374
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(299,301,303,329..331,367)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133151"
     Site            299..301
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133151"
     Site            299
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133151"
     Site            order(329..332,335..338)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133151"
     Region          <439..500
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     CDS             1..547
                     /gene="LOC144115663"
                     /coded_by="XM_077650115.1:1..1644"
                     /db_xref="GeneID:144115663"
ORIGIN      
        1 mtslqlptfd dtsdkwktyl iraeayfean aitdskqqra llvaalstqt vqvlagriap
       61 rtpnsltyde avaalnsyyd pkrheiaesy kfftrcqqeg eavraflvei rriadgcnfg
      121 saldrmlrdr ivcgvrstnl rkqllakkdl tleeaealai aaetaendan disgapptvl
      181 rmqashrsss rdtsgaaqec grcgstkhdd nacrwanarc frcrqrghla kkcyrartde
      241 naarrtasvk tltvetasrn enkdeahiwt lisgrkngle pplrrtfrwg eidldmevdt
      301 gspvcvisrn vydrhraqwp rlksphvkls cyagqlpvlg elelpvqfkn vsvtcplvvl
      361 dcsgpslcgr dliaalgktg vtmeeltapd sttgntpsdh mvnrllaeyk dvfsidqgli
      421 kgppaslklk etatpkfcrq ftlvtdhqpl lgllrpdrqt ppmaaariqr walflggyqy
      481 klqyvpgkql ltadalsrlp aptmepgtdg eppeyvlsle sleeggppke keghlprcfs
      541 aiscvpd
//
LOCUS       XP_077506243             516 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115663 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506243
VERSION     XP_077506243.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650117.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..516
                     /product="uncharacterized protein LOC144115663 isoform X2"
                     /calculated_mol_wt=57333
     Region          <204..>233
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     Region          294..374
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(299,301,303,329..331,367)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133151"
     Site            299..301
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133151"
     Site            299
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133151"
     Site            order(329..332,335..338)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133151"
     CDS             1..516
                     /gene="LOC144115663"
                     /coded_by="XM_077650117.1:1..1551"
                     /db_xref="GeneID:144115663"
ORIGIN      
        1 mtslqlptfd dtsdkwktyl iraeayfean aitdskqqra llvaalstqt vqvlagriap
       61 rtpnsltyde avaalnsyyd pkrheiaesy kfftrcqqeg eavraflvei rriadgcnfg
      121 saldrmlrdr ivcgvrstnl rkqllakkdl tleeaealai aaetaendan disgapptvl
      181 rmqashrsss rdtsgaaqec grcgstkhdd nacrwanarc frcrqrghla kkcyrartde
      241 naarrtasvk tltvetasrn enkdeahiwt lisgrkngle pplrrtfrwg eidldmevdt
      301 gspvcvisrn vydrhraqwp rlksphvkls cyagqlpvlg elelpvqfkn vsvtcplvvl
      361 dcsgpslcgr dliaalgktg vtmeeltapd sttgntpsdh madrpkrrkv tfrdasrlsa
      421 afptrhmspt qscglvvgrs rldhlarqqr iraklrhrqk mgawegtdhf ggengnseha
      481 nrssaaprrp sppssrvyar dcvcrghcks avrrrs
//
LOCUS       XP_077506244             460 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115663 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077506244
VERSION     XP_077506244.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650118.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..460
                     /product="uncharacterized protein LOC144115663 isoform X3"
                     /calculated_mol_wt=50774
     Region          182..>233
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     Region          294..374
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(299,301,303,329..331,367)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133151"
     Site            299..301
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133151"
     Site            299
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133151"
     Site            order(329..332,335..338)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133151"
     CDS             1..460
                     /gene="LOC144115663"
                     /coded_by="XM_077650118.1:1..1383"
                     /db_xref="GeneID:144115663"
ORIGIN      
        1 mtslqlptfd dtsdkwktyl iraeayfean aitdskqqra llvaalstqt vqvlagriap
       61 rtpnsltyde avaalnsyyd pkrheiaesy kfftrcqqeg eavraflvei rriadgcnfg
      121 saldrmlrdr ivcgvrstnl rkqllakkdl tleeaealai aaetaendan disgapptvl
      181 rmqashrsss rdtsgaaqec grcgstkhdd nacrwanarc frcrqrghla kkcyrartde
      241 naarrtasvk tltvetasrn enkdeahiwt lisgrkngle pplrrtfrwg eidldmevdt
      301 gspvcvisrn vydrhraqwp rlksphvkls cyagqlpvlg elelpvqfkn vsvtcplvvl
      361 dcsgpslcgr dliaalgktg vtmeeltapd sttgntpsdh mvnrllaeyk dvfsidqgli
      421 kgppaslklk etatpkfcgp pkekeghlpr cfsaiscvpd
//
LOCUS       XP_077506245             320 aa            linear   INV 25-NOV-2025
DEFINITION  visual pigment-like receptor peropsin isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506245
VERSION     XP_077506245.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650119.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..320
                     /product="visual pigment-like receptor peropsin isoform
                     X1"
                     /calculated_mol_wt=35131
     Region          18..297
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          19..43
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410628"
     Region          52..73
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410628"
     Region          89..111
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410628"
     Region          133..149
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410628"
     Region          177..200
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410628"
     Region          231..253
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410628"
     Region          265..290
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410628"
     CDS             1..320
                     /gene="LOC144115664"
                     /coded_by="XM_077650119.1:216..1178"
                     /db_xref="GeneID:144115664"
ORIGIN      
        1 mnnttealla tdgrrdvvvg syllavglvg svgngivlam yacvssvldp tslllislaa
       61 sdfgilvgcp fsaaasfsgr wlfgdggcqa yafmgfffgs ahigtltlla ldrylatcri
      121 gfrgkptyrr yvqllgivwf ygffwavmpl lgwaryglep sfqsctidwr hndasyksft
      181 vvyfvlgffv pasimvvcyr tsaahirvpk ptvvhrtdmn ddfwanqdsv ttmvvlivat
      241 ffaawtpyav lclwavfgnv asvphlvavl pplfcktasa inpfiyffsn priradiyal
      301 ltcrcrsmgr sscsieedyc
//
LOCUS       XP_077506246             281 aa            linear   INV 25-NOV-2025
DEFINITION  visual pigment-like receptor peropsin isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506246
VERSION     XP_077506246.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650120.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..281
                     /product="visual pigment-like receptor peropsin isoform
                     X2"
                     /calculated_mol_wt=31233
     Region          11..258
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          13..34
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410628"
     Region          50..72
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410628"
     Region          94..110
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410628"
     Region          138..161
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410628"
     Region          192..214
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410628"
     Region          226..251
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410628"
     CDS             1..281
                     /gene="LOC144115664"
                     /coded_by="XM_077650120.1:378..1223"
                     /db_xref="GeneID:144115664"
ORIGIN      
        1 myacvssvld ptslllisla asdfgilvgc pfsaaasfsg rwlfgdggcq ayafmgfffg
       61 sahigtltll aldrylatcr igfrgkptyr ryvqllgivw fygffwavmp llgwarygle
      121 psfqsctidw rhndasyksf tvvyfvlgff vpasimvvcy rtsaahirvp kptvvhrtdm
      181 nddfwanqds vttmvvliva tffaawtpya vlclwavfgn vasvphlvav lpplfcktas
      241 ainpfiyffs npriradiya lltcrcrsmg rsscsieedy c
//
LOCUS       XP_077506247             281 aa            linear   INV 25-NOV-2025
DEFINITION  visual pigment-like receptor peropsin isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506247
VERSION     XP_077506247.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650121.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..281
                     /product="visual pigment-like receptor peropsin isoform
                     X2"
                     /calculated_mol_wt=31233
     Region          11..258
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          13..34
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410628"
     Region          50..72
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410628"
     Region          94..110
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410628"
     Region          138..161
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410628"
     Region          192..214
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410628"
     Region          226..251
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410628"
     CDS             1..281
                     /gene="LOC144115664"
                     /coded_by="XM_077650121.1:131..976"
                     /db_xref="GeneID:144115664"
ORIGIN      
        1 myacvssvld ptslllisla asdfgilvgc pfsaaasfsg rwlfgdggcq ayafmgfffg
       61 sahigtltll aldrylatcr igfrgkptyr ryvqllgivw fygffwavmp llgwarygle
      121 psfqsctidw rhndasyksf tvvyfvlgff vpasimvvcy rtsaahirvp kptvvhrtdm
      181 nddfwanqds vttmvvliva tffaawtpya vlclwavfgn vasvphlvav lpplfcktas
      241 ainpfiyffs npriradiya lltcrcrsmg rsscsieedy c
//
LOCUS       XP_077506248             222 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115665 [Amblyomma americanum].
ACCESSION   XP_077506248
VERSION     XP_077506248.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650122.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..222
                     /product="uncharacterized protein LOC144115665"
                     /calculated_mol_wt=24742
     Region          7..91
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     Region          173..207
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..222
                     /gene="LOC144115665"
                     /coded_by="XM_077650122.1:104..772"
                     /db_xref="GeneID:144115665"
ORIGIN      
        1 masfneklvq evkkhkqlwd qqsklhkeag yreaawmeia aalvvtveec qtrwrtirda
       61 ylkrkkrrri dpkgqwsvld relgfldhfl rprtrrwaln gaaravspae vavereqpde
      121 ddemreaaaa eaaavavapa apaqpcpvam rpaavlphhl aaggaaaqec ssdpeelfcl
      181 slaadlkrll prerhmarmk mlqtlhdlef genigeaalg ep
//
LOCUS       XP_077506249             211 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115666 [Amblyomma americanum].
ACCESSION   XP_077506249
VERSION     XP_077506249.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650123.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..211
                     /product="uncharacterized protein LOC144115666"
                     /calculated_mol_wt=23603
     Region          7..91
                     /region_name="MADF"
                     /note="subfamily of SANT domain; smart00595"
                     /db_xref="CDD:214738"
     Region          162..196
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..211
                     /gene="LOC144115666"
                     /coded_by="XM_077650123.1:101..736"
                     /db_xref="GeneID:144115666"
ORIGIN      
        1 masfneklvq evkkhtqlwd qhsklhkeag yreaawmeia aalavtveec qtrwrtlrdt
       61 ylkrkkrrri dpkgqwsvld relgflddfl rprtrrstln aaapadvevk qeqpgedaem
      121 reagvaaaaa ppqscplamr paavmphrla aggaaaqals sdpeelfcls laarlkrllp
      181 rerhmarmkm lqtlhdlesg enigefalgd l
//
LOCUS       XP_077506250             192 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115667 [Amblyomma americanum].
ACCESSION   XP_077506250
VERSION     XP_077506250.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650124.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..192
                     /product="uncharacterized protein LOC144115667"
                     /calculated_mol_wt=20700
     CDS             1..192
                     /gene="LOC144115667"
                     /coded_by="XM_077650124.1:79..657"
                     /db_xref="GeneID:144115667"
ORIGIN      
        1 mlrstphyae rsssnvppee disiltspls snspssprlv ascisspaaa lfqemysdsp
       61 ndftdeddlr lpstsegaas aagssglsts qagaaapdpg sakrrasdvp ssrtkrrqsd
      121 sfddavkdvl rscsatllel kkknempapk ddsqeaahwi aaklrmlphk krcevtfqih
      181 kllyeaemeq ye
//
LOCUS       XP_077506251             174 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115669 [Amblyomma americanum].
ACCESSION   XP_077506251
VERSION     XP_077506251.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650125.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..174
                     /product="uncharacterized protein LOC144115669"
                     /calculated_mol_wt=20493
     CDS             1..174
                     /gene="LOC144115669"
                     /coded_by="XM_077650125.1:113..637"
                     /db_xref="GeneID:144115669"
ORIGIN      
        1 magsndevla tvnvvilacs lllhrrrarn arkrrfwvrp mfrrreqegq arlllprlra
       61 edeayfrdyl rmppstfdtl lgfvehslsr qvtpfrdpis ahdrlaitlr flangdtfrs
      121 lsynfligrs tasvlirett aaiwknlwht yvpfpqteie wrkihicrsl plww
//
LOCUS       XP_077506252             376 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115670 [Amblyomma americanum].
ACCESSION   XP_077506252
VERSION     XP_077506252.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650126.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..376
                     /product="uncharacterized protein LOC144115670"
                     /calculated_mol_wt=41798
     Region          187..264
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          205..209
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409549"
     Region          218..222
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409549"
     Region          243..247
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409549"
     Region          257..262
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409549"
     Region          314..349
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     CDS             1..376
                     /gene="LOC144115670"
                     /coded_by="XM_077650126.1:28..1158"
                     /db_xref="GeneID:144115670"
ORIGIN      
        1 mtrplplllp vvlvaaaasg arcpqdqdrw dasnkaltas lvfqgrllsk sedavgnlla
       61 rfrvqkvlkp mptaglrrrq evvvalvksr pppvhlnrsa sraprrdaln ealaprrrtl
      121 cpvsvpqgfv fnarylvyan pvagraarrl llpaaanfsa aatpdhytrr agravkkvvc
      181 ngcvlkpaqa kllrptsrvd ayrrlqlrck lngnpvpwvq wyfmgkpvtp narvhivtkw
      241 rrsrleiksa rrqdsglyec rawnvlrkep stvsvsvtvr drypppppqp eptratastt
      301 tndstlwplk grpcpltsyc lnggtcifye avkepvcqca egfigqrcen kdtaslktap
      361 pefdttiqmm qsywir
//
LOCUS       XP_077506253             691 aa            linear   INV 25-NOV-2025
DEFINITION  poly(ADP-ribose) glycohydrolase-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506253
VERSION     XP_077506253.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650127.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..691
                     /product="poly(ADP-ribose) glycohydrolase-like isoform X1"
                     /calculated_mol_wt=76998
     Region          309..639
                     /region_name="PARG_cat"
                     /note="Poly (ADP-ribose) glycohydrolase (PARG); pfam05028"
                     /db_xref="CDD:461528"
     CDS             1..691
                     /gene="LOC144115671"
                     /coded_by="XM_077650127.1:242..2317"
                     /db_xref="GeneID:144115671"
ORIGIN      
        1 msdglgpsrk rfkqvdlrsf lgemretcea cratgektek ntsrendqcs hvpckeessg
       61 qspafarkld sskledldss qelfpeldas nsqasghssd sentvfdlss sektvessql
      121 sqrdldpent hvaggaehga tscfdsqcfh vedrtipcic kgtsdsqess hseesipdes
      181 tipasfrrtp gcgvlcpdmg hshtvlcrvt rdeaghlkpy plvphprlpv drwdgvhvrm
      241 pcspqsqypt eqglngasvl rsrweliets llgdivdshs lvnairqyng rasvewnfic
      301 lhkflgqeld aresksffkv llpkmialal rlpvlitqpi pllksgqdht lsmsqlqagc
      361 llanaffctf prrntlksns eycnypdinf nrlfsgpyne arkmeklkci inyfrritqq
      421 epsgmltfhr rclaqpfewa rakselsnvf vsasgfiere ghgmlqvdfa nkfvgggvlg
      481 ggcvqeeirf mmcpelivsr lftevlgsre vlvitgaeqf nstsgyadkf lwkcdfkdqi
      541 prdswgrkct evvamdalcf atpgeqyrpv sirrelnkay cgfmcpelpr tqrsaiatgn
      601 wgcgafrgdp qlkaiiqvma asaagrdlvy ftfgdwqlcq slqsmlhflr srgitvggly
      661 kllveydsdq mlpagkpkgq lfeylystcs s
//
LOCUS       XP_077506255             689 aa            linear   INV 25-NOV-2025
DEFINITION  poly(ADP-ribose) glycohydrolase-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506255
VERSION     XP_077506255.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650129.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..689
                     /product="poly(ADP-ribose) glycohydrolase-like isoform X2"
                     /calculated_mol_wt=76769
     Region          307..637
                     /region_name="PARG_cat"
                     /note="Poly (ADP-ribose) glycohydrolase (PARG); pfam05028"
                     /db_xref="CDD:461528"
     CDS             1..689
                     /gene="LOC144115671"
                     /coded_by="XM_077650129.1:241..2310"
                     /db_xref="GeneID:144115671"
ORIGIN      
        1 msdglgpsrk rfkqvdlrsf lgemretcea cratgektek ntsrendqcs hvpckeessg
       61 qspafarkld sskledldss qelfpeldas nsqasghssd sentvfdlss sektvessql
      121 sqrdldpent hvaggaehga tscfdsqcfh drtipcickg tsdsqesshs eesipdesti
      181 pasfrrtpgc gvlcpdmghs htvlcrvtrd eaghlkpypl vphprlpvdr wdgvhvrmpc
      241 spqsqypteq glngasvlrs rwelietsll gdivdshslv nairqyngra svewnficlh
      301 kflgqeldar esksffkvll pkmialalrl pvlitqpipl lksgqdhtls msqlqagcll
      361 anaffctfpr rntlksnsey cnypdinfnr lfsgpynear kmeklkciin yfrritqqep
      421 sgmltfhrrc laqpfewara kselsnvfvs asgfieregh gmlqvdfank fvgggvlggg
      481 cvqeeirfmm cpelivsrlf tevlgsrevl vitgaeqfns tsgyadkflw kcdfkdqipr
      541 dswgrkctev vamdalcfat pgeqyrpvsi rrelnkaycg fmcpelprtq rsaiatgnwg
      601 cgafrgdpql kaiiqvmaas aagrdlvyft fgdwqlcqsl qsmlhflrsr gitvgglykl
      661 lveydsdqml pagkpkgqlf eylystcss
//
LOCUS       XP_077506256             686 aa            linear   INV 25-NOV-2025
DEFINITION  poly(ADP-ribose) glycohydrolase-like isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077506256
VERSION     XP_077506256.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650130.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..686
                     /product="poly(ADP-ribose) glycohydrolase-like isoform X3"
                     /calculated_mol_wt=76436
     Region          304..634
                     /region_name="PARG_cat"
                     /note="Poly (ADP-ribose) glycohydrolase (PARG); pfam05028"
                     /db_xref="CDD:461528"
     CDS             1..686
                     /gene="LOC144115671"
                     /coded_by="XM_077650130.1:245..2305"
                     /db_xref="GeneID:144115671"
ORIGIN      
        1 msdglgpsrk rfkqvdlrsf lgemretcea cratgektek ntsrendqcs hvpckeessg
       61 qspafarkld sskledldss qelfpeldas nsqasghssd sentssektv essqlsqrdl
      121 dpenthvagg aehgatscfd sqcfhvedrt ipcickgtsd sqesshsees ipdestipas
      181 frrtpgcgvl cpdmghshtv lcrvtrdeag hlkpyplvph prlpvdrwdg vhvrmpcspq
      241 sqypteqgln gasvlrsrwe lietsllgdi vdshslvnai rqyngrasve wnficlhkfl
      301 gqeldaresk sffkvllpkm ialalrlpvl itqpipllks gqdhtlsmsq lqagcllana
      361 ffctfprrnt lksnseycny pdinfnrlfs gpynearkme klkciinyfr ritqqepsgm
      421 ltfhrrclaq pfewarakse lsnvfvsasg fiereghgml qvdfankfvg ggvlgggcvq
      481 eeirfmmcpe livsrlftev lgsrevlvit gaeqfnstsg yadkflwkcd fkdqiprdsw
      541 grkctevvam dalcfatpge qyrpvsirre lnkaycgfmc pelprtqrsa iatgnwgcga
      601 frgdpqlkai iqvmaasaag rdlvyftfgd wqlcqslqsm lhflrsrgit vgglykllve
      661 ydsdqmlpag kpkgqlfeyl ystcss
//
LOCUS       XP_077506257             139 aa            linear   INV 25-NOV-2025
DEFINITION  ribosomal protein S24 isoform X1 [Amblyomma americanum].
ACCESSION   XP_077506257
VERSION     XP_077506257.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650131.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..139
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..139
                     /product="ribosomal protein S24 isoform X1"
                     /calculated_mol_wt=15760
     Region          4..138
                     /region_name="PTZ00071"
                     /note="40S ribosomal protein S24; Provisional"
                     /db_xref="CDD:240256"
     CDS             1..139
                     /gene="RpS24"
                     /coded_by="XM_077650131.1:96..515"
                     /db_xref="GeneID:144115673"
ORIGIN      
        1 madssvtvrt rkymtnrllc rkqmqedsvv vdiihpnrgt vsktevrekl samynttpdv
       61 vfcfgfrtqf gggktsgfal iydtldyakk fepkyrlcrn glgekgrtgr kqrkerknrm
      121 kkvrgvkkak vgassgkkr
//
LOCUS       XP_077506258             138 aa            linear   INV 25-NOV-2025
DEFINITION  ribosomal protein S24 isoform X2 [Amblyomma americanum].
ACCESSION   XP_077506258
VERSION     XP_077506258.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650132.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..138
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..138
                     /product="ribosomal protein S24 isoform X2"
                     /calculated_mol_wt=15604
     Region          4..138
                     /region_name="PTZ00071"
                     /note="40S ribosomal protein S24; Provisional"
                     /db_xref="CDD:240256"
     CDS             1..138
                     /gene="RpS24"
                     /coded_by="XM_077650132.1:96..512"
                     /db_xref="GeneID:144115673"
ORIGIN      
        1 madssvtvrt rkymtnrllc rkqmqedsvv vdiihpnrgt vsktevrekl samynttpdv
       61 vfcfgfrtqf gggktsgfal iydtldyakk fepkyrlcrn glgekgrtgr kqrkerknrm
      121 kkvrgvkkak vgassgkk
//
LOCUS       XP_077506259             134 aa            linear   INV 25-NOV-2025
DEFINITION  ribosomal protein S24 isoform X3 [Amblyomma americanum].
ACCESSION   XP_077506259
VERSION     XP_077506259.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650133.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..134
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..134
                     /product="ribosomal protein S24 isoform X3"
                     /calculated_mol_wt=15202
     Region          4..133
                     /region_name="PTZ00071"
                     /note="40S ribosomal protein S24; Provisional"
                     /db_xref="CDD:240256"
     CDS             1..134
                     /gene="RpS24"
                     /coded_by="XM_077650133.1:96..500"
                     /db_xref="GeneID:144115673"
ORIGIN      
        1 madssvtvrt rkymtnrllc rkqmvvdiih pnrgtvskte vreklsamyn ttpdvvfcfg
       61 frtqfgggkt sgfaliydtl dyakkfepky rlcrnglgek grtgrkqrke rknrmkkvrg
      121 vkkakvgass gkkr
//
LOCUS       XP_077506260             432 aa            linear   INV 25-NOV-2025
DEFINITION  AP-3 complex subunit mu-2-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077506260
VERSION     XP_077506260.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650134.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..432
                     /product="AP-3 complex subunit mu-2-like isoform X1"
                     /calculated_mol_wt=48580
     Region          3..141
                     /region_name="AP3_Mu_N"
                     /note="AP-3 complex subunit mu N-terminal domain; cd14837"
                     /db_xref="CDD:341441"
     Site            order(18,21,46..47,55,57,59,71..73,75..77,80,84,110..111,
                     114..115,117..119,121..124,133,135..136)
                     /site_type="other"
                     /note="putative AP-3 beta interface [polypeptide binding]"
                     /db_xref="CDD:341441"
     Region          165..432
                     /region_name="AP_MHD_Cterm"
                     /note="C-terminal domain of adaptor protein (AP) complexes
                     medium mu subunits and its homologs (MHD); cl10970"
                     /db_xref="CDD:472082"
     CDS             1..432
                     /gene="LOC144115674"
                     /coded_by="XM_077650134.1:338..1636"
                     /db_xref="GeneID:144115674"
ORIGIN      
        1 mihsvfving sgdvfmekhw ksvihrsvcd yffdvqkkaa spedippvis aphhyliniy
       61 rnkmffvavt msevpplfvi eflhrvvdtf vdyfsdctef likehyvvvy elldemldng
      121 fplatesnil kelikppnil rtlantvtgr tnlsstlptg qlsnvpwrrt gvkyanneay
      181 fdvveevdai idksgsvisa eiqgyvdcci klsgmpdlsl tfvnprlfdd vsfhpcvrfr
      241 rweservlsf vppdgnfrlm syhigsqrnr myctlnglhe fptvvavpvy vrhqisfrea
      301 gggrldisig pkqtmgktvd evvlevplck tvlnvtltas qgkhsfdpvs knlvwevgri
      361 epgrlpnlrg smalqagapp pdanptitvr ftinplavsg lkvnrldmyg ekykpfkgvk
      421 yvtkagrfqv rt
//
LOCUS       XP_077506261             417 aa            linear   INV 25-NOV-2025
DEFINITION  AP-3 complex subunit mu-2-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077506261
VERSION     XP_077506261.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650135.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..417
                     /product="AP-3 complex subunit mu-2-like isoform X2"
                     /calculated_mol_wt=46733
     Region          3..141
                     /region_name="AP3_Mu_N"
                     /note="AP-3 complex subunit mu N-terminal domain; cd14837"
                     /db_xref="CDD:341441"
     Site            order(18,21,46..47,55,57,59,71..73,75..77,80,84,110..111,
                     114..115,117..119,121..124,133,135..136)
                     /site_type="other"
                     /note="putative AP-3 beta interface [polypeptide binding]"
                     /db_xref="CDD:341441"
     Region          165..417
                     /region_name="AP_MHD_Cterm"
                     /note="C-terminal domain of adaptor protein (AP) complexes
                     medium mu subunits and its homologs (MHD); cl10970"
                     /db_xref="CDD:472082"
     CDS             1..417
                     /gene="LOC144115674"
                     /coded_by="XM_077650135.1:338..1591"
                     /db_xref="GeneID:144115674"
ORIGIN      
        1 mihsvfving sgdvfmekhw ksvihrsvcd yffdvqkkaa spedippvis aphhyliniy
       61 rnkmffvavt msevpplfvi eflhrvvdtf vdyfsdctef likehyvvvy elldemldng
      121 fplatesnil kelikppnil rtlantvtgr tnlsstlptg qlsnvpwrrt gvkyanneay
      181 fdvveevdai idksgsvisa eiqgyvdcci klsgmpdlsl tfvnprlfdd vsfhpcvrfr
      241 rweservlsf vppdgnfrlm syhigsqsvv avpvyvrhqi sfreagggrl disigpkqtm
      301 gktvdevvle vplcktvlnv tltasqgkhs fdpvsknlvw evgriepgrl pnlrgsmalq
      361 agapppdanp titvrftinp lavsglkvnr ldmygekykp fkgvkyvtka grfqvrt
//
LOCUS       XP_077506262             952 aa            linear   INV 25-NOV-2025
DEFINITION  RING finger and CCCH-type zinc finger domain-containing protein
            roquin isoform X1 [Amblyomma americanum].
ACCESSION   XP_077506262
VERSION     XP_077506262.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650136.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..952
                     /product="RING finger and CCCH-type zinc finger
                     domain-containing protein roquin isoform X1"
                     /calculated_mol_wt=103789
     Region          243..298
                     /region_name="ROQ_II"
                     /note="Roquin II domain; pfam18386"
                     /db_xref="CDD:436456"
     Region          402..428
                     /region_name="zf-CCCH"
                     /note="Zinc finger C-x8-C-x5-C-x3-H type (and similar);
                     pfam00642"
                     /db_xref="CDD:459885"
     CDS             1..952
                     /gene="roq"
                     /coded_by="XM_077650136.1:657..3515"
                     /db_xref="GeneID:144115676"
ORIGIN      
        1 mpvqapppps wgappaaccc grgphtplch lqqqrrpdns vptllhlvaa psaapappva
       61 vvpeesaeqa varcihemal llrpatqggc wlsrplqrkl vslvhcplse aegraraara
      121 arslgercal elllqhhqhq agggagggqq lsaalwaavr argcqflgph mqeevlrlvl
      181 laledgspls rkvlvlfvvq rlaphfpqas ktsighvvql lyrascfkvs kregdsslmq
      241 lkeefctyea lrrehdaqiv qiaseaglri apeqwsslly gdaahrshmq slvdklqspq
      301 sfsqsvhels lalqrtgdpa glaalrphfd llahwepdga aptweelsrc vlaaraclqg
      361 lvaflgafgg rranggtpss pgrggradgg spchasgggp ryktslcrdv iqrgscprga
      421 hctfahsqee mdsrvfysrq edrfrkkspp hrddrfgksa hqedfrkkss hhqedrvier
      481 ppprqggrfv mnssphqkgr vverspprqr srfvmkspph qegrviersp prqggrfvmk
      541 ssphqegrvi erspprqrsr fvmkspphqe drviersppr qggrfvmkss pchegraken
      601 spphregrvi erspprqegr fimksspyqe grvlersppq qegrfvmksp lhqgrgvvvy
      661 rarrpprtst dpkpvvnsda sisanrcrea sgspehqcas gppivgvsrr nspppalapp
      721 tlrlgaeplp psvvglnpes pafqpeepwr npsplcredd ddwgsshgsc dsstsshhpt
      781 rpcpseedpl vpfdpplvsk ygpisrcars lvrgpapiql lqeaccqfrd cleqkrkvts
      841 rqpqeltspt aalrlplasa aaapsfyaqg ftllptgdla cstdprlfpl kgapsfapap
      901 gallaappal vapptkgses wagqpqvpts mgpwllnqle esslcadscw ph
//
LOCUS       XP_077506263             939 aa            linear   INV 25-NOV-2025
DEFINITION  RING finger and CCCH-type zinc finger domain-containing protein
            roquin isoform X2 [Amblyomma americanum].
ACCESSION   XP_077506263
VERSION     XP_077506263.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650137.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..939
                     /product="RING finger and CCCH-type zinc finger
                     domain-containing protein roquin isoform X2"
                     /calculated_mol_wt=102089
     Region          243..298
                     /region_name="ROQ_II"
                     /note="Roquin II domain; pfam18386"
                     /db_xref="CDD:436456"
     Region          402..428
                     /region_name="zf-CCCH"
                     /note="Zinc finger C-x8-C-x5-C-x3-H type (and similar);
                     pfam00642"
                     /db_xref="CDD:459885"
     CDS             1..939
                     /gene="roq"
                     /coded_by="XM_077650137.1:657..3476"
                     /db_xref="GeneID:144115676"
ORIGIN      
        1 mpvqapppps wgappaaccc grgphtplch lqqqrrpdns vptllhlvaa psaapappva
       61 vvpeesaeqa varcihemal llrpatqggc wlsrplqrkl vslvhcplse aegraraara
      121 arslgercal elllqhhqhq agggagggqq lsaalwaavr argcqflgph mqeevlrlvl
      181 laledgspls rkvlvlfvvq rlaphfpqas ktsighvvql lyrascfkvs kregdsslmq
      241 lkeefctyea lrrehdaqiv qiaseaglri apeqwsslly gdaahrshmq slvdklqspq
      301 sfsqsvhels lalqrtgdpa glaalrphfd llahwepdga aptweelsrc vlaaraclqg
      361 lvaflgafgg rranggtpss pgrggradgg spchasgggp ryktslcrdv iqrgscprga
      421 hctfahsqee mdrkspphrd drfgksahqe dfrkksshhq edrvierppp rqggrfvmns
      481 sphqkgrvve rspprqrsrf vmkspphqeg rvierspprq ggrfvmkssp hqegrviers
      541 pprqrsrfvm kspphqedrv ierspprqgg rfvmksspch egrakenspp hregrviers
      601 pprqegrfim ksspyqegrv lersppqqeg rfvmksplhq grgvvvyrar rpprtstdpk
      661 pvvnsdasis anrcreasgs pehqcasgpp ivgvsrrnsp ppalapptlr lgaeplppsv
      721 vglnpespaf qpeepwrnps plcreddddw gsshgscdss tsshhptrpc pseedplvpf
      781 dpplvskygp isrcarslvr gpapiqllqe accqfrdcle qkrkvtsrqp qeltsptaal
      841 rlplasaaaa psfyaqgftl lptgdlacst dprlfplkga psfapapgal laappalvap
      901 ptkgseswag qpqvptsmgp wllnqleess lcadscwph
//
LOCUS       XP_077506264             934 aa            linear   INV 25-NOV-2025
DEFINITION  RING finger and CCCH-type zinc finger domain-containing protein
            roquin isoform X3 [Amblyomma americanum].
ACCESSION   XP_077506264
VERSION     XP_077506264.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650138.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..934
                     /product="RING finger and CCCH-type zinc finger
                     domain-containing protein roquin isoform X3"
                     /calculated_mol_wt=101596
     Region          243..298
                     /region_name="ROQ_II"
                     /note="Roquin II domain; pfam18386"
                     /db_xref="CDD:436456"
     Region          402..428
                     /region_name="zf-CCCH"
                     /note="Zinc finger C-x8-C-x5-C-x3-H type (and similar);
                     pfam00642"
                     /db_xref="CDD:459885"
     CDS             1..934
                     /gene="roq"
                     /coded_by="XM_077650138.1:657..3461"
                     /db_xref="GeneID:144115676"
ORIGIN      
        1 mpvqapppps wgappaaccc grgphtplch lqqqrrpdns vptllhlvaa psaapappva
       61 vvpeesaeqa varcihemal llrpatqggc wlsrplqrkl vslvhcplse aegraraara
      121 arslgercal elllqhhqhq agggagggqq lsaalwaavr argcqflgph mqeevlrlvl
      181 laledgspls rkvlvlfvvq rlaphfpqas ktsighvvql lyrascfkvs kregdsslmq
      241 lkeefctyea lrrehdaqiv qiaseaglri apeqwsslly gdaahrshmq slvdklqspq
      301 sfsqsvhels lalqrtgdpa glaalrphfd llahwepdga aptweelsrc vlaaraclqg
      361 lvaflgafgg rranggtpss pgrggradgg spchasgggp ryktslcrdv iqrgscprga
      421 hctfahsqee mdsrvfysrq edrfrkkspp hrddrfgksa hqedfrkkss hhqedrvier
      481 ppprqggrfv mnssphqkgr vverspprqr srfvmkspph qegrviersp prqggrfvmk
      541 ssphqegrvi erspprqrsr fvmkspphqe drviersppr qggrfvmkss pchegraken
      601 spphregrvi erspprqegr fimksspyqe grvlersppq qegrfvmksp lhqgrgvvvy
      661 rarrpprtst dpkpvvnsda sisanrcrea sgspehqcas gppivgvsrr nspppalapp
      721 tlrlgaeplp psvvglnpes pafqpeepwr npsplcredd ddwgsshgsc dsstsshhpt
      781 rpcpseedpl vpfdpplvsk ygpisrcars lvrgpapiqv tsrqpqelts ptaalrlpla
      841 saaaapsfya qgftllptgd lacstdprlf plkgapsfap apgallaapp alvapptkgs
      901 eswagqpqvp tsmgpwllnq leesslcads cwph
//
LOCUS       XP_077506266             916 aa            linear   INV 25-NOV-2025
DEFINITION  RING finger and CCCH-type zinc finger domain-containing protein
            roquin isoform X4 [Amblyomma americanum].
ACCESSION   XP_077506266
VERSION     XP_077506266.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650140.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..916
                     /product="RING finger and CCCH-type zinc finger
                     domain-containing protein roquin isoform X4"
                     /calculated_mol_wt=99644
     Region          243..298
                     /region_name="ROQ_II"
                     /note="Roquin II domain; pfam18386"
                     /db_xref="CDD:436456"
     Region          402..428
                     /region_name="zf-CCCH"
                     /note="Zinc finger C-x8-C-x5-C-x3-H type (and similar);
                     pfam00642"
                     /db_xref="CDD:459885"
     CDS             1..916
                     /gene="roq"
                     /coded_by="XM_077650140.1:657..3407"
                     /db_xref="GeneID:144115676"
ORIGIN      
        1 mpvqapppps wgappaaccc grgphtplch lqqqrrpdns vptllhlvaa psaapappva
       61 vvpeesaeqa varcihemal llrpatqggc wlsrplqrkl vslvhcplse aegraraara
      121 arslgercal elllqhhqhq agggagggqq lsaalwaavr argcqflgph mqeevlrlvl
      181 laledgspls rkvlvlfvvq rlaphfpqas ktsighvvql lyrascfkvs kregdsslmq
      241 lkeefctyea lrrehdaqiv qiaseaglri apeqwsslly gdaahrshmq slvdklqspq
      301 sfsqsvhels lalqrtgdpa glaalrphfd llahwepdga aptweelsrc vlaaraclqg
      361 lvaflgafgg rranggtpss pgrggradgg spchasgggp ryktslcrdv iqrgscprga
      421 hctfahsqee mdsrvfysrq edrfrkkspp hrddrfgksa hqedfrkrsp prqrsrfvmk
      481 spphqegrvi erspprqggr fvmkssphqe grviersppr qrsrfvmksp phqedrvier
      541 spprqggrfv mksspchegr akenspphre grviersppr qegrfimkss pyqegrvler
      601 sppqqegrfv mksplhqgrg vvvyrarrpp rtstdpkpvv nsdasisanr creasgspeh
      661 qcasgppivg vsrrnspppa lapptlrlga eplppsvvgl npespafqpe epwrnpsplc
      721 reddddwgss hgscdsstss hhptrpcpse edplvpfdpp lvskygpisr carslvrgpa
      781 piqllqeacc qfrdcleqkr kvtsrqpqel tsptaalrlp lasaaaapsf yaqgftllpt
      841 gdlacstdpr lfplkgapsf apapgallaa ppalvapptk gseswagqpq vptsmgpwll
      901 nqleesslca dscwph
//
LOCUS       XP_077506267             903 aa            linear   INV 25-NOV-2025
DEFINITION  RING finger and CCCH-type zinc finger domain-containing protein
            roquin isoform X5 [Amblyomma americanum].
ACCESSION   XP_077506267
VERSION     XP_077506267.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650141.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..903
                     /product="RING finger and CCCH-type zinc finger
                     domain-containing protein roquin isoform X5"
                     /calculated_mol_wt=97944
     Region          243..298
                     /region_name="ROQ_II"
                     /note="Roquin II domain; pfam18386"
                     /db_xref="CDD:436456"
     Region          402..428
                     /region_name="zf-CCCH"
                     /note="Zinc finger C-x8-C-x5-C-x3-H type (and similar);
                     pfam00642"
                     /db_xref="CDD:459885"
     CDS             1..903
                     /gene="roq"
                     /coded_by="XM_077650141.1:657..3368"
                     /db_xref="GeneID:144115676"
ORIGIN      
        1 mpvqapppps wgappaaccc grgphtplch lqqqrrpdns vptllhlvaa psaapappva
       61 vvpeesaeqa varcihemal llrpatqggc wlsrplqrkl vslvhcplse aegraraara
      121 arslgercal elllqhhqhq agggagggqq lsaalwaavr argcqflgph mqeevlrlvl
      181 laledgspls rkvlvlfvvq rlaphfpqas ktsighvvql lyrascfkvs kregdsslmq
      241 lkeefctyea lrrehdaqiv qiaseaglri apeqwsslly gdaahrshmq slvdklqspq
      301 sfsqsvhels lalqrtgdpa glaalrphfd llahwepdga aptweelsrc vlaaraclqg
      361 lvaflgafgg rranggtpss pgrggradgg spchasgggp ryktslcrdv iqrgscprga
      421 hctfahsqee mdrkspphrd drfgksahqe dfrkrspprq rsrfvmkspp hqegrviers
      481 pprqggrfvm kssphqegrv ierspprqrs rfvmkspphq edrvierspp rqggrfvmks
      541 spchegrake nspphregrv ierspprqeg rfimksspyq egrvlerspp qqegrfvmks
      601 plhqgrgvvv yrarrpprts tdpkpvvnsd asisanrcre asgspehqca sgppivgvsr
      661 rnspppalap ptlrlgaepl ppsvvglnpe spafqpeepw rnpsplcred dddwgsshgs
      721 cdsstsshhp trpcpseedp lvpfdpplvs kygpisrcar slvrgpapiq llqeaccqfr
      781 dcleqkrkvt srqpqeltsp taalrlplas aaaapsfyaq gftllptgdl acstdprlfp
      841 lkgapsfapa pgallaappa lvapptkgse swagqpqvpt smgpwllnql eesslcadsc
      901 wph
//
LOCUS       XP_077506268             885 aa            linear   INV 25-NOV-2025
DEFINITION  RING finger and CCCH-type zinc finger domain-containing protein
            roquin isoform X6 [Amblyomma americanum].
ACCESSION   XP_077506268
VERSION     XP_077506268.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650142.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..885
                     /product="RING finger and CCCH-type zinc finger
                     domain-containing protein roquin isoform X6"
                     /calculated_mol_wt=97389
     Region          243..298
                     /region_name="ROQ_II"
                     /note="Roquin II domain; pfam18386"
                     /db_xref="CDD:436456"
     Region          402..428
                     /region_name="zf-CCCH"
                     /note="Zinc finger C-x8-C-x5-C-x3-H type (and similar);
                     pfam00642"
                     /db_xref="CDD:459885"
     CDS             1..885
                     /gene="roq"
                     /coded_by="XM_077650142.1:657..3314"
                     /db_xref="GeneID:144115676"
ORIGIN      
        1 mpvqapppps wgappaaccc grgphtplch lqqqrrpdns vptllhlvaa psaapappva
       61 vvpeesaeqa varcihemal llrpatqggc wlsrplqrkl vslvhcplse aegraraara
      121 arslgercal elllqhhqhq agggagggqq lsaalwaavr argcqflgph mqeevlrlvl
      181 laledgspls rkvlvlfvvq rlaphfpqas ktsighvvql lyrascfkvs kregdsslmq
      241 lkeefctyea lrrehdaqiv qiaseaglri apeqwsslly gdaahrshmq slvdklqspq
      301 sfsqsvhels lalqrtgdpa glaalrphfd llahwepdga aptweelsrc vlaaraclqg
      361 lvaflgafgg rranggtpss pgrggradgg spchasgggp ryktslcrdv iqrgscprga
      421 hctfahsqee mdsrvfysrq edrfrkkspp hrddrfgksa hqedfrkkss hhqedrvier
      481 ppprqggrfv mnssphqkgr vverspprqr srfvmkspph qegrviersp prqggrfvmk
      541 ssphqegrvi erspprqrsr fvmkspphqe drviersppr qggrfvmkss pchegraken
      601 spphregrvi erspprqegr fimksspyqe grvlersppq qegrfvmksp lhqgrgvvvy
      661 rarrpprtst dpkpvvnsda sisanrcrea sgspehqcas gppivgvsrr nspppalapp
      721 tlrlgaeplp psvvglnpes pafqpeepwr npsplcredd ddwgsshgsc dsstsshhpt
      781 rpcpseedpl vpfdpplvsk ygpisrcars lvrgpapiqv lpccrrviwh aaptpgsfps
      841 rghhplplhr epywlllqhw lhlqprgasp gqdslrcrhp wapgf
//
LOCUS       XP_077506269             316 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115677 [Amblyomma americanum].
ACCESSION   XP_077506269
VERSION     XP_077506269.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650143.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..316
                     /product="uncharacterized protein LOC144115677"
                     /calculated_mol_wt=34466
     Region          <116..>252
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          139..157
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          166..186
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          187..211
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          212..235
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          236..260
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          261..283
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          270..315
                     /region_name="LRRCT"
                     /note="Leucine rich repeat C-terminal domain; smart00082"
                     /db_xref="CDD:214507"
     CDS             1..316
                     /gene="LOC144115677"
                     /coded_by="XM_077650143.1:93..1043"
                     /db_xref="GeneID:144115677"
ORIGIN      
        1 mrhpvvvvvl lavalaaraa deagckdtsg tyetcytcsg fveprdferh ldrgrvprss
       61 rglsfilrds rpvdfpdgwl qgvnatllef qnvtfsspet alarlaggge ppatverlvf
      121 sydstlpasw ealrqlprlk tlglvkmsgl rlgpefgglp rsvrgvsvlh ssvasveggw
      181 laelpdleyv vvrdsrlaaf srdmlprpal klrsidldyn dlsaipakfs egfpalafld
      241 legnkittia eeelgavlnn tgtvvllghn rlhcdctlah lrrhsdrrlr archspdelr
      301 grsvqsltda elgcpr
//
LOCUS       XP_077506270             183 aa            linear   INV 25-NOV-2025
DEFINITION  NEDD8-conjugating enzyme Ubc12-like [Amblyomma americanum].
ACCESSION   XP_077506270
VERSION     XP_077506270.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650144.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="NEDD8-conjugating enzyme Ubc12-like"
                     /calculated_mol_wt=20916
     Region          29..166
                     /region_name="UBCc_UBE2F_UBE2M"
                     /note="Ubiquitin-conjugating enzyme E2, catalytic (UBCc)
                     domain of ubiquitin conjugating enzymes E2 F, E2 M and
                     related proteins; cd23794"
                     /db_xref="CDD:467414"
     CDS             1..183
                     /gene="LOC144115678"
                     /coded_by="XM_077650144.1:118..669"
                     /db_xref="GeneID:144115678"
ORIGIN      
        1 miklfslkqq qkdgeamgnk ptsqkrasaa qlritkdine lnlpktcqme fpdpddllnf
       61 klvicpdegf yrngrfvfsf rvspnyphep pkvkcdtmvy hpnidldgnv clnilredwk
      121 pvltvnsivy glqylflepn pedplnkdaa evlqsnrrlf eqnvkkamlg gyvgatyfer
      181 clk
//
LOCUS       XP_077506271             136 aa            linear   INV 25-NOV-2025
DEFINITION  DNA repair protein SWI5 homolog isoform X1 [Amblyomma americanum].
ACCESSION   XP_077506271
VERSION     XP_077506271.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650145.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..136
                     /product="DNA repair protein SWI5 homolog isoform X1"
                     /calculated_mol_wt=15175
     Region          53..125
                     /region_name="Swi5"
                     /note="pfam07061"
                     /db_xref="CDD:462077"
     CDS             1..136
                     /gene="LOC144115679"
                     /coded_by="XM_077650145.1:332..742"
                     /db_xref="GeneID:144115679"
ORIGIN      
        1 mseapeqats etptrnkfki qarrsrssla aanrpysspm kattvssesv edlqrkladl
       61 rqksrlldee ieqlqaegcs iedlpwhmdr lhiynqikdi ahqvigslav legvtirakq
      121 eyyglptter ggalhd
//
LOCUS       XP_077506272             136 aa            linear   INV 25-NOV-2025
DEFINITION  DNA repair protein SWI5 homolog isoform X1 [Amblyomma americanum].
ACCESSION   XP_077506272
VERSION     XP_077506272.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650146.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..136
                     /product="DNA repair protein SWI5 homolog isoform X1"
                     /calculated_mol_wt=15175
     Region          53..125
                     /region_name="Swi5"
                     /note="pfam07061"
                     /db_xref="CDD:462077"
     CDS             1..136
                     /gene="LOC144115679"
                     /coded_by="XM_077650146.1:249..659"
                     /db_xref="GeneID:144115679"
ORIGIN      
        1 mseapeqats etptrnkfki qarrsrssla aanrpysspm kattvssesv edlqrkladl
       61 rqksrlldee ieqlqaegcs iedlpwhmdr lhiynqikdi ahqvigslav legvtirakq
      121 eyyglptter ggalhd
//
LOCUS       XP_077506273             136 aa            linear   INV 25-NOV-2025
DEFINITION  DNA repair protein SWI5 homolog isoform X1 [Amblyomma americanum].
ACCESSION   XP_077506273
VERSION     XP_077506273.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650147.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..136
                     /product="DNA repair protein SWI5 homolog isoform X1"
                     /calculated_mol_wt=15175
     Region          53..125
                     /region_name="Swi5"
                     /note="pfam07061"
                     /db_xref="CDD:462077"
     CDS             1..136
                     /gene="LOC144115679"
                     /coded_by="XM_077650147.1:32..442"
                     /db_xref="GeneID:144115679"
ORIGIN      
        1 mseapeqats etptrnkfki qarrsrssla aanrpysspm kattvssesv edlqrkladl
       61 rqksrlldee ieqlqaegcs iedlpwhmdr lhiynqikdi ahqvigslav legvtirakq
      121 eyyglptter ggalhd
//
LOCUS       XP_077506274             114 aa            linear   INV 25-NOV-2025
DEFINITION  DNA repair protein SWI5 homolog isoform X2 [Amblyomma americanum].
ACCESSION   XP_077506274
VERSION     XP_077506274.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650148.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..114
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..114
                     /product="DNA repair protein SWI5 homolog isoform X2"
                     /calculated_mol_wt=12784
     Region          53..>106
                     /region_name="Swi5"
                     /note="pfam07061"
                     /db_xref="CDD:462077"
     CDS             1..114
                     /gene="LOC144115679"
                     /coded_by="XM_077650148.1:332..676"
                     /db_xref="GeneID:144115679"
ORIGIN      
        1 mseapeqats etptrnkfki qarrsrssla aanrpysspm kattvssesv edlqrkladl
       61 rqksrlldee ieqlqaegcs iedlpwhmdr lhiynqikdi ahqvigslgf fvvs
//
LOCUS       XP_077506275             358 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115681 [Amblyomma americanum].
ACCESSION   XP_077506275
VERSION     XP_077506275.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650149.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..358
                     /product="uncharacterized protein LOC144115681"
                     /calculated_mol_wt=39876
     Region          96..141
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          176..343
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..358
                     /gene="LOC144115681"
                     /coded_by="XM_077650149.1:214..1290"
                     /db_xref="GeneID:144115681"
ORIGIN      
        1 metetdlsmq nishlieeng rlkqrlasle eecsaakqka gelenkhtnh aiitkevvmq
       61 saktvkfytg fvstamftaf lqfvlsawkp scatwldaet qlimvlmrlr lglltqdlsc
      121 rfgisnatvs sffhawldvl aanmtkliiw psrqtlqanr paafqdpffd gvtgiidcte
      181 vfiqrpilmt arsqtyshyk hhntvkilvv ispsgaitfv skawggrtpd keitlrsgll
      241 dkvkegdvfl vdrgfrcgem faargatllm psltknraql pgaevttsrk vssvrihver
      301 aiqrlkvfri fqtvlplnfv krtgdknlas idkvvvvcsa lvnlqtpiik nsqrrsas
//
LOCUS       XP_077506276             236 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115682 [Amblyomma americanum].
ACCESSION   XP_077506276
VERSION     XP_077506276.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650150.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..236
                     /product="uncharacterized protein LOC144115682"
                     /calculated_mol_wt=25836
     Region          106..>212
                     /region_name="SWIM"
                     /note="Uncharacterized protein, contains SWIM-type Zn
                     finger domain [Function unknown]; COG4715"
                     /db_xref="CDD:443750"
     CDS             1..236
                     /gene="LOC144115682"
                     /coded_by="XM_077650150.1:364..1074"
                     /db_xref="GeneID:144115682"
ORIGIN      
        1 mssdyfkkld teaqvryrak lsfrgeelpd aldddvvrfa ftsgprnlpp vtvadiylyl
       61 vegvcfytne qfksykleda ynaficakvk qvksfkagah gdgvvviaat veasqtlsks
      121 ykawcivked gtiesahctc maglgeccth vaallfrvea taqyglndps ptdvackwie
      181 askgstaapv seieffkpki grappqvsea pdysvpvltd eqvsrflhqv kvrtsh
//
LOCUS       XP_077506278             484 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115683 [Amblyomma americanum].
ACCESSION   XP_077506278
VERSION     XP_077506278.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650152.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 21% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..484
                     /product="uncharacterized protein LOC144115683"
                     /calculated_mol_wt=53494
     CDS             1..484
                     /gene="LOC144115683"
                     /coded_by="XM_077650152.1:1..1455"
                     /db_xref="GeneID:144115683"
ORIGIN      
        1 mrrarewvlq ikfesvecsl vaynvpkehw aqlvfpvlae rvaylstrft aeqhrdydel
       61 kavvldvlkl saaeyqrrfs gatkrreetw kafvtrvqsy lnfylksrcv ssfdgllkll
      121 vadqlkvgls eeahkyvklr egekwlnade iaallqtyee aagkgsaqrk adsfkgfdtp
      181 ptprteatpt rkhgaergdr ivavrpggek kkvsrsggcf rcgnwrhvan cpfdrenpad
      241 rrenlprddr ltarvsthal qstrpalrdi kicsreavig aivdtgadit vvreslvpee
      301 fvsphgtidl vsafgekvea klavvplalc rgaplienvd datpvlctlt dkltqadcli
      361 sadawkqlhd sgktdseslv qavgtlsppa edrpdrsgpd vctepvlafa detlpqqtvd
      421 snevepsqag gtaelsgaqk fraeqhedet lrqawqnakq gkagrchttp lachpsecft
      481 aetq
//
LOCUS       XP_077506279             320 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115685 [Amblyomma americanum].
ACCESSION   XP_077506279
VERSION     XP_077506279.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650153.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..320
                     /product="uncharacterized protein LOC144115685"
                     /calculated_mol_wt=34594
     Region          28..115
                     /region_name="MADF_DNA_bdg"
                     /note="Alcohol dehydrogenase transcription factor
                     Myb/SANT-like; pfam10545"
                     /db_xref="CDD:463144"
     CDS             1..320
                     /gene="LOC144115685"
                     /coded_by="XM_077650153.1:126..1088"
                     /db_xref="GeneID:144115685"
ORIGIN      
        1 mdaagsstsg ggqrstnksw tnekearlis lygthrllwd shhpeynnkd srehaikaia
       61 qgldnefnvv svkdkikslr dyfvkelkke avsmnspavd pyvsrwehfr swdflrcvic
      121 cdqsmqsspt vdlpsgplag phcvsdamlp tfvlprasrr tgvkqerqqv ypeffvgeep
      181 dmiipsspel ssdedeerps stettslspp sspvpqascn sglalrdhal lrsqpkteqg
      241 sspsspsypg rdsvtspsvp ppkrarpsts perpqenhrg atpasfsggq pssaesstlp
      301 spvatcaasf lvgsessnpi
//
LOCUS       XP_077506280            1643 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115686 [Amblyomma americanum].
ACCESSION   XP_077506280
VERSION     XP_077506280.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650154.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1643
                     /product="uncharacterized protein LOC144115686"
                     /calculated_mol_wt=177098
     Region          35..131
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          <581..760
                     /region_name="PspC_subgroup_2"
                     /note="pneumococcal surface protein PspC, LPXTG-anchored
                     form; NF033839"
                     /db_xref="CDD:468202"
     Region          784..904
                     /region_name="BTB2_POZ_BTBD8"
                     /note="second BTB (Broad-Complex, Tramtrack and Bric a
                     brac)/POZ (poxvirus and zinc finger) domain found in
                     BTB/POZ domain-containing protein 8 (BTBD8); cd18286"
                     /db_xref="CDD:349595"
     Region          910..974
                     /region_name="BACK_BTBD8"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     BTB/POZ domain-containing protein 8 (BTBD8); cd18490"
                     /db_xref="CDD:350565"
     Region          1195..>1630
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..1643
                     /gene="LOC144115686"
                     /coded_by="XM_077650154.1:148..5079"
                     /db_xref="GeneID:144115686"
ORIGIN      
        1 maagspawsp lhpspgfllk cgkeheklkl riraqlqshm lslledglfs dvtikslsdn
       61 spdglalkah kavllvrvpa fwkllterca isgeganefa vpldkdllrd flrsvysddd
      121 irkkehdilr alrsqdgpas tddedggddd fqdmtgetgt rdgtrevtes gsvnepggdn
      181 dglftgsleq tgaedslenf rscfvndndt nctknhaatt gnvepldslc dnsqvyeesq
      241 acddvsdnrd ecsvsmsssm vrsgtfdlqs qmpleaaiek wngeetaaim gvqeheeqqh
      301 ssksttvdec nkaglkvste qdgkslapmp afflhekspa pmpvsyqclp rspkvggqlg
      361 ekrgscaafg mndsgfmsqn tsvteavayd slndmgevps qgaadqaepg snqerrasga
      421 lfpfyidink trpeenqaqq hrepsnqgss lymyidaggd adagqaqkrs pannpkgdsd
      481 sskrpqscym yvdfntahhd dshpdekpss rtmprtlsls mfidinddhs esvvcaqeay
      541 snyqgkptkd gantverqrs ivehrmwehf ereeilvvse eipameqptr mapcptaegd
      601 vssprrvsac qtrfqppevc llpmspilrr kvkpepvkpa pralpvksst rpqdvdamtf
      661 rrsevcdged sdslehesdr egnvpkkdvl pkaeevpsag pvsstvlpkk pemealvtkv
      721 dtslvqespa vmpretiprp pfspgdgddd setvysevse vsclssidkr mqesgcsper
      781 lgacsklgdd llrmfigqve tditirvegk digahrfila srsdyfslil redfaaeete
      841 fismdgfthg avvfalhhly ggtaspprev hvselallae vlgvdslrrv vlshvrtyyc
      901 hffhkpcsqc iagvaeclqm apccsglrel qaravqwagr hyvrvwphra fatvpehwqq
      961 qcyaatlqal taetaidmal ncerlsvtlp rvrwaesals lanrllqdcv glmsrefdqv
     1021 lqskgfldlg kgqswnital edtllaaveg ltpdaaclsc ialawllped dsqqvqwtqn
     1081 ftdllrkiqr qcerflihna nrvvhcqsws slspqlqkki rdaavivfef ekpiappprl
     1141 sslrrkprrk saggagtada cgdapkdsas psparrprsl argvsssspk tsgsdsselh
     1201 gpaplsaaaa arptaavrra tpgstsgqgv tnkvdskhpa aspsdskavl tgamsesies
     1261 yresssdsgk eqvrsaprve kkmavgarna rarisrgars spnvprdlga rpksvatwaa
     1321 pvshsspngg sitaerssvv gsgspipltv wanpqsrppt qyfqslrqiq skvdsgrplh
     1381 rcsgqvvrvp pftiksdvpt ataatdsgam etdvsrpsdq hhsrqqvgtl gvasnnnnqv
     1441 gnggaigdee edeacdnssa atgepspdas pdfmaeidae snlvshclqe aellerelsr
     1501 klrkqkrgpd pdavpytrpp alrgggtaas vpgaasgnlp qrrraastse akpssggalt
     1561 ahtraaslhg plppsarnss aarssavmtr ssgsggpamv vripgavatp smhlqgravs
     1621 gsesrpphkv sgiavstkpa irr
//
LOCUS       XP_077506281            1643 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115686 [Amblyomma americanum].
ACCESSION   XP_077506281
VERSION     XP_077506281.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650155.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1643
                     /product="uncharacterized protein LOC144115686"
                     /calculated_mol_wt=177098
     Region          35..131
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          <581..760
                     /region_name="PspC_subgroup_2"
                     /note="pneumococcal surface protein PspC, LPXTG-anchored
                     form; NF033839"
                     /db_xref="CDD:468202"
     Region          784..904
                     /region_name="BTB2_POZ_BTBD8"
                     /note="second BTB (Broad-Complex, Tramtrack and Bric a
                     brac)/POZ (poxvirus and zinc finger) domain found in
                     BTB/POZ domain-containing protein 8 (BTBD8); cd18286"
                     /db_xref="CDD:349595"
     Region          910..974
                     /region_name="BACK_BTBD8"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     BTB/POZ domain-containing protein 8 (BTBD8); cd18490"
                     /db_xref="CDD:350565"
     Region          1195..>1630
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..1643
                     /gene="LOC144115686"
                     /coded_by="XM_077650155.1:185..5116"
                     /db_xref="GeneID:144115686"
ORIGIN      
        1 maagspawsp lhpspgfllk cgkeheklkl riraqlqshm lslledglfs dvtikslsdn
       61 spdglalkah kavllvrvpa fwkllterca isgeganefa vpldkdllrd flrsvysddd
      121 irkkehdilr alrsqdgpas tddedggddd fqdmtgetgt rdgtrevtes gsvnepggdn
      181 dglftgsleq tgaedslenf rscfvndndt nctknhaatt gnvepldslc dnsqvyeesq
      241 acddvsdnrd ecsvsmsssm vrsgtfdlqs qmpleaaiek wngeetaaim gvqeheeqqh
      301 ssksttvdec nkaglkvste qdgkslapmp afflhekspa pmpvsyqclp rspkvggqlg
      361 ekrgscaafg mndsgfmsqn tsvteavayd slndmgevps qgaadqaepg snqerrasga
      421 lfpfyidink trpeenqaqq hrepsnqgss lymyidaggd adagqaqkrs pannpkgdsd
      481 sskrpqscym yvdfntahhd dshpdekpss rtmprtlsls mfidinddhs esvvcaqeay
      541 snyqgkptkd gantverqrs ivehrmwehf ereeilvvse eipameqptr mapcptaegd
      601 vssprrvsac qtrfqppevc llpmspilrr kvkpepvkpa pralpvksst rpqdvdamtf
      661 rrsevcdged sdslehesdr egnvpkkdvl pkaeevpsag pvsstvlpkk pemealvtkv
      721 dtslvqespa vmpretiprp pfspgdgddd setvysevse vsclssidkr mqesgcsper
      781 lgacsklgdd llrmfigqve tditirvegk digahrfila srsdyfslil redfaaeete
      841 fismdgfthg avvfalhhly ggtaspprev hvselallae vlgvdslrrv vlshvrtyyc
      901 hffhkpcsqc iagvaeclqm apccsglrel qaravqwagr hyvrvwphra fatvpehwqq
      961 qcyaatlqal taetaidmal ncerlsvtlp rvrwaesals lanrllqdcv glmsrefdqv
     1021 lqskgfldlg kgqswnital edtllaaveg ltpdaaclsc ialawllped dsqqvqwtqn
     1081 ftdllrkiqr qcerflihna nrvvhcqsws slspqlqkki rdaavivfef ekpiappprl
     1141 sslrrkprrk saggagtada cgdapkdsas psparrprsl argvsssspk tsgsdsselh
     1201 gpaplsaaaa arptaavrra tpgstsgqgv tnkvdskhpa aspsdskavl tgamsesies
     1261 yresssdsgk eqvrsaprve kkmavgarna rarisrgars spnvprdlga rpksvatwaa
     1321 pvshsspngg sitaerssvv gsgspipltv wanpqsrppt qyfqslrqiq skvdsgrplh
     1381 rcsgqvvrvp pftiksdvpt ataatdsgam etdvsrpsdq hhsrqqvgtl gvasnnnnqv
     1441 gnggaigdee edeacdnssa atgepspdas pdfmaeidae snlvshclqe aellerelsr
     1501 klrkqkrgpd pdavpytrpp alrgggtaas vpgaasgnlp qrrraastse akpssggalt
     1561 ahtraaslhg plppsarnss aarssavmtr ssgsggpamv vripgavatp smhlqgravs
     1621 gsesrpphkv sgiavstkpa irr
//
LOCUS       XP_077506282             521 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115687 [Amblyomma americanum].
ACCESSION   XP_077506282
VERSION     XP_077506282.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650156.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..521
                     /product="uncharacterized protein LOC144115687"
                     /calculated_mol_wt=55027
     Region          7..144
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(10..11,95..100,111..113,134..137)
                     /site_type="active"
                     /db_xref="CDD:380969"
     Site            order(10..11,96..100,136)
                     /site_type="other"
                     /note="SAM binding site [polypeptide binding]"
                     /db_xref="CDD:380969"
     Region          <147..368
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          462..482
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(462,465,478,482)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:275368"
     Region          499..520
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          500..520
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..521
                     /gene="LOC144115687"
                     /coded_by="XM_077650156.1:463..2028"
                     /db_xref="GeneID:144115687"
ORIGIN      
        1 maalgaadrr lslvaaepli pgdslgpvpp lglagvdpla alawrcldea panaavkavs
       61 awrpdcpeqq pggaaadeer shrqqddeee rlrwlsqlpa arhpqeqnle vtlasqqlql
      121 rvcrpvqrge pllawfsrel adllrlpaap sprnsgnahg gaggekpyrc plcsdafanp
      181 ypvvshlmyr cparhhaasa eaqlpaqppa vprratdatp epaaavepkp ralaalptar
      241 kvkgfdiasl tdccggggae skhqsanard vkmtvdrmar geaatasppa hspaapseya
      301 aaacvpddls fkrpskkrar aeddaavtap pssafkkvdk tdrsppglsp vaggfpfafp
      361 yglppssplc aqfpllpvfg plldpkvedd vvfdknsssa snnssaaaaa aaaaaaaaar
      421 rflpaaaaaa aaaaaaqypa aavlpylpps laalslpsqn wcakcqtsfr mtsdlvyhmr
      481 shhkrepdpa kkrredklrc hichesfrer hhltrhmtsh q
//
LOCUS       XP_077506283             390 aa            linear   INV 25-NOV-2025
DEFINITION  glutamate receptor 2-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077506283
VERSION     XP_077506283.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650157.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..390
                     /product="glutamate receptor 2-like isoform X1"
                     /calculated_mol_wt=43739
     Region          4..253
                     /region_name="Periplasmic_Binding_Protein_Type_2"
                     /note="Type 2 periplasmic binding fold superfamily;
                     cl21456"
                     /db_xref="CDD:473866"
     CDS             1..390
                     /gene="LOC144115688"
                     /coded_by="XM_077650157.1:149..1321"
                     /db_xref="GeneID:144115688"
ORIGIN      
        1 mptdgnwggr lpdgswggqv glvvrnecdv avgplaltnd rvsamqplpa yfyadashma
       61 grrqvfvsdv fgyiyafdeq vwmgiglala avaiimvmfq nnfkfsgagg klfdyvfllf
      121 amlltkgpsr cpentrvlig vwwvavvfat tsftshmqas maiktevarl esvsdilkhr
      181 rivptiidgm afdvffktsa naedqelwrr vqqhrsqlpf rqvfrretye avldgrqvvf
      241 meqslyhygi rrhysqeppr gqfyfgkvnv ltvpcaifvs ynattfvkkt lhratrwice
      301 sglldrhldq vllqtwrqie ssspaveair addisglfav wimgmgmaaa atfaerlaac
      361 qaragprssl rrrrnklkpg lfdclhcvgp
//
LOCUS       XP_077506284             390 aa            linear   INV 25-NOV-2025
DEFINITION  glutamate receptor 2-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077506284
VERSION     XP_077506284.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650158.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..390
                     /product="glutamate receptor 2-like isoform X1"
                     /calculated_mol_wt=43739
     Region          4..253
                     /region_name="Periplasmic_Binding_Protein_Type_2"
                     /note="Type 2 periplasmic binding fold superfamily;
                     cl21456"
                     /db_xref="CDD:473866"
     CDS             1..390
                     /gene="LOC144115688"
                     /coded_by="XM_077650158.1:80..1252"
                     /db_xref="GeneID:144115688"
ORIGIN      
        1 mptdgnwggr lpdgswggqv glvvrnecdv avgplaltnd rvsamqplpa yfyadashma
       61 grrqvfvsdv fgyiyafdeq vwmgiglala avaiimvmfq nnfkfsgagg klfdyvfllf
      121 amlltkgpsr cpentrvlig vwwvavvfat tsftshmqas maiktevarl esvsdilkhr
      181 rivptiidgm afdvffktsa naedqelwrr vqqhrsqlpf rqvfrretye avldgrqvvf
      241 meqslyhygi rrhysqeppr gqfyfgkvnv ltvpcaifvs ynattfvkkt lhratrwice
      301 sglldrhldq vllqtwrqie ssspaveair addisglfav wimgmgmaaa atfaerlaac
      361 qaragprssl rrrrnklkpg lfdclhcvgp
//
LOCUS       XP_077506285             362 aa            linear   INV 25-NOV-2025
DEFINITION  glutamate receptor 4-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077506285
VERSION     XP_077506285.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650159.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..362
                     /product="glutamate receptor 4-like isoform X2"
                     /calculated_mol_wt=40654
     Region          53..>188
                     /region_name="Lig_chan"
                     /note="Ligand-gated ion channel; pfam00060"
                     /db_xref="CDD:459656"
     CDS             1..362
                     /gene="LOC144115688"
                     /coded_by="XM_077650159.1:178..1266"
                     /db_xref="GeneID:144115688"
ORIGIN      
        1 mlamfsvggv tgrpahdvtw sarllaetrv hpplrgkccc llklypttvg skvwmgigla
       61 laavaiimvm fqnnfkfsga ggklfdyvfl lfamlltkgp srcpentrvl igvwwvavvf
      121 attsftshmq asmaikteva rlesvsdilk hrrivptiid gmafdvffkt sanaedqelw
      181 rrvqqhrsql pfrqvfrret yeavldgrqv vfmeqslyhy girrhysqep prgqfyfgkv
      241 nvltvpcaif vsynattfvk ktlhratrwi cesglldrhl dqvllqtwrq iessspavea
      301 iraddisglf avwimgmgma aaatfaerla acqaragprs slrrrrnklk pglfdclhcv
      361 gp
//
LOCUS       XP_077506286             236 aa            linear   INV 25-NOV-2025
DEFINITION  polyisoprenoid diphosphate/phosphate phosphohydrolase PLPP6-like
            isoform X1 [Amblyomma americanum].
ACCESSION   XP_077506286
VERSION     XP_077506286.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650160.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..236
                     /product="polyisoprenoid diphosphate/phosphate
                     phosphohydrolase PLPP6-like isoform X1"
                     /calculated_mol_wt=26339
     Region          49..203
                     /region_name="PAP2_like"
                     /note="PAP2_like proteins, a super-family of histidine
                     phosphatases and vanadium haloperoxidases, includes type 2
                     phosphatidic acid phosphatase or lipid phosphate
                     phosphatase (LPP), Glucose-6-phosphatase,
                     Phosphatidylglycerophosphatase B and bacterial acid...;
                     cl00474"
                     /db_xref="CDD:469784"
     Site            order(115,122,143..145,181,187,191)
                     /site_type="active"
                     /db_xref="CDD:238813"
     CDS             1..236
                     /gene="LOC144115690"
                     /coded_by="XM_077650160.1:291..1001"
                     /db_xref="GeneID:144115690"
ORIGIN      
        1 mkrasagsgr elrsdgdesk elpppgwkrn pillvlrgvd evlsshlfla ahekaplaqy
       61 rhilaafems ahsavwiggl tfllwffafd vpvrtfflnv flallidvvl vavlkavarr
      121 rrpgvsreqy esqplssnls fpsgftsraf lvtlivvkhs hlfplfklpf lvwciavaic
      181 kllmgrqflg dslagavlgy veyhwiaspl wlseetasyf fnsfnaleep tydall
//
LOCUS       XP_077506287             229 aa            linear   INV 25-NOV-2025
DEFINITION  polyisoprenoid diphosphate/phosphate phosphohydrolase PLPP6-like
            isoform X2 [Amblyomma americanum].
ACCESSION   XP_077506287
VERSION     XP_077506287.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650161.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..229
                     /product="polyisoprenoid diphosphate/phosphate
                     phosphohydrolase PLPP6-like isoform X2"
                     /calculated_mol_wt=25260
     Region          49..198
                     /region_name="PAP2_like"
                     /note="PAP2_like proteins, a super-family of histidine
                     phosphatases and vanadium haloperoxidases, includes type 2
                     phosphatidic acid phosphatase or lipid phosphate
                     phosphatase (LPP), Glucose-6-phosphatase,
                     Phosphatidylglycerophosphatase B and bacterial acid...;
                     cl00474"
                     /db_xref="CDD:469784"
     Site            order(115,135..137,173,179,183)
                     /site_type="active"
                     /db_xref="CDD:238813"
     CDS             1..229
                     /gene="LOC144115690"
                     /coded_by="XM_077650161.1:303..992"
                     /db_xref="GeneID:144115690"
ORIGIN      
        1 mkrasagsgr elrsdgdesk elpppgwkrn pillvlrgvd evlsshlfla ahekaplaqy
       61 rhilaafems ahsavwiggl tfllwffafd vpvrtfflnv flavlvdavl mamlkaaasq
      121 rdcdeqqsst lgfpsnftsr avlvtlvvvn rtgafcmlka ffvtwcigva ltktllgkhy
      181 lgdslmgaal gyleyrivve piwcseemvs vlfkafcale epthdsllw
//
LOCUS       XP_077506288             187 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115691 [Amblyomma americanum].
ACCESSION   XP_077506288
VERSION     XP_077506288.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650162.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="uncharacterized protein LOC144115691"
                     /calculated_mol_wt=21216
     CDS             1..187
                     /gene="LOC144115691"
                     /coded_by="XM_077650162.1:3181..3744"
                     /db_xref="GeneID:144115691"
ORIGIN      
        1 mrerrkikar sgaspdelyq pkwahykslm fldtscssqp sfstlhtqee eeeterqceh
       61 wnnesadqem dfpcspqnca epslptqqig egtsrpgkkr rrnddeteqr kvllttavet
      121 lqrsqqrlaa ndecdafgsm mahavrqipp gpdrqlavln ahqaivkinl srhsvpveml
      181 nvlnmqe
//
LOCUS       XP_077506290             334 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial amidoxime-reducing component 1-like [Amblyomma
            americanum].
ACCESSION   XP_077506290
VERSION     XP_077506290.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650164.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..334
                     /product="mitochondrial amidoxime-reducing component
                     1-like"
                     /calculated_mol_wt=37178
     Region          46..324
                     /region_name="YcbX"
                     /note="N-hydroxylaminopurine reductase subunit YcbX,
                     contains MOSC domain [Nucleotide transport and metabolism,
                     Defense mechanisms]; COG3217"
                     /db_xref="CDD:442450"
     CDS             1..334
                     /gene="LOC144115692"
                     /coded_by="XM_077650164.1:1081..2085"
                     /db_xref="GeneID:144115692"
ORIGIN      
        1 masstqtasp lgavalattv vggtiaaayy lwqkhkvaan ntfvpiahlv niviypiksi
       61 agvevpyadc tvagpvykgl kdrqlllvkd dyfvsmreep rlgmirvsfd edactltltv
      121 ngypplvldi sapeeetkps fsvrvrkfsy kavevseeas ewfrnflksd kirlvrilld
      181 qatidrgldg tasvafqdrs sfqvlskasl delvsklppg tdirrrnfrp tlfvegceah
      241 sedhwvryri aeaemafler trrcimttvd qdtgirseke plvtlrtyrv dksklglery
      301 dskpllgigs ihvkdgriki gdevwavvap kpll
//
LOCUS       XP_077506291             411 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115693 [Amblyomma americanum].
ACCESSION   XP_077506291
VERSION     XP_077506291.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650165.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..411
                     /product="uncharacterized protein LOC144115693"
                     /calculated_mol_wt=46257
     CDS             1..411
                     /gene="LOC144115693"
                     /coded_by="XM_077650165.1:25..1260"
                     /db_xref="GeneID:144115693"
ORIGIN      
        1 mlnvqlcaav lfsgsnptat lrmlasigvp vvsnrtffti qrsylwpaid rvwkeeqksl
       61 lqelqgqqvn lagdgrsdsp gfsakygtyt lmdverhkvl hfevvqsnda ggscrmeleg
      121 lkqglafles anikvavlvt drhtqikcfl rnnkaaithe fdvwhmakgi tkklhtaakq
      181 nsckelapwi ksvcnhlywa aassegkaql ivpkwlslln hirdihthdq alfpmclhgd
      241 iepkqwlree seafqkledi akskallqdm prlstrhqty gleafhsllv hftpktyhys
      301 ysgmrartql aalhynensg rgqartkdgt lrwcvrypka aggdptacpv keppthgyvk
      361 clldkvvama estqpqerkt apesrpplss sfpkvpkekl vekrksrfnr q
//
LOCUS       XP_077506292             180 aa            linear   INV 25-NOV-2025
DEFINITION  P2X purinoceptor 7-like [Amblyomma americanum].
ACCESSION   XP_077506292
VERSION     XP_077506292.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650166.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..180
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..180
                     /product="P2X purinoceptor 7-like"
                     /calculated_mol_wt=20013
     Region          <55..175
                     /region_name="P2RX7_C"
                     /note="P2X purinoreceptor 7 intracellular domain;
                     pfam20478"
                     /db_xref="CDD:466627"
     CDS             1..180
                     /gene="LOC144115694"
                     /coded_by="XM_077650166.1:295..837"
                     /db_xref="GeneID:144115694"
ORIGIN      
        1 megfhstvql gtspylfepd aspqgsstap pssdssslaa sesdeaflrv gnvnwcsckh
       61 cavmtekedc lccqevpeit gkqkrarcit ttkkfkdvcl nkavlevala rqesrasrnd
      121 rgsvnkrmry aayrqfvylv wkrlgrhkre ilpscvlsav rkkylsadgs ytgfkhpytc
//
LOCUS       XP_077506293             180 aa            linear   INV 25-NOV-2025
DEFINITION  P2X purinoceptor 7-like [Amblyomma americanum].
ACCESSION   XP_077506293
VERSION     XP_077506293.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650167.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..180
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..180
                     /product="P2X purinoceptor 7-like"
                     /calculated_mol_wt=20013
     Region          <55..175
                     /region_name="P2RX7_C"
                     /note="P2X purinoreceptor 7 intracellular domain;
                     pfam20478"
                     /db_xref="CDD:466627"
     CDS             1..180
                     /gene="LOC144115694"
                     /coded_by="XM_077650167.1:295..837"
                     /db_xref="GeneID:144115694"
ORIGIN      
        1 megfhstvql gtspylfepd aspqgsstap pssdssslaa sesdeaflrv gnvnwcsckh
       61 cavmtekedc lccqevpeit gkqkrarcit ttkkfkdvcl nkavlevala rqesrasrnd
      121 rgsvnkrmry aayrqfvylv wkrlgrhkre ilpscvlsav rkkylsadgs ytgfkhpytc
//
LOCUS       XP_077506294             304 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115695 [Amblyomma americanum].
ACCESSION   XP_077506294
VERSION     XP_077506294.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650168.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..304
                     /product="uncharacterized protein LOC144115695"
                     /calculated_mol_wt=34628
     Region          <49..>141
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..304
                     /gene="LOC144115695"
                     /coded_by="XM_077650168.1:1..915"
                     /db_xref="GeneID:144115695"
ORIGIN      
        1 mtecfdivnq llalsgdvel npgpntrhqv qdslpdvqsk qlqdmfaile ilstrtakmq
       61 ldqkafiksi ddlkksqlkl edlsemnkrl tnverktvti detekevhpf qqlvdqlsse
      121 nsqlrarlse ledrqrrdnl lfygipdaqs eswaqseekl vkvlssclei psseilidta
      181 hrlggftsek crpiivkfss fkvkqrilln csklkddkit lsedfsfatr harkklvefg
      241 ksqpltfklr fnklyiknkc ysynhsddsv heipsafplp tdrsedptnd thsasappte
      301 lvtn
//
LOCUS       XP_077506295             512 aa            linear   INV 25-NOV-2025
DEFINITION  hexokinase-4-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077506295
VERSION     XP_077506295.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650169.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..512
                     /product="hexokinase-4-like isoform X1"
                     /calculated_mol_wt=55990
     Region          36..498
                     /region_name="ASKHA_ATPase-like"
                     /note="ATPase-like domain of the ASKHA (Acetate and Sugar
                     Kinases/Hsc70/Actin) superfamily; cl49607"
                     /db_xref="CDD:483947"
     CDS             1..512
                     /gene="LOC144115698"
                     /coded_by="XM_077650169.1:149..1687"
                     /db_xref="GeneID:144115698"
ORIGIN      
        1 mdpsalekvm dqcypvllvn daekqdvvqq avrpfvldah tvrrvrdtfr reielglqrn
       61 perpsslqmh aahipelpdg tetgdvlald lggtnfrvlh lrlgpgtpse yrvkhyavpe
      121 vvrkgpgegl fdfladclrd fveasglsgr rlplgfcfsf pivqnapdag vliawtvsyd
      181 csgvvgqdvg rmlrdairrp ttvlskvssa imcasprlsq ptscyrrtdl dmnlvaivnd
      241 ttatlvqgaf ldsdcaigli mgtgsnacyl ertenidklh vegedreker tsevvvntew
      301 gafgdngvld fvktefdrtv dhqslftgsf tfekllsgef lgeivrqvlv elgrqrvlfv
      361 ddgavtklkk pgtftavdvs qflqaegvds rrntrdiaar fvasaeritd ddiaiirhvc
      421 gvisaraavf vsvclaelle rvgrstsvti avdgslykhh ptlrtlmdkl italapgrpf
      481 klmlaedgsg kgvglvaavm erlrcpkqng dv
//
LOCUS       XP_077506296             485 aa            linear   INV 25-NOV-2025
DEFINITION  hexokinase-1-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077506296
VERSION     XP_077506296.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650170.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..485
                     /product="hexokinase-1-like isoform X2"
                     /calculated_mol_wt=53124
     Region          36..471
                     /region_name="ASKHA_ATPase-like"
                     /note="ATPase-like domain of the ASKHA (Acetate and Sugar
                     Kinases/Hsc70/Actin) superfamily; cl49607"
                     /db_xref="CDD:483947"
     CDS             1..485
                     /gene="LOC144115698"
                     /coded_by="XM_077650170.1:147..1604"
                     /db_xref="GeneID:144115698"
ORIGIN      
        1 mdpsalekvm dqcypvllvn daekqdvvqq avrpfvldah tvrrvrdtfr reielglqrn
       61 perpsslqmh aahipelpdg tetgdvlald lggtnfrvlh lrlgpgtpse yrvkhyavpe
      121 vvrkgpgegl fdfladclrd fveasglsgr rlplgfcfsf pivqnapdag vliawtvsyd
      181 csgvvgqdvg rmlrdairrr tdldmnlvai vndttatlvq gafldsdcai glimgtgsna
      241 cylertenid klhvegedre kertsevvvn tewgafgdng vldfvktefd rtvdhqslft
      301 gsftfeklls geflgeivrq vlvelgrqrv lfvddgavtk lkkpgtftav dvsqflqaeg
      361 vdsrrntrdi aarfvasaer itdddiaiir hvcgvisara avfvsvclae llervgrsts
      421 vtiavdgsly khhptlrtlm dklitalapg rpfklmlaed gsgkgvglva avmerlrcpk
      481 qngdv
//
LOCUS       XP_077506298             272 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115699 [Amblyomma americanum].
ACCESSION   XP_077506298
VERSION     XP_077506298.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650172.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..272
                     /product="uncharacterized protein LOC144115699"
                     /calculated_mol_wt=28948
     CDS             1..272
                     /gene="LOC144115699"
                     /coded_by="XM_077650172.1:102..920"
                     /db_xref="GeneID:144115699"
ORIGIN      
        1 mtvttteass gadgqkpndg gtdagaatfe teargsddqh thsgdcaeps sdvktpefek
       61 segsqnnepg ysgesprnlp tgnqadaaat avgepckqva rkstaenigg asagplrspa
      121 etnagserfr qkrppslily lrdeescyrr vnsernqqnp ksapevlghr hdsseighsv
      181 sgskdgrddr rgsgrvsgee sagtisdvts eaflasdens sdeplggval rldsfrdnsn
      241 rrfrpssfrp pyffagvslg lvlvyicrry ws
//
LOCUS       XP_077506299             153 aa            linear   INV 25-NOV-2025
DEFINITION  NPC intracellular cholesterol transporter 2-like [Amblyomma
            americanum].
ACCESSION   XP_077506299
VERSION     XP_077506299.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650173.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..153
                     /product="NPC intracellular cholesterol transporter
                     2-like"
                     /calculated_mol_wt=16590
     Region          31..149
                     /region_name="Npc2_like"
                     /note="Niemann-Pick type C2 (Npc2) is a lysosomal protein
                     in which a mutation in the gene causes a rare form of
                     Niemann-Pick type C disease, an autosomal recessive lipid
                     storage disorder characterized by accumulation of
                     low-density lipoprotein-derived...; cd00916"
                     /db_xref="CDD:238458"
     Site            order(89,120,124)
                     /site_type="other"
                     /note="putative cholesterol/lipid binding site [chemical
                     binding]"
                     /db_xref="CDD:238458"
     CDS             1..153
                     /gene="LOC144115700"
                     /coded_by="XM_077650173.1:132..593"
                     /db_xref="GeneID:144115700"
ORIGIN      
        1 madgrcclfa alllvvsagl aaavfdvvqh ekcggefsei ridpcpqlpc hfqkgkplkl
       61 qvdfvaaeni qklemklrge lsngvwlpfp gfrknacknn gltcpleagk sytlestlnv
      121 lssfpsvdan vewsmkgdnk tifcflvpvk vvd
//
LOCUS       XP_077506300             539 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome P450 4C1-like [Amblyomma americanum].
ACCESSION   XP_077506300
VERSION     XP_077506300.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650174.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..539
                     /product="cytochrome P450 4C1-like"
                     /calculated_mol_wt=62563
     Region          94..530
                     /region_name="CYP4"
                     /note="cytochrome P450 family 4; cd20628"
                     /db_xref="CDD:410721"
     Site            order(124,142..143,150,154,161,340,343..344,347,409,413,
                     474..476,480..484,487..488,492)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410721"
     Site            order(143,342..343,347,412,414)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410721"
     CDS             1..539
                     /gene="LOC144115701"
                     /coded_by="XM_077650174.1:56..1675"
                     /db_xref="GeneID:144115701"
ORIGIN      
        1 matsaealgf fvhyvrawvv hgillflasw vfgvvrqwws vrqalkplpg ppdavpfwtm
       61 ltlyrsrrav itqqtagtef syllralcem yknrtfktyl gvspfviiqn pedaevllss
      121 tenlrkplfy sftipwlgpr nmlyiadnvw rykrklqmpa fhsktmekfm avfnehadsm
      181 serleeagnk relapihdit krcaldviae vlmglnlntq knqnsqyney isgitflmav
      241 rafrpwmwfq siynisiegm wfrkmvngvn sfnskimner kktfhlyrnq gdlsgseded
      301 skerelqper rmavidilld khfkdpsytm qeirkdidll lfaghdtsst aiawtlyllg
      361 lhpelqrkaq eemdaifmdd vnrdvtredt drmvfleacf keamrlfppi pfigrvldes
      421 ialdgvvipk gvtvfinifg lhrnsnhfdk peefiparfl dgadkkrhpf afipfsagak
      481 ncigqrfayr eskvilakvl rsftiksswp ldrlklstem vtkvkgglrv wvrrrmldq
//
LOCUS       XP_077506301             554 aa            linear   INV 25-NOV-2025
DEFINITION  cytochrome P450 4V2-like [Amblyomma americanum].
ACCESSION   XP_077506301
VERSION     XP_077506301.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650175.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..554
                     /product="cytochrome P450 4V2-like"
                     /calculated_mol_wt=63933
     Region          98..537
                     /region_name="CYP4"
                     /note="cytochrome P450 family 4; cd20628"
                     /db_xref="CDD:410721"
     Site            order(128,146..147,154,158,165,347,350..351,354,416,420,
                     481..483,487..491,494..495,499)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410721"
     Site            order(147,349..350,354,419,421)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410721"
     CDS             1..554
                     /gene="LOC144115702"
                     /coded_by="XM_077650175.1:592..2256"
                     /db_xref="GeneID:144115702"
ORIGIN      
        1 mfvlwtsesp slssqlgvla rvspllvlla aciflarpvv ewvkiwvalr plpgpwdgip
       61 fwysvkaywn mrkttgvkda tvgfltivnq ltreyqwrtf kvylgpmpvv vvhtpeaaet
      121 llmskenhrk pyvysflsnw lgphnlvtat gdiwrfkrrl ftpafhfrvl enyichfnen
      181 gnvlikilek hidekpdeal aafplmqhlt ldiigrvsmg tvlgmqtdtg npfgknlnrl
      241 sfliavrgfr pwtwiqqiyd ltyegkmfke tlldmekfsl svmqkrkekl qqmelnedrg
      301 dededkdceg gnqpigkesi ildyllrkhl eessytidev kkdidtiifg gndtttstts
      361 walyllglhp ekqakvhael devfgsdcdr dvtkadvyrl kylecciket lrlypsipli
      421 grhleedlvi dgyripkgvn cfinlfslhk npkyfkdpes fiperfltee fttrhpfsyl
      481 pfsggpknci gqrfallesk vimaklmlkf svestrpldq lrvsyevivk argglriwfr
      541 rrppfndgwq aakv
//
LOCUS       XP_077506302             465 aa            linear   INV 25-NOV-2025
DEFINITION  bone morphogenetic protein 2-like [Amblyomma americanum].
ACCESSION   XP_077506302
VERSION     XP_077506302.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650176.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..465
                     /product="bone morphogenetic protein 2-like"
                     /calculated_mol_wt=51159
     Region          63..284
                     /region_name="TGFb_propeptide"
                     /note="TGF-beta propeptide; cl46657"
                     /db_xref="CDD:480997"
     Region          363..465
                     /region_name="TGF_beta_ADMP"
                     /note="transforming growth factor beta (TGF-beta) like
                     domain found in anti-dorsalizing morphogenetic protein
                     (ADMP) and similar proteins; cd13765"
                     /db_xref="CDD:381643"
     Site            order(368,370,374..375,377,387,389..391,408..409,412..413,
                     415..416,419,424..426,429,431..432,435,455..457,459,465)
                     /site_type="other"
                     /note="putative homodimer interface [polypeptide binding]"
                     /db_xref="CDD:381643"
     CDS             1..465
                     /gene="LOC144115703"
                     /coded_by="XM_077650176.1:950..2347"
                     /db_xref="GeneID:144115703"
ORIGIN      
        1 mrlawlllla lralsaaavp qrhelsnaie dvssassvsa gseeqprgrq qqprapkdga
       61 avsrllqvfg lreprrrerh qqppvymlel ynatagsggi trsankynan lvrsfpdrvr
      121 rhnkrgkkrs kdkkatrgsg gsakesplrf ffnvtpsesd kedvlqaefh lyklrpkhrt
      181 pdrlgdaksh llevrvyqam pgvsplreeg hrlldvrrmt ytsvgwevfy vkaaavdwik
      241 npasnlgllm tvrtahgrrl eegvlrfakr hqghvnkqpi lvvfsddgrp sretlyptaa
      301 sgdaaggslp tqaigarlpv svphndgptp apsprnvrav pqasssggks ssgakvhdep
      361 fgctrhemyv dfekigwssw iispkgynay hckgqcyfpl gqnqlptnha tvqsivnels
      421 ltagvgapcc vpnklysisl lyfdenenvv lkqyddmvaa scgch
//
LOCUS       XP_077506303             115 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115706 [Amblyomma americanum].
ACCESSION   XP_077506303
VERSION     XP_077506303.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650177.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..115
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..115
                     /product="uncharacterized protein LOC144115706"
                     /calculated_mol_wt=12140
     CDS             1..115
                     /gene="LOC144115706"
                     /coded_by="XM_077650177.1:485..832"
                     /db_xref="GeneID:144115706"
ORIGIN      
        1 mfslesnnmw lvnsqhaaeg gleglpttph lavigssmgd ikkiivavds trslekamgl
       61 llgaffvfnv ayppdcpltm ellqrylgqs nptkgrgkgr acgiapklls llnal
//
LOCUS       XP_077506304             385 aa            linear   INV 25-NOV-2025
DEFINITION  methionine aminopeptidase 1 [Amblyomma americanum].
ACCESSION   XP_077506304
VERSION     XP_077506304.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650178.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..385
                     /product="methionine aminopeptidase 1"
                     /calculated_mol_wt=42772
     Region          21..381
                     /region_name="APP_MetAP"
                     /note="A family including aminopeptidase P, aminopeptidase
                     M, and prolidase. Also known as metallopeptidase family
                     M24. This family of enzymes is able to cleave amido-,
                     imido- and amidino-containing bonds. Members exibit
                     relatively narrow substrate specificity...; cl00279"
                     /db_xref="CDD:469704"
     Site            order(202,219,230,293,326,357)
                     /site_type="active"
                     /db_xref="CDD:238519"
     CDS             1..385
                     /gene="LOC144115707"
                     /coded_by="XM_077650178.1:19..1176"
                     /db_xref="GeneID:144115707"
ORIGIN      
        1 maavdmegaq gemlqnicgt agcksfaklq cptciklgik gsyfcsqdcf kgnwdshkqv
       61 hrvvknakaa anavynpwps fmfsgklrpy plspkrevpe himrpdyaeh pegiplseqa
      121 akhssvikvl ddeeiegvrl asklarevld valgaaeigv ttdeldrlvh easierdcyp
      181 splnyykfpk scctsvnevi chgipdmrpl qdgdllnvdi tvyhrgfhgd lnetvfignv
      241 nesgrklvqv theclskaie avtpgmryre igniiqkhaq shgfsvvrsy cghgihrlfh
      301 tapsvphyak nkaigvmkag hcftiepmis egtwhdevwp dnwtavtadg krsaqfeqtl
      361 lvtdtgceil trrrshdgqp wfmdk
//
LOCUS       XP_077506306             642 aa            linear   INV 25-NOV-2025
DEFINITION  fatty acid transport protein 1 [Amblyomma americanum].
ACCESSION   XP_077506306
VERSION     XP_077506306.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650180.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..642
                     /product="fatty acid transport protein 1"
                     /calculated_mol_wt=71754
     Region          99..606
                     /region_name="AFD_class_I"
                     /note="Adenylate forming domain, Class I superfamily;
                     cl17068"
                     /db_xref="CDD:473059"
     Site            order(242,245..250,252..253)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341228"
     Site            order(245,360..361,381..386,488,500,503,514,594)
                     /site_type="other"
                     /note="AMP binding site [chemical binding]"
                     /db_xref="CDD:341228"
     Site            order(245,285..286,342,344..345,348,360..361,381..386,488,
                     500,503,511..514,575)
                     /site_type="active"
                     /db_xref="CDD:341228"
     Site            order(285,344..345,348,360,511..513,566,575)
                     /site_type="other"
                     /note="CoA binding site [chemical binding]"
                     /db_xref="CDD:341228"
     CDS             1..642
                     /gene="Fatp1"
                     /coded_by="XM_077650180.1:163..2091"
                     /db_xref="GeneID:144115708"
ORIGIN      
        1 mlklaglcgs fsvltlgslc svwmagfitf flyvitggwr flrivfytar rdltglirit
       61 ktslyfkscl kanrtvpmvf qsvvnknmdk vcfimehnrw tfkqvddftn rvancflqqg
      121 lrpgdevavf mdsrpefvmf wlgmskvgiv aalvntnlks dpllhsltci nakaivfgke
      181 qanamkdvap slmekgdyqy yvygacdtqp lpavdieeli knsssvpadi dykgsihdkl
      241 vyiytsgttg lpkaaiikhs rylsmvsask ymmpitaddi lytalplyht aggilavgqa
      301 llfgntvair skfsasrfwd dcikydctvt qyigeicryl laqpvrpqer qhkirmmfgn
      361 glrpqiwsqf qerfgirdir elygstegna hvlnidnkvg svgfvsrias nvhpvklirv
      421 deatgepird knglcvpcep devgelvgri vrddhihsfd gyankaatsk kvykdvfkkg
      481 dmafasgdll vmdefgylff kdrtgdtfrw kgenvstsev egvvariagl tdcaiygvei
      541 pgsegkagma aicdpeskld lkaflrdvrn alplyavplf irvvkdleat gtykikkvdl
      601 qkqgydtdvi ddpmyfldlt aneyvpltkt iyekiqrgea rv
//
LOCUS       XP_077506307             243 aa            linear   INV 25-NOV-2025
DEFINITION  vacuolar-sorting protein SNF8 [Amblyomma americanum].
ACCESSION   XP_077506307
VERSION     XP_077506307.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650181.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..243
                     /product="vacuolar-sorting protein SNF8"
                     /calculated_mol_wt=27405
     Region          6..227
                     /region_name="EAP30"
                     /note="EAP30/Vps36 family; pfam04157"
                     /db_xref="CDD:461201"
     CDS             1..243
                     /gene="lsn"
                     /coded_by="XM_077650181.1:155..886"
                     /db_xref="GeneID:144115709"
ORIGIN      
        1 mrrrvggvga iqkqqlaqar fkdrgselae eqlqqmtrqm eafraklqcf aashkndirk
       61 npqfrrqfqe mcatagvdpl asskgfwadm lgvgdfyyel gvqiievcla tshrngglms
      121 leelrervtk srssahrqee itqddllrav eklktlgkgf qlisvgsryl vqsvptelsl
      181 dhtnvikhag stgyvsvesl vqelgwdsdr aqkaledllk eglvwldaqa qkeylywfpg
      241 lir
//
LOCUS       XP_077506308            1014 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein C05D11.1-like [Amblyomma americanum].
ACCESSION   XP_077506308
VERSION     XP_077506308.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650182.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1014
                     /product="uncharacterized protein C05D11.1-like"
                     /calculated_mol_wt=113867
     Region          45..967
                     /region_name="Cym1"
                     /note="Zn-dependent peptidase, M16 (insulinase) family
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG1026"
                     /db_xref="CDD:440649"
     CDS             1..1014
                     /gene="LOC144115710"
                     /coded_by="XM_077650182.1:278..3322"
                     /db_xref="GeneID:144115710"
ORIGIN      
        1 msnfqeicav rtdgelplak yrstktgmtv cvsefdgpli sgylciatea qdddglphtl
       61 ehlifmgsed ypykgvldql anrclasgtn awtdtdhtcy tmttagskgf lnllpiyldh
      121 ilypllteaa fitevhhvng egdnagvvyc emqarentge slcmlnllrt lypapcgyry
      181 etgglmrnlr estnnqkvra yhknfyrpen lcvivagsvs padvlkalep ferkilskgp
      241 rkpfsrpwqk avppisgsle tvvlypcden dhglvylafr gpplkawedi aallvmqdyl
      301 tdtsvsplqk lfvetddpfc smvkasfien kescfyfgfe nvarlklrmi ktalmkavtd
      361 ivngtvaldm ermtvviqrr rlrilnqves sphesvansv imdflygdti edlrnrvnqi
      421 pvfmgllkrd kqfwlqllkk yivdnkaicv igepspemlk eltdaqnlrv qrqreilgee
      481 glakraeale kaieanerep pkemilavqv ppistisfhs lqracnaqse kgilsfglng
      541 ipfrfeldhi ktnfvtmtvi lntsgisqdl rmhvplflel lhespvmtkg grmnhndvin
      601 kleadtihia tglglenstr ffagshaqca clrllvdqek yfhgvqwvhd lltgiefepd
      661 riqvvankmg kdvvkkkrsg ikitgtlirt iayqpesnqa agsmlrqqtf lcdlmrqlkv
      721 dstkvvkkll evkefltrpe nvtvhlaani eklsligdlr lpwttclsqq pklaspikss
      781 pqvpchslll rgntpglprd vitsvgsves afltqctpsl nsytspdlpp llvliqylvq
      841 legpmwrqir gqglsynydl hirpcdglly fvltkstlvs aayreamqiv qrhvageeew
      901 deelveasrs slmfeiieke kyvnevalqs mlayhrgidm sytrtllekv atvkladmvr
      961 vgkqylaslf npatsklvic chpskvddiv aafkefqrnl tvvtslddsv faky
//
LOCUS       XP_077506309             433 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115711 [Amblyomma americanum].
ACCESSION   XP_077506309
VERSION     XP_077506309.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650183.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..433
                     /product="uncharacterized protein LOC144115711"
                     /calculated_mol_wt=48416
     Region          82..>123
                     /region_name="FH_FOX"
                     /note="Forkhead (FH) domain found in Forkhead box (FOX)
                     family of transcription factors and similar proteins;
                     cl00061"
                     /db_xref="CDD:469596"
     CDS             1..433
                     /gene="LOC144115711"
                     /coded_by="XM_077650183.1:491..1792"
                     /db_xref="GeneID:144115711"
ORIGIN      
        1 mpaipsfapl vphvspgngr pypaveqasr evwhveeass epakrsrhtr tmsshgdtct
       61 mpqlhpgpla ellpagtivr rppaklvqlv aqaihespik alrvrhvyea lkkrypyfmf
      121 mdkkeqttwe gsesrpssrl ssssqsllvh tplpsfigsp grqlghmepq lhplvewelk
      181 terfatppsp siylnteqer kpkpvyswsr gcydlyghqs qgysdevsta tdtdllnepl
      241 pslpylcspr lthdtpvept srgyghpsph qepqlpltle qhsrpelsfa rqqvqpeqea
      301 mmpqqqflpr pelsnsregt lphpelplpp qfpwppqgps lprgpilppr qeetrpwgtg
      361 pgfwprsgql qlpgwpyage pvfaaiantr ravmtddgtw yilvypafpe ndecsaqavw
      421 hweegitagg ntp
//
LOCUS       XP_077506310            1091 aa            linear   INV 25-NOV-2025
DEFINITION  FK506-binding protein 15-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077506310
VERSION     XP_077506310.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650184.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1091
                     /product="FK506-binding protein 15-like isoform X1"
                     /calculated_mol_wt=116855
     Region          <98..156
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          186..276
                     /region_name="FkpA"
                     /note="FKBP-type peptidyl-prolyl cis-trans isomerase
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG0545"
                     /db_xref="CDD:440311"
     Region          <269..453
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          528..>816
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1091
                     /gene="LOC144115712"
                     /coded_by="XM_077650184.1:179..3454"
                     /db_xref="GeneID:144115712"
ORIGIN      
        1 mamlfanddd dadftvqpgg sklaslfsdn eaakagnssl tytppkqpsv gksfdkqpss
       61 gtlsllhaga vqafkyegkk psnvgrlgva vlgnhekrty klilykekqq qvavaainaq
      121 fvirlfgnnf lsfadekrcn wslkfgseea visfakqavi akalslggsl telnyyicqd
      181 lvvpgegdgd slvhgssavi kytawlltse hslkkvfdtt esdepqkiki grgkhvkgle
      241 egllgckkns rrllvlppsf gygskglgtt ippdsvlvyd vsvssvskpk paakdstrrs
      301 stpvsaaavd adkrearsss hgssadesar srapsappdw ppqqpataps lapvaaasgg
      361 esdkarllsr makigaqmlp mpgavpaqps sdsevederl psiqvpttvp lpqpavregs
      421 pkpvvkprsf sqqssgdtvl qptpmqpalq pmasaytpgl ppaaaampaa qhmslyqaqt
      481 qlaqgalagy pyqqqgillp pphaypaapv ptsvstalda qlpvllsetr sqstevrlni
      541 skltdkvdyi igkldtlksq ppsapplssy mdseallhni qrivqenvkl knesseknnk
      601 iqalnekicd llqrnqrlme esntlfeqrs dtlhssaahs qarllaleae kmevseqlnr
      661 tlselsllka elserqhkes qaqkmlgelq svlqdnnnml ksledslkea eqekqdlekq
      721 iegykdaasv kenaahsseq iaqleenisa hwksmcdtet kklsarisal eaekealsar
      781 vmeldskcke latsgekqrq ayedkmrqlq qenqalstga aaasaafdfd telkkimntl
      841 fkllqrefsl eetysskeak givlnsirdy ttavlerrqa satlgngtsr asptstssns
      901 stagppaaaa aaaaaaasqp qqssggggnq egkhqnmqes petqpavekl sssppsgpsa
      961 psqhdecaep tpedsrteas snevvqaske gepnlpqepq dvaptgdeka aakegasgda
     1021 etsspppesk teaepqaesk naqppdwesf laslrqasrv ygqnipplwm tllslavlfv
     1081 sllalrygly c
//
LOCUS       XP_077506311            1090 aa            linear   INV 25-NOV-2025
DEFINITION  FK506-binding protein 15-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077506311
VERSION     XP_077506311.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650185.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1090
                     /product="FK506-binding protein 15-like isoform X2"
                     /calculated_mol_wt=116768
     Region          <98..156
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          186..276
                     /region_name="FkpA"
                     /note="FKBP-type peptidyl-prolyl cis-trans isomerase
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG0545"
                     /db_xref="CDD:440311"
     Region          <257..452
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          527..>815
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1090
                     /gene="LOC144115712"
                     /coded_by="XM_077650185.1:179..3451"
                     /db_xref="GeneID:144115712"
ORIGIN      
        1 mamlfanddd dadftvqpgg sklaslfsdn eaakagnssl tytppkqpsv gksfdkqpss
       61 gtlsllhaga vqafkyegkk psnvgrlgva vlgnhekrty klilykekqq qvavaainaq
      121 fvirlfgnnf lsfadekrcn wslkfgseea visfakqavi akalslggsl telnyyicqd
      181 lvvpgegdgd slvhgssavi kytawlltse hslkkvfdtt esdepqkiki grgkhvkgle
      241 egllgckkns rrllvlppsf gygskglgtt ippdsvlvyd vsvssvskpk paakdstrrs
      301 stpvsaaavd adkrearsss hgsadesars rapsappdwp pqqpatapsl apvaaasgge
      361 sdkarllsrm akigaqmlpm pgavpaqpss dsevederlp siqvpttvpl pqpavregsp
      421 kpvvkprsfs qqssgdtvlq ptpmqpalqp masaytpglp paaaampaaq hmslyqaqtq
      481 laqgalagyp yqqqgillpp phaypaapvp tsvstaldaq lpvllsetrs qstevrlnis
      541 kltdkvdyii gkldtlksqp psapplssym dseallhniq rivqenvklk nesseknnki
      601 qalnekicdl lqrnqrlmee sntlfeqrsd tlhssaahsq arllaleaek mevseqlnrt
      661 lselsllkae lserqhkesq aqkmlgelqs vlqdnnnmlk sledslkeae qekqdlekqi
      721 egykdaasvk enaahsseqi aqleenisah wksmcdtetk klsarisale aekealsarv
      781 meldskckel atsgekqrqa yedkmrqlqq enqalstgaa aasaafdfdt elkkimntlf
      841 kllqrefsle etysskeakg ivlnsirdyt tavlerrqas atlgngtsra sptstssnss
      901 tagppaaaaa aaaaaasqpq qssggggnqe gkhqnmqesp etqpavekls ssppsgpsap
      961 sqhdecaept pedsrteass nevvqaskeg epnlpqepqd vaptgdekaa akegasgdae
     1021 tsspppeskt eaepqaeskn aqppdwesfl aslrqasrvy gqnipplwmt llslavlfvs
     1081 llalryglyc
//
LOCUS       XP_077506312            1090 aa            linear   INV 25-NOV-2025
DEFINITION  FK506-binding protein 15-like isoform X3 [Amblyomma americanum].
ACCESSION   XP_077506312
VERSION     XP_077506312.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650186.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1090
                     /product="FK506-binding protein 15-like isoform X3"
                     /calculated_mol_wt=116727
     Region          <98..156
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          186..276
                     /region_name="FkpA"
                     /note="FKBP-type peptidyl-prolyl cis-trans isomerase
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG0545"
                     /db_xref="CDD:440311"
     Region          <269..453
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          527..>815
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1090
                     /gene="LOC144115712"
                     /coded_by="XM_077650186.1:179..3451"
                     /db_xref="GeneID:144115712"
ORIGIN      
        1 mamlfanddd dadftvqpgg sklaslfsdn eaakagnssl tytppkqpsv gksfdkqpss
       61 gtlsllhaga vqafkyegkk psnvgrlgva vlgnhekrty klilykekqq qvavaainaq
      121 fvirlfgnnf lsfadekrcn wslkfgseea visfakqavi akalslggsl telnyyicqd
      181 lvvpgegdgd slvhgssavi kytawlltse hslkkvfdtt esdepqkiki grgkhvkgle
      241 egllgckkns rrllvlppsf gygskglgtt ippdsvlvyd vsvssvskpk paakdstrrs
      301 stpvsaaavd adkrearsss hgssadesar srapsappdw ppqqpataps lapvaaasgg
      361 esdkarllsr makigaqmlp mpgavpaqps sdsevederl psiqvpttvp lpqpavregs
      421 pkpvvkprsf sqqssgdtvl qptpmqpalq pmasaytpgl ppaaaampaa qhmslyqaqt
      481 qlagalagyp yqqqgillpp phaypaapvp tsvstaldaq lpvllsetrs qstevrlnis
      541 kltdkvdyii gkldtlksqp psapplssym dseallhniq rivqenvklk nesseknnki
      601 qalnekicdl lqrnqrlmee sntlfeqrsd tlhssaahsq arllaleaek mevseqlnrt
      661 lselsllkae lserqhkesq aqkmlgelqs vlqdnnnmlk sledslkeae qekqdlekqi
      721 egykdaasvk enaahsseqi aqleenisah wksmcdtetk klsarisale aekealsarv
      781 meldskckel atsgekqrqa yedkmrqlqq enqalstgaa aasaafdfdt elkkimntlf
      841 kllqrefsle etysskeakg ivlnsirdyt tavlerrqas atlgngtsra sptstssnss
      901 tagppaaaaa aaaaaasqpq qssggggnqe gkhqnmqesp etqpavekls ssppsgpsap
      961 sqhdecaept pedsrteass nevvqaskeg epnlpqepqd vaptgdekaa akegasgdae
     1021 tsspppeskt eaepqaeskn aqppdwesfl aslrqasrvy gqnipplwmt llslavlfvs
     1081 llalryglyc
//
LOCUS       XP_077506313            1089 aa            linear   INV 25-NOV-2025
DEFINITION  FK506-binding protein 15-like isoform X4 [Amblyomma americanum].
ACCESSION   XP_077506313
VERSION     XP_077506313.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650187.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1089
                     /product="FK506-binding protein 15-like isoform X4"
                     /calculated_mol_wt=116640
     Region          <98..156
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          186..276
                     /region_name="FkpA"
                     /note="FKBP-type peptidyl-prolyl cis-trans isomerase
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG0545"
                     /db_xref="CDD:440311"
     Region          <257..452
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          526..>814
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1089
                     /gene="LOC144115712"
                     /coded_by="XM_077650187.1:179..3448"
                     /db_xref="GeneID:144115712"
ORIGIN      
        1 mamlfanddd dadftvqpgg sklaslfsdn eaakagnssl tytppkqpsv gksfdkqpss
       61 gtlsllhaga vqafkyegkk psnvgrlgva vlgnhekrty klilykekqq qvavaainaq
      121 fvirlfgnnf lsfadekrcn wslkfgseea visfakqavi akalslggsl telnyyicqd
      181 lvvpgegdgd slvhgssavi kytawlltse hslkkvfdtt esdepqkiki grgkhvkgle
      241 egllgckkns rrllvlppsf gygskglgtt ippdsvlvyd vsvssvskpk paakdstrrs
      301 stpvsaaavd adkrearsss hgsadesars rapsappdwp pqqpatapsl apvaaasgge
      361 sdkarllsrm akigaqmlpm pgavpaqpss dsevederlp siqvpttvpl pqpavregsp
      421 kpvvkprsfs qqssgdtvlq ptpmqpalqp masaytpglp paaaampaaq hmslyqaqtq
      481 lagalagypy qqqgillppp haypaapvpt svstaldaql pvllsetrsq stevrlnisk
      541 ltdkvdyiig kldtlksqpp sapplssymd seallhniqr ivqenvklkn esseknnkiq
      601 alnekicdll qrnqrlmees ntlfeqrsdt lhssaahsqa rllaleaekm evseqlnrtl
      661 selsllkael serqhkesqa qkmlgelqsv lqdnnnmlks ledslkeaeq ekqdlekqie
      721 gykdaasvke naahsseqia qleenisahw ksmcdtetkk lsarisalea ekealsarvm
      781 eldskckela tsgekqrqay edkmrqlqqe nqalstgaaa asaafdfdte lkkimntlfk
      841 llqrefslee tysskeakgi vlnsirdytt avlerrqasa tlgngtsras ptstssnsst
      901 agppaaaaaa aaaaasqpqq ssggggnqeg khqnmqespe tqpaveklss sppsgpsaps
      961 qhdecaeptp edsrteassn evvqaskege pnlpqepqdv aptgdekaaa kegasgdaet
     1021 sspppeskte aepqaeskna qppdwesfla slrqasrvyg qnipplwmtl lslavlfvsl
     1081 lalryglyc
//
LOCUS       XP_077506314            1069 aa            linear   INV 25-NOV-2025
DEFINITION  FK506-binding protein 15-like isoform X5 [Amblyomma americanum].
ACCESSION   XP_077506314
VERSION     XP_077506314.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650188.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1069
                     /product="FK506-binding protein 15-like isoform X5"
                     /calculated_mol_wt=114566
     Region          <98..156
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          186..276
                     /region_name="FkpA"
                     /note="FKBP-type peptidyl-prolyl cis-trans isomerase
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG0545"
                     /db_xref="CDD:440311"
     Region          <269..453
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          528..>816
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1069
                     /gene="LOC144115712"
                     /coded_by="XM_077650188.1:179..3388"
                     /db_xref="GeneID:144115712"
ORIGIN      
        1 mamlfanddd dadftvqpgg sklaslfsdn eaakagnssl tytppkqpsv gksfdkqpss
       61 gtlsllhaga vqafkyegkk psnvgrlgva vlgnhekrty klilykekqq qvavaainaq
      121 fvirlfgnnf lsfadekrcn wslkfgseea visfakqavi akalslggsl telnyyicqd
      181 lvvpgegdgd slvhgssavi kytawlltse hslkkvfdtt esdepqkiki grgkhvkgle
      241 egllgckkns rrllvlppsf gygskglgtt ippdsvlvyd vsvssvskpk paakdstrrs
      301 stpvsaaavd adkrearsss hgssadesar srapsappdw ppqqpataps lapvaaasgg
      361 esdkarllsr makigaqmlp mpgavpaqps sdsevederl psiqvpttvp lpqpavregs
      421 pkpvvkprsf sqqssgdtvl qptpmqpalq pmasaytpgl ppaaaampaa qhmslyqaqt
      481 qlaqgalagy pyqqqgillp pphaypaapv ptsvstalda qlpvllsetr sqstevrlni
      541 skltdkvdyi igkldtlksq ppsapplssy mdseallhni qrivqenvkl knesseknnk
      601 iqalnekicd llqrnqrlme esntlfeqrs dtlhssaahs qarllaleae kmevseqlnr
      661 tlselsllka elserqhkes qaqkmlgelq svlqdnnnml ksledslkea eqekqdlekq
      721 iegykdaasv kenaahsseq iaqleenisa hwksmcdtet kklsarisal eaekealsar
      781 vmeldskcke latsgekqrq ayedkmrqlq qenqalstga aaasaafdfd telkkimntl
      841 fkllqrefsl eetysskeak givlnsirdy ttavlerrqa satlgngtsr asptstssns
      901 stagppaaaa aaaaaaasqp qqssggggnq egkhqnmqes petqpavekl sssppsgpsa
      961 psqhdecaep tpedsrteas snevvqaske gepnlpqepq dvaptgdeka aakegasgda
     1021 etsspppesk teaepqaesk naqppdwswk pqppppplfd eyedddwln
//
LOCUS       XP_077506315            1068 aa            linear   INV 25-NOV-2025
DEFINITION  FK506-binding protein 15-like isoform X6 [Amblyomma americanum].
ACCESSION   XP_077506315
VERSION     XP_077506315.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650189.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1068
                     /product="FK506-binding protein 15-like isoform X6"
                     /calculated_mol_wt=114479
     Region          <98..156
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          186..276
                     /region_name="FkpA"
                     /note="FKBP-type peptidyl-prolyl cis-trans isomerase
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG0545"
                     /db_xref="CDD:440311"
     Region          <257..452
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          527..>815
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1068
                     /gene="LOC144115712"
                     /coded_by="XM_077650189.1:179..3385"
                     /db_xref="GeneID:144115712"
ORIGIN      
        1 mamlfanddd dadftvqpgg sklaslfsdn eaakagnssl tytppkqpsv gksfdkqpss
       61 gtlsllhaga vqafkyegkk psnvgrlgva vlgnhekrty klilykekqq qvavaainaq
      121 fvirlfgnnf lsfadekrcn wslkfgseea visfakqavi akalslggsl telnyyicqd
      181 lvvpgegdgd slvhgssavi kytawlltse hslkkvfdtt esdepqkiki grgkhvkgle
      241 egllgckkns rrllvlppsf gygskglgtt ippdsvlvyd vsvssvskpk paakdstrrs
      301 stpvsaaavd adkrearsss hgsadesars rapsappdwp pqqpatapsl apvaaasgge
      361 sdkarllsrm akigaqmlpm pgavpaqpss dsevederlp siqvpttvpl pqpavregsp
      421 kpvvkprsfs qqssgdtvlq ptpmqpalqp masaytpglp paaaampaaq hmslyqaqtq
      481 laqgalagyp yqqqgillpp phaypaapvp tsvstaldaq lpvllsetrs qstevrlnis
      541 kltdkvdyii gkldtlksqp psapplssym dseallhniq rivqenvklk nesseknnki
      601 qalnekicdl lqrnqrlmee sntlfeqrsd tlhssaahsq arllaleaek mevseqlnrt
      661 lselsllkae lserqhkesq aqkmlgelqs vlqdnnnmlk sledslkeae qekqdlekqi
      721 egykdaasvk enaahsseqi aqleenisah wksmcdtetk klsarisale aekealsarv
      781 meldskckel atsgekqrqa yedkmrqlqq enqalstgaa aasaafdfdt elkkimntlf
      841 kllqrefsle etysskeakg ivlnsirdyt tavlerrqas atlgngtsra sptstssnss
      901 tagppaaaaa aaaaaasqpq qssggggnqe gkhqnmqesp etqpavekls ssppsgpsap
      961 sqhdecaept pedsrteass nevvqaskeg epnlpqepqd vaptgdekaa akegasgdae
     1021 tsspppeskt eaepqaeskn aqppdwswkp qppppplfde yedddwln
//
LOCUS       XP_077506316            1067 aa            linear   INV 25-NOV-2025
DEFINITION  FK506-binding protein 15-like isoform X7 [Amblyomma americanum].
ACCESSION   XP_077506316
VERSION     XP_077506316.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650190.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1067
                     /product="FK506-binding protein 15-like isoform X7"
                     /calculated_mol_wt=114351
     Region          <98..156
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          186..276
                     /region_name="FkpA"
                     /note="FKBP-type peptidyl-prolyl cis-trans isomerase
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG0545"
                     /db_xref="CDD:440311"
     Region          <257..452
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          526..>814
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1067
                     /gene="LOC144115712"
                     /coded_by="XM_077650190.1:179..3382"
                     /db_xref="GeneID:144115712"
ORIGIN      
        1 mamlfanddd dadftvqpgg sklaslfsdn eaakagnssl tytppkqpsv gksfdkqpss
       61 gtlsllhaga vqafkyegkk psnvgrlgva vlgnhekrty klilykekqq qvavaainaq
      121 fvirlfgnnf lsfadekrcn wslkfgseea visfakqavi akalslggsl telnyyicqd
      181 lvvpgegdgd slvhgssavi kytawlltse hslkkvfdtt esdepqkiki grgkhvkgle
      241 egllgckkns rrllvlppsf gygskglgtt ippdsvlvyd vsvssvskpk paakdstrrs
      301 stpvsaaavd adkrearsss hgsadesars rapsappdwp pqqpatapsl apvaaasgge
      361 sdkarllsrm akigaqmlpm pgavpaqpss dsevederlp siqvpttvpl pqpavregsp
      421 kpvvkprsfs qqssgdtvlq ptpmqpalqp masaytpglp paaaampaaq hmslyqaqtq
      481 lagalagypy qqqgillppp haypaapvpt svstaldaql pvllsetrsq stevrlnisk
      541 ltdkvdyiig kldtlksqpp sapplssymd seallhniqr ivqenvklkn esseknnkiq
      601 alnekicdll qrnqrlmees ntlfeqrsdt lhssaahsqa rllaleaekm evseqlnrtl
      661 selsllkael serqhkesqa qkmlgelqsv lqdnnnmlks ledslkeaeq ekqdlekqie
      721 gykdaasvke naahsseqia qleenisahw ksmcdtetkk lsarisalea ekealsarvm
      781 eldskckela tsgekqrqay edkmrqlqqe nqalstgaaa asaafdfdte lkkimntlfk
      841 llqrefslee tysskeakgi vlnsirdytt avlerrqasa tlgngtsras ptstssnsst
      901 agppaaaaaa aaaaasqpqq ssggggnqeg khqnmqespe tqpaveklss sppsgpsaps
      961 qhdecaeptp edsrteassn evvqaskege pnlpqepqdv aptgdekaaa kegasgdaet
     1021 sspppeskte aepqaeskna qppdwswkpq ppppplfdey edddwln
//
LOCUS       XP_077506317             236 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115714, partial [Amblyomma
            americanum].
ACCESSION   XP_077506317
VERSION     XP_077506317.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650191.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         <1..236
                     /product="uncharacterized protein LOC144115714"
     CDS             1..236
                     /gene="LOC144115714"
                     /coded_by="XM_077650191.1:<1..711"
                     /db_xref="GeneID:144115714"
ORIGIN      
        1 ilclydvlhf sigvfassgn vksqmlskiv leatvlceqa gllvhyvctd gatwnrsmwh
       61 nlgvygngkn vkgkvthpcd edrflhfisd fphlakcvrn tllkqglnth lgrahwehvs
      121 tmwkldnnsm tlkvapkltr shiypngfdk mclsfyslar spkdgnvspd vlesllsvee
      181 vlaekeslae dsvkplpida mevaadhrsy aerhsdarli yyisgyvark rvltth
//
LOCUS       XP_077506319             131 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115715 [Amblyomma americanum].
ACCESSION   XP_077506319
VERSION     XP_077506319.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650193.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..131
                     /product="uncharacterized protein LOC144115715"
                     /calculated_mol_wt=13515
     CDS             1..131
                     /gene="LOC144115715"
                     /coded_by="XM_077650193.1:141..536"
                     /db_xref="GeneID:144115715"
ORIGIN      
        1 marklfvlaa lvavaalaya edkpaaedkk ddvegrigfg ggfnqaaggn qygfnrgasg
       61 fnqgsggfdr gnrynnvqgf rnrdgyrtnq gfdqsssnry gsggggfntg fnrgaggayn
      121 qygqqgggfl g
//
LOCUS       XP_077506320             595 aa            linear   INV 25-NOV-2025
DEFINITION  SH2 domain-containing protein 4A-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506320
VERSION     XP_077506320.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650194.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..595
                     /product="SH2 domain-containing protein 4A-like isoform
                     X1"
                     /calculated_mol_wt=68302
     Region          286..>363
                     /region_name="tolA"
                     /note="cell envelope integrity inner membrane protein
                     TolA; Provisional; PRK09510"
                     /db_xref="CDD:236545"
     Region          476..578
                     /region_name="SH2"
                     /note="Src homology 2 (SH2) domain; cl15255"
                     /db_xref="CDD:472789"
     Site            order(490,511,531,533)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198173"
     Site            order(532,560)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198173"
     CDS             1..595
                     /gene="LOC144115717"
                     /coded_by="XM_077650194.1:742..2529"
                     /db_xref="GeneID:144115717"
ORIGIN      
        1 mivvthfgkp ltrkkgvkml qqilrdmyid pqllaeldde qkhilfckmr eeqvrrweqr
       61 ekelafnnkk ngttsnsskp gskrvdfllg sdgkpwtwvm gehpedssie eileretkek
      121 ariqaaqeal qlrkleeqqq kqqqqerlqq qkqqqlqeqk qqqqllqqqq lqqqqlqqwq
      181 qqqqqqqeqq qqeqqlqqqe qqlqqqqeqq lqqqeqlqqq qkeivvvpat pspppeiptd
      241 gieevvrlrr kkplegcfsl erevaallqd svatsrvatv drntwlsqkq qeirnreesl
      301 kqlmeqrsqe iyqeikaqqr ksieaaeres mvqeelwieq ekkakevekq ireiariare
      361 ehkralhltn gnmgtpvtgr davgmvspns kppvppkvvp pplpstpppn lvinggsngs
      421 vkngmsvttt tattvttgmp shatsrskda elfsradivq wflreeypqg avtdhngnnp
      481 aiwfhgfigr aeseqllqkq ggaaagaflv rvherikgyv isywagdrck hylidaslgh
      541 cqffgsnqlv fprlrdliwh httnpitalg qeqlltpcpr snsstlfsll ptlgq
//
LOCUS       XP_077506321             577 aa            linear   INV 25-NOV-2025
DEFINITION  SH2 domain-containing protein 4A-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506321
VERSION     XP_077506321.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650195.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..577
                     /product="SH2 domain-containing protein 4A-like isoform
                     X2"
                     /calculated_mol_wt=66281
     Region          268..>345
                     /region_name="tolA"
                     /note="cell envelope integrity inner membrane protein
                     TolA; Provisional; PRK09510"
                     /db_xref="CDD:236545"
     Region          458..560
                     /region_name="SH2"
                     /note="Src homology 2 (SH2) domain; cl15255"
                     /db_xref="CDD:472789"
     Site            order(472,493,513,515)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198173"
     Site            order(514,542)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198173"
     CDS             1..577
                     /gene="LOC144115717"
                     /coded_by="XM_077650195.1:339..2072"
                     /db_xref="GeneID:144115717"
ORIGIN      
        1 mlqqilrdmy idpqllaeld deqkhilfck mreeqvrrwe qrekelafnn kkngttsnss
       61 kpgskrvdfl lgsdgkpwtw vmgehpedss ieeileretk ekariqaaqe alqlrkleeq
      121 qqkqqqqerl qqqkqqqlqe qkqqqqllqq qqlqqqqlqq wqqqqqqqqe qqqqeqqlqq
      181 qeqqlqqqqe qqlqqqeqlq qqqkeivvvp atpspppeip tdgieevvrl rrkkplegcf
      241 slerevaall qdsvatsrva tvdrntwlsq kqqeirnree slkqlmeqrs qeiyqeikaq
      301 qrksieaaer esmvqeelwi eqekkakeve kqireiaria reehkralhl tngnmgtpvt
      361 grdavgmvsp nskppvppkv vppplpstpp pnlvinggsn gsvkngmsvt tttattvttg
      421 mpshatsrsk daelfsradi vqwflreeyp qgavtdhngn npaiwfhgfi graeseqllq
      481 kqggaaagaf lvrvherikg yvisywagdr ckhylidasl ghcqffgsnq lvfprlrdli
      541 whhttnpita lgqeqlltpc prsnsstlfs llptlgq
//
LOCUS       XP_077506322             577 aa            linear   INV 25-NOV-2025
DEFINITION  SH2 domain-containing protein 4A-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506322
VERSION     XP_077506322.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650196.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..577
                     /product="SH2 domain-containing protein 4A-like isoform
                     X2"
                     /calculated_mol_wt=66281
     Region          268..>345
                     /region_name="tolA"
                     /note="cell envelope integrity inner membrane protein
                     TolA; Provisional; PRK09510"
                     /db_xref="CDD:236545"
     Region          458..560
                     /region_name="SH2"
                     /note="Src homology 2 (SH2) domain; cl15255"
                     /db_xref="CDD:472789"
     Site            order(472,493,513,515)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198173"
     Site            order(514,542)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198173"
     CDS             1..577
                     /gene="LOC144115717"
                     /coded_by="XM_077650196.1:633..2366"
                     /db_xref="GeneID:144115717"
ORIGIN      
        1 mlqqilrdmy idpqllaeld deqkhilfck mreeqvrrwe qrekelafnn kkngttsnss
       61 kpgskrvdfl lgsdgkpwtw vmgehpedss ieeileretk ekariqaaqe alqlrkleeq
      121 qqkqqqqerl qqqkqqqlqe qkqqqqllqq qqlqqqqlqq wqqqqqqqqe qqqqeqqlqq
      181 qeqqlqqqqe qqlqqqeqlq qqqkeivvvp atpspppeip tdgieevvrl rrkkplegcf
      241 slerevaall qdsvatsrva tvdrntwlsq kqqeirnree slkqlmeqrs qeiyqeikaq
      301 qrksieaaer esmvqeelwi eqekkakeve kqireiaria reehkralhl tngnmgtpvt
      361 grdavgmvsp nskppvppkv vppplpstpp pnlvinggsn gsvkngmsvt tttattvttg
      421 mpshatsrsk daelfsradi vqwflreeyp qgavtdhngn npaiwfhgfi graeseqllq
      481 kqggaaagaf lvrvherikg yvisywagdr ckhylidasl ghcqffgsnq lvfprlrdli
      541 whhttnpita lgqeqlltpc prsnsstlfs llptlgq
//
LOCUS       XP_077506323             577 aa            linear   INV 25-NOV-2025
DEFINITION  SH2 domain-containing protein 4A-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506323
VERSION     XP_077506323.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650197.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..577
                     /product="SH2 domain-containing protein 4A-like isoform
                     X2"
                     /calculated_mol_wt=66281
     Region          268..>345
                     /region_name="tolA"
                     /note="cell envelope integrity inner membrane protein
                     TolA; Provisional; PRK09510"
                     /db_xref="CDD:236545"
     Region          458..560
                     /region_name="SH2"
                     /note="Src homology 2 (SH2) domain; cl15255"
                     /db_xref="CDD:472789"
     Site            order(472,493,513,515)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198173"
     Site            order(514,542)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198173"
     CDS             1..577
                     /gene="LOC144115717"
                     /coded_by="XM_077650197.1:333..2066"
                     /db_xref="GeneID:144115717"
ORIGIN      
        1 mlqqilrdmy idpqllaeld deqkhilfck mreeqvrrwe qrekelafnn kkngttsnss
       61 kpgskrvdfl lgsdgkpwtw vmgehpedss ieeileretk ekariqaaqe alqlrkleeq
      121 qqkqqqqerl qqqkqqqlqe qkqqqqllqq qqlqqqqlqq wqqqqqqqqe qqqqeqqlqq
      181 qeqqlqqqqe qqlqqqeqlq qqqkeivvvp atpspppeip tdgieevvrl rrkkplegcf
      241 slerevaall qdsvatsrva tvdrntwlsq kqqeirnree slkqlmeqrs qeiyqeikaq
      301 qrksieaaer esmvqeelwi eqekkakeve kqireiaria reehkralhl tngnmgtpvt
      361 grdavgmvsp nskppvppkv vppplpstpp pnlvinggsn gsvkngmsvt tttattvttg
      421 mpshatsrsk daelfsradi vqwflreeyp qgavtdhngn npaiwfhgfi graeseqllq
      481 kqggaaagaf lvrvherikg yvisywagdr ckhylidasl ghcqffgsnq lvfprlrdli
      541 whhttnpita lgqeqlltpc prsnsstlfs llptlgq
//
LOCUS       XP_077506324             372 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115718 [Amblyomma americanum].
ACCESSION   XP_077506324
VERSION     XP_077506324.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650198.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..372
                     /product="uncharacterized protein LOC144115718"
                     /calculated_mol_wt=40639
     Region          12..46
                     /region_name="F-box_SF"
                     /note="F-box domain superfamily; cl45894"
                     /db_xref="CDD:459239"
     Site            order(12,15..16,19..20,24,26..27,31..33,35)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438852"
     CDS             1..372
                     /gene="LOC144115718"
                     /coded_by="XM_077650198.1:350..1468"
                     /db_xref="GeneID:144115718"
ORIGIN      
        1 mdvssvvsqn fvvesvlnhl dtedlftcae vsrlwqsval sllrrklisv svccardpee
       61 lhvmppepsm hscvdifctk farfrnigrm agmhpslvfv schadlnedk rvveclrell
      121 ppdcvvvyfk lelvrgpdsd seevshegif geflfrpeal prvlssleds dgsesqqqpp
      181 daptatrpss prlqgrpddd etpatsaaaa petaagelvr aeaeplvwpr lpsvgsngga
      241 vfhnvlpgvl mfqalhvtea svegnatttr fmeeatrkmi qvtsaamyst mpstslqpfm
      301 rnlsdgfgpt tntiavafpr nqdearaeld aferafpnvp alanqatefn vdgqyaplgr
      361 lklilllikl ld
//
LOCUS       XP_077506325             340 aa            linear   INV 25-NOV-2025
DEFINITION  growth hormone-inducible transmembrane protein-like [Amblyomma
            americanum].
ACCESSION   XP_077506325
VERSION     XP_077506325.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650199.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..340
                     /product="growth hormone-inducible transmembrane
                     protein-like"
                     /calculated_mol_wt=35650
     Region          76..339
                     /region_name="GHITM"
                     /note="Growth-hormone inducible transmembrane protein;
                     cd10431"
                     /db_xref="CDD:198413"
     CDS             1..340
                     /gene="LOC144115722"
                     /coded_by="XM_077650199.1:257..1279"
                     /db_xref="GeneID:144115722"
ORIGIN      
        1 mlvmwkccls paaarasaka fasgptssir wptltqktvn mstrtrsarf qaatakrrtl
       61 reifmapage saftvgkgvl agasafglga lcyyglgmss eagayeksmv wpeyvrqrvr
      121 dtytyfggsi vitaasalav srhpamlnlv mrnswlaiga sfaamigtgm varslpyreg
      181 lgakqaawal hcgvmgalva plcllggpil traacytagv vsglstvaac apsekflnmg
      241 gplaiglgfv fasslgsmfl ppttalgagl yslsmygglv lfggfllydt qrivkmaety
      301 plyaaqpydp vnasisiyld tlnifiriam llagggsrrk
//
LOCUS       XP_077506326             340 aa            linear   INV 25-NOV-2025
DEFINITION  growth hormone-inducible transmembrane protein-like [Amblyomma
            americanum].
ACCESSION   XP_077506326
VERSION     XP_077506326.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650200.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..340
                     /product="growth hormone-inducible transmembrane
                     protein-like"
                     /calculated_mol_wt=35650
     Region          76..339
                     /region_name="GHITM"
                     /note="Growth-hormone inducible transmembrane protein;
                     cd10431"
                     /db_xref="CDD:198413"
     CDS             1..340
                     /gene="LOC144115722"
                     /coded_by="XM_077650200.1:223..1245"
                     /db_xref="GeneID:144115722"
ORIGIN      
        1 mlvmwkccls paaarasaka fasgptssir wptltqktvn mstrtrsarf qaatakrrtl
       61 reifmapage saftvgkgvl agasafglga lcyyglgmss eagayeksmv wpeyvrqrvr
      121 dtytyfggsi vitaasalav srhpamlnlv mrnswlaiga sfaamigtgm varslpyreg
      181 lgakqaawal hcgvmgalva plcllggpil traacytagv vsglstvaac apsekflnmg
      241 gplaiglgfv fasslgsmfl ppttalgagl yslsmygglv lfggfllydt qrivkmaety
      301 plyaaqpydp vnasisiyld tlnifiriam llagggsrrk
//
LOCUS       XP_077506327             340 aa            linear   INV 25-NOV-2025
DEFINITION  growth hormone-inducible transmembrane protein-like [Amblyomma
            americanum].
ACCESSION   XP_077506327
VERSION     XP_077506327.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650201.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..340
                     /product="growth hormone-inducible transmembrane
                     protein-like"
                     /calculated_mol_wt=35650
     Region          76..339
                     /region_name="GHITM"
                     /note="Growth-hormone inducible transmembrane protein;
                     cd10431"
                     /db_xref="CDD:198413"
     CDS             1..340
                     /gene="LOC144115722"
                     /coded_by="XM_077650201.1:174..1196"
                     /db_xref="GeneID:144115722"
ORIGIN      
        1 mlvmwkccls paaarasaka fasgptssir wptltqktvn mstrtrsarf qaatakrrtl
       61 reifmapage saftvgkgvl agasafglga lcyyglgmss eagayeksmv wpeyvrqrvr
      121 dtytyfggsi vitaasalav srhpamlnlv mrnswlaiga sfaamigtgm varslpyreg
      181 lgakqaawal hcgvmgalva plcllggpil traacytagv vsglstvaac apsekflnmg
      241 gplaiglgfv fasslgsmfl ppttalgagl yslsmygglv lfggfllydt qrivkmaety
      301 plyaaqpydp vnasisiyld tlnifiriam llagggsrrk
//
LOCUS       XP_077506329             510 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115723 [Amblyomma americanum].
ACCESSION   XP_077506329
VERSION     XP_077506329.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650203.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..510
                     /product="uncharacterized protein LOC144115723"
                     /calculated_mol_wt=54680
     Region          281..506
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cl21584"
                     /db_xref="CDD:473915"
     Site            order(313,356,459)
                     /site_type="active"
                     /db_xref="CDD:238113"
     Site            order(453,478,480)
                     /site_type="other"
                     /note="substrate binding sites [chemical binding]"
                     /db_xref="CDD:238113"
     CDS             1..510
                     /gene="LOC144115723"
                     /coded_by="XM_077650203.1:217..1749"
                     /db_xref="GeneID:144115723"
ORIGIN      
        1 mvtaptstka tavrftpgpv tllllllaqt grvasypgkv vfeeeevnkc slsephcvtv
       61 aqcpqaqrni rrgthpticd wkgatslvcc aqptgvppvt pdqphpptev pppippvepp
      121 vgppveppvq ppsvvppisp pdsqpppptd qgtgtnvpev pgtggtssvd nagssgakpd
      181 gasegrdpaa gdedcecdda lpvrtcglrp eatesrqrpa rllldepvys ldarrvrrpg
      241 vsrrrrplna vsllprqqli gqpqqpqqqq qqqqqspsds swpwmasvvd qvnparrcag
      301 vlldqqhilt aaqcfgpdng rnsavmlntp glpvtlrrvv saeahpmfrv pdgyhdlalv
      361 rfepalaage lapiclptvl narrvlngsv ldavswtnrq rdfhpgsmge kryrvissss
      421 cnntyagvdl spypqginhe dfictqlttn igeecvgipg splmiqegkn waviglysfg
      481 vscsgtaqyp tvftrvspyi twiyeklgvr
//
LOCUS       XP_077506330             142 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115724 [Amblyomma americanum].
ACCESSION   XP_077506330
VERSION     XP_077506330.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650204.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..142
                     /product="uncharacterized protein LOC144115724"
                     /calculated_mol_wt=14854
     CDS             1..142
                     /gene="LOC144115724"
                     /coded_by="XM_077650204.1:122..550"
                     /db_xref="GeneID:144115724"
ORIGIN      
        1 mlskmlkrtg elqehlkkrk tcqcseqqqv cnsdipatre pelgseslga rdvpgeglae
       61 laaaaptave aaassspspa qqglpvqaqi yvptssviaq erqgaprggd tqnggrenee
      121 rpsrctwksi paskrgrsac aa
//
LOCUS       XP_077506331             695 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115725 [Amblyomma americanum].
ACCESSION   XP_077506331
VERSION     XP_077506331.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650205.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..695
                     /product="uncharacterized protein LOC144115725"
                     /calculated_mol_wt=77801
     Region          237..380
                     /region_name="Transposase_21"
                     /note="Transposase family tnp2; cl29371"
                     /db_xref="CDD:475188"
     CDS             1..695
                     /gene="LOC144115725"
                     /coded_by="XM_077650205.1:936..3023"
                     /db_xref="GeneID:144115725"
ORIGIN      
        1 msmetevmsk wrryriieke yrellgelgi rrppsthlks sgskrtggea aldvvsappl
       61 plpsgaskrp nseayasgac asgsgratss lpadpsmpsv qselneqayv sescaeslss
      121 ehtatscdss detsstnslt aenvemgqpr eespelcyrh lstaqelasi askhnlthac
      181 indlldfcrr rgivelpkda rtimqterka nlehgdsfvh fglaegiqha vgqgpapenv
      241 elhanidglp lhrssqtalw pilcrvinik ksdpfmvsay cgagkppclq eylrpfidev
      301 rnlscnglmv kgvhvnvclk avicdapars fvkaitghtg yhacercsqk ghylenrmtf
      361 pdlhapqrtd ysfrsqedrr hhtgvspfts lnvdmvkcfp teymhlvclg vmrrllrnwi
      421 ckghaarlsr adrsalndkl reaatafpky fqrkprgtee ldrwkatefr tfllylgpvl
      481 lksilpdely khflylhvsv rvlaspqhhr dlnqfahdll ryfvqefgrl ygqkqlvynv
      541 htlahlaeqc lehgtvdefs afpfesylgk vkkrlrttnk plaqlsrrms evractplst
      601 gqvggdvkvg dcflvdncav vivdllggna kagilpngre ffrvpidssr mdvrrysalg
      661 qetkiwpisy iknkvrcikl qykrghvvfp llhlq
//
LOCUS       XP_077506332             187 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115726 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506332
VERSION     XP_077506332.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650206.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="uncharacterized protein LOC144115726 isoform X1"
                     /calculated_mol_wt=21208
     CDS             1..187
                     /gene="LOC144115726"
                     /coded_by="XM_077650206.1:219..782"
                     /db_xref="GeneID:144115726"
ORIGIN      
        1 mrerrkikar sgaspdelyq pkwahykslm fldtscssqp sfstlhtqee eeeterqceh
       61 wnnesadqem dfpcssqnca epslpaqqig egtsrpgkkr rrnddeteqr kvllttavet
      121 lqrsqqrlaa ndecdafgsm mahavrqipp gpdrqlamln ahqaivkinl srhsvpveml
      181 nvlnmqe
//
LOCUS       XP_077506333             187 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115726 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506333
VERSION     XP_077506333.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650207.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="uncharacterized protein LOC144115726 isoform X1"
                     /calculated_mol_wt=21208
     CDS             1..187
                     /gene="LOC144115726"
                     /coded_by="XM_077650207.1:330..893"
                     /db_xref="GeneID:144115726"
ORIGIN      
        1 mrerrkikar sgaspdelyq pkwahykslm fldtscssqp sfstlhtqee eeeterqceh
       61 wnnesadqem dfpcssqnca epslpaqqig egtsrpgkkr rrnddeteqr kvllttavet
      121 lqrsqqrlaa ndecdafgsm mahavrqipp gpdrqlamln ahqaivkinl srhsvpveml
      181 nvlnmqe
//
LOCUS       XP_077506334             185 aa            linear   INV 25-NOV-2025
DEFINITION  regulator of chromosome condensation-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506334
VERSION     XP_077506334.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650208.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..185
                     /product="regulator of chromosome condensation-like
                     isoform X2"
                     /calculated_mol_wt=19368
     CDS             1..185
                     /gene="LOC144115726"
                     /coded_by="XM_077650208.1:1316..1873"
                     /db_xref="GeneID:144115726"
ORIGIN      
        1 mvilmtegnl ltrgcaelgq lgwvapmfas cggrrssayl lepgtvrvqc peiasgndiv
       61 vmlradrnlv tvgcaeqgql grvapmftsr ggrrsftcll epgtvtvqrp tatsfagvnl
      121 disftgdilg ralstgsssv atgcvscalr yhelpsrlds rghtqpgcgt tvtarlsrkm
      181 rdgts
//
LOCUS       XP_077506335             185 aa            linear   INV 25-NOV-2025
DEFINITION  regulator of chromosome condensation-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506335
VERSION     XP_077506335.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650209.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..185
                     /product="regulator of chromosome condensation-like
                     isoform X2"
                     /calculated_mol_wt=19368
     CDS             1..185
                     /gene="LOC144115726"
                     /coded_by="XM_077650209.1:1261..1818"
                     /db_xref="GeneID:144115726"
ORIGIN      
        1 mvilmtegnl ltrgcaelgq lgwvapmfas cggrrssayl lepgtvrvqc peiasgndiv
       61 vmlradrnlv tvgcaeqgql grvapmftsr ggrrsftcll epgtvtvqrp tatsfagvnl
      121 disftgdilg ralstgsssv atgcvscalr yhelpsrlds rghtqpgcgt tvtarlsrkm
      181 rdgts
//
LOCUS       XP_077506336             185 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115726 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077506336
VERSION     XP_077506336.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650210.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..185
                     /product="uncharacterized protein LOC144115726 isoform X3"
                     /calculated_mol_wt=19378
     CDS             1..185
                     /gene="LOC144115726"
                     /coded_by="XM_077650210.1:162..719"
                     /db_xref="GeneID:144115726"
ORIGIN      
        1 mvilttegnl vtrgcaelgq lgwvapmfas cggrrssayl lepgtvrvqc peiasgndiv
       61 vmlradrnlv tvgcaeqgql grvapmftsr ggrrsftcll epgtvtvqrp tatslagvnl
      121 disftgdilg rslstgsssv atgcvscalr yhelpsrlds rghtqpecgt tvtarlsrkm
      181 rdgts
//
LOCUS       XP_077506337             185 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115726 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077506337
VERSION     XP_077506337.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650211.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..185
                     /product="uncharacterized protein LOC144115726 isoform X3"
                     /calculated_mol_wt=19378
     CDS             1..185
                     /gene="LOC144115726"
                     /coded_by="XM_077650211.1:162..719"
                     /db_xref="GeneID:144115726"
ORIGIN      
        1 mvilttegnl vtrgcaelgq lgwvapmfas cggrrssayl lepgtvrvqc peiasgndiv
       61 vmlradrnlv tvgcaeqgql grvapmftsr ggrrsftcll epgtvtvqrp tatslagvnl
      121 disftgdilg rslstgsssv atgcvscalr yhelpsrlds rghtqpecgt tvtarlsrkm
      181 rdgts
//
LOCUS       XP_077506338             132 aa            linear   INV 25-NOV-2025
DEFINITION  regulator of chromosome condensation-like isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077506338
VERSION     XP_077506338.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650212.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..132
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..132
                     /product="regulator of chromosome condensation-like
                     isoform X4"
                     /calculated_mol_wt=14068
     CDS             1..132
                     /gene="LOC144115726"
                     /coded_by="XM_077650212.1:162..560"
                     /db_xref="GeneID:144115726"
ORIGIN      
        1 mvilttegnl vtrgcaelgq lgwvapmfas cggrrssayl lepgtvrvqc peiasgndiv
       61 vmlradrnlv tvgcaeqgql grvapmftsr ggrrsftcll epgtvtvqrp talgqveqpa
      121 sswswrhsgk hr
//
LOCUS       XP_077506339             159 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115728 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506339
VERSION     XP_077506339.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650213.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..159
                     /product="uncharacterized protein LOC144115728 isoform X1"
                     /calculated_mol_wt=16918
     CDS             1..159
                     /gene="LOC144115728"
                     /coded_by="XM_077650213.1:1359..1838"
                     /db_xref="GeneID:144115728"
ORIGIN      
        1 mfasrgerss stcllepgtv rlqcprdrlw qrhcgdaesa pklgdhgmcr agtagtsgsh
       61 vhitwwtqvi hlpagawhvr vqrptatsfa gvnldisftg dilgralstg sssvatgcvs
      121 calryqelps rlesrghtqp gcgttvtarl srkmrdgts
//
LOCUS       XP_077506340             159 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115728 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506340
VERSION     XP_077506340.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650214.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..159
                     /product="uncharacterized protein LOC144115728 isoform X1"
                     /calculated_mol_wt=16918
     CDS             1..159
                     /gene="LOC144115728"
                     /coded_by="XM_077650214.1:1304..1783"
                     /db_xref="GeneID:144115728"
ORIGIN      
        1 mfasrgerss stcllepgtv rlqcprdrlw qrhcgdaesa pklgdhgmcr agtagtsgsh
       61 vhitwwtqvi hlpagawhvr vqrptatsfa gvnldisftg dilgralstg sssvatgcvs
      121 calryqelps rlesrghtqp gcgttvtarl srkmrdgts
//
LOCUS       XP_077506342             159 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115728 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506342
VERSION     XP_077506342.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650216.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..159
                     /product="uncharacterized protein LOC144115728 isoform X1"
                     /calculated_mol_wt=16918
     CDS             1..159
                     /gene="LOC144115728"
                     /coded_by="XM_077650216.1:1359..1838"
                     /db_xref="GeneID:144115728"
ORIGIN      
        1 mfasrgerss stcllepgtv rlqcprdrlw qrhcgdaesa pklgdhgmcr agtagtsgsh
       61 vhitwwtqvi hlpagawhvr vqrptatsfa gvnldisftg dilgralstg sssvatgcvs
      121 calryqelps rlesrghtqp gcgttvtarl srkmrdgts
//
LOCUS       XP_077506343             136 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115728 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506343
VERSION     XP_077506343.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650217.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..136
                     /product="uncharacterized protein LOC144115728 isoform X2"
                     /calculated_mol_wt=14339
     CDS             1..136
                     /gene="LOC144115728"
                     /coded_by="XM_077650217.1:1250..1660"
                     /db_xref="GeneID:144115728"
ORIGIN      
        1 mpgdrlwqrh cgdaesapkl gdhgmcragt agtsgshvhi twwtqvihlp agawhvrvqr
       61 ptatsfagvn ldisftgdil gralstgsss vatgcvscal ryqelpsrle srghtqpgcg
      121 ttvtarlsrk mrdgts
//
LOCUS       XP_077506344             288 aa            linear   INV 25-NOV-2025
DEFINITION  band 7 protein AGAP004871-like [Amblyomma americanum].
ACCESSION   XP_077506344
VERSION     XP_077506344.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650218.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..288
                     /product="band 7 protein AGAP004871-like"
                     /calculated_mol_wt=31507
     Region          74..275
                     /region_name="SPFH_stomatin"
                     /note="Stomatin, a subgroup of the stomatin-like proteins
                     (slipins) family; belonging to the SPFH (stomatin,
                     prohibitin, flotillin, and HflK/C) superfamily; cd03403"
                     /db_xref="CDD:259801"
     CDS             1..288
                     /gene="LOC144115729"
                     /coded_by="XM_077650218.1:567..1433"
                     /db_xref="GeneID:144115729"
ORIGIN      
        1 mekdagvapa tayrtgynvm ssrssdgtnv csiiltfisl lvisftfpfs lffcikivqe
       61 yeravifrlg rlvqggakgp glffiipcid tyckvdlrtv sfdvppqeil skdsvtvavd
      121 avvyyrisna tiaitnvedy ghstrllaat tlrnvlgtkn lseilseres ishvmqasld
      181 eatdswgvkv erveikdvrl pvqlqramaa eaeaareara kviaaegeqr asrslkeaae
      241 viaespsalq lrylqtltsi aaeknstivf plplelmrgl lardhddd
//
LOCUS       XP_077506345             949 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115730 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506345
VERSION     XP_077506345.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650219.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..949
                     /product="uncharacterized protein LOC144115730 isoform X1"
                     /calculated_mol_wt=107360
     Region          6..82
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     Region          287..>429
                     /region_name="Tnp_P_element"
                     /note="Transposase protein; pfam12017"
                     /db_xref="CDD:288840"
     CDS             1..949
                     /gene="LOC144115730"
                     /coded_by="XM_077650219.1:80..2929"
                     /db_xref="GeneID:144115730"
ORIGIN      
        1 mshmyccakw cstsgksgeh lfrfpsdhrf siwlkyanrl elletprekv yktyrlcskh
       61 ftrdcftsda ctrltheavp svkvheprlr alvhpdfyeg gaaqgiesas plqstaapve
      121 vmlghdylpl pstlglgtia gadsavhdtd dphssrapql tdvqttpppp adeegaaqst
      181 pirviaspsw dspdyaeagp sglsgakesv rqpvmtpasg tpksrrrkga vvytprtkkr
      241 feklhsrsdr yrkalsrfrk rhdtrrvtqq ealnklephl pqdlfelvka qirlcsfpks
      301 kkhwskqmkq falnlffhsp kayrflreml hlphvrtlrr wladipmtpg iipgamdaie
      361 sitkdwplqd kaccllfdei slkknlmydq skdivigftd dgntrtsrva nialvmavsg
      421 isrswmqpva faashnatsv lamrkllldl iqqlqtkgll vkavicdqgs nnvslsrmli
      481 hsihepyfvv nnrkiyfmfd tphlikctrn nlrrhkltig sevvdwthie tlykktrhid
      541 rasktpvehl ksrlakklke qhiyrmpfad mrvkyatqvf sesvsvallt liaiqelpvd
      601 akftaefter mdklfdclns salkkqddkl ryamtegseh hvflescidw iaqwhfggpl
      661 dkqphtikgw qitikallll wtdlkadfdy thlftrrlqq dplenffaiv rqkhgcnetp
      721 nvyqfvaavk hiwigqlfkl sqgncevtth afltnlesvs avlspsstps tshsrqqgsg
      781 ssdlqassqd apdivyenvv yhvagtlvks fldqrttect cegtllaadg gslhgrhqff
      841 allkengvpe hlfqsiaats eaclnyikkl dsafrkeisc cahlsnvsrn lvermpnqqa
      901 vfcslgckik flklfcrtrl hwhlsflnrs mtsktkqsap arkrrklag
//
LOCUS       XP_077506346             183 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115730 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506346
VERSION     XP_077506346.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650220.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..183
                     /product="uncharacterized protein LOC144115730 isoform X2"
                     /calculated_mol_wt=20120
     Region          6..82
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..183
                     /gene="LOC144115730"
                     /coded_by="XM_077650220.1:80..631"
                     /db_xref="GeneID:144115730"
ORIGIN      
        1 mshmyccakw cstsgksgeh lfrfpsdhrf siwlkyanrl elletprekv yktyrlcskh
       61 ftrdcftsda ctrltheavp svkvheprlr alvhpdfyeg gaaqgiesas plqstaapve
      121 vmlghdylpl pstlglgtia gadsavhdtd dphssrapql tdvqttpppp adeegrcnal
      181 ivq
//
LOCUS       XP_077506347             937 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115731 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506347
VERSION     XP_077506347.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650221.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..937
                     /product="uncharacterized protein LOC144115731 isoform X1"
                     /calculated_mol_wt=102981
     CDS             1..937
                     /gene="LOC144115731"
                     /coded_by="XM_077650221.1:177..2990"
                     /db_xref="GeneID:144115731"
ORIGIN      
        1 meledkqcsd avhgsvdlhd lgdthdpisc rdlvncshpk isgdpvnphg slrrplssta
       61 esphgstaeh fpgpavdqha ngstqhgsav ehqldavehh cdagkhpcdv gehpcdsvhr
      121 phdsdehsnd yveradggke vkpefkanld algqdspvah scgadttvmp qkakrqeaee
      181 dlprtespth keggsrreas pqkevkkrav tisipsylgw psaaakeadl eakksdhtdc
      241 vlvhaysrea dvlcfwpkds sfvkqvrrrt aegglgplep rfvdgvikvp pgsyrcrval
      301 prktfeldpt rvkknsetkv lknndakpqr htckklssak keesgedyye ldsdflrvps
      361 cglsynlpqg keahrvplad vnssstaasq rkpcntgkkr gpiepsddas sssprfeqha
      421 nrtqptfhat dtearqgrar ylntrfrnis denrppqdrp lswdeflpre pwttcspipi
      481 erksvwaitr tasperprfe gpparkgkmk pgkkslasll rrtmisepnp hfemprkgrs
      541 katcdvrqmd geprtttass egvklfsgqk egikdlhspn tsprkasslm pkirgmvese
      601 dqqhlseklh ealfsgegfi pvnegikesa kirrgaisil spraearrrl ffpdpegnie
      661 lgssqksgeq qiprsriarh apissvtpek vlppaltksa kvgqhatnla garagkrkpf
      721 skllaawgwq snesigevep rqkeekptsw psfngkpeka tgalsrnvas eqpepaeqkg
      781 qqstevvngg sqaeyqnast qtshecmfgr kvktgseadr pkpmtpilke kdvefidwmp
      841 tlnekntpft fgtvavtnsa svgmlklnpd qekqmaadna hnlflyalds gvmvtlvega
      901 wtllakrstf iiqkgspftl knvgvslakv lyvladp
//
LOCUS       XP_077506348             903 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115731 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506348
VERSION     XP_077506348.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650222.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..903
                     /product="uncharacterized protein LOC144115731 isoform X2"
                     /calculated_mol_wt=99583
     CDS             1..903
                     /gene="LOC144115731"
                     /coded_by="XM_077650222.1:178..2889"
                     /db_xref="GeneID:144115731"
ORIGIN      
        1 meledkqcsd avhgsvdlhd lgdthdpisc rdlvncshpk isgdpvnphg slrrplssta
       61 esphgstaeh fpgpavdqha ngstqhgsav ehqldavehh cdagkhpcdv gehpcdsvhr
      121 phdsdehsnd yveradggke vkpefkanld algqdspvah scgadttvmp qkakrqeaee
      181 dlprtespth keggsrreas pqkevkkrav tisipsylgw psaaakeadl eakksdhtdc
      241 vlvhaysrea dvlcfwpkds sfvkqvrrrt aegglgplep rfvdgvikvp pgsyrcrval
      301 prktfeldpt rvkknsetkv lknndakpqr htckklssak keesgedyye ldsdflrvps
      361 cglsynlpqg keahrvplad vnssstaasq rkpcntgkkr gpiepsddas sssprfeqha
      421 nrtqptfhat dtearqgrar ylntrfrnis denrppqdrp lswdeflpre pwttcspipi
      481 erksvwaitr tasperprfe gpparkgkmk pgkkslasll rrtmisepnp hfemprkgrs
      541 katcdvrqmd geprtttass egvklfsgqk egikdlhspn tsprkasslm pkirgmvese
      601 dqqhlseklh ealfsgegfi pvnegikesa kirrgaisil spraearrrl ffpdpegnie
      661 lgssqksgeq qiprsriarh apissvtpek vlppaltksa kvgqhatnla garagkrkpf
      721 skllaawgwq snesigevep rqkeekptsw psfngkpeka tgalsrnvas eqpepaeqkg
      781 qqstevvngg sqaeyqnast qtshecmfgr kvktgseadr pkpmilicsg qwsdghtrrr
      841 cldvvgqtih iyhpkrqpvh sqecgsflgq gpvcacrsln lgetlkcliq nlysllcffy
      901 sey
//
LOCUS       XP_077506349             160 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115731 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077506349
VERSION     XP_077506349.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650223.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..160
                     /product="uncharacterized protein LOC144115731 isoform X3"
                     /calculated_mol_wt=17142
     CDS             1..160
                     /gene="LOC144115731"
                     /coded_by="XM_077650223.1:178..660"
                     /db_xref="GeneID:144115731"
ORIGIN      
        1 meledkqcsd avhgsvdlhd lgdthdpisc rdlvncshpk isgdpvnphg slrrplssta
       61 esphgstaeh fpgpavdqha ngstqhgsav ehqldavehh cdagkhpcdv gehpcdsvhr
      121 phdsdehsnd yveradggke vkpefkanld algqdrrfpg
//
LOCUS       XP_077506350             155 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115731 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077506350
VERSION     XP_077506350.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650224.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..155
                     /product="uncharacterized protein LOC144115731 isoform X4"
                     /calculated_mol_wt=16797
     Region          79..151
                     /region_name="cupin_RmlC-like"
                     /note="RmlC-like cupin superfamily; cl40423"
                     /db_xref="CDD:477354"
     CDS             1..155
                     /gene="LOC144115731"
                     /coded_by="XM_077650224.1:110..577"
                     /db_xref="GeneID:144115731"
ORIGIN      
        1 mllgktggsq aeyqnastqt shecmfgrkv ktgseadrpk pmtpilkekd vefidwmptl
       61 nekntpftfg tvavtnsasv gmlklnpdqe kqmaadnahn lflyaldsgv mvtlvegawt
      121 llakrstfii qkgspftlkn vgvslakvly vladp
//
LOCUS       XP_077506351             164 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115734 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506351
VERSION     XP_077506351.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650225.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..164
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..164
                     /product="uncharacterized protein LOC144115734 isoform X1"
                     /calculated_mol_wt=17784
     CDS             1..164
                     /gene="LOC144115734"
                     /coded_by="XM_077650225.1:314..808"
                     /db_xref="GeneID:144115734"
ORIGIN      
        1 msplsrlgav lvcarsayia ehncfsiinr tmaatcswys gqvkskksgn mtagvselkv
       61 ekamydtmkg ipkvavlhkr tattiwseea vvqcsssgkc sgdqkasthw getadeatkl
      121 imktllktpv alrnrlkgqn ekeafenlrl ysaivddaes qqpp
//
LOCUS       XP_077506352             148 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115734 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506352
VERSION     XP_077506352.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650226.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..148
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..148
                     /product="uncharacterized protein LOC144115734 isoform X2"
                     /calculated_mol_wt=15845
     CDS             1..148
                     /gene="LOC144115734"
                     /coded_by="XM_077650226.1:311..757"
                     /db_xref="GeneID:144115734"
ORIGIN      
        1 msplsrlgav lvcarsayia ehncfsiinr tmaatcswys gqvkskksgn mtagvselkv
       61 ekamydtmkg ipkvavlhkr tattiwseea vvqcsssgkc sgvqnlnshh rrhqqqsggv
      121 pawgerlgwr lessdeeggp ltpvigal
//
LOCUS       XP_077506353              93 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115734 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077506353
VERSION     XP_077506353.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650227.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..93
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..93
                     /product="uncharacterized protein LOC144115734 isoform X3"
                     /calculated_mol_wt=9921
     CDS             1..93
                     /gene="LOC144115734"
                     /coded_by="XM_077650227.1:314..595"
                     /db_xref="GeneID:144115734"
ORIGIN      
        1 msplsrlgav lvcarsayia ehncfsiinr tmaatcswys gqvkskksgn mtagvselkv
       61 ekamydtmkg ipkvavlhkr tatticaesq qpp
//
LOCUS       XP_077506355             284 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115735 [Amblyomma americanum].
ACCESSION   XP_077506355
VERSION     XP_077506355.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650229.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..284
                     /product="uncharacterized protein LOC144115735"
                     /calculated_mol_wt=32049
     Region          <63..>141
                     /region_name="Cast"
                     /note="RIM-binding protein of the cytomatrix active zone;
                     pfam10174"
                     /db_xref="CDD:431111"
     CDS             1..284
                     /gene="LOC144115735"
                     /coded_by="XM_077650229.1:92..946"
                     /db_xref="GeneID:144115735"
ORIGIN      
        1 mqvrksyslf akkssnyfli qlpspqcctc ivvecfavvh sllmqsgdie tnpgpdtaai
       61 laelqkltsg qstlisevkd lssrlvatdk sitelskrit elethyqdla slktevesik
      121 tdaatsasqi snleyrvdda enrsrrnnll fyglpdtssk etsseseelv trlcsenldi
      181 tidpkeiera hrlgrhsser crpiivkftf hktkesilkn arklkgtdys igedfshsvr
      241 karkhllrfa ketsdkyalr fktlyigpkq yifddltetv kevp
//
LOCUS       XP_077506356             230 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115736 [Amblyomma americanum].
ACCESSION   XP_077506356
VERSION     XP_077506356.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650230.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..230
                     /product="uncharacterized protein LOC144115736"
                     /calculated_mol_wt=24799
     CDS             1..230
                     /gene="LOC144115736"
                     /coded_by="XM_077650230.1:1..693"
                     /db_xref="GeneID:144115736"
ORIGIN      
        1 mfypwpglip cvyrrhakai titrvgassa vvgcsrrava nisawssggp vtrgplvigr
       61 spsiprskad trysstrltw sthgwrtlfh nvrggaptgp pvpvgvlrrw qaachrhrpg
      121 prggaahaed frgqapntpa aanrrntsss pngqcdelgd gcsrhpsaas evlamamhep
      181 ypgrqwrsvt fddakrrvia tdqdaevdtl nmkctvgrlp tlrpsrtdcc
//
LOCUS       XP_077506357             262 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115740 [Amblyomma americanum].
ACCESSION   XP_077506357
VERSION     XP_077506357.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650231.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..262
                     /product="uncharacterized protein LOC144115740"
                     /calculated_mol_wt=30070
     CDS             1..262
                     /gene="LOC144115740"
                     /coded_by="XM_077650231.1:279..1067"
                     /db_xref="GeneID:144115740"
ORIGIN      
        1 mrpveifvle halkyqmgsa vkalrgrgnk qtivynlssp yiclmylvhi fngyltssyk
       61 tisswdpavl kvlvdvfqdm ysrekftsek vlvplilrar lleiivyaek alppgfvlek
      121 ldtyfptpte atwkefaqty slkgksmtwl ldtckksrqy vmnlvvkkkr lesdshdleg
      181 fvvtvkrlvd qiatdlskti prtglekild geklqldrdt eqiiqtvisk kqpimekhll
      241 qllelqesme hpvelnsitd nl
//
LOCUS       XP_077506358             112 aa            linear   INV 25-NOV-2025
DEFINITION  bolA-like protein 1 [Amblyomma americanum].
ACCESSION   XP_077506358
VERSION     XP_077506358.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650232.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..112
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..112
                     /product="bolA-like protein 1"
                     /calculated_mol_wt=12469
     Region          29..93
                     /region_name="BolA"
                     /note="BolA-like protein; cl00386"
                     /db_xref="CDD:469753"
     CDS             1..112
                     /gene="LOC144115741"
                     /coded_by="XM_077650232.1:286..624"
                     /db_xref="GeneID:144115741"
ORIGIN      
        1 mqrcfafmer rlltcgatyf vtraasggqt earilkklke altpqellve neshmhnvpk
       61 atrhgvrrpe ggtrkpghal aiqtstpdqr qskteksppc lggmkrerrs ke
//
LOCUS       XP_077506359              98 aa            linear   INV 25-NOV-2025
DEFINITION  dynactin subunit 4-like [Amblyomma americanum].
ACCESSION   XP_077506359
VERSION     XP_077506359.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650233.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..98
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..98
                     /product="dynactin subunit 4-like"
                     /calculated_mol_wt=11101
     CDS             1..98
                     /gene="LOC144115744"
                     /coded_by="XM_077650233.1:9..305"
                     /db_xref="GeneID:144115744"
ORIGIN      
        1 mceftlpard dtaefddsse nifvddpsvv tfrkanslgf yirvipseee avivafrlkh
       61 dftnmvvqlq adhrepqvvw mthtvvvnlg trsyrpps
//
LOCUS       XP_077506360             157 aa            linear   INV 25-NOV-2025
DEFINITION  dynactin subunit 4-like [Amblyomma americanum].
ACCESSION   XP_077506360
VERSION     XP_077506360.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650234.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..157
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..157
                     /product="dynactin subunit 4-like"
                     /calculated_mol_wt=17715
     CDS             1..157
                     /gene="LOC144115745"
                     /coded_by="XM_077650234.1:32..505"
                     /db_xref="GeneID:144115745"
ORIGIN      
        1 mlhshhipei kirsvtafdi geecyvqlsm ynptphvthv tllpleqaie gitakvllpm
       61 ceftlpardd taefddssen ifvddpsvvt frkanslgfy irvipseeea vivafrlkhd
      121 ftnmvvqlqa dhrepqvvwm thtvvvnlgt rsyrpps
//
LOCUS       XP_077506363             325 aa            linear   INV 25-NOV-2025
DEFINITION  mortality factor 4-like protein 1 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506363
VERSION     XP_077506363.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650237.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..325
                     /product="mortality factor 4-like protein 1 isoform X1"
                     /calculated_mol_wt=38051
     Region          11..67
                     /region_name="CBD_MSL3_like"
                     /note="chromo barrel domain of human male-specific lethal
                     complex subunit 3, and similar proteins; cd18983"
                     /db_xref="CDD:350846"
     Site            order(17..18,22..25,40,42..45,49,51..54,56..58,61..62)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:350846"
     Site            order(22,42,45,49)
                     /site_type="other"
                     /note="putative methylated histone tail binding site
                     [chemical binding]"
                     /db_xref="CDD:350846"
     Site            order(39,41..46,48..52)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:350846"
     Site            order(39,42,46,48,52)
                     /site_type="other"
                     /note="putative RNA binding site [nucleotide binding]"
                     /db_xref="CDD:350846"
     Region          150..313
                     /region_name="MRG"
                     /note="pfam05712"
                     /db_xref="CDD:461721"
     CDS             1..325
                     /gene="LOC144115746"
                     /coded_by="XM_077650237.1:522..1499"
                     /db_xref="GeneID:144115746"
ORIGIN      
        1 mdltnrfqed ekvlifdgpl iyeakclkvr irdkkaqyfv hyrgwnkswd ewipesrvlk
       61 yddinlqkkk eldeaynemk kkkpepdpee eidlddssvs seekprkrkg trvassvvsn
      121 rrsttraaes gggrqrrkrs rrdtqvepge tslsreefkv cipdelkpwl lddwylvtqq
      181 kmlvqvpcsv tvdqiladyv tektsqpnts shqaritevt ngfkkyfnvm lgsqllykfe
      241 rpqyadilee rrgtpmsqiy gsihllrlfv rldsmlktvp lekasvenll aevedflqyl
      301 arnshqlftf ddystatrey irral
//
LOCUS       XP_077506364             306 aa            linear   INV 25-NOV-2025
DEFINITION  mortality factor 4-like protein 1 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506364
VERSION     XP_077506364.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650238.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..306
                     /product="mortality factor 4-like protein 1 isoform X2"
                     /calculated_mol_wt=36093
     Region          11..67
                     /region_name="CBD_MSL3_like"
                     /note="chromo barrel domain of human male-specific lethal
                     complex subunit 3, and similar proteins; cd18983"
                     /db_xref="CDD:350846"
     Site            order(17..18,22..25,40,42..45,49,51..54,56..58,61..62)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:350846"
     Site            order(22,42,45,49)
                     /site_type="other"
                     /note="putative methylated histone tail binding site
                     [chemical binding]"
                     /db_xref="CDD:350846"
     Site            order(39,41..46,48..52)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:350846"
     Site            order(39,42,46,48,52)
                     /site_type="other"
                     /note="putative RNA binding site [nucleotide binding]"
                     /db_xref="CDD:350846"
     Region          131..294
                     /region_name="MRG"
                     /note="pfam05712"
                     /db_xref="CDD:461721"
     CDS             1..306
                     /gene="LOC144115746"
                     /coded_by="XM_077650238.1:522..1442"
                     /db_xref="GeneID:144115746"
ORIGIN      
        1 mdltnrfqed ekvlifdgpl iyeakclkvr irdkkaqyfv hyrgwnkswd ewipesrvlk
       61 yddinlqkkk eldeaynemk kkkpepdpee eidlddssvs seekprkrke sgggrqrrkr
      121 srrdtqvepg etslsreefk vcipdelkpw llddwylvtq qkmlvqvpcs vtvdqilady
      181 vtektsqpnt sshqaritev tngfkkyfnv mlgsqllykf erpqyadile errgtpmsqi
      241 ygsihllrlf vrldsmlktv plekasvenl laevedflqy larnshqlft fddystatre
      301 yirral
//
LOCUS       XP_077506365             335 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115747 [Amblyomma americanum].
ACCESSION   XP_077506365
VERSION     XP_077506365.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650239.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..335
                     /product="uncharacterized protein LOC144115747"
                     /calculated_mol_wt=36339
     Region          <91..>125
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     CDS             1..335
                     /gene="LOC144115747"
                     /coded_by="XM_077650239.1:74..1081"
                     /db_xref="GeneID:144115747"
ORIGIN      
        1 mvfdpdtknv kvkllwlpry mehrriveal epygtvqsve rekwrcpgme hmetanrels
       61 ltlkdgvsas tiphtlnvyg vqalvlipgr pplclrcsrv ghvrrqcrtp rctqcrrfgh
      121 taencvlsya drlrqgqwsr eddvasehim dvsevvdatg elshehridg eqktstpqnn
      181 teyeatghla stppgrkppd pgppvsvtet avlaaqehlv atgqrpdmpp vesveaiqpv
      241 narrsssdad sasslegnge tcvapisgvs gslsavsvaa sdvfpsgswa ealavseedm
      301 dstaplkrpa dadeehpccr saaqrsrssg mkfpa
//
LOCUS       XP_077506366             187 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115748 [Amblyomma americanum].
ACCESSION   XP_077506366
VERSION     XP_077506366.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650240.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..187
                     /product="uncharacterized protein LOC144115748"
                     /calculated_mol_wt=21194
     CDS             1..187
                     /gene="LOC144115748"
                     /coded_by="XM_077650240.1:531..1094"
                     /db_xref="GeneID:144115748"
ORIGIN      
        1 mrerrkikar sgaspdelyq pkwahykslm fldtscssqp sfstvhtqee eeeterqceh
       61 wnnesadqem dfpcssqnca epslpaqqig egtsrpgkkr rrnddeteqr kvllttavet
      121 lqrsqqrlaa ndecdafgsm mahavrqipp gpdrqlamln ahqaivkinl srhsvpveml
      181 nvlnmqe
//
LOCUS       XP_077506367             215 aa            linear   INV 25-NOV-2025
DEFINITION  polyisoprenoid diphosphate/phosphate phosphohydrolase PLPP6-like
            [Amblyomma americanum].
ACCESSION   XP_077506367
VERSION     XP_077506367.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650241.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..215
                     /product="polyisoprenoid diphosphate/phosphate
                     phosphohydrolase PLPP6-like"
                     /calculated_mol_wt=23662
     Region          26..182
                     /region_name="PAP2_like"
                     /note="PAP2_like proteins, a super-family of histidine
                     phosphatases and vanadium haloperoxidases, includes type 2
                     phosphatidic acid phosphatase or lipid phosphate
                     phosphatase (LPP), Glucose-6-phosphatase,
                     Phosphatidylglycerophosphatase B and bacterial acid...;
                     cl00474"
                     /db_xref="CDD:469784"
     Site            order(91,98,119..121,157,163,167)
                     /site_type="active"
                     /db_xref="CDD:238813"
     CDS             1..215
                     /gene="LOC144115749"
                     /coded_by="XM_077650241.1:144..791"
                     /db_xref="GeneID:144115749"
ORIGIN      
        1 msswkqhpvl vrlkaadeql ssglflaark dsplaqyrpl mqgleysahg ipwllgtctv
       61 iwfvsdrele afhvnlllal vldliavaci kaaarrkrpl anrndmfftv svdnhsfpsg
      121 hasrviflac lflncttlni vlkaillaws lsvvvsrvll grhfvgdvtg gailglieyv
      181 iimnifwmgt dtalsfasyf ttfdslhggi kevev
//
LOCUS       XP_077506368             215 aa            linear   INV 25-NOV-2025
DEFINITION  polyisoprenoid diphosphate/phosphate phosphohydrolase PLPP6-like
            [Amblyomma americanum].
ACCESSION   XP_077506368
VERSION     XP_077506368.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650242.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..215
                     /product="polyisoprenoid diphosphate/phosphate
                     phosphohydrolase PLPP6-like"
                     /calculated_mol_wt=23662
     Region          26..182
                     /region_name="PAP2_like"
                     /note="PAP2_like proteins, a super-family of histidine
                     phosphatases and vanadium haloperoxidases, includes type 2
                     phosphatidic acid phosphatase or lipid phosphate
                     phosphatase (LPP), Glucose-6-phosphatase,
                     Phosphatidylglycerophosphatase B and bacterial acid...;
                     cl00474"
                     /db_xref="CDD:469784"
     Site            order(91,98,119..121,157,163,167)
                     /site_type="active"
                     /db_xref="CDD:238813"
     CDS             1..215
                     /gene="LOC144115749"
                     /coded_by="XM_077650242.1:132..779"
                     /db_xref="GeneID:144115749"
ORIGIN      
        1 msswkqhpvl vrlkaadeql ssglflaark dsplaqyrpl mqgleysahg ipwllgtctv
       61 iwfvsdrele afhvnlllal vldliavaci kaaarrkrpl anrndmfftv svdnhsfpsg
      121 hasrviflac lflncttlni vlkaillaws lsvvvsrvll grhfvgdvtg gailglieyv
      181 iimnifwmgt dtalsfasyf ttfdslhggi kevev
//
LOCUS       XP_077506369             215 aa            linear   INV 25-NOV-2025
DEFINITION  polyisoprenoid diphosphate/phosphate phosphohydrolase PLPP6-like
            [Amblyomma americanum].
ACCESSION   XP_077506369
VERSION     XP_077506369.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650243.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..215
                     /product="polyisoprenoid diphosphate/phosphate
                     phosphohydrolase PLPP6-like"
                     /calculated_mol_wt=23662
     Region          26..182
                     /region_name="PAP2_like"
                     /note="PAP2_like proteins, a super-family of histidine
                     phosphatases and vanadium haloperoxidases, includes type 2
                     phosphatidic acid phosphatase or lipid phosphate
                     phosphatase (LPP), Glucose-6-phosphatase,
                     Phosphatidylglycerophosphatase B and bacterial acid...;
                     cl00474"
                     /db_xref="CDD:469784"
     Site            order(91,98,119..121,157,163,167)
                     /site_type="active"
                     /db_xref="CDD:238813"
     CDS             1..215
                     /gene="LOC144115749"
                     /coded_by="XM_077650243.1:63..710"
                     /db_xref="GeneID:144115749"
ORIGIN      
        1 msswkqhpvl vrlkaadeql ssglflaark dsplaqyrpl mqgleysahg ipwllgtctv
       61 iwfvsdrele afhvnlllal vldliavaci kaaarrkrpl anrndmfftv svdnhsfpsg
      121 hasrviflac lflncttlni vlkaillaws lsvvvsrvll grhfvgdvtg gailglieyv
      181 iimnifwmgt dtalsfasyf ttfdslhggi kevev
//
LOCUS       XP_077506370             903 aa            linear   INV 25-NOV-2025
DEFINITION  chromosome transmission fidelity protein 18 homolog isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077506370
VERSION     XP_077506370.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650244.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..903
                     /product="chromosome transmission fidelity protein 18
                     homolog isoform X1"
                     /calculated_mol_wt=101310
     Region          259..>645
                     /region_name="PRK04195"
                     /note="replication factor C large subunit; Provisional"
                     /db_xref="CDD:235250"
     Region          <795..862
                     /region_name="PRK04654"
                     /note="sec-independent translocase; Provisional"
                     /db_xref="CDD:135173"
     CDS             1..903
                     /gene="cutlet"
                     /coded_by="XM_077650244.1:183..2894"
                     /db_xref="GeneID:144115750"
ORIGIN      
        1 mdyddydeye dlyadelala delhsheppa qpskrvlhgr ddfseqdtpr ssssmakrtl
       61 edmagssler attptlgsar kklrleddas ncafefsspk kliideggqg aecatpgast
      121 aqeedlatlp vpsppplast eeelfqtqwq dassyvltig lrgsngrlkf vglrdnswad
      181 dvelllpnnr hkgsllsrpf wdvwndaqae lkrqqlvele rtanitnsha vnslpegvvv
      241 ghsggsnpge tssastslwv ekyrplqfie llsddgvnrt llqwlklwdc vvfgrpqkvr
      301 lqqerhmtan nrssfvkryi pevkeeldeh krpqqkvvll ygppglgktt lahviarhag
      361 ynvvelnasd drspevfkta letatqmkav lgqdprpncl videidgapp asinvlvnmi
      421 kctgqstgkk kkkegslllr pvicicnely vpalrplrqv almlhfpstq kgrlcnrlme
      481 vmrrermkgd qtavaslcek agndvrscls tlqfihgrkk qltltdvrti nvgqkdvqrg
      541 lfsllqevfq rprtgrkvfr hnlnedafgk avknpdgerf dslvrsaqgf gdyekviqal
      601 fdnyvnvsfk dprfeliheg pewlcftdel latvhrlqny slypylpfva pafhrvfsvv
      661 pytkmtfqns fieartkrtq mhnilvsltv estpqarcfl tetsllrdvl pwllcilqpt
      721 lrpvnmqlfn kaerqqleqv vavmssfait yqqtrnadgv yqyslepnie evvhfpgiap
      781 qqslpyatkq iiarelevek mrrsdhlekp ggstagaaps sapvaqpapk vveqparpsm
      841 dffgrviqkp tvtavaaptt rhddqdltyd dptttrtrta ppprlcgdps tldlrlwvtd
      901 rwt
//
LOCUS       XP_077506371             892 aa            linear   INV 25-NOV-2025
DEFINITION  chromosome transmission fidelity protein 18 homolog isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077506371
VERSION     XP_077506371.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650245.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..892
                     /product="chromosome transmission fidelity protein 18
                     homolog isoform X2"
                     /calculated_mol_wt=99985
     Region          259..>645
                     /region_name="PRK04195"
                     /note="replication factor C large subunit; Provisional"
                     /db_xref="CDD:235250"
     CDS             1..892
                     /gene="cutlet"
                     /coded_by="XM_077650245.1:183..2861"
                     /db_xref="GeneID:144115750"
ORIGIN      
        1 mdyddydeye dlyadelala delhsheppa qpskrvlhgr ddfseqdtpr ssssmakrtl
       61 edmagssler attptlgsar kklrleddas ncafefsspk kliideggqg aecatpgast
      121 aqeedlatlp vpsppplast eeelfqtqwq dassyvltig lrgsngrlkf vglrdnswad
      181 dvelllpnnr hkgsllsrpf wdvwndaqae lkrqqlvele rtanitnsha vnslpegvvv
      241 ghsggsnpge tssastslwv ekyrplqfie llsddgvnrt llqwlklwdc vvfgrpqkvr
      301 lqqerhmtan nrssfvkryi pevkeeldeh krpqqkvvll ygppglgktt lahviarhag
      361 ynvvelnasd drspevfkta letatqmkav lgqdprpncl videidgapp asinvlvnmi
      421 kctgqstgkk kkkegslllr pvicicnely vpalrplrqv almlhfpstq kgrlcnrlme
      481 vmrrermkgd qtavaslcek agndvrscls tlqfihgrkk qltltdvrti nvgqkdvqrg
      541 lfsllqevfq rprtgrkvfr hnlnedafgk avknpdgerf dslvrsaqgf gdyekviqal
      601 fdnyvnvsfk dprfeliheg pewlcftdel latvhrlqny slypylpfva pafhrvfsvv
      661 pytkmtfqns fieartkrtq mhnilvsltv estpqarcfl tetsllrdvl pwllcilqpt
      721 lrpvnmqlfn kaerqqleqv vavmssfait yqqtrnadgv yqyslepnie evvhfpgiap
      781 qqslpyatkq iiarelevek mrrsdhlekp ggstagaaps sapvaqpapk vveqparpsm
      841 dffgrviqkp tvtavaetss vgkvssspqv wyqfkegfsn avrrtvrikd ll
//
LOCUS       XP_077506372             346 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115751 [Amblyomma americanum].
ACCESSION   XP_077506372
VERSION     XP_077506372.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650246.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..346
                     /product="uncharacterized protein LOC144115751"
                     /calculated_mol_wt=38714
     Region          73..124
                     /region_name="HTH_Tnp_4"
                     /note="Helix-turn-helix of DDE superfamily endonuclease;
                     pfam13613"
                     /db_xref="CDD:463934"
     Region          155..323
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..346
                     /gene="LOC144115751"
                     /coded_by="XM_077650246.1:344..1384"
                     /db_xref="GeneID:144115751"
ORIGIN      
        1 mtmqdiqale qhsvvlnehl ytlqkekeql evtedslksc eakvrlytgm pnfavlfavf
       61 ealasfishn vnnkltkfqe fvlfmmklkv nlqnadlafr fniseatvsr ifdkwlhvay
      121 crlkdeiiwp trdalqktmp qsfydsfgan vadiidcfei kierpssylp rcetwsqykg
      181 sntakfliai apqgvvtyis egwggrasdk hitehcgfld nlvpgdvvla drgfnisesv
      241 gfycaklhvp aftrgkkqls aedvqstrnl anvrihverv iglirnkfif lksvvpidyv
      301 vcrpgdevap ldkivtvccv lsnlcasiva apkdatgqqa sasvde
//
LOCUS       XP_077506373             113 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115752 [Amblyomma americanum].
ACCESSION   XP_077506373
VERSION     XP_077506373.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650247.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..113
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..113
                     /product="uncharacterized protein LOC144115752"
                     /calculated_mol_wt=12431
     CDS             1..113
                     /gene="LOC144115752"
                     /coded_by="XM_077650247.1:300..641"
                     /db_xref="GeneID:144115752"
ORIGIN      
        1 maaeelkeqe vevlmewetd ssgtisaeee mpipvsppmr kkpchrqlpf drnqsdreaa
       61 vaglrqgtgn aqatarpslq arrvavmvrr llditcihsk qqleesgtpv ipv
//
LOCUS       XP_077506374             240 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115754 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506374
VERSION     XP_077506374.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650248.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..240
                     /product="uncharacterized protein LOC144115754 isoform X1"
                     /calculated_mol_wt=24334
     CDS             1..240
                     /gene="LOC144115754"
                     /coded_by="XM_077650248.1:3170..3892"
                     /db_xref="GeneID:144115754"
ORIGIN      
        1 maraqaifla hnaavaahta gpggcpgvgp aglctplasc ggdgihvwpe tsctwppvgl
       61 laglvqnlpa rlevqflavq ealqkdaagp paaaavqlwp qvpchttsap smlvvaglps
      121 llppafstpl pqalsisvga rssvaishas alvgavssae rralkhgvrs pccsgggssd
      181 edllldvese rdrfsgpsgs pnasqersrl calhrskldp vrpqvmpplc eecvfldaal
//
LOCUS       XP_077506375             240 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115754 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506375
VERSION     XP_077506375.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650249.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..240
                     /product="uncharacterized protein LOC144115754 isoform X1"
                     /calculated_mol_wt=24334
     CDS             1..240
                     /gene="LOC144115754"
                     /coded_by="XM_077650249.1:2191..2913"
                     /db_xref="GeneID:144115754"
ORIGIN      
        1 maraqaifla hnaavaahta gpggcpgvgp aglctplasc ggdgihvwpe tsctwppvgl
       61 laglvqnlpa rlevqflavq ealqkdaagp paaaavqlwp qvpchttsap smlvvaglps
      121 llppafstpl pqalsisvga rssvaishas alvgavssae rralkhgvrs pccsgggssd
      181 edllldvese rdrfsgpsgs pnasqersrl calhrskldp vrpqvmpplc eecvfldaal
//
LOCUS       XP_077506376             202 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115754 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506376
VERSION     XP_077506376.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650250.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..202
                     /product="uncharacterized protein LOC144115754 isoform X2"
                     /calculated_mol_wt=21419
     CDS             1..202
                     /gene="LOC144115754"
                     /coded_by="XM_077650250.1:131..739"
                     /db_xref="GeneID:144115754"
ORIGIN      
        1 mthnhapkft spswrhfyar tllrpphlrk qarlevqfla vqealqkdaa gppaaaavql
       61 wpqvpchtts apsmlvvagl psllppafst plpqalsisv garssvaish asalvgavss
      121 aerralkhgv rspccsgggs sdedllldve serdrfsgps gspnasqers rlcalhrskl
      181 dpvrpqvmpp lceecvflda al
//
LOCUS       XP_077506377             202 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115754 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506377
VERSION     XP_077506377.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650251.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..202
                     /product="uncharacterized protein LOC144115754 isoform X2"
                     /calculated_mol_wt=21419
     CDS             1..202
                     /gene="LOC144115754"
                     /coded_by="XM_077650251.1:241..849"
                     /db_xref="GeneID:144115754"
ORIGIN      
        1 mthnhapkft spswrhfyar tllrpphlrk qarlevqfla vqealqkdaa gppaaaavql
       61 wpqvpchtts apsmlvvagl psllppafst plpqalsisv garssvaish asalvgavss
      121 aerralkhgv rspccsgggs sdedllldve serdrfsgps gspnasqers rlcalhrskl
      181 dpvrpqvmpp lceecvflda al
//
LOCUS       XP_077506378             420 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115756 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506378
VERSION     XP_077506378.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650252.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..420
                     /product="uncharacterized protein LOC144115756 isoform X1"
                     /calculated_mol_wt=45465
     Region          <118..>170
                     /region_name="APC10-like"
                     /note="APC10-like DOC1 domains in E3 ubiquitin ligases
                     that mediate substrate ubiquitination; cl02148"
                     /db_xref="CDD:382862"
     Site            order(130,157,164,166)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176482"
     CDS             1..420
                     /gene="LOC144115756"
                     /coded_by="XM_077650252.1:211..1473"
                     /db_xref="GeneID:144115756"
ORIGIN      
        1 msgihrdrrq ragspkrepk psgrprpssq cmasvgfett gstlagnrvd cccvpsafis
       61 tarrsptray irsvwrrsap rqlprrslai wwaaaashqh sfppqgdllr ahifgvsgvg
      121 rlrnnwldar wqssglqlpl isiqfhckvt iqgicicady mrgescnpnr rfgsvhrhsq
      181 pqatavrrdv apeptppvhf chpalqspas pppspqgqen sevtcaddtt pkaagradtd
      241 hgcfevltsr ppvglqpsdi tgavtplcsl iqqhqaafva htsearklvd ecaaanvstl
      301 sllqstvqgl qeqqtaqmat glmgpghtrl avttgrcspl qmhrqapaev wdsattspqf
      361 pspphlltra rvspvvrlap rhclrtvtdc seettcstih gttalreelf adpvlhvyga
//
LOCUS       XP_077506379             420 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115756 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506379
VERSION     XP_077506379.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650253.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..420
                     /product="uncharacterized protein LOC144115756 isoform X1"
                     /calculated_mol_wt=45465
     Region          <118..>170
                     /region_name="APC10-like"
                     /note="APC10-like DOC1 domains in E3 ubiquitin ligases
                     that mediate substrate ubiquitination; cl02148"
                     /db_xref="CDD:382862"
     Site            order(130,157,164,166)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176482"
     CDS             1..420
                     /gene="LOC144115756"
                     /coded_by="XM_077650253.1:201..1463"
                     /db_xref="GeneID:144115756"
ORIGIN      
        1 msgihrdrrq ragspkrepk psgrprpssq cmasvgfett gstlagnrvd cccvpsafis
       61 tarrsptray irsvwrrsap rqlprrslai wwaaaashqh sfppqgdllr ahifgvsgvg
      121 rlrnnwldar wqssglqlpl isiqfhckvt iqgicicady mrgescnpnr rfgsvhrhsq
      181 pqatavrrdv apeptppvhf chpalqspas pppspqgqen sevtcaddtt pkaagradtd
      241 hgcfevltsr ppvglqpsdi tgavtplcsl iqqhqaafva htsearklvd ecaaanvstl
      301 sllqstvqgl qeqqtaqmat glmgpghtrl avttgrcspl qmhrqapaev wdsattspqf
      361 pspphlltra rvspvvrlap rhclrtvtdc seettcstih gttalreelf adpvlhvyga
//
LOCUS       XP_077506380             339 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115756 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506380
VERSION     XP_077506380.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650254.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..339
                     /product="uncharacterized protein LOC144115756 isoform X2"
                     /calculated_mol_wt=36328
     Region          9..>89
                     /region_name="APC10-like"
                     /note="APC10-like DOC1 domains in E3 ubiquitin ligases
                     that mediate substrate ubiquitination; cl02148"
                     /db_xref="CDD:382862"
     Site            order(49,76,83,85)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176482"
     CDS             1..339
                     /gene="LOC144115756"
                     /coded_by="XM_077650254.1:199..1218"
                     /db_xref="GeneID:144115756"
ORIGIN      
        1 mssgyvrhps rsetkgrvpe agaqavwsas sqqpvsgvgr lrnnwldarw qssglqlpli
       61 siqfhckvti qgicicadym rgescnpnrr fgsvhrhsqp qatavrrdva peptppvhfc
      121 hpalqspasp ppspqgqens evtcaddttp kaagradtdh gcfevltsrp pvglqpsdit
      181 gavtplcsli qqhqaafvah tsearklvde caaanvstls llqstvqglq eqqtaqmatg
      241 lmgpghtrla vttgrcsplq mhrqapaevw dsattspqfp spphlltrar vspvvrlapr
      301 hclrtvtdcs eettcstihg ttalreelfa dpvlhvyga
//
LOCUS       XP_077506381             339 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115756 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506381
VERSION     XP_077506381.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650255.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..339
                     /product="uncharacterized protein LOC144115756 isoform X2"
                     /calculated_mol_wt=36328
     Region          9..>89
                     /region_name="APC10-like"
                     /note="APC10-like DOC1 domains in E3 ubiquitin ligases
                     that mediate substrate ubiquitination; cl02148"
                     /db_xref="CDD:382862"
     Site            order(49,76,83,85)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176482"
     CDS             1..339
                     /gene="LOC144115756"
                     /coded_by="XM_077650255.1:191..1210"
                     /db_xref="GeneID:144115756"
ORIGIN      
        1 mssgyvrhps rsetkgrvpe agaqavwsas sqqpvsgvgr lrnnwldarw qssglqlpli
       61 siqfhckvti qgicicadym rgescnpnrr fgsvhrhsqp qatavrrdva peptppvhfc
      121 hpalqspasp ppspqgqens evtcaddttp kaagradtdh gcfevltsrp pvglqpsdit
      181 gavtplcsli qqhqaafvah tsearklvde caaanvstls llqstvqglq eqqtaqmatg
      241 lmgpghtrla vttgrcsplq mhrqapaevw dsattspqfp spphlltrar vspvvrlapr
      301 hclrtvtdcs eettcstihg ttalreelfa dpvlhvyga
//
LOCUS       XP_077506382             420 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115758 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506382
VERSION     XP_077506382.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650256.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..420
                     /product="uncharacterized protein LOC144115758 isoform X1"
                     /calculated_mol_wt=45421
     Region          <118..>170
                     /region_name="APC10-like"
                     /note="APC10-like DOC1 domains in E3 ubiquitin ligases
                     that mediate substrate ubiquitination; cl02148"
                     /db_xref="CDD:382862"
     CDS             1..420
                     /gene="LOC144115758"
                     /coded_by="XM_077650256.1:433..1695"
                     /db_xref="GeneID:144115758"
ORIGIN      
        1 msgihrdrrq ragspkrepk psgrprpssq clasvgfett gstlagnrvd cscvpsafis
       61 ttrrsptray irsvwrrsap rqlprrslai wraaaashqh sfppqgdllr ahifavsgvg
      121 rlrnncldar wpssrlqlpp isiqfhckvt iqgicicady mrgescnpnr rfgsvhrhsq
      181 pqatavrrdv apeptppvhf chpalqspas pppspqgqen sevtcaddtt pkaagradtd
      241 hgcfevltsr ppvglqpsdi tgavtplcsl iqqhqaafva htsearklvd ecaaanvstl
      301 sllqstvqgl qeqqtaqmat glmgpghtrl avttgrcspl qmhrqapaev whsattsplf
      361 pspphlltra rvspvvrlap rhclrtvtdc seettcstih gttalreelf adpvlhvyga
//
LOCUS       XP_077506384             419 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115758 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506384
VERSION     XP_077506384.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650258.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..419
                     /product="uncharacterized protein LOC144115758 isoform X2"
                     /calculated_mol_wt=45350
     Region          <118..>170
                     /region_name="APC10-like"
                     /note="APC10-like DOC1 domains in E3 ubiquitin ligases
                     that mediate substrate ubiquitination; cl02148"
                     /db_xref="CDD:382862"
     CDS             1..419
                     /gene="LOC144115758"
                     /coded_by="XM_077650258.1:439..1698"
                     /db_xref="GeneID:144115758"
ORIGIN      
        1 msgihrdrrq ragspkrepk psgrprpssq clasvgfett gstlagnrvd cscvpsafis
       61 ttrrsptray irsvwrrsap rqlprrslai wraaaashqh sfppqgdllr ahifavsgvg
      121 rlrnncldar wpssrlqlpp isiqfhckvt iqgicicady mrgescnpnr rfgsvhrhsq
      181 pqatavrrdv apeptppvhf chpalqspas pppspqgqen sevtcaddtt pkagradtdh
      241 gcfevltsrp pvglqpsdit gavtplcsli qqhqaafvah tsearklvde caaanvstls
      301 llqstvqglq eqqtaqmatg lmgpghtrla vttgrcsplq mhrqapaevw hsattsplfp
      361 spphlltrar vspvvrlapr hclrtvtdcs eettcstihg ttalreelfa dpvlhvyga
//
LOCUS       XP_077506385             362 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115758 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077506385
VERSION     XP_077506385.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650259.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..362
                     /product="uncharacterized protein LOC144115758 isoform X3"
                     /calculated_mol_wt=38762
     Region          32..>112
                     /region_name="APC10-like"
                     /note="APC10-like DOC1 domains in E3 ubiquitin ligases
                     that mediate substrate ubiquitination; cl02148"
                     /db_xref="CDD:382862"
     Site            order(72,99,106,108)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176482"
     CDS             1..362
                     /gene="LOC144115758"
                     /coded_by="XM_077650259.1:122..1210"
                     /db_xref="GeneID:144115758"
ORIGIN      
        1 mqglliwssg airhfgqpcs adtmssgyvr hpsrsetkgr vpeagaqavw sassqqpvsg
       61 vgrlrnncld arwpssrlql ppisiqfhck vtiqgicica dymrgescnp nrrfgsvhrh
      121 sqpqatavrr dvapeptppv hfchpalqsp aspppspqgq ensevtcadd ttpkaagrad
      181 tdhgcfevlt srppvglqps ditgavtplc sliqqhqaaf vahtsearkl vdecaaanvs
      241 tlsllqstvq glqeqqtaqm atglmgpght rlavttgrcs plqmhrqapa evwhsattsp
      301 lfpspphllt rarvspvvrl aprhclrtvt dcseettcst ihgttalree lfadpvlhvy
      361 ga
//
LOCUS       XP_077506386              75 aa            linear   INV 25-NOV-2025
DEFINITION  actin-binding LIM protein 2-like [Amblyomma americanum].
ACCESSION   XP_077506386
VERSION     XP_077506386.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650260.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..75
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..75
                     /product="actin-binding LIM protein 2-like"
                     /calculated_mol_wt=9181
     Region          40..75
                     /region_name="VHP"
                     /note="Villin headpiece domain; pfam02209"
                     /db_xref="CDD:460493"
     CDS             1..75
                     /gene="LOC144115759"
                     /coded_by="XM_077650260.1:117..344"
                     /db_xref="GeneID:144115759"
ORIGIN      
        1 mpnvnwhlst dppklypyhl lmtsnyrlpv dvdrchlerh lsneefqalf hmsrlefyrl
       61 pewkrndlkr raklf
//
LOCUS       XP_077506387             363 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115760 [Amblyomma americanum].
ACCESSION   XP_077506387
VERSION     XP_077506387.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650261.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 25% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..363
                     /product="uncharacterized protein LOC144115760"
                     /calculated_mol_wt=40527
     Region          66..131
                     /region_name="GT1"
                     /note="myb-like, SANT family; cl23759"
                     /db_xref="CDD:474047"
     CDS             1..363
                     /gene="LOC144115760"
                     /coded_by="XM_077650261.1:1..1092"
                     /db_xref="GeneID:144115760"
ORIGIN      
        1 mrrwqrlppp qdalaaagra cadgsdrakl deciaalpsh leearrtles laqhycanea
       61 wkiddvsila kyqtivenkr tngvsldkkk ktwkeitdey ncrpnvrfrt eaqlrkcwdn
      121 lkekwrkgka ddmkevfttg ggsapatqlt delervgaaa shmstrvenp fdsdrgrhav
      181 tplskstpsv vsllqpmqve slepsndsiy qwdnpaspnn essqvaciqe lqnettaaev
      241 elsaerepad lqpavpqlas plqaaplqaa plqetaqhpa paqrrmltkr aaveaelsar
      301 lcsvdedrkr krrehllkmk lmraehalhm khskqlhele catkkaklel lmlkiaavkk
      361 ask
//
LOCUS       XP_077506388             101 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115762 [Amblyomma americanum].
ACCESSION   XP_077506388
VERSION     XP_077506388.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650262.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..101
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..101
                     /product="uncharacterized protein LOC144115762"
                     /calculated_mol_wt=10551
     Region          53..>72
                     /region_name="PWWP"
                     /note="PWWP (Pro-Trp-Trp-Pro) domain; cl02554"
                     /db_xref="CDD:470613"
     Site            65..68
                     /site_type="other"
                     /note="PWWP sequence motif"
                     /db_xref="CDD:438958"
     CDS             1..101
                     /gene="LOC144115762"
                     /coded_by="XM_077650262.1:691..996"
                     /db_xref="GeneID:144115762"
ORIGIN      
        1 merkrhlgsr tqpqraapmq aiaatassar sdpeagspeg tlgalgqpcn csvgalvwar
       61 lgsarrwpav vvtefpfvar dqlhlaacgs agwlstespr r
//
LOCUS       XP_077506389             477 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115764 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506389
VERSION     XP_077506389.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650263.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..477
                     /product="uncharacterized protein LOC144115764 isoform X1"
                     /calculated_mol_wt=51944
     Region          <118..>170
                     /region_name="APC10-like"
                     /note="APC10-like DOC1 domains in E3 ubiquitin ligases
                     that mediate substrate ubiquitination; cl02148"
                     /db_xref="CDD:382862"
     Site            order(130,157,164,166)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176482"
     CDS             1..477
                     /gene="LOC144115764"
                     /coded_by="XM_077650263.1:211..1644"
                     /db_xref="GeneID:144115764"
ORIGIN      
        1 msgihrdrrq ragspkrepk psgrprpssq cmasvgfett gstlagnrvd cccvpsafis
       61 tarrsptray irsvwrrsap rqlprrslai wwaaaashqh sfppqgdllr ahifgvsgvg
      121 rlrnnwldar wqssglqlpl isiqfhckvt iqgicicady mrgescnpnr rfgsvhrhsq
      181 pqatavrrdv apeptppvhf chpalqspas pppspqgqen sevtcaddtt pkaagradtd
      241 hgcfevltsr ppvglqpsdi tgavtplcsl iqqhqaafva htsearklvd ecaaanvstl
      301 sllqstvqgl qeqqtaqmat glmdpghtrl avttgrcspl qmhrqdgttg nadistssgg
      361 qcsrlpvvpd lfspdftnyr elfhqdllef yhnsvrflvt avapaevwdr attspqfpsp
      421 phlltrarvs pvvrlaprhc lrtvtdcsee ttcstihgtt alreelfadp vfhvyga
//
LOCUS       XP_077506390             477 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115764 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506390
VERSION     XP_077506390.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650264.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..477
                     /product="uncharacterized protein LOC144115764 isoform X1"
                     /calculated_mol_wt=51944
     Region          <118..>170
                     /region_name="APC10-like"
                     /note="APC10-like DOC1 domains in E3 ubiquitin ligases
                     that mediate substrate ubiquitination; cl02148"
                     /db_xref="CDD:382862"
     Site            order(130,157,164,166)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176482"
     CDS             1..477
                     /gene="LOC144115764"
                     /coded_by="XM_077650264.1:202..1635"
                     /db_xref="GeneID:144115764"
ORIGIN      
        1 msgihrdrrq ragspkrepk psgrprpssq cmasvgfett gstlagnrvd cccvpsafis
       61 tarrsptray irsvwrrsap rqlprrslai wwaaaashqh sfppqgdllr ahifgvsgvg
      121 rlrnnwldar wqssglqlpl isiqfhckvt iqgicicady mrgescnpnr rfgsvhrhsq
      181 pqatavrrdv apeptppvhf chpalqspas pppspqgqen sevtcaddtt pkaagradtd
      241 hgcfevltsr ppvglqpsdi tgavtplcsl iqqhqaafva htsearklvd ecaaanvstl
      301 sllqstvqgl qeqqtaqmat glmdpghtrl avttgrcspl qmhrqdgttg nadistssgg
      361 qcsrlpvvpd lfspdftnyr elfhqdllef yhnsvrflvt avapaevwdr attspqfpsp
      421 phlltrarvs pvvrlaprhc lrtvtdcsee ttcstihgtt alreelfadp vfhvyga
//
LOCUS       XP_077506391             420 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115764 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506391
VERSION     XP_077506391.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650265.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..420
                     /product="uncharacterized protein LOC144115764 isoform X2"
                     /calculated_mol_wt=45626
     Region          <118..>170
                     /region_name="APC10-like"
                     /note="APC10-like DOC1 domains in E3 ubiquitin ligases
                     that mediate substrate ubiquitination; cl02148"
                     /db_xref="CDD:382862"
     Site            order(130,157,164,166)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176482"
     CDS             1..420
                     /gene="LOC144115764"
                     /coded_by="XM_077650265.1:4022..5284"
                     /db_xref="GeneID:144115764"
ORIGIN      
        1 msgihrdrrq ragspkrepk psgrprpssq cmasvgfett gstlagnrvd cccvpsafis
       61 tarrsptray irsvwrrsap rqlprrslai wwaaaashqh sfppqgdllr ahifgvsgvg
      121 rlrnnwldar wqssglqlpl isiqfhckvt iqgicicady mrgescnpnr rfgsvhrhsq
      181 pqatavrrdv apeptppvhf chpalqspas pppspqgqen sevtcaddtt pkaagradtd
      241 hgcfevltsr ppvglqpsdi tgavtplcsl iqqhqaafva htsearklvd ecaaanvstl
      301 sllqstvqgl qeqqtaqmat glmdpghtrl avttgrcspl qmhrqapaev wdrattspqf
      361 pspphlltra rvspvvrlap rhclrtvtdc seettcstih gttalreelf adpvfhvyga
//
LOCUS       XP_077506392             396 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115764 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077506392
VERSION     XP_077506392.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650266.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..396
                     /product="uncharacterized protein LOC144115764 isoform X3"
                     /calculated_mol_wt=42807
     Region          9..>89
                     /region_name="APC10-like"
                     /note="APC10-like DOC1 domains in E3 ubiquitin ligases
                     that mediate substrate ubiquitination; cl02148"
                     /db_xref="CDD:382862"
     Site            order(49,76,83,85)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176482"
     CDS             1..396
                     /gene="LOC144115764"
                     /coded_by="XM_077650266.1:201..1391"
                     /db_xref="GeneID:144115764"
ORIGIN      
        1 mssgyvrhps rsetkgrvpe agaqavwsas sqqpvsgvgr lrnnwldarw qssglqlpli
       61 siqfhckvti qgicicadym rgescnpnrr fgsvhrhsqp qatavrrdva peptppvhfc
      121 hpalqspasp ppspqgqens evtcaddttp kaagradtdh gcfevltsrp pvglqpsdit
      181 gavtplcsli qqhqaafvah tsearklvde caaanvstls llqstvqglq eqqtaqmatg
      241 lmdpghtrla vttgrcsplq mhrqdgttgn adistssggq csrlpvvpdl fspdftnyre
      301 lfhqdllefy hnsvrflvta vapaevwdra ttspqfpspp hlltrarvsp vvrlaprhcl
      361 rtvtdcseet tcstihgtta lreelfadpv fhvyga
//
LOCUS       XP_077506393             396 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115764 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077506393
VERSION     XP_077506393.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650267.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..396
                     /product="uncharacterized protein LOC144115764 isoform X3"
                     /calculated_mol_wt=42807
     Region          9..>89
                     /region_name="APC10-like"
                     /note="APC10-like DOC1 domains in E3 ubiquitin ligases
                     that mediate substrate ubiquitination; cl02148"
                     /db_xref="CDD:382862"
     Site            order(49,76,83,85)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176482"
     CDS             1..396
                     /gene="LOC144115764"
                     /coded_by="XM_077650267.1:192..1382"
                     /db_xref="GeneID:144115764"
ORIGIN      
        1 mssgyvrhps rsetkgrvpe agaqavwsas sqqpvsgvgr lrnnwldarw qssglqlpli
       61 siqfhckvti qgicicadym rgescnpnrr fgsvhrhsqp qatavrrdva peptppvhfc
      121 hpalqspasp ppspqgqens evtcaddttp kaagradtdh gcfevltsrp pvglqpsdit
      181 gavtplcsli qqhqaafvah tsearklvde caaanvstls llqstvqglq eqqtaqmatg
      241 lmdpghtrla vttgrcsplq mhrqdgttgn adistssggq csrlpvvpdl fspdftnyre
      301 lfhqdllefy hnsvrflvta vapaevwdra ttspqfpspp hlltrarvsp vvrlaprhcl
      361 rtvtdcseet tcstihgtta lreelfadpv fhvyga
//
LOCUS       XP_077506395             391 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115764 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077506395
VERSION     XP_077506395.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650269.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..391
                     /product="uncharacterized protein LOC144115764 isoform X4"
                     /calculated_mol_wt=42249
     Region          <118..>170
                     /region_name="APC10-like"
                     /note="APC10-like DOC1 domains in E3 ubiquitin ligases
                     that mediate substrate ubiquitination; cl02148"
                     /db_xref="CDD:382862"
     Site            order(130,157,164,166)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176482"
     CDS             1..391
                     /gene="LOC144115764"
                     /coded_by="XM_077650269.1:211..1386"
                     /db_xref="GeneID:144115764"
ORIGIN      
        1 msgihrdrrq ragspkrepk psgrprpssq cmasvgfett gstlagnrvd cccvpsafis
       61 tarrsptray irsvwrrsap rqlprrslai wwaaaashqh sfppqgdllr ahifgvsgvg
      121 rlrnnwldar wqssglqlpl isiqfhckvt iqgicicady mrgescnpnr rfgsvhrhsq
      181 pqatavrrdv apeptppvhf chpalqspas pppspqgqen sevtcaddtt pkaagradtd
      241 hgcfevltsr ppvglqpsdi tgavtplcsl iqqhqaafva htsearklvd ecaaanvstl
      301 sllqstvqgl qeqqtaqmat glmdpghtrl avttgrcspl qmhrqvarqm aplamltsap
      361 qvvvnaaacl wcqtcshrts pttgncstri f
//
LOCUS       XP_077506396             386 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115766 [Amblyomma americanum].
ACCESSION   XP_077506396
VERSION     XP_077506396.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650270.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..386
                     /product="uncharacterized protein LOC144115766"
                     /calculated_mol_wt=41767
     Region          <118..>170
                     /region_name="APC10-like"
                     /note="APC10-like DOC1 domains in E3 ubiquitin ligases
                     that mediate substrate ubiquitination; cl02148"
                     /db_xref="CDD:382862"
     Site            order(130,157,164,166)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176482"
     CDS             1..386
                     /gene="LOC144115766"
                     /coded_by="XM_077650270.1:195..1355"
                     /db_xref="GeneID:144115766"
ORIGIN      
        1 msgihrdrrq rtgspkrepk psgrprpssq cmasvgfett gstlagnrvd cccvpsafis
       61 tarrsptray irsvwrrsap rqlprrslai wwaaaashqh sfppqgdllr ahifgvsgvg
      121 rlrnnwldar wqssglqlpl isiqfhckvt iqgicicady mrgescnpnr rfgsvhrhsq
      181 pqatavrrdv apeptppvhf chpalqspas pppspqgqen sevtcaddtt pkaagradtd
      241 hgcfevltsr ppvglqpsdi tgavtplcsl iqqhqaafva htsearklvd ecaaanvstl
      301 sllqstvqgl qeqqtaqmat glmgpghtrl avttgrcspl qmhrqgksgs ysvpllhpli
      361 slmtinngmt kamkkdawcs cniesl
//
LOCUS       XP_077506397             386 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115766 [Amblyomma americanum].
ACCESSION   XP_077506397
VERSION     XP_077506397.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650271.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..386
                     /product="uncharacterized protein LOC144115766"
                     /calculated_mol_wt=41767
     Region          <118..>170
                     /region_name="APC10-like"
                     /note="APC10-like DOC1 domains in E3 ubiquitin ligases
                     that mediate substrate ubiquitination; cl02148"
                     /db_xref="CDD:382862"
     Site            order(130,157,164,166)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176482"
     CDS             1..386
                     /gene="LOC144115766"
                     /coded_by="XM_077650271.1:186..1346"
                     /db_xref="GeneID:144115766"
ORIGIN      
        1 msgihrdrrq rtgspkrepk psgrprpssq cmasvgfett gstlagnrvd cccvpsafis
       61 tarrsptray irsvwrrsap rqlprrslai wwaaaashqh sfppqgdllr ahifgvsgvg
      121 rlrnnwldar wqssglqlpl isiqfhckvt iqgicicady mrgescnpnr rfgsvhrhsq
      181 pqatavrrdv apeptppvhf chpalqspas pppspqgqen sevtcaddtt pkaagradtd
      241 hgcfevltsr ppvglqpsdi tgavtplcsl iqqhqaafva htsearklvd ecaaanvstl
      301 sllqstvqgl qeqqtaqmat glmgpghtrl avttgrcspl qmhrqgksgs ysvpllhpli
      361 slmtinngmt kamkkdawcs cniesl
//
LOCUS       XP_077506398             347 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115767 [Amblyomma americanum].
ACCESSION   XP_077506398
VERSION     XP_077506398.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650272.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..347
                     /product="uncharacterized protein LOC144115767"
                     /calculated_mol_wt=38482
     Region          87..123
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cd00167"
                     /db_xref="CDD:238096"
     CDS             1..347
                     /gene="LOC144115767"
                     /coded_by="XM_077650272.1:184..1227"
                     /db_xref="GeneID:144115767"
ORIGIN      
        1 mmeassdspl stpgtssaes ladetgmsps eppaenetpp sqppakkaka srgprrhllp
       61 rlrssrgrnl klpmhfkseg lkddyknwta kerkqlldal kehgssdlek laravptrsa
      121 tainsflmeh rckkdrivvd gatgrrtaqa lrilrraldv errrvdrsqa lvqvvascqr
      181 qpfteptevd gaelpnfdni yqmlqdvmen kvpaalgpcd qfvvrrllgt lttlvatlnl
      241 neerevlkkq lawaamqptr kdaegprlep gekptltfps msqrkenlks swnplqlpae
      301 viqkpwpain klmaanvfrs erttsqgksa pstaaqgrsr qdpqslq
//
LOCUS       XP_077506399             531 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115768 [Amblyomma americanum].
ACCESSION   XP_077506399
VERSION     XP_077506399.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650273.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..531
                     /product="uncharacterized protein LOC144115768"
                     /calculated_mol_wt=58138
     Region          <174..>349
                     /region_name="PABP-1234"
                     /note="polyadenylate binding protein, human types 1, 2, 3,
                     4 family; TIGR01628"
                     /db_xref="CDD:130689"
     Region          283..351
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cd00590"
                     /db_xref="CDD:409669"
     CDS             1..531
                     /gene="LOC144115768"
                     /coded_by="XM_077650273.1:83..1678"
                     /db_xref="GeneID:144115768"
ORIGIN      
        1 mektldvfcd svawltrgef teedleeakl acfarvdkpi tpgdcgaaaf lhgitdemkq
       61 ehrqrlfsst kddviaavkr caseggctss vavigpenkf tetgrhwlvh rdgsnkpged
      121 ledgkeagqv aeesaahaqk kdssskgdss ssssgsdsdt sdsdrdssad rfllkihdlp
      181 enitaeqvar hlkqagvrvs ggrlgilllt rskgdlpigr afvevasked qeralsyawe
      241 saklsghrik afrgqvddvr ryvmsrrpal salhkgvtss cvvqvrnlpv gateeklkel
      301 fshckvtqve rekfnlgraq raafmrfmcp deaqralavn grklmgqkle lclvpedyad
      361 rllegdllnl asyaeqkgsr igtrrvsgta rraakeaslr egatnfrlae pheleailqt
      421 segqhrvvae glpfsiaqie nflwpakpsl vvrvrrasgv igaivgaivg pvnsvilqlk
      481 finflsaevs gdiyienyyi edvmvrqllf saipfckild vcisartqas y
//
LOCUS       XP_077506400             840 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115769 [Amblyomma americanum].
ACCESSION   XP_077506400
VERSION     XP_077506400.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650274.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..840
                     /product="uncharacterized protein LOC144115769"
                     /calculated_mol_wt=92069
     Region          65..>331
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..840
                     /gene="LOC144115769"
                     /coded_by="XM_077650274.1:197..2719"
                     /db_xref="GeneID:144115769"
ORIGIN      
        1 mpllrlsaar alrendslrf nsccpfcifv nssaacgfvc epamerrtgq khrtkyaygk
       61 rkrkppipkq krpaatephe rrsapissha dvsseaslpl esatssesgg lapsashdia
      121 vqyrpghssv faaasggavp vqrddgevlp kqkhtvqvhl psesitgses vgpapstsrd
      181 iavqyrpghs svfaaasaga vpvqrddgev fpkqkrtvqv hlpsesmtgs esgdpapsts
      241 rdiavqhrpg qssvfaaasd dampaerdds elfpkqkrtv qvhleprfvt leaaeeqacs
      301 vratlravss terkfgfarq teeesapded eftliqasvm nsiigsllcp kcskkrlsvk
      361 hetklglavk lvlscgacgp iashwsskrk segrtfevni ramqaiktig kgptalndfw
      421 atmnvshrgl hqktyqghlk klfkpgaeea aqnifadavl avkdvyskmq psspsnitvv
      481 ydgtwltrgh sshigvgcii efytglvldc tvlsnfclgc cqqpaesdps ygtwieqhqc
      541 qkntdvasgq meveaalilf rrsfssyglr ytnviwdgds rtylalckde vygfipltke
      601 dcinhvqkrm gtalrtlvar akkgeplggk ggltqdlikk ltnyyglalr rntevsdmqr
      661 avmatlyhvt stddephhel cppgpdswcr hrsaqakmep ppphkyklpr rvaeallpvy
      721 qrlsdpqlle rckgnktqna aeslhsviws miskdqhasl favetavhea varynfgnlr
      781 aytevcksvg ikpgslaler aqekdkqrkr kacsaektke qrhkktpask dtkyyspgaf
//
LOCUS       XP_077506401             171 aa            linear   INV 25-NOV-2025
DEFINITION  leucine-rich repeats and immunoglobulin-like domains protein 3
            [Amblyomma americanum].
ACCESSION   XP_077506401
VERSION     XP_077506401.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650275.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..171
                     /product="leucine-rich repeats and immunoglobulin-like
                     domains protein 3"
                     /calculated_mol_wt=18376
     Region          <11..>140
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          14..34
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          36..60
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          62..84
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          85..107
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          108..127
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..171
                     /gene="LOC144115770"
                     /coded_by="XM_077650275.1:994..1509"
                     /db_xref="GeneID:144115770"
ORIGIN      
        1 mvqpgsdvla lanlsvlnlk adgiyalpte msnlplrflt vsenritelq pelfenrlss
       61 cllcldvsnn qltvlpetlc narklmslaa grnrlhklpd glgdlpdlwm ldltsnaltl
      121 lpasllklsw isiwgnplss tagqpmehta aswqvplplt elaaravvav g
//
LOCUS       XP_077506402             538 aa            linear   INV 25-NOV-2025
DEFINITION  protein PALS2-like [Amblyomma americanum].
ACCESSION   XP_077506402
VERSION     XP_077506402.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650276.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..538
                     /product="protein PALS2-like"
                     /calculated_mol_wt=61036
     Region          31..78
                     /region_name="L27"
                     /note="L27 domain; pfam02828"
                     /db_xref="CDD:460717"
     Region          101..178
                     /region_name="PDZ_MPP6-MPP2-like"
                     /note="PDZ domain of membrane palmitoylated protein 6
                     (MPP6), MPP2, and related domains; cd10832"
                     /db_xref="CDD:467268"
     Site            order(112..118,127,130,158,162,165..166)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467268"
     Region          190..249
                     /region_name="SH3_MPP"
                     /note="Src Homology 3 domain of Membrane Protein,
                     Palmitoylated (or MAGUK p55 subfamily member) proteins;
                     cd11862"
                     /db_xref="CDD:212796"
     Site            order(192,216..217)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212796"
     Site            order(195,197,200,211,229..230,246,248..249)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212796"
     Region          311..525
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..538
                     /gene="LOC144115774"
                     /coded_by="XM_077650276.1:337..1953"
                     /db_xref="GeneID:144115774"
ORIGIN      
        1 mespvmsclv qvqdhleekt ttfkpvstnn vellrdvcqa crrvsgrdaq elanilsgph
       61 fsallnahda vatksyepak plalekeapa mapetlndti rmvgirkndd eplgitvrve
      121 ddnlmiaril aggiidrqgl lhvgdtilev ngvpvhtpeq lqlqlkkaqg svafkilpsy
      181 hdnlptfqcf vkalytyeps kdtllpckei glpfkqgdil qvlnqedpnw wqarrvsasg
      241 paglipsqel eerrrafvrp eydyatktkm cgtkvtkkkr ktmyeskasa efekaelllf
      301 eevarmppfe rktvvlvgar gvgrrslknk lisydparfg tplphtsrpi retetdgkvy
      361 yfvsrevmea diadnkhlew gehsghlygt kldtlravir sgkmcvvdcs pqylkilntp
      421 efmpyvvfia apqlehfrqm heygrhhgyg ssrnltfdra msrhgsrrar tleslaslqe
      481 dedlkrtiee saqlqrayer yfdtilvnnn fektfeqlkd vidslstepq wvpiswvy
//
LOCUS       XP_077506403             131 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115775 [Amblyomma americanum].
ACCESSION   XP_077506403
VERSION     XP_077506403.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650277.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..131
                     /product="uncharacterized protein LOC144115775"
                     /calculated_mol_wt=14474
     CDS             1..131
                     /gene="LOC144115775"
                     /coded_by="XM_077650277.1:3604..3999"
                     /db_xref="GeneID:144115775"
ORIGIN      
        1 mchipgvkkk kvwrrgqeee ysrcgrwcta vsccrrclia rptdqawspp vdtpvlwimt
       61 ltlslergpc vnasgsttlp avvassqtss pvrgcpntdi qlrrrgttvs fhlillntpl
      121 veerrllkqg s
//
LOCUS       XP_077506404             414 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: protein PALS2-like [Amblyomma americanum].
ACCESSION   XP_077506404
VERSION     XP_077506404.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650278.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 2 indels
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..414
                     /product="LOW QUALITY PROTEIN: protein PALS2-like"
                     /calculated_mol_wt=47270
     Region          <2..54
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cl49608"
                     /db_xref="CDD:483948"
     Region          66..125
                     /region_name="SH3_MPP"
                     /note="Src Homology 3 domain of Membrane Protein,
                     Palmitoylated (or MAGUK p55 subfamily member) proteins;
                     cd11862"
                     /db_xref="CDD:212796"
     Site            order(68,92..93)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212796"
     Site            order(71,73,76,87,105..106,122,124..125)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212796"
     Region          187..401
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..414
                     /gene="LOC144115777"
                     /coded_by="XM_077650278.1:168..1412"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 1 base in 1 codon;
                     deleted 1 base in 1 codon"
                     /db_xref="GeneID:144115777"
ORIGIN      
        1 miarilaggi idrqgllhvg dtilevngvp vhtpeqlqlq lkkaqgsvaf kilpsyhdnl
       61 ptfqcfvkal ytyepskdtl lpckeiglpf kqgdilqvln qedpnwwqar rvsasgpasl
      121 ipsqeleerr qsfvrpeydy atktkmcgtk itkkkrktmy eskasaefek aelllyeeva
      181 rmppferktv vlvgargvgr rslknklisy dptrfgtplp htsrpirete tdgkvyyfvs
      241 revmeadiad nkylewgqhs ghlygtkldt lravihsgkm cvvdcspqyl kilntpefmp
      301 xvvciaapql ehfrqmheyg rhhgygssrn ltfdramsrh gsrrartles laslqededl
      361 krtieesaql qrayehyfdt ilvnnnfekt feqlkdvids lstepqwvpi swvy
//
LOCUS       XP_077506405             538 aa            linear   INV 25-NOV-2025
DEFINITION  protein PALS2-like [Amblyomma americanum].
ACCESSION   XP_077506405
VERSION     XP_077506405.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650279.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..538
                     /product="protein PALS2-like"
                     /calculated_mol_wt=61036
     Region          31..78
                     /region_name="L27"
                     /note="L27 domain; pfam02828"
                     /db_xref="CDD:460717"
     Region          101..178
                     /region_name="PDZ_MPP6-MPP2-like"
                     /note="PDZ domain of membrane palmitoylated protein 6
                     (MPP6), MPP2, and related domains; cd10832"
                     /db_xref="CDD:467268"
     Site            order(112..118,127,130,158,162,165..166)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467268"
     Region          190..249
                     /region_name="SH3_MPP"
                     /note="Src Homology 3 domain of Membrane Protein,
                     Palmitoylated (or MAGUK p55 subfamily member) proteins;
                     cd11862"
                     /db_xref="CDD:212796"
     Site            order(192,216..217)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212796"
     Site            order(195,197,200,211,229..230,246,248..249)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212796"
     Region          311..525
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..538
                     /gene="LOC144115780"
                     /coded_by="XM_077650279.1:342..1958"
                     /db_xref="GeneID:144115780"
ORIGIN      
        1 mespvmsclv qvqdhleekt ttfkpvstnn vellrdvcqa crrvsgrdaq elanilsgph
       61 fsallnahda vatksyepak plalekeapa mapetlndti rmvgirkndd eplgitvrve
      121 ddnlmiaril aggiidrqgl lhvgdtilev ngvpvhtpeq lqlqlkkaqg svafkilpsy
      181 hdnlptfqcf vkalytyeps kdtllpckei glpfkqgdil qvlnqedpnw wqarrvsasg
      241 paglipsqel eerrrafvrp eydyatktkm cgtkvtkkkr ktmyeskasa efekaelllf
      301 eevarmppfe rktvvlvgar gvgrrslknk lisydparfg tplphtsrpi retetdgkvy
      361 yfvsrevmea diadnkhlew gehsghlygt kldtlravir sgkmcvvdcs pqylkilntp
      421 efmpyvvfia apqlehfrqm heygrhhgyg ssrnltfdra msrhgsrrar tleslaslqe
      481 dedlkrtiee saqlqrayer yfdtilvnnn fektfeqlkd vidslstepq wvpiswvy
//
LOCUS       XP_077506406             133 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115781 [Amblyomma americanum].
ACCESSION   XP_077506406
VERSION     XP_077506406.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650280.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..133
                     /product="uncharacterized protein LOC144115781"
                     /calculated_mol_wt=14747
     CDS             1..133
                     /gene="LOC144115781"
                     /coded_by="XM_077650280.1:16..417"
                     /db_xref="GeneID:144115781"
ORIGIN      
        1 mchipgvkkk kkkvwrrgqe eeysrcgrwc tavsccrrcl iarptdqaws ppvdtpvlwi
       61 mtltlslerg pcvnasgstt lqavvassqt sspvrgcpnt diqlrrrgst vsfhlillnt
      121 plveerrllk qgs
//
LOCUS       XP_077506407             373 aa            linear   INV 25-NOV-2025
DEFINITION  protein PALS2-like [Amblyomma americanum].
ACCESSION   XP_077506407
VERSION     XP_077506407.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650281.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..373
                     /product="protein PALS2-like"
                     /calculated_mol_wt=42508
     Region          146..360
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..373
                     /gene="LOC144115785"
                     /coded_by="XM_077650281.1:47..1168"
                     /db_xref="GeneID:144115785"
ORIGIN      
        1 mseccptppe eaiafkwsap laaqapssqs ssvsrpfkhw qspsafsghg taaamqqrpm
       61 ffcflvqarr vsasgpagli psqeleerrr afvrpeydya tktkmcgtki tkkkrkamye
      121 skasaefeka elllyeevar mppferktvv lvgargvgrr slknklisyd parfgtplph
      181 tsrpiretet dgkvyyfvsr evmeadiadn kylewgehsg hlygtkldtl ravirsgkmc
      241 vvdcspqylk ilntpefmpy vvfiaapqle hfrqmheygc hhgygssrnl tfdramsrhg
      301 srrartlesl aslqededlk rtieesaqlq rayeryfdti lvnnnfektf eqlkdvidsl
      361 stepqwvpis wvy
//
LOCUS       XP_077506409             378 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115788 [Amblyomma americanum].
ACCESSION   XP_077506409
VERSION     XP_077506409.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650283.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..378
                     /product="uncharacterized protein LOC144115788"
                     /calculated_mol_wt=43144
     Region          5..225
                     /region_name="HAUS6_N"
                     /note="HAUS augmin-like complex subunit 6 N-terminus;
                     pfam14661"
                     /db_xref="CDD:464244"
     Region          153..>330
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     CDS             1..378
                     /gene="LOC144115788"
                     /coded_by="XM_077650283.1:36..1172"
                     /db_xref="GeneID:144115788"
ORIGIN      
        1 medvllvfld alhfkppddt kllqehfrgr qapatvrqvl hflyytydkk avesrmrniw
       61 pvktgesdkr freeitqwis tlqgdelatp yiqefsaall lrpgtpqcsk flaslsvfvl
      121 hyklkdsvhf sspfspaerp awklemvkka tiqtrsqnaa fkeeldafes ekakmqslle
      181 kkrsdlelsi khlqaqvqsf esddtlmsle klcrklraee rdskktleii neavetfsrs
      241 krvidlsslt dkqskflsss glvdvvgtfk dctelakkls aqfvkegctq qdleavklrv
      301 aalratlrrl qeqretakam kaeidqrlsa gckkpfvvln dkdidemcrp aaqpatepdv
      361 eefvnellav hrsqnrnq
//
LOCUS       XP_077506410             378 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115788 [Amblyomma americanum].
ACCESSION   XP_077506410
VERSION     XP_077506410.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650284.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..378
                     /product="uncharacterized protein LOC144115788"
                     /calculated_mol_wt=43144
     Region          5..225
                     /region_name="HAUS6_N"
                     /note="HAUS augmin-like complex subunit 6 N-terminus;
                     pfam14661"
                     /db_xref="CDD:464244"
     Region          153..>330
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     CDS             1..378
                     /gene="LOC144115788"
                     /coded_by="XM_077650284.1:133..1269"
                     /db_xref="GeneID:144115788"
ORIGIN      
        1 medvllvfld alhfkppddt kllqehfrgr qapatvrqvl hflyytydkk avesrmrniw
       61 pvktgesdkr freeitqwis tlqgdelatp yiqefsaall lrpgtpqcsk flaslsvfvl
      121 hyklkdsvhf sspfspaerp awklemvkka tiqtrsqnaa fkeeldafes ekakmqslle
      181 kkrsdlelsi khlqaqvqsf esddtlmsle klcrklraee rdskktleii neavetfsrs
      241 krvidlsslt dkqskflsss glvdvvgtfk dctelakkls aqfvkegctq qdleavklrv
      301 aalratlrrl qeqretakam kaeidqrlsa gckkpfvvln dkdidemcrp aaqpatepdv
      361 eefvnellav hrsqnrnq
//
LOCUS       XP_077506411             144 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115789 [Amblyomma americanum].
ACCESSION   XP_077506411
VERSION     XP_077506411.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650285.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..144
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..144
                     /product="uncharacterized protein LOC144115789"
                     /calculated_mol_wt=15595
     CDS             1..144
                     /gene="LOC144115789"
                     /coded_by="XM_077650285.1:85..519"
                     /db_xref="GeneID:144115789"
ORIGIN      
        1 mavadvsaam lvlidgvetr ayalldehgt hmenddgfly etegglrisv lrveepirnt
       61 ppptvpavve dgsrlapspl rqttppvvwq ptpspgpscy spppsghger dawndrksrf
      121 litkykkpri alaervalgn sprc
//
LOCUS       XP_077506412             350 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115793 [Amblyomma americanum].
ACCESSION   XP_077506412
VERSION     XP_077506412.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650286.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..350
                     /product="uncharacterized protein LOC144115793"
                     /calculated_mol_wt=38926
     Region          87..128
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cd00167"
                     /db_xref="CDD:238096"
     Site            order(88,118..119,121..122,124..125,127..128)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238096"
     CDS             1..350
                     /gene="LOC144115793"
                     /coded_by="XM_077650286.1:167..1219"
                     /db_xref="GeneID:144115793"
ORIGIN      
        1 mmeassdspl stpgtssaes ladetgmsps eppaenetpp sqppakkaka srgprrhllp
       61 rlrssrgrnl klpmhfkseg lkddyknwta kerkqlldal kehgsndlek laravptrsa
      121 tainyflmeh rckkdrivvd gatgrrtaqa lrilrraldv errrvdrsqa lvqvvasfqr
      181 qpfteptevd gaelpnfdni yqmlqdvmen kvpaalgpcd qfvvrrllgt lttlvatlnl
      241 neerevlkkq lawaatlpam ptrkdaegpr lepgekptlt fpsmsqrken lksswnplql
      301 paeviqkpwp ainklmaanv frserttsqg ksapstaaqg rsrrepqslq
//
LOCUS       XP_077506413             573 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115794 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506413
VERSION     XP_077506413.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650287.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..573
                     /product="uncharacterized protein LOC144115794 isoform X1"
                     /calculated_mol_wt=62163
     Region          <24..153
                     /region_name="Cym1"
                     /note="Zn-dependent peptidase, M16 (insulinase) family
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG1026"
                     /db_xref="CDD:440649"
     Region          230..>286
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          262..>398
                     /region_name="SF-CC1"
                     /note="splicing factor, CC1-like family; TIGR01622"
                     /db_xref="CDD:273721"
     Region          334..402
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cd00590"
                     /db_xref="CDD:409669"
     Region          476..544
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..573
                     /gene="LOC144115794"
                     /coded_by="XM_077650287.1:808..2529"
                     /db_xref="GeneID:144115794"
ORIGIN      
        1 masgilgvhv hgcytcllfc lffrekggay gvgvavrpag clsfysyrdp nmektldvfc
       61 dsvawltrge ftekdleeak lacfarvdkp itpgdcgaaa flhgitdemk qehrqrlfss
      121 tkddviaavk rcaseggcts svavigpenk ftetgrhwlv hrdgsnkpge dledgkeagq
      181 vaeesaahaq kkdssskgds sssssgsdsd tsdsdrdssa drfllkihdl penitaeqva
      241 rhlkqagvrv sggrlgilll trskgdlpig rafvevaske dqeralsyaw esaklsghri
      301 kafrgqvddv rryvmsrrpa lsalhkdvts scvvqvrnlp vgateeklke lfshckvtqv
      361 erekfssgra qraafmrfmc pdeaqralav ngrklmgqkl elclvpedya drllegdlln
      421 lasyaeqkgs rigtrrvsgt arhaakeasl regatnfrla epheleailq tseglhrvva
      481 eglpfsiaqi enflwpakps lvvrvrrasg vmsgkvvitf asladaqeta kknghmifgr
      541 svrikllmpg tpsgeendsv tadvatassp sss
//
LOCUS       XP_077506414             465 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115794 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506414
VERSION     XP_077506414.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650288.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..465
                     /product="uncharacterized protein LOC144115794 isoform X2"
                     /calculated_mol_wt=50431
     Region          122..>178
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          226..294
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cd00590"
                     /db_xref="CDD:409669"
     Region          368..436
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..465
                     /gene="LOC144115794"
                     /coded_by="XM_077650288.1:222..1619"
                     /db_xref="GeneID:144115794"
ORIGIN      
        1 mkqehrqrlf sstkddviaa vkrcaseggc tssvavigpe nkftetgrhw lvhrdgsnkp
       61 gedledgkea gqvaeesaah aqkkdssskg dssssssgsd sdtsdsdrds sadrfllkih
      121 dlpenitaeq varhlkqagv rvsggrlgil lltrskgdlp igrafvevas kedqeralsy
      181 awesaklsgh rikafrgqvd dvrryvmsrr palsalhkdv tsscvvqvrn lpvgateekl
      241 kelfshckvt qverekfssg raqraafmrf mcpdeaqral avngrklmgq klelclvped
      301 yadrllegdl lnlasyaeqk gsrigtrrvs gtarhaakea slregatnfr laepheleai
      361 lqtseglhrv vaeglpfsia qienflwpak pslvvrvrra sgvmsgkvvi tfasladaqe
      421 takknghmif grsvrikllm pgtpsgeend svtadvatas spsss
//
LOCUS       XP_077506415             430 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115795 [Amblyomma americanum].
ACCESSION   XP_077506415
VERSION     XP_077506415.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650289.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..430
                     /product="uncharacterized protein LOC144115795"
                     /calculated_mol_wt=48975
     Region          165..>224
                     /region_name="Lebercilin"
                     /note="Ciliary protein causing Leber congenital amaurosis
                     disease; pfam15619"
                     /db_xref="CDD:464776"
     CDS             1..430
                     /gene="LOC144115795"
                     /coded_by="XM_077650289.1:24..1316"
                     /db_xref="GeneID:144115795"
ORIGIN      
        1 mrgyhhhqya ttfvshprpa flppdrpsrs arfccqryvg avvrsssfgv vrviamfayv
       61 rfrdddmktv lpisrfrsfq pkglkdfatn kwyevfwedn eqsgyyqaqi vrlfdtkeea
      121 erqkripvpp rpsdteteet tpssddylre vmrkaaaekk kknnsqrilr ekqlqqlmen
      181 epdeqahele qlrrenkdlr erlktmekal cskifdaeql msrhcsckqk rpeirtserv
      241 pakgavermp alsappgpek vppaapeakp adpparesqr pvavlappqp slghnpatpe
      301 ecaadqlegl pfqatgskvf lghgvsltre gfnhlmsrqk dsvfvreacv qifstaglvg
      361 ksitgnmcnr tkadpkppld qqkyaalsaf fqhylearye adearrrhrs lnkivagkla
      421 dvmkmqrrdv
//
LOCUS       XP_077506416             908 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115796 [Amblyomma americanum].
ACCESSION   XP_077506416
VERSION     XP_077506416.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650290.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..908
                     /product="uncharacterized protein LOC144115796"
                     /calculated_mol_wt=102477
     CDS             1..908
                     /gene="LOC144115796"
                     /coded_by="XM_077650290.1:44..2770"
                     /db_xref="GeneID:144115796"
ORIGIN      
        1 miaarkvmde mqstrrakkr krylepgeey avprttllyq krrdeerqpp atvpnifpkt
       61 giqnhsppta npvidqqsse iatpyasvsd lreqqncred hevhinddcy rkpdeesiya
      121 dscsanednv hscsageddf sddrcrnadq ensdschsad eddvadhcnr nadednthsc
      181 rsaddgdsdr efsdffsier lpnsdvtvrd amfilmayts ssglnwvdme klvgvinlfl
      241 gkpilptsqy llrkvwkrwe admvkrhyfc eecgaiipnp ekreflcpsc ntsnsaqnff
      301 atvsiekqle vmltndtvaq tlltslkkkr atasdpgpse nkvirditdg klyqrqmgka
      361 awsdvsltfn tdgarafkcs ksslwpiqav vnelpvplrw khvllcglyf gkshpnmasf
      421 levftetvrr avnwecdgek yssnmkftcc cvdaparaav lnikqfngyy gcsychhkgv
      481 lvnrsvkypv addgghpepr tnrsvrsdmk sslisgepsr gvkgfsplar lpdfdlvwgv
      541 cpdymhcvle gvtrqladtw fgcvqsasyi gvpatlsqvn trllsikppq wfsrlprsig
      601 eralwkasew kwwllfysvp clegilpeey lhhfcslvaa vyillkdemt kediheamdk
      661 lscfvlsmee lygreamtfn vhqllhlpks vlelgplwsh safvfesgng mllklisdan
      721 gvplqilerf amrlqitkla ntlnptsqvq avydtlmpss cagksstekp lgrgtllnem
      781 deqvrcavqe klgqvlplmk fkrmdvhgqr lhvegyrrak ktrncifrcv qgkyyilesv
      841 fqlpdstcll lctclasesy rdlphihkca ktskkvvlaa dkvssvsvam dvgrvcyvgd
      901 mpnlfekd
//
LOCUS       XP_077506417             350 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115799 [Amblyomma americanum].
ACCESSION   XP_077506417
VERSION     XP_077506417.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650291.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..350
                     /product="uncharacterized protein LOC144115799"
                     /calculated_mol_wt=38899
     Region          87..128
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cd00167"
                     /db_xref="CDD:238096"
     Site            order(88,118..119,121..122,124..125,127..128)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238096"
     CDS             1..350
                     /gene="LOC144115799"
                     /coded_by="XM_077650291.1:184..1236"
                     /db_xref="GeneID:144115799"
ORIGIN      
        1 mmeassdspl stpgtssaes ladetgmsps eppaenetpp sqppakkaka srgprrhllp
       61 rlrssrgrnl klpmhfkseg lkddyknwta kerkqlldal kehgssdlek laravptrsa
      121 tainyflmeh rckkdrivvd gatgrrtaqa lrilrraldv errrvdrsqa lvqvvasfqr
      181 qpfteptevd gaelpnfdni yqmlqdvmen kvpaalgpcd qfvvrrllgt lttlvatlnl
      241 neerevlkkq lawaatlpam ptrkdaegpr lepgekptlt fpsmsqrken lksswnplql
      301 paeviqkpwp ainklmaanv frserttsqg ksapstaaqg rsrrepqslq
//
LOCUS       XP_077506418             840 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115800 [Amblyomma americanum].
ACCESSION   XP_077506418
VERSION     XP_077506418.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650292.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..840
                     /product="uncharacterized protein LOC144115800"
                     /calculated_mol_wt=92197
     Region          60..>271
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..840
                     /gene="LOC144115800"
                     /coded_by="XM_077650292.1:944..3466"
                     /db_xref="GeneID:144115800"
ORIGIN      
        1 mpllrlsaar alrendslqf nsccpfcifv nssaacgfvc epamerrtgq kyrtkyaygk
       61 rkrkppipkq krpaatephe rrsalissha dvsseaslpl esatssesgg lapsashdia
      121 vqyrpghssv faaasggavp vqrddgevfp kqkhtvqvhl psesitgses vgpapstsrn
      181 iavqyrpghs svfaaasaga vpvqrddgev fpkqkrivqv hlpsesmtgs esgdpapsts
      241 rdiavqhrpg qssvfaaasd dampaerdds elfpkqkrtv qvhleprfvt leaaeeqacs
      301 vratlravss terkfgfarq teeesapded eftliqasvm nsiigsllcp kcskkrlsvk
      361 hetklglavk lvlscgacgp iashwsskrk segrtfevni ramqaiktig kgptalndfw
      421 atmnvshrgl hqktyqghlk klfkpgaeea aqnifadavl avkdvyskmq psspsnitvv
      481 ydgtwltrdh ssrigvgcii efytglvldc tvlsnfclgc cqqpaesdps ygtwieqhqc
      541 qkntdaasgq meveaalilf rrsfssyglr ytnviwdgds rtylalckde vygfipltke
      601 dcinhvqkrm gtalrtlvar akkgeplggk ggltqdlikk ltnyyglalr rntevsdmqr
      661 avmatlyhvt stddephhel cppgpdswcr hrsaqakmep ppphkyklpr rvaeallpvy
      721 qrlsdpqlle rckgnktqna aeslhsvvws miskdqhasl favetavhea varynfgnlr
      781 aytevcksvg ikpgslafer aqekdkqrkr kacsaektke qrhkktpask dtkyyspgaf
//
LOCUS       XP_077506419             573 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115801 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506419
VERSION     XP_077506419.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650293.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..573
                     /product="uncharacterized protein LOC144115801 isoform X1"
                     /calculated_mol_wt=62166
     Region          <24..153
                     /region_name="Cym1"
                     /note="Zn-dependent peptidase, M16 (insulinase) family
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG1026"
                     /db_xref="CDD:440649"
     Region          <225..>400
                     /region_name="PABP-1234"
                     /note="polyadenylate binding protein, human types 1, 2, 3,
                     4 family; TIGR01628"
                     /db_xref="CDD:130689"
     Region          334..402
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cd00590"
                     /db_xref="CDD:409669"
     Region          476..544
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..573
                     /gene="LOC144115801"
                     /coded_by="XM_077650293.1:283..2004"
                     /db_xref="GeneID:144115801"
ORIGIN      
        1 masgilgvhv hgcytcllfc lffrekggay gvgvavrpag clsfysyrdp nmektldvfc
       61 dsvawltrge fteedleeak lacfarvdkp itpgdcgaaa flhgitdemk qehrqrlfss
      121 tkddviaavk rcaseggcts svavigpenk ftetgrhwlv hrdgsnkpge dledgkeagq
      181 vaeesaahaq kkdssskgds sssssgsdsd tsdsdrdssa drfllkihdl penitaeqva
      241 rhlkqagvrv sggrlgilll trskgdlpig rafvevaske dqeralsyaw esaklsghri
      301 kafrgqvddv rryvmsrrpa lsalhkgvts scvvqvrnlp vgateeklke lfshckvtqv
      361 erekfnsgra qraafmrfmc pdeaqralav ngrklmgqkl elclvpedya drllegdlln
      421 lasyaeqkgs rigtrrvsgt arraakeasl regatnfrla epheleailq tsegqhrvva
      481 eglpfsiaqi enflwpakps lvvrvrrasg vmsgkvvitf asladaqeta kknghmifgr
      541 svrikllmpg tpsgeendsv tadvatassp sss
//
LOCUS       XP_077506420             496 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115801 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506420
VERSION     XP_077506420.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650294.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..496
                     /product="uncharacterized protein LOC144115801 isoform X2"
                     /calculated_mol_wt=53751
     Region          <148..>323
                     /region_name="PABP-1234"
                     /note="polyadenylate binding protein, human types 1, 2, 3,
                     4 family; TIGR01628"
                     /db_xref="CDD:130689"
     Region          257..325
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cd00590"
                     /db_xref="CDD:409669"
     Region          399..467
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..496
                     /gene="LOC144115801"
                     /coded_by="XM_077650294.1:241..1731"
                     /db_xref="GeneID:144115801"
ORIGIN      
        1 mhaklkknrv dkpitpgdcg aaaflhgitd emkqehrqrl fsstkddvia avkrcasegg
       61 ctssvavigp enkftetgrh wlvhrdgsnk pgedledgke agqvaeesaa haqkkdsssk
      121 gdssssssgs dsdtsdsdrd ssadrfllki hdlpenitae qvarhlkqag vrvsggrlgi
      181 llltrskgdl pigrafveva skedqerals yawesaklsg hrikafrgqv ddvrryvmsr
      241 rpalsalhkg vtsscvvqvr nlpvgateek lkelfshckv tqverekfns graqraafmr
      301 fmcpdeaqra lavngrklmg qklelclvpe dyadrllegd llnlasyaeq kgsrigtrrv
      361 sgtarraake aslregatnf rlaephelea ilqtsegqhr vvaeglpfsi aqienflwpa
      421 kpslvvrvrr asgvmsgkvv itfasladaq etakknghmi fgrsvrikll mpgtpsgeen
      481 dsvtadvata sspsss
//
LOCUS       XP_077506421             465 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115801 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077506421
VERSION     XP_077506421.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650295.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..465
                     /product="uncharacterized protein LOC144115801 isoform X3"
                     /calculated_mol_wt=50434
     Region          <117..>292
                     /region_name="PABP-1234"
                     /note="polyadenylate binding protein, human types 1, 2, 3,
                     4 family; TIGR01628"
                     /db_xref="CDD:130689"
     Region          226..294
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cd00590"
                     /db_xref="CDD:409669"
     Region          368..436
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..465
                     /gene="LOC144115801"
                     /coded_by="XM_077650295.1:294..1691"
                     /db_xref="GeneID:144115801"
ORIGIN      
        1 mkqehrqrlf sstkddviaa vkrcaseggc tssvavigpe nkftetgrhw lvhrdgsnkp
       61 gedledgkea gqvaeesaah aqkkdssskg dssssssgsd sdtsdsdrds sadrfllkih
      121 dlpenitaeq varhlkqagv rvsggrlgil lltrskgdlp igrafvevas kedqeralsy
      181 awesaklsgh rikafrgqvd dvrryvmsrr palsalhkgv tsscvvqvrn lpvgateekl
      241 kelfshckvt qverekfnsg raqraafmrf mcpdeaqral avngrklmgq klelclvped
      301 yadrllegdl lnlasyaeqk gsrigtrrvs gtarraakea slregatnfr laepheleai
      361 lqtsegqhrv vaeglpfsia qienflwpak pslvvrvrra sgvmsgkvvi tfasladaqe
      421 takknghmif grsvrikllm pgtpsgeend svtadvatas spsss
//
LOCUS       XP_077506422             374 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115805 [Amblyomma americanum].
ACCESSION   XP_077506422
VERSION     XP_077506422.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650296.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..374
                     /product="uncharacterized protein LOC144115805"
                     /calculated_mol_wt=41275
     Region          <114..>161
                     /region_name="Abhydrolase"
                     /note="alpha/beta hydrolases; cl21494"
                     /db_xref="CDD:473884"
     CDS             1..374
                     /gene="LOC144115805"
                     /coded_by="XM_077650296.1:78..1202"
                     /db_xref="GeneID:144115805"
ORIGIN      
        1 mlraacglsc sarykgtafi sldtlelskm atwkqyngyl hksistpqlg lalrlalllf
       61 lggkktqllg laqrlalllf lggkkpqllg laqrlalllf lggkkpqlqe svalasrtdl
      121 vvvapkyrln msefftanss eepgmllqrq aldwlracis sccdqtgsta lqeydsgass
      181 hgshllnras lsvleglnac ldyraevfvp elnrsfmvgr srffgcqnph qkggamrglp
      241 hsflnrfvql gleplkrddl ltlvrylhpa lpedtlscmv lfnekglpmf vkgccmaavq
      301 rpgerrqrrs mlettaaggr isqchlprpe apkgqqlsav stavdfgkdf fqgvfwqphc
      361 dfprfrslft efsp
//
LOCUS       XP_077506423            1720 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: cadherin 86C [Amblyomma americanum].
ACCESSION   XP_077506423
VERSION     XP_077506423.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650297.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1720
                     /product="LOW QUALITY PROTEIN: cadherin 86C"
                     /calculated_mol_wt=185829
     Region          199..286
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          311..410
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          424..536
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(431..432,496,498,532,534..535)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          545..641
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(551..552,606,608,637,639..640)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          665..744
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     CDS             1..1720
                     /gene="Cad86C"
                     /coded_by="XM_077650297.1:102..5264"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:144115807"
ORIGIN      
        1 mglapagaag dsvlrltpwr wearrkrttr saraesayga ggfeswstle escssqrdrr
       61 rsrhrrctaa mwlcmlllld ignvegnapn idyshnmrvl klpketpigt ivyrlkgsda
      121 dsdvltfgaq davgkalfqf rsvqfsqadv ilkkalteee ytiqvfvtdg vettevesti
      181 ivtngtglph pfvdystllr vpentkvnss igyvtarere nsnlpvtfel rgsdkfkiry
      241 efgprgtska viqlielldy etrkvyeldv lalnawtder ydtrnvamls lavvaqdvpd
      301 tpprfhnvpp vvtvsdsapp gtsvlnltae dgdygnprnl tyrldkenpw sshftvnpqt
      361 gevkttrpis eladkyggta vyilkvaare ssgtaeypaq eastqlavvl msrenhppaf
      421 asevyagfva ehsapstavq wapgvmakvy drdqgrnstf rlylegeaas vfsihpqsas
      481 readfailvq dsskldyeva npkyvefmiv aaeteavqpl tssvavrvni adtndhapmf
      541 tsdmyevvva edakpgtvia avtatdpdtg sfgtikytsl ngpiarnlkl dadtgqvtll
      601 ssegldresv peyaltveaq dgggrrastq llvrlgdand napsfrqpry davltadlad
      661 faeplivqvp dadapgpnsn vtyeivsgny eqkfrinpvs galslsapls aqrgaraglp
      721 lvltvrahdq gipvrsasvp vrvhtqeyln rsivvvlpgs edqvrprken ierglsdllg
      781 anvniysimp hngsknatva rawavyesqp idlvsfgkim snlygknyeq isveretlll
      841 krsgndrvfw illivlilii lllllimlcc cwkyflrnqr prssskvsps asagaattdq
      901 svrerrsttd kastavngsv psgrrgwqgd ggsprpadgs vptqsvavgt tagadeaarq
      961 ggtvenpprg rpasrerlvl hgssldtdav vhageggghr lgdflvvrrd aaaarrrrrd
     1021 egpdsdaepy rrapsspvsd tfgttrsvey sggslggvgg prrteilyir spppdvasva
     1081 gdyqedsssr rwrrlreeea ddsgnirhra aanrrenarr vrfsryhkvd gasfvlqshs
     1141 pdgvedatss tsglprdqvw ivpqeprgaa qrdavryeep agrpipaarn arlramhrri
     1201 gvdseelgts dfgesfeece lrskniland lpqvhggead gpsfsrvaes sfqdrsrgqq
     1261 gtatsgmagv ppqvfqvdat tavqqqirts qiedrsvqag esvqassvgs haalvpvqst
     1321 dlteqtsslm vpqrkaavss gtstqtttlt trtqqqqsql dssvaspsla tnisgpsise
     1381 apvsfthsdg saarfsdtvp tdpanvstvs hvaqvhhiqq egaltqshiq stlqslglqg
     1441 pqlvaitgms pvffstqlpg tqvisqpldi ahpsyprttv hhqpsgsapt hysteqtthf
     1501 lhprdqvpha pshlseskqd lisdrheqag tpslttegra gtlsqdrish snlvekvsat
     1561 aikenqegsa vrgpttgpvp satvhdesnk arfhvplsvr asghtteaee tpthqrystl
     1621 kittkvittd gsilhegsat qtgaasdsel eaierelkts lgkldesgqd dsdsgigaga
     1681 knlrlkknaf iekkslftia ydgvnterir tclgssspsp
//
LOCUS       XP_077506424             368 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylcholine:ceramide cholinephosphotransferase 2-like
            [Amblyomma americanum].
ACCESSION   XP_077506424
VERSION     XP_077506424.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650298.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..368
                     /product="phosphatidylcholine:ceramide
                     cholinephosphotransferase 2-like"
                     /calculated_mol_wt=41460
     Region          239..311
                     /region_name="PAP2_C"
                     /note="PAP2 superfamily C-terminal; pfam14360"
                     /db_xref="CDD:464150"
     CDS             1..368
                     /gene="LOC144115808"
                     /coded_by="XM_077650298.1:898..2004"
                     /db_xref="GeneID:144115808"
ORIGIN      
        1 miqvmdttls atgaegqpll cngaasgass gvggtsglhh swpdhhasca gcdeyrnrrd
       61 kngdllacdt visigaaasp verggeprll qekwktlvaf tflsanliln ltclayvher
      121 ipdrakfppl pdvffdlfpp ldwaldvses iiifciwstl glllihryrw ivlqrvffim
      181 gllyfmrsit mfvtqvpvas ttyycspkan stnpllimkr vaqllsgfgl singqhtfcg
      241 dyiysghtvi ltlaylvvre yspqrckylh lvylvlsavg ilmvllsrgh ytvdvvigyy
      301 vtsrvfwiyh tlannmslkv asqnnylsrs wwfplfsyfe knvggvvprq yewplpwprr
      361 wlprtris
//
LOCUS       XP_077506425             368 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylcholine:ceramide cholinephosphotransferase 2-like
            [Amblyomma americanum].
ACCESSION   XP_077506425
VERSION     XP_077506425.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650299.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..368
                     /product="phosphatidylcholine:ceramide
                     cholinephosphotransferase 2-like"
                     /calculated_mol_wt=41460
     Region          239..311
                     /region_name="PAP2_C"
                     /note="PAP2 superfamily C-terminal; pfam14360"
                     /db_xref="CDD:464150"
     CDS             1..368
                     /gene="LOC144115808"
                     /coded_by="XM_077650299.1:324..1430"
                     /db_xref="GeneID:144115808"
ORIGIN      
        1 miqvmdttls atgaegqpll cngaasgass gvggtsglhh swpdhhasca gcdeyrnrrd
       61 kngdllacdt visigaaasp verggeprll qekwktlvaf tflsanliln ltclayvher
      121 ipdrakfppl pdvffdlfpp ldwaldvses iiifciwstl glllihryrw ivlqrvffim
      181 gllyfmrsit mfvtqvpvas ttyycspkan stnpllimkr vaqllsgfgl singqhtfcg
      241 dyiysghtvi ltlaylvvre yspqrckylh lvylvlsavg ilmvllsrgh ytvdvvigyy
      301 vtsrvfwiyh tlannmslkv asqnnylsrs wwfplfsyfe knvggvvprq yewplpwprr
      361 wlprtris
//
LOCUS       XP_077506426             368 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylcholine:ceramide cholinephosphotransferase 2-like
            [Amblyomma americanum].
ACCESSION   XP_077506426
VERSION     XP_077506426.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650300.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..368
                     /product="phosphatidylcholine:ceramide
                     cholinephosphotransferase 2-like"
                     /calculated_mol_wt=41460
     Region          239..311
                     /region_name="PAP2_C"
                     /note="PAP2 superfamily C-terminal; pfam14360"
                     /db_xref="CDD:464150"
     CDS             1..368
                     /gene="LOC144115808"
                     /coded_by="XM_077650300.1:350..1456"
                     /db_xref="GeneID:144115808"
ORIGIN      
        1 miqvmdttls atgaegqpll cngaasgass gvggtsglhh swpdhhasca gcdeyrnrrd
       61 kngdllacdt visigaaasp verggeprll qekwktlvaf tflsanliln ltclayvher
      121 ipdrakfppl pdvffdlfpp ldwaldvses iiifciwstl glllihryrw ivlqrvffim
      181 gllyfmrsit mfvtqvpvas ttyycspkan stnpllimkr vaqllsgfgl singqhtfcg
      241 dyiysghtvi ltlaylvvre yspqrckylh lvylvlsavg ilmvllsrgh ytvdvvigyy
      301 vtsrvfwiyh tlannmslkv asqnnylsrs wwfplfsyfe knvggvvprq yewplpwprr
      361 wlprtris
//
LOCUS       XP_077506427             368 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylcholine:ceramide cholinephosphotransferase 2-like
            [Amblyomma americanum].
ACCESSION   XP_077506427
VERSION     XP_077506427.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650301.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..368
                     /product="phosphatidylcholine:ceramide
                     cholinephosphotransferase 2-like"
                     /calculated_mol_wt=41460
     Region          239..311
                     /region_name="PAP2_C"
                     /note="PAP2 superfamily C-terminal; pfam14360"
                     /db_xref="CDD:464150"
     CDS             1..368
                     /gene="LOC144115808"
                     /coded_by="XM_077650301.1:654..1760"
                     /db_xref="GeneID:144115808"
ORIGIN      
        1 miqvmdttls atgaegqpll cngaasgass gvggtsglhh swpdhhasca gcdeyrnrrd
       61 kngdllacdt visigaaasp verggeprll qekwktlvaf tflsanliln ltclayvher
      121 ipdrakfppl pdvffdlfpp ldwaldvses iiifciwstl glllihryrw ivlqrvffim
      181 gllyfmrsit mfvtqvpvas ttyycspkan stnpllimkr vaqllsgfgl singqhtfcg
      241 dyiysghtvi ltlaylvvre yspqrckylh lvylvlsavg ilmvllsrgh ytvdvvigyy
      301 vtsrvfwiyh tlannmslkv asqnnylsrs wwfplfsyfe knvggvvprq yewplpwprr
      361 wlprtris
//
LOCUS       XP_077506428             368 aa            linear   INV 25-NOV-2025
DEFINITION  phosphatidylcholine:ceramide cholinephosphotransferase 2-like
            [Amblyomma americanum].
ACCESSION   XP_077506428
VERSION     XP_077506428.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650302.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..368
                     /product="phosphatidylcholine:ceramide
                     cholinephosphotransferase 2-like"
                     /calculated_mol_wt=41460
     Region          239..311
                     /region_name="PAP2_C"
                     /note="PAP2 superfamily C-terminal; pfam14360"
                     /db_xref="CDD:464150"
     CDS             1..368
                     /gene="LOC144115808"
                     /coded_by="XM_077650302.1:223..1329"
                     /db_xref="GeneID:144115808"
ORIGIN      
        1 miqvmdttls atgaegqpll cngaasgass gvggtsglhh swpdhhasca gcdeyrnrrd
       61 kngdllacdt visigaaasp verggeprll qekwktlvaf tflsanliln ltclayvher
      121 ipdrakfppl pdvffdlfpp ldwaldvses iiifciwstl glllihryrw ivlqrvffim
      181 gllyfmrsit mfvtqvpvas ttyycspkan stnpllimkr vaqllsgfgl singqhtfcg
      241 dyiysghtvi ltlaylvvre yspqrckylh lvylvlsavg ilmvllsrgh ytvdvvigyy
      301 vtsrvfwiyh tlannmslkv asqnnylsrs wwfplfsyfe knvggvvprq yewplpwprr
      361 wlprtris
//
LOCUS       XP_077506429             141 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115809 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506429
VERSION     XP_077506429.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650303.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..141
                     /product="uncharacterized protein LOC144115809 isoform X1"
                     /calculated_mol_wt=14596
     CDS             1..141
                     /gene="LOC144115809"
                     /coded_by="XM_077650303.1:311..736"
                     /db_xref="GeneID:144115809"
ORIGIN      
        1 msplvvrira davplqqepl sqqpgdgtav aataqhgaqq altqshtspa splqqapmdv
       61 aesqetlagp sqqapmdvte sqetlpgpsq qasmdvatml diqpgpsqaf qltpvsfmss
      121 cvvsiqvfsl hevgcrcnsa m
//
LOCUS       XP_077506430             140 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115809 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506430
VERSION     XP_077506430.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650304.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..140
                     /product="uncharacterized protein LOC144115809 isoform X2"
                     /calculated_mol_wt=14468
     CDS             1..140
                     /gene="LOC144115809"
                     /coded_by="XM_077650304.1:314..736"
                     /db_xref="GeneID:144115809"
ORIGIN      
        1 msplvvrira davplqepls qqpgdgtava ataqhgaqqa ltqshtspas plqqapmdva
       61 esqetlagps qqapmdvtes qetlpgpsqq asmdvatmld iqpgpsqafq ltpvsfmssc
      121 vvsiqvfslh evgcrcnsam
//
LOCUS       XP_077506431            1394 aa            linear   INV 25-NOV-2025
DEFINITION  PDZ domain-containing guanine nucleotide exchange factor isoform X1
            [Amblyomma americanum].
ACCESSION   XP_077506431
VERSION     XP_077506431.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650305.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1394
                     /product="PDZ domain-containing guanine nucleotide
                     exchange factor isoform X1"
                     /calculated_mol_wt=152379
     Region          135..245
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(200..201,210..212)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Site            order(233..235,239..241)
                     /site_type="other"
                     /note="flexible hinge region"
                     /db_xref="CDD:237999"
     Region          266..375
                     /region_name="RasGEFN"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     GTPases; N-terminal motif; smart00229"
                     /db_xref="CDD:214571"
     Site            order(285,325..326,329,332..333,368..369,372)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:100121"
     Region          382..465
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cl49608"
                     /db_xref="CDD:483948"
     Site            order(394..400,412,415,445,449,452..453)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467153"
     Region          675..757
                     /region_name="RA_PDZ-GEF1"
                     /note="Ras-associating (RA) domain found in PDZ
                     domain-containing guanine nucleotide exchange factor 1
                     (PDZ-GEF1) and similar proteins; cd01785"
                     /db_xref="CDD:340483"
     Region          780..984
                     /region_name="RasGEF"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     small GTPases. Small GTP-binding proteins of the Ras
                     superfamily function as molecular switches in fundamental
                     events such as signal transduction, cytoskeleton dynamics
                     and intracellular trafficking; cd00155"
                     /db_xref="CDD:238087"
     Site            order(813,824..825,827..829,831..832,835..836,839,872,
                     875..876,878..880,882..885,887..888,908,911,916..918,921,
                     936..938,941..943,945..947,949..952,966,970)
                     /site_type="other"
                     /note="Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:238087"
     CDS             1..1394
                     /gene="PDZ-GEF"
                     /coded_by="XM_077650305.1:360..4544"
                     /db_xref="GeneID:144115811"
ORIGIN      
        1 mnlerllale pdevavetrh phhhlqqlpl dcqqtalhke gllksnrssr ssdtssaysg
       61 sdtmqsvqss lednevdlsg lvestvdsde eedlaesids ltvrdayhec lekdpndrtd
      121 ddietllefm qplpafanmt lsvrrelcav mvfavvekag tvvmndgeel dswsvivngq
      181 vevempdgts htlsmgdsfg itptmekmhh vgtmrtrvdd cqfvciaqgd ycrilhrsee
      241 ntrkleedgr vvlvtehrtv dagsreghiv irgtpdrlla qlfeenaldp gyiedfllty
      301 rtfipsslvv cnkllswfnd pqlrdkvtrv vllwvnnhft dfetdpemme fleqfeqcle
      361 ankmhaqlrl lniacatkar trvvtlarss rddelpfqil ggyetgnaif vakvdkansk
      421 sedgwlkrgd eilevngqsf atmsynqame ilrkathlci tvrsnllefk emlnnpekam
      481 rprgrkssei lrlqpdprlr lsshlewsdv vglplgvggl plgvgwsgqh pssaagspap
      541 atgasvsssg sctgngdvac kedkkgfmtl ghraklkkal akmnfipktl naesqlnssd
      601 dslysthsgs svgsscgnhr vgrsgsspah aiapggcggg ggqlyhshsn pdlsslivtp
      661 hcegvdapgr dfpehvlkvy kadqtckyll vhrettarev vmlalrefgi tdssslyslc
      721 evsvgeggli kqrrlpeqmq nlaekiglss ryylrnnast dplvpddmas ellresqvsf
      781 lqlnsvevat qltledfsif rqiepteyid dlfevksayg apmlamfael vnremfwvvs
      841 eicgetnpir rmktvkqfvk varqckeckn fnsmfailsg lghgavsrlr stwdklptky
      901 qkmfedlqel mdpsrnmcky rnlinseqtr ppiipfypiv kkdlsfihfg ndtivdnlvn
      961 feklrmiake vrqlmkmcsv pydlysmlea ggsplnpala slnsfaaaav aggagaalar
     1021 rrkrstaqps pkkmfeeaqm vrrvkaylsh lkvvadegrl lamsfeceph papsaipvvl
     1081 psnahpqrkr hpsprkrhps ptlstassns stsegkksin saggvkfgtd spqamrklla
     1141 lsdqskvrph qpprppasps pgvmrrgsat vrdapaavgg asanchgrsh sdagyasvqp
     1201 vdlsaesssv tslnnlplrk shasacsvts adsgvgsyqe sdsgrcstgy gspqqrrisg
     1261 tgppsppivm rnrksmppft hgvpvlpplv pgqhqgpmsp scllmppppp rdpppvpkga
     1321 gpprrppdyn vaaqmaarsr qaqrlhrars qevmaataaa vaaassface vavdpatdhe
     1381 adeededeaq vsav
//
LOCUS       XP_077506432            1359 aa            linear   INV 25-NOV-2025
DEFINITION  PDZ domain-containing guanine nucleotide exchange factor isoform X2
            [Amblyomma americanum].
ACCESSION   XP_077506432
VERSION     XP_077506432.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650306.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1359
                     /product="PDZ domain-containing guanine nucleotide
                     exchange factor isoform X2"
                     /calculated_mol_wt=148813
     Region          135..245
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(200..201,210..212)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Site            order(233..235,239..241)
                     /site_type="other"
                     /note="flexible hinge region"
                     /db_xref="CDD:237999"
     Region          266..375
                     /region_name="RasGEFN"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     GTPases; N-terminal motif; smart00229"
                     /db_xref="CDD:214571"
     Site            order(285,325..326,329,332..333,368..369,372)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:100121"
     Region          382..465
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cl49608"
                     /db_xref="CDD:483948"
     Site            order(394..400,412,415,445,449,452..453)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467153"
     Region          675..757
                     /region_name="RA_PDZ-GEF1"
                     /note="Ras-associating (RA) domain found in PDZ
                     domain-containing guanine nucleotide exchange factor 1
                     (PDZ-GEF1) and similar proteins; cd01785"
                     /db_xref="CDD:340483"
     Region          780..984
                     /region_name="RasGEF"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     small GTPases. Small GTP-binding proteins of the Ras
                     superfamily function as molecular switches in fundamental
                     events such as signal transduction, cytoskeleton dynamics
                     and intracellular trafficking; cd00155"
                     /db_xref="CDD:238087"
     Site            order(813,824..825,827..829,831..832,835..836,839,872,
                     875..876,878..880,882..885,887..888,908,911,916..918,921,
                     936..938,941..943,945..947,949..952,966,970)
                     /site_type="other"
                     /note="Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:238087"
     CDS             1..1359
                     /gene="PDZ-GEF"
                     /coded_by="XM_077650306.1:305..4384"
                     /db_xref="GeneID:144115811"
ORIGIN      
        1 mnlerllale pdevavetrh phhhlqqlpl dcqqtalhke gllksnrssr ssdtssaysg
       61 sdtmqsvqss lednevdlsg lvestvdsde eedlaesids ltvrdayhec lekdpndrtd
      121 ddietllefm qplpafanmt lsvrrelcav mvfavvekag tvvmndgeel dswsvivngq
      181 vevempdgts htlsmgdsfg itptmekmhh vgtmrtrvdd cqfvciaqgd ycrilhrsee
      241 ntrkleedgr vvlvtehrtv dagsreghiv irgtpdrlla qlfeenaldp gyiedfllty
      301 rtfipsslvv cnkllswfnd pqlrdkvtrv vllwvnnhft dfetdpemme fleqfeqcle
      361 ankmhaqlrl lniacatkar trvvtlarss rddelpfqil ggyetgnaif vakvdkansk
      421 sedgwlkrgd eilevngqsf atmsynqame ilrkathlci tvrsnllefk emlnnpekam
      481 rprgrkssei lrlqpdprlr lsshlewsdv vglplgvggl plgvgwsgqh pssaagspap
      541 atgasvsssg sctgngdvac kedkkgfmtl ghraklkkal akmnfipktl naesqlnssd
      601 dslysthsgs svgsscgnhr vgrsgsspah aiapggcggg ggqlyhshsn pdlsslivtp
      661 hcegvdapgr dfpehvlkvy kadqtckyll vhrettarev vmlalrefgi tdssslyslc
      721 evsvgeggli kqrrlpeqmq nlaekiglss ryylrnnast dplvpddmas ellresqvsf
      781 lqlnsvevat qltledfsif rqiepteyid dlfevksayg apmlamfael vnremfwvvs
      841 eicgetnpir rmktvkqfvk varqckeckn fnsmfailsg lghgavsrlr stwdklptky
      901 qkmfedlqel mdpsrnmcky rnlinseqtr ppiipfypiv kkdlsfihfg ndtivdnlvn
      961 feklrmiake vrqlmkmcsv pydlysmlea ggsplnpala slnsfaaaav aggagaalar
     1021 rrkrstaqps pkkmfeeaqm vrrvkaylsh lkvvadegrl lamsfeceph papsaipvvl
     1081 psnahpqrkr hpsprkrhps ptlstassns stsegkksin saggvkfgtd spqamrklla
     1141 lsdqskvrph qpprppasps pgvmrrgsat vrdapaavgg asanchgrsh sdagyasvqp
     1201 vdlsaesssv tslnnlplrk shasgtgpps ppivmrnrks mppfthgvpv lpplvpgqhq
     1261 gpmspscllm ppppprdppp vpkgagpprr ppdynvaaqm aarsrqaqrl hrarsqevma
     1321 ataaavaaas sfacevavdp atdheadeed edeaqvsav
//
LOCUS       XP_077506433            1311 aa            linear   INV 25-NOV-2025
DEFINITION  PDZ domain-containing guanine nucleotide exchange factor isoform X3
            [Amblyomma americanum].
ACCESSION   XP_077506433
VERSION     XP_077506433.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650307.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1311
                     /product="PDZ domain-containing guanine nucleotide
                     exchange factor isoform X3"
                     /calculated_mol_wt=143724
     Region          135..245
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(200..201,210..212)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Site            order(233..235,239..241)
                     /site_type="other"
                     /note="flexible hinge region"
                     /db_xref="CDD:237999"
     Region          266..375
                     /region_name="RasGEFN"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     GTPases; N-terminal motif; smart00229"
                     /db_xref="CDD:214571"
     Site            order(285,325..326,329,332..333,368..369,372)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:100121"
     Region          382..465
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cl49608"
                     /db_xref="CDD:483948"
     Site            order(394..400,412,415,445,449,452..453)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467153"
     Region          675..757
                     /region_name="RA_PDZ-GEF1"
                     /note="Ras-associating (RA) domain found in PDZ
                     domain-containing guanine nucleotide exchange factor 1
                     (PDZ-GEF1) and similar proteins; cd01785"
                     /db_xref="CDD:340483"
     Region          780..984
                     /region_name="RasGEF"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     small GTPases. Small GTP-binding proteins of the Ras
                     superfamily function as molecular switches in fundamental
                     events such as signal transduction, cytoskeleton dynamics
                     and intracellular trafficking; cd00155"
                     /db_xref="CDD:238087"
     Site            order(813,824..825,827..829,831..832,835..836,839,872,
                     875..876,878..880,882..885,887..888,908,911,916..918,921,
                     936..938,941..943,945..947,949..952,966,970)
                     /site_type="other"
                     /note="Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:238087"
     CDS             1..1311
                     /gene="PDZ-GEF"
                     /coded_by="XM_077650307.1:360..4295"
                     /db_xref="GeneID:144115811"
ORIGIN      
        1 mnlerllale pdevavetrh phhhlqqlpl dcqqtalhke gllksnrssr ssdtssaysg
       61 sdtmqsvqss lednevdlsg lvestvdsde eedlaesids ltvrdayhec lekdpndrtd
      121 ddietllefm qplpafanmt lsvrrelcav mvfavvekag tvvmndgeel dswsvivngq
      181 vevempdgts htlsmgdsfg itptmekmhh vgtmrtrvdd cqfvciaqgd ycrilhrsee
      241 ntrkleedgr vvlvtehrtv dagsreghiv irgtpdrlla qlfeenaldp gyiedfllty
      301 rtfipsslvv cnkllswfnd pqlrdkvtrv vllwvnnhft dfetdpemme fleqfeqcle
      361 ankmhaqlrl lniacatkar trvvtlarss rddelpfqil ggyetgnaif vakvdkansk
      421 sedgwlkrgd eilevngqsf atmsynqame ilrkathlci tvrsnllefk emlnnpekam
      481 rprgrkssei lrlqpdprlr lsshlewsdv vglplgvggl plgvgwsgqh pssaagspap
      541 atgasvsssg sctgngdvac kedkkgfmtl ghraklkkal akmnfipktl naesqlnssd
      601 dslysthsgs svgsscgnhr vgrsgsspah aiapggcggg ggqlyhshsn pdlsslivtp
      661 hcegvdapgr dfpehvlkvy kadqtckyll vhrettarev vmlalrefgi tdssslyslc
      721 evsvgeggli kqrrlpeqmq nlaekiglss ryylrnnast dplvpddmas ellresqvsf
      781 lqlnsvevat qltledfsif rqiepteyid dlfevksayg apmlamfael vnremfwvvs
      841 eicgetnpir rmktvkqfvk varqckeckn fnsmfailsg lghgavsrlr stwdklptky
      901 qkmfedlqel mdpsrnmcky rnlinseqtr ppiipfypiv kkdlsfihfg ndtivdnlvn
      961 feklrmiake vrqlmkmcsv pydlysmlea ggsplnpala slnsfaaaav aggagaalar
     1021 rrkrstaqps pkkmfeeaqm vrrvkaylsh lkvvadegrl lamsfeceph papsaipvvl
     1081 psnahpqrkr hpsprkrhps ptlstassns stsegkksin saggvkfgtd spqamrklla
     1141 lsdqskvrph qpprppasps pgvmrrgsat vrdapaavgg asanchgrsh sdagyasvqp
     1201 vdlsaesssv tslnnlplrk shasacsvts adsgvgsyqe sdsgrcstgy gspqqrrisg
     1261 tgppsppivm rnrksmppft hgvpvlpplv pgqhqgavad rialsaarft y
//
LOCUS       XP_077506435            1305 aa            linear   INV 25-NOV-2025
DEFINITION  PDZ domain-containing guanine nucleotide exchange factor isoform X4
            [Amblyomma americanum].
ACCESSION   XP_077506435
VERSION     XP_077506435.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650309.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1305
                     /product="PDZ domain-containing guanine nucleotide
                     exchange factor isoform X4"
                     /calculated_mol_wt=143104
     Region          135..245
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(200..201,210..212)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Site            order(233..235,239..241)
                     /site_type="other"
                     /note="flexible hinge region"
                     /db_xref="CDD:237999"
     Region          266..375
                     /region_name="RasGEFN"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     GTPases; N-terminal motif; smart00229"
                     /db_xref="CDD:214571"
     Site            order(285,325..326,329,332..333,368..369,372)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:100121"
     Region          382..465
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cl49608"
                     /db_xref="CDD:483948"
     Site            order(394..400,412,415,445,449,452..453)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467153"
     Region          675..757
                     /region_name="RA_PDZ-GEF1"
                     /note="Ras-associating (RA) domain found in PDZ
                     domain-containing guanine nucleotide exchange factor 1
                     (PDZ-GEF1) and similar proteins; cd01785"
                     /db_xref="CDD:340483"
     Region          780..984
                     /region_name="RasGEF"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     small GTPases. Small GTP-binding proteins of the Ras
                     superfamily function as molecular switches in fundamental
                     events such as signal transduction, cytoskeleton dynamics
                     and intracellular trafficking; cd00155"
                     /db_xref="CDD:238087"
     Site            order(813,824..825,827..829,831..832,835..836,839,872,
                     875..876,878..880,882..885,887..888,908,911,916..918,921,
                     936..938,941..943,945..947,949..952,966,970)
                     /site_type="other"
                     /note="Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:238087"
     CDS             1..1305
                     /gene="PDZ-GEF"
                     /coded_by="XM_077650309.1:360..4277"
                     /db_xref="GeneID:144115811"
ORIGIN      
        1 mnlerllale pdevavetrh phhhlqqlpl dcqqtalhke gllksnrssr ssdtssaysg
       61 sdtmqsvqss lednevdlsg lvestvdsde eedlaesids ltvrdayhec lekdpndrtd
      121 ddietllefm qplpafanmt lsvrrelcav mvfavvekag tvvmndgeel dswsvivngq
      181 vevempdgts htlsmgdsfg itptmekmhh vgtmrtrvdd cqfvciaqgd ycrilhrsee
      241 ntrkleedgr vvlvtehrtv dagsreghiv irgtpdrlla qlfeenaldp gyiedfllty
      301 rtfipsslvv cnkllswfnd pqlrdkvtrv vllwvnnhft dfetdpemme fleqfeqcle
      361 ankmhaqlrl lniacatkar trvvtlarss rddelpfqil ggyetgnaif vakvdkansk
      421 sedgwlkrgd eilevngqsf atmsynqame ilrkathlci tvrsnllefk emlnnpekam
      481 rprgrkssei lrlqpdprlr lsshlewsdv vglplgvggl plgvgwsgqh pssaagspap
      541 atgasvsssg sctgngdvac kedkkgfmtl ghraklkkal akmnfipktl naesqlnssd
      601 dslysthsgs svgsscgnhr vgrsgsspah aiapggcggg ggqlyhshsn pdlsslivtp
      661 hcegvdapgr dfpehvlkvy kadqtckyll vhrettarev vmlalrefgi tdssslyslc
      721 evsvgeggli kqrrlpeqmq nlaekiglss ryylrnnast dplvpddmas ellresqvsf
      781 lqlnsvevat qltledfsif rqiepteyid dlfevksayg apmlamfael vnremfwvvs
      841 eicgetnpir rmktvkqfvk varqckeckn fnsmfailsg lghgavsrlr stwdklptky
      901 qkmfedlqel mdpsrnmcky rnlinseqtr ppiipfypiv kkdlsfihfg ndtivdnlvn
      961 feklrmiake vrqlmkmcsv pydlysmlea ggsplnpala slnsfaaaav aggagaalar
     1021 rrkrstaqps pkkmfeeaqm vrrvkaylsh lkvvadegrl lamsfeceph papsaipvvl
     1081 psnahpqrkr hpsprkrhps ptlstassns stsegkksin saggvkfgtd spqamrklla
     1141 lsdqskvrph qpprppasps pgvmrrgsat vrdapaavgg asanchgrsh sdagyasvqp
     1201 vdlsaesssv tslnnlplrk shasacsvts adsgvgsyqe sdsgrcstgy gspqqrrisg
     1261 tgppsppivm rnrksmppft hgvpvlpplv pgqhqdedea qvsav
//
LOCUS       XP_077506436            1276 aa            linear   INV 25-NOV-2025
DEFINITION  PDZ domain-containing guanine nucleotide exchange factor isoform X5
            [Amblyomma americanum].
ACCESSION   XP_077506436
VERSION     XP_077506436.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650310.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1276
                     /product="PDZ domain-containing guanine nucleotide
                     exchange factor isoform X5"
                     /calculated_mol_wt=140157
     Region          135..245
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(200..201,210..212)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Site            order(233..235,239..241)
                     /site_type="other"
                     /note="flexible hinge region"
                     /db_xref="CDD:237999"
     Region          266..375
                     /region_name="RasGEFN"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     GTPases; N-terminal motif; smart00229"
                     /db_xref="CDD:214571"
     Site            order(285,325..326,329,332..333,368..369,372)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:100121"
     Region          382..465
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cl49608"
                     /db_xref="CDD:483948"
     Site            order(394..400,412,415,445,449,452..453)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467153"
     Region          675..757
                     /region_name="RA_PDZ-GEF1"
                     /note="Ras-associating (RA) domain found in PDZ
                     domain-containing guanine nucleotide exchange factor 1
                     (PDZ-GEF1) and similar proteins; cd01785"
                     /db_xref="CDD:340483"
     Region          780..984
                     /region_name="RasGEF"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     small GTPases. Small GTP-binding proteins of the Ras
                     superfamily function as molecular switches in fundamental
                     events such as signal transduction, cytoskeleton dynamics
                     and intracellular trafficking; cd00155"
                     /db_xref="CDD:238087"
     Site            order(813,824..825,827..829,831..832,835..836,839,872,
                     875..876,878..880,882..885,887..888,908,911,916..918,921,
                     936..938,941..943,945..947,949..952,966,970)
                     /site_type="other"
                     /note="Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:238087"
     CDS             1..1276
                     /gene="PDZ-GEF"
                     /coded_by="XM_077650310.1:293..4123"
                     /db_xref="GeneID:144115811"
ORIGIN      
        1 mnlerllale pdevavetrh phhhlqqlpl dcqqtalhke gllksnrssr ssdtssaysg
       61 sdtmqsvqss lednevdlsg lvestvdsde eedlaesids ltvrdayhec lekdpndrtd
      121 ddietllefm qplpafanmt lsvrrelcav mvfavvekag tvvmndgeel dswsvivngq
      181 vevempdgts htlsmgdsfg itptmekmhh vgtmrtrvdd cqfvciaqgd ycrilhrsee
      241 ntrkleedgr vvlvtehrtv dagsreghiv irgtpdrlla qlfeenaldp gyiedfllty
      301 rtfipsslvv cnkllswfnd pqlrdkvtrv vllwvnnhft dfetdpemme fleqfeqcle
      361 ankmhaqlrl lniacatkar trvvtlarss rddelpfqil ggyetgnaif vakvdkansk
      421 sedgwlkrgd eilevngqsf atmsynqame ilrkathlci tvrsnllefk emlnnpekam
      481 rprgrkssei lrlqpdprlr lsshlewsdv vglplgvggl plgvgwsgqh pssaagspap
      541 atgasvsssg sctgngdvac kedkkgfmtl ghraklkkal akmnfipktl naesqlnssd
      601 dslysthsgs svgsscgnhr vgrsgsspah aiapggcggg ggqlyhshsn pdlsslivtp
      661 hcegvdapgr dfpehvlkvy kadqtckyll vhrettarev vmlalrefgi tdssslyslc
      721 evsvgeggli kqrrlpeqmq nlaekiglss ryylrnnast dplvpddmas ellresqvsf
      781 lqlnsvevat qltledfsif rqiepteyid dlfevksayg apmlamfael vnremfwvvs
      841 eicgetnpir rmktvkqfvk varqckeckn fnsmfailsg lghgavsrlr stwdklptky
      901 qkmfedlqel mdpsrnmcky rnlinseqtr ppiipfypiv kkdlsfihfg ndtivdnlvn
      961 feklrmiake vrqlmkmcsv pydlysmlea ggsplnpala slnsfaaaav aggagaalar
     1021 rrkrstaqps pkkmfeeaqm vrrvkaylsh lkvvadegrl lamsfeceph papsaipvvl
     1081 psnahpqrkr hpsprkrhps ptlstassns stsegkksin saggvkfgtd spqamrklla
     1141 lsdqskvrph qpprppasps pgvmrrgsat vrdapaavgg asanchgrsh sdagyasvqp
     1201 vdlsaesssv tslnnlplrk shasgtgpps ppivmrnrks mppfthgvpv lpplvpgqhq
     1261 gavadrials aarfty
//
LOCUS       XP_077506437            1270 aa            linear   INV 25-NOV-2025
DEFINITION  PDZ domain-containing guanine nucleotide exchange factor isoform X6
            [Amblyomma americanum].
ACCESSION   XP_077506437
VERSION     XP_077506437.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650311.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..1270
                     /product="PDZ domain-containing guanine nucleotide
                     exchange factor isoform X6"
                     /calculated_mol_wt=139537
     Region          135..245
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(200..201,210..212)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Site            order(233..235,239..241)
                     /site_type="other"
                     /note="flexible hinge region"
                     /db_xref="CDD:237999"
     Region          266..375
                     /region_name="RasGEFN"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     GTPases; N-terminal motif; smart00229"
                     /db_xref="CDD:214571"
     Site            order(285,325..326,329,332..333,368..369,372)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:100121"
     Region          382..465
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cl49608"
                     /db_xref="CDD:483948"
     Site            order(394..400,412,415,445,449,452..453)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467153"
     Region          675..757
                     /region_name="RA_PDZ-GEF1"
                     /note="Ras-associating (RA) domain found in PDZ
                     domain-containing guanine nucleotide exchange factor 1
                     (PDZ-GEF1) and similar proteins; cd01785"
                     /db_xref="CDD:340483"
     Region          780..984
                     /region_name="RasGEF"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     small GTPases. Small GTP-binding proteins of the Ras
                     superfamily function as molecular switches in fundamental
                     events such as signal transduction, cytoskeleton dynamics
                     and intracellular trafficking; cd00155"
                     /db_xref="CDD:238087"
     Site            order(813,824..825,827..829,831..832,835..836,839,872,
                     875..876,878..880,882..885,887..888,908,911,916..918,921,
                     936..938,941..943,945..947,949..952,966,970)
                     /site_type="other"
                     /note="Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:238087"
     CDS             1..1270
                     /gene="PDZ-GEF"
                     /coded_by="XM_077650311.1:336..4148"
                     /db_xref="GeneID:144115811"
ORIGIN      
        1 mnlerllale pdevavetrh phhhlqqlpl dcqqtalhke gllksnrssr ssdtssaysg
       61 sdtmqsvqss lednevdlsg lvestvdsde eedlaesids ltvrdayhec lekdpndrtd
      121 ddietllefm qplpafanmt lsvrrelcav mvfavvekag tvvmndgeel dswsvivngq
      181 vevempdgts htlsmgdsfg itptmekmhh vgtmrtrvdd cqfvciaqgd ycrilhrsee
      241 ntrkleedgr vvlvtehrtv dagsreghiv irgtpdrlla qlfeenaldp gyiedfllty
      301 rtfipsslvv cnkllswfnd pqlrdkvtrv vllwvnnhft dfetdpemme fleqfeqcle
      361 ankmhaqlrl lniacatkar trvvtlarss rddelpfqil ggyetgnaif vakvdkansk
      421 sedgwlkrgd eilevngqsf atmsynqame ilrkathlci tvrsnllefk emlnnpekam
      481 rprgrkssei lrlqpdprlr lsshlewsdv vglplgvggl plgvgwsgqh pssaagspap
      541 atgasvsssg sctgngdvac kedkkgfmtl ghraklkkal akmnfipktl naesqlnssd
      601 dslysthsgs svgsscgnhr vgrsgsspah aiapggcggg ggqlyhshsn pdlsslivtp
      661 hcegvdapgr dfpehvlkvy kadqtckyll vhrettarev vmlalrefgi tdssslyslc
      721 evsvgeggli kqrrlpeqmq nlaekiglss ryylrnnast dplvpddmas ellresqvsf
      781 lqlnsvevat qltledfsif rqiepteyid dlfevksayg apmlamfael vnremfwvvs
      841 eicgetnpir rmktvkqfvk varqckeckn fnsmfailsg lghgavsrlr stwdklptky
      901 qkmfedlqel mdpsrnmcky rnlinseqtr ppiipfypiv kkdlsfihfg ndtivdnlvn
      961 feklrmiake vrqlmkmcsv pydlysmlea ggsplnpala slnsfaaaav aggagaalar
     1021 rrkrstaqps pkkmfeeaqm vrrvkaylsh lkvvadegrl lamsfeceph papsaipvvl
     1081 psnahpqrkr hpsprkrhps ptlstassns stsegkksin saggvkfgtd spqamrklla
     1141 lsdqskvrph qpprppasps pgvmrrgsat vrdapaavgg asanchgrsh sdagyasvqp
     1201 vdlsaesssv tslnnlplrk shasgtgpps ppivmrnrks mppfthgvpv lpplvpgqhq
     1261 dedeaqvsav
//
LOCUS       XP_077506438             112 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115812 [Amblyomma americanum].
ACCESSION   XP_077506438
VERSION     XP_077506438.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650312.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..112
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..112
                     /product="uncharacterized protein LOC144115812"
                     /calculated_mol_wt=12050
     CDS             1..112
                     /gene="LOC144115812"
                     /coded_by="XM_077650312.1:2994..3332"
                     /db_xref="GeneID:144115812"
ORIGIN      
        1 mterftrgsg rplvtkqksm ddvlselqgn amflsfgkrs ggtgrrmnec lilepsemvv
       61 vsallafpvr swaaagveca stseprlgvv ahfapvtapm ccychsvrvs rr
//
LOCUS       XP_077506439             366 aa            linear   INV 25-NOV-2025
DEFINITION  heparan sulfate 2-O-sulfotransferase [Amblyomma americanum].
ACCESSION   XP_077506439
VERSION     XP_077506439.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650313.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..366
                     /product="heparan sulfate 2-O-sulfotransferase"
                     /calculated_mol_wt=42877
     Region          83..331
                     /region_name="Sulfotransfer_2"
                     /note="Sulfotransferase family; pfam03567"
                     /db_xref="CDD:427369"
     CDS             1..366
                     /gene="Hs2st"
                     /coded_by="XM_077650313.1:130..1230"
                     /db_xref="GeneID:144115814"
ORIGIN      
        1 migricqwkv ilcfciivcf asvflrfenk lavlessrlk ltdtvgrlql fylnerlhdf
       61 ppvqeshtda plddfglpek tmdnlvilyn rvpktgstsf mgvaydlcat nkfhvlhlnt
      121 sknmhvmslp dqirfvynis lwhymkpaiy hghiaflnfa kygvtqrpiy inlirrpldr
      181 lvsyfyflrh gddfrpylvr rrqgnkmtfd eciatkgadc aeerlwlqvp ffcghaarcw
      241 ipgnpwaleq akhnlvnhyf lvglteqlqe fvamlevsfp rifkgatnkf lsgrrshlrk
      301 tfnkvqpsee tvehfkqsai wqmenefyef aaeqfefakk rtlvatrdgq ltelgqqffy
      361 ekirpk
//
LOCUS       XP_077506440             633 aa            linear   INV 25-NOV-2025
DEFINITION  formin-binding protein 1-like Cip4 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506440
VERSION     XP_077506440.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650314.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..633
                     /product="formin-binding protein 1-like Cip4 isoform X1"
                     /calculated_mol_wt=71127
     Region          66..312
                     /region_name="F-BAR_CIP4-like"
                     /note="The F-BAR (FES-CIP4 Homology and
                     Bin/Amphiphysin/Rvs) domain of Cdc42-Interacting Protein 4
                     and similar proteins; cd07653"
                     /db_xref="CDD:153337"
     Site            order(67,70,77,80..81,84,87..88,91..92,95,99,102,106,135,
                     138,242,246,272,276,279,282..283,286,290,293,297,301,
                     304..305,308)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153337"
     Site            order(90,101,108..109,113,165,168..169,187,194,198,201,
                     205)
                     /site_type="other"
                     /note="putative membrane interaction site"
                     /db_xref="CDD:153337"
     Region          406..482
                     /region_name="HR1_CIP4-like"
                     /note="Protein kinase C-related kinase homology region 1
                     (HR1) Rho-binding domain of Cdc42-Interacting Protein 4
                     and similar proteins; cd11619"
                     /db_xref="CDD:212009"
     Site            order(418,421,424..425,428..429,431..432,435..436,
                     438..439,457..458,461,464..465,468)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212009"
     Site            order(426..427,429..430,433..434,436..437,440..441,
                     452..453,455)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212009"
     Region          573..627
                     /region_name="SH3_CIP4-like"
                     /note="Src Homology 3 domain of Cdc42-Interacting Protein
                     4; cd11911"
                     /db_xref="CDD:212844"
     Site            order(578,580,583,587,606..607,621,623..624)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212844"
     CDS             1..633
                     /gene="Cip4"
                     /coded_by="XM_077650314.1:449..2350"
                     /db_xref="GeneID:144115816"
ORIGIN      
        1 mcsswsagaa eparakcrlv lsyvgtlrrr ppsasprywa eqqhdedvri yrrpwtsghh
       61 hlhcrdqydk isqhthqglq flenfsqfik drstietdya arlrklaksy qpkkkddedn
      121 gytsskafcl vlnevndiag qheliaenls thinrevatl ikelkeerkr llsegsklqt
      181 alqnsvtsld kskkyyekmf reaekaqeaf akadadlqls radlekakqn slaknkaced
      241 sktdyanqlq ktnalqrkhy telmpkvfeq lqslderrta slqgyikkaa eihrevipii
      301 lkcldgivva adsidpkkda etvveryksg fvpaddfpfe dlsvtrncds ssttgstvns
      361 sfrgagdtvr gtistgkkkr sglfgifssn knnidefkdd ysnlppnqrr kkllqelekt
      421 raqintltqa reglyklkgs yeenaalgdp qtvqgeindi gaklekyqtd lekyqnylte
      481 vdsqmatpdt qkryrnsfse gslsrsases svsnnhqnnn kgpstpitah naacrpesgl
      541 gtshtsipdd dgdfengyev dsfeaellpa lgkavaiyaf daqsegsipm eegeeflvve
      601 vdqgdgwtrv rrenleegfv ptsylectmf nsc
//
LOCUS       XP_077506441             626 aa            linear   INV 25-NOV-2025
DEFINITION  formin-binding protein 1-like Cip4 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506441
VERSION     XP_077506441.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650315.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..626
                     /product="formin-binding protein 1-like Cip4 isoform X2"
                     /calculated_mol_wt=70266
     Region          66..312
                     /region_name="F-BAR_CIP4-like"
                     /note="The F-BAR (FES-CIP4 Homology and
                     Bin/Amphiphysin/Rvs) domain of Cdc42-Interacting Protein 4
                     and similar proteins; cd07653"
                     /db_xref="CDD:153337"
     Site            order(67,70,77,80..81,84,87..88,91..92,95,99,102,106,135,
                     138,242,246,272,276,279,282..283,286,290,293,297,301,
                     304..305,308)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153337"
     Site            order(90,101,108..109,113,165,168..169,187,194,198,201,
                     205)
                     /site_type="other"
                     /note="putative membrane interaction site"
                     /db_xref="CDD:153337"
     Region          399..475
                     /region_name="HR1_CIP4-like"
                     /note="Protein kinase C-related kinase homology region 1
                     (HR1) Rho-binding domain of Cdc42-Interacting Protein 4
                     and similar proteins; cd11619"
                     /db_xref="CDD:212009"
     Site            order(411,414,417..418,421..422,424..425,428..429,
                     431..432,450..451,454,457..458,461)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212009"
     Site            order(419..420,422..423,426..427,429..430,433..434,
                     445..446,448)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212009"
     Region          566..620
                     /region_name="SH3_CIP4-like"
                     /note="Src Homology 3 domain of Cdc42-Interacting Protein
                     4; cd11911"
                     /db_xref="CDD:212844"
     Site            order(571,573,576,580,599..600,614,616..617)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212844"
     CDS             1..626
                     /gene="Cip4"
                     /coded_by="XM_077650315.1:487..2367"
                     /db_xref="GeneID:144115816"
ORIGIN      
        1 mcsswsagaa eparakcrlv lsyvgtlrrr ppsasprywa eqqhdedvri yrrpwtsghh
       61 hlhcrdqydk isqhthqglq flenfsqfik drstietdya arlrklaksy qpkkkddedn
      121 gytsskafcl vlnevndiag qheliaenls thinrevatl ikelkeerkr llsegsklqt
      181 alqnsvtsld kskkyyekmf reaekaqeaf akadadlqls radlekakqn slaknkaced
      241 sktdyanqlq ktnalqrkhy telmpkvfeq lqslderrta slqgyikkaa eihrevipii
      301 lkcldgivva adsidpkkda etvveryksg fvpaddfpfe dlsvtrncds ssttgstvns
      361 sfrgagdtvr gtistgkkkr sglfgifssn kddysnlppn qrrkkllqel ektraqintl
      421 tqareglykl kgsyeenaal gdpqtvqgei ndigakleky qtdlekyqny ltevdsqmat
      481 pdtqkryrns fsegslsrsa sessvsnnhq nnnkgpstpi tahnaacrpe sglgtshtsi
      541 pdddgdfeng yevdsfeael lpalgkavai yafdaqsegs ipmeegeefl vvevdqgdgw
      601 trvrrenlee gfvptsylec tmfnsc
//
LOCUS       XP_077506442             576 aa            linear   INV 25-NOV-2025
DEFINITION  formin-binding protein 1-like Cip4 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077506442
VERSION     XP_077506442.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650316.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..576
                     /product="formin-binding protein 1-like Cip4 isoform X3"
                     /calculated_mol_wt=64563
     Region          6..255
                     /region_name="F-BAR_CIP4-like"
                     /note="The F-BAR (FES-CIP4 Homology and
                     Bin/Amphiphysin/Rvs) domain of Cdc42-Interacting Protein 4
                     and similar proteins; cd07653"
                     /db_xref="CDD:153337"
     Site            order(10,13,20,23..24,27,30..31,34..35,38,42,45,49,78,81,
                     185,189,215,219,222,225..226,229,233,236,240,244,247..248,
                     251)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153337"
     Site            order(33,44,51..52,56,108,111..112,130,137,141,144,148)
                     /site_type="other"
                     /note="putative membrane interaction site"
                     /db_xref="CDD:153337"
     Region          349..425
                     /region_name="HR1_CIP4-like"
                     /note="Protein kinase C-related kinase homology region 1
                     (HR1) Rho-binding domain of Cdc42-Interacting Protein 4
                     and similar proteins; cd11619"
                     /db_xref="CDD:212009"
     Site            order(361,364,367..368,371..372,374..375,378..379,
                     381..382,400..401,404,407..408,411)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212009"
     Site            order(369..370,372..373,376..377,379..380,383..384,
                     395..396,398)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212009"
     Region          516..570
                     /region_name="SH3_CIP4-like"
                     /note="Src Homology 3 domain of Cdc42-Interacting Protein
                     4; cd11911"
                     /db_xref="CDD:212844"
     Site            order(521,523,526,530,549..550,564,566..567)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212844"
     CDS             1..576
                     /gene="Cip4"
                     /coded_by="XM_077650316.1:214..1944"
                     /db_xref="GeneID:144115816"
ORIGIN      
        1 mswglelwdq ydkisqhthq glqflenfsq fikdrstiet dyaarlrkla ksyqpkkkdd
       61 edngytsska fclvlnevnd iagqheliae nlsthinrev atlikelkee rkrllsegsk
      121 lqtalqnsvt sldkskkyye kmfreaekaq eafakadadl qlsradleka kqnslaknka
      181 cedsktdyan qlqktnalqr khytelmpkv feqlqslder rtaslqgyik kaaeihrevi
      241 piilkcldgi vvaadsidpk kdaetvvery ksgfvpaddf pfedlsvtrn cdsssttgst
      301 vnssfrgagd tvrgtistgk kkrsglfgif ssnknnidef kddysnlppn qrrkkllqel
      361 ektraqintl tqareglykl kgsyeenaal gdpqtvqgei ndigakleky qtdlekyqny
      421 ltevdsqmat pdtqkryrns fsegslsrsa sessvsnnhq nnnkgpstpi tahnaacrpe
      481 sglgtshtsi pdddgdfeng yevdsfeael lpalgkavai yafdaqsegs ipmeegeefl
      541 vvevdqgdgw trvrrenlee gfvptsylec tmfnsc
//
LOCUS       XP_077506443             571 aa            linear   INV 25-NOV-2025
DEFINITION  formin-binding protein 1-like Cip4 isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077506443
VERSION     XP_077506443.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650317.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..571
                     /product="formin-binding protein 1-like Cip4 isoform X4"
                     /calculated_mol_wt=63959
     Region          4..250
                     /region_name="F-BAR_CIP4-like"
                     /note="The F-BAR (FES-CIP4 Homology and
                     Bin/Amphiphysin/Rvs) domain of Cdc42-Interacting Protein 4
                     and similar proteins; cd07653"
                     /db_xref="CDD:153337"
     Site            order(5,8,15,18..19,22,25..26,29..30,33,37,40,44,73,76,
                     180,184,210,214,217,220..221,224,228,231,235,239,242..243,
                     246)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153337"
     Site            order(28,39,46..47,51,103,106..107,125,132,136,139,143)
                     /site_type="other"
                     /note="putative membrane interaction site"
                     /db_xref="CDD:153337"
     Region          344..420
                     /region_name="HR1_CIP4-like"
                     /note="Protein kinase C-related kinase homology region 1
                     (HR1) Rho-binding domain of Cdc42-Interacting Protein 4
                     and similar proteins; cd11619"
                     /db_xref="CDD:212009"
     Site            order(356,359,362..363,366..367,369..370,373..374,
                     376..377,395..396,399,402..403,406)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212009"
     Site            order(364..365,367..368,371..372,374..375,378..379,
                     390..391,393)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212009"
     Region          511..565
                     /region_name="SH3_CIP4-like"
                     /note="Src Homology 3 domain of Cdc42-Interacting Protein
                     4; cd11911"
                     /db_xref="CDD:212844"
     Site            order(516,518,521,525,544..545,559,561..562)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212844"
     CDS             1..571
                     /gene="Cip4"
                     /coded_by="XM_077650317.1:292..2007"
                     /db_xref="GeneID:144115816"
ORIGIN      
        1 mhmdqydkis qhthqglqfl enfsqfikdr stietdyaar lrklaksyqp kkkddedngy
       61 tsskafclvl nevndiagqh eliaenlsth inrevatlik elkeerkrll segsklqtal
      121 qnsvtsldks kkyyekmfre aekaqeafak adadlqlsra dlekakqnsl aknkacedsk
      181 tdyanqlqkt nalqrkhyte lmpkvfeqlq slderrtasl qgyikkaaei hrevipiilk
      241 cldgivvaad sidpkkdaet vveryksgfv paddfpfedl svtrncdsss ttgstvnssf
      301 rgagdtvrgt istgkkkrsg lfgifssnkn nidefkddys nlppnqrrkk llqelektra
      361 qintltqare glyklkgsye enaalgdpqt vqgeindiga klekyqtdle kyqnyltevd
      421 sqmatpdtqk ryrnsfsegs lsrsasessv snnhqnnnkg pstpitahna acrpesglgt
      481 shtsipdddg dfengyevds feaellpalg kavaiyafda qsegsipmee geeflvvevd
      541 qgdgwtrvrr enleegfvpt sylectmfns c
//
LOCUS       XP_077506444             569 aa            linear   INV 25-NOV-2025
DEFINITION  formin-binding protein 1-like Cip4 isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077506444
VERSION     XP_077506444.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650318.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..569
                     /product="formin-binding protein 1-like Cip4 isoform X5"
                     /calculated_mol_wt=63702
     Region          6..255
                     /region_name="F-BAR_CIP4-like"
                     /note="The F-BAR (FES-CIP4 Homology and
                     Bin/Amphiphysin/Rvs) domain of Cdc42-Interacting Protein 4
                     and similar proteins; cd07653"
                     /db_xref="CDD:153337"
     Site            order(10,13,20,23..24,27,30..31,34..35,38,42,45,49,78,81,
                     185,189,215,219,222,225..226,229,233,236,240,244,247..248,
                     251)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153337"
     Site            order(33,44,51..52,56,108,111..112,130,137,141,144,148)
                     /site_type="other"
                     /note="putative membrane interaction site"
                     /db_xref="CDD:153337"
     Region          342..418
                     /region_name="HR1_CIP4-like"
                     /note="Protein kinase C-related kinase homology region 1
                     (HR1) Rho-binding domain of Cdc42-Interacting Protein 4
                     and similar proteins; cd11619"
                     /db_xref="CDD:212009"
     Site            order(354,357,360..361,364..365,367..368,371..372,
                     374..375,393..394,397,400..401,404)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212009"
     Site            order(362..363,365..366,369..370,372..373,376..377,
                     388..389,391)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212009"
     Region          509..563
                     /region_name="SH3_CIP4-like"
                     /note="Src Homology 3 domain of Cdc42-Interacting Protein
                     4; cd11911"
                     /db_xref="CDD:212844"
     Site            order(514,516,519,523,542..543,557,559..560)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212844"
     CDS             1..569
                     /gene="Cip4"
                     /coded_by="XM_077650318.1:214..1923"
                     /db_xref="GeneID:144115816"
ORIGIN      
        1 mswglelwdq ydkisqhthq glqflenfsq fikdrstiet dyaarlrkla ksyqpkkkdd
       61 edngytsska fclvlnevnd iagqheliae nlsthinrev atlikelkee rkrllsegsk
      121 lqtalqnsvt sldkskkyye kmfreaekaq eafakadadl qlsradleka kqnslaknka
      181 cedsktdyan qlqktnalqr khytelmpkv feqlqslder rtaslqgyik kaaeihrevi
      241 piilkcldgi vvaadsidpk kdaetvvery ksgfvpaddf pfedlsvtrn cdsssttgst
      301 vnssfrgagd tvrgtistgk kkrsglfgif ssnkddysnl ppnqrrkkll qelektraqi
      361 ntltqaregl yklkgsyeen aalgdpqtvq geindigakl ekyqtdleky qnyltevdsq
      421 matpdtqkry rnsfsegsls rsasessvsn nhqnnnkgps tpitahnaac rpesglgtsh
      481 tsipdddgdf engyevdsfe aellpalgka vaiyafdaqs egsipmeege eflvvevdqg
      541 dgwtrvrren leegfvptsy lectmfnsc
//
LOCUS       XP_077506446             571 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115819 [Amblyomma americanum].
ACCESSION   XP_077506446
VERSION     XP_077506446.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650320.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..571
                     /product="uncharacterized protein LOC144115819"
                     /calculated_mol_wt=62952
     CDS             1..571
                     /gene="LOC144115819"
                     /coded_by="XM_077650320.1:181..1896"
                     /db_xref="GeneID:144115819"
ORIGIN      
        1 mprakkafvr refhgnryah rekakgcprp neipnaeras sstsklsrfs lcfqeedvlh
       61 sssqyalmdm dgicaaitqi cvcrecggsv elieivtkrv gvasvyslnc avcsaaqrfe
      121 tskkttsgly eanlrlvyal rsigkgmaag nilcamlnlp kpptkfakyn qellghieaa
      181 aqesmkraad eavqlnegdk diavaldgsw qkrghtsnng ivsatsvdsg kvldvevlfk
      241 rcpkcsikgt nsdplhrevc qsnyqgtsgg mevagalkif grseelhgvr yvkylgdgds
      301 kafmavkaqm pygdsveisk vecighvqkr mgtrlrrlkk gnkagklsdg kslsgrgrlt
      361 davidklqty yglairrnvg nldemrkavw atffhlsatd ddpchglcpk gpdtwcafnr
      421 sqsegkpffh keslpasvle aikpiyrels kaellekclh grtqnanesf npvvwerapk
      481 nvfvrlrtlr matldavlsf ndgsiaranv lkacglnpgs ntikwlkead hkrmyfadra
      541 trqltkearq skrqaekrkn dgdsdyeagg y
//
LOCUS       XP_077506447             687 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115821 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506447
VERSION     XP_077506447.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650321.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..687
                     /product="uncharacterized protein LOC144115821 isoform X1"
                     /calculated_mol_wt=74564
     Region          86..285
                     /region_name="DAXX_histone_binding"
                     /note="Histone binding domain of the death-domain
                     associated protein (DAXX); cd13150"
                     /db_xref="CDD:240523"
     Site            order(96,98,101,104..105,107..113,115,117..119,121..124,
                     126..127,130,133..134,137,150..152,181..185,188,215,
                     219..220,223,226,229..230,233..234,236..238,240,267,
                     270..271,274..275,277..278,281..282,285)
                     /site_type="other"
                     /note="Histone H3.3 interface [polypeptide binding]"
                     /db_xref="CDD:240523"
     Site            order(111,181,183,186,227,230..231,234,239,243,247..249,
                     252,255,259,262,266,269..270,273..274,277,281)
                     /site_type="other"
                     /note="Histone H4 interface [polypeptide binding]"
                     /db_xref="CDD:240523"
     CDS             1..687
                     /gene="LOC144115821"
                     /coded_by="XM_077650321.1:47..2110"
                     /db_xref="GeneID:144115821"
ORIGIN      
        1 maelgalear aeskrpeiit isddeevpes teskkpidvn leefrtenkk pidvileefk
       61 tliqpvpkdk gntfnraeyq vspvltkrtq esnrvmfkel crsirqlree evdfnedded
      121 sayiqedrlt krawnvwrkl cklegrhpnt gceqykkfhf tgtiypaina everlvnnri
      181 fpdftdirnl vkraneqedl gldrketeti aeevftsvgr elqrrrkrde lnsamayldd
      241 ygdygrdpak ndpelrakle entnvyqkrm ddviqayvnk qqelklepqe vgeedcdrsp
      301 eqdssnlsdd eenekgsied dkktpekdll ensifnegkq gveegggevc kplcaagspa
      361 sltgedssks pvagggsgla ltrgsysase lsikpiperp lssgpsggss riaqlsispv
      421 cggsaaadla iipvpttrmr kaeltitavp vasesprvti spvpiasgkq gveegggevc
      481 kplcaagspa sltgedssks pvagggsgla ltrgsysase lsikpiperp lssgpsggss
      541 riaqlsispv cggsaaadla iipvpttrmr kaeltitavp vasesprvti spvpiasgks
      601 klsmslgaed qqrhgtengs ilmpevsifp vhcdklsprp kdcsrgqnlp vkrrrsspek
      661 dpegdiiiil sddevpappa ktfkrle
//
LOCUS       XP_077506448             670 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115821 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506448
VERSION     XP_077506448.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650322.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..670
                     /product="uncharacterized protein LOC144115821 isoform X2"
                     /calculated_mol_wt=72742
     Region          69..268
                     /region_name="DAXX_histone_binding"
                     /note="Histone binding domain of the death-domain
                     associated protein (DAXX); cd13150"
                     /db_xref="CDD:240523"
     Site            order(79,81,84,87..88,90..96,98,100..102,104..107,
                     109..110,113,116..117,120,133..135,164..168,171,198,
                     202..203,206,209,212..213,216..217,219..221,223,250,
                     253..254,257..258,260..261,264..265,268)
                     /site_type="other"
                     /note="Histone H3.3 interface [polypeptide binding]"
                     /db_xref="CDD:240523"
     Site            order(94,164,166,169,210,213..214,217,222,226,230..232,
                     235,238,242,245,249,252..253,256..257,260,264)
                     /site_type="other"
                     /note="Histone H4 interface [polypeptide binding]"
                     /db_xref="CDD:240523"
     CDS             1..670
                     /gene="LOC144115821"
                     /coded_by="XM_077650322.1:139..2151"
                     /db_xref="GeneID:144115821"
ORIGIN      
        1 maefgrietr aegkrpeiit iyeevpeste nkkpidvile efktliqpvp kdkgntfnra
       61 eyqvspvltk rtqesnrvmf kelcrsirql reeevdfned dedsayiqed rltkrawnvw
      121 rklcklegrh pntgceqykk fhftgtiypa inaeverlvn nrifpdftdi rnlvkraneq
      181 edlgldrket etiaeevfts vgrelqrrrk rdelnsamay lddygdygrd pakndpelra
      241 kleentnvyq krmddviqay vnkqqelkle pqevgeedcd rspeqdssnl sddeenekgs
      301 ieddkktpek dllensifne gkqgveeggg evckplcaag spasltgeds skspvagggs
      361 glaltrgsys aselsikpip erplssgpsg gssriaqlsi spvcggsaaa dlaiipvptt
      421 rmrkaeltit avpvasespr vtispvpias gkqgveeggg evckplcaag spasltgeds
      481 skspvagggs glaltrgsys aselsikpip erplssgpsg gssriaqlsi spvcggsaaa
      541 dlaiipvptt rmrkaeltit avpvasespr vtispvpias gksklsmslg aedqqrhgte
      601 ngsilmpevs ifpvhcdkls prpkdcsrgq nlpvkrrrss pekdpegdii iilsddevpa
      661 ppaktfkrle
//
LOCUS       XP_077506449             617 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115821 isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077506449
VERSION     XP_077506449.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650323.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..617
                     /product="uncharacterized protein LOC144115821 isoform X3"
                     /calculated_mol_wt=66405
     Region          19..215
                     /region_name="DAXX_histone_binding"
                     /note="Histone binding domain of the death-domain
                     associated protein (DAXX); cd13150"
                     /db_xref="CDD:240523"
     Site            order(28..29,32,34..35,37..43,45,47..49,51..54,56..57,60,
                     63..64,67,80..82,111..115,118,145,149..150,153,156,
                     159..160,163..164,166..168,170,197,200..201,204..205,
                     207..208,211..212,215)
                     /site_type="other"
                     /note="Histone H3.3 interface [polypeptide binding]"
                     /db_xref="CDD:240523"
     Site            order(41,111,113,116,157,160..161,164,169,173,177..179,
                     182,185,189,192,196,199..200,203..204,207,211)
                     /site_type="other"
                     /note="Histone H4 interface [polypeptide binding]"
                     /db_xref="CDD:240523"
     CDS             1..617
                     /gene="LOC144115821"
                     /coded_by="XM_077650323.1:446..2299"
                     /db_xref="GeneID:144115821"
ORIGIN      
        1 malavqgraa vchvetfgni qklqeyllel crsirqlree evdfnedded sayiqedrlt
       61 krawnvwrkl cklegrhpnt gceqykkfhf tgtiypaina everlvnnri fpdftdirnl
      121 vkraneqedl gldrketeti aeevftsvgr elqrrrkrde lnsamayldd ygdygrdpak
      181 ndpelrakle entnvyqkrm ddviqayvnk qqelklepqe vgeedcdrsp eqdssnlsdd
      241 eenekgsied dkktpekdll ensifnegkq gveegggevc kplcaagspa sltgedssks
      301 pvagggsgla ltrgsysase lsikpiperp lssgpsggss riaqlsispv cggsaaadla
      361 iipvpttrmr kaeltitavp vasesprvti spvpiasgkq gveegggevc kplcaagspa
      421 sltgedssks pvagggsgla ltrgsysase lsikpiperp lssgpsggss riaqlsispv
      481 cggsaaadla iipvpttrmr kaeltitavp vasesprvti spvpiasgks klsmslgaed
      541 qqrhgtengs ilmpevsifp vhcdklsprp kdcsrgqnlp vkrrrsspek dpegdiiiil
      601 sddevpappa ktfkrle
//
LOCUS       XP_077506450             276 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115822 [Amblyomma americanum].
ACCESSION   XP_077506450
VERSION     XP_077506450.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650324.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..276
                     /product="uncharacterized protein LOC144115822"
                     /calculated_mol_wt=31888
     CDS             1..276
                     /gene="LOC144115822"
                     /coded_by="XM_077650324.1:79..909"
                     /db_xref="GeneID:144115822"
ORIGIN      
        1 mkvrsfscdl cpkhfvilrd lrrhhvdehn feeevenlvf dsntefctwk dgeesrtcrh
       61 fiastgrkes assvfkrylh chrsgthkel pieqrqrqkk gqgscrigkh cfatlevtek
      121 dskvyvvfqk khyghfnevc hqrlsqdear vlagrlaegv paktvvhnir agtsgssprm
      181 qlvttkdlrn iadkfrigke eqrdpddfks vdlwveemra enespllyym kkgdpnshdg
      241 dfelalmtfp qrellsklgt eklcidsthg tnkykf
//
LOCUS       XP_077506451             276 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115822 [Amblyomma americanum].
ACCESSION   XP_077506451
VERSION     XP_077506451.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650325.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..276
                     /product="uncharacterized protein LOC144115822"
                     /calculated_mol_wt=31888
     CDS             1..276
                     /gene="LOC144115822"
                     /coded_by="XM_077650325.1:79..909"
                     /db_xref="GeneID:144115822"
ORIGIN      
        1 mkvrsfscdl cpkhfvilrd lrrhhvdehn feeevenlvf dsntefctwk dgeesrtcrh
       61 fiastgrkes assvfkrylh chrsgthkel pieqrqrqkk gqgscrigkh cfatlevtek
      121 dskvyvvfqk khyghfnevc hqrlsqdear vlagrlaegv paktvvhnir agtsgssprm
      181 qlvttkdlrn iadkfrigke eqrdpddfks vdlwveemra enespllyym kkgdpnshdg
      241 dfelalmtfp qrellsklgt eklcidsthg tnkykf
//
LOCUS       XP_077506453             786 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115825 [Amblyomma americanum].
ACCESSION   XP_077506453
VERSION     XP_077506453.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650327.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..786
                     /product="uncharacterized protein LOC144115825"
                     /calculated_mol_wt=89741
     CDS             1..786
                     /gene="LOC144115825"
                     /coded_by="XM_077650327.1:151..2511"
                     /db_xref="GeneID:144115825"
ORIGIN      
        1 mpgirffrcp rcachqfslr tlfrhlriah ghetnwvcgl sgcmqtfrhf fsykkhvhrk
       61 hascmgetcs rgivqvgmar sstnaapdlp tyheepldvq aeqhdfsssd ygyqlakllf
      121 kwkegrrlpe stvhelandi idfvetiadh qqtepldgpa isvkeicklq ldqlrtrsgr
      181 ttywkthfpf vesrtvvlcg ndtieyiplc evlkcilehs eltnefsnds rtdgymcsvf
      241 dgsafqnhey fagntsklcl qlysdefevc nplgskrgkh kmtavyfsvl nfdaklrsal
      301 sgihlvllvk dkhiatygfs kileplitdv kilanhgitv nrnlvkgsvf iftgdnlssh
      361 riggfkctfs qgricrycma lrtetdykhl egdfvlrspq ghkhhlsmlr aglptlslyg
      421 vkepsallcp gfdptqhlpp dimhdlhegv lpfalrhiis alieerffsl sdlnksisew
      481 lydphdicnk peaiakpylq gkavlkasat qvfclfrylt ffvgdnvpsd navwqlylll
      541 reivdiimsh kipvshiayl qrkihffcfd fkvlfpsasl pckvhylihy psyiekfgpl
      601 sllwamrfea khqyfkdiar kirnwknlsh slavrhqflq sflfaagdds iprnigvksv
      661 lyehlpgcvk efitennles tntlvvksvi vdgrtysagc clvrsvpeds lpefllvcql
      721 fyvnkvlvvm aklletvcfd ehfhvyvvsa ieeysvltsf sefdieplym rehkgrsvvs
      781 trhslf
//
LOCUS       XP_077506454             156 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115826 [Amblyomma americanum].
ACCESSION   XP_077506454
VERSION     XP_077506454.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650328.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 21% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..156
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..156
                     /product="uncharacterized protein LOC144115826"
                     /calculated_mol_wt=16331
     CDS             1..156
                     /gene="LOC144115826"
                     /coded_by="XM_077650328.1:33..503"
                     /db_xref="GeneID:144115826"
ORIGIN      
        1 mlgqpkqksk eedarkkrdr hktgggsaqc tvsaqseqvi avashimtrv gnqtdsdggi
       61 dlppvaslpv irllqpmvdg agneefhyae ddwepplvde pqspaaavgk sgatpeaieq
      121 esnhffpavq padasstaaa gcskcdssya vykvdp
//
LOCUS       XP_077506455             114 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115828 [Amblyomma americanum].
ACCESSION   XP_077506455
VERSION     XP_077506455.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650329.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..114
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..114
                     /product="uncharacterized protein LOC144115828"
                     /calculated_mol_wt=12434
     CDS             1..114
                     /gene="LOC144115828"
                     /coded_by="XM_077650329.1:55..399"
                     /db_xref="GeneID:144115828"
ORIGIN      
        1 masdddlgdl pscpficvkg wtfltansyt icvdthpvlh askpdeafkl lffahfafni
       61 qyqketslcl eftqralagi npargtkvqk nggkqhclsp rvaalatalk dydl
//
LOCUS       XP_077506456             695 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115829 [Amblyomma americanum].
ACCESSION   XP_077506456
VERSION     XP_077506456.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650330.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..695
                     /product="uncharacterized protein LOC144115829"
                     /calculated_mol_wt=77739
     Region          237..380
                     /region_name="Transposase_21"
                     /note="Transposase family tnp2; cl29371"
                     /db_xref="CDD:475188"
     CDS             1..695
                     /gene="LOC144115829"
                     /coded_by="XM_077650330.1:147..2234"
                     /db_xref="GeneID:144115829"
ORIGIN      
        1 msmetevmsk wrryriieke yrellgelgi rrppsthlks sgskrtggea aldvvsappl
       61 plpsgaskrp nseayasgac asgsgratss lpadpsmpsv qselneqayv sescaeslss
      121 ehtatscdss detsstnslt aenvemgqpr eespelcyrh lstaqelasi askhnlthac
      181 indlldfcrr rgivelpkda rtimqterka nlehgdsfvh fglaegiqha vgqgpapenv
      241 elhanidglp lhrssqtalw pilcrvinik ksepfmvsay cgagkppclq eylrpfidev
      301 rnlscnglmv kgvhvnvclk avicdapars fvkaitghtg yhacercsqk ghylenrmtf
      361 pdlhapqrtd ssfrsqedrr hhtgvspfts lnvdmvkcfp teymhlvclg vmrrllrnwi
      421 ckghaarlsr adrsalndkl reaatafpky fqrkprgtee ldrwkatefr tfllylgpvl
      481 lksilpdely khflylhvsv rvlaspqhhr dlnqfahdll ryfvqefgrl ygqkqlvynv
      541 htlahlaeqc lehgtvdefs afpfesylgk vkkrlrttnk plaqlsrrms evractplst
      601 gqvggdvkvg dcflvdncav vivdllggna kagilpngre ffrvpidssr mdvrrysalg
      661 qetkiwpisy iknkvrcikl qykrghvvfp llhlq
//
LOCUS       XP_077506457             110 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115830 [Amblyomma americanum].
ACCESSION   XP_077506457
VERSION     XP_077506457.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650331.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..110
                     /product="uncharacterized protein LOC144115830"
                     /calculated_mol_wt=12103
     CDS             1..110
                     /gene="LOC144115830"
                     /coded_by="XM_077650331.1:1236..1568"
                     /db_xref="GeneID:144115830"
ORIGIN      
        1 mtwgdlpscp ficakgwtfl tansyticvd thpvlhaskp deafkllffa hfafniqyqk
       61 etslcleftq raiaginppr gtkvqknggk qhclsprvaa latalkdydi
//
LOCUS       XP_077506458             110 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115830 [Amblyomma americanum].
ACCESSION   XP_077506458
VERSION     XP_077506458.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650332.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..110
                     /product="uncharacterized protein LOC144115830"
                     /calculated_mol_wt=12103
     CDS             1..110
                     /gene="LOC144115830"
                     /coded_by="XM_077650332.1:114..446"
                     /db_xref="GeneID:144115830"
ORIGIN      
        1 mtwgdlpscp ficakgwtfl tansyticvd thpvlhaskp deafkllffa hfafniqyqk
       61 etslcleftq raiaginppr gtkvqknggk qhclsprvaa latalkdydi
//
LOCUS       XP_077506459             100 aa            linear   INV 25-NOV-2025
DEFINITION  ixochymostatin-like [Amblyomma americanum].
ACCESSION   XP_077506459
VERSION     XP_077506459.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650333.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..100
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..100
                     /product="ixochymostatin-like"
                     /calculated_mol_wt=10780
     Region          41..97
                     /region_name="TIL"
                     /note="trypsin inhibitor-like cysteine rich domain;
                     cd19941"
                     /db_xref="CDD:410995"
     Site            order(50,55,69,73)
                     /site_type="other"
                     /note="reactive site [polypeptide binding]"
                     /db_xref="CDD:410995"
     CDS             1..100
                     /gene="LOC144115832"
                     /coded_by="XM_077650333.1:55..357"
                     /db_xref="GeneID:144115832"
ORIGIN      
        1 magpkaglsl ehnimrlhil alfvavlala faanaqgpgd cgpnetwkec vssscaektc
       61 trrfipdfct adcsfgcycg dnyyrnserh cvpveecpte
//
LOCUS       XP_077506460             100 aa            linear   INV 25-NOV-2025
DEFINITION  ixochymostatin-like [Amblyomma americanum].
ACCESSION   XP_077506460
VERSION     XP_077506460.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650334.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..100
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..100
                     /product="ixochymostatin-like"
                     /calculated_mol_wt=10780
     Region          41..97
                     /region_name="TIL"
                     /note="trypsin inhibitor-like cysteine rich domain;
                     cd19941"
                     /db_xref="CDD:410995"
     Site            order(50,55,69,73)
                     /site_type="other"
                     /note="reactive site [polypeptide binding]"
                     /db_xref="CDD:410995"
     CDS             1..100
                     /gene="LOC144115832"
                     /coded_by="XM_077650334.1:55..357"
                     /db_xref="GeneID:144115832"
ORIGIN      
        1 magpkaglsl ehnimrlhil alfvavlala faanaqgpgd cgpnetwkec vssscaektc
       61 trrfipdfct adcsfgcycg dnyyrnserh cvpveecpte
//
LOCUS       XP_077506461             124 aa            linear   INV 25-NOV-2025
DEFINITION  ixochymostatin-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077506461
VERSION     XP_077506461.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650335.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..124
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..124
                     /product="ixochymostatin-like isoform X1"
                     /calculated_mol_wt=13357
     Region          65..121
                     /region_name="TIL"
                     /note="trypsin inhibitor-like cysteine rich domain;
                     cd19941"
                     /db_xref="CDD:410995"
     Site            order(74,79,93,97)
                     /site_type="other"
                     /note="reactive site [polypeptide binding]"
                     /db_xref="CDD:410995"
     CDS             1..124
                     /gene="LOC144115833"
                     /coded_by="XM_077650335.1:387..761"
                     /db_xref="GeneID:144115833"
ORIGIN      
        1 mngtlvvvlc fvalglaada yftvsrkvip sagvvhmfqs vnihgqdere esdsvlrwpp
       61 tpqecaeget wkecvsgsca eatcerpvvg pactddcnyg cycadgfyrn qhkacvtrdk
      121 csqa
//
LOCUS       XP_077506462              94 aa            linear   INV 25-NOV-2025
DEFINITION  ixochymostatin-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077506462
VERSION     XP_077506462.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650336.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..94
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..94
                     /product="ixochymostatin-like isoform X2"
                     /calculated_mol_wt=10001
     Region          35..91
                     /region_name="TIL"
                     /note="trypsin inhibitor-like cysteine rich domain;
                     cd19941"
                     /db_xref="CDD:410995"
     Site            order(44,49,63,67)
                     /site_type="other"
                     /note="reactive site [polypeptide binding]"
                     /db_xref="CDD:410995"
     CDS             1..94
                     /gene="LOC144115833"
                     /coded_by="XM_077650336.1:318..602"
                     /db_xref="GeneID:144115833"
ORIGIN      
        1 mngtlvvvlc fvalglaade esdsvlrwpp tpqecaeget wkecvsgsca eatcerpvvg
       61 pactddcnyg cycadgfyrn qhkacvtrdk csqa
//
LOCUS       XP_077506464             134 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115835 [Amblyomma americanum].
ACCESSION   XP_077506464
VERSION     XP_077506464.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650338.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..134
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..134
                     /product="uncharacterized protein LOC144115835"
                     /calculated_mol_wt=14113
     CDS             1..134
                     /gene="LOC144115835"
                     /coded_by="XM_077650338.1:1..405"
                     /db_xref="GeneID:144115835"
ORIGIN      
        1 mlppplpdng pfnisfipah pmkslafgrs tpsttasspa rnlccglsvl gfsghennar
       61 smtpdfrfap mqglqctipp amasrqaask mtlaiidcat aifhtqhgll vsaqlrhchv
      121 ataseltgql kmpm
//
LOCUS       XP_077506465             553 aa            linear   INV 25-NOV-2025
DEFINITION  glutathione hydrolase-like YwrD proenzyme [Amblyomma americanum].
ACCESSION   XP_077506465
VERSION     XP_077506465.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650339.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..553
                     /product="glutathione hydrolase-like YwrD proenzyme"
                     /calculated_mol_wt=58711
     Region          19..513
                     /region_name="Ggt"
                     /note="Gamma-glutamyltranspeptidase [Amino acid transport
                     and metabolism]; COG0405"
                     /db_xref="CDD:440174"
     CDS             1..553
                     /gene="LOC144115837"
                     /coded_by="XM_077650339.1:67..1728"
                     /db_xref="GeneID:144115837"
ORIGIN      
        1 mdglftsrrs pvmslgggas cddplataaa vellnhggna adaavaaaaa lqvlkpyatg
       61 iggdcfalfy daetravrci dgsgpspasl trelfaagad sgmeatvpga vkgwfdtlrh
      121 cgsgrvgmre vlapavrlgr ggfpvgvrna sqwstyevkl rglvggrffl pvpwagqvvy
      181 napladller lgrdgagafy egpvaeavat avqraggvlt pddlrrhlea scdglvepvs
      241 ttyrgtrvht tplpshgsvl lealnilerf elrqldsaay ehlvvealrl aledglgwva
      301 hggpaqdgrm tskehsktrl vdperardcr gmvlptspeq shtvflvtad grgnacafin
      361 snfygfgcsv veehgfavhc rgrgfnalar hpncagpfkk pyhtlmpvlv tdtasgdwla
      421 algtmggytq pqvdlqifla maergldpqa aldaprlyig vgrtlrvddp lfleegfped
      481 vaanlerrgh riescrarrl dpaptsiahi vtrgswwnst aksdvkesgp vlwfgcdprc
      541 dgtaaaatpy dlp
//
LOCUS       XP_077506466             333 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115839 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506466
VERSION     XP_077506466.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650340.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..333
                     /product="uncharacterized protein LOC144115839 isoform X1"
                     /calculated_mol_wt=36900
     Region          62..145
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..333
                     /gene="LOC144115839"
                     /coded_by="XM_077650340.1:1506..2507"
                     /db_xref="GeneID:144115839"
ORIGIN      
        1 maaplriile gvereifeav ddtgapivhr nqrlyatadn vqvwlnapaa pvphtiepee
       61 lwprakvlfl iqeykhfksd pskrlktkrq pwenladlvn skfgchlths qienkwrnle
      121 rkyktvrqhn sqsgadrrtc efeeelaeif ekahsinppy llgpglvrsv qdsqaepaas
      181 tqpvpsrvta taspipdssq pstsrgstta gdsqppspsa epsplqqqpg parkrprtdt
      241 sravlesava hlevaeanrq qrhrermale grkvaaverr taalervaaa lerrelvsvc
      301 spihapahsp thspihspir spvrspsppy yna
//
LOCUS       XP_077506467             333 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115839 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506467
VERSION     XP_077506467.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650341.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..333
                     /product="uncharacterized protein LOC144115839 isoform X1"
                     /calculated_mol_wt=36900
     Region          62..145
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..333
                     /gene="LOC144115839"
                     /coded_by="XM_077650341.1:1427..2428"
                     /db_xref="GeneID:144115839"
ORIGIN      
        1 maaplriile gvereifeav ddtgapivhr nqrlyatadn vqvwlnapaa pvphtiepee
       61 lwprakvlfl iqeykhfksd pskrlktkrq pwenladlvn skfgchlths qienkwrnle
      121 rkyktvrqhn sqsgadrrtc efeeelaeif ekahsinppy llgpglvrsv qdsqaepaas
      181 tqpvpsrvta taspipdssq pstsrgstta gdsqppspsa epsplqqqpg parkrprtdt
      241 sravlesava hlevaeanrq qrhrermale grkvaaverr taalervaaa lerrelvsvc
      301 spihapahsp thspihspir spvrspsppy yna
//
LOCUS       XP_077506468             333 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115839 isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506468
VERSION     XP_077506468.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650342.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..333
                     /product="uncharacterized protein LOC144115839 isoform X1"
                     /calculated_mol_wt=36900
     Region          62..145
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..333
                     /gene="LOC144115839"
                     /coded_by="XM_077650342.1:1419..2420"
                     /db_xref="GeneID:144115839"
ORIGIN      
        1 maaplriile gvereifeav ddtgapivhr nqrlyatadn vqvwlnapaa pvphtiepee
       61 lwprakvlfl iqeykhfksd pskrlktkrq pwenladlvn skfgchlths qienkwrnle
      121 rkyktvrqhn sqsgadrrtc efeeelaeif ekahsinppy llgpglvrsv qdsqaepaas
      181 tqpvpsrvta taspipdssq pstsrgstta gdsqppspsa epsplqqqpg parkrprtdt
      241 sravlesava hlevaeanrq qrhrermale grkvaaverr taalervaaa lerrelvsvc
      301 spihapahsp thspihspir spvrspsppy yna
//
LOCUS       XP_077506469             332 aa            linear   INV 25-NOV-2025
DEFINITION  uncharacterized protein LOC144115839 isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506469
VERSION     XP_077506469.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650343.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..332
                     /product="uncharacterized protein LOC144115839 isoform X2"
                     /calculated_mol_wt=36813
     Region          62..145
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..332
                     /gene="LOC144115839"
                     /coded_by="XM_077650343.1:1506..2504"
                     /db_xref="GeneID:144115839"
ORIGIN      
        1 maaplriile gvereifeav ddtgapivhr nqrlyatadn vqvwlnapaa pvphtiepee
       61 lwprakvlfl iqeykhfksd pskrlktkrq pwenladlvn skfgchlths qienkwrnle
      121 rkyktvrqhn sqsgadrrtc efeeelaeif ekahsinppy llgpglvrsv qdqaepaast
      181 qpvpsrvtat aspipdssqp stsrgsttag dsqppspsae psplqqqpgp arkrprtdts
      241 ravlesavah levaeanrqq rhrermaleg rkvaaverrt aalervaaal errelvsvcs
      301 pihapahspt hspihspirs pvrspsppyy na
//
LOCUS       XP_077506471             655 aa            linear   INV 25-NOV-2025
DEFINITION  LOW QUALITY PROTEIN: topoisomerase 3-alpha [Amblyomma americanum].
ACCESSION   XP_077506471
VERSION     XP_077506471.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650345.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            internal stop codons :: corrected 1 genomic stop codon
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..655
                     /product="LOW QUALITY PROTEIN: topoisomerase 3-alpha"
                     /calculated_mol_wt=74405
     Region          81..>647
                     /region_name="PRK07219"
                     /note="DNA topoisomerase I; Validated"
                     /db_xref="CDD:235971"
     CDS             1..655
                     /gene="Top3alpha"
                     /coded_by="XM_077650345.1:106..2073"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: substituted 1 base at 1
                     genomic stop codon"
                     /db_xref="GeneID:144115841"
ORIGIN      
        1 mcwpcrgasr acsfgdrcil vnilsapsvr gscsalmltc lrsclrrris iyvdaaahys
       61 ygliltrrtn csknpdpkmr vlnvaeknda akniaellsq grynrregls nfnkiyefdy
      121 risnstcrmi mtsvsghlln ldfvndyrkw hscdpselfd apvaeictdq kyelikqtle
      181 reirycdtli iwtdcdrege nigfeiirvc rkvkptvrvy rarfseitpa avsravanlt
      241 npdprlnaav evrreldlri gaaftrlqtl rlkrefrqel gdrlvsygsc qfptlgfvve
      301 ryrailnfvp epfwkiemsh tkdsikaefs wkrhrlfdkh vcsvlyxrcv hgnppqarvv
      361 evrskpkskw rpqpmdtvel eklasrklri takdtmqiae klytdgfisy prtettkfpp
      421 dlnlrplvea qtqdprwggf asrllqdglt prqgnktdqa hppihpvkga shlegdakrv
      481 yelivrhfla clskdaegke ttvevdlnde rfvvtglvif arnylevyiy dkwnakeips
      541 yregecfvpd svklaegett pppllreadl ialmekhgig tdathaehie tvknrlyval
      601 tgdgylvpge lgmglvegyd smglemskph lraeleadlk kicegtknak dkkag
//
LOCUS       XP_077506472             305 aa            linear   INV 25-NOV-2025
DEFINITION  caspase-1-like isoform X1 [Amblyomma americanum].
ACCESSION   XP_077506472
VERSION     XP_077506472.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650346.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..305
                     /product="caspase-1-like isoform X1"
                     /calculated_mol_wt=34204
     Region          61..301
                     /region_name="CASc"
                     /note="Caspase, interleukin-1 beta converting enzyme (ICE)
                     homologues; Cysteine-dependent aspartate-directed
                     proteases that mediate programmed cell death (apoptosis).
                     Caspases are synthesized as inactive zymogens and
                     activated by proteolysis of the peptide...; cd00032"
                     /db_xref="CDD:237997"
     Site            order(88,146,185,192,229..234,238..239)
                     /site_type="other"
                     /note="substrate pocket [chemical binding]"
                     /db_xref="CDD:237997"
     Site            order(145,187)
                     /site_type="active"
                     /db_xref="CDD:237997"
     Site            order(193,214..215,222,225,228,251,259,265,286,291..292,
                     294,297..298)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:237997"
     CDS             1..305
                     /gene="LOC144115842"
                     /coded_by="XM_077650346.1:299..1216"
                     /db_xref="GeneID:144115842"
ORIGIN      
        1 manvsgdslq gndrnansns eadarsifgg lssgccmprm ftkrpathyp vlptsleaee
       61 ynmvhpnrgk cvifnnryfe phtklserrg tdldadnlyf rfrelgfepv ihanksrkdm
      121 leelerlgrq dysnddcfvc cilthgdrdv lyatdgkfpv dnvmdpfrgd vcptllgkpk
      181 lffiqacrgd rldagtpavl dtsdspgqiy ripthadfla aystvpgfys wrnttqgswf
      241 vqglcsvlqe rarsadllsm ltvvcrrval yyescvpgdp amdrkkqvps itstltrlvy
      301 fgrrs
//
LOCUS       XP_077506473             296 aa            linear   INV 25-NOV-2025
DEFINITION  caspase-1-like isoform X2 [Amblyomma americanum].
ACCESSION   XP_077506473
VERSION     XP_077506473.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650347.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..296
                     /product="caspase-1-like isoform X2"
                     /calculated_mol_wt=33269
     Region          52..292
                     /region_name="CASc"
                     /note="Caspase, interleukin-1 beta converting enzyme (ICE)
                     homologues; Cysteine-dependent aspartate-directed
                     proteases that mediate programmed cell death (apoptosis).
                     Caspases are synthesized as inactive zymogens and
                     activated by proteolysis of the peptide...; cd00032"
                     /db_xref="CDD:237997"
     Site            order(79,137,176,183,220..225,229..230)
                     /site_type="other"
                     /note="substrate pocket [chemical binding]"
                     /db_xref="CDD:237997"
     Site            order(136,178)
                     /site_type="active"
                     /db_xref="CDD:237997"
     Site            order(184,205..206,213,216,219,242,250,256,277,282..283,
                     285,288..289)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:237997"
     CDS             1..296
                     /gene="LOC144115842"
                     /coded_by="XM_077650347.1:284..1174"
                     /db_xref="GeneID:144115842"
ORIGIN      
        1 manvsgdslq gndrnansns eadarsifgg mftkrpathy pvlptsleae eynmvhpnrg
       61 kcvifnnryf ephtklserr gtdldadnly frfrelgfep vihanksrkd mleelerlgr
      121 qdysnddcfv ccilthgdrd vlyatdgkfp vdnvmdpfrg dvcptllgkp klffiqacrg
      181 drldagtpav ldtsdspgqi yripthadfl aaystvpgfy swrnttqgsw fvqglcsvlq
      241 erarsadlls mltvvcrrva lyyescvpgd pamdrkkqvp sitstltrlv yfgrrs
//
LOCUS       XP_077506474             332 aa            linear   INV 25-NOV-2025
DEFINITION  tRNA methyltransferase 10 homolog A-like isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506474
VERSION     XP_077506474.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650348.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..332
                     /product="tRNA methyltransferase 10 homolog A-like isoform
                     X1"
                     /calculated_mol_wt=37830
     Region          118..285
                     /region_name="SPOUT_MTase"
                     /note="SPOUT superfamily of SAM-dependent RNA
                     methyltransferases; cl38912"
                     /db_xref="CDD:476814"
     Site            order(201..203,220..223,245..247,261,263)
                     /site_type="other"
                     /note="SAM binding site [chemical binding]"
                     /db_xref="CDD:349952"
     CDS             1..332
                     /gene="LOC144115844"
                     /coded_by="XM_077650348.1:134..1132"
                     /db_xref="GeneID:144115844"
ORIGIN      
        1 metrmdehat pvndsvncev essaiengvd snspkkefnm mtiveleavl ndphstvskr
       61 yrkrvlrriq wlkrrperra lekekkkrkr eeckgangea asykrpkftk meesscrirv
      121 aidlsldghm tdkekvkcrk qiqrcyslnr rspapvqlyl tgmagsfkni ssymhgfdkw
      181 dvhtsdadhl scfgkenvvy lssesenelq eleegkayii gglvdhnhsk glchrlaeeq
      241 gvkharlpig nclvmktrqv ltidqgllqi aqdgvdwkpl geafcaavdf awltarpeci
      301 hmrhtktail ylllhslsfr fsrqaglrdv vs
//
LOCUS       XP_077506475             316 aa            linear   INV 25-NOV-2025
DEFINITION  tRNA methyltransferase 10 homolog A-like isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506475
VERSION     XP_077506475.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650349.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..316
                     /product="tRNA methyltransferase 10 homolog A-like isoform
                     X2"
                     /calculated_mol_wt=35757
     Region          118..291
                     /region_name="Trm10euk_A"
                     /note="eukaryotic tRNA m1G9 methyltransferase Trm10
                     homolog A; cd18101"
                     /db_xref="CDD:349974"
     Site            order(201..203,220..221,223,225,246..247,249,260..261,263,
                     266)
                     /site_type="other"
                     /note="SAM binding site [chemical binding]"
                     /db_xref="CDD:349974"
     CDS             1..316
                     /gene="LOC144115844"
                     /coded_by="XM_077650349.1:168..1118"
                     /db_xref="GeneID:144115844"
ORIGIN      
        1 metrmdehat pvndsvncev essaiengvd snspkkefnm mtiveleavl ndphstvskr
       61 yrkrvlrriq wlkrrperra lekekkkrkr eeckgangea asykrpkftk meesscrirv
      121 aidlsldghm tdkekvkcrk qiqrcyslnr rspapvqlyl tgmagsfkni ssymhgfdkw
      181 dvhtsdadhl scfgkenvvy lssesenelq eleegkayii gglvdhnhsk glchrlaeeq
      241 gvkharlpig nclvmktrqv ltidqvfail lkvtegkswt eallaaipqr kgakalahes
      301 essslhnsdg deskdm
//
LOCUS       XP_077506476             361 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial calcium uniporter isoform X1 [Amblyomma americanum].
ACCESSION   XP_077506476
VERSION     XP_077506476.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650350.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..361
                     /product="mitochondrial calcium uniporter isoform X1"
                     /calculated_mol_wt=41746
     Region          120..322
                     /region_name="MCU"
                     /note="Mitochondrial calcium uniporter; pfam04678"
                     /db_xref="CDD:461389"
     CDS             1..361
                     /gene="MCU"
                     /coded_by="XM_077650350.1:49..1134"
                     /db_xref="GeneID:144115845"
ORIGIN      
        1 mafwrvtvsn cglllshlpr rnrtksrkyl qlclcpfsna awnhkcrnte wslqpwhclp
       61 acgihssslp apitgevtvv ykdglpeitv plpsrrdrcr ftlrplgstv gdlmrsicve
      121 drgvdhvsvt sadgvriast ssiesllren fvltlnnqvy lveppsierl tseetqtlsd
      181 lkmlvarlye sltvnehqle qerklldrlh rvkeqlgple qkknelmtls qrrttllswl
      241 glglmgvqfg ilarltwwey swdimepvty fitygttmam yayfvvtrqd yvlpevrdrq
      301 flisfykfar rqgmdvsryn dlrqelaeie eelrrlrdpl qkhlpvrelg spcghdeert
      361 s
//
LOCUS       XP_077506477             304 aa            linear   INV 25-NOV-2025
DEFINITION  mitochondrial calcium uniporter isoform X2 [Amblyomma americanum].
ACCESSION   XP_077506477
VERSION     XP_077506477.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650351.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..304
                     /product="mitochondrial calcium uniporter isoform X2"
                     /calculated_mol_wt=35464
     Region          76..265
                     /region_name="MCU"
                     /note="Mitochondrial calcium uniporter; pfam04678"
                     /db_xref="CDD:461389"
     CDS             1..304
                     /gene="MCU"
                     /coded_by="XM_077650351.1:49..963"
                     /db_xref="GeneID:144115845"
ORIGIN      
        1 mafwrvtvsn cglllshlpr rnrtksrkyl qlclcpfsna awnhkcrnte wslqpwhclp
       61 acgihssslp apitgdgvri astssiesll renfvltlnn qvylveppsi erltseetqt
      121 lsdlkmlvar lyesltvneh qleqerklld rlhrvkeqlg pleqkknelm tlsqrrttll
      181 swlglglmgv qfgilarltw weyswdimep vtyfitygtt mamyayfvvt rqdyvlpevr
      241 drqflisfyk farrqgmdvs ryndlrqela eieeelrrlr dplqkhlpvr elgspcghde
      301 erts
//
LOCUS       XP_077506478            2816 aa            linear   INV 25-NOV-2025
DEFINITION  multiple ankyrin repeats single KH domain isoform X1 [Amblyomma
            americanum].
ACCESSION   XP_077506478
VERSION     XP_077506478.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650352.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2816
                     /product="multiple ankyrin repeats single KH domain
                     isoform X1"
                     /calculated_mol_wt=291752
     Region          201..466
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          201..227
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          229..260
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          265..293
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(268..269,272..274,276..277,281,284,293,295,297,
                     301..302,305..307,309..310,314,317,326,328,330,334..335,
                     338..340,342..343,347,350)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          295..326
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          328..356
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          362..392
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          376..680
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(397,401..402,405..407,409..410,414,417,426,428,430,
                     434..435,438..440,442..443,447,450,459,461,463,467..468,
                     471..473,475..476,480,483)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          397..426
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          428..459
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          461..491
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          494..526
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          528..556
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(531..532,535..537,539..540,544,547,556,558,560,
                     564..565,568..570,572..573,577,580,589,591,593,597..598,
                     601..603,605..606,610,613)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          558..589
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          591..620
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          625..655
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1149..1174
                     /region_name="ANK"
                     /note="ankyrin repeats; smart00248"
                     /db_xref="CDD:197603"
     Region          1151..1180
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1164..1451
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          1182..1214
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1218,1222..1223,1226..1228,1230..1231,1235,1238,
                     1247,1249,1251,1255..1256,1259..1261,1263..1264,1268,1271,
                     1280,1284,1286,1290..1291,1294..1296,1298..1299,1303,1306)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1218..1247
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1249..1280
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1284..1314
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1318..1348
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1351..1384
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1386..1417
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1419..1450
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1933..2002
                     /region_name="KH-I_MASK"
                     /note="type I K homology (KH) RNA-binding domain found in
                     Mask family proteins; cd22404"
                     /db_xref="CDD:411832"
     Site            order(1942,1944..1946,1948..1952,1955..1956,1959..1960,
                     1965..1968)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411832"
     CDS             1..2816
                     /gene="mask"
                     /coded_by="XM_077650352.1:143..8593"
                     /db_xref="GeneID:144115847"
ORIGIN      
        1 mqnvvhgnac davreepqev skgratgtsd nfetgqrqsl nslhsnfmad ltesdddeas
       61 eaesfildpe eleeervldt skfllssseg gggespelqv gtvdpdtqar lealleatgl
      121 sklslegkqf adpevlrrlt tsvscaldea aaaltrmrae hgggvphqqq qqvqqqpqqq
      181 qqqqqqqsst gvvaasssrt laeacsegdv ravrqlldeg rnvnevteeg esllslacas
      241 gycelaqlll amranvedrg lkdmtplmea ataghvdivr lliehgadvn aqtaqghtpl
      301 mfaclgghea aaralveaga nleehnengh tplmeaasag hvstarvlva agasinthsn
      361 efkesaltla cykghlemvr flleagadqe hktdemhtal measmdghve varllldsga
      421 qvnmptdsfe spltlaacgg hvelamllle rganieevnd egytplmeaa regheemval
      481 llsqgadina qteetqetal tlaccggfle vadfllkaga qlelgastpl meaaqeghle
      541 llrylinaga svnaktatgd taltyaceng htdvadlllq anadlehese ggrtplmkac
      601 raghlctvqf llgrgadpnr ptssndhtpl slacagghls vvelllahga dpshrlkdns
      661 tmlieaskgg htavvqllld ypnlgtaaaa aaaaaaaaaa avtstasatt aaapspavel
      721 cavsltagla pqvvpmteeq rvaalaaava aghpylpqva gvtprvsvaa asssaaapst
      781 daawppsast pstsgkrkas gdsssgplvs cgggtvvarg gadqlakpgq gqpslrpser
      841 leacissmmr saelnhpsre eqimqkqqil eelqrverel qgskdltpne dgahacawql
      901 htafarqqae ekahaqivls aqhhqhlava astedvveel nlrnaalsss svvvppstga
      961 aaaeitalpp sslpssstat pstllpaaak rhldkvkass gkqqlppaag aavtvpttkd
     1021 astsmvcppg gasllgsfga appqaeglmv aapartlhea lgeivtgpta agtqcgggsg
     1081 aqvsitaqqa aavcpdhhhh qhhhhhhhhg gsthpaassl tqqpptlafh paafpihaqv
     1141 dlnwqtesnh dtaltlacag ghedlvslll nrgahlehrd kkgftplmla ataghagvvd
     1201 illshgadle aqsertkdta lslacsggry evveillarg ankehrnvsd ytplslaasg
     1261 gyvniiklll qhgaeinsrt gsklgisplm laamnghvaa vrllldngsd inaqietnkn
     1321 taltlacfqg rqevvallvd rkanvehrak tgltplmeaa sggyvevgrv lldkgadvna
     1381 ppvpssrdta ltiaadkghy afvellikrg aaidvknkkg ssplwlacng gyldvvqllv
     1441 aaqadidsmd nrrvsclmaa frrghvkaak wlvkqvaqfp sdqemarfma tlgpdsrdll
     1501 rkchqctevi haakerqaae ankcasslle eldlersree srraaaarrr erkrrkkkek
     1561 qeqmrlakle aeggdkaasa sanngkdsdk dsksegdkqp tssqsedddd ddeeeeeedt
     1621 rtmaasagss rnsriaelde rlpnpggagg nhmatittgs sdrsggqpna pgrpkangas
     1681 ssstpskvrl keddlpplas hesssspsss ppkapssapa esknsrkkkq qaaacstaer
     1741 tadpasprln htvspspmas skggnsgskq gkaaqanqav lqqptstvtr spltvtqsst
     1801 vhrqaavvak rggevmvngl adgedfstvv kgtkhrgqgq ahhvatdtss ktstlgelal
     1861 dvmdscggpr spgngagrps pgvasspkks ssavlstscs tsmttcsaaa saatgtsksh
     1921 hrgeegwkev vrrskkvtvp staisrvigr cgcninaire asgahievek hkgqgertil
     1981 irgsaeatkq aqlliqglvq epdrdlsdim aqhgvvapss akapassldh rtttisssst
     2041 ttnhtgsssv lvsgssstnk lsrqgssakv vgvkaaapap slppfgqtrp aprpgipths
     2101 ggssgnstsv tsssappfas gsagwgnlas sslastkpaa taatpvtsas avvttapavs
     2161 tnpaalngtt ttsasytqav ispaksssra asadprpsss pssgggspsp tlgntgsmav
     2221 ssaatttsnt tmapsvstst sstntapais ipqtfspfnn vfsketqpsv wgqreaskpn
     2281 fasvaasgvc gvpsssggsv gsvsassepl vdaakapgyr gnlhvsppnf gpigsgprsa
     2341 pctppiggga ppsppsqpqp aaqpqaqefy enasapttav pqpvqatpqp sssrlvfgpp
     2401 hyaatapela pppqqqqqqq ppqqqqtiqs tlnpnapdfs srsaapvmlt apfpqvgtsh
     2461 hhqhhhpaat pshhgmrvpf apqeymhtvq ssshmshhhp hhhqgaagal ghpgvfplqm
     2521 mshlqqqysg lvapvgqmpk gadraplgvr ltdegspsav spqgglspgg glddrklprp
     2581 igteraqkkg ahtsypgiws ysnsaagvgs sgggssltpd lsamqawvqy qhatgpqgdg
     2641 lapllqhnrf aaaaeaapeq qtleqhtyhh kycpkqggst ynrdqrryac ssdqtrpgvp
     2701 trlaipnfnt vsqpqqpaln agglgscppf lngfpggatg amlggaaqlf snrvaaeaph
     2761 qeatpwsphk lmgsapgdsl hhhaghhphh pqqqpphhah vataklpwqs wthhqt
//
LOCUS       XP_077506479            2815 aa            linear   INV 25-NOV-2025
DEFINITION  multiple ankyrin repeats single KH domain isoform X2 [Amblyomma
            americanum].
ACCESSION   XP_077506479
VERSION     XP_077506479.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650353.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2815
                     /product="multiple ankyrin repeats single KH domain
                     isoform X2"
                     /calculated_mol_wt=291637
     Region          201..466
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          201..227
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          229..260
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          265..293
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(268..269,272..274,276..277,281,284,293,295,297,
                     301..302,305..307,309..310,314,317,326,328,330,334..335,
                     338..340,342..343,347,350)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          295..326
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          328..356
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          362..392
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          376..680
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(397,401..402,405..407,409..410,414,417,426,428,430,
                     434..435,438..440,442..443,447,450,459,461,463,467..468,
                     471..473,475..476,480,483)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          397..426
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          428..459
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          461..491
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          494..526
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          528..556
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(531..532,535..537,539..540,544,547,556,558,560,
                     564..565,568..570,572..573,577,580,589,591,593,597..598,
                     601..603,605..606,610,613)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          558..589
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          591..620
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          625..655
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1150..1175
                     /region_name="ANK"
                     /note="ankyrin repeats; smart00248"
                     /db_xref="CDD:197603"
     Region          1152..1181
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1165..1452
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          1183..1215
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1219,1223..1224,1227..1229,1231..1232,1236,1239,
                     1248,1250,1252,1256..1257,1260..1262,1264..1265,1269,1272,
                     1281,1285,1287,1291..1292,1295..1297,1299..1300,1304,1307)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1219..1248
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1250..1281
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1285..1315
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1319..1349
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1352..1385
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1387..1418
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1420..1451
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1934..2003
                     /region_name="KH-I_MASK"
                     /note="type I K homology (KH) RNA-binding domain found in
                     Mask family proteins; cd22404"
                     /db_xref="CDD:411832"
     Site            order(1943,1945..1947,1949..1953,1956..1957,1960..1961,
                     1966..1969)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411832"
     CDS             1..2815
                     /gene="mask"
                     /coded_by="XM_077650353.1:143..8590"
                     /db_xref="GeneID:144115847"
ORIGIN      
        1 mqnvvhgnac davreepqev skgratgtsd nfetgqrqsl nslhsnfmad ltesdddeas
       61 eaesfildpe eleeervldt skfllssseg gggespelqv gtvdpdtqar lealleatgl
      121 sklslegkqf adpevlrrlt tsvscaldea aaaltrmrae hgggvphqqq qqvqqqpqqq
      181 qqqqqqqsst gvvaasssrt laeacsegdv ravrqlldeg rnvnevteeg esllslacas
      241 gycelaqlll amranvedrg lkdmtplmea ataghvdivr lliehgadvn aqtaqghtpl
      301 mfaclgghea aaralveaga nleehnengh tplmeaasag hvstarvlva agasinthsn
      361 efkesaltla cykghlemvr flleagadqe hktdemhtal measmdghve varllldsga
      421 qvnmptdsfe spltlaacgg hvelamllle rganieevnd egytplmeaa regheemval
      481 llsqgadina qteetqetal tlaccggfle vadfllkaga qlelgastpl meaaqeghle
      541 llrylinaga svnaktatgd taltyaceng htdvadlllq anadlehese ggrtplmkac
      601 raghlctvqf llgrgadpnr ptssndhtpl slacagghls vvelllahga dpshrlkdns
      661 tmlieaskgg htavvqllld ypnlgtaaaa aaaaaaaaaa avtstasatt aaapspavel
      721 cavsltagla pqvvpmteeq rvaalaaava aghpylpqva gavtprvsva aasssaaaps
      781 tdaawppsas tpstsgkrka sgdsssgplv scgggtvvar ggadqlakpg qgqpslrpse
      841 rleacissmm rsaelnhpsr eeqimqkqqi leelqrvere lqgskdltpn edgahacawq
      901 lhtafarqqa eekahaqivl saqhhqhlav aastedvvee lnlrnaalss ssvvvppstg
      961 aaaaeitalp psslpsssta tpstllpaaa krhldkvkas sgkqqlppaa gaavtvpttk
     1021 dastsmvcpp ggasllgsfg aappqaeglm vaapartlhe algeivtgpt aagtqcgggs
     1081 gaqvsitaqq aaavcpdhhh hqhhhhhhhh ggsthpaass ltqqpptlaf hpaafpihaq
     1141 vdlnwqtesn hdtaltlaca gghedlvsll lnrgahlehr dkkgftplml aataghagvv
     1201 dillshgadl eaqsertkdt alslacsggr yevveillar gankehrnvs dytplslaas
     1261 ggyvniikll lqhgaeinsr tgsklgispl mlaamnghva avrllldngs dinaqietnk
     1321 ntaltlacfq grqevvallv drkanvehra ktgltplmea asggyvevgr vlldkgadvn
     1381 appvpssrdt altiaadkgh yafvellikr gaaidvknkk gssplwlacn ggyldvvqll
     1441 vaaqadidsm dnrrvsclma afrrghvkaa kwlvkqvaqf psdqemarfm atlgpdsrdl
     1501 lrkchqctev ihaakerqaa eankcassll eeldlersre esrraaaarr rerkrrkkke
     1561 kqeqmrlakl eaeggdkaas asanngkdsd kdsksegdkq ptssqseddd dddeeeeeed
     1621 trtmaasags srnsriaeld erlpnpggag gnhmatittg ssdrsggqpn apgrpkanga
     1681 sssstpskvr lkeddlppla shesssspss sppkapssap aesknsrkkk qqaaacstae
     1741 rtadpasprl nhtvspspma sskggnsgsk qgkaaqanqa vlqqptstvt rspltvtqss
     1801 tvhrqaavva krggevmvng ladgedfstv vkgtkhrgqg qahhvatdts sktstlgela
     1861 ldvmdscggp rspgngagrp spgvasspkk sssavlstsc stsmttcsaa asaatgtsks
     1921 hhrgeegwke vvrrskkvtv pstaisrvig rcgcninair easgahieve khkgqgerti
     1981 lirgsaeatk qaqlliqglv qepdrdlsdi maqhgvvaps sakapassld hrtttissss
     2041 tttnhtgsss vlvsgssstn klsrqgssak vvgvkaaapa pslppfgqtr paprpgipth
     2101 sggssgnsts vtsssappfa sgsagwgnla ssslastkpa ataatpvtsa savvttapav
     2161 stnpaalngt tttsasytqa vispaksssr aasadprpss spssgggsps ptlgntgsma
     2221 vssaatttsn ttmapsvsts tsstntapai sipqtfspfn nvfsketqps vwgqreaskp
     2281 nfasvaasgv cgvpsssggs vgsvsassep lvdaakapgy rgnlhvsppn fgpigsgprs
     2341 apctppiggg appsppsqpq paaqpqaqef yenasaptta vpqpvqatpq psssrlvfgp
     2401 phyaatapel apppqqqqqq qppqqqqtiq stlnpnapdf ssrsaapvml tapfpqvgts
     2461 hhhqhhhpaa tpshhgmrvp fapqeymhtv qssshmshhh phhhqgaaga lghpgvfplq
     2521 mmshlqqqys glvapvgqmp kgadraplgv rltdegspsa vspqgglspg gglddrklpr
     2581 pigteraqkk gahtsypgiw sysnsaagvg ssgggssltp dlsamqawvq yqhatgpqgd
     2641 glapllqhnr faaaaeaape qqtleqhtyh hkycpkqggs tynrdqrrya cssdqtrpgv
     2701 ptrlaipnfn tqpqqpalna gglgscppfl ngfpggatga mlggaaqlfs nrvaaeaphq
     2761 eatpwsphkl mgsapgdslh hhaghhphhp qqqpphhahv ataklpwqsw thhqt
//
LOCUS       XP_077506480            2802 aa            linear   INV 25-NOV-2025
DEFINITION  multiple ankyrin repeats single KH domain isoform X3 [Amblyomma
            americanum].
ACCESSION   XP_077506480
VERSION     XP_077506480.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650354.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2802
                     /product="multiple ankyrin repeats single KH domain
                     isoform X3"
                     /calculated_mol_wt=290145
     Region          201..466
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          201..227
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          229..260
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          265..293
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(268..269,272..274,276..277,281,284,293,295,297,
                     301..302,305..307,309..310,314,317,326,328,330,334..335,
                     338..340,342..343,347,350)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          295..326
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          328..356
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          362..392
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          376..680
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(397,401..402,405..407,409..410,414,417,426,428,430,
                     434..435,438..440,442..443,447,450,459,461,463,467..468,
                     471..473,475..476,480,483)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          397..426
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          428..459
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          461..491
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          494..526
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          528..556
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(531..532,535..537,539..540,544,547,556,558,560,
                     564..565,568..570,572..573,577,580,589,591,593,597..598,
                     601..603,605..606,610,613)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          558..589
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          591..620
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          625..655
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1135..1160
                     /region_name="ANK"
                     /note="ankyrin repeats; smart00248"
                     /db_xref="CDD:197603"
     Region          1137..1166
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1150..1437
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          1168..1200
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1204,1208..1209,1212..1214,1216..1217,1221,1224,
                     1233,1235,1237,1241..1242,1245..1247,1249..1250,1254,1257,
                     1266,1270,1272,1276..1277,1280..1282,1284..1285,1289,1292)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1204..1233
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1235..1266
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1270..1300
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1304..1334
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1337..1370
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1372..1403
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1405..1436
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1919..1988
                     /region_name="KH-I_MASK"
                     /note="type I K homology (KH) RNA-binding domain found in
                     Mask family proteins; cd22404"
                     /db_xref="CDD:411832"
     Site            order(1928,1930..1932,1934..1938,1941..1942,1945..1946,
                     1951..1954)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411832"
     CDS             1..2802
                     /gene="mask"
                     /coded_by="XM_077650354.1:143..8551"
                     /db_xref="GeneID:144115847"
ORIGIN      
        1 mqnvvhgnac davreepqev skgratgtsd nfetgqrqsl nslhsnfmad ltesdddeas
       61 eaesfildpe eleeervldt skfllssseg gggespelqv gtvdpdtqar lealleatgl
      121 sklslegkqf adpevlrrlt tsvscaldea aaaltrmrae hgggvphqqq qqvqqqpqqq
      181 qqqqqqqsst gvvaasssrt laeacsegdv ravrqlldeg rnvnevteeg esllslacas
      241 gycelaqlll amranvedrg lkdmtplmea ataghvdivr lliehgadvn aqtaqghtpl
      301 mfaclgghea aaralveaga nleehnengh tplmeaasag hvstarvlva agasinthsn
      361 efkesaltla cykghlemvr flleagadqe hktdemhtal measmdghve varllldsga
      421 qvnmptdsfe spltlaacgg hvelamllle rganieevnd egytplmeaa regheemval
      481 llsqgadina qteetqetal tlaccggfle vadfllkaga qlelgastpl meaaqeghle
      541 llrylinaga svnaktatgd taltyaceng htdvadlllq anadlehese ggrtplmkac
      601 raghlctvqf llgrgadpnr ptssndhtpl slacagghls vvelllahga dpshrlkdns
      661 tmlieaskgg htavvqllld ypnlgtaaaa aaaaaaaaaa avtstasatt aaapspavel
      721 cavsltagla pqvvpmteeq rvaalaaava aghpylpqva gavtprvsva aasssaaaps
      781 tdaawppsas tpstsgkrka sgdsssgplv scgggtvvar ggadqlakpg qgqpslrpse
      841 rleacissmm rsaelnhpsr eeqimqkqqi leelqrvere lqgskdltpn edgahacawq
      901 lhtafarqqa ehlavaaste dvveelnlrn aalssssvvv ppstgaaaae italppsslp
      961 ssstatpstl lpaaakrhld kvkassgkqq lppaagaavt vpttkdasts mvcppggasl
     1021 lgsfgaappq aeglmvaapa rtlhealgei vtgptaagtq cgggsgaqvs itaqqaaavc
     1081 pdhhhhqhhh hhhhhggsth paassltqqp ptlafhpaaf pihaqvdlnw qtesnhdtal
     1141 tlacagghed lvslllnrga hlehrdkkgf tplmlaatag hagvvdills hgadleaqse
     1201 rtkdtalsla csggryevve illarganke hrnvsdytpl slaasggyvn iiklllqhga
     1261 einsrtgskl gisplmlaam nghvaavrll ldngsdinaq ietnkntalt lacfqgrqev
     1321 vallvdrkan vehraktglt plmeaasggy vevgrvlldk gadvnappvp ssrdtaltia
     1381 adkghyafve llikrgaaid vknkkgsspl wlacnggyld vvqllvaaqa didsmdnrrv
     1441 sclmaafrrg hvkaakwlvk qvaqfpsdqe marfmatlgp dsrdllrkch qctevihaak
     1501 erqaaeankc asslleeldl ersreesrra aaarrrerkr rkkkekqeqm rlakleaegg
     1561 dkaasasann gkdsdkdsks egdkqptssq seddddddee eeeedtrtma asagssrnsr
     1621 iaelderlpn pggaggnhma tittgssdrs ggqpnapgrp kangasssst pskvrlkedd
     1681 lpplashess sspsssppka pssapaeskn srkkkqqaaa cstaertadp asprlnhtvs
     1741 pspmasskgg nsgskqgkaa qanqavlqqp tstvtrsplt vtqsstvhrq aavvakrgge
     1801 vmvngladge dfstvvkgtk hrgqgqahhv atdtssktst lgelaldvmd scggprspgn
     1861 gagrpspgva sspkksssav lstscstsmt tcsaaasaat gtskshhrge egwkevvrrs
     1921 kkvtvpstai srvigrcgcn inaireasga hievekhkgq gertilirgs aeatkqaqll
     1981 iqglvqepdr dlsdimaqhg vvapssakap assldhrttt isssstttnh tgsssvlvsg
     2041 ssstnklsrq gssakvvgvk aaapapslpp fgqtrpaprp gipthsggss gnstsvtsss
     2101 appfasgsag wgnlasssla stkpaataat pvtsasavvt tapavstnpa alngttttsa
     2161 sytqavispa ksssraasad prpssspssg ggspsptlgn tgsmavssaa tttsnttmap
     2221 svststsstn tapaisipqt fspfnnvfsk etqpsvwgqr easkpnfasv aasgvcgvps
     2281 ssggsvgsvs asseplvdaa kapgyrgnlh vsppnfgpig sgprsapctp pigggappsp
     2341 psqpqpaaqp qaqefyenas apttavpqpv qatpqpsssr lvfgpphyaa tapelapppq
     2401 qqqqqqppqq qqtiqstlnp napdfssrsa apvmltapfp qvgtshhhqh hhpaatpshh
     2461 gmrvpfapqe ymhtvqsssh mshhhphhhq gaagalghpg vfplqmmshl qqqysglvap
     2521 vgqmpkgadr aplgvrltde gspsavspqg glspgggldd rklprpigte raqkkgahts
     2581 ypgiwsysns aagvgssggg ssltpdlsam qawvqyqhat gpqgdglapl lqhnrfaaaa
     2641 eaapeqqtle qhtyhhkycp kqggstynrd qrryacssdq trpgvptrla ipnfntvsqp
     2701 qqpalnaggl gscppflngf pggatgamlg gaaqlfsnrv aaeaphqeat pwsphklmgs
     2761 apgdslhhha ghhphhpqqq pphhahvata klpwqswthh qt
//
LOCUS       XP_077506482            2790 aa            linear   INV 25-NOV-2025
DEFINITION  multiple ankyrin repeats single KH domain isoform X4 [Amblyomma
            americanum].
ACCESSION   XP_077506482
VERSION     XP_077506482.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650356.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2790
                     /product="multiple ankyrin repeats single KH domain
                     isoform X4"
                     /calculated_mol_wt=289060
     Region          174..439
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          174..200
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          202..233
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          238..266
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(241..242,245..247,249..250,254,257,266,268,270,
                     274..275,278..280,282..283,287,290,299,301,303,307..308,
                     311..313,315..316,320,323)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          268..299
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          301..329
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          335..365
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          349..653
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(370,374..375,378..380,382..383,387,390,399,401,403,
                     407..408,411..413,415..416,420,423,432,434,436,440..441,
                     444..446,448..449,453,456)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          370..399
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          401..432
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          434..464
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          467..499
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          501..529
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(504..505,508..510,512..513,517,520,529,531,533,
                     537..538,541..543,545..546,550,553,562,564,566,570..571,
                     574..576,578..579,583,586)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          531..562
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          564..593
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          598..628
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1123..1148
                     /region_name="ANK"
                     /note="ankyrin repeats; smart00248"
                     /db_xref="CDD:197603"
     Region          1125..1154
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1138..1425
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          1156..1188
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1192,1196..1197,1200..1202,1204..1205,1209,1212,
                     1221,1223,1225,1229..1230,1233..1235,1237..1238,1242,1245,
                     1254,1258,1260,1264..1265,1268..1270,1272..1273,1277,1280)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1192..1221
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1223..1254
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1258..1288
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1292..1322
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1325..1358
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1360..1391
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1393..1424
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1907..1976
                     /region_name="KH-I_MASK"
                     /note="type I K homology (KH) RNA-binding domain found in
                     Mask family proteins; cd22404"
                     /db_xref="CDD:411832"
     Site            order(1916,1918..1920,1922..1926,1929..1930,1933..1934,
                     1939..1942)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411832"
     CDS             1..2790
                     /gene="mask"
                     /coded_by="XM_077650356.1:16..8388"
                     /db_xref="GeneID:144115847"
ORIGIN      
        1 mprcqraqts sardglaprd fslcraregl plfwaesfil dpeeleeerv ldtskfllss
       61 seggggespe lqvgtvdpdt qarleallea tglsklsleg kqfadpevlr rlttsvscal
      121 deaaaaltrm raehgggvph qqqqqvqqqp qqqqqqqqqq sstgvvaass srtlaeacse
      181 gdvravrqll degrnvnevt eegesllsla casgycelaq lllamranve drglkdmtpl
      241 meaataghvd ivrlliehga dvnaqtaqgh tplmfaclgg heaaaralve aganleehne
      301 nghtplmeaa saghvstarv lvaagasint hsnefkesal tlacykghle mvrflleaga
      361 dqehktdemh talmeasmdg hvevarllld sgaqvnmptd sfespltlaa cgghvelaml
      421 llerganiee vndegytplm eaaregheem valllsqgad inaqteetqe taltlaccgg
      481 flevadfllk agaqlelgas tplmeaaqeg hlellrylin agasvnakta tgdtaltyac
      541 enghtdvadl llqanadleh eseggrtplm kacraghlct vqfllgrgad pnrptssndh
      601 tplslacagg hlsvvellla hgadpshrlk dnstmlieas kgghtavvql lldypnlgta
      661 aaaaaaaaaa aaaavtstas attaaapspa velcavslta glapqvvpmt eeqrvaalaa
      721 avaaghpylp qvagavtprv svaaasssaa apstdaawpp sastpstsgk rkasgdsssg
      781 plvscgggtv varggadqla kpgqgqpslr pserleacis smmrsaelnh psreeqimqk
      841 qqileelqrv erelqgskdl tpnedgahac awqlhtafar qqaeekahaq ivlsaqhhqh
      901 lavaastedv veelnlrnaa lssssvvvpp stgaaaaeit alppsslpss statpstllp
      961 aaakrhldkv kassgkqqlp paagaavtvp ttkdastsmv cppggasllg sfgaappqae
     1021 glmvaapart lhealgeivt gptaagtqcg ggsgaqvsit aqqaaavcpd hhhhqhhhhh
     1081 hhhggsthpa assltqqppt lafhpaafpi haqvdlnwqt esnhdtaltl acagghedlv
     1141 slllnrgahl ehrdkkgftp lmlaatagha gvvdillshg adleaqsert kdtalslacs
     1201 ggryevveil largankehr nvsdytplsl aasggyvnii klllqhgaei nsrtgsklgi
     1261 splmlaamng hvaavrllld ngsdinaqie tnkntaltla cfqgrqevva llvdrkanve
     1321 hraktgltpl meaasggyve vgrvlldkga dvnappvpss rdtaltiaad kghyafvell
     1381 ikrgaaidvk nkkgssplwl acnggyldvv qllvaaqadi dsmdnrrvsc lmaafrrghv
     1441 kaakwlvkqv aqfpsdqema rfmatlgpds rdllrkchqc tevihaaker qaaeankcas
     1501 slleeldler sreesrraaa arrrerkrrk kkekqeqmrl akleaeggdk aasasanngk
     1561 dsdkdskseg dkqptssqse ddddddeeee eedtrtmaas agssrnsria elderlpnpg
     1621 gaggnhmati ttgssdrsgg qpnapgrpka ngasssstps kvrlkeddlp plashessss
     1681 psssppkaps sapaesknsr kkkqqaaacs taertadpas prlnhtvsps pmasskggns
     1741 gskqgkaaqa nqavlqqpts tvtrspltvt qsstvhrqaa vvakrggevm vngladgedf
     1801 stvvkgtkhr gqgqahhvat dtssktstlg elaldvmdsc ggprspgnga grpspgvass
     1861 pkksssavls tscstsmttc saaasaatgt skshhrgeeg wkevvrrskk vtvpstaisr
     1921 vigrcgcnin aireasgahi evekhkgqge rtilirgsae atkqaqlliq glvqepdrdl
     1981 sdimaqhgvv apssakapas sldhrtttis ssstttnhtg sssvlvsgss stnklsrqgs
     2041 sakvvgvkaa apapslppfg qtrpaprpgi pthsggssgn stsvtsssap pfasgsagwg
     2101 nlassslast kpaataatpv tsasavvtta pavstnpaal ngttttsasy tqavispaks
     2161 ssraasadpr pssspssggg spsptlgntg smavssaatt tsnttmapsv ststsstnta
     2221 paisipqtfs pfnnvfsket qpsvwgqrea skpnfasvaa sgvcgvpsss ggsvgsvsas
     2281 seplvdaaka pgyrgnlhvs ppnfgpigsg prsapctppi gggappspps qpqpaaqpqa
     2341 qefyenasap ttavpqpvqa tpqpsssrlv fgpphyaata pelapppqqq qqqqppqqqq
     2401 tiqstlnpna pdfssrsaap vmltapfpqv gtshhhqhhh paatpshhgm rvpfapqeym
     2461 htvqssshms hhhphhhqga agalghpgvf plqmmshlqq qysglvapvg qmpkgadrap
     2521 lgvrltdegs psavspqggl spggglddrk lprpigtera qkkgahtsyp giwsysnsaa
     2581 gvgssgggss ltpdlsamqa wvqyqhatgp qgdglapllq hnrfaaaaea apeqqtleqh
     2641 tyhhkycpkq ggstynrdqr ryacssdqtr pgvptrlaip nfntvsqpqq palnagglgs
     2701 cppflngfpg gatgamlgga aqlfsnrvaa eaphqeatpw sphklmgsap gdslhhhagh
     2761 hphhpqqqpp hhahvatakl pwqswthhqt
//
LOCUS       XP_077506483            2786 aa            linear   INV 25-NOV-2025
DEFINITION  multiple ankyrin repeats single KH domain isoform X5 [Amblyomma
            americanum].
ACCESSION   XP_077506483
VERSION     XP_077506483.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650357.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2786
                     /product="multiple ankyrin repeats single KH domain
                     isoform X5"
                     /calculated_mol_wt=288502
     Region          201..466
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          201..227
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          229..260
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          265..293
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(268..269,272..274,276..277,281,284,293,295,297,
                     301..302,305..307,309..310,314,317,326,328,330,334..335,
                     338..340,342..343,347,350)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          295..326
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          328..356
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          362..392
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          376..680
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(397,401..402,405..407,409..410,414,417,426,428,430,
                     434..435,438..440,442..443,447,450,459,461,463,467..468,
                     471..473,475..476,480,483)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          397..426
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          428..459
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          461..491
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          494..526
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          528..556
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(531..532,535..537,539..540,544,547,556,558,560,
                     564..565,568..570,572..573,577,580,589,591,593,597..598,
                     601..603,605..606,610,613)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          558..589
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          591..620
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          625..655
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1121..1146
                     /region_name="ANK"
                     /note="ankyrin repeats; smart00248"
                     /db_xref="CDD:197603"
     Region          1123..1152
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1136..1423
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          1154..1186
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1190,1194..1195,1198..1200,1202..1203,1207,1210,
                     1219,1221,1223,1227..1228,1231..1233,1235..1236,1240,1243,
                     1252,1256,1258,1262..1263,1266..1268,1270..1271,1275,1278)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1190..1219
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1221..1252
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1256..1286
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1290..1320
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1323..1356
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1358..1389
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1391..1422
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1905..1974
                     /region_name="KH-I_MASK"
                     /note="type I K homology (KH) RNA-binding domain found in
                     Mask family proteins; cd22404"
                     /db_xref="CDD:411832"
     Site            order(1914,1916..1918,1920..1924,1927..1928,1931..1932,
                     1937..1940)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411832"
     CDS             1..2786
                     /gene="mask"
                     /coded_by="XM_077650357.1:143..8503"
                     /db_xref="GeneID:144115847"
ORIGIN      
        1 mqnvvhgnac davreepqev skgratgtsd nfetgqrqsl nslhsnfmad ltesdddeas
       61 eaesfildpe eleeervldt skfllssseg gggespelqv gtvdpdtqar lealleatgl
      121 sklslegkqf adpevlrrlt tsvscaldea aaaltrmrae hgggvphqqq qqvqqqpqqq
      181 qqqqqqqsst gvvaasssrt laeacsegdv ravrqlldeg rnvnevteeg esllslacas
      241 gycelaqlll amranvedrg lkdmtplmea ataghvdivr lliehgadvn aqtaqghtpl
      301 mfaclgghea aaralveaga nleehnengh tplmeaasag hvstarvlva agasinthsn
      361 efkesaltla cykghlemvr flleagadqe hktdemhtal measmdghve varllldsga
      421 qvnmptdsfe spltlaacgg hvelamllle rganieevnd egytplmeaa regheemval
      481 llsqgadina qteetqetal tlaccggfle vadfllkaga qlelgastpl meaaqeghle
      541 llrylinaga svnaktatgd taltyaceng htdvadlllq anadlehese ggrtplmkac
      601 raghlctvqf llgrgadpnr ptssndhtpl slacagghls vvelllahga dpshrlkdns
      661 tmlieaskgg htavvqllld ypnlgtaaaa aaaaaaaaaa avtstasatt aaapspavel
      721 cavsltagla pqvvpmteeq rvaalaaava aghpylpqva gavtprvsva aasssaaaps
      781 tdaawppsas tpstsgkrka sgdsssgplv scgggtvvar ggadqlakpg qgqpslrpse
      841 rleacissmm rsaelnhpsr eeqimqkqqi leelqrvere lqekahaqiv lsaqhhqhla
      901 vaastedvve elnlrnaals sssvvvppst gaaaaeital ppsslpssst atpstllpaa
      961 akrhldkvka ssgkqqlppa agaavtvptt kdastsmvcp pggasllgsf gaappqaegl
     1021 mvaapartlh ealgeivtgp taagtqcggg sgaqvsitaq qaaavcpdhh hhqhhhhhhh
     1081 hggsthpaas sltqqpptla fhpaafpiha qvdlnwqtes nhdtaltlac agghedlvsl
     1141 llnrgahleh rdkkgftplm laataghagv vdillshgad leaqsertkd talslacsgg
     1201 ryevveilla rgankehrnv sdytplslaa sggyvniikl llqhgaeins rtgsklgisp
     1261 lmlaamnghv aavrllldng sdinaqietn kntaltlacf qgrqevvall vdrkanvehr
     1321 aktgltplme aasggyvevg rvlldkgadv nappvpssrd taltiaadkg hyafvellik
     1381 rgaaidvknk kgssplwlac nggyldvvql lvaaqadids mdnrrvsclm aafrrghvka
     1441 akwlvkqvaq fpsdqemarf matlgpdsrd llrkchqcte vihaakerqa aeankcassl
     1501 leeldlersr eesrraaaar rrerkrrkkk ekqeqmrlak leaeggdkaa sasanngkds
     1561 dkdsksegdk qptssqsedd ddddeeeeee dtrtmaasag ssrnsriael derlpnpgga
     1621 ggnhmatitt gssdrsggqp napgrpkang asssstpskv rlkeddlppl ashessssps
     1681 ssppkapssa paesknsrkk kqqaaacsta ertadpaspr lnhtvspspm asskggnsgs
     1741 kqgkaaqanq avlqqptstv trspltvtqs stvhrqaavv akrggevmvn gladgedfst
     1801 vvkgtkhrgq gqahhvatdt ssktstlgel aldvmdscgg prspgngagr pspgvasspk
     1861 ksssavlsts cstsmttcsa aasaatgtsk shhrgeegwk evvrrskkvt vpstaisrvi
     1921 grcgcninai reasgahiev ekhkgqgert ilirgsaeat kqaqlliqgl vqepdrdlsd
     1981 imaqhgvvap ssakapassl dhrtttisss stttnhtgss svlvsgssst nklsrqgssa
     2041 kvvgvkaaap apslppfgqt rpaprpgipt hsggssgnst svtsssappf asgsagwgnl
     2101 assslastkp aataatpvts asavvttapa vstnpaalng ttttsasytq avispaksss
     2161 raasadprps sspssgggsp sptlgntgsm avssaattts nttmapsvst stsstntapa
     2221 isipqtfspf nnvfsketqp svwgqreask pnfasvaasg vcgvpsssgg svgsvsasse
     2281 plvdaakapg yrgnlhvspp nfgpigsgpr sapctppigg gappsppsqp qpaaqpqaqe
     2341 fyenasaptt avpqpvqatp qpsssrlvfg pphyaatape lapppqqqqq qqppqqqqti
     2401 qstlnpnapd fssrsaapvm ltapfpqvgt shhhqhhhpa atpshhgmrv pfapqeymht
     2461 vqssshmshh hphhhqgaag alghpgvfpl qmmshlqqqy sglvapvgqm pkgadraplg
     2521 vrltdegsps avspqgglsp ggglddrklp rpigteraqk kgahtsypgi wsysnsaagv
     2581 gssgggsslt pdlsamqawv qyqhatgpqg dglapllqhn rfaaaaeaap eqqtleqhty
     2641 hhkycpkqgg stynrdqrry acssdqtrpg vptrlaipnf ntqpqqpaln agglgscppf
     2701 lngfpggatg amlggaaqlf snrvaaeaph qeatpwsphk lmgsapgdsl hhhaghhphh
     2761 pqqqpphhah vataklpwqs wthhqt
//
LOCUS       XP_077506484            2775 aa            linear   INV 25-NOV-2025
DEFINITION  multiple ankyrin repeats single KH domain isoform X6 [Amblyomma
            americanum].
ACCESSION   XP_077506484
VERSION     XP_077506484.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650358.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2775
                     /product="multiple ankyrin repeats single KH domain
                     isoform X6"
                     /calculated_mol_wt=287107
     Region          201..466
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          201..227
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          229..260
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          265..293
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(268..269,272..274,276..277,281,284,293,295,297,
                     301..302,305..307,309..310,314,317,326,328,330,334..335,
                     338..340,342..343,347,350)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          295..326
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          328..356
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          362..392
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          376..680
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(397,401..402,405..407,409..410,414,417,426,428,430,
                     434..435,438..440,442..443,447,450,459,461,463,467..468,
                     471..473,475..476,480,483)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          397..426
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          428..459
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          461..491
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          494..526
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          528..556
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(531..532,535..537,539..540,544,547,556,558,560,
                     564..565,568..570,572..573,577,580,589,591,593,597..598,
                     601..603,605..606,610,613)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          558..589
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          591..620
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          625..655
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1150..1175
                     /region_name="ANK"
                     /note="ankyrin repeats; smart00248"
                     /db_xref="CDD:197603"
     Region          1152..1181
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1165..1452
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          1183..1215
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1219,1223..1224,1227..1229,1231..1232,1236,1239,
                     1248,1250,1252,1256..1257,1260..1262,1264..1265,1269,1272,
                     1281,1285,1287,1291..1292,1295..1297,1299..1300,1304,1307)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1219..1248
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1250..1281
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1285..1315
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1319..1349
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1352..1385
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1387..1418
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1420..1451
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1934..2003
                     /region_name="KH-I_MASK"
                     /note="type I K homology (KH) RNA-binding domain found in
                     Mask family proteins; cd22404"
                     /db_xref="CDD:411832"
     Site            order(1943,1945..1947,1949..1953,1956..1957,1960..1961,
                     1966..1969)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411832"
     CDS             1..2775
                     /gene="mask"
                     /coded_by="XM_077650358.1:143..8470"
                     /db_xref="GeneID:144115847"
ORIGIN      
        1 mqnvvhgnac davreepqev skgratgtsd nfetgqrqsl nslhsnfmad ltesdddeas
       61 eaesfildpe eleeervldt skfllssseg gggespelqv gtvdpdtqar lealleatgl
      121 sklslegkqf adpevlrrlt tsvscaldea aaaltrmrae hgggvphqqq qqvqqqpqqq
      181 qqqqqqqsst gvvaasssrt laeacsegdv ravrqlldeg rnvnevteeg esllslacas
      241 gycelaqlll amranvedrg lkdmtplmea ataghvdivr lliehgadvn aqtaqghtpl
      301 mfaclgghea aaralveaga nleehnengh tplmeaasag hvstarvlva agasinthsn
      361 efkesaltla cykghlemvr flleagadqe hktdemhtal measmdghve varllldsga
      421 qvnmptdsfe spltlaacgg hvelamllle rganieevnd egytplmeaa regheemval
      481 llsqgadina qteetqetal tlaccggfle vadfllkaga qlelgastpl meaaqeghle
      541 llrylinaga svnaktatgd taltyaceng htdvadlllq anadlehese ggrtplmkac
      601 raghlctvqf llgrgadpnr ptssndhtpl slacagghls vvelllahga dpshrlkdns
      661 tmlieaskgg htavvqllld ypnlgtaaaa aaaaaaaaaa avtstasatt aaapspavel
      721 cavsltagla pqvvpmteeq rvaalaaava aghpylpqva gavtprvsva aasssaaaps
      781 tdaawppsas tpstsgkrka sgdsssgplv scgggtvvar ggadqlakpg qgqpslrpse
      841 rleacissmm rsaelnhpsr eeqimqkqqi leelqrvere lqgskdltpn edgahacawq
      901 lhtafarqqa eekahaqivl saqhhqhlav aastedvvee lnlrnaalss ssvvvppstg
      961 aaaaeitalp psslpsssta tpstllpaaa krhldkvkas sgkqqlppaa gaavtvpttk
     1021 dastsmvcpp ggasllgsfg aappqaeglm vaapartlhe algeivtgpt aagtqcgggs
     1081 gaqvsitaqq aaavcpdhhh hqhhhhhhhh ggsthpaass ltqqpptlaf hpaafpihaq
     1141 vdlnwqtesn hdtaltlaca gghedlvsll lnrgahlehr dkkgftplml aataghagvv
     1201 dillshgadl eaqsertkdt alslacsggr yevveillar gankehrnvs dytplslaas
     1261 ggyvniikll lqhgaeinsr tgsklgispl mlaamnghva avrllldngs dinaqietnk
     1321 ntaltlacfq grqevvallv drkanvehra ktgltplmea asggyvevgr vlldkgadvn
     1381 appvpssrdt altiaadkgh yafvellikr gaaidvknkk gssplwlacn ggyldvvqll
     1441 vaaqadidsm dnrrvsclma afrrghvkaa kwlvkqvaqf psdqemarfm atlgpdsrdl
     1501 lrkchqctev ihaakerqaa eankcassll eeldlersre esrraaaarr rerkrrkkke
     1561 kqeqmrlakl eaeggdkaas asanngkdsd kdsksegdkq ptssqseddd dddeeeeeed
     1621 trtmaasags srnsriaeld erlpnpggag gnhmatittg ssdrsggqpn apgrpkanga
     1681 sssstpskvr lkeddlppla shesssspss sppkapssap aesknsrkkk qqaaacstae
     1741 rtadpasprl nhtvspspma sskggnsgsk qgkaaqanqa vlqqptstvt rspltvtqss
     1801 tvhrqaavva krggevmvng ladgedfstv vkgtkhrgqg qahhvatdts sktstlgela
     1861 ldvmdscggp rspgngagrp spgvasspkk sssavlstsc stsmttcsaa asaatgtsks
     1921 hhrgeegwke vvrrskkvtv pstaisrvig rcgcninair easgahieve khkgqgerti
     1981 lirgsaeatk qaqlliqglv qepdrdlsdi maqhgvvaps sakapassld hrtttissss
     2041 tttnhtgsss vlvsgssstn klsrqgssak vvgvkaaapa pslppfgqtr paprpgipth
     2101 sggssgnsts vtsssappfa sgsagwgnla ssslastkpa ataatpvtsa savvttapav
     2161 stnpaalngt tttsasytqa vispaksssr aasadprpss spssgggsps ptlgntgsma
     2221 vssaatttsn ttmapsvsts tsstntapai sipqtfspfn nvfsketqps vwgqreaskp
     2281 nfasvaasgv cgvpsssggs vgsvsassep lvdaakapgy rgnlhvsppn fgpigsgprs
     2341 apctppiggg appsppsqpq paaqpqaqef yenasaptta vpqpvqatpq psssrlvfgp
     2401 phyaatapel apppqqqqqq qppqqqqtiq stlnpnapdf ssrsaapvml tapfpqvgts
     2461 hhhqhhhpaa tpshhgmrvp fapqeymhtv qssshmshhh phhhqgaaga lghpgvfplq
     2521 mmshlqqqys glvapvgqmp kgadraplgv rltdegspsa vspqgglspg gglddrklpr
     2581 pigteraqkk gahtsypgiw sysnsaagvg ssgggssltp dlsamqawvq yqhatgpqgd
     2641 glapllqhnr faaaaeaape qqtleqhtyh hqpqqpalna gglgscppfl ngfpggatga
     2701 mlggaaqlfs nrvaaeaphq eatpwsphkl mgsapgdslh hhaghhphhp qqqpphhahv
     2761 ataklpwqsw thhqt
//
LOCUS       XP_077506485            2774 aa            linear   INV 25-NOV-2025
DEFINITION  multiple ankyrin repeats single KH domain isoform X7 [Amblyomma
            americanum].
ACCESSION   XP_077506485
VERSION     XP_077506485.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650359.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2774
                     /product="multiple ankyrin repeats single KH domain
                     isoform X7"
                     /calculated_mol_wt=287036
     Region          201..466
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          201..227
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          229..260
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          265..293
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(268..269,272..274,276..277,281,284,293,295,297,
                     301..302,305..307,309..310,314,317,326,328,330,334..335,
                     338..340,342..343,347,350)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          295..326
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          328..356
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          362..392
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          376..680
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(397,401..402,405..407,409..410,414,417,426,428,430,
                     434..435,438..440,442..443,447,450,459,461,463,467..468,
                     471..473,475..476,480,483)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          397..426
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          428..459
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          461..491
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          494..526
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          528..556
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(531..532,535..537,539..540,544,547,556,558,560,
                     564..565,568..570,572..573,577,580,589,591,593,597..598,
                     601..603,605..606,610,613)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          558..589
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          591..620
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          625..655
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1149..1174
                     /region_name="ANK"
                     /note="ankyrin repeats; smart00248"
                     /db_xref="CDD:197603"
     Region          1151..1180
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1164..1451
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          1182..1214
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1218,1222..1223,1226..1228,1230..1231,1235,1238,
                     1247,1249,1251,1255..1256,1259..1261,1263..1264,1268,1271,
                     1280,1284,1286,1290..1291,1294..1296,1298..1299,1303,1306)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1218..1247
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1249..1280
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1284..1314
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1318..1348
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1351..1384
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1386..1417
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1419..1450
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1933..2002
                     /region_name="KH-I_MASK"
                     /note="type I K homology (KH) RNA-binding domain found in
                     Mask family proteins; cd22404"
                     /db_xref="CDD:411832"
     Site            order(1942,1944..1946,1948..1952,1955..1956,1959..1960,
                     1965..1968)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411832"
     CDS             1..2774
                     /gene="mask"
                     /coded_by="XM_077650359.1:143..8467"
                     /db_xref="GeneID:144115847"
ORIGIN      
        1 mqnvvhgnac davreepqev skgratgtsd nfetgqrqsl nslhsnfmad ltesdddeas
       61 eaesfildpe eleeervldt skfllssseg gggespelqv gtvdpdtqar lealleatgl
      121 sklslegkqf adpevlrrlt tsvscaldea aaaltrmrae hgggvphqqq qqvqqqpqqq
      181 qqqqqqqsst gvvaasssrt laeacsegdv ravrqlldeg rnvnevteeg esllslacas
      241 gycelaqlll amranvedrg lkdmtplmea ataghvdivr lliehgadvn aqtaqghtpl
      301 mfaclgghea aaralveaga nleehnengh tplmeaasag hvstarvlva agasinthsn
      361 efkesaltla cykghlemvr flleagadqe hktdemhtal measmdghve varllldsga
      421 qvnmptdsfe spltlaacgg hvelamllle rganieevnd egytplmeaa regheemval
      481 llsqgadina qteetqetal tlaccggfle vadfllkaga qlelgastpl meaaqeghle
      541 llrylinaga svnaktatgd taltyaceng htdvadlllq anadlehese ggrtplmkac
      601 raghlctvqf llgrgadpnr ptssndhtpl slacagghls vvelllahga dpshrlkdns
      661 tmlieaskgg htavvqllld ypnlgtaaaa aaaaaaaaaa avtstasatt aaapspavel
      721 cavsltagla pqvvpmteeq rvaalaaava aghpylpqva gvtprvsvaa asssaaapst
      781 daawppsast pstsgkrkas gdsssgplvs cgggtvvarg gadqlakpgq gqpslrpser
      841 leacissmmr saelnhpsre eqimqkqqil eelqrverel qgskdltpne dgahacawql
      901 htafarqqae ekahaqivls aqhhqhlava astedvveel nlrnaalsss svvvppstga
      961 aaaeitalpp sslpssstat pstllpaaak rhldkvkass gkqqlppaag aavtvpttkd
     1021 astsmvcppg gasllgsfga appqaeglmv aapartlhea lgeivtgpta agtqcgggsg
     1081 aqvsitaqqa aavcpdhhhh qhhhhhhhhg gsthpaassl tqqpptlafh paafpihaqv
     1141 dlnwqtesnh dtaltlacag ghedlvslll nrgahlehrd kkgftplmla ataghagvvd
     1201 illshgadle aqsertkdta lslacsggry evveillarg ankehrnvsd ytplslaasg
     1261 gyvniiklll qhgaeinsrt gsklgisplm laamnghvaa vrllldngsd inaqietnkn
     1321 taltlacfqg rqevvallvd rkanvehrak tgltplmeaa sggyvevgrv lldkgadvna
     1381 ppvpssrdta ltiaadkghy afvellikrg aaidvknkkg ssplwlacng gyldvvqllv
     1441 aaqadidsmd nrrvsclmaa frrghvkaak wlvkqvaqfp sdqemarfma tlgpdsrdll
     1501 rkchqctevi haakerqaae ankcasslle eldlersree srraaaarrr erkrrkkkek
     1561 qeqmrlakle aeggdkaasa sanngkdsdk dsksegdkqp tssqsedddd ddeeeeeedt
     1621 rtmaasagss rnsriaelde rlpnpggagg nhmatittgs sdrsggqpna pgrpkangas
     1681 ssstpskvrl keddlpplas hesssspsss ppkapssapa esknsrkkkq qaaacstaer
     1741 tadpasprln htvspspmas skggnsgskq gkaaqanqav lqqptstvtr spltvtqsst
     1801 vhrqaavvak rggevmvngl adgedfstvv kgtkhrgqgq ahhvatdtss ktstlgelal
     1861 dvmdscggpr spgngagrps pgvasspkks ssavlstscs tsmttcsaaa saatgtsksh
     1921 hrgeegwkev vrrskkvtvp staisrvigr cgcninaire asgahievek hkgqgertil
     1981 irgsaeatkq aqlliqglvq epdrdlsdim aqhgvvapss akapassldh rtttisssst
     2041 ttnhtgsssv lvsgssstnk lsrqgssakv vgvkaaapap slppfgqtrp aprpgipths
     2101 ggssgnstsv tsssappfas gsagwgnlas sslastkpaa taatpvtsas avvttapavs
     2161 tnpaalngtt ttsasytqav ispaksssra asadprpsss pssgggspsp tlgntgsmav
     2221 ssaatttsnt tmapsvstst sstntapais ipqtfspfnn vfsketqpsv wgqreaskpn
     2281 fasvaasgvc gvpsssggsv gsvsassepl vdaakapgyr gnlhvsppnf gpigsgprsa
     2341 pctppiggga ppsppsqpqp aaqpqaqefy enasapttav pqpvqatpqp sssrlvfgpp
     2401 hyaatapela pppqqqqqqq ppqqqqtiqs tlnpnapdfs srsaapvmlt apfpqvgtsh
     2461 hhqhhhpaat pshhgmrvpf apqeymhtvq ssshmshhhp hhhqgaagal ghpgvfplqm
     2521 mshlqqqysg lvapvgqmpk gadraplgvr ltdegspsav spqgglspgg glddrklprp
     2581 igteraqkkg ahtsypgiws ysnsaagvgs sgggssltpd lsamqawvqy qhatgpqgdg
     2641 lapllqhnrf aaaaeaapeq qtleqhtyhh qpqqpalnag glgscppfln gfpggatgam
     2701 lggaaqlfsn rvaaeaphqe atpwsphklm gsapgdslhh haghhphhpq qqpphhahva
     2761 taklpwqswt hhqt
//
LOCUS       XP_077506486            2773 aa            linear   INV 25-NOV-2025
DEFINITION  multiple ankyrin repeats single KH domain isoform X8 [Amblyomma
            americanum].
ACCESSION   XP_077506486
VERSION     XP_077506486.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650360.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2773
                     /product="multiple ankyrin repeats single KH domain
                     isoform X8"
                     /calculated_mol_wt=287276
     Region          201..466
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          201..227
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          229..260
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          265..293
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(268..269,272..274,276..277,281,284,293,295,297,
                     301..302,305..307,309..310,314,317,326,328,330,334..335,
                     338..340,342..343,347,350)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          295..326
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          328..356
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          362..392
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          376..680
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(397,401..402,405..407,409..410,414,417,426,428,430,
                     434..435,438..440,442..443,447,450,459,461,463,467..468,
                     471..473,475..476,480,483)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          397..426
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          428..459
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          461..491
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          494..526
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          528..556
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(531..532,535..537,539..540,544,547,556,558,560,
                     564..565,568..570,572..573,577,580,589,591,593,597..598,
                     601..603,605..606,610,613)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          558..589
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          591..620
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          625..655
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1108..1133
                     /region_name="ANK"
                     /note="ankyrin repeats; smart00248"
                     /db_xref="CDD:197603"
     Region          1110..1139
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1123..1410
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          1141..1173
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1177,1181..1182,1185..1187,1189..1190,1194,1197,
                     1206,1208,1210,1214..1215,1218..1220,1222..1223,1227,1230,
                     1239,1243,1245,1249..1250,1253..1255,1257..1258,1262,1265)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1177..1206
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1208..1239
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1243..1273
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1277..1307
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1310..1343
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1345..1376
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1378..1409
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1892..1961
                     /region_name="KH-I_MASK"
                     /note="type I K homology (KH) RNA-binding domain found in
                     Mask family proteins; cd22404"
                     /db_xref="CDD:411832"
     Site            order(1901,1903..1905,1907..1911,1914..1915,1918..1919,
                     1924..1927)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411832"
     CDS             1..2773
                     /gene="mask"
                     /coded_by="XM_077650360.1:143..8464"
                     /db_xref="GeneID:144115847"
ORIGIN      
        1 mqnvvhgnac davreepqev skgratgtsd nfetgqrqsl nslhsnfmad ltesdddeas
       61 eaesfildpe eleeervldt skfllssseg gggespelqv gtvdpdtqar lealleatgl
      121 sklslegkqf adpevlrrlt tsvscaldea aaaltrmrae hgggvphqqq qqvqqqpqqq
      181 qqqqqqqsst gvvaasssrt laeacsegdv ravrqlldeg rnvnevteeg esllslacas
      241 gycelaqlll amranvedrg lkdmtplmea ataghvdivr lliehgadvn aqtaqghtpl
      301 mfaclgghea aaralveaga nleehnengh tplmeaasag hvstarvlva agasinthsn
      361 efkesaltla cykghlemvr flleagadqe hktdemhtal measmdghve varllldsga
      421 qvnmptdsfe spltlaacgg hvelamllle rganieevnd egytplmeaa regheemval
      481 llsqgadina qteetqetal tlaccggfle vadfllkaga qlelgastpl meaaqeghle
      541 llrylinaga svnaktatgd taltyaceng htdvadlllq anadlehese ggrtplmkac
      601 raghlctvqf llgrgadpnr ptssndhtpl slacagghls vvelllahga dpshrlkdns
      661 tmlieaskgg htavvqllld ypnlgtaaaa aaaaaaaaaa avtstasatt aaapspavev
      721 vpmteeqrva alaaavaagh pylpqvagav tprvsvaaas ssaaapstda awppsastps
      781 tsgkrkasgd sssgplvscg ggtvvargga dqlakpgqgq pslrpserle acissmmrsa
      841 elnhpsreeq imqkqqilee lqrverelqe kahaqivlsa qhhqhlavaa stedvveeln
      901 lrnaalssss vvvppstgaa aaeitalpps slpssstatp stllpaaakr hldkvkassg
      961 kqqlppaaga avtvpttkda stsmvcppgg asllgsfgaa ppqaeglmva apartlheal
     1021 geivtgptaa gtqcgggsga qvsitaqqaa avcpdhhhhq hhhhhhhhgg sthpaasslt
     1081 qqpptlafhp aafpihaqvd lnwqtesnhd taltlacagg hedlvsllln rgahlehrdk
     1141 kgftplmlaa taghagvvdi llshgadlea qsertkdtal slacsggrye vveillarga
     1201 nkehrnvsdy tplslaasgg yvniiklllq hgaeinsrtg sklgisplml aamnghvaav
     1261 rllldngsdi naqietnknt altlacfqgr qevvallvdr kanvehrakt gltplmeaas
     1321 ggyvevgrvl ldkgadvnap pvpssrdtal tiaadkghya fvellikrga aidvknkkgs
     1381 splwlacngg yldvvqllva aqadidsmdn rrvsclmaaf rrghvkaakw lvkqvaqfps
     1441 dqemarfmat lgpdsrdllr kchqctevih aakerqaaea nkcassllee ldlersrees
     1501 rraaaarrre rkrrkkkekq eqmrlaklea eggdkaasas anngkdsdkd sksegdkqpt
     1561 ssqseddddd deeeeeedtr tmaasagssr nsriaelder lpnpggaggn hmatittgss
     1621 drsggqpnap grpkangass sstpskvrlk eddlpplash esssspsssp pkapssapae
     1681 sknsrkkkqq aaacstaert adpasprlnh tvspspmass kggnsgskqg kaaqanqavl
     1741 qqptstvtrs pltvtqsstv hrqaavvakr ggevmvngla dgedfstvvk gtkhrgqgqa
     1801 hhvatdtssk tstlgelald vmdscggprs pgngagrpsp gvasspkkss savlstscst
     1861 smttcsaaas aatgtskshh rgeegwkevv rrskkvtvps taisrvigrc gcninairea
     1921 sgahievekh kgqgertili rgsaeatkqa qlliqglvqe pdrdlsdima qhgvvapssa
     1981 kapassldhr tttisssstt tnhtgsssvl vsgssstnkl srqgssakvv gvkaaapaps
     2041 lppfgqtrpa prpgipthsg gssgnstsvt sssappfasg sagwgnlass slastkpaat
     2101 aatpvtsasa vvttapavst npaalngttt tsasytqavi spaksssraa sadprpsssp
     2161 ssgggspspt lgntgsmavs saatttsntt mapsvststs stntapaisi pqtfspfnnv
     2221 fsketqpsvw gqreaskpnf asvaasgvcg vpsssggsvg svsasseplv daakapgyrg
     2281 nlhvsppnfg pigsgprsap ctppigggap psppsqpqpa aqpqaqefye nasapttavp
     2341 qpvqatpqps ssrlvfgpph yaatapelap ppqqqqqqqp pqqqqtiqst lnpnapdfss
     2401 rsaapvmlta pfpqvgtshh hqhhhpaatp shhgmrvpfa pqeymhtvqs sshmshhhph
     2461 hhqgaagalg hpgvfplqmm shlqqqysgl vapvgqmpkg adraplgvrl tdegspsavs
     2521 pqgglspggg lddrklprpi gteraqkkga htsypgiwsy snsaagvgss gggssltpdl
     2581 samqawvqyq hatgpqgdgl apllqhnrfa aaaeaapeqq tleqhtyhhk ycpkqggsty
     2641 nrdqrryacs sdqtrpgvpt rlaipnfntq pqqpalnagg lgscppflng fpggatgaml
     2701 ggaaqlfsnr vaaeaphqea tpwsphklmg sapgdslhhh aghhphhpqq qpphhahvat
     2761 aklpwqswth hqt
//
LOCUS       XP_077506487            2773 aa            linear   INV 25-NOV-2025
DEFINITION  multiple ankyrin repeats single KH domain isoform X9 [Amblyomma
            americanum].
ACCESSION   XP_077506487
VERSION     XP_077506487.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650361.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2773
                     /product="multiple ankyrin repeats single KH domain
                     isoform X9"
                     /calculated_mol_wt=287009
     Region          201..466
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          201..227
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          229..260
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          265..293
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(268..269,272..274,276..277,281,284,293,295,297,
                     301..302,305..307,309..310,314,317,326,328,330,334..335,
                     338..340,342..343,347,350)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          295..326
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          328..356
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          362..392
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          376..680
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(397,401..402,405..407,409..410,414,417,426,428,430,
                     434..435,438..440,442..443,447,450,459,461,463,467..468,
                     471..473,475..476,480,483)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          397..426
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          428..459
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          461..491
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          494..526
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          528..556
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(531..532,535..537,539..540,544,547,556,558,560,
                     564..565,568..570,572..573,577,580,589,591,593,597..598,
                     601..603,605..606,610,613)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          558..589
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          591..620
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          625..655
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1106..1131
                     /region_name="ANK"
                     /note="ankyrin repeats; smart00248"
                     /db_xref="CDD:197603"
     Region          1108..1137
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1121..1408
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          1139..1171
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1175,1179..1180,1183..1185,1187..1188,1192,1195,
                     1204,1206,1208,1212..1213,1216..1218,1220..1221,1225,1228,
                     1237,1241,1243,1247..1248,1251..1253,1255..1256,1260,1263)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1175..1204
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1206..1237
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1241..1271
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1275..1305
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1308..1341
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1343..1374
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1376..1407
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1890..1959
                     /region_name="KH-I_MASK"
                     /note="type I K homology (KH) RNA-binding domain found in
                     Mask family proteins; cd22404"
                     /db_xref="CDD:411832"
     Site            order(1899,1901..1903,1905..1909,1912..1913,1916..1917,
                     1922..1925)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411832"
     CDS             1..2773
                     /gene="mask"
                     /coded_by="XM_077650361.1:143..8464"
                     /db_xref="GeneID:144115847"
ORIGIN      
        1 mqnvvhgnac davreepqev skgratgtsd nfetgqrqsl nslhsnfmad ltesdddeas
       61 eaesfildpe eleeervldt skfllssseg gggespelqv gtvdpdtqar lealleatgl
      121 sklslegkqf adpevlrrlt tsvscaldea aaaltrmrae hgggvphqqq qqvqqqpqqq
      181 qqqqqqqsst gvvaasssrt laeacsegdv ravrqlldeg rnvnevteeg esllslacas
      241 gycelaqlll amranvedrg lkdmtplmea ataghvdivr lliehgadvn aqtaqghtpl
      301 mfaclgghea aaralveaga nleehnengh tplmeaasag hvstarvlva agasinthsn
      361 efkesaltla cykghlemvr flleagadqe hktdemhtal measmdghve varllldsga
      421 qvnmptdsfe spltlaacgg hvelamllle rganieevnd egytplmeaa regheemval
      481 llsqgadina qteetqetal tlaccggfle vadfllkaga qlelgastpl meaaqeghle
      541 llrylinaga svnaktatgd taltyaceng htdvadlllq anadlehese ggrtplmkac
      601 raghlctvqf llgrgadpnr ptssndhtpl slacagghls vvelllahga dpshrlkdns
      661 tmlieaskgg htavvqllld ypnlgtaaaa aaaaaaaaaa avtstasatt aaapspavel
      721 cavsltagla pqvvpmteeq rvaalaaava aghpylpqva gavtprvsva aasssaaaps
      781 tdaawppsas tpstsgkrka sgdsssgplv scgggtvvar ggadqlakpg qgqpslrpse
      841 rleacissmm rsaelnhpsr eeqimqkqqi leelqrvere lqhlavaast edvveelnlr
      901 naalssssvv vppstgaaaa eitalppssl pssstatpst llpaaakrhl dkvkassgkq
      961 qlppaagaav tvpttkdast smvcppggas llgsfgaapp qaeglmvaap artlhealge
     1021 ivtgptaagt qcgggsgaqv sitaqqaaav cpdhhhhqhh hhhhhhggst hpaassltqq
     1081 pptlafhpaa fpihaqvdln wqtesnhdta ltlacagghe dlvslllnrg ahlehrdkkg
     1141 ftplmlaata ghagvvdill shgadleaqs ertkdtalsl acsggryevv eillargank
     1201 ehrnvsdytp lslaasggyv niiklllqhg aeinsrtgsk lgisplmlaa mnghvaavrl
     1261 lldngsdina qietnkntal tlacfqgrqe vvallvdrka nvehraktgl tplmeaasgg
     1321 yvevgrvlld kgadvnappv pssrdtalti aadkghyafv ellikrgaai dvknkkgssp
     1381 lwlacnggyl dvvqllvaaq adidsmdnrr vsclmaafrr ghvkaakwlv kqvaqfpsdq
     1441 emarfmatlg pdsrdllrkc hqctevihaa kerqaaeank casslleeld lersreesrr
     1501 aaaarrrerk rrkkkekqeq mrlakleaeg gdkaasasan ngkdsdkdsk segdkqptss
     1561 qsedddddde eeeeedtrtm aasagssrns riaelderlp npggaggnhm atittgssdr
     1621 sggqpnapgr pkangassss tpskvrlked dlpplashes ssspsssppk apssapaesk
     1681 nsrkkkqqaa acstaertad pasprlnhtv spspmasskg gnsgskqgka aqanqavlqq
     1741 ptstvtrspl tvtqsstvhr qaavvakrgg evmvngladg edfstvvkgt khrgqgqahh
     1801 vatdtsskts tlgelaldvm dscggprspg ngagrpspgv asspkksssa vlstscstsm
     1861 ttcsaaasaa tgtskshhrg eegwkevvrr skkvtvpsta isrvigrcgc ninaireasg
     1921 ahievekhkg qgertilirg saeatkqaql liqglvqepd rdlsdimaqh gvvapssaka
     1981 passldhrtt tisssstttn htgsssvlvs gssstnklsr qgssakvvgv kaaapapslp
     2041 pfgqtrpapr pgipthsggs sgnstsvtss sappfasgsa gwgnlasssl astkpaataa
     2101 tpvtsasavv ttapavstnp aalngtttts asytqavisp aksssraasa dprpssspss
     2161 gggspsptlg ntgsmavssa atttsnttma psvststsst ntapaisipq tfspfnnvfs
     2221 ketqpsvwgq reaskpnfas vaasgvcgvp sssggsvgsv sasseplvda akapgyrgnl
     2281 hvsppnfgpi gsgprsapct ppigggapps ppsqpqpaaq pqaqefyena sapttavpqp
     2341 vqatpqpsss rlvfgpphya atapelappp qqqqqqqppq qqqtiqstln pnapdfssrs
     2401 aapvmltapf pqvgtshhhq hhhpaatpsh hgmrvpfapq eymhtvqsss hmshhhphhh
     2461 qgaagalghp gvfplqmmsh lqqqysglva pvgqmpkgad raplgvrltd egspsavspq
     2521 gglspgggld drklprpigt eraqkkgaht sypgiwsysn saagvgssgg gssltpdlsa
     2581 mqawvqyqha tgpqgdglap llqhnrfaaa aeaapeqqtl eqhtyhhkyc pkqggstynr
     2641 dqrryacssd qtrpgvptrl aipnfntvsq pqqpalnagg lgscppflng fpggatgaml
     2701 ggaaqlfsnr vaaeaphqea tpwsphklmg sapgdslhhh aghhphhpqq qpphhahvat
     2761 aklpwqswth hqt
//
LOCUS       XP_077506488            2771 aa            linear   INV 25-NOV-2025
DEFINITION  multiple ankyrin repeats single KH domain isoform X10 [Amblyomma
            americanum].
ACCESSION   XP_077506488
VERSION     XP_077506488.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650362.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2771
                     /product="multiple ankyrin repeats single KH domain
                     isoform X10"
                     /calculated_mol_wt=286823
     Region          201..466
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          201..227
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          229..260
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          265..293
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(268..269,272..274,276..277,281,284,293,295,297,
                     301..302,305..307,309..310,314,317,326,328,330,334..335,
                     338..340,342..343,347,350)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          295..326
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          328..356
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          362..392
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          376..680
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(397,401..402,405..407,409..410,414,417,426,428,430,
                     434..435,438..440,442..443,447,450,459,461,463,467..468,
                     471..473,475..476,480,483)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          397..426
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          428..459
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          461..491
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          494..526
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          528..556
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(531..532,535..537,539..540,544,547,556,558,560,
                     564..565,568..570,572..573,577,580,589,591,593,597..598,
                     601..603,605..606,610,613)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          558..589
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          591..620
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          625..655
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1106..1131
                     /region_name="ANK"
                     /note="ankyrin repeats; smart00248"
                     /db_xref="CDD:197603"
     Region          1108..1137
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1121..1408
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          1139..1171
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1175,1179..1180,1183..1185,1187..1188,1192,1195,
                     1204,1206,1208,1212..1213,1216..1218,1220..1221,1225,1228,
                     1237,1241,1243,1247..1248,1251..1253,1255..1256,1260,1263)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1175..1204
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1206..1237
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1241..1271
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1275..1305
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1308..1341
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1343..1374
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1376..1407
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1890..1959
                     /region_name="KH-I_MASK"
                     /note="type I K homology (KH) RNA-binding domain found in
                     Mask family proteins; cd22404"
                     /db_xref="CDD:411832"
     Site            order(1899,1901..1903,1905..1909,1912..1913,1916..1917,
                     1922..1925)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411832"
     CDS             1..2771
                     /gene="mask"
                     /coded_by="XM_077650362.1:143..8458"
                     /db_xref="GeneID:144115847"
ORIGIN      
        1 mqnvvhgnac davreepqev skgratgtsd nfetgqrqsl nslhsnfmad ltesdddeas
       61 eaesfildpe eleeervldt skfllssseg gggespelqv gtvdpdtqar lealleatgl
      121 sklslegkqf adpevlrrlt tsvscaldea aaaltrmrae hgggvphqqq qqvqqqpqqq
      181 qqqqqqqsst gvvaasssrt laeacsegdv ravrqlldeg rnvnevteeg esllslacas
      241 gycelaqlll amranvedrg lkdmtplmea ataghvdivr lliehgadvn aqtaqghtpl
      301 mfaclgghea aaralveaga nleehnengh tplmeaasag hvstarvlva agasinthsn
      361 efkesaltla cykghlemvr flleagadqe hktdemhtal measmdghve varllldsga
      421 qvnmptdsfe spltlaacgg hvelamllle rganieevnd egytplmeaa regheemval
      481 llsqgadina qteetqetal tlaccggfle vadfllkaga qlelgastpl meaaqeghle
      541 llrylinaga svnaktatgd taltyaceng htdvadlllq anadlehese ggrtplmkac
      601 raghlctvqf llgrgadpnr ptssndhtpl slacagghls vvelllahga dpshrlkdns
      661 tmlieaskgg htavvqllld ypnlgtaaaa aaaaaaaaaa avtstasatt aaapspavel
      721 cavsltagla pqvvpmteeq rvaalaaava aghpylpqva gavtprvsva aasssaaaps
      781 tdaawppsas tpstsgkrka sgdsssgplv scgggtvvar ggadqlakpg qgqpslrpse
      841 rleacissmm rsaelnhpsr eeqimqkqqi leelqrvere lqhlavaast edvveelnlr
      901 naalssssvv vppstgaaaa eitalppssl pssstatpst llpaaakrhl dkvkassgkq
      961 qlppaagaav tvpttkdast smvcppggas llgsfgaapp qaeglmvaap artlhealge
     1021 ivtgptaagt qcgggsgaqv sitaqqaaav cpdhhhhqhh hhhhhhggst hpaassltqq
     1081 pptlafhpaa fpihaqvdln wqtesnhdta ltlacagghe dlvslllnrg ahlehrdkkg
     1141 ftplmlaata ghagvvdill shgadleaqs ertkdtalsl acsggryevv eillargank
     1201 ehrnvsdytp lslaasggyv niiklllqhg aeinsrtgsk lgisplmlaa mnghvaavrl
     1261 lldngsdina qietnkntal tlacfqgrqe vvallvdrka nvehraktgl tplmeaasgg
     1321 yvevgrvlld kgadvnappv pssrdtalti aadkghyafv ellikrgaai dvknkkgssp
     1381 lwlacnggyl dvvqllvaaq adidsmdnrr vsclmaafrr ghvkaakwlv kqvaqfpsdq
     1441 emarfmatlg pdsrdllrkc hqctevihaa kerqaaeank casslleeld lersreesrr
     1501 aaaarrrerk rrkkkekqeq mrlakleaeg gdkaasasan ngkdsdkdsk segdkqptss
     1561 qsedddddde eeeeedtrtm aasagssrns riaelderlp npggaggnhm atittgssdr
     1621 sggqpnapgr pkangassss tpskvrlked dlpplashes ssspsssppk apssapaesk
     1681 nsrkkkqqaa acstaertad pasprlnhtv spspmasskg gnsgskqgka aqanqavlqq
     1741 ptstvtrspl tvtqsstvhr qaavvakrgg evmvngladg edfstvvkgt khrgqgqahh
     1801 vatdtsskts tlgelaldvm dscggprspg ngagrpspgv asspkksssa vlstscstsm
     1861 ttcsaaasaa tgtskshhrg eegwkevvrr skkvtvpsta isrvigrcgc ninaireasg
     1921 ahievekhkg qgertilirg saeatkqaql liqglvqepd rdlsdimaqh gvvapssaka
     1981 passldhrtt tisssstttn htgsssvlvs gssstnklsr qgssakvvgv kaaapapslp
     2041 pfgqtrpapr pgipthsggs sgnstsvtss sappfasgsa gwgnlasssl astkpaataa
     2101 tpvtsasavv ttapavstnp aalngtttts asytqavisp aksssraasa dprpssspss
     2161 gggspsptlg ntgsmavssa atttsnttma psvststsst ntapaisipq tfspfnnvfs
     2221 ketqpsvwgq reaskpnfas vaasgvcgvp sssggsvgsv sasseplvda akapgyrgnl
     2281 hvsppnfgpi gsgprsapct ppigggapps ppsqpqpaaq pqaqefyena sapttavpqp
     2341 vqatpqpsss rlvfgpphya atapelappp qqqqqqqppq qqqtiqstln pnapdfssrs
     2401 aapvmltapf pqvgtshhhq hhhpaatpsh hgmrvpfapq eymhtvqsss hmshhhphhh
     2461 qgaagalghp gvfplqmmsh lqqqysglva pvgqmpkgad raplgvrltd egspsavspq
     2521 gglspgggld drklprpigt eraqkkgaht sypgiwsysn saagvgssgg gssltpdlsa
     2581 mqawvqyqha tgpqgdglap llqhnrfaaa aeaapeqqtl eqhtyhhkyc pkqggstynr
     2641 dqrryacssd qtrpgvptrl aipnfntqpq qpalnagglg scppflngfp ggatgamlgg
     2701 aaqlfsnrva aeaphqeatp wsphklmgsa pgdslhhhag hhphhpqqqp phhahvatak
     2761 lpwqswthhq t
//
LOCUS       XP_077506489            2817 aa            linear   INV 25-NOV-2025
DEFINITION  multiple ankyrin repeats single KH domain isoform X11 [Amblyomma
            americanum].
ACCESSION   XP_077506489
VERSION     XP_077506489.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650363.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2817
                     /product="multiple ankyrin repeats single KH domain
                     isoform X11"
                     /calculated_mol_wt=291824
     Region          201..466
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          201..227
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          229..260
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          265..293
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(268..269,272..274,276..277,281,284,293,295,297,
                     301..302,305..307,309..310,314,317,326,328,330,334..335,
                     338..340,342..343,347,350)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          295..326
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          328..356
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          362..392
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          376..680
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(397,401..402,405..407,409..410,414,417,426,428,430,
                     434..435,438..440,442..443,447,450,459,461,463,467..468,
                     471..473,475..476,480,483)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          397..426
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          428..459
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          461..491
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          494..526
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          528..556
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(531..532,535..537,539..540,544,547,556,558,560,
                     564..565,568..570,572..573,577,580,589,591,593,597..598,
                     601..603,605..606,610,613)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          558..589
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          591..620
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          625..655
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1150..1175
                     /region_name="ANK"
                     /note="ankyrin repeats; smart00248"
                     /db_xref="CDD:197603"
     Region          1152..1181
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1165..1452
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          1183..1215
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1219,1223..1224,1227..1229,1231..1232,1236,1239,
                     1248,1250,1252,1256..1257,1260..1262,1264..1265,1269,1272,
                     1281,1285,1287,1291..1292,1295..1297,1299..1300,1304,1307)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1219..1248
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1250..1281
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1285..1315
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1319..1349
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1352..1385
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1387..1418
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1420..1451
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1934..2003
                     /region_name="KH-I_MASK"
                     /note="type I K homology (KH) RNA-binding domain found in
                     Mask family proteins; cd22404"
                     /db_xref="CDD:411832"
     Site            order(1943,1945..1947,1949..1953,1956..1957,1960..1961,
                     1966..1969)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411832"
     CDS             1..2817
                     /gene="mask"
                     /coded_by="XM_077650363.1:160..8613"
                     /db_xref="GeneID:144115847"
ORIGIN      
        1 mqnvvhgnac davreepqev skgratgtsd nfetgqrqsl nslhsnfmad ltesdddeas
       61 eaesfildpe eleeervldt skfllssseg gggespelqv gtvdpdtqar lealleatgl
      121 sklslegkqf adpevlrrlt tsvscaldea aaaltrmrae hgggvphqqq qqvqqqpqqq
      181 qqqqqqqsst gvvaasssrt laeacsegdv ravrqlldeg rnvnevteeg esllslacas
      241 gycelaqlll amranvedrg lkdmtplmea ataghvdivr lliehgadvn aqtaqghtpl
      301 mfaclgghea aaralveaga nleehnengh tplmeaasag hvstarvlva agasinthsn
      361 efkesaltla cykghlemvr flleagadqe hktdemhtal measmdghve varllldsga
      421 qvnmptdsfe spltlaacgg hvelamllle rganieevnd egytplmeaa regheemval
      481 llsqgadina qteetqetal tlaccggfle vadfllkaga qlelgastpl meaaqeghle
      541 llrylinaga svnaktatgd taltyaceng htdvadlllq anadlehese ggrtplmkac
      601 raghlctvqf llgrgadpnr ptssndhtpl slacagghls vvelllahga dpshrlkdns
      661 tmlieaskgg htavvqllld ypnlgtaaaa aaaaaaaaaa avtstasatt aaapspavel
      721 cavsltagla pqvvpmteeq rvaalaaava aghpylpqva gavtprvsva aasssaaaps
      781 tdaawppsas tpstsgkrka sgdsssgplv scgggtvvar ggadqlakpg qgqpslrpse
      841 rleacissmm rsaelnhpsr eeqimqkqqi leelqrvere lqgskdltpn edgahacawq
      901 lhtafarqqa eekahaqivl saqhhqhlav aastedvvee lnlrnaalss ssvvvppstg
      961 aaaaeitalp psslpsssta tpstllpaaa krhldkvkas sgkqqlppaa gaavtvpttk
     1021 dastsmvcpp ggasllgsfg aappqaeglm vaapartlhe algeivtgpt aagtqcgggs
     1081 gaqvsitaqq aaavcpdhhh hqhhhhhhhh ggsthpaass ltqqpptlaf hpaafpihaq
     1141 vdlnwqtesn hdtaltlaca gghedlvsll lnrgahlehr dkkgftplml aataghagvv
     1201 dillshgadl eaqsertkdt alslacsggr yevveillar gankehrnvs dytplslaas
     1261 ggyvniikll lqhgaeinsr tgsklgispl mlaamnghva avrllldngs dinaqietnk
     1321 ntaltlacfq grqevvallv drkanvehra ktgltplmea asggyvevgr vlldkgadvn
     1381 appvpssrdt altiaadkgh yafvellikr gaaidvknkk gssplwlacn ggyldvvqll
     1441 vaaqadidsm dnrrvsclma afrrghvkaa kwlvkqvaqf psdqemarfm atlgpdsrdl
     1501 lrkchqctev ihaakerqaa eankcassll eeldlersre esrraaaarr rerkrrkkke
     1561 kqeqmrlakl eaeggdkaas asanngkdsd kdsksegdkq ptssqseddd dddeeeeeed
     1621 trtmaasags srnsriaeld erlpnpggag gnhmatittg ssdrsggqpn apgrpkanga
     1681 sssstpskvr lkeddlppla shesssspss sppkapssap aesknsrkkk qqaaacstae
     1741 rtadpasprl nhtvspspma sskggnsgsk qgkaaqanqa vlqqptstvt rspltvtqss
     1801 tvhrqaavva krggevmvng ladgedfstv vkgtkhrgqg qahhvatdts sktstlgela
     1861 ldvmdscggp rspgngagrp spgvasspkk sssavlstsc stsmttcsaa asaatgtsks
     1921 hhrgeegwke vvrrskkvtv pstaisrvig rcgcninair easgahieve khkgqgerti
     1981 lirgsaeatk qaqlliqglv qepdrdlsdi maqhgvvaps sakapassld hrtttissss
     2041 tttnhtgsss vlvsgssstn klsrqgssak vvgvkaaapa pslppfgqtr paprpgipth
     2101 sggssgnsts vtsssappfa sgsagwgnla ssslastkpa ataatpvtsa savvttapav
     2161 stnpaalngt tttsasytqa vispaksssr aasadprpss spssgggsps ptlgntgsma
     2221 vssaatttsn ttmapsvsts tsstntapai sipqtfspfn nvfsketqps vwgqreaskp
     2281 nfasvaasgv cgvpsssggs vgsvsassep lvdaakapgy rgnlhvsppn fgpigsgprs
     2341 apctppiggg appsppsqpq paaqpqaqef yenasaptta vpqpvqatpq psssrlvfgp
     2401 phyaatapel apppqqqqqq qppqqqqtiq stlnpnapdf ssrsaapvml tapfpqvgts
     2461 hhhqhhhpaa tpshhgmrvp fapqeymhtv qssshmshhh phhhqgaaga lghpgvfplq
     2521 mmshlqqqys glvapvgqmp kgadraplgv rltdegspsa vspqgglspg gglddrklpr
     2581 pigteraqkk gahtsypgiw sysnsaagvg ssgggssltp dlsamqawvq yqhatgpqgd
     2641 glapllqhnr faaaaeaape qqtleqhtyh hkycpkqggs tynrdqrrya cssdqtrpgv
     2701 ptrlaipnfn tvsqpqqpal nagglgscpp flngfpggat gamlggaaql fsnrvaaeap
     2761 hqeatpwsph klmgsapgds lhhhaghhph hpqqqpphha hvataklpwq swthhqt
//
LOCUS       XP_077506490            2760 aa            linear   INV 25-NOV-2025
DEFINITION  multiple ankyrin repeats single KH domain isoform X12 [Amblyomma
            americanum].
ACCESSION   XP_077506490
VERSION     XP_077506490.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650364.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2760
                     /product="multiple ankyrin repeats single KH domain
                     isoform X12"
                     /calculated_mol_wt=285428
     Region          201..466
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          201..227
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          229..260
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          265..293
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(268..269,272..274,276..277,281,284,293,295,297,
                     301..302,305..307,309..310,314,317,326,328,330,334..335,
                     338..340,342..343,347,350)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          295..326
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          328..356
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          362..392
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          376..680
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(397,401..402,405..407,409..410,414,417,426,428,430,
                     434..435,438..440,442..443,447,450,459,461,463,467..468,
                     471..473,475..476,480,483)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          397..426
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          428..459
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          461..491
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          494..526
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          528..556
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(531..532,535..537,539..540,544,547,556,558,560,
                     564..565,568..570,572..573,577,580,589,591,593,597..598,
                     601..603,605..606,610,613)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          558..589
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          591..620
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          625..655
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1135..1160
                     /region_name="ANK"
                     /note="ankyrin repeats; smart00248"
                     /db_xref="CDD:197603"
     Region          1137..1166
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1150..1437
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          1168..1200
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1204,1208..1209,1212..1214,1216..1217,1221,1224,
                     1233,1235,1237,1241..1242,1245..1247,1249..1250,1254,1257,
                     1266,1270,1272,1276..1277,1280..1282,1284..1285,1289,1292)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1204..1233
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1235..1266
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1270..1300
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1304..1334
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1337..1370
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1372..1403
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1405..1436
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1919..1988
                     /region_name="KH-I_MASK"
                     /note="type I K homology (KH) RNA-binding domain found in
                     Mask family proteins; cd22404"
                     /db_xref="CDD:411832"
     Site            order(1928,1930..1932,1934..1938,1941..1942,1945..1946,
                     1951..1954)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411832"
     CDS             1..2760
                     /gene="mask"
                     /coded_by="XM_077650364.1:143..8425"
                     /db_xref="GeneID:144115847"
ORIGIN      
        1 mqnvvhgnac davreepqev skgratgtsd nfetgqrqsl nslhsnfmad ltesdddeas
       61 eaesfildpe eleeervldt skfllssseg gggespelqv gtvdpdtqar lealleatgl
      121 sklslegkqf adpevlrrlt tsvscaldea aaaltrmrae hgggvphqqq qqvqqqpqqq
      181 qqqqqqqsst gvvaasssrt laeacsegdv ravrqlldeg rnvnevteeg esllslacas
      241 gycelaqlll amranvedrg lkdmtplmea ataghvdivr lliehgadvn aqtaqghtpl
      301 mfaclgghea aaralveaga nleehnengh tplmeaasag hvstarvlva agasinthsn
      361 efkesaltla cykghlemvr flleagadqe hktdemhtal measmdghve varllldsga
      421 qvnmptdsfe spltlaacgg hvelamllle rganieevnd egytplmeaa regheemval
      481 llsqgadina qteetqetal tlaccggfle vadfllkaga qlelgastpl meaaqeghle
      541 llrylinaga svnaktatgd taltyaceng htdvadlllq anadlehese ggrtplmkac
      601 raghlctvqf llgrgadpnr ptssndhtpl slacagghls vvelllahga dpshrlkdns
      661 tmlieaskgg htavvqllld ypnlgtaaaa aaaaaaaaaa avtstasatt aaapspavel
      721 cavsltagla pqvvpmteeq rvaalaaava aghpylpqva gavtprvsva aasssaaaps
      781 tdaawppsas tpstsgkrka sgdsssgplv scgggtvvar ggadqlakpg qgqpslrpse
      841 rleacissmm rsaelnhpsr eeqimqkqqi leelqrvere lqgskdltpn edgahacawq
      901 lhtafarqqa ehlavaaste dvveelnlrn aalssssvvv ppstgaaaae italppsslp
      961 ssstatpstl lpaaakrhld kvkassgkqq lppaagaavt vpttkdasts mvcppggasl
     1021 lgsfgaappq aeglmvaapa rtlhealgei vtgptaagtq cgggsgaqvs itaqqaaavc
     1081 pdhhhhqhhh hhhhhggsth paassltqqp ptlafhpaaf pihaqvdlnw qtesnhdtal
     1141 tlacagghed lvslllnrga hlehrdkkgf tplmlaatag hagvvdills hgadleaqse
     1201 rtkdtalsla csggryevve illarganke hrnvsdytpl slaasggyvn iiklllqhga
     1261 einsrtgskl gisplmlaam nghvaavrll ldngsdinaq ietnkntalt lacfqgrqev
     1321 vallvdrkan vehraktglt plmeaasggy vevgrvlldk gadvnappvp ssrdtaltia
     1381 adkghyafve llikrgaaid vknkkgsspl wlacnggyld vvqllvaaqa didsmdnrrv
     1441 sclmaafrrg hvkaakwlvk qvaqfpsdqe marfmatlgp dsrdllrkch qctevihaak
     1501 erqaaeankc asslleeldl ersreesrra aaarrrerkr rkkkekqeqm rlakleaegg
     1561 dkaasasann gkdsdkdsks egdkqptssq seddddddee eeeedtrtma asagssrnsr
     1621 iaelderlpn pggaggnhma tittgssdrs ggqpnapgrp kangasssst pskvrlkedd
     1681 lpplashess sspsssppka pssapaeskn srkkkqqaaa cstaertadp asprlnhtvs
     1741 pspmasskgg nsgskqgkaa qanqavlqqp tstvtrsplt vtqsstvhrq aavvakrgge
     1801 vmvngladge dfstvvkgtk hrgqgqahhv atdtssktst lgelaldvmd scggprspgn
     1861 gagrpspgva sspkksssav lstscstsmt tcsaaasaat gtskshhrge egwkevvrrs
     1921 kkvtvpstai srvigrcgcn inaireasga hievekhkgq gertilirgs aeatkqaqll
     1981 iqglvqepdr dlsdimaqhg vvapssakap assldhrttt isssstttnh tgsssvlvsg
     2041 ssstnklsrq gssakvvgvk aaapapslpp fgqtrpaprp gipthsggss gnstsvtsss
     2101 appfasgsag wgnlasssla stkpaataat pvtsasavvt tapavstnpa alngttttsa
     2161 sytqavispa ksssraasad prpssspssg ggspsptlgn tgsmavssaa tttsnttmap
     2221 svststsstn tapaisipqt fspfnnvfsk etqpsvwgqr easkpnfasv aasgvcgvps
     2281 ssggsvgsvs asseplvdaa kapgyrgnlh vsppnfgpig sgprsapctp pigggappsp
     2341 psqpqpaaqp qaqefyenas apttavpqpv qatpqpsssr lvfgpphyaa tapelapppq
     2401 qqqqqqppqq qqtiqstlnp napdfssrsa apvmltapfp qvgtshhhqh hhpaatpshh
     2461 gmrvpfapqe ymhtvqsssh mshhhphhhq gaagalghpg vfplqmmshl qqqysglvap
     2521 vgqmpkgadr aplgvrltde gspsavspqg glspgggldd rklprpigte raqkkgahts
     2581 ypgiwsysns aagvgssggg ssltpdlsam qawvqyqhat gpqgdglapl lqhnrfaaaa
     2641 eaapeqqtle qhtyhhqpqq palnagglgs cppflngfpg gatgamlgga aqlfsnrvaa
     2701 eaphqeatpw sphklmgsap gdslhhhagh hphhpqqqpp hhahvatakl pwqswthhqt
//
LOCUS       XP_077506491            2758 aa            linear   INV 25-NOV-2025
DEFINITION  multiple ankyrin repeats single KH domain isoform X13 [Amblyomma
            americanum].
ACCESSION   XP_077506491
VERSION     XP_077506491.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650365.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2758
                     /product="multiple ankyrin repeats single KH domain
                     isoform X13"
                     /calculated_mol_wt=285444
     Region          142..407
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          142..168
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          170..201
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          206..234
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(209..210,213..215,217..218,222,225,234,236,238,
                     242..243,246..248,250..251,255,258,267,269,271,275..276,
                     279..281,283..284,288,291)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          236..267
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          269..297
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          303..333
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          317..621
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(338,342..343,346..348,350..351,355,358,367,369,371,
                     375..376,379..381,383..384,388,391,400,402,404,408..409,
                     412..414,416..417,421,424)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          338..367
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          369..400
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          402..432
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          435..467
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          469..497
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(472..473,476..478,480..481,485,488,497,499,501,
                     505..506,509..511,513..514,518,521,530,532,534,538..539,
                     542..544,546..547,551,554)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          499..530
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          532..561
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          566..596
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1091..1116
                     /region_name="ANK"
                     /note="ankyrin repeats; smart00248"
                     /db_xref="CDD:197603"
     Region          1093..1122
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1106..1393
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          1124..1156
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1160,1164..1165,1168..1170,1172..1173,1177,1180,
                     1189,1191,1193,1197..1198,1201..1203,1205..1206,1210,1213,
                     1222,1226,1228,1232..1233,1236..1238,1240..1241,1245,1248)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1160..1189
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1191..1222
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1226..1256
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1260..1290
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1293..1326
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1328..1359
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1361..1392
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1875..1944
                     /region_name="KH-I_MASK"
                     /note="type I K homology (KH) RNA-binding domain found in
                     Mask family proteins; cd22404"
                     /db_xref="CDD:411832"
     Site            order(1884,1886..1888,1890..1894,1897..1898,1901..1902,
                     1907..1910)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411832"
     CDS             1..2758
                     /gene="mask"
                     /coded_by="XM_077650365.1:200..8476"
                     /db_xref="GeneID:144115847"
ORIGIN      
        1 mqaesfildp eeleeervld tskfllssse ggggespelq vgtvdpdtqa rlealleatg
       61 lsklslegkq fadpevlrrl ttsvscalde aaaaltrmra ehgggvphqq qqqvqqqpqq
      121 qqqqqqqqss tgvvaasssr tlaeacsegd vravrqllde grnvnevtee gesllslaca
      181 sgycelaqll lamranvedr glkdmtplme aataghvdiv rlliehgadv naqtaqghtp
      241 lmfaclgghe aaaralveag anleehneng htplmeaasa ghvstarvlv aagasinths
      301 nefkesaltl acykghlemv rflleagadq ehktdemhta lmeasmdghv evarllldsg
      361 aqvnmptdsf espltlaacg ghvelamlll erganieevn degytplmea aregheemva
      421 lllsqgadin aqteetqeta ltlaccggfl evadfllkag aqlelgastp lmeaaqeghl
      481 ellrylinag asvnaktatg dtaltyacen ghtdvadlll qanadlehes eggrtplmka
      541 craghlctvq fllgrgadpn rptssndhtp lslacagghl svvelllahg adpshrlkdn
      601 stmlieaskg ghtavvqlll dypnlgtaaa aaaaaaaaaa aavtstasat taaapspave
      661 lcavsltagl apqvvpmtee qrvaalaaav aaghpylpqv agavtprvsv aaasssaaap
      721 stdaawppsa stpstsgkrk asgdsssgpl vscgggtvva rggadqlakp gqgqpslrps
      781 erleacissm mrsaelnhps reeqimqkqq ileelqrver elqgskdltp nedgahacaw
      841 qlhtafarqq aeekahaqiv lsaqhhqhla vaastedvve elnlrnaals sssvvvppst
      901 gaaaaeital ppsslpssst atpstllpaa akrhldkvka ssgkqqlppa agaavtvptt
      961 kdastsmvcp pggasllgsf gaappqaegl mvaapartlh ealgeivtgp taagtqcggg
     1021 sgaqvsitaq qaaavcpdhh hhqhhhhhhh hggsthpaas sltqqpptla fhpaafpiha
     1081 qvdlnwqtes nhdtaltlac agghedlvsl llnrgahleh rdkkgftplm laataghagv
     1141 vdillshgad leaqsertkd talslacsgg ryevveilla rgankehrnv sdytplslaa
     1201 sggyvniikl llqhgaeins rtgsklgisp lmlaamnghv aavrllldng sdinaqietn
     1261 kntaltlacf qgrqevvall vdrkanvehr aktgltplme aasggyvevg rvlldkgadv
     1321 nappvpssrd taltiaadkg hyafvellik rgaaidvknk kgssplwlac nggyldvvql
     1381 lvaaqadids mdnrrvsclm aafrrghvka akwlvkqvaq fpsdqemarf matlgpdsrd
     1441 llrkchqcte vihaakerqa aeankcassl leeldlersr eesrraaaar rrerkrrkkk
     1501 ekqeqmrlak leaeggdkaa sasanngkds dkdsksegdk qptssqsedd ddddeeeeee
     1561 dtrtmaasag ssrnsriael derlpnpgga ggnhmatitt gssdrsggqp napgrpkang
     1621 asssstpskv rlkeddlppl ashessssps ssppkapssa paesknsrkk kqqaaacsta
     1681 ertadpaspr lnhtvspspm asskggnsgs kqgkaaqanq avlqqptstv trspltvtqs
     1741 stvhrqaavv akrggevmvn gladgedfst vvkgtkhrgq gqahhvatdt ssktstlgel
     1801 aldvmdscgg prspgngagr pspgvasspk ksssavlsts cstsmttcsa aasaatgtsk
     1861 shhrgeegwk evvrrskkvt vpstaisrvi grcgcninai reasgahiev ekhkgqgert
     1921 ilirgsaeat kqaqlliqgl vqepdrdlsd imaqhgvvap ssakapassl dhrtttisss
     1981 stttnhtgss svlvsgssst nklsrqgssa kvvgvkaaap apslppfgqt rpaprpgipt
     2041 hsggssgnst svtsssappf asgsagwgnl assslastkp aataatpvts asavvttapa
     2101 vstnpaalng ttttsasytq avispaksss raasadprps sspssgggsp sptlgntgsm
     2161 avssaattts nttmapsvst stsstntapa isipqtfspf nnvfsketqp svwgqreask
     2221 pnfasvaasg vcgvpsssgg svgsvsasse plvdaakapg yrgnlhvspp nfgpigsgpr
     2281 sapctppigg gappsppsqp qpaaqpqaqe fyenasaptt avpqpvqatp qpsssrlvfg
     2341 pphyaatape lapppqqqqq qqppqqqqti qstlnpnapd fssrsaapvm ltapfpqvgt
     2401 shhhqhhhpa atpshhgmrv pfapqeymht vqssshmshh hphhhqgaag alghpgvfpl
     2461 qmmshlqqqy sglvapvgqm pkgadraplg vrltdegsps avspqgglsp ggglddrklp
     2521 rpigteraqk kgahtsypgi wsysnsaagv gssgggsslt pdlsamqawv qyqhatgpqg
     2581 dglapllqhn rfaaaaeaap eqqtleqhty hhkycpkqgg stynrdqrry acssdqtrpg
     2641 vptrlaipnf ntvsqpqqpa lnagglgscp pflngfpgga tgamlggaaq lfsnrvaaea
     2701 phqeatpwsp hklmgsapgd slhhhaghhp hhpqqqpphh ahvataklpw qswthhqt
//
LOCUS       XP_077506492            2757 aa            linear   INV 25-NOV-2025
DEFINITION  multiple ankyrin repeats single KH domain isoform X14 [Amblyomma
            americanum].
ACCESSION   XP_077506492
VERSION     XP_077506492.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650366.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2757
                     /product="multiple ankyrin repeats single KH domain
                     isoform X14"
                     /calculated_mol_wt=285526
     Region          201..466
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          201..227
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          229..260
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          265..293
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(268..269,272..274,276..277,281,284,293,295,297,
                     301..302,305..307,309..310,314,317,326,328,330,334..335,
                     338..340,342..343,347,350)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          295..326
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          328..356
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          362..392
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          376..680
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(397,401..402,405..407,409..410,414,417,426,428,430,
                     434..435,438..440,442..443,447,450,459,461,463,467..468,
                     471..473,475..476,480,483)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          397..426
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          428..459
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          461..491
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          494..526
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          528..556
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(531..532,535..537,539..540,544,547,556,558,560,
                     564..565,568..570,572..573,577,580,589,591,593,597..598,
                     601..603,605..606,610,613)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          558..589
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          591..620
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          625..655
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1092..1117
                     /region_name="ANK"
                     /note="ankyrin repeats; smart00248"
                     /db_xref="CDD:197603"
     Region          1094..1123
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1107..1394
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          1125..1157
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1161,1165..1166,1169..1171,1173..1174,1178,1181,
                     1190,1192,1194,1198..1199,1202..1204,1206..1207,1211,1214,
                     1223,1227,1229,1233..1234,1237..1239,1241..1242,1246,1249)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1161..1190
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1192..1223
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1227..1257
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1261..1291
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1294..1327
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1329..1360
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1362..1393
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1876..1945
                     /region_name="KH-I_MASK"
                     /note="type I K homology (KH) RNA-binding domain found in
                     Mask family proteins; cd22404"
                     /db_xref="CDD:411832"
     Site            order(1885,1887..1889,1891..1895,1898..1899,1902..1903,
                     1908..1911)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411832"
     CDS             1..2757
                     /gene="mask"
                     /coded_by="XM_077650366.1:143..8416"
                     /db_xref="GeneID:144115847"
ORIGIN      
        1 mqnvvhgnac davreepqev skgratgtsd nfetgqrqsl nslhsnfmad ltesdddeas
       61 eaesfildpe eleeervldt skfllssseg gggespelqv gtvdpdtqar lealleatgl
      121 sklslegkqf adpevlrrlt tsvscaldea aaaltrmrae hgggvphqqq qqvqqqpqqq
      181 qqqqqqqsst gvvaasssrt laeacsegdv ravrqlldeg rnvnevteeg esllslacas
      241 gycelaqlll amranvedrg lkdmtplmea ataghvdivr lliehgadvn aqtaqghtpl
      301 mfaclgghea aaralveaga nleehnengh tplmeaasag hvstarvlva agasinthsn
      361 efkesaltla cykghlemvr flleagadqe hktdemhtal measmdghve varllldsga
      421 qvnmptdsfe spltlaacgg hvelamllle rganieevnd egytplmeaa regheemval
      481 llsqgadina qteetqetal tlaccggfle vadfllkaga qlelgastpl meaaqeghle
      541 llrylinaga svnaktatgd taltyaceng htdvadlllq anadlehese ggrtplmkac
      601 raghlctvqf llgrgadpnr ptssndhtpl slacagghls vvelllahga dpshrlkdns
      661 tmlieaskgg htavvqllld ypnlgtaaaa aaaaaaaaaa avtstasatt aaapspavev
      721 vpmteeqrva alaaavaagh pylpqvagvt prvsvaaass saaapstdaa wppsastpst
      781 sgkrkasgds ssgplvscgg gtvvarggad qlakpgqgqp slrpserlea cissmmrsae
      841 lnhpsreeqi mqkqqileel qrverelqhl avaastedvv eelnlrnaal ssssvvvpps
      901 tgaaaaeita lppsslpsss tatpstllpa aakrhldkvk assgkqqlpp aagaavtvpt
      961 tkdastsmvc ppggasllgs fgaappqaeg lmvaapartl healgeivtg ptaagtqcgg
     1021 gsgaqvsita qqaaavcpdh hhhqhhhhhh hhggsthpaa ssltqqpptl afhpaafpih
     1081 aqvdlnwqte snhdtaltla cagghedlvs lllnrgahle hrdkkgftpl mlaataghag
     1141 vvdillshga dleaqsertk dtalslacsg gryevveill argankehrn vsdytplsla
     1201 asggyvniik lllqhgaein srtgsklgis plmlaamngh vaavrllldn gsdinaqiet
     1261 nkntaltlac fqgrqevval lvdrkanveh raktgltplm eaasggyvev grvlldkgad
     1321 vnappvpssr dtaltiaadk ghyafvelli krgaaidvkn kkgssplwla cnggyldvvq
     1381 llvaaqadid smdnrrvscl maafrrghvk aakwlvkqva qfpsdqemar fmatlgpdsr
     1441 dllrkchqct evihaakerq aaeankcass lleeldlers reesrraaaa rrrerkrrkk
     1501 kekqeqmrla kleaeggdka asasanngkd sdkdsksegd kqptssqsed dddddeeeee
     1561 edtrtmaasa gssrnsriae lderlpnpgg aggnhmatit tgssdrsggq pnapgrpkan
     1621 gasssstpsk vrlkeddlpp lashessssp sssppkapss apaesknsrk kkqqaaacst
     1681 aertadpasp rlnhtvspsp masskggnsg skqgkaaqan qavlqqptst vtrspltvtq
     1741 sstvhrqaav vakrggevmv ngladgedfs tvvkgtkhrg qgqahhvatd tssktstlge
     1801 laldvmdscg gprspgngag rpspgvassp kksssavlst scstsmttcs aaasaatgts
     1861 kshhrgeegw kevvrrskkv tvpstaisrv igrcgcnina ireasgahie vekhkgqger
     1921 tilirgsaea tkqaqlliqg lvqepdrdls dimaqhgvva pssakapass ldhrtttiss
     1981 sstttnhtgs ssvlvsgsss tnklsrqgss akvvgvkaaa papslppfgq trpaprpgip
     2041 thsggssgns tsvtsssapp fasgsagwgn lassslastk paataatpvt sasavvttap
     2101 avstnpaaln gttttsasyt qavispakss sraasadprp ssspssgggs psptlgntgs
     2161 mavssaattt snttmapsvs tstsstntap aisipqtfsp fnnvfsketq psvwgqreas
     2221 kpnfasvaas gvcgvpsssg gsvgsvsass eplvdaakap gyrgnlhvsp pnfgpigsgp
     2281 rsapctppig ggappsppsq pqpaaqpqaq efyenasapt tavpqpvqat pqpsssrlvf
     2341 gpphyaatap elapppqqqq qqqppqqqqt iqstlnpnap dfssrsaapv mltapfpqvg
     2401 tshhhqhhhp aatpshhgmr vpfapqeymh tvqssshmsh hhphhhqgaa galghpgvfp
     2461 lqmmshlqqq ysglvapvgq mpkgadrapl gvrltdegsp savspqggls pggglddrkl
     2521 prpigteraq kkgahtsypg iwsysnsaag vgssgggssl tpdlsamqaw vqyqhatgpq
     2581 gdglapllqh nrfaaaaeaa peqqtleqht yhhkycpkqg gstynrdqrr yacssdqtrp
     2641 gvptrlaipn fntqpqqpal nagglgscpp flngfpggat gamlggaaql fsnrvaaeap
     2701 hqeatpwsph klmgsapgds lhhhaghhph hpqqqpphha hvataklpwq swthhqt
//
LOCUS       XP_077506493            2804 aa            linear   INV 25-NOV-2025
DEFINITION  multiple ankyrin repeats single KH domain isoform X15 [Amblyomma
            americanum].
ACCESSION   XP_077506493
VERSION     XP_077506493.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650367.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2804
                     /product="multiple ankyrin repeats single KH domain
                     isoform X15"
                     /calculated_mol_wt=290598
     Region          201..466
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          201..227
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          229..260
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          265..293
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(268..269,272..274,276..277,281,284,293,295,297,
                     301..302,305..307,309..310,314,317,326,328,330,334..335,
                     338..340,342..343,347,350)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          295..326
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          328..356
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          362..392
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          376..680
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(397,401..402,405..407,409..410,414,417,426,428,430,
                     434..435,438..440,442..443,447,450,459,461,463,467..468,
                     471..473,475..476,480,483)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          397..426
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          428..459
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          461..491
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          494..526
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          528..556
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(531..532,535..537,539..540,544,547,556,558,560,
                     564..565,568..570,572..573,577,580,589,591,593,597..598,
                     601..603,605..606,610,613)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          558..589
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          591..620
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          625..655
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1137..1162
                     /region_name="ANK"
                     /note="ankyrin repeats; smart00248"
                     /db_xref="CDD:197603"
     Region          1139..1168
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1152..1439
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          1170..1202
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1206,1210..1211,1214..1216,1218..1219,1223,1226,
                     1235,1237,1239,1243..1244,1247..1249,1251..1252,1256,1259,
                     1268,1272,1274,1278..1279,1282..1284,1286..1287,1291,1294)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1206..1235
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1237..1268
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1272..1302
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1306..1336
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1339..1372
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1374..1405
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1407..1438
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1921..1990
                     /region_name="KH-I_MASK"
                     /note="type I K homology (KH) RNA-binding domain found in
                     Mask family proteins; cd22404"
                     /db_xref="CDD:411832"
     Site            order(1930,1932..1934,1936..1940,1943..1944,1947..1948,
                     1953..1956)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411832"
     CDS             1..2804
                     /gene="mask"
                     /coded_by="XM_077650367.1:160..8574"
                     /db_xref="GeneID:144115847"
ORIGIN      
        1 mqnvvhgnac davreepqev skgratgtsd nfetgqrqsl nslhsnfmad ltesdddeas
       61 eaesfildpe eleeervldt skfllssseg gggespelqv gtvdpdtqar lealleatgl
      121 sklslegkqf adpevlrrlt tsvscaldea aaaltrmrae hgggvphqqq qqvqqqpqqq
      181 qqqqqqqsst gvvaasssrt laeacsegdv ravrqlldeg rnvnevteeg esllslacas
      241 gycelaqlll amranvedrg lkdmtplmea ataghvdivr lliehgadvn aqtaqghtpl
      301 mfaclgghea aaralveaga nleehnengh tplmeaasag hvstarvlva agasinthsn
      361 efkesaltla cykghlemvr flleagadqe hktdemhtal measmdghve varllldsga
      421 qvnmptdsfe spltlaacgg hvelamllle rganieevnd egytplmeaa regheemval
      481 llsqgadina qteetqetal tlaccggfle vadfllkaga qlelgastpl meaaqeghle
      541 llrylinaga svnaktatgd taltyaceng htdvadlllq anadlehese ggrtplmkac
      601 raghlctvqf llgrgadpnr ptssndhtpl slacagghls vvelllahga dpshrlkdns
      661 tmlieaskgg htavvqllld ypnlgtaaaa aaaaaaaaaa avtstasatt aaapspavev
      721 vpmteeqrva alaaavaagh pylpqvagav tprvsvaaas ssaaapstda awppsastps
      781 tsgkrkasgd sssgplvscg ggtvvargga dqlakpgqgq pslrpserle acissmmrsa
      841 elnhpsreeq imqkqqilee lqrverelqg skdltpnedg ahacawqlht afarqqaeek
      901 ahaqivlsaq hhqhlavaas tedvveelnl rnaalssssv vvppstgaaa aeitalppss
      961 lpssstatps tllpaaakrh ldkvkassgk qqlppaagaa vtvpttkdas tsmvcppgga
     1021 sllgsfgaap pqaeglmvaa partlhealg eivtgptaag tqcgggsgaq vsitaqqaaa
     1081 vcpdhhhhqh hhhhhhhggs thpaassltq qpptlafhpa afpihaqvdl nwqtesnhdt
     1141 altlacaggh edlvslllnr gahlehrdkk gftplmlaat aghagvvdil lshgadleaq
     1201 sertkdtals lacsggryev veillargan kehrnvsdyt plslaasggy vniiklllqh
     1261 gaeinsrtgs klgisplmla amnghvaavr llldngsdin aqietnknta ltlacfqgrq
     1321 evvallvdrk anvehraktg ltplmeaasg gyvevgrvll dkgadvnapp vpssrdtalt
     1381 iaadkghyaf vellikrgaa idvknkkgss plwlacnggy ldvvqllvaa qadidsmdnr
     1441 rvsclmaafr rghvkaakwl vkqvaqfpsd qemarfmatl gpdsrdllrk chqcteviha
     1501 akerqaaean kcasslleel dlersreesr raaaarrrer krrkkkekqe qmrlakleae
     1561 ggdkaasasa nngkdsdkds ksegdkqpts sqsedddddd eeeeeedtrt maasagssrn
     1621 sriaelderl pnpggaggnh matittgssd rsggqpnapg rpkangasss stpskvrlke
     1681 ddlpplashe sssspssspp kapssapaes knsrkkkqqa aacstaerta dpasprlnht
     1741 vspspmassk ggnsgskqgk aaqanqavlq qptstvtrsp ltvtqsstvh rqaavvakrg
     1801 gevmvnglad gedfstvvkg tkhrgqgqah hvatdtsskt stlgelaldv mdscggprsp
     1861 gngagrpspg vasspkksss avlstscsts mttcsaaasa atgtskshhr geegwkevvr
     1921 rskkvtvpst aisrvigrcg cninaireas gahievekhk gqgertilir gsaeatkqaq
     1981 lliqglvqep drdlsdimaq hgvvapssak apassldhrt ttissssttt nhtgsssvlv
     2041 sgssstnkls rqgssakvvg vkaaapapsl ppfgqtrpap rpgipthsgg ssgnstsvts
     2101 ssappfasgs agwgnlasss lastkpaata atpvtsasav vttapavstn paalngtttt
     2161 sasytqavis paksssraas adprpsssps sgggspsptl gntgsmavss aatttsnttm
     2221 apsvststss tntapaisip qtfspfnnvf sketqpsvwg qreaskpnfa svaasgvcgv
     2281 psssggsvgs vsasseplvd aakapgyrgn lhvsppnfgp igsgprsapc tppigggapp
     2341 sppsqpqpaa qpqaqefyen asapttavpq pvqatpqpss srlvfgpphy aatapelapp
     2401 pqqqqqqqpp qqqqtiqstl npnapdfssr saapvmltap fpqvgtshhh qhhhpaatps
     2461 hhgmrvpfap qeymhtvqss shmshhhphh hqgaagalgh pgvfplqmms hlqqqysglv
     2521 apvgqmpkga draplgvrlt degspsavsp qgglspgggl ddrklprpig teraqkkgah
     2581 tsypgiwsys nsaagvgssg ggssltpdls amqawvqyqh atgpqgdgla pllqhnrfaa
     2641 aaeaapeqqt leqhtyhhky cpkqggstyn rdqrryacss dqtrpgvptr laipnfntvs
     2701 qpqqpalnag glgscppfln gfpggatgam lggaaqlfsn rvaaeaphqe atpwsphklm
     2761 gsapgdslhh haghhphhpq qqpphhahva taklpwqswt hhqt
//
LOCUS       XP_077506494            2803 aa            linear   INV 25-NOV-2025
DEFINITION  multiple ankyrin repeats single KH domain isoform X16 [Amblyomma
            americanum].
ACCESSION   XP_077506494
VERSION     XP_077506494.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650368.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2803
                     /product="multiple ankyrin repeats single KH domain
                     isoform X16"
                     /calculated_mol_wt=290527
     Region          201..466
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          201..227
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          229..260
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          265..293
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(268..269,272..274,276..277,281,284,293,295,297,
                     301..302,305..307,309..310,314,317,326,328,330,334..335,
                     338..340,342..343,347,350)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          295..326
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          328..356
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          362..392
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          376..680
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(397,401..402,405..407,409..410,414,417,426,428,430,
                     434..435,438..440,442..443,447,450,459,461,463,467..468,
                     471..473,475..476,480,483)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          397..426
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          428..459
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          461..491
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          494..526
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          528..556
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(531..532,535..537,539..540,544,547,556,558,560,
                     564..565,568..570,572..573,577,580,589,591,593,597..598,
                     601..603,605..606,610,613)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          558..589
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          591..620
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          625..655
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1136..1161
                     /region_name="ANK"
                     /note="ankyrin repeats; smart00248"
                     /db_xref="CDD:197603"
     Region          1138..1167
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1151..1438
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          1169..1201
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1205,1209..1210,1213..1215,1217..1218,1222,1225,
                     1234,1236,1238,1242..1243,1246..1248,1250..1251,1255,1258,
                     1267,1271,1273,1277..1278,1281..1283,1285..1286,1290,1293)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1205..1234
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1236..1267
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1271..1301
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1305..1335
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1338..1371
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1373..1404
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1406..1437
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1920..1989
                     /region_name="KH-I_MASK"
                     /note="type I K homology (KH) RNA-binding domain found in
                     Mask family proteins; cd22404"
                     /db_xref="CDD:411832"
     Site            order(1929,1931..1933,1935..1939,1942..1943,1946..1947,
                     1952..1955)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411832"
     CDS             1..2803
                     /gene="mask"
                     /coded_by="XM_077650368.1:160..8571"
                     /db_xref="GeneID:144115847"
ORIGIN      
        1 mqnvvhgnac davreepqev skgratgtsd nfetgqrqsl nslhsnfmad ltesdddeas
       61 eaesfildpe eleeervldt skfllssseg gggespelqv gtvdpdtqar lealleatgl
      121 sklslegkqf adpevlrrlt tsvscaldea aaaltrmrae hgggvphqqq qqvqqqpqqq
      181 qqqqqqqsst gvvaasssrt laeacsegdv ravrqlldeg rnvnevteeg esllslacas
      241 gycelaqlll amranvedrg lkdmtplmea ataghvdivr lliehgadvn aqtaqghtpl
      301 mfaclgghea aaralveaga nleehnengh tplmeaasag hvstarvlva agasinthsn
      361 efkesaltla cykghlemvr flleagadqe hktdemhtal measmdghve varllldsga
      421 qvnmptdsfe spltlaacgg hvelamllle rganieevnd egytplmeaa regheemval
      481 llsqgadina qteetqetal tlaccggfle vadfllkaga qlelgastpl meaaqeghle
      541 llrylinaga svnaktatgd taltyaceng htdvadlllq anadlehese ggrtplmkac
      601 raghlctvqf llgrgadpnr ptssndhtpl slacagghls vvelllahga dpshrlkdns
      661 tmlieaskgg htavvqllld ypnlgtaaaa aaaaaaaaaa avtstasatt aaapspavev
      721 vpmteeqrva alaaavaagh pylpqvagvt prvsvaaass saaapstdaa wppsastpst
      781 sgkrkasgds ssgplvscgg gtvvarggad qlakpgqgqp slrpserlea cissmmrsae
      841 lnhpsreeqi mqkqqileel qrverelqgs kdltpnedga hacawqlhta farqqaeeka
      901 haqivlsaqh hqhlavaast edvveelnlr naalssssvv vppstgaaaa eitalppssl
      961 pssstatpst llpaaakrhl dkvkassgkq qlppaagaav tvpttkdast smvcppggas
     1021 llgsfgaapp qaeglmvaap artlhealge ivtgptaagt qcgggsgaqv sitaqqaaav
     1081 cpdhhhhqhh hhhhhhggst hpaassltqq pptlafhpaa fpihaqvdln wqtesnhdta
     1141 ltlacagghe dlvslllnrg ahlehrdkkg ftplmlaata ghagvvdill shgadleaqs
     1201 ertkdtalsl acsggryevv eillargank ehrnvsdytp lslaasggyv niiklllqhg
     1261 aeinsrtgsk lgisplmlaa mnghvaavrl lldngsdina qietnkntal tlacfqgrqe
     1321 vvallvdrka nvehraktgl tplmeaasgg yvevgrvlld kgadvnappv pssrdtalti
     1381 aadkghyafv ellikrgaai dvknkkgssp lwlacnggyl dvvqllvaaq adidsmdnrr
     1441 vsclmaafrr ghvkaakwlv kqvaqfpsdq emarfmatlg pdsrdllrkc hqctevihaa
     1501 kerqaaeank casslleeld lersreesrr aaaarrrerk rrkkkekqeq mrlakleaeg
     1561 gdkaasasan ngkdsdkdsk segdkqptss qsedddddde eeeeedtrtm aasagssrns
     1621 riaelderlp npggaggnhm atittgssdr sggqpnapgr pkangassss tpskvrlked
     1681 dlpplashes ssspsssppk apssapaesk nsrkkkqqaa acstaertad pasprlnhtv
     1741 spspmasskg gnsgskqgka aqanqavlqq ptstvtrspl tvtqsstvhr qaavvakrgg
     1801 evmvngladg edfstvvkgt khrgqgqahh vatdtsskts tlgelaldvm dscggprspg
     1861 ngagrpspgv asspkksssa vlstscstsm ttcsaaasaa tgtskshhrg eegwkevvrr
     1921 skkvtvpsta isrvigrcgc ninaireasg ahievekhkg qgertilirg saeatkqaql
     1981 liqglvqepd rdlsdimaqh gvvapssaka passldhrtt tisssstttn htgsssvlvs
     2041 gssstnklsr qgssakvvgv kaaapapslp pfgqtrpapr pgipthsggs sgnstsvtss
     2101 sappfasgsa gwgnlasssl astkpaataa tpvtsasavv ttapavstnp aalngtttts
     2161 asytqavisp aksssraasa dprpssspss gggspsptlg ntgsmavssa atttsnttma
     2221 psvststsst ntapaisipq tfspfnnvfs ketqpsvwgq reaskpnfas vaasgvcgvp
     2281 sssggsvgsv sasseplvda akapgyrgnl hvsppnfgpi gsgprsapct ppigggapps
     2341 ppsqpqpaaq pqaqefyena sapttavpqp vqatpqpsss rlvfgpphya atapelappp
     2401 qqqqqqqppq qqqtiqstln pnapdfssrs aapvmltapf pqvgtshhhq hhhpaatpsh
     2461 hgmrvpfapq eymhtvqsss hmshhhphhh qgaagalghp gvfplqmmsh lqqqysglva
     2521 pvgqmpkgad raplgvrltd egspsavspq gglspgggld drklprpigt eraqkkgaht
     2581 sypgiwsysn saagvgssgg gssltpdlsa mqawvqyqha tgpqgdglap llqhnrfaaa
     2641 aeaapeqqtl eqhtyhhkyc pkqggstynr dqrryacssd qtrpgvptrl aipnfntvsq
     2701 pqqpalnagg lgscppflng fpggatgaml ggaaqlfsnr vaaeaphqea tpwsphklmg
     2761 sapgdslhhh aghhphhpqq qpphhahvat aklpwqswth hqt
//
LOCUS       XP_077506495            2746 aa            linear   INV 25-NOV-2025
DEFINITION  multiple ankyrin repeats single KH domain isoform X17 [Amblyomma
            americanum].
ACCESSION   XP_077506495
VERSION     XP_077506495.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650369.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2746
                     /product="multiple ankyrin repeats single KH domain
                     isoform X17"
                     /calculated_mol_wt=283972
     Region          201..466
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          201..227
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          229..260
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          265..293
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(268..269,272..274,276..277,281,284,293,295,297,
                     301..302,305..307,309..310,314,317,326,328,330,334..335,
                     338..340,342..343,347,350)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          295..326
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          328..356
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          362..392
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          376..680
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(397,401..402,405..407,409..410,414,417,426,428,430,
                     434..435,438..440,442..443,447,450,459,461,463,467..468,
                     471..473,475..476,480,483)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          397..426
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          428..459
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          461..491
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          494..526
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          528..556
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(531..532,535..537,539..540,544,547,556,558,560,
                     564..565,568..570,572..573,577,580,589,591,593,597..598,
                     601..603,605..606,610,613)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          558..589
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          591..620
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          625..655
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1121..1146
                     /region_name="ANK"
                     /note="ankyrin repeats; smart00248"
                     /db_xref="CDD:197603"
     Region          1123..1152
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1136..1423
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          1154..1186
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1190,1194..1195,1198..1200,1202..1203,1207,1210,
                     1219,1221,1223,1227..1228,1231..1233,1235..1236,1240,1243,
                     1252,1256,1258,1262..1263,1266..1268,1270..1271,1275,1278)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1190..1219
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1221..1252
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1256..1286
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1290..1320
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1323..1356
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1358..1389
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1391..1422
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1905..1974
                     /region_name="KH-I_MASK"
                     /note="type I K homology (KH) RNA-binding domain found in
                     Mask family proteins; cd22404"
                     /db_xref="CDD:411832"
     Site            order(1914,1916..1918,1920..1924,1927..1928,1931..1932,
                     1937..1940)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411832"
     CDS             1..2746
                     /gene="mask"
                     /coded_by="XM_077650369.1:143..8383"
                     /db_xref="GeneID:144115847"
ORIGIN      
        1 mqnvvhgnac davreepqev skgratgtsd nfetgqrqsl nslhsnfmad ltesdddeas
       61 eaesfildpe eleeervldt skfllssseg gggespelqv gtvdpdtqar lealleatgl
      121 sklslegkqf adpevlrrlt tsvscaldea aaaltrmrae hgggvphqqq qqvqqqpqqq
      181 qqqqqqqsst gvvaasssrt laeacsegdv ravrqlldeg rnvnevteeg esllslacas
      241 gycelaqlll amranvedrg lkdmtplmea ataghvdivr lliehgadvn aqtaqghtpl
      301 mfaclgghea aaralveaga nleehnengh tplmeaasag hvstarvlva agasinthsn
      361 efkesaltla cykghlemvr flleagadqe hktdemhtal measmdghve varllldsga
      421 qvnmptdsfe spltlaacgg hvelamllle rganieevnd egytplmeaa regheemval
      481 llsqgadina qteetqetal tlaccggfle vadfllkaga qlelgastpl meaaqeghle
      541 llrylinaga svnaktatgd taltyaceng htdvadlllq anadlehese ggrtplmkac
      601 raghlctvqf llgrgadpnr ptssndhtpl slacagghls vvelllahga dpshrlkdns
      661 tmlieaskgg htavvqllld ypnlgtaaaa aaaaaaaaaa avtstasatt aaapspavel
      721 cavsltagla pqvvpmteeq rvaalaaava aghpylpqva gavtprvsva aasssaaaps
      781 tdaawppsas tpstsgkrka sgdsssgplv scgggtvvar ggadqlakpg qgqpslrpse
      841 rleacissmm rsaelnhpsr eeqimqkqqi leelqrvere lqekahaqiv lsaqhhqhla
      901 vaastedvve elnlrnaals sssvvvppst gaaaaeital ppsslpssst atpstllpaa
      961 akrhldkvka ssgkqqlppa agaavtvptt kdastsmvcp pggasllgsf gaappqaegl
     1021 mvaapartlh ealgeivtgp taagtqcggg sgaqvsitaq qaaavcpdhh hhqhhhhhhh
     1081 hggsthpaas sltqqpptla fhpaafpiha qvdlnwqtes nhdtaltlac agghedlvsl
     1141 llnrgahleh rdkkgftplm laataghagv vdillshgad leaqsertkd talslacsgg
     1201 ryevveilla rgankehrnv sdytplslaa sggyvniikl llqhgaeins rtgsklgisp
     1261 lmlaamnghv aavrllldng sdinaqietn kntaltlacf qgrqevvall vdrkanvehr
     1321 aktgltplme aasggyvevg rvlldkgadv nappvpssrd taltiaadkg hyafvellik
     1381 rgaaidvknk kgssplwlac nggyldvvql lvaaqadids mdnrrvsclm aafrrghvka
     1441 akwlvkqvaq fpsdqemarf matlgpdsrd llrkchqcte vihaakerqa aeankcassl
     1501 leeldlersr eesrraaaar rrerkrrkkk ekqeqmrlak leaeggdkaa sasanngkds
     1561 dkdsksegdk qptssqsedd ddddeeeeee dtrtmaasag ssrnsriael derlpnpgga
     1621 ggnhmatitt gssdrsggqp napgrpkang asssstpskv rlkeddlppl ashessssps
     1681 ssppkapssa paesknsrkk kqqaaacsta ertadpaspr lnhtvspspm asskggnsgs
     1741 kqgkaaqanq avlqqptstv trspltvtqs stvhrqaavv akrggevmvn gladgedfst
     1801 vvkgtkhrgq gqahhvatdt ssktstlgel aldvmdscgg prspgngagr pspgvasspk
     1861 ksssavlsts cstsmttcsa aasaatgtsk shhrgeegwk evvrrskkvt vpstaisrvi
     1921 grcgcninai reasgahiev ekhkgqgert ilirgsaeat kqaqlliqgl vqepdrdlsd
     1981 imaqhgvvap ssakapassl dhrtttisss stttnhtgss svlvsgssst nklsrqgssa
     2041 kvvgvkaaap apslppfgqt rpaprpgipt hsggssgnst svtsssappf asgsagwgnl
     2101 assslastkp aataatpvts asavvttapa vstnpaalng ttttsasytq avispaksss
     2161 raasadprps sspssgggsp sptlgntgsm avssaattts nttmapsvst stsstntapa
     2221 isipqtfspf nnvfsketqp svwgqreask pnfasvaasg vcgvpsssgg svgsvsasse
     2281 plvdaakapg yrgnlhvspp nfgpigsgpr sapctppigg gappsppsqp qpaaqpqaqe
     2341 fyenasaptt avpqpvqatp qpsssrlvfg pphyaatape lapppqqqqq qqppqqqqti
     2401 qstlnpnapd fssrsaapvm ltapfpqvgt shhhqhhhpa atpshhgmrv pfapqeymht
     2461 vqssshmshh hphhhqgaag alghpgvfpl qmmshlqqqy sglvapvgqm pkgadraplg
     2521 vrltdegsps avspqgglsp ggglddrklp rpigteraqk kgahtsypgi wsysnsaagv
     2581 gssgggsslt pdlsamqawv qyqhatgpqg dglapllqhn rfaaaaeaap eqqtleqhty
     2641 hhqpqqpaln agglgscppf lngfpggatg amlggaaqlf snrvaaeaph qeatpwsphk
     2701 lmgsapgdsl hhhaghhphh pqqqpphhah vataklpwqs wthhqt
//
LOCUS       XP_077506496            2744 aa            linear   INV 25-NOV-2025
DEFINITION  multiple ankyrin repeats single KH domain isoform X18 [Amblyomma
            americanum].
ACCESSION   XP_077506496
VERSION     XP_077506496.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650370.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2744
                     /product="multiple ankyrin repeats single KH domain
                     isoform X18"
                     /calculated_mol_wt=284147
     Region          142..407
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          142..168
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          170..201
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          206..234
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(209..210,213..215,217..218,222,225,234,236,238,
                     242..243,246..248,250..251,255,258,267,269,271,275..276,
                     279..281,283..284,288,291)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          236..267
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          269..297
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          303..333
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          317..621
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(338,342..343,346..348,350..351,355,358,367,369,371,
                     375..376,379..381,383..384,388,391,400,402,404,408..409,
                     412..414,416..417,421,424)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          338..367
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          369..400
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          402..432
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          435..467
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          469..497
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(472..473,476..478,480..481,485,488,497,499,501,
                     505..506,509..511,513..514,518,521,530,532,534,538..539,
                     542..544,546..547,551,554)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          499..530
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          532..561
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          566..596
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1077..1102
                     /region_name="ANK"
                     /note="ankyrin repeats; smart00248"
                     /db_xref="CDD:197603"
     Region          1079..1108
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1092..1379
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          1110..1142
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1146,1150..1151,1154..1156,1158..1159,1163,1166,
                     1175,1177,1179,1183..1184,1187..1189,1191..1192,1196,1199,
                     1208,1212,1214,1218..1219,1222..1224,1226..1227,1231,1234)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1146..1175
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1177..1208
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1212..1242
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1246..1276
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1279..1312
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1314..1345
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1347..1378
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1861..1930
                     /region_name="KH-I_MASK"
                     /note="type I K homology (KH) RNA-binding domain found in
                     Mask family proteins; cd22404"
                     /db_xref="CDD:411832"
     Site            order(1870,1872..1874,1876..1880,1883..1884,1887..1888,
                     1893..1896)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411832"
     CDS             1..2744
                     /gene="mask"
                     /coded_by="XM_077650370.1:277..8511"
                     /db_xref="GeneID:144115847"
ORIGIN      
        1 mqaesfildp eeleeervld tskfllssse ggggespelq vgtvdpdtqa rlealleatg
       61 lsklslegkq fadpevlrrl ttsvscalde aaaaltrmra ehgggvphqq qqqvqqqpqq
      121 qqqqqqqqss tgvvaasssr tlaeacsegd vravrqllde grnvnevtee gesllslaca
      181 sgycelaqll lamranvedr glkdmtplme aataghvdiv rlliehgadv naqtaqghtp
      241 lmfaclgghe aaaralveag anleehneng htplmeaasa ghvstarvlv aagasinths
      301 nefkesaltl acykghlemv rflleagadq ehktdemhta lmeasmdghv evarllldsg
      361 aqvnmptdsf espltlaacg ghvelamlll erganieevn degytplmea aregheemva
      421 lllsqgadin aqteetqeta ltlaccggfl evadfllkag aqlelgastp lmeaaqeghl
      481 ellrylinag asvnaktatg dtaltyacen ghtdvadlll qanadlehes eggrtplmka
      541 craghlctvq fllgrgadpn rptssndhtp lslacagghl svvelllahg adpshrlkdn
      601 stmlieaskg ghtavvqlll dypnlgtaaa aaaaaaaaaa aavtstasat taaapspave
      661 vvpmteeqrv aalaaavaag hpylpqvagv tprvsvaaas ssaaapstda awppsastps
      721 tsgkrkasgd sssgplvscg ggtvvargga dqlakpgqgq pslrpserle acissmmrsa
      781 elnhpsreeq imqkqqilee lqrverelqg skdltpnedg ahacawqlht afarqqaeek
      841 ahaqivlsaq hhqhlavaas tedvveelnl rnaalssssv vvppstgaaa aeitalppss
      901 lpssstatps tllpaaakrh ldkvkassgk qqlppaagaa vtvpttkdas tsmvcppgga
      961 sllgsfgaap pqaeglmvaa partlhealg eivtgptaag tqcgggsgaq vsitaqqaaa
     1021 vcpdhhhhqh hhhhhhhggs thpaassltq qpptlafhpa afpihaqvdl nwqtesnhdt
     1081 altlacaggh edlvslllnr gahlehrdkk gftplmlaat aghagvvdil lshgadleaq
     1141 sertkdtals lacsggryev veillargan kehrnvsdyt plslaasggy vniiklllqh
     1201 gaeinsrtgs klgisplmla amnghvaavr llldngsdin aqietnknta ltlacfqgrq
     1261 evvallvdrk anvehraktg ltplmeaasg gyvevgrvll dkgadvnapp vpssrdtalt
     1321 iaadkghyaf vellikrgaa idvknkkgss plwlacnggy ldvvqllvaa qadidsmdnr
     1381 rvsclmaafr rghvkaakwl vkqvaqfpsd qemarfmatl gpdsrdllrk chqcteviha
     1441 akerqaaean kcasslleel dlersreesr raaaarrrer krrkkkekqe qmrlakleae
     1501 ggdkaasasa nngkdsdkds ksegdkqpts sqsedddddd eeeeeedtrt maasagssrn
     1561 sriaelderl pnpggaggnh matittgssd rsggqpnapg rpkangasss stpskvrlke
     1621 ddlpplashe sssspssspp kapssapaes knsrkkkqqa aacstaerta dpasprlnht
     1681 vspspmassk ggnsgskqgk aaqanqavlq qptstvtrsp ltvtqsstvh rqaavvakrg
     1741 gevmvnglad gedfstvvkg tkhrgqgqah hvatdtsskt stlgelaldv mdscggprsp
     1801 gngagrpspg vasspkksss avlstscsts mttcsaaasa atgtskshhr geegwkevvr
     1861 rskkvtvpst aisrvigrcg cninaireas gahievekhk gqgertilir gsaeatkqaq
     1921 lliqglvqep drdlsdimaq hgvvapssak apassldhrt ttissssttt nhtgsssvlv
     1981 sgssstnkls rqgssakvvg vkaaapapsl ppfgqtrpap rpgipthsgg ssgnstsvts
     2041 ssappfasgs agwgnlasss lastkpaata atpvtsasav vttapavstn paalngtttt
     2101 sasytqavis paksssraas adprpsssps sgggspsptl gntgsmavss aatttsnttm
     2161 apsvststss tntapaisip qtfspfnnvf sketqpsvwg qreaskpnfa svaasgvcgv
     2221 psssggsvgs vsasseplvd aakapgyrgn lhvsppnfgp igsgprsapc tppigggapp
     2281 sppsqpqpaa qpqaqefyen asapttavpq pvqatpqpss srlvfgpphy aatapelapp
     2341 pqqqqqqqpp qqqqtiqstl npnapdfssr saapvmltap fpqvgtshhh qhhhpaatps
     2401 hhgmrvpfap qeymhtvqss shmshhhphh hqgaagalgh pgvfplqmms hlqqqysglv
     2461 apvgqmpkga draplgvrlt degspsavsp qgglspgggl ddrklprpig teraqkkgah
     2521 tsypgiwsys nsaagvgssg ggssltpdls amqawvqyqh atgpqgdgla pllqhnrfaa
     2581 aaeaapeqqt leqhtyhhky cpkqggstyn rdqrryacss dqtrpgvptr laipnfntvs
     2641 qpqqpalnag glgscppfln gfpggatgam lggaaqlfsn rvaaeaphqe atpwsphklm
     2701 gsapgdslhh haghhphhpq qqpphhahva taklpwqswt hhqt
//
LOCUS       XP_077506497            2788 aa            linear   INV 25-NOV-2025
DEFINITION  multiple ankyrin repeats single KH domain isoform X19 [Amblyomma
            americanum].
ACCESSION   XP_077506497
VERSION     XP_077506497.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650371.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2788
                     /product="multiple ankyrin repeats single KH domain
                     isoform X19"
                     /calculated_mol_wt=288688
     Region          201..466
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          201..227
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          229..260
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          265..293
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(268..269,272..274,276..277,281,284,293,295,297,
                     301..302,305..307,309..310,314,317,326,328,330,334..335,
                     338..340,342..343,347,350)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          295..326
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          328..356
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          362..392
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          376..680
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(397,401..402,405..407,409..410,414,417,426,428,430,
                     434..435,438..440,442..443,447,450,459,461,463,467..468,
                     471..473,475..476,480,483)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          397..426
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          428..459
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          461..491
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          494..526
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          528..556
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(531..532,535..537,539..540,544,547,556,558,560,
                     564..565,568..570,572..573,577,580,589,591,593,597..598,
                     601..603,605..606,610,613)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          558..589
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          591..620
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          625..655
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1121..1146
                     /region_name="ANK"
                     /note="ankyrin repeats; smart00248"
                     /db_xref="CDD:197603"
     Region          1123..1152
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1136..1423
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          1154..1186
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1190,1194..1195,1198..1200,1202..1203,1207,1210,
                     1219,1221,1223,1227..1228,1231..1233,1235..1236,1240,1243,
                     1252,1256,1258,1262..1263,1266..1268,1270..1271,1275,1278)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1190..1219
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1221..1252
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1256..1286
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1290..1320
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1323..1356
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1358..1389
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1391..1422
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1905..1974
                     /region_name="KH-I_MASK"
                     /note="type I K homology (KH) RNA-binding domain found in
                     Mask family proteins; cd22404"
                     /db_xref="CDD:411832"
     Site            order(1914,1916..1918,1920..1924,1927..1928,1931..1932,
                     1937..1940)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411832"
     CDS             1..2788
                     /gene="mask"
                     /coded_by="XM_077650371.1:160..8526"
                     /db_xref="GeneID:144115847"
ORIGIN      
        1 mqnvvhgnac davreepqev skgratgtsd nfetgqrqsl nslhsnfmad ltesdddeas
       61 eaesfildpe eleeervldt skfllssseg gggespelqv gtvdpdtqar lealleatgl
      121 sklslegkqf adpevlrrlt tsvscaldea aaaltrmrae hgggvphqqq qqvqqqpqqq
      181 qqqqqqqsst gvvaasssrt laeacsegdv ravrqlldeg rnvnevteeg esllslacas
      241 gycelaqlll amranvedrg lkdmtplmea ataghvdivr lliehgadvn aqtaqghtpl
      301 mfaclgghea aaralveaga nleehnengh tplmeaasag hvstarvlva agasinthsn
      361 efkesaltla cykghlemvr flleagadqe hktdemhtal measmdghve varllldsga
      421 qvnmptdsfe spltlaacgg hvelamllle rganieevnd egytplmeaa regheemval
      481 llsqgadina qteetqetal tlaccggfle vadfllkaga qlelgastpl meaaqeghle
      541 llrylinaga svnaktatgd taltyaceng htdvadlllq anadlehese ggrtplmkac
      601 raghlctvqf llgrgadpnr ptssndhtpl slacagghls vvelllahga dpshrlkdns
      661 tmlieaskgg htavvqllld ypnlgtaaaa aaaaaaaaaa avtstasatt aaapspavel
      721 cavsltagla pqvvpmteeq rvaalaaava aghpylpqva gavtprvsva aasssaaaps
      781 tdaawppsas tpstsgkrka sgdsssgplv scgggtvvar ggadqlakpg qgqpslrpse
      841 rleacissmm rsaelnhpsr eeqimqkqqi leelqrvere lqekahaqiv lsaqhhqhla
      901 vaastedvve elnlrnaals sssvvvppst gaaaaeital ppsslpssst atpstllpaa
      961 akrhldkvka ssgkqqlppa agaavtvptt kdastsmvcp pggasllgsf gaappqaegl
     1021 mvaapartlh ealgeivtgp taagtqcggg sgaqvsitaq qaaavcpdhh hhqhhhhhhh
     1081 hggsthpaas sltqqpptla fhpaafpiha qvdlnwqtes nhdtaltlac agghedlvsl
     1141 llnrgahleh rdkkgftplm laataghagv vdillshgad leaqsertkd talslacsgg
     1201 ryevveilla rgankehrnv sdytplslaa sggyvniikl llqhgaeins rtgsklgisp
     1261 lmlaamnghv aavrllldng sdinaqietn kntaltlacf qgrqevvall vdrkanvehr
     1321 aktgltplme aasggyvevg rvlldkgadv nappvpssrd taltiaadkg hyafvellik
     1381 rgaaidvknk kgssplwlac nggyldvvql lvaaqadids mdnrrvsclm aafrrghvka
     1441 akwlvkqvaq fpsdqemarf matlgpdsrd llrkchqcte vihaakerqa aeankcassl
     1501 leeldlersr eesrraaaar rrerkrrkkk ekqeqmrlak leaeggdkaa sasanngkds
     1561 dkdsksegdk qptssqsedd ddddeeeeee dtrtmaasag ssrnsriael derlpnpgga
     1621 ggnhmatitt gssdrsggqp napgrpkang asssstpskv rlkeddlppl ashessssps
     1681 ssppkapssa paesknsrkk kqqaaacsta ertadpaspr lnhtvspspm asskggnsgs
     1741 kqgkaaqanq avlqqptstv trspltvtqs stvhrqaavv akrggevmvn gladgedfst
     1801 vvkgtkhrgq gqahhvatdt ssktstlgel aldvmdscgg prspgngagr pspgvasspk
     1861 ksssavlsts cstsmttcsa aasaatgtsk shhrgeegwk evvrrskkvt vpstaisrvi
     1921 grcgcninai reasgahiev ekhkgqgert ilirgsaeat kqaqlliqgl vqepdrdlsd
     1981 imaqhgvvap ssakapassl dhrtttisss stttnhtgss svlvsgssst nklsrqgssa
     2041 kvvgvkaaap apslppfgqt rpaprpgipt hsggssgnst svtsssappf asgsagwgnl
     2101 assslastkp aataatpvts asavvttapa vstnpaalng ttttsasytq avispaksss
     2161 raasadprps sspssgggsp sptlgntgsm avssaattts nttmapsvst stsstntapa
     2221 isipqtfspf nnvfsketqp svwgqreask pnfasvaasg vcgvpsssgg svgsvsasse
     2281 plvdaakapg yrgnlhvspp nfgpigsgpr sapctppigg gappsppsqp qpaaqpqaqe
     2341 fyenasaptt avpqpvqatp qpsssrlvfg pphyaatape lapppqqqqq qqppqqqqti
     2401 qstlnpnapd fssrsaapvm ltapfpqvgt shhhqhhhpa atpshhgmrv pfapqeymht
     2461 vqssshmshh hphhhqgaag alghpgvfpl qmmshlqqqy sglvapvgqm pkgadraplg
     2521 vrltdegsps avspqgglsp ggglddrklp rpigteraqk kgahtsypgi wsysnsaagv
     2581 gssgggsslt pdlsamqawv qyqhatgpqg dglapllqhn rfaaaaeaap eqqtleqhty
     2641 hhkycpkqgg stynrdqrry acssdqtrpg vptrlaipnf ntvsqpqqpa lnagglgscp
     2701 pflngfpgga tgamlggaaq lfsnrvaaea phqeatpwsp hklmgsapgd slhhhaghhp
     2761 hhpqqqpphh ahvataklpw qswthhqt
//
LOCUS       XP_077506498            2734 aa            linear   INV 25-NOV-2025
DEFINITION  multiple ankyrin repeats single KH domain isoform X20 [Amblyomma
            americanum].
ACCESSION   XP_077506498
VERSION     XP_077506498.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650372.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2734
                     /product="multiple ankyrin repeats single KH domain
                     isoform X20"
                     /calculated_mol_wt=282952
     Region          201..466
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          201..227
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          229..260
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          265..293
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(268..269,272..274,276..277,281,284,293,295,297,
                     301..302,305..307,309..310,314,317,326,328,330,334..335,
                     338..340,342..343,347,350)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          295..326
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          328..356
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          362..392
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          376..680
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(397,401..402,405..407,409..410,414,417,426,428,430,
                     434..435,438..440,442..443,447,450,459,461,463,467..468,
                     471..473,475..476,480,483)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          397..426
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          428..459
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          461..491
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          494..526
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          528..556
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(531..532,535..537,539..540,544,547,556,558,560,
                     564..565,568..570,572..573,577,580,589,591,593,597..598,
                     601..603,605..606,610,613)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          558..589
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          591..620
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          625..655
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1067..1092
                     /region_name="ANK"
                     /note="ankyrin repeats; smart00248"
                     /db_xref="CDD:197603"
     Region          1069..1098
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1082..1369
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          1100..1132
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1136,1140..1141,1144..1146,1148..1149,1153,1156,
                     1165,1167,1169,1173..1174,1177..1179,1181..1182,1186,1189,
                     1198,1202,1204,1208..1209,1212..1214,1216..1217,1221,1224)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1136..1165
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1167..1198
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1202..1232
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1236..1266
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1269..1302
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1304..1335
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1337..1368
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1851..1920
                     /region_name="KH-I_MASK"
                     /note="type I K homology (KH) RNA-binding domain found in
                     Mask family proteins; cd22404"
                     /db_xref="CDD:411832"
     Site            order(1860,1862..1864,1866..1870,1873..1874,1877..1878,
                     1883..1886)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411832"
     CDS             1..2734
                     /gene="mask"
                     /coded_by="XM_077650372.1:143..8347"
                     /db_xref="GeneID:144115847"
ORIGIN      
        1 mqnvvhgnac davreepqev skgratgtsd nfetgqrqsl nslhsnfmad ltesdddeas
       61 eaesfildpe eleeervldt skfllssseg gggespelqv gtvdpdtqar lealleatgl
      121 sklslegkqf adpevlrrlt tsvscaldea aaaltrmrae hgggvphqqq qqvqqqpqqq
      181 qqqqqqqsst gvvaasssrt laeacsegdv ravrqlldeg rnvnevteeg esllslacas
      241 gycelaqlll amranvedrg lkdmtplmea ataghvdivr lliehgadvn aqtaqghtpl
      301 mfaclgghea aaralveaga nleehnengh tplmeaasag hvstarvlva agasinthsn
      361 efkesaltla cykghlemvr flleagadqe hktdemhtal measmdghve varllldsga
      421 qvnmptdsfe spltlaacgg hvelamllle rganieevnd egytplmeaa regheemval
      481 llsqgadina qteetqetal tlaccggfle vadfllkaga qlelgastpl meaaqeghle
      541 llrylinaga svnaktatgd taltyaceng htdvadlllq anadlehese ggrtplmkac
      601 raghlctvqf llgrgadpnr ptssndhtpl slacagghls vvelllahga dpshrlkdns
      661 tmlieaskgg htavvqllld ypnlgtaaaa aaaaaaaaaa avtstasatt aaapspavel
      721 cavsltagla pqvvpmteeq rvaalaaava aghpylpqva gavtprvsva aasssaaaps
      781 tdaawppsas tpstsgkrkg skdltpnedg ahacawqlht afarqqaeek ahaqivlsaq
      841 hhqhlavaas tedvveelnl rnaalssssv vvppstgaaa aeitalppss lpssstatps
      901 tllpaaakrh ldkvkassgk qqlppaagaa vtvpttkdas tsmvcppgga sllgsfgaap
      961 pqaeglmvaa partlhealg eivtgptaag tqcgggsgaq vsitaqqaaa vcpdhhhhqh
     1021 hhhhhhhggs thpaassltq qpptlafhpa afpihaqvdl nwqtesnhdt altlacaggh
     1081 edlvslllnr gahlehrdkk gftplmlaat aghagvvdil lshgadleaq sertkdtals
     1141 lacsggryev veillargan kehrnvsdyt plslaasggy vniiklllqh gaeinsrtgs
     1201 klgisplmla amnghvaavr llldngsdin aqietnknta ltlacfqgrq evvallvdrk
     1261 anvehraktg ltplmeaasg gyvevgrvll dkgadvnapp vpssrdtalt iaadkghyaf
     1321 vellikrgaa idvknkkgss plwlacnggy ldvvqllvaa qadidsmdnr rvsclmaafr
     1381 rghvkaakwl vkqvaqfpsd qemarfmatl gpdsrdllrk chqcteviha akerqaaean
     1441 kcasslleel dlersreesr raaaarrrer krrkkkekqe qmrlakleae ggdkaasasa
     1501 nngkdsdkds ksegdkqpts sqsedddddd eeeeeedtrt maasagssrn sriaelderl
     1561 pnpggaggnh matittgssd rsggqpnapg rpkangasss stpskvrlke ddlpplashe
     1621 sssspssspp kapssapaes knsrkkkqqa aacstaerta dpasprlnht vspspmassk
     1681 ggnsgskqgk aaqanqavlq qptstvtrsp ltvtqsstvh rqaavvakrg gevmvnglad
     1741 gedfstvvkg tkhrgqgqah hvatdtsskt stlgelaldv mdscggprsp gngagrpspg
     1801 vasspkksss avlstscsts mttcsaaasa atgtskshhr geegwkevvr rskkvtvpst
     1861 aisrvigrcg cninaireas gahievekhk gqgertilir gsaeatkqaq lliqglvqep
     1921 drdlsdimaq hgvvapssak apassldhrt ttissssttt nhtgsssvlv sgssstnkls
     1981 rqgssakvvg vkaaapapsl ppfgqtrpap rpgipthsgg ssgnstsvts ssappfasgs
     2041 agwgnlasss lastkpaata atpvtsasav vttapavstn paalngtttt sasytqavis
     2101 paksssraas adprpsssps sgggspsptl gntgsmavss aatttsnttm apsvststss
     2161 tntapaisip qtfspfnnvf sketqpsvwg qreaskpnfa svaasgvcgv psssggsvgs
     2221 vsasseplvd aakapgyrgn lhvsppnfgp igsgprsapc tppigggapp sppsqpqpaa
     2281 qpqaqefyen asapttavpq pvqatpqpss srlvfgpphy aatapelapp pqqqqqqqpp
     2341 qqqqtiqstl npnapdfssr saapvmltap fpqvgtshhh qhhhpaatps hhgmrvpfap
     2401 qeymhtvqss shmshhhphh hqgaagalgh pgvfplqmms hlqqqysglv apvgqmpkga
     2461 draplgvrlt degspsavsp qgglspgggl ddrklprpig teraqkkgah tsypgiwsys
     2521 nsaagvgssg ggssltpdls amqawvqyqh atgpqgdgla pllqhnrfaa aaeaapeqqt
     2581 leqhtyhhky cpkqggstyn rdqrryacss dqtrpgvptr laipnfntvs qpqqpalnag
     2641 glgscppfln gfpggatgam lggaaqlfsn rvaaeaphqe atpwsphklm gsapgdslhh
     2701 haghhphhpq qqpphhahva taklpwqswt hhqt
//
LOCUS       XP_077506499            2731 aa            linear   INV 25-NOV-2025
DEFINITION  multiple ankyrin repeats single KH domain isoform X21 [Amblyomma
            americanum].
ACCESSION   XP_077506499
VERSION     XP_077506499.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650373.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2731
                     /product="multiple ankyrin repeats single KH domain
                     isoform X21"
                     /calculated_mol_wt=282293
     Region          201..466
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          201..227
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          229..260
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          265..293
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(268..269,272..274,276..277,281,284,293,295,297,
                     301..302,305..307,309..310,314,317,326,328,330,334..335,
                     338..340,342..343,347,350)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          295..326
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          328..356
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          362..392
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          376..680
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(397,401..402,405..407,409..410,414,417,426,428,430,
                     434..435,438..440,442..443,447,450,459,461,463,467..468,
                     471..473,475..476,480,483)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          397..426
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          428..459
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          461..491
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          494..526
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          528..556
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(531..532,535..537,539..540,544,547,556,558,560,
                     564..565,568..570,572..573,577,580,589,591,593,597..598,
                     601..603,605..606,610,613)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          558..589
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          591..620
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          625..655
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1106..1131
                     /region_name="ANK"
                     /note="ankyrin repeats; smart00248"
                     /db_xref="CDD:197603"
     Region          1108..1137
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1121..1408
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          1139..1171
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1175,1179..1180,1183..1185,1187..1188,1192,1195,
                     1204,1206,1208,1212..1213,1216..1218,1220..1221,1225,1228,
                     1237,1241,1243,1247..1248,1251..1253,1255..1256,1260,1263)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1175..1204
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1206..1237
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1241..1271
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1275..1305
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1308..1341
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1343..1374
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1376..1407
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1890..1959
                     /region_name="KH-I_MASK"
                     /note="type I K homology (KH) RNA-binding domain found in
                     Mask family proteins; cd22404"
                     /db_xref="CDD:411832"
     Site            order(1899,1901..1903,1905..1909,1912..1913,1916..1917,
                     1922..1925)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411832"
     CDS             1..2731
                     /gene="mask"
                     /coded_by="XM_077650373.1:143..8338"
                     /db_xref="GeneID:144115847"
ORIGIN      
        1 mqnvvhgnac davreepqev skgratgtsd nfetgqrqsl nslhsnfmad ltesdddeas
       61 eaesfildpe eleeervldt skfllssseg gggespelqv gtvdpdtqar lealleatgl
      121 sklslegkqf adpevlrrlt tsvscaldea aaaltrmrae hgggvphqqq qqvqqqpqqq
      181 qqqqqqqsst gvvaasssrt laeacsegdv ravrqlldeg rnvnevteeg esllslacas
      241 gycelaqlll amranvedrg lkdmtplmea ataghvdivr lliehgadvn aqtaqghtpl
      301 mfaclgghea aaralveaga nleehnengh tplmeaasag hvstarvlva agasinthsn
      361 efkesaltla cykghlemvr flleagadqe hktdemhtal measmdghve varllldsga
      421 qvnmptdsfe spltlaacgg hvelamllle rganieevnd egytplmeaa regheemval
      481 llsqgadina qteetqetal tlaccggfle vadfllkaga qlelgastpl meaaqeghle
      541 llrylinaga svnaktatgd taltyaceng htdvadlllq anadlehese ggrtplmkac
      601 raghlctvqf llgrgadpnr ptssndhtpl slacagghls vvelllahga dpshrlkdns
      661 tmlieaskgg htavvqllld ypnlgtaaaa aaaaaaaaaa avtstasatt aaapspavel
      721 cavsltagla pqvvpmteeq rvaalaaava aghpylpqva gavtprvsva aasssaaaps
      781 tdaawppsas tpstsgkrka sgdsssgplv scgggtvvar ggadqlakpg qgqpslrpse
      841 rleacissmm rsaelnhpsr eeqimqkqqi leelqrvere lqhlavaast edvveelnlr
      901 naalssssvv vppstgaaaa eitalppssl pssstatpst llpaaakrhl dkvkassgkq
      961 qlppaagaav tvpttkdast smvcppggas llgsfgaapp qaeglmvaap artlhealge
     1021 ivtgptaagt qcgggsgaqv sitaqqaaav cpdhhhhqhh hhhhhhggst hpaassltqq
     1081 pptlafhpaa fpihaqvdln wqtesnhdta ltlacagghe dlvslllnrg ahlehrdkkg
     1141 ftplmlaata ghagvvdill shgadleaqs ertkdtalsl acsggryevv eillargank
     1201 ehrnvsdytp lslaasggyv niiklllqhg aeinsrtgsk lgisplmlaa mnghvaavrl
     1261 lldngsdina qietnkntal tlacfqgrqe vvallvdrka nvehraktgl tplmeaasgg
     1321 yvevgrvlld kgadvnappv pssrdtalti aadkghyafv ellikrgaai dvknkkgssp
     1381 lwlacnggyl dvvqllvaaq adidsmdnrr vsclmaafrr ghvkaakwlv kqvaqfpsdq
     1441 emarfmatlg pdsrdllrkc hqctevihaa kerqaaeank casslleeld lersreesrr
     1501 aaaarrrerk rrkkkekqeq mrlakleaeg gdkaasasan ngkdsdkdsk segdkqptss
     1561 qsedddddde eeeeedtrtm aasagssrns riaelderlp npggaggnhm atittgssdr
     1621 sggqpnapgr pkangassss tpskvrlked dlpplashes ssspsssppk apssapaesk
     1681 nsrkkkqqaa acstaertad pasprlnhtv spspmasskg gnsgskqgka aqanqavlqq
     1741 ptstvtrspl tvtqsstvhr qaavvakrgg evmvngladg edfstvvkgt khrgqgqahh
     1801 vatdtsskts tlgelaldvm dscggprspg ngagrpspgv asspkksssa vlstscstsm
     1861 ttcsaaasaa tgtskshhrg eegwkevvrr skkvtvpsta isrvigrcgc ninaireasg
     1921 ahievekhkg qgertilirg saeatkqaql liqglvqepd rdlsdimaqh gvvapssaka
     1981 passldhrtt tisssstttn htgsssvlvs gssstnklsr qgssakvvgv kaaapapslp
     2041 pfgqtrpapr pgipthsggs sgnstsvtss sappfasgsa gwgnlasssl astkpaataa
     2101 tpvtsasavv ttapavstnp aalngtttts asytqavisp aksssraasa dprpssspss
     2161 gggspsptlg ntgsmavssa atttsnttma psvststsst ntapaisipq tfspfnnvfs
     2221 ketqpsvwgq reaskpnfas vaasgvcgvp sssggsvgsv sasseplvda akapgyrgnl
     2281 hvsppnfgpi gsgprsapct ppigggapps ppsqpqpaaq pqaqefyena sapttavpqp
     2341 vqatpqpsss rlvfgpphya atapelappp qqqqqqqppq qqqtiqstln pnapdfssrs
     2401 aapvmltapf pqvgtshhhq hhhpaatpsh hgmrvpfapq eymhtvqsss hmshhhphhh
     2461 qgaagalghp gvfplqmmsh lqqqysglva pvgqmpkgad raplgvrltd egspsavspq
     2521 gglspgggld drklprpigt eraqkkgaht sypgiwsysn saagvgssgg gssltpdlsa
     2581 mqawvqyqha tgpqgdglap llqhnrfaaa aeaapeqqtl eqhtyhhqpq qpalnagglg
     2641 scppflngfp ggatgamlgg aaqlfsnrva aeaphqeatp wsphklmgsa pgdslhhhag
     2701 hhphhpqqqp phhahvatak lpwqswthhq t
//
LOCUS       XP_077506500            2774 aa            linear   INV 25-NOV-2025
DEFINITION  multiple ankyrin repeats single KH domain isoform X22 [Amblyomma
            americanum].
ACCESSION   XP_077506500
VERSION     XP_077506500.1
DBLINK      BioProject: PRJNA1367164
DBSOURCE    REFSEQ: accession XM_077650374.1
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135497) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Amblyomma americanum"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     Protein         1..2774
                     /product="multiple ankyrin repeats single KH domain
                     isoform X22"
                     /calculated_mol_wt=287392
     Region          201..466
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          201..227
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          229..260
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          265..293
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(268..269,272..274,276..277,281,284,293,295,297,
                     301..302,305..307,309..310,314,317,326,328,330,334..335,
                     338..340,342..343,347,350)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          295..326
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          328..356
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          362..392
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          376..680
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(397,401..402,405..407,409..410,414,417,426,428,430,
                     434..435,438..440,442..443,447,450,459,461,463,467..468,
                     471..473,475..476,480,483)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          397..426
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          428..459
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          461..491
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          494..526
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          528..556
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(531..532,535..537,539..540,544,547,556,558,560,
                     564..565,568..570,572..573,577,580,589,591,593,597..598,
                     601..603,605..606,610,613)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          558..589
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          591..620
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          625..655
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1107..1132
                     /region_name="ANK"
       
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